LOCUS       DAJTQY010000001        84323 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.001, whole genome shotgun sequence.
ACCESSION   DAJTQY010000001 DAJTQY010000000
VERSION     DAJTQY010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 84323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 84323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..84323
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.001"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            231..1664
                     /gene="cscA"
                     /locus_tag="OO349_000001"
     CDS             231..1664
                     /gene="cscA"
                     /locus_tag="OO349_000001"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HCR5865241.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWAFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRRISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLILWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(1672..2730)
                     /locus_tag="OO349_000002"
     CDS             complement(1672..2730)
                     /locus_tag="OO349_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR5865242.1"
                     /translation="MDLASDVCFCYSARFTANGLGMASLKDVARLAGVSMMTVSRVMH
                     NAESVRPATRDRVLQAIQTLNYVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDIL
                     LAIEQTASEFGWNSFLINIFSEDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYG
                     ENIVLANCVADDPALPSYIPDDYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQ
                     GFEQAWRDAGRDLAEVKQFHMATGDDHYTDLASLLNAHFKSGKPDFDVLICGNDRAAF
                     VAYQVLLAKGVRIPQDVAVMGFDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGR
                     EGGRVTRIPCPLLIRCST"
     gene            2898..3116
                     /locus_tag="OO349_000003"
                     /pseudo
     CDS             2898..3116
                     /locus_tag="OO349_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            3134..4462
                     /gene="dsdA"
                     /locus_tag="OO349_000004"
     CDS             3134..4462
                     /gene="dsdA"
                     /locus_tag="OO349_000004"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HCR5865243.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPSTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFAETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASCFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="OO349_000005"
     CDS             complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="OO349_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HCR5865244.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPRLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQKTMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRVLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="OO349_000006"
     CDS             complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="OO349_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HCR5865245.1"
                     /translation="MEQINSNKKQSNRKKYFSLLVIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLVLKRTDIRSPVTGY
                     IAQRSVQVGETVSSGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPDLASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            7687..8301
                     /gene="evgA"
                     /locus_tag="OO349_000007"
     CDS             7687..8301
                     /gene="evgA"
                     /locus_tag="OO349_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HCR5865246.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            8306..11899
                     /gene="evgS"
                     /locus_tag="OO349_000008"
     CDS             8306..11899
                     /gene="evgS"
                     /locus_tag="OO349_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HCR5865247.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNNDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGHNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPNLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLSPGGWDIIPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLG
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPASDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(11955..13100)
                     /gene="yfdE"
                     /locus_tag="OO349_000009"
     CDS             complement(11955..13100)
                     /gene="yfdE"
                     /locus_tag="OO349_000009"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HCR5865248.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLYNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     LGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLAKIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(13174..14118)
                     /gene="yfdV"
                     /locus_tag="OO349_000010"
     CDS             complement(13174..14118)
                     /gene="yfdV"
                     /locus_tag="OO349_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HCR5865249.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(14188..15882)
                     /gene="oxc"
                     /locus_tag="OO349_000011"
     CDS             complement(14188..15882)
                     /gene="oxc"
                     /locus_tag="OO349_000011"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HCR5865250.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPIIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(15936..17186)
                     /gene="frc"
                     /locus_tag="OO349_000012"
     CDS             complement(15936..17186)
                     /gene="frc"
                     /locus_tag="OO349_000012"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HCR5865251.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(17699..18322)
                     /locus_tag="OO349_000013"
     CDS             complement(17699..18322)
                     /locus_tag="OO349_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HCR5865252.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQTQKTAAAER
                     ISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLN
                     DDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQY
                     LMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            18619..18894
                     /locus_tag="OO349_000014"
     CDS             18619..18894
                     /locus_tag="OO349_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5865253.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDVPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(18971..19213)
                     /locus_tag="OO349_000015"
     CDS             complement(18971..19213)
                     /locus_tag="OO349_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HCR5865254.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            19566..20486
                     /gene="lpxP"
                     /locus_tag="OO349_000016"
     CDS             19566..20486
                     /gene="lpxP"
                     /locus_tag="OO349_000016"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HCR5865255.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            20841..20912
                     /gene="ypdK"
                     /locus_tag="OO349_000017"
     CDS             20841..20912
                     /gene="ypdK"
                     /locus_tag="OO349_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HCR5865256.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(20977..22215)
                     /gene="alaC"
                     /locus_tag="OO349_000018"
     CDS             complement(20977..22215)
                     /gene="alaC"
                     /locus_tag="OO349_000018"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HCR5865257.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKCYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENHDRIRQAIRGIKA
                     MFRADGLLPVSSKHIHENAE"
     gene            22592..24078
                     /locus_tag="OO349_000019"
                     /pseudo
     CDS             22592..24078
                     /locus_tag="OO349_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            24093..24827
                     /gene="ypdB"
                     /locus_tag="OO349_000020"
     CDS             24093..24827
                     /gene="ypdB"
                     /locus_tag="OO349_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HCR5865258.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            24840..25697
                     /locus_tag="OO349_000021"
     CDS             24840..25697
                     /locus_tag="OO349_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5865259.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQTQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFSLTP
                     GEYSARFQG"
     gene            complement(25700..28195)
                     /gene="ptsP"
                     /locus_tag="OO349_000022"
     CDS             complement(25700..28195)
                     /gene="ptsP"
                     /locus_tag="OO349_000022"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR5865260.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYILVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQDFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(28234..29250)
                     /gene="ypdE"
                     /locus_tag="OO349_000023"
     CDS             complement(28234..29250)
                     /gene="ypdE"
                     /locus_tag="OO349_000023"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCR5865261.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEAARLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARTYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLSCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTLVIGPATRHGHCAASIAATFCRWSNFYLPLFNVLRVR
                     RLFN"
     gene            complement(29250..30333)
                     /gene="ypdF"
                     /locus_tag="OO349_000024"
                     /pseudo
     CDS             complement(29250..30333)
                     /gene="ypdF"
                     /locus_tag="OO349_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(30348..31595)
                     /locus_tag="OO349_000025"
     CDS             complement(30348..31595)
                     /locus_tag="OO349_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HCR5865262.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAII
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(31617..31943)
                     /locus_tag="OO349_000026"
     CDS             complement(31617..31943)
                     /locus_tag="OO349_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HCR5865263.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQVLEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(32162..33127)
                     /gene="glk"
                     /locus_tag="OO349_000027"
     CDS             complement(32162..33127)
                     /gene="glk"
                     /locus_tag="OO349_000027"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HCR5865264.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDYPGLLGSGAHLRQTLGHIL"
     gene            33331..34587
                     /locus_tag="OO349_000028"
     CDS             33331..34587
                     /locus_tag="OO349_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HCR5865265.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            34791..35117
                     /locus_tag="OO349_000029"
     CDS             34791..35117
                     /locus_tag="OO349_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCR5865266.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(35258..36496)
                     /locus_tag="OO349_000030"
     CDS             complement(35258..36496)
                     /locus_tag="OO349_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HCR5865267.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLVMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            36832..38034
                     /gene="nupC"
                     /locus_tag="OO349_000031"
     CDS             36832..38034
                     /gene="nupC"
                     /locus_tag="OO349_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HCR5865268.1"
                     /translation="MDRVLHFVLALAVVVILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(38084..40196)
                     /locus_tag="OO349_000032"
                     /pseudo
     CDS             complement(38084..40196)
                     /locus_tag="OO349_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
     gene            complement(40404..40479)
                     /locus_tag="OO349_000033"
     tRNA            complement(40404..40479)
                     /locus_tag="OO349_000033"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40444..40446),aa:Ala,seq:ggc)
     gene            40700..41059
                     /locus_tag="OO349_000034"
     CDS             40700..41059
                     /locus_tag="OO349_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCR5865269.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            41061..41453
                     /locus_tag="OO349_000035"
     CDS             41061..41453
                     /locus_tag="OO349_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCR5865270.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWTREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(41505..42920)
                     /gene="gltX"
                     /locus_tag="OO349_000036"
     CDS             complement(41505..42920)
                     /gene="gltX"
                     /locus_tag="OO349_000036"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HCR5865271.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            43179..43254
                     /locus_tag="OO349_000037"
     tRNA            43179..43254
                     /locus_tag="OO349_000037"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43212..43214,aa:Val,seq:tac)
     gene            43299..43374
                     /locus_tag="OO349_000038"
     tRNA            43299..43374
                     /locus_tag="OO349_000038"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43332..43334,aa:Val,seq:tac)
     gene            43379..43454
                     /locus_tag="OO349_000039"
     tRNA            43379..43454
                     /locus_tag="OO349_000039"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43412..43414,aa:Lys,seq:ttt)
     gene            43485..43610
                     /locus_tag="OO349_000040"
     ncRNA           43485..43610
                     /ncRNA_class="other"
                     /locus_tag="OO349_000040"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            43886..44227
                     /locus_tag="OO349_000041"
     CDS             43886..44227
                     /locus_tag="OO349_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HCR5865272.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQKQQAEKAQEKEQRLSLNVSLLNP
                     VEKTTHIDIYI"
     gene            complement(44218..45144)
                     /locus_tag="OO349_000042"
     CDS             complement(44218..45144)
                     /locus_tag="OO349_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5865273.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            45234..46232
                     /locus_tag="OO349_000043"
     CDS             45234..46232
                     /locus_tag="OO349_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HCR5865274.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGARGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLSFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(46229..46447)
                     /locus_tag="OO349_000044"
     CDS             complement(46229..46447)
                     /locus_tag="OO349_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HCR5865275.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(46449..48464)
                     /gene="ligA"
                     /locus_tag="OO349_000045"
     CDS             complement(46449..48464)
                     /gene="ligA"
                     /locus_tag="OO349_000045"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HCR5865276.1"
                     /translation="MESIEQQLTELRTTLCHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGVPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKLAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(48535..49521)
                     /gene="zipA"
                     /locus_tag="OO349_000046"
     CDS             complement(48535..49521)
                     /gene="zipA"
                     /locus_tag="OO349_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HCR5865277.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYLPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            49751..50512
                     /gene="cysZ"
                     /locus_tag="OO349_000047"
     CDS             49751..50512
                     /gene="cysZ"
                     /locus_tag="OO349_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HCR5865278.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTFMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFVWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            50697..51668
                     /gene="cysK"
                     /locus_tag="OO349_000048"
     CDS             50697..51668
                     /gene="cysK"
                     /locus_tag="OO349_000048"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HCR5865279.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQVLAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            52052..52309
                     /gene="ptsH"
                     /locus_tag="OO349_000049"
     CDS             52052..52309
                     /gene="ptsH"
                     /locus_tag="OO349_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HCR5865280.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            52354..54081
                     /gene="ptsI"
                     /locus_tag="OO349_000050"
     CDS             52354..54081
                     /gene="ptsI"
                     /locus_tag="OO349_000050"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HCR5865281.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            54122..54631
                     /gene="crr"
                     /locus_tag="OO349_000051"
     CDS             54122..54631
                     /gene="crr"
                     /locus_tag="OO349_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR5865282.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(54673..55524)
                     /gene="pdxK"
                     /locus_tag="OO349_000052"
                     /gene_synonym="thiJ"
     CDS             complement(54673..55524)
                     /gene="pdxK"
                     /locus_tag="OO349_000052"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HCR5865283.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            55629..56003
                     /locus_tag="OO349_000053"
     CDS             55629..56003
                     /locus_tag="OO349_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HCR5865284.1"
                     /translation="MKKIICLAITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAEGN"
     gene            56036..56759
                     /locus_tag="OO349_000054"
                     /pseudo
     CDS             56036..56759
                     /locus_tag="OO349_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416915.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
     gene            complement(56963..57874)
                     /gene="cysM"
                     /locus_tag="OO349_000055"
     CDS             complement(56963..57874)
                     /gene="cysM"
                     /locus_tag="OO349_000055"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HCR5865285.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSCFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNAALVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(58008..59105)
                     /gene="cysA"
                     /locus_tag="OO349_000056"
     CDS             complement(58008..59105)
                     /gene="cysA"
                     /locus_tag="OO349_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HCR5865286.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNITFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQHGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(59095..59970)
                     /gene="cysW"
                     /locus_tag="OO349_000057"
     CDS             complement(59095..59970)
                     /gene="cysW"
                     /locus_tag="OO349_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HCR5865287.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(59970..60803)
                     /gene="cysT"
                     /locus_tag="OO349_000058"
     CDS             complement(59970..60803)
                     /gene="cysT"
                     /locus_tag="OO349_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HCR5865288.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLSEMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(60803..61819)
                     /gene="cysP"
                     /locus_tag="OO349_000059"
     CDS             complement(60803..61819)
                     /gene="cysP"
                     /locus_tag="OO349_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HCR5865289.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRK"
     gene            complement(62123..62914)
                     /gene="ucpA"
                     /locus_tag="OO349_000060"
     CDS             complement(62123..62914)
                     /gene="ucpA"
                     /locus_tag="OO349_000060"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HCR5865290.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     CRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(63101..63901)
                     /gene="murR"
                     /locus_tag="OO349_000061"
     CDS             complement(63101..63901)
                     /gene="murR"
                     /locus_tag="OO349_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HCR5865291.1"
                     /translation="MLYLTKIRNAESEFTGNEQKIADFLRARVSELKSVSSRQMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQHQ"
     gene            64065..64961
                     /gene="murQ"
                     /locus_tag="OO349_000062"
     CDS             64065..64961
                     /gene="murQ"
                     /locus_tag="OO349_000062"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HCR5865292.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPPAVDRV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            64965..66389
                     /gene="murP"
                     /locus_tag="OO349_000063"
     CDS             64965..66389
                     /gene="murP"
                     /locus_tag="OO349_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HCR5865293.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGDAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            complement(66569..67468)
                     /gene="yfeX"
                     /locus_tag="OO349_000064"
     CDS             complement(66569..67468)
                     /gene="yfeX"
                     /locus_tag="OO349_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708282.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HCR5865294.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDSGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(67564..68139)
                     /locus_tag="OO349_000065"
     CDS             complement(67564..68139)
                     /locus_tag="OO349_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HCR5865295.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(68200..68649)
                     /locus_tag="OO349_000066"
     CDS             complement(68200..68649)
                     /locus_tag="OO349_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HCR5865296.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(68636..69061)
                     /locus_tag="OO349_000067"
     CDS             complement(68636..69061)
                     /locus_tag="OO349_000067"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HCR5865297.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            69275..70144
                     /gene="amiA"
                     /locus_tag="OO349_000068"
     CDS             69275..70144
                     /gene="amiA"
                     /locus_tag="OO349_000068"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HCR5865298.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            70148..71047
                     /gene="hemF"
                     /locus_tag="OO349_000069"
     CDS             70148..71047
                     /gene="hemF"
                     /locus_tag="OO349_000069"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HCR5865299.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCQPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(71053..72105)
                     /gene="eutR"
                     /locus_tag="OO349_000070"
                     /pseudo
     CDS             complement(71053..72105)
                     /gene="eutR"
                     /locus_tag="OO349_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
     gene            complement(72151..72651)
                     /gene="eutK"
                     /locus_tag="OO349_000071"
     CDS             complement(72151..72651)
                     /gene="eutK"
                     /locus_tag="OO349_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HCR5865300.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVCQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(72664..73323)
                     /gene="eutL"
                     /locus_tag="OO349_000072"
     CDS             complement(72664..73323)
                     /gene="eutL"
                     /locus_tag="OO349_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HCR5865301.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            74505..75296
                     /locus_tag="OO349_000073"
     CDS             74505..75296
                     /locus_tag="OO349_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5865302.1"
                     /translation="MRALKIDAISLNFSHSPEWWRSGLIATLPRLDIERIIDTYIKRK
                     RDKFIVKIRGLHIMRISEQQRQILDYLAKNESITARQAADLVYGGNVTRVQVDAARRS
                     LMTMVSNGLIRKQGRAFVAERHINHAYSQDVEELRRQILWVFSKQGQEYFLSEHPNFA
                     AKELTVSGLSCALDYLVGEGCVTYKQLWHALNTLVEDGLLIATRENIEYWIMPRSGRR
                     NHARYISGNRYRTPDFNPTQEDRDRAEMMCREQEKRIMDKLFSMQ"
     gene            76016..76411
                     /locus_tag="OO349_000074"
     CDS             76016..76411
                     /locus_tag="OO349_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR5865303.1"
                     /translation="MTNIERLERCADIMRRRWIYDPEAGLLFSRETKDVIKGSMTNKG
                     HLVVTVHEGKFKVTLTYQKACYVYTYGAYDETLYEVHHVNLNKQDNRIKNIRLMPKEK
                     HRRIHKNVRKLIRLGHTQWLQPQLIEQSL"
     gene            76429..76692
                     /locus_tag="OO349_000075"
     CDS             76429..76692
                     /locus_tag="OO349_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5865304.1"
                     /translation="MHSLDSYFQRTTAPKSAAQERREEFHEKVMRSADYIADKFVETV
                     RPLVDEVADKLQSEMPEDMEGTAKRRLICELSRRFGVSISAFK"
     gene            76715..77257
                     /locus_tag="OO349_000076"
     CDS             76715..77257
                     /locus_tag="OO349_000076"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5865305.1"
                     /translation="MDAFEVLANKGAELVAQRDKTANEGERALLNKQIKAIRMAQFKL
                     ISNEVIETIKPQIPQIVADAIKAAGLEKRIAGLKTNAEKMEMHKQSGAPNPDEYFSPD
                     VEFMAQVEERLGSCLTEEQRRYFDGVDSSAGIDLNSYFGREIEHFDAAPSMPEPEPEM
                     ATDPEAEQRKRVFNAIGIRY"
     gene            77396..77683
                     /locus_tag="OO349_000077"
     CDS             77396..77683
                     /locus_tag="OO349_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5865306.1"
                     /translation="MLALRIMQGIAKTLAEHVLDLKHSPLSKQAMKRQTLRLWAEYSL
                     GTINKIIDMKSGPSNQSAEEMEFIRRLILIRRDIHSQLHSVGIDINDGTGD"
     gene            77722..77928
                     /locus_tag="OO349_000078"
     CDS             77722..77928
                     /locus_tag="OO349_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5865307.1"
                     /translation="MKQDIADRLEILEGQRAEAKQLRKQARRAHRNNEAELLTKYISF
                     TNYCIYECYKEDAEDWLDSLPEQY"
     gene            78240..78725
                     /locus_tag="OO349_000079"
     CDS             78240..78725
                     /locus_tag="OO349_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR5865308.1"
                     /translation="MKNKSGNSCYREAVDIHSSLELSFILHKYHLRVDEKKSRNVFSA
                     IRDELILDGDSRQNLATFCQTWLDDEIRELLNLAIDKNMIYKDEYPQMAEPEGRCVRM
                     LSDSWSFPDSRHTLGCSTIGSSEAAMLGRLALKWQWCKKREVQGKSTEPDLRSCANML
                     A"
     gene            78706..78984
                     /locus_tag="OO349_000080"
     CDS             78706..78984
                     /locus_tag="OO349_000080"
                     /inference="COORDINATES: protein motif:HMM:NF012503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5865309.1"
                     /translation="MQICWHKFCRYCDVKLCEIPLDDHHLIMNAKEVLKHVDENTIGV
                     VTTLGITYFCKADCVSVFKISVKVNISFYIYIVIICFYFKNVREYKNV"
     gene            78977..80437
                     /locus_tag="OO349_000081"
     CDS             78977..80437
                     /locus_tag="OO349_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5865310.1"
                     /translation="MSNANIAPKSKKMTVGTLAIMNITAVVSLRFIPSEAEYGLGAIF
                     YYTFAAIMFLVPVAFVAAELATTYPQKGGAFRWIGEAFGARWGFVGMLMTWLQVIPYF
                     PTVLTFGAVSVAFIDVDMGVAESIASNKWYILFFVLFVYWGAICVALRGVGIFSQVSK
                     WCGIIGTIIPAIILVILGFSYLWFSGKPAVIPLGWGDLFPNIANFQNIVLAASIFLAY
                     AGMEMNAVHVNDLDNPTKKYPIAITIASIGTIAIFLLSTLGVAFIIPTDKISLTQSLL
                     VAYDMLFEWAGVPWLGSVMAFMLAIGVLGGVVTWIAGPNTGVLAIAKAGYLPKFFQKT
                     NRHGMGHHLMFVQGIIVSVLSVTFVIMPSVQAAFQILSQLTVMLYLVMYMLMFASAIY
                     LRHSQPDTKRPYRAPALFFWATLGFLGSFLAFVLSFIPPEQIPTGSPTSYVLYLLVLV
                     ALFLLIPILIYAFRKSDWKDPNSDFEPFSWEKRTSN"
     gene            80471..81280
                     /gene="murI"
                     /locus_tag="OO349_000082"
     CDS             80471..81280
                     /gene="murI"
                     /locus_tag="OO349_000082"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001507729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCR5865311.1"
                     /translation="MFSAESPIGVIDSGVGGLTSVKEIINLLPGEDIIYCGDNMNAPY
                     GNRSADDIISLTKKMLTFLQSRNVKLVAVACNTISSTLESEEYSGYAKSFPFPILSII
                     EPAVEDVIRQQYKNVGIIATEFTIKTGCHKELIKKLNSTINVFGEPSKNLAMLIEEGN
                     LNAPAILNDIKKHVNHLISLHPVNEIILGCTHYPIVQNFFEAVAPDIKFINPAHDQAI
                     SIKNHLGQLNLLNSSNIGTLQINTSGSMEIYKTVLGELSITKPHTFSIRQF"
     gene            complement(81399..82093)
                     /locus_tag="OO349_000083"
     CDS             complement(join(81399..81847,81847..82093))
                     /locus_tag="OO349_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCR5865312.1"
                     /translation="MTSVNIHCPRCQSAQVYRHGQNPKGRDRLRCRDCHRVFQFTYTY
                     QARKPGMKELITEMAFNGAGVRDTARTLKIGSNTVIRTFKKLAPKRITSSPVAHADVA
                     FICELDEQWSYVGSKARQHWLGYAYNTKTGGVLAYTFGPRTDQTCRELLALLTPFNIG
                     MLTSDDWGSYGREVPKNKHLTGKIFTQRIERNNLTLRTRIKRLGRKTICFSRSVEIHE
                     KVIGAFIEKHMFY"
     gene            complement(82612..83985)
                     /gene="eutA"
                     /locus_tag="OO349_000084"
     CDS             complement(82612..83985)
                     /gene="eutA"
                     /locus_tag="OO349_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HCR5865313.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANYALFDAGKISG
                     TACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQLVQVTRRMAAL
                     IVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPANPFCFADIGPLL
                     ATALHDHPRLREMNVQFPAQTVRSTVIGAGAHTLSLSGSTIWLEGVQLPLRNLPVAIP
                     IDETDLVSAWQQALLQLDLDPKTDVYVLALPASLPVRYAAVLTVINALVDFVARFPNP
                     HPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPLFGGSVVPVTV
                     KSLAFPS"
     gene            complement(83960..>84323)
                     /gene="eutP"
                     /locus_tag="OO349_000085"
     CDS             complement(83960..>84323)
                     /gene="eutP"
                     /locus_tag="OO349_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HCR5865314.1"
                     /translation="GDIDTPGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPA
                     GLLDIGVSKRQIAVISKTDMPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDY
                     LASLTKQEEAGEKTHHSE"
BASE COUNT        21912 a        21339 c        20011 g        21061 t
ORIGIN      
        1 tactttggct gacatttttt tcccttaaat tcatctgagt tacgcatagt gataaacctc
       61 tttctcgcaa aatcgtcatg gatttactaa aacatgcata ttcgatcaca aaacgccata
      121 gttaacgtta acatttgcga tattcatcgc atttatgaaa gtaagggact atatttttat
      181 aaaagttaac gttaataatt caccaaattt gcttaaccag gatgattaaa atgacgcaat
      241 ctcgattgca tgcggcgcaa aacgcactag caaaacttca tgagcaccgg ggtaacactt
      301 tctatcccca ttttcacctc gcgcctcctg ccggatggat gaacgatcca aacggcctga
      361 tctggtttaa cgatcgttat cacgcgtttt atcaacatca cccgatgagc gaacactggg
      421 gaccaatgca ctggggacat gccaccagtg acgatatgat ccactggcag catgagccta
      481 ttgcgctagc gccaggagac gataatgaca aagacgggtg tttttcaggt agtgctgtcg
      541 atgacaatgg tgtcctctca cttatctaca ccggacacgt ctggctcgat ggtgcaggta
      601 atgacgatgc aattcgcgaa gtacaatgtc tggctaccag tcgggatggt attcatttcg
      661 agaaacaggg tgtgatcctc actccaccag aaggaatcat gcacttccgc gatcctaaag
      721 tgtggcgtga agccgacaca tggtggatgg tagtcggggc gaaagatcca ggcaacacgg
      781 ggcagatcct gctttatcgc ggcagttcat tgcgtgaatg ggccttcgat cgcgtactgg
      841 cccacgctga tgcgggtgaa agctatatgt gggaatgtcc ggactttttc agccttggcg
      901 atcagcatta tctgatgttt tccccgcagg gaatgaatgc cgagggatac agttaccgaa
      961 atcgctttca aagtggcgta atacccggaa tgtggtcgcc aggacgactt tttgcacaat
     1021 ccgggcattt tactgaactt gataacgggc atgactttta tgcaccacaa agctttttag
     1081 cgaaggatgg tcggcgtatt gttatcggct ggatggatat gtgggaatcg ccaatgccct
     1141 caaaacgtga aggatgggca ggctgcatga cgctggcgcg cgagctatca gagagcaatg
     1201 gcaaacttct acaacgcccg gttcacgaag ctgagtcgtt acgccagcag catcaatctg
     1261 tctctccccg cagaatcagc aataaatatg ttttgcagga aaacgcgcaa gcagttgaga
     1321 ttcagttgca gtgggcgctg aagaacagtg atgccgaaca ttacggatta cagctcggca
     1381 ctggaatgcg gctgtatatt gataaccaat ctgagcgact tattttgtgg cggtattacc
     1441 cacacgagaa tttagacggc taccgtagta ttcccctccc gcagcgtgac acgctcgccc
     1501 taaggatatt tatcgataca tcatccgtgg aagtatttat taacgacggg gaagcggtga
     1561 tgagtagtcg aatctatccg cagccagaag aacgggaact gtcgctttat gcctcccacg
     1621 gagtggctgt gctgcaacat ggagcactct ggctactggg ttaacataat atcaggtgga
     1681 acaacggatc aacagcgggc aagggatccg cgtcactctt cccccttcac gaccttcaat
     1741 aatatgcaat gcagcttccc gcccgataat gtcatgtgga agctgaattg tggtcagcgg
     1801 cggtaaaaac agatgcccga cgccaaccag attatcaaag cccattacag cgacatcctg
     1861 cgggatacgt acccccttcg ccaaaagaac ctgataagcc acaaaggctg cgcgatcgtt
     1921 accacatatc agaacatcaa aatctggttt gcccgatttg aagtgagcat tgagtaaact
     1981 tgcgagatcg gtgtagtgat catcacctgt tgccatgtga aattgtttca cctcagccag
     2041 atctcgtcca gcatcacgcc aagcctgctc aaatccctgc cgacgatacc ctgttgccaa
     2101 cgcactttcc ggtagccaga agcataatgg ttgacgatag cccgccgcga gcaaatgctg
     2161 tgttgattca tattgtgcag tgtaatcatc agggatataa ctgggtaacg ctgggtcatc
     2221 cgccacacag ttcgccaata caatattttc accatacaga gactcaggca gcgtgatatg
     2281 tcgcagcccc attgtagtat agataatgcc atccggacgg tgggcaagca gctgacgtgc
     2341 cgcgcgggca gcgtcatctt cagaaaaaat attgattaaa aaactattcc agccgaactc
     2401 gctggcggtt tgctcaatgg caagcagaat atcaacagag aaaggagtgg tagccgtgtc
     2461 ctgcgccagc acggcgagag tcgacggctt acgtccttga gcgcgcatct tacgggcgga
     2521 aagatcagga acataattca gggtctggat tgcctgcaat acgcggtcac gcgttgcagg
     2581 acgcacagat tctgcattat gcatcacccg ggagactgtc atcatcgaca ctcccgccag
     2641 gcgtgcgaca tcctttaatg aagccatacc caagccgttt gccgtaaaac gggcactgta
     2701 gcagaaacag acgtcactgg cgagatccaa cgccctatca cctgacacag caatacaata
     2761 aaaaataaca ataattcccg gacaattgtc cccaattccg cctctgttct cgccaacgag
     2821 taaccatata ggcaagtaag taaaacatgc caactggcat cgccaacctg gagaagaaaa
     2881 tataaatatg gatcattgtg ctgccactgt atcccgacat cagcccggaa attattgcga
     2941 ttgctatcgg ttcaggtgca attggctgca cgatcgttac ggactcgctt ttctggctgg
     3001 tgaagcaata ttgcggcgct acgctcaatg aaacatttaa atactatacg acagcgacat
     3061 ttatcgcttc agtcgtcgct ctggcgggca cattcctgct gtcatttatc atctaagcgc
     3121 aaagagacgt actatggaaa acgctaaaat gaactcgctc atcgcccagt atccgttggt
     3181 aaaggatctg gttgctctta aagaaaccac ctggtttaat cctagcacga cctcattggc
     3241 tgaaggttta ccttatgttg gcctgaccga acaggatgtt caggacgccc atgcgcgctt
     3301 atcccgtttt gcaccctatc tggcaaaagc atttgctgaa acagctgcca ctggggggat
     3361 tattgaatca gaactggttg ccattccggc tatgcaaaaa cggctggaaa aggaatatca
     3421 acaaccgatc agcgggcaac tcctactgaa aaaagatagc catttgccca tttccggctc
     3481 tataaaagca cgcggcggga tttatgaagt cctggcacat gcagaaaaac tggctctgga
     3541 agcggggttg ctgacgcttg atgatgacta cagcaaactg ctttctccgg agtttaaaca
     3601 gttctttagc caatacagca ttgctgtggg ctcaaccgga aatctggggt tatcaatcgg
     3661 cattatgagc gcccgcattg gctttaaggt gacagttcat atgtctgctg atgcccgggc
     3721 atggaaaaaa gcgaaactgc gcagccatgg cgttacggtc gtggaatatg agcaagatta
     3781 tggtgttgcc gtcgaggaag gacgtaaagc agcgcagtct gacccgaact gtttctttat
     3841 tgatgacgaa aattcccgca cgttgttcct tgggtattcc gttgctggcc agcgtcttaa
     3901 agcgcaattt gcccagcaag gccgtatcgt cgatgctgat aaccctctgt ttgtctatct
     3961 gccgtgtggt gttggcggtg gtcctggtgg cgtcgcattc gggcttaaac tggcgtttgg
     4021 cgatcatgtt cactgctttt ttgccgaacc aacgcactcc ccttgtatgt tgttaggcgt
     4081 ccatacagga ttacacgatc agatttctgt tcaggatatt ggtatcgaca accttaccgc
     4141 agcggatggc cttgcagttg gtcgcgcatc atgctttgtc gggcgggcaa tggagcgtct
     4201 gctggatggc ttctataccc ttagcgatca aaccatgtat gacatgcttg gctggctggc
     4261 gcaggaagaa ggtattcgtc ttgaaccttc agcactggcg ggtatggccg gacctcagcg
     4321 cgtgtgtgca tcagtaagtt accaacagat gcacggtttc agcgcagaac aattgcgtaa
     4381 tgccactcat ctggtgtggg cgacgggagg tggaatggtg ccggaagaag agatgaatca
     4441 atatctggca aaaggccgtt aataacgttt caacgcaaca tcgcaatcct ttccctgggt
     4501 gagcgatgct gccgatggcg cagacttaag atccccggtc ttacccgcta taaccccctc
     4561 cttttatcat cacccaacgc ctttcgctgt aaacggcggt ttcgcaaacc aaaccaaaac
     4621 cgtaagtaag ataaaagcaa tagccgccat acggaaaatc tcatttgcag aaatagaaag
     4681 tgactgctgg gttatttcat tattaatttc attaagaact cctgaaagcg aaccatagta
     4741 tttatccata atttgtgacg atgaattaaa cacggggtta aattgatcga tggttgctgt
     4801 caactgacta tggtgtaacg attcgcgtcg tccccacagc gtcattgtca acgacgtacc
     4861 aactgatcct gacaaggtac gaaaaaaatt actcatactc gaggcattgg caaatttatt
     4921 atctggcaag cctgaaaacg aaatcgttgt taagggtaaa aagaaacagg caacggcgaa
     4981 tccctggaaa aactgtggca aaatgatgcc tgtaaaatca atcgttggca taaatgtcac
     5041 agaacgccag taatagcaaa ccgcatacat caaaaaactg aatgtcacta acacccgcat
     5101 gtctatttta ttgccataac gtcctatcaa aggtgaaatc aatagtggca tgatgccgat
     5161 gggcgcataa gcaagcccgg cccatatcgc attatacccc atcgttttct ggagtaactg
     5221 cggcataagg acgatcgctc cagagtaaaa taaatacgcg caggtgatac tcacaatacc
     5281 aatggtgaaa ttacgggact taaacaaact gagatcaaga atcgggttct ctgaggtcga
     5341 ttcccaaatg actaaagaga tcagagaaat aactgatact actgttaata ttattattgt
     5401 actcgagttg aaccaatcca gatcgcgccc tttatcaagc ataatttgca agccaccaac
     5461 accgagcact aacagggtca gtcttggtag attcattttt accggtgaag tttcagtttc
     5521 tcttccttta agtaaggtta agcataatgt caggacgata atccccatag ggacattgat
     5581 taaaaatatc caaccccagc taaagttatc gcaaatatag ccgcccaata tcggcccaca
     5641 tatcggagcg ataatcacgg tcattgacca taatgccaga gcaaatgttc ttttttctgg
     5701 tggataattc cttaataata aactctgtga cagtggaatt aacggccccg ccattaatcc
     5761 ctgaacgact ctaaaaaata tcagcacatc aagattggtc gataaactac acattaagga
     5821 agacagcgaa aaaaaagtga ctgaaagtaa aaataacctt aattcgccta ttctttgtgc
     5881 cagcctgccg gtaacaggga tcgcaatggc atttgctacg ccaaacgagg tgataaccca
     5941 ggtgccttcg tctgttgatg ctcccagaaa gccagatatt gtcggtattg cgacgttaga
     6001 aatagtggaa tccaacattt gcataaacgt cgctaatgac aatgcaatag taacgcacca
     6061 taacgtccca ccggttaatg gtgccggagt tgatttagtg attgccatta aagttgtcca
     6121 ttatgcgaaa taatgttgct aatttctttt tctatcggac tggtatcgat aactaaagcc
     6181 ttactggtat aagcaggcat ggaggtcacg gttgaagcca gatcaggcat ctcagcaatg
     6241 tcttcgtcct tcgtatcaat agttgcagtc atcgataaac caatgcgcaa ggggtgttcc
     6301 atgagttctt ttggatcaag agaaacttca accggtacac gctgaacgat tttgatccag
     6361 ttccctgtcg cattttgtgc aggtaataag gagaacgcat tgccggttcc catattgatc
     6421 cctgtcaccc gaccatgaaa cacaacattt tcaccataaa gatcgctgat aatattgact
     6481 gattgaccaa tccgtacatc cgtgagttgt gtttctttaa agttggcatt aacccacatt
     6541 tgacgtgccg gtacgacagc cattaacgat tgtccggaac tcactgtttc gccgacctga
     6601 acacttcgct gggcaatata gccagtaacc ggactcctta tatccgtacg tttaagcacc
     6661 aaccaggctt ctttggttgc atccgccgct tcaactactt gtggctgacg gtttaatggt
     6721 gtgttcatta ctaaagcttt attcgcttta taagcctgga tagcggcatt cagtgccgct
     6781 ttgctactta ttaacgtatc tttggtatgc tccagcgttt cttttgaaat aaccccctgc
     6841 ttcgctaaag gcacacgacg gttataatct tctaaagatt gttgatactg aatacgtgct
     6901 gaagcgactt cggcactgta ttgtttatcc tgtaagtaca gtttattcgt ttgccgaaca
     6961 atatttgcca gattattttt agctttattg agtgcgatag tggcatcagt tttatccagt
     7021 gaaactaaaa tgtcaccctg tcgaacgtag ttcgtatctt tatgattaac tacagtgaca
     7081 ctacctgaga cttgtgcaga aattggatct gcattccccg tgacataggc gtcatctgta
     7141 ctaatcatgt cttctaattc cattgaccaa taggcatagg cacctgaaaa cgcaataaat
     7201 aaaactatca ccaataaaga aaagtatttt tttctgttag actgtttttt atttgaatta
     7261 atctgttcca ctattatctc tcatttcgca tagatgaaat ttatgaattg aatgaaaaga
     7321 gtattcattc ttcatatcaa acacagtgca tgtgttgcac attatcccag gaacgttgga
     7381 ggcctaattt aaatgaggtg caaaaaaaac aacagaggac taaaccgtgg cttttgcaat
     7441 acaattctta cgcctgtagg attagtgaga agacttatag tgctaacttg aaactataaa
     7501 tcatcggtat aatccctgat tttattgttg acatttcatt tatgccgact atttatatgg
     7561 tatacttgtc gaattatctt aaaggaaact cagattttct tatttttatt gagaaaatga
     7621 gatgacgcct tatgtctgta ttactacagg gagaagggag atgcttcatt gcaaagggaa
     7681 taatctatga acgcaataat tattgatgac catcctcttg ctatcgcagc aattcgtaat
     7741 ttattgatca aaaacgatat tgaaatctta gcggagttaa ctgaaggcgg aagcgccgtt
     7801 cagcgggtgg aaacacttaa gcctgatata gtcatcattg atgtcgatat ccccggagtt
     7861 aacggtatcc aagtgttaga aacgctgagg aagcgccaat atagcggaat tattattatc
     7921 gtctccgcta aaaatgacca tttttacggg aaacattgtg ctgatgctgg cgctaatggt
     7981 ttcgtgagta aaaaagaagg catgaacaat atcattgcgg ctattgaagc tgcaaaaaat
     8041 ggctactgct atttcccctt ctctctcaac cggtttgttg gaagtttaac gtccgaccag
     8101 caaaaactcg actccttatc gaaacaagaa attagtgtca tgcggtatat tcttgatggc
     8161 aaggataata atgacattgc ggaaaaaatg ttcatcagca acaaaactgt cagcacgtat
     8221 aaaagtcgcc tgatggaaaa attagaatgt aaatcactga tggatcttta cacattcgcg
     8281 caacgtaaca aaatcggcta atcacatgaa gtttttaccc tatttttttc ttctctgttg
     8341 tggtctttgg tcgaccataa gtttcgcaga cgaagattac atcgaatatc gtggcatcag
     8401 tagtaacaac cgtgtcacac ttgatccagt acgtctgagc aacaaggaat tacgttggtt
     8461 agcgagcaaa aaaaatcttg tgattgcagt acacaagtcc caaacggcta ccttgttgca
     8521 taccgattcg cagcaacagg ttcgtggtat taatgctgat tatttaaatc ttttaaaaag
     8581 agcgttaaat atcaaattaa cactccggga atacgcagat catcaaaaag caatggacgc
     8641 gcttgcagaa ggtgaagtcg atatagtgtt atcacattta gttacttcgc cgcctcttaa
     8701 taatgacatt gctgcaacca aaccattgat aattaccttt ccggcgctgg taaccaccct
     8761 tcacgactca atgcgaccgc ttacctcacc aaaaccagta aatattgctc gggtagcaaa
     8821 ttacccccca gacgaggtaa ttcatcaatc atttccaaaa gcaacaatta tctcttttac
     8881 aaatttatat caggcattag catccgtctc agctgggcac aatgattact ttattggtag
     8941 taacatcatt accagcagta tgatttcccg ctatttcact cactccttaa atgtagtgaa
     9001 atattataac tcgccgcgtc aatataattt tttcttgacc agaaaagaat ctgtcattct
     9061 taatgaagta ctcaatagat ttgttgatgc tttaacaaat gaagttcgct atgaagtatc
     9121 acaaaattgg cttgatacag gaaacctggc ctttctgaac aaaccattag aactcactga
     9181 acatgaaaaa cagtggatta agcagcatcc caatttaaag gtgctggaaa atccttactc
     9241 gcccccctat tctatgacgg atgaaaatgg ctcggttcgg ggcgttatgg gggacattct
     9301 taatattatt accttgcaaa caggtttaaa tttttctccg atcaccgttt cacacaatat
     9361 ccatgctgga acacaactta gccccggagg atgggatata atacctggcg ctatttatag
     9421 tgaagatcga gaaaataatg ttttatttgc tgaagccttc ataacaacgc cttacgtttt
     9481 tgtcatgcaa aaagcgcctg acagtgaaca aacattaaaa aaaggaatga aagttgccat
     9541 tccatattat tatgagctgc attcgcaatt aaaagagatg tatccggagg ttgaatggat
     9601 acaggtcgat aatgccagcg ctgcatttca caaggttaag gaaggtgaac ttgatgctct
     9661 ggtcgcgaca cagctaaatt cgcgttacat gatcgatcat tactatccta atgaacttta
     9721 tcattttctt attcctggcg ttccgaatgc atcgctttcg ttcgcttttc ctcgcggaga
     9781 accggaactt aaggatatta ttaataaagc actgaatgca attcccccaa gcgaagttct
     9841 gcgcctgacg gaaaaatgga ttaaaatgcc caatgtgacc attgacacat gggacctata
     9901 tagcgagcaa ttttatattg ttacgacatt atccgtttta ttagttggca gtagcctttt
     9961 atggggattc tacctgttac gctcagttcg tcgtcgtaaa gtcattcagg gtgatttaga
    10021 aaaccaaata tcattccgaa aagcactctc ggattcctta ccgaatccaa cttatgttgt
    10081 aaactggcaa ggtaatgtca ttagtcataa tagtgctttt gaacattatt tcactgcgga
    10141 ttactacaaa aatgcaatgt taccattaga aaacagtgat tcacccttta aagatgtttt
    10201 ttctaatgcg catgaagtca cagcagaaac gaaagaaaat cgaacaatat acacacaggt
    10261 atttgaaatt gataatggca tcgagaaaag atgcattaat cactggcata cattatgcaa
    10321 tcttcctgca agtgacaatg cagtatatat ttgtggttgg caagatatta ctgaaacgcg
    10381 tgatctaatt aatgcactcg aggtagaaaa aaataaagcg ataaaggcta ccgtagcaaa
    10441 aagtcagttt ctggcaacga tgagtcacga aataagaaca ccaataagct ctattatggg
    10501 cttcctggaa cttctgtcgg gttctggtct tagcaaggag caacgggtgg aggcgatttc
    10561 acttgcctac gccaccggac aatcactcct cggcttaatt ggtgaaatcc ttgatgtcga
    10621 caaaattgaa tcgggtaact atcaacttca accacaatgg gtcgatatcc ctactttagt
    10681 ccagaacact tgtcactctt tcggtgcgat tgctgcaagc aaatcgatcg cattaagttg
    10741 cagcagtaca tttcctgatc attacctggt taagatcgac cctcaggcgt ttaagcaggt
    10801 cttatcaaat ttactgagta atgctctcaa atttaccacc gagggggcag taaaaattac
    10861 gacctccctg ggtcacattg atgacaacca cgctgttatc aaaatgacga ttatggattc
    10921 tggaagtgga ttatcgcagg aagaacaaca acaactgttt aagcgctaca gccaaacaag
    10981 tgcaggtcgt cagcaaacag gttctggttt aggcttaatg atctgcaaag aattaattaa
    11041 aaatatgcag ggcgatttat cattagaaag tcatccaggc ataggaacaa catttacgat
    11101 cacaatcccg gtagaaatta gccagcaagt ggcgactgtc gaggcaaaag cagaacaacc
    11161 catcacacta cctgaaaagt tgagcatatt aatcgcggat gatcatccga ccaacaggct
    11221 attactcaaa cgccagctaa atctattagg atatgatgtt gatgaagcca ctgatggtgt
    11281 gcaagcgcta cacaaagtca gtatgcaaca ttatgatctg cttattactg acgttaatat
    11341 gccgaatatg gatggttttg agttgactcg caaactccgt gagcaaaatt cttccttacc
    11401 catctggggg cttacagcca acgcacaggc taacgaacgt gaaaaagggt taagttgcgg
    11461 catgaactta tgtttgttca aaccgttgac cctggatgta ctgaaaacac atttaagtca
    11521 gttacaccag gttgcgcata ttgcacctca gtatcgccac cttgatatcg aagccctgaa
    11581 aaataatacg gcgaatgatc tacaactgat gcaggagatt ctcatgactt tccagcatga
    11641 aacgcataaa gatctacccg ctgcgtttca tgcactagaa gctggcgata acagaacttt
    11701 ccatcagtgt attcatcgca tccacggtgc ggctaacatc ctgaatttgc aaaagttgat
    11761 taatattagc catcagttag aaataacacc tgcttcagat gacagtaagc ctgaaattct
    11821 tcagctgctg aactctgtta aagaacacat tgcagagctg gaccaggaga ttgctgtttt
    11881 ctgtcagaaa aatgactaaa ttgcggctcc cacaatgttc taatgtggga gctatttacc
    11941 agcacatctt acatttatga tgagaactcc tggcgaattt gttccccatg ctggtcgagc
    12001 gttgccgctc ccggcataac atgcgggtcc gcgcagccgc tgatttttat cggattaccc
    12061 ggcatcatta ttcctccggc ttcaatcaac atatttctcg cctgagtttg tggcaaatta
    12121 atggcctcag ccacacttaa taacggcgcg acgggcacac caacttcatg tattttggct
    12181 aaccaaactt cagctgcctg cgtttttaac gttcgctcaa tatattgttt aagaatagcc
    12241 tggttttgta cgcgtaaaat attgctgcta aatcggggat cattaaccag ttccgtaagc
    12301 tccagtgcct ggcataacgc agaaaaaagc ttgtcattac cacaacaaat cgtaatcggc
    12361 ttatcctgag tatcgaaaac atcaaaaggt gccatgtagg gatggcgatt tcccaggcgt
    12421 tgtggtgact tccctgtcgc aatatatgcc atcagaccat gctccagaaa actcagcgtg
    12481 gcatcaaaca tcgctatatc gacatgcgcc cctctctggc tcttttcgcg gccataaagt
    12541 gcactcacta ttccgctgaa taaatagaca ccgccgcata gatccgcaag agaggtacca
    12601 acacgcactg gcggagcatc agggtaccct gtttccatca ttatcccact cattgcctga
    12661 atgatggtat cgtaggcagg agcatctttt agcggaccgg tatgtccgaa acctgacgat
    12721 gaagcatata tgaggcgcgg gttgatttct tgtaaccttt cccatgaaaa ccccagtttt
    12781 tccattgtac ctaggcgaaa attctcagct aatacatcag cttgtttgag catatttata
    12841 aatatacttt tatcgtgatc attctttaaa tcaagaacca cactctcttt gccatgatta
    12901 ataaaactgt aatagagtga ctgtccatcc acatagggac caaatgtgcg ggtatcatca
    12961 ccatgacctg gtggctcaac tttaattacc cttgcgccca tattataaag aagttgagtt
    13021 ccgaaaggtc cattaaggac atgtgtcata tcgataacta ataagccttc aaacggccct
    13081 ttgctttcat tatttgtcat ttgcttatgc cttgaaaaaa gccgaaggtg aaaagacact
    13141 tcggcattta tgtaaaatca ataacatgat taattatgaa accagtcgac tgacataaat
    13201 ccacaaggga gccgtgacga caaagcccag tacgctcaca gccaatgacg ctgtaccagt
    13261 acgggtgtag acattaaacc ggctggcaat aatgattccg gagaatgccg gcggtaatgc
    13321 gcctgccagt accatcatct gcaaatgttc gctgttcaaa tgacatgcca taccaacgag
    13381 aagcagcgcc agtggcatca gaatcaactt caggaaggtg ttataagcaa tttcggcact
    13441 gaactcgaat ttatgtgcag ccagagtcaa ccccgcagcg aataccgcta cccctgagtt
    13501 agccttcgca atcagattaa aggttgggtc ccatgctgcc ggaattttta ccccaaccag
    13561 caccaggatc gttgccagaa caggtgccca tactactggc tcctttgccg cagaaattaa
    13621 tgcgctcaga ttactattct tcttaccatc cgctcctgag gaaggattca gcaaatacag
    13681 accaatagga atagtaattg cgttaacaat aatagaaata attgctacca ctaaacctgt
    13741 tgatacggaa tcaccataaa taggatcgag aactgcaaac cccaggaatc caatggtagg
    13801 tgaacccgca attaatgcac atacagctgc ttctgcatgg gtacgtttaa aaaatttgta
    13861 gcaaccaaac caggagaaaa agaaacatcc gacaataacc acaagtgata caagggtcag
    13921 acgagtgtcc gcaaaaatca tttccctgtt tgcccgggta atagaaacaa atagagccgc
    13981 aggaagagcg tagtttaata ccagtttatt aaatgcgcgg gcttgatctt ctgaaaaagt
    14041 ttctcgtctg ccgctaaaat aacccaacag catgattacg ataataggca ataaatcgcc
    14101 aataaaaaat gttagcataa tagtatttcc gataagggtc ccctcaccat cgcataatga
    14161 gttggcaaga ggagacgatg tcagtattta attaccagcg acttgttttg ggttaagttt
    14221 ggtaatatgg ccactttcag ttcctgctgc agggtcgata accacattaa taatggtcgg
    14281 tttgcgcgac tgaataccgg tggttaaagc atgacgaagt tcatctgtcg tggtgacgtt
    14341 atagccaacg ccacgaaacg catccattaa tttgtcatac cttgcatggt gcaacagatc
    14401 cgttggtgat ggtgcaccag cgccactgag atcaacaccg tctcctctgt agatgccgcc
    14461 attattaaaa ataacgatcg tcaccggcag gttatatcga caaatcgttt caatttccat
    14521 cccactgaaa ccaaaagcac tatcaccttc aatggcgaca accggagaac cagaagtcac
    14581 gctagcaccg atggcatagc ccataccgat gcccatgaca ccccaggtac cacaatccag
    14641 acgacgacgt ggtttataca tatcaataat atttcgtgca ttatccaggg tatttgcacc
    14701 ttcattaact aaataaatat cctggttctc gcgcaatacg tcgcgcacag cacttaatgc
    14761 attaaagtaa tttaatggtt gagtatctgt acttaatttt tcatgcattt tttgtgcatt
    14821 ttgctgcttg tggatattta aaatatcgcg ccataccagt ggagtcgtaa atgtgttttg
    14881 tttcagttct gccagcatac cttgcatact ggatgcaata tcaccaacga ctggcacagc
    14941 aatggggcgg ttgctgtcaa tttcctgcgg ttcaatatcc agttgaataa actgtgtatc
    15001 tgccgcccat ccttttttac cgtgtgccaa taaccaattc agtcgtgcac caacaagcat
    15061 gacaacgtca gcatttgcca gggcaaacga acgcgcagct gccgcagaaa gtggatgcgt
    15121 atcttcaagg atccctttcg ccatagacat tggcaggaat ggaatctgag cactttcaat
    15181 aaattcacga agctgttcat cagcttgtga atacgccgcg cctttgccaa ggataattaa
    15241 tggccgttca gcttttgcta aaagcgaaat tgcgctagtg actgacttcg ggcatggcaa
    15301 taatgctggc gacggatttt caactttaac aatcgtggtt aacgcttcgt ctttttccat
    15361 cgtcgcggcc aggacatttg ctggcaaatc aagataaact ccgccagggc gacccgatac
    15421 agagacccgg atagcgcgtg ccaatgcaat gccaagatcc tgcggctgat taacgcgaaa
    15481 tgctgctttg gcatacggtt ttgccgcatt catttggtcc agctcttcat aatcaccttg
    15541 ctgtaggtcg acgatcgcgc ggtcgctgga gccgctaatc atgataatcg gaaaaccatt
    15601 taccgttgcg ttggccaatg cggtcaaacc attgaggaat cctggcgcag aaactgtcag
    15661 gcagatcccc ggtttttggg taagaaaacc gcttgccgca gcggcatagc ctgccgactg
    15721 ctcatgacga aaaccaatat aacgaatgcc ttccgcctgg gcatggcgtg ccatatccgt
    15781 cacaggaata cctacaacac cataaatagt gtcaatatta ttctgtttta atgcttcaac
    15841 gatgatatgc ataccatctg tcatttgaag ttgatctgac atgattaata acctcgaaat
    15901 ttttataaat tgggagccag aagcccccgt taatatcaga tggcgtggtt ttgcttcatt
    15961 gcagcaattt catcgtcgct ataacccagc tcctgcaata cagcagcggt atgttcacct
    16021 aatagcggcg cagctttaat atccggcgta aaggcagaga atttcattgg acaaccaacg
    16081 gtcagatatt ttccacgcaa cggttgttcc acttcaacaa cactgccact ttggcgcaaa
    16141 gagggatcaa gtgaaatttc tttcatactt aaaaccggtg cacaaggaat atcaaactga
    16201 gtcaaatagg ccaccgcttc atgtttatca atagtgacag tgtatttttc gatttcagca
    16261 aaaatatcga aaatatgtgg ctgtcgggca tgggctgtac tgtatgccgg atcggtaatc
    16321 cattctggtt tgccgatggc tttacaggtg ttttcccagt tttgctcctg aatagtgaaa
    16381 taaatatagg cgttaggatc ggtttcccag cctttacatt tcaggatcca gccaggctga
    16441 ccgccaccac ctgcattacc accgcgggga actgcatcac caaatgtacc attcggatac
    16501 tgcgggtatt cttccagata acccaattta tcgagacgct gctggtcacg taatttcacg
    16561 cggcaaaggt tcaatacggc atcctgcatt gacatggtga ctcgttgccc acgccccgtt
    16621 ttttcgcgat gcagcaaagc agcaagtaaa ccgatcagca aatgcattcc ggtgttgctg
    16681 tcacccaacg ctgcagcgct taccagcggc ggaccatccc aaaaacccgt agtggatgcc
    16741 gcgccacccg ctgcctgagc aacgttttca taggctttta cattcacata aggcgaacac
    16801 tcatcaaacc ctttgatcga accaaaaatc agacgtggat tgatttcttg aatatgctcc
    16861 caggtgaagc ccatgtgatc aatggcccct ggatgaaagt tctcgactaa gatatcagct
    16921 tcgcggatca gcttttccat tacctctttg ccttccgctg ttttggtatt taactcaata
    16981 gaacgtttgt tactgttaag catggtgaag taaagcgcat cgatatcagg aatatctcgc
    17041 agttggtgac gcgttacgtc accaacgccg ggacgttcaa ttttaataac gtcagcgcca
    17101 aaccaggcca gcatttgagt acaagatggg ccagattgca caccggtgaa atcgagaact
    17161 ttaattcctt gaagtggagt tgacatagca atacctatta gttaattaaa tatgtgttaa
    17221 tttagcgttt atttaaggca taacgccctc tataaataat ctgcaaaata tcttcgtagc
    17281 ggcactcgat atttttaaat tcctgtatca agtactgaaa attattttat cgccacttcc
    17341 aattcctttc tgcatggtaa gtagtgagga cgaaaagaag aatatgataa atattgattc
    17401 gtggtaatca gagagagtcg cgacatttgt gatgttattc ttacaactgt aagaagcgcc
    17461 ggtgatttac aagtaagagt atgattacgc tttttaaagc gtaccataaa gcataacata
    17521 ataaatgatt tgtgatgaca cgggattatt gcggtttact ttgaggaaaa taaaatgcgt
    17581 gtttatagaa ccgcgagcgc caaataattt tatgaccaca acctgtcgta ttgtggtcat
    17641 aaaaaattgt cggctggagg gagttaaatc ctttacctca atatactgcc tgtcagcatt
    17701 agttcacaaa cagtgcttcg ctatcgacga tgattccatc ttccgcacct ttaagcgcca
    17761 ggtttgcctc gtagtattgc tttttatcaa gcagtttctg cgcatcagca agtgcattac
    17821 gagtctgttt aagcggcatc agatattggt tttccattac gccaacaccg gccaggcgaa
    17881 gctcttccat tgcgcccttt ttatcccctt tcgccatttt ttcattggca atttttatcg
    17941 cggcttcttt ttcgggtgtt gctacataac tctcagaaat tccgacagat gcattaatga
    18001 caatatattg gtcatcattg agattggtct ttttacccgg cttagcgaac tttgcccatt
    18061 cggtactgtc atctgacagc aaagcggaag cttcattggt cagttctttt gccttttccg
    18121 gatcgccatg aaacagagcc aggcgcgcta cctgcacgtc acgcatcgca tataaacctt
    18181 gttcagagat acgctctgct gctgccgttt tctgggtttg ctgagtttgc tgttgtgccg
    18241 ctaccggtgc attatcagca gcccatacag ttgaagatgc cagaattgct gtgaccatcg
    18301 tagccataat taaacgtttc atacttacct cattctttaa cagttttaga aaacgccgcc
    18361 atcatattgg gtgagcatga aatgagaaat cagagagcaa cggtcatttt gaaagggtta
    18421 tcttacaatt gtagttatta tcgcaaagcg gcatttagtc ttacagttgt aggaatatgc
    18481 ctcctgtcgc agagaactat tcttatatac cacaaagaga tgtcatttat tatattgatg
    18541 taaatattga ttaattatat gtaaatacag ccataacatc gtatggttct cagtgaacca
    18601 ttttcaggat aactttctat gaaagtaaac ttaatacttt tcagcttatt tttattggtc
    18661 tctattatgg catgcaatgt ttttgcattt tccatttctg gtggtgtaag tgagaggagc
    18721 tataaagaga ctgaaaaaac atcagcgatg acgaccacac actctacaaa acttcagcca
    18781 tcacaggcga ttttgtttaa gatgagagaa gatgtgccac cattaaacct cacagaagaa
    18841 atcacgccta cttatccgac aaaggcgaat tatcttattc atcctgtgcg atagcgtctg
    18901 tcacatggca ggtgaacaat catgtctgat gctgatgtga acacctgccc aaatgagcca
    18961 tcctgaccca ctatcgcctg ccatacccca ccataaagcg aatcatcaag ccacaagccg
    19021 gggcaagaaa cataaatatc cccgtcacca gttcattacc gccagttaac cacatggtga
    19081 agtaataaat gagtgcagcg aggatcacca tgccgaagaa agaagagacc gccgatacca
    19141 cgttcagtac ctgaccgatg tagccgctta cgcagacaat acacagggcg agaaacatcc
    19201 ataaataaat catagctcaa cctcctgccg cgctaaaaaa cctgtaactt cagtcacttt
    19261 aatagtaaga caatccttaa cctctggcaa tttttgctaa gcatcaatga aaacagcacg
    19321 ttaaatgcaa cgctattgcg tgcggaaaat tttttacttt gcacgattaa taatcagtag
    19381 ctgaaagcag tcagccagtg ataaagtaga gccaccgcat tcatgctcct cgtggttatg
    19441 tcctgactag tctttacact ctttacagga accattgtcg tacatgatgg cccaaccaat
    19501 tgagtgtacc gctgtcgaca tggaatcttc tgcgatacaa caattcgtat ctacagaagg
    19561 taactatgtt tccacaatgc aaattttccc gcgagtttct acatcctcgc tactggctca
    19621 catggtttgg gcttggtgta ctctggcttt gggtacagct tccttatcct gttctctgct
    19681 ttctcggcac gcgtattggc gcaatggcgc gaccattcct gaaacgtcgt gaatctatcg
    19741 cccgtaaaaa cctggaactt tgtttcccgc agcattctgc ggaagaacgc gagaagatga
    19801 ttgccgaaaa ctttcgttca ctcggcatgg cgctggtaga aaccggcatg gcatggttct
    19861 ggcccgacag tcgcgtacgt aaatggtttg atgttgaagg gttggataac cttaaacgcg
    19921 cacaaatgca aaatcgcggc gtaatggttg tcggcgtcca ttttatgtcg ctggaactgg
    19981 gcggccgcgt gatgggactg tgccaaccaa tgatggctac ctatcgtcca cataataatc
    20041 agctgatgga atgggtgcag acccgtgggc gcatgcgctc taacaaagcg atgatcggca
    20101 gaaataatct gcgcggcatt gtcggtgcac tgaagaaagg tgaagcggta tggtttgctc
    20161 ccgatcagga ttatggtcgt aaaggcagct ccttcgcgcc gttctttgcg gtggaaaatg
    20221 tcgccacaac caatggcacc tatgttctct cccgtctctc tggcgcagcc atgttgaccg
    20281 taacgatggt aagaaaagcg gattacagcg gatatcgttt gttcatcacc ccagagatgg
    20341 aaggctaccc gacagatgaa aatcaagccg ctgcttatat gaacaagatt atcgagaaag
    20401 agatcatgcg cgcaccggag cagtacctct ggatccaccg tcgctttaaa acgcgcccgg
    20461 tgggagaatc gtcgttgtac atttaacaaa ctgctcttac tctttccctc ctcaaatcgg
    20521 ccatgataac ctcatgattt ttatagcttt tttcttaagt tttttccttc gctgaatatc
    20581 taattattac cgtcagaaat taaactgtcg ccgatatgag acacttgtaa gttactaaaa
    20641 gtaaataaga aaatgcctct tgtctcgttt cagttttagg cgtacattag cgccgtctcg
    20701 agcagtttag ctcagaattc tggggtgatg cccacggtaa aatacagggc ttttaaggtc
    20761 gctttgcccg gtgtcaactc actgtatcag gtgtaatgaa gtcattcagg cgtaacagta
    20821 attacgcgga gagatgtaaa gtgaaatatt tctttatggg catttctttt atggtcatcg
    20881 tttgggccgg tacttttgcc ctgatgatct aaaagcaaac tgtgcaatga aaaacaggag
    20941 ccatcaggct cctgtttgca tttctgacga cggtgtttat tccgcgtttt cgtgaatatg
    21001 tttgctgctg acgggtagta aaccgtcggc acggaacatc gctttaatcc ctctgatggc
    21061 ctgacgaata cggtcgtggt tttcaatcag tgcaaagcga acatgcgtat caccatagtc
    21121 gccaaagcca atccccggcg agacacaaac cttcgcttcg ttaaggagct tcttggcaaa
    21181 ttccagcgat cccatggccg catatggttc cgggattttc gcccagacat acatcgaagc
    21241 cttcggcatt tcgaccatcc agcccgcttc atgcagccct ttaaccaata catcacgacg
    21301 gcgtttgtac tgttcggcaa tgtcgcgcac gcactgttga tcgccctcga gtgccgcaat
    21361 cgccgccacc tgcaacggcg taaaagtgcc gtaatcgtga tagcttttga ttcgcgccag
    21421 ggcactgacc agcgttttgt tgccgaccat aaagccgata cgccagcccg ccatgttgta
    21481 gcttttcgac agcgtaaaga actcgaccgc cacatcgcgt gcacccggca cctgcataat
    21541 tgacggcgct ttccagccat cgtagacgat atcggcatag gccaggtcat ggaccaccag
    21601 cacatcgtag catttcgcca gcgccacaac cttttcgaag aactccagtt ccacgcattg
    21661 cgcggttggg ttcgacggga agccgaggat catcatcttc ggtttcggat aactttcacg
    21721 aatggcgcgc tccagttcgt tgaagaaatc gacaccttcc accagcggca ctgagcgtac
    21781 ctgcgccccg gcaatcaccg cgccataaat atggatcggg taacttggat tcggcaccag
    21841 caccgtgtca ccatgatcca gcgtcgccag catcagatgc gccaggccct ctttcgaacc
    21901 aatagtgacg atggcttctg attccgggtc gatttcaacg tcgtagcgat cctgatacca
    21961 gcgggaaatg gcgcgacgta accgcggaat gccgcgtgaa gtggagtaac catgcgtgtc
    22021 cgggcgctgg gccacagtac ataatttttc gacgatatgc ggcggagtcg caccgtccgg
    22081 gttacccatg ctgaaatcga taatatcttc gccgcgccga cgcgcagcca ttttcagttc
    22141 agcggtgatg ttaaaaacgt agggcgggag acgatcaatg cgcgtaaagc gacgttcagg
    22201 gcgagtgtca gccataattt tctcagatta acgttagcgc ccggaccgtc cgagcgacgc
    22261 tgccacaatg gtggctgttt tgaaaatagc ctgattaatt tctgtctgtc cagcgtttta
    22321 gaacattttt atcaatggat ttaaatgagg aactccactt caaaaacagg aaatttttct
    22381 acgctaacaa gaaaatcacc accaatttta ttaacattat aatttcaata aatttaccag
    22441 ctagaagtgc attatcaaga gatacattgc taatacacca tttatctttc aatccccttt
    22501 taaatttatg gtttttcctc aaaaacggcc tgctggtcat cagccttcag gttacctatc
    22561 atagaggttt aatccttatt cagagtcacc cgtgcacgaa atattcaaca tgctgctggc
    22621 ggtcttcgat cgggcagcgt taatgcttat ctgcctgttc tttctcatcc gtatccgcct
    22681 gtttcgcgaa ctgttgcaca agtcggcgca ctccccaaaa gaattgctcg ccgttaccgc
    22741 cattttctcg ctgttcgccc tgttcagcac ctggtccggc gttcccgtag aaggctcgct
    22801 ggtgaacgta cgtattatcg cggtgatgtc cggcgggatt ctgtttggcc cgtgggtagg
    22861 catcattacc ggcgtgattg cgggtattca ccggtattta attgatatcg gcggcgtgac
    22921 ggcgatcccc tgctttatca ccagcattct ggcgggttgt atatcaggct ggataaacct
    22981 gaaaatcccc aaagcacagc gctggcgctg gatatcgcca acaaaacgct accgctgttt
    23041 cgccatgtca atagcgagtc attacgcaag gtctgcgaaa ttatccgcga tgacattcac
    23101 gccgatgcgg tggcgattac taataccgat catgtgctgg cctatgttgg cgtgggtgaa
    23161 cataactatc agaatggcga tgacttcatt agcccgacta cccgtcaggc gatgaattac
    23221 ggaaaaatca tcattaaaaa caatgatgaa gcccaccgca caccagagat tcattccatg
    23281 ctggtgatcc cattgtggga gaaaggggtc gtgaccggaa cgctgaaaat ttactactgc
    23341 cacgcgcatc agatcacctc gtcattacag gaaatggcgg tcggtctgtc gcaaatcatc
    23401 tccacgcaac tggaggtttc acgcgccgag cagctacgtg aaatggcaaa taaggcagag
    23461 cttcgcgccc tgcaaagcaa aattaatccc cattttctgt ttaacgctct gaacgctatt
    23521 tcatcgtcaa tccgtctgaa tccggatacc gctcgccagt tgatctttaa tctgtcgcgt
    23581 tatctgcgct ataacattga attaaaagac gatgagcaaa tcgatatcaa aaaagagctg
    23641 tatcaaatta aagactatat tgccattgag caggcccgct ttggcgacaa gctgacggtt
    23701 atctatgata ttgatgaaga ggtgaattgc tgcattccca gcctgctgat ccagccgttg
    23761 gtggagaacg ccattgtcca cggtattcag ccttgcaaag gtaaaggcgt tgtcaccatc
    23821 agcgttgcag agtgcggaaa tcgggtacgc attgcggtgc gagataccgg gcacggcatc
    23881 gttccaaagg tgattgagcg ggtcgaagcg aatgaaatgc cgggcaataa aattggcctg
    23941 ctgaatgtcc atcatcgcgt gaagttattg tatggcgagg ggctgcatat ccgccgcctg
    24001 gagccgggga cggaaattgc gttttacatt cctaaccaac gcaccccagt cgcctcacag
    24061 gctacgttat tgctttgagc cggagtgata ttgtgaaagt catcattgtt gaagacgaat
    24121 tcctggcaca acaggaactg agctggctaa ttaaagagca cagccagatg gagattgtcg
    24181 gcacctttga cgacggtctg gacgtgttga agtttttgca gcataaccgc gtcgacgcca
    24241 tttttctgga tatcaatatt ccgtcgctgg atggcgtgtt gctggcgcaa aacatcagcc
    24301 agttcgccca taaaccgttt attgtgttca tcaccgcgtg gaaagaacat gcggtagaag
    24361 cgtttgaact ggaggcgttt gactacattc tcaaaccgta tcaggagtca cgtattaccg
    24421 ggatgctgca aaaactggaa gcggcctggc aacaacagca gaccagcagt acgcctgccg
    24481 cgacggtaac gcgtgagaat gacaccatta atctggtgaa agatgagcga ataatcgtca
    24541 cgccaattaa cgatatctat tacgccgaag cgcacgagaa aatgaccttt gtctatacgc
    24601 ggcgtgaatc ctacgtaatg ccgatgaaca ttaccgaatt ttgcagcaaa ctgccgccgt
    24661 cgcatttttt ccgctgccat cgctcatttt gtgtcaatct gaacaaaata cgcgaaatcg
    24721 aaccgtggtt taataacacc tacattctgc gactgaaaga tctggatttt gaagtgccgg
    24781 tcagccgcag caaagtgaaa gaatttcgcc agttaatgca tctttaatcg ggaactttca
    24841 tgaaagcacc aggcttgccc gccgatcaac aatttttcgc cgatctgttc agcggcctag
    24901 tgcttaaccc gcaactactg gggcgcgtct ggtttgccag ccagcctgcc tcgttaccgg
    24961 tgggcagttt atgtattgat tttccccgtc tggatatcgt gctgcgcggc gaatacggca
    25021 acctgctgga agcaaagcag cagcgaatgg tggaaggaga aatgctgttt attccggcac
    25081 gcgcggctaa tttaccgatc aacaacaaac cggtgatgct gttaagcctg gtgttcgcac
    25141 cgacctggct tgggttatcg ttttacgata gccgcaccac gtcgttgttg catcctgctc
    25201 gccagaccca gcttcccagc ctgcaacgcg gtgaaggtga agcgatgctt accgccctca
    25261 cccatcttag ccgttcgccg ctggagcaga atatcattca gccactggtg ttaagtttgc
    25321 tgcatctttg ccgcaacgtg gtgaatatgc cgccgggtaa ttcacaaccg cgcggcgatt
    25381 ttctctatca cagcatttgt aactgggttc aggataatta tgcccagccg ctcacccgcg
    25441 agagcgtggc gcagtttttt aatatcacgc ccaatcatct gtcaaaactg tttgctcagc
    25501 atggaacgat gcgttttatc gagtatgtgc gttgggtgcg aatggcgaag gcgaggatga
    25561 ttttgcagaa atatcatctg tcaattcatg aagtggcaca gcgttgcggt tttccggata
    25621 gcgactattt ttgtcgcgtt ttccggcgtc agtttagtct gacgccggga gagtacagcg
    25681 cccgttttca gggctaacgt cagaaggtta attctgtttc cagcagcgtc aggatacttt
    25741 gtgcatcttg cgcggcaaac aacgactggc ggaagttttt attcaccagt ttacgcgcca
    25801 gctgcgagaa gactttcaca tgattcatcc cttcgttagc acccagcgtc agcatgatca
    25861 ccagttcgac ttcgcccatt tctgactgcc agtcaaccgg tttcgccagc cgggcaatgc
    25921 tgatactgga atgacggatc cactgagatt tggtgtgcgg gatcgctacg ccaaaaccaa
    25981 cgccggtggt aacaatctct tcccgctgcc agacatcttc ttccagctca aacggatgtt
    26041 cagtgcgccc gttaacgccg aggttgccgc acaggaactg gatcgcctgc tctttattgc
    26101 taaaatcctg atcaacaaag atattctcca gcgccagcag tgggcgaacg tcttcttccg
    26161 gcgtaaaggc ggtgagtaac gcttcaattt cctgcgcact gcggcattca catgcctgac
    26221 gcgccagttc ccgacacgcc tcgctatcca gttgacgaag ctggcttttc accgccggaa
    26281 tacgcgggct actcatactc agctcatcca ggcccagccc aagcagtagc ggcagataac
    26341 ggctttcacc gcccagttca ccgcaaatgc ctacccattt gccccgctga tgcgcagtgg
    26401 taactatttg ctgcaacatg cgcaggaacg atggcgtaat cgggttatat agcggcgata
    26461 cgcgcgggtt attacgatcg accgcataca gatactgggt catatcgttg gagccgatac
    26521 tgaagaaatc gacctcatcg cagaagtggt cgatgatgta gcacaccgac ggcacttcca
    26581 ccatgatccc aagcgtaatc gtctctgcat gacgcaggcc atcgcgctta agctcaacga
    26641 tcgctttttg gatctcgcct ttcacccata agatctgatc gaggctgtga accatcggga
    26701 tcatcaactg ggcgttgccg aaactggcgg cgcgcaaaat ggcccgcagt tgagtgcgga
    26761 acaggccagc aaattccggg taaatacgta ccgcgcgata gccgaggaac gggttctctt
    26821 cctggggaat attcagataa ggaatgcttt tatcgccgcc gatgtccatc gtgcggaaga
    26881 taatcggctt gtcgcccgcc gccagtagca cctgctggta ggcttcaaat tgctcctgct
    26941 cgtccggcgc gctgtcgcga tccatataga gcatttcggt acggaacagc ccgacacctt
    27001 ccgcgccgtt ggcaaacgcg cctggcgctt ccagagcggt gccgatattc gccgcaatat
    27061 caatacgctt gttatcacgg gaataggcca gctgcgcggc agcctgcgcc tgttgttttt
    27121 ggcgtttatc cgccagcgtc tgcgcgacct gataataacc gctcaccgcg tcattcgggt
    27181 taatcgccag cacgccgcac tgggcgtcaa gcactgcagg ttgcccggca taacgggcaa
    27241 tcgcatccag cggcaagcca ctcagtaccg ggatcgccga ggcacgggcc agaatcagtg
    27301 tatgcgaggt gcgcccggtt ttctccagaa tcatacccgc aagatttttc aaatcaaggc
    27361 tcaaaaactg gcttggggtt aaatcttcag ccaccagaat ggtcggtttt tccagcacca
    27421 gattgttgcg cggcttcagt tccggccagg tgatatgcag caactgttcg ctgatatcgc
    27481 gaatgtcgct gacccgctcg cgcagataat cgctggcaga agcagatagt ttggcgcaaa
    27541 cctgctccat attgctgatg atcgccgccc ccagtccctg atgctgttct gtcatcaggc
    27601 gacggatatt gcctgcaaat tcatcatcct gaatcagcga caaatgagcg ctgaggatag
    27661 ttttgctttc gccgtcacgc tcacgcaatt gctgattcag ttgctcggca agcgttgcca
    27721 ggctgtgctc cagccgggtg gaatcttgcg cactggcggg gattgcccga taactgtcga
    27781 ggctgtcgct ttgtaacagg gtcagcgtac ccacgccgac gccgcttgcc agcacattgc
    27841 cgtacagtaa atccgggtta aggcggctta atgaacgcgg cagcggatgc gccgtcagtt
    27901 ctgcctgcgt cggctgaacg ctgtcgctgt cgataaagcg caccaggatg tactcttcca
    27961 gcacgcgccg cgcctgctct tcatcgctgc cgctaatgtt caggctgcaa ctgtcattaa
    28021 ataaggtgcc agttccaatc aacgccagcg agcttttagc atcagccttt gcgttctggc
    28081 gatggttgat aaaggtgatt tcgctttgcc actggctgca ctgttcttta agttcccacg
    28141 ccggacgagc atgtagaccg ttaggcaaag gacagagaaa ttgaatcgtt aacataaccg
    28201 ctccgggtta atcaggagat catctgaaat ccgtcagttg aacaaccgtc tcacgcgtaa
    28261 gacgttgaat aagggcagat aaaagttgct ccatctgcaa aatgtcgcgg caatcgatgc
    28321 cgcgcaatgt ccgtggcggg tggcaggccc tattaccaat gtgggtacgc cagtaccggt
    28381 taagtgcacc gctccaccgt ccgtgccgcc gttactgaac atatccgcct gcaacggaac
    28441 gccaatttct gccgccacgg tttcaatcca cgcggtaagt tttggcggcg caatcagcga
    28501 tttatcgctt aacaccagca tcggcccgtt accaatctga cgatggttag ccgcgccata
    28561 atcaaagttt ttcgcccagc aggcggtatc aagcacaatg gcgacgtccg gcgacaccgc
    28621 gcgggtagca gtttgcccgc cgcgtaatcc cacctcttcg ctggaacttg ccaccagcca
    28681 cacttccgca ggcagttcgg cgtcgtgcag ctcacgtagt aacgtcacca gcagatagca
    28741 actgaggcga tcatcaaagg ctttgcccat cacccgctgg tgagggagga cctgaaaagt
    28801 ggtatcgaag gtgacgcgat cgcctggacg aattcccgcc tgcatcactt cgtcataggt
    28861 gcgcgcgcca atgtccacgc gcatggcgct aacgtcattc ccctgccggt cgccgtcaag
    28921 caggcctgga attttgcact cttcacgggt ggtgatgcgc accggctgca gctggcgggc
    28981 agccatgcgc acgttgccaa ccggcagcac gtcaatagcg ccttcgcggg agatgctgcg
    29041 caccataaat cccacttcgt ccatatgggc gcagatcatc accttcggac ctgtcgattc
    29101 attgaggcgg atcagcaccg atcccagacc atcaaatcgc acttcttttt gcaggcgagc
    29161 cgcttcttcc agcaggatct gccgcacttc ctgttccgag gaggcgatcg catctgcctc
    29221 gctcaacgct tttaatagcg ataaatccat tatgcctctc ccgtgagcaa cactgttttc
    29281 ggcatggcgt agagcacttc tgcgccttgc ggggtaacca gcacaacatc ttcgatgcgc
    29341 acgccccctt gccctagcaa ataaatcccc ggctccacgg tcagcaacat gcctggctgt
    29401 agcgtcgtgg tgtcccgcgg tgaaaaacgc ggatcttcat ggacttcaat gccgatagcg
    29461 tgaccagtgt tatgaccgaa atagtcgcca aatccagcct cggtaatgac ccggcgcgcg
    29521 gcgtcgtcaa tctgctggca gcgcacgccg gggcgaattg cagagattgc cgcgagctgt
    29581 gcctgcagga caatctgata gacgtcaaac agcgggtgag attcggcgct caccccttcg
    29641 ccattcacca gcaaggtgcg cgtcatatca gcagtagccc tgatacagcg caccaaaatc
    29701 gagagtgaca aactcgcccg ctgcaacaat cttgtcgctg gctttgccgt gcggcagcgc
    29761 cccgcgccag ccgctggcga caatggtgtc aaaagaggct ttttctgcgc cctgctggcg
    29821 cataaaccac tccagttcag cggctatctc gcgctcgctc atccccgcct gaataaagcg
    29881 gcgaatatgc tccgctccgc gatcggcaat cccacaggcg aggcggatta tctccacctc
    29941 ctctggcgtt ttgatttgcc gcagcacatc cggcgtggcg ctaaccagtt tcgcattgag
    30001 ttcagacttc cagcggtgcg cggtttccca actcacctgc tggctttcaa aaccgagcgt
    30061 ttgcaactgc tcatcggcaa tgatttgatt gacgatagtg gtaagcgtgt tcgtcgcgtc
    30121 aagcaaatgc agctggtagc cttgcgcacg ggcttccacc tcaacgaaat agcgcgaatc
    30181 taccagaatg tgcgcacttt cacggctaat aaccacatag cctgatccgg tggagatccc
    30241 cagatgcggc tgtttgttct gccgtgagga gagaagcacc gcatccagtt gttgcacctt
    30301 aagccagttg cgcagcgaag cgagtaatgt catctggatt ttccttctta caggctatcg
    30361 attaacaatt tgcctttgcg gaacatcatc agacgcagga acaccaccat cagcgcagta
    30421 atgattgccc ccagcgcgat ccccgccata taaacgccca ggttagtgac cagcggccat
    30481 gcccagatag cggattccgg gaaccattgc actgcgccca gccagacagc tgcggttgag
    30541 ccgacaatcg cgccgaccat atatgacgga atagcggtga tggggctttc cagcgcaaac
    30601 ggaatcgcac cctcactaat ccccataaag gccaggaaca tcgcggtctt accctgcgga
    30661 taaagttgag cgttgaacag gcgtttgccg gttaaacgac ggtcaataat ggtcgccaga
    30721 cccagaccaa tcggcggaat aacgatagcg atagaacgag cggtgaccgg taatacgtgg
    30781 tcagtggtaa agctgaaggc aacgaaacct gcggctttgt tgatcgggcc accaaggtcg
    30841 attgctgtcg ccgcggcgat ccccattgcg tacatcagcg cgcccttctc acctgcggca
    30901 gtcagcacag tacggatacc gccgttgatc cagccaccaa agggagtgat gacgtagtac
    30961 atcgccagca tgacaaaaat ggcggaaaga atcggtagca aaaatgtggt tttgaacgct
    31021 aacaggaaat ccggtaactg gattttctgg ttcatccatt tcacaaggta accggcaacg
    31081 atagaaataa tcagcgcacc aatgaaggtg gacggcaccg gcgaagaggt cgcccattgc
    31141 atagtgctgg gatcgaagtt cagcagttgc gtcggctggg tagacatcaa cccgccgata
    31201 aaaccagccg ggaacgccag tttgccaccg atagagttcg ccacaaatgc agcaaacatc
    31261 ggaatggcaa aaccaaacaa cacgccgccg aaggactgcg acagccaggc aaatttaagt
    31321 aaggagaggt cgaaaccgga gaatttcccg ctattaagag catccatgat gccgatctca
    31381 gcaggaattt taagccagct ataagcaatc agctggctga aagcgaggat cacaccgccc
    31441 ataattaagg tcggcaccat gcgtgaaatc cctgacatca catgctgcgg gagttcaccc
    31501 cagaagctgc ttttagaggc ctgcggattc ttaactgccg ctgccgcacc ggatgcgcca
    31561 ggcacaacgg ttgcactgcg ttttttaatg gccataacaa ttatccctgt cgaatattac
    31621 tgttgttcag aggcaatcat ctcttcgatt tctttgatga tgcccgcagc gtttttaatt
    31681 gcgtcctgca aagtgatttc ataaacgtcg cgtgattcga aacgttcgtt atcttccggg
    31741 gtaactgcca cggaatggat gatgatggtc gcttcggcga tatcctgcgc cgtcaggcga
    31801 ttctggatgc cgtccgcgcc ctgggtttca attttcactt cataaccggc ttctaccgcc
    31861 gcttcttcca gcacctgagc ggccataaaa gtgtgagcta ggcccattgg gcaggcacat
    31921 aaggcaatca gtttcttact catcgttaat tcctcgttca gtgatagttg acgagtgaaa
    31981 ttatgaacgt gcaaaacaaa tcgccgttac cagacaaata atgcatttac tggagaatta
    32041 atcccgctgt acggaagtgt gacgcaggtc ggccttgtga ggggacacaa agaaaaattg
    32101 aattaactgt atgatttaaa agattatcgg gagagttacc tcccgatata acaggaagga
    32161 tttacagaat gtgacctaag gtctggcgta aatgtgcacc ggaaccgaga agacccggat
    32221 agtcatggac gatgagatac accggaatat catggacata ttctttaaag cgccctttat
    32281 cttcaaatgc ggcacggaaa ccggaggctt tgaagaactc aaggaagcgc ggcacgatac
    32341 cgcccgcaat aaacacgccg ccaaatgtcc cgagattgag cgccagattg ccgccaaaac
    32401 ggcccataat gacgcaaaac agcgacaatg cgcggcggca atcggtgcag ctgtcagcca
    32461 gcgcgcgttc ggtaatatct tttggcttga gattttctgg caggcggttg tcagctttca
    32521 caattgcgcg atacaaattc accagcccag ggccagaaag cacgcgctcc gccgaaacat
    32581 gaccaatttc cgcacgcaat atttcgagga taatggcctc ttcttcacta ttcggcgcaa
    32641 aatcaacgtg accgccttcg cccggcaagc ttacccaacg cttatcgaca tggaccagat
    32701 gcgcaacccc aagccccgtt ccggcaccgt aaaccgcaat aggcttacct tcgaccggtt
    32761 ctgcgccacc aaactgaatc agatgctctt ttttcagcat cgggatcgcc atcgatacag
    32821 cggtaaaatc gttaataatt tccagatggc taaaaccgag attctttttc atttcggcaa
    32881 ttgagaacgc ccaggtatgg ttggtcattg ccacccagtc accggtaatt gggcaagcga
    32941 tggcaataca gccgtctttc acctcgacct tatgttcttc aagataaacg cgaatgaccg
    33001 cttcgaggct ggggtaatca agccctgaat aggtcttagc ctgcgagatt tcaccactgg
    33061 caatatcaca cagagcaaga cgtgcgttgg tgccgcccac atcaccgact aatgcatact
    33121 ttgtcattct tcaactgctc cgctaaagtc aaaataattc tttctcacac tgtaaatacc
    33181 tgggggcata acaacaacgc cggaaaggca ggctccctgt aaatatcgat ctgggtcaca
    33241 caattacttt atcgtttcag caccaattgc agcgatgcct ttttgcaagc tgggcaaact
    33301 aagtatctga ccccgcataa ggaatagaac atgctccatc cgcgagccag aaccatgttg
    33361 ttattatcgc tccccgccgt ggcaattggg attgcgtcca gtcttattct gattgtggtg
    33421 atgaaaatcg cctcggtatt acagaatttg ctctggcaac gactgccggg aactctgggg
    33481 atagcccagg attcacccct ctggatcatc ggtgtattaa cgctaacggg tattgcggtg
    33541 gggttggtta tccgtttcag ccagggtcat gccggaccag accccgcctg tgaaccgctg
    33601 atcggcgcac cggttccgcc ctctgcgcta cctggactta tcgtagcatt aattctcggt
    33661 cttgctggcg gcgtcagcct ggggccggaa catccgatca tgaccgtcaa tatcgccctt
    33721 gccgtcgcca ttggcgctcg tctgttaccg cgcgtcaacc gaatggagtg gactatttta
    33781 gcctctgccg gaaccatcgg cgcgctgttt ggtacacctg ttgcggcggc gttgatattt
    33841 tcgcaaacct taaatggcag tagtgaagtt ccgctatggg atcgtctttt tgcgccgtta
    33901 atggcggcag cagctggtgc acttactacc ggattatttt tccatcccca tttttcactg
    33961 cccattgcgc attacggaca gatggaaatg accgatattc tcagcggtgc aattgtcgcg
    34021 gcgattgcca tcgcagcagg gatggtcgcc gtatggtgct taccacggtt gcacgcgatg
    34081 atgaatcaaa tgaaaaatcc ggtgctcgtg ctgggtattg gcggatttat tctcggaatt
    34141 ctgggggtta ttggtggacc agtttcgctg tttaaagggc tggatgagat gcagcagatg
    34201 gtggcaaatc aggctttcag caccagcgat tactttttgc tggcggtaat taaacttgcc
    34261 gccctggtcg ttgctgccgc cagtggcttt cgcggtgggc gaatcttccc ggcagtgttt
    34321 gtcggcgtgg cattagggtt gatgctgcat gagcacgttc ccgccgttcc agcggcaata
    34381 accgtttcct gcgctattct cggcatcgtg ctggtggtaa cacgcgatgg ctggttaagt
    34441 ctttttatgg cggcagtcgt tgtacccaat accacattgc taccgctgct ctgtatcgtc
    34501 atgcttccgg catggctgtt attagcaggt aagccgatga tgatggtcaa tcgtccgaag
    34561 caacagccac cccacgataa tgtttagcaa aaaatgctct cctttatgat ttaagagtta
    34621 tggcgatgat gatggtcaat cgtccgaagc aacagccacc ccacgataat gtttagcaaa
    34681 aaatgctctc ctttatgatt taagagttat ggcgatgata cggggcttta cctccccgta
    34741 atattgcgtt aacaggccgc tgacggcttc gatcgtgaag gagaataaca atgttcaggt
    34801 cactgtttct ggcggccgcc ctgatggcat ttaccccgct tgcagcaaat gcaggtgaaa
    34861 tcaccctact gccatcgatc aaattacaaa ttggcgatcg cgatcattac ggtaattact
    34921 gggacggtgg tcactggcga gatcgtgatt actggcatcg caattatgaa tggcgcaaaa
    34981 accgctggtg gcgtcatgat aatggctatc atcgtggctg ggataagcgt aaagcatatg
    35041 agcgtggcta tcgtgaaggc tggcgcgatc gtgacgatca tcgcggaaaa ggccgcggac
    35101 atgggcaccg ccattaaacg cgtcctccaa tggagcacaa tgcctgatgc gacgctagcg
    35161 cgtcttatca ggcctacaaa ccggctcttt aacgcgtagg gcggataagg cgtttacgcc
    35221 gcatccgcca atagtgccag atgcgacgct cattcaacta caatcccagc gccgtcccca
    35281 ccaacaacca gatattcagc gccacgacca gcactacaat cacccagcct gtctgtttta
    35341 cgcgtttgct gttcaccaga tcgcccatca acttgctgtc actggtgaaa atcagcagtg
    35401 gaaccagcgc cagggcgata ccaaaactta acagcacctg actcataacc agaatccgtg
    35461 tcggatctaa tcccatcaga atgacaataa atgacggcaa catggtgact gtacgacgca
    35521 cccacagcgg gatatggaag cgaatgaatc cctgcatcac cacctgcccc gccagtgtcc
    35581 ccaccaccgt tgaggacagt ccggcagcaa ccagacttaa cccaaagacc gttgccgcag
    35641 catggcttaa cagcggttgc agcgtcagat aagcctcatc aagatcggca acaccagtat
    35701 gaccagaaaa gtggaacgcc gccgcagctg tagccatcat caccagattg acaaaaccgg
    35761 caatcgtcat ggcgatagcc acatcccatt tggtggcgga ataacgttgt tgacgcgaac
    35821 cgccatgtaa atgctgagtg agcgaagagt gcaaataaat cacatgcggc ataatcgtcg
    35881 cccctaacac gcctgctgcc aggaagaccg cctccgaagt aggtaaactc gggatcacca
    35941 ttcctttacc cagctgcgcc aggttaggct gggagaaaat caactcgaca atgtaagccg
    36001 cggcaacaaa caacagtaac ccgccaatca ctttctccag cggtttttgc ccgcgacgtt
    36061 gcagcattaa aatcaggaaa gtcgcgatcc ccgtcagcac cgcgccctgc aacaacgaaa
    36121 caccaagaat gagtttaaaa ccgatcgccg caccaataaa ttccgccagg tcggttgcca
    36181 tcgcaataat ttctgcctga acccaataga accacactac gggacgcgga tagtgatcac
    36241 gaatctgctc cgccagattt ttaccggtgg caatccctag tttggcagag aggatctgaa
    36301 tcagcatcgc catcaggttg gcccaaacga caacccacag tagctgatag ccgaagctag
    36361 cacccgcctg aatattggtc gcaaagttac cgggatcgat ataaccaatc gccgcaatga
    36421 acgcaggtcc cattaatgcg agcctcatct tgcgcgccgc ccgtccgctg ctactctcaa
    36481 cgcgatagtt cgtcatcttg tgcctctaaa acatagcctt tgctatgttt catgctatgc
    36541 taaacgagaa tgattatcaa attcatttaa atggattgtg gtgatttctc tgatagacca
    36601 agattatgat tacgaaaaga ttgatgactt gaatgttatg gttatgttta atgttagcac
    36661 atttacataa ctttcagctt ccatacacaa catagcagaa atgtatgaca gatcactatt
    36721 tttgaagcct gtcacaggac gtcattatag tgtgtgtcag atctcgtttt ccttaaccat
    36781 gttacataga atgtgcacgg aaatttaacc tgcctcatat ttggagcaaa tatggaccgc
    36841 gtccttcatt ttgtactggc acttgccgtt gttgtgattc tcgcactgct ggtaagcagc
    36901 gaccgcaaaa aaattcgtat ccgttatgtt attcaactgc ttgttatcga agtgttactg
    36961 gcgtggttct tcctgaactc cgacgttggt ttaggcttcg tgaaaggctt ctccgaaatg
    37021 ttcgaaaaac tgctcggatt tgccaacgaa gggactaact tcgtctttgg tagcatgaat
    37081 gatcaaggcc tggcattctt cttcctgaaa gtgctgtgcc caatcgtctt tatctctgca
    37141 ctgatcggta ttctccagca cattcgcgtg ttgccggtga ttatccgcgc aattggtttc
    37201 ctgctctcca aagtcaacgg tatgggcaaa ctggaatcct ttaacgccgt cagctccctg
    37261 attctgggtc agtcagaaaa ctttattgcc tataaagata tcctcggcaa aatctcccgt
    37321 aatcgtatgt acaccatggc tgccacggca atgtccaccg tgtcgatgtc catcgttggt
    37381 gcatacatga ccatgctgga accgaaatac gtcgttgctg cgctggtact gaacatgttc
    37441 agcaccttta tcgtgctgtc gctgatcaat ccttaccgtg ttgatgccag tgaagaaaac
    37501 atccagatgt ccaacctgca cgaaggtcag agcttcttcg aaatgctggg tgaatacatt
    37561 ctggcaggtt tcaaagttgc cattatcgtt gccgcgatgc tgatcggctt tatcgccctg
    37621 atcgccgcgc tgaacgcact gtttgccacc gttactggct ggtttggcta cagcatctcc
    37681 ttccagggca tcctgggcta catcttctat ccgattgcat gggtgatggg tgttccttcc
    37741 agtgaagcac tgcaagtggg cagtatcatg gcgaccaaac tggtttccaa cgagttcgtt
    37801 gcgatgatgg atctgcagaa aattgcttcc acgctctctc cgcgtgctga aggcatcatc
    37861 tctgtgttcc tagtttcctt cgctaacttc tcttcaatcg ggattatcgc aggtgcagtt
    37921 aaaggcctga atgaagagca aggtaacgtg gtttctcgct tcggtctgaa actggtttac
    37981 ggctctaccc tggtgagtgt gctgtctgcg tcaatcgcag cactggtgct gtaaaaccat
    38041 acataaaaaa gccggggatc attccccggc ttttttacgc ctgttaatca gctaatggct
    38101 gcgggcgacc aatcaaatac ccttgcagat attgcacccc gagcttatgc aatagcgcct
    38161 gctgctgttg cgtctcgaca aactccgcga ccacactcaa tgacttcgct ttcgccagat
    38221 cggtaattga tctcacaatc atcgcatcca gcgtattcgt gacaatatct ttcacaaaga
    38281 cgccatcaat tttgatgata tcagcctgca aacgctttaa ccgttcgtag ttggcatatc
    38341 cggtgccaaa gtcatcaatc gcaatccgga aaccaaactt atgcagctgc tcgatgttgt
    38401 acatgctgct ttctgcgtta gaaaacgcct gctcctcggt gatctcaaga atgaccgcct
    38461 gcggggagat gtgataacgc ttaaacagac gaataatccg cccggcaata ttcttttgca
    38521 gcagcgtgag cggcattaaa ttgactgaaa agcgcggtcc ttttttgtcg caagggtgtg
    38581 ttgccagcca cttcaacaag gattccagca cttgcaaatc aaaacgcgcg ctaaggttaa
    38641 actgagcaat aaggggcaga aacttatccg gggtcataat gccgccgtca tatttcagtc
    38701 gcgcgaggat ctcatcataa ccttcaccct ctttgttgcg aattggctgg gcgtagagca
    38761 gcaaatcacc ctgatctaac gccgtgcgaa tggtattcag caatagcacc tgtttagtgg
    38821 tctgcccgga aaccatctcc tctctgctat ccagcgccag cacatgatga tgtgcgcagg
    38881 attgctccgc cagccagctt aactgcccca acaagggttg cagggtttcc tgatttccat
    38941 caaaacgccc ccaggcagca ccatagccca tatccagccc ggtattgttc cagtgaattt
    39001 gccgactatt caggatgtta accatatgct ggagtcgccc ttccgtttcc ggcccgctca
    39061 gcactaacag cagttcacta cccggcaatt gatacaactt ttcgttttcc tgcatcaacg
    39121 gcagcagcgt acggcaaatt gagcggatac agtgaacgcg catcattaag ccgtaatgac
    39181 gactcataaa ctcaagatta tcaatgcgca ggcagcaaaa actcttgccc gcctcttgct
    39241 ccggcgcttg ttccaacgca cgaaagttgg gtaggagcgt taacggatct gttagcgcct
    39301 gcaaatgcca gcggcgatta agccattcac tgcgatgata aatgcgcacc atatagagca
    39361 ggcaaacgct aaaggaaatc agcaccgcga gaataaatgc cagcgaatat tcggtttcca
    39421 ccccttgcaa aaagttctgg ttgtaattca gaaggcaaag cgttgaaaca gcccaggtga
    39481 gatttaaaaa cggatagcga agattaccga ccccgagggt aaagatgatg aagaaaacgg
    39541 gtaccaggta accggcaata aagtcgtttt cataaggtgt gcacaacaga agtagcagca
    39601 cgcttaacgc tgccagccag cttaaggtaa atgcgagtga gatagtagaa aagcatgttg
    39661 taaatcagca ctgcggtgaa aaggcttagt aaatcaacga ccgtgaaaat ggcatccgca
    39721 tcgccgaaaa aggtggatat ctttagcggg aaatcaaaga agctccccac aagatacatg
    39781 ctgcatttga tgccgatcgg cgtcaccaaa ccaagccaga acagacgttg ccagacatat
    39841 cggctggtca atccataacg ccaacgcgtc cccagctgcc agcgtaatat tgcgcaggca
    39901 cacaacacga caaacgtctg gctgccgagc atgaccgcag tctgtaaaaa agagagatga
    39961 aagttccaca gattggtgca aaacatcccg cataaaacgg gaacgacacc gcgccagcca
    40021 aagataaaca agattgacag cattacgcac agtggcatcc acgccagaaa gatataactg
    40081 gaatggataa tggcaagtgg cgaaataaaa cgggatagct gaataagtac cacggtgagc
    40141 gtaaacgcta gtgtgaatat cttgatattt tttatcaggt tatgctccac aaacatgagt
    40201 acactactct cactgatcgg ggttttcagg cacacaaata ataagtttat tcgcatcgcg
    40261 aagcaaacag gggccagcct gttgccgatt tttcatccaa tgatgctttc accaggcggc
    40321 gctaacccgg acgtagactg tattacaaaa gcggcaaaaa gcagagacaa aaaacccccg
    40381 ctttgcagcg agggttggaa atttggtgga gctaagcggg atcgaaccgc tgacctcttg
    40441 catgccatgc aagcgctctc ccagctgagc tatagccccg tcacgtaaag cttgtcgagt
    40501 tgacgggcgg catcatatga attccgcccg aatgtgtcaa cggcaaattg caacgtgtag
    40561 tttcaatcgc tgaaaaatca ggcaaatgaa caattttgga aaaccgctcg cattcagtag
    40621 ttattcatgt cacggtttcc tgtaaagtgg tgttataaaa tgaactacta atagacccac
    40681 atacattcag ggaattgtta tgttcaagga gaggatgaca ccagatgaac ttgccagact
    40741 gaccggctat agccgccaga ccattaataa atgggtacgc aaggaaggct ggacaacgtc
    40801 accaaaacct ggcgtccagg gtggcaaagc ccgactggtt cacgtcaatg aacaggttcg
    40861 tgaatatatt cgcaatgctg aacgtccaga aggtcaggga gaagcgcctg ccctttctgg
    40921 tgatgcgccg cttgaagtat tgctggtgac actggcgaaa gagatgacgc cagttgaaca
    40981 aaaacagttt acatccttgc ttctgcggga agggattatc ggattgttac aacgcttagg
    41041 gattcgcgat agcaaataat atgaaaagat tacgcaataa aatgaccacc gaagaactgg
    41101 ctgaatgcct cggtgtggct aaacaaactg ttaaccgttg gaccagagaa aaaggctgga
    41161 aaacggaaaa atttcccggc gtgaaaggcg gtcgtgccag acttattctg gtcgatacgc
    41221 aagtttgcga gtttattcag aacacgccag ccttccataa cacaccaatg ttgatggaag
    41281 ctgaagagag aattgctgaa tacgctcctg gagcacgcgc cccggcgtat cgacaaatca
    41341 tcaacgcgat agataatatg acagacattg agcaggaaaa agtcgcgcaa tttttgtcac
    41401 gtgaaggaat tcgcaatttc ctcgcccgtc tcgatattga cgaatcagca taaataaaaa
    41461 acggcaggat attatctcct gccgtttatc tttttacacg ctaattactg ctgattttcg
    41521 cgttcagcaa taaaatccag cgctttgttg atacgctcga tactgcgggt cttaccaatt
    41581 gcgtgaacgg taacatccag tgctggagac tgccccgcac cggttacggc gacacgcagc
    41641 ggcataccaa ctttacccat acccacttcc agctcatccg ccgtcgcctg aatagcgtga
    41701 tgaacgtttt cagcggtcca gtcagtaatc gcggccagtt tgtcacgaac cacttccagc
    41761 ggctgacgcg ctaccggacg cagatgtttt ttcgccgcat cggcatcgaa ctcagcaaaa
    41821 tcttcgtaga aataacggca gctctgtgcc atctctttca gcgtcttgca gcgttcgccc
    41881 agcagtttca ccagatcagc cagctgcggg ccgttacggg tatcgatatt ttcctgctca
    41941 atgtgccact gtaagtgagt agcaacatac tccggcggca gcgcgttaat gtagtgatgg
    42001 ttcagccaca gcagcttgtc ggtgttgaac gcactggcag atttgctgac ggcattcaaa
    42061 gtgaagtatt tgatcatctc ttcacgagtg aagatttcct gatcgccgtg ggaccagccc
    42121 agacgcacca gatagttcag cagtgcttct ggcaaataac cgtcatcacg atactgcatt
    42181 acgctgactg ccccgtgacg tttggacagt tttttaccgt catcgccatt gatcatagaa
    42241 acgtgcgcgt aaaccggcac cggcgctttc agggccttaa gaatgttgat ctggcgcggc
    42301 gtgttgttga tatggtcttc gccacggata acgtgggtga tttccatatc ccagtcatcg
    42361 acaaccacac agaagttata ggttggggaa ccatcggtac ggcggataat aagatcgtcc
    42421 agttcctggt tgctgaactc gatcggacca cggatctgat cgtcaaaaac aacagaacct
    42481 tcctgcgggt tagcaaaacg tacaacacac ggttcatcat cagcatgatg ctcatggctg
    42541 tggcggcagc gaccgtcata acgcggcttc tcacctttcg ccatttgctc ttcgcgcagc
    42601 gcttccaggc gctctttaga gcaatagcat ttataagcag tgccctcttc cagcatctga
    42661 tcgatcaccg cgttgtagcg atcaaaacgt ttggtctggt agtacggacc ttcatcccac
    42721 tccaggctca gccagttcat gccatccata atggcttcga tagcttccgg cgtggaacgc
    42781 tcaagatcgg tgtcttcaat acgcagcacg aactcaccgc cgtggttacg tgcaaaaagc
    42841 caggagtaaa gagcagtacg cgcgccgcca acgtgcagat agcctgttgg gcttggcgcg
    42901 aagcgagttt tgattttcat gaaatggcct tacgtttaga aagatgccga caaccggcaa
    42961 atcctggaaa aattgagtgg gcgatattct atcactcccg cctgattcct caatgtagtt
    43021 cgggtttcta ccttacgcat tattgttttc gttgacaaat tgcgcaacac ggtctgtttt
    43081 gcgatcatct tgcttaaatt tacgacgaac gaacaatttc tttagaaaat gcgttgactc
    43141 attttgaact ctccctataa tgcgactcca cacagcgggg gtgattagct cagctgggag
    43201 agcacctccc ttacaaggag ggggtcggcg gttcgatccc gtcatcaccc accaactact
    43261 ttatgtagtc tccgccgtgt agcaagaaat tgagaagtgg gtgattagct cagctgggag
    43321 agcacctccc ttacaaggag ggggtcggcg gttcgatacc gtcatcaccc accacttcgg
    43381 gtcgttagct cagttggtag agcagttgac ttttaatcaa ttggtcgcag gttcgaatcc
    43441 tgcacgaccc accaatgtaa aaaagcgccc taaaggcgct tttttgctat ctgcgatact
    43501 caaagattcg aacctgcagc aggtttgagt tgagcgcagc gaaacaacgg agccgctcgc
    43561 ggcgacggcc cgaagggcga gcgaagcgag tcatcctgca cgacccacca atgcaaaaaa
    43621 gcgccctaaa ggcgcttttt tgctatctgc gatactcaaa gattcgaacc tgcagcaggt
    43681 ttgagttgag cgcagcgaaa caacggagcc gctcgcggcg acggcccgaa aggcgagcga
    43741 agcgagtcat cctgcacgac ccaccaatgc aaaaaagcgc cctaaaggcg cttttttgct
    43801 atctgcgatt tgtaagattg atttttcttc tcaagttcct tgttcttcta ccgatattac
    43861 ctcgtacagt gacgaggact gaacaatggc cactatctct tcaacttcaa ttccttccat
    43921 acaaacacag agtagtaaca gggcatcaca aggtagcgat gttgcttcac aaatcgcgcg
    43981 gataagtcag caaatcatca aactcactca acaaattaaa gagattgtag acactagcgg
    44041 cagtgctgaa gacaagcaaa aacaggccga attgattcaa cagcaaatca ccctactgga
    44101 aacacaactg gcgcagttgc agaaacagca ggcagaaaag gcgcaggaga aagagcagcg
    44161 tttgtctctg aatgtatccc ttctaaatcc tgttgaaaaa acgactcata tcgatatcta
    44221 tatctgatac aacggatccc cttcccgacc agccattagc gcattgccct ggtctcgtac
    44281 cacgtcccat aacgcttcag cggcggtgga aagtgaacgg ttcttcctgc gcaccagcat
    44341 caactggcgt tcaaccaccg gcgtaatgcg cttaaccacc agtgggctac cttcaggcaa
    44401 tggtagcgcc agtgcaggaa gaatactgat gccgatgcct gcggctacca tcgggaacag
    44461 cgtcgccgga tgtccaatct cctgcacaat attcgcctga ataccattgc gcgccagcgc
    44521 tgcgtcaatc agcggtcggc tgcctgacgc gtaatcctgc aacaccaatt tcgctccctg
    44581 taatgcctgc cagggtacat aatcttccac ggccaaagcg ctatcgcgat ggcaaagcag
    44641 aaagaaaggc tcggaaagaa tcgcttcaca ttgcagatcg cccactgggc ctggatcgat
    44701 gacgatgcca aagtcgacat ctccctggcg aatactttcc atcacccact gctgcggacg
    44761 atcatgcagg acaaactgaa tatctggata gcggcgatgg ctttcagcaa tgcattgcgg
    44821 aataagatgc gcggaaatgg tctggctggc agcgacccga acttttccgc tcagttgttg
    44881 ccccatacgc ccggtatcgc gcaacgtgct gttaagttca tccagcagtc gctcaagacg
    44941 caaagccaac tgctgccctg catctgtaag caccacttca cgcgtggttc tgtccagcag
    45001 gcgaacacca gtatgatttt ccagctcctt cacactgtga cttactgccg actggctcaa
    45061 gccgatacgc tctcctgcac gactaaaact tttctcctgc gctactgtga cgaaaacctt
    45121 taattgtttt aaagaataat tcatctgttt tcttcatgaa tggatgcaat aaatcaattt
    45181 tatttctcaa actggaagaa gcacaataga accatcgatc atctggagtc tttatgaaac
    45241 tttttcgtat cctcgatcct ttcaccttaa ccctgatcac ggtggtgttg ctggcctctt
    45301 tctttccggc cagaggcgat ttcgtcccct tctttgaaaa tctgaccacc gcagctattg
    45361 ccctgctgtt ctttatgcac ggcgcgaagt tgtcgcgtga ggcgattatt gctggcggtg
    45421 gtcactggcg actgcatttg tgggtaatgt gcagcacctt cgtgctgttt ccgattctgg
    45481 gtgtactgtt tgcctggtgg aaaccggtaa atgtcgaccc gatgctctac tccggttttc
    45541 tctacttgtg cattctcccg gctaccgtgc agtctgcaat cgccttcacg tcaatggcgg
    45601 gcggtaacgt cgcggcggcg gtttgttctg cgtcagcatc cagcctgctg gggattttcc
    45661 tttcaccatt gctggttggt ctggtgatga atgttcacgg tgcaaggggc agccttgagc
    45721 aggtcggtaa aattatgctg caactgctgc tgccgtttgt gttggggcat ctttcccggc
    45781 cgtggattgg tgactgggtg tcgcgcaata aaaaatggat tgcgaaaact gaccagacgt
    45841 ccattctgtt ggtggtttat acagcgttca gcgaagccgt cgttaatggt atctggcata
    45901 aagttggctg gggatcattg ctgtttatcg tggtggtcag ctgcgttctt ctggctatcg
    45961 tgattgtagt taacgtcttt atggcacgcc gactgagctt caataaggca gatgaaatta
    46021 ctatcgtctt ttgtggttcg aaaaagagtc tggcaaatgg catcccgatg gcaaacattc
    46081 tgttccccac atcggtgatc ggtatgatgg tgctgcccct gatgattttc catcagatcc
    46141 aattgatggt ctgtgcggtg ctggcgcgtc gatacaaacg ccagaccgaa cagttacagg
    46201 cgcagcagga aagcagcgcc gataaagctt aaagcggacg cttcaggggc tggaccagtt
    46261 gcgtcagccc ctcggtttta atcagcagcg tgatttgcat tagctcaccg agcttccctg
    46321 ccgggaactc atctttgcgg gcaaaccaca gcagatactc ctccggcagg tcaattaagc
    46381 gacgcccttt gtatttgcca aacggcatta tcgtattggc tatttcaatc agctgctctt
    46441 tttccatctc agctacccag caaacgcagc atttccgctt cgtcgatgac ttcaatgccc
    46501 agttcctgcg ccttcgccag tttagatcct gcagcttcac ccgctatcac cagatcggtt
    46561 ttcttcgaca cgctgcccgc gactttcgcg cccagttcga ccagtcgagc tttagcgtca
    46621 tcacgcgaca tctggcttaa gctgcccgta agcaccacgg ttttaccagc aaacgggctg
    46681 tcaatctctt ccgcgttgat aacgatcggc gcaggccagt gaacaccttc cgccaacagc
    46741 tcgctgatga cattgcggtt gctttcttcg gcaaagaagt tgtgaacgtg ggatgcaacg
    46801 acaatgccaa catcaggcac cttttgcagc tcttcaatcg aagcggcttc cagcgcttcc
    46861 agcgtgccga aatatgccgc cagacctgct gcggtggcct cgccgacttc acggatgcca
    46921 agtgcataga ggaagcgagc aaaggtggtt tctttcgctt tttccagcgc gttaaccacg
    46981 ttttgtgcca attttggccc catacgctcc agtccggtca gtttgcctgc ggtgagtttg
    47041 aacagatctg ccggagtatg gacatattct ttttcaacca gctgatcgat gattttgtcg
    47101 cccattccgt caacatccat cgcacggcgg gaaacaaagt gtttcagcga ctctttacgc
    47161 tgcgcaccgc aaatcaggcc accggtacag cgggcaaccg cttcaccttc cacacgctca
    47221 acgtcagaac cacataccgg acaatgcgtc gggaatacaa cctcacgggt atcttccggg
    47281 cgttcagaaa gcacgacgtt aaccacctgc gggatcacgt cgccagcgcg gcgaatcacc
    47341 actttatcgc caatgcgtaa accaagacgt tcgatttcat ccgcattgtg taaggttgcg
    47401 ttactcacca gcacgccagc aacatggaca ggttccagac gcgcaacagg cgtaatcgcg
    47461 ccagtacgcc caacctgaaa ctcgacgtca cgcacaaagg tcatctgctc ctgcgccggg
    47521 aatttaaacg caaccgccca gcgcggggca cgcgcgacaa agccaagctg ctcctgctgt
    47581 tccagtgagt tgaccttaat caccacgccg tcgatatcaa agcccagcgt cgggcggtct
    47641 tcttccactt tgtgatagaa cgccagcact tcttccgccg attcacaaag cgttacccga
    47701 tcgctgaccg gcacccccca ctttttaaat tgcagtaaac ggccaagatg agtatccggc
    47761 agctcgccac cttccagaac accaacgcca tagcagaaaa aagtgagcgg tcgcttcgct
    47821 gtaatacgcg gatcaagctg acgcagtgaa ccagctgccg cattacgtgg gttagcaaaa
    47881 actttcccgc ccgtgcgtcg cgcatcttcg ttaatctttt cgaaccccgc ctgcggcagg
    47941 aacacttcac cacgcacttc cagacgcgcc gggatattct ctccgtgcag cttcagcgga
    48001 atggcgcgaa tagtacgcac attagacgtg atatcttccc cggtggtgcc atcgccacgg
    48061 gtcgcggcac tgactaaaac gccattttca tacagaatac tgacggcaag gccatccagc
    48121 ttcagctcac agcaccaggt gactttctcg ttgtttttca gacggtcctg cacacgtttg
    48181 ttgaaagcaa gaaagctttc ttcatcaaaa acgttatcca gtgacagcat tggtacttca
    48241 tggcgtatct ggctgaaagc tgccagcggc gcagcaccta cacgttgggt aggcgaatca
    48301 ggcgtaatca gttctggatg tttggtttcc agctcgcgca gttcgcgcat cagcctgtcg
    48361 tattcagcgt cgggaatttc cggcgcatcc atcacatgat aaagatattc atgatggcaa
    48421 agcgtcgttc gcagttctgt cagttgttgt tcgattgatt ccatatcgca ccatcaatgc
    48481 taaaaacccc cgacaagcgg gggttcgaag aggagttaat ttgccttaag tgtatcaggc
    48541 gttggcgtct ttgacttcgc ggatgatgtc ctggtactcg cgcaatttct gcggagtcat
    48601 catacggcgc tgatcgtcaa gcacgacacc gcccacttca tcggcaatat gctgcgcaga
    48661 ttgcagcatc agcttgaagt tctgcagctc gtcaccgtaa gacggtacct gcataaagat
    48721 agtgacaccc ggagtagtga aatccttcat ttcaggatca aaggttcccg gtttcaccat
    48781 attcgccagg ctgaataacg ccgggccgct gccatccggg ctaagatgac gatggtaaat
    48841 attcatatcg ccaaaaatga agcccgcttg ttgaatgctg ttaagaagca gttcaccgtt
    48901 tagctcgcta ccgtgatgcg ccgcgacgtt catgataatc accgcttctt tgcgcttcgg
    48961 tttatccata actggagccg gttccgctac aggctcaggc tgtggtgccg ctacgggttc
    49021 tgcaggctgg aaagcctgtt gtgccggttg cggtgctgaa tgcacaggct gcggcgctgg
    49081 cgcgacctgt ggcgaaactg gctgctgcaa cggctgttca ggctgcggct gataggcagg
    49141 ctgctgcacc ggctgcgctg gacgcggagc atgttgcggc ggtacctgcg cttcaggcgg
    49201 ctgctggacc ggttgacgcg gctgcgcaga cgcataaggc ggtaggtact ggtgttgcgg
    49261 cgacggacga gcagcctcat gctcctgagc gttagccggg gcatgattca cgcggtgaac
    49321 acgaacctca ccaacgccct catcatcttc gacatcctcg tcataagaat cgtcgtcacg
    49381 ttttgacttc attcgtttta atggccgatc gcggaacata gaagatcgtt ctttacggct
    49441 ggtccagaaa ccatgtacca gtaaagcgat tatggcgatc gcgccaacaa tgattaatat
    49501 cagacgcaaa tcctgcatca ttatattctc tgttgttcta acaccttgcc accacggcaa
    49561 acatttactc actaagagta tttgccgatt acctcaagtg caagtgcact attaactttc
    49621 acagcacaaa gatagatgaa atcgtgcttt ttgctgtttt ttcgaacata tcctaactgt
    49681 ccattgcgca attacccggt aaaatacgca gaattttcct gggattggtc aaaaggagct
    49741 catcctgatt atggtttcat cattcacatc tgccccacgc agcggttttt actattttgc
    49801 gcaaggctgg aagctcgtct cgcaacctgg gattcggcgt ttcgttattt taccgctgct
    49861 ggtcaatatt ttgttgatgg ggggcgcatt ctggtggctc tttacacagc tcgatgtctg
    49921 gatcccgact ttcatgagtt acgttccgga ctggctacaa tggctgagtt atctgttgtg
    49981 gcctctggct gtcatctctg tgctgttagt gtttggctat ttcttctcca cgattgctaa
    50041 ctggattgcc gctccgttta acggtttatt ggctgaacaa ctggaagcac gattgactgg
    50101 cgctacaccg ccagataccg ggattttcgg tatcatgaaa gatgtgccgc gaatcatgaa
    50161 acgcgaatgg caaaaatttg tctggtatct gccgcgcgca attgtattac taattcttta
    50221 cttcatccct ggtattgggc aaaccgtcgc gccggtactg tggttcctgt ttagcgcctg
    50281 gatgttagcc atccagtatt gcgattaccc cttcgataac cacaaagtgc cgtttaaaga
    50341 gatgcgcacc gccctgcgca cacgcaaaat caccaatatg cagtttggtg ctttaaccag
    50401 cctgtttacg atgatcccgc tgcttaatct gttcatcatg cccgttgccg tttgtggcgc
    50461 gacggcgatg tgggtcgatt gctatcgcga taaacacgcg atgtggcggt aacaatctac
    50521 cggttatttt gtaaaccgtt tgtgtgaaac aggggtggct tatgccgccc cttattccat
    50581 cttgcatgtc attatttccc ttctgtatat agatatgcta aatccttact tccgcatatt
    50641 ctctgagcgg gtatgctacc tgttgtatcc caatttcata cagttaagga caggccatga
    50701 gtaagatttt tgaagataac tcgctgacta tcggtcacac gccgctggtt cgcctgaatc
    50761 gcatcggtaa cggacgcatt ctggcgaagg tggaatctcg taaccccagc ttcagcgtta
    50821 agtgccgtat cggtgccaac atgatttggg atgccgaaaa gcgcggcgtg ctgaaaccag
    50881 gcgttgaact ggttgaaccg accagcggta ataccgggat tgcactggcc tatgtagctg
    50941 ccgctcgcgg ttacaaactc accctgacca tgccagaaac catgagtatt gaacgccgca
    51001 agctgctgaa agcgttaggt gcaaacctgg tgctgacgga aggtgctaaa ggcatgaaag
    51061 gcgcaatcca aaaagcagaa gaaattgtcg ccagcaatcc agagaaatac ctgctgctgc
    51121 aacaattcag caatccggca aaccctgaaa ttcacgaaaa aaccaccggt ccggaaatct
    51181 gggaagatac cgacggtcag gttgatgtat ttattgctgg cgttgggact ggcggtacgc
    51241 tgactggcgt cagccgctac attaaaggca ccaaaggcaa gaccgatctt atctctgtcg
    51301 ccgttgagcc aaccgattct ccagttatcg cccaggtgct ggcaggtgaa gagattaaac
    51361 ctggcccgca taaaattcag ggtattggcg ctggttttat cccggctaac ctcgatctca
    51421 agctggtcga taaagtcatt ggcatcacca atgaagaagc catttctacc gcccgtcgtc
    51481 taatggaaga agaaggtatt cttgcaggta tctcttctgg agcagctgtt gccgcagcgt
    51541 tgaaactaca agaagatgaa agctttacca acaagaatat tgtggttatt ctaccatcat
    51601 cgggtgagcg ttatttaagc accgcattgt ttgccgatct cttcactgag aaagaattgc
    51661 aacagtaatg ccagcttgtt aaaaatgcgt aaaaaagcac ctttttaggt gcttttttgt
    51721 ggcctgcttc aaactttcgc ccctcctggc attgattcag cctgtcggaa ctggtattta
    51781 accagactaa ttattttgat gcgcgaaatt aatcgttaca ggaaaagcca aagctgaatc
    51841 gattttatga tttggttcaa ttcttccttt agcggcataa tgcttaatga cgtacgaaac
    51901 gtcagcggtc aacacccgcc agcaatggac tgtattgcgc tcttcgtgcg tcgcgtctgt
    51961 taaaaactgg cgctaacaat acaggctaaa gtcgaaccgc caggctagac tttagttcca
    52021 caacactaaa cctataagtt ggggaaatac aatgttccag caagaagtta ccattaccgc
    52081 tccgaacggt ctgcacaccc gccctgctgc ccagtttgta aaagaagcta agggcttcac
    52141 ttctgaaatt actgtgactt ccaacggcaa aagcgccagc gcgaaaagcc tgtttaaact
    52201 gcagactctg ggcctgactc aaggtaccgt tgtgactatc tccgcagaag gcgaagacga
    52261 gcagaaagcg gttgaacatc tggttaaact gatggcggaa ctcgagtaat ttcccgggtt
    52321 cttttaaaaa tcagtcacaa gtaaggtagg gttatgattt caggcatttt agcatccccg
    52381 ggtatcgctt tcggtaaagc tctgcttctg aaagaagacg aaattgtcat tgaccggaaa
    52441 aaaatttctg ccgaccaggt tgatcaggaa gttgaacgtt ttctgagcgg tcgtgccaag
    52501 gcatcagccc agctggaaac gatcaaaacg aaagctggtg aaacgttcgg tgaagaaaaa
    52561 gaagccatct ttgaagggca tattatgctg ctcgaagatg aggagctgga gcaggaaatc
    52621 atagccctga ttaaagataa acacatgaca gctgacgcag ctgctcatga agttatcgaa
    52681 ggtcaggctt ctgccctgga agagctggat gatgaatacc tgaaagaacg tgcggctgac
    52741 gtacgtgata tcggtaagcg cctgctgcgc aacatcctgg gcctgaagat tatcgacctg
    52801 agcgccattc aggatgaagt cattctggtt gccgctgacc tgacgccgtc cgaaaccgca
    52861 cagctgaacc tgaagaaggt gctgggtttc atcaccgacg cgggtggccg tacttcccac
    52921 acctctatca tggcgcgttc tctggaactg cctgctatcg tgggtaccgg tagcgtcacc
    52981 tctcaggtga aaaatgacga ctatctgatt ctggatgccg taaataatca ggtttacgtc
    53041 aatccaacca acgaagttat tgataaaatg cgcgctgttc aggagcaagt ggcttctgaa
    53101 aaagcagagc ttgctaaact gaaagatctg ccagctatta cgctggacgg tcaccaggta
    53161 gaagtatgcg ctaacattgg tacggttcgt gacgttgaag gtgcagagcg taacggcgct
    53221 gaaggcgttg gtctgtatcg tactgagttc ctgttcatgg accgcgacgc gctgcccact
    53281 gaagaagaac agtttgctgc ttacaaagca gtggctgaag cgtgtggctc gcaggcggtt
    53341 atcgttcgta ccatggacat cggcggcgac aaagagctgc catacatgaa cttcccgaaa
    53401 gaagagaacc cgttcctcgg ctggcgcgct atccgtatcg cgatggatcg taaagagatc
    53461 ctgcgcgatc agctccgcgc tatcctgcgt gcctcggctt tcggtaaatt gcgcattatg
    53521 ttcccgatga tcatctctgt tgaagaagtg cgtgcactgc gcaaagagat cgaaatctac
    53581 aaacaggaac tgcgcgacga aggtaaagcg tttgacgagt caattgaaat cggcgtaatg
    53641 gtggaaacac cggctgccgc aacaattgca cgtcatttag ccaaagaagt tgatttcttt
    53701 agtatcggca ccaatgattt aacgcagtac actctggcag ttgaccgtgg taatgatatg
    53761 atttcacacc tttaccagcc aatgtcaccg tccgtgctga acttgatcaa gcaagttatt
    53821 gatgcttctc atgctgaagg caaatggact ggcatgtgtg gtgagcttgc tggcgatgaa
    53881 cgtgctacac ttctgttgct ggggatgggt ctggacgaat tctctatgag cgccatttct
    53941 atcccgcgca ttaagaagat tatccgtaac acgaacttcg aagatgcgaa ggtgttagca
    54001 gagcaggctc ttgctcaacc gacaacggac gagttaatga cgctggttaa caagttcatt
    54061 gaagaaaaaa caatctgcta atccacgaga tgcggcccaa tttactgctt aggagaagat
    54121 catgggtttg ttcgataaac tgaaatctct ggtttccgac gacaaaaagg ataccggaac
    54181 tattgagatc attgctccgc tctctggcga gatcgtcaat atcgaagacg tgccggatgt
    54241 cgtttttgcg gaaaaaatcg ttggtgatgg tattgctatc aaaccaacgg gtaacaaaat
    54301 ggtcgcgcct gtagacggta ccattggtaa aatctttgaa accaatcacg cgttctctat
    54361 cgaatctgat agcggcgttg aactgttcgt ccacttcggt atcgacaccg ttgaactgaa
    54421 aggcgaaggc ttcaagcgta ttgctgaaga aggtcagcgc gtgaaagttg gcgatactgt
    54481 cattgaattt gatctgccgc tgctggaaga gaaagccaag tctaccctga ctccggttgt
    54541 tatctccaac atggacgaaa tcaaagaact gatcaaactg tccggtagcg taaccgtggg
    54601 tgaaaccccg gttatccgca tcaagaagta attcttgccg cagtgaaaaa tggcgcccat
    54661 cggcgccatt ttttatgctt ccgccagcgg cggcaaaatc aattcatcgc tctcatgctg
    54721 ctgggtgtag cgcatcactt ccagtacgcg caaccccgca cggtgcaccg catcgtttaa
    54781 cgccttccct ttcagcaaac cactgataag ctgagcacaa aacaggtcgc cagtcccttt
    54841 caggtcggtt tttacccgtg aatgggaaat gacattcacg ctgtcggcac tgaccaccac
    54901 cacctgcatc tcctgatttt cttcattacc ggaggcgctg gtaatcacca cccattttaa
    54961 tgtgtctgaa agcagacttt ttgcggcagc aatggcactg tcgagatcgc ggcaattttt
    55021 accggtcagg atttccagct caaagatatt gggggtaatt ccctgcgcca gcggcagtaa
    55081 atattgtcga tacgcctcgg gaaggtcagg tttgacataa attccgctat caatatcgcc
    55141 aatcaccgga tcgaccatga tcaataggtc aggatggtct ttgcgtagcg cagtcagcca
    55201 ctcggcaagg attttgattt gcgatgccgt tcccatatag cccgtggtta cagcacgaag
    55261 ttggcgcagc gcatcacgct cctgaagcgc acgcaaatag ccgctaaacc attcgtccgg
    55321 aatcgcacca ccgtagaaag tgtcataatg cggcgtattg ctcagcaata ccgtcggcac
    55381 ggcaaagaca ttcaagccgt tctgttttat agcaggcacc gcaatgctgt tgcctacgct
    55441 tccgtacact acctgcgact gcacggcgac gatatccgcc tgcagcgccc tactcttatc
    55501 gttaaacaac aacaaactac tcatttaatt ttttctcctt gccgatgatc ctcatcgtat
    55561 tccaaccgaa actttacctg attctggcag tcaaatcggc tattacaaaa caaagataag
    55621 gtaattcagt gaagaaaatc atttgtctgg ccattacgct gctaatgaca ctccccgctt
    55681 acgcgaagtt aactgttcat gaagaagccc gcatcaacgc catgctggag ggattagcac
    55741 agaaaaagga tttgatattt gtgcgcaacg gtgatgaaca tacctgcgat gaagcggttt
    55801 cccacctgcg tctgaagctc ggcaataccc gtaaccgcat tgacactgcc gagcagttta
    55861 ttgataaggt agcttcgtcg tcatcgatta ccgggaagcc gtatatcgtg aagatccccg
    55921 gtaagagcga tgagaacgca cagccttttt tacatgcgtt aattgcgcag acggataaaa
    55981 cggtgcctgc ggaaggaaat taatccgctt tggtaaggca tttacaggag gtaacatgaa
    56041 aaaatgcttt atttatcacg atgaaaaatc gaataaattt tggtggatag attacgaagg
    56101 ggatagttta gctgtcaact atggcaaggt aggtagtatt ggtaaattcc atacaaaaga
    56161 attcgataat gaagaacagt gtctgaaaga agccagtaaa ttgattgccg caaaaatgaa
    56221 aaaaggatat caagaaactc cagactttaa cttcatggat ctctactatt ttgatgatga
    56281 agagattggg ttacatctta aaacgtcaca cccaaacttc cagtgccatt ttactgatcc
    56341 actttatatg tgttgctggg atgaagaatc tccttttggt agcgatgaag gtgctgatgc
    56401 tctaaacgaa cttgaaaata gcctccgttc tggactgtgc tgatttccct caaatgttaa
    56461 ttgaaactat gtggggtatg aaatacatcg ctatggacag tattcttgaa gaggatgtgc
    56521 gtgcgcaatt actagccgat gaaatgagct ctatccagag caatatgatt acctacgcga
    56581 ctgcattcgg tcagatcaaa gtcatgggta aaatctccca taaacttaaa aagatgggac
    56641 tcaatgcact agcgcgtcat cagcttaccg caaaaattct tcaatggggt gacggtcagg
    56701 actcaccaat acttcaaaaa atgattgatg accttactgc ttttccacac gaaaattaaa
    56761 aactgcattt gtcggcagca acaactgccg acaaatcaga ttattgacaa agtgcacttt
    56821 gtttatgccg gatgcggcgt aaacgcctga tccggcataa aaatattgct aattcaatat
    56881 attgcagaaa cattgtaggc ctgataagcg gagcgcatca ggcagttttg cgtttgtcat
    56941 cagtctccaa tgctattaat ccttaaatcc ctgccccctg actaaaatgc tcttccccaa
    57001 acactccggt ggagaggtag cgatcgccac gatcgcagat gatcgctacc accaccgcac
    57061 cagggttagc ttttgccacc cgcagtgctc cagcaaccgc gccgcctgag ctgacgccac
    57121 agaatattcc ttcccgcacc gccagttcgc gcatggtgtt ttccgcatcg cgctgatgaa
    57181 tatccagcac ctcatccacc agcgcagcgt tgaaaatccc cggcagatat tccgcaggcc
    57241 agcggcgaat gcccggaata ctgctgcctt cttccggttg caggccaaca atggtcaccg
    57301 gtttggattg ttcgcgcata aagcatgaaa cgccggtgat agtgccggtc gtccccatgc
    57361 tggagacaaa atgagtgatg cgcccgccgg tttgctgcca gatttccggc ccggtggtgg
    57421 tgtaatgcgc ataagggtta tcgggattat tgaactgatc gagcagcttt ccttcgccac
    57481 gattcgccat ctccagcgcc agatcgcgcg caccttccat gccctgctct ttggtgacaa
    57541 gaatcagttc cgcaccataa gcacgcatcg ccgcacggcg ttcctggctc atgttgtcgg
    57601 gcatcagcaa tttcatgcga tagcctttca gcgcggcaat cattgccagc gcaatgccgg
    57661 tgttaccact ggtggcttcg attaagacat cacccggttt aatttccccg cgcttttccg
    57721 cctcgacgat catcgaaagt gccgcacgat ctttcaccga acctgccggg ttattgcctt
    57781 ccagttttaa ccacacttca ctgccgttat ccggccccat tcgctgcaac ttcaccagag
    57841 gcgtattgcc tattgtttgt tctaatgtac tcacaatctc tatccatacg tggtgttgcc
    57901 tgatgcgacg cttgcgcgtc ttatcaggcc tacaggttac aaaccttgcc attaaaaagc
    57961 ccggatcgcg ggaagcgcct ccgggcgttt aacattcact caacctatca ggcgctttgt
    58021 gcgagagcaa gttcctcatc gcgggtttca atacgctcgt cgccgttata cagccgcgca
    58081 tgttgcagac caacgaataa acgctcgcca tgctgcgggg catcgtcgcc atgcatcacg
    58141 accgtcagcg gttcgttgta ccaccccagc ggctgcacca ctaattgggt gtagtgacct
    58201 ttcgggctgg cttccagtac ctgtaccggc agcggcgaat cgagactggt acggcggctg
    58261 atatccactt cccacgggcg caggaagaga tccaccggcc cctgatacgc aggtgtgtag
    58321 cccagcggcc agcgatgcgc gccaacatgg aactgcccgc cgcgaatggt tccctgcagg
    58381 cggttcactt cgcccataaa ttcgagtaca aaacgggtcg ctggttcgcg ccatacctga
    58441 ttcggcgcat cagcctgttc aatattcccc tgactcatca ccactacacg atcggctact
    58501 tcggtcgcct cttcctggtc gtgggtcaca aacacgctgg tgaatttcag ctcttcatgc
    58561 agttgacgca gccagcgacg cagctcttta cgcacctgcg catccagcgc gccgaacggt
    58621 tcatcaagca gcagaatttg cggttctaca gccagcgcgc gcgccagcgc cacacgctgt
    58681 ttctggccgc cggaaagctg cgccggataa cgatccgcca gatgcgcaag ctggaccatt
    58741 tccagcaatt ttgtcacttt cgctttgatg gctgcggcat tcgggcgctc gcgacgcggc
    58801 agcaccgtca ggccaaaagt gatattgtcg aataccgtca tatggcggaa cagcgcgtaa
    58861 tgctggaaca cgaaaccgac tttacgatcg cgcgcgtgca ggcgactcac gtcggtgccg
    58921 tggaagcgaa tatgcccgct ggtttgatgc tccagcccgg cgataatccg cagcagcgtg
    58981 gttttcccgg aaccggacgg ccccagcaac gcgaccatct gaccagaagg aatatccagt
    59041 gagatatcgt tcagcacctg ggtgcgacca aaagacttct tgatattggc aatctcaatg
    59101 ctcatgatgc tcctcctgct gcgcgcgttt ttcctgattc tccaggcgcc actgcaacat
    59161 actctttaaa aacagggtga taatcgccat caacgtcaac agcgcggcgg cggtaaagga
    59221 gccgacggtg ttgtagtcct gctccaacaa ttcaatctgt aacggcagcg aaagtgtttc
    59281 gccgcgaatc gagccggaaa ccaccgacac tgcaccaaac tcgccaatcg cgcgggcgtt
    59341 ggtcagcacc acgccgtaaa gcagcgccca gcgaatattc ggcaatgtga cgcgacggaa
    59401 catctgccag ccggacgcgc caagcaaaat cgccgcttcg tcttcctggc tgccctggct
    59461 taacatcacc ggcaccagct cgcgcaccac aaacggacac gtcacaaaga tggtgaccag
    59521 caccattccg ggccaggaga acataatttg cagattatgt tcatccagcc agccgccgag
    59581 cgggccgttg gagccgtaga acagcagata caccagaccg gcaaccaccg gcgatacagc
    59641 aaacggaatg tccagtagcg tcagcagtaa ctggcgtccc gggaagttaa agcgcgtcac
    59701 cagccaggcc agcagaatgc cgaacaccag gtttaccggt acggcaatca gcgcgatcat
    59761 caccgtcagc cagatggcgt gcagcatgtc cggatcggcc agattctgta aaaccggcat
    59821 cagccccttg ctgaatgcct gcacgaagat gtaaatcatc ggcaccagca ggatgaacgc
    59881 cgaaaccagc atcccgatgc caatcagaaa ccatttgccc cagttaatcg ggcgcgcgtc
    59941 ataacgcttc aattgggtaa cttccgccat taatgaccta ccacacgccg accaaagcga
    60001 ctttgcagag tgttaattga gaacagcagc agcagagatg ccgcgaggat caccgaagca
    60061 atcgcgctcg ctgccgggta atcaaactcc tgtaagcgca caaaaatcat cagcgacgtc
    60121 acttccgtct tccacgcgat atttccggcg ataaaaatca ccgcgccaaa ttcaccaaga
    60181 ctacgggtaa acgacagcgc cacgcccgcc accagcgccg gagaaagctc cggcagcacc
    60241 actttgcaga aactctgcca gcgcgttgca ccaagcgttt ccgccgcttc ttcatattcc
    60301 gggcctaact cttccagcac cggctgcacg gtacgcacca caaacggaat gctggtaaag
    60361 gccatcgcca ccgcaatacc aagccaggta taggtgactt tgatatcaaa cttcgccagc
    60421 cattcaccgt aaaaaccgtt tacggaaaag agcgaggcca gcgttaaacc ggcgactgcc
    60481 gttggcagcg caaagggtaa atccatcagc gcatcaagca gcgtgcggcc cggaaagcga
    60541 taacgtgtta aaatccacgc catcagcaga ccgaaaacgc cgttaaaaat cgatgccaca
    60601 aacgccgaca gcagcgtcac tttgtaggcc gcgaccacct gcgggttggt gatcacctcc
    60661 cagtactgcg cccagctcat ctcagaaagc tgcatcacca gcgcagagag cggcagcagc
    60721 aatatcaggc acacaaacag cagactggtg ccaaggctta aagtaaagcc cggcagcacg
    60781 cgtcttgaag aaactgcaaa cattacttac gccccgccgc taacagcttg tctaactcgc
    60841 cgccgctggt gaagtgggtt ttcatcactt ccggccatga gccaaatttg tcttccacgc
    60901 ggaacagttc ggtctgcggg aatttatctt tcagtttgtc catgacttcc gggttattca
    60961 cacggtagta atagtcggtg atgatggtct gcgcctgtgg gctgtagagc cagttcaggt
    61021 aggcttttgc ggctttttcc gtaccgttgg cctgcacatt tttatcgacc cacgccaccg
    61081 ggaattccgc cagaatattg gttttcggga tcaccacttc aaagccctgt gcttcatact
    61141 gtttacggat gttgttcact tccgattcaa agctaatcag cacatcgccc aagccacgct
    61201 cggcaaaagt ggtggttgcg ccacgaccgc cggtatcgaa cacttcaacg tttttcagga
    61261 actgggtcat aaactgttcg gttttggctt tatcgccacc gtcagcttta tccgctgcgc
    61321 cccatgccgc cagataggta taacgcgcgt tacccgacgt tttcgggttc gggaaaatca
    61381 gcttcacgtc ggagcgcacc aggtcgttcc agtcgtggat attcttcggg ttgcccttac
    61441 gcaccaggaa gcccatggtg gagtagaacg gcgagctatt attcggcagg cgcgactgcc
    61501 agtcagccgg gatcagcttg cctttgtcgt gcaggatttg tacgtcggtc acctggttat
    61561 aagtgacaac gtctgctttt aagccctgca aaatcgccag cgcctgtttt gatgacccgg
    61621 catgagattg ttttatcgtc agtttgtcgc cgccgttatc ttttgcccat tgctgttcaa
    61681 acggcggatt cagggcggca aacagctcgc gggagacgtc ataagaactg ttcagcagtt
    61741 ccgttgcctg tacatggccc gccagcagca gagaagcgac cagcgcgagt gagttctttt
    61801 tcagtaagtt aacggccatt gcgcaccctt ataaatttaa tgactttcta atagccatca
    61861 tatttataac ggacgtgaaa ggagtaacgg ttttatatac cgtttggtga tttggaagtt
    61921 aaaaagggaa taagaacttg gttcaacagg cgtaattgtt gcaggcagtt tggacacgga
    61981 cagcgcggag aaaccggagc gtacacatag tacgtgagga tttcgagcac tgcccggggc
    62041 caaaatgaca aatgaaatag cctggtgaac ttagttcaag acgaaatcct ccccacacat
    62101 gcagggagga aacagaggtg aatcagatac cgacgctaac cgtctccggc agtgtgctgc
    62161 cgccatcaat cacattctgt gtaccggtta aatagctgga ttcatccgat gcgaggaagg
    62221 ccgccagttc gccgacttcc agcggatcgg cgaggcgaca cagcgggatt gcttttgcca
    62281 tttcagtcag caccgattct ggatcttccg ggttcgactg gcgggcaatg ctttccgcca
    62341 ttggcgtgcg gacgtatccc gggcagatgg cgttaacgcg aataccggat tgcgcgtact
    62401 ccaccgccag cgatttagtc aggccaacaa tcgccgcttt cgttaaggca tacgccgttt
    62461 cgccaggatc ggccaccata tcaccagtga ctgaagacat catcacaatg cgaccatctt
    62521 tgcgcgcaat catctccggc agcaccgcct tcgtgacgtt ccatacgcct ttaatattga
    62581 tatcaatatg gaaatcgcga tcttcatcgc tcatatcgag gaaactgccc agacgacaaa
    62641 cgcctgcgtt attcaccagg atatcaatgc gcccttcttt ttccttcgcg cgtttgatag
    62701 ctgcggctac cgacgccggg tcacgcacat cggcgacaac cgccgtacag cgatgaccac
    62761 gaccacacag ttcgtccgct agcttttcga tctcagggga gatatccagc aagattaggt
    62821 tcgcgccatg acgtgcaaaa gttctggcaa ttccttcgcc aattccctgc aatgcgcccg
    62881 taatcagtgc tgtcttgccc gtgagtttac ccattttaat gctctccttg ttaatacaga
    62941 gcgttacact ccattccctc tgaaaataca ggataaacaa aatattgctg cggatcagct
    63001 cactaattca ggaaaatctc aatccagtcg ctgctttatg catcatttca ggcgttgtgt
    63061 tagctcactg ctgcgctgaa tcattttcaa tgacgccaca tcattgatgt tgcacaagac
    63121 caacaaacag taaatcggta acagaatttt gcgccgtgcg ggtagacatc gacgagctac
    63181 gccattcggt ttcaccggaa acggtatcga gcgtgaaatg cgccagccgc cgcagcggag
    63241 agtcagccag agaagtaatg gcaatcaccg ttgccccctg ctttcgtgcc gcttctgcgc
    63301 acaagacaat ctctttttta ctgccgctgt aagagatagc gatttgtaca tcgccttttt
    63361 tcaacgcctg ggaaacggtg gcttgtacgt gcgtgtcggc ttcgcaggcc acgcgatagc
    63421 caattttcat cagtttgaac gacagatcgc gccccaccag cgccgatccg cccaggcccg
    63481 ttatctggat aaacggtgct ttactgatga cctcaatgat tttttgcagc cgcgcgtagt
    63541 cgaacaacgc acaggtttgc tccagcgcca gctctttttc gcgattcagt ttgcgggcaa
    63601 tcacttccag cgaatcatca ctggtaatcg aactgtgcag gtgcagcgcc gtggcattcg
    63661 ttttctcccg gctggcactg tattcgccaa ttaacgccat acgcaattca gtaaatcctt
    63721 gcgcgccgag cttctgggca aatttcacaa tgctcgactg gctgataccg agctgtttcg
    63781 ccatctgccg tgaagagacg gatttcagct cgctcactct ggcccgcaga aagtcagcaa
    63841 ttttttgctc gttcccggta aattcacttt ccgcattgcg tattttcgtc aggtacaaca
    63901 tcttccactc tcctgggctg gcagcaaaaa ttccgcttaa gttcttatga cgctcttcac
    63961 actctgcgag tgtagactca atgattcctt taagtctgtc aattcgggat atatgattcc
    64021 atacatctgc aattatttga tcgtaaatag taaggtcacc accgatgcaa ctagaaaaga
    64081 tgattactga aggctcgaac accgcctcgg ctgaaattga ccgcgtatcg acgctggaaa
    64141 tgtgccggat tatcaacgat gaagataaaa ccgtaccgcc tgccgttgat cgcgtcctgc
    64201 cggatatcgc cgcggcgatc gatgttatcc acgcccaggt cagcggcggc ggtcgtctga
    64261 tttatctcgg tgcgggaaca tccggtcgtc tggggattct ggatgccagc gaatgtccgc
    64321 ccacctacgg cgtgaaaccg ggtctggtgg ttggtttgat tgctggaggc gaatatgcca
    64381 ttcagcacgc ggtggaagga gctgaagata gccgggaagg tggtgttaac gatctgaaaa
    64441 atattaattt aacggcacag gatgtggtgg ttggcattgc tgccagcggt cgcacgccgt
    64501 atgtgattgc cggactggaa tacgcacgcc agctcggctg ccgcacagtg ggaatttcct
    64561 gtaatccggg gagcgccgtt tcaaccaccg ctgagtttgc cattacaccg attgtaggtg
    64621 ccgaagttgt taccggttct tcgcggatga aagcaggtac agcgcagaaa ctggtgctca
    64681 atatgctttc caccgggctg atgattaaat ccggcaaagt gttcggcaac ctgatggtcg
    64741 atgtggtcgc caccaacgaa aaactgcatg tgcgccaggt caatatcgtt aaaaacgcca
    64801 ccggatgtaa tgccgaacaa gcggaagcgg cgttaattgc ctgcgagcgc aactgtaaaa
    64861 cggccattgt gatggtgctg aaaaatctcg atgccgcaga agctaaaaaa cgcctggatc
    64921 aacacggcgg ctttattcgt caggttttag acaaggaata acccatggcc aaagagatca
    64981 gcagtgaact tctgaacacc attcttaccc gtgtcggcgg accgggaaat atcgccagtt
    65041 gtggtaactg tatgacgcgc ctgcgtctgg gtgtacatga cagttcactg gttgatccca
    65101 atatcaaaac gctggaaggc gtgaagggcg tcattttgac cagcgaccag gtgcaggtcg
    65161 tttttgggcc tggtaaagcg catcgtgccg caaaagcgat gagcgagctg ctgggcgatg
    65221 caccggtaca ggatgccgca gaaatcgccg cccagaacaa acgtcagtta aaagccaaac
    65281 aaacctccgg cgtgcaacaa tttctcgcca aattcgccac catcttcacg ccgctgatcc
    65341 ccggttttat tgccgccggt ctgctgctgg ggatagcgac gttaattgcc acggtgatgc
    65401 acgttccggc agatgctcag ggcacgctac ccgacgcgct gaattttatg aaggtgttca
    65461 gcaaaggttt gttcaccttc ctagtgattc tggtgggcta taacgccgcc caggcatttg
    65521 gcggcacggg cgtcaatggc gcaattattg ccgcactctt tttgctcggt tataaccccg
    65581 ctgcaaccac cggttactac gccggttttc acgatttctt tggtctgccc atcgatccgc
    65641 gcggcaatat tatcggcgtg ctgattgccg cgtgggcctg cgcccgcatt gaaggcatgg
    65701 tgcgccgctt tatgccggac gatctcgaca tgctgctgac ctcgttaatc accctgctga
    65761 tcaccgctac gcttgcgtac ctgatcatta tgccgctggg tggctggtta ttcgaaggta
    65821 tgtcatggct gtttatgcac ctgaacagta atccgttcgg ttgtgcggtt ttagccgggc
    65881 tgttcctgat cgccgtggta tttggcgtgc atcagggctt tattcctgtt tacctcgcgt
    65941 taatggacag ccagggattc aacagcttat ttcccatcct ttcaatggca ggcgcgggcc
    66001 aggtgggcgc ggcgctggca ctctactggc gggcgcaacc gcacagtgcg ctacgcagtc
    66061 aggtacgcgg ggcgattatt cccggcctgc tgggcgttgg tgaaccgctg atttacggtg
    66121 tcaccctgcc ccgcatgaag ccgtttgtta ccgcctgttt aggcggcgcg gcgggcggtt
    66181 tgtttatcgg attgatagcc tggtggggtc tgccgatggg cttaaacagc gcctttgggc
    66241 cgtctggtct ggtggcgctg ccgctgatga cttccgcaca aggcatcctt ccggcaatgg
    66301 cggtttatgc tggcgggatt ctggtggcat gggtttgcgg gtttattttc accacgctct
    66361 ttggctgccg taacgtcaat ctggactgat attatgaaac ggacaatgct ctatctttct
    66421 ctgctggctg ttagttgtag cgtcagcgcc gcaaaatacc ctgttctgac agaaggtagt
    66481 tgatcaagta tatgcggcgt taaagcagta ataaaaaggc cagtcagtaa tgttactgcc
    66541 tggcctttaa tcaatgaatc agaaacgctt acagcgccat caacttgtcc agcgacggtg
    66601 cgaaataata gccgccggtt accggtttgg tgaaacgcaa catcgcatca cgtttgccat
    66661 cggtatcgcc aaacatgctc agcagttgct gctcaatgtt atgcagacgc gcgcagtagg
    66721 cgcagaagta cagaccgtga gtgccactgg cggtgccgta cggcaggctc tggcgaacaa
    66781 tcttcagccc tttgccatct tctttcagat caacgcgggt gaggtgagag gtttccggac
    66841 gttcgtcgcc gtcaatctct tcgttggcct ctttggtgcg cccgatcatc atctcctgat
    66901 cgtgaacgct catccggttg agctgcttca ggttgtgctc ccaacgctgc acaaacacat
    66961 agctgccgcc ggaatccacg ccgtctttga taaccgccac ttcgcggcgc gtctcttcac
    67021 ccgccgggtt ttctgtaccg tcaacaaagc cgctcaggtc acgctcttcc acccaacgga
    67081 agccgtggat ctcttctttc acttcaatgc agtcaccaaa ggcttccatc gccgcctgag
    67141 cgacagagaa gtttacgtcg tgacgcagag aaagaatgtg gatcaacaca tcgaactggg
    67201 tcgtcggcgc aaggccttta ccgtagcccg gaaaatcttt cagctcttct gccccaacgc
    67261 cgccgctcag agcgcgccag gtgttgttac caaaggcaac caccgcacca agatgcgcgt
    67321 ccgggaattt cgcttcaaaa gttgccagtt tgtcggcaaa tgttttactg gccgcacgca
    67381 gggcgtcaac ttcccctttc acgttggctt cgatccaaat cgccgcgcgg caatgttctg
    67441 gcaaaatgcc actctgaacc tgagacattg ttcctcctga aaataagaat gccacgcaat
    67501 ccgtggcatt gttgagcgct attttacgtg ttttttcgcg acggcggttt gttcagacgc
    67561 aaattaacgc cgccagataa ttttactgac tttccagttt ttcagggtat cgtccgaagg
    67621 cattaaccct tcaggaccac gccattcccc gctgaactgg tagctaatat gttgactgcc
    67681 ttcggcttta cattcgacgg cacgattatc atcaccatca gctttttggc aattgccaaa
    67741 tgctttgctg taaaggtcgc taaacggtgt accgatttta acaccggtgt cagcaggaat
    67801 atcgctatcc agcacgtcaa tgcgactgat cgtgccttga tcgccgttaa tcaccatcgc
    67861 cacgttgtcg cctttcatca cttcaaaaaa gcgcaccacg ttgccgttcg cggttttcat
    67921 tccgctgcgc aggcgataat cgccatcaag cgcatcagca atggcttgtt cttgcagtgg
    67981 tgtggacgcc gttaattcac ccacgccctg ctcgctcact ttggtggacg atccaaacca
    68041 gttccacggg ttagcggcag accagttaac tgacgacatc gtggaacagc cggtcagcat
    68101 caacggcata gcgcataaca ttaaacgcag cgatttcatg tcacttcctt tggttattca
    68161 ataacgttgc ttggagtgca aattcaccaa aaagtgccgt tattcttttt cttcattaaa
    68221 acaagcgcgt aaacgtcgat tggtcagcag ccagattaac gccacaatat ccgccactac
    68281 cagcgccaga ccgataccgc taacggattc accgttcagc cacagccacg gttgccagca
    68341 aagtaaaacc acctgcgcca acagcaacag aaaatagagc acatgccagg tgcgaggaaa
    68401 cgaagcccgc cgaccgctca acaaaaacgc cagcaccgcc ggaataccgg gaatcagccc
    68461 cagccagaaa ttatcgtgat cgggataaaa cagatttagc agcgcagtac cctgctcacg
    68521 cgacgcaccg gcaatgacaa acagcaccca ggttcgcgcc tgaagcaata gcacaagcca
    68581 gaagagcaag ggtaaacgca ggcgtccgtg cgcatcataa tggacaggat ggaactcagt
    68641 actcttcatc ttcaatcaaa cgcttaccca gactcagcac gtcggcgtgt tcatatccca
    68701 ggcgttcata cattccgagc accatgtcgt tatcttccgg cacgttgatc tgaattttcg
    68761 ggcagccgcg agcaatcagc tttttctcca gccggttaag caacgcattg gcaattccac
    68821 gcccacgaaa ctctggatgc acgccaagat aataagcaga cccgcgatgc ccgtcataac
    68881 cgcccatcac cgttccgacc acttcaccgt ttacctcagc gaccaaaaac aaactgacgt
    68941 catggttcat cttacgctcg atgtccattt ccggatcgtt ccacggacgc agcaagtcgc
    69001 aacgctccca aagggtgatg acctcttcga aatcttcctg gcgaaatacg cgtatctcca
    69061 tggtattcgt tacctttttg cgggttaaaa ggctgattat ggcgtgaacg gtcgaattag
    69121 ccaatatctg acgaaaatcg gttgaaaaag tggcataatg gggagttgtc aactattgaa
    69181 atgaaaagta aaacaattct caacagcaaa ccgtcgtaac ggattacgcg atacgatata
    69241 acatctggaa ctttattatt acaactcagg ccgtatgagc acttttaaac cactaaaaac
    69301 actcacttcg cgccgccagg tgctgaaagc cggtttagct gccctgacgt tgtcaggaat
    69361 gtcgcaagcc attgccaaag acgaaccttt aaaaaccagc aacggacaca gcaagccgaa
    69421 agccaaaaaa tctggcggca aacgtgtcgt tgttctcgat ccaggccacg gcggaattga
    69481 taccggagcg atcggacgca acggttcgaa agaaaaacat gtggtgctgg cgattgctaa
    69541 aaacgtccgt tccattttgc gtaatcatgg gattgatgcg cgtttaacgc gttctggcga
    69601 tacgtttatc ccactttacg atcgcgttga aatcgcccat aaacatggcg cagatctgtt
    69661 tatgtcaatt catgccgatg gctttaccaa cccgaaagct gccggtgctt cggtatttgc
    69721 cctctctaac cgtggggcaa gtagcgcaat ggcgaaatac ctgtctgaac gcgaaaaccg
    69781 cgccgatgaa gttgccggta aaaaggcgac tgacaaggat cacctattgc agcaagtact
    69841 gtttgatctg gtgcaaaccg atactattaa aaacagcctg acgcttggct cgcatattct
    69901 gaaaaagatt aagccggtgc ataaactgca cagccgcaac accgaacaag ccgcgtttgt
    69961 ggtgttgaaa tcgccgtcgg ttccttcggt gctggtggaa acctcgttta tcaccaaccc
    70021 ggaagaagag cggctgttag gcacggcggc gtttcgtcag aaaatcgcca cagcgattgc
    70081 tgaaggcgtg atcagttatt tccactggtt cgacaaccag aaagcacatt cgaaaaagcg
    70141 ataagttatg aaacccgacg cacaccaggt taaacagttt ctgctcaacc ttcaggatac
    70201 gatttgtcag cagctgaccg ccgtcgatgg cgcagaattt gtcgaagata gttggcagcg
    70261 cgaagctggc ggcggcgggc gcagtcgggt gttgcgtaat ggtggtgttt tcgaacaggc
    70321 gggcgtcaac ttttctcacg tccacggtga ggcgatgcct gcttccgcca ccgctcatcg
    70381 cccggaactt gccgggcgca gtttcgaggc gatgggcgtt tcactggtag tgcatccgca
    70441 taacccgtat gttcccacca gccacgcgaa tgtgcgtttt tttattgccg aaaaaccggg
    70501 tgcagagccc gtttggtggt ttggcggcgg cttcgattta acccctttct atggttttga
    70561 agaagacgcc attcactggc accgcaccgc ccgtgacctg tgccagccat ttggtgaaga
    70621 cgtttatccc cgttacaaaa agtggtgcga cgattacttc tacctcaaac atcgcaacga
    70681 acagcgcggc attggcgggc tgttctttga tgatctgaac acgccagatt tcgaccactg
    70741 ttttgccttt atgcaggcgg taggcaaagg ctacaccgac gcttatttac caattgtaga
    70801 gcgacgtaaa gcgatggcct acggcgagcg cgagcgcaat tttcagctct accgtcgcgg
    70861 tcgttatgtc gagttcaatc tggtctggga tcgcggcacg ctgtttggcc tgcaaactgg
    70921 cgggcgcacc gagtcgatcc tgatgtcaat gccgccactg gtacgctggg agtatgatta
    70981 tcagccaaaa gatggcagcc cagaagcggc gttaagtgag tttattaagg tcagggattg
    71041 ggtgtaactc cctcaccccc attcccgcat ccgctgatgc aacgtcaacg actgcttctc
    71101 ggcaaacagc tgctggtaat ccgtggcaaa ttgccccaga tgccagaatc cccactgcat
    71161 ggcggcgtct tttaccgttg tgctttgcga ccacgggctt atcagttcgc ggcgtacggc
    71221 gttcaggcga atgcgtttca gccacgcgtt tgggccaatg cctaaaatag cgtgaaacgc
    71281 gttttgtagc gtgcggcgac tgacatgcag ttgattacac aaatccagca ccgtcaccgg
    71341 ttcggacatg ttttccagca catattcacg ggcgcgggaa agcaaccgac ggtaactctg
    71401 atgactgatg ctttccgccg tcaccattgg ttgcgcttct tccagcatcg cccccatcgc
    71461 cattagcaaa ttatccccca gcacttttcg cactgcaggc tgatggagat tttccgggtt
    71521 ctcgcaaaac gtcgccagcg cctgttggac aaagccccac agcgcggctt tatgctgctc
    71581 tttcacttcc agcgccgact ggctacgcag catatgtaat acccgatccg gattgtgcaa
    71641 aaagttagcc tgacaggtga tgacatcttc agacagcact acgcccagga tcgtataatc
    71701 gtccggcgta cttaattcaa actcggttcc accggggcgc gtcgctattt ctgcgcttcc
    71761 cagacattgc gaaccgataa atccctgctc accgcgcgtc gccggaatgc caaaccagaa
    71821 cgagttcggc cagaccaggc acgactgacg cagcgccaga ccggtgtatt cacgaaaaac
    71881 ctgaatatca tcgagtagaa tttccgtaaa ttcaccatga aacttgcccg gatgcagctg
    71941 atcgtaaatc tgctgctagg cggtaatcgt caaagcatgt tcatagacat ccgttgtctg
    72001 tcgttgatga acattatcca cttcgacctt cggcgtgagc ttcaggtttt cgggtaaggg
    72061 ttcatgataa agatggtgca aattggctgt acgggtcttt ttcatgatgt taatgccggg
    72121 tgttgtagga cacccggcac ctccgacagg ttaatggggc ttgagacgat aacgactact
    72181 gcgtttacgt aacgtcccgg cagaaaagag ctgttcgagc gcatttctgg ctttttccag
    72241 cggccagcca aagtgggcgg ccacttctcc tgccgtcatt ccctgacata ctgatgttaa
    72301 cagcgccaac aactcgtcag cagattccgc gacgataact ggcgcgtcgg gttcctttac
    72361 gggttgcttc ggctggcggt taaagccagt aaccagccac tgggtgtcat cgtctggccg
    72421 accgatctcc ttacggctga tgacacgccc ggtacgcatc gcggcagcac aacctgcgtc
    72481 cagcgccgca cgacacgccg ccagatcgcc ttccaccacc agcgttaagc gaccagggtc
    72541 aagcacttcg tgactgagca gacgaacgtt agctgctttg agcatggcat ccgctgcatc
    72601 tatcgcggcg accatgccgt ccacttccag caatcccagt gcattgatca tcggcaacct
    72661 ccgttacgca cgctggattg gattacgcgc gatttccagc actgcatcgg taaaggcgtt
    72721 acaggctgct ttacacgcgg cctggctacc ggttaaaaat gctgccgagt agttggtttc
    72781 agacggtggc gggacgtagg tcaccagctg tacgtcggca gatttcaacg cggcatcaat
    72841 gccgtaggtc gcttccagcg gcggtgccac cagatacgcc atcggatcgc caagcgtgat
    72901 cccggcggtt gatgaaagat aagaaccggt acgcgaaacc acatgcgcca ggaaggcggt
    72961 gttttctgcg tcattggccc actggaaagc tgcgccattt tcaatatgtg caaccatcgc
    73021 atccagaccg gcacgcactt ccgccgggtt tggcccacca agcataatca gcacttcacc
    73081 ggcagtcggt gacgggccgt gtgccgctcc cgcatacagc gagcgaccat ataccacttc
    73141 gaccatcgct tgtttggtcg cttcgtcagc cgcaatgtag gttacgtcgt cagaatcggc
    73201 agaaatcagc ccgaggctac gaatatgcgg cggcaatttt agttcacgcg caaattcagc
    73261 gttaacggag gcaatcaccc gcatggcggt taccgacggt cgaatcaaat ctaaagctgg
    73321 catgatgcct ccttatcggg tcatgttgat gccggatgct ttctgctcca gcatacgttt
    73381 cgccaaatcc acaatgacgg cacagcagca cgaaagagaa gcaaaagtca gctacctggt
    73441 acactaggca gcgggtcagc aaaggggggc aaatgcccct tttttcatgt cagataatca
    73501 gatcattacc tcaataaact aataatcaca tcgcggttac aaccagtttg atagtgatag
    73561 ctcaaaccca gacgtggcgc tggctgcaag caaaataagc cgcaaaaatg cgaccccatg
    73621 tttactagcc gcaaaaatgc ggtcctattt gccagaacct tttattattc aattggttag
    73681 tgcaagaaaa agcgtgatta ctgttcaaga agtatccaga aaggtcatga ttttgacgct
    73741 gaaaaaaaat aggtgggcat ttttgcccac tcactagccg caaatttgcc cactcactag
    73801 ccgcattttt gcggcccaaa gtaataaata cagagaaata ataaatacag atcagcgcca
    73861 agatttttcg tcttttcggc ggaatcatga gactagtgct tacgtcctga taacctccag
    73921 agcagacctt aacttctgtg cagcaggact acgctcattg ccgctcggct aggtcaaatc
    73981 ctcacgataa agctgttcgg attgtgacca taatgttaaa ggactcttta aatcattcct
    74041 tgacgaatta actagctggc ttaagcggaa tagcatgcag gataaaaaca aaaccagctt
    74101 agcagtgggg agagcttcgc ttcgctcgtc tcctggggcc aatgctcaat gaaacgacgt
    74161 aaggcacgga gcgctcacca taggtagaca cataaacatc tgagagcgca ccttctatcg
    74221 cgctacgcgc tcaataaaaa ccaacgacac cgccacactg caagaccaat ggtttttatc
    74281 gcgtatacag accagacagg aaacagcagc aaagcagcgt atagccaggt gcagacagca
    74341 cactcacacg acgcacagca ggcttaagtg atcactcagg aaactttatc cccgccgccg
    74401 atcaccgcga actatcatga ccgcgccaaa ttcgaaagga atggcccata gaacgcgatg
    74461 aacacacata agcgctacac ttacttaacg tcgccacaga gcgattgaga gcgcttaaaa
    74521 tcgatgcgat ttcattaaat ttttctcact cgcctgaatg gtggcgatca ggactgatcg
    74581 caacattgcc ccgcctcgat attgagcgca taatagatac atacattaag cggaaaagag
    74641 ataagtttat tgtgaaaatc agggggttac atatcatgcg catatcagaa caacaacggc
    74701 agatcctcga ctatctggca aaaaacgaat caatcaccgc acgacaagcc gcagatctcg
    74761 tttatggtgg caatgttacc cgcgtccagg ttgacgccgc ccgccgctca ctcatgacga
    74821 tggttagtaa tgggctaatc aggaaacaag gccgcgcttt cgtagctgaa agacatatca
    74881 atcacgctta tagccaggac gttgaagaat tgcgccgcca gatcctttgg gtattcagca
    74941 aacagggtca ggagtatttc ctttctgaac acccaaattt cgccgccaaa gagttaacag
    75001 taagcggatt gtcgtgcgca ctggattatc ttgtcggtga aggttgcgtt acttacaagc
    75061 agctatggca cgccctgaat acgcttgtag aagatggttt actcattgcc acgcgtgaga
    75121 acatcgaata ctggataatg ccgcgttcag gtcgccgcaa tcatgcacgg tatatttcag
    75181 gcaacagata cagaacgccg gactttaatc cgacacagga agatcgggac cgtgccgaaa
    75241 tgatgtgcag ggaacaggag aaaagaatta tggataagtt attttcgatg cagtgaacac
    75301 aagcgggcgc aatgcccgtt tttattgcct tattccacgc ctgacgaatc aatttgatca
    75361 cacctgagaa aatgcatagc cagaagttaa ggccgttata ttgcgattgg gagcgtttta
    75421 aacggtatca atttaattaa ttctgatgtg gtgatttgca ggcaaaaaaa acccgccgga
    75481 gaagcgggaa atagagacat atcgttgtag ttattaacag atcactttga ggtcgagggc
    75541 ggctaaccct cgctaatatt atcccgctat taccttgcgg gtgcaaataa tcttttattg
    75601 ctttgatgat ttaataaata gcactttttg ctaacaaaaa ctgctgtcaa tatatggcca
    75661 aaaatgtcaa cagaaaaaat aaggtttata atattaagta tcacatgggt aatgggttca
    75721 gggaatcgga atccaccgcc tcacggaaat tcaaaatttg cttcttcttt cctccgtggg
    75781 cggcttcttt ttcgtatcga ggtatttatg aaagcaataa cattagaaca gttcgcagca
    75841 atggcgaatg tatcgaccag aacgctgagg cgctggctaa aaaaaccatc aaccggagtc
    75901 cctttgccat ttaccccacg cggtactcgc caattattct gggagcacga atgcaaagaa
    75961 tggttgtaca gaacagaacg ataataatca cgcatcacat tttgaggtaa acatcatgac
    76021 caatatcgaa agattagaac gctgtgccga tattatgcgg cgcagatgga tttatgaccc
    76081 tgaagcgggt ttattgttta gccgtgaaac aaaagacgtt atcaaaggca gcatgaccaa
    76141 taaagggcat ttggttgtaa cggtacatga aggtaaattc aaagtgacac tcacctatca
    76201 gaaagcctgc tatgtataca catacggcgc ttatgacgag acattatatg aagtgcacca
    76261 cgttaatttg aacaaacagg ataaccgcat caagaatatt cgcctgatgc cgaaagaaaa
    76321 gcaccgccgg atacataaaa acgtccgcaa acttattcgg ctgggccaca cacaatggct
    76381 tcaaccgcaa ttaattgaac aatcactata aacacaggag taaataccat gcattcatta
    76441 gacagttatt ttcagcgcac gacagcccct aaatcagccg cccaggaacg ccgtgaggaa
    76501 ttccatgaga aagtgatgcg ttctgctgat tatattgcgg acaagttcgt ggaaacggtt
    76561 cgtccacttg ttgatgaagt agctgataaa cttcagtctg aaatgccgga agatatggaa
    76621 ggcactgcaa agcggcgact catttgcgaa ttaagtcgtc gttttggcgt gtcaataagc
    76681 gcttttaaat aatcgaaaaa aggggggaaa gataatggac gcatttgagg tactggcaaa
    76741 taaaggcgct gaactggtag cacagcgtga taaaaccgcc aacgaagggg aacgcgcact
    76801 gctaaataaa cagattaaag cgatccgtat ggcccagttt aaattaatca gtaatgaagt
    76861 tatcgaaaca attaaaccgc agatcccaca aatcgttgct gacgctatta aagccgccgg
    76921 actggagaaa cgcattgccg ggctaaaaac gaacgcagag aaaatggaga tgcacaaaca
    76981 gagcggtgct ccgaatcccg atgagtactt ctcaccggat gttgagttta tggcgcaggt
    77041 tgaggaacgt ctgggtagct gtctcaccga ggaacagcgt cgctattttg atggagtgga
    77101 tagttcggca ggtattgacc tgaactcgta tttcggtcgt gaaattgagc attttgatgc
    77161 cgccccgtct atgccggagc cagaaccaga aatggccacc gatcctgaag ctgagcaacg
    77221 gaaacgcgta ttcaatgcga tagggattcg gtactgatcg gataaaagaa agccgggaat
    77281 taccccggct gacttaacaa tgtgaccaat gaacaaatcg gacaagacaa acaggataag
    77341 cagtctcgaa acaatgttcg ggaactatat tagcacaagt ccggagaaaa tcatcatgtt
    77401 ggcactacgc atcatgcagg gcatcgctaa gaccctcgca gaacatgtct tagacctgaa
    77461 acattcgcca ctgagcaaac aggccatgaa gcgccaaacg cttcgtctgt gggctgaata
    77521 ttcgcttgga actatcaata aaatcatcga catgaaatcg gggccgagca accagagcgc
    77581 ggaggaaatg gagttcatca ggcgtttaat tctcattcgc agagacattc atagccagtt
    77641 acacagcgtg ggaatcgata ttaatgatgg gacgggcgat taattttcgg acaagtacta
    77701 tgcgcgcgcg aggagctgaa catgaagcaa gatattgcag acaggctgga gatactcgaa
    77761 ggccagcggg cagaagcgaa acaactacgt aagcaggcca gaagagcgca caggaacaac
    77821 gaagcagagt tactaaccaa atacatcagt ttcaccaatt actgcatcta cgagtgttac
    77881 aaggaagatg cagaggactg gctggattca ttgccagaac agtactgagc agaataacac
    77941 cacgtcatgt tgttctgagg tgagcatatc acctctgttc aggtggccaa atttagtgtg
    78001 ccacttcagc atcaccgttg aaatcacaat aacgattttt ttgtaaatat gctctcacat
    78061 atgtaggaat agaccaggtt ggcattgaaa cacatctacc atgaccataa taaagtaatt
    78121 ttttgatatg tttatggttt tatattaatt aaaatatatc taaaattgat agttttaaaa
    78181 ttgcaaaatc cagattgtgt ttgtaatgtt taatctgatt gtattgagtg gcgtttatta
    78241 tgaaaaataa atcaggtaat tcttgttata gagaagcagt cgatattcat agctcacttg
    78301 agctttcatt tatattgcat aaatatcatt tgcgtgtaga tgagaagaaa tctcgaaatg
    78361 tattcagcgc catacgtgat gagctcatcc ttgatgggga ttcacgacaa aacttagcta
    78421 ctttctgtca aacatggctt gatgatgaaa tccgtgagtt gcttaattta gctattgata
    78481 aaaacatgat ttataaagat gaatatccac aaatggcgga acctgaaggt cgctgtgtgc
    78541 gtatgctttc cgattcgtgg agtttccccg attcgcgaca caccttaggt tgctcaacca
    78601 ttggttcatc tgaagctgct atgctcggcc gcttggcgtt aaaatggcag tggtgtaaaa
    78661 aacgcgaagt acaaggtaaa tcaaccgaac ctgatttgcg atcctgtgca aatatgctgg
    78721 cataaattct gccgttactg tgatgtgaaa ttgtgtgaaa ttccacttga tgatcatcac
    78781 ctgataatga atgctaaaga ggttttgaag catgtcgatg aaaacactat tggtgtcgtt
    78841 acgactttag gtattactta tttttgtaaa gcagattgcg ttagtgtttt taagataagt
    78901 gttaaagtta atattagttt ttatatttac atcgtaatta tatgtttcta ttttaaaaat
    78961 gttcgagagt ataaaaatgt ctaatgctaa tatcgcaccc aaaagtaaga aaatgacagt
    79021 ggggacactc gctatcatga atataactgc tgtagtaagt ctacgattta taccttctga
    79081 agcagaatac ggcttaggtg ccatttttta ctataccttt gctgcaatta tgtttttagt
    79141 acctgttgcc tttgttgcgg ctgagctagc tacaacatat ccacaaaaag ggggggcatt
    79201 tcgatggata ggagaagcat ttggggctcg ctggggtttt gtaggaatgc ttatgacctg
    79261 gctacaagtc atcccctact ttccaacagt actgacattt ggtgcagtct cggttgcatt
    79321 tattgatgtt gatatggggg tggcagaatc aattgccagt aataaatggt atattttatt
    79381 tttcgtacta tttgtttact ggggagctat ttgtgttgca ttacgtggcg ttggtatttt
    79441 ctcgcaggta tctaagtggt gcggtattat agggacaatt attccagcta ttatccttgt
    79501 tattcttggt ttctcctatc tgtggttcag cggtaaacca gcggtgatcc cattaggttg
    79561 gggtgatctt ttccctaata tagctaactt ccagaatatt gttcttgccg ccagcatatt
    79621 tcttgcttat gcagggatgg aaatgaatgc ggtccatgtt aacgatttag ataatccaac
    79681 taaaaaatac ccaattgcaa ttaccatcgc ttctataggt actattgcaa tattcctgct
    79741 cagtacttta ggggtcgcct ttattattcc tacagataaa attagtctaa ctcaaagttt
    79801 gttggttgcc tacgatatgc tgttcgaatg ggctggagtg ccatggcttg gctctgtgat
    79861 ggcatttatg ctagcaattg gcgtactggg tggggtggtt acttggattg cagggcctaa
    79921 tacaggtgta ttagcgatcg cgaaagctgg ttatttacct aagtttttcc aaaaaaccaa
    79981 tcgtcatgga atggggcacc atctaatgtt tgtacaaggt ataattgtct cagttctttc
    80041 tgttactttt gtgataatgc cttcagttca agcagctttt cagattttat cacagttaac
    80101 agttatgctc tatttagtta tgtatatgct catgttcgct agtgctatct atctacgaca
    80161 ttctcaacct gatacaaaac gcccatatcg agcccctgct ttatttttct gggcaacact
    80221 tggattctta gggtcttttc tggcttttgt attaagtttt attccgcccg agcaaatccc
    80281 aactggtagt ccaaccagct atgttcttta tttgttagta ttagttgccc tatttttatt
    80341 aattccaata ttaatttacg ccttccgaaa atctgactgg aaagatccaa actctgattt
    80401 cgaaccattt tcttgggaaa aaagaactag taattaaatt ttccataagc tacaaaagat
    80461 gaggttaaat atgtttagtg ctgaatctcc aattggtgta atagactcag gtgtaggtgg
    80521 attgacatcg gtaaaggaaa taattaattt gcttcctggt gaagatatta tctattgtgg
    80581 agataatatg aatgcacctt atggcaaccg ttctgccgat gatatcatca gtttgactaa
    80641 aaaaatgctg acttttttac agtcaaggaa tgtcaaacta gttgctgtcg catgtaatac
    80701 tatatcctca actttggaat ccgaagaata cagcggatat gctaagagct ttccgtttcc
    80761 tatcttatcc atcattgaac ctgcagtaga agatgtgatt cgccagcaat ataaaaatgt
    80821 tggtattatc gctactgagt ttactattaa aacggggtgt cacaaagagc taataaaaaa
    80881 attgaattca acaataaatg tatttggaga accaagcaaa aatcttgcaa tgttaattga
    80941 ggaaggtaat cttaatgctc ctgcaatctt gaatgacatc aaaaaacatg ttaaccattt
    81001 aatttccctc catccagtaa atgaaattat tctcggctgt actcactatc caattgttca
    81061 gaattttttt gaagcagttg caccggatat aaaatttatt aatcctgcac atgatcaggc
    81121 tatctctatt aaaaatcact tgggacagct aaatttatta aactcctcaa atataggaac
    81181 tttgcaaata aatactagtg gaagtatgga gatctacaaa acggtactag gggaattgag
    81241 cattacgaaa cctcatacat tcagtatcag gcagttttaa atttatccgg tgcaccgtaa
    81301 aatcccgtcc ttctgaactg caccccaaaa gttggacacc aactgagtaa ggtgcatttt
    81361 atggacaaac ccagttacat actgggtagt gcatccaatt agtagaacat gtgtttttcg
    81421 ataaacgctc cgatcacttt ttcgtggatc tcaactgagc gtgagaagca gattgtttta
    81481 cgacccaacc gcttaatgcg ggtgcgtagc gtcagattat tacgctcaat gcgttgggtg
    81541 aatattttgc cggtcagatg cttattcttc ggcacctccc ggccatagct gccccagtca
    81601 tcgctggtca gcatgccgat gttgaagggt gtaagcagtg ccagtagctc ccggcacgtt
    81661 tgatcggttc ggggaccaaa agtgtaggcc agtacaccgc ctgttttggt gttgtacgcg
    81721 tacccgagcc aatgttgccg ggctttactg ccaacgtagc tccattgctc atcaagctcg
    81781 cagataaacg ccacatcagc atgggcaaca ggcgaagaag ttattcgctt tggcgcgagt
    81841 tttttaaagt ccggatgacg gtgttactac caattttcag tgtcctggcg gtatcgcgaa
    81901 ccccggcacc attaaaggcc atttcagtta tcagctcttt catacccggc ttacgtgctt
    81961 gataagtata agtgaactga aacacacggt ggcagtcacg gcagcgaagt ctgtcacggc
    82021 ctttagggtt ctgaccatgg cggtaaacct gagctgactg acaacgggga caatgaatat
    82081 taacgctggt catgagataa cctcaaaagc ccgtattata catcagattc aactaattag
    82141 aggcatcacc catccagcat tataaaacat actaacaata actaaattat aataatcaca
    82201 acttacaatc aaataataaa tgcataagat taacaatgat tttttcgggt tatatttatc
    82261 taaaaaagca gaatattatg taacttatta ttttctaagc gaaaatttat tgtgcggtca
    82321 gactaacaaa cttgcgtacc acccgaagaa catgcgaaga tttaagtatt ctttcttttt
    82381 tggtttttat ttttcatttt ctcttttctt ctctttccgg ttctgttctg gttttggttt
    82441 tttccttttt atttttattc tttcttcagg gttatgctaa aacaccaaaa catttttcac
    82501 cacaaaagca tccaatcatc ttgccagacc aatcacctta accggataca ctgccggata
    82561 taatcccact aacacccaga caccaagcac catacctgat aactaccgta ttcaggaagg
    82621 aaatgcgagt gatttcaccg tcaccggcac aaccgatccg ccaaaaagag gcgtaccaat
    82681 gtcgatatag tcccccgcgc ggacaatcac ttcgtcaatg actgccaacg ggagttgttg
    82741 tagctgtggg cgcaacaaca tgcccagagc tttaccaaag tcctgcccgg ccaccaccag
    82801 caggggatgc ggattcggaa aacgcgcgac gaaatcgacc agcgcgttga tgaccgtcag
    82861 aaccgcggcg taacgcacag gcagcgaggc gggaagcgcc agcacgtaca cgtcagtttt
    82921 gggatcaaga tccagctgaa gcagcgcctg ttgccaggca ctcaccagat ccgtttcatc
    82981 aatcgggatc gccaccggca aattgcgcag cggcagttgt acgccctcca gccagattgt
    83041 gctgccagag agcgaaaggg tatgtgcacc cgcgccaatc accgtggagc gtacggtttg
    83101 cgccggaaac tgcacattca tctcacgcag gcgcggatgg tcatgcagcg ccgttgccag
    83161 aagtgggcca atatcggcaa aacagaacgg gttggcgggc tggtggcgat aacattcgcc
    83221 cacgcctcca gaaagcgtaa tgatttcggg cgtaacacct gcgggcagca aaccggtttg
    83281 catcaatgcc tgcgcgagcg gcgaaagcgt tccgtcaatc acttcgacaa tcagcgccgc
    83341 catccgccga gtaacctgca ccagctgcgc gccggtcagc gaacgggcgt cagtgcctgc
    83401 accgaagcac tcatccacaa tcatctgccc cggtttatga gcgtaaacca cgcgcccctg
    83461 gctgtcggtt tccagcaggc gaccaccgac gttgagacag gcagtgccgc tgatttttcc
    83521 ggcatcgaac agggcgtagt tcgcggtgcc accgccgata tcgatattca gtacccgaca
    83581 cagccgttgt tcagaaaggg tttgcgcccc ggctccgtga ccggcgatca cggattcgag
    83641 gtgcggcccg gcgctggcga cgacaaaatc gcccagcgac tgtttttcct tcatcacttt
    83701 ttccatcagc tgtgcgacca ggctttcggt aaactggcct tccggcagct gggcaatgat
    83761 ggtttcgcgg atgcgctggc tttcggtttt gtcggctggc acgctggcgg gcacggacgc
    83821 cgttaccggg atcgattcat cacattcggt gatggtaaaa cccagtagat ccgccacttc
    83881 acgggcttcc ggggtaataa tgctggcgcg cagaaccacg gacattgcct gttcgccgcg
    83941 cgcgtgtgct tcacgaatat cattcgctgt gatgagtttt ttcacctgct tcctcctgtt
    84001 tggtgagaga tgccagataa tccaccagtt gctgcacgct ttgcgggtcg tggctgttta
    84061 gctcgaaaat cggctcttcg aaacccgtct caagcaataa ctttcgcgtt gcggcgacgt
    84121 cagcatctgg catgtccgtt ttactgatga cggcgatttg ccgtttgctg acgccaatat
    84181 ccagcaaccc ggcaggcaac cgactttccg ggtcatttgc gccatggaca tagatcagca
    84241 tgtctacatc ctgtagcgtg gtaattaagg cgtgatacca gcggggatgg ctaaaatatt
    84301 cacccggcgt atcaatatcg ccc
//
LOCUS       DAJTQY010000002        78784 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.002, whole genome shotgun sequence.
ACCESSION   DAJTQY010000002 DAJTQY010000000
VERSION     DAJTQY010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 78784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 78784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..78784
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.002"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            <1..228
                     /locus_tag="OO349_000086"
     CDS             <1..228
                     /locus_tag="OO349_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259191.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
                     /protein_id="HCR5865315.1"
                     /translation="ESNSDSAPYTLLKNYADRFKKYSAEEQYEIDFQHRLVNRNCYLD
                     KYGDARINASYTQLEIKHSQPKNRASGKRVS"
     gene            648..1263
                     /locus_tag="OO349_000087"
                     /pseudo
     CDS             648..1263
                     /locus_tag="OO349_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188400.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1366..1689
                     /locus_tag="OO349_000088"
     CDS             1366..1689
                     /locus_tag="OO349_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1877 family protein"
                     /protein_id="HCR5865316.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLESLD
                     RSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNHAL
                     TVIVG"
     gene            complement(1756..3213)
                     /gene="nuoN"
                     /locus_tag="OO349_000089"
     CDS             complement(1756..3213)
                     /gene="nuoN"
                     /locus_tag="OO349_000089"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HCR5865317.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGMMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(3220..4749)
                     /gene="nuoM"
                     /locus_tag="OO349_000090"
     CDS             complement(3220..4749)
                     /gene="nuoM"
                     /locus_tag="OO349_000090"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HCR5865318.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMTFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4913..6754)
                     /gene="nuoL"
                     /locus_tag="OO349_000091"
     CDS             complement(4913..6754)
                     /gene="nuoL"
                     /locus_tag="OO349_000091"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HCR5865319.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRSLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVFSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6751..7053)
                     /gene="nuoK"
                     /locus_tag="OO349_000092"
     CDS             complement(6751..7053)
                     /gene="nuoK"
                     /locus_tag="OO349_000092"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HCR5865320.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(7050..7604)
                     /gene="nuoJ"
                     /locus_tag="OO349_000093"
     CDS             complement(7050..7604)
                     /gene="nuoJ"
                     /locus_tag="OO349_000093"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HCR5865321.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7616..8158)
                     /gene="nuoI"
                     /locus_tag="OO349_000094"
     CDS             complement(7616..8158)
                     /gene="nuoI"
                     /locus_tag="OO349_000094"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HCR5865322.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(8173..9150)
                     /gene="nuoH"
                     /locus_tag="OO349_000095"
     CDS             complement(8173..9150)
                     /gene="nuoH"
                     /locus_tag="OO349_000095"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HCR5865323.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDRVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(9147..11873)
                     /gene="nuoG"
                     /locus_tag="OO349_000096"
     CDS             complement(9147..11873)
                     /gene="nuoG"
                     /locus_tag="OO349_000096"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HCR5865324.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQNKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRYASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSSASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11926..13263)
                     /gene="nuoF"
                     /locus_tag="OO349_000097"
     CDS             complement(11926..13263)
                     /gene="nuoF"
                     /locus_tag="OO349_000097"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HCR5865325.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(13260..13760)
                     /gene="nuoE"
                     /locus_tag="OO349_000098"
     CDS             complement(13260..13760)
                     /gene="nuoE"
                     /locus_tag="OO349_000098"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HCR5865326.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13763..15565)
                     /gene="nuoC"
                     /locus_tag="OO349_000099"
     CDS             complement(13763..15565)
                     /gene="nuoC"
                     /locus_tag="OO349_000099"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HCR5865327.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15659..16321)
                     /gene="nuoB"
                     /locus_tag="OO349_000100"
     CDS             complement(15659..16321)
                     /gene="nuoB"
                     /locus_tag="OO349_000100"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HCR5865328.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(16337..16780)
                     /gene="nuoA"
                     /locus_tag="OO349_000101"
     CDS             complement(16337..16780)
                     /gene="nuoA"
                     /locus_tag="OO349_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HCR5865329.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(17411..18349)
                     /gene="lrhA"
                     /locus_tag="OO349_000102"
     CDS             complement(17411..18349)
                     /gene="lrhA"
                     /locus_tag="OO349_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HCR5865330.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKVDIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            19269..20486
                     /gene="alaA"
                     /locus_tag="OO349_000103"
     CDS             19269..20486
                     /gene="alaA"
                     /locus_tag="OO349_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HCR5865331.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            20570..21169
                     /gene="yfbR"
                     /locus_tag="OO349_000104"
     CDS             20570..21169
                     /gene="yfbR"
                     /locus_tag="OO349_000104"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HCR5865332.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNAQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(21228..23060)
                     /locus_tag="OO349_000105"
     CDS             complement(21228..23060)
                     /locus_tag="OO349_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HCR5865333.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTSVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTNEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(23147..23797)
                     /gene="hxpA"
                     /locus_tag="OO349_000106"
     CDS             complement(23147..23797)
                     /gene="hxpA"
                     /locus_tag="OO349_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HCR5865334.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(23808..24302)
                     /locus_tag="OO349_000107"
     CDS             complement(23808..24302)
                     /locus_tag="OO349_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HCR5865335.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALYVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(24385..24840)
                     /locus_tag="OO349_000108"
     CDS             complement(24385..24840)
                     /locus_tag="OO349_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HCR5865336.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGLLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            25178..26380
                     /gene="ackA"
                     /locus_tag="OO349_000109"
     CDS             25178..26380
                     /gene="ackA"
                     /locus_tag="OO349_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HCR5865337.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVAFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            26455..28599
                     /gene="pta"
                     /locus_tag="OO349_000110"
     CDS             26455..28599
                     /gene="pta"
                     /locus_tag="OO349_000110"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCR5865338.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            28789..30309
                     /gene="yfcC"
                     /locus_tag="OO349_000111"
     CDS             28789..30309
                     /gene="yfcC"
                     /locus_tag="OO349_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HCR5865339.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGNASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(30342..30884)
                     /gene="yfcD"
                     /locus_tag="OO349_000112"
     CDS             complement(30342..30884)
                     /gene="yfcD"
                     /locus_tag="OO349_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HCR5865340.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSGVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(30942..31493)
                     /gene="yfcE"
                     /locus_tag="OO349_000113"
     CDS             complement(30942..31493)
                     /gene="yfcE"
                     /locus_tag="OO349_000113"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HCR5865341.1"
                     /translation="MKLMFASDIHGSLPATERVVELFVQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMMLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPAGYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(31549..32193)
                     /gene="yfcF"
                     /locus_tag="OO349_000114"
     CDS             complement(31549..32193)
                     /gene="yfcF"
                     /locus_tag="OO349_000114"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HCR5865342.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            32329..32976
                     /gene="yfcG"
                     /locus_tag="OO349_000115"
     CDS             32329..32976
                     /gene="yfcG"
                     /locus_tag="OO349_000115"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HCR5865343.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRLRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            33033..33395
                     /gene="folX"
                     /locus_tag="OO349_000116"
     CDS             33033..33395
                     /gene="folX"
                     /locus_tag="OO349_000116"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HCR5865344.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            33416..34309
                     /locus_tag="OO349_000117"
     CDS             33416..34309
                     /locus_tag="OO349_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HCR5865345.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHKQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPTTAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(34357..35247)
                     /gene="rpnB"
                     /locus_tag="OO349_000118"
     CDS             complement(34357..35247)
                     /gene="rpnB"
                     /locus_tag="OO349_000118"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HCR5865346.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARNFINIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(35444..36217)
                     /gene="hisP"
                     /locus_tag="OO349_000119"
     CDS             complement(35444..36217)
                     /gene="hisP"
                     /locus_tag="OO349_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HCR5865347.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(36225..36941)
                     /locus_tag="OO349_000120"
     CDS             complement(36225..36941)
                     /locus_tag="OO349_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5865348.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(36938..37624)
                     /gene="hisQ"
                     /locus_tag="OO349_000121"
     CDS             complement(36938..37624)
                     /gene="hisQ"
                     /locus_tag="OO349_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HCR5865349.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNMVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(37714..38496)
                     /gene="hisJ"
                     /locus_tag="OO349_000122"
     CDS             complement(37714..38496)
                     /gene="hisJ"
                     /locus_tag="OO349_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HCR5865350.1"
                     /translation="MKKLVLSLSLVLAFSSATAALAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIELAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(38717..39499)
                     /gene="argT"
                     /locus_tag="OO349_000123"
     CDS             complement(38717..39499)
                     /gene="argT"
                     /locus_tag="OO349_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HCR5865351.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYALFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(39765..40334)
                     /gene="ubiX"
                     /locus_tag="OO349_000124"
     CDS             complement(39765..40334)
                     /gene="ubiX"
                     /locus_tag="OO349_000124"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HCR5865352.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTLDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(40429..41946)
                     /gene="purF"
                     /locus_tag="OO349_000125"
     CDS             complement(40429..41946)
                     /gene="purF"
                     /locus_tag="OO349_000125"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HCR5865353.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(41983..42471)
                     /gene="cvpA"
                     /locus_tag="OO349_000126"
     CDS             complement(41983..42471)
                     /gene="cvpA"
                     /locus_tag="OO349_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HCR5865354.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIHGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(42730..43392)
                     /gene="dedD"
                     /locus_tag="OO349_000127"
     CDS             complement(42730..43392)
                     /gene="dedD"
                     /locus_tag="OO349_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HCR5865355.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(43382..44650)
                     /gene="folC"
                     /locus_tag="OO349_000128"
     CDS             complement(43382..44650)
                     /gene="folC"
                     /locus_tag="OO349_000128"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HCR5865356.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(44720..45634)
                     /gene="accD"
                     /locus_tag="OO349_000129"
     CDS             complement(44720..45634)
                     /gene="accD"
                     /locus_tag="OO349_000129"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HCR5865357.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDELKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQAQEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPRFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(45790..46449)
                     /locus_tag="OO349_000130"
     CDS             complement(45790..46449)
                     /locus_tag="OO349_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5865358.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VMPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            complement(46532..47344)
                     /gene="truA"
                     /locus_tag="OO349_000131"
     CDS             complement(46532..47344)
                     /gene="truA"
                     /locus_tag="OO349_000131"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCR5865359.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(47344..48357)
                     /locus_tag="OO349_000132"
     CDS             complement(47344..48357)
                     /locus_tag="OO349_000132"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCR5865360.1"
                     /translation="MSEGWNIAVLGATGAMGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(48423..49559)
                     /gene="pdxB"
                     /locus_tag="OO349_000133"
     CDS             complement(48423..49559)
                     /gene="pdxB"
                     /locus_tag="OO349_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HCR5865361.1"
                     /translation="MKILVDENMPYARDLFSRLGEVIAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQRADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            49658..50653
                     /gene="flk"
                     /locus_tag="OO349_000134"
     CDS             49658..50653
                     /gene="flk"
                     /locus_tag="OO349_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HCR5865362.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNHRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(50650..51828)
                     /locus_tag="OO349_000135"
     CDS             complement(50650..51828)
                     /locus_tag="OO349_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5865363.1"
                     /translation="MTAVSQTETRSSASFSLFRISFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(52083..53324)
                     /gene="fabB"
                     /locus_tag="OO349_000136"
     CDS             complement(52083..53324)
                     /gene="fabB"
                     /locus_tag="OO349_000136"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HCR5865364.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNIKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAYIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKRLIRSRPE"
     gene            53483..55489
                     /gene="mnmC"
                     /locus_tag="OO349_000137"
     CDS             53483..55489
                     /gene="mnmC"
                     /locus_tag="OO349_000137"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HCR5865365.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVIAESGFGTGLNFLTLWKAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(55610..55888)
                     /locus_tag="OO349_000138"
     CDS             complement(55610..55888)
                     /locus_tag="OO349_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HCR5865366.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQL"
     gene            complement(55922..56470)
                     /gene="epmC"
                     /locus_tag="OO349_000139"
     CDS             complement(55922..56470)
                     /gene="epmC"
                     /locus_tag="OO349_000139"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HCR5865367.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     AFDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(56470..57279)
                     /locus_tag="OO349_000140"
     CDS             complement(56470..57279)
                     /locus_tag="OO349_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HCR5865368.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVMLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(57279..58103)
                     /gene="mepA"
                     /locus_tag="OO349_000141"
     CDS             complement(57279..58103)
                     /gene="mepA"
                     /locus_tag="OO349_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HCR5865369.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(58107..59192)
                     /gene="aroC"
                     /locus_tag="OO349_000142"
     CDS             complement(58107..59192)
                     /gene="aroC"
                     /locus_tag="OO349_000142"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HCR5865370.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     FGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(59227..60159)
                     /gene="prmB"
                     /locus_tag="OO349_000143"
     CDS             complement(59227..60159)
                     /gene="prmB"
                     /locus_tag="OO349_000143"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCR5865371.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLIAAREHFAIYKD"
     gene            60325..60876
                     /gene="smrB"
                     /locus_tag="OO349_000144"
     CDS             60325..60876
                     /gene="smrB"
                     /locus_tag="OO349_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HCR5865372.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(60980..61870)
                     /locus_tag="OO349_000145"
     CDS             complement(60980..61870)
                     /locus_tag="OO349_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HCR5865373.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVAYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVASYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNELKFVVLL"
     gene            complement(61857..62381)
                     /locus_tag="OO349_000146"
     CDS             complement(61857..62381)
                     /locus_tag="OO349_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5865374.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            complement(62378..62848)
                     /locus_tag="OO349_000147"
     CDS             complement(62378..62848)
                     /locus_tag="OO349_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5865375.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTPSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLVVNETDLPVLTAVPVKKSGVILPEADFEAWATLQVDYQ"
     gene            complement(62845..63393)
                     /locus_tag="OO349_000148"
     CDS             complement(62845..63393)
                     /locus_tag="OO349_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5865376.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINRVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            complement(63398..64120)
                     /locus_tag="OO349_000149"
                     /pseudo
     CDS             complement(63398..64120)
                     /locus_tag="OO349_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            complement(64140..64862)
                     /locus_tag="OO349_000150"
     CDS             complement(64140..64862)
                     /locus_tag="OO349_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001433219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCR5865377.1"
                     /translation="MAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNMGGT
                     RLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNKLPENAEATQS
                     VVQATLTEGAIGYRKFAVISGQKAMAVLRLQDGSHPPFGAEVKNDNEQTVGLVDDDGN
                     VYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPVVPDDIKP
                     VIQEQTQQVTPTNPPVSVSANQ"
     gene            complement(64922..66154)
                     /locus_tag="OO349_000151"
     CDS             complement(64922..66154)
                     /locus_tag="OO349_000151"
                     /inference="COORDINATES: protein motif:HMM:NF012786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCR5865378.1"
                     /translation="MRADWQTNYQHTRSNDDDEFSGDETQKKWEWSRYYARRALPSLK
                     AKLALGEDYLRSDIFDGFNYVGGSVSTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQM
                     GRVIYETQVPAGPFRIQDLGDSVSGTLHIRIEEQNGQVQEYDISTASMPYLTRPGQVR
                     YKIMMGRPQEWGHHVEGEFFSGAEASWGIANGWSLYGGALGDENYQSAALGVGRDLST
                     FGAVAFDVTHSHTKLDKDTAYGKGSLDGNSFRVSYSKDFDQLNSRVTFAGYRFSEENF
                     MTMSEYLDASDSGMVRTGNDKEMYTATYNQNFRDAGVSVYLNYTRHTYWDREEQTNYN
                     IMLSHYFNMGSIRNVSISMTGYRYEYDNQADKGMYISLSMPWGDNSTVSYNGTMAVGR
                     TAVRSVISAVSMTRLTIS"
     gene            complement(66203..66781)
                     /locus_tag="OO349_000152"
                     /pseudo
     CDS             complement(66203..66781)
                     /locus_tag="OO349_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001499769.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(66863..67426)
                     /locus_tag="OO349_000153"
     CDS             complement(66863..67426)
                     /locus_tag="OO349_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5865379.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     NAWLVGAADAPDLGNFEANTTFQITYL"
     gene            complement(68110..68595)
                     /gene="sixA"
                     /locus_tag="OO349_000154"
     CDS             complement(68110..68595)
                     /gene="sixA"
                     /locus_tag="OO349_000154"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HCR5865380.1"
                     /translation="MQVFIMRHGDAALDSASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(68798..70942)
                     /gene="fadJ"
                     /locus_tag="OO349_000155"
                     /pseudo
     CDS             complement(68798..70942)
                     /gene="fadJ"
                     /locus_tag="OO349_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
     gene            complement(70942..72252)
                     /gene="fadI"
                     /locus_tag="OO349_000156"
     CDS             complement(70942..72252)
                     /gene="fadI"
                     /locus_tag="OO349_000156"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HCR5865381.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPTVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLAHVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(72432..72716)
                     /locus_tag="OO349_000157"
     CDS             complement(72432..72716)
                     /locus_tag="OO349_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HCR5865382.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKSRSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            73088..74428
                     /gene="fadL"
                     /locus_tag="OO349_000158"
     CDS             73088..74428
                     /gene="fadL"
                     /locus_tag="OO349_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HCR5865383.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAIYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            75179..75853
                     /locus_tag="OO349_000159"
     CDS             75179..75853
                     /locus_tag="OO349_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5865384.1"
                     /translation="MIFSALKEIYDGDPGFIFDKISHKLRHTVTEFDESGKSEPTDLF
                     TWYGKDKKGDSLAIVIKNKNGNDYLFLGYYDQDDYHIQRGIRINGDSLTQYCSENARS
                     ASAWFESSKAIMAESFATGSDHQVVNELNGERLREPNEVFKRLGRAIRYNFQVDDAKF
                     RRDNVKEIISTLFANKVDVDHPENKYKDFKNLEDKVEKRLQNRQTKYQNEINQLSALG
                     VNFDDI"
     gene            complement(76035..76790)
                     /gene="mlaA"
                     /locus_tag="OO349_000160"
     CDS             complement(76035..76790)
                     /gene="mlaA"
                     /locus_tag="OO349_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HCR5865385.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADALYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            77084..78016
                     /locus_tag="OO349_000161"
     CDS             77084..78016
                     /locus_tag="OO349_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HCR5865386.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHAELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            78090..78164
                     /locus_tag="OO349_000162"
     tRNA            78090..78164
                     /locus_tag="OO349_000162"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:78123..78125,aa:Arg,seq:cct)
     gene            78326..>78784
                     /gene="intS"
                     /locus_tag="OO349_000163"
     CDS             78326..>78784
                     /gene="intS"
                     /locus_tag="OO349_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HCR5865387.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISAGIDPVKAKKASSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGA"
BASE COUNT        19967 a        21038 c        19327 g        18452 t
ORIGIN      
        1 gaaagcaatt ctgacagcgc cccctatacg ttattaaaaa actatgctga cagatttaaa
       61 aaatattcag cagaagagca gtacgaaatt gattttcaac atcgactggt aaacagaaat
      121 tgttatttag ataaatatgg cgatgcacga atcaatgctt cttatacaca attagaaata
      181 aaacactcac aacccaaaaa tagagcatcc ggcaaaagag tgagttaaca tcattgttaa
      241 tcaattcgac cagtcagtaa atatgctgac tggtcgaaca taatttgcta aaaagtcatc
      301 actgactgat gtcagtccca ccatctgcca gttcaatcta cgggtatttc cagcatatcg
      361 cggcacgcga ttgctggtct gttgatgtag tggattgtag ttgctgactt ttcttaccct
      421 cagatttctg ccccacttgt caattcaatc tgccaataaa taacataatt atatttttat
      481 tcctcacaga aattcctttt taaaaatagc gaacccactg taaaaaacac tttttgtata
      541 cctctaccaa gcgtaataaa ataaacgctc ataattcatt ataaataata atgaaataac
      601 gccttttatt gataatttac atatgaaaaa aattttcctt ttgttatatt atttctgttt
      661 tcactccctc tgattatttt taccaaccct gggtcactgc attatcgcca acgccccgac
      721 acgtaagtcc tgagcaatta gaaaaaacag ttcgttatct tacacaaact gtgcatccac
      781 gtaatgccgg caatattgat aatctgaata ggtccgcaga gtatataaaa gagttctttg
      841 tcagtagcgg tgccagagtt accgcgcagg gcgtccccga ttggctatta cgactctgcg
      901 cctggaagcc agaattaccc ttacccggca atgtcctggc tttatcccga tcggggagac
      961 tttattgctg tggtcggcag aatgcaggat atcaacgccg ttcgtcaggt aaaagcggca
     1021 ttgttgtcat cccgggattt atctgtttat tctataaatg ccccagggtt tattcccggt
     1081 attgattttt ctgatcacct caattattgg caacacgata ttcccgccgt aatgattact
     1141 gacaccgctt tttatcgtaa taaacaatac cacttgcccg gtgataccgc agacagattg
     1201 aattatcaga aaatggctca ggtggtagat ggtgttataa ccttgttata caacagtaaa
     1261 taataaaaat ttctcgaggc gggtccagaa acagcgacat gtacatttta ttttccctcg
     1321 tctgttttaa gatgcgaaac ataaaattca atggaggaaa aataaatggg aatgattggc
     1381 tattttgcag aaattgactc agaaaagatt aaccaactgc ttgagtctac tgaaaaacct
     1441 ttgatggata atatacatga cacgctttca ggattacgcc gactggaatc actggatcga
     1501 agtgagctgc gtaaacagtt ttccattaag cgactcaatg aaatggagat ttatccggga
     1561 gtgacctttt cagaagagtt agaggggcaa ctctttgcct cgatcatgct tgatatggaa
     1621 aagctgatta gcgcgtatcg acgtatgctg cgacaaggga atcatgcgct gacggttatt
     1681 gttgggtgat ggtgtcagag cgccaaaaaa aaccgccgaa catgtcggcg gctttctgac
     1741 ttacaaacta acagtttaca tcagcggcat tgctaaccgc acaatactaa tcagcggttg
     1801 tggccataca cccagcacca gtaccaacag tgcagaaatc agcaccacga taccgcccgc
     1861 gctgtactgc cagtttgacg gtgcatcgcg acccggttgt tccggtgcgt gcaggtacag
     1921 gctcaccgcc acgcgcaggt agtagtagag gccgattgcc gaaccgacaa ccacggcacc
     1981 caccagccac cacaagtgtg cctggacacc gaccgccagc acgtagaact taccgataaa
     2041 gcccagcgtc atcgggatac cggccagaga cagcatcatc accgtcatca ctgccgcgag
     2101 gatcggacga tgccagaaca gaccgcggta agagaacagg gaatcagcat ccgggccacg
     2161 atacgggctg gacatcaggc tgaccacgcc gaacgcgccg aggctgctga acagataacc
     2221 ggccaggtaa acccctaccg cttccatcga catctcgccg gtttgcagcg caatcagcgc
     2281 caccagcaga tagccgaggt gagagataga tgagtaaccg agcagacgtt tgatgttggt
     2341 ctggctcaac gccatcaggt taccgaagat gatggaggca aaggcgataa tcgccagtac
     2401 tacgcgaatc gcttcgctgt cacccaccgg tgcgtagagg aacagacgca tcaccacacc
     2461 gaagatagcg attttgctcg ccgtcgccag gaaagtggaa accggcgcag gcgcgccctg
     2521 gtatacgtct ggcgtccaca ggtggaacgg caccagagag agtttgaagc cgaggccaac
     2581 aatcatcatg ccgaaacctg ccagcaacag cggctcgttg agcataccgt cgccgaggtt
     2641 tttacccagc gcgacaaacg acaggtcgcc agactgcgca tacaccagcg ccataccgaa
     2701 cagcaggaaa gaagacgctg cggcagaaag gatggtgtat ttgatactgg cttccagcga
     2761 acgtttctgg cggaaagcgt aaccgaccag gccaaacagc ggcaaagaga tcagttcaat
     2821 accgaggaac agagacgcca gatggtttgc attcgccagc aggatcccgc ccagcgcggc
     2881 aattaacacc aacaggtaga actcatcctt gttgtcgtta tagccttcaa gccacgggta
     2941 ggcgaaagta caggtggcga ggctcgccaa caataccagc ccggtgtaaa gcatggcgaa
     3001 accatcaacg cgcatcagcg gcgtaacgtc catagcgccc gcctggccaa caaaccagag
     3061 cgaaaccagc gccgcgttaa gcccaataac cgagagcgta gcgttgagga aatgattgcg
     3121 tcgccacgca atggagagca tcacaaccac caccgtcaag ccgacgatca gcaacggtag
     3181 cagtgcgatc aggttttgtg gagttattgt catggcgatt tacggccttg tagtagtaac
     3241 ggaattaaca aaccactgct ggatattgcc aatcgcggag tgcgaggtat ccagaatcgg
     3301 ctgcggatag aagcccagca gtaccagcag caccaccagc aacaggatca taaacagctc
     3361 acgcagcgac atccctggca gttcctggct ggcaatctgg cttttcgctt taccgaagta
     3421 agcgcgatgc aacatcgcca gcgaataaac agatgcaaag accagcccaa aggtagagat
     3481 aacggtaatc accgggacaa cctggaagct gccgaacaga atcataaatt cgccgacgaa
     3541 gttaccggtg ccaggcatcc caagcgttgc caccgcaaag aacagcgaca gtgctggcag
     3601 ccatttcatc ttgctccaca gaccgcccat catgcgcatg tcgcgggtat ggatacgttc
     3661 ataaagctga ccgcagagaa taaacagacc cgccgccgac aagccgtgcg caatcatctg
     3721 gattaccgcg ccctggtagg ccaactggct gccggtgtag attgcaatca gcacgaagcc
     3781 catgtgggaa accgaggtgt aggcgatcag acgtttgata tcggtctgag cgaaggtcat
     3841 ccacgcaccg tagaagatgc cgataacacc cagccacata gcgattggcg cgaactctgc
     3901 cgacgcgttc gggaacagcg gcagggagaa acgcaacaaa ccgtaagcgg cggttttcag
     3961 caagatcccc gcgaggtcca cggaaccggc ggtcggagcc tgggagtgcg catccggcag
     4021 ccagccatgc agcggaacca ccggcatttt gactgcgaag gcgatgaaga agcccagcat
     4081 caacagatat tccacaccac tggacattgg cgtattcagc agctcttcat agttgaaggt
     4141 ccagacgccg gtcgcattgt agtgaacaaa aaccagcgcc aggatggcga tcaacatcac
     4201 cagaccactc gcctgggtgt aaatgaagaa cttggttgcc gccgtgatac gcgttttacc
     4261 gtcagaggct ttatgccccc acagtgcgat caggaagtac atcggcacca gcatcatttc
     4321 ccagaagaag aagaacagga acatgtcgat ggcaaggaac acgccgataa cgccgcccag
     4381 gatccacatc aggttgaggt ggaagaagcc ctgatatttt tcgatctctt tccacgaaca
     4441 tagtaccgcc agcacaccga gcagaccggt cagcacgacc atcagcagcg acagaccgtc
     4501 aatggcgaga tgaatagaga taccaaaacg cgggatccac ggcatgtcga attcagactg
     4561 ccactgcgga attccggcgg attgcgtcag tgaataaccg ccctgcaacc acagttgcag
     4621 cgacagcgcc agcgtcaatc ccatggtgat cagcgcgatc cagcgtggca ccttgacgcc
     4681 aaagcgttcg gtctgccagc acaggaagcc gccaataaag ggaattaata ttagccaggg
     4741 tagtaacatg gcgatcttta ttccttgtaa aagtcccgtc aggaccggat tttcaacgaa
     4801 ttctcacgac agaaaattct cttcggactg ggggtagtgt cggatgcgcc gcgaaggcag
     4861 cttatccgac ctacgttctt atatgcccag gggcaatccc acaatcctta actcaacgca
     4921 gtaccatcaa cagtgccagc accacgaccg caccgatgct catggatgcc acataccagc
     4981 gcagataacc gttctcgctt aacagcagac ctttacctgc aaagcgggaa aaaaccgccg
     5041 ggatgttcat cattgagttc agcggatcgc gtttcagcaa ccaggcaata cccaggaacg
     5101 gcttgacgaa cactttgtcg tacagccagt caaagcccca agcgttgtac caccaggtac
     5161 tcagcaggcg gcccggcgca ctgttggcga tggaggtcac cagagaacgt ttacccagcc
     5221 acagccaggc tgccagcaga atgccgacca ccgcgaccac gccagaggta atttccaggg
     5281 tcaacatgct gccgtgcgcc agttccgtcg tttgcggaag cacgccctgc agcggcggta
     5341 caatcagtgc gccaacgaag gtggaaagga tcagcagcac aatcagcggc aggctgtgag
     5401 ttaccccttt cacggcgtga gcgtgaattt gttcttttcc gtggaagacg atgaaaatca
     5461 tacggaaggt gtagagcgag gtcataaacg caccgaccag acctgccacc atcagattga
     5521 tatgaccatt cgccatcgca cccgcgagga tctcatcctt actgaagaag cccgcagtga
     5581 ccagcggcag tgccgacagt gctgcgccgc ccaccaggaa gcagagataa accagcggaa
     5641 tagatttacg cagaccgccc atcttgaaga tgttctgttc gtgatggcag gccagaatga
     5701 cggaaccgga tgccaggaac agcagcgctt taaagaacgc gtgagtcatc aagtggaaaa
     5761 tcgccgcatc ccatgcctgc acgccaagcg cgaggaacat gtagccaatc tggctcatgg
     5821 tagagtaagc gagaacacgt ttgatgtcgg tctgtaccag cgcggcaaaa ccggccagca
     5881 gcagcgtaac cgccccgaca atacccacca gatgcagaac ttccggcgtc atcaggaaca
     5941 ggccgtgggt acgggcgatc aggtagacac ccgcggttac catcgttgcg gcgtggatca
     6001 gcgcggagac aggcgtcggg cccgccatcg cgtcagcaag ccatgtctgc aacggcaact
     6061 gcgcagattt accgaccgca ccgcccagca gcatcagcgt cgcccacatc agcatgttat
     6121 tgccgtcagc aaagtgcgct ggtgccagtt ccaccatttc gcggaagttc agggtgccca
     6181 gttcgttgta aagaatgaac agtgcgaaag cgaggaaaac gtcacccaca cgggtcacga
     6241 cgaacgcttt cattgctgct gcgccattct tcggatcggt gtaatagaaa ccgatcagca
     6301 gataggagca caggcccacg ccttcccagc cgaggtacat cagcagcagg ttatcggcga
     6361 gaaccagaac taccatgctg gcgatgaaca ggttggtgta ggcgaagaag cgagagtagc
     6421 cctcttcacc gcgcatgtac caggaggcgt acatgtgaat aaggaaaccc acaccagtga
     6481 ccaccgagag catggtcagc gacaggccgt ccagcaccag gttaaaaccg atgttaaagt
     6541 cgcctaccga catccacgtc cacagcggct ggctgtatgc ctgctcgccg ttagcgaaga
     6601 aatcaacgcc gataaaggcg gttaccagcg ccgccaggcc cacagagcca acgccgacga
     6661 tcgccgaaac gttttcagac cagcgcccac gggagaacgc cagcaggacg aagccaatca
     6721 atggcaaaat aatggttaag gcaagcatgt tcatccgcgc atctcactta ctgaatcgat
     6781 gttcaggttc tggcgacgac ggtgaagttg cagcagcagc gcaaggccga tactcgcttc
     6841 tgccgccgcg aggctgatgg cgagaatgta catcacctga ccgtcggtct gaccccagta
     6901 gcttccggcg accacaaagg ccagcgcgga ggcgttaatc atgatttcca gaccaatcag
     6961 cataaacagc agattgcgac ggataaccag accggttaag ccaagaacga ataagattgc
     7021 cgcgaggatc agtccatgtt gtaaggggat catgcgtgct cctccgtttt tcttttcgcg
     7081 ctgtcgtctt tacgattgct cagcacttca cccgcacgct cttcacgacc gacgtggaag
     7141 gccacaacca gacccgcgag cagcagcata gaagccagtt ccaccgccag tacgtaaggc
     7201 ccgaacagcg taatacccac tgctttagca ctgattggcg taccgtcgat accctgatcg
     7261 ttaacaccga ggatggcgta aacaatcacc accagcatga tggccgacaa aattgccggg
     7321 ccaatccaca cctgcggttt cagccactgg cgttcctgtt cgatttctga accgcccagg
     7381 ttgagcatca tcaccacgaa cacgaacagc accataatgg cacccgcgta gacgataatt
     7441 tccagcgcac cggcgaagta agcgcctagt gagaagaaca ccccggaaat cgccagcagc
     7501 gaaataatca ggtacagcag tgcgtgtacc ggattggtat gggtgatcac tcgcaaggtc
     7561 gcaagtatgg ctatcaggcc acagatataa aaagcgaact ccattgcccc tctccttacg
     7621 gtaacaggct cttgacgtcg ataggcttgg cttcgttctc tgcttcgccc ttatctttgc
     7681 cgtcgattgc catacctgcc atccggtaga agttatattc cgggtatttg cccggaccgg
     7741 agatcagcag atcctctttc tcgtaaacca gatcctggcg cttgtattcc cccatttcga
     7801 aatccggggt taactgaatc gccgtggtcg gacaggcttc ttcgcacaga ccacagaaaa
     7861 tgcagcgtga gaagttaatg cggaaaaatt ccggatacca gcgaccgtct ttggtttctg
     7921 ctttttgcag cgagatacag ccgaccgggc aggctaccgc acagaggtta caggctacgc
     7981 aacgctcttc gccgtccggg tcgcgggtca gaacgatacg accacgataa cggggcggca
     8041 gatagaccgg ctcttccggg tacattcgcg tttcgcgttt ggcgaacgcg tgcaggccga
     8101 tcatccagat actacgaacc tgggtgccga aacctactaa caattctttt aaggtcatgg
     8161 tcttattgcc ccttattgcg cctgccagag aatgacagcc gccgttacca gcaagttgat
     8221 cagcgtcagc ggcaggcaga ttttccagcc gaaggacatt acccggtcat aacgcggacg
     8281 cggtaacgac gcacgaatca aaatgaacat catcataaag aacgcggttt tcagcgcgaa
     8341 ccagatgaat ggcggtaaca acgggccttg ccagccaccg aagaacagcg tcaccatcaa
     8401 tgcagagatg gtcacaatcc cgatgtactc acccacgaag aacagaccga acttcatacc
     8461 ggaatattca atgtggtaac catccgccag ttcctgctcg gcttccggct ggtcaaacgg
     8521 gtgacggtga cacaccgcca cgcccgcgat ggcaaaggta ataaaaccaa agaattgcgg
     8581 gatgacgttc cacacatgcg cctggctgtt gacgatgtcg gtcatgttga atgaaccggc
     8641 ctgcgccacc acgcccatca gggaaagccc gaggaacact tcgtagctca gggtctgcgc
     8701 agaagcacgc atcgcaccca gcagcgagta tttgttgtta cttgaccagc ccgcaaacag
     8761 caccgcgtaa accgccagac ctgccatcat caggaagaac aaaatcccga tgttcaggtc
     8821 ggcaaccacc caacccggac tgactggcac aattgcaaag gccagcagca gcgaggtaaa
     8881 ggcaatcatc ggtgccaggg taaagatgac gcgatccgag aatttcggga tccagtcttc
     8941 tttaaagaac attttgatca tgtccgcaac cagctggagc gaaccgcccc agccaacacg
     9001 gttaggtcca taacggttct ggaacagacc cagcaggcga cgttcgccaa agctcatgaa
     9061 tgccccacag gtgacaacca ccagcaggat caccaccgct ttgaggatgg tcagcaggat
     9121 ctcaatcagt tccggtgata tccaactcat tgttgtgcct ccttgagatc ctcaagatgc
     9181 gcgccagcca gcaccggagc aatgccggac atacccatcg gcaagcccac ctgccctgcc
     9241 gtcagtcctt cggcgatttc aaccggcagc gtgaccgtgt tgccatcgta actaaaggag
     9301 acgcgtgtac ctgcgttcac acccaacttc gcggcatccg ctgggttgag tttgatgtac
     9361 ggctgcggca tacggctctg gaagaccgga gcacgctgtg acaattcatc gctgccaaac
     9421 aggtggtagt acggcgcgat acgccatttc ccgtcctgcg gctggaagcg tgccggtacg
     9481 ctggtgaagt aatccagacc gttttcgctg gtttcaaaca gacgcacgcc cggatcgcca
     9541 aagcgcagtt tgccgcccac ttcgtcctgg aatttgttcc acgcctgcgg ggagttccag
     9601 cccggcgccc aggcaaacgg cacttgcgaa cggtgcgcgg tcggctggtt gttaccttcc
     9661 atcgagaagg tgaacatggt gtcaatatcc tgcggctgac gcggctcatg aacgctgata
     9721 ttggcgcgca tggcggtacg accgctgtaa cggtgcggtt cacgggccag tttctgccca
     9781 cgaatacgga atgtcgcatc cggcgcagca tctttgatac ccgccagttc cgggattttc
     9841 gccacaacag cgtcaattac atggtcgagc tgcgtccagt ccacttcacg gctcagcaag
     9901 gtgctgtgca gcgagtgcag ccagcgccag ctttccagca tgacagtttt gctgtcgtaa
     9961 taagccggat cgtaaacctg gaagaaacgt tgggcgcgac cttcattatt aattaccgta
    10021 ccgtcgcttt cagcaaagct ggcagaggaa agcaccagat gggcgttttc catgatcgct
    10081 gtgcgttgat gatcaaccac catcaccagc ggcgctttag ccagcgcagc attcacgcgg
    10141 gtagcagaag cgtaacgatg cagatcgttt tccaacacca ccaccgcgtc ggcgcgtccg
    10201 gtttccagtt cggttaacgc ttcttcaagc gaaccgccac ccataatgcc cagccccatg
    10261 ctgttgacgg aacgggcaat catggtgata ccaacgtcag cgccgcgacc tttcagggct
    10321 ttcgcgacgt tagccgcagc ctgaatcacc tctatgctac cggcgttcgt cccggagata
    10381 atcaacggtt tcttcgcacc tgccagtgcc tgcacgatga cgtcgatttt gttttgcagc
    10441 tcaggttcga taccgtcaac cgctggtgcg gagttatcca gcgcatgggc gatggcaaaa
    10501 cctaaacgcg cctgatcttc aaccggtgcg cggtaagtcc acgccgcgat atcatccaga
    10561 cgagtgtcat caacgttggt aacaaacagc ggatgcttcg cacgttgacc gatgttgagg
    10621 attgccgcaa tctgccagtc agccactttc tgtgctgcag ccatttcgcg cgctttacct
    10681 ttcaccgcct gacgcactgc cagtgcgacg cgcgcgccgg tctgggtaac gtcttcgccc
    10741 agcaccagta ccgcatcgta agattcgatt tcgcgcagag ccggagtata aatgccgcct
    10801 tcacgcagca ctttcagcgc cagttgcaga cgttcctgct caccgtgagc gataccggtg
    10861 tagaagtttt cttcgcccac cagttcacgc agcgcaaagt tgctttccac gctggcacgc
    10921 ggagaaccaa taccgatcac tttcttcgac tgacgcagaa tatctgccgc gccctgcatt
    10981 gcctgttcgg cgttgagggt aatgaaatca tcgccacgac gctgtactgg ctgacgcgga
    11041 cgatccttca ggttgacgta accgtaaccg aaacgaccac ggtcgcagag gaagtagtgg
    11101 tttaccgtac cgttgtaacg gttttcgata cgacgcagtt cgccgtaacg ttcaccgggg
    11161 ctgatgttac agccgatgga acattgctgg cagatgctcg gcgcaaactg catatcccat
    11221 ttacggttgt aacgctcgga gtgcgttttg tcggtaaata cgccggtcgg gcaaatttcg
    11281 accaggttac cggaaaattc gctttccagc gtgccgtctt ccgggcgacc gaagtagacg
    11341 ttgtcgtgcg cgccataaac gcccagatct gtaccgtcag cgtaatcttt gtagtaacgc
    11401 acacagcggt agcaggcgat gcagcggttc atttcgtgag agatgaatgg ccccaaatcc
    11461 tgattacggt gggtacgttt ggtgaaacgg tagcgacgga agctgtgtcc ggtcatcacg
    11521 gtcatatcct gaagatggca gttaccgcct tcttcacata ccggacagtc gtgcgggtgg
    11581 ttggtcatta accactcgac cacactttca cggaactgtt tcgcttcttc gtcgtcaatg
    11641 gaaataaagg tgccatcgga agccggtgtc atacaggaca tcaccaggcg accacgcgtg
    11701 tcttccgcgt tttggtattg cttcaccgca cactggcggc aagcaccgac gcttcccagc
    11761 gccggatgcc agcaaaagta aggaatatca aggcccagag acagacaagc ttccagcagg
    11821 ttgtccgctc cgttgacctc gtattctttg ccgtctacat gaattgtagc cattagcatg
    11881 cttccagttt tctcggtcag ggaccgagcg ttaatcgaaa ttcggttacc agcgctcttt
    11941 cagcaggttc ggctgaatcc cattaatcaa atgggtattg ctgaacggct gtttgattcc
    12001 cgcctcaaat tcttcgcgga aatatttgat ggcgctctgt aacggctcca ctgcaccagg
    12061 tgcgtgggca cagaaagttt tacccgggcc taagaatcga cacagttgct caagtgtttc
    12121 gatatcgccc ggctggcctt cgccacgctc cagcgcacgc agaattttca cgctccacgg
    12181 cagaccgtcg cggcacggcg tacaccagcc gcaggactca cgggcgaaaa actcttccag
    12241 gttacgcacc agcgacacca tgttgatctc atggtcaacc gccatcgcca gcgccgtacc
    12301 cagacggctg cccgctttac cgatactttc gaattccatc ggcagatcaa ggtgcgcttc
    12361 ggtcaggaag tcagtccccg cgccgcctgg ctgccaggct ttaaatttca gaccatcacg
    12421 cataccaccg gcgtaatctt cgaggatctc gcgtgcggtg gtgccgaacg gcagttccca
    12481 cagtcccgga tttttcaccc gaccggagaa gcccatcagc ttggtgccag catctttact
    12541 tttcgagatg ttctgatacc actccacgcc gttagcgagg atcgccggaa cgttacacag
    12601 ggtttcgacg ttgttgacac aggtcggttt gccccatacg ccggaggttg ccgggaaggg
    12661 gggtttcgag cgtgggttag cacgacgccc ttccagggag ttgattaacg ctgtttcttc
    12721 cccgcagatg tagcgccctg ccccggtatg gacgaacagt tcgaagtcga aacctgttcc
    12781 cataatgttt ttgccaagca agcccgcttc agtggcttcg gcaatggcgc ggcgcagatt
    12841 tactgccgct tcgatatatt cgccacgcag gaagatgtag ccacggtaag ctttcagcgc
    12901 aaacgcggag atgagcatac cttccaccag caggtgcggc agttgctcca tcaacaggcg
    12961 gtctttatag gtgcccggct ccatttcatc ggcgttacac agcaggtaac ggatgttcat
    13021 ggattcgtct ttcggcatca ggctccactt caggccagtc gaaaagcccg cgccgccgcg
    13081 ccctttcaga ccagcgtctt ttacctgatt aacgatttcg tccggagaca gcccggtcag
    13141 cgccttacgc gccccttcgt aaccgttttt gctgcggtat tcgtccagcc acactggctg
    13201 tttgtcatcg cgcagacgcc aggtcagcgg atgcgtttcg ggagtacgga taatgttttt
    13261 catttatacc gctccagcag ttcagggatc gcttccgggg tcagatgcgc gtgagtgtcc
    13321 tcatcgatca tcatgtttgg ccctttatca cagttcccca ggcagcaagt tggcagcagc
    13381 gtaaagcggc catcaaatgt cgtttgccct ggtttgatgt tcagcttttt ctcgagcgcc
    13441 gcctgaatac cctgataccc gttgatatga cagaccacgc tgtcgcaata acggatcaca
    13501 tggcgaccaa ccggctggcg gaagatctga ctgtagaacg ttgccacacc ttcgacgtcg
    13561 cttgccggaa tacccagcac atcggcgatc gcgtggatcg caccatccgg cacccagcca
    13621 cgctgcttct gaacgatttt cagcgcttca atggacgccg cacgcgggtc ttcgtagtgg
    13681 tgcatctcgt gctcaatcgc ttcacgctct gccgcactca gctcaaaagc ctcggtttgt
    13741 ggttgttgat tctcgtgcat aattagcggt ccacatctga cataacaaaa tcgatactgc
    13801 ccagataaac aatcaggtca gacaccaggc tgccgcggat cgccgccgga atttgctgca
    13861 aatgcgcata actcggcgtg cggatacgag tgcgataact catggtgcta ccgtcgctgg
    13921 tcaggtagta actgttgatc cccttggtcg cctcaatcat ctggaaagat tcattggcag
    13981 gcatcaccgg cccccacgac acttgcagga agtgggtgat cagggtttcg atatgttgca
    14041 gcgtgcgctc tttcggcggc ggcgtggtca gcgggtgatc cgctttgaac gggccttccg
    14101 gcatgttgtt gaggcactgc tcaagaatgc gcagactctg gcgcagctct tccactttca
    14161 gcattacgcg ggtgtagcag tcagaaacac caccaccaac cgggatttca aagtcgaagt
    14221 tttcatagcc agaataagga cgcgccttac gcacgtcgaa gtcgatcccg gtagcacgca
    14281 ggcccgcgcc agtggtgccc cactccagcg cctctttcgc gccataggcg gcaacgccct
    14341 gggaacgacc tttcagaatg gtattttgca gcgccgcttt ctcgtaagac gccagacgtt
    14401 tcggcatcca gtcgaggaac tcacgcagca gacgatccca gccgcgcggc aggtcgtgcg
    14461 ctacgccgcc aatacggaac cacgccgggt gcatacggaa acccgtgatc gcttccacca
    14521 gatcgtaaat tttctgacga tcggtaaagg cgaagaacac gggcgtcatt gcgccgacgt
    14581 cctgaataaa ggtcgagatg tacagcaggt ggctgttgat acggaacagt tcggagagca
    14641 taacgcgaat gacgttaacg cgatccggca cggtgatccc ggccagtttc tctaccgcca
    14701 gcacgtaagg catttcgtta acgcagccgc cgaggtattc aatacggtcg gtatacggaa
    14761 tgtagctgtg ccaggactgg cgctcgccca ttttctccgc accacggtgg tggtagccga
    14821 tgtctggtac gcagtcgaca atctcttcgc cgtcgagttg taaaacgata cggaaagccc
    14881 cgtgcgccga cgggtggttc ggaccgaggt tgaggaacat gaagtcctcg ttttcggtgc
    14941 cgcgcttcat cccccactct tccggtttga aggtcagggc ttccatctcc agatcctgtt
    15001 tggctttggt cagctcaaac ggcgagaatt cggtagcgcg cgccgggtaa tctttacgca
    15061 gcgggtgacc tttccaggtt tgcggcatca tgatgcggcg caggttcggg tgaccgtcga
    15121 aagtaatgcc aaacagatcc caggtttcac gctcatacca gttagcgttc gggaacagtt
    15181 tggtgaaggt cggtacgtgc aggtcgtttt ctgccagcgc caccttcagc atgatgtcgc
    15241 ggttacgatc gatagaaatc agatggtaga aaacggaaaa atccgcggca ggtaaccctt
    15301 cgcggtgtgt gcgcagacgt tcgtccatgc cgtgtaagtc aaacagcatg acgtaaggtt
    15361 tcggcagttt ctttaagaaa tcgccaactt ccagtaattg ttcacgcttg atccacacaa
    15421 cgggaacccc ggtgcgagtc gcctgaacag taaaggcatc cggcccaaaa cggttgcgca
    15481 gttcgccaat taccggatca tcaagatgat cgcgggtctg ccaggcgggt tcttgcgcgg
    15541 ttaagtcggt catattgttc accattgcaa atggtccgtg gtgactgtcg gcgcagtaag
    15601 cttcgcgaaa tagatttagg aataagcgaa gaaaaatccc tttgccgaca ggcgcaaatt
    15661 aaatctcgtc aggtgtacgc aggttggtta cggcaatgcg ttcaccgcgc ttgcgttcgc
    15721 gctctgattg catattggcg cgataaacgc cctgatcgcc aaccacccag gagagcggac
    15781 gacgttcttt gccgatagat tcctgcaaca gcatcagtgc ctgcatgtac gcttcaggac
    15841 gcggcgggca gcccgggata tacacatcaa ccgggatgaa tttatcgacg ccctgcacaa
    15901 cggaataaat atcgtacata ccaccagagt tggcacaggc acccattgag ataacccatt
    15961 ttggttccag catctggtca tacagacgct gaataaccgg tgccattttg gtaaagcagg
    16021 ttcctgcaac caccatcagg tcagcctgac gcggcgaagc acgcaatact tctgcgccaa
    16081 aacgcgccac gtcatgcacc gcggtaaacg aagtcaccat ctcaacgtaa cagcaggaaa
    16141 gaccgaagtt atacggccaa attgagtttt tacgacccca gttaaccatg tcattgagct
    16201 tgcccataaa cacgtttttg ttaacttctt gctccagagg gtcggttacg atctcctgct
    16261 tttgcagggg gtaacggtcg ttctcaccgt tgggatctat gcgggtgagc gtataatcca
    16321 tcttaatgcc tcgcggttag cgttgacgat tagcgatact gttcgtttcc gggttcatac
    16381 gctcgcggcg tgaacgcgcg ggcgtccagt ccagcgcgcc aatacgcacc agataaacca
    16441 gacctgccag taacacaaaa ataaaaattg cagcttccac aaagcctacc cagccgcttt
    16501 cgcggataga ggttgaccat gcgaacagat acagcgcttc aacgtcgaag ataacgaaga
    16561 acatggccac cagataaaac ttggcggaca ggcgtaagcg ggcggagccg accgagtcga
    16621 taccggattc aaacggcacg tttttcgacc tcgcgcgtgc gcgaccgcct aaaaaccaac
    16681 cgcctaccag catcaggcaa cacaggccaa tggcaacgat aagaaagata gcgaatgccc
    16741 agtgatgagc gatgacttca gtggatgttg acatactcat tgcttactca tcaaaagtag
    16801 cgccagattc actgctcttc acggcagatg gacgccacat cgattcatgg ggaggaataa
    16861 aaaaaacctt acaataactg tagaaattct tttatacagc taattgatgt ggtcttttac
    16921 tcctttctat aaccttttgt caactttaac aaaagcttct tcacattagt ttacataata
    16981 tcaacaccat tagcatttaa tgccctttca ccccagatcc ttgacgactc caggataatt
    17041 agatgttgtt gaatcgtgtc cgttgtgaag caatggaaaa aatacgggtc tattttgaca
    17101 ggaatttgtg tcgattcctc cccccaaaag agagtatttt cttgatctgt gacacgcttt
    17161 tgttattcca taacaaaaac gcagcaacaa atttacgtat tttttaacat cattgtagca
    17221 ggtgattttt ttcaggcgat tatttgtgca ttcgggacgt gaatctctgg tgattgaaaa
    17281 ataaacagtt ttgtacgttc tgcactatgc gaggaaggat ttttactaaa aaaaagccgc
    17341 tggggtttaa aacaccccca gcggctcgtt ttttacacta ttgtctcagg aattatctat
    17401 cgtccgtcga ttactcgata tccctttcaa tcaacaatga atcatcccct tccggagcag
    17461 acattgggct gtattgccac ggattatggt agctctccat cgcctgatag atcacctggg
    17521 ccagctcatt attactggac gggtcatagc acagcaaata ttcggtatca ggtagcggcg
    17581 gtaagccatc tactccactc agcacacgca ggtccgggct catcatctca accggccttg
    17641 ccgtcacgcc aagaccggct ttcactgccg cacgaacggc cggaagcgtc gaggcgacat
    17701 aagccagtcg ccatggaata tctactttat taagcgtcgc cagcaccata tcgcgaaacg
    17761 gacttggatc atccagcaat acaagaggga tcggctcgcc tttttgcaat acgtattccg
    17821 ctgcgcagta ccagtgtgtt ggcgaggtac gcaggttcaa tgccttaaac gctgaaggac
    17881 gatgggtggt taccatcaaa tccacttcct gggattccag catttctgcc atataggcat
    17941 tacgtttaac gcggacatcc agcgctaatt tcggataaac cgaactcacg cgatttaaca
    18001 ggaaaggtaa gatcgtatcg gcagattcat ctgaagcacc gatagttaac acgccctgaa
    18061 gattactgaa cattaatgat gagcaggcct catcattaaa acgcaggatt ttcctggcgt
    18121 aaccaagaag ttgaatgcca tgttcagtta acagtttgtt gcgaccgtga cgagcgaaca
    18181 gttctttccc aacgagttgt tccagacgct gcatttgctg acttacggcg gactgagtac
    18241 gacacacagc ggcagctgcg gcagcaaaag tgttcagatc ggcaacagca acaaatgttc
    18301 tcagcagatc gaggtcgagg ttaattatcg gacgatttgc acttatcata tattatcact
    18361 tactggcggc tcatactgag ctggttaatg ctgtgcacac acaaacaagc aattccattt
    18421 gtaatgtgcc tccctggcag tttcatccgg aacccggacg aaagtaaaaa tgcatatgag
    18481 ttgcactaaa aaagcgactc acattgttcc gttataatgc ctgaagtaga tcacagaata
    18541 tatcttcagg gatcgcatat ctattaagtt actcactctt ttctatttat gacatgcgcg
    18601 tgtttgtata aatgtaaatg tgagtccttg ttccactctc gtgcagcatc gctggtcata
    18661 cgcgaacacg tacaaacagc aatggtgaag cggcatcaag agcagggatc cgtcatttat
    18721 gcgagcattt taccccaaaa accttttatt tataagggtc attgcgaatt atctgatgca
    18781 aagttatgtt atgttaggca aagtaatctt ctattattaa taagcacatc aaaacctttt
    18841 tgaatattaa ataataatta attagcatca tcctcattca ttaattccgc ttaacaatag
    18901 tttcacacct tacccccatt gcgacacctc ggaaatcatc acgctgttaa actctccgta
    18961 atgcggaaat atcatcatcg cgtttcatta gtgaattctt aaatgaggca ttttcacaca
    19021 attatcttac agataaaaaa accagacttg caattaagaa ttagaacaat tcactatata
    19081 acattgcatg taaagcatat aaacctcatt attttgtcat tattaagtta ttaacagcac
    19141 aatcgagcct tcccctctgg caaaatctta ttctgcagac cttcaaaaca ccgtcctggg
    19201 ggagtacatt gttctaagct gacttccacg gcagggagtg gcgataacag caaaaaaggt
    19261 caagattcat gtcccccatt gaaaaatcca gcaaattaga gaatgtctgt tatgacatcc
    19321 gtggtccggt gctgaaagaa gcaaaacgcc tggaagaaga aggtaacaag gtactgaaac
    19381 tgaacatcgg caacccagcc ccattcggtt ttgacgcgcc agatgaaatc ctcgttgacg
    19441 tgatacgcaa cctgcctacc gctcaagggt attgcgattc caaagggctt tactccgcgc
    19501 gtaaagccat catgcagcac taccaggctc gtggcatgcg tgatgttacc gtggaagata
    19561 tttacatcgg caatggtgta tcggagctta tcgttcaggc aatgcaggca ttgctgaaca
    19621 gcggggacga aatgttggtt cctgcaccag attacccact ctggaccgcg gcggtttcgc
    19681 tttccagcgg taaagcggtg cattatcttt gcgatgaatc ctctgactgg ttcccggacc
    19741 tcgatgatat tcgcgctaaa attacgcctc gtacgcgtgg gattgttatt atcaacccaa
    19801 ataacccaac cggcgcggta tattccaaag agcttttaat ggagattgtg gagattgcac
    19861 gtcagcataa tctcattatc ttcgccgatg aaatttatga caaaattctc tacgacgatg
    19921 ctgagcatca ctcaattgcg ccgctggcac ctgacctgct gaccattacc tttaacggac
    19981 tgtcgaaaac gtaccgcgtt gcaggcttcc gtcaggggtg gatggtgttg aacgggccga
    20041 aaaaacacgc caaaggctac atcgaaggtc tggaaatgct ggcttcaatg cgcctgtgtg
    20101 ctaacgttcc tgcgcaacac gccattcaga ccgcgctggg tggttatcag agcatcagtg
    20161 aatttattac ccctggcggt cgtctttatg agcagcgtaa ccgcgcgtgg gaactgatca
    20221 acgatattcc gggcgtttcc tgcgtgaaac ctcgtggtgc gctgtatatg ttcccgaaaa
    20281 tcgacgccaa acgctttaac attcatgacg atcagaaaat ggtgctggat ttcctgttgc
    20341 aggaaaaagt tctgttggtg caagggacgg cattcaactg gccgtggccg gatcacttcc
    20401 gcattgtcac gctaccgcgt gtcgatgata tcgagctgtc tttgagcaag ttcgcgcgtt
    20461 tcctttctgg ttatcatcag ctgtaatctt aatttcactg ccggagattg catccggcag
    20521 tgttatcccg ccacaatgac ctgatgatgt catcatacgt aaggtcacta tgaaacagag
    20581 ccattttttt gcccatctct cccgcctgaa actcattaac cgctggccgc taatgcgcaa
    20641 cgtgcggacg gaaaatgtgt ccgaacacag tttgcaggta gcgatggtcg cccatgcgct
    20701 ggcagctatc aaaaatcgga aatttggcgg taatgtcaac gccgaacgta tcgctttact
    20761 ggcgatgtac cacgatgcct cagaagtgct caccggcgat ctccctactc cggtgaaata
    20821 cttcaatgcg caaatcgctc aggagtacaa ggctattgaa aaaatcgctc agcagaaact
    20881 ggtcgatatg gttccggaag aactgcggga tatctttgcg ccgttaattg acgagcatgc
    20941 atatagcgat gaagaaaaat cgctggtgaa acaggcagat gcactgtgtg catatctgaa
    21001 atgtctggaa gaactcgcgg ccggaaataa tgaattcttg ctggcaaaaa cgcgactgga
    21061 agcgacgctt gaagcgcgtc gcagccagga gatggactac ttcatggaag tatttgttcc
    21121 cagcttccat ctttcgctcg atgagattag ccaggattca ccgctgtaag cagccggagt
    21181 ctgcgtcgca tcagtcaata agcgccggat gcgacatcag gctcttgtca aaacggaaac
    21241 agcatcggga tcatcaccac acaaaccgcc atcacgataa tggtgaacgg tacccccaac
    21301 ttcacaaagt cactgaagct gtaatttccc ggacctaaaa ccagggtgtt aacaggtgaa
    21361 gacaccggtg tcataaaggc ggcggatgct gccatcgcca cgaccatcgc aaatggataa
    21421 ggcgacactc ccatcgtttt ggcagcagcc agcgcaatcg gagccatcaa caccgccgtc
    21481 gcggtattag agataaacag cccaataacc gccgacaaga caaacaaaca ccccagcatc
    21541 atatgtggcc cataaccgcc gccaatatcc atcagccctt tcaccgccag cgcgacacct
    21601 cccgttttct gtaatgccac agcaaacggc atcatcccaa cgatcaaaat aatgctcggc
    21661 cagtgaatgg atttataggc gctttcagca tctatacagc ggaatttccc catcagcagg
    21721 caggcgatga tagcggcaac aggattagga atttcatttg tcagcattaa cgccaccatt
    21781 agcaccagac agaaaatggc atggggtgcc tggctgtgcg cgggcgatgc ttcactcacc
    21841 tcttccggta agttcagcgc tacgaagtcg cggccctgtt tggccagcat accgatcagt
    21901 ttccagttac ccacaaccag gatgatatcg cccagcagca gaggctcatc cgccagcgaa
    21961 ccttccagcg ccacgccatt gcgcttgagc cccaccacat tcagtccgta gcgggtacga
    22021 aaaccaattt cgcgcaccga tttaccaatc agttctgact caggaattaa tgaaatctct
    22081 gccatgccca catcaagggc ctggtcagaa aaatactcgc cgcgcagtac catcggctcc
    22141 agcaattgct cactacaaaa ttgccggaga tcgacatcag ccgcagacat atcaataagc
    22201 aaaacgtcac gcgcgcgaaa ttcagaaacc ccattaacgt tcacgataac gcgacgaaaa
    22261 cgccgccagc gttcaacacc gatgacgtta gcgccataac gctcacgtaa tttgagatca
    22321 tccagccgtt gacctatcat tggcgatccg gggcgaatag ccagacgtcg cgcacgcccg
    22381 gtcagtcgat attcacggat aagatcgcga aaggttcgac gcgtccagcc ttcgcgctgc
    22441 ggggtctggg tatccccttt cagcatgaaa cgcatcacta acatatacaa aatacccagc
    22501 accagcacga ccaggccaat aggtgttacg ctaaagaaac tgaagccgtg atagccttca
    22561 cgcagcaatt cactgttgac taccaggttc ggcggcgtcg ccaccagcgt catcatgccg
    22621 ctaatcagcc cggcaaaact taacggcatc atcagacgtg acggcgaagt ttgcatacgc
    22681 atggcaacgc ttaacaccac gggaataaag atagcgacaa cgctggttga actcataaac
    22741 gcgccaagcc ccgcgacggt cagcatcaac aaaaccaaca ttttgatttc actgttgccc
    22801 gccactttga ccagccatgt tcccattacg gtggcaacac cggtacggac caaaccatcg
    22861 ccaataataa acaaggcggc aatcaggaca acgttaggat cagaaaagcc ggaaaatact
    22921 tcggggaccg tcagcgttcc gcttaatgca aacgcgacaa taacaaacaa agcgaccgca
    22981 tccatacgca ctctgcccgt cgcaaacaag acgatggcaa cggccagtaa tgaaagaacc
    23041 caaatcaatt caccgttcac aacttatcct tgttaattga gggggatgac ttgattctgc
    23101 cataaaaaag ccccgacgag acggggctaa atcatgatca ggtgtttcac tgaataataa
    23161 catcgccatt tggctgtttg gtcacagtaa tttgctccag actgtggagg accaaatcga
    23221 cctcattcag gcgcggggta tctgccggag cgttaaccgc aatgacatgg caacccgccg
    23281 ccaggccaga aagcacgcca gcgggagcat cttccaccac cacacactcc tgcggcgcaa
    23341 gccccagcag ctgcgcacct aacagatacg catcaggttc tggttttccg cgcttcactc
    23401 gctcagcggt tacaaacacc tctggtgcgg gaagccccgc tattttatgg cgcgctcgcg
    23461 ctaccggcat ggagccagaa gtcacaatgg cccacggaat acctgcttta ttcaaatgat
    23521 tgagtaaggc gattgcccca ggaagcgcgg taataccttc ggtttccgtg gcctcgattt
    23581 gctccagacg cgtaaactcg gcggcaatat cagcctcgga tttgcccgcc ataaaatggc
    23641 gcaaagaggt gatcgcctgt ttaccgtgaa tgaaagccag cacctcttcc ggcgctaacc
    23701 catgacgtct ggcccagttg ctccacgccc gttctaccgc aggcagggaa tccaccagcg
    23761 ttccatcaag atcaaacaga aaacctttgc accgcacgcg ggcctcctca ggcgttgata
    23821 atttgattaa tttcgttggc gctcaaatgg tactgacgcg ggcaggcatg ccacacatta
    23881 agcatgcgct gatatttttc ccacattggc gtctgggcgt taaaaccatg agtcccagcg
    23941 tcaaaatggg tatagcgccc ttccacatta accataaagc ggacataacc gaggtaacgt
    24001 gcttcagtgg cggcgtcaaa gccgaggaag gtgacacgac gttcatcgat ggattgctgg
    24061 tcctgcaaat tagaccagga aacatacaac gcatgataca tctccataat gtcgatgata
    24121 gtgcggcagg tttcttcttt cagctcgcca aactcgcgat ccaattcacg catctgtaat
    24181 ccgtaaccac gctcaataat tgtttgcagg cgacggtaac gttcagcatt tgccggatcg
    24241 agcatagtca tcatcttgta ctgattagac aaaataagac gttgcgcgtt ggtcatttcc
    24301 attgttgact cctgtatcac tctactacgg tgaaaaaaaa gaaggctgag tatgccttct
    24361 tttatatgcg taatcagggg tcaattacaa atcatcaagg aaagttttat ccagttgttt
    24421 gaaggcgcgc ttaagcgtgt cagctaatgc ctggtaatca ggcttgcctt caacgggtgc
    24481 caacacctgt ccagactcct gcaatttacc gcgaacttca taaaaccagt taaggattgc
    24541 agggggtaat ggcgttacag aacgcttgcc cagccaccac aatccctgca tgggtaaact
    24601 taaggcgaac agtgcagtgg caactgccgg cccaagcaga ccgcccaggg caatctgcca
    24661 gcagagagta aatacggcga tcggcggcat aaaacggatc gcataacgcg tcatcttgat
    24721 aacgcgattt tcgacaaaga ccggggcaag gcgtttttcc agtggccacg tctttgagta
    24781 atgctgtccg cggcgaaaca agctaaaaaa attaacagaa cgattatccg gcgttgacat
    24841 gcttcacctc aacttcacat ataaagattc aaaaatttgt gcaaattcac aactcagcgg
    24901 gacaacgttc aaaacatttt gtcttccata cccactatca ggtatccttt agcagcctga
    24961 aggcctaagt agtacatatt cattgagtcg tcaaattcat atacattatg ccattggctg
    25021 aaaattacgc aaaatggcat agactcaaga tatttcttcc atcatgcaaa aaaaatttgc
    25081 agtgcatgat gttaatcata aatgtcggtg tcatcatgcg ctacgctcta tggctccctg
    25141 acgttttttt agccacgtat caattatagg tacttccatg tcgagtaagt tagtactggt
    25201 tctgaactgc ggtagttctt cactgaaatt tgccatcatc gatgcagtaa atggtgaaga
    25261 gtacctttct ggtttagccg aatgtttcca cctgcccgaa gcacgtatca aatggaaaat
    25321 ggacggcaat aaacaggaag cggctttagg tgcaggcgcc gctcacagcg aagcgctcaa
    25381 ctttatcgtt aatactattc tggcacaaaa accagaactg tctgcgcagc tgactgctat
    25441 cggtcaccgt atcgtacacg gcggcgaaaa gtataccagc tccgtagtga tcgatgagtc
    25501 tgttattcag ggtatcaaag atgcagcttc ttttgcaccg ctgcacaacc cggctcacct
    25561 gatcggtatc gaagaagctc tgaaatcttt cccacagctg aaagacaaaa acgttgctgt
    25621 atttgacacc gcgttccacc agactatgcc ggaagagtct tacctctacg ccctgccgta
    25681 caacctgtac aaagagcacg gcatccgtcg ttacggcgcg cacggcacca gccacttcta
    25741 tgtaacccag gaagcggcaa aaatgctgaa caaaccggta gaagaactga acatcatcac
    25801 ctgccacctg ggcaacggtg gttccgtttc tgctatccgc aacggtaaat gcgttgacac
    25861 ctctatgggt ctgaccccgc tggaaggtct ggtcatgggt acccgttctg gtgatatcga
    25921 tccggcgatc atcttccacc tgcacgacac cctgggcatg agcgttgacg caatcaacaa
    25981 actgctgacc aaagagtctg gcctgctggg tctgaccgaa gtgaccagcg actgccgcta
    26041 tgttgaagac aactacgcga cgaaagaaga cgcgaagcgc gcaatggacg tttactgcca
    26101 ccgcctggcg aaatacatcg gtgcctacac tgcgctgatg gatggtcgtc tggacgctgt
    26161 tgcattcact ggtggtatcg gtgaaaatgc cgcgatggtt cgtgaactgt ctctgggcaa
    26221 actgggcgtg ctgggctttg aagttgatca tgaacgcaac ctggctgcac gtttcggcaa
    26281 atctggtttc atcaacaaag aaggtacccg tcctgcggtg gttatcccaa ccaacgaaga
    26341 actggttatc gcgcaagacg cgagccgcct gactgcctga tttcacaccg ccagctcagc
    26401 tggcggtgct gttttgtaac ccgccaaatc ggcggtaacg aaagaggata aaccgtgtcc
    26461 cgtattatta tgctgatccc taccggaacc agcgtcggtc tgaccagcgt cagccttggc
    26521 gtgatccgtg caatggaacg caaaggcgtt cgtctgagcg ttttcaaacc tatcgctcag
    26581 ccgcgtaccg gtggcgatgc gcccgatcag actacgacta tcgtgcgtgc gaactcttcc
    26641 accacgacgg ccgctgaacc gctgaaaatg agctacgttg aaggtctgct ttccagcaat
    26701 cagaaagatg tgctgatgga agagatcgtc gcaaactacc acgctaacac caaagacgct
    26761 gaagtcgttc tggttgaagg tctggtcccg acacgtaagc accagtttgc ccagtctctg
    26821 aactacgaaa tcgctaaaac gctgaatgcg gaaatcgtct tcgttatgtc tcagggcact
    26881 gacaccccgg aacagctgaa agagcgtatc gaactgaccc gcaacagctt cggtggtgcc
    26941 aaaaacacca acatcaccgg cgttatcgtt aacaaactga acgcaccggt tgatgaacag
    27001 ggtcgtactc gcccggatct gtccgagatt ttcgacgact cttccaaagc taaagtaaac
    27061 aatgttgatc cggcgaagct gcaagaatcc agcccgctgc cggttctcgg cgctgtgccg
    27121 tggagctttg acctgatcgc gactcgtgcg atcgatatgg ctcgccacct gaatgcgacc
    27181 atcatcaacg aaggcgacat caatactcgc cgcgttaaat ccgtcacttt ctgcgcacgc
    27241 agcattccgc acatgctgga gcacttccgt gccggttctc tgctggtgac ttccgcagac
    27301 cgtcctgacg tgctggtggc cgcttgcctg gcagccatga acggcgtaga aatcggtgcc
    27361 ctgctgctga ctggcggcta cgaaatggac gcgcgcattt ctaaactgtg cgaacgtgct
    27421 ttcgctaccg gcctgccggt atttatggtg aacaccaaca cctggcagac ctctctgagc
    27481 ctgcagagct tcaacctgga agttccggtt gacgatcacg aacgtatcga gaaagttcag
    27541 gaatacgttg ctaactacat caacgctgac tggatcgaat ctctgactgc cacttctgag
    27601 cgcagccgtc gtctgtctcc gccagcgttc cgttatcagc tgactgaact ggcgcgcaaa
    27661 gcgggcaaac gtatcgttct gccggaaggt gacgaaccgc gtaccgttaa agcagccgct
    27721 atctgtgctg aacgtggtat cgcaacttgc gtactgctgg gtaatccggc agagatcaac
    27781 cgtgttgcag cgtctcaggg tgtagaacta ggtgcaggca ttgaaatcgt tgatccagaa
    27841 gtggttcgcg aaagctatgt tggtcgtctg gtcgagctgc gtaagaacaa aggcatgacc
    27901 gaaaccgttg cccgcgaaca gctggaagac aacgtggtgc tcggtacgct gatgctggaa
    27961 caggatgaag ttgatggtct ggtttccggt gctgttcaca ctaccgcaaa caccatccgt
    28021 ccgccgctgc agctgatcaa aactgcaccg ggcagctccc tggtatcttc cgtgttcttc
    28081 atgctgctgc cggaacaggt ttacgtttac ggtgactgtg cgatcaaccc ggatccgacc
    28141 gctgaacagc tggcagaaat cgcgattcag tccgctgatt ccgctgcggc cttcggtatc
    28201 gaaccgcgcg ttgctatgct ctcctactcc accggtactt ctggtgcagg tagcgacgta
    28261 gaaaaagttc gcgaagcaac tcgtctggcg caggaaaaac gtcctgacct gatgatcgac
    28321 ggtccgctgc agtacgacgc tgcggtaatg gctgacgttg cgaaatccaa agcgccgaac
    28381 tctccggttg caggtcgcgc taccgtgttc atcttcccgg atctgaacac cggtaacacc
    28441 acctacaaag cggtacagcg ttctgccgac ctgatctcca tcgggccgat gctgcagggt
    28501 atgcgcaaac cggttaacga cctgtcccgt ggcgcactgg ttgacgatat cgtctacacc
    28561 atcgcgctga ctgcgattca gtctgcacag cagcagtaat ctcgtcatca tccacagctt
    28621 tgcgctgcgg atatctgaac cggaaataat cactatttcc ggttttttat tctcttaatt
    28681 tgcattaatc ctttctgatt atcttgctta actgcgctgc atcaatgaat tgcgccatct
    28741 cactttgcat acttaccact ttgttttgtg caagggaata tttgcgctat gtccgcaatc
    28801 actgaatcca aaccaacaag aagatgggca atgcccgata cgttggtgat tatctttttt
    28861 gttgctattt taaccagtct tgccacctgg gtagttccgg tggggatgtt tgacagtcag
    28921 gaagtgcagt atcaggttga tggtcaaaca aaaacacgca aagtcgtaga tccacactca
    28981 tttcgcattc tgactaacga agcaggcgaa cctgagtatc accgcgtaca gctgttcacg
    29041 acgggcgatg aacgcccggg cctgatgaac ttcccgtttg aagggttaac ctcaggatcg
    29101 aaatacggga cagccgttgg catcatcatg tttatgctgg tgattggcgg cgcgtttggc
    29161 attgtgatgc gtacaggaac cattgataac ggtatcctgg cgcttattcg ccatacccgc
    29221 gggaatgaaa ttctctttat tcctgcgctg tttattctgt tttcacttgg cggcgcggta
    29281 tttggtatgg gagaagaggc cgtcgccttt gccattatca tcgcaccgct aatggtccgg
    29341 ctgggctatg acagtattac caccgtcctg gtgacttata ttgccacgca aatcggtttt
    29401 gccagttcgt ggatgaaccc gttttgtgtg gttgttgctc aggggattgc cggcgttccg
    29461 gtgctttctg gctccgggtt gcgcatcgtg gtgtgggtta tcgccactct gattggcctg
    29521 atctttacca tggtgtacgc ctcacgagtg aaaaagaatc ctcttctgtc acgcgtgcat
    29581 gagtccgacc gcttctttcg tgaaaagcag gcggatgttg aacaacgtcc gtttaccttt
    29641 ggtgactggc tggtattgat tgtcctgaca gccgtaatgg tctgggtgat ttggggcgtg
    29701 atcgttaatg cctggtttat tccagaaatt gccagccagt tcttcaccat gggtctggtg
    29761 attggcatca tcggcgttgt tttccgcctt aacggcatga cggttaatac catggcttca
    29821 tcctttaccg aaggggcgcg aatgatgatc gcccctgccc tgctggtggg tttcgccaaa
    29881 gggattttgc tgctggttgg taatggtgaa gcaggtaatg ccagcgtgtt aaatactatc
    29941 ctcaacagca ttgccaatgc cattagcggt ctggacaacg cggtcgcggc ctggtttatg
    30001 ttgctcttcc aggctgtatt taatttcttc gtgacgtccg gttctggtca ggcggcatta
    30061 actatgccgt tactggcacc gcttggcgat ctggtcggtg ttaaccgtca ggttaccgtg
    30121 ctggcgttcc agtttggtga tggcttcagc cacatcattt acccaacctc agcttcgtta
    30181 atggcaacgc tcggtgtgtg ccgggtggac ttccgtaact ggctgaaggt gggcgcgacc
    30241 ctgcttggac tgctgtttat tatgtccagc gtcgtagtga tcggcgctca gttgatgggc
    30301 taccactaaa aatgttaaga gccgcaattg cggctctttt ttcattctgc cgtttcagtc
    30361 tctacggctt catttttggc attgcgcttc atccacaacg ccagcgcttt cagcgagtct
    30421 ggagtgaact catcgcagcg tgcggtgatt tcttccggcg tcagccagca aactccactg
    30481 acttcatctt cctgtagggc gaagggaccg tgagagacgc aactgaacaa cgcgccccag
    30541 acacggcaat ttttatcttc gaaatagaac tgcccgtgct cggcaaaggg gacaccggca
    30601 atgcccaact cttcttccgc ttcgcgacgc gcggattcca gcagttgctc atcggcctga
    30661 actacaccgc ctgcggtcgc atctaacatg ccgggtaaaa agtcttttgt ctcggtacga
    30721 cgctggacca gaattttgcc catgccatca tgcacgacga tgtaagttgc acgatgacgc
    30781 agacactgtg cccgcatttg ttcccggctg gcttgtgcaa tgacttcgtt ctcttcattg
    30841 acaatatcca cccattcagt acttgccaaa cgacgctgtt ccattatcag gaaaccttct
    30901 ttttctggcg ctcttacggc gcattttgag ttgtgggtaa attacggatt aatcgcgacc
    30961 tgcgcaatga tactttgatc attgagtgcg attacactaa gtacgtcatt atccagcatg
    31021 ccataacccg ccggattacc gcctttcgga atactcaccg agccggggtt gaagtggaaa
    31081 atctcccccc gctgttctgc cactggcaga tgggtatgac cgtacaccag cacatcgttc
    31141 tggtttaaag caggtagatt ttccgggcca aaaagatgac catgcgtcaa aaacagacgt
    31201 tgtttttcca gtaatacctg ttgccacggc gcggttatcg ggaaatgcag catcatttga
    31261 tccacttcgc tgtcgcagtt gccacgcaca gcgataacct tatgtgccac ttcattaagc
    31321 cgttcagcga ctttggctgg cgcgtaaccc tccggtaaag cattacgcgg gccgtgattc
    31381 aacacgtcgc caaggatcac cagccactgg gcaccgcttt ggacaaacaa ctccacaaca
    31441 cgttccgtcg ccggtaacga cccatgaatg tccgatgcaa acatcagttt catcactcac
    31501 tcctcgtcga aaaaacgtaa gctatgatac tggattctgt cgttgctatc agccagattg
    31561 cttcgccgaa agtgcaataa aacgctggac agacgctcgc tgccactgga atgtcgcata
    31621 atccaccagg cgttctggca cctcatcgcc atgtagtacc aggcggttaa tcattagcgc
    31681 cagatcagta tcagcaatgc accattcacc aaataaattc ggctgaccca gtgctaacaa
    31741 atgttctgcc atcgcgaaca gtttctctgc actggctttt ccctcggccg ttagtggcgc
    31801 ttttttcgcc cccgcaaaga caacatccgt cggacgctct tcgcggatgg gcatcagatc
    31861 gctgcgcagc caggcctgaa tctgtcgtgc acgcgcacga ttttctaaat caagcggata
    31921 aatacgttcc caggtcggtg gcgcaaatcg atcttccaga tactccgcaa tggcagaaga
    31981 ttcactcaac tcaaaatcat cgatttgtaa taacggcaca cggcgtgtct gaccgtaacc
    32041 ttgccacgtc ggctgcaaat gttcaccgct gtcgaggtcg atggtcttga tatgaaacga
    32101 caggcctttt tcctgcaacg ccacccaggc ggataacaca taaggggaga aaaagtgggc
    32161 atctgaccaa agcgtgatag cgggtttact cattatgtcc tcggcaataa agggggctgg
    32221 ctatcaaact atagagcctt tttgtcactg tcacgtgacg aatactcacg taagtcgccg
    32281 cgctttctat acttgtggtg ttcagatgat taaacaaccg gagctgcaat gatcgatctc
    32341 tatttcgccc cgacacccaa tggtcacaaa attacgctgt ttctcgaaga agcagagctt
    32401 gattatcgct tgattaaggt agacctgggg aaaggcggtc agtttcgtcc ggaatttttg
    32461 cgcatttcgc ccaacaacaa aattccggca attgttgatc attctcctgc cgatggcggc
    32521 gaaccgttaa gcctctttga gtctggtgcc attttgttgt atctggctga gaaaacagga
    32581 ctctttttga gtcatgaaac gcgtgagcgc gccgccacat tacagtggtt attctggcag
    32641 gtaggcggac tggggccgat gcttgggcaa aatcatcatt ttaatcacgc agccccccaa
    32701 accattcctt acgctattga acgttatcag gttgaaactc agcgtcttta ccatgtacta
    32761 aacaagcggc tggaaaactc gccctggctg ggaggcgaga actacagcat tgcggatatt
    32821 gcctgctggc cgtgggttaa tgcctggact cgcctgcgaa ttgacctcgc aatgtatccg
    32881 gcagtcaaga actggcatga gcggatccgt tcgcgccctg ccaccgggca ggcactgcta
    32941 aaagcacaac tcggtgatga gcgttcggat agttaacaga aacaggttct cgtgtattat
    33001 ttcatcctaa gtaaaacaac ggagaacctg caatggcaca acctgccgct attattcgta
    33061 taaagaacct tcgtttgcgt acgtttatcg gaattaagga agaagaaatt aacaaccgtc
    33121 aggatattgt tatcaatgtg acgatccact accccgccga taaagcgcgc actagcgaag
    33181 atatcaacga tgcgctgaat tatcgcaccg taacgaaaaa cattattcag catgtagaga
    33241 ataaccgttt ctctttgctg gaaaaattaa ctcaggatgt gctcgatatc gcacgtgaac
    33301 atcactgggt gacgtatgct gaagtggaga tcgataaact gcacgcgctg cgctacgccg
    33361 attcggtatc catgacctta agctggcagc gttaatcgcc atatcgggag gctgcatgaa
    33421 catagtgatc accggaggga cgggattaat tggtcgccat ttgattccgc gtttgctgga
    33481 gctgggccat caaattacgg tagtgacgcg taacccgcag aaagccagtt ccgttctcgg
    33541 ccctcgggtg acactatggc aagggcttgc cgatcaaagc aacctcaacg gcgttgatgc
    33601 ggtaatcaac ctggccggag aaccgattgc tgataaacgc tggactcaca agcaaaaaga
    33661 gcgtctctgc caaagccgct ggaatatcac gcaaaaactg gtcgatttga ttaatgctag
    33721 cgacacgccg ccgtcggtac tcatttccgg ctcggcaacg ggctattatg gcgacttagg
    33781 tgaagtggtg gttaccgaag aggaaccgcc gcataacgaa tttactcata aactctgcgc
    33841 ccgctgggaa gagattgcct gccgggcgca aagtgacaaa acgcgagtgt gcctgctgcg
    33901 aaccggcgtg gtactggcac cggacggcgg tattctcggt aaaatgctgc caccgtttcg
    33961 tcttggcctg ggcgggccga ttggttccgg tcggcagtat ctggcctgga ttcatattga
    34021 tgatatggtc aacggcattc tctggctgct ggataacgag ctgcgcgggc catttaatat
    34081 ggtttcgccc tacccggtac gcaatgaaca atttgcccat gcgctcggtc atgcgctgca
    34141 tcgcccggct attttgcgcg tccccacaac cgccattcgg ctgttaatgg gcgaatcttc
    34201 agtactggta ttaggtggac aacgcgcgct gcctaaacgg ctggaagaag cgggttttgc
    34261 gtttcgctgg tacgatttag aagaggcgct ggcggatgtc gttcgctgat gtggtttaca
    34321 gcaaacatcc gccagttaac tccctgtgtt acaggattag tggctttgcg cgataagatc
    34381 gtctggtgaa agtcgggtca ccatcataac taactctctg tctaaacctc tatccagcat
    34441 ctcctgagca atacgcaggg cttcttcgtg tttgccctgc attgcgcctt cttcacgtaa
    34501 tctgtcagca atggtcatca gtttctcctt ttcttgtggt gcgcgttctg ttatctcacc
    34561 aataaatgca cggaaacgct gggcatcccc tgtttgtaat acgtaattaa acagggcttt
    34621 tagctgtctg tcattagtgt tccctgtaac tagcagcgaa acaatttggt cgactaatcc
    34681 caacagatcg cgctgacgaa tatgtttctg aattaactcc aacagcgcca ttttgcggtg
    34741 ttgcataatc tcgtcatccg gcaccacggt aatatccacc aacggaaacg ctgatgaata
    34801 tattttgcgg gctatagcag gctcggcaaa ttcatcaagc cagcagagtg aataaggata
    34861 aggacttctg caaccatgat aaaacagcat cgggatcacc aatggaagct ctttatagcc
    34921 cgcatcaaga tggttttgca ttgccgcaat ggaataacgc atcatgcgaa aagccattaa
    34981 ttcttccggc ttactttggt gctctatcac tacataaata taacccactc cctcctgcgt
    35041 tttcacagac cacaagaggt cggaataata ttgccgcagg tcttcatcaa taaaactgtt
    35101 tggttctagt ttaagcgtcg ttaaatcaca cagtttgcgc agcggcgcgg gaagatgaat
    35161 attaataaaa ttccgcgcgg tgtctggatg gcgtaaaaaa gatttaaata ccgcatcatg
    35221 cggcgtggaa gttgtcgata ttgtcatggc gttccgtcac ccctcaaatt taagatgacg
    35281 cgacaataac cacgattaac acccaatgca gcaggcagtt tttatctttg gacagcgaat
    35341 tcagaagtat ttttagcgga ctgcaacttt tacatttcag cggaagctga ctcgcaaatt
    35401 ccccttgaaa atactccggc agcgttagcc accggagagg aaattatttc agcgatccct
    35461 taaggaactg ttgcagacga gggctttgcg ggttgccaaa taactgctcc ggcgcgccct
    35521 cttcttctat tttcccctga tggaggaaaa tgacgtgagt agaaacatgg cgagcaaagc
    35581 ccatttcgtg agtcactacc accatggttt tcccctcttc tgccagttgc tgcataatac
    35641 gtaacacttc gcctaccagt tcaggatcga gcgccgaggt aggttcatca aacagtaaaa
    35701 cttctggttc catcgccagc gcccgcgcga tagaaacacg ctgttgctga ccgccggaaa
    35761 gatgcaccgg atatttcccc tgcgcacgtt cgtctatccc gactttcgcc agatacttca
    35821 ccgcccgctc gcgcgcttcc tgcttgctca ggcccaacac ctgaatcggc gcttccatga
    35881 cgttttccaa caccgtcata tggctccaga gattgaagtg ctggaatacc atcgtcaggc
    35941 gtgtgcgcag taagcgcagt tgatttttat cggcgacttt gagttgaccg tctttgtcgc
    36001 gcaccagatt gatcgtctgg ccattgacca cgatcgatcc ttcactcggt ttttcgagga
    36061 agttaatgca gcgcagaaag gtacttttcc ccgatcccga cgatccgatg atgcttatta
    36121 catctccggc attcgcttgc agtgagaccc ctttcagcac ttcgtgttcg ccatagcgtt
    36181 tgtgcaaatc gataacgttt aatttattct cggacatcgt attctcagtg cgttgaagaa
    36241 ggtttcacat gctgcaacca gcgtttttcc gctctgcgaa agaggctgat caggacataa
    36301 gagatgatta aatagagcac cgcggcaatg ccgaaggcgg taaaaggttg atacgtggcg
    36361 gcgttaatat cgcgggctat tttcagcaga tccggcacag tggccgtaaa cgccagcgca
    36421 gtagagtgca gcatcaggat cacttcgttg ctgtatgccg gtaacgcaat acgcagcgca
    36481 gaaggcaaaa taatgcagcg atacatttta aaggtcgaga agccataggc tctggcggct
    36541 tcaatttccc catgcggaac cgaacggatt gccccagcaa aaatctcggt agtgtaagcg
    36601 caggtgttaa gcgtcagcgc cagcacggta cagttcaggc cactgcggaa gaaagcgtta
    36661 aggaattcgg ttcccttaac aatctcaagc gtgtacatgc cggaatagaa caccagcaac
    36721 tgaacataca gcggcgtacc gcgaaaaata taggtaaata accagattgg aaactggatg
    36781 tatttattac tggagacacg accaatcgcc agaaacagcg ccaggactcc gcctatcact
    36841 accgacaaaa taagcagcca cagagtgatc gccacaccag taaagcgata accgtcggtc
    36901 cacagcagcg gtttccagta ttcatgtaag atttcgatca caggtcagcc ctcttcacac
    36961 ccacggagta gcggcgctca aggaacagca gcacaccatt ggaaacggtg gtgaaaacca
    37021 ggtaaatcac gccacagacg atggcgaaat agaacggttc ccaggtactt ttgcctgcca
    37081 gctgcgtggc tttgaccaca tcttccaggc cgagtaacga aaccagtgcg gtagatttga
    37141 ggatcacctg ccagttgttg ccaatgcctg gcagggcgta acgcatcatc gccggaaaca
    37201 tgatccgccg aaacacttgc ccgcgagtaa aaccgaacgc cgtcgccgcc tctatatgcc
    37261 ctttcggcac tgccataaac gcgccacgga acgtttcggt aaaataagca ccgtagataa
    37321 aaccgagagt gataatacca gcgaccatcg gatcgatatc aatctgcccg acgcccatcg
    37381 cctccgtcac catgtttagc gcaatctgca aaccatagaa aatcagcagc atcaacacca
    37441 agtccggcac gcctcgaatc agcgtggtgt acccttcgaa aatcagcccc gaaagccgat
    37501 tttgcgagag cttaccgcca gcgccaatta aaccgatgat tacagcgagc actacagagc
    37561 tgatagccag ctccagcgtg acgagcgcac cctgtaaaat aacacctgaa aacccataca
    37621 acatgctgcc tgtcctgtcg tgtgtggtga attaccgcct cttgtctccc tccggttacc
    37681 cgaagggagc gggctttctt tcaccgatgg cgattaacca ccataaacat caaaatcgaa
    37741 gtactttttc gctaatttct cgtaagtacc gtcagcgcgc atttcggcaa aggctttgtt
    37801 cagtgcctcg cgcagttcgt tatcttcttt acgcaggccc atgccggtcc ctacgccaaa
    37861 cagtttttca tctttaacag acggaccacc gaatttgtaa tctttaccga cgggttgttt
    37921 gaggaaacct tcgctggcag cgacctcatc ctggaacgcg gcatcaatac gtccggcagt
    37981 caggtcagaa taaatgttgt cctgcccctg atacgagacg atttcaatgc cttttggtgc
    38041 ccagtgttca ttaccgaacg tctcctgggt ggtgccctgc aatacgccta cccgtttgcc
    38101 tttcagcgac tcgactgtcg gctgaatgtc agaatttttc gccaccacca gacgagaatc
    38161 ggcagcgtac agtttgtcgg tgaaggcaat ttcttgctga cgtttttccg taatggaaag
    38221 cgatgacatg atggcgtcaa tcttcttcgc ttttaaggac gggattaacg catccagcgg
    38281 gttttcgaca aacgtacatt gcgtattgat gcgcttgcac aactccttcg ccagctcgat
    38341 atcgaaacca accagttcgc cttgtgaatt ttttgattca aatggcgcat aggtcgggtc
    38401 ggtaccgatg cggatgtttt gcggaattgc agcaagtgcc gcagttgcgc tggagaaggc
    38461 cagaaccaga gagagcgata gcaccagttt tttcataatg tcctcaactg acagtctttt
    38521 tatagggaat atttacaggt ttgttacact tatcgtgcca taaaattgac catcaaggca
    38581 aaatatcccg ccccgttaga cgtattattt ttcattaaaa atgcttaagt atgtatttca
    38641 atgcaccacg atagtgcaac aaacctggcg tgcaccatga tgatcattcc atcaggtaca
    38701 gcttcccagc gacgtatcag tcaccgtaga cattaaagtc gaaatacttt ttcgccatct
    38761 tgtcgtaggt gccgtcctga cgcagctcgc caagcgcctt attgaaggca gccgtcagtt
    38821 cagcatcatc tttacgtagc cctacaccgg tgccatcacc gaagtatttt ttgtctttta
    38881 ctgatgagcc agcaaaagcg aaatctttac cagcaggttg cttgaggaat ccttcgctgg
    38941 cagcaacttc atcttgtaac gcagcatcca gacgtcctgc agccagatcg gaatagacca
    39001 aatcctggtt ggcataggcc accacatcca cgcctttact acgccaggtc tcgttagcgt
    39061 aagcttcctg ggttgatccc tgcagcacac caacatgttt acctttcagt gaatccagcg
    39121 ttggctgaat cggtgaacct ttggcagcaa tcaaacgaga atctgcggcg tacagcttgt
    39181 cggagaaggc aatctcctgc tgacgtttat cggtaatgga aagcgacgaa ataatagcgt
    39241 cgattttttt cgctttcagt gaggggatca gcgcgtcaaa gtcactggca acccaggtac
    39301 atttcacctg catccgtttg cacatctcgt taccgagatc gatatcaaag ccaacaaaat
    39361 cacctttagc atctttcgac gagaacagcg cataggtggt atcggttccg atacgtaccg
    39421 tctccggtag cgccgcatag ctggaagccg ctgcggagag accgactaat aaagacagag
    39481 cgagaatcga cttcttcata cataaccctc aagtgaaaat ggctttttta tgttgtgtat
    39541 tgtgttgtgt gtttgcaggc tctttcatgc aggtcttatg ccatcttgac gacaacagta
    39601 acattcaacg ttaaatatgt taataagacg ttgcattatt gtcctgaagt tgaagatagc
    39661 aggtatagcg gttgaatcgc agcgtttcga ttgaatggca gaaacaaaat gtcgaggatt
    39721 tgatcgcggt tgcaaaattg ccctgaaaca gggcaacagc ggagttatgc gccctgccag
    39781 cgggcaaaga gatcttcagg aagggttatt gcaaactggt caagaacacg attaaccgtc
    39841 tgatttatca catcatcaag ggattgcggg cgatgataaa acgccggaac gggaggcata
    39901 atcaccgcac cgatttctgc cgcctgagtc attaaacgca gatggcctaa gtgcaatggt
    39961 gtttcacgca cgcagagcac caacgggcga cgctctttca gcaccacatc tgccgcacgg
    40021 gtcagtaagc catcagtata gctatggaca atgccggaca gggttttgat tgaacagggt
    40081 aaaatcacca tccccagcgt ctggaaagaa ccggaagaga tgctggcgga aatatcgcgc
    40141 gcatcgagcg tgacatcagc taatgcctgc acttcgcgca gggaaaaatc cgtttcgagg
    40201 gataaggtct ggcgcgccgc ctggctcatc accagatgcg tttcgatatc tgtgacatcg
    40261 cgcagaacct gtaataagcg cacgccataa atcgcgccgc tggcaccgct gatgcctaca
    40321 atgagtcgtt tcataaatag ttgccctgtc agacttgcag gcagactttg cagtatttcg
    40381 gcgggagttg caagtcaggg tgccagaccg gcaccctcag cgaaggcatc atccttcgtt
    40441 atgcatttcg agattttcca cttcgttctg acgttgcact gctttggcgt catcattacg
    40501 taacgtatcg aggaaatcga ggtagccctg atcaacatct ttggtgacgt agacgccgtt
    40561 gaacaccgag cattcaaact gctggatatc cggattttca gcgcgaacgg cgtcgatcag
    40621 atcgttcaga tcctggaaaa tcaacccgtc agcaccgatg atctggcgaa tttcatcaac
    40681 ttcgcgaccg tgagcgatca gttccgtggc gctcggcata tcaataccat aaacgttcgg
    40741 gaagcgaatt tccggtgccg cagaagcgag gtacactttc ttcgctccgg cttcgcgtgc
    40801 catctcgata atctgctcag aagtggtgcc acggacgatg gagtcgtcga ccagcaggac
    40861 gtttttatcg cggaactcgg cgcggttggc attcagttta cggcgcacgg acttacgacg
    40921 cagctgctgg cccggcatga taaaggtgcg gccaacatag cggtttttaa cgaagccctg
    40981 gcggtacggt ttgcccagaa tacgagcaat ttccagcgcg atatcacacg aggtttctgg
    41041 gatcgggatc accacgtcga tatccagatc ttcccattcg cgggcaattt tctcgcccag
    41101 tttcgtgccc atattcacac gcgcgctgta aacggaaatt ttgtcgataa acgagtccgg
    41161 gcgggcaaag tatacatact caaacaggca cggattgctg accggattgt cagcacattg
    41221 acgggtaaac aactgccctt cttcagtgat gtaaatcgct tcgcccggcg cgacgtcacg
    41281 caggaaatca aagcccagcg tatcgagcgc tacgctttcg gaagcgacca tatattctgt
    41341 acggttctcg tcaatatcac gttttcccag taccagcgga cgaatcccgt ttggatcgcg
    41401 gaaagcaacc ataccgtggc cgataatcat cgccacacag gcatacgcgc cgcggattaa
    41461 gcggtttgtg gcagcaatgg cagcgaaaat attgtcggct tccagcgggt agtggcggaa
    41521 gttgtccagc tcgctggcga agatattaag cagaatttcc gagtcggaag tggtgttgat
    41581 gtggcggcgt ttttcttcaa acagtttttt acgcaactcg tgagcgttgg tcagattgcc
    41641 gttgtgggca agcgtaatgc catacgggga gttaacgtaa aacggctgcg cttcagaggc
    41701 gctggagctg ccagccgtag ggtaacgcac atgaccaatg cccatattgc cctgcaaacg
    41761 ctgcatatgg cgagcttcaa atacatcgct caccagcccg ttcgctttac gcaaacggaa
    41821 gcagttattg gcatctatgg tgatgatgcc ggcggcatcc tgaccgcgat gctgaagcac
    41881 cgttaaggca tcataaatcg actggttaac cggcataaca ccggcgatac cgacaatacc
    41941 gcacatacgt ctttttcctc gttaagccac atctcagagc acttacgctc tgggcaagaa
    42001 acttgacgag ctttgcagat aatcaaaaaa ccatctgatg ataaaactga attgcgggat
    42061 cagctgtgat ttgctccagt cttcactttt cgacaccccg gtaaaggagt cgagaaagaa
    42121 gagaatggcg gcaacaatca acacaccgcg caacgcaccg aaacagacgc ccagcacccg
    42181 atcggtgcct gacaaccccg ttttctccac caactggcct atcacgaagt tcacgatagc
    42241 accaacgatc agggtagcga taaacagtac cgcgatggca atcccatttc gaaccagttc
    42301 gtcttcaaag cccgtaaacc agactgacag gtaagtgtag taatgactgg caacaaagaa
    42361 agcacaaccc catgtcacca gcgataacgc ttcacgaaca aagccgtgga tcaggctaac
    42421 cagagaggaa aaagcaatca ccgcgattat ggcgtaatca atccagacca tatgtgtccc
    42481 acgattttac gccctgtcat cctgttcggg gcgcattcta acagaaaaag aaaacgtttg
    42541 cgtagggatt tccttcccgc gcatcaataa aaatggcgct gaaaaaatat tcaacgccat
    42601 cgacttttta tgcctttgcg gcatcgtgca atgcgtgtcg gatgcggcgt aaacgcctta
    42661 tccgacctac ggttctaaca ctgcccaagc ctgataagac gcgccagcgt cgcatcaggc
    42721 aagactgtat taattcggcg tatagcccat taccacgcca cttaagccag aaagttgctt
    42781 caactcaccc agcgaacctt tcagcttatc tttcgaggca tccggcccaa ccagaatacg
    42841 ggtaatttta ccctgcactg gcgtggatgg cgacgtataa acccgataac cggcaccgcg
    42901 cagcttaccg acaatctcat tcactttatc ggcatttttc agcgcaccca gttgcacaac
    42961 ataggcttta cccgttggcg ctgctttttc ttccacgacc ggcttcggtt ctggcttcgg
    43021 cgcaggtggt gcttcaacct ttggtttagg cggctccacc ggtttcggct tcggtggggc
    43081 gaccggtgca ggttccggtt caaactcggt gttattggct gcaatagtgg ccggatcgag
    43141 cgacggtgct gcggcatcac ctgcccgcac ctcttccgct gcgccttccg gcggctgcgt
    43201 cggcaacgcc tgggtggcgg ctggcatcat atcaggctca tcacgatcgc ccgctttcgg
    43261 caccagcggg atagccgcga actcatcctg ataatgtttt ttctgcccgt ccagcagccc
    43321 tggaagtaca atcaccccca gcgccaccag cacgatcgtg cccactaacc gattctgaaa
    43381 cttacttgcc accgcttctc ctcgcgtcaa tcacttccat gacatgtgcg accgtgtgga
    43441 aagaaccaca caccagcacg gtgtcttccg ctttagcgtc cgccattgcg gcatcccatg
    43501 cctgcgcaac gctatcaaat gatttgccgt tacccaaatg ctcaagcagt tgttctgccg
    43561 tggcaccgcg cggcccttcc agtggcgcac aataccagtc atcaaccacg cttttcaacc
    43621 aggccagagt tccggcaata tctttatcat gtagcatacc gataaccgcc agcacgcgcc
    43681 cgtttttcgg tagcgctttc atacgcccgg tgagatattc cgccgcatgt ggattatgcg
    43741 cgacatcaaa aataacgcgt ggcgactcgc tcacaatctg gaaacgtccc ggcaaaattg
    43801 cgctggcaat cccgtcgcga atggcatttt cactgacttc cagcccgctg gcacgcagtg
    43861 ccgccagcgc cgttgcggca ttcggttgcg ggacaagcgg caacggcaga ttttccagcg
    43921 tgccgtgagc atcgctaaac gcccagtcat gatcggtgac ggaatagttc cactcaacgc
    43981 cccgacgttg taacagtgca cctttttcct gcgccacatc agcaatggta gaaggcattt
    44041 ccggctcacc gacaattgcc ggtttttcgc tgcggaagat gcctgctttc tcgcgaccaa
    44101 tactttcgcg atctggaccc agccagtcgg tatgatccag cgcaatactg gttactaccg
    44161 cgacatcggc atcgacaata ttggttgcgt ccagacgacc gcccagccct acttccagaa
    44221 tcaccacgtc aagttgtgcc tgcttgaaca gccacaacgc cgacagcgta ccgtactcga
    44281 aataggtcag ggaaatatca ccgcgtgccg attcaatctc cgcaaaagag gcggtgtggg
    44341 ccgattccgg caattcctgg ccctgcacac gtacgcgctc ggtataacgc accagatgag
    44401 gcgaactgta gacgcccact ttgtaccctg ccgccatcag aatcgactcc agcgtacggc
    44461 aggtggtgcc tttgccattc gtacccgcaa cggtaaacac aaatggcgct ggtttcagga
    44521 cgccaagacg cgccgcgacc tggctcacgc gctcaaggcc gagatcgata gttttactgt
    44581 gcaggttttc cagataagaa agccacgaag ccagaggcga cgcggcttga ggagtgcgtt
    44641 tgataatcat ggtatccgct gattcgttac ggtgagaata gcaaaagggc agagccagtg
    44701 gccctgccct tatcagttat caggcctcag gttcctgatc cggtaccggg ggtaccacta
    44761 cgccttcacg cggcgcttca ggattcggcg ctggcagatt catcaacttc gccagaatgc
    44821 tcgccagttt caggcgcatt tccggacgac ggacgatcat gtcgatcgcg cctttctcga
    44881 tcaggaattc actgcgctgg aatctaggcg gcagtttttc gcgaacggtc tgttcgataa
    44941 cacgcggacc ggcaaagccg attaacgctt taggttcagc gatgttgaga tcgcccagca
    45001 tggcgaagct tgcagaaaca ccgcccatcg tcgggtcggt cagcacggag atgtacggca
    45061 agccgcgctc ctgcattttt gccagtgccg cagaggtttt cgccatctgc atcagcgaca
    45121 tcagtgcttc ctgcatacgt gcgccaccag aggcggagaa gcagatcagc gggcagttat
    45181 cttcctgcgc ctgctcaacg gcacgcacga aacgtgcacc cacaacagac cccattgaac
    45241 cgcccataaa ggcgaactcg aatgccgcag cgacaaccgg cattccatac agagtgcctt
    45301 tcatcaccac cagcgcatct ttttcgccgg tttctttctg cgcagatgcc agacggtctt
    45361 tatacttctt ggagtcacga aacttcagct catctttcgg ctcaagctcg ctacccagct
    45421 ccacaaggct tccttcatct aacaggctat gcaggcgatt acgcgctgtc atacgcatgt
    45481 gatggtcaca cttcggacag acctcaagat tacgttccag ctcagcgcgg tataaaacct
    45541 gaccgcagct atcacactta gtccacaccc cttcaggaat gctcgccttg cgggtgggag
    45601 taatgttgct tttaattcgt tcaatccagc tcattaggga cctttctgtc tgaacctggt
    45661 tcgatgccag ttttatcttt ggggacgcat aatgccattt ttgcccccaa cagaccatga
    45721 atgttgcaca ttaaaacata acagcccgaa actttggata aaaaagtggt cgaaccgcgg
    45781 agttactttt tattttgcgg cgcgtgccgc agcgcgtttg tgacggatta tttcgatgac
    45841 gcccggcaaa atagaaacca caataatccc gacgatcagc agcttaaggt tatcctgaac
    45901 catcgggatt gtaccgaaga aatagcctgc gtaggtaaaa agcagtaccc acaacagtgc
    45961 gccgatcacg ttataagcag cgaaatgacg gtacgacatg tggcccattc ccgcaacaaa
    46021 cggggcgaac gttctgacga tcggcacaaa acgggcgaga ataatcgttt tgccgccatg
    46081 tttctcataa aactgatggg ttttgtcgag ataactgcga cggaaaattt tcgaattggg
    46141 attactgaat aacttttcac cgaacaaccg cccgatagtg taattgaccg cgtcacccac
    46201 aatcgcggcg atcagcatca gcactaccat catatggacg ttgagatcgt tggtttccag
    46261 tgatgccaac gctccggcaa caaacagcaa tgaatcaccc ggtaaaaacg gcattaccac
    46321 cagaccggtt tcacagaaca aaattaagaa caaaatggca taaacccaga cgccgtactc
    46381 cgcgactagc tctgccagat gcacgtcaat gtgcaggata aaatcgatga ggaaataaat
    46441 caggtccata tttgcctatg ccttgtactc gtcatctttc aggctgtaac tacgttggct
    46501 acagcctgaa atgttccgag cattattctc gttagtccgc cagaaatagc gggcccattg
    46561 gcggttttgg aagatcatac cggtcagggt aatccaccgc gaccagatac agcccttccg
    46621 cttttgccgt tgctgccgcc agcgttctgt cctttgccgc cagcaactct gctatccagc
    46681 tctccggctg gttgtgggca ccgacttcca tcaggctgcc gacaatattc ctgaccatat
    46741 gatgtacaaa ggcattcgct ttgatatcaa ccaccacata aggaccgtga cgcgtgacat
    46801 taatatgcat aacgttgcgc cacggggttc gggactggca ctgcaccgca cggaacgagg
    46861 tgaaatcatt ctcgcccagc aagcattgcg cagcccgatg catccgttca gcgtccagcg
    46921 gttcgtaaaa atgggttacc cctttactca gtaccgccgg gcgcagccga tgattgtaga
    46981 tgatgtagcg ataacggcga gccgtggcgc taaatcgtgc atgaaaatca tcaggtacag
    47041 ctttaaccca acgcacggcg atgtcaccag gtaaattcgc atttacgccc aaggtccacg
    47101 ccgcgtcttt gcgctgcgcg gttgtttcga aatgcaccac ttgcccggta ccgtgtaccc
    47161 ctgcgtcagt acgcccggcg cagaagacgg tgatgggttc gttcgccacc tgggagagcg
    47221 ccttttccag cttctcctgc acactgcgga cttcattctg ccgttgccag ccgtaatact
    47281 tactgccgtc gtactcaatg cccagcgcaa ttttataaac tggcagttgt tgctggtcgg
    47341 acattagtac agatactcct gcaccagttt ctcggcgatt tttactgcca tcagcgcgcc
    47401 gccaaagcga acgttatcgg ccaccgacca gaactggact tgctccggca taccgtagtc
    47461 attacgcacg cagccaacag aaagatgcgg cgtacccgaa gcatccccta cctgagtcgg
    47521 gaattcgttc tcttcagaga gcacaatatc ttcgccctga gcaaacgcat cacgcgcttc
    47581 ttctgccgcc agcggacgca gtgcttcaaa gttgaccatc tgggcatgac cgtagaatac
    47641 cggtgcctgg acgacgcttg ccgaaatcat cagcccttcg tcctgcagga ttttgcgtac
    47701 ttcgtcaacg atacgacgtt cttcacgcac gctaccttcg ctatccggca acaacggcag
    47761 catattgaac gccagttgac gaccgaagaa atcttcttcg tcaatcggaa tgccgttgag
    47821 caatttcgca ctctgccccg ctaacgcatc gaccgctttt ttgccctggg cggaggctga
    47881 aatcaggctg gtaacactga tacgcgacaa accgccctga tcgattaacg gtttcagtgc
    47941 cgccagcagc tggctggtca gactgtctgg tacggcgatg acattccggt tgcggtaatc
    48001 ggtcagtaca aacgggttta cttccggcac caccagcggt acgtcgggtt cgagagcaaa
    48061 cagtccgctg ctgtcgatca ccaggcaacc tgagttggtc gcttcttcaa cccaggcagc
    48121 ggtagcttct ttgcctgcga caaaaaatgc cagctgcgcc tgcgtccagt cgaattcagc
    48181 ggcatcctgc acggtgattg ttttaccacc aaagcgcagt tgttcgcctg cgctttcgtt
    48241 acgtgccagt gcataaattt ccccaaccgg gaactgacgt tcagccagcg tttcaagcag
    48301 ggcttcgccc atagcgccag ttgcgcccag gacggcaatg ttccagcctt cagacatggt
    48361 ggtttactcc agaaatagca aagctccctg ccaatgttac agcagagagc atgaagaaga
    48421 gattaacgtg ccggatgatg aacggcgtta aaacccagtt tacacagcaa tgatgccgca
    48481 ctggcgtcat cacaaattac atacagagat gaccattcac ggcgctcaag atagtttttg
    48541 cgcagtttat cgaactcacc cggtatcccg gcgactttac gcagcggtgc gtcatcgcgg
    48601 cgcacatcat acaccaaatg caccagcctt ttcagcgtcg gttgatcgag cggaccatgc
    48661 agcgtaatgc ggccaaactc aggcgcgggc agcaacgtat ccagcgcaac gtgttgttca
    48721 tgcccaataa acttgctata ggcttcaaac acttgcgtcg tgccacgcgc tttaccctcc
    48781 agggtatagc ctgcgatatg cggcgtgccg atatccactt ttgtcagcaa ttccacatta
    48841 agttccggct cgccttccca gacatccagc accacgctta acttctggcc ttcattcagg
    48901 caggtcagca gcgcggtatt atcgacgact gcgccacggc aggcgttaat cagaatcgct
    48961 ccgggcttca ggctacggat cagtttttca tccgccagat gtagcgtttt gtacggacca
    49021 tctttaaaga gtggcgtatg gaaagtcaga atatccgcgc gctggactaa ctcatccagc
    49081 gagcggaaat caccctcatc cccacggtcg gcgcgaggcg gatcgcaaag taaggtttta
    49141 atccctaacg cttccagtcg cgcctgcaat cgacgtccaa cgttaccaac gcccacgatc
    49201 cccaccgtac ggtcgtgcag tgaaaatcca tcgcgttcgg caagcatcag cagggaggag
    49261 aaaacatatt ccaccaccgc aatcgcatta cagccaggtg cagcggaaaa accaattccc
    49321 gcctgcttca accatgcttc atcgacatgg tcggtccccg cagtggcagt tccaacaaat
    49381 ttaatgggtt ttcctgccag caaagattca ttcactttcg tgaccgaacg caccatcagc
    49441 gcatccgcgt ctgccagttg agcgacgggg attggacgcc cgggaaccgc gatcacctca
    49501 cccaaacggc taaataagtc gcgggcataa ggcatatttt catcaacaag gattttcacg
    49561 tttgtgttac ctgtatgaga cgagagttaa ccggacaagt atgccataat ctcgcggcca
    49621 ggcatacttg cgaagatttc aggtataagg atacgtaatg atacaaccta tttccggccc
    49681 tcctcctggg cagccaccag gtcagggaga taacctgccg tctggcgcgg gcaatcagcc
    49741 tttatccagt cagcaacgta cttcgctgga aagcttaatg acgaaagtga cctcactgac
    49801 gcaacaacaa agagcagaac tgtgggcggg tatcaggcac gatattggtc tgtcgggaga
    49861 ttcaccgctg ctttcgcgtc acttccctgc cgctgagcat aatctggcgc aacgtctgct
    49921 ggccgcgcaa aaaagccatt ctgcccgcca gcttttagcg caattagggg agtatttacg
    49981 tctggggaat catcgtcagg cggtcacgga ttatatccgt cataactttg gtcagacgcc
    50041 gctgaatcag ctctcaccgg agcaattaaa aaccattctc accctgttgc aggaagggaa
    50101 gatggttatt ccgcaaccac agcagcgcga ggcgaccgac cgtcctttat taccggcgga
    50161 gcacaatgcg ctaaaacagc tggtgaccaa acttgcggcg gcaacggggg aacccagcaa
    50221 acagatctgg caatcgatgc tggaactttc cggggtgaaa gatggcgagt taattccagc
    50281 gaaactgttt aaccatctgg tgacctggct acaggcgcgt cagacgctaa gccagcaaaa
    50341 tacgccgacg ctggaatcac tacagatggc gctaaaacaa cctttagatg ccagtgaact
    50401 ggcggcgtta tcggcatata tccagcaaaa atatggcctt tctgcgcaat catcgctttc
    50461 ttctgcccag gccgaggata ttcttaatca gctttatcaa cggcgggtta aagggattga
    50521 tccgcgtgat atgcaaccgc tgcttaatcc ttttccaccg atgatggaca cgttgcaaaa
    50581 tatggcaacg cgtcccgcgc tgtggatact gttagtcgcg attatcctga tgctggtctg
    50641 gctggttcgt taaccccgac gaaatgacag tatcgtgaca ataataccca gcaccgcaga
    50701 gatcgccccg gcaagaaata ccgaagagta accaaacgtg gtcgccagca ttcccgccag
    50761 cggcccggag acgccgaggg cgatatcctg aaacgcggcg taaccgccca gtgcggtgcc
    50821 gcgaacttgt gaggggacgc gtttaaccac ctccacgccc agcgcaggaa agataagcga
    50881 acatccggct ccggttaacg ccgcgcccgc taatgcgacc catgcacctg gggcttgcca
    50941 gagcagcaac aagcccaccg tttctacaag cagagagaca atcgccactt tcacgccgcc
    51001 aaaacggtcc ggcatccagc caaacatgac gcgcatcacg acaaatgcgc cgccaaacgc
    51061 ggtaagagta aagcccgcca tcgcccatcc tttgctggca aagtagagcg aaacgaaagt
    51121 cccgataacc gcaaaaccaa cgccttgtag tgccagccct aaccctggtt tccagataag
    51181 cccgacaacg ctccacagcg atggacgttc tcccgccagg gccggtactt tgcgcactgt
    51241 gccgttacag gcccacgcca gtaagggtaa taccattgtg gtgatcgcca gtgcggcaaa
    51301 accgtaatgg ctatgaatca acaggccaag cggcgcacca acagcgaggg caccgtaaat
    51361 cgccattccg ttccatgaca tcactttgcc agagtgtttt ggccctacga tgcctaaccc
    51421 ccaggtcaga gcgcctgtca gtaactggct ttcaccaaac ccaagaatca aacgcccgat
    51481 gaccaacagg gcaaatttga acggtgcgga gacaggcaaa atcgccgcca gcagcaacgc
    51541 gccgccagcc agaccacaag ctaacatccc ctgaagcgcc gaacgttttg cgccatattg
    51601 atcggccagt cgcccggcgt aaccacgcgt cagcaccgta gccagaaact gaatcccgac
    51661 ggcaatgccg accatggtat tgccatagcc cagatcatga tgaacaaaca gcgggataac
    51721 cggcaacggc aaccctacgg tcatgtaggt gagaaaaacc gcaaaagaga tgcggaagag
    51781 cgaaaaactg gcagaagatc gtgtttcggt ttggcttaca gcagtcatgc attactccag
    51841 aatgcagcgc aaggcgagga gtatccccgt ctcatctctc tggtttcagg gttacggtgc
    51901 gttggcagga tttaacgcgt acgtcttttc agaaggaaat cgacaaagcg ggaagtttgc
    51961 ctagaactgg tggcgattgt caatgatgtg aaaaagggaa ccatcaggtt cccttttgcg
    52021 ttagtgccgg aggccgcatt ggcgcgtaac gtcggatgcg acgctggcgc gtcttatccg
    52081 acctactccg gcctactgcg aattaatctt ttcagcttgc gcattaccag cgtggcgttg
    52141 gtgccgccga agccgaagct gttagacata acagtggtca gttcgcgatc ggtcgtttcg
    52201 gtcacgatgt tcagacccgc agcctgctcg tccagctctt caatgttgat gctcggggcg
    52261 ataaagccgt gttccagcat cagcagagag tagatagctt cctgcacacc cgcagcgccc
    52321 agtgagtgac cggtcatggc tttggttgca gaaatcgccg ggctcttatc gccgaacact
    52381 tcacggatag ctgccagctc tttcacgtcg ccaaccggag tcgaagtacc gtgagagttc
    52441 aggtaatcga ttggggtatc aacgccatgc atcgccatct gcatacaacg tactgcgcct
    52501 tcgccagacg gagcaaccat gtctgcacca tcagaggttg cgccgtagcc aacgatttca
    52561 gcatagatgt aagcaccacg cgccagcgcg tgttccagct cttcaaccac taccataccg
    52621 ccgccgccag cgataacgaa accgtcacgg tgagcgtcgt aagtacggga ggctttttcc
    52681 ggggtgtcgt tgtatttagt agatagcgca cccatcgcgt cgaattcgca ggccatttcc
    52741 cagcacagct cttcgccgcc gccagcaaac acgatgtcct gtttgcccag ttggatctgc
    52801 tctactgcgt taccgataca gtgtgcggaa gtcgcacacg cggagctgat ggagtagtta
    52861 acgccatgaa ttttaaacgg cgtggcgaga caggcagaaa cgccggatgc catcgcttta
    52921 gtgaccacat acgggccaac cgctttcagg ccgcgcgggc cgcgcattgc gtcagcgccg
    52981 aacacctgga aacgcgggga accgccgccg gaacctgcaa tcaggccaac gcgcgggtta
    53041 ttctggtaag cttccggaga gaggcccgca tcagcgattg cctgctccat agaaaggaat
    53101 gcataaatgg atgcgtcgct cataaagcgc acaactttgc ggtcaatgag gccagtggta
    53161 tccagtttta tgttgcccca gacgtggcta cgcatgccgg aatccttcag ctcctgagag
    53221 aaagtgatcc ctgaacgtcc ttcacgcaga gatgccagga cttcctgctg gttattaccg
    53281 atgctggaaa caatgcccag gccagtaatc actgcacgtt tcattcaata cctctgtaag
    53341 tcgcacatag agtaagtttc gaatgcacaa tagcgtacac ttgtacgccg aacaagtccg
    53401 atcagccatt taatagagat atttgcgcag ccttacacac atcgctaaga tcgagccacc
    53461 gcctgtaaga cgagtaactt acgtgaaaca ctactccata caacctgcca acctcgaatt
    53521 taatgctgag ggtacacctg tttcccgaga ttttgacgat gtctattttt ccaacgataa
    53581 cgggctggaa gagacgcgtt atgtttttct gggaggtaac caattagagg tacgctttcc
    53641 tgagcatcca catcctctgt ttgtgatagc agagagcggc ttcggcaccg gattaaactt
    53701 cctgacgcta tggaaggcat ttgatcagtt tcgcgaagcg catccgcaag cgcaattaca
    53761 acgcttacat ttcattagtt ttgagaaatt tcccctcacc cgtgcggatt tagccttagc
    53821 gcatcaacac tggccggaac tggctccgtg ggcagaacaa cttcaggcgc agtggccaat
    53881 gcccttgccc ggttgccatc gtttattgct cgatgaaggc cgcgtgacgc tggatttatg
    53941 gtttggcgat attaacgaac tgaccagcca actggacgat tcgctaaatc aaaaagtaga
    54001 tgcctggttt ctggacggct ttgcgccagc taaaaacccg gatatgtgga cgcaaaatct
    54061 gtttaacgcc atggcaaggt tggcgcgtcc aggcggcacg ctggcgacat ttacgtctgc
    54121 cggttttgtc cgccgcggtt tgcaggaagc cggattcacg atgcaaaaac gtaagggctt
    54181 tgggcgcaaa cgggaaatgc tttgtggggt gatggaacag acattaccac tcccctgctc
    54241 cacgccgtgg tttaatcgta cgggcagcag caaacgggaa gtagcgatta tcggcggtgg
    54301 tattgccagc gcgttgttgt cgctggcgtt attacggcgc ggctggcagg tagcgcttta
    54361 ttgcgcggat gaagcccccg cgctgggtgc ttccggcaat cgccaggggg cgctgtatcc
    54421 gttattaagc aaacacgatg aggcgctaaa ccgctttttc tctaatgcgt ttacttttgc
    54481 tcgtcggttt tacgaccaat tacccgttaa atttgatcat gactggtgcg gcgtcacgca
    54541 gttaggctgg gatgagaaaa gccagcataa aatcgcacag atgttgtcaa tggatttacc
    54601 cgcagaactg gctgtagccg ttgaggcaaa tgcggttgaa caaattacgg gcgttgcgac
    54661 aaattgcagc ggcattactt atccgcaagg tggttggctg tgcccagcag aactgacccg
    54721 taatgtgctg gaactggcgc aacagcaggg tttgcagatt tattatcaat atcagttaca
    54781 gaatttatcc cgtaaggatg actgttggtt gttgaatttt gcaggagatc agcaagcaac
    54841 acacagcgta gtggtactgg cgaacgggca tcaaatcagc cgtttcagcc aaacgtcgac
    54901 tctcccggtg tattcggttg ccgggcaggt cagccatatt ccgacaacgc cggaactggc
    54961 agagctgaag caggtgctgt gctatgacgg ttatctcacg ccacaaaatc cggcgaatca
    55021 acatcattgt attggtgcca gttatcatcg cggcagcgaa gatacggcgt acagtgagga
    55081 cgatcagcag cagaatcgcc agcggttgat tgattgtttc ccgcaggcac agtgggcaaa
    55141 agaggttgat gtcagtgata aagaggcgcg ctgcggtgtg cgttgtgcca cccgcgatca
    55201 tctgccaatg gtaggcaatg ttcccgatta tgaggcaaca ctcgtggaat atgcgtcgtt
    55261 ggcggagcaa aaagatgagg cggtaagcgc gccggttttt gacgatctct ttatgtttgc
    55321 ggctttaggt tctcgcggtt tgtgttctgc cccgctgtgt gccgagattc tggcggcgca
    55381 gatgagcgac gaaccgattc cgatggatgc cagtacgctg gcggcgttaa acccgaatcg
    55441 gttatgggtg cggaaattgt tgaagggtaa agcggttaag gcggggtaat ctgctctcgc
    55501 agtgtttgcc ggatgcggcg tgaacgcctt atccaaccta cgtgtgacgt gtaggcctga
    55561 taagacgcga taagcgtcgc atcaggcatg gtgctccaga cgccgcaact tacagctgtg
    55621 acgcctgctg aaacaggttt tcccacatat cggtcaccag cgcctggtca cgcggcgaca
    55681 attcaccggc accaatggct ttttccagac tctggctaac ggtcgtatgc accgcctgag
    55741 cggagtggtc atcaccactt tccagttctg cgatggctaa tgtcaggtgg ccacgcaaat
    55801 acccactggc aaacaactca tcatcactgg catggtcaac catgccgtcg attaatgcca
    55861 gaatgcgtga ttcaaactcc gcgatcatct tctttcctca ttgtaaaacg tggcgctgac
    55921 ttcagttgag cgcttccggc cacgggaact gttccgccgt aagttccggc gtgtgataat
    55981 aattttgtag tgctttaatg aagcgtgccg ggcgttccgg aatgccattt gtcagataat
    56041 ccatcacctg cgcatgtacc cgccgttgaa acaccacacg atccggttcg aaatcccctt
    56101 ccagattgtc gcaactgacg ttaaacggat atccagccgc cacgcagaat aaccagtcga
    56161 acgcctgcgg cttcacttca acatcttcaa actggctttg cgtctgggca tcgcgtccgt
    56221 ccgggcaata ccagtagccg aaatcaacca gctcacggcg cgcttttccg gcaatacacc
    56281 agtgcgaaat ctcatgaatt gcgctggcat aaaagccatg agcaaagacg attcggttat
    56341 acggtacttc cgcatcagca ggaagataga tcggttcgtc gtcgccttta atcagacggg
    56401 tattaaattc atcggcaaag cagctattaa aaatttcaat caactgctcg tagtggtgtg
    56461 tactgttcat tagttcatcc ccaaccagtg gaggatctcc tgtccgtggc tatcgtaaag
    56521 taatttggca ctcatcaccg ccgagacgat aacaatcatc gggcggatca gcttttgtcc
    56581 tttgctcaac accaaccgtg aacccatgcg cgcgccgagg aactgcccca ccagcatgac
    56641 aaagcccgtc gcccaaatca ctttgccgcc gagaataaac agcagcagac cgccaatgtt
    56701 tgacgttgcg ttgagtaatt tggcgtgagc cgtggctttg gcgaggttga atccgcacag
    56761 cataacgaag gccagcgcgt aaaacgatcc ggcagccggg ccaaagaatc cgtcataaaa
    56821 accgacgcag ccaccggcaa ttaacgcgaa gggtaaaccg tacatccggc gctggcgatc
    56881 ttcttcaccc agctttggca tcagcaagaa atagaggccg atacaaatca ccagaatggg
    56941 caaaatctgc cgtaagacat cagcctgaac gtactgtacc aacagtgcgc cgctcattga
    57001 gccgacaaag gtcatggcaa tattgagttt ctgatcgctt aaactaacca ctttgcggcg
    57061 aataaagtag atagtggcgg aaatagagcc gccgcaggct tgcagtttat tggttgccag
    57121 cgcgttagcg ggagacattc ccgcagccat caatgccgga atggtgagta acccaccgcc
    57181 accggcaatc gagtcgataa atcccgccag catggcgaca aaaaagagaa ctcccagcaa
    57241 cagcggagaa accataaaca ggctattaaa cgtttccatt agatcacgtg ctcatccagt
    57301 agcgcctggc aggaaggcgg caacggaggc ggtgtcttct tctcaggctt tgttgttccc
    57361 ggttttggag gttcaaacca gctttgcagt tctgccccgc aaccatcgcc tgatggcggt
    57421 aaaggttgat cttcacactc cagactatcg gcagggcaac gtaatcgtac atgcatatgc
    57481 gcgcgatgct ggaaccaggg tcgcactttg cgcaaccagt cgcgatcggt gcccgcatca
    57541 aggcaaagtt gttgtttaat cgccggatta acaaaaatgc gcgtgacgtc tttgtcctgg
    57601 gcggcgagtt tgatcaagct gaaaatttct ggcttccaca gcgtggagac aacgtgttta
    57661 ccgtcgcggg aaactaagtc cagtgcttgc gggcgcaaga gctgcgcgga ggtccagcga
    57721 gttttcggca gttgcagaaa gatatcgaca tccagtccgg tctggtggct ggcatgaccg
    57781 ccgttgaaac gcccaccagc gggcatcccc atatcgccaa tcagcaccgt acccatgccc
    57841 agattgctca cctggctact cagacgctgg ataaacatca ccagatccgg gtgaccgaaa
    57901 tagcgacgct gatcggtacg catgacctga taatgttcgg actgtatcgg caacgtgtca
    57961 gcgccgacaa tacagccatt agaaaaactg cctatcgatt gtgcgctacc cggcacaggt
    58021 tgggttattt tttgccacgg cgtcgctgcc aggctagcgc tactggcaag cagagccagc
    58081 agcgcaatcg cggttttgtt cattttttac cagcgtggaa tatcagtctt cacatcggca
    58141 ttttgcgccc gctgccgtaa caggtgatcc attaaaacga tcgccagcat cgcttcagcg
    58201 atcggcactg cacggatccc gacacaggga tcgtgacggc ctttggtgat catctcaact
    58261 tcttcgccaa agcggttaat agtgcgcccc ggcacggtaa tgctggaggt cggtttcagc
    58321 gccatatgag caatgatttg ctgcccgctg ctgataccgc cgaaaatgcc gcccgcatgg
    58381 ttgctctgga aaccgtcttt ggtgatttcg tcgcggttct ggctgccgcg cagcgccact
    58441 acgtcaaaac cgtcgccaat ttccacgcct ttcaccgcgt tgatgctcat cagcgcatgg
    58501 gcgatgtcgg catccaggcg gtcaaagacc ggctcgccaa gtccggcggg gacaccactg
    58561 gcaacaacgg tgactttcgc gccgatggag tcgccctctt ttttcagcgc gcgcatcagt
    58621 tcatctaacg cgtcgatttt gtcggggtcc gggcagaaga acggattttg ttcaaccagc
    58681 gaccagtctt tgatttccag cggaatgtcg cccatctggg tcaggcagcc gcggatttca
    58741 ataccaaatt tctcggcgag atattttttg gcaatcgccc ctgccgccac gcgcatggcg
    58801 gtttcgcggg cggaagaacg tccaccgccg cgataatcgc gcagaccgta tttttgttcg
    58861 taggtgtaat cagcatggcc tggacggaag acgtctttaa ttgcgctgta atcctgagaa
    58921 cgctggtcgg tattttcgat caacaagcca atgctggtgc cagtagtaac gccttcaaaa
    58981 acaccggaga gtattttgac ctgatccggc tcgcggcgct gggtggtata gcgcgatgtc
    59041 ccagggcgac gacgatcaag gtcatgttgc aggtccgctt ccgtcagcgg aatgcctggc
    59101 ggaacaccat cgacgatgca gccgagcgcc agcccgtgcg attcgccgaa ggtggttacg
    59161 cgaaagagtt gtccaattgt gtttccagcc atcacggctc cgttattgtt gtgtttgcgt
    59221 gtttacttaa tctttataaa tcgcgaaatg ttctcgtgcg gcaataagct gctctttagt
    59281 gagcataaac acaccatcgc cgccgttatc aaactccagc caggtgaacg gaacatcggg
    59341 atattgttcc ataagatgta ccatgctgtt gcccacttca caaatcaaca cgccatcatc
    59401 agcaaggtaa tctgccgcgt taccgagaat gcgacgcgtc agtttcaggc cgtcggtgcc
    59461 agatgccagg cccagttccg gctcgtgacg gtattcgttt ggcaggtcgg acatatcttc
    59521 cgcgtcgaca tacggcgggt tagtgacaat cagatcgtat tgcactttcg gcaaatcacg
    59581 gaacagatcg gaacgaatcg gaatgacgtt gtggatcaga ccgtgttctt cgatgttctg
    59641 ttcggcaacc gccagcgcgt cgggagagat atctaccgcg tcgacttccg cttccgggaa
    59701 ggcataagca caggcaatgg cgatgcagcc gctaccggta cacatatcta aaatatgctg
    59761 cggttgcttg ctgatgagtc cggcaaattt attgttgatc agttcaccaa tcggcgagcg
    59821 cggcaccagc acgcgttcat cgacgtaaaa ttcatgaccg cagaaccagg ctttgttggt
    59881 caggtaagcc accggaatgc gctcgttgac gcggcggatc acgcgttcaa caatacggtg
    59941 tttttcgctg gaggtcagac gcgcggtgcg catatcttcc gggatatcca gcggcaggta
    60001 gagcgatggt aacaccagct gtacggcttc atcccacggg ttatcggtgc cgtgaccgta
    60061 ccagatattt gccgcgctga agcggcttac cgaccagcgc aacatgtcct gaatggtttg
    60121 cagctcattt actgcttcat caacgaaaat tttatccacg tattcctcca gggcatgatc
    60181 gcaataattt cggcggctag tttgccatga agatgacgat aaatcagcat tcacgcgcgg
    60241 tgagtgagga aaaatacgtt taaaacgatc gattgtgcta cgagtcgggt aaactgtagg
    60301 aaaattagaa atagagacag ataaatgaaa aagaaaacaa cacttagcga ggaggaccag
    60361 gctctgtttc gccagttgat ggcggggact cgcaagatta agcaggacac gattgtccac
    60421 cgaccgcagc gtaaaaaaat cagcgaagtg ccggtgaaac gcttgatcca ggagcaggct
    60481 gatgccagcc attatttctc cgatgagttt cagccgttat taaataccga aggtccggtg
    60541 aaatatgttc gcccggatgt cagccatttt gaggcgaaga aactgcgccg cggcgattat
    60601 tcaccggaat tgtttttgga tttacacggt ctgacgcagc tgcaggccaa gcaggaactg
    60661 ggcgcgttga ttgccgcctg tcgccgcgaa catgtgtttt gtgcctgcgt gatgcatggg
    60721 cacgggaagc atattttgaa gcaacaaaca ccgctgtggc tggcacaaca tccgcatgtg
    60781 atggcatttc accaggcacc gaaagagtac ggcggtgatg cggctttgtt ggtgttgatt
    60841 gaagtggaag aatggttgcc gccggagttg ccctgaatgc tggcagcaaa tgccggagca
    60901 gcgtaaacgc cttatccggc ctacaaatat gtgataattc aataaattgc aattcacttc
    60961 gtaggcctga taagacgcat cagagcaata ctacgaactt cagttcattc aatacatact
    61021 gaaaacgaca cgtcgaatca atcggttgcc cactactggt attcacattc accacaatat
    61081 cgtgtagtac tttatccgtt accggatgac gagttggcgt aaccgttacc gttgatttat
    61141 cacaaccgta agaggtaaag gttaacaatt ctgataccgc gcggcagtat gctcctctgc
    61201 catcattatc agaaaaatta ctgccatcac agagtctgcc gccttccatc aggtaggtcg
    61261 aaagcgtggt ggtcactgtt gaccccgtgg actgaattcg taacgcctta ttatggctga
    61321 cataattttc actccaggag atatcgtatg aagcgaccgg tatcgttggg catattgtgt
    61381 tatgcactgt aggggccgta tagcttccaa atccgggtaa atctgtatag gaataaaagg
    61441 atgcgatcca agggcaacca taatcatcag atgcaacggt aattttaaac gtcatatatg
    61501 cacaacgaaa aattttcgta tctatgccag ggcaatttga gttattaatt gtatcaaagt
    61561 cagtcagtac caaagttgga gctgaacccg acccagacca ggaaataacc cgcaacgtat
    61621 cgcctttata cgcaaaagaa tcatatttcc catatgcaac ctcctttggc gttatcaccg
    61681 taacattgaa aacactgctc gctacagcac cggttacctt gtccatggca taataaactc
    61741 tcacactgtc aaaactcgca ctcataagtt tatctccagc ataactctta gatgcgccga
    61801 caacactcag aacgagcagc attaggctcg cgaaaattat ggtccatttt ttcatttcag
    61861 aaatactcaa aggttgccgt cactgtggca ctaaagtctc cggacgttac atctcgcccg
    61921 ctctttgcct gtaaccaggt attaaaattt aggctgttat ttccctgatt cagcgggagc
    61981 gtgacgcctg ttgtattgtt gattggcacc gatgtgcctg ccgcagtttc aataccaatc
    62041 ccagcgccgg atgctgatga cgaggagtca aaggccagaa agccgggtag agcactatct
    62101 tctgttcctg tcaacgttac tttgacatta aacaatgtag aactatgaca atctttcaat
    62161 tgaaacacca atggcactcg ctctgactgc ccaaggttca ttaaatcact ggcggcaatg
    62221 gcaggaaaat gtacttctgc caactggcta ccattaatca ctggcgtaca cgatttactg
    62281 attaaattgc cggtaaagtg caaattatct cctgctattg tggttccact gcccagcaac
    62341 atcaggctac taataaaaaa caacgtacgc tttttcttca ttggtaatct acctgtaacg
    62401 tggcccaggc ctcaaagtcc gcctctggca gaatgacacc actctttttt actggcacag
    62461 ccgttaacac cggtaaatcg gtttcattca ccacaagtgg cgtattgatc gtaaaactct
    62521 gccctgcctg tttgagctga atgcctaaac cagtgatatt gctgcttact gcagcattat
    62581 caaaatcgct tggtgtgcca ctccagctca aggtcatttc cattaatggg tcgcgaaccg
    62641 ccgtatcgca ggtaatggaa tacggcacgg tttgctcata attgctgcca ttaatgtcat
    62701 caataagcac atcctgaaaa gttatttcaa tggtttgagc gttgctaatc gtgcagttag
    62761 gcggctgcac taaataaccg tgaaaagaga cgtttgagag tgccatcgag caaaagcccc
    62821 acagcaaaat aagcgagatc cgtttcattg gtaatccacc acaattgtgg cactggcgtt
    62881 aaattcagct gccgctaacg ttgtaccact ctgtttcacc ggcacagctt ccagaacggg
    62941 aactcccgag gaaaaattaa atggcaacca tgagccagag gttaagtcga gtattgtgtg
    63001 atcactactt ttctgaaccc gaataccaaa cccaggaata cccgtgctga taactgtttc
    63061 accgttaatc acgaccgttg tcgcctgcat ctgtagacgc aggtcatcca ttgccagatt
    63121 gttacaaaca agcgagtagt caatgggacg tttataatca accctattga tcttattaat
    63181 gaatacatta ccaaactcaa ctgccgcacc atttaccgta caaggaggag gggcatcgac
    63241 aagcacccgt aaattcatac ttactgactt actcgctgca actgcaaagc cgctaccaac
    63301 acaacatatc agtaacccag ttaccgccca gacagaacaa gatcctttca tactggctct
    63361 cccggcctca gcctttgcgg ctctgctctt catcgaccta gcaactgtta ccgttgcaag
    63421 tgaaaaagag cggcatccga gcgccatagt cgttgacata ggtcagtacc ggcacgctac
    63481 ccatcttcac attcagcggt gcagaggttt tcggcgacat cactattagc gagaaaccgg
    63541 ctgccggatt gccatttttc tgtgtgctgg cattactgaa aatgacgtaa tacgacgtcg
    63601 ggttattgac ggtaaattcc tgaccgttgc gggttaaagt aatcttatat tgctaggggt
    63661 ttttcatgct gacgttctcc agcgccttcg gtcgccagaa caacttaata cgcgtttgta
    63721 gtgctatttg cagcgtgttg gctttgtttg attttggcgg gatctcgcgc acgttgaagt
    63781 agaacaggct ttcacgatcg gcaggcaatt tgttgatatc cggcattccc tgcactttta
    63841 cctgcccgtt catcattgaa tcgatgcgtt gcaccggtgg cagaacagtt aacggtgaac
    63901 tgatcttgtt acccttttca tcttcgatcc agctttgggc cagataaggc agtttcggat
    63961 cgttattgcg caacgtcacg ctgattgatt tgtcgctttc attaaatatc aaacgggtac
    64021 gatctggcgt tacagaagcc tgggcagaca aactggcagc gctaagcagt aacgctagcg
    64081 tcgtagcacc caatttggca gagcaaagta aatcagacat ggatcattcc ttaatcacgt
    64141 tattggttag ctgaaacaga aacaggggga ttggtgggtg tcacctgttg cgtctgctcc
    64201 tgaatgacag gctttatgtc atcaggaaca accggcgcga cgttgccttt atgctggcat
    64261 ggcagtaaca agccgttaaa cagatcggca ggcagcgggt ccggcaggtt gatatcgcaa
    64321 tgcgcaacac cactccagaa cacactcatg tgttcgccag gtttcacccc agccagataa
    64381 acattgccgt catcatcgac aaggcccact gtctgctcgt tatcattttt tacttctgcg
    64441 ccaaacggag gatggctgcc gtcttgtaaa cgcagcaccg ccatcgcttt ttgaccactg
    64501 atgacggcaa atttgcggta gccaatcgcc ccttcagtta gcgtggcttg taccaccgac
    64561 tgggttgctt cggcgttttc cggcagtttg ttgaggtcga tatacgcctg attgcggtaa
    64621 tagttattga cgtcagaaac gacggcttta ccaaacatat tggtataaac agcagcaccg
    64681 ttaccttcta ccggaacatc ggcaacgcca tcggcatcaa tcaacaagcg tgtcccgccc
    64741 atattctggg tacggtgaag tgcgccaccg tgggcagtaa gcgttgcgcc gccctgtaac
    64801 gacaagcccg cagaggtgta ttgcccttca tggtagttcg cactgaggtc aacctgcgcc
    64861 agcgaaccat catggctgta atagccgtca acgctggtgt gtttgtcact ggtgccgacg
    64921 ttcaactgat agtgagtcgc gtcatcgaca cggctgaaat aaccgacctg actgctgtcc
    64981 gtcccactgc catagtaccg ttatagctaa cggtactgtt gtcgccccac ggcatactga
    65041 gcgaaatgta catgcctttg tcggcctggt tgtcatactc gtaacggtag ccagtcatcg
    65101 agatgctgac attacgaata ctgcccatat tgaaatagtg cgagagcatg atgttgtagt
    65161 ttgtctgctc ctcgcgatcc cagtaggtat ggcgggtata gttgagataa accgaaacac
    65221 ccgcatcgcg gaagttctgg ttataagtgg cggtgtacat ctctttgtcg ttgcccgtgc
    65281 gtaccattcc gctgtcactg gcatccagat actcgctcat ggtcataaag ttctcttccg
    65341 agaagcgata tccagcaaaa gtaacgcggc tgttgagctg gtcaaagtct ttggaataac
    65401 tcacacggaa ggagttaccg tccagcgaac ctttgccata agcggtgtct ttatccagtt
    65461 tggtgtgcga gtgagtaaca tcaaacgcga ccgcgccgaa tgtagacaaa tcgcgaccga
    65521 caccaagcgc cgcagactga tagttttcat ctcccagtgc gccgccatat aacgaccagc
    65581 cgttagcgat cccccaggaa gcttcagcac cagaaaaaaa ttcaccctcg acatggtgtc
    65641 cccactcttg cggacggccc atcatgatct tatagcgaac ctgacctgga cgagtgaggt
    65701 atggcatcga ggcggtgctg atgtcatatt cctgcacctg gccgttctgt tcttcaatgc
    65761 gaatatgcaa cgtaccggag acagaatcac caagatcctg aatacgaaac ggtccagccg
    65821 gaacctgcgt ttcgtaaatc acacgcccca tctggctgac ggtcactttt gctgtggtgt
    65881 gtgccacgcc ggaaatgtct ggcgcgtagc cgcgcagatt gggaggcaac atttgatcgt
    65941 cagtactgac actgccacca acatagttaa aaccatcaaa aatatcggat ctgaggtaat
    66001 cctcgcccag cgccagtttg gcttttaatg atggtaacgc ccgcctggca tagtagcgac
    66061 tccactccca ttttttttga gtttcatcgc cgctgaattc atcgtcatca ttactgcgag
    66121 tatgttgata gttagtctgc cagtcagcac gcatacgcca cggccctagg ttaaccccaa
    66181 ccgtcccgtt gccgctgatc tcgttactat catcaccgcc attttcttcg tgccgggttt
    66241 gtgcgttgat gctgtagtcc gcgacgatcc cggagatgcc gtcatcccaa cgtgaaggcg
    66301 gatcccagtc gggataagta tattcgaggt aagcctgtgg cagtgaaatg actaatgcgg
    66361 actggcttaa atcagcctta atttccatgc cttccagttg accggatttc aggcatttag
    66421 catcgtggct ccattgcaga tttttcgcca cgtcttcttt taaaccaaac tgcgctacca
    66481 gttccggtgt cagacaagca tagcttttgc tcgcgtcatc ttcaccagca taccagtaaa
    66541 tatcgtactc ttccgccaat ggctgtttat ttagttgaac ctgtaaattg tattttccgg
    66601 gctcaacata tccctagctg gaaaaacgct tcagatcaat ttttgtgtcg ccttttaatt
    66661 ccagaaaacg ggaatcaaac tgaatgtcgt cttcagccca gacactgcta taactccctg
    66721 acattgccag cgcaatgcac cacggaagaa tccgtaatcg aaaaagagaa tggtcaggca
    66781 tacacgtttc tatccgtttt tattcttgtt cggtcaaaat acatttaaat cccccatagc
    66841 agtgtcgcta tgggggtatg ccttacaggt aagtaatctg gaaggtggta tttgcttcaa
    66901 aattacccag atctggcgca tcagctgcgc cgaccagcca ggcgttaaag ttcagtgtct
    66961 gcttcgcttt gcctttgttg gtagaagtgt ctttcactat tttctggtca atacccatgc
    67021 cgcttttgta agaggtaccc agagagtcac cgatcgccag gctaacattg ttaaatgccg
    67081 caccggtatc ggtattgaaa atggtgtaat aattgccgct atttgcagaa gaaacggtac
    67141 cagtaaaggt agtggtcatt gtttcctgag tatcaaatac gcaatcttgc agacgaatct
    67201 ggaaactttt cggcgtggtg gtgccgttat ttttcagttt ttcggcaccg atatcaccca
    67261 tatcaacttc cagtttgtga tcgtctggaa cgatagaaca cactgagtct tcaatggtgc
    67321 cgtaaaaacg tgcagtaccg ttgttacccg cagcgattgt tgccgtagag gtgaacacgc
    67381 ccatcaccat tgccgcagca atagctgttt taacaaactt actcattatt tgtccttaaa
    67441 ctataaataa ttaaaaatat aaacattgca atacattgat tcagtcaata gccaatgttt
    67501 atacccagcc attgaatacg gcatgcgacc caaaaagaac aggtcgtaga aaattgtatt
    67561 cattggtgtg gggccgcata gtgatgtttc cacacccctg atccaattta attttactaa
    67621 agagtaaatg tagatttaat taatatattg acagaagaaa ggttatctta aggacatcct
    67681 taaaaaatac ggaataagta tttagaaaaa atagaaagta tatcaataat gaatatcaac
    67741 agcttaaaaa acaaacaatg aacatataat gcgattggca tttatccact tttgacaata
    67801 taatatataa acactatagc cagaatttaa tcaagtttca attacatcct gcctgattat
    67861 tcaactctat tttctctatt atctgtatta ataaatgaca atgagtgtca aacagatatt
    67921 attcatttca acatgtaact ttgggaaaaa tcaagatacg cgcagaagaa taatttccga
    67981 atgaatgtag aaagtgtttt atttatagat aaggagagtc ttcgatagta gtgaggttga
    68041 cagatatccg ctgcctaatg agcaattatg cccaatgaat ctacctcatt gggcataatt
    68101 tggaacacat cagatagctt ttgccatctt cagattacac gggctcatct gccagttgaa
    68161 tgtaccgttt ccgctttcat caagcgttac gctggcaatg gccgaggtgg taaacatagg
    68221 cggcgtttcg cccggacata actcggcaac cagatacccg actaacggta agtgagagat
    68281 caccagtacc gaagcaacac cttcattggt caacgcctgc aaataggcac tgaccagacc
    68341 aacatcgccg cagggcgtta actccggcag aacttctgca ctggaaggca ggttcagaca
    68401 atcccctact tcttccagtg tttgctcggc tcgcaggaac gggctcacca gaacgcgttc
    68461 gatttccact ttttgacctt tcagccagtt cgccatcagg cgagattcgt cacaaccatt
    68521 agtggtcaga ggacgaaccg aatcactggc ggaatcgagg gctgcgtcgc cgtgacgcat
    68581 gataaaaact tgcatattgc accgcttttg ttaaccagat tcgcccgttt tctttcatct
    68641 gaaaccagag aaagaaaacg gtggccggca ttgtgcctta tccattcacc gaatgaaacg
    68701 ctgtttttta cctcaatggc gtaagtatag tcaatatgcg tttacatttt gaccaacact
    68761 ccgccattca gcgcggattc atatagcttt gaccttctta ttgcaggtca gttgcagttg
    68821 ttttccaaaa actttcccca cgcgcgccca tctcgaccaa acgctcgcaa ggggtaaaac
    68881 gggaaccata ctgcgtggcc agtcgttgca ttattgcaac cacttcgccc gcgccgagag
    68941 aatcgatata gcggaacggt ccaccgagaa atggcggaaa accaatgcca aataccgcac
    69001 caatatcccc gtcacgcacg ctacggataa cctgctcatc aacacaacgt actgcttcat
    69061 tcagcatcaa catcacacac cgttcagcaa cctgcggtgc ggagagtcac ccctgccctt
    69121 gtgcgccaat gagcgggtaa atggcaggat cgacctgttt tttgctttta cgccctttct
    69181 gaccataaag atagaaaccc cgaccatttt ttctgccttt gcgatcgtcg ttcaaaattg
    69241 aagaaacaac atttgcaggc gcgctaaaac gttctccata agcggcttcc agtacaggaa
    69301 taattttagt cccggtgtcg attcctacct catccaaaag ttggattggg cctaccggaa
    69361 aaccaaattt cactagcgcg gcatcaatgt gctctacccg ttcaccttcg gtcagcatgc
    69421 ggatagcttc attaatgtaa ggcgctaaga tgcgattgac gtaaaacccg gctttgtcac
    69481 gcacgacaat gggcgttttg ccctgttttt tcgccagttt taccgtagtg gcgatggttt
    69541 gcgccgatgt acccgcatga ggaataatct ctaccagcgg cattttttcc accggactga
    69601 agaaatgcag gccaataact tgctcaggtc gcgcggcgtg agcggcgata tcaccaatcg
    69661 gtaaagatga cgtgttcgaa gcaaaaatgg tatgagcggc gcaattttgc tccacttccg
    69721 ccaccatctg ttgttttaat tcgagatttt caaacaccgc ttcaataatc agatcgcgat
    69781 gggcaaagcc gcgatagtcc gtcgttccgg agattaatgc cagctgtttg tcacgttcgc
    69841 tggctttgag atgacgacgg cgaactttgc cctccagctg atcccaactg tacttcagcg
    69901 catgatttat gccctgcggg ttgatatctt taattctgac cggaagcccc gctttacaag
    69961 cagtgacata agcaataccg ccgcccatca agccaccacc taaaattccc acgctgttta
    70021 atgccgcagg cggtgcatca ctaccgggat ctttcttcac gtccgtactg gcaaaaaaga
    70081 tactacgcaa agcttgcgat tgcggcgtca tcgccagttc gccaaaggcg cgagcttcag
    70141 cgtcatagcc gctgctggtg ccctgcgcta atcccgtttc aacaacctcc aggatgcgtt
    70201 ctgtcgccgg ataattgcct tgagttttgt gttctgtttt cttgccgacc attttgaaca
    70261 gcagcgcacg acctaacggc cccgccagaa tacgctcgcg tacaggtaga gggcgggaag
    70321 atgggcgatc cttcttcgcc agctcaacag cggcttccag caaaatggag tgcggaacaa
    70381 cgtcatccac cagccccagc tttaatgcct gtttcgcccg aagttgtttt ccggtgagaa
    70441 tcatctctaa tgctgtactg acgcctatca gacgcggtaa acgctgggtg ccgcctgaac
    70501 cgggtaacaa tccaagttgt acttcagaca aaccgagcac cgttttagga tcgtcagtac
    70561 aaacgcgacc gtggcacgcc agcgccagct ccagcccacc gcccaggcaa gcgccatgaa
    70621 tagccgcgat aaccggaatg ggcaaagcat gaatctccgc catcaactgt tggccctgcc
    70681 gcgccagtgc ttccgcttct tgcgccgttt tgcagttgcc aatcatgttg atgtctgctc
    70741 cggcgataaa attgtccggt ttcgcggaga taaacaccac gccgcgcaac tctttgtttt
    70801 cacggagttg cttaataatg gcgcgcacct gcgaggcaaa ttccgccttc agggtattca
    70861 ttttctcatc cggtacgtcg atggtgatga tagcaatgtt gtccagacga acattaagag
    70921 taaacgctga tgccatttcc attattccgc ctccagaacc attgccgcgc caagcccacc
    70981 ggcagcacag gcggtaacta aaccaaatcc accgccgcga cggcgaagtt catgcaatgt
    71041 ctgggtaatc atccgcgcgc cggtcgccgc gaagggatgt ccgtaagcaa tcgaaccgcc
    71101 aagcacgtta aatttgctat cgtccacttc gccagtggca tgtgcacgcc ccagtacttc
    71161 acgagcaaaa cgttcactac ccagcaactg aatattcgcc agtgtctgag ctgcaaaggc
    71221 ttcgtgcata tcgatcaatg tcagttcgct catcgtcaaa ccggcacgtt ccagcgccag
    71281 cggtgttgac caggctggac cgagcaacat gtcctgccag acatcaatcg cagtaaatgc
    71341 gtagctgcgc agatacccca gcggcaccag ccctaattct ttcgcccggg attcagtcat
    71401 caggatcacc gctgccgcgc catcggtcag cggcgtactg tttgccgccg ttaccgttcc
    71461 gtgtttgcga tcaaacgccg ggcgcagctt tgcgtaatcg gcaagcgagg aattaccgcg
    71521 aatattgttg tcttcgacaa gcggttgttt ataaggaggg ataaaggcag tcatcacctc
    71581 ttctttgagt tttccgtctg accatgcctg agcggcacgc tgatgcgaac ggtgcgctaa
    71641 tgcatcttgc tgttctcggg tgatgccgta ggttttcgcc atttgttcgg cggtgtcgcc
    71701 catccgcaag ccagtagaat attctgctac cgcaggcggt acgggcatta agtcgcgcaa
    71761 acgcaggcga gagaagagtt tcaggcgctg gctcatggtt cgagctttgt tgacatcaac
    71821 cagcacgtgc gccagttttt tactgacgcc aattggcaat acggaagagg aatctgcccc
    71881 accggcaatc cccgctcgga tagttcccgc catcaggctt tctgcgacgt ttgcaaccgc
    71941 ctggaaactg gtagcgcaag cgcggctgac gctgtaagca tcggtatgca cattcattcc
    72001 ggtaccgaga acaatttcac gcgcaatgtt gggggcttca ggcatttgta cgacctgacc
    72061 aaagaccagc tgttcaatca cttcagcggg gatctcgctg cgtgccagca gttcgcctac
    72121 caccatcttc cctaaatcaa ccgtgggaat gccatgaaaa gccgtcgcct ggcgggcaaa
    72181 aggcgtacgt aaaccgctaa caatggcgat acgatcgccc tggcgggtaa ccagtggtaa
    72241 aacctgaccc ataacactcc cctgtaaaaa aataaataaa gtggtctgac ctgatcatag
    72301 tcttaaccat ttttttacat ttagccaagc ggagaaaaag gaaagtggga gctatgacac
    72361 agtgaagaca gaagaaagaa aagaaaacgc ccctgccagc aactgacagg ggcgtacgta
    72421 cccgagagga attaacgcag acccagctgg aagatcagca tttcagcttc gcaggcaaaa
    72481 gtgaaatcga tatccaggcg cacaccgtca gactcttcag tgaaagtcgg ggagattttg
    72541 cacggttcgg attccacgct acgggatttt tcagtcagcg ccgccagcgt ttgttctgct
    72601 tctgcgcggt ttgcaaacac gcggctgtaa gacgcggtgc agtcggagtt gtccataatg
    72661 gtgccaacat ccatacagca gcaaaccggg gtttcatcag cactacattt actcatcgtt
    72721 gatttcctct gtatgtgcac ccaaggtgcc agataaacgt tgtggatatt ttacgcttcc
    72781 ggaaagtgct gctccagttg ttaattctgc aaaatcggat aagtgaccga aatcacactt
    72841 aaaaatgatc taaaacaaaa ttcacccgaa tccgcgagtg cgcaacctcc aaattttgcc
    72901 agctggatcg cgtttcttag atcatatttg aaaaaagatg gaaacatact tgcaacattc
    72961 tagctggtcc gacctatact ctcgccactg gtctgatttc taagatgtac ctcagaccct
    73021 acacttcgcg ctcctgttac agcacgtaac atagtttgta taaaaataaa tcattgaggt
    73081 tatggtcatg agccagaaaa ccctgtttac aaagtctgct ctcgcagtcg cagtggcact
    73141 tatctccacc caggcctggt cggcaggctt tcagttaaac gaattttctt cctctggcct
    73201 gggccgggct tattcagggg aaggcgcaat tgccgatgat gcaggtaacg ttagccgtaa
    73261 ccccgcattg attaccatgt ttgaccgccc gacattttct gcgggtgcgg tttatattga
    73321 cccggatgtg aatatcagcg gaacgtctcc gtctggtcgt agcctgaaag ccgataacat
    73381 cgcgcctacg gcatgggttc cgaacatgca ctttgttgca ccgattaacg accaatttgg
    73441 ttggggcgct tctattacct ctaactatgg tctggcaaca gagtttaatg atacttatgc
    73501 aggcggctct gtcgggggta caactgacct tgaaaccatg aacctgaact taagcggtgc
    73561 gtatcgctta aataatgcat ggagctttgg tcttggtttc aacgccatct acgctcgcgc
    73621 gaaaattgaa cgtttcgcag gcgatctggg gcagttggtt gctggtcaga ttatgcaatc
    73681 tcctgccggg caaactcctc aagggcaagc attggcagct accgccaacg gtatcgacag
    73741 taataccaaa atcgctcatc tgaacggtaa ccagtggggc tttggctgga acgccggaat
    73801 cctgtatgaa ctggataaaa ataaccgcta tgcactgacc taccgttctg aagtgaaaat
    73861 tgacttcaaa ggtaactaca gcagcgatct taatcgtgcg tttaataact acggtttgcc
    73921 aattcctacc gcgacaggtg gcgcaacgca atcgggttat ctgacgctga acctgcctga
    73981 aatgtgggaa gtgtcaggtt ataaccgtgt tgatccacag tgggcgattc actatagcct
    74041 ggcttacacc agctggagtc agttccagca gctgaaagcg acctcaacca gtggcgacac
    74101 gctgttccag aaacatgaag gctttaaaga tgcttaccgc atcgcgttgg gtaccactta
    74161 ttactacgat gataactgga ccttccgtac cggtatcgcc tttgatgaca gcccagttcc
    74221 ggctcagaat cgttctatct ccattccgga ccaggaccgt ttctggctga gtgcaggtac
    74281 gacttacgca tttaataaag atgcttcagt cgacgttggt gtttcttata tgcacggtca
    74341 gagcgtgaaa attaacgaag gcccatacca gttcgagtct gaaggtaaag cctggctgtt
    74401 cggtactaac tttaactacg cgttctgata acgcgttcgc ctagataaag tcacctgcta
    74461 tgcaggtgac tttaactccc ccacttcacc gaaagtagtt tcctcattgc ttgcctcctg
    74521 agttttgcaa acagcctgat ggcagcttgc cctttcaaat cagtaagtag tatccacaga
    74581 caaacctgaa gggaaatgca tattttcaag cgttctgctc gtccttctca aagagtttac
    74641 ttttctgcat ttccaggata ctcaaccccc ctggctattg tgcgcttata cactcaaatt
    74701 aaagataggt tctaaataaa tgagcgtttt ttgatagtct atttcattag gtaatatata
    74761 tttgtaacaa atcaatcaac atggaataaa atcatgctac catctatttc aatcaacaat
    74821 accagcgcag cttacccaga atccatcaat gaaaataaca atgatgaaat taatggatta
    74881 gtacaagagt tcaaaaacca tttttaatgg taaggaagga ataagcacct gtattaaaca
    74941 tctacttgag cttataaaaa acgccatacg agtaaacgac gatccttata gatctaatat
    75001 taataattcc tcagttactt atattgatat tggctccaat gatacagacc atattactat
    75061 tggtatcgac aaccaagaac caatagaatt acctgcgaac tataaagaca aagaactcgt
    75121 ccgtactatc attaatgaca acatagttga gaagactcat gatatcaata acaaggaaat
    75181 gatcttcagc gcattaaaag aaatatatga tggagatcct ggttttatct tcgataagat
    75241 atcacacaaa ctcagacata cggtaacgga atttgatgag agcgggaaaa gcgaaccaac
    75301 ggacttattt acctggtacg gtaaagataa aaaaggcgac tctctcgcta ttgtaattaa
    75361 aaataaaaac ggaaatgatt acttatttct cggttactac gatcaggacg actaccacat
    75421 tcaaagagga attcgtatta atggtgatag tctcacccaa tattgtagtg aaaacgccag
    75481 gagtgcttca gcgtggtttg aaagcagtaa agctatcatg gcagaatcat ttgcaactgg
    75541 ttccgatcat caggttgtaa acgagctcaa cggggaaaga ctgagagaac ctaacgaagt
    75601 ttttaaacgt ttaggtcgag caataagata taattttcaa gtggacgatg caaaatttag
    75661 acgcgataat gtaaaagaaa taatttctac tttattcgct aacaaagttg acgttgacca
    75721 tcctgaaaat aaatacaaag actttaagaa tctcgaagat aaagttgaaa aaagacttca
    75781 aaatcgccag acgaaatatc aaaatgaaat taatcaacta tctgcactag gtgttaattt
    75841 tgatgatatt taaaattatt tttccagttt tttgattttt caacgcctga atatcggaaa
    75901 tagtcagtga caaacatcag tatttttaca tcattacgtc cgggtcattg tcggtttatc
    75961 tccttttact tgtggtaatt gagggataac aacaaaaaag gtgagttttg cgactcacct
    76021 tttttatttg tttcttattc agaatcgata tcttttaaat catcctgaat cgcttgcgcg
    76081 ttcggatttt cctgcggttt gagttcgccg ccattagcga tgaaatcatg acgctggaag
    76141 tacgcttcgc gcaccataat ataaggatcg gacgactgac gcagcagacc atcggaatcc
    76201 agcagctgag cgcgggtttc gatcccttca agcgtccatt tacccacaga catcggccag
    76261 gtcagccagg aaagaaccgg gtaaagagca tccgccatat caccaccgtc atcacgcagc
    76321 gtgaagctac cgtagaacgg taactgaacg taaggcccat aacccacgcc ataatgacca
    76381 agcgtactac cgaagcggtg aggttcagtc cgttgcagtt tcgggttcgc catccctgca
    76441 acatcaataa agccgcccat ccccaaaatg gtgttcagga aaaagcgggt aaagtggacc
    76501 atcccctgat aagggtcgcc ctgcaagaag tagttaacca tcaccgcagg ttcttcaagg
    76561 ttgccagtaa agttgctcaa accattacgc gccggttgcg gaacataatc acgccaggcg
    76621 acagcgaccg gtcgaacaat atacgggtct aatacgttga agttgaagtt gtacatggtg
    76681 cggttgaacc cttctaacgg gtcagaacgc ccttgctgat ctgtaccgga actcgcacac
    76741 cccaccagaa gcgtagttcc cagagcaagc gccgacaggc gaagcttcat aaatgtctcc
    76801 ctgttttttt atggcttatg cagtttgcca tccatgacgg aacgataccg tatccgcctg
    76861 tttaggtgtg ggcgattgta acagcacgtc aactgatgtc cagacgccct gatttgctga
    76921 tttgatcata gcctggtaat cgccgccctg taggctactt gattctatag aaacagaaaa
    76981 aggcaaacgc agccttttct acattttcag agtaactccc gccagttgcg agcaaaaaag
    77041 ccgctacgct ttagctatac gtgctaattc aagagaagag accatggaca acgacaaaat
    77101 tgatcaacac agcgacgaaa ttgaagttga gagcgaagaa aaagagcgcg gcaaaaaaat
    77161 agaaatagat gaagaccgac tcccctcccg agcgatggca attcatgagc atatccgcca
    77221 ggatggtgaa aaagagctag aacgcgacgc aatggcgcta ctgtggtcag ccattgcggc
    77281 gggtctgtcg atgggcgctt cgttactggc aaaagggata tttcatgccg aactggaagg
    77341 tgtgccgggc agcttcttgc tggagaatct cggttatacc tttggtttta ttatcgtcat
    77401 aatggcccgc cagcaattat ttaccgaaaa caccgtgact gcggtactac ccgtcatgca
    77461 aaaaccgaca atgagcaacg ttggcttact tatgcggtta tggggagtcg tgctgctggg
    77521 taatattctc gggactggta ttgctgcgtg ggcatttgaa tatatgccta tcttcaatga
    77581 agaaactcgc gatgcatttg tcaaaatcgg catggatgtg atgaagaaca cccccagcga
    77641 gatgtttgcc aacgcgatca tttccggctg gctgatcgcc actatggtat ggatgtttcc
    77701 tgcagcaggt gcggcaaaga ttgtggtgat tatattgatg acctggctta ttgccctggg
    77761 tgacaccacc catatcgtgg tcggttctgt tgaaatcctc tatctggtgt ttaacggtac
    77821 gctgcactgg agcgatttca tctggccctt cgcactacct actttagcgg ggaacatctg
    77881 cggcggcacc tttatcttcg cgttaatgag tcatgcacag attcgtaacg acatgagcaa
    77941 caagcgtaaa gcagaagcac gccaaaaagc agaacgtgcg gaaaacatta agaaaaatga
    78001 taaaaaccca gcataaatgg cgagggttta agcaatcgag cggcagcgta cttaccccgc
    78061 agtccattag cgggtatact cgccgcattg tcctcttagt taaatggata taacgagccc
    78121 ctcctaaggg ctaattgcag gttcgattcc tgcaggggac accatttatc agttcgctcc
    78181 catccgtacc agtccgcaaa atcccctgaa tatcaagcct tccgtatatt cacagttcgt
    78241 catggttcgc gtcagatcgt tgacagccgc actccatgac gggtaaaaag tggataaaat
    78301 aattttaccc accggatttt tacccatgct caccgttaag cagattgaag cagcaaagcc
    78361 gaaagaaaaa ccataccgcc ttctcgatgg taatggcctg tacctttatg tccctgtatc
    78421 cgggaaaaag gtatggcagc ttcgctacaa gattgacggt aaggagaaaa tcctgaccgt
    78481 cggaaaatat ccgcttatga ctttgcagga ggcaagggat aaagcatgga ctgcgaggaa
    78541 agacatctcg gctggcatcg atccggtaaa ggcgaaaaag gcttcgtcta acaacaattc
    78601 atttagtgcg atttacaagg aatggtacga acacaagaag caagtctggt cagtagggta
    78661 tgcgactgaa cttgcaaaaa tgtttgatga cgacatttta cccatcatcg gcggccttga
    78721 gattcaggat attgagccga tgcaactgct ggaagtaatc cgcagatttg aagatcgcgg
    78781 tgca
//
LOCUS       DAJTQY010000003        75758 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.003, whole genome shotgun sequence.
ACCESSION   DAJTQY010000003 DAJTQY010000000
VERSION     DAJTQY010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 75758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 75758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..75758
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.003"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(99..319)
                     /locus_tag="OO349_000164"
                     /pseudo
     CDS             complement(99..319)
                     /locus_tag="OO349_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403028.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(312..533)
                     /locus_tag="OO349_000165"
     CDS             complement(312..533)
                     /locus_tag="OO349_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5865388.1"
                     /translation="MHNKTTPNAAAAALTTLMHALIDIECTAELAQGEEQKDRTQFAL
                     ECIRYIATRSLNDAKNILVADCENGGGYA"
     gene            complement(523..723)
                     /locus_tag="OO349_000166"
     CDS             complement(523..723)
                     /locus_tag="OO349_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HCR5865389.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSAGESQIIHVTAWTEREARS
                     RCPSGCVAVFAARIRQGETYAQ"
     gene            853..1032
                     /locus_tag="OO349_000167"
     CDS             853..1032
                     /locus_tag="OO349_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5865390.1"
                     /translation="MSTHKNERRGNPPFQFRLDPDLREKMEKAQQQDGDDSLAAWIKR
                     IIRKELQQRGIEPKG"
     gene            1157..1486
                     /locus_tag="OO349_000168"
     CDS             1157..1486
                     /locus_tag="OO349_000168"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5865391.1"
                     /translation="MNQNDSRRVLLPLRSTLKNTHQRRELRSYGNALNQTDCIAHQPD
                     SGYHPRAYVWDHTHKRRSGLSVQKGGSISGPLFLCLKNPNFVVFQFHQGERIPAHVLA
                     YIQSSWL"
     gene            complement(1536..1724)
                     /locus_tag="OO349_000169"
     CDS             complement(1536..1724)
                     /locus_tag="OO349_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCR5865392.1"
                     /translation="MLSTDRFIREKECEKLTGLSRSCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            complement(2099..3327)
                     /locus_tag="OO349_000170"
                     /pseudo
     CDS             complement(2099..3327)
                     /locus_tag="OO349_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            3576..4697
                     /gene="roxA"
                     /locus_tag="OO349_000171"
                     /gene_synonym="ycfD"
     CDS             3576..4697
                     /gene="roxA"
                     /locus_tag="OO349_000171"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HCR5865393.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(4746..5972)
                     /gene="pepT"
                     /locus_tag="OO349_000172"
     CDS             complement(4746..5972)
                     /gene="pepT"
                     /locus_tag="OO349_000172"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HCR5865394.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVILEGMEK
                     AVQVIVRIAELTAQRK"
     gene            6222..7358
                     /gene="potA"
                     /locus_tag="OO349_000173"
     CDS             6222..7358
                     /gene="potA"
                     /locus_tag="OO349_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HCR5865395.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            7342..8199
                     /gene="potB"
                     /locus_tag="OO349_000174"
     CDS             7342..8199
                     /gene="potB"
                     /locus_tag="OO349_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HCR5865396.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            8196..8990
                     /gene="potC"
                     /locus_tag="OO349_000175"
     CDS             8196..8990
                     /gene="potC"
                     /locus_tag="OO349_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HCR5865397.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            8987..10033
                     /gene="potD"
                     /locus_tag="OO349_000176"
     CDS             8987..10033
                     /gene="potD"
                     /locus_tag="OO349_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HCR5865398.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(10189..11010)
                     /gene="cobB"
                     /locus_tag="OO349_000177"
     CDS             complement(10189..11010)
                     /gene="cobB"
                     /locus_tag="OO349_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HCR5865399.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADVFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLEGL"
     gene            complement(11026..11937)
                     /gene="nagK"
                     /locus_tag="OO349_000178"
     CDS             complement(11026..11937)
                     /gene="nagK"
                     /locus_tag="OO349_000178"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HCR5865400.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGKLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANYFALSEAWDDEFTQYPLVMGLILGTGVGGGLVFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVSLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPCHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(11966..13210)
                     /gene="lolE"
                     /locus_tag="OO349_000179"
     CDS             complement(11966..13210)
                     /gene="lolE"
                     /locus_tag="OO349_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HCR5865401.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VVIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(13210..13911)
                     /gene="lolD"
                     /locus_tag="OO349_000180"
     CDS             complement(13210..13911)
                     /gene="lolD"
                     /locus_tag="OO349_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HCR5865402.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLLNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSCALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(13904..15103)
                     /gene="lolC"
                     /locus_tag="OO349_000181"
     CDS             complement(13904..15103)
                     /gene="lolC"
                     /locus_tag="OO349_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HCR5865403.1"
                     /translation="MYQPVALFIGLRYMRGRAVDRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAAN
                     ITGWRLWLDEPLRVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            15365..16438
                     /locus_tag="OO349_000182"
     CDS             15365..16438
                     /locus_tag="OO349_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HCR5865404.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            16566..20012
                     /gene="mfd"
                     /locus_tag="OO349_000183"
     CDS             16566..20012
                     /gene="mfd"
                     /locus_tag="OO349_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HCR5865405.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGILIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKARNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            20155..21117
                     /gene="ldtC"
                     /locus_tag="OO349_000184"
     CDS             20155..21117
                     /gene="ldtC"
                     /locus_tag="OO349_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HCR5865406.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPASSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(21200..21412)
                     /locus_tag="OO349_000185"
     CDS             complement(21200..21412)
                     /locus_tag="OO349_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015705050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR5865407.1"
                     /translation="MSFANFAAVEVQSTPEGQQKVGTISANAGTNLGSLEEQLAQKAD
                     EMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            21697..22329
                     /gene="comR"
                     /locus_tag="OO349_000186"
     CDS             21697..22329
                     /gene="comR"
                     /locus_tag="OO349_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HCR5865408.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(22391..22930)
                     /locus_tag="OO349_000187"
     CDS             complement(22391..22930)
                     /locus_tag="OO349_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCR5865409.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(23157..24461)
                     /gene="ndh"
                     /locus_tag="OO349_000188"
     CDS             complement(23157..24461)
                     /gene="ndh"
                     /locus_tag="OO349_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HCR5865410.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAIKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISTAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(24869..25411)
                     /locus_tag="OO349_000189"
     CDS             complement(24869..25411)
                     /locus_tag="OO349_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR5865411.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(25434..26459)
                     /gene="nagZ"
                     /locus_tag="OO349_000190"
     CDS             complement(25434..26459)
                     /gene="nagZ"
                     /locus_tag="OO349_000190"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HCR5865412.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(26470..27294)
                     /gene="thiK"
                     /locus_tag="OO349_000191"
     CDS             complement(26470..27294)
                     /gene="thiK"
                     /locus_tag="OO349_000191"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HCR5865413.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKGGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(27275..27916)
                     /gene="lpoB"
                     /locus_tag="OO349_000192"
     CDS             complement(27275..27916)
                     /gene="lpoB"
                     /locus_tag="OO349_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HCR5865414.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDRTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(27931..28308)
                     /locus_tag="OO349_000193"
     CDS             complement(27931..28308)
                     /locus_tag="OO349_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HCR5865415.1"
                     /translation="MRKGCFGLVSLVLLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(28311..28670)
                     /gene="hinT"
                     /locus_tag="OO349_000194"
     CDS             complement(28311..28670)
                     /gene="hinT"
                     /locus_tag="OO349_000194"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HCR5865416.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            29009..31194
                     /gene="fhuE"
                     /locus_tag="OO349_000195"
                     /pseudo
     CDS             29009..31194
                     /gene="fhuE"
                     /locus_tag="OO349_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
     gene            complement(31254..32687)
                     /gene="ptsG"
                     /locus_tag="OO349_000196"
     CDS             complement(31254..32687)
                     /gene="ptsG"
                     /locus_tag="OO349_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HCR5865417.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFFFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLLPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(32982..33779)
                     /locus_tag="OO349_000197"
     CDS             complement(32982..33779)
                     /locus_tag="OO349_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR5865418.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(33790..34794)
                     /gene="holB"
                     /locus_tag="OO349_000198"
     CDS             complement(33790..34794)
                     /gene="holB"
                     /locus_tag="OO349_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HCR5865419.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(34791..35432)
                     /locus_tag="OO349_000199"
     CDS             complement(34791..35432)
                     /locus_tag="OO349_000199"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HCR5865420.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(35422..36444)
                     /gene="yceG"
                     /locus_tag="OO349_000200"
     CDS             complement(35422..36444)
                     /gene="yceG"
                     /locus_tag="OO349_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HCR5865421.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTLMYTANTTDVALLKRVHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(36447..37256)
                     /gene="pabC"
                     /locus_tag="OO349_000201"
     CDS             complement(36447..37256)
                     /gene="pabC"
                     /locus_tag="OO349_000201"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HCR5865422.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIL
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVTFSSATLYEYLAPLCERPN"
     gene            complement(37376..38617)
                     /gene="fabF"
                     /locus_tag="OO349_000202"
     CDS             complement(37376..38617)
                     /gene="fabF"
                     /locus_tag="OO349_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HCR5865423.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(38704..38940)
                     /gene="acpP"
                     /locus_tag="OO349_000203"
     CDS             complement(38704..38940)
                     /gene="acpP"
                     /locus_tag="OO349_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR5865424.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(39151..39885)
                     /gene="fabG"
                     /locus_tag="OO349_000204"
     CDS             complement(39151..39885)
                     /gene="fabG"
                     /locus_tag="OO349_000204"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HCR5865425.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(39898..40827)
                     /gene="fabD"
                     /locus_tag="OO349_000205"
     CDS             complement(39898..40827)
                     /gene="fabD"
                     /locus_tag="OO349_000205"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCR5865426.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDAPIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(40843..41796)
                     /gene="fabH"
                     /locus_tag="OO349_000206"
     CDS             complement(40843..41796)
                     /gene="fabH"
                     /locus_tag="OO349_000206"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HCR5865427.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLDIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(41864..42934)
                     /gene="plsX"
                     /locus_tag="OO349_000207"
     CDS             complement(41864..42934)
                     /gene="plsX"
                     /locus_tag="OO349_000207"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HCR5865428.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(43015..43188)
                     /gene="rpmF"
                     /locus_tag="OO349_000208"
     CDS             complement(43015..43188)
                     /gene="rpmF"
                     /locus_tag="OO349_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HCR5865429.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(43240..43761)
                     /gene="yceD"
                     /locus_tag="OO349_000209"
     CDS             complement(43240..43761)
                     /gene="yceD"
                     /locus_tag="OO349_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HCR5865430.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            43960..44544
                     /gene="yceF"
                     /locus_tag="OO349_000210"
     CDS             43960..44544
                     /gene="yceF"
                     /locus_tag="OO349_000210"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HCR5865431.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(44656..45615)
                     /gene="rluC"
                     /locus_tag="OO349_000211"
     CDS             complement(44656..45615)
                     /gene="rluC"
                     /locus_tag="OO349_000211"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HCR5865432.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            46188..49373
                     /gene="rne"
                     /locus_tag="OO349_000212"
     CDS             46188..49373
                     /gene="rne"
                     /locus_tag="OO349_000212"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HCR5865433.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQSNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACTSPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(49569..50522)
                     /gene="flgL"
                     /locus_tag="OO349_000213"
     CDS             complement(49569..50522)
                     /gene="flgL"
                     /locus_tag="OO349_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HCR5865434.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEANGDYVGGTESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKEIAAAALDKTNRGLKNSLNNVLTVRAELGTQLNKLESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(50534..52176)
                     /gene="flgK"
                     /locus_tag="OO349_000214"
                     /pseudo
     CDS             complement(50534..52176)
                     /gene="flgK"
                     /locus_tag="OO349_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
     gene            complement(52242..53183)
                     /gene="flgJ"
                     /locus_tag="OO349_000215"
     CDS             complement(52242..53183)
                     /gene="flgJ"
                     /locus_tag="OO349_000215"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HCR5865435.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKASEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQTLSQLVQKAVPRNYDDSLPGDSRAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGQVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQVLQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(53183..54280)
                     /gene="flgI"
                     /locus_tag="OO349_000216"
     CDS             complement(53183..54280)
                     /gene="flgI"
                     /locus_tag="OO349_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HCR5865436.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(54292..54990)
                     /gene="flgH"
                     /locus_tag="OO349_000217"
     CDS             complement(54292..54990)
                     /gene="flgH"
                     /locus_tag="OO349_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HCR5865437.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(55043..55825)
                     /gene="flgG"
                     /locus_tag="OO349_000218"
     CDS             complement(55043..55825)
                     /gene="flgG"
                     /locus_tag="OO349_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HCR5865438.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYIRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(55963..56714)
                     /gene="flgF"
                     /locus_tag="OO349_000219"
                     /pseudo
     CDS             complement(55963..56714)
                     /gene="flgF"
                     /locus_tag="OO349_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
     gene            complement(56734..57942)
                     /gene="flgE"
                     /locus_tag="OO349_000220"
     CDS             complement(56734..57942)
                     /gene="flgE"
                     /locus_tag="OO349_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HCR5865439.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLLNTQGLQLTGYPVTGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     NSIAKTATTLEFNANGTLVDGAMANNIATGAINGAEPATFSLSFLNSMQQNTGANNIV
                     ATTQNGYKPGDLVSYQINDDGTVVGNNSNEQTQLLGQIVLANFANNEGLASEGDNVWS
                     ATQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYKSNAQTIKTQD
                     QILNTRVNLR"
     gene            complement(57967..58662)
                     /gene="flgD"
                     /locus_tag="OO349_000221"
     CDS             complement(57967..58662)
                     /gene="flgD"
                     /locus_tag="OO349_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HCR5865440.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGSLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(58674..59077)
                     /gene="flgC"
                     /locus_tag="OO349_000222"
                     /pseudo
     CDS             complement(58674..59077)
                     /gene="flgC"
                     /locus_tag="OO349_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
     gene            complement(59081..59497)
                     /gene="flgB"
                     /locus_tag="OO349_000223"
     CDS             complement(59081..59497)
                     /gene="flgB"
                     /locus_tag="OO349_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HCR5865441.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            59652..60311
                     /gene="flgA"
                     /locus_tag="OO349_000224"
     CDS             59652..60311
                     /gene="flgA"
                     /locus_tag="OO349_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HCR5865442.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            60387..60680
                     /gene="flgM"
                     /locus_tag="OO349_000225"
     CDS             60387..60680
                     /gene="flgM"
                     /locus_tag="OO349_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HCR5865443.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            60685..61101
                     /gene="flgN"
                     /locus_tag="OO349_000226"
     CDS             60685..61101
                     /gene="flgN"
                     /locus_tag="OO349_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HCR5865444.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTPHRGSKKISI"
     gene            complement(61141..62676)
                     /gene="murJ"
                     /locus_tag="OO349_000227"
     CDS             complement(61141..62676)
                     /gene="murJ"
                     /locus_tag="OO349_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HCR5865445.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(62843..63709)
                     /locus_tag="OO349_000228"
     CDS             complement(62843..63709)
                     /locus_tag="OO349_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR5865446.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICDSWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVNKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGE"
     gene            complement(63711..64358)
                     /locus_tag="OO349_000229"
                     /pseudo
     CDS             complement(63711..64358)
                     /locus_tag="OO349_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706988.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
     gene            complement(64369..64953)
                     /gene="rimJ"
                     /locus_tag="OO349_000230"
     CDS             complement(64369..64953)
                     /gene="rimJ"
                     /locus_tag="OO349_000230"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HCR5865447.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            65189..66397
                     /gene="mdtH"
                     /locus_tag="OO349_000231"
     CDS             65189..66397
                     /gene="mdtH"
                     /locus_tag="OO349_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HCR5865448.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSASAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            66461..67108
                     /gene="grxB"
                     /locus_tag="OO349_000232"
     CDS             66461..67108
                     /gene="grxB"
                     /locus_tag="OO349_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HCR5865449.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVANYRDNMAKQTQINLLSSMAI"
     gene            67242..67802
                     /locus_tag="OO349_000233"
     CDS             67242..67802
                     /locus_tag="OO349_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5865450.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            67908..68954
                     /gene="pyrC"
                     /locus_tag="OO349_000234"
     CDS             67908..68954
                     /gene="pyrC"
                     /locus_tag="OO349_000234"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HCR5865451.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEAVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            69028..69273
                     /gene="dinI"
                     /locus_tag="OO349_000235"
     CDS             69028..69273
                     /gene="dinI"
                     /locus_tag="OO349_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HCR5865452.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            69563..69817
                     /gene="bssS"
                     /locus_tag="OO349_000236"
     CDS             69563..69817
                     /gene="bssS"
                     /locus_tag="OO349_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HCR5865453.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            69932..71050
                     /gene="solA"
                     /locus_tag="OO349_000237"
     CDS             69932..71050
                     /gene="solA"
                     /locus_tag="OO349_000237"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HCR5865454.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVASDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDQKSDFDLTPFRLSRFQ"
     gene            71098..71211
                     /locus_tag="OO349_000238"
     CDS             71098..71211
                     /locus_tag="OO349_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HCR5865455.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            71472..72038
                     /locus_tag="OO349_000239"
     CDS             71472..72038
                     /locus_tag="OO349_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HCR5865456.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            72042..72617
                     /locus_tag="OO349_000240"
     CDS             72042..72617
                     /locus_tag="OO349_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HCR5865457.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTTKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(72659..73711)
                     /locus_tag="OO349_000241"
     CDS             complement(72659..73711)
                     /locus_tag="OO349_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HCR5865458.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            73936..74856
                     /gene="lpxL"
                     /locus_tag="OO349_000242"
     CDS             73936..74856
                     /gene="lpxL"
                     /locus_tag="OO349_000242"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HCR5865459.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPIIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            75028..75225
                     /locus_tag="OO349_000243"
     CDS             75028..75225
                     /locus_tag="OO349_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HCR5865460.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSIMGS"
     gene            75249..>75758
                     /locus_tag="OO349_000244"
     CDS             75249..>75758
                     /locus_tag="OO349_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR5865461.1"
                     /translation="MRRAISYIRFSSERQLKGDSVRRQSKLVTDWLDKNPEFYLDSSL
                     SFKDLGKSAFSGKHLKGGLGDFLTAIEKGLVKAGDTLLIESLDRLSRQDIDIASELLR
                     RILRAGVDVVTLSDGEHYTRESLKDPLALIKSILIMQRAHEESLRKSERVQAAWNRKK
                     ELISEGIKVS"
BASE COUNT        18470 a        20046 c        18742 g        18500 t
ORIGIN      
        1 gtgtttcaag ccatatgcgc aatatttcca gcatgttttc actgtgagaa agtgcaagaa
       61 atgcgcggtc tattgtttcg tggtaaatat cacgcatatt aatccccgtc cgtcgttttt
      121 cttaaaataa cctctgtcac gaaatcagca taatcggcgg cgatattcag tacatcaagc
      181 ccgttgattt atattctctc gtggaaagta agaaaaaagc cgctctaata agttctggca
      241 ttgacgaaag cgcatcagcc gcatcatcag gaacgccgga aaattcctgt ttcagggaat
      301 taaaacgatc atcacgcata acccccccca ttttcacaat cggcaacaag aatattttta
      361 gcgtcattca gtgaccgcgt tgcgatgtat cggatacatt ccagggcgaa ctgtgtccgg
      421 tctttctgtt cttctccctg cgcaagctct gccgtgcatt caatatcaat aagcgcgtgc
      481 atcagcgtag tgagtgcggc ggctgctgcg ttcggtgtgg ttttattgtg cataggtttc
      541 cccctgacga atacgggcgg cgaatacagc aacacaaccg gacgggcaac ggctacgcgc
      601 ttcgcgttcc gtccaagcgg ttacgtggat gatttgggat tctccggcac tcagtgccag
      661 aaaacgccac acaaaggccg tttgtgtgtg tacaaggtgt ggtatatgat ttacggcaac
      721 cataacggct cctagtttac gttgttggtt agaagccccg ttactgctcc tacagtgcgg
      781 ggctttgctt tttgtgttga ttgtgtgtaa tgtgtcacta cacataaata cattacctga
      841 ggtgtaatta acgtgtcaac acacaaaaac gaaaggcgtg gtaatcctcc gttccagttc
      901 cgtttagatc ctgacttacg ggaaaagatg gaaaaggcac aacaacagga cggcgatgat
      961 tctcttgctg cctggataaa gcgaataatc cgcaaagaat tacagcaacg cggcatagaa
     1021 cccaaaggat aaaaactatc agcgccgtgg tgtgaggtac tacggcgcat tgctttacag
     1081 gcgcacacaa tgaccaacga agaatcaacc gaaaaaacat cacctgcacg aaaaagacga
     1141 cgcagaaaga aagagcatga atcagaacga ttcgcgccgt gttcttttgc ccttgagaag
     1201 taccttaaag aatactcatc aaaggagaga acttcgcagt tatggcaacg cactgaacca
     1261 gactgattgc attgcccacc agcctgatag cggctatcat ccccgtgctt atgtttggga
     1321 tcacacacac aaacggcgca gcgggttatc tgttcagaaa ggcggctcca tttcggggcc
     1381 gcttttttta tgcctgaaaa accccaattt tgtggttttc cagtttcacc agggcgaacg
     1441 aatccccgcc cacgttctgg cgtatattca atcttcatgg ttatagctct gtgttcagat
     1501 gattgatgtg tggcggctgc gtgccgccag cgtgattaat gaactgcctt gcagctatcc
     1561 ttccaggcca gaacctcgga taaagaccag ccaacggaac gaccgccaag tttacgacgt
     1621 gatgggaatt gtccggcctt ttccaggcgg tagcggcatg agcggctaag gcctgttagc
     1681 ttttcgcatt ctttttcacg tataaaccga tcagtgctta acactattgc cccctttcgt
     1741 ttcttaaaga gttatttcgt gttctattgc gttgggatgt gtctgattgt gtcaggatga
     1801 ttcagagttg gcaaatgttg aggccgcatg gtttacagaa agaggaataa tcaggataaa
     1861 atcctttaaa ttcatgttaa tacaaaggca taaaatattg tttcatgcct tttttctcgc
     1921 tatttaaaga gtgattcgct agtgtataaa aaatcagcaa cacattaaaa atcagctatt
     1981 tataaatctg tacacttttt cacctcttgt tcgtagttgt tccgctttgt tgctcattgt
     2041 tgcacgttgt atctgttcgc atatccagta tgcgcatact gaaaaaacac gaaaaaaatt
     2101 attttcttct ggctactggt agcgtggtta cgttttcatg tgttcccgcc agtatcccta
     2161 accgctccgt ccacatatcc agcgcattgc gtttagcatc cagataacgg gaatgattat
     2221 aaactcgctg cattcctggc atctggtggc ctgtaagctg ctccacgacg tgaggatcca
     2281 cgcctaaatc gttcagcatc gttgtaaagg tgcgccggat gtcatgtagt gaccagtgag
     2341 ggtgatttag cctcctgtgc gctaatctgc cgtactgcga cacgcttgtt tcctgtttca
     2401 cttcccccag caataagccc gtgtgcctgt tctgctccac cagctgcgtg acgaacggca
     2461 gtattgcttc cggtatgggc cggaatattg ccaccttcgt tttgctgtgt tctttcggca
     2521 cggtccagag catttcggta aaatcccact cgctgatctc cgataacctc agttctaccg
     2581 tccggcatcc gaacacaatc aggaggcgga ttaacgcgat gtagtagggg gagaatattt
     2641 ttttgtccag tgcctgcaat aattcgccca gttctttggt gcttaagaca cgctcgctta
     2701 tatccggttt tttcccaacg tccgccacac tcatatcatc aagaacgttg ctgattgcat
     2761 agcgccgcct ccggcagaac ttaagcgcct gtttgcacgt ctgtagcaag aatccggcag
     2821 taacaggcgt tcgctttgcc acctggtcaa aacaggccag ccagtgccgt agctcgcatt
     2881 tatccagcgg catagcacca atgtgctgta ttacgtgatt attaaggcgc tttttcaggg
     2941 cggcataatc cacgcggttt tcctttgcgt acgactcaag ccagtaggtg agcgcatcgc
     3001 caaccgttac cggctttaac gcttcctgta cggtgtaatt aagctcatga cgtggatttt
     3061 cccctctgcc agccatgcgc gacactgggc ggctttttcc ctggctgatt tcaggttcag
     3121 atcaggataa tttcccagct taatgcgttc cggtggtgcc ccccttcccg ttccggccct
     3181 gtaagtgaaa taccaggtta aaaggccact ggttgaatgc ctgacgctca ggtttccacc
     3241 gtcattaaga aaggctgttt tttgggcggg tgtgccgttg attttcctct gctgtgtatc
     3301 gctcagtttg ttaagtgctc tgctcataat tttgattccg gtcatcacaa ttaactacac
     3361 cattaactac accgatcgtt gcacaaaggg ctacaacgtg aaacaagctg gaacaaggaa
     3421 aatcacaaat ctgtttataa tcaaaaacat aatgaacaat ctgaaacatt atgaaacggc
     3481 aaaaaacact aattgataaa atatgtccat acttcacgca ttacgttaag catccgttat
     3541 aatcggttgc agataccagc ctgtggatgc ttaacatgga ataccaactc actcttaact
     3601 ggcccgattt tcttgaacgt cactggcaga aacgcccggt ggtgttaaaa cgcggcttta
     3661 ataattttat tgacccgatc tctccagacg agttggcggg tctggcgatg gaaagcgaag
     3721 ttgacagtcg actggtcagt caccaggatg gcaaatggca ggtcagccac ggcccgttcg
     3781 aaagctacga tcatctcggt gaaaccaact ggtcattact ggtacaggca gtaaaccact
     3841 ggcatgagcc gaccgccgcg ctgatgcgac cgttccgtga actaccggac tggcgtattg
     3901 atgatctgat gatttctttt tctgtacctg gcggcggcgt cggcccgcat ctcgatcagt
     3961 acgacgtgtt tatcattcag ggtaccggac gtcgtcgctg gcgagtgggc gaaaagctgc
     4021 aaatgaaaca gcactgccca catccggatc tgttacaggt cgatccgttc gaagccatca
     4081 tcgatgaaga gctggagcct ggtgatattc tttatattcc gccaggattc ccgcatgaag
     4141 gctacgcgct ggaaaatgcg atgaactatt ccgtgggttt tcgcgcgcca aatacgcggg
     4201 aattaattag cggatttgcc gattatgtgc tgcaacgtga actgggcggc aactactaca
     4261 gcgatccgga tgttccacct cgtgctcatc ctgcggatgt tctgccgcaa gagatggata
     4321 aactgcgtga gatgatgctc gaattgatca accagccgga acactttaag caatggtttg
     4381 gcgagtttat atcccagtca cgtcatgaac tggatatcgc gccgccggaa ccgccttatc
     4441 agccagatga aatctacgat gcgctgaaac aaggtgatgt gctggtgcgc ctgggtggtc
     4501 tgcgcgtatt gcgcattggc gacgacgtgt atgccaatgg tgagaagatc gattccccgc
     4561 accgtccggc actggatgca ctcgccagca acattgcgct gactgcggag aattttggcg
     4621 atgcgctgga agatccgtca ttcctcgcga tgctcgcggc gctggtcaat agcgggtatt
     4681 ggttcttcga agggtaagtt tggatttaag ccggatgcag catcacatgc cgcatccctt
     4741 ttcgcttact tccgttgcgc cgttaactcg gcaatacgga cgatcacctg caccgctttt
     4801 tccatacctt ccagaatcac aaactcatgc ttaccatgat agttgtaacc gccagtgaac
     4861 aggttcgggc aaggtaatcc cataaacgac aactgcgcgc cgtcggtacc gccgcggatc
     4921 ggtttcagtt ccggttcaat atcgcagtca cgcatcgcct gctgggcgat atcgagaata
     4981 tgcggatgct caaccacttt ctcgcgcata ttgtagtaac tgtcttcaat caccaattca
     5041 atgtagcaat caggatgtaa ccctttgccc acttttttgg cgatctccat cattttacgt
     5101 ttacgcgctt caaactgttt acggtcgaaa tcacggatga tgtagtgcat atcggcccgt
     5161 tcaacggtgc ctttcatgct cgccaggtga tagaaacctt catagccttc tgtcatttcc
     5221 gggctttcat ccgccggaac ttccgcatga atacgtgccg ccagcgacag cgcatttacc
     5281 atcactcctt tcgccgtgcc cggatgaaca ttgttaccga caatttttat attgaccgat
     5341 gcggcgttga agttttcaaa ctccagttcg cctacgccac caccgtcaac agtgtaagcc
     5401 cagcgggcat cgaaggcatc aacatcaaaa tgtttcgccc ctttgcccac ttcttcatcc
     5461 ggggtaaagg cgacgcgaat atcaccatgc ggaatgtttt tctgttgcaa taccgccagc
     5521 gcggtcatga tttctgcaat acctgctttg tcatcagcac ctaacaaggt tttaccatcg
     5581 gtggtaatca acgtctgacc cagtagctga tgcagcaccg ggaacataac cggtgataaa
     5641 acttcatcgc cgatacccag cgcaatatcg ccaccgcgat agttttcaac aatttgcgga
     5701 ttaacatttt tgccgctgca atccggtgag gtatccacat gagaaataaa gccaatcgcc
     5761 gggatatcgc cagggacgtt agccggtaac gtcgccatca aagtgccctt ctcacttaag
     5821 gtcacattga taagccccat ttcttcgagc tgctctttca gcagatgcaa taacttccat
     5881 tggccttccg tgctgggaac ctgtctcacc cctgcttttg attgggtatc cagagacacg
     5941 tagttcaaaa atcgctcaag tagtttatcc atgtagtcac cctcactttt tgtgacaaca
     6001 ttattaagaa gcaagaaaag acaaatattg cgtcaggtca cttttacccc tgcaagcggg
     6061 aatatttatc agcattacct taatgaatat aaagctaagc cagtaattca caacaaagat
     6121 tggcgattca agcggtttgc cgtagaatta ccgccctcat taagagtcac caaggtggtt
     6181 aaccacaaac cccgcatcgg taagccatcc gttgcgttta catgggacag agtaaaaaat
     6241 tgaataaaca accgagttcg ctttcaccgc tggtgcaatt ggcgggaatt cgcaaatgct
     6301 ttgatggtaa agaggtcatt ccccagctgg atctgactat caacaatggc gagttcctca
     6361 cgctgcttgg cccttctggc tgcggtaaaa caaccgttct tcgcctgatt gcaggtctgg
     6421 aaactgttga ttccggacgc atcatgctgg ataacgagga catcacccac gttccggcgg
     6481 aaaaccgcta tgtgaacact gttttccaaa gctatgcact tttcccccac atgaccgtgt
     6541 tcgaaaatgt ggcctttggg ttgcgcatgc aaaaaacccc cgctgctgaa attacgcccc
     6601 gcgtgatgga agccctgcgg atggtgcagc tggaaacctt cgctcaacgt aaaccgcatc
     6661 agctctctgg tggtcaacag caacgcgtcg ccattgctcg cgcggtggtt aacaagcctc
     6721 gtctgttgtt gctggatgag tcgctctcag cgctggatta caaactgcgt aagcaaatgc
     6781 agaacgagct gaaagcgtta cagcgtaagc ttggcattac attcgttttc gtgactcacg
     6841 accaggaaga agcactcacc atgtcggaca ggattgtggt gatgcgcgat ggtcgcattg
     6901 agcaagacgg cacgccgcgt gaaatctacg aagagccgaa aaacctgttt gttgccgggt
     6961 tcattggcga aattaatatg tttaacgcca cggtgatcga gcgactcgac gagcagcgcg
     7021 tacgcgccaa cgttgaaggc cgcgaatgta atatctacgt taacttcgcc gttgaaccgg
     7081 ggcaaaaact gcatgttctg ctgcgcccgg aagacttacg tgttgaagag attaacgacg
     7141 acaaccacgc tgaagggctg attggttacg ttcgtgagcg taactacaaa ggcatgacgc
     7201 tggagtcggt tgttgaactg gaaaatggca agatggtgat ggtcagcgaa ttcttcaatg
     7261 aagacgatcc tgactttgac cactctctcg accaaaaaat ggccattaat tgggtagaaa
     7321 gctgggaggt cgtactggct gatgaagaac acaagtaagt tccagaatgt agtgattgtc
     7381 actattgtcg gttggcttgt gttgtttgtc tttctgccca acctgatgat cattggcacc
     7441 agctttttga cccgcgacga cgccagtttc gtcaaaatgg tctttacgct ggataactac
     7501 acgcgtctgc tcgatccgct ctattttgaa gtgctattgc actcgctgaa tatggcgctg
     7561 atcgccaccc tcgcctgcct ggtgctgggc tatccatttg cctggtttct ggcgaagctg
     7621 ccacacaagg tgcgtccgct gctgctgttt ctgctgattg ttccgttctg gaccaactca
     7681 ttgattcgta tctacgggct gaaaattttc ctcagcacca aaggctatct caacgagttt
     7741 ttgctctggc tgggcgttat cgacacacca atccgtatca tgttcacgcc cagtgcggtg
     7801 attatcggtc tggtttacat tctgctgccg tttatggtga tgccgctgta ctccagtatc
     7861 gaaaaactgg ataagccgtt gcttgaggcg gcgcgcgatc tcggtgccag caagttacag
     7921 acttttatcc gcatcattat tccactgacg atgccgggga ttattgccgg atgtctgctg
     7981 gtgatgctgc cagcgatggg cctgttctat gtgtccgacc tgatgggcgg tgcgaaaaac
     8041 ctgctgatcg gtaacgtcat caaggtacag ttcctcaata ttcgtgactg gccgtttggt
     8101 gcagccacca gcattacgct gactatcgta atgggcctga tgttgctggt ttactggcgc
     8161 gcttctcgtt tgctgaataa gaaggtggaa ctcgaatgat cggtcgactg cttcgcggcg
     8221 gttttatgac cgctatctac gcgtacctgt atatcccaat cattattttg attgtgaact
     8281 cctttaacag ctcgcgcttt ggcatcaact ggcagggttt taccaccaaa tggtatagcc
     8341 tgctgatgaa caacgacagc ctgttacagg cagcgcagca ttcactaaca atggcggtgt
     8401 tttcggcgac gtttgctacg cttatcggtt cactgacggc agttgcgctg taccgttatc
     8461 gctttcgcgg taagccgttc gttagcggaa tgctgtttgt ggtaatgatg tcaccagata
     8521 tcgtgatggc aatttcgctg ctggtgctgt ttatgctgct gggtattcag cttggcttct
     8581 ggtcgctgct gttctcgcat atcaccttct gcctgccatt tgtggtggtg acggtgtatt
     8641 cgcgcctgaa aggttttgac gtgcggatgc tggaagcggc gaaagatctc ggtgccagcg
     8701 aatttaccat tctgcgaaaa atcattctgc cactggcaat gccagcggtg gcggcgggct
     8761 gggtgttaag ctttaccctg tcgatggacg atgtggtagt ttcttcattc gtcaccggac
     8821 caagttatga aattctgccg ttaaaaattt attcgatggt caaagtcggc gtatcgccgg
     8881 aagttaacgc gctggcaacc atattactgg tgctgtcgct ggtgatggtt attgccagcc
     8941 agcttattgc tcgtgataaa acgaaaggta acacagggga cgttaaatga aaaaatggtc
     9001 acgccacctg ctcgcggcgg gtgctctggc actgggcatg agcgccgctc acgccgatga
     9061 caacaacacg ctgtatttct acaactggac cgagtacgtg ccgccaggac tgcttgaaca
     9121 gttcaccaaa gaaaccggta ttaaggttat ctattcgact tacgagtcga acgaaaccat
     9181 gtacgccaag ctgaaaacgt acaaagacgg tgcctatgac ctggtggttc cttcaaccta
     9241 ttacgtcgat aaaatgcgta aagaggggat gatccagaag atcgacaagt cgaagttaac
     9301 gaacttcagc aatctcgatc cagacatgct caacaagcct tttgacccga ataacgacta
     9361 ctccattccg tatatctggg gtgcgacggc gattggtgtt aacggtgatg cggtggatcc
     9421 gaaatctgtc accagctggg ccgatctgtg gaagccagag tacaaaggca gcctgctgtt
     9481 gaccgacgat gcccgtgaag tgttccagat ggcgctgcgt aagctgggct actccggtaa
     9541 caccaccgat ccgaaagaga ttgaagctgc atataacgag ctgaaaaaac tgatgccaaa
     9601 cgtcgcagcg tttaactccg ataacccggc taacccgtac atggaaggcg aagttaacct
     9661 cggcatgatc tggaacggtt ctgctttcgt tgcacgccag gcgggtacgc ctattgacgt
     9721 ggtgtggccg aaagaaggcg gcatattctg gatggacagc ctggcgatcc cggcaaatgc
     9781 caaaaacaaa gaaggcgcgc tgaaattgat caacttcctg ctgcgcccgg atgtggcaaa
     9841 acaggttgct gaaactatcg gttatccaac gccaaacctt gcggcgcgta agctgttaag
     9901 tccagaagtg gcaaacgata agacgctcta cccggatgct gaaaccatta aaaatggcga
     9961 atggcagaat gacgttggcg cagccagcag catttatgaa gagtattatc agaagctgaa
    10021 agcaggacgt taattcagca agcgggcggt ggtattaccg cccgtttttt agttagcaag
    10081 gatgtggcta atgagtcagg acagtaaagt tttatcccgc tctttcgtcg gtattgtggt
    10141 ccgcttgtag gcctgataag cgtagcgcat caggcaatgc ttcctgcttt ataatccctc
    10201 cagcaacttt tcaacaaatt ctggcaccac ctggcttgcc gggccgtaat atttctcggc
    10261 aaattcatta ccaacctgac tcggttcaag attcagttca acggtgtgag caccatgtag
    10321 tttggcttcg tgtacaaacc cagccgccgg atagacatgc ccggaagtac caatggcaat
    10381 aaagacatcc gccatcgaca acgccatata aatttcatcc atgccgagtg gcatttcgcc
    10441 aaaccaaacg acgtgcgggc gcaagggtgc cgggaactgg caacaatggc atttatcttc
    10501 tggcgtaacg tctccggtcc agtcgagaac ctgaccactt tgcgaacaac gaactttcag
    10561 caattcccca tgcatatgaa tgacattggt attacctgcg cgttcatgca ggttgtctat
    10621 attctgcgtt accagcaaaa agcgatcgcc gagagcgtct tgcagtttag ccagcgcaag
    10681 atgcgcggcg ttaggctgaa tttctggctg ctgcagctgt cgacgacggg cgttataaaa
    10741 cgcttgcacc agttcaggat cgcgatcgaa accttccggc gttgccacat cttcaacccg
    10801 atgttcttcc cacaggccat ctgcggcgcg aaaggtacga atacctgatt ccgcagaaat
    10861 tcctgcccct gtcagtacga gtactcttgg tttttccatt gcttccggca ccactttatc
    10921 tctgaaaaaa atacgctgac gcaaacgctc gcgcaggcga cgtttatttt tacgaaaacg
    10981 acttaaccga tgaccccgac gcgacagcat agcaacctct gttgtttaat cggttagatg
    11041 taggaaggcc gcaccacgca ttcctcccgc atcaccgtgg cgcgcgcgtt caatgcgcgg
    11101 aacacgagct acaggtaaga gatgacaagg caacctgtcc gccagttgcg ttgtgattgc
    11161 cgggaaattc gataagccac caccaatgac gaccaggtca gggtcaacaa tggtcaggat
    11221 atttcccaga gaaaccgcta ataaatccag ataacgctca acgtgcgccc ttgcctgctc
    11281 atcgccttga tcataaagcg caataatttc gggagcctgc aacggttgat gataatagtg
    11341 ttgatacagc cacgcaaaac cgcgaccaga cagataattt tcaatgcagc catgctgacc
    11401 acagccgcag cggcgtaacg ggaaatccaa ccccatcatg gttaacgcat caaccggcag
    11461 acgcatatgg ccaaactcgc cggtaatgta gcttttacca gtaatcggtt tgccgttgaa
    11521 aaccagcccg ccgccaacgc cggtgccgag aattaacccc atcaccagtg gatattgcgt
    11581 aaattcatca tcccaggctt ctgaaagggc aaaatagttg gcatcgttat caaggcgtac
    11641 atcgcgatca agacgtgcgc tcaggtcggc acgcagcggt ttgccgctgg cagcggggac
    11701 attagcggca tacagcttac catcttctgt ttccggcatt cccggaatac cgatgccgac
    11761 agaaccttta cagccaaaac gttggtcagc ttcagctacc agctcacaca ctgcatctaa
    11821 aaatgcgtca tagctgtcac gcggtgtcgg cacccgcttt tcccactgca actgccgacc
    11881 gctatcaaac acgccaagcg caatttttgt tccaccaata tcaaacccgt aatacattgc
    11941 cgctccttgt tttaatgtac tgcctttact ggccgctaag gactcgcgca gggtcaatat
    12001 tactggcgcg ccgcgccgga taccaacttg ccaaaagact cagcaacaat gctgtgacca
    12061 gtacgtagaa gacgtccagc cagtgcaatt ccgatggcag gaagtcaata aaataaatat
    12121 cgctggagag gaactgatga ccgatcagct tttcaatcca ctcaataatc ggggtaagtt
    12181 gcagtgaaac aactacgccg ataaccaccc cacacaggct gccgaacagc cctgccagca
    12241 atccatacca gacaaagatg gcgcgaatta aaccatcttt cgcccccagc gttcttaata
    12301 ctgcgatatc gccactcttg tctttcaccg ccatcactaa ggtggagacg atgttgaaac
    12361 aggccacgcc aatcaccagt accatcgcca gatacataat ggcgcggatc atctggatat
    12421 cgcgatacat atagccgtaa gtaccaatcc aacttttaat ataaacatag ctgttggtca
    12481 cttcacccgc atcgcgtacc agcttattgg cgttgaaaac atccgtcatt ttaagggcaa
    12541 tacctgacac gctggaaccc atatcaagat attgttgggc atccgccagc gggatcatgg
    12601 caaaactgtg atcgagttga ccactcaact gcaaaatacc ggcaatgtgc aaacgcacac
    12661 gttttggctg catcagttta tgctcgggat tcgagttggg gatcatgatc gacacccaat
    12721 cgccctgctt cactttcagc gcatccgcca cgcctttgcc gatgataatt tgctgttcgc
    12781 ccgctttaaa attgcgccac gcatccccct gaacaaacga gggtaatgcg ctcagacgct
    12841 gttcctgttg tgggttaacg cccttcacct ggattgcgcg cagattcgct ccactttcca
    12901 ccagaccggt gaaattgata tacggcgcag cggcggcaat acccggcact ttctgcacgt
    12961 tatccagtgc ttcctgccag ttagtccatg gctgatttac cgcttcgatt tcgccatgcg
    13021 gcaccaccgc cagaatgcgg ttattcagtt cgcgttcaaa gccgttcatc gcgcttaagc
    13081 cgacgatcaa taccgccacg ccgagggcaa tgccaatggt agaaatgaca gagattagcg
    13141 acaccatgcc gccgcgtcgc cgtccgcgac taaaacgcag gccaatcaat aacgataaag
    13201 gcatcgccat tactccgccc ccatcaggct cagttccgcc gtcagacgtc catcacgcat
    13261 ttccagttgg cggctcatac gtttcgccag ttgcaggtcg tgagtgacca ccaggaaagc
    13321 ggtgccctgc aagcgattca actccccaag caactggaag atgctgtcgg cgttacgcgc
    13381 atcgaggtta ccggtaggtt catccgccag taccagacgc ggattattga ccagcgcacg
    13441 ggcaatcgct acacgctggc gttcgccgcc agaaagttca gatgggcggt gattcgcacg
    13501 atgctccagc cccaccgctt ttaacatctc cagtgcacag ctgttgattt cagcgggctt
    13561 tttcttgcca atcagcagtg gcatagccac gttttccagg gcagtaaaat ccggcagcag
    13621 gtggtgaaac tgataaataa agcccagctt ctggttgcgc agttctgctt tcgccgctga
    13681 agacagtttg ctcatcggct gaccgttaaa gatcacatcg ccggaggttg gcgtatccag
    13741 cccgcctaac aggtgcagca aggtactttt accggaacca gagctaccga caattgccat
    13801 catttcacct tcgcccacgc tgaaactgac attgagcagc acatcggttt gcacactgcc
    13861 ttcctgatag cgtttgcaca ggttgtcgca ttgcaacagg atcttattca taacgtaaag
    13921 cctcagcggg ttgtgtggcg gcagcgcgcc atgaagggta aagcgtagac agcagcgcga
    13981 tagccatcgc caccagcgca ataacaatga cctgtaaagg ttcgatagcc accggcagcg
    14041 ccgcgccatc aagcaggacg ccgattatcg gcatcagatt atttaactgg ctggcaagca
    14101 gtgcgccaag cgccgctccg aggattgcac cgataatccc ggcgctagcc ccttgcacca
    14161 taaagaccat catgatttgt cgcggagtta agccttgcgt ttgcaggatc gcgacttcgc
    14221 cctgcttctc catcaccatc agccccagcg aggtaataat gttaaacgcc gcaacagcga
    14281 caatcaggct cagcagtaag cccatcatat ttttttccat gcgtacggcc tggaacagct
    14341 cgcctttacg gtcacgccag tcctgccatt tgctgccttc aggcagtttt tgctgactta
    14401 aagagtcaac tctcagcggc tcatccagcc acaaacgcca gccggtaata ttggctgccg
    14461 gataacgcat cagacgcgat gcatcctcaa tattcaccag catttcatag ccatcgactt
    14521 cactgttggc ggcgaaagtg ccaatcacat tgaacaggcg ctgacttgga atacgcccca
    14581 tcggcgtgaa ctggctggca gatggcacca tcacgcggat ttgatcaccg cgattgacgc
    14641 ccaactgtga ggcaagttgt tcgccgagga tgacattata tttccccggc tcgaggtcag
    14701 tttgtttcac attgaccaga tacggcgtta gtggatcttt ttgcgccggg tcgataccga
    14761 gcatcacccc gaccgccacg ctgcgcgcgc tttgcagtac cacatcaccg gtagtaatag
    14821 gtgcgacgcg attaacgccg tccagtttga ctgccgtttc cgggagttgc tgcgggttaa
    14881 gagagccatg ctcagaagag agaattgcct gtggcatcag gccaaggatg ttgttttgca
    14941 gctcgcgctc aaagccgttc atcactgaca atactgtgac cagcgccatc accccgaggg
    15001 taatgccgat ggtagaaagc caggagacga aacgaccgaa gcgatccact gcacgcccac
    15061 gcatgtaacg caggccaata aatagagcga caggttggta catgaaatcc gtctggttgc
    15121 tgtagcaaag acacgagtat ataagcgaaa gcaagatggg taaacgtctg aactgtaagt
    15181 gatcgtcagg gctaatctga aaagcccccg taaatcaatc ggctaaattg ccatgtttta
    15241 tcatcaatct tgccgcctga tcttaatctg aaaaatcatc caatacagac agatacccat
    15301 tacacctcgg gtcataacca gcaggatcaa ccgcatgtaa attaaaaaag agcgtgttgt
    15361 tctgatgaaa caaaaagagc tatggattaa ccagatcaaa gggttatgta tttgtctggt
    15421 ggtgatttat cactcggtca ttacctttta tccgcatatg accacttttc agcatccgtt
    15481 atcggaagtc ctgagcaaat gctggatcta tttcaatctt taccttgccc cctttcgtat
    15541 gccggttttt ttctttatat ccggctattt gattcgccgc tatatcgaca gcgtaccgtg
    15601 gggaaattgt ctcgataaac ggatctggaa catcttctgg gtgctggcgc tttggggcgt
    15661 ggtgcagtgg ctggcgttaa gtgcgctgaa ccagtggctg gcacctgagc gcgatttaag
    15721 caatgcctcc aatgccgctt atgccgattc caccggtgag ttcctgcacg ggatgatcac
    15781 cgccagcacc agcttgtggt atctgtatgc gttaattgtc tatttcgtgg tatgtaaaat
    15841 ttttagccgt ctggcgctgc cactattcgc cttgtttgta ctgctgagtg tggcggttaa
    15901 tttcgttccc acgccgtggt ggggaatgaa cagtgtgata cgcaatttgc cttattacag
    15961 ccttggcgca tggtttggcg caacaataat gacctgtgtt aaagaggtgc cgttgcgccg
    16021 ccatctgctg atggcttctt tgcttaccgt tctggcggtc ggtgcctggt tgtttactat
    16081 ctcgctgctg ttgtcgctgg tatcgattgt ggtgatcatg aagctgtttt atcagtacga
    16141 gcaacgattc ggtatgcgct ccaccagcct gctgaatgtg attggctcca acaccattgc
    16201 tatctacacc acccatcgca ttctggttga aatattcagc ttaactctgc ttgcgcaaat
    16261 gaacgcagca cgctggtcgc cgcaagtcga actgacactc ctgctggttt atccctttgt
    16321 cagtttgttc atctgtactg ttgcgggctt gctggtaaga aaactttcac agcgtgcatt
    16381 cagcgatctg ttgttctccc cgccttcact gcccgctgcc gtcagttact ctcgttaaac
    16441 catcacccca gccggggcga ttcaatttgc taaaccatgt cgattacgga taatgaacag
    16501 cattgcttat cagagatatg cccccatatg ttgaggcata tcctaacgag aatctgacaa
    16561 ccgttatgcc tgaacaatat cgttatacgc tgcccgtcaa agcgggtgag cagcgtctgc
    16621 tgggcgagtt aaccggcgca gcctgtgcaa cgctggtagc ggaaattgcc gaacgtcacg
    16681 ccggtccggt ggtactcatt gcaccagata tgcaaaatgc tctgcgtttg catgatgaaa
    16741 tcagccagtt caccgatcag atggtgatga atctggcgga ctgggaaact cttccctacg
    16801 acagtttttc gcctcatcag gacattatct cctcgcgcct ttccaccctt taccagctac
    16861 cgacgatgca gcgtggcata ctgattgttc cggtgaatac gcttatgcag cgcgtttgcc
    16921 cacacagttt tctccacggt catgcgctgg tgatgaaaaa aggtcagcgc ctgtcacgag
    16981 atgcattacg aacccaactg gacagcgccg gttatcgcca tgttgaccag gtgatggagc
    17041 acggcgaata cgccacgcgc ggcgcgttgc tggatctctt cccgatgggg agtgagctgc
    17101 cttatcgtct tgatttcttt gatgatgaaa tcgacagcct gcgggtgttt gacgtcgaca
    17161 gccagcgcac gctggaggaa gtagaagcga tcaatctgct gcccgcgcac gaatttccga
    17221 ccgataaagc ggcaattgaa ctgttccgca gccagtggcg cgataccttc gaagtgaagc
    17281 gcgatccgga acatatttac cagcaagtga gtaaaggcac attacctgct gggatcgagt
    17341 actggcagcc attgttcttc agcgaaccac tgccgccgct gttcagttat ttccctgcca
    17401 ataccttgct cgtgaatact ggcgatctgg aaaccagtgc cgaacgtttc caagctgaca
    17461 cgctggcgcg ttttgagaat cgcggcgtcg atccgatgcg cccgctgttg ccaccacaat
    17521 cgctctggct gcgggtggac gagctcttct cagagctgaa aaactggccc cgagtgcagc
    17581 taaaaactga acatttaccg acaaaagccg cgaatgccaa tttaggtttc cagaaactgc
    17641 cagacctggc cgttcaggcg caacaaaaag cgccgctgga tgcgctgcgt aagttcctcg
    17701 agactttcga cggtccggtg gtgttctcgg tagaaagtga aggtcgccgt gaagcgctgg
    17761 gtgaactgct cgcacgaatt aaaattgctc cgcaacgcat tatgcgtctt gatgaagcca
    17821 gcgaccgtgg gcgttatctg atgattggcg ctgccgaaca tggttttgtc gataaggcgc
    17881 gtaatctggc gctgatctgc gaaagcgatc tgctcggtga acgtgttgcc cgccgtcgtc
    17941 aggattctcg ccgcaccatc aaccccgata cactgatccg taaccttgcg gagctgcata
    18001 ttggtcagcc ggtggtccat ctggagcacg gcgtcggtcg ctatgccgga atgaccacgc
    18061 tggaagctgg cggcattact ggcgagtacc tgatgctcac ctatgccaac gacgccaaac
    18121 tgtatgttcc ggtgtcgtca ctgcatctga ttagccgtta cgcaggtggc gcggaagaaa
    18181 acgccccgct gcataaactt ggcggcgatg cgtggtcacg cgcgcggcag aaagcggcgg
    18241 aaaaagtgcg tgatgtggcg gcggaattgc tggatatcta cgcgcaacgc gccgccaaag
    18301 agggcttcgc gtttaaacac gatcgtgagc agtatcagtt attctgcgac agcttcccgt
    18361 ttgaaaccac gccggatcag gcgcaggcca ttaatgcggt acttagcgac atgtgtcagc
    18421 cgctggcaat ggatcgtctg gtgtgcggcg atgttggctt tggtaaaaca gaagtggcga
    18481 tgcgcgccgc tttcctggca gtagataacc acaagcaggt agcggtgttg gtgcctacca
    18541 cccttcttgc gcagcagcat tacgacaact tccgcgaccg tttcgccaac tggccggtac
    18601 gtatcgaaat gatctcccgt ttccgcagcg ccaaagagca gacgcaaatc cttgcggaag
    18661 tggcggaagg gaaaatcgat attctgatcg gtacgcacaa actgctgcaa agtgacgtca
    18721 agtttaaaga tttaggcctg ctgattgtcg atgaagaaca ccgcttcggg gtgcgtcata
    18781 aagagcgcat taaagcgatg cgcgcgaacg tggatattct gacgcttact gcaacgccga
    18841 tcccacgtac gctgaatatg gcaatgagcg gaatgcgtga cctgtcgatt atcgccacgc
    18901 cgcccgcccg tcgtctggca gttaaaacct ttgtccgtga gtatgacagc ctggtggtcc
    18961 gggaggcgat cctgcgtgaa attttgcgcg gaggacaggt ttattatctc tacaatgatg
    19021 tggaaaacat tcagaaagcc gccgaacggc tggcagaact ggtgcctgaa gcacggattg
    19081 ccatcggtca cgggcagatg cgcgagcgcg aactggaacg ggtgatgaat gatttccatc
    19141 atcaacgttt caacgtgctg gtttgtacca ccattatcga aaccgggatc gacatcccga
    19201 cagccaacac cattatcatt gaacgtgcgg atcacttcgg tctggcgcag ctgcaccagt
    19261 tacgcggtcg cgtcggacgt tcacatcatc aggcatatgc atggctgctg acgccgcatc
    19321 caaaagcgat gactaccgat gcacaaaaac gtcttgaagc gattgcctcg ctggaagatc
    19381 tcggggcagg ttttgcgctg gcaacgcacg atctggagat tcgcggcgcg ggtgaactgc
    19441 ttggcgaaga acaaagcggt tcaatggaaa ccatcggttt ctcgctgtat atggagttgc
    19501 tggaaaacgc cgtcgatgca ctgaaagccg gacgcgagcc gtcgctggaa gatctcacca
    19561 gccagcaaac agaagtcgag ctgcggatgc cgtcgctatt gccagatgat ttcatccctg
    19621 acgtgaacac gcgtctgtcg ttctacaaac gtattgccag cgccaaaacg gaaaacgaac
    19681 tggaagagat caaagtcgag cttatcgatc gcttcggcct gctgccagat ccggcgcgta
    19741 ccctgctgga tattgcccgt ctgcgccagc aagcgcagaa actggggatc aggaagctgg
    19801 aaggtaatga gaaaggcggg gtgatcgaat ttgccgagaa gaatcacgtt aatccggcct
    19861 ggttgattgg tttgctgcaa aaacagccgc agcattaccg ccttgatggt ccgacgcgcc
    19921 tgaaatttat tcaggatttg agtgagcgga aaacgcgtat cgaatgggta cgccagttta
    19981 tgcgtgaact ggaagagaac gcgatcgctt aatgtaaatc gggggcgttt ctgcgccccc
    20041 atctgcaaca tttacaaatt ctttgcactt tcctgcacta tccgacactg tcaccatcca
    20101 taattcaggc ttatctgttt attacaataa ccttatattt attatgtatt tttggtgatg
    20161 atcaaaacgc gtttttctcg ctggctaacg ttttttacgt tcgccgctgc cgtggcgctg
    20221 gcgctaccgg caaaagccaa cacctggccg ctgccgccag cgagcagtcg tctggttggc
    20281 gaaaacaaat ttcatgtggt ggaaaatgac ggtggttctc tggaagccat cgccaaaaaa
    20341 tataacgtcg gctttctcgc tctgttacaa gctaaccccg gcgttgatcc ttacgtaccg
    20401 cgcgcgggca gcgtgttaac gatcccgttg caaaccctac ttccagacgc gccgcgcgaa
    20461 ggcattgtga tcaacattgc ggagctgcgt ctctattact acccgccggg taaaaattcg
    20521 gtaaccgtgt atccaatagg tattggtcag ttaggtggtg acacgctgac accgacaatg
    20581 gtgaccaccg tttcagacaa acgtgcaaac ccaacctgga cgccaacggc aaacatccgc
    20641 gcccgttata aagcacaggg aattgagttg cctgcggtag tgccggctgg accggataac
    20701 ccaatgggcc atcatgcgat tcgtctggcg gcctatggcg gcgtttattt gcttcacggt
    20761 acgaacgccg atttcggcat tggcatgcgg gtaagttctg gctgtattcg tctgcgggat
    20821 gacgatatca aaacactctt tagccaggtc accccaggca ccaaagtgaa tatcatcaac
    20881 actccgataa aagtctctgc cgaaccaaac ggtgcgcgtc tggttgaagt acatcagccg
    20941 ctgtcagaga agattgatga cgatccgcag ctgctgccaa ttacgctgaa tagcgcaatg
    21001 caatcattta aagatgcagc acaaactgac gctgaagtga tgcaacatgt gatggatgtc
    21061 cgttccggga tgccggtgga tgtccgccgt catcaagtga gcccacaaac tctgtaaatc
    21121 ctggataaaa aaaatccccg cgacacggca tgtcgcgggg aaaaaatcag cagtagcagg
    21181 cattaatgag ggttaatgct tatttataaa ttactgctgt tccatggagg gtattcggac
    21241 cggttacaga agtaatacgg aaagattttg cgcccatctc atccgctttt tgcgccagct
    21301 gctcttccag cgatcccaga tttgtccccg cgttagcact gatagtaccg actttttgtt
    21361 ggccttctgg cgttgactga acttcgacag ccgcaaagtt ggcaaatgac atggagctta
    21421 aaatcgccgc agcgatgagg tttttacgtt tttcataata gtggccttat gcagatgaat
    21481 gacgtcgcaa gaaattactt aacgatcgat aactaaatga tagatgtgat ctggatcaca
    21541 tacaagatat tttttataac aatcattaat taatttaaaa atctttaaat ttcagtcaaa
    21601 tagaaagcaa ttatttttat gggatctgga ctggtgaacg gaggtgatta tttttataat
    21661 aacctttcat tcacagtcac caggtacaac ggcatcatgg caactgactc aacacaatgt
    21721 gtaaaaaaaa gccgtggccg cccaaaagtg ttcgacaggg atgccgcgct tgataaggcc
    21781 atgaaattgt tctggcaaca cggttatgaa gcgacttctc ttgcggacct cgtcgaagca
    21841 accggagcca aagcgcccac gctatacgcg gaatttacca acaaagaggg gttatttcgt
    21901 gccgttctcg accgctatat cgatcgtttt gccgctaagc atgaagcaca gctgttttgt
    21961 gaagagaaaa gcgtggagtc tgcgctggct gactattttg cggccatcgc caactgcttt
    22021 accagcaaag acaccccggc aggctgcttc atgatcaaca actgcaccac cctctcccca
    22081 gattcaggag atatagccaa tacgttgaaa tcacgccatg cgatgcaaga gcgcactttg
    22141 cagcagtttt tatgtcaacg acaagcgcgc ggggaaatcc cgacccactg tgacgtgaca
    22201 catctggcag aattccttaa ttgtattatt caggggatgt cgatcagcgc acgcgaaggt
    22261 gcatcgctgg aaaaactgat gcagattgcc ggaacgactt tgcgtttatg gcccgaactg
    22321 gtgaaataag tcgcaaaaaa ggcccctcag cctgagcgaa tgaggggcca aattgcagag
    22381 tacagccttg ttatactttg ttattcaaaa tcagttgccc gttactatct agcgggatct
    22441 gcgtacccgg atcgcggtcc atgcggattt tgccctgctg atcgccaatc ttataggtca
    22501 catcataacc gagcattttt tctgacttgt catacaccgt tttacaacgc tgttgcgtag
    22561 tcgtgtaagt atcgctttcc tggagagagc cctggatctg gttaccggca tatccaccac
    22621 ccagcgcccc cacaacagtg gcgacatctt taccgcgacc accaccaaac tgatgcccta
    22681 tcacgccacc agcaacagcg ccgagcaccg acccggtaat gcgattttca tcctgcaccg
    22741 gtcgacgatg ggtcactgtg acgttgcgac actcctgacg cggtgtttta accgtttcct
    22801 tgattggggt tgcagaaaca acctgagcgt attgcgggcc ccgttcaaac acgttcagac
    22861 tggccactgc cgctacgccc aacgcagctg cgacaccaat cccgataccc gccaacattg
    22921 atttattcac gggaacacct ccttctttgc agttatgcca atcatgcaag cgaaggagaa
    22981 aagcagctat cagacagaag atgaaaagcg cgggaatgac tggaaatact taaggattcg
    23041 gaggagtctg aaggaatgcc tgatgcgacg cttaacgcgt cttatcaggc ctaccgctcg
    23101 taccatttgt ccagggcgga taaggcgttt ataccacatc cgccagtgta cgtcgattaa
    23161 tgcaacttca aacgcggacg gataacgcgg ttaatactcc ccaccagcat cattaatccg
    23221 gttttaaagt aaccatgcag cgcaatctga tgcattcggt atagcgagat atatacaaag
    23281 cgcgcaattc gtccttcaat catcattgag ccgcgcgtca ggttacccat caggctacca
    23341 acggtggaga agttcgacag cgataccagc gaaccatgat ctttatactg ataatttttc
    23401 agcggcttac cgttcatctg cgccagaatg ttgttcattg cgcaagtcgc catctggtgt
    23461 gcagcctgag cacgcggcgg aacaaagccc ccttccggac gcgggcatga cgcgcagtcg
    23521 ccaatagcgt aaatgtctgg atcgcgagtg gtttgcagcg tcggttccac caccagctgg
    23581 ttgatacggt tagtttcaag accaccgata tctttcagga agtctggcgc tttgatcccg
    23641 gctgcccata ccatcagatc agcctcaata tattcgccat ctttagtgtg caggccgcct
    23701 tcatcagcac tggtgaccat ggtttgcgtc agcacgcgaa cgccaagttt cgttagctcg
    23761 ttgtgggccg cagtagagat acgtggcggt aatgcaggca aaatacgttc tcccgcttct
    23821 accagcgtta cgttcagggc ttcgttggtc aggcctttgt aaccgtagct gtgcagttgc
    23881 ttgatcgcgt tgtgcaattc agcggagagt tctacacccg tcgcgccgcc gccgacaatc
    23941 gcaatgttca ctttgccatt tgcgcccagg ttggcagagt atttcaggaa cagattcagc
    24001 atctcctggt ggaagcgacg cgcctggtgc gggttatcga ggaaaatgca gttctctttg
    24061 acgcctggcg tattgaaatc gttagaggtg ctacccagcg ccatgaccag ggtgtcatag
    24121 gcgattttac gttccggaac caacagttca cctttctcgt cgcgtagttc tgcaatagtg
    24181 attgttttcg cttcacgatc aatatcaatg acggaaccca actggaactg gaaaccatga
    24241 ttgcgcgcat gggccagata gctcaacgca tcgacgcctt catcaagcga gccagtcgcc
    24301 acttcgtgca gcagtggttt ccacaggtgg ctgtggttac gatcgaccag cgtaattttg
    24361 gcttttttct tacgtcccag cttatgcccc agctgtgttg ccatttccag cccaccagca
    24421 ccgccgccga caatcacaat ctttttcaat ggcgtagtca acgtgacccc cttaaattta
    24481 ttaaccaatt gttaattaat ggttattaac atagctctta attaacaaca ggttacagca
    24541 atgaaaatgt tcttcgctat tgagaataac atgaatggtg cattggtcat accaaaattg
    24601 atgttaatca agttttgttg cttaaaagat aggtaacttt accgggggag atcaaaaaat
    24661 agagtttatt gtttattatt tgagcaaatt cggaagggta atatagggtg aagcgtatat
    24721 cgagggcgtt tggtcgtcct gataagatgc gcagcatcgc atcaggcatt gatgctcgac
    24781 tgtcggatgc ggcgtggacg ccttatccga cttacagaat tcggagcgca tcaggtaccg
    24841 gcaaggttac ctgatgcgac gagggcattt acccgagggt tttgaacgct ttaatgcgct
    24901 gtaaatgcgg ggagatattc ttgaatttgt gcgtctgctc ttcgtcccag acaatctcgt
    24961 aataatgatg caactcttca gatgtccgct ggctgttaag tgcttcatca ttacgcgaca
    25021 aaataaccag acaacgatcg cgattcttct cgcggaagtt ggtcacacac ttagtggcaa
    25081 tatcggcata ctcttccggg cgatcaatct tgccttccat gttctcataa gggaacaaat
    25141 tagggttgaa gatcacctgg cggatatcgc aaagaaaacc aatccgttcc gcccagtatc
    25201 cgcccaggcc aacgccgcaa attagcggac gctcgtcaac gttcagttgc aacattttgt
    25261 ccacttcttt aagcagatgc tgcatatcat gtttcggatg ccgcgtactg tagcttatca
    25321 agcgtacatc cgggtcaata aactgcaatt gtaagacttt ctcgtggtta cccggactgt
    25381 tagagtcaaa accgtgtaaa tagataatca tcgcttcctc acataagcca gggttagtga
    25441 cctgctttct cttcctgcca gcgttcatgt aactgattca gacgggtgct gatcgctttc
    25501 cagcgagccg agtccatcag ttcctgtcgc gaaaatgaac ctttatgata caaacgtgta
    25561 acacgttctg ccttgatcgg tgacagatta tctaacacgc tgacggcccc tttacgatta
    25621 ttgcagacca ggatcatatc gcaacccgca tccagtgatg cctgcccgcg ttcggcataa
    25681 ctgcccataa tcgcggcacc ttccatcgat aaatcgtcag agaaaatcac gccgtcaaaa
    25741 cccagttcct gacgcaaaac ggttttcagc cagtagggag aaccgctcgc cggacgcgga
    25801 tcaacatcac tgtagatcac atgcgcaggc ataatggcgt cgagtttatt ttcgcggatt
    25861 aaggaactga agaccgacat atctttagcg cgaatctccg cttgtggacg tggatcgcac
    25921 ggtgtttctt tgtgtgagtc tgccgttact gcaccgtgtc ctgggaagtg tttcccggtc
    25981 gttttcattc cggcttcatg cataccatca ataaaccggc tggcaattgc cagggctttt
    26041 tgtggatcgg catgataaga acgctcgcca atcgccgcgc tgatatgccc gacatccagc
    26101 actggcgcaa agctgatatc aatatccata gcgatcattt cgctggccat cagccaaccc
    26161 gcctcttgcg ccagtttgcc gccctcttcc attcccaaca gcgcagcaaa ggattgtgct
    26221 gccggtaagc gggtaaaacc ttcgcgaaaa cgctgcacgc gtccaccttc ctgatctacc
    26281 gccaccacca gatgattgcg cgatgctgcg cggatctggc gcaccagttc acgtaactgg
    26341 gcaggatcat gatagttacg cgtaaagaga atcagccctc ccaccagcgg atgcgccagt
    26401 atttcacgct cttccgcgtc cagttcgtaa ccttcgacat ccaacattac tggacccaca
    26461 ctgctctcct tattgtttta ttaatagctg ccgccaggtg tcatcggcca gcctgataaa
    26521 ttgttgatcg ccggtttgtc gccagcggta ctcaaaccac cctcctttga gcatcagcag
    26581 ccagggaaac catcgcctga cctgacgcca taattgcgcc ggataaatct tcgcgcgagt
    26641 ggcatagtca ttgaccaatt gccggtgctg ttcagtattt tccacccaca ccgccgccag
    26701 ttccagcgcg atatcaccat ctccagcata ctcccagtcg atgagtttta accctgatgc
    26761 gctatgcact aaatttccgg cgtggacatc catatgcaat ggacttaagc gtaaaggtcg
    26821 tggttcccgc gctttgcgca gacgttttaa cattcgcagc caacccacag tccgccgcgc
    26881 cggatcgctt tgctgccagt acagttccag taagggcaac agcgttattc gccagccaaa
    26941 acgtggttgt tgatgtagat aatacagcaa gcctgccagt tcgttggtat ccggcaaata
    27001 cgtttttacc gcgccgggca gatagtcgac taccatccag tcacggagat ataaatgcgg
    27061 cttcggtgca atgcttgcgg gtagttgtga taaagcccga tactggcgca agaacgcgga
    27121 ctgcggcgca tcaggatcgt gcggctgacg cacaacaaaa cgctggccct gatgttcaat
    27181 gagaaaacta ccgccactaa gcccactatt aaacgtcgtg acgggatgaa actgcgggaa
    27241 aaagcgcgac agcaattcgt cgcgcgtgat gggattattg ctgcgaaacg gcacctttac
    27301 ctgaccagat aatttcgccc gtctgcacca gcatcagctg catttgtagg gtcggagcgt
    27361 taacgttgcc agaggcgctg gagtacagca cgtaatgagc gccgacattg cgggcaatgc
    27421 ctatggcttt actacgggtg cctaaactgt cctgcggcga caaacctaac tgttgcttag
    27481 ccatcgacag ttgctgggcg gaaaccaggg taaatttccc gttattagcc agcgcatttc
    27541 gcagcgtttc ggtcgcttct gcggcattca gcgaaccgtt agtacggttg ttaacgctat
    27601 caaccagcag gacgctaccc gcagtcaccc cgtcagcccc aagcatctta ctgaccatcg
    27661 gctgcattgc gccattccag tcataatggc gaatatgcgg cgcaggcggt gccgttcgat
    27721 cttcgtgctc aattgggcct ggctgctgcg ggatcgtcgg caccgagggc actgtgggga
    27781 caggctgttg tggctcggct ggttgttccg gcgctggttt cacttcttct accggtgcag
    27841 gttcacgttg ccccacacac ccggcgagaa acatcgccag cgcggtaatc aaggcgtagc
    27901 gactcatttt tgtcatcaag attcacccct ttacaaatat agataaagtc tgactttgtg
    27961 cgcccccaga aaattggcgc tgccgtacag cgttaccgcc gaatgtgcgg gaatggtaac
    28021 gctgcgtggc ctttccagag gatgcatctc cagccctctg gcgtcatacc agtaaaaacg
    28081 ataatgaacg gtaacgggtt cttgcctttc gttataaagc gttgaggatg ctgaaggttg
    28141 aatatcagac gtcgaaagga cgggcttttc tgcactgatg cccgcagcca ataaagatga
    28201 ctccatcacc agcgattgct catcattcac cggaatttcc ggatgtgaac gacagcccac
    28261 cagcagtaac aacaccagag acaccagccc aaagcatcct tttctcatcg ttacagacct
    28321 ttatgcgcca gcattggtcc cagcggacgg ccacccaaca agtgcatatg gatgtggtaa
    28381 acctcttgtc cgccatggcg gttggtattc atgatcagac gatagccatc ttcggcaata
    28441 ccttcttgct cagcaatttt tgccgctacg gtgatcatgc gtcccagcgc ctgctcatgc
    28501 tcagctgaca cgtcgttcac agtcggaatg agaatattcg gaatgatcag aatatgcgtt
    28561 ggcgcttgcg gcgaaatatc gcgaaacgcc gttaccagat catcctggta gacgatatcg
    28621 gaggggatct cacgacgaat aattttgctg aatatagttt cttctgccac gacattttcc
    28681 tttttcaaat agccatgcgt tgcgccatgc tacgccgcgc cacactgcga gtatagagta
    28741 tggtcgagtt aaacctgctc tttcaatctt aactcacgat ttcttaagca aaaaatcgtt
    28801 ttgctgccgt atatatcgcc attattccca tttctgcctg cgaatgcgct gaaaagacat
    28861 tctctgtttg gtttacacga caaatatgaa tgcgtatatt tctcatttgc atttgcattt
    28921 gcagcaaaat tattcgcaca ctaaatataa ccgctcatca ccctcctccg gcatgagcct
    28981 gacgacaaca taaaccaaga gatttcaaat gctttcaaca caatttaaca gggataatca
    29041 atatcaagtc atcaccaaac cgtcactact tgccggttgc atagcactgg cactattacc
    29101 ttctgccgct tttgctgcac cagccactga agaaacggtg attgttgagg gttcagccac
    29161 agctccagat gatggcgaaa atgattacag cgtaacgtct acctctgcgg gtaccaaaat
    29221 gcagatgact caacgtgata ttcctcagtc ggtcactatt gttagccagc agcggatgga
    29281 agatcagcag ttacaaacgc tgggcgaagt gatggaaaac acgctgggga tcagcaaaag
    29341 tcaggcggat tccgatcgtg ctctttatta ttcccgcgga ttccagatcg ataactatat
    29401 ggttgatggt atccccacct attttgaatc gcgctggaat ctgggcgacg cactttctga
    29461 tatggcactg tttgaacgcg tagaagtagt gcgtggcgcg acaggactca tgaccgggac
    29521 gggtaatcca tctgcggcaa ttaatatggt tcgaaaacac gcgacctgtc gtgaatttaa
    29581 aggcgatgtc tcggcggaat acggtagctg gaacaaagaa cggtatgtgg cggatttaca
    29641 aagcccactc accgaagacg gtaaaatccg cgcgcgaatt gtcggcggct accagaataa
    29701 cgactcatgg ctggaccgct acaatagtga aaagaccttc ttctcgggca ttgtcgatgc
    29761 tgatttaggc gatcttacga cgctttcagc cggttacgaa tatcagcgca ttgatgttaa
    29821 tagcccaacc tggggcggtt taccgcgctg gaatactgat ggcagcagca acagttacga
    29881 tcgcgcacgc agtaccgcac ctgactgggc gtacaacgat aaagagatca acaaggtctt
    29941 tatgaccctg aagcagcggt ttgctgatac ctggcaagcg acactgaatg ccacccactc
    30001 tgaagtcgaa tttgacagca aaatgatgta tgtcgatgcc tatgtaaaca aagcggatgg
    30061 tatgctggtt gggccataca gtaattatgg acctggcttt gatcatgtcg gcggcaccgg
    30121 ttggaacagt ggcaaacgta aagttgatgc gctggatttg ttcgctgacg gtagttatga
    30181 attgtttggt cgtcagcaca atctaatgtt tggtggcagt tacagcaaac aaaacaatcg
    30241 ttcagttcat gggccaacat cttcccggat gaaattggca gtttctacaa ctttaatggc
    30301 aatttcccac aagccgactg gtcatcacag agcctggcgc aggacgatac cacacatatg
    30361 aaatcgttat atgctgccac tcgtgtcacc cttgccgatc cgctgcatct gatcctcggc
    30421 gcacgttata ccaactggcg ggttgatacg ctgacttaca gcatggagaa aaaccacacc
    30481 acgccttacg ctggtctggt gtttgacatc aatgacaact ggtcgaccta cgccagctat
    30541 acctctattt tccagccgca aaatgatcgt gacagttcag gcaaatatct ggctccaatc
    30601 accggtaaca actacgagct gggtctgaaa tcggactgga tgaatagccg tctgaccacc
    30661 acgttagcca tcttccgtat tgagcaggat aatgtcgctc agtccaccgg tacacctatc
    30721 cccggcagca acggcgaaac cgcctataaa gcggtggatg ggacagtcag taaaggggtg
    30781 gaatttgaac tcaacggcgc aattaccgac aactggcagc tgacatttgg cgcaacgcgc
    30841 tatcttgcag aggataacga aggaaacgcc gttaatccta atctgccacg caccacggtt
    30901 aaaatgttca ccagctatcg gttgcctgtc atgccagagt tgacagtcgg cggtggtgtt
    30961 aactggcaaa atcgcgtgta taccgacacc gtgacaccgt atggcacctt ccgcgccgag
    31021 caaggtagct acgcgctggt ggatctcttc acccgctacc aggtgacgaa aaacttctcg
    31081 ttacagggga acgtcaataa cctgttcgac aaaacctacg ataccaacat ggaaggttct
    31141 atcgtctacg gcgcaccgcg taatttcagc attaccggca cgtatcaatt ctgaataaca
    31201 cctgtaaaaa aggcagccat ctggctgcct tagtctcccc aacgtcttac ggattagtgg
    31261 ttacggatgt actcatccat ctcggttttc aggttatcgg atttagtacc gaaaatcgcc
    31321 tgaacaccag aaccagcaac cactacgccc gctgcgccca gtttcttcag gccagcctga
    31381 tccactttag acacatcagc aacgctgacg cgcagacggg taatacatgc gtcgaggtta
    31441 gtaatgtttt ctttaccacc aaatgcagca accagagccg gtgccatttc gctggtacct
    31501 gttgcttttg catcttcagt cgcgtcttca cgacccggcg ttttcagatc cagtgcttta
    31561 atcagcacgc ggaagatggt gtagtaaaca atcgcataac cgataccgac gatcggtaac
    31621 agccacagtt tgctgctgtt accagacaga acgatgaagt cgatcagacc gtgcgagaac
    31681 gacgtaccgt cacgcatccc cagaagaata cagattggga atgccaggcc tgccagaatc
    31741 gcgtggatga tgtacaggat cggcgcaacg aacatgaaga agaactcgat cggctcggtg
    31801 ataccggtca ggaacgaggt cagcgccgcg gagatcataa taccgcccac tttcgcacgg
    31861 ttttctggtt tagcagagtg ccagatagca attgcggcag ctggcagacc gtacattttg
    31921 aacaggaagc caccagacag tttacccgca gtcgggtcac ccgccatata acgcggaatg
    31981 tcgccgtgga aaacctgacc tgctgcgttg gtgtattcac caatctgcat ctggaaaggt
    32041 acgttccaga tgtggtgcag accaaacggt accaggcaac gttcgatgaa accgtaaatg
    32101 ccaaacgcaa ctaccgggtt ctggtaagca gcccactgag agaaggtctg gattgcagaa
    32161 ccaatcggcg gccaaatgaa ggacagcaca acgccagtaa agatggcagc caggccagaa
    32221 atgatcggca caaagcgttt accggcaaag aagccaagat actcaggcag cttaatacgg
    32281 tagaaacggt taaacatgta cgctgcaatc gcaccggaga taatccctcc gagtacgcca
    32341 gtatccgcca agtgtttaga ggcgatttct tcagcaggta aatgcagtac cagtggcgca
    32401 accacggcca tggttttaac catgatgcca taggcaacaa ctgcggccag cgcggatacg
    32461 ccatcgttat tggtaaagcc gagggcgaca ccgatcgcaa aaatcagtgg catgtttgca
    32521 aagacggaac cgcctgcttc tgccataaca tgcgatacaa cggcgggcag ccagctgaaa
    32581 ttcgcggaac cgacgcccag cagaatacct gcgataggta gtacggatac cggcagcatc
    32641 agcgatttac cgaccttttg caggttagca aatgcattct taaacataat tgagagtgct
    32701 cctgagtatg ggtgcttttt ttacgttctc acgcgtggca aggggggaga gcctcgccgt
    32761 gtacagggca tctaagcgcc ctttatttat tacacagagt aaaataattc agtgccaatt
    32821 tgtttgacgg ctatcacgtt tcaacttcag atcattggag aagttgcaca ttttgacaaa
    32881 acatatgtca caatatttca aacgcggtta ttcaccgccc aattttcggc ggtgaacttt
    32941 actcgtttta gccattaatt acgagcttta aaaaaactca ttcaacggat ggattgaagg
    33001 cgggaagcgt cgatgtgaaa cagacgggcg aagttatcgg tggttacctg cgccagttct
    33061 tcaacggcaa cacctttcaa cacagccatg tattctgcaa cgtcacgaac catcgcaggt
    33121 tgattctctt ttcctcgatg cggtaccggc gcaaggtaag gtgagtcagt ttccaccagt
    33181 aatcgatcca gggggacata acgcgcagca tcgcgcagtt gctccgcatt acggaaggtc
    33241 acaatgccgg aaaaggagat gtaaaatccg agatccagta atttacccgc cgtttctctg
    33301 tcctctgtaa aacagtgtag tacgccaccg caatccgtca ctttttcttc gcgcagaatt
    33361 gccagcgtat cggcacgggc gtcacgggta tggacgataa ccggcttgtt cagttcacga
    33421 ccaatctgga tatgatggat gaaggactct tgctgacgta ctttagtttc cggtgtgtaa
    33481 taataatcca gcccggtttc acccagcgct acaacaccct cttctgccgc cagacggcgt
    33541 aaatcttcta catcgtaggg atcattctgg ttgagtggat gcacgccaca agaaaatacg
    33601 acgttgtcac gttcgcctac cagatcccgc atatgtaaat aacccggtaa tgttgtggcg
    33661 actgccagac aaaatttcac atcgcgtgcg gcggctttcg ccagaacgtc atccacgtcc
    33721 ttatgcaaag attcataatc cagaccatcg agatggcagt gtgagtcgac taaaaacatg
    33781 atgtctctct taaagatgag gaaccggtag cacaacgccc ggttgcaggt aatgctcaat
    33841 acgcagtaaa agatcggtga tgagaagctc gcggttgatg cctgtgacag acattaactg
    33901 ttcacgaatg tggcaaacat cccccagtat agcctgcagg cgcgagggag aaagatggtt
    33961 tgccagttcg gcgaccagtc ccggcacatc aacattggtc acctgcgcag cgccatgatg
    34021 gcgttttagc gcatccatca gcaacgttgc cagccagtgt aaacgcgccg gagcttgttc
    34081 atgattaagg gccgccagca gcgaatacca gtcgcccgat ggcacgctat atgccaacgc
    34141 ctgacacaat gtttcacgag cctgccagtt atctccctga aacaacgcca gtgccgcgcc
    34201 aggcgaaccg gcgcttaagc gcaatgcggc aagtaatgca tcctgtgaca ttgtcacttc
    34261 gcgtgaaagc caagtcacgg cgtactgttc tggaggcggc gcaaggtaat gtaaccgaca
    34321 acgactacgt aatgttgcca gtaaacgttc aggctcgcgg gtagccagga aaaaccaggt
    34381 ttctgctggt ggctcttcaa gcgttttcag caatgcgtta gccgcggcgt cggtcagtaa
    34441 ggcagcatcg gttacccaga caactttcgc accacctagg cgtgcgtgct cattcagctt
    34501 ttcggtgacc tcacgtaccg catcaatgcc cagcgtattt ttcccttttt cgggagccag
    34561 ggtgtagtaa tcgggatgcg tgccagcctg catcaactga catccacgac agtgaccgca
    34621 acttttgtgg ccctgcggtt gttggcagag taaataacgg ctcagggcgt agattaaagc
    34681 atcatcgccc atgcccggta acgcctgaat gagtagcgca tggtgacctc ttccggcctg
    34741 atagctggct accagttttt cgaaatcagg tcgtaaccat ggataccatc tcatgcgtcc
    34801 aactccttca cccagtgggt cacggtagtg cggattgcat ccatcacggc ctccagcggc
    34861 tgggtggcat caatggtatg aatactttta tcttgtgcag ccagttccag atagcgggcg
    34921 cgggtgcgat taaagaaatc gaaagactct tgctcaatac gatccagctc gccgcgcgca
    34981 cgcgcgcgtt ttaagccaac ttccggggta acatcgagat agagcgttaa gtctgggcga
    35041 aaatccccga gaacagcatc acgcagtgtt gccagcatat gttggtcaat accacgtcct
    35101 ccgccctgat acgcctgagt ggaaagatcg tggcgatcgc caatcaccca ggtgccgtta
    35161 gccagcgcag gtttgatgac cgtttctacc agttgaacgc gcgcggcata aaacatcaga
    35221 acttcggctt tatcggtaat gacttcatcg cctaccgatt tgatatccag caccaggctt
    35281 cttaactttt cggcaagttg cgtaccgcca ggttcccgag tgaaaaccat gtcgcggata
    35341 cccagttgct cgagcgtctc aaccaccaca ttacgcgcgg tagttttgcc tgcgccttcc
    35401 agcccctcaa tgacgatata cttactgcgc atttttttcc ttaagcactt tcagataatc
    35461 ctgcacagac ttgttatgac tggcaagatt ggtattaaac gtgtgaccac ctttaccatc
    35521 ggccacaaaa tagagatacg gcgtttttgc cggatgcgca gcagccttca gcgaatccgc
    35581 ccccggcgta gctatcgcac ccggcggcaa accggtaatg gtataggtgt tatacgctgt
    35641 cggcgtttcc aggtctgcac gagaaagttt gccattataa cgctctccca tcccgtaaat
    35701 cacggttggg tcggtctgca ggcgcatacc aatgcgtaaa cggttgataa ataccgaggc
    35761 aacctgatcg cgttcactgg caacggcggt ttctttttcg ataattgatg ccatcgtcac
    35821 cagctgattt ttatctttat aaggcagacc gtccgcacgc ccttcccagg cgctatcgac
    35881 cgctttcacc attttcttgt gaactcgctt gagtaacgcg acatcggtgg tattggcggt
    35941 atacatcaag gtgtctggcc agaaccaacc ttcaatccac tccgggtttt ccagttcaag
    36001 tgcctgcgct acggtggcgt acttatcgtc actcagcgtg tgcttgatat acggagcctc
    36061 acgcaattgc ttgaggtagt cgctcagacg catcccttct accagtcgca gcgggaactg
    36121 tgcttcttta ccgctttcca acaatttcag catctcgcgc acggtcatct gcggtgtaaa
    36181 gcggtaagtc ccggctttaa agtgagaaag atccggttcg atacgcagca gccattgaaa
    36241 aacccgtggg cgattgatga tcttatcagc ataaagctgt tcaccgagcg ccagacgtcc
    36301 ggtccctggc ttcagggtaa atatcgtctc ttctttgata agcaatttgc tgtcggcaag
    36361 atggcgaacc ttccagacgc ccacaccagc ggcgataccc agtaccacca gcaataacaa
    36421 gataatcaat aacacttttt tcatgactaa ttcgggcgct cacaaagtgg ggctaaatat
    36481 tcatataacg ttgctgacga aaaggtgaca tcgccacagg cacgtacggg catcactggc
    36541 attaacgcat tacaaataac catctcatct gcctgcaacg cctcctccag cgaggcttgc
    36601 acttcgacaa gctgataaga ggattgtgcc agtaaacgga tacagaattg tcgcataatg
    36661 ccgttaacac ctgcctgatc cagtcgcggc gtataaacta cattgccctt ccgccagaac
    36721 aaattagccg cacagcattc cgtaacccac ccttcgctgt caaggaccag cgcctcatca
    36781 gcgtttgtct gctcaagatg agagcgaatc aatacttgct caagccgatt aagatgttta
    36841 atacctgcaa gatgaggatt gcgccccagc cgcaccgggc ttagcgccaa cgtcatccct
    36901 tcgttacgca aacggtcgta atgtgcagga taagccgtaa cggagagaat ccgcgttgct
    36961 ggtccgctgt tcaatgtgct gtaccctcgc ccgccactac cgcgactgat cacgactttc
    37021 agcacaccat tttgctgttc tgctgccagc gttttcatct cttgttcaag ctgaggccag
    37081 aagtcacagg aaatcatcaa ccgctgacaa gcatcctgta gtcgcaggat atgcgccgat
    37141 aacaaactga ctttaccgtc gataactctg gcggtggtaa aacaaccatc accaaactgc
    37201 gttgcccgat cgcttactgc cagcgattcc tgcttataac cgttaattaa gaacataccg
    37261 gctccttatc agagaattgt tagtgtggca gcatgttcac tacggaacaa gtcggaataa
    37321 aagctaagaa aaaaggcccg caagcggacc ttttatatgg gtggaaaatg acaacttaga
    37381 tctttttaaa gatcaaagaa ccattagtgc caccgaagcc gaaggagtta cacagcgtgt
    37441 attccattcc gctgacctga cgtgcttcat gcggtacgaa atccagatcg caaccttcat
    37501 ccgggttatc caggttgatg gttggcggaa cagcctgatc gcgcagcgcc aggatggagt
    37561 agatagattc tactgcaccc gccgcaccta acaggtgacc ggtcatagat ttcgtggagc
    37621 ttaccaacac acggcttgca gcttcaccga agatggtttt caccgcctgc gcttcagctt
    37681 tatcgccagc cggcgtagaa gtaccgtgcg cgttaacgta gccaatctga ctcgcttcaa
    37741 tgcctgcatc acgcagagca tttgccattg ccagagctgc gcctgcgcca ttttctggcg
    37801 gtgacgtcat atgataagca tcgctgctca taccaaagcc gacgagttca gcgtaaattt
    37861 tcgcaccgcg ttttttcgcg tgttcgtact cttcaagtac cagcataccg gcaccatcgc
    37921 ccagtacgaa gccatcacgc tctttatccc acgggcggct cgccgcttgc gggttatcat
    37981 tgcgggtaga taatgcacgt gccgcgccaa aaccaccaac gcccagcggc gtactggctt
    38041 tctctgcgcc acctgcaacc atcacgtcag catcgccata cgcgataata cgcgcagcat
    38101 ggccaatgtt gtgcacgccg gaagtacagg cggtcgcgat agagatgctt gggccacgca
    38161 ggccatacat gatagtcaga tgacctgcca ccatgttcac aatcgttgac ggaacgaaga
    38221 atgggctgat cttacgtgga ccaccgttca tcagagatgt gtggttttct tcgatcagtc
    38281 cgaggccgcc aatcccggag ccaattgcgg caccaatgcg ggttgcgttc tcttccgtta
    38341 tttcaaggcc agaatcctgc atggcctgaa cgccagcgac aattccatat tgaatgaagg
    38401 catccatctt gcgctgttct ttgcgcgaga taatgtcctc acagttaaaa tcctttacta
    38461 agccagcaaa tttcgttgca taggcgctag tatcgaaatg gtcgattagg ctgatgccac
    38521 tctgaccggc aagcagagct ttccaggtag actctacggt attgccgaca ggagacaaca
    38581 tgcccagtcc ggtcacaact acacgacgct tagacacgtt tgtcctccaa ggagggaaaa
    38641 atgattctag tgggacaaaa agataaaact caggcggtcg aacgaccgcc tggagatgtt
    38701 cacttacgcc tggtggccgt tgatgtaatc aatggcagcc tgaacggtgg tgattttctc
    38761 agcttcttcg tccggaatct cagtatcaaa ctcttcttcc agagccatta ccagctcaac
    38821 ggtgtcaaga gaatccgcgc ccaggtcttc aacgaaagaa gcattgttgg taacttcttc
    38881 ctgcttaacg cccagctgtt cgccgataat tttcttaacg cgttcttcga tagtgctcat
    38941 actcttaaat ttcctatcaa aactcgcttt cgcgatggtt ttcgtagtgt ataaaatgtt
    39001 gaaaaatttg caactaaatc ccggcaggtc ttaccacgat tttacgttat tttgaggcct
    39061 taccccccaa taacgcaaat cattttgcac taattattac tcataaccac gcagactgcg
    39121 cgcaacatga gcattttgtg caaatcgcgg tcagaccatg tacatcccgc cgttcacatg
    39181 caaagtttca cccgtgatgt aagctgcttc gtcggatgcc aggaatgcaa ccgcgttggc
    39241 gatttcctgt gcgccgccga ggcgacccgc aggaacctgc gccaggatac ccgcacgctg
    39301 gtcatcgctc agcgcacgtg tcatgtccgt ttcaataaag cccggagcaa caacgtttac
    39361 agtaataccg cgtgacgcaa cttcgcgcgc cagtgattta ctgaagccga tcaagcccgc
    39421 tttcgccgca gcgtagttgg cctgaccgcc atttcccatg gtaccaacca cagaaccgat
    39481 agtgataata cgaccatgac gctttttcat catagcgcgc attaccgctt ttgacagacg
    39541 gaaaacagat gaaaggttag tttcgataat atcgttccac tcttcatctt tcattcgcat
    39601 taacaggtta tcacgagtga taccggcatt attgaccagg atatccactt caccaaattc
    39661 tgcgcgaatt ttttccagaa cagattcgat agatgccggg tcggtcacat tcaacatcag
    39721 acctttgccg ttggcaccta aataatcact gatcgcctga gcgccatttt cactggtcgc
    39781 agtgccaata actttcgcgc cacgggctgc gagcgtttca gcaattgcgc ggccaattcc
    39841 gcggcttgca ccggttacca gtgcgatttt tccttcaaaa ttcatgattt tcctctttta
    39901 aagctcgagc gccgctgcca tcgctgaagg ttcgttcagc gccgaggcgg tcagggtgtc
    39961 gacaatgcgt ttcgtcaggc cagtaagcac cttgcccggg ccgacttcat agagatgttc
    40021 tacgccttgc gctgccatgt actcaacaga cttcgtccac tgaaccgggt tatacaactg
    40081 acgtaccagt gcgtcacgga tggcatcacc attggtttcg catttcacat caacgttatt
    40141 cacaacagaa actgttggtg cgttaaaggt gattttcgct aattctactg ccagtttgtc
    40201 ggctgctggt ttcatcagcg cacagtgaga cggtacgctc actggtaacg gcagcgcgcg
    40261 ttttgcgcca gcagctttac aggcagcgcc agcacgctca accgcttctt tatgaccggc
    40321 aataaccacc tgtcccggag agttaaagtt taccggagaa acgacctgac cttctgcagc
    40381 ttcttcacac gctttcgcaa taggcgcatc atccagaccg atgattgccg ccatagcgcc
    40441 cgtgccttcc ggtacggctt cttgcatgaa cttaccgcgc atctcaacca gacgcaccgc
    40501 atcagcgaaa tcaatcacgc cagcgcaaac cagcgcggag tattccccca ggctgtgacc
    40561 ggccatcatt gccggtgctt taccgccctg ctgctgccat acgcgataca gcgcaacaga
    40621 tgcagtcaac agcgctggct gggtttgcca ggttttattc agttcttcag ctggcccctg
    40681 ctgggtcagc gcccacaggt cgtagcccag cgccgcagaa gcttcagcaa acgtttcttc
    40741 gacaattgga tagctcgccg ccatatcagc cagcattcca acggtttgag aaccctgtcc
    40801 agggaacaca aatgcaaatt gcgtcatgtt ttaatcctta tcctagaaac gaaccagcgc
    40861 agagccccaa gtgaatccac cgccaaaggc ttcaagcaga accaactgcc ccggcttaat
    40921 gcgcccgtcg cgtacagctt catccagcgc gcacgggaca gaggccgcag aggtattacc
    40981 gtggcgatcc agcgtcacca cgacattgtc catcgacata ccgagttttt tcgccgttgc
    41041 actgataata cgcaggttag cctgatgcgg aaccagccag tccagttgag aacggtcaag
    41101 attattcgcc gccagcgtct catcaacgat gtgcgccagt tccgttaccg caaccttgaa
    41161 gacttcgttg cccgccatcg tcagatgaat tgaattctct ggattcacgc ggtcggcgtt
    41221 tggcagcgtc agcaattcac cataactacc gtcggcatgc agatgggtgg aaatgattcc
    41281 cggctcttca gaggcagcca gcaccgcagc gcccgcgcca tcgccaaaaa taataatagt
    41341 cccacgatct gttggatcgc aggtgcgcgc cagtacatcg gaaccgacga ccagagcata
    41401 cttcaccgcc ccagatttca cgtattgatc ggctacgctt aatgcatagg tgaaacctgc
    41461 acaggctgct gcaacgtcaa atgccgggca acctttaatg tccaacatgc tttgaatctg
    41521 acaagctgcg ctcgggaaag cgtgcgtagc agaagtcgtt gccacaacga tcaggccaat
    41581 ctggtctttc tcaatgcccg ccatctcaat tgcgcgtgtc gccgcttcaa agcccatggt
    41641 tgaaacggtt tcgtttggcg cggcaatgtg gcgttcgcgg ataccggtac gagtgacaat
    41701 ccactcgtca gaggtgtcca ccattttttc caaatcggcg tttgtccgca cttgttcggg
    41761 cagatagctg ccagtaccaa taatcttcgt atacatgtac gctcagtcac ttttcggtta
    41821 tataccgtca cttgcaaact gcgagttcgc tggcagcgtc ctgctaccgc agagttccgc
    41881 ttttgccacc gtccagcagc tcaaaaccag ctgggtatac agattccagg cgagcggcaa
    41941 ttcgctgagg aacttgtcgc tgcaccgcct gcactgcctg ttcaatcgcg accgcaaaag
    42001 ctcgctgatt ggctgcacca tgacttttta tcaccgtgcc gcgcaatcct aacagacagg
    42061 cgccgttata ctggtcgggg ttgaggtgac tgaatcgcct cgtcaggctc ttttgtagcc
    42121 aacgctttaa taacagtagc caccacgacc gttttttccc ttcaccctga gatttcagca
    42181 gagaaaggaa catcctgaca acaccttcca tcgtctttaa cgtgacattt cctgtaaagc
    42241 cgtcacaaac cagcacatct gtcttgccag ttaacaactc attggcttca agatagccga
    42301 tataattgat agaagggatt gttttaagca ccgctgaggc atcccgaata ctgtcaagac
    42361 cctttacttc ttcttcacca atattgagca acgccacgcg aggattggga atttccacca
    42421 cctcttcagc cagaactgag cccataatgg caaattgcac caacattgtg ctgtcacaat
    42481 cgacgttggc ccctaaatcg aggaccaccg ttttgccctt ttgctgatgt ggtaataccg
    42541 tcaccagcgc cggacgctca atcccctcca ggggcttgag taataatttt gccagcccca
    42601 tcagtgcccc ggtattaccg gcactgacac aggcttgcgc tcgaccttct ttcaccagct
    42661 ccagggccat acgcattgaa ctcccacgac tggcgcggat agcttgcgaa ggccgggcat
    42721 cactggcgat aactgactgc gcaggaataa tctgcagacg cgaacgttgt tcaaagtcag
    42781 ctttagcaag taatggcgtg atggcgtcgg gattgccgac taaaagaaga gtgagttgcg
    42841 aattagaatt cagtgcctgc aatgctgcag gcactgtcac ggaagggcca aaatcccctc
    42901 ccatgacatc taacgccagg gttagacgtg tcaaggtatc gtcgccgccc ggtttggtct
    42961 ttccccagtt gcctggggaa aatcctcact aagcttcatc acgcagacgc gtgattactt
    43021 agcgatgacc ttgcggccgc ggtagtaacc gtcggcagtg atgtggtgac gcaggtgttt
    43081 ttcaccagaa gttttgtcta cagacaggct ggtgactgcg gtcagcgcgt catgggaacg
    43141 acgcatgcca cgtttggaac gggttggttt attctgttgt acggccatgg accttactcc
    43201 tcaattacgg tttatccccg atccaacggg gagcaccaat tacttacgct ttaagctggc
    43261 taatacggca aatgggtttg gcttttgcac ttcttcaggc agttcaccaa agaccatgtc
    43321 cgcttcggac acttcacagt gttcagaatc atgcaccgga actaccggca aggcgaggat
    43381 gatttcatct tcaaccattg caagcagatc gatttcaccg aattcgttaa cctcaatcgg
    43441 ttcatacgct tccggcagtg cttcagcctg ttcgtctgaa cgcacaggac taaaacaata
    43501 cgttgtgtag acctgatgag taaacggctt cccgcaacgc tgacactcga gcgttaccgt
    43561 caccttcgca tcgccgttta acactgcgag acgttggtta tcgatagcga acgacatgga
    43621 gcattccaca tcactgtcca cactgactac ggattcggcg acgcgctcaa cctgatcagg
    43681 ggtatagata ccctggtaat caaggcgttt ttgagccgta cgaaccggat cgagagtcag
    43741 gggtaatttt accttttgca tagggcgcgc atattaactt tgtaacgtca tagagtcaaa
    43801 gaaaaaggca gcctgtggtt gccttttgcc aataattcgc acacattgcg ggttactgct
    43861 ttattttcgt tcagtgctga taacgaacat caacctgcaa aacgaagcgc atagtttaaa
    43921 atggctatca tcaatacgct atatctgatg gaaaaaaata tgcctaaact tattttagcc
    43981 tccacctcgc catggcgtcg cgctcttctg gaaaaactgc aaatctcttt tgaatgtgca
    44041 gcacctgagg tcgacgaaac cccacgcagc gacgaatcac cgcgacagtt ggtgcttcga
    44101 ctggcacaag aaaaagcgca atctctggcg tcacgttatc cggatcattt aattattggt
    44161 tcagaccagg tatgtgttct tgatggcgaa atcaccggta aaccgttaac ggaagaaaat
    44221 gcccgtctgc aattacgcaa agccagcggc aatatcgtca ccttctatac tggactggcg
    44281 ttgtttaatt cggcgaatgg gcatctacaa acagaagtgg agccttttga cgtccatttc
    44341 cgtcacctaa gcgaggcgga gattgataat tacgtgcgta aggagcatcc cctgcactgc
    44401 gcgggtagct ttaagagtga aggatttggc attacgctgt ttgagcgttt agagggacgt
    44461 gaccctaaca cgctggttgg tttaccgcta atcgcgctgt gtcagatgtt acgtcgggaa
    44521 gggaaaaacc cgctgatggg atagttgttt gcgccggatg cggttacgtc gcatccggca
    44581 atcggtgcag acggcctgat gcggcgtaaa cgccttatca ggccaaccgc ccattatcag
    44641 gcttatatgc ttgtattagc gcgcgttacg cagcttttgc aaacaacgct tcaaaccttc
    44701 atccatcggc gcttcgatac gcatcacctc accggtcccc ggatgggtaa acttcaacgc
    44761 tgcggcgtgc aggaacagac gatttaatcc cgtgcctgct tcagtgagct gtctgtcaaa
    44821 ttcacggtca ccgtagcgat cgtcaaaggc aatcggatga cccgcatact gtgtatgcac
    44881 acggatctga tgagtacgtc cggttaccgg gctacaacgc accagggtgg caaatgcata
    44941 gcgttcttcc actttaaagc gtgtttccga cggtttgcct tcctgactga cacgcacgat
    45001 acgttcgccg ctttgcagaa tatttttcaa taacggcgct tgaacgctct tcacatgcga
    45061 ctgccactga ccgcgcacca gcgccaggta atctttttgc attccttttt cacgtaattg
    45121 ctcatgcaga gaacgcaacg ccgagcgttt tttcgctacc agcaacacac ctgaagtgtc
    45181 ccggtcgaga cgatgaacca gttcaaggaa ccgcgcttcc gggcgcaacg cccgcaaacc
    45241 ttcgataacg ccgaagctta aaccactgcc gccatgtacc gccgtaccgg aaggtttatt
    45301 cagcaccagg atatgatcat cttcatataa gatgacgtcc gccagcgccg ccaccttttg
    45361 cagatgtggc gaaaccgcct cttcttcccg ctcagcaacg cgaaccggtg ggatacgcac
    45421 ctcatcaccc gcttcgagtt tatattcagg cttaatacgt tttttgttca cccgcacttc
    45481 acctttacgc aaaatacggt aaatcatact ttttggtacg cctttcaatt gggtacgcaa
    45541 aaagttatcg atacgttgcc ccgcttcgtc agcggtgata gcaacaattt ttacggatgg
    45601 agtctctgtt ttcatggtgg gcgattctaa ttagccaaca ggattcgcgc cactcatttt
    45661 tctatgctta tatttagttt tgcaccttat tacttcacta cgtgatcact ttattgatgg
    45721 ttattaaacc aatcaccagc aagaagtgaa aaaactgtga gtaagcgggt gataaatggt
    45781 aaaagtcatc ttgctataac aaggcttgca gtggaataat gaggccgttt ccgtgtccat
    45841 ccttgttaaa acaagaaatt ttacggaata acccattttg cccgaccgat catccacgca
    45901 gcaatggcgt aagacgtatt gatctttcag gcagttagcg ggctgcgggt tgcagtcctt
    45961 accggtagat ggaaatattt ctggagagta atacccagtc tgtttctttg ataattgcgc
    46021 tgtttttccg catgaaaaac gggcaaccga cactctgcgc ctctttgagc tgacgataac
    46081 cgtgaggttg gcgacgcgac tagacacgag gccatcggtt cacacccgga aaggcgttac
    46141 tttgcccgca gcttagtcgt caatgtaaga ataatgagta agttacgatg aaaagaatgt
    46201 taatcaacgc aactcagcag gaagagttgc gcgttgccct tgtagatggg cagcgtctgt
    46261 atgacctgga tatcgaaagt ccagggcacg agcagaaaaa ggcaaacatc tacaaaggta
    46321 aaatcacccg cattgaaccg agtctggaag ctgcttttgt tgattacggc gctgaacgtc
    46381 acggtttcct cccactaaaa gaaattgccc gcgaatattt ccctgctaac tacagtgctc
    46441 atggtcgtcc caacattaaa gatgtgttgc gtgaaggtca ggaagtcatt gttcagatcg
    46501 ataaagaaga gcgcggcaac aaaggcgcgg cattaaccac ctttatcagt ctggcgggta
    46561 gctatctggt tctgatgcca aataacccgc gcgcaggtgg catttctcgc cgtatcgaag
    46621 gcgatgaccg taccgaatta aaagaagcac tggcaagcct tgaactgccg gaaggcatgg
    46681 ggcttatcgt gcgcaccgct ggcgtaggca aatctgctga ggcgctgcaa tgggatttaa
    46741 gcttccgcct gaaacactgg gaagccatca aaaaagccgc tgaaagccgc ccggctccgt
    46801 tcctgattca tcaggagagc aacgtaatcg ttcgcgcctt ccgcgattat ttacgtcagg
    46861 atatcggcga aatccttatc gataacccga aagtcctcga actggcacgt cagcatatcg
    46921 ctgcattagg tcgcccggat ttcagcagca aaatcaaact gtacaccggc gagatcccgc
    46981 tgttcagcca ctaccagatc gagtcgcaga tcgagtccgc cttccagcgt gaagtccgtc
    47041 tgccatccgg tggttccatt gttatcgaca gcaccgaagc gttaacagcc atcgacatca
    47101 actccgcacg cgcgacccgc ggcggcgata tcgaagaaac tgcgtttaac accaacctcg
    47161 aagccgccga tgagattgct cgtcagctgc gcctgcgtga cctcggcggc ctgattgtta
    47221 tcgacttcat cgacatgacg ccagtacgcc accagcgtgc ggtagaaaac cgtctgcgtg
    47281 aagcggtgcg tcaggaccgt gcgcgtattc aaatcagcca tatttctcgc tttggcctgc
    47341 tggaaatgtc ccgccagcgc ctgagcccat cactgggtga atccagccat cacgtctgcc
    47401 cgcgctgctc cggtaccggt accgtgcgtg acaacgaatc gctgtcgctc tctattctgc
    47461 gtttgatcga agaagaagcg ctgaaagaga acacccagga agttcacgcc attgttcctg
    47521 tgccaatcgc ttcttatctg ctgaatgaaa aacgttctgc ggtgaatgcg attgaaacgc
    47581 gtcaggacgg tgttcgctgc gtgattgtgc caaacgatca gatggaaacc ccgcactacc
    47641 acgtgttgcg cgtgcgcaaa ggggaagaaa cgccgacctt aagctacatg ctgccgaagc
    47701 tgcatgaaga agcgatggcg ctgccgtctg aagaagagtt cgctgaacgt aagcgtcctg
    47761 aacaacctgc actggcaacc tttgccatgc cggatgtacc gccagcgcca accccagctg
    47821 aacctgccgc gactgtcgta gccccagcac cgaaagctgc aacggcaaca ccagcagctc
    47881 ctgcacaacc tgggctgtta agccgcttct tcggcgcact gaaagcgctg ttcagcggtg
    47941 gtgaagaagc caaaccgacc gagcaaccaa caccgaaagc agaagcgaaa ccggaacgtc
    48001 aacaggatcg tcgcaaacct cgtcagagca accgccgtga ccgtaatgag cgccgcgaca
    48061 cccgtagtga acgtactgaa ggcagcgata atcgcgaaga aaaccgtcgt aatcgtcgcc
    48121 aggcacagca gcaaactgcc gagacgcgtg agagccgtca gcaggctgag gtaacggaaa
    48181 aagcacgtac caccgacgag cagcaagcgc cgcgtcgtga acgtagccgc cgccgtaacg
    48241 atgataaacg tcaggcgcaa caagaagcga aggcgctgaa tgttgaagag caatctgttc
    48301 aggaaaccga acaggaagaa cgtgtacgtc cggttcagcc gcgtcgtaaa cagcgtcagc
    48361 tcaatcagaa agtgcgttac gagcaaagcg tagccgaaga agcggtagtc gcaccggtgg
    48421 ttgaagaaac tgccgctgcc gaaccaattg ttcaggaagc tccagctcca cgcacagaac
    48481 tggtgaaagt cccgctgcca gtcgtagcgc aaactgcacc agaacagcaa gaagagaaca
    48541 atgctgataa ccgtgacaac ggtggcatgc cgcgtcgttc tcgccgctcg cctcgtcacc
    48601 tgcgcgtgag tggtcagcgt cgtcgtcgct atcgtgacga gcgttatcca acccagtcgc
    48661 caatgccgtt gaccgtagcg tgcacgtctc cggaattggc ctctggcaaa gtctggatcc
    48721 gctatccaat tgtacgtccg caagatgtac aagttgaaga gcagcgcgaa caggaagaag
    48781 tacaagtgca gccgatggtg actgaggtcc ctgtcgccgc cgctgtcgaa cctgttgtta
    48841 gcgcgccagt tgttgaagaa atggctgaag tcgtagaagc ccccgtaccg gttgccgaac
    48901 cgcaaccgga agtggttgaa acgacgcatc ctgaagtgat tgccgccgcg gtaactgaac
    48961 agccgcaggt gattaccgag tctgatgttg ccgtagccca ggaagtcgca gaacacgcag
    49021 aaccggtggt tgaaccgcag gaagagacgg cagatattga agaagttgcc gaaactgctg
    49081 aggttgtggt tgctgaacct gaagttgttg ctcaacctgc cgcgccagtc gtcgctgaag
    49141 tcgcagcaga agttgaaacg gtggccgcgg ttgaaccaga gatcaccgtt gagcataacc
    49201 acgctaccgc gccaatgacg cgcgctccgg caccggaata tgttccggag gcaccgcgtc
    49261 acagtgactg gcagcgccct acttttgcct tcgaaggtaa aggtgccgca ggtggtcata
    49321 cggcaacgca tcatgcctct gccgctcctg cgcgtccgca acctgttgag taataattag
    49381 ctcaaagtaa tcaagccctg gtaactgcca gggctttttt atttcatgtt tgaatcaaca
    49441 tattaaaatt catcaaatgg catccttgct aaccaacaat gcaaaatagg caaaaaaatg
    49501 cccgccccgg cgatcccaga gcgggcagac aaaacatacc cagtttcatg atatgtttta
    49561 aagcgagctt atttgttgag ctggaagagc gacaatccct gcatatcggt aaatgcttta
    49621 tacgatgcct gcaatgccgt ttgctgcatg atgtaagatg aaatagttgc attccagtcc
    49681 acatcaacca gatcgctcat ctgctgcgtt tgccctaaag cgcgatcgct acctaaggaa
    49741 tccagcgact ccagtttgtt cagctgcgtg cctaattccg cgcgaacggt aaggacatta
    49801 ttcagcgagt ttttcagtcc acggttggtt ttatccaatg ccgcagcggc gatttcttta
    49861 tccgcttcgc tatccgcgac cggcgttttc agggctgcga tggcgctatc cagcatggca
    49921 aaaagattgg tttcagaagc gctaccgtct ggttccgcta ccgcgttact ggtaatactg
    49981 tcgaaaattt tgtcgcccgt atgtcctatc accatcgaac gcgaagcatc gacctgttgc
    50041 ttaatacttt ctgttccgcc aacataatca ccgttagcct cactaaacgg cgcagtctct
    50101 gttttataac cggcaaaaat gtagcgccca ttaccgtcag tggtgttcgc cagattcagc
    50161 aactggtcac gaagcccctg aatatccgta gccagcgagg cccggtcatc gtcactcaag
    50221 gtgccattgc tggcgtagac aattttttcc tgagcattct ggatagcagt ggtgacctgg
    50281 ctaagtacac tctcttccag tgacactttt tgagtggcga aagtacgtgc cagcgtgtac
    50341 tggctgtttt gcgcctgtgc ctgggagaga acaacagctt gtgatgcagc aatgggatcg
    50401 tctgaagggt taacgactcg cttacccgtc gacatctgct cgccgtactt catccattct
    50461 gcctgagaat tggtgatgcc gcgcatgttt tgctggtaca tcatctgggt actgaaacgc
    50521 attttatctc cccttagcga atgttaatca gcgcatcaaa aatcgcgttt gccgtctgca
    50581 gaacctgcgc attcgccagg taatactgct gaaaacgttg cagatttccg tactcctcat
    50641 cgagattgac accggaaatc gactgctgct gattggaaag ctgcgtcacc acattacctt
    50701 gcgtggtgct actggttttc aacgtcgcgg ttttattacc gatatcactc actaacgagg
    50761 cataagcgtc gttaaaggat ttcgcaccgc ccaccgtttt actgttgctt tgcagatcca
    50821 gcagggcctg accgttgcgg ttgtcgctat cacccgcatc ttcttcgctc gccatcgcga
    50881 ttttcgcttc gtcggtgatt aatacatcca tgttgacgat ggcgtcactt actggtttca
    50941 gcgtgaagct gtcgttaacg gcaggcgttc ctgtaaacgt caactccaga ccatcaaatg
    51001 ccactttacc gttggcatct ggtgtcgccg taaaagtggt attgctggca aggcgggtga
    51061 cctgccactg attattatcg aacgagattt tgtaatctgt cgccagtacc acggaggcat
    51121 cagttaccgt ggcaccgatc gcaacgtcac ctttgttttt cgtgttttgc agaaccgcgg
    51181 gcttaccgat agtaaagaaa tcttcaccag catcgccgtt agcatcaaat ccggctttgt
    51241 gttggctgtt gaaagcctcg gcaaatgcca gcgccagttg tccaagcgta ttacgcgtct
    51301 ggtccagatc ctgagaacgg aatgtcagaa tgccgcccag cgacccggta ttcagtaatt
    51361 tctccgggat ctcaatattg cctgccgtcc catcaacgta agcgacagtc gtacgagaag
    51421 ggtcggcgct ggaaggaact gccgccagtt gccgcgccgt acttccctga accagtgagt
    51481 aaccattggc atcgtgatgt tataagtacc gccatcctga acgctgactt ctacgccaac
    51541 aatctggttt aattcgctca ccagttgatc gcgttgatcc agcagattgt taggtgacgc
    51601 ccctgccccc acgcctgtca ggcgcgagat ttgatcgttc aggctggcaa tttgtttagc
    51661 gtaattgttg atctgatcaa cgctggcacc tatcgcgata ttgacctgtt tgtcctggtc
    51721 gcgcagatat tgatcggtgg ttttaaactg attcaccaac ccttctgatt tcccaatcag
    51781 cgcctggcgc gccgccgggt cttccgcgtt actcaccagc gtttgcaggc tggtgaagaa
    51841 atcctgcatc tgtgttgcca gcgaagaggt actggtggag agcatattgt cgattttcga
    51901 catctgctca tagcgggcgg tcaggccgct actttgcgtc tgcgcagcac gtaactggtt
    51961 ggtaataaac gtatcatact cacgctgcac accagaaacg tagacgccat tgccaaccca
    52021 gccgccagcg cccaacgtgc tattggcctg cgccataata gtggtttggc gggtatatcc
    52081 ggcaacgtta tagctggaga tattattact tgccgtattt aacgccgcct gggccgcgtt
    52141 cagtccgctc atggcgttat tgatcaagct ggacatggag gttccttata agccttcaat
    52201 tacagagtat tatcggcagc gacccgccgg acttgagtta ttcagaacag attatcaatg
    52261 ttcatgctgt aggttttgct caccttgtcg cttatcgatt tcatctgctg gatcatgttg
    52321 gtgagtttac gggcatagtg aggatcggta gcatagcccg cgtcctgtag gacctgcgcc
    52381 ccctgttccg cactcgcggc ggtcgtcacg gcggcgtagc gcgggttacg tgttaacagc
    52441 ccaacgtaat ccgacaatgc ttccagatac gagctgtaga cccgaaactt cgcttttact
    52501 ttcttcgctt cgccgttttc atattcagtc gtggtgattt cagtgacttg ccctttccag
    52561 ttgccagagg ctttgacacc aaacaggtta tagcttggct cgccgttttc gcggcggatt
    52621 tgccgttgcc cccaaccaga ttcaagcgca gcctgagcga ggatcaaatg atgtggcaca
    52681 ccgctttgct ggcttgccag ttgggcgggc agcgagagtt gcgcgaggaa tgctctactg
    52741 tcacccggca gcgaatcatc gtagttacgt ggtacggcct tttgcaccag ctgcgaaagc
    52801 gtctggtttt gataacgcac cacggtttcg agcgggaatt tcatcggtgc tgctggcgtg
    52861 gactcctctg gcaatggttg ttctggcgtc atctgtttaa ccatcatctc tgcaagcccc
    52921 agacctttgc ccgccgtcat ctgttgggca atttgctggt catacatact ggtatacaga
    52981 cgagtgtgct cgctgctgaa caggccatct tttggtaaag cgtcgcgcat gcttttcaac
    53041 atcatctgca cgaacatccc ttccacctga cgggccaccg gacggatatt tgccgccgga
    53101 tcttcgctcg ctttcgcctt tagttcgttg agtgattgcg catcccaggc cgcacttgcc
    53161 agtagtttgc tgtcgctgat catcagatga tttccagttt tgcccgcaga catcccgcac
    53221 tttgcattga ttgcaaaata gacatcagat ccatcggcgt agcgcccagc gcattgagcg
    53281 cacgcaccac gttattgagg ctggcgctgg aacgtacgct ttgcagcgaa ccgccgctct
    53341 ggcgtaaatc gatctgcgtt tgtggagtaa caacagtctg tccaccacca aacggtgtat
    53401 ctggctggct gacattggcc tgacgattaa ctgttactga gagatttccc tgcgctaccg
    53461 cgcagctgtc gagggtcact tcgcgattca tcaccaccga accggtgcgc gagttaatca
    53521 ctactttagc gtcctgcggg gtgacattaa cctgcatatt ctggatatcg gcaaggaaac
    53581 ggacctggga actgttgcca cttggtacgc gcacctgaat agtccgcgca tctaacgcgg
    53641 tggcgctgcc atatccacgc acgcggttga tggtgtcagc gatttgctgc gccatgctga
    53701 aatcttcgtc gttaagttgc aaattaaggg tattcccgac gccaaactgg ctgggcaatt
    53761 cacgttcaat aaccgcacca ttggtgatcc gtccaccgtt cagttggtta acctgaacac
    53821 tgctaccgcc tgcggaggct cctgcgccgc caaccagaat attgccctgc gccagcgcat
    53881 acacctgact gtcaacgccc ttaagcggtg tcatcaacaa cgtacctcca cgcaagcttt
    53941 tggcattacc catggaagaa accaccacat cgatggtttg cccctgacgt ccaaacggag
    54001 gaagtgacgc tgtcaccatt accgcagcga cgttttttag ctgcatattg gtgcccgtcg
    54061 gaacggtaat tcccagctgt gagagcatgt tattaagcgt ttgtgtggta aacggcgtct
    54121 gggttgtctg gtcaccagtg ccatccagcc ccactaccaa gccatagcca atcagtgagt
    54181 tttgccttac cccctgaaca ctggtgagat cgcgaatacg ctcagcctga gccgccgtcg
    54241 taaccagtag aagaattaat gcagagagaa atttaatcac cacagcctca cttacattgg
    54301 cgacaggtta aggaagaaac gctgcaacca gcccatattt tgcgcttcgt taatgtagcc
    54361 attgcctacg tattcaatac gcgcatccgc cacctgagta gacggtacgg tattgctgcc
    54421 gctgatagtg cgtggattaa ccacgcccga gaagcgaata aattcggtac cctgattaat
    54481 ggcaatctgt ttttcaccca ccacatgcag gttgccgttg accagtacct ggtcaaccgt
    54541 caccgtcaac gtgccgctaa aggtattgct ggcattggcc ccgccctttc cgttgaacgt
    54601 gttaccaccg gaggcttcga catcggcacg agcgttacca aacagcccct gcaaatagcg
    54661 cggcacagta tcaaagccaa aattagtttt accgtcacgg ctggcattcg cagaggagct
    54721 tttgctggcg ctgacgttct cctgcaacac gatggtcagc gtatcgccaa tattgcgtgg
    54781 tcgacgatct tcaaacagcg gttgatagcc atagttaatc ggctgagcag actggaaaat
    54841 agaaccgttg gcgacgggcg tcggaccggg aaccggttgt gcactggtcg ccccctgcac
    54901 cagcggcgtg gagggtatcc aggcgcagcc ggttagtgaa agcaccaaca agctggaaat
    54961 ggcataagta tgcgcagcgt ttttttgcat ggctatcatc ttcaaaaatc agtaaaccgg
    55021 tgaacctgcc accggttatg ccttagagtt gcgtcagttt ttgcagcatc tgatcggtgg
    55081 tggacaccgc tttactgttg atttcgtaag cgcgttgcac ctgaatcata ttgaccagtt
    55141 cttccgccac gttgacgtta gacgtttcaa catacccttg atacagcagt cccgcgccgt
    55201 tcaggcccgg cgtgctttcg ttcggtgcac cggaggattg cgtttcggtg tagaggtttt
    55261 cgccaatgct ctccagtccg gtatcattca taaaggtggt gagattgagc tgcccaacct
    55321 gaaccggagc tgcctggcct tgttgggtta cgctgaccac gccatcacga ccgatggtga
    55381 tacttaacgc attcgccgga atggtgatcg ctggctgcac ctgaaaacca ccagccgtca
    55441 ccagctgccc gttctgatcc acctggaaag agccgtcacg gatataggct gatgagccat
    55501 ctggcaacat tacctggaaa aagccctgcc ctttaatcgc gacatctttg ctgttattgg
    55561 tctgcgacag attgccctgg ctgtgtaagc gttcagtggc gaccgggcgt acccccgtgc
    55621 cgatttgtaa tccggagggt aaggtggttt gttcggaaga ctgtgccccc ggctggcgaa
    55681 tggtttgata aagcagatct tcaaacaccg cgcgctgacg cttaaaaccg ttagtactga
    55741 cgtttgccag gttgttggca atgacgtcca tattggtttg ctgggcgtca aggcccgttt
    55801 tggcgatcca taatgaactg atcatataat gtcctgtagt ggattgctga caagcagagc
    55861 agaagcacgc gacagtgtga atttcccctc accctaaccc tctccccaaa ggggcgaggg
    55921 aatagtccgc gcgcttttat tgcaatgtca tcaatcgttc aattaactca tcgacagcag
    55981 ttggttggcg cggcctgcgt tatcatcgac gctgctgatc accttcatct gcatttcaaa
    56041 acgccgcgcg ctggcaatca tgtcgctcat tgccgcaacg gcattgacgt tactgccttc
    56101 cagaaccccc gacatcacac gcaaggttgg atctgcctgc agtaccggcc ctcgcgtggc
    56161 ctgggtttct gcgcttaaac gaaaaatgcc gtcgtcaccg cgctgcactt cgctgcccgt
    56221 ggctttcacc agtttcagac gccctactgg cgcaactgta tttgccggat cgcccggatt
    56281 gagcgccgag attgtgccat cggcagcaat cgtgatttcc gccccttccg gcacagcaat
    56341 tggccccgcc tcgcctatca ccggatgccc ctgaattgtc agttgcccgg tgggatcaac
    56401 ctgaatgctg ccattacgcg tatacccttc gctgccgtca gcggtctgca cggccagcca
    56461 tcctgctgca acgcaacgtc cagcgggcgc gaggtgtaat ccatcttgcc gggcgtcatg
    56521 tctgcacccg gtgttgacgc cgttaccagc gtgcgcgtgg gcagtgaaag cccttccacc
    56581 ggcactgcgc gcaaggcgtt taactgcgcg cgaaaaccgg gcgtagaggc attggccaga
    56641 ttactggcgg ttaccgcctg ttgattcagc gtctggctgg ctgctcccat cgcagtataa
    56701 attgcgtgat ccattgagct atcccgtcag cgattagcgt aagttaaccc gcgtgttgag
    56761 gatctggtcc tgggttttga tggtctgggc gttagactta tagttacgct gggcaacgat
    56821 catattgacc agttctttac tgagatcgac gttggacgct tccagcgcac cgttggtcag
    56881 ggtgccaaag tttcccgtcc cggctgtccc caacagcgcc acgccagaag attgcgtcgc
    56941 agaccagacg ttgtcgcctt cggatgccag accttcgttg ttggcaaagt tcgccagtac
    57001 aatctgcccc agcagttggg tttgttcgtt ggaattgttg ccgacaaccg taccgtcatc
    57061 attgatttga taactcacca gatcgcccgg tttgtagcca ttctgggtgg ttgccacaat
    57121 gttgttagcg ccggtatttt gctgcatgga gttgaggaag ctcagactaa acgtggcggg
    57181 ttctgcaccg ttaattgcgc cggttgcgat attattcgcc atcgcaccat ccactaatgt
    57241 gccattagca ttaaattcca gtgttgtcgc tgtcttcgca atgctgtttg gatcactgct
    57301 atcctgggtg tagacctgcc agttattatc cccggtcttc acaaaataga cgctcatgtc
    57361 atgagcatta ccctgactgt cgaaaacagt caccgaacct tttttgttat agctatccgc
    57421 attgctggcg ctgaatggcg taacagtagg aagcggatca ctggaattca ggttgatctg
    57481 catcgacgcc gtggtggtag ttttcgctgc catcagggta ttcgggatcg aaatattggt
    57541 cggattcgcc ccttgctgaa tagtcggcgg cgtaccggtt accgggtaac ccgtcagctg
    57601 taaaccttgc gtattcaaca ggttacggtt ttcatccagc ttaaattgtc cgttacggct
    57661 gtagaacacc gaaccgttgc tgtctaccag acggaaaaaa ccgttctggc tgatagcaac
    57721 gtccagacct cgcccggtgt tggtggtcgt gccatcggta aagtcctgag tgataccggc
    57781 aacttttacc cccagtccca ctttcgaacc ggcaaacata tcggcaaaag aggccgtgcc
    57841 tgatttaaag ccgtaggtgg cggagttggc gatattgttg ccaataacat cgaggttggt
    57901 ggcggcagcg tttaatccgc taaccgcttg agaaaaggcc atgactgact cctgaaatgt
    57961 gaaggcttaa attatctgcc gtacttcgtc gagggtggtg gtgccgtaag tgccgagatc
    58021 cagcgtatta ccgctgttgc cgcggatcac accctgcacc agagcaaact gcagcggctg
    58081 ggcaaccagt tgtgtaccac cgttactggc gctaatcgct acattgtaag aaccgttcgg
    58141 cgcagtgctg ccatcagtca acgaaccgtc ccaggtgaaa ctgtgaactc cggcggtcag
    58201 ttcaccaata tcaatggtgc gcacaaccgc gccattttta tcggtgatgg tggccgtaac
    58261 tttgtctgcc gcctgttgca gctcaacacc aaacggcgtg gtcgtggtca cagccccttc
    58321 ttcactgccg gttcccgcaa gaacagttgt gccggggatc atcacgccgt gaccgatcag
    58381 gttactggcc tgtaacgact ggctgttatc aatctgtccg gaaatagatc cgagcgtggt
    58441 attgagtttt tcaatcccgc tgaccgtgct gatttgtgcc aattgcgacg tcagctcgtt
    58501 gttttccatt ggattggtcg ggtcctggtt tttcagctgc gccaccagca aagtcagaaa
    58561 actgctttgt aaatctgcgg cgttgctgcc cgtgagcgaa ctactgctgg tggtactgac
    58621 gccggtattt gtcggatcgg tggtggttac cgcaatggac atagctttct cctttattga
    58681 ccgagcgtaa gggttttcag catcatgctt ttcaccgtgt tgagcacttc aacattggcc
    58741 tgatagctgc gtgacgccga catggtgtta accatctccc cgacgacatc aacgttcggc
    58801 atttttacgt agccctttgc atctgccagc ggattacctg gttcataaac cagtttgtcc
    58861 ggggcctgac tttcttaaca tcggcaacct ttacgccgcc tgtcgcagca cctggtgcag
    58921 cgttaacctg gaataccacc tgttttgccc gatatggctg tccatcagga ccggtcacgc
    58981 tatcagcatt tgccagatta ctggccacca cgttcaggcg ctgggactgg gcagttaacg
    59041 ccgacccggc gatatcaaaa atattcagca gtgccatccg ttaatttccg ctctgtaaaa
    59101 cgttcatcat gcctttgatt tgcccgctca acgcgctaag gctcatctgg tattgcaggc
    59161 tgttatcggc aaactgggtg cgttcgcgat ccatatcgac ggtattaccg tcaagcgaag
    59221 gctggtccgg aatacggtat tgcagttctg cggtaggagg cgtcagcgcc tgcgccggga
    59281 tgtgttgcgt tgaggtcatc gtcagtgcaa ccacactggt tgcatcccgt ccacgttgca
    59341 tgactttttt aagttcactg gcaaaatcga tatcgcgcgc ctgataacca ggggtatcgg
    59401 cattggcgat gtttgctgcc agcacttcct gacgctgggc gcgcagattg agcgcctctt
    59461 gttgaaaacg taaggcggcg tcgagcttat cgagcatatc tcctccgcag gtatcaaaat
    59521 tctgccatca gcttaaatgc ctttacgcgc gcgttatcgg cggaataaac gcaaaatggg
    59581 tcgctattta tgccgttgat ggtcattgcg gacaggtaca attcacgttg tagaaatggc
    59641 tgggggcgaa aatgctgaca ataaaacgta gcgtggcgat catcgcgata ctgttcagtc
    59701 cgttaagtac ggcgagcaat ctcacatcgc aattgcacaa cttttttagc gcccaactcg
    59761 cgggggtaag tgatgaggtt cgtgtttcta ttcgtacagc gcccaatcta ctaccgccat
    59821 gcgagcagcc attgctttcg atgagcaata attcccgcct gtggggcaat gtgaatgtgt
    59881 tggcacgctg cggtaacgac aaacgatatt tacaggttaa tgtgcaggcc acaggaaatt
    59941 atgtggttgc cgcgatgcct attgtgcggg gaggaaagct ggaagctggc aatgtcaaac
    60001 tgaaacgtgg acgcctggat accctgccac cgcgtacggt gctggatatc aatcaacttg
    60061 ttgatgccgt tagcctgcgc gatctatcac ccgatcaacc tatccagtta acccagtttc
    60121 gccaggcatg gcgggtaaaa gcgggacaac gcgtcaatgt gatcgccagc ggtgatgggt
    60181 ttagcgccaa cgcagaaggt caggcgctga acaatgcagc cgtcgcacag aatgcgcggg
    60241 tgcgcatggt atcgggacag gtagtcagcg gcgttgttga tgcagatggg aatattctta
    60301 taaacctgta agctgttaaa gattacccgt cccttgccga taaataagca acacatgata
    60361 aaagcgccct caatgaggaa taaaccatga gtattgatcg cacttcgcct ctgaagcctg
    60421 taagcaccgt tcaaccgcgc gaaaccaccg acgcgccggt aacgaacagc cgggcggcaa
    60481 aaacaaccgc ctccaccagc accagtgtga cgttaagcga cgcgcaagca aaactgatgc
    60541 aacccggcag cagtgatatc aatcttgaac gtgtcgaagc gttaaaactg gcgattcgta
    60601 acggtgaact aaaaatggac accggcaaaa ttgccgatgc gctgatcaac gaagcgcagc
    60661 aagacttgca gagtaactga tcgtatgaca cgtcttgcag agatcctcga ccagatgtcc
    60721 gctgtgctta acgatctcaa aacggtaatg gatcaagagc agcaacatct ctctatgggg
    60781 cagatcaacg gcagccagtt gcaatggatt acagaacaaa aaagctcact gctggcgacg
    60841 ctggattacc tcgaacagtt acgcaagaaa gaacccaata cagcaaatag cgttgatatt
    60901 agtcaacgct ggcaggaaat tactgtgaaa acgcagcaac tacgccaaat gaatcaacat
    60961 aacggctggt tactggaagg acagattgag cgcaatcaac aggcgctgga aatgttgaaa
    61021 ccgcatcagg aaccgacgct atatggggcg aacggtcaga cctcaacacc ccatcgcggc
    61081 agtaaaaaga tttcgatctg aatattatct ccggcctgca ctgcaggccg gaatgcattg
    61141 ttacaccgtc cggcgggcaa attctttaac tttgaagccc agtaccgcca gtgcagcgaa
    61201 gtacgcggca atccccgcca gcacgaccgc cattaaacgc agtaagcgcc agggcatggt
    61261 acccaatgac cactccggca tgatatgtaa catacctaaa agcacgccag acatcaccag
    61321 taccgccacc accagacgca acagaaacgc catccagccg ggttgcggag taaagatttt
    61381 ctgcttacgc aactgccagt aaagcagcga agcattcaga cacgccgcca gaccaataga
    61441 aagtgacagc ccggcatgtt tcaacggacc aataaacgcc aggttcatca attgcgttaa
    61501 aatcagcgta acgatggcaa ttttcactgg cgttttaatg tcctggcggg aataaaagcc
    61561 aggagccaac actttcacta caatcaggcc aatcaaaccc accgagtagg caattaacgc
    61621 ccgctgggtc atcagcgcat caaacgcggt aaatttaccg tactggaaca gcgaaacggt
    61681 taacggacct gaaagaatgc ccaacgcaac cgcactcggc agtgccaaca ggaaacaaag
    61741 acgcaacccc cagtccatca aacggttgta ttcatcatga ttgccactgg caaaactttt
    61801 cgacagcgac ggcagcaaaa tggtgccaag cgccacgccc agcacaccgg agggaaactc
    61861 cattaagcgg tcggcgtaat acatccaaga caccgaaccg gaagcaagaa acgaggcaaa
    61921 aatggtgttg atgattaagg agatctggct cacagagacg ccaaggatcg ccggtcccat
    61981 ctgtttcacc acgcgcattg ctccagcatc gtggaagtta atgcgcggca ggaccagcat
    62041 gccgatcttc ttcaggtgcg gtagctgata caccagctgc aggatgccgc cgaccgtaac
    62101 agcccacgcc agcgccagca ccggtgggtt aaagtacggt gcggcaaaca gcgcgaaacc
    62161 aatcatgctg atgttaagca gtgttggcgc aaacgccgga atcgagaagc ggttccacgt
    62221 attcagaatc gctcccacca gcgacgccag ggagatcagc aagatatagg gaaaggtaat
    62281 ctttagtagc tggctggtca gggcaaattt gtcagctgtg tcagcgaagc ctggcgcggt
    62341 caccatgatc acccacggtg cggcgagcat gccagcgacc gtcacaaccg ccagcgcaag
    62401 tgtcagcagg ccagaaacat aagagacaaa gacccgcgtg gcgtcttcac cctgcttact
    62461 tttatattcc gccagaatcg gtacaaatgc ctgggaaaat gccccttcgg caaagatacg
    62521 gcgtaacaag ttaggaagtt taaaagcgac gaaaaaggcg tcggttgcca tccctgcgcc
    62581 aaagattctg gcgacaattg cgtctcgtgc gaagccaagc acacgcgaaa acatggtcat
    62641 cgagctgacg gcggccagcg attttaataa attcatcggt gttctaatcc ggacccacgg
    62701 gcaaaacgcc tgcaaggcag gcgctgaaag aggcgaatag tctacctaga ttatggtgaa
    62761 ttgctaccgc cagatgttac agggtttatt cactcatcgc atcgcgccag atcttgtcaa
    62821 cgatacgttg cgccagcact gcctattcgc cggcggtttg cggaactgtc tgattttgca
    62881 cgcattcaat gaagtgccgc gcacagccga caaatccacg ttgctcaaga gtgctctgcc
    62941 aaccaggaat cggtttattc actacaccct gcccgcgctc ctcacgccat tcgcgcatat
    63001 ccgtaatgtc gatgagcgca ccgtcagtca cggcctgcac ggtttcacgc tgacttccgg
    63061 cacggcgatg catacaagtg gtgatctgca aaggaccggc cgaaaaatgg tgctcggcaa
    63121 acagcatttc gccagcgtcg ttagtcagta gcgtaccgcc atccagagag gctttgccgc
    63181 ccgacaacca cagcgcggta tccaccacat gcagataatc atccagcaac gtgaaataaa
    63241 gatcgtgtgg cccgacgcta ttgctacgat gtttatccat tcttagcgag gctgcggtgg
    63301 cgagttgcgt ttttaactca ccgtagagtg gtgcgaaacg acggttaaaa ccgaccatca
    63361 acgtcagttt tttccgcgcc gccagttcca ccagcctttc agcatcgcgc agattttctg
    63421 ccagcggttt atcgacacag acatgtaccc ccgcattgag taacgtactg accacgtcaa
    63481 agtggctggc ggtgctggag tgcacaaaaa ccgcatcaca actggcggca aggctggata
    63541 acgaatcggc ataaggaatg cgccagctgt cacaaattgg cagggctttg gcgcgcgtag
    63601 gcgaccaggc tccttgtaac gtccagtcag acgctgccgc cagcaccggt aaccacgctt
    63661 tttgcgcaat gccgcctaat cccactacgc cgatacgtaa ttttttcact ttaatctccc
    63721 agatgggcca gcaacgaatc aagacgttgt ttcagttctg ccacttcgat ttccagggct
    63781 tcgacgcggg tctataaatc accgtcaacc atgttcgaca tagccgttac cgccggctga
    63841 tcttcaacct caccactgaa aagatgcatg tagcggcttt cgcgtttacc cggttcgcgg
    63901 gccagacgca ccacaaaagg accatcttcg cgattcgcca gttgttccag cgtcgactcc
    63961 acttccgcca tatcgctgaa ttcatacatt cgcgcggcgc ggctgcgcag ttcacccggc
    64021 gtctgggcac cacgcaataa caacgtggtg attaacgcca cttctgcggc gctcagtttc
    64081 agatcgccaa attctgaatt acaaaaacgt tgctcatatt tggtgacccg attaccaaaa
    64141 ccgctcactg tgcgtagata atgacgtttg accagattat ccagctgttc ctgcacttcg
    64201 gattcactca gattcatgac cggtttacgg ttcgttttct gattacaggc cgtgactaca
    64261 ccattgactg agagcgggta ttgctccggc gtggtcacct gcttttccag caagcaacca
    64321 attacgcgag cttccaaggc agtaagttga tatttcatga gattctcctt agcggccggg
    64381 cgtccagtct ggggtagtta atgccgtcag tacgtgatcg cgccattgtc catcaatcaa
    64441 cagatagtct ttcgcatagc cttctttttc aaaacccagt cgcgccagta aatcaccgct
    64501 gcgtttattg tgcggcatat aattagccat aatgcgatga atatgttggg tgcgctgcat
    64561 ataacgaatg gctgcggtca gggcttcaaa catgagtcct ttgccctgcc atttttgccc
    64621 aatcgaataa ccgagatagc aggcatgaaa agagccgcga acaacattgg aaaaattggc
    64681 aacgccaata atctcttttt cgtccgggtc gaataagcca aagtagaaag ctgaaccttg
    64741 tttatgaaat tcgttaatca tccctagcct ggcctgccag cctgatggat aacagtggct
    64801 ttcgtcgcgc actggctccc agggcttgag gaaatggcga ttctctgcgt aataatccgc
    64861 cagacgccag gcatcacgat catgcaccag acgcacgacc agtcggtctg tggttaagcg
    64921 cacttttggc acgttactgc gatagccaaa cattctatac ctactccttc ccgtaacgtc
    64981 tttaatacgc tgtaagtatt actatacctg cgcaaccaga ccctgtgaaa agtccaaaat
    65041 gccattttct gcattattca catatttgat aaaaactctc tatcaaagcg acgttttgat
    65101 aaaaaaatat tgtccccgaa gtgatgggaa aagtcttagc ggcaatgcag aatagaaagt
    65161 gctcatcttt tatttcccgg gaggggaaat gtcccgcgta tcgcaggcga ggaacctggg
    65221 taaatatttc ctgctcatcg ataatatgct ggtcgtgctg gggttctttg ttgtcttccc
    65281 gctgatctct atccgcttcg ttgatcaaat gggctgggcc gccgtcatgg tcggtattgc
    65341 tctcggtcta cgccagttta ttcagcaagg tctgggtatt ttcggcggcg caattgccga
    65401 ccgctttggt gccaaaccga tgattgttac cggtatgctg atgcgcgccg ccggattcgc
    65461 cacaatgggt atcgcccacg aaccgtggct attgtggttt tcatgcctgc tctcgggact
    65521 cggcggcacg ttgtttgatc cgccgcgttc ggcgctggtg gtgaaactaa tccgtccaca
    65581 acaacgtggt cgttttttct cgctgttgat gatgcaggac agtgccagtg cggtcattgg
    65641 cgcattgttg gggagctggc tgttgcaata cgactttcgc ctggtctgcg ccacaggggc
    65701 cgttttgttt gtgctatgtg cggcgttcaa tgcgtggttg ttaccggcat ggaaactctc
    65761 caccgtacgc acgcccgttc gcgaaggcat gacccgcgtg atgcgtgaca agcgttttgt
    65821 cacctatgtc ctgacgctgg cgggttacta catgctggct gtacaagtga tgctgatgct
    65881 gccaattatg gtcaacgacg tggctggcgc gccctctgcc gttaaatgga tgtatgccat
    65941 tgaagcgtgt ctgtcgttaa cgttgctcta ccctatcgcc cgctggagtg aaaagcattt
    66001 tcgtctggaa caccggttga tggctgggct gttgataatg tcattaagca tgatgccggt
    66061 gggcatggtc agcggcctac aacaactttt caccctgatt tgtctgtttt atatcgggtc
    66121 gatcattgcc gagcctgcgc gtgaaacctt aagtgcttcg ctggcggacg caagagctcg
    66181 cggcagctat atggggttta gccgtctggg tctggcgatt ggcggcgcta ttggttatat
    66241 cggtggtggc tggctgtttg acctgggcaa atcggcgcac cagccagagc ttccgtggat
    66301 gatgctggga attattggca tcttcacttt ccttgcgctg ggttggcagt ttagtcagaa
    66361 acgcgccgcg cgtcgtttgc tggaacgcga cgcctgattt gatactcccc cctgactcct
    66421 ccatactgaa agtgaactga cagccaaatg gaggagtcac gtgaagctat acatttacga
    66481 tcactgccct tactgcctca aagcccgcat gattttcggc ctgaaaaata tccccgtcga
    66541 attacatgtt ctgctcaacg acgacgcaga aacacccacc cggatggtcg gtcaaaaaca
    66601 ggttcccatt ctgcaaaaag atgacagccg ctatatgcca gaaagcatgg atatcgttca
    66661 ctatgtcgat aaactcgacg gcaaaccgtt actgaccggc aaacgttccc ctgccattga
    66721 agagtggctg cgcaaggtca atggctacgc caacaaactg ctgttgccgc gttttgccaa
    66781 atcggcattt gatgagtttt ctactcccgc cgcgcgcaaa tatttcgtcg acaagaaaga
    66841 ggccagcgcg ggtaattttg ccgacctgct ggcccactct gacggtctga ttaagaatat
    66901 cagcgatgat ttgcgtgcgc tggacaaact gatcgtcaaa ccgaacgccg tgaatggcga
    66961 actttcggaa gatgatattc agctatttcc gctactgcgt aatctgacgc tggtagccgg
    67021 aatcaactgg ccaagccgcg ttgctaatta ccgcgataat atggcgaaac agacacaaat
    67081 caatttgtta tcatcaatgg cgatttaatc ctgacccgcc gcggcagcct gtattcgcgg
    67141 gttgccgccg ttttcagttt tttctgctgg cgagtttatc gctggtgaca catgctgcgt
    67201 tgtgtcagga tattgacgca gatttcatgt cgaggactac catgaacaag tttttatttg
    67261 ccgctgcatt gatcgtcagt ggcctgctcg ttggctgtaa tcaactcacc caatacacca
    67321 tcaccgaaca agaaattaac cagtcgcttg cgaaacataa taatttctca aaagatatcg
    67381 gtttacccgg cgtggctgac gcccatattg ttctgacaaa cttgaccagc caaattggtc
    67441 gcgaagagcc gaataaggtt accctaaccg gagacgccaa tctggacatg aactccctgt
    67501 tcggtagtca gaaagcgacc atgaaactga agctgaaagc gctgcccgtg tttgataaag
    67561 aaaaaggtgc gatcttcctg aaagagatgg aagtggtcga tgcgacggta caaccggaaa
    67621 aaatgcaaac ggtgatgcaa acgttgcttc cctatttgaa ccaggcatta cgcaattact
    67681 ttaaccagca acctgcttac gtcctgcgcg aagatggcag tcagggcgaa gcaatggcga
    67741 aaaaactggc gaaaggcatt gaagtgaagc caggcgaaat tgtcattccg tttacggatt
    67801 aatgacgagg gcgcattcgc gccctttatt tttcgtgcaa aggaaaacgt ttccgcttat
    67861 cctttgtgtc cggcaaaaac atcccttcag ccggagcata gagattaatg actgcaccat
    67921 cccaggtatt aaagatccgc cgcccagacg actggcacct tcacctccgc gatggcgaca
    67981 tgttaaaaac tgtcgtgccg tataccagcg aaatttatgg acgggctatc gtaatgccca
    68041 atctggctcc gcccgtgacc acagttgagg ctgccgtggc gtatcgccaa cgtattcttg
    68101 acgccgtacc tgccgggcac gatttcaccc cattgatgac ctgttattta acagattcgc
    68161 tggatcctaa tgagctggag cgcggattta atgaagccgt gttcaccgct gcaaaacttt
    68221 acccggcaaa cgcaaccact aactccagcc acggcgtgac gtcaattgac gcaatcatgc
    68281 cggtacttga gcgcatggaa aaaatcggta tgccgctact ggtgcatggt gaagtgacac
    68341 atgcagatat cgacattttt gatcgtgaag cgcgctttat agaaagcgtg atggaacctc
    68401 tgcgccagcg cctgactgcg ctgaaagtcg tttttgagca catcaccacc aaagatgctg
    68461 ccgactatgt ccgtgacgga aatgaacggc tggctgccac catcactccg cagcatctga
    68521 tgtttaaccg caaccatatg ctggttggtg gcgtgcgtcc gcacctgtat tgtttaccca
    68581 tcctcaaacg caatattcac caacaggcat tgcgtgaact ggtcgccagc ggttttaatc
    68641 gagtattcct cggtacggat tctgcgccac atgcacgtca tcgcaaagag agcagttgcg
    68701 gctgcgcggg ctgcttcaac gccccaaccg cgctgggcag ttacgctacc gtctttgaag
    68761 agatgaatgc tctgcagcac tttgaagcat tctgttctgt aaacggcccg cagttctatg
    68821 gcttgccggt caacgacaca ttcatcgaac tggtacgtga agagcaacag gttgctgaaa
    68881 gcatcgcact gactgatgac accctggtgc cattcctcgc cggggaaacg gtacgctggt
    68941 ccgttaaaca ataaaaatcg tagcttcctg ttgtcattag gttattttac ctgtataaat
    69001 aaccagtatg ttcaacaggg ggctattatg cgaattgaag tcaccatagc gaaaacttct
    69061 ccattgccag ctggggctat tgacgccctg gctggcgaac tttcccgccg tattcagtat
    69121 gcgtttcctg ataatgaagg ccacgtatcg gtacgttatg ccgcagcgaa taatttatcg
    69181 gttattggcg caacaaaaga agataaacag cgcattagcg aaattctcca ggaaacgtgg
    69241 gaaagcgccg atgactggtt tgtcagcgaa taatatgcag tgatttttgt ttttgccgga
    69301 tcgccccggc ttttttattt tacttacgaa ttttgagtac aaaatttcta cacttcctca
    69361 aaataattgc acaatatgct aaaaaaatat gaatgacatg ctgttttatt gttcatataa
    69421 atcttaaaag cagaaattta tctgttcccc cctttaaaaa ttcggtgaat acccttactt
    69481 attggtatac tgaagttgct tcagataaaa acgcattgaa cctcgaataa cgttgtctag
    69541 taacacgaat tagggggcca tgatggaaaa aaataatgaa gtcattcaga ctcatccgct
    69601 cgtagggtgg gacatcagca ccgttgatag ctatgatgcg ctgatgttgc gtttgcacta
    69661 ccagacccca aataagtccg agcaggaagg gactgaagtt ggtcagacgc tctggttaac
    69721 cactgatgtt gccagacaat ttatttcgat attagaagca ggaatcgcca aaattgaatc
    69781 cggtgatttc ccggtaaacg agtatcggcg tcattaacat gacatgacca tccacccaaa
    69841 aggcacctca ccggtgcctt taccatttct gggttgtata tctgcattca gttaattact
    69901 ctgcttaaac catgagagca gagagaaaaa aatgaaatac gatctcatca ttattggcag
    69961 cggttccgta ggcgctgccg ccgggtatta tgcaacccgc gccggtttaa acgtgctaat
    70021 gaccgacgcc catatgccac cgcatcaaca cggcagccac cacggcgata cgcgattaat
    70081 tcgccatgct tatggtgaag gcgaaaagta tgtcccgttg gtcctccgcg cgcaaacgct
    70141 gtgggatgaa ctttcccgcc acaacgaaga tgatcccatt tttgtacgct ctggtgtcat
    70201 taacctcggc ccggctgact ccgcatttct cgccaacgtc gcccacagcg ccgaacagtg
    70261 gcaactcaac gttgaaaagc tcgatgcgca agggattatg gcccgctggc cagaaatacg
    70321 cgtcccggac aactacatcg gcttatttga gactgattcc ggttttttgc gcagcgaact
    70381 ggcgattaaa acctggatcc aactggcgaa ggaagcgggc tgtgcgcaac tgttcaactg
    70441 cccggtcacc gcaattcgtc atgacgatga tggcgtaact attgaaacgg ttgacggtga
    70501 gtatcaggcg aaaaaagcga ttgtctgcgc gggaacatgg gtaaaagacc tgctcccgga
    70561 gctgcctgtc cagcccgtac gtaaagtatt tgcctggtat caggccgatg gccgctatag
    70621 cgtgaagaat aaattcccgg cgtttaccgg tgaactgccc aatggcgatc aatattatgg
    70681 ttttccggca gaaaacgacg cgttgaagat tggcaaacat aacggaggcc aggttatcca
    70741 ttcagcggat gaacgtgttc cgtttgcgga agtggccagt gatgggtcag aagccttccc
    70801 gttcttgcgc aatgtattgc cgggtatcgg ttgctgcctg tacggcgctg cctgcaccta
    70861 tgataattcg cctgacgaag attttattat cgatacccta cccggccacg ataatacact
    70921 gctcattacc ggcctgagtg ggcacggttt taaatttgcg tcagttttag gggaaatagc
    70981 tgccgatttt gcgcaagacc aaaaaagcga ttttgatttg acgccattca ggctttcccg
    71041 cttccaataa tcatcattac ggcctccaga atgaggccgt ttttacagga gtatttaatg
    71101 cgtcggctgt tgcactatct catcaataat attcgcgagc atttgatgct ttatcttttt
    71161 ctctggggat tgttggcaat tatggatctg atctatgtgt tttattttta gttttccgca
    71221 ctgacataaa ctgacaaata tttaatctcc ctttcattta gcgtgaaaaa ttgaatgata
    71281 ttattcctgc ttctacagca ctcaccagac atctctgatg ctgaacccac taaacttatg
    71341 aagattcttt cattattaat tcaaaattat tgaaataaaa ataaatatca ttcaatttgt
    71401 tatttttgtg ttgcaattca ttctccgaca cgccattttc tttctctcgc aaattcgtat
    71461 tggatgcact tatgtcattc acaaataccc ctgaacgcta tggtgttata tctgcggcct
    71521 tccattggct aagcgcaatt attgtctacg gcatgtttgc tttaggtctg tggatggtca
    71581 cgctcagtta ttacgatggc tggtatcaca aagcacccga actgcataaa agcatcggta
    71641 ttttgttaat gatggggctg gttattcgcg ttctgtggcg tgtcatatct ccaccgccgg
    71701 gtccgctgcc gagttattcg cccatgactc gtcttgccgc aaaagctgga catctcgcat
    71761 tgtacctatt gctgttcgcc atcggcatca gcggctatct gatctcaact gccgatggta
    71821 aaccgatcag cgtttttggc tggtttgacg tccccgcgac ccttgccgac gctggcgcac
    71881 aggcagactt tgccggtgcc ctgcattttt ggctcgcgtg gagcgtcgta gtactgtctg
    71941 ttatgcacgg atttatggcc ctgaagcatc atttcatcga taaagacgac actctgaagc
    72001 gcatgctggg aaagtcgtca tctgactatg gagtataaaa aatgaaaaaa agcctgcttg
    72061 gtttaacctt cgcgtccctg atgttctctg ccggttcagc ggttgccgcc gattacaaaa
    72121 ttgacaaaga aggtcagcac gcctttgtta acttccgcat acagcatctt ggctatagct
    72181 ggttatatgg cacctttaaa gatttcgacg gtacttttac ctttgatgaa aaaaatccgg
    72241 ctgccgataa agtgaatgtg acaattaaca ccaccagcgt cgatactaac cacgccgaac
    72301 gcgataaaca tcttcgcagt gcggattttc tcaataccac aaaatatcca caggcaacat
    72361 tcacctccac cagcgtgaag aaagacggtg acgaactgga tattaccggc gatctgacgc
    72421 tgaatggcgt aaccaaaccc gtcacgctgg aagcgaaatt aattggtcag ggcgacgacc
    72481 catggggcgg taaacgtgca ggctttgagg ccgaaggcaa aattaagctc aaagacttca
    72541 atatcaagac agatttaggt ccagcttctc aggaagtgga tctgattatt tcagtggaag
    72601 gcgtacagca gaagtaagag tgaaatgttg ccggatggca cgcatccggc aatgatattt
    72661 attctgttgg atcaggaata cccagtgttg tattcagacg tccacgagac ttattaaaga
    72721 tcttattgcc attttcacgt cctgcccgac ggcgtcgctg ttcttctggc ggtaacgcgc
    72781 tttcttcgca gcaaatctca ctacaacaac ctttgtattt ttccgcgcat actggacact
    72841 gaataaacag caggtggcag ccatcatttt tacagttggt atggctgtcg cacggcgcgc
    72901 cgcactggtg gcaatgcgcg ataatctcat ccgatatacg ttcgcccatc cgctcgtcaa
    72961 aaacaaaatt tttgccaata aaacgcaccg gcaagccctg ctcgcgcgcc ttacgggcgt
    73021 attcaataat tccgccctcg atatgccaga ctttgttgaa tccgttatgt ttcatccagg
    73081 cactggcctt ttcacaacga atgccgccgg tgcagtacat gacgattttt ttatctttat
    73141 gtgcctgcat catctcgact gcttttggca gctgctcacg gaaggtatct gccggaattt
    73201 cgagcgcgtt ttcaaagtgc cccacttcat actcatagtg gttacgcatg tcgataaata
    73261 gtgcatcggg atcgtcaagc atggcgttca cttccgccgc ttgcagatac tcaccaacat
    73321 tgctggcatc aaagtgagga tcgtcaatac cgtcggcaac aatgcgatcg cgtaccttca
    73381 tgcgcagtac ccagaaggat ttcccgtcat catccaacgc gatattcagg cgtaaaccct
    73441 ctaaagccgg gtcgaaggca tagagctgcg cgcgaaatgt ttcaacattg ctcgcaggta
    73501 cgctgatttg cgcgttaatg ccctcatgcg ccagatacac tcgcccaaaa acattcagcg
    73561 cggtaaacag ctgatataaa gcgtcacggg tcgccttagg atcggcgatg tggaaatact
    73621 tgtaaaacga aatggtggtt cgcggttcgc tctcagccaa cattttggct tttaacgcgt
    73681 cgttggaaat gcggttgtgt aacactggca tggtgtacgg ttcctgcgag atgggaaagt
    73741 aaaaatccgc ggcatgatat agcaattatc gataattaac atccacacat tttacgctac
    73801 atttgcgcat taaaaattat ttgttattta caagcgcggc aatttcgccc agtcttcagc
    73861 cacaaatttt ggttgcgggc gaaaaaatgc gacaatacat acaattgccc gtataggttg
    73921 aaaaacagga ttgatatgac gaatctaccc aagttctcca ccgcactgct tcatccgcgt
    73981 tattggttaa cctggttggg tattggcgta ctttggttgg tcgtgcaatt gccctacccg
    74041 attatctacc gcctcggttg tggattagga aaactggcgt tacgttttat gaaacgacgc
    74101 gcaaaaattg tgcatcgcaa cctggaactg tgcttcccgg aaatgagcga acaagaacgc
    74161 cgtaaaatgg tggtgaagaa tttcgaatcc gttggcatgg gcctgatgga aaccggtatg
    74221 gcgtggttct ggccggaccg ccgaatcgcc cgctggacgg aagtgatcgg catggaacac
    74281 attcgtgacg tgcaggcgca aaaacgcggc atcctgttag ttggcatcca ttttctgaca
    74341 ctggagctgg gcgcgcggca gtttggtatg caggaaccgg ggattggcgt ttaccgcccg
    74401 aacgataatc cactgattga ctggctacaa acctggggcc gtttgcgctc aaataaatcg
    74461 atgctcgacc gcaaagattt aaaaggcatg attaaagccc tgaaaaaagg cgaagtggtc
    74521 tggtacgcac cagatcatga ttacggcccg cgctcaagcg ttttcgtccc gttatttgcc
    74581 gttgagcagg ctgcgaccac gaccggaacc tggatgctgg cacggatgtc cggcgcatgt
    74641 ctggtgccct tcgttccacg ccgtaagcca gatggcaaag ggtatcaatt gattatgctg
    74701 ccaccagagt gttctccgcc actggatgat gccgaaacta ccgccgcgtg gatgaacaaa
    74761 gtggtcgaaa aatgcatcat gatggcacca gagcagtata tgtggttaca ccgtcgcttt
    74821 aaaacacgcc cggaaggcgt tccttcacgc tattaaatct cccatgccgg atgcttcaga
    74881 atggcatccg gcattaccac agcaaatccc cctgatttag cgataaaagc tctctggatt
    74941 gcgccccctg gaagtcgggc gcataattag tgtgtttatc ttttcttctt atgttcaccg
    75001 cgcctggcgc accaacagcg gattgctatg tcaccctgtg aaaatgacac ccctataaac
    75061 tggaaacgaa acctgatcgt cgcctggcta ggctgttttc ttaccggggc cgccttcagt
    75121 ctggtaatgc ccttcttacc cctctacgtt gagcagcttg gtgttaccgg ccactccgcc
    75181 ctgaatatgt ggtccggtat tgtcttcagt ataatgggtt cctaaacaca tcattttagg
    75241 tattgaatat gagacgggca atcagctaca tcagattttc aagtgaacga caactgaaag
    75301 gggactctgt acgccgacaa tctaagctgg ttactgactg gcttgataag aaccctgagt
    75361 tttacttgga ttcatccttg agctttaaag atttggggaa aagtgctttt agtggtaagc
    75421 acctgaaagg tggtctaggt gactttttaa ccgctatcga gaaagggctt gttaaagctg
    75481 gtgatacgct actaatcgag agtttagacc gtctgtcacg tcaggatatt gatattgctt
    75541 ctgagctatt acgccgtatc ttaagggctg gtgtagatgt agtcacactg agcgatggag
    75601 aacattacac acgagaaagt ttaaaagacc cgttagccct gattaaaagc attctgataa
    75661 tgcagcgggc gcatgaggaa agtttgagaa agtctgagcg tgtccaagct gcatggaacc
    75721 gtaaaaaaga actcatctcc gaaggtatca aggtatct
//
LOCUS       DAJTQY010000004        75616 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.004, whole genome shotgun sequence.
ACCESSION   DAJTQY010000004 DAJTQY010000000
VERSION     DAJTQY010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 75616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 75616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..75616
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.004"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(435..641)
                     /locus_tag="OO349_000245"
     CDS             complement(435..641)
                     /locus_tag="OO349_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HCR5865462.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            954..1043
                     /gene="kdpF"
                     /locus_tag="OO349_000246"
     CDS             954..1043
                     /gene="kdpF"
                     /locus_tag="OO349_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HCR5865463.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            1043..2716
                     /gene="kdpA"
                     /locus_tag="OO349_000247"
     CDS             1043..2716
                     /gene="kdpA"
                     /locus_tag="OO349_000247"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HCR5865464.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGAQQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPSP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2739..4786
                     /gene="kdpB"
                     /locus_tag="OO349_000248"
                     /pseudo
     CDS             2739..4786
                     /gene="kdpB"
                     /locus_tag="OO349_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
     gene            4795..5367
                     /gene="kdpC"
                     /locus_tag="OO349_000249"
     CDS             4795..5367
                     /gene="kdpC"
                     /locus_tag="OO349_000249"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HCR5865465.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWYPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAAAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5360..8040
                     /gene="kdpD"
                     /locus_tag="OO349_000250"
                     /pseudo
     CDS             5360..8040
                     /gene="kdpD"
                     /locus_tag="OO349_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415223.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
     gene            8037..8714
                     /gene="kdpE"
                     /locus_tag="OO349_000251"
     CDS             8037..8714
                     /gene="kdpE"
                     /locus_tag="OO349_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HCR5865466.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVFEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSPVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSGVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFML"
     gene            8989..9093
                     /locus_tag="OO349_000252"
     CDS             8989..9093
                     /locus_tag="OO349_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2618 domain-containing protein"
                     /protein_id="HCR5865467.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            9404..11601
                     /gene="speF"
                     /locus_tag="OO349_000253"
                     /pseudo
     CDS             9404..11601
                     /gene="speF"
                     /locus_tag="OO349_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
     gene            11598..12917
                     /gene="potE"
                     /locus_tag="OO349_000254"
     CDS             11598..12917
                     /gene="potE"
                     /locus_tag="OO349_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HCR5865468.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFIANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGGIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(12973..14613)
                     /gene="pgm"
                     /locus_tag="OO349_000255"
     CDS             complement(12973..14613)
                     /gene="pgm"
                     /locus_tag="OO349_000255"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HCR5865469.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGYVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(14639..15184)
                     /gene="seqA"
                     /locus_tag="OO349_000256"
     CDS             complement(14639..15184)
                     /gene="seqA"
                     /locus_tag="OO349_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HCR5865470.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            15369..16133
                     /gene="ybfF"
                     /locus_tag="OO349_000257"
     CDS             15369..16133
                     /gene="ybfF"
                     /locus_tag="OO349_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR5865471.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPREPVMNYPAMAQDLVDTLDALQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            16273..16566
                     /gene="ybfE"
                     /locus_tag="OO349_000258"
     CDS             16273..16566
                     /gene="ybfE"
                     /locus_tag="OO349_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HCR5865472.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            16706..17236
                     /gene="fldA"
                     /locus_tag="OO349_000259"
     CDS             16706..17236
                     /gene="fldA"
                     /locus_tag="OO349_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HCR5865473.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            17446..17532
                     /gene="uof"
                     /locus_tag="OO349_000260"
     CDS             17446..17532
                     /gene="uof"
                     /locus_tag="OO349_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HCR5865474.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            17525..17971
                     /gene="fur"
                     /locus_tag="OO349_000261"
     CDS             17525..17971
                     /gene="fur"
                     /locus_tag="OO349_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HCR5865475.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(18055..18381)
                     /locus_tag="OO349_000262"
     CDS             complement(18055..18381)
                     /locus_tag="OO349_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5865476.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(18418..19824)
                     /gene="chiP"
                     /locus_tag="OO349_000263"
     CDS             complement(18418..19824)
                     /gene="chiP"
                     /locus_tag="OO349_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HCR5865477.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSIGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWIKADSQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFSAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
     gene            complement(20403..22067)
                     /gene="glnS"
                     /locus_tag="OO349_000264"
     CDS             complement(20403..22067)
                     /gene="glnS"
                     /locus_tag="OO349_000264"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HCR5865478.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHITVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(22270..24216)
                     /gene="nagE"
                     /locus_tag="OO349_000265"
     CDS             complement(22270..24216)
                     /gene="nagE"
                     /locus_tag="OO349_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HCR5865479.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVIFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLLIKAQGHVVAGQTPLYEIKK"
     gene            24549..25349
                     /gene="nagB"
                     /locus_tag="OO349_000266"
     CDS             24549..25349
                     /gene="nagB"
                     /locus_tag="OO349_000266"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCR5865480.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMIMVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            25409..26557
                     /gene="nagA"
                     /locus_tag="OO349_000267"
     CDS             25409..26557
                     /gene="nagA"
                     /locus_tag="OO349_000267"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCR5865481.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIDVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            26566..27786
                     /gene="nagC"
                     /locus_tag="OO349_000268"
                     /gene_synonym="nagR"
     CDS             26566..27786
                     /gene="nagC"
                     /locus_tag="OO349_000268"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HCR5865482.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            27834..28586
                     /gene="nagD"
                     /locus_tag="OO349_000269"
     CDS             27834..28586
                     /gene="nagD"
                     /locus_tag="OO349_000269"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HCR5865483.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            28983..30647
                     /gene="asnB"
                     /locus_tag="OO349_000270"
     CDS             28983..30647
                     /gene="asnB"
                     /locus_tag="OO349_000270"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HCR5865484.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKNHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            31026..31102
                     /locus_tag="OO349_000271"
     tRNA            31026..31102
                     /locus_tag="OO349_000271"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31060..31062,aa:Met,seq:cat)
     gene            31111..31195
                     /locus_tag="OO349_000272"
     tRNA            31111..31195
                     /locus_tag="OO349_000272"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31145..31147,aa:Leu,seq:tag)
     gene            31219..31293
                     /locus_tag="OO349_000273"
     tRNA            31219..31293
                     /locus_tag="OO349_000273"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31251..31253,aa:Gln,seq:ttg)
     gene            31328..31402
                     /locus_tag="OO349_000274"
     tRNA            31328..31402
                     /locus_tag="OO349_000274"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31360..31362,aa:Gln,seq:ttg)
     gene            31418..31494
                     /locus_tag="OO349_000275"
     tRNA            31418..31494
                     /locus_tag="OO349_000275"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31452..31454,aa:Met,seq:cat)
     gene            31543..31617
                     /locus_tag="OO349_000276"
     tRNA            31543..31617
                     /locus_tag="OO349_000276"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31575..31577,aa:Gln,seq:ctg)
     gene            31655..31729
                     /locus_tag="OO349_000277"
     tRNA            31655..31729
                     /locus_tag="OO349_000277"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31687..31689,aa:Gln,seq:ctg)
     gene            complement(31883..33058)
                     /gene="ubiF"
                     /locus_tag="OO349_000278"
     CDS             complement(31883..33058)
                     /gene="ubiF"
                     /locus_tag="OO349_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HCR5865485.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            33204..34628
                     /gene="miaB"
                     /locus_tag="OO349_000279"
     CDS             33204..34628
                     /gene="miaB"
                     /locus_tag="OO349_000279"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HCR5865486.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRM
                     AETPESVIARTRKENDLGVGYYQP"
     gene            34781..35821
                     /locus_tag="OO349_000280"
     CDS             34781..35821
                     /locus_tag="OO349_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HCR5865487.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            35818..36285
                     /gene="ybeY"
                     /locus_tag="OO349_000281"
     CDS             35818..36285
                     /gene="ybeY"
                     /locus_tag="OO349_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HCR5865488.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            36375..37253
                     /gene="corC"
                     /locus_tag="OO349_000282"
     CDS             36375..37253
                     /gene="corC"
                     /locus_tag="OO349_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HCR5865489.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            37278..38816
                     /gene="lnt"
                     /locus_tag="OO349_000283"
     CDS             37278..38816
                     /gene="lnt"
                     /locus_tag="OO349_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HCR5865490.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIATPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPLMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIIL
                     AEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFAREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     QRRK"
     gene            39213..40121
                     /gene="gltI"
                     /locus_tag="OO349_000284"
     CDS             39213..40121
                     /gene="gltI"
                     /locus_tag="OO349_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HCR5865491.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            40291..41031
                     /gene="gltJ"
                     /locus_tag="OO349_000285"
     CDS             40291..41031
                     /gene="gltJ"
                     /locus_tag="OO349_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HCR5865492.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWVWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQTYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAFIMLVMTLVERKVRLPGNMGGK"
     gene            41031..41705
                     /gene="gltK"
                     /locus_tag="OO349_000286"
     CDS             41031..41705
                     /gene="gltK"
                     /locus_tag="OO349_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HCR5865493.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVSWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            41705..42430
                     /gene="gltL"
                     /locus_tag="OO349_000287"
     CDS             41705..42430
                     /gene="gltL"
                     /locus_tag="OO349_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HCR5865494.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMAPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            42548..43483
                     /gene="rihA"
                     /locus_tag="OO349_000288"
     CDS             42548..43483
                     /gene="rihA"
                     /locus_tag="OO349_000288"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HCR5865495.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVIVDVDRQGFVDLLADRLKFYA"
     gene            43567..45228
                     /gene="hscC"
                     /locus_tag="OO349_000289"
                     /pseudo
     CDS             43567..45228
                     /gene="hscC"
                     /locus_tag="OO349_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
     gene            complement(45288..46739)
                     /gene="djlC"
                     /locus_tag="OO349_000290"
     CDS             complement(45288..46739)
                     /gene="djlC"
                     /locus_tag="OO349_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HCR5865496.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDATAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMVELIPVAKQWQAEEPESEDARYYQCAQRLYCGEGESLLADLGA
                     YWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(46736..47443)
                     /locus_tag="OO349_000291"
     CDS             complement(46736..47443)
                     /locus_tag="OO349_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR5865497.1"
                     /translation="MNKEEQYLLFALSAPMEILNQSCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWQRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRAGVLNNWLTEEESLWLQSRVYARAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
     gene            47545..48099
                     /locus_tag="OO349_000292"
     CDS             47545..48099
                     /locus_tag="OO349_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCR5865498.1"
                     /translation="MNKKLMYIFAIFIVAAITCISQPKKTMLRDKAMVNYAFDYLRSP
                     GSLPLTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLRWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(48109..49536)
                     /locus_tag="OO349_000293"
     CDS             complement(48109..49536)
                     /locus_tag="OO349_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HCR5865499.1"
                     /translation="MKNCWKILDIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLSSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSASRLGIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCY
                     YWREYPSTQADALMLQWCRQHRVYYYPLVVMMIEARDLVNDQGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHEGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(49533..50240)
                     /locus_tag="OO349_000294"
     CDS             complement(49533..50240)
                     /locus_tag="OO349_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR5865500.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRIIHRMIENGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LADYPTCPETLKDMSDL"
     gene            50404..51381
                     /locus_tag="OO349_000295"
     CDS             50404..51381
                     /locus_tag="OO349_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HCR5865501.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWILDRGEDPNYKEAVTWYQIAAESGMSYAQNNLGWMYRNGNGVAQDYTLAFFWYK
                     QAALQGHSYAQDNLADLYKDGEGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEALL"
     gene            complement(51451..51933)
                     /locus_tag="OO349_000296"
     CDS             complement(51451..51933)
                     /locus_tag="OO349_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HCR5865502.1"
                     /translation="MNKVAQYYRELVASLNERLRNGERDIDALVEQARERVIKTGELT
                     RTEIDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EIFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGYDQFQRRPFEP
                     "
     gene            52168..54750
                     /gene="leuS"
                     /locus_tag="OO349_000297"
     CDS             52168..54750
                     /gene="leuS"
                     /locus_tag="OO349_000297"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HCR5865503.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALQAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            54765..55346
                     /gene="lptE"
                     /locus_tag="OO349_000298"
     CDS             54765..55346
                     /gene="lptE"
                     /locus_tag="OO349_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HCR5865504.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTMLGN"
     gene            55346..56377
                     /gene="holA"
                     /locus_tag="OO349_000299"
     CDS             55346..56377
                     /gene="holA"
                     /locus_tag="OO349_000299"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HCR5865505.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAVRAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            56379..57020
                     /gene="nadD"
                     /locus_tag="OO349_000300"
     CDS             56379..57020
                     /gene="nadD"
                     /locus_tag="OO349_000300"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HCR5865506.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            57044..57655
                     /locus_tag="OO349_000301"
     CDS             57044..57655
                     /locus_tag="OO349_000301"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HCR5865507.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARLVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            57915..58232
                     /gene="rsfS"
                     /locus_tag="OO349_000302"
                     /gene_synonym="rsfA"
     CDS             57915..58232
                     /gene="rsfS"
                     /locus_tag="OO349_000302"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HCR5865508.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            58236..58703
                     /gene="rlmH"
                     /locus_tag="OO349_000303"
     CDS             58236..58703
                     /gene="rlmH"
                     /locus_tag="OO349_000303"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HCR5865509.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            58734..60635
                     /gene="mrdA"
                     /locus_tag="OO349_000304"
     CDS             58734..60635
                     /gene="mrdA"
                     /locus_tag="OO349_000304"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HCR5865510.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     EPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTANKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            60638..61750
                     /gene="mrdB"
                     /locus_tag="OO349_000305"
                     /gene_synonym="rodA"
     CDS             60638..61750
                     /gene="mrdB"
                     /locus_tag="OO349_000305"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HCR5865511.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            61761..62849
                     /gene="rlpA"
                     /locus_tag="OO349_000306"
     CDS             61761..62849
                     /gene="rlpA"
                     /locus_tag="OO349_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HCR5865512.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTKLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPSVLEGSEPTPAPQPVVTASSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            62988..64199
                     /gene="dacA"
                     /locus_tag="OO349_000307"
     CDS             62988..64199
                     /gene="dacA"
                     /locus_tag="OO349_000307"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HCR5865513.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFSDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            64310..64573
                     /locus_tag="OO349_000308"
     CDS             64310..64573
                     /locus_tag="OO349_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HCR5865514.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            64674..65315
                     /gene="lipB"
                     /locus_tag="OO349_000309"
     CDS             64674..65315
                     /gene="lipB"
                     /locus_tag="OO349_000309"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HCR5865515.1"
                     /translation="MYQDKILVRQLGLQPYEPISRAMHEFTDTRDNSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            65574..66527
                     /locus_tag="OO349_000310"
     CDS             65574..66527
                     /locus_tag="OO349_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HCR5865516.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICRKGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFILRDPILHGVIDVIRNAF"
     gene            66736..67701
                     /gene="lipA"
                     /locus_tag="OO349_000311"
     CDS             66736..67701
                     /gene="lipA"
                     /locus_tag="OO349_000311"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HCR5865517.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(67802..68005)
                     /gene="tatE"
                     /locus_tag="OO349_000312"
     CDS             complement(67802..68005)
                     /gene="tatE"
                     /locus_tag="OO349_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HCR5865518.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(68134..68922)
                     /locus_tag="OO349_000313"
     CDS             complement(68134..68922)
                     /locus_tag="OO349_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706584.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HCR5865519.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHLPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRHVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPILNNRRFAPPQLL"
     gene            69015..69398
                     /gene="crcB"
                     /locus_tag="OO349_000314"
     CDS             69015..69398
                     /gene="crcB"
                     /locus_tag="OO349_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HCR5865520.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTVH"
     gene            complement(69452..69661)
                     /gene="cspE"
                     /locus_tag="OO349_000315"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(69452..69661)
                     /gene="cspE"
                     /locus_tag="OO349_000315"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCR5865521.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(69836..70396)
                     /gene="pagP"
                     /locus_tag="OO349_000316"
     CDS             complement(69836..70396)
                     /gene="pagP"
                     /locus_tag="OO349_000316"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HCR5865522.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADERMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
     gene            70984..72369
                     /gene="dcuC"
                     /locus_tag="OO349_000317"
     CDS             70984..72369
                     /gene="dcuC"
                     /locus_tag="OO349_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HCR5865523.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLDRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTDK"
     gene            complement(72410..72751)
                     /locus_tag="OO349_000318"
                     /pseudo
     CDS             complement(72410..72751)
                     /locus_tag="OO349_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(73089..73598)
                     /locus_tag="OO349_000319"
     CDS             complement(73089..73598)
                     /locus_tag="OO349_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR5865524.1"
                     /translation="MKSANEINQWMTPKQITELDGMPGTIQGVHKRAKKEGWPKRSQE
                     GRRGPGVEYIPAIPAMQKIAIEEMNADTLKMFSLLINKLGENEVREIELNMAKYGLSG
                     LMQERPSISVDTLLATLGIDRQTLQTALALHKLPPETRQEILSMYGVHKQEEPVAPLL
                     EPQDAKKAV"
     gene            73761..74006
                     /locus_tag="OO349_000320"
     CDS             73761..74006
                     /locus_tag="OO349_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013024337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5865525.1"
                     /translation="MRKMSNDWHRADVIAALKKTGTPLAKLSRRSGYSTGTLANALVR
                     PWPKGERIIADALNMKPEEIWPSRYLRDHNERVDISN"
     gene            73981..75612
                     /locus_tag="OO349_000321"
                     /pseudo
     CDS             73981..75612
                     /locus_tag="OO349_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683974.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
BASE COUNT        18610 a        18990 c        19205 g        18811 t
ORIGIN      
        1 cataacatct tattggatta gatatgttaa caattaatta ttcttaatat atcaataaag
       61 tatatattac tctctttact atctgttttt attgcggtag cgatactaat aacaaaattc
      121 ggaatttatt tagatgctgg aattagaaaa agggatgacg gctttctcac cttaccctga
      181 agtttcacga aagtgtgtaa ggaagtatgc aatctaactg gagatttctg atgatctagt
      241 cgcttagagc gacaatgaag gtgaccgcat cctgggtagc cctatgaaag gatactattg
      301 aaacgtgatg ctaaaacagt gatgaaatga ataatacgat ggaaacgtct tccatgttaa
      361 ttatcatgga agacgttgat gttgaacacc taaaccagcc ccctgatacg aggctggctt
      421 aagcgcgcag gcaatcagta aaaatcaccg gttgcctttt cggcctgatc catccacacc
      481 ggtttatcgc tggttttcga ccagacgcgg tgcaggtagc tgtaaaagcg ggcgcggtct
      541 ttccagaaaa gcatgaaagg cagcgccaga acgcccgctg caaccgcata tgtacggcgt
      601 aaaaagataa gccatgcagg atattctctg tagagttcca tacttttctc ccccttttgt
      661 atctacccgg tgaatagcac cggaaaaatg aatttgttta tctgatgaaa atagtaccgc
      721 cttttgtgta attttactac tcatccgacc acttattttt gcttattgat ggtttattta
      781 cattcacact gtaattaaat tacacaaaag ttaaattaat actaaccatc agttaaatca
      841 tggcctttgc catttttata ctttttttac accccgtccg cagatttttg cgaaatcttt
      901 gcagccagaa ttctactctt ccggtatcac ttttaggcca ctggaggtgc actgtgagtg
      961 caggcgtgat agccggcgtg ttgctggtgt ttttattact gggttatttg gtttatgccc
     1021 tgatcaatgc ggaggcgttc tgatggctgc gcaagggttc ttactgattg ccacgttttt
     1081 actggtatta atggtgctgg cgcgtccttt aggcagcggg ctggcgcggc tgattaatga
     1141 cattcctctt ccaggtacag cgggcgttga acgtatactc tttcgtttac ctggcgtctc
     1201 tgaccatgag atgaactgga agcaatatct ttgtgccatt ctcggcctga acatgctggg
     1261 gctggcggtg ctgtttttta tgttgctcgg tcagcactat ctgccgctta atccacagca
     1321 gttgccgggg ctgtcgtggg atctggcgct gaataccgcc gtcagctttg tcacgaatac
     1381 caactggcaa tcttatagcg gtgaaaccac gttgagctat ttcagccaga tggcaggctt
     1441 aacggtacaa aactttcttt ctgccgccag cgggattgcg gtgatttttg ccttcatccg
     1501 tgcgtttact cgccagagca tgagcacact cgggaatgcc tgggtcgatc tgctacgcat
     1561 cacgttatgg gtgctagtcc ctgtggcgtt gttgatcgca ctgtttttta ttcaacaagg
     1621 tgcgcagcaa aactttctgc cctatcaggc agtgaatacc gttgaaggag cgcaacagct
     1681 gttacccatg ggaccagtag cttctcagga agcgatcaag atgctcggta ctaacggcgg
     1741 tggcttcttt aatgccaact cgtcgcatcc gtttgaaaac ccaaccgcac tgaccaactt
     1801 cgtgcagatg ctggcgatct tcttgatccc aacagcgctg tgctttgcct ttggtgaagt
     1861 gacgggcgat cgccgccagg ggcgcatgtt gctgtgggcg atgtcagtga tttttgtcat
     1921 ctgcgtaggc gtggttatgt gggcagaagt tcagggtaat cctcatctgc tggcgctcgg
     1981 cgcagacagc agcatcaata tggaaggtaa agagagccgt ttcggcgtgc tggtcagtag
     2041 cctgtttgcg gtcgtgacta cggcggcgtc ctgtggcgcg gtgattgcga tgcatgattc
     2101 gtttaccgct ctcggcggca tggtgccgat gtggctaatg caaattggtg aagtggtgtt
     2161 cggcggtgtc ggttctggtc tttacggcat gatgctgttt gtcctgctgg cggtgtttat
     2221 tgccgggctg atgattggtc gtacaccgga atatctgggt aaaaaaatag acgtacgcga
     2281 gatgaaactg actgcactgg cgattctggt tactccaaca ctggtactga tgggcgcggc
     2341 gttggcgatg atgaccgacg ccggacgtag cgccatgctt aaccctagcc cgcatggttt
     2401 tagcgaagtg ctgtacgccg tgtcgtccgc cgctaacaac aacggcagcg cctttgccgg
     2461 attaagcgcc aactctccgt tctggaactg tttactggcg ttctgcatgt ttgtcggtcg
     2521 cttcggggtg attatcccgg tgatggcaat tgccggttcg ctggtgagta aaaagagcca
     2581 acccgccagc tccggcacgc tgccaacgca cggcccgctg tttgttggcc tgttaatcgg
     2641 caccgtgttg ctggttggcg cactgacctt tatccctgcc ctggcgcttg gtccggtggc
     2701 ggaatatctc tcctgatgat attgagtgag cactgaatat gagtcgtaaa caactggcgc
     2761 tattcgaacc aacacttgtc gttcaggcgc tgaaagaagc ggtgaaaaaa ttaaacccgc
     2821 aggcgcaatg gcgcaatccg gtgatgttta tcgtctggat cggcagtctg ctgaccacct
     2881 gtattagcat cgcgatggca agcggtgcga tgcctggcaa tgcgctgttt agcgcggcaa
     2941 ttagcggctg gctgtgggtc accgtactgt tcgctaattt cgccgaggcg ctggcagaag
     3001 gccgcagtaa agcgcaggcc aacagtctta aaggggtgaa aaaaactgcc tttgcccgca
     3061 agctgcgtga gccgaaatat ggcgctgcgg cggacaaagt tcctgccgat caactgcgta
     3121 aaggtgacat cgtgctggta gaagctggcg atattatccc ctgcgatggt gaagtcattg
     3181 aaggcggcgc atcggtcgat gaaagcgcca tcaccgggga atcggcaccg gtgatccgtg
     3241 aatccggcgg cgattttgcc tccgtcaccg gcggcacgcg tattctttct gactggctgg
     3301 tgattgagtg tagcgttaac cccggtgaga catttctgga tcggatgatc gcgatggtgg
     3361 aaggcgcaca gcgacgcaaa acaccgaacg agattgccct gactatcctg ctgattgctc
     3421 tgaccatcgt ctttttactg gcaaccgcca cgctgtggcc gttttccgcg tggggcggta
     3481 atgccgtgag tgtaacggta ctggtggcgc tgctggtctg tctgatccca accactattg
     3541 gtggcctgtt gtcagcgatc ggcgtcgccg ggatgagtcg gatgctgggc gcgaatgtga
     3601 ttgccaccag cggacgtgca gttgaagcgg caggtgacgt tgacgttctg ctactggata
     3661 aaaccggcac catcacactc ggtaaccgtc aggcgtcgga gtttatcccc gcgcagggcg
     3721 tggatgaaaa aacgctggct gacgccgcac aactggcttc gctggctgat gaaacgccgg
     3781 aaggtcgcag tattgtgatc ctcgccaagc aacgttttaa cctgcgcgag cgcgatgtgc
     3841 agtcgctcca tgccaccttt gtaccgttta ctgcgcaaag ccggatgagc gggatcaaca
     3901 tcgacaaccg catgatccgt aaaggttctg tcgatgccat tcgccgccat attgaggcta
     3961 acggcggtca cttccctgcc gatgttgatc aaaaagtcga tcaggttgcg cgtcaaggag
     4021 ccacgctgct ggtggtggtg gaaggttctc gtgtgctggg cgttattgcg ctgaaagata
     4081 tcgtcaaagg cggtattaaa gagcgcttcg cccagctgcg caaatgggca ttaaaacggt
     4141 gatgattacc ggcgataacc gtctgactgc cgccgcgata gctgcggaag cgggtgtcga
     4201 tgattttctc gccgaagcga caccggaggc caagctggca ttgatccgtc agtatcaggc
     4261 ggaaggtcgt ttggtagcga tgaccggcga cagcaccaac gatgctacgg cgctggcgca
     4321 ggcagatgtc gcggtggcga tgaactccgg cacccaggcg gctaaagagg cgggcaatat
     4381 ggtggatctc gactctaacc cgaccaagtt gatcgaggtg gtgcacattg gcaaacagat
     4441 gctgatgacc cgtggctcgc tgaccacctt cagcattgcc aacgatgtgg cgaaatactt
     4501 cgccattatt ccggcggcgt tcgcggcaac atatccgcag ttaaatgcgc tgaacatcat
     4561 gcgcctgcat tcgcccgact ccgccattct cagtgcggtc attttcaacg ccttgattat
     4621 cgtctttttg attcctctgg cgttaaaagg cgtgagttat aaaccgctta ccgcttctgc
     4681 catgttgcgc cgtaacttat ggatttacgg tctgggtggg ctgctggtgc cgtttattgg
     4741 tatcaaagtc attgatttac tgctaaccgt ttgtagtctg gtgtgaggtt taccatgagt
     4801 ggattacgtc cggcattatc gacatttctc tttctgttat tgattactgg cggcgtttac
     4861 ccgctgctga ccaccgcact ggggcaatgg tggtatccct ggcaggccaa cggttcgttg
     4921 attcgtgaag gtgatacggt gcgcggttcg gcattaatcg ggcaaaattt taccggcaac
     4981 ggctattttc atggtcgccc gtcggcaaca gcagaaatgc cctataatcc acaggcttct
     5041 ggcgggagca atctggcagt cagtaacccg gagctggata aacagatagc cgcacgcgtt
     5101 gccgcattac gggccgctaa cccggacgcc agcacgaacg ttccggttga actggtgacg
     5161 gcgtcggcaa gcggactgga caataacatc accccgcaag cggcggcctg gcagatccca
     5221 cgcatcgcaa aagcgcgtaa tctcagcgtt gaacagctca cgcagctgat cgcaaaatac
     5281 agccaacaac cgctggtgaa atatatcggc cagccggtcg tcaacattgt cgaactcaat
     5341 ctggcactgg ataagcttga tgaataacga acccttacgt cccgaccccg accgtctgct
     5401 ggaacaaact gccgcgccgc atcgggggaa gctgaaagtt ttcttcagtg cctgtgcagg
     5461 cgtcgggaag acctgggcga tgctggcaga agcccagcga ctgcgggcgc aagggctgga
     5521 tattgtggtt ggtgtggtag aaacccatgg gcggaaagat accgccgcca tgcttgaagg
     5581 gctggctgtt ctgcctctga aacgccaggc gtaccgtggg cggcatatca gcgagtttga
     5641 tctcgatgcc gccctcgccc gccgtccggc gctgatctta atggacgaac tggcgcacag
     5701 taatgcgcca ggttctcgtc atcctaaacg ctggcaggat atcgaagaac tgctggaagc
     5761 tggcattgat gttttcacaa ccgtcaacgt tcagcatctg gaaagtctga atgatgtggt
     5821 cagcggcgtc accggaattc aggtacggga aaccgtgccc gatccttttt tcgatgccgc
     5881 cgacgacgtg gtgctggtgg acttgccccc ggacgatctg cgccagcggc tgaaagaagg
     5941 caaagtctat attgccgggc aggcggagcg cgccattgaa cattttttcc gcaaaggcaa
     6001 tctgatcgcc ctgcgcgaac tggcactgcg ccgtactgcc gatcgcgttg atgagcaaat
     6061 gcgcgcctgg cgggggcatc ctggcgaaga gaaagtctgg cacacgcgcg acgcgatcct
     6121 tttatgcatc ggccataaca ccggcagcga aaactggtcc gcgcggcggc gcggctgacg
     6181 tcacggctgg ggagcgtctg gcacgcggtg tatgttgaaa cccctgccct gcatcgctta
     6241 ccggagaaaa aacgtcgggc aattctcagc gccttacgtc tggcgcagga gctgggcgcg
     6301 gagacggcaa cactttctga tccagcggaa gagaaagcgg tagtgcgtta tgcccgtgaa
     6361 cataatctcg gcaagattat tctcggtcgc ccagcctcgc gccgctggtg gcgtcgggaa
     6421 acatttgctg accgactggc gcgcatcgcc cccgatctcg atcaggtgct ggtcgcgctt
     6481 gatgaaccac ccgcccgcac gattaacaac gcgccggata gccgctcatt taaagacaag
     6541 tggcgggtac agattcaggg atgcgtggtt gccgccgcgt tatgcgccgt tatcacctta
     6601 attgccatgc agtggctgat ggcgtttgat gccgccaacc tggtgatgct gtatctgctt
     6661 ggcgtggtgg tggcgctatt ttatggacgc tggccttcgg tggttgccac cgtcattaat
     6721 gtggtgagtt tcgatctctt ttttatcgcc ccacgcggca cgctcgccgt ctctgatgtg
     6781 caatatctgc tgaccttcgc ggtgatgtta accgtcgggc tggtgatcgg gaaccttact
     6841 gctggcgtgc gttatcaggc gcgggtagcc cgttaccgcg agcaacgcac acggcactta
     6901 tatgaaatgt cgaaagctct ggcggtgggc cgcagtccgc aggacatcgc cgccaccagc
     6961 gaacaattta ttgcctccac gtttcatgcc cgcagtcagg tgttgttgcc cgatgacaac
     7021 ggtaaattgc agccgttaac acatccgcaa ggagttacgc catgggacga tgccatcgcg
     7081 cagtggagtt ttgataaagg cctgcctgcg ggcgcgggca ccgacacgtt acccggtgta
     7141 ccgtaccaga ttttgccgct aaaaagcggc gagaaaacct acgggctggt ggtggtggag
     7201 ccggggaatc tgcgccagtt gatgatcccg gaacagcagc gcctgctgga gacttttacg
     7261 ctgttagtcg ccaatgccct tgagcggctg acgctaaccg ccagcgaaga acaggcgcgg
     7321 atggcaagcg aacgtgaaca gatccgcaac gccctgctgg cagcgctttc gcatgattta
     7381 cgcacgccgc ttacggtgct gtttggtcag gcagaaatct taacgctcga tctggcaagc
     7441 gaaggatcac cccacgcccg ccaggccagc gagattcgtc agcatgtgct gaacactacc
     7501 cgactggtga ataatctact ggatatggcg cgaattcagt ccggcggctt taatttgaag
     7561 aaagagtggt taacgctgga agaagtagtc ggcagcgcgc tgcaaatgcc ggaaccgggt
     7621 ttatcgtcgc ccatcaatct ttctctgcca gaaccgctga ccttaatcca cgttgacggg
     7681 ctactctttg aacgggtgct gattaatctg ctggagaacg cggtgaaata tgcgggtgcg
     7741 caggccgaaa ttggtatcga tgcccacgtt gagggcgaaa atctacaact ggatgtctgg
     7801 gataacggcc ccggtcttcc gccaggccag gagcagacga tatttgataa gtttgctcgc
     7861 gggaataaag agtcggcggt gccgggtgtc gggcttgggt tggcaatttg tcgggcgatt
     7921 gtggatgtac acggcggcac cattaccgcg ttcaaccgac cggaaggtgg tgcctgtttt
     7981 cgtgttacac ttccccagca aaccgctcct gaacttgaag actttcatga ggatatgtga
     8041 caaacgttct gattgttgaa gatgaacagg ctattcgtcg ctttctgcgc acggcgctgg
     8101 agggcgacgg gatgcgcgtc tttgaggccg aaactctgca acgcggcttg ctggaagcgg
     8161 caacccgtaa gccagatttg attattctcg atctcggcct gcccgatggt gatgggattg
     8221 agtttatccg cgacctgcgc cagtggagcc cggtgccggt gattgtgctt tccgcacgca
     8281 gcgaagagag cgacaagatc gccgcgctgg atgccggagc ggatgattat ctgagtaagc
     8341 cgtttggtat tggcgaattg caggcccgtc tgcgcgtcgc tttacgccgc cactccgcca
     8401 ccgccgcgcc cgatccgctg gtgaaatttt ccggtgttac cgtcgattta gccgcccgcg
     8461 tgattcaccg gggtgaggaa gaggtgcatc tcacaccaat tgagttccgc ctgctggcgg
     8521 tgctgctcaa caatgccgga aaagtactca cccagcgcca gctccttaac caggtgtggg
     8581 ggccaaacgc ggtcgaacac agtcactatt tgcgtattta tatgggacat ctgcgacaaa
     8641 aactggaaca ggatccggcc cggcctcgcc acttcattac tgaaaccggt attgggtatc
     8701 ggtttatgct ttgaattgtt attcaataaa ttcgcttaaa tagtgattaa tttattatta
     8761 aacctgcgcc gaatattatt atttggcagg tttaatttct taacgcaaat ataaaacacg
     8821 aaattacaac atttaagtaa caaaacagca tctaatatgg cgatattcat aattaatgat
     8881 ttattatctg gggtatgatc gttttttgtt gatcttcttc acagattata gccatttcat
     8941 ggatagaata actctacctt caactgacac agcaagcggt aaaggtaaat ggaaaataat
     9001 aaccgcttaa tgcctcatat aaggcggaca acccatatca tgaagtttgc ccatcgtaat
     9061 agttttgact ttcatttctt taatgcccgc tagtcttctg actaaagggc accccaacgt
     9121 acaggtctcc ctgactttaa gcattacagg ttaatacctg tattcctcgg tgctcatata
     9181 ctgctaaccc ttttaaactc taaataattc gagtcgcagc acttgcaact tgaggtatga
     9241 cgagtatagc cagttaccgg gctggtctgg gttattgcat ctgcaaaaag caaactactg
     9301 atttatttat cagcggtgga gctttgcttt ttttccgacg tgatcgattt ctcctttgag
     9361 aaattgagga cctgctatta cctgaaataa agagatgaat aaaatgtcag aattaaaaat
     9421 tgccgttagc cgttcttgcc cggattgttt ttccactcat cgtgcatgcg tgaatattga
     9481 cgaaagtaat tatattgacg ttgccgccat tattttatca gtcaatgatg ttgaacgtgg
     9541 aaaactcgat aaaatagacg ctactggcta tggcattcct gtttttattg caacggaaaa
     9601 tgaagaacgt gtaccagcag aatatctgcc acgtatttct ggtgtcttcg agcattgcga
     9661 atcacggaaa gagttttatg gacgtcagtt agaaaccgct gctagccatt atgaaacgca
     9721 actgcgcccg cccttcttcc gcgcactggt cgattatgtc aatcagggca atagcgcttt
     9781 tgactgcccg ggtcatcagg gaggtgagtt tttccgtcgt catccggcag ggaatcagtt
     9841 cgtggagtac ttcggagaaa cgctgttccg ttccgactta tgcaacgcag acgtagcaat
     9901 gggcgatctg ctgattcacg aaggcgcgcc atgcattgca cagcaacatg cggcaaaagt
     9961 gtttaatgcc gataaaacat acttcgttct aaatggcact tcatcttcta acaaagtggt
    10021 tttaaacgcc ctgctaacac cgggtgatct ggtgctgttt gatcgcaata accacaaatc
    10081 taaccaccac ggagcgttgc tacaggctgg tgcaacaccg gtttatctgg aaacggcacg
    10141 taacccgtat ggctttatcg gtggcattga tgcgcactgt tttgaagaaa gttacctgcg
    10201 tgagctgatc gcggaagtcg caccgcagcg ggcaaaagag gctcgtcctt tccgcctcgc
    10261 tgtgattcag ttaggcacct acgacggtac gatttataac gcccgccagg tggtggataa
    10321 aattggtcat ctgtgtgact acattctgtt tgactccgca tgggttggct atgagcaatt
    10381 tatcccgatg atggctgact gttcgccgct gttgctggat cttaatgaga acgatccggg
    10441 tattctagtt acgcaatccg tacataaaca gcaggctggt ttttctcaaa cttcacaaat
    10501 ccataaaaaa gacagccaca tcaaagggca acagcgttat gtaccgcaca aacgcatgaa
    10561 caacgccttt atgatgcacg cctccaccag cccgttctat ccgctgtttg ccgcactgga
    10621 tatcaacgcc aaaatgcatg aaggtgtcag cggtcgtaat atgtggatgg attgtgtggt
    10681 aaatggcatt aatgcccgca aactgatcct cgataactgt cagcatattc gtccgttcgt
    10741 acctgaactg gtggatggta aaccctggca gtcgtatgaa acagcgcaaa ttgcggttga
    10801 tctgcgcttc ttccagtttg taccagggga acactggcat tcttttgaag gctatgcaga
    10861 gaatcaatac tttgtcgatc cttgcaaact gttgctgaca accccaggta ttgatgcacg
    10921 taacggcgaa tatgaagcgt tcggtgtacc cgcgacgatt cttgctaact tcctgcgcga
    10981 aaatggcgta gtgccggaaa aatgcgatct taactccatc ctcttcctgc tgactccggc
    11041 agaagatatg gccaaacttc agcaacttgt gccctgctgg tacgcttcga aaaactgctt
    11101 gagtccgacg cgccattagc agaagtgctg ccttccatct acaaacagca tgaagagcgc
    11161 tacgccggtt ataccctgcg tcagttgtgc caggaaatgc atgatttgta tgcccgccac
    11221 aacgtgaaac aactgcaaaa agagatgttc cgcaaagcgc acttcccaca cgtcagcatg
    11281 aatccgcaag aagccaacta cgcctattta cgcggtgaag tggagctggt tcgtctgccg
    11341 gatgccgaag gtcgtatcgc tgccgaaggg gcgcttcctt atcctccggg tgtgctgtgt
    11401 gttgttccgg gtgaaatctg gggtggtgct gttttgcgtt acttcagcgc tctggaagaa
    11461 gggatcaacc tgctgccagg ttttgcaccg gagctgcagg gtgtctatat cgaagaacat
    11521 gatggtcgta agcaagtttg gtgctatgtc atcaagcctc gtgatgcgca aagcaccctg
    11581 ttgaaagggg aaaaattatg agtcaggcta aatcgaacaa aatgggcgtc gttcagttaa
    11641 ccatactgac gatggtcaac atgatgggct ccggtatcat catgctgccg acaaagcttg
    11701 ccgaagttgg gacaatttca attatctcct ggctggtgac agccgtaggc tcaatggcac
    11761 tggcatgggc attcgcaaaa tgcggtatgt tcagccgtaa atcaggtggt atgggcggtt
    11821 atgccgagta tgctttcggt aaatccggta actttattgc gaactatacc tacggcgtct
    11881 cactgctgat tgctaacgtc gcgattgcta tttcggcggt tggttacggc accgaactgc
    11941 tcggcgcgag tttgtcgcca gtgcagattg gtcttgcgac catcggggtg ctgtggattt
    12001 gtaccgtggc taactttggt ggtgcgcgca ttaccgggca aatcagtagc attaccgtgt
    12061 gggggggcat tattccggtg gtaggcctgt gcattatcgg ctggttctgg tttagcccga
    12121 cgctttacgt tgattcctgg aatccgcatc atgcaccgtt cttcagtgca gtaggttctt
    12181 ccatcgctat gacgctgtgg gcttttcttg gtctggagtc tgcgtgtgcg aatactgatg
    12241 tagtggaaaa cccggaacgt aatgtgccaa tcgcggtact cggcggtacg ttaggtgcgg
    12301 cggtgattta tatcgtctcc accaacgtga ttgcagggat tgtgcctaat atggagctgg
    12361 caaattcaac ggcaccattt ggcctggcct tcgcacagat gtttacacca gaagtgggta
    12421 aagtcattat ggcgctgatg gtgatgtcct gctgcggttc gctgcttggc tggcagttca
    12481 ccattgccca ggtgtttaaa tcttcatctg atgaaggcta cttccctaaa attttctccc
    12541 gtgtaaccaa agtggatgca ccggtgcagg gaatgttgac cattgtgatt atccagagcg
    12601 ggttggcgct gatgacgatt agcccgtcgc taaacagtca gttcaacgtg ctggttaacc
    12661 tggccgtggt gaccaatatc attccgtata ttctgtcaat ggcggcatta gtcattattc
    12721 agaaggtcgc taatgtgccc ccctcaaaag cgaaagttgc aaactttgtt gcttttgttg
    12781 gcgcgatgta tagcttttat gcgctgtact catccgggga agaagccatg ctgtacggtt
    12841 ccatcgtgac cttccttggt tggacactgt atggtctggt atcaccacgc tttgaactga
    12901 aaaataaaca cggttaaaaa agcaaagtaa aaaagggcga tcttgcgacc gctctttttt
    12961 tattaaatgt gtttacgcgt ttttcagaac ttcgctaaca atctcaaccg cttctttctc
    13021 aatctgcttg cgatgttctt caccgaggaa gctttcgcag tagatcttat atgcgtcttc
    13081 cgtgcctgac ggacgcgcgg cgaaccagcc gttgtcagtc atcactttca gaccgccaat
    13141 agaagcaccg ttgcccggag cagcagtcag gcgcgcggtg atcgggtcac ctgccagggt
    13201 gctggcgctc accatttccg gagacagctt agacagcgcc gctttttgtg cggaagtcgc
    13261 agctgcctgc aaacggttgt agctcggcgc accaaagcgt tttgccagtt cgttgtagtg
    13321 ttcctgcggg ttcttaccgg tgacagcggt gatttccgcc gccagcagac acataatgat
    13381 gccgtctttg tcggtggacc acggcgtgcc gtcgaaacgc aggaaggaag cccctgcgct
    13441 ctcttcgccg ccaaagccga agctgccgtc gaacagacca tcaacaaacc atttgaaacc
    13501 taccggtact tctaccagct tacggcccaa gtcattgacc acacggtcga tcatcgcaga
    13561 agaaaccagc gttttaccga cagcaacatc tttgccccac tgcggacgat gctggaacag
    13621 gtagttgatc gccaccgcca ggtagtggtt cggattcatc aaacctgccg gagtgacgat
    13681 accgtgacgg tcataatccg ggtcgttagc aaacgccaga tcgaacttat cacgcagtgc
    13741 cagcagaccc gccatcgcac actcggagga gcagtccata cggatcgcac cgtctttatc
    13801 gaggtgcata aagcggaagg tttgatcgac ctgatcgtta acgatggtca ggttgaggtt
    13861 gtaatactcg ccaatgcgct tccagtattc gataccggaa ccgcccaacg gatcaacgcc
    13921 cagcgtcagg cccgctttct gaatcgccgc catatcaacg atatcggcca gcccttccac
    13981 gaacggctgc accagatcct gctctttcac atagccggat gccatcgctt cgtcgaggga
    14041 gatacgcttc acgcctttca ggccatcggc catcagtgcg ttggccctgt cttccaccac
    14101 tttggtgacg ttagtatcag ccgggccacc atttggcgga ttgtacttga taccaccatc
    14161 ttccggcggg ttatgggacg gtgtaatcac gataccgtct gccagcgggc cacctttttt
    14221 attgtgaacc aggatggcat tggaaacggc aggcgtcgga gtgaagccat tgttttcctg
    14281 cacaatgaca tcaacgccgt tcgctgccag cacttccagt acggaaatga acgcaggctc
    14341 ggacagggcg tgagtatctt tacccacata gcaagggcca gtgatgccgt ttttcgcacg
    14401 ttcttcagca attgcctgag cgatcgccag aatgtgcggc tcgttaaagc tgtggcgcgc
    14461 tgcactgcca cggtgaccgg aagtaccgaa tttcaccgcg tgctccgcat tccctgcttc
    14521 tggtttcagt acataatatt gcgccgtcag ttgggcgacg ttaatcaaat cactctgttg
    14581 tgcaggttgg cctgcacgat tgtggattgc cattgctttg tcctttgtct gcaacgtttt
    14641 agatagttcc gcaaaccttc tcaatcaatt ccgccgggaa ttgcatcgac tgcatgatgt
    14701 gttcgatcat gctgcattta cggccggtgt tggtgttggt gatcacccaa tacggcgtgc
    14761 ctggcacatg tttcggcttg gtctgattac catttttcag cagcgtttgt tcatctgccg
    14821 caaagtacac gcgtgtacga ccgtgcaacg attccgttgc ttcggcaaac gcctgggcgt
    14881 caagagaata tagtgtagac aacagcagca taaagcgatt gaccgctcgc ttttgctctg
    14941 cgtattcgtc cgaaagcaga agttcacgca ttgcgcgaac cttgtcttta atcgttttga
    15001 ccggcttcgc ttcgacgata gcaggtgacg caacgcgaac ctctttcgtc accggagcag
    15061 caggctgtga tgcggcggaa aatttcaaca tacgccgtaa aatgtcggat gcgctctcgc
    15121 cgatatgctt agtgtggctg gcaatatagc tgtagagttc atcatcaact tcaatcgttt
    15181 tcatcttaat ccagtgcagt gtcttagctg aatacaagtc gccaagagta tatgaataaa
    15241 tcccggcagc ggatagcgtc aagcctggct tgcagaaaaa ctcggaataa accttatttg
    15301 ttgcagccct gtggcacaat ggtcaacata ataccctaac ccgacaacgc gaaaaaaaga
    15361 actttgccat gaaattaaat atccgcgcgc aaactgcaca aaaccagcac aataattctc
    15421 ccatcgttct tgtccatggt ctgtttggta gcctcgacaa ccttggcgta ctggctcgcg
    15481 atctggtaaa cgatcacaat atcatccagg ttgatatgcg taaccacggt ctttcaccga
    15541 gagagccggt aatgaattac ccggcgatgg cgcaggatct tgttgatacc ctggatgcac
    15601 tgcagatcga caaagcaaca tttatcggtc actccatggg cggtaaagcg gtaatggcac
    15661 ttactgcact ggcccccgat cgcatcgata aactggtggc gatcgatatc gcaccggtcg
    15721 actatcacgt acgccgtcat gatgagattt tcgcagctat caacgcggtc agtgaatcgg
    15781 acgcacaaac tcgccagcaa gcagcagcaa taatgcgcca gcatcttaat gaagaagggg
    15841 tgattcagtt tctgttgaaa tcttttgttg acggggagtg gcgctttaac gtgccggtat
    15901 tgtgggatca gtatccgcat attgtgggtt gggagaaaat cccggcatgg gatcaccctg
    15961 ccctgtttat ccctggcggc aattctccgt atgttagcga gcagtaccgt gatgatttac
    16021 tggcacaatt tccacaggca cgggcgcatg tgattgcagg cgcgggtcac tgggtccatg
    16081 ctgaaaaacc ggatgcggta ttacgcgcta tccgtcgcta tctcaatgat taacgcaact
    16141 gattaaaaac ccagcgtccg cgtcgctttc cgcgcgtggg cgttggcgtg actcatcgcg
    16201 ctgatgtatt atggcgcgct atccatccgg gcagaggcct ggctgattgt ttcccccgaa
    16261 gtcaccaaga tcatggccaa agaacaaacg gaccgtacga cattagatct gttcgcgcac
    16321 gagcgtcgac cgggacgacc gaaaactaat ccgctttcgc gcgatgaaca gctgcgtatt
    16381 aataaacgca accagctaaa acgcgacaaa gtacgtggcc ttaagcgtgt cgaactgaag
    16441 ctgaacgcgg aagctgtcga ggcgctgaac gagctggcgg aatcgcgtaa tatgagccgt
    16501 agcgaactga tcgaagagat gttaatgcag caactggcgg cgctgcgtag tcagggcatc
    16561 gtttaaattt ccactttcat gtagcacagt gtgcagtcct gctcgtttgc tgattccgca
    16621 agactgcctg ttctgctatg attgccttta tccgtgggca attttccacc cccatttcaa
    16681 taagtttcaa gaggttattt cactcatggc tatcactggc atctttttcg gcagcgacac
    16741 cggtaatacc gaaaatatcg caaaaatgat tcaaaaacag cttggtaaag acgttgccga
    16801 tgtccatgac attgcaaaaa gcagcaaaga agatctggaa gcttacgaca ttctgctgct
    16861 gggcatccca acctggtatt acggcgaagc gcagtgtgac tgggatgact tcttcccgac
    16921 tctcgaagag attgatttca atggtaaact ggttgcgctg tttggttgtg gtgaccagga
    16981 agattacgcc gaatatttct gcgacgcatt gggcaccatc cgcgacatca ttgaaccgcg
    17041 cggtgcaacc atcgttggtc actggccaac tgcgggctat catttcgaag catcaaaagg
    17101 tctggcagat gacgaccact ttgtcggtct ggctatcgac gaagaccgtc agccggaact
    17161 gaccgctgaa cgtgtagaaa aatgggttaa acagatttct gaagagttgc atctcgacga
    17221 aattcttaat gcctgatgtg atgcggcgta gactcatgtc tacgccgtat taatagataa
    17281 tgccaatcaa aataattgct acaaatttgt aacttttgct gttgtacctg tacaatgtcc
    17341 cagtgttcaa gtggccttgc cgttgtaaat gtaagctgtg ccacgttttt attaacaata
    17401 tttgccaggg acttgtggtt ttcatttagg cgtggcaatt ctataatgat acgcattatc
    17461 tcaagagcaa attctgtcac ttcttctaat gaagtgaacc gcttagtaac aggacagatt
    17521 ccgcatgact gataacaata ccgccctaaa gaaagctggc ctgaaagtaa cgcttcctcg
    17581 tttaaaaatc ctggaagttc ttcaggagcc ggacaaccat cacgtcagtg cggaagattt
    17641 atacaaacgt ctgatcgata tgggtgaaga aattggtctg gctacggtat atcgcgtact
    17701 gaaccagttt gacgacgctg gtatcgtcac ccgccacaat tttgaaggcg gtaaatccgt
    17761 atttgaactg acacagcaac atcaccacga tcacctgatt tgcctcgact gcggcaaggt
    17821 tatcgaattt agtgatgatt ccatcgaagc gcgtcagcgt gaaattgccg caaaacatgg
    17881 cattcgcctg actaaccaca gtctctatct ttacggtcac tgtgccgaag gcgattgccg
    17941 cgaagatgag cacgcgcacg aaggcaaata agccagcctg aacgagaaaa gccaacctgc
    18001 gggttggctt ttttatacaa gggaaataag aaaagcgaga gttacagctc tcacttattt
    18061 gttattactg cgtatttcct gccagacctt atcacaatcg gctttcaccg cctgatcatt
    18121 tccggtttgc gttgcacgca gacactgctg ataatccagt acgcgtacac tttcctggtc
    18181 ggcaaactgc tgatgttgtt tctctttctt cagcacgttt aacacgctct ggcaggcttc
    18241 aattttttct ggcgaacctt gcgcggtgtt gatacaggcg ctgtatgcct ctttcagacg
    18301 gctgtcttcc tgtggtgcgg ttgattgcgc acaagctacc agccccgatg ccatcatggc
    18361 gatgagaatc agttttttca ttgaatgcgg caggcacaaa acctgccgcg tcgggcatca
    18421 gaagatggtg aatggtgcga ttaccataaa tttcacgtca cgctcatcct ggaagatgtt
    18481 accgtaaccg ccgccccagc ttgggatgtc ggagtggttg tcgtattcgg tgaagtgcag
    18541 tttgaacatc gtgcctttgg cgcgaccgtc ctgaatggtg tagaccgcat ccaggctgta
    18601 ggcagactct tcaatagtcc ggtttttgtc gtagtacgca tccggattgc tctgccaggt
    18661 cgcaggttta gcatcccatg cgtaaacgta ggaagcgccg atggcgaagc ctggaagatt
    18721 ccagtttttc aggtcataca tcgcactgaa gaagaccgct ttttcgccgt tagcgttgaa
    18781 gtcggaacgg ttatcccacc agatatccag gcggccgttg gaggaagcgt aggttggggt
    18841 catacgttgc aggaagtatc cctgctgact gtcagcctta atccaggtgc cttcgaggcg
    18901 caaatccact acgtcagccg cccggtaacc aaaggtcaac gcttgcagcc aggcggtgcc
    18961 gtcataaaga tcgttgacgc tgcgatcgtc aactttatcg cgggtaccat agaactggta
    19021 gctggtggtt aacgggctac cggcgatatc aaatttgtag ctggctttgg caaaatattg
    19081 atcgatatac ccttccgcct gaccaaatgc cgcttccagt acgaagttat ttttgaagtc
    19141 gtatttcgca ccgatggagt gcagataatc aactttggtg gttttatcgt tctgataaaa
    19201 ctcatccatt tccagatgcc acggcgcttt gtattcgttg gtccacatgt aggagaaact
    19261 caaggcacca gcatcgccgt aatcaaaatt cgccccggct tccgccccct gataagtacc
    19321 cggcataaag ctccagtgcg gcgctaacag cgtttgaccg gttggctgaa tgtaacctgc
    19381 cctcgcccaa accggaccgt atttaaattt ggccgcagct ttatacaggc ttataccgct
    19441 tttgtcgccg gaccagtctt cgtcataggc tttattactt tttgaaaacg cgatttcgtt
    19501 cgggtgggag ctgtcgccgt tttccgccat ttcaatcgcc gtaaacgcag caatatcaag
    19561 gccgaacata tcagcagcat aaccggactg aaaatcgagg ttggcattcc aggtggagtg
    19621 agaaaggttg gttttgtatt tgtcgccgtc ggtaacatct ttacggtcgc gttcacgctg
    19681 ccagtaatag ataccgccgg ttaaggttga atcgtcgatg aatccttcgg cgcgagcctc
    19741 cggcattgcc ataaagcccg acattgctgt aacaccggca atagccagcg ccagcgtact
    19801 acgtttgcca ctaaacgtac gcatgggtta atcctctttg acgtataaat tgctgcacca
    19861 aaggtgaaca gcgaaaaaaa ataaaaatta aaaaagtagc gttgagaaaa gtctcgctga
    19921 ctacaggaac agactatttt tcgcgacgcg aattatcaat ctctttctgt aaccagaatg
    19981 taagaacatg agcaaccgca catattttga tgatttttgt tacaaggctt aagtttgcgg
    20041 ttgaaaggct tgcagaagaa taaaaacgtg aaattccagc aagatgaaag cggttacatt
    20101 ttatttaatt atgttttgtg attttaaatt tattttatgg ataaattgcg aagcggaatt
    20161 aattcgcgaa gaaaagcaga aaaaacgccg cagagcgcgg cgtttgggat tgtttgctat
    20221 ttcttgccgg atgcggcgtg aacgccttat ccggcctaca aaagcacgca aattcaatat
    20281 attgcagaga tcatgtaggc ctgataagcg aagcgcatca ggcaatttag cgtttgaaat
    20341 ttattgtcta tttattgccg gatgcgacgt aaatgcctca cccggcttgg tagaacttaa
    20401 aattactcgc ctactttcgc ccaggtatca cgcagcccaa cggtgcggtt aaataccggt
    20461 ttttcggccg tggaatggcg gctgtcgagg cagaagtaac cttcacgctc aaactggaat
    20521 gctttacccg caaccgcatc tttcagcgac ggttcagcaa agccctgttt gatcaccagc
    20581 gattccgggt taatcaccga caggaaatca tccgcagcac ctgggttagg cacgctgaac
    20641 agacgatcat acaaacggat ttcaaccggc agcgcatgtg ccgcgctcac ccagtgaata
    20701 acacctttga ctttacgacc atctgccgga tctttgctta aggtgtcggc gtcataagta
    20761 cagaagatgg tggtgatatt accttcggca tctttctcca cgcgttcagc cttaatgacg
    20821 taagcattac gcagacgcac ttctttaccc agcaccagac gtttgtactg cttgttagct
    20881 tcttcgcgga aatcggcgcg atcgatccaa atttcaccgc taaacggcac ctgacggctc
    20941 cccatttccg gtttgttcgg atggttcggc atggtgacca tttcgccttc gccctgatag
    21001 ttttcgataa ccagtttcac cggatcgata accgccattg cgcgcggcgc attttcgttg
    21061 agatcttcac ggatgcagga ttccagcgac gccatctcaa tggtgttgtc ctgcttggtc
    21121 acgccgatgc gtttgcagaa ctcacgaata gaagccgcag tgtaaccacg acgacgcaga
    21181 ccggaaatgg tcggcatacg cgggtcatcc cagccttcaa cgtgcttgtc ggtcaccagc
    21241 aggttcagct tacgcttgga catcacagtg tattccagat tcaggcgcga gaattcgtac
    21301 tggcgcggat gaacaggaat ggtgatgtta tccagtaccc agtcgtacag acgacggttg
    21361 tcctggaact caagcgtaca cagagagtgc gtaatacctt ccagcgcatc gctgatgcaa
    21421 tgggtgaagt cgtacatcgg gtagatgcac cacttgttgc cagtctggtg gtgttcagca
    21481 aatttaatgc ggtacagcac cggatcgcgc attacgataa atggcgacgc catgtcgatt
    21541 ttcgcacgca ggcaggcttt accttcttca aaaccaccgg cacgcatttt ttcgaacagc
    21601 gccaagttct cttcaacgct gcggtcgcgg tacgggctgt ttttacccgg ctgcgtcagg
    21661 gtaccgcggt attcgcggat ctgttccggt gtcagttcat caacgtacgc caggccttta
    21721 ttgatcagtt cgatcgcata ggcgtggagc tgatcaaaat aatcggagga gtaacggacg
    21781 ttaccagacc agtgaaaacc taaccactct acgtcgtttt tgatcgaatc gacatactcg
    21841 atatcttctt ttaccgggtt agtgtcgtcg aaacgcaggt tgcactggcc tttatagtcc
    21901 tgggcgatcc cgaagttcag gcagatagat ttcgcatggc caatatgcag atagccattc
    21961 ggctccggcg ggaaacgggt gtgtactgtg atgtgcttac cactggccag atcttcatcg
    22021 atgatctgac ggataaagtt agtcgggcgg gcttctgcct cactcatcgt ggattcctca
    22081 aagcgtaaac aacgtataac ggcgtatgat cttataagcg ggacaggctg acaactgtta
    22141 gttttttaaa attgataatc taacaaggat tatggggatg attgatgaaa aaaaaagcgg
    22201 cagagaagat ctccaccgct tgtcattgtt ggatgcgacg ctgccgtgtc gcatcaggca
    22261 taaagcagat tactttttga tttcatacag cggtgtttga cccgccacaa catggccctg
    22321 agctttaatg agcaagccac tgaaatcgtc gatattgctg caaaccaccg ggctaatcat
    22381 cgagcgggcg ttagcgttca ggtaatccag atccatttcc agaatcggtt gccctgcgct
    22441 tacctgcgca ccctcttcca ccagacgttt aaagccttta ccttccagcg ctacggtgtc
    22501 gatacccata tggacgacga tctccgcgcc tttttcggtt tccaggcaga acgcgtggtt
    22561 ggtgttgaag attttcacga ttgtccctgc ggctggtgat acgacgattt tatctgtcgg
    22621 tttcaccgcc acaccgtcac ccaccgcttt gctggcgaat gcttcgtcag gaacctgatc
    22681 cagtgccacg acatcaccgg taatcggcga taccagctcc gcgatagata ccgcgtttgg
    22741 tacagcctgc ggttttgcta caggcgcggc agttgccgga gttgcttcag ctgacgcagc
    22801 ggctaccgga ccacgggcaa cgactttctt catcgcatcg ccgatggatt ctgctttcgc
    22861 gccaacaatc acctgaatag tctgtttgtt cagtttcact acaccagaag cacccagacg
    22921 tttacacatc gtatcgttga cgcgggcaga gtcagccacg gtcaggcgca gacgggtaat
    22981 acaggcatca atcgctttca ggttgtcagt accgccaacc gcagcaatat agttggttgc
    23041 cagttgagtc agaccttctt cagtattgct gttggcttct tcagtaacga tctcgtcttc
    23101 tttatcttca cgacccggcg ttttcaggtt gaacatgcgg ataaccaaac tgaacaccac
    23161 gaagtagata gcgaagaaga taacgcccat caccagcagc atccagacgt tctggctggc
    23221 ggcaggcagg ttatacatca acgcgtagtc gatagcaccc gcagagaaag agaagccggc
    23281 atggataccc agcagcgttg ccacaaacag gctgataccg gtcagcagtg cgtgcaggag
    23341 gtacagcagc ggcgcaagga acatgaacag gaattccagc ggctcagtca caccggtcag
    23401 gaacgcagta acagcaacag aaagcagcat cccgccaacc atcggacgac gctctttcgg
    23461 tgctgcgaag tacatcgcca gcgccgcacc cggcagaccg aacatcatga tcgggaagaa
    23521 gccggacatg aacatccccg cggtgccgtc accagcgtag aaacggttga tgtcgccgtg
    23581 gaaaaccgta cccgccgcgt tggtgaattc accaatctgg aaccaggcga tggtgttcag
    23641 cacctgatgc agaccggttg ggatcagcag acggttgatg aaaccaaaga taccggaacc
    23701 cagcgcgccc gcagaaacga tccactcgcc gcctgcatgg atagcgtgct gtaccggcgg
    23761 ccagacgtaa ccaaaaatgg ccgccagcac cagacagaag aagccggtgg cgatcggcac
    23821 aaagcgtttg ccgccgaaga agctcaggaa gtccggcagt ttaatatcgg accaacggtt
    23881 ataggctgcg ccaccaacca gaccggtaat gatacccgcc agtacaccca tgttaatttc
    23941 tgggttgatg gtcaccatcg ctttggttaa cacaaagtaa cctaccgcac ccgccagcgc
    24001 cgccgcacct gcgctgtctt tcgaccagct ggatgccaca ccgatggcga agattaatgc
    24061 gaggttatca aaaattgcac cgcccgcctg ggcaataaac gcaacgttaa gtaaatctgg
    24121 ctgaccgaat cgcagcaaca gcgccgccac cggcagcacc gcgataggga gctgtaacgc
    24181 cctaccgagt cgctggaaaa aacctaaaat attcatctta ttccccctac gagaacccta
    24241 tttggctcgt ttaaagccgt atttttattt tgctgcaaat tttactgccg atgttctgta
    24301 atcagattgt tagatcatct gctacagagt gtgtgaaaat ttaattcgca tcgcaaatta
    24361 aacgcgtgtc ttttgtgagt tttgtcacca aatatcgtta tcatcactcc cttttactgg
    24421 ctaaaccaga aaacttattt tatcattcaa aaaatcaagt cggattgacg cctgtctgcg
    24481 caaatccagg ttacgcttaa agatgtctaa tccgccaacg gcttacattt tacttattga
    24541 ggtgaataat gagactgatc cccctgacta ccgctgaaca ggtcggcaaa tgggctgctc
    24601 gccatatcgt caatcgtatc aatgcgttca aaccaactgc cgatcgtccg tttgtactgg
    24661 gcctgccgac tggcggcacg ccgatgacca cctataaagc gttagtcgaa atgcataaag
    24721 caggccaggt cagctttaag cacgttgtca ccttcaacat ggacgaatat gtcggtctgc
    24781 cgaaagagca tccggaaagc tactacagct ttatgcaccg taatttcttc gatcacgttg
    24841 atattccagc agaaaacatc aaccttctca acggcaatgc cccggatatc gatgccgagt
    24901 gccgccagta tgaagaaaaa atccgttctt acggaaaaat tcatctgttt atgggcggtg
    24961 taggtaacga cggtcatatt gcatttaacg aaccggcgtc ttctctggct tctcgtactc
    25021 gtatcaaaac cctgactcat gacactcgcg tcgcgaactc tcgtttcttt gataacgatg
    25081 tcaatcaggt gccaaaatat gccctgactg tcggtgttgg tacactgctg gatgccgaag
    25141 aagtgatgat tatggtgctg ggtagccaga aagcactggc gctgcaggcc gccgttgaag
    25201 gttgcgtgaa ccatatgtgg accatcagct gtctgcaact gcatccgaaa gcgatcatgg
    25261 tgtgcgatga accttccacc atggagctga aagttaagac tttaagatat ttcaatgaat
    25321 tagaagcaga aaatatcaaa ggtctgtaat tgttatccct gccctggttc cttgctcagg
    25381 gcaatatttt ttaaaatcgg gggtcagaat gtatgcatta acccagggcc ggatctttac
    25441 cggccacgaa tttcttgatg accacgcggt tgttatcgct gatggcctga ttaaaagcgt
    25501 ctgtccggta gcggaactgc cgccagagat cgaacaacgt tcactgaacg gggccattct
    25561 ctcccccggt tttatcgatg tgcagttaaa cggctgcggc ggcgtacagt ttaacgacac
    25621 cgctgaagcg gtcagcgtgg aaacgctgga aatcatgcag aaagccaatg agaaatcagg
    25681 ctgtactaac tatctgccga cgcttatcac caccagcgat gagctgatga aacagggcgt
    25741 gcgcgttatg cgtgagtacc tggcaaaaca tccgaatcag gcgttaggtc tgcatctgga
    25801 aggtccgtgg ctgaatctgg taaaaaaagg cacccataat ccgaattttg tgcgtaagcc
    25861 tgatgccgcg ctggtcgatt tcctgtgtga aaacgccgac gtcattacca aagtgaccct
    25921 ggcaccggaa atggttcctg cggaagtcat cagcaaactg gcaaatgccg ggattgtggt
    25981 ttctgccggt cactccaacg cgacgttgaa agaagcaaaa gccggtttcc gcgcggggat
    26041 tacctttgcc acccatctgt acaacgcgat gccgtatatt accggtcgtg aaccgggcct
    26101 ggcgggcgcg atcctcgacg aagctgacat ttattgcggt attatcgctg atggcctgca
    26161 tgttgattac gccaacattc gtaacgctaa acgccttaaa ggcgacaaac tgtgtctggt
    26221 taccgacgcc accgcgccag caggtgccaa cattgaacag ttcatttttg cgggtaaaac
    26281 aatatactac cgtaacggac tttgtgtgga tgagaacggt acgttaagcg gttcatcctt
    26341 aaccatgatt gaaggcgtgc gtaatctggt cgaacattgc ggtatcgcac tggatgaagt
    26401 gctgcgtatg gcgacgctct atccggcgcg tgcgattgac gttgaaaaac gtctcggcac
    26461 gctcgccgca ggtaaagtag ccaacctgac cgcattcaca cctgatttta aaatcaccaa
    26521 gaccatcgtt aacggtaacg aggtcgtaac tcaataagag aaagtatgac accaggcgga
    26581 caagctcaga taggtaatgt tgatctcgta aaacagctta acagcgcggc ggtttatcgc
    26641 ctgattgacc agtacgggcc aatctcgcgg attcagattg ccgagcaaag ccagcttgcc
    26701 cccgccagcg taaccaaaat tacgcgtcag cttatcgaac gcgggctgat caaagaagtt
    26761 gatcagcagg cctccaccgg gggccgccgc gctatctcca tcgtcaccga aacccgcaat
    26821 ttccacgcaa tcggcgtacg gcttggtcgt catgacgcca ccatcactct gtttgatctc
    26881 agcagcaaag tgctggcaga agaacattac ccgctgccgg aacgtaccca gcagacgctg
    26941 gaacatgccc tgctgaatgc cattgctcag tttattgata gctaccagcg caagctgcgc
    27001 gagctaatcg ccatttcggt gatcctgcca gggcttgttg acccggacag cggcaaaatt
    27061 cattacatgc cgcatattca ggtagaaaac tgggggctgg tagaagcact ggaagaacgt
    27121 tttaaagtga cctgtttcgt tggtcacgat atccgtagtc tggcgctggc ggagcactac
    27181 ttcggtgcaa gtcaggattg cgaagactcc attctggtgc gtgtccatcg cggaaccggg
    27241 gccgggatta tctctaacgg gcgcattttt attggccgca acggtaacgt cggtgaaatt
    27301 ggccatattc aggtcgaacc gctgggtgaa cgctgccact gcggcaactt tggctgcctg
    27361 gaaactatcg ccgccaacgc tgccattgaa caacgggtgt tgaatctgtt aaagcagggc
    27421 taccagagcc gtgtgccact ggacgactgc accatcaaaa ctatctgcaa agccgcgaac
    27481 aaaggcgata gcctggcctc ggaagtgatt gagtatgtcg gtcgtcatct gggtaaaacc
    27541 atcgccattg ctatcaactt atttaatccg caaaaaattg ttattgctgg tgaaatcacc
    27601 gaagccgata aagtgctgct ccctgctatt gaaagctgca ttaataccca agcgctgaag
    27661 gcgtttcgta ctaatctgcc ggtggtacgt tctgagctgg atcaccgctc ggcaatcggc
    27721 gcttttgcgc tggtaaaacg cgccatgctc aacggtattt tgctccagca tttgctggaa
    27781 aattaatgtg cttttatagt ggcgcttatt gttgtcaata ttctgggtag tccatgacca
    27841 ttaaaaatgt aatttgcgat atcgacggcg tgctgatgca cgataacgtc gccgtaccgg
    27901 gtgcagcgga atttttgcac gggattatgg ataaaggcct gccgctggtg ttgctgacca
    27961 actatccttc gcagactggg caagatctgg cgaaccgctt tgccactgcc ggtgtcgatg
    28021 tgccggatag cgtgttttat acctctgcga tggcgaccgc cgatttcctg cgtcgccagg
    28081 aaggcaagaa agcgtatgtg gtgggcgaag gcgcactgat tcatgaactg tacaaagccg
    28141 gtttcactat taccgatgtg aaccctgatt tcgtgattgt tggcgaaaca cgttcctaca
    28201 actgggacat gatgcataaa gcagcctatt tcgtcgctaa cggtgcacgc tttatcgcca
    28261 ccaacccgga cacccacggg cgcggttttt atcccgcttg tggcgcgttg tgtgcaggga
    28321 ttgagaaaat ctccgggcgc aaaccgttct atgttggtaa gcccagcccg tggatcatcc
    28381 gcgcggcatt aaacaaaatg caggcgcatt cggaagaaac agtgattgtc ggcgataacc
    28441 tgcgtaccga tattctggcg ggcttccagg ccggtctgga gacgattctg gtgctttctg
    28501 gtgtttcatc gctcgacgat atcgacagta tgccgttccg cccaagctgg atttacccgt
    28561 cagtcgctga aatcgacgtt atctgaatac cacccacgtc tgcggacgtg gttggctctg
    28621 atgacaaacg ccaaactgcc tgatgcgcta cgcttatcag gcctacgcag ctcctgcaat
    28681 atattgaatt ttcatgcttt tgtaggccgg ataaggcgtt cacgccgcat ccggcatgaa
    28741 caaatcgcac tttatcagca atttggcccc cgtaaaatat aaccattcaa taaaacccgc
    28801 taaaaattat cgattcatca ataaatacat cctcacacca gacccttttt caccaatcga
    28861 caatcaccat tacgtttttt atttttgcac tgccaaatag cttgcgcaca gtgctgcttt
    28921 gcggcatttt tttaaacaag caaacacaac aagcaacaaa taccaggtta acggagaagg
    28981 ttatgtgttc aatttttggc gtattcgata tcaaaacaga cgcagttgag ctgcgtaaga
    29041 aagcactcga gctgtcacgc ctgatgcgtc atcgtggccc ggactggtcc ggtatttatg
    29101 ccagcgataa cgccattctc gctcacgaac gtctgtcaat tgttgacgtt aacgcggggg
    29161 cgcaacctct ctacaaccaa caaaaaaacc acgtactggc ggtaaacggt gaaatctaca
    29221 accaccaggc attgcgcgct gaatatggcg atcgttatca gttccagaca gggtctgact
    29281 gtgaagtgat cctcgcgctg tatcaggaaa aagggccgga atttctcgac gacttgcagg
    29341 gcatgtttgc ctttgccctg tacgacagcg aaaaagatgc ctacctgatt ggtcgcgacc
    29401 atctggggat catcccactg tatatggggt atgacgaaca cggtcagctg tatgtggcct
    29461 cagaaatgaa atcgctggtg ccagtttgcc gcacgattaa agagttcccg gcggggagct
    29521 atttgtggag tcaggacggc gaaatccgtt cttattatca tcgcgactgg ttcgactttg
    29581 atgcagtgaa agataacgtg accgacaaaa acgagctgcg tcaggcactg gaagattccg
    29641 ttaaaagcca tctgatgtct gatgtgcctt acggtgtgct actttctggt ggtctggatt
    29701 cctcaattat ttccgctatc accaagaaat acgcagcccg tcgcgtggaa gatcaggaac
    29761 gctctgaagc ctggtggccg cagttacact cctttgctgt aggtctgccg ggttcaccgg
    29821 atcttaaagc agcccaggaa gtggcaaacc atctgggcac ggtgcatcac gaaattcact
    29881 tcactgtaca ggaaggtctg gatgccatcc gcgacgtgat ttaccacatc gaaacttatg
    29941 atgtgaccac tattcgcgct tcaacaccga tgtatttaat gtcgcgtaag atcaaggcga
    30001 tgggcattaa aatggtgctg tccggtgaag gttctgatga agtgtttggc ggttatcttt
    30061 acttccataa agcgcccaac gctaaagaac tgcatgaaga gacggtgcgt aaactgctgg
    30121 ccttgcatat gtatgactgc gcgcgcgcca acaaagcgat gtcagcctgg ggcgtggaag
    30181 cacgcgttcc gttcctcgac aaaaaattcc ttgatgtggc gatgcgcatt aacccgcagg
    30241 ataaaatgtg cggtaacggc aaaatggaaa aacacatcct gcgtgaatgt tttgagtcat
    30301 acctgcccgc aagcgtggcc tggcggcaga aagagcagtt ctccgatggc gtcggttaca
    30361 gttggatcga caccctgaaa gaagtggcgg cgcagcaggt ttctgatcag caactggaaa
    30421 ctgcccgctt ccgcttcccg tacaacacgc cgacctcaaa agaagcgtat ctgtaccgcg
    30481 agatctttga agaactgttc ccacttccga gtgccgctga gtgcgtgccg ggcggtcctt
    30541 ccgtcgcgtg ttcttcagct aaagcgatcg aatgggatga agcgttcaaa aaaatggacg
    30601 atccgtctgg tcgtgcggtt ggcgttcacc agtcggcgta taagtaagaa atgcggtact
    30661 gccccggaga aatccggggc tgttttagtt attgcgctgc ttgttgattc tgatatgcag
    30721 taaggaaatg ttccgggttt gccacatcac tgtttgacaa ctcatcactg taggccagta
    30781 tttcatagat taatttccca ccactattgt tgaattccga atttcatcct gtacgacaaa
    30841 agaatggctg tagggtgggg aaaaacacaa agtaaacaat aattgacgaa tatagcgcca
    30901 cgctgttcgc aacctaacca aacagtcact ttcgagcaat tttccttgaa aaagaggttg
    30961 acgctgcaag gctctatacg cataatgcgc cccgcaacgc cgataaggta tcgcgaaaaa
    31021 aagatggcta cgtagctcag ttggttagag cacatcactc ataatgatgg ggtcacaggt
    31081 tcgaatcccg tcgtagccac catctttttt gcgggagtgg cgaaattggt agacgcacca
    31141 gatttaggtt ctggcgccgc aaggtgtgcg agttcaagtc tcgcctcccg caccattcac
    31201 cagaaagcgt tgtacggatg gggtatcgcc aagcggtaag gcaccggttt ttgataccgg
    31261 cattccctgg ttcgaatcca ggtaccccag ccatcttctt cgagtaagcg gttcaccgcc
    31321 cggttattgg ggtatcgcca agcggtaagg caccggtttt tgataccggc attccctggt
    31381 tcgaatccag gtactccagc catcgaagaa ataatctggc tacgtagctc agttggttag
    31441 agcacatcac tcataatgat ggggtcacag gttcgaatcc cgtcgtagcc accaaattct
    31501 gaatgtgtcg aatacgttcg gcaaattcaa aaaccaattt gttggggtat cgccaagcgg
    31561 taaggcaccg gattctgatt ccggcattcc gaggttcgaa tcctcgtacc ccagccaatt
    31621 tattcaagac gcttacatcg taagtgcacc cagttggggt atcgccaagc ggtaaggcac
    31681 cggattctga ttccggcatt ccgaggttcg aatcctcgta ccccagccac attaaaaaag
    31741 ctcgcttcgg cgagcttttt gcttttctgc gttcattcaa tgtcgaatgc gatgttgaca
    31801 cgtcttatcc ttcaatgtcg gatgcgacgc tgccgcgtct tatccgacct acggctggca
    31861 cgcatccggc aatgttgtaa ggctacaacc ctaacgcata tttcagcgcc tgacgtttca
    31921 acacgccagc acgctctgcc gccattaacc cgagattacg cacaaaacgc agcggcggca
    31981 gattattact gaatccggca taaaacagat ccataccact ttgcataatg aagttatccg
    32041 ccatgcgccg catctggtaa cgcttgagga caggataact ggcccacgct tcaccgtagc
    32101 tgcgggcatt caccagaaca tcaatcaggg catcgacatc acgataacca agattcaccc
    32161 cctgccccgc cagcggatgg atggtatgcg cggcatcgcc caccagcgca agccccggct
    32221 gcacatactg caacgcatgt cggcgcgtca gcggaaacgc accagcggca agcggtgtaa
    32281 cgtaacccag acgcgacggg aaatgcttcg cgatttccgc ctggagttgc gccatattca
    32341 tattctgcaa ctggcgaata cgcgccggag agtcatacca caccagcgat gcccagttat
    32401 caaacaacgg cagaaacgca cgcggtccgt ccggggtaaa ttgctgccag gtgctgtcgc
    32461 cgggatcgtt ctcacactgt acgctaatca acatgcacga ctgcgcatac tgccaggcat
    32521 gaacgccaat tcccgccatc tgccgcacct gagaatttgc gccgtcggca ccaatcacca
    32581 gtttcgcgcg gatcgtttca ccgcccttca gttccagctc ctgaagatca ttatctcgat
    32641 gtaatgcaat cagcgaggtt ggcacacgta acgttacttt cggatgcgct tccagcgcct
    32701 gccacaacgc ctgttgcagg actgtgtttt ccaccatgta gccaagcaac ggcaacttta
    32761 attcggctgc gtcaaatacc acatgtgccg tttcccactc ccaggtttcc agtctgcggt
    32821 aaggatggca acgcatagcc tgtactgcat cccagacccc taacccttta agcaatgata
    32881 ccgaagccgc gctgatcgcc gagatccgca cgtccggttg gctgtcagcg acaaacggtg
    32941 caggttctgc gtgctcgatc accgttaccg aaaatccgtg ctgtgccagc cccagcgcca
    33001 gtgcgccgcc gaccattcct ccgccgacaa tggcaatttc cgttggttga tttgtcatgg
    33061 tcaatcatcc tgttagcaat atgctttaag tttacaggat ttttaccccc aagggctgat
    33121 aacgctcata ctggtcacaa cggcagcaaa gcattacact atgcgccctg cattcctggc
    33181 tactatttcg caagagcaag tcgatgacca aaaaactcca tattaaaacc tggggctgtc
    33241 agatgaacga gtacgattca tcgaagatgg ccgatctgct ggatgccacc cacggctatc
    33301 aactgaccga cgtggcggaa gaagcggatg tgctactgct gaacacctgc tcaatccgcg
    33361 agaaggctca ggaaaaagtc ttccatcagt tgggtcgctg gaaactgtta aaagagaaga
    33421 atccagacct gattatcggc gtcggtggct gcgtggcatc gcaagaaggc gagcacattc
    33481 gccagcgcgc ccactatgtc gatattattt ttgggccgca aacgctgcac cgtctgccgg
    33541 agatgatcaa ctccgtgcgc ggcgaccgca gcccggttgt agatatcagc ttcccggaaa
    33601 tcgagaagtt tgaccgtctg ccggaaccgc gcgccgaagg gccgaccgcg tttgtctcca
    33661 tcatggaagg ctgcaataaa tattgcacct actgcgtggt gccttacacc cgtggtgaag
    33721 aggtaagccg tccgtctgac gatattctgt ttgagattgc ccagcttgcg gctcagggcg
    33781 tgcgtgaagt caacctgctc ggtcagaatg tgaatgcctg gcgtggtgag aactacgacg
    33841 gcaccaccgg atcgtttgcc gatctgttgc gtctggttgc tgcgatcgac gggatcgatc
    33901 gtattcgctt taccaccagc catccgatcg aattcaccga cgatatcatc gaagtgtacc
    33961 gcgacacgcc tgagctggtg agcttcctgc atctgccggt acagagcggt tccgatcgca
    34021 ttctgaacct gatgggacgt acccatacgg cgctggagta caaagcgatc atccgtaaac
    34081 tgcgtgcggc gcgtccggat attcagatca gttctgactt cattgttggc ttccctggcg
    34141 aaaccaccga agacttcgag aaaacgatga agctgattgc cgacgtcaat ttcgacatga
    34201 gctacagctt tatcttctct gcacgtccag gaacgcctgc cgccgatatg gttgatgatg
    34261 ttccggaaga agagaagaag cagcgtctgt atattctgca agaacgtatc aatcagcaag
    34321 cgatggcatg gagccgtcgg atgctcggca ccacccagcg tattctggta gaagggactt
    34381 cgcgtaagag catcatggag ctttccgggc gtaccgaaaa taaccgcgtg gtcaacttcg
    34441 agggcacacc ggatatgatc ggtaaattcg tcgatgtaga aattaccgac gtctacccga
    34501 actctctgcg cggtaaagtg gtgcgaactg aagatgaaat gggtctgcgc atggcagaaa
    34561 caccggagtc ggtgattgcc cgcacccgca aagaaaacga ccttggcgtg ggttattatc
    34621 agccgtaatt ctcaggcctg ccgttccggt gggccttgtt ttttctctct ttatccccat
    34681 ctttatcgca atgcttgcgc ctttttcctg tggcgtgaat aatttcctta taggccggtg
    34741 aatgttcagc ttccggcggc atacagataa gaggaacggt ttgaacatag acactcgcga
    34801 aatcaccctg gagccagcag acaatgcgcg tctgttgagc ctgtgcggcc cgtttgatga
    34861 caacatcaag cagctcgaac gccgtctcgg catcgagatc aatcgccgcg ataaccactt
    34921 taaactgacc ggccgtccga tttgcgtcac cgctgcggca gacattctgc gtagcctgta
    34981 tgtcgatact gccccgatgc gcggtcagat tcaggatatc gaaccggaac agatccacct
    35041 tgcgattaaa gaagcgcggg tactggagca aagcgcggag agcgtgccgg agtacggcaa
    35101 agcggtcaat atcaaaacta aacgcggcgt aattaagccg cgcacgccaa accaggcgca
    35161 gtacatcgcc aatattctcg accatgacat taccttcggc gttggcccgg cgggtacggg
    35221 taaaacctac ctggcagtgg ctgcggcagt tgatgccctg gagcgtcagg aaattcgccg
    35281 tattctgctg actcgtccgg cggtcgaagc cggtgagaaa ctgggcttcc tgcctggcga
    35341 tttaagccag aaagtagacc cgtatctgcg cccactgtac gacgcgctgt ttgaaatgct
    35401 gggctttgag aaagtcgaga aactgattga gcgcaacgtt attgaagtcg cgccgctggc
    35461 ctatatgcgt ggtcgtacgc tgaacgacgc gtttatcatt ctcgatgaga gccagaacac
    35521 taccatcgaa cagatgaaga tgttcctgac ccgtatcggt tttaactcaa aagcggttat
    35581 caccggcgac gtcacacaga tcgacctgcc gcgtaatact aaatcaggct tacgtcatgc
    35641 cattgaagtg ctggccgatg tcgaagagat cagctttaac ttcttccaca gcgaagacgt
    35701 ggttcgtcac ccggtggtgg cgcgtatcgt taacgcttat gaagcctggg aagaagcaga
    35761 acaaaaacgt aaagcggcgc tggcagcaga acgcaagcgc gaagaacagg aacaaaaatg
    35821 agtcaggtga tcctcgattt acaactggca tgtgaagata attccgggtt accggaagag
    35881 agccagtttc aaacatggct gaatgcggtg atcccgcagt ttcaggaaga gtcggaagtg
    35941 actattcgcg tggtcgatac cgccgaaagc cacagtctga atctgaccta tcgcggtaag
    36001 gataagccga ccaacgtgct ctccttcccg tttgaagtgc cgcctggcat ggagatgtcg
    36061 ctactgggcg atctggttat ctgccgtcag gtggttgaga aggaagctca ggagcaaggc
    36121 aaaccactgg aggcgcattg ggcgcatatg gtggtgcacg gcagtctgca tttgttaggt
    36181 tacgatcaca ttgaagatga cgaagcagaa gaaatggaag ccctcgaaac agagattatg
    36241 cttgctctgg gctatgagga tccgtacatt gccgagaaag aataatttgc caggcatttg
    36301 actggcagac gtccccgccg ttggtgatta acgacggcgt tttgattaac taacatgaga
    36361 acccatacga cgccatgagc gacgacaatt cacacagtag tgacacgata agcaacaaga
    36421 agggattttt ctccctgtta ctcagccaac ttttccacgg tgaaccgaaa aaccgtgacg
    36481 aactgctggc gctgatccgt gattccgggc agaacgacct tatcgacgaa gatacgcgcg
    36541 atatgctcga aggggtgatg gacatcgcag accaacgcgt tcgcgacatc atgatccccc
    36601 gctcccagat gattaccctg aaacgcaacc agacgctgga cgaatgcctt gatgtcatca
    36661 tcgagtccgc ccactcacgt ttcccggtga ttagcgaaga caaagatcac attgaaggga
    36721 ttctgatggc gaaagacttg ctgccgttta tgcgcagcga tgctgaagcc ttcagcatgg
    36781 acaaagtgtt acgtcaggcg gttgtcgttc ctgaaagtaa gcgcgtagac cggatgctga
    36841 aagagtttcg ctctcagcgt taccacatgg cgatcgttat tgacgaattc ggtggggttt
    36901 ccggtctggt gaccattgaa gacatcctgg aactgattgt tggtgagatt gaagacgagt
    36961 atgacgaaga agatgatatc gacttccgtc agctgagtcg tcatacctgg accgtgcgcg
    37021 cactggcttc cattgaagac ttcaacgaag cgttcggcac ccactttagc gatgaagaag
    37081 tcgacactat cggtggtctg gtgatgcagg cgtttgggca tcttccggcg cgtggcgaaa
    37141 ctatcgacat cgacggttac cagttcaaag tggcgatggc cgatagtcgg cgtattattc
    37201 aggttcatgt caaaatcccg gatgactcac cccagccaaa gctggatgaa taaaaccgaa
    37261 actggataga taactacatg gcttttgcct cattaattga acgccagcgt attcgcctgc
    37321 tgctggcgtt attattcggt gcctgcggaa cgctggcctt ctctccttac gacgtctggc
    37381 ctgcggcgat tatttcgctg atggggcttc aggcgttgac ctttaaccgt cgcccactcc
    37441 agtctgccgc tattggcttt tgctggggat ttggcctctt tggcagcggt attaactggg
    37501 tctatgtcag catcgcgacc tttggcggga tgcctggccc ggttaacatc ttcctggtgg
    37561 tgctgctggc ggcgtatttg tcgctgtata ccggactgtt tgctggcgtg ctgtcgcgtc
    37621 tgtggccgaa aaccacctgg ctgcgcgtgg cgattgccac ccctgctctc tggcaagtga
    37681 ccgagtttct gcgcggttgg gtgctgactg gcttcccgtg gttacagttc ggctatagcc
    37741 agattgatgg cccgttaaaa gggctggcac cgctaatggg cgtggaagcc attaacttcc
    37801 tgctgatgat ggttagtggc ctgctggcac tggcgttggt caaacgcaac tggcgtccgc
    37861 tggtggtggc cgtcgtgctg tttgccctac ccttcccgct gcgttacatc caatggttta
    37921 ccccgcaacc ggagaaaacc attcaggttt cgatggttca gggcgatatt ccgcaatcgc
    37981 tgaaatggga cgaaggccag cttcttaata cgctaaagat ttactacaac gcaacggcac
    38041 cgctgatggg caaatcatcg ttgattatct ggccggagtc ggcgataacc gatctggaaa
    38101 ttaatcagca accgttcctc aaagcactgg acggtgagtt gcgtgataaa ggtagctcgc
    38161 tggtaaccgg gattgtcgac gcgcgtctca ataagcagaa ccgctacgat acctacaaca
    38221 ccatcatcac gctgggtaaa ggtgcaccgt acagctacga atcagccgat cgctataaca
    38281 aaaaccatct ggtgccgttt ggcgagtttg tcccgctgga gtcgattctg cgtccgttag
    38341 caccgttctt tgatctgccg atgtcgtcgt tcagccgtgg gccatatatc cagccgccgc
    38401 tgtcggcaaa tggtattgag cttactgcgg ctatttgcta cgagatcatt ctcgccgagc
    38461 aagtgcgcga taacttccgc ccggataccg actacctgct gactatctcc aacgatgcgt
    38521 ggtttggtaa atctattggt ccatggcaac acttccagat ggcgcgaatg cgtgcgctgg
    38581 agctggcgcg cccactgttg cgcagcacca acaacggcat tacggcggtg attggcccgc
    38641 agggtgagat tcaggcgatg atcccgcagt tcgcccgcga ggtgttaacc actaacgtga
    38701 cgccgaccac cggactcaca ccatacgcac gtaccggcaa ctggccgctg tgggtgctga
    38761 cggcactgtt tggttttgct gctgtgttga tgagtctgcg tcagcgacgt aaataatccc
    38821 ttcttatcgt gccggaatac atcccatatt tcggcacgct caccggatgc ggcatcagtg
    38881 cattatccgg ataacaatat tctcctatcc aactttggca catctattgc tttgttatac
    38941 aaggcaaagc ttgaaccagc atcagtacaa cccggttgca ccacagcaat ccaccggtag
    39001 caccaaaaga gtgcatttta agaattttgc ttcagaatgg tgcggtgcac ctcgcaaaaa
    39061 caaacaataa aacatcaata tctttacatt tagccaaact aaatgttacc taacaggcac
    39121 aacactgcac aataaagttg cagacgataa caacacaaac actcacaacg ggtatccatg
    39181 cgttcttaac gcagaagata aaggtgttgg atatgcaatt acgtaaacct gccacagcaa
    39241 tcctcgccct ggcgctttcc gcaggactgg cacaggcaga tgacgccgcc ccggcagcag
    39301 gcagtacgct ggacaaaatc gccaaaaacg gtgtgattgt cgtcggtcac cgtgaatctt
    39361 cagtaccttt ctcttattac gacaatcagc aaaaagtggt gggttactcg caggattact
    39421 ccaacgccat tgttgaagca gtgaaaaaga aactcaacaa accggacttg caggtaaaac
    39481 tgattccgat tacctcacaa aaccgtattc cactgctgca aaacggcact ttcgattttg
    39541 aatgtggttc taccaccaac aacgtcgaac gccaaaaaca ggcggctttc tctgacacta
    39601 ttttcgtggt cggtacgcgc ctgttgacca aaaagggcgg cgatatcaaa gattttgccg
    39661 acctgaaagg caaagccgta gtcgtcactt ccggcactac ctctgaagtt ttgctcaaca
    39721 aactgaatga agagcaaaaa atgaatatgc gcatcatcag cgccaaagat cacggtgact
    39781 ctttccgcac cctggaaagc ggtcgtgccg ttgcctttat gatggatgac gctctgctgg
    39841 ccggtgaacg tgcgaaagcg aagaaaccag acaactggga tatcgtcggc aagccgcagt
    39901 ctcaggaagc ctacggttgc atgttgcgta aagacgatcc gcagttcaaa aagctgatgg
    39961 atgacactat cgctcaggtg cagacctccg gtgaagcgga aaaatggttt gataaatggt
    40021 tcaaaaatcc aattccgccg aaaaacctga acatgaattt cgaactgtca gacgaaatga
    40081 aagcactgtt caaagaaccg aatgacaagg cactgaacta attacaagaa ccaggggcgg
    40141 aaattccagc cctctcgatt gttacgtagc acggacagac tatacgcctg atggtcgttc
    40201 cccatcgggc ctgaaaaccg caatacgctg ggtaacaatc ttcgagggta gcagttaacg
    40261 ctgctaccct cttttttctg gagtagattt atgtctatag actggaactg gggtattttt
    40321 ttacaacaag ccccgttcgg caataccacc tatcttggtt gggtctggag tggttttcag
    40381 gtgacgatcg ctttgtcgat ctgcgcctgg attatcgctt tcctcgtcgg ttcatttttc
    40441 ggcattttac gtaccgttcc aaaccgtttt ctctccggtc tgggtacgtt gtatgtcgaa
    40501 ctgttccgca acgtgccgtt gattgtgcaa ttctttacct ggtatctggt gatcccggag
    40561 ctgctgccgg agaaaatcgg catgtggttt aaggccgagc tggatcccaa tattcagttt
    40621 ttcctttcat ccatgctctg cctggggctg tttaccgccg cccgtgtttg cgaacaggta
    40681 cgcgccgcta ttcagtcgct accgcgtggg cagaagaatg ccgcgctggc gatgggcctg
    40741 acgctaccgc agacttatcg atacgtactg ctgcctaatg cttatcgcgt tatcgtcccg
    40801 ccgatgactt ccgagatgat gaacctggtg aaaaactccg ccatcgcctc gaccatcggt
    40861 ctggtggata tggcggcgca ggcgggtaaa ttgctcgact actcggctca cgcctgggag
    40921 tcgtttaccg ccatcacgct tgcgtatgtg ttgattaacg cttttatcat gctggtgatg
    40981 acgctggttg aacgtaaagt tcgcctgccc ggcaacatgg ggggcaaata atgtacgagt
    41041 ttgactggag ttccattgtt ccttccctgc catatctgct cgacgggctg gtgatcaccc
    41101 tgaaaatcac cgtcacggca gtcgtgattg gtattttgtg gggtaccatg ctggcggtga
    41161 tgcgcttgtc cagcttcgcg cctgtttcgt ggtttgccaa agcctacgtt aacgtattcc
    41221 gctcgattcc tttagtcatg gttttgctgt ggttttactt gattgtgccg ggatttctgc
    41281 aaaatgtgct gggattatcg ccaaaaaatg atattcgttt aatttcggcg atggtggcgt
    41341 tttcgatgtt tgaagcggcc tactattcag agattatccg tgccggtatt caaagtattt
    41401 cccgtggtca gtcgagcgcc gcgctggcgt tggggatgac tcactggcag tcgatgaaac
    41461 tgattattct gccgcaggct ttccgcgcga tggtgccgct gctgctcact cagggcatcg
    41521 tactgttcca ggatacctcg ctggtgtatg tgttatctct ggccgatttc ttccgtaccg
    41581 cctcaaccat tggtgagcgt gatggtacgc aggtcgagat gatcctgttt gccggatttg
    41641 tttatttcgt tattagcctt agcgcgtcgt tgttggtcag ctacttgaaa agaaggacag
    41701 cataatgatt accctgaaaa atgtttcaaa atggtatggt cactttcagg tgctgaccga
    41761 ctgctcaacc gaagtgaaaa aaggcgaagt ggtggtggtt tgcggcccgt ctggttccgg
    41821 caaatcaacg ctgattaaaa ccgtcaacgg cctcgaacca gtgcagcaag gtgaaatcac
    41881 cgtcgatggt atcgtggtta acgacaagaa aaccgatctg gcaaagctgc gctcccgcgt
    41941 cgggatggta ttccagcatt tcgagctgtt ccctcatctg tcgattatcg aaaacctgac
    42001 cctggcgcag gtgaaagtgc ttaaacgcga taaagcgccc gctcgtgaaa aagcattgaa
    42061 attgctggag cgcgtgggac tttccgcaca tgccaataaa tttccggcgc agctttccgg
    42121 cggtcagcag cagcgcgtgg caatcgctcg cgcattgtgt atggctccta ttgcgatgct
    42181 gttcgacgaa ccgacatcgg cgctggatcc ggagatgatc aacgaagtgc tggacgtgat
    42241 ggtggaactg gcgaacgaag gaatgaccat gatggtggtg acccacgaaa tgggctttgc
    42301 ccgcaaagtg gcgaatcggg tgatctttat ggacgagggt aaaattgtcg aagactcgcc
    42361 gaaagacgct ttcttcgatg atccgaaatc ggaccgcgca aaagacttcc tcgcgaaaat
    42421 cctgcattaa tcactctggc gcacataccc aattgcgcgc catctcacgc tttatcgttt
    42481 cgcccctcgt atctgttaca tcctggcgtt acccttgtcg caaagaagca cacaacaagg
    42541 agcaacaatg gcactgccaa ttctgttaga ttgcgaccca ggtcatgatg acgctatcgc
    42601 aatagttctc gcactcgcct caccagagct tgatgtcaaa gcaattacgt cttccgccgg
    42661 aaaccagaca ccagaaaaaa ccttacgcaa tgttctgcgt atgctgacct tgcttaatcg
    42721 caccgatatt ccggtggcag gcggcgcggt aaaaccgtta atgcgtgagt tgattatcgc
    42781 ggacaatgtg cacggcgaaa gcggtctcga cggcccggca ttaccggaac cgacgttcgc
    42841 accgcaaaac tgtacggcgg tggagctgat ggcgaaaacg ctgcgcgaaa gtgcggaacc
    42901 tgtcacgatt gtttctaccg gaccgcaaac taacgttgcc ttgctgctca atagccatcc
    42961 ggaactgcat agcaaaattg cccgtatcgt gattatgggc ggcgcaatgg ggcttggtaa
    43021 ctggacgcct gcggctgaat ttaatattta cgttgacccg gaagcggcag aaattgtctt
    43081 ccagtcaggg atcccggtgg tgatggccgg tctggatgtt actcataaag cgcaaatcca
    43141 cgttgaagac accgagcgtt tccgcgcgat tggcaaccct gtttcaacca ttgttgccga
    43201 actgctggat ttcttcctcg aatatcataa agacgaaaaa tggggctttg tcggcgcacc
    43261 gctgcatgac ccatgcacca tcgcctggct gttgaaaccc gagttgttta ccactgttga
    43321 gcgctgggtt ggcgtggaaa cacaggggaa atatacccag ggtatgacgg ttgttgatta
    43381 ttattatctg actggcaata aaccgaatgc cactgtaatt gtcgatgttg atcgtcaggg
    43441 ctttgttgat ttactggctg atcgtctgaa attttacgct taacactcgt cattgattaa
    43501 gatcaccacg cgttacctgg gtggtcttaa tctatttatc tctttttctt tcaagatata
    43561 aatgcaatgg ataatgcaga actcgccata ggtatcgatc tcggcactac caatagttta
    43621 attgccgtct ggaaagacgg tgccgcgcaa ttaattccaa ataagttcgg tgaatattta
    43681 acaccatcca taattagcat ggatgaaaat aatcatattt tagtcggaaa accggctgta
    43741 tcacggcgta cttcgcatcc ggataaaacg gcagcgttat ttaaacgtgc aatgggcagc
    43801 tggcggttag gcagcgacac atttaacgcg ccagaactgt cctctttggt attacgctca
    43861 ttaaaagaag atgccgaaga atttctgcaa cgtccgatta aagatgtggt gatctccgtt
    43921 ccggcttatt tcagtgatga acaacgcaag catacccgtt tagcagcgga cttagccggg
    43981 ttaaatgcgg tacgcttaat taatgaaccc acagcagctg cgatggcgta tggcctgcat
    44041 acccaacaaa atacccgttc gctggtgttt gatctcggtg gcggcacgtt tgacgttacg
    44101 gtgcttgagt acgccacgcc ggtgattgaa gttcacgcct ccgctggcga caacttcctc
    44161 ggtggcgaag attttaccca tatgttggtc gatgacgttt taaagcgcgc ggatgtcaca
    44221 aggaccacgc ttaacgagag tgaactggca gccttgtacg cctgtgtgga agcggcaaaa
    44281 tgtagcaatc aatcgccaat gcacattcgc tggtagtacc aggatgaaac gcgggaatgc
    44341 gaattttacg agaacgaact ggaagatttg tggttgccgc tgctcaatcg cttgcgagtg
    44401 ccgattgaac aggcgttgcg cgatgcgcgt ctgaagccga gtcaaatcga cagtctggtg
    44461 ctggttggcg gcgcgtcaca aatgccgctg gtgcagcgaa tcgccgtgcg tctgtttggc
    44521 aaattaccgt atcaaagtta cgatccgagc accattgtcg cgctgggcgc agcaatccag
    44581 gccgcctgcc gcttacgcag tgaagatatt gaagaagtga tcctcactga tatttgccct
    44641 tactcgttgg gcgttgaggt taaccgccag ggcgtttccg gcattttctc gccgattatt
    44701 gaacgaaaca ccactgtgcc cgtgtcgcgt gtagaaactt attcaaccat gcacccggaa
    44761 caggattcaa ttacggttaa cgtctatcag ggagaaaacc acaaagttaa aaacaacatt
    44821 ctggtggaat ccttcgatgt gccgttgaag aaaaccgggg cttatcagtc gattgatatt
    44881 cgctttagtt atgatatcaa cgggttgctt gaagttgacg tgcttctgga agacggcagc
    44941 gttaagtcca gagtgattaa ccacagcccg gtaacattga gcgcgcagca gattgaagag
    45001 agtcggacgc ggttatccgc attgaaaatt tatccgcgcg atatgctcat caatcgcacc
    45061 tttaaagcca aactggaaga gttgtgggcg cgggcgctgg gtgacgagcg agaagagatt
    45121 ggccgggtga tcaccgattt tgatgcggcg ctgcagtcaa acgatatggc ccgcgtcgat
    45181 gaggtcaggc ggcgggcgag cgtttattta gccattgaga cctcataatc acggctgccg
    45241 gatgcggcgt aaatgctgca tccggcatcg gcgcaaaaaa cacatcgtca actaaacaac
    45301 atcgcgattt tgccgagagc ataacctata accagcaccg ttaaactgat tttgataatg
    45361 ctggtaatgc tctgtggttt ttcaccctca tcattttttt cttcatggtt ttcaggtgtt
    45421 ggctcatcga catcttcatc ctccggctcg agttttactt tcgtcaacag catccgggtg
    45481 ataatggcct ctaatggatg agcatctccg ccttccagcc ttgtattaag acgggccagc
    45541 atctcttgtt tcggaaacgc ttcaagtact acctgttccg ctacccgata caaatccagt
    45601 aacggtgaat cttcttctgt ctcgctaccg actctcgatt tccaccacgc ccatgctttg
    45661 cgtttgaaga ataacgactc aataaacgat tgacctaacg gcgataattt tccgctatgt
    45721 aaaatttccg cccataacag ccgcgtccgg gcgctctcac caggaacata tttcagcggt
    45781 tgaccttgcg catctaccat gctccgcgct tcgataacca tcactaataa cgcgaaataa
    45841 tccgggcaat gacgcttgct ccactgcaac aacaaattat cagcttgtgt agatgggtaa
    45901 ctttcccagt acgcgccgag atcagcaagc aggctttccc cctcgccgca gtacaaacgt
    45961 tgtgcacact ggtaataccg ggcatcttcg ctttctggtt cttccgcttg ccactgtttg
    46021 gctacaggaa tgagttccac catacctgtt tgcaccgagc tgtaccagcg caacagtttg
    46081 cgatgtaact ttgcatcatc aggaatgaac cacggtcctt ccatcgccaa aaacgcagtg
    46141 acatagtcag gatggtaacg gcagcaacgt tccagcgcga agtaatattc aacagtctgg
    46201 ttctgtaacg cgacgggata ttccagaagg ctaaggaaat cgaaacaatc accagcctga
    46261 atggcctcaa gaaaagcctc cctttcttcc tcatccattc cttcgctgtc attaaacgaa
    46321 tgccagttca aacgctctgc caacaaagaa gcacaattta atgacagata tcgcgcttct
    46381 atagcgattg cacacagcgg ccagcgtaat tgatcgacat cctccatgtt ccaggtattt
    46441 aattgctgaa taaatttctg ccaggcggag ggctgaaaac gatcactttc tgaatccagt
    46501 aatgtcctga atgcacgtag tatttcatgt tcagcggtgg catctttttc ttcatcatct
    46561 gcttcctcga caggatttac cgccagtcgc agcgcctctt cataggcctg gcgtaatgct
    46621 ttaaacccct gcggatcggt ttcgggatga cacaacggta agcgagcaag ataagcctgg
    46681 cggataatgt ctatttgcgt cgtgctttca atttcgagga tttgccaaca tgttttcata
    46741 ggtcgctcac atccttaagc gttaccgggc attcaggctg gacagttaat ggctgccagg
    46801 gaagcgtggc atagaaacgg tcgcttcccg ctgccagctc ttcgaacatc cgactcccgg
    46861 cgctatcgta tttgtacagg gttagcgcct cgcgtagcga agcgttgtct tcgcactgag
    46921 aagattgcca gtagagacgc cctagtgaat aggcagaaaa atagtgcatc cagctgtgat
    46981 agtaatggtg agctcgtgca tagacgcgcg attgcagcca taaactctct tcttccgtca
    47041 gccagttgtt gagcacaccg gcccgtgaca gaaaccccat tcgaacataa tcccaggctt
    47101 tgattccccc ttctccacag cacatagcgg tttgggcaac gaattgggta taaatcaacc
    47161 ctctctccga gccaccagca attaatgctt tccactcttc aggggaagag cgttgccatg
    47221 tcagatataa gccagctaaa tcattggcat gaccatcatc agtcatctga taaatagtct
    47281 gaattaaatc atcacgatta tttattcccc aggatgatga gagatcgaat tcttttatcc
    47341 cggtatacat ttttggcgag tcatgcgcgg gtttgcaact ctgattaagt atttccatgg
    47401 gcgcagaaag cgcaaaaaga agatattgtt cttccttatt cattttttaa taatccctta
    47461 agtgcattaa ctgactactt ttttattatt gatgagtata aacttgacgc tttcaaaata
    47521 aaaagaaaat cgaagcattc acacatgaat aaaaaattaa tgtatatatt cgcaattttt
    47581 atagttgcag caattacctg tattagccaa cccaagaaaa cgatgttgcg tgataaagcc
    47641 atggtgaatt atgcctttga ttatttaaga tcaccaggca gtctaccgtt aaccacggca
    47701 gccacggagc tttccgcgat tcatggtcac tcaacatcgc aatatcgcct tggcgaattt
    47761 tatcttcatg gtagcgacgg taaaccactg gattatacac aggcgagata ctggtatgag
    47821 caatcagcag aacaggaaaa tccgcgcgca caaagcaaac tgaggtggat ctacctgaaa
    47881 ggcctgggag tcaaacccga tacccgtaaa gcaattctct ggtataagga agcagctgaa
    47941 caagggtatg ctcatgctca atatactttg ggtttgatct acagaaatgg cactggtatt
    48001 aatgttaacc attatgaatc tcaaaaatgg ttaaaactgg ccgccaaaca acattacaaa
    48061 aatgcggaaa gattacttgc cggacttccc gcacattaac gtcgactgtc acccgaagag
    48121 atggagtatt ttccctatca aaccggcaaa gataaaaatg tagaaaataa ttttgatgac
    48181 gcccagacag cccagcccgc catcatcaac gggcattggc tgtggttgcg gctccggctc
    48241 gacggtttcc tgaccggtaa aattcgccgc ctgaattaat aatcgacgaa taattaatgc
    48301 ttccagtgga caacgatctt ctgcatcaag gcggctcaca atcggcatta atgattcttc
    48361 tgtcggttgg ctggcctgca ctaattgttt ggcaactaaa tataatggcc ataagggatg
    48421 ttctgtatca cgcgtgagtg agatccgcgg cttacgtcct tcgtgcaaaa ccatctcgac
    48481 cagtgaacgc cccagcgcag agagtttttc atcgctgagt atttcgtata aatgaaaacg
    48541 cgtacgggcg ctgtcgccgg ggacataaag tagcggtttt ccctgatcgt taaccagatc
    48601 acgcgcttca atcatcatca ccactaatgg gtaataatag acccgatgct gacggcacca
    48661 ttgcaacatt aaagcatccg cctgggtgga gggatactcg cgccagtagt aacaaagttc
    48721 tgccagcagg ctttcgcctt ctccacaata gacgcgttgt gcgtattcat aataacgcgc
    48781 atcttcattg tcaggttcag cttcccgcca cgaccgggcg taatccagga gttcggggat
    48841 tcccaggcgg cttgcgctat accagcggag taaatttctg tgcagttttg catcatcggg
    48901 gataatcact gcgcgatgtt gcgctaaata caatgtcagc cattctggat gcgatgacca
    48961 aatccgagcg aacatttggt aaaaatcgat ggtgtcattt tgcacggcaa ctggcagatg
    49021 cagaatggtc tggaagttaa aaacattccc cttcgcaatg gcatataaaa aggattccag
    49081 ttcttcttcg tctatttccc cgacgttttc ctcctgcaac caccgcaatc tttctgctaa
    49141 taacaccacg cactcgaagg ataaatgggc agtgttcatg gcgattgtgc acagcgacca
    49201 gcgtaattca tcaatctctt ccatcgagca ataatttaat tgctgaatga atcgctgcca
    49261 ggcggaggac agaaaacgtt cgctatcaga ggcaagtaac gcgcgaaagg cgagcagaat
    49321 ttcatgttct gctacctcat attcttctgg ttgccaaaca gatttagcag gcgactgcgc
    49381 aatccgtagc gcctcctcat acgcctgacg aagttgttta aaaccctgcg gatcggtttc
    49441 tggatgaaag gacggtaaca gcgccagata agcgcggcgg ataatatcga cgtcagtcgt
    49501 ttcctctata tcgaggatct tccagcaatt tttcataagt cgctcatatc cttaagcgtt
    49561 tccgggcatg tggggtagtc agccagtggt tgccaggaca aggtagggta atacgcatca
    49621 tcactggcaa ataattgata aaacatatca ttgccggaat cgtcgtattc tttacgcgct
    49681 aagaattcgc gtaataaagg cagatgatca tcttcgggag actgccaata ttgccgacca
    49741 aaggtataac cggcaaagta ttgccgccag ttactataat aacgtagagc ccgtagatga
    49801 atacgcgact ggatccatag actttcttct tcactcaacc agttattaag tacgcccatt
    49861 cgacttaaaa aacccatccg gacataatcc catgctttaa ttcctccctc accacagcat
    49921 tcggctgtgc tggcgacaaa ttgcgcataa gcctgacctt gctcatttaa ttcagcaaga
    49981 tagtcacgcc attcacatgg agaataacga aaccagcggt gataaaaccc tgccaaccga
    50041 gcggcatgac cgttctcgat cattcggtga attatgcgca gcaggtcatc acggtcatca
    50101 attccccatg aggaagaaag gtcaaaaaag catgtaccta aatacatttt gggggatgaa
    50161 tgcgaaggta aacagcattc attgcgtatt tccatcggag tggaaagtgc aaacaatatt
    50221 ttttgcgacc ccatgtccat ataatttatc cttaaacatt catttaatga atgtaaacta
    50281 catttcgagg ctcagttatt tatacttttc tgacgcaaca taatacgcat aaaataaggc
    50341 ttacgcaaaa atggaataca taaaaatata atatatttta tctacaacca taaggaaatg
    50401 ataatgattt ttacgtcaag ttgctgcgat aatttatcaa tagatgagat tatcgaacgt
    50461 gctgaaaaag gcgattgcga ggctcaatat attgttgggt tttattataa tcgcgatagc
    50521 gcaattgact ctccggacga cgaaaaggcc ttttactggc tgaagctggc cgctgagcaa
    50581 ggtcattgtg aagcacagta ttccttaggg caaaagtata ccgaggataa aagccgtcat
    50641 aaagataatg agcaagccat cttctggctg aaaaaagctg ccctacaagg ccatactttc
    50701 gcttccaacg cccttggctg gatactggat cgtggagaag accccaacta taaagaagcg
    50761 gttacctggt atcagatagc cgcggagagc ggaatgtctt atgcgcaaaa taatcttgga
    50821 tggatgtaca gaaatggcaa cggagtcgca caagactata cgctggcatt tttctggtac
    50881 aaacaagctg cattacaagg ccatagttac gcacaagaca atctggccga tctttataaa
    50941 gacggagaag gcgttgctca aaacaagaca ctcgccgcat tctggtattt gaaaagcgca
    51001 cagcagggta atcggcacgc ccagtttcaa attgcatggg attataacgc tggcgaaggg
    51061 gtggaccagg actataagca agcgatgtac tggtatctga aggctgccgc tcaggaaagc
    51121 gtcggcgctt acgtcaacat cggttatatg tataaacacg gacaaggcgt tgagaaagat
    51181 tatcaggctg cctttgaatg gtttacgaaa gccgctgaat gcaatgacgc cactgcctgg
    51241 tataacctgg ccattatgta tcactacgga gaaggaagac ctgtcgatct ccgacaggct
    51301 ctcgacctgt atcgtaaagt tcagtcatcc ggaaccagag atgtcagtca ggaaattcgt
    51361 gagactgaag ctttactgta gaaataaaaa caagggccgg ggaatttcat tctgcccata
    51421 ttctctttat tgctgtgcga aatcagacta ttacggctca aacgggcgtc tctggaactg
    51481 gtcataacca catttcgggc atagcgtcag cacttccggt gtgtagatcg ggagatggaa
    51541 gtgacatttc tcgcagacca gatttcccag cccgaccact tctccgctgt gataaacccc
    51601 atgatgattg aggtcctgga aaatttcgcg ccattcaagc tgcgttttat cggtgatgtc
    51661 tgccagctcc tgccacaagc tttctttaat cacccgcata aagacgctgt cagattcttc
    51721 tttcaggctc tcttcatagc tcatggcgaa ctcttccagg tcacgtctga cagctcgcgt
    51781 cagctcatcg atctcggttc gcgttaactc ccctgttttt attacgcgct cgcgcgcctg
    51841 ttccaccagt gcgtcgatat cacgttcgcc attgcgcagg cgttcgttca gtgacgcgac
    51901 cagttcacgg taatattgag caaccttgtt catcgtttca tctcccgggt cgttaactga
    51961 ctataataat agacgttctt tgttccttgc tttgctaata cgcccacaaa ctctgtaaat
    52021 taatgtacga ataatccggg catcatttca acgtcgagtg cgcgaaaggc tgttttgacg
    52081 cggacgtttg ggctatgcta tgcggatctg aaaaaccacc tcaacgctac atttgtagcc
    52141 gtattgaaaa caggaccact ggctgccatg caagagcaat accgcccgga agagatagaa
    52201 tccaaagtac agcttcattg ggatgagaag cgcacatttg aagtaaccga agacgagagc
    52261 aaagagaagt attactgcct gtctatgctt ccctatcctt ctggtcgact acacatgggc
    52321 cacgtacgta actacaccat cggtgacgtg atcgcccgtt accagcgcat gctgggcaaa
    52381 aacgttctgc agccgatcgg ctgggacgcg tttggtctgc ctgcggaagg cgcggcggtg
    52441 aaaaacaaca ccgctccggc accgtggacg tacgacaaca tcgcgtatat gaaaaaccag
    52501 ctcaaaatgc tgggctttgg ttatgactgg agccgcgagc tggcaacctg tacgccggaa
    52561 tactaccgtt gggaacagaa attcttcacc gagctgtata aaaaaggcct ggtatataaa
    52621 aagacttctg cggtcaactg gtgcccgaac gaccagaccg tactggcgaa cgaacaagtt
    52681 atcgacggct gctgctggcg ctgcgatacc aaagttgaac gtaaagagat cccgcagtgg
    52741 tttatcaaaa tcactgctta cgctgacgag ctgctcaacg atctggataa actggatcac
    52801 tggccagaca ccgttaaaac catgcagcgt aactggatcg gtcgttccga aggcgtggag
    52861 atcaccttca acgttaacga ctatgacaac acgctgaccg tttacactac ccgcccggac
    52921 acctttatgg gttgtaccta cctggcggta gctgcgggtc atccgctggc gcagaaagcg
    52981 gcggaaaata atcctgaact ggcggccttt attgacgaat gccgtaacac caaagttgcc
    53041 gaagctgaaa tggcgacgat ggagaaaaaa ggcgtcgata ctggctttaa agctgttcac
    53101 ccattaacgg gcgaagaaat tcccgtttgg gcagcaaact tcgtattgat ggagtacggc
    53161 acgggcgcag ttatggcggt tccgggtcac gaccagcgcg actacgagtt tgcctctaaa
    53221 tacggcctga acatcaaacc ggttatcctg gcggctgacg gctctgagcc agatctttct
    53281 cagcaagccc tgactgaaaa aggcgtgctg ttcaactctg gcgagttcaa cggtcttgac
    53341 catgaagcgg ccttcaacgc catcgccgat aaactgactg cgatgggcgt tggcgagcgt
    53401 aaagtgaact accgcctgcg cgactggggt gtttctcgcc agcgttactg gggcgcgccg
    53461 attccgatgg tgacgctgga agacggtacc gtaatgccaa ccccggacga ccagctgccg
    53521 gtgatcctgc cggaagatgt ggtgatggac ggcattacca gcccgattaa agcagatccg
    53581 gagtgggcaa aaactaccgt taacggtatg ccagcgctgc gtgaaaccga cactttcgac
    53641 acctttatgg agtcctcctg gtactatgcg cgctacactt gcccagagta caaagaaggt
    53701 atgctggatt ccgaagcggc taactactgg ctgccggtgg atatctacat tggtggtatc
    53761 gaacacgcca ttatgcacct gctctacttc cgcttcttcc acaaactgat gcgtgatgca
    53821 ggcatggtga actctgacga accagcgaaa cagttgctgt gtcagggtat ggtgctggca
    53881 gatgccttct actatgttgg cgaaaacggc gaacgtaact gggtttcccc ggttgatgct
    53941 atcgttgaac gtgacgagaa aggccgtatc gtgaaagcga aagatgcggc aggccatgaa
    54001 ctggtttata ccggcatgag caaaatgtcc aagtcgaaga acaacggtat cgacccgcag
    54061 gtgatggttg aacgttacgg cgcggacacc gttcgtctgt ttatgatgtt tgcttctccg
    54121 gctgatatga ctctcgaatg gcaggaatcc ggtgtggaag gggctaaccg cttcctgaaa
    54181 cgtgtctgga aactggtgta cgagcacaca gcaaaaggtg atgttgcggc actgaacgtt
    54241 gatgcgctga ctgaagatca gaaagcgctg cgtcgcgatg tgcataaaac tatcgctaaa
    54301 gtgaccgatg atatcggccg tcgtcagacc ttcaacaccg caattgcggc gattatggag
    54361 ctgatgaaca aactggcgaa agcaccgacc gatggcgagc aggatcgcgc tctgatgcag
    54421 gaagcactgc aggccgttgt ccgtatgctc aacccgttca ccccgcacat ctgcttcacg
    54481 ctgtggcagg aactcaaagg cgaaggcgat atcgacaacg cgccgtggcc ggttgctgac
    54541 gaaaaagcga tggtggaaga ctccacgctg gtcgtggtgc aggttaacgg taaagtccgt
    54601 gccaaaatca ccgttccggt ggacgcaacg gaagaacagg ttcgcgaacg tgctggccag
    54661 gaacatctgg tagcaaaata tcttgatggc gttactgtac gtaaagtgat ttacgtacca
    54721 ggtaaactcc tcaatctggt cgttggctaa gcgcgggagg aagcgtgcga tatctggcaa
    54781 cactgttgtt atctctggcg gtgttaatca ccgccgggtg tggctggcat ctgcgtgaca
    54841 ccacgcaggt tccttccact atgaaggtca tgatcctgga ctcaggcgat ccgaacgggc
    54901 cattaagccg tgcggtgcgt aaccagttac gtctgaatgg tgtcgagttg cttgataaag
    54961 aaaccacgcg taaggacgtt ccatccctgc gtttgggtaa agtgagcatc gcgaaagata
    55021 ccgcatcggt attccgtaac ggtcaaacag cagagtatca gatgatcatg acggtgaatg
    55081 cgaccgtgtt gatccccggc cgtgatatct acccgattag cgccaaagtc ttccgttcat
    55141 tcttcgataa cccgcaaatg gcgttagcga aagataacga acaagacatg atcgtaaaag
    55201 agatgtacga ccgtgctgcc gaacagctga ttcgtaagct gccaagcatc cgtgctgcgg
    55261 atattcgttc cgacgaagaa cagacgtcga ccacaacgga tactccggca acgcctgcac
    55321 gcgtctccac catgctgggt aactgatgat tcggttgtac ccggaacaac tccgcgcgca
    55381 gctcaatgaa gggctgcgcg cggcgtatct tttacttggt aacgatcctc tgttattgca
    55441 ggaaagccag gacgctgttc gccaggtagc tgcggcacaa ggattcgaag aacaccacac
    55501 tttttccatt gatcccaaca ctgactggaa tgcgatcttt tcgttatgcc aggctatgag
    55561 tctgtttgcc agtcgacaaa cgctattgct gttgttacca gaaaacggac cgaatgcggc
    55621 gatcaatgaa caacttctca cactcaccgg acttctgcat gacgacctgc tattgatcgt
    55681 ccgcggtaat aaattaagca aagcgcaaga aaatgccgcc tggtttactg ctcttgcgaa
    55741 tcgcagcgtg caggtgacct gtcagacacc ggagcaggct cagcttcccc gctgggttgc
    55801 tgtgcgcgca aaacagctca acttagaact ggatgacgcg gcgaatcagg tgctctgcta
    55861 ctgttatgaa ggtaacctgc tggcgctggc tcaggcactg gagcgtttat cgctgctctg
    55921 gccagacggc aaactgacat taccgcgcgt tgaacaggcg gtgaatgatg ccgcgcattt
    55981 cacccctttt cattgggttg atgctttgtt gatgggaaaa agtaagcgcg cgttgcatat
    56041 tcttcagcaa ctgcgtctgg aaggcagcga gccggttatt ttgttgcgca cattacaacg
    56101 tgaactgttg ttactggtta acctgaaacg ccagtctgcc catacgccac tgcgtgcgtt
    56161 gtttgataag catcgggtat ggcagaaccg ccggggcatg atgggcgagg cgttaaatcg
    56221 cttaagtcag ccgcagttac gtcaggccgt gcaactcctg acacgaacgg aactcaccct
    56281 caaacaagat tacggtcagt cagtgtgggc agagctggaa gggttatctc ttctgttgtg
    56341 ccataaaccc ctggcggacg tatttatcga cggttgatat gaaatcttta caggctctgt
    56401 ttggcggcac ctttgatccg gtgcactatg gtcatctaaa acccgtggaa acgctggcga
    56461 atttgattgg tctgacgcgg gtcacaatca tccctaataa tgttcctccg catcgtcccc
    56521 agccggaagc gaacagcgtg cagcgtaaac acatgcttga actggcgatt gccgacaagc
    56581 cattatttac tcttgatgaa cgcgagctaa agcgcaatgc cccctcttac actgcgcaaa
    56641 cactgaaaga gtggcggcag gaacaaggac cggacgtgcc gctggcgttt attattggtc
    56701 aggattcatt gctgaccttt ccgacctggt acgaatacga aacgatactc gacaatgcac
    56761 atttgatcgt ctgtcggcgt ccaggttacc cacttgaaat ggcgcaaccg caataccagc
    56821 aatggctgga agatcatttg acacataacc cggaagatct tcaccttcag cctgccggta
    56881 aaatttatct ggctgaaacg ccgtggttta acatctcggc gaccatcatc cgcgaacgtt
    56941 tgcaaaacgg tgaatcgtgt gaggatttat tgccggaacc ggtattgact tacattaacc
    57001 aacaaggctt gtatcgctga tacctgtcgt tacggagggc atgatgcgac tgtggttaat
    57061 tcgtcatggc gaaacacaag cgaatgtcga tggtctgtac agcggtcatg cgcccacacc
    57121 cctgaccgca cgcggtattg agcaagcgca aaatctgcat acactgctag atgatgtttc
    57181 ctttgacctg gttttatgca gtgaactgga acgggcacag cataccgcgc gactggttct
    57241 cagtgaccgc cagctccccg ttcatatcat ccctgaactc aacgaaatgt tttttggcga
    57301 ctgggagatg cgacatcatc gcgacctcat gcaagaagat gccgaaaact atagcgcgtg
    57361 gtgcaatgac tggcagcatg ctatccccac gaatggtgaa ggatttcagg cattttcgca
    57421 acgtgtggaa cgctttatcg caaggcttag tgaatatcag cactatcaga atattttaat
    57481 cgtcagccat cagggcgtac tgagtctgtt aattgctcgt ttaattggca tgcctgccga
    57541 gtctatgtgg cattttcgcg ttgaccaggg gtgttggagc gccattgata tcaaccaaaa
    57601 attcgcgacg ctacgcgtcc tcaatagccg tgccatcggg gtcgagaacg catgactttt
    57661 ctgttttttt acgggtaagc cgcaacgacc attgacagcc ccgggcaggc tgatattctc
    57721 cgcagccaga ctttttccgc cagacacgac tttgtagaaa ttgttttaca aaaatggcga
    57781 tgcaatctgc ggcgcggggt gggatgatag cccactttcg aaagccgatt cggcgacaat
    57841 tgtcccgaaa tcgcctctgg ttcaggtata ctgacagacc atttttatct atttgattca
    57901 cccaggggga aaacttgcag ggtaaagcac tccaggattt tgttatcgac aaaattgatg
    57961 acctcaaagg tcaggacatc atcgccttag acgttcaagg caaatccagc atcaccgact
    58021 gcatgatcat ctgtacgggt acgtccagcc gtcatgttat gtccattgct gaccacgttg
    58081 tgcaggagtc tcgcgcagcg ggcctgttac cgctcggcgt agaaggtgaa aacagcgccg
    58141 actggattgt cgtggatttg ggcgatgtga ttgtccatgt catgcaggaa gagagccgtc
    58201 gcctgtatga actggaaaaa ctctggagtt aatgcgtgaa gctgcaactt gtcgccgtgg
    58261 gaacgaaaat gccggactgg gtacaaaccg gttttaccga gtacctgcgt cgttttccga
    58321 aagatatgcc cttcgagctg attgaaattc cggccggcaa acgcggcaag aatgcggaca
    58381 tcaagcgcat actcgacaaa gagggtgagc agatgttggc ggccgcaggc aaaaaccgca
    58441 ttgtcaccct cgatattcca ggcaagccct gggatacgcc gcagttagcc gctgagctgg
    58501 aacgctggaa gctggatggt cgcgacgtaa gtctactgat tggcgggcct gaagggttgt
    58561 cgcctgcctg taaagcggcg gctgagcaga gctggtcgct gtcggcgctt accctccccc
    58621 atccgctggt tcgcgtgctg gtcgcagaga gtctgtaccg ggcgtggagc atcaccacca
    58681 accatcctta tcaccgtgag tgataaggga gctttgagta gaaaacgcag cggatgaaac
    58741 tacagaactc ttttcgcgac tatacggcag agtccgcgct gtttgtgcgc cgggcgctgg
    58801 tcgccttttt ggggattttg ctgctgaccg gcgtgcttat cgccaacctg tataatctgc
    58861 aaattgttcg ctttaccgac taccagaccc gctctaatga aaaccgcatt aagctggtgc
    58921 ctatcgcgcc cagccgcggc attatctacg accgtaacgg tatccctctg gccctcaacc
    58981 gcactatcta ccagatagaa atgatgccgg agaaagtcga taacgtgcag caaacgctgg
    59041 acgctttgcg cagcgtggta gatctgaccg atgacgatat tgctgcattc cgaaaagagc
    59101 gcgcacgttc acaccgtttc acctctattc cggtgaaaac taacctaacc gaagtacaag
    59161 tagctcgctt tgccgtcaat cagtaccgtt ttccgggtgt cgaagttaaa ggctataaac
    59221 gtcgttacta tccttacggt tcggcgttga cccacgtcat cggctatgtg tcgaaaatca
    59281 acgataaaga cgtcgaacgc ctgaataatg acggcaaact ggccaactat gcggcaacgc
    59341 atgatattgg taagctgggc attgagcgtt actatgaaga cgtgctgcac ggtcagaccg
    59401 gttatgaaga ggttgaagtt aacaaccgtg ggcgtgttat ccgacagtta aaagaagtac
    59461 caccgcaagc cggacacgat atttacctga cgctggatct caaactccag caatatattg
    59521 aaacgctgct ggcgggtagc cgcgcagctg tggttgttac cgaaccgcgt acaggtgggg
    59581 tgctggcgct ggtttccacg cctagttatg acccaaacct gtttgttgac ggtatctcca
    59641 gcaaagatta ttccgccttg ctgaacgacc cgaatacacc gctggtgaac cgcgccacac
    59701 agggggttta tcctcccgcg tctacagtta aaccctatgt ggcggtttcg gcattaagcg
    59761 ccggggtgat cacgcgcaat acgacgctgt ttgacccagg ctggtggcaa ctaccagggt
    59821 cggaaaaacg ttatcgtgac tggaaaaaat ggggccacgg gcgtctgaat gtcacaagat
    59881 cgctggaaga atctgcggat accttcttct atcaggtggc ctacgatatg gggatcgatc
    59941 gcctctccga atggatgggt aaattcggtt atggtcatta caccggtatc gacctggcgg
    60001 aagaacgttc cggcaacatg cctacccgcg aatggaaaca gaaacgcttt aaaaaaccgt
    60061 ggtatcaggg tgacaccatt ccggttggta tcggccaggg ttactggaca gcgaccccaa
    60121 tccagatgag taaggcactg atgatcctga ttaatgacgg tatcgtgaag gttccgcatt
    60181 tgctgatgag caccgccgaa gacggcaaac aggtgccatg ggtacaaccg catgaaccgc
    60241 ccgtcggcga tattcattcc ggttactggg agctggcgaa agacggtatg tacggtgttg
    60301 ctaaccgccc taatggtacg gcgaataaat actttgccag cgcaccgtac aaaattgcgg
    60361 cgaaatctgg taccgctcag gtcttcggtc tgaaagcgaa cgaaacctat aatgcgcaca
    60421 aaattgccga gcgtttacgt gaccacaaac tgatgaccgc ctttgcgcca tacaacaatc
    60481 cgcaagtggc tgtcgccatg attctggaga acggcggcgc gggtccggcg gttggtacgc
    60541 tgatgcgcca gatcctcgac cacattatgc tgggtgataa caacaccgat ctgcctgcgg
    60601 aaaatccagc ggttgccgca gcggaggacc attaatcatg acggataatc cgaataaaaa
    60661 aacattctgg gataaagtcc atctcgatcc cacaatgctg ctgattttac tggcattgct
    60721 ggtttacagc gccctggtta tctggagcgc cagcggtcag gatattggca tgatggagcg
    60781 taaaatcggc caaatcgcga tgggtctggt catcatggtg gtgatggcgc aaattcctcc
    60841 acgcgtttat gaaggctggg ccccctatct ctatatcatc tgtattattt tgctggtggc
    60901 ggtagatgct ttcggagcca tctctaaagg cgctcaacgc tggctggacc tcggtattgt
    60961 tcgttttcag ccgtcggaaa ttgccaaaat agccgtacca ctgatggttg cgcgctttat
    61021 caaccgcgac gtttgcccgc catcgttgaa gaatactggc atcgcgctgg tgctgatatt
    61081 tatgcccacg ctgctggtgg ctgcacagcc tgacctggga acatcaatcc tcgttgcgct
    61141 ttccggtctg tttgtactgt tcctctctgg ccttagctgg cgtctgattg gcgtcgcagt
    61201 agtgctggta gcggcgttca ttccgattct gtggttcttc ctgatgcatg attaccagcg
    61261 ccagcgcgta atgatgctcc tggacccgga atcagaccca ctcggcgcgg gctatcacat
    61321 tattcagtct aaaattgcta ttggctccgg cggattacgc ggcaaaggct ggctgcacgg
    61381 cactcagtca cagcttgaat ttctccccga acgccatact gactttatct tcgcggtact
    61441 ggcggaagag ctgggattag tgggcattct gattctgctc gctctctaca ttctgctgat
    61501 catgcgcggg ctgtggatag ccgccagagc gcaaaccacc tttggtcgcg tcatggctgg
    61561 tggcttaatg ctgatattat tcgtttatgt cttcgtaaat attggtatgg taagcggtat
    61621 tctgccggtt gtaggggttc cgctcccact ggtcagttat ggaggatcgg cgctaattgt
    61681 gctgatggct gggttcggga ttgtaatgtc aatccacacc cacaggaaaa tgttgtcgaa
    61741 aagcgtgtaa gaggtgcgca atgcgtaagc agtggctcgg gatctgcatc gcggcaggaa
    61801 tgctcgcggc atgtacaagc gatgatggtc agcaacagac ggtaagtgta ccgcagcctg
    61861 cggtatgtaa cggccccata gttgaaatta gcggggcgga cccgcgtttc gaaccactga
    61921 acgcgacggc aaatcaggat taccagcgcg acggtaaaag ctacaaaatc gtgcaggatc
    61981 cgtctcgatt tagccaggcg ggactggcgg caatctatga tgccgaacca ggcagtaacc
    62041 tgacggcctc tggcgaagct ttcgatccga caaagctgac ggcggcccat ccaacgcttc
    62101 cgatccccag ctacgccaga atcactaacc tggctaacgg gcgaatgatc gtggtgcgca
    62161 ttaatgatcg cggcccttac ggcaacgacc gcgttatttc gctttctcgc gcggcagctg
    62221 accgtcttaa cacgtcaaac aacaccaaag ttcgtatcga tccgattatt gtcgcccagg
    62281 atggttcgct ttctggtcct ggtatggctt gtaccacagt cgccaaacag acttacgccc
    62341 tgcctgcacc tcccgattta agcggtggcg cgggaacaag ttcagtgtct ggcccgcagg
    62401 gtgacattct tccggtcagt aattcgacgc taaaaagcga agatccgacc ggcgcgccgg
    62461 taaccagtag cggtttcctc ggcgctccaa cgaccttagc gcctagtgta ctggaaggca
    62521 gcgaaccgac gcctgcgcca cagcccgttg ttacagcttc gtcgacaacg cctgcaacct
    62581 cgcctgcaat ggtgacaccg caagccgcct cgcaaagcgc cagcggcaac tttatggtgc
    62641 aagtcggggc cgtaagcgat caggctcgtg cgcaacagta ccaacagcaa ctgggacaga
    62701 agttcggcgt ccccggtcgc gtaactcaaa atggcgcggt ctggcggatc cagcttggcc
    62761 cattcgccag caaagccgaa gccagtacct tgcagcaacg tttgcaaacc gaagcccaat
    62821 tacagtcatt tattactacc gcgcagtagc gtaaagcagg catctgatgt gtcaatttct
    62881 gtaaatgacg ttaacaaagt cacgcggaaa gtcagatgcc tgccggtagt gcatttgcta
    62941 tagtagggca cttttttaat tccatcacgg atgtcgtagt tcagaccatg aataccattt
    63001 tttccgctcg tatcatgaag cgcctggcgc tcaccacggc tctttgcaca gcctttatct
    63061 ctgctgcaca tgccgatgac ctgaatatca aaactatgat cccgggtgta ccgcagatcg
    63121 atgcggagtc ctacatcctg attgactata actccggcaa agtgctcgcc gaacagaacg
    63181 cagatgtccg ccgcgatcct gccagcctga ccaaaatgat gaccagttac gttatcggcc
    63241 aggcaatgaa agccggtaaa tttaaagaaa ctgatttagt cactatcggc aacgacgcat
    63301 gggccaccgg taacccggtg tttaaaggtt cttcgctgat gttcctcaaa ccgggcatgc
    63361 aggttccggt ttctcagctg atccgcggta ttaacctgca atcgggtaac gatgcttgtg
    63421 tcgccatggc cgattttgcc gctggtagcc aggacgcttt tgttggcttg atgaacagct
    63481 acgttaacgc actgggcctg aaaaataccc acttccagac ggtacatggt ctggatgctg
    63541 atggtcagta cagctccgcg cgagatatgg cgctgatcgg ccaggcattg atccgtgacg
    63601 taccgaatga atactcgatc tataaagaaa aagaatttac gtttaacggt attcgccagc
    63661 tgaaccgtaa cggcctgtta tgggataaca gcctgaatgt cgacggcatc aaaaccggac
    63721 acactgacaa agcaggttac aatcttgttg cttctgcgac tgaaggccag atgcgcttga
    63781 tttctgcggt gatgggtgga cgtactttta aaggccgtga agccgaaagt aaaaaactgc
    63841 taacctgggg cttccgtttc ttcgaaaccg ttaacccact gaaagtaggt aaagagttcg
    63901 cctctgaacc ggtttggttt agtgattctg atcgcgcttc gttaggggtt gataaagacg
    63961 tgtacctgac cattccgcgt ggtcgcatga aagatctgaa agccagctat gtgctgaaca
    64021 gcagtgaatt gcatgcgccg ctgcaaaaga atcaggtcgt cggaactatc aacttccagc
    64081 ttgatggcaa aacgatcgag caacgcccgc tggttgtgtt gcaagaaatc ccggaaggta
    64141 acttcttcgg caaaatcatt gattacatta aattaatgtt ccatcactgg tttggttaaa
    64201 aattgaacac ttgaaagtgt aatttccgtc cccatatact aagcatcagt aaaaaaactc
    64261 ccgccttctg gcgggagttg ctatttaatt acgttacgcc ggagctgaca tgaaaaccaa
    64321 acttaacgaa ctgcttgaat tccctactcc ttttacttac aaagttatgg ggcaggcgtt
    64381 acctgagctg gttgatcagg tggttgaagt ggtacagcgc catgcgccag gtgactacac
    64441 cccaacggta aaaccaagca gcaaaggcaa ctaccactcg gtatctatca ctatcaacgc
    64501 cactcatatc gagcaggttg aaacactgta tgaagaactg ggcaaaatcg atattgtccg
    64561 catggttctg taactcgctt ctccgttacc cggctcctgg tcgggtaact cccctctcct
    64621 cgctgtgata tacttttccc ccacttttac tcattctcca cggagatgcc gttttgtatc
    64681 aggataaaat tcttgtccgc cagctcggtc ttcagcctta cgagccaatc tcccgggcta
    64741 tgcatgaatt caccgatacc cgcgataata gtacccttga tgaaatctgg ctggtcgagc
    64801 actatccggt attcacccaa ggtcaggcag gaaaagcgga gcacatttta atgccgggtg
    64861 atattccggt gatccagagc gatcgcggtg ggcaggtgac ttatcacggg ccgggacaac
    64921 aggtgatgta tgtgttgctt aacctgaaac gccgtaaact cggtgtgcgt gaactggtga
    64981 ccttgcttga gcaaacagtg gtgaataccc tggctgaact gggtatagaa gcgcatcctc
    65041 gggctgacgc gcctggtgtc tatgtcgggg aaaagaaaat ttgctcactg ggtttacgaa
    65101 ttcgacgcgg ttgttcattc cacggtctgg cattaaacgt caatatggat ctttcaccat
    65161 ttttacgtat taatccttgt gggtatgccg gaatggaaat ggctaaaata tcacaatgga
    65221 aacccgaagc gacgactaat aatattgctc cacgtttact ggaaaatatt ttagcgctac
    65281 taaacaatcc ggacttcgaa tatattaccg cttaattcca tacatcaatg gcccaattta
    65341 cactgggtca ttactcataa tacctgctaa tgctttacat ctccttcgcg tttgtatatt
    65401 ttctttatca cttgcagaaa tgcggtgcca cgccttgctc cgtaactcac cccattgatt
    65461 aacctattga aatagttgat tatggtgagc aaaagcgttc cgttccaatt agacataaga
    65521 ttattgtaat tttttaaata ttattttccc atgaataaaa ttggagtaag tgcgtggata
    65581 gtaataatca aattgaaccc tgtttaagca gaaagtcgtc agaaggtaaa ccacaaatat
    65641 ttacaacctt acgaaatatt gatcttaacc ttctgactat ttttgaagct gtatatgtac
    65701 ataaagggat cgttaatgca gcgaaagtgc ttaatctgac cccctcggca atcagtcagt
    65761 ctattcagaa actgcgcgtt atattccctg acccattgtt tattcgcaaa ggccagggtg
    65821 tcactcctac cgcatttgcg atgcatctac atgagtatat cagtcagggc cttgagtcca
    65881 ttcttggcgc gctggatatc gaaggaagct atgataagca acgaacgata actattgcga
    65941 ccactccctc ggtcggagcc ctggtccttc ctgtcatcta tcgggcgatt aaaactcact
    66001 atccgcagct tttactgcgc aacccgccca tcagcgacgc ggaaaaccaa ctcagtcagt
    66061 ttcaaaccga tctcatcatc gataacatgt tttgcaccaa tcgtacggtg caacatcatg
    66121 ttctgttcac cgacaatatg gtgttaattt gccgcaaggg aaatccacta ctctctttag
    66181 aagatgacag agagactatc gacaacgctg cgcatgtact tctgttaccg gaagggcaaa
    66241 atttcagcgg tctgcggcag agagttcaag agatgtttcc ggaccggcaa atcaatttca
    66301 ccagctacaa cattttgaca atcgctgcac tggttgccaa cagtgacatg ttagcgatta
    66361 ttcccagccg tttttataac ctgtttagtc gttgctggcc gctggaaaaa ctgccttttc
    66421 cgtccttaaa tgaggagcaa atagatttct ctatccacta caacaaattc atcctgcgtg
    66481 atcccatcct gcacggagta atagatgtta tccgtaatgc attttgaaaa agtattgtta
    66541 cagccagatc acagcgcaga acgataaaat ggcacaatcc aacaacaatt ttacattttc
    66601 gcgaccgctt tggctgcttt caggtccgtt tcaatgatat actgccagtc gttaattcaa
    66661 aaatagttga taattacaac aatctattga attgaaacgc tttccttcgt aattcgcaac
    66721 tggaacacgc acgctatgag taaacccatt gtgatggaac gcggtgttaa ataccgcgat
    66781 gccgataaga tggcccttat cccggttaaa aacgtggcaa cagagcgcga agccctgctg
    66841 cgcaagccgg aatggatgaa aatcaagctt ccagcggact ctacacgtat ccagggcatc
    66901 aaagccgcaa tgcgcaaaaa tggcctgcat tctgtctgcg aggaagcctc ctgccctaac
    66961 ctggcggaat gcttcaacca cggcacagca acgtttatga tcctcggcgc tatttgtacc
    67021 cgccgttgtc cgttctgtga tgttgcccac ggtcgcccgg tagctcctga tgccaatgaa
    67081 ccagtgaaac tggcgcagac cattgccgat atggcgctgc gttatgtggt tatcacctcc
    67141 gttgaccgtg atgacctgcg cgatggcggt gcccaacact ttgcggattg tattactgcc
    67201 attcgggaaa aaagcccgca aatcaaaatt gaaactctgg tgccggattt ccgcggtcga
    67261 atggatcgtg ctctggatat tctgactgca acgcccccag atgtgttcaa ccataacctg
    67321 gaaaacgtac cgcgtattta ccggcaggta cggcctggcg cagattacaa ctggtcgctg
    67381 aagctgctgg aacgctttaa agaagcgcat ccggaaatcc cgaccaagtc tggtctgatg
    67441 gtgggtctgg gtgaaaccaa tgaagaaatt attgaggtaa tgcgcgacct gcgccgtcat
    67501 ggtgtgacga tgttaacgct ggggcaatat ttgcagccaa gccgccatca cctgccggtt
    67561 caacgttacg ttagcccgga tgagttcgac gaaatgaaag ccgaagcgct ggcgatgggc
    67621 tttacccatg ctgcatgcgg tccgtttgtc cgctcttctt accacgccga tttgcaggcg
    67681 aaaggaatgg aagttaagta atcctgtaat aattacttac atctgtctga taaaaaaccc
    67741 gctttttgaa gcgggttttt tgtatcgaac aagatattga gggagcgtcc tgctcgccac
    67801 gtcactcttt atgagagagc ttttcagcct gaagatcaac gtctgcgcct tttttcgccg
    67861 cagcatcgtc atcattcatc gccttcttga accctttaat ggccgctcca aggtctccgc
    67921 ccagcgtacg taacttctta gtcccaaaca gcagaacgac cagcgccgca actaccagca
    67981 gtttggtaat actaatctca cccatagata ccttcttgac atataaccgg caacagaacg
    68041 cgccttaaaa accagagaat attaacggtc attcgacgcg cgcacacaat agcaatctgt
    68101 aacaaggtga atcaagcgcc gagtaaaaaa acatcataat aattgcggcg gcgcaaagcg
    68161 acggttgttt aagatgggca gttgcgcgcg cacctgacgc acacgttcgg gcgtcacttc
    68221 cgccataatg agtgcaggca tttctgacgc tgccgcaatg gtgacgccaa agggatctat
    68281 aatccggctt tgcccgatat ttttgttccc gcactccccc gccgccacca tataacaggt
    68341 ggtatccagc gcacgagcgg caagcaacgt tgaccagtga tgctctttga gcggcccacg
    68401 aacccaggcg gcaggaagta ccaggatttc agctccctgt aaagcctgtg ccagcgccag
    68461 ctctggaaag cgtaagtcat aacaggtcat cagaccgacc ttcatccctt ccacctccag
    68521 taacggagct atttcattac cagcatcaac atggcgtgat tcctgaatgg caaatgcatc
    68581 atagagatgc agtttggcat aacgggcgac gatgtttcct gcctgaagtg ccaccagcat
    68641 attccatgcg cgccccggcg ttgaaggaag atgaatcgtc agaattgtcg tcatcatgtt
    68701 acgtttactt tctcgccgta aacgtccgag gaattcgcct tccagcagct gtgctgattt
    68761 aaccgataga tctgcatcat gatcatcgcg cgccagcaat gcttccggca ggacaaacag
    68821 cgatacgtcg ttttccgccg cctgcgccat caacgaggca caaatctcag cgttcttttc
    68881 ccacacagat gtaacagcaa actgtcctgc agcaaccagc attgccacct cctgtcagag
    68941 tcgtaaaatt cgccaacgac gattattcgg cgttacactt atcactcata caaatcaaat
    69001 agcaggattt tgcagtgtta caacttcttt tagcagtttt tattggcggt ggtacgggaa
    69061 gcgtagcgag atggctgtta agtatgcgat ttaacccgct gcatcaggcg attccgttgg
    69121 ggacgctggc agcaaacctg attggggcat tcatcatagg aatgggattc gcctggttca
    69181 gcaggatgac gaacattgat ccagtgtgga aagtattaat caccaccgga ttttgtggcg
    69241 gtctaacaac cttctcaaca ttttcggcag aagtggtgtt tttgttacaa gagggccgct
    69301 ttggctgggc attactgaac gttttcgtca accttctggg gtcttttgcc atgaccgcac
    69361 tggcattctg gctgttttcg gcctcaaccg tacactaaag accaaaaaaa acccgctgat
    69421 taagcgggtt ttgaattctt gctgacgtat cttacagagc gattacgttt gcagcagaag
    69481 ggcctttggc accgttagtg atttcgaact ctacgcgctg accttcagca agagttttaa
    69541 aaccattagt ctggattgca gagaagtgta cgaacacgtc tttgctgccg tcttccggag
    69601 taatgaaacc gaatcctttg gactcattaa accacttaac gttaccttta atcttagaca
    69661 tcaaaattac ctttacatga aaaatagaca caaatgctgt gtcggttatc agtacaccaa
    69721 ttgtggtacg ctttgtccag tgcaacttta ctaaaaagtg ataaaagtcg cgttattttt
    69781 gatgttgctt attttagtta ttgattttaa agaagttact aaaacttcat ttgtctcaaa
    69841 actgaaagcg catccaggca aagtacacat tgccattgtt gtaggtaccc ggaatgtagg
    69901 tcatctgaaa agtcactggg ccataaccca cggaggccaa tggcagtaga accgggagag
    69961 ggatgtaatt ccagttatcg cgtgccgtta cgccagcggt gaatcccaga cctaaatgaa
    70021 aattttcatc cgccagcggt cgccaggtac tttcccatcc gtatccggca atcggttccc
    70081 atttgttcca cgagtcctta aatgccatgg catacaggcc atgccagttt cctttttcat
    70141 cccaacgcga caggccaaaa ccgccacccc acggtcgctc gttatagcga tcggtttttt
    70201 ctttgtcgta agcgaaacgt gcatgccagg tgatggcagg aatatataaa tcataatgtt
    70261 caggctgttg ccaggtttgt gcaatatttt ctctaaacgt tgtcatcctc tcatctgcgt
    70321 tagcaaaaac tttaccaacg ctgattaact gaataaaaac aaaggaaaag atagcgacat
    70381 atttactcac gttcatttgt gaccataaaa catttatcaa aaatctacta ctagcatagc
    70441 aaagctacaa ttaacataac cttaatagac ccaacataaa gaataatctg aatagctgct
    70501 ctatttattt ccgcttgttc aagttaaata tttccgcgca tcgattaaag atgatgcgtc
    70561 gttttctgcg atgggaatag tcaaaaaaga aaaaccaagt cttttttgat gacaaaatgc
    70621 aatcaaggaa aaattatttt atttttttaa cgagatacaa caatcatctt aacgaagtat
    70681 ataatatata tcgtcaacca tgctcatatt agattatttt ttgagcgcac agccgcagct
    70741 aacaccattg caattaacaa atttgcatca atccaccatc aatttacaca cattattatg
    70801 tgataattgc caaccgctaa atatgcgttt tgttatctat gtataaaaac agcaacttca
    70861 atgtcttaat ggcagttttt cttgatttta atcagcattc atcgccaatt tattgggcat
    70921 attttttcct taagctttag gaagttttta ttatttactt tgggcctgga gacaggaaaa
    70981 attatgctga cattcattga actccttatt ggggttgtgg ttattgtggg tgtggctcgc
    71041 tacatcatta aagggtattc cgccactggt gtgttatttg tcggtggcct gttattgctg
    71101 attatcagtg ccattatggg gcacaaagtg ttaccgtcca gccaggcttc aacaggctac
    71161 agcgccacgg atatcgttga atacgttaaa atattactaa tgagccgcgg cggcgacctc
    71221 ggcatgatga ttatgatgct gtgtggcttt gccgcttaca tgacccatat cggcgcgaat
    71281 gatatggtgg tcaagctggc gtcaaaacca ttgcagtata ttaactcccc ttacctgctg
    71341 atgattgccg cctattttgt tgcttgtctg atgtcactgg ccgtctcttc cgcaaccggt
    71401 ctgggtgttt tgctgatggc aaccctgttt ccggtgatgg taaacgttgg tatcagtcgt
    71461 ggcgcagcag ctgccatttg tgcctccccg gcggcgatta ttctcgcacc gacttcaggg
    71521 gatgtggtgc tggcggcgca ggcttccgaa atgtcgctga ttgacttcgc cttcaaaacg
    71581 acgctgccta tctcaattgc tgcaattatc ggcatggcga tcgcccactt cttctggcaa
    71641 cgttatctgg ataaaaaaga gcacatctct catgaaatgt tagatgtcag tgaaatcacc
    71701 accactgccc ccgcgtttta tgccattttg ccgttcacgc cgatcatcgg tgtactgatt
    71761 tttgacggta aatggggtcc gcaattacac atcatcacta ttctggtgat ttgtatgctg
    71821 attgcctcca ttctggagtt cctccgcagc tttaataccc agaaagtttt ctctggtctg
    71881 gaagtggctt atcgcgggat ggcagatgcg tttgctaacg tggtgatgct gctggttgcc
    71941 gctggggtat tcgctcaggg gcttagcacc atcggcttta ttcaaagtct gatttctatc
    72001 gctacctcgt ttggttcggc gagtatcatc ctgatgctgg tattggtgat tctgaccatg
    72061 ctggcggcag tcacgaccgg ttcaggcaat gcgccgtttt atgcgtttgt tgagatgatc
    72121 ccgaaactgg cgcactcttc cggcattaac ccggcgtatt tgactatccc gatgctgcag
    72181 gcgtcaaacc tggatcgtac cctatcaccc gtttctggcg tagtcgttgc ggttgccggg
    72241 atggcgaaaa tctcaccatt tgaagtcgta aaacgcacct cggtgccggt gcttgttggg
    72301 ctagtgattg ttatcgttgc tacagagctg atggtgccag gaacggcagc agcggtcaca
    72361 gacaagtaaa tagtaatgcc ggaggcaagt ttcctccggc tttatcatgt cacccactgt
    72421 ggtatatgcg ttgtggtctg ccaactttgc cgtgaacaat ttcagcaata atcagatggc
    72481 ggctggcgca atattcaaga taacgcctgg cagtggtgcg gctgatggtc agtgcctgcg
    72541 ccaccgtttc tgccgtatgt tgcacaccag gctctttaaa cagttttcgc accgcgttta
    72601 gcgtcaaggg gtcaatgccg gtcggcagtt cgtctttggg ttcaccgcga gcataggcat
    72661 taaacatctc atcaatttgc ttctggctgg cgctatcaat actttccagc atatgtttac
    72721 gctggcggaa acgggttagc gtttgcccca gtgtattgat tcacttgaag tacgaaaaaa
    72781 ccgggagaac attggattat tcgggatctg atgggattag atttggtggg gcttgcaagc
    72841 ctgtagtaca aattttaggc gttaaccaat gaaacgcgaa agatagtaaa aaattgcttt
    72901 tgtttcattg aaaaaacgaa aaacaaaaac actataaatc atttcaatca tagcttcaaa
    72961 aaacgttcaa aaccgataac aaccaggctg tcaccaaatg actcatatca caaatcagct
    73021 catgccgttt aggtatgtta catgtgtgat tatgtgaggt gaagtatgtt ttagctggct
    73081 catggttgtt atacggcttt tttcgcatct tgtggttcca acaaaggagc tacaggctct
    73141 tcctgtttat gaacaccata cattgacaaa atctcttggc gagtttctgg aggtaattta
    73201 tgcagcgcca aagctgtttg tagtgtttgg cgatcaatac ccagcgttgc caatagggtg
    73261 tcaacggaga tggaggggcg ttcttgcatt aatccggaaa gcccatattt agccatgttt
    73321 agctctatct ctcggacttc attttctcct agtttattga ttaacaggga gaacattttc
    73381 aatgtatcag cattcatttc ttcgatagca attttttgca tagccggtat tgccgggata
    73441 tattcaaccc caggcccgcg acgcccttct tgtgaacgct tcggccatcc ttctttcttt
    73501 gctcgtttat gcaccccctg tattgtccca ggcattccat ccagttcagt aatttgtttg
    73561 ggtgtcatcc actggtttat ctcatttgcg cttttcataa actaagttta ccagatttat
    73621 ctatataaaa atcaatggat tacaaaataa accacattaa ctgatataaa ccggttttgt
    73681 ttatttggta atgtataaaa cagataatca agacgaatta tttgttttgg taacccttta
    73741 tcatacgcaa tatggttggt gtgcgaaaga tgagtaacga ttggcatcgg gccgacgtga
    73801 ttgcggccct caaaaaaaca ggaacccctc tggcaaaatt gtccagacgt tcaggttatt
    73861 caaccgggac gcttgcaaat gcattagtca ggccatggcc taaaggtgaa cgcattattg
    73921 ctgacgcctt aaacatgaaa cctgaggaaa tttggcctag tagatatcta agggatcaca
    73981 atgagagagt ggatatcagc aattgaatgc atgggtttat ctggtttacc tggttcatta
    74041 atgggggttc acaaaagagc tcagagagaa ggatggctca agagaagaag agaaggaatt
    74101 agaggggttg cttacgaata ccatatatct tctcttccta acattgctcg agctgaagtt
    74161 ttgctccaac aaggaaaatt taaaaccagt cagggatgtt ttgaaatcgc ccgccccacg
    74221 ctggaagctc atgattatga tcgtgaggca ctatggagca aatgggataa agccagcgat
    74281 tcccagcgca gcctggctga aaaatggttg ccctcaattc aggccacaga cgaaatgctg
    74341 aaccagggga tttcaacgaa aacggctttt gcgaccgttg cagggcatta ccaggtcagc
    74401 gcatccactt tgcgggacaa gtattaccag gtacagaagt ttgcgaagcc tgactgggcg
    74461 gctgcacttg ttgatggacg tggagcatcc cgtcgcaatg ttcacaaaag tgaatttgac
    74521 gaggatgcct ggcagtttct gattgcagat tatctgcgac cggaaaaacc cgccttccgc
    74581 aaatgttatg agcgtctgga actggcagcc cgcgagcatg gctggagtat tccctcccgt
    74641 gccacggcct ttcgccggat tcagcaactg gacgaggcaa tggttgttgc ctgtcgtgaa
    74701 ggtgaacatg cactgatgca tctgataccg gcacagcagc gaactgtgga acacctggac
    74761 gccatgcagt ggatcaacgg cgacggttat ctgcataacg tctttgtacg ctggtttaac
    74821 ggtgatgtga tccgtccgaa aacatggttc tggcaggatg tgaaaacccg aaaaattctg
    74881 ggctggcgct gcgatgtgag cgagaacatt gattcaattc gcctctcgtt catggatgtt
    74941 gtgactcgct acagtatccc ggaggatttt cacatcacca ttgataacac ccgtggtgct
    75001 gcgaataaat ggctgacggg aggcgcgccc aatcgctacc gctttaaggt aaaagaggac
    75061 gatccaaaag gactgttttt actgatgggt gcgaaaatgc actggacaag cgttgttgcc
    75121 ggtaaaggct ggggccaggc aaaacctgtt gaacgtgctt tcggtgttgg tgggcttgag
    75181 gaatacgttg ataagcatcc ggcactggct ggcgcatata cggggccaaa tccgcaggca
    75241 aaacctgata actatggcga ccacgctgtt gatgcagagc tgtttctgaa aacctttgcc
    75301 gaaggtgtgg cgatgttcaa tgccagaaca ggccgtgaaa cagaaatgtg cgggggcaaa
    75361 ctctcgtttg atgatgtttt cgagcgtgaa tacgccagaa cgattgtgcg taagccaacc
    75421 gaagaacaaa aacggatgct gttactgcct gccgaggcgg tgaacgtttc acgcaaaggc
    75481 gagtttacgc ttaaagttgg cggctccctt aaaggcgcga aaaacgttta ttacaacatg
    75541 gcattaatga atgccggagt gaaaaaagtt gtggtcagat ttgatccaca gcagttacac
    75601 agcacggttt attgag
//
LOCUS       DAJTQY010000005        72925 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.005, whole genome shotgun sequence.
ACCESSION   DAJTQY010000005 DAJTQY010000000
VERSION     DAJTQY010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 72925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72925
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.005"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OO349_000322"
     CDS             complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OO349_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR5865526.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(280..2394)
                     /gene="fusA"
                     /locus_tag="OO349_000323"
     CDS             complement(280..2394)
                     /gene="fusA"
                     /locus_tag="OO349_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCR5865527.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRVGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="OO349_000324"
     CDS             complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="OO349_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HCR5865528.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="OO349_000325"
     CDS             complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="OO349_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HCR5865529.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="OO349_000326"
     CDS             complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="OO349_000326"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HCR5865530.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTVQILNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="OO349_000327"
     CDS             complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="OO349_000327"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HCR5865531.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDNLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLSLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="OO349_000328"
     CDS             complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="OO349_000328"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HCR5865532.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHKLSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4598..5320)
                     /locus_tag="OO349_000329"
     CDS             complement(4598..5320)
                     /locus_tag="OO349_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR5865533.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="OO349_000330"
     CDS             complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="OO349_000330"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR5865534.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKASFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6520..6738
                     /locus_tag="OO349_000331"
     CDS             6520..6738
                     /locus_tag="OO349_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HCR5865535.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="OO349_000332"
     CDS             complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="OO349_000332"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR5865536.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7472..7672)
                     /locus_tag="OO349_000333"
     CDS             complement(7472..7672)
                     /locus_tag="OO349_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HCR5865537.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVHKDEQVIGIFHPD"
     gene            complement(7682..9419)
                     /gene="kefB"
                     /locus_tag="OO349_000334"
                     /pseudo
     CDS             complement(7682..9419)
                     /gene="kefB"
                     /locus_tag="OO349_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
     gene            complement(9419..9970)
                     /gene="kefG"
                     /locus_tag="OO349_000335"
     CDS             complement(9419..9970)
                     /gene="kefG"
                     /locus_tag="OO349_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HCR5865538.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     KELASHARAYGDWLANPLSPGGR"
     gene            10101..12014
                     /locus_tag="OO349_000336"
     CDS             10101..12014
                     /locus_tag="OO349_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5865539.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHSKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFLGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12014..13036
                     /locus_tag="OO349_000337"
     CDS             12014..13036
                     /locus_tag="OO349_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR5865540.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEDPAQAKHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPSQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13030..13248
                     /locus_tag="OO349_000338"
     CDS             13030..13248
                     /locus_tag="OO349_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HCR5865541.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13302..14171
                     /locus_tag="OO349_000339"
     CDS             13302..14171
                     /locus_tag="OO349_000339"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HCR5865542.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14226..14630)
                     /locus_tag="OO349_000340"
     CDS             complement(14226..14630)
                     /locus_tag="OO349_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HCR5865543.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            14932..15564
                     /gene="crp"
                     /locus_tag="OO349_000341"
     CDS             14932..15564
                     /gene="crp"
                     /locus_tag="OO349_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HCR5865544.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15615..17705
                     /locus_tag="OO349_000342"
     CDS             15615..17705
                     /locus_tag="OO349_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR5865545.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKHLIIGASLFATCSLLTQLLLVKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTVLHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPESYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17772..18992)
                     /gene="argD/dapC"
                     /locus_tag="OO349_000343"
     CDS             complement(17772..18992)
                     /gene="argD/dapC"
                     /locus_tag="OO349_000343"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HCR5865546.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYRYYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPHYKGRARDFLYAGAEVGVMVLNAGPDVMRLAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19078..19641)
                     /gene="pabA"
                     /locus_tag="OO349_000344"
     CDS             complement(19078..19641)
                     /gene="pabA"
                     /locus_tag="OO349_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HCR5865547.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHL"
     gene            complement(19673..20275)
                     /locus_tag="OO349_000345"
     CDS             complement(19673..20275)
                     /locus_tag="OO349_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HCR5865548.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20265..20432)
                     /locus_tag="OO349_000346"
     CDS             complement(20265..20432)
                     /locus_tag="OO349_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HCR5865549.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20537..21118)
                     /gene="ppiA"
                     /locus_tag="OO349_000347"
                     /gene_synonym="rotA"
     CDS             complement(20537..21118)
                     /gene="ppiA"
                     /locus_tag="OO349_000347"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HCR5865550.1"
                     /translation="MFKSTLAAMAAVFALSALSALSPAAMAAKGDPHVLLTTSAGNIE
                     LELDKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKN
                     EADNGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMD
                     VADKISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21389..22570
                     /gene="tsgA"
                     /locus_tag="OO349_000348"
     CDS             21389..22570
                     /gene="tsgA"
                     /locus_tag="OO349_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HCR5865551.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKRAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPVHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22832..25375
                     /gene="nirB"
                     /locus_tag="OO349_000349"
     CDS             22832..25375
                     /gene="nirB"
                     /locus_tag="OO349_000349"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HCR5865552.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25372..25698
                     /gene="nirD"
                     /locus_tag="OO349_000350"
     CDS             25372..25698
                     /gene="nirD"
                     /locus_tag="OO349_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HCR5865553.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDKQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            25824..26630
                     /gene="nirC"
                     /locus_tag="OO349_000351"
     CDS             25824..26630
                     /gene="nirC"
                     /locus_tag="OO349_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HCR5865554.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26649..28022
                     /gene="cobA"
                     /locus_tag="OO349_000352"
     CDS             26649..28022
                     /gene="cobA"
                     /locus_tag="OO349_000352"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCR5865555.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            28269..28436
                     /locus_tag="OO349_000353"
     CDS             28269..28436
                     /locus_tag="OO349_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HCR5865556.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            28731..30068
                     /gene="frlA"
                     /locus_tag="OO349_000354"
                     /pseudo
     CDS             28731..30068
                     /gene="frlA"
                     /locus_tag="OO349_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
     gene            30089..31111
                     /gene="frlB"
                     /locus_tag="OO349_000355"
     CDS             30089..31111
                     /gene="frlB"
                     /locus_tag="OO349_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HCR5865557.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            31161..31991
                     /gene="frlC"
                     /locus_tag="OO349_000356"
     CDS             31161..31991
                     /gene="frlC"
                     /locus_tag="OO349_000356"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HCR5865558.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAIDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYCTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            31988..32773
                     /gene="frlD"
                     /locus_tag="OO349_000357"
     CDS             31988..32773
                     /gene="frlD"
                     /locus_tag="OO349_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HCR5865559.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            32873..33604
                     /gene="phoB"
                     /locus_tag="OO349_000358"
     CDS             32873..33604
                     /gene="phoB"
                     /locus_tag="OO349_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator PhoB"
                     /protein_id="HCR5865560.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKSTK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(33764..34849)
                     /locus_tag="OO349_000359"
     CDS             complement(33764..34849)
                     /locus_tag="OO349_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCR5865561.1"
                     /translation="MKTFPLQSLTLAEAQQKQFALVDTICRHFPGSEFLAGGDLGLTP
                     SLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITADFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPGGVGAVVGDADVISRIRA
                     TLYSGGSQIQGSQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVAEVKSAVIANA
                     QSKVLIVEFHQLIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(34861..36165)
                     /locus_tag="OO349_000360"
     CDS             complement(34861..36165)
                     /locus_tag="OO349_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HCR5865562.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIHPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(36177..36530)
                     /locus_tag="OO349_000361"
     CDS             complement(36177..36530)
                     /locus_tag="OO349_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HCR5865563.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGCFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(36541..37419)
                     /locus_tag="OO349_000362"
     CDS             complement(36541..37419)
                     /locus_tag="OO349_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HCR5865564.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            complement(37416..38642)
                     /locus_tag="OO349_000363"
     CDS             complement(37416..38642)
                     /locus_tag="OO349_000363"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCR5865565.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINTLGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVERRGDDLQFLSVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVRDQVQVGRVITFGGLLTNSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICINIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(38643..39806)
                     /locus_tag="OO349_000364"
     CDS             complement(38643..39806)
                     /locus_tag="OO349_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HCR5865566.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQILENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQ
                     GHLVQIPCHQVADAVEQGTDIITVFTLDKAREVSAAAVKAGRVQPVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVIAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNVPSATSCTSLPLLAEYGATHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEYPVSSAVVLCFRTQIFVTRSDVVLVSGIQRGEPEIVGRYDSLGNPLEA"
     gene            complement(39890..40252)
                     /locus_tag="OO349_000365"
     CDS             complement(39890..40252)
                     /locus_tag="OO349_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR5865567.1"
                     /translation="METRLNLLCEAGVIDKDVCKGMMQVVNVLEKECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQELLKEFALEVNPCEE
                     GYFLANLYGLWMAANEEV"
     gene            complement(40269..41174)
                     /locus_tag="OO349_000366"
     CDS             complement(40269..41174)
                     /locus_tag="OO349_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709156.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5865568.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLSQNNLCIALE
                     LGPHTYVGEHQLICRKGESGNVKRVGLDSRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(41444..42448)
                     /gene="trpS"
                     /locus_tag="OO349_000367"
     CDS             complement(41444..42448)
                     /gene="trpS"
                     /locus_tag="OO349_000367"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HCR5865569.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCVYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFGYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(42441..43199)
                     /gene="gph"
                     /locus_tag="OO349_000368"
     CDS             complement(42441..43199)
                     /gene="gph"
                     /locus_tag="OO349_000368"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HCR5865570.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(43192..43869)
                     /gene="rpe"
                     /locus_tag="OO349_000369"
     CDS             complement(43192..43869)
                     /gene="rpe"
                     /locus_tag="OO349_000369"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HCR5865571.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(43887..44723)
                     /gene="dam"
                     /locus_tag="OO349_000370"
     CDS             complement(43887..44723)
                     /gene="dam"
                     /locus_tag="OO349_000370"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCR5865572.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMERADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(44830..46116)
                     /gene="damX"
                     /locus_tag="OO349_000371"
     CDS             complement(44830..46116)
                     /gene="damX"
                     /locus_tag="OO349_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HCR5865573.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTTSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPVATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDSAKTQTAERPSTTRPVRQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(46208..47296)
                     /gene="aroB"
                     /locus_tag="OO349_000372"
     CDS             complement(46208..47296)
                     /gene="aroB"
                     /locus_tag="OO349_000372"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HCR5865574.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTSET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(47353..47874)
                     /gene="aroK"
                     /locus_tag="OO349_000373"
     CDS             complement(47353..47874)
                     /gene="aroK"
                     /locus_tag="OO349_000373"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HCR5865575.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(48275..49513)
                     /gene="hofQ"
                     /locus_tag="OO349_000374"
     CDS             complement(48275..49513)
                     /gene="hofQ"
                     /locus_tag="OO349_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HCR5865576.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNDNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QESEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(49425..49829)
                     /gene="hofP"
                     /locus_tag="OO349_000375"
     CDS             complement(49425..49829)
                     /gene="hofP"
                     /locus_tag="OO349_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HCR5865577.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKSPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            complement(49819..50259)
                     /gene="hofO"
                     /locus_tag="OO349_000376"
     CDS             complement(49819..50259)
                     /gene="hofO"
                     /locus_tag="OO349_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HCR5865578.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLEMPHEG"
     gene            complement(50243..50782)
                     /gene="hofN"
                     /locus_tag="OO349_000377"
     CDS             complement(50243..50782)
                     /gene="hofN"
                     /locus_tag="OO349_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HCR5865579.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(50782..51561)
                     /gene="hofM"
                     /locus_tag="OO349_000378"
     CDS             complement(50782..51561)
                     /gene="hofM"
                     /locus_tag="OO349_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HCR5865580.1"
                     /translation="MTFKIWQIGLHLQQQEAAAVAIVRGAKECFLQRWWRLPLAHDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALSVDPDS
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            51681..54233
                     /gene="mrcA"
                     /locus_tag="OO349_000379"
     CDS             51681..54233
                     /gene="mrcA"
                     /locus_tag="OO349_000379"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HCR5865581.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVVTLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTMMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQGATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(54398..54958)
                     /gene="nudE"
                     /locus_tag="OO349_000380"
     CDS             complement(54398..54958)
                     /gene="nudE"
                     /locus_tag="OO349_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HCR5865582.1"
                     /translation="MNKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            55278..57413
                     /gene="igaA"
                     /locus_tag="OO349_000381"
     CDS             55278..57413
                     /gene="igaA"
                     /locus_tag="OO349_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HCR5865583.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            57478..58146
                     /gene="yrfG"
                     /locus_tag="OO349_000382"
     CDS             57478..58146
                     /gene="yrfG"
                     /locus_tag="OO349_000382"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HCR5865584.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            58157..58558
                     /gene="hslR"
                     /locus_tag="OO349_000383"
     CDS             58157..58558
                     /gene="hslR"
                     /locus_tag="OO349_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HCR5865585.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            58583..59452
                     /gene="hslO"
                     /locus_tag="OO349_000384"
     CDS             58583..59452
                     /gene="hslO"
                     /locus_tag="OO349_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HCR5865586.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNTQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADP"
     gene            complement(59596..61320)
                     /locus_tag="OO349_000385"
     CDS             complement(59596..61320)
                     /locus_tag="OO349_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HCR5865587.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNPWRAEKAIWD
                     FGCYLLLMAMLLLPWIQQSLRIRNGSSRYSYFYQSVWHNVLILLVIFLSNGLTWLVLL
                     LWGELFKLVGITFFNTLFFATDWFMYLTFGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTPMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVATWGLWLRVAQYGWTVDRLQGALAELVLLVWSLGY
                     FVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQEQVSEKV
                     FAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKAWRDLTVDGE
                     TLEINLSK"
     gene            61699..63321
                     /gene="pckA"
                     /locus_tag="OO349_000386"
     CDS             61699..63321
                     /gene="pckA"
                     /locus_tag="OO349_000386"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HCR5865588.1"
                     /translation="MRVNNGLPPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(63397..64749)
                     /gene="envZ"
                     /locus_tag="OO349_000387"
     CDS             complement(63397..64749)
                     /gene="envZ"
                     /locus_tag="OO349_000387"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HCR5865589.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(64746..65465)
                     /gene="ompR"
                     /locus_tag="OO349_000388"
     CDS             complement(64746..65465)
                     /gene="ompR"
                     /locus_tag="OO349_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HCR5865590.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            65693..66169
                     /gene="greB"
                     /locus_tag="OO349_000389"
     CDS             65693..66169
                     /gene="greB"
                     /locus_tag="OO349_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HCR5865591.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCLENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEANWYVNAIEYVKP"
     gene            66266..68587
                     /locus_tag="OO349_000390"
     CDS             66266..68587
                     /locus_tag="OO349_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HCR5865592.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     SVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNSRRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            69025..69252
                     /gene="feoA"
                     /locus_tag="OO349_000391"
     CDS             69025..69252
                     /gene="feoA"
                     /locus_tag="OO349_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HCR5865593.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFDVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            69269..71590
                     /gene="feoB"
                     /locus_tag="OO349_000392"
     CDS             69269..71590
                     /gene="feoB"
                     /locus_tag="OO349_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HCR5865594.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDATLARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFNVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGNGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVAIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            71590..71826
                     /gene="feoC"
                     /locus_tag="OO349_000393"
     CDS             71590..71826
                     /gene="feoC"
                     /locus_tag="OO349_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HCR5865595.1"
                     /translation="MASLIQVRDLLALRGRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            72029..72916
                     /gene="rpnA"
                     /locus_tag="OO349_000394"
     CDS             72029..72916
                     /gene="rpnA"
                     /locus_tag="OO349_000394"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HCR5865596.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESRSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADI
                     MRFTGLSEEELAAASRLAP"
BASE COUNT        17572 a        19760 c        18450 g        17143 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcacgagc agcaccgccg taggttttag ccagtacggt ggtgattgca
      121 gcggtcagag tagttttacc gtggtcaacg tggccgatag taccaacgtt aacgtgcggt
      181 tttgtacgtt caaatttttc tttagacacg gctatattcc ttactatagt gctctcccct
      241 tcaggagaga gcacgggact ttggtattaa cccttaggct tatttaccac gggcttcaat
      301 tacggcctga gcaacgttac tcggcgcttc atcatacttc aggaattcca tagtgtatga
      361 tgcacgacct ttggtcagag aacgcagctg agttgcgtat ccgaacattt cagacagcgg
      421 tacttcagcg tggatcttaa cgccagtaac ttcagattcc tgacctttga gcataccacg
      481 acgacggctc aagtcaccga taacgtcacc ggtgttctct tccggagttt ctacttcaac
      541 cttcatgatc ggctcaagca gaactggttt cgctttctta aagccttctt taaaggcgat
      601 agaagcagcc agtttaaacg ccagttcaga ggagtcaacg tcatggtaag aaccgaagtg
      661 cagacgaata cccatgtcta ctaccgggta gcctgccagc ggacctgctt tcagctgttc
      721 ctggatacct ttatcaacgg ccgggatgta ttcgccaggg attacaccac ctttaatgtc
      781 gttgatgaac tcgtagcctt tcgggtttga acccggctcc agcgggtaca tgtcgataac
      841 aacatgacca tactgaccac gaccaccaga ctgtttcgcg tgtttacctt caacatcggt
      901 aactttctgg cggatagttt cacggtaagc aacctgcggt ttacctacgt tcgcttcaac
      961 gttgaattca cgcttcatac ggtcaacgat gatgtcgagg tgcagttcgc ccatacccgc
     1021 gatgatggtc tggttagatt cttcgtcagt ccatacacgg aaagacgggt cttctttagc
     1081 cagacggccc agagccagac ccattttttc ctggtcagct ttggttttcg gttcaactgc
     1141 gatggagatt accggctcag ggaattccat acgttccaga atgatcggcg catccgggtc
     1201 acacagggtg tcaccagtgg ttacgtcttt cagaccgata gcagcagcga tgtcgcccgc
     1261 gcgaacttct ttgatctctt cacgtttgtt agcgtgcatc tgaacgatac gaccgaaacg
     1321 ctcacgtgca gctttcacgg agttcagtac ggtatcacca gagttaacca caccggagta
     1381 aacacggaag aaggtcaggt taccaacaaa cgggtcggta gcgattttga acgccagtgc
     1441 agagaacggc tcgtcatcac ttgcgtgacg ttcagccgga gtgtctttac cgtcgtccag
     1501 gataccgttg atcgcaggta cgtcaaccgg ggatggcagg taatcaatta ccgcatccag
     1561 catcgcctga acacctttgt tcttgaacgc agaaccacag gttaccagga tgatttcgtt
     1621 gttcagaacg cgctgacgca gagcaccttt gatttctgct tcagtcagtt cttcaccacc
     1681 caggtatttt tccatcagct cttcagaagc ttcagctgcg gattcgatca ggttctggtg
     1741 ccattcgtta gccagttcaa ccatgtctgc cgggatatct tcgtattcga aggttacgcc
     1801 ctggtcagcg tcgttccagt tgatagcttt cattttcacc aggtcaacaa caccggtgaa
     1861 atgttcttca gcaccaatcg ccagctgcag cggaaccggg ttcgcgccca gacgggtttt
     1921 gatctggtta acaactttca ggaagttcgc acccacgcgg tccattttgt taacgaacgc
     1981 aatgcgcgga actttatatt tgtttgcctg acgccatacg gtttcagact gcggctgaac
     2041 accaccaact gcgcagtaaa ccattaccgc accatcgaga acacgcatgg aacgttctac
     2101 ttcgattgtg aagtcaacgt gccccggggt gtcgatgatg ttgatgcgat gcggctcata
     2161 ctgcttagcc ataccagacc agaatgcagt agtcgcagcg gaagtgatgg taataccacg
     2221 ttcctgctcc tgctccatcc agtccatggt tgcagcgccg tcatgaactt caccgatttt
     2281 atggtttaca ccggtgtaga acagaatacg ttcggtagta gtggttttac cggcgtcgat
     2341 gtgcgcactg ataccgatgt tacggtagcg tgcgatgggt gttgtacgag ccatttgttt
     2401 cctcgtttat cttttaggcg ttcaatttaa gtagcccaaa gcgggctgct tactggaagc
     2461 gcccgcctgg tgactaaaac tccgaaggga ttaccaacgg tagtgtgcga acgccttgtt
     2521 ggcttcggcc atacggtgaa cgtcttcacg tttcttaact gcagtacctt tgttttctgc
     2581 agcatcagaa agttcgttcg ccaggcgcag agccatggat ttatcaccgc gtttacgagc
     2641 agcttcaacg atccaacgca ttgccagagc attacgacga accggacgga cttcaactgg
     2701 tacctgataa gtagaaccac caacgcggcg agacttaact tctacagtcg ggcgcacgtt
     2761 ttcgagagct acttcgaatg cttccagttc agatttacca gagcgctgag ccagggtctc
     2821 cagcgcgctg tatacgatag attcagcagt agatttttta ccatctacca tcaggatatt
     2881 tacaaattta gccagcagtt ctgatccgaa cttcggatcc ggcagaattt tacgctgacc
     2941 aatgacgcga cgacgtggca tggaaatact ccgttgttaa ttcaggattg tccaaaactc
     3001 tacgagttta gtttgacatt taagttaaaa cgtttggcct tacttaacgg agaaccatta
     3061 agccttagga cgcttcacgc catacttgga acgagcctgc ttacggtctt taacgccgga
     3121 gcagtcaagc gcaccacgta cggtgtggta acgaacaccc gggaggtctt taacacgacc
     3181 gccacggatc aggatcacgg agtgctcctg caggttgtga ccttcaccac cgatgtagga
     3241 agtcacttcg aaaccgttag tcagacgaac acggcatact ttacgcagcg cggagttcgg
     3301 ttttttagga gtggtagtat atacacgagt acatacgcca cgtttttgcg ggcatgcttc
     3361 cagcgcaggc acgttgcttt tcgcaacttt gcgagcacgt ggtttgcgta ccagctggtt
     3421 aactgttgcc attaaatagc tcctggtttt agcttttgct tcgtaaacac gtaataaaac
     3481 gtcctcacac aatatgagga cgccgaattt tagggcgatg ccgaaaaggt gtcaagaaat
     3541 atacaacgat cccgccatca ccaggccatc tggctggagt gcttaaccgt aagtctgacg
     3601 aaatcagtat agtcaatggg aatgatgtcg ttcaaaattt gaacagtcaa accgcgggca
     3661 ataaggtctt cgttcagggc atagacctta atgggggcat tacgcagact ttcaaggtag
     3721 cggttaccgt caactgcggc agttacgcca tcttgcaata atagcagttc gtctccttca
     3781 ctgagcagac gcagcagcgc agcaaaatcc gtcagccagg gtgaacgatg taacgtgtgc
     3841 agcataaacg cctcaaaacc tcaaaataac atcgtagttg gcgagttcgc ggcgtaaggc
     3901 atctgcttcg agcggcgtgg cttcgacaac aaagggtgtc tgcggatcta acccgcgttc
     3961 gcgcagtgaa gccgcacaaa cccagcactg ttcaatgtcg tacagactca acaatttaaa
     4021 agtggcaatg taatcacgcg ccagcactgc atcgggcttt tgtcctgaca gcagctgaaa
     4081 aacgccatca gcaataaaga agacggccag attgtcagtt aatgcggaag ttgccagtaa
     4141 agcatctaaa ccttcccgac cagcagctgt accatgaggt gcagtagaaa aaacaaacgc
     4201 aattcgtttc atcagaactg taccaccctg tcacaggtca gcgaggcttc cgccagcgcg
     4261 ccaagtccgc ttaaggtaaa tcccggctga aggtttgacg aagccagccc cagccttcct
     4321 gcttcagttt catcgacgat accgcggcgt aacgctgccg ctacgcagat attcagcgcc
     4381 acaccatgtt gcgcattcaa ctgttgccag ccgcgcacaa ggtcaaattc gtcgcttgcc
     4441 ggtgaggtca gttggttagc gttatagacc ccttcccgat agaaaaagac gctgcttaac
     4501 ttatggcctt ctgctatcag cgcctgcgca aactgaaaag cactactcgc ctgttgcgta
     4561 ccgtatgctg gcccggtcac cacgatggca aaacgcatta cttatcttgc ccctgaaaat
     4621 caccgctctt gaactggcgg atgtagagat agacagtgtg tttggagatg ttcaggcggt
     4681 cagcaacctg gttgatcgca tctttaatat cgaagatccc tttctcgtag agattcagca
     4741 cgatctgacg atttttggcg ttattagaaa cattgcgatc ggcattcact tcttcgatgg
     4801 tgaactccag cgtttgggta accagatctt caacagaaga ggcaaagttg acgcttgaac
     4861 cgacatccgg ggtttctggc ggcacaaaag tgctcataat ctgcgagaag ggaacatcaa
     4921 gattcatatt gatgcacagc agaccaatta cacgctgttc gcggttacga atcgcgatag
     4981 tcagggactt cattaatacg ccgcttttgg cgcgagtaaa gtagcattta gaaacgctgc
     5041 tatccgctcc cgtcatatcg tgcagcatac gtagcgccag gtcagtaatt ggcgaaccaa
     5101 tcttccggcc tgtatgttca ccgttagcaa tgcgaatggc tgaacatttt agatcctgca
     5161 aagagtgcaa aacgatttca cagtgggagc caataagcat cgctaacccg tccaccaccg
     5221 cttcgtagga tttcagaata tcaaaatcgg tctggtcgaa aggacgttga tccagtaaat
     5281 ccaactcact ggtttcgttg gttaaaagcg acctggacat gaaaaaaaac actccttttc
     5341 aggagcctgt cgttaacttt tcagggcagg ctcattaatg atgcgggtaa ctaaattaat
     5401 acagcggagg ttccgctttc cagcactaat tatatccggc ctgtaataaa aaaaccgccg
     5461 cctggtcagg cggcggttct taatgcttat tttttagcag aatctgcggc tttcgcatca
     5521 gcttccggct ttgcatcagc cttcggcgct ggtttcacat ccagcagctc tacgtcaaac
     5581 actagggtag aattcggtgg gatccccgga acacccgctt tgccgtaagc cagttctggt
     5641 ggaataacca gtttgatctt accgcctttc ttgatgttct tcagaccttc tgtccaaccc
     5701 gggataacac cgtccagacg gaaagaaagc ggttcaccac gggtgtaaga gttgtcgaac
     5761 tctttaccgt cgatcagcgt acctttgtag ttcactacaa cagtatcgct gtctttcggt
     5821 gcttcgcctt taccggcttc tactacctga taaaccagac cagttgaaga ggttttcaca
     5881 cctttctctt tggcaaattt ctcgcggtac tctttacctt ttgcttcgtt atcagccgcg
     5941 tctttttcca tcttcgcctg agcagaagac ttcacgcgag cttcgaatgc ttgcagagtc
     6001 tgttcgatct cttggtcgga gagtttgctc ttatcagcaa atgcatcctg aacaccagcg
     6061 atcagctgat ctttatccag tttgatgccc agtttttctt gttctttaag agagttttcc
     6121 atgtaacgac ccagcgaagc acccagtgca taagctgatt tctgatcgtc atttttgaac
     6181 gatgctttgc tgtctgcagt tgtagcaggt tttgcagctt cagcagcaaa agtgattggt
     6241 gcatgcaggg caacggccat tgtggtcgcc agtagcgtta ctttaaacag tgatttcatc
     6301 catatctcca ggatcggggc atctcacccc agggttaact attatcagaa gggtactata
     6361 aagcgttgtc gaacaaatct acatacagac acgccctatt atcatctatt ttcagactct
     6421 ttttgtttaa attagtttcg atgaccgcga aatgagtgct gtctcgggca gcaaagttaa
     6481 gtagaatccg cggcgaccat tcgacaaaag aggtgaatca tgcaggattt atcattggaa
     6541 gcacgcctgg cagagctgga gagccgactg gcttttcagg aaatcaccat tgaagaactg
     6601 aacgtcacgg tgaccgctca tgaaatggag atggcgaaac tgcgcgatca tctgcgtctg
     6661 ctgaccgaga agttaaaagc cagccagccg tcgaacatcg cgtcgcaggc tgaagaaacg
     6721 ccaccgccac attattgagg cgtaaaaaaa gcggggattc cccgcttttt tgtcaccttt
     6781 tcggtattag tggcaaccac aaccgccgtt gcctttaccg ccacagcagc cttcgccacc
     6841 gtgttcatga ccgtgatcgt ggccatgacc gccgcagcaa ccgtcgtggt cgtgatcgtg
     6901 gtgatgatcg tgcgcgccgt gaacgtggcc atgagccagt tcttcttcgg tcgcttcgcg
     6961 aatcgccaca acttcaacgt tgaatttcag gttctgaccg gccagcatgt ggttaccatc
     7021 aaccactacg tgatcgtctt caaccgcagt gatttcaacc ggtaccggac cctggtcagt
     7081 ttcagccagg aaacgcatac ctacctgcag ttcatcaacg cccataaata cgtctttagg
     7141 aacacgttgc actaggtttt cgtcgtactg accgtaagcg tcgttcgcgc caacagcgac
     7201 atcaaatttg tcgccaactt catgaccttc cagcgccgtt tccaggccag agatcaggga
     7261 accgtgacca tgcaggtagt ccagcggcgc actcaccgga gactcatcaa ccaacacacc
     7321 gtcttctgta cgtacctgat aggccaggct gaccaccagg tcttttgcta ctttcatgat
     7381 atctcctgag catgggaaga atagtggcgc agattgtagc ggaattctgc agccgtgtac
     7441 tcactagctt aaaaaaactc ggcgcatatc gctagtccgg atgaaaaatc ccgatcactt
     7501 gctcatcttt gtgaacgtga tcgcgggctt ctttgtctgc ttctcgcatc tgatgtccgc
     7561 acttaacaca ttcaacaata tcaatattat tttcgcgcca catcgccatt gaatcctgcg
     7621 cctgacaggc cgggcatttt gcgcccgcta taaaacgttt tcggattgcc atccttaccc
     7681 tctactcaaa ttcatcccag ccgtccagct ggcgtcgttc ttgttgcatt tcacgctgga
     7741 aaatctcttc cagttcgcgt cgggcttccc tggcgcgaga aatttgtacg gtatcagcat
     7801 gcatcgggat taactcccgc agcattcgca tatccaggcg gcgaaaatgc agttgcgccc
     7861 gctgtgcctg atgcggatgc atgccaagcg tgaccagcgt cttgcgcccc agctctaacg
     7921 cactggagaa tgtttcacgg gaaaactgcg tcacccctgc ctgtaataac tcatgcgctt
     7981 ccacacgtcc gcgcgctcgc gcaagaatat gcaaatgcgg aaagtgctgc tggcatattt
     8041 ccaccagctt catggtgtct tccggctcgt tacaggtaat gacgatagac tcagcggcct
     8101 ctgcacccgc agaacgtaaa agatcgacct gcgtggcgtc gccgtaataa actttgtagc
     8161 catatttacg catcaggtta acggcgctga tatcccgctc cagcacggta atgcgcattt
     8221 tatttgccat cagcaaacga ccaatcacct gaccaaaacg cccgaagccc acgacaatga
     8281 cctggggttt atcatcgttg acccacggtt tttcgtcttc ttcttccggt ccgttaaact
     8341 ggcgggatag ccatttatcc accagcttca tcagcaacgg cgccatctgg tcgccctgga
     8401 ataaacgttg tgaagaagcg gtagaaaaga ggacaaaggc aaactcaccg ccctgactca
     8461 acacgccagc aaactgcatc cgctctgagc tacgcacgcc atacaatcgc gccagcagat
     8521 acagcacgag aattttcacc gccaccagca caaccacgct tatcactacc cacaacagat
     8581 gggtataaag caccccgagg ttgagcgaca tgccgacaga gataaagaac aaaccgagca
     8641 gcaagccttt gaagggatcg atagccgttt ccagttcatg gcgatattca ctttccgcca
     8701 gcagcacgcc cgcaataaat gtaccgagcg ccatcgacag ccccagcgca tccataaaca
     8761 atgcggaacc caacaccagc agcagcgtcg cggcggtgaa cacttcccgc acgccagaag
     8821 ctgcaataaa gcggaatacc ggacgcagta aatagcgccc accaatcagc atgccgacaa
     8881 actccagcac cttcatgccg atcttcatcc agtcgaaatg ttcgtctgcc gaccccgcca
     8941 acaacggcac taacgccagt gctgggatca ctgccagatc ctgaaacagc agaaccgaaa
     9001 acccgagctg gccggattcg ctgcgattca tccctttctc acgcatcaat tgcaacgcca
     9061 ttgcagttga agacatcgca aagccaatgc caccgaccac cgccgcctgc caggcgaaat
     9121 ccgtcagcat caataatccc gccagcaacg ccgcgcttaa cagcacctgt gccgcgccta
     9181 cgccaaaaat cgaacgccgc agttgccaaa gtttggaggg attcaactca aggccgatga
     9241 taaacatcag gaataccacg ccgagttccg aaaagtggag gatctcatcg acgtcgctaa
     9301 taaaccccag cccccacggg ccaattgcaa tccccgccag caaatatccc aacacagcgc
     9361 caatccccag ccgcgaagag aaacagcact cctgcgagta aaaaatcgga accttccatc
     9421 agcggcctcc tggagacagc ggatttgcca accagtcacc gtaggctctg gcgtggctcg
     9481 ccagctcctt tgcgctttgc cgtctcgccc agtaaataat gatgggactt aaccaatgca
     9541 tccggcacat gcccgccgcc agttcaaagg ggcgcagcac atcgctcatc gggtagcgat
     9601 tcagcgcgtc ataacggtaa gcactttccg gctcgccggt ggtaatcacg ttacgccagt
     9661 actttcccgc cagttggttt cctcccggcc cgctggcaaa accacgactt aatacccggt
     9721 ccagccactc tttcagtagc gccgggcagc tataggtata aagaggatgc tgaaagacaa
     9781 tcacctcgtg ctcgcgcagt aatgcctgct cacgggggat atcaataaaa aaatcgggat
     9841 agtgcgcgta aaggtcgtgc acggtaacat tgctgagctg cgtggccggt ttaagcagta
     9901 cccggtttgc caccgagtcc tgagattccg gatgggcata cagcagcaaa actttcgctg
     9961 gctgagacat catccccctc ccggtatggt ttttgtgtat agtcgctgtt ttgggctacc
    10021 attgcgcccg gtgcggcagc tcgcccatac attacattat cataatgata agttaacata
    10081 gtctgaacat acggcgcctt atgattgttt tctcctcgtt acaaattcgt cgcggcgtgc
    10141 gcgtcctgct ggataatgcc accgccacca tcaaccccgg gcagaaagtc ggcctggtgg
    10201 gtaaaaacgg ctgtggtaaa tctaccctgc tggcattgct gaaaaatgaa atcagcgccg
    10261 acggcggcag ctacaccttt ccgggaagct ggcaactggc gtgggtgaat caggaaacgc
    10321 cagcgttacc gcaagcggcg ctggaatatg tcattgacgg cgaccgtgaa tatcgtcaac
    10381 tggaagcgca gctgcacgac gccaacgaac gtaacgacgg gcacgccatt gcgaccattc
    10441 atagcaagct ggatgctatt gacgcatgga gtattcgctc ccgtgccgcc agcctgctgc
    10501 acggcctcgg tttcagcaat gaacaactgg agcgcccggt aagtgatttc tccggtggct
    10561 ggcgtatgcg tcttaacctt gcccaggcgc tgatttgccg ttcagacttg ctgctgctcg
    10621 acgaaccgac taaccacctc gatctcgatg ccgttatctg gctggaaaaa tggttgaaga
    10681 gctatcaggg cacgctgatc ctgatctctc acgaccgcga cttcctcgat ccgatcgttg
    10741 ataaaattat tcatatcgaa caacaaagca tgttcgagta caccggcaac tacagttcgt
    10801 ttgaagtaca gcgcgccacc cgtctggcgc agcaacaagc gatgtacgaa agccagcagg
    10861 aacgcgtggc acatctgcaa agttatatcg atcgtttccg tgccaaagcc accaaagcga
    10921 agcaggccca gagccgcatt aagatgctgg aacgtatgga gctgattgcc cccgcacacg
    10981 tcgacaaccc gttccgcttt agcttccgcg cgccggaaag cctgccaaat ccgttactga
    11041 agatggaaaa ggtcagcgca ggctatggcg atcgcattat tctcgactcg attaaattga
    11101 atctggtccc cggctcgcgc attggtctgt taggccgcaa cggcgcaggt aaatcgacat
    11161 taatcaaact gttagccggt gaacttgcgc cagtcagcgg tgaaattggt ctggcgaaag
    11221 ggatcaagct cggctacttc gcccagcatc aacttgaata cctgcgcgcc gacgaatcac
    11281 ctattcaaca tctggcacgt ttagcgccgc aggagctgga acaaaaactg cgtgactacc
    11341 tcggcggctt tggtttcctg ggcgataaag taaccgaaga aacgcgccgc ttctccggtg
    11401 gggaaaaagc ccgcctggtg ctggcattaa ttgtctggca gcgtccgaat ctgctgctgc
    11461 tcgacgaacc gactaaccac cttgacctcg acatgcgtca ggcactcacc gaagcattaa
    11521 tcgagtttga aggcgcgctg gttgtcgttt cgcacgaccg tcatttgctg cgttccacca
    11581 ctgacgatct ctacctggtt cacgatcgta aagtcgaacc gttcgacggc gatctggaag
    11641 attatcaaca gtggttgagc gacgtacaaa agcaggaaaa ccagaccgac gaagcgccaa
    11701 aagagaacgc gaacagcgcc caggcacgta aagatcagaa gcgtcgggaa gcggagctgc
    11761 gtgcgcaaac ccagccgctg cgtaaagaga ttgcccgtct ggaaaaagag atggagaagc
    11821 tgaacgcgca actggcgcag gcggaagaga aactcggcga cagcgaactg tatgaccaga
    11881 gccgtaaagc ggagttgacc gcctgcctgc aacagcaagc cagcgccaaa tccggcctgg
    11941 aagagtgcga aatggcgtgg ctggaagccc aggagcagct tgagcagatg ctgctggaag
    12001 gccaaagcaa ctgatggcgc agataacgac gaccgatgcc aatgaattta gcagcagtgc
    12061 tgaattcacc cctatgcgcg gctttagcaa ttgtcatcta caaactatgc tgccgcgttt
    12121 gtttcgtcgt caggtgaaat tcaccccgta ctggcagcgg ctggagttgc ccgacggcga
    12181 ttttgtcgat ctcgcgtgga gtgaagaccc cgcacaggcg aaacataaac cgcgtttagt
    12241 ggtgtttcac gggctggaag gcagtctcaa tagcccttac gcccacggtc tggttgaggc
    12301 ggcgcaaaag cgcggctggc tgggcgtggt gatgcatttt cgcggatgca gcggtgaacc
    12361 aaaccgtatg caccgcattt accattcggg cgaaaccgaa gacgccagtt ggtttttacg
    12421 ctggctgcaa cgcgaatttg gtcatgcgcc aacggctgcc gtcggctatt cgctcggcgg
    12481 taatatgctg gcctgtttgc tggcaaagga aggcaatgat ctcccggttg atgcggcggt
    12541 gattgtctct gcgccgttta tgctggaagc ctgtagctat catatggaaa agggcttttc
    12601 ccgcgtttat cagcgttact tgctgaacct gttaaaagcc aatgccgcgc gcaagctggc
    12661 agcctacccc ggaacgctgc cgattaatct cgcgcagtta aaatcggtac gtcgcatccg
    12721 tgaatttgac gatctaatca ccgccagaat tcacggctac gccgacgcta tcgactatta
    12781 tcgtcagtgt agcgccatgc caatgctgaa ccggatcgcc aaaccgacgc tgattattca
    12841 cgccaaagac gatccgttta tggatcatca ggtgatccca aaaccggaaa gtctcccctc
    12901 gcaggtggag tatcaactga ctgaacatgg cggtcatgtt ggctttattg gcggtacatt
    12961 acttcatccg caaatgtggc tggagtcacg cattcctgac tggttaacaa cgtatctgga
    13021 ggcgaaatca tgttgattcc gtggcaagac ctctcccccg aaacgctgga aaatttaatt
    13081 gaaagctttg tgttacgtga aggcaccgat tatggtgaac atgagcgtac acttgaacag
    13141 aaagtcgccg acgtcaaacg ccagctacag tgcggagaag cggtgctggt atggtcggag
    13201 ctgcacgaaa cggtcaatat tatgccgcgc agtcagtttc gcgaataacc tgcaaccgtc
    13261 ggcggacgag ataatagtga tagctaagtc atggagttac tatgtctgcc aaacatccgg
    13321 tcattgcggt aacaggatcc agcggcgcgg ggaccaccac caccagcctc gcgtttcgta
    13381 aaatattcgc gcagttaaat ctgcatgcag ctgaggtgga aggcgacagt tttcaccgtt
    13441 acacccgccc ggaaatggac atggcgatcc gcaaagcgcg cgacgccggg cggcatatca
    13501 gctacttcgg ccccgaagcg aacgacttcg gcctgctgga acaaaccttc attgaatacg
    13561 gtcagagcgg caaagggaaa tctcgcaaat atctgcatac ctacgacgaa gccgtaccct
    13621 ggaatcaggt accgggaaca ttcaccccct ggcaaccttt accagaaccc actgatgtac
    13681 tgttttatga aggtttacac ggcggcgtag tcacgccaca gcataacgtt gcgcagcatg
    13741 tggacttact ggtcggcgta gtgcctatcg ttaaccttga gtggattcaa aaactgatcc
    13801 gcgacaccag cgagcgcggg cactcacgag aagcagtgat ggactcggta gtgcgttcaa
    13861 tggaagacta tatcaactac atcacaccgc agttttcccg cacccatctt aacttccagc
    13921 gcgttcccac cgtcgacact tcaaacccgt tcgcggcaaa aggtatcccg tcgctggatg
    13981 aaagctttgt ggtgatccat tttcgtaatc tggaagggat cgatttccct tggctgctgg
    14041 cgatgttgca aggctcattc atttcccaca tcaatacgtt agtggtacct ggcggtaaaa
    14101 tggggctggc aatggaatta attatgctgc cgctggtgca acgattgatg gaaggaaaga
    14161 aaatcgagta actttgctat tatgccggat aaaatactat ccggcttcac aacgggatag
    14221 ttaagtcacg cggcaaccac ttcatacgag tgagtaatat tcaccgcttt ttccagcatc
    14281 aacgccactg aacaatattt ctcggcagag agatcaaccg cacgcgcaac cgctgcgtct
    14341 ttcaggtcgc gaccggtgac gataaaatgc agattaatgt gcgtaaacag gcgtggtgcc
    14401 tcttcgcggc gttcagaggt caattttact tcacaatcga ccacatcctg acgccctttt
    14461 tgcaggatcg aaaccacatc gatggcactg caaccacccg ccgccatcaa caccatttcc
    14521 atcggacttg gtgctttatc gcctgagttg ccgtccatta aaatctggtg accagaggcg
    14581 gattcgccca gaaaagttaa cccttcgacc cacttcactc gcgcttgcat ttttgctact
    14641 ccactgcgtc aattttcctg acagagtacg cgtactaacc aaatcgcgca acggaaggcg
    14701 acctgggtca tgctgaagcg agacaccagg agacacaaag cgaaagctat gctaaaacag
    14761 tcaggatgct acagtaatac attgatgtac tgcatgtatg caaaggacgt cacattaccg
    14821 tgcagtacag ttgatagccc cttcccaggt agcgggaagc atatttcggc aatccagaga
    14881 cagcggcgtt atctggctct ggagaaagct tataacagag gataaccgcg catggtgctt
    14941 ggcaaaccgc aaacagaccc gactctcgaa tggttcttgt ctcattgcca cattcataag
    15001 tacccatcca agagcacgct tattcaccag ggtgaaaaag cggaaacgct gtactacatc
    15061 gttaaaggct ctgtggcagt gctgatcaaa gacgaagagg gtaaagaaat gatcctctcc
    15121 tatctgaatc agggtgattt tattggcgaa ctgggcctgt ttgaagaggg ccaggaacgt
    15181 agcgcatggg tacgtgcgaa aaccgcctgt gaagtggctg aaatttcgta caaaaaattt
    15241 cgccaattga ttcaggtaaa cccggacatt ctgatgcgtc tgtctgcaca gatggcgcgt
    15301 cgtctgcaag tcacttcaga gaaagtgggc aacctggcgt tcctcgacgt gacgggccgc
    15361 attgcacaga ctctgctgaa cctggcaaaa caaccagatg ctatgactca cccggacggt
    15421 atgcaaatca aaattacccg tcaggaaatc ggtcagattg tcggctgttc tcgtgaaacc
    15481 gtgggacgca ttctgaagat gctggaagat cagaacctga tctccgcaca cggtaaaacc
    15541 atcgtcgttt acggcactcg ttaatcccgt cggagtggcg cgttacctgg tagcgcgcca
    15601 ttttgtttcc cccgatgtgg cgcagactga tttatcaccc cgatatcaac tatgcacttc
    15661 gacaaacgct ggtgctatgt ttgcccgtgg ccgttgggtt aatgcttggc gaattacgat
    15721 tcggtctgct cttctccctc gttcctgcct gttgcaatat tgcgggcctt gatacgcctc
    15781 ataaacgttt tttcaaacac ttaatcattg gtgcgtcgct gtttgccacc tgtagcttac
    15841 tgacacagct gctactggta aaagatgttc ccctgccctt tttgctgacc ggattaacgc
    15901 tggtacttgg cgtcactgct gagctggggc cattgcacgc aaaattgctt cctgcatcgc
    15961 tgctcgccgc catttttacc ctcagtctgg cgggatacat gccggtctgg gaaccattgc
    16021 tcatctatgc gttgggcact ctctggtacg gattgtttaa ctggttttgg ttctggatct
    16081 ggcgcgaaca accgctgcgc gagtcactaa gtctgctgta ccgtgaactg gcagattatt
    16141 gtgaagccaa atacagcctg cttacccagc acaccgatcc tgaaaaagcg ctgccaccgc
    16201 tgctggtgcg ccagcaaaaa gcggtcgatt taattactca gtgctatcag caaatgcata
    16261 tgctttccgc gcaaaacaat accgattaca agcggatgct gcgtattttc caggaggcgc
    16321 tggatttgca ggaacatatt tcggtcagtt tgcatcagcc ggaagaggtg caaaaactgg
    16381 tcgagcgtag ccatgcggaa gaagttatcc gctggaatgc gcaaaccgtc gccgctcgcc
    16441 tgcgcgtgct ggctgatgac attctttacc atcgcctgcc aacgcgtttt acgatggaaa
    16501 agcaaattgg cgcactggaa aaaatcgccc gccagcatcc ggataatccg gttgggcaat
    16561 tctgctactg gcatttcagc cgcatcgccc gcgtgctgcg cacccaaaaa ccgctctatg
    16621 cccgtgactt actggccgat aaacagcggc gaatgccgtt gcttccggca ctgaaaagtt
    16681 atctgtcact aaagtctccg gcgctacgca atgccggacg actcagtgtg atgttaagcg
    16741 ttgccagcct gatgggcacc gtgctgcatc tgccgaagtc gtactggatc ctgatgacgg
    16801 tattgctggt gacacaaaat ggctatggcg caacccgtct gaggattgtg aatcgctccg
    16861 tgggaaccgt ggtcgggtta atcattgcgg gcgtagcgct gcactttaaa attcccgaaa
    16921 gttacaccct gacgttgatg ctgattacca ccctcgccag ctacctgata ttgcgcaaaa
    16981 actacggctg ggcaacggtc ggttttacta ttaccgcagt gtataccctg caactgttgt
    17041 ggctgaacgg tgagcagtac atccttccac gtcttatcga taccattatt ggttgtttga
    17101 ttgctttcgg tggtaccgtc tggctgtggc cgcagtggca gagcgggtta ttgcgtaaaa
    17161 acgcccatga tgctttagaa gcctatcagg aagcgattcg cttgattctg agcgaggacc
    17221 cgcaaccaac gccactggcc tggcagagaa tgcgggtaaa tcaggcgcat aacactctgt
    17281 ataactcatt gaatcaggcg atgcaggaac cggcattcaa cagccattat ctggcagata
    17341 tgaaactgtg ggtaacgcac agccagttta ttgttgagca tattaatgcc atgaccacgc
    17401 tggcgcggga acaccgggca ttgccacctg aactggcaca agagtattta cagtcttgtg
    17461 aaatcgccat tcagcgttgt cagcagcgac tggagtatga cgaaccgggt agttctggcg
    17521 atgccaatat catggatgcg ccggagatgc agccgcacga aggcgcggca ggtacgctgg
    17581 agcagcattt acagcgggtt attggccatc tgaacaccat gcacaccatt tcgtcgatgg
    17641 catggcgtca gcgaccgcat cacgggattt ggctgagtcg caagttgcgg gattcgaagg
    17701 cgtaatacag gctaaaccat cggataaggc attcacgccg catccgacat attttgcctg
    17761 atacgtctta tttacgcccc aaccaccttc gccaccgcgt gggcgaaacg ttgcatccct
    17821 tcatcgatat ccgcatcttc caccaccagc gacggtgcta aacgcatcac atccggtccg
    17881 gcattcagca ccattacgcc aacctctgca cccgcataca ggaaatcacg cgcccgacct
    17941 ttgtagtgtg gtttcagctc cgcgccaatt aacagcccca taccgcgaat atcgctaaac
    18001 acatcgtact gctgatcgat cttctgcaaa tggtcaacaa aacgctggcg tttcgcctga
    18061 atgccttcca gcacttcagg ggtattgatg atatcaaacg ccgcgcccgc tactgcacag
    18121 gccagaggat taccgccgta ggtggaaccg tgagaaccag gatgaaacgc agaagcaatt
    18181 tccgccgtgg tcagcatggc gctaatcggg aagccgccgc ctaacgcttt cgcagaagtc
    18241 agaatatccg gcgtcacacc gtagtacctg taagcaaaca aatcgccagt ccgccccatc
    18301 ccgcactgca cttcatcaaa caccaataat gcctgatgtt gatcgcacag ctcgcgcaag
    18361 ccctgcaaaa actctggcgt cgctgccgtc acaccgccct cgccctggat cggctcaacc
    18421 accaccgcac aggtgtgatc atccatcacc gctttcactg catggagatc gttaaaggga
    18481 acgtggatga tgtctgccgg tttcggccca aagccgtcgg aatattttgg ctgcccaccc
    18541 accgaaacgg taaacagcga gcgaccatga aaagcgttat ggaaggcaat aattttggtt
    18601 ttgaacgggc tatgacgcac acaggcgtaa tggcgtgcca gtttaaaggc ggtttcgtta
    18661 gcttccgtgc cggagttcat aaacaccacg cgttcggcaa acgtcgcctc aatcagtttg
    18721 cgcccaagac gcagcgccgg ttcattggtg aaaacgttac tgatatgcca cagagtttcg
    18781 ccctgggttt ttaacgcgtt caccagcgca ggatggcaat ggcccaacgc cgtaactgca
    18841 atgccacccg cgaaatcgac atactccttg ccttgctgat cccagattcg gctgccctga
    18901 ccttttaccg gaataaactc tgccggagca taaatcggca ggatcacttc atcgaaagtc
    18961 gcgcgtgtaa ttgctgtttg ttcaattgcc atctcatgat caccctgtta cgcataaata
    19021 aatgtgaaat tataatcaca aaatatgcat aaaaaatcac tgaatggcaa tcggaaatca
    19081 gagatgcagg aaattagcca gcagttgatg tccttgttcg ctaagaatac tttctggatg
    19141 gaactgcaca ccttccagat cccactggcg atggcgaatc cccataatct ctcgggtttc
    19201 gctccaggcc gtcacgtcaa agcacgctgg taatgagtca ggttccacca ccagcgaatg
    19261 gtagcgtgtc acggtaagtg gatttgccag cccccgaaat acgccctcac cgctatgtgt
    19321 aatcggcgag gttttgccgt gcatgacctt tgcggcgcgc acaactttgc cgccaaatgc
    19381 ctgcgccatt gcctgatgac cgaggcagac gccaagaatc ggcaagcgcc cggcatagtg
    19441 gcgaataacg tcaagggaga tcccggcttc atctggcgta caggggccag gtgagatgac
    19501 aattttttgt ggtttaaggg cgtcgatatc cgccagcgtc aacgcatcgt tgcgcttaac
    19561 cagcacatcc gcccccagtt cacaaaagta ctggtagagg ttccaggtaa aagaatcgta
    19621 gttatctata agcaggatca tggcggctcc ggtacgaaag aaccgcgcta ttttactcag
    19681 attctccggc ttcgcttacc actttgctaa atatcatctg cagtgcggtg agatccccca
    19741 ttgccccact ctgatttgcc tgattccagg cctctttttc gataccctgc cagctcagtt
    19801 gatatccggc atgaatcgcc agttgctcga agaagatccg ctgtgccaga ccacttccca
    19861 cccggaaggg atgcagcacg ttgatttcac aatagtaatg cgccagccgc tcgacgaact
    19921 tcgctttctc caggccaacc agataacctt cttcctccag atcctgcatc agggcattgc
    19981 cctctttttc gatataagca aagtggcaga acggcgtatc gccctgataa atatcaactt
    20041 cacgcagttg ccctgcccag tcgaaaatat cctggtacag ctggcgatgg atagtgcgca
    20101 aatgcggtaa accgcgcacc agcggaccaa gctcaatggt cgcagcacgc agcgccgtca
    20161 tttcgtaagc ggcctgttcc agccgctgct gctggcggat gttcagccgg ttacgcatga
    20221 tatcaaggcc aggataaaga tacggatcgc gcccttcgcc gaatttatcg ctcatagtgc
    20281 ctcctcagct cttcaaggcg cgctaaagcc tctgcggcag taagagtgac taaaggcatc
    20341 tcgacgcctt caagacggcg actggcctgg aaattacgat tacgctgaag ctcccagaga
    20401 cgggactttt gcttatcggt gagtttcttc acttgatgcc tccctgaacg tgtccatttg
    20461 ccacaagtat aagcggcaaa tccgggttac gccgggagag cagaagcgcg ggcaagattg
    20521 cccgcgcgag aaatcattac ggcaggactt tagcggaaag gataactacc ggttttgacg
    20581 gcacattctg gtacggacca acatcatgag tcggcacctg ggaaatctta tcggcaacgt
    20641 ccatgccttt caccacttta ccaaataccg cgtaaccgaa atcacgctga ccatggtcaa
    20701 ggaaggcgtt atcggcaacg ttgataaaga actggctggt ggcgctgtct ttgtcagcgg
    20761 tacgcgccat cgcgatggtg ccacgcgtgt tgcgcaggcc gttatcggct tcatttttga
    20821 ttggcgggtt tggttttttc tgctgcatct gctcggtgaa accgccgccc tgaatcataa
    20881 agccaggaat gacgcggtga aaggtagtgt tgttataaaa accgctgttc acataatcga
    20941 caaagttttg cacagacact ggcgcttttt gtttatccag ctccagttcg atgttaccag
    21001 ctgaggttgt caacaatacg tgcgggtccc ctttcgctgc cattgctgcg ggagaaagag
    21061 cagaaagagc agaaagagcg aaaacagcag ccatcgccgc cagggtcgat ttgaacatga
    21121 gatttcctta acaaacagag cagaaaaaaa gcaagtgaag agattctaaa tagccttcaa
    21181 gaacaaggcc atccctttac ctaattttac gtatctgaaa tatatgtaac ctgacgatca
    21241 ctttttattc cgtgatcaaa atcacctctt aaaatgcaat ttagcaaccg attgcaataa
    21301 aacatttaaa cagatcataa aatcacctaa aatcgcccgc tcgcggcata tcccgctatg
    21361 ccattttatt tttgaacgca ggccaatcat gactaacagc aatcgcatca agctcacatg
    21421 gattagcttt ctctcctacg cactgactgg tgcgttggtt attgtcaccg ggatggtgat
    21481 gggaaatatc gccgattatt tcaatctgcc tgtttccagt atgagtaaca ccttcacctt
    21541 cctcaacgcc ggcattttaa tctctatctt cctcaacgcc tggctgatgg aaatcgtccc
    21601 gttgaaaacg cagttacgtt ttggctttct cctgatggtg ctggcggttg ccggtttgat
    21661 gttcagccac agcctggcgc tgttctcgac ggcgatgttc attctcgggg tggtcagcgg
    21721 cattactatg tcgattggta cattcctgat aacacaaatg tatgaagggc gtcagcgcgg
    21781 ttcccgcctg ttatttactg actccttctt cagtatggct gggatgattt tcccaatgat
    21841 cgccgcgttt ctactggcgc gcagcattga gtggtactgg gtttatgcct gcatcgggct
    21901 ggtgtatgtc gctattttta ttctgacctt cggctgtgag ttcccggcac tgggcaaacg
    21961 tgcaccaaaa acggatgctc cggtagagaa agaaaagtgg gggatcggcg tactgtttct
    22021 ctctgttgcg gcgctgtgct acatcctcgg tcagttaggt tttatctcct gggtgcctga
    22081 gtatgccaaa ggcctgggca tgagcctgaa cgacgcgggc acgctggtga gtaacttctg
    22141 gatgtcatac atggtcggca tgtgggcgtt cagctttatt cttcgcttct ttgatttgca
    22201 acgcattctg accgtactgg ctggtctggc tgcgattctg atgtacgtct ttaacaccgg
    22261 aacaccagta catatggcgt ggtcaattct cgctctgggc ttcttctcca gcgcgatcta
    22321 taccaccatc atcaccctgg gttcacagca gaccaaagta ccgtcgccaa aactggttaa
    22381 ctttgtcctg acctgcggaa ccatcggtac tatgttgacc tttgtggtta ccggcccgat
    22441 tgttgaacat agcggtccgc aggcggcact gcttacagca aacggtctgt acgctgtcgt
    22501 ctttgtgatg tgcttcctgt taggtttcgt cagccgtcac cgtcagcata acaccctgac
    22561 ctctcattaa ttgctaatgc cggacggcac tatcgtcgtc cggccttttc ctctcttccc
    22621 ccgctacgtg catctatttc tataaacccg ttcattttgt ccattttttg cacaaacatg
    22681 aaatgtcaga caattccgtg acttaagaaa atttatacaa atcagcaata taccccttaa
    22741 ggagtatata aaggtgaatt tgatttacat caataagcgg ggttgctgaa tcgttaaggt
    22801 aggcggtaat agaaaagaaa tcgaggcaaa aatgagcaaa gtcagactcg caattatcgg
    22861 taacggtatg gtcggccatc gctttatcga agatcttctt gataaatctg atgcggccaa
    22921 ctttgatatt accgttttct gtgaagaacc gcgcatcgct tatgaccgcg tacacctctc
    22981 gtcttacttc tctcaccaca ccgccgaaga gctgtcgctg gtgcgcgaag gcttctacga
    23041 gaaacacggc atcaaagttc tggtcggcga acgcgctatc accatcaacc gtcaggagaa
    23101 ggtgattcac tccagcgccg gacgtaccgt tttttatgac aagctgatca tggcaaccgg
    23161 ttcctacccg tggatcccac caatcaaagg ttctgatact caggactgct ttgtctatcg
    23221 caccattgaa gacctcaacg ccattgaatc ctgcgcccgt cgcagcaaac gcggtgccgt
    23281 tgttggtggc ggcctgttag gtctggaagc cgctggcgcg ctgaaaaact taggtattga
    23341 aacccacgtt atcgaatttg cccctatgct gatggcggaa cagcttgatc agatgggtgg
    23401 cgagcagctg cgtcgcaaaa tcgaaagtat gggcgtgcgc gttcacacca gcaaaaacac
    23461 ccttgagatt gtgcaggaag gtgtcgaagc gcgtaaaacc atgcgttttg ccgacggcag
    23521 cgaactggaa gtcgacttta tcgtcttctc taccggtatc cgtccgcgcg ataagctggc
    23581 aacccagtgt ggtctggacg ttgctccgcg tggggggatt gtcattaatg attcctgcca
    23641 gacttccgat ccggatatct acgccatcgg tgaatgcgca agctggaaca accgtgtatt
    23701 tggtctggta gcaccaggct acaaaatggc gcaggtcgcc gttgaccata ttctgggtag
    23761 cgaaaacgcc tttgaaggtg ctgaccttag cgccaagctg aaactgctgg gcgtagacgt
    23821 aggtggtatt ggtgatgcgc acggtcgcac gcctggcgca cgtagctatg tttacctcga
    23881 cgaaagtaaa gagatctaca aacgcctgat tgtcagcgaa gacaacaaaa ccctgctcgg
    23941 tgcggtactg gtgggcgata ccagcgacta cggcaacctg ctgcaactgg tgctgaacgc
    24001 tatcgagctg ccggaaaacc cggattctct gattctgccg gcgcactcgg gtagcggtaa
    24061 gccgtctatc ggtgttgata aactgccgga cagcgcgcaa atctgctcct gcttcgacgt
    24121 cactaaaggt gatctgattg ctgccatcaa caaaggctgc cacacagttg cggcgctgaa
    24181 agctgaaacc aaagcgggta ctggctgcgg tggctgtatc ccgctggtca ctcaggtact
    24241 gaacgcggaa ctggcgaaac agggcatcga agttaacaac aacctgtgcg aacactttgc
    24301 ttattcgcgt caggaactgt tccatttgat ccgcgttgaa ggcattaaaa ccttcgaaga
    24361 actgctggcg aaacacggca aaggctacgg ttgtgaagtt tgtaaaccaa ccgtcggttc
    24421 gctgctggcc tcctgctgga acgaatacat tctgaagccg gaacatactc cgctgcagga
    24481 ttctaacgac aacttcctcg ctaacatcca gaaagacggc acctactcgg tgatcccgcg
    24541 ttctccgggc ggtgaaatca ccccggaagg gctgatggca gtgggtcgta tcgcgcgtga
    24601 atttaatctc tacaccaaaa tcaccggctc ccagcgcctg gcgatgtttg gcgcacagaa
    24661 agacgatctg ccggaaatct ggcgtcagct gattgaagct ggcttcgaaa ccggtcatgc
    24721 ctatgcgaaa gcactgcgta tggcgaaaac ctgcgtgggt agcacctggt gccgctacgg
    24781 tgttggcgat agcgtcggcc tcggcgtgga actggaaaac cgctacaaag gcatccgtac
    24841 gccgcacaaa atgaagttcg gtgtctccgg ctgtacccgt gaatgttcag aagctcaggg
    24901 taaagacgtg ggtattatcg ccactgaaaa aggctggaac ctgtatgttt gcggtaacgg
    24961 cggcatgaaa ccgcgtcatg cggatctgct ggcagcggat atcgatcgcg aaacgctgat
    25021 caaatatctc gaccgcttca tgatgttcta catccgtacc gccgacaaac tgacgcgtac
    25081 cgcgccgtgg ttagaaaacc tcgaaggcgg catcgattac ctgaaagcgg tgatcattga
    25141 cgacaaactg gggctgaacg ctcatctgga agaagagatg gcgcgcctgc gtgaagcggt
    25201 agtgtgtgag tggactgaaa cggtcaatac accgtccgcg cagactcgct tcaaacactt
    25261 catcaacagc gacaagcgtg acccgaacgt gcagatggtg ccagagcgcg aacagcaccg
    25321 tccggcaacg ccgtatgaac gtatcccggt aactctggtg gaggacaacg catgagccag
    25381 tggaaagaca tctgcaaaat cgatgacatc ctgcctgaaa ccggcgtctg cgcactgtta
    25441 ggtgacaagc aggtagcgat tttccgcccg tatcacagcg accaggtgtt tgcgatcagc
    25501 aacattgatc cgttcttcga gtccagcgtg ctatcacgcg ggctgattgc ggaacaccag
    25561 ggcgagctgt gggtcgccag cccgctgaaa aaacagcgtt ttcgcttaag tgatggcttg
    25621 tgcatggaag acgaacagtt ttccgtcaaa cattacgaag cacgagtgaa agacggcgtg
    25681 gtgcagctgc gcggttaatg ttttaacggg aggcgcaatg cctccccttt ttgcatggtc
    25741 ctgtaatgac cttcggtata ttgcaggaca ttttttaaac tttttgtttt attttttgtt
    25801 tttatttttt aaaggataat caaatgttta cagacactat taataagtgt gcggctaatg
    25861 ctgcgcgcat tgcacgcctg tcggcaaata acccgctcgg cttttgggtc agctccgcca
    25921 tggcgggcgc atatgtgggt cttgggatca tcctgatttt cacgctcggt aatttgctcg
    25981 acccgtccgt acgtccgctg gtgatgggcg cgacctttgg tatcgcctta acgctggtga
    26041 ttatcgccgg ttctgaactg ttcaccggac acaccatgtt cctcaccttt ggggtaaaag
    26101 cgggcagcat cagccacggg caaatgtggg caatcctgcc gcaaacctgg ctgggtaacc
    26161 tggtcggttc cgtcttcgtt gccatgctct atagctgggg cggcggtagc ctgctgccgg
    26221 tagataccag catcgttcac tccgtcgcgc tggctaaaac cactgcaccg gcaatggtac
    26281 tgttcttcaa aggtgcattg tgtaactggc tggtttgcct agcaatctgg atggcgctgc
    26341 gcactgaagg ggcggcgaaa tttatcgcta tctggtggtg tctgctggca tttatcgcgt
    26401 ccggctacga gcactctatc gctaacatga cgctgttcgc gctctcctgg ttcggcaacc
    26461 acagcgaagc ctacacgctg gcgggtattg gtcataacct gctgtgggtg acgctgggta
    26521 atactttatc aggtgccgta ttcatgggat tgggttattg gtatgctacg ccgaaagcga
    26581 atcgtccggt tgcggacaaa tttaatcaaa ctgaaacagc tgccggttaa ttactaaggg
    26641 gtttttacgt ggatcatttg cctatatttt gccaattacg cgatcgcgac tgtctgattg
    26701 tcggcggtgg tgatgtcgcg gaacgcaaag caaggttgct gttagacgca ggcgctcgct
    26761 taacggtgaa tgcattagcg tttattccac agttcaccgc atgggcagat gcaggcatgt
    26821 taaccctcgt cgaagggcca tttgatgaaa gccttctcga cacctgctgg ctggcgattg
    26881 cagcgacgga tgatgacacg cttaaccagc gcgtcagcga agccgctgaa gctcgtcgca
    26941 tcttctgtaa cgtagttgat gcgccgaaag ccgccagctt tattatgccg tcgattattg
    27001 accgctcacc gctaatggta gcggtctcct ctggcggcac ctctccggtt ctggcgcgcc
    27061 tgttgcgcga aaaactcgaa tcactgctgc cgttgcatct gggccaggtg gcgaaatatg
    27121 ccgggcaatt acgcgggcga gtgaaacaac agttcgccac gatgagtgag cgtcgccgtt
    27181 tctgggagaa attgttcgtt aacgaccgcc tggcgcagtc gctggcaaac aacgatcaga
    27241 aagccattac tgaaacgacc gaacagttaa tcaacgaacc gctcgaccat cgcggtgaag
    27301 tggtgctggt tggtgcaggt ccgggcgatg ccgggctgct gacgctgaaa ggactgcaac
    27361 aaattcagca ggcagatgtg gtggtctacg accgtctggt ttctgacgat attatgaatc
    27421 tggtacgccg cgatgctgat cgcgttttcg tcggcaaacg cgcgggatac cactgtgtac
    27481 cgcaggaaga gattaaccag atcctgctgc gggaagcgca aaaaggcaaa cgcgtggtgc
    27541 ggctgaaagg tggcgatccg tttatttttg gccgtggtgg cgaagagctg gaaacactgt
    27601 gcaacgcggg tattccgttc tcggtggttc cgggtattac cgcagcttct ggttgctctg
    27661 cctattcggg tattccactc acgcatcgcg attatgccca gagcgtacgc ttaattaccg
    27721 gacacttaaa aaccggtggc gagctggact gggaaaacct ggcggcagaa aaacagacgc
    27781 tggtgttcta tatggggttg aatcaggccg cgactattca gcaaaagctg attgaacacg
    27841 gtatgcctgg cgaaatgccg gtggcaattg tcgaaaacgg aacggcagtc acgcagcgcg
    27901 tgattgacgg tacgctcacg caactcggtg aacttgctca gcaaatgaac agtccatcgc
    27961 taattattat tggtcgggtt gttggcctgc gcgataaact gaactggttc tccaatcatt
    28021 aatttaaccc gggccagaga attctggcct tcttaatgac tctttttata tattcagcaa
    28081 ataaaacata accccttaat aaatattatc tgctaatggg ttatattcac cgttacagcg
    28141 acttacaaaa ttaagtcatt cgctcactga cgccaattaa atattcctgt atcgttctta
    28201 acaagcagtc tgcttatatt catataatca atgaatatta attaatatat aatacatagg
    28261 gaatgtaaat gaacaaattt attaaagttg cactggtagg tgcagtactg gctacgttaa
    28321 ctgcatgtac tggtcatatt gaaaaccgtg ataagaactg ctcttacgac tacctgctgc
    28381 acccggcaat ttctatttct aaaatcattg gcggttgcgg tcctactgca cagtaagtct
    28441 ctgacagaaa tcggctaaca ccggtttaat tgcccgacgc cctctggatt ttccgagggc
    28501 gtatttgttt ctatgtcctt cttgcccccg cccgtaaaaa tattttcact ttaaattcaa
    28561 ttttataact acatccatta gccacagcgc gatcctgtgc gaaattttgt gatcttcctc
    28621 cacattacat aacataatat gttgttatat tcatcatgca ttgtcatgtt accttttaaa
    28681 tgactgcaaa ctctccccta caacgtattg gacaagaaaa aggtatcgct atgggaagcc
    28741 aagaactcca acgcaagctc ggattttggg ccgttcttgc aatcgccgtc gggacaaccg
    28801 tcggctccgg tatttttgta tctgtgggtg aagtggcaaa agcagcgggc acgccgtggc
    28861 ttacggtgct cgcgtttgtc attggcgggt taattgtgat cccgcaaatg tgcgtctatg
    28921 cggaactatc caccgcttat ccggaaaatg gcgcagatta tgtttatctg aaaaatgccg
    28981 gaagccgacc gctggctttc ctctccggct gggccagctt ctgggccaac gatgcgccgt
    29041 cattgtcgat tatggcgctg gcgattgtca gcaatcttgg ctttttaacg cctatcgatc
    29101 cgttgctcgg taaatttatc gccgccggat taattatcgc ctttatgttg ctacacctgc
    29161 gctccgttga aggcggcgca gcgtttcaga cgctaattac catcgccaaa attatcccgt
    29221 tcactatcgt cattggcctt gggatcttct ggtttaaagc ggagaatttt gccgccctta
    29281 ccaccactgc gattggcgca acgggcagct ttatggcgct gctggcgggg atctctgcca
    29341 ccagttggtc gtataccggc atggcctcta tctgttatat gaccggcgaa attaaaaacc
    29401 ccggaaaaac catgccacga gcgctgattg gttcctgtct gctggttctg gtgctctaca
    29461 ccctgctggc gctggtgatt tccggcctga tgcccttcga caaactcgcc aattctgaaa
    29521 cgccgatttc cgacgccctg acctagatcc ccgcactcgg cagcaccgct gggctctttg
    29581 ttgccatcac ggcgatgatc gtcattcttg gttcgctttc cagctgcgtg atgtaccagc
    29641 cgcggctgga atacgcgatg gcgaaagaca acctgttctt taaatgcttc ggccatgtgc
    29701 atccgaaata caacacgccg gatgtctcca tcatcctgca aggggcgctg gggatcttct
    29761 tcatcttcgt ttccgatctc accagcctgc tgggttattt caccctggtg atgtgtttca
    29821 aaaataccct caccttcggc tccatcatct ggtgtcgtaa acgcgacgat tacaaaccgc
    29881 tgtggcgtac tccggctttc gggctgatga ccaccctcgc cattgcgtca agcctcattc
    29941 tggtcgcctc aacctttgtc tgggcaccga ttcccggcct tatctgcgcc gtcatcgtta
    30001 ttgctactgg tctgcctgct tacgccttct gggcgaagcg tagccgccag ctcaacgctt
    30061 tgtcgtaatg ttatctgaag aaaataaaat gttggatatt gataaaagca ccgtggactt
    30121 tctggtcacc gaaaatatgg ttcaggaagt ggaaaaagtt ctcagccatg acgttccgct
    30181 ggtgcacgcc atcgtggaag agatggtgaa gcgcgacatt gatcgtattt atttcgttgc
    30241 ctgcggctcg ccactcaacg cggcgcaaac ggcgaaacat ctggcggatc gcttttccga
    30301 tcttcaggtc tacgccattt ccggctggga gttctgcgat aacaccccgt atcgcctcga
    30361 cgatcgttgc gcggtgattg gcgtttctga ctacggtaaa acggaagagg taatcaaagc
    30421 gctggagctg ggccgggcct gcggcgcact cactgcggcg ttcaccaaac gcgcggacag
    30481 cccgattacc tcggcggcgg aatttagcat tgattatcag gccgactgta tctgggaaat
    30541 tcacctgctg ctctgctaca gcgtggtgct ggagatgatc acccgcctcg cgccgaacgc
    30601 ggaaatcggc aagatcaaaa acgatctcaa gcagttgccg aatgcgctcg gtcatctggt
    30661 acgcacctgg gaagaaaaag gccgccagct tggtgaactg gccagccagt ggccgatgat
    30721 ttataccgtt gctgcgggtc cgctgcgtcc gctgggttac aaagaaggca ttgtaacgct
    30781 gatggaattt acctggacgc acggctgcgt gattgagagc ggagagttcc gccatggccc
    30841 gctggagatt gtcgaaccgg gcgttccgtt cctgttcctg ctcggcaatg atgaaagtcg
    30901 ccacaccacc gaacgcgcca ttaactttgt taaacagcgt actgacaacg tgatcgtcat
    30961 cgattacgcc gaaatttcgc aagggctgca cccgtggctg gcaccgttcc tgatgttcgt
    31021 gccaatggag tggctctgct actacctgtc tatttacaaa gatcacaacc cggatgaacg
    31081 ccgctattac ggtggtctgg tggaatatta atccctctcc cggcccggca acgggccgga
    31141 tttacgcaag gagttacccg atgaaaacag gtatgtttac ctgcggccac cagcggctgc
    31201 ctattgaaca cgcatttcgt gatgcaagcg agctgggtta cgacggcatc gaaatttggg
    31261 gcggtcgccc gcacgcgttc gcgccggact taaaagcggg cggcatcaaa caaatcaagg
    31321 cgctggcgca gacgtatcag atgccgatta tcggttacac gccagaaacc aacggctatc
    31381 cgtataacat gatgctgggc gatgaacata tgcgtcgtga aagcctggac atgatcaagc
    31441 tggcgataga tatggcaaaa gagatgaacg cgggttatac gctgatttct gcggcccacg
    31501 cgggctatct cacgccgcct aacgttatct gggggcggct ggcagagaac ttaagcgaac
    31561 tgtgtgagta cgcggaaaac atcggcatgg atctgatcct cgaaccgtta acgccgtatg
    31621 aatcgaacgt cgtgtgtaat gctaatgatg tgcttcatgc gctggcgctg gtgccttcgc
    31681 cgcgcctgtt cagcatggtc gacatttgcg cgccgtatgt ccaggcggaa ccggtgatga
    31741 gttatttcga caaactgggc gataaattac gtcatctgca tattgtcgac agcgacgggg
    31801 ccagcgacac gcattacatt cctggagaag gcaaaatgcc gctgcgggaa ctgatgcgcg
    31861 atattattga tcggggctat gagggttact gtacggtgga gctggtgacg atgtatatga
    31921 acgagcccag actctatgcc cgccaggcgc tggaacgctt tcgcgcgctg ctgccggagg
    31981 atgagagatg aaaaccctgg cgacaatcgg cgataactgc gtcgatatct acccgcaact
    32041 gaataaagcg ttttctggcg gtaatgcggt caatgtggcg gtgtactgca ctcgctacgg
    32101 catacagccg ggatgcatta cctgggtggg tgacgatgac tacggcacaa agctgaagca
    32161 ggatctcgcc cgcatgggcg tcgatatcag ccatgtccat acgaaacacg gcgttaccgc
    32221 acaaactcag gtggaactgc acgacaatga tcgcgttttt ggcgactaca ccgaaggcgt
    32281 gatggccgac tttgccctga gtgaagagga ttacgcctgg ctggcgcagt atgacattgt
    32341 gcacgcggca atctggggac atgcggaaga cgcattccca cagctgcacg ctgcgggcaa
    32401 acttaccgct ttcgacttct ccgacaagtg ggacagcccg ctctggcaga cactggtgcc
    32461 gcatctcgat tttgcctttg cctccgcacc gcaagaagac gaagcgctgc gtctgaagat
    32521 gaaagcgatt gttgcccgtg gcgcaggaac agtgattgtc acgctgggtg aaaacggcag
    32581 cattgcctgg gatggcgcgc agttctggcg tcaggctcct gaaccggtga cggttatcga
    32641 caccatgggt gccggagatt cgttcattgc cggattcctt tgcggctggt ctgcggggat
    32701 gacattaccg caggcgatgg cgcagggaac ggcgtgcgcg gcgaaaacca ttcagtacca
    32761 cggtgcctgg taggtataac gttggcgtga gcatcttcac gccaacgtgc tgttacttgc
    32821 cggaaaacga ccctataatc cgagtaattc attctttatt tcagggtcga ttatgtcagc
    32881 tacggaccgc tactctcatc aactcctcta cgctaccgtc cgccagcggc tgctggatga
    32941 tatcgcgcag ggggtttacc aggccgggca acagatccct accgaaaacg agctttgtac
    33001 acaatataac gtcagccgca ttaccattcg caaagccatc agcgacttag tggcagacgg
    33061 cgtactgatc cgctggcagg gaaaaggcac ctttgtacaa agccagaaag ttgaaaacgc
    33121 cctgcttact gtcagtggtt ttaccgattt tggcgtctca caaggcaagt cgacgaaaga
    33181 gaaagtgatc gaacaggaac gggtcagcgc cgcgccgttt tgcgaaaagc tgaacatccc
    33241 cggaaacagc gaagtgttcc atctctgccg ggtgatgtat ctcgataaag agccgctgtt
    33301 tattgatagt tcatggatcc cgctgtcgcg ttatcctgac tttgatgaga tttacgtcga
    33361 aggaagctcc acctatcagt tatttcagga gcgttttgac acgcgagtgg tcagcgacaa
    33421 aaagaccatc gatatctttg ccgccacccg cccgcaggca aaatggctga aatgcgaact
    33481 gggcgaaccg ttgtttcgca tcagcaaaat cgcctttgat cagaatgaca aaccggtgca
    33541 cgtctccgaa ctcttctgcc gcgccaatcg catcacctta acgattgata ataaaagaca
    33601 ttaaccgtag gccggataag atgcgccagc atcgcatccg gcgatgctgg cgcactgaat
    33661 tttacatccc gcacgttccc ctcaccctaa ccctctcccc aaaggggcga ggggaccgtt
    33721 cagtgcggaa tttagcttga ttgccgaacg gtgctatcca gcattaaata ctcgcaatac
    33781 tctcacgcaa aatccgcagc accgtctctt caccgctgcg attcgggtta atgcgaatcg
    33841 cacaatgttc gagttgtgga ttcgcctggc gaaacgttcc tgaaagtcga tagaagagcg
    33901 gcgggatttc atatttcgac tctgcaccga ccgggtaagg caacgcgcca cgcttttgcg
    33961 cttcttccag caccctggcg gcaatcagct gatgaaactc gacaatcaac accttcgact
    34021 gcgcattggc aatgacggcg cttttcactt ccgccaccgc gccgccgtta agcaaagcca
    34081 gcaaccgttc ggatactccc gcctgcaccg catgcatcac tggcgcgaac accagaccac
    34141 gcaagacttc cagcgcctgt gagccctgga tctggctacc gccggagtaa agcgtggcgc
    34201 gaatccgact aataacatcg gcatcgccaa ccactgcacc aacgcccccc ggcccaaaca
    34261 gcttgaagca ggagaatgtc gagacattcg cgccgcattc acagccgatt cgcgctacct
    34321 tcatcaccgc atagttatca tcggttaacg ctggaacacc tgccgcgcgc aacgttgcca
    34381 gcacatctgc cagcacatag ctgtcctgcg gctgctggcg cgtatgctgc accagcgcgg
    34441 catcaggttg ttgctcgtcg acgacctgct tcagtgccga caggtcattg aaatcagcag
    34501 taataagcgt cagccccatc tgctcaataa taacccgtgt cgtcgggtaa acaggcgcgt
    34561 catgcaccag aagacgctgc cccggtttga gcaaagccgc cagcccggcg cgaatcgcgc
    34621 cagtccccgc gccctgcacc agcgccgcgg cctgtgcgtg aaatgcatca gccagcacct
    34681 gctccacccg ttgggtaata cgcggttgat tcagacttgg agttaaccct aaatcaccgc
    34741 cagcaagaaa ctcgctgcct ggaaaatggc ggcaaatagt gtccaccagc gcaaactgct
    34801 tttgctgcgc ttcagcaaga gtcaggcttt gcaaaggaaa cgtcttcata ggtgccttcc
    34861 ttaagccgga acgaacaggc caagccagta aagaacattc agcagaatac cggtgatcat
    34921 tactgccacg accggtgccg ccattttctg taccggacgg cccagggatt cgttgaggaa
    34981 gtaaatggca accgcgatag agaatccggt ataacccgcc atcttaattg ctgcgaaaat
    35041 cgaaccgacc agcagcgcca cttccatcag catattcatg gcgttacgga tgttatccga
    35101 cgcattacgc accgacgggt agcgtcccag ccatttgccg atcgaacgaa gcagcaagac
    35161 ttccgccgaa ataaccactg cgcctaatac cgctgcaacc atcggattcg gcgagagata
    35221 gcccaccgca taaacaaagg taaagcccgc aactgcatac acacccgttg ctaaggcggt
    35281 ggtggcaatc aacggcacaa accccagtcc gcgcataaat tctgccagag ccgcctgatt
    35341 aatcagcgtt tgcgattgtt ccggcgtcac gcctgcggaa tacgctttct ccagtgtgaa
    35401 gatcgaaact tcgctaccag caaaaatctt catactggca acggcggcaa tcaatgctcc
    35461 cacgatggcg atataaggta agtttttgat aatccgcgac gtgcgctctt caaacaccga
    35521 aagcccgctg gcatcgatgt cattttcatc acgatgacgc aggtcgtgag ttatcgcgat
    35581 ccccagcagc atcaccatgc caataaagat ttcgatggat tcagggttaa gatgtgggaa
    35641 atagcgcacg actaccacac gggtcatcag aacaaccacg gcggcgacca gactttgctt
    35701 ccagccaaac tggtagaaaa tcgccaccag cgggaacagt gcaaaagcag aaaccaccgg
    35761 cgagcttaat tccccgaggc tacctaatac atccaccggc agcgcggtca gcagctggtt
    35821 aactggcaac aggcaagtaa ggatcaacac gccccagata gcgccaagac caaacgccat
    35881 caggctgttt atcgccagta cgccgaggat atcggtagga agaaagagta accatgcgtt
    35941 gagcagcccg gttttcaggg taaaagagat ccccaccgag gccacaaaac caatgctcag
    36001 accaaaagcg atactccccg cctcgcgacg gttcatatag ccttcaatca gttgcggcag
    36061 gatcgggtgg atgccgtcat gaaaaacagc cgccgagcga tgcgccagaa gcgatgtcat
    36121 acccgtcagg cacgccacca cgataatctg aatatacaga tccatagtcg tgccttttat
    36181 ttcagatggt taatcaacat cggaatcgcg tgttcgacgt gctcaacgga aaggccaaac
    36241 gccactttcc cttcagcgat cattttggcg atatgctcgt ctttcgcttt aatgcctggt
    36301 ttggcaatgg tgcaactttt gttatagcca atcaccgcga tggcaatcga caatgccgcg
    36361 cccgcaccgg tattacacgc gccaatgtaa taatctagtt gcccggattt caccttcatt
    36421 gccgcttcca tgtcgttgtg aatgaaaact tcaaaacagc caggagccgt cgcttcaatg
    36481 gtttttttaa tctgctcacg ctgtaagcct gcaacgccaa tctttttcat gagtctgtcc
    36541 ttattggaaa aattgagagg gattttcacg taacatcaca tccacatcgg cctgactgaa
    36601 tcctgactgg cgcaattgcg gaataaaggt ggtcaataaa tagtcatagc cataaccacc
    36661 attggctttt aaatgggagc ggcgcgtaat atccatcgac agcatgacgc ggttcagcaa
    36721 cccacggtca cgtagcgcgt ggagcatcgc aatgcgcttt tcgtccgggt agtaactgtt
    36781 cttaccaatg gtgtcgaact gcacgtacgc gccgagatcg atcatcttca aaatgttgtc
    36841 gaggttgtct ttcagatcgc agtgaccaac ggtgacgcgc gaaagatcaa ctccgtgggc
    36901 ttgcaacaga gccagttgtt ccagccccat ggtgctgaac gacgtatgcg tggagatcgg
    36961 gcgtccggtc tggttatgcg ccagcgcagc ggcaataaat accttctctt ccagcggcgt
    37021 aatctttcct tcgctggagc cgatctccgc gatgatcccg gctttcagct ccgtgccatc
    37081 gataccctgt tcaatttcat cgaccatctc ctgcgccagt tcctgcacgc tgcgggtcac
    37141 cacatgttcc gggaaaaacg catcctggta ataaccagta caggccacca cgttgatccc
    37201 ggtttcgcgc attacatcga gcataaattg cgcattgcgc cccatgtaac ggttggtcat
    37261 ctcaatcaca ttacgtacac cccgggtcat caggtcgttc atctcctggc aaatgaacgc
    37321 atactgatca aggcggcagt ccacgttgtt tttaaagccg gagagatcaa tatgcagatg
    37381 ctcatgggcc agggtgtaac ccgtcggatc aaaactcata gggtgtctcc tgcaaaccgg
    37441 agggaggaaa gaaaagagcg accattttgt ggcggtggcg cacggaaaaa ttcacacacg
    37501 gtagctccca catcagaaag cgtggtgcgc acaccgagct gcgtggcgac cagcccttgc
    37561 tgataaacca gcactggcac cacttcacgg gtatggtggc tgtgaccaat ggtcggatcg
    37621 ttaccgtgat ccgccatcac gaccaggcaa tcatctggct gcatcgcctc aacaagccgg
    37681 gcaaggttac ggtcaacgac ctgcaaacgt tcggcataac gtgcgacgtc ttctgcatga
    37741 ccagcgaggt cggtttcctg gatattgatg caaataaacg ccgtcggatg ggtgttaaat
    37801 tcgtcgaggg tgatatccat aatccgctgg ctatccacca ggttttgcca gctcacacca
    37861 taaggattgc tgacgatatc tgccacctta cccaccagca cggttggcac gcccgcttca
    37921 tacagttttt gcggcacctg cactttttca tcaacaccgt agcccatatg cacgacctgg
    37981 aagccgttgt cataagcgcc agaacgcggc gcattaatac caataaagcg cccttctttg
    38041 ctttctgcgg catcgagaat acgctgactg ttggttaaca ggccgccaaa tgtaatgacc
    38101 cgaccgacct gtacctggtc acgcacgata cgaccaattt tgattgcgtc gtcaaaagag
    38161 atcacagaga gattggcggt aatgttatag acttggccta aatccgcctc gagattatcg
    38221 ccaatcgcaa ccgcctgatt gaccgacaga aattgcagat catcgccacg gcgctccacc
    38281 tgccagccag cggaaactaa tgcctgctca acacggtcaa tcacatcgcg aaaaggcatc
    38341 cgcagcggcg gtaacgggcg cgtgcctaaa atttcctgat gccccataaa ggtatcgcca
    38401 ccttcatgtt gcagctctgc cacgccccag gttgcggaat ctgacggctg catatcgcct
    38461 ggcgcataac ccaatgtgtt gattagcccc agcttctcca gcgttggtag ctgcaaatgc
    38521 ggcaactggc tcaggatgtg accacaggta ttcgccccgg catcttgcgg gcgcaccagc
    38581 gtgacatctt tcattgcccc tacgccaaag ctatcaatca ctaacaccac aaatcgcgcc
    38641 atttatgcct ccagagggtt gccgaggctg tcataacgac caactatttc tggttcgccg
    38701 cgctgaatac cggacaccag caccacatcg ctacgggtga caaaaatctg cgtgcgaaaa
    38761 cagagcacca ctgcactgct caccggatac tcgcctgcca gcggcagggt gtagtcgata
    38821 ctgctgtcat ccacagtttt gagattggtt tcagtaatct tttgattttc tggcgtaaac
    38881 accagcgcat gttgcgcatg accacgacgg tagtaaccgc cgccgtagca gtagctgtcg
    38941 ccacggaaat gatgggagat ttcacttaac cagagcatcg cgatacgttc aggctgatcg
    39001 ccctgctggt ttgccggaat agtaccggtc agcgcatgac cgggttcggc atgagtcgca
    39061 ccgtattccg ccagcaatgg cagcgaagtg cagctggtcg ctgaaggtac gttcagttgc
    39121 tcaatcgcaa taccagattt cgccagttga tcccgtgctt gtaccagcgt gtgaagattc
    39181 ggtgttggca aaactttccc agcagcctca tcccaaagca ggcaagggaa atgggttagt
    39241 ccggctaaat gcagccccgg cagtttctta atttcagcaa tcacctcgtg cagcgaatgc
    39301 tgtacaaaac cgctctcctg ccccggataa agaaaatcat cgtcgctata aactttgagc
    39361 agcacaggct ggacccgtcc ggccttaacc gctgccgcag aaacttcccg cgctttgtcg
    39421 agagtaaaca cggtgatgat gtcggtgccc tgttcaacgg cgtcagcaac ctgatgacaa
    39481 gggatttgta ccagatgccc ctgatgcgcc actggcaaac cagcgcggcg catgactcgc
    39541 gcctctttgt aatccaccgc cacaatgccg ctgtagccta atgccagcaa tttttccgcc
    39601 agccacggat tacgaccaaa ttgcttggtc atcagataca gttcaatgcc gtaaagccgc
    39661 gccgtctcaa tgagccgctt accgttttcc agtatctgat ccacgtcgat cacccagctg
    39721 tccggggcga ttttgccctg ctgccacaaa cttagtgcgg cggaaatcag cgccgggttc
    39781 tggcgtttca atgcttctac aaacatcttg ttgcctcgtt tctgttcctt gaattaaata
    39841 ttcatttttt tgaatattta ggttgtgcga cgtggatttg tcgggagctt caaacctctt
    39901 cgttagcagc catccataat ccataaagat tggccagaaa atagccttct tcacacgggt
    39961 taacttccag agcgaattcc ttcaacaact cctgatgcaa ttgcacaacg gcttgccagt
    40021 ggctggattg cgccagttcc gccagcaact cgttatccag cggctctatt tcttcaccac
    40081 ggcgactgcg catcagtgca ctcgccatat gtgtcatcgc catcgttcct tgctcactgc
    40141 gcaccggcag atggcactct ttttccagta cgttgacgac ctgcatcatg cctttgcaga
    40201 cgtccttatc aataacgcct gcctcgcaaa gcaggttgag tctggtttcc atatcagatg
    40261 cctttcgttt aataactggg ttcctgttcc ccactcacca cccgttgttg atgggcaagc
    40321 agggcgtgtt tatctacaac agcacgcagc aattgttgca ttgggtaatc atcgactcgc
    40381 gtcaggacca cggcttcggt ggcctgtaaa aatcgcggat cgtctgttag cggcgtcgcc
    40441 tctaatccca gcatggtcag ttcgttttcc gccaccacgt tccagatcac cgcatcgaca
    40501 tcgcctttaa caatgcgttg taaactctcg tgataagaga gatcgactcg ttccacatca
    40561 ctatcgccaa aaaagacatc ggtcatgatt ttctgatccg ccgaacgact atcgagacct
    40621 actcgcttaa catttccaga ctcacctttg cggcatatca gttgatgttc accaacatac
    40681 gtatgcgggc caagctcgag ggcgatgcac aaattgtttt ggctgagata actttccgcc
    40741 gccaggcggg aaactactgc catgtcatag acaccgttaa gtaaacactc cacgcgaata
    40801 tccgcgccgc gcatatgcgc atagtaaaaa ggaatgccat caaactgggc tttcaatccg
    40861 ctcgctaaac cttcgtacaa gcgggtatag ggcaaaggca tcgcacatac cacgttgttg
    40921 atatccacat gactcagcaa tgctttgtta tccatctcga caagataact gccattgcgc
    40981 ccacggcgtt caatccgtat cgctccgctt gattccagcg ttttcaacgc ggcctgcgtc
    41041 aggccaaccg atgaacggca ttcagttgcc agctcatcta tggttttcag tcgattaccg
    41101 catttttcac ccaacaaata acgggccagt gtcgtgatga cgacgccttc ttttttgata
    41161 aacgttcgac gcataatata ttttcaataa aatgaatatt tatatcttca ttaaaatgaa
    41221 gataagtggc gacgacgaga tgtggaaacg gcgaggcgct tcacattttt tcgggttgtt
    41281 gcgaaaagag gataaaaaaa ccgggtttcc ccggtttcag agtgatgacg aagtgattta
    41341 ttcgacaatg cgagcaaagg gtaattccca ctggcgcttg cctgctcccc tcaccctaac
    41401 cctctcccca aaggggcgag gggaccgtcc gagcgttttg atattacggc ttcgccacaa
    41461 aaccaatcgc ttcgtacacc gctttcagcg tacgggaagc gtgtacactg gctttttctg
    41521 cgccgtcttt catcacctgt tgcaggaagg cttcatcgtt gcggaaacgg tgatagcgtt
    41581 cctgcaattc agtcagcata ccggaaacgg catcagccac ttcacctttc agatgaccat
    41641 acatcttgcc ttcgaactgt ttttccagtt ctgggatgct ctggcccgtt accgcagaaa
    41701 ggatatccag caggttggaa acgcccgctt tgttctgcac atcgtagcga actaccggcg
    41761 gctcgtcgga gtcagtgacc gcgcgtttga ttttcttcac taccgatttc ggatcttcca
    41821 gcaggccgat aacgttattg cggttatcgt cagacttgga catcttcttg gtcggctcca
    41881 gcagcgacat tacgcgcgcg ccagatttcg gaataaacgg ctccggcacc ttaaagatct
    41941 cgccatacag cgcgttgaaa cgctgggcga tatcgcgact cagttccagg tgctgtttct
    42001 ggtcttcacc caccggtacc agattagttt gatacagcag gatgtccgca gccatcagca
    42061 ccggatagcc aaacagacca gcgttgatgt tctcggcata acgcgcagat ttatctttaa
    42121 actgcgtcat gcgactcagt tcgccgaagt aggtatagca gttcagtgcc cagcctaact
    42181 gcgcatgttc tggtacgtgg gactgaacaa aaatggtgct tttctcagga tcgataccac
    42241 aagccagata caaggccagc gtatccaacg tcgctttacg cagcttctgt gcatcctggc
    42301 gcacggtgat cgcgtgttgg tcaacgatac agtaaacgca atggtagtca tcctgcatgt
    42361 ttacccactg acgcagcgca cccatgtagt taccaatggt caattcacct gagggctgtg
    42421 cgccactaaa aacgatgggc ttagtcattt ttcgattcct gattttcgct atgcggaagc
    42481 cctaatgcgg gcagaaggtc atttatagac tgataaatta catcaggctg gctgagatcg
    42541 atagcctcgc cgtagttgta tccgtaggtt aagccaactg atgggcaacc tgccgctttt
    42601 gccgcctgaa tatcattccg tgagtcgccg acaaacagca tctgttgtgg ggcaattccc
    42661 atccgctcag ccaccagtaa cagcgggtcc ggatgcggtt ttttgttttg cacatcatcg
    42721 ccgccgataa ccacgctaaa gtatttggca atatctaagg cttcgagcag cggcgcgacg
    42781 aacggcgtcg gtttgttggt gaccaagcct agcggcaggc ctttagcctg caacgcgccc
    42841 aacgtatcgg caacgtgcgg gaacaaaaac gtcccctctt cggcaacctc gccatagtag
    42901 cgatcgaaca gtttacgcag aatacgtacc tgttcttctg ccggaatgtc gtcatcaacg
    42961 ggcggtttcc ccattgtttt acgcagagtc gcacgttcct gacgcgccca ggtcaatgcg
    43021 cgctccatca gaacatctgc gccgttacca atccaggtaa taacgcgttc ttcacctgcg
    43081 acgggcaact ccagcgcata cagcgccata tctaccgcag cagcaagacc aggagcactg
    43141 tcgaccagcg taccatcaag atcaaaagcg acgccgcgaa tatcttcaaa cttattcatg
    43201 acttaccttt gccagttcac tgcgcatttc atcaatgact tttttatagt ctggctggtc
    43261 gaagattgcc gaaccggcga cgaacatatc cgcgcccgcc gcagcgattt cgccaatgtt
    43321 gttcaccttc acgccaccgt ccacttccag acgaatgtca aagccagact cgtcgatacg
    43381 gcgacgtact tcgcgcagtt tatccagtgt ttgaggaatg aaagactgac cgccgaaacc
    43441 agggttgacg gacatcagca ggatcacatc cagcttatcc atcacgtaat ccagatagct
    43501 cagaggtgtc gccgggttaa ataccagacc cgctttacag ccattttctt taatcagttg
    43561 cagcgtgcgg tcaacatgct cggaggcttc tggatgaaag gtaatgatgc tggcaccagc
    43621 ggcagcgaaa tcaggcacaa tgcgatcgac gggtttcacc atcagatgta cgtcgatagg
    43681 ggcggtaatg ccgtagttac gcaaggattt cagcaccatt ggcccaatcg tcagattggg
    43741 aacatagtgg ttatccatga cgtcaaaatg cacgacatca gcgccagctg ccagggcttt
    43801 tgcggtatct tcacccaggc gggcaaaatc agccgacaga attgaggggg caatcaaata
    43861 ctgtttcatc cgcttctcct tgagaattat tttttcgcgg gtgaaacgac tcctggtttg
    43921 tacaaagcca gcagttcgtc caccttttta cgtgtgccgc cgttgctgct tatactgcgt
    43981 cgaactttga cgacatgcaa ttttgcgcgc tgataccact cacgcgttaa catcgtatcg
    44041 tgattggaga tcagcactgg aatatggcgc tcaaccagac cttcggcgat ctccgccaga
    44101 tgcgcttgtt gttcaagcgt aaaactgttt gtgtgatacg ccgtaaagtt ggcggtcgca
    44161 gacagcggtg cataaggcgg atcgcaatag acgacggatg catcatctgc gcgctccatg
    44221 ctatcggcgt aagactcaca atagaaaaag gcattctgcg ctttttcagc gaagtgatac
    44281 aactctgctt ccgggaaata gggttttttg tagcggccaa acggcacgtt aaactcaccg
    44341 cgcagattgt aacgacacag gccgttgtaa ccgtagcggt tcaaatataa aaacagtacc
    44401 gcccgacgga acggatcctg gcttttgttg aactcttcgc ggaactgata gtaaacctcg
    44461 gcgcaatttg tttcgggaac aaacagctcg cgtgcggcct gtacgtactc atcagtacgc
    44521 atcttcacaa tgttatagag actgatcagg tcgctattga tatcggcaag gatataacga
    44581 gaaaagtcgg tgttgagaaa caccgacccg gcacctacaa aaggctcaac cagacattcg
    44641 cccttgggca aatgccgttt aatatcatca agcaggggat acttgccccc tgcccacttc
    44701 aaaaaagcgc gatttttctt catgccgatt aactaattac accttctccg gctgtggaga
    44761 aagctccgac agcatccagc gcttcaggca gctcccgcaa cgataaacca ttgcgggaga
    44821 taaccttgat tacttcagat cggcctgtac ctgacgcagc ggtttcgccc acgggttttt
    44881 ggcctgaaca tctgctggca atgtagatac cgcttttttc gcctcttctt tcgaagcgta
    44941 cacgccagaa accaggacat accacggctg accattacgc gtcgtttcat agacaacgta
    45001 gtttttcaga ttctctttct tcgcccaacc gttcaggttg tcgtagttag aggaactgct
    45061 cagctgcaga gtgtaatggc tggacggtgc cgatttcaac gaaccaacat tacctgcggt
    45121 cttccctcca gcggctggtg ttgccgtggt ttgcgccggg gatgccgtct gtactggagc
    45181 cgtagtcgcc gtctcttttg gtgctggcgt agaagtcgca gccggtgcct tcgtgctcgc
    45241 cacaggggca gctggttcag tacgcttcgg cgtctgtgct accggcttcg gctccgtttt
    45301 cacggttgct tgcggttttt tcggttcaat caccgcctgc tgacgaactg ggcgcgtagt
    45361 ggacggacgt tcagcggttt gcgttttcgc cgagtcacgc gatgcattgc cattgcgaac
    45421 aggcgcaacc gttgcgggtt cagtcggcaa cgtggaattg accgccacat tgttcagctg
    45481 ttgctgattt tgtggctggg tcagcgcatt gttcaggtca ccctgcactt caacacgttg
    45541 ttgaccatcc gttgccaccg gggtttgccc ttgagtcggc gtagaagaga tcggtggcag
    45601 cgaaacatcc tgctgagtat tttccgcaga cgtggttccc ggcgctggct gcacgccatt
    45661 cgcctgatcg gtcgcattgc cagcaagatc aatactcttc tcgccagacg cggtttgatc
    45721 gctggaagtg gtcgaggggg cttttagcgc agaaccgata ccgatgatca acagcagtag
    45781 aaccagaatg ccgacgccca tcatcatata ctgacgagaa gcgggtttac tggctgcttt
    45841 tttgcgctta cgcggacgac gctctacgcg ctcttcatcc acggtttcat cttcggattc
    45901 gtcaatttct tcttcgattt ccggttcctc attgcgctct ttttgcgcac gagtcggacg
    45961 gcgatcgtca gtgtcatcaa gttcaatatc atcaaaattg atctgcggtt cgccacgttc
    46021 agtacgctca gaacgttcag aagattgacg agaacgacca gtacgacgat cgctgggatc
    46081 gggtttcagc tcgtcttctg gtttgaattc atccatttaa caccccacta aaaggttaat
    46141 gcttaccacg ttgcaattaa cctgaagcta atagaccgct tgataagcgg cctgaccttt
    46201 cttgttgtta cgctgattga caatcggcaa tggcgttaag aacaagctcg tgcgaaacgc
    46261 cgctgcgaac ttcactctta ccaattgcca acggaagaat taagcgcatc tctcccgcaa
    46321 ggactttctt gtcacgcagc atatgcggta aatacgcctg cgcggacatt tcgcgcggcc
    46381 cattgaccgg taacccagcc cgcgtgagca gggttataat acgctgcgtt tcggcagaac
    46441 taaactgccc gagacgttcc gacgtccgcg ccgccatcac catacccgca gcgaccgctt
    46501 caccatgtaa ccaattgcca taccccattt cagcttcaat ggcatgacca aaggtgtgtc
    46561 ccagattcag taaagcacgt aacccggttt cgcgctcgtc ggcggcgaca acttctgcct
    46621 tcagttcaca acaacggcga atacagtacg ccattgccgg accgtccaga cgcaacaacg
    46681 catccagatt ctcttccagc cagttaaaaa acgcaccgtc aagaataatg ccgtatttga
    46741 tgacttctgc cagccccgac gctaactcac gcgggggaag cgttttcaga cagtcgagat
    46801 ccaccaccac tgaagcaggc tggtagaacg cgccaatcat gtttttaccg aggggatggt
    46861 tgaccgcagt tttgccgcca acggaggaat cgacctgcga cagtaacgtc gtcgggactt
    46921 gaatgaaacg aacaccgcgc tgataactcg ccgccgcgaa gccggtcaga tcgcccacta
    46981 cgccgccgcc aagcgccacc agcgtagtat cgcgaccatg cggcttttgt aacaacgccg
    47041 taaagacggt atcgagtaca gctaggcttt tatactgctc gccgtcaggg aggataacgc
    47101 tatcgacgtt aacacccgcc tgttcaagta cgccgcggac cttatcgaga tacagaggag
    47161 ccagggtttc gctggtgacc aacatgacct gctcgcccga tttcagcggt aagaatgaag
    47221 ctggttcatt aaacaaacca gatgcgatgg taattgggta actacgttcc ccgagagtaa
    47281 cgacaatcct ctccataacg cgacatccac cttaattact gtacccgcag acgagtgtat
    47341 ataaagccag aattagttgc tttccagcat gtgaataatc tggtttgcaa ccactttagc
    47401 gctttgatca tcagtacgaa tggtcacgtc ggcaatctct tcatacagcg gattgcgttc
    47461 attggccaac gcttccagaa cttcacgcgg cggtgtttca acgtgcagca acgggcgttt
    47521 tttatcacgc tgcgtgcgtg caagttgctt ttcgatggtc gtttcaagat aaacgacaac
    47581 gccacgagcg gaaagacggt tacgcgtttc acgggatttc acagagccgc cgccagtagc
    47641 cagcacaata ccctgtttct cggtcaactc attgatgacc ttttcttcgc gatcgcggaa
    47701 gccttcttcg ccttctaaat cgaaaaccca gcccacatca gctccggttc gtttctcaat
    47761 ctcttgatcg gaatcgtaaa attccatatt gagctgttga gctaactggc gcccaatagt
    47821 gctttttccg gcacccatag gcccaaccag aaagatattg cgtttctctg ccattttttc
    47881 ggtactacta agactattcg ttaatgataa acccgcttcg ctcagagagc gccgcaggac
    47941 atgaactgaa acctcataag atattgcgag agtcagactg aaaattatct caatactcca
    48001 gcgggtttgg caactgaata aatcacccat ccctctgcat atctggtcgc tgcaagcgcg
    48061 ctgccttgct accaccgctc tggcgataaa tcaccgggta agattagcgt aaaaaagaca
    48121 gcaaaatgcc gcctgaatga taaatcatca tcatggaacg caggcagcga agagcaccaa
    48181 ctctcaaatc ggtactcctt gtatgctaaa tacctgcgcg cgtcaaatag atgaaacacg
    48241 ttcagcataa aaacattacc gcttttacgg ctgtttactc actggaaacc agtcgtggcg
    48301 tgataaacac cactaactcg cgtcgttcat cttcttttcc gtcatgacga aataattgcc
    48361 cgaaccaggg aatgtcgcca agcaacggta cgctatcctg acccgattta tttttacggg
    48421 taaaaatgcc gcccagcgcc aacgtttctc cgctttttac ctcgacctgc gtttcgatct
    48481 cctgcttatc aatcgccagc acttcgccat cggcctgctg tagcacctgc cccggaacgt
    48541 tctggctgat gtgtaatttc agccggatgc gacctttttg taacaccgtg ggcgtgacct
    48601 ccatccccag gacggcctct ttaaattcca ccgacgtcgc gccactttcc ccgctggaaa
    48661 cctgatatgg aatttcgctc tcctgtttaa tgctggcagg ctgaagatgt gaggccagca
    48721 gacgcggact ggcgataata tccagctgct gtttttgttc gagcgcggaa agctcaagat
    48781 ccagcaagcg tccgttgatg cgcccaatgt taaaaccgac atgcgttgtc gccgtcgcta
    48841 cggagaggtc gctaccaagc gtggtgactt gcccaacgcc accagcgtgt tgcgcatcgg
    48901 ccagcgtcca tttcacgcct aactcacgca aacttttttc attaatggtg acaatatgcg
    48961 ccgacagctc aacctgcccg accggcagat ccatttgcgc tacccactgt tcaagcgcgc
    49021 ttaacgccgt tttgttatct cgtagcaaaa ggcgattggt gcgtttatcg acggtcatac
    49081 tccctttggc actcagtagc ttctcccccg ctttcgccag ttctcccgcg tcggcgtatt
    49141 gcagggttat actgcgattt tccagcggca gatttgcctg cgcccgcgcc tgctccgcct
    49201 cctggcgggc gatattgtca ttctgccagg caatggaatg cactgagaga atgttgcctt
    49261 cctgccgcgt tatcagtccg gcgcttttca ctacagtttg tagtgcctgc ttccagggaa
    49321 catctgttag atgtaacgac accgtaccgc tgacgtctgg cgacacgacc aggttcaact
    49381 tctcctgttc agccagcgcc tgcaacacct gagctaccgg aacgtcatcc accatcagcg
    49441 tcactttttg cggctttgcc gcctgtacgc cgggtatcag catcaacagt agtgcggcta
    49501 tccattgctt catttgtatc tccttgccgt tgccacaacc attgtggcgg ttcgcagttt
    49561 gtcccggtac ccagcgttag tacgtctggc gtcagctgta aaattgtcca gccgttttcc
    49621 agcacatcgt tttgctgcac ccgtcgccat ttcttttgcc cgtcttttat tacaccgatg
    49681 atgcgctcgc ctcgccctac catcccctga tagcgccact ggctaagttc gctaatccgg
    49741 catagatctt ccggcgattt aaaagggtca cgcataccgg ttaaaaggca caatgcaata
    49801 cctgccaaca accagcgttt aaccctcatg cggcatctcc agttgtagcg tgaacaaaag
    49861 atcatcacct tccacgctta acgaaaaacg gctcacgctg acgttgcgct ctgccagccg
    49921 tgtaaatgcc gacggcactg cttcccacag cgttttcaac gccaactcgc ctccctgcgc
    49981 ggatggatgc caggaaacca gttgcgcgcc ggataactga aaatccagtg gcgaaaaggg
    50041 cagcgttttt tcctcgctga agggagtggt gtctaccagg cgatacagtg cggcccactg
    50101 ctggtgatga cttgcccgta gtcgaattaa tgcgtcgcgc tcttcatggt gtgtcgatga
    50161 tagaaaaatg agcgtaacta acatcagcaa ccagaatgcc cagcaaaact ggcggaggcg
    50221 gggtgatgtg gcgaaccacc agtcaaagaa catgttcatc gctaaccttc cttgttaact
    50281 gatactcaaa ttgccagcgt ccctgcgcat cctgctgcgt ggctccccgc tgattgagat
    50341 gaaaagacgc atcctggcgg agtgacgttt ctagtgcgtt taacgcggta atgcttgttg
    50401 tcagcccctt gatctccagc gttccctgct gccagcttat cgttgtcagc caggcgtgct
    50461 cgggtaaaag cgccgccagt gcttccagcg cagattgcca gtcgcgggta aattgtcgct
    50521 ggcgctgccg ttgcgaacgc tgttcgcgta actgctgttg ctccagcaaa cgtggcttcg
    50581 ttatctgtaa gctgcgggcg agttgttgtt ccgcctgaag caaaactgcg tctatgcgcg
    50641 cttcggcgct gcctgtcaga cgcagtatta gcgttatccc gacggccagc agcagaggcg
    50701 caacgaacat cagcaaccag aaacgcagaa aagcggtccg gcgttgctgt cgccagggca
    50761 aaaaattaat tggcgggttc atcagtacgc cttcccaagc gccagcccca gcgcgatggc
    50821 aaagtctcca ccaggcggcg gtagcggcgg ctgacgaaca gaaacggcct cccaggggtc
    50881 aaatccgcct tcgccacata tcgcgacgct gtcgggatca acggataacg ctgccgccag
    50941 ctccttcgcg ctagtcatcc ccaccgccag tttgcgcccc cagctatagc gcgtcgccca
    51001 cagccactgt tcgttatcac gccaggccag acattgctga tgagaaggta aaaaaggcag
    51061 gaatcgctgt aatgcactgg catccggggt gatcgcacta acatgaacac gcaacctttc
    51121 tgccagcgta agcagcgttg ccagctcttt gctttgcgcg gcagtcacgt tataagcggg
    51181 gctgagtgag tcttcgctgt aatcgaagcg cagggagtcc ggatccatat ccagctcgcg
    51241 ggccatcgtc cctgacagcc aggccgtttg ctcccgctca ccaagggaca tcgacgggcg
    51301 cggaaatgac cgctgtaatg tgcgactggc gggaaacgcc aacataatgt gatgacgctg
    51361 cggcagttcg cgactccacg gtaacaacgt tttagccagc tgctgcgcat caacaatccg
    51421 cccatctttg ataatgtcgt gcgccagcgg caaccgccac cagcgttgca aaaagcattc
    51481 ttttgcgccc cgcacgatcg caaccgctgc cgcttcttgc tgttgtaaat gcaaaccaat
    51541 ttgccagatc ttaaatgtca ttgtgatgat ctccttatca cccgtcactc tgacgggtat
    51601 atcaatgcgt ctggcttgcc tttatactac cgcgcgtttg tttataaact gcccaaatga
    51661 aactaaatgg gaaatttcca gtgaagttcg taaagtattt tttgatcctt gcagtctgtt
    51721 gcattctgct gggagcaggc tcgatttatg gcctataccg ctacatcgag ccacaactgc
    51781 cggatgtggt gacattaaaa gatgttcgcc tgcaaattcc gatgcagatt tacagcgccg
    51841 atggcgagct gattgctcaa tacggtgaga aacgtcgtat tccggttacg ttggatcaaa
    51901 tcccaccgga gatggtgaaa gcctttatcg cgacggaaga cagccgcttc tacgagcatc
    51961 acggagttga cccggtgggg atcttccgtg cggcaagtgt ggcgctgttc tccggccacg
    52021 cgtcacaagg ggcgagtacc attacccagc agctggcgag aaatttcttc ctcagtccag
    52081 aacgcacgat gatgcgtaag attaaggaag tcttcctcgc gattcgcatt gaacagttgc
    52141 tgacgaaaga cgaaatcctc gagctttatc tgaacaagat ttaccttggt taccgcgcct
    52201 atggtgtcgg tgctgcggca caagtctatt tcggtaaaac ggtcgaccaa ctgacgctga
    52261 acgaaatggc ggtgatagcc gggttgccga aagcgccttc cacctttaat ccgctctact
    52321 caatggatcg tgccgtcgcg cggcgtaacg tcgtactgtc gcggatgctg gatgaagggt
    52381 atatcaccca gcaacagttc gatcagacac gcactgaggc gattaacgct aattatcacg
    52441 cgccggagat tgctttctct gcgccgtacc tgagcgaaat ggtgcgccag gagatgtata
    52501 accgttatgg cgaaagtgcc tatgaagacg gttatcgcat ttacaccacc atcacccgca
    52561 aagtgcagca ggccgcgcag caggcggtac gtaataacgt gctggactac gacatgcgcc
    52621 acggctatcg cggcccggca aatgtgctgt ggaaagtggg cgagtcggcg tgggataaca
    52681 acaagattac cgatacgctg aaggcgctgc cgacctatgg tccgctgctg cctgccgcag
    52741 tcaccagcgc caatcctcag ggagcgacgg cgatgctggc ggacgggtcg accgtcgcat
    52801 tgagtatgga aggcgttcgc tgggcgcgtc cttaccgttc ggatactcag cagggaccga
    52861 cgccgcgtaa agtgaccgat gttctgcaaa cgggtcagca aatctgggtt cgtcaggttg
    52921 gcgatgcatg gtggctggca caagtgccgg aagtgaactc ggcgctggtg tcgatcaatc
    52981 cgcaaaatgg tgccgttatg gcgctggtcg gtggctttga tttcaatcag agcaagttta
    53041 accgcgccac ccaggcactg cgtcaggtgg gttccaacat caaaccgttc ctctacaccg
    53101 cggcgatgga taaaggtctg acgctggcaa gtatgttgaa cgatgtgcca atttctcgct
    53161 gggatgcagg tgccggttct gactggcagc cgaagaactc accaccgcag tatgctggtc
    53221 caattcgctt acgtcagggg ctgggtcagt cgaaaaacgt ggtgatggta cgtgcaatgc
    53281 gggcgatggg cgtcgactac gctgcagaat atctgcaacg cttcggcttc ccggcgcaaa
    53341 acattgtcca caccgaatcg ctggcgctgg gttcagcgtc cttcacccca atgcaggtgg
    53401 cgcgcggcta cgcggtcatg gcgaacggcg gcttcctagt ggacccgtgg tttatcagca
    53461 aaattgaaaa cgatcagggc ggcgtgattt tcgaagcgaa accgaaagta gcctgcccgg
    53521 aatgcgatat tccagtgatt tacggtgata cgcagaaatc gaacgtgctg gaaaataacg
    53581 atgttgaaga tgtcgctatc tcccgcgagc agcagaatgt ttctgtacca atgccgcagc
    53641 tggagcaggc aaatcaggcg ttagtggcga agactggcgc gcaggagtac gcaccgcacg
    53701 tcatcaacac tccgctggca ttcctgatta agagtgcttt gaacaccaat atctttggtg
    53761 aaccaggctg gcagggtacc ggctggcgtg caggtcgtga tttgcagcgt cgcgatatcg
    53821 gcgggaaaac cggtaccact aacagttcga aagatgcgtg gttctcgggt tacggtccgg
    53881 gcgttgtgac ctcggtctgg attggctttg atgatcaccg tcgtaatctc ggtcatacaa
    53941 cggcttccgg agcgattaaa gatcagatct caggttacga aggcggtgcc aagagtgccc
    54001 agcctgcatg ggacgcttat atgaaagccg ttcttgaagg tgtgccggag cagccgctga
    54061 cgccgcctcc gggtattgtg acggtgaata tcgatcgcag caccgggcag ttagctaatg
    54121 gtggcaacag ccgcgaagag tatttcatcg aaggtacgca gccgacacaa caggcggtgc
    54181 acgaggtggg aacgaccatt atcgataatg gtgaggcaca ggaattgttc tgattaaaaa
    54241 ggcgcttcgg cgccttttca gttggctgac aaagtgcatt tgtttatgcc ggatgcgggt
    54301 gaacgcgtta tccggctaac aaaatcgtga aaaatcaata tattgcagga accccataag
    54361 cctgataagc gttgtgcatc aggcaaactt cacgcattta cactcgcccc tgccctttca
    54421 accattcgcg cacgaggaac agcgcgctga cattacgcgc ttcgttgaag tcagggtctt
    54481 ccagcaaatc catcatatgc gccagcggcc agcgcacctg tggtagcggc tctggctcat
    54541 cgccttccag cgattccggg tagagatctt gcgcgaccac gatattcatt ttgctggaaa
    54601 agtaagacgg tgccatgctg agcttcttca aaaaagtcag atcgttcgca ccaaatccaa
    54661 cctcttcttt tagctcgcgg ttagcggctt cgtagacgct ttcacccgga tcaattaatc
    54721 ctttcgaaaa acctaattcg taggattcag ttcccactgc gtattcgcgg atcaggatca
    54781 ggtgatcatc cacaatcggc acaatcatca ctgcttcccg gttggttgga cgcattcgtt
    54841 cataaacacg ccgcacgcca ttgctgaact ccagatccac gctctcgacg gtaaacagtc
    54901 gggaacgggc tacagtttca acattcagaa tggtgggttt ttgtaatgat ttgttcatcg
    54961 tgggatctat gctgtgaaat cagcggttat tgtgcgatat cggacacgct ttcggcaatg
    55021 tgaattgcat gttatttaca tttatgtaac ttaataaata atcgtcctca aatcaaatta
    55081 aaagtcaata ggttgaaata actccaggaa tttgctgata ttccgccttc agagggtttg
    55141 ctatgatcag cggttactgt gatgtgctta atgatgctca agttaagctc cacgcttgcc
    55201 gatagccaac cgcagaatca tgtattgtgt ccggtgcgac tgaccacgcc tgacagacta
    55261 agtaagatgg ggaaagcatg agcaccattg tgattttttt agctgctttg ctggcctgct
    55321 cactacttgc gggatggctg ataaaagtgc gatccagacg gcgtcagctg ccctggacca
    55381 acgccttcgc ggatgcgcaa acgcgtaaac tcacacctga agaacgtagc gccgttgaaa
    55441 attatcttga gagcctgacg caggtattac aggtgcctgg cccaacggga gctagcgcgg
    55501 caccgatctc tctggcgctg aatgccgaaa gcaacaacgt catgatgctg acacacgcta
    55561 tcacacgtta cggcatctct accgacgatc cgaataaatg gcgttactac ctcgattcgg
    55621 tagaagtcca cctgcccccc ttctgggaac agtacatcaa cgatgagaat accgttgaac
    55681 tgattcatac cgattcgctg ccgttggtta tttcactcaa cggtcatacg ctgcaggagt
    55741 acatgcagga aacgcgcggc tatgccttgc aacctgttcc gtccacgcag gcgtcgattc
    55801 gcggggaaga gagtgagcaa atcgagctac tcaatattcg caaagaaacg catgaagaat
    55861 atgcgctgag tcgtccgcgc gggctgcgtg aagcgttgct gatcgtcgcc tccttcctga
    55921 tgttcttttt ctgcctgatt accccggatg tatttgttcc gtggctggca ggcggcgcat
    55981 tactgctgct gggcgcaggt ctgtgggggc tattcgcgcc cccggcaaaa tcctccctgc
    56041 gagaaattca ttgtctgcgc ggtacacccc gtcgttgggg attgtttggc gaaaacgatc
    56101 aggaacagat caacaatatt tcactcggta ttatcgacct ggtctatccc gcacactggc
    56161 agccatacat tgctcaggat ctcggtcaac aaaccgatat tgatatctat ctcgaccgcc
    56221 acgtagtgcg tcaggggcga tatctttcgc tgcatgatga agtaaaaaac tttccgttac
    56281 agcactggct gcgcagtacg attatcgctg cgggctcgct gctggtgctg tttatgctgt
    56341 tattctggat cccgctggat atgccgctga aattcactct ctcatggatg aaaggcgcgc
    56401 agaccattga agccaccagc gtaaaacaac tggctgacgc tggcgtgcgg gtaggcgata
    56461 cattgcgtat tagcggtacg ggaatgtgta atattcgcac ttccggaacc tggagcgcga
    56521 aaaccaattc acctttttta ccgtttgact gctcgcagat catctggaat gacgcccgct
    56581 cattaccatt accagaatct gaactggtca acaaagcgac ggcattgact gaagcagtta
    56641 atcgccagct gcaccctaaa ccggaagatg aatctcgcgt cagtgcctca ttgcgttcag
    56701 cgattcaaaa atccggcatg gtattgcttg atgattttgg cgacattgta ctgaagacag
    56761 cggatttatg ttctgccaaa gatgactgtg tgcgactgaa aaatgcgctg gtcaatctcg
    56821 gcaacagtaa agactgggac gcgctggtaa aacgcgccaa cgccgggaag ctcgatggcg
    56881 tgaatgtgtt attacgcccg gtgagtgcgg aatcgctgga taacctggtg gcaacctcca
    56941 ccgcgccgtt tatcacgcat gaaacggcgc gagcggcaca atcactaaac agtccggccc
    57001 ccggcggatt cctgattgtc agcgacgaag gcagcgattt tgttgatcag ccctggcctt
    57061 cggcatcact ttacgactac ccgccgcaag aacagtggaa cgctttccag aaactggcac
    57121 aaatgctgat gcatacgccg tttaacgccg aaggtatcgt cacaaaaatc ttcactgacg
    57181 ccaatggtac gcagcatatt ggccttcatc cgatcccgga tcgttccggc ctgtggcgct
    57241 atctcagcac cacattgctg ctactgacga tgctgggtag cgccatttac aatggcgtac
    57301 aggcctggcg tcgttaccag cgtcatcgca ctcgcatgat ggagattcag gcctattatg
    57361 aaagctgcct gaacccgcaa ctgatcaccc cttcagaaag ccttatcgaa taacacgttt
    57421 gcgcggcagg ttatgctacc ctgtcgcgca aattgcttca ctctggagat ttccctcatg
    57481 catatcaaca ttgcctggca ggacgtagat accgttctgc tggatatgga cggcacgttg
    57541 ctcgacctcg ccttcgataa ctatttctgg caaaagctgg tgcctgaaac atggggcgcg
    57601 aaaaacgggg ttacgccaca ggaagcgatg gaatatatgc gccagcaata tcacgacgta
    57661 cagcatacgc taaactggta ctgtcttgat tactggagtg agcaactggg tctggatatc
    57721 tgtgcgatga ccaccgagat gggaccgcgc gccgtactgc gtgaagatac cattccgttt
    57781 cttgaggcac tgaaagccag cggtaagcag cgaattttgc tcaccaatgc tcatccgcac
    57841 aacctggcgg taaaacttga gcatacgggt ctggacgcac accttgattt attactttcc
    57901 acccacacat ttggttatcc gaaagaggat cagcggttat ggcatgcggt ggccgaagct
    57961 acgggtctga aagctgaaag aacgctgttt attgatgaca gcgaagcgat tctcgatgct
    58021 gccgcgcaat ttggtattcg ttactgcctc ggcgtgacta atcctgattc cgggattgcc
    58081 gagaaacagt atcaacgcca tccgtcactg aatgactacc gccgcctgat cccctcgcta
    58141 atgtgaagga gacgccatga aagagaaacc tgctgttgag gttcgactgg ataaatggct
    58201 atgggctgcc cgtttttata aaacccgcgc gctggcccgt gaaatgattg aaggcggtaa
    58261 ggtgcattac aacgggcagc gcagcaagcc gagcaaaatc gtcgagctga atgccacgct
    58321 cactctgcgc cagggaaatg acgaacgcac ggtgattgta aaggcgatta ctgaacagcg
    58381 tcgccccgcc agcgaggcag ccttgctgta tgaagagact gcggaaagtg tagagaaacg
    58441 cgaaaaaatg gcgatggcac gtaaacttaa tgccttaacc atgccgcacc cggaccgacg
    58501 cccggacaaa aaagagcgcc gcgacctgtt acgatttaaa cacggcgaca gtgaataact
    58561 gtcacctgca agagagatga ttatgccgca acatgaccaa ttacatcgct atctgtttga
    58621 aaattttgcc gttcgcggcg aactggtaac cgtttcggaa accctgcaac agatccttga
    58681 gaaccacgat tatccgcagc ccgttaaaaa cgtgctggca gaactgctgg ttgcgaccag
    58741 cctgttaacc gctacgctga agtttgatgg tgatatcacc gtacagctgc agggcgacgg
    58801 tccgatgaat ctggcggtta ttaacggtaa caataaccag caaatgcgcg gtgtggcgcg
    58861 cgtgcagggc gaaattccag aaaatgccga cctgaaaacg ctggtcggca atggttacgt
    58921 ggtgatcacc attaccccga gcgaaggcga acgctatcag ggcgtagttg gtctggaagg
    58981 tgataccctg gcggcctgcc tagaagatta ctttatgcgt tctgaacagc tgccgacgcg
    59041 cctgtttatt cgcaccggcg acgtagacgg caaaccggct gcaggcggta tgttgttgca
    59101 ggtaatgcct gcgcaaaata cccaacagga cgactttgac cacctggcga cgctaaccga
    59161 aaccatcaaa accgaagaac tgctgacctt accggcaaac gaagtgttgt ggcgtttgta
    59221 tcacgaagaa gaggtgacgg tttacgatcc gcaggatgtg gagttcaaat gcacctgctc
    59281 gcgtgaacgt tgcgccgatg cgctgaaaac gctgcctgat gaagaagttg atagcatcct
    59341 ggcggaagat ggcgaaattg acatgcattg tgattactgc ggtaaccact atctgttcaa
    59401 tgcgatggat attgctgaaa tccgcaacaa cgcgtctccg gcagatccgt aagttcatta
    59461 atgagtatgt ccggcagaga gggctctgcc ggattttacg acttaatggc ctgatgtgct
    59521 gcgcttacca gcgcctatga aataaatcgc aaattatttt ggtcagatac agcgtcgtat
    59581 ccgataaaag cgttgtcact tacttaagtt tatttccagc gtttcaccat ctaccgtcag
    59641 atcgcgccac gctttcggcc tcgtccccag ttttccatcc ttcgcagctg tgagtaattt
    59701 ttctttcgtt atttcgttcg gaagtaaact catatcaagc gcgtcccatt cttttctgtc
    59761 tgagtcaaag ccatagacaa tgactctgtc atcattaaaa gcaaacagga tccgctccgc
    59821 ctgaccatca ctattcagat cttgctcgac cagtacgcag gcgtcttttt caatacaggt
    59881 catcacgtta tagcgatcct ggattaaggc cgaccagaat gtcgcatccg gtttaacaga
    59941 accaggggca attaacacat tttcggcgaa tactttttcc gatacctgtt cctgaagatg
    60001 ttgctctcca tctaacgcca tcagcagatc ccgcgcgcgt ttcgggtctt tcataaaccc
    60061 ggcatcgctt ttcagcgact caagcgccgc acgtccatag cgaccgctct gttcgagcat
    60121 gtagatagtt acctggtctg gcgtgttttt gccgctctga taacgcgcca tatgactgtt
    60181 cacgctaata cgcatactgt ccagcaccgg cgaattaaga agcaccagta tcaccaacac
    60241 caataacgaa accgcaaggt tcactttgcc ctgaagaaca aggggatttt gcccattacg
    60301 ccagacgata ctgacaaaat accccagcga ccacaccagt aacaccagct ccgccagcgc
    60361 gccctgcaag cggtcgacag tccagccgta ctgagcgacc cgcagccata acccccaggt
    60421 ggcgacgaac acatacagcg gagcaaccag caaagcggtt ttaatcaggc aacgcaacgg
    60481 ccccgtccag ggaagtgacg ctttttgcgg atcgcggaca atagccatta agatcaattg
    60541 caaaaaggcc agcgttaaca gcaacccggc ggcggagatg tggcgagaaa tcgcgctcag
    60601 gcccgtaaac ggcagggtga tgataaacat cggggttagc aatgatacta acggcagcaa
    60661 ccccgtggcg attagcgtga acaacttttg aatagagtcg attaaacgtg actgtgtacg
    60721 cgcgaggatc accgccagtg cggtaaccag accaaatgta agatacataa accagtcggt
    60781 tgcaaaaaag agcgtattga aaaacgtgat gccaacaagt ttaaacaact caccccacag
    60841 taaaagcacc agccacgtca ggccattgga gagaaaaatc accagtaata taagtacgtt
    60901 atgccatact gactggtaaa agtagctata gcggctactg ccattgcgga tacgaaggct
    60961 ttgttgtatc cacggtagca acaacattgc catcaacagc agatagcagc caaaatccca
    61021 aatcgccttt tcagctctcc aggggttcat gccatcagtt tgccacttca gccaaccgct
    61081 catccctgtc gtggcaataa acaccagcgc cagccatccc caaaggcgct tctgtttaaa
    61141 agagatcacg gagaagagta aaacggatga aaacgcgacg gtcgcaggca cgccataaac
    61201 aatccaactg tgattttttc cggcaagcca ggcgataagc aggtagcaaa ccagcccctg
    61261 aagtaatccg gtagcaatca tcccccagcg tgttgccggt gaaagttcaa cgttatccat
    61321 gttggttatc cagaatcaaa aggtgggtta attatcgcat ccgggcagta gtattttgct
    61381 tttttcagaa aataatcaaa aaaagttagc gtggtgaatc gatactttac cggttgaatt
    61441 tgcatcaatt tcattcagga atgcgattcc actcacaata ttcccgccat ataaaccaag
    61501 atttaacctt ttgagaacat tttccacacc taaaatgcta tttctgcgat aatagcaacc
    61561 gtttcgtgac aggaatcacg gagttttttg tcaaatatga atttctccag atacgtaaat
    61621 ctatgagcct tgtcgcggtt aacaccccca aaaagacttt actattcagg caatacatat
    61681 tggctaagga gcagtgaaat gcgcgttaac aatggtttgc ccccgcaaga actcgaggct
    61741 tatggtatca gtgacgtaca tgatatcgtt tacaacccaa gctacgacct gctgtatcag
    61801 gaagagctcg atccgagcct gacaggttat gagcgcgggg tgttaactaa tctgggtgcc
    61861 gttgccgtcg ataccgggat cttcaccggt cgttcaccaa aagataagta tatcgtccgt
    61921 gacgatacca ctcgcgatac tttctggtgg gcagacaaag gcaaaggtaa gaacgacaac
    61981 aaacctctct ctccggaaac ctggcagcat ctgaaaggcc tggtgaccag gcagctttcc
    62041 ggcaaacgtc tgttcgttgt cgacgctttc tgtggtgcga acccggatac tcgtctttcc
    62101 gtccgtttca tcaccgaagt ggcctggcag gcgcattttg tcaaaaacat gtttattcgc
    62161 ccgagcgatg aagagctggc aggtttcaaa ccagacttta tcgttatgaa cggcgcgaag
    62221 tgcactaacc cgcagtggaa agaacagggt ctgaactccg aaaacttcgt ggcgtttaac
    62281 ctgaccgagc gcatgcagct gattggcggc acctggtacg gcggcgaaat gaagaaaggg
    62341 atgttctcga tgatgaacta cctgctgccg ctgaaaggta tcgcttctat gcactgctcc
    62401 gccaacgttg gcgagaaagg cgatgttgcg gtgttcttcg gcctttccgg caccggtaaa
    62461 accacccttt ccaccgaccc gaaacgccgc ctgattggcg atgacgaaca cggctgggac
    62521 gatgacggcg tgtttaattt cgaaggcggc tgctacgcaa aaactatcaa gctgtcgaaa
    62581 gaagcggaac ctgaaatcta caacgctatc cgtcgtgatg cgctgctgga aaacgtcacc
    62641 gtgcgtgaag atggcactat cgactttgat gatggttcaa aaaccgagaa cacccgcgtt
    62701 tcttatccaa tctatcacat cgataacatc gttaagccgg tttccaaagc gggccacgcg
    62761 actaaggtta tcttcctgac tgctgatgct ttcggcgtgt tgccgccggt ttctcgcctg
    62821 actgccgatc aaacccagta tcacttcctc tctggcttca ccgccaaact ggccggtact
    62881 gagcgtggca tcaccgaacc gacgccaacc ttctccgctt gcttcggcgc ggcgttcctg
    62941 tcgctgcacc cgactcagta cgcagaagtg ctggtgaaac gtatgcaggc ggcgggcgcg
    63001 caggcttatc tggttaacac tggctggaac ggcactggca aacgtatctc gattaaagat
    63061 acccgcgcca ttatcgacgc catcctcaac ggttcgctgg ataatgcaga aaccttcact
    63121 ctgccgatgt ttaacctggc gatcccaacc gaactgccgg gcgtagacac gaagattctc
    63181 gatccgcgta acacctacgc ttctccggaa cagtggcagg aaaaagccga aaccctggcg
    63241 aaactgttta tcgacaactt cgataaatac accgacaccc ctgcgggtgc cgcgctggta
    63301 gcggctggtc cgaaactgta atgatttgaa gctggagaat atctatccag tatcttatag
    63361 aaagcaaaac gggaggcacc ttcgcctccc gtttatttac ccttcttttg tcgtgccctg
    63421 cgcccgcgtt accggcactg gcagccaggc gcgaatggaa agcccgcccc gctcgctggt
    63481 gccaagctcc agcatcccgt tatggttatc cacgatacgc tgcacaattg ccagccctaa
    63541 tcccgtgccg ctaatggtgc gcgcactgtc gccgcggaca aacggctgga acaggtgctt
    63601 acgttgttcc ggcgcaattc ccggaccgtc atcttccacc tggaaccagg cgcgattcgg
    63661 ctccgttccg ctgctgactt tgatccagcc attgccataa cgggcggcgt tgaccaccat
    63721 attcgccacc gcgcgtttga tcgacagcgg gtgcattttc acttcaatgc tgccggggta
    63781 aagcgcggtt tcaatttccc gctcatagcc actttcggca gcaatcacct caccgagcac
    63841 tgcattaaga tccgccattt ccatcggcat ctcctgcccg gtgcgcaggt agtcgataaa
    63901 ctgctcaatg atggcgttgc actcttcgat atctttattg atcgattctg ccagatagcc
    63961 atcctgctcg ctcatcatct cagtcgccag gcgaatacgc gtcagcggcg tgcgcaagtc
    64021 gtgacttacc cccgccatca gcagcgtgcg gtcatccgcc agttgcttaa caccagccgc
    64081 catatggtta aaggcacggg taacggaacg cacctccgaa gcgccatatt cacgcagcgg
    64141 cggcggaata atccctttac caacctgcaa ggctgcgtgt tcgagatcga ccaacggtcg
    64201 gttctggata cgaataaaca gccacgcccc gcctatcgcc aatagcataa tcgccagcgt
    64261 atagcggaac agcggagaga aatcgccctg atgaatttcg gtcagcggca cgcgtaccca
    64321 gatattgggc gacagccagg ttttcagcca gacgacaggc gaacttttgt tgacctcaac
    64381 gcgcacttcc gtcgggccgc ccagttgctg tgccatctga tggcttaaga attcatagtg
    64441 ttgcgcccaa cgcagacctg cctcttcggc agcctcgttg gagtagagag agatccccag
    64501 ctcacggtag atctcccgac ggaaagcggg aggcacaacc aactgcgtgc cgtcctccag
    64561 ttgcagtttg tcggtcatca acatacgcac ttcgtacgcg aggactttat taaactgctg
    64621 gaggctcggc aaaatcgcga agttcagcac caccagataa gtcgtcacca ggctggcgaa
    64681 cagcaaggtg acgatgagca ataacgtacg ggcaaatgaa cttcgtggcg agaagcgcaa
    64741 tcgcctcatg ctttagagcc gtccggtaca aagacgtagc ccaggcccca aacggtctga
    64801 atgtaacgcg gatgcgctgg atcttcttct accatgcggc gcagacgcga aatctgcacg
    64861 tcgatggagc gttccattgc ggaatattca cgaccacggg caaggttcat cagcttatcg
    64921 cgggagagcg gctcacgcgg atggctgacc agtgccttca gtaccgcaaa ctcaccgctg
    64981 gtgagcggca tcggctcatc ttcgcggaac atttcgcgcg taccgaggtt aagtttgaac
    65041 ttaccgaaag caattaccgc ctcttcctgt gacggtgcgc ctggcagttc gttcgcctga
    65101 cgacgcagca ccgcacgaat acgggccagc agttcacgcg ggttaaacgg ttttggaatg
    65161 tagtcgtcag cgccaatctc caggcctacg atacggtcca cttcttcccc tttcgccgtc
    65221 accataatga tcggcatcgg gttgctctga ctacgaagac gtcggcaaat cgacaagcca
    65281 tcttcaccag gtaacattaa atccagtacc ataagatgga aagattcacg agtcagcagg
    65341 cgatccatct gttctgcatt agcgacgctt cgaacctgga agccttgttc ggtgagataa
    65401 cgttccagca gcgcacgcag gcgcatgtcg tcatcgacca ccagaatctt gtagttctct
    65461 tgcattgttt gtactcccaa aggttcgcaa caatttgtaa gcgtgtattc ttaaaaaagc
    65521 tcacgttcgt caccagctaa atctggtata aatttcagcc taaattgtta caaagcatat
    65581 taaacagcag cttaagtata caatttattc ggcgaaacat tattgattct gttgatatga
    65641 tcacgttata cccaatgtgc gcattatcaa acagacaaag ggaatcaacg agatgaaaac
    65701 gcccctggtt acccgggaag ggtatgaaaa actcaaacaa gagcttaatt atctctggcg
    65761 tgaagaacgc ccggaggtca caaaaaaggt gacctgggcc gcaagtctgg gcgaccgcag
    65821 cgaaaatgct gactatcagt ataataaaaa gcgtctgcgt gaaatcgacc gtcgcgtgcg
    65881 ctatctcact aaatgcctgg aaaatctcaa aatcgtcgat tactcccctc agcaggaagg
    65941 caaagtattt tttggcgcgt gggtggagat tgaaaacgac gatggcgtga ctcaccgttt
    66001 ccgtattgtc ggctacgatg aaatttttgg ccgtaaagat tacatctcta tcgattcccc
    66061 gatggcccgc gcattgctga aaaaagaagt cggcgatctg gcggtggtga atacccctgc
    66121 cggggaagcg aactggtatg ttaatgctat cgagtacgtg aaaccgtaag gaagagtctt
    66181 aacctcctgc cgatggctgg catttttgcc agccagtccg tataactatc ccctgatttt
    66241 tgatccgaaa agatgaactc aaaccatgat gaatgattcg ttctgccgca ttattgcggg
    66301 tgaaattcag gcgcgcccgg aacaggttga cgctgccgtt cgcctgcttg acgaagggaa
    66361 taccgtgccg tttatcgcac gttatcgtaa ggaaatcacc ggcggtctgg atgacacgca
    66421 gctgcgtaat ctggaaacgc gtctgagcta tctgcgcgag ctggaagaga gacgtcaggc
    66481 gatcctcaaa tccatttccg agcaaggcaa actcaccgat gatctggcga aggccatcaa
    66541 cgccacccta agcaaaaccg aactcgaaga cctctacctg ccctacaaac ctaaacgccg
    66601 cacccgcggg caaatcgcca ttgaagcagg gcttgagccg ttggctgacc tgctgtggag
    66661 cgatccgtca cacacgccag aagtcgccgc tgcacaatat gttgatgccg ataaaggcgt
    66721 ggcagatacc aaagccgcgc tggatggcgc gcgctatatc ctgatggaac ggtttgccga
    66781 agatgccgcg ctgctggcga aagtgcgtga ttatctgtgg aagaacgcgc atttggtttc
    66841 tacggtggtg agcggtaaag aagaggaagg ggcgaaattc cgcgactatt tcgatcatca
    66901 cgaaccgttg tccacggtgc cttctcaccg cgcgctggcg atgttccgtg ggcgtaacga
    66961 aggcgtactc cagctttcgc tgaatgccga tccgcagttc gatgagccgc ccaaagagag
    67021 ctattgcgag caaatcatca tggatcacct tggcctgcgc ctgaacaatg ccccggcgga
    67081 tagctggcgc aaaagcgtag tgagctggac ctggcgcatc aaggtgctga tgcatctgga
    67141 aaccgaactg atgggtaccg tgcgcgaacg cgcggaagat gaagcaatca acgtctttgc
    67201 ccgtaacctg cacgatctgc tgatggcggc ccctgccgga ctgcgtgcaa cgatgggcct
    67261 cgatccgggt ctgcgtaccg gggtaaaagt ggcggtggtc gatgccactg gcaaactggt
    67321 ggcgaccgat accatttacc cgcacaccgg acaagccgca aaagcagcga tgaccgttgc
    67381 tgccttgtgt gaaaaacata acgttgaact ggtggcgatc ggcaacggta cagcttcccg
    67441 cgaaactgaa cgtttctatc tcgacgtgca gaagcagttc ccgaaagtga ccgcacagaa
    67501 agtgatcgtc agcgaagctg gcgcgtcggt ttactcagct tccgagctgg cagcgcagga
    67561 gttcccggat ctcgacgttt cgctgcgtgg tgcggtatct atcgcccgcc gtttgcagga
    67621 tccgctggcg gagctggtga aaatcgatcc gaaatctatc ggcgtaggtc agtatcagca
    67681 tgacgtcagc cagacgcaac tggcccgcaa actggatgca gtagtagaag actgcgtaaa
    67741 cgccgttggc gtcgatctca acaccgcttc tgttccgcta ttaacccgcg tggcgggcct
    67801 gacgcgcatg atggcgcaaa acatcgtggc ctggcgcgat gagaacggcc agttccagaa
    67861 ccgtcagcaa ctgctgaaag tcagccgtct ggggccgaaa gccttcgagc agtgcgcggg
    67921 cttcctgcgc attaaccacg gtgataaccc gctggacgcc tctaccgttc atccggaagc
    67981 ctatccggtg gtggaacgca ttctggcagc aacacagcag gcactgaaag atctgatggg
    68041 taacagcagc gaactgcgta acctgaaagc gtctgacttt actgatgaaa aattcggtgt
    68101 gccgacagta actgacatca tcaaagagct ggaaaaaccg ggtcgcgatc cgcgtccgga
    68161 atttaaaacc gctcagtttg ccgatggcgt cgagacaatg aacgacctgc aaccgggtat
    68221 gatcctcgaa ggcgcagtga ccaacgtcac caactttggc gcgtttgtcg atattggcgt
    68281 gcatcaggac ggcctggttc acatctcttc attatcgaac aagtttgtgg aagatccgca
    68341 taccgtggtg aaagcgggcg acattgtgaa ggtgaaagtg ctggaagtgg atcttcagcg
    68401 taaacgtatc gccctgacta tgcgtctgga cgagcagcct ggcgaaacca actctcgtcg
    68461 cggcggcggt aatgaacgcc cgcaaaacaa ccgcccggca gccaaaccac gcggtcgtga
    68521 agcgcagcct gccggtaata gcgcgatgat ggatgcgctg gcggcagcga tgggtaaaaa
    68581 acgttaaacg cccggactgg cccctaaacc tgcgcgataa ggcgttcacg ccgcatctgg
    68641 caacgaacgc tttgcctgac ataaaagtgc cggagaatat ctccggcact tttattccac
    68701 agccaaactc acaatatact ccgttaatat ttatcacttc attaacaact gaaaccttaa
    68761 ttaaacatta gccagtccgg gtaactcact attcgaatta tattttcgct gcgatataac
    68821 cttgagccac atcaacattg agtcagatta ttattcaaac caacattcgc acacatttta
    68881 agtattgctg atagaaacca ttctcattat cattgtgttg ttgattattt aatctctcct
    68941 ttgttggcaa atcatctggt ctcatgtcgc ggtcaaacgc cccatgaggt agttatccag
    69001 ttaatgagaa acaagtaggc acctatgcaa tacactccag atactgcgtg gaaaatcact
    69061 ggcttttccc gtgaaatcag cccggcatat cgccaaaaac tgctttctct aggcatgtta
    69121 cctggctcct cttttgatgt ggtgcgcgtc gctccactcg gcgaccccat tcatatcgaa
    69181 acccgtcgtg tgagcctggt attacgcaaa aaagatctgg ccttattaga agtggaagcg
    69241 gtttcctgtt aatacggtga taacaacaat gaaaaaatta accattggct taattggtaa
    69301 tccaaattct ggcaagacaa ccttatttaa ccagctcact ggcgcacgtc agcgtgtagg
    69361 taactgggct ggcgttaccg tcgaacgtaa agaagggcaa ttctccacca ccgatcatca
    69421 ggtcacgctg gtggacctgc ccggcaccta ttctctgacc accatctcat cgcagacctc
    69481 gctcgatgag caaatcgcct gtcactacat tttgagtggc gacgccgacc tgctgattaa
    69541 cgtggtggat gcgtctaacc ttgagcgtaa cctgtacctg acgctgcaac tgctggaact
    69601 cggcattccc tgcatcgtgg cgctgaacat gctcgacatt gccgagaagc aaaatattcg
    69661 tattgaaatt gatgctctat cggcacgtct tggctgcccg gtgatcccgc tggtttcaac
    69721 ccgtggtcgc ggtattgaag cgctcaagct ggcgattgat cgctataaag ctaacgagaa
    69781 tgtggaactg gtgcattacg cacagccgct actcaacgaa gcagattcac tggcaaaagt
    69841 gatgccttcc gacatcccgc tgaaacaacg tcgctggctg ggcctgcaaa tgctggaagg
    69901 cgatatctac agccgcgcct acgccggtga agcgtcgcag catctggatg ccaccctcgc
    69961 ccgtctgcgt aatgaaatgg atgatccggc gctgcacatt gccgatgcgc gttaccagtg
    70021 cattgctgcc atctgtgatg tggtaagcaa caccctgacg gcagaaccca gccgtttcac
    70081 cactgcggta gataaaatcg tgctcaaccg tttcctcggt ctgccgattt tcctctttgt
    70141 gatgtacctg atgttcctgc tggctatcaa catcggcggg gcgttacagc cgctgtttaa
    70201 cgtcggctcc gtggcgctat ttgtgcatgg tattcaatgg attggctaca cgctccactt
    70261 cccggactgg ctgactatct tcctcgccca gggcctgggt ggcggtatta acaccgtgct
    70321 gccactggtg ccgcagatcg gcatgatgta cctgttcctc tccttccttg aggactccgg
    70381 ctatatggcg cgtgcagcgt ttgtgatgga ccgcctgatg caggcgctgg gcttgccggg
    70441 gaaatccttt gtgccgctga tcgtcggttt cggttgtaac gtaccgtcgg taatgggcgc
    70501 acgtacgctt gatgcaccgc gtgaacgtct gatgaccatc atgatggcac cgtttatgtc
    70561 ctgcggcgcg cgtctggcta tcttcgcagt attcgcggct gccttcttcg ggcagaacgg
    70621 tgccctggcg gttttctcgc tgtatatgct cggtatcgtg atggcggtgc tgactggcct
    70681 gatgctcaag tacaccatca tgcgcggtga agcgacgccg tttgtcatgg agctgccggt
    70741 ctaccatgta ccacacgtta aaagcctgat tatccagacc tggcagcgtc tgaagggctt
    70801 cgttctgcgt gctggtaaag tgattatcat cgtcagcatt ttcctgagcg ctttcaacag
    70861 cttctcgttg agcgggaaaa tcgtcgataa catcaacgac tcggcgctgg cttccgtcag
    70921 ccgggtgata acccctgtct tcaagccgat tggcgtacat gaagataact ggcaggcaac
    70981 ggttggcctg tttacgggcg caatggcaaa agaagtggtg gtgggtacgc tcaacaccct
    71041 ctacaccgca gaaaatattc aggacgaaga gttcaatccg gcggaattca acctcggtga
    71101 agagctgttc agtgcggtag atgaaacctg gcagagcctg aaagacacct tcagccttag
    71161 cgtactgatg aaccccattg aagctagcaa aggcaacggt gaaatgggta ccggggcgat
    71221 gggcgtgatg gatcagaaat tcggtagcgc agctgccgca tacagctacc tgattttcgt
    71281 cctgctgtat gtaccatgta tctcggtgat gggagccatc gcccgtgaat caagccgtgg
    71341 ctggatgggc ttctccatcc tgtgggggct gaatatcgct tactcactgg caacattgtt
    71401 ctatcaggtc gccagctaca gtcagcaccc aacttacagc ctggtgtgca ttctggcggt
    71461 aatcctgttt aacatcgtag ctatcggtct gctgcgccgc gcgcgtagcc gggtggatat
    71521 cgaactgctg gcaacccgca agtcggtaag cagttgctgc gcagccagca ccaccggtga
    71581 ttgccattaa tggcttcact tattcaggta cgtgatttgc tggcgttacg gggccgtatg
    71641 gaagcgaccc agataagcca gacattgaac actccgcagc caatgattaa cgccatgctg
    71701 caacagctgg aaagcatggg caaagccgtg cggattcagg aagaacctga cggctgcctc
    71761 tctggcagtt gtaaaagctg cccggaaggc aaagcctgtc tgcgcgagtg gtgggcgctg
    71821 cgttaaccat actccatcgc ctgttttgaa aagcccggta tgcgtttgca tccgggcttt
    71881 tttgcgtgcg gctttccata aaaatgcaat tcttgcagcg caacgtaagt tcctctgaaa
    71941 gcgtctcgca gggatgaaaa ctcgctaatg cacagatgtg gagtggcgcg tagagtcgcg
    72001 gcattcaaac aacaggtgaa ggaacgccat gagtaaaaag cagagttcca ccccacatga
    72061 tgcgctgttc aaactctttt tacgccaacc ggagacggca cgcgattttc ttgcgtttca
    72121 tttaccggca cccattcacg cgctctgtga tatgaaaacc ctcaagctgg agtcgaggag
    72181 ctttattgat gacgatctgc gtgaaagcta ttccgatgtg ctgtggtcgg tgaaaacgga
    72241 acaaggacca ggatacatct attgtctgat tgaacatcaa agcacctcaa acaaactgat
    72301 cgcatttcgc atgatgcgtt acgctattgc cgcaatgcaa aatcaccttg atgctggata
    72361 caaaacgttg ccgatggtgg tgccattgtt gttttaccac ggtattgaaa gcccctatcc
    72421 ctattcgctg tgttggctgg attgtttcgc cgatcccaaa ctggcaaggc agctttatgc
    72481 ctccgcattt ccgctgattg atgtcaccgt catgcctgat gatgaaatca tgcagcaccg
    72541 acgtatggcg ctgctggagt taattcaaaa acatattcgt caacgcgatc tgatggggct
    72601 ggtagagcaa atggcctgct tattaagtag tggatacgct aatgacagac aaatcaaagg
    72661 gctgtttaat tacatactgc aaactggcga cgctgtacgt tttaacgatt ttatcgacgg
    72721 cgttgccgaa cgttcaccga aacacaagga gagtttaatg actattgcgg aaagattgcg
    72781 gcaggagggg gaacaatcca aagccctgca tatagccaaa ataatgcttg aatccggagt
    72841 ccctcttgca gacatcatgc gatttaccgg gctgtcagaa gaagagttgg ctgcggcgag
    72901 tagactggcc ccctgaatct ccaga
//
LOCUS       DAJTQY010000006        71728 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.006, whole genome shotgun sequence.
ACCESSION   DAJTQY010000006 DAJTQY010000000
VERSION     DAJTQY010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 71728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 71728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..71728
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.006"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(1337..2932)
                     /locus_tag="OO349_000395"
     CDS             complement(1337..2932)
                     /locus_tag="OO349_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate permease LctP family transporter"
                     /protein_id="HCR5865597.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSLVISITDDQRLQVLLIGFSFGALLEGAAGFGATVAFGALGVPILVAGQVTGIDPF
                     HIGAMAGRQLPFLSVLVPFWLVAMMDGWKGVKETWPAALVAGGSFAVTQFFTSNYIGP
                     ELPDITSALVSIVSLALFLKVWRPKNTETAISMGQSAGAMVVNKPSSGGPVPSEYSLG
                     QIIRAWSPFLILTVLVTIWTMKPFKALFAPGGAFYSLVINFQIPHLHQQVLKAAPIVA
                     QPTPMDAVFKFDPLSAGGTAIFIAAIISIFILGVGIKKGIGVFAEMLISLKWPILSIG
                     MVLAFAFVTNYSGMSTTLALVLAGTGVMFPFFSPFLGWLGVFLTGSDTSSNALFGSLQ
                     STTAQQINVSDTLLVAANTSGGVTGKMISPQSIAVACAATGMVGRESELFRYTVKHSL
                     IFASVIGVITLLQAYVFTGMLVS"
     gene            complement(3287..5458)
                     /gene="glcB"
                     /locus_tag="OO349_000396"
     CDS             complement(3287..5458)
                     /gene="glcB"
                     /locus_tag="OO349_000396"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HCR5865598.1"
                     /translation="MSQTITQGRLRIDANFKRFVDGEVLPGVELDAAAFWHNVDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHHTNVQSVQANIAQTEFS
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            complement(5480..5884)
                     /locus_tag="OO349_000397"
     CDS             complement(5480..5884)
                     /locus_tag="OO349_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HCR5865599.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKVAAAVLAK"
     gene            complement(5889..7108)
                     /gene="glcF"
                     /locus_tag="OO349_000398"
                     /pseudo
     CDS             complement(5889..7108)
                     /gene="glcF"
                     /locus_tag="OO349_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
     gene            complement(7119..8171)
                     /gene="glcE"
                     /locus_tag="OO349_000399"
     CDS             complement(7119..8171)
                     /gene="glcE"
                     /locus_tag="OO349_000399"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HCR5865600.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGTGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMSEIAEWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTADDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            complement(8171..9670)
                     /gene="glcD"
                     /locus_tag="OO349_000400"
                     /pseudo
     CDS             complement(8171..9670)
                     /gene="glcD"
                     /locus_tag="OO349_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
     gene            9921..10698
                     /gene="glcC"
                     /locus_tag="OO349_000401"
                     /pseudo
     CDS             9921..10698
                     /gene="glcC"
                     /locus_tag="OO349_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708791.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
     gene            complement(10822..11963)
                     /locus_tag="OO349_000402"
                     /pseudo
     CDS             complement(10822..11963)
                     /locus_tag="OO349_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
     gene            12325..14055
                     /locus_tag="OO349_000403"
     CDS             12325..14055
                     /locus_tag="OO349_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HCR5865601.1"
                     /translation="MVYMSNKIFTHSLPMRYADFPTLVDALDYAALSRAGMNFYDRRC
                     QLEDQLEYQTLKTRAEAGAKRLLSLNLKKGERVALIAETSSGFVEAFFACQYAGLVAV
                     PLAIPMGVGQRDSWSAKLQGLLASCQPAAIITGDEWLPLVNAATHNNPELHVLSHAWF
                     KALPEADVALQRPVPNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPS
                     DRCVSWQPFYHDMGLVGFLLPPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVA
                     PPFGYELCQRRVNEKDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDNKTFM
                     PCYGLAENALAVSFSDEASGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALP
                     EHGIEIRNEAGMPVAERVVGHICISGPSLMSGYFGDQVSQDEIAATGWLDTGDLGYLL
                     DGYLYVTGRIKDLIIIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCR
                     ISDEERRGQLIHALAARIQSEFGVTAAIDLLPPHSIPRTSSGKPARAEAKKRYQKAYA
                     ASLNVQESLA"
     gene            14052..14966
                     /locus_tag="OO349_000404"
     CDS             14052..14966
                     /locus_tag="OO349_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HCR5865602.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSELVAGASVVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYAKSKYVAEQRLAAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDW
                     QRVRQLAAKARCGSVRMVDIPLPVLTGLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            14999..15247
                     /locus_tag="OO349_000405"
     CDS             14999..15247
                     /locus_tag="OO349_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR5865603.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            15247..16418
                     /locus_tag="OO349_000406"
                     /pseudo
     CDS             15247..16418
                     /locus_tag="OO349_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
     gene            complement(16454..17533)
                     /gene="lptG"
                     /locus_tag="OO349_000407"
     CDS             complement(16454..17533)
                     /gene="lptG"
                     /locus_tag="OO349_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HCR5865604.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDGVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIEDDKTWVLHGVNHKKWLNGKETL
                     ETLDNLAWQSAFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYRLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(17530..18600)
                     /gene="lptF"
                     /locus_tag="OO349_000408"
     CDS             complement(17530..18600)
                     /gene="lptF"
                     /locus_tag="OO349_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HCR5865605.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRAGSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLILVARDFSLLQKFSR"
     gene            complement(18631..19191)
                     /locus_tag="OO349_000409"
     CDS             complement(18631..19191)
                     /locus_tag="OO349_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HCR5865606.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            complement(19203..20027)
                     /locus_tag="OO349_000410"
     CDS             complement(19203..20027)
                     /locus_tag="OO349_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5865607.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYYAPLALDSDICQRLELLISRFPL"
     gene            complement(20027..21376)
                     /locus_tag="OO349_000411"
     CDS             complement(20027..21376)
                     /locus_tag="OO349_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HCR5865608.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVESALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVAAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            complement(21422..22180)
                     /locus_tag="OO349_000412"
     CDS             complement(21422..22180)
                     /locus_tag="OO349_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5865609.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERSLYQKMAQYRPELIIRLGIDIDTAISRKPDHEYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQVQKAVSLVAIVSDRRSLT"
     gene            complement(22212..22925)
                     /locus_tag="OO349_000413"
     CDS             complement(22212..22925)
                     /locus_tag="OO349_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HCR5865610.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPCGCYLRSKAAHVHEKPLTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGYLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     QPADEIMQASLRAIHAALS"
     gene            23099..23791
                     /locus_tag="OO349_000414"
     CDS             23099..23791
                     /locus_tag="OO349_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HCR5865611.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AITDFMTARGYH"
     gene            complement(23840..25339)
                     /gene="pitB"
                     /locus_tag="OO349_000415"
     CDS             complement(23840..25339)
                     /gene="pitB"
                     /locus_tag="OO349_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HCR5865612.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPIDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRFWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(25631..27490)
                     /gene="gss"
                     /locus_tag="OO349_000416"
     CDS             complement(25631..27490)
                     /gene="gss"
                     /locus_tag="OO349_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HCR5865613.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            27695..28561
                     /gene="yghU"
                     /locus_tag="OO349_000417"
     CDS             27695..28561
                     /gene="yghU"
                     /locus_tag="OO349_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HCR5865614.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPFSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETLNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(28684..28932)
                     /gene="hybG"
                     /locus_tag="OO349_000418"
     CDS             complement(28684..28932)
                     /gene="hybG"
                     /locus_tag="OO349_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HCR5865615.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(29066..29407)
                     /gene="hypA"
                     /locus_tag="OO349_000419"
     CDS             complement(29066..29407)
                     /gene="hypA"
                     /locus_tag="OO349_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCR5865616.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(29400..29888)
                     /gene="hybE"
                     /locus_tag="OO349_000420"
     CDS             complement(29400..29888)
                     /gene="hybE"
                     /locus_tag="OO349_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HCR5865617.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRRSE
                     NA"
     gene            complement(29881..30375)
                     /locus_tag="OO349_000421"
     CDS             complement(29881..30375)
                     /locus_tag="OO349_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HCR5865618.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(30375..32078)
                     /gene="hybC"
                     /locus_tag="OO349_000422"
     CDS             complement(30375..32078)
                     /gene="hybC"
                     /locus_tag="OO349_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HCR5865619.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(32075..33253)
                     /gene="hybB"
                     /locus_tag="OO349_000423"
     CDS             complement(32075..33253)
                     /gene="hybB"
                     /locus_tag="OO349_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HCR5865620.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIGLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(33243..34229)
                     /gene="hybA"
                     /locus_tag="OO349_000424"
     CDS             complement(33243..34229)
                     /gene="hybA"
                     /locus_tag="OO349_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HCR5865621.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(34232..35350)
                     /gene="hybO"
                     /locus_tag="OO349_000425"
     CDS             complement(34232..35350)
                     /gene="hybO"
                     /locus_tag="OO349_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HCR5865622.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGSAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEKGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(35539..35826)
                     /locus_tag="OO349_000426"
     CDS             complement(35539..35826)
                     /locus_tag="OO349_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HCR5865623.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYCLEN"
     gene            complement(35945..36832)
                     /locus_tag="OO349_000427"
     CDS             complement(35945..36832)
                     /locus_tag="OO349_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCR5865624.1"
                     /translation="MPRLTAKDFPQELLDYYDYYVHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVG
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQATGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPPYEAALKDNNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            36989..38029
                     /gene="gpr"
                     /locus_tag="OO349_000428"
     CDS             36989..38029
                     /gene="gpr"
                     /locus_tag="OO349_000428"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HCR5865625.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(38069..38563)
                     /locus_tag="OO349_000429"
     CDS             complement(38069..38563)
                     /locus_tag="OO349_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HCR5865626.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLKWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            38754..39638
                     /gene="yghA"
                     /locus_tag="OO349_000430"
     CDS             38754..39638
                     /gene="yghA"
                     /locus_tag="OO349_000430"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCR5865627.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPIPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASVITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(39910..40335)
                     /gene="exbD"
                     /locus_tag="OO349_000431"
     CDS             complement(39910..40335)
                     /gene="exbD"
                     /locus_tag="OO349_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HCR5865628.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(40342..41076)
                     /gene="exbB"
                     /locus_tag="OO349_000432"
     CDS             complement(40342..41076)
                     /gene="exbB"
                     /locus_tag="OO349_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCR5865629.1"
                     /translation="MGNNLMQTDLSVWGMYQYADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            41328..42515
                     /gene="metC"
                     /locus_tag="OO349_000433"
     CDS             41328..42515
                     /gene="metC"
                     /locus_tag="OO349_000433"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HCR5865630.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            42655..43314
                     /locus_tag="OO349_000434"
     CDS             42655..43314
                     /locus_tag="OO349_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5865631.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGSWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(43354..44253)
                     /gene="yqhC"
                     /locus_tag="OO349_000435"
     CDS             complement(43354..44253)
                     /gene="yqhC"
                     /locus_tag="OO349_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HCR5865632.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            44447..45610
                     /gene="yqhD"
                     /locus_tag="OO349_000436"
     CDS             44447..45610
                     /gene="yqhD"
                     /locus_tag="OO349_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HCR5865633.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGTGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            45715..46542
                     /gene="dkgA"
                     /locus_tag="OO349_000437"
     CDS             45715..46542
                     /gene="dkgA"
                     /locus_tag="OO349_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HCR5865634.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGKIAKLDQGKRLGPDPDQFGG"
     gene            46742..47668
                     /locus_tag="OO349_000438"
     CDS             46742..47668
                     /locus_tag="OO349_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HCR5865635.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     VDHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPESFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            47719..47976
                     /gene="yqhH"
                     /locus_tag="OO349_000439"
     CDS             47719..47976
                     /gene="yqhH"
                     /locus_tag="OO349_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HCR5865636.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(48019..50238)
                     /locus_tag="OO349_000440"
     CDS             complement(48019..50238)
                     /locus_tag="OO349_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708831.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HCR5865637.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVVHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIAPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VIVQPPRQKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKKPLAKIGYK
                     SEGVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(50349..51761)
                     /gene="ftsP"
                     /locus_tag="OO349_000441"
     CDS             complement(50349..51761)
                     /gene="ftsP"
                     /locus_tag="OO349_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HCR5865638.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVS"
     gene            complement(51836..52573)
                     /gene="plsC"
                     /locus_tag="OO349_000442"
                     /gene_synonym="parF"
     CDS             complement(51836..52573)
                     /gene="plsC"
                     /locus_tag="OO349_000442"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HCR5865639.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAENYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(52807..55065)
                     /gene="parC"
                     /locus_tag="OO349_000443"
     CDS             complement(52807..55065)
                     /gene="parC"
                     /locus_tag="OO349_000443"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HCR5865640.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(55611..56093)
                     /locus_tag="OO349_000444"
     CDS             complement(55611..56093)
                     /locus_tag="OO349_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HCR5865641.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDAPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(56146..56538)
                     /gene="ygiW"
                     /locus_tag="OO349_000445"
     CDS             complement(56146..56538)
                     /gene="ygiW"
                     /locus_tag="OO349_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HCR5865642.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSVTQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            56690..57349
                     /gene="qseB"
                     /locus_tag="OO349_000446"
     CDS             56690..57349
                     /gene="qseB"
                     /locus_tag="OO349_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HCR5865643.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLNLTARDALAERVEGLRLGADDYLCKP
                     FALIEVAAKLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            57346..58620
                     /gene="qseC"
                     /locus_tag="OO349_000447"
     CDS             57346..58620
                     /gene="qseC"
                     /locus_tag="OO349_000447"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HCR5865644.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNGPGVTPEALARIGERFYRPPGQTATGSGL
                     GLSIVQRIAKLHDMNVEFGNAEQGGFEAKVSW"
     gene            complement(58666..58998)
                     /locus_tag="OO349_000448"
     CDS             complement(58666..58998)
                     /locus_tag="OO349_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HCR5865645.1"
                     /translation="MLKQKIKTIFEALLYIMLTYWLIDSFFAFNKYDWMLESGGNICS
                     IPSVSGEDRILQAMIAAFFLLTPLIILILRKLFMREMFEFWLYVFSLVICLVCGWWLF
                     WGRFIFCY"
     gene            59317..59898
                     /gene="mdaB"
                     /locus_tag="OO349_000449"
     CDS             59317..59898
                     /gene="mdaB"
                     /locus_tag="OO349_000449"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HCR5865646.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYNVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGSLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            59929..60243
                     /locus_tag="OO349_000450"
     CDS             59929..60243
                     /locus_tag="OO349_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCR5865647.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(60291..62183)
                     /gene="parE"
                     /locus_tag="OO349_000451"
     CDS             complement(60291..62183)
                     /gene="parE"
                     /locus_tag="OO349_000451"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HCR5865648.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQGLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(62212..62793)
                     /gene="yqiA"
                     /locus_tag="OO349_000452"
     CDS             complement(62212..62793)
                     /gene="yqiA"
                     /locus_tag="OO349_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HCR5865649.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(62793..63620)
                     /gene="cpdA"
                     /locus_tag="OO349_000453"
     CDS             complement(62793..63620)
                     /gene="cpdA"
                     /locus_tag="OO349_000453"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HCR5865650.1"
                     /translation="MESLLTLPLAGEARGRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(63645..64067)
                     /locus_tag="OO349_000454"
     CDS             complement(63645..64067)
                     /locus_tag="OO349_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HCR5865651.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(64068..64697)
                     /gene="nudF"
                     /locus_tag="OO349_000455"
     CDS             complement(64068..64697)
                     /gene="nudF"
                     /locus_tag="OO349_000455"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HCR5865652.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            64902..66383
                     /gene="tolC"
                     /locus_tag="OO349_000456"
     CDS             64902..66383
                     /gene="tolC"
                     /locus_tag="OO349_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HCR5865653.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLEREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            66531..67202
                     /locus_tag="OO349_000457"
     CDS             66531..67202
                     /locus_tag="OO349_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HCR5865654.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            67208..68368
                     /locus_tag="OO349_000458"
     CDS             67208..68368
                     /locus_tag="OO349_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCR5865655.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLSEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDEIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(68406..69194)
                     /gene="ygiD"
                     /locus_tag="OO349_000459"
     CDS             complement(68406..69194)
                     /gene="ygiD"
                     /locus_tag="OO349_000459"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HCR5865656.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQRLVELLAPIPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPVAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGVWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            69337..70110
                     /gene="zupT"
                     /locus_tag="OO349_000460"
     CDS             69337..70110
                     /gene="zupT"
                     /locus_tag="OO349_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HCR5865657.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQLLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(70168..70338)
                     /locus_tag="OO349_000461"
     CDS             complement(70168..70338)
                     /locus_tag="OO349_000461"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5865658.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(70599..71252)
                     /gene="ribB"
                     /locus_tag="OO349_000462"
     CDS             complement(70599..71252)
                     /gene="ribB"
                     /locus_tag="OO349_000462"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HCR5865659.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(71356..71504)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            71626..>71728
                     /gene="ubiK"
                     /locus_tag="OO349_000463"
     CDS             71626..>71728
                     /gene="ubiK"
                     /locus_tag="OO349_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HCR5865660.1"
                     /translation="MIDPKKIEQIARQVHESMPKGVREFGEDVEKKIR"
BASE COUNT        17914 a        19087 c        17685 g        17042 t
ORIGIN      
        1 atttacgtac cgtttaaaca aagacaatac gtctctttat ttctcataaa accccaatcc
       61 cttacagggc ttattgaaaa atttaaaaat cacaaatttc tcaatactcg aaatgagatc
      121 acataagaaa tcaaaaagcc ttctcatcta ttttaagttt tcccaacgga aaataagagc
      181 tttcattaat actgaaccac tttactgttc tatttgttca ggtattgcat tacggtgtgc
      241 tgagatccat cggtgcatcc ctgtatcccg cgatgagact aaaatagtcg tgatgtaagc
      301 tcgtatcaga ttcttagtga ataccacatt tgttataatt aagtgaaaag atacattgct
      361 gatttacatt atttggttta caaaatgtca tcacttgcag agttatcgct gacagatacc
      421 tttaatctct tactactttc gtgttattgc aatacaacca tccgtaaagc agggctattt
      481 ttagcaggaa atactcaggt gtcaacattc gaactccaac tctgaaatat tcatataagt
      541 taatgatttt aatgatttta atgattttaa tgattttaat gattttttca tttattcaca
      601 cctaataaaa gtattcaaat tcagatgatt tttctgctca acagacacaa acaggcaata
      661 atactctcca aagcgaacaa tatttggcat tacgatttaa tgcttcataa tcaccaaatt
      721 atttaaccat aaattttctt atttctaaca aaatgattgg tgtgttgttg gagttaaaat
      781 acatacataa tattaacatc tgccaatatt tcactttccg caagacggat gatttcgaat
      841 cctcatccac gttaccctat cccaaatatc tttattaata ggcagtaacg aacactgtta
      901 tagcaagatt aacttattaa cctaacggca tcttttatgt aacaagtgga gataaattgc
      961 ttaaaattga tcctcgacaa atcttaaaag ttctcattat tctgcaaaat cagctttgcg
     1021 aataatctta tgtttttggg agttttggga attttgatct actcattgtt ctagtgaaaa
     1081 atataaataa gagaaaaaac gccattcgtc atgaaagcaa cagatgaagc aatgtaataa
     1141 catactaaaa ttgatctcat gaaagtgggt actttataac cagcacagaa gccaagttgt
     1201 ataaatatta aacaattcgc atcgtaaaac ggagactacg tataaagaag ccaatcgtaa
     1261 aaacggttcc ctggcgagat aaacgacaga caaatggaaa gccgggtgat atttaccttc
     1321 atccggcttt attgatttac gagactaaca tcccggtaaa cacatacgcc tgcagcaggg
     1381 tgataacgcc gataacgctg gcaaaaatca gactgtgctt cacggtgtag cggaacagtt
     1441 cagattctcg gcccaccatg cccgtcgcgg cgcaggccac ggcgatagat tgcggggaga
     1501 tcatcttgcc agttacgccg ccgctggtat ttgctgccac cagcagagtg tcagagacgt
     1561 tgatttgctg cgccgtggtc gattgcagtg aaccaaacag ggcgtttgaa gaagtgtccg
     1621 acccggtaag gaatacgccc agccagccga gaaacggtga gaaaaacggg aacatcacgc
     1681 ctgtacctgc cagtaccagc gccagcgtgg tggacatgcc ggaatagttg gtgacgaagg
     1741 cgaatgccag caccatgcca atcgacaata tcggccactt cagactaatt agcatttcgg
     1801 caaagacgcc aatccctttc ttgatgccca cgccgaggat gaagatagag ataatcgccg
     1861 caataaaaat agcggtgccg ccagccgaga gagggtcgaa tttaaacacc gcatccattg
     1921 gcgttggttg ggcgacaatg ggtgccgctt tcaacacttg ttgatgcaaa tgagggatct
     1981 ggaaattaat caccagtgaa taaaacgcgc cgcccggagc aaataacgct ttaaacggct
     2041 tcatggtcca gatggtgacc agcaccgtta agattaaaaa cggtgaccac gcacgaatga
     2101 tttgccccag actatattct gaaggcacgg gaccgccaga agatggctta tttaccacca
     2161 tcgcacctgc ggattgcccc atgctgattg ccgtttcggt atttttcggc cgccagactt
     2221 taagaaataa agcgagtgag acgatactca ccagcgccga agtaatatcc ggcagttccg
     2281 gaccaatata gttagaggta aagaactgag tgacagcgaa gcttccccca gcaaccagcg
     2341 ccgctggcca cgtctctttc acccctttcc agccgtccat cattgctacc agccagaacg
     2401 gcacaagaac cgacaggaac ggtaactgac gtcccgccat tgcgccaatg tggaacggat
     2461 cgattcccgt tacctgaccg gcgaccagaa tcggcacgcc caacgcacca aacgccaccg
     2521 tcgcaccaaa gccagcagct ccttccagca acgcaccaaa ggagaaacca atcagtaaca
     2581 cctgcaaacg ctgatcgtcg gtgatggaga taaccaagct gcggataata tcgaactgcc
     2641 cgctggcaac ggttaattta tacaggaaca ccgccgcgac aataatccac gcaattggcc
     2701 ataatccata aataaagcca tagcccgcag cagcaaatgc catatcaatc ggcattttaa
     2761 aggcgaatat cgcaatcagg atagataata taagggttat tgctccagcg acatgtcctt
     2821 tcagacgtaa taccgcgagt gcaacgaaga agaatattat cgggatcagg gcgaccagag
     2881 cggatagccc cagtcctccc atcggcatat acatttgggt ccaggtaacc atattgttca
     2941 gtctcttatt atctttatat gcttgatata cttaaggttg taataagcaa aagaggactg
     3001 aactgtaaaa tataggcgtt atactttaca gcaacagtac gccgctaacg caattgctac
     3061 ctctggcata acaagtatat cgggtaaggg tttctgttcc gcacacgcag gcgcagagta
     3121 tcgttaagat gtccatattg ttgttttagg cccgctagta atgcgctacg ggtatttaat
     3181 attgttaaac cctgataatc gctccggtta tttccgggat aaatgtacta ccgcagttac
     3241 tatcatagcc ccgacaataa aacttgccgg ggcttttttg acgctattaa tgactttctt
     3301 tttcgcgtaa acgccaggcg tgtaataacg gttcggtata accgtttggc tgtttcacgc
     3361 cgaggaagat taaatcgctg gcagctttaa aagcacacga gttagcgaaa ttccccgcca
     3421 tcggacgata agccggatcg ccagcatttt gctgatcaac cactttcgcc atattctcca
     3481 gcgacgcctg cacctgttct ttggtcagaa taccgtgacg taaccagttg gcgatatgct
     3541 ggctggagat acgcagcgtt gcgcggtctt ccatcagcgc cacgttgtga atatccggca
     3601 cttttgaaca accaatcccc tgctccaccc aacgcaccac gtaccccaga atcccctgca
     3661 cgttgttatc cagctcttgt tggatctctt gcgccgacca gttagcgttt tcagccaccg
     3721 gaatagtcag cagatcgtcc agcagcggtt caaattcagc actgaactcg gtctgggcaa
     3781 tgttggcttg cacgctctgt acgttggtat ggtggtagtg cagcgcatgg agcgtagcag
     3841 cggttggtga cggaacccag gctgtgtttg ccccggcacg cagttggtcg cccttctggc
     3901 tgtacatgtc tgccatcagg tccggcattg cccacatgcc tttaccgatt tgcgctttac
     3961 cgcgcagccc acagaacaga ccggaaagca cgttattacg ttcgtaggct ttgatccacg
     4021 gcgtcgattt catctggttt ttacgcagca tcgggccagc ttccatcacc gaatgcattt
     4081 catcgccggt acggtcgagg aaaccggtat tgatgaacgc cacgcggttg cgcgcctgag
     4141 cgatacagct acgcaagttc agcgaggtcc ggcgttcttc atccataatg cccattttca
     4201 gggtattcgg tgccataccg agcattgtct caatgcgggt aaacagtttg ttggcgaacg
     4261 ccacttcctg cggaccgtgc attttcggtt tcacaatata gacgctgcca gtgcgcgagt
     4321 ttttctgcac ttttaaatca tagagggcaa tcgcgccagt catgacgcca tcaagaatac
     4381 cttccgggat ttcattgcct tcgctgtccc aaatcacagg aatggtcatc aaatgaccca
     4441 cgttgcggat aaacagcaac gagcgtccgt gcagagaaat ttcagagcca tcggcggcgg
     4501 tgtactgacg atcgtcattc agtttacgta cgatttgccg accgtttttc tccattttct
     4561 cttgcagagt cccctgcatc aggcccagca gattgcggta cagcaggatt ttatcttccg
     4621 catcaaccgc cgcgaccgaa tcttcgcagt cgagaatggt actgatagca gcttcgacga
     4681 taacatcgtt gatgtgcgcc ggatcgtctt tgccaatccg cccgttggca tcaatttgca
     4741 gttcaatatg caggccgtta tttttcagca aaatgcaggt cggcgcagcg gcatcgccac
     4801 ggtaaccgac aaactgtgct ggagtacgta acgtggtttc tttaccattt ttcaactgga
     4861 tgcgtaattg tttatcgacc accttaaacg ccaccacatc ctgatagctg ccgttttcca
     4921 gcggtagtga ttcatcgagg aaacgtcgaa cccaggcgat aacctgctca ccgcgctgcg
     4981 gatcgtagcc actgaccatc gccccttcct gcgggatgat gtcgctgccg tataacgcat
     5041 cgtacagtga gccccagcga gcgttcgccg cgttcagcgc gtagcgggcg ttcattgccg
     5101 gaaccaccag ctgtggcccc gcctgactgg tgatttcgct gtcaatgccc gtggtttcca
     5161 ccgtcacgcg ctccggttgc ggcaccaggt agcccagttc acgaaggaaa gatttatagg
     5221 ccgctttatc ttttaccggc cccggattgc tgcgatgcca ctcatcaagc gccgcctgaa
     5281 tgcgatcgcg ttctgccagc aactgacgat tttctggtgc cagatcgtga acgatctcat
     5341 caacattgtg ccagaatgca gcagcgtcca gttccacgcc cggcaaaact tctccatcca
     5401 caaaacgttt aaaattggcg tcaatgcgta aacggccctg ggttatggtt tgactcattg
     5461 tttctctcct cgttttcgct tatttcgcca acaccgctgc tgccactttc gcgacctgcg
     5521 catcctgtgc tccggttaaa ccagaaacgc ccacggcacc aataatttgc ccatcaacaa
     5581 caaccggtac gccgccttcc agcgacgtca gtaacggcgc agtcacgaac gcggtacgtc
     5641 cgttgttcac catctcttca tagcccttag tttcacgacg ccccagcgcg gcggtacgcg
     5701 ctttctcctg ggagatgtaa gccgcaatcg gcgcgcaatc gtccatgcga cttaacgcca
     5761 gcagatgacc gccgtcatcg gcaacagcaa tggaaacaga ccagttattt ttctgcgcct
     5821 cttcctgacc tgcggcaata attgcactcg ccatttgctg gctaagaatg actttagttt
     5881 tcattttttt attccttttc aagggcttgt tctacaattt caatccagtg acgcacagag
     5941 gtacgaccgg cgctcgccag atgcgtctgg caaccaatgt tggcggtgac gatcatttcc
     6001 ggtttgccgc tttccagcgc attcattttg ttatcccgca gctggcgtgc cagatcggga
     6061 tgcgttaacg catatgttcc cgctgaaccg cagcacagat ggctgtcggg aacgtccgtt
     6121 aaggtaaatc caagacgaag taacactttt tccacttcgc cgttcagctt ttgcgcatgt
     6181 tgtagggtac acgggcagtg gaaggccagt tttttatcgc cgcgaattgc cagtttttcc
     6241 agcggttcct cgcgcagaag ttcgactaaa tcgaccgcca gttcactgac ctgacgcgct
     6301 ttatcggcat ataacgcatc gtttttcagc atctgcccat actctttgac gaacgcgccg
     6361 caaccgctgg cggtttgcaa aattgcctcg gcacctgctt caatcgcggg ccaccaggca
     6421 tcaatattat tgcgcgcccg tgccaaccct ttctcctgcg cattaagatg atagtccacc
     6481 gcgccacaac agcctgcttc gttagctgac atgacgctga tccccagacg atccagcact
     6541 cgcgcagttg ccgcgttggt gttgggcgaa agcgtaggct gggcgcagcc ttccagcatt
     6601 aaaacccgac gcttatggcg cagcggcgga cgcggtttag ctttcaccgt ttcagcaggc
     6661 agttttgctc tgacctgttc cggtaaaaac ggtcgcagca ccagccctac ctgcgtcagc
     6721 gcacggaaga ccgccggacg cggcactacc tggcgcaatc cttcgcgcag cattcgctcc
     6781 ggcagtgggc gtttcacttt ctgctcgaca atatcacgcc cgatatccag caaattgtga
     6841 tagcgcacac cagaaggaca ggtggtttca caattgcggc aagtgaggca gcgatcgaga
     6901 tgctcctgtg ttttaagcgt gacttcgttg ccttccagca cctgtttaat cagatagatg
     6961 cgcccgcgcg gcccgtccag ttcatcgccc agaagctgat aggttgggca ggttgcggta
     7021 caaaatccgc agtgaacaca ggcgcacagg atgctgtcgg cttccagcgc gcgttctgcc
     7081 gcatctcttc ggttaatcgg gtttgcatag cctgctcctc aaagttccgc gtacatgcga
     7141 ccggggttaa acacgccgca agggtcgagc tgctgtttaa gctgctggtg atagcggaat
     7201 aaaggagccg atagcggggc aaagccacca tctccggcac taaagcgggt cgcatgaccg
     7261 ccagcgttgc gggcgatgcg atggatttga ttgtcatcgg ctgtcgattt cagccagcgt
     7321 aacgccccgc cccagtcgat cagttgctcg ccgggtaaat ccatcatcgg cgcatcgctg
     7381 ggtaatgaaa tgcgccataa ggtacctggt aacgagaaga acggcagttg ttgttcacgc
     7441 aattgctgcc agaactgacc cgcaacctct tcgccaccca gcagttcacg cgctgctttt
     7501 accgatcctt cgccgccctc aaggcggatc cacaacgcat tgtcgaagta acataagcca
     7561 ctaatgggta atggctggag ttgccactcg gcgatttcac tcatggcttc ttgcaggctg
     7621 atttcccgac gcaggctcag agaggcacgc ggtcgcggta acactttcat tgagatttca
     7681 gtgagcacgc caagacaacc atagcttccg gccattaacc gtgagagatc gtatccggca
     7741 acgtttttca tcacttcgcc accaaaacgc agatgttttc cagtgccggt aatgatgcgc
     7801 gtgccgagga caaaatcgcg gaccgaaccg ctccacgggc gacgcggccc cgccagcccg
     7861 caggcgacca tcccgcccca ggtggcttct tcaccataat gcggcggctc gcagggaagc
     7921 atttgcccgg cgctctccag cgccgcttca atcgccacca gcggcgttcc ggcacgagcg
     7981 gttatcacca gctcggtcgg gtcgtaatta acaatgccgc gatgacaacg cacatccagc
     8041 gtttgcccgg tgactgggcg acctaaaaag gctttgctat tgctgccctg aatcaccagc
     8101 ggcgttttat cgctaattgc ctgattcacc tgctccagca gcgcctgact gtaatcacac
     8161 tcgcgtagca tcaaaagcgc tccagttcag gaaaaggtaa atgaccgtga tgcacatgca
     8221 tggcaccaaa ttcagcacag cggtgtagcg tgggaatgtt tttcccaggg ttcagcaaac
     8281 catcggggtc aaacgccgcc ttgaccgcat ggaaggtcgt gatttcatcg ctgttgaact
     8341 gggcgcacat ttgattgatt ttttctcgcc cgatgccatg ttccccactg atgctgccgc
     8401 caacttcaac gcagagttcg aggatcttcc cgcccagctc ttccgcgcgg gcaaattcac
     8461 caggttcgtt ggcatcgaaa aggattaacg ggtgcatgtt gccatctccg gcatgaaaga
     8521 cgttggcaac acgtaaatca tattgctgcg ataaacgggc aatgccttcc agtacgccag
     8581 gcagggcgcg acgcgggatg gtgccatcca tgcagtagta atccggggag atacgtccta
     8641 ccgccgggaa cgcatttttg cgaccggccc agaaacgtac gcgctctgct tcgtcctgtg
     8701 ccaaacggac gtcagtcgcg cccgctttca acaagatgta gttaacccgc tcgcagtctt
     8761 cctgtacgtc agactccacg ccgtccagtt cgcataacaa aatcgcttcg gcgtcgacgg
     8821 gataaccggc atgaataaaa tcttccgccg cgcggatcga caggttatcc atcatctcca
     8881 gcccgccggg gataatgcca ttggcgatga tgtcaccaac cgcaagtccg gctttttcta
     8941 ccgagtcaaa gctggctaac agaacccgcg ccacgggcgg cttcggcagc agttttaccg
     9001 tcacttcggt ggtcacgccg agcatacctt ccgatccggt gaacagcgcc agcaggtcaa
     9061 aaccaggtga atccagcgcg tccgatccca gcgtcagcgc ctcgccgtcc agcgtttgca
     9121 cttcaatttt cagcaggtta tgtacggtca gaccatattt caggcagtgg acgccaccgg
     9181 cattttccgc cacattgccg ccaattgaac aggcgatttg tgaggaagga tccggcgcgt
     9241 aatagagatt atgcggcgca acggcctagg agatcgccag gttacatacg cccggctgca
     9301 cgcgcgcgcg gcgaccaacg gggttaatgt cgaggctctc tttaaagcgc gccatcacca
     9361 acaacacacc tttttccagc ggcagcgcgc caccagaaag cccggtgcct gcaccacggg
     9421 tcaccaccgg tacacgcagg cgatggcaga cagccagaat cgctgtcacc tgttccattt
     9481 gcttaggcag aacaaccagt aatggacgcg tgcgatacgc gctcaacccg tcacactcgt
     9541 aaggaatgat ctcctcatcg gtatgcagga tctcaagtcc agggacatgc tcacgcagtg
     9601 ccatcagtac cgatgtgcgg tcgacatcgg gtaaagcgcc atcaagacgc tcttcgtaca
     9661 agatgctcat gagtaggctt cgctttgttg tgttgtgtgg cagctgattt ttgcgcgctg
     9721 cttctgtgaa cagttattaa gcgggctttt cgttttcgtc tatctcttta gctaccggtc
     9781 agaccatttt ttttccagct ctgtgacctt gtcttggtta acttaatgtt aaattgatgt
     9841 aacataatca cttgcgtgat gtgcgtgttt tgcgagttaa gaacagaaaa attggtccta
     9901 cctgtgcacg aggtccggga atgaaagatg aacgtcgccc tatttgcgaa gtggttgcag
     9961 agagtatcga acggttaatt atcgacggcg tactgaaggt cggtcagccg cttccctcgg
    10021 aacgtcgact gtgtgaaaag ctcggcttct cacgctccgc actgcgtgaa gggctgaccg
    10081 tgctgcgcgg gcgcgggatt attgaaacgg cgcagggtcg cgattctcat gtcgcacggc
    10141 ttaatcggga gcaggacacc agcccgctga tccatctgtt cagtacgcag ccgcgaacgc
    10201 tgtacgatct gctcgacgtt cgcgcattac tggagggcga atcggcaagg ctggcggcaa
    10261 cgctgggaac gcaggctgat tttgttgtga taacccgctg ttatgaaaaa atgctcgccg
    10321 ccagtgagaa ccacaaagag atttcgctga tcgaacatgc gcagttggat cacgctttcc
    10381 atctcgccat ttgtcaggct tctcacaatc aggtgctggt gtttacgctg caatcattga
    10441 ccgatctgat gtttaattca gtgtttgcca gcgtaaataa tctctaccat cgaccacagc
    10501 aaaaaaagca gatcgatcgc cagcatgcgc ggatctacaa cgcggtgttg cagcggctgc
    10561 cgcacgtcgc tcagcgcgca gcgcgcagcg cgcgatcatg tacggaccgt gaaaaagaat
    10621 ctccacgata tcgagctgga aggccaccat ttgattcgct cggcggtgcc gctggagatg
    10681 aacaaggtgg gaatgtgagc agagttttac cctatcccca aggcgagggg accgcccctg
    10741 cacttaacag gatctggtgt taaatctgtt catctatacc ctctcccgaa tgaccgcccc
    10801 tgcgctaaac aggatctggt attaaatttg cttttcatac aatcggtaac gcttgtacgg
    10861 ctccgcccca atgcgttcca gcatgttatt catgcctgta ttggtttcga ggatccatga
    10921 catctccagc gcatcgatct tccggcgggc aaacggatcg cgtaaggctt caatcaataa
    10981 cagcgcaatc accgggccga tgcggctgaa ctgatactcg tcgcgcacgc ccatcagcgg
    11041 tactcgcgca gttcgcacac cgctgacttt caaacgccac agcaattttg cccagccgaa
    11101 gggaaagagc gatccgttca gatcggcaat cgcctcgttg atgttaggca agccacaata
    11161 aacgcgcagg gtgcagaatc aatctcagcg atgtagatca tatcgtccgg caccagatat
    11221 ttaagttggt cgcccatggt cgcgaattca tgttcggtaa acggcacaaa tccccagttg
    11281 tgctgccagc cagagttgaa aatctcacgc aggatctgca tctcttcggc aaaccgctga
    11341 cgattgatgc agcgaatggt cacctttttg cgcacctgat ccatcagttt ttttagcgcc
    11401 ggagagaaag tgagatcggt tcgctgcatc caccacgcca gtaaatcaat gcctttgtga
    11461 taacccagtt gttcaatatg cgcggcatac cacggtttgc cgtgtggcat catcgcacag
    11521 ggtggtgtgt caaaaccttc aatcagtaat ccgctttcct gattgatatt caggctgaaa
    11581 ggcccgctga tcttacttgc accttgtgac ttcaaccacg cttccgctgc gccaaacaac
    11641 gcggcaaaat cctgcggatc atcaatggcg tcaatcatgc cgaaatgacc ggtatcttca
    11701 ccgtaacgct cacggtgcaa ggtatctatt tgcgcggtaa tacgcccaac tatctgcccc
    11761 gctttttttg ccacccacgc ctgccagatg atatggtccg tccccggatt tttcgcagac
    11821 aaatgctcgt tgcgttcaat gaataaaggg ggtatccagt ttggatcgtc gggataaagt
    11881 gatgacggaa aagcgataaa tgccttaagg tcatttttat taattacttt ttcaatttta
    11941 atcatcagca gatcgcattc catatctctg tggataacaa ataattaatg gtgaaaaaca
    12001 ggttgcaaca gcaacgagcg gcatcttaat aatcaactca atgaatacaa acgacgaata
    12061 aatagtatta attaatgatt atgataaggt cagaggctac acagaaacgc ctcaatgcct
    12121 tttgtcaatt taaggcgacc ggacaaggct tcttcttaat atattcataa attaaatatc
    12181 attcataata aaaacaaaaa acttaaactc attatttaaa tcaatgttca ttttcacacc
    12241 gatgaaaata ctatctcatt tatcttttat gcctatataa tccaccgcat tattgtcatt
    12301 ttatttttga tatccaattt tttcgtggtg tatatgtcta ataaaatctt tacgcattcc
    12361 ctacctatgc gctatgccga ttttccaacg ctggttgatg ctttggacta cgccgctctg
    12421 agtagagccg gaatgaattt ttatgacaga cgttgccaac ttgaagatca actggaatat
    12481 cagacattaa aaacgcgtgc cgaagctggt gcgaagcggt tgttatcgct gaacctgaaa
    12541 aaaggcgaac gcgtggcgct gattgccgaa acgagtagcg ggtttgtaga ggcttttttt
    12601 gcctgtcagt atgctggctt agtcgccgtc ccgttggcga ttccaatggg cgtcggtcag
    12661 cgggattcct ggagcgccaa actccagggt ttactggcaa gttgccagcc cgcagccatt
    12721 atcactggtg atgagtggtt gccactggtc aatgccgcga cgcataacaa ccccgaatta
    12781 catgttttaa gccacgcttg gtttaaggca ttaccggaag ccgatgttgc gctccagcgt
    12841 ccagttccga acgatatcgc ctacctccag tacacctccg gcagcacccg ttttccccgt
    12901 ggcgtcatta tcacccatcg cgaagtgatg gctaatctac gtgctataag ccacgatggc
    12961 attaaattac gccctagcga ccgctgcgtc tcctggcagc ctttctacca tgatatggga
    13021 ctggtcggct ttctcctgcc ccccgtcgcc acgcaacttt cagtagatta tttgcgcact
    13081 caggattttg ccatgcgtcc tttgcaatgg cttaaattga tcagtaaaaa tcgcggcacc
    13141 gtttccgttg cgccgccgtt tggctatgaa ttgtgccaac gccgcgtgaa tgaaaaagat
    13201 ctcgctgaac tggatctttc ctgctggcgc gtcgctggta ttggtgctga gccgatctcc
    13261 gcagaacaac tccatcaatt cgctgaatgt ttccgtcagg ttaactttga caataaaact
    13321 ttcatgccgt gctacggact ggcagaaaat gcgctggctg tcagcttctc tgatgaagcc
    13381 tccggggttg tggttaacga agtggatcgc gacatcctcg aatatcaggg taaagccgtc
    13441 gcgccgggtg cagagacacg cgccgtatcg actttcgtca actgcggcaa agcgttgccg
    13501 gaacatggta ttgaaatccg caatgaagca ggtatgccgg tcgcggaacg tgtggtaggc
    13561 catatttgca tctccggtcc cagtctgatg agcggttact ttggcgacca ggtttcgcaa
    13621 gacgagattg ccgcgacggg ctggttagac accggcgacc tcggttatct gctggacggt
    13681 tatctgtatg tcaccggacg cattaaagat ctgattatta ttcgtggccg taatatctgg
    13741 ccgcaggata ttgaatatat agcggaacag gaaccggaaa ttcattctgg cgatgcgatt
    13801 gcttttgtta ccgcccagga aaaaatcatt ttgcagatcc agtgtcggat cagcgacgaa
    13861 gaacgtcgcg ggcagcttat ccacgcgctg gcggcacgga tccaaagcga atttggcgtg
    13921 accgcggcta tcgatctgtt gccgccccac agtattcccc gaacgtcctc cggcaagcct
    13981 gcccgtgcgg aagcgaaaaa acgttatcag aaggcttatg ctgccagtct taatgtgcag
    14041 gaatccctgg cataaatcaa actgtcgcgg tgacgggcgc taccgggttt atcggtaaat
    14101 atattatcga taacctgctc gcccgcggct ttcacgttcg cgcattgacg cgtactgccc
    14161 gcgctcacgt caatgataat ctcacctggg tgcgcggttc gctggaagat actcattcac
    14221 ttagcgagct ggttgccgga gccagcgtag tggtccattg cgccgggcaa gtgcgcgggc
    14281 acaaagaaga gattttcacc cgctgtaacg ttgacggcag cctgcgcctg atgcaagcag
    14341 caaaagagag cggcttttgc caacgttttc tgtttatctc ttcgctggcg gcgcgccatc
    14401 ccgagctctc ctggtatgca aaatccaaat acgtcgccga gcaacgactg gcagcaatgg
    14461 cggacgaaat tacgctgggc gtttttcgcc cgacagccgt gtatggtccc ggcgataaag
    14521 agttaaaacc gctgtttgac tggatgctac gcggcctgct gccacgactt ggtgcgccag
    14581 atacacaact ctcttttctt cacgtcaccg attttgcgca agcagtgggt cagtggttaa
    14641 gcgccgaaac tgtacagacg caaacctatg aattatgcga tggcgtcgcc ggtggctatg
    14701 actggcaacg cgtacgacag cttgccgcca aagcccgttg tggttccgtg cgaatggttg
    14761 acattcctct gccggtactc acaggccttg cggatatcag taccgcgttg agtcgcctgg
    14821 caggtaaaga acctatgctg acccgctcga aaattcgtga attaacccac gccgactggt
    14881 cggcaagtaa taaccgtatt tctgaagata tcaattggtt tcccgggatt agcctggaac
    14941 acgcattacg caacgggcta ttttgattgt cttgttttaa aggtgtgtta acgtttctat
    15001 ggtaaatcgt gaaatagtaa tggattacat cctttcctgt ttacaggatt tggtcgaaaa
    15061 cggtgtggaa attaagcccg acagcgatct cgtcaacgat cttggcctgg agtcgatcaa
    15121 agtgatggat ctcttaatga tgctggaaga cagattcgat atttccattc ctattaatat
    15181 tctgctggat gtcaaaaccc ctgcgcaatt aatggaaact ctacttccat ggctggagaa
    15241 taaataatgg ggctatacga taaatatgcg cgccttgctg gcgagcgtct gcaatttagt
    15301 gacaacgggt taacaccgtt tggcacctgt attgatgaag tgtattccgc caccgaaggc
    15361 cggatcggca ataaaaaagt gattctggcc gggaccaata attatctcgg tttaaccttt
    15421 aatcatgatg ccatcagcga aggtcaggcc gcgttagcgg cccaaggcac cggcaccacc
    15481 ggttctcgta tggcaaacgg cagttatgcg ccgcacctgg cgttagagaa agagattgcc
    15541 gagtttttca atctccctac cgctatcgtt tttcaaccgg ttataccgct aatttaggcg
    15601 tcatcagcgc acttgccgac cataacgccg tggtgttact ggatgccgac agccacgcca
    15661 gtatttatga cgcctgttct ctcggtggcg cggaaattat tcgtttccgc cataacgatg
    15721 ctaaagatct ggaacggcgc atggttcgcc tcggtgaacg ggcgaaagag gcgattatca
    15781 tcgttgaggg catctacagc atgctcggtg acgttgcgcc gctggcggaa attgttgata
    15841 tcaaacgtcg tctcggtggt tatttgattg tcgatgaagc gcattcgttt ggcgtgttag
    15901 gcgcaacggg gcgcggactg gctgaagctg tcggcgttga agatgatgtg gatattatcg
    15961 ttggtacttt cagtaaaagt ctggcctcga ttggcggttt tgctgtcggt tcggaagcga
    16021 tggaagtgct gcgctacggc agccgcccgt atatttttac cgcctctcct tctccgtcct
    16081 gcattgcgac cgtgcgctcg tcgttaagaa ctattgccac acaaccagag ctgcggcaaa
    16141 agctgatgga taacgccaac catctgtatg acggtctgca aaaactgggt tatgagttaa
    16201 gttcacatat tagcccggta gtgccagtaa taatcggttc gaaagaggaa ggtttacgca
    16261 tctggcgcga gcttatctcg ccgggcgtgt atgtgaatct tatcctgccg cctgccgcgc
    16321 ctgcaggcat cacgctgctg cgctgtagcg taaatgctgc ccacagtcgt gagcaaatcg
    16381 atgccatcat tcaggcattt gctactctga agcagtaatg tgagcccgcc gtggtgtttg
    16441 cgccacggcg ggtttaatgt tgtctgtcat accaatacac caggctcaac gccaccaaca
    16501 caaacgctac cggtaaaccg agcgcggtaa caggtgcgct taacgcaaat agcaatccca
    16561 gattaaccat gatttgatag ctgatccagg tgagtaagcc cacgatgacg cctacagcga
    16621 gacggctacc catcccaggc gaacgcgggg cactaaaggt aaacggcaca gccagcaaaa
    16681 tcatcgccag ggtcaggatg ggttgcccca gtttttccca cagtgcaagg cggtattcgc
    16741 tgctgggttg cccggtttcc tgcaaataat ggatgtaatg attaagctga cggacagaaa
    16801 aagtattccc cggcattgat aactcttcaa gattcatact ggtgaaggcc gattgccagg
    16861 caagattatc taatgtttcc agcgtttctt tgccattcag ccatttttta tgattcacac
    16921 catgcaggac ccacgttttg tcgtcttcaa tggtggcact gcgtgcataa atgtaggatt
    16981 caagcgagag atcgtcgcga taatgaaata tctccacgcc caccggctgg ccctgctcgt
    17041 tcagcgattt caccgtcaca aattcattac cacgcctggc ccacagcata ttgccggtaa
    17101 tgtcatcgtc ctcccccaac gcggtggcgg tcgatttgat ttgcaacgcc tgttgctgca
    17161 atggcgacgc cacccactca tctatcgcgc ctaacgaaac agtccacaat atcccggcaa
    17221 ccagcgccac cagtgcaata cggaagatag aaaaccccgt gctgcgaatg gcggtaagct
    17281 cactgttttt cgataactgc cccagtccaa caataccacc caataacgcg ataaacggcg
    17341 aaagttcgac cagtgtgcgc ggtaaggtca ttagcaccac cagcaccgcc tgagtccagc
    17401 gataaccgcc cgggctgaca ccatccagtt cgttaatcag gttgaaggtg gtaaaaagcg
    17461 gcagcaatag ccctgcagcg gcggcaaaac cgagaaagag atgacggatt aaatagcgac
    17521 tgaaaacatt catcgggaaa atttctgcag caaagagaag tcacgtgcga ccagtatcag
    17581 caggcccatt agcatgagtc cgggcactaa ccacaaacca ggaatgaggg gaatcgcgcc
    17641 gttagccacc agtgtacggc agacgtcgcc gccataaaaa atggcaataa acagcaacgt
    17701 cagtggcaat aacgttgaaa atcgcccttg ccgcggtttt acccgactta atgaaatggc
    17761 cagcagcgcc atcaacaatg cggtcaggcc acggctttgt cgccattgca gttcggcatg
    17821 atcggcagga aaggcggagc gcgccagctc cgtgaccgat ttcgctttgc gtttgacgtt
    17881 cgggctttga tccagcggat tcagatgtaa ttgcagatta cggtagatct gttcgttgtc
    17941 gtcacgcccc tgatgatcga gaagataggc agtgccgttg tgcaacataa cggtcggttt
    18001 ctcgggtgat gggtcaacca catcaaccga accggcgcgg aaaatacggg ttcgattggc
    18061 agtagaagta taaatcagcg cgtcagtcag gcgattatta tcctgatcaa ccgtctgcga
    18121 aaggatcatt cgtccgttat cgttagtgtt aaatttcttt gcccgcaact ggcgaacatc
    18181 cagctccgac tgcgactgct gctccagttg ataaatctgc gcataagccc acggacgacc
    18241 atacatcgac agaagggtga caaaaatact tagcggtatc gccagataaa gaacggcttt
    18301 gtacaagcgt cccggactgc cccccgccgc agagatagcg gtaatttccg agtcggtata
    18361 catctgccct agcgtcacgc caactgacac atacagacca acaggtaaca acatctccag
    18421 tgcaatcagt actttgtaga aaacgatatc cagcacaaca tcaagagcta atgtcccatt
    18481 tgccgcttcg gtcagataac gctgtgcgga gtagctggcg aaaataaaga tcagaaaacc
    18541 gacgataatc agcaccagac ggcgcgtacc acgcatgatg tagtgttcaa ccagcttcat
    18601 ctcactaacg ccctcaatcc tttgagtttt ttattcattg atcagtttct gcgccaacgc
    18661 aatgacatga tctacctctt ccggcgtcag gctgccgtcc tttgcccact gaatttgccc
    18721 ggctttattc agcaccagaa tggttgaact ctgctccggc aagcgccagg caacgcgtcc
    18781 ctgtccattg ccgtcaataa cgaattgcgc ccacggatag cggcgtttat ttttttcgat
    18841 tttgccaaca acaaaatagc cagtaccaaa gatggcatca tcggtgttca caatggtggt
    18901 gggctgaaaa cgatcctgcg gaaagtttgc cgcttcaacc gctttaatta acagtgagtt
    18961 ctttttcttc gccgacttac gcccggcgat atactgaata atgcggactt ttcccgctaa
    19021 ttgtgtgctg ttccactttt ggtagtgaat ttcgtcgtcg ctattcagaa gcagctcgcc
    19081 gccttcctgg atataaacag gggtaaccgt tttgccagtg acgaaattgt gtgcctgcac
    19141 acccggggct aagagcatga taatcaacgc aataatcaaa cgagatgaca tcattgctcc
    19201 ggctatagcg gaaaacgcga aatcagcagc tctaagcgct gacaaatatc gctatccagc
    19261 gccaacggtg cataatacga ctggcggttt tccatatcat ccgccaccat gtcgtcgtgt
    19321 ttatctccca tcaggccatt aatggcggcg aggaaacggg aaatctgtaa cgcctcttta
    19381 ttttcaggat caagcgatct taaggtgtcg taataagaaa cctgctggct gatgattttt
    19441 cgcacatcat gaaattccct gccggttagt cgcggtttgg caataatctc tttcagggta
    19501 tgcatttgct tttgccgata ggcaataaaa ctgcttacgc tttctaactg gctatggcgt
    19561 aatgaatagt tcaccaacga ccagaccgga gagcttaaat aaatccgtaa caggttgccg
    19621 taatacgaca caatattttt cttaccgtta cgaaagccgt cctgaaaacg ccccagaaaa
    19681 acggtcgttt tgccgaataa aaatccggcc tgatgcttct ttaaatacag ccgttgcgca
    19741 aaacgcaggt gcttgtagcg cgctcgcatg tatttaaatt gtttgcgctc atcagcagtc
    19801 agctcatcgc ctttggcttg tttgcgaact aaacgcagta attcctgacg atcaacgcca
    19861 ttgagccaga attgcaggca taacgcgtaa ttatcatgaa tgatttcggt ggtacaactt
    19921 agtgcaatag tcggcggcaa actaacctgt gcatcaacga catcatcttc ttcaatcgca
    19981 acaaatagac gctcgctcgc ctcaagcggg aaatattttc tcatcatcag gatttctctc
    20041 cggctgcgtt ataaagcaag gaatggcgct gaaaaaatgc ctttaaggtt ggaattaacg
    20101 agagcgccac gtccagacat tgcccaccga agtaagcaaa cgctgctccc agcacaccaa
    20161 acagatgaca gaacatgaac agcaatacga tgtagccgat tgaggcgatg gtttgcgcca
    20221 cgagaaaagc gcgctgtttt cccgccatca atagcaatga ctcctgcggg aagcccagca
    20281 tcgaaatcac aattgcgccc agcatcacct gaatcaggtc atacgcttcg agatacttaa
    20341 cgccaaacac cagcgaaatg agcggcttgc cgacgatcaa caccgcgaga gccaccagaa
    20401 taccgattcc gcccgccagt aaaccggact tcacacctaa caaccacggt ctggtggtgc
    20461 gcggatctaa acgcatcacc tctgggtaaa aacttttacc cagcaaaccc gctggcgttc
    20521 cggcggcgtc gaagaatgtc atggcgattt taaataaccc ggcggcagcg ggtcctaaca
    20581 cgatccccac caacactgtg ctgcacgagt tacgcgccga ccagatggag tgggcaatgt
    20641 tggttgacca gacaaaactc cacgcgcctt taatgtgtcg ggcagactca aacagattca
    20701 atttgaaggc gttatggata tttctacggc gtaattcgcg cgcggcaaac caccagtaca
    20761 tggtgccgcc aaccagattc gacacgtacc aggcaataac aaaacccgca aaaccaaagt
    20821 caaaatacca ggctgcgacg ctccccgctg cgcgcagaaa aggtttcgtc gcctgctgta
    20881 cagcaatcaa atcaaagcga tctaccgcac gcagaatgcc ggtcggcgtg gaggaagcca
    20941 ttgaaggaat gagcgtgcaa tacagcgccg ccagccaaaa gctttggtca tccagaccta
    21001 atgaatggga aaggaatggc agtaaggcaa tgccgccgac aatcgccacc gcgccgctga
    21061 cgatatccag cgagaaggaa aatgagacga cattgcgaaa ttgctgcgga ttattgttgg
    21121 tcaaggctgg cgttccgtac tgaaccacca gctgccatgt ctgaaactta ataaaatcgc
    21181 tgatcgactt ggcgtacgat tgcacaatca ccagtacacc aaacatggcg ggcgtcatcc
    21241 ctttaccggc acacgagagc gccaacagcc ccagcaaggc gctctcgaca ttactggagc
    21301 ctaaccaggc gctattgcga ataatggtgc gaaacgcgcc atctgcaaac caatgtttga
    21361 tgttaaaacc cgccaattca gcctgacctt atcgttgata gtaaaaaata tcccgccagc
    21421 cttaagttaa acttcggcgg tcagaaacga tggcaaccag agaaaccgcc ttctgtacct
    21481 gttccagcac ttcgctgtag ggcgctctgg aatcaatctc aagaattttt gtgccgttat
    21541 agccaatcgt cgacatgacg ccgattttgt cctgcagctc ggcatactca tggtcaggct
    21601 tgcgggagat ggcagtatca atatcaatcc ccaaacgaat gattaattca ggacggtatt
    21661 gcgccatttt ttggtataaa ctccgttcgc gctgcgccag aaacatgctg attttcccgg
    21721 tcgcacgttc gacgccgatc cccggtccgt cataataaaa gcccgaaatt tcagcctgcg
    21781 ggaagcgatc gctgaccacc agaacgccac tttgcgccag tcgctgaacc ttgcgtagat
    21841 tcgccattct tcgcagcgag aagcagtaca taatcaccgc cgcccatagc gccggagatt
    21901 tggttttcat gctttgggtt ttcgaggatt tggccgccaa tcgccgttcc agccagacgc
    21961 caaccaacgg caatcgtttg attttgtcgc cgtcttcgcc ggagagcagc cccagatagc
    22021 ggcgctcggt ttgccagtgt tgttgcagcg atttcaccag gtcggtggtc agcgtggatt
    22081 taccggtgcc atcacacccg accaccgcaa tcagccccgg aatgtagttg ggctgcggcg
    22141 cggttgtact attaacagtt tgtgtttgta gtgcatccat tcgttaaatc atcccctatc
    22201 cattttggtg atcaggaaag cgcggcgtga attgcgcgta gtgacgcttg cataatttca
    22261 tcggctggtt gccgaccatc cagttcaagg atcttggcgc cattgaatgt cagttgcggc
    22321 gtaacggcga ttttttcctg taacgctgcc agttggtggt caggtttacg cgcaaacgcg
    22381 gtttgttcat caatgccaag acgaatcaac aatacgggta aataagacgc catccattgg
    22441 tacagcttca actcgcgctg ccttaacatt tttatccaac cgttaccgcc cgtggttttt
    22501 gccaattgcg ggccatcaaa gcgaaaaccc ggcacttcaa cctgcgggta gcggtcggtg
    22561 atgagcagat agccttgctg gcttttacac aacattttgc gaaacttgta cgcccgccag
    22621 caggaaagca gatagataac cagcgcagta atattgccag gcggagttaa gggcttttcg
    22681 tgtacatgtg ccgctttact tcgcagataa cacccacaag gtgcgccaat aacagggagc
    22741 tgtgaaatcc attcgccaat tcgcccggac gattgcccga gataaatgtg ttctgttggc
    22801 attcttgctg ccagttcatt taccaggctt gccgtgaggg tcgatttacc tgaaccatca
    22861 catcctacaa tggcaataac acgcactggc gtggaattaa ttattgacat attacgttga
    22921 ttcacgaaaa acccggcagt aaataatgta ttgaatatta cgtggtccgt gctcagaata
    22981 tccgttcaac cttattgtga caatggctaa ttgtgacatt gacctgaatt acgctttcat
    23041 aaaaacatat taaccaaata aatattttta atggatattt aaattaaagg atatattcat
    23101 gcagtcaata acacctccat taattgccgt tattggtagc gatggttcag gcaagtcaac
    23161 ggtgtgtgaa catcttatta ccgttgtcga aaaatatggt gctgccgaaa gagttcattt
    23221 aggaaaacag gccggaaatg tcggtcgtgc agtgacaaaa ttacctttga tgggaaaatc
    23281 cttacataaa acaattgaac gaaatcaggt gaaaacagca aaaaaattgc ctggaccagt
    23341 tccggcgctg gtaattacag cgtttgtcgc ccgtcgctta ctgcgctttc gtcatatgct
    23401 tgcctgtcgt cgtcgcgggt taattgttct aaccgaccgt tatcctcagg accaaattcc
    23461 tggcgcttac gatggtacgg tgttcccacc taacgttgaa ggtggtcgtt ttgtctcatg
    23521 gctggcaagc caggaacgta aagcgtttca ctggatggcg agccataagc ctgatctggt
    23581 catcaaactc aatgttgacc ttgaagttgc ctgtgcacgt aaacccgacc ataaacggga
    23641 atcgctggcg aggaagattg ccataacgcc acagttaacc tttggtggtg cacaactggt
    23701 tgatatcgat gccaatcagc cactggaaca ggtgttggtt gatgcagaaa aagcgattac
    23761 ggattttatg accgcgcgtg gttatcacta gtcaaaaatg gaaatgcccg atcgccagga
    23821 ccgggcattt tcaggaaggt taaatcaact gcaatgctat ccagtacagc ccaccagaaa
    23881 gaaaaattgc cgccggtaaa gtaaacaccc acgccatcag gatgctggtt accgttttac
    23941 gctgtaaccc accgccgtcc accaccatcg tccctgctac cgcagacgag aggacgtgtg
    24001 ttgtggagac aggcatccca atataactgg caaggccgat agacactgcc gccgtcattt
    24061 gtgccgccat gccttgcgca tacgtcatgc cgcgcttacc aatcttctca ccgatggtca
    24121 tcgctacacg acgccagcca atcatggtgc caatgccgag cgccagtgct accgccatga
    24181 tgatccacac cggagcgtac tcaatggtgc ttaacatatc gctgcgcagt tttttcagca
    24241 ggttctggtc ttctttactg acgcctggca gtttcgccag cttcgcggag gtatcggaga
    24301 tgcacagcat aatgcggcgc agctggctgc gctgactcac gcttaacggc tcgtaacttt
    24361 ccatattgcc tggcagcatc gttttaacgc gcgcaatcgc atcaaaggta tttgccggat
    24421 gacagtgaaa ctctgttact tgcgtgccat cagtcgatgc tgcaggcaat ggaggttcca
    24481 tcgcaatcaa cttctgcggc agttcaggat gctgttgcag gtagtgttcg aagttggtaa
    24541 cggcatcgcg ggtacgggta atttcatagc cggacgcatt catattgacg acgaagccag
    24601 caggggcaat ccccaccagt accagcatta ccaggccgat ccctttctgc ccgtcgttcg
    24661 cgccgtgcga aaacgccacg cccgcagcgg aaacaatcag cgcaatacgc gtccagaatg
    24721 gcggtttacg tttgcccttt ttctttttgc gatcttccgg aatgcggtga atacggtcac
    24781 gctttttcgt cccactccag aaacggcgca gcaggaaaat cagaccaccc gcaatgacca
    24841 ggccgacgat aggggaaaca atcagcgagg agaaaatttt ggttacttca cgcaggttta
    24901 acgcatccat caccgatgag ccggttaaca gcgcgttggt taaaccgatg ccgataatcg
    24961 caccaatcaa ggtgtgcgaa ctggaggccg gtaaaccaaa gaaccacgtt cccaggttcc
    25021 agataatcgc cgccagcagc atggaaaaga ccatcgccaa accgtgagtt gaccccatat
    25081 tcagcaacaa atcgattggt aacatatgga caatggcata ggcaacgcta agtccgccca
    25141 ataacacgcc aaaaaagtta aaaaatgccg ccatcaccac agcaagttgt ggctgcatgg
    25201 cacgagtata aataacggtt gccaccgcat tcgccgtgtc atgaaaacca ttgattgctt
    25261 cgtagaacaa cacaaatgcc agagcaagta ataacaaaag ccccgtatat atatcaaggc
    25321 caacaaataa atttagcata tattagatta cgccattttg aatttacgaa cggacgcatt
    25381 atcagtgact ttaacggcat gggcaaagtg aaatatcatt ttttaacgat aaaaagagtg
    25441 ttatttcttg tgtatcagac aataagcatt cattcaaaac ctttagaaat aatcaaaaca
    25501 caggtattcc actggttaat atattcacat atgaaatgaa taaatattgg aatatataaa
    25561 tattgaatat tttgattaat ccccgtacta attattcttc ataatcagta cggggttgct
    25621 acaacatgta ttactttttc accacaatta acggttcaat atcactctct tttttgatga
    25681 ccagtgattc atcaccgcgc aaacacgtcc caccgtagtt gccgccaacg gtgaaggtac
    25741 atacctgaat gtatttaccg tccactttcg gcaaacacca cagttgctga tagatgtttt
    25801 tctgctcggc aaatttaccg ctggttttgt ccagcacctc ttcatgatgg ctgaccaggt
    25861 cgatattgct accacagcga ccggcgatcg gtttcactgc gtaacctgtt ttcaccaatt
    25921 catcattaac agtgaaatcg gtatccagca ggtaacgatg gtgcgggaac agcgaccaga
    25981 ggatcggcag aatcgctttg ttgccgggga tcaccgtcca cagcggctca aagaccagca
    26041 cttccgggcg cagcaatacg tcgataagac gcacttcgtt ttgcggatga ccggtacgga
    26101 ttggcaccgc agcaaactca cggtcgctaa cttcacgaat ctgatcaaac gcggtttccc
    26161 acgcccaggt tttccacacg cagttaacca gtcgcccttc cccatcaatc agttgcccgg
    26221 cagcatccca gcccagttca tccagcccgc gcaagatacg cgtttcaaag cccgcctggt
    26281 gcagcgcctg ctccataaac tgcgcgtgat agttttcctc gatatctttg tcctgcatga
    26341 tatggacaaa cggacgtgca cgactgtgtt tccaggcacc agccagttcg ttaatcagcc
    26401 cttccgccgg attgaagccg ttgcctttat agccctgctc cgcccaacgt tcgaggatca
    26461 agcccgcttc ggtatgacag gaggcggagt cggcgttgta ttcgtaaacc ttcaggccac
    26521 gctcatccat gcagaaatcc atacgaccag tgatcatatg gtgacggcga cgctgccagg
    26581 agagacgcaa acgtggccag aggattttcg gaatgtcgaa cagcgccagc aggttgtcat
    26641 ctttcagcac cttgtcggtt gcgtgaagat acatcagatg cagctcgttg gtggctttaa
    26701 ttagctcctg ctcggcactc tcggtaatgg tgtagtaatg atacggatcc tgattgatca
    26761 cctgaccgtt ggcctgcaca taggcgtttt gtagcggatc tttttcatcc agccatttac
    26821 cgtcaaactg gcctttgttt tccagacgcg ctccactgat tttcagcagc tcgcctgcaa
    26881 tttccggctg cggtaagctg tattcagtat cttccgtctg gatcatccag cccagaatgg
    26941 tggtgtcatc aaaagtgtct ttaagggtat agcagccgtt ttcgaccacc atctccagct
    27001 cgcgcgtcca ctgttgccct tgcggcaacg gggaatgaat cacgttctgt tccgcaatac
    27061 ggactttgtt gccatgcaat tgggtaatga tggcgacatg gccagtgtct ttaaattcac
    27121 cgcctttatc ccagataaga agcgcacccg cgaccggcgc acgcggcgag ccgttaggaa
    27181 atgcctgcaa tggcaggatg ttgtcattaa ccacttctcg caggaagcgc agcgagaaaa
    27241 tctcccacgc catacccacg tcagtaaaga ccacaccgta attcagaaag agaaaacggc
    27301 gagcaaattc aacgcattgc cacttgtggc ccatatattc gtcgtcgata tagctacgga
    27361 atacggcgtc atcttcgtat tcctgcggat cgagagaact gtaatctgaa gagtagattg
    27421 ctaccccacc tggggcgtag cccaataatg tcccgaacgg ggcatcctgg ctggtcgttc
    27481 ctttgctcat cactttacct ttaacaatac aacctaagca gttggcgtaa ttgttgtgct
    27541 ctgattacct gctcagcgac attaaccgga cagaggtcag actaatcata cacctcatcg
    27601 cagcggctgg cgcaaagggt taaaaaattc acattctgta cagcaagtga cctgctacac
    27661 tgcttcaaca ctaccactca gaaggcaact cactatgaca gacaatactt atcagcccgc
    27721 gaaagtctgg acgtgggata aatccgctgg cggcgcgttc gccaatatca atcgcccgtt
    27781 ttctggtccg acgcatgaaa aaacgctgcc cgttggcaaa catccattgc aactttattc
    27841 gctgggaacg ccgaacggtc agaaagtaac gattatgctt gaggagctac tggcgctggg
    27901 cgttactggt gcagagtacg acgcctggct gattcgtatt ggcgatggcg atcaattctc
    27961 cagcggcttt gtcgaagtga acccaaactc gaagatcccg gcgctgcgcg atcatacgca
    28021 taatccgccg atccgcgtgt ttgaatctgg ttcgatcctg ctttatctgg cggagaaatt
    28081 tggctacttc ctgccgcagg atctggcaaa acggactgaa acgttgaact ggctgttctg
    28141 gttacagggt gccgcgccgt tcctcggcgg tggttttggt cacttttacc attacgcgcc
    28201 ggtgaaaatt gagtacgcca tcaaccgctt taccatggaa gccaagcgcc tgctcgacgt
    28261 gctggataag caactggcgc agcataagtt tgttgcgggc gatgagtaca ccattgcgga
    28321 tatggcgatt tggccgtggt ttggcaacgt ggtgctcggc ggcgtgtatg atgccgcaga
    28381 gtttctggat gcgggaagtt ataaacatgt gcaacgctgg gcgaaagaag tgggcgaacg
    28441 tccggcagtg aaacgtgggc gtattgttaa ccgcaccaac gggccgctga acgagcagtt
    28501 gcatgagcgc catgacgcca gtgattttga gacaaataca gaagataagc gtcaggggta
    28561 agggttggtg ttcgtcgcag caagccatcc aggccggata aggcgttcac gccgcatccg
    28621 gcaatcgtgc acaatgcctg atgcgacgct gccgcgtctt atcaggccta caaaagctaa
    28681 tcatcacgcg ctggtaatgt cgtaatccat ttggcgcagt gcgtctaatg tggctttggc
    28741 ttcatcttcg tcgatgatgc tcatggcaaa tccgacgtgt accagcaccc actggcccag
    28801 cagatcggca gggttacctt cgcaaatcag ggcgatattc acgtcgcgct tgataccaca
    28861 tacttcaacc tgcgcaagct ggtgaatatc ttcaccgaca gccagcacct ggcctggaac
    28921 gccaatacac attgttaact ccggttattt cgattgccgg acaaacgcga aaaagaacct
    28981 ttagcgggtg ttccctctca ccctaaccct ctccccggag gggcgagggg accgtccgtg
    29041 cgctttttag gcgttgtcct cagtcttatt caacttcaat acttttgacg atcagcgaat
    29101 cgccggtatc gacacgcaac cgctcgccgt gacagagcgg acactgcgca tcgtgctgat
    29161 gaatctccac cacctggctg caatcccagc accaggcctg ggcgggttta tagacgatat
    29221 gtaaatcgca cccttgcgcc accgttccgt ggcagacaat ttcaaaacta aaacggacgg
    29281 cgctctcctc aacgcaggag agcgcgccaa tttccagcca cacggcggtg acgcgcttaa
    29341 catcgtgctg ctccgcctgc cgttggataa tttcaacggc gctctggcaa agcgacaact
    29401 catgcatttt cgctcctcct gcggccaaac agcagggcgc gacgccctgc gtgtggtaca
    29461 tccggattgg tgactggcaa cgaaaggatc attcgtgcgc agtcatccgt caggcgttgg
    29521 ccttcttcaa tcgacatgct gtgcgaaagc ggcgacatca gcgaacagga gaggtattgc
    29581 gaaacaccgt ccagttcgcc aacggtaaag gtcatggtgc catacggcag ttgcagacca
    29641 attttttcac tcactttgcg cagcggccag agttgatccg ggccggggaa gataactgca
    29701 ctcagcatcc acggggtgat cacacacccc gtccactgac cttcgaacag cgtaaaatca
    29761 gaaacataca ccggcattga aggatgcaga aaagagagat cgtgcatcga acgccgggca
    29821 atttcttcaa acgctgcctg tacttgcgcc ttcggggagg tctggaaacc tgctatctct
    29881 tcagtcatga atcgcctccc gtgggatggc ttccacgcct gattcgcgca gcgcagccag
    29941 aacctgctca agcgcaggtt caatcattgc ttcaaccgtc ggcgttaagc cgatgtgtgg
    30001 ctccagcgat tccgggatca cgccgaccag ggtcagcttt ttcggaaact caccggtgaa
    30061 gcgcagggcc gacaatacgt cggccaggcc aagctgatgc ggagagattt tgttggtaaa
    30121 caacgccgga acttcttcat cccgcaggat catcatcgtt cccggagtgt tctttttcga
    30181 cacaatggca tccgcaataa tcagatgatc gcgatttgcc atgtcgccaa gcagctccat
    30241 tcccgccgtg ccgccatcga ggatctcaac ataatccggc agaatgtatc gttgctctaa
    30301 cgcttcgaca atccgcacgc cgatggcttc atcggtcagc aaaatattgc cgacccctaa
    30361 gactaaaata cgcattacag aaccttcact gaaaccactt cgttgccgtc ggcatccact
    30421 acgtgtaccg cacaggccat gcacgggtcg aaggagtgaa tggtacgcac cacttccagc
    30481 ggtttattcg gatcggcaac cggtgtaccc accagcgact gctcgtaagg accgacgtca
    30541 tcattgaagt tacgcggacc agagttccag gttgatggaa caaccgcctg gtagttgctg
    30601 atgataccgt ctttaataac catccagtga gagagcatac cgcgcggcgc ttcgaggaag
    30661 ccaacacctt tgaactcacc cgttgccgga atgttcggct tcacaaaggt ggtgtgatcg
    30721 cctttgccga tattggtgat cagtgcactg tattggtttt gcaggatatc ctgcaattca
    30781 cagcagtgaa cggtacgacc aataatacgg cccagcgtgg agtgcagctg tgccacttcc
    30841 agcgtgttgc cagtcagttt ctgataaatc gcaacgattt cattcagttt gttctgggta
    30901 gattcgcgac ctgccgccag tttcaccagc atattagcca gcggacccac ttctaccgtt
    30961 ttgccgtaga aagtcggtga tttcacccag gaatatttcc cgtcgtcaga ccaaccatca
    31021 taagccggaa tggtggtacc ttcccacggc gcctgtggcg cttcgtcttt ataccaggag
    31081 tgcttcgcgc tttcctgaat gcctttgatc aggtattcat cggaatgaga agtgatcgga
    31141 cgatacgagg acagatccgc attctcaatg tagcctcccg ggaacaggaa gctgccgttt
    31201 ttgctgtcgg tcgggaattc cggcacgctc aggtagttca ccgcaccttt accgcgcgtc
    31261 agccattccg ggtagaacgc ggcgataacc gcggtatcaa ccttataaac ctgctcaaca
    31321 aagtcgctca gtttgtcgat gaaagacttg atgtacatca ggcgctcaag gttcaacacg
    31381 cccaacccgt cgaggttgat tgggttcgcg acaccaccca ctgccaggtt ctgaatgtgc
    31441 ggcgttttac cgcccagcag cgccacgacg cggttagcgt cacgctggca ctccagcgcc
    31501 tgcaggtagt gcgctaccgc aatcaggttc acttccggcg gcagtttcat cgccgggtga
    31561 ccccagtagc cattggcgaa aatacccaac tgaccgctgg caaccagatc tttgatcttg
    31621 ttctgaactt tagtgaactc ttccggactg ttcagatgcc aggtcgaaac gcctttcagc
    31681 atttcggagg ctttggttgg gtcagcttgc agtgcagaag tgatgtccac ccagtccagc
    31741 gccgaaagct gatagaaatg aacgatatgg tcatgcgtgg tgtgcgcagc cagaatgatg
    31801 ttacggatgt attgcgcgtt aaccggaacg tcgatattca gcgcactttc tgccgcacga
    31861 acggaagaca gcgcgtgagt ggtagtacat acgccacaga tacgttgcac aatcatccat
    31921 gcatcgcgcg gatcgcggtt tttcacgatc tcttccatgc cgcgccacat ggtaccggaa
    31981 gcccatgctt tcgaaacgac gccattttcg atttcgcaat cgatgcgtaa atgcccctca
    32041 atacgggtta ccggatcaat agtaattctc tggctcatgc tttgctcgcc tcatgacgat
    32101 tatgatcgtt ttgttttaaa ggaggaagta tcggcagtag acgaatgagt acgatgtaag
    32161 cgcaaatctc aatagccaca aaaccaatag aaatcaacag ttcttcccag gtcgggaagt
    32221 aggcgtaacc gccgcccggg ttgaatgcca ccaacgaata ggtcagacgc caggttgcac
    32281 aacctaacag tgcgctcagt gctgacagga acagcatgcg ggaatcatta cgcagcttcg
    32341 ccacacgcag aacgaccagc gggaagagca tcagcaggac ttcaatccag aacatcacgg
    32401 agtagaagtc accggcaaac gctaacgaca gcttgtcgcg atagataagc tcgccaaagc
    32461 gcagcccgat gaaaatcgcc agcaacacac tgatggtgtt ggtcagcttg acgaacaggc
    32521 tcttttcatc cggaccgttg ccacgcagac ccgcctgcac cagtgaacct tcaaagatga
    32581 caatcgagaa gcccatgatg aacgccgtca gcagcgagaa cagcggcaac atttcatagc
    32641 tctgccagag cggatgcacc ttgtagcccg ccgagatcat cagcgacccc attgaagact
    32701 ggtgcatggt cggcagcagc gcaccgagcg cgatgatgaa gaacatcacc ttgttcagac
    32761 gttgtagcga caccttccag cccagacgtt caaacagtgc cggagcaaac tccagagcca
    32821 tcacgccgat gtagatggtc atacagaccg ccgtctcgaa cagtaccgag ttcacgttga
    32881 agtgacccgg aatgtagaag tacggcaggt tccagtagcg acccacgtcg atagtgatcg
    32941 acaagccacc cagtgagtaa ccaaacagac tcgccaacag cgccggacgc accagcggat
    33001 ggtattgccc acggttaaag acgtataccg cccacgccag cgcccagccg ccacaggcaa
    33061 agccggtgcc aatcagcagg tcaaacgcga tccacacgcc ccacgggaag ccgccgttca
    33121 ggtcagagac agagcccaga ccgaacacca gacgcttcac aatcaggagc atacagatga
    33181 cgattaacgg tccaaaaatc atgaccggtt tactgatgat tttgccgccc agcggttgtg
    33241 gatcatgact catgatcgtc tcctccgtcg tgatggtcgt ttttggtgtt gcgacgaacc
    33301 agcacggtta agcccgccag cacagccagt ggtagcatca tgcctttata cagggtgtgt
    33361 tgaatatttt cggaacgcgc accggtagaa agatcgtcca gtttcggcag gtcgagattt
    33421 tcataaggca cacccgtcag taccagaacc tgagtaccgc cgccctcttt ctcgccgtac
    33481 agatgcggat aatatttcgg caccgtatgc aggtaagtgt cgccagattt cagcgtctga
    33541 cgcggatagt ggtattcgct gccaggcttc agcgccagac gttttttcgc ctccgccatc
    33601 agctcttcac gcgtaccgaa aatcaccgcg cccgccgggc acacttctac gcagccaggc
    33661 agaccgcctt tatcgagacg ttccacacct ttctggttgc acagctcgca cttatgcagc
    33721 gcaccaaacg ggttgttgta gtcgtacttc ggcacgttgt acggacaggc gaccatgcag
    33781 taacggcaac cggtgcacac gtctttgtcg taatggacaa tgccggtttt cggatctttt
    33841 ttcagtgcag agaccgggca cacagagaca cagttcggat cgacgcagtg catacactgt
    33901 ttcttaatgt acgcgtagcc gttctcctcc tggtctttgt tgacccctgt gccgctggtc
    33961 cacacctgaa tgatgttatt ggtatacggc gacagtttgt cgttgttcga ccaggtctgt
    34021 tccccttgcg ggttacgttc agggaagttg atatcctgac acttggtgac gcaagcctgg
    34081 cagcctacgc acaaggtcga gtcgtacaac atccccagcg atcccggaat tggcgggcgg
    34141 ttttcagcag ccgcatgact gacagacggc agcgcgcccg tcagcaatgc cccgcaggag
    34201 gctgctttaa taaaattacg tctgttcacg gttattctcc ccgtgagtca gcgttatctt
    34261 tcttttgctg acgacccagt tcacgcaccg ccatcacgct gacaccggca accagcccaa
    34321 ccacaccgcc gagcaaacca atagcgcctg cagagacgtt gccgccctct ttagcgttaa
    34381 catccggttt ctgtgaacgc ggagtctgat tttcgacgtt ggcaagctga tggatgcctt
    34441 tatggaagcc gatacctttt tcgttacagc catagcacgg gtgaccaatc gccaccggcc
    34501 acacaccgcc aacatcgcag aattgcagcg ttgagcagtt gccgtaagtt tctggccctt
    34561 tacagccgag gtggtacagg caccagcctt cgcggtggcc ttcatcaccg aactctttgg
    34621 caaaacgacc agcatcgaag tgcgggcgac gttcgcagtg ttcgtgaatc agacggccat
    34681 aggcgaaggt cggacggttt ttgtcatcca gtttcggcgg tttgccgtaa gtgatgatgt
    34741 gcgcaacggt cgcgaggaag ttgtgcgggt tcggcgggca gcccggaata ttgataacgg
    34801 ttttgcctgg cagaacttct tgcaagctga ctgcgccagt tgggttaact ccagctgcgg
    34861 caacaccgcc ccacgcggag caggaaccaa tagcgataat ggctgctgag ccttccgccg
    34921 ctttgcggat gtgatccaca atcggctcac cggcaaccat gcaataaata ccgttatctt
    34981 ttaatgggat ggaaccatcc accactaaca catactgccc tttgtacttc tcgagagcgt
    35041 tatgtttgtt ctcttcgacc tgatgaccga aggcggcgga aagcacttcg tgatattcca
    35101 gagagatagt ctccagcacg aggttttcta ccgttggatg cgttgcacga agcagagatt
    35161 ccgtacaacc ggtgcactcc tgcgcgccaa tccagataac tggcggacgc tgcgggttag
    35221 taaccgattc ggccatctct gcagcggctt tgctacttaa ccccatggtg gcggctaatg
    35281 ctgcacaaag cttcatgaaa tcacgacggt taatgccgtg agaatggatg agggtgttat
    35341 ctccagtcat ttatagttat tccgttgcga ggacctggca tatattttgc ctcaatcgca
    35401 aaatcaataa tgcgatcgat gcgccattta ccacacattt attatggtta tcgatatcat
    35461 gatactgcgg cggggcgcta aaacgaaggg aagaagcatt agtgtaatta attaaagcaa
    35521 gataatacgt atgtttgatc aattttcgag gcaataaccg gccataaaaa agcgcaacgt
    35581 agaacaggaa ttattctcac gaaagaaatc cattaccatc tctttatcca gtccatagcg
    35641 gcgcgtcaga atacccgctt cccaggcgct aagctggcga tctccggtct tttcgtacat
    35701 ccggtgtgag tagcctaata caaaaccgcg tttataatcg gcacagaatt ttgtcacatt
    35761 aaccgcactg tcggcatgcg ttgcttttaa tcccgccatt aagcctttac caaaatggtt
    35821 attcatcgtt cacctcattc gttatatatc gccatatata gcgatatttt aggcaaagaa
    35881 cgacttttgc gcacgctatt ttttcatact tgtttaagac gtaatcccgt aagagataaa
    35941 cctatcagga gagatattta tcgaaccatt tcagtgtcct ttgccaggca agatcggcgg
    36001 cagatttgtc ataacggggc gtggaatcat tatggaatcc gtgattaacc cccggataga
    36061 tataagcctc ataaacctta ttattgtctt tcaacgccgc ctcgtaagga ggccagccct
    36121 cgttgattcg ggtgtccagt tccgcgaagt ggagtagtaa aggcgcttca atcttcgcca
    36181 catcggcagt gggtgcctga cgaccataaa acggcaccgc gcaggccagt tccggatacg
    36241 cgacagccgc cgcgttcgat acgccaccgc catagcaaaa tccggtaata cccactttac
    36301 ctgtcgcttg cggatagcgt tgcataaact caattgcggc aaagaaatca ttcatcagtt
    36361 tggttggatc aacctgctgt tgcagctcac gacctttatc atcatttccc ggataacctc
    36421 caacggaact taatccgtca ggtgccaggg cgatataccc cgccttcgcc actcgccgtg
    36481 ccacatcttc gatatacgga ttcagtccac gattctcatg caccaccccc acggctggcg
    36541 ttttgccgct catttttgcg ggtttcacca gataaccccg tacctcgccg tgaccatttg
    36601 gcgaaggata cgtgatgtac tcagcaacaa tctccgggtc ggtaaactct acctgagtcg
    36661 ccagcgcata atttggcttg agcaaatcaa acaacgctaa tgccgtcatc ccgcccaccg
    36721 catacttcgc cgcaaggttg aggaactcac gtttcgaaat tttcccgtga acgtaatagt
    36781 cgtagtaatc caacaactct tgtgggaaat ctttggcggt taaacgcggc atcgttgcat
    36841 tcctcaactg gtgttttttt aagcaaagca taagcgcgtg tttttgccca gtttttcgtc
    36901 actctgtgag ccagactacg ggatacgcgc tggcgaatcg ctaaactaga aacattgttt
    36961 cgaaattgaa cggaggaaag gagaggtcat ggtctggtta gcgaatcccg aacgttacgg
    37021 gcagatgcag taccgctatt gcggaaaaag tggtttacgc ctgcccgcat tatcgctcgg
    37081 tttatggcac aatttcggtc acgttaacgc gctggaatca cagcgtgcaa tcctgcgtaa
    37141 agcgtttgat ttaggcatta cgcactttga tttagccaac aattacgggc cgcctccagg
    37201 aagcgcagaa gagaactttg gtcgcctgct gcgcgaggat tttgctgctt atcgcgatga
    37261 actgattatc tctaccaagg ctggctacga tatgtggccc ggcccgtacg gctctggcgg
    37321 ttcacgtaaa tacctgctcg ccagcctcga ccaaagcctg aagcgtatgg ggcttgagta
    37381 tgtcgatatc ttttactctc atcgcgtcga tgaaaatacg ccgatggaag aaaccgcctc
    37441 tgcgctggct catgcggtac aaagcggtaa ggcgctgtat gtcgggatct cctcttactc
    37501 gccagagcgg acgcaaaaaa tggtcgagtt gctgcacgag tggaaaattc cgcttttaat
    37561 tcatcaacct tcgtacaatt tactgaaccg ctgggtggat aaaagcggcc tgctggatac
    37621 cctgcaaaat aacggcgtgg gctgtattgc ctttactcct ctggctcagg gattgctgac
    37681 cggaaaatat ctcaacggca ttccgcaaga ttcacggatg catcgtgaag ggaataaagt
    37741 tcgtggtctg acgccgaaaa tgcttaccga agccaacctc aacagcctgc gcttattgaa
    37801 tgaaatggca cagcagcgtg gacaatcaat ggcgcaaatg gcgttaagct ggttgctgaa
    37861 agatgatcgc gtgacgtcgg tattgattgg tgccagccgc gcggagcaac tagaggagaa
    37921 cgtgcaggcg ctgaataatc tgacatttag caccgaggag ctggcgcaga ttgatcagca
    37981 tatcgccgat ggcgagctga atctgtggca ggcgtcgtcc gataaatgac ctgttaataa
    38041 cgggcgcgcg aaccgcgccc gaatcagatc agtgattatg acgagtcagt cggtcaagat
    38101 agcccatcac aaatgcagaa agcacaaacg tcagatggat aatgacgtac cacatcagtt
    38161 tgttatccgg gacatttttc gcatccataa agacgcgcag taagtgaatg gaagaaattg
    38221 ccacaatcga cgctgctact ttgtttttca gcgacgttgc gtccattttc cccagccact
    38281 tcagcttctc tttgttctcg gagatatcca gctgtgagac gaaattctca taaccggaaa
    38341 acatcaccat caccagcaaa ccgccaacca gcgtcatatc caccagcgac agcaacacga
    38401 ggatcaaatc tgattccgcc atcgagaaga tattcggcag tacgtgaata atctcctgga
    38461 agaacttcag cgccagggca actaacgcca gcgaaaggcc aaagtacacg ggggcaagca
    38521 gccagcgaga agcatacatt gcattttcaa gaaaacgttc catacattcc tgtcgtgtag
    38581 ttaaaacggg caacagtata tcgcaattga gtaaacagca cgcaacagca aatcaacata
    38641 tacgttaaac ctttgtcgca taattctgag gtttagggat cttcctggtt cccttatcaa
    38701 cgcatcgaga gcgtctaccc ttaaaggacg ttgaacctga agggagaaaa acgatgtctc
    38761 atttaaaaga cccgaccacg cagtattaca ctggtgaata tcccaaacag aaacaaccga
    38821 cgccaggcat ccaggcgaag atgacaccga taccggattg cggcgagaaa acctatgttg
    38881 gtagcggtcg cctgaaagat cgtaaagcac tggtgacagg gggcgattcc ggaataggtc
    38941 gcgctgccgc catcgcttac gcgcgtgaag gggctgacgt ggcgatcagt tatcttcccg
    39001 tggaagaaga agacgctcag gatgtgaaaa agatcattga agaatgcgga cgcaaagccg
    39061 ttctgctgcc aggcgattta agcgatgaga aattcgcccg ttcgctggtt cacgaagcgc
    39121 acaaggcgtt aggcgggctg gatattatgg cgctggtcgc cgggaaacag gttgccattc
    39181 cggatattgc agacctcacc agcgaacagt ttcaaaagac ctttgccatt aacgttttcg
    39241 cgctgttctg gctaacccag gaagcgatcc ccctgctacc gaaaggtgca agtgtcatca
    39301 ccacttcgtc aatccaggca taccagccaa gtccgcattt actggactat gcggctacga
    39361 aggcggcgat tctgaactac agccgtggcc tggcaaaaca ggtcgcggag aaaggtattc
    39421 gggtgaatat tgtcgcgcca ggccctatct ggacggcact gcaaatttcc ggcggacaaa
    39481 cgcaggataa gatcccgcag tttggtcaga aaacgccgat gaagcgtgcg gggcaaccgg
    39541 cggaactggc ccctgtatat gtttatctgg caagtcagga gtcgagctac gtcaccgcag
    39601 aagtgcacgg cgtgtgcggc ggcgagcatt taggttaaaa aaatgcccgg ttgtgaaaag
    39661 caaccgggca tcattgtgaa ttcactctta cctgatgtgg cgaaaacgcc ttatccgcct
    39721 acaaacgcat gaagattcaa catgttgcaa tagattttgt aggcctgata agcgtagcgc
    39781 atcaggcatt gagcacctaa tgcctgatgc gtcgtgaacg ccttatccgg cctacaaacg
    39841 cacgaagatt caacatgttg caatagattt tgtaggcctg atgagcgtag cgcatcaggc
    39901 aattctactt tacttcgctt tggcggtttc ttcgccgacc agacctatct tcaggtaacc
    39961 cgcctgatgc agcgtatcca ttaccttcat caacgtctcg taatcgacgg ttttgtccgc
    40021 tcggaagaag atggtggtgt ctttcttgcc ttcggttaac gcattcaacg ccgtaatcat
    40081 tgtttcatcg gtgaccggat cgttaccgat aaacatcgag ttgtctgcct tcaccgacag
    40141 ataaaccggt ttttccgggc gcggctgcgg cgtgctggta gaagcaggca agttcacctt
    40201 cacatctacc gtcgctaacg gtgccgccac cataaagata atcagcagaa ccaacatcac
    40261 gtcgataaac ggcgtcacgt tgatatcatg catttcgccg ttatcgtcga ggttttcgtt
    40321 aagatgcatt gccatcggac attatcctgc gcgtaatttt tgtgcgacac gaaccggatg
    40381 cgcagcggcg ctggcttcca gatccaggtc acggctttgc agcaacaata cctgcgctgc
    40441 aacatcaccc agcatcgctt taaagccgcc aatctggcgt gcaaatacgt tatagataac
    40501 gaccgcagga atcgctgcca cgaggccgat tgccgttgct aacagagctt ctgcgatacc
    40561 cggcgcaacg actgccaggt tagtggtctg cgtttgcgcg ataccaataa agctgttcat
    40621 gatgccccag accgtaccaa acagaccaac gaacggagaa atcgcgccga tggttgccag
    40681 atagccgtta ccgcggccca tttgacgacc cactgcggcg acccgacgct ccagacggaa
    40741 gctggtacgt tctttaatac cttcgttatc gtcgctgcct tctgacagtt ccagctcatt
    40801 ctgcgcttca ttgagcaaat gcaggcttaa gcttttgcta ccaaaatcag cggcgatatc
    40861 gttggcctgg tttaaggaac gtgcttcagc cagcagttgc tgctcgcgct taaggcgacg
    40921 cttctgattg aagaactcta cgctcttact gaagaagatt gcccaggtga ctacagaggc
    40981 caaaataagc ccaatcatca cgcacttaac gacaatatcg gcgtactgat acatacccca
    41041 gacggaaagg tccgtctgca ttaaattatt acccactgtg tatctccagg acgcaagtca
    41101 caaaatctgc gcataataat atcaaaacga cgtcgaattg atagtcgttc tcattactat
    41161 ttgcatactg ccgtaccttt gctttctttt ccttgcgttt acgcagtaaa aaagtcacca
    41221 gcacgccatt tgcgaaaatt ttctgcttta tgccaattct tcaggatgcg cccgcgaata
    41281 ttcatgctag tttagacatc cagacgtata aaaacaggaa tcccgacatg gcggacaaaa
    41341 agcttgatac tcaactggtg aatgcaggac gcagcaaaaa atacactctt ggcgcggtaa
    41401 atagcgtgat tcagcgcgct tcttcgctgg tatttgagag tatggaagcc aaaaagcacg
    41461 cgacgcgcaa tcgcgccaat ggcgagttgt tctatggacg gcgcggaacg ttaacccatt
    41521 tttccttaca acaagcgatg tgtgaactgg aaggtggcgc aggctgcgcg ctatttccct
    41581 gcggggcggc agcggttgct aattcgattc ttgcttttgt cgaacagggc gatcatgtgt
    41641 tgatgaccaa caccgcctat gaaccgagtc aggatttctg tagcaaaatc ctcagcaaac
    41701 tgggcgtaac gacatcgtgg tttgatccgc tgattggtgc cgatatcgtt aagcatctgc
    41761 agccaaacac taaaatcgtg tttctggaat cgccgggttc catcactatg gaagtccacg
    41821 acgttccggc gattgttgcc gccgtgcgca gtgtggtgcc ggatgccatc attatgatcg
    41881 acaacacctg ggcagccggt gtgctgttta aggcgctgga ttttggcatc gatgtttcta
    41941 ttcaggccgc caccaaatat ctggttgggc attcagatgc gatgattggc actgccgtgt
    42001 gcaatgcccg ttgctgggag cagctacggg aaaatgccta tctgatgggc cagatggtcg
    42061 atgccgatac cgcctatata accagccgtg gcctgcgcac attaggtgtg cgtttgcgtc
    42121 aacatcatga aagcagtctg aaagtggccg aatggctggc agaacatccg caagttgcgc
    42181 gagttaacca ccctgccctg cctggcagta aaggacacga attctggaaa cgagacttta
    42241 caggcagcag cgggctattt tcctttgtgc ttaagaaaaa actcaatgat gaagagctgg
    42301 cgaactatct ggataacttc agtttattca gcatggccta ctcgtggggc gggtatgaat
    42361 cgttgatcct ggcaaatcaa ccagaacata tcgccgccat tcgcccacaa ggcgagatcg
    42421 attttagcgg gaccttgatt cgcctgcata ttggtctgga agatgtcgac gatctgattg
    42481 ccgatctgga cgccggtttt gcacggattg tataacattg ccacttttgg acaattttgc
    42541 agacatttta ttgtgaaaag tcttaaattg ttgcgtccgg gatcaaggcg tcccggacga
    42601 ttcaggagta caataggcag ataaaggctt aaacgctgtt ccacaggaaa gtccatggct
    42661 gttattcaag atatcatcgc tgcgctctgg caacacgact ttgccgcgct ggcggatcct
    42721 catattgtta gcgttgttta ctttgtcatg tttgccacgc tgtttttaga aaacggcctg
    42781 ctgcccgcct catttttgcc aggcgacagc ttgttgatac tggcaggcgc attgattgcc
    42841 caaggggtta tggattttct gcctacgatt gcgattctga ccgccgcagc aagtctgggc
    42901 tgctggctaa gttatattca ggggagctgg ttagggaata ccaaaacggt gaaaggctgg
    42961 ctggcacagc ttcctgctaa atatcaccag cgcgccacct gcatgtttga ccgccacggt
    43021 ctgctggcgc tgctggctgg acgttttctt gcatttgtcc gtacgctgct gccaaccatg
    43081 gcgggaattt ccggtctgcc aaaccgccgc ttccagtttt tcaactggtt aagtggattg
    43141 ctgtgggtca gcgtggtaac cagttttggc tatgccttaa gtatgattcc gttcgttaaa
    43201 cgccatgaag atcaggtaat gacgttcctg atgatcctgc caattgcctt gttaaccgct
    43261 ggcttgttag gcacgctgtt tgtggtgatt aaaaaaaaat actgtaacgc ctgacgattt
    43321 tccccgttcc cggctgctgt accgggaacg tatttaattc ccctgcatcg cccgcattct
    43381 tgccgcatct tcccccggcg tcacaccgaa gtaacgttta aactcacggc taaattgcga
    43441 tgcgctttca tagccgacgc gcatcgctgc tgcgctggcc ttcatgccgt catggatgat
    43501 catcatccgc gccttatgca gacggtaatt cttcaaatac tgcaacggcg aggtgctggt
    43561 gacagactta aaattatggt ggaacgccga tacgctcatg ttggcttctg ccgccagttg
    43621 ctcgacgctc aggttttcgg tgtatttatt ctcaattcgt ttcagcacgc ggctaatcag
    43681 actgaagtga gtctggcgac tgaccagcgc cagtaacgcg ccgccgcaag gtccggtcag
    43741 cacgtagtac agaatttcgc ggatgatctg tttgccgaga atacgcgcat ccagtggtcg
    43801 ctccatcacg tcgagtaacc gctccgccgc gcataaaatc tcttctgata acgtggcgga
    43861 gttaatcccg ctggctgcca tcgacggctg gaaatgctca tcttcgccaa tgtccatcaa
    43921 cagttcctgt aactgcaaaa tatcgacatt gagacgcaac cctgccagcg gcacctctga
    43981 cgtggcatag gtttcgcact caaacggcaa cggcaccgtc agcagcaggt attcattggc
    44041 atcataacga aacacgcgtt cattgatata accgatttta tgcccggaaa agagaattat
    44101 gatgccaggc tcgtacatca ccggtgtacg tgcgaaaggc gtctcgccat acaacaaacg
    44161 cacatcgggc aacagttctg acaaactatt ttctttattt ttcagtttat taactttatc
    44221 cgccagcaag cgacaaatct cttcacgttt catatcgcgt aatttcttag gaataatgcg
    44281 gcaatttgat tgtgcgcaat tttgtagcat ttctccagca ctctggagaa ataggcaaga
    44341 cattggcaga aatgagcatt gagagccagg gcgctggcga tcacaatgaa aaacatcagg
    44401 cagatcgttc tctgccctca tattggccca gcaaagggag caagtaatga acaactttaa
    44461 tctgcacacc ccaacccgca ttctgtttgg taaaggcgca atcgctggtt tacgcgaaca
    44521 aattcctcac gatgctcgcg tattgattac ctacggtggc ggcagcgtga aaaaaaccgg
    44581 cgttctcgat caagttctgg atgccctgaa aggcatggac gtgctggaat ttggcggtat
    44641 tgagccaaac ccggcttatg aaacgctgat gaacgccgtg aaactggttc gcgaacagaa
    44701 agtgactttc ctgctggcgg ttggcggcgg ttctgtactg gacggcacca aatttatcgc
    44761 cgcagcggct aactatccgg aaaatatcga tccgtggcac attctgcaaa cgggcggtaa
    44821 agagattaaa agcgccatcc cgatgggctg tgtgctgacg ctgccagcaa ccggttcaga
    44881 atccaacgca ggcgcggtga tctcccgtaa aaccacaggc gacaagcagg cgttccattc
    44941 tgcacatgtt cagccggtat ttgccgtgct cgatccggtt tatacctaca ccctgccgcc
    45001 gcgtcaggtg gctaacggcg tagtggacgc ctttgtacac accgtggaac agtatgttac
    45061 caaaccggtt gatgccaaaa ttcaggaccg tttcgcagaa ggcattttgc tgacgctaat
    45121 cgaagatggt ccgaaagccc tgaaagagcc agaaaactac gatgtgcgcg ccaacgtcat
    45181 gtgggcggcg actcaggcgc tgaacggttt gattggcact ggcgtaccgc aggactgggc
    45241 aacgcatatg ctgggccacg aactgactgc gatgcacggt ctggatcacg cgcaaacact
    45301 ggctatcgtc ctgcctgcac tgtggaatga aaaacgcgag accaagcgcg ctaagctgct
    45361 gcaatatgct gaacgcgtct ggaacatcac tgaaggttcc gacgatgagc gtattgacgc
    45421 cgcgattgcc gcaacccgca atttctttga gcaattaggc gtgccgaccc acctctccga
    45481 ctacggtctg gacggcagct ccatcccggc tttgctgaaa aaactggaag agcacggcat
    45541 gacccaactg ggcgaaaatc atgacattac gttggatgtc agccgccgta tatacgaagc
    45601 cgcccgctaa gctttttacg cctcaaactt tcgttttcgg gcatttcgtc cagacttaag
    45661 ttcacaacac ctcaccggag cctgctccgg tgagttcata taaaggagga acgtatggct
    45721 aatccaaccg ttattaagct acaggatggc aatgtcatgc cccagctggg actgggcgtc
    45781 tggcaagcaa gtaatgagga agtaatcacc gccattcaaa aagcgttaga agtgggttat
    45841 cgctcgattg ataccgccgc ggcctacaag aacgaagaag gtgtcggcaa agccctgaaa
    45901 aatgcctcag tcaacagaga agaactgttc atcaccacta agctgtggaa cgacgaccac
    45961 aagcgccccc gcgaagccct gctcgacagc ctgaaaaaac tccagcttga ttatatcgac
    46021 ctctacttaa tgcactggcc cgttcccgct atcgaccatt atgtcgaagc atggaaaggc
    46081 atgatcgaat tgcaaaaaga gggattaatc aaaagcatcg gcgtgtgcaa cttccagatc
    46141 catcacctgc aacgcctgat tgatgaaact ggcgtgacgc ctgtgataaa ccagatcgaa
    46201 cttcatccgc tgatgcaaca acgccagcta cacgcctgga acgcgacaca caaaatccag
    46261 accgaatcct ggagcccatt agcgcaagga gggaaaggcg ttttcgatca gaaagtcatt
    46321 cgcgatctgg cagataaata cggcaaaacc ccggcgcaga ttgttatccg ctggcatctg
    46381 gatagcggcc tggtggtgat cccgaaatcg gtcacacctt cacgtattgc cgaaaacttt
    46441 gatgtctggg atttccgtct cgacaaagac gaactcggca aaattgcaaa actcgatcag
    46501 ggcaagcgtc tcggtcccga tcctgaccag ttcggcggct aacgtgcaaa ttctcccggt
    46561 ggcggtaatg ttccgctacc ggacttttca gaaatcattt attcccctcg cgtcccgccc
    46621 gttgttactc ttccttgttc aggaatgcca aatataagga catcatcatg cagagccgga
    46681 agctcttaaa agaacaactc atctatatcc gggataaacg caacggagag gtgaaaaaca
    46741 gatgaaaata atacttctgt ttttagcagc cctggcaagt tttaccgtac acgcgcagcc
    46801 tccttcacag accgtagaac aaacagtccg gcagatttat cagaactata aatcagatgc
    46861 cagtacgcct tatttcggtg aaaccggaga gcgggcgata acttcagcgc gtattcaaca
    46921 ggcgcttacc ctgaacgaca atcttacact accgggcaat attggctggc tggattatga
    46981 tccggtttgt gattgtcagg attttggcga tctggtgtta gaaagcgtag cgataaccca
    47041 aactgacgtc gatcatgccg atgccgttgt gcgctttcgt atctttaaag atgataaaga
    47101 aaagaccaca cagacactga aaatggtggc ggaaaatggt cgttgggtca ttgatgatat
    47161 tgtcagcaat catggcagcg tcttacaagc agttaatagc gagaatgaaa aaacgctggc
    47221 cgctttagct tcattgcaga aagaacagcc agaatccttt gttgctgaac tctttgaaca
    47281 tattgctgat tatagctggc cgtggacgtg ggtggtttcc gactcttacc gccaggcggt
    47341 taatgccttc tataaaacca ccttcaagac ggccaataat cccgatgaag atatgcaaat
    47401 agaacggcaa tttatttacg acaatccgat ctgttttggc gaagagtcgc tattttcacg
    47461 cgttgatgaa attcgagtcc tggagaaaac cgccgattcc gcccgcattc atgttcgttt
    47521 tacgctgacc aatggcaaca atgaagagca agagctggtt ttacagcggc gcgaaggtaa
    47581 gtgggaaatc gctgatttta tccgcccgaa cagcggcagc ctacttaagc agattgaggc
    47641 aaaaaccgcc gccagattaa agcaatgagc tgaattaaat aacaattagc cggaacaata
    47701 aataaaaggg aacactatat gaaaacgatt ttcaccgtgg gagctgttgt tctggcaacc
    47761 tgcttgctca gtggctgcgt caatgagcaa aaggtcaatc aactggcgag caatgtacaa
    47821 acattaaatg ccaaaatcgc ccggcttgag caggatatga aagcactacg cccacaaatc
    47881 tatgctgcca aatccgaagc taacagagcc aatacgcgtc ttgatgcaca ggactatttt
    47941 gattgcctgc gctgcttgcg tatgtacgca gaatgataaa aaaatccccg gcagcatgtc
    48001 agttgccggg gatttttttt aacgtccaac cgccgcttta gggcgtttct tcgcaccagc
    48061 attcaccgga cgagattgcg tagacgacgc tttttttgcc gtagcaggcg tctgacgctg
    48121 agtcgccatc ggcgtatgtt tagtcaacgc cggacgggta ttgcggttct ggcgacgagc
    48181 ttcacgcatc tcttcaatgg ttggcgcagg cactaagcaa tcgcgacggc tgccaatcag
    48241 atgctttttg cccatcgctt ccagcgcctg acggattaac ggccagtttg ccggatcgtg
    48301 gtaacgcaac aacgctttat gcaaacgacg ctgtttgtcg cccttcggta cgaagacgcc
    48361 ttcgctctta taaccaatct tcgccagcgg ttttttcccg gtgtaataca tggtggttga
    48421 gttcgccagc ggcgatgggt agaagttctg tacctggtcg agacggaaac gatgcttttt
    48481 cagccacagc gccaaattca ccatatcttc atcacgcgta ccggggtgcg cagagatgaa
    48541 atacgggatc agatactgct ctttacctgc ctgtttcgag taggtatcga acagctcttt
    48601 aaagcggtca tagctgccca tgcccggctt catcatcttc gacaacggcc cttcttcggt
    48661 atgttccgga gcaatcttca gataaccgcc gacgtgatgg gtcgccaact ctttgatata
    48721 gcgcggatct tccacggcga tgtcataacg tacgcctgag gcgataagga tttttttaat
    48781 gcctttcaga tcacgcgcac ggcgatagag gttgatcgtc ggttcgtggt tagtgtccat
    48841 gtgcggacaa atatccggat aaacgcacga caaacggcga caagtttgtt cagcgcgtgg
    48901 cgatttgcag cgcaacatat acatgttggc agttggccca ccgagatcgg aaatcacgcc
    48961 cgtaaaacct ggaacggtgt cgcggatcgc ttcgatctca ttaatgatcg aatcttccga
    49021 acggctctga ataatgcgcc cttcgtgctc ggtaatagaa cagaaagaac agccgccaaa
    49081 gcagccgcgc ataatgttga ccgaaaaacg gatcatttcg taagccggaa tacgggcatt
    49141 gccataggcc ggatgtggca cgcgcttgta cggcagcgca aaaacgctgt ccatctcttc
    49201 ggtagaaagc gggatagcag gcgggttgat ccacacatag cggtcaccgt gtttttgcat
    49261 caatgcgcgg gcgcagcctg ggttggtttc gtggtgcaga atgcgcgaag catgggcgta
    49321 cagcacttta tcgcccttca ctttctcgaa agaaggcagc aatacgtagg ttttttccca
    49381 cggtttctgg cgcggtggct gcacgattac agctttcgct tcctgctttt tcggcgcaac
    49441 aggtttgtta tccgcacacg gcaaatcttc gccatacgga tgcgggattg gggcgatttt
    49501 ccctggggta tcaagacggg tggaatccac accgctccag ccaggcagcg cctcttttac
    49561 gataattgcg gtattacgca catcgcggat ttcactaatc ggctcgccca tcgccagacg
    49621 atgcactact tccaccagcg gacgttcacc gttaccaaac atcagcatgt cggctttcga
    49681 atctaccaga acggaacggc gcacggtatc ggaccagtaa tcataatgcg cggtacggcg
    49741 caggctagcc tcaataccgc cgaggatcac cggcacatct ttccacgcct ctttacaacg
    49801 ctgggtataa accagcgtgg cgcgatccgg tcgcttaccc gcgacattat ctggcgtgta
    49861 ggcatcatca tgacgtaaac ggcgatcggc ggtatagcgg ttgatcatcg aatccatgtt
    49921 gccagcagta acaccaaaaa acagattcgg tttacccaga cgcataaagt cgtctttgct
    49981 gctccagtcc ggctgggcga tgatcccgac gcgaaagccc tgcgcttcca gcatacgacc
    50041 gcaaatcgcc atcccgaagc ttgggtgatc gacatacgcg tcgccagtaa ccaaaatgat
    50101 gtcgcagcta tcccagccaa gttgatccat ctcttcacga gacatcggca aaaacggtgc
    50161 cggtccaaaa caggcggccc agtactgcgg ccaggagaac aggtcgcgat ccggttggat
    50221 cagggagata gagctcattt tgcttccaga aatgataaaa aaataatcaa aggccgggga
    50281 ttataagccg gaacgaaaga gaaatcgaaa ggtattccat actcgccctc ctcgggcgag
    50341 tatgaagatt acgataccgg attgaccaac agttgcccaa tcgagccacg gtccgccatt
    50401 tccagcgtct gactgttgaa gtagaacggg aagtgcgccc aggaaggctg accgaaataa
    50461 acaagcagct ccacttgtcc gtcaacccaa acggtatctt tccagcctct gtcttccggg
    50521 aacggcatcg cgccattcac gttacggatc tggaacatta cgccttcaat atggaacgct
    50581 tgcggctcgt ccgcgcgtac cgtccagcgt tcccacgttc cttgctgcgc ggtgacatca
    50641 atacggttga cgtcccacag ctgtccatta atacccgggt catcacccag actgatatcg
    50701 cgactgcgaa ttggcgaacc agccatgatt tcagttggca gcaagcgcat cggaagactg
    50761 tctgtgacca gcggcagaag gccggttggg cgcagcgtta gcaccaaggt agaaaccaga
    50821 atactggatg gctcaaagaa gccgcgaata cgatcaacaa tgctcgccgc ttcaccacag
    50881 gtgatcgaca cttcatcgcc gttgctcata tccaccagaa tctcgcggcg ctcgcccggt
    50941 gccagcgaaa gttgcttcac cgatacagga gcaggcagga atccctgatc gccagaaatc
    51001 acatgtaacg ggcgaccatc gctcatctgt agttgatagc gacgagagtt cgacgcgttc
    51061 agcagtcgca agcgcaccca gccacgcgag acttcaacgt acgggctttg tacaccgtta
    51121 accagcagcg tatcaccaac aaagccgccg cttcccggtt cgttgtattc tggcgtacca
    51181 aagttatcca gccgtttatc ctggataatg accggaaaat catccacacc ataatggttg
    51241 gggataggca gcgacttgct gacttcatct tccaccagcc acattccggc aaggccgtta
    51301 tagacctgct gagccgtgcg gttgggagta ttggcgtgat accacagagt agctgcgttc
    51361 tggcgaatag gcagtacggg tgcccagtca gcgtttggcg acatcatccg tgccggaccg
    51421 cccatcagcg ggcctggtac ctgtagcccg gccaccgtca ttgagacatt ttctgtcagg
    51481 cggttgctgt aaataagctt aacatcgtcg cccttccaga cgcggatagt cggccccagg
    51541 taacgaccat tgattcccca gaccgacgcg cgtgtccctg gcgtaaatga ccagtgcgca
    51601 cgttgtacag tcataaacag cggttgccca cggcgagatt caagtagcgg cggaacgggt
    51661 agcggttgct gttgcccggc tgcgctggcc ttcaggggaa cagcgcctgc acaaagtgca
    51721 atccccgatg cctgaatgaa ctgacgccga ctgagtgaca tatttgctcc atgtaaaact
    51781 ggctaaagta ataccgtgca ggaaagtgtt ccccgcaaaa atacagattg ccgacttaaa
    51841 cttttccggc ggcttcgcgt tctgcgactt ctttatcgag ctcggcgatt ttttgttcca
    51901 ttatcgaacg acaatgggca gccagctcac gaacctgatc tttgccatac tgactgacgt
    51961 caattggcgg cagcatttcg acaatcacca gaccgttgtg cagtcgatta agattaatct
    52021 tattcgaagt tgtagagacg cacacgggaa taatcgggac gcccgccgca attgccgcgt
    52081 gaaatgctcc agtcttgaac ggtagcaggc cgcgaccacg gctgcgggtt ccttccggga
    52141 acatccagat ggaaatacgg cgttttttga aatgattcac gacttccgca atggtgccgt
    52201 gagctttagt gcgattgttt ctgtcgatca ataagttgcc ggttaaccag tacaactgcc
    52261 cgaagaaggg gatccacagc aagctctttt tacctaccgt caccgtcggc ggttgcacaa
    52321 tgttcgatgc tgtcaccatg tcatagttgt tctggtggtt agcgatatag atagcattgc
    52381 cgtagttttc agcatctgca ggtttgcggc actcaacttt caggccgaac agtggcgcaa
    52441 gacggccaaa catatgccca aaggtggcca catgtttcgg gttacgcggg ctgaaaaggc
    52501 agtaaatgga gccgaataca cagactaaga tgctgtaaat cacggtaata ataagacgaa
    52561 agatatatag cataacaccc tctgaaggtc tgagagactg gcattgtacg tcacctgaaa
    52621 tcaggttagg gcattgttaa aagccgctct ctggaaaagc gtattgttaa tcgcaacgca
    52681 tgaataaaca acggtttcaa gggaattttt atcgtttccg ggggttgaat acgctgccgg
    52741 atgacgactt aacgtttcat ccggcgttcc ttgctagcgg gaggaaacag cgccctcccc
    52801 ggcatattac tcttcgctat caccgctgct ggcacggcga ggagagtcga tctcaacacg
    52861 atcgatacgc tgcaaaccgc gcatcaacgt accgcggcgt ccacgttcgc cagtgacttt
    52921 ctgtagctct tccggacgca gtttaatttt gcgtttccca acatgaatgg tcagcgtgct
    52981 ttgcggcggc agaacgtaca gttgcgccag accatcctcg ccacgcgcgg cttctgccga
    53041 tggaatgttg ataatcttgt tgcctttgcc cttcgacagc tgcggcagat cacttaccgg
    53101 gaacatcaac atacggcctg cctgagtgat tgccagcagc atatcggaag catcttcaat
    53161 caccaccggc ggcataacat gggcattttc cggtaaggtg atcaaagcct tacccgcacg
    53221 gttacgcgct accagatcgt taaaggtgca gacgaaaccg taacccgcat cggaagccat
    53281 cagcagtttc tgatcgtcgc tttccatcag catatggtca acggtcgccc caggcggcaa
    53341 cgttaatttg ccggtgagtg gttcgccctg accacgcgcc gacggcagcg taatcgggtc
    53401 gatggcatag ctacgaccgg tggaatcaac aaacactacc ggttggttac tcttaccttt
    53461 caccgccgct ttgaagctat cacccgcttt ataattcagg cccggcgcgt cgatatcatg
    53521 gcctttagcg ctgcgtaccc agcccatctg cgacagcaca atggtgacag gttcagacgg
    53581 cagcatgtcg tgctcgctca tcgctttcgc ttcttcgcgt tcctgcaacg gcgaacgacg
    53641 atcgtcaccg taggcttgcg cgtctgcctg cagttctttc ttcagcaggt tattcatttt
    53701 acgctcggaa gccaaaatgc cctgcaactg gtcgcgctct ttttccagct cactctgctc
    53761 accgcgaatc ttcatctctt ccagtttggc aagatgacgc agtttcagtt cgaggatcgc
    53821 ttccgcctgg gtttccgtaa ggccaaaccg cgacatcagc gccggtttcg gttcatcttc
    53881 gttacgaatg atctcaatca cttcgtcgat gttgagaaac gccaccagca aaccttccag
    53941 gatatgcagg cgcttgagga ctttctccag acgatagttc agtcggcggc gcacggtatc
    54001 gcgacggaac accagccatt cggagaggat ttccagcagg tttttcaccg ccggacgacc
    54061 atccagaccg atcatattga ggttaatgcg atagctcttt tccagatcgg tggtagcgaa
    54121 gaggtggttc atcacctgat ccatatccac gcggttggaa cgcggcacaa tcaccagacg
    54181 ggtcgggttc tcgtggtcag attcatcgcg cagatcgtca accatcggca gctttttgtt
    54241 gcgcatttgc gcagcaattt gctccagtac gcgcgcacct gaaacctgat gcggtaatgc
    54301 gctgataacc accgcgccat cttctttctt ccacaccgcg cgcatacgca ctgaaccacg
    54361 tccgttctcg tagattttac gaatctcggc gcgcgaagtg ataatttccg cttcagtcgg
    54421 ataatccggc ccctgcacga tatccagcag ctgatcgagc gtagttttcg gctggtcgat
    54481 taatgcgatt gccgcctgag ctacttcacg caggttatgc ggtggaatat cggtcgccat
    54541 gccgacggca ataccggtgg tgccgttaag caaaatgttt ggcagacggg caggtagcat
    54601 tttcggctcc tgcaaagtgc cgtcgaagtt tggcacccag tcagccgtcc cctgccccag
    54661 ctcgctcaat agcagctcgg aatatttcga caaccgggat tcggtgtaac gcattgccgc
    54721 gaacgatttc ggatcgtccg gcgcgcccca gttcccctga ccatcaacca gcggataacg
    54781 gtaagagaac ggctgcgcca tcaggaccat cgcttcataa caggcgctat cgccgtgcgg
    54841 atggtattta cccagtacgt caccgacggt acgggccgat tttttaaatt tggcgctggc
    54901 attcaggccc agttcagaca tcgcatacac aatgcggcgc tgaacaggtt tcagaccatc
    54961 accaataaac ggcaacgcac ggtccatgat cacgtacatg gagtagttta agtaggcgtt
    55021 ttccgtaaat tcatgtagcg caaggcgctc tgccatatcg ctcattaatt ctgattcctc
    55081 aacttattcg ccagccttca aaggggcaat attgccggag atactacatt atctgccgcg
    55141 tcgagtcaca ggagatcagc gtgaatagcg ccgccggacg tgctccgacg gcgataaagg
    55201 catcaatgtg ccttgatata caactctttg ctgtagactt gatcggcata gagggcagca
    55261 caaggttgcg cccatgccac accactcggc atgggggtcc gctccgggcg attcgagaag
    55321 ttgaggttat gcgagtccag ggaagcctaa agcatcaaag tagtcgactg taagtatacg
    55381 gttcagccca gtttagctgt gtctccacca gtgccccgcg cctcctgcca taagtgcggc
    55441 ttgctcatgg atatccccga gacggcttac ggcgcaccgg ataataaatt tcgcgtatac
    55501 atggtattcc ttaccggaga aattataagc ccaacaacaa tacgcctgtg acattgttcg
    55561 ctcaaactta tcgcgagtga tttggctcac actccggtaa agattcccca tcacgccaac
    55621 ggcacataaa catccgtttc cagtcgctgc tctgtcaccc cttcggcaag attggtgtag
    55681 tgaaacagaa tcggtgcttt acgcattttc tcgccgcttg caggcagcca gtggcgaata
    55741 atgccccata tcgtgtgtga aatatcgtcc agctcgccaa cgtggcgggc cacggcataa
    55801 cgtccaccgg taagttcacc attgctcaca ccataacgat tatcgggaat cggttcgcta
    55861 acgctgccgc agatatcaaa gcgaaacgct tccggtgcgg tggtggcagg ggcgtcccag
    55921 gcgacgccaa aagtctggct ttggttaaca ggagaaagcc ccgtctcttt acgccacatg
    55981 ataaatttcg ccacactgta gttgagcaac tcagggctac agcggtgcgg caacatcgcc
    56041 acagggattg atgggaaatc gataatgttt acatccagtg tcaggtttgt cattttgcct
    56101 ccctgcgaaa ttattcccgg gaacgcgtcc cgggagcggt aacaattacg gatttacttt
    56161 gcggatctgt ttgacgtcaa tttcaacaga attccagtct ttatcgactt caccctgaat
    56221 ctcaaccgta tctttcggcg tcaccgtcac accgttccag cgtttgtgat cgatatcaac
    56281 attgatagta ccgctggcat ctttaaacac gtagagatcg tcagagatgc gttcaacgat
    56341 attgccgcgc agggttaccc aggtgtcgtc acgcagggat tttgcgcttt ctacagtcgt
    56401 tacgctgccg ttcggcccct gaaatcctcc ggcctgactt tgcgttaccg atgggccaga
    56461 aaaaccgccc tgctctgctg ccatcaccgg tgcactgcac agggccatta ctgcgattac
    56521 tgctgcgaat tttttcatgt ttattactcc ctttaatgtc tgtttccgag catttaacaa
    56581 gatagtcctt aacaacttct taagggaaaa aaataaaatt taatgctgta cagagcgcgt
    56641 tacaacacgg tttactggca gcaaatacgg ttatcgcagg gatgaaaaaa tgcgaatttt
    56701 actgatagaa gatgacatgc tgattggtga cggcatcaaa actggcctta gtaaaatggg
    56761 ttttagtgtc gactggttta cacaaggtcg tcagggaaaa gaggcgcttt atagcgcacc
    56821 ttatgatgcg gtgatcctgg atttaacctt accaggcatg gatggtcgcg atattttgcg
    56881 cgaatggcga gagaaaggtc agcgtgagcc ggtactgaac ctgaccgcgc gtgatgcgtt
    56941 agcggaacgt gtagaagggc tgcgtctggg agctgacgat tatctgtgta aaccttttgc
    57001 gttgatagaa gtcgccgcca agctggaggc tctgatgcgc cgaaccaacg gccaggccag
    57061 caacgagctg cgccacggta acgtcatgct cgaccccggc aaacgtatcg ccacgctggc
    57121 tggcgaaccc ttaacactga aaccaaaaga atttgccctg ctggaattac tgatgcgtaa
    57181 cgctggtcgg gtactgccgc gcaaactgat tgaagagaaa ctgtatacct gggacgaaga
    57241 ggtcaccagt aatgccgttg aagtgcatgt gcatcatctg cgacgcaaac tcggtagtga
    57301 ttttattcgt accgtgcatg gtattggcta cacattaggt gagaaatgaa atttacccaa
    57361 cgtcttagtc tgcgcgtcag gctgacgcta atctttttaa ttctggcctc ggtgacctgg
    57421 ctgctttcca gctttgtcgc ctggaaacaa acaacggata acgtcgatga attgttcgac
    57481 acccaactga tgctgtttgc caaacggtta agtacgctcg atctcaacga aatcaacgcg
    57541 gcggatcgca tggcacagac gccaaataaa ttaaaacacg gtcatgttga tgacgatgcg
    57601 ctgacctttg ccatctttac ccacgacggc agaatggtcc ttaatgatgg cgataacgga
    57661 gaagatattc cctatagcta tcaacgggaa ggttttgctg acgggcaact ggtcggtgaa
    57721 gacgatcctt ggcgttttgt ctggatgacc tcacctgatg gcaaatatcg catcgttgtt
    57781 ggccaggaat gggaataccg tgaagacatg gcgctggcga ttgttgccgg gcaattgatc
    57841 ccgtggctgg tcgcactgcc gattatgtta atcatcatga tggtactact gggtcgtgaa
    57901 ctcgcgccgc tgaacaaact ggcgctggca ctacgtatgc gtgaccctga ctcggaaaaa
    57961 ccactaaacg cgactggcgt acccagcgaa gtgcggccac tggttgagtc gctaaatcaa
    58021 ctgttcgccc gcacacatgc gatgatggtt cgagaacgac gctttacctc cgatgcagct
    58081 cacgaactac gtagcccgtt aacagcgctg aaagtgcaaa ccgaagttgc gcagctctct
    58141 gacgatgatc cgcaggcgcg gaaaaaagca ctgctccaat tacattccgg gatcgatcgc
    58201 gctactcgtc tggttgatca actgctcacg ctatcgcggc tggactcact ggataacctt
    58261 caggacgtcg cggagatccc gcttgaagat ctcctacaat cgtcggtgat ggatatttac
    58321 cacacggcgc agcaggcgaa aattgacgtg cgactgacac tcaatgccca cggcatcaaa
    58381 cgcaccggac aaccgctatt gctaagtttg ttggtgcgaa atttgctgga taacggcccc
    58441 ggtgtgacac cagaggcact ggcgcgaatt ggcgaacgct tctatcgccc acccggacaa
    58501 accgctaccg gcagtggact tgggctatcg attgtccagc gaatcgccaa gctgcatgac
    58561 atgaatgttg agtttgggaa tgcggaacaa ggtggatttg aggcgaaggt aagctggtaa
    58621 cttctctgtc tatgagagcc gttaaaacga ctctcataga ttttactaat agcaaaatat
    58681 aaaccgtccc caaaaaagcc accaaccaca aaccagacaa ataacgagcg agaatacata
    58741 tagccaaaac tcgaacattt cccgcataaa gagttttctt aagataagaa taataagtgg
    58801 cgtaagaaga aaaaatgctg caatcattgc ttgcaatata cgatcttcgc ctgaaacgga
    58861 aggtatactg caaatattgc ctcccgactc aagcatccag tcatacttat tgaaagcaaa
    58921 aaaagaatct attaaccaat aagtaagcat tatatatagc aatgcttcaa atattgtttt
    58981 aattttctgc tttaacatat atcagtacgc tcataaaaat ccatttataa ttatcaacat
    59041 gacacgttga tattttcatc ttttacttat tcaaagccat attttcaatc cccaaaaaaa
    59101 ccgaaccaaa cgtgtaaatt attccggtgc gagtaagaat tatgaggaat ggctatcagt
    59161 attgtcattt tcagaaaata tttatcctgc atcggtgagt cagagctaga tcagactttt
    59221 gctaaattcg caaaagactt tgcacatttt gctaatttca cagtaccgct ctgtgacgta
    59281 ctatagtcga caaacgtctc accttgagat taaaaaatga gcaacatcct gattatcaac
    59341 ggcgcgaaaa aattcgccca ctccaatggt caactgaacg acaccctgac cgaagtcgcg
    59401 gatggcacac tgcgcgacct cgggcatgat gtccgcatcg ttcgcgccga cagcgactac
    59461 aatgtcaaag cggaagtaca aaactttctc tgggctgatg tggtgatctg gcagatgcca
    59521 ggctggtgga tgggcgcgcc gtggacagtg aaaaaatata ttgatgatgt attcaccgaa
    59581 ggtcacggga ctctgtatgc cagcgatggt cgtacccgca aagatccgtc gaaaaaatac
    59641 ggttccggca gcctggtaca gggcaaaaaa tatatgcttt ctctaacctg gaacgcacca
    59701 atggaagcct tcaccgaaaa agatcagttc ttccacggcg ttggcgttga cggtgtgtat
    59761 ctgccgttcc ataaagcaaa ccaattcctc ggtatggaac cgctgccgac atttatcgct
    59821 aatgacgtga taaaaatgcc tgatgttccc cgctatactg aagaatatcg caagcatctt
    59881 gtggaaattt ttggttaact agagctcagg ctttagaagg agttaaccat gcttaccgta
    59941 atcgcagaaa tccgtactcg tcctggtcaa catcaccgtc aggcggtatt ggatcagttt
    60001 gctaaaatcg ttccaaccgt actgaaagaa gaaggttgcc acggctatgc gccaatggtg
    60061 gattgcgctg ctggcgtgag tttccagtct atggcaccgg attctatcgt gatgattgag
    60121 cagtgggaaa gcatcgcgca ccttgaagcg catctgcaaa ccccgcacat gaaggcgtac
    60181 agcgaagccg taaaaggcga cgtgctggag atgaatatcc gtattctgca gccagggatt
    60241 taatcctgcc ttgtttgccc ggccatcctg accgggcaat gttctttcct ttaaacctca
    60301 atctccgcca tgtcgccttt ctcttgcaac cagttgcggc gatcttccga gcgtttcttc
    60361 gccagcagca tatccatcat cgcgtcagta cgctgatcgt cttcatcatc gatagtcaac
    60421 tgcaccagac ggcgagtgtt cggatcaagc gtggtttcac gcaattgcat cgggttcatt
    60481 tcccccagac ctttaaaacg ctggacgttc ggcttgcctt tcttgcgttt taattgctca
    60541 agtacgccct ctttctcttc ttccgtcagc gcgtaataaa cctctttccc gagatcaata
    60601 cggtagagcg gtggcagtgc gacgtaaacg tgaccgtgtt tcaccaacgc gcggaagtgt
    60661 tttacgaaca aagcgcagag cagcgtggca atgtgcagac catcagagtc cgcatccgcg
    60721 aggatacaga ttttgccata acgaagctgg ctcagatcgt cgctgtcagg atcgataccg
    60781 atcgctaccg aaatatcatg cacttcctgc gaggccagca cttcgtcgga agaaacttcc
    60841 caggtgttaa ggatcttacc tttcagcggc atgatcgcct gatattcacg atcgcgcgcc
    60901 tgcttggcag atccgcctgc ggagtcacct tccactagga acagttcggt acggttaagg
    60961 ccctgcgcgg tacaatcagc cagtttgcca ggcagcgccg ggccgctggt cagcttttta
    61021 cgcaccactt ttttagccgc acgcatacga cgctgggcgc tggaaatcgc catctctgcc
    61081 agcaactccg ccgcctgaac gttctggttc agccacagga tgaaggcatc tttcaccacg
    61141 cccgaaacga atgccgcaca ctgacgcgaa gagagacgct ctttggtctg cccggcaaac
    61201 tgcggatcct gcattttaac cgacagaaca taggcgcagc gatcccagat atcttccgcc
    61261 gacagcttca cgccgcgcgg cagaatgttg cggtattcac agaactcacg catcgcgtcc
    61321 aacaggccct gacgcagacc attaacatgg gtaccgccct gcatcgttgg gataaggttg
    61381 acgtagcttt cggtcagcag ttcaccgcct tccggcagcc acagtagcgc ccagtccaca
    61441 gcttcagtat cgccagcgaa attaccgata aacggttttt ctggcagcgt cggtaaaccg
    61501 tttaccgctt ccgccaggta atcattcaga ccgtcctgat agcaccagcg ctgttcggtg
    61561 ttgttgatct catctttaaa ggtaatctca acgccggggc acagtactgc tttggccttc
    61621 agcacatgcg tcaggcgtga aacagaaaaa cgcgggctgt caaagaaggt ttcatccggc
    61681 cagaagtgca cgctggtacc ggtattgcgt ttaccgcaag tgccgacaac ctgtaaatcc
    61741 tgcacctttt cgccattttc aaaggcgatg ttataaacct gaccatcgcg gcgcacgtta
    61801 acttctacgc gcttcgacaa ggcgttaact accgaaatcc ccacgccatg caggccgcca
    61861 gagaactggt aatttttgtt agagaattta ccgcctgcat gcagacggca aagaatcagt
    61921 tcaaccgccg gtactccttc ttccgggtga atatccaccg gcatcccgcg cccatcgtca
    61981 atcacttcta acgactggtc agcatgtaag attacatcca cgcgttttgc gtgacccgcc
    62041 agtgcttcat cgacactgtt atcaataact tcttgcccca aatggttagg gcgagtggta
    62101 tcggtataca tccccggacg gcggcgaacc ggctcaagcc cggtgagtac ctcaatggca
    62161 tcagcgttat aagtttgcgt catggtttaa gttagcaatt cgagttgatc gtcagagatg
    62221 gtgcagacca agaaaatcga cgatcgggtt gaaataatct tcgaagcccg tgaatgcgtg
    62281 gttgccgcct tctatgacag tctggcggca ggaagcgtag tacgccaccg cctggcggta
    62341 atccagcact tcatctcccg tctgttgcag cagccagatc aaatccggcg cttccagcgg
    62401 gtcaatctgc atgactttga gatcgtaaat atggcgtgac tctagcacat attgctgccc
    62461 ggtgtagggg ttctcgttct gaccgagata gtccgtcagc aattcaaacg ggcgtaccgc
    62521 cgggtttacc accactgcgg gcagcataaa acattgcgac aaccaggtgg cgtaatatcc
    62581 ccccagtgac gaaccgacaa tacccagcga atcgccgcca tgttccagga caatggattc
    62641 cagcagttct gccgcgtcgg aaggatacgg cggcaactgc ggaatgatca tctcaacgtc
    62701 agggtgatgt tccgccagcc agttttttaa caagctcgct tttgcagagc gcggcgagct
    62761 gttgaaaccg tgtaaataaa gaagcgtaga catcagtagc cttctgaagc ggtatcaggt
    62821 tggaaacgtg tgtccgccag gcgatgcacc tcggtggtca gcgtgccatc agcatgtaac
    62881 tcgagagtac gccagccggg cgcgatagta tccagcgtaa agttggaaca gtgcggctta
    62941 aactgcacac aggtcgacgg cgttgccagc aggcggcgac cgttccagtc gagatccagc
    63001 tcctgatgaa tatgaccgca cagcaagtat ttgacgtgcg gaaacttcgc cagcacggta
    63061 tccagttcgc ccgcgttacg cagactgtgt tgatcgagcc aactacaacc cgcaggtagc
    63121 ggatgatgat gcagcagcag caacgtatgg cgttctggcg catcggccag tttacgttcc
    63181 agccactcaa gctgaaactc gctcagctca ccgtgcggca cgccaaacac ctggctatcc
    63241 agcaacagga tttgccattg ctcaccaata aacacccgct tcgccgggga gatacccgca
    63301 tcctgtaacg cgctgtacat cgcgggctgg aaatcgtggt tgcccggcag ccagacgcag
    63361 ggcgcacgaa aacttgcgat gccttcagcg aaatgctgat aggccgcaga ggattgatcc
    63421 tgcgctaaat cacctgtcgc gacaatcagg tcgaattcgt gctggtgtgg acgaatcgcc
    63481 tccagcaccg cctggtagct ttcccaggtg tttaccccta acagggcttc gtgcttttgt
    63541 gcaaacaggt gagtatcggt aatttgtaaa atccttcctc tggcctcacc agccagagga
    63601 agggttaaca ggctttccaa atggtgtcct taggtttcac gacgctagta aaccggaatc
    63661 gccatcgctc catgtgctaa acagtatcgc aaccagtccg ctaaaaactg gttaatttga
    63721 tgcttttcgt cgcgttgatg caacttttta ttaggataat cataccgcgc tttgaagcga
    63781 aaaatctgct ggcttgaaca cacttcagcc accatcgcgt catgatacag acgcaccgtc
    63841 attgacggaa ggctccagta actgatcgcg ggcgcagtct gttctattgt cactagggta
    63901 gtgtatcggg tcgattccac aatcgtcagc cgatattgtg cgtttgccac ctgatagctt
    63961 acagtttcgc cgggtgcgtc attgcgcggt aacaaacggc gcaattgtga aaagttcatc
    64021 tcgcacaggc gcatcatttc aggaaagtca ggtgtgtaac gcttcattta tgcccactca
    64081 ttttttaacg cttgatgatg cagctgcagc cattgcaaag cgatgaccga cgctgcgttg
    64141 tcgattttcc cctcttctac ccactggtat gcctgttccc ggcttaccac atgaacgcga
    64201 atatcttcgt tttcatcagc cagaccatga ataccgcttg cggtcgtggc gtccacttcg
    64261 cccaccataa ttgacgaacg ctcactggtg ccccccgggc ttgccaggaa acttaacacc
    64321 ggtttggtcc gtttgactat cagtcccgcc tcttcaatcg cttcgcgacg ggcaacatct
    64381 tccacacttt caccctcttc aatcatcccg gcaaccatct ccagtagcca gggggtttcg
    64441 ctggtgtcgt acgcggcaat ccgaatctgc tcaatcagca caacttcatc acgcactggg
    64501 tcaaagggta gcaagactgc ggcgtgaccg cgctcaaaaa tttcccgccg tacctcatga
    64561 ctcatttgcc cgttgaatag acgatgacga aatctataaa gatctaatga aaaaaagccg
    64621 cgataaagtg tttctcgtgc aataatttct acatcgtttt tgccaaatgt aacgggcagg
    64681 ttgtctggct taagcattgt taatgtccta gcactaatag tgaattaaat gtgaatttca
    64741 gcgacgtttg actgccgttt gagcagtcat atgttaaatt gaggcacatt aacgccctat
    64801 ggcacgtaac gccaaccttt tgcggtagcg gcttctgcta gaatccgcaa taattttaca
    64861 gtttgatcgc gctaaatact gcttcaccac aaggaatgca aatgaagaaa ttgctcccca
    64921 ttcttatcgg cctgagcctt tctgggttca gttcgttgag ccaggccgag aacctgatgc
    64981 aagtttatca gcaagcacgc cttagtaacc cggaattgcg taagtctgcc gccgatcgtg
    65041 atgctgcctt tgaaaaaatt aatgaagcgc gcagtccatt actgccacag ctaggtttag
    65101 gtgcagatta cacctatagc aacggctacc gcgacgcgaa cggcatcaac tcgaacgcga
    65161 ccagtgcgtc cctgcagtta actcaatcca tttttgatat gtcgaaatgg cgtgcgttaa
    65221 cgctgcagga aaaagcagca gggattcagg acgtcacata tcagaccgat cagcaaacct
    65281 tgatcctcaa caccgcgacc gcttatttca acgtgttgaa tgctattgac gttctttcct
    65341 atacacaggc acaaaaagaa gcgatctacc gtcaattaga tcaaaccacc caacgtttta
    65401 acgtgggcct ggtagcgatc accgacgtgc agaacgcccg cgcgcagtac gataccgtgc
    65461 tggcgaacga agtgaccgca cgtaataacc ttgataacgc ggtagagcag ctgcgccaga
    65521 tcaccggtaa ctactatccg gaactggcgg cgctgaatgt cgaaaacttt aaaaccgaca
    65581 aaccacagcc ggttaacgcg ctgctgaaag aagccgaaaa acgcaacctg tcgctgttac
    65641 aggcacgctt gagccaggac ctggagcgcg agcaaattcg ccaggcgcag gatggtcact
    65701 taccgactct ggatttaacg gcttctaccg ggatttctga cacctcttat agcggttcga
    65761 aaacccgtgg tgccgctggt acccagtatg acgatagcaa tatgggccag aacaaagttg
    65821 gcctgagctt ctcgctgccg atttatcagg gcggaatggt taactcgcag gtgaaacagg
    65881 cacagtacaa ctttgttggt gccagtgagc aactggaaag cgcacatcgt agcgtcgtgc
    65941 aaaccgtacg ttcctccttc aacaacatta atgcttctat cagtagtatt aacgcctaca
    66001 aacaagccgt agtttccgcg caaagctcat tagacgcgat ggaagcgggc tactcggtcg
    66061 gtacgcgtac cattgttgat gtgttggatg cgaccaccac gctgtacaac gccaagcaag
    66121 agctggcgaa tgcgcgttat aactacctga ttaatcagct gaatattaag tcagccctgg
    66181 gtacgttgaa cgagcaggat ttgctggcac tgaacaatgc gctgagcaaa ccggtttcca
    66241 ctaatccgga aaacgttgcc ccgcaaacgc cggaacagaa tgctattgct gatggttatg
    66301 cgcctgatag cccggcaccc gtcgttcagc aaacatccgc acgcactacc accagtaacg
    66361 gtcataaccc tttccgtaac tgatgacgac gacggggctt cggccccgtc tgaacgtaag
    66421 gcaacgtaaa gatacgggtt atctgccgca ttcttccccc ttctcgcttc aatttcgacc
    66481 agccatcctc tattctgatg ggtatttacc actggtcccg gaagacaaaa atgaaacgga
    66541 caaaatccat acgccacgca tcgttccgca aaaactggag cgcacgccat ctgacaccag
    66601 tcgctctcgc ggttgccact gtttttatgc tggctggctg tgaaaagagt gatgaaacag
    66661 tgtctctcta tcaaaatgct gacgactgtt cagctgcaaa cccaggcaaa agcgccgaat
    66721 gtaccaccgc gtacaacaat gcgctgaaag aagccgaacg tactgcgccg aaatacgcca
    66781 cccgtgaaga ctgtgttgct gaatttggtg aaggtcagtg tcagcaagca ccagcccagg
    66841 ctggcatggc accagaaaac caggcgcagg ctcagcaatc cagcgggagt ttctggatgc
    66901 cgctgatggc cggttacatg atggggcgtc tgatgggcgg cggcgcggga tttgcacagc
    66961 agccgctgtt ctcctcgaaa aacccggcca gtccggctta cggtaaatat accgacgcga
    67021 cgggtaaaaa ctatggcgca gcccagccag gccgcaccat gaccgtaccg aagacggcaa
    67081 tggcaccaaa accggcgacc accactaccg ttacccgtgg cggttttggt gaatctgttg
    67141 ccaaacaaag cactatgcag cgtagcgcaa ccggcacctc ttctcgttca atgggtggct
    67201 gataccgatg gaaagagtca gtattaccga gcgcccggac tggcgtgaga aagcccacga
    67261 atacggtttc aattttcaca ccatgtacgg cgagccgtac tggtgtgaag atgcttacta
    67321 caagttgacc ctcgcccagg ttgaaaagct ggaagaagtc accgccgaac tgcaccagat
    67381 gtgcctgaaa gtggtggaaa aagtgatcgc cagcgatgag ctgatgacca aattccgcat
    67441 accaaaacac acctggagtt ttgtgcgtca atcatggctg actcaccagc catcgcttta
    67501 ttcgcgtctt gatctggcgt gggatggcac tggcgaacct aaacttctgg aaaataacgc
    67561 cgatacgcca acgtcactat acgaggcggc gttctttcag tggatctggc tggaagatca
    67621 gcttaacgcc ggtaacttgt cggagggcag cgaccagttt aacagtctgc aagaaaaact
    67681 gatcgatcgc ttcgttgagc tgcgtgaaca gtatggcttc cagttgctgc atctcacctg
    67741 ctgtcgcgac acggtggaag atcgcggaac cattcagtat ttgcaggact gcgcaaccga
    67801 agctgaaatc gccaccgagt tcctctacat cgacgagata ggtttaggcg aaaaaggcca
    67861 gttcacggat ttacaggatc aggtgatttc taacctgttc aagctttatc cgtgggaatt
    67921 tatgctgcgc gaaatgttct ccaccaagct ggaagatgct ggcgtacgct ggctggaacc
    67981 ggcgtggaag agcattatct ccaacaaggc acttctaccg ctactgtggg agatgttccc
    68041 gaatcacccg aacctgctgc ccgcttattt tgcggaagat gatcatccgc aaatggaaaa
    68101 atatgtggtt aaaccgatct tctcccgtga aggcgcaaac gtgtcgatca ttgagaacgg
    68161 caaaaccatt gaagcagcgg aaggtccgta tggcgaagaa gggatgattg ttcagcaatt
    68221 ccacccgtta ccgaaattcg gcgacagcta tatgctgatt ggtagctggc tggtgaacga
    68281 tcaacctgcc ggaattggca ttcgtgaaga ccgtgcgttg atcacccagg atatgtctcg
    68341 gttttatcca catatttttg ttgaataagc cacgataccg gatggcactc gccatccggt
    68401 aattgttagc ctatctgcac cgacagcata cttaaactgc ccatttctat accatcgacc
    68461 ggaatggtaa ttggctcctg cccatcccac acacctaaca catacaacaa cggcagatag
    68521 tgctctggcg ttgggttcga taacgcgcca ccttcatggt cgagataatt caccagagga
    68581 tgctgctcta ccggcccttg ccacgtcaga ttcgctttca catactcatt aaacgacgtc
    68641 gcccacggat acggtgaact atcaccgtgc cacttcactg tgcgcaggtt atgcaccacg
    68701 ttaccgctgg cgaccaacat tattccttca tcacgcagcg ccgcgagttt gcgccccatt
    68761 tcgaaatgcc aggcgacagg tttgctactg tcgatactca actgcaccat agggatatcg
    68821 gcgtcaggat acatcttaat cagcacgccc cacgagccgt ggtcaaagcc ccaggcttct
    68881 ttatccagcg tcaccgggat cggcgctaac agctcaacca gacgctgtgc cagcgcaggc
    68941 gaacccggag caggataatg cgtatcgtac agcgcctgcg ggaagccacc aaagtcatga
    69001 atcgtgggcg gcgtctccat cgcggtcact cctgttccac gggtaaacca gtgagccgaa
    69061 accaccacaa tcgcttgcgg gcgtggcaat gtcatcccca acttctgcca gctgcgggta
    69121 tacaaattat cttccagcac gttcatcgga ctaccgtgac ctaaaaacaa tgctggcata
    69181 cgtgttgaag acatgatgat atccttaact aaaggtgtca ttttgatatc ctcacaatac
    69241 gcttgttcgg cggagtaaga acccggataa caatgatgat gatcatcagt tattttgacg
    69301 atctgcctga aggtgaagat ttataaggag ttgtcgatgt cagtacctct cattctgacc
    69361 atactggcgg gggcagccac gtttattggc gcgtttctcg gcgttctcgg acaaaaaccc
    69421 tcgaaccgct tactggcgtt ttcgctaggt tttgcggcgg ggatcatgtt gctcatctca
    69481 ttaatggaaa tgcttcctgc cgcactagca gctgaaggaa tgtcgccggt gttgggttat
    69541 ggaatgttta tcttcggtct gcttggctac tttggcctgg accgcatgtt gccgcatgcg
    69601 catccgcagg atttaatgca aaaatcggtg cagctgttgc caaaatcgat caagcgcaca
    69661 gccattctgc tcactctcgg catcagtctg cataacttcc cggaagggat tgccaccttt
    69721 gtcacggcga gcagcaacct ggagctggga tttggcatcg cactggccgt cgcgttgcac
    69781 aatatccctg aaggtctggc agtggcaggc ccggtttatg cggcaacggg gtctaaacgc
    69841 accgcgattc tgtgggcggg gatttctgga ctagcagaaa ttcttggtgg tgtgctggcg
    69901 tggttaatcc tcggtagcat gatttccccg gtggtcatgg cggcaatcat ggcggcggtt
    69961 gcaggaatta tggtggcgct ctcggttgat gaattaatgc cgctcgccaa agagattgac
    70021 cctaataata accccagcta tggcgtacta tgtggaatgt cagtgatggg attcagttta
    70081 gtgctgctac aaacggcggg aattggttaa aaacaacatc agaagttgtc cagcacatgg
    70141 acaacttctg ccagcttcac gaaagcctta ttctaccttt tcggctgtct cttctctcgt
    70201 actgtttaag tatttgttcc gcgcttcaag cagtgcgtct ctgtcctggc gaaacgcccg
    70261 acaataacgt ttcaaatgca ggaaataacc caccacaacc agagcaatca atacaatcca
    70321 gtaccaggcg ataaacatcc ttttaccctt aatatttaac aatactaaac atttagcgca
    70381 taaatttcac atatcctttt tcggatatat tcatatggtc gcataataac ggaacaatta
    70441 tatgattttg atcataatga aaaagaatgc ctgatattaa tgaaattttc tcatagaaag
    70501 agaagcggaa ttaacatctt gcatagcagg gcaaaataaa aaccgaagcc agctacaact
    70561 tcggtttctt gattaaggca gtaattaagc agcggttttc agctggcttt acgctcatgt
    70621 gcctgacggt atgccaccag gtcttcaata gtcacgagcg ccatattgtg tttattggca
    70681 aactcaatgc actctggtgc acgcgccatc gtgccatcgt cattagtcag ctcacacagc
    70741 acaccagccg gtttaaagcc tgccagcgtc atcaaatcaa tagtcgcttc agtatgaccg
    70801 ccacgcgtca gtacaccacc tgcctgcgcg cgaagtggga aaacgtggcc cgggcgattc
    70861 agatcagaag gttttgcgcc atcggcaatc gctgcgcgaa cggtcgtaat acgatcagcg
    70921 gcagaaacac cggtagtcac accttcagcc gcttcaatgg tcacggtaaa accggtgcca
    70981 taggcgctgg tgttattttc taccatcatt ggcagatcaa gttgtttacg gcgatcttca
    71041 gtgatgcaca ggcaaacaat accgctaccg tggcgaatgg tcagcgccat ctgctcaacg
    71101 gtcatggttt ctgccgggaa gatcatatcg ccttcgtttt cacggtcttc atcatcaagc
    71161 accattacac cgcgtccttc acgcagcgca gccagtgcat tttcaacacg ttcgaaaggc
    71221 gtaccaaaag aggaaagtag cgtctgattc atggtaaaaa aacctcacta aaattatggt
    71281 taccagaatc agggcagtct taggagtggc ggcatatagc caaaataacg tgagcgggtc
    71341 catgcccgac agaatcgtta ctctctccca tccggactct aaccgtcggc cccggaatta
    71401 caccggatct gctgtccttt gagttcgcac ccaaagcgct cgcgggcttt caactgagtt
    71461 gatttaccgc cggtggggaa tttcgccccg ccctgagaat aagcggattc actataacgc
    71521 taatgattag cggcagcaac gcatagcttc acataattct ggtttatgac ttacccttat
    71581 cgcactacaa tggtactcaa cacctatcag tacagggaaa ctacaatgat tgacccgaaa
    71641 aaaattgagc aaatcgctcg ccaggttcac gaatcaatgc ctaaaggagt cagggagttc
    71701 ggggaagatg tggagaaaaa aatccgcc
//
LOCUS       DAJTQY010000007        70705 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.007, whole genome shotgun sequence.
ACCESSION   DAJTQY010000007 DAJTQY010000000
VERSION     DAJTQY010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 70705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70705
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.007"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            <1..795
                     /locus_tag="OO349_000464"
     CDS             <1..795
                     /locus_tag="OO349_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5865661.1"
                     /translation="WLPRSLVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLD
                     DDNNHNSSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAF
                     ALIDANGASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQ
                     FVVPNRGAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDDTGQQSIVDEGGILY
                     LSGISSKSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            806..1897
                     /locus_tag="OO349_000465"
     CDS             806..1897
                     /locus_tag="OO349_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5865662.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKLND
                     SLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKSSTVYSAATQTGVCQGDTSRYGPVNI
                     GANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLTA
                     PQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQMR
                     IDGTTGVVDQYNLVARRRSSDNVPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTVN
                     MRAWPVNLVGGELETGKFQGTAAITVMVR"
     gene            complement(1940..2800)
                     /gene="rsmI"
                     /locus_tag="OO349_000466"
     CDS             complement(1940..2800)
                     /gene="rsmI"
                     /locus_tag="OO349_000466"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HCR5865663.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRTLEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            2865..4901
                     /gene="lpoA"
                     /locus_tag="OO349_000467"
     CDS             2865..4901
                     /gene="lpoA"
                     /locus_tag="OO349_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HCR5865664.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAAEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTADSVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSALGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            4859..5254
                     /locus_tag="OO349_000468"
     CDS             4859..5254
                     /locus_tag="OO349_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HCR5865665.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGLTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            5274..5864
                     /gene="diaA"
                     /locus_tag="OO349_000469"
     CDS             5274..5864
                     /gene="diaA"
                     /locus_tag="OO349_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HCR5865666.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            5874..6449
                     /gene="yraP"
                     /locus_tag="OO349_000470"
     CDS             5874..6449
                     /gene="yraP"
                     /locus_tag="OO349_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HCR5865667.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(6563..7603)
                     /locus_tag="OO349_000471"
     CDS             complement(6563..7603)
                     /locus_tag="OO349_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR5865668.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMALLIATLVQKWVRETLQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVCILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(7676..8311)
                     /locus_tag="OO349_000472"
     CDS             complement(7676..8311)
                     /locus_tag="OO349_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HCR5865669.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            8439..8957
                     /gene="yhbO"
                     /locus_tag="OO349_000473"
     CDS             8439..8957
                     /gene="yhbO"
                     /locus_tag="OO349_000473"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HCR5865670.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGVEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(8937..9380)
                     /locus_tag="OO349_000474"
     CDS             complement(8937..9380)
                     /locus_tag="OO349_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HCR5865671.1"
                     /translation="METLIAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            9431..9733
                     /gene="yhbQ"
                     /locus_tag="OO349_000475"
     CDS             9431..9733
                     /gene="yhbQ"
                     /locus_tag="OO349_000475"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HCR5865672.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(9720..10223)
                     /locus_tag="OO349_000476"
     CDS             complement(9720..10223)
                     /locus_tag="OO349_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR5865673.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFLVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(10217..10741)
                     /locus_tag="OO349_000477"
     CDS             complement(10217..10741)
                     /locus_tag="OO349_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HCR5865674.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNTQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGVYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            10950..11945
                     /locus_tag="OO349_000478"
     CDS             10950..11945
                     /locus_tag="OO349_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCR5865675.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     HYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            11954..12832
                     /locus_tag="OO349_000479"
     CDS             11954..12832
                     /locus_tag="OO349_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCR5865676.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAAASSADVIYLGEAVCSKRRA
                     TKVGDCLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAVPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            12913..13920
                     /locus_tag="OO349_000480"
     CDS             12913..13920
                     /locus_tag="OO349_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCR5865677.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHLPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYDV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(14038..15282)
                     /gene="mtr"
                     /locus_tag="OO349_000481"
     CDS             complement(14038..15282)
                     /gene="mtr"
                     /locus_tag="OO349_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HCR5865678.1"
                     /translation="MATRTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIALIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(15436..17325)
                     /gene="deaD"
                     /locus_tag="OO349_000482"
     CDS             complement(15436..17325)
                     /gene="deaD"
                     /locus_tag="OO349_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HCR5865679.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(17318..17398)
                     /gene="yrbN"
                     /locus_tag="OO349_000483"
     CDS             complement(17318..17398)
                     /gene="yrbN"
                     /locus_tag="OO349_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HCR5865680.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(17505..18389)
                     /gene="nlpI"
                     /locus_tag="OO349_000484"
     CDS             complement(17505..18389)
                     /gene="nlpI"
                     /locus_tag="OO349_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HCR5865681.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(18498..20633)
                     /gene="pnp"
                     /locus_tag="OO349_000485"
     CDS             complement(18498..20633)
                     /gene="pnp"
                     /locus_tag="OO349_000485"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HCR5865682.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSREGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(20880..21149)
                     /gene="rpsO"
                     /locus_tag="OO349_000486"
     CDS             complement(20880..21149)
                     /gene="rpsO"
                     /locus_tag="OO349_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HCR5865683.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(21298..22242)
                     /gene="truB"
                     /locus_tag="OO349_000487"
     CDS             complement(21298..22242)
                     /gene="truB"
                     /locus_tag="OO349_000487"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HCR5865684.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERIVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(22242..22643)
                     /gene="rbfA"
                     /locus_tag="OO349_000488"
     CDS             complement(22242..22643)
                     /gene="rbfA"
                     /locus_tag="OO349_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HCR5865685.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(22807..25455)
                     /gene="infB"
                     /locus_tag="OO349_000489"
     CDS             complement(22807..25455)
                     /gene="infB"
                     /locus_tag="OO349_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HCR5865686.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSNQQDDMTKN
                     AQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDNAEPTEDSS
                     DYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADREEARAAVRG
                     GKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIKAMMKLSAM
                     ATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAPVVTIMGHV
                     DHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAAFTSMRARG
                     AQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPDRVKNELSQ
                     YGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMASGAVIESFL
                     DKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSIPVEILGLS
                     GVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMTEGEVHEVN
                     IVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASNAILVGFNV
                     RADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLAEVRDVFKS
                     PKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVRNGMECGIG
                     VKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(25480..26967)
                     /gene="nusA"
                     /locus_tag="OO349_000490"
     CDS             complement(25480..26967)
                     /gene="nusA"
                     /locus_tag="OO349_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HCR5865687.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(26995..27447)
                     /gene="rimP"
                     /locus_tag="OO349_000491"
     CDS             complement(26995..27447)
                     /gene="rimP"
                     /locus_tag="OO349_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HCR5865688.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(27654..27730)
                     /locus_tag="OO349_000492"
     tRNA            complement(27654..27730)
                     /locus_tag="OO349_000492"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27694..27696),aa:Met,seq:cat)
     gene            28078..29421
                     /gene="argG"
                     /locus_tag="OO349_000493"
     CDS             28078..29421
                     /gene="argG"
                     /locus_tag="OO349_000493"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HCR5865689.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGLPQVENMENKGQ"
     gene            complement(29429..31036)
                     /locus_tag="OO349_000494"
                     /pseudo
     CDS             complement(29429..31036)
                     /locus_tag="OO349_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011378876.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
     gene            complement(31514..31600)
                     /locus_tag="OO349_000495"
     tRNA            complement(31514..31600)
                     /locus_tag="OO349_000495"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31564..31566),aa:Leu,seq:gag)
     gene            complement(31615..31947)
                     /gene="secG"
                     /locus_tag="OO349_000496"
     CDS             complement(31615..31947)
                     /gene="secG"
                     /locus_tag="OO349_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HCR5865690.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNRGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(32175..33512)
                     /gene="glmM"
                     /locus_tag="OO349_000497"
     CDS             complement(32175..33512)
                     /gene="glmM"
                     /locus_tag="OO349_000497"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HCR5865691.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(33505..34353)
                     /gene="folP"
                     /locus_tag="OO349_000498"
     CDS             complement(33505..34353)
                     /gene="folP"
                     /locus_tag="OO349_000498"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HCR5865692.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(34443..36377)
                     /gene="ftsH"
                     /locus_tag="OO349_000499"
     CDS             complement(34443..36377)
                     /gene="ftsH"
                     /locus_tag="OO349_000499"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HCR5865693.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(36477..37106)
                     /gene="rlmE"
                     /locus_tag="OO349_000500"
     CDS             complement(36477..37106)
                     /gene="rlmE"
                     /locus_tag="OO349_000500"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HCR5865694.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            37232..37525
                     /gene="yhbY"
                     /locus_tag="OO349_000501"
     CDS             37232..37525
                     /gene="yhbY"
                     /locus_tag="OO349_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HCR5865695.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(37681..38157)
                     /gene="greA"
                     /locus_tag="OO349_000502"
     CDS             complement(37681..38157)
                     /gene="greA"
                     /locus_tag="OO349_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HCR5865696.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            38405..39838
                     /gene="dacB"
                     /locus_tag="OO349_000503"
     CDS             38405..39838
                     /gene="dacB"
                     /locus_tag="OO349_000503"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HCR5865697.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPSDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     KPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(40024..41196)
                     /gene="cgtA"
                     /locus_tag="OO349_000504"
     CDS             complement(40024..41196)
                     /gene="cgtA"
                     /locus_tag="OO349_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HCR5865698.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKVIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(41212..42177)
                     /locus_tag="OO349_000505"
     CDS             complement(41212..42177)
                     /locus_tag="OO349_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR5865699.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(42304..42561)
                     /gene="rpmA"
                     /locus_tag="OO349_000506"
     CDS             complement(42304..42561)
                     /gene="rpmA"
                     /locus_tag="OO349_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HCR5865700.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(42582..42893)
                     /gene="rplU"
                     /locus_tag="OO349_000507"
     CDS             complement(42582..42893)
                     /gene="rplU"
                     /locus_tag="OO349_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HCR5865701.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            43151..44122
                     /gene="ispB"
                     /locus_tag="OO349_000508"
     CDS             43151..44122
                     /gene="ispB"
                     /locus_tag="OO349_000508"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HCR5865702.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            44349..44627
                     /gene="sfsB"
                     /locus_tag="OO349_000509"
     CDS             44349..44627
                     /gene="sfsB"
                     /locus_tag="OO349_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HCR5865703.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(44675..45934)
                     /gene="murA"
                     /locus_tag="OO349_000510"
     CDS             complement(44675..45934)
                     /gene="murA"
                     /locus_tag="OO349_000510"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCR5865704.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(45989..46243)
                     /gene="ibaG"
                     /locus_tag="OO349_000511"
     CDS             complement(45989..46243)
                     /gene="ibaG"
                     /locus_tag="OO349_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HCR5865705.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(46403..46696)
                     /gene="mlaB"
                     /locus_tag="OO349_000512"
     CDS             complement(46403..46696)
                     /gene="mlaB"
                     /locus_tag="OO349_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HCR5865706.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(46696..47331)
                     /gene="mlaC"
                     /locus_tag="OO349_000513"
     CDS             complement(46696..47331)
                     /gene="mlaC"
                     /locus_tag="OO349_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HCR5865707.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(47350..47901)
                     /gene="mlaD"
                     /locus_tag="OO349_000514"
     CDS             complement(47350..47901)
                     /gene="mlaD"
                     /locus_tag="OO349_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HCR5865708.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTESTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(47906..48688)
                     /gene="mlaE"
                     /locus_tag="OO349_000515"
     CDS             complement(47906..48688)
                     /gene="mlaE"
                     /locus_tag="OO349_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HCR5865709.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(48696..49505)
                     /gene="mlaF"
                     /locus_tag="OO349_000516"
     CDS             complement(48696..49505)
                     /gene="mlaF"
                     /locus_tag="OO349_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HCR5865710.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQTNPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            49715..50680
                     /locus_tag="OO349_000517"
     CDS             49715..50680
                     /locus_tag="OO349_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HCR5865711.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQGTDSLTREQLA
                     ELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMALTAIAIGTSL
                     PELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDYSVML
                     LVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            50694..51680
                     /gene="kdsD"
                     /locus_tag="OO349_000518"
     CDS             50694..51680
                     /gene="kdsD"
                     /locus_tag="OO349_000518"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HCR5865712.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPSEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            51701..52267
                     /gene="kdsC"
                     /locus_tag="OO349_000519"
     CDS             51701..52267
                     /gene="kdsC"
                     /locus_tag="OO349_000519"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HCR5865713.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            52264..52839
                     /gene="lptC"
                     /locus_tag="OO349_000520"
     CDS             52264..52839
                     /gene="lptC"
                     /locus_tag="OO349_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HCR5865714.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            52808..53365
                     /gene="lptA"
                     /locus_tag="OO349_000521"
     CDS             52808..53365
                     /gene="lptA"
                     /locus_tag="OO349_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HCR5865715.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            53372..54097
                     /gene="lptB"
                     /locus_tag="OO349_000522"
     CDS             53372..54097
                     /gene="lptB"
                     /locus_tag="OO349_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HCR5865716.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLISH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            54145..55578
                     /gene="rpoN"
                     /locus_tag="OO349_000523"
     CDS             54145..55578
                     /gene="rpoN"
                     /locus_tag="OO349_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HCR5865717.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            55601..55888
                     /gene="hpf"
                     /locus_tag="OO349_000524"
     CDS             55601..55888
                     /gene="hpf"
                     /locus_tag="OO349_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HCR5865718.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            56006..56497
                     /gene="ptsN"
                     /locus_tag="OO349_000525"
     CDS             56006..56497
                     /gene="ptsN"
                     /locus_tag="OO349_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HCR5865719.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            56543..57397
                     /gene="rapZ"
                     /locus_tag="OO349_000526"
     CDS             56543..57397
                     /gene="rapZ"
                     /locus_tag="OO349_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HCR5865720.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            57394..57666
                     /gene="npr"
                     /locus_tag="OO349_000527"
     CDS             57394..57666
                     /gene="npr"
                     /locus_tag="OO349_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HCR5865721.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            57879..58505
                     /gene="yrbL"
                     /locus_tag="OO349_000528"
     CDS             57879..58505
                     /gene="yrbL"
                     /locus_tag="OO349_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HCR5865722.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGGDKEIRRE
                     LKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCRYE
                     EDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLIPL
                     ATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(58502..59229)
                     /gene="mtgA"
                     /locus_tag="OO349_000529"
                     /pseudo
     CDS             complement(58502..59229)
                     /gene="mtgA"
                     /locus_tag="OO349_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
     gene            complement(59226..59879)
                     /gene="elbB"
                     /locus_tag="OO349_000530"
     CDS             complement(59226..59879)
                     /gene="elbB"
                     /locus_tag="OO349_000530"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HCR5865723.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGVGAAKNLS
                     NFASLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(60109..62445)
                     /gene="arcB"
                     /locus_tag="OO349_000531"
     CDS             complement(60109..62445)
                     /gene="arcB"
                     /locus_tag="OO349_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HCR5865724.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLELNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(62541..63470)
                     /locus_tag="OO349_000532"
     CDS             complement(62541..63470)
                     /locus_tag="OO349_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709009.2"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HCR5865725.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETSVDGIKLHPLHI
                     VKGSIMAKAWEAVRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            64145..68605
                     /gene="gltB"
                     /locus_tag="OO349_000533"
     CDS             64145..68605
                     /gene="gltB"
                     /locus_tag="OO349_000533"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HCR5865726.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHAGKSLYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            68618..70036
                     /gene="gltD"
                     /locus_tag="OO349_000534"
     CDS             68618..70036
                     /gene="gltD"
                     /locus_tag="OO349_000534"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HCR5865727.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGNDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            70220..70699
                     /locus_tag="OO349_000535"
     CDS             70220..70699
                     /locus_tag="OO349_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005085993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HCR5865728.1"
                     /translation="MESLSERTSTGYQQIHNGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIDRLSKDLSRRYKRGVSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKLRGCLIFPQLLYCISYFRFVYSCK"
BASE COUNT        17318 a        19176 c        17547 g        16664 t
ORIGIN      
        1 tggttaccgc gtagcctggt ttcctatcag atgaccagcc agaaagatcg cccaacccaa
       61 catgaaatgc gtcttgatgg ctcactgctg gatgatggtc gcctgagcta tagcctggaa
      121 caaagtctgg atgacgataa caaccataac agtagcctga acgccagtta ccgttcacct
      181 tatggaacct tcagtgccgg atacagttac ggtaatgaca gcagccaata caattacggc
      241 gttaccggcg gcgtggttat ccatcctcat ggcgtgacgc tctcgcaata tctgggcaac
      301 gcttttgcgc ttatcgatgc taacggggct tctggcgtga ggatacaaaa ctatccgggg
      361 attgctactg atccctttgg ctatgcagtg gttccttatc tcacgactta tcaggaaaac
      421 cgtctctcgg tagatactac gcagctgccc gataacgtcg atcttgaaca aacaacacag
      481 tttgtggtgc ccaacagagg tgcaatggta gcggcgcgtt tcaacgccaa tatcggttat
      541 cgcgtacttg ttacagtcag cgatcgcaac ggtaaaccgt tgccctttgg cgctcttgcc
      601 agcaacgatg atacggggca acaaagtatc gtcgatgagg gcggcatact atatctctct
      661 gggatatcga gtaaatcaca aagctggact gtacgctggg gaaatcaggc agatcaacaa
      721 tgtcagtttg cttttagtac accggattca gaaccaacaa cctctgtatt acaaggcaca
      781 gcgcagtgcc attaaggata aaaaaatgaa aagagcgcct cttataacag gacttttgtt
      841 gatatccaca tcctgcgctt atgcctcctc aggagggtgt ggagccgaca gcactagcgg
      901 tgcgacaaat tacagcagtg tggttgatga tgttacggtg aaccagacag ataacgtgac
      961 aggacgggag tttacctctg caacgctaag tagcactaac tggcaatacg cctgttcctg
     1021 ctctgcgggt aaggcagtta aacttgtcta tatggtcagc cccgtactta ccaccactgg
     1081 acatcagaca ggatattaca aactcaatga cagcctggat attaaaacca cattacaggc
     1141 aaacgacatt ccaggactca caaccgacca ggttgtctct gttaacaccc gattcacaca
     1201 gataaaaagc agcaccgtat attctgctgc aacccaaacg ggtgtttgcc agggtgacac
     1261 gtctcgttat ggacccgtta atattggtgc aaataccacc tttaccctgt atgtcaccaa
     1321 gccatttctc ggctcgatga ccattccgaa aacggatatt gccgtcatta aaggcgcgtg
     1381 ggtcgatgga atgggaagcc cgtctacagg tgacttccat gatttagtca agttatcgat
     1441 tcagggaaat ctcaccgccc cacagtcgtg caaaattaat cagggcgatg ttattaaagt
     1501 taattttgga ttcatcaatg gtcagaagtt taccacccgc aatgccatgc cagacggttt
     1561 tactccagta gactttgata tcacttatga ctgtggtgat acttcaaaga ttaaaaactc
     1621 gttgcaaatg cgcatcgacg gtacaactgg ggtagtagac cagtacaacc tggtcgccag
     1681 gcgaagaagt tcagacaatg tgcccgatgt cggtattcgt attgaaaatc tcggcggcgg
     1741 agttgcaaat attccttttc agaacggtat ccttcccgtt gatccttccg ggcatggcac
     1801 cgtcaatatg cgcgcctggc cagttaatct ggtcggtggt gagctggaaa caggaaaatt
     1861 tcagggcaca gccgccatta ccgtcatggt gcggtaaaag aagacgttac gttttgatta
     1921 acgcaacgct tcggcactgt tacccctgct gctccagcgc atacttatac agcgcatttt
     1981 tcttcacgcc atgaatttct gcggccagcg ccgccgcttt tttcagcggc agttctgcct
     2041 gtagcagcgc cagcgtgcgc agggcatcgg cgggtaagtc ttcttcctgt gctttatgac
     2101 cttcgacaat cagcaccatt tcgcctttgc gacggttttc atcttccttt acccacgcca
     2161 gcagctcgcc aacgggcgcg ccgtgaatgg tttcccaggt tttggtcagc tcacgcgcca
     2221 gaaccacgta gcgggattcg cctaataccg caacgatatc ttccaggcta tctaacagac
     2281 ggtgggtaga ttcataaaaa atcagcgtgc gcggctccgc ttcaatggct tttagcgcat
     2341 cacggcggcc ttttgatttg gcaggtaaaa agccttcgta acagaaacgg tcagatggta
     2401 aacccgcggc gcttaacgca gtgatagcag cacacggtcc gggtagcggc accacacgaa
     2461 tccccgcttc acggcaggta cgcaccagat ggtagccagg atcgttaatt agcggcgttc
     2521 cggcatcgga aaccagcgca atgttttgcc cctcttgcag ctttgccagc agcgtttcgg
     2581 ctttttgttg ttcgttatgg tcgtgcagcg caaacaaccg ggcattaatc ccaaaatgtt
     2641 gcagcaataa accggtgtga cgagtatcct cggcggcaat cagatcaacg gcctgtaata
     2701 cctctaacgt acgctgggtg atatccgcca gattgccgat tggcgtcggt acaatgtaaa
     2761 gctggccctg agaattatcc gccgattggt gttgtttcat tgtgtcgtcc gtattgccga
     2821 tttaatattg agcattgcgt aaaaaaaata tcactggata cattatggta ccctcaacat
     2881 tttctcgttt gaaagccgcg cgttgtctgc ctgttgttct ggcagccctg attttcgccg
     2941 gttgtggcac ccatactccc gatcagtcca ctgcttatat gcagggcacg gcgcaggctg
     3001 attctgcctt ttatcttcag cagatgcagc aaagctctga tgataccagg atcaactggc
     3061 aattactcgc cattcgtgca ctggtgaaag aaggtaaaac cgggcaggcg gttgagttgt
     3121 ttaaccaact accgcaagaa ctgaacgatt ctcagcgtcg cgagaaaaca ctgctggcgg
     3181 cagagattaa actggcgcag aaagattttg ctggcgcgca aaacttgctg gcgaaaatca
     3241 cacctgccga tttagaacaa aaccagcaag cgcgttactg gcaggcaaaa atcgatgcca
     3301 gccaggggcg tccttccatt gatttactgc gcgcgttaat tgctcaggaa ccgctgcttg
     3361 gcgcgaaaga aaaacagcag aatattgatg ccacctggca ggcgctctcc tccatgactc
     3421 aggaacaggc gaatacgctg gtgatcaacg ccgacgaaaa tattctgcaa ggctggctgg
     3481 atctgcagcg cgtctggttt gataaccgta acgatcccga catgatgaaa gccgggatcg
     3541 ccgactggca gaaacgttat ccgaacaatc cgggcgcgaa aatgctgcca acgcagttgg
     3601 ttaacgtaaa agcgtttaaa ccagcctcga ccaacaaaat cgccctgctg ttgccgctga
     3661 atggccaggc agcggtattt ggtcgcacta ttcagcaagg ctttgaagcc gcgaaaaata
     3721 tcggcactca gccagtggcg gctcaggtag ctgccgcacc tgccgcagac gtagcagaac
     3781 aacctcagcc gcaaactgcg gatagcgttg ccagcccggc acaagcctcg gttagcgatc
     3841 tgaccggtga tcagcctgcg gcccagccgg tgcctgtaag cgccccggcg acaagcaccg
     3901 cagcggtaag cgcacccgca aatccatccg cagagctgaa aatctacgat acctcatcac
     3961 aaccacttag ccagatctta agccaggttc agcaggatgg cgcgagtatt gtggttggtc
     4021 cgttgctgaa aaataacgtt gaagagttgc tgaagagcaa caccccgctg aacgtactgg
     4081 cactgaacca gccggagaat atcgaaaacc gcgtcaatat ttgttacttc gcgctttcac
     4141 cggaagacga agcgcgcgat gcagcgcgtc atattcgtga ccagggtaaa caagcgccgc
     4201 tggtgctgat cccacgcagt gcattgggcg atcgcgtagc caatgcgttt gcgcaagagt
     4261 ggcagaaact gggcggcggc accgttctgc aacaaaaatt tggttccacc agcgaattac
     4321 gcgcgggtgt taacggcggt tctggtattg ctttaacggg tagcccgatt actcccagag
     4381 cgacaaccga ctccggcatg acgaccaaca atccaacgct gcaaaccacg ccaaccgatg
     4441 accagttcac caataatggc ggtcgtgtcg atgcggtcta cattgtggca acgccgggtg
     4501 aaatcgcttt tatcaaaccg atgatcgcca tgcgtaacgg tagccagagc ggtgcaacgc
     4561 tgtacgccag ttcccgcagc gcacaaggca ccgcaggccc ggatttccga ctggagatgg
     4621 aaggcttgca gtacagcgaa atcccgatgc tggcaggcgg taatctgccg ttaatgcagc
     4681 aggcgctcag cgcggtgaac aacgattatt cgctggctcg catgtatgcg atgggcgttg
     4741 atgcctggtc gttggcaaat catttctcac aaatgcgcca ggttcagggt tttgaaatca
     4801 acggtaatac cggaagtctg acggctaacc cggattgcgt gattaacagg aagttatcat
     4861 ggctacagta ccaacaaggt caggtagtcc ccgccagtta accaccaaac agaccggcga
     4921 tgcgtgggaa gcacaagcgc gtcgctggct ggaaggcaaa ggactgcggt ttatcgccgc
     4981 taacgtgaac gagcgtggcg gcgagatcga tctgataatg cgtgaaggcc tgaccaccgt
     5041 ttttgtcgag gtacgctatc gccgctctgc gctttatggc ggcgcggcag ccagtgtgac
     5101 ccgcagcaaa caacacaaat tattacagac tgcccgcttg tggctcgcgc gtcataatgg
     5161 gagttttgat actgtggatt gccggttcga tgtggtagcc ttcaccggga atgaggttga
     5221 gtggattaag gatgccttta atgaccactc ataattaagg tttaaggatt agcgtgcaag
     5281 aaagaattaa agcttgcttc actgaaagca ttcaaactca aattgcggcg gcagaggcgc
     5341 ttccggatgc catctcccgt gcagccatga cgctggttca gtctctgctc aatggcaaca
     5401 aaatcctctg ttgtggtaat ggaacttccg ctgccaatgc acagcatttt gctgccagca
     5461 tgatcaaccg tttcgaaacg gagcggccca gcttacctgc cattgcacta aatactgata
     5521 atgttgtctt aacggcgatt gccaacgatc gcttacatga tgaagtgtat gcaaaacagg
     5581 tgcgggcgct gggtcatgcg ggagatgtat tgttagccat ttccacccgt ggcaacagcc
     5641 gcgatattgt taaagcagtt gaagccgccg ttacgcgtga tatgaccatt gtggcattga
     5701 ccggctatga cggcggcgaa cttgcaggtt tgttagggcc acaggatgtg gagatccgca
     5761 ttccttcgca tcgtagtgct cgcattcagg aaatgcatat gctgacggta aattgcctgt
     5821 gcgatctgat cgataacacg cttttccctc accaggatga ttaaggagaa tacatgaagg
     5881 cattatcgcc aatcgcagtc cttatttccg cgctgctgtt gcaaggttgt gttgccgctg
     5941 ccgtagtggg taccgctgct gtgggtacca aagccgcaac tgacccacgc agtgtcggca
     6001 cccaggtgga cgatggtacc ctggaagtgc gcgtgaacag cgcattgtcg aaagacgaac
     6061 agattaagaa agaaacccgt attaatgtca cggcctatca gggcaaagtg ctgctggttg
     6121 ggcagtcacc aaatgctgaa ctttcggctc gcgccaaaca gattgctatg ggcgtagacg
     6181 gtgccaacga agtgtataac gagattcgtc agggccagcc gattggtctg ggcgaagcat
     6241 ctaacgatac gtggatcacc accaaagtgc gttcgcagct cttaaccagc gacctggtga
     6301 aatcgtccaa cgtgaaagtg accaccgaaa acggtgaagt gttcctgatg gggctggtga
     6361 ctgaacgtga agcgaaagcg gcggcagata ttgccagccg ggtgagcggc gtaaagcggg
     6421 ttaccacagc gtttacgttt attaaatagc agccccttgt aatgcctgat gcgacgcttg
     6481 ccgcgtctta tcaggcctac aacgatgcaa accgtaggtc ggataaggcg tttacgccgc
     6541 atccgacatg ttttccctca aatcagaaca acagcgccag cccgccgaca atcacaccag
     6601 acactgccac catcgcccct gtcagccata aggctttcgc cgggaacgct ttgcgcagca
     6661 taatcagtga cggcaaactc actgccggga gcgtcatcaa caatgccagc gccggagcgg
     6721 ttcccatacc tgccagcatc atcgtttgta caatcggaat ttctgctgcc gtgggaatga
     6781 caaacaagca tcctgctacc gccatcgcca ccacccacat caggctgtta tcgacagcac
     6841 catcggcatg ggggaataac cagacgcgtg cggcacccaa caccagtact gcaaggatgc
     6901 aaaccgggat cgtactccag aaaagtgtcc atagcgccct gccccagcgg ctaaaaaaac
     6961 cgctctgtgc ttccggtatg tcaatttcga ccggtgcctg cgtttgcagt gtttcacgca
     7021 cccatttttg caccagcgtc gcaatcagca acgccatcac cagcccggcc accagacgaa
     7081 tcgccgcaaa accccagccg aggacaaacc ccataaacac cagcgtcgcc gggtttaaca
     7141 ccggattgcc catccagaat gccagcgcac cgcccatcga tacctgttgg cgacgcattc
     7201 ccgccgcgac cggagccgca cagcaggtac acatcatgcc cggcagcgaa aacagcgttc
     7261 ccagcagcgt gccgcgaaag cgcgattgcc caagcgtacg caacaaccag tcacgcggga
     7321 tcagcacctg aatcaacgaa ccgaggatca cccccagcac cgccgctttc catactgcga
     7381 ggaaatagat catcgcgtaa tccaacgccg cttgccacgg attagcatcc gcctgcgcaa
     7441 ggatagattt accgatactg tgggtttcgg ctgcagtaaa cgctttgccg tagtaaggtt
     7501 gccatttcac ataccagaga ccggcaatga caacgagaaa gaaaagcgcg ggtttccacc
     7561 actgaatggg cgttgccgcc tgagatgaag actgaccagt catagcattc cccaggaata
     7621 agttatgata attgagcgcg tgaatattac gctcactatc aattcttggg aataattatt
     7681 cagctctttt gcgtaattct gaagagctta aaatcgtcac gccttcatgc tcaggccgca
     7741 tcgcctctgc cagcatgacc cgcgccacat ctctcgcatc aatggatttc cagttacctg
     7801 gtaacaaacg gaacagcggc gcaaaaagcg tttcgttcat ccgctgtttg ctacgatcgc
     7861 ccagtaacat cgacgggcga gcaatggtca atttcggcca gttctgggca attaatgctt
     7921 cttccatctc ccctttgacg cggttataga aaaacggcga gtgggcattg gcacccatag
     7981 cactgaccac cagcatatgc tgcgcgccca gtcgccgccc ggttaatgcg gtatccacta
     8041 ccagcgtgta atcggcatga ataaacgcct ctttgctccc cgcttctcgc cgcgtggtgc
     8101 cgagacaaca aaacacaatg tcgatgggtt cggtgacctg cgccaacgcg tcactcagtt
     8161 gcggatcatg gggattaaac accccgggca tatcgcccaa cggacgtcgc gtcggcgcag
     8221 caatggcgtt aactttcggt tcattaatca acatccgcag caggtgaccg cccaccagcc
     8281 ccgttgcgcc tgtaatcagt acctgactca tcttcgctcc tttacagaat tgtctgcctt
     8341 gcgctccccg gctcatgcac cagtcttaat agaccatgag gtaattatcc ccgattgtgg
     8401 aaaattcgcc tcatccaatg caacaacgcg gaggaagcat gagtaagaaa attgccgttt
     8461 taatcactga tgaatttgaa gattcagaat ttacttcacc cgcagacgag ttccgtaaag
     8521 ccggacacga agtgattact attgaaaaac aagcgggtaa aacggtgaaa ggcaaaaaag
     8581 gagaagccag cgtgaccatc gataaatcca tcgatgaagt gacgccagcg gagtttgatg
     8641 ccctgttgct accgggtggt cattcaccgg attatctgcg tggcgacaac cgttttgtca
     8701 cctttacccg tgattttgtg aatagcggca aacctgtttt cgccatctgt cacggtccgc
     8761 agctactgat cagcgccgat gtgattcggg ggcgcaaact gaccgcagtt aaaccgatca
     8821 ttattgatgt taaaaatgcg ggcgtggaat tttacgatca ggaagtcgtg gttgataaag
     8881 atcagctggt taccagccgg acaccggacg atctgccagc gtttaaccgc gaggcgttac
     8941 gcctgctcgg tgcctgagtc gcgtagccaa atcatttttt taccaaagcc cagcgtgttg
     9001 tcggtgaatt tgatttcatc gagccggatt tcccacaccg gggccgacag cattctggca
     9061 accggaaagc gacgattgta cgccttgcgc gcgaggtcgc tttcctcacc ttccagcctg
     9121 cggatctcac ctttaaactg cacaccgcga attaacgcta ccgttttcgg ctgaccgttt
     9181 accgttccgg caaccgcagc ctgcggccca ctcatctgcg cgtggcgcgt tttttcttcc
     9241 gtcaaaatgt agaaggcgac tttctgcgca tcaaaaagat aaaaggcatt agcgcaccag
     9301 agttcccctt cctgctgcac acaccaggtg acaacatgtt gttttgccag ccaacggctg
     9361 atggcgatga gtgtttccat tgctgttctc ttttatactg tgggcctgac cttaacatgc
     9421 gatgcagacg atgacaccct ggtttcttta cttgatccgt accgccgaca ataagcttta
     9481 taccgggatc accacggatg tcgaacgccg ctatcagcag caccaaagcg gcaaaggggc
     9541 gaaagcactg cgcgggaaag gagaactgac gctggcgttt tctgcgccag tgggcgaccg
     9601 ttcgctggca ctacgggcgg aatatcgcgt taagcaactg accaaacggc aaaaagagcg
     9661 tctggtagcg gaaggcgcag ggtttgcaga actgttgagc agcctgcaaa ccccggagat
     9721 taaaagcgat tgaaatgctc gtgatactca accagaccgg ttacgccatt cagcgcgtca
     9781 tccgctaaac gatgtaccag gaaggcgctt tcagtgcccg gccagcggca acgcagatcg
     9841 tgatgcgccg ccagttcaaa gccgaaacga ctgtacagcg ccggatcgcc cagcgtcacc
     9901 actgcggcat agccgaactc attaagcgaa tcgagtcctt catagaccag ttggcgtgcc
     9961 agcccctgtc cacggtattt ttcatcaacc gccaacggtg ccatgccgac ccattgcagg
    10021 tcttcgccct gcacatcaac cggactaaat gccacatagc caatgacctg accttcgtca
    10081 tctgtcgcca ccagccccag cgtcagaaag ccatcttcac gcagatcgtg aaccagcttc
    10141 gcttccgcat cactttcgaa tgagcgacgc agcagggcat caatacccgg cgcatcaatg
    10201 ggaatttcta ctcgaattag catggttcac ctaccgatgt ctgtttggtt tcaggcgcgg
    10261 ttttcattcc cgcctcaaca aaatccgcca gttgcagcag catcatgcgt aaggctttcg
    10321 gcatttgctc cagttcaatg gcgtccatca ggtttttcac atacaccccc agctccgtat
    10381 cgccttcaat caccagccga cgctggaaga agagcgtatc cggatcttgt ttacgcgccg
    10441 caatcatcag cagatcgctg gcgtcggcac taaaactcac atcagcttgc gtgttctggc
    10501 tgaccaccag tttgccattc accaccgagg taaaccattg caggtcaata tcacgcacat
    10561 gaatacttaa ccagcggcct tcaagaaact ccagctcgcc atcatccagc gcctgacgga
    10621 attgccagct taagacctgc tcaagaacct ggcgttttag cgcaaatggc gtcagtttta
    10681 ccggtacact caacagagat ggccccaaat gcacaatacg ggaacgcagt ttatccaaca
    10741 cgagttttac tccctgtttc aacaatcatc ctattttgcc atatcagaaa aataacatag
    10801 cggtataaat caacaattcc atatgaaatt gctgctacca ccaatacaac tttaactgcc
    10861 ttaaatcaaa aattgtcgca gcaaggttaa ctaaaatccc agttcgttaa catttttgcg
    10921 ttttgatagc gcaaccttca ggaaaaatta tggagctgct ctgccctgcc ggaaatctcc
    10981 cggcgcttaa ggcggccatc gaaaacggcg cagatgctgt ttatatcggg ctaaaagatg
    11041 ataccaatgc ccgtcacttc gccggcctta actttaccga gaaaaaattg caggaagcgg
    11101 tgagttttgt ccatcaacat cgccgcaaac ttcacatcgc gattaacact tttgcgcatc
    11161 cggacggtta cgcccgttgg cagcgtgccg tggatatggc ggcgcagctg ggtgccgacg
    11221 cgctgatcct cgccgacctc gccatgctgg agtacgccgc cgagcgttac ccgcatattg
    11281 aacgtcatgt gtcagtgcag gcttcggcga ccaatgaaga ggcgattaac ttttatcatc
    11341 gccattttga cgttgcccgc gtggtgctgc cgcgcgtgtt gtcgattcat caggtgaaac
    11401 agctggcacg ggttactcct gtcccgctgg aagtgtttgc tttcggcagc ctgtgcatta
    11461 tgtcggaagg ccgttgctat ctgtcgtcgt atctgacggg tgagtcgccc aacactgtgg
    11521 gtgcgtgttc tccggcccgt ttcgtgcgct ggcaacaaac gccgcagggg ctggaatccc
    11581 gcctgaacga agtgctgatc gaccattatc aggacggcga aaacgcaggt tatccgacgc
    11641 tgtgtaaagg gcgttatctg gtggacggcg agcgctatca cgcgctggaa gaaccaacca
    11701 gtctcaatac cctggaactg ctgccggagt taatggctgc gaatattgct tcggtgaaaa
    11761 ttgaaggccg ccagcgtagc ccggcgtatg tcagccaggt ggcgaaagtc tggcgtcagg
    11821 ctatcgaccg ttgtaaggcc gatccgcaaa acttcgtacc gcaaagcgcg tggatggaga
    11881 cgctcgggtc gatgtccgaa ggcacgcaaa ccacccttgg cgcgtatcac cgtaaatggc
    11941 agtgagaaga gcaatgaaat attccttagg gccagtgctg tggtactggc caaaagagac
    12001 gctggaagat ttttatcagc aagccgccgc cagcagcgcc gacgtgattt atcttggtga
    12061 agcggtatgc agcaagcgcc gggcaaccaa agttggcgac tgtctggaga tggcaaaatc
    12121 gctcgccgga agtggcaagc agattgtgct ctccacgctg gcgctggtgc aggcatcctc
    12181 tgaactgggc gaactgaaac gctatgttga gaacggtgaa tttctgattg aagccagcga
    12241 tctcggcgtg gtgaatatgt gcgctgaacg caaactgccg ttcgtcgccg ggcacgcgct
    12301 gaactgctac aacgcggtga cgctgaaaat attgctcaaa cagggcatga tgcgctggtg
    12361 tatgccggtg gagctttccc gcgactggct ggtgaatctg cttaatcagt gcgatgagct
    12421 gggcattcgc aaccagtttg aagtggaagt cctgagctac ggtcatctac cgctggccta
    12481 ctccgcccgc tgctttaccg cgcgttcgga agaccgcccg aaagatgagt gtgaaacctg
    12541 ctgcattaag tatccgaacg ggcgcaacgt gctgtcgcag gaaaaccaac aagtgtttgt
    12601 actcaatggc attcagacca tgagcggcta cgtttacaac ctcggtaacg agctggcatc
    12661 catgcaggga ttggttgatg tagtccgcct gtcaccgcag ggcactgaca ctttcgcgat
    12721 gctcgacgcc ttccgcgcta atgaaaatgg cgctgtgcca ctgccgttga cggcaaacag
    12781 cgactgtaac ggctactggc ggcggctggc aggactggag ctgcaagcct aagtaaatag
    12841 ctcactttgt taacaacttt aactattctt taatgcagta ttaaagatta atcggtaaca
    12901 aagtgagctg ttatgactga taaaaccatt gcgttttcgc tactcgatct ggcccccata
    12961 cccgaaggct cttcagcgcg ggaagcgttc tcccactctc tcgatctcgc ccgtctggct
    13021 gaaaagcgcg gctatcatcg ctactggctg gcagaacacc acaatatgac cggcattgcc
    13081 agtgctgcaa catcggtatt gattggctat ctggcggcga ataccaccac gctgcatctg
    13141 gggtctggcg gcgtgatgtt gcctaaccac ttaccgttgg tcattgccga acagttcggc
    13201 acactcaata cgctctatcc ggggcgaatc gatttggggc tgggtcgtgc tccgggtagt
    13261 gaccaacgga caatgatggc gctacgtcgt catatgagcg gcgatattga taatttcccc
    13321 cgcgatgtcg cggagctggt ggactggttt gacgcccgcg atcccaatcc gcatgtgcgc
    13381 ccggtaccag gctatggcga gaaaatcccc gtgtggttgt taggctccag cctttacagt
    13441 gcacaactgg cggcgcagct tggtctgcca tttgcgtttg cctcacactt cgcgccggat
    13501 atgttgttcc aggcgctgca tctttatcgc agtaacttca aaccgtcggc tcggctggaa
    13561 aaaccatacg cgatggtgtg catcaatatt atcgccgccg acagcaaccg cgacgctgaa
    13621 tttctgttta cctcaatgca gcaagccttt gtgaagctgc gccgtggcga aaccgggcaa
    13681 ctgccgccgc cgattcaaaa tatggatcag ttctggtcac cgtctgagca gtatgacgtg
    13741 cagcaggcgc tgagtatgtc gttggtaggc gataaagcga aagtgcgtca tggtttgcag
    13801 tcgatcctgc gcgaaaccga cgctgatgag attatggtta acgggcagat tttcgaccac
    13861 caggcgcgcc tgcattcgtt tgagctggcg atggatgtta aggaagagtt gttgggatag
    13921 tgtgtcttaa cgcgggaagc cttatccgag ctggcaacgc tgtcctacgt aggcctgata
    13981 agcgaagcgc atcaggcatt gtgtaggcag cagaaatgtc ggataaggca ccgctgatta
    14041 ctgatacacc ggcagtaaat taaagctcga taaaatatgc accagtgcgt tgccgacgcc
    14101 aaacaccaga atcagcgcaa tcatcggctt gccaccccag acgcggaatt tcgggctgcc
    14161 aaagcgttta cgcgatgcgc gggccagcag tgccggaaca attgccgccc agatggtagc
    14221 cgctaaacca gcataaccaa tggcatacag gaatccgttc gggaacagca gaccacccac
    14281 aacaggcggg gcaaaggtca gcaatgccgt tttcaagcgg cccacagccg agtcgtcgaa
    14341 accaaacaga tctgccagat agtcaaacaa acccagcgtt acgccaagga acgaactcgc
    14401 taccgcaaag tttgagaaca cgaccagcag aaggtccagg ctacggctgt tcagcacgcc
    14461 gcttaatgct tgtaccaaca catcaatatt accgcccttc tcggcgatgc cgataaactc
    14521 cggacgaggg atgttgccca ttgtcgccag caaccagatg gtatacagcg ccagtgccat
    14581 cagcgtaccg tacaccaggc atttcacgat ggttttcgga tctttgccgt aatacttcat
    14641 caggcttggc acgttaccgt gataaccaaa cgacgccagg cagaacggca gggtcatcag
    14701 caaatacggc gcataagatg cattgctttc ggcaacgttg aacaatgtcg caggctgcac
    14761 atgccccagc aggctaccga aggtgaggaa gaaggtaatg actttcgccc ccagcacaat
    14821 cgccgtcatg cggctgacgg ctttagtact caaccacacc acaaacgcta ccagcagtgc
    14881 aaaaccaaaa cccgccgccc gtgccgggac gtttagcgac atctctgcga aggtgtgatg
    14941 cagaatcgaa ccactggcag aaatataggc ataggtcagg atatagagca caaaggcaat
    15001 ggaaatgccg ttgaccacgt tccagccttt gcccagcaaa tctttggtga tggtgtcaaa
    15061 actcgaaccg attcggtaat tcaggttagc ttccagaatc atcaagccgg aatgcagcat
    15121 acagaaccag gtaaagatca gcgctgccat tgaccagaaa aaccacgccc cggacatgac
    15181 cactggcaga gaaaacatcc ctgcgccaat aatggtgccg ccgataatca ccacgccgcc
    15241 aagcagcgac ggtgacgttt gggtggtggt tcgtgttgcc atgagggctt ctctccagtg
    15301 aaaaatagtg cgactgcgtt gttatgcatt acactgtacc agtacatgag tacaaaagac
    15361 agaaaaaaag ccccgatggt taaaatcggg gctgtatata ttattttaca gattgtgttc
    15421 gctgttcagc gatgattacg catcaccacc gaaacgacga cgaccggtag agtcatcacg
    15481 acgcggtgcg cggccttcac gacgttcgcc gctaaaacga cgaccatcac cacgaccacc
    15541 ttcacggcgt tcaccgctga agttacgacc gccttcacga cgctcgccac cgaaaccacg
    15601 accaccgcca cgacgctcac cgccagtatg cggctgtgca tcgcccagta actgcatgtt
    15661 catcggcttg ttgagaatgc gagtgcgcgt aaagtgttgc agcacttcac ccggcatacc
    15721 tttcggcagt tcgatggtgg agtgagaagc aaacagcttg atgttaccga tgtaacggct
    15781 gctgatgtcg ccttcgttag caatcgcgcc aacgatatga cgaacttcaa caccatcatc
    15841 gcggcccact tcaatgcggt acagctgcat atcgccaaca tcacgacgtt cacgacgcgg
    15901 acgatcttca cggtcgccac gcgggccacg gtcgttacga tcgcgcggac cacggtcatc
    15961 acggtcacgg aattcacgtt tcggacgcat cggcgcatct ggcggaacaa tcagagtacg
    16021 ttcaccctgt gccattttca gcagtgccgc agccagagtt tcgagatcca gctcttcacc
    16081 ttctgcagtc ggctgaattt tgctcagcag tgcgcggtat tgatccagat cgctgctttc
    16141 cagctgctgc tgtactttag cggcgaattt ttccagacgg cgtttgccca gcagttctgc
    16201 gttcggcagt tctacttccg gaatagtcag cttcatagta cgttcaatgt tgcgcagcag
    16261 acgacgctcg cggttctcaa cgaacagcag cgcgcggcca gcacgacccg cacgaccggt
    16321 acgaccgata cggtgaacgt aagactcaga atccatcggg atatcgtagt taaccaccag
    16381 gctgatacgc tcaacgtcca ggccacgggc cgcaacgtcg gtcgcaatca ggatgtccag
    16441 acgaccgtct ttaaggcgtt ccagtgtctg ctcacgcagc gcctggttca tgtcaccgtt
    16501 cagcgcggcg ctattgtagc cgttacgctc aagagcttca gccacttcca gagtcgcgtt
    16561 tttggtacga acgaagataa tcgccgcatc aaaatcttcc gcttccagga aacgtaccag
    16621 tgcttcgttt ttacgcatac cccacacagt ccagtagctc tggctgatgt caggacgggt
    16681 agtcacgctg gactgaatac gcacttcctg cggctctttc ataaagcggc gggtaatgcg
    16741 acgaatcgct tccggcatgg ttgcagagaa cagagcggtc tgatgacctt ccgggatctg
    16801 cgccataatg gtttcaacgt cttcgatgaa gcccatgcgc agcatttcgt cagcttcatc
    16861 cagaaccaga ccgctcagtt tagagaggtc cagagtgcca cgtttcaggt ggtccagcag
    16921 acggcccgga gtaccgacaa cgatctgcgg cccctgacgc agggcgcgta attgcacgtc
    16981 ataacgctgg ccgccgtaca gggcaaccac atttacgccg cgcatgtgtt tagagaaatc
    17041 cgtcattgct tcagcaacct gtaccgccag ttcgcgggtc ggtgccagca ccagaatctg
    17101 tggtgctttc agctcaggat caagattctg cagcagaggt aaagagaatg ctgcagtttt
    17161 tccgctcccc gtctgggcca tacccagaac gtcgcggcca ttcagcagat gtggaataca
    17221 ctctgcctga attggagatg gtttttcgta acccagatcg ttaagggctt caaggatagg
    17281 agccttcagg cccagatctg caaaagtggt ttcgaattca gccatgtagt acgtgtgcct
    17341 caaaattaat ggcggccagt ctacataact catcatgaaa ttgatcagca attttcattg
    17401 aaaagtgtga accggctcaa agtaggtgta ttaacgaaca acaacgccct cacccgttaa
    17461 ggtgatggca atcaaaaaag attacgggct gatgtgtacg tcagctattg ctggtccgat
    17521 tctgccaggt catcttggtc ctggcccagg agcgataatt ccaacaatgc gtatcggtgc
    17581 tcaacaaagt tatgaacgtt gttggcaacc gccagtttga acagtgccgt ggcgctgtcc
    17641 aaatccccca gacttaggta gtacttacct aaatagaagt tggtttcact gagatgctca
    17701 gcgagcgagg tgttatccgt tgcgtccgcc ttgagccttt ccattaacgt ttgttcgcta
    17761 atgttgccca ggtagaactc gacaatgttc catccccact gttccttatc cgatttttcg
    17821 aagtgctgtt tcaatacttc tttagcctgc ttctcatcga gcttctgctc ggcgagataa
    17881 agccacagac tacggaaagg atcattggga tcgtcttgat aaaacgccag cagatcatct
    17941 tgcgctaact tgtcacgacc gccgtaatat aatgcaatcc cgcgattcaa gtgcgcgtag
    18001 ttgtaagttg gatcaagctc aagtacagaa tcaaacgctt cataggcagc atcaaaattg
    18061 cctgcctgcg ttaaatatat gcctaagtaa ttgaatactt caggcatatc cggtcggatt
    18121 gccagcgctt gcgaaaaatc gttacgcgct aatgccctca gaccgagact atcatacaac
    18181 actccgcgct catataaaag ctgtgcgcgt tcgtcatcgg ttaaagcccg actggcaagg
    18241 atttgttcca tacgtgccag aatcacttcc tgctgtaaag tcggttgcaa tggtaccgcg
    18301 aggacttcac ttttacgcca ggaagtatta ctgcatcctg caagcgtaag tgctgtcgca
    18361 acgaaacacc agcgcaaaaa aggcttcatt tcccactccc gaagaccacg gttgaatgaa
    18421 cgtcctgttc ccggttgcta acaaggcgtc ctgcccggtt aaaagccccc cgccgcagcg
    18481 gagggcaaat ggcaacctta ctcgccctgt tcagcagccg gagcttccgg tgctgcagca
    18541 ggttgagact gctcagtcgc ttctttaatg ctcagacgga tacggccctg gcgatcaact
    18601 tccagaactt tcaccggtac ttcctgaccc atctgcaggt aatcggtcac tttctcaacg
    18661 cgtttgtcag cgatttgaga gatgtgtacc agaccttctt taccgccgcc gatggcaaca
    18721 aatgcgccaa agtcaacgat acgggtcact ttaccagtgt agacgcggcc cacttcgatt
    18781 tctgcagtga tctcttcgat acgacgaata gcatgtttcg ctttctcgcc gtcggtcgct
    18841 gcaatcttca cagtaccgtc atcttcgatt tcgatggtgg tgccagtttc ttcggtcagg
    18901 gcacggataa cagagccgcc tttaccgata acgtctttga tcttgtccgg gttgatcttg
    18961 atggtatgga tacgtggtgc gaactcagag atatcgccac gcggcgcgtt gatcgcctgt
    19021 tccattacgc ccaggatatg cagacgcgca cctttagcct ggttcagcgc aacctgcatg
    19081 atctctttgg tgataccttc aattttgata tccatctgca atgcagagat accctcgcgg
    19141 gaacccgcaa ctttgaagtc catatcgccc aggtgatctt cgtcgcccaa aatgtcagac
    19201 agtacaacgt agttgtcgcc ttctttcacc agacccattg cgatacccgc aacggcagct
    19261 ttgatcggca cacctgcgtc catcagcgcc agagacgcgc cgcacacgga agccatagaa
    19321 gaggaaccgt tggattcagt gatttcagac acaacacgta cggtgtacgg gaatttgtcc
    19381 atatccggca tgactgccag cacgccgcgc ttcgccagac gaccgtgacc aatttcacga
    19441 cgcttcggag aaccgaccat gccggtttcg cctacggagt acggagggaa gttgtagtgg
    19501 aacaggaagg tatcggtacg ttcgcccatc agttcatcaa gaacctgcgc gtcacgagca
    19561 gtacccagcg ttgcggtaac cagcgcctgc gtttcaccac gggtgaacag cgcagaaccg
    19621 tgagtacgcg gcagcacgcc agtacgcaca tccagaccac ggatcatatc tttttcacga
    19681 ccgtcgatac gcggttcgcc tgccagtacg cggctacgaa caacgttttt ctcgatcgcg
    19741 tgcagaattt cacccagttc gttttcgtcc agggtttcgt cttcagcaag cagcgtcgcg
    19801 atggtttcag atttgatgac atcaacctgc gcataacgct cttgtttgtc ggtgatgcgg
    19861 taagcatcgc tcaggcgagc ttcagccagt gcagcaacgc gcgcgttcag cgcttcgttt
    19921 accggctccg gctgccagtc ccaacgtggt ttaccggctt ctttcaccag ttcattgatg
    19981 ttctgaataa caacctgctg ttgttcatga ccgaacacta ctgcgcccag catctggtct
    20041 tcgctcagca gttcagcttc agattcaacc atcagtacag cggcttcagt accggcaaca
    20101 accagatcca gtttgctctc tttcaactcg tcctgagtcg ggttcagtac gtactggtca
    20161 ttgatgtaac ctacgcgagc agcaccaatc ggaccattga acggaatacc agacagagac
    20221 agtgctgcgg aagcaccaat catcgcgacg atatccgggt taacttgcgg gttaacagaa
    20281 accacggtgg cgataacctg aacttcgttg acgaagcctt ccgggaacag cggccgaatc
    20341 gggcggtcaa tcagacgcgc gatcagggtt tcgccttcgc ttgggcggcc ttcacgacgg
    20401 aagaagctac ccgggatacg accagcagcg taggtacgct cctgatagtt aacggtcagt
    20461 gggaagaagt cctgacctgg tttggctttt ttctggccaa caacggtaac aaataccgcg
    20521 gtgtcatcca tgctaaccat aacagcggca gtagcctgac gagccatcat gccggtttcc
    20581 agagtcacgg tgtgttggcc gtactggaat ttacgaacga tcggattaag caatgtaata
    20641 tcctttctct ttcttagaca gtaccttacg gcactggtgt taatacccga tcttctgcgc
    20701 atcctcgcga ctaatgacaa ccctaaccca gctctatgtg ggtaaagcct ctcattagcc
    20761 gcgcgaacct ctgcaacgga agatcattca tagcaacaat acattagttt ccagtgaatt
    20821 gctgccgtca gcttgaaaaa aggggccact caggccccct tttctgaaac tcgcaagaat
    20881 tagcgacgca gacccaggcg ctcgatgagc tgggtgtaac gtgctacgtc tttacgtttc
    20941 aggtagtcga gcagtttacg acgctgagaa accatgcgca gcagaccacg acggctgtgg
    21001 tgatcttttt tgtgctctgc aaagtggccc tgcaggtggt tgatctgtgc agtcagcagt
    21061 gctacctgaa cttcagtaga accggtgtcg tttgcgtcac gaccaaactc agaaacgatt
    21121 ttagctgttg cttcagtact tagagacatt ttaaaactcc aaagtatata gaatgaaagg
    21181 acgccgatct ctaattcagc gatcccagtg tacgttacgc aaagtgttaa acaatttacg
    21241 cgacgttaag cggcagtatt ctactcgtag cgacctgtta tcgcaagacg gttaacatta
    21301 cgccgggtat tcaaccacca ggcgacgagg cgcaacgcgg ccttcatcgt caatttcgcc
    21361 cataccgata aatttgccgt tctcaccttc tgtaacgcga accagtcctt ccagcggcgc
    21421 accggatgta cgaactgggt taccattttt gaagtaaaca gaagacgtta acggaagatt
    21481 caccaccggg tagtccgaag ctggactgtc cattggcatc agtaatggat caagtaactc
    21541 cgcagccgga atatcctgct gttcggcttg ttcgaccagt tcacgcagat gttccagggt
    21601 cacaatccgt tcaaccggat atttacttac cgccagacga cgcaggtaaa taacatgcgc
    21661 gccacaaccg agtttttcac ccaggtcatc aatgatggtg cggatataag tgccttttga
    21721 gcagtgaatt tccagctcca gctcattgcc ttcgtggcga ataaacagca attcataaac
    21781 ggtaatcggg cgcgcttcac gcggtacttc aatgccctga cgcgcatatt cgtacagttt
    21841 tttaccctga tatttcagtg ctgaatacat cgaagggatc tgttcgatat cgccacggaa
    21901 agtatccagc gccgccgcca gttgctctgc gctaaaggtt accggacgtt cttcaacgat
    21961 ttgtccgtcg gcatcagaag tatcggtacg ctgtccaaga cgcgcaatga cccgatagcg
    22021 tttgtcggag tccagcagat actgggaaaa cttcgtcgct tccccgaggc aaattggcaa
    22081 catgccagtt gccagcgggt ccagcgcacc ggtatgcccg gcacggttgg cgttataaat
    22141 ccgtttcact ttttgcagcg catcattgct ggacataccc tgcggtttat ccagcaacaa
    22201 aacgccgtta atgtcgcgac cgcgacgacg aggacgactc attagtcctc cttgctgtcg
    22261 tccgggttaa cacgacgttc ttcgtcatgt ttgaccacgc tggtcaccag gtttgacatg
    22321 cgcatccctt caaccagaga gttgtcgtag aagaaggtca gttccggcac gatacgcagg
    22381 cgcatcgctt tccccagcag gctgcggatg aaaccagaag cttcttgcaa cgctttgatg
    22441 cccgctttaa ccgcgtcttc atctttgtcg ttgaggaacg tcacatatac tttggcatac
    22501 gccaggtcgc gagacatttc gacaccggaa acggtggtca tcatgcccag gcgaggatct
    22561 ttaatttcac gctgcaggat gagagcgatc tctttttgca tttcctgcgc tacgcgctgc
    22621 gggcgaccaa attctttcgc cataataaat tctcctgaca aaaaaggggc tgttagcccc
    22681 tttttaaaat taatttcagg tggaagggct gttcacgttg gcctgataag acgcgccagc
    22741 gtcacatcag gcaatccatg ccggatgcgg cgtaaacgcc ttatcccgca tggaacccta
    22801 aaaaccttaa gcgatggtac gttggatctc gatgatttcg aatacttcga tcacatcgcc
    22861 agtgcggacg tcgttgtagt tcttaacgcc gataccacat tccataccgt tacggacttc
    22921 gttaacgtca tctttgaagc ggcgcaggga ctccagctcg ccttcgtaga taaccacgtt
    22981 gtcacgcaga acgcggatcg ggttgtgacg tttaaccaca ccttcggtaa ccatacagcc
    23041 tgcgatggca ccaaatttcg gcgatttgaa cacgtcacga acttccgcca gaccgataat
    23101 ctgctgtttc agttccggag acagcatacc gctcatcgcc gctttcactt cgtcaatcag
    23161 gttatagatg acggagtagt aacgcagatc caggctttcc gcttcaatca ctttacgtgc
    23221 agaggcatca gcacgtacgt tgaagccaac caggatggcg ttggacgccg cagccagggt
    23281 ggcgtcggtt tcggtgatac cacctacgcc agaaccgatg atcttcactt taacttcgtc
    23341 agtagacagt ttcagcaagg agtcggagat cgcttccaca gaaccctgta cgtctgcctt
    23401 caggacgatg ttcacttcgt gaacttcgcc ttcggtcatg ttggcgaaca tgttctcgag
    23461 tttagatttc tgctgacgcg ccagtttaac ttcgcggaat ttaccctgac gatacagtgc
    23521 aacttcacgc gctttcttct cgtcacgtac aacggtaact tcatcacccg cagccggtac
    23581 gccggacagg ccgaggattt ccaccggaat ggacggaccc gcttccagca cttcctgacc
    23641 cagttcgtta cgcatcgcac gaacacgacc gtattcgaag ccacacagaa cgatatcgcc
    23701 cttgtgcaga gtaccttcac gtaccagaac ggtagcaacc ggaccacgac ctttatcgag
    23761 gaaggattcg ataaccgcac cgctcgccat acctttacgt accgctttca gctccagaac
    23821 ttccgcctgc agcaggatag cgtccagcag ttcatcgata ccagtacccg ctttcgcaga
    23881 cacatgtacg aactggcttt caccgcccca ctcttccggc aggatgccgt actgggagag
    23941 ttcgttctta acgcgatccg gatcggcttc tggtttatcg atcttgttca ctgcaacgac
    24001 caccggaacc tgcgccgctt tcgcgtgctg gattgcttcg atcgtctgcg gcattacgcc
    24061 gtcgtcggca gcaacaacca ggactacgat gtccgttgcc tgcgcaccac gagcacgcat
    24121 tgaagtaaac gcggcgtgcc ccggggtgtc caggaaggtg atcatgccgt tttcagtttc
    24181 aacgtggtat gcaccaatgt gctgggtaat gccgcccgct tcgccagagg ccactttcgt
    24241 tgaacgaatg tagtccagca gagaggtttt accgtggtca acgtgaccca tgatggtcac
    24301 aaccggcgcg cgcggttcag ccgcagcacc cgtgtcacgg tcgctcatta ccgcctcttc
    24361 cagctcgttt tcacgacgca ggataacttt atggcccatc tcttcagcaa ccagctgtgc
    24421 ggtttcctga tcgataacct ggttgatggt tgccattgcg ctcagtttca tcatcgcttt
    24481 gatgacctga gagcctttaa ccgccatctt gttcgccagt tcgccaacgg tgatagtttc
    24541 gccgatcaca acgtcacggt taacggcctg agcaggcttc tggaagcctt gctgcagcga
    24601 agaaccttta cgttttccgc ctttaccgcc acgtactgct gcgcgtgctt cttcacgatc
    24661 agcttttgat tcagcgtgtt tgttgccttt cttcggacgc gctgctttcg cgttacgacc
    24721 acggccacgg ccgccttcga cttcacgatc gctttcgtct tctgcctggc gagcatgttg
    24781 agaagtagtg acgtgataat cgctggaatc ttcagtcggt tccgcgttat cagtccattt
    24841 gttttcttcc gccatacgac gtgcttcttc agcaacgcga cgtgcttctt cttcgagttt
    24901 acgacgcgct tcttcttcag ctttacgctt gagctctgca gcttcctgct cacggcgggc
    24961 tttttcagcc tgggcgtttt tagtcatatc gtcttgttga ttgctcactt tgtctttttc
    25021 cgcagcttca cgtttcgctt gttcagcagc ttcacgctta gcttgttctg cggcctcacg
    25081 ttcagctttt tgttgcgcct cgcgtttagc cgattcttct gcctcacgac gggcttgctc
    25141 ttccgctgca aggcgttcag cctcttgcgg atcgcgtttc acaaaggtgc gtttcttgcg
    25201 gacttcgatt tgtaccgatt tgctttttcc accggtacca ggaatgttaa gggtgctgcg
    25261 tgttttacgt tgcagcgtca atttgtccgg gcctgaattt ttctgattca ggtggtcaat
    25321 caaagtctgt ttctcttgtg cagacacaga gtcgtcagca gacttccgga tacctgcatc
    25381 agcaaattgc tgtaccaggc gttccacgga ggtctgtcgc tctgcggcca gcgttttaat
    25441 cgttacatct gtcatgctgt tccttcctgc tacagtttat tacgcttcgt caccgaacca
    25501 gcaaatatta cgggcagcca taatcagtgc tccggctttt tcgtcggtca acccttcgat
    25561 atcagccaga tcatcaatgc cctgttcggc gagatcttcc agcgtacaaa cgccacgggc
    25621 ggccagtttg aatgccaaat cacgatctac cccttcaagg ttcagcagat cgtcagccgg
    25681 tttgttatca ccgaggcttt cttcctgggc ctgtgcaatg gtggccagtg catttttagc
    25741 acgctcgcgc agtgcttcaa cggtcggctc atcaaggcct tcgatttcca acagctcttt
    25801 catcggcaca taggccagtt cttccagcgt cgagaaacct tcttctacca gaacagtcgc
    25861 gaagtcttcg tcgatgtcga gatatttggt gaaggtgtcg atcgctgcgt gcgcttccgc
    25921 ctgatgctta gcctgcaggt cgtcaacggt cattacgttg agttcccaac cgctcagctg
    25981 cgaagccaga cgcacgttct gaccgttacg gccaatcgcc tgcgccaggt taccagcttc
    26041 aacggcgata tccatggtgt gtttatcttc atccaccacg atagaagcaa cgtctgccgg
    26101 tgccattgcg ttaatcacaa actgcgccgg gttatcatcc cacaggacga tatcgatacg
    26161 ctcgccgccc agttcagtag aaaccgcctg aacacgcgcg ccacgcatac ctacgcaagc
    26221 acctaccgga tcgatacgtt tatcgttggt tttcaccgcg attttcgcac gagaacccgg
    26281 gtcgcgagcc gctgctttaa tttcaatcac ttcttcgccg atttccggca cttcaatacg
    26341 gaacagttcg atcagcattt ccggcttgga acgagtgacg aacagttgcg cgccacgcgc
    26401 ttccgggcga acggaataaa gcacgccacg aacgcggtcg ccagggcgga agttttcacg
    26461 cggcagcata tcttcgcgca ggatcacggc ttcagcgttg ttgcccagat ccagagagat
    26521 gttgtcgcgg tttacttttt tcaccacgcc ggtgatgatt tcaccttcgt gttcacggaa
    26581 ctgatcaacc accatcgcac gttcggcttc acgcactttc tgcacgataa cctgttttgc
    26641 cgtctgggtg gtgatacggt caaaggtaac agactcaatc tgatcttcaa cgtaatcgcc
    26701 caagttcagg ctttcatctt cataacgtgc ggcttcaagg gtgatttcct tggtcggctg
    26761 ggtgacttca tcaacaacta accagcgacg gaaggtgtca aaatcaccgc ttttgcgatc
    26821 gatctgtaca cggacgtcga tctcttgctc atattttttc tttgttgctg tcgccagcgc
    26881 gctttccaat gcttcgaaaa tcttctcgcg aggtagcgcc ttttcattgg atacggcttc
    26941 aactacagcc aaaatttctt tgttcatcgc gggcttttca cctcatccag actattaaaa
    27001 gtggggaacc aggttcgcct tctggatatt actcagcgcg aacacttcat ctttaccttc
    27061 gacggtaact gtgatcattt caccgtctac cgctttgata acgccctgcc atttacgacg
    27121 gttttgtacc gccatacgga gaaccagagt cacctcttct ccgacaaaac gggcgtagtg
    27181 ttcagccgtg aacagtgggc gatcgagacc cggtgaggag acttccaggt tataagcaac
    27241 ggtgatggga tcttcaacat ccagcacagc acttacctgg tggctcacat cagcacaatc
    27301 atcaacattg atgccatctt cactatcaat atagatgcgc agtgtggatg tgcgaccgcg
    27361 aataaattcg atgcctacca gttcaaaacc cagggcctca actggcgcag taatcatctc
    27421 tgttaatttt tgctctaatg tggacaagcc cacccccaag acataaaaaa agggcctaaa
    27481 gcccagttat tctgtagtca gataacaaaa aaccccgata aatcggggct ttatataact
    27541 gaaccctata accgcaactg cggtctggag cactttccag aaggattttt tcaaatccca
    27601 ctacgaaggc cgaagtcttc acagtatatt tgaaaaagga ctctaaggga aagtggttgc
    27661 gggggccgga tttgaaccga cgaccttcgg gttatgagcc cgacgagcta ccaagctgct
    27721 ccaccccgcg cctgaaacgt ggcaaattct actcgttttg ggtaaaaaat gcaaatactg
    27781 ctgggatttg gtgtaccgag acgggacgta aaatctgcag gcattatagt gatccatgcc
    27841 acattttgtc aacgtttatt gctaatcacg tgaatgaata tccagttcac tttcatttgt
    27901 tgaatacttt tgccttcccc tgctctccct taagcgcatt attttacaaa aaacacacta
    27961 aactcttcct gtctccgata aaagatgatt aaatgaaaac tcatttattt tgcataaaaa
    28021 ttcagttaga gcggaaatcc aggctcatca tcagttaatt aagcagggtg ttattttatg
    28081 acgacgattc tcaagcatct cccggtaggt caacgtattg gtatcgcttt ttccggcggt
    28141 ctggacacca gtgccgcact gctgtggatg cgacaaaagg gagcggttcc ttatgcatat
    28201 actgcaaacc tgggccagcc agacgaagag gattatgatg cgatccctcg tcgtgccatg
    28261 gaatacggcg cggagaacgc acgtctgatc gactgccgca aacaactggt ggccgaaggt
    28321 attgccgcta ttcagtgtgg cgcatttcat aacaccaccg gcggcctgac ctatttcaac
    28381 acgacgccgc tgggccgcgc cgtgactggt accatgctgg ttgctgcgat gaaagaagat
    28441 ggcgtgaata tctggggtga cggtagcacc tataaaggaa acgatatcga acgtttctat
    28501 cgttatggtc tgctgaccaa tgctgaactg cagatttaca aaccgtggct tgatactgac
    28561 tttattgatg aactgggcgg ccgtcatgag atgtctgaat ttatgattgc ctgcggtttc
    28621 gactacaaaa tgtctgtcga aaaagcctac tccacagact ccaacatgct tggtgcaacg
    28681 catgaagcga aggatctgga atacctcaac tccagcgtca aaatcgtcaa cccgattatg
    28741 ggcgtgaaat tctgggatga gagcgtgaag atcccggcag aagaagtcac agtacgcttt
    28801 gagcaaggtc atccggtggc gctgaacggt aaaaccttta gcgacgacgt agaaatgatg
    28861 ctggaagcta accgcatcgg cggtcgtcac ggcctgggca tgagcgacca gattgagaac
    28921 cgtatcatcg aagcgaaaag ccgtggtatt tacgaagctc cggggatggc actgctgcac
    28981 attgcgtatg aacgtctgtt gaccggtatt cacaacgaag acaccattga gcagtatcac
    29041 gcgcatggtc gtcagttggg ccgtctgctg taccaggggc gttggtttga ctcccaggcg
    29101 ctgatgctgc gtgactctct gcaacgctgg gttgccagcc agatcactgg tgaagttacc
    29161 ctggagctgc gccgtgggaa cgattattca atcctgaata ccgtctcaga gaacctgacc
    29221 tacaagccag agcgtctgac gatggaaaaa ggcgactcgg tgttctcgcc agatgatcgt
    29281 attggtcaat tgaccatgcg taatctggat atcactgata cccgagagaa actttttggt
    29341 tatgccaaaa ctggcctgct ttcctcctct gccacttcag gcctgccgca ggtggagaat
    29401 atggaaaaca aaggccagta attcgacatc aaccctgccc ttcaggggtg gggttacctt
    29461 gcttatcctc tgtaaactcc tttctgagca tcacataatc gaaaacatca tggtttgcat
    29521 ccacaacccg tgagtctcct ttatagtgtg caatcacttc ctcaagcgtt tgcggctgtt
    29581 ccggctttgt tacccccatc cacgcattaa taaggtaatt attgtaagct gtcgaaaaga
    29641 tattgttttc cgttgtgcga tccacgccat ccccaagaac ggggctatac cagataaaca
    29701 tcgggacatg atatgcctgc tggctagcct ccctgcctcc atgaaaatag acgttcttct
    29761 tcgtagggtc acgttccagg ccgtggtcgg caaaatacat gaccgaggcg cggcgatctt
    29821 ttaataattc aaaaacctga cctagcaaac tatctgtgta atgaatggag ttgtcatagc
    29881 aggcatcctg atcgtcctgc ggttgaaaca cggcgctgga ttgcggatag gcgctacaag
    29941 ccggttcatg gcttccattt aaatgaagaa caatcagttt cttttgctgc gtattttgct
    30001 gtaatgcttg cgataaatgc ggcaacaaca attcatcaaa tcctctgaca taaactgttt
    30061 ccatggcgcg catggcgata ctggtaactg ctgtaccatt ctgccgaaaa gcggattgcg
    30121 agcttagcca gaaagtctga aatcctgctt gattagccat attaataatg ttgtcggggt
    30181 aattatgaat gtcatgactc aaaacagaat cagcagttaa agaaagggga accgacagcg
    30241 cagtgtaagg tgcgccgctt attgcttgat taaacagttt gatctgtttt ctttgtgctt
    30301 caacttgcgg tgtcgtagag cgtgtatatc catacaaaga catattgtcg acacgtacag
    30361 actcccccac aatcaacacg taggtatcaa tacttgtatc cctgactgat aattgaaaat
    30421 acggcaccgt gtttgcaatt gagagtaatc tttgatgctc tttcgctgct aaagcaaaat
    30481 agttgagatt gaaaaacggc gtataggtag caaatcacga cgctagtata tatggactga
    30541 acgctttttt attttttgca tctttataag caaattgaca agcggaaaat aaactgcccg
    30601 aaatgacaat cagcaataat attcctgtca ctttttttgt cggcaaggaa acatcatatt
    30661 ttattattac tgccaaaaaa agaagggata aaaaagcaaa agcacataga taagggatat
    30721 acatccccag cattttcaca acttcatccg gatcactctg cagcacacta atcgcaaatc
    30781 catcattgaa agttgtacca aaggtacacc atgaatagag gctaatactc atgtctgccg
    30841 cagtcagtaa cacaaatggt gcggcaacaa gcaggcgaag cggaagggat gatatacgtt
    30901 taacaaccag gatggtcagg acgaagaaca gcaatcgctg aaccatatgc gctccggaag
    30961 aggcgactaa gttcgtaata ccaaaaacaa taacacaaag atacaacaac caatgagatt
    31021 taaaatcttc tagcaaattt gttgaatact gtcatccaga aatccaagtt cacaaccaat
    31081 ataaacaatt atcaacaatg ttcaatttta tatcagacat gtttttcaac tctgatccat
    31141 atcagaccga aacactgtta gttttttctt tttaaatgat gcgaccattg cgttctcagt
    31201 aattaaataa cattatcaat gcgtctctaa acattacgca gaacgtagcc atcagtagtt
    31261 atactgacag gagcaggatg gtttatcgag aagtaaacat atccgtattc gtaccggaac
    31321 tggagttgac acaataaagt gccaattatg tcagcagtag ggaaaagttt ataactaagg
    31381 cgtaccggca ccatcgtttc aaggtaccag ctacgagtaa agcaactgga cgagatacag
    31441 atacctgaca accattcatc agaccaggac caaaacgaaa agagacgctt ttcagcgtct
    31501 cttttctgga atttggtacc gaggacggga cttgaacccg taagccctat tgggcactac
    31561 cacctcaagg tagcgtgtct accaattcca ccacctcggc acggatacta ctttttagtt
    31621 cgggatatcg ctggtcggct tagccggagc agctggctga gtttgttcgg ttttcgccgg
    31681 tgcactcaga ttttcccatt cgctacctct attggttttg ttgctattga tgttacccag
    31741 caccagactg atgatgaaga ataacgttgc cagcagcgcc gtcatgcggg tcatgaagtt
    31801 accagaacca cttgaaccaa acagcgtagc ggaagcgcct gctccgaagg aggctcccat
    31861 atcagcgcct ttaccttgct gcagcatgat cagaccaaca aggccaattg ccacaataag
    31921 gaaaactact aaaagagctt cgtacataat caacctgttc cttgcggagt tgccgcgtac
    31981 caatgcttca accaataaag cgggagtttt taatctttcc cactgaagcg ggtgtgaata
    32041 ctaaccaaaa cgaatgacct tcgcaagggc aattttatcg cattgtatca actgcggaaa
    32101 aaaacagcaa aaccgatgtg ttcgccgaga aaaaaggcga caatcgccgc ctttttagcc
    32161 agttatctaa cgctttaaac ggcttttact gcatcggcga tgcggtgtgc aaattcagtc
    32221 acctgcgctt cgtcttcgcc ttccaccatc acgcgaatta acggttcggt gccggattta
    32281 cgcagcaaca cgcgtccacg gttgcccagc gcagcttcaa cctctgcggt cacggcttta
    32341 actgactcat gctcaagcgg atcgccgcta cctgcggtgt aacgtacgtt aaccagaatc
    32401 tgcgggaaca ttttcatgcc gctgcaaagg tcgtgcaggc tcatatggtt acgtgccatc
    32461 gccgccagca cctgcaagcc agcaacgatg ccgtcaccgg tagtggtttt atccagcagg
    32521 atcacatgac cggaattctc tgcaccgata cgccagcctt tctcctgcat tttttccagt
    32581 acgtagcggt cacccacttt cgcgcgcgca aatggaattc ctaactgttt cagcgccagt
    32641 tcaagcccca tgttgctcat caatgtaccc acagcaccac cacgcagctg gccctgacga
    32701 agaccttcac gcgcgatgat atacatgatc tgatcgccat cgactttatt gccttcatgg
    32761 tcaaccataa tcacgcgatc gccgtcgccg tcgaaggcaa taccgagatc cgctttttca
    32821 gccagcacac gagcctggag cgcgcgaacg tcggtagccc ccacttcggc attgatgttt
    32881 acaccgtttg gctcacaacc gatagcgata acgttcgccc ccagttcgcg cagcacgttc
    32941 ggcgcgatgt gataagtcgc accgtttgca caatccacca caatcttcag ttcactgagg
    33001 ctaagttcgt tcgggaacgt ggctttgcaa aactcgatat agcgacccgc ggcatcaacg
    33061 atacggctgg ctttacccag ttctgccgaa tcaacgcagc tgatctcctt ttccatttcc
    33121 gcttcgatgg cctcttctac cgcatccggc agtttagtgc cgtcgataga gaagaattta
    33181 atcccattat cgtagaacgg gttatgcgat gcagatatca caattccggc ctctgcgcgg
    33241 aaggtacgcg tcagataagc cacggccggt gttggcatcg ggccagtgaa gagtgcggaa
    33301 aggcccgctg ccgccagacc cgcttccagt gctgactcca gcatatagcc agaaatacgc
    33361 gtgtctttac caataataat cttacgggag ccgtggcgcg ccagcacttt acccgcggcc
    33421 caacccagct taagcacaaa atcaggtgtg atcggcgcat cccctacacg accacgaatc
    33481 ccatcggtac cgaaatattt acgattactc atagcgtttg ttttcctttg cagacagagt
    33541 ggcttccacc acccgcatcg cttctacggt ttctttgacg tcatgaacac gaatgatgtg
    33601 cgcgccttgc attgcggcaa tgaccgcaca ggccagacta ccgctcaggc gctcggacgg
    33661 ccccacgttc agcagctgcc caatcatcga ttttcgtgac atacccacca acagcggcag
    33721 gttgaaatgg tgaaattcag ccaggcgcgc cagtaatgaa tagttatggg agagattttt
    33781 accgaaaccg aatccggggt cgagcaacaa tttctctttt gcgatacccg cctgctcgca
    33841 acgtgctatt tgctcaataa agtagcgatt cacttctgca aagacatcgt catacttcgg
    33901 agcttcctgc atggtttttg gatttccctg catatgcatc agacaaaccg gtaaaccggt
    33961 ttctgcagcc gcctccagag cgccaggttc ggaaagggag cggatatcat taataatgtg
    34021 agcgccaact ttcgctgact cacggatgac ttctggtttg gatgtatcga ccgagatcca
    34081 gacttcgaag cgttgagcaa ttgcctcaac cacaggaata acacgttgca actcttcttc
    34141 aacgctaact tccgccgccc ctgggcgcgt ggactcgcca ccaacgtcaa tgatcgtcgc
    34201 gccagcattg atcatcagat tcgcatgttt caccgcatct atcagcgagt tatgcgtgcc
    34261 accatccgaa aaggaatcag gcgtgacgtt gaggatcccc attacgtgag gatggctaag
    34321 gtccagtgaa gtaccctggg caaagagttt cattatgtta tccctggtat gaattgataa
    34381 gaaaaaaccc cggagcacgc cccggggttt tcggtacaaa tacagtcatc tgatgcggga
    34441 acttacttgt cgcctaactg ctctgacatg gtgttacccg ggttcggcgt acgcggttca
    34501 tcaaccggac gaggagcctt tggactacca ttgtcgccag aattgttaga agcgcctggt
    34561 tcttcccagc ccgctggcgg acgtacatcg cgacgtgcca tcaggtcatc aatctgcggt
    34621 gcgtcgatag tctcatattt catgagagca tctttcatcg catgcagaat atccatattg
    34681 tcggtcagaa gctgacgcgc acgattatag ttacgctcaa tcagtgcttt cacttcctgg
    34741 tcgatgatac gtgcagtttc atcggacata tgtttcgctt tcgctacgct acggccgagg
    34801 aatacttcac cttcttcttc cgcgtacagc agtggaccca atttctcaga gaagccccac
    34861 tgagtcacca tgttacgtgc caggttggtc gcaactttaa tatcgttgga cgcaccggta
    34921 gaaacatgtt ccggcccgta gatgatctct tctgccagac gaccaccgta cagcgtagaa
    34981 atctggcttt ccagtttctg acggctggcg ctgattgcgt cgccctcagg caagaagaaa
    35041 gtcacaccca gcgcacgacc gcgtgggata atcgtcactt tatgcaccgg atcgtgttcc
    35101 ggcaccaggc gaccgataat cgcatgaccc gcttcgtggt aagccgttga ttctttctgc
    35161 gcttccgtca tcaccatgga gcgacgttcc gcacccatca tgattttgtc tttcgctttc
    35221 tcgaactcaa ccatcgacac aacgcgtttg ttgccacgag cagcgaacag tgccgcttcg
    35281 ttcaccaggt tcgccaggtc agcaccggag aaaccaggag taccacgggc aatgattgcc
    35341 gcgtcgatat cgggtgccaa tggtacgcga cgcatgtgaa ctttcaggat ctgctcacga
    35401 ccgcgaacat ctggcaagcc gaccacaacc tggcggtcga aacggccagg acgcagcagg
    35461 gccgggtcga gaacgtccgg acggttagtc gcggcgataa cgatgatacc ttcgttacct
    35521 tcgaagccat ccatctcaac cagcatctgg ttcagagtct gttcacgttc atcgtgacca
    35581 ccgcccagac cagcgccacg ctggcggcct acggcgtcga tttcatcgat aaagatgatg
    35641 cacggtgccg ctttcttcgc ctgttcgaac atgtcacgaa cacgggatgc acccacaccg
    35701 acgaacattt ctacgaagtc agaaccggag atagtaaaga acggaacttt cgcttcgcct
    35761 gcaatcgctt tcgccagcaa cgttttaccg gtacccggag gaccgaccat caggacgcct
    35821 ttcgggatct taccgccgag tttctggaag cggctcggct cgcgcagata ctctaccagt
    35881 tcagcaactt cttcttttgc ttcgtcgcag cccgcaacgt cagcaaaggt cgttttgatc
    35941 tgatcttccg tcagcatgcg cgctttgctc ttaccaaacg acatggcacc tttgccaccg
    36001 ccgccctgca tttgacgcat gaagaagatc cagacaccaa tcagcaacag catcgggaac
    36061 caggagatga agatagaagc cagcaggctt ggttcttcag gcggttcacc gacaaccttg
    36121 acgttcttgg tcaacaggtt atccagtaat ttcggatcct gaaccggaat gtaagtggta
    36181 taacggttac tatctttctt ggtaacgttg atttcacgtc cgttgatacg cgcttcacga
    36241 acctggtcgt tattgacctc ttgtaggaag gtagagtaat ccaccttacg gccattagac
    36301 tcgctgggcc caaagctctg gaatactgac atcagcacaa cggcaatgac cagccagagt
    36361 attaggtttt tcgccatgtc actcaaggga ttaacctctt attacaactg tgttaaaaac
    36421 agcgtcagga tactctatat ccagcatctt tcaaactttc gtctgaaatc tcccggttag
    36481 ggtttacgcc cggtcgctac aatatacact tctcgcgaac gtgcacgaga agagtccggc
    36541 ttacgaactt tgaccttcgt aaacagggag cgaatttccc ttagatactc atcgaaacct
    36601 tcgccctgga acaccttcac tacaaaactg ccacctggcg ctaatacatc acgacacatt
    36661 tctagcgcca gttccaccag atacatggct cgggggatat ccaccgccgg tgttccgctc
    36721 atgtttggtg ccatatcgga catgacaacc tggactttgc tgtcgccaac gcgctccagc
    36781 agtgctttca tcaccagttc atcacgaaaa tcgccctgaa gaaagtccac accaacgata
    36841 ggatccatag gtaaaagatc gcaagcgatg atgcggcctt tgccgccaat ttgggtgacc
    36901 acatattgtg accaaccacc cggagcagca ccaaggtcga caaccgtcat tcccggttta
    36961 aagagtttgt cactttgctg tatttcatca agtttaaacc aggcacggga acgtaatccc
    37021 tttttctgtg cctgttgaac atatttatcg ctaaagtgtt cctgaagcca gcggctggag
    37081 ctggcagaac gcttcttacc tgtcatttaa ctttcccatg gggataactc atcgtaacca
    37141 attgcgtaaa tttttacgcg cctatttggt gatatatggg agatggcggt agaatgaccc
    37201 gttttcaatc ccaacgtaag caaaaaatac gatgaatctg agtactaaac aaaaacagca
    37261 cctgaaaggt ctggcacatc cgctcaagcc agttgttctg cttggcagta atggtttgac
    37321 cgaaggggtg ctggccgaga ttgaacaagc gttagagcac catgaactca tcaaggtgaa
    37381 aatcgccacc gaagatcgcg aaacaaaaac cttgatcgtg gaagctatcg tgcgcgaaac
    37441 cggcgcctgt aatgtacagg tcatcggtaa aacgctggtg ctttatcgcc caactaaaga
    37501 acgtaaaatc tcgctgccac gctaagatta tcctaaagtt acacacattt gctgtgtaaa
    37561 acgaggggtt ttccgcaggc aggagagcaa aatgccacgc tctgttcgtt gataaaaggc
    37621 cgcagagcgg cctttttcct ttctttacaa tacatcaaca tcttgagtat tgggtaattc
    37681 ttacaggtat tccaccttaa ttacttcaaa ttctacttcg ccgcccggcg ttttgatgac
    37741 cacaacatca tcttcttctt tgccgatcag gccacgagca ataggcgagt ttacagaaat
    37801 caggttttgt ttaaagtcag cttcgtcatc gccaacgatg cgataagtct gttcttcgtc
    37861 agaatccaga ttcagcaccg ttacggtagc accaaaaata acgcgcccat tgttgggcat
    37921 tttggtgaca tcaatcacct gcgcgtttga cagcttggct tcgatgtctt tgatacggcc
    37981 ttcgcagaag ccctgctgtt cacgagctgc gtggtattcg gcgttttctt tcaggtcgcc
    38041 atgctcacgc gcttccgcga tagcagcaat gatttcagga cggcgcacag atttcagaaa
    38101 atccagctct tcgcgtaatt tttcagcgcc gcgtaaggtc atcggaatag cttgcatttg
    38161 ttatacctct tgaatattcc tgatggggca agtcttcacc caccccggct gttcggcctg
    38221 cccggcataa ctcctcaccg ggaccagaag caaaaaaata ccgacccggg tacaagtccc
    38281 aggtcagcta caattcacat tttgatggtc attttaccct gaagttcccg aagggtcatc
    38341 gtttacttta tagggcgttg cgccgtagta tgacggctcg attccaggtt gttagcgcga
    38401 gattatgcga ttttccagat ttatcatcgg attgaccagc tgtatagcgt tcagtgttca
    38461 ggccgcgaat gttgatgagt acgttactca actccccgca ggtgccaacc ttgccctgat
    38521 ggtgcaaaaa gtcggtgcgt cggcccccgc tattgattac cacagtcagc agatggcgct
    38581 gcccgccagt acccagaaag tgattactgc gctggcggcg ttgattcaac tcggccccga
    38641 ttttcgtttc accacgacgc ttgaaaccaa aggcaatgtg gaaaacggcg tacttaaggg
    38701 tgacttagtg gcgcgatttg gtgccgatcc gacgttaaaa cgtcaggata ttcgcaatat
    38761 ggtcgcgact ttgaaaaaat ctggcgtcaa ccaaatcgat ggcaatgtgt tgatagatac
    38821 ctccattttc gccagccacg ataaagcccc cggctggcca tggaatgaca tgacacaatg
    38881 ctttagcgct ccgcctgctg ccgccatagt tgaccgcaac tgtttctccg tctcgctcta
    38941 cagtgcccca aagcctggtg atatggcttt tatacgcgtg gcatcttatt accccgttac
    39001 gatgttcagc caggtacgca ccctcccccg tggttctgcc gaagcgcaat actgcgaact
    39061 ggatgtggtg ccaagcgacc tgaaccgctt tacgctgacg ggatgcctgc cacaacgttc
    39121 tgagccgctc ccgttggctt ttgccgtgca ggatggagcc agctatgccg gtgcaattct
    39181 gaaagatgag ttaaaacagg cgggtatcac ctggagcgga acactgctgc gccagactca
    39241 ggttaacgaa cctggaacgg tagttgccag taaacagtcg gccccgctgc acgatctgct
    39301 taagattatg ctgaaaaagt cggacaacat gatcgccgat acggttttcc gcatgatcgg
    39361 ccatgcgcgc ttcaatgtgc ctggaacatg gcgggctggg tcggacgccg tgcgtcagat
    39421 cctgcgccag caagccggtg tcgatattgg taacaccatt attgccgatg gttcagggct
    39481 ttcgcggcat aacctgattg cccccgccac catgatgcag gtgctgcaat acattgccca
    39541 acacgacaat gaacttaact ttatctccat gctgccgctt gcgggctatg acggctcttt
    39601 gcagtaccgt gcaggtctgc atcaggcggg cgtggatggc aaagtctcag cgaaaaccgg
    39661 ttcgttgcag ggggtatata acctggcggg attcattacc acagcgagcg ggcaacgaat
    39721 ggcatttgta caatatcttt ctggctatgc agtaaaaccc gcggatcagc gtaatcgccg
    39781 tattccgtta gtgcgttttg aaagccgttt gtacaaagat atttatcaga acaattagtc
    39841 aaaaagaaac cccggcgcat ggccggggtt tcagattatt gacaaagtgc gctttgttta
    39901 tgccggatgc ggcgtgaacg ccttatccgg cctacaaaat cgtgcaaatt caatatattg
    39961 caatcatctt gtaggcctga taagtgtagc gcatcaggct tatttggcat tggtcattag
    40021 tgattaacgc ttgtaaatga actcaacgcc ttcttcgtcg tcttcgtccc agtcgtcatc
    40081 ccagtcttca tcatcctctt cagcaatctc ttcaagctgc tgacggtgat aatcatccca
    40141 catgaattcg actttctctg gctgtttcgc ttcttcagcc tgcacgaccg ggttttcaat
    40201 gataaaggtc atcacatccc agcagagatc tttcacgccc agtccgctcg ccgcagagat
    40261 cagataatat ttatcttccc agcccagcgc ttcagcgatc actttcgctt tctcttcggc
    40321 ttctgcctta tccagcagat cgatcttgtt gaagactaac caacgcggtt tcgccgccag
    40381 atcctggctg tatttttcca gctcgctgat aataatacgc gcgttttcaa ccggatcggt
    40441 gccgtcaatc ggatcgatat cgatgaggtg caacaggacg cggcaacgtt ccaggtgctt
    40501 caggaagcga atgcccagac ctgcgccttc cgcagcgcct tcaatcagtc ctggaatatc
    40561 ggcaacaacg aagctctttt cgttgtccat tcgtaccaca cccagacttg gcaccagagt
    40621 ggtaaacgga taatccgcca ctttcggttt agccgccgat accgcacgaa taaaggtcga
    40681 tttacccgcg tttggcatcc ccaacatacc gacgtcagcc agcagcatca gctccagcag
    40741 cagctcacgc ttatcgcccg gcgtaccgtt agttttctgc cgtggtgtac ggttaactga
    40801 cgatttgaaa cgggtattgc ccagaccgtg ccagccgccc ttagcaacca gcagacgctg
    40861 accgtgttta gtcatatcac ccatggtttc accagtaccc tggtcgatta cacgcgtacc
    40921 taccggcact ttaatcgtca cgtctttacc gcgcttaccg gtacaatcgc ggcttgcgcc
    40981 gttctgaccg cgctctgcac ggaaagattt ttcaaaacga taatcgataa gcgtgttcag
    41041 gttctcgtcg gcttccatcc atacgtcacc accatcaccg ccgtcgccgc catccgggcc
    41101 gcctttcgga atatactttt cgcggcggaa gctcacgcaa ccattaccgc catcacctgc
    41161 aacgaccaga atcgatgctt catcaacaaa cttcatttta ttctccgtaa atcattcgcc
    41221 tgcgcggggt tgcgatacca ccgttgtatg cttacgtaat ccgccccaaa tacgatgacc
    41281 aatggcggaa tacatcgcgc ccgcaaccac gacaaacgca ccgagataac ctaaaaggtt
    41341 taacatcggt ctggcgaaga aatcgggcca ggccagtgat aaaagatctg aaaaaaacag
    41401 cgtaaacagt ggggtgagcg tgatgatcgc gctcacctgc gctgcctgcc agcgagccat
    41461 cgcttccgcc aaggcgccat atcctaccaa ggtattcagt ccgcaaaaaa ttaaacatgc
    41521 gagctgccag tggctaagct gcgctatcac tccaggcttt gccagaggga agagcgcgat
    41581 tgtacataaa gtgtacagta aaaacaggat ctgcggtgag gccagccgac gcaataaaac
    41641 cttttgcgcc acgccataac tcacccaaac cgtcgccgca ccgaccccaa agataactcc
    41701 ccaggtgtaa tcggtgagct ttgtaaatat ctcgaccaga ctggtgttaa aaaacatcac
    41761 cagaccgctc aggagcatca atgcccctac aacctgagtg ctgcgcattt tctctttcag
    41821 gataaatacg ctggcaacca tcatgccaac tggcgagagt tgcccaatca cctgcgaagc
    41881 ggtcggactc aggtattgca aggatgagct gaacaggatg aagttcccaa acagcccggc
    41941 ggtcgccact gccaacaaaa tcaaccagcg tggcttacga aacacgcgta atggcggcaa
    42001 cctcttcttc accgcaagaa tggcaccaag gccaatactc gccatcaaga aacggtaaaa
    42061 cacgattgtc ggaggttcca tcacctccag cacctgcttc attgcgattg gcaacgcccc
    42121 ccagcaaatt gctgtggtaa gcgccaaaag aatgccaatg cctgcctgct gcttcatacc
    42181 gtattccctg caaagcgcat ttgcggatta acgtcgcacc atgcgcattt ttaccggtta
    42241 tcgaatgtaa aaagccccgc aacgtgttgc ggggctttca tccgttaccg ggacgcgaaa
    42301 aacttattca gcttcgatgc tgataaattt acggtttttc gggcctttaa cttcgaattt
    42361 cactttaccg tctgctttag caaacagagt gtggtcacga ccgcaaccta cgttagcgcc
    42421 agcgtggaat ttggtaccac gttgacgaac gatgatgcta cccgccagaa cggattcgcc
    42481 accgaaacgc ttaacgccca agcgtttagc ttctgaatcg cgaccgttac gtgtggagcc
    42541 gccagccttt ttatgtgcca tttgaaatct ctcctcaggt cttaggcgct gatgccagta
    42601 attttcacat cagtgaacca ctgacgatgg ccctgctgct tacgatagtg tttacgacga
    42661 cgaaacttaa cgattttaac tttctcgcca cgaccgtgag caacaacttc agctttgatt
    42721 acgccgccat caacgaaagg aacgccgatt ttgacttctt caccgtttgc gatcatcagc
    42781 acttcagcga actcaacagt ttcgccagtt gcgatgtcca gcttttccag gcgaacggtc
    42841 tgaccttcgc ttactcggtg ttgtttacca ccactttgga aaaccgcgta catataaaac
    42901 tccgcttccg cgcacacctt ttcaatgatt cagagtgcgc tataaatatt cacaataggg
    42961 cgcgaatatt acgcaaaacg cacgcctttg acaagtgcta cagtcaatac acgaagaaaa
    43021 aaacacaact tgtacggtaa cgtttatctg tgccattttt tcagtacaat taccctatat
    43081 tcctaaccat aaaccctaag ttgcctttgt tcacagtaag gtaatcgggg cgaaaagccc
    43141 ggcttttgcg atgaatttag aaaaaatcaa tgagttaacc gcgcaagata tggcgggtgt
    43201 taatgcggca atccttgagc agcttaattc cgacgtccaa ctgatcaatc agttaggcta
    43261 ttacatcgtc agcggcggcg gtaaacgtat tcgtccgatg attgctgtac tggctgcacg
    43321 agctgttggc tatgagggaa atgcgcatgt caccattgct gccctgatcg agtttatcca
    43381 cacggcgact ctgctacacg acgacgttgt ggatgaatca gatatgcgca ggggtaaagc
    43441 taccgccaac gccgcatttg gcaatgccgc cagcgtgctg gtaggcgatt ttatttatac
    43501 ccgcgcgttc cagatgatga ccagcctcgg ttcactcaaa gtgctggaag tcatgtcaga
    43561 agctgtaaac gtcatcgcag aaggtgaagt tctgcaactg atgaacgtta acgatccgga
    43621 catcactgaa gaaaactaca tgcgcgttat ctatagcaaa accgcgcgtc tgtttgaggc
    43681 tgccgcgcag tgttccggga ttctggctgg ctgtacgccg gaggaggaga aaggcctgca
    43741 ggattatggg cgctatctcg gcactgcttt ccagttgatc gacgatttac tcgattacaa
    43801 tgccgatggc gaacagttag gtaaaaacgt cggcgacgat ctgaacgaag gtaaaccgac
    43861 gctgccgctg ctgcatgcga tgcatcatgg cacaccagaa caggcacaga tgatccgtac
    43921 cgccatcgaa caaggtaacg gtcgccatct tctggaaccg gttctggaag caatgaacgc
    43981 ttgtggatct cttgaatgga cgcgtcagcg cgccgaggaa gaagcagaca aagccatcgc
    44041 agcgttacag gtgctcccgg acaccccttg gcgagaagca ctcatcggcc tcgcgcacat
    44101 cgctgttcaa cgcgatcgtt aatcccctcc cttcatcccg cgcagtgcgc gggatgattc
    44161 cagtaaattc cataaaaaca ctaattcagc gctataacac ctgaaaaaag ggcgataaat
    44221 ctttatatat tctgaatatt cacactcttt acaggaactt tttagcgcaa tagaccatca
    44281 ggagtatagt gatgcccgac agaagaagtg ttctgaatga aagcgactaa cttaaggagt
    44341 gaggaaagat ggaaagtaat ttcattgact ggcatcccgc tgacatcatt gcaggtttgc
    44401 gcaagaaagg aacgtcaatg gcggcggaat ctcgcagaaa tggtttgagt tcctcaacgc
    44461 tggcaaacgc attatcgcgc ccatggccga aaggagagat gattattgcg aaagccctgg
    44521 gaactgaccc ctgggttatc tggccatcac gctaccatga tccgcagacc catgagttta
    44581 tcgacagaac gcagttgatg cgtagctaca ctaaaccgaa aaaatgagtg gtctggcggt
    44641 agccccgcga acggggctgc cagctctcag acgattactc gcctttcaca cgctcaatat
    44701 ttgcacctaa agcgcgcagt ttgtcttcaa tgcgttcgta gccacgatcg atgtgataaa
    44761 tacgatcaac caccgtcgtc ccttccgcaa tacagccagc cagcaccagg cttgctgatg
    44821 cacgcagatc ggttgccata acctgtgcac cagaaagttt ttcaacacca tgacaaataa
    44881 cggtattgct ttcgatttcg gcgtgcgcgc ccatacggct cagctctggc acatgcataa
    44941 agcggttttc aaagaccgtt tcggtgatga acccggtccc ttcagccacc aggttcaaca
    45001 gcgtgaactg ggcctgcata tcggtcggga atgccggatg cggcgcggta cgtacgttaa
    45061 cagccttcgg acgtttgcca tgcatatcca ggctaatcca gtcttcgccg acttcgatgt
    45121 ccgctccagc atcacgcagt ttcgccagca cggcgtccag agtatctggc tgcgcgttac
    45181 ggcagataat tttgccacga gagatcgccg ccgccaccag gaaagtaccg gtttcgatac
    45241 gatccggcag cacgcgatag acaccgccgc ctaaacgttc cacaccttcg atgacgatac
    45301 gatcggtgcc ctgaccgcta attttcgcac ccagcgtaat caggaagttc gcggtatcga
    45361 cgatttccgg ttcacgcgct gcgttttcaa taatcgtggt gccttccgcc agggttgcag
    45421 cacacatgat ggtcaccgtt gcgccaacgc tgactttatc catcacgata tgtgcgcctt
    45481 tcagacgacc atcgacggaa gctttaacgt aaccttcttc cagtttgatg gtcgcgccta
    45541 attgttcgag gccagaaatg tgtagatcaa ccggacgcgc accgatcgta caaccgccag
    45601 gtagtgaaac ttgcccctga ccaaagcgcg ctaccagcgg ccccagcgcc cagatagaag
    45661 cacgcatggt tttaaccaga tcgtaaggtg cgcagaatac attaacgtcg cgggcatcaa
    45721 tatgcacaga accattacgt tctactttcg cacccagctg gcttagcagt ttcattgatg
    45781 tatcgacgtc tttcagtttc gggacgttct ggatctctac cggttcttcc gccagtagtg
    45841 cggcaaaaag aataggcaga gcagcatttt tagcgccgga aattgtgact tcgccctgga
    45901 gcttcgttgg cccctgaaca cgaaatttat ccatttagtt tgttctcagt taacaattca
    45961 tatccgctac cggcgaatcg cccatagctc aaaagccgtt cagtttgcga tcgcgcgccc
    46021 actccgcagg ggtatacgct ttgatcgaca cagcatgaat gcggttatcc gcaatatatt
    46081 ccatcagcgg gccatagacc gtctgctgtt ttttaacccg actcataccg tcaaacaact
    46141 cacccacggc aataacctga aagtggctgc catcgccgga aacgtggact tcctggaggg
    46201 agagagcgtt catcaacacg ctctgaattt cattattttc catgggatct tcaatcatca
    46261 gttaataaac cagcgaaaca tcttagagca aagttgcgct ggcataaata agcaaaaagc
    46321 ctcgctgata aatcagacaa ggctcgactt gcaggcaggt ttgccggaca ggcggttaac
    46381 gccatatccg gcctgaaaaa atttaacgag gcagaacatc agcaggcaaa ttatacaatt
    46441 tcgcgagggt atacactttg tcgtttaccc cctgaagcgt cacgttgttg ccctgctttt
    46501 tcgccagatc gataagatgg agcagcagtg ccagtccccc cgtatccacg cgggacacac
    46561 ggctaagatc gatgcaggta atccccttca ccgcctcctc acgcatttcc caaagcggta
    46621 gcagaacgtc ctgatccagc tctccggata acgccagcgt gtcacccgtc tgcatccagc
    46681 tcagtgactc gctcattatt ttttctcttc cagagtgatt ttctgttgag aaatcgattt
    46741 cagttgcgca gtcaggccgt cgataccttt ggtacgcagc agcgttcccc actcgttttg
    46801 tttggtggtg atcatactga cgccttcagc aatcatgtcg taagcttgcc agtttcccgt
    46861 ctgggagttt ttacgccact ggaagtccag acgcaccggc ggacggccat tcgggtcaat
    46921 aatggtaacg cgaataggca caatggtttt atcgcccagc ggctgttctg gcgcaatctg
    46981 ataggtttga ccgtgataca tcgccagcgc ctgaccgtaa gcctgcttca ggtactcacg
    47041 gaaagcggca aagtaggctt cacgttgagc aggggtcgca ctcttgtaat actggcccag
    47101 caccagcgca ccggcgtatt tcacctgtac gtatggcagc agttcctgat caacaatggt
    47161 acgcagataa tccgggttgg cccgaatttg cggttgctca ttcttcaggc gatcgaacgt
    47221 tttctgcgcc gcctcgtcca tcagcttata aggattggtc tggtctgccg cggttgccgc
    47281 actcagaggt gcaatcacca gcaaagcgac catcattaaa cgtttaaaca tgcgtcggtt
    47341 ctcctgaaat tatttcgttg tacccacagg ttcagtggtt tcattattac ctggcgcagc
    47401 agctggcgca tcgccactat tcttattgtc atcgccttta ctaccgtaaa ggaactgacc
    47461 aatgagatct tccagcacca tcgcagactt ggtgtcctga attgtatcgc catccttcag
    47521 gatagcagtc cccagttccg ggtcttcaaa accgacgttt aatgccagat attgttctcc
    47581 cagcaggccg gaagtacgaa tgctcagcga actggtatca ggaatgtggt tataacgttg
    47641 ttcaatttcc agcgttacgc gcggcagata ggttttcggg tccagcgtaa tatccgccac
    47701 ccggcccaca acaacaccac caatgctgac cggagagcgc gctttcaggc cgccaatgtt
    47761 atcgaacgtc gcataaagcg tgtaggtcga ttcagtacgt atggacgtca cgttcgccgc
    47821 cttcaggcaa acaaacagcg ccgccagcag tgctgctaat aaaaagatac ccacccaaat
    47881 ttcatttttt ttcgtttgca tgaactcaat tcccaaacat caatgcggtc agcacaaaat
    47941 ccagccccag aacagccaga gacgagtgga caacggtgcg agtggttgcc cggctaatcc
    48001 cggcagacgt cgggatggcg tcgtagccgt taaacaacga aatccacgtc accgtgatgg
    48061 cgaacaccac gctcttaatc agacagttga ccagatccat acgccagtcg acggcatttt
    48121 gcattgccga ccagaagaac ccgctatcaa tgcctttcca actgacgccg accagcgatc
    48181 cgccccagat ccccacggcg acgaagataa ccgtcaacag tggtaatgaa ataaccccag
    48241 cccagaaacg gggagaaata acccgacgca gcggatccac cgccatcatc tccatactgg
    48301 agagttgctc tgtagcgcgc atcaggccga tttctgcggt taacgccgaa ccagcacgcc
    48361 cggcaaacaa cagcgcggca accaccggcc ccagttcacg cagtagcgat aacgccacca
    48421 gcatacccag actggtttcc gcgctataag tggtcagaac cagataacct tgcagcccca
    48481 acaccattcc gatgaacacg ccagaaacta caataatcag catcgacagg acgccgacat
    48541 tatagagctg gcgcaccagc agcggcgcat gtttgcgaaa ttccggtttg ccgaccagcg
    48601 cattgaataa cattaacccg gcccgcccga acgttctcag ggttttaatc cctttatgtc
    48661 cgagcgacgc cagcgcattt aacagcatga gtggcttaac tccctggtaa aagatcagcg
    48721 tgataatcgc cggcaggata gcggaacgga acaggcccgt cagctatccc gtccagaaac
    48781 tgacgtacgc gcggatcagg attcgtctgc aacgcctggg cactgccatg agcgacaatt
    48841 tttttgtccg ccaggatcca ggcgtgatcc gcaatactta acacttccgg cacatcgtga
    48901 gaaaccacca cacaagtcac gcccagcgcg ctgttcagct cagaaatcag cttcaccagt
    48961 acgcccatgg tgatgggatc ttgcccaaca aaaggttcat caaacatgat gagatccggc
    49021 tccagcgcaa tcgcacgcgc cagcgctgca cgccgcgcca tcccaccgga aagttcagaa
    49081 ggcattagtt tagccgctcc acgcagcccc acggcctcca gcttcatcat caccgtacta
    49141 tgcaacaatg gcgcgggaag ttgggtatgt tcgcgcagtg gataggcgac gttgtcaaat
    49201 acgttcatat cagtgaacaa cgccccggac tgaaataaca tgctcatccg tttacgcact
    49261 gtatacaggc gcgaacgaga catcgccgga atattctcac catcgaaaag gatctcacca
    49321 tgatctggtg cgatttgccc gccaatcaga cggagtagcg tcgttttacc gatgcccgat
    49381 ggccccatga tcgccgtgat cttccctcgc ggcacggtca gggaaatatt atcgaagatg
    49441 cagcgattgc cacgcgtaaa actgacatcg cgcatatcga ctaaattcgc cacagactgc
    49501 tccataattc acccttcgtc ttgcgttgat tttctaagca tggcgctcaa tttaaccttg
    49561 aacccaacat atttacagaa tattacccgc cgtggttagc gaaagctggc atttgtttta
    49621 ctttttagcc gcataaagtc aaaattaaga atccgttacg gctttctgaa aatcttcagc
    49681 ggaccggcga gtatacctga agaaaggacg ttagatgctt ttagctacgg cactgttaat
    49741 tgttggttta cttttggtcg tttacagtgc cgaccgcctg gtttttgccg cgtctattct
    49801 ttgccgaacc tttggcatcc cgccgctgat catcggcatg acggtggtca gtattggtac
    49861 atcgttacca gaaatcatcg tctcgcttgc cgcgtctctg cacgaacaac gcgatttagc
    49921 cgttggtaca gccctcggct caaacattat caatatattg ctgatcctcg gtctggctgc
    49981 gctggttcgt ccttttaccg tccattccga tgttctgcgc cgtgaattac ccttaatgtt
    50041 gttggtcagc gtggtggccg gttccgtact ctatgacgga caacttagtc gaagcgatgg
    50101 tatctttctc ctctttctgg ctgtgctatg gctgctgttc attgttaaac ttgcacgtca
    50161 ggggactgac agcctgacca gagagcagct tgcagagctg ccgcgtgacg gcggattgcc
    50221 cgtcgcgttt ttatggctcg gcattgcgct tatcatcatg ccagtggcca cgcggatggt
    50281 ggttgataac gccacggtgc tggcgaatta ctttgccatc agcgagttga cgatggctct
    50341 gacggcaatt gctatcggaa ccagtctgcc ggaactggca accgcaatag cgggggttcg
    50401 caaaggtgaa aacgacattg ctgtcggaaa tatcattggc gcaaacattt ttaatattgt
    50461 catcgtgttg ggtttacccg cgctgataac gccaggagag attgatccac tggcgtacag
    50521 tcgtgactac agcgtgatgt tgctggtgag cattattttt gcgttgctgt gctggcggcg
    50581 ctccccgcaa ccgggccgtg gtgtaggggt attattaact ggcggattta tcgtatggct
    50641 ggcgatgttg tactggttat cgccaatact cgttgaataa ctggaaacgc attatgtcgc
    50701 acgtagagtt acaaccgggt tttgactttc agcaagcagg taaagaagtc ctggcgattg
    50761 aacgtgaatg cctggcggag cttgatcaat acatcaatca gaatttcacg cttgcctgtg
    50821 aaaagatgtt ctggtgtaaa gggaaagttg tcgtcatggg gatggggaaa tcggggcaca
    50881 tcgggcgcaa aatggccgca acgtttgcca gcaccggtac accttcattt ttcgtccatc
    50941 ctagtgaagc cgcgcatggt gatttaggca tggtcacccc acaggatgtg gtgattgcta
    51001 tctctaactc aggtgaatcc agcgaaatca cggccttaat tccagtgctt aagcgtcttc
    51061 acgtaccgtt aatctgcatc accggtcgcc cggagagcag catggcgcgc gccgcagatg
    51121 tgcatctgtg tgttaaagta gcgaaagaag cctgtccgtt agggctggca ccgaccagca
    51181 gcaccaccgc cacgctggtt atgggcgatg ccctcgctgt cgcgctgtta aaagcacgcg
    51241 gctttactgc tgaagatttt gcgctctcac acccaggcgg cgcactgggt cgtaaacttc
    51301 tgctgcgcgt aaacgatatt atgcatacgg gcgatgagat cccgcatgtt aagaaaacgg
    51361 ccagtctgcg tgacgcattg ctggaagtta cccgcaaaaa tcttggtatg actgtcattt
    51421 gcgatgacaa tatgatgatt gaaggcatct ttaccgacgg tgatttacgc cgtgtcttcg
    51481 atatgggcgt ggatgttcgt cagttaagta ttgccgatgt gatgacgccg gggggaatac
    51541 gtgtgcgccc tggcattctg gccgttgagg cactgaactt aatgcagtcc cgccatatca
    51601 cctccgtgat ggttgccgat ggcgaccatt tactcggtgt gttacatatg catgatttac
    51661 tgcgtgcagg cgtagtgtaa agattcaagg ataaacaaca atgagcaaag caggtgcgtc
    51721 gcttgcgacc tgttacggcc ctgtcagcgc cgacgttatg gcaaaagcag agaacattcg
    51781 tctgctgatc ctcgacgttg atggcgtact gtcagatggc ctgatttata tgggcaataa
    51841 cggcgaagag ctgaaagcgt tcaatgttcg tgacggttat ggcattcgtt gtgcgctcac
    51901 ctctgatatt gaagtcgcta tcattaccgg gcgaaaggct aaactggtag aagatcgttg
    51961 tgccacattg gggatcactc acttgtatca ggggcagtca aacaaactga tcgcctttag
    52021 cgatttgctg gaaaaattgg cgattgcccc ggaaaatgtg gcttatgttg gcgatgatct
    52081 catcgactgg ccggtaatgg aaaaagtggg tttaagcgtc gccgtggccg atgcgcatcc
    52141 actgttgatc ccgcgcgccg attacgtgac gcgcattgct ggcggtcgtg gcgcagtgcg
    52201 cgaagtttgc gacttattac tcctggcgca gggcaaactg gatgaagcca aagggcaatc
    52261 gatatgagta aagccagacg ttgggttatc attgtgctat cactggcggt tctggtgatg
    52321 atcggcatta atatggccga aaaagacgat accgcccagg tggtcgtcaa caacaatgat
    52381 cccacctata aaagcgagca tacggacacg ctcgtctata acccagaagg ggcactaagc
    52441 tatcgattga ttgctcaaca cgttgaatat tattccgatc aggccgtttc gtggtttacg
    52501 cagccggtac ttaccacgtt tgataaggat aaaatcccga catggtccgt aaaagcagat
    52561 aaagccaagc tgaccaatga ccggatgctc tatttatatg gacacgttga agtcaacgca
    52621 cttgtgccag actctcaact tcgcagaatc acgacggata acgcgcagat caatctggtg
    52681 acgcaggatg ttacctctga agacctcgtc acgttatacg gaacaacatt taactccagc
    52741 ggtctgaaaa tgcgcggcaa cttacgcagc aagaacgccg agctgattga aaaggttaga
    52801 acatcctatg aaattcaaaa caaacaaact cagccttaat cttgtgcttg ccagctcact
    52861 tctggccgcc agcattccgg catttgccgt aaccggagac actgatcagc cgatccacat
    52921 tgaatcggac cagcaatctc ttgatatgca aggcaacgtg gttaccttta ccggtaatgt
    52981 catcgtcacc cagggcacca tcaaaattaa tgccgacaaa gtggtcgtta cccgtccggg
    53041 cggcgaacaa ggtaaagaag tgattgacgg atacggtaaa ccggcaacgt tctaccagat
    53101 gcaggacaac ggtaaacccg ttgaaggtca cgcttcccag atgcactacg aactggcaaa
    53161 agattttgtc gttctgacgg gtaatgctta tctgcagcag gtcgatagca acattaaggg
    53221 cgataagatc acttacctgg tgaaagagca gaaaatgcag gctttcagcg acaaaggcaa
    53281 gcgcgtaacc accgttctgg tgccgtcgca gctgcaggac aaaaacaaca aaggccagac
    53341 cccggcacag aagaagggta attaattcgt tatggcaaca ttaactgcaa agaaccttgc
    53401 aaaagcctat aaaggccgtc gcgtggtaga agacgtcagc ctgaccgtca actccgggga
    53461 aattgtcggt ctgctggggc caaacggtgc cggtaagacc accactttct acatggttgt
    53521 aggcattgtg ccgcgcgatg cgggcaacat cattattgat gatgacgata tcagtctgct
    53581 gcctctgcat gcacgcgcgc gccgcggtat cggctatctg ccacaggaag cctccatttt
    53641 ccgtcgcctc agcgtttacg ataacctgat ggcggtactg caaattcgtg acgacttgtc
    53701 tgctgaacaa cgtgaagacc gcgcgaacga gctgatggaa gagtttcaca ttgagcacct
    53761 gcgtgacagc atggggcagt cactctccgg gggtgaacgt cgccgtgtag aaattgcccg
    53821 cgcactggct gcgaatccga aatttattct gctcgacgaa ccgtttgccg gggttgaccc
    53881 gatctcggtt atcgacatta aacgcatcat tgaacacctg cgcgacagcg gcctgggcgt
    53941 gctgatcact gaccacaacg tgcgtgaaac actggcggtt tgtgaacgcg cttatatcgt
    54001 cagtcagggg catttgatct cccacggcac gcctacagaa atcttacaag acgaacacgt
    54061 taagcgtgta taccttgggg aagacttcag actctgatag ggtagaagtt tgcgacgttt
    54121 tagcaggaga gtacgattct gaacatgaag caaggtttgc aactcaggct tagccaacaa
    54181 ctggcgatga cgccacagct ccaacaggca attcgtctgt tgcagttgtc gacgctggaa
    54241 cttcagcagg agctacagca ggcgctggag agtaatccgc tgcttgagca aatcgacact
    54301 catgaagaaa tcgacacccg cgaaacgcaa gacagtgaaa cgctggacac cgccgacgcg
    54361 ctcgaacaaa aagagatgcc ggaagagctg ccgctcgatg ccagttggga caccatttac
    54421 accgctggta caccatccgg caccagcggt gactacattg acgacgagct gccggtctac
    54481 cagggcgaaa cgacgcagac cttgcaggat tacctgatgt ggcaggtcga gctgacaccg
    54541 ttttccgaca ctgaccgcgc tattgctacc tctatcgtcg atgccgttga tgaaaccggt
    54601 tatctgactg tcccgctgga agatattctc gaaagtatgg gcgatgaaga gatcgacatc
    54661 gacgaggttg aagccgtcct taagcggatc caacggtttg acccggtcgg tgtagcggca
    54721 aaagatctgc gtgactgcct gctaatccaa ctctcccaat tcgataagac cacgccgtgg
    54781 ctggaagagg ccagattgat cattagcgat catctcgatc tgttagccaa tcacgacttc
    54841 cgcactttaa tgcgcgtcac gcgtctgaaa gaagatgtgc tgaaagaagc cgtcaatctg
    54901 atccagtcgc tcgatccgcg ccccgggcag tcgatccaga ctggcgaacc tgagtatgtc
    54961 attccagatg tgctggtgcg taagcataac ggtcactgga cggtagaact caacagtgac
    55021 agcattccgc gtctgcaaat caaccagcac tacgcctcga tgtgcaataa cgcgcgcaac
    55081 gatggtgaca gccagtttat ccgcagcaat ctgcaggatg ccaaatggtt gatcaagagt
    55141 ctggaaagcc gtaacgatac gctactgcgc gtgagtcgct gtatcgttga acagcagcaa
    55201 gccttctttg agcaaggtga agaatatatg aaaccgatgg tactggccga tatcgcccag
    55261 gctgtcgaaa tgcatgaatc gacgatatct cgcgtgacca cgcaaaaata cctgcatagt
    55321 ccacgaggca tttttgaact gaagtatttc ttttccagtc acgtcaatac cgagggcggc
    55381 ggcgaagctt cctccacggc gattcgtgca ctggtgaaga aattaatcgc ggcggaaaac
    55441 ccagcgaaac cgttgagcga cagcaagtta acctctttgc tgtcggaaca aggtatcatg
    55501 gtggcacgcc gcactgttgc gaagtaccga gagtctttat ccattccgcc gtcaaaccag
    55561 cgtaaacagc tcgtttgacc caaccgataa ggaagacact atgcagctca acattaccgg
    55621 aaataacgtc gagatcaccg aggcactgcg cgaatttgtt acagctaaat ttgccaaact
    55681 tgagcaatat tttgaccgaa tcaaccaggt ctatgttgtt ctgaaagtgg agaaagtcac
    55741 ccacacctca gatgcaacac tgcatgtaaa cggtggtgaa attcatgcca gcgcggaagg
    55801 tcaggacatg tacgctgcca ttgatggctt aattgataag ctggcacgcc agttgaccaa
    55861 acataaagat aaactgaaac aacactaatt gtccgggcaa ttagcatgtg catggcggcc
    55921 tgttgtgcgg cacaacgggc catttgtacg gttaatgctc cgagcctgtt ccactgtttg
    55981 agtgggcagg ttcttaggtg aaattatgac aaataatgat acaactctac agcttagcag
    56041 tgttcttaac agggaatgta cgcgaagccg cgtccactgt cagagcaaaa aacgcgccct
    56101 ggaaatcatc agcgagctgg cggcgaaaca acttagcctg ccgcctcagg tggtttttga
    56161 agctatcctg acgcgggaaa aaatgggcag taccggtatc ggcaatggta ttgccattcc
    56221 gcatggcaaa cttgaagaag atactctgcg tgccgttggc gttttcgttc agctcgaaac
    56281 gcctatagct ttcgatgcca tcgacaacca accggtggac ttactttttg ccctgctggt
    56341 gccggcagac caaactaaaa cgcacctgca tactctgtcg ctggtggcga aacgtctggc
    56401 ggacaaaacc atctgccgcc gtttacgcgc agcccagagc gatgaagagc tgtatcaaat
    56461 cattacggat accgaaggta ctccggatga agcgtagtta ttcggtaatg tctcttttag
    56521 acgttgtgag gagaaacagt acatggtact gatgatcgtc agcggacgtt caggttcagg
    56581 taaatctgtc gccctgcgtg cgctggaaga tatgggtttt tactgcgtgg ataaccttcc
    56641 cgtagtgttg ttacccgatc tggctcgaac tctggccgat cgcgagattt ctgccgccgt
    56701 cagcattgat gttcgtaata tgccggagtc accagaaata ttcgaacagg cgatgagtaa
    56761 cctgcctgac gctttctcac cgcaactact gttcctggat gccgaccgta ataccttaat
    56821 tcgtcgttac agtgacacgc gccgactgca tccgctttcc agcaaaaacc tgtcgctgga
    56881 aagtgctatc gacaaagaaa gcgatttgct ggagcctctg cgttcgcgag cggatctgat
    56941 tgtcgatacc tcagaaatgt ccgttcacga gctggctgaa atgctgcgta cccgtctgct
    57001 gggtaaacgt gaacgcgaac tgaccatggt ctttgagtct ttcggcttca aacacggtat
    57061 ccctatcgat gcagattacg tctttgacgt gcgcttcttg ccgaacccgc actgggatcc
    57121 gaaactgcgt ccaatgacag gtcttgataa acctgtcgcc gcgttcctcg accgccacac
    57181 agaagtacac aattttatct accagacgcg aagctatctt gagctatggt tacctatgct
    57241 ggaaaccaac aaccgtagct acctgacggt cgccattggt tgtaccggcg ggaagcaccg
    57301 ttcggtgtat attgcagagc aactggcaga ctacttccgc tcgcgcggta aaaacgtcca
    57361 gtcacgccat cgtacgctgg aaaaacgtaa accatgaccg tcaagcaaac tgttgaaatc
    57421 acaaacaagc tgggcatgca tgcccggcct gcaatgaagc tgtttgaatt aatgcagggt
    57481 tttgacgctg aagtgctgtt acgtaatgac gaaggcaccg aggctgaagc caacagcgtt
    57541 attgcgctgc tgatgttgga ttctgccaaa ggacggcaga ttgaagttga agcgaccggt
    57601 ccacaggaag aggaagcact ggccgccgtt atcgccctct ttaattctgg ttttgatgaa
    57661 gattaatctt catcactttg acatacaact cccttcaagc tcccccgaca ataagaaaat
    57721 cacgtactga aatcgttctc aatcaacgtc atttgtacat tttgtgcgct tttcactttc
    57781 agaagaacct taagaaaacc ttaagaggca ttgtttaggt tttgtttaag ttaatcgacc
    57841 atactggaga tcgtcagaaa atatttccag gagatggcat gattcgctta tctgaacaaa
    57901 gtcccctggg caccggaaga catcggaaat gttatgcgca tccggaagat gcccaacgct
    57961 gtatcaagat tgtctaccat cgtggtggcg ataaagagat ccgccgtgag ttaaagtact
    58021 acgcgcatct tggtcgccgg ttaaaagact ggagtggaat accgcgctat cacggtaccg
    58081 ttgagacgga ttgcggaacc ggttatgtct acgatgtgat tgccgatttt gacggcaagc
    58141 cttccattac gctgaccgaa tttgctgaac aatgccgtta cgaagaagac attgcacaac
    58201 tgcgccagct actgaaacag ctgaagcgtt atttgcagga taaccgtatc gtgacgatgt
    58261 cgctgaagcc gcagaatatc ctctgtcacc gcatcagtga gtcagaggtt atcccggtcg
    58321 tctgcgacaa cattggtgaa agcacattga ttccactggc gacctggtcg aagtggtgtt
    58381 gtctgcgtaa gcaagagcgg ttatggaaac gatttatcgc ccaaccggcg ctggctattg
    58441 ccctgcagaa agatctgcaa cctcgtgaga gcaaaacgct ggccctcact tcgcgcgaag
    58501 cttaatccag ctggtgttgt tgcataaacg gctcaccgcc taactgatac atctgccgta
    58561 aaatccacgc ctgacggcta cgcacgtagc ccgatggcga ggagactttg aaacgaagtg
    58621 gattaggtaa tacagctgcc agtaatgcag cttccgaccg ggtaagtttg ctcgcgggtt
    58681 tgtggaaata acgttgtgcc gcagcttcga cgccaaacac accgtcgcca aattcggcga
    58741 tattcaggta aaccgtcagg atacgctttt tgctccagac tgtttctatc cccagcgtta
    58801 atccagcttc cagccctttt cgtacccagc tacgcccatc ccataaaaag agattttggc
    58861 cgtctgctga gaaatcgttg aagcaccgcg aatacggttt tcattgcgct cgttgtgcgc
    58921 cagggctttc tcaatggaag cgacatcaaa gccccagtgc tcaggaaatt tctgatcttc
    58981 tgcggcaatc accgccagtc ccatccacgg cgagatttga tccatactga cccagtcaga
    59041 atgtgccacg taacgaaaat tgccatgcag ccaggcgctg acctgtcgct cgaccattac
    59101 cgctgagaag ggaacaggcg caacgctaaa caacgcgatg cccccgcccc agaaaacggc
    59161 gaggacaacc attaaccgca aaagaaaacg gcgaacgaaa ctaaacaccg ttaagcggct
    59221 tttactcatt cagccagaac cagcacgcgg gaaaccagct tatcaatgcc gctcgccgct
    59281 tctgcaatgt tctgcgccag catatatgct ggggtggtga caatcttatt gtcttcatca
    59341 accacgatat catcgacagg acacggcaca tgctccgcgc ccatctcttc cagcacttct
    59401 gcggtatcga tatcagtacc gatggtcaaa cgcagcggga aatcgaaaat tttcggcagc
    59461 atcgccgggg caatacacat aaaaccaagc ggttttccgg cctgatgcat cgcttgtgcc
    59521 agcgccttta attcacggtc aacggtgcat tcgctaccaa gactggcaaa attgcttaaa
    59581 ttcttcgccg cgccaacccc ccccggcaca atcaacgcat ccagttcagc ggcatcggcc
    59641 tgggccagag gacggatttc accgcgcgtt attcgtgccg cttcaatcag cacattgcgc
    59701 gtttccgtca tcgcttcgcc agttaaatgg ttgataacat caacctgctg cttatccggt
    59761 gcaaagcaga ccgcctgcgc accgctgcgt gaaatagcta acaacgtcaa caccgcttca
    59821 tgaatttcag aaccgtcata gacgccgcat ccgctcagaa ttacgccaat tttcttcatt
    59881 gtgatcatcc tttcgcaatt gactgaaaca cattaatttt tttaataaaa atggtacgca
    59941 tcacacattt aactgattca tgtaacaaat catttaagtt ttgctatctt aactgcgtgc
    60001 ggcctgaaaa acagtgctgt gcccttgtaa ctcatcataa taatttacgg cgcagccaag
    60061 atttccctgg tgttggcgca gtattcgcgc accccggtct agccggggtc attttttagt
    60121 ggcttttgcc acccacgctt tcagcacttc tacgtcgtga cgccactctt ctttcatctc
    60181 ttcaatccat tcaccgacgt tatcttccca ggcgggaagg tcaggagact gaatttgctg
    60241 acccagctgt tgcagatggc gtaaccccac tgaccccgcc gcacctttaa ttttatgtcc
    60301 ttcctcaaca atgccttttt tatcctgcgc cgtcagattc aactccagca cgctgacata
    60361 gcccggcatc atcttctcaa acaccgctaa cccgtcggtg atcagcttcg gtcctacaag
    60421 ttcgagatac tgttccagca tgggaatatc gagcaatgct tctgatttac tgttctcttc
    60481 tgtcgtcacc gtactctcct catcatcctg ggtatcccag aatttcttga tcatcgcggt
    60541 tagcgccgga accgaaagcg gcttactcag cacatcatcc attccagcat tgaggtactc
    60601 ttgtttgtct ttcagcacgt tagcggttaa ggccaccagc ggcggtaaat cctcgcgcgg
    60661 ataacgcttc gtcagttcac gagagatatc cagcccggtc atatctggca actgaatatc
    60721 cagcaacacc aggtcgtatt cgcccggttt aaacatctcc agcgccgcct tgccggtcat
    60781 ggcgacatca acgctgttac ctaatttttc cagcacagaa cgcgcgacaa tcacgttcag
    60841 ttcaatgtct tccaccagca gcacattcag cgccggtaaa ggcatatcgt cttcatcaaa
    60901 cgcatcatcg acctcttctg ccaccgacgg tgcgtggatc gtcaacgtaa aggttgaacc
    60961 tttgccctgt tcactggtaa ccgtaatatc gccgcccata tttttcgcca gacgacgaga
    61021 aacggccaga ccaataccgg tgccggtggc aggtttaccg ccatgactgt ctttcacctg
    61081 gtaatacatg gcgaagattt tatccagctc atcctgcgga atgccgatac cagaatcttc
    61141 cacttcaaaa tgcagcatat cgccttcatc gtagcgcacg cgcacggtaa cctggccttg
    61201 ctgggtgaat ttgacggcgt tactgatgag gttccacagg atctgccgta aacgcgtccc
    61261 gtcggtaatg acctgatgcg gtaatggcag cgtaggctcc aggttaaagc gcaatccttt
    61321 ttgttgcgcc tgcaatgcgg agagattttc cagatcggca aggaagctgg tgaaatcaac
    61381 cggctgatta tcaagctgga ccttgcgccg ttccatctta tccatgtcga taatatcgtt
    61441 aaagatattc cccagcgtga cggccgaaac atggatagtt ttgagatatt tttcctgctc
    61501 ggcggtgagt tcggtatcca gcagaatgcg gctcaggccg acgataccgt tcagcggtgt
    61561 acgcaattcg tgactgatgg tggaaataaa cgtcgttttg tcgcggctgg cgcgttcaag
    61621 cgcatcctga taccgcttac gctcggtaat gtcgcgacca aagcccatca aaccgtgacg
    61681 tttacccacg cggtcgtagt acggcacttt acggatttca aagcaggctt tgcgcccgtc
    61741 cgggtaatcc agccactgtt cataggtcag tgacacatta tgacggaaca ctttttcatc
    61801 ggtttcaatg acttttgcgg cggcttccgg tgagtaaaca tcagcaggtt tcaggtgaac
    61861 cagttgtttt tcgctttttc cggtcagcag ctccatcgcg cggttacagc cggaaaactc
    61921 tttatcttcg ttacgataaa aaaccaagtc gggtgaagca tcaaggaagg aacgtaagaa
    61981 tgaggattgc tgctcgagct gaatttgtgt ctcttcgcgc tctttgattt caattttcag
    62041 ttggccgaag gtttcctgca gttctgcttc cgctttttca cgaacggcaa tttcctgatt
    62101 tagctgggca atattatctt ttaactgaac gttgaggctc aaatcgcgct cgcgcatctc
    62161 ctccagtttt tgcaccagcc gtgacagacg ttgtcgtgac tcctccagtt gctcgacgac
    62221 caccgataga aagtagaccg cccacggcgt aatcagcaaa ccaaagaaga tagaacgaat
    62281 aacatcaatg ctttcgacct gaccatgcag caccatggtt accgccattt gtaccacaat
    62341 ggcaagaacg acgagggcca gcgccagcaa cattgagaag cgcaccagac ctaacttcat
    62401 catcaggtca acataatact gcgccagcag acgaatttgc ttcattaggg aattccttca
    62461 cgacaacctg ccccaataat acccaattct gacagttacg ttataaattg tgtgagaaat
    62521 gcggaattcc cctcaacgac ctactccgtt ggaggtagcc acggacgtcc cagcgccgat
    62581 ccctgtacgc catgttcatt cagatagcga tccagctcga ccattcccgt ccatcgattt
    62641 tcgcaccaca gcggagcaag cagcgtcggg cgacgggcgc tggcggaaat gcggtggtag
    62701 atcacttccg gtggcgtatg gcgaatcatt tctccggcag tgagcgtata atcctccagt
    62761 tcaataccgt tcaaacgcac cgcttcccag gctttcgcca taatgctgcc tttcacaata
    62821 tgcagcggat gcagctttat gccgtccacg ctggtttcaa ccactcgctc cagcgtttgc
    62881 aaacattcag cctgcccttc gccaggcagg ccgacaatta agtgggaaca caccttcagc
    62941 ccgcgctgac gtgccagctg ggttgtacgc tgataacagg caaaatcatg gccgcggttg
    63001 atgcgatgca gtgttttgtc gtgggcggtt tgtagcccca gctccagcca cacttcgtaa
    63061 ccctggtcct tatattcgca aagcagatcc agcaccgcat ccggcacgca gtccgggcgg
    63121 gtaccaacac acaaaccgac aatattggcc tggctcaccg cctgctgata catagaacgc
    63181 agcacctgaa cttccgcaaa ggtgctggta tacgcctgaa agtaggccag atagcgttta
    63241 gcgcggttaa ctaaattcgc ctggtgcgcc agttgctcgg caatggaacg atgctgctgc
    63301 gcttcatcgg caaacgaggc aacattacag aatgtgcagc caccacgccc gatggtaccg
    63361 tcacggttag ggcagctaaa accgccgtgc agcgtcagct tatgcacctt ttgcccataa
    63421 cgacgggtga gatcaccacc aaacatattg actaatttct gtaactgcat aatctgatag
    63481 accgcgcctt gaaaagaggc caaagcctgc catttttagc tcaattcggc gatgacctgg
    63541 gtcaatcgtc ccggcctgct tttatcaact gcataatcaa tcaaaattac cgaaatttca
    63601 tgcacaatca cataaatcac ttttgcttat cttgtgtcag atttttttat ctccttatgg
    63661 attttaggca aaaacagtag catgaaacgt cattaccaat taaggcagta taaaatgctg
    63721 gttttctcgt cagttcaagg caggataagg gttaacacgc ctttatgaca gtcaggaatt
    63781 gactgtttct ctaacgactc tccttttagc cttaaagata aaatctattt taatttcagc
    63841 tatttaataa agaattttgc gctaaagcac atttctgtac caataagctt gccatttgac
    63901 ctgtatcagc tttcccgata agttggaaat ccgctggaag ctttctggat gagcagcctg
    63961 ctcatcatat ttatgcagta attgagatcc cctcttcacc gtattaaccg atgcgaaaag
    64021 gacaacaagg gggcgaatgc gaggcgcgcg tatgacacgc aaaccccgtc gccacgctct
    64081 ttctgtgccc gtgcgcagcg gttcggaagt ggggttcccg cagagcctgg gggaggttca
    64141 cgatatgttg tacgataaat cccttgagag ggataactgt ggtttcggcc tgatcgccca
    64201 catagaaggc gaacctagcc acaaggtagt gcgtactgca atacacgcac tggcccgcat
    64261 gcagcaccgt ggcgcgattc tcgccgatgg taaaaccggc gacggttgcg gcttgctgtt
    64321 acaaaaaccg gatcgctttt ttcgcatcgt tgcgcaggag cgcggctggc gtttagcaaa
    64381 aaactacgct gtcgggatgc tcttcctgaa taaagatcct gaactcgccg ctgccgcacg
    64441 ccgcatcgtt gaagaagaac tgcaacgcga aaccttgtcg attgtgggct ggcgtgatgt
    64501 ccccactaac gaaggcgtgc tgggtgaaat cgccctctcc tctctgccac gcattgagca
    64561 aatttttgtg aacgccccgg caggctggcg tccacgcgat atggagcgcc gtctgtttat
    64621 cgcccgccgc cgcattgaaa agcgtctcga agccgacaaa gacttctacg tctgtagcct
    64681 gtcgaatctg gtgaacatct ataaaggtct gtgtatgccg gcggatctgc cgcgctttta
    64741 tctggatctt gcggacctgc gtctggaatc ggccatttgc ctgttccacc agcgcttctc
    64801 cactaacacc gtaccgcgct ggccgctggc gcaaccgttc cgctatctgg cgcataacgg
    64861 tgaaatcaac accatcaccg gtaaccgcca atgggcgcgt gcgcgtacct ataaattcca
    64921 gacgccgctg atccctgacc tgcacgacgc cgcgccattc gtcaacgaaa ccggttctga
    64981 ctccagttcc atggataaca tgctggaact gctgctggca ggcgggatgg atatcatccg
    65041 cgccatgcgc ctgttagtgc cacccgcctg gcagaacaac ccggatatgg acccggaact
    65101 gcgtgccttc tttgacttta actccatgca tatggagccg tgggatggtc cggcgggcat
    65161 cgtgatgtcc gatggtcgct tcgctgcctg taacctcgac cgtaacggtc tgcgtccggc
    65221 acgctacgtc attaccaaag acaagctcat cacctgcgcc tctgaagtcg gtatctggga
    65281 ttaccagcct gacgaagtgg tcgaaaaagg ccgcgtcggg ccaggcgaac tgatggtgat
    65341 cgacactcgc agtgggcgta ttctgcactc ggcagaaacc gatgacgatc tgaaaagccg
    65401 ccacccttat aaagagtgga tggagaaaaa cgtccgccga ctggtaccgt ttgaagatct
    65461 gcccgatgaa gaagtgggta gccgcgaact ggacgacgac acgcttgcca gctaccagaa
    65521 acagtttaac tacagcgcgg aagagctgga ctccgtaatt cgcgtactgg gcgaaaacgg
    65581 tcaggaagcg gtcggttcga tgggcgatga taccccattc gccgtgctct ccagtcagcc
    65641 gcgcattatt tacgactact tccgccagca gtttgcccag gtgactaacc cgccaatcga
    65701 cccgctgcgt gaagcgcatg ttatgtcgct cgccaccagt atcggtcgtg aaatgaacgt
    65761 cttttgcgaa gcagagggcc aggcgcaccg tttaagcttt aaatcgccga ttctgctcta
    65821 ctccgatttc aaacagctca cgacgatgaa agaggagcac taccgcgcag atacgctgga
    65881 tatcaccttt gacgtcacta aaaccacgct cgaagcgaca gtcaaagagc tatgcgacaa
    65941 agccgaaaaa atggtacgta gcggcaccgt gttgctggtg ctctccgacc ggaatatcgc
    66001 taaagatcgc ctgccggttc cggctccgat ggcggttggc gcgatccaga cccgtctggt
    66061 cgatcaaagc ctgcgttgcg atgccaacat catcgtcgaa accgccagcg cccgcgatcc
    66121 gcaccacttc gccgtgttgc tgggtttcgg cgcgacggct atttatccgt accttgccta
    66181 tgaaacgctg ggccgcctgg tagacaccca cgcgattgcc aaagattatc gtaccgtgat
    66241 gctcaactac cgtaacggca tcaacaaagg cttgtacaaa atcatgtcca aaatgggcat
    66301 ctccaccatc gcctcttacc gctgctcgaa actgtttgaa gcggtcggtc tacacgatga
    66361 tgtagtgggc ctgtgcttcc agggcgcggt cagccgcatt ggtggggcaa gctttgaaga
    66421 cttccagcag gatctgctga atctgtcgaa acgtgcctgg ctggcgcgca agccgattag
    66481 ccagggcggc ctgctgaaat acgtccacgg cggcgaatac cacgcctaca acccggacgt
    66541 ggtgcgcacg ctgcaacagg cggtacaaag cggcgagtac agcgactatc aggaatacgc
    66601 gaagctggtt aatgagcgtc cggcaaccac gctgcgcgat ctgctggcaa ttacgccggg
    66661 tgaaaacgcg gtcaacattg ctgatgttga accggcaagc gaactgttta aacgctttga
    66721 taccgccgcg atgtctatcg gcgcgttaag cccggaagcc cacgaggcgc tggcggaagc
    66781 gatgaatagc atcggcggta actcgaactc cggtgaaggc ggcgaagacc cggcgcgcta
    66841 tggcactaac aaagtgtcgc gcatcaagca ggtggcttct ggtcgcttcg gcgttactcc
    66901 ggcgtatctg gtcaatgccg acgtcattca gattaaagtc gcccagggcg cgaagccggg
    66961 cgaaggtggt cagttgccgg gcgacaaagt cactccttac atcgccaaac tgcgctattc
    67021 ggtgcccgga gtgacgctga tctccccgcc gccgcaccac gatatctact ctatcgaaga
    67081 cttagcgcag cttattttcg acctcaagca ggttaacccg aaagcgatga tctccgttaa
    67141 gctggtgtcc gaaccaggcg ttggcaccat cgcgactggc gtggcgaaag cttatgcgga
    67201 cttgatcacc atcgcgggct atgacggcgg caccggcgca agcccgctgt cgtcagtgaa
    67261 atatgcaggc tgcccgtggg aactcggtct ggtggaaacc caacaggcgc tggttgccaa
    67321 cggcttgcgt cataaaattc gtttgcaggt tgatggcggc ctgaaaacgg gtgtcgatat
    67381 catcaaagcg gcgattctcg gcgcagaaag cttcggcttc ggcactggcc caatggtggc
    67441 gctcggctgt aaatatctgc gtatttgcca cctgaacaac tgcgccacgg gtgtagcaac
    67501 tcaggatgac aaactgcgta agaatcacta tcacggcctg ccgttcaagg tgactaatta
    67561 ctttgagttt atcgcccgtg aaacccgcga gctgatggca cagctgggag taacgcgtct
    67621 ggtggatctg attggtcgca ccgacctgct gaaagagctg gacggtttca ccgccaaaca
    67681 gcagaaactg gcgctgtcga agctgctgga gactgccgag ccacatgcag gtaagtcact
    67741 ctactgcact gaaaacaacc cgccgtttga taacggccta ctgaacgcgc agttgctgca
    67801 acaggcgaaa ccgtttgtcg atgaacgcca gagcaaaacc ttctggttcg atatccgcaa
    67861 caccgaccgt tctgtcggcg cgtcgctttc aggctatatc gcccagacgc acggcgatca
    67921 ggggctggca gccgatccta tcaaagcgta cttcaacggc accgcaggcc agagtttcgg
    67981 tgtgtggaac gcgggcggcg tggaactgta cctgaccggg gatgccaacg actatgtcgg
    68041 taaaggcatg gcgggcggct taatcgccat tcgtcctccg gttggttccg ccttccgcag
    68101 ccatgaagca agcattatcg gcaacacctg cctgtatggc gcgaccggtg gtcgtctgta
    68161 tgccgcaggc cgcgcgggtg aacgtttcgg cgtgcggaac tccggtgcta tcaccgtggt
    68221 agaaggcatt ggcgacaacg gctgtgaata tatgacgggc ggtattgtct gtattctggg
    68281 taaaaccggc gttaacttcg gcgcgggcat gaccggcggc ttcgcttacg ttctcgatga
    68341 aagcggtgat ttccgcaaac gcgttaaccc ggaactggtc gaagtcttaa gcgtcgacga
    68401 tctggcgatc catgaagagc atctgcgcgg tcttatcacc gagcatgttc agcataccgg
    68461 ttctcagcgc ggtgaagaga ttctggcgaa ctggtcaacc ttcgccacga aattcgcgct
    68521 ggttaaaccg aagtccagtg atgtaaaagc actgctgggt caccgtagtc gtagcgcagc
    68581 agagttgcgc gtgcaggcgc agtaaggggt agcaacaatg agtcagaatg tttatcaatt
    68641 tatcgacctg cagcgcgttg atccgccaaa gaaaccgctg aagatccgta aaattgagtt
    68701 tgttgaaatt tacgagccgt tttccgaagg ccaggccaaa gcgcaggctg accgctgtct
    68761 gtcgtgcggc aacccatact gcgagtggaa atgcccggta cacaactaca tcccaaactg
    68821 gctgaagctc gccaacgagg ggcgtatttt tgaagcggca gaactgtcgc atcagaccaa
    68881 cacactgccg gaagtatgcg gacgtgtctg cccgcaagac cgtctgtgcg aaggttcctg
    68941 caccctgaac gatgagttcg gtgcggtgac catcggcaac attgagcgtt atatcaatga
    69001 taaagcgttc gaaatgggct ggcgtccgga tatgtccggc gtgaagcaaa ccggtaaaaa
    69061 agtggcgatt atcggtgcag gcccggcagg tctggcgtgt gcggatgtcc tgacgcgtaa
    69121 cggcgtaaaa gccgttgtct tcgaccgtca tccagaaatt ggcgggctgc tgaccttcgg
    69181 tattccggcc ttcaagctgg aaaaagaggt aatgacgcgt cgccgtgaaa tcttcacagg
    69241 catgggtatt gaattcaaac tcaataccga agtgggccgc gacgtacagc tggacgatct
    69301 gctgagtgat tacgatgccg tgttccttgg cgttgggact tatcagtcaa tgcgcggcgg
    69361 gctggaaaac gaagacgccg atggcgtgta cgcagcgctg ccgttcctta tcgccaacac
    69421 caaacagtta atgggctttg gtgaaacccg cgacgaaccg ttcgtcagca tggaaggcaa
    69481 acgcgtggta gtacttggcg gtggcgacac tgcgatggac tgcgtgcgca cgtctgtacg
    69541 ccagggggca aagcacgtta cctgtgccta tcgtcgtgat gaagagaaca tgccgggttc
    69601 ccgccgcgaa gtgaaaaacg cgcgggaaga aggcgtagag ttcaaattca acgtccagcc
    69661 actggggatt gaagtgaacg gtaacggcaa agtcagcggc gtaaaaatgg tacgtaccga
    69721 aatgggcgaa ccggatgcca aaggccgtcg ccgcgcggag atcgttgcag gttccgaaca
    69781 tatcgttccg gcagatgcgg tgatcatggc gtttggtttc cgtccacaca acatggaatg
    69841 gctggcaaaa cacagcgtcg agctggattc acaaggccgc atcatcgctc cggaaggcaa
    69901 cgacaacgct ttccagacca gcaacccgaa aatctttgct ggcggcgata tcgtccgtgg
    69961 ttccgatctg gtcgttaccg ccatcgccga aggtcgcaaa gcggcagacg gcattatgaa
    70021 ctggctggaa gtttaagctc tgtcccagcg ccgcctcctc agaaaagagt cggcgctgaa
    70081 attctccttt ctttctggaa atcgcctcgc ataatctgaa gaaaaccaat gtaatacgct
    70141 catctggatt gttaccggta gatgcccggc gtatagctga ttccattatt caacgcagcg
    70201 ccatataaag ggacagataa tggaatcgct ctcagagaga acctcaacag gctaccagca
    70261 aatccacaac ggcattattc atctggtcga tagcgcccga acggaaacgg tacgtagcgt
    70321 taacgcgctg atgaccgcga cgtactggga aattggccga cggattgtcg aatttgaaca
    70381 aggtggtgag gccagagcag cttatggtgc acagttgatt gatcggctat cgaaggattt
    70441 aagtcgacgg tataaacgtg gggtctctgc aaaaaactta cgccaaatga ggctttttta
    70501 cctctttttt caacatgttg aaattcgcca gacagtgtct ggcgaattaa caccattggg
    70561 aattccgcag acaccatctg cagaattccc atcggcaaag atttggcaga cgctgtctgc
    70621 caaattgaga ggctgtttga tatttccaca gcttttgtat tgcatctcct attttcggtt
    70681 tgtatattca tgcaaataaa cgaag
//
LOCUS       DAJTQY010000008        67100 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.008, whole genome shotgun sequence.
ACCESSION   DAJTQY010000008 DAJTQY010000000
VERSION     DAJTQY010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 67100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67100
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.008"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(64..1068)
                     /gene="cra"
                     /locus_tag="OO349_000536"
     CDS             complement(64..1068)
                     /gene="cra"
                     /locus_tag="OO349_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HCR5865729.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(1150..1236)
                     /locus_tag="OO349_000537"
     CDS             complement(1150..1236)
                     /locus_tag="OO349_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HCR5865730.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(1248..1739)
                     /gene="ilvN"
                     /locus_tag="OO349_000538"
     CDS             complement(1248..1739)
                     /gene="ilvN"
                     /locus_tag="OO349_000538"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCR5865731.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(1742..3466)
                     /gene="ilvI"
                     /locus_tag="OO349_000539"
     CDS             complement(1742..3466)
                     /gene="ilvI"
                     /locus_tag="OO349_000539"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HCR5865732.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVCPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRALQCLKYDTHSEKIKPQAVIETLWRLTNGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            3585..3629
                     /locus_tag="OO349_000540"
     CDS             3585..3629
                     /locus_tag="OO349_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5865733.1"
                     /translation="MKRNDKILHYEGLN"
     gene            complement(3784..4728)
                     /gene="leuO"
                     /locus_tag="OO349_000541"
     CDS             complement(3784..4728)
                     /gene="leuO"
                     /locus_tag="OO349_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HCR5865734.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            5388..5474
                     /gene="leuL"
                     /locus_tag="OO349_000542"
     CDS             5388..5474
                     /gene="leuL"
                     /locus_tag="OO349_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HCR5865735.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            5567..7138
                     /gene="leuA"
                     /locus_tag="OO349_000543"
     CDS             5567..7138
                     /gene="leuA"
                     /locus_tag="OO349_000543"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HCR5865736.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVTNYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKTQHNENNKETV"
     gene            7138..8229
                     /gene="leuB"
                     /locus_tag="OO349_000544"
     CDS             7138..8229
                     /gene="leuB"
                     /locus_tag="OO349_000544"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HCR5865737.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLNAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDIQCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            8232..9632
                     /gene="leuC"
                     /locus_tag="OO349_000545"
     CDS             8232..9632
                     /gene="leuC"
                     /locus_tag="OO349_000545"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HCR5865738.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            9643..10248
                     /gene="leuD"
                     /locus_tag="OO349_000546"
     CDS             9643..10248
                     /gene="leuD"
                     /locus_tag="OO349_000546"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HCR5865739.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIASYEAKQPAFMN"
     gene            complement(10297..11475)
                     /gene="setA"
                     /locus_tag="OO349_000547"
     CDS             complement(10297..11475)
                     /gene="setA"
                     /locus_tag="OO349_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HCR5865740.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGDWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSDLGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVVAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNTISTGVILAGVIQGAIAQSWGHFAVYWVIAIISVVALFLTAKVKDV
                     "
     gene            complement(11577..11708)
                     /gene="sgrT"
                     /locus_tag="OO349_000548"
     CDS             complement(11577..11708)
                     /gene="sgrT"
                     /locus_tag="OO349_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HCR5865741.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            11797..13452
                     /gene="sgrR"
                     /locus_tag="OO349_000549"
     CDS             11797..13452
                     /gene="sgrR"
                     /locus_tag="OO349_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HCR5865742.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIT
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPCEWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEKKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      13486..13585
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            13616..14599
                     /gene="thiB"
                     /locus_tag="OO349_000550"
     CDS             13616..14599
                     /gene="thiB"
                     /locus_tag="OO349_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HCR5865743.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            14575..16185
                     /gene="thiP"
                     /locus_tag="OO349_000551"
     CDS             14575..16185
                     /gene="thiP"
                     /locus_tag="OO349_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HCR5865744.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            16169..16867
                     /gene="thiQ"
                     /locus_tag="OO349_000552"
     CDS             16169..16867
                     /gene="thiQ"
                     /locus_tag="OO349_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HCR5865745.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTEELL
                     SGKASASAILGITG"
     gene            complement(16981..17745)
                     /locus_tag="OO349_000553"
     CDS             complement(16981..17745)
                     /locus_tag="OO349_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5865746.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(17831..18709)
                     /gene="araC"
                     /locus_tag="OO349_000554"
     CDS             complement(17831..18709)
                     /gene="araC"
                     /locus_tag="OO349_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HCR5865747.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGCHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPYFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQR
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            19048..20748
                     /gene="araB"
                     /locus_tag="OO349_000555"
     CDS             19048..20748
                     /gene="araB"
                     /locus_tag="OO349_000555"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HCR5865748.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDTPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            20759..22261
                     /gene="araA"
                     /locus_tag="OO349_000556"
     CDS             20759..22261
                     /gene="araA"
                     /locus_tag="OO349_000556"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HCR5865749.1"
                     /translation="MAIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PYKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLPVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            22461..23156
                     /gene="araD"
                     /locus_tag="OO349_000557"
     CDS             22461..23156
                     /gene="araD"
                     /locus_tag="OO349_000557"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HCR5865750.1"
                     /translation="MLKDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            23231..25582
                     /gene="polB"
                     /locus_tag="OO349_000558"
     CDS             23231..25582
                     /gene="polB"
                     /locus_tag="OO349_000558"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HCR5865751.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IHADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPLLLEKLNAWFANHDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDIIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRQTIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            25747..28653
                     /gene="rapA"
                     /locus_tag="OO349_000559"
     CDS             25747..28653
                     /gene="rapA"
                     /locus_tag="OO349_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HCR5865752.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSSEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKSCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQELAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHTILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            28665..29324
                     /gene="rluA"
                     /locus_tag="OO349_000560"
     CDS             28665..29324
                     /gene="rluA"
                     /locus_tag="OO349_000560"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HCR5865753.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(29441..30256)
                     /gene="djlA"
                     /locus_tag="OO349_000561"
     CDS             complement(29441..30256)
                     /gene="djlA"
                     /locus_tag="OO349_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HCR5865754.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            30511..32865
                     /gene="lptD"
                     /locus_tag="OO349_000562"
     CDS             30511..32865
                     /gene="lptD"
                     /locus_tag="OO349_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HCR5865755.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKFLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYHYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASRPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            32918..34204
                     /gene="surA"
                     /locus_tag="OO349_000563"
     CDS             32918..34204
                     /gene="surA"
                     /locus_tag="OO349_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HCR5865756.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            34204..35193
                     /gene="pdxA"
                     /locus_tag="OO349_000564"
     CDS             34204..35193
                     /gene="pdxA"
                     /locus_tag="OO349_000564"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HCR5865757.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDASIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            35190..36011
                     /gene="rsmA"
                     /locus_tag="OO349_000565"
     CDS             35190..36011
                     /gene="rsmA"
                     /locus_tag="OO349_000565"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HCR5865758.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            36014..36391
                     /gene="apaG"
                     /locus_tag="OO349_000566"
     CDS             36014..36391
                     /gene="apaG"
                     /locus_tag="OO349_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HCR5865759.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGTIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            36398..37240
                     /gene="apaH"
                     /locus_tag="OO349_000567"
     CDS             36398..37240
                     /gene="apaH"
                     /locus_tag="OO349_000567"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HCR5865760.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(37318..37797)
                     /gene="folA"
                     /locus_tag="OO349_000568"
     CDS             complement(37318..37797)
                     /gene="folA"
                     /locus_tag="OO349_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HCR5865761.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(37988..39850)
                     /gene="kefC"
                     /locus_tag="OO349_000569"
     CDS             complement(37988..39850)
                     /gene="kefC"
                     /locus_tag="OO349_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HCR5865762.1"
                     /translation="MDSHTLVQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLIENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(39843..40373)
                     /gene="kefF"
                     /locus_tag="OO349_000570"
     CDS             complement(39843..40373)
                     /gene="kefF"
                     /locus_tag="OO349_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HCR5865763.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(40421..41815)
                     /locus_tag="OO349_000571"
     CDS             complement(40421..41815)
                     /locus_tag="OO349_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5865764.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMC
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGSLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMGLFVQISL
                     FSESILLTISRVNI"
     gene            complement(41874..42161)
                     /gene="fixX"
                     /locus_tag="OO349_000572"
     CDS             complement(41874..42161)
                     /gene="fixX"
                     /locus_tag="OO349_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HCR5865765.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQVLELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(42158..43444)
                     /locus_tag="OO349_000573"
     CDS             complement(42158..43444)
                     /locus_tag="OO349_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR5865766.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVLLAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGNPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(43495..44436)
                     /locus_tag="OO349_000574"
                     /pseudo
     CDS             complement(43495..44436)
                     /locus_tag="OO349_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
     gene            complement(44451..45221)
                     /locus_tag="OO349_000575"
     CDS             complement(44451..45221)
                     /locus_tag="OO349_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HCR5865767.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAVDA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            45693..47207
                     /gene="caiT"
                     /locus_tag="OO349_000576"
     CDS             45693..47207
                     /gene="caiT"
                     /locus_tag="OO349_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiport BCCT
                     transporter"
                     /protein_id="HCR5865768.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVMGLTASTWILWTVLGSNTLLLMDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            47238..48380
                     /gene="caiA"
                     /locus_tag="OO349_000577"
     CDS             47238..48380
                     /gene="caiA"
                     /locus_tag="OO349_000577"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HCR5865769.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            48509..49726
                     /gene="caiB"
                     /locus_tag="OO349_000578"
     CDS             48509..49726
                     /gene="caiB"
                     /locus_tag="OO349_000578"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HCR5865770.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            49800..51353
                     /gene="caiC"
                     /locus_tag="OO349_000579"
     CDS             49800..51353
                     /gene="caiC"
                     /locus_tag="OO349_000579"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HCR5865771.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGNKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESE
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYM
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQAQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPS
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            51462..52247
                     /gene="caiD"
                     /locus_tag="OO349_000580"
     CDS             51462..52247
                     /gene="caiD"
                     /locus_tag="OO349_000580"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HCR5865772.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPTIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            52253..52843
                     /gene="caiE"
                     /locus_tag="OO349_000581"
     CDS             52253..52843
                     /gene="caiE"
                     /locus_tag="OO349_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HCR5865773.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFSGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(52929..53324)
                     /gene="caiF"
                     /locus_tag="OO349_000582"
     CDS             complement(52929..53324)
                     /gene="caiF"
                     /locus_tag="OO349_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HCR5865774.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(53586..56807)
                     /gene="carB"
                     /locus_tag="OO349_000583"
     CDS             complement(53586..56807)
                     /gene="carB"
                     /locus_tag="OO349_000583"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HCR5865775.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     DRLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(56825..57973)
                     /gene="carA"
                     /locus_tag="OO349_000584"
     CDS             complement(56825..57973)
                     /gene="carA"
                     /locus_tag="OO349_000584"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HCR5865776.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRIDKPAFSFQGHPEASPGPHDAAPLFDHFIKLIEQYRKTAK"
     gene            complement(58429..59250)
                     /gene="dapB"
                     /locus_tag="OO349_000585"
     CDS             complement(58429..59250)
                     /gene="dapB"
                     /locus_tag="OO349_000585"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HCR5865777.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNNL"
     gene            complement(59417..60331)
                     /gene="rihC"
                     /locus_tag="OO349_000586"
     CDS             complement(59417..60331)
                     /gene="rihC"
                     /locus_tag="OO349_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HCR5865778.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLLQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALVP"
     gene            complement(60397..61347)
                     /gene="ispH"
                     /locus_tag="OO349_000587"
                     /gene_synonym="lytB"
     CDS             complement(60397..61347)
                     /gene="ispH"
                     /locus_tag="OO349_000587"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HCR5865779.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKHAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(61349..61798)
                     /gene="fkpB"
                     /locus_tag="OO349_000588"
     CDS             complement(61349..61798)
                     /gene="fkpB"
                     /locus_tag="OO349_000588"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR5865780.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(61885..62379)
                     /gene="lspA"
                     /locus_tag="OO349_000589"
     CDS             complement(61885..62379)
                     /gene="lspA"
                     /locus_tag="OO349_000589"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HCR5865781.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVLLAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            complement(62379..65195)
                     /gene="ileS"
                     /locus_tag="OO349_000590"
     CDS             complement(62379..65195)
                     /gene="ileS"
                     /locus_tag="OO349_000590"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HCR5865782.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWLPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(65238..66179)
                     /gene="ribF"
                     /locus_tag="OO349_000591"
     CDS             complement(65238..66179)
                     /gene="ribF"
                     /locus_tag="OO349_000591"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HCR5865783.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(66187..66405)
                     /locus_tag="OO349_000592"
     CDS             complement(66187..66405)
                     /locus_tag="OO349_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HCR5865784.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGQFLPVICSMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            66508..66771
                     /gene="rpsT"
                     /locus_tag="OO349_000593"
     CDS             66508..66771
                     /gene="rpsT"
                     /locus_tag="OO349_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HCR5865785.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
BASE COUNT        16291 a        18260 c        17266 g        15283 t
ORIGIN      
        1 tgtccagtcc cgtactctac gcgccagagg gaaattcacc tggcgcgtat ttttgttcgc
       61 ggattagcta cggctgagca cgccgcggcg atagagatta cgtttaatgc gcgttaaacc
      121 aggttttggc ttacgcggtt cgtccaggct tgccaggaca atctccagca cacgctctgc
      181 gacatcgcgg tgacgttgag ccactgccag caccggacac tgtaagaagt cgagcagttc
      241 gttatcgcca aaggtggcaa ttgccaggtc agaaggcagt ttgccgtcgc gacgcagcgt
      301 gacatccatc actccttgca acaacgcaaa cgacgttgtg aacagcgcct gcggcatcgg
      361 atgcgtttcc agccattttt cgaataactg ggcagccgcc tcccgctcat agctgttggc
      421 atacaggaaa tgcacttcgc gcggatcatc tttccaagca gtacggaaac cttgttcacg
      481 caggaagctg acagaaagct ccggcagcgc gccaagataa agcaccgtct cggcgggaaa
      541 cttacgtaac tcttccgcca gcatttcggc atcatcctga tcggcaccaa ccacgctggt
      601 gaaatgttca cgatcgaggg cgcggtccag cgcgacaatc gggaacgggt cgttagccca
      661 gcgttgataa aaaggatgct caggtggcaa cgacgtcgaa acaataatgg catcaacctg
      721 acgctgtaaa aggtgctcaa tgcagcgcat ttcgttgtct ggctgatctt ctgagcaggc
      781 aatcagcagt tgataacccc gttgccgcgc ctggcgttca agatagttag cgatgcgggt
      841 atagctggtg ttctccagat cggggatcac aagaccaata gaacgtgtgc gtccagcacg
      901 aagcccagct gccacggcgt tcgggtggta attgtgctca cgcaccacag ccatgacttt
      961 ttcaacggtt ttgtcgctca cacggtattg cttcgctttg ccgttaataa catagcttgc
     1021 agtggtccgc gacactcccg ccagccgagc gatttcatcc agtttcacaa ttgccccttg
     1081 cgtaaaatgt aaaaaccata gccattgtac tggtatgggt taaattcatt aacatctaag
     1141 cgcagaaaat taactgcggc aactgctttt attccaggtt cccactgatt tcgcaaaaaa
     1201 agcccaacgt gacatgttgg gctgcaaatt gcgcactgag atcattctca acgcattatt
     1261 ttatcgccgc gcgaaagtcc gaccacacca gagcgagcaa cttccacaat tttcgccaca
     1321 tcgcgaatcg atgctaaaaa tgcatcaagc ttatcgctgg tgcctgctaa ttgaacggta
     1381 taaagcgagg gggtgacatc gataatttgc ccacggaata tttccgtatt acgtttcact
     1441 tcgtcacgcc cgtaaccgct ggcctgaatt ttcaccagca tgatttcccg ctcaacatgc
     1501 gcgccctgcc ccaactcact cacgcgcaag acatcgacca gcttgtgtaa ttgcttttcg
     1561 atctgctcaa gtactttttc atcgcccacg gtctggatgg tcatacgcga taatgtcgga
     1621 tcgtcggttg gcgcaacggt caggctttca atgttgtagc ctcgctggga aaaaaggcca
     1681 atcacgcggg ataacgcgcc tgattcattt tcgagtaaga ctgataatat ccggcgcata
     1741 atcaggttct ctccgttttg cttaaccaca tttcatccat tccgcccccg cgaatctgca
     1801 tcgggtagac gtgctcactg ccatcgacgg taacatcaac aaacaccagg cgattattgc
     1861 gcacctgttc cagcgcctcg ctaagtttgc tttccagctc atgcggatga gagatctgga
     1921 tcccgacgtg accataggct tccgccagac ggacgaaatc gggtagtgat tgcatataag
     1981 attgtgaatg acggccggaa tagatcatgt cctgccactg tttcaccatc cccagatagc
     2041 ggttattgag attcaccacc agtacgggca actcgtattg caacgcggta gacagttcct
     2101 ggatgttcat ctgaatactg ccgtcgccag tgacgcaaac cacggtttct tctggcaacg
     2161 ccattttgac gcccagtgcc gcaggtaaac caaaacccat cgtgccgaga ccgccggaat
     2221 tgatccagcg acgcggtttg tcgaatggat aataaagcgc agcaaacatc tggtgctgcc
     2281 cgacatcgga cgtcacgtaa gcgtccccat tagtcaaccg ccaaagagtc tcgatcaccg
     2341 cctgcggttt aatcttttca ctgtgagtgt catatttcag gcactgaaga gcgcgccact
     2401 gttcaatctg ctgccaccag tcgcggatct catccagcgg ttgatgggcg gattcttgcg
     2461 acaggagttc aagcatttgt tcgaggacct ggcgagcatc ccccacaatc gggatatccg
     2521 cagtcacggt tttagaaatg gaagtaggat caatatcgat atgcagaaca gtggcatttg
     2581 ggcagtactt tgccagattg ttcgtcgttc ggtcatcaaa tcgtaccccg acggcgaaaa
     2641 tcacatccgc gttatgcatc gtcatattgg cttcgtaggt accgtgcatt cccagcatgc
     2701 ccagtgcctg acgatgcgtt gccggaaacg cccccagccc catcaatgag gaaacaacgg
     2761 gcagattcaa cgcctccacc gtttctttca actgctgatg gcagcccgcc atgattgccc
     2821 caccgcctac gtagacaacc ggtttttttg ccgctaccag cgtttgcaga gcacgcttaa
     2881 tttgcccttt atgtccggta gtagtgggat tgtaagaacg catactgacc gactcagggc
     2941 agacataggg taatttgttc gccggattaa gaatatcttt cggtaaatca acgaccactg
     3001 gtcctgggcg accacttgcc gccagccaga aagccttttt cagcacctgc ggaatgtctt
     3061 ccgtttgctt aaccagaaaa ctgtgtttaa ccaccggtcg cgaaatcccc accatgtcgc
     3121 actcctgaaa ggcatcgtaa cctatcaacg aggtcgctac ctgcccggaa aggacaacta
     3181 atggaatgga atccatataa gcggtggcga tgccggtaat cgcattggtc gcccctggac
     3241 ccgacgttac cagcacgacg ccgacttccc cggtcgcacg cgccaggcca tcggccatat
     3301 gcaccgccgc ctgctcatga cgaactaata catgatcaat accacccacg gtatgcaatg
     3361 catcgtaaat atcaaggact gcgcctccgg gataaccgaa tacttgttta acgccctgat
     3421 cgataagcga tcggacgacc atctcggctc cagacaacat ctccatggcc tgcctcactg
     3481 tttgacggaa aaaatgtgta aaacacatta accgtccgat cttgcttaag caattggcag
     3541 ccagagacat aaacgcaggg taataagaat aaacaggagg aaaggtgaaa agaaacgata
     3601 aaatcctcca ttacgaagga ttaaattgat aataaatcgg ctgaatccca caacttacag
     3661 caatttctct atttttttaa ttaatcctaa aatagagagt ccacacattc agaataaaat
     3721 tctacattca ataaaaaatg cagaataaac cagacattca tgtctggcct attctgcatt
     3781 cagttagcgt ttgcaaattg agactaattg ttcttccatc cactgatggc ctttatcgcg
     3841 cccggcagct tcatgccaag agagataaca ggttctgctg ttttgtttta acggcagcgg
     3901 taatacctgt aattctaagg attcagcgaa ctcttcagcc agccaacgcg gcgcaatagc
     3961 gaccaaatgc gtttgcgaca ccacgctaag tacgctcatc attgccatgc cctgatacgc
     4021 gatactggct tgcttatcta ccgtgtcata ccaaggttga ctaaatgacg cgaaacgatc
     4081 gagcgaaacc gccgcatgtt gttcgttata aacatcatgt ttcagtaacg ggcctttaat
     4141 tgttggatga tttttgctgg ctaccagcac catttcatct ttaaataatg gcacgctggt
     4201 aaattcagga cgatggaagt cttcataact aatcacaaac tccgtttcct gataacgcag
     4261 ctgatgttca gtgttctgat ttaatgaaga cttgaacata acatgtatat ttggcgcaat
     4321 ctgctcaatg tgattataaa tctgcgaggt cagaatgctg tctaacgggc tgcaaacaca
     4381 aagatgaaat acacgttcac tgctcgcggg ttcaaaacct gaaccaggca attcattttg
     4441 tactagttgc aatgcctggc gaactgaacc aaaaagttga aatgcgcgag cagtcggttg
     4501 aataccacgg ccataacgaa caaaaagctc gtcattaaac atcaccttca ggcgtgcaac
     4561 agcgttactg accgcaggtt gcgacatacc cagaacatga gcggcacggg taatattttg
     4621 ctcctgcatc acggcatcga aaacggttaa taagttgaga tcgaccatgc gtagctgtgg
     4681 cttgcttaac tccgccgtct ctgaatgatc tgtttgtacc tctggcatac ttaactccac
     4741 tgtcacactt aactcccttt cccttattgg aatgcagaga aataatcctg aagaatatga
     4801 tttatcatgc atataaaata agaaaaagca aaatgagtaa aattcggaaa acataaagat
     4861 acgggttctg ataaaaacag aatccattgc cgtggaatga gtcatttacc acagcataat
     4921 aatccataat gtttttgttg cgaaaacaat ctaatcataa ctaccgcgaa tactcaatca
     4981 tctacaaaat ggattaaatg tgcttattta ataattaatt tatatattta atgcattaat
     5041 tcttaacatt aattgatcaa taatattcac caaatcaata tcaaaaaaaa tcgcaaaaca
     5101 tataattcaa tacaaatcat caggataggt tttgcaacgc gtgcattttg tccccttttt
     5161 cctcgttgat tagatgcaaa aatttatgct gaaatatgtc aaccgatgaa aagcgtcggt
     5221 agttaagcag aaattaatat cgcttacttt aaccaccgta gcacaattag ctaattttac
     5281 ggatgcagaa ctcacgctgg cgggacgttt ttattgcgtc agggttgaca tccgtttttg
     5341 tatccagtaa ctctaaaagc atatcgcatt catctggagc tgatttaatg actcacatcg
     5401 ttcgctttat cggtctacta ctactaaacg catcttcttt gcgcggtaga cgagtgagcg
     5461 gcatccagca ttaagccagc acgcagtcaa acaaaaaacc cgcgccattg cgcgggtttt
     5521 tttatgcccg aagcgaggcg ctctaaaaga gacaaggacc caaaccatga gccagcaagt
     5581 cattattttc gataccacat tgcgcgacgg tgaacaggcg ttacaggcaa gcttgagtgt
     5641 gaaagaaaaa ctgcaaattg cgctggccct tgagcgtatg ggtgttgacg tgatggaagt
     5701 cggtttcccc gtctcttcgc cgggcgattt tgaatcggtg caaaccatcg cccgccaggt
     5761 taaaaacagc cgcgtatgtg cgttagctcg ctgcgtggaa aaagatatcg acgtggcggc
     5821 cgaatccctg aaagtcgccg aagccttccg tattcatacc tttattgcta cctccccaat
     5881 gcacatcgcc accaagctgc gcagcaccct agacgaggtg atcgaacgcg ctatctatat
     5941 ggtgaaacgc gcccgtaatt acaccgatga tgttgaattt tcttgcgaag atgccgggcg
     6001 tacacccatt gccgatctgg cgcgagtggt cgaagcggcg attaatgccg gtgccaccac
     6061 catcaacatt ccggacaccg tgggctacac catgccgttt gagttcgccg gaatcatcag
     6121 cggcctgtat gaacgcgtac ctaacatcga caaagccatt atttccgtac atacccacga
     6181 cgatttaggc ctggcggtcg gcaactcact ggcggcggta catgccggtg cacgccaggt
     6241 ggaaggtgca atgaacggga tcggcgagcg tgccggtaac tgttcgctgg aagaagtcat
     6301 catggcgatt aaagttcgta aggatattct caatgtccac accgccatta atcaccaaga
     6361 gatatggcgc accagccagt tagttagcca gatttgtaat atgccgatcc cggcaaacaa
     6421 agccattgtt ggcagcggcg cattcgctca ctcctccggt atccaccagg atggcgtgct
     6481 gaaaaaccgc gaaaactacg aaatcatgac accagaatct attggtctga accaaatcca
     6541 gctgaatctg acctctcgtt cggggcgtgc ggcggtgaaa catcgcatgg atgagatggg
     6601 gtataaagaa agtgaatata atttagacaa tttgtacgat gctttcctga agctggcgga
     6661 caaaaaaggt caggtgtttg attacgatct ggaggcgctg gccttcatcg gtaagcagca
     6721 agaagagccg gagcatttcc gtctggatta cttcagcgtg cagtctggct ctaacgatat
     6781 cgccaccgcc gccgtcaaac tggcctgtgg cgaagaagtc aaagcagaag ccgccaacgg
     6841 taacggtccg gtcgatgccg tctatcaggc aattaaccgc atcactgact ataacgtcga
     6901 actggtgaaa tacagcctga ccgccaaagg tcacggtaaa gatgcgctgg gtcaggtgga
     6961 tattgtcacc aactacaacg gtcgccgctt ccacggcgtc ggcctggcca ccgatattgt
     7021 cgagtcctcc gccaaagcca tggtgcacgt actgaacaat atctggcgtg ccgcagaagt
     7081 cgaaaaagag ttgcaacgca aaactcaaca caacgaaaac aacaaggaaa ccgtgtgatg
     7141 tcgaagaatt accatattgc cgtattgccg ggagacggta ttggtccgga agtgatgacc
     7201 caggcgctga aagtgctgaa tgccgtgcgc aaccgctttg cgatgcgcat caccaccagc
     7261 cattacgatg taggcggcgc agccattgat aaccacgggc aaccactgcc gcctgcgacg
     7321 gttgaaggtt gtgagcaagc cgatgccgtg ctgtttggct cggtaggcgg cccgaagtgg
     7381 gaacatttac caccagacca gcaaccagaa cgcggcgcgc tgctgccgct gcgtaagcac
     7441 ttcaaattat tcagcaacct gcgcccggca aaactgtatc aggggctgga agcattctgt
     7501 ccgctgcgtg cagacattgc cgctaacggc ttcgacatcc agtgcgtgcg cgaactgacc
     7561 ggcggcatct atttcggtca gccaaaaggc cgcgaaggta gcggacaata tgaaaaagcg
     7621 tttgataccg aggtgtatca ccgttttgag atcgagcgta ttgcccgcat cgcgtttgaa
     7681 tctgctcgta aacgtcgcca caaagtcacc tcaatcgaca aagctaacgt gctgcaatcc
     7741 tctattttat ggcgtgaaat cgtcaacgag atcgccacgg aatacccgga tgtcgaactg
     7801 gcgcatatgt acatcgacaa cgccaccatg cagctgatta aagatccatc acagtttgac
     7861 gttctgctgt gctccaacct gtttggcgac attctgtctg acgagtgcgc aatgatcact
     7921 ggctcgatgg gcatgttgcc ttccgccagc ctgaacgagc aaggttttgg actgtatgaa
     7981 ccggcgggcg gctcggcacc agatatcgca ggcaaaaaca tcgccaaccc gattgcacaa
     8041 atcctgtcgc tggcactgct gctgcgttac agcctggatg ccgatgatgc ggcttccgcc
     8101 attgaacgcg ccattaaccg tgcattagaa gaaggcattc gcaccgggga tttagcccgt
     8161 ggcgctgccg ccgttagtac cgatgaaatg ggcgatatca ttgcccgcta tgtggcagaa
     8221 ggggtgtaat catggctaaa acgttatacg aaaaattatt cgacgctcac gtagtgtacg
     8281 aagccgaaaa cgaaaccccg ctgttatata tcgaccgcca cctggtgcat gaagtgacct
     8341 caccgcaggc gttcgatggc ctgcgcgccc acggtcgccc ggtacgtcag ccgggcaaaa
     8401 ccttcgccac tatggatcac aacgtctcta cccagaccaa agacattaat gcctgtggtg
     8461 aaatggcgcg catccagatg caggagctga ttaaaaactg caaagaattt ggcgtcgagc
     8521 tgtatgacct gaatcacccg tatcagggga tcgtccacgt aatggggccg gaacagggcg
     8581 tcaccttgcc ggggatgacc attgtctgcg gcgactcgca taccgccacc cacggcgcgt
     8641 ttggcgcact ggcctttggt atcggcactt ccgaagttga acacgtactg gcaacacaaa
     8701 ccctgaaaca gggccgtgcg aagaccatga aaattgaagt ccagggcaaa gccgcgccgg
     8761 gcattaccgc aaaagatatc gtgctggcaa ttatcggtaa aactggcagc gcaggcggca
     8821 ccgggcatgt ggtggagttt tgcggcgaag caattcgtga tttaagcatg gaaggtcgta
     8881 tgaccctgtg caatatggcg atcgaaatgg gcgcaaaagc cggtctggtt gctcctgacg
     8941 aaaccacctt taactatgtc aaaggccgtc tgcatgcgcc gaaaggcaaa gatttcgacg
     9001 acgccgttgc ctactggaaa accctgcaaa ccgacgaagg cgcaactttc gataccgttg
     9061 tcactctgca agcagaagaa atttcaccgc aggtcacctg gggcaccaat cccggccagg
     9121 tgatttctgt gaacgacaat attcccgatc cggcttcgtt tgccgatccg gttgaacgtg
     9181 cgtcggcaga aaaagcgctg gcctatatgg ggctgaaacc gggtattccg ctgaccgaag
     9241 tggctatcga caaagtgttt atcggttcct gtaccaactc gcgcattgaa gatttacgcg
     9301 cggcagcgga gatcgccaaa gggcgaaaag tcgcgccagg cgtgcaggca ctggtggttc
     9361 ccggctctgg cccggtaaaa gcccaggcgg aagcggaagg tctggataaa atatttattg
     9421 aagccggttt tgaatggcgc ttgcctggct gctcaatgtg tctggcgatg aacaacgacc
     9481 gtctgaatcc gggcgaacgt tgtgcatcca ccagcaaccg taactttgaa ggccgccagg
     9541 ggcgcggcgg gcgcacgcat ctggtcagcc cggcaatggc tgccgctgct gctgtgaccg
     9601 gacatttcgc cgacattcgc aacattaaat aaggagcaca ccatggcaga gaaatttatc
     9661 aaacacacag gcctggtggt tccgctggat gccgccaatg tcgataccga tgcaatcatc
     9721 ccgaaacagt ttttgcagaa agtgacccgt acgggttttg gcgcgcatct gtttaacgac
     9781 tggcgttttc tcgatgaaaa aggccaacag ccaaacccgg acttcgtgct gaacttcccg
     9841 cagtatcagg gcgcttccat tttgctggca cgagaaaact tcggctgtgg ctcctcgcgt
     9901 gagcacgcgc cctgggcatt gaccgactac ggttttaaag tggtgattgc gccgagtttt
     9961 gctgacatct tctacggcaa tagctttaac aaccagctgc tgccggtgaa attaagcgat
    10021 gcagaagtgg acgaactgtt tgcgctggtg aaagccaatc cggggatcca tttcgacgtg
    10081 gatctggaag cgcaagaggt gaaagcggga gagaaaacct atcgctttac catcgatgcc
    10141 ttccgccgcc actgcatgat gaatggtctg gacagcatcg gcctgaccct gcagcacgac
    10201 gacgctattg cgtcttatga agcaaaacaa cctgcgttta tgaattagac cccttgcccg
    10261 gtcaaatgac cgggctttcc gctatcgtcc acgtcatcaa acgtctttaa cctttgcggt
    10321 taaaaataat gcgacaacag aaataatcgc aattacccag tagacagcaa agtgccccca
    10381 actttgtgca attgctccct gaataacacc agccagaatt accccggtag agatagtgtt
    10441 ggtaaataag gtggtagctg cccccgctct tccaggcatt aaatcctgaa accatagcat
    10501 cccaataccc gcaacaatgc cgataaatac agcgttaaaa agttgcagcg tcatcaacgc
    10561 catacggcta tgaaagaaaa tcaatccagt gtaaaacagc actcctgcca ccactgcaat
    10621 gaccatcatt cgccgcttac cataacgttt gacatagtag ccagccagaa tcattgctgg
    10681 tatttccagt ccagctgccg tccccatcag gaaacccgcc agtttgtctg gcaatcctaa
    10741 atcgctactg atccacaatg gcatatcaat aatgtacata gtgttgcagg tccacattaa
    10801 cgtcgaggcg acaaataaca tccgtacgtt actatcctgc cagtcacctt gcattgataa
    10861 agcattttcc gacggcagtt ctacccgcgc cacagacgga agcataaatg caatcaatac
    10921 cagactgagt gtgaatatcc cggcggcaat cgaaaacatc actgtaaagc cgtaattcaa
    10981 cgccagcata aaggccagcg gtggaccgat tacccatgcc agagaaagct gcgcacgcat
    11041 caccgagcta aacatcacca cttctcgcgc cgagttatcc gcgtattccc gcgccagagc
    11101 aaataactgt ggcattgccg tattggccag agatgccaga agcacaccac aggtgataag
    11161 cgtcagataa tgacgattaa atgcaaacag tagcgcattg ccaatagcca tcaaacagca
    11221 aaatataatc agttttcgcc gatcgccctg actgtcagaa cgttttgcca accagaggct
    11281 tacgccgatc ccagcaatag cattcaccgt ataaaagagt ccgatccaga aaggttgcgc
    11341 gccaacctca cgactcagaa ataagctcaa tgtaggagcc tgtagcgccc cggccacccc
    11401 catcataaaa gcgaccagca taaatgccgc gtaaacaccg ttcatacggc gagccatcgt
    11461 cattatccag atcatacgtt ccctttttag cgcggcgaga ataaaaaaaa ccagcaggta
    11521 taatctgctg gcgggtgatt ttacaccaat actcagtcac acatgatgca ggcaagtcaa
    11581 ctttcagaat tgcggtcatc ccactgcatc agtccttcaa gcatggttaa tcgttgtggg
    11641 acgcttaacc aacgcaacca gcacaacttc gctgtcgcgg taaaatagtg ctgataaaac
    11701 tgacgcatgg ggcacctcct tgcttcatcg ttaggaatta ttgacttaat atagggaaaa
    11761 taaaattgct gtcttttgcg caggagttcc ccttttatgc catctgctcg tctgcaacaa
    11821 cagttcatcc gcctgtggca atgctgcgag ggtaaatcgc aggacacaac gctcaacgaa
    11881 ctggcagcgt tattgagctg ctcgcgtcgt catatgcgta ccctgctcaa caccatgcag
    11941 gatcgcggct ggctgacgtg ggaagcggaa gtcgggcgcg gtaaacgctc gcgtctgaca
    12001 ttcctctata ccgggctggc gcttcagcaa cagcgggcgg aagacctgct ggagcaggat
    12061 cgtatcgacc aactagtgca gttggttggc gacaaagcga ctgtgcggca aatgctggtt
    12121 tctcatctgg gccgcagctt ccgccagggg cggcacatcc tgcgcgtgct ctactatcgt
    12181 ccgttgcgta atctgctacc tggcagcgca ttgcgccgtt ccgaaaccca tattgcccgg
    12241 caaatcttca gttcgctaac gcgcataaat gaggaaaatg gggaactgga agcagacatc
    12301 gcccaccact ggcagcaaat atcaccgctt cactggcgtt tctttttgcg tccaggagtc
    12361 cattttcatc atggtcgtga actggaaatg gacgacgtga tcgcctcttt aaaacgaatc
    12421 aatacgctgc cgctctattc gcatattacg gacattgtgt cgccgacgcc ctggacgctg
    12481 gatatccatc tcacgcagcc ggaccgctgg ttgccgttac tgctggggca agttccggcg
    12541 atgatcctgc cgtgcgaatg ggaaaccctc agtaactttg ccagccatcc tattggcacc
    12601 ggtccgtatg cggtgattcg caacagcacc aatcaactga aaattcaggc attcgatgac
    12661 ttcttcggtt accgggcatt aatcgacgaa gttaacgtct gggttctgcc ggaaattgcc
    12721 gacgagccag ccggtggtct gatgctaaaa ggtccacagg gcgagaaaaa agagattgaa
    12781 agccgcctgg aggaaggttg ctactattta ctgttcgaca gccgcaccca tcgcggggcg
    12841 aatcagcaag tcagggactg ggtaagctat gtgctttctc caactaatct ggtctatttc
    12901 gctgaggaac agtaccagca actgtggttc ccggcttatg gactgctccc ccgttggcac
    12961 catgcccgca ccataaagag cgaaaaaccg gctggcctgg aaagcctcac cctgaccttt
    13021 tatcaggatc acagtgagca tcgggtgatt gccgggatca tgcagcagat tctggcaagt
    13081 caccaggtca cgctggaaat caaagagatc agctacgatc agtggcatga aggagagatc
    13141 gagagcgata tctggcttaa tagcgccaac tttacgctgc cgctggattt ttcgctgttc
    13201 gcgcacttgt gcgaggtgcc gctgctccaa cactgtattc cgatcgactg gcaagccgac
    13261 gccgcccgct ggcgcaatgg cgaaatgaac ctggcgaact ggtgccagca actggtcgcc
    13321 agtaaagcaa tggtgccgct tatccaccac tggctgatca ttcagggaca acgcagtatg
    13381 cgcggcctgc gtatgaatac cctcggctgg ttcgatttta aatcagcgtg gtttgcgcca
    13441 ccggatccat gattgctggt agataacaaa atcactacac taacgccgtt ctcaacgggg
    13501 tgccacgcaa acgcgtgcgc tgagaaaata cccgtcgaac ctgatccgga taacgccggc
    13561 gaagggattt gaggctcctt ctcaagtcct ttgccactct tttttgaggt gcaaagtgtt
    13621 aaaaaaatgt cttcccctgc tgttgctgtg cacagcgccc gttttcgcta aacccgttct
    13681 gactgtttat acctacgatt ccttcgccgc cgactggggg cctggtccaa agatcaaaaa
    13741 agcttttgaa gccgactgta actgcgaact gaaactggtg gcgctggaag atggcgtttc
    13801 gcttctcaac cgtctacgga tggaaggcaa aaatagtaaa gccgatgtgg tgctggggct
    13861 ggataacaat ctgttagacg ccgccagtaa aaccggacta tttgccaaaa gcggtgtggc
    13921 agcggatgcc gttaacgttc ccggcggctg gaataatgac actttcgtcc cgtttgatta
    13981 cggctatttc gccttcgtct atgacaagaa caaactgaaa aacccgccgc aaagcctgaa
    14041 agaattggtt gagagcgatc agaactggcg ggtgatttat caggatccgc gtaccagcac
    14101 gccggggctg ggtctgctgt tgtggatgca aaaagtctat ggcgatgacg ccccacaagc
    14161 ctggcaaaaa ctggcgaaga aaaccgtcac ggtcaccaaa ggctggagcg aagcctacgg
    14221 cctgttttta aaaggtgaaa gcgatctggt actgagttac accacctctc cggcttatca
    14281 cattctcgaa gagaaaaaag ataactacgc cgccgcgaac tttagcgaag gtcactatct
    14341 gcaggtggaa gtcgccgccc gcaccgctgc cagcaagcag ccggagctgg cgcaaaaatt
    14401 cctccagttt atggtttctc cggcttttca gaatgcgatc ccaaccggta actggatgta
    14461 tccggtggca aatgtcacgc tgcctgccgg gtttgaacaa ttgaccaaac cggccaccac
    14521 gctggagttc acaccagctg aagtggcggc acaacgtcag gcatggatta gcgaatggca
    14581 acgcgccgtc agccgttaat tcccggctgg ttaattccag gtgtaagcgc cgccacgctg
    14641 gtggtagcgg ttgcgctcgc ggcgtttctc gccctgtggt ggaacgcgcc gcagggtaac
    14701 tgggtggcag tctggcagga cagctacctg tggcatgtgg tgcgcttctc cttctggcag
    14761 gcgtttctct cggcgctact ctctgtcgta cccgcgatat tcctcgcccg cgccctctat
    14821 cgcaggcgct ttccgggtcg gctggcgctg ttgcgtctgt gcgcaatgac cttgatcctc
    14881 ccggtgctag ttgctgtttt cggcattctt agcgtctatg gtcgtcaggg ctggctggca
    14941 tcgctctgcc aatcgctcgg tctggagtgg accttttcgc cctacggcct gcaaggcatt
    15001 ttgctggcgc acgtattttt taatctgccg atggcgagcc gcttattact ccaggcactg
    15061 gaaaacattc ccggcgaaca acgtcagctt gccgcccagc ttgggatgcg cggctggcat
    15121 tttttccgct tcgtcgaatg gccgtggcta cggcgacaaa tcccgccggt tgccgcgctt
    15181 atctttatgc tctgtttcgc cagcttcgcc accgtgctat cgctgggcgg cggtccgcag
    15241 gcaaccacta tcgagctggc tatttatcag gcgctgagtt acgactacga tcctgcccgc
    15301 gcggcgatgc tggcgctgct ccagatggtg tgttgccttg ggctggtgct gttgagtcag
    15361 cgactaagta aggccattgc gcccggcacc acgctgctgc aaggctggcg cgatccggac
    15421 gatcgtctgc atagccgcat ttgcgacacg gtgttaatag tgctggcgct gctgctgttg
    15481 ctgccaccgt tgctggcggt gatcgtcgat ggggtaaatc gccagttgcc ggaagtgctg
    15541 gcacaaccgg tgctgtggca ggcgctgtgg acctcgttgc gtattgcgct ggcggcaggt
    15601 gtattgtgcg tagtgctgac catgatgctg ctatggagca gtcgcgaact gcgggcgcgg
    15661 cagaaaatgc tggcgggtca ggcgctggag atgagcggca tgttgatcct cgccatgccg
    15721 gggattgtgc tggctaccgg cttcttttta ctgctcaaca acactatcgg cctgccacaa
    15781 tctgctgacg gcattgtgat tttcaccaat gcgttaatgg cgatccctta tgcgctgaaa
    15841 gtactggaaa acccgatgcg cgatatcacc gcccgctaca gtatgttgtg tcagtcgctg
    15901 ggcattgaag gctggtcgcg cttaaaagtg gtcgagctgc gcgccctgaa acgtccactg
    15961 gcgcaggcgc tggcttttgc ctgcgtgctg tcgattggtg attttggcgt agtggcgttg
    16021 ttcggtaacg atgatttccg taccctgccg ttttatctct accagcaaat tggctcctat
    16081 cgcagccagg acggcgcggt caccgcgtta attctgctac tactctgttt tctgctgttt
    16141 accgtgattg aaaaactacc ggggcgaaat gttaaaactg actgatatca cctggcttta
    16201 tcaccatttg ccgatgcgtt ttagcttaac ggtagaacgc ggcgagcagg tggcaatcct
    16261 cgggccaagc ggcgcgggta aaagtaccct gctgaatttg attgccggtt ttctgacgcc
    16321 agccagcggt tcgctgacta tcgatggtgt tgatcacacc acgacgccgc cgtcacgccg
    16381 tccggtgtcg atgctgtttc aggagaacaa cctgttcagc catctgacgg tcgcgcagaa
    16441 catcgggctg gggctaaatc cggggttgaa actgaacgcg gcacagcagg agaaaatgca
    16501 cgcgattgcc cgtcagatgg ggattgataa tttaatggcg cggttaccgg gcgagctttc
    16561 cggcggtcag cgacagcgcg tggcgttagc gcgttgtctg gtgcgcgaac agccgatttt
    16621 attgctcgat gaaccgttct ctgcgctcga tccggcgtta cgtcaggaga tgctgacgct
    16681 ggtgagcacg agctgccagc agcaaaaaat gacgctattg atggtgtcgc acagtgtgga
    16741 agatgcggcg cggatcgcca cacgctcggt ggtggtcgcc gacgggcgca tcgcctggca
    16801 gggtaagact gaagaattgt tgagcggtaa ggcgagtgct tcggcaatat tggggattac
    16861 gggttagtat gcactgtcag atgcggcgtg aacaccttat ccgacctacg gacagtgcgt
    16921 tttgtaggcc tgataagacg cggtcagcgt cgcatgaggc aacccgcaca agacaatccc
    16981 ctaaacccca accactttac gcaaaatatc gatatacacc ggcatcaacg ggtggcgaat
    17041 taacaccacc agcgccacca cgccgatggc agaaatcaac ggcgtcagcc acaacaaacg
    17101 gccgcgcgac aaataatgac ttaaacgatc ggttgcttta ccgctgcgcc ataaccgcca
    17161 gcacagccag ccaccaaccc acaaaaatac cgccgtcgcc agcagcaacc atttaaactc
    17221 gccgctctgc attccggctg gaatatcgat cgccgcgccc gccagaatcc caggcaggaa
    17281 gtaaaacggt ggccacagca ggcagccgat aatattcggc gtaatgaatt ttgccaccgg
    17341 cagatccagc attcccgcca ccattggcac cagcggacgc gtcgggccaa caaagcgacc
    17401 gaccagaatg gtgaacatgc tgtgttgatg caacgcatgt tcagttttat cgagtaatgc
    17461 tttgtttttc ttcagaaatg accagcgatg caacggcttt ttaaaacgcc agcccagcca
    17521 gaaggagatc cagtcgccca gcagacaacc gacaatccct gccagccaag cgtgccagaa
    17581 acttaactcg ccgctgccaa tcagcgctcc cagccccgcc atcagcaccg taccgggtag
    17641 aatcaaaccg accagcgcca gcgactccag aaaggccacc aacaccaccg ccatcaatga
    17701 atacacggta gattgggtaa taaagtgttc cagcaatgct tgcataatgt gcctgtcaaa
    17761 tggacgaagc agggattctg caaaccctat gctactccgt caagccgtca attgtctgat
    17821 tcgttaccaa ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac
    17881 ggaactcgct cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat
    17941 cgtcaaaacc aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca
    18001 gcttcgcctg gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaaccgct
    18061 ggcggaaaag atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga
    18121 tatcaaaatt gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacacgat
    18181 tatccatcgg tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct
    18241 caagcagatt tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga
    18301 tttgcccaaa caggtcgctg aaatacggct ggtgcgcttc atccgggcga aagaaacccg
    18361 tattggcaaa tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt
    18421 aaacccactg gtgataccat tcgcgagcct ccggatgaca accgtagtga tgaatctctc
    18481 ctggcgggaa cagcaaaata tcacccggtc ggcagacaaa ttctcgtccc tgatttttca
    18541 ccaccccctg accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt
    18601 cgataaaaaa atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg
    18661 cattaaacga gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac
    18721 tcccgccatt cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg
    18781 tcttttactg gctcttctcg ctaacccaac cggtaacccc gcttattaaa agcattctgt
    18841 aacaaagcag gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca
    18901 gaaaagtcca cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat
    18961 ccataagatt agcggatcct acctgacgct ttttatcgca actctctact gtttctccat
    19021 acccgttttt ttggatggag tgaaacgatg gcgattgcaa ttggcctcga ttttggcagt
    19081 gattctgtgc gagctttggc ggtggactgc gccagcggtg aagagatcgc caccagcgta
    19141 gagtggtatc cccgttggca aaaagggcaa ttttgtgatg ccccgaataa ccagttccgt
    19201 catcatccgc gtgactacat tgagtcaatg gaagcggcac tgaaaaccgt gcttgcagag
    19261 cttagcgtcg aacagcgcgc agctgtggtc gggattggcg ttgacagtac cggctcgacg
    19321 cccgcaccga ttgatgccga cggtaacgtg ctggcgctgc gcccggagtt tgccgaaaac
    19381 ccgaacgcga tgttcgtatt gtggaaagac cacactgcgg ttgaagaagc ggaagagatt
    19441 acccgtttgt gccacgcgcc gggcaacgtt gactactccc gctatattgg cggtatttat
    19501 tccagcgaat ggttctgggc aaaaatcctg catgtgactc gccaagacag cgccgtggcg
    19561 caatctgccg catcgtggat tgagctgtgc gactgggtgc cagctctgct ttccggtacc
    19621 acccgcccgc aggatattcg tcgcggacgt tgcagcgccg ggcataaatc tctgtggcac
    19681 gaaagctggg gcggcttgcc gccagccagt ttctttgatg agctggaccc gatcctcaat
    19741 cgccatttgc cttccccgct gttcactgac acctggactg ccgatattcc ggtgggcacc
    19801 ttatgcccgg aatgggcgca gcgtctcggc ctgcctgaaa gcgtggtgat ttccggcggc
    19861 gcgtttgact gccatatggg cgcagttggc gcaggcgcac agcctaacgc actggtaaaa
    19921 gttatcggta cttccacctg cgacattctg attgccgaca aacagagcgt tggcgagcgg
    19981 gcagttaaag gtatttgcgg tcaggttgat ggcagcgtgg tgcctggatt tatcggtctg
    20041 gaagcaggcc aatcggcgtt tggtgatatc tacgcctggt ttggtcgcgt actcggctgg
    20101 ccgctggaac agcttgccgc ccagcatccg gaactgaaag cgcaaatcaa cgccagccag
    20161 aaacaactgc ttccggcgct gaccgaagca tgggccaaaa atccgtctct ggatcacctg
    20221 ccggtggtgc tcgactggtt taacggtcgt cgcacgccaa acgctaacca acgcctgaaa
    20281 ggggtgatta ccgatcttaa cctcgctacc gacactccgc tgctgttcgg cggtttgatt
    20341 gctgccaccg cctttggcgc acgcgcaatc atggagtgct ttaccgatca ggggatcgcc
    20401 gtcaataacg tgatggcgct gggcggcatc gcgcggaaaa accaggtcat tatgcaggcc
    20461 tgctgcgacg tgctgaatcg cccgctgcaa attgttgcct ctgaccagtg ctgtgcgctc
    20521 ggtgcggcga tttttgctgc cgtcgccgcg aaagtgcacg cagacatccc atcagcccag
    20581 caaaaaatgg ccagtgcggt agagaaaacc ctgcaaccgc gcagcgaaca ggcacaacgc
    20641 tttgaacagc tttatcgccg ctatcagcaa tgggcgatga gcgccgaaca acactatctt
    20701 ccaacttccg ccccggcaca ggctgcccag gccgttgcga ctctataagg acacgataat
    20761 ggcgattttt gataattatg aagtgtggtt tgtcattggc agccagcatc tgtatggccc
    20821 ggaaaccctg cgtcaggtca cccaacatgc cgagcacgtt gttaatgcgc tgaatacgga
    20881 agcgaaactg ccctacaaac tggtgttgaa accgctgggc accacgccgg atgaaatcac
    20941 cgctatttgc cgcgacgcga attacgacga tcgttgcgct ggtctggtgg tgtggctgca
    21001 caccttctcc ccggccaaaa tgtggatcaa cggcctgacc atgctcaaca aaccgttgct
    21061 gcaattccac acccagttca acgcggcgct gccgtgggac agtatcgata tggactttat
    21121 gaacctgaac cagactgcac atggcggtcg cgagttcggc ttcattggcg cgcgtatgcg
    21181 tcagcaacat gccgtcgtta ccggtcactg gcaggataaa caagcccatg agcgtatcgg
    21241 ctcctggatg cgtcaggcgg tctctaaaca ggatacccgt catctgaaag tctgccgatt
    21301 tggcgataac atgcgtgaag tggcggtcac cgatggcgat aaagttgccg cacagatcaa
    21361 gttcggtttc tccgtcaata cctgggcggt tggcgatctg gtgcaggtgg tgaactccat
    21421 cagcgacggc gatgttaacg cgctggtcga tgagtacgaa agctgctaca ccatgacgcc
    21481 tgccacacaa atccacggcg aaaaacgaca gaacgtgctg gaagcggcgc gtattgagct
    21541 ggggatgaag cgtttcctgg aacaaggtgg cttccacgcg ttcaccacca cctttgaaga
    21601 tttgcacggt ctgaaacagc ttccaggtct gcccgtacag cgtctgatgc agcagggtta
    21661 cggctttgcg ggcgaaggcg actggaaaac cgccgccctg cttcgcatca tgaaggtgat
    21721 gtcaaccggt ctgcagggcg gcacctcctt tatggaggac tacacctatc acttcgagaa
    21781 aggtaatgac ctggtgctcg gctcccatat gctggaagtc tgcccgtcga ttgccgtaga
    21841 agagaaaccg atcctcgacg ttcagcatct cggtattggt ggtaaggacg atcctgcccg
    21901 actgatcttc aatacccaaa ccggtccagc gattgtcgcc agcctgattg atctcggcga
    21961 tcgttaccgt ctgctggtta actgtatcga cacggtgaaa acaccgcact ccctgccgaa
    22021 actgccggtg gcgaatgcac tgtggaaagc gcaaccggat ctgccaactg cttccgaagc
    22081 gtggatcctc gctggtggcg cgcaccatac cgtcttcagc catgcgctga acctcaacga
    22141 tatgcgccag ttcgccgaga tgcacgacat tgaaatcacg gtgattgata acgacacccg
    22201 cctgccagcg tttaaagacg cactgcgctg gaacgaagtg tattacggat ttcgtcgcta
    22261 agtagccaca tcaggtatgt aacgcctgat gcgacgctga cgcgtcttat ctggcctaca
    22321 cgctgcgatt ttgtaggccg gataagcaaa gcgcatccgg cattcaacgc ctgatgcgac
    22381 gctggcgcgt cttatcaggc ctacgcgctg cgattttgta gaccggataa gcgaagcgca
    22441 tccggcacga aggagtcaac atgttaaaag atctcaaacg ccaggtatta gaagccaacc
    22501 tggcgctgcc aaaacacaac ctagtcacgc tcacatgggg caacgtcagc gccgttgatc
    22561 gcgagcgcgg cgtctttgtg atcaaacctt ccggcgtcga ttacagcgtc atgaccgctg
    22621 acgatatggt cgtggttagc atcgaaaccg gtgaagtggt tgaaggtacg aaaaagccct
    22681 cctccgacac gccaactcac cggctgctct atcaggcatt cccctccatt ggcggcattg
    22741 tgcatacgca ctcgcgccac gccaccatct gggcgcaggc gggtcagtcg attccagcaa
    22801 ccggcaccac ccacgccgac tatttctacg gcaccattcc ctgcacccgc aaaatgaccg
    22861 acgcagaaat caacggcgaa tatgagtggg aaaccggtaa cgtcatcgta gaaacctttg
    22921 aaaaacaggg tatcgatgca gcgcaaatgc ccggcgttct ggtccattcc cacggcccgt
    22981 ttgcatgggg caaaaatgcc gaagatgcgg tgcataacgc catcgtgctg gaagaggtcg
    23041 cttatatggg gatattctgc cgtcagttag cgccgcagtt accggatatg cagcaaacgc
    23101 tgctggataa acactatctg cgtaagcatg gcgcgaaggc atattacggg cagtaatgac
    23161 tgtataaaac cacagccaat caaacgaaac caggctatac tcaagcctgg ttttttgatg
    23221 gattttcagc gtggcgcagg caggttttat cttaacccga cactggcggg acaccccgca
    23281 agggacagaa gtctccttct ggctggcgac ggacaacggg ccgttgcagg ttacgcttgc
    23341 accgcaagag tccgtggcgt ttattcacgc cgatcaggtt ccccgcgctc agcatatttt
    23401 gcagggtgaa caaggctttc gtctgacacc gctggcgtta aaggattttc accgccagcc
    23461 ggtgtatggc ctttactgtc gcgcccatcg ccaattgatg aattacgaaa agcgtctgcg
    23521 tgaaggtggc gttaccgtct acgaggccga tgtgcgcccg ccagaacgct atctgatgga
    23581 gcggtttatc acctcaccgg tgtgggtcga gggtgatatg cacaatggca ctatcgttaa
    23641 tgcccgtctg aaaccgcatc ccgactatcg tccgccgctc aagtgggttt ctatagatat
    23701 tgaaaccacc cgccacggtg agctgtactg catcggcctg gaaggctgcg ggcagcgcat
    23761 cgtttatatg ctggggccgg agaatggcga cgcctcctcg cttgatttcg aactggaata
    23821 cgtcgccagc cgcccgctgc tgctggaaaa actcaacgcc tggtttgcaa accacgatcc
    23881 cgatgtgatc atcggctgga acgtggtgca gttcgatctg cgaatgctgc aaaaacatgc
    23941 cgagcgttac cgtcttccgc tgcgtcttgg gcgcgataat agcgagctgg agtggcgcga
    24001 gcacggcttt aaaaacggcg tcttttttgc ccaggctaaa ggtcggctaa ttatcgacgg
    24061 tatcgaggcg ctgaaatccg cgttctggaa tttctcttca ttctcgctgg aaactgtcgc
    24121 tcaggagcta ttaggcgaag gaaaatctat cgataacccg tgggatcgaa tggacgaaat
    24181 tgaccgccgt ttcgccgaag ataaacctgc gctggcaact tataacctga aagattgcga
    24241 gctggtgacg cagatcttcc acaaaactga aatcatgcca tttttactcg aacgagcaac
    24301 ggtgaacggc ctgccggtgg atcggcacgg cggttcggtg gcagcgtttg gtcatctcta
    24361 ttttccgcga atgcatcgcg ctggttatgt cgcgcctaat ctcggcgaag tgccgccgca
    24421 cgccagccct ggcggctacg tgatggattc acggccaggg ctttatgatt cagtgctggt
    24481 gctggactat aaaagcctgt acccgtcgat catccgcacc tttctgattg atcccgtcgg
    24541 gctggttgaa ggcatggcgc aacctgatcc agagcacagt accgaaggtt ttctcgatgc
    24601 ctggttctca cgggagaaac attgcctgcc ggagattgtg actaacatct ggcacgggcg
    24661 cgatgaagcc aaacgtcagg gtaataagcc gctttcgcag gcgctgaaga tcattatgaa
    24721 tgccttttat ggcgtgctcg gcaccaccgc ctgccgcttc ttcgatccgc gcctggcatc
    24781 gtcgatcacc atgcgtggtc atcagatcat gcggcagacc aaagcattga ttgaagcgca
    24841 gggctacgac attatctacg gagataccga ctcgactttt gtctggctaa aaggtgcaca
    24901 ttcggaagaa gaggcggcga aaatcggtcg tgcactggtg cagcacgtta acgcctggtg
    24961 ggcggaaacg ctgcaaaaac aacggctgac cagcgcatta gaactggagt atgaaactca
    25021 tttctgccgt tttcttatgc caaccattcg cggagctgat accggcagta aaaagcgcta
    25081 cgccgggctg attcaggagg gcgacaagca gcggatggtg tttaaagggc tggaaaccgt
    25141 gcgcaccgac tggacgccgc tggcccagca gtttcagcag gagctgtacc tgcgtatctt
    25201 ccgcaacgag ccatatcagg agtacgtccg ccaaaccatc gacaaactga tggcgggtga
    25261 actggatgcg cggctggttt accgtaaacg ccttcgccgt ccgctgagcg aatatcagcg
    25321 taatgttccg cctcatgtac gcgccgctcg ccttgccgat gaagaaaatc aaaagcgcgg
    25381 tcgccccttg caatatcaga atcgcggcac cattaagtac gtatggacca ccaacggccc
    25441 ggagccgctg gactaccaac gttcaccact ggattacgaa cactatctga cccgccagct
    25501 acaacccgtg gcggagggaa tactcccttt tattgaggat aattttgcta cacttatgac
    25561 cgggcaactt gggctatttt gagcaaaaaa aagagttcgc cagataccat tttgatgcgt
    25621 gacgaatgct ttgccatcca gtaccatagc gccctttcca ttcctggacc tgaataacac
    25681 cactacctca taagcacggt agcgggtggt tattgcctgc aattaaagat atagagccga
    25741 acacatatgc cttttacact tggtcaacgc tggatcagcg atacagaaag cgaattggga
    25801 cttggaaccg ttgtcgcggt ggatgcgcga actgtcactt tacttttccc atctactggt
    25861 gaaaaccgtc tgtacgcacg cagtgattcc cccgtgaccc gcgtgatgtt caaccctggt
    25921 gataccatta ccagccatga cggctggcag atgcaagtcg aagaagtaaa agaagaaaat
    25981 ggcttgctga cctatatcgg tactcgcctg gatactgaag agtccggcgt agccctgcgt
    26041 gaagttttcc ttgatagcaa actggtgttc agcaaaccgc aggaccgtct gtttgccggg
    26101 cagattgacc gtatggaccg ctttgcgctg cgttatcgcg cgcgtaagta ttccagcgaa
    26161 cagttccgta tgccgtacag cggcctgcgc ggtcagcgta ccagcctgat cccgcatcag
    26221 ctcaacatcg ctcatgatgt tggtcgccgc cacgcgccgc gcgtcctgct ggctgacgaa
    26281 gtgggtttag ggaaaaccat tgaagccggg atgatcctgc atcagcaact gctctctggc
    26341 gctgctgaac gtgtgctgat tatcgtcccg gaaaccttac agcatcagtg gctggtagaa
    26401 atgctgcgcc gtttcaacct gcgctttgct ctgtttgatg atgagcgtta tgccgaagct
    26461 cagcacgatg cttacaaccc gtttgacacc gaacagctgg tgatttgctc gctggatttt
    26521 gcccgtcgta gcaaacagcg cttggaacat ctctgtgaag ccgaatggga cctgctggtg
    26581 gtcgatgaag cgcatcacct ggtgtggagc gaagatgcgc caagccgtga atatcaggcc
    26641 attgaacaac tggcagagca cgtgccgggc gttctgctgc tgaccgcgac cccggaacag
    26701 ctggggatgg aaagccactt cgcccgtctg cgtctgctgg acccgaaccg tttccacgat
    26761 tttgcgcagt tcgttgaaga gcagaaaaat tatcgtccgg ttgcggacgc cgttgccatg
    26821 ctgctggcag gtaacaaact gagcaatgac gaactgaaca tgctcggcga gatgatcggc
    26881 gagcaggata tcgagccgct gttgcaggca gcaaacagcg acagcgaaga tgcccagagc
    26941 gcccgtcagg agctggtttc gatgctgatg gatcgccacg gcaccagccg cgtgctgttc
    27001 cgtaacacgc gtaacggtgt gaaaggattc ccgaaacgcg agctgcacac cattaagctg
    27061 ccgctaccga cgcagtatca gacggctatt aaagtctccg gcattatggg cgcacgtaaa
    27121 agttcggaag atcgtgctcg cgatatgctc tacccggagc gtatttatca ggaatttgaa
    27181 ggtgataacg ccacctggtg gaacttcgat ccgcgcgttg agtggctgat gggctacctg
    27241 accagccatc gctctcagaa agtgctggtg atctgcgcca aagctgccac tgcgctgcaa
    27301 ctggagcagg tactgcgcga acgtgaaggt attcgcgctg cggtgttcca cgaaggtatg
    27361 tcgattatcg aacgtgaccg cgctgccgcc tggtttgccg aagaagacac cggcgcacag
    27421 gtactgctgt gctcagaaat cggttctgaa ggacgtaact ttcagttcgc cagccacatg
    27481 gtgatgtttg acctgccatt caacccggat ctactggagc agcgtattgg tcgtctggat
    27541 cgtatcggcc aggcgcacga tattcagatc catgtgcctt atctggagaa aaccgctcag
    27601 tcggtgctgg tgcgctggta tcacgaaggt ctggatgcat ttgagcacac ctgcccgacc
    27661 ggacgcacta tttacgatag cgtatacaac gatctgatta actatctggc ttcaccggat
    27721 caaaccgaag gctttgacga tctgatcaaa agctgccgcg agcaacatga agcgctgaaa
    27781 gcacagctgg aacagggtcg tgaccgcctg ctggaaatcc actccaacgg tggcgaaaaa
    27841 gcccaggaac tggcagaaag cattgaagag caggatgacg ataccaacct gatcgccttc
    27901 gccatgaacc tgttcgatat tatcggtatc aatcaggacg atcgcggcga caacatgatc
    27961 gtgctgacgc cgtccgatca tatgctggtg ccggacttcc ctggcctgtc ggaagatggc
    28021 atcaccatca cctttgatcg tgaagtggcg ctggcgcgtg aagatgcaca gtttattacc
    28081 tgggagcatc cgctgatccg caacggtctg gatctgatcc tttctggcga taccggtagc
    28141 agcacgattt cactgttaaa aaacaaagcg ttgccggtag gtacgctgtt ggttgaactg
    28201 atttatgtgg ttgaagccca ggctccgaag cagttgcagc tcaaccgctt cctgccaccg
    28261 acgccggtac gtatgctgct ggataaaaac ggcaacaacc tggcggcgca ggtagagttt
    28321 gaaaccttta accgccagct taacgcggtt aaccgtcaca ccggcagcaa actggttaac
    28381 gccgtgcagc aggatgttca cactatcctt caactgggtg aagcgcagat cgagaaatct
    28441 gcccgtgcat tgattgatgc agcgcgtaac gaagccgacg aaaaactgtc tgccgagctg
    28501 tctcgtctgg aagctctgcg tgcagtgaac ccgaacattc gtgacgacga actgaccgcc
    28561 attgagagca accgtcagca ggtaatggaa agcctggatc aggcaggttg gcgtctggat
    28621 gccctgcgtt tgatcgttgt aacgcatcag taacggagcc gaaaatgggg atggaaaact
    28681 acaatccacc gcaggaaccc tggttggtta tcctgtatca ggatgaccat attatggtgg
    28741 tcaacaagcc gagcggtttg ttgtcagtgc cgggtcgtct ggaagagcac aaagacagcg
    28801 tgatgacgcg cattcagcgt gattatccgc aggcagaatc ggtgcatcgt ctggatatgg
    28861 ctaccagcgg cgtgattgta gtggcgctga ccaaagccgc ggagcgggag ttaaaacgcc
    28921 agttccgcga gcgcgagccg aaaaagcagt atgtggcccg cgtctggggg catccatccc
    28981 ccgcagaagg tctggtggat ctgccgctga tttgcgactg gccaaaccgc ccgaaacaga
    29041 aagtctgtta cgaaacgggt aaacctgcgc agacggaata tgaagtggtg gagtatgcgg
    29101 cggataacac ggcaagagtg gtgttaaaac cgattaccgg gcgttcgcat caattgcgtg
    29161 tgcatatgct ggcgctgggg catccgattc tcggcgatcg tttttatgca tcaccagaag
    29221 cgagagcgat ggcaccgcgt ttgttgctac atgcagagat gttgacgatt acccatccgg
    29281 cgtatggcaa tagtatgacg tttaaagcgc cagcggattt ttaatcgtca cgctggcgca
    29341 gttcggtagg cggataaggc gttcacgccg catccggcaa ttgtgcgccg attgcctgat
    29401 gcgacgcggg ccgcgtctta tcaggcctac agtttaaaac ttatttaaac cctttctgct
    29461 gctttatcag ctcatatgcc tgctgaattt cctgcgcttt ctgcttcgcc atctccatca
    29521 tctcaggcgg caaacctttc gccaccagct tatcgggatg gtgttcactc atcagcttac
    29581 ggtaggcacg tttgatggtg gtcgcatcat ccgtcggctt cacgcccagc acattacagg
    29641 catcttccag cgtcgggcca cgctgcgctt gctgccagtt accaccgcca gattgctgct
    29701 gataaccgcc gccaaactgt gcaccgccct gcatcatgcg caaaaactgg tcaaactggg
    29761 cgcgggagat ccctaattct tcagcaatga catacagcac cgcccgttca ttcgggtgca
    29821 gtgaaccatc agcaaacgcc gcctgaatct ggatctccag aaacatacga attaagtcaa
    29881 aacgaccaaa gcagacactg cgaaactggc gcatcttttc gcgcagcggg taattatctg
    29941 attttcccac ccggaacgcg ttttgcgccg cagtacggga agcgccatga aggttcattc
    30001 ggtccatcaa ctggctggcg atatgaatat cagcctccgt gacgcgacct ttggatttgg
    30061 ttaaatgccc catcacttca aaagtggtgg caaaaaacag cgcctgacgc tcacgctggt
    30121 tggcgaacca cgccatttta cggctacggg ctttatcaaa catatggcca attaacaggc
    30181 ctaacactac gccccaaaag ccgccgccca tcagtaaggc cacggccacg ccaatgattt
    30241 ttccccaata ctgcatatat tccccaaatc gacacacgga tatcagggct atctcccaca
    30301 atataaaggt gcttttaccg ttttccggct gcggtcaatt gtgggacatc gcctataatt
    30361 tgcattatca tacctgtcat tcaatgccgt gcctaacacc actgacgcta ttcggacagg
    30421 attaacacta gcgtagagat gacgagtacg ttagtctctg accgtttgtc acgcgcaacg
    30481 ttaccgatga tggaacaata aaatcaacgt atgaaaaaac gtatccccac tctcctggcc
    30541 accatgattg ccaccgccct ttatagtcaa cagggactgg cagccgacct cgcctcacag
    30601 tgcatgttgg gcgtgccaag ctatgaccgt cctctggtac agggcgatac caatgactta
    30661 cccgtgacta tcaatgctga ccacgcgaaa ggggactacc cggatgacgc cgtgtttact
    30721 ggcagcgtgg atatcatgca gggtaacagc cgtctgcagg ccgacgaagt gcagctccat
    30781 caaaaagagg caccaggaca accggagccg gtacgtaccg ttgatgcgct cggtaatgtc
    30841 cattacgacg ataaccaggt gatcctcaaa gggccgaaag gctgggcgaa tctgaacacc
    30901 aaagatacca acgtctggga aggtgattac cagatggtgg gtcgccaggg tcgcggtaaa
    30961 gcggacctga tgaaacaacg tggcgaaaac cgctatacca ttctggataa cggtagcttt
    31021 acctcctgtc tgccgggttc tgacacctgg agcgtggtag gtagcgaaat tattcatgac
    31081 cgcgaagaac aagttgcgga gatctggaac gcccgcttta aggtgggtcc ggtaccgatc
    31141 ttttatagcc cctatttgca gttgccggtg ggtgacaaac gtcgctctgg tttcttgatc
    31201 ccgaacgcca agtacaccac caccaactac tttgagttct acctgccata ttactggaac
    31261 atcgcgccaa atatggatgc caccatcacg ccgcattata tgcatcgtcg tggcaacatc
    31321 atgtgggaga acgaattccg ctacctctcc caggcgggcg ctggcttgat ggaactggac
    31381 tatctgcctt cagataaagt ctatgaagat gaacacccga acgatgacag ttcacgtcgt
    31441 tggttattct actggaacca ctccggggtc atggatcagg tgtggcgttt caacgtcgac
    31501 tacaccaagg tcagcgatcc tagctacttc aatgatttcg ataacaagta cggttccagt
    31561 actgacggct acgcaacgca aaaattcagc gttggctatg cggtgcaaaa cttcaatgcc
    31621 accgtttcaa ccaagcagtt ccaggttttc agcgaacaga acaccagtag ctactcggca
    31681 gagccgcagt tagacgttaa ttactaccag aatgatgttg gtccgtttga tacgcgtatt
    31741 tacggccagg cagtgcactt tgttaacacc agagacgaca tgcctgaagc aacccgtgtt
    31801 cacctggaac cgaccatcaa tttgccgctc tctaataact ggggcagcat caataccgaa
    31861 gcgaagtttc tggcaaccca ttatcagcaa accaatcttg actggtataa ctccagaaac
    31921 acgaccaagc tggacgaatc cgttaaccgc gtaatgccgc aattcaaagt tgacggcaaa
    31981 atggtctttg aacgcgatat ggaaatgctg gctccgggtt atacccaaac gctggaaccg
    32041 cgcgcgcagt atttgtacgt gccgtatcgc gatcagagcg acatctataa ctacgactcg
    32101 tctctgctgc aatctgacta ctctggcctg ttccgggacc ggacttacgg cggtcttgac
    32161 cgtattgcct ccgctaacca ggtgacgacc ggtgtcacat ctcgcatata tgatgatgct
    32221 gccgttgaac gttttaatat ttccgttggt caaatctact atttcacgga gtctcgcact
    32281 ggcgatgaca acataacatg ggagaatgac gacaaaacgg gctcactggt gtgggcaggc
    32341 gacacctact ggcgtatctc cgagcgttgg ggattgcgtg gcgggattca gtacgataca
    32401 cgtctggata acgtagcgac cagtaactcc agcattgaat accgtcggga tgaagaccgt
    32461 ctggtacagc tgaattacca ttacgccagc ccggaatata ttcaggctac gctgcctaag
    32521 tactattcca ctgctgagca atataagaat ggtatttcgc aggtaggtgc tgtcgccagc
    32581 aggccaattg ccgatcgttg gtccattgtt ggggcctact actacgacac caatgcgaac
    32641 aagcaagccg actctatgtt aggtgtgcaa tacagctcct gctgctatgc aattcgcgtc
    32701 ggttacgagc ggaagctgaa cggttgggat aacgataaac aacatgcggt atatgacaac
    32761 gcaatcggct ttaacatcga acttcgcggc ctgagctcca actacggtct gggtacgcaa
    32821 gagatgctgc gttcgaacat tctgccgtat caaaacactt tgtgatttgt tgatttacca
    32881 cgtaatccgc agtgcggtta attgaaatgg aaaaagtatg aagaactgga aaacgctgct
    32941 tctcggtatc gccatgatcg cgaataccag tttcgctgcc ccccaggtag tcgataaagt
    33001 cgcagccgtc gtcaataacg gcgtcgtgct ggaaagcgac gttgatggat taatgcagtc
    33061 ggtaaaactg aacgctgcgc aggcaaggca gcagcttcct gatgacgcga cgctgcgcca
    33121 ccaaatcatg gaacgtttga tcatggatca aatcatcctg cagatggggc agaaaatggg
    33181 agtgaaaatc tccgatgagc agctggatca ggcgattgct aacatcgcga aacagaacaa
    33241 catgacgctg gatcagatgc gcagccgtct ggcttacgat ggtctgaact acaacactta
    33301 tcgtaaccag atccgcaaag agatgattat ctctgaagtg cgtaacaacg aggtgcgtcg
    33361 tcgcatcacc atcctgccac aggaagtcga atccctggcg cagcaggtgg gtaaccaaaa
    33421 cgacgccagc actgagctga acctgagcca catcctgatc ccgctgccgg aaaacccgac
    33481 ctctgatcag gtgaacgaag cggaaagcca ggcgcgcgcc attgtcgatc aggcgcgtaa
    33541 cggcgctgat ttcggtaagc tggcgattgc tcattctgcc gaccagcagg cgctgaacgg
    33601 cggccagatg gggtggggcc gtattcagga gttgccgggg atcttcgccc aggcattaag
    33661 caccgcgaag aaaggcgaca ttgttggccc gattcgttcc ggcgttggct tccatattct
    33721 gaaagttaac gacctgcgcg gcgaaagcaa aaatatctcg gtgaccgaag ttcatgctcg
    33781 ccatattctg ctgaaaccgt cgccgatcat gactgacgaa caggcccgcg tgaaactgga
    33841 gcaaattgct gctgatatca agagtggtaa aacgactttt gctgccgcag cgaaagagtt
    33901 ctcgcaggat ccaggttctg ctaaccaggg cggtgatctc ggctgggcta caccagatat
    33961 tttcgatcca gccttccgtg acgccctgac ccgcctgaac aaaggtcaaa tgagtgcacc
    34021 ggttcactct tcattcggct ggcatttaat cgaactgctg gatacccgta atgtcgataa
    34081 aaccgacgct gcgcagaaag atcgtgcata ccgcatgctg atgaaccgta agttctcgga
    34141 agaagcagca agctggatgc aggaacaacg tgccagcgcc tacgttaaaa tcctgagcaa
    34201 ctaatggtta aaacccaacg tgttgtgatc actcccggcg agcccgccgg gattggcccg
    34261 gacttagttg tccagcttgc acagcgtgag tggccggtcg aactggttgt ttgtgccgat
    34321 gccactctcc ttaccgaccg ggcagcgatg ctcggtttgc cgctcaccct ccgcccttat
    34381 tcccccaact cccctgcaca accgcaaact acgggcacat taacgctact tcctgtcgcg
    34441 ctacgtgaat ctgtcactgc ggggcagtta gcgattgaaa atgggcatta cgtggtggag
    34501 acgctggcgc gagcgtgtga tggctgtctg aacggtgaat ttgctgcgct gatcacaggc
    34561 cccgtgcata aaggcgtcat taacgacgca agcattccgt ttaccggtca taccgagttt
    34621 ttcgaagagc gttcgcaggc gaaaaaagtg gtgatgatgc tggcgaccga agaacttcgc
    34681 gtggcgctgg caacgacgca tttaccgctg cgcgatatcg cagatgctat cacccctgca
    34741 cttttgcacg aagtgattgc tattttgcat cacgatttgc gtacaaaatt tggtattgcc
    34801 gaaccgcgca ttctggtctg cgggctgaat ccgcacgcgg gcgaaggcgg tcatatgggt
    34861 acggaagaga tagacaccat tattccggtg ctcgacgagc tgcgggcgca ggggatgaaa
    34921 ctcaacgggc cgctgcctgc cgataccctg tttcagccga aatatctcga taacgccgac
    34981 gccgtgctgg cgatgtacca cgatcagggt cttcccgtgc taaaatacca gggcttcggg
    35041 cgcggtgtga acattacgct gggcctgccc tttattcgca catcagtgga ccacggcacc
    35101 gcgcttgaac tggcgggacg tggcgaagcc gatgtcggca gttttattac ggcgcttaat
    35161 ctcgccatca aaatgattgt taacacccaa tgaataatcg agtccaccag ggccacttag
    35221 cccgtaaacg cttcgggcaa aactttctca acgatcagtt cgtgatcgac agcattgttt
    35281 ctgccattaa cccgcaaaag ggccaggcaa tggtcgaaat cggccccggt ctggcggcat
    35341 tgaccgagcc ggtcggcgaa cgtctggacc agctgacggt catcgaactt gaccgcgatc
    35401 tggcggcacg tctgcaaacg catccattct taggcccgaa actgacgatt tatcagcagg
    35461 atgcgatgac ctttaacttt ggcgaactgg ccgagaaaat gggtcagccg ctgcgtgttt
    35521 tcggcaacct gccttataac atctccacgc cgttgatgtt ccatctgttt agctatactg
    35581 atgccattgc cgacatgcac tttatgttgc aaaaagaggt ggtgaatcgt ctggttgcag
    35641 gaccgaacag caaagcgtat ggtcgattaa gcgtcatggc gcaatactat tgcaacgtga
    35701 tcccggtact ggaagttccg ccgtcagcct ttacaccacc acccaaagtg gattccgccg
    35761 tcgtgcgcct ggtccctcat gcaacgatgc ctcacccggt taaagatgtt cgcgtgttga
    35821 gccgcatcac caccgaagcc tttaaccagc gtcgtaaaac cattcgtaac agcctcggca
    35881 acctgtttag cgtcgaggtg ttaaccggaa tggggatcga cccggcgatg cgagcggaaa
    35941 atatctctgt cgcgcaatat tgccagatgg cgaactatct ggcggagaac gcgcctttgc
    36001 aggagagtta acgatgatca attcgccccg agtgtgtatt caggttcaaa gcgtctacat
    36061 tgaggcacaa tcttcacctg ataatgaacg ttacgttttt gcttataccg taaccatacg
    36121 caatctgggg cgagcgccag tgcagttgtt ggggcgttac tggctgatca ccaatggcaa
    36181 tggccgtgaa accgaagtcc agggcgaagg agtggttggc gtccagccac ttatcgcgcc
    36241 tggcgaagag taccagtaca ccagcggtac aatcattgaa accccgctgg gcaccatgca
    36301 gggtcactac gaaatgatcg atgaaaatgg cgtccctttc agcatcgaca ttcccgtatt
    36361 ccgactcgcc gttcccacac tcattcatta aaagaatatg gcgacatacc ttattggcga
    36421 cgttcatggt tgttacgatg aactgatcgc attgctgcat aaagtagaat ttacccccgg
    36481 gaaagatacc ctctggctga cgggcgatct ggtcgcgcgc ggcccgggtt cgctggatgt
    36541 tctgcgctat gtgaaatcct taggcgacag cgtacgtctg gtgctgggca atcacgatct
    36601 gcatctgctg gcggtatttg ccgggatcag ccgcaataaa ccgaaagatc gcctgacacc
    36661 gctgctggaa gcgccggatg ccgacgagct gcttaactgg ctgcgtcgcc agcctctgct
    36721 gcaaatcgac gaagagaaaa agctggtgat ggcccacgca gggatcacgc cgcagtggga
    36781 tctgcagacc gccaaagagt gcgcgcgcga tgtagaagcg gtgctgtcga gtgactccta
    36841 tcccttcttt cttgacgcta tgtacggcga tatgccaaat aactggtcac cggaattgcg
    36901 ggggctggga agactgcgtt ttatcaccaa cgcctttacc cgtatgcgtt tttgcttccc
    36961 gaacggtcaa ctggatatgt acagcaaaga atcgccggaa gaagcccctg ccccactgaa
    37021 accgtggttt gcgattcctg gccctgtcgc tgaagaatac agcatcgcct ttggtcactg
    37081 ggcatcgctg gaaggcaaag gtacgccgga aggtatttac gcgctggata ccggctgctg
    37141 ctggggtggg tcattaacct gcctgcgctg ggaagataaa cagtattttg tccagccgtc
    37201 gaaccggcat aaggatttgg gcgaagcggc ggcgtcttaa acacagcctg atacaggaag
    37261 gccggataag acgcgaccgg cgtcgcatcc ggcactagcc gtaaattcta tacaaactta
    37321 ccgccgctcc agaatctcaa agcaatagct gtgagagttc tgcgcatcgg cgtcgtggaa
    37381 ttcgctgaat accgattccc agtcatccgg ctcgtaatcc gggaaatggg tgtcgccttc
    37441 cacttctgcg tcgatatgcg tcagatacag tttttgcgct tttggcagga actgctcata
    37501 aacgcgaccg ccgccaatca ccatgatttc tggtacgtca ccacacgccg caatggcttc
    37561 atccaccgat ttcacccacg ttacgcgatc gtccgtaccc ggttgactgc tgaggataat
    37621 atttttgcgt cctggcaacg gacgaccgat tgattcccaa gtatggcggc ccataatcac
    37681 gggtttattt aaggtgttgc gtttaaacca ggcgagatcg gcaggcaggt tccacggcat
    37741 ggcgttttcc atgccgataa cgcgatctac cgctaacgcc gcaatcagac tgatcattga
    37801 gatttcccga taaaaaaatt gtcgccacta tacgtaaagc gtaaaccgtc gtcgactggt
    37861 gcgaggatga tgttgaggaa aattttatat tctgctggcg agtccacgct ctccccctgg
    37921 actcgccgca ttacaatgaa acaaaaacaa acagttagct gtaaagtgtg atttacgtca
    37981 ctctttatta agatgagggt ttcgtttccg gttcatccgc catgttgccg gtatgtttac
    38041 cttcttctgt tccctgccag ccatgtcgtt gaattaatga cagatgttcg cggtcctcgg
    38101 tgatgatctc acttaacatc gcgctggtgc gtttatagac cgccgcgcgg gctttggtgt
    38161 cattctcaac cattgccatc tcttccacca tctgaatatt aaagcggcgg aacacatcgg
    38221 cacgttctcg cgcttcatac ggccccagac ctaaactttc cagcgccaga cgcccggttt
    38281 tcagcgcacc ttcaaaggtt tcacgctccg gcttttcaac gcctgcctga cgcaaacgaa
    38341 tgtagtgatc gacatcgcgg gcgcgggcaa taatctgcaa atgcgggaaa tgttctttca
    38401 ccatctccgt cagttgcagg ttggtttgcg gatcgtcaat agcgttaatc agcacttccg
    38461 ctttcgccgc tccggcagat tccagtaaat ccatccgcgt ggcatcgcca taaaacactt
    38521 tcataccaaa tttacgcagc gtttcgatat ggtccggatc gtgatcgagc accaccattt
    38581 tcaccccgct ggagagcagt aaacgtccgg taatctgtcc aaaacgaccg aatccggcga
    38641 taatcacgcg cggctgttct tcatcgatct catcggcttc acgcgcctcc tccgtagaag
    38701 attgctcaag gcgattgagg atcaccagca gaatcggcgt tgctgccatc gacagcgcca
    38761 ccgccagggt cagcgatttc gcccactccg gctccagcac attcgccatc tgcgccgcac
    38821 caaataccac aaaggcaaac tcactgccct gccctaacaa caccgcaaac cagcgacgct
    38881 gtttatttgg cacctgcaac ggtcgggcaa tcagccacag catggcgatt ttgatgatga
    38941 ggaaaccgag cagcaaaatg acaatgcgca atgggttttc aatcagcgtg ccaaagtcta
    39001 tcgacatgcc aacaccgatg aaaaacagcc ccaacagcaa acctttaaac ggttcgatat
    39061 cgctttccaa cgcatgacgg tattccgagc ttgccagcag tacgcccgcc agaaacgcgc
    39121 ccatcgccat cgacaagccg acctcttcca gcagcaaacc aaagccaaac acaaggaata
    39181 acgccacggc actaaacact tcccgcaagc cagagcgggc aacaaaacgc agcgccggac
    39241 gcgtaacata gcgccccagc aataccacca gtaccagcgc acccgccact tttaacgccg
    39301 agagagcaaa tgcgcccatc gtcgtcgagg cactgctcgc cgccagtagc gggatcatcg
    39361 ccaccagcgg gattgccgcg atatcctgga acagcagcac tgcaaaggca ctgcggccca
    39421 tttgcgtcac catcagattg cgttcattca tcgcctgcat ggcaatcgcc gttgatgaga
    39481 gcgccagcgt cataccgatc aattccgcga cctgccagcg cagcccaagt aacatacaga
    39541 acagccccag caggccaccg caaatcacca tctgcaatgc gccaccgccg aacactgccg
    39601 cacgcagctt ccacagcctt tgtggatcga gttcgaggcc gataataaac agcatcagca
    39661 ccaccccaat ctcggcaaag tgcagaatag attcggcatc ggtcaccagt cgcagccccc
    39721 acgggccaat aatgcagccg gcgatcaggt agccaagtac cgatcccaga ccaagacgta
    39781 ccgcaatggg tacaatcagc gccgccgatc cgaggtagat cagcgcctga accagcgtat
    39841 gactatccat gatgggcctc ctgccattcc agcagacgtt gcttatagtg gcgcgcctgc
    39901 ccttcgaggg tttcgtcgtc acaaataaag gtgcagtgca tggcaaacgg tggcagccag
    39961 ttcagcccgc agtagattgc cgtcgcctgt agcggctgcg acagcacatc aaagcccgga
    40021 tgcgcaccaa tttcaaaatg gctttccccg ccgccggtcg tcaccgccca cagcaaatgt
    40081 ttgccatgca gcgccgtgcc gccgtgaccg taagcccagc cgtgcgacaa aactttatcg
    40141 atccaaagtt taagaagcgg aggaatgctg taccactgca tcgggtgctg ccagacgatc
    40201 agatcggcgc gagagagcgc ctcctgctcg gcggcaatat cgatattgaa gtcaggatag
    40261 agttgataaa gagagcgaat ttcgacgcct tccagcgtcc ttgcctgttc aagcatccgt
    40321 ttattcgcat gggaatgatg cggatacgga tgcgcataaa tgatgaggat catagttatt
    40381 agcccgcgtg tttacgcctt tgttttccca caaagtgtag tcatatattc acgcggctta
    40441 tagtcaataa tattgattca gaaaagagag aaatctgaac aaataatccc attctctgcc
    40501 cgcggatcgt catattgctg gtctgcgcca gtgacattcc tcgagtctcc ggggcaaacg
    40561 ctacggaaat caacaagcca aacagcgaga tacccgcccc cattagcatc gtgttactga
    40621 taccgtaatt attgataaat atcggcagtg cccaggtcga aacaatggtg ccaatacgac
    40681 ttaaggacat aatcacgccc acggcagagg cgcggatatc cgtcgggaag agttcattag
    40741 gatagagcca ctgcaaatta cccggcccgc cagagaaaaa ggcatacacc gcaaaggcca
    40801 tcactaccag ccagatcccc atatccggga ttagccccaa aaccgccagc gccagcgtca
    40861 tcatggcaaa gctgccaatc aacaatggac gccgtccggc agtgtttagc cacagcatcg
    40921 gcggaataca gccgagcata aagaacaggc taatcaccac attccctagt gccgcgtttt
    40981 tgccaacccc caatcccaac gaaccaacga tttgcgggcc aaaggtgtaa atggcgaaca
    41041 ttgggatcac ctggcaggtc cagatggcgg caacaaacag aacaaaagga aaatggcggc
    41101 gattaaacag atcgcgaaaa cgggtttgct gcggctgctc ttcatcgaaa gccaccggtt
    41161 cgccaaacag ttttatcatc atctcttcgc actcttttac tcgccctttg cgtaataacc
    41221 agcggggaga ttctggcagt tcgaatcgac caatcaaaat caacaaacaa gggatcgccg
    41281 cgctacccag catccagcgc caaccacctt ccacatcata aagccagtag ccgaccagat
    41341 cggcgcaggt cgcgccgaca taccacatcg cggcaataaa gctgatggaa aacgcccgct
    41401 gacgggtact ggagaactcg gtgatcatcg aagtggcgat gggataatct gcaccgatga
    41461 caatgccgat gagtacccgc atcaccaaca gttcgacggg ggatgaaaca cacatcgtcg
    41521 ccaccgatat cacgccgatg gcgatgatat caatgaggaa cattttgcgc cgtccgactt
    41581 tatcggagat atagccaaac agcgatgtgc caacgaacag cccggcgagc gttcccgcgc
    41641 ccagcaagcc aatccagtca gcgtccagtt tcagcgccgg agtcagttgc tccagcgcca
    41701 cgccaatcat taccagtaca taaccatcca gaaacggacc accgcttccc cacagcaaaa
    41761 tgcggcggtg aatagaggag aatttgagat cgtcaaagtt tctggacggt tgcatgggtt
    41821 attcctgttt ttttgcaatg agagtcagta cggcgttacg tatcaaacca acatcagccg
    41881 taacggaact ccacaccaaa ggtgccgcgc gggtattccc actgttccag cgccgacccc
    41941 agccccagaa tgcgacaggt gccgcactcc agacatccgg cgtaatcgaa gcgcacactg
    42001 ccgtcatcct gcttcttgta cagacctgcg gggcacgctt tcaccagcag ctccagcacc
    42061 tgtttatcag catcggcctt cacaacaatg tgcggatgct cttcatcgac attgaattta
    42121 ttgacgccca gtttgacgtc cacattgacg ggagaagtca taaaacggtc actcctttca
    42181 tgccatcctt gatcagattg atgaagccca ctttcttgcc gtggcggagg attttcttgc
    42241 gcatcagttc cggcgcactg ccgtcaatgg tgaacaggtc acgcgccacg cccaccgcca
    42301 gttccgggtt gccgctaaac atgcgtgggt tatcaaggaa cgccggtagt ttctggtaca
    42361 tgcgcatatc gcgcagcggg ccactctcca gatgctgacg atattccgcg agtttttgct
    42421 tactgaaatc gtcgcttttc atcgctgaaa gcacggtttt tgctgcagct tccccggcgg
    42481 caatcgccag atccataccg cgaatggtaa aaccgaggtt catacacatt ccggcggcat
    42541 caccggcaat caatacgccg tcaccgacca gctccggcag catgttgatg cctgcttccg
    42601 gcactacgtg agcggaatat tccaccagct tgccgcccgc gatcagcggt gcaacggccg
    42661 gatgctgttt gaaatcttcc agcatttgcg gcaccgattt tttcgcgtca tgcagatgat
    42721 gcagaccaca aaccagcccc agcgacaggg tattttcatt ggtataaagg aagccgccgc
    42781 ccatcaggcc atcggtgggt gatcccgcaa acagacaagc cgcgccctga ttaccctgca
    42841 actgaaaacg gtcttcgatt accgacttcg gtaactcgat cagttccttc acgccaaccg
    42901 ccacatccgt cggtttaacg cgttttgcca tccccaactt ttcggcaagg atggagttca
    42961 ccccatcagc aaggagcacc gttttcgctt caatcacatc gccatcggct tctacaccga
    43021 cgactttgcc atcgcgctgt acgaggttat cgacgcggat cccggtaatt aactgcgcgc
    43081 ccgcttcttc ggcctgctcc atcaaccagg catcaaattt actgcgcaaa acggagtaag
    43141 aacgctggga tggcgaggtt tcgtcaccgt tgcagtagtc catagtcatc gctgactttt
    43201 ccgtcataaa cgcgagtttt tcatgggtga tcaggcgttc tacgggggcg gaatcggcga
    43261 aacctggaat aatgtgttcc agactgtggg catagagacg cccgccagta acgttcttgg
    43321 cacctgcaga attgccacgc tcgataacta acacttgcgc cccttcgcgg gcgagcacca
    43381 gtgcggcaac cgaaccggca agccctgcac cgacgatgat ggcgtcaaag atatcttcgg
    43441 acataagagc tccctgtcag cggcagagcc gtaatgcagc cctgccagag tggatcaacg
    43501 cgctaaagct gcggtcagtg ccggaaggat cttcacggca tcgccaacaa tgccgtaatc
    43561 cgcgtactgg aagatcggcg catttttatc tttattgatg gcgaaaatgg tttgcgacgc
    43621 gttagcgcca accatgtgct ggatctaccc ggagatcccc accgccaggt acagttcagg
    43681 tttcagcatc aggttggaga taccgacata gcgttcgtgc tccatccatt tttcgttttc
    43741 tgccaccgga cgagaacagg ccaactccgc acctatcgcc ttgcaaagct gttctgccag
    43801 cgcaatgttc tctttgctgc caatgccgcg accgacgctg accaccagac gggctttgtc
    43861 gagatcgacg ctgttgctct ggcgcgcctg ggttgccgtg cgggtaatcg ccacagccgg
    43921 agcctgccac tccacggtgt gcgtttcgcc agtgcgtgac gcgtctggct gagccgcatc
    43981 gaacgtgccg ctgctgatgg tcagtaccgc atacggcgtg gcaatgcgtt cttcgccaat
    44041 cgccagaccg ccgtaaacca tgtgtttcac tgttgcttta ccgtcctgta cgctgacggt
    44101 gctggcatcg ttagacaccg ccgctttaag gcgataaccc agttttgccg ccagtaattt
    44161 gccgcgacgg gtgtttggca acagcaccag accgtcagcg ccgtgctggc gaatagtgtc
    44221 agccatgaca ccggcgtaat cttcgatcat ccgatcgtcc ggtttgccgt ttaatttcca
    44281 gacatgatta gcgccgagct ggattgcctg tgcgccgtcg gcatcattga ggacaaaggc
    44341 attgatttga ttagctaaag cctgcgcacc gttcatcagt tccggcagac gagagggggt
    44401 atcgctgaat acccagactt gagaaaacgt gttcatagca tcccctgtaa ttaaatgact
    44461 ttgcgaagat tttcagcaaa tgcggcgatc tgttcttcgc cgtcgccttc aatcacgatg
    44521 cgctgacgct cgcgctgttt cggcgcggca acctgttgtt cagaccaggc gtccacggcg
    44581 ttaaaaccaa tatccgccgc cgaccatacc tggactggct ttttcgccgc gccgagaatg
    44641 gctttcatcg aaggaatttg tggggagttg atatcagtgg aaacagcaac aaccgcaggc
    44701 agcggaatgc ttaaggtttc ggtttcatct tccagttcgc gctcaacggt gagggtatct
    44761 gccgtcaggg agataatttt gctgacgccg ttaactgccg gaatattgag gatttcgccc
    44821 accagcagac caacctgctg ggcataaagg tcggaagaac catcgccaca gaggatcaga
    44881 tcaaagcctg ctttctgggc ggctgcagcc agtgcgctcg ccgtttgttg cggcagtgcc
    44941 tgctcgaact ggtcatcaat caccacaatc agttcatccg ggccgcgcga tagcacatct
    45001 ttacgccctt tggcgttggt cagggcttta ccgcccacac ttaaggctgt cacctgcgcc
    45061 tctgctgcct gttgctttag ctggcaagcc gcttcaatag cgttgagatc gtattggctt
    45121 attttggcat cggctttgct gaagtctaat gaaccatcag cattattgac cgcaatatcc
    45181 tgttcatcag gcacgcactt atagcaagta ataatcttca ttgcatctcc agaaatcatg
    45241 aaggaaacgt cgttcatatc ggtggtgtta attacagata tcacgccctt taatttggaa
    45301 ataaaacttt aatcaccaat attgaaaatg tcacacgcag ataaatccaa ctttcaatat
    45361 tgttaagttc ctcaccaata ttgaaaacat ggcgtagcaa aaagaaattt ttaatattgt
    45421 tttatagatc accaatattg aaagtcagat gctatttttt tacccacaaa agaatataac
    45481 aaactgatta ataaccaaaa ttaacgaaaa acgccctcta ttcactcgaa acaaaaatgt
    45541 gacaccaatc acagaataca gcttattgaa tacccattat gagttagcca ttaacgcgtc
    45601 cacgaggtta ataataatta tattaaatgt taacaaaaat aaaacaaacg ggaacgcaaa
    45661 ataaatattc gttttcacag tggaattaac tcatgaagaa tgaaaagaga aaaacgggaa
    45721 tagaaccgaa ggttttcttt ccgccattaa taatcgtcgg catactttgt tggcttacag
    45781 tcagagatct ggatgcggcg aatgtcgtta ttaatgctgt attcagttac gtcaccaatg
    45841 tatggggatg ggcatttgaa tggtatatgg tggtgatgct tttcggttgg ttctggctgg
    45901 tgtttggccc gtatgccaaa aagcgtttag gtaacgaacc gccagaattt agcaccgcca
    45961 gttggatctt tatgatgttc gcctcctgta cgtctgctgc cgtactgttc tggggatcga
    46021 ttgagatcta ctactacatc tccaccccgc cgtttggctt agaaccgaac tcgacaggag
    46081 cgaaagagtt ggggctggct tacagcttgt tccactgggg acctctgccg tgggccactt
    46141 acagcttcct ttcagtcgcc ttcgcttact tcttctttgt ccgcaaaatg gaagtgattc
    46201 gccccagctc gacactggtg ccgctggtag gtgaaaaaca cgccaaaggg ttgttcggta
    46261 ccatcgtcga caacttctat ctcgtcgcct tgatcttcgc gatgggtacc agtctgggcc
    46321 ttgccacgcc gctggtgacc gagtgtatgc aatggttgtt tggcattccg cataccctgc
    46381 aactggacgc tatcatcatt acctgctgga ttatcctcaa cgccatttgc gtcgcttgcg
    46441 gtctgcaaaa aggggtacgt atcgccagtg acgtgcgtag ttacctgagc ttcctgatgc
    46501 tgggttgggt gttcatcgtc agcggtgcca gcttcatcat gaactacttc accgattcgg
    46561 tggggatgtt gctgatgtat ctgccgcgca tgttgttcta taccgatccc atcgctaaag
    46621 gcggcttccc gcagggctgg accgtgttct actgggcatg gtgggtgatt tacgccatcc
    46681 agatgagtat cttcctcgcc cgcatctccc gtggtcgtac tgtgcgtgaa ctgtgcttcg
    46741 gcatggtgat ggggctgaca gcatccacct ggatcctgtg gaccgtgctc ggtagtaaca
    46801 ctctgctgtt gatggataaa aacatcatca acattccaaa tctgatcgaa cagtacggtg
    46861 tggcgcgcgc catcatcgaa acctgggccg ctctgccact cagcaccgcc accatgtggg
    46921 gcttcttcat cctctgcttt attgccaccg ttacgctggt taacgcctgc tcttataccc
    46981 tggcgatgtc tacttgccgc gaagtacgcg atggtgaaga accaccgctg ctggtgcgta
    47041 tcggttggtc aattctggtt ggcattatcg gtattgttct gctggcgctc ggcggcctga
    47101 aaccgattca aaccgccatt atcgccggag gatgcccgct gttcttcgtc aacattatgg
    47161 tgacgctctc ctttattaaa gacgcgaaac agaactggaa agattaatta acccccaaaa
    47221 tatcaagagg ttgaaagatg gattttaatt taaatgatga gcaggaactg tttgtcgccg
    47281 gtatccgcga actgatggcc agcgaaaact gggaggccta ttttgccgag tgcgaccgtg
    47341 acagcgtcta cccggaacgt tttgtcaaag cactggcgga tatgggtatc gacagtctgc
    47401 tgatccctga agagcacggt ggtctggacg cggggtttgt tactctcgcc gccgtgtgga
    47461 tggagctggg acgtctgggg gcaccaacct atgtactgta ccagctgccg ggcgggttca
    47521 ataccttcct gcgcgaaggc acacaagagc agatcgacaa gattatggct ttccgcggca
    47581 ccggtaagca gatgtggaac tcagcgatta ccgaaccggg cgcgggctcc gacgtgggta
    47641 gcctgaaaac gacttatacc cgtagaaatg gtaagattta tcttaatggt agtaagtgtt
    47701 ttattaccag tagcgcctac accccgtaca tcgtggtgat ggcgcgcgac ggggcttctc
    47761 cggacaaacc tgtctacacc gaatggtttg ttgatatgag caaaccgggc atcaaagtga
    47821 ccaaacttga gaagctcggt ctgcgtatgg atagctgctg tgaaatcacc tttgacgatg
    47881 tggaactgga cgagaaagac atgttcggtc gggaaggtaa cggctttaac cgcgtcaaag
    47941 aagagttcga ccatgaacgt ttcctggtag ccctgaccaa ctacggtacg gcgatgtgcg
    48001 cctttgaaga tgcggcgcgc tacgccaacc agcgcgtgca gtttggcgag gctattggtc
    48061 gtttccagtt gattcaggaa aaattcgccc acatggcgat caaattaaac tccatgaaaa
    48121 acatgctgta tgaagcagcg tggaaagcag acaacggcac catcacctct ggcgatgcag
    48181 cgatgtgcaa atacttctgc gccaatgcgg catttgaagt ggtggatagc gcaatgcagg
    48241 tgctgggcgg tgtcgggatt gcgggcaacc accgcatcag ccgcttctgg cgtgacctgc
    48301 gtgtagaccg cgtttccgga ggatctgacg aaatgcagat cctgacgctg ggtcgtgcgg
    48361 tgctgaagca ataccgctaa gtccgtgcgc tgcctgatgc gacgctgacg cgtcttatca
    48421 ggcctacggg agcacgaatg taagccggat aaggcgttta cgccgcatcc ggcagtcatg
    48481 cgctacataa atttttcagg agctaataat ggatcatcta cccatgccga aattcggacc
    48541 gctggccgga ttgcgcgttg tcttctccgg tatcgaaatc gccggaccgt ttgccggaca
    48601 aatgttcgca gaatggggcg cggaagttat ttggatcgag aacgtcgcct gggccgacac
    48661 cattcgcgtt caaccgaact acccgcagct ctcccgccgc aatttgcacg cgctgtcgtt
    48721 aaatattttc aaagatgaag gccgcgaagc gtttctgaaa ttaatggaaa ccaccgatat
    48781 cttcatcgaa gccagtaaag gtccggcctt tgcccgtcgt ggcattaccg atgaagtact
    48841 gtggcagcac aacccgaaac tggttatcgc tcacctgtcc ggttttggtc agtacggcac
    48901 cgaggagtac accaatcttc cggcctataa caccatcgcc caggccttca gtggttacct
    48961 gattcagaac ggtgatgttg accagccaat gcctgccttc ccgtataccg ccgattactt
    49021 ttctggcctg accgccacca cggcggcgct ggcagcactg cataaagtgc gtgaaaccgg
    49081 taaaggcgaa agtatcgaca tcgccatgta tgaagtgatg ctgcgtatgg gccagtactt
    49141 catgatggat tacttcaacg gcggcgaaat gtgcccgcgc atgagcaaag gtaaagatcc
    49201 ctactacgcc ggttgcggtc tgtataaatg tgccgacggc tacatcgtga tggaactggt
    49261 gggcattacc caaattgaag agtgctttaa agatattggc ctcgcacatc tactcggtac
    49321 gccggaaatc ccggaaggca ctcagcttat ccaccgtatc gaatgccctt acggcccact
    49381 ggtagaagaa aaactcgatg cctggctggc ggcacatacc atcgcagaag ttaaagaacg
    49441 ctttgccgaa ctgaatatcg cctgtgccaa agtactgacc gtaccggaac tggaaagcaa
    49501 tccacagtat gttgcccgtg aatcaatcac tcagtggcaa acgatggatg gtcgcacctg
    49561 caaagggccg aacatcatgc cgaaattcaa aaataacccc ggacaaatct ggcgcggaat
    49621 gccctcacat ggcatggaca cggctgccat tttgaaaaat atcggctaca gcgaaaacga
    49681 cattcaggag ttggtcagca aaggtctggc caaagttgag gactaaacgc gtggcgtcag
    49741 tcggcttgcc tgactagcgc ccgcccaata acaaacgaac aaaaatggat agaggtgcaa
    49801 tggatatcat tggcggacaa catctacgtc aaatgtggga cgatctggcg gacgtttacg
    49861 gtcataaaac ggcgctgatt tgtgaatcca gcggcggagt cgttaaccgg tatagttatc
    49921 ttgagttaaa tcaggagatt aaccgcacgg caaacctgtt ttatacgctg gggattcgca
    49981 aaggcaacaa ggttgcacta catctcgaca actgcccgga atttatcttt tgctggttcg
    50041 ggctggcaaa aattggcgcg attatggtgc cgattaacgc ccgcctgtta cgcgaggaaa
    50101 gcgagtggat cttgcaaaat agccaggcgt gcctgctggt gaccagtgcg caattttatc
    50161 ctatgtatca acagattcag caggaagatg ccactcaatt gcggcacatt tgcctgacag
    50221 atgtggcact tcccgctgat gatggagtga gttcatttac tcaactgaaa aatcaacaac
    50281 ctgccacctt gtgctatgca ccgccgctat cgactgacga tacggcggaa attctcttca
    50341 cctccggcac cacctcccga ccgaaaggtg tggtgattac ccattacaac ctgcgcttcg
    50401 ctggatatta ctccgcctgg cagtgtgcac tgcgtgacga tgacgtctac atgacggtaa
    50461 tgcctgcgtt tcatatcgat tgccagtgta ctgcggcgat ggcggcgttt tctgccgggg
    50521 ccacctttgt gctggtcgag aaatacagcg cccgcgcctt ctggggacag gcgcagaagt
    50581 accgcgccac cattaccgaa tgtattccga tgatgatccg tacgttgatg gtgcagccgc
    50641 cttcagcgaa cgatcggcaa caccgcctgc gggaagtgat gttttatctc aacttgtcgg
    50701 agcaggaaaa agacgcattt tgtgaacgct tcggcgttcg cttgctgacg tcttatggga
    50761 tgacggaaac cattgtgggc attatcggcg atcgccctag cgataaacga cgctggccgt
    50821 cgattggtcg ggcggggttt tgctacgaag cggagatccg cgacgatcac aatcgcccgc
    50881 tcccggcagg tgagatcggt gaaatctgta ttaaaggcgt accagggaaa accatcttca
    50941 aagagtattt tctcaacccg aaagccactg cgaaagtgct ggaagccgac ggctggctac
    51001 ataccggcga taccggatac cgcgacgaag aaggcttttt ttatttcgtc gatcgccgct
    51061 gcaatatgat caaacgcggt ggcgagaatg tctcctgcgt ggagctggaa aatatcatcg
    51121 ccacccatcc gaaaattcag gacatcgtgg ttgtgggtat taaagattcg attcgcgatg
    51181 aagccatcaa agcatttgtg gtgctgaatg aaggtgaaac attgagcgaa gaggaatttt
    51241 ttcgcttctg cgaacaaaat atggcgaaat ttaaagtgcc ctcttatctg gagatcagaa
    51301 aagatctgcc acgtaattgc tcggggaaaa taattagaaa gaatctgaaa taaatgaaac
    51361 gtcagggaac taccctgcca gctaataatc acgccctaaa acaatatgca ttttttgcag
    51421 ggatgttgtc gtccctaaaa aagcaaaaat ggagaaaagg aatgagtgaa tcattacatc
    51481 tgacccgcaa tggatcaatt ctggaaatta cccttgatcg cccaaaagcg aatgctattg
    51541 atgcaaaaac cagctttgaa atgggcgaag tatttctaaa tttccgtgac gatccgcaat
    51601 tacgtgtcgc cattattacc ggtgccggag agaaattctt ttccgcgggc tgggatttaa
    51661 aagcggcagc agaaggcgaa gcaccggatg ctgactttgg cccgggtggt tttgcgggat
    51721 taaccgaaat tttcaatctc gacaaaccag ttatcgcagc tgtgaacggc tacgcctttg
    51781 gcggcggttt cgaactggcg ctggcggcag attttattgt ttgtgccgat aacgccagct
    51841 tcgccctgcc ggaagccaaa ctgggcatcg ttcctgacag cggcggtgtg ctgcgtctgc
    51901 cgaagatcct gccgcctacc atcgtcaatg aaatggtgat gaccggcaga cgaatgggcg
    51961 cagaagaggc gctgcgttgg gggatagtca accgcgtggt tagccaggcg gaactgatgg
    52021 ataacgcccg cgaactggct cagcagctgg ttaacagcgc cccgctggcg attgcggcgc
    52081 tgaaagagat ctaccgcacc accagcgaaa tgccggtaga agaagcgtat cgctatattc
    52141 gcagcggcgt gttgaaacac tatccatcgg ttctgcattc ggaagatgcc attgaagggc
    52201 cgctggcgtt tgccgagaag cgcgatccgg tgtggaaagg acgttaacaa ccgtgagcta
    52261 ttacgccttt gaggggttaa ttccggtggt tcacccgacg gcgtttgtcc atcccagtgc
    52321 cgtcttgatt ggcgatgtga ttgtgggagc cggtgtctac atcggcccac tcgcctcact
    52381 gcgtggtgac tacgggcggt tgatcgtgca agcgggagcc aatattcagg atggctgcat
    52441 tatgcatggc tactgcgaca ctgacaccat cgttggggaa aacggccata tcgggcatgg
    52501 agcgatcctg catggttgtg tgattggtcg cgatgcattg gtcgggatga acagcgtaat
    52561 tatggatggc gcggtcattg gcgaagagag cattgttgcc gcaatgagct ttgtcaaagc
    52621 aggctttagt ggcgagaaac gccagttgtt gatgggtacg ccagcccgcg ctgtacgcag
    52681 tgttagtgac gacgagttac actggaaacg gttgaatacc aaagagtatc aggatcttgt
    52741 cgggcgctgt catgcatcgt tacatgaaac gcagccactg aggcaaatgg aggaaaatcg
    52801 cccccgtttg caggggacga cggatgtgac gcctaaacgg tgatcttgcc ggatgcgacc
    52861 tggttgctgg ataagatgct acaacatcgt atctggcaag caggacgccc ggtatcaacg
    52921 agataaaatt aacgacgcat actctttgac aacatcatct gccacttctg ctcgcggtta
    52981 agttccgtga gcggaacggc aggaatacgc ggtgtctttc ttacgccgac taatttttcc
    53041 cgactgttat tgcgcaggcg cgcgtaaatt tgctcatcga tatcgaccac tttaaccagt
    53101 cgctgacact ggcatccccg cccttccagc ttattaggga tcatcttaac ttcgcagctt
    53161 atttctgtga cttccgacag aatataagtc agggtattaa ccgccttgct gtgttcaata
    53221 tcgaaatgaa tagagatctc tcttgctatc acccaccgat tttcagccat catccattcg
    53281 gcgattaaca agtagagtgg tttttcaaca tatccttcac acatacgaac tccagtttca
    53341 caaattaaaa gtctggctat gactcgtttt gaagcaatta aacaaggcca tattaaaatt
    53401 gtggatatat cgatagtatg caacttcacc gtcgataagt gtgagctaaa tcctgcttat
    53461 gtcatcaaaa atatcgattc cccaaaagat ttacttaatg ggcaaaaaaa aacggtcctc
    53521 atcagagaac cgtattagtt attcgatcaa attagcggat gaaaaatatc cgccacgaca
    53581 cgctattatt tgatctgtgc gtgcatttcc tgcaccgaaa ttactttttc agtcgcatcg
    53641 gcattcagcg ccatcgcggt agcgaaacca ccgttcaggg tggtgtcata atgcacttta
    53701 tattgcagcg cactgcgacg gatcacgcgg gagtcttcaa tcgcacgacg gcctgaggtg
    53761 gtgttgatga tgtaggtata ttcgccattc ttgatacggt cctgaatgtg cggacggcct
    53821 tcatgcacct tgtttaccag acgcggattg atacccgctt cgcccagcac aatcgccgtg
    53881 ccgtgggtcg catccagctc gaagccctgt ttcagcagtt ttgccgccag gtccaccacg
    53941 cgttctttat cgccttcgcg cacggaaagc agcgcacgac cgtgtttctt catggtggag
    54001 ttgctgccca gctgcgcttt ggcgaacgct tcagcgaagg tgcggcccac gcccatgact
    54061 tccccggtag agcgcatttc tggccctaac agcgggtcaa cgcctgggaa tttattgaac
    54121 ggcagcacca cttctttcac cgagtagtac ggcggaataa cttctttggt tacgccctgc
    54181 tcagccagcg atttgccagc catcacacgc gccgccactt ttgccagcgg tacgccggtg
    54241 gctttggaga cgaacggaac agtacgcgcc gcacgcgggt ttacttcaat caggtaaact
    54301 tcgttgtttt tcaccgcaaa ctgcacgttc atcagaccgc gcacctgcaa ttcgaaggcc
    54361 agtttctgca cctgctggcg catcacatcc tgaatttcct gacttaaggt gtacgctggc
    54421 agcgaacatg cggagtcacc ggagtgcaca cccgcctgct cgatgtgctc catgatgccg
    54481 ccaatcagca ccatttcgcc gtcgcagatg gcatccacgt caacttctac tgcgtcatca
    54541 aggaaatgat ccagcaacac tggcgcatcg ttagacacgc taaccgccgt ctggaagtag
    54601 cgacgcaggt cagcttcgtc atagacgatt tccatcgccc gaccgccgag aacgtaagac
    54661 ggacgcacca ccagcgggta gccaatctct tttgccttct caactgccat ttcaatagca
    54721 gtaacggtgg cgttcgccgg ttgtttcagt ttcagacggt caaccgcatg ctggaagcgt
    54781 tcacggtctt ccgcacggtc gatagcatcc gggctggtgc cgataaccgg tacgccagcg
    54841 gcttccagtg cacgcgccag tttcagcggg gtctgaccgc cgtactgaac gataacgcct
    54901 ttcggcttct caatacgcac gatttccagc acatcttcca gagttaccgg ctcgaagtag
    54961 aggcgatcgg aagtgtcgta gtcggtagag acggtttccg ggttacagtt aaccataatg
    55021 gtttcgtaac cgtcttcacg cagcgccagc gaggcgtgta cgcagcagta gtcgaattcg
    55081 ataccctgac cgatacggtt tggaccgccg ccaagcacca tgattttttc acggtcggta
    55141 gacggattcg cttcgcactc ttcttcataa gtggagtaca tgtaagcggt gtcggtggca
    55201 aactctgccg cacaggtatc cacgcgcttg tagaccgggt gcaggtcata ttggtcacgc
    55261 agcttacgga tttccgcttc gcgtacgccc gccagttttg ccaagcgcgc atcggcaaag
    55321 cctttgcgtt tcagctggcg caggaattca gcgttcaggc cagtgatgcc cacttccgcc
    55381 actttctctt ccagacgcac cagttcttca atctgtacca ggaaccagcg gtcaatgtta
    55441 gtcaggttga agacgccgtc cacagacagg cccgcgcgga aagcatcggc gatgtaccag
    55501 atacgctctg cgcctgcgtc tttcagttcg cgacggattt tggttaacgc ttccgggtca
    55561 tccaggctca ctttcgggtc gaagccagtt gcgcccactt ccaggccgcg cagcgctttt
    55621 tgcagggatt cctgctgcgt gcgaccaatc gccatcactt cgccaaccga tttcatctga
    55681 gtggtcagac ggtcgttagc accggcgaat ttttcgaagt tgaagcgagg aattttagta
    55741 accacatagt cgatggacgg ctcgaaggag gccggagtac gtccgccagt gatgtcgttc
    55801 atcagttcgt ctagggtgta acccaccgcc agtttcgccg ccactttagc aatcgggaaa
    55861 ccggtcgctt tcgacgccag cgccgaagaa cgggacacgc gtgggttcat ttcgataaca
    55921 atcaggcgac cgtttttcgg gttcaccgca aactggacat tggaaccacc ggtttcaacg
    55981 ccgatttcac gcagcaccgc catcgaggcg ttacgcatga tttgatattc tttgtcggtc
    56041 agcgtttggg ctggcgcgac agtgatggag tcaccggtgt ggatgcccat cgcatcaaag
    56101 ttttcgatag agcagacgat gatgcagttg tcgtttttat cacgcaccac ttccatctcg
    56161 tactctttcc agccgatcag cgactcatca atcagcaact ctttggtcgg agagagatcc
    56221 agaccgcggg cgcaaatttc ttcaaactct tcacggttat aagcgatacc gccgccgcta
    56281 ccgcccatgg taaaggatgg gcgaataatg cacgggaagc ccacgtcagc ggcaacagcc
    56341 agcgcttctt ccatcgtgtg tgcgataccg gaacgcgcgg tttccagacc aattttcttc
    56401 atcgctacgt cgaaacgacg gcggtcttct gctttatcaa tcgcatcggc agtggcacca
    56461 atcatggtga caccgaactc ttccaacacg ccctgacgtt ccagctccag cgcgcagttc
    56521 agcgccgtct gaccgcccat ggttggcagc accgcgtccg ggcgctcttt ttcaataatc
    56581 ttgcgtacca cttcccagtg aatcggctcg atgtaggtcg catcggccat ttccgggtcg
    56641 gtcatgatgg tcgccgggtt ggagttcacc agaatgacgc ggtaaccctc ttcgcgcagg
    56701 gctttacatg cttgcgcgcc agagtagtca aactcacacg cctgaccgat aacaatcggg
    56761 cccgcaccca gaatcaggat actttttata tctgtacgtt ttggcatggc tcttttactc
    56821 ctgattactt agcggtttta cggtactgct caattaactt gataaagtgg tcgaacaacg
    56881 gcgcggcgtc gtgtggacca gggctggctt cagggtgacc ctggaagctg aacgccggct
    56941 tatcgatgcg atgaatgccc tgtaacgtac cgtcgaacag ggatttatgc gtgacacgca
    57001 ggtttgcagg taatgttgct tcatccaccg caaaaccgtg gttctgggcg gtgatcatca
    57061 ccacgttttt ctccacatct ttaaccggat ggttgccgcc gtggtgacca aatttcattt
    57121 tgacagtctt cgcaccgctc gccagcgcca gcagctgatg accgagacag atgccgaata
    57181 ccggaatatc ggtttcgagg aacttctgga tggcggtaat ggcgtaatcg cacggggccg
    57241 ggtcgccagg accgttggag aggaagatgc cgtctggatt cattttcagc acatcttccg
    57301 cagaagtttg cgccggaacg atggtcaggc gacagcctct atccaccagc atccgcagga
    57361 tgttgcgctt ggcaccaaaa tcataagcca cgacgtggaa cggcagctcg tcttcttttt
    57421 tcgcttctgg caggccgccg gtcaacgtcc agctcccttg tgtccagcta taggcttctg
    57481 cggtggtcac ttcttttgcc agatccatgc cgttcagacc tgggaacgcg cgggcttttt
    57541 ctaacgccag cgccgcatcc gggttatcgc ctgcgataat gcagccattc tgtgcgcctt
    57601 tctcgcgcag taaacgcgtc agcttacggg tatcgatatc ggcaatcgcc acgatgttat
    57661 ggcgcttcag gtaagaagag aggtcttcgg tattacggaa gttgctggca atcagcggca
    57721 ggtcgcgaat caccagacct tgtgcatgta cctgagaaga ttcttcatcg gcgtcattag
    57781 tgccgacatt gccaatatgg ggataagtaa gagtaacgat ttgacgagaa taggaaggat
    57841 cagtgaggat ttcttgataa ccggtcattg aagtattgaa aacgacttcc ccaaccgccg
    57901 aacctgttgc ccctatggcc cgaccgtgaa actgggttcc gtcttccaga accaatagcg
    57961 ctgacttaat caaaacaccc tccagagaat actcactcac tttatttgca tattaattca
    58021 cagtgatgac gtaaatcaat gcaaatctgc ttaccgacga atttctggca aacggcggca
    58081 ttctggagat atgggcgcta aaagtcaact taatggcagg attttttata ttattttgtg
    58141 gctcatgcat gttttacagg cattaagcga caaaaacatc atctaacctg ttctggaaaa
    58201 cgcttgcgca gcaagaagat tgcatgaaat aaaaacgagc aggaaaaagt ggaccaaatg
    58261 gtcaaaattt acattaaaac aacaaaaata gaagacgaaa catgtaattt ttggcagtta
    58321 tagaaaattt aaaagcataa caccaaaaat aaaagggcta aaaattaaag ccctttataa
    58381 ttaataaatt aaatgtgtta cttttgcatc ataataaaca ttttgtggtt acaaattatt
    58441 gagatcaagc acatctcgca tatcaaaaag accactttcc ttaccactca accacaaagc
    58501 cgatcttacc gcaccgttag caaatgtcat gcggctggac gccttatggg tgatctccag
    58561 acgctcgcca atatcggcaa acatcgcggt atgttcgcca acgatgtcac ctgcacgcac
    58621 ggtagcaaaa ccaatggtac ctggcacacg ttcaccggta tggccttcac gactgtagac
    58681 cgcgcaatct ttcagatctt tatcaagggc gtgggcgatc gcctctccca ttgccagtgc
    58741 ggtgcctgac ggcgcatcaa ctttatgtct atgatgtgct tcaataattt cgatatcggt
    58801 gtagtcaccc atcactttgg ctgctttctc cagcagctta agcatgacgt taacgccaac
    58861 gctaaaattg gcggcaaaga caatcgcaat atcggcggcg gcgtcacgaa ttgcctgttt
    58921 accggcttcg tcaaaccccg tagtgccgat caccatccct ttgccatgct gccgacaaaa
    58981 agcgagatgg ttcagcgtac cttccggacg ggtaaaatcg ataaacacat caaaatcatc
    59041 ttttatcgca tcaagactgc tttgcacggt aacgcctgtt ttccctgcac cagccagctc
    59101 accggcgtcg ctgcccagta aagaagatcc ttcgcgctcc agcgccgcgc ccagctgcac
    59161 gccttctaat gccagtgccg cctgaatcaa ctggcggccc atacgccccc cggctcccgc
    59221 gatggcaacg cggatgtttg catcatgcat agctattctc ttttgttaat ttgcatagac
    59281 cgttttcaga gtaaccagcg catcgctacg ccgccagcca aaaacgctga taattagaaa
    59341 ttaatgataa acagggaggg atatcagtga aaggcatgag tcgatgaatg actgcatgcc
    59401 gataacatgt gacaggttac ggcaccagag ccagcacctc agccacccac tgttggaagc
    59461 cttttacatt cagatccaat gccacctgta cattggctgg tttgcccagg caaccgtcga
    59521 tatcaaccac cgtcgtgcct gaggtaaatt cgccttgagt ttctaccgcg acaaaacacg
    59581 gcttgagggt gaataagtcc gggcgcacca gccaggcgat ggcgcagaga tcgtgcattc
    59641 gcagaccgct ttgcatactg ccgctacggt agtggctaaa cagggcgtgg agcatttttc
    59701 cggtgcgatt caactccggt aaagtagcga gatagtcagg ggttaatatt gcctgattgg
    59761 tgacatccag accgcacatg acaatttcga tgccgctacg gaagacacag gcagccgctt
    59821 ctggatcggc agcgatatta aactcggcat ttggtgtaca gttgccgcga ccagcagaac
    59881 cgcccatgat caccagacga cgaatatacg gtttacattc cgggcattgt aaaagtaaca
    59941 gcgcaatatt ggttaacggg ccgatggcca ccagggtaac gggctctggt gcacgcatca
    60001 gggcatcccg aatcgccaga aacgccggta tcccgagtgg ctttcggttg tgctcaacaa
    60061 agtcgtagcc agccattccc gattcgccgt gcacagatgc tgcatcacgc ggtgcgcgta
    60121 ccagcggcac agcggctccc tgggcgagcg gaatctccgc attccagaaa tgcagcagtt
    60181 gcagggcatt gcgggtagtt ttctcaaccg agacattacc cgcgacggtg gtcatcagtt
    60241 gcaggtcgag ttcgggggca aaaatcgcgg cggcaatggc gacggcatcg tcaatgccgg
    60301 ggtcagtatc gaggaagata ggtaaacgca tgttttcttc ataaaaaatg ccggtaacaa
    60361 gaccggcatt ttcgcataac ttaggctgct aatgacttaa tcgacttcac gaatatcgac
    60421 acgcagctct ttcggcactt cgaaaacaat attttcttca cggccttcca gcggaatggc
    60481 ttcaccacca cccaactgct gcaaacgtgc caccacattc tgcaccagaa tatccggagc
    60541 cgatgcgccc gcagtcacgc cgacgcattt aacctctttc acccactctt cctggatatc
    60601 tttcgcatcg tcaatcaaaa acgcgtgttt gcccatacgc tgggccagct ccgccagacg
    60661 gttggagttg gaggagtttt tcgaaccgac caccaacaca acttccgcct gttctgccag
    60721 ggcgcgtacc gcttcctgac ggttagtcgt ggcgtagcag atgtcatctt tgcgcggacc
    60781 gacaattttc gggaagcgtt tacgcagcgc gtcgatcaca tcagacgtgt catccaccga
    60841 cagcgtggtt tgggtcataa aggagagctt ctcttcgttt ttgaccgtca gtttccacac
    60901 atcgtctggc gattcgacca gatacattcc cccttcgggg ttgctgtact gccccatcgt
    60961 cccttccact tccgggtgcc cggcgtggcc gatgagaata gattcttcgc cacggcgact
    61021 ggcgcgggca acttccatat gcactttggt caccagcgga caggtggcat caaacaccgt
    61081 caaatcgcgg cttttcgctt cgttacgaac cgcctgagaa acaccgtgtg cggagaaaat
    61141 caggatcgcg ccgtccggca cttcgctaat ctgctcaata aagatagccc cacgctcgcg
    61201 caagctatcg accacgtagc ggttatgcac cacttcgtga cggacatata tcggtgcgcc
    61261 gtaaatggcc agcgcgtttt caacaatgct gatagcgcgg tctaccccgg cacaaaaacc
    61321 acgcgggttg gccaacagga tctgcatgtt acgcctccag tgccggatcg atttccagca
    61381 cttcaatatc aaaatgaacg gtctgcccgg ccagcggatg gttgaaatca acggtaatgg
    61441 agtcgccgtt aatttcgcgg atcacaccag gcatctcact gccatccatt gcggtaaaaa
    61501 gcatgattgc gccaatttct ggctcgcctg catccataaa ttcacggcgg gagaagtact
    61561 gaatcaggtc cggtgacggc acgccaaacg ccgcatctgg ctccagcgag aaggtggttt
    61621 tgtcgcccac tttcagcccc agcaggtgtt gctccagccc ttcagaaaga gaagcatcgc
    61681 ccaggcggaa cagcgccggt ttaccgttgt tgcgagtaga ctcggcggtg gtgccatcgt
    61741 cgagttttag cgtgaagtgc accaggacgg cgctattgct ctgtacagat tcagacatgc
    61801 aggtttgctc ttatttttga ggatttttgc cggatgcggc ctacgatttc gtagcaatct
    61861 gtaggccgca tccggcaggg tttattattg ttttttcgct ttggaaggta aaaacccttc
    61921 cagcacgatc agtgccgcac cgacacaaat ggcagtatcg gcaaggttga aggtggcgaa
    61981 gtgccagtcg ccgacgtaga agtcgatcat atcgacaacg aagccgtgcc acaggcggtc
    62041 gaacaggttg cccagcgcgc cgccaataat cagcgcgtaa gcgatattgt ttagcttctg
    62101 cgtggccttc gagcgataca tcatcactgc caggagcacg ctaataccaa tcgcaatacc
    62161 ggcaaagaac caacgctgcc agccgccgct atcggcaagg aaactaaacg ccgcgccata
    62221 gttacgcgca taatgcagat taagcgacgg gaacagcggg accgtatccc ccagagcaaa
    62281 gttctggagg atcaggtatt tgctgcccag atcgataatc agcacgacta ccaccagcca
    62341 cagccagcgt agccctgttg aacagatcga ttgactcatc aggcaaactt acgtttttca
    62401 ccgtcaccgg cgacgttgct gacacagcgg ccgcagattt ctgcgtgttc cgccaccttg
    62461 ccgacatcct gggtgtagtg ccagcagcgt gggcacttct caccttcggc tttactcaac
    62521 gcgactttca gccctttgag tacttcgctc tgctgagcat cagcaggtgc gtcgttatag
    62581 tctgcaacgg tagcgccgga ggtcaacagg acaaatcgta attcatcgcc cagcgcggtc
    62641 agtttcgccg ccagttccgg ttctgcatac aaggttactg ccgcttccag cgagccaccc
    62701 actttcttgt cggcacgcgc ttgctcaatg actttgttca cttcgccacg cactttcaac
    62761 agctcgtccc agaacgcatc gttcattgct tcactgtctg ccagaccaaa caggccttcg
    62821 taccactcac cggtgaagac gtatttttca cgttcgcccg gcagatagcc ccacacttca
    62881 tcagcggtga aggagaggat tggtgccatc cagcgcacca gcgcttcagc gatgtgatac
    62941 agcgcagtct ggcagctacg acgcgccacg ctatccgctt tggcggtgta ctgacggtct
    63001 ttgatgatgt cgaggtagaa ggaacccatc tcaacggagc agaagcgcat cagacgctgt
    63061 accacttcgt ggaaatcgta tgcttcgtac gccttgagga tgtcttcctg tgccgctttc
    63121 gcacaaccta cggcccagcg atccagtacc accatctctt ccggtttcac catatctttt
    63181 gctggatcaa aaccattcag gtttgccagc aggaagcgcg cggtgttacg gatacgacga
    63241 tagctatcgg cagcacgttt caggatctcg tcagaaacgg ccatttcacc ggtgtagtcg
    63301 gttgatgcca cccacagacg cagaatatcc gcgcccagtt tgttcatcac atcctgcggc
    63361 gaaacggtgt tgccgatgga tttagacatc ttgcgaccct gaccatccac ggtaaagccg
    63421 tgggtcagta cctgacgata cggcgctttg cctttcatcg cggtggagat cattagggaa
    63481 gacatgaacc aaccacggtg ctggtcagaa ccttccagat acatgtccgc tgcgtgaccg
    63541 gcaaattccg gacgcacgtc aacaacagaa gagtgggtag atccggagtc aaaccatacg
    63601 tcgagagtat ccggcacttt cacatactgg tcagcttcgt cgccgaggat ctctttcgca
    63661 tcgagatccc accacgcctg gatgccgtca acttcaacgc gtttagccac ttcttccatc
    63721 agttcgaggg tacgcggatg cagctcttcc gtgtctttgt gcacgaacag tgacatcggt
    63781 acgccccagg tgcgctgacg ggagatacac cagtcaggac ggttagcaac catcgactcg
    63841 atacgcgcct ggccccagtc cgggagccac tgcacgcctt tgatctcttt cagtgactgc
    63901 gcacgcagac ctttctgatc catgctgacg aaccactgcg gtgtcgcgcg gaagatgatc
    63961 ggcgttttgt gacgccagca gcacggatag ctgtgctgca ttttctcaac gtgcagcagc
    64021 gcgccttttt cctgcagcag cgcaacgacg atgtcgttcg ctttgaagac gttcacgcca
    64081 tccagcgtcg gataagtgcc cggcagataa gtgccgtccg ggccaaccgg gttagcggtt
    64141 tccaggccgt atttctgacc gatcacatag tcgtccgggc cgtggccagg cgcggtgtga
    64201 acggcaccgg taccggcatc cagggtaacg tgatcgccga ggattgccgg aacgtcgaag
    64261 cccataaacg gatgggtaaa gcgcagcagc tcaagctctg caccttttac cgtgccgaga
    64321 atggtgtaat cggtcacgcc gatacgctgc attacgcttt caaccagatc tttcgccaga
    64381 atcacggcct gaccgtcgat ctgcaccagc gcatagtcga aatcaggtgc aatagagatt
    64441 gcgcggttcg caggcagagt ccacggcgta gtggtccaga tcaccagcga gattgggccg
    64501 ttaacgttgc ttacgccaaa tttcgctttc agcgcatcct gatcgaccgc ctggaaagcg
    64561 acgtcaatgg acggagaagt tttgtcgtaa tactcaactt ccgcttctgc cagtgcagaa
    64621 cggcagtcta cgcaccagtg caccggcttc gcgcctttgt gcaggtgacc attgccgatg
    64681 attttgccca gcgcgcggat gatgttggct tcagttttga agtccatagt cagatacggg
    64741 tgcgaccagt cgcccagcac gcccagacgg ataaagtctt tgcgttgacc gtcaacctgg
    64801 gtcgccgcgt attcgcggca cttggcgcgg aactcggcgg cggtgaattt ctcacccggc
    64861 ttaccgtatt cttgctctac tttcagctcg atcggcagac cgtggcagtc ccagccaggc
    64921 acatacggcg agtcataacc ggaaagccct ttggacttca cgataatgtc tttcagaatc
    64981 ttgttaaccg agtgaccaat atgaatgctg ccattcgcat aaggagggcc atcatgcaga
    65041 atgaaggttt ttttgccttt tttagccgca cggatgatgc cgtacagatc atcatcagtc
    65101 caacgcgcca gcattcccgg ttcgcgcttg gcgagatcgc cacgcatcgg gaaccctgtt
    65161 tccggcaaat tcagggttga tttatagtca ctcatcagat tctcggttcc gtgtttcggt
    65221 ttgattacat aacaggctta agccggtttt gttagcccaa aaaattcgcg ggcggttaat
    65281 tcatcacgcg caatctgcgc tttcagttcg tccagcgacg caaatcgctg ctcattgcgt
    65341 atttttttac gcagcactac ttgtatatgg cgaccgtaaa ggtccattgc aacatctaac
    65401 aaatgcactt ccagctgctg gcgaataccg gcaaccgttg ggcgtgttcc gatgtttgcc
    65461 acgccgggta acggcttttc accgaggccc agcacttcta ccgcataaac ccctttcacc
    65521 ggggaaacct gacggcgtag cggtacattc gccgtcggga aacctatagt gcgccctaat
    65581 tcatcaccgt ggactacacg cccggagatg gcaaacgggt gccccagtaa actttctgcc
    65641 agagccagat tgtcatcagc cagcgcctga cgcacggcgg tgctgctgat acgcacacca
    65701 ccttcgcaaa aagtttgcgt actggtgata tcgaagccgt attccatgcc agctttctgt
    65761 aataacaaga aatcgccttc acgaccagcg ccaaagcgga aatcatcacc tacggcaaga
    65821 aattttacgc gcaaatgctt caccaaaaga tcgctgatga aattttgcgc ggttaacgcc
    65881 gcaaaacgcc tgtcgaaacg cacgcacagc acgtaatcaa cgccacactc tgcaaggtaa
    65941 cgcagttttt cccgcagccg ggtcagtctt gccggggctt tatcggtagc aaacagttcc
    66001 agtggttgag gttcaaaaag catcaccatc accggtaagt tgcgcttgcg cccttcttcc
    66061 tgcaagccct gtaacagcgc gcgatgaccg cgatgcacgc cgtcgaaatt accaatagtc
    66121 agcacacacc cttcttgcgg ggcctggctg agattatgta tgccgcgtat cagcttcatg
    66181 tctggctcaa aacagtgaaa atcgtccgag tataccttgt acagcggtca aggttaaccg
    66241 gcgattgagt accgaaatca gaaagaagtg gcttttcatt gccatggagc aaatcacggg
    66301 aagaaactga ccgcctgctg caatttttat cgcggaaaag ctgtattcac accccgcaag
    66361 ctggtagaat cctgcgccat cactacgtaa cgagtgccgg cacattaacg gcgcttattt
    66421 gcacaaatcc attgacaaaa gaaggctaaa agggcatatt cctcggcctt tgaattgtcc
    66481 atatagaaca catttgggag ttggaccttg gctaatatca aatcagctaa gaagcgcgcc
    66541 attcagtctg aaaaggctcg taagcacaac gcaagccgtc gctctatgat gcgtactttc
    66601 atcaagaaag tatacgcagc tatcgaagct ggcgacaaag ctgctgcaca gaaagcattt
    66661 aacgaaatgc aaccgatcgt ggaccgtcag gctgctaaag gtctgatcca caaaaacaaa
    66721 gctgcacgtc ataaggctaa cctgactgca cagatcaaca aactggctta atcgccaatt
    66781 tgctgaagct ttgtgaaaaa gcccgcgcaa gcgggttttt ttatgcctgc tgcttttgtg
    66841 atgcgtgaga gaacacactg ccggttgcca gatagtcaaa agagacatgc cgttgtacta
    66901 tctgaactct tcgtggctta agaatcgtag aaccatcggc tgatacattc tgaacaagaa
    66961 tatctactca atctccagac tcccccacaa agaatatgga tattgtgata cacattgagg
    67021 tattaaactt aagaccactc tatttttgaa ttaaattcct atatacttta accattcata
    67081 aatatctcct cattagacct
//
LOCUS       DAJTQY010000009        65753 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.009, whole genome shotgun sequence.
ACCESSION   DAJTQY010000009 DAJTQY010000000
VERSION     DAJTQY010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 65753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65753
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.009"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(128..607)
                     /gene="folK"
                     /locus_tag="OO349_000594"
     CDS             complement(128..607)
                     /gene="folK"
                     /locus_tag="OO349_000594"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HCR5865786.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESRILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSPAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDKLSKW"
     gene            complement(604..2022)
                     /gene="pcnB"
                     /locus_tag="OO349_000595"
     CDS             complement(604..2022)
                     /gene="pcnB"
                     /locus_tag="OO349_000595"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HCR5865787.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTT"
     gene            complement(2061..2987)
                     /gene="gluQRS"
                     /locus_tag="OO349_000596"
     CDS             complement(2061..2987)
                     /gene="gluQRS"
                     /locus_tag="OO349_000596"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HCR5865788.1"
                     /translation="MLPPYFLFKEMTDTQYIGRFAPSPSGELHFGSLIAALGSYLQAR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWL
                     HEQGLSYYCTCTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDLLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQIPLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFS
                     VEQILQSAVKNWMLTAVPESAIVNSTFSNASC"
     gene            complement(3024..3479)
                     /gene="dksA"
                     /locus_tag="OO349_000597"
     CDS             complement(3024..3479)
                     /gene="dksA"
                     /locus_tag="OO349_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HCR5865789.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3657..4361)
                     /gene="sfsA"
                     /locus_tag="OO349_000598"
     CDS             complement(3657..4361)
                     /gene="sfsA"
                     /locus_tag="OO349_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HCR5865790.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGSERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4376..4906)
                     /gene="thpR"
                     /locus_tag="OO349_000599"
     CDS             complement(4376..4906)
                     /gene="thpR"
                     /locus_tag="OO349_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HCR5865791.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTFPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4980..7409
                     /gene="hrpB"
                     /locus_tag="OO349_000600"
     CDS             4980..7409
                     /gene="hrpB"
                     /locus_tag="OO349_000600"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HCR5865792.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGLNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDEAVAVATAEMVRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVVLCPLYGVLSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGISLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVNAKSDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANSMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLKLLSPAQRPLQITRDLGAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7605..10139
                     /gene="mrcB"
                     /locus_tag="OO349_000601"
     CDS             7605..10139
                     /gene="mrcB"
                     /locus_tag="OO349_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HCR5865793.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGGQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10359..12602
                     /gene="fhuA"
                     /locus_tag="OO349_000602"
     CDS             10359..12602
                     /gene="fhuA"
                     /locus_tag="OO349_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HCR5865794.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLLNLYNPVNPDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            12653..13450
                     /gene="fhuC"
                     /locus_tag="OO349_000603"
     CDS             12653..13450
                     /gene="fhuC"
                     /locus_tag="OO349_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HCR5865795.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRSGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13450..14340
                     /gene="fhuD"
                     /locus_tag="OO349_000604"
     CDS             13450..14340
                     /gene="fhuD"
                     /locus_tag="OO349_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HCR5865796.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKHPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRTGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14337..16319
                     /gene="fhuB"
                     /locus_tag="OO349_000605"
     CDS             14337..16319
                     /gene="fhuB"
                     /locus_tag="OO349_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HCR5865797.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPSAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLIFAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVATERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16354..17634)
                     /gene="hemL"
                     /locus_tag="OO349_000606"
     CDS             complement(16354..17634)
                     /gene="hemL"
                     /locus_tag="OO349_000606"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HCR5865798.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYFAVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGIHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17859..19280
                     /gene="clcA"
                     /locus_tag="OO349_000607"
     CDS             17859..19280
                     /gene="clcA"
                     /locus_tag="OO349_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HCR5865799.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMGLGREGPTVQIGGNISR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19304..19369
                     /locus_tag="OO349_000608"
     CDS             19304..19369
                     /locus_tag="OO349_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5865800.1"
                     /translation="MAIIIGLEFARLPMSFGAKYE"
     gene            19362..19706
                     /gene="erpA"
                     /locus_tag="OO349_000609"
     CDS             19362..19706
                     /gene="erpA"
                     /locus_tag="OO349_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HCR5865801.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19753..20376)
                     /locus_tag="OO349_000610"
     CDS             complement(19753..20376)
                     /locus_tag="OO349_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HCR5865802.1"
                     /translation="MLVYWLDIVGTAVFAIYGVLLAGKLRMDPFGVLVLGVVTAVGDG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLVREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20414..21214)
                     /gene="btuF"
                     /locus_tag="OO349_000611"
     CDS             complement(20414..21214)
                     /gene="btuF"
                     /locus_tag="OO349_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HCR5865803.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYVQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21207..21905)
                     /gene="mtnN"
                     /locus_tag="OO349_000612"
     CDS             complement(21207..21905)
                     /gene="mtnN"
                     /locus_tag="OO349_000612"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HCR5865804.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21989..23506
                     /gene="dgt"
                     /locus_tag="OO349_000613"
     CDS             21989..23506
                     /gene="dgt"
                     /locus_tag="OO349_000613"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HCR5865805.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23636..25060
                     /gene="degP"
                     /locus_tag="OO349_000614"
     CDS             23636..25060
                     /gene="degP"
                     /locus_tag="OO349_000614"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HCR5865806.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKKLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            25215..26371
                     /gene="cdaR"
                     /locus_tag="OO349_000615"
                     /pseudo
     CDS             25215..26371
                     /gene="cdaR"
                     /locus_tag="OO349_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
     gene            complement(26460..26846)
                     /locus_tag="OO349_000616"
     CDS             complement(26460..26846)
                     /locus_tag="OO349_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HCR5865807.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(27160..27984)
                     /gene="dapD"
                     /locus_tag="OO349_000617"
     CDS             complement(27160..27984)
                     /gene="dapD"
                     /locus_tag="OO349_000617"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HCR5865808.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(28015..30687)
                     /gene="glnD"
                     /locus_tag="OO349_000618"
     CDS             complement(28015..30687)
                     /gene="glnD"
                     /locus_tag="OO349_000618"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HCR5865809.1"
                     /translation="MNTLPEQYANTALSTLPGQPQNPCAWPRDELTVCGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPLDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPQARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(30749..31543)
                     /gene="map"
                     /locus_tag="OO349_000619"
     CDS             complement(30749..31543)
                     /gene="map"
                     /locus_tag="OO349_000619"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HCR5865810.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            31911..32636
                     /gene="rpsB"
                     /locus_tag="OO349_000620"
     CDS             31911..32636
                     /gene="rpsB"
                     /locus_tag="OO349_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HCR5865811.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIVSRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            32894..33745
                     /gene="tsf"
                     /locus_tag="OO349_000621"
     CDS             32894..33745
                     /gene="tsf"
                     /locus_tag="OO349_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HCR5865812.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            33892..34617
                     /gene="pyrH"
                     /locus_tag="OO349_000622"
     CDS             33892..34617
                     /gene="pyrH"
                     /locus_tag="OO349_000622"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HCR5865813.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDSTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            34909..35466
                     /gene="frr"
                     /locus_tag="OO349_000623"
     CDS             34909..35466
                     /gene="frr"
                     /locus_tag="OO349_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HCR5865814.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            35558..36754
                     /gene="ispC"
                     /locus_tag="OO349_000624"
     CDS             35558..36754
                     /gene="ispC"
                     /locus_tag="OO349_000624"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HCR5865815.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEEVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYERYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANASEVARKE
                     VMRLAS"
     gene            36943..37701
                     /gene="ispU"
                     /locus_tag="OO349_000625"
                     /gene_synonym="uppS"
     CDS             36943..37701
                     /gene="ispU"
                     /locus_tag="OO349_000625"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HCR5865816.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            37714..38571
                     /gene="cdsA"
                     /locus_tag="OO349_000626"
     CDS             37714..38571
                     /gene="cdsA"
                     /locus_tag="OO349_000626"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCR5865817.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            38583..39935
                     /gene="rseP"
                     /locus_tag="OO349_000627"
     CDS             38583..39935
                     /gene="rseP"
                     /locus_tag="OO349_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HCR5865818.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPGSKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            39965..42397
                     /gene="bamA"
                     /locus_tag="OO349_000628"
     CDS             39965..42397
                     /gene="bamA"
                     /locus_tag="OO349_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HCR5865819.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDVGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            42519..43004
                     /gene="skp"
                     /locus_tag="OO349_000629"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             42519..43004
                     /gene="skp"
                     /locus_tag="OO349_000629"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HCR5865820.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            43008..44033
                     /gene="lpxD"
                     /locus_tag="OO349_000630"
     CDS             43008..44033
                     /gene="lpxD"
                     /locus_tag="OO349_000630"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HCR5865821.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            44138..44593
                     /gene="fabZ"
                     /locus_tag="OO349_000631"
     CDS             44138..44593
                     /gene="fabZ"
                     /locus_tag="OO349_000631"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HCR5865822.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            44597..45385
                     /gene="lpxA"
                     /locus_tag="OO349_000632"
     CDS             44597..45385
                     /gene="lpxA"
                     /locus_tag="OO349_000632"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HCR5865823.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            45385..46533
                     /gene="lpxB"
                     /locus_tag="OO349_000633"
     CDS             45385..46533
                     /gene="lpxB"
                     /locus_tag="OO349_000633"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HCR5865824.1"
                     /translation="MTDQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNGARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            46530..47126
                     /gene="rnhB"
                     /locus_tag="OO349_000634"
     CDS             46530..47126
                     /gene="rnhB"
                     /locus_tag="OO349_000634"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HCR5865825.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEYGATEHHRRSFGPVKRALGLAS"
     gene            47163..50645
                     /gene="dnaE"
                     /locus_tag="OO349_000635"
     CDS             47163..50645
                     /gene="dnaE"
                     /locus_tag="OO349_000635"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HCR5865826.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLALLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTMRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKSVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            50658..51617
                     /gene="accA"
                     /locus_tag="OO349_000636"
     CDS             50658..51617
                     /gene="accA"
                     /locus_tag="OO349_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HCR5865827.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            51716..53857
                     /gene="ldcC"
                     /locus_tag="OO349_000637"
     CDS             51716..53857
                     /gene="ldcC"
                     /locus_tag="OO349_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HCR5865828.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVFFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECRPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYHNMRIQDLAQGIHKLIRKHDLSGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            53914..54303
                     /locus_tag="OO349_000638"
     CDS             53914..54303
                     /locus_tag="OO349_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR5865829.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54368..55666
                     /gene="tilS"
                     /locus_tag="OO349_000639"
     CDS             54368..55666
                     /gene="tilS"
                     /locus_tag="OO349_000639"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HCR5865830.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSATLCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVLWQTWLQPLELPA
                     GLGTVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(55715..55975)
                     /gene="rof"
                     /locus_tag="OO349_000640"
     CDS             complement(55715..55975)
                     /gene="rof"
                     /locus_tag="OO349_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HCR5865831.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLILTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(55962..56162)
                     /locus_tag="OO349_000641"
     CDS             complement(55962..56162)
                     /locus_tag="OO349_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HCR5865832.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            56328..56873
                     /locus_tag="OO349_000642"
     CDS             56328..56873
                     /locus_tag="OO349_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HCR5865833.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            56870..57292
                     /gene="arfB"
                     /locus_tag="OO349_000643"
     CDS             56870..57292
                     /gene="arfB"
                     /locus_tag="OO349_000643"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCR5865834.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTKEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            57306..58016
                     /gene="nlpE"
                     /locus_tag="OO349_000644"
                     /gene_synonym="cutF"
     CDS             57306..58016
                     /gene="nlpE"
                     /locus_tag="OO349_000644"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HCR5865835.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIEISLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(58171..58995)
                     /locus_tag="OO349_000645"
     CDS             complement(58171..58995)
                     /locus_tag="OO349_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HCR5865836.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITAPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIFLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSDLAKAQLSSVCEGDKKSWFCSEFVTDAFAKS
                     GHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(59048..60766)
                     /gene="proS"
                     /locus_tag="OO349_000646"
     CDS             complement(59048..60766)
                     /gene="proS"
                     /locus_tag="OO349_000646"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HCR5865837.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(60877..61584)
                     /gene="tsaA"
                     /locus_tag="OO349_000647"
     CDS             complement(60877..61584)
                     /gene="tsaA"
                     /locus_tag="OO349_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HCR5865838.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIASYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKGSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(61581..61985)
                     /gene="rcsF"
                     /locus_tag="OO349_000648"
     CDS             complement(61581..61985)
                     /gene="rcsF"
                     /locus_tag="OO349_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HCR5865839.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKQMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(62103..62918)
                     /gene="metQ"
                     /locus_tag="OO349_000649"
     CDS             complement(62103..62918)
                     /gene="metQ"
                     /locus_tag="OO349_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HCR5865840.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(62958..63611)
                     /gene="metI"
                     /locus_tag="OO349_000650"
     CDS             complement(62958..63611)
                     /gene="metI"
                     /locus_tag="OO349_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HCR5865841.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVCKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(63604..64635)
                     /gene="metN"
                     /locus_tag="OO349_000651"
     CDS             complement(63604..64635)
                     /gene="metN"
                     /locus_tag="OO349_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HCR5865842.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQTEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            64823..65398
                     /gene="gmhB"
                     /locus_tag="OO349_000652"
     CDS             64823..65398
                     /gene="gmhB"
                     /locus_tag="OO349_000652"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HCR5865843.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
BASE COUNT        15553 a        16289 c        18113 g        15798 t
ORIGIN      
        1 tgggattgat atttatctgc gggaatttat attatgtttc tgcaaaaatg tttaaactaa
       61 aatttttatt aaggaaagag aggatgtatg attttcagaa aatatttata ttcgtaatat
      121 aaataaatta ccatttgctt aatttgtcaa atgctcttgt atgtaagact tcacgcaacg
      181 tctccccatc aggaaacgcc agctccggtg caatctcaaa cagcggccac agcataaatc
      241 cacgattctt catatcgtag tgcggaacgg tcaggcgttc ggtatttatc acttcattac
      301 caaacagcat gatatcgaga tccagcgtgc gtggtcccca gcgttcagct ttacgaacac
      361 gaccctgttg cagttctatg cgctgtgtat ggttgagtaa ctcttccggg gcgggcgaag
      421 tttcaagcgc cacggcggcg ttgaggtaat cgggttgatc ttgcggcccc agcggtgggg
      481 tgcggtaaaa cgaagaaacg gcaagaatgc ggctttcagg gatatcgcct aatgctttca
      541 gggcagcatt gacctgctcc agcggagagg ccagattgct gcctatggca atatacgcca
      601 ctgtcatgtg gtaccctcac gacgcggtgc gcgtttgcgt ggacgacgag tacgacgacg
      661 cggtgacggt tcttcatcca gctcgttaag catccctttt tgatctggtg gtgcggaaac
      721 ctggaactca ccccaccatt tcaccagacg ctgcagttca gcattacgtt caacttcagc
      781 tcgcaaggcc aacaggtcat aagccgcacg gaacttagga tgctccagca gtttccatgc
      841 gcgtttaccc tgacgacggg acatacgcaa ctgcaactgc cagatatcgc gggttaacgt
      901 cgtcagacgt ttcgggattg ccagtgaacg gcaggcttcg tccagcacgt cgttcatcgc
      961 cagcgcgaaa gcgtcgtgat aggtcaggcc gctttcctgg gcgatcttct gtgccgtctc
     1021 cagcagtggg taccagaaca tggcggcaaa caagaacgcc gggttcacgc gcatatcgtt
     1081 atggatacgc gtatcggtat tcttcagcac ctgttcaatg atccgctcca tcgggctgtc
     1141 gccattttcc gtgaagtagc gggtaatggt cgggaacagc ggctggaaca gatgatattc
     1201 acacaacagc ttataggttt cgtaaccgta gcccgcttgt agcagtttaa gcgattcttc
     1261 aaacaggcgt gccggtggga tatcgttcag cagggtagcg aggcgaggga tcggttctgc
     1321 ggtttccggg ctgatgcgca tacccaattt ggcggcaaaa cgtaccgcgc gcagcatacg
     1381 taccggatct tcacggtagc gcgtttccgg gttaccaatc agacggataa cgccgtcctt
     1441 cagatccttc ataccaccaa cgtaatcacg gacggtaaaa tccgctacgc tgtaatacag
     1501 gctgttgata gtgaaatcgc ggcgctgggc gtcttcttcg atggagccga aaatgttgtc
     1561 gcgcagcaac atgccgtttt gcccgcgttg ggaggtcgtg cggtcgctga cgttaccttc
     1621 gtggtgtcca cggaaggtcg caacttcgat aatctccggg ccaaacatta catgagccag
     1681 acggaaacgg cgacccacca ggcggcagtt acggaacagt ttgcgcacct gctcaggcgt
     1741 ggcgttagtg gttacgtcaa aatctttcgg ctttttgcca agtaacaggt cgcgcacgcc
     1801 gccgccaacc agccaggctt cgtatcccgc tttgttgagc ctgtacatta ccttcagggc
     1861 attttcactg atatctttgc gggaaatagc atgctgctca cgcgggatca ccgtcacctg
     1921 tggacgggcg actgcctgtt cagcctcgct ttcctcgcgg cttagcacct tgcggcaaaa
     1981 attagcgact cgggtaaaaa tagtacacct cggtagtgtc aaacatcatt caggacaaaa
     2041 aaatagcggc taatcatagc tcagcatgac gcatttgaga atgttgaatt tacaattgcc
     2101 gactcgggca cggcggtcag catccagttt ttgacggctg actgaaggat ttgctcgacg
     2161 ctgaaatcct gccagtgtgc ttctgcctgc tgccccagaa attgaagtgc cgcgattagt
     2221 accgggcgtg gatcgccttt cggcaacgca ggcgcatgat tctgcttgga aagtttagcg
     2281 ccttgtggat taagcgccag cggcagatga atgtaatcag gcactttcca gccaaaaagc
     2341 tggtacagcg ggatttgcct tactgttggt tcaatcagat cagccccacg cactatttct
     2401 gtaacgccct ggaaatgatc atcaaccaca acagccaggt tgtaggcgaa caacccatca
     2461 cggcgatgaa tgataaaatc ttcccgtgcc agtttttcgt cggcgtgaat aatgcctcgc
     2521 agcaggtcag taaattgcgt gaccggatgc tgctggcgga tacgcactgc ggcgttgtct
     2581 ggtccatgat gcaacacccg gcaatgaccg tcgtaaatac cgccaatgct ttgaatacgc
     2641 gcacgcgtac aggtgcagta ataacttagt ccttgttcat gtaaccaggc gagtgcttca
     2701 cgataggcgt cgtgacgttg cgattgccag agaacatcgc cgtcccagtg cagaccgtaa
     2761 tgttccagct ggcgcaggat agtttctgcg gcaccgggaa cttcacgagg cgggtcgata
     2821 tcttctatgc gtaccagcca gcgaccttgc cgggcgcgag cctgcaaata gctgccgagc
     2881 gcggcgatca gagagccaaa atgaagctcg ccggaaggag agggggcgaa gcggccaata
     2941 tactgtgtgt ctgtcatctc tttgaacaaa aaataaggcg ggagcatttc ccgcctgtgg
     3001 taaacgtgat ggaacggctg taattagcca gccatctgtt tttcgcgaat ttcagccagc
     3061 gttttgcagt cgatgcacag atcggctgtc gggcgcgctt ccagacggcg aataccaatt
     3121 tcaacaccgc aggattcgca gtagccgaaa tcttcgtctt ccactttttt cagcgtcttc
     3181 tcgatctttt tgatcagctt acgctcgcga tcgcggttac gcagttcgag gctgaactct
     3241 tcttcctggg ctgcacggtc taccgggtcc gggaagttgg ctgcttcatc ctgcatatgt
     3301 gtaacggtgc gatcgacttc atccctgagt tgattacgcc atgcttccag aatacgacgg
     3361 aagtgcgcca gctgggcttc attcatatac tcttcgcccg gcttctcctg atatggttcc
     3421 accccagcga tggcgagaat actcagggac gatgttttac ggttttgccc ttcttgcatg
     3481 ttgcttctcc ttaacacgca ctatcgatcc ccatgttcgg gggaaaaatg aggccgctat
     3541 aaatagcaga tgcttttccg gatagcaatt atctaaacgt aacacttgac aactgtgtga
     3601 ggaaaagcgt atttgcgcac gcgaccagaa tgtaaattaa ccagttactt actttactac
     3661 aatgtaaccg gcagtgattt tttaagagcc atgccttcag cagaaatttc cgctttgtaa
     3721 gccagaattt ctacccccct ctgttgagct tctgacaata gttgcgcgta tttctcatcg
     3781 atgtggcgcg cgggtgaaaa ccgtgtaatg gctgaatgca gcacggcgaa aaaaataacc
     3841 gcacgctggc cttcagccgc tacgctcatc aactcccgaa ggtgtttctg acctcgttca
     3901 gtgaccgcat cgggaaaata tccctgttcg ttctccgcta acgtaaccga tttcacttca
     3961 atatagcagt ctggacgcga atccgcctgc aacataaagt caatacggct gcgttcggag
     4021 ccgtatttta cttcgctttt cagcgagcta tagcctgaca gttctgaaat tgattcatta
     4081 aggatagcct ctttcgtcaa cctgttagcc caaagcgtat tgacgcaaat aaatgcgccg
     4141 ctctggcttt gagttaattc ccaggtgtgt gggtatttcc gtttggtgtt gtctgaagtc
     4201 gaataccaga cggtatcgcc aggcgttgca caaccggtca tcgcacccgt attcgggcag
     4261 tgtagcgtta attcgcgacc atcgggtgtg atcacatcgg ctaaaaaacg tttgtaacgc
     4321 tgaattagcg tcgcgcgctg tagaggggga gaaaattcca ttgcgacaat ccttgttatt
     4381 gcgttagcgc ccagcgtttt agcggcgtgt agcgtgtgcg tccacgggca aacgaggagg
     4441 cgtaaagggt gaactccgtc accgcataag accagttaaa acccggcggc gggaatgtca
     4501 ccgcctcgct ggcgtcgcgc aataaggtaa tatgtggatg aaacggacga ttgctttgaa
     4561 aacaaccgct gcgggcagcc tgtgaacgga gcatattcgc cagctggatt aagccgcgtg
     4621 gaggctgacg catccctaac cacaccacac gcgaacgcag ccattgtccg gcgtcatcaa
     4681 gcgtgagtgt gaaaccaggt tgacgaatcc gtccggctaa aagagaaagc gccttctctt
     4741 tctctgcgct cacttcgcct aaaaatgcca gagtcaggtg caaattatcg gcggcgaccg
     4801 gacgtcccgc ctcaggtggg aagtgtgtgg cgcgccaatg gataatctgt tcgcggattt
     4861 ctgcaggtaa gtcgatagca aagaacagac gttgcggttc agacatgtga ggcactcggt
     4921 tatgaattac cgcgatgcta caatgtggcg cgaagaatgt taaccctctg gagcgttttg
     4981 tgtcgtcgtt gcccgttgct gccgtcttac ctgaattact taccgctctc gattgtgcgc
     5041 cgcaggtatt attaagtgcg ccgaccgggg ccgggaaatc aacctggctg ccgctgcaac
     5101 tgctggcgca tcccggcatt aacgggaaaa ttatcctgct ggagccgcgt cgtctggcgg
     5161 cgcgtaacgt cgcgcaacgg ctggcggagc tgcttaacga aaagccaggc gataccgttg
     5221 gctaccggat gcgtgcgcaa aactgcgtcg ggctgaatac ccgcctggaa gtggttaccg
     5281 aaggcgtgct gacgcggatg atccagcgtg acccggaact gagcggtgtt ggattggtga
     5341 tccttgatga gtttcatgag cgcagtttgc aggcggattt ggcgttggcg ctgttactcg
     5401 atgtgcaaca aggtctgcgt gatgacctta aactgctgat tatgtcggct acgctggaca
     5461 atgaccgctt gcagcaaatg ctgccggaag cgcccgtcgt catctcagaa gggcgctcgt
     5521 ttccggttga acgccgttat ttaccgctgc ccgcgcatca gcgttttgac gaagccgttg
     5581 cggtagccac cgccgaaatg gtgcgtcagg aaagtggatc attactgtta tttttacctg
     5641 gcgtcggaga aattcagcgc gtgcaggaac aactggcttc gcgcatcggc agtgatgtgg
     5701 tgctctgccc gctgtatggc gtgttgtcac tgaacgatca gcgaaaagcg atcctcccgg
     5761 caccgcaagg gatgcgcaaa gtggtgctgg cgaccaatat tgctgaaacc agtttaacca
     5821 tcgaaggtat tcgtctggtg gtggattgtg cccaggagcg tgtggcgcgc tttgatccgc
     5881 gcacggggct tacgcggttg attactcaac gcgttagtca ggcatccatg acgcagcgtg
     5941 ccgggcgcgc cgggcgtctg gagccgggta tcagcctgca tttaatcgcc aaagaacaag
     6001 cagaacgcgc cgcggcgcaa agtgagccgg agatcttaca aagcgatctt tccggtttgc
     6061 tgatggaatt actgcaatgg ggatgcagcg atccggcgca gatgagctgg ctggatcaac
     6121 cgccaacggt gaatctactg gccgcgaaac gcctgttaca aatgctgggg gcgctggagg
     6181 gtgaacggct tagtgcgcaa gggcaaaaaa tggcagcgct gggtaacgat ccgcgtttag
     6241 cggcaatgct ggttaacgca aagagcgatg acgaagctgc taccgcggca aaaattgccg
     6301 ccattctcga agagccgcca cggatgggca atagtgacct gggcgtggcg ttttcgcgca
     6361 atcaaccagc ctggcagcaa cgtagtcagc aactgttaaa acgcttaaac gtacgcggcg
     6421 gtgaggcaga cagttcgctt atcgcgccgc tacttgccgg ggcgtttgcc gatcgcattg
     6481 ctcatcgccg tgggcaagat ggacgctatc aactggcaaa cagcatggga gcgatgctcg
     6541 atgccgacga cgcgctaagc cgccacgaat ggttgatcgc accgttatta ttgcagggca
     6601 gcgcctcgcc ggatgcgcgg attttactgg cgctaccggt cgatattgat gaattagtac
     6661 aacgctgtcc gcagctggtg cagcaatctg acaccgtgga atgggatgac gcgcaaggta
     6721 cgctgaaagc ctggcgtcgg ctacaaatcg gtcagttgac ggtgaaagtg cagccgctgg
     6781 cgaaaccgtc agaagacgag ttgcatcagg cgatgcttaa tggcatccgt gataaaggtt
     6841 taagcgtgct caactggacg gcggaagcgg aacagctacg cttgcgtttg ttatgcgccg
     6901 caaagtggtt gccggaatat gactggccag cggttgatga tgaaagtttg ttggcgacgc
     6961 tggaaacgtg gctgctgcca catatgaccg gcgtacattc actacgcggc ctgaaatcac
     7021 tcgacattta tcaggcacta cgcggattac ttgattgggg aatgcagcaa cgtctggata
     7081 gtgaattgcc tgcgcattac actgtgccga cgggaagccg gatcgccatt cgttatcatg
     7141 aagataaccc gcccgcgctg gcggtgagaa tgcaggagat gtttggtgag gccactaatc
     7201 cgacgatcgc ccaggggcgc gtgccgctgg tgctgaagtt gctttcccct gcccaaaggc
     7261 cgctgcaaat cacgcgtgat ttgggggcct tctggaaagg ggcgtatcgt gaggtgcaaa
     7321 aggagatgaa agggcgttat cccaaacacg tctggccgga cgacccggca aacacggcac
     7381 cgacgcgacg gacgaaaaag tattcgtaag gttggttttc tccctctccc tgtgggagag
     7441 ggccggggtg agggcatcag cgcgcacgtt caccctcacc ctaaccctct ccctcaaggg
     7501 agaggggacc gatcgagcgc aaattttgag agatatcttc ttctgtcttg taacagaaga
     7561 acagaaaatc gggcttttgc gcctgaatat tgcggagaaa aagcatggcc gggaatgacc
     7621 gcgagccaat tggacgcaaa gggaaaccga cgcgtccggt caaacaaaag gtaagccgtc
     7681 gtcgttacga agatgacgat gattacgacg attatgatga ctatgaggat gaagaaccga
     7741 tgccgcgcaa aggtaagggc aaaggcaaag ggcgtaagcc tcgtggcaaa cgcggctggc
     7801 tatggctact gctaaaactg gctatcgttt ttgccgtgct gatcgccatt tacggcgttt
     7861 atctcgatca aaaaattcgt agccgtattg atggcaaggt ctggcaactg cccgcggcag
     7921 tttatggccg aatggtcaac cttgaaccag atatgaccat cagcaagaac gaaatggtga
     7981 agctgctgga ggcaacacag tatcgtcagg tgtcgaaaat gacccgtcct ggcgaattta
     8041 ccgtgcaggc caacagcatt gagatgatcc gccgtccgtt tgatttcccg gacagtaaag
     8101 aagggcaggt gcgtgcgcgt ctgacctttg atggtgatca tctggcgacc atcgtcaata
     8161 tggagaacaa ccgtcagttc ggtttcttcc gtcttgatcc gcgtctgatc accatgatct
     8221 cctcgccaaa cggtgagcag cgtttgtttg tgccgcgcag tggtttccct gatttgctgg
     8281 tggatacttt gctggcgaca gaagaccgtc atttttacga gcatgatgga atcagtctct
     8341 actccattgg gcgtgcggtg ctggcaaacc tgaccgcagg tcgcacggtg cagggtgcga
     8401 gtacgctgac gcaacagctg gtgaaaaacc tgttcctctc cagcgagcgt tcttactggc
     8461 gtaaagcgaa cgaagcttac atggcgctga tcatggacgc gcgttacagc aaagaccgta
     8521 ttcttgagct gtatatgaac gaggtgtatc tcggtcagag cggcgacaac gaaatccgcg
     8581 gcttcccgct ggcaagcttg tattactttg gtcgcccggt agaagagcta agcctcgacc
     8641 agcaggcgct gttagtcggt atggtgaaag gggcgtccat ctacaacccg tggcgtaacc
     8701 cgaaactggc gctggagcga cgtaatctgg tgctgcgtct gctgcaacag caacagatta
     8761 ttgatcaaga actctatgac atgttgagtg cccgtccgct ggggggtcag ccgcgtggtg
     8821 gggtgatctc tcctcagcca gcctttatgc agctggtgcg tcaggagttg caggcaaaac
     8881 tgggcgataa ggtaaaagat ctctccggcg tgaagatctt cactaccttt gactcggtgg
     8941 cccaggacgc ggcagaaaaa gccgccgtgg aaggcattcc ggcactgaag aaacagcgta
     9001 agttgagcga tcttgaaact gcgattgtgg tcgtcgaccg ctttagtggt gaagttcgtg
     9061 cgatggtcgg aggttctgag ccgcagtttg cgggctacaa ccgtgcgatg caggcgcgtc
     9121 gttcgattgg ttcccttgca aaaccagcga cttatctgac ggctttaagc cagccgaaaa
     9181 tctatcgcct gaacacgtgg attgccgatg cgccaatcgc gctgcgtcag ccgaatggcc
     9241 aggtctggtc accgcagaac gatgaccgtc gttatagcga aagcggcaga gtgatgctgg
     9301 tggatgcgtt gacacgttcg atgaacgtgc cgacggtaaa tctggggatg gcgctggggc
     9361 tgcctgcggt tacggagacc tggatcaaac tgggcgtacc gaaagatcag ttgcatccgg
     9421 ttccggcaat gctgctgggg gcgttgaact taacgccaat cgaagtggcg caggcattcc
     9481 agaccatcgc cagcggtggt aaccgtgcac cgctttctgc actgcgttcg gtaatcgcgg
     9541 aagatggtaa ggtgctgtat cagagcttcc cgcaggcaga acgcgctgtt ccggcgcagg
     9601 cggcgtatct gacactatgg accatgcagc aggtggtaca acgtggtacg ggtcgtcagc
     9661 ttggggcgaa atacccgaac ctgcatctgg cagggaaaac agggactacc aacaataacg
     9721 tagatacctg gtttgcaggc attgacggca gcacggtgac catcacctgg gtaggccgtg
     9781 ataacaacca gccgaccaaa ctgtatggag ccagcggggc aatgtcgatt tatcagcgtt
     9841 atctggctaa ccagacgcca acgccgctga atcttgttcc gccagaagat attgcagata
     9901 tgggcgtgga ctacgacggc aactttgttt gcagcggtgg catgcgtgtc ttgccggtct
     9961 ggaccagcga tccgcaatcg ctgtgccagc agagcgagat gcagcagcag ccgtcaggca
    10021 atccgtttga tcagtcttct cagccgcagc aacagccgca acagcaacct gctcagcaag
    10081 agcagaaaga cagcgacggt gtagccggtt ggatcaagga tatgtttggt agtaattaac
    10141 atctaagcgt gaaataccgg atggcgagtt gccatccggt aaaataacat cccatctaag
    10201 atattaaccc tttcttttca tctggttgtt tattaaccct tcaggaacgc tcagattgcg
    10261 taccgcttgc gaacccgcca gcgtttcgaa tattatctta tctttataat aatcattctc
    10321 gtttacgtta ttattcactt tacatcagag atataccaat ggcgcgttcc aaaactgctc
    10381 agccaaaaca ctcactgcgt aaaatcgcag ttgtagtagc cacagcggtt agcggcatgt
    10441 ctgtttatgc acaggcagcg gttgaaccga aagaagacac tatcaccgtt accgctgcac
    10501 ctgcgccgca agaaagcgca tgggggcctg ctgcaactat tgcggcgcgg cagtcagcta
    10561 ccggcactaa aaccgatacg ccgattcaaa aagtgccaca gtctatttct gttgtgaccg
    10621 ccgaagagat ggcgctgcat cagccgaagt cggtaaaaga agcgcttagc tacacgccgg
    10681 gtgtctctgt tggtacgcgt ggcgcatcca acacctatga ccacctgatc attcgcggtt
    10741 ttgcggcaga aggccaaagc caaaataact atctgaatgg cctgaagttg cagggcaact
    10801 tctataacga tgcggtcatt gacccgtata tgctggaacg cgctgaaatt atgcgtggcc
    10861 cggtttccgt gctttacggt aaaagcagtc ctggcggtct gttgaatatg gtcagcaagc
    10921 gtccgaccac cgaaccgctg aaagaagttc agtttaaagc cggtactgac agcctgttcc
    10981 agactggttt tgactttagc gatgcgttgg atgatgacgg tgtttactct tatcgcctga
    11041 ccggtcttgc gcgttctgcc aatgcccagc agaaagggtc agaagagcag cgttatgcta
    11101 ttgcaccggc gttcacctgg cgtccggatg ataaaaccaa tttcaccttc ctttcttact
    11161 tccagaacga gccggaaacc ggttattacg gctggttgcc gaaagaggga accgttgagc
    11221 cgctgccgaa cggtaagcgt ctgccgacag actttaatga aggggcgaag aacaacacct
    11281 attctcgtaa tgagaagatg gtcggctaca gcttcgatca cgaatttaac gacaccttta
    11341 ctgtgcgtca gaacctgcgc tttgctgaaa acaaaacctc gcaaaacagc gtttatggtt
    11401 acggcgtctg ctccgatccg gcgaatgctt acagcaaaca gtgtgcggca ttagcgccag
    11461 cggataaagg ccattatctg gcacgtaaat acgtcgttga tgatgagaag ctgcaaaact
    11521 tctccgttga tacccagttg cagagcaagt ttgccactgg cgatatcgac cacaccctgc
    11581 tgaccggtgt cgactttatg cgtatgcgta atgacatcaa cgcctggttt ggttacgacg
    11641 actctgtgcc actgctcaat ctgtacaatc cggtgaatcc cgatttcgac ttcaatgcca
    11701 aagatccggc aaactccggc ccttaccgca ttctgaataa acagaaacaa acgggcgttt
    11761 atgttcagga tcaggcgcag tgggataaag tgctggtcac cctaggcggt cgttatgact
    11821 gggcagatca agaatctctt aaccgcgttg ctgggacgac cgataaacgt gatgacaaac
    11881 agtttacctg gcgtggtggt gttaactacc tgtttgataa tggtgtaaca ccttacttca
    11941 gctatagcga atcgtttgaa ccttcttcgc aagttgggaa ggatggtaat attttcgcac
    12001 cgtctaaagg taagcagtat gaagtcggcg tgaaatatgt accggaagat cgtccgattg
    12061 tagttactgg tgccgtgtat aatctcacta aaaccaacaa cctgatggcg gaccctgagg
    12121 gttccttctt ctcggttgaa ggtggcgaga tccgcgcacg tggcgtagaa atcgaagcga
    12181 aagcggcgct gtcggcgagt gttaacgtag tcggttctta tacttacacc gatgcggaat
    12241 acaccaccga tactacctat aaaggcaata cgcctgcaca ggtgccaaaa cacatggctt
    12301 cgctgtgggc tgactatacc ttctttgacg gtccgctttc aggtctgacg ctgggcaccg
    12361 gtggtcgtta tactggctcc agctatggtg atccggctaa ctcctttaaa gtgggaagtt
    12421 atacggtcgt ggatgcgtta gtgcgttatg atctggcgcg agtcggcatg gcgggctcca
    12481 acgtggcgct gcatgttaac aacctgttcg atcgtgaata cgtcgccagc tgctttaaca
    12541 cttatggctg cttctggggc gcagaacgtc aggtcgttgc aaccgcaacc tttcgtttct
    12601 aatttctctt ttggggcacg gatttccgtg cccatttcac aagttggctg ttatgcagga
    12661 atacacgaat cattccgata ccacttttgc actgcgtaat atctcctttc gtgtgcccgg
    12721 gcgcacgctt ttgcatccgc tgtcgttaac ctttcctgcc gggaaagtga ccggtctgat
    12781 tggtcacaac ggttctggta aatccactct gctcaaaatg cttggtcgtc atcagcctcc
    12841 gtcagaaggg gagattcttc ttgatgccca gccgctggaa agctggagca gcaaagcgtt
    12901 tgcccgcaaa gtggcttatt tgccgcagca gcttcctccg gcagaaggga tgaccgtgcg
    12961 tgaactggtg gcgattggtc gttacccgtg gcatggcgcg ctggggcgct ttggtgcggc
    13021 agatcgcgaa aaggtcgagg aagctatctc gctggttggc ttaaaaccgc tggcgcatcg
    13081 gctggtcgat agtctctctg gcggcgaacg tcagcgggcg tggatcgcca tgctggtggc
    13141 gcaggatagc cgttgtctgt tgctcgacga accgacttca gcgctggata tcgcccacca
    13201 ggttgatgtg ctggcgctgg tgcaccgttt aagtcaggaa cgtggcctga cggtcattgc
    13261 cgtgttgcac gatatcaata tggcggcacg ctactgtgat tatctggtcg ccctgcgcag
    13321 tggtgaaatg attgctcagg gaacgcctgc ggaaattatg cgcggcgaaa ccctcgaaat
    13381 gatttatggc atcccgatgg gtattttgcc gcatccggcg ggtgctgcac ctgtgagttt
    13441 tgtttattga tgagcggctt acctcttatt tcgcgccgtc gactgttaac ggcgatggcg
    13501 ctttctccgt tgttatggca gatgaatacc gcccacgcgg cggctattga ccccaatcgt
    13561 attgtggcgc tggagtggtt gccggtggaa ttactgctgg cgctcggcat cgtgccttac
    13621 ggcgtggcgg ataccatcaa ctatcgcctg tgggtcagcg aaccaccatt gccggactca
    13681 gtgattgacg tcggtttgcg tacagaacct aaccttgaac tgctgaccga aatgaaaccg
    13741 tcgtttatgg tctggtcggc aggatatggc ccttcatcag aaatgctggc acgtattgcg
    13801 ccgggtcgcg gatttaactt cagtgacggc aaacatccgt tggcgatggc gcgtaaatcg
    13861 ctgacggaaa tggcagattt acttaacctg caaagcgcag cggaaacgca tttagcgcaa
    13921 tacgaagact ttatccgcag catgaaaccc cgctttgtga agcgtggtgc acgcccgtta
    13981 ctgctgacga cgcttatcga tccgcgccat atgctggtct tcggtccaaa cagcttgttc
    14041 caggaaattc tcgatgagta cggcatccca aatgcctggc aaggggaaac caacttctgg
    14101 ggcagtaccg ccgtcagtat cgatcgtctg gcagcgtata aagacgttga tgtgctctgt
    14161 tttgatcacg acaacagcaa agacatggat gcgctaatgg caaccccgct gtggcaggcc
    14221 atgccgtttg tccgtacagg gcgctttcag cgcgtacccg cagtctggtt ttatggtgca
    14281 acgctttcgg caatgcactt tgtgcgtgtt ctggataacg ccatcggagg taaagcgtga
    14341 gtaaacgaat tgcgcttttc ccggcgttat tgctggcgct gttagtgatt gtcgctacgg
    14401 cgctcacctg gatgaacttc tcacaggcgc tgccgcgcag tcagtgggcg caggccgcct
    14461 ggtcgccgga tattgacgtc atcgagcaga tgatttttca ctacagcttg ttgccgcgtc
    14521 tggcgatttc gctgctggtg ggcgcgggcc tggggctggt gggcgtgctg tttcagcaag
    14581 tactgcgtaa cccgctggcg gaaccgacga cgttgggcgt tgcaacaggc gcgcaactgg
    14641 ggattaccgt caccacgctc tgggcgatcc ctagcgcgat ggcgagccag tttgctgcgc
    14701 tggcaggggc ttgcgttgtt ggcttaattg tctttggcgt cgcgtggggg aaacggctgt
    14761 cgccggtaac gctgattttc gcggggttgg tagtgagcct ttattgcggc gcaatcaatc
    14821 agttactggt tatcttccat catgaccagt tgcaaagcat gtttctgtgg agcactggaa
    14881 cgctgacgca aaccgactgg ggcggtgttg agcgtttatg gccgcagttg ctgggcggtg
    14941 tgatgctgac gttgctgcta cttcgtccgt taaccctgat ggggcttgat gatggcgtgg
    15001 cgcgcaatct cgggctggcc ttgtcgcttg cgcgtctggc ggcgctgtcg ctggcgattg
    15061 tcatcagtgc gctgctggtg aacgctgtgg ggattatcgg ctttatcggg ttgttcgcgc
    15121 cgctgctggc aaaaatgctg ggggcgcggc gtctgctgcc acgactgatg ctggcgtcgt
    15181 tgattggtgc gctgatcctc tggctttccg atcaaatcat cctctggctg actcgcgtgt
    15241 ggatggaagt gtccaccggt tcggtcactg cgttgatcgg tgcgccgctg ctactgtggc
    15301 tgctgccgcg tttacgcagc attagcgcgc cggatatgaa ggttaacgat cgtgtcgcga
    15361 ctgaacgcca acatgtgctg gcgtttgccc tcgcgggcgg cgtgctgttg ttgatggctg
    15421 tggtggtggc gctgtcgttt ggtcgtgatg cgcacggctg gacgtgggcg agcggggcgt
    15481 tgctcgagga tttaatgccc tggcgctggc cgcgaattat ggcggcgctg tttgcgggcg
    15541 tcatgctggc ggtagcgggc tgtattattc agcgactgac cggcaacccg atggcaagcc
    15601 cggaagtgct ggggattagc tccggcgcgg cgtttggtgt ggtgttaatg ctgtttctgg
    15661 taccgggtaa tgcctttggc tggttgttac ccgcaggcag tctcggggcg gcggtgacgc
    15721 tgttgatcat tatgatcgcc gccggacgcg gtggattttc cccacaccgc atgttactgg
    15781 cggggatggc gttaagtacc gcgttcacca tgcttttgat gatgttgcag gcaagtggtg
    15841 acccgcgaat ggcgcaagtg ctgacctgga tttccggttc gacctacaac gcgaccgatg
    15901 ctcaggtctg gcgcaccgga attgtgatgg tgattttgct ggcgattact ccgctgtgcc
    15961 gccgctggct gaccatttta ccgctgggtg gtgataccgc gcgggcggta gggatggcgc
    16021 tgacgccgac gcgaattgcg ctgctgctgt tagcggcttg cctgacggcg accgcgacga
    16081 tgactattgg accgttgagt tttgttggtt taatggcacc gcatattgcg cggatgatgg
    16141 gctttcgacg gacgatgcca cacatcgtaa tttcggcgct ggtgggtggt ttactgctgg
    16201 tgttcgctga ctggtgtggg cggatggtgc tgtttccatt ccagatcccg gcggggctgc
    16261 tgtcgacctt tatcggtgcg ccatatttta tctatttgct gagaaagcag agccgttaat
    16321 agaatgcctc gcccggtggg cgaggcaaaa ttatcacaac ttcgcgaaca cccgacgtgc
    16381 agcatcgatg gtgttattga tatcttccat gctgtgcgcc acagacataa agcccgcttc
    16441 aaacgctgac ggtgccaggt aaacaccttc atccagcatc atatggaaga aacgcttaaa
    16501 acgctcgacg tcacaggcca tcacatcctg atagcacgtc acggactcgg cgtcggtaaa
    16561 gaagataccg aacataccac cgacgtggtt aacgaccagc ggaattcccg cttcttctgc
    16621 cgcttccagc agaccttctg ccagacgtgt tgtcagctca tccagcgttt cgtgaatgcc
    16681 cggctgcgcg acttcattca gacaggcgaa acccgctgcc atcgcaatcg ggttaccgga
    16741 aagcgtaccc gcctgataga ccggacctgt cggggccagc gcatccatta catcacgacg
    16801 accaccgaat gcgcctaccg gcattccacc gccgatgatt tttccgaggc aggttaaatc
    16861 tggcactacg gcgaaataat cctgtgcgcc agctagcgct acgcggaaac cggtcatcac
    16921 ttcatcgatg atcagcaacg cgccaaattc gtcgcacagc gcacgcagac ctggcaggaa
    16981 ctctggcagc ggcggaacac agttcatatt gcctgccacc ggctcgacga taatacaggc
    17041 aatctcttgc gggtattgct caaacgcggc gcgtacagag gccagatcgt tataagtaca
    17101 ggttaaggta tgtttggcga aatctgccgg aacgcccggc gagtttggct ggcctaacgt
    17161 gagtgcgcca gaaccggctt tcaccagcag gcagtcagcg tgaccgtggt aacaaccttc
    17221 aaatttaatg attttgtcgc gaccggtaaa accacgggcc aggcggatgg cgctcatcgt
    17281 cgcctcggtg ccggagttca ccatgcgcac catatccatg gtcgggacca gttcggtcac
    17341 cagttgcgcc attttcactt ccatttcggt cggtgcacca aagcttaaac cacgctcggc
    17401 ggcttcaatc acggcattgc ggatcgccgg atggttatgg cccagcacca tcggccccca
    17461 ggaaccgaca taatcgatat aggctttgcc atcaacatcg tacagataag cgccgtccgc
    17521 tttttcgata aacagtggag tgccgcccac gccagtaaag gcgcgaacag gggagttcac
    17581 accgccaggg atcagctcgc gcgctgcgct gtaaagattt tcagacttac tcatggaggg
    17641 ttcctgattc gtagaaaaag tgaatggctg ctattccatg ttattcataa caagttaaat
    17701 actcgtcaaa catcaggctg cttgtactgg tgcaatcctg atttcttaga gtataaaagt
    17761 tttgtgcatt tgaaacatta cgctttgcaa aggattttca tggaacgtgc gagtaaaatg
    17821 ccgtcatctt atttgtatga ccaataagtg atcattggat gaaaactgat actccctctt
    17881 tagaaacacc gcaggccgcg cgcctgcgac gcagacaact gattcgccaa cttcttgagc
    17941 gcgataaaac cccgttagcc attttgttta tggcggcagt cgtcggcacg cttgtcgggc
    18001 tggctgcggt tgcttttgac aaaggtgtcg cctggttgca gaaccaacgt atgggggcgc
    18061 tggtacatac tgctgataat tatccgctac tgttaaccgt cgcttttctc tgttcggcgg
    18121 tgctggcgat gttcggctac tttttggtgc gcaaatacgc gcccgaagca gggggatcgg
    18181 ggatcccgga aattgaaggg gcgctggaag atcaacgtcc tgttcgctgg tggcgtgtat
    18241 tgccggtgaa attctttggc gggctgggga cactcggcgg aggcatgggg ctagggcgcg
    18301 aaggaccaac cgtgcagatc ggcggcaata tcagtcgcat ggtgcttgat attttccgcc
    18361 tgaaaggtga tgaagctcgc catacgctgc tggcaaccgg tgctgctgcg gggctggctg
    18421 cggcctttaa cgcgccgctg gcgggtattt tgtttattat cgaagagatg cgtccgcagt
    18481 ttcgctatac gttaatttcg attaaagcgg tatttattgg cgtcattatg tcgaccatta
    18541 tgtaccggat ttttaatcat gaagttgcgt tgattgacgt cggtaaactt tctgatgcgc
    18601 cgcttaatac gctgtggctt tatctgatcc tcggtattat ttttggcatt ttcggcccta
    18661 tttttaataa atgggtgctg gggatgcagg atttgctgca ccgtgtgcac ggcggcaata
    18721 ttaccaaatg ggtactgatg gggggtgcga ttggcggtct gtgtggattg ctggggtttg
    18781 tggcaccagc aacgtcgggc ggcggtttta acctgattcc tatcgctacc gcggggaatt
    18841 tcagcatggg aatgctggtg tttatcttcg tcgctcgggt cattaccacc ttactctgct
    18901 tctcttccgg cgcgccgggc ggtatttttg ccccgatgct ggcgctgggt actgtgctgg
    18961 gaaccgcttt cggaatggtt gccgttgagc tgtttccgca atatcacctg gaggcgggga
    19021 cgtttgctat tgccggaatg ggggcattac tggcggcatc tattcgcgcg ccgttaacgg
    19081 ggatcattct ggttctggag atgaccgata actaccagct cattttgcca atgattatta
    19141 ccggtcttgg cgcaacacta ttagcgcaat ttaccggcgg gaaacctcta tactcggcga
    19201 ttcttgcgcg cacgctggca aaacaggaag ctgagcaact ggcgcgaagc aaggccgcat
    19261 cagccagcga gaatacttga acgaaatacc agggtattag ataatggcga ttattattgg
    19321 gttagaattt gcccgattgc cgatgtcgtt tggagcaaaa tatgagtgat gacgtagcac
    19381 tgccgctgga gtttaccgac gcagcagcca acaaagttaa aagcctgatc gctgacgaag
    19441 ataacccgaa tctgaaatta cgcgtgtata tcaccggtgg cggttgcagc ggcttccagt
    19501 atggtttcac ctttgatgat caggtgaacg aaggcgatat gaccatcgaa aaacagggcg
    19561 ttggcctggt ggttgatccg atgagcctgc aatatctggt cggcggttcc gttgactaca
    19621 ccgaaggact ggaaggttct cgtttcatcg tgaccaaccc gaacgcgaaa agcacctgcg
    19681 gttgcggttc ttcctttagc atctaatctg ttgttcatgt gccggataaa ctatccggca
    19741 catcttctct cttcaacgcc cattctcatc cagcgcaaac gtcggtagct taagatgcca
    19801 acgaatagcc gccagccgaa tcaatagcgt cacgaccatg cccatcatac tggctgtttc
    19861 cagtggtacg gaaaatgtgt aataagccgt agcgtggaca ataccgccga taatacaggc
    19921 agttgcgtag atttctgtac gtaaaatcat ggggatttcg cggaccagta catcacgaat
    19981 gatcccgccg ccaacgccag taatgacgcc catacaaacc gcgattaacg gaccggcttc
    20041 cgcattaaag gctttattca cgccaatgcc gacaaacacc gccagaccaa cggcgtccag
    20101 caccggcaac atccattttg gtaagcgtct tggctggcgc accagcacga tggtcagcat
    20161 gctggtgacc atggcaacga ccagatcggt gggatctttc acccaaaata ccgggccgtg
    20221 atccagcgcc atgtcgcgaa ttgttccgtc gcctactgcg gtaaccacgc ccagtaccag
    20281 aacaccaaaa gggtccatac gcaattttcc ggctaacaaa acgccgtaga tggcaaatac
    20341 cgctgtgccg actatatcca gccaatagac gagcattgtt caatccccac tgagcacctg
    20401 aaaaggtcag gcactaatct acctgtgaaa gcgcattaca gagctgttgt gctgcgagga
    20461 taatacgtgg gcttgcacgt tcaaaccagt cactcgtgag aggaataacg ggaattttga
    20521 gctgttcacc ccagtattgt ttgattttag gaatttggtc cggtccgcct gtaatgacaa
    20581 tagcctgtgg cgagcgtgct aacacctgtt cgcggctaac ttgcggccag ggaacccggc
    20641 tgtctttaaa gatgttttct ccgccacaaa cttcgagtac ctggttctga atcgactctt
    20701 ttccactggt aaatggcgga ttaatgccga attgcagaaa aacacgtttt ttaggtttat
    20761 cagcatattg cgctttcaat tgcacgtact gatccagcag ggattgcgcg gcttgttcgg
    20821 ccttgtctgg ttgcggactc cagggggcca gttgacgtaa cgcattggca atttgttcaa
    20881 tgcttgtcgc atcgacccac atcactttta ttcccagcga agccagctgg tcaacctgcc
    20941 gctcggcatt acctccacgc caggcaatca ccagatcggg tttcagcgcg acaatgcgtt
    21001 ccagattcat cccctgccag gtggaaacct gctcaatctt ttgcgcttgt ggaggatagt
    21061 cggaatagct gctgacccca accggcgtga tcccggcggc aaaggcaagt tcagtgttgg
    21121 cgggagaaag cgtgatgacg cgcggcgcgg cgttgagcca cagtggcgcg agaaaagaca
    21181 gggcgaccag cgccctgaac agtgatttag ccatgtgcca gtttctgcac cagtgactca
    21241 accatcaggc tggactgttt agcggcaaca gccaggaact catcgaagct aagatgagac
    21301 tgttgatcgg ccacgtcgga gatggcgcgt acgacaacaa acgggacgtt gaaattgtgg
    21361 cagacatggg cgattgccgt cgcttccatc tctacagcaa tggcctgtgg gaagttgtgg
    21421 cggattttcg ccagaccaac agaaccgttg atgaaagcgt cgccgctaac aatcaggcca
    21481 cgtacagcgt taagattcag ttcggcaatg caggcctcag cggcagcgat cagtttatcg
    21541 tcagctttaa agcctgccgg acagcctggt aactgaccgt attcataacc aaatgccgtg
    21601 acatccgcgt cgtgataacg cgcttcgtcc gagacaacga tatcgcccac tttcagcgtt
    21661 ggtgccaggc caccggcaga accggtgtta ataatcacat ctggcttgca gtgttccaac
    21721 agcagagtgg cacccagcgc cgcagcgact ttaccgatgc ccgatttcag aagcgcaacc
    21781 tcggttccat tcagttggcc ggtatagatt tcgcaaccgc cgagactgat agtttgacgg
    21841 ttttcgattt tgtcacgcag cagcgtaact tcttcttcca ttgcaccaat gatgccgatt
    21901 ttcatagatt tactcgcgat aagcccgatt tgaaggcata gtttaccatg cgcttacggg
    21961 gaagcgtatt tctcacgcgg gagaggacat ggcacagatt gatttccgaa aaaaaataaa
    22021 ctggcatcgt cgttaccgtt caccgcaggg cgttaaaacc gaacatgaga tcctgcggat
    22081 cttcgagagc gatcgcgggc gtatcatcaa ctctccggca attcgtcgtc tgcaacaaaa
    22141 gacccaggtt tttccactgg agcgcaatgc cgccgtgcgc acgcgtctta cccactcgat
    22201 ggaagtccag caggtgggac gctacatcgc caaagaaatt ttaagccgtc tgaaagagct
    22261 taaattgctg gaagcatacg gcctggatga actgaccggc ccctttgaaa gcattgttga
    22321 gatgtcatgc ctgatgcacg atatcggcaa tccgccgttt ggtcattttg gcgaagcagc
    22381 gataaatgac tggtttcgcc aacgtttgca cccggaagat gccgaaagcc agcctctgac
    22441 tgacgatcgc tgcagcgtgg cggcactacg tttacgggac ggggaagaac cgcttaacga
    22501 gctgcggcgc aagattcgtc aggacttatg tcattttgag gggaatgcac aaggcattcg
    22561 tctggtgcat acattgatgc ggatgaatct cacttgggca caggttggcg gtattttaaa
    22621 atatacccgt ccggcgtggt ggcgtggcga aacgcctgag acacatcact atttaatgaa
    22681 aaagccgggt tattatcttt ctgaagaagc ctatattgcc cgcttgcgta aagaacttaa
    22741 tttggcgctt tacagtcgtt ttccattaac gtggattatg gaagctgccg acgacatctc
    22801 ctattgtgtg gcagaccttg aagatgcggt agagaaaaga atatttaccg ttgagcagct
    22861 ttatcatcat ttgcacgaag cgtggggcca gcatgagaaa ggttcgctct tttcgctggt
    22921 ggttgaaaat gcctgggaaa aatcacgctc aaatagttta agccgcagta cggaagatca
    22981 gttttttatg tatttacggg taaacaccct aaataaactg gtaccctacg cggcacaacg
    23041 atttattgat aatctgcctg cgattttcgc cggaacgttt aatcatgcat tattggaaga
    23101 tgccagcgaa tgcagcgatc ttcttaagct atataaaaat gtcgctgtaa aacatgtgtt
    23161 tagccatccg gatgtcgagc agcttgaatt gcagggctat cgggtcatta gcggattatt
    23221 agagatttat cgtcctttat taagcctgtc gttatcagac tttactgaac tggtagaaaa
    23281 agaacgggtg aaacgtttcc ctattgagtc gcgcttattc cacaaactct cgacgcgcca
    23341 tcggctggcc tatgtcgagg ctgtcagtaa attaccgtca gattctcctg agtttccgct
    23401 atgggaatat tattaccgtt gccgcctgct gcaggattat atcagcggta tgaccgacct
    23461 ctatgcgtgg gatgaatacc gacgtctgat ggccgtagaa caataaccag gcttttgtaa
    23521 agacgaacaa taaattttta ccttttgcag aaactttagt tcggaacttc gggctataaa
    23581 acgaatctga agaacacagc aattttgcgt tatctgttaa tcgagactga aatacatgaa
    23641 aaaaaccaca ttagcactga gtgcactggc tctgagttta ggtttggcgt tatctccgct
    23701 ctctgcaacg gcggctgaga cttcttcagc aacgacagcc cagcagatgc caagccttgc
    23761 accgatgctc gaaaaggtga tgccttcagt ggtcagcatt aacgtagaag gtagcacaac
    23821 cgttaatacg ccgcgtatgc cgcgtaattt ccagcagttc ttcggtgatg attctccgtt
    23881 ctgccaggaa ggttctccgt tccagagctc tccgttctgc caggggggcc agggcggtaa
    23941 tggcggcggc cagcaacaga aattcatggc gctgggttcc ggcgtcatca ttgatgccga
    24001 taaaggctat gtcgtcacca acaaccacgt tgttgataac gcgacggtga ttaaagtcca
    24061 gctgagcgat ggtcgtaagt tcgacgcgaa gatggttggc aaagatccgc gctctgatat
    24121 cgcgctgatc caaatccaga acccgaaaaa actgaccgca attaagatgg cggattctga
    24181 tgcactgcgc gtgggtgatt acaccgtagc gattggtaac ccgtttggtc tgggcgagac
    24241 ggtaacttcc gggattgtct ctgcgctggg gcgtagcggc ctgaatgctg aaaactacga
    24301 aaacttcatc cagaccgatg cagcgatcaa ccgtggtaac tccggtggtg cgctggttaa
    24361 cctgaacggc gaactgatcg gtatcaacac cgcgatcctc gcaccggacg gcggcaacat
    24421 cggtatcggt tttgctatcc cgagtaacat ggtgaaaaac ctgacctcgc agatggtgga
    24481 atacggccag gtgaaacgcg gtgagctggg tattatgggg actgagctga attccgaact
    24541 ggcgaaagcg atgaaagttg acgcccagcg cggtgctttc gtaagccagg ttctgcctaa
    24601 ttcctccgct gcaaaagcgg gcattaaagc gggtgatgtg atcacctcac tgaacggtaa
    24661 gccgatcagc agctttgccg cactgcgtgc tcaggtgggt actatgccgg tgggcagcaa
    24721 actgaccctg ggcttactgc gcgacggtaa gcaggttaac gtgaacctgg aactgcagca
    24781 gagcagccag aatcaggttg attccagctc catcttcaac ggcattgaag gcgctgagat
    24841 gagcaacaaa ggcaaagatc agggcgtggt agtgaacaac gtgaagacgg gcactccggc
    24901 tgcgcagatc ggcctgaaga aaggtgatgt gattattggt gcgaaccagc aggcagtgaa
    24961 aaacatcgct gaactgcgta aagttctcga cagcaaaccg tctgtgctgg cactgaacat
    25021 tcagcgcggc gacagcacca tctacctgtt aatgcagtaa tctccctcaa ccccttcctg
    25081 aatacgggaa ggggttctcc ttacaatctg tgaacttcgc cacaactcca tacatcttca
    25141 tcatcctttg ggcatttgca caatgccgta cgttacgtac ttccttatgc taagccgtgc
    25201 ataacggagg acttatggct ggctggcatc ttgataccaa aatggcgcag gatatcgtgg
    25261 cacgtaccat gcgcatcatc gataccaata tcaacgtaat ggatgcccgc gggcgaatta
    25321 tcggcagcgg cgatcgtgag cgtattggtg aattgcacga aggtgcattg ctggtacttt
    25381 cacagggacg agtcgtcgat atcgatgacg cggtagcacg tcatctgcac ggtgtgcggc
    25441 aggggattaa tctaccgtta cggctggaag gtgaaattgt cggcgtaatt ggcctgacag
    25501 gtgaaccaga gaatctgcgt aaatatggcg aactggtctg catgacggct gaaatgatgc
    25561 tggaacagtc gcggttgatg cacttgttgg cgcaggatag ccgtttgcgg gaagaactgg
    25621 tgatgaacct gattcaggca gaggagaata ctcccgcact tctgaatggg cgcaacggct
    25681 ggggatcgat ctcaatcaac cgcgagtggt ggctattgtt gaggtcgaca gcggtcagct
    25741 tggcgtggac agcgcaatgg cggagttaca acaactgcaa aacgcgctga ctacgcccga
    25801 gcgtaataat ctggtggcga ttgtctcgct aaccgaaatg gtggtgttga aaccggcgtt
    25861 gaactctttt gggcgctggg atgcagaaga tcatcgtaag cgagttgaac aactgattac
    25921 ccgcatgaaa gagtacggcc agctgcgttt tcgcgtttca ctgggcaact attttaccgg
    25981 tcctggcagt attgcccgat cctatcgtac ggcgaaaacg acgatggtgg tgggtaaaca
    26041 gcggatgcca gaaagtcgct gctattttta tcaggatctg atgttacctg tgttactcga
    26101 cagtttgcgt ggcgactggc aggccaacga actggcgcga ccgctggcgc ggctgaaagc
    26161 gatggacaat aacggcttgc tgcgacgaac gctggcggcg tggtttcgcc acaatgtgca
    26221 accgctggca acgtcaaagg cgttgtttat tcatcgtaat accctggagt atcggcttaa
    26281 tcgtatatcg gaactgaccg ggcttgattt gggcaatttt gatgacaggt tgctgctgta
    26341 tgtggcgtta caactggatg aagagcggta gattatgcgt taaggttgag gcagcgctgg
    26401 ctcattcccc tcagcccgaa ggggagaggg gaatgcccga gttaaccgtc aattcgggat
    26461 tatttacgcg ttagtttttc cagatcggct tcaatctcgc tgatcttatt ggttacgact
    26521 gactccaggt gacgtaagtc gtcgaggatc ttacgcttaa gatcaacttc gctgcggtca
    26581 cgctgacaga tttgatcaag ctcatcaatg atataccgta gattcgggct gatttcctgg
    26641 acttctttat aaccctgacc cacaccatca gcgacgaccg ttttacgctg acgcggatat
    26701 ttaaacttaa cgctcttggc gaaaaactcg cctttgtctt tctggaaata gattttcaga
    26761 atatcgttgt tggcttcctg ccggaggctg taacgatcaa tttcttcagg attggtaata
    26821 cccagacttt tcagattgtc gtacatagcg ttacctcaaa atgagtcagt aaattgtgct
    26881 tattttagca tttggcttgg cccgcccggc tcgactgtga tcgcagaaag cctttttctt
    26941 ccagacttct atcatggcgt aaaaatcaaa aaattacctg ctttattgtg gcgatattcc
    27001 atgggatgga ttagcacatt tatcttcgtt ttgcgaatta catgacagtc cgaaacaaga
    27061 tgaaaaagta tttaaaaaag ggggaggaag aatgagtagg acgaattatt ctgagccaga
    27121 aagcatacgg gcagcatcat gctgcccgta tatgtactct taatcgatgg tacgcagcag
    27181 ttcgttaatg ccgactttgc cgcgagtttt cgcgtcaact ttcttaacga taaccgcaca
    27241 gtagaggctg tatttgccat cttttgacgg cagattacct gaaacaacca cagaccccgc
    27301 cggaacgcga ccgtagtgga tttcgccggt ttcacggtcg taaatacggg tgctctgacc
    27361 aatgtatacg cccatagaga tgacggaacc ttcttcgaca atcacccctt caaccacttc
    27421 agagcgtgcg ccaatgaagc agttatcttc aataatggtc gggttagcct gcagcggctc
    27481 cagcacgccg ccgatgccca cgccaccgga aaggtggacg tttttaccaa tctgcgcaca
    27541 agaaccgacg gtcgcccagg tatcaaccat ggtgccttca tcaacatatg cgccgatgtt
    27601 aacgtaagac ggcatcagca cggtgttacg ggcaataaac gcaccctgac gtaccgccgc
    27661 tggtggcaca acgcggaagc cttctttctg gaaacgtgct tcgtcgtagt cggcgaattt
    27721 catcggcact ttgtcgaagt agcggctttc tgccccttcg atcacctgat tatcattaat
    27781 acggaaagag agcagcaccg cttttttcaa ccactgatgc gtcacccact gaccgtcaat
    27841 tttttccgct acacgcagtg cgccggaatc cagcagggcg atcacctgat ttaccgcttc
    27901 gcgggtaacg gtgtctgcat tggctggcgt gatctcggca cggcgttcaa aagcggtttc
    27961 aataatgttc tgtaactgct gcattgttaa actcttttca tatcagtaaa cacatcaccc
    28021 tttatcgttt ggattgaggg cctctgtcaa ccgctgatgc acttcctgct gcaactcgtt
    28081 attaagcgca cgccggtcag cggtggcaat tatgaataaa tcttctactc gctcgccaat
    28141 ggttgtaatt cgggcaccat gaagcgaaat tcccagatcg gcaaaaattt tcccgactcg
    28201 cgccagcagt ccaggttggt cgagggcgat cagttcgagg aacgatttgc ggtcggtatg
    28261 ggtcggcaaa aacgttactt cggtttcaac agtaaaatgg cgtaatttgg cgggttggcg
    28321 acggggctgc ggtggctgcc agctactttg cgtcagcacc tgctccagac caaaccgaat
    28381 aacctcatgg cgatctgcgg acagcgggct gccatcgggt tccagcacga taaaggtatc
    28441 catcgccata ccgtcgcgag tggtgaaaat ttgtgcgtcg tgaacactta aattgcggcg
    28501 gtctaattcg gcacagacgg cggcaaacag ataggggcgg tccgggctcc agataaaaat
    28561 ctcggtgcct ccacgcgtag cctgcgggct aagcaatacc agcggtttgc ttaaatcatg
    28621 ctgtaataaa tggcgggcat gccaggccag ttgatttggg ctatggcgga caaaatagtt
    28681 agcacgacag cgtgaccaaa tttggtgcag cgcctcttcg tcgatgttat ccatgcgcag
    28741 tagtgccagt gcctggagtt ggtgatggcg aacccgttcg cgcatatccg gcgtgttttg
    28801 cattccgcgt cgtagctgct tttcggtggc aaaatagagt tcgcgcaaca gactttgctt
    28861 ccagctattc cacagtgttt cgttggtggc gcaaatgtca gccactgtca ggcataccag
    28921 atagcgcaga cgattttccg tttgaacttc ttcggcaaac tgcttgatga cttcagggtc
    28981 ctgaatatcg cggcgttgtg ctgtcaccga catcagcaag tgctggcgaa ccagccaggc
    29041 aaccagctgt gtttcgcgtg agttcagccc gtggagttct gcaaaatgca ctacatcctg
    29101 agcaccgaga atggagtggt cgccgccgcg tcctttggcg atatcgtgaa acagcgcggc
    29161 gatgaaaatc agctcagttg acggcaggcg cggccagacg tccacacaca acggatggcg
    29221 ctggcgcgtt tcttcactgg caaaactctc cagtttcagc atcacgcgga tagtatgttc
    29281 atccaccgtg taggcgtgga acagatcaaa ctgcatctgc ccgacgatat gcgaccattg
    29341 cggcatatac gcgccgagca cgctatggcg atgcattggc aatagtccgc gccgcaccgc
    29401 tccggggtga cgcagaatgc tcaaaaacag ttttcgtgct tgcggaatat tacacagcgg
    29461 ttgttgcaga tggcgacggg catggcgtaa ctggcgcagc gtggtggagt aaatgccggt
    29521 gatcgcactg ttgcgcacca tggtgtagaa catacgcaag atggcttccg gctggcgcat
    29581 aaatagtgtt tcatcacgca ggtcgattag cgtaccgcgt agctgaaact catcgtcgag
    29641 tggacgtggt ttttcgtcgg cgggaagggc gaggatggct tcatcgaaca gttgcagcag
    29701 catctggttg agttcactga cgcggcgtgt aacgcggaag taatccttca tcatccgctc
    29761 gaccggttcg ttgccttcac cactgtaatt cagacgctga gcgacgctaa gttggcgatc
    29821 gaataacaga cgattatcgt aacggctgac gaccagatgc agggcaaagc gaatacgcca
    29881 cagtatatgc agacattcgt ttaattccgc ccgctccgct gaggttaaga agccaaaccc
    29941 gaccatttca tccagcgatg ttgcgccaaa atgacggcgg gccacccatt gcagagtgtg
    30001 aatatcgcgc aagccgccag ggctactttt gatgtctggt tcaaggttgt agctggtgcc
    30061 atggtaacgc tgatggcgct ggttctgttc ttcaactttc gctgcgtaga acttgtcgga
    30121 aggccagaat ccttcgctaa aaatatgttt ttgcagttcg aggaagagcg caacatcgcc
    30181 aattaataag cgggattcga ttaaattggt ggcgacggtt aaatccgata acccttccaa
    30241 catgcactct tcaagcgtgc gcacactatg accgacttcc agctttacat cccagagcag
    30301 cgttaacagc tcgcccactt tttgcgcctg atcgtccggg agctttttac ggcttaaaat
    30361 cagtaaatcg atgtcagaaa gtggatgcag ctcgccacga ccgtagccac cgacggcaac
    30421 caatgccagg tcggcaatct ggctgaatcc cgcttcaatc cataatcgtt gcaggagctg
    30481 gtctatgaac tcggtacgtg cctcaatcaa ctgttctgca gagatcccat tgtcaaaggc
    30541 atcacccagc caacgctgga aagtatcgat atgggctttt atcccacaga cggttaattc
    30601 atcacggggc caggcgcatg gattttgcgg ttgaccgggc agggtggaga gagcggtgtt
    30661 tgcgtactgt tctggaaggg tattcattgt gcgccaccca taagattaaa ttatcgcatt
    30721 aaaaaagccg gcattcgccg gcttcatctt attcgtcgtg cgagattatc gccgggatgg
    30781 tgtcatcctt gcgtagcgtc aaaatttcgc agccgttatc agtcaccaca atagtatgct
    30841 catattgtgc agacaagctg cgatctttgg tttttaccgt ccagccatct ttcatggtgc
    30901 ggatctcttt tttacccgcg ttgaccattg gctcaatggt gaacgtcatc cccggtttca
    30961 gcacgacgtt ggtttcacgg gagtcatagt gcagcacctg cggttcttca tggaagccgc
    31021 gaccaatacc gtgtccgcaa tattcacgaa cgacggagaa gccttctgct tcgacaaatt
    31081 tctgaatcgc cgcaccgatt tcgcgcagat taatgcctgg ttttaccatg cgtagcgcca
    31141 ggtacaggct ttcttgcgtg atgcggcaca ggcgttcgcc catgatggtc ggcttaccga
    31201 cgataaacat tttcgaggta tcgccgtgga aaccatcttt gattacggtg acatcaatgt
    31261 taacgatatc gccatctttc agcagcttag catcgtccgg gataccgtgg cacaccactt
    31321 cattaataga gatgcaaacg gatttcggat agccgtgata gccgaggcag gcagaaaccg
    31381 cgtgttgttc attaacaatg taatcattac agatgcgatc cagctcgccg gtgctgacgc
    31441 ccggtttaac atacggttcg atcatctcca gcacttcggc agccagtcgg ccagcgacgc
    31501 gcattttttc gatatcttct ggggtcttga ttgagatagc cattaattct gtccatcagc
    31561 gtcggtgata ccgacaatat atatgtaagt gccgtcaatg gtatcacacc cgggcaaatt
    31621 gagaatcatt ctgaatttcg ccaaacgtgc cactgaaggt tttctataat agaaaattcg
    31681 acgtctgatg ctgtacacag cgccaacaat tattggtgtc cacgacgtat ttgtggtata
    31741 aagcgcgccg gacttccgat ccatttcgta tacacagact ggacggaagc gacaatctca
    31801 ctttgtgtaa caacacacac gtatcggcac atattccggg gtgccctttg gggtcggtaa
    31861 tatgggatac gtggaggcat aaccccaact tttatataga ggttttaatc atggcaactg
    31921 tttccatgcg cgacatgctc aaggctggtg ttcacttcgg tcaccagacc cgttactgga
    31981 acccgaaaat gaagccgttc atcttcggtg cgcgtaacaa agttcacatc atcaaccttg
    32041 agaaaactgt accgatgttc aacgaagctc tggctgaact gaacaagatt gtttctcgca
    32101 aaggtaaaat ccttttcgtt ggtactaaac gcgctgcaag cgaagcggtg aaagacgctg
    32161 ctctgagctg cgaccagttc ttcgtgaacc atcgctggct gggcggtatg ctgactaact
    32221 ggaaaaccgt tcgtcagtcc atcaaacgtc tgaaagacct ggaaactcag tctcaggacg
    32281 gtactttcga caagctgacc aagaaagaag cgctgatgcg cactcgtgag ctggagaaac
    32341 tggaaaacag cctgggcggt atcaaagaca tgggcggtct gccggacgct ctgtttgtaa
    32401 tcgatgctga ccacgaacac attgctatca aagaagcaaa caacttgggt attccggtat
    32461 ttgctatcgt tgataccaac tctgatccgg acggtgttga cttcgttatc ccgggtaacg
    32521 acgacgcaat ccgtgctgtg accctgtacc tgggcgctgt tgctgcaacc gtacgtgaag
    32581 gccgttctca ggatctggct tcccaggcgg aagaaagctt cgtagaagct gagtaataag
    32641 gcttgataac tcccccaaaa tagttcgagt tgcagaaagg cggcaagctc gagaattccc
    32701 gggagcttac atcagtaagt gaccgggatg agcgagcgaa gacaacgcat ctgcggcgcg
    32761 aaatatgaag ggggagagcc cttatagacc aggtagtaca cgtttggtta gggggcctgc
    32821 atatggcccc ctttttcact tttatatctg tgtggtttaa tgccgggcag atcacatctc
    32881 cgaggatttt agaatggctg aaattaccgc atccctggta aaagagctgc gtgagcgtac
    32941 tggcgcaggc atgatggatt gcaaaaaagc actgactgaa gctaacggcg acatcgagct
    33001 ggcaatcgaa aacatgcgta agtccggtgc tattaaagca gcgaaaaaag caggcaacgt
    33061 tgctgctgac ggcgtgatca aaaccaaaat cgacggcaac tacggcatca ttctggaagt
    33121 taactgccag actgacttcg ttgcaaaaga cgctggtttc caggcgttcg cagacaaagt
    33181 tctggacgca gctgttgctg gcaaaatcac tgacgttgaa gttctgaaag cacagttcga
    33241 agaagaacgt gttgcgctgg tagcgaaaat tggtgaaaac atcaacattc gccgcgttgc
    33301 tgcgctggaa ggcgacgttc tgggttctta tcagcacggt gcgcgtatcg gcgttctggt
    33361 tgctgctaaa ggcgctgacg aagagctggt taaacacatc gctatgcacg ttgctgcaag
    33421 caagccagaa ttcatcaaac cagaagacgt atccgctgaa gtggtagaaa aagaatacca
    33481 ggtacagctg gatatcgcga tgcagtctgg taagccgaaa gaaatcgcag agaaaatggt
    33541 tgaaggccgc atgaagaaat tcaccggcga agtttctctg accggtcagc cgttcgttat
    33601 ggaaccaagc aaaactgttg gtcagctgct gaaagagcat aacgctgaag tgactggctt
    33661 catccgtttc gaagtgggtg aaggcatcga gaaagttgag actgactttg cagcagaagt
    33721 tgctgcgatg tccaagcagt cttaattatc aaaaaggagc cgcctgaggg cggcttcttt
    33781 ttgtgcccat cttgtaaatt cagctaaccc ttgtggggct gcgctgaaaa gcgacgtaca
    33841 atgtcgctag tattaattca tttcaatcgt tgacagtctc aggaaagaaa catggctacc
    33901 aatgcaaaac ccgtctataa acgcattctg cttaagttga gtggcgaagc tctgcagggc
    33961 actgaaggct tcggtattga tgcaagcata ctggatcgta tggctcagga aatcaaagaa
    34021 ctggttgaac tgggtattca ggttggtgtg gtgattggtg ggggtaacct gttccgtggc
    34081 gctggtctgg cgaaagcggg tatgaaccgc gttgtgggcg accacatggg gatgctggcg
    34141 accgtaatga acggcttggc aatgcgtgat gcactgcacc gcgcctatgt gaacgctcgt
    34201 ctgatgtccg ctattccatt gaatggcgtg tgcgatagct acagctgggc agaagctatc
    34261 agcctgttgc gcaacaaccg tgtggtgatc ctctccgccg gtacaggtaa cccgttcttt
    34321 accaccgact cagcagcttg cctgcgtggt atcgaaattg aagccgatgt ggtgctgaaa
    34381 gcaaccaaag ttgacggcgt gtttaccgct gatccggcga aagattcaac cgcaaccatg
    34441 tacgagcaac tgacttacag cgaagtgctg gaaaaagagc tgaaagtcat ggacctggcg
    34501 gccttcacgc tggctcgtga ccataaatta ccgattcgtg ttttcaatat gaacaaaccg
    34561 ggtgcgctgc gccgtgtggt aatgggtgaa aaagaaggga ctttaatcac ggaataattc
    34621 ccgtgatgga taaataaggg taagattccg cgtaagtatc gcgggggcgt aagtctggtt
    34681 ataaggcgtt attgttgcag gcagtttggt cacggccagc gcgcagcaac cggagcgtac
    34741 aaaagtacgt gaggatggcg agcactgctc ggggccaaaa tggcaaataa aatagcctaa
    34801 taatccagac gattacccgt aatatgttta atcagggcta tacttagcac acttccactg
    34861 tgtgtgactg tctggtctga ctgagacaag ttttcaagga ttcgtaacgt gattagcgat
    34921 atcagaaaag atgctgaagt acgcatggac aaatgcgtag aagcgttcaa aacccaaatc
    34981 agcaaaatac gcacgggtcg tgcttctccc agcctgctgg atggcattgt cgtggaatat
    35041 tacggcacgc cgacgccgct gcgtcagctg gcaagcgtaa cggtagaaga ttcccgtact
    35101 ctgaaaatca acgtgtttga tcgttcaatg tctccggccg ttgaaaaagc gattatggcc
    35161 tccgatctcg gcctgaaccc gaactctgcg ggtagcgaca tccgtgttcc gctgccgccg
    35221 ctgacggaag aacgtcgtaa agatctgacc aaaatcgttc gtggtgaagc agaacaagcg
    35281 cgtgttgcag tacgtaatgt gcgtcgtgac gcgaacgaca aagtgaaagc actgttgaaa
    35341 gataaagaga tcagcgaaga cgacgatcgc cgttctcagg acgatgtaca gaaactgact
    35401 gatgctgcaa tcaagaaaat tgaagcggcg ctggcagaca aagaagcaga actgatgcag
    35461 ttctgatttc ttgaacgaca aaaacgccgc tcagtagatc cttgcggatc ggctggcggc
    35521 gttttgcttt ttattctgtc tcaactctgg atgtttcatg aagcaactca ccattctggg
    35581 ctcgaccggc tcgattggtt gcagcacgct ggacgtggtg cgccataatc ccgaacactt
    35641 ccgcgtagtt gcgctggtgg caggcaaaaa tgtcactcgc atggtagaac agtgcctgga
    35701 attctctccc cgctatgccg taatggacga tgaagcgagt gcgaaacttc ttaaaacgat
    35761 gctacagcaa caggggagcc gcaccgaagt cttaagtggg caacaagccg cttgcgatat
    35821 ggcagcgctt gaggaggttg atcaggtgat ggcagccatt gttggtgctg ctgggctgtt
    35881 acctacgctt gctgcgatcc gcgcgggtaa aaccattttg ctggccaata aagaatcact
    35941 ggttacctgc ggacgtctgt ttatggacgc cgtaaagcag agcaaagcgc aattgttacc
    36001 ggtcgatagc gaacataacg ctatttttca gagtttaccg caacctatcc agcataatct
    36061 gggatacgct gaccttgagc aaaatggcgt ggtgtccatt ttacttaccg ggtctggtgg
    36121 ccctttccgt gagacgccat tgcgcgattt ggcaacaatg acgcccgatc aagcttgccg
    36181 tcatccgaac tggtcgatgg gacgtaaaat ttctgtcgat tcggctacca tgatgaacaa
    36241 aggtctggaa tacattgaag cgcgttggct gtttaacgcc agcgccagcc agatggaagt
    36301 gctgattcac ccgcagtcag tgattcactc aatggtgcgc tatcaggacg gcagtgttct
    36361 ggcgcagctg ggggaaccgg atatgcgtac gccaattgcc cacaccatgg catggccgaa
    36421 tcgcgtgaac tctggcgtga agccgctcga tttttgcaaa ctaagtgcgt tgacatttgc
    36481 cgcaccggat tatgagcgtt atccatgcct gaaactggcg atggaggcgt tcgaacaagg
    36541 ccaggcagcg acgacagcat tgaatgccgc aaacgaaatc accgttgctg cttttcttgc
    36601 gcaacaaatc cgctttacgg atatcgctgc gttgaattta tccgtactgg aaaaaatgga
    36661 tatgcgcgaa ccacaatgtg tggacgatgt gttatctgtt gatgcgaacg cgagtgaagt
    36721 cgccagaaaa gaggtgatgc gtctcgcaag ctgaggataa tccggctaca gagagtcgcg
    36781 ctatttgtta gcgtagggct tcagtgatat agtctgcgcc atctgatcgt aagtagttgg
    36841 ctttataagg tcaggtatgc cgtggtttta cacggctttt ttttgtatag gcttcagtat
    36901 tcctgagtac cgtaaaccct gtcagggaat aaaaaacgcg tgatgttgtc tgctactcaa
    36961 ccacttagcg aaaaattgcc agcgcatggc tgccgtcatg ttgcgatcat tatggacggc
    37021 aatggtcgct gggcaaaaaa gcaagggaag attcgtgcct ttgggcataa agccggggca
    37081 aaatccgtcc gccgggctgt ctcttttgcg gccaacaacg gtattgaggc gttaacgctg
    37141 tatgccttta gtagtgaaaa ctggaaccga ccagcgcagg aagtcagtgc gttaatggaa
    37201 ctgtttgtgt gggcgctcga tagcgaagta aaaagtctgc accgacataa cgtgcgtctg
    37261 cgtattattg gcgataccag tcgttttaac tcgcgtttgc aagaacgtat tcgtaaatct
    37321 gaagcgctaa cagccggaaa taccggtctg acgctgaata ttgcggcgaa ctacggtgga
    37381 cgttgggata tagtccaggg agtcaggcaa ctggctgaaa aggtgcagca aggaaacctg
    37441 caaccagatc agatagatga agagatgtta aaccagcatg tctgtatgca tgaactggcc
    37501 cctgtagatt tagtaattag gactggggga gagcatcgca ttagtaactt tttgctttgg
    37561 caaattgcct atgccgaact ttactttaca gatgttctct ggcccgattt cgatgaacaa
    37621 gactttgaag gggcgttaaa tgcctttgct aatcgagagc gtcgtttcgg cggcaccgag
    37681 cccggtgatg aaacagcctg atgggggtcg cttttgctga agtatcgcct gatatctgct
    37741 tttgtgttaa tacccgtcgt catcgcggcg ttgtttctgt tgccgccggt ggggttcgcc
    37801 attgtaacgc tggtggtctg catgctggca gcgtgggaat ggggacagct tagcggtttt
    37861 accactcgtt cgcagcgagt atggttggcg gtgttatgcg ggttattgtt ggcgctgatg
    37921 ctttttctgt tgccggaata tcaccgaaat attcatcaac cgctggttga aatctcactt
    37981 tgggcttcgt tgggttggtg gattgtcgcg ctattgctgg tgctgtttta cccaggttcc
    38041 gcagcaatct ggcgtaactc taaaacattg cgccttattt ttggcgtgct aaccattgtt
    38101 cccttcttct ggggcatgct ggcgttacgg gcctggcact atgacgagaa tcattacagt
    38161 ggcgcaatat ggctgctcta tgtcatgatc ctggtatggg gcgctgactc cggcgcatat
    38221 atgtttggca aattgtttgg taaacataag ctggcaccga aggtttctcc gggtaaaacc
    38281 tggcaaggct ttataggtgg actcgctact gcagcggtaa tctcatgggg ttatggcatg
    38341 tgggcgaatc tcgacgtcgc tcccgtcacc ttactcattt gctctattgt cgcagcgtta
    38401 gcctcagtgc tcggcgatct gaccgagagt atgtttaagc gtgaagcagg aattaaggac
    38461 agcggtcatt taattccagg acacggtggt attttagatc gtattgatag cctgacggct
    38521 gcggtaccgg tctttgcttg cttgttgtta ctggtattca ggacgcttta acggaaggta
    38581 atatgctgag ttttctctgg gatttggctt cgttcatcgt tgcactgggt gtacttatca
    38641 ccgtgcatga atttggtcat ttctgggttg cccggcgttg tggtgttcgc gttgagcgtt
    38701 tctcaatagg gtttggtaag gcgctctggc ggcgaactga taagctcggc accgaatatg
    38761 ttatcgccct gatcccgttg ggcggttatg tcaaaatgct ggatgagcgc gcagaaccgg
    38821 tcgttccgga actccgccac catgccttca ataataaatc tgtcggccaa cgagcggcga
    38881 ttattgccgc aggtccggtt gcaaacttca tttttgctat ctttgcctac tggctggttt
    38941 ttattattgg tgtgcctggc gtacgtccgg tggttggcga aatagcagcc aattcgatag
    39001 ctgcggaagc acaaattgca ccaggtacgg aactaaaagc cgtagatggt atcgaaacgc
    39061 ctgattggga tgccgtgcgt ttgcagttgg tcgataaaat tggcgatgaa agcaccacca
    39121 ttacagtagc gccatttggc agcgaccaac ggcgggatgt aaagctcgat ttacgtcact
    39181 gggcgtttga gcctgataaa gaagatccgg tatcttcgct ggggattcgt cctcgtgggc
    39241 cgcaaattga acctgtactg gaaaatgtgc agccaaactc ggcggcaagc aaggcaggtt
    39301 tgcaagcagg cgacaggatc gttaaagtcg atggtcagcc cttaacgcag tgggtgacct
    39361 ttgtgatgct tgtccgggat aacccgggta aatccttagc gttagaaatc gaaaggcagg
    39421 ggagtccctt gtctttgaca ttaatcccgg ggagtaaacc gggtaatggt aaagcgattg
    39481 gttttgtcgg tattgagccg aaagtcattc ctttgccaga tgagtataaa gttgtacgcc
    39541 agtatgggcc gttcaacgcc atcgtcgaag ccacggacaa aacgtggcag ctgatgaagc
    39601 tgacggtcag tatgctggga aaattgatca ccggtgatgt gaaactgaac aacctcagtg
    39661 ggccgatctc tatcgccaag ggggctggga tgacagcgga actcggggta gtttattacc
    39721 tgccgtttct tgcgcttatt agcgtgaact tagggataat taacctgttt ccgttgcccg
    39781 tacttgacgg ggggcatctg ctgttccttg cgatcgaaaa gatcaagggc ggaccggtat
    39841 ccgagcgggt tcaagacttt tgttatcgca ttggctcgat tctgctggtg ctgttaatgg
    39901 ggcttgcact tttcaatgat ttctctcggt tatgagagtt agttaggaag aacgcataat
    39961 aacgatggcg atgaaaaagt tgctcatagc gtcgctgctg tttagcagcg ccaccgtata
    40021 cggtgctgaa gggttcgtag tgaaagatat tcatttcgaa ggccttcagc gtgtcgccgt
    40081 tggtgcggcc ctcctcagta tgccggtgcg tacaggcgac acggttaatg atgaagatat
    40141 cagtaatacc attcgcgctc tgtttgctac cggcaacttt gaggatgttc gcgtccttcg
    40201 tgatggtgat acccttctgg ttcaggtaaa agaacgtccg accattgcca gcattacttt
    40261 ctccggtaac aaatcggtga aagatgacat gctgaagcaa aacctcgagg cttctggtgt
    40321 gcgtgtgggc gaatccctcg atcgcaccac cattgccgat atcgagaaag gtctggaaga
    40381 cttctactac agcgtcggta aatatagcgc cagcgttaaa gctgtcgtga ccccgctgcc
    40441 gcgcaaccgt gttgacctaa aactggtgtt ccaggaaggt gtgtcagctg aaatccagca
    40501 aattaacatt gttggtaacc atgctttcac caccgacgaa ctgatctctc atttccaact
    40561 gcgtgacgaa gtgccgtggt ggaatgtggt aggcgatcgt aaataccaga aacagaaact
    40621 ggcgggcgat cttgaaaccc tgcgcagcta ctatctggac cgcggttatg cccgtttcaa
    40681 catcgactct acccaggtca gtctgacgcc agataaaaaa ggtatttacg tcacggtgaa
    40741 catcaccgaa ggcgatcagt acaagctttc tggcgttgaa gtgagcggca accttgccgg
    40801 gcactccgct gaaattgagc agctgactaa gatcgagccg ggcgagctgt ataacggcac
    40861 taaagtgacc aaaatggaag atgacattaa aaagcttctc ggtcgctatg gttatgccta
    40921 tccgcgcgta cagtcgatgc ctgaaattaa cgatgccgac aaaaccgtta aattacgcgt
    40981 gaacgttgat gtgggtaacc gtttctacgt gcgtaagatc cgttttgaag gtaacgatac
    41041 ctcgaaagat gccgtcctgc gtcgcgaaat gcgtcagatg gaaggtgcat ggttggggag
    41101 cgatctggtc gatcagggta aggagcgtct gaatcgtctg ggcttctttg aaactgtcga
    41161 taccgatacc caacgtgttc cgggtagccc ggaccaggtt gatgtagtct acaaggtgaa
    41221 agaacgtaac actggtagct tcaacttcgg gattggttac ggtactgaaa gtggcgtgag
    41281 cttccaggct ggcgtgcaac aggataactg gttaggtaca ggttatgctg ttggtatcaa
    41341 cgggaccaaa aacgattacc agacctatgc tgaactgtcg gtaaccaacc cgtacttcac
    41401 cgtagatggc gtaagcctcg gtggtcgtct cttctataat gacttccagg cagatgacgc
    41461 cgacctgtcc gactatacca acaagagtta tggtacagac gtgacgttgg gcttcccgat
    41521 taacgaatat aactcgctgc gtgcaggtct gggttatgta cataactccc tgtccaacat
    41581 gcagcctcag gttgcgatgt ggcgttatct gtactctatg ggtgaacatc cgagcacctc
    41641 tgatcaggat aacagcttca aaacggacga cttcacgttc aactatggtt ggacctataa
    41701 caagcttgac cgtggttact tcccgacaga tggttcacgt gtcaacctga ccggtaaagt
    41761 gaccattcct ggatcggata acgaatacta caaagtgacg ttagacacgg cgacttatgt
    41821 gccgatcgat gacgatcaca aatgggttgt tctgggtcgt acccgctggg gttatggtga
    41881 tggtttaggc ggcaaagaga tgccgttcta cgagaacttc tatgccggtg gttccagcac
    41941 cgtgcgtggc ttccagtcca ataccattgg tccgaaagca gtttacttcc cgcatcaggc
    42001 cagtaattat gatccggact atgattacga atgtgcgact caggacggcg cgaaagacct
    42061 gtgtaaatcg gatgatgctg taggcggtaa cgccatggcg gttgccagcc tcgagttcat
    42121 caccccgacg ccgtttatta gcgataagta tgctaactcg gttcgtactt cattcttctg
    42181 ggatatgggt accgtttggg atacaaactg ggattccagc caatattctg gatatccgga
    42241 ctatagtgat ccaagcaata tccgtatgtc tgcgggtatc gcattacaat ggatgtcccc
    42301 attggggccg ttggtgttct cctacgccca gccgttcaaa aagtacgatg gagacaaggc
    42361 agaacagttc cagtttaaca tcggtaaaac ctggtaagtg ttctccacaa aggaatgtag
    42421 tggtagtgta gcgatgactt taggcgatca atataagatc gccgggccac gcaaagaact
    42481 gcaccctccg gtgcaaatgg gatggtaagg agtttattgt gaaaaagtgg ttattagctg
    42541 caggtcttgg tttagcactg gcaacttctg ctcaggcggc tgacaaaatt gcaatcgtca
    42601 atatgggcag cctgttccag caggtagcgc agaaaaccgg tgtttctaac acgctggaaa
    42661 atgagttcaa aggccgtgcc agcgaactgc agcgtatgga aaccgatctg caggctaaaa
    42721 tgaaaaaact gcagtccatg aaagcgggca gcgatcgcac caaactggaa aaagacgtga
    42781 tggctcagcg ccagactttt gctcagaaag cgcaggcttt tgagcaggat cgcgcacgtc
    42841 gttccaacga agaacgcggc aaactggtta ctcgtatcca gactgctgtg aaatccgttg
    42901 ccaacagcca ggatatcgat ctggttgttg atgcaaacgc cgttgcttac aacagcagcg
    42961 atgtaaaaga catcactgcc gacgtactga aacaggttaa ataagtaatg ccttcaattc
    43021 gactggctga tttagcgcag cagttggatg cagaactaca cggtgatggc gatatcgtca
    43081 tcaccggcgt tgcgtccatg caatctgcac aaacaggtca cattacgttc atggttaacc
    43141 caaaataccg tgagcattta ggcttgtgcc aggcgtccgc ggttgtcatg acccaggacg
    43201 atcttccttt cgcgaaaagt gccgcgctgg tagtgaagaa tccctacctg acttacgcgc
    43261 gcatggcgca aattttagat accacgccgc agcccgcgca gaacattgca cccagtgcgg
    43321 tgatcgacgc gacggcgaag ctgggtaaca acgtatcgat tggcgctaac gcggtgattg
    43381 agtccggcgt tgaactgggc gataacgtga ttatcggtgc cggttgcttc gtaggtaaaa
    43441 acagcaaaat cggtgcaggt tcgcgtctct gggcgaacgt aaccatttac catgagatcc
    43501 agatcggtca gaattgcctg atccagtccg gaacagtggt aggcgcagac ggctttggtt
    43561 atgccaacga tcgtggtaac tgggtgaaga tcccacagat tggtcgcgta attattggcg
    43621 atcgcgtgga gatcggtgcc tgcacaacca tcgatcgcgg cgcgctggat gacactgtta
    43681 ttggcaatgg cgtgatcatt gataaccagt gccagattgc acataacgtc gtgattggcg
    43741 acaatacggc ggttgccggt ggcgtcatta tggcgggcag cctgaaaatt ggtcgttact
    43801 gcatgatcgg cggagccagc gtaatcaacg ggcatatgga aatatgcgac aaagtgacgg
    43861 ttacgggcat gggtatggtg atgcgtccca tcactgaacc aggcgtctat tcctcaggca
    43921 ttccgctgca acccaacaaa gtctggcgca aaaccgctgc actggtgatg aacattgatg
    43981 acatgagcaa gcgtctgaaa tcgcttgagc gcaaggttaa tcaacaagac taacgttcca
    44041 tcttttgttc gccaaacttt acggcctgtc tcattcttac gattgcggca ggccgtgtta
    44101 ttattgtcgt ttcttatatt ttgacaggaa gagtatcttg actactaaca ctcatactct
    44161 gcagattgaa gagattttag aacttctgcc gcaccgtttc ccgttcttac tggtggatcg
    44221 cgtgctggat tttgaagaag gtcgttttct gcgcgcagta aaaaatgtct ctgtcaatga
    44281 gccattcttc cagggccatt tccctggaaa accgattttc ccgggtgtgc tgattctgga
    44341 agcaatggca caggcaacag gtattctggc gtttaaaagc gtaggaaaac tggaaccggg
    44401 tgagctgtac tacttcgctg gtattgacga agcgcgcttc aagcgcccgg tcgtgcctgg
    44461 cgatcaaatg atcatggaag tcactttcga aaaaacgcgc cgcggcctga cccgttttaa
    44521 aggggttgct ctggtcgatg gtaaagtagt ttgcgaagca acgatgatgt gtgctcgtag
    44581 ccgggaggcc tgatacgtga ttgataaatc cgcctttgtg catccaaccg ccattgtgga
    44641 agagggcgcg tcaattggcg cgaacgcaca cattggtcct ttttgtatcg ttggacccca
    44701 tgtcgaaatt ggtgagggta ccgtactgaa atctcacgtt gtcgtgaatg gtcatactaa
    44761 aattggccgc gataatgaga tttatcagtt tgcctccatc ggcgaagtta accaggattt
    44821 gaaatatgct ggcgaaccga cccgtgtgga aatcggcgat cgtaaccgca ttcgcgaaag
    44881 cgtcaccatt catcgtggca cagtccaggg cggtggattg acgaaggtgg gcagcgacaa
    44941 cttactgatg atcaacgcgc acattgcgca cgattgtacg gtaggtaacc gctgtattct
    45001 cgccaacaac gcaacgctgg cgggtcacgt atcggttgac gacttcgcga tcatcggcgg
    45061 catgaccgca gtccatcagt tctgcatcat tggtgcgcat gtgatggttg gcggctgctc
    45121 cggtgtggcg caggacgtcc ctccttatgt cattgcgcag ggtaaccacg caacgccgtt
    45181 cggtgtcaat atcgaagggc tgaagcgccg cggattcagc cgtgaggcga ttaccgctat
    45241 ccgcaatgcg tataagctaa tttatcgtag cggtaaaacg ctcgatgaag tgaaaccgga
    45301 aattgctgaa ctggcggaaa catatccgga agtgaaagcc tttaccgatt tctttgcacg
    45361 ctcaacgcgc ggtctgattc gttaatgact gaccagcgtc cattaacgat tgccctggtc
    45421 gccggagaaa cctccggcga tatcctgggg gccggtttaa tccgcgctct gaaagaacgt
    45481 gtgcccaacg cccgctttgt tggtgttgcc gggccacgaa tgcaggctga aggctgcgaa
    45541 gcctggtacg aaatggaaga actggcggtg atgggcattg ttgaagtgct cggtcgtctg
    45601 cgtcgcttac tgcatattcg tgccgatctg acaaagcgtt ttggcgaact gaagccagat
    45661 gtttttgttg gtattgatgc gcccgacttc aatattactc ttgaaggtaa cctcaaaaag
    45721 cagggtatca aaaccattca ttacgtcagt ccatccgtct gggcgtggcg acagaaacgc
    45781 gttttcaaaa taggcagagc caccgatctg gtgctcgcat ttctgccttt cgaaaaagcg
    45841 ttttatgaca aatacaatgt accgtgccgc tttatcggtc ataccatggc tgatgccatg
    45901 ccgttagatc cagataaaaa tggtgcccgt gatgtgctgg ggatccctta cgatgcccac
    45961 tgtctggcat tgttgccggg cagccgtggt gcagaagtcg aaatgcttag tgccgatttc
    46021 ttgaaaactg cccagctttt gcgccagaca tatccggatc tcgaaatcgt ggtgccgctg
    46081 gtgaatgcca aacgccgcga gcagtttgaa cgcatcaaag ctgaagtcgc gccagacctg
    46141 tcggttcatt tgctggatgg aatgggccgt gaggcgatgg tcgccagcga tgcggcacta
    46201 ctggcgtcgg ggacggcagc cctggagtgt atgctggcga aatgcccgat ggtggtggga
    46261 tatcgcatga agccttttac cttctggttg gcgaagcggc tggtgaaaac tgattatgtc
    46321 tcgctgccaa atctgctggc gggcagagag ttagtcaaag agttattgca ggaagagtgt
    46381 gagccgcaaa aactggctgc ggcgctgtta ccgctgttgg cgaacgggaa aaccagccac
    46441 gcgatgcacg ataccttccg tgaactgcat cagcagatcc gctgcaatgc cgatgagcag
    46501 gcggcacaag ccgttctgga gttagcacaa tgatcgaatt tgtttatccg cacacgcagc
    46561 tggttgcggg tgtggatgaa gtcggacgcg ggccgttagt tggcgcggtc gtcaccgctg
    46621 cggtgatcct tgacccggcg cgcccgattg ccgggctgaa tgattccaaa aagctgagcg
    46681 aaaaacgccg tctggcgctc tatgaagaga tcaaagagaa agcgttgagc tggagtctgg
    46741 gccgcgcgga accccacgaa atcgacgagc tgaacattct tcatgcgacc atgctggcga
    46801 tgcagcgtgc cgtcgctggg ctgcatattg cgccggaata tgtgttgatt gatggtaacc
    46861 gctgcccgaa attaccgatg cctgcgatgg ctgtggtgaa aggggatagc cgcgtaccag
    46921 aaatcagtgc cgcgtctatc ctggcgaaag tgacgcgtga cgccgaaatg gcggcgctgg
    46981 atattgtttt cccgcaatat ggttttgccc aacataaagg gtatccaacc gcttttcatc
    47041 tggaaaaact ggctgaatat ggcgcgactg aacatcatcg tcgcagcttt gggcctgtca
    47101 aacgcgcact gggacttgcg tcctgattct tgtgtcgaga ttaagtaaac cggaatctga
    47161 agatgtctga accacgtttc gtacacctgc gggtgcacag cgactactcg atgatcgatg
    47221 gcttggccaa aaccgcaccg ctggtaaaaa aggcggcggc gttgggtatg ccagcactgg
    47281 cgatcaccga tttcaccaac ctttgcggtc tggtgaagtt ctacggagcg ggacatggcg
    47341 cagggattaa gcctatcgtc ggggcagatt ttaatgtcca gtgcgacctg ctgggtgatg
    47401 agttaaccca cctgacggta ctggcggcga acaataccgg ctatcagaat ctggcgttgc
    47461 tgatctcaaa agcgtatcag cgcgggtacg gtgccgccgg gccgatcatc gatcgcgact
    47521 ggcttatcga attaaacgaa gggttgatcc ttctttccgg cgggcgcatg ggcgacgtcg
    47581 gacgcagtct tttgcgtggt aacagcgcgc tggtagatga gtgtgtcgcg ttttatgaag
    47641 aacacttccc ggatcgctat tttctcgagc tgatccgcac cggcaggccg gatgaagaaa
    47701 gctatctgca cgcggcggtg gaactggcgg aagcgcgcgg tttgcccgtc gtggcgacta
    47761 acgatgtgcg ctttatcgac agcagcgact ttgacgcaca cgaaatccgc gtcgcgatcc
    47821 acgacggctt taccctcgac gatcctaaac gcccgcgtaa ctattcgccg cagcaatata
    47881 tgcgtagcga agaggagatg tgtgagctgt ttgccgacat cccagaagcc cttgccaaca
    47941 ccgttgagat cgccaaacgc tgtaacgtaa ctatgcgtct tggtgaatac ttcctgccgc
    48001 agttcccgac cggggacatg agcaccgaag attatctggt caagcgtgca aaagaaggcc
    48061 tggaagagcg tctggcattt ttattccctg acgaggaaga acgtcttaaa cgccgcccgg
    48121 aatatgacga acgtctggag actgaacttc aggttatcaa ccagatgggc ttcccgggct
    48181 acttcctcat cgttatggaa tttatccagt ggtcgaaaga taacggcgta ccggtagggc
    48241 caggccgtgg ctccggtgcg ggttcactgg tggcctacgc gctgaaaatc accgacctcg
    48301 atccgctgga atttgacctg ctgttcgaac gtttccttaa cccggaacgt gtctccatgc
    48361 ctgacttcga cgttgacttc tgtatggaga aacgcgatca ggttatcgaa cacgtggcgg
    48421 acatgtacgg tcgtgatgcg gtatcgcaga ttatcacctt cggtacgatg gcggcgaaag
    48481 cggtgatccg cgacgtaggc cgcgtgctgg ggcatccgta cggctttgtc gaccgtatct
    48541 cgaaactgat cccgcccgat ccggggatga cgctggcgaa agcgtttgaa gccgagccgc
    48601 agctgccgga aatctacgaa gcggatgaag aagttaaggc gctgatcgac atggcgcgca
    48661 aactggaagg ggtcacccgt aacgccggta agcacgccgg tggggtggtt atcgcgccga
    48721 ccaaaattac cgattttgcg ccgctttatt gcgatgaaga gggcaaacat ccggtcaccc
    48781 agtttgataa aagcgacgtt gaatacgccg gactggtgaa gttcgacttc cttggtttgc
    48841 gtacgctcac catcatcaac tgggcgctgg agatgatcaa caagcggcgg gcgaagaatg
    48901 gcgagccacc gctggatatc gccgcgatcc cgctggatga caagaaaagc ttcgacatgc
    48961 tgcaacgctc ggaaaccacg gcggtattcc agcttgaatc gcgcggcatg aaggacctga
    49021 tcaagcgtct gcaacctgac tgcttcgaag atatgatcgc actggtggca ctgttccgcc
    49081 ctggtccgtt gcagtcaggg atggtggata actttatcga ccgtaaacat ggtcgtgaag
    49141 agatctccta tccggacgtg cagtggcagc atgaaagcct gaaatcggta ctggagccaa
    49201 cctacggcat tatcttgtat caggaacagg tcatgcagat tgcccaggtg ctttctggtt
    49261 ataccctcgg tggcgcggat atgctgcgcc gtgcgatggg taagaaaaag ccggaagaga
    49321 tggctaagca gcgttctgta tttgctgaag gtgcagaaaa gaacggaatc aacgccgaac
    49381 tggcgatgaa aatcttcgac ctggtggaga aattcgcggg ttacggattt aacaaatcgc
    49441 actctgcggc ctatgctttg gtgtcatatc aaacgttatg gctgaaagcg cactatccgg
    49501 cggagtttat ggcggcggta atgaccgccg atatggacaa caccgagaag gtggtgggcc
    49561 tggtggatga gtgctggcgg atggggctga aaatcctgcc accagatata aactccggtc
    49621 tttaccattt ccacgtcaac gacgacggcg aaatcgtgta tggtattggc gccatcaaag
    49681 gggtaggtga aggtccgatt gaggccatca tcgaagcccg taataaaggc ggctacttcc
    49741 gcgaactgtt tgatctctgc gcccgtaccg acaccaaaaa gttaaaccgg cgggtgctgg
    49801 aaaaactgat catgtccggg gcgtttgacc gtcttgggcc acaccgcgcg gcgctgatga
    49861 actcgctggg tgatgcgtta aaagcggcag atcaacacgc gaaagcggaa gctatcggtc
    49921 aggccgatat gttcggcgtg ctggccgaag agccggaaca aattgaacaa tcctacgcca
    49981 gctgccaacc gtggccggag caggtggtat tagatgggga acgtgaaacg ttaggcctgt
    50041 acctgaccgg acaccctatc aaccagtatt taaaagagat tgagcgttat gtcggaggcg
    50101 taaggctgaa agacatgcac ccgacagaac gtggtaaagt catcacggct gcggggctcg
    50161 ttgttgccgc gcgggttatg gtcaccaagc gcggcaatcg tatcggtatc tgcacgctgg
    50221 atgaccgttc cgggcggctg gaagtgatgt tgtttactga cgccctggat aaataccagc
    50281 aattgctgga aaaagaccgc atacttatcg tcagcggaca ggtcagcttt gatgacttca
    50341 gcggtgggct taaaatgacc gctcgcgaag tgatggatat tgacgaagcc cgggaaaaat
    50401 atgctcgcgg gcttgctatc tcgctgacgg acaggcaaat tgatgaccag cttttaaacc
    50461 gactccgtca gtctctggaa ccccaccgct ctgggacaat tccagtacat ctctactatc
    50521 agagggcgga tgcacgcgcg cggttgcgtt ttggcgcgac gtggcgtgtc tctccgagcg
    50581 atcgtttatt aaacgatctc cgtggcctca ttggttcgga gcaggtggaa ctggagtttg
    50641 actaatacag gaatactatg agtctgaatt tccttgattt tgaacagccg attgcagagc
    50701 tggaagcgaa aatcgattct ctgactgcgg ttagccgtca ggatgagaaa ctggatatta
    50761 acatcgatga agaagtgcat cgtctgcgtg aaaaaagcgt agaactgacg cgtaaaatct
    50821 tcgccgatct cggtgcatgg cagattgcgc aactggcacg ccatccgcag cgtccttata
    50881 ccctggatta cgttcgcctg gcatttgatg aatttgacga actggctggc gaccgtgcgt
    50941 atgcagacga taaagctatc gtcggtggta tcgcccgtct cgatggtcgt ccggtgatga
    51001 tcattggtca tcaaaaaggt cgtgaaacca aagaaaaaat tcgccgtaac tttggtatgc
    51061 cagcgccaga aggttaccgc aaagcactgc gtctgatgca aatggctgaa cgctttaaga
    51121 tgcctatcat cacctttatc gacaccccgg gggcttatcc tggcgtgggc gcagaagagc
    51181 gtggtcagtc tgaagccatt gcacgcaacc tgcgtgaaat gtctcgcctt ggcgtaccgg
    51241 tagtttgtac ggttatcggt gaaggtggtt ctggcggtgc gctggcgatt ggcgtgggcg
    51301 ataaagtgaa tatgctgcaa tacagcacct attccgttat ctcgccggaa ggttgtgcgt
    51361 ccattctgtg gaagagcgcc gacaaagcgc cgctggcggc tgaagcgatg ggtatcatcg
    51421 ctccgcgtct gaaagagctg aaactgatcg actccatcat cccggaaccg ctgggcggcg
    51481 ctcaccgtaa cccggaagcg atggcggcat cgttgaaagc gcaactgctg gcggatctgg
    51541 ccgatctcga tgtgttaagc actgaagatt taaaaaatcg tcgttatcag cgcctgatga
    51601 gctacggtta cgcgtaattc gcaaaagttc tgaaaaaggg tcacttcggt ggcccttttt
    51661 tatcgccacg gtttgagcag gctatgatta aggaaggatt ttccaggagg aacacatgaa
    51721 catcattgcc attatgggac cgcatggcgt cttttataaa gatgagccca tcaaagaact
    51781 ggagtcggcg ctggtggcgc aaggctttca gattatctgg ccacaaaaca gcgttgattt
    51841 gctgaaattt atcgaacata accctcgaat ttgcggcgtg atttttgact gggatgagta
    51901 cagtctcgat ttatgcagcg atatcaatca acttaatgaa tatctcccgc tttatgcctt
    51961 catcaacacc cactcgacga tggatgtcag cgtgcaggat atgcggatgg cgctctggtt
    52021 ttttgaatat gcgctggggc aggcggaaga tatcgccatt cgtatgcgtc agtacaccaa
    52081 cgaatatctt gataacatta cgccgccgtt cacgaaagcc ttgtttacct acgtcaaaga
    52141 gcggaagtac accttttgta cgccggggca tatgggcggc accgcatatc aaaaaagccc
    52201 ggttggctgt ctgttttatg attttttcgg cgggaatacc cttaaggctg atgtctctat
    52261 ttcggtcacc gagcttggtt cgttgcttga ccacaccggg ccacatctgg aagcggaaga
    52321 gtacatcgcg cggacttttg gcgcggaaca gagttatatc gttaccaacg gaacatcgac
    52381 gtcgaacaaa attgtgggta tgtacgccgc gccatccggc agtacgctgt tgatcgaccg
    52441 caactgccat aaatcactgg cgcatctgtt gatgatgaac gatgtagtgc cagtctggct
    52501 gaaaccgacg cgtaatgcgt tggggattct gggggggatc ccgcgccgtg aatttactcg
    52561 cgacagcatc gaagagaaag tcgctgccac tacgcaagca caatggccgg ttcatgcggt
    52621 gatcaccaac tccacctatg atggtttgct ctacaacacc gactggatca aacagacgct
    52681 ggatgtcccg tcgatccact tcgattccgc ctgggtgccg tatactcatt ttcatccaat
    52741 ctaccagggt aaaagtggta tgagcggcga gcgtgttgcg ggaaaagttt tcttcgaaac
    52801 gcaatcgacc cacaaaatgc tggcggcgtt atcgcaggct tcactgatcc acattaaagg
    52861 cgagtatgac gaagaggcgt ttaacgaagc ctttatgatg cataccacca cctcgcccag
    52921 ttatcccatt gttgcttcgg ttgagacggc ggcggcgatg ctgcgtggta atccgggcaa
    52981 acggctgatt aaccgttcgg tggagcgggc gctgcatttt cgcaaagagg tccagcggct
    53041 gcgggaagag tctgacggtt ggtttttcga tatctggcaa ccgccgcagg tggatgaagc
    53101 cgaatgcagg cccgttgcgc ctggcgaaca atggcacggc tttaacgatg cggatgccga
    53161 tcatatgttt ctcgatccgg ttaaagtcac tattttgaca ccggggatgg acgaacaggg
    53221 caatatgagt gaggagggga tcccggcggc gctggtagca aaattcctcg acgaacgtgg
    53281 gatcgtagta gagaaaaccg gcccttataa cctgctgttt ctctttagta ttggcatcga
    53341 taaaaccaaa gcaatgggat tattgcgtgg gttgacggaa tttaagcgct cttacgatct
    53401 caacctgcgg atcaaaaata tgctgcccga tctctatgct gaagatcccg atttctacca
    53461 caatatgcgt attcaggatc tggcgcaagg gatccataag ctgattcgta aacacgatct
    53521 ttccggtttg atgttgcggg cattcgatac tttgccggaa atgatcatga cgccacatca
    53581 ggcatggcag agacagatta aaggtgaagt agaaaccatt gcgctggaac aactggtcgg
    53641 tagagtatcg gcaaatatga tcctgcctta tccaccgggc gtaccgctgt tgatgccagg
    53701 agaaatgctg accaaagaga gccgcacggt actcgatttt ctactgatgc tttgttccgt
    53761 agggcaacat taccccggtt ttgaaacgga tattcacggc gcgaaacagg acgaagacgg
    53821 cgtttaccgc gtacgagtcc taaaaatggc gggataactt gccagagcgg cttccgggcg
    53881 agtaacgtgc tgttaacaaa taaaggagac gttatgctgg gtttaaaaca ggttcaccat
    53941 attgcgatta ttgcgacgga ttatgcggtg agcaaagctt tctactgcga tattcttggt
    54001 ttcacgctgc aaagcgaagt ctatcgcgaa gcgcgcgact cgtggaaagg ggatttggcg
    54061 cttaatgggc aatatgtgat tgagcttttc tcttttccgt tcccgccgga gcgccccagc
    54121 cgaccggaag cttgcggcct gcgtcatctg gcttttagcg ttgatgacat cgatgcggca
    54181 gtggcgcacc ttgaaagcca taacgtgaag tgtgaagcca tccgtgtcga tccatacacg
    54241 caaaaacgct tcaccttctt taacgatccg gacgggctgc cgttggaact gtatgagcag
    54301 taaggcttgt catcgccgca tttgcccggt aacgtgccgg gcattgctac tgtaaaatcg
    54361 caccatcatg acactcacgc tcaatagaca acttctcacc tcacgccaga ttctggtggc
    54421 ctttagcggc gggcttgact ccaccgttct gctgcatcag ttggtgcagt ggcggacgga
    54481 aaatccgggc gtcactctgc gcgctattca tgtgcatcac ggtttaagtg ccaatgccga
    54541 tgcctgggtt acgcattgcg aaaacgtctg ccaacagtgg caggtgccgc tggtggtcga
    54601 acgcgtacaa cttgcgcaag aggggctggg gattgaggcc caggcgcggc aggcacgtta
    54661 tcaggcattt gcccgtaccc tattgcccgg cgaagtgctg gtcaccgcgc aacatctcga
    54721 cgatcaatgt gaaacctttc tgctggcgct aaaacgcggt agcggccctg ccgggctttc
    54781 ggctatggcg gaagtttcgg agtttgctgg aacacggctt atccgcccgt tgcttgcccg
    54841 cacgcggggg gaactggagc agtgggcatt ggcgcatggt ttacgctgga ttgaagacga
    54901 aagtaatcag gacgactcat acgatcgtaa ctttctgcgc ctgcgcgtag tgccgttatt
    54961 gcagcagcgt tggccgcatt ttgcggaagc aacggcccgc agcgccacac tttgtgctga
    55021 acaagagagt ctgttggatg aactgctggc ggatgattta gcacactgcc aaacgtcgca
    55081 ggggacgctg cagattgcgc caatgctggc gatgagtgat gcccgtcgcg cggcgattat
    55141 tcgccgctgg ctggcagggc agaatgcacc gatgccttcc cgcgacgcgc tggtgcgcat
    55201 ctggcaggaa gtggcgctgg cgcgggaaga tgcttccccc tgtttacgtt tgggcgcctt
    55261 tgaaatccgc cgctatcagt cgcaactgtg gtggattaaa tccgtcaccg ggcaaagcga
    55321 aaccattgtg ctgtggcaga cgtggctaca accgctggaa ttaccggcgg ggctgggaac
    55381 cgtacagctt actgcgggag gcgatattcg ccctccgcgt gcagacgaag cggtcagcgt
    55441 gcgtttcaaa gcgccaggat tgctgcatat tgtcgggcgc aacggcggac gtaagctgaa
    55501 gaaaatctgg caagagttag gcgtgccgcc gtggctacgt gacaccacgc cactgctgtt
    55561 ttatggagaa acgctgattg cggcggcagg ggtatttgtg acgcaagaag gtgtggctga
    55621 aggtgagaat ggcgtaagtt ttgtctggca gaaaacgctt agttaagtga aagccggata
    55681 agacgcatca aacgtcgcat ccggcgaaag tcaatcagga ttcgcttacc accaccgtac
    55741 cgatttcagg gtggctaaag ctggtaattt tatccagacg cagctcgcgg gtttcgccag
    55801 cggcctcgac gaccagatac tccacatttt tgcgggagac taaatcactg gcttttgcct
    55861 gcaatttttc gccatctttc agctcaagtg tcagtattaa atgatgctgg caggcgagct
    55921 caagattatc gtaatcatca caattaattg gttgatacgt atcattcatt gacataatcg
    55981 ctcaccagta agtttgccgc agcgtatgct gctttttccc tgacagcctc agaaagggcg
    56041 tcgtcggcag ccatttcatt cagcactttc aaaacgcagc ccagcgcgtc cggaacgtat
    56101 cctaagtctc cgctggcgat ttccgcgtac cgcttgcgta ttaactcaca atatttttcc
    56161 acatgccctc ctgtcagcac tctgacttaa ccgtggatgc aagtctaagc ctacgaagat
    56221 aaactctgtt tcgcaaggtg actataccac actcatttct gcaatatcag cgccgcaact
    56281 gcacgtattc cgttacaatg gcctcctgat tcgaaaggag ttttcttatg gcgcttaaag
    56341 cgacaattta taaagcgacg gttaatgtgg ccgatctcga ccgcaaccag tttctcgatg
    56401 cctctctgac gctggcgcgc cacccttcag aaacccagga gcgtatgatg ctgcgcttgc
    56461 tggcgtggct gaaatatgct gatgaacgtc tgcaatttac ccgtggtttg tgtgccgatg
    56521 atgagccgga agcgtggctg cgtaacgatc acctgggcat tgatttgtgg attgagctgg
    56581 ggctgccgga tgagcgacga attaagaaag cctgcaccca ggccgcagaa gtggcgctgt
    56641 ttacctataa tagtcgggcg gcgcaaatct ggtggcagca aaatcagagc aaatgtgtgc
    56701 agtttgccaa tctttccgtc tggtatctgg acgatgaaca actggcgaaa gtaagcgcct
    56761 ttgccgatcg taccatgacg ctgcaggcaa cgattcagga tggtgttatc tggttatcgg
    56821 atgataagaa taatctggaa gttaacttaa ccgcctggca acaaccttca tgattgtgat
    56881 ttcccgacat gttgctattc ccgatggtga gcttgagatc accgccattc gtgcgcaggg
    56941 cgctggcggg cagcatgtta ataagacctc aacggctatt catctgcgtt ttgacattcg
    57001 ggcgtccagc ctgccagagt attacaaaga gcgtctgctc gccgccagcc atcatttgat
    57061 cagcagtgat ggcgtgattg tcattaaggc acaggaatac cgcagtcagg aactgaaccg
    57121 cgaagccgct ctggcccggc tggtggcagt gattaaagat ttaacaaaag aacaaaaagc
    57181 ccgacgaccc acgcggccca cccgtgcatc gaaagagcgc aggctggcat cgaaagcaca
    57241 aaaatcaagc gtgaaggcga tgcgcggcaa agtacgcagc ggtcgggaat aaaaagaagg
    57301 aatggatggt gaaaaaagcg atagtgacag cgatggctgt aatcagcctc tttactctga
    57361 tgggatgtaa taatcgggcc gaagtcgata cgctttctcc ggcgcaggct gccgaactga
    57421 aaccgatgcc gcaaagttgg cgcggcgtgc taccgtgtgc cgattgcgaa ggaatcgaaa
    57481 tctctctgtt cctcgaaaaa gacggaacat gggtgatgaa tgagcgttat ctcggtgctc
    57541 gtgaagaacc ttcctccttc gcttcctacg gtacatgggc gcgaaccgct gacaagctgg
    57601 tattaaccga cagcaaaggt gaaaagtcat attatcgggc gaaaggcgat gcgctggaga
    57661 tgctcgatcg tgaaggcaat ccaattgaat cgcagttcaa ctatacgctg gaaccggcac
    57721 aatccagttt acctatgacg ccgatgaccc tgcgaggcat gtatttttat atggctgatg
    57781 cggcgacctt cactgattgc gcgaccggaa aacgtttcat ggtagcgaat aacgcagagc
    57841 tggagcgtag ctacctggct gcgcgcggtc acagtgaaaa accggtgtta ctgtcagtag
    57901 aaggtcactt tacgcttgag gctaatccgg atactggtgc gccaaccaaa gtattagcgc
    57961 ccgatacggc gggtaaattt tacccgaata aggattgcag tagtttgggg cagtaaacct
    58021 gcttaaggca gaaacaaacg caaaattgcc tgatgcgctg cgcttatcag gcctacagca
    58081 tataatgcaa cattttgaat tagcacgatt ttgtaggccg gataaggcgt ttacgccgca
    58141 tccggcattt tgtgcacgag gctggcttta tcactgagtc agcccaacaa agcgacccgc
    58201 cttgatataa atcccaggct tcaggtgccc aacatactgt aactgcgttt ctggcttaaa
    58261 cgcagagaca tcaccaatac gcatatgcat cagatcggca gggctaatcc agcccgattg
    58321 cgccagtgta agtggatgcc cggatttggc aaatgcatca gtgacaaatt ccgaacaaaa
    58381 ccacgacttt ttgtctcctt cgcacacact gcttaactgc gctttcgcca ggtcgctgac
    58441 gcactgttgg cgaaaatcct cggagaacgg attcagtgag cacatctggc gagtcaccat
    58501 aaagggaata aattcgacaa tgccgcgata gttataaccg ctatctttga ttttattggc
    58561 aaacgcggtg atatctgtgg cttgttgcgg ggtaagatcc gggactcgta agacgaaaag
    58621 cttatcacta tgcttcatcg cttttttaag ggaaacaatc tggacgccag cgcctgtcgc
    58681 ttctgcaacg ttattatcac ccagaaagat tgcaacgtga ctcacagagg aagtgctgaa
    58741 gacgcggatt ccaaatgagg ttaccccaag gcttgaggag aacagcaaat cgccgggttt
    58801 gagatcaggt gccgttattt cttttattga ttgttcggtg aaagagcttt gatgctggaa
    58861 tttgacagcc catgtttttg cctcggcatc tactgctgtg gctgatgggt ctggctggct
    58921 gatatcaacg gtacaagccg aaagtaaaag aaagctgggg agaagcaggc ggcagtacgc
    58981 ctttggttta tccattttat acaatccatg taaaaaaggg ccctgaaatt caggaccctt
    59041 tctggcatca gcctttaatc tgtttcacca gatattcgac gatgtcacca gtcttaatta
    59101 actgtttctc gccgttgcga cgatatttat attcgatatc gtcgttgtcg aggttacggt
    59161 cgcccagcac aatagtgtgc ggaataccga tcagttccat atcagcaaac atcacgcccg
    59221 gacgctcttt gcggtcatcc agcagcactt cgataccttg tgcacgcagt tcgctgtaca
    59281 gtttctcagc aagctcttgt acgcggaagg atttgtgcat gttcatcggc agaatcgcca
    59341 cctggaacgg cgcgatagcg tcaggccata cgatgccgcg ttcgtcgtag ttctgctcaa
    59401 tcgccgcagc taccacacgc gttaccccga taccgtagca acccatcgtc aggatttggt
    59461 tacggccatc ttcaccctgt acggaggctt tcagtgcttc ggagtacttg gtacccagct
    59521 ggaagatgtg accaacttcg ataccacgtt tgatcagcag cgtaccctgg ccatccgggc
    59581 ttggatcgcc agccaccacg ttacggatat ctgcaacttc cggggtagcg acatcgcgat
    59641 cccagttgat accgaagtag tgtttaccat cgatgttagc accagcagcg aaatcactca
    59701 tcgccgcaac ggtacggtca atcaccaccg gaatcggcat gtttaccgga cccagtgaac
    59761 ccggaccggc tttaaccaca gcacgaattt cttcttcggt cgcgaaagtc agcgggcttg
    59821 caacctgcgg cagtttttct gctttaactt cgttcagctc gtgatcaccg cgcaccagca
    59881 gcgcaaccag cgggaagcta ctgccttcaa ccgctttaac cagcagagtc ttaaccgttt
    59941 tctcaatcgg cagattgaac tgttcaacca gttccgcgat ggttttcgcg ttcggcgtat
    60001 caaccagcgt catttcctgg gtagcagcag cgcgcggttc tttcggcgcg atagcttctg
    60061 ccagttcaat gttcgctgca tagtcagagg tgtcggagaa gaccacatcg tcttcaccgc
    60121 tctgcgccag cacctggaat tcgtgagagg cgctgccgcc gatagaaccg gtgtcggcct
    60181 gtacggcgcg gaaatccagc cccatgcggc tgaagatttt gctgtaggcc gcatacattg
    60241 catcgtaggt ttcctgcagg gattcctgag aagtatggaa agagtaagca tctttcatca
    60301 ggaattcgcg ggaacgcatg acgccgaaac gcggacgcac ttcgtcgcgg aacttggtct
    60361 ggatctgata gaagttcagc ggcagctgtt tgtaagagct aagctcgtta cgaatcaggt
    60421 cagtgataac ttcttcatga gttgggccga gtacgaacgg acgctcgcca cggtcaacaa
    60481 aacgcagcag ttccggaccg tactgttccc aacgaccact ctcttgccac aaatcggctg
    60541 gctgaaccac cggcatcgac acctcgatcg caccggcgtt gttcatctct tcacgcacga
    60601 tgttttcgac ttttttcaga acgcgcacgc cggtcggcag ccaggtatat aacccggagg
    60661 ccagcttgcg gatcatcccg gcgcgcagca tcagctgatg gctgatcacc tcggcgtcgg
    60721 caggtgtctc cttgagagtg gagagcaggt attggctagt acgcatgttg ttacggttcc
    60781 agttggaagg tagaacaggc tcaaggcgag cctgggacaa aaaaagtgat ttagtttacc
    60841 agtgcaaaag aaatgtcaaa agagaagggc gtgaatttaa cgcggttcca gcgcaaagac
    60901 ttcaaaacct gcgtcggtga cgcgccagcg aacgttaaaa tcatgtagcc agacggcata
    60961 ggttttgccc gtttcctcac ctttacgata ggccgggcgc gggtcctgcg ccagtacttc
    61021 gcggataaac agcgttaact gcggataacg cttctccagc gtcaaaagct gcttttcgac
    61081 ctctgcggta aaactcaccg ccatctctgc agctggcgca ctttgcgcat agctggcact
    61141 ggcatcggga agcgattcgg caaaggggag atacggtttg atatccacta ccggcgtacc
    61201 atcgaccaga tccagactgc cgagcttcag aatcacgctg cctttatggc aaacaacctc
    61261 tttcagctct accagcgaca tgccaattgg gttagggcgg aaagtagagc gtgttgcgaa
    61321 aacccccatt ctggcgttac cgccgaggcg cggtggacgc acagtcggac gccagccgcc
    61381 ttccatcgtt tgatgaaaga cgaaaaggat ccataaatgg ctgaatgctt ccaggccgcg
    61441 aacggcgtcg gcctggttgt aggaagcaat gagatgcagt tcgccgttgg cgctttttac
    61501 cagacctggc tggcgcggaa cggcgaattt ttctttatag ggcgagcgaa taacgcctat
    61561 ttgctcgaac tggaaactgc tcatttcgcc gtaatgttaa gcgcagagcc gatacataca
    61621 gcctgacgat agcagcccgg cgtaccgctg gtgacttcgc agctatgcag taatacagca
    61681 ttggctttca ttttagaggc attgatttgc atctgcttac gtgcggttgg aatgctcggc
    61741 ggagagtcct gattagaggc ctggcaagag tcgccactga cttcaccgag atcgcggaac
    61801 ggtttgccga ctaattcttc tgcattggta taaattcgga ccggcgtggc gcgcggcgct
    61861 ttcggttttg caggctccgc tttcggctgg ggtgcagtgc tttgaacggg ttcgacaggg
    61921 gatctgctta acatggaaca gccgcttagc atgagtgcta ctaaacagat cggtaaagca
    61981 cgcatagtat ttcctcaatg tatgatcaaa acgtcaatat tgaatcagga gcttgtaaaa
    62041 atgacaagac gggcaagcgc ccgtcctgaa tgatattaca aattgtggaa acagcctaaa
    62101 aattaccagc ctttaacagc gccgccgtta aacactttgt ttgctgcttc gtaaacttca
    62161 tcagactgat aagcctggac gaatttcttc acgttctcgg cgtctttgtt atcttcacgc
    62221 gttacgatca ggtttacgta tggggagtct ttatcttcga caaagatacc gtctttcgcc
    62281 ggagtcaggc caatctggct ggcataggtg gtattgataa ctgccagagc gatttgcgcg
    62341 tcgtccagag aacgcggcag ttgcggtgct tccagttcaa caattttcag attttttggg
    62401 ttctcaacaa catcaagaac ggtcggcagc aggccaacgc catctttcag tttgatcaag
    62461 cccacttttt gcagcagcag cagtgaacga ccaagattag ttggatcgtt tggcacggca
    62521 acctgcgaac catcctgcag ttcatccagt gatttgattt tcttggagta accagcaatc
    62581 ggataaacaa aagtgttgcc tactgcgacc agtttgtagc cacgatcttt cagttgctga
    62641 tcaaggtacg gtttatgctg gaaggcgttg gcgtcgatat cgcctttgct caatgcttcg
    62701 tttggcagaa catagtcgtt gaaggttacc agctcaacgt ccaggccata tttgtctttc
    62761 gcaactttct gcgcgacttc tgctacctgc tgttcggcac caacaatcac gccgacttta
    62821 atgtggtttg gatctttttc atcctgaccg cagcctacca gtgccagtga tccaatcagg
    62881 gctcccactg ccgcaaaggt tttgaatttg aacgccatac cttattcctt cttcaattat
    62941 ttatgttgtg ttgaacgtta cttgcgagtg acagcccgga cgatgcggtc gcctgcgaac
    63001 tgaattaaat aaaccagaat gaccagcaat accagtaccg tattcatcac cgtcgcgttg
    63061 tagccgatgt agccatactg atagccaatc tgacctaaac caccggcacc gactgcacca
    63121 cccatcgcgg aataaccgac cagggtaatc agggtgatag ttgccgcatt caccagaccc
    63181 ggcaacgctt ccggtaacag cactttacag acgatctgca ttggcgtggc acccattgcg
    63241 cgggaagctt caattaaccc ggttgggatc tccagcagag cgttctcgac catacgggca
    63301 ataaacggtg ctgcaccaac ggttaacgga acaattgctg cctgcaaacc aatcgatgta
    63361 ccgacaataa cgcgggtaaa cggaatcatc cataccagca agataatgaa cgggatggaa
    63421 cggaaaatgt tcacaatcgc agaaacggta cgatacagct tcgcgttagc aataatttgc
    63481 cccggacgcg tgacataaag cagaacgcca accggcagac caatcacaaa cccaaaaaat
    63541 ccggatacga aggtcattgc cagcgtttcc catacgccac gaaccagcag ccacatcatc
    63601 ggctcagaca taacccagta cctctacttt tacatggtgt tcctgcagcc aggcaatggc
    63661 ggcttgcgta tcttgttgtg tgccgtgcat ttcagtcagc atgatgccga atttaacgcc
    63721 accggcgtag tccatctgcg cgctaataat gttgttgttg acgttgaaac gacgcgcggt
    63781 ttcagaaagc agtggggcat cgaccgattg accggtaaac tccagacgca gcatcggcac
    63841 gcagtcagta aatggctccg tttgcagacg ttcctggtaa tcttccggga tatccagatg
    63901 cagggtcgac tgaataaact tctgcgccag cggcgttttc ggatgcgaga acacttcact
    63961 taccgtgtcc tgctcgatca gttcgccatt gctgatgacc gccacgcaat cacaaatacg
    64021 cttcacaacg tccatttcgt gggtgatcaa cagaatcgtc aaacccagac ggcggttaat
    64081 gtctttcagc agttcgagaa tagaacgtgt cgttgccggg tccagcgcgc tggtggcttc
    64141 atcacacagc aatactttgg gattgctggc taatgcacgg gcaatcgcca cacgttgttt
    64201 ctgcccaccg gaaagattcg acgggtagct atcatgctta tcgccaagac caaccaatga
    64261 cagcaattcc gtcacgcgac gtttgatctc gtctttcggt gtgttgtcca gctccagcgg
    64321 cagagccacg ttgccaaaaa cagtacgcga agagagcagg ttaaaatgct ggaaaatcat
    64381 accaatctgg cggcgagctt tggtcaactc ggattctgac agcgtggtca attcctggcc
    64441 atcgaccagc acgctaccct cggttgggcg ctccagcagg tttacacaac gtataagcgt
    64501 actcttaccc gcgcctgagg caccgataac gccataaatt tgtccagctg gcacatgcag
    64561 gctgacgttg ttcaacgcct ggatggtgcg ggtgccctgg tggaacactt tggtgatatt
    64621 cgaaagtttt atcattgatt atttattatc gtcattaagt tagtcgtagc atctcgaatg
    64681 cctgaaacgg gcaacgccgt caatgaaatg gatgttaagg catccagacg tctaaatcaa
    64741 tcaggtttat gcgaagagca ctttcttgca ggtcgaaaca tgcgatacta gcgtcccatg
    64801 ccttattaag gagctataaa aggtggcgaa gagcgtaccc gcaatttttc ttgaccgtga
    64861 tggcaccatt aatgtcgatc acggctatgt ccatgagatc gacaactttg aatttatcga
    64921 cggtgttatt gacgccatgc gcgagctaaa aaaaatgggc tttgcgctgg tggtagtaac
    64981 caaccagtct ggcattgctc gcggtaaatt taccgaagca cagtttgaaa cgctgaccga
    65041 gtggatggac tggtcgctgg cggaccgaga tgtcgatctg gatggtatct attattgccc
    65101 gcatcatccg caggggagtg ttgaagagtt tcgccaggtc tgcgattgcc gcaaaccaca
    65161 tccggggatg cttttgtcag cacgcgatta tttgcatatt gatatggccg cttcttatat
    65221 ggtgggcgat aaattagaag atatgcaggc agcggttgcg gcgaacgtgg gaacaaaagt
    65281 gctggtgcgt acgggtaaac ctattacacc tgaagcagaa aacgcggcag attgggtgtt
    65341 aaatagcctg gcagacctgc cgcaagcgat aaaaaagcag caaaaaccgg cgcaatgatt
    65401 aaaagatgaa cggttgaaat aaaaatgcat ttttccgctt gtcttcctga gccgactccc
    65461 tataatgcgc ctccatcgac acggcggatg tgaatcactt cacacaaaca gccggttcgg
    65521 ttgaagagaa aaaatcctga aattcagggt tgactctgaa agaggaaagt gtgatatacg
    65581 ccacctcgcg acagtgagct gaaagctgcg tcgcaactgc tctttaacaa tttatcagac
    65641 aatctgtgtg ggcactcgaa gatacggatt cttaacgtcg caagacgata aatgaatacc
    65701 aagtctcaag agtgaacacg taattcatta cgaagtttaa ttctttgagc atc
//
LOCUS       DAJTQY010000010        65461 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.010, whole genome shotgun sequence.
ACCESSION   DAJTQY010000010 DAJTQY010000000
VERSION     DAJTQY010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 65461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65461
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.010"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            121..564
                     /locus_tag="OO349_000653"
     CDS             121..564
                     /locus_tag="OO349_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004997737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5865844.1"
                     /translation="MDLALVTGQRKGDLAAMRWEDIRDSRLYVEQQKTGAKIRISLPT
                     TISRLNLTLADVLDNLKKINGKNEKLLGGKTARTIAAQFRIARDTSGLKWEGDPPPFH
                     EIRSLSGRLHSAEKGSDFTQALLGHRSSSMTDKYRDGRGREWKDI"
     gene            800..1618
                     /gene="ybhA"
                     /locus_tag="OO349_000654"
     CDS             800..1618
                     /gene="ybhA"
                     /locus_tag="OO349_000654"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HCR5865845.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETTQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIAHGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(1619..2677)
                     /gene="modC"
                     /locus_tag="OO349_000655"
     CDS             complement(1619..2677)
                     /gene="modC"
                     /locus_tag="OO349_000655"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HCR5865846.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLGEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(2680..3369)
                     /gene="modB"
                     /locus_tag="OO349_000656"
     CDS             complement(2680..3369)
                     /gene="modB"
                     /locus_tag="OO349_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HCR5865847.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPSETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(3369..4142)
                     /gene="modA"
                     /locus_tag="OO349_000657"
     CDS             complement(3369..4142)
                     /gene="modA"
                     /locus_tag="OO349_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5865848.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(4309..4458)
                     /gene="acrZ"
                     /locus_tag="OO349_000658"
     CDS             complement(4309..4458)
                     /gene="acrZ"
                     /locus_tag="OO349_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HCR5865849.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            4587..5375
                     /gene="modE"
                     /locus_tag="OO349_000659"
     CDS             4587..5375
                     /gene="modE"
                     /locus_tag="OO349_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HCR5865850.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            5443..6915
                     /gene="modF"
                     /locus_tag="OO349_000660"
     CDS             5443..6915
                     /gene="modF"
                     /locus_tag="OO349_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HCR5865851.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPSEDYTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDKIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANESRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSSETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQVVSDRQQKLVQQWLDILGIDK
                     HTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            7176..8192
                     /gene="galE"
                     /locus_tag="OO349_000661"
     CDS             7176..8192
                     /gene="galE"
                     /locus_tag="OO349_000661"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HCR5865852.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVAESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPQIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            8202..9248
                     /gene="galT"
                     /locus_tag="OO349_000662"
     CDS             8202..9248
                     /gene="galT"
                     /locus_tag="OO349_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HCR5865853.1"
                     /translation="MTQFNLVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            9252..10400
                     /gene="galK"
                     /locus_tag="OO349_000663"
     CDS             9252..10400
                     /gene="galK"
                     /locus_tag="OO349_000663"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HCR5865854.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            10394..11434
                     /gene="galM"
                     /locus_tag="OO349_000664"
     CDS             10394..11434
                     /gene="galM"
                     /locus_tag="OO349_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HCR5865855.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            11637..12389
                     /gene="gpmA"
                     /locus_tag="OO349_000665"
     CDS             11637..12389
                     /gene="gpmA"
                     /locus_tag="OO349_000665"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HCR5865856.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(12556..13608)
                     /gene="aroG"
                     /locus_tag="OO349_000666"
     CDS             complement(12556..13608)
                     /gene="aroG"
                     /locus_tag="OO349_000666"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HCR5865857.1"
                     /translation="MNYQNDDLHIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLVNAVKARRG"
     gene            13924..14304
                     /locus_tag="OO349_000667"
     CDS             13924..14304
                     /locus_tag="OO349_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HCR5865858.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            14418..15359
                     /gene="zitB"
                     /locus_tag="OO349_000668"
     CDS             14418..15359
                     /gene="zitB"
                     /locus_tag="OO349_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HCR5865859.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHTTIQMEYQPCHRPDCHLNEGVSGHSHHHH"
     gene            complement(15356..16075)
                     /gene="pnuC"
                     /locus_tag="OO349_000669"
     CDS             complement(15356..16075)
                     /gene="pnuC"
                     /locus_tag="OO349_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HCR5865860.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(16113..17156)
                     /gene="nadA"
                     /locus_tag="OO349_000670"
     CDS             complement(16113..17156)
                     /gene="nadA"
                     /locus_tag="OO349_000670"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HCR5865861.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQVECSLDLGCPVEEFNAFYDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKALPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNDLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            complement(17405..17480)
                     /locus_tag="OO349_000671"
     tRNA            complement(17405..17480)
                     /locus_tag="OO349_000671"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17445..17447),aa:Lys,seq:ttt)
     gene            complement(17484..17559)
                     /locus_tag="OO349_000672"
     tRNA            complement(17484..17559)
                     /locus_tag="OO349_000672"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17524..17526),aa:Val,seq:tac)
     gene            complement(17611..17686)
                     /locus_tag="OO349_000673"
     tRNA            complement(17611..17686)
                     /locus_tag="OO349_000673"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17651..17653),aa:Lys,seq:ttt)
     gene            complement(17689..17764)
                     /locus_tag="OO349_000674"
     tRNA            complement(17689..17764)
                     /locus_tag="OO349_000674"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17729..17731),aa:Val,seq:tac)
     gene            complement(17901..17976)
                     /locus_tag="OO349_000675"
     tRNA            complement(17901..17976)
                     /locus_tag="OO349_000675"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17941..17943),aa:Lys,seq:ttt)
     gene            complement(18141..18932)
                     /gene="cpoB"
                     /locus_tag="OO349_000676"
     CDS             complement(18141..18932)
                     /gene="cpoB"
                     /locus_tag="OO349_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HCR5865862.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGATASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(18942..19463)
                     /gene="pal"
                     /locus_tag="OO349_000677"
     CDS             complement(18942..19463)
                     /gene="pal"
                     /locus_tag="OO349_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HCR5865863.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(19498..20790)
                     /gene="tolB"
                     /locus_tag="OO349_000678"
     CDS             complement(19498..20790)
                     /gene="tolB"
                     /locus_tag="OO349_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HCR5865864.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(20923..22164)
                     /gene="tolA"
                     /locus_tag="OO349_000679"
     CDS             complement(20923..22164)
                     /gene="tolA"
                     /locus_tag="OO349_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HCR5865865.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEVAAAKAAADAKAAEEAAKKAAADAK
                     KKAEAEAAKAAAEAQKKAEVAAAALKKKAEAAEAAAAEARKKAATEAAEKAKAEAEKK
                     AAAEKAAADKKAAAEKAAADKKAAEKAAADKAAADKKAAAEKAAADKKAAAAKAAAEK
                     AAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASGADINNYAG
                     QIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKI
                     PKPPSQAVYEVFKNAPLDFKP"
     gene            complement(22229..22657)
                     /gene="tolR"
                     /locus_tag="OO349_000680"
     CDS             complement(22229..22657)
                     /gene="tolR"
                     /locus_tag="OO349_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HCR5865866.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(22661..23353)
                     /gene="tolQ"
                     /locus_tag="OO349_000681"
     CDS             complement(22661..23353)
                     /gene="tolQ"
                     /locus_tag="OO349_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HCR5865867.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(23350..23754)
                     /gene="ybgC"
                     /locus_tag="OO349_000682"
     CDS             complement(23350..23754)
                     /gene="ybgC"
                     /locus_tag="OO349_000682"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCR5865868.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(23904..24197)
                     /gene="ybgE"
                     /locus_tag="OO349_000683"
     CDS             complement(23904..24197)
                     /gene="ybgE"
                     /locus_tag="OO349_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HCR5865869.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(24197..24310)
                     /gene="cydX"
                     /locus_tag="OO349_000684"
     CDS             complement(24197..24310)
                     /gene="cydX"
                     /locus_tag="OO349_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HCR5865870.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(24325..25464)
                     /gene="cydB"
                     /locus_tag="OO349_000685"
     CDS             complement(24325..25464)
                     /gene="cydB"
                     /locus_tag="OO349_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCR5865871.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLL
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(25480..27048)
                     /gene="cydA"
                     /locus_tag="OO349_000686"
     CDS             complement(25480..27048)
                     /gene="cydA"
                     /locus_tag="OO349_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HCR5865872.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            complement(27897..30530)
                     /gene="mngB"
                     /locus_tag="OO349_000687"
                     /pseudo
     CDS             complement(27897..30530)
                     /gene="mngB"
                     /locus_tag="OO349_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706502.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
     gene            complement(30548..32464)
                     /gene="mngA"
                     /locus_tag="OO349_000688"
     CDS             complement(30548..32464)
                     /gene="mngA"
                     /locus_tag="OO349_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HCR5865873.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LKEVYRRESLGPTALSEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPLNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQSLTLERGDETRTLQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLIRWMKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            32634..33356
                     /gene="mngR"
                     /locus_tag="OO349_000689"
     CDS             32634..33356
                     /gene="mngR"
                     /locus_tag="OO349_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HCR5865874.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITVQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            complement(33459..34328)
                     /gene="sucD"
                     /locus_tag="OO349_000690"
     CDS             complement(33459..34328)
                     /gene="sucD"
                     /locus_tag="OO349_000690"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HCR5865875.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGASASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(34328..35494)
                     /gene="sucC"
                     /locus_tag="OO349_000691"
     CDS             complement(34328..35494)
                     /gene="sucC"
                     /locus_tag="OO349_000691"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HCR5865876.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(35588..36805)
                     /gene="odhB"
                     /locus_tag="OO349_000692"
     CDS             complement(35588..36805)
                     /gene="odhB"
                     /locus_tag="OO349_000692"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HCR5865877.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEEPTRLLLDV"
     gene            complement(36820..39621)
                     /gene="sucA"
                     /locus_tag="OO349_000693"
     CDS             complement(36820..39621)
                     /gene="sucA"
                     /locus_tag="OO349_000693"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HCR5865878.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELISAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPNSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(39922..40638)
                     /gene="sdhB"
                     /locus_tag="OO349_000694"
     CDS             complement(39922..40638)
                     /gene="sdhB"
                     /locus_tag="OO349_000694"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HCR5865879.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(40654..42420)
                     /gene="sdhA"
                     /locus_tag="OO349_000695"
     CDS             complement(40654..42420)
                     /gene="sdhA"
                     /locus_tag="OO349_000695"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HCR5865880.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALHDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(42420..42767)
                     /gene="sdhD"
                     /locus_tag="OO349_000696"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(42420..42767)
                     /gene="sdhD"
                     /locus_tag="OO349_000696"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HCR5865881.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(42761..43165)
                     /gene="sdhC"
                     /locus_tag="OO349_000697"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(42761..43165)
                     /gene="sdhC"
                     /locus_tag="OO349_000697"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCR5865882.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILQWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            43546..43653
                     /locus_tag="OO349_000698"
     CDS             43546..43653
                     /locus_tag="OO349_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5865883.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            43859..45142
                     /gene="gltA"
                     /locus_tag="OO349_000699"
     CDS             43859..45142
                     /gene="gltA"
                     /locus_tag="OO349_000699"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HCR5865884.1"
                     /translation="MADTKAKLNLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSYGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            45533..46099
                     /locus_tag="OO349_000700"
     CDS             45533..46099
                     /locus_tag="OO349_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5865885.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLLLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            46150..48609
                     /locus_tag="OO349_000701"
     CDS             46150..48609
                     /locus_tag="OO349_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5865886.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNEQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNISIPQAWLAWHSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTAGINTGAWRLRSDYQLNHTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSPNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYSGLLLSGDD
                     YHSAAMGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            48624..49355
                     /locus_tag="OO349_000702"
     CDS             48624..49355
                     /locus_tag="OO349_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCR5865887.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            49352..50428
                     /locus_tag="OO349_000703"
                     /pseudo
     CDS             49352..50428
                     /locus_tag="OO349_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            50580..51626
                     /locus_tag="OO349_000704"
     CDS             50580..51626
                     /locus_tag="OO349_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCR5865888.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(51623..52414)
                     /gene="nei"
                     /locus_tag="OO349_000705"
     CDS             complement(51623..52414)
                     /gene="nei"
                     /locus_tag="OO349_000705"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HCR5865889.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFPNGLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(52450..53184)
                     /gene="pxpA"
                     /locus_tag="OO349_000706"
     CDS             complement(52450..53184)
                     /gene="pxpA"
                     /locus_tag="OO349_000706"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HCR5865890.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVAAQTVCLH
                     GDGEHALAFARRLRATFAKKGIVVAA"
     gene            complement(53174..54106)
                     /gene="pxpC"
                     /locus_tag="OO349_000707"
                     /pseudo
     CDS             complement(53174..54106)
                     /gene="pxpC"
                     /locus_tag="OO349_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
     gene            complement(54100..54756)
                     /gene="pxpB"
                     /locus_tag="OO349_000708"
     CDS             complement(54100..54756)
                     /gene="pxpB"
                     /locus_tag="OO349_000708"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HCR5865891.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAYCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGIC
                     "
     gene            complement(54779..55522)
                     /gene="ybgI"
                     /locus_tag="OO349_000709"
     CDS             complement(54779..55522)
                     /gene="ybgI"
                     /locus_tag="OO349_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HCR5865892.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGMELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            55793..57274
                     /gene="dtpD"
                     /locus_tag="OO349_000710"
     CDS             55793..57274
                     /gene="dtpD"
                     /locus_tag="OO349_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HCR5865893.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(57424..58842)
                     /gene="phrB"
                     /locus_tag="OO349_000711"
     CDS             complement(57424..58842)
                     /gene="phrB"
                     /locus_tag="OO349_000711"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HCR5865894.1"
                     /translation="MTTHLAWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWET
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVDVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIKPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCEN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHRPFIAWTDRVQWQSNPAHLQAWQKGKTGYPIIDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPITQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVKLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
     gene            complement(58839..59348)
                     /locus_tag="OO349_000712"
     CDS             complement(58839..59348)
                     /locus_tag="OO349_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HCR5865895.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYRQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            59477..59764
                     /locus_tag="OO349_000713"
     CDS             59477..59764
                     /locus_tag="OO349_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system SymE family toxin"
                     /protein_id="HCR5865896.1"
                     /translation="MAEHDITPEVTISKTQRRYKVGYVRARHQDRKTGIARYYSQHPS
                     LHLKGDWLKEAGFDTGRGVTVKISEGGLTIIADSDEVQELRDGMFSVLNES"
     gene            complement(59819..60199)
                     /locus_tag="OO349_000714"
     CDS             complement(59819..60199)
                     /locus_tag="OO349_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5865897.1"
                     /translation="MNNLNTLLSGKVLLKEISPLMPGYRTWIEISLIDELKPSYPFRL
                     DEYAMIGHSPYANECNKDEAKFKLRISSFLASDIDNEYDPSYDYVGKYEIIASLNELK
                     TRLSTLHVNLEQFINSSEDDEYPL"
     gene            complement(60202..64866)
                     /locus_tag="OO349_000715"
                     /pseudo
     CDS             complement(60202..64866)
                     /locus_tag="OO349_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390554.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
     gene            complement(64885..65088)
                     /locus_tag="OO349_000716"
                     /pseudo
     CDS             complement(64885..65088)
                     /locus_tag="OO349_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
BASE COUNT        15865 a        17731 c        15981 g        15884 t
ORIGIN      
        1 gatccagctt ctgcaaccag gccattcaga actgaagtga agcgcagtcg actgttaatt
       61 gacgagtatc tgttaatcag gaaaatagca gacgtccaga acgaatggtt cggcctttgc
      121 atggatcttg cactggtaac aggacagcgc aaaggagatc tggcggctat gagatgggag
      181 gatataagag atagcaggct atacgtggaa caacaaaaaa caggggcaaa aattcgcatt
      241 tctctcccca ccacgatttc cagattgaat ttaacacttg ccgatgtact ggataacttg
      301 aaaaaaataa acggtaaaaa cgaaaagtta ctcggaggga agacagccag gacaattgca
      361 gcacagttca ggattgcccg agacacatcc ggattgaaat gggaaggtga cccaccgcca
      421 tttcatgaaa tccgaagttt atccggtcgc ctgcactcag cagaaaaagg cagtgatttc
      481 actcaggcgc tccttggaca tcgttcatcc agcatgacgg ataaataccg tgacggacga
      541 gggagggaat ggaaagacat ttagaattaa tttcgaatga tttcgaaatg ccacttgtaa
      601 ttaattgatt ataaagaatt aattcctaaa gagcatttgc ttcatgaatg ctcctttgca
      661 tttagcaact gactgtaaca gctaaaatta gtcgcttttg gcggtgaatg ctgaattaaa
      721 acgcagaatt ttgtccgata gtggtatcgg tgtaccattc gcgcagacga aaattcttaa
      781 ccttaatctg gaatacgcca tgaccacacg cgtgattgct ctcgacttag acggcacctt
      841 attgaccccg aaaaagacct tgcttccatc atcgatagaa gccctggccc gcgctcgcga
      901 agcaggctat caattaatca tcgtcacagg tcgccatcac gtcgctattc atccttttta
      961 tcaggcgctg gcgctggata cacctgctat ttgctgtaat ggcacctatt tgtatgatta
     1021 tcatgcaaaa accgtgctgg aagcggaccc aatgcccgtt aataaagccc tgcaactcat
     1081 tgagatgctg aatgaacacc acattcacgg tctgatgtat gtcgatgatg caatggtcta
     1141 tgagcacccg accgggcatg tcattcgcac gtctaactgg gcgcaaaccc tgccgccgga
     1201 acagcgtccg actttcacac aagtcgcttc tctggctgaa acgacgcaac aagttaacgc
     1261 cgtatggaag ttcgccctga cgcacgatga cctgccgcaa ttgcagcatt ttggtaagca
     1321 tgtcgaacat gaactgggac tggagtgtga atggtcctgg cacgatcagg ttgatattgc
     1381 acacggcggc aacagcaaag gtaaacgttt gacgaaatgg gttgaggcgc aaggttggtc
     1441 gatggaaaac gtcgtggcat tcggcgataa ctttaatgat atcagtatgc tggaagccgc
     1501 tggtacaggc gtggcgatgg gcaacgccga tgacgcggta aaagcgcgcg ccaacattgt
     1561 gattggtgat aacaccaccg acagcattgc ccagttcatt tatagccacc tgatttaatc
     1621 aggcggttat cgacacactt ttaatttgcg cgtacagcca caggccaggt ttgatcgcca
     1681 gttcatccct ggcccacggg ctgatacgcg cccacagcgt tttaccgccg acttccagct
     1741 ccacttccac ctggccgttg tcgtcataac tattaacaac ttttgcccgc aatacgttac
     1801 gaatgctggt ttgctgcggc ggttgtaaaa ccagagaaac atcggaagcc tgaatgcgga
     1861 tacgtagcgc agcttgcagc ggttcgccca gcttattgac ccacaaatgc tgatcgccca
     1921 gcgccagcgc ggtcatcgcg taatgcggat gatgctcaag caccgtcact ttcagaatgc
     1981 tactttgttg ctctttcggc agccacggat tcatcacgct actgccccac acttcctcca
     2041 gcgcgccaaa ggctttcacc tgaccgtttt ccagtaccat cactctgtct gccagatgga
     2101 ggatctcatc cagcgaatgg ctgacataca acatcggaat gttgatttcc cgtgtcagcc
     2161 gttgcagata aggcaacagt tcgcgtttac gcggaatatc cagtgacgcc agcggttcat
     2221 ccagcaacag caattccggt gctgtcagca aagcccgacc aatcgccacg cgctgttttt
     2281 cgcctccgga caggctgcct ggtaaacggt caagcaacgg ttcaatgcct aaaagcgcca
     2341 ccagcttatc gaactgatcg accatacttt tcgacatgcc gtagcgcaga ttgccacgca
     2401 ctttgtaatg cgggaacaac cgcgcatcct gaaaaacata gccaacacga cgcttttccg
     2461 gcgtcaggca gatacctttt tcggcatcat ttagtacccg cccattgagg acaatccgcc
     2521 ctttttgcgg gcgcgtcagt ccactgatgg cgttaatcag cgaagttttt ccggcaccgg
     2581 agacgccaaa gatagcagtg atgccattgg cgggcagcgt ttcattaatg gtcaggcaat
     2641 ggttgcccaa cgtctgggaa aaattcagct ccagcatgat tagcgccccg cccgttcacg
     2701 gctgattctg gccagccatt ctgaaatcaa cagggagatc atcgccagcg caatagaaat
     2761 aatgcacagt ctcgccgcgc cactttcccc gccgggggtc tggatcaggg tatacatggc
     2821 agaaggaatg gttcgcgttt cgctaggaat gttcgacaca aaggtgatgg ttgcaccaaa
     2881 ctcaccgaga gaacgagcaa aagccagtac cgtaccaaca ataattcccg gtaaggtcag
     2941 cggtaacgtg atagtaaaga aaacgcgcca gcgcccggca cctagtgttc ttgcggcctg
     3001 ttccagtttg acatcaaccc cttccagcgc cagacgaatt gcccgcacca tcagcggaaa
     3061 cgacatgacg gcggcagcga gaaccgcgcc gcgccagcta aaggcgaagg taataccaaa
     3121 ccagtcatac agacgttcac cgataaatcc gcgccgtccc atcgaaacta ataataagta
     3181 accgacgacc acgggcggta acaccagcgg tagatgcagt acgctgtcga gcagagcttt
     3241 gcccggaaac gtgcaacgca ccagtaacca ggcaaaaaag atcccaaacg gcaggctaaa
     3301 cagcacagcc agggaagaaa ctttcaggct taataaaact gcctgccatt ctggatcggt
     3361 cagtatcatt acttggttgt aaatccgtaa cgtttaaaga tttccgctgc ctgcggtccc
     3421 ttcagataat cataaaatgc tttcactgtc gcattgttat gcccttccac aacagcaacc
     3481 ggatattcca cttttttatg tgaatcttcc gggaaggtgg caaccacttt tacccctttg
     3541 ctggcaactg cgtcagaacc gtagacaatg cccagaggcg cttcgttacg ttcgaccagc
     3601 gccagcgccc cacgaacatc ttccgctggg gccagtttcg gagagagcgt atcccatgcg
     3661 cccagttttt gcagtgcttc ttttgcataa atgccagcgg gaacatgttc cggatcgcca
     3721 accgccaggc gaccgccatt cagcagcgaa gtccagttag ttttgctgtc gatggtgaaa
     3781 tctttctgct cgctggcttt cggtgctaca acgaccaggc tattgccgag cagtgtctga
     3841 cgcgtagcag tatcgatcgc ttttttatca accgcataat ccatccattt ctgatcggca
     3901 gaaataaaca gatccgcagg ggcacccgct tcaatctgac gggcgagagt agatgacgaa
     3961 gcgaaagaag aaaccacatc cacgcctttc tcttttttat actgcgtagc aatgtcctgc
     4021 attgcgttag tcagtgatgc ggcggcgaac accgtgattt tcccttcatc tgccagtgca
     4081 ttgccagcaa ctgcgaaaga gagtgccgcc ccggcaaaca agttcaacca tttacgagcc
     4141 attggtaacc ccttaatgta acgttatgta ggcgacaata taacgactaa ctcggaattt
     4201 tcccagcagt tattgctaac ctttgtgagg tagataagaa aaaatatcgg caggaaaagc
     4261 aggaagttga gaaaaagaaa atgcccgact aagcgggcac tcagggaatc aatgattttg
     4321 tccgggctgg tcttttttac caacaccaga aaagatgttg aatacttcac caagaccgta
     4381 aatcagaccc aggatgatgg ccatcacgac aggtaccatg attacggcga ataccagact
     4441 ttttaataac tctaacatgg tcaactccag atatagtcat gagactattc taaccgctaa
     4501 gcacagaaaa gcactcccct tttgtgcggt cagctttgcg tggcgttcgt tttccgtcac
     4561 aataagactt ttgccaggac attgttatgc aggccgaaat ccttctcacc cttaagctcc
     4621 aacaaaaatt attcgccgac ccgcgccgca tttcgctact aaaacacatt gcgctttccg
     4681 gttccattag ccagggagcg aaagatgccg gtatcagcta taaaagcgcc tgggatgcca
     4741 ttaacgagat gaatcagtta agtgagcata ttctggtcga gcgcgcaaca ggcggtaaag
     4801 gtggcggagg cgcagtactg acccgctatg gtcagcgact gattcagctc tatgacttac
     4861 tggcgcaaat ccagcaaaaa gcctttgatg tgttaagtga cgatgacgcc ctgccgctga
     4921 acagcctgct ggccgcgatc tcacgttttt cactgcaaac cagcgcccgt aaccagtggt
     4981 tcggtaccat caccgcccgc gatcatgatg acgttcaaca gcatgttgat gtcttactgg
     5041 ctgacggaaa aacacgcctg aaagtcgcaa ttaccgcaca aagcggcgca cgtctggggc
     5101 tggatgaagg caaagaagtg ttgatattgc taaaagcgcc gtgggtaggc attactcagg
     5161 acgaagcggt cgcacaaaac gccgacaacc aattaccggg tattattagt catattgagc
     5221 gcggcgcaga gcagtgcgaa gtattaatgg cgctacccga cgggcaaaca ctgtgcgcca
     5281 cagtgccggt aaatgaagcg acttctcttc agcaagggca gaatgtcacg gcctacttta
     5341 atgccgacag cgtgattatc gccacgctgt gctaagcgtg ttgacaattt gttatgaaac
     5401 acgtatccct gtcagtaatc gctgcacaaa atgggatata aaatgtcatc gttgcaaatt
     5461 ttgcaaggca cgtttcgtct tagcgacaca aaaacgctgc aattgcctca gctaacgtta
     5521 aacgcgggtg atagttgggc gtttgtcggt tcgaatggaa gtggaaagtc ggcgctggcc
     5581 cgcgcgctgg cgggggaact tccgcttttg aaaggtgaac ggcaaagcca gttttcccac
     5641 atcactcgtc tctccttcga gcaattgcaa aagctcgtca gcgacgaatg gcaacggaat
     5701 aacaccgata tgctcggccc tagcgaagat tacaccggac gcactacggc tgagattatt
     5761 caggatgaag taaaggatgc accgcgttgc atgcaactgg cgcagcagtt cggtattacc
     5821 gccctcctcg accgacgctt taaatacctt tccactggcg agacgcgaaa aaccctgctg
     5881 tgtcaggcgc tgatgtcgga gcctgacttg ttgattcttg atgagccgtt cgatggcctg
     5941 gatgttgcct cacgtcagca gctggctgag cgactcgcct cgttacatca gtccggtatt
     6001 actttggtac tggtgctcaa tcgcttcgat aagatcccgg aatttgtcca gtttgctggc
     6061 gtgctggcgg attgtacgtt agcggaaact ggcgctaaag aggaactgct ccagcaagca
     6121 ctcgtcgcgc aacttgcgca tagtgaacag cttgaaggtg tgcaactgcc ggagccggat
     6181 gaaccttcag cacgtcacgc cttacccgcc aacgaatcgc gcattgtgct gaacaatggc
     6241 gtggtttctt ataatgatcg ccccattctt aataacctta gctggcaggt gaatccaggc
     6301 gaacactggc aaattgtcgg gccaaatggc gcgggaaaat cgacgttatt aagcctgatt
     6361 actggcgatc atccgcaagg ttacagcaac gatttgacgc ttttcggccg acgtcgcggc
     6421 agcagcgaaa ccatctggga catcaaaaag catatcggtt acgtcagcag tagtttgcat
     6481 ctggattacc gggtcagcac taccgtgcgt aatgtgattc tttctggcta ttttgattcg
     6541 attggcattt atcaggtcgt ttcggatcgc cagcaaaaac tggtgcagca gtggctggat
     6601 attctcggca ttgataaaca cacggctgac gctccgttcc atagtctttc ctggggacag
     6661 cagcgtctgg cgctgatcgt ccgcgcactg gtgaaacatc cgacgttgct tattctcgat
     6721 gaaccactac aggggcttga tccgctcaat cgccaactta tccgccgttt tgttgatgtg
     6781 ctgattagcg aaggtgaaac gcaattgttg tttgtttcgc accacgctga agatgcgcct
     6841 gcctgtatta cccatcgcct tgagttcgtg ccggacggtg gactctatcg ctatgtgctg
     6901 acaaaaatat attgagtcgg tagtgctgac cttgccggag gcggcctaag cgcactctcc
     6961 ggccaacggt tcgacgcatg caggcatgaa accgcttctt ttttcagata aaaagcgcaa
     7021 tcattcataa accctctgtt ttataatcac ttaatcgcgc ataaaaaacg gctaaattct
     7081 tgtgtaaacg attccactaa tttattccat gtcacacttt tcgcatcttt gttatgctat
     7141 ggttatttca taccacaagc ctaatggagc gaattatgag agttctggtt accggtggta
     7201 gcggttacat tggaagtcat acctgcgtgc aattactgca aaacggtcat gatgtcatca
     7261 ttcttgataa cctctgtaac agtaagcgca gcgtactgcc tgttatcgag cgtttaggcg
     7321 gcaaacatcc aacgtttgtt gaaggcgata ttcgtaacga agcgttgatg accgagatcc
     7381 tgcacgatca cgctatcgac accgtgatcc acttcgccgg gctgaaagcc gtcgccgaat
     7441 cggtacaaaa accgctggaa tattacgaca acaatgtcaa cggcactctg cgcctgatta
     7501 gcgccatgcg cgccgctaac gtcaaaaact ttatttttag ctcctccgcc accgtttatg
     7561 gcgatcagcc ccaaattcca tacgttgaaa gcttcccgac cggcacaccg caaagccctt
     7621 atggcaaaag caaattgatg gtggaacaga tcctcaccga cctgcaaaaa gcccagccgg
     7681 actggagcat tgccctgctg cgctacttca acccggttgg cgcgcatccg tcgggcgata
     7741 tgggcgaaga tccgcaaggc attccgaata acctgatgcc atacatcgcc caagttgctg
     7801 taggccgtcg cgactcgctg gcgatttttg gtaacgatta tccgaccgaa gatggtactg
     7861 gcgtacgcga ttacatccac gtaatggatc tggcggacgg tcacgtcgtg gcgatggaaa
     7921 aactggcgaa caagccaggc gtacacatct acaacctcgg tgctggcgta ggcagcagcg
     7981 tgctggacgt ggttaatgcc ttcagcaaag cctgcggcaa accggttaac tatcattttg
     8041 caccgcgtcg cgaaggcgac cttccggcct actgggcgga cgccagcaaa gccgaccgtg
     8101 aactgaactg gcgcgtaacg cgcacactcg atgaaatggc gcaggacacc tggcactggc
     8161 agtcacgcca tccacaggga tatcccgatt aaggaacgac catgacgcaa tttaatctcg
     8221 ttgatcatcc acatcgccgc tacaacccgc tcaccgggca atggattctg gtttcaccgc
     8281 accgcgctaa gcgcccctgg cagggggcgc aggaaacgcc agccaaacag gtgttacctg
     8341 cgcacgatcc agattgcttc ctctgcgcag gtaatgtgcg ggtgacaggc gataaaaacc
     8401 ccgattacac cgggacttac gttttcacta atgactttgc ggccttgatg tctgacacgc
     8461 cagatgcgcc agaaagcaac gatcctttga tgcgttgcca gagcgcgcgc ggtaccagcc
     8521 gggtgatctg cttttcaccg gatcacagta aaacgttgcc agaactgagc gttgcggcat
     8581 tgacggaaat cgtcaaaacc tggcaggagc aaaccgcaga gctgggaaaa acgtacccgt
     8641 gggtgcaggt ctttgaaaac aaaggtgcgg cgatgggctg ctctaacccg catccgcacg
     8701 gacagatttg ggcaaatagc ttcctgccta acgaagctga gcgcgaagac cgcctgcaaa
     8761 aagaatattt tgccgaacag aaatcaccaa tgctggtgga ttatgttcag cgcgagctgg
     8821 cagacggtag ccgtaccgtt gtcgaaaccg aacactggtt agccgttgtg ccttactggg
     8881 ctgcctggcc gttcgaaacg ctactgctgc ccaaagccca cgttttgcgg atcaccgatt
     8941 tgaccgacgc ccagcgcagc gatttggcgc tggcgttgaa aaagctgacc agtcgttatg
     9001 acaacctctt ccagtgctcc ttcccctact ctatgggctg gcacggcgca ccatttaatg
     9061 gcgaagagaa tcaacactgg cagctgcacg cgcactttta tccgcctctg ctgcgctccg
     9121 ccaccgtacg taaatttatg gttggttatg aaatgctggc agagacccag cgagacctga
     9181 ccgcagaaca ggcagcagag cgtttgcgcg cagtcagcga tatccatttt cgcgaatccg
     9241 gagtgtaaga aatgagtctg aaagaaaaaa cacaatctct gtttgccaac gcatttggct
     9301 accctgccac tcacaccatt caggcgcctg gccgcgtgaa tttgattggt gaacacaccg
     9361 actacaacga cggtttcgtt ctgccctgcg cgattgatta tcaaaccgtg atcagctgtg
     9421 caccacgcga tgaccgtaaa gttcgcgtga tggcagccga ttatgaaaat cagctcgacg
     9481 agttttccct caatgcgccc attgtcgcgc atgaaaacta tcaatgggcg aactacgttc
     9541 gtggcgtggt gaaacatctg caactgcgta acaacagctt cggcggtgtg gacatggtga
     9601 tcagcggcaa tgtgccgcag ggtgccgggt taagttcttc cgcttcactg gaagtcgcgg
     9661 tcggaaccgt attgcagcag ctttatcatc tgccgctgga cggcgcacaa atcgcgctta
     9721 acggtcagga agcagaaaac cagtttgttg gctgtaactg cgggatcatg gatcagctaa
     9781 tttccgcact cggcaagaaa gatcatgcct tgctgattga ctgccgctca ctggggacta
     9841 aagcagtttc catgccgaaa ggtgtggctg tcgtcatcat caacagtaac ttcaaacgta
     9901 ccctggttgg cagcgaatac aacacccgtc gtgaacagtg cgaaaccggt gcgcgtttct
     9961 tccagcagcc agctctgcgc gatgtcacca ttgaagagtt caacgctgtt gcacatgagc
    10021 tggacccaat cgtggcgaaa cgcgtgcgtc atatcctgac tgaaaacgcc cgcaccgttg
    10081 aagctgccag cgcgctggag caaggcgacc tgaaacgtat gggcgagttg atggccgagt
    10141 ctcatgcctc tatgcgcgat gatttcgaaa tcaccgtgcc gcaaattgac actctggtag
    10201 aaatcgtcaa agctgtgatt ggcgacaaag gtggcgtacg catgaccggc ggcggatttg
    10261 gcggctgtat cgtcgcgctg atcccggaag agctggtgcc tgccgtacag caagctgtcg
    10321 ctgaacaata tgaagcaaaa acaggtatta aagagacttt ttacgtttgt aaaccatcac
    10381 aaggagcagg acagtgctga acgaaactcc cgcactggca cccgatggtc agccgtaccg
    10441 actgttaact ttgcgtaaca acgcagggat ggtagtcacg ctgatggact ggggtgcgac
    10501 tttactttcc gcccgtattc cgctttccga tggcagcgtc cgcgaggcgc tgctcggctg
    10561 tgccagtccg gaatgctatc aggatcaggc tgcgtttctg ggggcctcta ttggtcgtta
    10621 tgccaaccgc atcgccaata gtcgctatac ctttgacggt gaaaccgtga cgctttcgcc
    10681 aagccagggc gttaaccagc tgcacggcgg cccggaaggg ttcgacaaac gtcgctggca
    10741 gattgtgaac cagaacgatc gtcaggtgct gtttgccctg agttcagatg atggcgatca
    10801 gggcttcccg ggtaatctcg gcgcgacggt gcaatatcgt ctgaccgacg ataaccgtat
    10861 ctccattact tatcgcgcca cagttgataa accttgcccg gtgaatatga ctaatcacgt
    10921 ctatttcaat cttgacggcg agcagtctga cgtgcgcaat cacaagttgc agattctggc
    10981 ggacgaatat cttccggttg atgaaggcgg cattccgcac gacggcttga aatctgtcgc
    11041 cggaacgtct tttgatttcc gcagcgccaa aatcatcgcc agtgagtttc ttgccgacga
    11101 cgatcagcgc aaagtgaaag gttacgatca cgcattcttg ttacaggcca aaggcgatgg
    11161 caagaaagtg gcggcgcatg tctggtcagc agatgaaaaa ttgcagctga aggtctacac
    11221 caccgctccg gctcttcaat tctactccgg caacttcctc ggcggcacac cgtcgcgggg
    11281 aaccgaacct tacgccgact ggcaagggct ggcgttggaa agcgagtttc tgccggacag
    11341 cccgaaccac cctgaatggc cgcaaccgga ctgcttcctg cgtcctggcg aagagtattc
    11401 cagcctgacg gaatatcagt ttattgctga gtaatgttaa gccctcgtca tgagggcttt
    11461 atcttcatat tgttcaaatc accagcaaac accgacatat ttgcaactca atattcacaa
    11521 caaccttaca ctgcgccact attttcgcta tggttatgcg taagcattgc tgttgcttcg
    11581 tcacggcaat ataatgagaa ttattatcat taaaagatga tttgaggagt aagcatatgg
    11641 ctgtaactaa gctggttctg gttcgtcatg gcgaaagtca gtggaacaaa gaaaaccgtt
    11701 tcaccggttg gtacgacgtg gatctgtctg agaaaggcgt aagcgaagca aaagcagcag
    11761 gtaagctgct gaaagaggaa ggttacagct ttgactttgc ttacacttct gtgctgaaac
    11821 gcgctatcca taccctgtgg aatgtgctgg acgaactgga tcaggcatgg ctgcccgttg
    11881 agaaatcctg gaaactgaac gaacgtcact acggtgcgtt gcagggtctg aacaaagcgg
    11941 aaactgctga aaagtatggc gacgagcagg tgaaacagtg gcgtcgtggt tttgcagtga
    12001 ctccgccgga actgactaaa gatgatgagc gttatccggg tcacgatccg cgttacgcga
    12061 aactgagcga gaaagaattg ccgctgacgg aaagcctggc gctgaccatt gaccgcgtga
    12121 tcccttactg gaatgaaacc attctgccgc gtatgaagag cggtgagcgc gtaatcatcg
    12181 ccgcacacgg taactccctg cgtgcgctgg tgaaatatct tgataacatg agcgaagaag
    12241 agattcttga gctgaatatc ccgactggcg tgccgctggt gtatgagttc gacgagaatt
    12301 tcaaaccgct gaaacgctat tatctgggta atgctgacga gatcgcggcg aaagcagcag
    12361 ccgttgcgaa ccagggtaaa gcgaagtaaa cgtcattcgt ttaaaaaatg agaaagccga
    12421 ctgcaagtga gtcggctttt ttgtttgtcg ttgctaacaa tggagcacat tgcctgatgc
    12481 gacgctgcgc gtcttatcag gcctgtggtg attcatcgga tacgccactc tgacggcgca
    12541 tccgacaatt aaaccttacc cgcgacgcgc ttttactgca ttcaccagtt gacgtaacag
    12601 agcatcggta tcttcccagc caatgcaggc atcggtgatg ctcttaccgt aggccagcgg
    12661 ctccccgcta tcgaggctct gattgccttc caccagatgg ctttccacca tcacgccaat
    12721 aatggccttt tcgccaccgg caatctgctg gcaaacgtca gcacaaacat ccatctgctt
    12781 tttgaattgt ttggacgaat tagcatggct gaaatctatc atcacctgag ctggcagacc
    12841 tgctttgttc agcccttctt tcacttcagc aacgtgcttc gcgctgtagt taggctcttt
    12901 accgccgcgc agaatgatat ggcaatcgcc gttaccactg gtattcacaa tcgccgaatg
    12961 cccccattta gttacggaca ggaagcagtg cggcgcaccg gcggcattaa tggcatcgat
    13021 agccacttta atcgtaccgt ctgtgccatt tttaaaacct accggacaag aaagaccaga
    13081 cgccagttcg cggtgcacct gcgattccgt ggtacgtgca ccaattgcgc cccagctcat
    13141 caggtcagcg agatactgag gagtgatcat atcgagaaac tcacccgccg ctggcagacc
    13201 gctgtcgtta atatcaagca gcaatttacg ggctatacgc agaccgtcgt tgatctggaa
    13261 gctgttatcc atatgcggat cgttaatcaa ccctttccag cccaccgtgg tacgcggctt
    13321 ttcaaaatag acgcgcatta cgatttccag ctcatctttc agctcttcac gcagcgccag
    13381 caagcgagtg gcatactctt tagccgcgac aggatcatga attgagcacg ggccaatcac
    13441 caccaacagg cgatcatcat tacctttcag gatcttatgg atcgcttttc gggcatgggc
    13501 aaccgtattc gcggcatttt cagttgcggg gaatttttcc agcaatgcga caggaggaag
    13561 taactctttg atttctttga tgtgtaaatc gtcgttctga taattcatgt ctgttccagt
    13621 gttgccatac ttatcttagt gaatgcaata cttccaatct atatcttccg tcagaatgtg
    13681 taaacggggt tttacactat gaacggatta atcctggaat tagcaagaaa aacgccagat
    13741 tgtcgcgaaa aacgagatct ctcctacaat ttctaactgt aactcctttg tttgttaatt
    13801 atttcaagat tctctgctgc gtttcataaa ctggctgaaa tcttaaacca atgccttata
    13861 ttcacctgca aatgcactgt tggaagaggt tatccgacat aacgaccata acaggagcat
    13921 cctatgaaaa tgacaaaact ggccacatta tttctgactg ccactctaag ccttgccagc
    13981 ggtgccgcac tggctgccga tagcggagcg caaactaata acggccaggc aaacgccgca
    14041 gctgatgcgg gccaggtagc ccctgacgcc cgtgaaaatg tcgcgccaaa taacgtcgac
    14101 aataacgggg taaataccgg ttctggcggc acaatgctgc attcggatgg ttcttcaatg
    14161 aacaatgacg gaatgaccaa agatgaagag cacaagaaca ccatgtgcaa agatggtcga
    14221 tgtccggata tcaataaaaa ggtacaaacc ggtgatggca tcaataatga tgtcgatacc
    14281 aaaaccgacg gtaccacgca gtaaccctgc aaaatagcca ataatgggag agcggagtct
    14341 ctccctttta atttttcact tctaaaagca gtatgatgtc ataaagttaa gtgagaataa
    14401 ttaagggatg actcgtcatg gcgcactcac actcacacac gtcctcacac ctgccagaag
    14461 ataataatgc tcgtcgcttg ttgtatgctt tcggcgtgac tgccgggttt atgctggtag
    14521 aagtcgttgg tggttttctt tctggttctc tggcattgct ggccgatgcg ggtcatatgt
    14581 tgaccgatac tgccgccctg cttttcgccc tactcgccgt gcaattttca cgtcgtcctc
    14641 ccaccattcg ccacactttc ggttggctaa gactgaccac gctcgcggcg tttgtgaatg
    14701 ccatcgccct cgtggtaatt accattttga ttgtctggga ggcgatagaa cgcttccgca
    14761 cgccgcgtcc ggtcgaggga ggcatgatga tggcaattgc cgtggctggg ttgctggcaa
    14821 atatactttc tttctggtta cttcatcacg gcagcgaaga gaaaaacctc aacgtacgag
    14881 cagcggcact gcatgtgctc ggggacctgc tgggttcggt tggggctatt attgccgcgt
    14941 tgattattat ctggactggc tggacacctg ctgaccccat tctctcgata ctggtgtcgc
    15001 ttctggttct gcgtagcgcg tggcgactgt tgaaagatag cgtgaatgaa ttacttgaag
    15061 gtgcaccggt atcgctggat atcgctgaac tgaagcgtcg tatgtgccgg gaaatcccgg
    15121 aagtccgcaa tgtgcaccat gtacatgtat ggatggtagg cgagaagccg gtgatgacgt
    15181 tgcatgtgca ggtgatcccg ccacacgatc acgatgcctt gttggatcag atccaacact
    15241 acctgatgga tcactatcag attgagcaca ccaccattca gatggaatat caaccttgtc
    15301 ataggccaga ctgccacctg aatgaagggg tgtctggcca ttcacatcat caccattaat
    15361 gggacagcgc gcgtgagcct ctttcacgtg cgctgttgat ccacatccgg ctgccgttga
    15421 gcgcaataaa ggtcaggatg atgtactcca gagacatggc gtaaacgccc tgaagtgcaa
    15481 aaataacgac gctaatcacg ttaataatca cccacaacag ccagttttcc acatacttac
    15541 gcgtcatcag aatcattgcc acgatagata acaccatcat gcatgaatcc cagaacggga
    15601 aagcgtccgg ttgcagttca ggcatcacaa cctgtaatcc taatgcttgc atgatcatga
    15661 ctgccacgcg ggtcaaaaat gcaaacaccg gattgataaa taccgtcatc agaccaatcg
    15721 aaacaacgca aaccgccaac cagctgagtg ccttcggcaa tggcaaccag cgaattttca
    15781 actccgcctc gttctgactg gtttgtcgcg accacgcata ccagccgtaa atattcgcgg
    15841 caaagaaaaa cacctgtaat agcaggctgg catacagctg aatctgaaag aaaataatgc
    15901 caaacaaggt aacgttgatc aggccaaaga agtagttgct gatcttctcc agactggcaa
    15961 ggccaataca cagcaacccg gcgatcgtgc ctaccgcttc gatccatgag agatcataac
    16021 cgcccgcccc tattggtata tgtaccagga tattctgcac actaaaaaaa tccatctttc
    16081 ccccaaagcg taacgttacg ccttattatt cgttatccac gtagtgtagc cgcaaaatcc
    16141 agcatacgat tgagcggcac cagcgccctc tctcgcagcc tctcatcgac atgaacctcg
    16201 tgattacttc cttcctgttc taatgcctct gcgatagcct gaaggtcatt catggccatc
    16261 cacggacaat gcgcgcagct gcggcaggtt gcgccctcac ccgcggttgg tgcttccagt
    16321 aactctttat ctggcaccgc ctgctgcatt ttgtagaaaa taccccgatc ggttgccaca
    16381 ataagcctct gatgaggcaa tgcttttgca gcagcgatca gttgactggt ggaaccgacc
    16441 gcatccgcca tatcgacaat agcttgtggt gattctggat gcaccagtat ggcagcatcc
    16501 gggtattctt cttgcaagcg ggttaacgcc tgagtcttaa attcatcatg cacaatgcag
    16561 gcaccctgcc agcataaaat gtctccaccc gtctgttttt gcacgtaacg ccccagatgt
    16621 ttgtcgggtg cccagatgat tttttcaccc aaactatcaa gatgatcaat aagttcgacg
    16681 gcaatgcttg aagttaccac ccaatctgcg cgcgctttta ccgcagcaga agtgttggcg
    16741 tagacgacga cagtacgatc gggatgggca tcgtaaaatg cgttaaattc ttcaacaggg
    16801 cagccgagat ccagtgaaca ctcaacctga agtgtcggca tcagaattgt tttttccgga
    16861 ctgagaattt tggcggtttc tcccataaat ctcaccccag cgactaacaa agtagaagcg
    16921 ggatgctttg caccgaagcg cgccatttcc agagaatcag aaatacagcc accggtttct
    16981 tctgccagtt gttgaatttc gggatcggta tagtagtggg caaccatcac cgcattacgt
    17041 tcttttagca aacgttttat cttctcgcgg taatacgctt tttcatcaat gcttaacggc
    17101 gtcggcttcg gggggaaagg ataaatcgcc gtgtctggat caaacattac gctcatctta
    17161 ccatctcgtt ttacaggctt aacgttaaaa ccgacattag cgtaacattc gctgttatcg
    17221 gtgtaatttg ttttatatgc taaacaagat agtcgaatgg tggggaaaag tcactcgaat
    17281 tacgcctaat tccaccgacg tagagttgct tgcacagtaa gctctgacga tgtcatcagg
    17341 tttacaaagt gaaattgagg atgcctgaat agcaaaaaag cgcctttagg gcgctttttt
    17401 acattggtgg gtcgtgcagg attcgaacct gcgaccaatt gattaaaagt caactgctct
    17461 accaactgag ctaacgaccc gagtggtggg tgatgacggg atcgaaccgc cgaccccctc
    17521 cttgtaaggg aggtgctctc ccagctgagc taatcacccg atactgcgct ggatactaca
    17581 aactacttcc accgaaccca ccgtaattag tggtgggtcg tgcaggattc gaacctgcga
    17641 ccaattgatt aaaagtcaac tgctctacca actgagctaa cgacccggtg gtgggtgatg
    17701 acgggatcga accgccgacc ccctccttgt aagggaggtg ctctcccagc tgagctaatc
    17761 acccgacact acgctggata ctacaactaa ctatgttagt ggtgggtcgt gcaggatgac
    17821 tcgacttcgt ctcgcccttc ggcgccgttg ctgatgcaac gttatccttc acgttttact
    17881 accgcttcca ccttagcgat tggtgggtcg tgcaggattc gaacctgcga ccaattgatt
    17941 aaaagtcaac tgctctacca actgagctaa cgacccactt tttcgttgct ttcggtttgt
    18001 ttgatatccc gtggcaacgg cggcatatat tactgatttc agacttgagc gcaacaaaaa
    18061 tttcgatgca gatcactcaa ctgcttatga ttcgcacgac acgaccagaa ataatgcgac
    18121 ttctggtcgt gtgttatgca ttacatcgcg ttcagacgtt tttgtgcctg tttagcgcca
    18181 tcagtaccag ggtatttact gataacctgc tggtatacgg ctttcgcttt tgcggtgtca
    18241 cctttgtcct gcatgatgac gccgacttta aacatcgcat cagcagcctt tggtgacttc
    18301 ggatagtttt tcactaccga agcaaaatag tacgccgcat catctttttt acccttgttg
    18361 tagtttaact gacccagcca ataattggcg tttggcaggt aagttgaatc agggtaattt
    18421 ttgatgaaat tctgaaatgc caccattgcg tcatcctggc gggatttatc ctgcaccagc
    18481 gcaatagctg cattgtaatc cgtgtttgca tcgccgcttt tcaccggcgc gccagcattc
    18541 gcagtaccag catcagctgt cggcgtcgtt gatgccgtcg caccgctttg atcgctgctg
    18601 gttgattgcg ccgctgcacc accgctgctg aggctgtcga tctgcaacag gatctgcttt
    18661 tgccgctcca cgacctgatt cagttgatac tgattttcct gaatctgacc acgcagggaa
    18721 tcaatatcgg attgattatc agaaagttgt tgctggagtt gggttaaaag ctggctgtga
    18781 gcattagaaa tacgctcaag ttgagtgacg cggtcttcga ccgagcctga gccgacacta
    18841 ctgattggtg cctgagcaaa agcggcccag ggggccgcta taccaaccag taacgacaga
    18901 ctcaatagtt gatgtctgaa gttactgctc atgcaattct cttagtaaac cagtaccgca
    18961 cgacggtttt tggagtatgc cgcttcgtca tgacccagta ctgcaggttt ttctttaccg
    19021 taagaaacga tggagatctg gtctgcagaa acgcctttac cctgcaggta catcttaacg
    19081 gcgttcgcac gacgttcacc cagggagatg ttgtattccg gagtaccacg ttcgtccgcg
    19141 tgaccttcta cggtgacttt gtaagacggg ttgctacgca ggaagtttgc atgtgcatcc
    19201 agcatttgag cgaagtcaga acggatatcg tacttgtcca gatcgaagta aacgatgttg
    19261 ttctgctgca gctgttgcat ttgcagacga gcctgctctt cggaagacat gttgccgttg
    19321 ccgccgttcg catccatacc agtgccggca cccagcatgc cttcgctgcc gtcattgctg
    19381 gcgttcttgt tggaagaaca tgccgcaatt gccataacag gcagagcaat catcagccct
    19441 ttcagcactt tgttcagttg catttcaatg attcctttac tattcaatta attattatca
    19501 cagatacggc gaccaggcag ggaatttgac ctgtccatca gttgccggaa gacgcgcttt
    19561 gaaacgccca tctgtagaaa ccaaattcag cacggatccc atcccctgag aagagctgta
    19621 gattaccata gtgccgttag gtgccagact tggcgtttca tccaggaacg tggacgacag
    19681 aacttgtacg cctcccgttg ccagatcttg tttggcaatg tgctgctgcc caccattgga
    19741 gctgaccatt accataaatt taccgtcgct gctgacatcc gcatcctggt tctgcgaacc
    19801 ttcccaggta atacgttgtg gcgcaccgcc gttgatattc actttataaa cctgcggacg
    19861 accggcctgg tcagaagtaa atgccaggtt ctggctatcc gggaaccagg tcggttcggt
    19921 attgttactg cgaccatcag tcacctggcg gatctgacca gaagccaaat ccattacgta
    19981 caggttcaga ctaccggttt tcgacaaggc gaatgccagt ttgctgccgt ctggcgagaa
    20041 tgcaggtgca ccgttgtgac gcgggaatga agccacctga cgtacagcgc catttgccag
    20101 cgtctgaata accagcgcgg aacgaccgct ttcgaaggtc acataagcca gtttagaacc
    20161 gtctggtgac cacgccggag acatcagcgg ctgcggtgaa cggtgaacga caaactggtt
    20221 gtaaccgtca tagtcagata cgcgcagttc atacgggaac tgaccgccgt tggtctgaac
    20281 aacgtaggca atacgggtac ggaacgcacc tttaatgccg gtcagttttt caaacacttc
    20341 atcactggcg gtatgaccag cataacgcag ccactgcttg ttcactttgt acgagttctg
    20401 agcaagtaca gtacccggtg cgccgccagt gtcaacaagt tgataagcaa cattgtaaga
    20461 accatccgga ttcggagtga cctgaccgac aactacagcg tcaatgccca gtgcggacca
    20521 tgcagctggt tgtacttcct gcgcactacc cggctgctgt ggcagacgag cgcgatctaa
    20581 cggattaaat ttaccgctgt tacgcaagtc agcagcaacg atgccgccaa tatcttcagg
    20641 tgccgcacca ggccccgccc actggaaagg aacaacacca ataggacgac cggaatctac
    20701 accgctgtcg atcacaatgc ggacttcagc atgcagaact gatgcccaca gtatgagaaa
    20761 accaaatgct actcgtaatg cctgcttcat catatctccc ttatctggac cgatggccca
    20821 cgataattta gcagaatgtt aacaaactaa aatacacaaa actaccagaa ccccgtggca
    20881 accggtgcct gatgttgacc gtccgaacag tcaacatcgc gattacggtt tgaagtccaa
    20941 tggcgcgttt ttgaacactt catatactgc ctggcttggt ggtttcggga tcttcgcaag
    21001 tttagctgct gccaacgcag cctgacaaag tgcgggatcg ccaccttcag gtttgatatc
    21061 cagtaacata ccatcgggtg ccagttttat gcgcagcgta caggttttgc ctgcatagga
    21121 cgatgcgtca tagaacttac tttcgatagc agatttaatc tgcccggcat agttattgat
    21181 atcggcccct gatgcgccat tgtttttagt attaccactc ccggcaggcg aagcattgtt
    21241 ccctttcgcc cctccccccg ttttcggtgc attcttacca gagcttagct caccgaaaat
    21301 atcatctgcc tctgcggcag cttttgctgc agcggctttt tcagctgctg cttttgccgc
    21361 tgccgctttt ttgtctgcgg cggctttttc tgccgctgct ttcttatcag ctgctgcctt
    21421 atcagcagcc gctttttctg ctgctttttt gtcggctgca gctttctctg ctgccgcttt
    21481 cttatcagct gcagcctttt cagcagccgc tttcttctca gcttctgcct tggctttttc
    21541 agcagcttca gttgctgctt tctttcttgc ttcagctgca gctgcttctg ccgcttccgc
    21601 tttcttcttc agtgccgcag cggctacctc ggcttttttc tgcgcttcgg ctgcggcttt
    21661 ggcggcttct gcttctgctt ttttcttcgc gtctgcagcc gctttcttcg ctgcttcttc
    21721 cgcagcttta gcatctgccg ccgctttcgc tgccgccact tcagcttgct tctgctttaa
    21781 ctcggcctgt tttgcggctt cttcagcctg ctttttctgc tcctgagccg ctaaccgctc
    21841 tttctcaagt tgcttcaggc gttcctgttc agccgcttgt ttctcacgca gttcttcagc
    21901 agcctgctgt tccttcatct tgcgctgttc atcagaacgc ttcgcgcttg attcctggct
    21961 ttgcatgcgt ttgtactgct caactaccgc acctgaatca accatgacag cgtcgatgga
    22021 cgaaccaccg ccgcctccgg ctgaagcttc tatattctca tcgaacgaac tccagatcag
    22081 cgccgcaaat aagatgacat gcagcactgc tgaaataatt atcgcccgct tgagcttgtc
    22141 gttttgttcg gttgcctttg acactctcgg tttccaaaaa ctgttcgcct gttacccgct
    22201 ctctttcaag caagggaaac gcagatgttt agataggctg cgtcattaaa ccaaccgatt
    22261 tcacacccgc actatgtaac aagttcagtg ctttaattat ttcatcgtaa ggcacatctt
    22321 ttgcgccacc gatcagaaag accgttttcg ggttggcctt gaaacggctg gacacttccg
    22381 ccaccacctg ctctggtggt aaacgctcca ggcgatcttt ctcaaccacc acggtgtact
    22441 gaccaatacc agacacttca acaatcactg gcggattatc gttactgctc accgcctgtg
    22501 attcagtagc gtctggcaga tcgacctcca cgctctgggt gatgatgggc gctgtcgcca
    22561 taaagatcag caacagcacc agcagtacgt ccagcaacgg aacaatgttg atttcggact
    22621 tgagatcgcg acgacctcgt ccacgcgctc tggccatggc ttaccccttg ttgctctcgc
    22681 taacggtaaa cgcctggcgg tgcagaatcg cggtaaactc ttccataaag ttgtcgtaat
    22741 tcagttccag tttgtttacg cgctggttga gacggttata agccattacc gccggaattg
    22801 cggcgaacag accaattgcc gtcgcaatca acgcttctgc gataccgggc gcaaccattt
    22861 gcagtgttgc ttgttttacc gccccgaggg cgataaaggc gtgcatgatc ccccagaccg
    22921 taccaaacag accaatatac gggctgatgg agccaaccgt accgaggaac ggaatgtgcg
    22981 tttccagatt ttcaagttca cggttcatgg agatacgcat agcacgcgac gccccttcca
    23041 ctacggcttc cggcgcatgg ctattggcac gatgcaggcg cacaaactct ttgaacccgc
    23101 tgtaaaagat ttgttccgaa ccagtcagat tatcccgttt cccctgactc tcttgataga
    23161 ggcgagagag ttcgattcca gaccagaatt tatcttcaaa cgcttcggct tcacgcgccg
    23221 ctgcgttaag aatacgggtt cgctggataa taatggccca ggatgcgatt gaaaaaccaa
    23281 tcaaaatcaa catgataagt ttaaccagaa ggctagcctt caggaacaaa tcaaggatat
    23341 tcatgtcagt cactgcttaa actccgcgac aatagacttg ggaagcgcac gaggcttcat
    23401 tttgagtggg tcaacgcaaa caaccagaac ctctgcttca ttcagcaaag tattctcggc
    23461 gttgacaata cgttgcgtga aaaccaaaga ggtgccacgc attgatgtta tttcagtctg
    23521 tatttcgagc atatcgtcga gccgcgcagg tgcgtaatat tccaccgtca ttttacgtac
    23581 cacaaaggca acgcgttcag ccatcagcgc ctgctgactg aagtgatgat gacgcagcat
    23641 ctctgtgcgt gctctttcat aaaaagcgac gtaactggcg tggtacacca caccaccggc
    23701 atcggtatct tcatagtaga cgcgaaccgg ccatcgaaac agcgttgtat tcactttaca
    23761 tcccggtaat gcaacaaaag ttagagcttt taaacgctgc tactatacgc gcgcgattga
    23821 ggtttgggaa tgagggcaag ttaagggcgc gtaaaaattt atgggcctgg agcggcccat
    23881 aattttttaa agagaattct gatttaaaag aagaagaaaa tcagcccgac aatgagaaca
    23941 atatcggcaa gcaatgggca aaaaatccct tgccaaagaa ccttctgcgg acgaaagccc
    24001 acgccgtgaa tcacaccagc acagacggcc cacatcagca ataaaccatg ccagatttcc
    24061 agttcactgg tcttcgcggc aaaacgagat gggtcccaaa acatacatcc tgctaacaga
    24121 agcgccatca cgaaggaaag cgcccgcagg gggcgcttgt ccattaccgc atacaaggtc
    24181 gcgataatct tactcatcag atgtcttctt gaccggcttt gcctgattcg acgtgttcaa
    24241 gcgccagcgc ggtgattacc ccaaacgaac aggcaagaag cgttcccaga atccatgcga
    24301 aataccacat ttttagctcc ttacttagta cagagagtgg gtgttacgtt caatatcttc
    24361 tttggtgata cgaccgaaca ttttccagta acaccaggcg gtgtagagca gaatgatcgg
    24421 taccagaacc accgcaaccc aggtcatgac gttaagcgtc agctggctgg aagttgcatc
    24481 ccacattgtc agacttgcgt tcatcatggt gctggacggc atcacaaacg ggaacattgc
    24541 gataccggct gtcaggatga tgcaggccag cgtcagggag gagaacacaa acgcccacgc
    24601 ggctttatcc atacgtgcag tcaggatggt cagcagcagc agaaccacac ccagtgccgg
    24661 aatagcccac agaattggcg tgttgttgaa gttaaccagc catgcgccag cttcacgaac
    24721 cacttcttta ttcagtgggt tagaggctgc gtaatggtcc attgtcgatt tcacgacata
    24781 accatcgata ccgtacatca cccatacgcc agccagtgcg aaacagacca gtgtcaccag
    24841 cgcagccacc tgagccgttg cacgggtacg caggtgcagt tcgcccacgg tacgcatttg
    24901 cagataggtt gcgccctgag taatgatcat ccctacgctc accacgcctg ccagcaggcc
    24961 gaacgggtta agcaactgga agaagttacc ggtgtagtac agacgcagat attcatcaac
    25021 gttgaacggt acgccctgca acaggttacc gaacgctaca ccaattacca gcggcggaac
    25081 gaagctacca atgaagatgc cccagtccca catgttacgc cagcgggttt cttcaatctt
    25141 ggagcggtag tcaaaaccga ccggacggaa gaacaaagac gccagcacga ggatcatcgc
    25201 cacatagaag ccggagaacg cagcggcata gaccatcggc caggcagcaa agagtgcgcc
    25261 gcccgcggtg atcagccaaa cctggttacc gtcccagtgt ggtgcaatgg agttaatcat
    25321 aattcgacgc tcggtgtcgt tacgaccgag gaaacgggtg agcatgccca cccccatgtc
    25381 gaaaccgtca gtgactgcaa aaccaatcag cagaacgcca accagcagcc accagataaa
    25441 acgcaatact tcataatcga tcatttgacg actcctgtct tagcgtgccg gctgagtagt
    25501 cgtggaagac tgctcaaagt gatagcgacc ggttttcagg ctgcttgggc cgaggcgtgc
    25561 aaacttgaac attaagaaca attctgccac caggaacagg gtatacaggc cgcaaatcag
    25621 caccattgag aagatgagat cgcctgcggt cagtgacgaa ttcgccacag ctgtcggcag
    25681 cacttcaccg atagcccacg gttggcggcc atattcagcc acgaaccagc ccgcttctac
    25741 agcaatccac ggcagcggaa taccgtacag cgcggcgcgc agaagccatt ttttctcgcc
    25801 aatgcggttg cggatgacac tccagaaaga gagcgcgatg attgccagca gcaggaagcc
    25861 acacgccacc atgatacgga acgcaaagta cagcggcgct acacgcggga tggagtcttt
    25921 ggttgcctgt tgaatctgcg cttcagtcgc atcagccacg tttggcgtat agcgtttcag
    25981 cagcagaccg taaccgaggt ctttcttcat gctattgaac tggtcacgaa ccgcctggtc
    26041 ggtagaacca gaacgcagtt gttcgagcag agagtacgcc ttcatcccgt tacgaatgcg
    26101 ttcttcatgc tgcaccatca gctctttcag gccgataacc ggggtatcca cggaacgcgt
    26161 tgcaatgatg cccagtgcgt aagggatctg aatcgcaaat ttgttcgtct cctcttcctg
    26221 atcaggaatg ccgaacaaag taaaggcagc aggcgcaggt tgcgtttccc actcggcttc
    26281 aatagcagcc agtttggttt tctgcacgtc gcccatttcg tagccggatt catcacccag
    26341 aacaataaca gacagaacag cagccatacc gaagctggca gcgatagcaa aggagcgttt
    26401 agcgaaggcg aagtcacgac ctttcagcat ataccatgcg ctgataccga ggatgaacat
    26461 cgcgccagtc acataaccag acgctacagt gtgaacgaat ttcacctgag caaccgggtt
    26521 aagcaccagc tcggagaagc tcaccatctc catacgcata gtttcaaagt tgaaatcgga
    26581 cgcgattggg ttttgcatcc agccgttcgc aaccagaatc cacagtgcgg acaggtttga
    26641 accgagcgcc accagccagg tgacacacat atgctgaact ttacccagac gatcccaacc
    26701 gaagaagaac agacctacaa aggtggattc gaggaagaag gccatcagac cttcgattgc
    26761 cagcggcgca ccgaagatat cccctacata gtgggaatag taagaccagt tagtcccgaa
    26821 ctggaactcc atggtcagac cggtagccac acccagagcg aagttgatac caaacaactt
    26881 gccccagaac ttggtcatat ctttataaat ctgtttgccg gagaggacgt agaccgtttc
    26941 cataatggcc aggaggaacg ccataccgag cgtcagtggc acaaaaagga agtggtacat
    27001 cgcggtcaag gcaaactgta agcgcgacag ttcgactata tctaacatca tgactccttg
    27061 ctcatcgcat gaagactccg agagtgaacc ccgttagaaa gggtcacacg catgccccaa
    27121 tagaaatttg tttgaacact ttcatcatac catgtgaatg cacacaaaaa cggcgtaaag
    27181 aaaggttgca aacatgttaa taaaaactca aattgatccc acgtatatat tacgccgcaa
    27241 aatccttaca ataaacaggt ttttgttgaa gcaaatttgc atttttcgac ggtgatcaat
    27301 ttatagctaa attgccgcct ttcagccaat ttgatcgaga acaatttatc tctttttgat
    27361 gcccatttcc aagacttata cattgataaa tatcaatttc tacaaattat gttaatcatg
    27421 tttcaacaag gtaactttag tgatatttat gtttaaaatg tgtgaattat aagactgaaa
    27481 ttagtttcgg ttggcaatta aagataaaaa gagacgaagc aaaaacagca cggttgacct
    27541 taattctttt tttggtcact ggctaatacg acgcagagtt tcaggattat taataaaggc
    27601 ataaatctcc cccccgccga tattaacatt taattaactt ataattatat tataattaac
    27661 tcacccgtgg ttattatttt gttaaaaata aagccacata tacgtagctt gagagtgaag
    27721 ataaacacaa agttacctga tgcgctacgc ttgccagtgt ctacatcatc tctgcaatat
    27781 attgaatttg cctgcttttg tagaccgaat aaagcgttca tgccgtatcc ggcatgaaca
    27841 aagcgccctt tgtcaacaat ctggccgcgc atatgtggct tcggtttgat tgctattcag
    27901 gcaagccggt aactgaacgt ccgtgactgg ccgggtaaaa aaggccccga tagatttgaa
    27961 ccttgatttt cctcggtggt aatgtgttca tccatcatcg tttctgagca agaaatcacc
    28021 tcgcgactga aagcaacagt cgcatcacag gttgctgatt cagccggatt aaacagccgt
    28081 aaaatcactt cttgtcggtc ttcagctttc ttaagtgcgc ttatcaggca tcccactggg
    28141 ggcattttca acaaactata actttccggc acgttgaatc cggctttgtt gagcttcatc
    28201 gcatcccatg ggattttgtt gtaacactgt actggagtca gccatgctcg cgcctgctga
    28261 gctacaccag cggcggttgg cgtaccggta taactcaata aacttaggcg acaagaaagc
    28321 agaccacgta gttgtgagtc cgggactggc attttaattc ccgaaggccg cctaggcctt
    28381 aaaagcagat cttctttccc cagtaagccc acgccgcgca gcaacgtaat ggcaaaggtt
    28441 ttcttctctt caccgatgac ttcaaattca cgtagccctt cgctaaagac agccatgccg
    28501 ttacgccctt cctgtaaggc tgcatagttg agcatattcc ataccggaac cggcgcttct
    28561 ttccagcctt cttgctgcca gttgttcatt gcactgtcgt tcacggggcg cgttagcgaa
    28621 ccaaactggg tatctgccag aacactatcg gtgttaaaag atgtagggat caggacgcga
    28681 aggcgatgat cgtcagcctg gttatcaagg ttgatatcca catcaatgcg cctgttgtta
    28741 tgactaagag tgacaaccaa ctcgacatct actctgccag tggattgtct tgcgctgcgt
    28801 tgttgcagat tacgcggcac cgccatttca tagcggataa cagccctgct ctgccaggcc
    28861 tcatgaataa tctcgcattg cggtttcgca ttcgccgaag taattaccca ctcttctttt
    28921 gcgggtgaat agtcatattc atcaccatca tcagagcttt cttcaatttg caacacccgg
    28981 tcatagcgca caccgctatc tttatctacc agttgtagcg ttccatcctc attgagcgca
    29041 atttgccaga aagcattttc cagtatccct tcagcgtcac tttttgccgc aatcacgttg
    29101 ccaggctgat tcgcttcgat ataaagcgtg cgatagccca tagaagggac aatctggttg
    29161 atctgtatat caaactccat aaagggatcg taattaccgt aatgaactat ttgccgatcg
    29221 attaggcctg gatcgatctc acgggcatgg cgaataaaat acggtacagg ctgaccgcga
    29281 tcgtctcgta gattaaactg gctggcgcgc agccgcacag tggtgttgat cacttcttcc
    29341 cgcggccagg gcatcaggtt aaacaggacg agtttgtcgg cgtcgctctg cggcatgttg
    29401 tcggcgattt tacgcatata gaaacgcagc agattatccg ccatgtcttc agccagttcg
    29461 aagcgggcga cgatttcgcg atgaactttg tcactacagc agcagccgat gctgtcgtgg
    29521 gcatgatttt ttaagatctc tttccacatt ttctccagca agccgtggtg gtatccaaaa
    29581 cccaacgtcc tggccagcgt tgccagcggt tccagcagat taacaatctt attttcaata
    29641 cgtgcgtggg caactttgat atccatgcgc gtggaaccga tggtgcgatg cacgcgcata
    29701 tatttgccat caataaattc ccctttcagg gttgccagat tatctcgctg cgcttcgatc
    29761 ttctcaaata cctcttcaaa gcggctcatc acaaactttc gttgaggata aatttcgcgc
    29821 agtttctcca tcacttcgaa gatattttgc tgcaatggca tctgatcatg cccattcggc
    29881 agcaaaatct ctttggttac agacgctttt tccagcacgt caaaataact gtcgaggcgt
    29941 ttgcgtaatc cgttttcgtc cgcaggtaag tacttaccga tggcataacc caacggcatc
    30001 acctgcgccg tcacttcgct accgtcactg ctttgccaca aaaactcggt tttatccgta
    30061 ccgtggcgct cagaacatcc acgccagaac atggtgcagg taatgccaaa tccattgtag
    30121 atatgcggca gttgcccgga catgccaaag gaatcgggta aataacctat tttcatcggc
    30181 tcgccaaacg cgaggcagtc acgcattccg tacatcagat tacggacgat ggattccgca
    30241 gaaacaatcg tggtatcggt ctgggtatac taggggccga taatcaactt gccggcttct
    30301 accagtttct tcacgcggtc tttgttttcc ggtttcaccg cgaaataatc ttcgaggatc
    30361 gccgtttgcc cgtcgagtac gtaatatttg tattcgttgt cctgttccag tcggcacagg
    30421 atctcttcca tattattgac cagcagaatg cgtgactctt cggtggtgaa ataccactcc
    30481 cgatcccagt gcatatgcgg ggtgatgtga acgcgagata ccgctttcat cgtcgtttcc
    30541 tttttgttta tggcattacg ccatcagtca gatagttgcc atgcttaacc gcgtgacgcc
    30601 gccagatcag gagaattgca gtcgagattg cagcccccac caatgccgca ccaaaccagc
    30661 caattgccgc cataacaccg cccgcgccat tatcatgaag taaaaagagc gagaaaatgc
    30721 cagcaccggg tgtcgaaagg ccgatattca tcgcaccgac aatagcgccc gttaccatag
    30781 agcccagcac aaacgaacca ataacccgca gcggatcttc aatcgccatc gggatcgccc
    30841 cttcggtaat acctgccagc cctaacagcc aggtggattt cccggtctca atttcaaact
    30901 ctttaaacag gcgcggtgcg agcatcgtgg aagcggttac ggtaaatgcc gaaaccattt
    30961 tgacggaggc gaaaatggca tacgggccgt aaacgccgtt cgccattgcg cccaggcaga
    31021 atgcataagc ggctttattc actggccctc caaggtcaaa ggaacacata aaaccgagaa
    31081 tggcacccag caacagcgcg ttacttcctg acagaccgtt cagccaggcg gtaagcgagt
    31141 tattgatcca ggcgacaggt tcccccacca caaacagcat cagactgccc gctcccaatg
    31201 taccgagcac cgggtagaga taaaaagtca ggaagccatt gaatttactg ctaagacgca
    31261 agtgattttt catccagcgt atcaagtaac cggctatcaa tccgccaacg accgcgccga
    31321 gaaacccgga gccgatcatg ttggcggcaa gtccagccgc aaagcctggc gctaacgccg
    31381 gtttatctgc cagcgaatag gcggtatagg ctgccagcac cggtaccatc aaaattccga
    31441 gcagcccgcc gcccagtttg cggtacatcc acagccagga gttttcttca ttaaacagat
    31501 cttgtagccc gaagatttgc gacagtaata ccgcgaccgc cagcaccgtc ccccccgcga
    31561 caatcaacgg tacggcaaaa gagatcccgc tcaacagtgc ctgtttcagc tccgttttga
    31621 cgcttttcac cggttgcgta tcttgttgta aggtacgcgt ctcatcgcca cgctcgaggg
    31681 taagcgattg ttggatcaac gcttctgcat ggcgaatcgg ctcggcaaca ggcacagaaa
    31741 gcgcaggaat gccgttaaaa cgctcactct ccttgatggc gacttcagcc gcaaaaatac
    31801 aggcagttgc actattgagt tgatccgccg ttaaacgccc ttcaataccg ttagcacctt
    31861 gcttttcaac gtagacattc acgccgagtt tgcgtccggc tttttccaga tactccgcag
    31921 ccatgtaggt gtgagcaata cccgccgggc aggccgttac gcaaacgata gttggcgcgt
    31981 tggaaaaaga ggcagaaggt tgcgtgcctc ccttgtcatc cagcgccgag agcaattcat
    32041 caggcgtcgt cgccgactgt atacgcgccc gaatctcatc atccgccagg cgcgtggtca
    32101 gcgctgtcag cagttgcatg tgcgtagtac ccgcttcatt gaggggaatc gccagcaaca
    32161 ccactaaatc aaccgcttcc gggccatcca cgccttccca ctgaagcggc tcgctgagtg
    32221 tggcgaccgc aaacgccgct tcttttaccg cagcagtttt gccatgcggc acagccaacc
    32281 cttcacttaa ggcggtcggg ccaaggcttt cacggcgata cacttctttc agaaattgct
    32341 cagtactgga aattttcccc agagcagcaa gacgttgagt caacgcgtga atggcctctt
    32401 cacggctggt aaagcgcgca ttcagacaca acgcatcgcg gtgggtcaga gtcgtcaggt
    32461 tcatcatgat cctcacttga tcgtttttat agtcgcgcca gtgtgcccga caaaacaata
    32521 ccattgcgta atcaatttca cttgattgaa attatttgca ttttatttgt attgtttaca
    32581 ggatgaattt atcgggcata atggctaaat tcgtattaat gagatgctaa ataatgggac
    32641 acaaaccctt ataccgacag attgctgatc gcattcgtga gcaaattgcg cgtggcgagc
    32701 ttaaacctgg cgatgcgtta cctaccgaat cggcattaca aacagagttt ggcgtcagcc
    32761 gggttacggt gcgtcaggct ttgcgccagt tagtcgagca acagatcctc gaaagcattc
    32821 agggcagcgg gacttacgtc aaagaagagc gggtcaatta cgatattttt cagttaacca
    32881 gttttgatga aaaactgtcg gaccgtcacg tcgatacaca cagtgaagtt ctgatatttg
    32941 aagtgattcc ggctgacgat tttcttcagc aacagctaca aatcaccgtg caggatcgtg
    33001 tgtggcatgt gaagcgcgtg cgctatcgca agcaaaagcc aatggcgctg gaagaaacct
    33061 ggatgccgct tgcgttgttc ccggatctca cctggcaggt catggaaaat tcgaaatatc
    33121 actttattga agaagtgaag aagatggtta tcgatcgtag cgaacaggaa atcattcctc
    33181 tgatgccaac cgaagagatg agccgtctgc ttaatatcag ccagacaaag ccgattcttg
    33241 aaaaagtatc gcgtggatat ttagttgatg gtcgggtgtt tgaatatagc cgcaacgctt
    33301 ttaataccga tgactataaa tttaccctga tagcgcagcg aaaatcatcg cgataagcac
    33361 aaaaaggcca tcatacgatg gccaaccatg tcgaaaccga cagcccttcg ccattcctgt
    33421 agagggaagc ggcgagggct atttcttatt acagatattt atttcagaac agttttcagt
    33481 gcttcaccga tatccgccag gctgcgaacg gttttcacgc ctgcggcttc cagagcagcg
    33541 aatttctcat ccgcagtccc tttcccaccg gcaatgatcg cacccgcgtg gcccatacgt
    33601 ttgcctttcg gcgcagtcac accagcgatg taacccacaa ctggcttggt aacatgctct
    33661 ttgatgtacg ctgccgcttc ttcttcagcg ctaccgccga tctcaccgat catcacgatc
    33721 gcttcggtct gcggatcttt ttcgaacatt tcgagaatgt cgataaagtt agagccaggg
    33781 atcgggtcac cgccgatacc gacacaggtc gactgaccga aaccgtaatc cgtggtctgt
    33841 ttaaccgctt cataggtcag tgtaccggaa cgggaaacga tacccacttt acccggtttg
    33901 tgaatgtgac ccggctggat accaattttg cattcacccg gggtgataac gcctgggcag
    33961 ttcgggccga tcatacgaac gcctgcttcg tccagtttca ctttcacggt cagcatatcc
    34021 agcgtcggga tgccttcagt gatggtgata atcagtttga tgcctgcatc gatagcttcc
    34081 agaatggagt ctttgcagaa cggtgctggt acgtaaataa cagacgcgga agcgccagta
    34141 gcagcaacag cttcacgcac ggtgttgaac accggcaggc cgaggtgggt agtgccgcct
    34201 ttacctggag ttacgccacc aaccatttta gtgccgtatg caatggcctg ttctgagtgg
    34261 aaagtcccct ggctaccggt aaagccctgg cagataacct tggtgttttt atcaattaaa
    34321 atggacatta tttcccctcc actgcggcaa caacctgctg agctgcatcc gtcagacctt
    34381 ttgctgcaat aatattcagg ccgctgtcag ccagtttctt cgcgccgagt tcggcgttgt
    34441 taccttccag acgtaccacg accggtacgt taacacccac ttccgctacc gcgccgatga
    34501 taccgtcagc gatcaggtcg caacgaacga taccgccgaa gatgttaacc agaacggctt
    34561 tcactttgtc gtcagagagg atgattttga acgcttcggt tacacgttct ttggttgcgc
    34621 cgccgccaac gtccaggaag ttagccggtt cgccgccgtg cagtttaact atgtccatcg
    34681 tacccatcgc cagacctgcg ccgttaacca tacaaccgat gttaccgtcc agcgcaacgt
    34741 agttcagttc ccactgtgca gcctgtgctt cacgcggatc ttcctgcgac tggtcacgca
    34801 tttcgcgcag atcaggctgg cggaacaggg cgttgccatc agcgcccagt ttgccgtcga
    34861 ggcaaatcag atcgccctgt ttggtgatga ccagcgggtt gatttcgatc agcgccaggt
    34921 cgcgctccag gaaaatggtc gccaggccca tgaagatttt ggtgaactgc tgaaccagtt
    34981 taccttccag acccagtttg aacgccagct cgcgtccctg atacggcatc gggccagtca
    35041 gcggatcaag cgcaacttta tggatcaggt gcggagtttc ttccgccact ttttcgattt
    35101 ccacgccgcc ttcggtggag gccataaaga ccacgcgacg ggaactacgg tctacaacgg
    35161 caccgagata cagctcttta gcgatatcgg tcgccgcttc aaccagaatc tggttaaccg
    35221 gttggccatt ggcatctgtt tgatacgtta ccagacgctt gcccagccag ttttctgcaa
    35281 aagcacggat gtcttctttg ctgtttacaa ctttcacacc gcccgcttta ccgcggccac
    35341 cagcgtgaac ctgacatttc actacccacg gaccggcacc gatttttgaa gcggcttctt
    35401 ctgcttcgcg cggagtagta caggcataac ccaccggtgc tggtaagcca tagcgggcaa
    35461 acagttgttt tgcctgatat tcatgtaagt tcatgtgttc tgtccatcct tcagtaatcg
    35521 ttatctttta aaccgtagac cgacacccta tccacgtcgg tctacagtgc aggtgaagct
    35581 taaactacta cacgtccagc agcagacgcg tcggctcttc cagtaactct ttgatcgtta
    35641 ccaggaagcc cacggattcg cgaccatcga tcagacggtg atcgtaggac agcgccaggt
    35701 acatcatcgg caggatctca acctgaccat tcaccgccat cggacgatct ttgatagcgt
    35761 gcatacccag aattgcgctc tgcggcgggt tgatgatcgg cgtagacatc agggaaccga
    35821 acacaccacc gttggtgatg gtgaagttac caccggtcag atcttcaacc gtcagcttgc
    35881 catcacggcc tttgactgcc agctctttga ttttcttctc gatgtctgcc atgccgaggg
    35941 tatcgacatc acgcagaacc ggcgtcacca ggccgcgcgg cgtagaaacc gccatgctga
    36001 cgtcgaaata gttgtggtaa accacgtcat cgccgtcgat agacgcgttc acttccgggt
    36061 aacgtttcag ggcttcaacc accgctttca cgtagaagga cataaagccc agacggatgc
    36121 cgtggcgttt ttcaaacgct tcaccgtact gcttacgcag atccataatc ggcttcatgt
    36181 tgacttcgtt gaacgtggtc agcatggcgg tggagttttt cgcttccagc agacgctctg
    36241 ccacacgctt acgcaggcga gtcatcggga cacgtttttc actacgtgca gccagagccg
    36301 gttgcgccgc cggagcagcc gctgccggag cagactcttt cgccggggct ttcgccagat
    36361 gtttttccac atcttcacga gtcagacgac cacccacacc ggtgccttta atggcgctgg
    36421 cgtcgagatt gtgttcagcc agcaggcgac ggatcgccgg gcttaacgca tcgttgtttt
    36481 gctcttccag agacgcctgc tggcgttgcg ccggagtgga cgctttctct tcagatttgg
    36541 cgctggtttc tttaccggcg ctgttgcctt cacgcaggcg accaaggatc tgacgagacg
    36601 ttaccgttgt accttcatct tccagaaccg catccagaat gccgtctgct gatgccggta
    36661 cttccagtac cactttgtca gtttcgattt ctaccagcac ttcatcacgt acgactgcgt
    36721 cgccgggttt tttatgccag gttgcgacgg tggcatcggc tacggattca ggcaggtcag
    36781 ggaccagaat atctacgcta ctcattgtgt atcctttatt tattcgacgt tcagcgcgtc
    36841 attaaccaga tcttgttgct gtttctggtg aacggacata taccctaccg ccggagaggc
    36901 ggaggccggg cggcctgcat aacgcagaga agccccaaac ggaatcactt cacggaaatg
    36961 atgctggctg cagtaccatg cgccctggtt gagcggctct tcctggcacc agacaaaatc
    37021 cttgacgtga gcaaactgct gcaacacttc ctgcatcgct ttatgcggga acgggtagag
    37081 ttgctcgata cgcacaatgg cgacatcgtg ttgattgttc ttacgacgct gttccagcag
    37141 gtcgtaataa accttaccag aacacatcac tacgcgcttc acgcccttcg gatcaagctc
    37201 gtcgatttca ccgatggctg gcaggaaggt gccgttcgcc agttcttcga ggctggaaac
    37261 cgccagcgga tgacgcagca gggatttcgg cgacatcacg accagcggac gacgcatccc
    37321 gcgcagcgcc tgacgacgca gcatgtggta aacctgtgcc ggggtagacg gtacgcaaac
    37381 ctgcatgttt tgctcagcac aaagttgcag ataacgttcc agacgcgcgg aggagtgctc
    37441 cggcccctgc ccttcgtaac cgtgcggcag caacatcacc agaccacaca tccggcccca
    37501 tttctgctcg ccagaggaga tgaactggtc gataaccacc tgcgcaccgt tggcgaagtc
    37561 accgaactgc gcttcccaga tggtcagggt gcgtggttct gcggtggcat aaccatattc
    37621 aaacgccagc acggcttctt ctgacagtac ggagtcccag acacggaacg cgccctgacc
    37681 gttatggatg tgttgcagcg gcgtgtaagt ggaaccgtta gactggttgt ggatcaccgc
    37741 gtggcggtgg aagaaggtac cgcgaccgga gtcttcaccc gacaggcgaa ccggaatgcc
    37801 ttcatcaacc agcgtggcgt aagcgaggtt ttccgcaccg ccccagtcga acagtttctc
    37861 accggctgcc atcgcctggc gatcgccata aatcttggca acgcgagact gcatttcaac
    37921 tgcttctggt accgtactga tacgtttagc cagttcctgc aggcgcttca tctcaacttt
    37981 gttcgggtac tcttcgtccc attcgtggtt gaggtacggc gaccaggtga aagagtgcat
    38041 gttcatcgga cgccactctg ccactacgca atcgccagca tccagcgcat cgcggtacag
    38101 gttaaccatc tcggtggcat cttccagcgt cgccactttt tcctgctcca gcttgtcagc
    38161 gtagattttg cgcggtgtcg gatgtttttt gattttctga tacatcagtg gctgggttgc
    38221 gctcggctcg tcggcttcgt tgtggccgtg acggcggtag cataccaggt cgatgaagac
    38281 atcacgttta aaggtgttac ggaaatcgag cgccagacgg gtcacaaagg caacggcttc
    38341 cggatcatcc gcgttaacgt ggaaaatcgg tgcctgaacc atcttaccga tatcagtaca
    38401 gtacggtgta gaacgggcat ccagcgggtt agaggtggtg aaaccaacct ggttgttgat
    38461 aacgatacgt accgtaccgc caacttcata accacgcgct ttcgacatgt tcagagtttc
    38521 ctgaaccacg ccctgcccgg tcactgcggc gtcaccgtgg atggtgattg gcagcacttt
    38581 gttgctgttc ggctcatcaa gtctgtccag acgggcacga acagagccga taacgaccgg
    38641 gcttacaatc tcaaggtgag acgggttaaa cgccagcgcc aggtgcacca ggccgccatc
    38701 ggtctggaag tcagacgaga agcccatgtg gtatttcacg tcgcctgtgc cgaggtgttc
    38761 tttatgttta ccggcgaact cgtcgaacaa gtcttgcggt tttttaccca gcacgttcac
    38821 cagcacgttc agacgaccac ggtgcgccat cccgagaacc acttcgcggg tgccgctgtt
    38881 gccagcgtgg cggatcatct ctttaagcat cgggattaac gcgtcaccgc cttccagcga
    38941 gaagcgtttt gcgccaggga attttgcgcc gaggtaacgc tcaaggcctt cagcggcggt
    39001 cagttcgctt aagaagcgtt ttttctcttc gctattgaaa gtcgcgcgac cagactcgat
    39061 acgctgttgg atccagcgtt tttcttcggt gctggtgatg tgcatatact cggcaccaat
    39121 cgggccgcag taggtttgct tgagggcgga aataagctcg ccgagtttca tggtttcttt
    39181 gccgctggca aatgaaccga cgttgaaggt ttcctggaag tctgcttcgg tcagatcgtg
    39241 gaaagacgga tccagatcgg ccactttatc ttgctgccac agtcccagcg gatctagatt
    39301 cgcatgctgg tgaccacgga agcggtatgc gttaatgagc tgcaggactt taacctgctt
    39361 cacattggtg tcagggtcgg agatcgttga agagtaacgt gaagcgtctt tcgccaggcg
    39421 gcggaaatat tcacgcgttt gagagtggaa ttgatccggt ttgactcccg taccaggtaa
    39481 ctgctggaac gtcgaacgcc agttagcgtc aaccgagtca ggatcggtta agaagtcttc
    39541 atagagctgt tctatccagc tctggtttgc gccagagagg taagaagagt ccaaccaggc
    39601 tttcaaagcg ctgttctgca tcgtgatccc ttaagcatct tttttatgct tacttcgccg
    39661 tggatactac cacgcacagt gatgtgcgtg ccggggttca cttgcgagct cttgtctacg
    39721 tagtggctcg cgaaggaacc tttaaaaact gtcttagtgt caggggcagt ttttaaagat
    39781 ttcctgcatt tctttgttac gcctgatgcg ctgcgcttat caggcctgcg ggtaatgact
    39841 tgtaggccgg ataaggcgtt cacgccgcat ccggcactgg ttgcctgatg caacgcttgc
    39901 gcgtcttatc aggcctacag tttacgcatt acgttgcaac aacatcgact tgatatggcc
    39961 gatggcgcgc gtcgggttca gccccttcgg acatacactg acgcagttca tgatgctgtg
    40021 acagcggaat acgctgaatg catcgctcaa accgtcgagg cggctgtcag tctcggtatc
    40081 acggctatcg atcaggaaac gatatgccgc taacaagcct gccgggccga taaacttatc
    40141 gggattccac cagaaagacg gacaagaggt tgaacaacat gcgcagagaa tacattcata
    40201 cagcccgtcg agtttttcgc gctgctctgg catctgtaaa tgctcgcgag ctggcggatt
    40261 ttgtccatta ttcaacaggt aaggcttaat tttctcatat tgcgcataga attgtcccat
    40321 gtctaccacc aaatcgcgga tcaccggtaa acctggcagc gggcgaatca caatcttctt
    40381 gcccggctgg ttgagtgccg aaatcggggt aatacaggcc agaccattct tgccgttcat
    40441 gttcagaccg tcggaaccgc acacaccttc acggcaggag cggcggaacg acaggctggg
    40501 atctttctct ttcagctgga taagcgcatc cagcagcatc atgtcgcgac cttcttccgc
    40561 ttccagggtg taatcctgca tacgcggagc atcatcaaca tccgggttat agcgataaat
    40621 tgaaaactcg agtctcattt tcctgtctcc gcattagtaa gtacgaatct tcggcgggaa
    40681 tgccgggcgc agtttcggtt ccatgttgac gcttcggcgc gtcatggatt ccgactcagg
    40741 cagatacagg gagtggcaca gccagttttc atcatcacga tccgggaagt cgaagcggct
    40801 atgtgcgcca cggctttcgg tacggaagtt ggcagaaaca gccgttgcat acgccgtttc
    40861 catcaggtta tccagttcca ggcactcaac gcgctgggtg ttgaactcgc tggaagtgtc
    40921 atccagacgg gcatttttca gacgctcgcg gatcactttc aactgctcaa gccctttcgc
    40981 catcgcatca ccttcacgga agaccgagaa gttatgctgc atacattctt gcagcgcttt
    41041 acggatcgcc accggatctt caccgttacg attattgttc caccggttca ggcgatccag
    41101 agacgcttca acatcagact cgctggcatc gtgcagtgcg ccctgctcgg cgatagactc
    41161 ttgcagatgc agacctgccg cgcgaccaaa gaccaccagg tccagcagcg agttgccgcc
    41221 cagacggtta gcgccgtgta ccgatacaca agcgatttca ccaacggcaa acagtcccgg
    41281 aacaaccaca tcttcgcctt tctcattcac agtcagcgcc tgaccggtaa ctttggtcgg
    41341 aataccgccc atcatgtagt gacaggttgg gataaccgga atcggctctt tcaccggatc
    41401 gacgtgagcg aaggtacggg aaagctccag gatacccggc agacgggatt cgagaacttc
    41461 tttacccagg tgatcgagtt tcagtttcgc gtgtggcccc cacggaccat cacagccgcg
    41521 accttcacgg atttcaatca tgatggaacg cgccaccaca tcacggcccg ccaggtcttt
    41581 ggcgttcggc gcataacgct ccataaagcg ttcgccatgt ttgttcagca gataaccgcc
    41641 ttcaccacgg caaccttcgg tgaccagtac gcccgcaccg gcaatgccgg tcgggtggaa
    41701 ttgccacatt tccatatcct gcaccggtac gcctgcgcgg atagccatgc cgacaccgtc
    41761 gccagtgtta atgtgggcgt tagtggtgga ctgataaata cgccctgctc cgccagtcgc
    41821 cagcacggta gcgcgggctt tgaaataaac cacttcaccg gtttcgatgc acagtgcggt
    41881 acaacccacc accgcgccat cctggttttt caccagatcc agcgcatacc actcggagaa
    41941 aatggtggtg tggtttttca ggttctgctg ataaagcgtg tgcaacagtg cgtgaccggt
    42001 acggtcagca gccgccgcag tgcgtgccgc ctgctcgccg ccgaagtttt tcgactgacc
    42061 gccaaacgga cgttgataga tacgaccatc atcgagacgc gagaacggca ggcccatgtg
    42121 ttcgagttcc agaatcgctt ccggcccggt tttacacata tattcaatcg cgtcctggtc
    42181 accgatatag tccgaccctt ttacggtgtc gtacatatgc cattcccagt tatcttcatg
    42241 ggtattaccc agcgcaacgg taatgccgcc ttgcgcagaa acggtatggg aacgggtcgg
    42301 gaagacttta gagagcaggg cacaggtctg gccgctctgg gaaatttgca gcgccgcgcg
    42361 catacctgcg ccaccggcac caatcacaac tgcatcaaat tctctgactg gcaatttcat
    42421 cacacacccc acaccacaac gaatccataa atcacgtaaa ccaccagtgc aacgacaatc
    42481 accagttgca gcatcaggcg caaggccagc ggtttaacgt agtcggtcaa cacctgccac
    42541 atgccgatcc aggcatgaat caagatagaa aacagcgcca gcagggtgaa cactttggtg
    42601 aacgcagagg cgaagaaacc gatccagact tcatatgtca gttcgccact ggtggcgaaa
    42661 aaaccgacca tataaatgat gtagagcgtc aggacgatag cggtagcgcg aacgaggatg
    42721 aaatcatgta cgccattgcg tcctaatgcg gaggcgttgc ttaccatacg aggactcctg
    42781 cgagaagtga aagcacgaca gtaataacaa aggagatttt ggcggagcgt ttacccgctt
    42841 cgaatgtttc ttccagatag ccaaaatcca tcatcatgtg gcgaatacct acgacgacgt
    42901 gatacgccag agcggtaagg atgccccaca tgataaattt gacgaagaag ctgcccataa
    42961 tcgcggaagc ttgctcgaaa ccttcagggg aagagaggct ggtacccaga agccactgca
    43021 ggatgcccac tgcaacaaag gtgatcacac cggaaacgcg atggagaatg gacgctatcg
    43081 ccgtgatggg gaaccggatg gtctgtaggt ccagattaac aggtctttgt tttttcacat
    43141 ttcttatcat gaataacgcc cacatgctgt tcttattatt ccctggggac tacgggcaca
    43201 gaggttaact ttctgttacc tggagacgtc gggatttcct tcctccggtc tgcttgcggg
    43261 tcagacagcg tcctttccat aactgcgcgt catgcaaaac actgcttcca gatgcgaaaa
    43321 cgacacgtta caacgctggg tggctcggga ttgcagggtg ttccggagac ctggcggcag
    43381 tataggctgt tcacaaaatc attacaatta acctacatat agtttgtcgg gttttatcct
    43441 gaacagtgat ccaggtcacg ataacaacat ttatttaatt tttaatcatc taatttgaca
    43501 atcattcaac aaagttgtta caaacattac caggaaaagc atataatgcg taaaagttat
    43561 gaagtcggta tttcacctaa gattaactta tgtaacagtg tggaagtatt gaccaattca
    43621 ttcgggacag ttattagtgg tagacaagtt taataattcg gattgctaag tacttgattc
    43681 gccatttatt cgtcatcaat ggattcttta cctgcaagcg cccagagctc tgtacccagg
    43741 ttttcccctc tttcacagag cggcgagcca aataacaaac gggtaaagcc aggttgatgt
    43801 gcgaaggcaa atttaagttc cggcagtctt acgcaataag gcgctaagga gaccttaaat
    43861 ggctgataca aaagcaaaac tcaacctcaa cggggacaca gctgttgaac tggatgtgct
    43921 gaaaggcacg ctgggtcaag atgttattga tatccgtact ctcggttcaa aaggtgtgtt
    43981 cacctttgac ccaggcttca cttcaaccgc atcctgcgaa tctaaaatta cttttattga
    44041 tggtgatgaa ggtattttgc tgcaccgcgg tttcccgatc gatcagctgg cgaccgattc
    44101 taactaccta gaagtttgtt acatcctgct gaatggtgaa aaaccgactc aggaacagta
    44161 tgacgaattt aaaactacgg tgacccgtca taccatgatc cacgagcaga ttacccgtct
    44221 gttccacgct ttccgtcgcg actcacatcc aatggcagtc atgtgtggta ttaccggcgc
    44281 gctggcggcg ttctatcacg actcgctgga tgttaacaat cctcgtcatc gtgaaattgc
    44341 cgcgttccgc ctgctgtcga aaatgccgac catggccgcg atgtgttaca agtattccat
    44401 tggtcagcca tttgtttatc cgcgcaacga tctctcctat gccggtaact tcctgaatat
    44461 gatgttctcc acgccgtgcg aaccgtatga agttaatccg attctggaac gtgctatgga
    44521 ccgtattctg atcctgcacg ctgaccatga acagaacgcc tctacctcca ccgtgcgtac
    44581 cgctggctct tcgggtgcga acccgtttgc ctgtatcgca gcaggtattg cttcactgtg
    44641 gggacctgcg cacggtggtg ctaacgaagc ggcgctgaaa atgctggaag aaattagctc
    44701 cgttaaacac attccggaat ttgttcgtcg tgcgaaagat aaaaatgatt ctttccgcct
    44761 gatgggcttc ggtcaccgcg tgtacaaaaa ttacgacccg cgcgccaccg taatgcgtga
    44821 aacctgccat gaagttctga aagagctggg caccaaagat gacctgctgg aagtggctat
    44881 ggagctggaa aacatcgcgc tgaacgaccc gtactttatc gagaagaaac tgtacccgaa
    44941 cgtcgatttc tactctggta tcatcctgaa agcgatgggt attccgtctt ccatgttcac
    45001 cgtcattttc gcaatggcac gtaccgttgg ctggatcgcc cactggagcg aaatgcacag
    45061 ttacggtatg aagattgccc gtccgcgtca gctgtataca ggatatgaaa aacgcgactt
    45121 taaaagcgat atcaagcgtt aatggttgat tgctaagttg taaatatttt aacccgccgt
    45181 tcaaatggcg ggttaatttt tatatgccta aacacaaaaa attgtaaaaa taaaatccac
    45241 tatcggacat tcatatattc taaaaaaaaa tacatcggta taaattataa acaaatagat
    45301 gatttttaat catctatctt cctctcagtt acatgaataa accaccaaat atagcgcacc
    45361 cctataattc ccaatacttt attcatcaaa catttaattc ataccatcaa ttaattactt
    45421 ttacatttaa taaaattaat aatattttta agaaaaacta aaaacattcg acaatcaaat
    45481 aagatattct tgcaccgata tacttttttc ttttctgtta aaggactcag ttatgtttaa
    45541 aggacaaaaa acattggccg cactggccgt atctctgctg ttcacagcac ctgtttatgc
    45601 ggctgatgaa ggatccggtg aaattcactt taaaggtgaa gttattgaag caccgtgtga
    45661 aatacatcag gatgatattg ataaagaggt tgaactcggt caggtgacca ccagccacat
    45721 taatcagtca catcacagcg atgccgttgc tgtcgacctg ctcttagtca actgtgatct
    45781 ggaaaactcc agcaacggtt ccggtggcaa gatttctaaa gttgcagtca cctttgatag
    45841 ctcagcgaaa accaccggcg cagatccgat tctcaacaac accagcacgg gtgaagccac
    45901 cggcgtcggc gtacgtttaa tgaataaaga tcaaagcaat atcgttttag gcaccgctac
    45961 tccagatatc gacctggctc cgacctccag cgaacaaacg ctgaatttct ttgcctggat
    46021 ggaacaaatt gatcaggcta cacctgtaac gccaggcgca gttacggcga atgcaaccta
    46081 cgtgctggat tataaataag tattactttg gcggcgctat cgccgccttt cataaaatat
    46141 ttatcaggta tggacaccgt gaatatttat cgactctctt ttgtatcctg cctggtcgtg
    46201 gcgatgcctt gcgcattggc ggtcgaattc aatctgaatg ttctcgataa atcaatgcgc
    46261 gaccgcattg atatttcatt attaaaggaa aaaggagtca ttgctcccgg tgagtatttt
    46321 gttagcgttg cggtaaataa taaccaaatc agtaacgagc aaaagattaa ctggcacaaa
    46381 aatgacgata aaaccattcc gtgcatcaat gatttactgg tcgataaatt tggcttaaaa
    46441 cctgaagtcc gtcagtcgtt accattgata aatcagtgcg tcgattttag ctcccgacct
    46501 gaaatgctct tcaatttcga tcaagccaat cagcaactga atattagtat tccgcaagcc
    46561 tggctggcgt ggcactcaga aaactggacc ccaccctcca catggaaaga aggtgtcgcc
    46621 ggtgtcctga tggattacaa cttgtttgcc agcagctacc gcccacagga tggcagcagc
    46681 agcactaatc ttaacgccta tggaacagcc ggaatcaaca cgggggcatg gcgcttacgc
    46741 agtgattatc agttgaatca tactgatagc gatgataacc atgaacagtc aggcgaaata
    46801 tcacgtacct atctttttcg cccgttaccg caattaggtt caaaattaac cctcggcgaa
    46861 acggatttta gtcccaatat tttcgacggt ttttcttata ccggcgcggc actggcaagt
    46921 gacgatcgaa tgttgccatg ggaactacgc ggctacgccc cacaaattag cggtattgca
    46981 cagaccaatg ccacggtgac gatcagtcaa tcaggccgcg tcatttacca gaaaaaagtc
    47041 ccaccaggcc catttatcat tgacgacctt aatcagtctg ttcagggcac actggatgtc
    47101 aaagtgacgg aagaagatgg tcgggtgaac aatttccagg tttcagcagc atcgacgccc
    47161 ttcctgactc gtcagggaca ggttcgctat aaactggccg cgggtcagcc acggccctcc
    47221 atgtcacatc aaactgaaaa tgaaaccttt tttagcaatg aagtttcctg ggggatgctg
    47281 tcaaacacct cgctgtacag cggcctgctg ctttctggtg atgactacca ttctgccgca
    47341 atgggtattg ggcaaaatat gctgtggctt ggtgcgctgt cgtttgatgt cacgtgggcc
    47401 agtagccatt ttgatactca gcaggacgag cggggcttaa gctaccgttt taattacagc
    47461 aaacaagtgg atgccactaa cagcacgatt tccctcgccg cttatcgttt ctccgatcgt
    47521 cattttcaca gctacgccaa ctatctggat cacaaataca acgacagcga tgcgcaggac
    47581 gaaaaacaga cgatcagctt atctgtgggc caaccgatta ccccactaaa cctcaatctt
    47641 tacgccaacc tgctacatca aacctggtgg aatgcagacg cctccacgac cgccaacatc
    47701 acggcaggtt ttaatgttga tattggtgac tggagagata tctctatttc tacgtcattc
    47761 aatacaaccc attacgaaga taaagatcgc gacaaccaga tttacctgtc gatttcgctc
    47821 cccttcggta acggtggtcg ggttggctat gacatgcaaa acagtagcca cagcaccaca
    47881 caccgcatgt cgtggaacga tacgctggat gaacgtaata gctggggcat gtctgccgga
    47941 ctgcaatccg accgtcctga caatggagcc caggtgagcg gtaactatca gcacctgagt
    48001 tcagcgggtg agtgggatat ttctggtacc tatgccgcca atgattacag ttccgtcagc
    48061 agcagctgga gcggttcttt caccgcaacc caatatggtg cagcgtttca tcgccgcagc
    48121 tccaccaatg aaccacgcct gatggtcagc accgatggcg tggcagatat tccggttcag
    48181 ggcaatctcg actacaccaa ccattttggc attgcggtgg tgccgttgat ttccagttac
    48241 cagccttcca ccgtggcggt gaacatgaat gacttacccg acggcgtaac agttgcagaa
    48301 aacgttatca aagaaacgtg gattgaaggc gcgataggtt acaaatcact ggcttcccgt
    48361 tccggtaaag acgttaacgt catcattcgc aacgccagcg gtcagtttcc tcccctcgga
    48421 gcggatatcc gccaggatga cagcggcatt agcgtgggga tggttggcga ggaaggacat
    48481 gcctggttaa gcggagtcgc tgaaaatcaa aagtttaccg tggtctgggg tgatagccag
    48541 cattgctcgc tccatcttcc tgaacatatg gaagacaccg caaatcgcct gattttacct
    48601 tgtcattaat taaggaaatg aaaatgacat ttatgaaagg actacccctt ttgttgttgg
    48661 ttgccagtct gtgcagccac gctgcactac aacccgatcg cactcgtatt gtgtttaacg
    48721 ctaatgataa agctaccagt ctgcgggtgg ataatcgtag cgataaactc ccctacctcg
    48781 cttattcctg gctcgaaaat gaaaaagggg agaaaagcga tgatcttctg gtcgccctgc
    48841 cgccgataca acgtctggaa ccgaaagcaa caacacaggt acggatagta aagcaagcct
    48901 cgaccacaaa attaccgggc gatcgcgaaa cgctattttt ctacaatatg cgtgaaattc
    48961 ctcccgcccc ggaaaaaaac agcgaccatg ccgttctgca agtcgccatt cagagtcgta
    49021 tcaaggtgtt ctggcgaccg gctgcattac gcaagaaagc aggagaaaag gtcgaactgc
    49081 agttacaggt cagccagcag ggcaaccaac tgacactgaa aaatcctacc gcgtattatc
    49141 tgaccattgc ctacctggga cgaaatgaaa aaggcgttct ccccggcttc aaaaccgtga
    49201 tggttgcgcc ttttagtacg gtcaacacaa acaccggaaa ttatagcggc agccagtttt
    49261 atctcggtta tatggatgat tacggcgcgt tacgcatgac gacgctcaac tgcagcggac
    49321 aatgccattt acaagccgtg gaggcgaaga aatgagtgct ggcaagggag tgttgctcgt
    49381 catctgtctg ttatttctgt cgttgaagtc tgcgcttgcc ctgaactgct actttggttc
    49441 atccggtggc tcggtagaaa aatcagaagc gattcaacct tttgctgtac caggcaatgc
    49501 caaactcggc gataagatct gggaatctga cgatattaaa ataagatctg ggaatctgac
    49561 gatattaaaa ttcccgtcta ttgtgacaac aataccaacg gcaactttga aagcgaacat
    49621 gtctacgcat gggtaaaccc ctacccgggg gtgcaggatc gttattatca actcggagta
    49681 acttacaatg gcgttgatta tgacgccaac cagggaaaaa gccgcatcga cactaaccag
    49741 tgtatcgaca gtaagaacat tgacatttat acccctgatg ggatggcaga acaaaatttg
    49801 ctcgggtgat cccgctaata ttcacatgtc gcgaaccttt ctcgctcgca tgcggttata
    49861 cgtcaaaata cgagaaatgc cgccgcatga ttatcaaagt acgcttagcg actatatcgt
    49921 cgtgcaattt gacggtgccg gtagcgttaa cgaagaccct actgcccaaa acctgaaata
    49981 tcatattact ggtctggaaa acattcgcgt gctggattgc agcgtcaatt tttccatttc
    50041 accggaaaca cagatgattg attttggtaa atttaatttg ctggatattc gtcgccacac
    50101 aatgtcgaaa acattcagta ttaagacgac aaaatcacaa aatgaccaat gcaccgacgg
    50161 atttaaagtc agttcctcct tttataccga agaaacgttg gttgaagaag ataaagcatt
    50221 gttgattggt aatggtctga agctacgttt gctggatgaa aacgcctcgc cttatacctt
    50281 caataaatat gctgaatacg ccgatttcac cagcgacatg ttggtctacg aaaaaaccta
    50341 tacagctgaa ctttcgtcca ttgcaggtac ccccatcgag gctgggccct tcgatacagt
    50401 ggtgcttttt aaaattaact ataactgatt tgccatgatg ccctttgcag ccggaaaggg
    50461 catctctttc agaaaattgc tgattttccc cttttccttg attgctgtta ttttttccta
    50521 gcccggcttt ctttatgctg tatcgataca ccaaagagaa aataatgagg gagcgaagga
    50581 tgccagtttt gcagtgggga atgttatgtg tgctatcact tctcctttct attggtttcc
    50641 tcgcggtaca tctcccggcg gcgctattac ttgggccgat gatcgccggg atcatcttca
    50701 gtatgcgcgg aattactctg caacttcccc gctccgcttt tctcgccgcg caggccattc
    50761 ttggctgcat gattgcgcaa aacctcactg gttcgattct caccacgctg gccgccaact
    50821 ggccgatcgt gcttgctatt ttactggtga cgctgctttc cagcgccatc gtgggttggt
    50881 tattggtgcg ctatagctca ctgcctggaa ataccggtgc ctggggttct tctccaggcg
    50941 gcgcagcggc aatggtcgct atggcgcaag attacggcgc agatattcgc ctggtggcgt
    51001 ttatgcaata tctgcgagtg ctgttcgtcg cgggggccgc tgttctggtc acccgcatga
    51061 tgctgggtga taacgctgaa gcggttaacc agcaaatcgt ctggttcccg ccagtgagca
    51121 ttaatctcct gcttacgatt ttgctcgcag tcgttgccgg tacggcggga tgcatgctgc
    51181 gtcttccttc cggcacgatg ctcatcccga tgctggcggg cgcagtgctc cagtctggtc
    51241 agctcatcac catcgaacta ccggaatggc tgctggcgat ggcgtatatg gcaattggct
    51301 ggcggattgg tcttggtttc gataaacaaa tcttactgcg ggcactacgc ccgctaccgc
    51361 aaatcctgct gtcgattttt gctctgctgg ctatttgtgc aggtatggcg tggggactga
    51421 cccggtttat gcatattgat tttatgactg cctacctcgc caccagtccc ggcgggcttg
    51481 ataccgtggc ggtcatcgcc gcagggagca atgccgatat ggcgcttatt atggcgatgc
    51541 aaaccctgcg cctgttcagt attttgctga cggggccagc cattgcgcgg tttatttcaa
    51601 cctatgcgcc gaagcggtcg gcctagtgct ggcagcctgg acaccagtaa aacgggcgcg
    51661 aggacaacgt ggttttctca atgatgccgc cacagcgttc gcacagttcg ccatcacgat
    51721 gaaaaacctt aaagcgaaac agcgccccat gatgcttatt ctcatccacc tgcccccgcg
    51781 tagcgtaaga aaaccgagga atctccagta acgcgtgtgc gagtgcatcc agttgcgccg
    51841 cattgagatc tttcgcttta tgatttccag tcaacccaac ctgccagagg atctccaccc
    51901 gcaaataatt gccaagccca gccagaaacg cctgatcgag cagtaatcct gcaaactgac
    51961 ggttacgaaa gcgcggcgac aataaccgtt cctttaccac ctccggcgtc agattcggat
    52021 ccagcacatc gggaccaacg cgttgtaaaa acggatgcgt ggtcagttgt tccggcctca
    52081 acatctcaat atccgaggcg ctataaagca gaatggtttt gtcagccgtt tgcagtttta
    52141 cccgcaatac tcgcgtggtc tgcggctctt cgccggtatc aaccacgcgc cagacgccgt
    52201 aaagctgatt atggctgtag agcgttaaac cgttgggaaa atgagttaac aacgccttac
    52261 cacgcgtttc cacatgggta acgtgttgac cgataagttg tgattgataa gtttttaact
    52321 gcgggaaggc aaaccagaca tcagttagtg gtttgccttt gatcgccgcc tccaggttat
    52381 ccgctgcacg gcggatctcc gggccttcag gcatggtgat atcctttttt cattctttat
    52441 taagcggggt tatgctgcga caacaatccc ctttttggca aatgtagctc gtaatcggcg
    52501 ggcgaacgcc agagcgtgct cgccgtcgcc atgcaggcag accgtttgcg ccgcgaccgt
    52561 tgcccattcg ccggtgatac ttttgactct gccgtgttgc accatctcca gcgtttgcgc
    52621 caacgcctgt tcttcgtttt caatcaacgc gcctggctga cttcgcggca ccagcgagcc
    52681 gtcagcctga taaccgcgat cggcaaacac ttcctcgcgc gttgtcaggc catattgctt
    52741 gcctgcacga atcagctcgc ttcctgccag cccaacgaga accaatgccg gatcgcaagc
    52801 gtataccgct ctggcgatgg cgtctgccag ttgtgcttct ttcgccgcct ggttgtacaa
    52861 catgccgtgc ggtttgacat gacgcattac gccgccttgc gcacgggcaa tcgttgccag
    52921 cgcgccaatt tgatacagcg tctgggcgta aacggtttcc ggcggcagct gcatggcgct
    52981 gcgaccaaaa ttttccctgt cgggaaaact cgggtgagcg ccaatcgcga caccattttt
    53041 tattgcttca cgcacgcaag cctgcatggt ttgcgcatcg cctgcatgaa atccacaagc
    53101 aatattggca gaggaaacca gcgttaatag ctctgcgtcg ctggcgcagc cttcgcccag
    53161 atcggcgttc aggtcaattt tcattgtgca gccgccacgc taattgttca aaataacgtt
    53221 gctgatcttg ccgcgctttc agtgcctctt ccagtgaaca ctggacaaaa tgaatcggct
    53281 gaccgagcgg aatttgcgcc agatggtaca tatcagcctc aatgatacag gcaatacgtg
    53341 gataaccacc ggtggtctgt gcgtcgttca tcaacacaat cggctgcccg ttatgcggca
    53401 cctgcaccac gctcggtaac aaaccgtgag ataacagttc gcgatcggtg atgcgtttta
    53461 aaatttgccc ctgtaaacga tagcccattc ggttactttg cgagctaagc tgccagggcg
    53521 aacgccagaa tgcatcctgc gaggcgcgat cgaactcatg atattccggc cccggcaagg
    53581 cgcgaatgcg gttgccccac agcagctgtt taacgccctg cgcttccata aaatcacgct
    53641 tcgatttgcc aatcgggagt cagtcaccat ccttcagtaa acggccttcc agcccgccaa
    53701 tccccacttt gagatcggtg ctgcatgacc ccattaccgg cggaacatca ataccacccg
    53761 cgaccgccag ataactgcgc atcccgtgct gcgggcgttt aagcgttaaa cgctggcctg
    53821 ctttcatcgg caatcgccag ccggtccaga cggcgttatc atccaaccgc gcttcgcaac
    53881 ctgcacccgt cagggcaaac cacccatcag tttcgaactc aactgttaac tgaccgagcg
    53941 tgatctccag cgcgggggca ttggcgtcat tacccaccag caggttagca atgcgcagcg
    54001 caggcatatc cagtgcgcca cagtggctga tacccgactg gcaaaaaccg tgacgaccgc
    54061 catcctgcac agtggtgtac atgcccgcac gaataatctt cagcatattc cctccttctg
    54121 cggtacaaat cgcacgctgt ctcccggacg taataagatg ggttcgtcac gcgccggatc
    54181 aaacaggctg agtgaggtat gaccaatcaa ctgccagcca cccggcgttg ccagcggata
    54241 aacaccagtc tgcggcccgc cgatcccgac agaacctgcc ggaacgagca agcgcggttc
    54301 agcgcgccgt ggcgtgtgta gttgttccgg caaactcccg agatacggga agcccggttg
    54361 aaaacctaaa aaccagacca cgtattccac ggaggagtgc aattcaacaa cctgtttttc
    54421 gctcaacccg caatacgccg cgaccaccgc caaatccggt ccgcctgcac caccgtaaac
    54481 caccggaatt tcaataaagc gagactcagg ttccagggcc tcgctctcct cccaccagcg
    54541 ttgcaaacgc tctatggcat ccagcgccag cgactcagga ttacgcaaaa tcaccgtgat
    54601 attgttcatg ccgggaatgg cttcaaccac attcggcata tccaccagac gctgcgccag
    54661 tcgccagatc cgtttctggc tagccagcgt caccggcggt tccagttcca gcactaccgc
    54721 cgtttcacct atcagataac aacgcgctcg ttgcactttc gatccctctg attattcgtt
    54781 atgcaggatt aggaatatca ataaaggtca catcaagatc ggtattttca ttcagccact
    54841 cgctcaatgc gcgaatacca ccgcgttcag tggcatggtg acctgcagca taaaaatgca
    54901 atccctgctc gcgggctgaa tgaatggtct gttcagaaac ttcgccagta ataaaagcat
    54961 ccacgccaaa acgcgcggcg ctatcgataa aactttgccc gccgcccgtg caccaggcga
    55021 cgcgctggac cacctcaggt ccggtatcgc cacaccataa cggcttacgt cccagacgcg
    55081 cttcaatcca ggaggctagt tccattcccg gcacaggcat ggtcagctct ccccacggca
    55141 ccaacggctc aatttcgccc atgaccgtga tccccagtaa cgccgccagc tgtgcgttat
    55201 tgcccagctc aggatgtgcg tcaagcggca aatgccagcc atacaggttg atatcattcg
    55261 ccagcaacgt ttttaaacgg ttacgcttca ttccgcgaat gaccggagac tctcctttcc
    55321 agaagtagcc atgatgcaca atgaccgcat ctgcgcccag acgcactgcc tcatcgagca
    55381 aagcctggct ggcggtgaca ccggtaacaa ttttttgcac cgtctctttg ccttccacct
    55441 gcaaaccatt cggcgcgtaa tcactaatcg ccgcgctgtt cagtttttcg ttaatcagtt
    55501 gttccagttc ggtgtttttc atcttcgcgc cttccgtatt ctgatagcta caccgttaac
    55561 atagcgttac atcgcctgtt cgtcgatagc caatcttttg acatattaag aaacaaaccc
    55621 gacgccaaaa gacgcaaatt gtttatatca tcacgcttca gctaatgtat atttatgcaa
    55681 tcagacagca tattgcataa ataatcagat ctgtatcaaa gttttaactt taaggaaaca
    55741 caagcacgcc gcgcaactat tacgtgacgt tgccgtcaat tgtggaatta ttatgaataa
    55801 acacgcatca caaccgcgcg ctatttacta tgtcgttgcg ctgcaaatct gggaatattt
    55861 tagcttttac ggcatgcgtg ccctgctgat tctctatctc accaatcaac taaaatacaa
    55921 cgatactcac gcctacgagt tatttagcgc ctactgttcg ctggtgtatg tcacgccaat
    55981 cctcggtggc tttttggcgg ataaagttct cggcaatcgc atggcggtga tgctgggggc
    56041 gttgttgatg gcgatcggtc atgtggtgct gggtgccagt gagatccatc cgtcattcct
    56101 ctatctgtcc ctggcgatta tcgtctgcgg ctatggcctg tttaaatcta acgtaagctg
    56161 tctgctcggc gagctgtatg agccaaccga tccgcgtcgt gatggcggtt tctcgctgat
    56221 gtatgcggcg ggtaacgtgg gatctattat cgcacctatc gcctgtggtt acgcccagga
    56281 agagtacagc tgggcgatgg gctttggcct ggcggcggtt ggcatgatcg cgggtctggt
    56341 cattttctta tgtggcaatc gtcatttcac tcatacccgc ggcgttaaca aaaaagtact
    56401 gcgtgcgaca aactttctcc tgccgaactg gggatggctg ctggttctgc tggtggcaac
    56461 gcctgcactg attaccgtgc tgttctggaa agagtggtcg gtatacgcct taattgtcgc
    56521 gactattatt ggtctgggtg tactggcaaa aatttatcgc aaagcagaaa accagaaaca
    56581 gcggaaggag ctggggctga ttgtgacgct caccttcttc agtatgttgt tctgggcctt
    56641 cgcacaacag ggcggtagct cgattagcct ttatatcgac cgcttcgtta accgcgatat
    56701 gtttggttat accgttccga ccgcgatgtt ccagtcgatt aatgccttcg cagttatgct
    56761 gtgcggtgtg ttcctggcgt gggtggttaa agaaagcgtc gcaggtaatc gtaccgtgcg
    56821 catctgggga aaatttgctc ttggccttgg cctgatgagc gccggattct gcattctgac
    56881 cttaagcgcc cgctggtccg caatgtatgg tcactcttct ctgccactga tggtgttagg
    56941 cctcgcggtg atgggctttg cggaactgtt tatcgacccg gttgccatgt cgcaaattac
    57001 gcgcattgaa attccgggcg tgaccggcgt attaaccggc atctacatgc tgctttctgg
    57061 cgcgatcgcg aactatctgg cgggcgtgat tgccgatcag acatcgcagg cttcgtttga
    57121 tgcttccggg gcgatcaact actccatcaa tgcatatatt gaagtatttg atcaaattac
    57181 ctggggcgca ctggcgtgtg taggactggt actgatgatt tggctgtatc aggcgctgaa
    57241 attcagaaac cgcgcgctgg cgctggagtc ataataggcc agcaccgatg cctgatgcga
    57301 cgcttgacgc gtcttatcag gcctacgtat ttcctgcaat ttattgaatt tgcacaaatt
    57361 tgtaggctgg attaggcgtt cacgccgcat ccggcatctg ggctcgattg cctgacgcgt
    57421 ctgttatttc cccttccgcg ccgcctcata cgctgccaac gtttgtactc tcgcctcttt
    57481 gtgctcgact atcggttgcg gataatccag cttcacacct gctttctgcg cccacttcca
    57541 cggctcatgc accgctttcc ctggtacatc gcgcagctcc ggtaaccatc gacgaataaa
    57601 ctcgccctca cggtcaaatt tctcgccctg agttatcgga ttgaaaatac gaaaatacgg
    57661 cgcagcatca gtacccgtag aagccgccca ctgccagcca ccgttattag ctgccaaatc
    57721 accatcaatc agctgcgaca tgaaatatcg ctcgccttcg cgccagtcga tcaacaaatc
    57781 tttcaccaga aaactggctg taatcatccg tagccggtta tgcatccagc cagtgctgtt
    57841 aagctgacgc atagcggcat caataatcgg gtatcccgtt ttgcctttct gccaggcctg
    57901 taaatgtgcg ggattgcttt gccactgcac acgatccgtc caggcaataa atggacgatg
    57961 tttacacaac gaggggtaat acgtcatcag atgacggtag aactcgcgcc agatcagctc
    58021 actaagccag acactaccgg ccccaccgtc cagcgcctgc ggctgttccg ccaacaagcg
    58081 atgcaaacac tggcgaggcg ataacccgcc cgttgccaag ctggcggaca aacggctggt
    58141 gccttccact gccggaaaat ctcgttgttg ctcatactct ccagcaccgt tctcgcaaaa
    58201 ctggcgtaat tgcgcaatcg ccgctttttc ttccaccggg aaatgcgcag tatcgaaaga
    58261 ctgacgagga taattcagcg taatggatgg cgcgggcttt atcgatccgc tactacgaac
    58321 ttttggtgca gcgacgcact ccggcatccc ttcccgcagc cgtttcagcc aggcattctt
    58381 aaaaggcgta aagactttgt acatctcgtg attgccggtc atcaccgccc ccggcggcag
    58441 gatcacgctg tcatcaaatc cttcacacac cacgttacgc agcgctcttt caacgtccac
    58501 atcccgcgct cgctcattca cctcatactg atagttataa aaaagatgag taacgctgtt
    58561 ttccgcgcac acctgtttaa ctatttcgac actggcgaca aagtcatcca cttcacggaa
    58621 caataaagga ataccttttt ccgccagcgc gatttgtagc ccattcagtt gagcattgat
    58681 aagttcagcc tgacgcggcg acatgttatg cgtctcccac tggcgtggtg tagcgatata
    58741 cagcgccagc acgcgtgcag acgaattgcg gcaggcggca gccagtgcga gattatcgtg
    58801 cagacgtaaa tcctggcgaa accaggccag atgggtagtc ataaaactcc tgaatggcgc
    58861 aagttaatca cacgcggcca aagttcgaaa taacgctgcc ctgaaagcca ggcgtcagga
    58921 taaagcgcca tatagtgttt gatacgcatc agcggcgcaa gaagtggttg ctcgccacgg
    58981 cgataactgt cgataagcgc agccagctgc tggcgttctg tggaatcaat atgcgggcga
    59041 aaataaccct gaacgtgcat caaaacgttg gtgtgatcgc gcgggttggc gggatgagaa
    59101 agcaaaacaa ttacgcgttg gcggtactgg ttgtaaaaat catccagatt ttgccactga
    59161 tgaatatcag ccacaaacgg gccaagtttg cggtactcgg gctgagaatg ggccagaaag
    59221 acgagtttat agcggctgtg ataatcgaga agcgccccgc gcgttaagcc atgttctttc
    59281 agtcgatgta actcatgaag ggcgaagata cggattaggg tgctgtcatc aaatcgttgt
    59341 agattcatta ctgctcctga acggtataca ggagtaagtg taggcgttgg taaatgatgt
    59401 ttgtgaagtt aatccgctag cagggcaaga ttacgaaagc gcgctccccg caagcaacta
    59461 atcaaggtag ttaatcatgg ctgaacacga tattacgcca gaagtcacca tttccaaaac
    59521 acagcgccgc tataaagtcg gctatgtcag agcccgccac caggaccgca aaaccggtat
    59581 agcccggtat tattcacaac atcccagcct gcaccttaag ggcgactggc tgaaagaggc
    59641 cggatttgat accggacggg gtgtcacggt gaagatttcg gaggggggcc tgaccatcat
    59701 cgctgacagt gacgaggtgc aggaactgcg ggatgggatg tttagtgtgc tgaacgaaag
    59761 ttaaatatta aaaaagctgg gaagatttaa aaccttccta gctttttatt ctgtcttatt
    59821 ataatggata ctcatcatct tccgatgaat ttataaattg ctccaagttt acatgaagag
    59881 tacttaacct agtttttaac tcattaagtg aagcgataat ttcatatttt ccaacataat
    59941 catatgatgg atcgtattca ttatctatgt cagaagctaa aaatgatgat attcgtagtt
    60001 taaatttagc ttcatcttta ttacattcat tggcataagg tgaatgccct atcatagcat
    60061 attcatctaa tctaaaagga taagatggtt ttaattcatc gatcaatgaa atttcaatcc
    60121 atgttctata tccaggcatt aatggactaa tttctttgag caaaaccttt ccacttaata
    60181 atgtattgag attgttcatt attatgtctc ccacgggaaa aatatatgtc taacacttcc
    60241 accatgttga tgaacttgta aatgtggtaa atcaccatca ggatttccta gtccatgacc
    60301 tgacactcta tatgaaggtt ttgttacaga aggatctttt atatcatccc aatgatatga
    60361 acctgctttt gttttatttc cagtaagata ttccattaag ttcttagtgc tggggcctga
    60421 ctcacctgtc atatttttat aattatgacc taaatatcta tccataaaca attgttcagc
    60481 gtcacttcta gacctaacag taactttggt ttcactacta tctaataaac gattcgcttt
    60541 ttggttacta acagcacatg gagttaatcc aagtggatca gcatatttta gaggattagg
    60601 tgcataactg tagagattta aaccgcctct caaccctatc gggtcagtta ccatgaaccg
    60661 cccgacatcc ggatcatagt acctgtgcag attgtagtgc agccccgttt ccctgtcgag
    60721 gtactgtccc tggtagcgca ggttctgctc caccccgccg tggtccttct cctgtatcgt
    60781 gttaccccag acctgatacc ccgtctccca cacgattttc ccgtcgctgt ccgtcatctc
    60841 ttccggcgcg ccgttcacat ccgtgtgaaa atacagtatc cggttcgggc cctctttgcc
    60901 cgccttatcc acccgcgcca gcggttcata actcccctgg tccctgtaga tgtacaggct
    60961 gcctgttccc tccggcctgc tctcctgcgc cagccgcatt ccgtcccata aaaattttgt
    61021 ccggttgtac ggcttaccgt cagggctgat gtgctgtttt tccgtccgcc tccccagtgc
    61081 atcgtacaca taccggtatg tcccccgcgc tgtcgtcact tccaccatcc ggtgttccgc
    61141 atcataacgg taactctgcg ttccgctccg gccctccttt tccaccgcac ggccaaattc
    61201 gtcataacgg tagtgcacac cacggtaaga catcagccgg ttgaacggca ccacgctgtt
    61261 ttctgccgtg gccctttccc ccgcgcgcct ccccagcagg tttcccgccg cgtcgtactg
    61321 ccagctcgcc agatacccct catcctccga acgacacgcc gtgatgcgcc ccgtggggtc
    61381 gtagaagtaa tccgtttccc cccgccggct gtgccgcgtc ttcagcagtt catcctcgcc
    61441 actgtagctg tactgccggt ccgtgaacgt ctccggtacc accccgcgca tcccgctgta
    61501 tgtctccctg cgcttcagcc gcccgccggg gtcgtaaagc tgccgcgtgc tcagtcgccc
    61561 ctggcttctg agcacttcac ggtgcaggtg gtcgcgcgtg aactcgctga tgacctcccg
    61621 gtccagattt atctgctgga gatggccgct gccgtagtac agatggcgct gcgttctgcc
    61681 gtccggaaag gtggtggcag tccggttgcc cagtttatcg tactggtggg tcaggatgcc
    61741 ctgcgccgtc tcctcgctca gcatccgccc cagtctgtcg tagctgaagc ggataacttc
    61801 cggcgcgtca gtttcgtgcc gctggcgacg gatttccagt agctggtctg ccgcatcatg
    61861 gacgtattcc gtacggccat cggctgtctc ttttgccgtg agccggcccg ccgcatcacg
    61921 cgccagccgg tggcggattt caccgccccg gtccccggtg aacaccactt ccgtgaccgc
    61981 gttcagcgcg ttgtagcggt acgactggcg gcagccgtcc gggtttatct gctccgccag
    62041 acggtccagc acatcatggc ggaaccggta gcgttctccg ttctcgttcg tgagcgtgac
    62101 cagacgcccg taagcgtcgt acccgaatgc cgtcctgcgc cccatcgcgt ccgtccggct
    62161 gcgcacccgg ccctgcccgt cccgctgcca gcgcgttatc ctccccatcg ggctggtgta
    62221 ctttaccagc ctgcccgccg cgtctgactg gtaattcacc gtgcggccgt ccgccagtat
    62281 cacctccgtc agatgaccgc tgtcgtcata cccgtagcgc gtgctgtggc tctgcgcatc
    62341 cgtcattctg ataagcctcg tgcgttcgtc gtagaaccac gccgtctgcg agccggaaca
    62401 gtccgtgtgc cgcatgagct gcccgtcttc attccactgg aggtatttat tccccccgcg
    62461 cgcatccgtt atctgcacca cctagccgtg gtggtcatac tgataccggg tgttctgctg
    62521 cagcgggtcg gtcacggcaa gaaggttgcc gcgttcatcg tacacacagc gccaggtgtt
    62581 tcccgccgcg tccacctccg tttccggctg gtgccacacc gggtgccact gcgcctgctc
    62641 cacccgcccc agcgggtcgt gcgtctccgt gatatgcccc tgcaggtcat acacatagcg
    62701 ccatttgccg ccctgagggt cggtcatccc cagcaacagt cgttcttcgt cgctccagcg
    62761 gaatgtcgtc acctgtccgg cttcgtcctg gtacgccgtc acctgattct gcgcatccca
    62821 ctggtgataa cgtgtgcgcc ccagtccgtc cgtcacgcac agcctgcgct gcgccaggtc
    62881 ccacgccagc cggtactgtt caccgctgtc cgtgtggtgc cccgtcaccc gccatgccgc
    62941 gtcatccgga ccttcatacc agccccactg gtaataatac ccttcgccgc ccggctggcg
    63001 gtgcagcgtc atgcagtggt tttcgtcgta agcaaactca cgcagcaggt gccccgtgct
    63061 gtcgtacacg ccgttcaggt ggccggcctc gtcatagctg taacgacgca gcagtaaagg
    63121 tgcagtctca gggaaatgct gataaatctg tgtcacccgc cggggatgcg ccgccagttc
    63181 gtaatacaga cggatgcacg gacgctgctg ttcgccgctg atttccacaa tgcggccccg
    63241 gtcgtcgtag tacagattca gacagttgct gttacggtcg cccagcattt tcagccgctg
    63301 gcgctgcggg tggtgcgggt cagcctcaaa cagaaaacac tgcccgtcat cgctgctgat
    63361 aagccagtgc ccctgctcac cacgccttac cgccagccct tcaccggggc tgtaaaaccc
    63421 gccccccggc agtacggctt ccatatccaa gcgtcgcccc atgcccgaca caaaggtcat
    63481 gcagttttcg tctgcgctac ccggtgtgca cacgaggctg actttataca ttacactcca
    63541 gccctgacca aacattcctt ccgtacgttc gtcacgactg ctgtacacgc gctgccagtc
    63601 cagcggaaag tggcccggca gactgaaatc gaagtcatcg tcatcggcca ggtatttggc
    63661 cccggtggcg gcattgaccg gatgcggaag ggcactggtg gccttctgcg tcagcatgct
    63721 catcccgaca ctggcagcaa aacaggcgat tttagaaagc attttgcccg ggcgaagaaa
    63781 catcagcgcc acggttgcca ccagggttat ctgcgacctg ccgcttctga tatccctgac
    63841 caccagtaac ggaccgccaa tgcgcacatc cggtgacaca aacgccccgt tccccggctc
    63901 attcaccacc ctggcctcgc aggtacagcg tgccccgctg cgtgtcgccg gctggctgtt
    63961 gatacgcaca tcccgcgcgc cttccgcgat atattccgct gcgggcggct cgtcatcgcc
    64021 tttgaacaga ttactgatgc tgccaataac cgcattcact ccctgaaagg cggtttccag
    64081 cataccgccc ggattcagca ggttaagggc cgttcccagg ggggcatcca gaaaggcggc
    64141 ttttttttgt gcgagaaact gcgtcagtga ggaaggatgt ttatggcagt cgattttatc
    64201 gtcaggggtc ggggtggatc ccggggccgc actggccaca gtgggctgcc atagttccga
    64261 cacaaaatgt gtcacgccat ccaccatttc agaacgccct tcttccgtac tgagctggtc
    64321 gtgcagagcc cgggacatca gcacaggccc tgtcaccggg ttaaccgcca gggctatggc
    64381 ccccgccatt tccagatatt ccccggccac atcccggaac gttctggcat cctcctgacg
    64441 ctgcgcttct tcctgttctg ccttcgcctg cgcttcctgc gcctctatgt cctgccgcga
    64501 tgccgcagtc gccgctgccc gcgcggcagg tttgctgttg atccatgtgt caccagagcc
    64561 ggtgaggatg taaccttcaa tctgaggcgg gaaaagcata tttgcgaggg ctgaacaggc
    64621 ttcgctgacc ttttctgtca cgccggtgta caccatcgcc acacccgcca caaaaccggt
    64681 cagcacgacg gccgtgcacc ccatcgtgat ccccactgac gtcgccagta acgaggttgt
    64741 gccgacaatg agggcatcaa taacgacatt tgcggccacc tccagcaccg cccccagcac
    64801 atctgccata aacgacgtat gcagcagggc atcaccctgt cttgccgccc agaatgcctc
    64861 ttccatgtcc cgttccctgt atgattaatt cgtcctgact tcctgccgca tcctgaagcg
    64921 cagattcttt ttaatttcag cccagcgcgt catatctgcc ggggtcagcg gtgtggttct
    64981 ggcgtaggtg aaggccatca tgacccgctc acccggcaat acaaatgcca cctgacactg
    65041 gtacacctgc tcgttacccc ggagaaactg gctctgtatt tccaaaccgc ctcgttgaga
    65101 taatattccg tcataatgta aatagtccgt tttaataaag gaattattta ttgacaggtt
    65161 ctgttttttt aatcatgaat gtaataccca tcaccgcttt acagcacact ttatctttcc
    65221 agccataatc actaataata ttaagcaatt agcaatattt acaacgacca gagcggatac
    65281 atagttatgt atttaacaat cccaatatat tcactatgca aataaaacca ctagtaacag
    65341 acacgctacc gcctccatat aaaaatggac accttataaa attgtatctt gcatagaaaa
    65401 agctgaaata tcggatgcgt tttaattatc aactataaat ctataagctt tttattaaca
    65461 a
//
LOCUS       DAJTQY010000011        60420 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.011, whole genome shotgun sequence.
ACCESSION   DAJTQY010000011 DAJTQY010000000
VERSION     DAJTQY010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 60420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60420
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.011"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            <1..323
                     /locus_tag="OO349_000717"
     CDS             <1..323
                     /locus_tag="OO349_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5865898.1"
                     /translation="KVGTENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPND
                     SNSVYKDYDPRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQ
                     VTYP"
     gene            436..1230
                     /gene="panB"
                     /locus_tag="OO349_000718"
     CDS             436..1230
                     /gene="panB"
                     /locus_tag="OO349_000718"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HCR5865899.1"
                     /translation="MKPTTIASLQKYKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGNEAS
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            1242..2093
                     /gene="panC"
                     /locus_tag="OO349_000719"
     CDS             1242..2093
                     /gene="panC"
                     /locus_tag="OO349_000719"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HCR5865900.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHNGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(2167..3069)
                     /gene="rpnC"
                     /locus_tag="OO349_000720"
     CDS             complement(2167..3069)
                     /gene="rpnC"
                     /locus_tag="OO349_000720"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HCR5865901.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEECLKGHSTDVLYSMQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEAGHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGKSMMTLAQWFEEKGIEKGIQQGRQEERQEFALRLLSKG
                     MSREDVAEMANLPLAEIDKVINLI"
     gene            3343..3723
                     /gene="panD"
                     /locus_tag="OO349_000721"
     CDS             3343..3723
                     /gene="panD"
                     /locus_tag="OO349_000721"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HCR5865902.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(3727..4956)
                     /locus_tag="OO349_000722"
     CDS             complement(3727..4956)
                     /locus_tag="OO349_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCR5865903.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQISDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNTINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATVFIVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLEKMSRLVSNQPQG"
     gene            complement(5020..5460)
                     /locus_tag="OO349_000723"
     CDS             complement(5020..5460)
                     /locus_tag="OO349_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR5865904.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRECIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5565..6335)
                     /locus_tag="OO349_000724"
     CDS             complement(5565..6335)
                     /locus_tag="OO349_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5865905.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(6332..7258)
                     /locus_tag="OO349_000725"
     CDS             complement(6332..7258)
                     /locus_tag="OO349_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5865906.1"
                     /translation="MTIALELQQLKKNYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STAIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVEHKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7367..8029
                     /gene="can"
                     /locus_tag="OO349_000726"
     CDS             7367..8029
                     /gene="can"
                     /locus_tag="OO349_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HCR5865907.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(8070..8606)
                     /gene="hpt"
                     /locus_tag="OO349_000727"
     CDS             complement(8070..8606)
                     /gene="hpt"
                     /locus_tag="OO349_000727"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HCR5865908.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            8812..11202
                     /gene="gcd"
                     /locus_tag="OO349_000728"
     CDS             8812..11202
                     /gene="gcd"
                     /locus_tag="OO349_000728"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HCR5865909.1"
                     /translation="MAINNTASRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQIHADNVHKLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPIIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLLPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(11249..12799)
                     /gene="cueO"
                     /locus_tag="OO349_000729"
     CDS             complement(11249..12799)
                     /gene="cueO"
                     /locus_tag="OO349_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HCR5865910.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLAEETTLHW
                     HGLEVPGDVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFSADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            12965..13312
                     /locus_tag="OO349_000730"
     CDS             12965..13312
                     /locus_tag="OO349_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HCR5865911.1"
                     /translation="MKTFFRTVLFGSLIAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLSNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13418..14284
                     /gene="speE"
                     /locus_tag="OO349_000731"
     CDS             13418..14284
                     /gene="speE"
                     /locus_tag="OO349_000731"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HCR5865912.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFQLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            14300..15094
                     /gene="speD"
                     /locus_tag="OO349_000732"
     CDS             14300..15094
                     /gene="speD"
                     /locus_tag="OO349_000732"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HCR5865913.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(15132..15494)
                     /locus_tag="OO349_000733"
     CDS             complement(15132..15494)
                     /locus_tag="OO349_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HCR5865914.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDAEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(15669..18266)
                     /gene="acnB"
                     /locus_tag="OO349_000734"
     CDS             complement(15669..18266)
                     /gene="acnB"
                     /locus_tag="OO349_000734"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019080489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HCR5865915.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVNVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLSSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLAEIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            18621..20474
                     /locus_tag="OO349_000735"
                     /pseudo
     CDS             18621..20474
                     /locus_tag="OO349_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
     gene            complement(20545..21969)
                     /gene="lpdA"
                     /locus_tag="OO349_000736"
     CDS             complement(20545..21969)
                     /gene="lpdA"
                     /locus_tag="OO349_000736"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HCR5865916.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(22294..24174)
                     /gene="aceF"
                     /locus_tag="OO349_000737"
     CDS             complement(22294..24174)
                     /gene="aceF"
                     /locus_tag="OO349_000737"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HCR5865917.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEATPAATPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAKAEGKSEFAENDAYVHATPLIRRLAR
                     EFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKVDFSK
                     FGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAKRKLD
                     VKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVVPVFK
                     DVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVNAPEV
                     AILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSDIRRL
                     VM"
     gene            complement(24189..26852)
                     /gene="aceE"
                     /locus_tag="OO349_000738"
     CDS             complement(24189..26852)
                     /gene="aceE"
                     /locus_tag="OO349_000738"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HCR5865918.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKELSTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(27013..27777)
                     /gene="pdhR"
                     /locus_tag="OO349_000739"
     CDS             complement(27013..27777)
                     /gene="pdhR"
                     /locus_tag="OO349_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HCR5865919.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            28318..29691
                     /gene="aroP"
                     /locus_tag="OO349_000740"
     CDS             28318..29691
                     /gene="aroP"
                     /locus_tag="OO349_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HCR5865920.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWTMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(29734..30588)
                     /gene="ampE"
                     /locus_tag="OO349_000741"
     CDS             complement(29734..30588)
                     /gene="ampE"
                     /locus_tag="OO349_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HCR5865921.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(30585..31136)
                     /gene="ampD"
                     /locus_tag="OO349_000742"
     CDS             complement(30585..31136)
                     /gene="ampD"
                     /locus_tag="OO349_000742"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HCR5865922.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRLDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            31224..32117
                     /gene="nadC"
                     /locus_tag="OO349_000743"
     CDS             31224..32117
                     /gene="nadC"
                     /locus_tag="OO349_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HCR5865923.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            32320..32760
                     /gene="ppdD"
                     /locus_tag="OO349_000744"
     CDS             32320..32760
                     /gene="ppdD"
                     /locus_tag="OO349_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HCR5865924.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSSIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            32770..33917
                     /gene="tadA"
                     /locus_tag="OO349_000745"
                     /pseudo
     CDS             32770..33917
                     /gene="tadA"
                     /locus_tag="OO349_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
     gene            33907..35109
                     /gene="hofC"
                     /locus_tag="OO349_000746"
     CDS             33907..35109
                     /gene="hofC"
                     /locus_tag="OO349_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HCR5865925.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRTLLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEAFPPLYQAMIRTGELTGKLDECCFELARQQKSQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWGW
                     LLVLFGFLLAIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(35144..36187)
                     /gene="guaC"
                     /locus_tag="OO349_000747"
     CDS             complement(35144..36187)
                     /gene="guaC"
                     /locus_tag="OO349_000747"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HCR5865926.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHFSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGQVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVLEQENRIFNNL"
     gene            complement(36349..36387)
                     /locus_tag="OO349_000748"
     CDS             complement(36349..36387)
                     /locus_tag="OO349_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5865927.1"
                     /translation="MIIKTLKMSARK"
     gene            36412..37032
                     /gene="coaE"
                     /locus_tag="OO349_000749"
     CDS             36412..37032
                     /gene="coaE"
                     /locus_tag="OO349_000749"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HCR5865928.1"
                     /translation="MGYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGENMIAADGTLQRRALRERIFANPEEKNWLNDLLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            37032..37775
                     /gene="zapD"
                     /locus_tag="OO349_000750"
     CDS             37032..37775
                     /gene="zapD"
                     /locus_tag="OO349_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HCR5865929.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELNRQQRKLQTWIGVPGVDQSRIEALIQQLKAAVSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            37785..37982
                     /gene="yacG"
                     /locus_tag="OO349_000751"
     CDS             37785..37982
                     /gene="yacG"
                     /locus_tag="OO349_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HCR5865930.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(38082..38471)
                     /gene="mutT"
                     /locus_tag="OO349_000752"
     CDS             complement(38082..38471)
                     /gene="mutT"
                     /locus_tag="OO349_000752"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HCR5865931.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGVPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(38531..41236)
                     /gene="secA"
                     /locus_tag="OO349_000753"
     CDS             complement(38531..41236)
                     /gene="secA"
                     /locus_tag="OO349_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HCR5865932.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(41298..41810)
                     /gene="secM"
                     /locus_tag="OO349_000754"
     CDS             complement(41298..41810)
                     /gene="secM"
                     /locus_tag="OO349_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HCR5865933.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(42041..42958)
                     /gene="lpxC"
                     /locus_tag="OO349_000755"
     CDS             complement(42041..42958)
                     /gene="lpxC"
                     /locus_tag="OO349_000755"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HCR5865934.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(43059..44210)
                     /gene="ftsZ"
                     /locus_tag="OO349_000756"
     CDS             complement(43059..44210)
                     /gene="ftsZ"
                     /locus_tag="OO349_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HCR5865935.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(44271..45533)
                     /gene="ftsA"
                     /locus_tag="OO349_000757"
     CDS             complement(44271..45533)
                     /gene="ftsA"
                     /locus_tag="OO349_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HCR5865936.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(45530..46360)
                     /gene="ftsQ"
                     /locus_tag="OO349_000758"
     CDS             complement(45530..46360)
                     /gene="ftsQ"
                     /locus_tag="OO349_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HCR5865937.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLSWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLVRFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(46362..47282)
                     /gene="ddlB"
                     /locus_tag="OO349_000759"
     CDS             complement(46362..47282)
                     /gene="ddlB"
                     /locus_tag="OO349_000759"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCR5865938.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSIDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(47275..48750)
                     /gene="murC"
                     /locus_tag="OO349_000760"
     CDS             complement(47275..48750)
                     /gene="murC"
                     /locus_tag="OO349_000760"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HCR5865939.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLLNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(48804..49871)
                     /gene="murG"
                     /locus_tag="OO349_000761"
     CDS             complement(48804..49871)
                     /gene="murG"
                     /locus_tag="OO349_000761"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HCR5865940.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHYLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(49868..51112)
                     /gene="ftsW"
                     /locus_tag="OO349_000762"
     CDS             complement(49868..51112)
                     /gene="ftsW"
                     /locus_tag="OO349_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HCR5865941.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSAVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(51112..52428)
                     /gene="murD"
                     /locus_tag="OO349_000763"
     CDS             complement(51112..52428)
                     /gene="murD"
                     /locus_tag="OO349_000763"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HCR5865942.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALVA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLVRYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(52431..53513)
                     /gene="mraY"
                     /locus_tag="OO349_000764"
     CDS             complement(52431..53513)
                     /gene="mraY"
                     /locus_tag="OO349_000764"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HCR5865943.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(53507..54865)
                     /gene="murF"
                     /locus_tag="OO349_000765"
     CDS             complement(53507..54865)
                     /gene="murF"
                     /locus_tag="OO349_000765"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HCR5865944.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGMEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSMGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(54862..56349)
                     /gene="murE"
                     /locus_tag="OO349_000766"
     CDS             complement(54862..56349)
                     /gene="murE"
                     /locus_tag="OO349_000766"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HCR5865945.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSCVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFPASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGGIA"
     gene            complement(56336..58102)
                     /gene="ftsI"
                     /locus_tag="OO349_000767"
     CDS             complement(56336..58102)
                     /gene="ftsI"
                     /locus_tag="OO349_000767"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HCR5865946.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNSDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKESMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALTMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(58118..58483)
                     /gene="ftsL"
                     /locus_tag="OO349_000768"
     CDS             complement(58118..58483)
                     /gene="ftsL"
                     /locus_tag="OO349_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HCR5865947.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(58480..59421)
                     /gene="rsmH"
                     /locus_tag="OO349_000769"
     CDS             complement(58480..59421)
                     /gene="rsmH"
                     /locus_tag="OO349_000769"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HCR5865948.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEEHF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(59423..59881)
                     /gene="mraZ"
                     /locus_tag="OO349_000770"
     CDS             complement(59423..59881)
                     /gene="mraZ"
                     /locus_tag="OO349_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HCR5865949.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLLSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
BASE COUNT        14477 a        16733 c        14846 g        14364 t
ORIGIN      
        1 caaaggttgg tactgaaaac gggcaactcc ttggtaatac tctcacaggt aatgatgcag
       61 caaaaggagt cggtgtactc atagaaggtt tagcaactag taaaaatcct ctaatgacat
      121 tgaaacctaa tgattcaaat tctgtttata aagattacga cccgagaggc aaagacgata
      181 caacaggagg agtttacccg gatcaagata caggtataac ataccctctc catttccagg
      241 ccacgctaca acaggatgga actataccaa tagaagctgg tgagtttaaa gccaccagta
      301 ctttccaggt aacctaccct taataagtcc accgcaccgc catcatctgg cggtgcggta
      361 attgataaat cattgccccc accaatgtga ctgataaaat gccaggcaac acgacatcat
      421 ttatcaggat acgttatgaa accgaccacc attgcttcac ttcagaaata taagcaggat
      481 aaaaagcgtt tcgcgaccat caccgcttac gactacagct tcgcgaaact atttgctgat
      541 gaagggctta acgtcatgct ggtaggcgac tcgctgggca tgaccgttca gggccacgat
      601 tccactctgc cagtaaccgt tgctgatatc gcctaccaca ctgccgccgt acgtcgcggc
      661 gcaccaaact gcctgctatt ggctgacctg ccgtttatgg cgtatgccac gccggaacaa
      721 gcctttgaaa acgccgcaac ggttatgcgt gccggtgcca atatggtcaa aattgagggc
      781 ggtgagtggc tggtcgaaac cgtaaaaatg ctgaccgaac gtgccgttcc tgtatgtggt
      841 cacttaggtt taaccccaca gtcagtgaat attttcggtg gctacaaagt acaggggcgc
      901 ggcaatgaag cgagcgatcg actactcagc gatgcattag ccttagaagc cgcgggggca
      961 cagctgctgg tgctggaatg cgtgccggtt gagctggcaa aacgtattac cgaagcactg
     1021 gcgatcccgg ttattggtat tggtgcaggc aacgtcactg acgggcaaat cctcgtgatg
     1081 cacgacgcct ttggtattac cggcggtcac attcctaaat tcgctaaaaa tttcctcgcc
     1141 gaaacgggcg acatccgcgc ggctgtgcgg cagtatatgg ctgaagtgga gtccggcgtt
     1201 tatccgggcg aagaacacag tttccattaa ggagtcacgt tgtgttaatt atcgaaaccc
     1261 tgccgctgct gcgtcagcaa attcgccgcc tgcgtatgga aggcaagcgc gtggcgctgg
     1321 tgcctactat gggtaatctg cacaatggcc atatgaagct ggtcgacgaa gccaaagccc
     1381 gcgccgatgt ggtcgtcgtc agtattttcg ttaacccgat gcagttcgac cgcccggaag
     1441 atctggcacg ttatccacgg accttgcagg aggactgcga gaagctaaac aaacgtaaag
     1501 tggatttagt tttcgcccct tcggtaaaag agatctaccc gaacggtact gaaacccaca
     1561 cttacgttga cgttcctggc ctttcgacca tgctggaagg tgccagccgt ccgggacatt
     1621 ttcgcggcgt ttcgactatt gtcagcaagc tgttcaacct ggtccagccg gacatcgcct
     1681 gcttcggtga aaaagatttt cagcaactgg cgttgatccg caaaatggtt gccgatatgg
     1741 gcttcgatat tgagattgtc ggtgtgccaa ttatgcgcgc caaagacggt ctggcgctaa
     1801 gttcccgtaa cggttatctg acggcggaac aacgcaaaat tgcgcctggt ctgtacaaag
     1861 ttttaagttc gattgctgac aaattgcagg ctggggaacg ggatctcgat gaaattatta
     1921 ccattgcggg gcaagaactg aatgaaaaag gcttccgcgc cgatgatatt cagattcgcg
     1981 atgccgacac attgctggaa gtttctgaaa ccagcaaacg ggcagtaatt ctggtagccg
     2041 cctggcttgg cgatgctcgc ctgatcgaca acaaaatggt cgagctggcg taatacttaa
     2101 ctggcgctac ggctgatggc gccagttatt aatttacccc acgtcatctg tcataacaca
     2161 ggtaacttaa ataaggttaa ttaccttatc aatctcagca agaggtaaat ttgccatctc
     2221 tgcaacgtct tcccgagaca ttcctttact tagaagacgc agggcgaatt cttgcctttc
     2281 ctcctgtctt ccctgctgaa tccccttctc aatccctttc tcttcaaacc actgcgccag
     2341 cgtcatcata gacttccctc ccgtttccct gtctctcagc acaccataaa acaaatccgc
     2401 ttgttcagta tgaccgcgtt gcagcatata gttttgcatg gcaactaact gacttccgct
     2461 agtgtaccct tcgtcaatca gcgtgaccag ttgctccagc aataacatta agtcgcgctg
     2521 gcgaatatgt ttttgcagta gttcgagaat cgcaatccgc cgatgttgca tgatttcgtc
     2581 atccggtgtg atggtgatat ccaccagcgg gaaaggactg ttatagacgc gtcgcgccag
     2641 ctccggcgag taaaacatat caaaccagca cattgagagc ggataaggtg tggcctcgcc
     2701 ctgataaaac agtatcggca ccaccagcgg cagcttatcg tggccagcct ccagatgccg
     2761 gtgcatggca gcaatagaat aacgcatcat gcgaaaggcc attttcttat ccggcttgct
     2821 ttggtgttca atcacaacat gcagataacc gggattgccc tgcatttgca tggaatagag
     2881 cacgtccgtg ctgtgacctt tcaggcactc ttcaatgaaa ctccccgact ctaaatgaag
     2941 cgtgttgaga tcacaaagtt cgcgtaattc cactggcaaa tgtatctcca gaaagtcgcg
     3001 agccgtctcc gcatgcatta aaaattgttt aaataccgcg tcatgcggtg tggtactcgg
     3061 tgcatccatc ttccctgtct tccgtgtggc cttgcgtgaa ggcagtatcc agtcaaggcg
     3121 tgctatcgtc agccacctct ttattgttta ccgagcagcg ttcataaaaa tatcaattcg
     3181 ttacaggcgt tacatggcac gcttcggcgc ggtacgcaaa ctgctgacgc aaatgccatc
     3241 agatgagtag acactcatca aaaatcgggc aatactgcgt gagaatttcc tcaaagcagg
     3301 caccgcctgc ttcgttaacg acagggtaga aaggtagaag ttatgattcg cacgatgctg
     3361 cagggcaaac tccaccgcgt gaaagtgact catgcggacc tgcactatga aggttcttgc
     3421 gccattgacc aggattttct tgacgcagcc ggtattctcg aaaacgaagc catcgatatc
     3481 tggaatgtca ccaacggcaa gcgtttctcc acttatgcca tcgcggcaga acgcggttcg
     3541 agaattattt ctgttaacgg tgcggcggcc cactgcgcca gcgtcggcga tattgtcatc
     3601 atcgccagct tcgttaccat gccagatgaa gaagctcgca cctggcgacc caatgtcgcc
     3661 tattttgaag gcgacaatga aatgaaacgt accgcgaaag cgattccggt acaggttgct
     3721 tgattgttat ccctgcggct ggttactcac cagccgcgac atcttctcca gcgaatccgt
     3781 tcttaagata taaagtcgtt ttagtaacaa cggattatcc cccggtttta ctttgccttt
     3841 catggttgtc accgccaggt gaaatccggc atcgtttgct gccttcacgg ctttgtcatt
     3901 aaagccgcca aacggatacg aaagatacca gacatgtgga ttaaattgcg ccagagcgcg
     3961 gcgtgaacgt gcaaaatcaa acagaatatt gtgctcacta cggctcagta atatgggtcg
     4021 gcgataacca tctacccgat gcaaaaaatg ggtatgtgac tggaaatcaa aaacatcgcg
     4081 aatttcgtta agctcagaaa cgctcataaa ttgcagcgat tttgggttcc acttctgcgg
     4141 gtgacgtttg atgcgtgagg taacaataaa caccgtcgcc ttcatgccat attgtttcaa
     4201 cacaggatac gcatagcggc tcaccgactt gaggccatca tcaaaggtaa tcaccaccgc
     4261 tcgcgcaggg agattgatcg tattcttcac gtaaccttcc agctgagcca tgctcagtgt
     4321 cgcgtatccc ctgtcgcgca gccaggccat ttggttattg aaagcgcgta ccgaggtggt
     4381 cgtcgaagta tggcgaaaac gggtgttttc ttcgtcgcgc agaatatggt gataagttag
     4441 caccggcagg ccattatcgg gttgggcatc cagcgcgctg atataggcca gtcgatcgcc
     4501 aatacggatc tgataccagg tttgatttaa cctgtctttc agtttatgca aaatcgggta
     4561 gcgcaaattg tccgccagta ccccaaatgg cgcactgccc acactcggcg cgttgtagac
     4621 cggcgtatct ttccaggtaa ttaagttctg attactcagc ggcttgttga ggtcacccaa
     4681 accgtcttca actttttgtc gcccctgaac tggctcgaga tgccctttat cgataaaccc
     4741 tttaccaaag ccaaaattaa atgcgtaata acttgcggca gtgggctcca cggcaatgat
     4801 ttgtccggcc cgaatattcc ccacggtcac catcttgtca ctaatttgcg cccagaccgc
     4861 agcattttcg atggtttgca tataacgggc aggtaacgcg gcactgacac ttgcggtaaa
     4921 cagcatcagc aggagaataa cagcttgttt atacatgaaa aaataaccga gggtgaaaca
     4981 acgcttttat tgtagcaaaa gcgccatcag aatcgcgggt taatactcat acaaattatg
     5041 tttgaggacg aaaggcggat ttttttgttg ttggtgccaa agactactca cctccggccc
     5101 acccagttcg acaatacact cacgaaactc tgaactggtc atggtttcac ggcaagccat
     5161 catctgttca attaacggta acgtgacacc agaaatcact tcgcaacggg agtgtttgtg
     5221 gcttaataat gaagccacgc gatacggtgg cgctccggct atatcggtta agaagatgac
     5281 accctcacca gagtccgctt catgcagagc atcgcacatc atgcggctga gcatattaga
     5341 gcttaatccg cgccagaaat tcacggcccg gcactgaaga agtgccccat gttttttctc
     5401 cagcgcatcc agtatctctt gcgcccggtc atcgtgacag gtaattaccc aacctaacat
     5461 cgccttcctc cttagccgaa aatcagccta ccctgaccat tgttgataaa gggtgatagc
     5521 cgtcaaaact cctctccccc gaatccagag gagaggaaat agccttagct acgcaaacca
     5581 cgtccacgtt ggatcagcga ccaacagatc aaataaaacg ccacaataaa gaccaccagt
     5641 acgccaaagg tagtgaccag cggcacatca tttataccga ggaagccgta gcggaaacca
     5701 ctgatcatat aaacgattgg gttcaggtgc gacagccctt gccagaacgg cggcaacaaa
     5761 gtcagtgagt aaaagacccc gcccaaatac gtgagtggcg ttaacacaaa ggttggcacc
     5821 aggctgatgt catcgaacgt tttggcaaac acgccgttca gcaaacccgc aagggagaat
     5881 agcaccgccg tgagcaccaa ggttaaggca acgaataccc acgaatgcac ctgaaacggc
     5941 acaaaaaaca gtgaaattgc cgtcaccaga atgccaacaa acagaccacg cgccacgcca
     6001 ccgccgacat atccggcaat aatgacgtga gtcggaaccg gcgctaccag cagctcttca
     6061 atattacgct ggaacttggc accaaaaaat gatgacgcga cgttagcgta ggcgttggtg
     6121 atcaccgaca tcatgatcag ccccggtacg atgaactgca tatagctgaa gccatgcata
     6181 tcgccaatac gcgaacctat caggttaccg aagataataa agtaaagggt catggtgatg
     6241 actggcggca ccagcgtctg cacccagata cgcataaagc gatggatctc tttcgcccag
     6301 atgcttttta gcgccaccca gtaaagatgc atcatgcgcg atctccttgt ttttcattaa
     6361 ccagtgaaac aaacagctct tccagacggt tagctttgtt acgcatactt aatacctgaa
     6421 tgccctgctc acttaactgc gtaaatacgc tgttgatccc ctgctcacgt agcacttcaa
     6481 cttccagcgt cgcggtatcg accagtcgat actgatagcc atcgagcttc ggtaacggac
     6541 ttttcggtgc gagatcgaga ataaaggttt ccgatttcag cttcgccagc agcgccttca
     6601 tcgaggtatt ttccaccagc tcaccatgtt gaataatgcc gatattgcgg cacagcattt
     6661 ctgcttcttc caggtagtgt gtggtaagaa tgatggtggt gcctttgtcg tttaaatcct
     6721 tcaaaaagcc ccacattgag cggcgaagtt caatatccac gcctgcggtc ggttcgtcga
     6781 gaatcagtag tttaggttca tgcattaacg cacgggcaat cattaaacgg cgcttcatcc
     6841 cgccagataa catacgcgca cgttcgttgc gttttcccca taaatcgagc tgcttaagat
     6901 atttttcgct gcggatgtac gcctctttgt gctccacacc gtagtaccct gcctgattca
     6961 ccacaatttg ctgaacggtt tcaaacgggt tgaagttaaa ttcctgcggc accagtccca
     7021 actgacgttt agcgttcacg acatctttct cgagatcgta accaaatacg ctgacccgcc
     7081 cggaggtttt atttaccaga gagctgataa taccgatagc ggtcgatttc ccggccccgt
     7141 tcggcccgag aagcgcataa aaatcacccg cttcgacctg caaatctatt ccacgaagcg
     7201 cctgaacgcc gcctggatag ttttttttaa gctgttgaag ttccagtgca atggtcataa
     7261 atttttactt accttacgtt cttacacttt atctgtggtt taaatcgtcc cggagttgcc
     7321 ctatattagc caaacgtaat tatttggtta caggtcgtta acctccatga aagacataga
     7381 tacactcatc agcaacaatg cactatggtc aaaaatgctg gtggaagagg atcccgggtt
     7441 ttttgagaaa ctggcacaag cgcaaaaacc gcgctttcta tggattggat gttccgacag
     7501 tcgcgttcct gcagaacgtt taaccggtct tgagccgggc gaactctttg ttcaccgtaa
     7561 tgttgctaac ctagtcattc acaccgacct gaactgcctt tccgtggttc agtatgcagt
     7621 ggatgtactc gaagttgaac acattattat ctgtggccac tacggttgcg gcggcgtaca
     7681 agccgccgtt gaaaacccgg aactggggct tatcaacaac tggctgctgc atatccgcga
     7741 tatctggttc aaacatagct cattgctcgg cgaaatgccg caagagcgcc gtctggatac
     7801 cttgtgtgaa ctgaacgtca tggaacaggt gtataacctg ggccactcca ccattatgca
     7861 atcagcgtgg aaacgcgggc agaaagttac cattcacggc tgggcctacg gcattcacga
     7921 cggcttgctg cgtgatctgg atgttaccgc caccaaccgc gaaacccttg agcaacgtta
     7981 ccgtcacggg atttccaacc tcaagctgaa acacgccaac cacaaataaa aatgccatgc
     8041 cggatgcaac acatccggca acttcacact tactcgtcca gcagaatcac tttgccaata
     8101 tacggcagat gacggtaacg ctgtgcgtaa tcaatgccgt aacccaccac aaactcatcc
     8161 gggatcgaga aaccgataaa ttcaaccggg acgttcactt cacgacggga cggtttatcc
     8221 agcagcgtac aaatcgccaa cgacttcggt tcgcgcaggc ttaagatctc acgcactttc
     8281 gacagtgtat tccccgagtc gatgatatct tcaacaatca gcacgtcctt gccacggata
     8341 tcttcatcca gatctttgag gattttcaca tcacgggtgg tggacatgcc gctaccgtag
     8401 ctggaggcgg tcataaagtc gacttcatga gatacctgaa cttcacggca caggtccgcc
     8461 ataaacataa atgagccacg cagcagaccc accagcacca tatcgctgcc gctgtctttg
     8521 taacgctcag taatctgacg acccagttcg gcgatacgcg ctttaatctc cgcttcgggg
     8581 atcattactt ctacagtatg tttcatatct ctaaccatat gatttaaaaa caaatcactt
     8641 cgcacgggca ggttttggca ccgcaacaaa gcgcaagacg cgcagtatac cagtaaaact
     8701 attcattcgc ggaatctgtt aataaagctc gaattgtgat gacggtcaca catgttaaaa
     8761 cctataatta attgctacta aaatattaat gaattgaaat ggtgtctctt tatggcaatt
     8821 aacaatacag cctcgcgacg attactcgtc acgctaacag ccctttttgc agcgctttgc
     8881 gggctgtatc tactcattgg cggaggctgg ctggtcgcga ttggcggctc ctggtactac
     8941 cctatcgctg gccttgtgat gctcggcgtc gcctggatgc tgtggcgcag taaacgcgcc
     9001 gcgctttggc tatacgcagc cctgctgctc ggcaccatga tttggggcgt ctgggaagtt
     9061 ggtttcgact tctgggcgct gactccgcgc agcgatattc tggtcttctt cggcatctgg
     9121 ctgatcctgc cgtttgtctg gcgtcgcctg gtcattcctg ccagcggcgc agttgccgca
     9181 ctggtggtcg cactgctgat tagcggtggt atcctgacct gggccggatt taacgatccg
     9241 caggagatca acggcacctt aagcgccgat gccacacctg ccgaagctat ctcccccgtt
     9301 gccgatcagg actggcctgc gtatggccgc aatcaggaag gtcaacgctt ttcaccactg
     9361 aagcaaattc acgccgataa cgtccacaag ctgaaagaag cctgggtgtt ccgtactggc
     9421 gatgtgaagc agccgaacga tccgggtgaa atcaccaatg aagtgacgcc gattaaagtg
     9481 ggcgataccc tttacctgtg taccgctcac cagcgcctgt ttgcgcttga tgccgccagc
     9541 ggcaaagaga aatggcatta cgatcctgag ctgaaaacca acgagtcttt ccagcacgta
     9601 acctgccgtg gcgtctctta tcatgaagcc aaagcagaaa cggcttcgcc ggaagtgatg
     9661 gcggattgcc cgcgtcgtat cattcttcca gtgaatgatg gtcgcctgat tgcgattaac
     9721 gctgaaaacg gcaaactgtg cgaaaccttc gccaataaag gcgtgctcaa tctgcaaagc
     9781 aatatgccag acaccaaacc gggtctgtat gagccgactt ctccgccaat tatcaccgat
     9841 aaaaccatcg tgatggccgg ttcagtcacc gataacttct caacccgcga aacgtctggc
     9901 gtgatccgtg gttttgatgt caacaccggg gagctgctgt gggcttttga tccgggcgcg
     9961 aaagatccga acgcaatccc gtcagacgaa cacaccttta cctttaactc accaaactcg
    10021 tgggcaccag cggcctatga cgcgaagctg gatctggtct atctgccgat gggcgtgacc
    10081 acgccggata tctggggcgg taaccgcaca ccggaacagg aacgttatgc cagctcgatt
    10141 ctggcgctga atgccactac cgggaaactg gcgtggagct accagaccgt tcaccacgac
    10201 ctgtgggaca tggatcttcc ggcacagccg acgctggcgg acatcaccgt taatggtcag
    10261 aaagtgccga ttatttacgc tccggcgaaa accggtaaca tttttgtgct cgatcgtcgt
    10321 aatggcgaac tggtggttcc ggccccggaa aaaccggttc cccaaggtgc tgccaaaggc
    10381 gattacgtta cccctactca accattctcg gaactgagct tccgtccgac gaaagatttg
    10441 agcggtgcgg atatgtgggg agccaccatg tttgaccaac tggtgtgccg cgtgatgttc
    10501 caccagatgc gctatgaagg cattttcacg ccgccatctg aacagggtac gctggtcttc
    10561 ccgggtaacc tggggatgtt cgaatggggc gggatttccg ttgatccaaa tcgtgaagtg
    10621 gcaattgcca acccaatggc actgccgttt gtttcgaaac tgctcccgcg tggtcctggc
    10681 aacccgatgg agcagccgaa agatgccaaa ggcacgggta cggaatccgg cattcagcca
    10741 cagtacggtg taccgtatgg tgtcacgctc aacccgttcc tctcaccatt tggtctgcca
    10801 tgtaaacagc cagcatgggg ttatatctcg gcgctggatc tgaaaactaa tgaagtggtg
    10861 tggaagaaac gtattggtac gccgcaggac agtatgccgt tcccgatgcc ggttccggtg
    10921 ccgttcaata tgggtatgcc gatgctgggc gggccaatct ccacggcggg taacgtgctg
    10981 tttatcgccg ctacggcaga taactacctg cgcgcttaca acatgagcaa cggtgaaaaa
    11041 ctgtggcagg gtcgtttacc ggcgggtggt caggcaacac cgatgaccta tgaagtgaat
    11101 ggcaagcagt acgtggtgat ctccgcaggc ggtcacggtt catttggtac gaagatgggc
    11161 gactatattg tggcttatgc cctgccggat gatgtgaaat aaccataatg cccggagaga
    11221 tccgggcata tttccgaata cggtcttttt ataccgtaaa ccctaacatc atccccgtat
    11281 cttcatgctc cagcagatgg cagtgcgcca tataagcatg ttctttcggt gcgttgtgat
    11341 taaacttcac cagcacttca ctgacattgc cttctacctt aacggtatct ttccagcccg
    11401 cgcgatgagc cgctggcggt ttgccatttt ctgacaagat acggaactgc gtgccgtgaa
    11461 tatggaacgg atgcagcatc atgtcgccca cgccagagat aacccaacgt tcgtactgcc
    11521 ctttcgccgc cgcaaacatc ggcttattca tatcaaacgc ctgaccgttg attttattgg
    11581 catggtggaa atcgaacttc ccgccgtggt tcatatgatt catattgccg tgccccatat
    11641 ggcccatcat ctggctgtga tccattccgg ccatcgcctg atcgccatat ttctccatta
    11701 gcatctgcat ccccatcata tcgagcatcg ggtccataga aagttgcagc ttgcgtaccg
    11761 tcagcccttc cagcgaaggt aacgcaggca ggctacttaa tgtgtcaggc aaagcaccgg
    11821 aggcactaat agcaatcggc tgaatccgca ttaccggatg cggcttgtca aacggtgcaa
    11881 tcgccatccc catctggctg accggcagcg tcaccaggtc aaagggtttg ttgtcgttaa
    11941 cctccaccag cacttcaaaa cgctcgccca tcagcaccgg cagctcgctc accttcaccg
    12001 gttcaggtag cagaccaccg tcgctggcaa tcacatacag cgggcgattg tcgctggtgg
    12061 cgaaattgag cgagcgggca ttacagccat tgagcaaacg caggcgcagc caaccacgcg
    12121 gggcagcgtg ttgtgggtag attgcaccgt tggtcagcag cgtatcgcca aaccagccca
    12181 cggcggcggt catcacatcc agttgataat caatctgccc gtcggcgcta aatttcttat
    12241 cctgaacgat caccggaaca tcatcgatac cccactgttt tggcagcatt aatttcagga
    12301 tctcgtcatc ttcaatcacc accagaccag ccagccccat cgccacctgt cgcccggtct
    12361 tgccatgttg atgtggatgg aaccagcagg tagcggcagg ttgatcaacg ttcaacgtca
    12421 ccgagcgctt gccacctggc ggaataattc cctgtgggcc gccgtcgacg tcacccggta
    12481 cttccagccc gtgccagtgc aacgtcgtct cttccgccag ttggttgtag atatcaaccg
    12541 ttaccgcttt gccgcgctgt aatttcaccg ccggccccag cagattgccg ttatagcccc
    12601 aggtagttgc agttttctcg ccaaaggtgg actgacctgc gccaatagtt aactgaatgc
    12661 gattacgggc atcggtcgtg agcaaatcag ggatcggtaa cgttgggcgt tccgccgcaa
    12721 agactgcacg gctccacagt ggcaaggctg aagccacacc cagtgcgacg gaatatttca
    12781 agaaatcacg acgttgcata gttatttcct tattcttaag caggcgaaac aaaatcaaac
    12841 gttgagcata gtccttcccc ttacgggaag gtcaagccgc agacacaatt taatcgacaa
    12901 taatggtcgt cgcctcgctc gcagtgtgct aacgtttatc ttctttaagc ccctggtaga
    12961 agcaatgaag acgtttttca gaacagtgtt attcggcagc ctgatcgccg tttgcgcaaa
    13021 cagttacgcg ctcagcgagt ctgaagccga agatatggcc gatttaacgg cagtttttgt
    13081 ctttctgaag aacgattgtg gttaccagaa cttatctaac gggcaaattc gtcgcgcact
    13141 ggtctttttc gcccagcaaa accagtggga tctcagtaat tacgacacct tcgacatgaa
    13201 agctctcggt gaagacagct accgcgatct cagcggcatt ggcattcccg tcgctaaaaa
    13261 atgcaaagcc ctggcccgcg attccttaag cctgcttgcc tacgtcaaat aatccctgat
    13321 acctttttgt agaaatattg accgtgcatc cgcggtcaat gttagctatt atgttgcgcc
    13381 ctttttttac gggtgttaac aaaggaggta tcaacccatg gccgaaaaaa aacagtggca
    13441 tgaaacgcta cacgaccagt ttgggcagta ctttgcggta gataacgttc tgtatcatga
    13501 aaagaccgat caccaggatc tgatcatttt tgagaacgct gcatttggtc gcgtaatggc
    13561 gctggatggc gtagtacaaa ccaccgagcg cgacgagttt atctatcatg agatgatgac
    13621 ccatgttccg ctactggccc acggtcacgc gaaacatgtg ctgattatcg gcggcggcga
    13681 cggtgccatg ctgcgtgaag taacccgaca taaaaacgtt gagtcaatca cgatggtgga
    13741 aatcgatgcg ggtgtcgtgt cgttctgccg tcagtatctg cccaaccata acgccggtag
    13801 ctacgacgat ccgcgctttc aactagtgat cgacgatggc gtcaatttcg ttaatcaaac
    13861 cagccagacc tttgatgtca ttatctccga ctgcaccgat cctatcggtc ccggcgaaag
    13921 cctgttcacc tcagcatttt atgaaggctg caaacgttgc ctgaatcctg gcggtatctt
    13981 cgtcgcacaa aacggcgtct gctttttaca gcaggaagaa gccatcgaca gccatcgcaa
    14041 actcagccat tacttcagcg acgttggctt ttatcaggcg gcgatcccga cctattacgg
    14101 cggtatcatg acttttgcat gggcgacaga taacgacgcc ttacgccatc tctcaaccga
    14161 aattattcag gcgcgttttc tcgcttctgg cctgaaatgc cgttattaca atccggcaat
    14221 ccatacggca gcttttgcct tacctcagta tctgcaagac gcactggctt cacagccgtc
    14281 ctaaggagaa gataagaaat tgaaaaaact gaaactgcat ggctttaata atctgaccaa
    14341 aagtctgagt ttttgtattt acgatatctg ctacgccaaa actgccgaag agcgcgacgg
    14401 ttatattgct tatatcgatg aactctataa tgccaaccgt ctgaccgaaa tcctgtcaga
    14461 aacctgttcc attatcgggg ccaatattct taacatcgcc cgccaggatt acgaaccaca
    14521 gggtgccagc gtcactattc tggtgagtga agaaccggtt gacccgaaac tcatcgacaa
    14581 aacagaacac cccggcccac tgccagaaac ggtggttgct catctcgata aaagtcatat
    14641 ttgcgtacat acctacccgg aaagtcatcc tgaaggcggt ttatgtacct tccgcgccga
    14701 tattgaagtc tctacctgcg gcgtgatttc tccgctgaag gcgctgaatt acctgatcca
    14761 ccagcttgag tccgatatcg taaccattga ttatcgcgtg cgcggtttta ctcgcgacat
    14821 taacggtatg aagcacttta tcgaccatga gattaattcg attcagaact ttatgtctga
    14881 cgatatgaag gcgctgtatg acatggtgga tgtaaacgtc tatcaggaaa atatcttcca
    14941 taccaagatg ttgcttaaag agttcgacct taagcactac atgttccaca ccaaaccgga
    15001 agatttaacc gacagcgagc gccaggaaat taccgctgcg ctgtggaaag aaatgcgcga
    15061 gatttattac gggcgcaata tgccagctgt ttaacggctc tggcggagct cccaggctcc
    15121 gccagattta tttacttctg ctgcacgaaa ttgcggtaag ccgccacgac ctgcagaaaa
    15181 tcctcaacgc cgcatagcga caggctttct tcgtcgtagt agttcatccc ctcttccatt
    15241 tcatcgccag cgaattccag ttgattggcg cgaaccatca cctcttcagc gtccatccac
    15301 agggtgtatt catgccctgc ccgttgccag gaccgttcgc tacctttcag tgcgtgcgct
    15361 gcttgttcca cttcatcaag caaggccagg ttttctttca cctcttcatt aaaccagtgc
    15421 ccgaccactt catgccccat ggacatacgc acctttacca ctccggtaat atcgcgcaga
    15481 aattcgtaat ccatagtgtg ttcctctgct cccggcaatg cgccgaaatc gcatcgcgcc
    15541 attgatgtaa ttatcgcagc cttaccggag aaaaacagcg gaacggcgat agtttagaaa
    15601 taaaaaatgc ccgggaatct cattcaccgg gcattgtgtc gtttatgcgc agcgcgtgcg
    15661 cttacttatt acaccgcagt ctggaaaatc accccatcgg ctttttcggt gtactgagaa
    15721 agctggttga agttcagata acgataagta tcaacggcag ttttatctac ttgcgcaacg
    15781 taggtctggt actcttccgg cgtcggcagt ttgccaatca gcgccgcaac agccgccagt
    15841 tctgcagaag ccaggaagac attcgcgcca gtacccaaac ggttcgggaa gttacgggta
    15901 gaggtggaaa ccaccgtcgc accgtctgct acacgcgcct ggttacccat acacagggaa
    15961 cagcccggga tctcgatacg agccccgctc ttaccgaaga cgctgtagta gccttcttcc
    16021 gtcagttgcg cggcgtccat acgggttggc ggtgccaccc acaggcgggt cggcaactga
    16081 cctttgtgcg catccagcag tttaccagcg gcacggaagt gaccgatgtt agtcatgcaa
    16141 gaaccgataa atacttcgtc gatcttctcg ccctgtaccg cagacagcgg acgtgcgtca
    16201 tccggatcgt tcggagcaca caggattggc tctttaatct ccgccagatc gatgtcgatc
    16261 actgccgcgt attccgcatc tgcatcggct tccagcagct caggattcgc cagccatttt
    16321 tccatgccct gaatacggcg ttccagggta cgacgatcgc cgtaaccttc cgcgatcatc
    16381 cacttcagca ggacgatgtt ggagctcagg tattcgatga tcggttcttt gttcagcttg
    16441 atagtacaac cagcggcaga acgctcggcg gacgcatcgg ttagctcaaa ggcctgctca
    16501 actttcagat ccggcagacc ttcaatttcc agaatgcgac cagagaagat gtttttcttg
    16561 cctttcttct caacggtcag cagaccttgt ttgatcgcat acagcggaat ggcgtgtacc
    16621 agatcgcgca gggtgatgcc cggctgcatt ttgcctttga agcgcaccag aacggattcc
    16681 ggcatatcca gcggcattac gccagttgcg gcagcaaacg ccaccagacc agaacccgcc
    16741 gggaaagaga taccgatcgg gaaacgggta tgggagtcac caccggtacc gacggtatcc
    16801 ggcagcagca tacggttcag ccaggagtga atgacgccgt cacccggacg cagcgacaca
    16861 ccgccacggt tcataatgaa gtccggcagc gtgtggtgcg tgttcacgtc aactggcttc
    16921 ggatacgccg cggtgtggca gaaagactgc atcaccaggt cagccgagaa gcccaggcac
    16981 gccaggtctt tcagttcatc acgggtcatc gggccggtgg tgtcctgaga gcctacagaa
    17041 gtcattttcg gttcgcagta cgcgcccgga cgaatgcctt tcacgccaca ggcacggcct
    17101 accattttct gcgccagcga gaagccgcga tcgctctcag cgacatcttt cgcctgacgg
    17161 aacacatcac tgtgcggcag accaagtgct tcacgcgctt tggtggtcag gccacgcccg
    17221 ataatcagcg gaatacggcc gccagcacgc acttcatcaa tcagcacgtc ggttttcagt
    17281 tcgaaggtcg ccagcagttc gcctgtttcg tggttacgca cttcaccttt gtacgggtaa
    17341 acgtcaatca cgtcgcccat gttcaggtta gagacgttga cttcgattgg cagtgcaccc
    17401 gcgtcttcca tcgtgttaaa gaagatgggt gcaattttac cgccgaggca caaaccaccg
    17461 ccgcgtttgt tcggcacatg tggaatatca tcgcccataa accacagaac ggagttcgtg
    17521 gcggatttac gcgatgaacc cgtacccaca acgtcaccga cgtacgccag cgggaaacct
    17581 ttctgttgca gagcttcaat ctgtttgatc gggccaacaa cacctggctg gtctggctca
    17641 ataccttcac gggcgttttt cagcatcgcc agcgcgtgca gtgggatatc cgggcgtgac
    17701 cacgcatccg gtgccggaga aaggtcatcg gtgttggttt cgccagtgac tttaaagacg
    17761 gtaacggtca gtttttcagc cagcgccggg cgattcagga accattcggc atccgcccag
    17821 gactgcatta cctgcttcgc atattcgttg cctgctttcg ctttctcttc tacgtcatag
    17881 aagttatcga acatcagcag cgtgtgagaa agtgctttgg cagcaatagg tgccagtttg
    17941 gcatcatcca gcgcgtcgat cagcggatga atgttgtaac caccctgcat ggtgcccagc
    18001 agttcgatag ctttttccgg agtcagcaga ggggatttag cttcgccttt agcgatagca
    18061 gccaggaagc ctgcttttac ataggcggct tcatcgacgc ctgggggaac acggttggtt
    18121 aacagatcta acaggaattc ttcttcgccc gcgggcgggt ttttcagcag ctctacaagc
    18181 gcggccattt ggtttgcatc caggggtttg ggcgcaatcc cctcagcggc acgctcagct
    18241 acgtgcttac ggtattcttc tagcacgacg gttctcctcg ctctcattgt catagtgcgg
    18301 caggcgattc tcttcacgct cctgtgagac agcagtttgt agggtaaatg cccagtaccg
    18361 caacgggcag aatagcagga ttttgacgac gtgttaatct gtttacaaaa aagcaacata
    18421 aaaaagtggc tgaatcgtta aggatggtct agagattgtt tccctccgta aaaacttcgt
    18481 taattcgcat ggtgataatc acctttctca acgaatccca agattagtca aaatttaaac
    18541 taccgcctct ttatactcgg attcacagca cctgcgggtg gcagttcgcc gaccattgcg
    18601 atttccttga gatccgaatt atgaaaatga ctttgccgtt taaaccccat gtgctggcgc
    18661 taatttgcag tgccgggctt tgtgctgcct ctgccgggct atatataaaa agccgcacag
    18721 tggaagcgcc tgtagaaccg caatcgacac aactggctgt gtctgacgct gccgctgtta
    18781 cgcttcctgc aacggtttcc gcacctcccg taatacccgc agtcgtcaaa tccgcattca
    18841 gcactgcaca aatagatcaa tgggtcgcac ccgtcgcgct gtatcccgac gccctacttt
    18901 cgcaggtgct gatggcatca acctatccga caaacgttgc tcaagcagtg caatggtcgc
    18961 acgataatcc acttaaacaa ggcgatgctg ctattcaggc ggtatctgac cagccgtggg
    19021 acgccagcgt taaatcactg gtggcctttc cacaattgat ggcattgatg ggcgaaaacc
    19081 cgcaatgggt gcaaaacctg ggcgatgctt ttctggcgca gccgcaggac gtgatggact
    19141 cggtacagcg attgcggcaa ctggcgcaac aaaccggctc gctgaagtca tcaaccgaac
    19201 agaaagttat taccacaacg aagaaagctg taccggtaaa acagacagtc acggcacccg
    19261 tcataccatc caataccgtt ttaactgcca accccgtcat tacagagccg gcaacaaccg
    19321 tcatttccat tgagcccgcc aatcctgatg tggtctatat tcccaactac aacccaaccg
    19381 tggtttacgg gaactgggcc aatactgcgt atccgccggt ttatctgcca ccaccagccg
    19441 gagaaccgtt tgttgacagc tttgtgcgcg gattcggcta tagcatgggc gttgctacca
    19501 cgtacgcact attcagcagc atcgactgag atgacgacga tcatgaccat catcatcatg
    19561 acgatgatga ttatcatcac cacgatggcg gtcatcgtga cagtaatggc tggcaacaca
    19621 acggcgacaa catcaatatc gacgtcaaca atttcaaccg tatcaccggt gagcatctta
    19681 ctgataagaa tatggcatgg cggcacaatc caaactaccg taatggtgtg ccctatcatg
    19741 atcaggatat ggcaaagcgg tttcatcaaa ccgatgtcaa cggcggaatg agtgccacgc
    19801 agctacctgc tccaacacgc gacagccagc gtcaggcggc agcaagtcag tttcagcaac
    19861 gaacacacgc cgcccccgtc attacacgag atacccaacg tcaggcagcg gcacagcggt
    19921 ttaatgaagc tgaacactat gggagctatg acgacttccg cgacttcagc cgtcgccaac
    19981 cactgaccca gcagcaaaag gacgccgctc gtcagcgtta tcagtcggcc tctcctgagc
    20041 agcgccaggc agtccgcgag aaaatgcaga ctaacccgca gatccagcag cgaagggagg
    20101 cggcgcgcca gcgtattcag tccgcctcgc ctgagcagcg ccaggcggtt cgcgagaaaa
    20161 tgcagactaa cccgcagaac cagcagcgaa gagaggcagc gcgtgagcgt attcagtctg
    20221 caactcccga acagcgccag gtgtttaagg aaaaagtaca gcagcgccca ctgaaccaac
    20281 agcaacgtga taacgcccgc cagcgtatac aatcggcatc acctgaacaa cgtcaggttt
    20341 ttcgggagaa agttcaggag agccgcccac aacgtctaaa cgacagtaac catactgtca
    20401 ggctgaataa cgagcaacgg tcagcagtac gcgaacgtct ctctgaacgt ggagcaagaa
    20461 gactggaaag gtaaattgca gacgtaaaaa aagcggcgtg gttagccgct tttttaattg
    20521 ccggatgttc cggcaaacga aaaattactt cttcttcgct ttcgggttcg gcaggtcggt
    20581 aatgctacct tcgaacactt ctgccgccag gcccacagac tcgtgcagag tcgggtgcgc
    20641 gtggatggtc agtgcgatgt cttcagcatc acaacccatt tcgattgcca ggccgatttc
    20701 acccagcagc tcgccgccgt tggtaccaac aatcgcacca ccgatcacac ggtgagattc
    20761 tttgtcgaaa atcagcttgg tcataccgtc tgcgcagtca gaagcgatag cacgaccaga
    20821 agcagcccac gggaaggtgg cggtttcata gctgatgcct ttctctttcg cttctttctc
    20881 agtcagacct acccatgcaa cttctggttc ggtataggcg atggacggga taactttcgg
    20941 atcgaagtag tgtttcttac cggcgataac ttcagcggca acgtgacctt cgtgaacacc
    21001 tttgtgtgcc agcatcggct gaccgacgat atcgccgata gcaaagatgt gcggtacgtt
    21061 ggtacgcagc tgtttgtcaa cgcggatgaa accacggtcg tcaacttcca cgccagcttt
    21121 gcctgcgtcg aggtttttac cgttcggcac acgaccaatc gctaccagca cggcgtcgta
    21181 acgctgcggt tcagctggtg cttttttgcc ttccatcgtc acataaatac cgtcttcttt
    21241 cgcttcaacg gcggtaactt tggtttccag catcaggttg aatttcttgc tgatacgctt
    21301 ggtgaagact ttaacgatgt ctttgtcagc tgccgggata acctggtcga acatttcaac
    21361 cacgtcaatc tgtgaaccca gcgcgtggta aacggtgccc atttccagac cgatgatacc
    21421 gccacccatt accagcaggc gttctggtac ttctttcagt tccagcgcgt cagtggagtc
    21481 ccagatacgc ggatcttcat gcggaataaa cggcagttgg atcgggcgag aacccgctgc
    21541 aatgatcgca ttgtcgaagt tgatcacggt tttgccgttc tcaccttcaa cttccagggt
    21601 gttagccccg gtgaatttac ccagaccgtt gaccactttg actttgcggc ctttcgccat
    21661 accagccaga ccaccggtca gctgattgat cactttctct ttccaggtac gaatcttgtc
    21721 aatatcggtt ttcggttcgc cgaagacgat accgtgttca gccagcgctt tggcttcttc
    21781 gataactttt gctacgtgca gcagtgcttt agaagggata cagccgacgt tcaggcaaac
    21841 accgccaagg gtgttgtaac gttctacgat tacggtttcc agacctaaat cagcgcaacg
    21901 gaaggcagcg gagtaacctg cggggcctgc cccaagtacc acgacctgag ttttgatttc
    21961 agtactcatc atgacctcta tatatttatc tccggcggtc atacccgtcg tctttcaggc
    22021 cgcagcggcg ttagcttcac gccctcaccc cagtcactta cttatgtaag ctcctgggga
    22081 ttctggcgct tgctaccttg ctgcacccag aaatccatag ggtatgtgaa gtaataccct
    22141 aaccaccacc gggtcgttct atccgtcggt attttacaaa attgttaaca atttttaaac
    22201 aacaaacggc aaccgatttg tctattcgct aataacctca atacattcat gagattacca
    22261 gaaaaaagcc ggccgttggg ccggctcttt tacttacatc accagacggc gaatgtcaga
    22321 cagcgtgttg ttaatgatgg taatgaaacg ggcaccatca gcaccgtcga tcacgcggtg
    22381 gtcgaaggag agagaaatcg gcagcatcag acgcggcacg aactctttac cattccacac
    22441 cggctccatc gcggacttgg aaacgccgag gatagccact tccggcgcgt tcacaatcgg
    22501 cgcgaagtgg gtagtaccca ggccgccgat gctggagatg gtgaagcaac cgccctgcat
    22561 ttcgcccgca gtcagcttac cgtcacgcgc tttcttagaa atagtcatca gctcgcgaga
    22621 cagctcgatg atgcctttct tgttgacgtc tttgaatacc ggaacaacca gaccgttcgg
    22681 ggtatccacc gccacaccga tgttgatgta tttcttcagg gtcagacgct gaccgtcttc
    22741 cgacagcgaa ctattgaagc gaggcatctg ctcaagagct gcagcaacgg ctttcatgat
    22801 gaagacaacc ggggtgatct tcacatccag cttacgtttc gccgcttctt cgttctgctg
    22861 tttacggaac gcttccagct cggtgatatc ggttttgtcg aagtgggtaa catgcgggat
    22921 catcacccag ttacggctca ggttagcacc agaaattttc tggatacggc ccagttccac
    22981 ttcttcgatt tcgccaaact tgctgaagtc caccttcggc cacggcagca tgccagggat
    23041 accaccgcca gtcgccgccg gagctgcttc tgcacgtttg atagcttctt tcacgtaagc
    23101 ctgaacgtct tcgcgcagga tacgaccttt acggccagtg cccttcactt tcgccaggtt
    23161 aacgccaaac tcgcgtgcca gacggcggat cagcggggtc gcgtgaacgt aagcgtcgtt
    23221 ttcagcaaat tcagatttgc cttccgcttt cgcagccgga gcttcagctt ttgctgccgg
    23281 agccggcgct gccgcttcct gtttcgcagg agctgccgca ggcgctgcgc cttcaacttc
    23341 gaagatcata atcagcgaac cagttttcac tttatcgcca acgttgactt tcagttcctt
    23401 cacgacgcct gcaaacggag ccggaacttc catagaagct ttatcgcctt ctacggtaat
    23461 cagtgactgt tcagcggcaa ctttgtcgcc cactttcacc atcacttcgg tcacttcaac
    23521 ttcgtcaccg ccgatatccg gaacgttaac ttctttcacg ccagctgctg gtgcaggggt
    23581 cgctgccgga gtcgcttcct gtttagccgc cggagctgcc gcgcctgctt cacccgcgac
    23641 ttcgaagacc ataatcagcg agccggtaga cactttgtca cccacgttca ctttgatctc
    23701 tttcacggta cctgcaaacg gagccggaac ttccatagaa gccttgtcgc cttctacggt
    23761 gatcagcgac tgttcagctt caactttatc gccaactttc accaggattt cggtcacttc
    23821 aacttcgtcg ctgccgatat ccggaacgtt aacgtctttt gccgccgcag ccgcgggtgc
    23881 tgctgccgga gctgcttctt tcttctcttc tgcctgagca ggtgcagcgt ctgctgcacc
    23941 gtcggcggaa tcgaaaatca taatcagtgc gccggtctgg gttttatcgc caacagagac
    24001 tttgatctct ttaacgatac ccgcctgcgg agatggaact tccatagagg ctttgtcgcc
    24061 ttctacggtg atcagcgact gttcggcttc aactttgtcg cccactttga ccaggatctc
    24121 ggtgatttca acttcatcag ccccgatgtc cggtactttg atttcgatag ccattattct
    24181 tttacctctt acgccagacg cgggttaact ttatctgcat cgatgttgaa tttggcgatt
    24241 gcgtcagcaa ccactttctt atcgatttcg ccacgtttag ccagttcgcc cagcgccgca
    24301 accacgacat aagaagcatc aacttcgaag tggtgacgca ggttctcacg gctgtcggaa
    24361 cgaccgaagc catcagtacc cagtacgcga tagtcgtcag ccggtacgta agtacggacc
    24421 tgctcagcga acagtttcat atagtcggta gatgccactg ccggcgcgtc gttcatcacc
    24481 tgagcgatat acggaacgcg cggagtttcc agcggatgca gcatgttcca gcgttcacaa
    24541 tcctgaccat cacgcgccag ctcggtgaag gaggtcacgc tataaacgtc agaacctacg
    24601 ccgtaatctt tcgccaggat ctctgccgct tcacggacgt gacgcaggat ggaaccggag
    24661 cccagcagct gaactttacc tttgctacct tcaatggttt cgagtttgta gataccttta
    24721 cggatacctt cctcagcacc ttccggcatt gccggcatgt ggtagttttc gttcagcgta
    24781 gtgatgtagt agtaaacgtt ctcttgtttt tcaccgtaca tacgctccag accgtcatgc
    24841 ataatgacag caacttcgta agcgtaagcc gggtcgtaag agatacagtt cgggatagtc
    24901 agcgactgaa tgtggctgtg accatcttcg tgctgcagac cttcaccgtt cagggtggta
    24961 cgaccggaag taccgccgat caggaagcca cgcgcttgct ggtctccagc cgcccagcac
    25021 agatcgccga tacgctggaa gccgaacatc gagtaataga tgtagaatgg gatcatcggc
    25081 agattgttcg tgctgtaaga ggtcgccgct gccagccagg aacaccctgc gcccagctcg
    25141 ttgatccctt cctgcagaat ctgacctttc tcgtcttctt tatagtaagc aacctgctcg
    25201 cggtcctgcg gggtgtactg ctgaccgttc gggctgtaaa taccaatctg acggaacaga
    25261 ccttccatac cgaaagtacg cgcttcgtcg gcgatgatcg gtaccagacg atctttgatc
    25321 gacttgttct tcagcatcac gttcagagca cgaacgaaag cgatagtggt agagagctct
    25381 ttgctctgct cttccagcag cgcgccgaag tcttgcaggc tcggcagctc aagcttctcg
    25441 gtgaagttcg gctgacggct tggcagataa ccgtgcagtt tctgacgctg agcgtgcaga
    25501 taggtatgct cttcagaacc ttccgggaag gtgatgtacg gcagtttttc gatatctgca
    25561 tcagacaccg gcacattgaa acggtcgcgg atgtgacgca cgccgtccat gttcattttc
    25621 ttaacctggt gcgcgatgtt tttaccttca gccgcgtcgc ccatgccgta acctttaatg
    25681 gtatgagcaa ggattactgt cgctttgcct ttggtttcct gcgctttctt gaatgcagcg
    25741 tagattttct tcggatcgtg accaccacgg ttcagtgccc agatctgctc gtcagtccag
    25801 tctgcaacca gtgctgcggt ttcaggatat ttaccgaaga agtgttcacg aacgtacgca
    25861 ccatctttcg atttgaaggt ctggtagtcg ccgtcaacgg tttcgttcat cagctggatc
    25921 agtttaccgc tggtatcttt acgcagcagt tcatcccaac ggctacccca catcactttg
    25981 atcacgttcc agccagcacc ttcgaagatg ccttccagtt cgttgatgat cttgccgtta
    26041 ccggtgaccg ggccgtcaag acgctgcagg ttacagttga taacgaaaac caggttatcc
    26101 agtttttcac gggtagcgat ggtgatcgca cctttggatt ccggttcgtc catttcaccg
    26161 tcgccgagga acgcgtaaac ggtctgttta gaggtatctt tcaggccacg gtgttccaga
    26221 tatttcagga atttagcctg gtaaatagca ccaatcggac ccagacccat agatacggtc
    26281 gggaactgcc agaattccgg catcagtttc gggtgcggat aggaagagag gccattgccg
    26341 tgaacttcct gacggaagtt atccagctgc tcctgagtca gacgaccttc caggaaagca
    26401 cgggcgtaaa cgcccgggga gatgtggccc tggaagtaaa ccaggtcgcc gccatcctgc
    26461 tcgttgcgtg cacggaagaa gtggttaaag cacacatcat aaatggttgc ggaagactgg
    26521 aaggacgcca tgtggccgcc cagttcgagg tcttttttcg acgcacgcag caccgtcatg
    26581 atggcgttcc agcggatagc tgaacgaata cggcgttcca gttccagatt acccggatac
    26641 tccggttgtt cttcaacggg gatggtgttg atgtagttgc tgatacctgt gcctgcggct
    26701 acgtttacac cgcctttgcg ggcttcagca agcagttggt cgatcagata ctgagcacgc
    26761 tcaacacctt cttcacggat gaccgattcg atcgcctgga gccagtcgcg agtttcgatc
    26821 ggatccacgt catttgggaa acgttctgac atgggttatt ccttatctat ctaataacgt
    26881 tgagttttct ggaacctgtc ccattgaact ctcgccagaa agctcaataa gacaggttct
    26941 acgtttagtt accgcgcttt tatatgcgct tgatttacaa catcttctgg ataattttta
    27001 ccagaaaaat cactaattct ttcgttgctc cagacgacgc agagaacgct cacggcggct
    27061 ctcttcacgg cttctgtcga gcaaaatttc ttcgataaag gccagatggc gatgcgatgc
    27121 ttcgcgcgct tcttccggct taccggccat aatcgcttca aatatgcggg tgcggtgact
    27181 actcaccagc ggcagcatct cgcgacgcga atagagcaat tcgaagttct ggcggacatt
    27241 ctgggccaac atcggctcca tacaccttag cagatgaagc agaaccacat tgtgggccgc
    27301 ttcggtgacg gcaatctgat actggagtac ggcgtttgat tccgcgtcca gatcgccaga
    27361 ctgctgcgcc agctctatgg cgtggtggag ttcacggatg cgttccttgt cttcatcggt
    27421 actacgcagc gcggcgtaat aagcggcgat accttccagg gcgtgtcgtg tttcgagcaa
    27481 gtcatactgt gattcaggat ggtcggagag cagctccacc agcggatcgc tgaagctttg
    27541 ccataggctg ctctggacaa aagtgccgcc accctggcga cgaagcaaca agcccttcgc
    27601 ttcgagacgt tgaatcgcct cacgcaagga gggacgggag acgtcgaact gttttgccag
    27661 ttcgcgttcc ggtgggagtt tttcgcccgg gcggagagtg ccttcgagga tcaaaaactc
    27721 cagttgctgc tcaatcacat cggagagttt tggttggcgg attttgctgt aggccatgag
    27781 ttcctgtctt aagccacttg ccgaagtcaa ttggtcttac caatttcatg tctgtgacgc
    27841 taaagtaaca aagtattcac cttatgtcca tacaggtttt gattgaaatc atgaaactgt
    27901 gcacatttta acaacttgac atatataacg tttcaaagtt gtaactatgc acaaatgtag
    27961 actttacgta ggaaagggat tttttaacga ttattaaact ataaaaatcc gaattgaacc
    28021 gattcactta ccaatttttt gatttttaag tcaataaaaa cgaatttaaa ttcactctgc
    28081 atattgagag gggttgaggc tgagctttac aaacggtttc tttttaagca actcatcttc
    28141 aaccatgcat aaagcgggtg cattcgctgc cgcataccat tattcttgat ctgacggaag
    28201 tctttttgta acaattcaaa cttccttgat gtaaataaat caatacaaca aacggaattg
    28261 caaacttaca cacgcatcac tgcgtagatc aaaaaaacaa tcaccgcacg aggtttcatg
    28321 atggaaggtc aacagcacgg cgagcagcta aagcgcggcc ttaaaaaccg ccatattcag
    28381 cttatcgcgc tgggtggcgc gatagggaca gggctattcc taggtagcgc ctccgtaata
    28441 cagtccgcag ggccagggat tatcctgggt tacgccattg ctggttttat cgcctttctg
    28501 atcatgcgtc agctgggtga aatggtggtc gaagaacctg tcgcaggctc ctttagccac
    28561 tttgcttata aatactgggg cagttttgcc ggtttcgcct ctggctggaa ctactgggta
    28621 ctgtacgttt tagttgccat ggcagagctg actgccgtgg gtaaatacat tcagttctgg
    28681 tatccggaaa tcccaacctg ggtttctgcc gccgtattct ttgtggtgat taacgccatc
    28741 aacctgacca acgttaaagt gtttggcgag atggagttct ggtttgccat tatcaaagtt
    28801 attgcggtag tagcgatgat catcttcggc ggctggctgc tgttcagtgg caacggcggc
    28861 ccgcaggcga gcgttagcaa cctgtgggat caaggtggtt tcctgccgca cggcttcacc
    28921 ggactggtga tgatgatggc gattatcatg ttctcgttcg gtggtctgga actggtgggg
    28981 atcaccgcag cagaagctga taacccggag caaagtatcc cgaaagcaac taaccaggtt
    29041 atctaccgca tcctgatttt ctatattggt tcgttagccg ttctgctctc actgatgccg
    29101 tggacccgcg ttaccgccga taccagtccg tttgtgctga tcttccacga gttaggcgat
    29161 acctttgtgg cgaatgcgct gaacatcgtg gtactgactg cggcgctctc cgtgtacaac
    29221 agctgcgtat attgcaacag ccgtatgctg tttggtctgg cacaacaggg gaacgcgcca
    29281 aaagcgctgg cgtctgtcga taaacgcggc gtaccggtta acaccattct ggtgtctgcg
    29341 ctggttacag cattgtgcgt attgattaac tatcttgctc cggaatccgc atttggcctg
    29401 ttaatggcac tggtggtatc cgcactggtg atcaactgga cgatgatcag tctggcgcat
    29461 atgaagttcc gtcgcgccaa gcaggaacaa ggcgtggtaa ctcgcttccc tgctctgctt
    29521 tatccgctgg gtaactggat ctgcctgctg tttatggcgg cggtactggt gattatgctg
    29581 atgaccccag gaatggcgat ttcggtatac ctgatcccgg tatggctgat cgtgttaggt
    29641 atcggctatc tgtttaaaga gaaaaccgcc aaagccgtaa aagcgcatta atctctctac
    29701 gccctcaccc gtacagggtg agggcaataa tctctacacc aacgccccgt aaatcgtcag
    29761 tagcgcaatc accaccacga ccacgaacga ggttttcttc gccattgaaa ccgctgcctt
    29821 cggcgtctcc accttatcga catgcggttc acgcgccaga gagaactgcg ccagacgcgt
    29881 taatacctga tattgcgaag tatggaaatc acccagcgaa gcaaaccagg ccggtaacgc
    29941 tttctcacca tgaccgatca aggcatatac cacacccgca agacgaaccg gcacccaatc
    30001 cagtacatga agcactgcat caatgccgga ctgtaaacga tgatgcggcg tctggtatcg
    30061 cgccagccag tattgccatg cacgcaagaa cgcatacccc atcagcgtaa cgggtcccca
    30121 ggttcccccc acaatcagcc agaacagcgg tgcaagataa aaacgaaagt taatccacag
    30181 caatgcattt tgcagctcac gcaaatactc acgttcgtcg cagcctgccg ggacgccgtg
    30241 aatcatggtg agttcgccag ccatcgtggc acgggcatgg ctatcattac gtgaagcagc
    30301 tgtcagataa gcatgataat gaagacgaac tttacctgcg ccaatacaca gcaaaccaat
    30361 cagcagccac accagcagcg tgggaacgtt gaacaatact ccctgcaatg cgcgtaacag
    30421 taaaaaagtc acacccatcg caataatggt catgcctaac gtgcgcccga gagaaaaatg
    30481 tttcacccgc cgaaagaacg cttcaagacg atgatcaagc tgccagtgct cgcccaactt
    30541 aaacaggcgc tcgaaaatta acaccagtaa ggttgtaaat agcgtcatgt tgtctccttg
    30601 ctgaccagcg cacgaaaccg tgcccaatca aatgcaggac cgggatcggt tttccgatcc
    30661 ggcgcaatat cacaatggcc cgtcatgtta ttagcgatat cgggatagcg atctatcagt
    30721 gcccgcgtaa ccgccgcaag ctgttgatac tgcgcatcgg tatacgccag agtatcggtg
    30781 ccttcaagct caatcccaat agaaaaatca ttgcagcgtt cacgcccctg atactgagag
    30841 actcccgcat gccaggcacg tttatcgaaa ggaacatact ggactatttc accatcacgg
    30901 cgaatcaaac agtgagcgga gacgcgcaaa tgggcgatct cagcaaagaa aggatgtgcc
    30961 tgcggatcaa tagttccagt gaataatgcg tcgatccacg gaccgccaaa ctcgcctggc
    31021 ggcaggctaa tattgtgcac caccagcagg gtgggtgttt cgtcatccag gcggcaatcg
    31081 taatgtggtg agggaacgcg gcgcgcgcca accagccacc cctgttctaa caacatgcag
    31141 gatctcctta tatgtggtgc taatacccgg ttcagagtag catgtttcta ccttatgatt
    31201 cgttagctat ctggagtttt aacatgccgc ctcgccgcta caaccctgac acccgacgtg
    31261 acgagctgct ggaacgcatt aatctcgata tccccggcgc ggtggcccag gcgctgcggg
    31321 aagatttagg cggaacagtc gatgccaaca atgacattac ggcaaaactt ttaccggaaa
    31381 attctcgctc tcatgccacg gtgatcaccc gcgagaatgg cgtattttgt ggcaaacgct
    31441 gggttgaaga ggtgtttatt caactggcag gcgacgatgt caccataatc tggcatgtgg
    31501 atgacggcga tgtcatcaac gccaatcaac ccttgttcga acttgaaggc ccatcccgcg
    31561 tgctgttaac gggcgaacgc actgcgctta attttgtgca aaccctttca ggagttgcca
    31621 gtaaggtacg ccactatgtc gaattactgg aaggcaccaa cacgcagttg ttggatacgc
    31681 gcaaaacctt acctggcctg cgttcagctc tgaaatacgc ggtactttgc ggcggcggag
    31741 cgaatcaccg tctggggctt tctgatgcct tcctgatcaa agaaaaccat attattgcct
    31801 ccggctcagt gcgccaggcg gtcgaaaaag cgtcctggct gcatccggat gcgccagtag
    31861 aagtcgaagt agagaatctg gaagaacttg atgaggccct gaaagccgga gccgatatca
    31921 tcatgctgga taacttcgaa acagaacaga tgcgcgaagc cgtcaaacgc accaacggca
    31981 aggcgctact ggaagtgtct ggcaacgtca ctgacaaaac actgcgcgaa tttgccgaaa
    32041 cgggcgtgga ctttatctcc gtcggtgcgc taactaaaca cgtacaagca ctcgaccttt
    32101 caatgcgttt tcgctaattg ctgacagatt tatccgctcc atcgcgggcg gataatctcc
    32161 ccttccccaa ctttcttcgt aacgcctcgc aaatttcttg tctttcgctg caacgaaata
    32221 aaaaacctcg gaatctgctt tcccgtttcg ttttttgccc ctcgcctgtg ctgatggcgt
    32281 ttgccaaagt agcaccaacc aaatcaagga gcgaaacaga tggacaagca acgcggtttt
    32341 acacttatcg aactgatggt ggttattggc atcattgcca ttttaagctc cattggtatt
    32401 cccgcttatc aaaactacct gcgcaaagcc gcactcaccg acatgctaca aacctttgtg
    32461 ccttaccgta ccgccgtaga gttgtgcgcg ctggaacatg gtggattaga tacctgcgac
    32521 ggtggcagca atggcattcc ctcgcctacc accacccgct atgtttcagc catgagtgtg
    32581 gcaaagggcg tggtgtcgct gaccgggcaa gaaagtctca atgggctaag cgtcgtcatg
    32641 acgccaggtt gggataacgc aaacggcgtc accggctggg cgcgcaactg caatattcaa
    32701 agtgacagcg cattgcagca agcctgcgaa gatgtcttcc gctttgatga cgccaactaa
    32761 ggagcggcaa tgaatattcc acagctcacg gccctgtgcc tgcgttatca gggagtcttg
    32821 ctggatgcca gcgaagaagt ggttcatgtt gcggtggtcg atgccccctc acatgagttg
    32881 ctggacgcat tgcatttcgc taccaccaaa cgtattgaga tcacctgctg gacgcgccaa
    32941 caaatggaag gtcacgccag tcgcacacaa cagacattac ccgtagctgt tcaggagaag
    33001 catcagccca aagcagaatt gctgactcga acgttacaat ctgcgctgga acaacgcgcg
    33061 tctgatattc atatcgaacc agcggacaat gcctaccgca tccgcttgcg tatcgacggc
    33121 gtattgcatc ctttaccgga tgtttcaccg gatgccggag tcgcattaac agccagatta
    33181 aaagtgctgg gaaacctgga tattgcggaa catcgcctgc cgcaggacgg gcaattcact
    33241 gtcgaactgg caggaaacac cgtctcattt cgtattgcga ccttaccatg tcggggtggt
    33301 gaaaaggtgg tattaaggtt gttacagcag gtgagccagg cactggatgt taacacgctg
    33361 ggaatgcagc cgttacaact ggcggacttt gctcatgcct tgcaacaacc acagggactg
    33421 gtgctggtaa ctggccctac aggcagtggc aaaacggtca cctggtgttg tccacgctac
    33481 acaccaattc caccactgaa acgctggtac gtttacagca aatgggggtc gcccgctgga
    33541 tgctatcatc ggcgcttacg ctggtaatag cccagcgtct ggtacgtaaa ctttgcccac
    33601 attgtcgccg acagcaaggg gagcccatcc atattccaga cactgtatgg ccgtcgccgc
    33661 tgccccactg gcaggcaccc ggttgtgtac attgctacca cggtttttat ggtcgtaccg
    33721 ccttgtttga agtcctgccc attacaccag ccatacgcca gcttatttcc gctaatactg
    33781 aagttgaatc gctggaaacg cacgcccgac aggcgggtat gcgtacgctt tttgaaaacg
    33841 gctgcctggc cgtggagcaa ggcttaacca cctttgaaga gttaatccgc gtactgggga
    33901 tgccgcatgg caagtaagca actctggcgc tggcatggca taaccggcga cggcaatgcg
    33961 caagatggga tgctatgggc agagagccgt actttactgc ttatggcact acagcaacag
    34021 atggttaccc cactaagcct gaagcgaatc gccatcaatt ctgcgcagtg gcgaggagat
    34081 aaaagcgcgg aagtcattca tcaactggcg acgctactta aagccgggtt aacgctttct
    34141 gaagggctgg ctctgctggc ggaacagcat cccagtaagc aatggcaagc gttgctgcaa
    34201 tcgctggcgc acgatctcga acagggcatt gctttttcca atgccttatt accctggtca
    34261 gaggcatttc cgccgctcta tcaggcgatg atccgcacgg gtgaactgac cggtaagctg
    34321 gatgaatgct gctttgaact ggcgcgtcag caaaaatccc agcgtcagtt gaccgacaaa
    34381 gtgaaatcag cgttgcgtta tcccatcatc attttagcga tggcaatcat ggtggttgtg
    34441 gcaatgctgc attttgttct gccggagttt gccgctatct ataagacctt caacacccca
    34501 ctaccggcac taacgcaggg gatcatgacg ctggcagact ttagtggcga atggggctgg
    34561 ctgctggtgt tgttcggctt tctgctggcg atagccaata agttgctgat gcgccgcccg
    34621 acctggctta tcgcgcggca gaaattgctg ttacgcatcc cgattatggg ttcactgatg
    34681 aggggacaaa aactcacgca gatctttacg attctggcgc tgacacaaag tgcaggcatt
    34741 acttttttac agggcgtaga gagcgtcaga gaaacaatgc gctgcccgta ctgggtgcaa
    34801 cttctgacac aaatccagca cgatatcagt aacggtcatc ccatctggct ggcgctaaaa
    34861 aatgccggtg agtttagccc gctctgtttg caattagtga gaacaggaga ggcatccggc
    34921 tcgctggacc tcatgttaga caacctcgcc catcatcatc gggataacac aatggcgctg
    34981 gcggataacc tcgcagcctt actggaaccg gcgttgctga tcataacggg aggaattatc
    35041 ggtacgctgg tggtggcaat gtatctgcca attttccatt taggcgatgc gatgagtggg
    35101 atgggataac gctggcgtgt tgctccacgc cagcgttggg agattacagg ttgttgaaga
    35161 tgcggttttc ttgttccagc acacgaataa acgtggtgcg cttggtcagc tctttcaggc
    35221 gtgaagcccc aacgtatgta caagctgaac gcaggccgcc caaaatatca cgcgcggtat
    35281 tttcaacctg gcctcgcagc ggcagcttaa cggttttacc ttctgctgcg cggtattccg
    35341 caacgccgcc aacgtgacgt ttcatcgcag actcggagct catgccgtag aacagcataa
    35401 atttctcgcc gttctcctca acgatgcgac cgccgctctc ttcgtggccc gccagcatgc
    35461 cgccaagcat gacgaaatcg gcaccgccgc caaaggcttt cgccacatcg cccggcgtgg
    35521 tacagccacc gtcgctgacg atcattccgc ccagaccgtg cgccgcatcg gcacattcga
    35581 ttaccgcaga aagctgcgga taaccgacgc ctgttttgac gcgagttgta caaacagaac
    35641 ctgggccaat gccaacttta acgatatcag caccggagag gataagctcc tcacacattt
    35701 caccagtcac tacgttgcca gcacaaatgg ttttggtcgg ccacgcttca cgcgctttcg
    35761 caacgaactg cacgaagtgt tcggaataac cattcgccac atcaatacaa acgaagttta
    35821 atgccgggtt caggtcgaga atctgtttag ttttttcgaa atccgcatca gacgtgccgg
    35881 tagaaaccat cacatgtttc agcacatcag cggaagaatt gttgataaac gcttgccact
    35941 cttcgacaga aaagtgttta tgcacagcag tcagaatatc aaaagaagcc agcgcagagg
    36001 ccatagaaaa tgtgcctacg gtgtccatat ttgcggcgat aatcggcacg ccggaccagc
    36061 tctgacctga atgtttgaag gtgaattgac gttccagttc aacatcggaa cggcttttaa
    36121 gagtggagcg tttagggcgg atgagaacgt ctttaaaacc taacttcaga tcttcttcaa
    36181 tacgcatgtg cggattcctg gggttaatgg ctataagggt aagagcgcaa ctccagtgac
    36241 gctatcatac gcactaatca atgcggcgca agactgcgaa attgtctttt tttacgctac
    36301 aatcccataa atttacctgg taaatagtga gcgaacgttt catcaatttc actttcgcgc
    36361 gctcattttt aacgttttga taatcatgat gaattcccgg gaatataaat tatggggtat
    36421 atagttgcct taacgggagg cattggcagt ggtaagagta ccgttgccaa tgcgtttgct
    36481 gatctcggaa ttaacgtcat tgatgccgat attattgcgc gtcaggtggt tgaaccaggt
    36541 gcacctgcgc tacatgccat tgctgatcac tttggcgaaa acatgattgc tgctgatgga
    36601 acattgcagc gccgggcctt gcgcgagcgg atcttcgcca acccggaaga gaaaaactgg
    36661 cttaacgacc tgctgcatcc gctgattcag caagagacgc aacaccagat ccagcaagca
    36721 acttccccct atgtactgtg ggttgtgcca ttgctggtag aaaactcact gtataaaaaa
    36781 gcgaatcgag tgctggtggt ggatgtcagc ccagaaacgc aacttaagcg caccatgcag
    36841 cgcgatgatg taactcgcga acatgtcgaa caaatccttg ctgctcaggc aacgcgcgaa
    36901 gcccgccttg ccgtggcaga tgacgtcatt gataataacg gcgcaccgga tgctatcgca
    36961 tcggatgttg cccgcctgca cgcacactat ttgcagcttg cgtcgcagtt tgtctcacag
    37021 gaaaaaccgt aatgcagacc caggtccttt ttgaacatcc actaaatgaa aaaatgcgta
    37081 catggctgcg cattgagttt ttgattcagc aactcaccgt taatttaccc atcgttgacc
    37141 acgctggcgc gctgcatttc ttccgtaatg tcagtgaatt actggatgtt ttcgagcgcg
    37201 gcgaagtccg cactgagctg ttgaaagaac ttaaccggca gcaacgtaaa ctccagacct
    37261 ggattggcgt gcctggcgtg gaccagagcc gtattgaagc attaattcag cagttaaaag
    37321 cggcggtgag cgtattaatt tccgcgccgc gtatcgggca atttctgcgt gaagatcgtt
    37381 tgattgctct ggtgcgtcag cgactgagca tcccaggcgg ctgttgcagc tttgatttac
    37441 ctacattgca catttggctg catctacccc aggcgcagcg cgacagccag gtagaaacct
    37501 ggattgccag cctgaacccg ctcacccagg cacttaccat ggtgctggat ttaattcgcc
    37561 agtcggcccc cttccgtaaa caaaccagcc tgaatggttt ttatcaggat aacggtggcg
    37621 atgccgactt gctgcgcctg aatctgtcgc tcgattcaca gctttatccg caaatttccg
    37681 gacataagag ccgttttgcc attcgtttta tgccgctgga cactgaaaac ggacaggtac
    37741 cggaacgtct ggatttcgaa ctggcctgtt gctaaggagt aaaaatgtca gaaactatta
    37801 cggtgaattg cccaacctgc gggaaaacgg tggtgtgggg tgaaatcagc ccgtttcggc
    37861 cattttgctc caaacgttgt cagctgatcg acctcggaga atgggctgct gaagaaaaac
    37921 gaatccccag cagtggcgat ctttccgaaa gcgatgactg gagcgaagag ccaaagcagt
    37981 gacatttgtc tgatgcctca cgtaggcctg ataagacgcg gacagcgtcg catcaggcat
    38041 cttgtgcaaa tgtcggatgc ggcgtgaacg ccttatccga cctacagacg cttaagcttc
    38101 gcaattaccg gttcattggc tggcggaaaa tcatcggcat taagaccgac cagcgacatc
    38161 cactcaccgg gttgcccttc tttaccccac ggcacccctt cccaactttc gaccagccag
    38221 aaccacagag ttatatgcct gtccgggaat tcatattcca gtttttcaaa tagcgaaaaa
    38281 tgttgtgggg taatcccgac ctcttcctga agttcacgca ccaccgcctg ttccggcgtt
    38341 tcgcccattt caattttacc gccgggaaac tccagtttat tcgccatgtg cgcatctgct
    38401 gcgcgacgtg ttataaagat ttcattgttc tcgttgcgaa taatacctac cgcaatttgc
    38461 agctttttca ttgtcttaaa cctataaaaa aggcgcagaa tcctgcgcct tttacttcaa
    38521 ctgttagctt ttattgcagg cgaccatggc actgcttgta ttttttacca gaaccgcacg
    38581 ggcaaggatc gttacgtcct actttgcgtt caccggtttg cgccgccagt gcagctgcgg
    38641 ctgcagagtc gtcatcctga tggctaagct gctgcatttg tgctaaacgc tcggcttcca
    38701 tacgacgctg ttgttccagc tcctcaacct cttcaggcat acgtacctga actttgctca
    38761 gcgtactgat aacttcatat ttcaacgact ccagcatcgc tgcaaacatg gagaacgatt
    38821 cacgtttgta ttcctgcttc ggatctttct gtgcatagcc acgcaggtgg ataccctgac
    38881 gcagatagtc catcgctgcc aggtgctctt tccacaggga gtcaagcgtt tgcagcatga
    38941 cgcctttctc gaagtgacgc atcatctcag caccaaccac ttcttcttta cgctgataca
    39001 cttcgatgga ctgcgccaga atgcgctcac gcagcgtctc ttcatgcagt tctggttctt
    39061 tatccagcca ctcggcaatt ggtaaatcga ggtcgaaatc gttcttcaga cgttcctgca
    39121 gccccggaat atcccacatt tcttccagcg actgtggcgg aatgtaggca tcaatggtgg
    39181 ctttgaacac atcttcacga atgctgttga tggtttcgct cacatcgctg acatccagca
    39241 gttcgttacg ctgggagtaa atggcgcgac gctgatcgtt agccacgtca tcatattcca
    39301 gcagttgctt acgaatgtcg aagttacgac tttcaacttt acgctgggcg ttggcaatcg
    39361 ctttagtcac ccacgggtgt tcaatggctt cgcctggctt catacccagt ttacgcatca
    39421 tgccggatac tcggtcggaa gcaaaaatac gcatcagcgc atcttccatc gacaggtaga
    39481 aacgggaaga accagcatcc ccctgacgac cagaacgacc gcgcaactgg ttatcgatac
    39541 gacgggattc gtgacgctcg gtaccgatga tatgcaggcc acctgcttcc agtaccgcat
    39601 cgtgacgtac ctgccagtcg gctttaattt tttcaatttg ctctgcggtc ggattttcca
    39661 gcgcggcaac ttctgcctgc cagctacctc cgagcacaat atctgtacca cgacccgcca
    39721 tgttggtcgc gatagtcacc gcagccggat aacctgcctg agcaacaatc gccgcttcgt
    39781 tggcgtggaa tttggcgttc aggacattgt gcttaatacc ggctttggtc agttcgtttg
    39841 acaccagctc cgatttttcg atggagatag tacccaccag caccggctgg cctttcgcag
    39901 tacgttcttt gatatcttca atgatcgcct gaattttttc cgcttcagtc atgtagacca
    39961 ggtccggcag atctttacga atcattggac ggttggtcgg aacaacgacg gtatccagtt
    40021 tgtagatgga gctaaattcg aaagcttcgg tatcagcagt accggtcatc cccgccagtt
    40081 tttcatacag acggaagtag ttctggaagg tgatcgaagc cagcgtttgg ttttcgttct
    40141 ggatctgcac accttctttc gcttccacag cctggtgcag accatcggac cagcgtcggc
    40201 cctgcatggt acgaccggtg tgttcgtcaa cgatgataac ttcaccatct ttaacgatgt
    40261 agtcgacgtc acgggtaaac agcgcatgag cgcgcagcgc cgccgttacg tggtgcatca
    40321 gcatgatgtt ggccggagag tacagagact ccccttcatc catgatgccc tctttcacca
    40381 gcagttcttc aatcagcacc agaccacgtt cggtcaggtt cacctggcga gatttttcgt
    40441 ccaccgagaa gtggccttcg ccctggaagg tttcggagtc ttctttttcc tgacggatca
    40501 ggtgcggaat aattttattc acgcgtttat acatttccga gctgtcttct gccgggccgg
    40561 aaatgatcag cggtgtacgc gcttcatcga tcaggatgga gtccacttcg tccaccagcg
    40621 catagtgcag tttacgctgt acacgttctt cagggctgaa cgccatgttg tcgcgcaggt
    40681 agtcaaagcc gtattcgttg ttcgtaccgt aagtgatgtc agctgcgtaa gcttcgcgct
    40741 ttgccggtgc tggcatgccc ggcaggttga taccaacagt caggccaagg aattcaaaca
    40801 gcggacggtt gttttcggcg tcacgttgcg ccaggtagtc gttgacggta actacgtgca
    40861 cgcctttacc ggttagtgcg ttcaggtaag caggcagcgt tgcggtcagg gttttacctt
    40921 caccggtacg catttcggcg atgcagcgtt cgttaagaac cataccgccg agtaactgaa
    40981 cgtcgaagtg acgcatacca aagacgcgct tacttgcctc acgcaccacg gcgaaagctt
    41041 ccgggatcag attttccagc acttcgcctt tttccagacg cgcacgaaac tctgcggttt
    41101 tccctttcag ttcttcatcg gagagttttt ccatctccgg ttccatggca ttgatgatgt
    41161 tgaccacttt gcgcatccgg cgcagggtgc gatcgttacg actaccgaaa actttagtta
    41221 acaatttgat tagcataata aaatctcaaa cgccccgcgt tacggagtta ataaaatgaa
    41281 gtaaaggttt attgttgtta ggtgaggcgt tgagggccag cacggatgcc ttgcgcctgg
    41341 cttatccaga cgggcgtgct gaattttgct tgtggggtaa aatgcgcata atcaatgcga
    41401 taaccctttt cagacggcgt gccttcctgg gtcagcagcg cgctgagcgt atccagtaat
    41461 gcaagatgtt gcgcctgaag aggcaaagat tcttcagcaa cgggcagtgt ttgcggtgcc
    41521 attgcgaaag aaagatgacg gattaccgta cgaatagcat gttgatgcca gtaatcaacg
    41581 gaatagttcg aattcgggcg gcgcgtgttc gcttccagca aggccaattg accaaagtta
    41641 actttggctg aaggctcgtg gttgcgggtt gtcgcttttg cgggcgcgtt tggttcggcg
    41701 gcgttgctga gcgcaggcaa acctaaactc gccgcgacca tccctaataa gagatgcggc
    41761 cagaagtagc gtttaccaaa ctgtcgccag cgcgtcagta ttccactcac gttattgcca
    41821 tcccgatcga attcaaacgt atttagcgca caaatcttat cattaaagcc tgaagtccac
    41881 agcacgaatg aagataagtt gtgcgtaaaa gtgcttaaga aaacagcgtt cgcaccgttt
    41941 tttatagctc ttataaaatt caaaaaaaaa cgactggttc acctggccgg agagagtgcc
    42001 agatttgcca gtcgaatttt atacgacagt ataaatgtcg ttatgccagt acagctgaag
    42061 gcgctttgaa ggccaacggc agttctgcgt cgtcctggaa ggtcacatat tcccaggctt
    42121 cctgtttcgc caggacagcc tgcagcagtt tgttattcag tgcatgaccg gatttataag
    42181 cggtaaatgc accaataata ttgtgaccac acatgaacaa gtcaccgatc gcatcgagca
    42241 ttttgtgacg cacaaattcg tcttcaaaac gcaggccgtc ttcgttcagt acgcgataat
    42301 cgtcaacaac gatggcacaa tcgaagctgc cgcccaggca caaaccacgg gactgcagat
    42361 attcgatatc acgcatgaaa ccgaacgtac gcgcacggct gatctggcgc ataaacgcat
    42421 cagcggagaa gttcatcgca tagcgctggt tgctggaatc aatagccgga tggttaaaat
    42481 cgatggtgaa atccagcgaa aaaccattgt acggcttaaa ttcagcccac ttatcgccat
    42541 cttcgacacg aacagtctct ttgatgcgaa caaatttttt ggcgcagttc aactcgtcga
    42601 taccggcgtc aagcagcagg tatacaaacg gagcggcgct gccgtccatg atcgggattt
    42661 ccggcgcgtt aacttcgata acaatgttat cgatgcccag gcccgcgaga gcagcattga
    42721 ggtgctctac ggttgaaatc cgtacatcat gctcgttgac cagacacgta cagagcatgg
    42781 tatcacgcac agatttggca tcggccggga aatctaccgg tggattcaag tcggtgcgac
    42841 gatagatgac cccggtgttg gccggcgcag ggcgtaacgt cagggtgact ttcttgccgg
    42901 tatgtaaacc gacacccgtc gcctgaacga tacgtttaag tgtcctttgt ttgatcatcg
    42961 tattatctcg ccaaattacc tatccaaccg aagtgtacta tacattcggc gggccagttt
    43021 agcacaaaga gcctcgaaac ccaaattcca gtcaattctt aatcagcttg cttacgtagg
    43081 aatgctggga tatccagata atccggctct ttcgcagttt gcggcgcatt gtcattcact
    43141 actttagcaa ccggcttctg ctcctgggtc agcggagcca tcccatgctg ctggtagcga
    43201 tccatcactg gctgctgaac ctgcttattg gtcaccagag tgatttcagg acgtttgtcc
    43261 atgccgatac ctgtcgcaac aacggttacg cgcagctcgt cattcatatc cgggtcaaga
    43321 gaagtaccga taaccacagt cgcgttgtcg gaagcaaatg cacggatggt gttacctacc
    43381 gtttcgaact catccagacg caggtcgaag cccgccgtga tgttaaccag cacgccgcgc
    43441 gcgccagaca ggtcgatatc ttccagcagc ggagaagaga tagccatttc agcagcttct
    43501 tccgcacggt cttcaccgct cgccacgcca gaacccatca ttgcgtagcc catttcagac
    43561 attacggtgc gtacgtctgc aaagtccacg ttcatcaaac ccggacgagt aatcagttcg
    43621 gcgatacctt gcacagcgcc tttcagtaca tcgttcgctg cgccaaacgc atccagcagg
    43681 gagataccgc ggcccagaac tttcagcagt ttgtcgttcg ggatagtgat cagagagtcc
    43741 acatgcttgg acagttcagt gatcccctgc tcggcgaatg ccatacgctt cttgccttca
    43801 aagttgaaag gcttagtgac gacagcaacg gtcaggatac ccaaatcttt tgccacttca
    43861 gcgacgactg gtgctgcacc tgtaccggta ccaccaccca tacccgcagc aataaagacc
    43921 atgtctgcac cttccagcgc cgcacgcaat gcatcgcgat cctcatcagc cgcattgcgg
    43981 ccaacttctg gattagcgcc agcgcccagt cctttggtga taccgctacc gatttgaatc
    44041 gtctgtccaa ccgctgtttt acgcagcgct tgtgcatcgg tatttaccgc gaagaattca
    44101 acaccttcaa tacgctcgcg caccatgtgt tcaacagcat taccgccgcc gccgccgacg
    44161 ccgatgactt taatcaccgc gtcattggta agttccattg gttcaaacat agtttctctc
    44221 cgatttgtgc ctgtcgcctg aggccgtaat catcgtcggc ctcataaaaa ttaaaactct
    44281 tttcgcagcc aactattgag tcgcttgatc cacgagccaa ctgatgctgt aacacgtttt
    44341 tctacttcag cttcaccgtt aagatgtgac tctttcccat agtgaagcaa tcccaccgcc
    44401 gtcgaataat acggctcctg agcataatcc gttaaaccgg taatgttcag cggcgcgccg
    44461 atacgcactt gcgtatgaaa cacgcgctga gcacaggctg caagaccttc gatctgcgct
    44521 gcgccaccgg ttaatacaat gcctgccgcc aggtgatgtt taaccccttg ttggcgaagc
    44581 ttttcctgca actgcaatat ctcttcgttg accaggttga gcaactcggt atagcgcggc
    44641 tcgatcacct ctgccagggt ctgacgttgc agactccgcg gcggacgacc gcctacgctc
    44701 ggcacttcca cgctctcatc ttttccaacg atggaaccca gcgcacaacc gtggcgaact
    44761 ttaatcgctt cggcgtcgct tggcggcgtg ccaaaggcgt aagcgatatc actggtcacg
    44821 acattgccag cataaggaat tacctttgtg tggcgcaatg ccccaccggt ataaacggcg
    44881 atatccattg taccaccacc gatatcgacg acgcagacac ccagttcacg ttcatcttcc
    44941 gtcaataccg aataacttga tgccagtccg gcaaatatca gttggtcaac tttcagccca
    45001 caacgttcaa ccgctttgac gatgtttttc gccatatcgt tgtgacatgt gatcaggtgc
    45061 acttttgcct gcatccgcac acccgaaagt cctaccggat tcttgatccc ttcctgatag
    45121 tcaatcgcat actcttgcgg gatcacatgc agcacacgat gctcatcgcg cacacgcacc
    45181 gatttcgcgg tatggacgac gttttccaca tcttcttgcg tcacttcttc ttcagaaata
    45241 ggcaccatac caatttcatt ctggcagctg atgtgcttac cagaaagcgc cagatatacc
    45301 gaagagatct gacaatctgc catcaattct gcctggtcaa tggcgcgttg tacgcacttg
    45361 accacggatt cgaggtcgtt caccccgcct ttatccatac cacgtgacgg gcagctgccc
    45421 acgccaatga tattgaccat accgtcgggc agaacttccc ctactaaagc ggcaaccttc
    45481 gcggtaccaa tttccagtcc tactaccagt tttctgtccg tcgccttgat cattgttgtt
    45541 ctgcctgtgc ctgattttgt tgctgagtag attcctctgg cggcaagggc gcccagccta
    45601 ctgccgctcc agagtcataa cgcaaatcaa cgtagctaat ccgtttgcca tcggtttgcg
    45661 cctgctgctg taaaaccgga taaagttcta caaagcgaac caaacgtttc atcgtatcgc
    45721 cccggccaag attgagctta atatcgttat tcagcgtcaa ctgccaggaa cgccgcgcgg
    45781 tcatcgccgc ttccttcaga gtaaatctgt cctttgccag catctgcccc atttcgcgat
    45841 agccctgcaa cacttcatta gcgctgcctt ccgggccata cagcattgga agcacctgct
    45901 tgctggtgcg atctggcggc acgctgaagg tgtttccttc cgcgtctacc atgtgttgat
    45961 cattccaccg cgcaatcggc acatattcaa ccagatgaat cttcaattca tcaggccact
    46021 gctttctgac gctcacctgc ttaatccacg gcaggcgttg ttctatttgc gtctggatga
    46081 tgttgacatc ctgggtcata aaggtacccg gctcacccaa tgccaggatc gactgccgga
    46141 tatcgtcatt acgcgtgtaa tggcgttcac cggtcaacac cagctttgag agcggcaggc
    46201 gttgcgcatc ttccatccag ctcaacacga cccagccgct caccaacact gtcgttaaaa
    46261 cggtcagcag gaaaaggatc cccgccagac gcgttccatt attgcggcga gaagaaacct
    46321 cttcttcgct gtttcgcgtg ttcagagcag cctgcgacat attagtccgc cagttccaga
    46381 attcgtacta ccaactgcga gaagctcata cctgcctgac gtgccgccat cggcaccagg
    46441 ctgtggctgg tcatacccgg tgaggtattg gcttccagca gataaaactg tccatcactg
    46501 tccagcataa cgtcaatacg tccccatcct ttgcaaccta acgtcgtcca tgctttcagc
    46561 actaatgcct gcaaattggc ctcttgtgac gcttccagac ctgcggggca gaaatactgt
    46621 gtctcatcag agagatactt cgcctcataa tcatagaagg ttccggacgg ttgaatacgt
    46681 attgacggta aaatttcttc accgagtatc gcaaccgtga actccggccc acttagccat
    46741 ttttcaatca atacttcttc atcgtgctga aatgccaatc ttaatgcatc ttgtagagca
    46801 ttttctgcta ctacttttga cattcccaca ctggaacctt cgcggctcgg cttaacgata
    46861 accggcaaac ccagagcaga aatttctgct aactgcttat cgctcaggcc tttttcaaac
    46921 tctgcgcggg ttaacgctac ccacggcgcg accggtaaac cggcaccttg ccacagaagt
    46981 ttgctgcgta gtttatctat tgaaagcgca gatgccatca ctccgctgcc ggtataaggc
    47041 aagcccatca gctcgagcat gccctgcagc gtaccatctt caccgccgcg accgtgtagc
    47101 gcgataaaca ctttctgaaa acccatcgac ttcagttgcg tcacgtcgac ttctttcggg
    47161 tcgacaggat acgcatcaat accgccttca cgcagcccgg ctaacaccgc tgcgccagaa
    47221 ttcagagaaa cttcccgctc agcggaagtc ccgcccaaca ggaccgcgat tttatcagtc
    47281 atgttgttct tcctccggag tttgcggctt cagtttgatt tcagctaaag aacgggcaat
    47341 ttttccgata ttaccagccc cctgaacgag aatcaggtcg ttaccggtta ataccggtgc
    47401 cagcatctcg gctacctgcg cagggtccgg caccagaatg ggatcaattt tcccacgtcc
    47461 acgaattgtg cgacacagcg aacggctgtc cgctcccgga attggcgctt cgccagccgg
    47521 atacacttcc agcatcaaca gggtatcaac ctgcgtcagc acattggcga aatcatcata
    47581 caggtcgcgc gtacgggtaa aacggtgcgg ctgaaacagc attaccaggt ttttatccgg
    47641 ccagcctgcg cgcgccgctt taatggtggc gtcaacttcc gtcgggtggt ggccgtagtc
    47701 atcgaccagc attgccgtac cgcttttacc attcactggc tccagcggga attcaccgag
    47761 gaaatcaaaa cggcgaccag tcccctggaa gctctccagc gcacgcaaaa tagcctcgtc
    47821 gtcaattccc tcttccgtag caaccgcaac cgcagctgcg gcgttcagcg cgttatgacg
    47881 gcctggcgca ttcagggtga cgcgcatcgg ctctttgtcc tgacgcagca gcgtaaagtg
    47941 cccctgcggg ccaatctgct gataatcttc tacacgcacg tcggcatctt cgctgaagcc
    48001 gtaagttgtg gtctgacgtc ccacacgcgg taacaattcg cggatcaccg gatcatcaac
    48061 acacatcacc gcacgaccgt aaaaaggcag gttgtgcaga aaattaataa aggtctgttt
    48121 taaattctca aagtcgccct ggtaggtatc catgtggtcg gcttcgatat tggtgacaat
    48181 cgccaccatc ggttgcagat gcaggaacga tgcatcactc tcatctgctt cggcaatcag
    48241 gtaccgacca tgccccaaac gcgcatgaac ccccgccgct tttaccagcc cgccgttaac
    48301 gaaggttggg tcgagccccg cttctgcgta gatgctggaa accatcgcgg tagttgtcgt
    48361 tttgccgtgc gttcctgcaa tggcgatgcc atgacgaaaa cgcattaact cagccagcat
    48421 ttcggcacga cggatcaccg gaatacgcgc ttcatgagct gcgacaattt ccgggttgtc
    48481 ggcagaaatg gcgctggaaa caacgaccac gctggcatca cgtacgtttt ccgggcgatg
    48541 gttgaaataa atcgtcgcac ccagattcag taactgctgc gtgaccggat ttggcgctaa
    48601 atcggaacca ctgatctgat aaccttcatt ggccagaact tcggcaatac cgcccatacc
    48661 ggcaccacca atgccgacaa aatgtatgtg ccgaacgcga cgcatttcgg gcacgatgga
    48721 acgcagtttt gccaattgtt gtgtattcat tctttacgcc attaacttct taaattcata
    48781 cgatgcaaaa ggcatcgcta caattacgcc cgggcagccc ggctcacttc atttgccact
    48841 cgctcggtgg catccggaat ggatgcagcg cgggcgcgtt ctgccatggt taataaggtt
    48901 tctcgcgacc acccggccag ggtgttggcg acagcatcca cgctaagctg tggctgctca
    48961 ataattttgg ctgcgcccgc tttttccagc ggtagcgcat tccagtattg ctggcggtct
    49021 ttatgttgaa acggcacaaa caacgccggt agtcctgccg cggcgatttc actcacagtt
    49081 aaagcaccgg agcggcaaac gacgacatcc gcccacgcat acgccgccgc catatcatca
    49141 ataaattcag tcactttatg ctgcggttgc cccgcttcgg catacgcctg ttcaacggat
    49201 tgttgcgaac ctttgccgct ctgatgccag atagtgactg aatcacccag tttcgcagca
    49261 acctgcggca ttgtctggtt aagaatgcgt gcgccctggg aaccacccac taccaacaca
    49321 cgaaccggac cttcacgtcc agccaaacgt tgctgcggca acggcagcgc cagcacatcg
    49381 gtacgcaccg ggttacccac cacttccgca ttagggaaag cgcctggaaa cgcctgcatc
    49441 actttggtag caatcttcgc cagccattta ttggttaagc ccgcaatacc gttttgttca
    49501 tgaagtacaa ccggaatgcc taacgaccac gcggccagac cacctggacc tgatacatag
    49561 ccgcccatac cgagtaccac gtcaggtttg tacgctttca taatcgcccg cgcctgacgc
    49621 caggcgttga agatacgcag cggcgcagct atcagtgctt ttataccttt tccacgcaaa
    49681 ccagagatac gaatgaaatc aatttcgatg ccatgttttg gcactaagtc cgcttccata
    49741 cggtcggcag tccccagcca gcgaacttgc caaccctgag ccattagata gtgcgcaacc
    49801 gccagtcccg ggaatacatg tccaccggtt ccgcctgcca tcaccattaa tcgctttcct
    49861 tgaccactca tcgtgaacct cgtacaaacg cctgcgcttt ctccagacgc gtttcataat
    49921 caatacgcaa cagcatcatg atggctgtcg acataatcag taagctcgaa ccaccgtaac
    49981 tgattagcgg caatgtcaga cctttggtcg gtaacatccc cgccgccgcg cctacgttaa
    50041 ccagcgcctg gaagctaaac cagatgccaa tagaacaggc gagaaaaccg gaaaaacggt
    50101 ggtcaatttc taatgctttg cggccaatcg acatcgcacg aaaagcgacg aagaatacca
    50161 ttaaaagtgc tagtaccaca ccgacataac ctaattcttc gccgataatg gcgaaaataa
    50221 agtcagtgtg cgcttccggc agatactcca gtttttgtac cgaattacct aaaccttgcc
    50281 cccaaagttc gccgcgacca aacgccatca gcgattgcgt taactgatag ccgctgccaa
    50341 agggatcttc ccacgggttc cagaatgcgg taacacggcg gatacggtac ggttcggcga
    50401 ggatcagcaa cacaaccgct gaaatgccca taccgataat ggcaatgaac tgccacaatt
    50461 tcgctcccgc caggaacaac atcgccagcg tagtcacaaa caacaccacc accgtaccaa
    50521 ggtctggctg tgccagcagt aacactgcca acaccagaat cacgcccatc ggtttcagga
    50581 agccgcgcag gttattacgt acttcgtcgc ctttacgcac cagatagttg gcgatatagc
    50641 aaaacagcga cagttttgtc agctccgcag gctggatacg cagcaaaccg agatcgatcc
    50701 aacgcgatgc ccctttaaca gcgctaccca ctaccagcac gatcatcagc aggatgatag
    50761 atccgagcag catcgtggca ctgtagcgtt gccagaactc catcggcaga cgcagcgtaa
    50821 tgatcgccag aataaacgcc aaaatcagat agacaccatc acgcttcgcg aagaagaacg
    50881 gatcgttggt taagcgttgc cctatgggca ttgacgccga ggtcaccatg ataaagccaa
    50941 tcgccgcgag gccgaaggtc agccacagta aggtgcgatc gtacatgatc aggctgtcgg
    51001 tatctttttc ccgcgagccc atcacccagc cctttagcgc cgtagagatc cagaccagga
    51061 tactgaatcc tggcaggcgc ggcattttca ggcgagggag agataaacgc atcaacctaa
    51121 ctccttcgcc agacgggcaa actcattgcc tcgttgttca aagttcttga actgatcaag
    51181 gctggcacag gccggggaga gcagaaccat atcgcccggc tgaacacgcg gagccagcaa
    51241 gcgcatagcc tgttccatag tttcggtttg ttctgccact tccgggcgta gcgccgccag
    51301 ctgcgcgccg tcacgaccga aacaatacag acgtacgtta tcgccattca ggtaacgcac
    51361 cagtgggcta aagtccgccg atttaccatc gccgcccagc aacaaatgca gcgtgccgtc
    51421 tacgtgcagg ccattcagcg ccgcttccgt actgccgacg ttggtcgctt ttgaatcgtt
    51481 aatccagcgc acaccgttat gctccagcac cacttcaaag cgatgcggca gaccggtgaa
    51541 ggtggttaac gctttcaggc tgctggcgcg cggtaaccct gcggcatctg ccagcgccag
    51601 cgccaccagc gcattggtgt agttatgctg cccggaaagt ttcatctctt tcacattcag
    51661 cactttctcg cctttaaccc gcagccaggt ttcgccctgc tgatgattca ggtgatagtc
    51721 acccatgttg acgccaaagc tgacgcagcg ttcatccgca ccgcgaatcg gcattgttaa
    51781 ggcatcatca gcattaacca cgcaaacttt cgcgttttcg taaatgcgca gttttgctgc
    51841 acgatactgt tgtaaaccaa acgggtagcg atccatatga tcttcagtca cgttcagaat
    51901 ggtcgctgct accgcctgta agctggaggt ggtttccagc tggaagctcg acagttccag
    51961 cacgtacagt tcacactcat catccagtag catcaacgca ggcaggccaa tattgccacc
    52021 cacaccaacg ttaacccccg ccgctttcgc catttcaccc actagcgtgg tgaccgtgct
    52081 tttgccgtta gaaccggtaa tcgccacaat cggtgcttgt gcttcgcgac aaaacagctc
    52141 gatatcgcca acgatttcga ttccggcatc agcggcagcg cttaaggatg gatgcgccag
    52201 tgcaataccg ggactggcga caatcagatc tgccgccatc agccattcat cattcagact
    52261 gcccgtgtgg cgttctacgg cttcgggtaa tttatccagg ccaggcggtg tcatacgcgt
    52321 atccataacg cgcggcgtca caccgcgagc gaggaaaaag tccacgcagg aaagcccggt
    52381 gaggcccagg ccgataatga caacattttt accctggtaa tcagccatga ttaacgtacc
    52441 ttcagcgttg ccagaccaat caggaccagc atcagcgaaa taatccagaa acgcacaatg
    52501 acgcgcggtt ccggccagcc tttcagttca tagtggtgat gaatcggtgc catgcggaaa
    52561 atacgttgtc cgcgcagttt aaaggagccg acctgcagga tgacagaaag cgtttctacc
    52621 acgaacacgc cccccataat caccagcagg aattcctgac gtagcagtac ggcgataatg
    52681 cctaacgcac cacctaacgc cagcgaaccg acatcgccca taaagacctg cgccggatag
    52741 gtgttaaacc acaggaagcc cagtcctgcc ccgactatcg cggtacagac aataaccagt
    52801 tccccggcgt gtcgcagata cggtatgtgt aaatagctgg caaagttcat gttgccggtc
    52861 gcccacgcca ccagcgcaaa accaccggcg acaaatacgg tcggcataat tgccagaccg
    52921 tcaagaccat cggtcaggtt taccgcgttg ccagtaccca caatgacgaa gtaagccagc
    52981 agaatgtaga acagccccag ttgcggcatc acatctttaa agaatgggac caccagctgc
    53041 gttgcgggcg tgtctttgcc ggcaaggtac aaagcgaaag caacacccag cgcaatgacc
    53101 gacatccaga aatatttcca acgagcgatc aaccctttgg tgtctttacg caccactttg
    53161 cgataatcat caacaaagcc aataacaccg taacctacca gcaccaccaa cacgcaccag
    53221 acgtacggat tggacgggta agcccacagc agcacggaga tcacaatcgc tgtcaggatc
    53281 ataatcccgc ccatggtcgg cgtaccgcgc ttgctgaagt gtgattcagg accgtcgtta
    53341 cgcactacct gaccaaagga aagtttttgc aaatgagcaa tcatacgcgg gcccatccac
    53401 aatgagatga acagcgcggt cagcaggctg acgatggcgc gaaacgtcag ataggaaaag
    53461 acgttaaagc cggaataata tttgaccaaa tgttcggcca gccaaactaa catgtcccat
    53521 tctcctgtaa agcgcgtact acctcttcca tggcagcact acgtgaaccc ttaactaaaa
    53581 tcgtaattac ctgttgctca gcaatcagtg atttaagacg cgtaattaac gcagttttat
    53641 cagcaaaatg ttcgccaacg ccgctggcgg tgctgatagc atggctttgt ttacccatgc
    53701 ttaacacgcg gtcaatacca gcagcttttg ccgcctcacc cacctgtaca tggcaggctt
    53761 cgctttcagc gcccagttcc gccatatcgc ccaccaccag cacgcggtag cccggcattt
    53821 cagccagtac ctgaacagct gcggtcattg aaccgacatt ggcgttgtag gagtcgtcga
    53881 gcagcaactg gttttctgcc agttggatgg ggaacagacg gccaggaaca gctttcagat
    53941 ttgccagccc cgctttgata gcatcaagcg ttgcgcccac ggacatggag agcgcagcgg
    54001 ctgccagcgc attcgcaata ttgtgacgcc ccggcaacgg cagcagaaca tcgacgctac
    54061 cggttggggt ttgtagggta aattccatac cgtgcgaggt cacatggata ttggtggcgg
    54121 tgaaatcgct gttggcggca ttgggtgaga aacgccacac tttgcgtgag ccaattacgc
    54181 tctgccagtt cagccagtcg ttgttgtcgg cgttcataat ggcgataccg ttttccggca
    54241 ggccgctaaa gatttcacct ttcgctttcg cgacacccgc aagcgagcca aaaccttcca
    54301 gatgcgccgc tgccaggttg ttgaccagcg ccgcttccgg gcgagtcaga ctcacagtcc
    54361 aggcaatctc gccctgatgg ttcgcgccaa gttcaataac tgcgtaatcg tattccggcg
    54421 ttaagcgcaa cagcgtcatc ggtacaccga tgtcgttgtt gagattgcct gccgtataaa
    54481 gcgtgttgcc gcactggctt aaaatcgccg ccgtcatctc tttaacggag gttttgccgg
    54541 aggaccccgt cagagcaacc acgcgcgccg gaacttgctg gcgaacccat gcagccagtt
    54601 caccaaacgc cagacgcgta tccttgacga ttaactgcgg caggtcgatg tccagcggac
    54661 ggctaaccag tagtgcgcct gcgccgccag ctttcgcctg gtcggcaaaa tcgtgggcat
    54721 caaaacgttc acctttcagg gcaacaaaca ggcagcccgg cgtcagtttt cgggtatcag
    54781 tggttacagc atcaagggtg atatctgcac cttgcagttc accgttgaga atatcggtaa
    54841 gttggctaag ggttacgcta atcatgcaat cccccccagc agacgcgcca ccgtgacgcg
    54901 atcggagtag tccagacgct gattgccaac aatctggtaa tcctcatggc ctttgcccgc
    54961 gaccagtacc acatcattct ctttagcctg cataacggcg caagtcaccg cttcagcacg
    55021 gccttccatc actttggcat gtccggcatc taacattccc gccagaatat cgttgatgat
    55081 ggcacgcggt tcttcggtac gcgggttatc gtccgtcacc accgccacgt cagcaaactc
    55141 ttcggcaatt gcgcccatca gtggacgctt acctttatcg cgatcgccac cacagccaaa
    55201 gacgcaccac agcttgcccg cacagtgcag gcgcgccgcc tgtaaggctt tttccagtgc
    55261 atccggcgta tgcgcgtaat ccaccaccac cgtcggtttg cctggcgcag tgaacacttc
    55321 catacgtccg caaaccggtt gcagacgcgc ggcggttttc agcagatcag ccagtggata
    55381 gcccagcgcc agcagtgttg ccagcgcgag caacaggttg ctgacgttaa aagcgcccat
    55441 cagatggctt tcaatttcgc catcgcccca gcttgaacta aagcgaatgg tcgcgccgct
    55501 gtcgtgatag ttcacttcgg tcgctttcaa ccagcgtccg tgacagttcg gattgatatg
    55561 atcttccatt gataccgcaa ccgcgtccgg cagttttgcc agccagcggc ggcccacttc
    55621 atcgtcggcg ttaataatcg cctgaccgca atgatgttca gaataaagca gccatttcgc
    55681 agcttcgtag tgttccatat caccatgata atcaaggtga tcgcggctta agttggtaaa
    55741 gaccgacgcc ggaaatttca atgccgccac acggtgctgt accagcccgt gggaggaaac
    55801 ttccattgcg caaaaagtcg cgccctgatc caccagcccc gccagctcat gctgaacatc
    55861 aactgccgaa ccggtagtat tttctgtcgg gatcactttc cccagcagac cgttaccaac
    55921 ggtgcccatt accgcgctgg tttcgccaag cagttggctc cactgcgcca gaagctgggt
    55981 cgtcgtggtt ttgccgttgg tgcccgttac gcctacgaga cgtaaattgt cagagggttc
    56041 atggtaaaag cggcccgcca gtgcagataa acgctcgttg agctggctga gatagatgac
    56101 cggtacgccg tgcatttcac ggatttcacc gtcggtcgcc tcatctttcg cctctgcaat
    56161 aatggcagcc acaccttgcg ctatcgcctg cgggatatat cgacgcccgt ccgcctgatg
    56221 acctactaca gctacaaaga gatcgcccgc cgcagccaca cagctgtcga gtgtcatctc
    56281 tcgcagtgct cgcgaaggtg cgtctggcac ccacggagca agaaggtcgc gcaaattacg
    56341 atctgccacc tgtcccctcg ccttgattaa tcacaaattc atttttatcg cccgttgtca
    56401 gcgcatccgg ctcgatgttc atggtacgca atacgccgcc catgatggca ccaaagaccg
    56461 gcgcggaaac ggcgccgccg tagtatttac ccgcctgcgg atcgttgata acaacaacca
    56521 gcgcgaagcg cggctgactc gcaggcgcaa cgcctgcggt ataagcaata tatttattga
    56581 tgtagcgacc gtccggcccg acctttttcg cggtaccggt tttaatggcg atacgatagc
    56641 ctttaatcgc cgccttcacg ccgccgccgc ctggtagcgc cacgctttcc atcatatgca
    56701 ccacggtgcg aacaatggat tccgggaaga cacgttcacc gggaaccggg gggtcaactt
    56761 tggtaatcga cagtgggcga taaatgccgt agctgccgat agttgcgtag actcgcgcta
    56821 actgtaacgg tgttaccatt agcccgtagc cgaaagagaa ggtggccctc tctatgtcag
    56881 accaccgttg tttttgagga tataagccac tgcgttctcc gaccaacccc aaattggtcg
    56941 cttttcccag tccaaaacgt gagtaagtat ctactaacgc tgaggacggc atcgttaacg
    57001 ccagcttgga aacaccgacg ttactcgact tctgtaatac cccggtcagg gttaattcgc
    57061 tgtagcgtgc cacgtctttg atttcgtggc cgttaattcg ataaggaatg gtattgagta
    57121 ccgagttttc ccgcaccacg ccacgttgca acgcggtcat caccaccatc ggtttaaccg
    57181 ttgagcccgg ttcaaacacg tcggtgatgg tacggttacg catcgactct ttcggcgtgc
    57241 cgctcagatt gttagggttg tatgacgggc tgttagccat cgccagcact tcacctgtgt
    57301 tgacatccac cagcacggcg ctaccagatt cagccttgtt aaaggccacc gcgttgttca
    57361 gttcgcgata aaccagcgcc tgcaggcgtt catcaatact cagcgccagg ttgtgcgctg
    57421 cctggctgtc agtagaagaa atatcttcaa ttacgcgacc atagcggtct ttacgcacaa
    57481 tgcgctcacc cggctgcccg gtaagccatt tatcgaaact cttctcaacg ccctcaatcc
    57541 cttgactatc gacgttagta aagccgatga ggtgagcagt cacttcgccg gacggatagt
    57601 aacggcgaga ctcttcacgc agatgaatcc ccggcagttt cagttttttg atgtagtccg
    57661 ccatgtcaga gtttacctga cgcgccagat aaataaagcg ccctttcggg ttggcgttaa
    57721 tgcgggcaga aagctgatcc agcggaatat tgagcgcgtt agccagcgcc ttccagcggt
    57781 caccgacgct gataccgcca gcgtcatgca cttctttcgg gtcagcccaa atcgctttta
    57841 ccggcacgct caccgctaac gggcgaccag aacggtcagt aatcatgccg cgggaggtgg
    57901 aaacttgctg aacgcgaaga gaacgcatgt cgccctcttt caccagcata tccggggaga
    57961 taacttgtaa ccacgctacg cgtccgagca gaaaagccag cgccaggaga atacagccgc
    58021 ataacaacgc aaaacgccaa ctgataaagt tggcatgttc ttcctgacgt tttggtttct
    58081 gcgttttcgc cgctgctttc atgcgtcgcg tttatcctta tttttgcact acgatatttt
    58141 cttgtgacgg atcaacatgc tgcatttgca gcttttccgt ggcgatcctt tccacccggc
    58201 tatggtcgcc gagcgcattc tcttcaagga tcaggttgcg ccattcaatg tctaaagcat
    58261 ctcgctccag caccagttgt tcgcgctgag cggtcagtaa acgggtatgg tgcgccgtgg
    58321 ttaccacggt caccgccgtc aaaataatgc aaatgaacag gcagagtggc agcttcccaa
    58381 atcgcaaaag atcgtcaccg ataacaccag gcaatgcatg gcgctcgtgg cttcccatcg
    58441 atcctttaac tttgcttaga gcttctgtca ctctgctgat catgcgttcg tcctctctgc
    58501 aatacgcaga actgaactac gggcacgagg gttctcagcc acctcttctt cgcccggcat
    58561 taacttgcct agtgctcgca gctgacggcc acccagtttt ttgagctgct cttcagtcat
    58621 cggtaaccct gccggaactt gcggaccgcg gctgttttca cgcataaaac gtttcacaat
    58681 acggtcttcc agcgagtgga agctgatgat cgaaagccgc ccacccgggg ccagcacgtt
    58741 gagcgagctt tttagcgcct gctctatctc ctccagttca ctgtttaccc aaatgcgcac
    58801 cgcctggaag gtacgggtcg cgggatgttt aaacttatct ttcaccggcg ttgcagcagc
    58861 cacgacttcc gccagttctt tggtgcgggt catcggctgt tcgcggttac gctcgacaat
    58921 ggcgcgggca atgcgtttgg caaaatgctc ttcaccatag gttttcaata cccaggcgat
    58981 atcggcttct tctgcggttt gtagccattc agcggctgac tgcccacggg ttgggtccat
    59041 acgcatgtcc agcggaccat cgcgcataaa ggaaaagccg cgttcagcat catcaagttg
    59101 cggtgaagag acgccaagat cgaggagaat gccgtcgatc ttgccaataa gatcgcgctc
    59161 ggcaacgtat tcgcccagcg cggagaaagg tccgtggatg atggagaagc gcggatcatc
    59221 aatagtcttc gcaacggcga tagcctgcgg gtcgcgatcg atcgccagca aacgcccctc
    59281 ttcgccaagc tgcgagagga tcagacgtga gtgaccaccg cgaccaaaag tcccatcaat
    59341 gtagatgcca tcaggacgga tattgaggcc attaacggct tcatccagca gcaccgtagt
    59401 atgtttatag ttttccatca ttttatagag acaagtcctg cagtcgctcc gataagtctc
    59461 cggtagccaa ctgctctgcg tcgatatctt ccttgacctg ttgatgccag gttgtttcat
    59521 cccacagctc aaacttgttg aactgtccaa ccagcatcac ttcttttgtc agcccggcat
    59581 gttgccgcag tactggcgcg attaacaatc gacctgcgcc atccatctga cattcgctgg
    59641 catgacctaa cagtaggcgc tgcacacggc gctcaaccgg gttcatgctc aacagacgcg
    59701 ataatttttg ctcgataatt tcccattcag gcagggggta aagcagcagg cacgggtgat
    59761 gaatgtcaat ggtgcaaacc atttgaccgg cagcgttctc aagcagctgt tcccgataac
    59821 gggtaggcac tgataagcgc cctttgctgt cgagattgac taacgttgct ccccggaaca
    59881 tgccagcctc accccttatt cccactttgc cccacaattt cccactgaaa ggagtttacg
    59941 gagcggagga aaagcttgtc aagccagtca caaggcatac cgaggtaaaa aacccgatgt
    60001 ttacggctaa tatcagcgtt gtttatttta cgtccaacgt gcgaagcaaa ttaacgttat
    60061 gaatagctgt aagaaaaaat ccctcatcgt catcactgtt ttaaaatgac tcactatttt
    60121 ccacagcaaa tataaagtgt cagtttgcga cgcgagcggc attttaggac atatcttccc
    60181 cggttaacag tccttgttac gtctgtgtgg cgcttgatgc cagcgggttt gcgcaaaagc
    60241 aaagaaaagt gtttgttaaa tcggcaattc atcgccgcat gtaacaaaat aatgcaaaaa
    60301 cgcgctgtcg atatcactta attattgtga atttcagctg gtgagttaat taacgggcgg
    60361 gtttaaaacc atttatcaat aatagtcagg aatagtctta tttactttaa ggatattgat
//
LOCUS       DAJTQY010000012        59908 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.012, whole genome shotgun sequence.
ACCESSION   DAJTQY010000012 DAJTQY010000000
VERSION     DAJTQY010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 59908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59908
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.012"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            533..1822
                     /gene="sdaC"
                     /locus_tag="OO349_000771"
     CDS             533..1822
                     /gene="sdaC"
                     /locus_tag="OO349_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HCR5865950.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            1880..3247
                     /gene="sdaB"
                     /locus_tag="OO349_000772"
     CDS             1880..3247
                     /gene="sdaB"
                     /locus_tag="OO349_000772"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HCR5865951.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSLDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            3358..4113
                     /gene="xni"
                     /locus_tag="OO349_000773"
     CDS             3358..4113
                     /gene="xni"
                     /locus_tag="OO349_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HCR5865952.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKADRPPMPEELHDEMPALRAAFEQRGVPCWSASGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(4166..5314)
                     /gene="fucO"
                     /locus_tag="OO349_000774"
     CDS             complement(4166..5314)
                     /gene="fucO"
                     /locus_tag="OO349_000774"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HCR5865953.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEGARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDISALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(5342..5989)
                     /gene="fucA"
                     /locus_tag="OO349_000775"
     CDS             complement(5342..5989)
                     /gene="fucA"
                     /locus_tag="OO349_000775"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HCR5865954.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            6536..7852
                     /gene="fucP"
                     /locus_tag="OO349_000776"
     CDS             6536..7852
                     /gene="fucP"
                     /locus_tag="OO349_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HCR5865955.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSSHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            7885..9660
                     /gene="fucI"
                     /locus_tag="OO349_000777"
     CDS             7885..9660
                     /gene="fucI"
                     /locus_tag="OO349_000777"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HCR5865956.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYDEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATKWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            9769..11188
                     /gene="fucK"
                     /locus_tag="OO349_000778"
                     /pseudo
     CDS             9769..11188
                     /gene="fucK"
                     /locus_tag="OO349_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
     gene            11190..11612
                     /gene="fucU"
                     /locus_tag="OO349_000779"
     CDS             11190..11612
                     /gene="fucU"
                     /locus_tag="OO349_000779"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HCR5865957.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMEPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPLLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            11670..12401
                     /gene="fucR"
                     /locus_tag="OO349_000780"
     CDS             11670..12401
                     /gene="fucR"
                     /locus_tag="OO349_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HCR5865958.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(12445..13545)
                     /gene="rlmM"
                     /locus_tag="OO349_000781"
     CDS             complement(12445..13545)
                     /gene="rlmM"
                     /locus_tag="OO349_000781"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HCR5865959.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QTQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(13538..13933)
                     /locus_tag="OO349_000782"
     CDS             complement(13538..13933)
                     /locus_tag="OO349_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HCR5865960.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            complement(13952..14869)
                     /gene="gcvA"
                     /locus_tag="OO349_000783"
     CDS             complement(13952..14869)
                     /gene="gcvA"
                     /locus_tag="OO349_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HCR5865961.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGFNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            complement(15220..15447)
                     /locus_tag="OO349_000784"
     CDS             complement(15220..15447)
                     /locus_tag="OO349_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HCR5865962.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDIGMISYKDANGNKQQINRTDVKEMVELEQ"
     gene            15639..16844
                     /gene="csdA"
                     /locus_tag="OO349_000785"
     CDS             15639..16844
                     /gene="csdA"
                     /locus_tag="OO349_000785"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HCR5865963.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTRAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEEALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            16844..17287
                     /gene="csdE"
                     /locus_tag="OO349_000786"
     CDS             16844..17287
                     /gene="csdE"
                     /locus_tag="OO349_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HCR5865964.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGNSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(17338..18144)
                     /gene="tcdA"
                     /locus_tag="OO349_000787"
     CDS             complement(17338..18144)
                     /gene="tcdA"
                     /locus_tag="OO349_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HCR5865965.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(18383..19480)
                     /gene="mltA"
                     /locus_tag="OO349_000788"
     CDS             complement(18383..19480)
                     /gene="mltA"
                     /locus_tag="OO349_000788"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HCR5865966.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LGDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            19689..19765
                     /locus_tag="OO349_000789"
     tRNA            19689..19765
                     /locus_tag="OO349_000789"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19723..19725,aa:Met,seq:cat)
     gene            19799..19875
                     /locus_tag="OO349_000790"
     tRNA            19799..19875
                     /locus_tag="OO349_000790"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19833..19835,aa:Met,seq:cat)
     gene            complement(19950..21203)
                     /gene="amiC"
                     /locus_tag="OO349_000791"
     CDS             complement(19950..21203)
                     /gene="amiC"
                     /locus_tag="OO349_000791"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HCR5865967.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEEEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            21435..22766
                     /gene="argA"
                     /locus_tag="OO349_000792"
     CDS             21435..22766
                     /gene="argA"
                     /locus_tag="OO349_000792"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HCR5865968.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     LHRQLESGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSLHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(22828..24654)
                     /gene="recD"
                     /locus_tag="OO349_000793"
     CDS             complement(22828..24654)
                     /gene="recD"
                     /locus_tag="OO349_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HCR5865969.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASNPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHTIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(24654..28196)
                     /gene="recB"
                     /locus_tag="OO349_000794"
     CDS             complement(24654..28196)
                     /gene="recB"
                     /locus_tag="OO349_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HCR5865970.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLIVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEPPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLLITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDTFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDLHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(28189..31077)
                     /gene="ptrA"
                     /locus_tag="OO349_000795"
     CDS             complement(28189..31077)
                     /gene="ptrA"
                     /locus_tag="OO349_000795"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HCR5865971.1"
                     /translation="MPRSTWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMINANGYTQRLPQLFQALLEGY
                     FSYTATEEQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(31253..34621)
                     /gene="recC"
                     /locus_tag="OO349_000796"
     CDS             complement(31253..34621)
                     /gene="recC"
                     /locus_tag="OO349_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HCR5865972.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNSLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEAPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FHTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(34634..34966)
                     /locus_tag="OO349_000797"
     CDS             complement(34634..34966)
                     /locus_tag="OO349_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCR5865973.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVLMVMIVTALSGIQRTSMNSL
                     ASRNQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMT
                     RLHCPNRQ"
     gene            complement(34951..35358)
                     /locus_tag="OO349_000798"
     CDS             complement(34951..35358)
                     /locus_tag="OO349_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HCR5865974.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDAQVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGKIVFSPRGWSDFCPLKEGALCQLP"
     gene            complement(35355..35918)
                     /locus_tag="OO349_000799"
     CDS             complement(35355..35918)
                     /locus_tag="OO349_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HCR5865975.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(35909..36379)
                     /locus_tag="OO349_000800"
                     /pseudo
     CDS             complement(35909..36379)
                     /locus_tag="OO349_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
     gene            complement(36563..37357)
                     /gene="thyA"
                     /locus_tag="OO349_000801"
     CDS             complement(36563..37357)
                     /gene="thyA"
                     /locus_tag="OO349_000801"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCR5865976.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGVKAPVAI"
     gene            complement(37364..38239)
                     /gene="lgt"
                     /locus_tag="OO349_000802"
     CDS             complement(37364..38239)
                     /gene="lgt"
                     /locus_tag="OO349_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HCR5865977.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(38390..40636)
                     /gene="ptsP"
                     /locus_tag="OO349_000803"
     CDS             complement(38390..40636)
                     /gene="ptsP"
                     /locus_tag="OO349_000803"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR5865978.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGSANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSCIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(40649..41179)
                     /gene="rppH"
                     /locus_tag="OO349_000804"
     CDS             complement(40649..41179)
                     /gene="rppH"
                     /locus_tag="OO349_000804"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HCR5865979.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            41864..42553
                     /gene="mutH"
                     /locus_tag="OO349_000805"
     CDS             41864..42553
                     /gene="mutH"
                     /locus_tag="OO349_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HCR5865980.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            42622..43335
                     /locus_tag="OO349_000806"
     CDS             42622..43335
                     /locus_tag="OO349_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCR5865981.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASISWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            43473..43691
                     /gene="ygdR"
                     /locus_tag="OO349_000807"
     CDS             43473..43691
                     /gene="ygdR"
                     /locus_tag="OO349_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HCR5865982.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            43799..44839
                     /locus_tag="OO349_000808"
     CDS             43799..44839
                     /locus_tag="OO349_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HCR5865983.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRCQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(44872..46062)
                     /gene="lplT"
                     /locus_tag="OO349_000809"
     CDS             complement(44872..46062)
                     /gene="lplT"
                     /locus_tag="OO349_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HCR5865984.1"
                     /translation="MSESVHINTSLWSKGMKAVIVAQFLSAFGDNALLFATLTLLKAQ
                     FYPEWSQPILQMVFVGAYILLAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASAIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAARPGQSWNLINMTRSFLNACTSLWCNGETR
                     FSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVVIGIVVGAGAAAKLV
                     TLETVSRCMPAGILIGVVVPIFSLQHELLPAYALLMLIGVLGGFFVVPLNALLQERGK
                     KSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWIW
                     QRRH"
     gene            complement(46055..48214)
                     /gene="aas"
                     /locus_tag="OO349_000810"
     CDS             complement(46055..48214)
                     /gene="aas"
                     /locus_tag="OO349_000810"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HCR5865985.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPDL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            48800..49831
                     /gene="galR"
                     /locus_tag="OO349_000811"
     CDS             48800..49831
                     /gene="galR"
                     /locus_tag="OO349_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HCR5865986.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(49838..51100)
                     /gene="lysA"
                     /locus_tag="OO349_000812"
     CDS             complement(49838..51100)
                     /gene="lysA"
                     /locus_tag="OO349_000812"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HCR5865987.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFEVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDTLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            51222..52157
                     /gene="lysR"
                     /locus_tag="OO349_000813"
     CDS             51222..52157
                     /gene="lysR"
                     /locus_tag="OO349_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HCR5865988.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPTGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGISVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSGHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(52144..52836)
                     /gene="ygeA"
                     /locus_tag="OO349_000814"
     CDS             complement(52144..52836)
                     /gene="ygeA"
                     /locus_tag="OO349_000814"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HCR5865989.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQMLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(52965..54383)
                     /gene="araE"
                     /locus_tag="OO349_000815"
     CDS             complement(52965..54383)
                     /gene="araE"
                     /locus_tag="OO349_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HCR5865990.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGGLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGAAFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(54698..55459)
                     /gene="kduD"
                     /locus_tag="OO349_000816"
     CDS             complement(54698..55459)
                     /gene="kduD"
                     /locus_tag="OO349_000816"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HCR5865991.1"
                     /translation="MILSAFSLQGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTAQGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYAIAVDGGWLAR"
     gene            complement(55489..56325)
                     /gene="kduI"
                     /locus_tag="OO349_000817"
     CDS             complement(55489..56325)
                     /gene="kduI"
                     /locus_tag="OO349_000817"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HCR5865992.1"
                     /translation="MDVRQSIHGAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFNDMDHVGVKD
                     LR"
     gene            complement(56612..57793)
                     /locus_tag="OO349_000818"
     CDS             complement(56612..57793)
                     /locus_tag="OO349_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HCR5865993.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            58048..59277
                     /locus_tag="OO349_000819"
                     /pseudo
     CDS             58048..59277
                     /locus_tag="OO349_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
BASE COUNT        14794 a        16067 c        14589 g        14458 t
ORIGIN      
        1 ttgccgggtt ttctcgtttt tggtcactta ctcatcaact catttcattt gttatatgaa
       61 tgtttcttac caccctcacg cgacaaatat catcacagtt aatatgtcat acaatttatg
      121 ttgcaacaca aacgtttccc tattttcata aaccgtattt tatcgctatg aaaaagaaat
      181 tatcgccatg attaactaaa agtattgatt ttttcagttc aacctacata tattgcgcgc
      241 cccggaagaa gtcagatgtc gtttaatggg caaatattgc ccttaaattc tcttttactt
      301 ttgatttaca gagtaaagcg ttgggataat ctatcttcca agcagattat tgtatttggg
      361 atcaagatca ctgatagata cataacttgt gtgtatcttt ccgccctcaa attattacgg
      421 cggtaaatga ttaagccatc gccgatagac agatttcatt tttacggtca ggcaccttcc
      481 ctggctgaac tggctaaaag ctgaattatt tgcattcctc caggagaaat agatggaaac
      541 gactcaaacc agcacgattg cgtcgaaaga ctctcgtagt gcctggcgca agacagacac
      601 catgtggatg ctgggccttt acggcacggc aatcggcgcg ggcgtgctgt tcctgccaat
      661 caacgccggt gttggcggta tgatcccgct gatcatcatg gctatccttg cgttcccgat
      721 gacgtttttt gctcaccgcg gcctgactcg cttcgtactg tctggtaaaa acccgggcga
      781 agacatcacc gaggttgtag aagaacactt tggtattggc gcaggtaaac tgattaccct
      841 gctctacttc ttcgctatct acccgatcct gctggtttat agcgtggcaa tcaccaatac
      901 cgttgaaagc ttcatgtctc accagctggg tatgacgcca ccgccgcgtg cgattctgtc
      961 gctgatcctg atcgtgggta tgatgaccat cgttcgcttt ggtgagcaga tgatcgttaa
     1021 agcgatgagt attctggtat tcccgtttgt tggcgtactg atgctgctgg ctctgtacct
     1081 gatcccgcag tggaacggcg cagcactgga aacgctgtct ctggacactg catctgcaac
     1141 cggaaacggt ctgtggatga ccctgtggct ggcaattccg gtaatggtgt tctcgttcaa
     1201 ccactctccg atcatctctt ctttcgccgt tgcgaagcgt gaagagtacg gcgatatggc
     1261 agaacagaaa tgctcgaaga tcctggcatt cgcacacatc atgatggtgc tgaccgtaat
     1321 gttcttcgtc ttcagctgcg tactgagcct gactccggca gacctggctg cggctaaaga
     1381 gcagaacatc tcgattctgt cttacctggc taaccacttt aacgcaccgg ttatcgcgtg
     1441 gatggctccg attatcgcga ttatcgctat caccaaatcc ttcctcggcc actacctggg
     1501 cgcacgtgaa ggcttcaacg gtatggtgat taaatctctg cgtggtaaag gtaagtctat
     1561 cgaaatcaac aagctgaacc gtatcactgc gctgttcatg ctggtaacga cctggattgt
     1621 tgccaccctg aacccgagca tcctgggtat gattgaaacc ctgggcggcc caatcatcgc
     1681 gatgatcctg ttcctgatgc cgatgtacgc aattcagaaa gtaccggcaa tgcgtaagta
     1741 cagcggtcac atcagcaacg tattcgttgt cgtgatgggt ctgattgcaa tctccgcaat
     1801 cttctactct ctgttcagct aagtcctttc gcgccgcttt cgggcggcgc ttcctccgtt
     1861 ttaacgcgat gtatttccta tgattagcgt attcgatatt ttcaaaatcg gcattggccc
     1921 ttccagttct cataccgttg gaccaatgaa agcgggtaaa caatttaccg acgatctgat
     1981 tgcccgtaac ctgcttaaag acgtgacccg cgtggtggtt gacgtgtacg gctcgctctc
     2041 tctgactggt aaaggccacc acacagatat cgccattatt atgggcctgg cgggtaacct
     2101 gccggatacc gtggatatcg attccatccc cggttttatt caggatgtga atactcatgg
     2161 tcgcctgatg ctggcaaacg gtcagcatga agtggagttc ccggttgatc agtgcatgaa
     2221 cttccatgca gataaccttt ctctgcatga aaacggtatg cgcattaccg cgctggcggg
     2281 cgataaagtc gtttacagcc agacttacta ctctattggc ggtggcttta tcgttgatga
     2341 agagcatttt ggccagcagg atagcgcacc ggttgaagtt ccttatccgt acagttcagc
     2401 agccgatctg caaaaacatt gtcaggaaac cgggctgtca ctctctggcc tgatgatgaa
     2461 aaacgagctg gcgctgcaca gtaaagaaga gctggaacag cacctggcga acgtctggga
     2521 agtcatgcgt ggcggtattg agcgcggtat ttccaccgaa ggcgtgttgc ctggcaaact
     2581 gcgcgttcca cgccgtgctg cggcactacg ccggatgctg gtcagcctgg ataaaaccac
     2641 cactgacccg atggcggttg ttgactggat caacatgttt gcactggcag tgaacgaaga
     2701 gaacgctgct ggcggtcgcg tggtgactgc gccgactaac ggtgcgtgcg ggattatccc
     2761 ggctgtgctg gcgtactacg ataagtttat ccgcgaagtg aacgctaact cactggctcg
     2821 ttacctgctg gtagccagcg ccattggttc tctttataag atgaacgcgt cgatttctgg
     2881 tgccgaagtg ggttgccagg gtgaagttgg cgtggcgtgc tctatggcgg cagctggtct
     2941 ggcagaactg ttaggcgcaa gcccggcgca ggtttgcatc gcagcggaaa tcgcgatgga
     3001 gcacaacctc ggtctgactt gtgacccggt cgcggggcag gttcaggtac cgtgcattga
     3061 gcgtaacgcc attgcagcag tgaaagcggt gaacgccgcg cgtatggcac tgcgccgtac
     3121 cagcgaaccg cgcgtctgcc tcgataaagt tatcgaaacc atgtacgaaa caggtaaaga
     3181 catgaacgcc aagtaccgcg aaacctctcg cggcggcctg gcgatgaaga tcgttgcctg
     3241 cgattaatct ctcccagagg cctcgttttg cgaagcctct tcctgttttc tcaaccagcc
     3301 atagcctgtt ccggcatcga atgttaccct tatcgcctga tctttaaggg ggttatcgtg
     3361 gctgttcatt tgcttattgt cgatgcactg aatcttatcc gtcgcattca tgccgttcag
     3421 ggttcgccct gtgtcgaaac ctgccagcat gcgctcgatc agctcattat gcacagccag
     3481 ccaacccacg cggtcgccgt ttttgatgat gaaaaccgca gtagcggctg gcgtcatcag
     3541 cgtttaccag attacaaagc ggatcgaccg ccaatgccgg aagagttgca cgacgagatg
     3601 cctgcattac gcgccgcctt tgagcaacgc ggcgtcccgt gctggtcagc cagcggcaac
     3661 gaagccgatg acttagccgc cacactggcg gtcaaagtga cacaggccgg gcatcaggca
     3721 acgattgttt cgacagataa aggctactgt cagttacttt caccgacatt acgtattcgt
     3781 gattacttcc agaaacgttg gctggatgcg ccatttatcg ataaagaatt tggcgttcaa
     3841 ccgcagcagt tgcccgatta ctggggactt gcggggatca gcagttcaaa ggtaccgggt
     3901 gttgcgggaa tcggaccaaa aagcgccacg cagctgctgg tcgagtttca gagtctggaa
     3961 gggatatatg agaatctgga tgcggttgcc gaaaagtggc gcaaaaaatt agaaacccat
     4021 aaagagatgg cgtttctgtg ccgcgatatt gcccgcttac aaaccgattt gcatatcgac
     4081 ggcaatttac agcaattgcg gttggtacgg taacggcgag ccggatacgc cgcaaatcgt
     4141 atccggcatt atcacatcag cgcatttacc aggcggtatg gtaaagctct acaatatcct
     4201 caagcgttgc ttcacgcggg ttgccaccgg tacaaacatc atccagtgcc gcctgagcca
     4261 gtgccgaaat gtcttccttg cgtacaccaa catcacgcaa atgtggcgga ataccgacat
     4321 cacggttgag ggcaaacacc gcttcaacag cggcattacg cgccccttcc aggctcatac
     4381 cttccacttt cacgcccata acgcgcgcga tatcgcggta cttctcaccg gtaaagtcag
     4441 cgttatagcg catgacatgc ggtaacagga tggcgttcgc aacaccgtgt ggagtgttat
     4501 aaaacgcgcc cagtggatgc gccataccat gcaccaaccc taacccaaca ttcgagaagc
     4561 ccatacccgc aacatactgc ccgagcgcca tttcttctcc ggcatcctta tcaccagcaa
     4621 ccgatcctcg cagcgcccca gcaatgattt caatcgcttt aatgtgcagt gcatcggtta
     4681 gtgcccacgc gccacgggta atatacccct caatagcatg agtgagcgca tcaacacccg
     4741 tcgcggcttt cagcgccgga ggcataccat ccatcatgtc agcatcaata aacgccacct
     4801 gcgggatatc atgcggatca acgcaaacaa acttgcgccg tttttcttcg tcagtgatca
     4861 cgtagttaat ggtcacttct gccgcagtac ctgctgtggt ggggattgcc agaatcggta
     4921 cactgggttt attggtcggg gaaagccctt ccaggctacg cacatcggca aactccgggt
     4981 tgttgctgat aatgccaatt gctttacaag tatcctgtgg agaaccacca ccaatagcga
     5041 tcaggtaatc cgcgccgcta ttctggaata caccgagccc ttctttgacg acagtaattg
     5101 ttgggttggg cactacgccg tcgtaaatcg cccatgccag ccctgcagca tccatcttat
     5161 cggtcacttt cgccaccacg ccgcattgca ccagcgtttt atcggtgacg atcagcgcct
     5221 tctgataacc acggcgtttc acctcatcgg ttaaagcccc aacagcaccc cgaccaaacc
     5281 atgccgtttc gttcagaatc attctgttag ccatcatcct tctccttgtt gctttacgaa
     5341 attactcttc aatccgtaac ccataggttt tgaatttctc cagcactacg gcaatctctt
     5401 catcgctcag cactggcacc gggtccgtaa tcgccagggt cgtcaggtaa agttgcgcca
     5461 gcacttcaac ttcatgcgcc agccataacg ctttttccag attcacctca caagcgataa
     5521 gcccatgatg ttgtaacaaa gttgccttac gatttttgag agccagcgca acatgttcag
     5581 aaagttcgcg tgttccaaag gtcgcataag gcgcgcaagg aatagaatta ccgccagccg
     5641 ccgcaatcat gtagtgaata gcggggatcg atcggttaag aatggaaact gccgtgcaat
     5701 gaacggcatg attgtgaaca accgcgttgg catccggtct gctttgatag gctgccatat
     5761 ggaaacgcca ttcgcttgag gggagctttc cttcctcatg tttaccgttg ccatcaataa
     5821 agacaatatg cgactccgtc agtttttcat atggaatgcc tgtaggcgta atcagcatcc
     5881 catcctgata acgtacactg acgttccccg ctgtcccctg gttcagtccc aggcgggtca
     5941 tttccaggca agtgtcaata atctgacgag caagtttatt tcgttccatt agctacctct
     6001 ctctgattca aaacagggca ataatgttgt tcctttcaca ctattgaatt agccgtttaa
     6061 ttacccacca tcttcttcct gattaacaag aaagaaattc acaagcttat attttgtgac
     6121 ctagctcaac taatcacagt aaataactgc aagttctctt tttataaccc cgttaaaaat
     6181 gaccgctctt aaaaatattt atcaaaacgg tcatttttcc atttcccctc gccagaaatg
     6241 cccgttttcg gcacaaacaa ttaatacggt catctggttt gtgtttttta tgatttattt
     6301 tctgaaacgg gcatgaaatt tcgattatta aagtgatggt agtcacataa agtcaccttc
     6361 cagctaataa gtgtgaccgc cgtcatatta cagagcgttt tttatttgaa aatgaatcca
     6421 tgagttcatt tcagacaggc aaatattcac cgatatgaag cccgaactcg ctggttttgc
     6481 acttttgaaa acataaccga ttacgtgctt aagcttctga acctaagagg atgctatggg
     6541 aaacacatca atacaaacgc agagttaccg tgcggtagat aaagatgcag ggcaaagcag
     6601 aagttacatt attccattcg cgctgctgtg ctcactgttt tttctttggg cggtagccaa
     6661 taaccttaac gacattttat tacctcaatt ccagcaggct tttacgctga caaatttcca
     6721 ggctggcctg atccaatcgg ccttttactt tggttatttc attatcccaa tccctgctgg
     6781 gatattgatg aaaaaactca gttataaagc agggattatt accggattat ttttatatgc
     6841 cttgggcgct gcattattct ggcccgctgc agaaataatg aactacacct tatttttagt
     6901 tggcctattt attattgcag ccggattagg ttgtctggaa actgccgcaa acccttttgt
     6961 tacggtatta gggccggaaa gcagtagtca cttccgctta aatcttgcgc aaacatttaa
     7021 ctcgtttggc gcaattatcg cggttgtctt tgggcaaagt cttattttgt ctaacgtgcc
     7081 acatcaatcg caagacgttc tcgataaaat gtctccagag caattgagtg cgtataaaca
     7141 cagcctggta ttatcggtac agacacctta tatgatcatc gtggctatcg tgttactggt
     7201 cgccctgctg atcatgctga cgaaattccc ggcattgcag agtgataatc acagtgacgc
     7261 caaacaagga tcgttctccg catcgctttc tcgtctggcg cgtattcgtc actggcgctg
     7321 ggcggtatta gcgcaattct gctatgtcgg cgcacaaacg gcctgctgga gctatttgat
     7381 tcgctacgct gtagaagaaa ttccaggtat gactgcaggc tttgccgcta actatttaac
     7441 cggaaccatg gtgtgcttct ttattggtcg tttcaccggt acctggctca tcagtcgctt
     7501 cgcaccacac aaagtcctgg ccgcctacgc attaatcgct atggcactgt gcctgatctc
     7561 agccttcgct ggcggtcatg tgggcttaat agccctgact ttatgcagcg cctttatgtc
     7621 gattcagtac ccaacaatct tctcgctggg cattaagaat ctcggccagg acaccaaata
     7681 cggttcgtcc ttcatcgtta tgaccattat tggcggcggt attgtcactc cggtcatggg
     7741 ttttgtcagt gacgcggcgg gcaacatccc cactgctgaa ctgatccccg cactctgctt
     7801 cgcggtcatc tttatctttg cccgtttccg ttctcaaacg gcaactaact gaacatattt
     7861 tccgaataaa gtgaggaatc tgtaatgaaa aaaatcagct taccgaaaat tggtatccgc
     7921 ccggttattg acggtcgtcg catgggtgtt cgtgagtcgc ttgaagaaca aacaatgaat
     7981 atggcgaaag ctacggccgc actgctgacc gagaaactgc gccatgcctg cggagctgcc
     8041 gtcgagtgtg tcatttccga tacctgtatc gcgggtatgg ctgaagccgc tgcttgcgaa
     8101 gaaaaattca gcagccagaa tgtaggcctc accattacgg taacgccttg ctggtgctat
     8161 ggcagtgaaa ccatcgacat ggatccaacc cgcccgaaag ccatttgggg ctttaacggc
     8221 actgaacgcc ccggcgctgt ttacctggca gcggctctgg cagctcacag ccagaaaggc
     8281 atcccggcat tctccattta cggtcatgac gttcaggatg ccgatgacac atcgattcct
     8341 gccgatgttg aagaaaaact gctgcgcttt gcccgcgccg gtttggccgt cgccagcatg
     8401 aaaggtaaaa gctatctgtc gctgggcggc gtttcgatgg gtatcgccgg ttctattgtt
     8461 gatcacaact tctttgaatc ctggctggga atgaaagtcc aggcggtgga tatgaccgaa
     8521 ctgcgtcgcc gtatcgatca gaagatttac gacgaagccg aactggaaat ggcactggcc
     8581 tgggctgata aaaacttccg ctatgacgaa gatgaaaata acaaacagta tcaacgcaat
     8641 gccgagcaaa gccgcgcagt tctgcgcgaa agtttactga tggcgatgtg tatccgcgac
     8701 atgatgcaag gcaacagcaa actggccgat attggtcgcg tggaagaatc acttggctac
     8761 aacgccatcg ctgcgggctt ccaggggcaa cgtcactgga ccgatcaata tcccaatggt
     8821 gacaccgccg aagcgatcct caacagttca tttgactgga atggcgtgcg cgaacccttt
     8881 gtcgtggcga ccgaaaacga cagtcttaac ggcgtggcaa tgctaatggg tcaccagctc
     8941 accggcaccg ctcaggtatt tgccgatgtg cgtacctact ggtcaccaga agcaattgag
     9001 cgtgtaacgg ggcataaact ggacggactg gcagaacacg gcatcatcca tttgatcaac
     9061 tcaggttctg ctgcgctgga cggttcctgt aaacaacgcg acagcgaagg taacccgacg
     9121 atgaagccgc actgggaaat ctctcagcaa gaggctgacg cttgcctcgc cgctaccaaa
     9181 tggtgcccgg cgatccacga atacttccgt ggcggcggtt actcttcccg cttccttacc
     9241 gaaggcggcg tcccgttcac catgactcgt gtcaacatca tcaaaggcct gggaccggta
     9301 ctgcaaatcg cggaaggctg gagcgtggaa ttgccgaagg atgtgcatga catcctcaac
     9361 aaacgcacca actcaacctg gccaaccacc tggtttgcac cgcgcctcac tggtaaaggg
     9421 ccgtttacgg atgtgtattc ggtaatggcg aactggggtg ccaaccacgg agttctgacc
     9481 atcggccacg ttggcgcaga ctttatcacc cttgcctcca tgctgcgtat cccggtatgt
     9541 atgcacaacg ttgaagagac caaagtgtat cgtccttctg cctgggctgc gcacggcatg
     9601 gatattgaag gccaggatta ccgcgcttgc cagaactacg gtccgttgta caagcgttaa
     9661 tacattacac cctgatgtga tgcccggtcg ctccggctac cgggcctgaa caagcaagag
     9721 tggttagccg gataaacaat gttatccggc tatattgcag gagcgattat gaaacaagaa
     9781 gttatcctgg tactcgactg tggcgcgacc aatgtcaggg ccatcgcggt taatcggcag
     9841 ggaaaaattg ttgcccgcgc ctcaacgcct aatgccagcg atatcgcgat ggaaaacaac
     9901 acctggcacc agtggtcttt agactgccat tttgcaacgc tttgctgatt gctgtcggca
     9961 aatcaatagt gaactgactg aatgccacat ccgcggtatc gccgtcacca cctttagtgt
    10021 ggatggcgct ctggtagata agcaaggcaa tctgctctat ccgatcatta gctggaaatg
    10081 tccgcgaaca gcagcggtta tggacaatat tgaacggtta atctccgcac agcggttgca
    10141 ggctatttct ggcgtcggag cctttagttt caatacgtta tataagttgg tgtggttgaa
    10201 agaaaatcat ccacaactgc tggaacgcgc gcacgcctgg ctcttcattt cgtcgctgat
    10261 taaccaccgt ttaaccagcg aattcactac tgatatcacg atggccggaa ccagccagat
    10321 gctggatatc cagcaacgcg atttcagtcc gcaaatttta caagccaccg gtattccacg
    10381 ccgactcttc cctcgtctgg tggaagcggg tgaacagatt ggtacgctac agaacaacgc
    10441 cgcagcaatg ctcggcttac ccgtcggcat accggtgatt tccgcaggtc acgataccca
    10501 gttcgccctt tttggcgctg gtgccgaaca aaatgaaccc gtgctctcct ccggtacatg
    10561 ggaaatttta atggttcgca gcgcctaggt tgatacttcg ctgttaagtc agtacgccgg
    10621 ttccacctgc gaactagata gccaggcatg gttgtataac ccaggtatgc aatggctggc
    10681 atccggcgtg ctggaatggg tgagaaaact gttctggacg gctgaaacac cctggcaaat
    10741 gttgattgaa gaagctcgtc tgatcgcacc tggcgcggat ggtgtgaaaa tgcagtgtga
    10801 tttattgtcg tgtcagaacg ctggctggca aggagtgacg cttaatacca cgcgggggca
    10861 tttctatcgc gcggcgctgg aagggttaac tgcgcaatta cagcgcaatc tacagatgct
    10921 ggaaaaaatc gggcacttta aagcctatga attattgtta gtcggtggag gaagtcgcaa
    10981 cacattgtgg aatcagatta aagccaatat gcttgatatt ccggtaaaag ttctcgacga
    11041 tgccgaaacg accgtcgcag gagctgcgct gttcggttgg tatggcgtag gggaatttaa
    11101 cagcccggaa gaagcccgtg cacagattca ttatcagtac cgttatttct acccgcaaac
    11161 tgaacctgaa tttatagagg aagtgtgaaa tgctgaaaac aatttcgccg ttaatttctc
    11221 ccgaactatt gaaagtgctg gcagagatgg gacatggaga tgaaattatt ttttccgatg
    11281 ctcactttcc cgcccattcg atggaaccgc aggtgatccg cgctgatggc ctgttggtga
    11341 gcgacttgct ccaggcgatt atcccgttat ttgagctgga cagttatgcc ccgctgctgg
    11401 tgatgatggc ggcggtagag ggtgacactc tcgatcctga agtagaacga cgttaccgta
    11461 atgcgctttc actacaagcc ccgtgtcctg acatcatccg catcaatcgt tttgcgtttt
    11521 atgaacgggc gcaaaaagcc tttgcgatcg ttatcacagg cgaacgagcg aagtacggga
    11581 atattctttt aaaaaaaggg gtaacaccgt aatctcatac cggtacgccc gcatgacgcg
    11641 ggcggttatc gaatgatggg gtgaaaaata tgaaagcggc acgccagcaa gcgatagtcg
    11701 acctgctgct gaaccatacc agcctgacca cggaagctct ctctgaacag ctaaaggtca
    11761 gtaaagaaac cattcgtcgc gatctcaatg aattacagac gcagggtaaa attctgcgca
    11821 atcatggacg cgctaaatat atccaccgtc aaaatcaaga cagtggcgat ccctttcaca
    11881 tcaggctgaa aagccattat gcgcataaag cagatatcgc gcgcgaggcg ctcgcgtgga
    11941 ttgaagaagg gatggtgata gccttagacg ccagttcaac ttgctggtat ctggcacgcc
    12001 agttgcctga catcaacatt caggtcttca ccaatagcca tccgatttgc catgaactcg
    12061 gtaaacgcga acgcattcaa ctgatcagtt ccggcggcac acttgagcgc aaatatggct
    12121 gttacgtcaa tccctcgctg atttcccaac ttaaatcgct ggaaatcgat ctgtttattt
    12181 tttcttgtga agggatcgat agcagcggcg cactgtggga ctccaatgcg atcaacgctg
    12241 attacaaatc gatgctatta aaacgtgccg cgcaatcgtt gttattgatt gataaaagta
    12301 aatttaatcg ttcaggggaa gcccgcatcg ggcatctgga tgaggtaacg cacattattt
    12361 ctgatgagcg ccaggttgca acttctttgg taacagcctg acggaaaggg tagcaggccg
    12421 gagacgacgc cccggccttg cctgttatcg ctcgtcgcga cgaccaccca ccgcagccca
    12481 gatgcggcgg acgtgcaccg tcacttcttc gcgatcgtga tacaactgcc gcgcctgagt
    12541 ctgagcattt atgccatgtt catcaagctg tgcctgaata tacgccagat tgtgtgatac
    12601 ttcttcgtag cgttttttca tcggcagttt gagattgaaa atggtttcac ggcaccagcc
    12661 attaaccagc cactgcgcca tcaatgccgc aactttagcc ggtttttcaa ccatatcgca
    12721 taccatccag gagatattgc tgcgtgtcgg acggaatttg aaaccgtctt cccgcagcca
    12781 cgtcacctgc ccggtatcca tcagactttg cgccatcggg ccgttgtcga cggaataaac
    12841 ccacatgttg cgcttcacca gttggtaggt ccagccgcca gggcaagcgc ctaaatccac
    12901 cgcccacatc ccgttcgcca ggcgttcatc ccactcatcc gcaggaataa acacatgaaa
    12961 tgcctcttcc agtttgagcg tggaacgact cggcgcctct gccggaaatt tcaggcgcgg
    13021 aatgcccata tagaacggcg aattattgtt gctgtatgag taaccggtat agcagcatcc
    13081 tggtgcaata aagaatacat gcacaaccgg acgcttcggc gtttcatagt tcgccagcac
    13141 cccagcatcg cgcaaggcag cgcgtagcgg aacagtgaac ttacggcaga atttcagcaa
    13201 ctctttgctt tcgttggtat cggcaacttc aacacgcagt tcaccgccct tctctactac
    13261 accctgtaac atgccgacaa tgggggtaat acgatcttct ggcggcaaat gctgcaggag
    13321 ttcccccacc acaaaccact ggcgggcaaa aattaatgaa ctgaacggca gctcacggat
    13381 taacttatcg ccatcatcag gttgataaca ttcataaatg acataacccg cattctcttt
    13441 cacgcgggca aaaccgaaaa tttcccgctg gccggcttta tcggtaattt ctgcggcgca
    13501 ctctttttca aagcccggac ggcacagcaa tacaacctta ttcatgactt acgcccttgc
    13561 gctttaaacg gatagcacca actaacatta acgcccagcc cgcaaggaag ctcacgccgc
    13621 caaccggtgt gacaaacgcc cacaaacgca gatgggacag cgccaggcaa taaaggctgc
    13681 cgctgaacag caccgtgcct aacgcgagga aaacgctact ccagtaaaac cagatactga
    13741 tgcgacgctg cattgccacc gccagaccta agatcgccag cgtatgaaac gcctggtatt
    13801 cgagaccggt ttggatccag cccatctcaa cggcccccat ggttttactt aacacatgcg
    13861 caccaaaagc gcccagtgcc acaaaaatga agccgctaat agcggcgaaa atcagcataa
    13921 aacggctggt catggtcgta ccctacgtaa attattgttc ataacgaaag cggaattttt
    13981 cttgttcagc agcggctttc gccaggatcc attggcgaaa ggcggctatt ttacccagtt
    14041 ctgcctggct gtcatgacaa accagataaa aagcattttt actgaccaga acatcattaa
    14101 acgggcaaac aagacgtccg gcctcgattt cagattgcgc catcacgtta tttgccagcg
    14161 ccactccctg cccgtgaata gccgcttgca gcaccatggc gctatgacta aaaattggcc
    14221 cttgctgaac gttgatatga ttaaacccca actgtcgggt atatgtctgc cagtcacggc
    14281 gtgacgcatc atgtaataac gtatgtttag ccagatcttc cggggtcttc aagggttttt
    14341 cgcctgtcag cagtagcggc gaacacaccg gcaataaata ttcggcgtac agtttttcca
    14401 cccgtagccc cggccagttg ccccgaccat aaaatatcgc cacatcaaca tcatccgcca
    14461 gcttatcttc ctgacgatca accgcctgga ttcgaacgtc aattcccgga taagctgaat
    14521 taaagctgga aagtcgcgga accaaccaat gaatggcgaa actggggagt aaactgaccg
    14581 tcaacgcccc cttggcgcta cgggcctgga gtttacgcgt cgcttcggtt aattgcgaaa
    14641 atatctcttt gatatcgagg aaatagcttt gcccttcctc ggtcagcagg agtgaacgat
    14701 tacggcggcg gaacagtttt agccccaaaa aatcctcaag agacttgatt tgatgactta
    14761 ctgcggcctg ggtcacaaaa agctcttctg ctgcgcgagt aaaacttaaa tggcgtgctg
    14821 cggcatcaaa aactcgtaag gcatttagcg gtggtaatcg tttagacatg gcttttaaac
    14881 tttgatgtta aatgaattta acaattagat cacactgtgt aacctattag tttttttaat
    14941 ctgagccatt ataaattgtc cgttgagctt ctaccagcaa atacctatag tggcggcact
    15001 tcctgagccg gaacgaaaag ttttatcgga atgcgtattc tggtgaactt ttggcttacg
    15061 gttgtgatgt tgtgttgttg tgtttgcaat tggtctgcga ttcagaccat ggtagcaaag
    15121 ctaccttttt tcacttcctg tacatttacc ctgtctgtcc atagtgatta atgtagcacc
    15181 gcctaattgc ggtgcttttt tttaccttgc gatcgcgaat tactgttcca gttcgaccat
    15241 ctctttcacg tcagtacggt tgatctgctg tttgttgcca ttagcgtctt tatacgaaat
    15301 cataccgata tcgttatcag tctgtggttt gccgtcagag acgatggtac gtccgtcatt
    15361 ggtgtgcatc acatagttcg aaccggaaca ggcgctcagg gcaaaagtca gcatacaggc
    15421 agaaataatt gcggcagtct ttttcatcat cttctcctta aaacgattaa taaatcttaa
    15481 tagtattgat taacaggcta aaattaacgc ctaacactat tcagcatatg ttacttacgc
    15541 ggcttcgcca ggatatccag ataattctga tggttagcac tctccttgta tcaaagtgaa
    15601 ttttgcgtca cgatcggtgc atcaagccga ggagtaccat gaacgttttt aatcccgcgc
    15661 agtttcgcgc ccagtttccc gcactacagg atgcgggcgt ctatctcgac agcgccgcga
    15721 ccgcgcttaa acctgaagcc gtggttgaag ccactcgaca gttttacagc ctgagcgccg
    15781 gaaacgtcca tcgcagccag tttgccgaag cccaacgcct gaccgcgcgt tatgaagctg
    15841 cacgagagaa agtggcgcaa ttactgaatg caccggatga taaaactatc gtctggacgc
    15901 gcggcaccac tgaatccatc aacatggtgg cacaatgcta tgcgcgtccg cgtctgcaac
    15961 cgggcgatga gattattgtc agcgtggcag aacaccacgc taacctcgtc ccctggctga
    16021 tggtcgccca acaaactaga gccaaagtgg tgaaattgcc gcttaatgcg cagcgactgc
    16081 cggatgtcga tttgttgcca gagctgatta ctccccgtag tcggattctg gcgttgggtc
    16141 agatgtcgaa cgtgactggc ggttgcccgg atctggcgcg agcgattacc tttgctcatt
    16201 cagccgggat ggtggtgatg gttgatggtg ctcagggggc ggtgcatttc cccgcggatg
    16261 ttcagcaact ggatatcgat ttctatgctt tctctggtca caaactgtat ggcccgacgg
    16321 gtatcggcgt gctgtatggc aaatcagaac tgctggaagc gatgtcgccc tggctgggtg
    16381 gcggcaaaat gattcacgaa gtgagttttg acggtttcac cactcaatct gcgccgtgga
    16441 aactggaagc aggaacgcca aatgtcgctg gcgtcatagg attaagcgcg gcgctggaat
    16501 ggctggcaga ttacgatatc aaccaggccg aaagctggag ccgtagctta gcaacgcttg
    16561 cagaagaagc gctggcgaaa cgtccaggct ttcgttcatt ccgctgccag gattccagcc
    16621 tgctggcctt tgattttgct ggcgttcatc acagcgatat ggtgacgcta ctggcggagt
    16681 acggtattgc cttgcgggcc gggcaacatt gcgctcagcc gctactggca gaattaggcg
    16741 taaccggcac actgcgcgcc tcatttgcgc catataatac aaagagtgat gtggatgcgc
    16801 tggtgaatgc cgttgaccgc gcgctggaat tattggtgga ttaatgacaa acccgcaatt
    16861 cgccggacat ccgttcggca caaccgtaac cgcagaaacg ttacgcaata ccttcgcacc
    16921 gttgtcgcaa tgggaagata aatatcgcca gttgatcatg ctggggaaac agcttccggc
    16981 tttgccagac gagttaaaag cgcaggctaa agagattgcc ggatgcgaaa accgcgtctg
    17041 gctgggatat accgtggctg aaaacggcaa aatgcacttc tttggcaaca gcgaagggcg
    17101 cattgtgcgc ggcctgttgg cggtgttgtt gactgccgtt gaggggaaaa ccgccgccga
    17161 attacaggca cagtcgccgc tggcattgtt tgatgagctg ggattacgtg cgcagcttag
    17221 cgcctcacgc agccaggggt taaatgcgtt aagcgaggcg attatcgctg cgacgaagca
    17281 ggtttaagca cagaaccggg tcggataaga cgttcgcgtc tcatccgacc tgattgttta
    17341 accctgacgc gccgctttcg ccatcatctt cttcagcgca tgagaaaccg caacaaaccc
    17401 aaaggtggcg gtcaccatcg ttgccgcgcc aaatccagat gcacaatcca tccgcttcgg
    17461 cccttctgcc gtggctttca tcgcacacac cgtaccgtct gactgcgggt acaccagcgc
    17521 ttcagtagaa aacacgcaat ccacgccgag cttaccttta ctgtttttca ctacgccaaa
    17581 atcgcttttc aggcgctcgc gcaacttcgc cgccagcgga tcctgaatcg ttttcgccag
    17641 atcggtaacc tgaatctgcg tcgggtcaat ctgcccaccc gcgccaccgg tcgtgaccag
    17701 cgggatttta ttgcgccgac aataagcaat cagcgccgct ttgggccgta cactatcaat
    17761 ggcatcaatc acgtacgaat aaccggcact catatattgc gctacgttat ccggcgtcac
    17821 gaaatcatcc accaccgtta cacggcactc cgggttaatc tggcgaatgc gctccgccat
    17881 gacttccgct tttgccagcc cgacgttatc gcgcagagcg tgaatttgcc gattggtatt
    17941 ggtgacgcac acatcatcca tatcgataag cgtgattgcg ccaatccccg tgcgcgccag
    18001 cgcttccgcc gcccaggaac cgacaccacc gataccaacc acacaaatat gcgcgtcagc
    18061 aaacagttgc aacgcttttt caccatacag acgcgctgtg ccaccaaaac gctgacgcca
    18121 tgcatcacta attaccacag acatagcacc tcagaattaa aaagggcgag gttatccctc
    18181 accctaaacg cataaatgcc tgatgcgcta cgcttatcag gcctacataa ctcctgcaat
    18241 attttggatt tgcatgattt gtaggccgga taaggcgttc acgccgcatc cggtatgaac
    18301 aacgcgcact tagtcaataa tcaggatgtg aggttacccc tcaccctgtc atatccgtaa
    18361 aaacggcata cagaatatca catcagccgc taaagacgtt acctgcgccc ggagccgttt
    18421 tcagcaccca aacccgtcca tagtggttgt accaacctgc gcggtgtccg gcttccggcc
    18481 cgatcccttg atagatatcg aagtgttggc ctttgattgc gcccccgaca tccaacgcca
    18541 ccatcagacg caattcgtac tgaccgttaa atttgccgtt attatccagc agcggcactt
    18601 ctgccagcaa ggtagtacct ggcggaataa ttgaacgatc ggaggcaaca gaagcacggc
    18661 caaccagcgg caccgcactt gcccctttca ccggtgcaaa agattgcggt ttaaagaaga
    18721 cgaaagacgg gttctgttcc agcagctcgc gaacctcggc ttcactgtgt gtttcgcccc
    18781 aatgacgaat cgcctgcatc gacatatctt cttttttcac ttcgccacgg tcgatcagca
    18841 ccttaccaat gctgcgatag gcatgaccgt ttttccctgc atagctgaaa aagttaagcg
    18901 gactgccatc accaaagtcg atatacccac taccctgcac atccataatg aagttatcca
    18961 tcagagagtt gctgtaagcg aggatgtatt tatcgcccaa tgccccggcg tagatctccg
    19021 cacgagacgg cagacgacca cgttttggcg gcatacggta aataggatac tggaactcgc
    19081 cctggcgggt atggcgcgcc tgaattaccg gcgtgtaata gcctgtaaac tgcacgttac
    19141 catagttgtc ggcaccttcc atctgccagg catcaatgcc gaactggcgc atattgcggg
    19201 tatcaccgcc tgcgcgcagc cactcttgca ccgcgttata aacattactc tggttgccat
    19261 acagacgcgg tgacgaatta cggatatggt taatttgctc ggcaaaatca ccggcgttaa
    19321 tcggcgcgcc aacggcatct ggctggttca ccagagagaa aggctgggta aatttcccgt
    19381 ctttatattg ctgtccgcga tcggttggtt tggaagagca ggcggcaagc attgccacaa
    19441 ccgtgcccat aagaaggtac tttacccaac gtcctttcat tgttctcttc ttcaggttaa
    19501 aaaataaggc gcaacgaaga taacaaaccg ccgcgtttaa tgaaatgagc agcctctccc
    19561 tgacgcaaat tttgcacaaa aaataggctt tagtggtttg tttttgttca aaatcatgct
    19621 aaatccgtga tcggggtaaa aaaaaggttg catgaaaacg cgagcggagt atagtgcgca
    19681 tccacggacg cggggtggag cagcctggta gctcgtcggg ctcataaccc gaaggtcgtc
    19741 ggttcaaatc cggcccccgc aaccaatcaa atttgatgaa gtaaaagcag tacggtgacg
    19801 cggggtggag cagcttggta gctcgtcggg ctcataaccc gaaggtcgtc ggttcaaatc
    19861 cggcccccgc aaccaattat tgaacaccct aacgggtgtt tttttgtttc tgatctccca
    19921 taaaaaaagc gccattcagc gcctttttat catccccttc tcgccagcgt tgccccatcg
    19981 gcaaaatacg ctttaatccc cgcaagaata gactccgcaa cttcctgctg gaaagtcgcc
    20041 gttttcagtt tacgctcttc ctcaacgtta ctgataaacg ccgtttcgac cagaatggag
    20101 ggaatatctg gtgcctttag tacggcaaac ccggcctgtt caacttgatt tttatgcagc
    20161 ttgttgattt ttccgagctt attcagtacc gctttaccaa acttaaggct gtcagcaata
    20221 gtcagcgact gcaccatatc gaacatggtg tggtcgacat agcggtcacc gcttttgctc
    20281 acgccaccaa tcaagtccga ggcgttctgg gtttgtgcca gatattttgc cgcagtactg
    20341 gttgcgcctt tggttgagag cgcaaacacc gaggaaccgc tcggctgacg actggtaaag
    20401 gcgtcggcat ggatagagac aaacaggtca gcacgctgtt tctgggcttt tgctacgcgc
    20461 acttgcaacg gaatgaagat gtcttcattg cgcgtcatgt acaccttcat attgccctct
    20521 tcctcgatca gagagcgcaa acggcgagct atttgcaata ccacatcttt ttcgcgcgtt
    20581 ttgtatttcc ccaccgcacc ggagtcttcg ccaccgtggc cagggtcaag cataatgaca
    20641 atcggacgat cgcgccctgc tttacccggt tgtggaccac tttgcgctgg cggcacctgc
    20701 ttttcgaggt cgcctttgtt gtaatcctcc agcagcgcca gcagcgggtc ctgcatatcc
    20761 tgtgcattgg ccggatagag gtccatcacc agacgctctt taaacccggc gaccggcgca
    20821 agggcaaaca gctgcggttt tacgttttgc tttaattcaa aaaccatgcg tacggtttgc
    20881 gggtcaaatt gcccgacgcg cgccgacttg atgaacgggt cgtctgcacg aatttgtgcc
    20941 gccatcccct tgagtaccga gttcaggttt acatcttcga tatccaccac cacgcgttca
    21001 gggttactca acgcgaactg cttatatttc agctgacgat ttgattctac cgtcacgcgg
    21061 gtgtagctgg acgcaggcca gacgcgcacc gccacgacct ggctaaccgc agccaggctg
    21121 acctgactta cgctcaatag ccacatagcg cccgcgcctt gcagtaaacg acgacggctg
    21181 attgcagtgt tggatcctga catgcctctc ccgagcaaaa gaaatctaag ctgtgtaaca
    21241 agtaaacgac taatttgacc ggaaacttta gcgaaagacg cataatctgt catctaataa
    21301 acggtaaaca ttctttttat attcacggca ttactgataa aaaagtcgct ctcgcataaa
    21361 atttacactt gcaccctgcg aaaaaacaga ataaaaatac aataatttca aataatcatg
    21421 caaaggggtg tgccgtggta aaggaacgta aaaccgagct ggtcgaggga ttccgccatt
    21481 cggttcccta tatcaatacc caccggggaa aaacgtttgt catcatgctc ggcggtgaag
    21541 ccattgagca tgagaatttc tccagtatcg ttaatgatat cgggttgttg cacagcctcg
    21601 gcatccgtct ggtggtggtc tatggcgcac gtccgcagat cgacgcaaat ctggctgcgc
    21661 atcaccacga accgctgtat cacaagaata tacgtgtgac cgacgccaaa acactggaac
    21721 tggtgaagca ggctgcggga acattgcaac tggatattac tgctcgcctg tcaatgagtc
    21781 tcaataacac gccgctgcag ggcgcgcata tcaacgtcgt tagtggcaat tttattattg
    21841 cccagccgct gggcgtcgat gacggcgtgg attactgcca tagcgggcgt atccggcgga
    21901 ttgatgaaga cgcgctccat cgtcaactgg aaagcggtgc aatagtgcta atggggccgg
    21961 tcgctgtttc agtcactggc gagagcttta atctgacctc ggaagagatt gccactcaac
    22021 tagccatcaa actgaaagct gaaaagatga ttggtttttg ctcttcccag ggcgttacta
    22081 atgacgacgg tgatattgtc tccgaacttt tccctaacga agcgcaagca cgggtagaag
    22141 cccaggaaga gaaaggcgat tacaactccg gtacggtgcg ctttttgcgt ggcgcagtga
    22201 aagcctgccg cagcggcgtg cgccgctgtc atttaatcag ttatcaggaa gatggcgcgc
    22261 tgttgcaaga gttgttctca cgcgacggta tcggtacgca gattgtgatg gaaagcgccg
    22321 agcagattcg tcgcgcaaca atcaacgata ttggcggtat tctggagttg attcgcccgc
    22381 tggagcagca aggtattctg gtgcgccgtt ctcgcgagca gctggagatg gaaatcgaca
    22441 aattcaccat tattcagcgc gataacacga ctattgcctg cgccgcgctc tatccgttcc
    22501 cggaagagaa gattggggaa atggcctgtg tggcagttca cccggattac cgcagttcat
    22561 cacggggcga ggttctgctg gaacgcattg ccgctcaggc gaagcagagc ggcttaagca
    22621 aattgtttgt gctgaccacg cgcagtcttc actggttcca ggaacgtgga tttaccccgg
    22681 tggatattga tttactgccc gagagcaaaa agcagttgta caactaccag cgtaaatcca
    22741 aagtgttgat ggcggattta gggtaagcga tgaaaatcac cggatgcggc aggcgtaaca
    22801 ctcgtgcgtc gcatccggca attacgttta ttcccgcgaa ctaaacaacg ccgccagacc
    22861 actgcgccgc tcagtacgag tggcgattgc cgcacttaat atacgctcat cggcatacag
    22921 cgacagacga cgacgcgcgc gggtcaccgc ggtgtatacc agctctcgcg ttactaccgg
    22981 cgtgcgttgg ctcggtaaaa tcaacgccgc atggtcgaac tccgatccct gcgatttatg
    23041 taccgtcatc gcccacgtag tttcgtgctc tggcaagcga ctcggttgca cagacttaat
    23101 attgccgtcc ggcatcgcaa accagacgcg cgtcccctgc ccgcgatcca gcgcaatacc
    23161 gatatcgcca ttaaacaacc caagcgcgct gtcattacgg gcaatcatca ccggtcggcc
    23221 ttcgtaccaa cgagagtgcg gatgacgatg aattttgcgc ttctgttgca taaactgttc
    23281 aattcgctca ttcagtccag ccacgccaaa cggcccttcc cgcagggcgc acaaaagctg
    23341 gtactcattg aacgcctgaa tgattaaatc cggctcggca cgcgcttgca gcagatccag
    23401 ataacgtccg taacccgcaa gagcttcctc aagcatcgca atataatctt cgccgctctg
    23461 taaaagtcgt ttttcgatat cagtaaaatc ctgctgaaaa acggttttca ctgccgtttt
    23521 atcaccacgg ttgatcgccg cagctaactg accaatgcca gaatcgctgc cgaaacgata
    23581 gcttttttgc agcaggcaga gactgtcgcg caaagatgcc gcttctgtgc cggttcctgc
    23641 cggaacgtga gtccccgtca ggcggcttag ctgcctggca cgctcggcgg taaagcccgc
    23701 gttggcataa gcgcagatat cgcccagcac agccccagcc tcaaccgagg ccagttgatc
    23761 acgatcgccg agaaagatca ctcgcgcatg atctggcaag gcgtcgatca gtctcgacat
    23821 cataggcaga tcgatcattg acgcttcatc taccaccagc acatcaagat gcagcgggtt
    23881 accggcatga tgacgtaaac gctggctacc cggctgcgcg cccagcaatc ggtgcaaagt
    23941 gctggcatct tccggaatgc gtttcttttg ttcatcggtc agcggtaact gtcgcaaagc
    24001 cttgccgaga gattcggtta agcgcgcggc agctttaccc gttggtgcag ccagacggat
    24061 acggcagcgt tcgccgtcgg ccatttgaat taacgctgcc agcaacttcg ccacggtggt
    24121 cgttttaccg gtgcctgggc cgccggaaat caccgagatc cgccgcgtca gcgccactgc
    24181 cgctgcaact ttttgccagt taatttcatc gcttactgga aaaagtttgt ccagggtttg
    24241 cgccagtaga gcttcatcaa cctcaatggt atgattcacc tcattgaaga agcgcgctac
    24301 cgtccgctcg ttacaccaca tgcgattcaa ataaagacga tcgccacaga ggatcatcgg
    24361 cgtgggttca tctcccctgc tgaccgcttg agaagccagc aagcattctt cccaattttg
    24421 tagctcaccg atttcactga cacaggtcgc caacagcgga ttcgacgcct cgttattttc
    24481 cagtcgtgaa agcggcaaac agacgtgtcc ctctccggca tcatgactta acagtgccgc
    24541 cgcgagggtg acggcaggat gttcatctcc cgccacggtc agggcaaatt gcacatccag
    24601 cgggcgtagc tgtttgtgct ccacagcttc cagtaattgc ttttgcaatt tcattacgcc
    24661 tcctccaggg tcataccggc aaacatctca tccatcaggg caatcaaccc ggcgttgggt
    24721 cgggttgtgt aaatcccttg ttgcggatgt tctttatcaa cgccacgcag gaacagatag
    24781 ataacgccgc caaagtgaag atcatagtcg taatcagcaa tgcgatggcg cagataacga
    24841 tgcagcgcca gggtataaag ctgatattgc agatcatagc ggtgtgcctg catcgctgct
    24901 gccatagcct gttgggtgta agccgaactg tcttcaccca gccagttgga tttatagtcg
    24961 agcaggtaat aacgcccttc gtggcggaat accaggtcga taaagccttt taacatgccg
    25021 cgtacctgca tgaactccag cggcgggcag ccagcggata gcgggtcaaa ctggcgaatt
    25081 agtgcatcaa gctggctggc gatgagcggt tcgctaatcg gcaggtaaaa ctccatctcc
    25141 acctgtttat cgcggtcgga aagctgactc aggctaacac ccgtttcatt gagaggtgcc
    25201 tggaggacag ccgtgatcca ctcggtcaac accggttccc actgcgattc aaagccgccg
    25261 agttccagtt tttcctgtac ccagttcggg tcaaccggct gggtaaaatc gaggtcttca
    25321 aacaaactgt gcaagaatgt ccccggtgac gcaccgcgcg ggaactgatg cggtgttaac
    25381 gtcggttctt caacgacgct gaccacgcct gcggcatcaa catccagccg tggcatcaga
    25441 tcctgagcga taccgtgacc acgctgctgc aagccggagt agctggtgac acgccagtta
    25501 tcgccgggca atcgttgtag tgtcctcgca ttcagttctg cagttaaagc atcattaacc
    25561 tgccagggct ggttatcacc agtttgtgcc gtttgccagg caatatcatc atcgcataac
    25621 gcttcaatac aggtgcgaag ccctgccgca tcttgcggtt cccctttttg cagcaaacgc
    25681 ccgagcgcac tttggtggac gtcggtgtca ccttttttat cgccacgacg gcgcaccagc
    25741 ggtgcaacgc cgagactgca atgccaaacc gaacgcgtca gcgccacgta aagcaaacgc
    25801 agatcttccg ccagacgttc gacctccgcg aggtcgacgc tttctggcgc agcattaaga
    25861 tccaaaactg cctcaaacga gtggcgatcg tgataaaacg cctgatcctg gacgcggaaa
    25921 ttggtaataa atggcaacca gaccagcgga tattccagcc ctttcgattt atggatcgtg
    25981 acaatctgca ccagatgttt atcactttcg aggcgcattt gttggctgga ggcattactg
    26041 tctggctcga ggatatgttg cgataaccag cgtaccagcg catgttcact ttccagctgc
    26101 gttccggctt cttgaagcag ttcgctgata tgcaagatat cggtaagacg ccgctcaccg
    26161 cctgccgttg ccagcaagtt ttctgcaatg ttacgcgccg acatcagcgc ccgcagcatc
    26221 ggcataacgc cacgtttgcg ccagatttgc cgataaccat cgaactcttc gactaccgca
    26281 tcccacgcat gttcgtcatt attcagcgtt tcaatatcca gcgcattcag ccccatcatt
    26341 gacgttgcca gcgcactgcg cagggtgttc tcacgttcgg gcgtcatcac cgcctgcaac
    26401 aaccaaagca tttcctgcgc ttccagagtt tcaaaaacac tgtcgcggtt cgaaaggtaa
    26461 acggaaggga tttccagcaa cgttaaggca tcgcgcacct gggcggcctc ctggcggctg
    26521 cgcaccagca cgctgatgtc cgaagcacgc accggacgcg cgtcgtcgcc gttcatcagc
    26581 aacgcttcgc cccgctgtcc ggcttgtagc cagtcgcgga tttgcgcagc acatacctgc
    26641 gccatggtac tttgatagtc gccaacgccg cagctttcgc cttccatcag ccacattttc
    26701 atcgcaggct gcgtttcacc tttaaataca aaacgtaacg cctgattttt cccggctgat
    26761 ttcactggaa taaacggtat ttcgcgaaac atgaacgtgt catcagtctg gctgaaaagc
    26821 ttgttcacac tgttcaccat tccaggtgcg gagcgccagt tggtgtctaa agtgtagtgg
    26881 gcgtgaactt cactacgcgc cttcatataa gtgaagatat ccgcaccccg gaatgcatat
    26941 atggcctgct tcgggtcgcc aatcagcaac aatgcggttt ccggctgatg gtgccagata
    27001 cggcgaaaaa ttcggtactg ctgggggtcg gtatcctgaa attcatcgat cattgccacc
    27061 gggaatcgcg tacggatcgc cgctgccaac acctcgccgc tttcgctacg cagcgcggaa
    27121 tcgagccgac ttaacatgtc atcaaaaccc aattcgccac ggcggcgttt ttcacgcgct
    27181 actgtttcgc ggatctcagc caatgcgcgg gtgatcaaca gatcgcggat cgacaatggt
    27241 tctgcaagca gttgatcgat cgcctcaaac agtggatgtc gcggggtttc ccccccggcc
    27301 ttcgtgcgat cttctaagaa acgctgggag aatttttcca gcgactccgg caactgataa
    27361 ctgtttgtct cttcttctgc ccaggcgctg atcttttcga tccatttagc ctgattgcta
    27421 cggttaaact tgcgtcgatc aataccagaa gattcgatca gcgcatccag ttcacccact
    27481 gcgtcgcgcc actgctgttt taccgtatca atacgcgcca caatttgcgc gtggcgggaa
    27541 gccagcgttt catcatcggg cggcggtgct ttgataaccg gcggttcgcc ttgcagataa
    27601 cgattaatat cgcgcagcaa cgcctgcggc cctttccagg tttcaaagac gacctgggct
    27661 atttcacgcg gcagcgggta gcagtggcga cgccagaaat cggcgcaggc ctggtagcgt
    27721 agcagagact catcttcaat cagctgctgc tcaaacagca tgccggattc aaaggcattc
    27781 aggttgagca tgcgctggca aaagccgtga atagtaaaga ctgccgcttc atccatctgc
    27841 cgttcggcta acaacaacca ctgcgcggct tgcgctttat cgtcgatctc ttccagcagg
    27901 cgttcgtaca gtggattgtc ggtggtttca cgcagacagg cgatgcgcaa ctcgtggata
    27961 ttgctacgga tacgaccgcg caattctgcc gtggcagcct cggtaaaggt gaccaccagc
    28021 agttcttcaa cgatcagcgg gcggggaaag gcggcggaac cgcctagtcc aagtaacagg
    28081 cgcaaataga gcgccgcaat cgtaaaggtt ttgcctgtgc cggcagaggc ttcaatcagg
    28141 cgctcgccct gtaagggcaa gcgcaaagga tctagtgtct cggcgacatc actcattctt
    28201 ttcactcatc aggggcattg tttgctgcaa cgcgctgacg ttctcccaca ctttccagcc
    28261 ttcaggatgc acatattcgg ctttcccgtt ctggctgccg gaaatctgcg acagaatagc
    28321 catgccttgc ggctcgacca ccgcctgatg gaagaaatca gcaagttttt gcggcgtcag
    28381 cagttttatc tgggccacga ttttatcacg cgaatcgaag cgcatattgc cgcgatcgaa
    28441 atctttactt aacttcgatg cttcttcgcc gagcgtttgc ggtgcctgca gcatctgggt
    28501 aattaccgcc tgctggattt gcgcaaactc atctggcttc atcgctcgca attttgcctc
    28561 tgcggttggg aaaaacgcct tgtaacgctc ccacaagaat gaaggctgtt tatcattgct
    28621 ttgcaaaagg aagcccatgc cccactgacg ccccacgctc attggaaacg caaacacggc
    28681 atagcccaat tgttcttcgg tacgcaactg attgtagaac cacggctgta cgatctgccc
    28741 caacagagag ctataggctg agctggtgta ttcatcgtag ccagtcggta caaataccgc
    28801 tgccagtgcg gagtcggtgc tgttaccggc tttttcaaag atgacggatt gttttttatc
    28861 gaccactaca tctttgtttc gacaccactc tgaaccatca gcgcccaact gtttttgcac
    28921 atcgcgtgcc agcgttgttg cctgggcctc ggtcatgttg ccgataacca taaactctgg
    28981 tcgagcccct gattttaagg cgtcgcgata ggccagcacc tctttcaacg taatggaggg
    29041 caaaatttta cgccgttcat ctcgcgagaa gtacggcact tgcgagagca tctgcgcggg
    29101 cataatcgcc tgctcaaacg ctttaccctt ttctgcggaa tccatcatct ggttatacca
    29161 ggacttcgcc tgctcaagct gctcttccgt agcggtatag ctaaagtacc cctcgagcaa
    29221 tgcctggaac agctgcggca gacgctgggt gtaaccatta gcattaatca taaggccgtt
    29281 gttagcgttg gtggaaaaac ttatgccacc aaccgacgcc tggttgctta actgatcaag
    29341 cgccagccct gcgagataat cattgagcgc aaacatcacc tgattgcggg cgctgtccat
    29401 ggctttcgga ttacgcaaaa tcaggctgac atcagctttg ggctcgctgg caaaataacg
    29461 gcttggcgca tacaccacgc gcagattcga ctcatcaaca atcagctctg gatggtcgta
    29521 tttcttctct gacttaatca gcgagaaatc atcaggaata taagggttaa gctctggcaa
    29581 agagagcgca atgtcggcgg cttttttctg ccagtcggcg aaagtttgtg cgctgatttt
    29641 atcgacctga tacggcgcat cgacaaagta agccgttttg ttgtgcggct ctttcgggct
    29701 gatataccag atacgcgcat tctgcggcgt catcatcgcc agacgttcct ttactgcttt
    29761 agcatcgtac cgatcggcaa tattgactgc atccagcgta tgctcaacag gaacgcgaat
    29821 catggtatct gccagccatt cgacgtaatc catatcacgg gtgatcgacg gataacggaa
    29881 gtcgatatcc agcacattcg ccagttcatc gaagtattgt ttatcaatgc ctttttcacg
    29941 taacagattg agatagctaa aaattgccgc cacaacctga tcgcgattag ccaagccttt
    30001 atcagttaaa gacgcagaga tcgctaatac gccgctgttg ccgttgacga taggatcgga
    30061 gttggcgcta atgccctcaa ctaatccctg cttttgcagc cagtcagaaa gtgtacctgg
    30121 gctgcgattg ccaatcagat aggtaatcaa ttcatcggtt ttactacgga acttcgctga
    30181 gttgttatcg atgcgaaact caacgcgcaa cactttacgc ggcagcgcag ggacgtaatg
    30241 aatgataatg cccttttgcg cgtcggtgac taccggcacg gtgatttccg gttttttgct
    30301 ctctttgttc ggcacgcgac caaaggtgtc cgccgccatt tttgccaact ccggcagcgg
    30361 tttattactg taaataaccg ccttcatcaa attggcggaa tagtacttct cgtggaaatc
    30421 tttcagcgcc tgctgcaccg gattaccagg tttgtcgctt aaagtttcga ggttaccacc
    30481 agaaaacttt gaaccggggt gtgccgggtt aatggtttct gcgctgacct gtgccatgcg
    30541 catcccgtca cgcgtacgcg ccatggttaa ttcagcgttc actgcattac gctcacgttc
    30601 ggcgtatttc ttgtcgagca agggttctgc aatagcatcg gccaggcggt ctaccgcacc
    30661 gggcaaggcg tcgttctcaa cttccagata gaaagccgtg cgatacggcg cagtgctggc
    30721 attgtgacta ccgccgtgca ttttgagata ttcggccaga ctgtcagcct gcgggtactt
    30781 tttcgacccc atcagactca tatgttcaag gtaatgtgcc agcccctggt acgcctcggg
    30841 atcttccagc gacccaacgg gcactaccag cgccgagagc gatttaactg cctgcggatc
    30901 agaaaccagc aagaccacca taccgttatc cagacgtata gcctgatact ggcggttatc
    30961 tttatcactt ttacggatgg tttcctgaat cggctgccat cccgtttctg cctgacttaa
    31021 tggtgcccag agggcaacaa acaacaataa tgctttgaac caggtgctgc ggggcattca
    31081 cggacctcat aatcaacttt attttctgga cagattcaac aggttaacgc aaatcatctg
    31141 ccagaattta atcttgtgct gcacgagtca gcctatgttt atataaccat cagtccgtga
    31201 ctggtgcgca tcataaagta agcggataga ttgcgcaatt tttatacaac actcatgact
    31261 gattaaagcg aaacagcggt aacaggaaac gttgcgactg ttcaacgatg gcctccattg
    31321 tctctggtgt taattgccgc cagagccgtt gataccagat atcatcacct tcgccacgca
    31381 ccatcatgtt gccttcgtaa gcctgaagga atttcgtacg ggctttttgc aacgtggaat
    31441 cgtcatccag catggcatcg ttttgcgcgt cataacaggt ttttagccac gcgccgccac
    31501 tttcaggtaa caccagcaat ggcgcggaca ttccttcacg atacccctca atcagttgtg
    31561 agaggtaatg caaagcctgt tcggctgcaa gcggcggaaa acgccactca ccgtctttgc
    31621 gtagaaaaag gcgactttcg ccattaccac cgctggcaca gtagacaagg tgttccagcc
    31681 aaagttgcat tccctgcgcc acacttaata aagagggacg ccagcgcaac aggccatccg
    31741 gctgcacctg cggcaaccag ccagttatct gcacaccgtt gcaggcgaga tcaatttcca
    31801 tactctgccc cggctggcga caggcaatga ctctgtcggc aagctgctgc atctcctggc
    31861 actgtgtttc ccagaaaatt tcaccaaaag cgccatacgg taaatcccct gccgcccgga
    31921 agcggcggaa caagcgttcg gcatcatcct gctcaaccag tgcattcaat aactgctgat
    31981 tgatttgata acggctaagt ccttccagaa taaatggctc ggtgtctggg atttcgctgt
    32041 cttcagtatg aaagttcacc tgcaaacgca tctggaagaa cgcccgcacc ggatgtgccc
    32101 agaatcgttg tagcgtttcc agcggcacgg tttccggtaa ggtaaacggc agcggctgaa
    32161 caaattcaga atgtgcttta ccagcctggc tggccgcagg tagccattca cgagcatagc
    32221 tttgtcgttc gcctggctgg tagttttgcg gatcaaacgg catccgggta tggaggcaag
    32281 taagatgcgc ttttaccctt gcctcgcttt catcacagtt gagcgcttca tcgcccggta
    32341 gataatgact ttgcccgatg tagtcgatca gttcctgcac cagtaccgac gggaaacgtt
    32401 cactgttatc ctgaatggaa cgcccgatat agctgatata gagtttttgc tgcgcggaaa
    32461 ttaacgcttc caggaacagg tagcggtcgt catcgcgacg gctacggtcg ccacgcttcg
    32521 gtttctggct catcaggtca aagcccaatg gcgcaagctg acgtggataa acgccgtcgt
    32581 tcattcccag caggcaaacc actttgaacg gaattgaacg cattggcatc agagtacaaa
    32641 tgttaaccgg tccggcgaga aaacgctggc tgatacgttc ttgatccaga cgctgtgcca
    32701 gttcatcacg caatagtgac agcggcaccg cgtcgccata ctgcgcacct aaaccttcgg
    32761 cgataatcgc ctgccattgt tgttcgatca gcgtcatcgc cgcttcggtt tccgcatccg
    32821 gcaggaagaa ggcgttgagc atatcgcgac aaaccggcaa ccactcttct agcggacgct
    32881 cctgtgccag cccgcgacgc cagatgttta gctgcattag cagtgaagcc agatgcccca
    32941 ccagttctgc aattaagccg ctcgattcat cataaggtag aaccgattgc cactcgccct
    33001 gcgcgctctc catcgcgtag cccagcaaca tacgcgtcag gccaaatcgc caggtgtgtt
    33061 gtccggtggc ggggagttcc agctcgcgaa cgttgtcgtc atctatcccc caacgaatgc
    33121 cggattcgtt gacccactgg cgtaaataac gcagcccttc ttcggtgatg tcaaaccgcg
    33181 ccgccagcac cggcacatcc agcagcgcca gcacatcctc tgacacaaag cgactgtcag
    33241 gcagtgataa caggctgata aacgcttcca gtaccggatg tgactgccgc gcacgacggt
    33301 cggaaatggc gtaaggcagg taacgatccg caggtgcgct accaaacact gcctgaataa
    33361 acggactgta gctgtcgata tcagccacca tcacgatgat gtcgcgcgga gtaagtgtcg
    33421 gggcttcctc cagcatcgcc agcaagcgat cgtgtaaaac ttcaacttca cgctgcgggc
    33481 tatggcaaac gtggaatgtg atactgctat ccagtggatc aagcgggcgt ttgttatcgc
    33541 tacgggaaaa ctcttcgatg ttcacacccg caacggcgcg gttttccagt tccagaatgt
    33601 cagactgaat gttatgcagc aggttatctg gcgtcacatc gacaaaagcg tccagctcct
    33661 ggctgctctc caggtcagaa aggagataaa tgtagtcgcg cccaagcttc ccccatgagg
    33721 ccagcagcga gttgccgaca tcctgttcac catcgctgtt aaagagctgt ccggcatttt
    33781 cgctatcgcg aaatagcggc aattcgcgat cttcaaaact gtgtcgacgc tgacgagtca
    33841 gcagtttcgc cagataagca ggatctttaa tatcgcccca gtaataacgg caggggttgg
    33901 taaacaggag atggatttca atatgtttac ccagcgcctg gagcgcctgg agataaacag
    33961 gcggtaacgc ggaaataccg catataaaga cgcgcgaagg taaccccggc gggcaggtcg
    34021 tcgcggactc cagcgtttcg ataaagcgct gatagagatt cgcgcggtgc cagcgcggtt
    34081 gtccgagttc atgggtatat tccaccagcg ccttccacaa cggggcttgc caggcctgtg
    34141 cttctcccag cccttcaacc aaatgtcctg tttcccactg tgccagccag tccggacgat
    34201 agaccagata ctggtcaaac aggtccgccg cttttgagga aagctggaac agttttcgct
    34261 tgtcgctatc gtcagtcaga taatgccgca acagggtaaa gtcttcgcgc tccagcaatt
    34321 gcggcagcag agtcatcagt ttccagctca tgctctgttt gttaaaggcg ctctctttgg
    34381 ggatttccgg taacacccgg acgaacatat cccagataaa gctcgctggc agcggaaaat
    34441 caatgtttgc cgcaatacca aacttttgcg acagggtcat ttgcagccac tgcgccatac
    34501 cggtactttg caccagaatc atctctggtt cgaaaggatc gtccagccgt tcgcgttcga
    34561 caataaactc catcaacgct tccagcacgt ccagacgatt ggaatggtag acccttaaca
    34621 tagcggctcc tgactactga cgattcgggc aatgcaggcg ggtcatctcg ccttctctgc
    34681 ccccgggtga aactagcgta acgctgatgc tgacacatcc cgcctgcgat gtctgcattc
    34741 ggttgacctg ccagttggca ggtggcgaaa tcgcgcgcag ttgcgtttgc tgccaaccat
    34801 gccgccagag ctgttggtac tggtttctgc tggcgagact gttcattgat gttcgctgga
    34861 tacccgataa cgcagtgaca atcatcacca tcagcaccat cagcaccatc gcaaccatta
    34921 cctccggcag gctaaagcct tgttgattct tcagggaagc tgacataatg ccccctcttt
    34981 cagcggacaa aaatcgctcc agccgcgtgg cgaaaaaaca atttttccat caatgacttc
    35041 gcctgttcgc cacaacgaaa cgccttcata accggcaatc aataaggctt cattatctgc
    35101 cagtaaacgc aaacaaacct gggcatcggt tccagcgtac tgcgagcact gaactgctgg
    35161 ctgcgtctgc caggagtgca tttttcccca cgccagcgcc gactgaacga tggcctggcg
    35221 gcgcaatgac tgactttcca tgctcacgcg agaggcaaaa ctgcgatcct gctgactcat
    35281 tccttgcaat agcaagctac ccaaaaccag cagcatcagg accagagcca gtgacgaaac
    35341 acctttttcg cggttcacag gttgaatcct gtcacgctat agctggcatc caccacggtt
    35401 tgcggttcag acttactggc agcacgcata ttaaccgtca acaccggcga gaagccgctg
    35461 acatcctgac gtacgacctg aaaagtgtcg ataatgatgg catccggatt agtgacttta
    35521 tcccagccct taccttcaca ggatgtcgca ccgcgtagcg tttccagcac atgctccttc
    35581 agacgaaatc caatctggtc ggactctttt accggttcgc gatcccagat accgttactg
    35641 ttcgcatccc actgcacaat gatacagtca ccctgtccga caatttccag cccttcgccg
    35701 gtacagctgc catgacaata acccgccctc tggagatgct tcgcgacggt aaatacccgc
    35761 agccagattt catcttccag tgccagctta cgagtgctcg ttaaactttc acgctgtaac
    35821 gcaggcagaa agcgtgcagc ccccagcaac aatacgctac tgatcgccat agcaatcaac
    35881 acttccagca gagaaaaacc ttgctctttt acaggcatcc ttctgtttct ccttgctgat
    35941 aaagccggag tcttccccac ggcgaaacca ccagccacca ctcgcccgtt gagtttttga
    36001 agcgaatatg cccggcccat gcggtattac gcaggccaaa gaaagcaagc gaaggtgtca
    36061 ggtcgctcat ttcgacttcg ggccagcgtg gcacaaagac caatggtgaa ctgccatgac
    36121 aggtattggc cccagcagcg gaactcacaa ggcaccataa cgtcccctcc ctgataacgc
    36181 tgatactgtg gtcgcggtta tgccagttgg catcttcacg taaatagagc aaatagtccc
    36241 gcgcctagct ggcggtttgc caaagccgtt gcgactgctg ccagtattgc cagccataga
    36301 gtccacttgc gcttagcatg accaaaatca gcatcgcgac cagcgtttca atcagcgtat
    36361 aaccacgttg tgttttcatg ccggcagtat ggagcgagga gaaaaaaaga cgagggccag
    36421 tttctatttc ttcggcgcat cttccggact atttacgccg ttgcaggacg ttgcaaaatt
    36481 tcgggaaggc gtctcgaaga atttaacgga gggtaaaaaa accgacgcac actggcgtcg
    36541 gctctggcag gatgtttcgt aattagatag ccaccggcgc tttaacgccc ggatgtggat
    36601 cgtagccttc aatctcaaag tcttcgaaac ggtagtcgaa gatggattcg ggtttacgtt
    36661 tgataatcaa cttcggcagc ggacgcggtt cgcggcttaa ttgcagatga gtttgatcca
    36721 tatggttgct gtacaggtgc gtatcgccac cggtccagac aaaatcaccc acttccagat
    36781 cgcactgctg cgccatcata tgcaccagta acgcgtagct ggcaatgttg aacggcaggc
    36841 cgaggaagac gtcacaggag cgctgataaa gctggcaaga gagtttgccg tctgccacat
    36901 agaactggaa gaatgcatgg cacggtgcca gcgccatttt atccagttcg cctacgttcc
    36961 acgctgaaac aataatgcgg cgcgaatccg ggtcgttttt cagctggttc agtaccgtag
    37021 taatctggtc aatatgacga ccatctggcg ttggccaggc gcgccactgt ttaccataca
    37081 ctggcccgag gtcgccattt tcatcggccc attcgtccca gatggtgaca ttgttttcgt
    37141 gtagataagc aatgttagta tcgccctgca ggaaccacag cagttcgtgg atgatggaac
    37201 gcaggtggca acgtttggtt gtcaccagcg ggaatccatc ctgcaggtta aaacgcatct
    37261 gatgaccaaa aatggaaagc gttccggttc cggtacggtc gtttttctgt gtgccttcgt
    37321 cgagcacttt ttgcatcagt tctaaatact gtttcatggt tcctcaggaa acgtgttgct
    37381 gtgggctgcg acgatatgcc cagaccatca tgatcacacc cgcgacaatc atcgggatgg
    37441 aaagaatttg ccccatgctg atgtactgca cccaggcacc ggtaaactgc gcgtcgggct
    37501 ggcggaaaaa ctcaacaatg atgcgaaacg cgccgtagcc aatcaggaac aagcctgaga
    37561 cagctcccat tgggcgcggt ttacgaatat acaggttgag gataataaac agcaccacac
    37621 cttccagcag cagttcgtaa agctgtgatg ggtggcgcgg cagcacaccg taagtgtcga
    37681 aaatggattg ccactgcggg ttggtttgca gcagcaaaat atcttctgta cgggagcctg
    37741 ggaacagcat ggcaaacggg aagttcgggt caacgcggcc ccacaattca ccgttaataa
    37801 agttgcccag acgcccggca ccaagaccaa acggaatgag tggtgcgata aaatcagaga
    37861 cctggaagaa ggaacgttta gtacggcggg cgaagataat catcaccacg ataacgccaa
    37921 tcaggccgcc gtggaaagac atgccgccgt cccagacacg gaacagatac agcggatcgg
    37981 ccataaactg cgggaaattg tagaacagaa cataaccaat acgtcccccg aggaagacgc
    38041 cgaggaagcc cgcatagagt aagttttcaa cttcattttt ggtccagcca ctgcccggac
    38101 gattcgcccg acgtgttgcc agccacattg caaaaatgaa acccaccaga tacatcaggc
    38161 cgtaccagtg aagcgccacg ggtcctattg agaaaatgac cggatcaaac tccggaaaat
    38221 gcagatagct actggtcatc tgtcaccaca agttcttgtt atttcgctga aagagaacag
    38281 cgattgaaat gcgcgccgca ggtttcaggc gctccaaagg tgcgaataat agcacaaggg
    38341 gacctggctg gttgccggat accgttaaaa gatatgtata tgatccgcgc tataaccctc
    38401 cgcgaatcag cccgcccatg ccgcgacgct ccataaaggc tgcaacctga tgacgaactt
    38461 cggtcgccag ttgcgcttcc agactacgct gcgcaagatt ttctgcttcg gcaaaatcaa
    38521 tgcgccgcag caggtatttt acccgcgcta cagaacgtcc gttcatagac agatggcgat
    38581 acccaagccc aatgaggatt gccacgcaca tgggatcgcc cgccatttca ccgcacaaac
    38641 gaagatcgat accatgtatt tccgcttccc gggcgatcat cgccagagct cgtaacattg
    38701 caggatgaag actgtcgtaa atgttcgcca cccgggtatt gttgcgatca acggccagaa
    38761 tgtattgagt cagatcgttg gtgccaacag agatgaaatc gacccgcttt gccagatgcg
    38821 gcagcataaa taccattgac ggcacttcca gcatgatgcc gatacgtggt tttggaattt
    38881 cgtaaccgat catctcctcg acttcacgtc cggcacgttc aatcaggcgg cgtgcttcgt
    38941 caacttcatc gaggctggtg accatcggca acagaatatt caggttgccc gtagcggcat
    39001 tagcacgcag catcgcccgc acctggatca agaagatctc cggctgatcg agcgtaatgc
    39061 gaatcccacg ccaacccagg catggattct cttcgctgat cggcatgtaa ggcagctgct
    39121 tatctgctcc gacatccagc gtacgcaagg tgacgggttt atcattgaac atttgcagca
    39181 tcccctgata ctgcgccacc tgttcttctt ccgacgggaa accactttgc agcatgaatg
    39241 ggatttcagt gcgataaaga ccgatgccat caatacagct gcccagtttt tcttcatgtt
    39301 ccgggcttaa accagcattg agcatgactt ttatacgctc accgcttttt aactgcgcgg
    39361 gtaaattgac gtcatcttcc gccagacggc taagctcaat ctcttcacta attagccgct
    39421 gatattcttg cagcagtacc ggctccggat cgaccagcaa ttcaccgcga tagccatcaa
    39481 cgatcagcgt cctacgatgc agcaccgaag gctgaatatc cgcgcccatc acggtaggga
    39541 tccccagcgc acgtaccatg atcgcagcat gggagttggc tgagccatct cgcacgacaa
    39601 caccgactaa gcgatcctgg ggcagctcag caagagttgt cgctgacagt tcatctgcca
    39661 ccagaatgaa acgttccggc caggcgttcg gcccttgatt agcgtcatca agatgaaaca
    39721 gcaatcgctg acccagcgca cgtaaatcgc cagcccgctc tttgagatag ttatcgctta
    39781 gcgcggcaaa ctgttcggca aatttttcaa tgaccgtttt taccgcccac tctgccaccg
    39841 agcctttatc aacctcggca aacaattcgc gacgcagccg ggtgtccgaa agcaggtgcg
    39901 agtaaagatc gaaaatagcc gccgtttctt tttgtgctcc ggcggcaaag cgtttgctgt
    39961 agcggcgaaa ctcgtttgcc gcttcttcca gcgccccggt cagtcgttcg cgttccagag
    40021 ccggatccag cgttgatgcc tgatacacct gttccattaa aggtaacgtg gcatcctgcc
    40081 agccttcggc aatcgccaca ccaggtgctg ccggtaatgc gcggattcgc gtctggcgat
    40141 attgcccaaa cagggcagtc aactgcgact gagaaagaat agctgccatc tgggtggcaa
    40201 gcgtcaccag gaaggattct tcactttcgt catactggcg caactctcgt tgctgtacca
    40261 ccagtacacc aagcaactgg cgacgttgaa taattggtac gcctaaaaac gcgcggaaac
    40321 gttcttcttt tacggagggg atgtatttga agctggggtg cttttgcgca tctgcaaggt
    40381 ttatcggttc cgccagcctg ccaaccaggc cgacgatccc ttcatcaaac gcgagcgtta
    40441 cagtgcgacc gcgtggtttt ttcagccccc gggtcgccat caggtagtaa caacgtcgat
    40501 catgatcggc caggtagacc gaacagacct cggtatccat cgcaagacag atgtcggtaa
    40561 ccagaatatt taacgcctca ttcaggcgtg gtgcgctggc taccttttcg actatttcgc
    40621 gcaggcgagt gagcataatt ggcgtgactt aacctctttt acgtcgataa gcagatgcgt
    40681 tttgtggttt tggcgtattt tcctgcagtg acatcaccac actcgcgaac tctttcatta
    40741 ccctacggta gacatcacgt ttaaatgaca ccacctgtct gaccggatac cagtaactta
    40801 cccatcgcca gccgtcaaac tctggtgtac tgctggtttg catattgatt tctgcatcgc
    40861 cgcttaccag ctgcaagaga aaccattttt gtttttggcc gatacaaacc ggcttcgtgt
    40921 cccaacgcac caaacgtttc ggtaatttgt agcgcaacca gttacgcgtt gaagcaagga
    40981 ttcgaacgtc tttgcggctt aatcctactt cttcaaacaa ttcacggtac atcgcctgct
    41041 ctgcggattc tccggggttg attccgcctt gcggaaattg ccaggagtgc tgaccaaatc
    41101 gccgggccca cattacctgc ccctggcgat tacaaatcac gatacctacg tttgggcggt
    41161 agccatcgtc atcaatcacc ggactacctc aaaataaagc tttatatacg aatgattgtt
    41221 tcatactcca ggaagacggt aaaccactct ctgcagggca ttacacacta ataacaattg
    41281 aataactcac agttatgtgc agagttataa acagaggaag gggtggatag ccgtttttat
    41341 tcaccttttc tgtggataga gttgtgaaga actacggaat tactacggga aaacccggag
    41401 cattctgaat aagcccgata tagaaatttt aaaatattca ttattagtca atgcattaca
    41461 tgtaatttga gtaatgaaaa agagtaattc gtgacccagg tcacacctct catttacggg
    41521 ttggcgaaag atcaaccaat gccgtattta tccacagaat gtgccactaa gttaagcact
    41581 gaaccactaa aaactggagt ttcgtcgcac gtcaaggctg taaatgaaaa cagtagtgga
    41641 ggtttttcac agttatccca gctttctgtg gataacatgg tgtaagatcc tgtttatttt
    41701 cagtgaccag atttggaaaa cctattgcag tgttgcgcaa ctcgattacc ggcagcctaa
    41761 aaagcaatat aaatcagaga attgaacaac acatgtggaa aaagttacac tgcgaatatt
    41821 cggcacataa ttactgtttg ttttttaacc aaggtatcat gacatgtccc aacctcgccc
    41881 actgctctct cctcccgaaa ctgaagaaca gttgttagcg caagcacagc aactttctgg
    41941 ttatacattg ggagaactgg cggcacttgc cgggctggtt acgccagaga atttaaaacg
    42001 cgataagggc tggattggcg tgttactgga gatctggcta ggtgccagcg cagggagtaa
    42061 acctgagcaa gattttgctg ctctgggcgt ggaacttaaa actatccctg tggatagtct
    42121 tggtcgtccg ctggaaacaa cattcgtttg tgttgccccg ttaacgggta atagcggggt
    42181 gacctgggaa accagccatg tgcgccacaa gctcaaacgc gtactgtgga taccggttga
    42241 aggcgagcgc agcatcccgc tggcgcagcg tcgcgtagga tcaccgttgc tgtggagccc
    42301 gaatgaagag gaagaccgac agctacgcga agactgggaa gaattaatgg atatgattgt
    42361 tctcggtcag gttgagcgga tcaccgctcg tcacggggaa tatttacaga taagaccgaa
    42421 agcagcgaat gcgaaagcgc ttaccgaagc cattggtgcc cggggcgaac ggattctgac
    42481 gctgccacgc ggcttttatt tgaagaagaa tttcaccagt gcactactgg cccgtcattt
    42541 tctgatccag tagccatcgc tttgacctgc cgctttccgg gcatataatt accgcttcat
    42601 ttttttggca gggcttttta gatgttattt gcatggataa ccgatcctaa cgcttggctt
    42661 gcgctcggta cgctgacgct gctggagatc gttcttggga tcgacaatat tattttcctt
    42721 tctctggtgg tggcaaagct tcccacagca caacgtgctc atgcgcgccg tctggggttg
    42781 gcgggagcca tggttatgcg tctggcgctg ctggcatcaa tctcttgggt tacgcgcctg
    42841 acaaatccgc tttttacaat attcagtcag gaaatttctg cccgtgattt gattctgctt
    42901 ctgggtggct tgttccttat ctggaaagcc agcaaggaaa tccacgaatc cattgaaggt
    42961 gaagaagaag ggctgaaaac acgcgtttca tcattcctcg gcgctatcgt gcagattatg
    43021 ctgctggata ttatcttcag cctcgactcg gtgattaccg ctgtgggtct gtcagatcac
    43081 ctgtttatta tgatggcagc cgtggtgatt gccgtaggcg tgatgatgtt cgccgcgcgc
    43141 tcgattggtg attttgtcga acgccatcct tcggtaaaaa tgctggcgct ctctttcctg
    43201 attctggtgg gctttaccct gattctggaa agtttcgaca tccacgtacc gaaaggttac
    43261 atctacttcg cgatgttctt ctctattgcg gttgaaagcc tcaacttgat tcgcaacaaa
    43321 aagaatccgc tctgatactc cgtacgctct cctgcgggag ggcgtactca ctccttttac
    43381 cctcacagtt taagattttc ctgctttcaa gatatataac gtcggtttat aaacagacta
    43441 ttatcatagg taggccaaat gaggataacc ggatgaaaaa atgggcagta ataatttctg
    43501 cagtcggact ggcgtttgct gtttccgggt gttccagtga ttacgtcatg gcgaccaaag
    43561 atggccgtat gattttgacc gatggaaaac ctgaaattga tgatgatacc gggctggtga
    43621 gttatcacga tcagcaaggt aacgcgatgc aaattaaccg tgatgatgtt tcgcaaatta
    43681 ttgaacgtta acaaataagg tcagcatccg gctggcctta agatttttct ctttcccttt
    43741 tcccttccct ctgccatttt tatattcctt atgtcgtgat tataaaaagg aaacggctat
    43801 gcaatatcac cgtatacccc acagttcgct ggaagtcagc acgctggggc ttggcacgat
    43861 gacgtttggt gaacagaaca gcgaagccga cgcccacgca caactcgact atgccgtcgc
    43921 tcagggcatt aaccttatcg acgttgccga aatgtaccca gtacctccgc gccccgaaac
    43981 gcaagggtta accgaaacct acgtcggcaa ctggctggcg aaacatggca gccgcgaaaa
    44041 gttaattatc gcctccaaag tgagcggacc gtcgcgcaat aatgacaagg gcatccgccc
    44101 ggatcaggcg ctggatcgga agaatatccg cgaagcgctg catgacagcc tcaagcgcct
    44161 acagactgat tacctcgatc tttatcaggt gcactggccg cagcgcccga ccaactgctt
    44221 cggcaaactc ggttatagct ggacggattc tgcgcctgcg gtttcgctgc tggatacgct
    44281 ggacgcactg gcagagtacc aacgcgcggg aaaaattcgt tatatcggcg tgtcgaacga
    44341 aactgcattt ggcgtaatgc gctacctgca tctggcggac aaacacgatc tgccgcgtat
    44401 tgtcaccatt cagaaccctt acagtctgtt aaaccgcagt tttgaagtag gtctggcaga
    44461 agtcagccag tatgaagggg tcgaactgct ggcctattcg tgcctgggtt tcggcacgct
    44521 gaccgggaaa tatctcaatg gtgcaaaacc cgctggcgca cgtaatacgc tctttagtcg
    44581 gttcacccgc tatagcggtg agcaaacgca aaaagccgtc gcggcgtatg ttgatatcgc
    44641 cagacgtcat ggccttgatc ctgctcagat ggcgctcgcg tttgtacgct gtcaaccgtt
    44701 tgttgccagc actctgctgg gcgcaaccac gatggatcag ctgaaaacta acatcgaaag
    44761 tttgcatctg gagttaagcg aagacgtatt agctgaaatt gaagcggtac atcaggttta
    44821 tacttatcct gcaccataaa gattaacgcc ggttaaaacc ggcgttaaat attaatgacg
    44881 gcgctgccag atccacagcg ccgttattgc cagcgcaaat agcgcaccga agccaatgcc
    44941 aatgggcacg accgggatgc ctaccattac cgccagcgag taaatgccca gcatcaacaa
    45001 catggcgctg ttttcgccaa ggttttgtac tgcaatcgca ttccccgccc cgacgctttt
    45061 tttaccccgc tcctgtagca acgcattgag cggaacgaca aaaaagcccc ccagcacgcc
    45121 aatcagcatc aacaaggcat aggctggcag cagctcgtgt tgcagggaga aaatcggtac
    45181 caccacgcca atcaaaatcc cggctggcat acagcgtgac acggtttcca gcgtaaccag
    45241 cttcgctgcc gcacctgcgc caaccacgat accaatcact accatcgcgt tgagataggt
    45301 cggcgtagcg ttatcggtaa tgcccagcgc caccggtacc cacagcacca acaggaaacg
    45361 cagcgtgaca cccgctcccc agaataaact ggtgcccacc agcgaaaaac gcgtttcacc
    45421 attgcaccat agcgaggtgc aggcattcag gaaactgcgg gtcatgttga tgagattcca
    45481 ggactgcccc ggacgcgccg ccagtttggg aatgtagata ttggcaacga ccgcaccacc
    45541 gtaggccagt gcgcatgcgg ccagagcgac caggacatgc cagtcagcca gcacaccacc
    45601 ggctacggaa ccgagtaaaa tcgccgctat ggcagaagct tccattaaac cgttagcttt
    45661 tactaactta ctacccgtgg ttaattcgcc gagaataccg tatttcgccg gtgaataggc
    45721 tgcagcacca acacccacca gcgtatagcc gagaaacgga ttgataccaa agcagatact
    45781 ggctgcgccc agcagcttca ggccgttggc aaacatcatc acccggcctt tggcgaagct
    45841 atccgccacc tgcccgacaa acggcgccaa aagaatgtaa gcacctacaa acaccatttg
    45901 caggatgggc tggctccact ccggatagaa ctgcgctttc agtaacgtca gagtggcaaa
    45961 cagtagcgcg ttatcgccaa acgcagagag aaactgcgcc acgataaccg ctttcatccc
    46021 cttcgaccac aacgaagtgt taatgtgcac tgactcactc atcgtgttgt tccgcttcgt
    46081 ctacccagct tttcaacgtg acaaagtcag gtttgccgct gccaagtaat ggcatctgtt
    46141 tcagatagcg aatatcgcgc ggtacagcaa gctccggcac gccgtgctcg cgggcatact
    46201 gttgcaactt atcgcgcgtc agttcgttat ctgtggtgaa aagcaccagt gcctcgcctt
    46261 tgctggcatc gctcttaatc gcagtggcat ggactttatc tggcgaaaca ccaagtgcca
    46321 gttgttccac catttccagc gacaccattt cgcctgcaat tttggcaaag cgttttgcgc
    46381 ggccctgaat ctgcacaaag ccctgctcgt caaaacgcac aatatcgcca gtgtcatacc
    46441 agccgcgctc catttcgccg cgaacattct cggcggtggg cacttccagt acacctggct
    46501 tctccacccg cagatagccg ttcattatgt tcggcccttt cagttgcagg cgtccgccct
    46561 cttcgatacc agggaccgac aacaggcgtg catccattcc tggtagaata cgccctaccg
    46621 taccgggttt cgccgccatc ggtacgttga tagaaacgac aggcgcgcat tcggtcacac
    46681 cgtagccttc aaggatgcgc aggccaaatt tatcctgcca aagctgtttg gtactttctt
    46741 gtagtttttc tgcgcctgcc accacatagc gtagacgata gaagtcatac gggttggcga
    46801 aacgcgcgta gtgaccgagg aaagtcgagg taccgaacaa cacggtgcaa ctgcggtcat
    46861 acaccaagtc cggcacaatg cggtaatgca gcgggcttgg ataaaggaac acttctgcac
    46921 ctgtaagcag tggcgtaaac aggcctaccg tcagcccaaa ggagtgaaac agcggtaatg
    46981 ccgacataaa gcgatcgttg gtggtgaagt cggcaatcgt tttaatctgc tcgacgttcg
    47041 ccagaatgct tttatggcta tggacgacgc cttttggatg gccttcagaa ccggaggtaa
    47101 agaggatcag cgcctcttct tccggctgct gttttacctg cgccaggcgc ggcatcagca
    47161 aatgagcgaa gatccatact ttgtcggcag tggtgacatc tgcttttaaa tcttccagat
    47221 agacccagcg cacctgagta agttgctccg gcagatgcca gagtttgcct ttatcgagaa
    47281 actggcggga agtgaagatg gttttgattt cagccgccgt aatagcactg gtcagccctt
    47341 ttaccccggc agtgtagttc atcattgcgg gaatgcggcg acgcgcgatg gccccaaaaa
    47401 tcactgccgc actgatgcct gcattgggca gcattaagcc gatgcgttcg ccttcaacac
    47461 tgtatttttc aaggatgcgt ccaacaaaca gcgttttcgt aagcaatttg cgataggagt
    47521 ctggggtaaa gttgacgtct tcgacacatt tcttcccggc tccgaagcgg tacattgcac
    47581 tcagtaaaga ttcgtacaga gtttcacgcg ggcgcaccgc catacgcgct tccatcatta
    47641 tttgatgcag catttcgcca gcaattttgc gacggtcacg ggcacgcggc gcatccggca
    47701 tcgccacctg cgttggaggc aaaatatgca gagtaatttg cgggaacaag cgacgtttaa
    47761 ccagaccttt caggcggctg aagtgcgtaa gttccgcccc ttcaatacgc acaggaataa
    47821 ccgttgcacc agacttcgcc gcgacaaaac ccgcgccatc gtagattttc atcagcgagc
    47881 ctgtagtggt gatgcgtcct tcagggaaaa tcaccactgg tcggccctgt tccaccagac
    47941 gtaccagatg tttaatagcc ataggttgcg tcgggtcgag aggaacaaag tcgataaatg
    48001 atttcagcca acgcatatac cactgttggc ttattgaggt gtaaacggca aacactggac
    48061 gcacaggtaa aaacagtcca agcaaaatgc catcaataaa agagacgtga ttaggcgtaa
    48121 ttagtacgcg ctcgcccttc agtgcctggg tgtcacccgt aacgcgaacg cgatacaaaa
    48181 cacggcacaa atttcgaaaa aagctaaaaa gcattccaac tccctttgct ctgattcagt
    48241 aaaagcgaat ggagggagat tacacgagat aaagaacgcg agcgacagta aattaggtgc
    48301 gaaaaaaaac ctgcgcatcc gcgcaggttg gtgcaagaga cagggtacga agagcgtacc
    48361 gaataatctc accaatcaat acctctggga tcttgattgt ggtctgcacg acgactcttc
    48421 gccagcgaga aaacgcaaag gaatgaaggg aaatgcaacg aggtgtgtaa attgtcggtt
    48481 actgttacag attgatgacc ggcaaaaaaa aacctgcgca tctgcgcagg ctggtgtaat
    48541 tcatgtgctc aacacgaagt tgacttcacc tatcaatacc tctgggatca ccactttagc
    48601 aacctgaagc caaacgccac cagcggtcaa tcgcaacagc gtttcgcaaa gtgtaaccaa
    48661 aggtttgcaa tctctttttt tgtcttactt atttgcgttt ggctcacatt cccacgatga
    48721 aaacacgcca ccccttgaac caacgggcgt tttccgtaac actgaaacaa tgtaagcgtt
    48781 tacccactaa ggtattttca tggcgaccat aaaggatgta gcccgactgg caggcgtttc
    48841 agtcgccacc gtttcccgcg tcattaataa ttcacccaaa gccagcgaag cttcccggct
    48901 ggctgtgcat agtgcaatgg agtctcttag ctatcacccg aacgccaacg cccgtgcgct
    48961 ggcgcagcag accactgaaa cggtcggtct gatcgttggt gatgtttccg atccgttttt
    49021 cggtgcaatg gtgaaagcgg tcgaacaggt ggcttatcac accggtaatt tcttattgat
    49081 tggcaacggt taccacaacg aacaaaaaga gcgtcaggct attgagcaac tgatccgcca
    49141 tcgctgtgct gcgttggtcg tccatgccaa aatgatcccg gatgccgatt tagcctcatt
    49201 aatgaaacaa atgcccggta tggtgctgat caaccgtatc ctgcctggct ttgaaaaccg
    49261 ttgtattgct cttgacgatc gttacggtgc ctggctggca acgcgtcatt taattcagca
    49321 aggtcatacc cgcattggtt atctgtgctc taaccactct atttctgacg ccgaagatcg
    49381 tctgcaaggg tattacgatg cccttgctga aagtggtatt ccggccaatg atcggctggt
    49441 gacatttggc gaaccagacg aaagcggcgg cgaacaggca atgaccgagc ttttgggacg
    49501 aggaagaaat ttcactgcgg tagcctgtta taacgattca atggcggcgg gcgcgatggg
    49561 cgttctcaat gataatggta ttgatgtacc gggtgagatt tcgttaattg gctttgatga
    49621 tgtgctggtg tcacgctatg tgcgtccgcg cctgaccacc gtgcgttacc caatcgtaac
    49681 gatggcgacc caggctgccg aactggcttt ggcactggcg gataatcgcc ctctcccgga
    49741 aattactaat gtatttagtc cgacgctggt acgtcgtcat tcagtgtcaa ctccgtcgct
    49801 ggaggcaagt catcatgcaa ccagcgacta accgcagtta aagcaattcc agcgccagta
    49861 attcttcgat ggtctggcga cggcgaatca accgcgcctg accattatca aacagaactt
    49921 ctggtaacag cggacggcta ttgtagttgg atgacattga tgcgccatat gcccctgtat
    49981 catgcagtac cagataatca cctgccttca cttccggcaa ggcgcgggtt tcaacatttc
    50041 ccccttcctg ctgggtaaag acatcgcccg attcacataa cggcccggcg acgacggttt
    50101 ccaccgttgg cgcgtgttcc agagaacgac catcagctgc cagggcactg atatggtggt
    50161 aactaccgta cattgccggg cgcatcagat cgttgaaccc ggcatcaacc agcacaaagt
    50221 ggcggctacc catttgtttg acgctccgca cctgagtaat taatacgcca gactgcgcta
    50281 ccaggaagcg acccggttca atttccagtt tcacagggtg gcccaaatgg cgagcgattt
    50341 gctcacgcgc ggcattccac agaccataat aatgttcggt atcaaccgcc tcttcaccct
    50401 gttgataagg aatagaaagc ccaccgcccg cagaaatagc ctgtaaatcc tgaccgaatt
    50461 cgataacctg acgcaccata gcaccacaca cctgctccag atgggcataa tcaacgccag
    50521 aaccaatgtg catgtgaatg ccgactagct gcagatgatg acgttgtatc acgtccagtg
    50581 cggcgggcag atcggtgtac cagataccgt gcttgctgtt ttcgccaccg gtattggttt
    50641 tttggctatg tccgtgacca aaccccggat taacgcgcag ccatacccga tgccctggcg
    50701 aaacctggcc cagttggtcg agcgtatcaa cagaacccgc attcaccgga atttgcaatt
    50761 cactgacgcg ttcaagcgtc gcctgatcga taacatctgc cgtaaaaaca atatcatcgg
    50821 ggtgcgtttg cggattgtaa cccgccgcca acgcacgctc tatttcgcct aacgagacgg
    50881 aatccacttt cacgccctgc tcacgcatta agcgcaaaat atgaatattg gaacaggctt
    50941 tctgtgcaaa gcgcaccact tcaaactgtt tcagcgctgc aatctgccga cgaataattt
    51001 gcgcatcgta gacccacacc gggcagccaa attcagcggg caaacgcagc agattttcgg
    51061 cggtgagatc ggtatcggtg ctgaacagtg aatgtggcat aacaaactcc agatgagtgc
    51121 ttttttatga ttacgccaca tcataaaaag aataaaaaat atcgatttat gtcgagtcta
    51181 tgcaaaaatg atatggatta ccggattgcg agagagcgct aatggccgcc gttaacttac
    51241 gtcatattga aatttttcat gcggtaatga ccgccggaag cctgactgag gcagcacacc
    51301 tgctacacac ctcacagcca accgtcagcc gcgaactggc gcgctttgag aaggtgatcg
    51361 ggctgaaatt gtttgagcgc gtacgtggac gattacatcc taccgtgcaa ggactgcgtc
    51421 tgtttgaaga agtgcaacga tcctggtacg gactggatcg cattgtcagc gccgcagaaa
    51481 gtctgcgcga gtttcgccag ggagaactgt ctattgcctg cctgccggtc ttttcgcaat
    51541 cttttttacc gcagctcctg caaccctttc tggcacgtta tcccgatgtc agcttaaata
    51601 tcgtgcccca ggaatcaccg ctacttgaag agtggctctc ggcccagcgt catgatttag
    51661 gactcactga gacactccat acgcctacgg gaacagaacg taccgaatta ctctctttag
    51721 atgaagtgtg tgtgttacct ccgggtcatc cgctggcggt aaaaaaggta ttaacgccgg
    51781 atgattttca gggtgagaat tacatcagcc tttcccgtac tgacagctat cgccagttgc
    51841 tggatcagct atttactgaa catcaggtta aacgacgcat gatcgtagaa acccacagcg
    51901 ccgcgtcagt ctgcgcaatg gtacgggcgg gggtaggtat ttcggtggtt aacccgctca
    51961 ccgcactaga ttatgcggca agcggtttag tggtgcggcg gttcagtatt gcggttccgt
    52021 tcaccgtcag cctgatccgc cccctgcacc gcccgtcatc agcgctggtt caggcgttta
    52081 gtgggcattt acaagcgggg ttaccgaaac tggtcacttc tcttgacgct attttgtcgt
    52141 cagctacgac agcataaaag cgacagcatc ctcggcatgg atcgccgcgg tatcaaacac
    52201 aggcaaaaca ctgcgctctt ctggcaccag taaaccaatt tctgtgcagc caaaaatgac
    52261 gccctgtgcg ccctgttctg caaggcgagc aatcacttgc gcataataag cgcgtgacgc
    52321 ttcggtaaat tgccccagac acagttcttc aaaaataatc tgattaattt tcgcccgttc
    52381 atccgcttca ggaataagac aattgatgga aaattgttcc gtcagccgcc cgcgataaaa
    52441 atcctgttcc atggtgtaac gcgtacccag cagcgccaca cgagtcattc cggccccggt
    52501 aattgcacgt ccggtggcat ccgcaatgtg taagaaaggc agagtgcaac gtgactcaat
    52561 ggcatccgcc actttatgca tcgtattggt acataacaca ataccttctg cgcccgcccg
    52621 ctgtaagcca agcgccgcct cagccagaat gtccccggtt ttatcccatt ccccgcgacg
    52681 ctggcactct tctatttcat gaaaatcgac gctatgtagc agcatttgcg cagagtgaag
    52741 cccaccaagc cgctgtttaa tgccttcatt tatcagacga tagtaaggaa tggtggattc
    52801 ccagctcatt cctcccagca aaccaattgt tttcattcct ctctcctgat gtgtgttacc
    52861 gcaacaaggc tgaataagga acgagacaaa cgcctcaacg gccaggtgcc caatctctat
    52921 taacgaaaaa agggccggat gtacagcaca tccggcccgt gaaatcagac gccgatattt
    52981 ctcaacttct cgcctgccat cagtttgcgt tcgatatgtt ccagcgtgac atttttggtt
    53041 tccggaatga gccagaaagt aatacccaca aacgcaatgt tcagcgcagt gtagagccag
    53101 aacgtaccgg cagcgccaat gctatcaagc agtgtcagga aggccgcgcc gataatcata
    53161 ttcgacaccc agtttgtcgt cgtcgaacag gtaataccga aatcgcggca tttcagcggc
    53221 tgaatttcag agcacaggat ccacaccact ggcgcggcgc tcatcgcata accggcaata
    53281 cacatcatcg tcatgccaac agagagccag gacaagccac tggaagccgt accgttatca
    53341 aactgcatca ggcaatagcc cagcaccaga gtgcctaacg ccatcacgct gaaaccaatt
    53401 ttcagagccg gtttacgacc tgctttatct accgtaaaga ccgcaatgaa ggtcgcgaac
    53461 ataaaggtca gtcccacgac cagagtcgca atcatctgtt gttctgtggt cgtaaagccc
    53521 gccattttga agatacgcgg cgcgtagtac atgatgatgt tcataccggt aaactgctgc
    53581 atcgcctgca acaacatacc gagaaacaca gcacgacgga cgttacggtt gatcttaaac
    53641 agtgcccaac cgccctgttt taacttcagg ctttcacgaa tttcgttgag ttcttctcgc
    53701 gctttttccg acgtatcgcg cagcatacgc aacacttctt ccgcctcaat atgacgcccc
    53761 ttttccgcca gccagcgcgg gctatttggc aggaagacca ccagaataat cagcagaact
    53821 gctggtaaag caagaccccc caacattgcg cgccagttac cgctataact gaacgctgta
    53881 tcggataaaa acgccagcac gatgccgagt gtgaccatca actggtacat actgatcatc
    53941 ttaccgcgaa cgttttcact tgccatttca gaaagataca gaggagcggt gtaagacgcg
    54001 atcccgacag caatgcccag caccacacga gcggcgatta acatctctac gctggtcgca
    54061 aaagcggacc ctatagaacc gagtacaaac aggatggccc ccgccatcag gctgtattta
    54121 cgccccaggc ggaacgacag ccaaccatta aacagcgcac caattgctgc gccgagcatc
    54181 atgctactaa ccacccattc ctgcaaacga ctggtcagca caaagtgatc ggtaatgaac
    54241 ggcaacgctc cggcgattac gccgatatca agaccaaata acaatcctgc gaccgcagca
    54301 gctaccgaaa caaacatatt catacgccgc gtatcacgca aagaacgtgg cgttaaagca
    54361 gattccgtat tgatagtaac cattttttcc tgccagcaga gagtaagaca ttgtgaaaaa
    54421 atacgtgaac aattcacaca ggtgtcaggt cggaaacagc ataaatatgg attaaattgc
    54481 tgcgacatgc cgttatgtga tggatattcc aattttaaaa ttaagttgaa ttattgagat
    54541 tattattaac ctcctaattt taaagcagat aaaattcata aagttcatta attgataatt
    54601 gatatggatt atttcataac catgatatgg attatgatga tctagagata taaaaaaccc
    54661 tgccatgcgg cagggtcata aaagtaagag gaatgaatta acgcgccagc caaccgccat
    54721 ccacagcaat ggcataacca tttacataat ctgaagcgct ggaggcaaga aacactaccg
    54781 gccccatcag gtcactcggc agtccccaac ggccagctgg aatgcgatcg agaatttccg
    54841 cgctacgttg ttcatctgcc cgcagttgtt gggtattgtt ggtcgccatg taacccggag
    54901 ctatcgcatt aacattaatg ttgtgtttag cccattcgtt tgccatcaat cgcgtcacac
    54961 ccatcacgcc gctttttgat gcggtataag aaggcacacg gatcccgccc tggaaggaga
    55021 gcattgacgc gatattgata atcttgccgc cattgccttg cgcgataaag tgtttcgccg
    55081 ctgcctgaga catgaagaat acgctcttga tattcaggtt catgacatcg tcccagtctt
    55141 tttcgctgaa ctcgagagcg tcttcgcggc gaatcaatcc ggcgttattc accaggatat
    55201 caatatgacc aaattcagct accgcgcgat ccagcagtcc aggaatgcca tcaatctttc
    55261 gcagatcggc ggtcaggctt aaaaaacgac gcccctgcgc cgtgacctgc tcgatggttt
    55321 cagtcggttc aacgatgtta atgccaacaa tgtcacagcc cgcttgcgcc agccccaacg
    55381 ccatcccctg gcccagccca gtatcacaac cagtgacgac cgcaacttta ccttggagag
    55441 aaaatgcact taaaatcata acaacacctt attcgtttat gcccacaact agcgcaaatc
    55501 tttaacgccc acatggtcca tatcattaaa gacctggttt tcaccgacca tgccccagat
    55561 aaaggtataa gctttggttc cgacgccgga atggatcgac cagctcgggg agatcaccgc
    55621 ctgctcgtta tgcatcacaa tgtgacgcgt ttcttgcggc tgccccatca tgtggaaaac
    55681 gcaggcgtca tcatccatat tgaaatagaa ataaacttcc atccggcgct cgtgggtgtg
    55741 acacggcatg gtgttccaca agttacctgg agccagctcc gtcagcccca tactcaattg
    55801 acaggtttcc agtacatccg ggacaaaata tttgttaatc gtgcgacggt tactggtgag
    55861 gttatcgcct aacgtgactg gagatacttc gtccggtgtg acttttttgg tgggatacgt
    55921 cgtatgcgcg ggtgcgcaat tgtaataaaa cttcgccgga gtggcggtat caatactggc
    55981 aaagacaact tcttttgcac ctttaccaac atacagggcg tcgcggtgac cgatttcata
    56041 gcattgacca tcgacagtaa tcgtaccggc accgccaata ttgataacac ctaactcgcg
    56101 acgttcaagg aaatagctta cgccgagttg tttaccaact tccccgccaa cggaaaccgt
    56161 tttagttatc ggcataatgc cgccaacaat aattcggtca atgtggctgt aaaccatggt
    56221 gtactcatcg gcgacaaata ccttttcaac caaaaattca ttgcgcagcc cttgggtatc
    56281 cagcgttttt gcgtgcgcac cgtggatgct ctgtcttacg tccacatcaa cctccgataa
    56341 tcagtgaagc gtgaaaatag aaaacgatag tgaaacactg tttcgttttc tctataaagg
    56401 gatgatatgc ccatgggcta gggttttcaa ttaaaaataa aacaatgttt tatttttaat
    56461 ttaaggatta aaaaaagtgc agtgtcgatc acgaatatgt catgagaatg gaaaaatgag
    56521 ggagcccaga aaatgtatgt cgacttgcca gccatcatta ttcaacagat taatgacggc
    56581 agatatttat cggtaaggag gaaatgcaga gctactcgtc acgttcaatg gtcaatgcca
    56641 cgccctggcc cccgccgata caaagcgttg ccagcccttt gcgggcatta cgtttcacca
    56701 tttcatgaac cagagaaacc aggattcggc aaccggaagc gcctatcggg tgaccgagtg
    56761 cgatcgcgcc accattgaca ttgacccgac gctcatccca ctcaagcatc ttgccaaccg
    56821 aaagcgcctg tgcagcaaac gcttcattag cctcgataag atcgacatca gccaactgcc
    56881 agcctacacg ctccaggcaa cggcgggtcg catacaccgg cgcaattccc atcaatgccg
    56941 gatctacacc aacgctggca aatgcgcgga tgcgggccag cacgggtaaa ttcaacgctc
    57001 gtgctttggc ttcgctcatc atcattaccg cagctgcgcc atcgtttatg gatgatgcat
    57061 tccccgcggt cacagaaccg agactatcaa atgaaggatt taaacgggct aaaccttctg
    57121 cactggcgtc agtgcgtggc tgttcatcgg tatcaacaac caacgtctga ccgttacttt
    57181 ggatcattac cgggacgatc tcatctttaa atcgtccggc gtcaatcgcc gctcgcgctt
    57241 tttgttgcga gctaagtgcg taagcatcct gcaactgacg gctgatgcca tattcgcgag
    57301 ccagattttc ggcggtgaca ccaatatgat aatcattgaa ggcatcccac aacccatcat
    57361 gcaccagact gtcaaccaac tggctattgc caagctgtgc accggtgcgg ctatcagtca
    57421 gaacatgtgg tgcgcggctc atgttttcct ggccaccggc gatgacaata tcagcctcgc
    57481 cacactgtat cgcctgagta gccagatgca gtgctttaag accggaaccg caaacgtcat
    57541 taatcgtgat tgcagaaacg ctgttaggaa gaccaccttt aatagccgat tgccttgccg
    57601 gattctgccc tgcccctgca gtcaacacct gaccaagaat tacttcatcc accgcatatg
    57661 caggaacgcc ggtacgttct attaacgctt tcacgaccag actaccaagt tccacggcgg
    57721 aatgacccgc taacgcacca cgaaagcagc cgataggtgt ccgtaacgcc ccgacaatca
    57781 caacgtcttt catcacaacc tcgcaccaat taacagtgca atagtaaatg attgttaatt
    57841 acagttatct taattgttta aaaaaagtga tttttatcac aaaggaaata tgcctgagca
    57901 gtagtcagag tcataactgg cacgtaaggt ttgcaaccac taacccacca atagatgggt
    57961 agatagggcg ttaatctccc atacttaact tggtttatgg taaattgccc tccattttgt
    58021 ttaatttgta gatgatacgt tcagataatg tctaatattt ggtctaaaga agaaactctg
    58081 tgaagtttcg cgctctatgg cacagccgtt gatgcaggca cgctattcct tcctattcag
    58141 ttaggttcgg caggggctgt ggtcctgttt attactgctc tggtcgcctg gcctttaaca
    58201 tattggccac ataaagcctt atgccagttc atcctctcat caaaaacatc agcaggtgaa
    58261 gggataacgg gcgcggtaac acactactat ggcaagaaga ttggtaatct gattaccacg
    58321 ctgtacttca tcgccttttt tgtcgtcgtg ttgatatatg cagtggcaat taccaactca
    58381 cttacggaac agctggcaaa gcatatggtt attgatcttc gcatccgtat gttggtgagt
    58441 ctgggtgttg tattaattct gaatctcatt tttctgatgg gacgtcatgc cactattcgg
    58501 gtaatgggat ttttggtatt cccattgatt gcctatttct tatttctttc catttacctt
    58561 gtcggtagtt ggcaacctga tctattaaca acccaggtag agttcaatca gaataccctt
    58621 caccagatat ggatatcgat tcccgtgatg gttttcgcct ttagccatac gcccattatt
    58681 tctacgtttg ccatagacag acgtgaaaaa tatggcgaac acgctatgga taaatgcaaa
    58741 aaaattatga aagtcgctta tctcatcatc tgcataagtg tactgttctt tgtctttagc
    58801 tgcctgcttt ctattccatc ttcgtatatt gaagcggcta aagaagaagg ggtcaccatt
    58861 ttatcggcgc tttctatgct gccgaacgcc ccagcatggt tgtcaatttc cgggattatt
    58921 gtcgcagtag ttgcgatgtc gaaatcattc ctgggtacgt actttggcgt tattgaaggt
    58981 gccacagagg tcgtcaaaac aacactacag caggttggtg taaagaaaag tcgtgcattt
    59041 aaccacgcac tatcaattat gttggtatcg ctgattacct tcattgtttg ttgcattaac
    59101 ccgaacgcga tttcgatgat ttacgcgatc agcggcccgc tcattgccat gatacttttc
    59161 atcatgccta cgctgtcaac gtatctcatc ccggcgctta aaccctggcg ttccatcgga
    59221 aatctgatta cgctgatcgt gggtatcctg tgcgtatcgg taatgttctt tagctaatat
    59281 ccgttgtgga tggttcaaaa ttgctacaat tttgaaccat tccatttttc ataatattca
    59341 tttgttatta ttccgcccta tcacttctct cgtcatatcc tagaattatc agttagttaa
    59401 acacacaaag agatctgaac atcttaaata agactattta agatgcataa cttagattcg
    59461 caagatatct tctggtcatt caaaaacaat ttgcgatata gatattctca taccatgaga
    59521 tagttatctg gaccacgttc gaccatgcaa gattaaatca gtgaaacata atcatattta
    59581 ataacgagaa atgcattttt aaatatctta atcagaatta tagcaaatac agattaatcg
    59641 caatttttcc cagcaaaatt acaaaatcaa ggtgtattga catcatcaat caccaatgaa
    59701 attatgcaat tatatacgga tagggaggtt cttaacatgg ggctttgtag tcgttataaa
    59761 agtcttacat gcaatagttg ctcaatgcat tgccaaataa tgccagaaga gtcaccgcgt
    59821 ttgcagtact gcgcaaactc gtgttttgat cttacccagc aatagtggac acgcggctaa
    59881 gtgagtaaac tctcagtcag aggtgact
//
LOCUS       DAJTQY010000013        59551 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.013, whole genome shotgun sequence.
ACCESSION   DAJTQY010000013 DAJTQY010000000
VERSION     DAJTQY010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 59551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59551
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.013"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(32..2479)
                     /gene="glgP"
                     /locus_tag="OO349_000820"
     CDS             complement(32..2479)
                     /gene="glgP"
                     /locus_tag="OO349_000820"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HCR5865994.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(2498..3931)
                     /gene="glgA"
                     /locus_tag="OO349_000821"
     CDS             complement(2498..3931)
                     /gene="glgA"
                     /locus_tag="OO349_000821"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HCR5865995.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KSQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(3931..5226)
                     /gene="glgC"
                     /locus_tag="OO349_000822"
     CDS             complement(3931..5226)
                     /gene="glgC"
                     /locus_tag="OO349_000822"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HCR5865996.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(5244..7217)
                     /gene="glgX"
                     /locus_tag="OO349_000823"
     CDS             complement(5244..7217)
                     /gene="glgX"
                     /locus_tag="OO349_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HCR5865997.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGNSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPSGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLNWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITVVWQGPAHGLCVFQR"
     gene            complement(7214..9400)
                     /gene="glgB"
                     /locus_tag="OO349_000824"
     CDS             complement(7214..9400)
                     /gene="glgB"
                     /locus_tag="OO349_000824"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HCR5865998.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFCINQPGKWREILNTD
                     SIHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(9673..10776)
                     /gene="asd"
                     /locus_tag="OO349_000825"
     CDS             complement(9673..10776)
                     /gene="asd"
                     /locus_tag="OO349_000825"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCR5865999.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            10968..11561
                     /gene="yhgN"
                     /locus_tag="OO349_000826"
     CDS             10968..11561
                     /gene="yhgN"
                     /locus_tag="OO349_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HCR5866000.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(11618..12958)
                     /gene="gntU"
                     /locus_tag="OO349_000827"
     CDS             complement(11618..12958)
                     /gene="gntU"
                     /locus_tag="OO349_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCR5866001.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILYETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGAATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(12962..13489)
                     /gene="gntK"
                     /locus_tag="OO349_000828"
     CDS             complement(12962..13489)
                     /gene="gntK"
                     /locus_tag="OO349_000828"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HCR5866002.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDSAFAMQRTNKVSLIACSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(13628..14623)
                     /gene="gntR"
                     /locus_tag="OO349_000829"
     CDS             complement(13628..14623)
                     /gene="gntR"
                     /locus_tag="OO349_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HCR5866003.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(14847..15542)
                     /gene="yhhW"
                     /locus_tag="OO349_000830"
     CDS             complement(14847..15542)
                     /gene="yhhW"
                     /locus_tag="OO349_000830"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HCR5866004.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSCANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(15665..16702)
                     /locus_tag="OO349_000831"
     CDS             complement(15665..16702)
                     /locus_tag="OO349_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR5866005.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKTFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAN"
     gene            17035..17523
                     /gene="yhhY"
                     /locus_tag="OO349_000832"
     CDS             17035..17523
                     /gene="yhhY"
                     /locus_tag="OO349_000832"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR5866006.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            complement(17582..19324)
                     /gene="ggt"
                     /locus_tag="OO349_000833"
     CDS             complement(17582..19324)
                     /gene="ggt"
                     /locus_tag="OO349_000833"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HCR5866007.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDIKKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEA
                     TNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYG
                     ASDPRSVDDLTAGY"
     gene            19444..19884
                     /locus_tag="OO349_000834"
     CDS             19444..19884
                     /locus_tag="OO349_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HCR5866008.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            complement(19871..20614)
                     /gene="ugpQ"
                     /locus_tag="OO349_000835"
     CDS             complement(19871..20614)
                     /gene="ugpQ"
                     /locus_tag="OO349_000835"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCR5866009.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QHAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(20611..21601)
                     /locus_tag="OO349_000836"
                     /pseudo
     CDS             complement(20611..21601)
                     /locus_tag="OO349_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate import ATP-binding
                     protein UgpC"
     gene            complement(21603..22448)
                     /gene="ugpE"
                     /locus_tag="OO349_000837"
     CDS             complement(21603..22448)
                     /gene="ugpE"
                     /locus_tag="OO349_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HCR5866010.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            complement(22445..23332)
                     /gene="ugpA"
                     /locus_tag="OO349_000838"
     CDS             complement(22445..23332)
                     /gene="ugpA"
                     /locus_tag="OO349_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HCR5866011.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVMLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSMVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            complement(23430..24746)
                     /gene="ugpB"
                     /locus_tag="OO349_000839"
     CDS             complement(23430..24746)
                     /gene="ugpB"
                     /locus_tag="OO349_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HCR5866012.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAVFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYSAKLKASGIKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            complement(25143..25856)
                     /gene="livF"
                     /locus_tag="OO349_000840"
     CDS             complement(25143..25856)
                     /gene="livF"
                     /locus_tag="OO349_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HCR5866013.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            complement(25858..26625)
                     /gene="livG"
                     /locus_tag="OO349_000841"
     CDS             complement(25858..26625)
                     /gene="livG"
                     /locus_tag="OO349_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HCR5866014.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            complement(26622..27899)
                     /gene="livM"
                     /locus_tag="OO349_000842"
     CDS             complement(26622..27899)
                     /gene="livM"
                     /locus_tag="OO349_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HCR5866015.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWMFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLVVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAVLLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            complement(27896..28822)
                     /gene="livH"
                     /locus_tag="OO349_000843"
     CDS             complement(27896..28822)
                     /gene="livH"
                     /locus_tag="OO349_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HCR5866016.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYCVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            complement(28870..29979)
                     /gene="livK"
                     /locus_tag="OO349_000844"
     CDS             complement(28870..29979)
                     /gene="livK"
                     /locus_tag="OO349_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HCR5866017.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINVKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            30403..30786
                     /gene="panM"
                     /locus_tag="OO349_000845"
     CDS             30403..30786
                     /gene="panM"
                     /locus_tag="OO349_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HCR5866018.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            complement(30985..32088)
                     /gene="livJ"
                     /locus_tag="OO349_000846"
     CDS             complement(30985..32088)
                     /gene="livJ"
                     /locus_tag="OO349_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HCR5866019.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEELLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(32359..33213)
                     /gene="rpoH"
                     /locus_tag="OO349_000847"
     CDS             complement(32359..33213)
                     /gene="rpoH"
                     /locus_tag="OO349_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HCR5866020.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMHGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            complement(33458..34516)
                     /gene="ftsX"
                     /locus_tag="OO349_000848"
     CDS             complement(33458..34516)
                     /gene="ftsX"
                     /locus_tag="OO349_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HCR5866021.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            complement(34509..35177)
                     /gene="ftsE"
                     /locus_tag="OO349_000849"
     CDS             complement(34509..35177)
                     /gene="ftsE"
                     /locus_tag="OO349_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HCR5866022.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            complement(35180..36676)
                     /gene="ftsY"
                     /locus_tag="OO349_000850"
     CDS             complement(35180..36676)
                     /gene="ftsY"
                     /locus_tag="OO349_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HCR5866023.1"
                     /translation="MAKEKRRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     AKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVAEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEDKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            36826..37422
                     /gene="rsmD"
                     /locus_tag="OO349_000851"
     CDS             36826..37422
                     /gene="rsmD"
                     /locus_tag="OO349_000851"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HCR5866024.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRVVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQHEAQGESDAD"
     gene            37412..37681
                     /locus_tag="OO349_000852"
     CDS             37412..37681
                     /locus_tag="OO349_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HCR5866025.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(37684..38043)
                     /locus_tag="OO349_000853"
     CDS             complement(37684..38043)
                     /locus_tag="OO349_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HCR5866026.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            38184..38810
                     /locus_tag="OO349_000854"
     CDS             38184..38810
                     /locus_tag="OO349_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HCR5866027.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            38884..41082
                     /gene="zntA"
                     /locus_tag="OO349_000855"
     CDS             38884..41082
                     /gene="zntA"
                     /locus_tag="OO349_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HCR5866028.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAT
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(41184..41429)
                     /gene="tusA"
                     /locus_tag="OO349_000856"
     CDS             complement(41184..41429)
                     /gene="tusA"
                     /locus_tag="OO349_000856"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HCR5866029.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            41650..42315
                     /locus_tag="OO349_000857"
     CDS             41650..42315
                     /locus_tag="OO349_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HCR5866030.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            42388..42945
                     /gene="dcrB"
                     /locus_tag="OO349_000858"
     CDS             42388..42945
                     /gene="dcrB"
                     /locus_tag="OO349_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HCR5866031.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(42949..44166)
                     /locus_tag="OO349_000859"
     CDS             complement(42949..44166)
                     /locus_tag="OO349_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5866032.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            44300..45349
                     /locus_tag="OO349_000860"
     CDS             44300..45349
                     /locus_tag="OO349_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR5866033.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLALM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            45404..45991
                     /gene="acpT"
                     /locus_tag="OO349_000861"
     CDS             45404..45991
                     /gene="acpT"
                     /locus_tag="OO349_000861"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HCR5866034.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            46102..47676
                     /gene="nikA"
                     /locus_tag="OO349_000862"
     CDS             46102..47676
                     /gene="nikA"
                     /locus_tag="OO349_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HCR5866035.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALNKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            47676..48620
                     /gene="nikB"
                     /locus_tag="OO349_000863"
     CDS             47676..48620
                     /gene="nikB"
                     /locus_tag="OO349_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HCR5866036.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGSWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            48617..49450
                     /gene="nikC"
                     /locus_tag="OO349_000864"
     CDS             48617..49450
                     /gene="nikC"
                     /locus_tag="OO349_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HCR5866037.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWQPYDPQAIDLPSC
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            49450..50214
                     /gene="nikD"
                     /locus_tag="OO349_000865"
     CDS             49450..50214
                     /gene="nikD"
                     /locus_tag="OO349_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HCR5866038.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPYALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            50211..51017
                     /gene="nikE"
                     /locus_tag="OO349_000866"
     CDS             50211..51017
                     /gene="nikE"
                     /locus_tag="OO349_000866"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HCR5866039.1"
                     /translation="MTLLNVCGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWHGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQLV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIAETQAVGDKLTFSSDAGRVLQNAVLPAFPVRRRTSEKV"
     gene            51023..51424
                     /gene="nikR"
                     /locus_tag="OO349_000867"
     CDS             51023..51424
                     /gene="nikR"
                     /locus_tag="OO349_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HCR5866040.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(51544..51903)
                     /locus_tag="OO349_000868"
     CDS             complement(51544..51903)
                     /locus_tag="OO349_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HCR5866041.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(51900..52175)
                     /locus_tag="OO349_000869"
     CDS             complement(51900..52175)
                     /locus_tag="OO349_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HCR5866042.1"
                     /translation="MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEI
                     KKGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVCDWLLSIGVKP"
     gene            complement(52234..53358)
                     /locus_tag="OO349_000870"
     CDS             complement(52234..53358)
                     /locus_tag="OO349_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5866043.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            complement(53358..56093)
                     /gene="rbbA"
                     /locus_tag="OO349_000871"
     CDS             complement(53358..56093)
                     /gene="rbbA"
                     /locus_tag="OO349_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HCR5866044.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVVVLGGDMHDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGLGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            complement(56090..57157)
                     /locus_tag="OO349_000872"
     CDS             complement(56090..57157)
                     /locus_tag="OO349_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR5866045.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            complement(57523..59145)
                     /locus_tag="OO349_000873"
     CDS             complement(57523..59145)
                     /locus_tag="OO349_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCR5866046.1"
                     /translation="MKIGTVAGTNGSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     NNCYQFIVTINSKDYGCPLDNTDINWCSIVYLLNNMTVNDNANDVAVTESYKPIWNWE
                     ISQYNVFDIKFETIIKPQFADRTYFSNCSPVDPTSTKPTYFGDTDGSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNTIISILNRRDNVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDQFIIDLLKRIMSINENVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYDGIKDYPVCFYDPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSIDEMTNALDPVELAEIINKKHHAIINGKKFKTSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRERIGETGTVLGLNARDSKHAGRMFSMHNCQINPGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
BASE COUNT        14062 a        16434 c        15094 g        13961 t
ORIGIN      
        1 ccctttggcc ccgttctatt tattagtgaa attacaatct caccggatcg atatgccaga
       61 tatgatcagc gtactctttg atagtacggt cagaagagaa gtagcccata ttggcaatgt
      121 tcagcatcgc ttttgcggtc cactcttcct gaagctcgta gagttcgtcg actttatcct
      181 gacaatcgac atagctgcga taatccgcca gtacctggta gtgatcgccg aagttgatca
      241 gcgaatcaac cagatcgcga tagcgacccg gatcttccgg actgaataca ccgctgccga
      301 tttgcgtcag cacctgatgc agctcctcat ctttctcgta gtattcacgc ggtttgtagc
      361 cctgacgacg cagttcttcc acttcttccg ctgtgttacc aaaaataaag atattgtcag
      421 caccgacatg atccagcatc tcgacattcg caccgtccaa cgtaccgata gtcagcgcac
      481 cgttaagcgc aaacttcatg ttactggtgc cggaagcttc cgtccctgcc agcgaaatct
      541 gttcagacag atctgccgcc ggaatgatca actgcgccag gctaacgctg tagttcggga
      601 tgaacacgac tttcagctta tcgccaatct gcggatcgtt gttgatcact ttcgctacgt
      661 cattgatcaa atgaataatg tgcttcgcca tgtaataggc cgaagccgcc ttaccgccaa
      721 aaatattcac gcgcggtacc cacttcgcat ccgggtcggc cttgatgcgg ttatagcggg
      781 taatcacatg caacacattc atcaattgac gtttgtattc gtgaatacgt ttgatttgta
      841 catcgaacaa cgcctttgga ttcaccacca cattcagctg ctgggcgata tactctgcca
      901 gacgcttttt gttctccagc ttcgcctgat gcacagcgtg attaaccatt gggaaatcac
      961 agtgttgttg cagctcatta agcaggctaa ggtcggtgcg ccagttacgg cccaggtgtt
     1021 cgtccagcac ggctgaaagc gatgggttcg ctaccgccag ccagcgacgc ggcgtcacac
     1081 cgttggtgac gttggtgaaa cgacccggga agattttcgc aaagtcggca aacaacgatt
     1141 gcaccatcag attagagtgc agttccgata caccgttaac tttgtggctc acaacaaccg
     1201 ccagccaggc catacgcaca cgacgaccgt tggattcatc aatgatcgac gcccgtccca
     1261 gcagatcggt atcgttcgga tactgttcct gcaaggtttt caggaaatag tcgttgattt
     1321 caaagatgat ctgcaggtga cgcggcagaa ttttacccag catatcaacc ggccaggttt
     1381 ccagcgcctc gctcatcagc gtgtggttag tgtaggagaa gacctgacaa cacacctcaa
     1441 acgcgtcgtc ccagctaaat tggtgctcat cgatcagcag acgcatcatc tcaggaatcg
     1501 acagtaccgg atgggtatca ttgagatgaa tcgcgatttt atccgccagg ttatcgtagg
     1561 ttttatgcaa ctgataatga cggcttaaaa tgtcctgaat ggtcgaggaa accaagaagt
     1621 attcctgacg caggcgcagc tcacgcccgg agtaggtgga gtcatccgga tacagtacgc
     1681 gagatacgtt ctcggagtgg tttttatctt ccactgccgc gaagtagtca ccctggttga
     1741 atttaccgag gttaatttcg ctactggctt gcgcactcca caaacgcagc gtgttggtcg
     1801 cgtcggtgtc gtaaccaggg attatctgat cgtaagcgac tcccagaatc tcttcggttt
     1861 caatccagcg cgttttttta ccttcctgct gaatgcgacc accaaaacgg actttatagc
     1921 gcgtgttgtg gcgtttgaat tcccacgggt taccgtattc cagccagtag tctggcgact
     1981 ctttctggct accgttaacg atgttctgct tgaacatacc gtagtcatag cggatgccgt
     2041 aaccgcgccc cggcaaccct aacgtcgcca gagaatcaag gaagcaagcc gccagacgtc
     2101 ccaggccacc gttaccgagg cctgggtcat tttcttcatc tatcagctct tcgagattta
     2161 accccatcgc ttccagtgcg ccctgtacat cttcgtaaat tcctagcgac aacatggcgt
     2221 tggagagcgt acggccaatc aaaaactcca tcgacaggta gtaaacctga cgagtttctt
     2281 gcgacaactg ggcacggttt gaacgtaacc agcgctccac gagacgatcg cgcacagcaa
     2341 ataacgttgc gttcagccat tcatgtttat tggcgacgac cgggtccttt ccaatcgtaa
     2401 acatcagctt gtaagcgata gagtgcttaa gagcttctac gctaagcgtg ggcgatgaat
     2461 atgtaaacgg agcattcata taggcgtttc ctgaaaacta tttcaagcga tagtaaagct
     2521 cacggtacga cttcgccgcg acctgccagc taaaatccat tgccatagcc tgacgttgca
     2581 caaaccgcca cagtgaagga cgggaccaca gtacaaaagc acgccgaata gcccgtaaca
     2641 gcgaccaggc attactatct tcaaagacaa acccactggc gacgccatct gcaaggttct
     2701 caagagaaca gtcagaaacc gtatcagcaa gcccaccggt gcgccgcact aacggcagcg
     2761 taccgtactt caatccataa agttgcgtta agccgcacgg ttcaaaacgg ctgggcacca
     2821 gaatgacgtc cgcgccgccc ataatgcgat gcgaaaatgc ttcgtgatag ccaatctgaa
     2881 cgcccacctg accggggtat tccgctgccg ccgcaaggaa accttcctgc agcaccggat
     2941 cgcccgcgcc gagtagcgcc agctgcccgc cctgctccag aagccccggt aaggcttcca
     3001 gcaccaggtc gagacctttc tggctggtca gacggctcac cactgcaaaa agcggcactt
     3061 tatcgtcaac cttaagcccc attgcgattt gtaactggct cttattttcc gctttatctt
     3121 ccaacgtatc gcgggtgtaa cgcgaggcca acagtaagtc cgtctctgga ctccagattt
     3181 tctcgtccac gccgttcagt acgcctgaaa gacgcccttc acggtgacgc tgttgcaaca
     3241 gaccttccat accgtaggca aactgcggtt cggtgatctc gcgagcgtag gttggactga
     3301 ccgccgtaat gtgatcggca tagtacagac cggccttcag gaaagagatt tgtccgttga
     3361 attccagccc atgaatatta aagaatgacc atggcaattg gatgtcattc atgtgatgtg
     3421 cataaaacat gccttgatag gccaggttgt gcacagtaaa caccgacttc gcaggacgcc
     3481 cgcgcgccgc cagatacgca ggcgcaagac ctgcatgcca gtcgtgcgca tgcaccacat
     3541 caggacgcca gaatgggtca agcccgctgg ccatttctgc cccaacccac cccagcagcg
     3601 caaaacgcaa tacgttgtcg gtataggcaa ataagttggt atcgtgatac gggcttcccg
     3661 gacgatcata gagatgcggc gcgtcaatca ggtatatgcc aaccccgttg taatgaccga
     3721 acaacagcgt gatatgcccg gcgaaagtat cacgacggga tactacctgc gcatcggtca
     3781 cgccacggcg aatatcggga aatgcaggca acagtacgcg agcgtcaacg ccgtctgcga
     3841 tttgtgctgc gggtaatgcc ccaataacat cagccagacc gccggtttta agcagcggga
     3901 acatctctga acatacatgt aaaacctgca ttatcgctcc tgtttatgcc ctaacttccg
     3961 tagcatttcg cgcgttacca gcacgatgcc ttcttctgaa cgatagaaac gacgtgcatc
     4021 ttcctctgcg ttttcaccaa tcaccatgcc ttccgggata acacaagcac gatcgatgac
     4081 gcagcggcgc agacggcacg agcgacctac ccatacttcc ggtaacaata cggcggaatc
     4141 aatgttgcag aatgaattca cgcgaacgcg cgagaacaga acggactgca ccaccaccga
     4201 accggagatc acacaaccgc cggataccag tgagttaagg gtcatcccgt ggctaccgga
     4261 gcgatcctgc acgaatttcg ctggcggtaa tgattcattg taggtgcgaa ttggccaatt
     4321 gcgatcgtac atatccagtt ccggcaccac agaggccaga tcgagattcg ctttccagta
     4381 agcttccagc gtacccacat cgcgccagta cggctcggca tccgggtcgg attgtacgca
     4441 agagagcggg aacgggtgcg cataggccag accggcttcg gtgatcttgg gaatcaaatc
     4501 tttgccaaag tcgtggctgg agttctcatc acgatcgtct tcttccagca gttcatacag
     4561 atagtcggcg tcaaagacgt agatacccat actcgccaga gatttgctcg gatcgttcgg
     4621 cattgacggt gggttagcag gtttttcaac gaattcgata attttatcgt tctcatcaac
     4681 cgccataacg ccaaatgcgg aggcttcttc aatcggtact ggcatacaag caacggtgca
     4741 acgtgcgcct ttttcgacgt gatcgataag catacgcgag tagtcttgct tgtagatatg
     4801 gtcgcccgcc aggatcacca cgtattccgc tttataacgg cggataatgt cgaggttttg
     4861 ggtgaccgca tctgcggtgc cgcgatacca gttttcccct ttcattctct gctgtgctgg
     4921 cagcagatcg acaaactcgt tcatttcttc attgaagaat gaccagccgc gctgaatgtg
     4981 ctgcaccaga gtgtgggact ggtactgggt gatcacgccc atacgacgga tcccagagtt
     5041 gatgcagtta gacagcgcaa agtcaataat gcggaactta ccgccgaagt gtacggccgg
     5101 ttttgctcgc ttattggtta aatccttcag gcgggtacca cgtcctcccg ccagtatcag
     5161 ggcaacagat ttcaatggca gctggcgcgc caacattaag tgatcgttct tctctaaact
     5221 aaccatgact aactcctttt ttatcatctc tggaacacac acaatccgtg tgcaggtccc
     5281 tgccagacaa ccgtaatcac tgggttatcc tctccagcga atgggggaat ggcgtgccac
     5341 tccccagcag gtaaaacaat ctctgttacc tcaagcgtgg cgttaattgc gatcaaaaag
     5401 cgatccgaga gcagaatttg cagctgtttc ggcccgtttt gccactcatc cgtgcttaaa
     5461 ggttgagcat atcgatttag ccaacggaca ttgccgtcac cttcttccca ccagcgattc
     5521 tccaccaaag cgggaatgcg cttgcgcaga tggattaacg cggcggtaaa tgcggttaaa
     5581 ccactgcttg cctgcgacca gtccaaccag tttaattggt tatcctgaca gtaggcattg
     5641 ttattgccat gctggctgtg accatgttcg tcaccggcca gtaacatcgg cgtgccctgg
     5701 gagagcaaca acgttgttaa cagggcatga atgctgtcgc gccgccgttc aaccaggtca
     5761 agagtaccgc ctaacccttc tttaccatga ttgttactgt aattgttgtt ggtcccgtcg
     5821 cgattttctt ctccgtttgc ttcattgtgt ttatggttga agcaaacgca gtcgcgaagc
     5881 gtaaaaccgt catgcgcggt gacgagatta atcgcggcac tcggcagacg accattacgt
     5941 ttaaaaacat cgctggaggc agcaaaacgc ccggcaaacg cccccagagg caaatcataa
     6001 tgtagccaga aacgacgggc agcatcgcgg aaatgatcgt tccactcggc aaacagcggc
     6061 gggaaatttc ccacctgata accactagga gcgatatccc acggttcagc aattaacttc
     6121 acctgcgaga gcaccgggca gttctggata gcggtaaaca acggcgcatc ctgacggaac
     6181 tctggcgtac ggcccatgac tgccgccaga tcaaagcgga aaccatcgac gtggcaggtt
     6241 tctacccaat aacgcaggca ggcgctggca taatccacca ccgccggatg actcaaattg
     6301 agcgtgttgc cgcaaccggt ccagttgtga taatcgccgt cttctcttat ccaataatag
     6361 ctacggttat cgatcccacg cagcgagaat aacgggccgt cgaggtccag ttccgcacta
     6421 tggttgagca cgatatcaag aatgacttcg atacccgctt tatgcagtgc tttgattgca
     6481 tcgcgaaact catccagcgc cgtttctggc gagcaggcat acgccggatg cagcgcaaac
     6541 atcgccaccg ggttgtaacc ccagtagtta cttagcccca tgcgttgcag acgtggttca
     6601 ctggcaaact gcgccactgg cagcagttcc agcgcggtaa tgcccaattg tttcaaatag
     6661 ttgatcatca ccggatgccc gagggcttta taagtgccac ggatctcgac cgggatctcc
     6721 gggtgcaagt acgttaatcc tttgacatgg gcttcataaa tgatggtgct gccccacggc
     6781 gtgcgcggcg gggcgtcatc ttcccagtca tagtgatcaa ccaccactac gcatttcggc
     6841 gcaatggcgg cgttgtcgcg atagtcaggt tcattatgac cggcgtgcag cagcgggtta
     6901 tctttaaact ccccgtcaat ttgccgcgcg caaggatcaa tcaacaactt cgccgggtta
     6961 aagcgatgcc cctcggcggg ttgccagggg ccatgaacgc gataaccata acgcaaaccc
     7021 gggcgcgcat ccggcagata accgtgccaa atgtcgccac tgttccctgg caagtcatag
     7081 cgatgttcct ggccattggc gtcaaagaca cacagttcta cccgctcggc atgagcggag
     7141 aaaagtgtga agttgacgcc ctgaccgtcg taatgcgcgc cgaggggagc gggtttgcca
     7201 atggcgagtt gtgtcattct gcctcccgaa ccagccagat agtggccagc ggtggtagcg
     7261 tcaggcttag tgaatgctga cgaccgtggc tggcaatctc atcgctgtgt accgtgccgc
     7321 cattgcctgc attactgccg tgatagtgta tggaatcggt attgaggatt tcacgccatt
     7381 tacctggctg attaatgcag aagcgataat catgacgcgg caccggcgta aagttactgg
     7441 caacgataat ttcgttaccc tctttatcgc gacgcacaaa gatcagcacc gagcgttctt
     7501 tgtcatccac caccagccat tcaaagccgt acgggtcaaa atccagttca tgcattgctt
     7561 tatggtggcg gtaggtgagg ttcagatcgc gcaccagacg ctggacaccg tggtgccagt
     7621 tatcgccgcc ttccaacaga tgccagtcga ggctggcgtc atggttccac tcgcggccct
     7681 gggcaaattc gttccccatg aacagcagtt tcttgcccgg aaatgcccac atccagccat
     7741 agtaagcgcg caggttagcg aatttctgcc atgcgtcgcc cggcatgcgg tcgagaatcg
     7801 attttttacc gtggaccact tcatcatgcg acaacggcag gacgaagttt tcagtgtagt
     7861 tgtagagcat cccgaaggtc agtttatcgt gatgatactg acgataaatc gggtcgagct
     7921 tcatgtagtc cagggtgtca tgcatccagc cgaggttcca cttgtaccag aagcccagac
     7981 cgcccatatc ctgcggacga gaaacgccag ggaaatcggt agactcctca gccattgtca
     8041 ccgcaccgga aacctgctca ccaagaatac ggttggtatt acgcaagaat tcaatcgctt
     8101 caagattctc gcgcccgcca aattcgttcg ggatccactc cccctcttta cggctgtagt
     8161 cacgataaat catcgacgcc accgcatcga cgcgcagcgc atcaatacca aaacgttcaa
     8221 tccagtaaag cgcgttaccg acgaggaagt tactgacttc acggcgacca tagttgtaga
     8281 tcagcgtgtt ccagtcctga tgatagcctt cacgcggatc gctgtgttca tacaagttcg
     8341 tgccatcaaa ttcggcaagc gcaaagtcat cagtcgggaa gtggcctggc acccagtcga
     8401 gaatcacgtt cagaccagct gcgtgtgcgg catcaatgaa ataacggaag tcgtcgcgag
     8461 taccaaaacg gcgggttggt gcatacaggc cggttggctg ataaccccaa ctgccatcga
     8521 agggatgctc gttaatgggc agtagttcga ggtgggtaaa gcccatccat ttagcataag
     8581 gcaccagttg atcggccagc tcgcggtagc tcaaccagaa attgttgtcg gtgtggcgac
     8641 gccaggaacc caggtgaact tcataaatag agattggcgc atcaaactga ttcgcttttt
     8701 tgcgctcttc agtctgtaca accttttccg gcagcccgca aataagagac gcggtttccg
     8761 ggcgcatttg cgcttcgaag gcataagggt cggacttcag acgcaagttg ccattggcat
     8821 caatcatctc gtatttatag agctgaccgt tatgcgcccc agggataaac agttcccaga
     8881 tgccgctctc tttacgcagg cgcatcgggt gacggcgacc gtcccagtag ttgaattgcc
     8941 caaccaccga gacccgacgg gcgtttggag cccagacaga gaaacgcgta cctgtgacgc
     9001 catccatagt atctgcatgc gcgcctaagg tttcatacgg gcgcaggtga gtaccttcag
     9061 ataatagcca ggcatccatt tcctggatta gcggaccaaa acggtaagga tcatcaatca
     9121 ggttttgctg accatgccag acaacagcca actgatagcg gaaaaaattc ttacgtcgcg
     9181 gaatgacgcc gctaaagaat ccccgtgagt cgagacactc cagttttgcg agtttgcgcc
     9241 cggttttcgg ttcaatcacc cacacatcgg tagcgtcggg taaaagggca cggacttcca
     9301 gtcccgcggt ggttttatgc attcccagta cggaaaaagg atccgcaaaa tggcctgcaa
     9361 ttagcgcgtt aatcacgtct ctatcgatac gatcggacat gcttgtcttc ctgttttatt
     9421 gtgtcacccc atccaattgg atttttgact tctggttgtg acattttttt gacctgaacg
     9481 gcgcagcact ctgtgcatcc tctctgcgtc gtcctcactt caggtaaggc tgtgaatact
     9541 catgtattca gccaccctta aagaatagcc aatgctctat ttaactcccg gtaaatcatg
     9601 aaacatctgc gcttactcct gtattacgca ctaacagggg cggcatcgcg ccccagattt
     9661 aatgaataaa gattacgcca gttgacgaag catccgacgc agcggctccg cggcccccca
     9721 cagcagctgg tcgcccacgg taaaggctga caggaactct ggtcccatat tcagcttacg
     9781 caggcggcct accggcgtgg tcagcgtgcc ggtaacggca gctggggtta gctcacgcat
     9841 agtgatttcc cgatcgttcg gaacgacttt cgcccacgga ttgtgcgcag ccagcagttc
     9901 ttcgacggtc ggaatagaca catctttttt caatttaata gtgaatgcct ggctgtggca
     9961 gcgcaatgcc ccgacacgca cacataaacc atctaccgga attacggaag atgtgttgag
    10021 gatcttgttg gtttccgcct gccctttcca ctcttcgcgg ctctgaccgt tatcgagctg
    10081 tttgtcgatc cacggaatca ggctacccgc cagcggcacg ccaaagttat ccaccggcag
    10141 ctcaccgcta cgggttaagg ttgtgacttt gcgttcgata tcgagaatag cagaggacgg
    10201 ggtcgcgagt tcatctgcca catggccata cagatggccc atctgggtta ataactcacg
    10261 catatgtcgc gcaccaccgc cggaagcggc ctggtaggtt gcaacggaca cccaatcaac
    10321 aagatcattg gcgaataaac cacccaacga catcaacatc aggcttacgg tacagttacc
    10381 gccaacaaaa gtcctgatgc cattatttaa tccgtcggta atgacgtcct gattgacggg
    10441 gtcaagaatg atgatggcgt catctttcat gcgcagagac gatgctgcgt caatccagta
    10501 accttgccat ccgctttcac gaagctttgg atagatttcg ttggtataat cgccgccctg
    10561 acaggtcaca atgatatcga gggcctttag cgcctccaga tcaaaggcat cctgaagtgt
    10621 gccagtggtt ccgccaaaag acggcgcagc ctggccaagc tgagaagtag aaaagaagac
    10681 agggcgaatg gcgtcgaagt cgcgctcttc aaccatgcgt tgcatgagaa cggagccgac
    10741 cataccgcgc cagccgataa aaccaacatt tttcataagc gttttttcct gcaaagatgt
    10801 gtgctgtata aatgtgccgg tctcctcttg gcacatcttt caccatacaa aaagcagcca
    10861 aagtcgcaag tgaaattaat caatgatagc gaagccatca gtaatgcgac ttatcctgct
    10921 ttgttagcac gcagaaagtc cgcggcaatt atcagggaat ttgagttatg aatgaaatca
    10981 tttctgcagc agttttattg atcctgatta tggatccgct cggaaacctg cctattttca
    11041 tgtccgtact gaaacatact gaaccgaaaa gacggcgggc aatcatggtg cgagagttgc
    11101 ttattgctct cctggtgatg ctggtgttcc tgtttgcggg tgagaaaatt ctggcatttc
    11161 tcagcctgcg agcagaaacc gtctccattt ctggcggcat cattctgttt ctaatcgcca
    11221 ttaagatgat tttccccagc gcttcaggaa atagcagcgg gcttccggca ggtgaagagc
    11281 catttatcgt gccgttggca attccgttag tcgccgggcc gactattctc gccacgctga
    11341 tgttgttgtc tcatcagtac ccgaatcaga tgggacatct ggtgattgct ctgctgctgg
    11401 cctggggcgg cacctttgtc atcctgctac agtcttcgct atttttacgt ctgctgggcg
    11461 agaaaggggt gaacgcactt gaacgcctga tgggattgat tctggtgatg atggcaactc
    11521 agatgttcct cgacggcatt cgaatgtgga tgaaggggta acgaggagag tttttcgaaa
    11581 agagcccggt agcgtcacaa ctaccgggcg aacaaactta actcaacagc tggaacgcaa
    11641 tcatcccaac gatggcacca acagtgccga ggatggtttc catcatggtc caggttttca
    11701 gcgtttcggc ttcggtcgcg ccggtaaatt taccgaacaa ccagaaacca gcgtcgttaa
    11761 cgtggctgac aacaatcgaa ccaccagcga tacaaatcga cagtgccgcc atttgcgcac
    11821 cagagtagtt cagttgttca atgactggca tcaccagtcc caccgccgtt aaacaggcta
    11881 cggtggcagc cccctgaatg atgcgcactg cagcagccag cacgaagcag gtgatagcaa
    11941 tcggcaggcc catgccggtt aacgcttcgc ccagtgccgg acctacgcca gagtcaacca
    12001 gcacctgttt gaacacgccg cctgcaccaa tcaccagcag aatgatcccc gccggttgca
    12061 gcgcgtgacc gcaaatctcc atcactttgt cttttggcat gccctgacgc attgccaggc
    12121 cataaatcgc taccagacaa gcaaccagaa tcgcggtaaa cggatgacca ataaactcga
    12181 accattcgta agcggtagag ccttctggca caaaacgcgc ggcaatggtt ttcagcccca
    12241 ccagcaccag cggcaacagg atcagcgaca ggctgaatcc gaaagatggc attttacctt
    12301 cgccgagatg cggttcgctg atgtcgtcag gaatatgcag ctcaacgtaa cggctgatga
    12361 agttacccca cagcggcccg gcaataatca ttcccggaat tgccgcacac aggccaatca
    12421 ggatcatcca gccaaagtcg gcattcatct gcgatgccag cagcattggc gctggcccag
    12481 gcaccaggaa cgctgccgcg gccgccacgc ctgcgaataa tgggattacc agcttcacca
    12541 ggttcgtacc ggtgtggcgc gccattgaga aagcaacgct aatcagcaga acaatcgcca
    12601 cttcaaagaa cagcggtagc gcacagacca gccccgcaag gccgatggca taatgcgcgc
    12661 ggctgtgacc gaaggatttg agcattttga cggcaatctg atcgactgcg ccggtttcat
    12721 ataagatctt gccaaacata gctcccaggg cgacaaccac cgccaggaag ccgagggtgc
    12781 ctcccatccc tttttccatc gtcgctgcga ttttatcgag cggcataccg gaaaaaaggc
    12841 cagcccccat ggacaccacc atcaaagcca ggaaagcgtg catacgcgcc ttcatgacta
    12901 aaaacagcag cagtaaaaca gaccctactg ctgttaaaac aagcgttaat gtagtcacta
    12961 cttatttgcc ttttttaata acctcaatgg tgcttgccac aacaccttcc agcggttgat
    13021 cgatatccac caccagtaca tcggtttcgt ccgcacccgg ctcctgcagc gtttcaaact
    13081 gcgtcaccaa catttgggtt ttaaagaaat ggcctttgcg cgctttcagg cgactttcaa
    13141 tcacatcaaa atcgcctttc aggtagatga aagagagatt cggattacct tcacgcagca
    13201 agtcgcgata gtgctttttc agtgcagaac aggcgatcag cgacacttta ttggtgcgct
    13261 gcatagcaaa cgcggagtcg ttcagcgcct gcaaccaggg tttgcgatcg tcgtcattca
    13321 gcggttcgcc agacgccatt ttttcgatat tgcgccgtgg atggaggaaa tcgccatcaa
    13381 gaaacgcggc atgaagttga tgcgccactt cactggcgac cgcagatttg ccgctgcccg
    13441 atacgcccat caagacgtaa atgtggtgat catggttagt cgtgctcaaa gtggtgcccc
    13501 cacaatacaa gaattaaaaa tgttacgggt aactgttatc ggcaacattg tccagccgga
    13561 caatatcaga agcaatatcc atccgtgcct taagtgtata agtgtgagct acttcaaatt
    13621 tgtgggctta aatagatccg cccggtgaca aggtgaaacc taaatctaac attttcggtg
    13681 tcacagattc gccacgaata cgcgccagca ggcgttcagc gccaatactg cccatccgct
    13741 cacgcggcgt cagcacgctc gcaagtcgtg gctccatcac ctgaccaatg tcatgaccgt
    13801 ggaaaccggc aatcgccata tcgtcaggaa cttttaaccc cagacgctga cattcaaacg
    13861 ccgcgccgac cgccagatca tcattagtac agaacacgcc atccagctgc ggatattccc
    13921 gccgcgcctg gcgaatcagt tcaataccgg aagagtaaga agaagattgc tcaaccatca
    13981 cgctatatgg caccaggcct gcatccagca tcgcctgttc gtatcccttc tgtttgatga
    14041 tagtacgttc gtcgagacgt gcgccgagat aggcaatgtg gcgatgccca cgagcaataa
    14101 tggcggtagt catctggcgt gccgcttcaa agttatcaaa accgacggcg atatcgaggc
    14161 acggcgactg gctgtccatc aactccacca ccgggatccc cgccacttca atcatcttta
    14221 aggtgcgcgg cgtgtgggta cgttcggtga ggatcaggcc gtcgatattc caggagagca
    14281 tggattcgag acgttcttgc tccatttccg gtttataacc gtagtgcgcc agcatggtct
    14341 gataaccgtg cgcgtcggtg acgctttcga ttccgcgtaa tacttccgcg aaaacctggt
    14401 tggtgagaga aggtaacagg acgccaatcg cccggctggt agcgttagag aggatatcgg
    14461 gcgcacgatt gggaatatag cccagttcat caagagcggc ggcaatcttg ccgcgtagag
    14521 cgacggaaac ctgctccggg ttgcgtaaaa aacggctgac cgtcattttg gtcacgccta
    14581 cacggtcagc cacatcctga agtacgggtc ttttcttttt catcgtcctg aaggtacaaa
    14641 agagatagat tctcttagtt taacacggac gcatcagaac cttcccgggg aaaacagtgc
    14701 ttgtttacaa ttattagata tacactaaat caattgcatc gcattgtgct aacgacgcgg
    14761 caaaaccccg gaagcgtgca tcagcacgcg accggagcga gcgaggaagc ctgcaaaaag
    14821 gcaggcttca aagatgccgt tgagttttaa accggcggca gatcgaacag taacacttcg
    14881 ctgtcgctat ccgcatggat ggagattgcc tgctcatccc agattgccag accatcgctg
    14941 gtcgaggctt tcacgccgtt aatggtaaca ttgcctttta ccacctgaat ccagacgcgg
    15001 cgttcagcgg caatctgatg caccgactgc tcatctttca gcaacgccca acgatacagt
    15061 tccatatcct gatgcacttt cagcgaacca tctcgcgcat ccggcgagag caccagctgt
    15121 ttgccctgca cggcatcgaa gcgacgctgt tcataacgcg gcgtaatgcc attttcttca
    15181 ggcatgatcc agatctgata cagatgcaga cgctcggtgc tgcttgggtt gtactctgag
    15241 tgacgaatac ccgtacccgc actcataatc tggaactcac ccgccggaac ctgctcttta
    15301 ttgcccatgc tgtcctgatg ctcaacagta ccttccagca cgtaggtcaa aatttccata
    15361 tctttatgcg ggtgagtgcc gaagccctgc cctgcttcaa tcacgtcgtc gttaatcacg
    15421 cgcagcgcgg agaagcccat aaagttcgga tcgtaatagt tggcacaaga gaaagtatgc
    15481 caggagtcca gccagccatg atttgcatga ccacgttcat ttgctttgcg taagtagatc
    15541 atttgtattc gccccctgaa tgatttcgat gggcttagtg tggacccgat ctgcctggga
    15601 tgatagaggg tgaaaattga cccctctgtt caaaaaaatt gaactattcg aggggccatc
    15661 aaacttaatt cgcgagagtc actgtggagg gagaggcttg ctcaaatccg cgttcaagga
    15721 tttccagatt ggtaagaact tcagattcct tgacgtaatt tggcgcaccg tgggtgatgg
    15781 tttgatacaa cgcatcataa acacgcccgt aatcgcccat ctccggcttc atctcttctc
    15841 tgaccgtcac accctcgtca ttgacatact ccagcacacc gaccgaatca tccgctgcga
    15901 atcccggttc gcccggcata atattagcct tcaggctggt ttcctgctgg tcgataccgt
    15961 atttaataaa cgaacctttc ttaccgtgaa cgataaattt cggataatcg attttcacca
    16021 gatggctggt tttgacgatg gctttcaggt cgccataaaa cagttgcgct tcaaaggtgt
    16081 cgtcaggatt ggctttatta cgcaggctgc ggatgtcata agcgacgtga tccgggcgac
    16141 cgaacagaga aataatctgg tccatcgtat gcacaccaag gccatagaac gcgccatcct
    16201 gcggcagtcc aggtttggtt tctgccaccg ggcggtaata gtcaaaatgg ctttccactt
    16261 cgacaatctc tcccagcttg ccgctttcaa tcgctttttt cgccgtcagg aagcaggaat
    16321 caaagcgacg attctgatat ggcgtgacgg tcagcccttt gcttttcgcc agtgcaaaca
    16381 gctctttcgc ctgcgcaagt gtcggagtga acgttttttc gaccagcaca tttttcccgg
    16441 cttccagcgc gcgcttcgcg tactcgaagt ggctgtccgc atgggtgcag acaacaacca
    16501 acttaacatc gggatcgttt agcacttcgt cgagatcgct ggtgaaatgg atatgagagt
    16561 aaatgggagc ctgttcttcc ggcttcgcat ggcgacgaaa aatatgcgcg acatgccagc
    16621 tatccttgcg gttaagtaca tacggcagat ggtaacgggt ggtgcttttg ccgaagccaa
    16681 taaaggcgca gttgatgacc atgatttcgt cctttttaag gtggttattt acaccttagc
    16741 gcaaagcgga cgtggttcct actggagtgc gcataacgaa cacaagcact cccgtggata
    16801 aattgagaac gaaagatcaa aaaaaaagcc agcacccggc tggctaagta atactggaag
    16861 caatgtgagc aatgtcgtgc tttcaggttc tccgcgaggg tcttcctgat cgcgacacaa
    16921 taataatcat tctcattcgc acttgtccaa cactttttgc aaaaaaatgc atttgactcg
    16981 catttgaaag tcaatgatgt tgaaagggac atttacccca aagaggacaa aggaatgagt
    17041 gagatagtaa tacgccacgc agaaacacgg gattacgagg ccatcaggca gattcacgcc
    17101 cagccggagg tgtattgcaa cacactacag gtgcctcatc cttccgatca tatgtggcag
    17161 gagcgactcg ccgatcgtcc cggcatcaag caactcgtcg cctgtattga tggagacgtc
    17221 gtgggccatc tcaccattga cgtgcaacag cgcccacgcc gcagtcatgt tgccgatttt
    17281 ggtatctgtg tcgactctcg ttggaagaac cgcggcgtcg ccagcgccct gatgcgagag
    17341 atgattgaaa tgtgcgacaa ctggttacgg gtagatcgca ttgaactaac cgtgtttgtc
    17401 gataacgcgc cagcaattaa ggtctataaa aaattcggct ttgagattga agggaccggt
    17461 aagaagtacg cattgcgtaa tggtgaatat gtcgatgcgt attatatggc gcgggtgaaa
    17521 tagagaattt gcaatctaag tttgtcattt aagccctcac ctaccgggag ggcttaagat
    17581 attaataccc cgccgttaaa tcatccaccg agcgcgggtc ggatgcgccg tacaactcac
    17641 cgtccggccc aaccataatg ctttgtgtgc tgcccatcgc ctctttcagc gccactttct
    17701 gaccttttgc ttccagcagc ttgagcgtat ccgggctaaa ccctttttcg acacgcagct
    17761 catccggcaa ccactgatgg tggaaacgcg gcgcattggt cgcttcggcg acgttcatgc
    17821 cataatcgat gctattcacc accatttgca gcaccgtggt gataatccgg ctaccgcctg
    17881 ggctaccggt aaccagccag gttttaccgt ctttcaccac aatggtcggc gacatcgacg
    17941 acagcgggcg tttgttcggc ccgacggcgt tggcatcacc gcccaccagc ccgtaaacgt
    18001 tcggtacgcc cggtttggcg gagaaatcat ccatctggtt attaagcaga ataccgctct
    18061 cacccgcgac aatacccgta ccgaaggtgg tgttcagcgt ataggtcacc gccaccgcgt
    18121 taccgtcttt atccactaca gagtaatggg tagtttgatt actctcataa ggcgcaagct
    18181 tgccagggcg aatctcactg gacggcttcg cttttttgat atcaatttga tcggcaatgg
    18241 atttggcata ggctttattg gtcagtgcct gccacgggac tttgacaaaa tccgggtcgc
    18301 caagatattc cgagcggtcg gcgtaggcgt atttctccgc ttctgccatg atttgcatcg
    18361 catcggcgct gccaaagccg tatttcttca tatcgaagtt ttccagaata ttgaggattt
    18421 gtacgatatg gatcccgccg gaggatggcg gtggcatgga gtaaacctga tacccgcgat
    18481 aatcgccgct tatcggagtg cgttcgaccg ctttataggc cgctaaatct tctttagtga
    18541 tcaagccacc gtttttctgc atctcctggg cgatctgttc cgcaatcgtg cctttataga
    18601 attcgtccgg gccgttttca gcaatcatct ccaggctctt tgccaggttc gcctgcacca
    18661 gcgtgtcgcc ctttttcagc ggctcgccct ctttccagaa gatagcttta ctgttttcgt
    18721 gattcggcaa cacttcgcta ccgtaggttt tgagatcgtc agccagcgcg tcgttaacga
    18781 taaaaccatc gcgtgccagt ttaaacgcgg gctgcacgac tttgttcagc ggcatggtgc
    18841 cgtatttatc cagcgccagc gagaaacctg ctaccgtacc cggtgtgccg gaagccagat
    18901 gcgaagtgag tgattttttg ctgtccgggt tgccctgatc atcgaggaac atatcgcggg
    18961 tcgctttggc gggtgccatt tcgcggaaat cgatagccgt ggtattgcca tttttcgagc
    19021 ggattaacat aaaaccacca ccgcccagat tccctgcctg cggatgcgtt accgccagcg
    19081 cgtagcccac cgccacggcg gcatcaacgg cattcccgcc ctccttgagg atatccaccc
    19141 ccacctgagt ggcagtggcg tccacagacg ctaccattcc ctgtttcgcg cgtaccggat
    19201 ggaagacatc ttcctccaca ccatacgaga cgggcggcgc aggaggcgcg gcggcggcgc
    19261 taaaacaact tcctgagagc agagcagcaa tggccacccg gcgtaaaaac gtcggtttta
    19321 tcatcgttat tctccagaga ttaaggggca accccagcta agcctggtat ataactctga
    19381 attaatcatc gttttgtcgg gaagcgagta aacttaaagg atatcttcag aggaggatac
    19441 gcgatgaaac gacttctgat tcttacggca ctcctgccgt ttgtcggctt tgcacagccc
    19501 attaatactc tgaacaaccc taaccagccg gggtatcaga tacccagcca gcagcggatg
    19561 caaacccaga tgcagactca gcaaatccag caaaaaggga tgctgaatca gcaactgaaa
    19621 acgcaaactc agttgcaaca gcagcattta gaaaaccaga taaacaataa ttctcagcgg
    19681 gtgttgcagt cacagccggg ggagcgaaat cccgcccggc agcaaatgct gcccaacacc
    19741 aacggcggga tgttaaacag caaccgtaat ccggatagtt cgttgaatca gcagcatatg
    19801 ctgccggaga ggagaaacgg cgacatgctg aatcagccca gcacgccgca gcctgacatt
    19861 ccgttgaaaa ctattgggcc gtaaagttcg gaccaatcac gtcaatcgca tcggtacaga
    19921 tgcaatccac gccccagcgc agcaactctg ccgcgtgctg gggtttattg acggtataaa
    19981 ccagaatccg taatccggcg tctttcaact gcatcactcg cgctttatcg agtaacttat
    20041 gattgagatg aatagagacg cagcccagcc gcgcggtcag ttcgcgccag tcgtcgcgcc
    20101 actcatcaag caataaacca cgcggcagtt ccggtgccgc ctgttgtgca gcttctaaag
    20161 catcaatctc aaacgatgac agcagcggcg gcgtcatacc ggcccacagt tggcgtgccg
    20221 ccagcgccac cattttgccc gttaatggtc cagtgccagt ggtgggtttg atttcgatat
    20281 tcgccatcat cccgtgttcg cgacagcgtt ccgccacctg cgaaagcaac ggcagcggct
    20341 cacctttaaa cgctttgctg taccaactgc ccgcatccac gcgcagtaaa tcctgccagt
    20401 tcagttcacc cgcgacgccc cagccgttgc tggtgcgttc gagattgtcg tcatggagca
    20461 ggaagatctc gccatctttc gataacttcg cgtcaaattc gatcatctta tgaccgtatt
    20521 ttgccccgac gtcgattgcc gccagggtgt tttccggggc cagcttaccg ccgccacgat
    20581 gagcgacgat gcggggataa ggccagttac tcatactcgt tgtcctgttt caccatcaaa
    20641 aagatgcagc tgattttccg ccagatgcag ccacagcgtg ctgcctgccg tcgggcgctc
    20701 ctgatgcgcc agtcgcacca ccagcttctg cttgcgagct tagcgcaata tgttccgggc
    20761 gaatgccgag ggtcatttta cgcccggcat actgacggta gccaccgttt agcggtagca
    20821 cgataccgcc gtccagttcg aaatgcgtgc cttcattatt catgcggcct gtcagcaggt
    20881 tcatcgccgg gctgccgata aaactcgcca caaacaggct ggcgggcttt tcgtagactt
    20941 ccaccggcgt gccaatctgt tcggcaacgc cgccgttcat caccattact cgctgggcga
    21001 gcgtcatcgc ctcaacctga tcgtgagtaa cgtagagtga agtcgttttc aggcgacggt
    21061 gcagctgttg cagttcaaga cgcatctgta cgcgcagctt ggcatcgagg ttagagagtg
    21121 gctcatcaaa caagaacacc gccggatcgc gcacaatcgc gcgtcccatc gccacacgct
    21181 ggcgctgacc gccggaaagc tcgcgcgggc gacgtttgag cagaccgtcc agctccagaa
    21241 tgcgcgccgc ttctttaacg cgctcggcta tttgctgctt gcccatgccg cgaattttca
    21301 gcccccacgc catgttttct tcgacgctca tatgcggata aagcgcgtaa ttctggaaca
    21361 ccatcgcaat cccgcgatct ttcggctcca tttcggtcac gcgctggtcg ttgatccaga
    21421 tatcgccttc ggtcaccctc tccagcccgg caaccatacg cagcagcgtc gatttcccgc
    21481 agccagacgg cccgaccatc acgataaatt cgccatccgc cacatcaagg gtcagcggtt
    21541 taatgaccta ggttttgcca tcccagcttt tggttactgc ctgtaatttc agtcctgcca
    21601 tcttatttct cactatcgac caggccgcgc acgaaggcac gctgcatcac taaaacaatc
    21661 actactggtg ggatcagggt taacaacatc gccgccatca ctgagttcca ttccgtggtg
    21721 ccttcgccag tggcgatcat ccctttgatc cctgccacgg tggtgccgag atccacgtcg
    21781 gtaataatca acaacggcca caaatactgg ttccagccgt aaataaaggt gatcacaaac
    21841 agcgccgcca gattagtttt ggagagcgga aaaacgatgt cgcaaaagaa gcgcattggt
    21901 gacgcaccgt cgatccgcgc cgcttccacc agttcatccg gcagcgtcat aaagaactgg
    21961 cgaaacagaa acgtggcggt cgccgaggcc atcagcggca gcgttaaacc ggcgtagctg
    22021 tcgagcatct tcaggttggc gatgacttcc accgtcggga agatacgtac ttcaaccggc
    22081 agcatcaggg tgataaaaat catccagaag aagaggttac gtagcggaaa acgaaaccag
    22141 acaatggcaa atgccgagag catcgagaca gtaattttgc cgagcgtaat gctgaacgcc
    22201 atcacaaagc tgttaagcaa catccgccag aacggcgcgc tatttgtgcc taccccgttc
    22261 acccagatgt tgtggatgtt ttccagcaga tgcgtgccgg ggatgagcgt catcggcgcg
    22321 gcgtagacgg cctgtttatc cagcgtcgcc gcgacaaacg ccacgtacag cgggaagagg
    22381 atcaccgcga tcccgaggat cagcatggta tggctgaata tcgtcagcca cggacggttc
    22441 tcaatcattg gtaacgcacc ttgctttcaa catagcggaa ctgcaccacc gtcagcacga
    22501 tgacgaggaa catcaacacc atcgactgtg cggcagacga agccagatcc agtccggtaa
    22561 aaccttcgcg gtagatctta taaatcagcg tcgtggtggc ctgaaccggc ccgccggacg
    22621 tggcggcgtc gatcaccggg aaggtgtcga agaaggcata caccaggttc actaccagca
    22681 ggaagaaact caccggggcg ataagcggca gcgcaatctt aaagaagcgg cgaatcggcc
    22741 ctgcaccgtc gatggctgcg gcttcgatca acgaacgggg aatggattgc agcgcggcat
    22801 agaagaacag aaagttgtag ctgatttgct tccacaccga ggcaaacacc accagaaaca
    22861 tcgcctgacc gctattttgc gcgtggttcc agtcgtagcc gaactcagcg agaaaatggg
    22921 tgatcagccc gcgaccgggg ttaaacagga agatccacaa tacggcggca acggcgggag
    22981 ccacggcata cggcagcaac attaaggttt ggtagaaacg actgccgcgc acgatgtact
    23041 ccaccagcgc cgcaaagaac agcgatacca gcaaaccgct gacggtgaca aaggtgctga
    23101 attttatcgt cgtccagaag gagtcgagat aatagctgtc atgaaacagc atgacgaagt
    23161 tatccagacc gacaaactgg ctggagaaac caaacggatc gacgctttgt agcgagtacc
    23221 acaacgcttc gcccgcaggc cagataaaaa agataacggt gatgatgagc tgcggcgcga
    23281 ccagcagata aggcagccag cgcgagcgga acaccggacg ggatgatgac attacggtta
    23341 attcctgaac tgtgccggat gcgcttcgct tatccggcct acacgcgatg ccaggtcaga
    23401 taagcgaaac gcatccggca ttacactgat taagacttcg tcgatttctc aaagcggcgc
    23461 agtaactggt tcccacgctc aactgcggta tccagcgcct gctgtggcgt cttcttaccg
    23521 gtccacacgc tctcaagctc ttcatccaca atcacgcgga tctgcggcat gttgcccaga
    23581 cgaagccctt tggtgaacgg caacggcggc ttgttcagca tctgacgcgt cgcggtatcc
    23641 gcacctgggt ttttctcgta aaagccctgc tcacgggtca ggtcatacgc cgctttggta
    23701 attggcagat aaccggtttt ctgatgccac tcagcagcgt tttctggctt cgcgaggaag
    23761 tcgagaaact tcgccacgcc ggtatacgtt tctttatctt tgccctgcat cacccacagg
    23821 ctggctccgc cgataatggc gttttgtggc gcatctttcg catcggcgtc gtaaggcatc
    23881 atacctacgc cgtagttaaa tttggcgtac tcgcgaatgt tggcaagaga accggaagag
    23941 gcggtggtca tcgcgcaatc accgttatag aacttctcgg tggattcatc cttacgaccg
    24001 acgtagctga agtcgccctt cttgttcatc tcctcgagca tggcgatgtg tttcacctgc
    24061 tccggcttgt tgaactccag caccgcgtcc gtgccgtcaa agccgttgtt tttgctggca
    24121 aacggcagac cgttccaggc gctaaagttt tccagttgga tccagccctg ccagccgctg
    24181 gcgtagccgc acttaatacc ggaggctttc agttttgcgg aatagtccgc cagatcctgc
    24241 caggttttcg gcggctgttc cgggtctaac cccgctttct tgaaggcgtc tttgttgtaa
    24301 tagagaacgg gagtcgagct gttgaacggc tgggagagta agtggccggt tttgctgtcg
    24361 gagtagtaac cggaaaccgt cggcacaaac tgcgactcat cgaactgaat ccctgcctct
    24421 ttaaacacgt catacaccgg tttaatggct ttcgacgcca tcatggtggc ggtgccaact
    24481 tcataaacct gcaaaatagc cggagcgttg ccggtacgaa atacggcaat ccccgcgctt
    24541 aaattctgtt cgtagttgcc tttataggtc ggtacaattt tgtaatccgg attttcggcg
    24601 ttaaaacgtt gggctagaga atccacctct ttacccagtt ccccttccat agaatgccag
    24661 aacggaatgg tcgtcactgc ctgtgcattc cccattaacg ccagtccgag cgccagtgct
    24721 gaagctgtat aatgtaacgg tttcatcgtt tatctctctt gttgtaccga atgcgcgaat
    24781 tcacgcgttt tatgctcgcg gggtaacatg acatgctcga attacagaaa aataactttt
    24841 ttgttacatt tgtaagatag taaggtgtca gaaagatgac aaggcggtga cggcgtgggt
    24901 gagggaaaat gggagatggg gcacggataa gcggaaaaat agaaggtctg aatcaaactc
    24961 tacagattac tcatcgtttc atgccggatg cggcgtaaac gccttatccg gcctacaaga
    25021 tcgtgcaaat tcaatatatt gcaactcacc cagtaggcct gataagcgca gcgcatcagg
    25081 caattttgca tttgtcacct gtctcaaagg agtcttttga ctccttatca atcaacgtgt
    25141 tattacccgc ctaaatacgc acttctcact gcttcgttcg ccagcagcgc gtcgccggta
    25201 tcggagagca ccacatggcc gttttccagc acgtagccgc gatccgccag ctttagcgcc
    25261 tggttggcgt tctgctcgac gaggaagata gtcatcccct gctcgcgcag ctgctcgatg
    25321 gtgtcgaaaa tttgctggat gataatcggc gcaagaccga gcgatggctc atcaagtaac
    25381 aacaaacgcg gattgctcat cagcgcgcga ccaatcgcca gcatctgctg ttcaccgccg
    25441 gacatggtgc ccgcccgctg aacacggcgc tcatgcagac gtggaaacag ttcatacacc
    25501 cactttatgc gctcctgaaa ctggtcacgt tcggcaaaga agccgcccat cgccaggttc
    25561 tcttccaccg tcatccgcga gaagacgcga cgcccttccg ggacaatcgc taccgcttcg
    25621 cgcatgattt tcgccgtctg ccagtcggta atgtctttat catcaaacac aattcgcccg
    25681 ctggtggcgc gcggatcgcc gcataacgtg ccgagcaagg tggttttccc cgccccgttc
    25741 gcgccaatga gagtgacaat ctcgccctga ttgatatgca ggctcacctc atgcagcgcc
    25801 tggattttgc cgtagtgggc gctgactttg tcaaaggaca acatgacttt ttccatctta
    25861 tgcctcacct aaataggcac ggatcacgtc cgggttatta cggatttgct ccggcgtacc
    25921 gtttgccagt ggcgtcccct gattgaccac ataaattcgg tctgaaatcc ccatcaccag
    25981 cttcatatcg tgttcaatca acaagatagt ggtgttgtga tgattgcgca gttcggcaat
    26041 cagctcatcc agctctttgg tttcttttgg gttaagacct gccgccggtt cgtcgagcat
    26101 taaaatctcc ggctgcgtca ccatgcagcg ggcaatctcc agacggcgtt ggtcaccata
    26161 ggccaggtta ctcgcctgac ggttggcgtg ttccagcaaa ccaatgcgct caagccaggt
    26221 ggcggcgcgg tcgagcgctt cgctctgggc gcgacggaaa gaaggcgttt tcaacaggcc
    26281 agagaacagc ccggttttca gttgctgatg ctgcgccacc agcaggtttt caattaccgt
    26341 catttcacgg aacagacgca catgctggaa ggtgcgcacc acgcccatgc gagcaatttg
    26401 ctgccccggt aaaccttcaa gatgctgatc gcgcagtaaa atggtgccgc cggtgggttt
    26461 gtagaaaccg gtcaggcagt taaaaaccgt ggtttttccg gcaccgttag ggccgattaa
    26521 cgagacgatt tcctgcgggt acagttcaag attgacgttg ttcaccgcca gcaggccgcc
    26581 gaagcgcatc atcagaccgt taacagataa taatggctga ctcatgcctg ctctcctttc
    26641 gctgcgccgt ttttcagctt cagttgcggg cgcgtcatgg gcagcaaacc ctgcggacgc
    26701 cagatcatca tcagcaccat caaaccaccg agcattaaca tgctgtattc gttgaaatca
    26761 cgcatcaatt cgcgcgacac caccagcaga acagccgcca gaatcactgc aaattgcgag
    26821 cccataccgc cgagcaccac tatcgccagc acaaaggccg attcagcaaa ggtgaaggat
    26881 tccgggctga caaagccctg acgcgccgca aacagcgttc cggcaaaacc ggcaaatgcg
    26941 gcgctgatgg taaaagcagt cagcttgata cggcgcgggc ttaagcccag cgaacgacag
    27001 gcgatctcgt cttcacgcaa cgcttcccac gcgcggccca gcggcatccg cagcaggcgg
    27061 ttaatgacaa acaggcttaa caccaccagc agcaacgcca ccaggtagag gaagataacg
    27121 cgatcggacg gatcgtattt caggccaaag aaattactga acgtgtccca gccgccttca
    27181 cgagcggtac ggctgaactc gagtccgaag agtgtcggtt tcgggatctg actgattccg
    27241 ttcgggccgc cggtgatttc agtgttattg agcagcaata tgcgcacaat ttcgccgaaa
    27301 ccaagggtaa cgatcgccag atagtcaccg cgcagacgca gcaccggaaa accgagcagg
    27361 aaacccgccg ccgctgccat cagtccggca atcggcaaac aggtccagaa ccccagaccg
    27421 taatagtgat tgagcagcgc aaaagtgtaa gcgccgatgg cgtaaaaacc gccgtacccc
    27481 agcaccagca gaccagaaag accaacaacc acattcagcc cgagaccgag gataatgtag
    27541 atcatggtca gagtggcgat atccaccgtc ccacgtgaca ccataaacgg ccacgccacc
    27601 acaagcacca gcagcgccac gaggaacagc ttttgcttca ccgtggaccc atcaatcgcg
    27661 ggcagaataa acttcggccc ggaaacgctt ttcaaccctt tctggaaagc cggtcgcaaa
    27721 agctggaaga aaaagaccac cgccgtgccg ataaacatcc actgccaacg gatgtctgaa
    27781 gccgtgtcga ccaccagttt ggtgccatcc agctccagtt gtacgcccat aaagacgccc
    27841 gccaggacaa agaacatcgc ggcagagagc agtgccattg caatatgcat cggtttcata
    27901 ctttctctac ctccgggcga cccagaatac cggtcggcat caccagcagc accagaatca
    27961 gcagggcgaa tgagaccaca tctttatatt ccgtactcag ataggcagaa gagagcgcct
    28021 ccgcaatccc caggatcagc ccgccaatca tcgcccctgg aatgctgcca atcccaccga
    28081 gcaccgcagc ggtaaaggct ttcatcccgg ccataaagcc gatatagggg ttaatgacgc
    28141 agtagaactg accgagcagt acgccagcca ctgccgccat cgccgcgcca ataacaaagg
    28201 tcaaagcaat cacccggtcg gtgttgatgc caagcaggct cgccattttc agatcttccg
    28261 cgcaggcacg acaggcgcgc cccatacggg aatagcgaat gaaaatcgtc agcgccagca
    28321 tggcgaggaa ggtgacaatc cagatcaccg cctgcatggt ggtaatagag gcagagaagt
    28381 tttcgctatg gcccaccacc cactgaccgt taaacaggct cggcagcgcc acgtcgcgcg
    28441 aaccttcggt caggctgacg tagttttgca ggaagatgga cataccgatt gcggaaataa
    28501 gcgcaatcag gcgcttagag ttacgcaccg ggcggtaagc tacccgttcg atactccagc
    28561 cgtaggcgct ggcgatgacg attgcgccga cgaatcccgc cgccaccagc agccagccgg
    28621 tatcaatgcc catcatcatc agcgcggcga tgatcataaa tgagacgtag ctgccaatca
    28681 tataaacctc gccgtgggcg aagttgatca tgccgataat gccgtaaacc atggtgtagc
    28741 cgatggctat cagcgcgtag gtactgccca gcgtgacgcc gttaaacatc tgctgcaaga
    28801 aatacaaaaa ctgctcagac ataaggtaac ctttctaaac ccgcccgcat tttacgggcg
    28861 gtgggatgat cacttggctg ccgtggatga accgtcggcg tgccactgga agacaccaaa
    28921 atcaaatccc ttaagatcgc ctttttcatc ccagttcagc ggcccaatca cggtgtttgc
    28981 accgttagct tttaaatctt tcaccagcgc cagcggctca tcgctgccgg tacgctcaag
    29041 ggcagttgcc agagattgca ctgccgcgta agtgatccag acataaggcc cggacggatc
    29101 tttcttgtct gctttcagcg catcaacgat gccctggttt gccggatcct ggtcatagcg
    29161 ttttggcata gtgaccaaca tgccttcggc ggcatcaccg gcaatgttcg acagcgatgc
    29221 gttaccgaca ccttccggcc ccataaactg ggttttcagg ccaacggaac gggcttggcg
    29281 cagcatctgc cccatttccg ggtagtaacc gccgtagtaa acgaagtcga tattttcttt
    29341 tttcaggcgg gcgatcagcg cggagaaatc tttctccccg gcggtaatac cgtcgaagaa
    29401 gacgacgttg gcgttagccg ctttcagccc gtcctgcacc gaacgcgcca gcccttcgcc
    29461 atactgttgt ttgtcgtgaa tgatggcgat gcgctggggc ttcaccgtct caagaatgta
    29521 ttttgccgcc gtaggcccct gggaagagtc cagcccggca gtacgcataa tgtgttgata
    29581 accgcgttgg gtcagctccg ggttggtcgc tcccggcgag atcatcagaa taccttcgtc
    29641 ttcgtagata tccgacgctg gctgggtaga ggaagaacac agatgaccaa taacgtattt
    29701 aatgccgtcg ttaacgattt tgttggcgac cgcaacggct tgtttcgggt cgcaggcgtc
    29761 gtcatattcc acgccaacca gcttgtcgcc cttaattccc cctttgacat taatgtcttt
    29821 aatcgcctga cgcgcgccgt taaattccat atcgccccac tgggcaatcg ggccggacat
    29881 cgcgccgaca acggcgactt taatatcgtc agccatagcg gtgtgtgaaa ttgccagtgc
    29941 aatcatccct gcgatgatag ttttcgcatt ccgtttcata gtcaaaaatc cccattcgtg
    30001 atgttgtgtt gctttgtttt tatgtgttaa caaatcagac tgttcttttt ttatactgca
    30061 ctgtttttgc ctgtctgatt aaggggttag cgcagtattt tgtgatgata gcgattaaaa
    30121 tccctatttt tcagtcgatt aagaacagat aatattctga atttattgat agataaacag
    30181 aaaaaagtgc ctttgtcagc ataaaataac ggcacaaagg gcggaataat tcactatcat
    30241 tcaggggatt atgctggaca tttttcattc tctaatgttt taactttgta attattgctg
    30301 ttaaaaaatt aatcacctgc caaaagaaat aaaaaagaga aagcctccga ttaaattatt
    30361 tcgctacact ggttccactt ttgtgattta cacgggttac ccatgaagct gaccatcatt
    30421 cgattagaaa actttagcga ccaggaccgg attgacctgc aaaagatctg gccggagtat
    30481 tccccttcct ctttacaggt tgacgataac caccgtatct acgccgcgcg ttttaacgag
    30541 cgtctgcttg cggccgtgcg ggtaacctta agcggcaccg agggagcact ggattctcta
    30601 cgcgtgcgag aagtcacccg ccgtcgcggt gtggggcaat atctgctgga agaggttttg
    30661 cgtaacaatc ctggcgtttc atgctggtgg atggcggatg caggcgtgga agatcgcggt
    30721 gtgatgacgg cgtttatgca ggcactggga tttacggcac agcagggcgg ctgggagaag
    30781 cgttaatcgt caagttgatt tcaaaagtga tgaccaacga gaaattgcct gatgcgctac
    30841 gcttatcagg cctataatgt gtgctgcaat ttactgattt ctttggttct tgtaggccgg
    30901 ataaggcgtt tacgccgcat ccggcatgaa gcaacgtatt cgatattagc aatttggcgg
    30961 caacccaaag ttgccgatta acaattactt cgcatcggtc gccgtgccgt tggcgtgcca
    31021 gtcaaatacg ccgaactcaa agccctttag atcgcctttc tcatcccagg tcagtggccc
    31081 catcacggta tccacggagt tcgcttttag gtatttggcg atttcagccg gatcgtcaga
    31141 ctgattcagg cccgcctgca aagattgcag cgcggcgtag gtggtccaga cgaatgcgcc
    31201 gcttgggtcc tgttttttcg ctttgatcgc atcaacaatg ggtttgttcg ccggaacctg
    31261 atcgtagttc ttcggcttgg tcaccagcag ctcttccgct gattcgcccg caatgttaga
    31321 cagcgaaacg ttagccacac cttccggccc cataaactga gttttcagcc ctgccgcgcg
    31381 tgcctgacgc aggatttgcc ccatttccgg gtgataaccg ccgtagtaaa cgaagtcgat
    31441 attctctttt ttcagacgcg ccaccagcgt tgagaaatct ttttccccgg cggtgatacc
    31501 atcaaagaac accacgttag cattgccttt cttcaggccg tcctgcaccg ctcgtgccag
    31561 accttcgccg tattgctgtt tgtcgtgaac gatagcaata cgctgcggtt tcactttctc
    31621 aagaatatat ttcgccgccg tcggcccctg gtcggagtcc aggccggtgg tgcgcaggat
    31681 cagctgatag ccacgggcgg tcagctccgg cgcggttgcc gctggggtga tcattaaaat
    31741 gccttcgtct tcgtagatgt cagacgcagg ctgcgttgat gaagaacaga ggtgaccaat
    31801 cacatattta atgccgtcgt taacgacttt gttcgccacc gcaaccgcct gtttcgggtc
    31861 acaggcatcg tcatatttta cgatttgcag tttgttgcct ttaatgccgc ctttagcgtt
    31921 gatatccgca accgcctgct ctgcgccggt aaactcctgg tcaccgtact gcgcaaccgg
    31981 accggacatt gcacccacga ccgcgacttt aatatcttct gccagagcca tattgctgaa
    32041 tgccagcgcg atacatcctg ccagtaacgc tttacccttt atgttcatcc tgagaatccc
    32101 cattcttctg gttattacgt gtgttgtgat gttgttgttc agcactttat ttcgttttat
    32161 gcatgactac ccgtgcttta gcagcatact ctgctaaaac atacccgatt tttatgatat
    32221 tggaatagct attttgacag tttattaaca atctgcgtgg ggattggcgt tttgccggag
    32281 ggggaattga ttattgtaga ggcccaaaaa acaaaaaccc cggactctca tccagggttc
    32341 tctgcttaat agcggaaatt acgcttcaat agcggcgcga agttttttca tcgcgttctt
    32401 ttccagttgg cgcacacgtt cagcggaaac accgtaacgg tcagccagtt cctgcaacgt
    32461 ggacttgttg tcttcgtcca gccagcgcgc acggatgatg tcctggctgc gttcgtccag
    32521 accatgcatc gcgtcggtca gacggtttgc cgcctgctct tcccagttat catcttcgat
    32581 gccgtcggca aagttagatg atttatcctg cagatagagc accggtgcca tcggctggct
    32641 gtcggaatcg tcgtcggaag acaggtcaaa ggtcatgtcc tgtgccgcca tacgtgattc
    32701 catctcacgt acgtctttgc tggttacgcc cagttcacgg gccaccattt cgacttcatc
    32761 ctggttaaac cagcccagac gctgcttggt tttacgcagg ttgaagaaca gtttgcgctg
    32821 cgctttggtg gtcgcaactt tgacgatacg ccagttacgc agaacgtatt cgtggatctc
    32881 tgctttgatc cagtggacgg cgaaggagac caggcgcaca cccacttccg ggttaaaacg
    32941 gcgcactgct ttcatcaggc cgatgttacc ttcctgaatc aaatccgcct gtggcaggcc
    33001 atagcccgca taattacgag caatatgaac aacaaaccgc aggtgagaca ggatcagcgt
    33061 tttagctgct tccagatcgc catggtaatg cagcttttca gccagcgccc gctcctcgtc
    33121 agccgacaac atcggccacg cgttagctgc ccggatgtag gaatccaggt tgccaactgg
    33181 ggctaaagct aaactttgca ttttgtcagt cattcaaatc ctctcaatcg atatcttctg
    33241 gcgcttcagt ggtagcaaca actgtgccaa agcttaagag caacgaggtt atcattcact
    33301 gttttatcag accgtgattt tatccacaag ttcaatgcaa gcttgtgaat aaattacgca
    33361 caaaatgtga catagagatg aaataccggg aagagacaac ggggtctctt tccctgctac
    33421 ggaacccatt gcagggaaag agtataacac gcttttatta ttcaggcgta aagtggcgta
    33481 aatgttgtac cgtggcaagc cacgctgcca cccagccaat catcgagcat accagcagca
    33541 atagcaggca ttcatcgaac gataaaccat tgatatcaaa cttcgttccg aaaacctgtg
    33601 ccacttccgc caccgctgac gacaaacgca gtaccagaat ttctgacaaa attaatgaca
    33661 acaatgcgcc agaaaatccc agcagcgcac cgccgtacag gaacgggcga aggatgaaac
    33721 catccgtcgc accaatcagt ttctgcacgt tgatggagtc acggcgagca aagatgctca
    33781 gacgtacgct gttgccgatg acgaggaaca ctgccgccac catcaacacg ccgatcatcg
    33841 ccgaaacgcg cccaaccagc ccggtcaacg ccgccagacg ggcaaaccag ctgtcatcca
    33901 tccgcacttc gtcaataccg ttaatctgcg tgatacgatc acgcagcgta ttgagtgatt
    33961 ccgtcccctg aaaatcgagt ttcgggatca ccaccgccac tgccggaagc gggttttctt
    34021 ccagcatatc cagcgcacca ccaaaaccag accagttacg gaactcgccc agtgcgtctt
    34081 cacgagaaag atagttcact ttctccacgc cttgctcggc ctgcaactgt gccaccacgc
    34141 ccgcagcggc gtcatcgtcc agcgtttttt gcaagtaaac agtgatttgc ggtgacggat
    34201 aatactgcgt cgccgcctga ttaacgtttt tgtacaccat ataacagacg ctgggcagcg
    34261 tcagggagat ggcgataacc atcaccgtta aaaacgtggc gaacggtttg cttttcagat
    34321 cctgcaatgc gccgtggaag gcatagcgca cctgttcgtt gaaaacgttg gttttgcgat
    34381 ttaccggttt tggcgaggat ttcgcgcgtt tcggtgcgtt acgaccgccg tcgcctgagc
    34441 cgccgaccga tttacggaag cgatccagac gcccaccaaa ctgccgaata tgattgattg
    34501 catcgcgctt attcatggcc cacgcctcca tgcaagtgac catcgctcag ggtgagcatg
    34561 cgataggaac gccgtgaaat caggttaatg tcgtgcgttg ccatcaatac ggttacccca
    34621 acgcggttaa actcttcaaa cagacgtaga atcccttccg acaacgcgtc gtccaggtta
    34681 ccggtcggtt cgtccgccag cagtaccgcg ggcttattca ccaccgcgcg ggcaatgcca
    34741 acacgctgtt gttcaccgcc ggaaagctga atagggaagt tcttcgcttt gtccagtagc
    34801 ccgactttat ccagtgccgc cgacacccgg cgacgaatat cgtcaccgct ggcaccagcg
    34861 ataatcagcg ggatcgccac gttatcgtag acagtacggt ccatcagtag atggtgatcc
    34921 tggaaaatca tgccaatctg gcggcgcaga aacggaactt cacggttttt cagacgcgtg
    34981 atgtcatggc cgctaaacca gattttcccg gcgctgggcc gctcaatccc acagatcagc
    35041 ttcaggaggg tacttttccc tgcgccggaa tgaccggtca gaaacgccat ctcacccggc
    35101 tgcatatgga acgtaacgcc ctgcagcgcc tgtctcccac cgagataagc cttgctgaca
    35161 tgttcaaagc gaatcattgt taatcctctc gggcaaaaag tgcctctata aagtcgtccg
    35221 ccttaaacgg acgcaaatcc tcaatacgtt cgccgacacc aatgtagcgg atagggatac
    35281 caaactggtc agccaccgag aaaattaccc cgcctttcgc cgtgccgtcc agtttcgtta
    35341 gcgtgatgcc ggttaagcca acagcttcgt ggaacagttt ggcctggctt accgcgttct
    35401 gcccggtgct ggcatcaata gtcagcataa cttcatgcgg cgcttcaacg tcgagtttct
    35461 tcatcacgcg gacgattttc ttcaactctt ccatcagatg cgatttgttc tgcagacgtc
    35521 cggcggtatc ggcaatcagg acgtcgatat tacgcgcttt agctgcctga atggcgtcga
    35581 agataacaga ggcggaatcc gcaccggtat gctgggcaat caccggaata ttgttgcgct
    35641 gaccccagac ctgaagctgt tcaaccgcag ctgcacggaa agtatcgccc gccgccagca
    35701 tcaccgattt accctgctgc tcaaactgac gcgccagctt accaatcgtc gtggttttac
    35761 ccacaccgtt gacgcccacc atcaggatca cgaacggcgt tttatcttca acattcagcg
    35821 gctcatcgac tttcgccaga atctcgccca tctcttcttt cagcaggcca tagagcgcct
    35881 cggcatcacg aagctgcttg cgggatgcgc cttccgtcag attggtgata attttacgtg
    35941 tggtttccac gcccacatcg gcgatcaaca gctgttcttc cagctcttca aatagatcat
    36001 cgtcgatttt tttaccgcgg aacaggctga taaatccgga accgagattt tctttggttt
    36061 ttaacaggct gcgtttcagg cgcgcgaaga agccttcttt ggtcggtttt tcttgctcct
    36121 gggcaatttc tgcaaccggc tgctcttctt ctgccggagg aactaccatc accgcctctt
    36181 ctgccgcttc ggcagccagc gcctgtgctt ccagctcttc gtcggtgatt tcttctttag
    36241 ccgcttcttc ttctgccgct tcgacaatct ctacggtttc cgcttcagcc tgccactctt
    36301 ctggcgaaac ctcttcggcg ttgacgtctt ccggcaacgg cagctcttca cgttcgatag
    36361 ccactggctc cggcgtttct tctacgaccg gttccggctg tgcaacaact tccgcttcag
    36421 gctgcgcttt ttcactttca acaacctgtt cagtgacttc cacaacgtcg gcagcaaaag
    36481 tttccgcctc ggcttcagta tgcgcctgcg gctgctcttc aacggcttgt tcagaagcct
    36541 tcgcaggctc ttgcgcctga acgatttctt ctacaaccgg ttgttcattc tgaacttctg
    36601 tctctttttc cggggtctgc tctttttgac caaagcccag ccaggaaaaa aagccacgtc
    36661 ttttttcttt cgccatttgc gactacactc ctcgctgttg cttcatggca cagcgttaac
    36721 gctatgtaca tagcagctaa aaaaatgatg aaatagtcta tcacttaact taattcacat
    36781 caccgcctgc aagtatgtgt tatctggcgg attgagcaat ttatcatgaa aaaaccgaat
    36841 cattccggca gcggccaaat ccgcattatt ggcgggcagt ggcgaggccg taaactcccg
    36901 gttcctgata gcccaggtct gcgccccacc accgaccgcg tacgcgaaac gttgtttaac
    36961 tggctggctc cggtcattgt tgacgcccaa tgtctggatt gcttcgccgg gagcggcgcg
    37021 ctggggctgg aagcgttatc gcgctacgct gcgggggcaa cgttgattga gatggatcgc
    37081 gtggtttctc agcagttaat taagaatctg gcgacactaa aagcaggcaa tgcacgcgtg
    37141 gtgaacagca acgcaatgtc attcctggcg caaaaaggca caccgcataa tatcgtgttt
    37201 gtcgatccac cgttccgccg tggcttgtta gaagagacga taaatttact ggaagataac
    37261 ggctggctgg ctgacgaagc cctgatttat gtcgaaagcg aagtcgaaaa cggtctgccc
    37321 actgttccgg caaactggtc attacatcgg gaaaaagtgg cgggtcaggt ggcttatcgg
    37381 ctgtatcaac acgaagcaca aggagaaagt gatgctgatt aatattggtc gtttgttaat
    37441 gctctgcgtt tggggatttt taatcctcaa tctggtgcat cccttcccgc gcccgctaaa
    37501 tattttcgtt aacgtggcgc tgatttttac cgtgctgatg catggtatgc agctggcgct
    37561 attgaaatcc actttaccga aagatggccc gcagatgacc accgccgaaa aggtgcggat
    37621 tttccttttc ggcgtgtttg aactgctggt ctggcagaag aaatttaaag ttaaaaaata
    37681 accttattgt tcgccaacaa agctaacaaa gcgcgttcct ttatagctca gcgtaccttt
    37741 atcgcccact gtcagggcgt ggtactgctg ggcgtcgagg cgaaacgtct gctccattcc
    37801 tccgctttgc ggtttgaagc ttgcctcata gcgcatactg gtgcctgccg gagtcacttc
    37861 ctgctggcgc gaacggcgat cgttaatcgg tttttcccgc ttgttgctca ccaccaccag
    37921 cttttgctgg agcggagcca tatcattatc agctttttcc cgtcgctgtt gcataaaacg
    37981 aaacgatgcg gcgacgacaa ttaagccaat gataacaata aagaaaagag gtggtttact
    38041 catctttatc cctcatcgga aaatgcggaa ttaagcatac cctgccagtt atggtgttgt
    38101 catccgtcca ccctcgccac taaactggaa gcaagaccgt aggcattctg cttacgaaaa
    38161 aataacgaat tcaaggaact aagatgcttt ggtcgtttat cgctgtctgt ctttccgcat
    38221 ggctatctgt ggatgcatcg tatcgtgggc caacctggca acgctgggtg tttaaaccgt
    38281 taacccttct tctcctgctg ttactggcct ggcaagcacc gatgttcgac gccattagct
    38341 atctggtgct ggcgggactg tgcgcctcac tgctgggcga tgcgctaacc ctgttgccac
    38401 gtcaacgtct gatgtacgcc atcggcgcgt ttttcctctc gcacctgctg tacaccatct
    38461 atttcgccag tcagatgacg ctctctttct tctggcctct gccactggtg ttgctggttc
    38521 tgggtgcgct gctactggcg attatctgga cgcggctgga agagtaccgt tggcctatct
    38581 gcacgtttat cggcatgacg ctggtgatgg tgtggctggc aggtgaactg tggttcttcc
    38641 gtccgaccgc tccggcgctc tctgcgtttg ttggcgcttc gttgctgttt atcagtaact
    38701 ttgtctggct ggggagccac tatcgccgac gcttccgtgc ggataacgcg attgctgcgg
    38761 cctgctactt tgccggtcac ttcctcatcg tccgctcact gtatctctga taaaacttga
    38821 ctctggagtc gactccagag tgtatccttc ggttaatgag aaaaaactta accggaggat
    38881 gccatgtcga cacctgacaa tcacggcaag aaagcccctc aatttgctgc gttcaaaccg
    38941 ctaaccacgg tacagaacgc caacgactgt tgctgcgacg gcgcatgctc cagctcgcca
    39001 acgctctctg aaaacgtctc cggcacccgc tatagctgga aagtcagcgg catggactgc
    39061 gccgcctgtg cgcgcaaggt agaaaatgcc gtgcgccagc ttgcaggcgt gaatcaggtg
    39121 caggtgttgt tcgccaccga aaaactggtg gttgatgccg acaatgacat ccgtgcccag
    39181 gttgaatctg cggtgcaaaa agcgggctat tccctgcgcg atgagcaggc cgccgacgag
    39241 ccacaagcat cgcgcctgaa agagaatctg ccgctgatta cgcttatcgt gatgatggca
    39301 atcagctggg gtctggagca gttcaatcat ccgttcgggc aactggcgtt tatcgcgacc
    39361 acgctagttg ggctgtaccc gattgctcgt caggcattac ggctgatcaa atccggcagc
    39421 tacttcgcca ttgaaacctt aatgagcgta gccgctattg gtgcactgtt tattggcgca
    39481 acggctgaag ctgcgatggt gttgctgctg tttttgattg gtgaacgact ggaaggctgg
    39541 gccgccagcc gcgcgcgtca gggcgttagc gcgttaatgg cgctgaaacc agaaaccgcc
    39601 acgcgcctgc gtaacggtga gcgggaagag gtggcgatta acagcctgcg gcctggcgat
    39661 gtgattgaag tcgccgcagg tgggcgtttg cctgccgacg gtaaactgct ctcaccgttt
    39721 gccagttttg atgaaagcgc cctaaccgga gaatccattc cggtggagcg cgcgacgggc
    39781 gataaagttc ctgcaggagc caccagcgta gaccgtctgg taacgctgga agtgctgtca
    39841 gaaccgggtg ccagcgccat tgaccggatt ctgaaactga tcgaagaagc cgaagagcgc
    39901 cgcgccccca ttgagcggtt tatcgaccgt ttcagccgta tttatacgcc agcgattatg
    39961 gccgtcgccc tgctggtaac gctagtgccg ccgctgctgt ttgccgccag ctggcaggag
    40021 tggatttata aaggtctgac gctgctgctg attggttgcc cgtgtgcgtt agttatctca
    40081 acgcctgcgg cgataacctc cgggctggca gcagcagcgc gtcgtggggc gttaattaaa
    40141 ggcggtgcgg cgctggaaca actaggtcgt gttacccaag tggcgtttga taaaaccggt
    40201 acgctgaccg tcggtaaacc gcgcgttacc gcgattcatc cggcaacggg tattagtgaa
    40261 tctgaactgc tgacactggc ggcggcggtc gagcaaggcg cgacgcatcc actggcgcaa
    40321 gccatcgtac gcgaagcaca ggttgctgaa ctcgccattc ccaccgccga atcacagcgg
    40381 gcactggtcg ggtctggcat tgaagcgcag gttaatggtg agcgcgtgtt gatatgcgct
    40441 gccgggaaac atcccgctga tgcatttgct ggtttgatta atgaactgga aagcgccggg
    40501 caaacggttg tgctggtagt acgtaatgat gacgtgctgg gtatcattgc attgcaggat
    40561 accctgcgcg ccgacgctac aactgccatc agtgaactga acgcgctggg ggttaaagga
    40621 gtgatcctca ccggcgataa tccacgcgca gcggcggcaa ttgccgggga gctggggctg
    40681 gagtttaaag cgggcctatt gccggaagat aaagtcaaag cggtgaccaa gctgaatcaa
    40741 catgcgcccc tggcgatggt cggtgatggt attaacgacg cgccagcgat gaaagctgcc
    40801 gccatcggga ttgctatggg tagcggcaca gacgtggcgc tggaaaccgc cgacgcagca
    40861 ttaacccata accacctgcg cggcctggtg caaatgattg aactggcacg cgccactcac
    40921 gccaatatcc gccagaacat caccattgcg ctgggactga aagggatctt cctcgtcacc
    40981 acgcttttag ggatgaccgg gttgtggctg gcagtgctgg cagatacggg tgcgacggtg
    41041 ctggtgacgg cgaatgcgtt aaggttgttg cgcaggagat aaggcaaacc gatcgcaaca
    41101 ttgagcgcga tcggtcccct cgcccctctg gggagagggt tagggggtga ggaaaaggcg
    41161 gcatcgaagc caatcagccc ctatcaaccg cctttacgaa tcaaataacg ataaggcagt
    41221 ccatccgtct ctttcgcaac cagttcgtgt tccataaagg tacaaaaccc aggaatatcg
    41281 cgggtagtgg ccggatcgtc ggcgataatc agcaacgttt cgccaggctg catattgcgc
    41341 acggttttgc gcaccatcat caccggttcc gggcagcgca ggccaagcgc gtcgagtgtg
    41401 tggtcagggc tggaaaagag atcggtcatt ttcttctcat cacttaaaaa aacggcgcta
    41461 gtttacgccc tgtgagtccg taagcaaacc aggttaacga ttgcgtgaaa attaaccatt
    41521 gcattgtcaa cgtaaagcag tatcatgcgg cggctcgaaa aaagggtaag cacgttatta
    41581 tgttaaggta acagacgtgt cgtacgtatt gggttccctc accccaatgg ttaatcaaaa
    41641 aggtacaata tgaacgtttt ctcgcaaact caacgctata aggcgttgtt ctggttatcg
    41701 ttatttcatc ttctggtgat cacctccagt aactatctgg ttcagcttcc cgtctccatt
    41761 tttggtttcc ataccacctg gggcgcgttt agctttccgt ttatttttct tgctaccgac
    41821 ctgaccgtgc gtatttttgg cgcaccgctg gcccgacgca ttatcttcgc ggtaatgatc
    41881 cctgcgttat taatctccta cgtcatctcg tcgctattct atatgggttc ctggcaggga
    41941 ttcggcgcac tcgcccactt caacctgttt gtcgcccgta tcgccaccgc cagtttcatg
    42001 gcctacgcgc tggggcaaat cctcgacgtg cacgttttta accgcctgcg tcagagtcgc
    42061 cgctggtggc tggcaccgac agcgtccaca ctgttcggta acgtcagcga cacgctggcc
    42121 tttttcttca ttgccttctg gcgtagcccg gatgccttta tggctgaaca ctggatggaa
    42181 atcgcgctgg tcgattactg tttcaaagtg ttaatcagta tcgttttctt cctgccaatg
    42241 tatggcgtat tactcaatat gctgttgaaa agactggcag ataaatccga aatcaacgct
    42301 ttgcaggcga gttaaaggtt cgttatcaga gttgtgataa gatggatgaa tgagccgtta
    42361 tggccgttta tcgaaaggaa gaagtcaatg cgcaatctgg ttaaatatgt cggaattggc
    42421 ctgctggtta tggggcttgc ggcctgtgat gataaagaca ctaacgctac ggcgcagggt
    42481 tcggttgcgg aaagtaacgc taccgggaat cccgtcaacc tgcttgatgg caagttaagt
    42541 ttctcgctgc cagcggatat gaccgaccag agcggtaagc tgggaacgca ggccaataac
    42601 atgcatgtct ggtccgacgc caccgggcag aaagcagtca tcgtcatcat gggcgatgat
    42661 ccgaaagaag atctggcggt gttggcgaag cgtctggaag atcagcaacg tagccgcgat
    42721 ccgcagctgc aagtggtaac caataaagcc attgagctga aaggtcacaa aatgcagcag
    42781 ttagacagta ttatctccgc gaaaggccag acggcgtact cttccgttat tctgggtaac
    42841 gtgggtaatc aactgctgac catgcaaatt acgctgccag ctgacgatca gcaaaaagcg
    42901 caaaccaccg cagaaaacat cattaatacg ctggttattc agtaagtttt aagatgatga
    42961 ggcggcctca gggacgtgtt ccggaggccg tttttttaat cgccacgtca gtaataacgc
    43021 gattgcgacc agtcccgccg ccgccagata aatcaccggt acgcccgccc agctcatcac
    43081 cagcccagcc agtggtccag tcacgccaag cgataaatcc ataaatacgg tgtaagttgc
    43141 cagcgccgcc ccctgatttt gctgcggaac cgcttttacc gccactacac ccaatgccgg
    43201 gaacaccagc gaaaacccgg cccccgccag taagacgccg attttcgcca tccacggcat
    43261 agtcgccacg ccaaccagta gcaggccgat tatctcaacg ctaaagcaaa tcatcgctac
    43321 gtttaagcca ccgatacggt taatgccgtt agggaataac aaacgcgtac cgacaaacgc
    43381 acagctaaac agcgtcagcg cgaaagccgc accgtcccaa cctttagcgt cataaaacag
    43441 cgtgataaag gtggcgatga cgccaaatcc ggcggaagcc agtgccagcg ccataccgta
    43501 cagccagacg cgcccaagca ccgcgcgaaa cggcagcggt ttgcctttac tggcttttac
    43561 cgtcggacgc gggatcgcca acaaaatggc caccagcgcc acgcccataa tgattaacgc
    43621 taacgcctgc aagccgcccc agtgataaaa cacgacgcct aacggcgcac ccatcgccat
    43681 cgccccgtaa gtgacaatgc cgttccacga aatcacccgc ccgatatgca gcgagccaac
    43741 cactccaacg ccccataggg tcgatcccgt tccggcaaaa ctttgcccaa tcccaaggat
    43801 gacgcgcccc aggcaaagta ataacaggct gatgacaggc agactggcgg ttaatcctgc
    43861 cgtcagatac cccagaccgc tcaaaaagca gccgcataaa ccgaagacga caatcttttt
    43921 gggtcccagc aaatcggcgt aacgtccggc atgagggcgg ctcagcaagg tggcgaaata
    43981 ttgcaggctg ataaccaatc ctgcccagaa ggcgctaaag cccatcacat catggacata
    44041 gcccggtaat acagcgagcg gcaacccgat ggtgaggtag ctggcgaagt taaacatgac
    44101 aatggagaca atgcgcaaat tcaggcgcaa tccgtttagc gcgggttcgg ctacgggttc
    44161 gggcatgagg atcaccacat ttttacaaca gtgtttcatt tttaccatgt gctgacgtga
    44221 aaatcagcag taagaatcag aatattgctg gcgtgactcc cgcgctacac ttaacacaaa
    44281 aagtcacaag gaagccccaa tggaaacccc tcaacccgat aaaacgggca tgcacattct
    44341 gctcaagttg gcctcgctgg tagtgatcct cgcgggcatt cacgcagcgg cagatatcat
    44401 tgtgcagctg ttactggcgc tgttttttgc catcgtcctc aacccgctcg ttacctggtt
    44461 tattcgtcgg ggagtacaac gccccgttgc cattacgatt gtagtggtgg tgatgctgat
    44521 cgcactaacc gcgctggtcg gcgtactggc ggcatcgttt aacgaattta tctctatgct
    44581 gccgaagttt aataaggagc tgacgcgcaa actttttaaa ttgcaggaga tgttgccttt
    44641 tcttaatttg catatgtcgc cggagcgaat gctgcagcgg atggactcgg aaaaagtggt
    44701 taccttcacc acagcgctaa tgaccgggct ttccggggca atggcgagcg tgcttttgct
    44761 ggtgatgacc gtagttttta tgctgtttga agtgcgccac gtcccttaca aaatgcgttt
    44821 tgcgctgaat aacccacaga ttcacatcgc aggattacac cgcgcactta aaggcgtttc
    44881 gcattatctg gcgttaaaga cgctactcag tttatggaca ggtgtcatcg tctggctggg
    44941 gctggcgcta atgggcgtac agtttgcgct gatgtgggca gtactggcgt ttttgctcaa
    45001 ctacgtgccc aatatcggcg cggtaatttc cgccgtaccg ccaatgattc aggtgctgct
    45061 gtttaatggt gtttacgaat gtattctggt cggcgcattg tttttagtgg tccatatggt
    45121 catcggcaat attttagaac cacggatgat gggccatcgc ctggggatgt ccaccatggt
    45181 ggtatttctt tcattgttaa tctggggatg gctgctcggc ccggtaggga tgctactttc
    45241 ggtgccatta accagcgtgt gtaaaatctg gatggaaacg acgaaaggtg gcagcaaact
    45301 ggcgatttta ctggggccgg gcagaccgaa aagtcggtta ccgggatgag gcgacaagtg
    45361 atacgatacg cactttcatt ttccattaaa cgttggccct gatatgtatc ggatagttct
    45421 ggggaaagtt tcgaccttaa gcgcagctcc actgccaccg ggtttacgcg agcaagcacc
    45481 gcaaggtcca cgacgcgaac gctggctggc ggggcgtgca ttgctttcgc acacgctttc
    45541 cccgctaccg gagatcatct atggcgaaca aggcaaacct gcattcgcgc cggaaacgcc
    45601 gctatggttc aacttaagcc atagcggtga cgatatcgct ctgctgttga gcgatgaagg
    45661 cgaagtcggc tgcgatatcg aagtgattcg cccgcgcgcc aactggcgct ggctggcgaa
    45721 cgccgtgttc agcctcgggg aacacgctga aatggacgcc gtgcatcctg atcagcaact
    45781 ggaaatgttc tggcgcatct ggacgcgcaa agaagccatc gttaaacagc gtggcggcag
    45841 cgcctggcaa atcgtcagcg tagatagcac ctatcactcc tcgctgtcag tcagccattg
    45901 tcagctagaa aatttaagcc ttgcgatctg cacccccacc ccctttacgc tcaccgccga
    45961 cagtgtgcaa tggatcgatt cagttaactg atccgcccac ccgactgccc atctattgat
    46021 ccagaacagg taatcagtat gacgaatact taaaatcgtc atacttattt ccgccatcta
    46081 ttttaatcca ttggggttac catgctctcc acactccgcc gcactctatt tgcgctgctg
    46141 gcttgtgcgt cttttatcgt ccatgccgct gcaccagatg aaatcaccac cgcctggccg
    46201 gtgaatgtcg ggccactaaa cccgcacctt tacacgccta accagatgtt cgcccagagc
    46261 atggtttatg aaccattggt gaaatatcag gcagacggtt cggtgatccc gtggctggca
    46321 aaaagctgga ctcattcaga agatggtaaa acctggacct tcaccctgcg tgatgacgtg
    46381 aaattctcca acggtgaacc gttcgatgcc gaggcggcgg cagaaaactt ccgcgcagtg
    46441 ctcgataacc gtcaacgtca cgcctggctg gagctggcaa accagattgt tgatgttaaa
    46501 gcactcaata aaacagagct gcaaattacc ctgaaaagcg cctactatcc tttcctgcaa
    46561 gaactggccc tgccccgtcc tttccgtttt atcgctccct cgcagtttaa aaaccatgaa
    46621 accatgaacg gaattaaagc gccgattggc accggaccgt ggattttgca ggaatcgaaa
    46681 ctgaatcagt acgatgtctt cgtccgtaac gaaaactact ggggcgaaaa gccagcgatt
    46741 aaaaagatca cctttaacgt catcccggac ccgactaccc gcgcggtggc gtttgaaact
    46801 ggcgatatcg acctgctgta cggaaacgaa gggttattac cgctcgatac cttcgcccgc
    46861 tttagccaga atccggctta ccacacccaa ctgtcacagc cgatcgaaac cgtgatgctg
    46921 gcgctcaata ccgccaaagc ccccaccaac gagctggcag tacgtgaagc tcttaattac
    46981 gcggtaaaca aaaaatcgct gattgataac gcgttatatg gcacccagca ggtcgccgac
    47041 accctgtttg ccccttctgt gccttacgct aatctcggcc tgaaaccgcg ccagtacgat
    47101 ccgcagaaag cgaaagagtt gctggaaaaa gccggttgga cgctgcctgc gggcaaagac
    47161 atccgcgaga aaaatggtca gccgctgcgc attgaactgt cgttcatcgg caccgatgcg
    47221 ttaagcaaat cgatggcgga aatcattcag gctgatatgc gccagattgg cgcagatgtc
    47281 tcgctgattg gcgaagaaga gagcagtatc tatgctcgtc agcgcgacgg tcgttttggc
    47341 atgattttcc accgcacctg gggcgcgcca tacgatccac acgccttcct gagttcaatg
    47401 cgcgtaccgt cgcacgctga cttccaggca cagcaaggat tagccgacaa gccgctgatt
    47461 gataaagaga tcggcgaagt gctggcgacc catgacgaaa cgcaacgtca ggcgctgtat
    47521 cgcgacattc tgacccgtct gcatgacgag gcggtttatc tgcctatcag ctacatctcg
    47581 atgatggtgg tatcaaaacc ggagctgggt aatatcccct acgcgccgat cgccaccgaa
    47641 attccgttcg aacagattaa accggtgaaa ccttaatgtt gcgttacgta ttacgccgct
    47701 ttctgctgct gatcccgatg gtgctcgccg cctcggtgat catttttctg atgctgcgcc
    47761 tcggtaccgg cgacccggcg ctcgattatc tgcgtttatc taacctgccg ccgacgccgg
    47821 agatgctggc ctctacccgc accatgctcg ggctggatca gccgctgtac gtccagtacg
    47881 gtacctggtt gtggaaggcg ctgcatcttg actttggtat ctcattcgcc agccaacgcc
    47941 cggtactgga cgatatgctg aacttcctgc ccgccacgct ggaacttgca ggtgcggcgc
    48001 tggtattaat tctgctcact tccgtaccgc tcggtatctg ggcggcgcgc caccgcgatc
    48061 gtttgccgga tttcgccgtg cgctttatcg cgtttcttgg cgtgtcgatg cccaacttct
    48121 ggctggcatt tttgctagtg atggcgtttt cggtgtatct gcaatggcta cccgcgatgg
    48181 gctacggcag ctggcagcac atcattttac ctgcggtttc catcgccttt atgtcgctgg
    48241 cgattaacgc gcgactgctg cgcgccagta tgctggacgt cgccggtcag cgtcacgtca
    48301 cctgggcgcg gctacgcggc ctgaatgaca aacagaccga acgccgccat attctgcgca
    48361 atgcctcgct gccgatgatc accgccgtgg ggatgcacat tggcgaattg attggcggga
    48421 cgatgattat cgaaaacatc tttgcctggc ctggcgtcgg gcgctatgcg gtgtcggcga
    48481 tttttaaccg tgactatccg gtgattcagt gctttacgct gatgatggtg gtggtttttg
    48541 tggtctgtaa tttgattgtc gatttgctca acgccgcgct ggacccgcgc attcgtcgtc
    48601 atgaaggagc gcacgcgtga actttttcct ctcttcccgc tggtcggtac gcctggcgct
    48661 gatcattatc gccctgctgg cgctgattgc gctcaccagc cagtggtggc agccgtacga
    48721 tccacaggca attgatttgc cgtcgtgcct gctttcgccg gatgcgcagc actggctggg
    48781 caccgatcac ttaggtcgcg atattttctc gcggctgatg gcagcgaccc gcgtgtcgct
    48841 cggttcggta atggcctgcc tgctgctggt gctgacatta gggctggtta ttggcggcag
    48901 cgccgggttg attggcgggc gcgttgatca ggccaccatg cgcgtcgccg atatgtttat
    48961 gaccttcccg acctcgattc tgtcgttctt tatggttggc gtgctcggca ccgggctgac
    49021 caacgtaatt atcgccatcg ccctgtcgca ctgggcgtgg tatgcacgca tggtgcgcag
    49081 cttggtgatt tcactacgcc aacgcgagtt tgtgctggcg tcacggcttt ccggtgcggg
    49141 ccatgtgcgg gtgtttgtcg atcatctggc aggcgcggtg atcccttcgc tgctggtgct
    49201 ggcaacgctg gatatcggcc atatgatgct gcacgtcgcg gggatgtctt tccttggcct
    49261 cggtgtgacc gcgccgaccg ccgaatgggg cgtgatgatt aacgacgcgc gccagtatat
    49321 ctggacccag ccgctgcaaa tgttctggcc ggggctggcg ctgtttatca gcgtgatggc
    49381 ctttaacctg gtgggtgacg cactgcgcga tcatctggat cctcatctgg tgacggagca
    49441 cgcacactaa tgccgcaaca gattgaacta cgtaatatcg cgctacaggc cgcgcagccg
    49501 ctggtacacg gtgtatcgtt aaccctgcaa cgcgggcgcg tgctggcgtt agtcggcggt
    49561 agcggcagcg ggaaatcatt aacctgcgcc gcgacgctgg gcattttgcc cgctggcgtt
    49621 cgccagacgg cgggggaaat tttagccgat ggcaaaccgg tttcgcctta cgccctgcgc
    49681 ggcatcaaaa ttgccaccat catgcagaac ccgcgcagcg cctttaatcc actgcacacc
    49741 atgcacaccc acgcgcggga aacctgcctg gcgttaggga aacccgccga tgacgctacg
    49801 cttaccgctg ccatagaagc ggtggggctg gaaaacgccg cgcgcgtgct gaagctgtac
    49861 ccgttcgaga tgagcggcgg catgttgcag cgcatgatga ttgcgatggc ggtgctgtgt
    49921 gaatcaccgt ttatcatcgc cgatgaaccg accaccgacc tcgacgtggt agcacaggcg
    49981 cgcatcctcg atctgctgga aagcattatg caaaaacaag cgccgggaat gctgctggtc
    50041 acccatgata tgggcgttgt ggcgcgtctg gcggatgacg tggcggtgat gtcacaaggt
    50101 aaaattgtcg aacagggcga tgtagaaacg ctgtttaacg cccccaaaca tacggtgacg
    50161 cgcagcctgg tttccgctca tctcgccctc tacggtatgg agctggcatc atgaccttac
    50221 ttaacgtctg cggcctttcc catcactatg cccacggtgg atttagcgga aaacatcaac
    50281 atcaggcggt gctgaataac gtttccctga ccctgaaaag cggcgaaacc gtcgccctgc
    50341 tgggacgcag cggctgcgga aaaagcaccc tcgcgcggct gctggtaggc ttagagtctc
    50401 cctcacaagg gaatattagc tggcacggcg aatcgctggc gaaacttaat cgcgcccaac
    50461 gtaaagcctt ccgccgtgat attcagatgg tgtttcagga ctccatcagt gccgtgaatc
    50521 cgcgcaaaac cgtgcgcgag atcctgcgtg aaccgatgcg ccacctgcta tcactgaaaa
    50581 aatccgaaca actggcgcgc gccagcgaaa tgctgaaggc ggtcgatctc gatgacagcg
    50641 ttctcgacaa acgcccaccg cagttaagcg gcggccagct ccagctcgtc tgcctggcac
    50701 gcgcgctggc ggtcgaaccg aaattgctga ttctggatga agccgtttct aaccttgatc
    50761 tcgtgttaca ggcgggcgtc attcgcctgc tgaaaaagct acaacaacag tttggcactg
    50821 cctgcctgtt catcactcat gacttacgcc tggtggaacg cttttgccag cgagtgatgg
    50881 tgatggacaa cggacaaatc gccgaaaccc aggcggtggg agataaatta accttttcct
    50941 ctgacgccgg acgtgtgcta caaaacgcgg tattacccgc attccccgtg cgccgtcgca
    51001 cctcagaaaa ggtttaacgc caatgcaacg agtcaccatc acgcttgatg acgatttact
    51061 ggagacgctg gacagcctga gccagcgtcg tggttataac aaccgttccg aagctatccg
    51121 cgatattctg cgtagtgccc tggcgcagga agctacccag cagcacggca cacaaggttt
    51181 cgcggtgctg tcgtatgtgt atgaacacga aaaacgcgac ttagccagcc gtattgtttc
    51241 cacccagcat catcaccacg acctctccgt cgccacgcta catgtgcaca tcaaccacga
    51301 cgactgtctg gaaatcgccg tgttgaaagg tgacatgggt gacgtgcagc attttgccga
    51361 tgacgttatc gcccagcgcg gcgtgcggca cgggcatttg cagtgcctgc cgaaggaaga
    51421 ttgagttatt tctaaaacaa ggaaagaaaa gatattaact gaacttaatt tataaaatta
    51481 agttcagttg catagataga tcctgtaaag gcagatattc tgcgtaatgt acgtcgctaa
    51541 aatttataaa tgattcaaac gctcattcag tgtttcaata atataggtat taacagagac
    51601 ttgttgctgc gcagccgcat tattaaggcg ttcactcagc gattctggat agcgtaacgt
    51661 aaaggtctta atcttttctg tacgcgcata cggctcaata cccgcagctt tacaatcctc
    51721 aagatactct cgcagggaaa gctctccttc tttttgcagc ccctgaatac tgtcagagac
    51781 aaaatcacaa taacctgaca agcccaggaa tttgccacga aatgcattca gttctggcac
    51841 gtaggtaata acagctggtt gcccggcaat ctccatagag tttggcgtct tcagtttgat
    51901 catggtttta cccctatact tagtaaccag tcacacacgc tttctacagc gcctttatcg
    51961 gtatctggcg acggatgagg ccgatggaaa cacgcaacgc tcatatttag tatgaacttg
    52021 caacgcgaac ctcttccttt cttaatttct ccgcctaatg ctttaaccag tgactctata
    52081 tcagaccact tgatcccttg aggaacagga gttttaaata tttgatccaa tgtatttttt
    52141 tgtttcttcc gtaaagcctg taccttggtt ctcatggcat gtccttatga cttcacttta
    52201 tgacatcaca gtatgaagtc aagtttccct tccttacgcc attgtcccaa tcgtcttcct
    52261 gaatcgcagc agtgcgatgg tgaaaaacgc gccgccaatt gccatcagcg tcaggaactg
    52321 cggccagacg atttcgaatc cggcaccccg gtagaggatg gcctgcgcga ggctaacaaa
    52381 gtgtgtcgtc ggcatggtca gcataatgtc ctgcaccatc tgcggcatac tttcgcgcgg
    52441 cgtggatcca ccggaaagca tttgcagcgg cagcagcacc agaatcacca gcagccccag
    52501 ttgcggcatt gaacgcgcta tcgtccccat aaaaatgccg attgacgtgg tggcaaacag
    52561 actgagcgcc acgcccagca taaacagcgg gatcgagcct tcaatcggta cgcccagtac
    52621 acctttcacc atcagcacca gcgataatcc cgataccacc agcaccacca gccccatcga
    52681 ccagatcttc gccatcatga tctcaaacgg cgttatcggc atcaccagta aatgttccac
    52741 cgtgccgtgt tcacgctcgc ggatcagcgc cgatccggtc aatacaatcg ccagcatggt
    52801 aatgttgttg atgatcgcca tcaccccgcc aaaccacgcg ggatcaaggt tcgggttaaa
    52861 gcgcatccgg gtttccagcg ataccaacgg ttcgctgtta tcacggtagc gcgcgacaaa
    52921 gctgttcact tcaccgttga taatattctg gatataccca ttgccggtaa atgcctggct
    52981 catgcgcgtg gcatcgacgt tcacctgaat atccggctgg cgtccggcga ggacatcacg
    53041 ctgaaaatta ggcggaatat ttatcgcgaa ggtatagcgt ccggcgtcca gtccggcatc
    53101 catctcatcg gcggtgatca tctccggtgg caaaaaccac ggacgataga agctgttaac
    53161 gatccggttc gataactgcg attgatccat atcggcaatg gcgatcggcg cgaggttcaa
    53221 cgatcctggc gtaacggtcg ctgacgaata caccgacacc gtaaacgaga agacaatcag
    53281 cgtcagcatc gctttatcac cgagcagact gcgcaactct ttgataccca gattaaaaat
    53341 attgcgtaaa tggcgcatca tccctcctgt tttttcagca gcaggatgct taagcccatc
    53401 accagcggga tggctatcag caacgggata aaaagttgcc acaaatcagt cagatccagc
    53461 gctttcgaga acgttccgcg ggcgatagtc agaaaatgac tggtcgggta aacctcgccg
    53521 atccaacgtc caagcccttc cagcgaagct accggatcga tcatcccgga aaactgtgtc
    53581 gccgggatca atgtgataat cgccgttccg aaaatggcgg caatctggct tttcataaag
    53641 gtggagatca gcagccccat tccagtggca atgatgatat acagcagcgc cgccaaggtg
    53701 agcgtcagga aactgccttt atgcggtacg ccaaacacaa acaccgacag gccgcagagc
    53761 aggaaaaagt tcagcatccc cagcgcgatg tacggcagct gtttgccaag caaaaattcg
    53821 ctacgggtgg tgggggtcac gtaaaggttg ataatcgacc ctagctcttt ttcccgcacc
    53881 acgctaaggg cgcttagcat tgacgggatc atcatcagca gaagcgggat caccgccgga
    53941 acaatcgctg gcaggctttt tacgtccggg ttatagcgat aacgcgtctc aatgttcatc
    54001 agcccgcttt ggctggcggg tgtcgattgt cggctcgcca catcctgaag ccaactctgg
    54061 tgcattgcct gcacgtaacc ttttaccgtt tcggcacgac tgggcatcgc tccgtcgatc
    54121 cagacgccga gttccacagg cgtaccacgc gcgatatcgc gcccgaaatt gggcgggatc
    54181 tcaatggcca ccgtgatatc gcccgcacgc atccgacgat caagctcgtc ataactggtg
    54241 agcggcggct gttcgataaa gtaacgggaa ccggagaggt tgagtgtcca cgcctggcta
    54301 ctgacggtct ggtcgcggtc gagcaccgca aagcgcaggt tttccacatc catactgatg
    54361 ccgtaaccca ttatcagcat caggatcacc gttcccatca gcgccagcgt cgaacgtact
    54421 ggatcgcgtc gcagttccag cgcttcgcgg cggctgtagc taaacagacg gcgcaggcta
    54481 aatccctgac gcggcgcgtg ggtggtgtcg tgtaccacgg gcggcgcttc ggcttcgttg
    54541 ctctgccctg ccgcttcctg caaataggcg ataaatgcct cttccagact ggcggctccg
    54601 cgtttctcaa ccagttcctg cggtgtaccg ctggcaagca cttttccggc gtgcatcagt
    54661 gagatgcggt cgcaacgttc cgcttcgttc ataaagtggg tggagatgaa gatagtcact
    54721 ttgtcctggc gcgagagatc gaccatcaac tgccagaaca tatccctcgc caccggatcg
    54781 acaccagaag taggctcatc gaggattaac atctccgggc gatgaatcac cgccaccgcc
    54841 agcgaaagcc gctggcgaat gccgagcggc aatgactccg gcagaacatc ttcaacgtcg
    54901 ttgagcttaa aacgctcgct catttcagcc actcgcgcgg gaatttccgc ttccgggatg
    54961 tgaaacaaac gggcatgtaa ctccaggttt tgccgcacgg tgagttcgtt atagagcgaa
    55021 aacgcctgcg acatatagcc cacacgacgg cgggtatcga tatcttttgg atcaaccggt
    55081 tgcccaaata gccacgcctc accttcgctg gcgggcagca gtccggtaag cattttcatg
    55141 gtggtggatt taccgcagcc gttcgaacca aggaatccaa aaatctcccc gcgtggaatg
    55201 cggaaattga cgtgatcaac ggcaacgaag gaaccaaaac gcatggtcag atcgcgcgct
    55261 tcgatggcaa tctctgcgtt ttcaggttga tacggtggga tcactaccgc ctgatgcgcc
    55321 tggcgttgcg cttgcggtaa cagattaata aatgcttctt ccagcgtggc gctttgcgtt
    55381 tgctgccgca gctcttcggc gctgccggtt gccagcactt ctccggcatt catcgctacc
    55441 agccagtcga agcgttcggc ctcttccata taggcggtgg cgaccagcac gctcatattg
    55501 ctctgccgct ggcgaatact gtcgatcaga tcccagaact gggcgcggga gagcgggtca
    55561 acccccgttg ttggctcatc aaggatcaac agttccgggt cgtggattaa cgcgcagcac
    55621 agcccaagtt tttgcttcat cccgccggag agtttacctg ccggacgatc gcgaaacggt
    55681 gctaacccgg tgctggtcag cagctcatta attcgcactt cccgctccgc tttatcgtga
    55741 ccgaacaggc gagcgaaaaa atcgacattt tcatacaccg acaaggtgtg gtagaggttt
    55801 ttgcccagcc cctgcggcat ccaggcgatg cgcgggcaga cgtctcggcg atgcttcggg
    55861 tcgtgcatat cgccacccag caccaccaca ttgccctgct caatgacgcg ggcaccggaa
    55921 atcagtgaca ataaactcga cttcccgacg ccatccggac caatcagccc gaccatgcaa
    55981 cgggccggaa tatcgagagt gatattgttc agcgcaacgg tttttccata atgctggctc
    56041 acgcccgcca gttgcgcgac aggcgggacg ggaaccagtt ccagatgcgt cattgcggca
    56101 acctcaccac gaggtcgtca ggccacggaa gttcttcatt cacccgcacc cacgctacgc
    56161 ccggtaaacc ggttttgaca tattccagat gctgctggag taattccggt gggatacgcg
    56221 ctttgacgcg gaacatcagt ttcagccgtt catcgctggt ttcgacggtt tttggcgtga
    56281 actgggcgac actggcgaca aaactgatgg ttgcaggaat acgcagatct ggcgcggcat
    56341 cgaggatcag ccgggcttca ccgcccagtt tcagcgtgcc cgcctgttcg gttggcagga
    56401 agaaggtcat atagacgtcg ctgagatcga ccatattcag cacccgaccg cctgccgcca
    56461 gcacttcccc tggctcggca acccgatact gcacgcgtcc gtcacgcggg gctttcagtt
    56521 cactgtcatc gatatctgcg gcaatgcgcc gttcagtggc ttgcgctgct tcgacccgag
    56581 tttgcgcctg aatgatattg gtgcgtgctg cttctatagc cgctttagaa gccgaaacct
    56641 gggctttcgc tgattccagc gcagctcggg cgctctcagc ggcggcgcgg tcgtcatcca
    56701 gctgttgcgc agaaatagcc cctcgttggg cgagcgagcg ggaacgcgta tgacgtttgg
    56761 caacagagtc cagttcggcc tggcgttgat tgaccagcga ctgtgcggca cgagtttcac
    56821 tttgtcgttg ctccagcaaa gcctgcgcgg cggcaacggc gctttgtgcc tctttgattt
    56881 gcgcgatggc ttccagtcgc tgttcctgca acacgcgagt atccatcttc gccagcactt
    56941 cgccttcgcg aacaaactgg ccttctttca ccagaatggt gtcgatacgc ccggcaattt
    57001 tgctggcgat atccacttcc gtcgcttcaa tgcgcccatt gctgacagca aagccttccg
    57061 gcacacccgc cgggcgcaac agccaccacg ccacgacggc agccaccgcc agtgccccga
    57121 caacccacca cgccagatgg cgcttactct tatccataat cgacccgcca taatccctgt
    57181 aagcaaacga cgcgtgttac aactgccgcc gaacgacgtt cagcggattc ttccatccgg
    57241 gataagaagg aaacgacaaa tacgaccagc gcgcagagca ttgagcgcca acggggaagg
    57301 cttagtgaaa acgggtagta atgttgtcat catcagcatt cctggccgta aatgaaacac
    57361 gcatactcat aagtgtgatc tataaaactg gctgacaaaa cggtatccat cctgttggat
    57421 cgcatttcac gcctgttcag ttgcaggaac agagcattcc tttttacgtt ttatcaacag
    57481 tctcaaccag atagtaacat taatattcat gttgtctatg gttcagagct gtaacattgt
    57541 gcgccttcta atcaaaggca taagtccatt tctgtttttt tcatgctggt aagacttcca
    57601 gggagtggtt atctctggcc ctgcaccagg gttaatttgg caattatgca tgctgaacat
    57661 tcttccagca tgtttactgt cacgggcgtt aagacctaaa actgtccccg tttcacctat
    57721 gcgttctctc ccaccatttc cgttatgtgc aacaacttga tttataccta acatttgtga
    57781 acatgctaat agtgcttcaa gtttaggtct gtaatctgtc gaaacagaaa aataacgatt
    57841 aaaggatcga caagagatag cccggctagt tttaaacttt ttgccattaa ttatagcatg
    57901 atgcttttta ttaataattt ccgctaattc gaccggatcg agagcattgg tcatttcatc
    57961 aatagagaca gttatcggtc ctataatgta acgttttcgc tgatcatcaa aagttatccc
    58021 atgatgattt gccattattt tgtatttggg gtcatagaaa catacaggat aatcttttat
    58081 cccgtcgtag gtatcctcat caagtggttt catgcgcgtg aagttttgca tatagttacc
    58141 attgcaattc gtttcatgat ttccagctaa tacatgaaca ttttcattga tactcattat
    58201 gcgttttaac aaatcgatga taaattgatc gccacagata ttactaaatc gatcgccggt
    58261 ttggtcgcca agaaacgtac atggcgtgac tgcgtcttta ttaataacca aataaggctg
    58321 aagactttct aaaacattat ctcttctgtt cagtatactg attattgtat taacattagt
    58381 gtcaagttgc tcattaaagt tctcccttag caatacgttg agaacttcat cttctatatt
    58441 aagcaactgg ctaaggaaat tttctccttc agccatgatt cccaaatgtc ctgttgcaaa
    58501 aagcgcgtac aaaactgcac cgactgagcc atcggtatcg ccaaaatagg tcggcttggt
    58561 acttgtggga tcaacaggtg aacaattgga gaaataggtt ctgtcagcga actggggttt
    58621 tataattgtt tcgaatttaa tatcaaatac attgtattgc gatatttccc agttccagat
    58681 gggtttgtac gactcagtaa ctgcaacatc attagcatta tcatttactg tcatattatt
    58741 aagtaaataa acaatgctgc accaattaat atcggtatta tcaagagggc aaccgtaatc
    58801 tttactatta attgtcacaa taaactgata acagttattc ctacgtagaa tatgagcgcg
    58861 catgcccgat agcatactat atttttctga atgattcgtt tgtatggtaa atgaaatttt
    58921 gtcatcttga atagattgtt ggaaacgttc tatttgccaa tcatcaattt tttccacaac
    58981 ttcaccgagc attgtcctgg caatacttga tatatcatca cctataccat ttgcaatata
    59041 cccaaacagt tctttagtga agttggtaac atgctcttgc accatatcat ttgttgcgat
    59101 tgtcgttgta ctgccattag tgcctgccac agttccaatt ttcatctttt acctcatatt
    59161 attcaccgcg aggaatacat agttaacatt ttatcttatg acatacacct catattcgat
    59221 ggggaaataa ttatattttg cacaaatcat tcagccttaa agcataatat gaaatttctt
    59281 atctcatagc agagaaagtc cctaaagatt gtagaggcgt catcagtaca tacctctaca
    59341 atcgattaag aaaacctatg aaatactggc aaaattaaaa agagatacca cagcactcat
    59401 gaagttttat tgaggttgaa aacaactaag ccctgggaca ccttttttat caccacgttc
    59461 gttaggtgct atcttccaag aatttagccc catggtataa ttatcaattt tttgatgctg
    59521 gtctacccct aatccataat ggccaaattt c
//
LOCUS       DAJTQY010000014        58601 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.014, whole genome shotgun sequence.
ACCESSION   DAJTQY010000014 DAJTQY010000000
VERSION     DAJTQY010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 58601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58601
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.014"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(<1..1128)
                     /locus_tag="OO349_000874"
     CDS             complement(<1..1128)
                     /locus_tag="OO349_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HCR5866047.1"
                     /translation="MKQKITDYMDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPIFNQFYHQWLQSIFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQLPGFEDFFIAVDP
                     KTDLSAVTLPRALPLLTPFQMDDNSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LEKGAEYVRLDAVGFMWKEPGTSCIHLEKTHLIIKLLRSIIDDVAPGTVIITETNVPH
                     KDNIAYFGNGDDEAHMVYQFSLPPLVLHAVQKQNVEALCQWAQSLSLPSSKTTWFNFL
                     ASHDGIGLNPLRGLLPESEILALVEALQQEGALVGNAANLLI"
     gene            complement(1340..1687)
                     /gene="pspE"
                     /locus_tag="OO349_000875"
     CDS             complement(1340..1687)
                     /gene="pspE"
                     /locus_tag="OO349_000875"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HCR5866048.1"
                     /translation="MAIFIHRRTLTCLKKGLLALALVFSLPVFAAEHWIDVRVPEQYQ
                     QEHVQGAINIPLKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKELLSEMGYTHVENA
                     GGLKDIAMPKVKG"
     gene            complement(1730..1951)
                     /gene="pspD"
                     /locus_tag="OO349_000876"
     CDS             complement(1730..1951)
                     /gene="pspD"
                     /locus_tag="OO349_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HCR5866049.1"
                     /translation="MNTRWQQAGQKVKLGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(1960..2319)
                     /gene="pspC"
                     /locus_tag="OO349_000877"
     CDS             complement(1960..2319)
                     /gene="pspC"
                     /locus_tag="OO349_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HCR5866050.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(2319..2543)
                     /gene="pspB"
                     /locus_tag="OO349_000878"
     CDS             complement(2319..2543)
                     /gene="pspB"
                     /locus_tag="OO349_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HCR5866051.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLVDEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(2597..3265)
                     /gene="pspA"
                     /locus_tag="OO349_000879"
     CDS             complement(2597..3265)
                     /gene="pspA"
                     /locus_tag="OO349_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HCR5866052.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            3432..4409
                     /gene="pspF"
                     /locus_tag="OO349_000880"
     CDS             3432..4409
                     /gene="pspF"
                     /locus_tag="OO349_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HCR5866053.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEEAIAVSENTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(4529..5794)
                     /gene="puuE"
                     /locus_tag="OO349_000881"
     CDS             complement(4529..5794)
                     /gene="puuE"
                     /locus_tag="OO349_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HCR5866054.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINTLAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGECGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSTIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(5832..7112)
                     /gene="puuB"
                     /locus_tag="OO349_000882"
     CDS             complement(5832..7112)
                     /gene="puuB"
                     /locus_tag="OO349_000882"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HCR5866055.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDVNAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTANGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARNPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSHMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(7114..8601)
                     /gene="puuC"
                     /locus_tag="OO349_000883"
     CDS             complement(7114..8601)
                     /gene="puuC"
                     /locus_tag="OO349_000883"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HCR5866056.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQKAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDSAHADSVHTFIREGEGKGQLLLDGRNAGLAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(8751..9308)
                     /gene="puuR"
                     /locus_tag="OO349_000884"
     CDS             complement(8751..9308)
                     /gene="puuR"
                     /locus_tag="OO349_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HCR5866057.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(9335..10099)
                     /gene="puuD"
                     /locus_tag="OO349_000885"
     CDS             complement(9335..10099)
                     /gene="puuD"
                     /locus_tag="OO349_000885"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HCR5866058.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPSSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITAWQHHIAEKQRL"
     gene            10311..11729
                     /gene="puuA"
                     /locus_tag="OO349_000886"
     CDS             10311..11729
                     /gene="puuA"
                     /locus_tag="OO349_000886"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HCR5866059.1"
                     /translation="METNIVEVENFVQQSEERRGSTFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGDLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYHVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            11878..12042
                     /locus_tag="OO349_000887"
     CDS             11878..12042
                     /locus_tag="OO349_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5866060.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            12032..13417
                     /gene="puuP"
                     /locus_tag="OO349_000888"
     CDS             12032..13417
                     /gene="puuP"
                     /locus_tag="OO349_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HCR5866061.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            13551..13796
                     /locus_tag="OO349_000889"
     CDS             13551..13796
                     /locus_tag="OO349_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HCR5866062.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            14109..15752
                     /gene="sapA"
                     /locus_tag="OO349_000890"
     CDS             14109..15752
                     /gene="sapA"
                     /locus_tag="OO349_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HCR5866063.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            15749..16714
                     /gene="sapB"
                     /locus_tag="OO349_000891"
     CDS             15749..16714
                     /gene="sapB"
                     /locus_tag="OO349_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HCR5866064.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKELFPATIELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            16701..17591
                     /gene="sapC"
                     /locus_tag="OO349_000892"
     CDS             16701..17591
                     /gene="sapC"
                     /locus_tag="OO349_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HCR5866065.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            17591..18583
                     /gene="sapD"
                     /locus_tag="OO349_000893"
     CDS             17591..18583
                     /gene="sapD"
                     /locus_tag="OO349_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HCR5866066.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKK"
     gene            18585..19391
                     /gene="sapF"
                     /locus_tag="OO349_000894"
     CDS             18585..19391
                     /gene="sapF"
                     /locus_tag="OO349_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HCR5866067.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            19459..19812
                     /locus_tag="OO349_000895"
     CDS             19459..19812
                     /locus_tag="OO349_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HCR5866068.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            20180..20968
                     /gene="fabI"
                     /locus_tag="OO349_000896"
     CDS             20180..20968
                     /gene="fabI"
                     /locus_tag="OO349_000896"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HCR5866069.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            21112..22239
                     /locus_tag="OO349_000897"
     CDS             21112..22239
                     /locus_tag="OO349_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HCR5866070.1"
                     /translation="MEQRHITGKSHWYHETQSSTTEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTVWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLAIQNYADASLFADESIRWRSSYEV
                     EKLPEEHTKSSTAELCQLAETHSLHPISLSLLEKLLNSTRVNAQPDNQLAALLCARIN
                     GSPACFSTCMDSSNEYKKISTLLRKGENEINRWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            22307..24241
                     /gene="rnb"
                     /locus_tag="OO349_000898"
     CDS             22307..24241
                     /gene="rnb"
                     /locus_tag="OO349_000898"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HCR5866071.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLTIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTSLDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTTEDNIEFFAATIESKAKLVYDQVSDWLENTGDWKPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISSEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTGTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(24319..24421)
                     /locus_tag="OO349_000899"
                     /pseudo
     CDS             complement(24319..24421)
                     /locus_tag="OO349_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            24475..26449
                     /gene="pdeR"
                     /locus_tag="OO349_000900"
                     /pseudo
     CDS             24475..26449
                     /gene="pdeR"
                     /locus_tag="OO349_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
     gene            26597..26770
                     /locus_tag="OO349_000901"
     CDS             26597..26770
                     /locus_tag="OO349_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5866072.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKTPWEI"
     gene            26860..27609
                     /locus_tag="OO349_000902"
     CDS             26860..27609
                     /locus_tag="OO349_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR5866073.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            27878..28096
                     /gene="osmB"
                     /locus_tag="OO349_000903"
     CDS             27878..28096
                     /gene="osmB"
                     /locus_tag="OO349_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HCR5866074.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(28222..28548)
                     /gene="yciH"
                     /locus_tag="OO349_000904"
     CDS             complement(28222..28548)
                     /gene="yciH"
                     /locus_tag="OO349_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HCR5866075.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGAVRIQRQTSGRKG
                     KGVCLITGIDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(28548..29285)
                     /gene="pyrF"
                     /locus_tag="OO349_000905"
     CDS             complement(28548..29285)
                     /gene="pyrF"
                     /locus_tag="OO349_000905"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HCR5866076.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNCDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CDLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(29478..30647)
                     /gene="lapB"
                     /locus_tag="OO349_000906"
     CDS             complement(29478..30647)
                     /gene="lapB"
                     /locus_tag="OO349_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HCR5866077.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYSCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(30654..30962)
                     /gene="lapA"
                     /locus_tag="OO349_000907"
     CDS             complement(30654..30962)
                     /gene="lapA"
                     /locus_tag="OO349_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HCR5866078.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            complement(31111..31875)
                     /gene="pgpB"
                     /locus_tag="OO349_000908"
     CDS             complement(31111..31875)
                     /gene="pgpB"
                     /locus_tag="OO349_000908"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HCR5866079.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            32045..32635
                     /gene="ribA"
                     /locus_tag="OO349_000909"
     CDS             32045..32635
                     /gene="ribA"
                     /locus_tag="OO349_000909"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HCR5866080.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(32699..35374)
                     /gene="acnA"
                     /locus_tag="OO349_000910"
     CDS             complement(32699..35374)
                     /gene="acnA"
                     /locus_tag="OO349_000910"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HCR5866081.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSMLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELEVNATHKDRLPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGEPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(35538..35633)
                     /locus_tag="OO349_000911"
     CDS             complement(35538..35633)
                     /locus_tag="OO349_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5866082.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(35747..35914)
                     /locus_tag="OO349_000912"
     CDS             complement(35747..35914)
                     /locus_tag="OO349_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5866083.1"
                     /translation="MVGQEQLESSPLCQHSDNEPETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(35917..36081)
                     /locus_tag="OO349_000913"
     CDS             complement(35917..36081)
                     /locus_tag="OO349_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HCR5866084.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVV
                     VALLIWKVWG"
     gene            complement(36376..37350)
                     /gene="cysB"
                     /locus_tag="OO349_000914"
     CDS             complement(36376..37350)
                     /gene="cysB"
                     /locus_tag="OO349_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HCR5866085.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGPPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            complement(37560..40157)
                     /gene="topA"
                     /locus_tag="OO349_000915"
     CDS             complement(37560..40157)
                     /gene="topA"
                     /locus_tag="OO349_000915"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HCR5866086.1"
                     /translation="MGKALVIVESPAKAKTINKYLGCDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILCFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            40537..40788
                     /locus_tag="OO349_000916"
     CDS             40537..40788
                     /locus_tag="OO349_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HCR5866087.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            complement(40824..41873)
                     /gene="sohB"
                     /locus_tag="OO349_000917"
     CDS             complement(40824..41873)
                     /gene="sohB"
                     /locus_tag="OO349_000917"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HCR5866088.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            42093..42851
                     /locus_tag="OO349_000918"
     CDS             42093..42851
                     /locus_tag="OO349_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HCR5866089.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            42848..43438
                     /gene="cobO"
                     /locus_tag="OO349_000919"
     CDS             42848..43438
                     /gene="cobO"
                     /locus_tag="OO349_000919"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HCR5866090.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIRHAFDAGVKAQIGIDY"
     gene            complement(43478..44350)
                     /gene="rluB"
                     /locus_tag="OO349_000920"
     CDS             complement(43478..44350)
                     /gene="rluB"
                     /locus_tag="OO349_000920"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HCR5866091.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGSRRSGGRNNG"
     gene            complement(44451..45071)
                     /locus_tag="OO349_000921"
     CDS             complement(44451..45071)
                     /locus_tag="OO349_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HCR5866092.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            complement(45068..45949)
                     /gene="yciV"
                     /locus_tag="OO349_000922"
     CDS             complement(45068..45949)
                     /gene="yciV"
                     /locus_tag="OO349_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HCR5866093.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQQIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            46087..46131
                     /gene="trpL"
                     /locus_tag="OO349_000923"
     CDS             46087..46131
                     /gene="trpL"
                     /locus_tag="OO349_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HCR5866094.1"
                     /translation="MKAIFVLKGWWRTS"
     misc_feature    46108..46201
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            46223..47785
                     /gene="trpE"
                     /locus_tag="OO349_000924"
     CDS             46223..47785
                     /gene="trpE"
                     /locus_tag="OO349_000924"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HCR5866095.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLIDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVESEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYIADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     gene            47785..49380
                     /gene="trpD"
                     /locus_tag="OO349_000925"
     CDS             47785..49380
                     /gene="trpD"
                     /locus_tag="OO349_000925"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HCR5866096.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAN
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGKIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            49381..50742
                     /gene="trpCF"
                     /locus_tag="OO349_000926"
     CDS             49381..50742
                     /gene="trpCF"
                     /locus_tag="OO349_000926"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HCR5866097.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLL
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            50754..51947
                     /gene="trpB"
                     /locus_tag="OO349_000927"
     CDS             50754..51947
                     /gene="trpB"
                     /locus_tag="OO349_000927"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HCR5866098.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            51947..52753
                     /gene="trpA"
                     /locus_tag="OO349_000928"
     CDS             51947..52753
                     /gene="trpA"
                     /locus_tag="OO349_000928"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HCR5866099.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            53134..53313
                     /locus_tag="OO349_000929"
     CDS             53134..53313
                     /locus_tag="OO349_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HCR5866100.1"
                     /translation="MAEHRGSSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            53399..53899
                     /locus_tag="OO349_000930"
     CDS             53399..53899
                     /locus_tag="OO349_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HCR5866101.1"
                     /translation="MNMKTIEDVFIQLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            53945..54451
                     /locus_tag="OO349_000931"
     CDS             53945..54451
                     /locus_tag="OO349_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HCR5866102.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSIGGYV
                     FEQFEITCYTSLLAAAKNAGDTVSIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(54511..55149)
                     /gene="ompW"
                     /locus_tag="OO349_000932"
     CDS             complement(54511..55149)
                     /gene="ompW"
                     /locus_tag="OO349_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HCR5866103.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
     gene            55389..55478
                     /locus_tag="OO349_000933"
                     /pseudo
     CDS             55389..55478
                     /locus_tag="OO349_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            55506..56249
                     /locus_tag="OO349_000934"
     CDS             55506..56249
                     /locus_tag="OO349_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HCR5866104.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSELIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            56279..56818
                     /locus_tag="OO349_000935"
     CDS             56279..56818
                     /locus_tag="OO349_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HCR5866105.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLSKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            56923..57321
                     /gene="yciA"
                     /locus_tag="OO349_000936"
     CDS             56923..57321
                     /gene="yciA"
                     /locus_tag="OO349_000936"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HCR5866106.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(57361..58089)
                     /gene="tonB"
                     /locus_tag="OO349_000937"
     CDS             complement(57361..58089)
                     /gene="tonB"
                     /locus_tag="OO349_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HCR5866107.1"
                     /translation="MTSITLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAP
                     AQPISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPK
                     PKPVKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALS
                     RNQPQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRY
                     EPGKPGSGIVVNILFKINGTTEIQ"
     gene            58304..58600
                     /locus_tag="OO349_000938"
     CDS             58304..58600
                     /locus_tag="OO349_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HCR5866108.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
BASE COUNT        14069 a        15155 c        14652 g        14725 t
ORIGIN      
        1 aatcagtaag ttggcagcat tacctactaa tgcaccttcc tgctgtaatg cctcgaccag
       61 cgctaatatt tcgctttctg gaagcaagcc acgcagtggg ttcaacccga tgccatcgtg
      121 agaggcgagg aagttaaacc aggtcgtttt actggagggc aagctcagac tttgcgccca
      181 ctggcacagc gcctcgacgt tctgtttttg caccgcatgt aacaccagcg gcggcagcga
      241 gaactggtac accatatgcg cttcgtcatc accgttgcca aagtaagcaa tgttgtcttt
      301 gtgcggaaca ttggtttcgg taatgatcac tgtacctggt gctacgtcat caataatcga
      361 ccgtaacagc ttgataatca gatgtgtttt ttccagatgg atgcagcttg ttcccggctc
      421 tttccacata aagccaacgg catccaggcg gacatactca gcacctttct caaggtaaca
      481 gagtaaaaca tccaccatcg ccagcaacac ttcagggcta cggtaattca ggtcaatttg
      541 atcgtcacta aaggtggtcc ataaatggcg cgtagaatta tcatccattt gaaacggcgt
      601 taatagcggt aacgcacgcg gaagggtgac ggcactgaga tcggttttcg gatcaaccgc
      661 aataaagaaa tcttcaaaac ccggaagctg ttgtaaatag tttttaaacc attcactttt
      721 tgcagacata tggttgcaga caaaatcaaa cattaaatga ctgcattcac cgagttgctg
      781 aatatcctgc cactcccccg cttcactggc gacctgatga taatcaatta ccgaaaatcc
      841 atcatcagat gaccatgggt aaaaaggtaa taaatgaaca tgtgaaaaaa tgctttgcag
      901 ccattgatgg tagaactgat taaatatggg tagtggtttt aaatcattac tgtgaaattg
      961 atcggcatag gtaattaaca cgacatcact ttcatcccag tgttttttac gtcgctgtgt
     1021 aattaatcgt ttcgcactct caagacgcgt tacaagtttc tgtaaatgag ttgcggtaaa
     1081 tgttccaccg tagatttcgt ccatgtaatc cgtaattttc tgtttcatgt tgcctttccg
     1141 tggtagcggt cccatgaggc gagagactac tccgcagaaa ataaactgtc aattgagcac
     1201 aagggtttta gtcacgcagg aaatcatttt ttaccaggga aaaagcgtat ttgtgagctg
     1261 acgcaaataa tctataggga tgtaaataag gtgtgacaga aaaaaaacgg cgcataagcg
     1321 ccgctcatgg tgaattcttt taacctttga ccttcggcat tgcgatgtct ttcaggccac
     1381 cggcgttctc aacgtgggta tatcccatct cgctaaggag ctcttttgcc tgcccggact
     1441 ggcgtccggc attgcaatac actttcacgg tgtcgttttt atccggaacg gcggtggcaa
     1501 tgcgctcttt cacttccttc aggggaatat tgatggcccc ctgaacgtgc tcttgctgat
     1561 actgctctgg aacacgaaca tcgatccagt gttcagcggc gaaaacgggc agtgaaaaca
     1621 ccagtgccag agcaagtaag ccttttttta aacatgtaag cgtccttcta tggatgaaaa
     1681 ttgccatcca ttatacgcct gagatttaac aatttttgcg aaactcccct caccttttat
     1741 aacgctgtgc cagtttatta gcagcccgac ttaacagcgg ttccagcgcc acagccagca
     1801 acattttcag cggtcggcga gcaactgatt ttatcgccca gcccgccaca cccgccgggc
     1861 catagcgcag tgcggtaaga agtaccagct tgcctgctaa tttgaaacca agcttcacct
     1921 tttgcccggc ctgttgccag cgagtattca taactttcct cacagttgac ggaaacggct
     1981 acgtaacgtg aaagtatcgg aagtgacata acgttccatc tcgcgtaaac gcgtttcact
     2041 tgccgccagt tcacggtcga cttcatccag caattcgctg ctggaaggta gctgctcacc
     2101 aaaggccatg ttgtccggca ttggatcaag cgcaaatgac aaaatgatgt aagcaaccag
     2161 ggtaaacagc gccagaccga agaaaatcga cagcaccacc aggatacgca ccagttttac
     2221 cggtacatca aaatagttgg caatcccggc gcagacgccg cggaccatgc cctgctgtgg
     2281 aatacgccat aactttttat tgagattaat gcccgccatt agcgatccct ccagttcgga
     2341 tgttctgcat caagaataga ttccagcgcc tgaatacgtt cgcgcatccg ttttgcttca
     2401 tcaaccagtt gcgctaatcg ctgctgctca ctttgcgaca attcactgcg accagaacga
     2461 ttgctgtaat gcagccataa ccagatcggt aaaacaaaca gcacaaaaat ggttaacgga
     2521 atagccagaa atagcgcgct cataagtact ccttagacga tgagcggcgg cgcgttcgga
     2581 cgccgcctgg atgttattat tgattgtctt gcttcatttt ggcttttaat tgtgccagtt
     2641 gttcgctgat tgcatcatcg gctttcagtt cggcaaactg atcgtccagc gatttttgtt
     2701 taccgaagct gtggctttct gcttccgctt ccatctggtc aatacgacgt tcgaaagatt
     2761 cgaaacgagc cattgcttca tccagtttgc cactgtccag ctgacgacgc acatcgcgcg
     2821 acgagtttgc cgcctgatga cgtaacatca atgcctgctg gcgagcgcgt gtttcgctca
     2881 atttgttttc cagctcgcca atctctttct tcatgcgtgc cagcgtatcg tccaccagcg
     2941 tcacttcatg ttccagggac ttaatcagat cggtcagttt ctgtttttca attaacgctg
     3001 cacgtgccag atcctctctc tctttcagca gcgccagttc ggctttttcc tgccattcaa
     3061 cttcacgcgc cgacgcttgt tcaatacggc gagtcagctg tttcttttct gccaacgcac
     3121 gcgccgaagt agaacgtact tcaaccagtg tatcttccat ctcctggatc atcagacgca
     3181 ccagtttctg tggatcttcc gctttctcta acagagcgtt gatgttggca ttcacgatgt
     3241 cggcaaagcg agaaaaaata cccataatgt tgtcctcttg atttctgcgt tcaggattgt
     3301 cctgctgaac tgttaataca atttgcgtgc caatttttta tctttttgat ttataaagat
     3361 ctgattgaag aatcaacagc aacatgccag gatgaattag cgaattacac taacaagtgg
     3421 cgaatttcat catggccgaa tacaaagata atttacttgg tgaggcgaac agctttctcg
     3481 aagtgctgga acaggtttcg catctcgcac cgctggacaa accggtgctc atcatcggcg
     3541 aacgcggcac cggtaaagag ttgattgcca gccgcctgca ttatctctcc tcccgttggc
     3601 aagggccgtt tatttccctt aactgcgcgg cgttaaatga aaatctgctg gattccgaac
     3661 tgtttggtca cgaagcgggg gcgtttaccg gtgcgcaaaa acgtcatcct gggagatttg
     3721 aacgtgccga cggcggtacg ctatttcttg atgaactcgc tacggcaccg atgatggtgc
     3781 aggaaaaatt attgcgtgtg attgagtacg gtgaactgga gcgcgttggc ggtagccagc
     3841 cgttgcaggt gaatgtgcgg ttggtatgcg cgacgaatgc cgatctcccg gcgatggtca
     3901 atgaaggtac ttttcgcgct gacctgctcg accggctggc tttcgatgta gtgcaactgc
     3961 cgcccctgcg cgagcgcgaa agcgacatta tgctgatggc agaacacttt gccattcaga
     4021 tgtgtcggga aatcaagctg cctctgttcc cggggtttac ggagcgcgcc agagaaacat
     4081 tgctgaatta tcgttggccg ggaaatattc gtgaattgaa aaacgtggtg gaacgttcag
     4141 tgtatcgcca cggcaccagc gattatccgc ttgatgacat cattattgat ccctttaaac
     4201 ggcgtccacc tgaagaagcc atcgccgttt cagaaaatac ctcgcttccg acgctgccgc
     4261 tggatttacg cgagtttcag atgcagcagg aaaaagagtt gctgcaactc agtttgcaac
     4321 aggggaaata taaccagaag cgcgcggctg aattactggg gttaacttat catcagttcc
     4381 gcgcgttgtt gaaaaagcac cagatttagc gaagttgagc aatacaactt gccggatgcg
     4441 gcgtgaatgc tttattcggc atacataatc aatacgtcgt ttgcaggcct gataagcgca
     4501 gcgcatcaga cattattgcg ttgggctatt aatcgctcag cgcatcctgc aaaattttca
     4561 ttgccgcatc gaattgcgca tccgggatgg tcagcggata caggaagcga atcacgttgc
     4621 cgtatgcgcc acaggtcagc aggagcagcc cctgcgccag cgcgcgttgc tggattttct
     4681 gtgcaatcgc cgctgacggc tcgcccgttt gcggatcgtt aaactctgcc gcaatcgtcg
     4741 accccaggcc gcgtaccgca gcaatggccg gaacgctttc tttggcatca atcaacgtgt
     4801 ttttgagacg ctggcccagt tgattcgcgc gttcgcagag tgattctttg tcgataatat
     4861 tgagcaccgc gtgcgcggca gccaccgcca gcgggttccc ggcgtaggtg ccacccaacc
     4921 cgcccggcgc gggcgcgtcc ataatattcg cgttaccgac cacgcccgaa agcggcatcc
     4981 cgcccgcgag gcttttcgcc atcgtcatta agtccggctt atcggcgtaa tgatccatgg
     5041 caaacagttt accggtacgc gcaaagccgc tttgcacttc atcggcaatc atcacaatac
     5101 cgtgctcgtc gcacaggcgg cgaatggcgg caaccagctc ttttggcgca acgttgaaac
     5161 cgcactcgcc ctgcaccggt tcgaaaataa tggccgcgac ctgcttcgct tcgatgtctg
     5221 atttaaacaa gcgttcgatg gcgtcgaggg agtcctgtgt tgaaatgccg tgtaaatctg
     5281 acggataagg tacgtgatac accgaaccag ggaacgggcc gaagccgatt ttgtacggcg
     5341 caacttttcc ggtcagcgcc atggtcatat atgtacgacc gtggaagcca ccgctaaacg
     5401 caatcacgcc aggtcgtccg gtatgggcgc gagcaatttt caccgcgttt tccaccgctt
     5461 ccgcgccggt agtgaagaat gcggttttag cctgcccgct cactggggca agggtgttaa
     5521 ttttctccgc cagcgtgacg tagctttcgt acggcacaat ctgatacgcc gtgtgggtaa
     5581 actgttgcaa ttgctgctcc accgccgcga ccagatcagg gtggcgatgc ccggtattca
     5641 gcaccgcaat gcctgcggcg aaatcgatgt attcgttgcc ctctacgtcc ttcagcgtag
     5701 cgttttctgc cgactgggcg aagaagttac acatcacgcc aaccccgcgt ggggtagcag
     5761 aaagacgacg ctgatggaat tcattgttgc tcatgtttca caccttttga ctcttcactg
     5821 ttaatcggaa attaaacgcc cagacgatcg cgcaggctgt aataagccgc gcccatcgcg
     5881 gtaaatggca cgcgcagcgt gcgcccgccg gggaacgggt aatgcggcag attggcgaag
     5941 gcatcgaaac gctcggcgtc accgcgcagc agttcagcaa tcaaacgccc ggcaagatgg
     6001 gtacaggtga cgccgtggcc gctgtaaccc tgcatgtaat agatgttggt atcaaggcgg
     6061 ccgaactgcg gcatgtgcga caaggtcagc aggaagttgc ccgtccagcg gtaatcaatt
     6121 ttcacgccct tcaactgagg gaaggttttt agtagtttcg gtaccacaag acgctcaacg
     6181 tcatccgggt tgcgtgcacc atacactaca cccccgccgt acagcaggcg gttgtcggcg
     6241 gtaagacggt agtaatcaag cagatagtta cagtcttcca cacagtagtt tttcgggatc
     6301 agcgaacggg ctaaatcttc cgacagccgt tcggtggtga tcacctgcgt gccgcacggc
     6361 atgctgcgtt tcgctagttc cggctctact ttatcgccca gatacgcatt cccggcgaca
     6421 atcacatact tcgccgtcac ctgaccattg gcagtccgaa ccacggctgg cgtggtgtgc
     6481 tggatctgcg tcacggcgga aagctcatac acgcgcccgc cgttcaggcg gatggcatcg
     6541 gcttcaccaa tggcgaggtt tagcgggtga atatgcccac cgctgtgatc cagcagcgca
     6601 ccagtgtagc gatcgctggc gacctcgcgg cgaatagcgt tgacgtccag cagctccagc
     6661 tgtttattgc cgtagcgttc ccagttctct ttctgctctt cgagtgtggc gagttgttta
     6721 tcattcatcg ccacaaacag accgccgggg cggtagtcgc agtcaatctg ataacgtttg
     6781 atacgttcgc ggatgatctc gccaccctcg aacatcatgc tgccgagcat acgggcggtg
     6841 tccatgccgt agcttttttc gatcacgtcg atgtcgcggc tgtaggagtt cacaagctgc
     6901 ccgccattgc gcccgcttgc gccaaagccg atgcgtgagg cttcgagaac cactacgtca
     6961 aagcccgctt ccgccagatg cagcgcggag gagagcccgg tatagccgcc gccaaccacg
     7021 caaacgtcgc aggtgattga ctcattcagt gtgtcgaatg gtgcatattt attcgcactg
     7081 gcggcgtagt aactgctggt atgttcggtc atttcaggcc tccaggctta tccagatggt
     7141 tttcagttca gtgaattttt caagggcatg cagggatttg tcgcgaccgt tgccgctctg
     7201 cttatagccg ccaaacggca cggtcatatc gccgtcgttg tagttattga cgaagacgga
     7261 accggctttc aggcgtcggc tcatgcggtg cgcgcgggag aggtcgcgcg tccataccgc
     7321 cgcgccaagg ccgtactggc tgtcgttggc aagctgcagc gcctgatctt ctgatgtgaa
     7381 acgggtgacc accagcaccg gaccgaaaat ctcttcgcgg cttaaggacg cattcgggtc
     7441 cacatccacg aagatggtcg ggccgatggc cgctgccagc ccggcgttgc gaccatccag
     7501 cagcagttgc cccttgcctt cgccttcacg aataaaggtg tgtaccgagt cagcgtgagc
     7561 gctgtcgatt aaggtgccca tggtggttgc gggatcaagt ggatggcccg gctgccagtt
     7621 ttgcgcctgc tgttttaaca gggctaagaa ttcatcggcg atgctctctt ccagtaacaa
     7681 acgcgttccg gcgatgcaca cctggccctg gttgtagaag atgcctgcgg cggtggcgct
     7741 tgccgctttt tgcagatccg gacaatcggc gaaaacgatg ttggcgcttt tgccgcccgc
     7801 ttccagccag acacgtttca tgttgctatc acccgcgtct ttcagcagtt gtttacccgt
     7861 acgcgtggag ccggtaaagg cgatggcatc gatatcgtta tgacgcgaaa gcgcctgccc
     7921 cgcttcatga ccaaaacccg tcaccacgtt caacacacca tccggcaagc ctgcttcttt
     7981 cgccagcccc gcgagacgaa tcgcactgag cggtgatttt tcagacggtt ttagaatcac
     8041 gctgtttccc gccgccagcg ccgggccgag tttccagcaa gtcagcaaca gcgggaagtt
     8101 ccacggcacg atggcggcaa tcacgccgac cggttcacgc acgatcatcg ccagctcatg
     8161 gctactggtg gtcgccactt cgccatacac tttgtcgatc gcttcggcgt accagcgaat
     8221 ggcgcgcgcc gcgccgggaa tatcatcacg cagactgtga cgaatcggtt tgccggtgtc
     8281 gagagtttcc agcagtgcca gctcttcggc gtgggcttcc attaaatcgg cgagtttatt
     8341 cagtaccgct ttacgtttag ccggagaaga gagtgaccag tcgccgcgtt caaatacgcc
     8401 gcgtgctgcg ctcaccgcac ggtcgatatc gacgctcttg ccgcgggcaa ttttcgccag
     8461 cggtgcctgg gtgaccggat caacggtttc aaaggtttca ttttccgccg cagcagtata
     8521 ttcaccgtta ataaataagc ggttttcaat ggcgagactt aacgctttat cctgccagta
     8581 agccagatga tgaaaattca ttatgactcc tgtttcacgt ctatcagata tatgcagttt
     8641 gtgaacgtca gggcgcagag atccccgggc tgcatgcccg tttatttctg gcgacattct
     8701 ttatggattg ccgggcgcat tgcgcttacc cggcttacag aacaaaaaga ttaaaacgtg
     8761 gtgggcgtat gggcgctgat aattcggcaa atacctgccg acgtattact gaaactgtgc
     8821 gggatgccgg tattaatggc ataactttgc cccgcgacga ggtggtaatc ctgaccatta
     8881 atcgtcagaa caatttcacc ttccagtact gtgcctattt cctcaccctg atgcttaatt
     8941 ctttccccag tggttgtgcc cggctggtac gtttcaaaga tcatcgccag cgtgcgattc
     9001 gggttaccgt tatgaaccag cttcattgac acaccctgac tgcccatctc aattaagtcg
     9061 tcctgattaa tgacgacctg cggctcatca ggtttttccg gctcggaaaa gaattccgag
     9121 agtgacaaac catacacctt cagcagcttt tgcagcgtac tgatggcagg gctgacttta
     9181 tcttgttcta tcgtactgat agcactgtga gtcagcccgg agagttcggc ggcacgacgt
     9241 tgtgaaagcc cctgctgctg gcggatttcc gacaagcgtt ttcctggcgc cagtccctca
     9301 tcactcatat ttgcatttcc ttaaactgta gtggtcagag tcgctgtttt tcagcgatat
     9361 ggtgctgcca agcggtgata aagccctcga acaatatacg cgaaagcgcg tactcgctac
     9421 tgttccattc cgggtgccac tgtacgccca gcgcaaaagg atgattgatg acgctaaccg
     9481 cctcaactaa gccatccggc gagcgagctt caacacgcaa ccgtgggcta acgaccttcg
     9541 ccccttgtcc atgtagagag tttacccaaa agttgctaca ttcaggtaac aacgcagaca
     9601 gtaatccccc ctcttcaacc tgaacttcat gcgacggtgc atattgctgt tccaccggca
     9661 gttcgggatc ttcccgatgt tccagcaatt caggctgttc gcacagcttg cgatgcaacg
     9721 aaccaccggt tgccaccacc agttcttgta aaccccggca gatggcgaaa atggggatgc
     9781 gcctttcgag tgcggcatta attatcgcca tgctcagaag atcacgcccg ggatcggcgt
     9841 caggctcatc gccgttttca ccatataggt gcggctgcac attgctggga ctactaggaa
     9901 gataaatgcc atcgagtttc ggcaaaagtt gttcaagtaa cgacggttcc gccagcgcat
     9961 gtggtagcgc aattggcaag ccgcctgcgt ggatgatggc attcaggtac ttttcttgca
    10021 gagtctgggt cgcatgaccc ttaagcctgt tcctgcacat tacgacaccg ataaccggat
    10081 tgttcattat attttccatg ctcaatctca caaagtggac taaattatcg ccattactgc
    10141 gctttaaatg acctttatgt tcaatatttt ttcaatctag cagtggattt tcatttttgc
    10201 aaactcaatt taacatttga caaacatttg gtttgcatac agattcgaat gatggtcatt
    10261 atattttacg ctttgataac gagcggaaaa caaaccaaag gcgaagaatc atggaaacca
    10321 atatcgttga agtagagaac tttgttcagc agtcagaaga gaggcggggt agcaccttta
    10381 cgcaggaagt gaaacgctac ctggagcgct acccgaatac gcaatatgtt gatgttttac
    10441 ttactgattt gaatggttgc ttccgtggaa aacgtatccc tgtttcaagc ctgaagaagc
    10501 tcgagaaagg gtgttatttc ccggcctcgg tatttgcaat ggatattctg ggcaacgtag
    10561 tagaagaagc aggtctgggt caggaaatgg gcgagccgga tcgtacctgt gttcctgttc
    10621 tcggttcctt aactccttct gccgccgatc cagagtttat cggtcagatg ctcctgacca
    10681 tggtcgatga agatggcgct ccctttgacg ttgagccgcg gaacgttctt aaccgcctgt
    10741 ggcagcagct gcgccagcgc ggattgttcc cggttgtagc ggtagagctg gagttctatt
    10801 tactggatcg ccagcgcgac gctgaagggg atctgcaacc gccctgcgcg cctggcaccg
    10861 atgaccgcaa tacacaaagc caggtttact ccgttgataa cctcaaccac ttcgccgacg
    10921 tgctcaatga tattgatgaa ctggcgcagt tgcagctgat tccggcagat ggcgcggtcg
    10981 ctgaggcctc gccgggtcag tttgaaatca acctttacca tactgataac gtgctggaag
    11041 cctgcgatga tgcgctggca ctaaaacgcc tagtgcgtct gatggcagaa aagcataaga
    11101 tgcacgccac ttttatggcg aagccgtatg aagagcacgc gggcagcggg atgcatatcc
    11161 atatcagtat gcaaaataat cgtggcgaga atgtgctttc tgacacagaa ggcgaagatt
    11221 cgccgctgct gaaaaagatg ctcgccggga tgattgacct gatgccgtcg tcgatggcgt
    11281 tgctggcacc aaacgtgaac tcgtatcgcc gcttccagcc gggaatgtat gtgccgacgc
    11341 aggcgtcgtg gggccataac aaccgcaccg tcgccctgcg tattccgtgc ggtgaccgtc
    11401 ataatcaccg cgtggaatat cacgtggcgg gtgccgatgc caacccatat ctggtgatgg
    11461 cagcgatttt tgccggtatt ttgcatggcc ttgataacga gctgccgttg caggaagaag
    11521 tcgaaggcaa cgggctggaa caggaaggct tacccttccc gattcgccag agcgatgccc
    11581 tgggtgagtt tatcgagaat gatcacctgc gccgctattt aggcgaacgg ttttgccatg
    11641 tgtatcacgc ctgtaaaaat gatgagctgt tgcagtttga gcgcctcatc acagaaacag
    11701 aaattgagtg gatgttgaaa aacgcgtaat tccccttgcg gggttgtttc atcggcctga
    11761 gcggagccag gcctgacatt tgctggtaat ccagacattt cccggatgtc gcgttatgga
    11821 gcgcaggcgg caacgggctt ccgttaacga ttaagactgt gacgaggaaa tgagatgatg
    11881 ccgatgatta aatccccgca tggcgaggga ggctgcgtat gcgcccctcc tgctactgac
    11941 tggactccgc cccctttgct tcccttgcta aacaggtttg atttcaggtc gacacgaccg
    12001 caaaccttat tacgcagggg aggcagcaat tatggctatt aattcaccac tgaatattgc
    12061 tgcgcaaccc ggcaaaaccc gtctgcgaaa atcactgaaa ttgtggcagg tggtaatgat
    12121 gggtctggcc tatctcacgc cgatgaccgt atttgatact tttggtattg tctccggcat
    12181 tagcgacggt cacgttccgg cgtcctattt gctggcgctg gcgggcgtgc tgtttaccgc
    12241 tatcagctac ggcaaactgg ttcgccagtt tccggaggcc ggttcggcct atacctacgc
    12301 gcaaaagtcg attaacccgc acgtcggatt tatggtcggc tggtcatcac tgctggatta
    12361 tctctttttg ccaatgatca acgttttgtt ggcgaaaatc tatctctccg ccctcttccc
    12421 ggaagtgccg ccgtgggtgt gggtggtaac cttcgtcgcc attttaaccg ccgcgaatct
    12481 gaagagcgtc aacctggtcg ctaacttcaa taccctgttt gtactggtgc aaatctccat
    12541 catggtggtg tttatcttcc tggtggttca gggactgcat aaaggagaag gcgttggcac
    12601 cgtctggtca cttcagccgt ttatcagcga gaacgcgcac ctgatcccga ttattaccgg
    12661 tgcgacgatt gtctgtttct cgttcctcgg tttcgatgcg gtgaccacgc tttcggaaga
    12721 gacgccggac gccgcacgcg tgatcccgaa agccatcttc ctgacggcgg tatacggcgg
    12781 cgttatcttt atcgcggcgt cgttctttat gcagctgttc tttcccgata tcagccgctt
    12841 taaagacccg gacgccgcac tgcctgaaat tgcgctgtac gtcggcggca agctctttca
    12901 gtcgattttc ctctgcacca cgtttgtgaa cacattagcg tctggcctgg cttcacatgc
    12961 cagcgtgtcg cgtctgctgt atgtgatggg gcgtgacaat gtgtttccgg agcgcgtgtt
    13021 tggctatgtg cacccaaaat ggcggactcc ggcactcaac gtcattatgg tcgggattgt
    13081 cgcgctgtcg gcgctgttct ttgatttagt caccgcgaca gcactgatta acttcggtgc
    13141 tctggtggcg tttaccttcg tgaatctgtc ggtgtttaat catttctggc ggcgtaaagg
    13201 aatgaataaa agctggaagg atcacttcca ctatttgctg atgccgctgg ttggcgcgct
    13261 gacggtgggt gtgctgtggg ttaacctaga gtcaacgtca ctgacactcg gtctggtatg
    13321 ggcttcgtta ggcggcgcat atttgtggta tctgatccgc cgctatcgca aagtgccgct
    13381 gtacgaaggt gacagaacgc cggtgagcga aacgtaatag ccggatgcgc ggcgcattat
    13441 cctgcctgag ggtaacgcgc cgcgccatta actataaaaa gatgtatgac ccggatcacg
    13501 agtgctatca tccgccctta tgcttagcgc attaacccca ggggcaaata atgaaccacg
    13561 atatcccgct aaaatatttt gatattgccg atgagtacgc gaccgaatgc gcagagcctg
    13621 tcgcagaggc tgaacgcaca ccgctggccc actacttcca gttactgctc acccgtttga
    13681 tgaacaatga agagatcagc gaagaagccc aacatgaaat ggctgctgaa gcggggatta
    13741 atcccgtacg cattgatgag atcgcagagt ttttgaatca atggggtaat gagtaattat
    13801 ttaattgcag acgatcggtc agtatcgttg tccggataaa gcgttcacgc cgcatccggc
    13861 taacaatgac acgcactgca gcgtgcttta atgggtttct cacgatcgta ccgctctgct
    13921 cctcgctaag ttgttctgtg agttatagtt atagagaaat cacgggtacg aatgttgaac
    13981 agatggcgac gccacctgtt caacgaccaa ccacacggcc ctcattacga cccctgaaca
    14041 caaacggtaa tggcaaatgt cattaaatat tagtaagata gcctctttac ataaaatccc
    14101 ttaatattat gcgccaggta ttatcgtctc ttttggtgat tgctggactt gtgagtggtc
    14161 aggcaatcgc cgcgcctgaa tcccccccgc atgctgatat ccgcgacagc ggttttgtct
    14221 attgcgtcag cgggcaagtc aacaccttta acccatccaa agcgagcagt gggttaattg
    14281 tcgataccct tgccgcccag ttttatgatc gactgctgga tgtcgatccc tatacctatc
    14341 gcctgatgcc tgaacttgcc gaaagctggg aagtactcga caacggcgcg acctatcgct
    14401 tccacctgcg tcgcgatgtt ccgtttcaaa aaaccgactg gtttactccc actcgtaaaa
    14461 tgaatgccga cgatgtggtg tttaccttcc agcgaatttt tgaccgcaac aacccgtggc
    14521 ataacgtcaa cggcagcaac ttcccctact tcgacagcct gcaatttgcc gataacgtga
    14581 aaagcgtccg caaactggat aatcataccg ttgagttccg actcgcccag ccggatgctt
    14641 cttttttgtg gcacctcgca acccattatg cttcggtcat gtcggcagaa tatgcccgga
    14701 agttagagaa agaagatcgc caggagcaac tcgaccgtca accggtcggc accggtccgt
    14761 atcagttgtc ggaataccgc gccgggcaat ttattcgcct acaacgtcat gatgacttct
    14821 ggcgcggtaa accgttaatg ccgcaggtag tggtggattt aggctccggc ggcaccggac
    14881 gtctgtcgaa actcctgacc ggggaatgcg acgttctggc ctggcctgct gccagccagc
    14941 tatccatttt gcgtgacgac ccgcgcttgc gtttaacgct gcgtcctggg atgaacgtcg
    15001 cctatctggc atttaacacc gccaaaccgc cgctaaataa tcccgctgtc cgccatgcac
    15061 tggcactggc gattaataac cagcgcctga tgcaatccat ctattatggt acggctgaaa
    15121 cggcggcctc tattttaccg cgcgcctcgt gggcctatga caacgaggct aaaattactg
    15181 aatacaatcc ggcgaaatcg cgcgaacagt tgaaggcgtt ggggctggaa aacttaacgc
    15241 tgaaactgtg ggtgcccaca cgttcgcagg cgtggaaccc cagtccactg aaaactgccg
    15301 aactgattca ggcggatatg gcgcaggttg gcgtaaaagt ggtgattgtg ccggtagaag
    15361 gtcgctttca ggaggcgcgg ttgatggata tgagccatga tctgacgtta tccggttggg
    15421 cgacggacag taacgacccg gacagtttct tccgtccgtt actgagctgc gcggcaattc
    15481 attcacagac caacctcgcc cactggtgcg atccgaaatt cgacagcgtg ttgcgtaagg
    15541 cgctctcctc gcagcagctg gcagcgcgta ttgaagccta tgacgaagcg cagagtattc
    15601 tggcgcagga attacctatt ctgccgctgg cgtcgtcatt gcgtttgcag gcctatcggt
    15661 acgatatcaa aggtctggta cttagcccgt ttggtaacgc ctcctttgct ggggtgtatc
    15721 gcgagaaaca ggatgaggtg aaaaaaccat gattatcttc accttacgcc gcattttgtt
    15781 attgattgtc accttgttcc tgctgacctt cgttggcttc agcttgagct atttcactcc
    15841 acacgcgccg ttacaaggcg cgtcactgtg gaatgcctgg gtgttctggt ttaatggcct
    15901 gatccactgg gattttggtg tttccagcat taacggtcaa ccaattgccg aacagttaaa
    15961 agagctcttc ccggcgacga tagaactgtg catcctcgcc ttcggttttg cgctgattgt
    16021 tggcatcccc gtggggatga ttgccgggat tacgcgccat aagtggcagg ataacttgat
    16081 caacgccatc gccttactgg gcttttcaat cccggtgttc tggctggcgc ttctgttgac
    16141 actgttttgt tcactcacgc taggttggtt gcccgtttca gggcgtttcg atctgctcta
    16201 cgaagtgaaa ccgattaccg gttttgcgtt gattgatgcc tggctttcgg actcaccatg
    16261 gcgggatgaa atgatcatga gcgcaatccg ccatatgata ttgcccgtga tcaccctgtc
    16321 ggttgcgcca acaactgaag tgatccgcct gatgcgtatc agcactattg aagtgtatga
    16381 ccagaactat gtaaaagcgg cggcgacccg cggtttgtcg cgctttacta ttttgcgtcg
    16441 ccacgttctg cataacgcgt taccgccggt tattcctcgc ctgggcttac agttttccac
    16501 catgctgacg ctggcgatga tcaccgaaat ggtttttagc tggccgggtt tagggcgctg
    16561 gttaattaac gccatccgcc agcaggatta cgcagccatt tccgccggag tgatggtgtg
    16621 tggctcactg gtcattattg ttaacgtgat ttctgatatt ttgggtgcca tggctaaccc
    16681 tctgaaacat aaggaatggt atgccttacg atagcgtata cagcgaaaag cgcccgccgg
    16741 gcacgctgcg taccgcctgg cgcaaatttt atagtgacgc ctctgcgatg gtcggcctgt
    16801 acggctgcgc gggactggct gtactgtgta tttttggcgg ctggtttgcg ccttacggca
    16861 tcgaccagca atttctcggt tatcaattat tgccgccatc atggtcgcgc tatggcgaag
    16921 tttctttctt cctggggact gacgacctcg gacgtgatgt tttaagccgt ttactgagcg
    16981 gagctgcgcc aaccgtgggt ggcgcatttg tggtgacgct tgccgcgacg atctgtggcc
    17041 tggtactggg caccttcgcc ggggcgacac acggcctccg ctcagcggtg cttaaccata
    17101 ttctggatac cttactggcg atcccttcgc tgctgttggc aattatcgtt gtagcgtttg
    17161 ccggaccgag tttgtcccac gctatgtttg ctgtctggct ggcattgctg ccgcgtatgg
    17221 tacgttcgat ttacagcatg gtgcatgacg aactggaaaa agagtacgtt atcgccgccc
    17281 gtctggatgg cgcatcaacg ctgaatattc tctggtttgc tgtgatgcca aacatcaccg
    17341 ccgggctggt caccgagatc acccgcgcac tgtcgatggc aattctcgat atcgccgcgc
    17401 tgggctttct cgatctcggc gcacaactcc cctcgcctga atggggagcg atgctcggtg
    17461 atgcgctgga actgatttat gtcgccccgt ggactgtcat gctgccaggt gcggcaatta
    17521 tgattagcgt gttgttggtt aacctccttg gcgatggcgt tcgtcgtgcg attattgcgg
    17581 gggtggaata atgccattac tcgatattcg taacctgacc attgaattta aaaccggtga
    17641 tgagtgggtt aaagccgtcg accgggtaag tatgacctta accgaaggtg aaatccgcgg
    17701 tcttgttggt gaatccggtt ccggcaagag tttgattgcg aaagcgattt gtggggtgaa
    17761 taaagataac tggcgtgtta ctgctgaccg tatgcgtttt gatgatatcg atttgctgcg
    17821 tctctccgca cgcgaacggc gcaaactggt tggccataac gtgtcgatga ttttccagga
    17881 accgcagtcg tgtcttgacc cttcagaacg tgtgggtcgc cagttgatgc aaaacatccc
    17941 agcctggacc tataaaggcc gttggtggca gcgttttggc tggcgcaaac gccgtgccat
    18001 tgaactgctg caccgcgtgg ggattaaaga tcacaaagat gcgatgcgca gttttcccta
    18061 tgagttgacc gaaggtgaat gtcagaaagt gatgatagcc attgcactgg cgaatcaacc
    18121 gcgtctgctg attgctgacg aaccgacaaa ctcaatggag ccaacaaccc aggcgcaaat
    18181 ctttcgcctg ctgacgcgtc tcaaccaaaa cagcaatacc actattttgc ttatcagcca
    18241 tgacttacaa atgcttagcc aatgggcgga taaaattaac gtgctttact gcggtcaaac
    18301 agtggaaacc gcgccgagta aggagttggt gacgatgcca catcatcctt atacccaggc
    18361 gctgatccgc gcgataccag acttcggcag cgcgatgccg cataaaagtc gcctcaatac
    18421 tctacccggc gctatcccat tgctggaaca gttaccgatt ggttgtcgtc tggggccacg
    18481 ttgcccgtat gcacaacgag aatgcattgt gacgccacgt ttgacggggg cgaaaaatca
    18541 tctctatgcc tgtcatttcc cgctgaacat ggagaaaaag tgagatgatc gaaacgctgc
    18601 ttgaagtgcg taatttaagt aaaacgttcc gctaccggac cggttggttt cgtcgtcaga
    18661 ccgtagaagc ggtaaaaccc ttgagcttta cgctacgtga aggccagaca ctggcgatta
    18721 ttggcgagaa tggttcgggt aaatccacac tggcaaaaat gctggcggga atgatagaac
    18781 ctaccagcgg cgagttattg attgacgatc atccgctgca ttttggcgat tattccttcc
    18841 gtagtcagcg tattcgcatg atttttcagg atccttcgac ctcgttgaat ccccgtcaac
    18901 gtatctcaca aattctggat tttccactgc gcctgaacac cgacctggaa ccggaacagc
    18961 gtcgcaaaca gattattgaa acgatgcgta tggttgggct gctaccggat cacgtcagtt
    19021 actatccgca tatgttggca cccggacaaa aacagcgtct gggtctggcg cgcgcgctga
    19081 tattgcgccc aaaagtcatt attgccgatg aagccctcgc ttcactggat atgtcaatgc
    19141 gttcgcagtt gattaatctg atgctggagt tacaggaaaa acagggcatt tcgtatattt
    19201 atgttaccca gcatatcgga atgatgaagc acattagcga ccaggtgctg gtgatgcatc
    19261 agggcgaggt tgtcgagcga ggcagcaccg cggatgtgct ggcatcgccg ctgcatgaac
    19321 tcaccaaacg gctgattgcc gggcactttg gcgaagcatt gacagcggat gcgtggcgta
    19381 aagatcgcta attttctgaa tcctccttcc agattattca cctcattctg acaagagatt
    19441 aaatcttgtc agaatgaggt gatggataaa ataaaatcaa atgcgcgtga tttaagacgc
    19501 aatctcactt tgcaggaacg aaagctctgg cgatatcttc gcagccgacg ttttggtgat
    19561 ttcaaatttc gccgtcaaca tccggtgggg agctacattc tcgattttgc ttgctgctcg
    19621 gcgcgcgtag tcgttgagct ggatggtggg cagcatgatt tggcagttgc ctatgatacc
    19681 aggcgcacta gctggcttga gtcgcagggc tggaccgtgc tgcgtttctg gaataacgag
    19741 attgattgta atgaggaggc ggtgctggag attattctgc aggaactgaa ccgccggtca
    19801 ccctcaccct gaaagggcga gggggccgac cgagccgaat agctgttgtg gtgaaaacat
    19861 ggagacagtg ctggagaata ttcggcaagg tctgaaccat cccagccatc gccataaaag
    19921 ggttaggggc tgtatgagcc tgtttgttgc tggggtaaca atatttgcac aatatggtcc
    19981 cctcgcccct ctggggagag ggttagggta aggggaaaag cgccccccct gccgcagcct
    20041 gctccggtcg gacctggcaa ctatagctac tcacagccag gttgattata ataaccgttt
    20101 atctgttcgt actgtttact aaaacgacga atcgcctgat tttcaggcac aacaagcatc
    20161 aacaataagg attaaagcta tgggttttct ttccggtaag cgcattctgg taaccggtgt
    20221 tgccagcaaa ctgtccatcg cctacggtat cgctcaggcg atgcaccgcg aaggagctga
    20281 actggcattc acctaccaga acgacaaact gaaaggccgc gtagaagaat ttgccgctca
    20341 attgggttct gacatcgttc tgcagtgcga tgttgcagaa gatgccagca tcgacaccat
    20401 gttcgctgaa ctggggaaag tttggccgaa atttgacggt ttcgtacact ctattggttt
    20461 tgcacctggc gatcagctgg atggtgacta tgttaacgcc gttacccgtg aaggcttcaa
    20521 aattgcccac gacatcagct cctacagctt cgttgcaatg gcaaaagctt gccgctccat
    20581 gctgaatccg ggttctgccc tgctgaccct ttcctacctt ggcgctgagc gcgctatccc
    20641 gaactacaac gttatgggtc tggcaaaagc gtctctggaa gcgaacgtgc gctatatggc
    20701 gaacgcgatg ggtccggaag gtgtgcgtgt taacgccatc tctgctggtc cgatccgtac
    20761 tctggcggcc tccggtatca aagacttccg caaaatgctg gctcattgcg aagccgttac
    20821 cccgattcgc cgtacagtta ctattgaaga tgtgggtaac tctgcggcat tcctgtgctc
    20881 cgatctctct gccggtatct ccggtgaagt agtccacgtt gacggcggtt tcagcatcgc
    20941 tgcaatgaac gaactcgaac tgaaataatc gttctgttgg taaagatggg cggcgttctg
    21001 ccgcccgtta tctctgttat acctttctga tatttgttat cgccgatccg tctttctccc
    21061 cttcccgcct tgcgtcagga taacgatttc ctttacgacc aaggagcgcc catggaacaa
    21121 cgccacatca ccggcaaaag ccactggtat catgaaacgc aatccagtac tacggagtat
    21181 gacgttctgc ctctggtccc ggaagccgca aaggtcagcg atccctttct gctcgacgtg
    21241 atccttgatg aagagacgct ggcccccttc ctttcatggc tggtccctgc gcgcgttctt
    21301 gcagtggagt tgttccctga ccagcttacc gtgacccgtt cacaaacctt caccgcttat
    21361 gaacgcttgt cgacggccct gacggttgct caggtttgcg gcgtccagcg gttatgtaac
    21421 tactattcgg cgcgacttac gccgctcccc ggacctgatt cctccaggga aagtaatcat
    21481 cggttggcac aaatcacgca atatgcccgc caactggcta gctcgccttc tattatcgac
    21541 aaccgatcgc gccagcatct gaatgacgtc ggtcttactg tctgggactg tgtgatcatt
    21601 aaccaaatca ttggttttat tggctttcag gcgcggacaa ttgcgacatt tcaggcttat
    21661 ctcgggcatc cggtacgctg gttacccggg ctggcgatac aaaactacgc cgacgcgtca
    21721 ctgtttgctg atgaatcaat acgctggcga agcagctatg aggtggaaaa actacctgaa
    21781 gagcacacaa aaagttcaac tgcagaactt tgccaactgg ccgaaacaca ctctctccac
    21841 cctatttcac tttcccttct cgaaaagttg ttaaacagca cacgggtcaa tgcacagccg
    21901 gataatcagc ttgcggcgtt gttatgcgcg cgtataaatg gcagtcctgc ttgtttttcc
    21961 acctgtatgg attcatcaaa tgaatataaa aaaatcagca cccttctgcg caagggcgaa
    22021 aatgaaatta accgatgggc tgaccgtcat tctgttgagc gcgctaccgt tcaggcgata
    22081 caatggctga cccgagcacc cgatcgcttt agcgccgccc agttcagccc tttactcgaa
    22141 cacgaaaaat catcaacgca gattattaat ctgctggtat ggagcgggct gtgtggctgg
    22201 ataaatcgct taaaaatcgc gttgggtgag acatattaac cttgccgcgt cagacagatt
    22261 cgcgtaaaac tgtcagccgc tctaatggcc accaaaatag acaattatgt ttcaggacaa
    22321 cccgctgcta gcgcagctta aacagcaact gcattcccag acgccacgcg ctgaaggggt
    22381 ggtaaaagcc acagaaaaag gctttggctt cctggaagtc gacgcacaaa aaagttattt
    22441 cattccgccg ccgcagatga aaaaagtcat gcatggcgac cgaattatcg cggtgatcca
    22501 cagtgaaaaa gaacgtgaat ccgcagagcc agaagaactg gttgaaccgt tcctgactcg
    22561 tttcgtgggt aaagttcagg gcaaaaatga ccgtctgacc atcgttcctg atcatccact
    22621 tttaaaagac gccattcctt gccgcgcagc ccgtggcctg aaccacgagt ttaaagaagg
    22681 cgactgggcg gttgccgaaa tgcgccgtca tccgctgaaa ggcgatcgtt ctttctatgc
    22741 tgaactgaca caatacatca cttttggtga cgaccacttt gtaccgtggt gggttaccct
    22801 tgcgcgccat aatctggaaa aagaagcacc agacggcgtc gctaccgaaa tgctcgatga
    22861 aggtctggtt cgtgaagatc tgacctcgct ggattttgtc accatcgaca gtgccagcac
    22921 tgaagatatg gatgacgccc ttttcgctaa ggcgctgccg gatgacaaac ttcagctgat
    22981 tgtggcgatt gccgatccaa ccgcgtggat tgctgaaggc agcaagctgg acaaagccgc
    23041 gaaaattcgc gcattcacca actatctgcc tggcttcaac atccctatgc tgcctcgcga
    23101 gctttctgac gatctctgct cactgcgcgc caatgaagtc cgcccggtac tggcatgccg
    23161 catgacgctc tccgctgatg gcaccactga agataatatc gaattctttg ccgccaccat
    23221 cgaatccaaa gcgaagctgg tgtatgacca ggtttctgac tggctggaaa ataccggtga
    23281 ctggaagcct gaaagtgaag caattgccga acaagtccgt ttgctagcgc aaatttgcca
    23341 acgccgcggc gagtggcgtc ataaccacgc actggtgttt aaagatcgcc cggattaccg
    23401 ctttattctc ggtgaaaaag gtgaagtgct ggatatcgtc gccgagcctc gtcgcattgc
    23461 caaccgtatc gtcgaagaag cgatgattgc cgctaacatt tgtgcggccc gcgtactgcg
    23521 cgataagctc ggttttggca tctataacgt gcatatgggc tttgatccgg cgaatgccga
    23581 cgcgctggca gcgttgctga aaacgcacgg tctgcatgtc gatgccgaag aagtgctcac
    23641 gctggacggt ttctgcaaac tgcgtcgtga actggatgcg caaccaactg gtttcctcga
    23701 cagccgcatt cgtcgcttcc agtcatttgc tgaaattagc tctgaacccg gtcctcactt
    23761 tggcctcggt ctggaagcat acgccacctg gacttcgccg atccgtaaat atggcgacat
    23821 gatcaaccac cgtctgctga aagcggttat caaaggcgaa actgcgacgc gtccgcagga
    23881 tgaaatcact gtccaaatgg ccgaacgtcg ccgtctcaac cggatggcag aacgtgatgt
    23941 tggcgactgg ttatacgcac gcttcctgaa agataaagcc gggaccggca cccgtttcgc
    24001 ggcggaaatt gtcgatatca gccgtggcgg catgcgtgtg cgtttggttg ataacggcgc
    24061 tatcgccttt attccggcac ctttcttaca cgctgtgcgc gatgaactgg tttgcagcca
    24121 ggaaaacggc actgtacaaa ttaaaggtga aacggtttac aaagtaactg acgttattga
    24181 cgtcaccatt gccgaagtcc gcatggaaac ccgcagcatt attgcgcgcc cggtcgcgta
    24241 atctcctttc acggcccatt cctcatggat gggccgttta tttccccgct ctaccttcgt
    24301 tatatccagg cagatttttt aactgacttt cgtttgaaaa ctggcgtttt tccaaaactg
    24361 gattacaaat aaatatataa aaaacaatgc gtttagccgc cgttaatttc atgtaagtca
    24421 tgttgttcct tttatttaac cgcggactcc gctgttaacc ggaggatatg catcatgaaa
    24481 accgttaggg agtccacaac gttgtacaac tttctcggat cgcacaatcc atactggcgg
    24541 ttgacggaaa gcagcgatgc tttgcgcttt tctaccacca aaaccacaga acctgatcgt
    24601 acattgcagt tatctgccga acaggctgct cgcatcaggg aaatgacggt catcacctcc
    24661 agcctgatga tgagtctgac cgtcgatgaa agcgatcttt ctgtgcatct ggtaggacga
    24721 aaaatcaata aacgggaatg ggctggcaac gcgtctgcat ggcatgacac accggcggtt
    24781 gctcgtgatt tatcacacgg gctttccttt gctgagcagg tagtttctga agcacattcc
    24841 gcaatagtga ttctcgacag ccgggggaat atccaacgct tcaatcggtt atgtgaagat
    24901 tacacagggt tgaaagaaca cgacgtcatt gggtaaagcg tgtttaaact gtttatgagc
    24961 cgtcgtgaag ctgcggcatc caggcgcaat aaccgtgtat tttttcgaag cggcaatgca
    25021 tatgaagtcg aactgtggat accaacacgt aaaggccagc ggctgtttct gtttcgcaat
    25081 aaatttgtcc acagcggcag tggcaaaaac gagatttttt taatctgttc cggtaccgac
    25141 attaccgaag agcgccgcac tcaggagcga ctgcgtattc tggcaaatac cgacagtatc
    25201 accggactgc cgaatcgtaa cgcaatgcag gagttaatcg atcacgctat taatcaggca
    25261 gataacaata aagttggggt tgtgtatctt gatttggata attttaaaaa ggtcaacgac
    25321 gcctatgggc atttgtttgg tgaccagtta ttacgcgacg tgtcattggc tattttaagc
    25381 tgtctcgaac atgaccaggt gttggcgcgt ccaggtggcg atgagtttct ggtactggca
    25441 tccaatacct cacaaagcgc gctggaagca atggcatcac gaattttgac ccgcttacgc
    25501 ctcccctttc gtattggttt aattgaagtt tataccagct gttcagtagg tatttcgctc
    25561 tctcccgaac atggttcgga cagcgcgact attattcgtc acgccgacac agcaatgtac
    25621 acagcgaagg aaggcggacg aggacaattt tgcgttttta ctccagaaat gaatcagcgg
    25681 gtatttgaat atctctggct ggataccaac ttgcgtaaag cactggaaaa cgatcagttg
    25741 gttatcgaaa atcacctagc gtggcgaagt gcgcagtctg gaagcactag tacgttggca
    25801 gtcacctgaa cgtgggttga ttccaccgtt ggacttcatt tcctacgccg aagagtcagg
    25861 gctaattgtg cctttaggcc gttgggtgat tctcgatgtc gtacgccagg tggcaaagtg
    25921 gcgggataaa ggcataaacc tgcgagtggc ggtaaatatt tctgcacgtc agctcgccga
    25981 tcaaaccatt ttcaccgccc tgaaacaggt tctccaggaa ctcaattttg aatactgccc
    26041 tatagatgtt gaactgacag agagttgtct gattgagaat gatgaactgg cactgtctgt
    26101 tattcaacaa tttagccaac taggtgcgca agtgcatctg gatgattttg gtaccggcta
    26161 ctcttcactt tcgcaactgg cgcgctttcc gatcgatgcc atcaaacttg accaggtttt
    26221 tgttcgagat attcacaaac aacctgtctc gcagtcactg gtccgggcga tcgtcgctgt
    26281 ggcccaggca ttgaatcttc aggtgatcgc cgaaggtgta gagagtgcaa aggaagatgc
    26341 ttttttaacc aagaacggga tcaatgagcg gcaaggattt ttgtttgcca aaccgatgcc
    26401 cgccgtcgcc ttcgaacgct ggtataaacg ctatctgaag cgcgcataag tagatgaata
    26461 tatatactta gcgctatatc ccgtcattat atatcgtatt cctggatgat aattagccgt
    26521 tgctgttaaa cacttctctt ttagcggcaa tttttgcatt attcattttc ccccttttgc
    26581 tataaggatg tccttgatgt ctgaatttga tgcccagcgc gttgccgaac gtattgatat
    26641 tgtgttagat atccttgtgg caggcgatta ccactctgcg atccataatc tcgagatact
    26701 taaagcagaa ctcttgcgcc aggttgctga atctacgcca gacatcccga agacaccgtg
    26761 ggagatttaa caatcgtgcc ttcaggtttt cctatttgag gatgcccggc gcgtcagtat
    26821 ttttttattt agtattataa cgttataaga attacagcga tgaattcccg acaacaaact
    26881 attctacaga tggtcattga ccagggtcag gttagcgtaa ccgatctggc aaaagccact
    26941 ggagtttctg aagttaccat tcgccaggat ctcaacaccc tcgaaaaact gagttacctc
    27001 cgccgtgcac atggctttgc agtttcgctt gatagtgatg acgtcgaaac ccgtatgatg
    27061 agcaactata cgctgaagcg tgaactcgcc gagtttgccg cgtcactggt tcaaccgggc
    27121 gaaaccatct ttatcgaaaa tggcagcagc aatgccctgc tcgcccggac tctgggggag
    27181 cagaagaaaa atgtcactat catcacggtc agcagctaca tcgcgcattt gctgaaagac
    27241 gcgccttgtg aagtcatttt gctcggtggc gtgtaccaga aaaaaagcga aagtatggtt
    27301 ggccctttga cacgccagtg catccagcag gtacatttca gcaaagcatt tattggtatt
    27361 gatggctggc aacctgaaac tggattcacc ggtcgcgata tgatgcgcac cgatgtggtc
    27421 aatgccgtgc tggaaaaaga gtgcgaagcg atagtcctga ctgacagctc gaaatttggt
    27481 gctgtacatt catactccat cggtcccgtt gagcgattca atcgcgtgat taccgattcg
    27541 aaaatacgcg ccagcgatct gatgcatctt gagcacagta aactcaccgt tcacgtcgtt
    27601 gacatttaac catttccctt ctctacggat gatttgcagt ttggcaaatc atccgctcta
    27661 agatgattcc tggttgataa ttaagactat ttacctgtta ttaacactct caagatataa
    27721 aattattatc agcgatataa caggaagtaa ttatcacctg cgtgatataa ccctgcgcgc
    27781 gagcagattt cacggaataa tttcaccagg cttattctta gctattatag ttatagagag
    27841 cttacttccg tgaatcataa attcaggaga gagtattatg tttgtaacga gcaaaaaaat
    27901 gaccgcggct gttctggcaa ttactttggc aatgtctctg agtgcctgtt ctaactggtc
    27961 taaacgggac cgcaacaccg caatcggcgc gggtgcaggg gcattaggcg gtgcagtact
    28021 gaccgatggc agtacgttgg gtacattagg tggtgcagct gtcggtggtg ttattggtca
    28081 ccaggtcggt aaataatcgt aaataaatgt cgattatcga ctcgcaatta taatacgctt
    28141 gattctgttc agtacgaaat aactgaataa tacacacagt aaaatattcg aaagccacgg
    28201 atatatccgt ggctttttat gttaaccgcc tgcgagtttt accttcatcc ctttcgcttc
    28261 aagtagtgat tttaataaat cacgcttatc gccctggatt tcaataactc catctttaac
    28321 tgctccgccg cagccgcatt ttttcttcag ttccgctgca agttttgtca gttcggcatc
    28381 atcgagatcg ataccggtaa tcaggcaaac gcccttacct ttacgtccac tggtctgacg
    28441 ctgaatacgc accgctccat cgcctttagg gcgtactggg gccgctttgg gctcatcaat
    28501 acgcccggtt tccgttgagt agaccagacg gctgttggaa tcactcatca tgcactccgc
    28561 tgtaaagagg cgttgatcgc tttcagtgtc tgcgctggat ctaccgattg cgttaccggg
    28621 cgaccaatca ccatataatc aacaccagcc gccaacgcct gttctggcgt cataatgcgg
    28681 cgctggtcac cagcttcact cccctgcgga cgaatgcccg gtgtaaccag tttgaactcc
    28741 tgaccgaata cctgtttaaa gcgcacagct tcctgagcag aacacaccac accatcaagg
    28801 tcacattttt gcgtcagtgc tgccagacgt tctgcataat ctgcaggtga cagtgtcacg
    28861 ccaagatcgg ccaggtcgct ggcttccatg ctggtcaaca ctgtcacagc aatcaaaagc
    28921 ggtgcatctt tgccaaacgg aaccagtgcc tcacgcgctg cggtcatcat acgcgcccca
    28981 ccggaggcat gaacattcac catccacacg cctaagtcag ctgcagcagc gacagcgtgc
    29041 gctgcagtgt tggggatatc gtggaatttc aggtcaagaa agatatcaaa accacgctgt
    29101 tgaagttcgc gcacaaactg tggcccaaac aatgtaaaca tctctttacc gaccttcaga
    29161 cgacaatcgc gtgggtcgat cttgtcgaca aaggacagcg cgtcatcaca attatgataa
    29221 tcaagggcaa caaccacagg agaattcgta acggcgcggg aagaagatga agcagttaac
    29281 gtcatgacca gaccttcttg atgatgggca ccgggagtgg cgcgaaacag gtaaacggcg
    29341 agcattctac ccgtgaaaat aaaaaattaa cagttgcgat ttttccccta gcaggctttt
    29401 tctgctccgt cgttgccgga gcacaatggg acatcaataa ctagcatgtt gtaactaaag
    29461 tcggattttt ttaaaaatta caggccatca agaccgcgaa tcggtttaat agttgaccag
    29521 gcacgacaag acggacaatg ccagtagagg gtgtatgcag taaaaccaca tttctggcag
    29581 ctataacgag gcttactacg caccttctcg ccaaccatgt cacgcagcac catcagactc
    29641 tctttggcgc gcccttcttc cgcttcattt aagtggtaat ccattagctt atggaataca
    29701 cgcatggtcg gatgacgctg aagctggcgt gtaatataga cctgtgcagc ctcactaccg
    29761 tcgcgcgctt cgatgatatc agcaagcatc aattcagcat cggcaccggt gttctcttcc
    29821 accgcgcgtt gcaggaattc tgcccattcg gcagttttac ccaactgctg atagcaggtt
    29881 tgcaacattt ccagcgtttc gctgaccagt tctctgtcct gggatatgac gcgttgcaaa
    29941 ctttcgacgg ctttggcgta ttctcctttc gccataaaca cgcgtcccat catgatggat
    30001 acgcgggcgc tgtttttatc tgccgccgcc ccttttttta gcaaggtcat ggcacgatcg
    30061 agatcgtcgc tggccatatg ctgcagggct aactcacagt agaaatgggc aatttcgacg
    30121 cgctgtttat ctttacccag cttcaccagg cgttcggcaa catcaattgc tttctgccac
    30181 tcactggtag cctggtagat ttgtagcaac tgttgcagcg cgccaatgcg gaagtcagtt
    30241 tcatcggtca gctgattgaa catgtcttcc gcgcggtcgt ataacccggc ggccatgtaa
    30301 tcacgcccca gttgttgaat cgccaacaga cgctgttcat aggtcagcga ggcgctttcc
    30361 attagggtct gatggatgcg aatagcgcga tcaacttcgc cacgcgaacg gaacaggttt
    30421 ccgagcgtaa ggtgggcttc aacggtgcct gtatcctctt taagcatatc gagaaacagg
    30481 tctaccgctt tatcctgttg attactaagc aggaagttaa cccccgctac gtaatcacgc
    30541 gacaagcggt tggcttcatc ttgcttgttt tgttgcgcac ttctgcggcc catataccag
    30601 ccataggcag cggctacagg caaaagcaga aacaacaact ccagcataga aagttattcc
    30661 ttcgccgctg acgagtgcgg cactacagcc acgtcagtcg cgggtgaaag ctggttttcc
    30721 agtcgcttta ttttacgttc agcgcgcgcc agggaaacac gaactcgcag ccagaacagg
    30781 ccacaaatca accaaccgat agcaaacccc gcagcaaaca ataccgccag caatgtggaa
    30841 atacggtact ccccttgcgc taacagataa ttaaacgtca cctgttgatc gttctgcgca
    30901 cccaacgtga ccgaaatcac gaagatcgct aacaccagta agaaaatgag taaatatttc
    30961 acattacttc ccgttatgtg gttagagcga ataaattatg gtcaacttac cgcaatagcc
    31021 ctttaaagta ccattttcgc gccgtctgcg aaatggcatc cacgcgcatt gttgatgatt
    31081 taaggcctaa attcggaaaa tcaaccagcg ttaactttct tgttctcgtt gtgcaatttc
    31141 gcgattttct tccgcaggtg gtgttaatgg cccacaaatt cgttgcgcaa gccacgttgc
    31201 caccgccacc agcgcccacg aaatcaacgt agctaccacc agatcgcgtg gccaatgcat
    31261 cccgagcagc aggcggcttc ccatgactcc cgttgcccag accaacaaga tagcaatggt
    31321 taacgttcgc cgacgcggcc acagcaaacc aacggccagc agtgcccaac tggcagcaaa
    31381 catcgtgtga ccggaaggaa aggcaaaccc cgtctctttc tgccagtgtg aacgcaaata
    31441 ttgtggaata tttttctctt cagccaactg ttcttttact agatttccgc gttctgctcg
    31501 ctttaaagtg tagaactcat caaccggaat atgatgtgtt ttttccaacc agataacaaa
    31561 aggtcgtggt tcctgtactt tgtctttgat ccaggattta acgccttgtc caacaaggat
    31621 tgccgccgcc agaatggcaa ataatacaaa ggcagcctta atgcgaaaac gcagacacca
    31681 gagaaaccag ccgaacaaaa tcaaatgtgt aatgacgccc cagggctggg tgacagtttc
    31741 agtaacccaa aaagccgctt ttagtagcca actttgttct ccaggttgcc aacgccagcc
    31801 agaaatccat acggctactg gcatgacaag caatagtgca gctcccactg cggtacgtcg
    31861 ggcaatcgaa cgcatggcct ctcctttttg ataagtccca caatcataac tgaaaatgcc
    31921 agttccaaga aaaattgaca gatttgtgcc attccgtgaa cgatcgacgc gtcgtgatta
    31981 ggtgaacccc ttctcgttat ggcaaaataa gccaatacag aaccagcatt atctggagaa
    32041 tttcatgcag cttaaacgtg tggcagaagc caaactgcca accccatggg gcgatttcct
    32101 gatggtggga tttgaagaac tggcaaccgg acacgatcat gtcgcgctag tctatggcga
    32161 tatttccggg cataccccgg tacttgcgcg cgtccattcc gaatgtctga ccggtgacgc
    32221 cctgttcagc ttgcgctgcg attgtggctt ccagctcgaa gcggcattga cgcaaattgc
    32281 cgaggaaggc cgtggtattt tgctgtatca ccgtcaggaa ggtcgtaaca ttggtctgct
    32341 gaataaaatc cgcgcttacg cactgcagga tcaaggttac gataccgtag aggctaacca
    32401 ccagttaggc ttcgccgctg atgagcgcga cttcactctt tgcgctgata tgttcaaact
    32461 ccttggcgtc aatgaagtcc gcttgttaac caataacccg aaaaaagtcg aaattctgac
    32521 cgaagcaggg attaatattg ttgaacgcgt accattgatt gtaggtcgta accccaataa
    32581 cgaacattat ctcgatacca aagccgagaa aatgggccat ttgctgaaca aataaccctc
    32641 ttgcatcgtg taattcattt gcttgccgga agcaaaataa ccggcaagca aatacttgtt
    32701 acttcaacat attacgaatg acataatgca aaatgccgtc gttctggtag taggtcaact
    32761 ccgtcgcggt gtcgatacga caacggcagg gtacgacttc ctggctacca tccgcgcgcg
    32821 taagcgtcac cggaaccgtc gcgccaggtt gtagattttg cagatcgcca atatcaatct
    32881 tctcttcccc ggttagccct aacgttttac gcgtcacgcc ttgcggaaat tccagcggca
    32941 ggatgcccat gccaattaaa ttcgaacggt gaattcgttc aaacgattcg gcaatcacca
    33001 cacgaattcc aagcagacga ggtccttttg ccgcccagtc acggcttgag cctgatccat
    33061 actctttccc ggcaatcacc gccagcggcg tttgctcctg cttatagcgc atcgcagcat
    33121 cataaataga gactacgtcg ctgtcaggta aatgccgcgt catcccccct tcaacgccag
    33181 gcaccatttc attacggatg cgaatattgg cgaaggtgcc gcgcatcatc acttcatggt
    33241 taccacgccg cgaaccgtag gagttaaagt cttttcgctc aacaccccga ccttgtagat
    33301 atcgacccgc tgggctgtcg ggcttaatac tgcccgccgg agagatatgg tcagtggtga
    33361 ctgaatcccc cagcattgcg aggatccgcg caccgtgaat atcttccact ggtgctggtg
    33421 ttgcctgcat ttcatcaaag aaaggcgata agcgaatata ggttgagtcc tcctgccaac
    33481 cgtaggtatc ggatcgtgtg acgttaattt ccttccactc tgctgtgcct tcaaaaactt
    33541 ctgcgtactc tttgcggaac atttctgtgg agacttgttc taccgcacgg gcaatttctt
    33601 gtgccgatgg ccagatatct ttcagataaa ccggctcgcc tttgcgatca tggccgatag
    33661 gctcagaagc caggttgata ttcatatttc ccgccagcgc ataggcaacc accagcggtg
    33721 gcgaggccag ccagttagtt ttaaccagcg gatggatacg gccttcaaag ttacggttgc
    33781 cggacagcac cgcaccgacg gttaaatcgc cttttttgat tgccgtttcg ataggatcgg
    33841 gcagcggccc agagttacca atacaggtgg tacaaccgta tcccacaagg ttaaacccca
    33901 gttcgtcgag atacggtgtc agtttcgctt ttgccagata atcagaaacg actttcgaac
    33961 ccggtgccaa cgacgctttg acccacggtt gccgcttgag gccaagagtt acggcttttt
    34021 tcgccagcaa gcctgcggcc atcagcacac ttgggttaga ggtgttggtg cacgaggtta
    34081 tcgcagcaat gaccacagcg ccatcaggta actgatactg atgtccgttc ataacataat
    34141 cgactggcag gcgatcttta tgcgtggcat tcacttccag ttcgttactg gcggcaaatg
    34201 cttttggtac atcgggcagt gcaacgcgat cctgtgggcg ttttggccct gccaggctcg
    34261 cttcaacgtc attcatatcc agttctaaca tactggtaaa aatcggttca tcgcccgggt
    34321 tacgccacat gccctgcgct ttggcatatt tttcgaccaa ctcgacctga tcttcgctgc
    34381 gcccgcttaa acgcatgtaa tcgagggtta ctgcatcgat tgggaagaag ccacaggtgg
    34441 caccatattc tggcgacata ttggcaatgg tggcgcgatc cgccaacggt agtgaatcca
    34501 gaccatcacc ataaaattcg acgaatttcc ccaccacgcc atgtttgcgc agcatttggg
    34561 taacagtgag aaccaggtct gtggcggtaa taccttcacg taattttccg gtaagtttaa
    34621 agcccactac gtccgggata agcatggaaa ccggctggcc taacatggcg gcttctgctt
    34681 cgatcccacc cacgccccac cccagcacgc caaggccgtt gatcatagtg gtgtgcgagt
    34741 cagtaccaac gagtgtatcc ggataagcaa tccattcacc gtcctgcaat tcgctccaca
    34801 ctgctttgcc gagatattcg aggttaacct gatggcaaat gcctgtgcct ggcggcacga
    34861 cgctaaaccg actgaacgct tgctttcccc atttcaggaa cacataacgt tcgtggttgc
    34921 gctccatttc caggcgtacg ttttcttcaa atgcctcatc atcaccaaaa cgatcgacgg
    34981 tcaccgagtg gtcaatgacc aggtcgaccg gtgagagcgg gttaaccttt gcagtatcgc
    35041 caccgaggcg tttaaccgct tcgcgcattg ccgccagatc aacaacggca ggtacgccgg
    35101 taaagtcctg catcagcacc cttgccgggc ggtaggcaat ttcacggtca gcatgggcat
    35161 ttttcagcca tcccgccagt gcgtggatat cctcttcggt aactgagtta ccatcctgcc
    35221 agcgcagcag gttttcgagc aaaactttga gtgacttggg tagacgggtg atatcgccca
    35281 gtgatttagc agcaagcggc aggctgtagt agtggtaagt tttatctttg gcctgcaacg
    35341 tgtccttact ggcttctcgt agggttgacg acatagctcc tccttaatga cagggttgcg
    35401 aagccctgat tctcttcagg gtgaatatta aagataacac aaacatcgcg taacgatttg
    35461 ataacaaccc aaattgataa aagaggagaa accttgtggc aaagcaaatg acaaccccgc
    35521 cgcagcgggg tcaaggatca gtgagtaatg atccagagga gttcggccca gaagagcatc
    35581 gacagagaaa acgcgcccat ccaggaccaa tatttcatat ttgtgttgat cataggctac
    35641 acccttaacg gttattattt tgtgttgaga taactcaagc tgttattgct gcggtgtatt
    35701 aaaagccctt taattcatta aaaatgggta ataagaatga tgataattat tgtttcggct
    35761 gatcatcttc agcaaacagt tctataaagg cttgctgctg cgatgttagt tgccagtcgt
    35821 ctggaataac gacggaacat tcccgtttcg tttctggttc attgtcacta tgctggcata
    35881 atggtgacga ctccagttgc tcctgaccga ccatagttat ccccacactt tccaaatcag
    35941 cagtgcgaca accacccaga aaagtgcaga accaagaaaa accgccagcc aggctttacg
    36001 ttttaattca ggatcacggc ggggttcctg atttccagaa ggcattgcta acctcatata
    36061 atcgccatca cttatcagca agacgttgaa tgattggtgt aattaatcat acgatgagac
    36121 aaatcctaaa gaaactttag tcaaaaatcc agcgaattta cgaatcgtct ccagtgaata
    36181 attcaatctt ttgacctgaa atagatattt gaaaactgga atttgcgagg cgggtaatta
    36241 gacactactt tacaggtaat ttaccctata aatatgaggg ataaaaatat tgtgcacggt
    36301 atagaaataa gtaacaaaag agaatatatt ccggcacctt cgctacataa aaggtgccga
    36361 aaataacgca agaaattatt tttccggcag ttttatatct ttaaacatga cctcaatttc
    36421 ttcattggag cgcaatgcga cagccgcatc aacgacatca cgcgttaaat gcggtgcaaa
    36481 acgctgaatg aaatcataca tataactgcg caaaaaagta ctacggcgaa aaccaatttt
    36541 ggttgtactg tggctgaaga tatcgtgagc atcaacgcgc acaaggtcgg gatcggcgac
    36601 cggatccacc gccatgctgg caatgacccc tacccccagc cctaaccgga cgtaagtttt
    36661 aatgacgtca gcatccgttg ccgtgaaaac gatacgcggc gttaaccctg cgcggttaaa
    36721 ggcagtatcc agttctgaac gtccggtaaa gccgaaggta tatgtcacca acggatattg
    36781 cgccagttct tcaatggtaa tggctttttt gcctgccagc gggtgatccg gagtgactac
    36841 aatagcccga ttccagtggt agcacggtaa catcactaaa tcttcataca gatgcagcgc
    36901 ttctgtggcg atagcgaaat cggcattgcc tttagagacg gcatcagcaa tttgtgtcgg
    36961 cgggccctgg tgcatatgca aagaaacgcg gggataacgc tcaataaagc ctttgataac
    37021 gtttggtaac gcgtagcgtg cctgggtatg cgtggtggcg atatacagcg agcctttatc
    37081 cggccaggtg tgctcaccgg caaccgattt tatggcatcg actttcgaca ggacttcgcg
    37141 agcgatacga attatttctt gtcctgctgg cgttacctgc gtcaggtgct tgccgcttcg
    37201 ggaaaaaatt tgaatgccta gctcgtcttc cagcattctg acttgtttac tgatcccggg
    37261 ttgtgatgtg taaagtcctt ccgctgttga tgagacattc aggttatgat tgaccacctc
    37321 aacaatatag cgaagttgtt gtaatttcat gttaaaccat ccacttagac gccatcagaa
    37381 catcgttttt tcgtcacacc tggcgggtga ctaatacgat ttaataataa aaaaggtgct
    37441 aactataacc actatatcat ttatagcctt agtgtatagg tgtgaagaaa aaataatatg
    37501 caataaaaaa gggccgcttt cgcgaccctt tgtttataaa aacctgacag aattaaaggt
    37561 tatttttttc cttcaaccca tttgccatca acataaaatg ctgaccagcc agtcgccttt
    37621 ccgtcttttt ccgaagagac atattgctgt ttggttttac ggctaaagcg aaccatggtc
    37681 ttattacctt ccggatcctg ctgtggcgca tcggccagat aacgcagttt ttccggcaga
    37741 cggtcgcgga agcgataaag ctcttccacc agtggcgcac gcgtttcacg cgatttcggg
    37801 aaagtgttgg cagccaggaa cacaccggca gcaccgtcac gcagcacgaa ataagcatct
    37861 gatttttcgc acggcagctc aggtaatggc accggatctt ctttcggtgg tgccacttcg
    37921 ccgttacgta aaatcttacg tgtgttttta cactcttcgt tggtgcaggc catgtattta
    37981 ccgaaacgcc ccattttcag gtgcatttca gagccacatt tttcacactc aacgatcggg
    38041 ccgtcataac ctttaatgcg gaattcgccc tcttcgatct cgtaaccgtc gcaggttggg
    38101 ttattaccac agacatgcaa cttacgtttc ggatcgatga gatagctgtc catcgccgtg
    38161 ccgcatttcg ggcaacgacg ttttgcgcgc agcgcgttgg tttcagcatc ttcgccttcc
    38221 agcacgttca gcacttcgtt ttccggcacc aggttaatgg tggttttgca acgctctttc
    38281 ggcggcagcg catagccaga acagccaagg aataccccgg tactcgctgt gcgaatcccc
    38341 attttgcgac cacaagtcgg gcagtcaatg ctggtcagaa ccatctggtt cgggcgcata
    38401 ccaccctctt ccggatcttt ttcagcttta tctaactgct gggtgaaatc cgagaagaag
    38461 tgatccagta cagctttcca ctctgcttcg tgatttgcca cctggtcgag gctgttttcc
    38521 atctgcgcgg taaagtcgta gttcattaac tcgcggaaat tttcttcaag gcgatcggtg
    38581 acgatttcgc ccattttttc cgcatagaaa cgacgatttt ctactcgcac atagccacga
    38641 tcctgaatgg tcgaaatgat cgacgcatag gtagacggac gaccgatacc acgtttttcc
    38701 agttctttaa ccagcgatgc ttcactgaaa cgggctggcg gcttggtaaa gtgctgggct
    38761 ggtgttagtt caacgagcgt cagagcatcg cctttatcga ctgccggtaa gatacgatct
    38821 tcatcgcctt tacgcagtgc aggcatcact tttgtccagc catcaaagca caaaatacga
    38881 ccgcgtgctt tcaggcggaa atcgcccgca ccaacggtca gcgtcgtgga gtcatatttc
    38941 gctggggtca tctggcaggc aacgaactga cgccagatta actggtacag tttctgcgca
    39001 tctgcttcca tatccttcag cgattccgcc atcacattga cgtcagaagg acgaatcgct
    39061 tcgtgcgctt cctgtgagtt ttctttgctg gcgtactgat tcggactttc cggcagatat
    39121 ttcttaccaa aattatcgct gatataaccg cgaaccatat ttaccgcgtc ctgactcagg
    39181 ttagtggagt cggtacgcat gtaagtgata tagcctgctt catacaaacg ctgcgccatc
    39241 atcatggttt ttttcacgcc aaaaccaaga cgggtgctgg cagcttgttg cagcgtagag
    39301 gtaataaaag gagcgccagg tttactggtt gtcggtttgt cttcacgttc cagcacgctg
    39361 tagcgcgctt tttccagcag acttaccgca gcctgagttt gttctttgtt gaccggacgg
    39421 aacggtttgt cattctgatg agtcacctgc aacgccaacg cttcaccaga tggcgtagtc
    39481 gtgctggcat cgacttccca gaactcttcc ggtacgaacg ctttaatttc acgctcacgc
    39541 tctaccacca ggcggaccgc caccgactgc acacgaccgg cagacagacc acgagcgatc
    39601 tttttccaca gcagcggcga aaccatatac cccaccacgc ggtccataaa gcgacgcgcc
    39661 tgctgggcat taacacgatc aatattcagc tcacccggtt tgttaaatgc ctggcggatc
    39721 gcgtttttag taatttcgtt aaacaccact cggctatagc gcgcatcatc accaccaatc
    39781 acttcccgca ggtgccatgc aatggcttcc ccttcgcggt caaggtcggt tgcgagatag
    39841 atgtggtcgg ctttttcagc cagttgtttc agttcagaga cgaccttctc tttaccaggc
    39901 aacacttcat agtgcgcctc ccaattgtgc cacgggtcaa cccccatacg gttgacgaga
    39961 gcgccacgtt catccttttt aggcttttta gccgtcttgg tggaggtaga gtcggcactc
    40021 tttttggcag ctgagccact ggtcggcaaa tcgcggatgt gaccgacgct ggatttcacc
    40081 acgtagtcac aacccagata cttgttgatc gttttggctt ttgccgggga ctcaacgatg
    40141 acaagagctt tacccatatt caccttacct aatttgattc ttccaggaat gcgtcgcacg
    40201 ttgataaacc ttccactggc aacgagttac cgatatggac tcgctctctg cggatatcaa
    40261 ccccttgtcg tgactctgtt cacgaaaatt caggtgactg agttaacagg tcttggccta
    40321 caggcgctat agcacgacgg aattttggtc gaatgtcaag caaatctatt gccagattga
    40381 cgaaagcgtc acactgtacc tgataaaatc cgatacgcaa ctttattagc atgcaaaatc
    40441 caaagttgcc agtaaggcgc tcttgccacc agcgtggtat atttgtctgc aagccatgcc
    40501 tggcgtagac taatgtcctt gtttaaggag taaataatga acaaagagac acaacccatc
    40561 gaccgcgaaa cgctgttgaa agaggccaac aaaatcattc gtgagcatga ggatacgctc
    40621 gcagggattg aagccaccgg cgtcacgcag cgtaatggcg tgctggtatt taccggcgac
    40681 tactttttag atgaacaagg attgccaaca gccaaaagca ccgcggtgtt taatatgttc
    40741 aaacacctgg cccatgtgct ttcagaaaaa tatcatctgg ttgattaaag caaaacgcga
    40801 ggtcttagcc tcgcgtttgt cttttacatc aatggctttt gaccccgctg ccaccagcgt
    40861 agcaacaatc gatcggcgct ctctgccgcg ctgccggtga atcggtcaat gagtcgttta
    40921 cgctgcatat agcgtacatt aaccacttca cggccttcca tcaggctaag aataacttca
    40981 tcactggtgt tgatttcatc aaccaggcct ttctctaccg cctgttgtcc gtaccagtgt
    41041 tcacccgttg ccacctgttc aatatccaga gacggacgca tacgcttcac aaaatcttta
    41101 aataactgat gcgtttcgtt cagctcttcg cggaatttct cccgcccttc ttcggtattt
    41161 tcacccagca aggtcagcgt gcgcttatac tgcccggcgg tgtgcagttc gatatcaata
    41221 tctttgcttt tcaggaagcg gttaaagttg ggcatttgcg ccaccacccc aatggaaccc
    41281 acaatagcaa acggtgcgga aacaattttg tccgccacac aggccatcat gtaaccgccg
    41341 ctggcagcga ctttgtctac cgtaacagtt aaaggaatgt ttttatcacg cagacgctgc
    41401 agctgcgaag ccgccaaccc gtaaccatgc accatgccac cagggctttc cagacggagc
    41461 acaacctgat cctgcggttt gaatgctgcg agtaccgccg ttatctcttc acgtagcgag
    41521 ttcacttcat gggcgtccat gctgccttta aaatccagca cccagacgcg aggtttactg
    41581 tcagttgcca cctcgcccag tttggctttc gcttttgctg ctttcgcttc ttgcttgtgc
    41641 tttttcttct gcgctttgtg ccactgtttt tgctgatgtg agtccatcag cgccgcggcc
    41701 agttcttctt tcatctcctt atactgttcg ctgagattgt tgacccgtaa ctcgccacgc
    41761 tggcgtttat tacgttgagc aacattgaca ataatggcgg caatcgccgc aatcgctagc
    41821 acaacggtaa cgattttagc caaaaacaaa ccatattcag acaacaattc cacgagtccc
    41881 caccttggtt aaacaacgct ttggctcaca gtttacaaca ggcatgttaa gccgtctcgc
    41941 agagatgtta ttgcagcgaa agtatgacaa agtatccatt ttgcggtgtt atcgccccag
    42001 gctgattgaa atgcccttct gtttcaggca taaagcccca aagtcataaa gtacactggc
    42061 agcgcgacag acaacgtcgt tgaggagtag ccatgcatta ccagccaaaa caagatttac
    42121 tcaatgatcg cattatcctg gtgacgggag ccagcgatgg gattggtcgt gaagccgcga
    42181 tgacgtatgc acgctatggt gcgacagtga ttctgttggg ccgtaatgaa gaaaaattac
    42241 gtcaggtagc cagccacata aacgaagaaa ctgggcgtca gccacagtgg tttattctcg
    42301 atttgctgac ctgcacgtcc gaaaattgcc aacaactggc acagcgcatt gtcgttaatt
    42361 atccgcgtct ggatggtgtt ttgcataatg ccggattgct cggcgatgtt tgcccaatga
    42421 gcgaacaaaa tccgcaggtc tggcaggacg tcatgcaggt caacgttaat gccaccttta
    42481 tgctcaccca ggcactgctt cctttattac tcaaatcgga cgccggttca ctggtcttta
    42541 cttcatcaag cgttggacgt cagggacgag ccaactgggg tgcatatgca gcgtcgaaat
    42601 ttgccaccga agggatgatg caggtactgg ccgatgaata tcagcagcgc ctgcgcgtca
    42661 actgcattaa cccaggcggt acgcgcaccg caatgcgcgc aagcgccttc ccgaccgaag
    42721 atccacagaa acttaaaaca cccgctgata tcatgccgct ctacctctgg ctgatgggcg
    42781 atgacagccg ccgtaaaacc ggcatgacct ttgacgccca accgggccgt aaaccaggaa
    42841 tttcccaatg agtgatgaac gctaccaaca gcgtcagcag cgagtgaaag aaaaagtaga
    42901 tgctcgtgtg gcccaggccc aggatgaacg cggtattatc atcgtcttta ccggcaatgg
    42961 aaaaggcaaa accaccgcgg catttggtac ggcaacacgc gcagttggtc acggaaaaaa
    43021 agtgggcgtc gtgcagttta ttaaaggcac ctggcctaat ggcgaacgca atctgctgga
    43081 gccacatggc gttgagtttc aggtgatggc aacgggcttt acctgggata cacaaaaccg
    43141 cgagtctgat accgccgcct gccgcgaagt ctggcaacat gcaaagcgga tgcttgctga
    43201 ttcctcactg gatatggttt tgcttgatga actgacgtat atggtggcgt atgactattt
    43261 gccactggaa gaagtggtgc aggcgttaaa tgaacgtcca catcaacaga cggtgattat
    43321 cacgggtcga ggctgtcatc gggatattct tgaactggcg gatacggtaa gtgagctgcg
    43381 ccccatcaga catgcgtttg atgccggtgt taaggcgcaa atagggattg attattaatc
    43441 tgtaaaaatg ccggatatga cgtccggcat actttgatta accgttatta cgcccgccag
    43501 aacgacgact gccgctcacc tgactgtgac gtttcaccgc acgacgaatc tgattagcct
    43561 tcatgcgacg acggtctttt tctacagcga ctttagagct ggtttctggc ggaagctcca
    43621 ccagttcgcg cagatagtta gtctgggcga ggtccagctc cgtccagcca ccgcgtggca
    43681 gaccttttgg cagtgggata tcaccgtaac gaacacggat caggcggctc acctgcacac
    43741 caaccgcttc ccacagacga cgaacttcac ggttacggcc ttcggtcaga gtcacgttgt
    43801 accactggtt gatcccttcg ccgccgctga acttgatggt tttaaaagct gccggaccat
    43861 cttccagttg cacgccacga ctcaaatcac gcagtttcgc gtcgtcaacc tgaccaaata
    43921 cacgcacggc gtattcacgt tcaacttcac ggcttggatg cattaaacgg tttgccagtt
    43981 caccatcggt ggtgaacagc agcagaccac aggtgttaac gtccagacga cctacggcaa
    44041 tccagcgtgc gccacgcagc tttggcagac ggtcaaacac cgtcggacgc ccttccggat
    44101 cgttacgggt acacaactca ccttccggtt tgtaataggc cagaacgcga caaatttgtt
    44161 cagcggactc acgtaccgaa atcagatgac catcgatacg aattttcagc ccaggggtaa
    44221 cttcaacgcg atcgccgagt ttagcaattt tgccatcaac actcacacga ccggcttcaa
    44281 taatagattc gatttcacgg cgggagccgt ggccagcgcg ggccagcact ttctgtaatt
    44341 tttcgctcat agagcttcct tttggtgtcg ccttcacagg cgtcgaacag gagaatcaat
    44401 ggcgccgtat agcgccattt taaggccgcg tagtatacag agttacgcac ttataagaaa
    44461 ggcttcacat cacctacgcc ttcacgcacc acgaccggcg tatcatcggt aagatcaata
    44521 accgttgttg gtttctgccc gagatagccg ccgtgaataa tcaaatctac ctgtttttcc
    44581 agacggtctt taatttcttc gggatcggat tcggtaaatt cgctgcctgg cagcattagc
    44641 gaagtggaaa gcatcggttc gcccagtgct tcaagcaacg cctgcgcaat cgggttagaa
    44701 ggcacacgca taccgatggt tttgcgtttc tcctgcaaca gacggcgtgg cacttccttc
    44761 gtccccttca ggatgaaggt atagttgccc ggcgtgttgt ttttcatcaa acgaaacgca
    44821 acgttatcca cgaatgaata ggtcgagagt tcagaaagat cgcgacacat cagggtaaag
    44881 ttgtgaccgt ccggcagctg gcgaatacga caaatacgct ccatggcgtt tttatcttca
    44941 attttacagc cgagcgcata gccggaatca gttggataga caatcacccc gcctttacgc
    45001 acgatctcca ccgcctggtt gatcagacgt tgctgcgggt tatcaggatg aatataaaaa
    45061 aactggctca taattccctc tctgtggtgt tctgcggctg ttcccatagc tgccagacgc
    45121 cttcaacgcc tgcgggcagc cagagtttac gacccagttc gatccacggg catggctgat
    45181 gaaaatcaga tccttgtgat gcccataaat gatgctgacg cgcaagtgtg gccagctggg
    45241 tgcgttcatt gggcgattgc tggcactgcg cgacttccat cgcgtcgccg tggtgttcgg
    45301 caaaatgcgc aaccaatctt ttcagccatt tagcactaag attgtaccgc ccaggatgag
    45361 ctagcaccgc cttaccgcca gaatgatgaa tgacatcaat agcttgttct attgtacacc
    45421 actgtggcgg aacgtatccg gttttcccgc gcgccagata ctttttaaag acatctgcca
    45481 ttgaactggc tttgccgcac tccacaagga aacgcgcaaa atgaccgcgc gtcactgcgc
    45541 cgccctgcgc cagtcgttgt gcgccttcca gcgccccagg tatttgcgct ttttcaagtc
    45601 tttcggcaat ttgctgcgcc cgctgattgc gccgttctgt ctgctgcgcg aggaactcac
    45661 acattagcgg atgagtaata tcaatattca gcccgacaat atgaatttca tgattttccc
    45721 agaccgtgga aatttccacg ccgggaataa gattcagcgc cagtcccgaa cgtgaaattt
    45781 cctctcttgc tggcgcgatt gcagctgtgg tgtcatggtc ggtgatcgcc agggtgccga
    45841 cgcgcatctc gactgcacgg tgcaccaatg cttctggcgt caggcagcca tcggaagctg
    45901 tggtatggct gtgcaggtcg taaatcactg cataattcgt gtcgctcaag gcgcactccc
    45961 gttctggata atgttttttg cgccgacatc ataacgggtc tggcaaatat tctgaaatga
    46021 gctgttgaca attaatcatc gaaccagtta actagtgcgc aagttcacgt aaaaagggta
    46081 tcgacaatga aagcaatttt cgtactgaaa ggttggtggc gcacttcctg aaacgggcag
    46141 tgtattcacc atgcgtaaag caatcagata cccagcccgc ctaatgagcg ggcttttttt
    46201 tgaacaaaat tagagaataa caatgcaaac acaaaaaccg actctcgaac tgctaacctg
    46261 cgaaggcgct tatcgcgaca acccgactgc gctttttcac cagttgtgtg ggaatcgtcc
    46321 ggcaacgctg ctgctggaat ccgcagatat cgacagcaaa gatgatttaa aaagcctgct
    46381 gctgatagac agtgcgctgc gcattacagc attaggtgac accgtcacaa ttcaggcact
    46441 ttccggcaat ggcgaagccc tgctggcact actggataac gccctgcctg cgggtgtgga
    46501 aagtgaacaa tcaccaaact gccgtgtgct gcgcttcccc cctgtcagtc cactgctgga
    46561 tgaagacgcc cgcttatgct ccctttcggt ttttgacgct ttccgtttat tgcagaatct
    46621 gttgaatgta ccgaaggaag aacgagaagc aatgttcttc ggcggcctgt tctcttatga
    46681 ccttgtggcg ggatttgaag atttaccgca actgtcagcg gaaaataact gccctgattt
    46741 ctgtttttat ctcgctgaaa cgctgatggt gattgaccat cagaaaaaaa gcacccgtat
    46801 tcaggccagc ctgtttgctc cgaatgaaga agaaaaacaa cgtctcactg ctcgcctgaa
    46861 cgaactacgt cagcaactga ccgaagccgc gccgccgctg ccagtggttt ccgtgccgca
    46921 tatgcgttgt gaatgtaatc agagcgatga agagttcggt ggcgtagtgc gtttgttgca
    46981 aaaagcgatt cgcgctggag aaattttcca ggtggtgcca tctcgccgtt tctctctgcc
    47041 ctgcccgtca ccgctggcgg cctattacgt gctgaaaaag agtaatccca gcccgtacat
    47101 gttttttatg caggataatg atttcaccct atttggcgcg tcgccggaaa gctcgctcaa
    47161 gtatgatgcc accagccgcc agattgagat ctacccgatt gccggaacac gcccacgcgg
    47221 tcgtcgcgcc gatggttcac tggacagaga tctcgacagc cgtattgaac tggaaatgcg
    47281 tactgaccat aaagagctgt ctgaacatct gatgctggtt gatctcgccc gtaatgatct
    47341 ggcacgcatt tgcacccctg gcagccgcta catcgccgat ctcaccaaag ttgaccgtta
    47401 ttcctatgtg atgcacctcg tctctcgcgt agtcggcgaa ctgcgtcacg atcttgacgc
    47461 cctgcacgct tatcgcgcct gtatgaatat ggggacgtta agcggtgcgc cgaaagtacg
    47521 cgctatgcag ttaattgccg aggcggaagg tcgtcgccgc ggcagctacg gcggcgcggt
    47581 aggttatttc accgcgcatg gcgatctcga cacctgcatt gtgatccgct cggcgctggt
    47641 ggaaaacggt atcgccaccg tgcaagcggg tgctggtgta gtccttgatt ctgttccgca
    47701 gtcggaagcc gacgaaaccc gtaacaaagc ccgcgctgta ctgcgcgcta ttgccaccgc
    47761 gcatcatgca caggagactt tctgatggct gacattctgc tgctcgataa tatcgactct
    47821 tttacgtaca acctggcaga tcagttgcgc agcaatggtc ataacgtggt gatttaccgc
    47881 aaccatattc cggcgcagac cttaattgaa cgcctggcga cgatgagcaa tccggtactg
    47941 atgctttctc ctggccccgg tgtgccgagc gaagccggtt gtatgccgga actcctcacc
    48001 cgcttgcgtg gcaagctgcc cattattggc atttgcctcg gacatcaggc aattgtcgaa
    48061 gcttacgggg gctatgtcgg tcaggcgggc gaaattcttc acggtaaagc gtcgagcatt
    48121 gaacatgacg gtcaggcgat gtttgccgga ttaacaaacc cgctgccggt ggcgcgttat
    48181 cactcgctgg ttggcagtaa cattccggcc ggtttaacca tcaacgccca ttttaatggc
    48241 atggtgatgg cagtacgtca cgatgcggat cgcgtttgtg gattccagtt ccatccggaa
    48301 tccattctca ccacccaggg cgctcgcctg ctggaacaaa cgctggcctg ggcgcagcag
    48361 aaactagagc cagccaacac gctgcaaccg attctggaaa aactgtatca ggcgcagacg
    48421 cttagccaac aagaaagcca ccagctgttt tcagcggtgg tgcgtggcga gctgaagccg
    48481 gaacaactgg cggcggcgct ggtgagcatg aaaattcgcg gtgagcaccc gaacgagatc
    48541 gccggggcag caaccgcgct actggaaaac gcagcgccgt tcccgcgccc ggattatctg
    48601 tttgctgata tcgtcggtac tggcggtgac ggcagcaaca gtatcaatat ttctaccgcc
    48661 agtgcgtttg tcgccgcggc ctgtgggctg aaagtggcga aacacggtaa ccgcagcgtc
    48721 tccagtaaat ccggctcgtc ggatctgctg gcggcattcg gtattaatct tgatatgaac
    48781 gccaataaat cgcgccaggc gctggacgag ttaggcgtat gtttcctctt tgcgccgaaa
    48841 tatcacaccg gattccgcca cgcgatgccg gttcgccagc aactgaaaac ccgcaccctg
    48901 ttcaatgtgc tggggccatt gattaacccg gcgcatccgc cgctggcgtt aattggtgtt
    48961 tatagtccgg aactggtgct gccgattgcc gaaaccttgc gcgtgctggg gtatcaacgc
    49021 gcggcggtgg tgcacagcgg cgggatggat gaagtttcat tacacgcgcc gacaatcgtt
    49081 gccgagctgc atgacggcaa aattaagagc tatcaattga ccgctgaaga ttttggcctg
    49141 actccctacc accaggagca actggcaggc ggaacacctg aagaaaaccg tgacatttta
    49201 acacgcttgt tacaaggtaa aggcgacgcc gcccatgaag cagccgtcgc tgcgaacgtc
    49261 gccatgttaa tgcgcctgca tggccatgaa gatctgcaag ccaatgcgca aaccgttctt
    49321 gaggtactgc gcagtggttc cgcttacgac agagttaccg cgctggcggc acgagggtaa
    49381 atgatgcaaa ccgttttagc gaaaatcgtc gcagacaagg cgatttgggt agaaacccgc
    49441 aaacagcagc aaccgctggc cagttttcag aatgaggttc agccgagcac acgacatttt
    49501 tatgatgcgc tacagggtgc acgcacggcg tttattctgg agtgtaaaaa agcgtcgccg
    49561 tcaaaaggcg tgatccgtga tgatttcgat ccggcacgca ttgccgccat ttataaacat
    49621 tacgcttcgg caatttcggt gctgactgat gagaaatatt ttcaggggag ctttgatttc
    49681 ctcctcatcg tcagccaaat cgccccgcag ccgattttat gtaaagactt cattatcgac
    49741 ccttaccaga tctatctggc gcgctattac caggccgatg cctgcttatt aatgctttca
    49801 gtactggatg acgaccaata tcgccagctt gcagccgtcg ctcacagtct ggagatgggg
    49861 gtgctgaccg aagtcagtaa tgaagaggaa ctggagcgcg ccattgcatt aggggcaaag
    49921 gtcgttggca tcaacaaccg cgatctgcgt gatttgtcga ttgatctcaa ccgtacccgc
    49981 gagcttgcgc cgaaactggg gcacaacgta acggtaatca gcgaatccgg catcaatact
    50041 tacgctcagg tgcgcgagtt aagccacttc gctaacggtt ttctgattgg ttcggcgttg
    50101 atggcccatg acgatttgca cgccgccgtg cgccgggtgt tgctgggtga gaataaagta
    50161 tgtggcctga cgcgtgggca agatgctaaa gcagcttatg acgcgggcgc gatttacggt
    50221 gggttgattt ttgttgcgac atcaccgcgt tgcgtcaacg ttgaacaggc gcaggaagtg
    50281 atggctgcgg caccgttgca gtatgttggc gtgttccgca atcacgatat tgccgatgtg
    50341 gtggacaaag ctaaggtgtt atcgctggcg gcagtgcaac tgcatggtaa tgaagatcag
    50401 ctgtatatcg atacgctgcg tgaagctctg ccagcacatg ttgccatctg gaaagcatta
    50461 agcgtcggtg aaaccctgcc cgcccgcgag tttcagcacg ttgataaata tgttttagac
    50521 aacggccagg gtggaagcgg gcaacgtttt gactggtcac tattaaatgg tcaatcgctt
    50581 ggcaacgttc tgctggcggg gggcttaggc gcagataact gcgtggaagc ggcacaaacc
    50641 ggctgcgccg gacttgattt taattctgct gtagagtcgc aaccgggcat caaagacgca
    50701 cgtcttttgg cctcggtttt ccagacgctg cgcgcatatt aaggaaagga acaatgacaa
    50761 cattacttaa cccctatttt ggtgagtttg gcggcatgta cgtgccacaa atcctgatgc
    50821 ctgctctgcg ccagctggaa gaagcttttg tcagcgcgca aaaagatcct gaatttcaag
    50881 cccagttcaa cgacctgctg aaaaactatg ccgggcgtcc aaccgcactg accaaatgcc
    50941 agaacattac agccgggacg aacaccacgc tgtatctcaa gcgtgaagat ttgctgcacg
    51001 gcggcgcaca taaaaccaac caggtgctgg ggcaggcgtt gctggcgaag cggatgggta
    51061 aaaccgaaat catcgccgaa accggggccg gtcagcatgg cgtggcgtcg gcccttgcca
    51121 gcgccctgct cggcctgaaa tgccgtattt atatgggtgc caaagacgtt gaacgccagt
    51181 cgcctaacgt ttttcgtatg cgcttaatgg gtgcggaagt gatcccggta catagcggtt
    51241 ccgccacgct gaaagatgcc tgtaatgagg cgctacgcga ctggtccggc agttatgaaa
    51301 ccgcgcacta tatgctgggc accgcggctg gcccgcatcc ttatccgacc attgtccgtg
    51361 agtttcagcg gatgattggc gaagaaacca aagcgcagat tctggaaaga gaaggtcgcc
    51421 tgccggatgc cgttatcgcc tgtgttggcg gcggttcgaa tgccatcggc atgtttgctg
    51481 atttcatcaa tgaaaccaac gtcggcctga ttggtgtgga gcctggtggt cacggtatcg
    51541 aaactggcga gcacggcgca ccgctaaaac atggtcgcgt gggcatttat ttcggtatga
    51601 aagcgccgat gatgcaaacc gaagacgggc agattgaaga atcttactcc atctccgccg
    51661 gactggattt cccgtctgtc ggtccacaac acgcgtatct taacagcact ggacgcgctg
    51721 attacgtgtc tattaccgat gatgaagccc ttgaagcctt caaaacgctg tgcctgcacg
    51781 aagggatcat cccggcgctg gaatcctccc acgccctggc ccatgcgctg aaaatgatgc
    51841 gcgaaaaccc ggataaagag cagctactgg tggttaacct ttccggtcgc ggcgataaag
    51901 acatcttcac cgttcacgat attttgaaag cacgagggga aatctgatgg aacgctacga
    51961 atctctgttt gcccagttga aggagcgcaa agaaggcgca ttcgttcctt tcgtcacgct
    52021 cggtgatccg ggcattgagc agtcattgaa aattatcgat acgctaattg aagccggtgc
    52081 tgacgcgctg gagttaggta tccccttctc cgacccactg gcggatggcc cgaccattca
    52141 aaacgccact ctgcgcgcct ttgcggcagg tgtgactccg gcacaatgtt ttgaaatgct
    52201 ggcactgatt cgccagaaac acccgaccat tcccattggc ctgttgatgt atgccaatct
    52261 ggtgtttaac aaaggcattg atgagtttta tgcccagtgc gagaaagtcg gcgtcgattc
    52321 ggtgctggtt gccgatgtgc cagttgaaga gtccgcgccc ttccgccagg ccgcgttgcg
    52381 ccataatgtc gcacctatct tcatctgccc gccaaatgcc gatgacgacc tgctgcgcca
    52441 gatagcctct tacggtcgtg gttacacata tttgctgtca cgagcgggcg tgaccggcgc
    52501 agaaaaccgc gccgcgttac ccctcaatca tctggttgcg aagctgaaag agtacaacgc
    52561 tgcacctcca ttgcaggggt ttggtatttc cgccccggat caggtaaaag cagcgattga
    52621 tgcaggagct gcgggcgcga tttctggttc ggccattgtt aaaatcatcg agcaacatat
    52681 taatgagcca gagaaaatgc tggcggcact gaaagttttt gtacaaccga tgaaagcggc
    52741 gacgcgcagt taatcccaca gccgccagtt ccgctggcgg cattttaact ttctttaatg
    52801 aagccggaaa aatcctaaat tcatttaata tttatctttt taccgtttcg cttaccccgg
    52861 tcgaacgtca acttacgtca tttttccgcc caacagtaat ataatcaaac aaattaatcc
    52921 cgcaacataa caccagtaaa atcaataatt ttctctaagt cacatattcc tcaggtaatt
    52981 gttaatatat ccagaatgtt cctcaaaata tattttccct ctatcttctc gttgcgctta
    53041 atttgactaa ttctcattag cgactaattt taatgagtgt cgacacacaa cactcatatt
    53101 aatgaaacaa tgcaacgcaa cgggagaaat aacatggccg aacatcgtgg tagttcagga
    53161 aatttcgccg aagaccgtga gaaggcatcc gacgcaggcc gtaaaggcgg tcagcatagc
    53221 ggcggtaatt ttaaaaatga tccgcaacgc gcatctgaag cgggtaaaaa aggcggtcaa
    53281 caaagcggtg gtaataaatc aggcaaatcc tgattcaccg ttaattatta atatcagctg
    53341 ataaagataa ttgcatctgc gggccgtatg gctcgcagtg atttcactgg agaaaaatat
    53401 gaatatgaag accattgaag atgtatttat tcaactgctt tcagatacct acagcgcaga
    53461 aaaacaatta acccgggcac tggcaaaact cgcaagagca acatcaaatg aaaaattaag
    53521 tcaggctttt catgcgcacc tcgaggaaac tcatggacag attgaacgaa ttgatcaagt
    53581 tgtggaatca gaatcaaatc tgaaaattaa gcgcatgaaa tgtgtggcaa tggaaggtct
    53641 tattgaagaa gctaatgagg tcatcgaaag taccgagaaa aacgaagtgc gtgatgccgc
    53701 actgattgcc gcagcacaga aagtcgagca ttatgagatt gccagttacg ggacattagc
    53761 gacgctggct gaacaattag gttaccgtaa agcagcgaag cttctgaaag aaaccctgga
    53821 agaagaaaag gccaccgaca tcaaactgac tgatctggcc cttaataacg taaataagaa
    53881 agccgaaaat aaagcctgaa atatgaattt taacttttag tcattttata aagaggacat
    53941 tttcatgaat cgtattgaac attatcatga ctggttacgt gacgcccacg caatggaaaa
    54001 gcaagccgaa tctatgcttg aatccatggc cagccgtata gataattatc ctgaactacg
    54061 cgctcgtatt gaacaacatc ttagtgaaac caaaaaccag attgttcaac tggaaactat
    54121 tcttgatcgt aatgacattt cacgttcagt cattaaagat tccatgagta aaatggctgc
    54181 gcttgggcag tcaatcggtg gtatattccc ttctgatgaa atagttaaag gctctattgg
    54241 cggatatgtc ttcgagcaat ttgaaatcac ctgttacacc tcactattag cagcagcaaa
    54301 aaatgccggt gatacagtct caattccgat catcgaagcg attttaaatg aggaaaagca
    54361 aatggccgac tggctgattc agcatattcc gcaaacaact gagaaatttt taattcgttc
    54421 tgaaactgat ggcgtagaag cgaagaaata atacccttta taccatgtcc ttattgaccc
    54481 cgtatattac ggggtcgttt ttgtgcggaa ttaaaaacga tatcctgctg agaacataaa
    54541 cacccacgga tcgaggcgta cgctatcgtg ttgctgtgca ccgcctagct tataattggc
    54601 ggtggtatcg atatccatgt accacactga catgttaacc aaccagtcac ggttaatcag
    54661 ataatcaacc cccacctgcc cggcagctcc ccaggaatct ttcagactga gatcggaaag
    54721 ccccgcctct ttgccatgat cgttaaatcc attatcaaag aaggtggtgt agttaatacc
    54781 ggccccaacg taaggacgga atttgctgct ggcatcacca aaataccact gcgccatcag
    54841 tgttggcggc agatggtgaa cggttgcaat atcgccggtc gcccgggtgc cgattttatg
    54901 gcggaacggc gtcgctgcca gtaattccac accaatgttg tcggtcgcca tataagtaaa
    54961 cgtcaagccc agttgagtgt tattggtcac gctgaatcca cccagacttc ctaacgtacc
    55021 accagcacct tctgttggac gtacggttgc agaacctgca cgcataaaaa attcgcctgc
    55081 ttcatgcgca aaggcactgc cagaaagaag agttgttact gccaaagccg ccactgttaa
    55141 ctttttcata tccgctccgt cgttatggtt ataaaaacgt gacgaatata cctacattgg
    55201 agtaacaaat gatcctacat agatcacatt acatatgata attcaccatt tattgatctg
    55261 gattaattta gaagttgcat tgagaatcct ggttaatgtg atttaaatca atttttaaat
    55321 attttctcta cgcaaatatt aactcgatcg ccacttgccg cggtttgccc acgcctgaag
    55381 gccagcaatt acctccgctt tacaaagata tgcttttcca tacaagacct gatgctgcca
    55441 cccgaggctt aaccagggta caattgcccg ctaattaaca cctgcataaa actcaaggag
    55501 agtgcatgtc tatcacggcg cagtccgtat accgtgacac cggaaatttc ttccgtaatc
    55561 aatttatgac cattctgttg gtatcgttgc tatgtgcgtt tatcacagtg gtgttagggc
    55621 atgttttctc acccagtgat gcacagcttg cgcagctcaa tgacggcgtg cccgttagcg
    55681 gcagtagtgg gttgttcgac ctggttcaga atatgtcacc ggaacagcaa caaattttgc
    55741 tgcaggcttc agcggcgtcc actttttcag aattaatcgg taacgccatt ctcgccggag
    55801 gcgtaatatt aattatccag ctggtgtctg cgggtcagag agtcagtgcg ctgcgagcta
    55861 ttggtgccag tgcgccgata ttgcccaagt tatttattct gatttttctg actacccttt
    55921 tagtacagat tggcatcatg ctggtggtcg ttccgggaat tatcatggcc attttactgg
    55981 ccctggcacc ggtgatgctg gtacaggaca aaatgggcat ttttgcctcg atgcgtagca
    56041 gtatgcggct gacttgggcg aatatgcgtc tggtggcacc cgcagtactg agctggttgc
    56101 tggcaaaaac actgttgctg ctttttgcct cttcttttgc cgcattaacc ccggaaattg
    56161 gtgccgtact ggcgaacacc ttgagcaacc tgatttctgc catattgctc atctatctgt
    56221 tccgcctgta tatgttgatt cgccaataac cttaagcatt tgattacgga atcgtaaaat
    56281 gaagcagttt cttgattttt taccgctggt tgtctttttc gcgttttaca agatttatga
    56341 catctatgcg gctactgcag cgctgatcgt cgccacggcg attgtgctta tctatagctg
    56401 ggttcgcttt cgtaaggttg agaagatggc cctgatcact tttgttctgg tggtcgtctt
    56461 cggtggcttg acgctgttct tccacaatga tgagtttatt aaatggaagg tgacagtcat
    56521 ttatgctctg tttgcgggag ccctgttagt cagccaatgg gtgatgaaaa agccgctaat
    56581 tcagcggatg ctgagtaaag aactcacgct gccgcaaccg gtatggtcga agctaaatct
    56641 ggcctgggct gttttcttta tcctttgcgg tctggcaaac atctacatcg cattctggct
    56701 gccgcaaaat atttgggtca actttaaagt ctttggcctg accgccctta ccttaatctt
    56761 cacattgtta agcggtatct atatctaccg ccacatgccg caggaagata aatcctaacc
    56821 agactgccag tccttcgggg ctggcgctgt atctcctctg ttctcctcag gaaaatcata
    56881 gtagcatcgc gcctgtgatt ttccttttaa gtcggtttta ccatgtctac aacacataac
    56941 gtccctcagg gcgatcttgt tttacgtact ttagccatgc ccgccgatac caatgccaat
    57001 ggtgacatct ttggtggttg gttaatgtca caaatggata ttggcggcgc tattctggca
    57061 aaagaaattg cccacggtcg cgtagtgact gtgcgggttg aaggaatgac tttcttacgg
    57121 ccggttgcgg tcggcgatgt ggtgtgctgc tatgcacgct gtgtccagaa agggacgaca
    57181 tcggtcagca ttaatattga agtatgggtg aaaaaagtag cgtctgaacc aatcgggcaa
    57241 cgctataaag cgacagaagc attatttaag tatgtagcgg ttgatcctga aggaaaacct
    57301 cgcgccttac ctgttgagta atagtcaaaa gcctccggtc ggaggctttt gactttctgc
    57361 ttactgaatt tcggtggtgc cgttaatttt aaacaggata ttcaccacaa tcccactgcc
    57421 tggcttaccc ggctcataac gccatctgcg catcgcattt ttcacctcac gctcaaacat
    57481 gttcgcaggc ttggctgaga ggatttgtac gttatccacg cgaccatccg gcgtgacgtc
    57541 aaatttaact ttaacctgcc cttcaatgcg caatgcctgt gctcgtgccg gatactgcgg
    57601 ctgattacgg cttaatgcgc gtggtcctga agccacactg gtaaccggct tgctggttgc
    57661 agccgttgcc gtacttgatg tggggcgcgc gggtgccgta ttttcaaacg gtgatgccgg
    57721 acgcgactct acgggtttga catcgcgttt tggctgctcc tgtacctttt tcaccggctt
    57781 cggttttggc ttcggtttcg gcttcggctt ttcaatgacc accggtgctt cttttggcgg
    57841 ttcggggatc ggctcaggtt ccggttctgg ctctaccacc ggctccggtg gcggctgaac
    57901 ggcttgtggc ggttcgagat cagcaggcgc aaccatcgtg acagaaattg gttgcgcagg
    57961 cgcaggtagt tcaataacct gatgtaccga ggtatagagc agccccgcca caacagcacc
    58021 atgaatgcag accgaaagta acgtcggcca ggggaagcgg cgaggtaaat caagggttat
    58081 tgaagtcata atcatttcag tagaaaaact aggcctcgat tttaaatgca aatagcaatc
    58141 atattcaata aggcaagacg atcaggatca gtgaaaatgt catttttggc gtaattaccc
    58201 ttactttatg catgacctta acaaagacat catctttccg ttgcagatct tcgccctttc
    58261 aaataacgta ctttacaact ttcccgaaca aggagttgtg cccgtgttgt atgttattta
    58321 cgctcaagac aaagctgata gcctcgaaaa acgcctttcc gttcgtccgg cacatttagc
    58381 acgtttacag ttactgcatg atgaaggtcg tttgttgaca gctggtccaa tgccagcggt
    58441 agacagtaac gatccagggg ctgcgggatt tactggctca accgttattg ctgaatttga
    58501 atcgctggaa gcagctcagg cctgggccga tgctgacccg tacgtagcgg caggagtcta
    58561 tgaacacgtt tcagtgaaac catttaagaa agtgttctga g
//
LOCUS       DAJTQY010000015        58049 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.015, whole genome shotgun sequence.
ACCESSION   DAJTQY010000015 DAJTQY010000000
VERSION     DAJTQY010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 58049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58049
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.015"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(24..278)
                     /locus_tag="OO349_000939"
     CDS             complement(24..278)
                     /locus_tag="OO349_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HCR5866109.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            433..1005
                     /gene="yeiP"
                     /locus_tag="OO349_000940"
     CDS             433..1005
                     /gene="yeiP"
                     /locus_tag="OO349_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HCR5866110.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLAKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            1228..2694
                     /locus_tag="OO349_000941"
     CDS             1228..2694
                     /locus_tag="OO349_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCR5866111.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERSLTPFTVLSCDNIPDNGHVVKNAVLRMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFIAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAYISDCMQDRVFRYAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPSLKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            2812..3798
                     /gene="yeiR"
                     /locus_tag="OO349_000942"
     CDS             2812..3798
                     /gene="yeiR"
                     /locus_tag="OO349_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HCR5866112.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            3837..4550
                     /gene="lpxT"
                     /locus_tag="OO349_000943"
     CDS             3837..4550
                     /gene="lpxT"
                     /locus_tag="OO349_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HCR5866113.1"
                     /translation="MIKNLPQIVLLNIVGPALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            4962..5528
                     /gene="mepS"
                     /locus_tag="OO349_000944"
     CDS             4962..5528
                     /gene="mepS"
                     /locus_tag="OO349_000944"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HCR5866114.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            5709..7265
                     /locus_tag="OO349_000945"
     CDS             5709..7265
                     /locus_tag="OO349_000945"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HCR5866115.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILGSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVTEGVETPEQARWLSERGVNFMQGYWISHPLPLDDFVR
                     WLKKPYTPQW"
     gene            7347..9161
                     /locus_tag="OO349_000946"
     CDS             7347..9161
                     /locus_tag="OO349_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR5866116.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYTFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFLTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYHVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRAVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRNRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            9162..10256
                     /locus_tag="OO349_000947"
     CDS             9162..10256
                     /locus_tag="OO349_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HCR5866117.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSTFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HMFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            10256..11281
                     /locus_tag="OO349_000948"
     CDS             10256..11281
                     /locus_tag="OO349_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5866118.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            11283..12872
                     /gene="yejF"
                     /locus_tag="OO349_000949"
     CDS             11283..12872
                     /gene="yejF"
                     /locus_tag="OO349_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HCR5866119.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LITSQGSIVFDGQPLQDLNRRQLLPTRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(12876..13220)
                     /locus_tag="OO349_000950"
     CDS             complement(12876..13220)
                     /locus_tag="OO349_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5866120.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(13553..14743)
                     /gene="bcr"
                     /locus_tag="OO349_000951"
     CDS             complement(13553..14743)
                     /gene="bcr"
                     /locus_tag="OO349_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HCR5866121.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVSA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIALLMAPIVGGWVLVW
                     LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFYIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISAPLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(14771..15466)
                     /gene="rsuA"
                     /locus_tag="OO349_000952"
     CDS             complement(14771..15466)
                     /gene="rsuA"
                     /locus_tag="OO349_000952"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HCR5866122.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            15616..17376
                     /locus_tag="OO349_000953"
     CDS             15616..17376
                     /locus_tag="OO349_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCR5866123.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDNPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            17501..17785
                     /gene="rplY"
                     /locus_tag="OO349_000954"
     CDS             17501..17785
                     /gene="rplY"
                     /locus_tag="OO349_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HCR5866124.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(17924..18931)
                     /gene="yejK"
                     /locus_tag="OO349_000955"
     CDS             complement(17924..18931)
                     /gene="yejK"
                     /locus_tag="OO349_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HCR5866125.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            19113..19340
                     /locus_tag="OO349_000956"
     CDS             19113..19340
                     /locus_tag="OO349_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HCR5866126.1"
                     /translation="MPQISRYSNEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFASALQSSINEDKAH"
     gene            19360..21120
                     /gene="pbgA"
                     /locus_tag="OO349_000957"
     CDS             19360..21120
                     /gene="pbgA"
                     /locus_tag="OO349_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HCR5866127.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDDQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQIKRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            21195..21271
                     /locus_tag="OO349_000958"
     tRNA            21195..21271
                     /locus_tag="OO349_000958"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21229..21231,aa:Pro,seq:ggg)
     gene            complement(21374..23965)
                     /gene="yejO"
                     /locus_tag="OO349_000959"
     CDS             complement(21374..23965)
                     /gene="yejO"
                     /locus_tag="OO349_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HCR5866128.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVILE
                     KDIQLVYGTANNTKINPGGEQHIKEFGVSSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAANQTTLNNGVLQVYGPANDTTIKGGRLIVEKDGGTVFAAIEKGGLLEVKEGG
                     FALAVDQKAGGAIKTTTRAMEVFGTNRLGQFDIKNGIANNMLLENGGSLRVEENDFAY
                     NTTVDSGGLLEVMDGGTATGVDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYE
                     LDDGSGLIVMEDTQAIDTILDEHATMQSLGKDTGTKVQANAVYDLGRSDQNGSITYSS
                     KAISENMVINNGRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGAS
                     FRTHGAVDTSKADVSLENSAWTIIADITTTNQNTRLNLANLAMSGANVIMMAEPVTRS
                     SVTASAENFITLTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASP
                     AAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTT
                     DVLNMAAAQPLVFDAELDTVRERLGSVKGVSYDTVMWSSAINTRNNVTTDAGAGFEQT
                     LTGLTLGIDSRFSREESSTIRGLFFGYSHSDIGFDRGGKGNIDSYTLGAYAGWEHQNG
                     AYVDGVVKVDRFANTIHGKMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAF
                     TGFTTDGQDYTLSNGMRADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEY
                     ADSNQVKVNDDGKFNNDVAGTSGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQ
                     AGVVWTF"
     gene            24285..24932
                     /gene="narP"
                     /locus_tag="OO349_000960"
     CDS             24285..24932
                     /gene="narP"
                     /locus_tag="OO349_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HCR5866129.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGEGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRTGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(25143..26195)
                     /gene="ccmH"
                     /locus_tag="OO349_000961"
     CDS             complement(25143..26195)
                     /gene="ccmH"
                     /locus_tag="OO349_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HCR5866130.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(26192..26749)
                     /gene="dsbE"
                     /locus_tag="OO349_000962"
     CDS             complement(26192..26749)
                     /gene="dsbE"
                     /locus_tag="OO349_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HCR5866131.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEKIKPLWEKYSKEAAQ"
     gene            complement(26746..28689)
                     /gene="ccmF"
                     /locus_tag="OO349_000963"
     CDS             complement(26746..28689)
                     /gene="ccmF"
                     /locus_tag="OO349_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HCR5866132.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLATAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            complement(28686..29165)
                     /gene="ccmE"
                     /locus_tag="OO349_000964"
     CDS             complement(28686..29165)
                     /gene="ccmE"
                     /locus_tag="OO349_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HCR5866133.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            complement(29162..29371)
                     /gene="ccmD"
                     /locus_tag="OO349_000965"
     CDS             complement(29162..29371)
                     /gene="ccmD"
                     /locus_tag="OO349_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HCR5866134.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            complement(29368..30105)
                     /gene="ccmC"
                     /locus_tag="OO349_000966"
     CDS             complement(29368..30105)
                     /gene="ccmC"
                     /locus_tag="OO349_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HCR5866135.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(30147..30809)
                     /gene="ccmB"
                     /locus_tag="OO349_000967"
     CDS             complement(30147..30809)
                     /gene="ccmB"
                     /locus_tag="OO349_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HCR5866136.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(30806..31423)
                     /gene="ccmA"
                     /locus_tag="OO349_000968"
     CDS             complement(30806..31423)
                     /gene="ccmA"
                     /locus_tag="OO349_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HCR5866137.1"
                     /translation="MLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQTLLWIGHQPGIKTRLTALENLHFYHRDG
                     DAAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTTIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            complement(31442..32044)
                     /gene="napC"
                     /locus_tag="OO349_000969"
     CDS             complement(31442..32044)
                     /gene="napC"
                     /locus_tag="OO349_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HCR5866138.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSHLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            complement(32054..32503)
                     /gene="napB"
                     /locus_tag="OO349_000970"
     CDS             complement(32054..32503)
                     /gene="napB"
                     /locus_tag="OO349_000970"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HCR5866139.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            complement(32500..33363)
                     /gene="napH"
                     /locus_tag="OO349_000971"
     CDS             complement(32500..33363)
                     /gene="napH"
                     /locus_tag="OO349_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HCR5866140.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            complement(33350..34045)
                     /gene="napG"
                     /locus_tag="OO349_000972"
     CDS             complement(33350..34045)
                     /gene="napG"
                     /locus_tag="OO349_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HCR5866141.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            complement(34052..36538)
                     /gene="napA"
                     /locus_tag="OO349_000973"
     CDS             complement(34052..36538)
                     /gene="napA"
                     /locus_tag="OO349_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HCR5866142.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNSRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            complement(36535..36798)
                     /gene="napD"
                     /locus_tag="OO349_000974"
     CDS             complement(36535..36798)
                     /gene="napD"
                     /locus_tag="OO349_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HCR5866143.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            complement(36788..37282)
                     /gene="napF"
                     /locus_tag="OO349_000975"
     CDS             complement(36788..37282)
                     /gene="napF"
                     /locus_tag="OO349_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HCR5866144.1"
                     /translation="MKIDASRRGILTGCWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGTGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQAQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            37391..37555
                     /locus_tag="OO349_000976"
     CDS             37391..37555
                     /locus_tag="OO349_000976"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5866145.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKHIYIAINSPEI
                     FLLVCSLIRI"
     gene            37690..38178
                     /gene="eco"
                     /locus_tag="OO349_000977"
     CDS             37690..38178
                     /gene="eco"
                     /locus_tag="OO349_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708105.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HCR5866146.1"
                     /translation="MKTILPEVLFAAFATTSAWAAESVQPLEKIAPYPQAETGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAE
                     VR"
     gene            complement(38328..39347)
                     /locus_tag="OO349_000978"
     CDS             complement(38328..39347)
                     /locus_tag="OO349_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HCR5866147.1"
                     /translation="MNYGEITRQLIASLQKKSNFSLQLSSEVRALKRNDDNTWTVTVA
                     DLKNGTAQNIRAKFVFIGAGGAALKLLQESGIPEAKDYAGFPVGGQFLVSENPDVVNR
                     HLAKVYGKASVGAPPMSVPHIDTRVLDGKRVVLFGPFATFSTKFLKNGSLWDLMSSTT
                     TSNVMPMMHVGLDNFDLVKYLVSQVMLSEEDRFEALKEYYPQAKKEDWRLWQAGQRVQ
                     IIKRDAEKGGVLRLGTEVVSDQQGTIAALLGASPGASTAAPIMLNLLEKVFGDRVSSP
                     QWQATLKAIVPSYGRKLNGDVAATERELQYTSEVLGLKYDKPQAADSTPKPQPVQKEV
                     ADIAL"
     gene            complement(39581..39940)
                     /locus_tag="OO349_000979"
                     /pseudo
     CDS             complement(39581..39940)
                     /locus_tag="OO349_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012906537.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
     gene            complement(40158..41801)
                     /gene="yojI"
                     /locus_tag="OO349_000980"
     CDS             complement(40158..41801)
                     /gene="yojI"
                     /locus_tag="OO349_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HCR5866148.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELGQGIILTIGSSAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAVDQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            complement(41877..42527)
                     /gene="alkB"
                     /locus_tag="OO349_000981"
     CDS             complement(41877..42527)
                     /gene="alkB"
                     /locus_tag="OO349_000981"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HCR5866149.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVAPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            complement(42527..43591)
                     /gene="ada"
                     /locus_tag="OO349_000982"
     CDS             complement(42527..43591)
                     /gene="ada"
                     /locus_tag="OO349_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HCR5866150.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            complement(43665..44720)
                     /gene="apbE"
                     /locus_tag="OO349_000983"
     CDS             complement(43665..44720)
                     /gene="apbE"
                     /locus_tag="OO349_000983"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HCR5866151.1"
                     /translation="MEISFTRVALLVAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            complement(44832..45953)
                     /gene="ompC"
                     /locus_tag="OO349_000984"
     CDS             complement(44832..45953)
                     /gene="ompC"
                     /locus_tag="OO349_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCR5866152.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRSTDFFGL
                     VDGLNFAVQYQGKNGSPEGEGMTNNGREALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNFGLNRYDERYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGNLGWA
                     NKAQNFEAVAQYQFDFGLRPSLAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYYFN
                     KNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            46691..49363
                     /gene="rcsD"
                     /locus_tag="OO349_000985"
     CDS             46691..49363
                     /gene="rcsD"
                     /locus_tag="OO349_000985"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HCR5866153.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTRQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESVPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVEE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRLLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            49380..50030
                     /gene="rcsB"
                     /locus_tag="OO349_000986"
     CDS             49380..50030
                     /gene="rcsB"
                     /locus_tag="OO349_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HCR5866154.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(50230..53079)
                     /gene="rcsC"
                     /locus_tag="OO349_000987"
     CDS             complement(50230..53079)
                     /gene="rcsC"
                     /locus_tag="OO349_000987"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HCR5866155.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPCVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TVMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRDS"
     gene            complement(53354..54130)
                     /locus_tag="OO349_000988"
     CDS             complement(53354..54130)
                     /locus_tag="OO349_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HCR5866156.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(54135..55784)
                     /locus_tag="OO349_000989"
     CDS             complement(54135..55784)
                     /locus_tag="OO349_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HCR5866157.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPSVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVTRVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QELIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            complement(55785..56344)
                     /locus_tag="OO349_000990"
                     /pseudo
     CDS             complement(55785..56344)
                     /locus_tag="OO349_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112876.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
     gene            complement(56503..>58049)
                     /locus_tag="OO349_000991"
     CDS             complement(56503..>58049)
                     /locus_tag="OO349_000991"
                     /inference="COORDINATES: protein motif:HMM:NF012433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HCR5866158.1"
                     /translation="LTSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRN
                     NVRTSSSLSFISYDQALSSEPVAPGATNRSERRVKMPERPRREEVDTAAWMPSLTTDK
                     QGKAYFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVYRVGDK
                     PAAGLFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLTQNIQQSGLLSAELQQ
                     NGQVQDSISTKLSFVDNSWPVEQQKNVMLGGGENALMLPEQASNIRLQSSETPQEIFR
                     NNLDALVDEPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAG
                     PGARFTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPL
                     LHRALVLAWAQEMNLPCKTLLKGGDEAIARRGTKDEDFSEEDIRDINDSLILDTPESP
                     LADAVANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQT
                     AAILMV"
BASE COUNT        14198 a        15658 c        14266 g        13927 t
ORIGIN      
        1 tgcttactgc ggattaaaga tgtttaaggc gcggtcagca tttccagatc gataagccag
       61 gtcatggcct gctgacgcga gttaccgcac atttcagcgc tggcttgtaa gcctgcacag
      121 actttcggtc gcagtggcga ggtgaaaatt ttacagcgtt gctgttcatc aagctgaatg
      181 cagggcgtat tggcgggctt gccatctggc ataccgggaa tagggctgga aattgaaggg
      241 gcagtgcaac acgccccgca acccggacgg cattccatac gttgttctct taaggttaac
      301 caccggattg atgagcgcgc acagtaccac cttttttgca ccagcaaaag tgcgaatacc
      361 acttgcccga aaggcccgtc gcgagtactt tgtcgcgata tttttgacat tttcgactac
      421 aggaattttt cgatgccaag agcgaacgaa attaaaaaag gtatggtact gaattacaac
      481 ggcaaactgc tgctggctaa ggatattgat attcagtcgc ccactgcccg cggtgccgct
      541 acgctgtaca aaatgcgttt ttctgatgtc cgtaccgggc tgaaagtaga agagcgtttc
      601 aaaggtgatg atatcgttga caccgtgacg ctgacccgcc gttacgttga cttctcctat
      661 gtcgatggca acgaatatgt ctttatggat aaagaagact ataccccgta taccttcacc
      721 aaagatcaga ttgaagaaga gttgctgttt atgccagagg gcggcatgcc ggacatgcag
      781 gtgctgacct gggacggtca actgctggcg cttgagctgc cgcagaccgt tgatctggaa
      841 atcgttgaaa ccgcaccagg catcaaaggg gcatctgcca gcgcccgtaa caaaccggcg
      901 acattgagca ctggtctggt gattcaggta ccagaatact taagcccggg cgaaaaaatt
      961 cgtatccata tcgaagaacg ccgttatatg gggcgtgctg actaacttca gccgcatgca
     1021 gaaaagggat agctcaggct gtcccttttt taatttatta taccaatctt ctattttgcg
     1081 cttcctgaac accttacgcc ctcaattttc actcgttgat tgatcgccct cacacttcat
     1141 cgcattaaca atccagacca atttcaattg ctgtcatata actttacact gtcattgtta
     1201 atttatcgtt actaagacgt gactcctatg aagacaattg cctccgttac gctcccgcat
     1261 catgtacacg ctccacgcta cgatcgccag cagttgcaat cacgtatcgt tcattttggc
     1321 tttggcgcct ttcaccgcgc tcatcaggcg ttactgaccg atcgtgtgct gaatgcccag
     1381 ggcggcgact gggggatctg tgaaatcagc ttgttcagcg gtgatcaact gatgagccag
     1441 ctccgcgcac agaaccattt atataccgtg ctggagaaag gtgcggacgg caatcaggtg
     1501 ataattgtcg gtgccgttca cgaatgcctt aatgcaaaac tggattcctt agcggcaatt
     1561 attgagaaat tttgcgagcc acaggtggca attgtttccc tgacgattac cgaaaaaggc
     1621 tattgtattg acccggccac cggtgcactc gacaccagta atccgcggat tattcacgat
     1681 ctacaaaccc ctgaagaacc tcactccgca ccgggtattc tcgtcgaagc actgaaacgc
     1741 cgccgtgagc gcagccttac accgtttacc gtgctctcct gcgacaatat tcccgacaat
     1801 ggccatgtgg tgaaaaacgc ggtgctgaga atggcagaaa aacgttcgcc agaactcgcc
     1861 gggtggataa aagaacacgt cagttttccg ggaaccatgg tcgaccgcat tgttccggct
     1921 gcaaccgacg aatcactggc agaaatcagc cagcatctgg gggtaaatga tccctgcgcg
     1981 attagctgcg aaccgtttat ccagtgggtg gttgaagata acttcatcgc tgggcgtcct
     2041 gcctgggaag tcgcaggtgt acaaatggtg aatgatgtcc tgccatggga agagatgaaa
     2101 ctgcgtatgc ttaatggcag ccactctttt ctcgcttatc tgggttacct ctcaggattc
     2161 gcctatatca gtgattgcat gcaggatcgc gtatttcgct atgctgccag aacattaatg
     2221 ctggatgagc aagcgccgac actgcaaatt aaagatgtcg atttaacaca atatgcggat
     2281 aagttaattg cacgttttgc taatccgtcg ctgaaacata agacctggca aatcgcgatg
     2341 gatggcagcc agaaattacc gcaacgtatg ctggcaggta ttcgcataca tctggggcgt
     2401 gaaacggact ggtcgttgct ggcattaggc gttgctggct ggatgcgtta cgtcagcggc
     2461 gttgatgatg ccggaaatgc cattgatgtt cgcgatccgc ttagcgataa aattcgcgaa
     2521 cttgttgcgg gcagcagcag tgaacaacgc gtaaccgccc tgctttccct gcgtgaagtt
     2581 ttcggtgatg atctgccaga taacccgcat tttgtgcagg ccatcgaaca agcctggcaa
     2641 caaatcgtac aattcggcgc acatcaggcg ctattaaaca ccctcaaaat ttaacgattt
     2701 ctgcggttaa agcggatgaa gctcaccttc gtccgctctc cccttctctt ttctgccttt
     2761 tttagccagg attaacgctc agttaactta ccagagtatt gcggagccgt tgtgaccagg
     2821 accaacctca tcaccggttt tctcggcagc gggaaaacca cgtcgattct tcatctgtta
     2881 gcccataaag atcccaacga aaaatgggcg gtactggtta atgaatttgg ggaagtcgga
     2941 attgatggtg ctttgctcgc cgatagcggc gcattgctga aagagatccc cggcggctgc
     3001 atgtgctgcg ttaatggttt acccatgcag gtagggttga ataccttact gcgtcaggga
     3061 aaaccagacc gcttgttgat agagccgacc gggctgggcc atccgaaaca gatcctcgat
     3121 ctgttaaccg caccagtcta tgaaccgtgg atagatctgc gcgccacctt gtgcattctc
     3181 gatccacgcc tgctgctgga cgaaaaaagc gccagcaatg aaaacttccg tgaccagctg
     3241 gctgccgcag acatcattgt cgccaataaa tccgaccgtg cgacgcccga aagtgagcaa
     3301 gcgctacagc gttggtggca gcaaaatggt ggcgatcgac aattaattca cagtgagcat
     3361 gggaaagttg acggtcatct tctggatttg ccgcgtcgca atttagccga gttgcccgcc
     3421 agcgccgcgc attctcatca gcatgtcgtg aaaaaagggt tagcagcgtt aagcctgcca
     3481 gagcatcaac gctggcgtcg cagtctgaac agcgggcaag gatatcaggc ctgcggctgg
     3541 atattcgacg ctgatacggt attcgacacc attggcattc tggaatgggc gcgacttgca
     3601 ccggtggaac gcgtcaaagg cgtgctgcgt attcccgaag ggctggtacg aatcaaccgt
     3661 cagggcgatg acctgcacat tgaaacgcaa aacgttgcgc caccggacag ccgtattgag
     3721 ctgatttcca gcagcgaagc tgactggaat gccttgcaga gcgcgctgtt gaagcttcgt
     3781 ttagcgacta ccgcgtaagg ttgcctgcgt ttttcagtaa gataattaga gaaaatatga
     3841 ttaaaaattt gccgcaaata gtgttgttga atattgtcgg ccccgcgctg tttctttcct
     3901 ggtatattcc cgttaatcat ggattctggt tgccgattga tgcggatatt ttttatttct
     3961 ttaatcagaa actggtcgaa agtaaggcct ttttatggct ggttgcattg actaacaatc
     4021 gcgccttcga cggttgttca ctgctggcga tgggtatgtt gatgctgagt ttctggctga
     4081 aagaaaacgc ccctggcaga cgacgtatcg tgattatggg tctggtcatg ctattaactg
     4141 cagtggtatt aaaccagctg ggtcaggcat taattcctgt aaaacgggcc agcccaacat
     4201 tgacttttac cgatattaac cgcgtcagcg aactgctctc tgttcccacg aaagatgcct
     4261 cacgagatag ctttcccggc gatcacggca tgatgctgct tattttttcg gcattcatgt
     4321 ggcgttattt cggcaaagtt gcaggcctta tcgcccttat tatttttgtg gtttttgcat
     4381 ttcccagagt aatgattggc gcacactggt ttactgacat cattgtcggt tcgatgaccg
     4441 tgatattgat cggtttgccc tgggtgttgc tgacgccatt aagtgatcga ttaatcacct
     4501 tttttgacaa atcactacca ggaaaaaaca aacatttcca aaacaaataa ctcacagtaa
     4561 ttaacatcat cagggttatt tttatagtga ggataatcct gatgatgcgc accgtgcttt
     4621 catctatcga acgcaaaaat cattctctaa gtaaatgaat ggattgcatg cgtttcactc
     4681 aattgtactt taattgacca accccgctta ttaactttct gtatcacttt ttcttataaa
     4741 aaatcatgta aaaccgctcg ccaagaccgc accaatcggg taatctcgaa ctcgttttgc
     4801 ctcggcggta gattatcctc acagcatata attttgtgcg ttagtccaca gatttggcct
     4861 taaggaattg tttcaacatg cccaggtaat tagtctcgtg tcgcttggca tttttttata
     4921 acgatatttg tcgttaagga cttcaaggga aaacaaacaa catggtcaaa tctcaaccga
     4981 ttttgagata tatcttgcgc gggattcccg cgattgcagt agcggttctg ctttctgcat
     5041 gtagtgcaaa taacaccgca aagaatatgc atcctgagac acgtgcagtg ggtagtgaaa
     5101 catcatcact gcaagcttct caggatgaat ttgaaaacct ggttcgtaat gtcgacgtaa
     5161 aatcgcgaat tatggatcag tatgctgact ggaaaggcgt acgttatcgt ctgggcggca
     5221 gcactaaaaa aggtatcgat tgttctggtt tcgtacagcg tacattccgt gagcaatttg
     5281 gcttagaact tccgcgttcg acttacgaac agcaggaaat gggtaaatct gtttcccgca
     5341 gtaatttgcg tacgggtgat ttagttctgt tccgtgccgg ttcaacggga cgccatgtcg
     5401 gtatttatat cggcaacaat cagtttgtcc atgcttccac cagcagtggt gttattattt
     5461 ccagcatgaa tgaaccgtac tggaagaagc gttacaacga agcacgccgg gttctcagcc
     5521 gcagctaata aaccgtttgg atgcaatccc ttggctatcc tgacgagtta actgaaagca
     5581 ctgcttaggc agtgcttttt tgttttcatt catcagagaa aatgatgttt ccgcgtcttg
     5641 atccaggcta tagtccggtc attgttatct tttaaatgtt gtcgtaattt caggaaatta
     5701 acggaatcat gttcatacgc gctcccaatt ctggacgtaa gctcctgctt acctgcattg
     5761 tagcaggcgt gatgattgcg atactgggta gctgccttca gtttttagtg gcctggcata
     5821 agcacgaagt caaatacgac acactgatta tcgacgtaca aaagtatctc gatacctatt
     5881 ttgccgacct gaaatccact actgaccggc ttcagccgct gaccttagat acctgccagc
     5941 aggctaaccc cgaactgacc gctcgcgcgg cgtttagcat gaatgtccgt acgtttgtgc
     6001 tggtgaaaga taaaaaaaca ttctgttcat ctgcgactgg tgagatggac attccactca
     6061 atgaattgat tccggcgctc gacattaata aaaacgtcga tatggcgatc ttacccggca
     6121 cgccgatggt gccgaacaaa cccgcaatcg tcatctggta tcgcaaccct ttgctgaaaa
     6181 atagcggtgt gtttgccgct ctgaatctca acctgacgcc ttctctcttt tatagttcac
     6241 ggcaggaaga ttacgatggc gtcgccctca ttattggcaa tactgcgcta tctacctttt
     6301 cttcacgttt gatgaacgtt aatgaattaa ccgacatgcc ggtccgtgaa actaaaattg
     6361 cgggcattcc tctgaccgtt cggctttatg cggatgactg gacatggaac gatgtgtggt
     6421 acgcattttt actgggcggc atgagtggaa ctgtcgttgg cctgctttgc tattacctga
     6481 tgagcgtacg tatgcgcccc ggcagagaaa tcatgaccgc catcaagcgc gaacaatttt
     6541 acgtggcgta tcaaccggtg gtggatacac aagctttgcg ggtaacgggc ctggaagtac
     6601 tgctacgctg gcggcatcct gtagcgggag aaattccccc ggatgccttc attaactttg
     6661 ccgaatcgca aaagatgatt gtgccgctga ctcagcacct gtttgagtta attgcccgcg
     6721 atgccgcaga attagaaaaa gtactgccgg taggcgtcaa atttggcatt aacattgcgc
     6781 cggaccatct gcacagcgaa agctttaaag cagatatcca gaaactgctc acttccctgc
     6841 ccgcacacca tttccagatt gtgctggaaa ttaccgagcg cgatatgctg aaagagcaag
     6901 aagccacaca actcttcgcc tggctgcact cggtcggcgt agaaattgct attgatgact
     6961 tcggcaccgg gcacagcgcg cttatctatc ttgagcgttt tacgctcgat tatctgaaaa
     7021 ttgaccgtgg atttatcaac gccatcggta cggaaacgat cacttcaccc gtacttgacg
     7081 cggtgctgac gctggcgaaa cgtctcaata tgctgacagt tactgaaggg gtcgaaacgc
     7141 cggaacaagc gcgatggcta agcgaacgcg gcgttaattt catgcaaggc tactggatta
     7201 gtcacccgtt accgctggac gattttgttc gctggctgaa gaaaccgtat acgccgcagt
     7261 ggtaaggtgt gcttacgtcc cttattattc atagtgaaag catgccggat tgcggctaat
     7321 gatgagtaaa aggaaatccg ttgcagatga ttgtgcgcat actgctgctg tttatcgctc
     7381 tgttcacctt cggtgcgcag gcgcaggcta tcaaggaaag ctataccttt gccgtgctgg
     7441 gcgaaccccg gtacgcgttt aatttcaacc attttgatta tgtgaacccc gccgcgccaa
     7501 aaggtgggca gataacgttg tcagccctcg gtaccttcga taatttcaac cgctatgcac
     7561 tgcgcggcaa cccgggcgca cgcaccgagc agctgtacga cacgttattt acgacttccg
     7621 atgacgaacc aggcagttat tacccgctga ttgctgaaag tgcacgctat gctgacgatt
     7681 attcctgggt ggaggtcgct attaatccgc gcgcccgttt tcatgatggt tctcccatta
     7741 ctgcccgcga tgtagagttt acttttcaaa aatttctgac cgaaggcgtg ccgcaatttc
     7801 gtctggtcta caaaggcacc accgtcaaag ccattgcgcc gttaaccgtg cgcattgagt
     7861 tagctaaacc cggcaaagaa gatatgttga gtctgttttc gctgccggta tttccagaaa
     7921 agtactggaa ggatcacaaa cttagcgacc cgctcgccac gcctccgctt gccagtggtc
     7981 cgtaccacgt tacgtcctgg aaaatggggc aaaatattgt ctattcccgc gtgaaagatt
     8041 actgggcagc aaacttaccg gtaaaccgtg gacgctggaa tttcgacacc attcgctacg
     8101 attattacct cgatgataat gtcgcctttg aagcgtttaa agcaggtgcc tttgatttgc
     8161 gtatggaaaa cgacgctaaa aactgggcca cgcgttatac cggtaaaaat ttcgataaaa
     8221 aatacatcat caaagatgag caaaagaacg aatcagccca ggatacgcgc tggctggcgt
     8281 ttaatatcca acgtgcggta ttcagcgatc gccgggtgcg ggaagcaatc actctcgcct
     8341 ttgactttga atggatgaac aaggcgttgt tttacaacgc ctggagtcgc acgaacagtt
     8401 attttcaaaa taccgaatac gcggccagaa attaccccga cgccgcggag ctggtgcttc
     8461 tggcaccaat gaaaaaagat ctaccgccag aagtcttcac acaaatctac cagccgccgg
     8521 tatctaaagg cgatggctac gatcgtgaca acctgttaaa agccgacaaa cttctcaacg
     8581 aagcgggctg ggtgctgaag ggtcagcaac gcgttaatgc cacaacgggt cagccgctca
     8641 gctttgaatt attgcttccc gcaagcagca atagtcagtg ggtattgccg ttccagcaca
     8701 gcctgcaacg gctgggtatc aatatggaca ttcgcaaggt ggataactcg caaatcacta
     8761 accgcatgcg caatcgcgac tatgacatga tgccgcgcct atggcgggcg atgccgtggc
     8821 ccagttccga tttacagatt tcctggtcat cggaatatat caattccact tataatgccc
     8881 ccggcgtgca aagcccggtt atcgactcac tgatcaacca aattattgcc gcgcagggaa
     8941 ataaagaaaa attactgccg ttggggcgag cactggatcg cgtattaacg tggaattatt
     9001 acatgctgcc aatgtggtac atggcggaag accgtctcgc ctggtgggat aaattctccc
     9061 aacccgctgt acgccctgtt tacagcctgg gtatcgatac ctggtggtat gacgttaata
     9121 aagcggccaa actgccgtca gccagacaac agggagagta gatgggcgct tacctgattc
     9181 gccgtctgtt gctggtgatc ccaacgttat gggctattat cactatcaac tttttcatcg
     9241 tgcaaattgc gcctggcggt ccggtcgatc aggccatcgc cgccattgag tttggtaatg
     9301 ccggagtatt acccggcgca ggcggtgaag gtgttcgtgc cagccatgcg caaacgggtg
     9361 tcggcaatat cagcgacagt aattaccgtg gcggacgcgg attagatcca gaagtgatcg
     9421 ctgagatcac tcatcgctac ggtttcgata agccgatcca cgaacgttac ttcaaaatgc
     9481 tctgggacta catccgcttt gattttggtg atagcctgtt tcgcagcgcc tcggtgctga
     9541 cgctgattaa agacagtctg ccggtttcca tcaccctcgg attgtggagc acgctgatta
     9601 tctatctggt gtcgattccg ttaggcattc gcaaagctgt ttataatggg agtcgctttg
     9661 acgtctggag tagcacattt atcatcatcg gctacgccat tccggccttt ttgtttgcca
     9721 tcctgctgat tgtcttcttc gcgggcggca gctatttcga cctgttccct ctacgcggcc
     9781 tggtttccgc taactttgat tcgctgccgt ggtatcagaa aatcaccgat tatctgtggc
     9841 atatcacgct accggtgctg gcgacagtga ttggtggctt tgcggcgctg accatgctga
     9901 ccaaaaactc attccttgat gaagtgcgca agcaatacgt ggtgaccgcg cgtgcgaaag
     9961 gggtaagtga gaaaaacatt ctctggaaac atatgttccg caacgccatg ctgctggtga
    10021 ttgccggttt tccggcgacg tttatcagca tgttttttac cggctcgctg ctgattgagg
    10081 tgatgttttc actcaatggt cttggcttac tgggctacga agcgaccgtc tcgcgcgatt
    10141 atcctgtaat gtttggtacc ttgtatattt tcaccctgat tggcctgctg ctgaatattg
    10201 tcagtgatat cagctatacg ctggttgatc cgcgtataga ttttgaggga cgttaatgtc
    10261 gcgactcagc cccgtcaatc aggcccgttg ggcgcgtttt cgtcataacc gtcgcggcta
    10321 ctggtcgtta tggattttcc tcgttttgtt tggtttgagt ttgtgttctg aacttatcgc
    10381 taacgataaa ccgttgctgg tgcgttatga cggcagttgg tatttcccgt tgttgaaaaa
    10441 ctacagcgaa agcgattttg gcggcccgct ggcaagtcag gctgattatc aggacccgtg
    10501 gctgaaacaa cggctggaaa ataacggctg ggtactgtgg gcaccgattc gctttggtgc
    10561 taccagtatc aactttgcta ccgataagcc cttcccttct ccaccctccc ggcaaaactg
    10621 gctgggaacg gatgcaaacg gcggcgatgt gctggcacgc attctctatg gcacgcggat
    10681 ctcggttctg tttggcctga tgctgactct ctgttccagc gtgatgggcg tgctggcggg
    10741 ggcgctacaa ggctattacg gcggtaaagt cgatctctgg gggcaacgct ttattgaagt
    10801 atggtcgggg atgccgacgc tgtttttgat tattttactt tccagcgtcg tacagcctaa
    10861 cttctggtgg ctgctggcaa ttactgtctt gtttggctgg atgagtctgg tcggcgtggt
    10921 gcgggcggag tttttacgta ctcgtaattt cgactacatt cgtgcggcac aggcgcttgg
    10981 cgtcagcgat cgcagtatca tcctgcgtca tatgttgcct aatgccatgg tcgcgaccct
    11041 caccttttta ccgtttattt tatgtagttc gataaccacc ctgacctcgc tcgatttcct
    11101 cggcttcggt ctaccgctcg gttcaccgtc actcggtgaa ctgctgttac aagggaaaaa
    11161 taaccttcag gccccgtggc ttgggatcac cgccttcttg tcggtggcga tattattgtc
    11221 tttgctgatc tttattggtg aagccgtccg cgacgcattt gatcctaata aggcggtgta
    11281 gcatgacgca aactctgtta gcgattgaaa atttgtcggt gggttttcgc catcagcaaa
    11341 ccgtacgtac agtagtcaat gatgtttcac tacagattga ggctggcgaa acgctggcgc
    11401 tggtgggtga gtcaggttca ggcaaaagcg ttaccgcgct gtcaatttta cgcctgctcc
    11461 cttccccgcc ggttgaatat ctctccggcg atattcgttt tcatggcgaa tcgctgcttc
    11521 atgccagcga tcaaacgttg cgcggtgtac gcggtaataa gatcgccatg atttttcagg
    11581 aaccgatggt gtcgttaaat ccattgcata ccctggaaaa acagctttat gaagtgcttt
    11641 cactccaccg cgggatgcgt cgggaagcgg ctcgtggcga aattcttaac tgccttgatc
    11701 gcgtcggtat ccgccaggcg gcaaaacggc tgacagatta tccgcatcag ctctccggcg
    11761 gcgaacgcca gcgggtgatg attgcgatgg cgctgttaac gcgaccggaa ttattaattg
    11821 ccgatgaacc gaccaccgca ctggacgtct ctgtccaggc gcagatttta cagctgttgc
    11881 gcgaactgca aggcgagctg aatatgggca tgctgtttat tactcataac ctcagcattg
    11941 tcagaaaact ggcccaccgc gtggcggtaa tgcaaaacgg tcgctgtgtc gagcaaaata
    12001 acgccgctac gctatttgcc tcacccactc atccttacac acaaaagcta ctcaacagtg
    12061 aaccgtcagg cgatccggta ccgttgccag aacctgcctc aacgttgctg gatgttgaac
    12121 agcttcaggt tgccttcccc attcgcaaag ggattttgaa gcgcattgtg gatcataatg
    12181 tggtggtgaa aaacatcagt tttacgctac gggcgggtga aacactgggt ttagtgggcg
    12241 agtctggttc cgggaaaagc acgacgggac tggcgctgct gcgactgatt acctctcagg
    12301 gcagcatcgt ctttgacggt cagccactgc aagatttaaa tcgccgccag ctgttaccta
    12361 ctcgtcatcg tattcaggtg gtatttcagg atccaaactc ctcgctcaac ccacgactca
    12421 acgttttgca gattattgag gaaggcttac gggttcacca gccgacgctt tctgccgcac
    12481 aacgcgaaca acaagtgata gccgtgatgc atgaagtggg attagatcct gaaacacgcc
    12541 accgttatcc ggcggagttc tctggtggtc agcgacaacg tattgcgatt gccagggcat
    12601 taattcttaa gccctcgctg atcatacttg atgaaccgac atcatcactc gacaaaacgg
    12661 tacaggcgca aatattgacg cttttgaaat cattgcaaca aaagcatcaa ctggcctatt
    12721 tgtttatcag ccacgatttg cacgttgtcc gcgcgttatg tcatcaggtt atcgtactgc
    12781 gacaagggga agtagtggaa caaggaccgt gcgcgcgcgt gtttgccaca ccgcagcagg
    12841 agtatacgcg tcagttactg gcgttgagct gacgcttaaa aaggattgta gtttgaaaag
    12901 ggttcggcaa ttgccacacc aaaatttttc aatcggcatg ttgcagcaaa ctcgtcctgg
    12961 cgatttacaa acaggcacgg ctcaccttca cactccaaca ccgaacatgg aacttcgatg
    13021 tcgcttaacg cctggcttag tttatacatc accgaccatg cattatcacc atccgggcta
    13081 agcaatttaa gcgccaccag gctgttgtca ccgcacggtc cattttgcgg aatcggttca
    13141 accttcgaac ctgcgaaacc agcagaccag cgatagttct gcggcagtcg atggacgatt
    13201 gagagttgta atgaagtcac ttattttccc cggaagtaca ttcacttcac aatttgttta
    13261 catcaatttt aacacatcat caacaatccg tctttataca gatcgtaaag attgatgcct
    13321 cgcctgcgtg gcctctatgg ctctgattta agtaatatct gcgggcgtgg tcacgtttgc
    13381 ggggctatat gtacccgttt ctgcgatcta actcaacctt tttaactaca atgatgtgac
    13441 tttttacata aattgatttt acataaaata aacatatatc ggggaaatga tagatttgtg
    13501 gttgacatgc atcaacaaag gaagcctttt accttcctcg ttgtgcaata gatcaccgtt
    13561 ttttcggccg actggcgtac agacagaaga gaatggagct ggttgcgcag aatgcaattg
    13621 accaaatcat cggccatgca gagttaaagg tcgcaagaga aagcaatgcg ccaacaattg
    13681 cccctatgcc aaaacggaag gttcctgcca gcgaagatgc cgttcccgcc atatggggaa
    13741 actcatcaag aatgaccgcc atcgcattgg atgacaccat cgacacgcag cccacaaacg
    13801 ccgcaacgcc aaccaccagc gaccagaatc ccagccccag cggcgcactg atgaccatcc
    13861 acgcagccat aataaattgt atccacaacc ccgagcggaa catatttaac gcgccaatgc
    13921 ggcggacgaa gcggctgtta aagatggtca tcacgaacag aaaaacaatg tttagcgcaa
    13981 agtaataacc aaagttttcc ggcgcgacgt ggttaatttc aatataaaca aacggtccgg
    14041 cgcttaagaa tgagaacatc ccggcaaagc tgaaaccact ggcaagcatg tagctcagga
    14101 cacgtttatg gcggaacagc gccgcaaagt taccaatagt ggtacgaatg taaaatggct
    14161 gacgacgctc cggtggtaag gtttctttaa tcaggaagaa aatcattgcc gaagccagaa
    14221 tcgccgctaa tgccaggatc cagaagatgt aatgccagct cagccacacc agcacccagc
    14281 cgccaactat cggtgccatc agcagtgcaa tggtcgtcac cagcatgaca aacgacatca
    14341 tccgcgagaa ctcttctttc gggtaaatat cacgcatcaa agcgttgata accacgctgg
    14401 ccgcagccgc agccagcccg tggaagaaac gcatcacaat cagctgatcg atggtttgtg
    14461 ccaacgcaca cgccaccgcg gcggcggcaa acaccagcgt cccgccaaga accaccggct
    14521 tacgcccgaa gctgtctgcc atcggcccgt agattaactg ccccaacgca aagcccagaa
    14581 tataagtact gagggtcatc tgcgtactgc ccgccgatac gccaaactgc gctgaaatta
    14641 ccggtagcgc gggcagatac atatcaatcg acagcggcat caacatggcc agcaggccaa
    14701 ggataaaaac aatagcaaac gacgaatgct gtcgggtggt cacaacgggc tcctgaaagt
    14761 cattgaaaag ttagacgacg ctggcaattt cttcttcagt taacggacga tattcaccgg
    14821 gggctaaatc agcatccagc gtaataccgc caatacgttc acgatgcagc tcaaccacgt
    14881 ggttacccac ggcggcgaac atgcgtttca cctgatgata acgcccttcg ctgatggtca
    14941 gacgaacctg cgttggggta atcacttcaa gcaccgccgg cttagtgaga tctttttcgt
    15001 tatgcagctg cacgccttta gcaaattgct ctgccgtatc gtcagctaca ggtgattcca
    15061 gtgtcaccag ataggtcttc tcgcaatgat ggcgcggaga agtaatgcgg tgcgaccact
    15121 gaccatcatc agtcatcagc accagaccgg tggtatcgat atccagacgt cctgcggcgt
    15181 gcagtttcca cgctaccggt tcatccagaa aatagagcac cgtcgggtga tccgggtcgt
    15241 ccgtggagca aacatagccc tgaggcttat tgagcatgaa gtaacgtgga ccgtgttgct
    15301 gcgccagcgg gttgccatcg taagcgacat catgttcagg gagcagtttg aacgctgcat
    15361 tacgaacgat ttcgccatcg acggtgacac gattgccgcg gatttcacgc ccggcaatag
    15421 cacggctaac gccgagttgc tgtgcgataa atttatcaag tcgcatgtgt gtgattttgc
    15481 ctgtaaaaaa cggaggtcgg gtaatgaccc gaaaatctga actgttgtct gcccagtata
    15541 gcggtctaat aacttccctc aagggaaaaa gaaccgtggc atactattag cagaataatc
    15601 tacctacgcg aaaccatgat ttttacactc cgcccatatc agcaagaagc cgtggatgcc
    15661 acgctcaacc attttcgtcg tcataaaacc cctgccgtta tcgtgctgcc caccggcgca
    15721 ggtaaaagcc tggtgatagc ggaactggca cggctggcgc gtggtcgcgt actggtgctg
    15781 gcacacgtta aagaactggt ggcgcaaaac catgcaaagt atcaggcgct ggggctggaa
    15841 gccgatattt ttgccgccgg gctaaagcgc aaagagagcc acggtaaagt ggtatttggc
    15901 agcgttcagt cggtcgcccg taatcttgat gcctttcagg gtgaattttc gctgttgatt
    15961 gtcgatgaat gccaccgtat tggtgacgat gaagagagcc agtatcagca aatcctcact
    16021 cacctgacta aagtgaatcc ccacttacgc ctgctggggc tgactgccac gccttttcga
    16081 ttgggcaaag gctggatcta ccagttccat tatcacggca tggtacgcgg cgatgagaaa
    16141 gcccttttcc gtgactgcat ttatgagctg ccgctgcgtt atatgattaa acacggctat
    16201 ctgacgccgc cagaacgact ggatatgcca gtagtgcaat acgatttcag ccgcttgcag
    16261 gcacagagta acgggctgtt cagcgaagct gatctcaacc gtgagctgaa aaaacagcaa
    16321 cgcattacgc cacatatcat cagccagatc atggagttcg ccgaaaagcg caaaggggta
    16381 atgattttcg ccgccaccgt tgaacacgca aaagagattg tgggattact gcctgccgaa
    16441 gatgctgcac tgattactgg cgacaacccc ggcgctgagc gcgatgtgtt aattgaagat
    16501 tttaaagccc agcgttttcg ctatctggtc aacgtcgcag tactgaccac cggatttgac
    16561 gccccgcacg tcgatcttat cgccattctg cgccctactg aatcagtgag tctttaccaa
    16621 caaattgtcg ggcgcggtct gcgtctcgct ccgggcaaga ctgattgttt aattcttgat
    16681 tatgcgggta atcctcacga tctctacgcg ccggaagttg gtacaccgaa aggcaaaagt
    16741 gacaacgttc cggtacaggt tttctgccct gcctgcggtt ttgccaacac cttttggggg
    16801 aaaacgaccg ccgacgggac attgattgaa cactttggtc gccgctgtca gggatggttt
    16861 gaagatgacg acggtcatcg cgaacagtgt gacttccgtt tccgttttaa aaattgcccg
    16921 caatgtaacg cagaaaatga tattgccgct cgccgctgcc gcgaatgtga cacggtactg
    16981 gttgatccgg atgatatgtt aaaagcggcg ctacgactga aagatgcgct ggtattacgc
    17041 tgtagcggca tgtctttgca gcatgggcac gacgagaaag gcgaatggtt gaaaatcacc
    17101 tattacgatg aagacggcgc ggatgtgagt gagcgtttcc gtctgcaaac gcccgcccag
    17161 cgtaccgcct tcgagcagct ttttatccgc ccgcatacgc gcacaccggg catccctctg
    17221 cgctggatca ccgccgccga tatcctcgcc cagcaagcct tactgcgaca tccggatttt
    17281 gtcgtcgccc gcatgaaagg tcagtactgg caagtgcgtg aaaaagtgtt cgattacgaa
    17341 ggtcgttttc gtcgggcgca cgaattacgc ggttaatggt agttttgatt gatgtataag
    17401 gcgattgagt atagaatctc gcccgctttt gcatacgcaa agcagatcac ttacctgttg
    17461 ctgggtcgcc tgtagcagga ttaatttaag agagaaagaa atgtttacta tcaacgcaga
    17521 agtacgtaaa gagcagggta agggtgcgag ccgccgcctg cgtgccgcta acaagttccc
    17581 ggcaatcatc tacggtggca aagaagcacc gctggctatc gagctggatc acgacaaagt
    17641 catgaacatg caagctaaag ctgaattcta cagcgaagtt ctaaccatcg ttgttgacgg
    17701 taaagaaatc aaagttaaag ctcaggacgt acagcgtcac ccgtacaaac cgaagctgca
    17761 gcacatcgac ttcgttcgcg cttaattgct gaataagttg taaaaaaccc cgctccggcg
    17821 gggttttttg tatctgcaga ttatgcctga ttacggtatt gctatttttt gcaggccaga
    17881 taaggcgttc acgccgcatc tggcattcag aacacaaaac cgattaattg ccgccagacg
    17941 tccggcgctg caattggtcg cgcaaattcg gcggcgtgcc tttaatggtc aaggtatcgg
    18001 ttgctggatc ccagaagatg cgttcaccta acagcatcgc atcaaagtta atagtcaagc
    18061 caccgccgct accggcaaac ttcgtcaact gacgcagtgt gctacggtct gccgggaagc
    18121 tctcttccag ttcgtagcct ttttcagccg caaactccgt gaagctcact tcgcttacgc
    18181 ctgccagctc tttcgacagc gattccagtt cgatctcttc gcctgcctgc agctgctcat
    18241 tacagtagct ataaacctgc tggcgcacgt tctgtcgctc tgctttatcc agttgtgctt
    18301 cggcagtgaa atcatcaacc gcttgcaaca gcccacggtt ttgcgctttc gcgttcaatc
    18361 cttcgctggc cccgaggaaa tccataaaga aatccgccac tttgcgccct actcgccctt
    18421 tcaggaaagt gagatagcgg gtggactctg gattggtttc ccattcggtt aaatcgatac
    18481 gcgcaacaat atccgcatga ttgatatcaa gataatgggt tgggttgatg tcgagatttt
    18541 cgttaacgcg catactgctc aggttgctca gaactgccac cagcaaatac tccaccgcca
    18601 gataacggta gtggcaaaac agcacgaatc caccgtcagc aaaaggatat ttcgctaatt
    18661 cgtcacgcag gcgaccggtt gccgcgcggc taaatgccag aaagtcctct tcgccctgac
    18721 gctgtaagcg cagcgtctgc gccagttcac tctcttcgct aaacaagccg taagctttat
    18781 ttttggcgct atagacccga tgcagttctg ccaccatctc aacgacggtt tctgtcggtt
    18841 ccagcaatga atcgcgcaac accagctcaa ggttctgctc atcacgcttg ataagctggt
    18901 gcagagcaat ctggttgata tccagactca tgataaactc tcctttaaga ccgggcggta
    18961 ttcaaccacc gccagttaag cgacgcaata aaagatggcg gtcattttac agaaggcagc
    19021 gtaacgagaa tgatttaacg cataaaaaag cagaaaaaaa accgttgcta cggtaatatg
    19081 ttgccctttc atcaactaac tgatttcgat ttatgccaca aatttcccgc tacagtaatg
    19141 aacaggttga acaattgctc gcagagctgc tcaacgtact ggaaaaacat aaggctccga
    19201 ccgacctttc cctgatggtg ttaggcaaca tggtcactaa ccttatcaac accagcattg
    19261 ccccggccca acgccaggca attgccaact cttttgccag cgccttacag tcctctatca
    19321 acgaagacaa agcgcactaa gggaaacaga taacaggtta tggtaactca tcgtcagcgc
    19381 taccgtgaaa aagtctccca gatggtcagt tgggggcact ggtttgcact gttcaatatt
    19441 ctgctttcgc tcgtcattgg cagccgttac ctgtttatcg ccgactggcc gacaacgctt
    19501 gctggtcgca tttattccta cgtaagcatt atcggccatt tcagcttcct agtgttcgcc
    19561 acctacttgc tgatcctctt cccgctgacc tttatcgtcg gctcccagag gctgatgagg
    19621 tttttgtccg tcattctggc gacggcggga atgacgctat tactgatcga tagcgaagtc
    19681 tttactcgtt tccatctcca tcttaatccc atcgtctggc agttggttat caacccagac
    19741 gaaaatgaga tggcgcgcga ctggcagctg atgttcatca gcgtgccggt tattttattg
    19801 cttgaactgg tgtttgcgac gtggagctgg caaaagctgc gcagcctgac gcgtcgtcga
    19861 cgcttcgcgc gcccgctggc cgcattctta tttatcgcct ttatcgcctc gcatgtggtg
    19921 tatatctggg ccgatgccaa cttctatcgc ccgatcacca tgcagcgcgc taacctgccg
    19981 ctttcgtacc cgatgacggc gcgacgtttc cttgagaagc atggcctgct tgatgcgcag
    20041 gagtatcaac gccgtcttat tgagcaaggt aatccagacg ccgtttccgt acagtatccg
    20101 ttaagcgaac tgcgctatcg cgatatgggc accgggcaga atgtgctgtt gattactgtc
    20161 gatggcctga actactcacg cttcgagaag cagatgcctg cgctggcagg ttttgctgag
    20221 caaaatattt cgttcacgcg ccatatgagc tccggcaaca ctacagacaa cggcatcttt
    20281 ggcctgttct atggcatctc gccgagctat atggacggca ttctgtcgac ccgtacgcct
    20341 gcggcattaa ttactgcgct taatcagcaa ggctatcagc tggggttatt ctcatcagat
    20401 ggctttacca gcccgctgta tcgccaggca ttgttgtcag atttctcgat gccgagcgta
    20461 cgcacccaat ctgacgatca gaccgccacg cagtggatca actggctggg ccgctacgca
    20521 caagaagata accgctggtt ctcgtgggtt tctttcaatg gcactaacat tgacgacagc
    20581 aatcagcagg catttgcacg gaaatatagc cgggcggcag gcaatgtcga tgaccagatc
    20641 aaacgcgtgc tcaatgcact gcgtgattct ggcaaactgg acaatacggt ggtgattatc
    20701 actgccggtc ggggtattcc actgagcgaa gaggaagaaa cctttgactg gtcccacggt
    20761 catctgcagg tgccattagt gattcactgg ccaggcacgc cggcgcagcg tattaatgct
    20821 ctgactgatc ataccgatct gatgacgacg ctgatgcaac gcctgctgca tgtcagcaca
    20881 cctgccagcg aatattcgca aggtcaggat ttgttcaacc ctcaacgccg tcattactgg
    20941 gttactgctg cggataacga tacgctggca attaccaccc cgaaaaagac gctggtgctg
    21001 aacaataacg gtaaataccg cacttacaac ttacgtggtg aaagagtgaa agatgaaaaa
    21061 ccacagttaa gtttgttatt gcaagtgctg acagacgaga agcgttttat cgctaactga
    21121 ttaattataa atcagttagc gaaatatctt acttgcaatc ggtgtggaaa acggtagtat
    21181 tagcagccac gagtcggcac gtagcgcagc ctggtagcgc accgtcatgg ggtgtcgggg
    21241 gtcggaggtt caaatcctct cgtgccgacc aaaaatccca agaaaaaacc aacccttacg
    21301 gttggttttt ttatatctgc aattaattcg ataaacagac cgtgacacat cacagcctgt
    21361 ttattttctg ttatcagaac gtccagacca cacccgcctg agtattccac ggcgattcta
    21421 cccctgcgcc attgccatag ctgactgaca aatgaccgct taacgtcggg gtaaacgatg
    21481 accttatccc agcctgataa acgccactgg ttccagccac atcattatta aatttgccat
    21541 cgtcattaac tttcacctgg ttagaatcgg cgtattcctg acgcacggcg gctttcagcc
    21601 agggttccag cgtcgtaccg ttttgcaggt ccatgtgata gcttaccgcc gttcccgctt
    21661 cagcgcgtaa tatccgggta tttcccacat ccgcgcgcat gccgtttgat aacgtgtagt
    21721 cctgaccatc tgtggtaaag ccggtaaagg ccagataggg tctaacactc cacaatccgt
    21781 caacccaacg gaacccgctc tcaacatgag cacccgcgcc gttactattg taatcgccaa
    21841 acgctgttgc cccattactc atcttgccat ggatggtgtt ggcaaaacgg tcaactttca
    21901 ccaccccatc aacataggca ccgttctgat gctcccaacc ggcataagcc cccagggtat
    21961 agctatcgat attacctttt ccgccgcgat caaaaccaat atcagaatga gagtaaccaa
    22021 agaacaagcc gcgaattgta ctgctttctt cacgggagaa acggctatcg ataccgagcg
    22081 tcaggcccgt caatgtttgc tcaaaaccag ctcccgcatc agtggtcacg ttgttgcggg
    22141 tgttaattgc cgaactccac atcaccgtat cgtaactaac gccttttacg ctaccaagac
    22201 gctcacgcac ggtgtccagt tctgcatcaa ataccagcgg ttgtgcggct gccatgttca
    22261 gcacatcagt ggttgaaggg gtaatttgcg cgcgattctc tgcgaggctc cagctatggt
    22321 tgccattatc cagcaaggta tattcatacg taccgatatc aacaacgcct ccggcattgc
    22381 ccaacgtaaa tgcagcatca ccgccgcccg ttgttaccag tgtaaggcta tctcctgctg
    22441 ccgggctggc accggtgtcc gtcacgaata ttttgaaatc acctgttgcc tgaccggtga
    22501 cgttgagctg atcgctctga tgattggcca tatcggtacg catataaaaa ttgccgtttc
    22561 ccgacagggt attggtggtc aacgtaatga aattttccgc acttgccgtc acagatgaac
    22621 gagtcactgg ctcagccatc ataatcacat ttgcgccgga catcgcaagg ttggctaagt
    22681 tgaggcgagt gttttggttc gtcgtagtga tatcggcaat gatggtccat gcgctatttt
    22741 cgagcgaaac gtccgctttg ctggtatcca cggcaccatg cgttctaaaa gaagcgccct
    22801 cagaaaccac taccttaccc accaacatag cgggtgcata atttatttgc ggcttcatga
    22861 cctcaagaat gccatcattc cctctgactg acacgttaac cattgtgcca gcccagacat
    22921 tagcgcggcc attgttgatt accatatttt cagagatggc ttttgaggaa tacgtgatac
    22981 ttccattctg gtctgatcga ccgagatcat ataccgcatt tgcctgcact ttcgtaccag
    23041 tatcctttcc cagcgattgc atagtggcat gctcatcaag gatagtgtca atggcctgcg
    23101 tgtcctccat aacaatgagc ccggaaccat catccagttc ataatttttt gacacaccat
    23161 cctttatact aaattggcct ttactgtttg taccactcac ttccagcgca ttcgttgaga
    23221 caattaattt cccgcctgct tttttatcaa cgccagttgc agtcccgcca tccataacct
    23281 ccagtaagcc gccactatcc acagtagtat tataagcgaa gtcattttct tcaactcgca
    23341 aacttccgcc gttttccaac aacatattat tagcaatacc attcttgata tcgaactgac
    23401 cgagacggtt tgttccgaat acctccatgg cccgcgtggt tgttttaata gcaccgcctg
    23461 ctttctgatc taccgcaagc gcaaatcccc cctctttaac ctccagtagt cctccctttt
    23521 cgatagcggc aaaaacggtc cccccatctt tttcaacgat taagcgcccg cctttaatcg
    23581 tggtatcatt cgctgggcca taaacctgta gcacaccatt attgagagta gtctggtttg
    23641 ccgcgccacc catttgaaca atttgataac catcatttaa tactgagtat tctgcggtac
    23701 cattcatttc aatgtattga taccctccgt taatttcagt attactactt acaccaaatt
    23761 cttttatatg ctgttctccg ccaggattga ttttcgtatt attggcggtc ccataaacca
    23821 gctggatatc tttttccagg ataacaccat caactgtctc accatactca actgttgatg
    23881 ccaatgctat gggtgaatat aacgctgtag ccaccagaac agatattgcg gaacgacaaa
    23941 gagaaacaga accagattga tgcattgagc tttcatccta tgaaattaat tgcagttaaa
    24001 agcattgggt acagaaaata cccatagctc catacccgga gtcagttttt aaaaactgtt
    24061 taaagaaatg cacaagtatt gtgattgatt ttttagttgt ttttcttgat gagaagctga
    24121 tgcaaaattc cgtctttata ataaaaatga tgccaaagcg aacgacaagg ttgtagtttt
    24181 cactacatgt ccatacataa aatggggtaa cattcacgcg cctggtagcg ttaccaacgc
    24241 tacgctcaaa cataatgatt ctaataaaac ctcaggagac tactatgcct gaagcaacgc
    24301 cttttcaggt gatgattgtg gatgatcatc cacttatgcg acgcggtgtt cgtcagttac
    24361 tggagcttga tcctggcttt gaagtggtcg ccgaagcggg cgaaggcgcg agcgcgatcg
    24421 atctggcgaa tagactggat atcgacgtga tcttgctgga tctcaatatg aaaggtatga
    24481 gtggcctgga taccctcaat gccttgcgca gggatggcgt taccgcgcaa attattatcc
    24541 tgaccgtatc cgatgcctcc agcgatgtct ttgcgctgat agacgcaggc gcagacggtt
    24601 atctgttgaa agacagcgac ccggaagtat tgctggaagc gattcgtacc ggagcgaaag
    24661 gcagcaaagt ctttagcgaa cgcgtcaatc agtacttacg tgaacgtgaa atgtttggcg
    24721 cggaagaaga tcccttcagc gtgctgacgg agcgcgaact ggatgttctg cacgagctgg
    24781 cacaggggct gtcaaataaa cagattgcat cggtgttgaa tatttccgag cagacagtaa
    24841 aagtacatat tcgcaatctg ctgcgtaaac tcaatgtccg ctcacgcgtg gcggccacca
    24901 ttctgttcct gcaacaacgc ggggcacaat aaaaatagcc cgatggattt atcatcgggc
    24961 tgagatttat gacaaacgca aaactgcctg atgcgctacg cttatcaggc ctacgtggat
    25021 cgatcaattt attgaattta cacaattttg taggccggat aaggcgttca cgccgcatcc
    25081 ggcataaact aagcgcactt tgtcatcagt ctagcccgat ggcatcacca tcgggcctct
    25141 ttttatttac tctcctgcgg cgacaaatgt tgcatcgcct gcgcgatact acgttcaatc
    25201 accgcacggc gagtatcgtt ggcaggtaag agtttcaaca tcatctccca cgcggcaacg
    25261 gcttcgccaa atcgctgctg ctcaaaggca ttaaacgcat acatgcttag cacacggata
    25321 ttgctatggt ccgttctcac cagctgacgt agcagttcac cgccgaggcg gttgtcgttg
    25381 ggatcagatg aacgagtcaa cgcttcagcg tatcccagtg cggcatcgct gttcttcgga
    25441 tcgaggcgat acgcagtagc gtatgcatcg gtggcgatac tggcgttacc cagcgccatg
    25501 ccaacgcggc ccaacataat ccagccttct atatctcccg gatttttttg cagttgagta
    25561 cgcatcccca gcgcaaggcg cgacatctct tcttcgttga gcggatcggc tttcgggtcc
    25621 agcgccctgt ccagcaacgc cggagcctgt gccgtggcct gctgccagat tttcacctgc
    25681 tgataattgc cagtctggta gtagctgacg ccagccacaa ttaacgccac cacaataccc
    25741 ggcagataaa caacatatcc ggcacgctta ccttccggca cgctttgttc aggaaacgct
    25801 tccggcacca cgcgtacccg acgccgcgag cgggcgtata tgacccaacc gccaatgcca
    25861 atagccacta ctggcagcac ccacagcagc acggtcagcg gcgttaacgg cggatcgtaa
    25921 gtgacgaagt tgccgtaacg cgccaccata taatcgacaa tctctttttt acttttacct
    25981 tcctgcatca gttcatacac tttctgacgc aggtcggtgg caatcatcga gttggaatcg
    26041 gcaatgctgt tgttctggca tttcgggcag cgcagttctt cagtgagctg acggaactgt
    26101 tgttcctgtg cttcatcttt aaactgcaac acgtcgatgg tcgccagcgc tgagccggag
    26161 atcatcagca tcagcacgcc caataaaaac ctcattgtgc ggcctcctta ctgtatttct
    26221 cccacagcgg cttgatcttt tcttcccaga cgcgaggatt gagatcgccc gcatggcgat
    26281 agcgaatgat accgttgccg tcaataagga aggtttctgg cgcgccatag acaccgagat
    26341 ccagccctaa catgccgtcg ccatcaaaca ggcttagcgc gtaaggattg cccagctctt
    26401 tcagccagct gattgccttc tggcgatcgt ctttatagtt catgccgacc acgcgaatgc
    26461 cctgcgctga aagctgattc agatattgat gttccgcacg gcaggtcgga caccaagtcg
    26521 cccagacgtt aagcagtact ggtttgccct gagtcagcac atccgcctga taaaactgcc
    26581 ccggattgtc cagtgattcg agacgaaact tcggcacagg cttgccaatg agcgccgatt
    26641 ccagattggt cggatcatcc ccttcggcat tacgcgccag ctgccacagc agcgccgcgg
    26701 caatcgccag gaagataatc aacggaatta acaatacttt gcgcttcata cggcctccgg
    26761 cgcagttttt tgcggactca cgcgcttacg atagcgagga tcaaacagac acagcagtcc
    26821 gcccaacgcc atcatcagcc cgcccgccca aatccagcga acaaatggtt tgtagtaaag
    26881 acgcacggcc cacgcgccgt tttccagctc ttcaccgagc gcggcgtaca ggtcacgcgt
    26941 gatgccgccg tcaattgccg cttcggtcat catcgacccg gcagtgttgt aataacgttt
    27001 ttccgcatac agcaccgttt ccggtttacc ctctcgcgtt acgccaatag tcgccacacc
    27061 gccgcgccag ttcgggccag tcacctcttt gacatcacgg aaggtgaagc gatattcatg
    27121 aatatcgacg ctatcgccgg acttcatgcg cacatcacgc tcaacgctat aattctggct
    27181 aaaggcaatg ccaacaattg tcactgccag ccccaggtga gccgccacca tcccccaata
    27241 actgaaggtg gttttcgtgc cgcgtgaaat acgcagcgca gcttccgcag ttgccagcac
    27301 cgcaatccag caggccattg ccaggccgag caccgtcatc gccacaactt tgctttcgaa
    27361 cagccacggc aacagcagag acagcaccag cgtagagatg aaggcgataa tcaataaatt
    27421 gcggatttta cgtgggcgat cgcgccccca gcgcaccaga ggaccgacac caagcagtag
    27481 cgcaaacggc accatcagcc aggtaaacat ggtgttgaag aacggttcgc caatcgaaat
    27541 actgcccagt ccaagttgct tgtgcaccag cggcagcagc gtccccagca acaccaccag
    27601 catcgcggcg accagcaaaa cgttgttcgc taacagcaaa gattcccgcg accacagcgc
    27661 attgtttacg cgtgagcgaa ctttgtgtcc acgcgcggca aacagcagca gcgaaccgcc
    27721 aatcaccagc accataaagg cgaggataaa cataccgcgc gccggatcag acgcaaacgc
    27781 gtgtaccgat accagcacgc cggaacgcac gaggaaagtc cccaacagac acaacgagaa
    27841 ggcactgatt gccagcagta atgtccacgc tttgaagctg gcgcgttgtt cagtgaccgc
    27901 cagtgagtgc atcagtgcag tccccaccag ccacggcata aacgaggcgt tttctaccgg
    27961 atcccagaac caccagccac cccagccgag ttcgtaatag gcccatgcgg aaccgagcac
    28021 aatgccgagc gtcaggaaaa tccacgccgc cagcgtccac gggcgagtaa aacgcgcata
    28081 agtgctgtcc agacgcccgc tcagcaaaga agcaatggca aaagcaaacg ccaccgagaa
    28141 acccacgtac cccatataaa gcagaggcgg atggaagatc agccccggat cctgtaacag
    28201 cgggttaaga tcgcgccctt caatcgggaa gttcggcagc gtgcgtgaga acgggttaga
    28261 ggtaaagaga atgaacagca aaaagccgac gctgaccatc cccattatcg ccagtacacg
    28321 ggccacaata tccagcggaa tacgctgact aaaaatcgcc accgcaaagg tccagccgct
    28381 catcagcagc acccacagca gaagcgaacc ttcatgcgcg ccccaagtag ccgccacgcg
    28441 ataccacacc ggaagctggg tattggagtt gctggcaaca taggtgacgg taaagtcgtt
    28501 gaccacgaaa gcattgacca gcaccagaaa tgcgccagcc acagacataa acagcagcca
    28561 ggcaaacaag cgggaagacg ccatcatgcg cgcatctccg cgcgccacgc cccatagcgg
    28621 atacacggac agcagcagcg caatccctaa cgccaagcac agcagcccgt ttccaatttc
    28681 cggcatcatg atgctgggtc cttataaaca ctcgccgggc gacggtggtt agcttccatc
    28741 gctttctcaa cttctggcgg cgtgtagttt tcgtcgtgtt tcgccagcac ttctttcgcg
    28801 aggatatgat tgcctttttc cagctcgccc tgcaccacaa cgccctgccc ttcacggaac
    28861 agatccggca aaatgccttc gtaagagaca tccactgagc cttcagcatc gtaaatggtg
    28921 aaggtcactt tcagcgaatt gggatcgcgc tgcacgctac ccggcatcac catcccgcca
    28981 acgcgcagac gctgaccgac ttccggcatc tgctgagttt cacgcttgcc gtagagaatt
    29041 tcccccggcg tataaaagag atcgatattc gagcgcagcg catatagcac cagaccgata
    29101 gtcagcgcta gccctgccaa cacggcacag gcaatccaca agcggttttt acggcgaata
    29161 ttcatgcagc ctcctgctgt tgcgcagcac gtaaacgcgc ctcacgcgcc cgctgttgcg
    29221 ccacaccacg cagaattgcg cgatgttgca tcaccgagtg cacgaccaaa accaccagcg
    29281 gaataacggt catcaccacc gccagccaga caaaaaaggc gtaaccgccc attgcgaaaa
    29341 attcattcca ggaagcaaat gcaggggtca tttacggcct cttttcagta tcagttcact
    29401 cacccacgga cggcgttttt ccatcagcaa aatcaaatta cgcatccgca tcagcgtcag
    29461 cgtggcagac aggagcagga agccaaaaat cgaccagcgc agcggcgaac gcatcgccgg
    29521 atcgatactt tgctgcatcc gcgttgatcc ctgatgcagg gtgttccacc actccacgga
    29581 gtaatgaata atcggcagat tcaccacgcc aatcagcacc aggatacctg ccgcacggcc
    29641 cgccagacgg cggtcgtcga aggcgtgcca cagggcaatc acacccacat acaaaaacag
    29701 cagcaccagt tcagaagtca gacgtgcatc ccatacccac caggtgcccc acatcggttt
    29761 tccccatgca gagccggtaa ccagggcaat aaaggtaaac acggcaccaa tgggggccat
    29821 cgccgccacc gccaggttgg ccattttcat ctgccagaca aggccaataa acgccgccac
    29881 cgccattgat gcataaatgc ccatcgacca gatcgccgca ggcacatgca ggtagataat
    29941 gcggtagcta tttccctgct gataatcagc cggagcaaag ccgaatcccc agatccagcc
    30001 gacggtaagc acgaccacac tggcaattgc cagccacggt ataaaccagc cacagatttg
    30061 atacagccgt ggtgggatcg ccagttgatg cagtgttttc cacatagttt cgataccaga
    30121 ctcgaacaaa aatcagtaat ccagcgttat tgaatgctga ttcgtaacgc tgccgccgtc
    30181 gcaaaaggac ttaatgtcgc ggtgcctgcc agcaacgcgc ctaaaattgc cagataccca
    30241 tcaacgggca aatgcataga agccgcgtcc atcgcggcgg tggcaaagat gagtaatggg
    30301 atagtcagcg gtaacaccag tatgctgagc agcacaccac cgcgcttaag tcccactgtc
    30361 agcgccacgc ccggtgcacc gagaaagcca agcgtaggcg ttcccagcag cagcgtcagc
    30421 gccatcactt gccagccata aacatccatt cccagtagca ttgctaccag tggcgaaagg
    30481 atgagtaacg gcagaccggt taccatccag tgcgccatca ccttcgccag cacaacggcg
    30541 ggcaagggta acggcaacaa catcaattgt tcaagactgc cgtcctgcaa atcgtcacgg
    30601 aacagtcgtt ccagcgccag caaggatgaa agcagcgcag caacccagat aatgcccggt
    30661 gcaatacgcg ccagcagttg cggctccgga ccgatactga gcggaaaaag ggtaattaca
    30721 atcaggaaga accacagcgg gttggcgatt tcggcgctat ggcgaaacgc tacgcgcagt
    30781 tcaagacgga aaatacgcca gaacatcatg cggccctcgt ttgcgtcagt gaaatgcggc
    30841 gaattttact ttcagcaacg ttgagcggct ggtgggtagt cagaatcaca atccccccct
    30901 gctccgtatg ctgcgccata cgctgggtca gacgatcgac gccgttaacg tcaatcgtgg
    30961 taaaaggctc gtcgaggatc cataatgtgg cacgggtcaa ccacagacgc gctaaagcga
    31021 cgcggcgttg ttgcccggca gaaagttgat ttaccggaat atcttcgaat ccggcaagcc
    31081 cggcctgcgc caaggcttcc agacattgtg cggcatcgcc atcgcgatga taaaagtgca
    31141 gattttctaa cgccgtcagc cgggttttga tccccggctg atggcctatc cataacaggg
    31201 tttgatggta gctgtcgcgt acctgatgca agggctgccc ttgccagaga acctcgccag
    31261 cgtcagggcg agacaacccc gtcagcaaac ggagaagcgt tgtcttcccc gcgccgttgc
    31321 taccggtgat ttgtacccac tctcctgcgt tcagcgtaaa tgacaagcca ctaaataagg
    31381 ttcgttcatc ccgctcacaa agtaactctc tggcttcaag catacccacg caataaccct
    31441 gttaaaaacc tggctcgact tcacgcatat cgggcagctt gtgcgcgatc cctttatggc
    31501 aatcaataca ggtttgccca tctttcaccg cctggtcatg catcttcgcg gcaaccgatt
    31561 tctgggcggt tgtatccata tactcgaagt tgtgacagtt acggcactcc tgcgagttat
    31621 tgtccttcat gcgccgccac tcattctgtg ccatcgtcag acgatgagct tcaaatttct
    31681 gcggcgtgtc aataacgcca aaaattttac catacagctc tttacttgct ttgagcttgc
    31741 gtatcatctt cggcacaaac tcgtgcggaa cgtgacaatc cggacaggtc gcacggacgc
    31801 cgctacggtt gttgtagtgc acggaatcca tgtattcctg atacaccgtg ttgcgcattt
    31861 cgtggcagct aatgcagaac tcttcggtat tggctttttc catcccggtg ttaaagcccc
    31921 cccagaagac gatgccgcca acaaaaccga tcaacagcag cgtccccagc gccagatggc
    31981 tgggggtacg ccaccatttc cacaggcgct taatcagacc aggcttacgg tcagaatttc
    32041 ccataataac ctcttatttc ccgtaacctt ttgatggggt aaaggtattc cccacgattg
    32101 gcgcggtatc ggcctgcggt acgtgacatt gcagacagaa ataacgacgc ggagccactt
    32161 ccgcgcccac tttgccgtcg ctgtccataa agtgagtagg actgatacgc ggcgcgccag
    32221 tggtgcgata gctttcgaca ccgtggcact gcaagcagcg attggtattg gtcgttacct
    32281 gataaccttc aacgctatgc gggatcattg gcggctgatt cacatagttc agcggcatcc
    32341 gatcctgctc ttttggcatc cgaatggccc cttcctgcgt cccggatact tccggcgact
    32401 ggctaaaatc gacgccatta gccgcccaaa ccgcgccgct taccaccagg gccagcatcg
    32461 ccgtccattg acacagcgct ttcttcaggt catggctttt catgatttcg ctcccgaact
    32521 ccatcgtgta gttattgtaa atacatcctc agaacagaca tccacgcagc gaccgcacgt
    32581 catgcaatcg cggctggtga cctgcaccgg gctttgttca tccagcaccg gggcacgtag
    32641 cacatgcggt tccgggcaaa catgaaaaca atccatacag cggttacatt tctgccgatc
    32701 gctggccgca acggtaatca cacctttgct acccagcacg ccatacagcg cgccaaccgg
    32761 acaaatgtgc ccgcaccagc cgtgttcaac gaccagtaga tcaaataaaa acagcgcgag
    32821 aataagcagc gcgccgctgc cgaagcccat taccaggcta cgccccatca gagaaacggg
    32881 gttgatccat tcccaaatga gcgtgccagt taaggctgat cccaccagaa tgaccaccaa
    32941 cagcacgtag cgaatgtggc gagggatcgt cgcagactga ttgaggtcaa agcgcctgcg
    33001 taaccagttc gctaagtcgg taatcgggtt cagcgggcag acccagctgc aaaataatcg
    33061 ctttcccgcc agggcgtaga gcacggtgat aatcaccgca ccggtcaacg ccaccgtggc
    33121 aggcagatga ccactggcaa gactttgcag cgtcatcagc ggatcggtca gcggaacggt
    33181 gtcgaataac aggctactgc tgtagttgcc gtgcaagatc cacacaccaa accatggacc
    33241 gctcaaaaac atccccagca cgaagaactg acaaaggcga cgtaacacca gccaacggtg
    33301 actgcgccac cagccttttt tctccagcgc ctcgcgcccg gcgtcacgtt tacgatttgc
    33361 cattgttccc ctccagccag ccgaagcggt aatggtgacc taactcccct ttcgccagtg
    33421 acagcggtaa caccttgatt gccggttgtt ccagcacgca tactttttcg cacttaccgc
    33481 aaccggtaca ggcgtcgctg tgaaccgtcg gcagaaagcg ggcgtgctta ccggtacgcg
    33541 tgttgcgctc cagctccaag gtgatggcct catcaatttt cgggcattcg cgataacaaa
    33601 catcgcagcg cagcccctga aagttgagac agttttcctg gtccaccagt accgccagcc
    33661 ccatccgcgc gtcgtcgatc gattcaatct cacgatccag cgcaccgctt gggcacactt
    33721 tggcgcacgg aatgtcctca cacatttcgc aaggaatatc ccgtgcgaca aaatatggcg
    33781 tgcccgccga cagaccagag gccagcgtcg ccagttttaa ggtgtcgtaa gggcaagcct
    33841 gaacacactg accgcaacgc acacaggcac tggcaaaggc gttctcgttt atggctccgg
    33901 gcgggcgcaa ccgcacgcca gatgcgcgtg cggtttgctg ttgtaacccc agcgccacac
    33961 ccacggcagc cagcccgcct gctgtgcgaa cgacatcgcg cagaaagcgg cggcgaccat
    34021 tttgaggttt cgctgaccgg gacataatgg cttacacctt ctccagtttg accgcgcact
    34081 tcttgaagtc cgtctctttc gagagcggat cggtcgcatc cagcgtcagt ttgttaacca
    34141 gctgtgcggc gtcgaagaac ggcatgtaca ccaggccctg tggcggacgg ttacgaccgc
    34201 gcgtttcaac aatcgagatc acttcgccac ggcgagaaac cactttcact ttgtcgccac
    34261 ggcgcagatc gcgcgctttc gcatccagcg ggtgaataaa caagaccgct tccgggaagg
    34321 cgcggtgcag ttccggcaca cggcgagtca tactgccggt gtgccagtgc tccagaacgc
    34381 gtccggtaga gagccacagg tcgtactctt catccggtgc ttccgccgcc ggttcgaacg
    34441 gcagtgcgaa gatcaccgct ttgccatccg gtttaccgta gaacttatag ccttcgcccg
    34501 ctttcacgta cgggtcgtta ccttcgctgt aacgccactg tgtttcttta ccattcacca
    34561 ccggccagcg cagaccgcgc gctttgtggt agtcatcgaa cggtgccaga tcgtgaccgt
    34621 gaccgcgacc aaaccatgcg tactcttcga acagcccttt ttgcagatag aagcccagct
    34681 cgcgggattc atcgttcagc tgatcttccg ccagttcgga taccgggaat ttgctcactt
    34741 cgggggtggc atacagaact tcgtacagcg ttttgccacg cagttccggt ttcttcgcca
    34801 gcagctcttc cggccatact tcttcagttt tgaagcggcg ggagaactgg actaactgcc
    34861 agagatccga tttcgcttcg cctggcgcct gtacctgctg acgccagaac tgagtacggc
    34921 gttcggcgtt accgtaagcg ccctctttct ctacccacat tgcggtcggc aggatcaagt
    34981 cggcggccag cgcactgact gtcggatacg gatcggagac gatgatgaag ttgcgcggat
    35041 cgcgccagcc cggcatacgc tcttcattga tgttcggccc ggcctgcatg ttgttggtac
    35101 acatggtcca gtaaacattc agcttgccgt ctttcagcgc acggtcttgt gccaccgcat
    35161 gaagaccgat tttcgccgga atggtgccgc tcgggatatt ccacttcttc tcacagatat
    35221 cacgatgttt ctcgttagtt accaccatgt ccgcaggcag acggtgagca aaggtgccca
    35281 cttcacgcgc agtaccacac gcagaaggtt gcccggtcag ggagaacgga ccgcaacccg
    35341 gctgggaaat tttgccggtc agcaggtgca ggttgtagac caggttgtta gcccacacac
    35401 cacgggtatg ctggttgaag cccatcgtcc agtaggagat gactttcttg ttcggatcgg
    35461 catacagctg cgccagttgt tctaactggt ctttcggcac gccggtcatt tcagcggttt
    35521 tttccagcgt atactcagca acgaaggctt tgtaatcttc aaagctcatc ggttcggagg
    35581 cgtcagaacc cggattcttc gctgcttttt ccagcggatg ggtcggacgt aaaccgtagc
    35641 cgatgtccgt cgcccctttg cgcaggttaa cgtgcttgct gaagaagtcc tgatttatcg
    35701 cattgttttg aatgatatag ttggcgatgt agttcaggat caccaggtca gattgcggcg
    35761 taaagatgat gccgttatcc gccagctcga agctacgatg ctggtaggta gaaagcaccg
    35821 ccacggtgac gttctggtta gagagacgac tgttagtgat gcgtgaccag aggatcgggt
    35881 gcatctccgc catgttagcg ccccacagca caaacgcgtc agcctgctcg atgtcgtcat
    35941 agcagcccat cggctcatcc ataccaaagg tacgcataaa gccaactact gccgacgcca
    36001 tacagtgacg tgcgttcggg tcgatgttgt tcgaacggaa gcccgctttg aacagcttgg
    36061 acgcggcata accttcccag atagtccact gaccagaacc gaacataccg atcgattccg
    36121 gccctttttc tttcagggcg gttttgaatt tctcttccat cacatcgaag gcctgatccc
    36181 aggtgattgg ggtaaattcg ccttctttgt catatttacc gtttttcata cgcagcagcg
    36241 gctgcgtcaa acggtcttta ccgtacatga tcttgggcag gaaatagccc ttaatgcagt
    36301 tcaggccacg gttaaccggt gcgtccgggt cgccctgaca ggccaccaca cgcccctgct
    36361 gcgttccgac cagaacgccg caaccagtac cgcagaaacg gcacggcgct ttatcccatt
    36421 taatggcttc ctgctgacca acaacggcgc gggcaacgcc cggcacgctg agaccggcag
    36481 ccgccgcagc ggccgcaacg gcgttagctt tcataaagct acgacgactg agtttcatgg
    36541 tgtttcctca ccttgctctt cctgctggtg ataaaccagc gacaccgcca gcacgccctc
    36601 tacgttgcgt actgactcaa tggtttggat cagcgtttcg ctgtcttctg cttccaccac
    36661 cacaatcaac tgaccgctcg gcgcgtcgct gacagcaact tcacagccgg gaaaggcgtt
    36721 cagttgggtg ctgatgtctg aaattcgttc gcttttggcc tgcacgacca ggctgcaaac
    36781 ttgccagtta gtgtgcatgg agatactccg cagttatggc tgataccgga cagctggctg
    36841 cacatgctcc acatccgttg caaagttggc tattaagttg cgcctggtag atcccggaga
    36901 gcgtcgggcg aaagataatt gccattggct cacagctatc ctgacagcgg cggcattcaa
    36961 ctgactgata cgccaggcac gcgtccccga tggtgaactg caaatcccag gccctggtgt
    37021 ggcgcggaga aaatagcgat tcggggcaag cctgcgcgca ggcgtaacag aagctgcact
    37081 cgttattttt gaaattaacg ctcggatagc cacccgtgcc gcgttgcaga atgttgtttt
    37141 cacaagcatt aatacaggcg tcgcagcggg tgcaatgggt cagaaaatga gattcatcac
    37201 cggaccaggg cggacggata ccgttactgg ctttgcgcca gcaaccagtg agtatgcccc
    37261 gacgggatgc atcaatcttc acattgacct tccatcatta acgcgctctg aaaattgaga
    37321 gcgaccaaat agaccgcata attaataagc catttttata gccgctaaga tattaaagga
    37381 tgtgtcaaag atgcataccc cgatcggggt aaaacctgta gcaggatcaa aagagtggcg
    37441 ggaagcgtgg caaaaacggg cttttgctca catttcaaat ggttataaac atatttatat
    37501 agcgattaat tcaccagaga tatttctgct ggtttgctcc ctcatcagaa tttaacacta
    37561 aaagagcagg taaaattgtc tgaatgttct ttaagttatt cataaagcaa attaataaat
    37621 ctgatgaata tgttaacctt cagcgacatc atcggtgaaa acctataaat gaagaaggaa
    37681 agcaaaaaaa tgaagaccat tctacctgaa gtattgtttg ccgctttcgc taccacttcc
    37741 gcctgggcgg cagaaagcgt ccagccactg gaaaaaatcg cgccttatcc acaagctgaa
    37801 acaggcatga agcgtcaggt gattcagtta accccgcaag aagatgaatc taccctgaaa
    37861 gtagaactgt taatcggtca gacgctggaa gtcgattgca atttgcatcg tctcggcggg
    37921 aagctggaaa gcaaaacgct ggaaggctgg ggctatgatt attatgtctt tgataaagtt
    37981 agctcgccgg tttccacgat gatggcctgc ccggatggca agaaagagaa gaaatttgtc
    38041 accgcgtatc tgggcgatgc tggaatgctg cgttacaaca gcaagctgcc gatcgtggtg
    38101 tatacgccag acaatgtaga tgtgaaatac cgcgtctgga aggcggaaga gaaaattgac
    38161 aacgcggaag ttcgctaaac tgttgtgaag tacggcaccc cgttggttgg ataaggcggt
    38221 tacgccgcat ccaacatcta acgcccgagc cggttgcctg atgcgacgct ggcgcgtctt
    38281 atcagtatac gccacatccg gcataccatg ccggatgtgg cacatcatta caacgcaata
    38341 tccgccactt ctttttgaac gggttgcggt ttcggcgtac tatctgctgc ttgcggtttg
    38401 tcgtatttca gccccagcac ttcgctggtg tactgcaact cgcgttctgt tgccgctaca
    38461 tcaccgttca gcttgcgtcc ataagacgga acgatcgctt tcaacgtagc ctgccattgc
    38521 gggctggaaa cgcgatcgcc aaatactttt tccagtagat tcaacataat cggcgcggcg
    38581 gttgacgccc ctggcgatgc ccccagcagc gccgcaatgg tcccttgctg gtcactgacc
    38641 acttcggtac ccagacgcag tacgccacct ttctcggcat cacgcttgat aatctgcaca
    38701 cgctgccccg cttgccacaa acgccagtcc tcttttttcg cttgcggata gtactctttc
    38761 aacgcttcaa aacgatcctc ttcactcaac atcacctgac tcaccagata tttcaccaga
    38821 tcgaaattat ccagcccgac gtgcatcatc ggcatcacgt tagaggtggt ggtggaactc
    38881 atcagatccc acaatgaacc gtttttgagg aatttggttg agaaggtggc aaatggccca
    38941 aacagcacta cgcgtttacc gtccagaaca cgggtatcga tatgcggaac cgacatcggt
    39001 ggtgcgccaa cggatgcttt accgtaaact ttcgccagat ggcgattaac cacgtctgga
    39061 ttttccgaaa caaggaactg tccgcccacc gggaaaccgg cgtagtcttt cgcttccgga
    39121 atccccgatt cctgtaacag cttcagcgcc gcaccgcccg cgccgataaa gacaaatttg
    39181 gcgcggatgt tctgtgcagt gccgtttttc agatcggcaa cggtaacggt ccaggtgtta
    39241 tcgtcattac gctttagggc gcggacttcg ctgctgagtt gcagcgagaa gttagatttc
    39301 ttctgtaagg aagcaattaa ctggcgggtg atctcaccat agttcacatc agtgccaatt
    39361 tctgttgcgg atcgcgccct tccatcacta acggtgccca ctctttgatc tgcgcgtgat
    39421 cttcagagta acgcataccg cgaaacagcg agctttgttg caacgcggcg taacgggcgc
    39481 gcaggaaatt aacgttatcc tcgccccaga caaagctcat atgcggaacg gtattgataa
    39541 atgaacgcgg agtacgcagc acgccgcgct cgacctggtg cgcccagaac tggcgggaaa
    39601 tctgaaatgc ttcgttaatg gcgactgctt tttcaatact gatgctgcca tcggcgtttt
    39661 gcggggtgta gttcagttcc atcagtgcag aatgcccggt tccggcgttg ttctagccgt
    39721 tcgaactctc ctgcgcgccc ccctccaggc gctccaccat ggtcatcgac cattcaggct
    39781 ccagctcgcg tcaataggtc cccaacgtgg cgctcataat gccgccgcca atcaacagta
    39841 catcagtttc ctgctcctcg gacgctttcg cttttgccgc catcgaaacg gcattaagcc
    39901 ccacggccat cgagaagagc atggcagtca cttttttcat cttgttaatg ccttactttt
    39961 agtcgcttta ttgcaggtga gatttgcgcg gcatcaacgg taacacttaa gtaacaacat
    40021 ttaaataatg tttataaatt atattctaat tttagaaaca ttataatttc gttgattaat
    40081 tatagggtta ttagaaatga agggattttt agcatattga cctgacggca gcagtccgcc
    40141 aggtcagcgt cgtaatatta tgccgtccgg gcaacggcat cacgcgaagc ggcatcgcgc
    40201 tcttcgcccg tcagctcgct aagttgccca ttgcgcattt ccagcaggcg gtcggcgtgg
    40261 ataaagtaat gatcatcatg actgatagcg aaaatagttt tacccatctc ctgcatcagc
    40321 ggcagcaaca cctgataaaa ctcacgacgg aagtgtggat cctgatccac cgcccattca
    40381 tccagcagga taatatcgcg ttcttctgcc agcgccagca acagcgccac gcgttttttc
    40441 tgcccttttg ataacttcag gttaacaata cgcccgttgc ttaactcaag cttatgagcc
    40501 attttcagct gcgccagcca cttctcaacc agttgcgggt tagcgggttg cccctccggc
    40561 cccagcagtt gatcaaacag ccagacatcg gtaaacactg ccgaaaacag tttgcgataa
    40621 tcttccggtt gttcgccgct gacaggtttg ccatccagca agatttcgcc gctttgtggc
    40681 tgatacaagc ccgtcaacaa catcgccagc gtcgattttc cgctaccgtt accgccaatc
    40741 agaaacagca gctcgccacg tttgatggtg agattaatcg gaccaacgga aaacgcgtta
    40801 tcctgataag caaacgtcac gttacgcagc tccagcgttt gccagttggg aaacgcctgc
    40861 gggcgcggaa actcagcttt gaaaggcgcg agcgcgaatt tgttcagctt gttaaacgcc
    40921 acctgcgccg tcagcagcgt cggcaatgcg ccaaccgccg aaagcagcgg cgtacgcagg
    40981 aataacagcg tcaacgaata ggtcgcagca acgttggtat cagcccaacc gagactgttc
    41041 gccatccaga acaccaggcc gatagcgccc agcatcatga tgtttgacca gttcacggca
    41101 ctaagatgga aggtgtctgc gcgaataata tgatggcgat actcttgcgc atcggggatg
    41161 tagaggttgt taaacacata ctcggcgcgt tcccggttca gagtcagctc tttgcgccct
    41221 tcaagtacag tttgaaaatc cgtgtacagc ttgtcttcgg tttcacgtag ggtcgccata
    41281 tgtttgtaca cccgcgccac cagcacaaaa ccgccccaga tggtgatcgc catccagata
    41341 gccgttacca gcaacatttt gcccgacagc atccacagat acgccgatga accgatagtg
    41401 agaatgatcc cctgccccag ttccggcaga cgcacaaaag caatggtgat attgcgcaca
    41461 tcgctggtta atcccgccag caacgaggcg ctaccgagtt gttcaatgcg ctcgacgtgg
    41521 gtatccagaa tccgcttgat aaattcacta cgcagtcggt aaacgaagtg atgccccaaa
    41581 gtggtgagcg ccagttgcga tccgagagtg actgccatca acagcagcaa taatcccaga
    41641 aactccggta acaccagcag actggtatcc gccgtttcga taaggcgctg attgataaaa
    41701 gcaattaagc caatgcctaa tgccgcactg gcgaggctta gcgccatcac actgataaat
    41761 ggccagcgat actgccgcca gacaagtaca agaagttcca tgcagaaaac ccggacaatg
    41821 aattacagcc cgcagtttaa acatcttcgc gcgcacagca ataataattc ttatttttat
    41881 tcttttttac ctgcctgacg gaatgtcagg ttgtagcggc agtcggtggt gagtggatga
    41941 aaccccgctt tcaacggttg aataccgtga taaaacagcc gcgattcacc gccccatacc
    42001 accacatcgc catgttccaa caacaaacgt ttgagcggat cattacgttt caggccacca
    42061 aattgaaaaa tcgcgggtaa gcccagagaa acagaaacaa ttggcgcgcg cagatccggt
    42121 tcgtctttat cctgatgcag cgacagtttc gcgccaggag cgtagcggtt gataagacaa
    42181 gcatctggct ggaaatctgg atagcccgcc gccgtagccg cacgttgaca taaatcatga
    42241 aagctttgtg gcatggcggg ccacggttta tttgtttgcg gatcaatggg cgaatagaga
    42301 taaccttgcc gatgggtcgt ccagcccaga tgcccacagt tggtcatcgc caccgacatg
    42361 gtatatccgc ctggagcgac catctggcga aacggcgact ggctggcaac gtcattaata
    42421 tcgcggatta gttgctccgc agcattaaaa gcaaaacgcc gtaaaattac cgcaccagcc
    42481 gccagtggct cttgccacgg ttcagcatcg gcaaacagat ccaacattac ctctcctcat
    42541 tttcagcttc gcggcgcagc agttgcgctt tacgcgacac gccccagcgg taaccggaaa
    42601 gtgtgccatc accacggacc actcgatgac agggtattac gatagccagc ttgttggcgg
    42661 cacaggcgct ggcaacggcc cgtaccgctt tcggtttgcc gatggcgtta gccagttgct
    42721 gataactgac ggtttcacca caaggtatcg tgcgcagtgc ctgccagact tgttgctgaa
    42781 aagcagtgcc gcgaatatcc agcggtaacg tcagcggcgt atcgcgttga ttgaggctgg
    42841 cgatcacttc acgcacatgt tgctgaaacg tcagatcggc aggcgcgttg tcggcagcgg
    42901 gaaacatctg ctgcaactcg ctgattaatg tcgcgtcatc atcgcccagc aatatcgcgc
    42961 aaatcccccg ctcgctttct gccaccaggc aacgacccag ctcacaatca gccagcgcgt
    43021 aacgcaccgc cagattttcg ccaccgtgac ggaattgttt agccgtcatg cccagcgttt
    43081 catcggcttt gcggtagtaa ctgctgctgt cggggaatcc ggcgttaaga atagacgtcg
    43141 tcacgctctc ccctttcgcc agcgattcgc gcaaacggcg agcgcgccag gcctgttgcc
    43201 aggctttagg cgtcattccg gtagtagctt taaacaaccg atgtagatga aatggactca
    43261 tcgccacctg gtcggctaag gcttccagcg ttacaggcgt ttcctgttcc agcagtcgac
    43321 acgcgtgggt gattttatcc aaccgatgtt gccggggatt ggctttgtct ggctgacaac
    43381 gtttgcaggg gcgaaatccg gcggcgagtg cctcgctggc atttgcgtag aaggagacat
    43441 tttcccgcaa ggcatgtctg gcgcggcaag aaggacggca aaagataccc gtggtgcgca
    43501 cggcgaaaac gaattcgccg tcggcattcg ggtcgcgggc taagacagat tgccagcgtt
    43561 gatcgtcagt taagcatgtg gcgtttttca taatcagctc cctggttaag gatagccttt
    43621 agtctgcccg gtcaccatca cgcaaaaacc aacaatcttg cgctttaatt tttttcgctg
    43681 acaaggaagc ttttaaactg tggtgacatc caggttttaa agctatcgcc ttctttggtg
    43741 atcatataaa ccgccagccc ttcccggcga acaacctctt tggctttctc cggcccgagt
    43801 accatcaagc cagtatccca ggcatcggct tccagcgccg tcggagcaat caccgtcacg
    43861 gataccagat tgtgttcgat gggacgcccg gtttgcggat cgataacatg ggaaagacgt
    43921 ttgccgtcca gttcgtaata gttacggtag ctgccagagg tgctaatccc atgaccgttg
    43981 atatccacca cagcctgaac cgcgttttct ttatcggttg gtttttgaat cgctacccgc
    44041 cacggctggc cttcaccgtt cataccacgg ctgttcagcg cgccgcccac cgacaccaga
    44101 tagcgggaaa tcccttcctg ctccatcaag cgtgccaggt gatccgccgc ataaccttca
    44161 ccgacggtgg agagatcgac atataaatcc ggcaggtctt tttgcagata ttgctgatgc
    44221 gactgattga tgaccgtcag gtgctgtaag ccggttttgg ctttcatcgc atcgatctgt
    44281 tcctggctcg gaatttgaac cggctgttgt tccgggccaa agccccacag attcaccagc
    44341 ggcccgacgg taatatccat cgcaccatcg gtccttgccc caatgcgcag cgaggtggtg
    44401 acgatatcgg ccatcgcttc acttaccggc cacggcgaca aactttgcga gtcgttaaag
    44461 cgcatcagcg cggaatcttt tttataggtc gaaagcagct gatcgtcggc gtccagctgg
    44521 gtctgaatct tttctttaag ttcggcactg cgtttggcat caatgcccgg gatgctggca
    44581 cgccagaagg tacccatggt tttgccttca agaacggtaa cttcagtagc gtgggttttg
    44641 gcgggttgtg gtttttgatc gcaaccaaca aagaagagcg cggcaaccag cagtgccacg
    44701 cgggtaaagc ttatttccat acgtgattat cctcatgcga acggtcgcaa gagtacacca
    44761 aaaaactgtg tttgtacgct gaaaacaatg aaaaaagggc ccgcaggccc tttgttcgat
    44821 atccatcgag attagaactg gtaaaccaga cccagagcta cgatgttatc agtgttgatg
    44881 ccagcgtcac gagtgaactg gttgtcgtcc agcaggttga ttttgtagtc aacgtaggtg
    44941 gacatgtttt tgttgaagta gtaggtcgca ccaacatcaa catatttcag gatatcttcg
    45001 tcgtcgtagt tacgaccatt gatgacaccc aggtttttac ctttagactg caggtaagcc
    45061 agggacggac gcagaccgaa gtcgaactgg tactgagcaa cagcttcgaa gttctgtgct
    45121 ttgttcgccc aacccaagtt gcctacgcga gttgcgttgt aggtctgggt gtactgagca
    45181 gctaggtaga tgttgttcgc gtcgtatttc agaccaccgg tgtaggtttc agcgcggtcg
    45241 ccattaccga tgtaacgctc atcataacgg ttcagaccga agttctggtc atcagtacgt
    45301 ttggagctgg aaactgcagc accgataccg aagccttcgt aatcataagt aatagaaccg
    45361 ccaacgccgt caccgttctg acgcagagct tcacgaccgt tgttggtcat gccttcacct
    45421 tccgggctac cgtttttgcc ctgatactga acagcgaagt tcagaccgtc aaccagacca
    45481 aagaagtcgg tgctacggta ggttgcgaag ccgttaccac gctgctgcat gaagttgtca
    45541 gaaccgtaag tgtcaccacc gaattctggc agtacgtcgg tccaggaagt tacgtcgtaa
    45601 actacgccgt agttacgacc gtagtcgaaa gaacctacat cctggaattt cagacctgcg
    45661 aatgccacac gggtccagga gttgttttcg ttttcagcgc tgttgccctg gatctgatat
    45721 tcccactggc cgtaaccggt cagctggtca gtaacctgag tttcaccttt gaagccaaga
    45781 cgcatgtagg tctggtcgcc atctacagac ttgtcgtcag agaaatagtg caggccgtct
    45841 actttaccgt acagatctaa tttgttgccg tctttgttgt aaacttcagc agcgtttgct
    45901 gcgcctgcta ccagcagagc tgggaccagg agggacagta ctttaacttt catgttatta
    45961 accctctgtt atatgccttt atttgctttt ttatgccact gcatactgat taaccctcat
    46021 taatcagtcg gcaagtccat tctccccaaa aatgcagaat aatccaacac gaatatgata
    46081 ctaaaacttt taagatgttt catttatcgc tatagatgtt tcaaaatgta aatacaaggg
    46141 aactttttaa gattattgcg gaatggcgaa ataagcacct aacatcaagc aataataatt
    46201 caaggttaaa atcaataact tattcttaag tatttgacag cactgaatgt caaaacaaaa
    46261 ccttcactcg caactagaat aactcccgct atcatcatta actttattta ttaccgtcat
    46321 tcatttctga atgtctgttt acccctattt caaccggatg cctcgcattc ggttttttta
    46381 cccttcttta cacacttttc attattctgt gctaccacag aaaaactata acgcttgtta
    46441 actatttcac aaataattaa catcagcata atttccagca atctttgttt atttgcaatt
    46501 aatttcgttg ggctttttgt aggttatttg tacagcaaaa tggcgcttgt acatctattt
    46561 ccccccatac aggatgataa atatcacggg agaatagaga atcatcaatc aggtaagagt
    46621 ctggaatttc acactgtacc ctttatactg ccctatcact tcgcgaagtt ttaacaggtc
    46681 ataaacacga atgcgtcaga aagagacaac ggccacgacc cgcttttcac tcctaccggg
    46741 gagcattacc cgcttctttt tactgttgat cattgtgtta ctggtgacga tgggggtaat
    46801 ggtacaaagt gccgttaacg cttggctgaa agataaaagt taccagattg tcgacattac
    46861 ccacgcaatc caaaagcgcg tcgatacctg gcgttacgtg accaggcaga tctacgacaa
    46921 cattgccgcg acgacctccc cctcctccgg cgaaggttta caagagacgc gcctgaaaca
    46981 ggatgtctac tatctggaga aaccacgccg caaaacggaa gcgttaatct ttggctctca
    47041 cgacaactca acgcttgaga tgactcagcg gatgtccact tatctggata cattgtgggg
    47101 tgcagaaaat gtaccgtggt cgatgtatta cctgaatggt caggataaca gtctggtgct
    47161 gatctcaacc ctacccctta aagatctcac ctccggattt aaagaatcga ccgtcagtga
    47221 cattgttgat tcacgtcgtg cagaaatgtt acaacaggcc aacgccctcg atgaacgcga
    47281 aagtttttct aacatgcgcc gcctagcctg gcagaacggt cattacttta ccttgcgtac
    47341 cacgttcaac cagccgggac atctggcaac ggtcgtggct tttgatctgc cgattaatga
    47401 tttgatccca ccgggtatgc cgctggacag tttccgcctt gagccagacg cgacggcaac
    47461 gggaaacaat gataatgaga aagaagggac ggatagcgtc agtatccact ttaacagtac
    47521 gaagattgaa atctcctcgg cactcaactc taccgatatg cgcctggtct ggcaggttcc
    47581 ttatggcacc ttattgctgg atacgttgca aaacattctg ctgccactgc tgctgaacat
    47641 cggtttgctg gcgctggcgt tatttggcta taccacattc cgccatttct ccagccgcag
    47701 tacagaaagc gtccccagca cggcggtcaa taacgaattg cgcattttac gggcaatcaa
    47761 tgaagagata gtctcactgt tgccgctcgg cctgctggtt cacgatcagg aatcgaaccg
    47821 cactgtcata agtaacaaaa ttgccgatca tttgctgccg catctgaatc tgcaaaacat
    47881 caccaccatg gcggaacagc atcaggggat tattcaggcg acgatcaata acgagctgta
    47941 tgagatccgc atgttccgca gccaggtcgc gccgcgcaca caaattttca ttattcgcga
    48001 tcaggatcgc gaagtgctgg taaacaagaa actcaagcag gcgcagcgtc tgtatgagaa
    48061 aaaccagcag gggcggatga cctttatgaa aaacattggc gatgcgctga aagaacccgc
    48121 acagtccctg gcggagagtg cggctaaact caacgccccg gaaagcaaac aactggcgaa
    48181 tcaggcagat gtgctggtgc ggctggtcga agaaatacag ttagcgaaca tgcttgcgga
    48241 tgatagctgg aaaagtgaga cggtgctgtt ctccgtgcag gatttaattg atgaagttgt
    48301 gccttcagtg ttgcctgcca tcaagcgtaa aggtctgcaa ctgctgatta acaatcatct
    48361 gaaagcacac gatatgcgcc gcggcgatcg cgatgcctta cgacgtattt tgctgctact
    48421 gatgcaatat gccgtgacct caacgcaatt gggaaaaatc acccttgagg ttgatcagga
    48481 tgagtcctct gaagaccgcc tgacattccg cattctggac acgggagaag gcgtaagtat
    48541 tcatgaaatg gataatttgc acttcccgtt tatcaaccag acccaaaacg atcgttatgg
    48601 aaaggcggac ccgctggcat tctggctgag cgatcaactg gcacgtaaac tgggcggtca
    48661 tttaaacatc aaaacgcggg atgggcttgg tacacgctac tctgtgcata tcaaaatgct
    48721 cgccgctgac ccggaagttg aagaggaaga agagcgttta ctggatgatg tctgcgtaat
    48781 ggtggatgtt acttcggcag aaattcggaa tattgtcact cgcctgttag aaaattgggg
    48841 tgcaacctgt atcacacccg atgaaagatt aattagtcaa gattatgata tctttttaac
    48901 ggataatccg tctaatctta ctgcctctgg cttgctttta agcgatgatg agtctggcgt
    48961 acgagaaatt gggcctggtc aattgtgcgt caacttcaat atgagcaacg ctatgcagga
    49021 agcggtctta caattaattg aagtgcaact ggcacaggaa gaggtgacag aatcgcctct
    49081 gggcggagat gaaaatgcgc aactccatgc cagcggctat tatgcgctct ttgtagacac
    49141 agtaccggat gatgttaaga ggctgtatac tgaagcagca accagtgact ttgctgcgtt
    49201 agcccaaacg gctcatcgtc ttaaaggcgt atttgccatg ctaaatctgg tacccggcaa
    49261 gcagttatgt gaaacgctgg aacatctgat tcgtgagaag gatgttccag gaatagaaaa
    49321 atacatcagc gacattgaca gttatgtcaa gagcttgctg tagcaaggta gcctattaca
    49381 tgaacaatat gaacgtaatt attgccgatg accatccgat agtcttgttc ggtattcgca
    49441 aatcacttga gcaaattgag tgggtgaatg ttgtcggcga atttgaagac tctacagcac
    49501 tgatcaacaa cctgccgaaa ctggatgcgc atgtgttgat taccgatctc tccatgcctg
    49561 gcgataagta cggcgatggc attaccttaa tcaagtacat caagcgccat ttcccaagcc
    49621 tgtcgatcat tgttctgact atgaacaaca acccggcgat tcttagtgcg gtattggatc
    49681 tggatatcga agggatcgtg ctgaaacaag gtgcaccgac cgatctgccg aaagctctcg
    49741 ccgcgctgca gaaagggaag aaatttaccc cggaaagcgt ttctcgcctg ttggaaaaaa
    49801 tcagtgctgg tggttacggt gacaagcgtc tctcgccaaa agagagtgaa gttctgcgcc
    49861 tgtttgcgga aggcttccta gtgaccgaga tcgctaaaaa gctgaaccgc agtattaaaa
    49921 ccatcagtag ccagaagaaa tctgcgatga tgaagctggg cgtcgagaac gatatcgccc
    49981 tgctgaatta tctctcttct gtgaccttaa gtccggcaga taaagactaa tcatctgtag
    50041 gccagataag acgcgttagt gtcttatctg gcatttgcac cgattgccgg atgcggcgta
    50101 aacgccttat ccggcctacg attcccatta tttcaacaga ttacattaaa gtaggccaga
    50161 taagacgcgt cagtgtctta tctggcattt gcactgaatg ccggatgcgg cgtaaacgcc
    50221 ttatccgtcc tacgaatccc gcgatttcct gaccctctcg gcatataccg tcagcgtctg
    50281 ttttatcaca tccagcgtta ccggcttcga gaggcagctg tccataccgg actccagaca
    50341 ccgctgcttc tcttcagcca acgcattagc agttactccg attaccggca acgtcagtcc
    50401 caactgacga atgcgttgcg tcaagcggta accatccata tttggcatgt tgacgtcgct
    50461 aagcacgata tcaatatgat tcttactaag tacattaagc gcatcgacgc catcattcgc
    50521 ggttttacat tgatagccca acgatcccaa ctgatctgcc agcagacgtc ggttaatcgg
    50581 atgatcatcc acgaccagaa tcatcatatc gtcgttatcg ctgaccgctt tgtccgtcga
    50641 cggcagagcg ttagcaggat cgtcgctctc catctcgatc aaataaatac gcgccaacaa
    50701 tgccggtagc tcatgcggag cagccacact gtgtacccac tcccctggcg ctttctccag
    50761 cggaatacca atatggcgac gacagaaggt cactaccgct ctgccctgcc attttttact
    50821 cactacctcg tcagtgatta acacatcttc gggagtcggt tcctgccctt cgtatgttgt
    50881 aacgacgatg ccgctgcgct gcaaactggt ttccaggaac tgacagagcg acgcattgcg
    50941 gaccgccagc cagcagcgtt taccactcaa cccttccacg ccttttttct gcgggtactg
    51001 agcgccgtac aacggaatac gcacggtaaa ctggctgccc attcccggtt ctgaatctac
    51061 cgagatatcg ccgtccatca tgctgatcag tttttcacaa atcgccagac ccagaccggt
    51121 cccctggaag tttcgctgta cgcccgttcc aacctggaag aagggatcaa acaagcgcac
    51181 cacttctttc gccggaatcc ccacgccggt atcgcgaaca cggatagaga gataatcgcc
    51241 atcagcgcga acatgcaaaa ctatacagcc ggtatcggtg aattttatgg cgttactcaa
    51301 caggttggag atgacctgct gtaaacgcat cgggtcgcca tttaaggcca ctggcacatc
    51361 cggttcaata aagcagtaca agcctaactg cttgcgtacc accagcggta aatagttggc
    51421 ggtgatgtgg ttcatcactt cacgcggtga aaactcacgc ggttcgatct tcaactgttc
    51481 cgattcaatc ttcgagaaat cgagaatatc gctgataatt ttcaacaaca ggctggaaga
    51541 gttgttcatt accgtcacca gacgatcgac gcctttcggt aactctttgg tttgcaacag
    51601 atccaggtta ccgataatgc catacagcgg cgttcgcagc tcatgactga cggtggcaag
    51661 gaacatcgat tttgactggc tcgcctgttc cgctgcttgt gccatctcct gcaacgactc
    51721 ttccatcttc acgcgcgaag aaacatccac cagcacacaa atggccacgt tttcattacg
    51781 atagcgcgaa tggacgaagc tgatttgcag attggtattg ttgctggtca ggacatcaac
    51841 aaaattgacc tgctgcccac agataatttg cgtcagtcgt tggcggtcct catgcgtaag
    51901 catattgaga taggtatgcg ccagttcgtt acttaaaata ttgacgccat cagcggtacg
    51961 caaaatgcag atacccactg gcgcggaggc gacaatcttg cgattgaact gctcatgttc
    52021 ttccagtcgc agggcgtcgc tttccgccgg aatgaaaata cgtcgctcgt acatccgcgc
    52081 gagagtaaac aatgcagctc cggcaagcac attcagcaaa attgcgttaa ggatcaacat
    52141 gcgaatgcgt tccagcacct tatcaaccgg caccgaatac acgatgctta gcgatgaggg
    52201 tggcagattt ttcttcagca ccagctcccg gaacccttcc gtatagccaa accaggagcg
    52261 ttcctgcatc cagcgaggat cgcccttaat tttactttct ggtccggtaa gcgaaatcag
    52321 ggtatgacca ttttcatcaa gaatggtaac ccccatcggc aacgtacccg gtaagaaaaa
    52381 gttctccatc cggatggtct gctcgacacc caaaagcgcc tgcaaccggt tcgccagata
    52441 aactggcgtc aacgcgtaaa aatacccgac gcaagggcgc ggaccttcgc tgatccagta
    52501 gaggttactg ccgctatcat cttgtggtgc atttcgatat ttattgatgc gttcatgcaa
    52561 cgctttcaac gcggtatcgc gttccactgg catatcacgc agaccgaaat tggccatgca
    52621 gaggttatca ctgccgatta aaaatacccg gttgagatcg taagccgcag aaaaattatc
    52681 gcgccagtag cgcataaacc acgccaatga ctccagagaa cctcgccagg tgttactcat
    52741 tgcggaacaa tcggagtcgg caaacagcgg ttcaaacgca ggcacatccg cctgcgtttc
    52801 tcgtccacgc ggggaaagca caccgttttc tgccgataag cgattttcgg cgatgtactt
    52861 aagctctttc atcacatcag aggtgcgttg aataaagcgc tgagcctgat cggaactcag
    52921 attaaattcc tgacgaattt ccgattctcg ctgatgtaac gcattaacga tgtaaaaaac
    52981 ggatgaaaaa gcaatcaaca gccagagcac taacgccaat gctctgaaca tgtagcgcga
    53041 ggctttcagg gttgtacgaa aagaagcaag gtatttcaaa ggggagaagc tccgcctcag
    53101 gtgaccgatg gagtgtggtt aaggtagcgg taaaagcgtg ttaccgcaat gttctgtcac
    53161 cattgcaata atgcgtatcg cttagaaacc attgccttaa ccaggaaagc cggagtagat
    53221 ggacagttct cagtacgaaa aataagaaat gaccaatctg ccgacgcttg ttaatggtta
    53281 tatcctggcg tcggcagatc gcccagcagt cttatctgaa aaaagagaga gccaggttgt
    53341 gtgaagggat ggatcaccag tcaaaacttt tcaccagcgt cagctcgcca gcattacgca
    53401 tcggtacaat aaatgtttcc tgtttctcat tgaccgatcc ttcatcggtg atcagcgtca
    53461 gttgggcggt cgtcaattcc gtttcgctgc gcccaccata gtagttgata tacacctgat
    53521 agcgcccgtg aactggtgct ggcatggcga aaatctcggg tccgtacccc gtcgtgacat
    53581 ccatatccag tgcaccactg tttttcagca cggtgttacc gtaccaggcg tgttcgccat
    53641 cgggcgtgac aacatggaga tcgagatcgg tattgtccgt atcccacgag agaaccagcc
    53701 gtaaacgtgc acgaatagtt cctgtacccg gcgttgagta aaactgcatt ttttgccggc
    53761 tttgcccatc cgggctgatg acctgcacgc tgttgctgcc ttcagtgaaa atataagggc
    53821 gtgcaaatga accatcggat tcaattctct gcggcatact ggcaccgttg accaccagcc
    53881 ggccctgctg aactttaccc gccgcagcat aattctttat tttgccgcgg atctgcgctg
    53941 agatattttg atcatctgcc atgttgacgg acgacgccgg gtaattgatc gattgtgaaa
    54001 aactggcatc ttcgccctct gccggatgcc agccagagag cggtgcgtca atctcgactt
    54061 cctgcacacc ttcactgtga gcgacaggcg aaagtgccac cagtaacaac ggaagaaaaa
    54121 tctttcgcat agcattagtc cattaataat tgtcgggtca gcgtttcgat atagttctca
    54181 tcaagcccgg tgggataacc atcaaaggcc aagtgcagat actcatgggt taaatccagc
    54241 cgatcctgaa gcgtaaagaa gttacgaata aacagccgtt tctgctgccg atcggtatag
    54301 gggaaacctg agactaaacg acagaccgca aacacgtctg gttcgttgta ccccgtctcg
    54361 ccttgtaata tacgccgcca ctgcggcatt tttttcgcca gccaggcttt cgctttgggt
    54421 aataactggc aggttgagcg tggagctccc cagcggctaa ggctgttgtc gggataagca
    54481 aacgcgagta tctggtcgta acgctccccc tgccccgcct gggccgtggc ttgccgccag
    54541 gaaagtgtcc cttcggtagc gcgactgcca tgataatgaa ccggatcgcc tgcatagatg
    54601 agctcctgcg tccaggcggt catcgttcgc gcccccgtgg tcgctggcga agcagaaaca
    54661 cgctgcgtgg cgctgctgtc ggggatggtc aggcaatcac cttcccgatt ggcgttttgc
    54721 tgtaaaaaag tacgtatcgc cacagtcatg gctttcgctg cctcaggtgg cgtacttttc
    54781 gcctcgcgat ccagaactct ggtaacgtac tcttcacgat cgagatgcga ctgcaatttc
    54841 agtttgccct tttcgctcag taaggtcgtc tcaccgtggc tgacaaacgt aatgtggtta
    54901 ccattagtaa acgtcacacg atagcgccca ttaagtacgc tgggttttac tgccgtcgta
    54961 ctcttttccg ctgtgatttt cttcagcgga tagcgggcaa acagctccac ctctacacat
    55021 tgcccactgg caacctgcgt cggcactggc aaaacccgat taagcaccgt cgcataacgg
    55081 gttagtaccg ttttgctggt gccactgcca gtaacccaca gcggtgtacc atcggttaac
    55141 cagccagcaa aaccaccctg acggatttcc tgtttatcat cggcaaacca gctccaggtt
    55201 ttcacccgta cccgactacc gagcatggat gcgacaccga ttttcgcctc gtccagcacc
    55261 acatccagta agacttcctg agctttgttt tgcgcaggta acgtcgctaa tgaatcaagc
    55321 agcgatttaa ccgttaccga ggtttctggc ttcagcgttg taagagacgc taaccacgca
    55381 ggtgcctgac gctgttgcca gtattgcccc cacacatctg cgccaatatg caaccgctgc
    55441 ggcgcaaaat agagcccaca ggaacgtacc agcgcggtat cgcgcgttat gctctcccca
    55501 gcctgacaac aataaacctc ttccggggag tttccgcgac actggtaggg ctgctccggt
    55561 tgatgggtat cctccagcca ggcgtaaaca tacagtttcc acaaactccc taacggcgtg
    55621 gtgagcgagt caggtaatgc tgagactttg gtcacgcctg atgacgacag ctgatacagt
    55681 tgatcgtgct gcgcaccgcg cagcaccagt tgtaacggcg tctcctccgc aagcgttggc
    55741 agagtaacca acgccaatag ccagacgatt ctgcgccagt tcacttattt cacctgcatc
    55801 ccggtccatt cactccctgc cgcgacactt tgctgcgcag gtgcatagga acgcacataa
    55861 cgagcaggag gcagaacgaa ttgccctttt tgcgagaagc gcagcaaatg gcggaaagtg
    55921 accgttcccg tcagttcttt caccggcacc atataggcca gttcgcccat ttcattacgc
    55981 gctttttcca gcaattgccc ctgctgtttc gcggcgttgg gtttattgac tgaaatgccc
    56041 catgttgtgc gctcaacgtc ggctcccggc gggagcggta cttccacctg accatagcgc
    56101 agaactgcat cctgctcgct ggtaagcgta atttcatcga gatacagcgc atcgctgtca
    56161 atctcattgc tggtcaccgg ctgcagagta aagctcatct cttcttcacc ggggataagc
    56221 cgatacaact ggcgttcaac ggtcaccgga atgttacttt gttgagccgt ttttgccggt
    56281 tcacgccagc ggacctgcac atttgcggcg ataattcgtc accaaaagag agaatgtccg
    56341 gcacacccta accaacccaa cgccagtctt cgccccctcc agttaactta tgtttagccc
    56401 atgcgcccgc aggcgcaggc aaaacaactg gaggcattgt cgccatatat ttcgccagcc
    56461 agttcatggc cagcgcgcgc tcaatagtgg attgctcagc ggtcaaacca ttaaaatagc
    56521 ggccgtctgg gtagcgtcac caccgctatt gagcagcaca accgtgtgcg ccagcggctg
    56581 attgctgttt gccgctttat cccacgcata ttgctgaacc tgtggcatca ccgtggactt
    56641 caactgcgct tttttcagca acgtcatggt taagacgttt gccaccgcat ctgccagtgg
    56701 agattccggt gtatcgagga tcaggctgtc attgatatcg cggatgtctt cctcagagaa
    56761 atcttcatct ttagttccgc gccgggcgat agcttcatcc ccccctttca acaacgtttt
    56821 gcacggcaga ttcatctcct gtgcccacgc cagcaccagc gcccgatgca ataacggcat
    56881 gttatccgcc tgctcggcat aactgtcgag catatgctgc cagtattccg gttgttgcgt
    56941 tacgccgagc gcctggctgg cctgccagtc ggcgtaccat gcccatgccg taaggaaggc
    57001 gtcaccattg ccatcttcac cccaccaggt aaagcgcgct ccgggccccg ccagttgcat
    57061 cagccgcaga cggttatcct gaatcatctg acgaatgtcg ttagcggcgg cactttgatg
    57121 atcggcaagc gaacgccagg cgagactgag cgggatcaga cggctaccgg tgttgatcac
    57181 cccaccccac ggttcatcga ctaacgcatc aagattgttg cggaaaatct cctgcggcgt
    57241 ttcactactt tgtagccgga tattactcgc ctgctcgggc aacatcagcg cgttctcgcc
    57301 accgccgagc atgacatttt tctgctgttc aacgggccag ctgttgtcca caaaagacag
    57361 ttttgtgcta atgctgtcct gcacttgccc attttgttgc agttctgcac ttaacaagcc
    57421 agattgctga atattctgcg tcagcgaaat ataattcgcc cctttgtgca gcgtcagcgt
    57481 ctggcgcatc tcagcgcctg caaatttagt caccagcgcc accggttcgt tatcctgctg
    57541 actgaagata aacagtcctg ccgccggttt gtcgcccacg cgatacaccg ttggcatact
    57601 ccacttcatg tagagatttt tttccgaacg cagataagca cgcccctgcc cgaccagccc
    57661 gtcgccgttc atcccacgcg cggtgatacg ccagcgggtt aacgaatcag gcatcaggaa
    57721 cgtgaaatac gctttgcctt gtttatcggt tgtgagtgac ggcatccatg ccgcggtatc
    57781 cacctcttca cgccgtggac gttcaggcat ttttactcgc cgctcgctgc ggttagtcgc
    57841 gccaggcgca accggctcgc tagagagcgc ctggtcatag ctgataaacg acagactgga
    57901 gctggtacgc acattgttac gccccagcgg atagaaaaat ttgccgatat tcggcgcgat
    57961 ttctggttgc agcgcgtaga tcatttcatc gaccacgcct accgttagct gcgcagaaac
    58021 aggtttacct ttcagcgacg aggttaatt
//
LOCUS       DAJTQY010000016        55417 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.016, whole genome shotgun sequence.
ACCESSION   DAJTQY010000016 DAJTQY010000000
VERSION     DAJTQY010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 55417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55417
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.016"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(103..1476)
                     /gene="argH"
                     /locus_tag="OO349_000992"
     CDS             complement(103..1476)
                     /gene="argH"
                     /locus_tag="OO349_000992"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HCR5866159.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLSELQKFSQVIGEDVYPILSLQSCLEKRAAKGGVSPQQVAQAIA
                     FAQARLE"
     gene            complement(1537..2313)
                     /gene="argB"
                     /locus_tag="OO349_000993"
     CDS             complement(1537..2313)
                     /gene="argB"
                     /locus_tag="OO349_000993"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HCR5866160.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRYAEQLPALFNGMPMGTRILA"
     gene            complement(2321..3325)
                     /gene="argC"
                     /locus_tag="OO349_000994"
     CDS             complement(2321..3325)
                     /gene="argC"
                     /locus_tag="OO349_000994"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HCR5866161.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            3479..4630
                     /gene="argE"
                     /locus_tag="OO349_000995"
     CDS             3479..4630
                     /gene="argE"
                     /locus_tag="OO349_000995"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HCR5866162.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASYGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGINAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPQPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            4982..7633
                     /gene="ppc"
                     /locus_tag="OO349_000996"
     CDS             4982..7633
                     /gene="ppc"
                     /locus_tag="OO349_000996"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HCR5866163.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYERNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            7816..9549
                     /locus_tag="OO349_000997"
     CDS             7816..9549
                     /locus_tag="OO349_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HCR5866164.1"
                     /translation="MHSTEVQAKPLSSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWTASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            9698..10549
                     /locus_tag="OO349_000998"
     CDS             9698..10549
                     /locus_tag="OO349_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5866165.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTEHSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(10536..10877)
                     /locus_tag="OO349_000999"
     CDS             complement(10536..10877)
                     /locus_tag="OO349_000999"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCR5866166.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(10879..11757)
                     /locus_tag="OO349_001000"
     CDS             complement(10879..11757)
                     /locus_tag="OO349_001000"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HCR5866167.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            complement(11723..14020)
                     /locus_tag="OO349_001001"
     CDS             complement(11723..14020)
                     /locus_tag="OO349_001001"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCR5866168.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATNTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAQHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDTYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRVFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            complement(14071..14391)
                     /locus_tag="OO349_001002"
     CDS             complement(14071..14391)
                     /locus_tag="OO349_001002"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCR5866169.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(14406..15485)
                     /locus_tag="OO349_001003"
     CDS             complement(14406..15485)
                     /locus_tag="OO349_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HCR5866170.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIIGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            15794..16897
                     /locus_tag="OO349_001004"
     CDS             15794..16897
                     /locus_tag="OO349_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HCR5866171.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLVGDNCQLLISGADEQEGHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALSNLPAAKDVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLHEHLLAGVSAGLSCAEAIVAS
                     ANHFCEEFARSSSRYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQSAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRV"
     gene            16898..18295
                     /gene="ptsP"
                     /locus_tag="OO349_001005"
     CDS             16898..18295
                     /gene="ptsP"
                     /locus_tag="OO349_001005"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR5866172.1"
                     /translation="MTQQARTADGIRIEIAANIAHSVEAQAAFGNGAEGVGLFRTEML
                     YMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGGDKPVDYLNIPAEANPFLGYR
                     AVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSMEEILWVKEKLAEAKQQLRNE
                     HIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSNDLTQYLLAVDRDNAKVTRHY
                     NSLNPAFLRALDYDVQAVHRQGKWIGLCGELGAKGSVLPLLVGLGLDELSMSAPSIPA
                     AKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRMTQQDAPLVTAECITLESDWR
                     SKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFSTGLGFSFAIPHSKSEHIEQST
                     ISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIFSRLARRIMHEEFRNALVNAA
                     SADAIASLLQHELEL"
     gene            18307..18969
                     /gene="fsa"
                     /locus_tag="OO349_001006"
                     /pseudo
     CDS             18307..18969
                     /gene="fsa"
                     /locus_tag="OO349_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
     gene            18980..20081
                     /gene="gldA"
                     /locus_tag="OO349_001007"
                     /pseudo
     CDS             18980..20081
                     /gene="gldA"
                     /locus_tag="OO349_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
     gene            20356..20973
                     /locus_tag="OO349_001008"
     CDS             20356..20973
                     /locus_tag="OO349_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HCR5866173.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYMQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWKLKRPDSNIDHRRVPNLETWFSRHNKTRPISKNAGDYQAGDIVSWRLDNGLA
                     HIGVASDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(21000..21905)
                     /gene="yijE"
                     /locus_tag="OO349_001009"
     CDS             complement(21000..21905)
                     /gene="yijE"
                     /locus_tag="OO349_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HCR5866174.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(21999..24179)
                     /gene="katG"
                     /locus_tag="OO349_001010"
     CDS             complement(21999..24179)
                     /gene="katG"
                     /locus_tag="OO349_001010"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HCR5866175.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(24508..25398)
                     /gene="metF"
                     /locus_tag="OO349_001011"
     CDS             complement(24508..25398)
                     /gene="metF"
                     /locus_tag="OO349_001011"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HCR5866176.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            complement(25747..28179)
                     /gene="metL"
                     /locus_tag="OO349_001012"
     CDS             complement(25747..28179)
                     /gene="metL"
                     /locus_tag="OO349_001012"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HCR5866177.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEFSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPSKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDYPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(28182..29342)
                     /gene="metB"
                     /locus_tag="OO349_001013"
     CDS             complement(28182..29342)
                     /gene="metB"
                     /locus_tag="OO349_001013"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HCR5866178.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLCRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            29619..29936
                     /gene="metJ"
                     /locus_tag="OO349_001014"
     CDS             29619..29936
                     /gene="metJ"
                     /locus_tag="OO349_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HCR5866179.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            30120..30728
                     /locus_tag="OO349_001015"
     CDS             30120..30728
                     /locus_tag="OO349_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HCR5866180.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYVFEAVGPVKYTPLKQWIAHGEKGNYVVRRVEGGLSVEQQQK
                     LTQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(30789..31001)
                     /gene="rpmE"
                     /locus_tag="OO349_001016"
     CDS             complement(30789..31001)
                     /gene="rpmE"
                     /locus_tag="OO349_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HCR5866181.1"
                     /translation="MKKDIHPKYEEITASCSCGNVIKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            31204..33402
                     /gene="priA"
                     /locus_tag="OO349_001017"
     CDS             31204..33402
                     /gene="priA"
                     /locus_tag="OO349_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HCR5866182.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRMRVPFGKQQERIG
                     IVVSVSDASELPLTELKAVVEVLDGEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            33558..34583
                     /gene="cytR"
                     /locus_tag="OO349_001018"
     CDS             33558..34583
                     /gene="cytR"
                     /locus_tag="OO349_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HCR5866183.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            34675..35634
                     /gene="ftsN"
                     /locus_tag="OO349_001019"
     CDS             34675..35634
                     /gene="ftsN"
                     /locus_tag="OO349_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HCR5866184.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            35727..36257
                     /gene="hslV"
                     /locus_tag="OO349_001020"
     CDS             35727..36257
                     /gene="hslV"
                     /locus_tag="OO349_001020"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HCR5866185.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            36267..37598
                     /gene="hslU"
                     /locus_tag="OO349_001021"
     CDS             36267..37598
                     /gene="hslU"
                     /locus_tag="OO349_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HCR5866186.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            37665..38591
                     /gene="menA"
                     /locus_tag="OO349_001022"
     CDS             37665..38591
                     /gene="menA"
                     /locus_tag="OO349_001022"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HCR5866187.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQKMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            38684..39169
                     /gene="rraA"
                     /locus_tag="OO349_001023"
     CDS             38684..39169
                     /gene="rraA"
                     /locus_tag="OO349_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HCR5866188.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(39254..39499)
                     /gene="zapB"
                     /locus_tag="OO349_001024"
     CDS             complement(39254..39499)
                     /gene="zapB"
                     /locus_tag="OO349_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HCR5866189.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            39925..40770
                     /gene="glpF"
                     /locus_tag="OO349_001025"
                     /pseudo
     CDS             39925..40770
                     /gene="glpF"
                     /locus_tag="OO349_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
     gene            40810..42300
                     /gene="glpK"
                     /locus_tag="OO349_001026"
     CDS             40810..42300
                     /gene="glpK"
                     /locus_tag="OO349_001026"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HCR5866190.1"
                     /translation="MVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPGWVEHDP
                     MEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAIVWQCRR
                     TAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGELLFGTV
                     DTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEVRRSSEV
                     YGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNTGEKAVK
                     SENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFATKVQNT
                     NGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDVLEAMQA
                     DSGIRLHALRVDGGAVANNFLMQFQSDVLGTRVERPEVREVTALGAAYLAGLAVGFWQ
                     NLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            42530..43540
                     /gene="glpX"
                     /locus_tag="OO349_001027"
     CDS             42530..43540
                     /gene="glpX"
                     /locus_tag="OO349_001027"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCR5866191.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            43637..44383
                     /gene="fpr"
                     /locus_tag="OO349_001028"
     CDS             43637..44383
                     /gene="fpr"
                     /locus_tag="OO349_001028"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HCR5866192.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(44388..44816)
                     /gene="uspD"
                     /locus_tag="OO349_001029"
     CDS             complement(44388..44816)
                     /gene="uspD"
                     /locus_tag="OO349_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HCR5866193.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(44843..45142)
                     /locus_tag="OO349_001030"
     CDS             complement(44843..45142)
                     /locus_tag="OO349_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HCR5866194.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(45354..45794)
                     /locus_tag="OO349_001031"
                     /pseudo
     CDS             complement(45354..45794)
                     /locus_tag="OO349_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
     gene            45895..46494
                     /locus_tag="OO349_001032"
     CDS             45895..46494
                     /locus_tag="OO349_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HCR5866195.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            46602..47369
                     /gene="tpiA"
                     /locus_tag="OO349_001033"
     CDS             46602..47369
                     /gene="tpiA"
                     /locus_tag="OO349_001033"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HCR5866196.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(47424..48179)
                     /gene="cdh"
                     /locus_tag="OO349_001034"
     CDS             complement(47424..48179)
                     /gene="cdh"
                     /locus_tag="OO349_001034"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HCR5866197.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDKNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHECEILR"
     gene            complement(48286..49275)
                     /gene="sbp"
                     /locus_tag="OO349_001035"
     CDS             complement(48286..49275)
                     /gene="sbp"
                     /locus_tag="OO349_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HCR5866198.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     SHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(49595..50557)
                     /gene="pfkA"
                     /locus_tag="OO349_001036"
     CDS             complement(49595..50557)
                     /gene="pfkA"
                     /locus_tag="OO349_001036"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HCR5866199.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(50738..51640)
                     /gene="fieF"
                     /locus_tag="OO349_001037"
     CDS             complement(50738..51640)
                     /gene="fieF"
                     /locus_tag="OO349_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HCR5866200.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(51788..52288)
                     /gene="cpxP"
                     /locus_tag="OO349_001038"
     CDS             complement(51788..52288)
                     /gene="cpxP"
                     /locus_tag="OO349_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HCR5866201.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            52438..53136
                     /gene="cpxR"
                     /locus_tag="OO349_001039"
     CDS             52438..53136
                     /gene="cpxR"
                     /locus_tag="OO349_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HCR5866202.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            53133..54506
                     /gene="cpxA"
                     /locus_tag="OO349_001040"
     CDS             53133..54506
                     /gene="cpxA"
                     /locus_tag="OO349_001040"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HCR5866203.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(54612..55286)
                     /gene="yiiM"
                     /locus_tag="OO349_001041"
     CDS             complement(54612..55286)
                     /gene="yiiM"
                     /locus_tag="OO349_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HCR5866204.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
BASE COUNT        13501 a        15018 c        14156 g        12742 t
ORIGIN      
        1 aaatttctgc ctgatgcggc gtaaacgcct tatccgccta cgaagtgtcg cactttgtag
       61 gcgtggtaaa ctccccattt tcaggggagc aatataaaca aattactcta accgagcctg
      121 cgcaaaagca atcgcctgcg ccacctgctg cggtgagacg ccgccttttg ccgcacgctt
      181 ttcgaggcac gattgtagcg acagaatcgg atagacatct tcgccaatca cctgactgaa
      241 tttctgcaac tcactgagtg gcagatcttc cagcggtttg ccctgacgaa tggcttccac
      301 caccgcttca cccacaatat ggtgcgcctc gcggaaaggt acgcctttcg ccaccaggta
      361 atccgccagt tcagtggcgt tcgcataccc ctgctgcgcc gcttcctgac aacgcggacg
      421 tttcacctga atgccgtcca gtaccagcgc cgccatatgc aggcagtcca gccaggtatc
      481 gagcgcgtcg aacagacctt ctttgtcttc ctgcatatct ttgttgtaag ccagcggcaa
      541 acctttcagc gtcatcatca tgccggttaa cgcaccctgc acccggccgc atttaccgcg
      601 aatcagctcc agcgcatccg ggtttttctt ctgcggcatt aatgatgaac cggaagtcac
      661 gcggtccgaa agctccacaa accccgcttc gccggtgtta aagaaaatca gatcttcagc
      721 aaaacgcgac agatgcacca tgccgatagc ggcagcagaa agcagttcca acacatggtc
      781 acggtcagaa acgctgtcca gactgttacg ggtcgccgaa gcaaagccca gccagcctgc
      841 taactgttca cggtcgattt cataggccgt tcccgccagc gcgccacagc ctaatgggct
      901 gacatccaga cgcttaagcg catcctgcaa acggctttca tcacgcgcca gcatctcaac
      961 ataggccagg caccagtgcg cgaacgtcac cggctgggcg cgttgcaggt gagtgtaacc
     1021 tggcattacc gcgtcctgat tgttttgtgc ggtttccacc agcgccgatt gcagctgccg
     1081 gttagccgtc agtaactcgc taacggtatc tttgcaccac agtttcaggt cagtcgctac
     1141 ctgatcatta cggctacgcc cggtatgcag ctttttgcct aactggccca ctttgtcgat
     1201 cagtttgcct tccacccagc tatggatatc ttcggcgtcg ctttcaagga tttgttgtgg
     1261 cctggcgcga acatcttcca gcaacacgtt cagcgcctct tccagttgcg cctgctcttc
     1321 tgcagttaac acgccgaccg ttaccaaggc tttggaccag gccacagagc caacaatatc
     1381 ctgctccgcc agacggtaat caaagcgcag tgagtcgttg aattgtttga accgttgatc
     1441 tgctgcctgg gtaaaacgcc cgccccaaag tgccataact ctgtttcctt attttgaaat
     1501 tcaatgccgg aaagctgcgc ctccggctaa caaaacttaa gctaaaatcc gcgtacccat
     1561 cggcataccg ttaaacagtg ccggaagctg ctccgcataa cgccaagaag cgatatctac
     1621 cggacggccc agcgtgcggg ccgcatccag cgccgcgttc actttcacta tcatgccgtc
     1681 agtaataatg ccctgctcaa tcagttgttc tgctttcgcg gcggtcattt cggcaatgcg
     1741 ttgccctttg ccgtcgagaa tgccgctgac gtcggagagc aaaatcagat ccgcgcccag
     1801 cgttgccgcc agcgccgttg ccgcctggtc ggcattgacg ttcatcagtt gcccttcgtc
     1861 tgttacgcca atggagctga ccaccggcag ataaccgttc tccagcaagg agttgataag
     1921 cttaggcgaa cctggctgcg ccagtccaac atgacctaac tcttcatcaa gctgggtcac
     1981 tttgacgctg tcgccgtcac cgagaaacaa acctacggcc gcaatctgat gtttcttcgc
     2041 ccatgccaac agggttttat ttgccgttcc cgccagtgct ccggtgataa tgtctatctg
     2101 atcagcaggc gtcacccgca ggccgttttt ctttttcacc ggcagattca gccctttcat
     2161 cagctcatcc accacgcaac cgccgccgtg cacaatcacc agcggacgct gatgtgactc
     2221 acgataattc accaatgcgc taaacagacg ttccagcgcc tcttcactat ccagcagtac
     2281 gccgcccagt ttgataatta atggattcat cattgcaccc ttaaataaga gactgcgttt
     2341 ccgcatagcc gaaacgaata ttggcgcact gtaccgcttg tgccgccgcg cctttcagta
     2401 agttgtcttc ggtcgccaca ataatcagat gctcgccctg aacggcaaac ccgatatcgc
     2461 aaaatggcag cccaacgaca tttttcagcg ccggaacgcc tttgtcatac agccgcacca
     2521 gcggtttatg ggcatacgcc tgctgtaacg cttgcgcgac ttgcgcctgg gtcacacccg
     2581 atttcaggcg gcaggtaatg gtttcgagaa tgccgcgcgg gaaattgccc agatgtgggg
     2641 tgaagataac gtcagcaccg aggtgtgtgg cgatctctgg ttgatggcga tgagtaaaga
     2701 cgccatacgg ttgcaggcta acttcacaaa aactatttga aatcgctgcc ttacgtcctg
     2761 caccgctcac gccgctggtg gcgttgatca ccggccactg attgaggtca agaagatcgg
     2821 catcaatcaa cggtttcagc gccagctgtg ccgccgtcgg ataacagcct ggcaccgcaa
     2881 tcaaattcgc ttcttttaat ttattaccgc accactccgc cagaccgtag gctgcctgtt
     2941 ccagcagttc cgggtattga tgggtaaagc cgtaatattt ttcatagaag gtggcgtcgt
     3001 taacacgaaa cgcgccggaa aggtcgaaca ccacgcagcc cgcttcaaga aactgcggcg
     3061 ctaaatcgtg gctaacttca tgggcggtgg cgagaaacac tacgtccacc cctgggctaa
     3121 actcgctgat atccgacatc ggctgcaacg gcagctcaac gatgcctttt agctgcggat
     3181 gcaaatcgga gattaacttt cccgcatcat tgctttgcgc tgaaacagtc aaagcggtta
     3241 tgttcatatg cggatggcga tttacatagg tcactagctc tgcgccagcg tagccgctgg
     3301 cacccacaat cagcgtattc aacatcgggg ctattcacct tcttatgtct ggttgccagg
     3361 ttaaacgtaa aacattcacc ttacggctgg tgggttttat tacgctcaac gttagtgtat
     3421 ttttattcat aaatactgca tgaatattga tactatcatg accagaggtg tgtcaacaat
     3481 gaaaaacaaa ttaccgccat ttatcgagat ttaccgcgct ctgattgcca caccttcaat
     3541 aagcgctacg gaagaggcac tcgatcaaag caatgcagat ttaatcactc tgctggcgga
     3601 ctggtttaaa gatttgggct tcaatgtgga agtgcagcct gttccaggaa ctcgcaacaa
     3661 atttaatatg ctggccagtt acggacaggg ggctggcggc ttgttgctgg cggggcatac
     3721 cgatacggtg ccatttgatg acggtcgctg gacgcgcgat ccgtttacac tgacggagca
     3781 tgacggcaag ctttacggct taggcaccgc cgacatgaaa ggcttttttg cgtttatcct
     3841 tgatgcgcta cgcgatgtcg acgtcacgaa actgaaaaaa ccgctctaca ttctggcgac
     3901 tgctgatgaa gaaaccagta tggccggagc gcgttatttt gccgaaacta ccgccctgcg
     3961 cccggattgc gccatcattg gcgaaccgac gtcactacaa ccggtacgcg cacataaagg
     4021 tcatatctct aacgccatcc gtattcaggg ccagtcgggg cactccagcg atccagcacg
     4081 cggaattaac gctatcgaac taatgcacga cgccatcggg catattttgc aattgcgcga
     4141 taacctgaaa gaacgttatc actacgaagc atttaccgtg ccatacccta cgctcaacct
     4201 cgggcatatt cacggtggcg acgcttctaa ccgtatttgc gcttgctgtg agttgcatat
     4261 ggatattcgt ccgcagcctg gcatgacact caatgaactt aatggtttgc tcaacgatgc
     4321 attggctccg gtgagcgaac gctggccggg tcgtctgacg gtcgacgagc tgcatccgcc
     4381 gatccctggc tatgaatgcc caccgaatca tcaactggtt gaagtggttg agaaattgct
     4441 cggagcaaaa accgaagtgg tgaattactg taccgaagcg ccgtttattc aaacgttatg
     4501 cccgacgctg gtgctcggtc cgggttccat caatcaagct catcagcctg atgaatatct
     4561 ggagacgcgg tttattaaac ccacccgtga gttgattatc caggtaattc accatttttg
     4621 ctggcattaa aacgtaggcc ggataaggcg ctcgcgccgc atccggcacc gttgccaaac
     4681 tccggtgccg caataatgtc ggatgcgatg cttgcgcatc ttatccgacc tacacctttg
     4741 gtgttatgta tggcgatttt ttaacatttc cataagttac gcttatttaa agcgtcgtga
     4801 atttaatggc gtaaattcct gctatttatt cgtttgctga agcgatttcg cagcatttga
     4861 cgtcaccgct tttacgtggc tttataaaag acgacgaaaa gcaaagcccg agcatattcg
     4921 cgccaatgcg acgtgaagga tacagggcta tcaaacgata agatggggtg tctggggtaa
     4981 tatgaacgaa caatattccg cattgcgtag taatgtcagt atgctcggca aagtgctggg
     5041 agaaaccatc aaggatgcgt tgggagaaca cattcttgaa cgcgtagaaa ctatccgtaa
     5101 gttgtcgaaa tcttcacgcg ctggcaatga tgctaaccgc caggagttgc tcaccacctt
     5161 acaaaatttg tcgaacgacg agctgctgcc cgttgcgcgt gcgtttagtc agttcctgaa
     5221 cctggccaac accgccgagc aataccacag catttcgccg aaaggcgaag ctgccagcaa
     5281 cccggaagtg atcgcccgca ccctgcgtaa actgaaaaac cagccggaac tgagcgaaga
     5341 caccatcaaa aaagcagtgg aatcgctgtc gctggaactg gtcctcacgg ctcacccaac
     5401 cgaaattacc cgtcgtacac tgatccacaa aatggtggaa gtgaacgcct gtttaaaaca
     5461 gctcgataac aaagatatcg ctgactacga acgcaaccag ctgatgcgtc gcctgcgcca
     5521 gttgatcgcc cagtcatggc ataccgatga aatccgtaag ctgcgtccaa gcccggtaga
     5581 tgaagccaaa tggggctttg ccgtagtaga aaacagcctg tggcaaggcg taccgaatta
     5641 cctgcgcgaa ctgaacgaac aactggaaga gaatctcggc tacaaactgc ccgtcgaatt
     5701 tgttccggta cgttttacct cgtggatggg cggtgaccgc gacggcaacc cgaacgtcac
     5761 tgccgatatc acccgccacg tcctgctgct cagccgctgg aaagccaccg atctgttcct
     5821 gaaagatatt caggttctgg tttctgaact gtcgatggtt gaagcgaccc ctgaactgct
     5881 ggcgctggtt ggcgaagaag gtgccgcaga accgtatcgc tatctgatga aaaacctgcg
     5941 ttctcgcctg atggcgacac aggcatggct ggaagcgcgc ctgaaaggcg aagaactgcc
     6001 aaaaccagaa gggctgctga cacaaaacga agaactgtgg gaaccgctct acgcttgcta
     6061 ccagtcactt caggcgtgtg gcatgggtat tatcgccaac ggcgatctgc tcgacaccct
     6121 gcgccgcgtg aaatgtttcg gcgtaccgct ggtccgtatt gatatccgtc aggagagcac
     6181 ccgccatacc gaagcgctgg gcgagctgac ccgctacctc ggtatcggcg actacgaaag
     6241 ctggtcagag gccgacaaac aggcgttcct gatccgcgaa ctgaactcca aacgtccgct
     6301 tctgccgcgc aactggcaac caagcgccga aacgcgcgaa gtgctcgata cctgccaggt
     6361 gattgccgaa gcgccgcaag gctccattgc cgcctacgtg atctcgatgg cgaaaacgcc
     6421 gtccgacgta ctggctgtcc acctgctgct gaaagaagcg ggtatcgggt ttgcgatgcc
     6481 ggttgctccg ctgtttgaaa ccctcgatga cctgaacaac gccaacgatg tcatgaccca
     6541 gctgctcaat atcgactggt atcgcggcct gattcagggc aaacagatgg tgatgattgg
     6601 ctattccgac tcagcaaaag atgcgggcgt gatggcagct tcctgggcgc aatatcaggc
     6661 acaggatgca ttaatcaaaa cctgcgaaaa agcgggtatt gagctgacgt tgttccacgg
     6721 tcgcggcggt tccattggtc gcggcggcgc acctgcccat gcggcgctgc tgtcacaacc
     6781 gccaggaagc ctgaaaggtg gcctgcgcgt gactgaacag ggcgagatga tccgctttaa
     6841 atatggtctg ccagaaatca ccgtcagcag cctgtcgctt tacaccgggg cgattctgga
     6901 agccaacctg ctgccaccac ctgagccgaa agagagctgg cgtcgcatta tggatgaact
     6961 gtcagtcatc tcctgcgatc tctaccgtgg ctacgtacgt gaaaacaaag attttgtgcc
     7021 ttacttccgc tccgctacgc cggaacaaga attgggcaaa ctgccgttgg gttcacgtcc
     7081 ggcgaaacgt cgcccaaccg gcggcgtcga gtcgctgcgc gccataccgt ggatttttgc
     7141 ctggacgcaa aaccgcctga tgctccccgc ctggctgggt gcaggtacgg cgctgcaaaa
     7201 agtggtcgaa gatggcaaac agagcgagct ggaagccatg tgccgcgatt ggccattctt
     7261 ctcgacgcgt ctcggcatgc tggagatggt cttcgccaaa gcagacctgt ggttggcgga
     7321 atactatgat caacgtctgg tagacaaagc actgtggccg ttaggtaaag agttacgcaa
     7381 cctgcaagaa gaagacatca aagtggtgct ggcgattgcc aacgattccc atctgatggc
     7441 cgatctgccg tggattgcag agtctattca gctacggaat atttacaccg acccgctgaa
     7501 cgtattgcag gccgagttgc tgcaccgctc ccgccaggca gaaaaagaag gccaggaacc
     7561 ggatcctcgc gtcgaacaag cgttaatggt cactattgcc gggattgcgg caggtatgcg
     7621 taataccggc taatcttcct cttctgcaaa ccctcgtgct tttgcgcgag ggttttctga
     7681 aatacttctg ttctaacacc ctcgttttca atatatttct gtctgcattt tattcaaatt
     7741 ctgaatatac cttcagatat ccttaaggaa ttgtcgttac attcggcgat atttttttca
     7801 agacaggttc ttactatgca ttccacagaa gtccaggcta aacccctttc tagctggaaa
     7861 gccctgggtt gggcactgct ctacttttgg tttttctcta ctctgctaca ggccattatt
     7921 tacatcagtg gttatagtgg caccaacggc attcgcgact cgctgttatt cagctcgctg
     7981 tggttgatcc cggtattcct ctttccgaag cggattaaaa ttattgccgc agtgatcggc
     8041 gtggtgctat ggacggcctc tctggcggcg ctgtgctact acgtcatcta cgggcaggag
     8101 ttctcgcaga gcgttctgtt tgtgatgttc gaaaccaaca ccaacgaagc cagcgagtat
     8161 ttaagccagt atttcagcct gaaaattgtg cttatcgcgc tggcctatac ggcggtggca
     8221 gttctgctgt ggacacgcct gcgcccggtc tatattccaa agccgtggcg ttatgttgtc
     8281 tcttttgccc tgctttatgg cttgattctg catccgatcg ccatgaatac gtttatcaaa
     8341 aacaagccgt ttgagaaaac gttggataac ctagcctcgc gtatggagcc tgccgcaccg
     8401 tggcaattcc tgaccggcta ttatcagtat cgtcagcaac taaactcgct aacaaagtta
     8461 ctgaatgaaa ataatgcctt gccgccactg gctaatttca aagatgaatc gggtaacgaa
     8521 ccgcgcactt tagtgctggt gattggcgag tcgacccagc gcggacgcat gagtctgtac
     8581 ggttatccgc gtgaaaccac gccggagctg gatgcgctgc ataaaaccga tccgaatctg
     8641 accgtgttta ataacgtagt tacgtctcgt ccgtacacca ttgaaatcct gcaacaggcg
     8701 ctgacctttg ccaatgaaaa gaacccggat ctgtatctga cgcagccgtc gctgatgaac
     8761 atgatgaaac aggcgggtta taaaaccttc tggatcacca accagcagac gatgaccgcc
     8821 cgcaatacca tgctgacggt attttcgcgc cagaccgaca agcagtacta catgaaccag
     8881 caacgtacgc agagtgcgcg tgaatacgac accaacgtgc tgaagccgtt ccaggatgtg
     8941 ctgaatgacc ctgcgccgaa gaaactgatc atcgttcatc tgctgggtac gcatatcaaa
     9001 tacaaatacc gctacccgga aaatcagggc aagtttgatg gcaataccga tcatgttccg
     9061 ccaggattaa gcgcagaaga gctggaatca tataacgatt atgacaacgc taacctgtat
     9121 aacgatcatg tggttgccag cctgattaaa gactttaaag cggcagaccc gaacggattc
     9181 ctggtttact tctctgacca cggtgaagag gtttacgaca cgccgccgca caaaactcag
     9241 gggcgtaacg aagacaaccc gacgcgccac atgtacacca ttccgttcct gctgtggacg
     9301 tcggaaaaat ggcaagcgac tcatccccgt gatttctcac aggatgtcga tcgtaaatac
     9361 agcctggcgg aactgatcca cacctggtca gatttggcgg gcttatctta cgacggttac
     9421 gatccaaccc gttcagtggt gaatccgcag ttcaaagaaa ctacccgctg gattggtaac
     9481 ccgtacaaga aaaacgcgct gatcgattac gacactctgc cgtatggcga tcaggtgggt
     9541 aatcagtaga tgacctttgt atcgcgtctg gcgctatttg ccggatgcga tactgccgca
     9601 atgtttacat cgaactcccc tcaggccaca cttttcaggt ggcctttttt aataatgcca
     9661 gttactgttt tcccgtctac atacctaagg gaataacatg tatcacgacg tcagctatct
     9721 gctctcccgc ctgatcaacg gcccgctgtc cctgcgccag atttactttg ccagcagtaa
     9781 cggcccggtg cccgatcttg cgtatcaggt cgattttcca cggctggaaa ttgtgctgga
     9841 aggtgagttt gttgataccg gcgctggagc aacgttagtt cccggcgatg tgctgtacgt
     9901 tgccgctggt ggctggaatt tcccacaatg gaaaacaccc gctaccacct ttagcgtgct
     9961 gtttggcaaa cagcaactcg gcttcagcgt cgtgcaatgg gatggcaaac aatatcaaaa
    10021 tctggcgaag caacacgtcg cccgacgcgg cccacgcata ggttcttttc tgctacaaac
    10081 gctcaatgaa atgcaaatgc agccgcagga gcagcaaacg gcaaggctta tcgtagccag
    10141 cctgcttagc cactgccgcg atttgctcgg cagccaaatc cagaccgcct cacgcagcca
    10201 ggcactattt gaagctattc gcgattatat cgacgaacgc tatgcctccg cgcttacccg
    10261 cgaatctgtc gcacaggcat tctatatttc accaaactat ctctcgcacc tgtttcaaaa
    10321 aacgggggcc attggtttta acgaatacct gaatcacacg cgactggagc acgctaagac
    10381 gttattgaaa ggctatgacc tgaaggtaaa agaagtggcg cacgcctgcg gctttgtcga
    10441 cagcaactac ttctgccgtc tgtttcgtaa aaacaccgaa cactcgccgt cggaatatcg
    10501 ccggcagtac cacagccaac tgacagaaaa accgactact ccagaataag atgggtttgc
    10561 tgcggcgcag aaagcacttt gcgcaccgcg ctcattaccc gctgcggctc acgcaggaat
    10621 gcgtagatgc tgcattgcac atagcggcaa tgttcaaatc gctcggcacc ggcaagctcg
    10681 atatccgtaa tcaacagagc aacatccgcc cgacggatat cctcgtccgc taaacgattc
    10741 tccgttccca gcgcgccctg agtttcaatg ctgacgcccc acttctctaa ctggcacaac
    10801 ttttccagcc gttccgccgc catataagta tgcgccacgc cgcttacgca ggcggttact
    10861 gccaccaggt atgccatttt aacctcccac ggtaacctga aatccggccc gttctgccat
    10921 ttcgcgcatc gttgtcacat cggctgacga cggcgcaggc acctctttca tcgaccatgt
    10981 tttccccagc aggcggtatt tcggttcgcc gtactgatga aacggtaaca gatggatctg
    11041 cctgattttc agcgggatca gtacatccag cgcctgctgc atattctccc ggctgagcgt
    11101 gaaaccaggg atcaacggta aacgcgggat cacgttgaca ccctcactca ccagcaaacg
    11161 cagattctcc agcacgcgtg gcaggttcat cttcaccaca tcccgcgcct gagtcgcgtc
    11221 cataattttt aaatcgaaca gcacttcatc gcacaatttc gccagcggta acagcttgct
    11281 tgctggcgcg tctccggcag tttcaatggc gcatgagaca ccccacagcc gcagtcgctg
    11341 taaaaaacgg gtagcaaact ccgcctgcat taacacttcg ccgccagaaa gcgtgacgcc
    11401 gccgccggac gtgcgaaaga aaatgtcatc tttcatcact tcccgttcca gagcgtcaag
    11461 gctgatatcg cgaccaatcc gttcaaacgc cccggagggg cattcatccg catcacgcaa
    11521 acatttcgca cagtgcagac atttcgcctc tctgcgtacc gtctggattt tgccggagat
    11581 cgactccgga ttagcacacc acgggcaaag atgcggacag cctttaaaaa agaccaccgt
    11641 acgaatgccc tcgccgtcat tcagtgaata acgctgaatg ttgaaaatgc gcgccacatc
    11701 atcgcggcgc gtttccacaa cgttacagct gatgcgctgt ccggcggatg atgtcatcct
    11761 ggatctcctt cgacagttcg acaaagaagg cgctgtatcc ggcaacgcgc accaccagcc
    11821 cggcataatc ttgtgggcgc tgttgcgctt cccgcaacgt gtcggcgttc accacgttaa
    11881 actgaatatg ctgcaactta agctgggtaa acacccgtaa gaagtcggcc agtttgcgta
    11941 atcccgcttc accttccagg gtcgccggag tgaatttcac gttcagcaac gtaccgttag
    12001 acagcagcgt attatcgagc ttactgactg acttcagtac cgccgttggc ccttgtgcgt
    12061 cctgacccag cataggtgac aagccgccgt ctgccagttg ttccccggca aaacgaccgt
    12121 ctggcgtcgc gccaaccacc gatcccaacg gaacgtgagc agaaacggta tacgatcccg
    12181 gcgtgaagta accgccgcgc gggttctggt atttttccac ttctttgcag tagtggcgca
    12241 acagttcggc gctaatgtta tccacctcgt cgatatcgtt accgtatttc tcaaagcggt
    12301 taattaagcg agcgcggact ttttcgcctt ctggcgttgc aaagttggct tttaataccg
    12361 acagcaattc gtcaaaactt aaacgctgtt gctcaaaaac catccctttc aacgcatgga
    12421 gagaatcgct caggttggcg ataccaattc cctgtacgcc ggagaagtta taacgcgcgc
    12481 cgccatcggt aatatcgcga cctttttcca gacaatcgct gataaaagac gagagcagcg
    12541 gtacaggtgc ccagtcgcga tggccgatat cacaaatatt gctgccctca accatcaggg
    12601 tgatgtagtg gctgatcttg gcgcggatct gctccagtaa accttcataa gtcagcgcag
    12661 cattgccttc attttcatgc aggcagattt ccatcacttt cagcagatta aacatcgcga
    12721 tgtcatgcaa gccgtaggtt ctgccgggaa tagataattc cacacagccc actacggaat
    12781 agtcgcgcgc atcttccagc gaaacgccac ggttgaggaa cgctggcacc accacttcat
    12841 cgttaaagat ttgcggaata ccggtgccga ggcgaatggt ttcggcggtt ttcatcagga
    12901 acggcgtgtc gatcaaggcg ttagtgcgca cgccgaggtt cggttgcggt aattgcacgc
    12961 tttgataggt gtcaaggcaa aggaacgaaa gcacgttcac cgcgctacgt ccgttctcgg
    13021 ttaacccgcc gagcagtgcg gtatagccgg tcgggaaacc ggcgaaataa cgcgcgctac
    13081 tggtggagcg caacagcacg atgtcgttgc atttcaccca taaagattcg agcagttctt
    13141 tcaggaacgc cggatcttcg ccctgggtta aagatgcctg atagaacggc aacatatact
    13201 ggtcgaagcg ccctaaagac agcgaactgg cgttggattc gtattgcaga atgatgttca
    13261 tgtaccagaa taactggcac gcctgccaga acgtctgcgg cttatgttgc gcgttatggc
    13321 gggagatctc cgcaatagtc agcagctctt cgcgacgctg ggcatctgtg cagtttgccg
    13381 ccatcgtttc cgccagttcg gcgtaacgca aaatgtgttt ctgcgaggct tccagcagta
    13441 acaacgcggc ctgataaaag tgattctccg gctgttgctg acaatgttgc tgcatctgtg
    13501 ccaccagctc acccagaccg tgattcaaca ggcgtgggta atcaataata atgtgcccct
    13561 ggcctttatc cgtctggttg atgctgaaaa tctgcgtgtt ggtcgcggct tttacttcat
    13621 ctgtcatctg cccgttgatg aaatctttca tcgaacgttt ttcccagtac gggaacaact
    13681 cttcacggta gatacgttta tcttcttcgc tgatagcaaa gcggtcctgc ggacgcgtcg
    13741 ggaattgatc cagctctttc agcagccagt aagggtccat ttccggcgac ataatcccgg
    13801 cgcgcggttt tacggtgcgg ttaccggcaa tcagttcttc atcacgaatc gaaatttcaa
    13861 catgttcaag gatatacgct gttgctttcg cccggcgcaa tatcaccggt tcgccttcgg
    13921 tttgccgatg gctggcggta taaagcagcg cccgctccag cgagatttca cgggtattgg
    13981 caaacagtgc agttttgagg cgagagatac gattcgtcat gaggaactcc tgtgggaaag
    14041 cgaacccgcc cttgcgagcg ggagaagaac ttaagcggtt tgcgccaggt gagcttcaat
    14101 tttgctcata atggcgtcag cacgttttac tgcatcgctg atgttgacgc gcacgatggt
    14161 cttgccagca aaacgctctt caaacttgat gccgatatct ttggtcagaa tcaccatatc
    14221 agcgctcgcc acgtcttccg cagtcagttc attttccaga ccaattgacc cctgggtttc
    14281 tactttcact tcccagcctt tcgctttcgc ggcactttcc agcgcctctg ctgccatata
    14341 ggtatgggca acacctgaag gacatgcggt tactgcaata attttcgtca tggctcgttc
    14401 ctcaattaat taatttcaaa atccaaatcc aggtcgtctt ctttttcatc agtcgaagac
    14461 ccatttttac gcgccagact tttcagcacg ttcacacaaa cagccgtcac cactgctccg
    14521 acagccactg ctgcgatata acccaactta ccttcaacca ccggcagcac aatcagacca
    14581 ccccagcctg catagcactg cgcaccgacc agcgccgcag ttactgcacc acaaactgaa
    14641 ccgaccataa tggaaggaat gacacgcagc ggatcggcag cggcgaaagg aatcgcccct
    14701 tcagtaacgc caacacagcc catcaccagt gccgctttgc cggtttcgcg ctcttctgcg
    14761 gagaaatttt tacgaccaat cagcgtcgcc agccccatcc ccagcggcgg gatacaaata
    14821 cccacggcag caatggccac cacggtataa acaccctgag caacgcaaat cagcatgaag
    14881 gcataggcca ctttgttaac cggaccgccc atatcgaacg ccagcatcag ccccatgatc
    14941 accgccagca taacaatgct gccctgctgc atcccctgaa gccactgagt caggctgttg
    15001 gtcaacgccc ctacaggctc gcccaagccc cacatcatga tgcctgcggt aatcagtgtg
    15061 ccgatgatag gaataatgaa gatgggcatc accgagcgca gaactttatg caccggaatt
    15121 ttcttcaggt aatgcaccac gatgccgccg ataatcccgg cgatcagcgc accaaagaag
    15181 cccgcaccaa agctgttacc aacccaggca ccgatagcgc acggagccag cgcagaacgc
    15241 tctgcaatgg agtaaccaat gtaagcagcg aggaaaggca ccatcagcgt caaacctgca
    15301 acgccgatat caaacagttt tttcaaattc ggatcggcta cggcatccgg cactgcgcct
    15361 ttgccataca acatgacgga aaccgccagc aaaataccgc ccgataccac gaagggaatc
    15421 atgtgtgaaa cgcccgtcat taaatgctga cgggtatttt tcaggatctg caccaactca
    15481 ttcataagct gctcccgggc ttgcaaaagc ccatgtccat atttttgatg gaggaaacaa
    15541 taggcaatcg caggcggagc aaacagtgga taaaaaggcc atttactgga cttttgtaat
    15601 atcagtacaa aaatgcgatc cgcctcataa cttgcgataa tccggaagca tgccgcaaaa
    15661 cggcgtcttg tgagacgcgt cgcaaattga cggttacatt acatttgtcc agcttttggc
    15721 agatttgtca catggcaggc cgttaccgtg cgctcttatc ctgtaaacag aaaaccgtac
    15781 cgggagaaag gcaatggccc tgattgtgga atttatttgt gagctaccta acggcgtaca
    15841 tgcgcgtccg gcaagccacg ttgaaacgct gtgtaatact ttttcatctc aaattgagtg
    15901 gcataacctg cgtactgacc gtaaaggcaa cgccaaaagc gctctggcgc tgattggcac
    15961 cgatacgctg gtgggtgata actgccagct actgatttcc ggggccgacg aacaggaagg
    16021 gcaccagcgt ttaagccaat ggctgcgcga tgaattcccc cactgcgatg cgccgctggc
    16081 ggaagttaaa tctgacgaac tggaaccact gccggtttca ctgaccaatc tgaatccgca
    16141 aattatccgc gcccgcaccg tgtgcagcgg tagtgcaggc ggcattctga cgccgatctc
    16201 ttctttagat ctcaatgcgc tgagtaatct tcccgcagcc aaagacgttg acgccgagca
    16261 atccgcactg gaaaacggcc tgacgctggt actgaaaaac attgagtttc gtctgctgga
    16321 tagcgacggt gctaccagcg cgattctgga agcgcaccga tccctggctg gcgatacttc
    16381 cctgcacgaa catttactgg caggcgtcag cgccggatta agctgtgccg aagcgattgt
    16441 cgccagtgcg aatcactttt gcgaagagtt tgcccgttcc agcagcagat acctgcaaga
    16501 acgtgccctg gacgtacgcg acgtctgctt ccagttactc cagcaaatct acggtgagca
    16561 acgcttcccg gcaccgggca aactgacgca gtccgccatt tgtatggctg atgaactgac
    16621 ccccagccag ttcctcgaac tggataaaaa tcacctcaaa ggattgttac tgaaaagcgg
    16681 cggcaccacc tcacatacgg tgatccttgc ccgttcgttc aacattccga cgctggttgg
    16741 tgtggatatt gatgccctca ctccgtggca gcatcaaacg atttatatcg acggcaacgc
    16801 cggggcgatt gtggttgagc caggagaagc cgtagctcgt tattatcagc aagaagcccg
    16861 cgtacaggac gccctgcgtg agcaacagcg tgtctagctg acccaacaag cccgtaccgc
    16921 tgacggtatc cgcattgaaa ttgccgctaa catcgctcac tccgtggaag cgcaggccgc
    16981 attcggcaat ggtgcggaag gcgttggttt gttccgcact gaaatgctct atatggatcg
    17041 caccagtgca ccgggcgaaa gcgagctgta caacattttt tgtcaggcgc tggagtctgc
    17101 caacggacgc agcattattg tgcgcactat ggacattggc ggcgacaaac ccgttgatta
    17161 tctaaacatt cccgcagagg caaacccgtt cctcggttat cgcgccgtgc gtatttatga
    17221 agaatacgcg tcgttattta ccacacaact acgctcgatc ctgcgcgcct ccgctcacgg
    17281 cagcctgaaa atcatgatcc cgatgatctc ctcaatggaa gagatcttat gggtgaaaga
    17341 aaaactggca gaagccaaac agcaactacg taacgaacac attccgtttg atgagaagat
    17401 ccagctcggc attatgctgg aagtaccgtc ggtgatgttc atcatcgatc aatgctgcga
    17461 agagattgat ttctttagta ttggtagtaa tgacctgacg caatatctgc tggcagtgga
    17521 tcgcgataac gctaaggtta ctcgtcacta caacagcctg aatccggcat tcttgcgggc
    17581 gctcgattac gacgtgcagg cggtgcatcg ccagggtaaa tggattggtc tgtgcggtga
    17641 gctgggagcg aaaggttccg tgctgccgtt gctggtcggc ttagggctgg atgaactcag
    17701 catgagcgca ccatcaattc cggcggcgaa agcgcggatg gcgcaacttg atagccgtga
    17761 gtgccgcaag ttgctcaacc aggcaatggc ctgccgtact tcgctggaag tggaacacct
    17821 gctggcgcaa ttccgcatga cccaacagga cgcaccgctg gtcaccgccg agtgcatcac
    17881 gctggaaagt gactggcgca gcaaagaaga agtgctcaaa ggcatgaccg ataacctgct
    17941 gctggcgggc cgctgccgct atccgcgtaa actggaagcc gacttatggg cgcgcgaggc
    18001 cgttttctct accggtctgg gctttagttt tgccattcca cacagcaaat cagaacacat
    18061 tgagcaatcc accatcagcg tggcgcgtct gcaagcgccg gtgcgctggg gcgatgatga
    18121 agcgcaattc atcattatgt taaccctgaa caaacacgct gcgggcgatc agcacatgcg
    18181 cattttctcg cgcctcgctc gccgcatcat gcacgaagaa ttccgtaacg cgctggttaa
    18241 cgccgcctct gccgacgcta tcgccagcct gctgcaacat gaactggaac tgtaaaagga
    18301 aacatcatgg aactgtatct ggacaccgct aacgtcgcag aagtcgaacg tctggcacgc
    18361 atattcccga ttgccggggt gacaactaac ccgagcatta tcgctgccag caaggagtcc
    18421 atctgggaag tgctgccgcg tctgcaaaaa gcgattggtg atgaaggcat tctgtttgct
    18481 cagaccatga gccgcgacgc gcaggggatg gtggaagaag cgaagcgcct gcgcgacgct
    18541 attccgggta ttgtggtgaa aatcccggtg acttccgaag gtctggcagc aattaaaata
    18601 ctgaaaaaat agggtattac tacacttggc accgctgtat atagcgccgc agaagggtta
    18661 ttagccgcac tggcaggggc aaaatacgtt gctccgtatg ttaaccgcgt agatgcccag
    18721 ggcggggacg gcattcgtac ggttcaggag ctgcaaacgc tgttagaaat gcacgcgcca
    18781 gaaagcatgg tgctgacagc cagctttaaa acgccgcgtc aggcgctgga ctgtttactg
    18841 gcaggatgtg aatccatcac cctgccctta gatgtagcgc aacaaatgct caacacccct
    18901 gcggtagagt cagctataga gaagttcgaa cacgactgga atgccgcatt tggcactact
    18961 catctctaaa ggagcaatta tggaccgcat tattcaatca ccgggtaaat acatccaggg
    19021 cgctgatgtg attaatcgtc tgggcgaata cctgaagccg ctggcagaac gctggttagt
    19081 ggtgggtgac aaatttgttt taggttttgc tcaatccact gtcgagaaaa gctttaaaga
    19141 tgctggactg gtagtagaaa ttacgccgtt tggcggtgaa tgttcgcaaa atgagatcga
    19201 ccgtctgcgt ggcatcgcgg agactgcgca gtgtggcgca attctcggta tcggtggcgg
    19261 aaaaaccctc gatactgcca aagcactggc acatttcatg ggtgttccgg tagcgatcgc
    19321 accgactatc gcctcgaccg atgcaccgtg cagcgcattg tctgttatct acaccgatga
    19381 gggtgagttt gaccgctatc tgctgttgcc aaataacccg aatatggtca ttgtcgacac
    19441 caaaatcgtc gcgggcgcac ctgcacgtct gttagcagcg ggtatcggcg atgcgctggc
    19501 aacctggttt gaagcgcgtg cttgctctcg tagcggcgcg accaccatgg cgggcggcaa
    19561 gtgcacccag gcggcgctgg cactggctga actgtgctac aacaccctgc tggaagaagg
    19621 cgaaaaagcg atgcttgccg ccgaacagca tgtagtaacc ccggcgctgg agcgcgtgat
    19681 tgaagcgaac acctatttga gcggcgttgg ttttgaaagc ggcggctggc ggcggctcac
    19741 gcggtgcata acggcctgac cgctatcccg gacgcgcatc actattatca cggtgaaaag
    19801 tggcatttgg tacgctgacg cagctggtac tggaaaacgc gccggtggag gaaatcgaaa
    19861 ccgtagctgc acttagccat gcggtaggtt tgccaataac tctcgctcaa ctggatatta
    19921 aagaagatgt cccggcgaaa atgcgcattg ttgcaaaagc atcttgtgca gaaggtgaaa
    19981 ccatccacaa catgcctggc ggcgcgacgc cagatcaggt ttacgccgca ctgctggtag
    20041 ctgaccagta cggtcagcgt ttcctgcaag agtgggaata acctactcca aactcccggc
    20101 ttgtccggga gtttgaacgc aaaattgcct gatgcgctac gcttatcagg cctacgcaat
    20161 ctctgcaata tattgaattt gcgtgctttt gtaggccgga taaggcgttc actccgcatc
    20221 cggcactctg tgccagtaat ctgaaactcc cacgttgttc aggagttttt tatccgcttc
    20281 tggacatttt ctccacagaa caatcgttag cccggaaggt cgaatctgtg actcttattt
    20341 tcacggacga accagatgaa agcttcactg gcgctgctca gcctgctaac ggcatttacc
    20401 agccactcct taaaatctcc cgccgttcca ccaaccgtgg tacaaatcca ggccaatacc
    20461 aaccttgcca tcgcagacgg tgccagacag caaattggca gcacgctatt ttacgatccg
    20521 gcgtatatgc agcttaccta tcctggcggt gatgttccgc aagaacgcgg tgtatgttcc
    20581 gatgtggtga tccgcgcatt gcgcagccag aaggtcgatt tgcagaaact ggttcacgaa
    20641 gatatggcga agaattttgc tgagtacccg caaaaatgga agttaaagcg cccggacagc
    20701 aacatcgatc accgtcgggt gcctaatctg gaaacctggt ttagccgcca caacaaaaca
    20761 cgccccatca gtaaaaatgc cggtgactat caagcgggcg atattgtctc ctggcgatta
    20821 gataacgggc tggcgcatat tggggtggcg tcagatggct tcgcccgcga cggtacgccg
    20881 ctggtgatac ataacattgg cgcaggcgcg caggaagaag atgtgctgtt tagctggcgg
    20941 atggtggggc attatcgtta ttttgttaaa taagagatcg ccccacatga agaaaaaatt
    21001 cagatgcttt ttacactgac agcttctttt ttcttacggc tcaccagcgc cagtgccagc
    21061 acaatcagca caataccgct accttcaacg gcccccggat tctcaccgag cagccaccag
    21121 gaaaagagta cgccacaaac gggaacggcc agtgtgctta agctggcaat actggcaggc
    21181 aagtttttca gcacaaacag ccataagctc cacgctagcg ccgtcgccag aatcgcactg
    21241 taggccagcg cccagaacac ggtgggctgc cagtcaattt cacgttgcgg caccagtaaa
    21301 gcgaccacac tcatcaccag cgccgcatac agcatctgcc aggatgttaa cgataataaa
    21361 tccacgcgcg gatgacgggc gtataggcgt ttagcaacaa tcgcgctcgc cccccaacta
    21421 acaccggaga ggattgccag catggcactt ttcatcgaag agaaatcgag ttgccacggc
    21481 tgcaacacca aaaataagcc gaaagcggca atcagaatcg cgaaatattg cccacgtcgc
    21541 aggcgttcac cgagaaacaa cgcggcgaaa atcaccaccc agaacggcat ggtatagctc
    21601 aggatcgcca ccttccccgc acctccgctg accaacgccc actgcgccaa accaaccatc
    21661 ccgcaggttt gtaacagggc aatggctaag gtgtatttaa acggcgtcgg gcgcattccg
    21721 cgaccacgta ataaaaggac gatgaataaa acgagagcgc cgaaaatgca gcgtaaggcg
    21781 gtaaagtcga aggcaccgat gtaactggtg acttgcttca tgaaaatcca gctataactc
    21841 cagataagtg tgagcacaac caagccactg attgccagtg ggttgctctt tcctgcggca
    21901 gacatgagag atccagtgtg tagttttcct gtggacacta tacgccggta aagaaacttc
    21961 agcgactgcc tgaaagacag tcgctgaaga aggtcagatt acagcaggtc gaaacggtcg
    22021 aggttcatca ctttcaccca tgccgctacg aagtctttaa caaacttctc gtgggcatcg
    22081 ctgctggcgt aaacttccgc tactgcacgc aggacagagt tagaaccaaa taccagatcc
    22141 gcacggctgg ctgtgtattt cacttcgccg gtttcgcggt cacgtccttc gaacagctct
    22201 ttcgattcgt cagtcgcttt ccactcgtaa cgcatatcca gcaagttcac gaagaagtca
    22261 ttgctcaata cgccaacgcg gtcagtgaag acgccgtttt tgctgccatc gaagttggca
    22321 cccagtacac gcatcccgcc caccagcgca gtcatttccg gcgcggtcag cgtcagttgc
    22381 tgtgctttgt cgatcagcag tgactcggtg gtggaaacgt ccagacgagc gcgatagtta
    22441 cggaaaccgt cagcaattgg ctccagcagc tcaaacatct caatgtcagt ctgatcctga
    22501 cgcgcatcaa cgcgacccgg cgcaaacggt acatgaatgc tcaaacctgc ggcgcttgcg
    22561 gctttctcaa caccaaccac accagccagc acgatgatat ccgccagcga ggctttacca
    22621 gactctttct ggattttctc cagaacaggc agagcacgaa cggctgcggc gttcacatcc
    22681 cagtcgcgct gcggcattaa tgccagacgc gcaccgttgg caccaccgcg tttgtcgcca
    22741 ccacggaagg tagaagcaga tgcccaggcc accgatacca gctcactaac agacagacca
    22801 gaatccgcaa tcgcgaattt cagatcgata atgtcctgct cggtcgggtt gtagatcggc
    22861 tgcggcagcg gatcttgcca gatcagatct tctttcggca cttccggccc gatgtagcga
    22921 gatttcggcc ccatatccct gtgcgtcagt ttgaaccagg cacgggcaaa ggcttcgttg
    22981 aacgcctgcg gatcgttgag gaaacgacga gagatcttct cgaactcagg atcaaaacgc
    23041 agcgtcaggt cggtcaccag cattgtcggt ttacgtttct tcgacggatc gaacggatcc
    23101 gggataattt ccggtgcgtc taccgcttcg aactggattg ctccagccgg actgcgggtc
    23161 tgtacccact catacttgaa cagattctcg aagaaatagt tgctccactg ggtcggcgtc
    23221 tgggtccaga ctacttccag accagaggta atggcatctg cgccaacgcc gctgccgtaa
    23281 gtgctcgccc agcctaaacc ttgttcttca atcggtgcag cttctggatc aggacctacg
    23341 tttgatgtcg gaccggcacc gtgggtttta cccagcgtat gaccacccgc aatcagcgcc
    23401 acggtttctt cgtcgttcat gcccatgttg ccgaaggtcg cgcggatagc tgctgccgca
    23461 gaaagcggtt cgccgctgtg atccgggcct tccgggttaa cgtaaatcag gcccatctcg
    23521 gttgcaccca gcggtgcttt cgccagcgct tccggatgac ggtgagtcag ccaggctttt
    23581 tcatcacccc agttaacatc cagatccggt tcccagacgt cttcacgacc ggcaccaaaa
    23641 ccgaaggtac ggaagccgga gttttccagc gccacgttac ctgcgaggat aaacaggtcg
    23701 gcccaggaga ttttctgacc atatttctgt ttgattggcc acaacaggcg acgcgcttta
    23761 tcgaggctta cgttatccgg ccaggagttc agcggtgcaa aacgttgctg accacgaccc
    23821 gcgccgccgc gtccatcgat tgaacggtaa gtacctgcgc cgtgccaagc catacgaata
    23881 aacagaccgg cgtagctgcc ccagtcggct ggccaccacg gctgagattc tgtcagcagg
    23941 gctttcagat cttttttcag gccgtagtaa tctaatttgc tgaattcttt gcggtagtca
    24001 aagtcctcac ccagcgggtt agaacgatta gaatgttggt ttaacaggtc aacacggagt
    24061 tgatttggcc accagtcgcg agtggttgtg cccgcccccg cgctctggtc gtgaccgccc
    24121 tgatggaacg ggcatttgcc agtggctgtg gtgttatgga tatcgtctga cgtgctcatc
    24181 aatgtgctcc cctctacagt gttaccgtta cgatacacag cgttagagag aagttataat
    24241 tgaattaatc cacggatgcg atagttgaga tcttacatat tgttggttaa agagatgtag
    24301 atcaaattga tcgtaattaa gcttttttga ttatgtccga attcggacaa atgctttata
    24361 aaaaagggtt ttagcttcat atccttcaag taatgaggga gaaaacaagg ctgacaacct
    24421 tattttcccg ccctcatttc gaggcagcat tttgtgctct gtttaaaatt tgtgatcact
    24481 gtgtgatttt cacaaaagcc acactattta taaaccaggt cgaacaccca gcgtatggca
    24541 aatcgcgtaa ctcatttcag cgcggttaag cgtatagaag tggaaatctt tcactccttc
    24601 acggcttaaa atcttcacca tatccatggc aatattcgcg cccaccagtt tgcgggtttc
    24661 ggcatcgtca tccagcccgt caaacatttg cgccatccac gccggaatac gcacgttggt
    24721 catgtcggca aatttcttcg cctgtttaaa gttagatacc ggcaaaatgc ccgggatgat
    24781 ttccacatca atgcccgccg atacacagcg gtcacgaaaa cgcaggtagc tttcgacatc
    24841 gaagaagaac tgagtaatcg cgcggttggc tccggcatcc actttgcgtt tcagattaag
    24901 caaatccgcc tgagcgcttt ttgcttccgg gtgaacttcc ggatacgccg ccacggatat
    24961 atcgaaatct gccacttctt ttaacagcgt caccaggtca gaagcataca tttctggctt
    25021 accacttccc ggcggcagat cgccacgcag tgccacgata tgacgaatac cgttattcca
    25081 gtagtcgcgt gcaatggtgc gcagctcgtc gggcgtcgca tcaatgcaag taagatgcgg
    25141 tgccgcttcc agaccagtgc gatctttaat gcctttaata atgctgtgcg tacggtcgcg
    25201 ctcgccggag ttcgcgccat aggtcaccga tacaaacttc ggtttcaggc tgctaaggcg
    25261 atcgatggag ttccacaggg tctgctccat ttcactggta cgcggcggga aaaactcgaa
    25321 cgaaacatta atctgcccct ggacttctgc caggctctga ttcagggcat cccgctggct
    25381 ggcgtgaaaa aagctcatac cttacctcat caatcgcttg tagttatatg ttgtgttgtg
    25441 cacatctatc catttagacg tccagatgta aagatgaagg aaaagccgaa gggcgtcaac
    25501 cgaaaaatga ccaatgatgg tgaggaaaaa tcagggaaga tgaaaaaact tcaggatggt
    25561 aagaaaaaga aaatccgata tgcaaagcaa accggactgc aaatataaac taaccatgta
    25621 cttattgctt cttgccggat tcgtcgtaaa cgcttcatcc ggcctaattc tatgataatt
    25681 caatatattt cattagaaac catagacccg aaaacatgag taccgggcat tattaaattt
    25741 ctgaaattac aacaactgtg ccagccggtt gatatccgac tgaatcgccc cggcggtgac
    25801 gtcgcgccca gcgccaggcc cacggatcac cagcggatta tcgcggtacc agcggctttc
    25861 gatggcaaag acgttatcgc atggcagcag tgatgccagc ggatagtctt cacgcaccgc
    25921 ctccacgccc acacgcgctt tgccgttggc atcgaaacgc gccacgtagc gcagcaccag
    25981 ccccatttcg cgggccgctt ccagccgttg caccatctgc tcgttaagtt catcgccatt
    26041 ttcaaagaaa tggtcgatgc tgccgccttc gcaatgagca ggcaccagcg attccacacg
    26101 tacctgatcc ggttcaatgt tgtaacccgc ttcacgcgcc agaatcacca gcttgcgcat
    26161 tacgtctttg ccggagaggt cgtcacgcgg atcaggctcg gttaagccct gctgccacgc
    26221 ctgatccacc agctcggtaa acggcacgct accgtcgaat tgcaggaaca gccaggagag
    26281 cgtgccggag aagatcccac tgatcgacaa aatggtatcg ccgctgtcga tcagatcgcg
    26341 cacggtatgg ttgatcggca agcccgcacc aacggtggcg ttgtatagcc agtgacgccc
    26401 ggttttttcg aaggcgtcgt ggatctggcg atatttattg ctgtcgcttg ctcccgccag
    26461 tttgttggca ctgatgacgt ggaaaccgtg gctggcgaaa tcgagatact gatcagcaag
    26521 ctgctggctg gcggtaacgt ccaacaccac taaatcatca tacggatggg cgcgcatcca
    26581 caggaaaagc gactcttcat cctgctcgac cgcttcatcg ttgaagaagg ctaacgcgcg
    26641 gctggcgtcc agcccgtcat aactcaacag actgcggcgg ctgtccacca cgcctgccag
    26701 cacaaattca aagccggtac gcgccgaaag cgtgctctgc tcgcgggcga acagttccag
    26761 ccagcgagag ccgatattgc ctttgccgaa caataccagg ccgatgcgtt tttctgcgcg
    26821 gaagacggac tgatgcagcc cctgaatcag gctttcggtc gggccggtgc gcagtactgc
    26881 caccagactg atgccgtcat cggactgcca cgtaaactcg accggctggc ctttcagttg
    26941 ctgccagaag cggtggcaat gcagcgggtt acgggtgaca cccgccccga ccatcgccac
    27001 cagcgccagc ccctgacgca ggcgcagttc tccaggtaat cccgcttcgt cgaggatttt
    27061 cagcgcactg tcggccactt ctgaggtgta gcaaaattgc aacaactggc gatcgttatg
    27121 cacgccaacc gccagtggac gcacctgggc acgtttcagg atctggtcga tctctttatg
    27181 cgccagtttg aaatcctgac tggcgggcac ctgaaactca atcaaacaga catcatcgtg
    27241 gctggtgaca atacgcgcac cagtaccgga ggccagcacg cgttcaatgc gcgtggaacc
    27301 ttgatccggc gtgtagctac agcgcagctg caggtcgatt tcgctgccag aaactggctg
    27361 taaagtacgg gcgtgaagaa cgggagccgc cagacgcgcc agttcgctgg cctcatccag
    27421 acgcagcaac ggcagcaggc aggcatcttt cactttacgc gggtcggcac tgtatacccc
    27481 ggcgacgtcg ctccagatgg ttacacgaga aacacccgcc agcgcaccga tttgtgtcgc
    27541 ggaatagtcg gaaccgttac gccccagcaa caccgtttca ccggcgttgt tacggctgat
    27601 aaacccggtc accaccagac gtttgctcgg atgttgtacc agtagctgtt gcagcaacgg
    27661 gtaagaaagc ccttcatcaa cctgcggttg cgcagcgcgt tcagcgcgta aaaactcacg
    27721 ggcatcaagc caggccgctg gcagcccttg ttgattaagt accgcagaca tcaggcgcgc
    27781 cgaccacact tcaccgtggc ccaccacttc cgcatacacc gcatcgttaa taccactgtc
    27841 gagcaacgcc gccaggcgct caagatcgct gacaaaagcg ctaatgaggc tatcggcttc
    27901 ttcagcgggt agcagaccgc taatcagatc gcactgatag cgacgcagcg tttgttgaac
    27961 ctgatgcgca gagagacgat cggtctggct tagtttcaac cagttaatca actggttagt
    28021 ggtgctaccg gcggcggaaa ccaccatcat atcgtcaggc tgagagaact ccgccataat
    28081 gcccgcgaca cgcaaataac acttcacatc agccagacta ctgccaccaa atttatgcag
    28141 ctgacgacct ttcgcccctg cctgcgcaat cacactcatt tttacccctt gtttgcagcc
    28201 cggaagccat tttccaggtc ggcaattaaa tcttcgccat cttcaatacc ggtggagata
    28261 cgcagcagcg tctcggagat cccggcggca gcacgcgctt ctggtgccat gcctgcatgt
    28321 gtcatggttg cggcgtgaga gattaaactt tccactcccc ctaatgattc cgccagcgta
    28381 aacaacgaca gcccgcccag gaaacgacac agcgtctgct catcgccatc cagttcaaaa
    28441 ctcaacattg cgccaaagcc tttttgctga cgcgcggcaa tttcatgccc ctgattttcc
    28501 ggcagcgacg ggtgatacag ttttttcacc aacggctggg tttgcagata tttcacaatc
    28561 gcctgcgcgt tgcgctgcgc cagctccata cgcggcacca gcgttcgcaa cccgcgtagc
    28621 agcagatagc tgtcaaacgc gccgcccgtc acgccaatat tgtttgccca ccaggcaagt
    28681 tcagtgacaa cgtccgggtc tttagcaatc accacgccag ccaccacgtc agagtgaccg
    28741 ttcagatatt tcgtacatga atgcaacacc agatcggcac ccaatgccag cggattttgt
    28801 aatgccgggc ttaagaaggt gttatccacc acgctcaccg ccccgacttc ccttgccaga
    28861 tggcagattt tcgcaatatc cacgacgcgt aacaatggat tacttgggct ttctaccagt
    28921 accagtttgg gtttttctgc cagcgctgcc cgtaatgcct gttcatcgcc ttgatcaaca
    28981 aacaacacgc gatagcaacc gcgtttcgcc agactgtcga acaggcgata gctaccgccg
    29041 tagcagtcgt gcggcgcaac cagcagatcg ccaggtttca aaaagacggt cgttaccagg
    29101 tgaatcgcgg acatgccggt attagtaagt actgcgccag caccgccttc cagttctgcc
    29161 agcgcacgct gaaccacatc gcgcgttggg ttgccgcgac gcgagtaatc atgcgcgcgc
    29221 ggttcattaa atccggtaaa gttataggtg ctggaaagat ggatgggagg gacaacgcaa
    29281 ccatactgtt cgtcgtcatt taacccgcta cgcactgcga tggtggcctg tttacgcgtc
    29341 atgtgatgaa gtttcctggg ctttgtcggt gaaatgtcag gcaccagagt aaacattgtg
    29401 ttaatggacg tcaatacatc tggacatcta aacttctttg cgtatagatt gagcaaatcc
    29461 caaatagccg ttaaaattat atgcattatc acgccgacag gtgcattaca cgatgtcacg
    29521 gtaacgcctg tacggtaaac tatgcgggtt tacggtcagt acccacataa attgtggggt
    29581 ctggtctcaa tttattgacg aagaggatta agtatctcat ggctgaatgg agcggcgaat
    29641 atatcagccc atacgctgag cacggcaaga agagtgaaca agtcaaaaag attacggttt
    29701 ccattcctct taaggtgtta aaaatcctca ccgatgaacg cacgcgtcgt caggtgaaca
    29761 acctgcgtca cgctaccaac agcgagctgc tgtgcgaagc gtttctgcat gcctttaccg
    29821 ggcaaccttt gccggatgat gccgatctgc gtaaagagcg cagcgacgaa atcccggaag
    29881 cggcaaaaga gatcatgcgt gagatgggga ttaacccgga gacgtgggaa tactaaagcg
    29941 gaaaagagcg gcgcggagtg gaatcgcctg atgcactacg cttatcaggc ctacgtcata
    30001 ttgcaattta ttgaatttgc acgaacttgt aggccggata aggcgttcac gccgcatccg
    30061 gcataaacaa cgagcacgtt gtctgcgacc caccgctttt tatacatgga cgtttaacta
    30121 tgaaaaacag gctgctgatc ctcagcctgc tggtttctgt acctgccttt gcctggcagc
    30181 cgcaaactgg cgacatcatc tttcaaatct ctcgctcatc gcaaagtaaa gcgatccaac
    30241 tggcaaccca ttccgattac agccacaccg gtatgctggt gatgcgtaac aaaaagccct
    30301 acgtttttga agcggtcggc ccggtgaaat acaccccgct caagcagtgg attgcccatg
    30361 gtgaaaaggg caactacgtt gttcgccgcg ttgaaggcgg actgagtgtt gaacaacagc
    30421 aaaaactgac gcaaacggca aaacgttatc tcggtaaacc gtatgatttc agcttctcat
    30481 ggagcgacga tcgccagtac tgttcggaag tggtgtggaa ggtttaccag aacgcgctgg
    30541 gaatgcgcgt gggcgaacag cagaagttga aagagtttga tctcagcaac ccgctggtcc
    30601 aggcgaagct caaagaacgc tacggtaaaa atattccgct tgaggaaacg gtagtttcac
    30661 cacaagccgt tttcgacgcg ccacaactca ccacggttgc caaagagtgg ccgctgtttt
    30721 cgtggtgata cgaccaaaat tcaggcacaa aaaaagcgcc gtgcggcgct tttttcggaa
    30781 atccggtctt atttgctgcc cgggatgttg aaacgcttgt tgaagcggtc aacacggcca
    30841 ccggtagcaa catcacgctg tttgccagtg aagaacgggt ggcacttgct gcacacgtcg
    30901 aggttcaggt catgaccaac ggtggagcgg atttttatta cgttaccgca agagcagcta
    30961 gcagtaattt cttcgtattt cgggtgaata tcttttttca tgggaaaacc tcggtttaag
    31021 gccgcgtcgc tcttccagcc ctaacgccag acaccacgcg atgttaaaag tatagcttca
    31081 atacgatcat ttcgtacgaa ggcgcgaaat catacagaaa ttaaccagcg tatgcaaact
    31141 gatccgcact cttctacggc aatgtgtata ctaacccacc gaatttcaag tcaggatgat
    31201 gctatgcccg ttgcccacgt tgccttgccc gttccgcttc ctcgtacctt tgactatctg
    31261 ctgccagaag gcatgacggt taaagctggg tgtcgcatgc gcgtgccgtt tggcaaacag
    31321 caggagcgca tcgggattgt ggtatcagtt agcgatgcca gcgaactgcc gctcactgag
    31381 ctaaaagcgg tagtcgaagt gctggatggt gagccggtgt ttactcactc cgtctggcga
    31441 ttgctgctat gggcggcaga ttactatcat catccgattg gcgatgtgct gtttcatgcc
    31501 ttgccgattt tactacgcca ggggcggcct gcggcgaacg cgccgatgtg gtactggttt
    31561 gccactgaac aaggtcaggc ggtggatctg aacagcctga aacgctcacc caagcaacaa
    31621 caggcgctgg cagcgttacg gcaaggcaaa atctggcgcg accaggttgc cacgctcgaa
    31681 tttaatgatg ccgcgttgca ggcgctgcgc aaaaaaggtc tgtgtgattt agcaagtgaa
    31741 acaccagagt ttagcgactg gcgaacgaac tatgccgttt ctggtgagcg gttgcgattg
    31801 aataccgaac aggccaccgc cgttggcgca attcatagcg cggcagatac tttttctgcc
    31861 tggctgctgg cgggcgttac cggttccggt aaaacggagg tttatctcag tgtactggaa
    31921 aacgtgctcg ctcagggcaa acaggcgctg gtgatggtgc cggaaatcgg cctgacaccg
    31981 caaactatcg cccgttttcg tgaacggttt aatgcgcccg tggaagttct gcattccggt
    32041 ctgaacgaca gcgagcgtct ttcggcgtgg ctgaaagcga aaaatggcga ggcggcgatt
    32101 gtgatcggca cccgctccgc gctgtttacg ccgtttaaaa atctcggcgt gattgtcatt
    32161 gacgaagagc acgacagttc ctacaagcag caggaaggct ggcgctatca tgcccgcgac
    32221 ctggcggtgt atcgtgcgca tagcgagcaa atcccgatta ttcttggctc cgcaacgccc
    32281 gcgctggaaa cgttgtgcaa cgtccagcag aaaaaatacc gcctgctgcg cctgacgcgc
    32341 cgggcgggga atgcgcgtcc ggcaattcaa cacgtgctgg atttaaaagg tcagaaggtg
    32401 caggcaggtc tggctccggc gttaatcacc cgtatgcgcc agcatttaca ggccaacaat
    32461 caggttatcc tctttcttaa ccgccgtggc tttgcgcctg cgctactgtg ccacgactgc
    32521 ggctggattg ccgaatgccc acgttgcgat cactactaca cgctgcatca ggcgcaacag
    32581 catttgcgct gtcaccactg tgacagtcag cgtccagtgc cgcgccagtg cccttcctgc
    32641 ggttccacgc acctggtacc cgtggggctg ggcaccgaac agcttgaaca gacgcttgcg
    32701 ccgctgttcc ctgatgtgcc catttctcgt atcgaccgcg ataccaccag ccgcaaaggg
    32761 gcgctggaac agcaactggc ggaagtacat cgcggcggtg cgaggatttt gatcggcaca
    32821 caaatgctgg cgaaaggtca ccactttccg gatgtgacgc tggtggcgtt actggatgtg
    32881 gacggcgcgc tgttttctgc cgattttcgc tcggcagagc gtttcgctca gctttacacc
    32941 caggtcgctg gtcgtgccgg gcgtgcgggt aaacaaggcg aagtggtgct gcaaacgcac
    33001 catccggaac atcctctgtt gcaaacgttg ctctataaag gctacgacgc ctttgccgaa
    33061 caggcgctgg ctgagcgacg gatgatgcaa ctgccgccat ggaccagcca tgtgattgtg
    33121 cgtgcggaag atcataacaa tcagcatgcg ccgctgttcc tgcaacaact gcgtaatctg
    33181 atcctctcca gcccgctggc agacgacaaa ctgtgggttc tcggtccggt tccggcactg
    33241 gcacctaaac gtggcggtcg ctggcgctgg cagatattgt tgcagcaccc ttcccgcgtg
    33301 cgcttgcaac acatcattag tggtacgctg gcgctcatca atacaatacc ggattcccgc
    33361 aaggtgaaat gggtactgga tgttgatccg attgagggtt aaaccgctta cgatgcgagg
    33421 cggatcgaaa aattcaatat tcatcacact tttcatgaaa attctgtaac cgttttcacg
    33481 cgctatctgc taaaaatgtt gccgatgtga agtaaacatg gatgtagtac gcctgacgtg
    33541 ccaggcgagg agtgagtgtg aaagcgaaga agcaggaaac tgccgcgacc atgaaagacg
    33601 ttgccctcaa ggcaaaagtc tctacagcga ctgtctcccg agcattaatg aatcccgata
    33661 aagtctccca ggccacccgt aatcgggttg aaaaagcggc ccgggaagtg ggttatttac
    33721 cgcagcctat ggggcgcaac gtcaagcgta atgaatcccg caccattctg gtgattgtcc
    33781 cggatatctg cgatcccttc tttagcgaaa ttattcgcgg tatcgaagtt acggcggcaa
    33841 atcacggata tctggtgctg attggcgact gtgcacatca aaatcagcag gaaaaaacct
    33901 ttatcgattt gatcatcacc aagcaaattg atggcatgtt gctgctgggt tcaaggctgc
    33961 cgtttgatgc cagcattgag gaacagcgta atctgccgcc gatggtgatg gcgaacgaat
    34021 ttgcaccgga actggagctg cctaccgttc atatcgacaa tctgaccgcc gcatttgatg
    34081 cagtaaatta tttatatgag caagggcata aacggattgg ctgtatagcc ggtcccgaag
    34141 agatgccact gtgtcactac cgcctgcaag gctatgttca ggcgctgcgt cgctgcggaa
    34201 ttatggttga tccacaatac atcgcccgtg gcgacttcac cttcgaagcc ggaagcaaag
    34261 caatgcaaca gctgcttgat cttccacaac cgcctactgc agtcttctgc catagcgatg
    34321 tgatggcgct cggcgcactt tctcaggcaa aacgccaggg gctgaaagtc ccggaagacc
    34381 tttccataat cggttttgat aacatcgacc tgacgcaatt ttgtgatccg ccgctgacaa
    34441 ccatcgcgca gccgcgttac gaaatcggtc gggaagctat gctgttattg cttgatcaaa
    34501 tgcaggggca acacgttggc agtggctctc gtttaatgga ctgcgaactt atcatccggg
    34561 gatcaacacg cgcgttacct taaagtaaac ctttaagact gccgtgactg gtcaaaggcc
    34621 cgccgcttaa gtaacatggc gggctgacga acgaataaat acagcgaaac gatagtggca
    34681 caacgagatt atgtacgccg cagccaaccg gcaccttcgc ggcgaaaaaa gagcacctca
    34741 cggaaaaagc aacgaaatct gcctgcggtt tctcccgcta tggtcgctat tgctgccgcc
    34801 gttcttgtga cctttatcgg tggtctgtac ttcattacgc atcacaagaa agaagagtcc
    34861 gagacgctgc aaagccagaa agtgaccgga aacggactac caccaaaacc agaagaacgc
    34921 tggcgctaca ttaaagagct ggaaagtcgc cagccgggtg tgcgtgcgcc cacagaacct
    34981 tctgccggtg gtgaagtgaa aacgccggag caactgacac cagaacaacg tcagcttctt
    35041 gaacaaatgc aggctgatat gcgccagcag ccaacgcagc tggttgaggt tccgtggaat
    35101 gaacagacgc cggaacagcg tcagcaaacg ctacaacgcc agcgtcaggc gcagcaatta
    35161 gcggagcagc aacgcctggc tcagcagtcg cgtacgactg aacaaagctg gcagcagcag
    35221 acgcgtacgt cgcaagccgc gccggtacag gcgcaaccgc gccagtctaa accagcttct
    35281 actcagcagc cgtaccagga tctgctgcaa acccctgcgc acacgaccgc gcagtcgaag
    35341 ccgcagcaag ctgcgccggt tgctcgtgcc gctgacgcgc caaaaccgac ggcggagaaa
    35401 aaagacgaac gccgctggat ggtgcagtgc ggttcgttca gaggcgcgga acaggcagag
    35461 acggtacgtg ctcagctggc gttcgaagga tttgactcga aaatcaccac caacaatggc
    35521 tggaatcgtg tggtcattgg cccggtgaaa ggcaaagaga acgcagacag caccctcaat
    35581 cggttgaaga tggcgggtca tacaaactgc attcggctcg ccgccggggg ttgaaaccct
    35641 caaaatcccc cccatctata attgcattat gccccgtact tttgtacggg gtttgtactc
    35701 tgtattcgta accaaggggt cagctcgtga caactatagt aagcgtacgc cgtaacggcc
    35761 atgtggtcat cgctggtgat ggtcaggcca cgttgggcaa taccgtaatg aaaggcaacg
    35821 tgaaaaaggt ccgccgtctg tacaacgaca aagtcatcgc gggctttgcg ggcggtactg
    35881 cggatgcttt tacgctgttc gaactgtttg aacgtaaact ggaaatgcat cagggccatc
    35941 tggttaaagc cgccgttgag ctggcaaaag actggcgtac cgatcgcatg ctgcgcaaac
    36001 ttgaagcact gctggcagtc gcggatgaaa ctgcatcgct tatcatcacc ggtaacggtg
    36061 acgtggtgca gccagaaaac gatcttattg ctatcggctc cggcggccct tacgcccagg
    36121 ctgcggcgcg cgcgctgtta gaaaacactg aacttagcgc ccgtgaaatt gctgaaaagg
    36181 cgttggatat tgcaggcgac atttgcatct ataccaacca tttccacacc atcgaagaat
    36241 taagctacaa agcgtaagga tctcccatgt ctgaaatgac cccacgcgaa atcgtcagcg
    36301 aactggataa gcacatcatc ggccaggaca acgccaagcg ttctgtggcg attgctctgc
    36361 gtaaccgctg gcgtcgcatg cagctcaacg aagagctgcg ccatgaagtg accccgaaaa
    36421 atatcctgat gatcggcccg accggtgtcg gtaaaactga aatcgcccgt cgtctggcta
    36481 agctggcgaa tgcgccgttc atcaaagttg aagcgaccaa atttaccgaa gtgggctacg
    36541 tcggtaagga agttgattct attattcgcg atctgaccga tgccgccgtg aaaatggtac
    36601 gcgtccaggc tatcgagaaa aaccgttatc gcgctgaaga actggcagaa gaacgtattc
    36661 tcgacgtgct gatcccacct gctaaaaaca actggggaca gactgaacag cagcaggaac
    36721 cttccgctgc tcgtcaggca ttccgcaaaa aactgcgtga aggccagctt gatgacaaag
    36781 aaatcgagat cgatcttgcc gcagcaccga tgggcgttga aattatggct cctccgggca
    36841 tggaagagat gaccagccag ctgcagtcca tgttccagaa cctgggcggc cagaagcaaa
    36901 aagcgcgtaa gctgaaaatc aaagacgcca tgaagctgct gattgaagaa gaagcggcga
    36961 aactggtgaa cccggaagag ctgaagcaag acgctatcga cgctgttgag cagcacggga
    37021 tcgtgtttat cgacgaaatc gacaaaatct gtaagcgcgg cgagtcttcc ggtccggatg
    37081 tttctcgtga aggcgttcag cgtgacctgc tgccgctggt agaaggttgc accgtttcca
    37141 ccaaacacgg gatggtcaaa actgaccaca ttctgtttat cgcttctggc gcgttccaga
    37201 ttgcgaaacc gtctgacctg atcccggaac tgcaaggtcg gctgccaatc cgcgttgaac
    37261 tgcaggcact gaccaccagc gacttcgagc gtattctgac cgagccgaat gcctctatca
    37321 ccgtgcagta caaagcactg atggcgactg aaggcgtaaa tatcgagttt accgactccg
    37381 gtattaaacg catcgcggaa gcggcatggc aggtgaacga atctaccgaa aacatcggtg
    37441 ctcgtcgttt acacactgtt ctggagcgtt taatggaaga gatttcctac gacgccagcg
    37501 atttaagcgg tcaaaatatc actattgacg cagattatgt gagcaaacat ctggatgcgt
    37561 tggtggcaga tgaagatctg agccgtttta tcctataatc gcgttcaatc attttcatca
    37621 ttgtttgatg gggctgaaag gccccatttt tattggcgcg tattatgact gaacaacaaa
    37681 ttagccgaac tcaggcgtgg ctggaaagtt tacgacctaa aaccctcccc ctcgcctttg
    37741 ctgcaattat cgtcgggacg gcgctggcat ggtggcaagg tcacttcgat ccgctggtcg
    37801 ccctgctggc actaattacc gccgggctat tacagatcct ttctaacctc gccaatgatt
    37861 acggcgatgc ggtaaaaggc agcgataaac ctgaccgcat tgggccgcta cgcggcatgc
    37921 aaaaaggggt cattacccag caaaagatga aacgggcgct cattattacc gtcgtgctca
    37981 tctgtctctc cgggctggca ctggttgcag tggcatgcca tacgctggcc gattttgtcg
    38041 gtttcctgat tcttggcggg ttgtcgatca ttgccgctat cacctacacc gtgggcaatc
    38101 gtccttatgg ttatatcggt ctgggtgata tttccgtact ggttttcttt ggctggttga
    38161 gtgtcatggg gagctggtat ttacaggctc atacattgat tccggcactg atccttccgg
    38221 cgaccgcatg cggcctgctg gcaacggcag tactgaatat taataacctg cgtgatatca
    38281 atagcgaccg cgaaaatggc aaaaacacgc tggtggtgcg cttaggtgaa gtgaacgcgc
    38341 gtcgttatca tgcctgcctg ctgatgggct cgctggtgtg tctggcgctg tttaatctct
    38401 tttcgctgca tagcctgtgg ggctggctgt tcctgctggc ggcaccatta ctggtgaagc
    38461 aagcccgtta tgtgatgcgg gaaatggacc cggtggcgat gcgaccaatg ctggaacgta
    38521 ctgtcaaggg agcgttactg actaacctgc tgtttgtttt agggatattc ctaagccagt
    38581 gggcagcata actgacaaat atcaattaac aattgatgat tttgccaaca gcccacatag
    38641 cgcgatatac tgaaaattct cgcagcaact gaatgttaag cctatgaaat acgatacttc
    38701 cgagctttgt gacatctatc aagaagatgt taacgtcgtg gaaccgctgt tctccaactt
    38761 tggcggacgg gcgtcgtttg gcggacaaat aatcacggta aaatgtttcg aggacaacgg
    38821 gttgctgtac gatctgctcg aacagaatgg ccgtggtcgt gttcttgtcg tcgatggcgg
    38881 tggttctgtt cgtcgcgcac tggtcgatgc tgaactggcg cgtctggcag tacaaaatga
    38941 atgggaaggt ctggtcattt acggcgcggt gcgtcaggta gatgacctgg aagagttgga
    39001 tatcggcatc caggcgatgg cggcaattcc ggttggtgcc gctggcgaag gcattggcga
    39061 aagcgatgtc cgcgtcaatt ttggcggtgt caccttcttc tccggcgacc atctttatgc
    39121 cgacaatacc gggattattc tttcagaaga tccgctggat attgaatgat aagaaaggca
    39181 ccgcaaggtg ccttttttct gcgttacctg ttggcctaca cagtaaagaa attacgcgaa
    39241 agatgaagcg taatcagacc tcttccatgc gacccagcag ggcctgcaga cgttcctgcc
    39301 agccgttctg ctgttctttc agatggttgt tctcacgctc cagctcttcg cgctgatgct
    39361 gggcattttg aacttcctgc gacagtgagt tgtttttttc tttcagctct tcgatttcca
    39421 tctgcaacag agtgatggta tcaatcgcct gctgtacttt tgcttccagt ttctcaaaca
    39481 cttctaatga cattgtcata cctctcctga attgcaaggc gttgatggat aaaaaatcct
    39541 cgtcccgatt accggtgacg ccttaataaa tacgagcgca ctttagttag ctccgattgt
    39601 atgaagccgc gccatcgctg tccagcggca cgccttgcag attacggttt gccatacttt
    39661 tcatccttct cctagtgaca taatccacat caatcgaaaa tgttaataaa tttgttgcgc
    39721 gaatgatcta acaaacatgc atcatgtaca atcagatgga ataaatggcg cgataacgct
    39781 cattttatga cgaagcacac acattttaag ttcgatattt ctcgtttttg ctcgttaacg
    39841 ataagttaac agcatgccta caagcatcgt ggaggtccgt gactttcacg catacaacaa
    39901 acattaactc ttcaggatcc gattatgagt caaacatcaa ccttgaaagg ccagtgcatt
    39961 gctgaattcc tcggtaccgg gttgttgatt tttttcggtg tgggttgcgt tgcagcacta
    40021 aaagtcgctg gtgcgtcttt tggtcagtgg gaaatcagtg tcatttgggg actgggggtg
    40081 gcaatggcca tctacctgac cgcaggggtt tccggcgcgc atcttaatcc cgctgttacc
    40141 attgcattgt ggctgtttgc ctgtttcgac aagcgcaaag ttattccttt tatcgtttca
    40201 caagttgccg gcgctttctg cgctgcggct ttagtttacg ggctttacta caatttattt
    40261 ttcgacttcg agcagactca tcacattgtt cgcggcagcg ttgaaagtgt tgatctggct
    40321 ggcactttct ctacttaccc taatcctcat atcaattttg tgcaggcttt cgcagttgag
    40381 atggtgatta ccgctattct gatggggctg atcctggcgt taacggacga tggcaacggt
    40441 gtaccacgcg gccctttggc tcccttgctg attggtctac tgattgcggt cattggcgca
    40501 tctatgggcc cattgacagg ttttgccatg aacccagcgc gtgacttcgg tccgaaagtc
    40561 tttgcctagc tggcgggctg gggcaatgtc gcctttaccg gcggcagaga cattccttac
    40621 ttcctggtgc cgcttttcag ccctatcgtt ggcgcgattg taggtgcatt tgcctaccgc
    40681 aaactgattg gtcgccattt gccttgcgat atctgtgttg tggaagaaaa ggaaaccaca
    40741 actccttcag aacaaaaagc ttcgctgtaa tatgactacg ggacaattaa acatgactga
    40801 aaaaaatata tcgttgcgct cgaccagggc accaccagct cccgcgcggt cgtaatggat
    40861 cacgatgcca atatcattag cgtgtcgcag cgcgaatttg agcaaatcta cccaaaacca
    40921 ggttgggtag aacacgaccc aatggaaatc tgggccaccc aaagctccac gctggtagaa
    40981 gtgctggcga aagccgatat cagttccgat caaattgcag ctatcggtat tacgaaccag
    41041 cgtgaaacca ctattgtctg ggaaaaagaa accggcaagc ctatctataa cgccattgtc
    41101 tggcagtgcc gtcgtaccgc agaaatctgc gagcatttaa aacgtgacgg tttagaagat
    41161 tatatccgca gcaataccgg tctggtgatt gacccgtact tttctggcac caaagtgaag
    41221 tggatcctcg accatgtgga aggctctcgc gagcgtgcac gtcgtggtga attgctgttt
    41281 ggtacggttg atacgtggct tatctggaaa atgactcagg gccgtgtcca tgtgaccgat
    41341 tacaccaacg cctctcgtac catgttgttc aacatccata ccctggactg ggacgacaaa
    41401 atgctggaag tgctggatat tccgcgcgag atgctgccag aagtgcgtcg ttcttccgaa
    41461 gtatacggtc agactaacat tggcggcaaa ggcggcacgc gtattccaat ctccgggatc
    41521 gccggtgacc agcaggccgc gctgtttggt cagttgtgcg tgaaagaagg gatggcgaag
    41581 aacacctatg gcactggctg ctttatgctg atgaacactg gcgagaaagc ggtgaaatca
    41641 gaaaacggcc tgctgaccac catcgcctgc ggcccgactg gcgaagtgaa ctatgcgttg
    41701 gaaggtgcgg tgtttatggc aggcgcatcc attcagtggc tgcgcgatga gatgaagttg
    41761 attaacgacg cctacgattc cgaatatttc gccaccaaag tgcaaaacac caatggtgtg
    41821 tatgtggttc cggcatttac cgggctgggt gcgccgtact gggacccgta tgcgcgcggg
    41881 gcgattttcg gtctgactcg tggggtgaac gctaaccaca ttatacgcgc gacgctggag
    41941 tctattgctt atcagacgcg tgacgtgctg gaagcgatgc aggccgactc tggtatccgt
    42001 ctgcacgccc tgcgcgtgga tggtggcgca gtagcaaaca atttcctgat gcagttccag
    42061 tccgatgttc tcggcacccg cgttgagcgc ccggaagtgc gcgaagtcac cgcattgggt
    42121 gcggcctatc tcgcaggcct ggcggttggc ttctggcaga acctcgacga gctgcaagag
    42181 aaagcggtga ttgagcgcga gttccgtcca ggcatcgaaa ccactgagcg taattaccgt
    42241 tacgcaggct ggaaaaaagc ggtcaaacgc gcgatggcgt gggaagaaca cgacgagtaa
    42301 tgtcaggcac ctgaatcgat tgcctgatgc tgatcgcctg atgcgacgct tacgcgtctt
    42361 atcaggccta cgagccaatg ccattttgtg taggcgggat aaggcgttca cgccgcatcc
    42421 ggcatcacgc agactctgaa accgtgcggg ggcaaccccg cacacatcaa taatcccacc
    42481 cttcccctgt gctacacttc gcgccattcc ttactgctta gagtttgcta tgagacgaga
    42541 acttgccatc gaattttccc gcgtcaccga atctgcggcg ctggctggct acaaatggtt
    42601 aggacgcggc gataaaaaca ccgcggacgg cgcggcggta aacgccatgc gtattatgct
    42661 caaccaggtc aacattgacg gcaccatcgt cattggtgaa ggtgaaatcg acgaagcacc
    42721 gatgctctac attggtgaaa aagtcggtac tggtcgcggc gacgcggtag atattgctgt
    42781 tgatccgatt gaaggcacgc gcatgacggc gatgggccag gctaacgcgc tggcggtgct
    42841 ggcagtaggc gataaaggct gcttcctcaa tgcgccggat atgtatatgg agaagctgat
    42901 tgtcgggccg ggagccaaag gcaccattga tctgaacctg ccgctggcgg ataacctgcg
    42961 caatgtagca gcggcgctcg gcaaaccgtt gagcgaactg acggtaacga ttctggctaa
    43021 accacgccac gatgccgtta tcgctgaaat gcagcaactc ggcgtacgcg tatttgctat
    43081 tccggacggc gacgttgcgg cctcaattct cacctgtatg ccagacagcg aagttgacgt
    43141 gctgtacggt attggtggcg cgccggaagg cgtggtttct gcggcggtga tccgcgcatt
    43201 agatggcgac atgaacggtc gtctgctggc gcgtcatgac gtcaaaggcg acaacgaaga
    43261 gaatcgtcgc attggcgagc aggagctggc acgctgcaaa gcgatgggca tcgaagccgg
    43321 taaagtattg cgtctgggcg atatggcgcg cagcgataac gtcatcttct ctgccaccgg
    43381 tattaccaaa ggcgatctgc tggaaggtat tagccgcaaa ggcaatatcg cgactaccga
    43441 aacgctgctg atccgcggca agtcacgcac cattcgccgc attcagtcca tccactatct
    43501 ggatcgcaaa gacccggaaa tgcaggtgca cattctctga gtgatttgat cgattgagcc
    43561 ttccagtcct tcgggactgg aatttttttg ttcggagaac gaagataagg caagtcaatc
    43621 aaaacaggag aaaaacatgg ctgattgggt aacaggcaaa gtcactaaag tgcagaactg
    43681 gaccgacgcc ctgtttagtc tcaccgttca cgcccccgtg cttccgttta ccgccgggca
    43741 atttaccaag cttggccttg aaatcgacgg cgaacgcgtc cagcgcgcct actcctatgt
    43801 taactcgccc gataatcccg atctggagtt ttacctggtc accgtccccg atggcaaatt
    43861 aagcccacga ttggcggcac tgaaaccggg cgatgaagtg caggtggtta gcgaagcagc
    43921 tggcttcttt gttctggatg aagtaccgga ctgcgaaacg ctatggatgc tggcaaccgg
    43981 tacagcgatt ggcccttatt tatcgattct gcaactaggc aaagatttag atcgcttcaa
    44041 aaatctggtc ctggttcacg ccgcacgtta tgccgccgac ttaagctatt tgccactgat
    44101 gcaagaactg gaaaaacgct acgaaggcaa actgcgcatt cagacggtgg tcagtcggga
    44161 aacggcagcg gggtcgctca ccggacggat accggcatta attgaaagtg gggaactgga
    44221 aagcgcgatt ggcctgccga tgaataaaga aaccagccat gtgatgctgt gcggcaatcc
    44281 acagatggtg cgcgatacac aacagttgct gaaagagacc cggcagatga cgaaacattt
    44341 acgtcgccga ccgggccata tgacagcgga gcattactgg taagcggtta cttatcgata
    44401 aacggcacga tgagcaaatc cgcactcatc ttattgatca tcccgcgata tgccggcatc
    44461 aaacggttga taaatgagtg atgatgacca cagacaagga ggtcgcactg ctctttttgc
    44521 ataatttcca gcagtgtttc cggcatttct ccgcgttcaa tacgcagttt tgtcttcggc
    44581 cattgaatat ttttcgtcag tttatacagc ttgttatccg acttattctt caacaattga
    44641 agaatatctt ctgttgcagg gaagtagata cccggataca actcgcttaa gccatcatca
    44701 atatgaatta acgtcaggtg agcgtcatta tgtctggcga gctccagggc tttattcacc
    44761 agtaaggcat cttcttcatt cccggaaatt gccacgccaa tgtgtttata agccatgttt
    44821 aactccttct aaagcaactc catcaagcta acgaacgcag tgataactca aaaataatca
    44881 tctcggcctg gcatgagaaa gtgaaggccg catcaagctc aacttgcccc tccagatcct
    44941 taatcttata ggccacgctg gccccttcat ctgcagagga tgcagcggcg gcaagctctt
    45001 tcagtttggt cataaaagac tctgcttctt cgcgggtggc aaaaaagcgc gaaaacttac
    45061 tggtacagtt gtcgttatca atcaccgtac cgatatcaat cgcacatcct ttagtactgc
    45121 atttatctac gacatctttc atagggcacc tctgtatatt cgcctctctg tcgattacga
    45181 cttaagaata atgcgccgtt aagggaagaa caaacaagta atgatcttat ggattaccat
    45241 tttttttgat attgcttaag gtgaagagtt cagaaggatt ttccgggtat tgtcatttgc
    45301 atcgtggaaa taacaatccc ggaaaaaaat agcagtaaga gatatattaa aggttaatta
    45361 cttgatttat tgtcggcttt atacttcaca tcctgagtat ctttaccata tttattttcg
    45421 ccttgtgtgc caacaaacgc accaagatcg ataagcatca tcaccagaat caacgtcggg
    45481 acaaaacgcc ccaccgccca ttgccagaca cccggtaaaa tcgctcagtt acccgccagc
    45541 agcatccacg ccacaatcat cagaaatgcc catgcgccgg aacgcccgcg atcatgcaag
    45601 cgcttaacag ttactgccgc tgttggccag agcaagcaca caaggcaaaa cgccgcggtc
    45661 tgaatatcga gtagattctt acccgccagt gagaaaagca ccagcatgcc tgcgaaccac
    45721 aggcctatcc aaatccagaa atcacggcgt ccaatacgcc ctttaaatga gaataaccat
    45781 tgctgtatgg tcatgtaagt tccttgatgg ttgtcttttc caggattcta cccttttgac
    45841 aagggcgaca gatatcgttt taatcggagc cagtcataac aaaaggtact gtcaatgaag
    45901 ccagggtgta cgctgttttt tctcttatgt tctgcattaa ccgttacaac aacggcgcat
    45961 gcacaaacac cagatacggc aacgaccgcg ccttatctgc tggctggagc ccctactttc
    46021 gatctctcca tcagccagtt tcgagaagac tttaacagcc agaatcccag cctgccactg
    46081 aacgaatttc gtgccatcga cagcagtccc gacaaagcca atctcactcg tgctgcaagt
    46141 aaaattaacg agaacttgta tgcttctaca gcgctggagc gcggtacctt aaaaatcaaa
    46201 agcattcaaa tgacctggct acccatccag gggccagagc aaaaagcagc gaaagcgaaa
    46261 gctcaggaat acatggcagc agtgatccgc acactcaccc cattaatgac caaaacacaa
    46321 agccagaaaa aactgcagtc gctactaacg gcggggaaaa acaaacgtta ttacaccgag
    46381 acagaaggtg cactgcgtta tgttgtcgcg gacaacggcg aaaaggggct gaccttcgct
    46441 gttgaaccga ttaagctggc gctatctgaa tcgcttgaag gtttgaataa atgacaaaaa
    46501 gcaaagcctt tgtgccgatg aatctctata ctgtttcaca gacctgctgc cctgcggggc
    46561 ggccatcttc ctttattcgc ttataagcgt ggagaattaa aatgcgacat cctttagtga
    46621 tgggtaactg gaaactgaac ggcagccgcc acatggttca cgagctggtt tctaacctgc
    46681 gtaaagagct ggcaggtgtt gctggctgtg cggttgcaat cgcaccaccg gaaatgtaca
    46741 tcgatatggc gaagcgcgaa gctgaaggca gccacatcat gctgggtgcg caaaacgtgg
    46801 acctgaacct gtccggcgca ttcaccggtg aaacttctgc cgctatgctg aaagacatcg
    46861 gcgctcagta catcatcatc ggccactctg aacgtcgtac ttaccacaaa gagtctgacg
    46921 aactgattgc gaaaaaattc gcggtgctga aagagcaggg tctgactccg gttctgtgca
    46981 tcggtgaaac cgaagctgaa aacgaagcgg gaaaaactga agaagtttgc gcacgtcaga
    47041 tcgacgcggt actgaaaact cagggtgctg cggcattcga aggtgcggtt atcgcttacg
    47101 aacctgtatg ggcaatcggt actggcaaat ctgcaactcc ggctcaggca caggctgttc
    47161 acaaattcat ccgtgaccac atcgctaaag ttgacgctaa catcgctgaa caagtgatca
    47221 ttcagtacgg cggctctgta aacgcgtcta acgctgcaga actgtttgct cagccagaca
    47281 tcgacggcgc gctggttggc ggtgcttctc tgaaagctga cgctttcgca gtgatcgtta
    47341 aagctgcaga agcggctaaa caggcttaag tctgacaggt gccggatttc atatccggca
    47401 cttactttcc ttaactcctc gccttaacgc aaaatctcac actcatgatc ctggatttcc
    47461 tcggctgaag cacggttcag cgtcagtaga tttcgttgtg tcgccagcaa cacaaacgaa
    47521 ttatcactct gccgtaccat cgccagcccg tagcttccca tatgttcccg cgcctcaggc
    47581 acttcttctg caagcatcat aaatgggctg cgttgtacca gttcgctttc cgttacccga
    47641 cgcgccaggt attcatgccc gcgcaaacca cctggcagtg gcaaccagcg gctgctgatg
    47701 ttcgccagat ttttatccag ttgttcgcgc acatcaggac gaatacaaga gatatgaata
    47761 tgaaaatggt tttgcgtacg cccggtgcgg gagttgatcg ccaaagaaac cgcgcgatcg
    47821 ggaaccggct ggccgtattt tttgctcata aaatcacgcg cctgccaggc caaccaaaag
    47881 aagttcggcg ttgaaggatc ggtcaacaaa ggactttcag taccgttaat acgatacgtt
    47941 ggcatcaaca gatattgcag tggaccatta agatctttta aaaccacgta tccggcattg
    48001 ggtttgactt ccgcacatgg cgaaggattt tgattttgct gctgattggg caaacattcc
    48061 tcaaggacaa tcttacgtaa tgtatccgac tcttcaccgg ttaatttcca gtaaccaata
    48121 ccggcagcca caacggcgat aactatcatc accaaaaaaa gaagacccgc ttttttcatc
    48181 tttttttccc tgtacctcaa agagatgcaa gggtaacgca aaatcgtgac aaataaaaaa
    48241 cccggtcgcg agaaaccacc gggtttgata attatcctag ggaaatcagc gtttgctgat
    48301 ctgatcgaac gtaccgccgt tagcaaaatg ctctttttgc gctttcgtcc agccgccgaa
    48361 ctcttcatca atggtgaata acttcagctt tggaaacgca ttttcgtact ttttcgctac
    48421 ttcagcgtcg cgcggacggt agtagttttt cgcggcaatt tcctgacctt ctggcgagta
    48481 gagatatttc aggtaggctt ccgccacctc tttggtgcct tttttctcga ccactttatc
    48541 gaccaccgac accgttggct ctgcgaggat agactcactc ggcgtgacga tttcgaattt
    48601 atctttcccc agttcattcg ctgccagcag agcttcgttt tcccaggcga tcagtacatc
    48661 gccaatcccg cgctcgacaa aggtgttagt ggagccgcgc gcgccagaat ccagaacttc
    48721 gacgttttta tacagtgccc gaacaaaatc ctgtgctttt gcctgatcgt tgttgttgtg
    48781 atgcagcgcg tagccccagg cagccaggta gttccagcgt gcgccaccag agcttttcgg
    48841 attaggcgtg atcaccgaaa cacccggttt aatcagatcg ttccagtcat ggatctgctt
    48901 cggattgccc ttacgcacca ggaaaacaat ggtggaagtg tacggtgcgg agttatccgg
    48961 cagacgtttg atccactctt tatcaatccg cccgcgttcc gcaatcgcgt ccacgtcata
    49021 ggccagagcc agcgtgacaa catcagcttc aataccgttg attaccgacg tcgcttgttt
    49081 acctgaacca ccgtgtgact gacgaatcac cacgttatca ccagtttgct gtttccagtg
    49141 ggagctgaat gccttgttgt actgttcgta caattcgcgc gttggatcat atgaaacgtt
    49201 aagaagctga atatcctttg ccataacgct ggttgccgcc agcaaaaatg ttaaccctac
    49261 gccccacttg ttcatcgccc gactctctta tgttgtgttg tgatgagcaa agcgtgccag
    49321 aaggttaacc aaacattaaa gaataaaaaa agattggcta taacttgcgg gtatatgttg
    49381 agggattaaa aaggcggaag aaatccgcct catattgctg acaaagtgcg ctttgtccat
    49441 gccggatgcg gcgcgaacgc cttatccggc ctacaaaagt ttgcaaattc aataaattgc
    49501 agaattcatg taggcctgat aagcgaagcg catcaggcat ttttgcttct gtcatcggtt
    49561 tcaggctaaa ggaatctgcc tttttccgaa atcattaata cagttttttc gcgcagtcca
    49621 gccagtcacc tttgaacgga cgcttcatgt tttcgatagc gtcgatgatg tcgtggtgaa
    49681 ccagctgttc gttctggata cctacacaac gaccgccgta acctgccagc agcagatcga
    49741 tagcgtaagc gcccatacgg gaagccagaa tacggtcgta aggcaccgga gaaccaccgc
    49801 gctggatgtg gcccagcaca gttgcgcggg tttcacgacc ggtttctttc tcgatgaaat
    49861 gcgccagttc gtcaacatca cacatatgtt cggtaatcgc cacgatcgcg tgttttttac
    49921 ctttcgcgat acccgctttg atttcgttta ccaggtcttc acggctgaat tcaacttccg
    49981 gaactacaac gaattcacag ccaccggcaa tggccgcagc cagggtcaga tcgccacaat
    50041 aacggcccat cacttccacc acggaaatac gctggtgaga agaagaggtg tcacgcagac
    50101 ggtcgatcgc ttctacaacg gtgctcagcg cagtgaagaa accgatagtg tagtcagtgc
    50161 ctttgatgtc gttgtcgata gtgcccggca gaccgatgca cgggaagccc atttcggtca
    50221 gacgcattgc acccatgtag gaaccgtcac cgccgataac caccagcgcg tcgataccac
    50281 gttttttcag gttttcgata gccacggcgc ggatgttctc gtcgcggaat tccgggaaac
    50341 gcgcagaacc gaggaacgta ccgccacggt tgatcatgtc agacacgctg taacggtcta
    50401 gctgtaccat acggtcttca tacagaccca gatagccgtc ataaataccc attacttcca
    50461 gaccttctgt cagcgcagaa cgaacaaccc cgcgaattgc ggcgttcatg cctggcgcat
    50521 caccgccgct tgtcaacaca cctattttct taatcatgac tacctctgaa ctttggaatg
    50581 caaaatgaaa tctgttgccg gaagtcttct tgcacatcga agtgatccaa cgaatgtgca
    50641 aatagtataa caatcacttc ctgctgaatt gattcaggtc aggccaaatg gcggtatttt
    50701 atacacaaaa tgcgggtctg gctctctttt atactgatta tgaaagcata gaccgtttac
    50761 cctccctggg tacgacggaa caggggtcct gatggataat cacatccgat cccggaaaac
    50821 gccgtaaaat agcctgctct acctgatccg ccaccatatg tgcctgaacc aaaggcagag
    50881 agtcttccat ttccaaatga atctgaataa agcgggtcgg ccctgactgc cgcgtgcgaa
    50941 gatcgtgagc gccgctaaca cccggccagg aagtcacgat atcaataatt tcttgccgtt
    51001 cttcatcagg caatgcgcga tccagtaatg actgtaccgc ctcatatccc atgcgtaacg
    51061 cgctatataa aatatagatg ccgattccca atgcaaacag agcatcggcg cgatgccagc
    51121 cgtaccaaga caaccccagc gccagcagaa ttgcgccgtt catcataaca tcagactggt
    51181 aatgtagcat atcagcccgc accgcctggc tttgcgtccg gcgcaccacc caccgctgaa
    51241 acgagacaag gataatcgta caaattagcg ccacaattgt cacgataacc ccgacgcctg
    51301 gatctgtcat cggtgttgga gataccagat gttgaatacc cgtcaaaaac aggaatagtg
    51361 ccgaaccgga gataaacata ctttgcgcca gcgccgcgag ggactcagct ttaccgtgac
    51421 caaacgagtg attatcgtcg gcaggttgca gggaatatcg caccaccagt aaattcgtca
    51481 acgacgcgcc gatatccacc agcgaatcca ccagcgcggc gagaatactc accgacccgg
    51541 tataccacca tgcaaaaatt ttaatcagca atagcaacga agccatcgcc gtcgcagcaa
    51601 tcgccgcccg actgaccagc cgtccataag attgattcat aaatactccc gctatcaact
    51661 gacgctagta taacggaagc aaatcatctg caatgcatta agcagcaggc aaattgagga
    51721 taaaaaaaac cccacatcat gtgggggaag acagggatgg tgtctatggc aaggaaaaca
    51781 gggtttacta ctgggaacgt gagttgctac tactcaatag cttcaacgat gaactttttt
    51841 gccattgcgt cacgtcacgc aactgctcca ttcgttgttg atgtttctcg tttaaaaccg
    51901 cttgctgctc cggcgttaac aggcgataca tttggttgcg gacttttgcc atctcaacct
    51961 gacgagcaat ttgctcattc gccatttttt ctgcctgtgc gcgcacagcg ttttcatcaa
    52021 aattttctgc ggtgacaagg cgatgcattg tctccagttc gctaacatta acaggaggct
    52081 gttcgtgccg ggcctgttgc ataagatctc gcatctgctg acgctgatgt tcggttaaac
    52141 ttatgccgtc gaacatatgg ctctgcgtac tgcgctgcgt aagttcttca cccggatgcc
    52201 agttatcgcc tgaaccgact tcagcagcgt ggcttaatga actgactgcc agcgttgagg
    52261 ccatgacggc agcggtaact atgcgcatca tttgctccca aaatctttct gtcgcgattc
    52321 aacgatagag agtttacgat tcaggctgca aacatgcgtc agggggtgta aaacaacgta
    52381 aagtcatgga ttagcgacgt ctgatgacgt aatttctgcc tcggaggtat ttaaacaatg
    52441 aataaaatcc tgttagttga tgatgaccga gagctgactt ccctattaaa ggagctgctc
    52501 gagatggaag gcttcaacgt gattgttgcc cacgatgggg aacaggcgct tgatcttctg
    52561 gacgacagca ttgatttact tttgcttgac gtaatgatgc cgaagaaaaa tggtatcgac
    52621 acattaaaag cacttcgcca gacacaccag acgcctgtca ttatgttgac ggcgcgcggc
    52681 agtgaacttg atcgcgttct cggccttgag ctgggcgcag atgactatct cccgaaaccg
    52741 tttaatgatc gtgagctggt ggcacgtatt cgcgcaatcc tgcgccgttc gcactggagc
    52801 gagcaacagc aaaacaacga caacggttca ccgacactgg aagttgatgc cttagtgctg
    52861 aatccaggcc gtcaggaagc cagcttcgac gggcaaacgc tggagttaac cggtactgag
    52921 tttaccctgc tctatttgct ggcacagcat ctgggtcagg tggtttcccg tgaacattta
    52981 agccaggaag tgttgggcaa acgcctgacg cctttcgacc gcgctattga tatgcacatt
    53041 tccaacctgc gtcgtaaact gccggatcgt aaagatggtc acccgtggtt taaaaccttg
    53101 cgtggtcgcg gctatctgat ggtttctgct tcatgatagg cagcttaacc gcgcgcatct
    53161 tcgccatctt ctggctgacg ctggcgctgg tgttgatgtt ggttttgatg ttacccaagc
    53221 tcgattcacg ccagatgacc gagcttctgg atagcgaaca gcgtcagggg ctgatgattg
    53281 agcagcatgt cgaagcggag ctggcgaacg atccgcccaa cgatttaatg tggtggcggc
    53341 gtctgttccg ggcgattgat aagtgggcac cgccaggaca gcgtttgtta ttggtgacca
    53401 ccgaaggccg cgtgatcggc gctgaacgca gcgaaatgca gatcattcgt aactttattg
    53461 gtcaggccga taacgccgat catccgcaga agaaaaagta tggccgcgtg gaactggtcg
    53521 gtccgttctc cgtgcgtgat ggcgaagata attaccaact ttatctgatt cgtccggcca
    53581 gtagttctca atccgatttc attaacttac tgtttgaccg cccgctatta ctgctgattg
    53641 tcaccatgtt ggtcagtacg ccgctgctgt tgtggttggc ctggagtctg gcaaaaccgg
    53701 cgcgtaagct gaaaaacgct gccgatgaag ttgcccaggg aaacttacgc cagcacccgg
    53761 aactggaagc ggggccacag gaattccttg ccgcaggtgc cagttttaac cagatggtca
    53821 ccgcgctgga gcgcatgatg acctctcagc agcgtctgct ttctgatatc tctcacgagc
    53881 tgcgcacccc gctgacgcgt ctgcaactgg gtacggcgtt actgcgccgt cgtagcggtg
    53941 aaagcaagga actcgagcgt attgaaaccg aagcgcaacg tctggacagc atgatcaacg
    54001 atctgttggt gatgtcacgt aatcagcaaa aaaacgcgct ggttagcgaa accatcaaag
    54061 ccaaccagtt gtggagtgaa gtgctggata acgcggcgtt cgaagccgag caaatgggca
    54121 agtcgttgac agttaacttc ccgcctgggc cgtggccgct gtacggcaat ccgaacgccc
    54181 tggaaagtgc gctggaaaac attgttcgta atgctctgcg ttattcccat acgaagattg
    54241 aagtgggctt tgcggtagat aaagacggta tcaccattac ggtggacgac gatggtcctg
    54301 gcgttagccc ggaagatcgc gaacagattt tccgtccgtt ctatcggacc gatgaagcac
    54361 gcgatcgtga atctggcggt acaggattgg ggctggcgat tgttgaaacc gccattcagc
    54421 agcatcgtgg ctgggtgaag gcagaagaca gcccgctggg cggtttacgg ctggtgattt
    54481 ggttgccgct gtataagcgg agttaaactc cgcatttgta ggcaggataa ggcgtttacg
    54541 ccgcatccgg catttgagca ggatgcctga tgcgacgctg atagcgtctt atcaggccta
    54601 cactccgggg gttattttcc ccacaaccgg cgggaaaaat cttcgatctt gccgctcagt
    54661 cggcgctttt gcatcgtccg cgtccagctt ttcgataacc cggcggcgga gagtaaacgg
    54721 tgatactgat cgtcatcaaa cggcatatgc catgcgatgg cggcagcctc ctgcacggtc
    54781 acatcgctga cacgggaaac caactccagc ggcgcgtccg cagacacttt ccctggcgct
    54841 atcacgctat acaaccaccc caccttacct gtgttttgca tcaactgcgc tatatcgctg
    54901 atatcaaaat ggtaattgag tttatagcaa ggcgagcgcg gctggctgac ctgaattaat
    54961 gcctctcccc agcggaaaat atcgcccata tgaacattac tttccgtgag accgtcggtt
    55021 gagagatttt cgccaaacgc aggcgcaaca aacaactccg cctgttccgg aaattcccgc
    55081 acccagtaga gatagtgctc acgaggataa tgacacagcg ctctgtctgg cccgccgtga
    55141 acctttttct ccgcctgctc gtcaccttcc agccccagct ccgtcagcat caactcacca
    55201 tcaacctgga ttttggcaat tgcgctgggt ttgccttcgg gataagcctg aatcttgcct
    55261 gtgtatacat caaccggata tcgcatctgt tccctctcca cgagaaattt ccccattaga
    55321 ccatatttat tttatgtcag ccgtgctgtt ttcgcggtaa gtatccaggc aggaactcaa
    55381 tagcaaaccg gacgaacgtt agttcatccg gctgtgg
//
LOCUS       DAJTQY010000017        53212 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.017, whole genome shotgun sequence.
ACCESSION   DAJTQY010000017 DAJTQY010000000
VERSION     DAJTQY010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 53212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53212
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.017"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(12..323)
                     /locus_tag="OO349_001042"
     CDS             complement(12..323)
                     /locus_tag="OO349_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HCR5866205.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            486..582
                     /gene="ffs"
                     /locus_tag="OO349_001043"
     ncRNA           486..582
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="OO349_001043"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            702..1055
                     /locus_tag="OO349_001044"
     CDS             702..1055
                     /locus_tag="OO349_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HCR5866206.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(1097..2647)
                     /gene="pdeB"
                     /locus_tag="OO349_001045"
     CDS             complement(1097..2647)
                     /gene="pdeB"
                     /locus_tag="OO349_001045"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HCR5866207.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSHVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPELGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FLEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDCLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(2811..3281)
                     /locus_tag="OO349_001046"
     CDS             complement(2811..3281)
                     /locus_tag="OO349_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HCR5866208.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(3397..3948)
                     /gene="maa"
                     /locus_tag="OO349_001047"
     CDS             complement(3397..3948)
                     /gene="maa"
                     /locus_tag="OO349_001047"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HCR5866209.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILANLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKGVPDNVVVGGNPARIIKKL"
     gene            complement(4120..4338)
                     /gene="hha"
                     /locus_tag="OO349_001048"
     CDS             complement(4120..4338)
                     /gene="hha"
                     /locus_tag="OO349_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HCR5866210.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(4364..4738)
                     /gene="tomB"
                     /locus_tag="OO349_001049"
     CDS             complement(4364..4738)
                     /gene="tomB"
                     /locus_tag="OO349_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HCR5866211.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQALQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(5284..8433)
                     /locus_tag="OO349_001050"
     CDS             complement(5284..8433)
                     /locus_tag="OO349_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HCR5866212.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWLNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSTPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRNNEDIEHSHTVDHH"
     gene            complement(8456..9649)
                     /gene="acrA"
                     /locus_tag="OO349_001051"
     CDS             complement(8456..9649)
                     /gene="acrA"
                     /locus_tag="OO349_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HCR5866213.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQGYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            9791..10438
                     /gene="acrR"
                     /locus_tag="OO349_001052"
     CDS             9791..10438
                     /gene="acrR"
                     /locus_tag="OO349_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HCR5866214.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            10566..13928
                     /gene="mscK"
                     /locus_tag="OO349_001053"
     CDS             10566..13928
                     /gene="mscK"
                     /locus_tag="OO349_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HCR5866215.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(14140..14301)
                     /gene="rsmS"
                     /locus_tag="OO349_001054"
     CDS             complement(14140..14301)
                     /gene="rsmS"
                     /locus_tag="OO349_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HCR5866216.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPASTVLRALDIVKRDYEKK
                     LTHDDEAEK"
     gene            complement(14315..14842)
                     /gene="priC"
                     /locus_tag="OO349_001055"
     CDS             complement(14315..14842)
                     /gene="priC"
                     /locus_tag="OO349_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HCR5866217.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQSCLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKISRWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHRELEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            14912..15289
                     /locus_tag="OO349_001056"
     CDS             14912..15289
                     /locus_tag="OO349_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HCR5866218.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            15442..15993
                     /gene="apt"
                     /locus_tag="OO349_001057"
     CDS             15442..15993
                     /gene="apt"
                     /locus_tag="OO349_001057"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HCR5866219.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISENYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            16122..18047
                     /gene="dnaX"
                     /locus_tag="OO349_001058"
     CDS             16122..18047
                     /gene="dnaX"
                     /locus_tag="OO349_001058"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HCR5866220.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEGNGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTLVPPQ
                     SAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERLAS
                     VTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELA
                     VKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGA
                     QQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQT
                     LRRFFDAELDEESIRPI"
     misc_feature    17380..17444
                     /gene="dnaX"
                     /locus_tag="OO349_001058"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            18100..18429
                     /locus_tag="OO349_001059"
     CDS             18100..18429
                     /locus_tag="OO349_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HCR5866221.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            18429..19034
                     /gene="recR"
                     /locus_tag="OO349_001060"
     CDS             18429..19034
                     /gene="recR"
                     /locus_tag="OO349_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HCR5866222.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            19144..21018
                     /gene="htpG"
                     /locus_tag="OO349_001061"
     CDS             19144..21018
                     /gene="htpG"
                     /locus_tag="OO349_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HCR5866223.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWAKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTYTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            21199..21843
                     /gene="adk"
                     /locus_tag="OO349_001062"
     CDS             21199..21843
                     /gene="adk"
                     /locus_tag="OO349_001062"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HCR5866224.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKQVAEVRADLEKILG"
     gene            21975..22937
                     /gene="hemH"
                     /locus_tag="OO349_001063"
     CDS             21975..22937
                     /gene="hemH"
                     /locus_tag="OO349_001063"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HCR5866225.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEDGSPLMVYSRQQQQALAQRLPDTPVALGMSY
                     GSPSLESSVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMVPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(22934..23893)
                     /gene="aes"
                     /locus_tag="OO349_001064"
     CDS             complement(22934..23893)
                     /gene="aes"
                     /locus_tag="OO349_001064"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HCR5866226.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPSWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGINYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            24045..25349
                     /gene="gsk"
                     /locus_tag="OO349_001065"
     CDS             24045..25349
                     /gene="gsk"
                     /locus_tag="OO349_001065"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HCR5866227.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEVLYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVELVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSQEESYWDR"
     gene            complement(25482..27158)
                     /locus_tag="OO349_001066"
     CDS             complement(25482..27158)
                     /locus_tag="OO349_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HCR5866228.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKSIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(27396..28616)
                     /gene="fsr"
                     /locus_tag="OO349_001067"
     CDS             complement(27396..28616)
                     /gene="fsr"
                     /locus_tag="OO349_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HCR5866229.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYARLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            28834..30486
                     /gene="ushA"
                     /locus_tag="OO349_001068"
     CDS             28834..30486
                     /gene="ushA"
                     /locus_tag="OO349_001068"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HCR5866230.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNLEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGKTNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(30523..31002)
                     /gene="ybaK"
                     /locus_tag="OO349_001069"
     CDS             complement(30523..31002)
                     /gene="ybaK"
                     /locus_tag="OO349_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HCR5866231.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(31206..32000)
                     /locus_tag="OO349_001070"
     CDS             complement(31206..32000)
                     /locus_tag="OO349_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HCR5866232.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            32138..32479
                     /locus_tag="OO349_001071"
     CDS             32138..32479
                     /locus_tag="OO349_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCR5866233.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNRVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(32694..35198)
                     /gene="copA"
                     /locus_tag="OO349_001072"
     CDS             complement(32694..35198)
                     /gene="copA"
                     /locus_tag="OO349_001072"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HCR5866234.1"
                     /translation="MSQTIDLNLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFASGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            35460..36392
                     /gene="glsA"
                     /locus_tag="OO349_001073"
     CDS             35460..36392
                     /gene="glsA"
                     /locus_tag="OO349_001073"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HCR5866235.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENAEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            36395..37687
                     /locus_tag="OO349_001074"
     CDS             36395..37687
                     /locus_tag="OO349_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5866236.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWGAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASGINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLDSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGAILLSLIVAMVMKRNKTV"
     gene            37812..38246
                     /gene="cueR"
                     /locus_tag="OO349_001075"
     CDS             37812..38246
                     /gene="cueR"
                     /locus_tag="OO349_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001026748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HCR5866237.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAGGLRRDGSDM"
     gene            complement(38220..38678)
                     /locus_tag="OO349_001076"
     CDS             complement(38220..38678)
                     /locus_tag="OO349_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HCR5866238.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(38675..39592)
                     /locus_tag="OO349_001077"
     CDS             complement(38675..39592)
                     /locus_tag="OO349_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HCR5866239.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            39738..40415
                     /gene="fetA"
                     /locus_tag="OO349_001078"
     CDS             39738..40415
                     /gene="fetA"
                     /locus_tag="OO349_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HCR5866240.1"
                     /translation="MQENSPLLQLQNVGYLAGDTKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRDQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            40402..41181
                     /gene="fetB"
                     /locus_tag="OO349_001079"
     CDS             40402..41181
                     /gene="fetB"
                     /locus_tag="OO349_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HCR5866241.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGAIEFIPMQVIPVAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(41244..42098)
                     /gene="cnoX"
                     /locus_tag="OO349_001080"
     CDS             complement(41244..42098)
                     /gene="cnoX"
                     /locus_tag="OO349_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HCR5866242.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLQDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADKPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(42159..42968)
                     /gene="ybbO"
                     /locus_tag="OO349_001081"
     CDS             complement(42159..42968)
                     /gene="ybbO"
                     /locus_tag="OO349_001081"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCR5866243.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPNDVERMNNMGFTGVLIDLDSPESVNRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLERLLPGRVMDKILQG"
     gene            complement(42958..43580)
                     /gene="tesA"
                     /locus_tag="OO349_001082"
                     /pseudo
     CDS             complement(42958..43580)
                     /gene="tesA"
                     /locus_tag="OO349_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
     gene            43551..44237
                     /locus_tag="OO349_001083"
     CDS             43551..44237
                     /locus_tag="OO349_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5866244.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            44234..46650
                     /locus_tag="OO349_001084"
                     /pseudo
     CDS             44234..46650
                     /locus_tag="OO349_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(47362..48456)
                     /gene="mnmH"
                     /locus_tag="OO349_001085"
     CDS             complement(47362..48456)
                     /gene="mnmH"
                     /locus_tag="OO349_001085"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HCR5866245.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMD
                     NDERASVGTCYKQQGSDAALALGHKLVAGEIRQQRMYAWRAACLQNPQGILCCARGGQ
                     RSHIVQRWLHEAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAVWVKAQ"
     gene            complement(48525..49451)
                     /gene="allS"
                     /locus_tag="OO349_001086"
     CDS             complement(48525..49451)
                     /gene="allS"
                     /locus_tag="OO349_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HCR5866246.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSAGTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSCKAVEDIVTLFTQHRPEISGFLEIFGTPRS"
     gene            49681..50163
                     /gene="allA"
                     /locus_tag="OO349_001087"
     CDS             49681..50163
                     /gene="allA"
                     /locus_tag="OO349_001087"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HCR5866247.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            50241..51056
                     /gene="allR"
                     /locus_tag="OO349_001088"
     CDS             50241..51056
                     /gene="allR"
                     /locus_tag="OO349_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HCR5866248.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGRVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            51146..52927
                     /gene="gcl"
                     /locus_tag="OO349_001089"
     CDS             51146..52927
                     /gene="gcl"
                     /locus_tag="OO349_001089"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HCR5866249.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGVSHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            52940..>53212
                     /locus_tag="OO349_001090"
     CDS             52940..>53212
                     /locus_tag="OO349_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HCR5866250.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAI"
BASE COUNT        12829 a        13694 c        14020 g        12669 t
ORIGIN      
        1 cccggagagg gttagtagtt ccagcgataa cgctgcaagt cgatttgccc gcttcccgat
       61 accatcacac cttctgccag taatgcctgt cgctgacgct gtaaatccgg cccggttagc
      121 gaaattgtgc catggcgatt aaccacccgg tgccagggta aggtgctgcc ttcagggaga
      181 cgctttaaca caccgcccac ctggcgcgcg gcgcggggcg atcccgccag tttcgccaca
      241 tcaccgtaag tggtgacata gccttcggga atagcggcga cgatttgcca gacgcgttgg
      301 ggaaatgaat cttctttttc catcttttct tcctgaggta atttttcagc ataatctgga
      361 aaaacgcccg agtgaagtcg cattgcgcaa gaaaccagca tctggcacgc aatgggttgc
      421 aattagccgg ggcagcagtg ataatgcgcc tgcgcgttgg ttttcaacgc tctcaatggg
      481 ggctctgttg gttctcccgc aatgctactc tgtttaccag gtcaggtccg gaaggaagca
      541 gccaaggcag atgacgcgtg tgccgggatg tagctggcag ggcccccacc catttctgcc
      601 tcccaccgtt tcgtcaaaaa atcccagcat ggctaaactt taaccacaac tgacgtcgca
      661 agaattgtct ggctgcgcag tacgcttcgg aggtatgtct gatgaagtat gttgatggtt
      721 ttgtggttgc cgttcctgcc gataaaaagg atgcctatcg ggaaatggcc gctaaggccg
      781 cgccattgtt taaagagttt ggcgcgcttc gtattgtcga atgctgggcc agcgatgtac
      841 cggatggcaa agtgaccgat tttcgtatgg cggtgaaagc ggaagagaat gaagaggttg
      901 tctttagctg gattgaatac ccttcaaaag aggtccgcga cgcagctaat caaaagatga
      961 tgtcggaccc acggatgaaa gagttcggcg agtccatgcc gtttgacggc aagcgaatga
     1021 tctatggcgg attcgagtca atcatcgacg aataggtagc gtgacgggct gtgcggcgtg
     1081 ccgtcagccc aaaaattcac aaatgttgct cggcccagcg taagaaatct tctttcggta
     1141 atgccttgct gtagagccag ccctgaccgt agtgcacgcc atgctggcgt agccactctt
     1201 cttgtttact ggtttcgatt ccctccgcta ccattttcag tttcagtgtt tttgccattt
     1261 cgatgatatg cggcgtgaca tttttatatt ccagcgcatc aacgaaagat ttatcgatct
     1321 tcagaatgtc gacatccaga tcctgtaaat aacttaaact tgaatacccc gtaccaaaat
     1381 catcaagata aatttcatgg cccgcctccc ggtagcgaga aattatcggg gcgctggttt
     1441 tcggatcggc aaactcgcgt tcagtgagtt caagcgcgat ctgtctggga ttaacctgat
     1501 agtgattgat catttcacgc agcaattgcg ggattttttc cgaggtgagc acggtggatt
     1561 caagattgat cgaaatatgc tgctgtggat gctgacgcaa gcagtcgccc atatcttcaa
     1621 agacgcttct tataatcagt agcgtcaatg gctcagaaag gcccgtttgc tgtgccagcg
     1681 gaataaaact atctggtgac aaccaactac cgtctgtctg cggccagcgc gccagtgcct
     1741 cagcaccgac aattttgcca ttggctaagg agacaatcgg ctgatagtgc acgcaaatat
     1801 cacgattttc gatagcatcc tgcagccgat gatgcggtga ttggatacgg cgcaaaatac
     1861 gcagcacaaa catcgctgcc agcaggccaa tcaccaaccc gagcggtaac caaataaaga
     1921 cttgccgatg ccagcctttc tgcaacattt tcgttgaagc ccaggtgatg atcgaaatat
     1981 tcatctccag gaagggcagt atatcgtaaa tgatcccatt attttcgata tgctcaccgg
     2041 gtgttttttg tagcctggta ataattccct gagcaatttc atcgctgctg gttatgacaa
     2101 cgttatgggc attgccaata atggcggcat caatttgcca tgagctatag ggaatgacat
     2161 caataaagga agcggggtcg atcatgacga cataatgtgc cgttcccatg gcgaccatgt
     2221 aacggataat gcctaaatcg ttatgcgatg ttaaccagac acgataacca tctttcgaaa
     2281 ttttacctag ctcgggaaag gtatcgggcg gactttcatg ctccagagac gaacactggg
     2341 gaacgttgtt atcgatataa accacttcct gaatatagcg ataactgtaa gatacccgac
     2401 gcatttccat gagatgggct tcgctacagg cagcgccttg ccatctttcc agctcctgca
     2461 gcgcatcttt cccttgtgtc gccaccttat tggctcgaat agcgacgtgg gaggaatagg
     2521 tatccagctc ttcaataaac gatgtttcta cctgctgatg ggccagccag atgcttaagc
     2581 cgacaggcag caatactgaa agaatcagta ctcccgaaat aaggccgacc agatgtcgtg
     2641 ttctcaccag catatcctta ttaacacagt gtagggaaag tatatctgat tgtggaaaaa
     2701 ggctggcagg agataaggag gtagagctgg tatttcatga agtgatgggt ggaaacgatc
     2761 attgtcattt gaccaggcgc agagctttac gcctggtatg taaccctaac ttaagatgtt
     2821 gattcggcgc gagcgagggt aaaaatatcg taaaaagaca gttcaccttt acggacgatc
     2881 attttttgca gttgttcctt atgaacatcc gcgacacgcg gagagttcaa gatggcttca
     2941 accagcgcgg caggtacaaa gcgcgtgttg taccattcgc cgttatagca aacgcggaaa
     3001 tccagcacgt cgatatcttc atagcggtag cgtgggtaga catcgaaaaa gaagaaacca
     3061 ttaagcagga taaataatac aaccagtagc cagacagagc ctgacagcgt ttcggactgc
     3121 aacatcaccg ccagggtggc aaaaaaagca acgtacatac cgataaacag ccccggatgt
     3181 ttacggataa aactgatact aaagcggggt ttgttgtcgc gtttttcgcg ggtattcaga
     3241 gactcaatcg tttcggtcag caggcgttgt atttcggtca tcgtttgcct tcgtggtatc
     3301 tgcaactttc cagggaacac cctattttaa cgtgggaata gtagagggga agtaacagat
     3361 ctaccacaat tttgcataac agttgcgaaa aaccgattac aattttttaa ttattctggc
     3421 tggattaccg cccacgacaa cgttgtccgg gacacctttt gtgacaactg cacctgaagc
     3481 taccacgacg ttatcaccaa tggtcacacc agggttaatg accgcgcgtc cgccaatcca
     3541 gacgttatta ccgatggtga cgggtttccc cagttcagca ccgctattac gtgctacagg
     3601 gtcgatggga tgtgttgccg tgtagatatg aacgcctggt gccaacatac agttatcacc
     3661 gatgcgaata gggcagacat caagcatcac gcaatcgaag ttggcgaaaa aattattgcc
     3721 gagaaaaatg ttatagccat aatcacagcg aaacgttggc tcaatataag cctctgtcac
     3781 ctgaccgaat aaattagcga gaatttgctg gcgtaatgtg tgctcttccg ccagggaatg
     3841 attgtatcgg tgaataagct gacgagcgcg caggcgatcg cgagataacg tctcatctgc
     3901 cgagcgatac aactcaccag caatcatctt ttctttttct gtgctcatta ctgacctccg
     3961 gttgctgaaa atgcggcaac acagtaagac gaaaagtcag tgaagagaac gttctcatta
     4021 catctgtgtg ataaagatca catagggtat gtcttcatgg cgaaaaagta taaaattctt
     4081 aataaacagc cggttatagc accgaaagcg aattatttat tagcgaataa atttccatac
     4141 tgaggaaggg atcttgtcgt acagtttatt catggtcaat tcggcgaggc ggtgatctgc
     4201 ggctgagtaa aataccgcca gttcattatc tgataattcg tatttatttt tctcgataac
     4261 acgctccagc gtgtcaattg tctggcaacg acgtaaacgc attaaataat cggtttttgt
     4321 taaaggtttt tcggacatac ttctacccat ggttgtaata attctaacaa gataaactcg
     4381 caggattctc tttcgtcgcg ttgacaaaac aacggaatag tcgattacct gacttccgcc
     4441 atttctgaag agcctgcata ttaataccat aactgctgaa caacataaag gtgtcatcca
     4501 gatattcgtc gatctgctca atgagcttat tgtcttcatt atacttaatt ttgtaattaa
     4561 gtgcgaaggt cgcaatatgc tcaatcagtt cattcaactg gaggttgatc gccgaggttg
     4621 ggtcgttaac ccaaccatga ttgctttctt caaggtttgc aagacagtca tgatacaggg
     4681 tttcacagag aaacttaagc tgtgcgatat catgtctttt gggtgagtat tcatccataa
     4741 cgcgtcccct tcttagtggt tgaactaacg gacacctttc gggatggaaa aaacttactg
     4801 acctggactt gccctctcgt ttgttagctt aactataagc cactctttgc aggttttcat
     4861 cgcattatta cgaaacatta caattaggaa ggaatcggga caggactcac tttttcatac
     4921 tagcactaac ccgcagcagg taaaagcagt ttgctcgaac tgttcataac atttcaggat
     4981 tatcgccaga tgaaattggt gacactaata ccaggattgc tctgaatatg acgtaataac
     5041 cgaggaatga ataaagaatt accgcaaata attaagaata gcctctaaat gattatggat
     5101 tattaaatct attagcgcta cttaatataa attaactaac aatcagtatt ttttatgaat
     5161 tttatacgtg gttaatactg gttttcgtat gagatcctga gttggtggtt caattactcc
     5221 ttaatgttcg taggttatgc ataaaaaagg ccgcttacgc ggccttagtg attacatgtt
     5281 gtatcaatga tgatcgacag tatggctgtg ctcgatatct tcattattgc ggctaaagcg
     5341 gcggcgaacc accacaaaga ataccggaac gaagaagatt gccagtaccg ttgcggtcac
     5401 catcccgccc attacaccgg tacctactgc gttctgcgcg ccggaaccag caccagtact
     5461 gataaccagc ggcataacgc cgaggataaa cgccagcgag gtcatcagga tcggacgtaa
     5521 acgcatccgc accgcatcaa gcgtcgcttc aatcagacct ttaccttctt tatccatcaa
     5581 gtctttggcg aattcgacga taagtatcgc gttcttcgcc gacaacccaa tggttgtgag
     5641 cagtcctact tggaagtaaa cgtcattggt caggccacgg aaggtggcag ccagcaacgc
     5701 accgataacc cccagcggaa cgaccagcat aacggagaac ggagtcgacc agctctcgta
     5761 cagcgccgcc agacacagga atacgacaat caacgaaatc gcgtacagtg aaggtgcctg
     5821 gttgccggag agacgttcct gataggacat ccccgtccag tcatagccaa caccggtagg
     5881 cagtttgctc gccagttgtt ccatcagctc cattgcttca ccggtacttt tacccggtgc
     5941 cgcctggcct aagatttcca tggatggcag gccgttgtaa cgttccagac gcggcgaacc
     6001 gtactcccaa cgagaagagg agaacgccga gaatggcacc atctgaccat cagcagcacg
     6061 aacataccag tcgccgatat catccggcag catacggtat ttcgcttctg acatgacgta
     6121 aactttcttc acacgaccgc ggtcgataaa gtcgttcaca tagctaccgc cccatgcagc
     6181 gcccagagtg gtgttaatgt cgttgataga aacacccagc gcctgcgctt tttcctggtc
     6241 gatatcaatc ttaaactgcg gggtatcttc cagaccgttt ggacgtacgc tggtcagcat
     6301 atcagggtgc ttcgctgctt ctgcaagcag ctggttacgc gcctgagtca gtttttcgtg
     6361 accaaggcca gcctggtcaa tcagctcaaa gtcaaagccg gttgcagtac ccagttccac
     6421 gattgcgggc aggttaaagg cgaaaaccat cgcatctttg atttgcgaga aagcgcgtgt
     6481 tgcacgcatg gtaatcgctt caactttgtt ttcttcgccc ggacgatcgg cccagtcctt
     6541 caaggaaacg aacgcaatac cggtattctg accacgtccc gcaaagccga agccgttaac
     6601 ggcgaacacc gactcaacgt tgttcttttc tttggtcaga tagtaatgcg ttacctcatt
     6661 gagcactttc tgcgtacgtt cctgcgttgc acctgctggc agctgaacca tggtcataaa
     6721 tacgccctgg tcctcatctg gcaagaagga gcttggcaga cgcacgaaca gataggccat
     6781 gccgaccacg atgatcagat acagcactag gtaacgcccc gtactgcgca gaataccgcc
     6841 tacgctgtcg gtgtagtggt gcgtgctctt ctcgaacatg cggttcaacc agccgaagaa
     6901 gcctttttta ccttccccgt gatcgccttt ggcaatcggt ttcagcatgg tggcacaaag
     6961 agccggagtc aggatcaacg ctaccagtac cgacagcgcc attgctgaaa caatggtaat
     7021 agagaactga cgatagatag caccagtaga accgccaaag aaggccatcg gtacgaatac
     7081 cgccgacagt accatcgcga taccgaccag agcgccctga atctgcccca tcgacttacg
     7141 ggtagcttct tttggcggca aaccttcttc cgccataaca cgctccacgt tttctaccac
     7201 aacgatagcg tcatccacca acaagccgat ggcgagcacc atcccgaaca ttgttagcgt
     7261 gtttatcgag aagccaaagg cggcaaggac ggcaaaggtc ccgagcaata ccaccggtac
     7321 ggcaatggtc ggaatcaacg tcgcgcggaa gttctgcagg aacagataca taaccaggaa
     7381 cacgaggatg atcgcttcga ccagcgtttt aaccacttcg tgaatagaga ttttcacgaa
     7441 cggcgtggtg tcgtacgggt aaacaatttt cagacccgac gggaagaacg gttccatctt
     7501 cgccagttca gcacggattg ccgcagcggt atccagcgcg tttgcaccgg tcgccagctt
     7561 gatccccaga ccggaagcag gttggccgtt aaactctgcg atgatgtcgt agttctcacc
     7621 acccagctca atcttcgcca catcacgcag cagtacgcgg gaaccatcct gattcacttt
     7681 cagcaggatt ttgccgaact cttcagtaga ggtcagacgc gtctgagcaa taatagaggc
     7741 gttaagctgt tggcctttca ccggcggcgt accaccgagc tgacccgccg caacctgggc
     7801 gttctgcgct ttgatggcgg taatgacatc aaccggcgtt agctggaatt tgttcagctc
     7861 attcgggttc atccagatac gcatcgcgta ctgtgaaccg aacaactgaa catcacccac
     7921 gcccgacgta cggctgatgg catctttcat attcgccgct acgtagtcag agatatcctc
     7981 ctgcgtcatg gtgccatcgg tgttgataac gccgacaacc atcaggaagc tgctggatga
     8041 tttctcaacg ctcacccctt gctgctgaac ttcttgcggc agcaacggca tcgccagctg
     8101 cagtttgttc tgcacctgaa cctgcgcgat atccgcatca gtaccagact caaaggtcag
     8161 ggtgatctgc acggtacccg tggagtcact gttagaggac atgtacatca ggttatcgat
     8221 accgttcata ttctgttcga taacctgtgt caccgtgtcc tgcactgttt tcgcatcagc
     8281 gccggggtag gaggcggaga tcgttactgc cggcggtgca atcgtaggat attgcgccac
     8341 cggcagtttg aggatcgcca gcccccctgc caacatgatg ataatggcga tcacccacgc
     8401 aaaaatcggg cgatcgataa agaaattagg catgtcttaa cggctcctgt ttaagttaag
     8461 acttggactg ttcaggctga gcaccgcttg cggcttgctg gttattatca gcggtaactt
     8521 cttgtgcttt tacctggaca ccaggacgca ctttctgcag cccacttatt actacgcgat
     8581 cgcctgcttt cagaccttct gtcaccagcc acttatcgcc aatagcctgg cttgcaacga
     8641 tcggacgggt ttccactttg tcatccgcgc caactaccag tacggtggca tcaccacgcg
     8701 gcgtacgggt tacgccctgt tgcgggacta aaatagcgtt tggattaagc ccttcttcca
     8761 gacgtgcgcg cacgaacata cccggcagca gagtgtgatc cgggttcggg aagatagcgc
     8821 gtagggtgat agacccagtg gtctgatcaa cggtaacgtc agagaattcc agcgtaccgt
     8881 cctgcgggaa cttaatgccg tcactggtga tcagcgacac tttggctttg ccgttctctt
     8941 gtttcagcgt gccattcgcc agttcctgtt tcaggcgaag gaagtcgttg ctggactggg
     9001 tcacatcaac gtagatcgga tcaagttgct gcacggttgc cagcgcagtc gcctgaccgt
     9061 tctgtaccaa tgcgccttcc gtcacgttcg acttaccaat gcgaccgcta atcggagagg
     9121 tgactttggt gtaagccaga ttgatccgcg cagtttcaac ggcagctttc gccgcagtta
     9181 ccgcagcatt cgcctgttgc gcatcagcca gagcctgatc gtacccttgc ttactgatgt
     9241 actgagtacc gagcagtttc tgataacgat tcaccgtcaa ttgcgcgata ttggctgcag
     9301 cctgggcttt cgccagatca cctttcgcac tgtcgtatgt cgcctgatag gtcgcaggat
     9361 caatctgata gagagagaca ccagcttcga tgtcgctacc ttctttgaaa ttacgcttca
     9421 ggataatccc gctaacttga ggacgaactt ctgcgatccg gtaggcactg gtgcgacccg
     9481 gaagctcggt tgtgatctgc agaggttcag ttttgactgt tactacgcca acggcgggca
     9541 tctgctggcc accttgttgg gcctgtttgt cgtcacatcc tgttagggct aagctgcctg
     9601 agagcatcag aacgaccgcc agaggcgtaa accctctgtt tttgttcata tgtaaacctc
     9661 gagtgtccga tttcaaattg gtcaatggtc aaaagttaat aaacccattg ctgcgtttat
     9721 attatcgtcg tgctatggta catacattca caaatgtatg taaatctaac gcctgtaaat
     9781 tcacgaacat atggcacgaa aaaccaaaca agaagcgcaa gaaacgcgcc aacacatcct
     9841 cgatgtggct ctacgtcttt tctcacagca gggggtatca tccacctcgc tgggcgagat
     9901 tgcaaaagca gctggcgtta cgcgcggtgc aatctactgg cattttaaag acaagtcgga
     9961 tttgttcagt gagatctggg aactgtcaga atccaatatt ggtgaactag agcttgagta
    10021 tcaggcaaaa ttccctggcg atccactctc agtattaaga gagatattaa ttcatgttct
    10081 tgaatccacg gtgacagaag aacgacgtcg attattgatg gagattatat tccacaaatg
    10141 cgaatttgtc ggagaaatgg ctgttgtgca acaggcacaa cgtaatctct gtctggaaag
    10201 ttatgaccgt atagaacaaa cgttaaaaca ttgtattgaa gcgaaaatgt tgcctgcgga
    10261 tttaatgacg cgtcgggcag caattattat gcgcggctat atttccggcc tgatggaaaa
    10321 ctggctcttt gccccgcaat cttttgatct taaaaaagaa gcccgcgatt acgtcgccat
    10381 cttactggag atgtatctcc tgtgccccac gcttcgtaat cctgccacta acgaataacc
    10441 ctgaatctga ctccaggatt tttcctggac attttcgtcg ttgctattct ggttcactgc
    10501 gtcgtgatat tcttgcggtt tgactttttc aggtcgttct tcaggttcag aaaccttcat
    10561 tcatcatgac tatgttccag tattacaaac gatcacggca ttttgttttt tcagcattta
    10621 ttgcttttgt ttttgtcttg ttatgccaga acacggcgtt tgcgcgggcg tcatcgaatg
    10681 gtgatctgcc gacaaaagcg gacctgcagg cgcaacttga ctcactaaat aaacaaaaag
    10741 atctttctgc tcaggacaaa ctggtgcagc aggatctgac agatacatta gccaccctcg
    10801 ataaaatcga tcgcataaaa gaagagacag ttcagctacg gcaaaaagtc gctgaagcgc
    10861 cggaaaaaat gcgccaggcg accgcggcgt taacagcact tagcgatgtc gataacgacg
    10921 aagaaacgcg caaaattctg agtacgcttt cgttgcgcca gctggaaact cgcgttgccc
    10981 aggcgctgga cgatttgcaa aacgcacaaa acgatctggc gtcttataac agccagctgg
    11041 tttcgttaca gacgcagccc gaacgcgtgc aaaatgcgat gtataacgct tcgcagcagc
    11101 tgcaacaaat tcgcagtcgt ctggatggga ctgatgtcgg cgagactgcc ttacgtccca
    11161 gccagaaagt gttaatgcag gctcagcagg cgttgctgaa tgcggagatt gaccagcagc
    11221 gtaaaagcct ggaagggaac accgtcttgc aggatacctt gcaaaagcaa cgtgattacg
    11281 tgacggcgaa cagcgcccgt ctggagcacc agttgcagct gttgcaagaa gcggtaaaca
    11341 gcaagcgcct gactttaact gaaaaaacgg cgcaggaagc cgtctccccg gatgaagccg
    11401 cgcgtattca ggctaatccg ctggtgaaac aggaactgga aattaaccag cagttaagtc
    11461 agcgtctgat taccgcgact gaaaacggta atcagttgat gcagcaaaac attaaagtca
    11521 aaaactggct ggagcgggcg ctgcaatcgg aacgcaatat taaagagcag attgccgtcc
    11581 tgaagggcag cctgctgttg tctcgtatcc tttaccagca acaacaaacg ctgccctcgg
    11641 cggatgaact ggaaaacatg accaaccgca tcgcggattt gcgtctcgaa cagtttgaag
    11701 ttaaccagca gcgtgatgca ctcttccaga gcgatgcgtt cgtcaacaaa ctggaagaag
    11761 gtcacaccaa cgaagtcaac agcgaagttc acgatgcgtt attgcaagtg gttgatatgc
    11821 gtcgcgaatt gctggatcaa ctcaacaaac agttgggtaa ccagctgatg atggccatta
    11881 acctgcaaat caaccagcag cagttaatga gcgtatcgaa aaacctgaaa tccatcctga
    11941 ctcagcaaat cttttgggtg aacagtaacc gtccaatgga ctgggactgg atcaaagcgt
    12001 tcccgcaaag cctgaaagat gaatttaagt cgatgaaaat cacggtgaac tgggaaaaag
    12061 cctggcctgc cgtttttatc gctttcctcg ccggtttgcc gctgctgttg attgccgggc
    12121 tgatccactg gcgtctgggc tggctgaaag cgtatcaaca gaaactggct tccgctgtgg
    12181 gttccctgcg taacgatagc cagctcaaca caccaaaagc gatccttatc gacctgatcc
    12241 gcgcgctgcc ggtgtgcctg attattctcg cggttggcct gattctgttg accatgcagc
    12301 tcaacatcag cgaactgcta tggtcgttca gtaaaaaact ggcgattttc tggctggtgt
    12361 ttggcctgtg ctggaaggta ctggagaaaa atggcgttgc cgtgcgtcac ttcggcatgc
    12421 cggagcagca gaccagccac tggcgtcggc aaattgtccg catcagtctc gcgttgctgc
    12481 ctatccattt ctggtctgtg gtggcagaac tttccccgct gcatctgatg gatgatgtgc
    12541 tggggcaggc gatgattttc ttcaacctgc tgctgattgc cttcctggta tggccaatgt
    12601 gccgcgaaag ctggcgtgat aaagagtcgc acaccatgcg actagtcacc attaccgtgc
    12661 tgtcgattat cccgattgca ttgatggtgc tgactgccac gggttatttc tacaccacgc
    12721 tgcgcctctc cgggcgttgg attgaaaccg tttatctggt gatcatctgg aacttgctct
    12781 accagacggt attgcgcggc ttaagcgtag cggcgcggcg tatcgcctgg cgtcgtgcgc
    12841 tggcgcgtcg gcagaatctg gtgaaagagg gcgcagaagg tgccgaaccg ccggaagaac
    12901 ccaccattgc actggagcaa gttaaccagc agacgctgcg tattaccatg ttgctgatgt
    12961 ttgcgctgtt cggtgtcatg ttctgggcaa tttggtccga tttgatcacc gtgttcagct
    13021 atctcgacag catcacgctc tggcattaca acggcactga agctggcgct gcggtggtga
    13081 aaaacgtcac catgggcagt ctgttgtttg cgattatcgc ctcaatggtg gcctgggcgt
    13141 tgattcgcaa cctgcctggt ttactggaag tgctggtgct ctcgcgactg aatatgcgcc
    13201 agggcgcgtc gtatgccatt actaccatcc ttaactacat cattattgcc gttggtgcga
    13261 tgacggtgtt cggatcgctg ggcgtctctt gggataaact ccagtggctg gccgcagcat
    13321 tatccgtagg tcttggtttt ggtttacaag aaattttcgg taacttcgtc tccggtttga
    13381 tcattctgtt cgaacgtccg gtgcgtattg gcgatacggt aaccattggt agcttctcgg
    13441 ggacggtaag taagatccgt attcgtgcga caacgattac cgatttcgat cgcaaagaag
    13501 tgatcatccc gaacaaagcg tttgttaccg agcgtctgat caactggtcg ttgactgaca
    13561 ctactacgcg tctggtgatc cgtctcggcg tggcctatgg ctccgatctg gaaaaagtgc
    13621 gtaaagtgtt actgaaggcg gcgactgagc acccaagggt gatgcacgaa ccaatgccgg
    13681 aagtcttctt tacggcattt ggtgccagca cgttggatca tgagctgcgt ctgtatgtgc
    13741 gtgaactgcg tgaccgtagc cgtactgtcg atgagctgaa ccgtactatc gatcagctgt
    13801 gccgtgaaaa cgacatcaac attgccttta accagcttga agtgcatctg cacaacgaga
    13861 agggcgatga ggtgacggaa gtaaaacgcg actacaaagg cgacgatccg acaccagcgg
    13921 ttgggtaata accaaagggg gcgacattta gttgccccga gattactgac aaagtgcgcg
    13981 ttgttcatgc cggatgcggc gtgaccgcct tatccggcct acgaaaccgc aagaattcaa
    14041 tatattgcag gagcggtgta ggcctgataa gcgtagcgca tcaggcagtt ttgcgtttgc
    14101 ccgcaatctt aggggacatt tagcggcccc atttatttct cacttttccg cctcatcatc
    14161 gtgcgttaat ttcttttcat aatcacgctt tacaatatcc agcgcgcgca gcacggtact
    14221 ggcagggatc tgattttcct ccagcagcac aatcaaatcg acagccagtt tgacatcgtc
    14281 aggggcattt tcaagtgaca tactctttcc attgctagcg ggttaagcgc gctagcctgt
    14341 tttcgatttt ttccagcgca tggcggcagc gtgccaggcg cgcttcatag gcttccaatt
    14401 cacgatgcag cgtttgctgt tccacgagat cggtcacccg cgccaggcgg gctctgcgtt
    14461 cggcaaccat ctcacgtagc cgccgctcaa aatcctgatg ctgaatacgt ttacgctgcc
    14521 agcgggaaat tttcggtggc gcactgtccc actcgcgcaa tgaccaggcg gaggcttcac
    14581 gcgcgatggc ttccagttgt gccgccagat gttccgccag ccaggccact tgcggcagct
    14641 gttgctgctc aactgcatga cgaagcgcag ccagattatc gcccgcctcg tcgagacaag
    14701 actgtagtgt tgtcgcacga gtctgaaaaa gatgcctgtc gaaacgagca cttagcgtgg
    14761 cgaactgtga caccggggca caacgctgac gcagcgtagc gagctgtcct tccagttttt
    14821 ccagcagcag ggcggttttc acgataagac ctcaatgaaa atgataattg ttatgctaaa
    14881 gtagccacta tgtaagctga caacactcaa tatgcaacga atcattttaa tcatcattgg
    14941 ctggctggcg gtagtgctgg gtacgctggg cgtggtatta ccggtattac cgacgacgcc
    15001 gtttatcctg ctggcggcct ggtgctttgc ccgttcttcc ccgcgctttc acgcctggtt
    15061 gctgtaccgc tcatggtttg gcagctatct acgtttctgg cagaaacatc atgcgatgcc
    15121 gcgcggcgtc aaaccgcggg cgattttgct tattttgctc acgtttgcca tttctctgtg
    15181 gttcgtccag atgccatggg tgcgcatcat gttgctggta attctcgcct gtttgctttt
    15241 ctatatgtgg cgaattccgg tgattgatga aaagcaagaa aagcactgaa gcacaacaat
    15301 cgcagttgca attattgcgt acagccagta cattctggcg ttttcgagca caggcgcagg
    15361 cggtcaaagg ttaaacaaca gttacttttg atacgtttaa aacgcgccgt gagtaccacc
    15421 gtaacaagca ggcatacact tatgaccgcg actgcacagc agcttgagta tctcaaaaat
    15481 agcatcaaaa gcattcagga ctacccaaaa cccggcattc ttttccgcga tgtcaccagc
    15541 ttactggaag acccgaaagc ttacgctctc agcatcgact tgctggttga gcgttacaaa
    15601 aatgcgggca ttaccaaagt tgtcggcacc gaagcgcgtg gcttcttgtt tggcgctccg
    15661 gtagctctgg gtctgggcgt tggctttgta ccggtccgta aaccgggcaa actgccgcgt
    15721 gaaaccatca gtgaaaatta cgacctggaa tacggcaccg atcagctgga aatccacgtt
    15781 gatgccatca aaccgggcga caaagttctg gtggtggacg atctgctggc aaccggcggc
    15841 actatcgaag cgaccgttaa actgatccgt cgtctgggtg gtgaagtggc tgacgctgcg
    15901 ttcattatca acctgtttga tctcggcggc gaacagcgtc tcgaaaaaca gggcattacc
    15961 agctacagcc ttgtcccgtt cccgggccat taattatcgc cagtcttgtg ctgcccacgc
    16021 tacggacagc acaagatgtg cattcagcct cgccgttctg acggggctgt gttagcatta
    16081 ccccttcgtg aatccacctt ccagcgtttc agagcctgcc aatgagttat caggttttag
    16141 cccgaaaatg gcgcccacaa acctttgctg acgtcgtcgg ccaggaacat gtgctgaccg
    16201 cactggcgaa cggcttgtcg ttagggcgta ttcatcatgc ttatcttttt tccggcaccc
    16261 gtggcgtcgg aaaaacctct atcgcccgac tgctggcgaa ggggctaaac tgcgaaaccg
    16321 gcattaccgc gacgccgtgc ggcgtgtgcg ataactgtcg tgaaatcgag caggggcgct
    16381 ttgtcgatct gattgaaatc gacgccgcct cgcgcaccaa agttgaagat acccgcgacc
    16441 tgctggataa cgtccagtac gctccggcgc gtggtcgttt caaagtttat ctgatcgacg
    16501 aagtgcatat gctgtcgcgc cacagcttta acgcactgtt aaaaaccctt gaagagccgc
    16561 cggagcacgt taagtttctg ctggcgacga ccgatccaca gaaattgccg gtgacgattt
    16621 tgtcacgctg tctgcaattt catctcaagg cgctggatgt cgagcaaatt cgccatcagc
    16681 ttgagcacat cctcaatgaa gaacatatcg ctcacgagcc gcgggcgctg caattgttgg
    16741 cgcgcgccgc tgaaggcagc ctgcgagatg ccttaagtct gaccgaccag gcgattgcca
    16801 gcggtgacgg ccaggtttca acccaggcgg tcagtgcgat gctgggtacg cttgacgacg
    16861 atcaggcgct gtcgctggtt gaagcgatgg tcgagggcaa cggcgagcgc gtaatggcac
    16921 tgattaatga agccgctgcc cgtggtatcg agtgggaagc gttgctggtg gaaatgctcg
    16981 gcttgttgca tcgtattgcg atggtacaac tttcgcctgc tgcacttggc aacgacatgg
    17041 ccgccatcga gctgcggatg cgtgaactgg cgcgcaccat accgccgacg gatattcagc
    17101 tttactatca gacgctgttg attggtcgca aagaattacc gtatgcgccg gaccgccgca
    17161 tgggcgttga gatgacgctg ctgcgcgcgc tggcgttcca tccgcgtatg ccgctgcctg
    17221 agccagaagt gccacgccag tcctttgcac ccgtcgcgcc aacggcagta atgacgccaa
    17281 ccttggtgcc gccgcaatca gcgccgcagc aggcaccgac tgtaccgctc ccggaaacca
    17341 ccagccaggt gctggcggcg cgccagcagt tgcagcgcgt gcagggagca accaaagcaa
    17401 aaaagagtga accggcagcc gctacccgcg cgcggccggt gaataacgct gcgctggaaa
    17461 gactggcttc ggtcaccgat cgcgttcagg cgcgtccggt gccatcggcg ctggaaaaag
    17521 cgccagccaa aaaagaagcg tatcgctgga aggcgaccac tccggtgatg cagcaaaaag
    17581 aagtggtcgc cacgccgaag gcgctgaaaa aagcgctgga acatgaaaaa acgccggaac
    17641 tggcggtgaa gctagcggca gaagccattg agcgcgaccc gtgggcggca caggtgagcc
    17701 aactttcgct accaaaactg gtcgaacagg tggcgttaaa tgcctggaaa gaggagagcg
    17761 acaacgcagt atgtctgcat ttgcgctcct ctcagcggca tttgaacaac cgcggtgcac
    17821 agcaaaaact ggctgaagcg ttgagcatgt taaaaggttc aacggttgaa ctgactatcg
    17881 ttgaagatga taatcccgcg gtgcgtacgc cgctggagtg gcgtcaggcg atatacgaag
    17941 aaaaacttgc gcaggcgcgc gagtccatta ttgcggataa taatattcag accctgcgtc
    18001 ggtttttcga tgcggagctg gatgaagaaa gtatccgccc catttgatcg taagcacagc
    18061 ttacgttcgt catccttaac gtgattgaga gagaaaccta tgtttggtaa aggcggtctg
    18121 ggtaacctga tgaagcaagc ccagcagatg caagaaaaaa tgcagaaaat gcaggaagag
    18181 atcgcgcagc tggaagttac cggcgaatct ggcgcaggtc tggtaaaagt gaccatcaac
    18241 ggtgcacaca actgccgtcg cgtagagatc gacccgagcc tgctggaaga cgacaaagag
    18301 atgctggaag acctggtggc tgcagcattc aacgacgcag cacgtcgtat tgaagaaacg
    18361 cagaaagaaa aaatggcctc tgtatcctcc ggaatgcagc tgccgcctgg ctttaagatg
    18421 ccgttctgat gcaaaccagc ccgctgttaa cacagcttat ggaagcactg cgctgtctgc
    18481 cgggcgttgg cccgaagtcg gcgcagcgta tggcgttcac gctgcttcag cgcgatcgta
    18541 gcggcgggat gcgtctggcg caggcgctca cccgggcgat gtcggaaatc ggccactgcg
    18601 ccgattgccg cactttcacc gaacaggaag tctgtaacat ctgttcgaat ccgcgtcgtc
    18661 aggaaaacgg tcaaatctgt gtggtggaga gtccggcgga catctacgcc attgagcaga
    18721 cggggcagtt ttctggtcgt tattttgtgt tgatggggca tctgtcaccg ctggacggca
    18781 tcggtccgga tgatatcggg cttgatcgtc tggaacagcg tctggcagag gaaaaaatca
    18841 ctgaagtgat cctcgccacc aatcccacgg ttgaaggtga agctaccgct aactacattg
    18901 ccgagctttg cgcgcaatat gacgtggaag ccagccgaat cgctcatggc gtaccggttg
    18961 gcggcgaact ggaaatggtc gatggcacca cgctgtcaca ctccctcgcc gggcgtcata
    19021 agattcgttt ttaagcaaac gagagcagga gcacctgctc tcgcttgaaa ttattctccc
    19081 ttgtccccat ctctccctca tcctgttttt aaccttaaaa tggcattatt gaggtagacc
    19141 tacatgaaag gacaagaaac tcgtggtttt cagtcagaag tgaaacagct tctgcacctg
    19201 atgatccatt ctctctattc caataaagaa atcttcctgc gtgagcttat ctctaacgcc
    19261 tccgatgcgg cggacaagct gcgtttccgt gcgctatcta acccggacct gtacgaaggt
    19321 gatggcgaac tgcgcgttcg tgtctctttc gataaagaca agcgcacgct gaccatctcc
    19381 gataacggcg tggggatgac ccgcgacgaa gtgattgacc atctggggac tatcgctaaa
    19441 tccggtacca aatcattcct cgaatccctg ggttctgacc aagcgaaaga cagccagctg
    19501 atcggtcagt ttggtgttgg tttctactct gcgtttatcg tggccgacaa agtgaccgtg
    19561 cgtactcgcg cggcaggcga aaaaccagaa aacggcgtct tctgggaatc ggctggcgaa
    19621 ggtgaatata ccgttgccga tatcaccaaa gaagatcgtg gtactgaaat caccctgcat
    19681 ctgcgtgaag gcgaagacga gttcctcgat gactggcgcg tgcgttccat catcagcaaa
    19741 tactccgacc atatcgcgct gccggtagag atcgaaaaac gcgaagagaa agatggcgaa
    19801 accgttatct cctgggcaaa aatcaacaaa gcgcaggcgc tgtggactcg taacaagtcg
    19861 gaaatcaccg atgaagagta taaagagttc tacaaacaca tcgcccacga ctttaacgat
    19921 ccgctgacct ggagccacaa ccgcgttgaa ggtaagcagg agtacaccag cctgctgtac
    19981 atcccgtccc aggctccgtg ggatatgtgg aaccgtgatc ataaacacgg cctgaaactg
    20041 tacgttcaac gcgtgtttat catggacgac gctgaacagt tcatgccgaa ctatctgcgc
    20101 ttcgtgcgtg gtctgattga ctccagcgat ctgccgctga acgtttcccg tgaaatcctc
    20161 caggacagca cggtaacgcg taacctgcgc aatgcgctga ccaagcgtgt gctgcaaatg
    20221 ctagaaaaac tggcgaaaga cgacgcggaa aaataccaga ccttctggca acagtttggc
    20281 ctggtactga aagaaggtcc ggcggaagat ttcgctaacc aggaagcgat cgccaaactg
    20341 ctgcgttttg cttctactta taccgattct tctgcgcaga ccgtgtctct ggaagactac
    20401 gtttcccgca tgaaagaagg gcaggagaaa atctactaca tcactgcaga cagctatgcg
    20461 gcagcgaaga gcagcccgca cctggaactg ctgcgtaaga aaggcatcga agttctgctg
    20521 ctttccgacc gcatcgatga gtggatgatg aactatctga ctgagttcga cggtaaaccg
    20581 ttccagtcgg tgtctaaagt tgacgagtcg ctggaaaaac tggctgacga agttgatgag
    20641 agcgcgaaag aagcggagaa agcactgact ccgttcatcg accgcgtgaa agctctgctc
    20701 ggcgagcgcg tgaaagatgt ccgtctgact caccgtctga ccgatacgcc agcgatcgtt
    20761 tcgaccgacg cggacgaaat gagcactcag atggcgaaac tgttcgctgc ggcgggccag
    20821 aaagtgccag aagtgaaata catcttcgaa ctgaacccgg atcacgtact ggtgaaacgt
    20881 gcggcagata ctgaagatga agccaagttc agcgagtggg tagaactgct gctggatcag
    20941 gcgctgctgg cagaacgcgg cacgctggaa gatccgaacc tgtttattcg tcgtatgaat
    21001 cagctgctgg tttcctgatg tcatgccgga tgacctttgt gtcatccggc atttttcttt
    21061 tcatcatctg tactttccgc aaattatctc gccattaacc gtttcagcct caggtgcctt
    21121 tcttgaggca atcgcctgtt ggtggtatcg tttatcgctt tttcaaaaaa atcgacacat
    21181 tttaagggga ttttcgcaat gcgtatcatt ctgcttggcg ctccgggcgc ggggaaaggg
    21241 actcaggctc agttcatcat ggagaaatat ggtattccgc aaatctccac tggcgatatg
    21301 ctgcgtgctg cggtcaaatc tggctccgag ctgggtaaac aagcaaaaga cattatggat
    21361 gctggcaaac tggtcaccga cgaactggtg atcgcgctgg ttaaagagcg cattgctcag
    21421 gaagactgcc gtaatggttt cctgttggac ggcttcccgc gtaccattcc gcaggcagac
    21481 gcgatgaaag aagcgggcat caatgttgat tacgttctgg aattcgacgt accggacgaa
    21541 ctgattgttg atcgtatcgt aggccgccgc gttcatgcgc cgtctggtcg tgtttatcac
    21601 gttaaattca atccgccgaa agtagaaggc aaagacgacg ttaccggtga agaactgact
    21661 acccgtaaag acgatcagga agaaaccgtg cgtaaacgtc tggttgaata ccatcagatg
    21721 actgcaccgc tgatcggcta ctactccaaa gaagcggaag cgggtaacac caaatacgcg
    21781 aaagttgacg gtaccaagca ggttgctgaa gttcgcgctg atctggaaaa aatcctcggc
    21841 taatcaagcc gcagcagatc tgctttcggg caggtctgct tatccacctc aggcaatacc
    21901 tctcacagca attagttctt cttcctcact tttccgctac aattatcaac aagttgaatc
    21961 gataagaggc ggtaatgcgt cagactaaaa ccggtatcct gctggcaaac ctgggtacgc
    22021 ccgatgcccc cacacctgaa gcggtaaaac gctatctgaa acaattttta agcgacagac
    22081 gcgtggttga tacctcacgg ttgttatggt ggccgttgct gcgcggcgtg attttgccgc
    22141 tgcgctcgcc gcgtgtggcg aagctgtacg cctctgtctg gatggaagac ggctcgccgc
    22201 tgatggttta cagccgccag caacagcagg cgttggcaca acgtttaccg gatacgccag
    22261 tggcgctggg catgagctac ggctcgccat cactggaaag ctccgtcgat gaactcctgg
    22321 cagagcatgt agatcatatt gtggtgctgc cgctttatcc gcaattctcc tgttctacgg
    22381 tcggtgcggt atgggatgaa ctggcacgca ttctggcgcg caaacgtagc attccgggga
    22441 tatcgtttat tcgtgattac gccgataacc acgattacat taatgcactg gcgaacagcg
    22501 tacgcgcttc ttttgccaaa catggcgaac cggatctgct gctgctctct tatcatggca
    22561 ttccccagcg ttatgcagat gaaggcgatg attacccgca acgttgccgc acaacgactc
    22621 gcgaactggc ttccgcactg gggatggtac cggaaaaagt gatgatgacc tttcagtcgc
    22681 gctttggccg ggaaccctgg ctgatgcctt ataccgacga aacgctgaaa atgctcggag
    22741 aaaaaggcgt aggtcatata caggtgatgt gcccgggctt tgctgcggat tgtctggaga
    22801 cgctggaaga gattgccgag caaaaccgtg aggtcttcct cggtgcgggc gggaaaaaat
    22861 atgaatatat tccagcgctt aatgccacgc cggaacatat cgaaatgatg gctaatcttg
    22921 ttgccgcgta tcgctaaagc tgagcggtaa agaactgagc gccgtcgcga agagcctcgt
    22981 cggcggtttt catcatccgt gaataatgca aaaaggcatg cagcgtacct gggtagagtt
    23041 tgaactcaca gggctgctga tgcgccgcta acgtctggta aagcagacgg ctgtcatcca
    23101 gcagcggatc gaactccgcc ccggcaataa aacagggcgg aacttcgcga gtgagatcat
    23161 tattaaacag acagtaatac ggtgactcgc ggtccgcgtc gttgcttaaa tacgcctctt
    23221 catacatctg caaatcctgt tgcgttaagc catcccagac accgcccaac agacgacgag
    23281 tcacggaatc ccgtaatccg taaagcccat accacagcaa aacacccgca attttaccgc
    23341 aatcgatctg tttatcacgc aaccacaacg cactggcgag cgccagcatg gcacctgcgg
    23401 aatcaccggc aaagccaatg cgggacatat tgatttgata atcctccgcc tgctggtgga
    23461 aataacaaca agcagccaca atttcctcta tcgcttgtgg aaaacgcgct tcaggtgaaa
    23521 gggtgtaatt aataccaatc accgtacatt ggctgtagct tgccagcagg cgcatgatgc
    23581 gatcgtgggt atcgagattg ccgagaataa aaccgcctcc atgcaaataa aatagcgtcg
    23641 ccgggctatc tggctgcgga caaaagagac gtgtttccac ctgcccatat tttgttggaa
    23701 ccatgtaagc tctggttgcc atttctggag cgcccgcatt ccagaatcgg cgctcaagcg
    23761 tgtaatactg tcgttgctca gcaatcgttc ccgttgcggg ccaggacggt aaatccggct
    23821 gaagagtatt cacaacggtc ttcatttcag cagaaataag gtccagaaca ggtagtttgt
    23881 tttccggctt catacaaaaa ctcctttcaa attacgtcat tgtaaggaac cactgccatg
    23941 aaaatgcgat cccgcctgct gatattgaaa ctggctgcgt ctcgcgcgct cccgtcagat
    24001 tgtgttaaca ttcgccgctc agttaaccac ccgtaaaaac aaccatgaaa tttcccggta
    24061 aacgtaaatc caaacattac ttccctgtaa atgcacgcga tccgctgctt cagcaattcc
    24121 agccagaaaa cgaaaccagc gccgcctggg tcgtaggtat tgaccagacg ctggtcgata
    24181 ttgaagcgaa agtggatgat gaattcattg agcgttatgg attaagcgcc gggcattcac
    24241 tggtgattga ggatgacgta gccgaagtgc tttatcagga gctaaaacag aaaaacctga
    24301 ttacccatca gtttgcgggt ggcaccattg gtaacaccat gcacaactac tcggtgctcg
    24361 cggacgaccg ttcggtgctg ctgggcgtca tgtgcagcaa tattgaaatt ggcagttatg
    24421 cctatcgtta cctgtgtaac acttccagcc gtaccgatct taactatcta caaggcgtgg
    24481 atggtccgat tggtcgttgc tttacgctga ttggcgagtc cggggaacgt acctttgcta
    24541 tcagtccagg ccacatgaac cagctgcggg ctgaaagcat tccggaagat gtgattgccg
    24601 gagcctcggc actggttctc acctcatatc tggtgcgttg caagccgggt gaacccatgc
    24661 cggaagcaac catgaaagcc attgagtacg cgaagaaata taacgtaccg gtggtgctga
    24721 cgctgggcac caagtttgtc attgccgaga atccgcagtg gtggcagcaa ttcctcaaag
    24781 accacgtttc tatccttgcg atgaacgaag atgaagccga agcgttgacc ggagaaagcg
    24841 atccgttgtt ggcatctgac aaggcgctgg actgggtaga gctggtgctg tgcaccgccg
    24901 ggccaatcgg cttgtatatg gcgggcttta ccgaagacga agcgaaacgt aaaacccagc
    24961 atccgctgct gccgggcgct atagcggaat tcaaccagta tgagtttagc cgcgccatgc
    25021 gccacaagga ttgccagaat ccgctgcgtg tctattcgca cattgcgccg tacatgggcg
    25081 ggccggaaaa aatcatgaac actaatggag cgggggatgg cgcattggca gcgttgctgc
    25141 atgacattac cgccaacagc taccatcgta gcaacgtacc aaactccagc aaacataaat
    25201 tcacctggtt aacttattca tcgttagcgc aggtgtgtaa atatgctaac cgtgtgagct
    25261 atcaggtact gaaccagcat tcacctcgtt taacgcgcgg cttgccggag cgtgaagaca
    25321 gccaggaaga gtcttactgg gatcgttaag ttatcgtcgg ttcgtaggcc agataaggcg
    25381 ttcacgccgc atctggcatt tggctctcga tgcctgatgc gacgctggcg cgtcttatca
    25441 tgcctacata tttttcatat tttacatccg gcaaccaccg tttaccccgt caccacttca
    25501 cccgccggtg gcgtttccag cagttccagc atagtacggg caatttcacg ttcacccatc
    25561 actacctgat tcgcgccacg ttcggtgata tacgtcactt catcgtcata atgggcgcgg
    25621 gcgataatct caatatccgg gtttttcgcg cgggcagacg ccacaatctc acccgcttca
    25681 taaccgttgg ggatcgtcag gatcagccat tttgcacatt ccagatgtgc cagttgcata
    25741 atttcttcgt tcgctgcatt gcccaataca gcgcggaccc cacgctctcg cagctcatca
    25801 acacgggttc gtgacgtctc aatcaccacc agcggaatat cagaggcgag caatttctcc
    25861 cccagcaggc tgcctacacg accgtaaccc accaatagcg catggttgca aatatccact
    25921 gggatctgtt tctcttcttc gattgcctct tccagcgtct gctcttccag cgtttcggtc
    25981 ttcgccagat atttctccag tagtgcgaac agtaccgggt tgagcataat cgacaggatc
    26041 gcccctgcca gtaccaggtt ttgtccggcc tgcggcagta aattcaatgc cattcccagt
    26101 cccgccagga taaacgcgaa ctcaccaatc tgcgccaggc tggcggcgat ggttaatgcc
    26161 gtacgttggg agtgaccaaa cagacggaca aggaacaggg ccgcgaccga tttaccaaac
    26221 agaataattg ccagcgtcgc cagcactgcc agcggttgct gaatcagaat taacggatca
    26281 aacaacatcc cgactgagac aaaaaacagc accgcaaacg cgtcgcgcaa tggcaacgta
    26341 tcgtgggcgg cacggtgact caattcagac tcgttcagta ccatcccggc aaagaacgca
    26401 ccgagtgcaa aggagacatc aaacagctct accgcaccaa aggcaatccc taacgccagc
    26461 gccagcactg acagggtaaa cagctcgcga gaaccggttg ccgcgctgcg tgccataatc
    26521 cacggcacca gacggcgacc taccagcatc ataatggcga taaatgcgat caccttgccg
    26581 atggtgatcc ccatatcgac tgcaagagtg gcaaagccca catcgccctg ttccatcatt
    26641 cctgccactg cgggcagcaa caccagcgtc agaaccatca ccaggtcttc cacaatcaac
    26701 caaccgatgg cgatttgccc acgctgactg tctattaatt gccgttcttc aagtgcgcgc
    26761 agtaacacca cggtactggc ggtggaaaga cataagccga acacgatacc ggtcattaac
    26821 gaccagccca gcacggcgga gagcgccata cccagcagcg tcgccacggc tatctgggcg
    26881 atcgcgccgg gaatggcgat ggactttacc gccatcaaat ccttgagcga aaaatgcaaa
    26941 ccgacgccga acatcagcaa aataacgccc agttcagcca gttccggcgc aagcttggta
    27001 tcggcaacaa agcccggagt gaatggtcct gccagcacac ccgctaacag atatcccacc
    27061 agaggagaaa tacgtagttt attggccagc atgccgagga taaaggcgag cacaaggccg
    27121 ccaacaatgg tggtgataag cggggtggcg tgatgcattc cgtctccttt tcctggtggt
    27181 tattgtccat ttttggctgg gaaaaccaaa attacaggta atagtttatg acaatttcat
    27241 tgatgatgtt tatgaataat tgttgaattt tgcagaaaaa tggaattagc tgcaaaaaaa
    27301 gcacggttcg gaaaacagaa ggcggtttaa cagaggaaag gcttatggcg actgtaaaac
    27361 gatgcagcca aagtttgcct ttggctgcaa tgaaatcagt ctttatgccg gttatcaggc
    27421 aggaatatgg tcaacatccc caatagtggc aggaaagcac agattttata gactaactcg
    27481 atgctggtgt gatcggcgat aagccccaga actgccgctc ccagacctcc catgccgaaa
    27541 gcaaaaccga aaaagagtcc agaaaccata ccgatacgtc ccggaagtag ctcctgagcg
    27601 tagaccagaa tggcagagaa tgccgaagcg aggataaatc caataatcac cgttaaaacc
    27661 cccgtccagt gcaggcgggc gtagggtaaa atcagcgtaa acggcgcaac gccgaggata
    27721 gagccccaaa tcacatattt ccgtccaatt ttatccccta caggcccgcc gatcaccgta
    27781 cctgccgcaa cggcaaacag gaaggcaaac agatgaagct gggcattctg gatagataat
    27841 ccgaattttt gcatcagata aaaggtgtaa tagctgctga tgctcgccat atagaaatat
    27901 ttcgagaaaa tgaggattaa cagaatgctg actgccagta ccactttgtt gcgcggcagt
    27961 ggattgatta tcgtcgcttt gggttttcct ttattcattc ggtgctgtgc cgagtaccaa
    28021 cggctaattt gcgccaacac cacgatcgcc agcagtgccg caagcacaaa ccaggcaacg
    28081 ttgcctttgc cataaggcgc gataatcacc gccgccagca agggtcccag ggaactgcca
    28141 aagttgccgc cgacctgaaa gatagattgc gccaggccat gccgcccgcc ggaagccata
    28201 cgggctacgc gagaagattc cggatgaaag accgatgaac cggtaccgac cagcgccgct
    28261 gccagcagaa ctgcgccaaa actgcccgcc agcgcaagca gcaccagacc acttaaggta
    28321 aagcacatgc caattggcaa cgaccacggc atcggatatt tatcggtcca gtagccgacc
    28381 actggttgca gtagcgaaga ggcgagctgg aaggtgaggg ttatcatgcc aatctgcata
    28441 aatgtcagag aaaattctga ctgaagcagc ggataaatcg ccagaatcag cgattggatc
    28501 atgtcgttca gcagatgtga gaggctgata gcacctaaaa taccaaacga tgttcgggcc
    28561 ttggtcgttg acgcagccgc gcccgccaca ggctgggttt gttcactcat tgccatagga
    28621 aagtcacttt ttcagggttg cgatgtaaag aatgatctta tttgtgatta ttacccgact
    28681 aacatacctg tatgcgtcgt ctgaaggaag tctcaacgcc gaatacagaa tttctaatct
    28741 ggatgcagat ttatcttcac cggacgcaga cttgtctatg atgtcgcgtc atactatttt
    28801 tcaacacgtt gaaatcaggt cagggagaga agtatgaaat tattgcagcg gggcgtggcg
    28861 ttagcgctgt taaccacatt tacactggcg agtgaaactg ctctggcgta tgagcaggat
    28921 aaaacctaca aaattacagt tctgcatacc aatgatcatc atgggcattt ttggcgcaat
    28981 gaatatggcg aatatggtct ggcggcgcaa aaaacgctgg tggatggtat ccgcaaagag
    29041 gttgcggctg aaggcggtag cgtgctgcta ctttccggtg gcgacattaa cactggcgtg
    29101 cccgagtctg acttacagga tgccgaacct gatttccgcg gtatgaatct ggtgggctat
    29161 gacgcgatgg cgatcggtaa tcatgaattt gataatccgc tcaccgtatt acgccagcag
    29221 gaaaagtggg ccaagttccc gttgctttcc gcgaatatct accagaaaag tactggcgag
    29281 cgcctgttta aaccgtgggc gctgtttaag cgtcaggatc tgaaaattgc cgttattggg
    29341 ctgacaaccg atgacacagc aaaaattggt aacctggaat acttcactga tatcgaattt
    29401 cgtaagcccg ccgatgaagc gaagctggtg attcaggagc tgcaacagac agaaaagcca
    29461 gacattatta tcgcggcgac ccatatgggg cattacgata atggtgagca cggctctaac
    29521 gcaccgggcg atgtggagat ggcacgcgcg ctgcctgccg gatcgctggc gatgatcgtc
    29581 ggtggtcact cgcaagatcc ggtctgcatg gcggcagaaa acaaaaaaca ggtcgattac
    29641 gtgccgggta cgccatgcaa accagatcaa caaaacggca tctggattgt gcaggcgcat
    29701 gagtggggca aatacgtggg acgggctgat tttgagtttc gtaatggcga aatgaaaatg
    29761 gttaactacc agctgattcc ggtgaacctg aagaagaaag tgacctggga agacgggaaa
    29821 agcgagcgcg tgctttacac tcctgaaatc gctgaaaacc agcaaatgat ctcgctgtta
    29881 tcaccgttcc agaacaaagg caaagcgcag ctggaagtga aaataggcaa aaccaatggt
    29941 cgtctggaag gcgatcgtga caaagtgcgt tttgtacaga ccaatatggg gcggttgatt
    30001 ctggcagccc aaatggatcg cactggtgcc gactttgcgg tgatgagcgg aggcggaatt
    30061 cgtgattcta tcgaagcagg cgatatcagc tataaaaacg tgctgaaagt gcagccattc
    30121 ggcaatgtgg tggtgtatgc cgacatgacc ggtaaagagg tgattgatta cctgaccgcc
    30181 gtcgcgcaga tgaagccaga ttcaggtgcc tacccgcaat ttgccaacgt tagctttgtg
    30241 gcgaaagacg gcaaactgaa cgaccttaaa atcaaaggcg aaccggtcga tccggcgaaa
    30301 acttaccgta tggcgacatt aaacttcaat gccaccggcg gtgatggata tccgcgcctt
    30361 gataacaaac cgggctatgt gaataccggc tttattgatg ccgaagtgct gaaagcgtat
    30421 atccagaaaa gctcgccgct ggatgtgagt gtttatgaac cgaaaggtga ggtgagctgg
    30481 cagtaatccg aaagtgccgg atgtttgcat ccggcacaat gcttaatcgc ggcgggcgat
    30541 atcagcaaat ttggcatcga ggatctttgc cagatcgcct gccgccagtt cgatatccag
    30601 tccgcgcttg ccgccggaaa cataaatagt ggcaaattct tgtgcggggg cgtcgataat
    30661 cgttggcaga cgttttttct gccccagtgg gctaatcccc ccaaccaggt atcccgtcga
    30721 acgctgcgcg accatcggat cggccatctc aactttcttg gcacccagcg cttttgctac
    30781 ttttttaaga tccagttgac ctgcgaccgg cgtaacggcc acggcaaggt gtttcatatc
    30841 accgttcact gccaccagca gcgttttgta gacctgatcc ggattcaaac ctaatttttt
    30901 gacgacttca tcgccaaaat tggtttcagc cggatcgtgc tcgtaggtat ggatttgaaa
    30961 cgaaatcttg tttttttcga gtaatttaac tgcgggtgtc atggtaattt tcctgcctta
    31021 aacaaaaaaa cgaagcaagc atacgcctga tggcggtcca aaaaaaagag tcatcttgcc
    31081 taagagtatt ggcaggatgg tgagattgag cgacaatcga gttacaccgt cgcttaaagt
    31141 gacggcataa taataaaaaa atgaaattcc tctttgacgg gccaatagcg atattggcca
    31201 tttttttagc gcaacatttg cggcaaattc ccttctccat acaggtgtag tgcaccgacc
    31261 gcgaccacat atcgccccgg cggcatggcg cgtaatttat cccgccaggc gagatttcgc
    31321 tgatgcatca gcacatcgta cagcgactga ctgaacgtat tgggcagcgt tatatcatta
    31381 ttttgcggcg gtgcattcag ccaccagctc atcatttgtt gcagcaaccg tgcgttggta
    31441 tgccagtggg tcagcgtatc gtccagcagc gccagtcctt tgtcagggag ctggagcaac
    31501 atggcaatct ggttttcagc cccttccagt tcaatcacgg gtttatgttg ttgcttcgcc
    31561 gcctgcaata gctggtaatc gataccgtat tctgcccgca gccccagttt ttgtgcctgc
    31621 gtcgcctgaa gaaccatcgc gatttgccac agcggttggg tagaaaagag tgagggagaa
    31681 atgcccatct cctggctaat gtgctgcagg ttttggagtt gctcctcgct aatgcgctct
    31741 tccagcgcct cgcaggcagg caaattagca aaaggcgtat cgctggtgga aacatccgcc
    31801 tcgacgatca gcgcatcggc gtttttgagc tttttgagca aacgggtggg caggggagcc
    31861 atatcgtggc tacccatatg aatactgcca atcagatgaa aatggcgatt gccggggagg
    31921 gtgatatcga tggcaggcca ggtgtaatga ttaccgcgca gcgcggccca aagcgttttt
    31981 acccggtaca acagatccat acgacctccc tttgtgaaat atcatgctag cgcgcggtga
    32041 gggatggcgc aaatggagtt ggggacttgt ttaatgtgtt tgtatgattc agtatgttct
    32101 tgcatcgcta ttcacaggga agcaacagtt aaaaaccatg aaacaggcaa caagaaaacc
    32161 gacgacaccc ggagatattc ttctctatga atatctggaa ccgctcgatt tgaaaatcaa
    32221 tgagttagca gagttgctgc atgttcatcg taatcgcgtc agtgcactga tcaataacaa
    32281 tcgtaaactc accactgaga tggcattccg tctggcgaaa gtttttgata ccacagtcga
    32341 ttttaggcta aacctccagg cggcagttga tctttgggaa gttgaaaaca acatgcgcac
    32401 ccaggaagaa ttgggacgga ttgaaacagt ggctgaatat ttggcacgcc gtgaagagcg
    32461 tgcaaaaaag gtcgcgtaag gccaaaacat cgcttcatct cgtaggccgg ataaggcgtt
    32521 cacgccgcat ccggcatccg agcaccattg cctgatgcga cgcttacgcg tcttatcagg
    32581 cctacaaacc tgtgcctgaa ccgtaggccg gataaggcgt ttacgccgca tccggcagtc
    32641 atgcgtcgat gccaaatgcg ccgccctaaa gcagcgcatc cgcaatgacg tacttattcc
    32701 ttcggtttaa accgcagcaa ccggttggcg ttacttacta cggtaattga cgagagcgcc
    32761 attgccgctc cggcaactac cgggttaagc agtgttccag taaacggcca caaaataccg
    32821 gcggcgaccg gaataccgat gctgttgtag ataaaggcac cgagcaggtt ctgcttcatg
    32881 ttgcgcagcg ttgcgcggga aatcgcgagc gcatccgcaa cgcccatcag gctatggcgc
    32941 atcagggtaa tcgccgcggt ttcaatggca acatcactgc cgccacccat cgcaatgccg
    33001 acatccgcct gggccagcgc tggcgcgtcg ttaatgccgt cgcccaccat tgccacctga
    33061 cgtccttcac tttgcagacg tttgatcgct tcggctttac cgtccggcag cactccggca
    33121 atcacttcat caattccagc ttctttggcg atcgcattgg cggtggttgg gttatccccg
    33181 gtcaacatca ccagacgata tcccgcttta tgcaggcgtt gcagcgccgc cacgctatca
    33241 ctacgcaacg gatcgcgtac agccagcagg gctactgctt tcccgtcaat cgccagcagc
    33301 acaggcgttg ctccttgcga tgcctgagct gaaatatcgg cttcaatcgc tttagtatcg
    33361 acttgctgat catttaacaa cgcctgatta cccagcaata acgcatgacc ttctgcttca
    33421 ccgctcacgc ccagcccgcg caatgtgcgg aaaccgttga cctgcggtag ctgcatatcg
    33481 cctgctttat caaggatcgc tcgtgccaat ggatggctgg aaccttgctc cagcgccgcc
    33541 gccagacgca gcgcctgcgc ttcgtcaaca tcagcaaatg ttttcactgc gacaacctgc
    33601 ggcttccctt cggtcagcgt cccggtttta tcgaacacga cggtatcgag tgtactggcg
    33661 cgttgcagcg cgtcggcgtc ccgcaccagc acgccaaact cagccgcccg cccgacgccg
    33721 gaaataatcg acatcggcgt cgccagtccc agcgcacacg ggcaggcgat aatcagcacc
    33781 gtagtggcaa tcaccagggt atagacaatc tgcggtgccg gaccaaagaa ataccagatt
    33841 gccgcactga caagcgcaat aaccactact accggcacaa atacggctga gattttatcc
    33901 gccagctgac cgatttctgg cttgctgctc tgggcctggc gcaccatgcg aatgatcctt
    33961 gatagcgtgg tatggctgcc aaccgcactg gcacgaaaca gcacactgcc gtcctgtacc
    34021 actgtcccgg catggacgct atcgccttcg cctttttgct gcgggattgg ttcgcccgtc
    34081 agcatcgctt catccagcca tgcttcgccc tgggtaatct cgccatctac cggcacgcga
    34141 tcgccggtcg tcaggcgcag caacatacct ggctgcactt ctgccagagg cacgcttttt
    34201 tcaccttcgt cagtaaccag gcgcgccgtc ggtggggtta aatcgagtaa tttctccagt
    34261 gccttagaag aacgctgacg tgcgcgtgct tccagcatat ggccgagatt gatcagaccg
    34321 ataatcatcg cactggcttc atagtaaaga tgtcgcgctt ccatcgggaa ccactgcggc
    34381 cacaggttga cgctcatcga atagagccac gccacgcccg ttcccagcgc caccagcgta
    34441 tccatcgtcg ctgcaccgtt cagcaggctt ttccatgcac tgcggtaaaa atggccgctg
    34501 gcgaaaacca tcaccgccag ggttatcagg ccgataacca accacaggct gcggttgtcg
    34561 gcggtgacca tcatgttatc gccgatcatc ccccagacca tcaccgggat acccaccgcc
    34621 agtgcgacaa ttgcctgcca gcggaagcgc ttcatcgtag cgacggcggt ttcttgctgg
    34681 cgttcgcggc gtttcgcgtc atcttcaatc gcttccgcgc cgtagcccgc tttttccacc
    34741 gcctgcacta aatcttgtgg ggaggcactg cccatcacca gcgcagtacg ctccgccagg
    34801 tttacccgtg cctgagtgac gcccggtacg ctttgcagcg cattttgtac gcgggtgaca
    34861 cagctggcgc agctcatgcc gctcagcagc aactgctggc tgtcatcgtc atcggcggtc
    34921 gctgccggaa gcgcctcaga aaccgctgtc agtgcttccg acgggattga tgactccgcc
    34981 agcggtttag cctttgggtg gcttacagat gcgtcataac ccgcttgttt gatggtttcg
    35041 atcagctgtt ctgcactggc agtcccggta acgtgcgctt cagtgataga cacatccgct
    35101 tgttccacat ccgggcgctg ttcaagactt tctttcacgc gtttaacgca gtgaccgcag
    35161 gacaggccgt ccaggttcag gtcgatagtt tgtgacataa aacactcctt taagacagtt
    35221 ttgactggct gtgataaagg ttaaaccttc cagcaagggg aaggtcaaga aattaataaa
    35281 ccaggcgggt aaaagtccgt aaagattaaa aaatcggctc gatttgcatc aggattagac
    35341 atttatttct ttgttttcct gtagttaagt tgcgggtgct aagttaaatc aggatgcctg
    35401 aaaatcggca ctggggtgag gaattacctc ccgcatctat aaaaaggagt taacaaaaga
    35461 tgttagatgc aaacaaatta cagcaggcag tggatcaggc ttacacccaa tttcactcac
    35521 ttaacggcgg acaaaatgcc gattacattc cctttctggc gaatgtacca ggtcaactgg
    35581 ctgcagtggc tatcgtgacc tgcgatggca acgtctatag tgcgggtgac agtgattacc
    35641 gctttgcact ggaatccatc tctaaagtct gtacgttagc ccttgcgtta gaagatgtcg
    35701 gcccgcaggc ggtacaggac aaaattggcg ctgacccgac cggattgccc tttaactcag
    35761 ttatcgcctt agagttgcat ggcggcaaac cgctttcgcc actggtaaat gctggcgcta
    35821 ttgccaccac cagcctgatt aacgctgaaa acgccgaaca acgctggcag cgaattttac
    35881 atatccaaca gcaactggct ggtgagcagg tagcgctctc tgacgaagtc aaccagtcgg
    35941 aacaaacaac caacttccat aatcgggcca ttgcctggct gctgtactcc gccggatatc
    36001 tctattgtga tgcaatggaa gcctgtgacg tgtacacccg tcagtgctct acgctcatca
    36061 atactgttga actggcaacg cttggcgcga cgctggcggc gggtggtgtg aatccgttga
    36121 cgcataaacg cgttcttcag gccgacaacg tgccgtacat tctggccgaa atgatgatgg
    36181 aagggctgta tggtcgctcc ggtgactggg cgtatcgcgt tggtttaccg ggcaaaagcg
    36241 gtgtaggtgg cggtattctg gcggtcgtcc ctggcgtgat gggaattgcc gcgttctcac
    36301 caccgctgga cgaagaaggc aatagtgttc gcggtcaaaa aatggtggca tcggtcgcta
    36361 agcaactcgg ctataacgtg tttaagggct gatcatgatg aacacggaag gtaataacgg
    36421 taacaaaccc ctcggtctat ggaacgtcgt ttccatcggt attggggcaa tggtgggggc
    36481 ggggatcttc gcgctgctgg ggcaggctgc gttgctaatg gaagcctcga cctggggcgc
    36541 ctttgctttt ggcggtattg tggctatgtt ttccggttat gcctatgcgc gtctgggggc
    36601 gagctatccc agcaatggcg gtattatcga cttttttcgt cgcggattag gcaacggcgt
    36661 cttttcgcta gcgctctcgt tgctatacct gttgacgctg gcggtgagca tcgccatggt
    36721 tgcccgtgct tttggcgctt atgccgtgca gtttttgcat gaaggcagcc aggaggagca
    36781 ccttattttg ctctacgcgt tggggatcat tgcggtgatg acgcttttca actccttaag
    36841 caaccatgcg gtagggcggc tggaagtgat cctcgtcggc attaaaatga tgatcctgtt
    36901 attgctgatt attgccggtg tctggtcgct gcaaccggcg catatttccg tctctgcgcc
    36961 ccccagctcc ggtgcgttct tctcctgtat tgggataacc ttccttgcct atgcgggctt
    37021 tggcatgatg gcgaacgcgg cggataaagt gaaagatccg caggtcatta tgccacgggc
    37081 gtttctggtg gcgattggcg ttaccacgtt gctttatatc tcgctggcgc tggttttgct
    37141 tagcgatgta tcggcattag agttagaaaa atatgctgat accgccgtag cgcaggctgc
    37201 ttctccgctg ctcgggcatg tgggttatgt gatcgtcgtc atcggcgctt tactggcgac
    37261 ggcttcaggc attaacgcga acctgttcgc cgtgtttaac atcatggaca acatgggcag
    37321 cgaacgcgaa ctgccgaagc taatgaataa atccctgtgg cggcagagta cctggggcaa
    37381 tatcatcgtc gtggtgttga taatgctgat gacggcggcg ctaaatttag actcactcgc
    37441 cagcgttgcc agcgccacct ttttgatctg ctatctggcg gtgtttgtgg tggcgatccg
    37501 cctgcgtcat gatattcacg cctcgttgcc gattcttatc gttggtacgt tggtgatgtt
    37561 gttggtgatc gttggcttta tctacagcct gtggtcccag ggcagccgtg cgttgatatg
    37621 gattattggt gcaatcttac tcagccttat tgtggcaatg gtcatgaagc gtaataaaac
    37681 cgtataacac ctctctgtgc gcagtacttc ctgtattatt gtggtggcgg tcgatattcg
    37741 cactggcaaa aaaacgtgct tgaatatctg ttgaaaccct ttaacaaagc acaggaggcg
    37801 ttgcgcgaac gatgaacatc agcgatgtag caaaaattac cggcctgacc agcaaagcca
    37861 ttcgcttcta tgaagagaag gggctggtga cgccgccgat gcgcagcgaa aacggttatc
    37921 gcacctacac gcagcagcat ctcaacgaac tgaccttact gcgccaggca cggcaggtgg
    37981 gctttaacct ggaagagagc ggcgagctgg tgaatctgtt taacgacccg cagcggcaca
    38041 gcgccgacgt caaacggcgc acgctggaga aggtggcgga gatcgaacga cacattgagg
    38101 agctgcaatc catgcgcgac cagctgctgg cactggcgaa tgcctgccct ggcgatgaca
    38161 gcgccgactg cccgattatc gaaaatctct ccggctgctg tcatcatcgg gcagggggat
    38221 taagacgaga cggctcggat atgtagggtt atcccttcta tcgcaatgac ttcaacatgc
    38281 gtacctgcac cgagatcctc gctggcgctg acaggccatg aactgtcacc gacgcgcata
    38341 tgaccgcgcc cgttgaccag cggagattcc agcacaaaac gtcggccaat cagctgctgc
    38401 ccgcgctggt ttaaatgact atcgctgtgc ttttgttcgc gcacccgccg cgacaaccat
    38461 ttccaccaca gccaggcggc gagcagcgtc aggatggcaa acattacccc ttgccactcc
    38521 caacccagcg gcaccagcca gaccaccagg ccggtaatca ccgctgccac gccgctccac
    38581 aacaaataac catttccgcc cagcatctcg gctgccagca gcaacccgcc gagactgagc
    38641 cagaaaatat gtggatgaac gaccattaac tccatcatgg ctgagtccgt ttgttggcgc
    38701 tgtctttcac cagctcggca atcccggcaa tcgaccccat caggctgctg gcctctaatg
    38761 gcatcatcac tactttgctg ttactggagg aaccgatctg ctgtaacgct tcggtgtatt
    38821 tttgcgctac gaagtagttc accgcctgaa tatcaccgga ggcgatagct tcagacacca
    38881 ttttggtggc gcgggcttct gcttcggcgg aacgttcacg cgcttcagcc tgtaaaaacg
    38941 ccgactgacg ttcgccttcc gctttcagga tttgcgactg tttttcacct tcggctttga
    39001 ggatttccgc ctgacggatc ccttccgctt caagaatgta agcgcgtttg gtacgttccg
    39061 ctttcatctg cgcgttcatt gaagagataa gctctgccgg tgggcgcacg tcgcgaattt
    39121 caatacgggt gactttaatc ccccacgggt tggtggcttc atcgacaata cgcagcaggc
    39181 gtgagttgat gctgtcgcgc tgagagagca tttcgtcaag ttccattgaa cccaacacgg
    39241 tacggatgtt agtcatggtc aggttgatga tcgccagctc cagattgctg acttcatagg
    39301 cagcgcgtgg cgcgtcaatc acctgaataa aacagactgc gtcgatggta acgttggcgt
    39361 tatctttcga gataacttcc tgggaaggga tatcgagcac ttgctccatc atattgatct
    39421 tgcgaccaat gcgatccata aacggcacca ccagactgag ccccggctgt aacgttttgg
    39481 tatagcgacc aaaacgttct accgtccatt gatagccctg cggcacgatt ttgacgcccg
    39541 cgccgacaat gaccagcgcg acaaaaatga gaatcgggat aaagataagc atcggaaaaa
    39601 cctcctgttg taccgtccat aatcagcaaa attgctgctt gattaaacaa attatacctg
    39661 attactgaaa gagagttccc ccttattcct gcgaaggata aactgttttt agtaaaaatc
    39721 agaaaaaggg aacagcgatg caggaaaata gtcctttgct tcagctacaa aacgtaggat
    39781 atctggcggg tgatacgaag attcttaata acatcaattt ttcgctgcgt gctggcgaat
    39841 ttaagttaat taccggtcct tctggttgtg gcaaaagtac gctgctaaaa atagttgctt
    39901 cattgatcag cccaaccagc ggaacgttac tgtttgaagg tgaggatgtc agcacactaa
    39961 aaccagaaat ctaccgccag caagtctctt actgcgccca gacgccgacg ctgtttggcg
    40021 atacggtata cgataatctg atctttccct ggcagatccg tgaccagcag cctgacccgg
    40081 ccatttttct cgattttctc gaacgcttcg ctttgccgga cagcattttg acgaagaata
    40141 tcgccgagct atctggtggt gaaaaacaac gcatctcatt gattcgtaac ctgcaattta
    40201 tgccgaaggt tttattgctg gatgaaataa ccagtgcgct ggatgaaagt aataaacata
    40261 acgtcaatga gatgatccat cgttatgtgc gcgagcaaaa tattgccgtg ctgtgggtga
    40321 cacacgataa agacgaaatt aatcatgcgg ataaagtgat tacactgcaa ccgcatgccg
    40381 gagaaatgca ggaagcacgc tatgaactcg cataatatta ctaacgaatc attagcactg
    40441 gcattaatgc tggtggtggt ggcaatctta attagccata aagaaaaact ggcgctggag
    40501 aaagatattc tctggagcgt cgggcgagcg ataattcagc tgattattgt cggctatgtg
    40561 ctgaagtata ttttcagcgt ggatgatgcc agcctgacat tattgatggt gttatttatc
    40621 tgctttaatg cggcgtggaa cgcgcaaaaa cgcagtaaat atattgctaa agcttttatc
    40681 tcatcgttta ttgccattac ggtcggggcg ggaattaccc tggcggtgct gattctctca
    40741 ggggcgattg aatttatccc gatgcaggtg atccctgtcg ccgggatgat tgccggtaac
    40801 gccatggtag cggtggggtt gtgttacaac aatttagggc aacgggtcat tagcgaacag
    40861 caacagattc aggagaaact gagtcttggc gcgacgccga agcaggcttc agcgatattg
    40921 attcgcgaca gtattcgcgc ggctttaatt ccgacggtcg attcagcaaa aacggttggc
    40981 ttagtgagtt taccaggaat gatgtccggg ctgatatttg ccgggattga tccggtgaag
    41041 gcgattaaat atcagattat ggtgaccttt atgctgctct caaccgccag cttgtcgacc
    41101 attattgcct gctatttaac ctatcgtaag ttttataatt cgcgccacca gttggtggtg
    41161 actcaattga agaagaaatg atgcgatgcc ggatgcgcag catcgcatcc ggcgttgtgg
    41221 ttcatgtgcc gaacaacgga atatcaatac aacaatgcat acagctggcg gcgatacttc
    41281 gacgccagtg catcacccgt acccagcgca gcgaggatct cctggaacgt tttacgcgtc
    41341 tgaccgtctg cggcggtgag atctttacgc agatgcccga acagcaactc cagcgcctct
    41401 tcattgcgcc caacctgatg cagttgcagc gccagttgcg tcgccagtgc ggcatcttct
    41461 ggattctccg ccacctgctg ttgcaactgt tgaatttccg gcttatcagc cgcctgcttc
    41521 agcagttcga tttgcgctac cagcccctgg tagcgggtgt cctgatcctg caacggaatg
    41581 gttttcagca ccgcttccgc atcttcagaa cggttcagcg caatcagcgt ttctgccagc
    41641 agcaggccga tctccccgtt ctgattcgac aactgccagg cgtcttgcag caatggcagg
    41701 gcatcggtgt aattgccttc ctgcatcagt tgcatcgcct gctgcgcttt cagctcttct
    41761 tcgcgcggca gcactttatc cagcagggcg cggatcgcct cttccggttg cggcccctgg
    41821 aagccatcta ccggttgccc gttctggaac agatacacgg tcggaatcgc gcgcagacca
    41881 aactgggcgg caatcatctg ctccgcgtcg cagtccagct tcgccagaat aaattgcccg
    41941 ttgtactgcg ccgcgaggct ttccaggatc ggggttaact gcaaacagtg ctggctacgt
    42001 tcagaccaaa aatagaacag caccggagtg gtcatcgact gttcaagaac ctgttgcagg
    42061 ttagattcgt taatgttgac aatattttct acggacatgg agtcgctctc tgttgtcgat
    42121 attttctttg acatgggggc ttaagcgcgc gcttcaactc acccctgcaa tattttgtcc
    42181 atcacgcgcc caggcagcag gcgctcgagc accattactg cccaggtcac cagcgttacc
    42241 ggatagcgca tcttcggctt ctcgctaata aaagcatggc gcactttgtc gaccaccgct
    42301 tccggtccca acgtaaagcg ggcggcgatg ccgggatttt cgactggttt atcactttgc
    42361 gtctggttga cgttgtcggt gaagcgagta cgaatgggac cgggttcgat caggctgact
    42421 ttaattccgc tgtggcgtag ctccatgcgc agcgcgtctg accacgcctc cagcgcatat
    42481 ttactggccg cgtaagcgcc acgacccggc gtggagatta atcccatcac cgatgatgtc
    42541 atcacaatac gcccttcacc gtgcggtaac atcgcgggta acaggcgcat ggtgagctgg
    42601 tgtgcgccga aaaagttggc ggaaaactgc tgttccatct gcgcacggct gatggtggaa
    42661 agggggccat acatgccgaa tccggcattg ttaaagatcc catacagaca attatcggtc
    42721 agggcgatca cctcgtctgc tgcgcgatta acactttctg gtgaatccag atcgatcaac
    42781 acgccggtaa atcccatgtt gttcatacgc tcaacatcat tcggttttcg gcaacctgcc
    42841 agcacatgaa aaccctggcg ttttaattcg agcgcgcttt ccaggccaat tccactggaa
    42901 catccggtaa ttaagaccga tttttgcata actttacctg tcaggatctc cgttgcttta
    42961 tgagtcatga tttactaaag gctgcaactg cttcgccatc cagtcggcaa taaacggctg
    43021 ggcgtcgcgg ttgggatgaa taccgtcatc ctgcatccat tgtggcttga ggtagacctc
    43081 ttccataaaa aagggcagca gcggaacatc aaactctttg gcgagtttgg ggtaaatggc
    43141 gctaaaggct tcattataac ggcgaccata gtttgcaggc agacgtattt gcattaacaa
    43201 tggttcagcg ttggcggctt tgacatcctg caaaatctgg cgcagcgttt gctcggtttg
    43261 ctgtggctga aaaccacgca aaccatcatt gcgcccagtt caaccagcac ccaacgcggc
    43321 tgatgctgtt tgagcagagc cggaaggcgc gccagccctt gttgcgaggt gtcgccgctg
    43381 atgctggcat taactaccga cgttttactc tgccacttat cattcaacaa ggcaggccag
    43441 gccgcgctgg cagacattcg atacccggcg ctcaggctat cacccagaat caataacgtg
    43501 tccgctgcgg cggcacggaa ggttaacagg accaggaaca ggaagggcaa atgccagcgg
    43561 aaaacattgt tgaagttcat catcttaaga agtccgtcgg tcagggggag catgaactct
    43621 ccatcctcac cggagttgag ctggttgtca aacgtggcga gaccatcgca ctggtgggcg
    43681 agtcgggatc gggtaagtca accttgctgg cgatcctcgc cgggcttgat gacggcagca
    43741 gtggcgaagt gagtctggtg ggacaaccgc tacataatat ggacgaagaa gcgcgggcaa
    43801 agttgcgcgc gaagcacgtc ggctttgttt ttcagtcatt tatgttaatt cctaccctta
    43861 acgcgctgga aaacgtcgag cttccggctc tgctgcgcgg tgagagtagc gcggaaagtc
    43921 gtaacggggc gaaagcgttg ctcgaacagt tagggctggg taaacgtctg gatcatcttc
    43981 cggcacagct ttccggcggt gaacagcaac gagtggcgct ggcacgagcc tttaatggtc
    44041 gacctgatgt gctgtttgcc gacgaaccca ccggcaacct tgaccgccag acgggcgata
    44101 aaattgccga cctgctgttt tccctcaacc gtgaacatgg caccacgttg attatggtga
    44161 cccacgacct gcaactggcg gcacgctgcg accgctgctt acggctggtg aacgggcagt
    44221 tgcaggagga agcatgattg cacgttggtt ctggcgcgaa tggagttcgc cgtcgctatt
    44281 aattgtctgg ctggcgctaa gcctggcggt ggcctgcgtg ctggcgctgg gcaatatcag
    44341 cgatcgcatg gagaagggct taagccagca aagccgtgag tttatggcgg gcgatcgggc
    44401 gttgcgcagt tcacgcgaag tgccgcaagc gtggctggag gaagcgcaaa agcgcggcct
    44461 gaaagtcggc aagcagctga ctttcgccac aatgaccttt gcaggcgaca caccgcagct
    44521 ggcgaacgtc aaagcggtgg atgatatcta cccgatgtat ggcgatctgc aaactaatcc
    44581 ccctggcctg aaaccgcagg cgggcagcgt attgctggcc ccacgcctga tggcactgct
    44641 taacctgaaa acgggcgaca ccattgacgt gggcgatgcc accttgcgga ttgccggaga
    44701 agtgattcag gaaccggatt ccggttttaa ccccttccag atggctccgc gtctgatgat
    44761 gaatctggcg gatgtcgata aaaccggagc cgtgcagccg gggagtcggg tcacctggcg
    44821 ttataaattc ggcggcaacg agaaccagct cgacggctat gagaaatggt tgttacctca
    44881 gcttaaaccc gaacaacgct ggtacggtct ggaacaggac gaaggcgcgc tggggcgatc
    44941 gatggaacgc tcgcaacagt tcctgctgct ttcggcgctt ctgaccttgc tgctggcagt
    45001 ggcagcggtg gcggtagcga tgaatcatta ctgtcgcagt cgctacgatc tggtggcgat
    45061 cctcaaaacg ctgggggcag ggcgagcgca actgcgtaag ctaatcgtcg gtcagtggtt
    45121 gatggtgctg acgctttcag ccgttaccgg tggggccata ggcctgttgt tcgaaaacgt
    45181 gttgatggtg ctgctcaagc ccgttctgcc tgctgcacta ccgccagcca gcctctggcc
    45241 gtggctgtgg gcgcttggca ccatgacggt catctcgctg ctggtggggc tacgaccata
    45301 ccgcttgttg ctggcaacgc agcctttacg cgtattacgt aatgatgtgg tcgcgaacgt
    45361 ctggccgctg aagttttatc tgccgattgt cagtgtggtg gttgtgctgc tgctcgccgg
    45421 attaatgggt ggcagcatgc tgctttgggc ggtgctggcg ggcgcggtag tactggcttt
    45481 gctgtgcggt gtgctgggct ggatgctgct gaatgtactt cgccgcatga cgctgaaatc
    45541 gctgcctctg cgcctggcgg ttagccgcct gttacgtcag ccgtggtcaa cgttaagcca
    45601 gctttcggca ttttcgctct cctttatgct gctggcactg ctgctggtgc tgcgtggcga
    45661 tctgctcgac cgctggcagc agcagctacc gccagaaagc ccgaactact ttttaattaa
    45721 catcgcccac agaacaggta gcaccgctaa aagcgttcct cgcggaacat catatagtcc
    45781 cggaatcatt ttatccggta gtgcgggcgc ggctgacggc gattaatgat aagccgacag
    45841 aaggtaatga agatgaggcg cttaatcgcg aactcaatct tacctggcaa aatacgcggc
    45901 ccgatcataa tccgattgtc gccggtaact ggccgccaaa agccgatgaa gtgtcgatgg
    45961 aagaggggct ggcgaaacgc ttaaacgttg ccctcggtga taccgtgact tttatgggcg
    46021 atacccagga gttccgcgct aaagtgacca gcctgcgcaa agtggactgg gaaagtctgc
    46081 ggcctaattt ctattttatt ttccctgaag gggcattaga cgggcaaccg cagagctggc
    46141 ttaccagttt ccgctgggag aatggcaacg gcatgttgac gcaactcaac cgccagttcc
    46201 cgaccattag cctgttagac attggcgcga ttttaaaaca ggtcggtcag gtgctggagc
    46261 aggtaagtcg ggcgctggag gtgatggtgg tgctggtcac cgcctgcggt atgttgctgt
    46321 tgctggcaca ggtgcaggtg ggaatgcgcc agcgtcatca ggagctggtg gtatggcgca
    46381 cactcggtgc ggggaaaaaa ctgctgcgca cgacattgtg gtgtgatgtt cgccatgctt
    46441 gggtttgttt ccggcctggt ggccgcaatt ggtgcggaaa cggcactggc agtgttgcag
    46501 gcgaaagtgt ttgatctccc gtgggagcca gactggcgat tgtggattgt tctgccgtgc
    46561 agcggtgcgc tgctgctgtc gcttttcggc ggctggctgg gtgcgcgact ggttaagggt
    46621 aaggcgctgt tcaggcagtt tgtggggtga tgacaggtgt tagattgcct gatgcgctac
    46681 gcttatcagg cctacgaaga gcgtgcaata tattgatttt gcaagattgt gtaggccgga
    46741 taaggcgttt acgccgcatc tggcatgaac agtgtgcatt ttggcaataa atatgaaacc
    46801 aactgtcatg tcggtttttt taaaggccga aggaggtaaa ccttcggcct tagctacgtt
    46861 tcctcagtta ttcaataaac tgacggatca gttccagcag cgcgattagc gccttaagaa
    46921 cgatgagaac tttatcgaga aaagttttca tccgttgctc ccttaaggga gaaagacaat
    46981 cacagcttac ccttacgttg cctataacta tgattttctt catgtgctag atgaggactg
    47041 tctctctcca aattagagca ccagaatcag caccacctgt atcctgacca ggacttaaaa
    47101 gtacttttcc gcactgcggt aatcccttga ggcagtatca ctactagcgt cgggattgta
    47161 tcgccaacat agctgtttga atatgtaggc atcctgactt ataattcact tgctagatga
    47221 ggactgttta atttctgccc ggaaccacca agtcttgggc ggaaaaaata aaaaaaccga
    47281 ctgtcatgtc ggttttttat atctgtattc tggtgcaacc attttgttct cacgacaatc
    47341 gccgtttcag aataccgccc atcactgcgc cttaacccat accgccactt ccgcccactc
    47401 accgcgaaag acaacttttt ccgctttttt ctcaagctga tagcgataca ttgggtcgta
    47461 atattcttta agtaacggca ccagccaggc cagatgaccg tcggtgctgc cggtggtgag
    47521 ttgcgttgtc agcgccgtat ccagctgggc agcgagttcg ttatagcgct gtaaccccag
    47581 ccgacgctta atcgccgaaa gtccgtgatg caggtattcg caatattcct gccagccctg
    47641 ttcgtcgcca taagcgtggg taaaatcatg atgcatacgc aagaaatact cttcattcag
    47701 gcgttcaaga cggatctcaa acggatcttc taccaccgca atcgccgcct gagtcattcg
    47761 ctcgcgcaga cattccggca ggtgattcga accgatcatc cggctttcat cttccagcac
    47821 ccacaggcgc aaatcctgac gggcgtcggt ttttagcatt tcggcagcca gcaggttttc
    47881 aaaactcgcc tggctaagtt gtggttgtaa cgtgcgacca aacgccgaac cgcgatgacg
    47941 cgccagccct tccagatcaa caccgtttgg ctgttgttgc actaatagcg ttttaccgct
    48001 gccggtacaa ccgccaatca gcactatcgg tttttgtgcc agttcaatgg tcgcctgaat
    48061 cgcggtctgg cgcagtgcct tataaccgcc ttccaccagc ggataatcaa tccccgcttc
    48121 atgcagccag cgttgcacaa tatgtgaacg ttgaccgcca cgggcgcagc agagaatacc
    48181 ttgcggattt tgcaggcacg ctgcccgcca ggcgtacata cgctgctgac gaatttcacc
    48241 cgccaccagt ttatgcccca gcgccagcgc tgcgtctgag ccttgctgtt tatagcaggt
    48301 gccaacggag gcgcgttcat cgttatccat taacggcaga ttgatagcgg cgggcattgc
    48361 gccttgctca aactcgatag gggcgcgaac atcaataatg ggcgtatcag caatcagcag
    48421 ggcacgatag tcctgttccg tgtgtctctc ttgcatagtt aaaagtgaac ctcaaatcag
    48481 cttgcgcgct attttacgcg ccaacgcgca aggaaacttg atttttaact gcgtggggtg
    48541 ccgaaaattt ctaaaaatcc gctgatttcc ggcctgtgtt gggtaaacaa ggtcacaata
    48601 tcttctaccg ctttgcagct gccaaatttg cgccatgcca gactcaatgg cgaaggaggg
    48661 cgcatcgttg ggattacccg gctgaccagt tgttgattat cgatcattga ctggcaaagt
    48721 gattttggca aaaaaccaat gccaacaccc gccagatggg cggcgatttt cgtttccata
    48781 tcgggaacaa taatctcttt ttgccccggc aatcgccagg cgacgcgttt ggttaaggtg
    48841 ccggcgctgt cttcaatatt gaccgccgga aagcgacgca actgcgcttc tgttagcggc
    48901 tcttcaacgt tcgccagcgg atgatccgcc gccatgacaa atcgccattg caccgatcct
    48961 aagggatcaa gactaaaggt atttgccagc gcctcagttc ccgtgacccc gatagccagc
    49021 gaaaaacctt cgtacaatag cgagtcccag acgcccatat agatttgtcg ggagatgtga
    49081 aactgggtaa aggggtaacg ctcattcagc cacgccagca actgggcgac ggcctggggg
    49141 ttatagagca ggttgttgat gacaatattc acctggcgtt ccacgccatc attcacctgt
    49201 tgcagctcgc tgggcatact ttccagccag ctcagccagt ctctggcctg ggaaagtaga
    49261 tgctcgccag ccgctgtcaa cgttacgcta cgagtcgtac ggaaaaacag cgctactccg
    49321 gtattctctt ccagaagttt aatgcgataa ctgatcgtcg ccgtggtttt acataatcgt
    49381 tctgccgctt ttgaaaaact tcctgtttca gcaaccgcaa tgaaagtccg caaggtttct
    49441 ggatcgaaca tcttcaggta tcccctttta aatccgcaag ttgcgtgatt ttcttatcct
    49501 ctgatttatc agtattttta catgataatc ctgttcaatt tgtggactaa atctagtttt
    49561 ggaaaaacat tccaactttt gtattgatgt tgttctctta aggttttaga ttgcctgtta
    49621 ttgaaaccaa gctgaccggt cggcggtggt tgaacggaat tatgttacaa ggacaaaaag
    49681 atgaaacttc aggtattacc gttaagtcag gaagccttta gtgcttatgg cgacgtaatc
    49741 gaaacgcagc aacgggattt tttccatatt aacaatggcc tggtggagcg ttaccacgat
    49801 ttggcgctgg ttgagattct tgagcaagac cgtacgctta tcagcattaa ccgcgcgcaa
    49861 ccggcgaatc tgccgctgac cattcacgaa ctcgaacgtc atccgctggg cactcaggcc
    49921 tttatcccaa tgaaaggtga agtgtttgtg gtggtcgtgg cgttaggcga cgacaaacca
    49981 gacctgtcaa cgctgcgggc gtttatcacc aacggtgaac agggggtgaa ttaccatcgt
    50041 aacgtctggc atcacccact tttcgcctgg cagcgcgtca ccgattttct gaccatcgat
    50101 cgcggcggca gtgacaactg tgatgttgaa agtattcctg aacaggaact ctgttttgcg
    50161 tgacgcctgc aaccgacttg cataagataa actaattgtt cattgtttat gctcacttgt
    50221 aggtcggagt taacgtaggt atgacggaag ttagacggcg cggcaggcca ggacaggcgg
    50281 agcctgtggc acagaagggc gcacaggcgt tagagcgggg aattgcgatt ctgcaatatc
    50341 tggaaaaaag tgggggaagt tcgtcggtta gcgatatttc tctcaatctg gatttgccgc
    50401 tctccacgac ctttcgcttg ctgaaggttt tacaagcagc ggattttgtc tatcaggaca
    50461 gtcaattagg ctggtggcat ataggattag gtgtctttaa cgtcggtgcg gcgtacatcc
    50521 ataaccgcga tgtcctctcc gtcgccgggc cgtttatgcg ccgcctgatg ttactttccg
    50581 gcgaaacggt caatgtcgcg atccgtaacg gcaatgaagc ggtattaatt ggtcagttag
    50641 agtgtaaatc gatggtcagg atgtgtgcgc cactgggcag tcgtctgccg ctgcatgctt
    50701 ccggtgcggg caaagcgctg ctttatccgc tggcggaaga ggagttgatg agcatcattc
    50761 tgcaaaccgg tttgcagcag tttacgccaa ctacgcttgt ggatatgccc accttgctga
    50821 aggacctgga acaagcgcgt gaactgggct ataccgtaga taaagaagag catgttgtag
    50881 gtctgaattg catagcttca gcaatttacg atgatgtcgg tagagttgtt gccgctatct
    50941 ccatctccgg gccttcatca agactgacag aagatcgttt tgtcagtcag ggtgagctgg
    51001 tcagagacac cgcccgcgat atcagcacgg cgttgggact gaaagcacat ccatagtgtc
    51061 tgtcgcatcc cgctctgcgg agcgggtttt tttgacaaaa tttgaaagtt ggaaaatttt
    51121 tccaattaat agaggtagga ataaaatggc aaaaatgaga gccgttgacg cggcaatgta
    51181 tgtgctggag aaagaaggta tcaccaccgc cttcggcgtt ccgggagctg caattaatcc
    51241 gttctactca gcgatgcgta agcacggcgg tattcgtcac attctggcgc gtcatgtgga
    51301 aggtgtttcg cacatggcgg aaggttatac ccgcgcaacg gcagggaata tcggcgtatg
    51361 tctggggact tccggtcctg cgggcacgga catgatcacc gcgctctatt ccgcttctgc
    51421 tgattccatt cctattctgt gcattaccgg ccaggcaccg cgcgcccgtc tgcataaaga
    51481 agattttcag gccgtagata ttgaagcaat tgctaaaccg gtcagcaaaa tggcggttac
    51541 agttcgtgaa gcggcgctgg tgcctcgcgt gctgcaacag gcatttcacc tgatgcgttc
    51601 tggtcgtccg ggtccggtac tggtggattt accgttcgac gttcaggttg cggaaatcga
    51661 gtttgatcct gacatgtacg aaccgctgcc ggtctacaaa cctgctgcca gccgtatgca
    51721 gatcgaaaaa gctgtagaaa tgttaatcca ggccgaacgt ccggtgattg ttgccggggg
    51781 cggggtaatc aatgctgacg cagctgcact gttacaacag tttgctgaac tgaccagcgt
    51841 tccggtgatc ccaacgctga tgggctgggg ctgtatcccg gacgatcatg aactgatggc
    51901 cgggatggtc ggtctgcaaa ccgcgcatcg ttacggtaac gcaacgttgc tggcgtccga
    51961 catggtgttt ggtatcggta accgttttgc taaccgtcat accggttcgg tagagaaata
    52021 caccgaaggg cgcaaaatcg ttcatattga tattgagccg acgcaaattg gtcgcgtgct
    52081 gtgtccggat ctcggcattg tctctgatgc taaagcggcg ctgacactgc tggttgaagt
    52141 ggcgcaggag atgcaaaaag cgggtcgtct gccgtgtcgt aaagaatggg tcgccgactg
    52201 ccagcagcgt aaacgcactt tgctgcgcaa aacccacttc gacaacgtgc cggtgaaacc
    52261 gcagcgcgtg tatgaagaga tgaacaaagc ctttggtcgc gatgtttgtt atgtcaccac
    52321 tattggtctg tcacaaatcg ctgcggcaca aatgctgcat gtctttaaag accgccactg
    52381 gatcaactgt ggtcaggctg gtccgttagg ctggacgatt ccggcggcgc tgggggtttg
    52441 tgccgctgat ccgaaacgca atgtggtggc gatttctggc gactttgact tccagttcct
    52501 gattgaagag ttagccgttg gcgcgcagtt caacattccg tacatccatg tgctggtgaa
    52561 taacgcctat ctcggcctga ttcgtcagtc acaacgcgct tttgacatgg actactgcgt
    52621 gcaactcgct ttcgagaata tcaactccag tgaagtgaat ggctacggtg ttgaccacgt
    52681 aaaagtagcg gaaggtttag gttgtaaagc tattcgggtc ttcaaaccgg aagatattgc
    52741 gccagccttt gaacaggcga aagccttaat ggcgcaatat cgggtaccgg tagtcgtgga
    52801 agttattctc gagcgtgtga ccaatatttc gatgggcagc gaactggata acgtcatgga
    52861 atttgaagat atcgccgata acgcagcgga tgcaccgact gaaacctgct tcatgcacta
    52921 tgaataaggg agataaatga tgttacgttt ctctgctaat ttatcgatgt tatttggtga
    52981 atatgatttt ctcgcccgtt ttgagaaagc tgcgcagtgt ggttttcgcg gcgttgaatt
    53041 tatgtttcct tatgactacg acattgaaga attaaaacag gtactggcga gtaataaact
    53101 cgaacatacg ctgcacaatt taccggcagg tgactgggcg gcgggcgagc gcggtattgc
    53161 ctgtattcct ggccgtgaag aagagtttcg ggatggcgta gcagcagcga tt
//
LOCUS       DAJTQY010000018        52077 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.018, whole genome shotgun sequence.
ACCESSION   DAJTQY010000018 DAJTQY010000000
VERSION     DAJTQY010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 52077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52077
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.018"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(262..2541)
                     /gene="maeB"
                     /locus_tag="OO349_001091"
     CDS             complement(262..2541)
                     /gene="maeB"
                     /locus_tag="OO349_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HCR5866251.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGVQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            2830..3780
                     /gene="tal"
                     /locus_tag="OO349_001092"
     CDS             2830..3780
                     /gene="tal"
                     /locus_tag="OO349_001092"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCR5866252.1"
                     /translation="MNELDGIKQFTTVVADSCDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGCVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     TQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            3800..5803
                     /gene="tkt"
                     /locus_tag="OO349_001093"
     CDS             3800..5803
                     /gene="tkt"
                     /locus_tag="OO349_001093"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCR5866253.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFPRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQMEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(5906..6949)
                     /locus_tag="OO349_001094"
     CDS             complement(5906..6949)
                     /locus_tag="OO349_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HCR5866254.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQIIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDARPTLWITR"
     gene            complement(7075..7650)
                     /gene="nudK"
                     /locus_tag="OO349_001095"
     CDS             complement(7075..7650)
                     /gene="nudK"
                     /locus_tag="OO349_001095"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HCR5866255.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKSVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQMSHLMD"
     gene            complement(7718..9697)
                     /gene="aegA"
                     /locus_tag="OO349_001096"
     CDS             complement(7718..9697)
                     /gene="aegA"
                     /locus_tag="OO349_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HCR5866256.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKFGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDLLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVLIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            9903..11620
                     /gene="narQ"
                     /locus_tag="OO349_001097"
                     /pseudo
     CDS             9903..11620
                     /gene="narQ"
                     /locus_tag="OO349_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011308.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
     gene            11822..14935
                     /gene="acrD"
                     /locus_tag="OO349_001098"
     CDS             11822..14935
                     /gene="acrD"
                     /locus_tag="OO349_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HCR5866257.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASSANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMLLTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVD
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(15034..15093)
                     /gene="ypfM"
                     /locus_tag="OO349_001099"
     CDS             complement(15034..15093)
                     /gene="ypfM"
                     /locus_tag="OO349_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HCR5866258.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            15474..15830
                     /locus_tag="OO349_001100"
     CDS             15474..15830
                     /locus_tag="OO349_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HCR5866259.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            15834..16961
                     /gene="dapE"
                     /locus_tag="OO349_001101"
     CDS             15834..16961
                     /gene="dapE"
                     /locus_tag="OO349_001101"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HCR5866260.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            16989..17189
                     /locus_tag="OO349_001102"
     CDS             16989..17189
                     /locus_tag="OO349_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HCR5866261.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(17299..17997)
                     /gene="ypfH"
                     /locus_tag="OO349_001103"
     CDS             complement(17299..17997)
                     /gene="ypfH"
                     /locus_tag="OO349_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR5866262.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASLVIALNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDQAL
                     SGGKPGDDDVIEMM"
     gene            complement(18071..20086)
                     /gene="tmcA"
                     /locus_tag="OO349_001104"
     CDS             complement(18071..20086)
                     /gene="tmcA"
                     /locus_tag="OO349_001104"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HCR5866263.1"
                     /translation="MAELTALYTLTAQMKREGICRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQLDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGRQLIAGALQYTHDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGEMLPVD
                     PLNDAILSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRITQWQFFH"
     gene            complement(20101..20964)
                     /locus_tag="OO349_001105"
     CDS             complement(20101..20964)
                     /locus_tag="OO349_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HCR5866264.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            complement(21132..21845)
                     /gene="purC"
                     /locus_tag="OO349_001106"
     CDS             complement(21132..21845)
                     /gene="purC"
                     /locus_tag="OO349_001106"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HCR5866265.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            complement(22058..23092)
                     /gene="bamC"
                     /locus_tag="OO349_001107"
     CDS             complement(22058..23092)
                     /gene="bamC"
                     /locus_tag="OO349_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HCR5866266.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            complement(23109..23987)
                     /gene="dapA"
                     /locus_tag="OO349_001108"
     CDS             complement(23109..23987)
                     /gene="dapA"
                     /locus_tag="OO349_001108"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HCR5866267.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLDLADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            24133..24705
                     /locus_tag="OO349_001109"
     CDS             24133..24705
                     /locus_tag="OO349_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HCR5866268.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     TFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            24705..25175
                     /gene="bcp"
                     /locus_tag="OO349_001110"
     CDS             24705..25175
                     /gene="bcp"
                     /locus_tag="OO349_001110"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HCR5866269.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            25428..26045
                     /gene="hyfA"
                     /locus_tag="OO349_001111"
     CDS             25428..26045
                     /gene="hyfA"
                     /locus_tag="OO349_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HCR5866270.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     ITAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAHAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGESPLSLTQEQH"
     gene            26045..28062
                     /gene="hyfB"
                     /locus_tag="OO349_001112"
                     /pseudo
     CDS             26045..28062
                     /gene="hyfB"
                     /locus_tag="OO349_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
     gene            28073..29020
                     /gene="hyfC"
                     /locus_tag="OO349_001113"
     CDS             28073..29020
                     /gene="hyfC"
                     /locus_tag="OO349_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HCR5866271.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLAMGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            29037..30476
                     /gene="hyfD"
                     /locus_tag="OO349_001114"
     CDS             29037..30476
                     /gene="hyfD"
                     /locus_tag="OO349_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HCR5866272.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVSTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLVFIGAMAGLVVSSTLLGQLLFFEITGGCSWALISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFVLSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISAYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMTLVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            30488..31138
                     /gene="hyfE"
                     /locus_tag="OO349_001115"
     CDS             30488..31138
                     /gene="hyfE"
                     /locus_tag="OO349_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HCR5866273.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            31143..32729
                     /gene="hyfF"
                     /locus_tag="OO349_001116"
     CDS             31143..32729
                     /gene="hyfF"
                     /locus_tag="OO349_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HCR5866274.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKP
                     PQAVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRS
                     TWHDFLPSGTASVSEKHSER"
     gene            32719..34433
                     /gene="hyfG"
                     /locus_tag="OO349_001117"
                     /pseudo
     CDS             32719..34433
                     /gene="hyfG"
                     /locus_tag="OO349_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
     gene            34443..34988
                     /gene="hyfH"
                     /locus_tag="OO349_001118"
     CDS             34443..34988
                     /gene="hyfH"
                     /locus_tag="OO349_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HCR5866275.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTH
                     KADLYTHATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPEC
                     KQRATLINDDTDVPLVAKEQL"
     gene            34985..35743
                     /gene="hyfI"
                     /locus_tag="OO349_001119"
     CDS             34985..35743
                     /gene="hyfI"
                     /locus_tag="OO349_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HCR5866276.1"
                     /translation="MSPVLTQYVSQPITLDEQTQKMKRHLLQDIRRSAYVYRIDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGTVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            35736..36149
                     /gene="hyfJ"
                     /locus_tag="OO349_001120"
     CDS             35736..36149
                     /gene="hyfJ"
                     /locus_tag="OO349_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HCR5866277.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVLEPAIYLMARKIA"
     gene            36179..38191
                     /gene="hyfR"
                     /locus_tag="OO349_001121"
     CDS             36179..38191
                     /gene="hyfR"
                     /locus_tag="OO349_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HCR5866278.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRYAVLLGSSGMAEL
                     RFIRTHGSRFTPQDCTLFNWQARIITPVLQSWLNDEAQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLSSVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PHNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADACRRMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRHDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRSIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGISVREVL"
     gene            38213..39061
                     /locus_tag="OO349_001122"
                     /pseudo
     CDS             38213..39061
                     /locus_tag="OO349_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
     gene            complement(39099..40160)
                     /locus_tag="OO349_001123"
     CDS             complement(39099..40160)
                     /locus_tag="OO349_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR5866279.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPVLMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            40373..41836
                     /gene="bepA"
                     /locus_tag="OO349_001124"
     CDS             40373..41836
                     /gene="bepA"
                     /locus_tag="OO349_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HCR5866280.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            41857..42216
                     /gene="arsC"
                     /locus_tag="OO349_001125"
     CDS             41857..42216
                     /gene="arsC"
                     /locus_tag="OO349_001125"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708333.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HCR5866281.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIMVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(42349..43095)
                     /gene="hda"
                     /locus_tag="OO349_001126"
     CDS             complement(42349..43095)
                     /gene="hda"
                     /locus_tag="OO349_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HCR5866282.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(43145..44434)
                     /gene="uraA"
                     /locus_tag="OO349_001127"
     CDS             complement(43145..44434)
                     /gene="uraA"
                     /locus_tag="OO349_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HCR5866283.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPNSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            complement(44520..45146)
                     /gene="upp"
                     /locus_tag="OO349_001128"
     CDS             complement(44520..45146)
                     /gene="upp"
                     /locus_tag="OO349_001128"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HCR5866284.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            45471..46508
                     /gene="purM"
                     /locus_tag="OO349_001129"
     CDS             45471..46508
                     /gene="purM"
                     /locus_tag="OO349_001129"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HCR5866285.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            46508..47146
                     /gene="purN"
                     /locus_tag="OO349_001130"
     CDS             46508..47146
                     /gene="purN"
                     /locus_tag="OO349_001130"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HCR5866286.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            47318..49384
                     /gene="ppk1"
                     /locus_tag="OO349_001131"
     CDS             47318..49384
                     /gene="ppk1"
                     /locus_tag="OO349_001131"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HCR5866287.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNAHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRSDSIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            49389..50930
                     /gene="ppx"
                     /locus_tag="OO349_001132"
     CDS             49389..50930
                     /gene="ppx"
                     /locus_tag="OO349_001132"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HCR5866288.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEILRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(50969..>52077)
                     /gene="pdeF"
                     /locus_tag="OO349_001133"
     CDS             complement(50969..>52077)
                     /gene="pdeF"
                     /locus_tag="OO349_001133"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HCR5866289.1"
                     /translation="KQKLSHWLSPLLEPGEDVYQLSGNDLALRLNTESHQERITALDS
                     HLKQFRFFWDGMPMQPQIGVSYCYVRSPVNHIYLLLGELNTVAELSIVTNAPENMQRC
                     GAMYLQRELKDKVAMMNRLQQALEHNHFFLMAQPITGMRGDVYHEILLRMKGENDELI
                     GPGSFLPVAHEFGLSSSIDMWVIEHTLQFMAENRAKMPAHRFAINLSPTSVCQARFPV
                     EVSQLLAKYQIEAWQLIFEVTESNALTNVKQAQITLQHLQELGCQIAIDDFGTGYASY
                     ARLKNVNADLLKIDGSFIRNIVSNSLDYQIVASICHLARMKKMRVVAEYVENEEIREA
                     VLSLGIDYMQGYLIGKPQPLIDTLNEIEPIRESA"
BASE COUNT        12157 a        13737 c        13522 g        12661 t
ORIGIN      
        1 cgtgacaatt tttcgtcaga gtaaataaca aaattcggca agcgcgttta aaaggtgagg
       61 tagatcaata agaatggaga gatatttact gttatgaata aactttcata tgcgacgcat
      121 gaatataaat aaaataatat cgagatattt tatatactga tttttttatt attcgcaaac
      181 tcagtaataa gctttgctaa tatatttcag gaaaaccgct taccctgaat attcagggta
      241 agcgtgagag ttaagaatga attacagcgg ttgggtttgc gcttcaacca cggccagcgc
      301 caccatattg acgatacgac gcaccgatgc gatcggcgtt aacacgtgaa ccggtttcgc
      361 cacgcccatc agcaccggac cgacagtcac accttccgag ctggaaacgc gcagtaagtt
      421 gtaactaatg cgggcagctt ccatgttcgg catcaccaga atattggcag aacctttcaa
      481 agggctgtcc ggcatacggt cgttgcgaat cgcttccacc agcgctgcat cgccgtgcat
      541 ttcaccatca atcatcagat ctggcgcacg atccctgacc agttccagcg cctgacgcat
      601 tttgctcgac gacgggcagt cagaagaacc aaagttggag tgcgacaaca gagcaacgcg
      661 cggctcaata ccaaaacgac ggacagtttc tgccgccatc aaggtgatct ccgccagctc
      721 ttctgcgtct ggttcgtcat tgacgtaggt atcggcaata aaggtgttac cactcggcag
      781 cagcagcgcg ttcattgcac ctgcggtgtg aacgccatcg cgataaccaa agacattttt
      841 caccacgcta aaatgttcat gataatcgcc caccgtaccg cagatcattg catcggcttc
      901 cccacgctga accatgatcg cgccgatcac tgtcgggtta ctgatcagcg cacgctgcgc
      961 ctgttcctga gtgacaccgc gacgcttcat gatctggaag tattcggtcc agtactcttt
     1021 aaagcgcgga tcggattcgt tattgacgat ttcaaaatca acgcccgctt tgatctgcaa
     1081 gcccagtttc tgaatgcgca tttcgatcac gttcggacga ccgataagga tcggtttcgc
     1141 cagtcccagc gttaccagtt cctgagtcgc atgcagaacg cgcgcctctt ccccttccgg
     1201 cagaactacg cgcttcggcg ctttgcgagc ctgggagaaa atcggcttca taaacaggtt
     1261 ggttttgtaa acgaactcag tcagcttgtc gatgtagacg tcgaaatcag caatcggacg
     1321 cgtcgccacg cccgactcca tcgccgcttt tgcgaccgca ggagcgatct taacgatcaa
     1381 gcgcggatca aacggttttg gaatgatgta ttccggacca aagctcagat cctgaacgcc
     1441 atacgctgaa gccaccactt cactctgttc tgcatgggcc agttctgcaa tcgcgcgtac
     1501 cgccgccagt ttcatctctt cgttgatggc ggttgcgcca acgtccagcg cgccacggaa
     1561 gatgaacggg aagcaaagaa cgttgttcac ctggttcggg tagtcggaac ggccggtaca
     1621 gatgatggca tccggacgca cttctttcgc cagcggcggc agaatttccg gttccgggtt
     1681 cgccagcgcc aggatcattg gtgcccgagc cattttcttc accatttcct gggtcagcac
     1741 tttcgggccg gaacagccca ggaaaatatc cgcaccttca atcacatcat cgagggtacg
     1801 tttgccgtca tccaccaccg catatgcggc tttggtttcc gccatatttg gctcacggcc
     1861 ctgatagata acgccttttg aatcgcaaac cacgatgtta tgtttttgca gccccagcgc
     1921 taccagcagg ttcatacagg cgattgctgc ggcacccgcg ccggaaacca ccatccgcac
     1981 gtcggagatg tttttctcca ccacgcgcaa gccgttgagg atggcggcag tgctgatgat
     2041 tgccgtgccg tgctgatcgt cgtggaatac cggaatattc atccgctcgc gcagtttctg
     2101 ttcaatatag aaacattctg gcgctttaat gtcttcgagg ttgatgccgc cgaaggtcgg
     2161 ttcgagcgcg gcgacgactt caataaattt gtccgggtcg agttcatcaa cttcaatgtc
     2221 aaatacatca atcccagcga atttcttaaa tagaacgccc ttgccttcca tcaccggttt
     2281 gcctgccagc gcgccaatgt tgcctaaccc cagcaccgcc gtaccgttag agatcaccgc
     2341 aaccaggtta ccgcgcgcgg tatatttgta ggcttttagc gggtcttttt cgatttcaag
     2401 acaaggtgcg gcaacgcctg gtgagtaggc cagcgccaga tcgcgctgcg ttgccagagg
     2461 cttggttgga gaaacctgga ttttccctgg aactggaaat tcatggaaat caagtgcact
     2521 ttgttttaac tggtcatcca tttggttgtt cctttcacgt aacgttcaca aataaagtgt
     2581 gtgggcaaca gcccctgccc acaacgtggc gcacattatt accctgccgg agtctacaga
     2641 ctttgagcaa gtccaaactc tcaccattaa tataatgttt tggtaataat cctataacac
     2701 tgatgttacc tgcttaatcc agcaatacca tgcctgtctg ctatgctttt ttgatgcgtt
     2761 tagcgaaatt tctcagaagt gtgaattaac gcactcatct aacactttac ttttcaagga
     2821 gtatttccta tgaacgagtt agacggcatc aaacagttca ccactgtcgt ggcagacagc
     2881 tgcgatattg agtccattcg ccattatcat ccccaggatg ccaccactaa tccttcgctg
     2941 ttactcaagg ctgccggatt atcacaatat gagcatttaa tagacgatgc tatcgcctgg
     3001 ggtaaaaaaa atggcaagac ccaggaacaa caggtggtcg cagcgtgtga caaactggcg
     3061 gtcaatttcg gtgctgaaat tctcaaaatc gtacccggtt gcgtgtcaac agaagttgat
     3121 gcacgcctct cttttgataa agaaaagagt attgagaagg cgcgccatct ggtggacttg
     3181 tatcagcaac aaggcgttga gaaatcacgc attttgatca aactggcttc gacctgggaa
     3241 ggaattcgcg cggccgaaga gctggaaaaa gaaggtatta actgcaacct gacgctgctg
     3301 ttttctttta cccaggcgcg ggcctgtgcg gaagcaggcg tttttctgat ttcgccgttt
     3361 gtagggcgta tttatgactg gtatcaggca cgcaagccga tggacccgta tgtggtggaa
     3421 gaagatccgg gcgttaaatc ggtgcgcaat atctacgact actataagca acaccactat
     3481 gaaaccattg tgatgggcgc gagcttccgt cgcaccgaac aaatcctcgc cttaaccggc
     3541 tgcgatcgac tgactatcgc accgaatttg ctgaaggagc tgcaggaaaa agtttctcca
     3601 gtggtacgta aattaatccc gccttctcag acgttcccac gcccagcacc catgagcgaa
     3661 gcggagttcc gttgggagca caatcaggat gcgatggcgg tagaaaaact gtctgaaggc
     3721 attcgtctgt tcgctgttga tcaacgcaaa ctggaagatc ttcttgccgc caaactataa
     3781 accagccacg gagtgttata tgtcccgaaa agaccttgcc aatgcgattc gcgcactcag
     3841 tatggatgcg gtacaaaaag ccaattctgg tcatcccggc gcgccgatgg gcatggctga
     3901 tattgccgaa gtgctgtgga acgattttct taaacataat cctaccgacc caacctggta
     3961 tgatcgcgac cgctttattc tttccaacgg tcacgcgtcg atgctgctct acagtttgct
     4021 acatctgacc ggttacgacc tgccgctgga agaactgaag aacttccgtc agttgcattc
     4081 gaaaacccca ggccacccgg agattggcta tacgccaggc gttgaaacca ccaccggccc
     4141 gcttggacaa ggtttggcga acgccgtcgg gctggcgata gcagagcgta cactggcggc
     4201 gcagtttaac cagccagacc atgagatcgt cgatcacttc acctatgtgt ttatgggcga
     4261 cggctgcctg atggaaggta tttcccacga agtctgttcg ctggcaggca cgctgggact
     4321 aggcaagctg attggttttt acgatcacaa cggtatttcc atcgacggtg aaacagaagg
     4381 ctggtttacc gacgatacgg caaaacgttt tgaagcctat cactggcatg tgatccatga
     4441 aatcgacggt cacgatccgc aggcggtgaa ggaagcgatc cttgaagcgc aaagcgtgaa
     4501 agataagccg tcgctgatta tctgccgtac ggtgattggc tttggttcgc cgaataaagc
     4561 aggtaaggaa gaggcgcacg gcgcaccact gggggaagaa gaagtggcgc tggcacggca
     4621 aaaactgggc tggcaccatc cgccatttga gatccctaaa gagatttatc acgcctggga
     4681 tgcccgtgaa aaaggcgaaa aagcgcagca gagctggaat gagaagtttg ccgcctataa
     4741 aaaggctcat ccgcaactgg cagaagagtt tccccgacgg atgagcggtg gtttaccgaa
     4801 ggactgggag aaaacgactc agaaatatat caatgagtta caggcaaatc cggcgaaaat
     4861 cgctacccgt aaggcttcgc aaaatacgct taacgcttac gggccgatgc tgcctgagtt
     4921 gctcggcggt tcggcggatc tggctcccag caacctgacc atctggaaag gttctgtttc
     4981 gctgaaggaa gatccggcgg gcaactacat tcactacggg gtgcgtgaat ttggcatgac
     5041 cgctatcgcc aacggcatcg cgcaccacgg cggctttgtg ccgtataccg cgacgttcct
     5101 gatgtttgtt gaatacgccc gtaacgccgc gcggatggcg gcactgatga aagcgcggca
     5161 gattatggtt tatacccacg actcaattgg cctgggcgaa gatggtccga cgcaccaggc
     5221 tgttgagcaa ctggccagcc tgcgcttaac gccaaatttc agcacctggc gaccgtgcga
     5281 tcagatggaa gcggcggtgg gctggaagct ggcggttgag cgccacaacg gaccgacggc
     5341 actgatcctc tcaaggcaga atctggccca ggtggaacgt acgccggatc aggttaaaga
     5401 gattgctcgt ggcggttatg tgctgaaaga cagcggcggt aagccagata ttattctgat
     5461 tgccaccggt tcagagatgg aaattaccct gcaagcggca gagaaattag caggagaagg
     5521 tcgcaatgta cgcgtagttt ccctgccctc gaccgatatt ttcgacgccc aggatgagga
     5581 atatcgggag tcggtgttgc cttctaacgt tgcggctcgc gtggcggtgg aagcaggtat
     5641 tgccgattac tggtacaagt atgttggtct gaaaggggca attgtcggga tgacgggtta
     5701 cggggaatct gctccggcgg ataagctgtt cccgttcttt ggctttaccg ccgagaatat
     5761 tgtggcaaaa gcgcataagg tgctgggagt gaaaggtgcc tgatggtgat cgccggatgc
     5821 tgtatgttcc cctcacccta accctctccc cagaagggcg aggggacggt ccgtgcgctt
     5881 tttagggccg catctgcgat tttgtttaac gggtgatcca caacgtgggc ctggcgtctg
     5941 gcccatgcca gttatcgcag gtaggttctg cggcgtaacg caccaggcga aaacgctgac
     6001 cgtcaaagcg ccagcgcgcc tgaatgccac aatcgcttaa tccgcgccct ttcgctaagg
     6061 tcaccagttc acgcgatttc tcatcaaatg ttgcgttcat cagttccagt tcattcgtct
     6121 cctggccgtt gttgaacggc aaacgcaacc gaaccgggcg cgaggctagc ggctttttac
     6181 gcgacacaat ccatgccaaa tcaatggtgt tataggcccc tgcctcacag ctaatcatca
     6241 tcagtgcttt atcatcggtc agcgcagtga cattcacctc acgacgtaac ggatcaagcg
     6301 agcagcgcag accattcatc cgccagtttc cataatccag caaatcgtta cgttcttcga
     6361 gtgagagtgg cgtcggcgtt gggttaacca ccgcgacctc tttcagcgca ggcgcgggcg
     6421 gtacgctgag aggcggttcg tcccctttct tgatccacgc ggtttcactg ccaacgcgct
     6481 tttgctgagc atcaataaac aacaacgccg cttttaagcc gctcagagaa atggtctgat
     6541 tgccgtcgcg taaggtgatc gccttccctt cctgaattat ctgcaaaaac gcggtgatgg
     6601 ttgccgtatc atcggttact aataaccatg gtgaaatccg ccacttgtca ccacttaatg
     6661 ccaacggctc accatctaat agcagccgtg gcgctatctc cccttcagac gcatccggcg
     6721 acttcaaccc accgcgctca atacgtaaaa cggcatcggt atgcgcccca gcgctgcggc
     6781 tcagggtcat caccagtcca ttatgatcgc ccgtattgcg cgccacacaa aaattttgat
     6841 tattacaggt gacctgccag tcggaaaacg cccgttgcgc tggtgccgcc cacaccaaag
     6901 acgtcggcaa caaagcgaaa aaaaagagaa ggaaaatgcg atagcgcatg gacggtacga
     6961 ccccagaagt tcaaacaaac agcacaagtc gtatcttcgt tgcgcaggta gcgcggctca
     7021 atcggatttg tcggattgag ccaataataa acaacgaaat attattgcta tttttcagtc
     7081 cattaaatgt gacatttgca aatagttaag caataacacc gtcttaccgt cacgtatctc
     7141 gccggttttg atcatctcca gcgcctggct gaacggcagc tcgagcactt caatatcttc
     7201 atcttcaaca ccgccaccgg cgttagcgcg ctgattgtca ctgtattcgg cgataaaaaa
     7261 gtggattagc tcagtcacac cgccaggcga catatacagt tcaaataatt tgcgcacttc
     7321 gcccacttca tagcccgtct cttcaatcgc ttctttacga atgcacactt ccggttcgtc
     7381 gttatccagc agcccggcgc aggtttcaat cagctgcccg ctttcattgc cattaaccca
     7441 ggtagcgaca cggaactgac gaatcagaac cacgctcttt ttcttcgcgt tgtacaggag
     7501 gatcgtcgca ccattaccgc gatcgtaaac ttcacgttta tggcggataa cttcgccatc
     7561 tttgcgcgtg agatcgtaag taatgttgtg cagggtgaaa tagttatcgg agagaatttt
     7621 gtctttaatg agggtgattt gttgcgtcat accgactcca cagcgcgaaa tgaacaatta
     7681 tattacgctg tgaagtcggg tttgtctgcg caggctatca gtgagatttg actgatttta
     7741 cccccagcca gtcaataatc ccctgtgccg catgacgtcc ttctgccatt gcggtaacca
     7801 ccagatccgc accacgcacg gcgtcgccac cggcgaagat tttcggattg gtggtctggt
     7861 aacggtactg gctttccaca tccgcgatga tgcggcccca tttgtctacc gttacaccgt
     7921 gcgactccag ccacggcatc ccgtgtggat tgaagccaaa cgccataatc accgcatcgg
     7981 ctggcatgac aaattcactg ccctcaatca gtacaggtcg ccgacgcccc tgggcatccg
     8041 gctcgccaag acgcgtgcgc aggaaacgaa ttccgcaaac gtgaccttgc tcattcagct
     8101 caagcgccac cggctggacg ttaaattcga agttggcccc ctcttcgcgg gcgttcttca
     8161 cctctttctt cgagcctggc atgttagctt catcacgacg ataagcgcag gtgacgttac
     8221 tcgcgccgtg gcgcagcgca gtacgcacac agtccatcgc ggtgtcgccg ccgcccagta
     8281 ccacgacgtt aagtccggcg gtattgataa acggctcttc cggtagctct tcgagcccca
     8341 tcacctgttt agtgttggca atgaggaacg gcagcgcgtc ataaacgccc ggcgcatctt
     8401 cattgggtaa acccgctttc atggaacggt aagtgcctac gccaacgaag accgcgtcgt
     8461 attgttccaa aagcaaatcc aaagagacat ctttacccac ttcacaattg agttcgaagt
     8521 gaatccccat cgcgctgaag atttcccgac ggcgtgccag cagggattta tccagtttga
     8581 aagaaggaat gccgaaagtg agcaagccac cgatttctgg atggcgatcg tacaccgtca
     8641 ccgccacgcc attgcgggtc agaacatccg cacaggccag ccctgccgga cctgccccga
     8701 taatcgccac ccgcttgtcc actttggtga catgacttaa gtcaggacgc caacctttcg
     8761 ccaacgcctg atctgaaata tagcgttcaa tgttgccgat agttaccgcg ccgtgctcat
     8821 cgcgaatagt acaggcacct tcacacaaac ggtcttgcgg acaaacgcgt ccggtaattt
     8881 ccggcagggt gttggtctgg tgagaaagct cgacggcggc gtcgatgttt ccggctttca
     8941 ccagttcaat ccactgcgga atatggttat gcagcgggca ggtccattca caaacgctgt
     9001 gctcgccgaa cttaaggcag cgcgaggctt cccgttgtgc ctggtcggcg cgaaatggca
     9061 gataaatttc atcaaaaccg gttttgcgcg cttcaatcgc cagtttatcc ggctcgccac
     9121 gcgcgggcgt tgcctgcatt tgttcgactt tactcattac cggcatttct tgtgcagcgg
     9181 tactggcatg ccacggttga tgctcctgac gtgcggtgcg caagcggcgg gatttcgcca
     9241 tgccggagag tgcgacgtca gtgaccagtt gcagcgcgtc cgccgggcaa ttctcgacac
     9301 aggcaggacc gttttcgcgc cctgcacaaa ggtcgcattt atgcgccgtg gcttttactt
     9361 ttcctgccgc gacgggtgtc aggacgattt gcatcgtacc aaacggacag gccaccacgc
     9421 aggatttaca gccaatacac ttttgctgat tgacctgaat gctgtcatca acgtggctga
     9481 ttgcgccatt agggcagcta cgggcgcagg gcgcatcttc acaatggtga caggtcactg
     9541 cactacgttg ctgctgatgt ttgataaccg taattcgggg atgaaaatgg tgttggctca
     9601 ggacatgttg ctcatcattg tgagccatga cacaggcgat ttcacaagca tgacaaccca
     9661 gacattgctg actgttggcc ataataaaac gattcataac gaccttcttt tttggttgta
     9721 aaaaccttat tctttatatg agtgttgtta ttacccgact tacaggggaa ccggcaatgt
     9781 tcatgtgccc agaataagta actatttcgc tataaactgt ggcagatcaa ataatcccat
     9841 cactgactaa attgcgtttc agcgaactgg aacattaatg attttttgtg gagaagacgc
     9901 gtgtgattgt taaacgaccc gtctcggcca gtctggcccg ggcctttttt tacattgtgc
     9961 tgctgtcgat tctttccacg ggtatcgctc tgctaactct ggcgagcagt ttgcgcgacg
    10021 ctgaggctat caatattgcc ggatcgctgc gtatgcagag ttaccgcctg ggctacgact
    10081 tgcaaagtgg cagtccacaa ctcaatgcac atcgccagtt gtttcagcag gcactgcatt
    10141 caccggtatt aaccaacctc aacgtctggt atgtgccaga agcagtaaaa actcgctatg
    10201 cgcatctgaa tgccaactgg ctggagatga ataatcggct cagcaagggc gatttgccgt
    10261 ggtatcaggc caatattaat aattatgtta atcagataga cctgttcgtg ctggctttac
    10321 agcactacgc tgaacgcaaa atgctgctgg tggtggcgat ttccctggct ggcggtatcg
    10381 gtattttcac gctggtcttt tttactttgc gccgcatacg ccatcaggtg gttgccccgc
    10441 tgaatcagct ggttaccgcc agtcagcgta ttgaacacgg gcagttcgac tcgccgccgc
    10501 tggataccag tctgccgaat gagcttggtc tgcttgcaaa aacctttaac caaatgtcga
    10561 gcgagctgca taaattgtac ctttcgctgg aggcgtcagt agaagaaaag acccgcgatc
    10621 tccacgagac caagcgtcgt ctggaggtgt tgtatcagtg cagtgttccc aggcgctgaa
    10681 caccagccag attgatgtgc attgtttccg ccatattttg cagattgttc gcgacaatga
    10741 agcagctgaa tatctggagc tgaacgttgg ggataactgg cggattagcg aagggaagcc
    10801 aaaccctgag ctgccaatgc agattttacc ggtgacaatg caagagacgg tttacggcga
    10861 actgcactgg caaaatagtc acgtttcatc atcagaaccg ctgcttaaca gtgtttcatc
    10921 gatgctggga cgcggtttgt actttaatca ggcgcagaag cattttcagc agttattgtt
    10981 gatggaagaa cgtgcgacca tcgcccgcga attgcacgac tcgctggctc aggtgctttc
    11041 ttacttacgt atccagttga cgttactgaa gcgttcgata ccggaagata acgccaccgc
    11101 acaaagtatc atggccgatt ttttccaggc gttgaatgat gcttatcggc agttacgcga
    11161 gctgttgact actttccgcc tgacgctgca gcaggcggat ctcccctccg cgttgaggga
    11221 aatgctggat acgttacaaa atcaaaccag cgctaaactg accctcgact gccgtctgcc
    11281 aaccctggca ctggatgcgc aaatgcaggt gcatttgttg caaattattc gcgaagcggt
    11341 gctgaatgcg atgaagcacg ccaacgccag cgaaatcgcc gtcagttgcg tcaccgcgcc
    11401 ggacggcaat cacacggttt atatccgtga taacgggatt ggtatcggtg aaccgaaaga
    11461 acccgaaggt cattatggac tgaatatcat gcgcgaacgc gcggaacggc tgggcgggac
    11521 gctgactttt tcacaacctt ccggcggcgg cacgttagtg agtattagct ttcgctccgc
    11581 agagggtgag gggaagggga acacgccggc tgaggtgtaa ataattaccc tcacaccggc
    11641 cctctccctt taaaggcgag agggaaaaac gtgcccatat tgacctgtaa gcgagtgtgt
    11701 cagcaatctc atgataattt acattaactc ctttttttct ccacgattgg ctcgtacctt
    11761 gccgctacag tgaagcaagt caagcctaca acaatacgca gaaacacgag gtcctctttt
    11821 aatggcgaat ttctttattg atcgccccat ttttgcctgg gtgctggcaa tcctgttgtg
    11881 tctgacaggt accctggcga ttttttcatt gcccgttgaa caataccccg atctcgcgcc
    11941 accgaatgtg cgagtgaccg ctaactatcc cggcgcatcg gcccagacgc tggaaaacac
    12001 cgtgacccag gttatcgagc aaaatatgac cggcctcgat aatctgatgt atatgtcatc
    12061 tcagagcagt ggcaccggtc aggcatctgt cactttaagt tttaaagcag gcaccgatcc
    12121 ggacgaagcc gtgcagcaag tacaaaacca gctgcaatca gccatgcgaa agttaccgca
    12181 ggcggtgcaa aatcagggcg tgacggtgcg taaaaccggc gataccaaca ttctgaccat
    12241 tgccttcgtc tctaccgatg gctcgatgga taaacaggat attgctgatt atgttgccag
    12301 taatattcag gacccgttaa gccgcgtgaa tggcgtcggg gatatcgatg cctatggttc
    12361 gcaatattcc atgcgtatct ggctggaccc ggcgaaactc aacagtttcc agatgacggc
    12421 taaagatgtc actgatgcca ttgagtcaca gaacgcgcag attgcggttg ggcaacttgg
    12481 tggtacacct tccgtcgata agcaggcgct caacgccacc attaacgccc agtcactgct
    12541 gcaaacacca gaacagttcc gcgatatcac cttgcgggtg aatcaggacg gctcagaggt
    12601 aaggctgggc gatgtcgcca ccgtcgaaat gggggcggag aaatacgatt atcttagccg
    12661 cttcaatggt aagccagcct ccgggttggg ggtaaaactg gcctccagcg caaacgaaat
    12721 ggcgacagcg gagctggtgc tcaatcgtct cgacgagctg gcgcagtatt tcccgcacgg
    12781 actggaatac aaggtggcgt atgaaaccac ctcgtttgtt aaagcctcca ttgaagacgt
    12841 ggtgaaaacg ctgctggaag ctatcgctct ggttttcctc gttatgtatc tgttcctgca
    12901 aaacttccgc gccacgctga tacccactat cgccgtgccg gtggtgttga tgggaacctt
    12961 ctccgtactt tacgccttcg gttacagcgt caacacctta accatgttcg cgatggtgct
    13021 ggcgatcggt ctgctggtgg atgacgccat cgtggtggtg gaaaacgtcg aacgtattat
    13081 gagtgaggaa ggactcactc ctcgcgaagc cacacgtaaa tcgatggggc agatccaggg
    13141 ggcactggtc gggattgcga tggtgctttc ggcggtattt gtaccaatgg ccttcttcgg
    13201 cggcaccacc ggtgccatct atcgccagtt ctctattacc attgttgcgg cgatggtgct
    13261 gtcagtactg gtagcgatgc tcctcactcc ggctctgtgt gccacgctac ttaagccact
    13321 gaaaaaaggt gagcatcacg ggcaaaaagg tttttttgcc tggtttaacc agatgtttaa
    13381 ccgcaacgcc gaacgctacg aaaaaggggt ggcgaaaatt ctccaccgta gcctgcgctg
    13441 gattgtgatt tatgtcctgc tgcttggcgg catggtgttc ctgttcctgc gtttgccgac
    13501 gtcgttctta ccgctggaag accggggcat gtttactacc tcggtacaat tgcccagcgg
    13561 ttcaacccaa caacagaccc tgaaagtcgt tgagcaaatc gagaaatatt acttcaccca
    13621 tgaaaaagac aacatcatgt cggtgtttgc caccgttggt tctggccctg ggggtaacgg
    13681 gcaaaacgtg gcgagaatgt ttatccgcct gaaagactgg agcgaacgcg acagtaagac
    13741 cggcacctcg tttgccatta tcgagcgtgc aacgaaggcg tttaaccaaa ttaaagaagc
    13801 tcgcgttatc gccagcagcc caccagcaat tagcggtctt ggtagttctg caggttttga
    13861 tatggagttg caggaccacg ctggagcggg tcacgatgcg ctgatggcag cacgtaatca
    13921 gttgctggcg ctggcggcgg aaaacccgga gctaacccgt gtgcgccata acggcctcga
    13981 tgacagtccg cagttgcaga ttgatatcga ccagcgtaaa gctcaggcgc tgggcgttga
    14041 tatcgacgat attaacgaca cactgcaaac cgcctggggt tcgagctatg tgaatgactt
    14101 tatggatcgc ggtcgcgtga agaaagtcta tgtgcaggca gctgcgccgt atcgcatgct
    14161 gccagatgac atcaatctct ggtatgtccg aaataaagat ggcggcatgg tgcccttctc
    14221 tgctttcgcg acctcacgct gggaaacagg ctcgccgcgt ctggaacgct ataacggtta
    14281 ttctgcggtt gagattgttg gggaagccgc accgggggtc agtaccggta cggcgatgga
    14341 tattatggaa tcgttagtga agcagctgcc aaacggcttt ggtctggagt ggacggcgat
    14401 gtcgtatcag gagcggcttt ccggcgcgca ggctccggcg ctgtacgcca tttccttgct
    14461 ggtggtattc ctgtgtctgg cggcattgta tgaaagctgg tcggtaccgt tctcggtaat
    14521 gctggtcgtg ccgctggggg taatcggcgc gctgctggca acctggatgc gcgggctgga
    14581 aaacgacgtt tacttccagg tgggcctgtt aacggtcatt ggtttatcgg cgaaaaacgc
    14641 catcctgatc gtcgagtttg ctaacgagat gaaccaaaaa ggccatgacc tgtttgaagc
    14701 gacgctccac gcctgccgtc agcgtttacg cccgattctg atgacctcgc tggcatttat
    14761 cttcggcgta ttgccaatgg caaccagcac gggtgccggt tccggtggtc agcatgcggt
    14821 gggtactggc gtaatgggcg ggatgatttc agctactatt ctggctattt acttcgtgcc
    14881 gctgttcttt gtgctggtgc gccgccgctt cccgctgaag ccgcgcccgg aataagcaat
    14941 aaaaaaggcg acatgccaat gtgtcgcctt tttcaacttt ccgattaaga acctgctcag
    15001 cgggttcttg ctgtttatac tttgtctcag gaattactta cgaagcataa cttcgataaa
    15061 gtctttccag ttccccagtt cacgttcaat cataacaacc tctcttataa ttatgggtat
    15121 tctacggaaa caatataccg tggtgaagct aatttactcg attgctgcga tgactacctc
    15181 cgggggacaa accttatgta aatactatgg tcctgcggtg ataatttgta tgtgatacac
    15241 agcaacattt cgagatattc atacggcatc taatactgat ttaattctgg ttaaaataca
    15301 gacagataac aagatgaata ttcttaatgt ttacgttaaa aatgtttaat attatttaat
    15361 agttgttaat ttgaatactt cgataatgtt atattttctg ataatcattt gcaggcaaaa
    15421 tgttttcacc cttaaatgag tatttattct cataaatcga aaaaggattc attatggtta
    15481 cactttacgg catcaaaaat tgtgacacca ttaaaaaggc tcgccgttgg ctggaagcca
    15541 ataacatcga ctatcgtttt catgattacc gcgtcgatgg gctggacagc gaattattga
    15601 acggttttat caacgaatta ggctgggaag cgttactcaa cacccgtggt acaacctggc
    15661 gtaaactgga cgaaactacc cgcaataaaa tcaccgatgc ggcctctgcg gcggcattaa
    15721 tgactgaaat gcctgcaatt atcaaacgtc cattgctctg cgcgcctggt aagcctatgc
    15781 tgctgggttt cagtgattcc agttatcagc aatttttcca tgaggtgtag tctatgtcgt
    15841 gcccggttat tgagctgaca caacagctta ttcgccgccc ttccctgagt cctgatgacg
    15901 caggatgtca ggctttgttg attgaacgtt tgcaggcgat cggttttacc gttgaacgca
    15961 tggattttgc cgatacgcag aatttttggg catggcgtgg gcaaggtgaa acgttagcct
    16021 ttgccgggca taccgacgtg gtgccgcctg gcgacgccga tcgttggatc aatccgccgt
    16081 ttgaacccac cattcgtgac ggcatgttat tcgggcgcgg tgcggcagat atgaaaggct
    16141 cgctggcggc gatggtggta gcagcagaac gttttgtcgc acaacatccc aaccatacgg
    16201 ggcgactggc atttctgatc acttctgatg aagaagccag tgcccacaac ggtacggtaa
    16261 aagtcgtcga ggcgttaatg gcacgtaatg agcgtctcga ttactgcctg gtcggcgaac
    16321 cgtcgagtat cgaagtggta ggtgatgtgg tgaaaaatgg tcgtcgcgga tcgttaacct
    16381 gcaacctcac cattcatggc gttcaggggc atgttgccta cccacatctg gctgacaatc
    16441 cggtacatcg cgcagcacct ttccttaatg aattagtggc tattgagtgg gatcagggca
    16501 atgaattctt cccggcgacc agtatgcaga ttgccaatat tcaggcggga acgggcagta
    16561 acaatgttat tccgggtgaa ctgtttgtgc agtttaactt ccgcttcagc accgaactga
    16621 ctgatgagat gatcaaagcg caggtacttg ccctgcttga aaaacatcaa ctgcgctata
    16681 cggtggattg gtggctttcc gggcagccat ttttgaccgc gcgcggtaaa ctggtggatg
    16741 cggtcgttaa cgcggttgag cactataatg aaattaaacc gcagctactg accacaggcg
    16801 gaacgtccga cgggcgcttt attgctcgca tgggggcgca ggtggtggaa ctcgggccgg
    16861 tcaatgccac tattcataaa attaatgaat gtgtgaacgc tgccgacctg cagctacttg
    16921 cccgtatgta tcaacgtatc atggaacagc tcgtcgcgtg atgagtggtt ctgcaagagg
    16981 aaataagcat ggactggctg gctaaatatt ggtggattct ggtgattgtc tttttggtag
    17041 gcgtcctgct gaacgtgatt aaagatctca agcgcgtcga ccataagaaa tttctcgcca
    17101 acaagccgga gcttcccccg catcgtgatt tcaacgataa gtgggacgat gacgacgact
    17161 ggccgaaaaa ggatcaaccg aagaagtaag ctgagtgctc aaccgtaggc cggataaggc
    17221 gttaacgccg catccggcaa tggtgaacga tccctgatgc gacgctcacg cgtcttatca
    17281 ggccaggtaa tctgtctttt acatcatttc aatcacatca tcatcgccag gcttaccgcc
    17341 acttagcgct tggtcgaaat aatgtttcgg aatggtataa cgcaagtgat ccagggcaaa
    17401 ttgcatactg cgattgtcga tcgcatggcc taaatcctcc acgatatcca gcgtcacgtc
    17461 accaccggca ctgattaacg cttcctgcgc cgcaactgca tgcgccagat cgataaccgg
    17521 atcttcacca ccatgaatca agtgaattgt cgtggctgta gacgcggtct ccggcaggct
    17581 cgcatagcgt ccgttaaggg caatgacgag cgaagcaagg cccggttcgg ctttaatgct
    17641 ctccagcgcc ataatcgcgc cttgtgagaa accaattagc gccgtggcgt tagcccctac
    17701 tccgctctgt ttctgccagt agcgtaccgt ttcaataaac gtcggcatga tggcatccac
    17761 acgcgcctgg cgattatctt ccgtaatacc ctgcactgaa aaccactgac gccccgccgg
    17821 attaccgctc ggctccgcgc caccgacact caccaccagc gcatcaggaa acagcggtgc
    17881 aaaccagctg cctatttccc ccattgccac cgggttatcc ccgacaccgt ggaaaagcag
    17941 taacaactgt tgtgcaggtt tatccgggct ttgaacaaca aaatggtcat gtttcatggc
    18001 gaactcctta actgatgtca ttaattttac gccgctgact cacaatgacc atgggagaaa
    18061 attgaaggag tcagtgaaaa aattgccatt gcgttatgcg atcgcgcaga cgctcagtgc
    18121 gaacctcatt tagtgcgaac agcgcctgcg cggcctcttc ccgctggcga accagtaaca
    18181 tcttgcggcc tgaaagttta agtgtggtac ataactgcgc gtcactggcg tttttctgta
    18241 aacgcccacg cagcgccggt aatgccagtt cactggtttg tagcagacgc aataagcaac
    18301 ctaacgatgt taatagcgga cgatgagcga aagcaaaacc ggccagttca agccagtcgt
    18361 cgtcagaaag gatggcatcg tttagtggat caacaggaag catttcgcca ttccactgcg
    18421 cgagagcttg cgcatcgcga cgtaaacggt aatgctcacg ttcagccagc tgtttacccg
    18481 catcactcat cggtaacagc gccatcgccg tatagcaacc gctgctggct tcccgatgat
    18541 tacccatccg caccagcaca aaaccgcagc gttgccagaa acgccataac tccccagtgt
    18601 aaccaaaact caccgaaaga tagtcgaggt catgcgtata ttgcaaagct ccagcaataa
    18661 gttgtcgccc tgtgccttcc cgctgacgag ccggatgaac tgctatccgg ctaacccgcc
    18721 ttccgcgcaa tgtcgccgcc agtggattgc tgccgtgcgc cgccagcgac tgggccacca
    18781 gattaccccg cgggcgacga aaacctgccc ataccgcctg actgagttct tgagataatc
    18841 cgccctcatc caccagccac aacgccccgg caatctcgtt ttcgccagcc gcctgtaaaa
    18901 aatgttgccc tggtgcatcc atcatccggc gtaaatccag cggcgaggtc cggtagtgcg
    18961 caccagacaa taactgataa acctttaacg gcgtttctgg ctcgcttcgc cataacgttt
    19021 gttcaaatgc ggaaatgacg atattgcctt gtggtgtatg ggtgaagttt tcatcgttaa
    19081 aaaccagtgc ctcactaacc attttttcca gcgggcatcc ctgtgcccag cggatcggct
    19141 gttgcagttc aaaacggtgt aaatgcggaa agcgagcgca aaatttcagc aaaaaaccac
    19201 gtccggtacc ttcgtagccc tgcaccgtag tggttaacaa cgttcgagga aaacgcgata
    19261 ccagttgatg caacaacggc gcaggtatgg ctgcggcttc gtcgaccacc agccagtcgg
    19321 cttgctcatc gctggctaac aaggcatccg gtgcaataaa gcgaaacttc tcgcccgcaa
    19381 attgtgccag tacatccgtt gccgcttttg cgggcgcggt gacaatcgca ctgcccgcaa
    19441 tacgagaaat gagttgccct gccaacgccg acttaccgcg cccacgcgca gccgttaccg
    19501 ctgccacgcc cggcggcatg gtcagtagct gctgtaagag ttgctgttgt tctggttgtg
    19561 gtgcgccggt cgcggggtgc cagtcagtac ggggagtaaa atgcgccaac gagaacggct
    19621 ggttttgccg ccagaggata gcgtcgttat ccgccgtaag tacgcgtttg agatgctgga
    19681 caaaatgcgg cgtcgcaata gggtcagggc aatcactcca gcgcagcgag tcggcatcag
    19741 gttggttttc ccactcttcc catacaggga gtaacaaaac cagccagctt cccgctttca
    19801 acgttccgct aagtgctgca aaggcagcgg catcaaagcc gtggcgggcg tcgaataccg
    19861 catgccggaa ctcgcgccca agtaaagttt gtagcgccga gggtgaacag tggttttcag
    19921 catctagctg cggcgaaatc cacagccagt cgccaggtaa ggcatcacgc aacttaagag
    19981 catgatcaaa acaccaaccc tcttccccgc tcaacaccag caagcggcag atcccttcac
    20041 gtttcatttg cgctgttaat gtgtaaagcg cagtcagttc agccatccct gccccggaaa
    20101 ttaaatgctt ttaccaaaag tattgcattg tgccggatcg ccgctgtcga aaccacgttt
    20161 aaaccagctg tagcgttgct gagaagtgcc atgtgtgaaa ctgtctggta ctactcgccc
    20221 ctgactttgc tgttgtaaac ggtcatcgcc gatggcctgc gccgcattca gcgcctcttc
    20281 cagatcgcca gtttccagaa cgccttgctg ctgcatacta tgcccccaga caccggcaaa
    20341 acagtcggcc tggagttcca tacgcacaga taagcggttt acttccgcct gcgtcgcgtt
    20401 ttgttgcagt tgacgaactt tcggctcgat gcctaacagt ttctgcacat gatgaccgac
    20461 ttcatgggcg ataacgtacc cctgggcaaa atcgccatcc gcgccaagtt tgtctttcat
    20521 gtcatcatag aaggagagat cgatataaac tgtgccatcc gccgggcaat agaacggccc
    20581 cataatggac tggcccgccc cgcagccggt gcgcgtcatt ccacggtaca tgaccagctt
    20641 cggttgctga taggtcttac ccatcttttc gaactgttgt ccccaggtgt cttccgtggt
    20701 tgccagaatc accgaggtga attttgcggc ttcgtcttca ttagggctaa ttgaccgcgt
    20761 tgattgttgt tgagaaaccg gctgcccggt catcaacccg gttaaatcaa cgccatagta
    20821 gcctgcaacc agcacgacta tcagtaaaat cagcccgcct ttaccgctcg gcagacggaa
    20881 accgggaccg cccattgacg gaccgccaga gctgttgcgc ctgtcttcaa cattgtcact
    20941 ttcacgtcgc ccttgccaac gcatagatac ctcaacaata tattcattat agaaatgatc
    21001 gtaggtggtt cagaggaaga ttaccacagg aaagcgggat gaaatggcgg aggaatgagc
    21061 gttcaatgca tatattccga gagcggttgt cttgccgtgc cagctgcacg gcaagatgat
    21121 gaacagaaaa atcagtccag ctgtacaccc aggcggcggg cgacggcttc ataggcttcg
    21181 atcaggccac cgaggctctg gcggaaacgg tctttgtcca ttttctccag tgtttctttg
    21241 tcccacaggc ggctgccgtc cggggagaac tcatcgccca gtaccacttc acctttgtac
    21301 agaccaaact ccagcttgaa gtcgaccaga atcagaccag catcatcgaa cagttttttc
    21361 agcacgtcgt tcgctttgta ggtcagctct ttcatacgcg ccaggttctc tttgctcacc
    21421 cagccaaagg tttcgcagta agattcgttg accatcggat cgtgcatggc atcgtttttc
    21481 aggaacagat cgaacagcgg cgggttcagc tcaatacctt cttcgattcc aagacgtttc
    21541 accagagagc cagcagcacg gttacgcacg acacactcaa ccggcaccat atccagcttt
    21601 ttcaccagac attcggtatc ggagagcaga cgctccattt gagtcgggat acccgcttca
    21661 gccagtttgc tcataatgaa gtagttgaac ttgttgttca ccataccttt gcgatcaaac
    21721 tgctcaatgc gcgcgccatc ccctgctgac gtatcattgc ggaattcgag caccaacagg
    21781 tccgggtttt ccgtgctgta tacggttttc gctttaccac gatacaactc agcttgcttt
    21841 tgcatcttta tcactcctgg gtgtgaatta acgttttaaa atcttttgct gtctggtgtg
    21901 ccggatgttt tgtcggatgc ggcgtgaacg ccttatccga actacgtccg acaaacaaat
    21961 ttcgtgcgaa ttaccgctaa aatcgcgttt tcctgtcgac gcacacgttt gcgtatcata
    22021 tcagaaaaaa gggccggatg attccagccc tgtattttta cttgctaaac gcagcctgga
    22081 agacagctac cagcgcgtcg ttctgactct gagtcagagt atgacctttc ggatcgatga
    22141 actgtaggct gctgcggtta tctaaatcgc caacctgcag tttatagtca ccggatgcca
    22201 ggcctggatc gctcgcgccc agttcctgcc agtcgctgtc agacagcggc ttataagtta
    22261 cggccatgtt gccctgcgaa cgggtgctgt cggtcacttt catgcccact ttttccagcg
    22321 ccgctggcag acgttgccaa accacattga acggcccgcg tacgaccagc attggtaaac
    22381 cggtgtcatc agctgcactt tgtacgtcca tagtggtgga ggcacgattt tgcgcagcgt
    22441 tcgcggcgtc agtggcagat ttatccagac cggcggaaat aacgttcatc atctccgtgc
    22501 tgtaacgctg catggaagcc gcgtctgcaa ccggtttgcc cgcctgttcc aggttcagca
    22561 gtttaaccgt aaccgcctgc tgataaccct gcggcttaac agagatttga taacgaccac
    22621 gatactgctc gtcttcgtcc agacggttcc attgtaccca atcggtggtc agtgtctgac
    22681 cagcatcatc acgttgggtg atggtgtagt ttttcgcctg cagcacgcta accacctgcg
    22741 gccacagagt attgccacga ccattttcca ccagcaatga agcggtatcg cccgtgaact
    22801 gggtacgcgc gccagaaacc agtgccaacg gctgggctgg tggacgaatg tccagcgcct
    22861 taccgacagc accactaccg ttggtcaccg ggattgcata atcaccggag gtcaccggca
    22921 aaatcattcc agccggggca tgaagctccg caagcggtgc cgcttccagg taggcttcat
    22981 caccactgac ctgacgctta tagcgtgagt cagaactaca ggcagcgagt aataaaacaa
    23041 gcgaaacacc cgcaaccttt gccaggcgcg acttttgaac agagtaagcc atcaaatctc
    23101 cctaaacttt acagcaaacc ggcatgctta agcgccgctc tgaccgtctc acgaccactg
    23161 tcggtgattg gtgtcattgg caggcgcagc gtatcggtcg ccacaagacc cagttcctta
    23221 catgcccatt tcaccgggat tggattgggt tcgacaaata gtttgttgtg taatggcatc
    23281 agacgctgat taataacgcg tgcctcggca aaatgccctt ctgctgccag tttgcacatc
    23341 tgggccatat cacgcgctgc gacgttagcc gtaacggaaa taactccatg accgcccaat
    23401 tgcatgaagt ccagcgcgct cgcatcatcg ccgctcagca gaacaaaatc atctgaaacc
    23461 agctctttga tctggtttac acgcgttaag ttccctgttg cctctttgat tccgataata
    23521 ttttttactt tcgccagacg gcccaccgtt tccgggagca gatcgcagcc agtacgggac
    23581 ggcacattat acagaatttg cggcaggtca gtatgctcag cgatggcttt gaaatgctga
    23641 tacaaacctt cttgcgacgg acgattgtag taaggggtta ccgtcaggca gccgacgata
    23701 ccactgtcat tgaagcgctg cgtcaggcta atggcttccg cagtagcgtt agcgccggtc
    23761 ccggcaatta ccggaatgcg cccatcagcc agatccagcg tcatcatcac cacatcagca
    23821 tgttcgtcat gatttaaggt agcggactcg ccagtggtgc caacagaaac gatcgccgaa
    23881 gtaccgctgg cgacatgata atcaatcagt tttttcaagc tagcccgaca gacattacct
    23941 ttttcatcca tcggagtaac aatcgcgaca atacttcccg tgaacatggg ccatcctctg
    24001 tgcaaacaag tgtctcaatg gtacgtttgg tatggcatta aaagcaagca gacagaaccg
    24061 ttctgattgt tgtatgcatg atttttttat gctttcctta agaacaactc accccttaaa
    24121 ggaataacca gtttgacact gtcatcgcaa cattatctgg tgatcactgc gttgggtgcc
    24181 gatcgccctg gaattgtgaa caccatcacc cgtcatgtca gtagttgcgg ctgtaatatt
    24241 gaagacagtc gcctggcgat gctgggagaa gagttcacgt ttatcatgct gctttccggt
    24301 tcatggaatg ccatcactct gattgaatca acgttaccgt tgaaaggtgc cgaactggat
    24361 cttttaatcg tgatgaagcg cacgacggcg cgtccgcgtc cgccaatgcc agcatctgtc
    24421 tgggttcagg tcgatgttgc agactccccg catttaattg aacgcttcac agcacttttc
    24481 gacgcgcatc atatgaacat tgcggagctg gtgtcgcgca cacaacctgc tgaaaatgaa
    24541 cgggctgcgc agttgcatat tcagataacc gcccacagcc ccgcatccgc ggacgcagca
    24601 aatattgagc aaacgttcaa agccctatgt acagaactca atgcacaagg cagtattaac
    24661 gtcgtcaatt attcccaaca tgatgaacag gatggagtta agtaatgaat ccactgaaag
    24721 ccggtgatat cgcaccgaaa tttagcttgc cggatcaaga cggagaacaa gttaatttga
    24781 ccgacttcca gggacagcgt gttctggttt atttctaccc gaaagccatg acccccggct
    24841 gtaccgtaca ggcctgcggc ttacgcgata acatggatga gttgaaaaaa gcgggcgttg
    24901 atgtgctggg tatcagcacc gataaacccg aaaaactctc ccgttttgcg gaaaaagagc
    24961 tgcttaactt tacgctcctg tctgatgagg accaccaggt gtgcgaacaa ttcggcgtct
    25021 ggggtgaaaa gtccttcatg ggcaaaacct acgatggtat tcatcgcatc agcttcctga
    25081 ttgacgctga tggcaaaatc gaacatgtct ttgacgattt caaaaccagc aatcaccacg
    25141 acgttgtgct gaactggctg aaagaacacg cctgattact ttgctccatt ccgtgctggc
    25201 tgcgcttgcg gccagcatac ctcacttctc gtgatcaaga tcacattctc gctttcccct
    25261 gcgacacggg tgtcgaatcc attttttgct gaacgttaat gaccatcatt tttgtaccgt
    25321 tcagaatcca gttaatacat aacttattga atagattgag ttaatcagaa tggcatcctt
    25381 tatacaatat gaaatgcaat gtttcatatc attttcaagg agccgacatg aaccgctttg
    25441 tggtggccga accactgtgg tgtacaggat gtaatacctg tctcgctgcc tgttcggacg
    25501 tgcataaaac gcaaggttta cagcaacacc cgcgcctggc cctggcgaag acgtcaacaa
    25561 tcactgcccc tgtcgtgtgt catcactgtg aggaagcccc ttgcctgcag gtctgcccgg
    25621 tcaatgccat ctctcagagg gatgatgcga tccaactcaa cgaaagcctc tgtattggct
    25681 gcaagctttg cgccgtggtc tgcccatttg gcgcaatcag cgcttcagga agccgtccgg
    25741 tgaatgccca tgcgcaatat gtttttcagg ctgaaggctc actcaaagac ggcgaagaaa
    25801 acgtgccgcc ccaacatgcg ttgctgcgct gggaaccggg tgtccagacc gtcgcggtga
    25861 aatgcgacct gtgtgatttc ttgccagaag gtccggcctg cgttcgcgct tgcccgaatc
    25921 aggcgttacg gctgatcacc gacgatagcc tgcaacgtca gatgaaagaa aaacagcgcc
    25981 ttgccgcaag ctggtttgcc aatggcgggg agtctcccct ttccctcact caggagcaac
    26041 actaatggat gccctgcaat tattaacctg gtcgctgatc ctctatctgt ttgccagtct
    26101 ggcttcgctg tttttactcg gtctggacag actggctatt aagctttccg gcatcacatc
    26161 gctggtgggc ggcgtgattg gcatcatcag cggaattacg caattacatg ctggtgtaac
    26221 tttagtcgcc cattttgcca ccccttttga ctttgccgat ttaaccctgc gaatggatag
    26281 cctctcggca tttatggtgc tagttatctc cttgctggtg gtggtttgtt cgctctattc
    26341 attgacttat atgcgcgaat acgagggcaa aggcgcggcg gcgatgggct tctttatgaa
    26401 tcttttcatc gcatcgatgg ttgccctgct ggtgatggac aacgcttttt ggttcatcgt
    26461 gctgtttgaa atgatgtcgc tgtcttcctg gtttctggtc attgccaggc aggataaaac
    26521 gtcgatcaac gctggcatgc tctacttttt tatcgcccac gccggatcgg tgctgattat
    26581 gatcgccttc ttgctgatgg ggcgcgaaag cggcagcctc gattttgcca gtttcgcacg
    26641 ctttcacttt ctccggggct ggcgtcggcg gtgtttctgc tggccttttt cggttttggc
    26701 gcgaaagccg ggatgatgcc gttgcacagc tggttgccgc gcgctcaccc tgccgcacca
    26761 tcgcacgctt cggcgttgat gtctggcgta atggtcaaaa tcggtatttt cggcatcctg
    26821 aaagtagcga tggatctgct ggcgcaaacg ggtttgcctc tgtggtgggg cattctggtg
    26881 atggcgatcg gcgcaatctc cgcgctcctg ggcgtgctat atgcgctggc ggaacaggat
    26941 atcaaacggc tgctggcctg gagtaccgtc gaaaacgtcg gcattatttt gctggcagtc
    27001 ggtgtggcga tggacggtct gtcactgcac gacccgctgc tcaccgtggt tggactgctc
    27061 ggcgcactgt ttcatctgct caaccatgcg ctgttcaaag ggctgctatt tctcggcgcg
    27121 gacgcgatta tttcgcgttt gcatacccac gacatggaaa aaatgggggc actggcgaaa
    27181 cggatgccgt ggacagccgc agcatgcctg attggttgcc tcgcgatatc agccattcct
    27241 ccgctgaatg gttttatcag cgaatggtac acctggcagt cgctgttctc actaagtcgt
    27301 gtggaagccg tagcgctaca acttgcgggt cctattgcta tggtaatgct ggcagtcact
    27361 ggtgggctgg cagtaatgtg cttcgtcaaa atgtacggta ttactttctg tggtgcgccg
    27421 cgcagtacac acgctgaaga ggcacaggaa gtgccaaata cgatgatcgt cgccatgcta
    27481 ctgctcgcgg cactctgcgt attaattgcg cttagtgcca gttggctggc accgaagata
    27541 atgcatattg cccatgcgtt taccaatacc cctcccgtca ctgtcgccag cggaatagca
    27601 cttgtacccg gcacgtttca tacacgggtc actccttcat tactgttgct gttactactg
    27661 gcgatgcctt tgctgccagg cctttactgg ctgtggtgtc gttcgcgccg cgcagcgttt
    27721 cgtcgcacag gagatgcctg ggcatgcggc tacggctggg aaaacgcgat ggctccgtca
    27781 ggcaatggcg tgatgcagcc gctgcgtgtg gtcttctctg cgttatttcg tctacgacaa
    27841 cagctcgacc ctacgctgag gctaaataaa ggtcttgcgc acgtcaccgc cagggctcag
    27901 agcacagaac ccttctggga tgagcgggtg atccgcccca tcgtgagcgc cacccaacgg
    27961 ctggtcaaag aaatacagca tctgcaaagc ggcgactttc gtctctattg cctgtatgtg
    28021 gtcgccgcac tggttgtgct gctaatcgct attgccgtct aaggaaatca ccatgagaca
    28081 aactctttgc gacggatatc tggtcatttt tgcgttagca caggccgtga ttctgctgat
    28141 gctaacccca ctttttacgg gtatttcccg gcagatacgc gcgcgtatgc actcccgccg
    28201 cgggccgggg atctggcagg attatcgcga tatccacaaa ctgtttaaac gccaggaagt
    28261 tgcgccgaca tcttcaggtc tgatgttccg cctgatgccg tgggtattaa tcagcagcat
    28321 gctggtgctg gcgatggcct taccactatt tattaccgtt tccccttttg cgggcggcgg
    28381 cgatctgatc acccttatct atcttcttgc cctgtttcgt tttttctttg ctctttccgg
    28441 gctggatacc ggaagtccgt ttgcgggagt cggtgccagt cgcgagttga cgctcggcat
    28501 tctggtcgaa ccaatgctta ttctctcact gctggtattg gcgctgatag caggttccac
    28561 gcatatcgag atgatcagca atacgctggc tatgggctgg aactcgccgc taaccaccgt
    28621 actggcgtta ctggcctgtg gttttgcctg cttcattgag atgggaaaaa ttccctttga
    28681 tgttgctgaa gcagaacagg aattacagga aggcccgctg accgaatatt ccggtgccgg
    28741 gctggcgcta gcgaaatggg ggctggggct gaaacaggtc gtgatggcag cactgtttgt
    28801 ggccctgttt ctgccctttg ggcgcgcgca agaactttct ctcgcctgcc tgctgacttc
    28861 acttgtcgtt acgctgctca aggttttgct gatttttgta ctggcctcaa tcgcagaaaa
    28921 cacgctggca cgcgggcgtt ttttactcat tcaccatgtg acctggcttg gcttcagcct
    28981 tgctgcgctt gcatgggtct tttggttaac cggtctgtaa ggagcactga cggaatatgg
    29041 aaaatcttgc tctgacgacg ttaatgctgc cttttatcgg cgcactggtc gtttcgtttt
    29101 cgccacaacg tcgggccgcc gaatgggggg ttttgttcgc cgcgctgacc acgctgtgca
    29161 tgttgtcgct gatctccgcg ttttatcagg ccgataaagt tgccgtcacg ttgacgttgg
    29221 tcaacgtggg ggatgtggca ttgtttggcc tggtcattga tcgcgtgagt acgctgattc
    29281 tgtttgtggt ggtgtttctc ggtttgctgg tcacgatcta ctccacgggt tatctgacgg
    29341 ataaaaatcg cgaacacccg cataacggca cgaatcgtta ttacgcattt ttgctggtgt
    29401 ttatcggcgc gatggcggga ctggtagtct cctcgacgct gctcggtcag ttgttgtttt
    29461 ttgaaattac gggcggctgc tcctgggcgt tgatcagtta ttaccagagc gataaagcgc
    29521 agcgttcagc actaaaagcg ttgcttatca cccatatcgg ttcgttgggg ttgtatcttg
    29581 ccgccgccac gctgtttttg cagaccggaa cgtttgtgct tagcgcgatg agcgagttac
    29641 atggcgacgc acgttatctg gtttatggcg gcatcctgtt tgccgcgtgg gggaaatcgg
    29701 cccagctacc gatgcaagcg tggctaccgg acgcaatgga agcgccaaca ccgatcagcg
    29761 cctatctcca cgccgcatcg atggtgaaag tgggcgttta catttttgcc cgcgccatta
    29821 tcgacggcgg caatatcccg catgtgattg gcggcgttgg catggtcatg acactggtca
    29881 ccattcttta tggctttctg atgtatttgc cacagcagga tatgaagcgg ttgctggcct
    29941 ggtcgaccat cactcaactt ggctggatgt tcttcggctt gtcgctctcc atcttcggct
    30001 cgcggctggc gctggagggt agcatcgcct acatcgtcaa ccacgcgttc gctaaaagcc
    30061 tgtttttcct tgtagcaggt gcgctgagtt acagctgcgg cacgcgcttg ttgccgcgtc
    30121 tgcgtggcgt attgcacacc ctgccgttgc caggcgtggg tttctgcgtg gcagcgctgg
    30181 caattaccgg tgtgccgccg ttcaacggct tcttcagtaa attcccgctg tttgccgccg
    30241 gttttgcgtt gtcagtggag tactggatcc tgctgcccgc catgattctg ctgatgattg
    30301 aatcggtcgc cagtttcgcc tggtttattc gctggtttgg tcgcgtcgtg ccgggcaaac
    30361 cgagcgaggc cgtcgccgat gccgcaccgc tgccagggtc aatgcgcctg gtgttgattg
    30421 tactgattgt gatgtcgctg atttccagcg taatcgccgc gacctggttg cagtaaggag
    30481 atgatgaatg accggttcta tgatcgtaaa taatctggcg ggactgatga tgctgacatc
    30541 gctgtttgtg attagcgtca aaagctatcg cctgtcatgc ggattttacg cctgccagtc
    30601 gctggtgctg gtgtctattt tcgccactct ctcgtgcctg ttcgccgcag agcaactgct
    30661 gatctggtcc gccagcgcct ttatcaccaa agtgctgctg gtaccgttaa tcatgactta
    30721 cgctgcacga aatattcccc agaacatccc ggaaaaagcg ttattcggtc cggcaatgat
    30781 ggcactgctc gcggcgttaa ttgtcctgct ttgcgcattt gtcgttcagc ccgtgaagct
    30841 accgatggct accgggctga aaccggcgct ggcggtagcg ttaggtcatt ttctgcttgg
    30901 cctgctgtgc attgtcagcc aacgcaatat cctgcggcaa atttttggtt actgcctgat
    30961 ggaaaacggc tcccatctgg tgctggcgct tcttgcctgg cgagcaccgg aactggtgga
    31021 aataggtatc gctaccgacg ccatcttcgc cgtcattgtg atggtgttac tggcaagaaa
    31081 aatatggcgt acccacggca cgctggacgt gaacaacttg accgcgctga agggataatg
    31141 agatgagtta ttctgtgatg ttcgctttac tcctgctcac gccgctgctt ttttcgctgc
    31201 tctgttttgc ctgccggaaa cgggggctct ctgcgacttg tacggtgact gtattacata
    31261 gcttagggat cacactgctg ctgattctgg cactctgggt ggtccaaact gccgctgatg
    31321 caggagaaat attcgctgcg ggactgtggc ttcatattga tggtctgggc ggtttgttcc
    31381 tcgccattct tggtgtgatt ggctttctca ccggtgttta ctcgattggc tacatgcgtc
    31441 atgaagtggc acacggcgag ctttcacccg ttacactgtg cgattactac ggtttcttcc
    31501 atctgttttt gttcaccatg ctgctggttg ttaccagcaa taacctgatt gtgatgtggg
    31561 cggcgatcga agccaccacc ttaagctcgg cgtttctggt aggcatttac ggtcagcgtt
    31621 catcgctgga atcagcatgg aagtacatca ttatttgtac tgttggtgtc gcttttggtc
    31681 tgttcggtac cgtgctggta tacgccaacg ccgccagcgt tatgccgcag gcagaaatgg
    31741 cgatattctg gagcgaggtt cttaagcaat cgtccttgct tgacccaaca ttaatgctgt
    31801 tggcctttgt gtttgtgcta attggctttg gtaccaaaac cgggctattt cccatgcacg
    31861 cctggctgcc ggatgctcac agtgaagcgc cgagtccggt cagcgccctg ctctccgccg
    31921 tattgctgaa ctgcgcgctg ttggtgctga ttcgctatta catcattatt tgccaggcca
    31981 tcggcagcga tttccccaac cggttgttgc tcatcttcgg catgttgtcg gttgccgtgg
    32041 cggcattttt cattctggta cagcgggaca ttaagcgtct gctggcgtac tccagcgtgg
    32101 agaacatggg gctggtcgcg gtggcgctag gcattggcgg gccgctggga atttttgccg
    32161 cgctgctgca caccttaaac cacagtctgg caaaaacgct gctgttctgc ggttccggca
    32221 atgtactgct caagtacggc acgcgcgatc tcaacgtcgt ctgtgggatg ctcaaaatca
    32281 tgccatttac cgccgtgctg tttggcggcg gtgcgctggc gctggcgctg gcagggatgc
    32341 cgcccttcaa catttttctt agcgaattta tgaccgttac cgcaggactg gcacgtaatc
    32401 acctgctgat tatcgtcctg ctgttattgc tgttaacgct ggtgctggcg ggcctggtac
    32461 ggatggctgc gcgggtgtta atggcgaaac cgccgcaggc cgttaaccgg ggtgatctcg
    32521 gctggttgac cacctcgcca atggtgattc tgctggtcat gatgctggcg atgggaacgc
    32581 atattccaca acctgtcatc aggatcctgg cgggcgcttc cactatagtc ctctcaggga
    32641 cgcacgacct gcctgcacaa cgtagcacct ggcatgattt tttgccttca ggcaccgcat
    32701 ctgtttcgga gaaacacagt gaacgttaat tcatcgtcaa atcgtggcga agcgattctc
    32761 gccgccctga aaacgcagtt ccccggcgcg gtgctggatg aagagcgaca aacgcctgaa
    32821 caggtcacca ttacggtgaa aatcaatctg ctgcctgacg ttgtacatta tctttattat
    32881 caacatgatg gctggcttcc agtcctgttt ggcaacgacg agcggacact taacggtcat
    32941 tacgcggttt attatgccct ttcaatggaa ggggccgaaa aatgctggat cgtggtgaag
    33001 gcactggtcg atgccgacag tcgggagttt ccgtcagtca caccgcgcgt ccctgccgcg
    33061 gtctggggcg agcgagaaat tcgcgatatg tacgggctga ttccggttgg cctgccggat
    33121 cagcgtcgcc tggtgttgcc cgatgactgg ccggaagata tgcatccgct gcgcaaagat
    33181 gcgatggatt atcgactgcg ccctgaaccg acgacagatt ccgaaacgta tgcgtttatc
    33241 aatgagggca acagcgatgc gcgggtgatc cctgtcggcc cgctgcatat cacctcctat
    33301 gaaccgggtc acttccgctt gtttgtggat ggcgagcaaa ttgtcgatgc tgattaccgc
    33361 ctgttttatg tccatcgcgg catggagaaa ctggcagaaa cgcggatggg ctacaacgaa
    33421 gtgaccttct tatcggaccg cgtgtgtggg atttgcggtt ttgcccacag tgtggcctat
    33481 accaactcgg ttaaaaatgc actggggatt gaggtgccgc aacgagcaca tactattcgc
    33541 tcgattctgc tggaagtcga gcggctacac agtcatttgc tcaaccttgg cctctcctgc
    33601 cattttgttg gttttgatac cggctttatg caatttttcc gcgtgcggga aaagtcgatg
    33661 acgatggcgg aattgctgac cgggtcgcgt aaaacctacg gtctgaatct gattggtggt
    33721 gttcgccgcg atattctcaa agagcaacgt ctgcaaacgc tgaaactggt gcgcgagatg
    33781 cgcgccgacg tgtcggagct ggtagagatg ctgcttgcta cgccgaatat ggaacaacgc
    33841 actcagggca ttggcattct cgaccgacaa atcgcccgtg attatagccc tgtagggccg
    33901 ctgatccgcg cagtggtttt gcccgtgatt tgcgctttga tcacccctac gccgactacg
    33961 gcaatattcc aaaaacactg tttaccttta ccggcggcga tgttttctcc cgcgtgatgg
    34021 tccgtgtcaa agagacgttt gattcgctgg caatgctgga atttgccctc gacaacatgc
    34081 cggatacccc actgctgacc gaaggcttta gctataaacc tcacgcattc gcgctgggct
    34141 ttgttgaagc gccacgcggt gaagacgtgc actggagcat gctcggtgat aaccaaaaat
    34201 tgttccgctg gcgctgtcgt gccgccacct acgccaactg gccggtgttg cgttacatgc
    34261 tgcgcggcaa taccgtttct gacgcaccgc tgattatcgg tagccttgat ccctgctact
    34321 cctgtaccga ccgtgtgacg ctggtagatg tgcgcaagcg ccagtcaaaa accgtgctgt
    34381 ataaagagat cgaacgctac ggcattgatc gtaaccgttc gccgctgaag taaggacaga
    34441 agatgctgaa gttactgaaa actattatgc gcgccggaac cgcgacggtg aaatatccct
    34501 tcgcgccact ggaggtcagc ccaggctttc gcgggaaacc ggacctgatg cccagccaat
    34561 gtattgcctg cggtgcctgc gcctgtgctt gtccggcaaa tgcgctgact atccagaccg
    34621 acgaccagca aaattcgcgc acctggcagc tctatctggg gcgttgtatt tactgcggac
    34681 gttgtgaaga agtgtgcccg accagagcca tccagcttac caataacttt gaactgaccg
    34741 tcacccataa agccgatctc tatacccacg cgacgttcca tctacaacgt tgcagccgtt
    34801 gcgaacgccc gtttgccccg caaaaaaccg tcgcactggc tgctgaattg ttggcacagc
    34861 aacaaaatgc tccacaaaac cgcgaaatgt tatgggcgca agcgagcgtc tgcccggagt
    34921 gcaaacaacg cgcgacgctg atcaacgacg atacagatgt accgctggtg gctaaggagc
    34981 agctatgagt ccagtgctta cgcaatatgt cagccagccc atcacgctgg acgagcaaac
    35041 gcaaaagatg aagcggcatt tgctacagga tatccgtcgc tcggcttacg tttatcgcat
    35101 cgattgcggc ggctgcaacg cctgtgaaat cgaaattttt gctgccatca cccccgtatt
    35161 cgacgcagaa cgttttggca ttaaggttgt ttcatcaccg cgtcacgccg atattttgtt
    35221 atttactggc acagtcaccc gggcgatgcg tatgcctgca cttcgggcgt atgagtctgc
    35281 ccccgatcat aaaatctgtg tttcctacgg cgcgtgcggt gtcggcggcg gtattttcca
    35341 cgatctctac agcgtctggg gcggcagcga caccattgtc cccattgatg tttggatccc
    35401 cggctgcccg ccaacaccgg ccgccaccat tcacggtttc gccgtggcgc tcggtttgct
    35461 gcaacagaag attcacgcgg tggattatcg cgatcccacc ggggtgacta tgcaaccgtt
    35521 gtggccgcag atcccgccat cacagcgtat cgccattgag cgagaagcgc ggcggctggc
    35581 gggctatcgt caggggcgag aaatttgcga tcggctcctg cgccatttaa gcgacgatcc
    35641 tacaggaaat cgggttaaca cctggttgcg cgatgccgac gatccacgtc tcaatagtat
    35701 cgttcagcaa ctctttcgcg tactccgggg gttacatgac tgaagagtgc ggggaaattg
    35761 ttttctggac gctgcgaaaa aagtttgtcg ccagtagcga cgagatgccg gaacacagct
    35821 ctcaggtaat gtattactcg ctggctatcg gtcatcacgt tggcgtgatt gattgtctga
    35881 atgtcgcctt ccgctgccca ctgacggaat acgaagattg gcttgcactg gtcgaagagg
    35941 agcaagcccg acgtaagatg ctgggggtga tgacttttgg tgagattgtt attgacgcca
    36001 gccacaccgc cctgttgacc cgggcattcg cgccactggc agatgacgcg acgtctgtgt
    36061 ggcaagcgcg tagcattcaa ttcattcatc tgttggatga aattgtgctg gaaccggcta
    36121 tctatctgat ggccagaaaa attgcgtgag aaggatttct cattaataag gactgttgat
    36181 ggctatgtca gacgaagcga tgtttgcccc gccgcaagga ataacaattg aagcggtaaa
    36241 cggaatgctc gcggagcggt tagcgcagaa acacggcaag gcgtctttat tacgcgcctt
    36301 catcccgctg ccgccgccgt tcagcccggt acaacttatt gaactgcatg ttctcaaaag
    36361 caacttctat taccgctacc atgatgatgg cagcgatgtg acggcaacaa cagagtatca
    36421 gggcgagatg gtcgattatt cgcgttacgc cgtccttctc ggcagtagtg gaatggcgga
    36481 gctacgcttt attcgtaccc acggcagccg ttttactccc caggattgca cactgtttaa
    36541 ctggcaggca cgcattatca ccccggttct gcaatcatgg ctcaatgatg aagcacagca
    36601 ggtggcgctg cgtttgctgg agaaagatcg cgatcatcat cgggtactgg tggatatcac
    36661 taatgcagtg ctgtcacatc ttgatctcga cgatctgatc gctgacgtcg ctcgtgagat
    36721 ccatcatttt ttcggtctgt cttcagtcag tatggtactg ggcgatcatc gaaagaacga
    36781 gaagttcagc ctgtggtgca gcgatctttc tgcctcacat tgtgcgtgtc tgccacacaa
    36841 tatgcctggc gacagtgtat tgctgacaca aacgctacaa acccgacaac cgaccttgac
    36901 gcaccgtgca gatgatctgt ttctctggca acgcgacccg ttattactct tacttgcatc
    36961 taacggctgc gaatctgcgc tccttatacc gcttaccttt ggcaaccata caccgggtgc
    37021 attgttgctg gcgcatacct cttccactct ctttagtgag gaaaactgcc agctactaca
    37081 acacatagcc gatcgcatcg ctattgccgt tggcaatgcc gatgcctgcc gtagaatgac
    37141 cgatttgcag gaaagtttgc agcaagaaaa ccaccagctt agcgagcagc tcctttcgaa
    37201 tctgggcgtc ggtgacatta tctatcaaag ccaggcaatg gaagacctgc tccagcaggt
    37261 agatattgtg gcgaagagcg acagtacggt gttgatttgc ggtgaaaccg gaaccggcaa
    37321 agaggtgatc gccagagcga tccatcaact tagcccgcga cacgacaagc cgctggtcaa
    37381 aatcaactgc gctgccatcc ccgccagtct tctggaaagt gagttattcg gtcatgacaa
    37441 aggggcgttt actggtgcga ttaataccca tcgtggtcgt tttgaaattg ccgatggcgg
    37501 cacgttgttt ctcgatgaaa ttggcgatct gccgttagaa cttcagccta aactgctgcg
    37561 cgtattgcag gaacgggaga ttgagcgtct cggcgggagt agaacgatcc cggtaaatgt
    37621 cagagtcatt gccgccacca accgtgattt gtggcaaatg gttgaagatc gccagtttcg
    37681 cagcgatctc ttttatcgcc tgaatgtctt cccactggaa ttgcctccgc tgcgcgaccg
    37741 tccggaagat atccctcttt tagcaaagca tttcacgcaa aaaatggcgc gccatatgaa
    37801 tcgttcaatt gacgccatcc cgaccgaggc gctacgccag ttgatgtcgt gggactggcc
    37861 gggcaacgtg cgcgagctgg aaaacgtgat tgagcgagcg gtactgctga ctcgcggtaa
    37921 cagtctgaat ttacatctta atgtccgaca aagccgttta ctgccgacgc taaatgaaga
    37981 ttcagcgctt cgcagttcaa tggcgcagtt gctgcacccg acgacgccag agaatgacga
    38041 agaagaacgt cagcgcattg ttcaggtatt gcgagaaacc aatggcattg ttgccgggcc
    38101 ccgtggcgcg gcgacacgat tagggatgaa gcgcaccacg ctgctgtcac gaatgcagcg
    38161 tctgggaatc tcggttcgcg aggtgttgta atctgctttt gcaggagtat gcatgagaaa
    38221 caaactctct ttcgacttgc agttgagcgc cagaaaagcg gcaatcgctg aacggattgc
    38281 cgcccataaa attgcccgca gtaaagtgtc ggtcttttta atggcgatgt ccgctggcgt
    38341 gtttatggcg atcggattta ctttttacct ttccgttatc gccgatgccc cgtcttcata
    38401 ggcattaacc catctggtgg gcggcctttg ctttacactt ggctttattt tgctggcggt
    38461 ttgcggcacc agcctgttca cctcgtcggt aatgacggta atggcaaaaa gtcggggcgt
    38521 tattagttgg cgaacttggc tgattaacgc acttctggtg gcctgcggta atctggcagg
    38581 tattgcctgt ttcagtttgt taatctggtt ttccgggctg gtgatgagtg aaaacgcgat
    38641 gtggggagtc gcgattttac actgcgccga gggcaaaatg catcatacat ttactgaatc
    38701 tgtcagcctc ggcattatgt gcaatctgat ggtttgcctg gcgctgtgga tgagttattg
    38761 cggacgttcg ttatgcgaca aaatcgtcgc catgattttg tccatcaccc tgtttgtcgc
    38821 cagtggcttt gagcactgta tcgccaattt gtttgtgatt ccgttcgcca ttgccattcg
    38881 ccatttcgcc cctacctcct tctggcaact ggcgcacagt agcgcagaca attttccggc
    38941 actgacggtc agccatttta ttaccgccaa tctgctcccg gtgatgctgg gtaatattat
    39001 cggcggtgca gtgctggtga gtatttgtta tcgggctatt tatttacgtc aggaatcctg
    39061 aaaaatcagc ccggcgaaac agttcgtcgg gctgaagatt attcttgcgc gatttgccca
    39121 tcgggccagg cgtggatcac ggctttgatc agcgtcgcca atggaatggc gaagaatacg
    39181 ccccagaatc cccacaaacc accgaagatc accaccgata aaataatcac cagcggatgc
    39241 aggttaaccg cttcggagaa caacaccggt actagcaggt tgccgtccag cgcctgaata
    39301 atcagataca ccgcgaagca gctccagaat tcagtacctg cgccaaactg gaatagcgcc
    39361 acgccaacca ccggaatggt caccacaaat gcgccaatgt acggaataag aaccgagaag
    39421 ccgaccagca ctgccagcag cagcgaatag ttcagcccga agagcaagaa ccccagccag
    39481 gtggcgatcc ccaccacgat catctccagc actttgccgc ggatatagtt ggtgatttgt
    39541 tgattcatct ccttccacac ctgtcctgcc agcccacggt tacgcggcag cacccggcga
    39601 acggcgttca gcatctgctc tttgtctttc agcaggaaga agaccatcaa tggcaccagc
    39661 accagataga cggctatggt cagcaaaccg accagcgagg cgagggaaat tttcaccacc
    39721 gaatcgccca tggtcagcat ccgactgcgc atattttcgg ccattgcatc aataatgccc
    39781 gcgtccatta acaccggata gcggcgcggc aacgtggcgg caaagtcaga aagcttattg
    39841 agcatccccg gcatatcgcg gattaagtag atgccctgtt gccaggcgat aggcagtacc
    39901 acgaacgcca tcagtagcaa tataccgaca aaaaccacca atacaatcga cgtcgcccag
    39961 cggcgggagc agccaataga ttgcaggcgc acggttggcc attccagcaa ataagccagc
    40021 acaatagcca ccagcaacgg agcaagcagg ccactaaaga aaaagataat gccaaatccg
    40081 gcaactaaaa taaccagcaa ggcaatcgct tccgggtcgc taaaacggcg gcgataccat
    40141 tgcatcaaca tttcgagcat aaagaccttc cctgaacctc aagagcggga ttgcgatccg
    40201 caattgtatc gaaatgtcac aaaaaagact tcgcttttta tgacggattc aggaaactaa
    40261 aaagtcattt gagtgggcta atcttcgccg ttacactcaa aggcggcgag gtgggaacga
    40321 tatttcacag tatcggtcaa atgactatct tcagaaatac aggatagagg ttatgttcag
    40381 gcagttgaaa aaaaacctgg ttgcaaccct cattgctgct atgaccattg gtcaggtagc
    40441 cccggcgttt gccgacagcg cagacacctt gccggatatg ggaacctccg caggaagcac
    40501 gctttccatt ggtcaggaaa tgcagatggg cgactattat gtccgccagc tacgcggcag
    40561 cgcgccgtta attaatgacc cgctgttaac gcaatatatt aattcgctgg ggatgcgtct
    40621 ggtttcgcat gccaattcgg ttaagacacc gtttcatttt tttctgatca acaacgacga
    40681 aattaacgcc tttgctttct ttggcggcaa cgtggtgctg cactctgccc tgttccgtta
    40741 ttccgataac gaaagtcaac tggcttcagt tatggcgcac gaaatctccc acgtcaccca
    40801 acgtcacctg gcgcgagcga tggaagatca acaacgcagc gcgccgctga cctgggtcgg
    40861 cgcgttaggt tctattttac tggcgatggc cagtccgcag gcggggatgg cggcgctgac
    40921 cggtacactg gcgggaacgc gccaggggat gatcagtttc acccagcaaa atgaacagga
    40981 agcggaccgc attggtattc aggtgctgca acgctcggga ttcgatccac aggcgatgcc
    41041 gaccttcctg gaaaaattgc tcgatcaggc gcgttactcc tcgcgcccgc cggaaatttt
    41101 attgactcac ccgttgccgg aaagtcgtct ggcagatgcc cgcaaccgtg ctaatcagat
    41161 gcgcccgatg gtggtgcagt cgtcggaaga tttctatctg gcgaaagcgc gcacactggg
    41221 gatgtataat tccggacgta accagctcac cagtgatttg ctggatgaat gggcgaaagg
    41281 aaacgttcgt cagcaacgag cggcgcaata tggtcgtgct ttacaggcga tggaagccaa
    41341 taaatacgac gaggcgcgta aaacgctgca accgttactg gcggcagaac ctggcaacgc
    41401 atggtatctc gatctggcta ccgatatcga tcttgggcaa aacaaagcca atgaggcaat
    41461 caatcgcctg aaaaatgccc gcgatttacg caccaatccg gtgttgcagc tcaacctggc
    41521 gaacgcttat ctgcaaggcg gtcaaccaca agaagcggcc aatattctga atcgctacac
    41581 ctttaataat aaagatgaca gcaacggctg ggatttgctg gcacaggcgg aagccgcgct
    41641 aaataaccgc gatcaggagc tggctgcgcg agcagaaggt tatgcgctcg ccggacgact
    41701 cgatcaggcc atttcgctgt tgagtagcgc cagttcgcag gtaaaattag gcagcctgca
    41761 acaagcgcgt tacgatgcgc gcatcgacca gttgcgccag ctgcaggaac gctttaagcc
    41821 ttataccaag atgtaattcg cattaaagga agaacaatga ccaaacaggt aaaaatctac
    41881 cataacccac gctgttcaaa gagccgggaa acgctgaacc tgctgaaaga aaacggcgtg
    41941 gagccagaag tggtgctcta ccttgagaca cccgccgatg cggcaacgct gcgcgatttg
    42001 ctgaaaatgc tggggatgaa tagcgcccgt gaattgatgc gccagaaaga ggatctttat
    42061 aaagaactaa acctggctga cagttcgctt agcgaagagg cgctgattca ggcaatggtg
    42121 gataatccaa agctgatgga gcgcccgatt atggtcgcga atggcaaagc gcggattggt
    42181 cgtccaccgg agcaggtact ggaaatcgtg ggttaagatg tgctccatcg ctagttgaag
    42241 cacattgccg gatgcgacgc ttgccacgtc ttatccggcc tacgaatact atggattaac
    42301 ttcgcgtagg tcaggcgttc gcgccgcatc cgacaataaa caccttatct acaacttcag
    42361 aatttctttc acaaacggaa tggtcagctt acgttgcgcg gtaatcgacg cacgatccaa
    42421 ctgatccaac gtcataaata gcgtgcgcat ttctctgtcg agccgcttca gcaagaaacg
    42481 ccccacatct tccggcagtt caaaaccacg caaacgcgcg cgtaactgta gcgcctgcaa
    42541 cttatcttca tcagaaagtg gctgcaattt gtagatctgc ccccagtcga gacgcgacgc
    42601 gagatccggt aatcccagat tcaactgccg cggtggacga tcgccggtga tcaacaaccg
    42661 tgttttgccc gattccagaa ttcgattgta gagatcgaaa atcgccatct cccacaactc
    42721 atcgcctgca atacactcaa tgttgtcgat acagaccagc gacaaatgct ccataccgtc
    42781 gagcacttcc ggaacaaacc aggtgcgttt atccagcggg acatagccca ccgcatcgcc
    42841 acgctgcgac aattccgcgc aagccgcgtg cagcagatgg ctgcgccccg cgccttcgcg
    42901 tgcccagaga tagatgtaac cgctatgttc ctgacgcagc acgttttgca gcgcggccag
    42961 taaagaggag ttatcccccg gccagaaact tgcaaaggtt tcgtcgtcag gaagataaag
    43021 tggcaaagag agctgtgccg gtgtgttcag agatacctca accaggattt cacaaaatcg
    43081 cgagaagttt accacagaat cccatgatgt ttgaaccggg cagcaacact gcccggtcgg
    43141 tacattattt gtctgttata tccgcgtctt ctgcgtccag caccacttct tcagggcgca
    43201 gcacactgat cagtttgaaa atcaaactta agccgatacc gacgatggtc gccagcgcca
    43261 taccttttaa ctccgccgcg ccgatgttta ccttcgcgcc actgacgccg atgatcaaaa
    43321 tcacggaagt caggatcagg ttctgtgctt tgttgtaatc cactttcgat tcgatcaaaa
    43381 cacgaatacc ggaagcacca atgacaccat aaagcagcag cgaaacgccg cccatcaccg
    43441 gcaatgggat catctggata gcggcagcca gtttaccgac gcaggaaagc aggatagcga
    43501 aaatcgccgc cccgccgata acccaggtac tgtaaacacg ggtgatcgcc atcacgccaa
    43561 tgttttctcc gtaagtggta tttggcgtag agccaaagaa gccggagatc acggtcgaca
    43621 ggccgttagc aaacatcgaa cggtgcagac ctggatcgcg cagcagatct tttttgacga
    43681 tattagccgt tactaccagg tgccctacgt gttcggcaat aaccactaac gccgctggca
    43741 gaatagtcag aatggcaaac cactcgaagc gcggcgtata gagggttggc agtgcaaacc
    43801 agtgagcatt aataatcggc gtggtatcga caattcccat tgcgaaagag agcgcgtacc
    43861 ccaccagcac gccaattaaa atcgggataa ttgccaggaa accacgaaac agcacggaac
    43921 ctaaaaccgt gaccgccagg gtggtaatag agatgatgat ggttttggag tttggcgttt
    43981 gcccttcagc cgggagtaaa cccgccatac cggcagctac gcccgccagc tccagaccga
    44041 tgacggcaac gattgcgccc attgccgcag gtggaaacag cacgtccaac cagccggtcc
    44101 ccgctttctt cacgataaaa gaaaccaggc agaacagcac gccgcacata ataaagccgc
    44161 ccagcgcgac ttcataccct aacggcaaca gtaacaatac cggtgaaata aaggcaaagc
    44221 tggaaccaag ataagccgga attttccctt tacagatgaa gagatacagc agcgttccaa
    44281 taccgttaaa taacagtaca gtcgccgggt taatatgaaa taagacgggc accaggacgg
    44341 ttgcaccaaa catggcgaac aaatgttgca aactaagcgg gattgtctgt aaaagtggcg
    44401 gtctttcact caccccgata gcacggcgcg tcatagtatt atcctctgta ttatgtgtta
    44461 taggcgcttt actcaaaaaa aagccgactc ttaaagtcgg ctttaattat ttttattctt
    44521 tatttcgtac caaagatttt gtcaccggca tcgccgaggc ccggaataat gtatccgtgc
    44581 tcgttcagtc cctggtcaat cgatgcagta tacagttcga cgtccgggtg cgctttttcc
    44641 agcgcagcga taccttctgg cgcagcgacc agcaccagaa ctttgatgct gctgcagccc
    44701 gcttttttca gcaggtcgat ggtcgcgata acggaaccac cggttgccag cattgggtca
    44761 acgatcagcg ccatacgctc atcgatgtta gaaaccagtt tctggaagta cggtaccggc
    44821 tccagcgttt cttcattacg gtacataccg acaacgctga tgcgcgcgct cggaacgttt
    44881 tccagcacac cgtccatcat accaagaccc gcacgcagaa ttggcacaac ggtaattttc
    44941 ttacctttga tctggtcgat ttctaccggg ccgttccagc cttcgatagt tactttttcc
    45001 gtttcgaggt cggcggtcgc ttcgtaagtc agcaggctac ccacttcgga agcgagttcg
    45061 cgaaagcgct tggtgctgat atcttgctca cgcatcagtc ccagcttgtg tttgacgagt
    45121 gggtgtttga cttccacgat cttcatactc tttctccttt gaggggcagc cacaaaaaaa
    45181 atcgacggat tatacctcct ttcttcaagg cggcaatatt attttcgttg actttagtca
    45241 aaatgataac agtttgagat aaagttattt tatattcaaa tggttatgaa agaagattat
    45301 tccatccgaa aactaacctt tgccctggca caagtcttct ttcgccgcgc gcccggggaa
    45361 aagacgtgca aaaaggttgt gtaaaacagt ctcgcaaacg tttgctttcc ctgttagaat
    45421 tgcgccgaat tttatttttc taccgcaagt aacgcgtggg gacccaagca gtgaccgata
    45481 aaacctctct tagctacaaa gatgccggtg ttgatattga cgcgggtaat gctctggttg
    45541 gaagaatcaa aggcgtagtg aagaaaacgc gtcgtccgga agtgatgggc ggtctgggcg
    45601 gcttcggtgc gctgtgtgca ttgccgcaaa aatatcgtga acccgtgctg gtttccggca
    45661 ctgacggcgt aggtaccaag ctgcgtctgg caatggactt aaaacgtcac gacaccattg
    45721 gtattgatct ggtcgccatg tgcgttaatg acctggtggt gcaaggtgcg gaaccgctgt
    45781 ttttcctcga ctattacgca accggaaaac tggatgttga taccgcttca gcggtgatca
    45841 gcggcatcgc ggaaggttgt ctgcaatcgg gctgttctct ggtgggtggc gaaacggcag
    45901 aaatgccggg gatgtatcac ggtgaagatt acgatgtcgc gggtttctgc gtgggcgtgg
    45961 tagaaaaatc agaaatcatc gacggctcta aagtcagcga cggcgatgtg ctgattgcac
    46021 tcggttccag cggtccgcac tcgaacggct attcgctggt gcgcaaaatt cttgaagtca
    46081 gcggttgtga tccgcaaacc accgaacttg atggtaagcc attagccgat catctgctgg
    46141 caccgacccg catttacgtg aagtcagtgc tggagttgat tgaaaaggtc gatgtgcatg
    46201 ccattgcgca cctgaccggc ggcggcttct gggaaaacat tccgcgcgta ttgccagata
    46261 atacccaggc agtgattgat gaatcttcct ggcagtggcc ggaagtgttc aactggctgc
    46321 aaacggcagg taacgttgag cgccatgaaa tgtatcgcac cttcaactgc ggcgtcggga
    46381 tgattatcgc cctgcctgct ccggaagtgg acaaagccct cgccctgcta aatgccaacg
    46441 gtgaaaacgc gtggaaaatc ggtatcatca aagcctctga ttccgaacaa cgcgtggtta
    46501 tcgaataatg aatattgtgg tgcttatttc cggcaacgga agtaatttac aggcaattat
    46561 tgacgcctgt aaaaccaaca aaattaaagg caccgtacgg gcagttttca gcaataaggc
    46621 cgacgcgttc ggccttgaac gcgcccgcca ggcgggtatt gcaacgcata cgctcatcgc
    46681 cagcgcgttt gacagtcgtg aagcctatga ccgggagttg attcatgaaa tcgacatgta
    46741 cgcacccgat gtggtcgtgc tggctggttt tatgcgcatt ctcagcccgg cgtttgtctc
    46801 ccactatgcc gggcgtttgc tgaacattca cccttctctg ctgccgaaat atcccggatt
    46861 acacacccat cgtcaggcgc tggaaaatgg cgatgaagag cacggtacat cggtgcattt
    46921 cgtcaccgat gaactggacg gtggcccggt tattttacag gcgaaagtcc cggtatttgc
    46981 tggtgatacg gaagatgaca tcaccgcccg cgtgcaaacc caggaacacg ctatttatcc
    47041 actggtgatt agctggtttg ccgatggtcg tctgaaaatg cacgaaaacg ccgcgtggct
    47101 ggatggtcaa cgtctgccgc cgcagggcta cgctgccgac gagtaatgcc cccgtagtta
    47161 aagcgccagc tctgccgctg gcgtttttca attcacctgt aaatcgcaag ctccagcagt
    47221 ttttttcccc cttttctggc atagttggac atctgccaat attgctcgcc ataatatcca
    47281 ggcagtgtcc cgtgaataaa acggagtaaa agtggtaatg ggtcaggaaa agctatacat
    47341 cgaaaaagag ctcagttggt tatcgttcaa tgaacgcgtg cttcaggaag cggcggacaa
    47401 atctaacccg ctgattgaaa ggatgcgttt cctggggatc tattccaata accttgatga
    47461 gttctataaa gtccgcttcg ctgaactgaa gcgacgcatc attattagcg aagaacaagg
    47521 ctccaacgct cattcccgcc atttactggg caaaattcag tcccgggtgc tgaaagccga
    47581 tcaggaattc gacggcctct acaacgagct attgctggag atggcgcgca accagatctt
    47641 cctgattaat gaacgccagc tctccgtcaa tcaacaaaac tggctgcgtc attattttaa
    47701 gcagtatctg cgtcagcaca ttacgccgat tttaatcaat cctgacactg acttagtgca
    47761 gttcctgaaa gatgattaca cctatctggc tgtggaaatt atccgtagcg atagcatccg
    47821 ttacgcgctg ctggagatcc catcagataa agtgccgcgc tttgtgaatt taccgccaga
    47881 agcgccgcgt cgacgcaagc cgatgattct tctggataac attctgcgtt actgccttga
    47941 tgatattttc aaaggcttct ttgattatga cgcgctgaat gcctattcaa tgaagatgac
    48001 ccgcgatgcc gaatacgatt tagtgcatga gatggaagcc agcctgatgg agttgatgtc
    48061 ttccagtctc aagcagcgtt taactgctga gccggtgcgt tttgtttatc agcgcgatat
    48121 gcccaatgcg ctggttgaag tgttacgcga aaaactgact atttcccgct acgactccat
    48181 cgtccccggc ggtcgttatc ataattttaa agactttatt aatttcccca atgtcggcaa
    48241 agccaatctg gtgaacaaac cactgccgcg tttacgccat atttggtttg ataaagccca
    48301 gttccgcaat ggttttgatg ccattcgcga acgcgatgtg ttgctctatt atccttatca
    48361 cacctttgag catgtgctgg aactgctgcg tcaggcttcg ttcgacccga gcgtactggc
    48421 gattaaaatt aacatttacc gcgtggcgaa agattcacgc atcatcgact cgatgatcca
    48481 cgccgcacat aacggtaaga aagtcactgt ggtggttgag ttacaggcgc gtttcgacga
    48541 agaagccaac attcactggg cgaagcgcct gaccgaagca ggcgtgcacg ttatcttctc
    48601 tgcgccgggg ctgaaaattc acgccaaact gttcctgatt tcacgtaaag aaaacggtga
    48661 agtggtccgt tacgcacaca tcgggaccgg gaactttaac gaaaaaaccg cgcgtcttta
    48721 tactgactat tcgttgctga ccgcagatgc gcgtatcacc aacgaagtac ggcgggtatt
    48781 taactttatt gaaaacccat accgcccggt gacatttgat tatttaatgg tgtcaccgca
    48841 aaactctcgc cgtctgttat atgaaatggt agaccgcgaa atcgccaacg cgcagcaagg
    48901 gctgcccagt ggtatcaccc tgaagctaaa taaccttgtc gataaaggcc tggttgatcg
    48961 tctgtatgcg gcctccagct ccggcgtacc ggttaatctg ctggttcgcg gaatgtgttc
    49021 gctgatcccc aatctggaag gcattagcga caacattcgt gccatcagta ttgttgaccg
    49081 ttaccttgaa catgaccggg tttatatttt tgaaaatggc ggcgataaaa aggtctacct
    49141 ttcttccgcc gactggatga cgcgcaatat tgattatcgt attgaagtgg cgacaccgct
    49201 gctcgatccg cgcctgaagc agcgggtgct ggacatcatc gacatattgt tcagcgatac
    49261 ggtcaaagca cgttatatcg ataaagaact cagtaatcgc tacgttcccc gcggcaatcg
    49321 ccgcaaagta cgggcgcagt tggcgattta cgactacatc aaatcactcg aacaacctga
    49381 ataaccctat gccaatacac gataaatccc ctcgtccgca ggagtttgct gcggtcgatc
    49441 ttggttcaaa cagttttcac atggtcatag cccgtgtggt agatggtgcc atgcagatta
    49501 ttggccgcct gaaacagcgg gtgcatctgg cggacggcct ggggccagat aatatgttga
    49561 gtgaagaggc aatgacgcgc ggtttgaact gtctgtcgct gtttgccgaa cggctacaag
    49621 ggttttctcc tgccagcgtc tgtatagtcg gcacccatac gctgcgtcag gcgctgaacg
    49681 ccactgactt tctgaaacgc gcggaaaagg tcattcccta cccgattgaa attatttccg
    49741 gtaatgaaga agcccgtctg atttttatgg gcgtggaaca tacccaaccg gaaaaaggtc
    49801 gcaaactggt tattgatatt ggcggcggat ctacggaact ggtgattggt gaaaatttcg
    49861 aacctattct cgttgaaagc cgccggatgg gttgtgtcag ctttgcccag ctttattttc
    49921 ctggcggggt catcaataaa gagaattttc agcgcgctcg catggcggca gcacaaaaac
    49981 tggaaacttt aacctggcaa ttccgtattc agggctggaa cgttgcaatg ggcgcttccg
    50041 gtaccataaa agccgcccat gaagtgttaa tggaaatggg cgagaaagac gggataatta
    50101 ccccggaacg tctggaaaaa ctggtaaaag aaattttacg tcaccgtaat ttcgcatcgc
    50161 tgagtttacc gggtctttcc gaagagcgga aaacagtctt cgttcctgga ctggcgattt
    50221 tatgtggtgt gtttgatgct ttagccatcc gtgaactgcg cctttctgac ggggcgttac
    50281 gcgaaggcgt actgtatgaa atggaaggac gtttccgtca tcaggatgtg cgtagtcgca
    50341 ccgccagcag cctcgccaac cagtatcaca tagacagcga acaggcccga cgagtgctgg
    50401 ataccactat gcaaatgtac gaacagtggc gggaacagca accgaagctg gcgcatccgc
    50461 aactggaggc gctactgcga tgggccgcca tgctgcatga ggtcgggttg aatatcaacc
    50521 acagcggttt gcatcgccac tccgcttata ttctgcaaaa cagtgacttg ccgggtttta
    50581 atcaggaaca gcagctgatg atggcgacac tggtgcgcta tcaccgtaaa gcgattaagc
    50641 tcgacgatct gccgcgcttt accttgttta agaagaaaca gttcctgcca ctgatacagc
    50701 tattgcgcct tggcgtatta ctaaacaatc aacgtcaggc aaccaccaca ccgccaacat
    50761 tgacactgat tactgatgac agtcactgga cactgcgttt cccgcatgac tggtttagtc
    50821 agaatgcgct ggtactgctt gatctggaaa aggagcaaga atactgggaa ggcgtggctg
    50881 gctggcggtt gaaaattgaa gaagaaagta ccccagaaat cgccgcttaa tgctttgcgg
    50941 gccgacgaga aatgtcggcc cgcattattc aggcactttc gcgaatgggt tcgatttcat
    51001 tcagcgtatc aattaacggt tgcggcttac caataagata accctgcata taatcgatcc
    51061 ccaaagagag caccgcctcg cggatctctt cgttttcaac gtactctgcc actacccgca
    51121 ttttcttcat tcgcgccaga tggcaaatcg acgccacaat ctgataatcc agactatttg
    51181 acacaatatt gcggataaag ctgccgtcaa ttttaagcag atcggcattc acatttttaa
    51241 gccgtgcata gctggcgtag ccggtgccga aatcatcaat cgcaatctgg cagcctaatt
    51301 cctgaagatg ctgcaaggtg atttgcgcct gcttaacatt ggtcagagca ttactttcgg
    51361 tgacttcaaa aataagttgc cacgcttcaa tctgatattt agccagcaac tgactgactt
    51421 caacaggaaa acgagcctga cataccgagg ttggagacag attaatagca aaacggtgag
    51481 cgggcatctt cgctctgttt tcagccataa attgcagcgt atgctcaatg acccacatgt
    51541 cgatactcga cgataaacca aattcgtgcg cgaccggcaa gaagctaccg gggccgatca
    51601 gttcatcatt ctcacctttc atgcgcagaa gaatttcatg gtaaacatca ccacgcatac
    51661 cggtaatcgg ctgggccatc aggaaaaaat ggttgtgttc cagcgcctgc tgtagtcgat
    51721 tcatcatcgc gactttatct ttcaattcgc gttgcaaata cattgccccg caacgctgca
    51781 tattttccgg ggcgttggtc acgatggaaa gttcggcgac cgtatttagc tctcccagca
    51841 gcaggtagat atgattcact ggcgagcgca catagcagta actgacgcca atctgcggtt
    51901 gcatcggcat cccatcccaa aagaaacgaa attgcttgag atgactatcc agtgcggtaa
    51961 tgcgctcctg gtgcgattct gtattcagtc gcagcgcgag atcgttaccc gaaagctgat
    52021 aaacatcttc acccggttcc agcaagggtg acagccagtg agaaagtttt tgcttgt
//
LOCUS       DAJTQY010000019        49161 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.019, whole genome shotgun sequence.
ACCESSION   DAJTQY010000019 DAJTQY010000000
VERSION     DAJTQY010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49161
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.019"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(<1..1156)
                     /gene="setB"
                     /locus_tag="OO349_001134"
     CDS             complement(<1..1156)
                     /gene="setB"
                     /locus_tag="OO349_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HCR5866290.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPAILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLF"
     gene            1524..2654
                     /gene="fruB"
                     /locus_tag="OO349_001135"
     CDS             1524..2654
                     /gene="fruB"
                     /locus_tag="OO349_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HCR5866291.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDKHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEARAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            2654..3592
                     /gene="fruK"
                     /locus_tag="OO349_001136"
     CDS             2654..3592
                     /gene="fruK"
                     /locus_tag="OO349_001136"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HCR5866292.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            3609..5300
                     /gene="fruA"
                     /locus_tag="OO349_001137"
     CDS             3609..5300
                     /gene="fruA"
                     /locus_tag="OO349_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HCR5866293.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVTATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTSQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            5724..6665
                     /locus_tag="OO349_001138"
     CDS             5724..6665
                     /locus_tag="OO349_001138"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HCR5866294.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSARKCVKVRDRLNQIHTLKPNRLEAETLSGIALSGRDDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGENGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENTECLN"
     gene            6653..7591
                     /gene="psuG"
                     /locus_tag="OO349_001139"
     CDS             6653..7591
                     /gene="psuG"
                     /locus_tag="OO349_001139"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HCR5866295.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEKIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSSFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            7685..8935
                     /locus_tag="OO349_001140"
     CDS             7685..8935
                     /locus_tag="OO349_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCR5866296.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRVNRNELFTAICSGMASIAGSVMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTVAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(9006..9700)
                     /gene="yeiL"
                     /locus_tag="OO349_001141"
                     /pseudo
     CDS             complement(9006..9700)
                     /gene="yeiL"
                     /locus_tag="OO349_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
     gene            9831..10772
                     /gene="rihB"
                     /locus_tag="OO349_001142"
                     /pseudo
     CDS             9831..10772
                     /gene="rihB"
                     /locus_tag="OO349_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708059.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
     gene            10872..12122
                     /locus_tag="OO349_001143"
     CDS             10872..12122
                     /locus_tag="OO349_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCR5866297.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEADATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(12178..13266)
                     /locus_tag="OO349_001144"
     CDS             complement(12178..13266)
                     /locus_tag="OO349_001144"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCR5866298.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKDKGYILTEQEYCVVVGTINMDIRGMADIHYPQAASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDQT
                     VLAINDTHLLEQLTPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLADEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTVPAHTTVDSFGADDGFMAGLVYSFLEGNNFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(13269..14126)
                     /gene="nfo"
                     /locus_tag="OO349_001145"
     CDS             complement(13269..14126)
                     /gene="nfo"
                     /locus_tag="OO349_001145"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HCR5866299.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLHTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQNDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(14200..15249)
                     /locus_tag="OO349_001146"
     CDS             complement(14200..15249)
                     /locus_tag="OO349_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HCR5866300.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            15348..16229
                     /locus_tag="OO349_001147"
     CDS             15348..16229
                     /locus_tag="OO349_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5866301.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DEQVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTRDIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            16435..17904
                     /gene="lysP"
                     /locus_tag="OO349_001148"
     CDS             16435..17904
                     /gene="lysP"
                     /locus_tag="OO349_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HCR5866302.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            18196..20187
                     /gene="cirA"
                     /locus_tag="OO349_001149"
     CDS             18196..20187
                     /gene="cirA"
                     /locus_tag="OO349_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HCR5866303.1"
                     /translation="MFRLNPFVRVGLCLSAISCAWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YCVSHNGRWDYGTSELKSYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYMVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGGESSVIVFRNDVKDRISISRTSDVNAAPGYQNFVGFET
                     GANGRRIPVFSYYSVNKARIQGVETELKIPFNDEWKLSINYTYNDGRDVSNGENKPLS
                     DLPFHTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVT
                     KDVKLRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(20219..21054)
                     /gene="yeiG"
                     /locus_tag="OO349_001150"
                     /pseudo
     CDS             complement(20219..21054)
                     /gene="yeiG"
                     /locus_tag="OO349_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
     gene            21312..21980
                     /gene="folE"
                     /locus_tag="OO349_001151"
     CDS             21312..21980
                     /gene="folE"
                     /locus_tag="OO349_001151"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HCR5866304.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            21997..23073
                     /locus_tag="OO349_001152"
     CDS             21997..23073
                     /locus_tag="OO349_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HCR5866305.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYDAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRLWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLGVLLLLGLISDSQTSRAWTPDASAILYEKYWKLHGGVEAISNRA
                     DGVGNSLLALGAQYGWQLAGMMLIGAALMRSGWLKGQFSLRHYRRTGFVLVAIGVTIN
                     LPAIALQWQLDWAYRWCAFLLQMPRELSAPFQAIGYASLFYGFWPQLSRFKLVLAIAC
                     VGRMALTNYLLQTLICTTLFYHLGLFMHFDRLELLAFVIPVWLANILFSVIWLRYFRQ
                     GPVEWLWRQLTLRAAGPAISKTSR"
     gene            23215..24255
                     /gene="galS"
                     /locus_tag="OO349_001153"
     CDS             23215..24255
                     /gene="galS"
                     /locus_tag="OO349_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HCR5866306.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            24535..25533
                     /gene="mglB"
                     /locus_tag="OO349_001154"
     CDS             24535..25533
                     /gene="mglB"
                     /locus_tag="OO349_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HCR5866307.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            25594..27114
                     /gene="mglA"
                     /locus_tag="OO349_001155"
     CDS             25594..27114
                     /gene="mglA"
                     /locus_tag="OO349_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HCR5866308.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            27130..28140
                     /gene="mglC"
                     /locus_tag="OO349_001156"
     CDS             27130..28140
                     /gene="mglC"
                     /locus_tag="OO349_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HCR5866309.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKISGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(28176..29389)
                     /gene="preA"
                     /locus_tag="OO349_001157"
                     /pseudo
     CDS             complement(28176..29389)
                     /gene="preA"
                     /locus_tag="OO349_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
     gene            complement(29383..30621)
                     /gene="preT"
                     /locus_tag="OO349_001158"
     CDS             complement(29383..30621)
                     /gene="preT"
                     /locus_tag="OO349_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HCR5866310.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLQRVRQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLSGELT
                     MAADKIILAVGQHARLDAFAELEQQRNTIKTQNYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            complement(30815..31054)
                     /locus_tag="OO349_001159"
     CDS             complement(30815..31054)
                     /locus_tag="OO349_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HCR5866311.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(31057..31776)
                     /gene="sanA"
                     /locus_tag="OO349_001160"
     CDS             complement(31057..31776)
                     /gene="sanA"
                     /locus_tag="OO349_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HCR5866312.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(31926..32810)
                     /gene="cdd"
                     /locus_tag="OO349_001161"
     CDS             complement(31926..32810)
                     /gene="cdd"
                     /locus_tag="OO349_001161"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HCR5866313.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(32940..33635)
                     /locus_tag="OO349_001162"
     CDS             complement(32940..33635)
                     /locus_tag="OO349_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HCR5866314.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(33632..34030)
                     /locus_tag="OO349_001163"
     CDS             complement(33632..34030)
                     /locus_tag="OO349_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HCR5866315.1"
                     /translation="MSKTLNIIWQYLRAFILIYACLYAGIFIASLLPVTIPGSIIGMQ
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            34269..35216
                     /gene="dusC"
                     /locus_tag="OO349_001164"
     CDS             34269..35216
                     /gene="dusC"
                     /locus_tag="OO349_001164"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HCR5866316.1"
                     /translation="MRVLLAPMEGVLDSQVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGFWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISDC
                     DAVMIGRGAFNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
     gene            complement(35587..35670)
                     /gene="yohP"
                     /locus_tag="OO349_001165"
     CDS             complement(35587..35670)
                     /gene="yohP"
                     /locus_tag="OO349_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohP"
                     /protein_id="HCR5866317.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            35894..37330
                     /gene="mdtQ"
                     /locus_tag="OO349_001166"
     CDS             35894..37330
                     /gene="mdtQ"
                     /locus_tag="OO349_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HCR5866318.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAVATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVDGVETDINASKTRQQLNDVAGKMKIIEARLNALTNHQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIRLTGALGGGYKR"
     gene            37383..38144
                     /locus_tag="OO349_001167"
                     /pseudo
     CDS             37383..38144
                     /locus_tag="OO349_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416641.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(38261..38839)
                     /locus_tag="OO349_001168"
     CDS             complement(38261..38839)
                     /locus_tag="OO349_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5866319.1"
                     /translation="MALNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            39009..39596
                     /locus_tag="OO349_001169"
     CDS             39009..39596
                     /locus_tag="OO349_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HCR5866320.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLTLTVILWGYGYRLF"
     gene            39770..40702
                     /gene="pbpG"
                     /locus_tag="OO349_001170"
     CDS             39770..40702
                     /gene="pbpG"
                     /locus_tag="OO349_001170"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HCR5866321.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(40740..42455)
                     /gene="dld"
                     /locus_tag="OO349_001171"
     CDS             complement(40740..42455)
                     /gene="dld"
                     /locus_tag="OO349_001171"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCR5866322.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARIDEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            42651..44948
                     /gene="bglX"
                     /locus_tag="OO349_001172"
     CDS             42651..44948
                     /gene="bglX"
                     /locus_tag="OO349_001172"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HCR5866323.1"
                     /translation="MKWLCSVGIAVSLVLQPALADDLFGNHPLTPEARDAFVTDLLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMAELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNSV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQGQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPISFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            45200..46117
                     /gene="osmF"
                     /locus_tag="OO349_001173"
     CDS             45200..46117
                     /gene="osmF"
                     /locus_tag="OO349_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HCR5866324.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     FQVLESHGVPTVNKVQLGTTPVGRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAG
                     QGYEKVKKLDAEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPTFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWAK"
     gene            46124..47281
                     /gene="yehY"
                     /locus_tag="OO349_001174"
     CDS             46124..47281
                     /gene="yehY"
                     /locus_tag="OO349_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HCR5866325.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGLHLWQL
                     WPQTIWMLVGVGCAWLTACFVPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGRGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALLVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            47274..48200
                     /gene="yehX"
                     /locus_tag="OO349_001175"
     CDS             47274..48200
                     /gene="yehX"
                     /locus_tag="OO349_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HCR5866326.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVHLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLVEA"
     gene            48205..48936
                     /gene="yehW"
                     /locus_tag="OO349_001176"
     CDS             48205..48936
                     /gene="yehW"
                     /locus_tag="OO349_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HCR5866327.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPILQVTLAGLGAIDPSVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(48917..49024)
                     /locus_tag="OO349_001177"
     CDS             complement(48917..49024)
                     /locus_tag="OO349_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HCR5866328.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(49084..>49161)
                     /locus_tag="OO349_001178"
     CDS             complement(49084..>49161)
                     /locus_tag="OO349_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR5866329.1"
                     /translation="NRTSAQQQQLTSWQEQGHDIFPLGI"
BASE COUNT        11876 a        12101 c        13201 g        11983 t
ORIGIN      
        1 aaaacagagt ggcgataatc atcaccatcg caaaccagaa cacagcgtga taattccaga
       61 tctcggcgac gatgcccgcc actgatcctg cgatgatcca gcccacgcgc gacgtgttgg
      121 tatagagcgt ggtggctgaa cctgcctgac cgggcatcaa atcctgaaaa tagagcatcc
      181 cgatgccgcc cagaatgcca ataaaaatag catttagcag ctgcaagccc aacagaatgg
      241 caggtgaatg cgccatcagc attcctgcgt aaaaacagac gccacccacg gcagcaacgc
      301 gcattaagaa acgcttaccc agacgtttgg cgaaatatcc ggcaatcaac atcgtcggga
      361 tttccagccc ggcggcggtc cccatcatca caccggctag tttctcggga agatgcagtt
      421 cgttgataat aaatagcggc atgttgatga tgtacaggct gttcgagccc cacatcaatg
      481 tacaaatgac aaacagcagt agcgtatcgc gacggttacg gcgcggcgct tcaaccgtgc
      541 cggtcgccag cggaagctct ttttgcatcg acggtaaaaa cagccacacc atcacgccgc
      601 aaacaataaa cgctaccgct gcgctcagat acattaccgt aaagctgaaa cccatcgcta
      661 acgcataagc cagcggtggg ccaatgaccc atgccagtga aacctgagcg cgtaaaaaag
      721 agctgaacat caccgcctca cgtccggttt tgtcggcatg ttcacgggca agggcaaaca
      781 tttgcgggtt agcggtcgag ccaaagctgc taagaaagac gccaacgaat agcaaaacaa
      841 agtagttgcg attccaagca aaaagggtgc aggccagcac gcctaacagg cagcaaaaga
      901 caatcagcga tttgcgatcg ccgcgcttat cagagcgccc agcgagaaac tgacttacca
      961 gaatcccaat gacagcgctg ccggtaaaga agaatcccac catcgccgga cgggcatgta
     1021 cttcatcggt aaggaaaata ctgagtgtcg gggtttgcag agcacccgca ataccggtga
     1081 gaaaggcaac gattaaaaac gccgtcgagg tcaggtcaaa cgatttcgcg ctggagactg
     1141 cgggggagtt atgcatgctg gtcgatcact ggaagaggaa gacgcggagt ttacgctgct
     1201 tgtcagaaaa taaacagaca gcgaaatcaa aagcgccacg aataatcaaa atgccatttc
     1261 actgttgctg attggctgct gacgctgctg aaatgaaatt gctgataacg gattttccca
     1321 tcagcaatta ggaaaaatga caaaaaattg tgcagcacat caaacttttg ctcataactt
     1381 tacggctttc cttgcgtgct gaaacagaag tattatgctt tcttgaaacg tttcagcgcg
     1441 atcttgtctt taaccctaag ctaggttggc gctttttttc tcatagaggc tgaatcgttt
     1501 caattcagca agagaggaga actatgttcc agttatccgt acaggacatc catccgggcg
     1561 aaaaggccgg agacaaagaa gaggcgattc gccaggtcgc tgcggcgctg gtgcaggccg
     1621 gtaatgtagc agaaggctac gtcaatggca tgctggcgcg cgaacagcaa acctcaacgt
     1681 tcctcggcaa tggtattgct attccacacg gcactaccga cacccgcgat caggtgctga
     1741 aaaccggcgt tcaggtattt cagttcccgg aaggcgtcac ctggggtgac ggtcaggtag
     1801 cgtacgtggc aatcggtatt gctgccagct cggataaaca tctgggcctg ctacgccagc
     1861 tgacccacgt actgagcgat gattccgttg ctgaacaact gaagtcagca acaacagcag
     1921 aagaacttcg cgcattactg atgggcgaaa agcagagtga gcagctgaag ctcgacaacg
     1981 aaatgctgac gctggatatc gtcgccagcg atctgctgac tcttcaggcg ctgaacgctg
     2041 cgcgtctgaa agaggcgagg gcagttgacg ccactttcgt caccaaagcc atcaatgaac
     2101 aaccgctgaa tctcggacag ggtatctggc tgagcgatag cgccgaaggc aatctgcgta
     2161 gtgcgattgc ggtaagccgt gcggcaaatg cttttgatgt ggacggcgaa acggcagcca
     2221 tgctggtgag tgtggcgatg aatgacgatc agcccatcgc ggttcttaag cgtctcgctg
     2281 atttgttgct cgacaataaa gctgaccgct tgctgaaagc ggatgcggca acgttgctgg
     2341 cgctgctgac cagcgatgat gcgccgaccg acgacgtgtt aagcgcggag tttgtagtgc
     2401 gcaatgaaca cggcctgcat gctcgtccag gtaccatgct ggtcaatacc attaaacaat
     2461 ttaacagtga tattaccgtg acaaaccttg atggcaccgg caaaccggca aacggacgta
     2521 gtctgatgaa agttgtggca cttggcgtta agaaaggtca tcgcctacgc tttaccgccc
     2581 agggtgcaga tgctgaacag gcgctgaaag ccattggcga cgctatcgct gctggtcttg
     2641 gggagggcgc ataatgagca gacgtgttgc tacgatcacc cttaatccgg cttatgacct
     2701 tgttggtttc tgcccggaaa ttgaacgcgg cgaagtgaac ctggtgaaaa ccaccggtct
     2761 gcatgcggcg ggtaaaggca tcaacgtggc caaagtatta aaagacctgg gaattgatgt
     2821 caccgttggc ggcttcctgg gtaaagacaa tcaggatggt tttcagcaac tgttcagcga
     2881 gctgggcatt gccaaccgtt tccaggttgt acaggggcgc acccgaatta acgttaagct
     2941 gacggaaaaa gacggcgaag tgaccgactt caacttctcg ggtttcgaag tcacccccgc
     3001 cgactgggaa cgctttgtga ctgattctct gagctggctc ggtcagttcg atatggtctg
     3061 tgtcagcgga agcttaccgt caggcgtcag cccggaagcg ttcaccgact ggatgactcg
     3121 cctgcgtagt cagtgtcctt gcattatctt tgatagtagc cgtgaagcgt tagtagcagg
     3181 tttgaaagcg gcaccgtggc tggtgaaacc taaccgccgc gagttggaaa tctgggcagg
     3241 ccgtaaactg cctgaaatga aagatgtgat tgaagctgca catgcgctac gtgaacaagg
     3301 tattgcgcat gttgttattt cactgggtgc cgaaggcgcg ctttgggtta atgcctccgg
     3361 cgaatggatc gctaaaccac cgtcagtcga tgtcgtaagc accgttggcg caggggattc
     3421 tatggttggt ggcctgattt atggcttgct gatgcgtgaa tccagtgaac acacactgcg
     3481 tctggcgaca gctgttgcag ccctggcggt aagtcaaagc aatgtgggta ttaccgatcg
     3541 tccgcagttg gccgcaatga tggcgcgcgt cgacttacaa ccttttaact gacagcagga
     3601 gaggcataat gaaaacgctg ctgattattg acgctaatct cggtcaggca cgcgcctata
     3661 tggcgaagac cctgctgggc gcggcggcgc gaaaagcaaa actggaaatc atcgacaatc
     3721 cgaacgacgc agaaatggcg attgttctcg gtgattccat cccgaatgac agcgcgctga
     3781 acggtaaaaa tgtctggctg ggtgatattt cccgggcagt tgcgcaccct gagctgttcc
     3841 tgagtgaagc caaaggccat gcgaaacctt acactgcgcc ggtcactgcg acagcaccgg
     3901 ttgccgccag cggtccgaaa cgcgtagttg cggtgactgc ttgcccgact ggcgtagcac
     3961 acacctttat ggcggctgaa gccattgaaa ccgaagcgaa aaaacgtggc tggtgggtga
     4021 aagttgaaac ccgtggttct gttggcgcgg gtaatgcaat cactcccgag gaagtagccg
     4081 cagcggatct ggtgattgtg gcggcagata tcgaagtgga tctggcgaaa tttgctggta
     4141 aaccgatgta tcgtacctct accggtctgg cgctgaagaa aacctcgcag gaactggata
     4201 aagcggttgc tgaagcaacg ccgtatgaac cggcgggcaa agctcaaacg gcgaccactg
     4261 aaggtaagaa agagagtgca ggcgcttatc gtcacttgct aacgggcgtc tcttacatgc
     4321 tgccgatggt cgttgctggt ggtctgtgta tcgcgctttc ttttgctttt ggtatcgaag
     4381 cgtttaaaga gccgggtacg ttggcagcgg cgctgatgca gattggtggt ggttcagcct
     4441 ttgcgctgat ggtgccggta ctggcaggtt atattgcctt ctccattgcc gatcgtccgg
     4501 gcctcacgcc gggtctgatt ggcggtatgc tggcggtcag caccggttct ggcttcattg
     4561 gcggtattat tgcgggcttc ctggctggtt acattgcgaa gttaatcagt acgcaactga
     4621 aactgccgca gagtatggag gcgctgaaac cgatcctgat cattccgcta atttccagtc
     4681 tggtggtcgg tctggcgatg atctacctga tcggtaaacc agttgctggc attctcgaag
     4741 ggctgactca ctggctgcag accatgggga ctgcgaatgc ggttctgctg ggggcgatcc
     4801 tcggtggcat gatgtgtact gacatgggcg gtccggtaaa caaagcagcg tacgcattcg
     4861 gtgtgggtct gctgagtact caaacctatg gcccgatggc ggcgattatg gcggcaggta
     4921 tggtgccacc gctggcaatg ggcctggcaa caatggtggc gcgtcgcaaa ttcgacaaag
     4981 cgcagcagga aggtggcaaa gccgctctgg tattgggact gtgcttcatt tcggaaggtg
     5041 caattccgtt tgctgctcgt gatccgatgc gtgtgctgcc gtgctgtatc gtgggtgggg
     5101 cgctgactgg cgcaatctca atggcgattg gtgcgaaact gatggcaccg cacggtggtc
     5161 tgtttgttct gctgatccct ggcgctatta cgccggtatt gggttacctg gtagcaatta
     5221 ttgccggtac gctggtggcg ggtttggcct atgccttcct gaaacgtccg gaagtggacg
     5281 cagtagcgaa agcagcgtaa taaaaggtgt gttacagggc agaaatttcc tgccctgtaa
     5341 catttgatga gcgcaaagag aatgaccagc ttgtgaatgt aattttatga atttaaatgg
     5401 ttttatcttt cagtagaaac ttcaaacgtt catcaaacac taaggttatt tcctcaaggg
     5461 cagattattt tctgcccttt tttagtgttc ctgttctgtg catcccgtca caaattcctc
     5521 gcttttgtaa ttcaggcatt taatcgcatt ttgtgagcct gctcaaatct gcccattttg
     5581 cctctctggc ggtaatttac tttacgtaaa acatgcatac tatgagcaca tgtttaaata
     5641 tgaacacatg tttaaaccgt ctttgcagta tctatattct tgtggattga cagggttggt
     5701 aattttttac cagaggacgt gctatgcgcg aaaaggatta tgtcgtaatt ataggttcgg
     5761 cgaatattga tgtcgccgga tattcacatg aatcattaaa ttatgcggat tcaaatccag
     5821 gtaaaataaa atttacgcct ggtggagtag ggcgcaatat tgcacaaaac ctggcgttgc
     5881 tgggtaacaa agcctggcta ctgagcgccg taggcagtga tttttatggt caatcgctgc
     5941 taacgcaaac caatcaatct ggtgtttatg tcgataaatg cctgattgtg ccgggagaaa
     6001 atacgtcgag ttatttatca ttactcgata ataccggtga aatgctggtt gctataaatg
     6061 acatgaatat tagcaacgct attacagctg aatatctcgc acagcaccgt gaatttattc
     6121 agagggcaaa ggtcattgtc gcggactgta atatcagtga agaggcactg gcatggattc
     6181 tggataatgc cgccaacgta cccgtatttg tcgatccggt ttcagcgcgg aaatgtgtca
     6241 aagtgcgcga ccgtctaaat cagatccaca ctctgaagcc aaaccgcctt gaagcggaaa
     6301 ccctgagtgg gattgcgctg tcagggcgtg acgatgtggc aaaagttgct gcctggttcc
     6361 atcaacatgg cctgaaccga ctggtattga gcatgggcgg cgacggcgtt tattacagcg
     6421 atatcagcgg tgaaaatggc tggtctgcgc cgatcaaaac caatgttatt aatgttaccg
     6481 gagcgggcga tgccatgatg gcgggacttg cttcgtgttg ggtagacgga atgccgtttg
     6541 ccgaatctgt tcgtttcgca cagggatgtt cgtcaatggc gctctcctgt gaatacacca
     6601 ataaccccga tttatcgatt gccaacgtta tatcgttagt ggagaacaca gaatgtctga
     6661 attaaaaatc tcccctgaat tattacaaat ttctccggaa gtgcaggacg ctttaaaaaa
     6721 caaaaaaccg gttgtggcgc tggaatcgac cattatttct cacgggatgc cgttcccaca
     6781 aaatgcccag accgcaattg aagtagaaga aactattcgt aaacagggcg cagtacctgc
     6841 cactatcgcc attattggcg gcgtgatgaa agtgggttta agcaaagaaa aaattgaatt
     6901 actgggtcgt gaagggcata acgtgaccaa agttagtcgt cgcgatttac cttttgttgt
     6961 tgccgccgga aaaaatggcg caaccactgt ggcttcaacg atgattattg cggcgcttgc
     7021 cggaattaaa gtatttgcca ccgggggaat tggtggtgtg catcgcgggg cggaacatac
     7081 cttcgatatt tctgccgatt tgcaagaact ggcaaatact aatgtcaccg ttgtttgtgc
     7141 cggggcgaaa tctattctcg atttaggatt aaccactgag tatttagaaa ccttcggtgt
     7201 gccgttaatt ggctatcaga ctaaagcgct gcctgcgttt ttctgccgta ccagctcgtt
     7261 tgacgtcagc attcgtctcg acagcgccag tgaaattgcc cgtgcaatgg cggtgaaatg
     7321 gcaaagcggg ctgaacggtg gcctcgtggt agcgaacccg atcccggaac agtttgcgat
     7381 gccggaggaa tctatcaatg cagccataga tcaagccgtc gccgaagccg aagagcaggg
     7441 cgttattggt aaagaaagta caccgttcct gctggcgcgc gttgctgagc tgaccggcgg
     7501 tgacagcctg aaatccaata tccagctggt gttcaacaac gccattctgg cgagtgaaat
     7561 tgccaaagaa taccagcgtc tcgcgggtta atttctgacg ggcagaaagc cgtcgcccgt
     7621 actcatcaag cataccaaaa cctggcgctg acgccgggtt tactggcatg aagggaaaat
     7681 cattatggat ataatgagaa gtgttgtggg gatggtggtg ttactggcaa tagcatttct
     7741 gttgtcagtg aataaaaaga gcatcagttt gcgcacggtt ggagccgcac tgctgctgca
     7801 aatcgctatt ggtggcatca tgctctactt cccaccggga aaatgggcag tagaacaggc
     7861 ggcattaggc gttcataaag tgatgtctta cagtgatgcc ggtagcgcct tcatttttgg
     7921 ttcgctggtt gggccgaaaa tggatgtcct gtttgacggt gcgggtttta tcttcgcctt
     7981 tcgcgtactt ccggcgatta ttttcgttac tgcgctcatc agtctgctgt actacattgg
     8041 cgtgatgggg ctgctgattc gcatccttgg cagcattttc cagaaagccc tcaacatcag
     8101 caaaatcgaa tcttttgttg cggttaccac tattttcctc gggcaaaatg agatcccggc
     8161 gatcgttaaa ccgtttatcg atcgcgtgaa tcgcaacgag ttgtttaccg caatttgtag
     8221 cgggatggcg tccattgctg gttcggtgat gattggttat gccggaatgg gcgtaccaat
     8281 tgactacctg ttagcggcat cgctgatggc gatccccggc ggtattttgt ttgcacgtat
     8341 tcttagcccg gcaaccgagc cttcgcaggt cacatttgaa aatctgtcgt tcagcgaaac
     8401 gccgccaaaa agctttatcg aagcggcggc gagcggtgcg atgaccgggc tgaaaatcgc
     8461 cgctggtgta gcgacggtgg taatggcgtt tgtcgcaatt attgcgctga tcaacggcat
     8521 tatcggcgga attggcggct ggtttggttt cgccaatgcc tctctggaaa gtatttttgg
     8581 ctatgtgctg gcaccgctgg cgtggatcat gggtgtggac tggagtgatg ccaaccttgc
     8641 tggtagcctg attgggcaga aactggcgat taacgaattc gtcgcttacc tgagtttctc
     8701 cccatacctg caaacgggcg gcacgctgga agtgaaaacc gtggcgatta tatcctttgc
     8761 gctttgtggt tttgctaact ttggttctat cggtgttgtc gttggcgcat tttcggctat
     8821 ttcgccaaaa cgtgctccag aaatcgccca gcttggttta cgggcgctgg cagcagcaac
     8881 gctttccaac ctgatgagtg cgactattgc cgggttcttt attggtctgg cgtaatgctc
     8941 gatttgtaga ccggataaaa cgtattaagc gttgcatccg gcaatcgcgc tattgcaaat
     9001 aatttttact gcatcatccc ggagaattta ttctccgggt ccatctccag cgccagtcct
     9061 gacaactgct ttctgttttt aattagatat cctttctttt aattaataaa ccgtcgtgaa
     9121 tgaattgcgc gagaacatat aaaagatgtc gataagaaac gcctaaatac tctgccgctt
     9181 gcgtatgctt ttcgtgataa agatcacctt cttgtgagag taatataaat gctgccaggc
     9241 gattcactaa cggaaatgat tgattctgag ttaaagaaac aatattacga taatttttat
     9301 gacttaaggt gacgcaaagt tttcgtaaaa atagggtgtc gtttaataac agcggacggt
     9361 aatgtttcat agggagcgca aggcaccaac actcttcaat agcctgcacc gcacgcggtt
     9421 catggtcttt gtcgattaac tcaatctcgc caataaaaca gggagcggca aagaaatcga
     9481 tcagcgacac gcgaccattg gcaagcgtgg cgtaaagcct ggcgcggcct cgcgtcagat
     9541 aaaacagcca tgacggctgt tgcccttcct gcacaatgta gtcacgcgct aaaaaatgaa
     9601 acagccgcgt atcggctgaa acatccgttg aaaagcaatc cttaaacgca gattcactca
     9661 tcaattgctg cttaaggtca ttattgtgga tttctttcac gatgtttatc cgctatgaga
     9721 tttctcatat ttagcatacg tacgctgttg ttaagattca atcattggcc cggtcgaatt
     9781 ttctttgctg tagaaaaaaa taacgcaact ggaaacagag gaaataaaca atggaaaaga
     9841 gaaaaattat tctggattgt gagccgggtc atgatgatgc tattgcaatg atgatggcag
     9901 cgaaacatcc ggcaatagat ttattaggca tcactattgt agcgggtaat cagacgcttg
     9961 ataaaacatt aattaatggc ctgaatgttt gccagaaact ggagattaat gttccggttt
    10021 atgcggggat gccgcaaccc attatgcgta aacaaatcgt tgccgataat attcacggtg
    10081 aaaccggact ggatggcccg gtattcgagc cgctgacccg ctaggcagaa agtacccatg
    10141 cggtgaaata tatcatcgat accctgatgg caagcgatgg cgatatcact ctggtgccgg
    10201 ttggtccgct ttcaaatatc gcggtggcaa tgcgtatgca acccgcaatc ctgcccaaaa
    10261 tccgtgaaat tgtgctaatg ggcggcgctt acggtacagg caacttcacc ccatctgccg
    10321 agttcaatat ctttgccgac ccggaagccg cccgcgtggt gttcacctcc ggcgttccat
    10381 tagtgatgat gggcctcgat cttaccaacc agaccgtttg caccccggat gtaattgctc
    10441 ggatggaaag ggtaggcggc cctgccggag agctgttcag cgacatcatg aacttcactc
    10501 tcaaaacgca gttcgaatac tacggcctgg ctggcggccc ggtgcatgac gccacctgca
    10561 ttggttatct gatcaaccct gatggcatta aaacccagga tatgtacgtc gaagtggacg
    10621 tcaacagcgg cccttgctac gggcgtacgg tctgcgacga actgggcgtt cttggcaagc
    10681 ccgctaatac caaagtcggc atcactattg ataccgactg gttctggggc ttagtcgaag
    10741 agtgcgtgcg cggctacatc aaaacccatt aagtgttgat aacaagccgg gacagtatcg
    10801 caagatgcag gcccggctat ttctgatggg cagaaagccg tcgcccaaaa tgacatgaag
    10861 agataatcac tatggatgtc atgagaagtg ttctgggaat ggtggtactg ctggcaattg
    10921 cgtttttact gtcagtaaac aagaagaaga tcagcctgcg taccgttggc gcggcgttag
    10981 tgttacaggt cgtgattggc gggattatgc tttggttacc gccagggcgt tgggtcgcag
    11041 aaaaagtcgc ttttggcgtc cacaaagtga tggcgtacag cgacgcaggt agcgcattta
    11101 tcttcggttc tctggtcgga ccgaaaatgg ataccttatt tgatggcgca ggatttatct
    11161 ttggtttcag ggtattaccg gcaattatct tcgtcactgc actggtgagt attctctact
    11221 acatcggtgt gatggggatc ttaattcgca ttctcggcgg tatcttccag aaagctttaa
    11281 atatcagcaa gatcgagtca ttcgtcgcgg tcaccaccat tttcctcggg caaaacgaaa
    11341 ttccggcgat cgtcaaaccc tttatcgatc gtctgaatcg caatgaatta tttacagcga
    11401 tttgtagtgg catggcctcg attgctggtt cgacaatgat tggttacgcc gcactgggcg
    11461 tacctgtgga atatttgctg gcggcatcgt taatggcgat ccctggcggg atcttgtttg
    11521 cccgcctgtt aagcccggct acggaatctt cgcaggtttc ttttaataac ctctctttca
    11581 ccgaaacacc gccaaaaagc attattgaag ccgatgcgac aggggcaatg accgggctga
    11641 aaatcgccgc cggtgtagcg acagttgtta tggcatttgt cgccatcatt gcgttaatta
    11701 acggtattat cggcggcgtt ggcggctggt ttggttttga acatgcctcg ctggagtcca
    11761 ttttaggtta cctgctggcc ccacttgcgt gggtaatggg tgttgactgg agtgatgcaa
    11821 atcttgccgg gagtttgatt ggacagaaac tggcaataaa tgaatttgtc gcttatctca
    11881 atttctcacc ctatctgcaa acggctggca ctctggatgc taaaaccgtg gcgattattt
    11941 ccttcgcgtt gtgcggtttc gctaactttg gttctatcgg ggtggtggtg ggggcgtttt
    12001 ctgcggttgc gccacaccgt gcgccggaaa tcgcccagct tggtttacgc gcgctggcgg
    12061 cggcgacact ttctaatctg atgagtgcca ccattgccgg gttctttatt ggtttagcgt
    12121 agtgtgcaat ttatgagccg gattagatgc ggtcaagcgt cgaatccggc aacattgtta
    12181 caccattggc actaatgaca gcgcgttatc ggcagacagg gtagggttgt ttaagctgcc
    12241 gctggcgcgt gaaattgccg cgcaggccat cgcaaaacgg gcgctgtcac ggaaattgtt
    12301 tccctccaga aagctatata ccagacccgc cataaaacca tcgtcagcac caaaactgtc
    12361 taccgtcgta tgcgctggca cagtcagcaa aaattgttca ccatcctttt cgctgcaata
    12421 aactgactca tcgggcaaat aaacaaacag ttgctgaaca ccttgctgat gcaatgcatt
    12481 cactgcggca ttacggtcag catcgctggt gatcgcctgt ccccataaaa tttccagctc
    12541 cggtaaagtg ggtttcaggg tgtgaatatg cgccagccag tgtttgattt tgcccgcttt
    12601 gaattctgaa acggtatcga caaacaccgg gatctcatca gcgagggtaa agacccattc
    12661 cagcgcctcg gctgtcaggt tgcaatcagc cagtaccacg cccgcatgac gaagtaaatc
    12721 gcgcgacccg ttcagtagtt gcggtgtcaa ctgttccagc agatgggtat cgttaattgc
    12781 cagcacggtt tgatcgtctc gattggcaat tgccagatac gtcgatgtgc tttgaccatg
    12841 caaacgaacg cagccggaga cattcacgcc ggcacggcgc gtttcttcca ggagcatttc
    12901 gccataaaag tcatcgccaa tcactgaaag caaatggacg tcacggccta acagcgccag
    12961 attgtgggcg atattgcgtc caacgccgcc tgccgaacaa tgaattgtac cgggatgaga
    13021 agccgcttgc gggtaatgga tatccgccat cccgcgaata tccatattga ttgtccccac
    13081 cactacgcag tattcctgct cggtgagaat gtaacctttg tctttaatcc ggcctttgcg
    13141 cattaaatcc ataatatgcg ccgcaacgcg cgaacgactg atttgcagca tgtccgcaat
    13201 ttcgttctgc tgaatcagcg ggttacgccg taaaattgca aggatctcct tttcccggtt
    13261 attcatcttc aggctaccgc tttttcagtt tgttgcgctt tcagccaggc gatctcttct
    13321 gcccagatat ccgggttgat ggtttcaagg atcagcggaa tgccgtcgaa acggtcgttc
    13381 tgcataatcc agcggaacgc atcatgaccg atattgcctt caccgaggct atgatggcgg
    13441 tcaacgcggc tgccaaaggt acttttcgca tcgttaaggt gcatcccgcg cagatactta
    13501 aagccgacaa tacgggcaaa atcagcgaac gttttttcgc attcggctgg agtatgcaaa
    13561 tcatacccgg cagcgaaagc atggcaggta tcaatgcaga cgccgacgcg ggatttatct
    13621 tccacgccgt cgataatcgc cgcgagatgt tcgaatttaa accctaagtt actgccctga
    13681 ccggcggtgt tttcaatcac cgctgttaca ccttgagttt tatccagcgc aatgttgatg
    13741 gattcggcaa tacgcgcaag gcaatcctct tctgaaatct gcatcagatg gctgccaggg
    13801 tggaagttga gcaaagaaag ccccagttgt tcgcaacgct gcatttcatc tataaaggca
    13861 tcgcgcgatt tttccagagc ttcagtgacc ggatgtccga ggttgatcag ataactgtcg
    13921 tggggaagaa tttgcgccga tgtgtagtga tatttttcac aggcggcttt gaattcatcg
    13981 atggtttgcg tcgtgagcgg tgcggcacgc cactgacgtt ggtttttggt gaacaaggca
    14041 aacgcggttg cgtcgatttc ggcggcgcga attgcggcat ttgccagacc gccagcagca
    14101 ctaacgtgcg ctccaatgta tttcatgcga ggactcctgt taaacccgct ggaggaaaac
    14161 ggtaatgata gcgggttaac aggagcaaga agtagtggtt tatgcgatga cgctttgaat
    14221 cacatagtta atcgcaccac caccaacaat cagccaggca aacagtacca gtgccatcag
    14281 cagaggtttc gccccagctt ttttcagcgc gctgacgtga gtggtcagac ccagcgccgc
    14341 catcgccatt gccagcagga aggtatccag cgttaccagc atgttcacca cgctctgcgg
    14401 taacaggtgg aacgagttaa agatggcaac tacgatgaac aagatggcaa accacggaat
    14461 agtgattttg cttttctcgc cgctgttcgc cccagacagc tgtttaacac gcgccgccag
    14521 caggatgagg aacggagcca gcatcatcac gcgcagcatt ttggaaataa ctgctgcgtt
    14581 ttctgcatcc gggctgatgg catgacctgc cgccaccacc tgcgccactt cgtgcacagt
    14641 agaaccaatg tagataccga aagtttccgg actaaaccat tgagacatca gcggatatat
    14701 cgccgggtag aggaaaatcg cgacggtccc gaagataaca acggttgcaa cagccacggt
    14761 tactttactg gcttccgctt tcactaccgg ctcagtcgcc agtaccgcgg cagcaccaca
    14821 gatactgcta ccggcaccga tcaaccaact ggtgtgctta tccagaccaa acactttctg
    14881 ccccaggaag caagccagca ggaaggtact ggacagcgtc agcacgtcaa tgatgatccc
    14941 actgataccg acatcggcaa tttgcgagaa cgtcagacgg aagccataaa gaatgatacc
    15001 cagacgtaat aaatattgct tggcaaacag cacaccaccg tcacagcttt tccagatgtg
    15061 cggatagatg gtgttgccta aaaccatccc caacaagatt gcgagggtga gggcactaaa
    15121 cccggcaccc gcaaccgcgg gaatggaacc accccacagg gcgaccccgg tgataactgc
    15181 actcagggct aaccccggaa taaaatgcca cagtgtacga tgttgtttct gtaaggtgat
    15241 attcgtcata accctctcct ttaaccgggc ataaggttac gactaactgg tttaaaaata
    15301 aaattgatta tatatttata attaatcttt ataagtggta agcgactatg cacatcaccc
    15361 tccggcagtt ggaagttttt gcagaagtat tgaaaagtgg atcaaccacc caggcgtcgg
    15421 tgatgctggc gttgtcgcaa tcagcagtga gcgcagcctt gactgacctg gaagggcagc
    15481 ttggcgttca actgtttgat cgcgtgggga aaagactggt tgttaatgaa cacgggcggc
    15541 tgctctatcc gcgagcgttg gcactgcttg aacaggcggt tgaaatcgaa caactgtttc
    15601 gcgaagacaa cggcgcgatt cgtatctatg ccagtagtac catcggtaac tacattctgc
    15661 ctgcagttat cgcccgttat cgccatgatt atccgcagtt gccgattgaa cttagcgttg
    15721 ggaacagcca ggacgtgatg caagcggtgc tggatttccg cgttgatatt ggctttattg
    15781 aaggaccgtg ccacagcact gaaatcattt ctgaaccgtg gctggaagac gagcaggtgg
    15841 ttttcgccgc gccgacttcg ccgttggctc gtggtccggt caccttagaa cagctggccg
    15901 ctgcgccgtg gatcctgcgt gaacgcggtt ccggcacgcg ggatattgtc gattatctgt
    15961 tgctgtcaca tttaccgaag tttgagatgg cgatggaatt aggtaactcc gaggcaatca
    16021 aacatgcggt gcgtcatggg ttgggaatta gttgcctgtc gcgacgtgtg attgaagatc
    16081 aattgcaggc aggcacatta agtgaagttg cggtccctct gccgcgcctg atgcgtacgt
    16141 tgtggcgtat acatcatcgg caaaaacacc tttccaacgc gctacggcgc tttctggact
    16201 attgcgatcc cgcaaatgtg ccgcgttaag ttgctgtaca agaacatgct ggtgctgtgt
    16261 cgatttcgtg acgcagcgcc ttcagcatgc attcgccaga aaagagattg gctgctttac
    16321 ttataatccc tgggcgatca tgaaggtgtc ttataaccgt gtatttctgc cggaaggatt
    16381 gccaatcgtc tgctacaatc gcgccatcat ttttaagatg gatagcattt ttgtatgggt
    16441 tccgaaacta aaactacaga agcgccgggc ttacgccgtg aattaaaggc gcgtcacctg
    16501 acgatgattg ccattggcgg ttccatcggt acaggtcttt ttgttgcctc tggcgcaacg
    16561 atttctcagg caggtccggg cggggcattg ctctcgtata tgctgattgg cctgatggtt
    16621 tacttcctga tgaccagtct cggtgaattg gctgcatata tgccggtttc cggttcgttt
    16681 gccacttacg gtcagaacta tgttgaagaa ggctttggct tcgcgctggg ctggaactac
    16741 tggtacaact gggcggtgac tattgccgtt gacctggttg cagcacagct ggtcatgagc
    16801 tggtggttcc cggatacacc gggctggatc tggagtgcgt tgttcctcgg cgttatcttc
    16861 ctgttgaact acatctctgt tcgcggcttt ggtgaagcgg aatactggtt ctcactgatc
    16921 aaagtcacga cagttattgt ctttatcatc gttggcgtgc tgatgattat cggtatcttc
    16981 aaaggcgcgc agcctgcggg ctggagcaac tggacaatcg gcgaagcgcc gtttgctggt
    17041 ggttttgcgg cgatgatcgg cgtagctatg attgtcggct tctctttcca gggaaccgag
    17101 ctgatcggta ttgctgcagg cgagtccgaa gatccggcga aaaacattcc acgcgcggta
    17161 cgtcaggtat tctggcgaat cctgttgttc tatgtgttcg cgatcctgat tatcagcctg
    17221 attattccgt ataccgatcc gagcctgctg cgtaacgatg ttaaagacat cagcgtcagt
    17281 ccgttcaccc tggtgttcca gcacgcgggt ctgctctctg cggcggcggt gatgaacgca
    17341 gttattctga cggcggtgct gtcagcaggt aactccggta tgtatgcgtc tactcgtatg
    17401 ctgtacaccc tggcgtgtga cggtaaagcg ccgcgcattt tcgctaaatt gtcgcgtggt
    17461 ggcgtgccgc gtaatgcgct gtatgcgacg acggtgattg ccggtttgtg cttcctgacc
    17521 tccatgtttg gcaaccagac ggtatacctg tggctgctga acacctccgg gatgacgggt
    17581 tttatcgcct ggctggggat tgccattagc cactatcgct tccgtcgcgg ttacgtattg
    17641 cagggacacg acattaacga tctgccgtac cgttcaggtt tcttcccact ggggccgatc
    17701 ttcgcattca ttctgtgtct gattatcact ttgggccaga actacgaagc gttcctgaaa
    17761 gataccattg actggggcgg cgtagcggca acgtatattg gtatcccgct gttcctgatt
    17821 atttggttcg gctacaagct gattaaagga actcacttcg tacgctacag cgaaatgaag
    17881 ttcccgcaga acgataagaa ataagtttcc tcccttcctt gctaagccct ctcaaccgag
    17941 agggcttttt caattccatt tccctgacaa atcatgcgga tataaaattt aatatttgga
    18001 ttgataattg ttatcgtttg cattatcgtt acgacacaat caaaaaaggc tgacaaatca
    18061 gaggctgttc cggctttctg ggatgatcac ctgcatacaa ataagtccac cgcgatgctg
    18121 ccgtacgcaa ggggacgtaa agaagatgtg agcgataccc attttatttt cgtagttacc
    18181 tcatggagat atggaatgtt taggttgaac cctttcgtac gggtcgggct gtgtttgtcc
    18241 gctatttctt gtgcatggcc tgtgttagcg gtcgatgatg atggcgaaac gatggttgtc
    18301 actgcatctt ccgtggaaca aaatcttaaa gatgcacccg ccagtatcag cgtcattacc
    18361 caggaagacc tgcagcgaaa accggtacag aatctgaagg atgtcctcaa agaagtgcct
    18421 ggcgtacaac tgacgaacga aggggataac cgtaagggcg ttagtattcg tggtctggac
    18481 agcagctaca ccctgattct tgtcgacggt aaacgcgtta actcccgcaa tgccgtcttc
    18541 cgccacaatg atttcgatct gaactggatc ccggtcgatt ccatcgaacg tattgaagtg
    18601 gttcgtggcc cgatgtcgtc gctatacggc tccgatgcgc tcggcggtgt agtgaatatc
    18661 atcaccaaaa aaatcggtca gaaatggtcg ggtaccgtta ccgtcgatac caccattcag
    18721 gaacatcgcg atcgcggtga tacctataac ggtcagttct ttaccagtgg accattaatt
    18781 gatggtgtgc tgggaatgaa agcttacggc agcctggcaa aacgtgaaaa ggatgacccg
    18841 caaaactcaa cgaccaccga taccggagaa acgccgcgta ttgaaggatt ctccagccgc
    18901 gacggcaatg tcgaatttgc ctggacaccg aatcaaaatc acgattttac tgccggatac
    18961 ggtttcgacc gtcaggatcg tgattccgat tcgctggaca aaaaccgcct ggaacgccag
    19021 aactactgcg tcagccataa tgggcgttgg gattacggca ccagcgaact gaaatcctac
    19081 ggtgagaaag tcgagaacaa aaaccctggc aacagcagcc cgataacttc cgaaagcaat
    19141 acggtcgacg gcaaatacac gttgccgctg acggcgatta atcagtttct cacggttggc
    19201 ggtgaatggc gtcacgacaa acttagcgat gcggtgaacc tgaccggggg aaccagctcc
    19261 aaaacgtctg ccagccagta cgcgctgttt gtggaagatg aatggcggat cttcgagccg
    19321 ctggcgctga cgaccggcgt gcgtatggac gatcacgaaa cctacggtga acactggagt
    19381 ccgcgtgcct acatggttta taacgccacc gacaccgtaa cggtgaaagg gggctgggcg
    19441 acggcattta aagcaccttc tctgttgcaa cttagccctg actggacgag caattcctgc
    19501 cgtggcgcat gtaagattgt gggtagcccg gatctgaaac cagaaaccag cgaaagttgg
    19561 gagctggggc tttactacat gggtgaagaa ggctggctgg aagggggtga atccagcgtt
    19621 atcgttttcc gtaacgatgt gaaagatcgt atcagcatca gccgtacgtc tgacgtcaac
    19681 gctgcaccgg gctaccaaaa ctttgttggt tttgagacgg gcgctaacgg acggcgcata
    19741 ccggtattta gctactacag cgttaacaaa gctcgtattc agggcgtgga aaccgaactg
    19801 aaaattccgt tcaacgatga atggaaactg tcgatcaact acacctacaa cgatggtcgt
    19861 gatgtcagca acggcgaaaa caaaccgcta tccgatctgc cgttccatac tgctaacggt
    19921 acgctggact ggaaaccgct ggcgctggaa gactggtcat tctatgtttc tggtcactat
    19981 accgggcaga aacgcgccga cagcgcgacg gctaaaacac cgggcggtta taccatctgg
    20041 aataccggcg cggcctggca ggtgactaaa gacgtcaaac tgcgcgcagg cgtgctgaac
    20101 cttggcgaca aggatctcag tcgtgacgac tacagctata acgaagacgg acgtcgttac
    20161 tttatggcag tggattatcg cttctgatga gaagatgccc ggcgaaccgg gcggactttc
    20221 acttcagtaa atactgcgca tggaagcgca ggtgatcctc tataaaagag gcgatgaagt
    20281 agtaactgtg atcatatccc ggctgaatac gcagcgtcat cggccacgct ttctggcgcg
    20341 cggcttctgc caataccgca ggttgcaact ggtcggcaag aaactgatca ttatcgccct
    20401 gatcgataag cgtcgggatc gcatcctgcg cgttactggc atacatcagc gcgcaactat
    20461 cccattccag catgcatttt tgtcctcacc taaataggtg ctaaacgctt tgattcccca
    20521 cgggacgctg cacggattca caatgggcgc aaaggccgaa acgctggtgt atttacccgg
    20581 atttttcagc gccataatca gcgcaccgtg accacccatt gagtgaccgc taatggcgca
    20641 gcggtcgctg acattaaatt gcgactgaac cagcgccggt aattcatcgc gcagataatc
    20701 atacatccgg taatgcgtcg cccacggcgg ttgcgtggca ttaagataaa agcctgcgcc
    20761 ctggcctaaa tcgtagccat cgtcgttggc aaccttttcg ccgcgcgggc tggtgtctgg
    20821 catcaccagt acaatcccca gttccgccgc tacccgctgg gcacccgcct tggtggtgaa
    20881 gttctcgtca ttgcaggtta atccggaaag ccagtacagc actggtggcg gggtgtgatc
    20941 acgagggggt gggagaaaga tactgaacgt catcgggcag tttaaggtac tggagtcgtg
    21001 tcgccagcgt tgctgccagc cttcaaaaca gcggtgctct tcgagcattt ccatgcgtgg
    21061 ctccttgttg tgttgtttgc aatttcttca tcataccgtt tattcaatgt gctgtgagta
    21121 actttcactt ccgtatttgc ataacgatgt tttaacatct gctgatgaaa ggcagcggca
    21181 attacaataa ttatcgctgt gaatactgga ttatgtgcgc cgcctcacgc acaataatca
    21241 agctgtaaat cagcttaata actttgcccc cacgcagggc ggaggcgtca cacctgcagg
    21301 agaaatcata aatgccatca ctcagtaaag aagcggccct ggttcatgaa gcgttagttg
    21361 cgagaggact ggaaacaccg ctgcgcccgc ccgtgcatga aatggataac gaaacgcgca
    21421 aaagccttat tgctggtcat atgaccgaaa tcatgcagct gctgaatctc gacctggctg
    21481 atgacagttt gatggaaacg ccgcatcgca tcgctaaaat gtatgtcgat gaaattttct
    21541 ccggtctgga ttacgccaat ttcccgaaaa tcaccctcat tgaaaacaaa atgaaggtcg
    21601 atgaaatggt caccgtgcgc gatatcactc tgaccagtac ctgtgaacac cattttgtta
    21661 ccatcgatgg caaagcgacg gtggcttata tcccgaaaga ttcggtgatc ggtctgtcaa
    21721 aaattaaccg tatcgtgcag ttctttgccc agcgtccgca ggtgcaggaa cgtctgacgc
    21781 agcaaattct tattgcgcta caaacgctgc tgggcaccaa taacgtggct gtctcgatcg
    21841 acgcggtgca ttactgcgtg aaggcgcgtg gcatccgcga tgcaaccagt gccacgacaa
    21901 cgacctctct tggtggattg ttcaaatcca gtcagaatac gcgccacgag tttctgcgcg
    21961 ctgtgcgcca tcacaactga ttaaaaggca ggaaccatgg agcgcaacgt cacgctcgat
    22021 tttgttcgcg gcgtcgccat tctggggatc ctgctattaa acatcagcgc ctttgggcta
    22081 ccaaaggcgg cttatctcaa tcccgcctgg tacgatgcta ttacgccgca ggatgcatgg
    22141 acctgggcat ttctcgatct catcggccag gtgaaattcc tcacgctttt tgcgctgctg
    22201 tttggtgctg gcctgcaaat gttgctgccc cgtggcagac tctggatcca gtcgcggtta
    22261 acgctgttag tcttgttggg ctttattcac ggtttattgt tctgggacgg cgatattctg
    22321 ctggcttacg ggctggtggg gcttggcgtt ttgctgttat tggggctgat ttccgacagc
    22381 cagaccagcc gcgcctggac gccggatgca tcggctattt tgtatgaaaa atactggaag
    22441 cttcacggcg gcgttgaagc gatcagtaat cgtgccgatg gtgttggcaa cagtttactg
    22501 gcactgggcg cacagtatgg ctggcaactg gcagggatga tgctcattgg tgcggcgctg
    22561 atgcgcagcg gctggctgaa agggcagttc agcttacgtc actatcgtcg tactggtttt
    22621 gtgctggtgg cgattggggt gaccattaac cttcctgcca tcgccctgca atggcagctg
    22681 gactgggcgt atcgctggtg tgccttctta cttcaaatgc cgcgggaact gagtgcgccg
    22741 tttcaggcga ttggctatgc gtcgctgttt tatggcttct ggccgcaatt gagccgcttt
    22801 aagctggtgc ttgcgatcgc ctgcgtcgga cggatggcgc tgaccaacta tctattgcaa
    22861 acgctgattt gtaccacgct tttttaccac ctcggtttgt ttatgcattt tgaccgcctg
    22921 gagctgctgg cgtttgttat tccggtatgg ctggcgaata tcctcttctc tgttatctgg
    22981 ctgcgttact tccgccaggg gccggtggaa tggctctggc gtcagttaac tttgcgtgct
    23041 gccggaccgg caatatctaa aacatcaaga taacgatctg gattacattc gttaacaaaa
    23101 cggctgtaac cgtttccatt gctgtgactc gattcacgaa gtcctgtatt cagtgctgac
    23161 aaaatagccg ccagcaagca gtcatttact gcaatctcat aacaggtagt gaatatgatc
    23221 accattcgtg atgtagcgcg tcaggctggc gtctctgtgg caacggtttc ccgggtgctc
    23281 aataacagca cgctggtcag tgccgacacg cgtgaagcag taatgaaagc cgtgagtgag
    23341 ctggattatc ggccaaacgc caatgcccag gcgctggcaa ctcaggttag cgacaccatt
    23401 ggcgtggtgg tgatggacgt ttctgatgcg tttttcggcg cgctggtaaa agcggtggat
    23461 ctggtcgccc agcagcatca gaaatacgtg ctaatcggca atagctatca tgaagcggaa
    23521 aaagagcgtc acgccattga ggtgttaatt cgccagcgtt gtaatgcgtt gattgttcac
    23581 tcaaaagcat tgagtgacga tgaactggcg caatttatgg ataacattcc cggtatggtg
    23641 ttaatcaacc gcgttgtgcc ggggtacgcc catcgttgcg tttgcctgga taatctcagc
    23701 ggtgcccgaa tggcgacgcg catgttgctg aataacggtc atcaacgtat tggttatctt
    23761 tcttccagcc acggcattga agatgacgcc atgcgtaaag caggctggat gagtgcgttg
    23821 aaagagcagg atattattcc gccggaaagc tggattggca ctggtacgcc ggacatgccg
    23881 ggcggtgagg cggcgatggt tgaactgctg gggcgcaatc tacaacttac cgctgtattt
    23941 gcttataacg acaatatggc cgctggcgca ctgacagcat taaaagataa tggcattgcg
    24001 attccgttac atctctcaat catcggtttc gatgatattc ccatcgcccg ttacaccgac
    24061 ccgcaattaa cgaccgtgcg ttatcccatt gcttcaatgg ctaaattagc caccgaactg
    24121 gccttgcagg gggcagcagg caatattgat cctcgtgcca gccactgttt tatgccgacg
    24181 ttagtgcgtc gccattctgt cgcaacgcgc cagaatgcgg cggcgatcac taactcaaca
    24241 aatcaggcga tgtaaccgct ttcaatctgt gagtgatttc acagtatctt aacaatgtga
    24301 tagctatgat tgcaccgttt taatgttgta acccgtatgt aacagtgaat aatcactttc
    24361 accgaggtaa tagcgtcata acaacaatta aagccgtttt ctggagcgtt accgggcatg
    24421 gaagaacgaa ttttaaaaag tgagcttcgg cgttcagtaa cacttcatta actctactgc
    24481 cccgccgagc atttatctca agcactaccc tgcataagaa aaaccggaga taccatgaat
    24541 aagaaggtgt taaccctgtc tgctgtgatg gccagcatgt tattcggtgc cgctgcacac
    24601 gctgctgata ctcgcattgg tgtaacaatc tataagtacg acgataactt tatgtctgta
    24661 gtgcgcaagg ctattgagca agatgcgaaa gccgcgccag atgttcagct gctgatgaat
    24721 gattctcaga atgaccagtc caagcagaac gatcagatcg acgtattgct ggcgaaaggg
    24781 gtgaaggcac tggcaatcaa cctggttgac ccggcagctg cgggtacggt gattgagaaa
    24841 gcgcgtgggc aaaacgtgcc ggtggttttc ttcaacaaag aaccgtctcg taaggcgctg
    24901 gatagctacg acaaagccta ctacgttggc actgactcca aagagtccgg cattattcag
    24961 ggcgatttga ttgctaaaca ctgggcggcg aatcagggtt gggatctgaa caaagacggt
    25021 cagattcagt tcgtactgct gaaaggtgaa ccgggccatc cggatgcaga agcacgtacc
    25081 acttacgtga ttaaagaatt gaacgataaa ggcatcaaaa ctgaacagtt acagttagat
    25141 accgcaatgt gggacaccgc tcaggcgaaa gataagatgg acgcctggct gtctggcccg
    25201 aacgccaaca aaatcgaagt ggttatcgcc aacaacgatg cgatggcaat gggcgcggta
    25261 gaagcactga aagcacacaa caagtccagc attccggtgt ttggcgtcga tgcgctgcca
    25321 gaagcgctgg cgctggtgaa atccggtgca ctggcgggca cagtactgaa cgatgctaac
    25381 aaccaggcga aagcgacctt tgatctggcg aaaaacctgg ccgatggcaa aggtgcggct
    25441 gatggcacca actggaaaat cgacaacaaa gtggtccgcg taccttatgt tggcgtagat
    25501 aaagacaact tggctgaatt cagcaagaaa taagactgat tgggcgcagt cgtttactgc
    25561 gcccttattg tatggccaac aaggtaaaat tatatggtca gctcaacgac tccgtcttcc
    25621 ggggaatact tgttggaaat gagcggtatc aacaagtcct ttcctggtgt taaggcactt
    25681 gataacgtta atttaaaagt ccggccacat tctatccatg cattaatggg ggaaaacggt
    25741 gcaggaaaat cgacattatt aaaatgcctg tttggtattt atcaaaaaga ctccggcacc
    25801 attttattcc agggtaaaga gatcgatttc cattccgcca aagaggcact ggaaaatggt
    25861 atttcgatgg tacaccagga gttaaacctg gtattacaac gttcggtgat ggataacatg
    25921 tggctggggc gatatcccac caaaggcatg tttgtcgatc aggacaaaat gtaccgcgaa
    25981 accaaagcga tttttgatga actggatatt gatatcgatc cgcgtgcgcg cgtcggcaca
    26041 ttatccgttt cgcaaatgca gatgatcgaa atcgccaaag cgttttccta taacgcgaaa
    26101 attgtgatta tggatgaacc gacttcttcg ttaaccgaaa aagaggtcaa tcatctgttc
    26161 actattattc gtaaattaaa agagcgcggc tgcggtattg tttatatctc gcataaaatg
    26221 gaagaaatct tccagttatg tgatgaagtt accgtattgc gcgacggtca gtggatcgcc
    26281 accgaaccgc tggcaggact gacgatggac aagatcatcg ccatgatggt tgggcgttct
    26341 cttaatcagc gttttcctga caaagaaaac aagccgggcg aagtcatcct cgaggtgcgt
    26401 aacctaacgt cactgcgcca gccgtcgatt cgcgatgtct cgtttgatct gcataaaggg
    26461 gagatcctcg gtattgccgg gctggtgggg gcgaaacgta ccgatattgt tgagacgtta
    26521 tttggtattc gcgagaaatc ggctggcacc attacgttgc acggcaaaca gatcaataac
    26581 cataatgcca acgaagccat aaaccacgga tttgcactgg taactgagga gcgccgctca
    26641 acgggaattt atgcctatct ggatattggt tttaactcgt taatttccaa tattcgcaac
    26701 tacaaaaata aagttggttt actggataac tcgcggatga aaagcgatac ccagtgggtg
    26761 attgattcga tgcgggtaaa aacgccgggt catcggacgc aaattggttc gctctccggt
    26821 ggtaatcagc aaaaggtgat tattggtcgc tggctactaa cgcaaccaga aatattaatg
    26881 ctcgatgaac cgacgcgcgg tattgatgtc ggggcgaagt ttgaaattta tcagttaatt
    26941 gccgaactgg cgaagaaagg caaggggatt attattatct cctctgaaat gcctgagttg
    27001 ttagggataa cagaccgtat tctggtcatg agcaatggtc tcgtttccgg aattgtcgat
    27061 acaaaaacaa caacgcaaaa cgaaattctg cgtcttgcgt ctttgcacct ttaagattag
    27121 gggcttccca tgagtgcgtt aaataagaaa agttttctta cttacctgaa agagggcggt
    27181 atttacgtcg ttcttttagt tttgctggcg attattattt tccaggaccc aacattttta
    27241 agtctgttga acttaagtaa tattctcacc cagtcatcgg tgcgtattat tatcgcgctc
    27301 ggtgtggcag ggttaattgt cacccagggg accgatcttt ctgctggtcg tcaggtaggg
    27361 ctggcggcag tggtggctgc gacattattg cagtccatgg ataacgccaa caaagtgttc
    27421 ccggaaatgg cgacgatgcc gattgcgctg gttattctga ttgtctgtgc cattggtgcg
    27481 gtgatcggtt tgatcaacgg tctgattatc gcttatctca acgtgacgcc gttcattacc
    27541 acgctcggca cgatgatcat cgtctatggc atcaactcgc tctattacga ctttgtcggg
    27601 gcgtcgccaa tttctggttt tgacagtggc ttctctacct ttgctcaggg ctttgtcgcg
    27661 ctggggagtt tccgtctctc ttacatcacc ttctacgcgt tgattgcggt ggcgttcgtc
    27721 tgggtgttgt ggaacaaaac ccgcttcggt aagaacattt ttgccattgg cggtaacccg
    27781 gaagcggcaa aaatatctgg tgtcaacgtc ggcctgaacc tgctgatgat ctacgcgttg
    27841 tctggcgtgt tctatgcctt tggcgggatg ttagaagccg gacgtatcgg ctctgccacc
    27901 aacaacctcg gctttatgta tgagctggat gctatcgcgg cgtgcgtggt aggcggtgta
    27961 tcgttcagcg gcggtgtggg gacggtgatt ggcgtggtga ccggggtaat tatttttacc
    28021 gtcatcaact atggcctgac gtatatcggc gtaaacccat actggcagta catcatcaaa
    28081 ggggcgatta ttatcttcgc cgtagcgctg gattcactga aatacgcgcg taagaagtga
    28141 tgtgattgcg gatattgccg gatgcgtctc tggttttaca acgttaccgg gtgttctttc
    28201 tcgcctttct taaacttcac ttccccgagc tcaatacaac ccaccgggca gacgtgacca
    28261 caaagcagac aacccacaca tttctcggta ttacaatgcg gtgtgcgggt tttctcgctc
    28321 cattccatcg cctggtgacc gccgtcgtaa caggaaatat aacagcgtcc acagccaaca
    28381 catttgtcaa gattgatacg gggatagaca atataactgc ggtctaaatc ttccgccggg
    28441 acaatattgt tattcgccag acctaccatc tcctgcaacg aatcaaatcc ctgatcggcg
    28501 agataatgcg ataacccgct cgccatatct tctacgatgc gatagccgta ctgcataatg
    28561 ccggtggtga cctgtaacgt tgctgcgccg agcaataaaa actccgccgc atcttcccag
    28621 gtttcaatgc cgccgatacc gctgattggg aaatcgcgca gttctggatg ggtgcgcatt
    28681 tgctgaatga agcgcagggc gatcggtttt accgctttac cggaatatcc ggaaatactc
    28741 gatttaccgt taacgatcgg cataccgatt ttctgattaa gatcgatatt ggtgatggat
    28801 ttaacggtgt taattgccgc aatgccatct gcgccgccgc gcttcgccgc cagcgccact
    28861 tcgcacatat caccgatatt cggcgtcatc ttcgccagca ttggcagcgt ggaaccccgt
    28921 ttcactgccc gacaatattt ttctaccagc tccgggcttt gcccgacatc gctacccatc
    28981 gcatgagaag tcatttgcgg acaggagaag ttacactcga tcatatccgc gccagctgct
    29041 cccattgctg ctcattttcc cccatgatcg aagcgatcaa tactttgtcc gggtaatctt
    29101 ccttcagccg acgcagggcg gtcagattct cttccaacgg atgttcagca atctgctcca
    29161 tatttttgaa gccgataaaa ccggtatctt ctttcaccag atgatcaaaa cgcggcgaga
    29221 cttcgttggc gataaaaaag ccgatcgttt taaacaccac accgccccaa cctgtgtcgt
    29281 aggctttggc acacatctca tagcagttgc ctaccggcga agaagagagg cagaacgggt
    29341 tgggaaagtt cacgccgcaa aaagtaatcg aaagatcttt cgttaacatg agcaagctcc
    29401 ctctaaatag tgatgaatcg cctcggcggc ttctttcccg gttttcacgg catagaccac
    29461 ggttttgtca ccctcaacaa tatcgccagc agcaaagact tgcggatcgc gggtctgata
    29521 attttgtgtt ttgatggtgt tacgctgctg ctctaactcc gcaaaggcat ccagtctggc
    29581 atgctgaccg acggcgagaa taattttatc tgccgccatc gtcagttcgc ctgataaccg
    29641 tacatgctta aacgtgactt tattgccttc cacggctact ggcgtgaatc catcgatgat
    29701 cgaaacgccc agctcccggg cgctggcaaa ctctttttcg cttgccggaa actcatctaa
    29761 ctcttcacgt gctacgcaag ttaccgcctg acagccgaga actttcagcg tgctggctac
    29821 gtccatcgcg acatcaccgc cgccgataat taatgcgctt tgtggaatgc tgatatcgcc
    29881 ttgcgcctgt cgtacacgtt gcaggaagtc gacggcaatc tcaacgtcgc tatgctcaaa
    29941 cagcggtagc ccggaaccgc ttgataaccc cacggtgacc agtaccgcgc ggttttccgc
    30001 tttcagttgc tccagggtga gtgttttacc gatttcgtta ttgcacttaa tggtcacgcc
    30061 cattttttca atacgggcga tctctgcatc cagcactgat tgcggtaacc ggaattgcgg
    30121 aataccgtta cgcaaccagc caccggggtg cgcttctttc tcataaatcg tgacgtcata
    30181 accctggttt gtcagtgtca cactggcctg caatccggca ggacctgcgc caataatcgc
    30241 gactttgccg agcgttttag taccgggctg ataaatttcc atcccggttt gttgttcaaa
    30301 atcagtaaca aaacgctgta agcggccgat atcaatggga gcatcaacac cggcacgggt
    30361 acaaccgctt tggcataatt tctccgtcgg gcacactctg gcacaaacgg caccgagggc
    30421 attattttcg cgaattgtct cggcagcgcc tttaaaatta cgaaagtaga ttgagcgaat
    30481 aaatttcccc ggatcggtct gtgccgggca agcctgacta cagggagcgt cgtgacataa
    30541 taaacagcga gaggcttctt taatcgccag taacggcgta aaagccggag tgagttcatc
    30601 cagataattt tgttgcggca tgtgatcttc cttaatgcta tgagtaatta tggcgaacgc
    30661 taaataaata ttgcgtgctg atattaaggc tatgcgcgtg gggaaattta agctgtgaaa
    30721 ggattcacaa atagctgcgg ttcagaatgt tatatccgca gcttattgag tgaagtaata
    30781 attattctca ataattaaca gaaaagagaa aatattaacg atagaaaata agataaacaa
    30841 tcatgccgat agataaaatt ccgaagccaa tatatgccac catataagcg aagaagagtc
    30901 cgggattttt tgctcgccag acatacaccg gttccggtgc atttttaacc cgtttatcaa
    30961 tcgcgcctgc gcgccagcct ttccatgctt cgcggaaaca aaatatcggg ataaggaaaa
    31021 aaacggcaac gacaaaaaac tgctgtacat ccataactac tttccttgtt tcttttgtaa
    31081 ttcaagtaac tgttcgggtg taacggcggg atacccctgc gcatcttccg gcacctggtg
    31141 catagccgga atagggacca gcggccctaa aaaacgcggt tcacgtttaa aaatataaag
    31201 gtcagccagc gcaccgaaac gggcggcaaa ttcgcgaata cgtactgaca gcatatcttt
    31261 cggtgacggt acggcataac actgagcctg aatccccata tgcagcgcaa taaataatgc
    31321 tcgctcacag tggaaacgtt gggtgataat aatgaaatca ttagtatcga aaactttgcg
    31381 tgtacgcacg atggaatcca gcgtacgaaa gcctgcgtaa tcgagaacaa tatctgatgg
    31441 gtcgacacca gcagcgatta aatctttgcg catggtcatc ggctcgttat aactttgcaa
    31501 tgcgttatcg ccgctcagta atagataatt taccttaccg ctgttatagg cattaatcgc
    31561 tccttgaatg cggtagcgat aatactgatt aataacgccg gtacgataat attttgctgt
    31621 gccgagcacc acgccgacct ggcggtaggg gagatcctgc aattcgtcgt agatataagg
    31681 cgcggttttc cagctcatcc agcgatcgag gccgagcaca gtcaacagca gcaagccgat
    31741 caggactaac aggctgagga acacgcgctt taacatgcag gagactcgag ctaaggaaaa
    31801 tttccatcag gctactttac ccgctggttt tgcgcaagaa acggcaaact gtttgccggg
    31861 aatttcgaca ccaggagagg gctggcaagc cggacaaggc gttcacgccg catccggcac
    31921 caggcttaag cgagaagcac gcggtcgata ctgtgacagc cgagagcttt cagcgttgcg
    31981 gaggtggcat cccactgaat caacggcgca tcggcttttt ctgccagaac cgcgcgctgg
    32041 atatccgggt aatcataacc cttgagattc aacagaatta acgccccctg caacggcggc
    32101 agagtcgggt tgaatgcggc gttttcagcg tagctgccgc tgaaaatacg accgtcttta
    32161 cattccagcg cgacaccgct tggtgactta ctgtaaggca tgtgcgaacg gtttgccgct
    32221 gcaatcgccg cctgagaaag cgcatcacca gtcagcgcat agccgtgatc ctgctcgtcc
    32281 atcagcagcg ttttaatctc cagatctttc ggcccaaagg catccggcag atagtcacgc
    32341 agcgcgtgtg cctcgcggcc cggcaaatga atacgcagat ccagaccgct gttcagttca
    32401 ttcataaact gacggcagtg accacaaggc gtatagttaa cggtgatggc tgcaagcgct
    32461 ttttcaccac tcaaccaagc gtggctgatt gcgctttgtt cggcatgaac ggtttgctgc
    32521 attgttgcac caataaattc catattggca ccaaaatacc aggttccgct cacaccgcgc
    32581 gcaattgcgc caacattaaa attcgacaat ggcgtacgcg cacaggccgc cgccagcgga
    32641 agtagtgcga atgccagcgc gtcttcgtcc agtcccgttg cgctcttcag cgatgagact
    32701 tgctccccgg tcaacaaagc ggggaagtac ttgtctgcca gaataggttc cagtgcagat
    32761 tgcaagttat ccgcaagttg ggcaaaagcg gtttgaaaac gtggatgcat ggcgttgcct
    32821 cataatcatg taatgggata acagtttacg aacccgttat ggctttatat gtgatctgaa
    32881 tctcattaat tatgcaacac gtgaaactta catcaaaaat gcgcgacgta tcgcaaattt
    32941 tagcccatta ctgccagaat aatcgggaaa aggaacggtg cgatcagcga agtaattatc
    33001 ccgcataaca ccagcgctag cgaactaaat gcaccttcct gataatccag ctcggcgcag
    33061 cgcgccgtac cgagggcgtg cgaggcagtc cccattgcca gaccgcgcgc agctttggta
    33121 cgaatacgca tcgcattaag caatgtatgg ccaaataccg cgccgaggat gccgacgaaa
    33181 atcacgcaaa ctgcgctgat tgccggaata ccgccaatac tgccgccaac cgccattgca
    33241 ataggcgtgg tgactgattt cggcaggatt gacgcggcga tttccggtga agcgcccatc
    33301 aacaatgcca cggaagtccc cgtcaccatc gcaaccacgc tgccgataaa acagatggtg
    33361 atgatcgatt tccagcgcgc gcggatctgg tgtagctgct catataaagg ataggccagc
    33421 gcgacgaccg ccggttgcag cagatcgttt agcacttcgc tacctttaaa gtaactgtcg
    33481 taagagatgc cggtcagcat taaaaaagga atgatcacca ccattgctac cagcaacggg
    33541 ttaagcaggg gaaacttata ccgtgccgcc agtttgcggg cggcaaaaaa gacaatcaac
    33601 gtcagcggta atgaccacca gatattcgcc atcattcttc tgatcctttc tgacctacga
    33661 ctttacgttc accgtgtacc agttgtgaac tccagctcac caccagaaaa accaccagcg
    33721 tactgacagc acaggaaacc actaccgggc caaactgcgc gcggagcaaa tcaaaatatt
    33781 gcatgacgcc tacgccaatc ggcacaaaca atagcgccat atagcgaatc agtacgtagc
    33841 accccggatt gacccatttc gccggaagaa tttgcaaggc cagcaggaca aacaggatct
    33901 gcatcccgat gatgctgccc ggaatggtta ccggtagcag ggaagcaatg aaaatgcctg
    33961 catacaggca ggcataaatc aggatgaaag cgcgtaaata ttgccagata atgttcagtg
    34021 tcttactcat aatcaacacc ccagatgaga tggattcatc atacaattaa ttcgtaaaac
    34081 gtgctaccga tcacattaag aaatcagcgc ttattttttc gctacccggt attacgcaat
    34141 gagcagcgaa ctcaggcata catcaagcat agagagggat tgcagtttgc agcaatccct
    34201 ctttgcagac ctttaatccg ctgaccatag gcgctaacat agcgcctcat ttttttgcgg
    34261 gtgatgatat gcgtgtgtta ctggcaccga tggagggagt gcttgactct caggtgcgtg
    34321 aattgctgac cgaagttaac gactacgatc tgtgcatcac cgagtttgtc cgcgtggtgg
    34381 atcaactgct gccggtaaaa gtctttcatc gcatttgccc tgagctacaa aacgccagcc
    34441 ggacaccatc tggtacgctg gtgcgcgtgc agttgttagg tcagttccca caatggctgg
    34501 cagagaacgc cgcccgtgcg gtggagttag gtttctgggg cgtggatctc aattgcggct
    34561 gcccgtcgaa aacggttaac ggtagcggcg gcggggcgac gttactcaaa gatcctgaac
    34621 tcatctacca gggggcaaaa gcgatgcgtg aagctgtacc ggcgcatttg cctgtcagcg
    34681 tgaaagtgcg tctgggctgg gacagcggtg agaagaaatt tgaaatcgcc gatgcggttc
    34741 aacaggctgg cgctacggag ctggtggtgc atgggcggac gaaagagcag ggttaccgcg
    34801 cggagcatat tgactggcag gcgattggcg agattcgcca gcggctgaat attccggtga
    34861 ttgccaacgg tgaaatctgg gactggcaga gcgcgcaaca atgcatggcg atcagcgact
    34921 gcgacgcagt gatgattggt cgcggggcgt tcaatattcc caacctgagc cgggtggtaa
    34981 aatataacga accgcgaatg ccgtggccgg aggtggttgc tttgctgcaa aaatataccc
    35041 gtctggaaaa gcagggcgat accgggttat atcacgttgc gcggattaaa cagtggttga
    35101 gttatttgcg taaagaatac gatgaagcaa cggaattatt tcagcatgtt cgggtgttga
    35161 ataactcccc tgatattgca agggctattc aggcaattga tatcgagaaa ctctaaaaac
    35221 catagtgatg tttttgacaa tgtgttagct ctttatcaat aggccgcacg gttgtttggt
    35281 ggcgtcaaaa tcgccgaggc gttccctgaa ggccggggca gcccacatgg atgtgggctg
    35341 agggcgcgtt ttacagggat gttacctcgc gcccgacccg gtagccgtaa gggataagcc
    35401 gagggcaccg cgcagcggcg attttgttcg ccagagcccg ggggggcagg gggcggcggc
    35461 gattggtcgc cccctgcgcg ctccttgcgc cagtgattat atgaagcaat gcacatgaca
    35521 ggagcgcaac aggatgagag actgaaatat tctcgttatt ttcggatctg atgacattaa
    35581 ttaattttaa aatatcatct taaatacgcc agtcaccacc agtagcccaa tcaggaaaat
    35641 aatcaataca gcccagagta taattttcat tggcgttcct ttatttgtga gtgctgaata
    35701 agtataggaa aaaacgaagt cctttgccgt tatccataaa cgattaagat aaatcggaag
    35761 atatcattta cttagtctat atttttctgg gtatgtcgtt ttattgctac tgatgtattg
    35821 ccacgcgaaa ctaaaaaata tatcgcaaca cacggtaatc tccgcgcacc gtttcttacc
    35881 tgattattcc cccatgaatc gtgattcctt ttatccagcc atcgcctgtt ttccgctgtt
    35941 actgatgctg gccgggtgtg cgcctatgca tgaaacccgc caggcgttaa gccagcaaac
    36001 gcccgctgca caagttgaca ccgcattacc cacggcgctg aaaaatggtt ggccagacag
    36061 ccaatggtgg ctggagtatc acgataatca gctcacttca ttaattaaca atgcactaca
    36121 gaacgcaccg gatatgcagg tggctgaaca gcgcattcag ctagctgaag cccaggcgaa
    36181 ggcagttgcc actcaggatg gtccgcagat cgacttttcg gcggatatgg agcggcaaaa
    36241 aatgtcggca gaagggctaa tggggccgtt tgctctgaac gatccggccg caggtacgac
    36301 cggtccgtgg tacaccaacg gaacttttgg cttaacggcg ggctggcatc tcgatatctg
    36361 gggaaagaat cgggcggagg ttactgcccg cctgggtacg gttaaagcac gggcggcgga
    36421 acgcgagcaa acccgccaat tgctggctgg cagcgtagcc cgcctgtact gggagtggca
    36481 aacccaggcg gcgttaaaca cggtcttgca gcaaatagaa aaagagcaga acaccattat
    36541 cgcgaccgat cgccagctat atcagaacgg gattacttct tcagttgacg gtgtggaaac
    36601 cgatattaat gccagcaaaa cccggcagca gctcaacgat gtcgcgggga aaatgaaaat
    36661 tattgaggca cggttaaatg cactcacaaa tcaccagact aagtcattga agcttaaacc
    36721 ggtcgcgttg ccgaaagtgg caagccagct tcctgatgaa ctggggtact ccttactggc
    36781 ccggcgggca gatttgcagg cggcgcactg gtacgttgag tcatcgttaa gcactattga
    36841 tgcggcaaaa gcggcatttt atcctgacat caacctgatg gccttcctgc aacaggatgc
    36901 gttgcactta agcgatctgt tccgtcattc cgcgcagcaa atgggcgtta cggcaggcct
    36961 gacgctacct attttcgata gtggtcgtct taacgccaat ctcgatatcg caaaagccga
    37021 aagcaacttg tctatcgcca gctacaacaa agcggtggtt gaagcagtga atgacgtggc
    37081 gcgggcagcc agtcaggttc agacactggc ggagaaaaac cagcatcagg cgcaaattga
    37141 gcgcgatgcc ttgcgtgtgg taggtcttgc gcaggcccgc tttaacgcgg gcatcattgc
    37201 tggttcccgc gtcagcgaag ccagaatccc ggcgctgcgt gagcgggcca atggcctgtt
    37261 attgcaaggg cagtggctgg atgcctccat tcgactcacc ggtgcgctgg gcgggggtta
    37321 caaacgctga tgatatgcgc cttctatact taacgtttat tcagcgttaa gtggagaact
    37381 cgatggcata ggtcgcgata attaccgctt ccgattcggg gatcggcaaa gagtgcgcgt
    37441 tattactggc gcagcagggg tttgatattg gcattacctg gcactcagat gaagaagggg
    37501 caaaagatac cgcgcgtgag gtggttaccc acggcgtacg tgcggagatc gtgcaactgg
    37561 atctcggcaa tctgccagaa ggtgcgcagg cgctggagac actcatccag cgatttgggc
    37621 gtattgatgt gctggtgaat aatgcgggtg caatgaccaa agcgccgttt cttgatatgg
    37681 cttttgatga gtggcgcaag atttttaccg ttgatgttga tggtgcattt ttatgctcgc
    37741 aaattgcggc tcgtcagatg gtgaaataag ggcagggcgg tcgcatcatc aacattacgt
    37801 cggtacatga acatacgccg ctgccggatg ccagcgccta cacagccgct aaacatgcgc
    37861 tcggcgggtt aaccaaaacg atggcgctgg agttggtcag gcataagatt ttggtgaacg
    37921 cagtcgcgcc tggggcgata gccacgccaa tgaacagcat ggatgatagc gacgtgaagc
    37981 ccgacgcgga gccttcgatt cctctacggc gttttggcgc aacgcatgag attgccagcc
    38041 tggtggtgtg gctttgttcg gagggcgcaa attacacgac agggcagtcg ttgatagtgg
    38101 atggcggctt tatgttggcg aatccgcagt tcaacccaga atagcgcagt gaggtgcgtg
    38161 gatatttcaa tgggttaggc cagataagac accagcgtcg catcaggcat ggaacacccg
    38221 gtgccggatg tggagtgaat gcttatctgt ggcaactgct ttacgcctga ttatccggct
    38281 ttttcttccc gcgccgtttc agccaccagc gcacgcccac caccagaacc acgaccagaa
    38341 tcaaccagac ccagtgcttc aaatgctggt cgagattgtg caaccacggc gcaatcacct
    38401 gaccaccagc gtaaccaata gtggtaaaaa tcaacgccca ggcaaatgcg ccgagaatat
    38461 tcagcggcag aaagattttc ggcggcagct ggctggcacc aatcagcgtc gggccaatca
    38521 cccgaaagcc atacataaag cgcgtaccaa tgacaaacag atacggatgg cgttggataa
    38581 gtttctgtgc ccgctcaatt ttatcctgat gtttcgagaa acggcgtaac agcttgccgc
    38641 caaaccgccg cccgcacaga tagagcacct ggtcgccaat catgccacca agcgccacag
    38701 aaagtaccac cagtgggaac tttaatagcc cctgatgtgc cgcaacgcct cccagcaaag
    38761 tcacggtttc accttccgcc aggctaccga taaccagcgc ggcataacca tattgtgaga
    38821 tgagtgtatt gagagccata ggccagtttg tctccctttg gttgcctgta agcatacacc
    38881 ctgtgaatca ttcacgctga aaaaggacat acatcagacg gtgcaaatta catggatgaa
    38941 caaaaaataa tcacaagctg gcttatactt gaactgtcag gttcatgaac tcacaacaag
    39001 ggggcggtat gagccatgtc tggggactgt tttcccatcc cgatcgtgaa atgcaggtga
    39061 ttaatcgcga aaacgaaacg atttctcacc attataccca ccacgttttg ctgatggcgg
    39121 cgattccggt gatttgcgcc ttcattggca ctacacagat tggctggaat tttggcgatg
    39181 gcactatcct gaagttatcc tggtttaccg gactggcgct ggctgtcttg ttttacggcg
    39241 ttatgctggc tggggttgcg gtgatggggc gggtcatctg gtggatggcg cgcaattacc
    39301 cgcagcgtcc gtctcttgcg cactgcatgg tcttcgcggg ctacgttgca actccgctgt
    39361 ttttaagcgg tctggtggcg ctttatccac tggtctggtt gtgtgcgctg gtcggcacgg
    39421 tggcgctgtt ttacaccggc tacctgcttt accttggtat tccgagcttc ctgaatatca
    39481 acaaagagga aggccttagc ttctccagtt cgacgctggc gattggcgtg ctggtactgg
    39541 aagtgctgct caccctaacc gttatactat ggggctacgg ttatcgtttg ttctagaaag
    39601 ttctttgacg ttgcattgct ggcgtaaatg tcagcaatgc agcatttact cacgattctc
    39661 ctttgacgat cgcacttttt gctcgttatg atggcgtcgc tagcctcagt aaatccttac
    39721 tccggcggtg cgcaacccgt gcgcgtgaac cactatctga atgctcatca tgccgaaatt
    39781 tcgagtttct ttatttagct tggccctgat gctggctgtg ccttttgcac cgcaggccgt
    39841 tgcgaaaacg gcagccgcta ccaccgcttc acaaccggaa attgcctccg gtagcgcgat
    39901 gattgttgat ctgaatacca acaaagtgat ctattcgaac cacccggatc tggtgcgtcc
    39961 gattgcgtct atcagcaaat taatgaccgc gatggttgtg ctggatgcac gactgccgct
    40021 ggatgaaaaa ctaaaagtgg atatcagcca gacgccggag atgaaagggg tctattcgcg
    40081 cgtacgactg aatagcgaaa tcagccgtaa agatatgctg ttgctggcgc tgatgtcttc
    40141 agaaaaccgc gcggcggcaa gccttgcgca ccattatccc ggtggttaca aagcctttat
    40201 taaggcaatg aatgcgaaag cgaaatcgct cggaatgaac aacacgcgct ttgttgaacc
    40261 taccggattg tcggtgcata acgtttcaac tgcccgtgac ttaaccaaac tgctcattgc
    40321 cagcaaacaa tatccgttga tcgggcagtt aagtactacc cgggaagata tggcaacctt
    40381 ctctaacccg acgtatacgc tgccgttccg caatactaat catctggtgt atcgcgataa
    40441 ctggaatatt cagttaacca aaaccggctt taccaatgcg gcgggccatt gtctggtgat
    40501 gcgtacggtt atcaataata aaccggtggc gctggtagtg atggacgcgt ttggcaaata
    40561 tacccatttt gccgatgcca gccgcctgcg tacctggatt gaaaccggta aagtgatgcc
    40621 tgtgccggca gcagcgttga gctataaaaa acaaaaagcc gcccaaatgg cggcggcggg
    40681 gcagacggca cagaacgatt aagtgaattc ggatggcgat actctgccat ccgtaatttt
    40741 tactccactt cctgccagtt tttccgttta ctggttttac cgatccccgg attcatgcta
    40801 ttggtcggat cattctcgcg atagaacttc tgcaacgtct ccggtgcttt atacaaatga
    40861 ccgacgttat gctcggcagg gtactgcgcg ccgcgctgct gtagcagttc cagcatctgt
    40921 tcttttaacg catgcacatc cacgcctttt ttcactatgt aatcctgatg gaagacatag
    40981 cacataaaat ggccgtaata gagcttgtgc accagctggc tgtcgatctc cggcggtaaa
    41041 tgctcatacc actcggtgtc gttacgccgc agagcgatat ccagtgccag aatgtcttcg
    41101 acttcatcgg aatgcaccgc ctgataacga attgctgcgc ccgcagcggc gaaacggtgt
    41161 aaaaacgctt tgctaccttc ctccggcgta cagacaaaga aatcgccctc ggcctgtttg
    41221 aaataatcca ccagccacga tttggcttca ccgacgccat cgcctgccat ttttaacagc
    41281 agatgatgct cgtatttatc gcgccagttt ttcatgcgcg gcggtaaatg gctggggaac
    41341 aggtgaccga atttttgcat tgcacggtca gtaaaatgcg gacggaagaa tttcactttc
    41401 tccagcatcg catcggtgcg tcccttgaga ttaaagaaga acggcatctt gtcggtgcct
    41461 aacttatcaa tcatcaggaa ggtgtctttg ccgtattttt ccgcaatatc gtagatatcc
    41521 cggtgcatat attccccggc aaccggcaga ttttcgaagt tagccagaat atgacggcgg
    41581 atttcggtca gcacttccgg ctggttggtg ccgatataaa acacctgctg atttttttcc
    41641 gcttcgaagg tatcaagacg taccgcaaag acggccagct tcccggcgca accagaagat
    41701 tcaaataacc gatccggatc ggcgttataa cgtgcgggcg tgtcggcttc aatatcacga
    41761 acgcggtgga catagtcata atcgtgggcg tgacgaccat cgtgacgcac atcgtcatct
    41821 ttgatgcgat cgtcatccag cttgctaagg atctgctccg gcgtttcgcc cagatcaatc
    41881 cccagatggt tcaccagcgt cagtttgcca tcttcatcta tacgcgcgaa taacgacatt
    41941 tcggtatacg ccgggccacg ttgcaccagc gagccgccgg agttgttaca aataccgccg
    42001 atgaccgatg cgcctataca cgatgatcca atcaccgaat gcggttcgcg tcccagcggt
    42061 ttgagggctt tttccagcga atagagcgtg gtgcccggat aggccagcac ctgttcgccc
    42121 ttgccgagaa cgtgcagctt gtcgagacgc agggtgctga tgatcacgat atcgcgatca
    42181 tagtcgttac cgtttggcgt cgatccttcg gtcaggcctg tattggcagc ctgcatcaga
    42241 ataattttgt cggcggtgac gcaggctttc agcacccgcc acaattccag tagtgagcca
    42301 gggaaaacga cagccagcgc gtcgccctga ccagaacgga agcccttgcg atagcgggcc
    42361 gtttttgcgg gatcggtgag caggtgtgaa tgaccgacca gacgggcaag ttcattcaaa
    42421 aaggctttat tatcagttgt tgtcatggaa gacattttcc actccttgtg gtggcgaaaa
    42481 aatatcacaa actagaatag cgccttgtat gacaattcag cgagtgaata ttttgaaaat
    42541 tcagacaata aactgagcat tctttttctc tctccatcat gcttatggca aactacggtt
    42601 tttgcaccat tttcgctgcc acgtcgggca acaaaggaag aaaaatccat atgaaatggc
    42661 tatgttcagt aggaatcgcg gtgagtctgg tcctgcagcc agcactggcg gatgatttat
    42721 tcggtaacca tccattaacg cctgaagcgc gggatgcgtt cgtcaccgat ttgctcaaga
    42781 aaatgacggt tgatgagaaa attggtcagc tgcgcttaat cagcgtaggc ccggataatc
    42841 cgaaagaggc gatccgcgag atgatcaaag acggtcaggt tggggcgatt ttcaacaccg
    42901 taacccgtca ggatatccgc gccatgcagg atcaggtgat ggaattaagc cgcctgaaaa
    42961 ttcctctttt ctttgcttac gacgtgctgc acggtcagcg cacggtgttc ccgattagcc
    43021 tcggtctggc ctcgtctttt aacctcgatg cggtgaaaac ggtcgggcgt gtctctgctt
    43081 atgaagcggc agatgatggc ctgaatatga cctgggcacc gatggtcgat gtctcgcgcg
    43141 atccgcgctg gggacgtgct tccgaaggtt ttggcgaaga tacgtatctc acctcaataa
    43201 tgggtaaaac catggtggaa gcgatgcagg gtaaaagccc ggcagatcgt tactcggtga
    43261 tgaccagcgt caaacacttt gccgcatacg gcgcggtaga aggcggtaaa gagtacaaca
    43321 ccgtcgatat gagtccgcag cgcctgttta atgattatat gccgccgtac aaagcggggc
    43381 tggacgcagg cagcggcgcg gtgatggtgg cgctgaactc gctgaacggc acgccagcca
    43441 cctccgattc ctggctgctg aaagatgttc tgcgcgacca gtggggcttt aaaggcatca
    43501 ccgtttccga tcacggtgca atcaaagagc tgattaaaca tggcacggcg gcagacccgg
    43561 aagatgcggt gcgcgtggcg ctgaaatccg gaatcaacat gagtatgagc gacgagtact
    43621 actcgaagta tctgcctggg ttgattaaat ccggcaaagt gaccatggca gagctggacg
    43681 atgctgcccg ccatgtactg aacgttaaat atgatatggg gttgtttaac gacccataca
    43741 gccatttggg gccgaaagag tctgacccgg tggataccaa tgccgaaagc cgcctgcacc
    43801 gtaaagaagc gcgtgaagtg gcgcgcgaaa gcctggtgtt gctgaaaaac cgtctcgaaa
    43861 cgttaccgct gaaaaaatcg gccaccattg cggtggttgg gccactggcg gacagtaaac
    43921 gtgacgtgat gggcagctgg tccgcagccg gtgttgccga tcaatccgtg accgtactga
    43981 ccgggattaa aaattccgtc ggtgaaaacg gtaaagtgct gtatgccaaa ggggcgaacg
    44041 ttaccagtga caaaggcatt atcgatttcc tgaatcagta tgaagaagcg gtcaaagtcg
    44101 atccgcgttc gccgcaagag atgattgatg aagcggtgca gacggcgaaa caatctgatg
    44161 tggtggtggc tgtagtcggt gaagcacagg ggatggcgca cgaagcctcc agccggaccg
    44221 atatcactat tccgcaaggc caacgtgact tgattgcggc gctgaaagcc accggtaaac
    44281 cgctggtgct ggtgctgatg aacgggcgtc cgctggcgct ggtgaaagaa gatcagcagg
    44341 ctgatgcgat tctggaaacc tggtttgcgg ggactgaagg cggtaatgca attgccgatg
    44401 tattgtttgg cgattacaac ccgtccggca agctgccaat ttccttcccg cgttctgtcg
    44461 ggcagatccc ggtgtactac agccatctga acactggtcg tccgtataat gccgacaagc
    44521 cgaacaaata cacttcgcgt tattttgatg aagctaacgg agcgctttat ccgttcggct
    44581 atggtctgag ctataccact ttcaccgtct ctgatgtgaa actttctgcg ccgaccatga
    44641 agcgtgacgg caaagtgacg gccagcgtgc aggtgacgaa caccggtaag cgcgaggggg
    44701 ccacggtagt gcagatgtac ttgcaggatg tgacggcttc catgagtcgc cctgtgaaac
    44761 agctgaaagg ctttgagaaa atcaccctga aaccgggcga aactcagact gtcagcttcc
    44821 cgatcgatat tgaggcgctg aagttctgga atcaacagat gaaatatgac gccgagcctg
    44881 gcaagttcaa tgtatttatc ggcactgatt ccgcacgcgt taagaaaggc gagtttgagt
    44941 tgctgtaatg ctttagtaag ggcgcgacgt ttgcgccctt tgcctgatgt tcatatctta
    45001 acttgctgaa ttttcgccct ttgtaggccg gataaggcgc tcgcgccgca tccggcagga
    45061 aacgccggac cgaatatgcc tcttaagtat ttatctgatt ctgcaactga atccttccgc
    45121 tcaggctaac tctgtcatta tcaactatgc ttttctctta attcgctgaa aacaacagta
    45181 aaagaatgag gaaagcatca tgccactctt aaagctctgg gcaggttcac tggttatgtt
    45241 ggcagccgtg agcctgccgc tacaggcggc ttcccccgtt aaagtcggtt caaaaatcga
    45301 taccgaaggc gcgctgctcg gcaacatcat ctttcaggta ctcgaaagcc acggcgtacc
    45361 gacggtgaat aaagtgcaac tcggaacgac tcctgtgggg cgcggggcga ttacttccgg
    45421 tgaactggat atctatccgg aatataccgg caatggcgct ttcttcttta aagatgaaaa
    45481 cgatgcagcg tggaaaaatg ccgggcaggg ctacgagaaa gtcaaaaagc tcgatgcgga
    45541 gcacaacaag ttaatctggc tgacgcccgc gcctgcaaat aacacctgga ctatcgccgt
    45601 gcgtcaggat gtggcagaga aaaacaaact cacttcgctt gccgacctga gtcgttatct
    45661 gcaagagggc ggcaccttca aactggcagc ctcggcagag tttatcgaac gcgccgatgc
    45721 gttacccacg tttgaaaaag cctatggctt taagctcggt caggatcagt tgctgtcact
    45781 ggctggtggc gacacggcgg tgacgattaa agccgctgcc cagcaaacct ctggcgttaa
    45841 tgccgcaatg gcttacggca ctgacggtcc ggtcgcggcg ctggggctgc aaaccttaag
    45901 cgatccgcaa ggcgtgcaac ctatctacgc gcctgcacca gtggtgcgtg aatcggtgtt
    45961 gaaggagtat ccgcaaatgg cacagtggct acagccagtc ttcgccagcc tcgatgcaaa
    46021 aacattgcag caactgaatg ccagcattgc agtggaagga ctggatgcca aaaaagtggc
    46081 tgccgactac ctgaaacaaa aagggtgggc gaagtaattt cccgtgactt atctacgtat
    46141 taatcctgtt ctggcgctgc tgttgttgct gacggcaatc gcagcggcgc tgccgtttat
    46201 cagttacgcg cctaatcgtt tagtctcggg tgaagggctt catctctggc aactgtggcc
    46261 gcaaacgatc tggatgctgg tgggcgttgg ttgcgcctgg ctgacagcct gttttgtccc
    46321 cgctaaaaaa ggcagcattt ttgcactcat tctggcgcaa ttcgtcttcg tattgttggt
    46381 gtggggagct ggaaaggcgg cgacccaact ggcgcaaaat ggcagtgcgc tggcgcgtac
    46441 cagcctcggc agaggtttct ggctggctgc ggcgctggca ttgctggcct gtagcgatgc
    46501 catccgccga atctccacgc atccgctgtg gcgctggttg ttgcatatgc agattgccat
    46561 tattccgctg tggttgctgt actccggcac gcttaacgat ctctcactaa tgaaagaata
    46621 cgccaaccgt caggatgtgt ttgacgacgc gctggcacaa catctgacgt tgctgtttgg
    46681 tgcggtgctg cctgcgttag tgattggtgt gccgttgggc atctggtgct acttttccac
    46741 tgcgcggcag ggggcaattt tttctctgct caatgtcatt cagaccgtgc cttcggtggc
    46801 gctctttggc ctgttgattg cgccgcttgc cgcgctggtt acggcctttc cgtggctggg
    46861 gaagctcggc atagcaggaa ccggaatgac acccgcactg attgcgctgg tgctctatgc
    46921 cttgctgccg ctggtgcgag gcgtggtagt cggtttgaac cagatcccgc gcgatgtgct
    46981 ggagagcgcc agagcgatgg ggatgagcgg ggcgcagcgt ttcctgcatg ttcagttacc
    47041 gctggcgtta ctggtatttc tgcgcagcct gcgggtggtg atggtgcaaa ctgtaggtat
    47101 ggcggtgatt gcggcgttaa tcggcgcagg cggttttggt gcgctggttt tccaggggct
    47161 gctaagcagc gccattgatt tagtgttgct gggggtgatc ccggtaattg ttctggcggt
    47221 gcttaccgac gcgctgttcg atttgcttat cgcactgctg aaggtgaaac gtaatgattg
    47281 aatttagcca tgtcagcaaa ctgttcggcg cacaaaaagc cgttaacgat ctcaatctca
    47341 attttcagga agggagtttt tcggtgctga ttggcacatc tggctccggc aaatccacca
    47401 ccctgaaaat gattaaccgc ctggtggagc atgacagcgg agtgatccgc tttgccggag
    47461 aagaaattcg ctcgctgcca gtactggagt tgcgccgccg gatgggctat gccattcaat
    47521 ctattggcct gttcccccac tggagcgtgg cgcaaaacat cgctaccgtg ccgcaattac
    47581 aaaaatggtc gcgggcgcgg attgacgatc gtatcgacga attaatggcg ctactggggc
    47641 tggagtcaaa tttgcgtgag cgttatccgc atcagctttc cggtggtcag cagcaacgtg
    47701 tgggagtggc gcgtgcactg gctgccgatc cgcaagtctt actgatggat gaaccttttg
    47761 gcgcactgga cccggtaacg cgcggcgcgt tgcaacaaga gatgacgcgc attcaccgtt
    47821 tgctggggcg cactattgtg ctggtcactc atgatattga tgaggcgcta cggctggcag
    47881 aacatctggt attgatggat cacggtgaag tggtgcagca ggggaatccg ctgacgatgc
    47941 tgactcgtcc ggcgaatgat tttgtccgcc agttttttgg acgtagtgaa ctgggggtgc
    48001 acctgctttc gttacgtagc gtggcggatt acgtgcgtcg cgaagaacgg gcagaaggtg
    48061 aggcactggc agaagagatg acgctacgcg atgcgctctc cctgtttgtc gcgcggggat
    48121 gcgaggtgct gccggtggtg aacacgcagg gccagccttg cggcacgctg cattttcagg
    48181 atctgctggt ggaggcgtaa gcgtatgaag atgttgcgcg atccgctgtt ctggctcatt
    48241 gctctttttg tggcgctgat tttctggctg ccttacagcc agccgctgtt tgctgccttg
    48301 ttcccacaac tgccacgacc cgtttatcag caagaaagtt ttgcagctct ggcactggct
    48361 catttctggc tggtgggaat ttcgagtttg tttgcggtga tcattggcac aggtgccgga
    48421 attgctgtta ctcgcccgtg gggcgcggaa tttcgcccac tggtggaaac tattgccgcc
    48481 gttggacaga cttttccgcc tgtcgcagtg ctggcgattg ccgttccggt gatcggcttt
    48541 ggtctgcaac cagcgattat cgccttgatc ctttacggtg tgctgcccat cctgcaggtg
    48601 acacttgccg ggctgggagc gattgatccc agcgtgacag aagttgcgaa aggtatggga
    48661 atgagtcgag gtcagcgact gcgtaaggtc gagctaccgc tggcggctcc ggtgattctg
    48721 gcgggcgtgc gaacttcggt gattatcaac attggtacgg cgacgatcgc ctcaacggta
    48781 ggggccagta cgctgggtac gcccatcatc atcgggctta gcggatttaa taccgcgtat
    48841 gtgatccagg gggcgttact ggtggcactg gcggcgatca tcgcagaccg cctgtttgaa
    48901 aggctggtgc aggcgcttag ccagcacgca aaataaaggt ataacctgcg agcatgacgc
    48961 caccaattcc gcctaacgcc ataaacagga acagggcgat gaccccaatt ttagctatgc
    49021 gcataatgca ctccttatgt taacgaaagg attgtacagt aaagcgcatt tgttaacgaa
    49081 tcattaaata ccgagtggga aaatatcatg gccttgttct tgccaactgg tgagttgctg
    49141 ctgttgggcg gaggttcgat t
//
LOCUS       DAJTQY010000020        48550 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.020, whole genome shotgun sequence.
ACCESSION   DAJTQY010000020 DAJTQY010000000
VERSION     DAJTQY010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 48550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48550
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.020"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            <1..1224
                     /locus_tag="OO349_001179"
     CDS             <1..1224
                     /locus_tag="OO349_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR5866330.1"
                     /translation="FELPEITDNELLVSVISDSVCLSTWKAALLGSEHKRVPDDLENH
                     PVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSGYSAGYSYEYFGGNATYMI
                     IPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYHTTQYVYEHRMGVKPGGNI
                     ALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQVQKLLPVDLAASKGIELVY
                     VNTKGMSDPIQMLRALTGDVGFDDIFVYAAVPAVVEMADELLAEDGCLNFFAGLTDKN
                     FKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQLQPSFMVTHIGGLDAVPET
                     VLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKELARLVEETHGIWNEQAEK
                     YLLAQFGVDIGEAAQ"
     gene            1221..2186
                     /gene="yggF"
                     /locus_tag="OO349_001180"
     CDS             1221..2186
                     /gene="yggF"
                     /locus_tag="OO349_001180"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCR5866331.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPHTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            2208..2717
                     /gene="fumE"
                     /locus_tag="OO349_001181"
     CDS             2208..2717
                     /gene="fumE"
                     /locus_tag="OO349_001181"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HCR5866332.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDITWDFISNVNSITRNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            2714..3427
                     /locus_tag="OO349_001182"
     CDS             2714..3427
                     /locus_tag="OO349_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HCR5866333.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKLLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQALPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPAAALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(3399..4081)
                     /locus_tag="OO349_001183"
                     /pseudo
     CDS             complement(3399..4081)
                     /locus_tag="OO349_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
     gene            complement(4075..4752)
                     /locus_tag="OO349_001184"
     CDS             complement(4075..4752)
                     /locus_tag="OO349_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HCR5866334.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPREITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVIIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(4740..5123)
                     /locus_tag="OO349_001185"
     CDS             complement(4740..5123)
                     /locus_tag="OO349_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCR5866335.1"
                     /translation="MQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRA
                     RGVPLDEGWYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADS
                     TLQRVARYTMILLMLTEFGAWIWLR"
     gene            complement(5139..5447)
                     /locus_tag="OO349_001186"
     CDS             complement(5139..5447)
                     /locus_tag="OO349_001186"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5866336.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLPEWLSGTPRSPERWSHAITLWLLILAIVSTSQL
                     "
     gene            complement(5448..6026)
                     /locus_tag="OO349_001187"
     CDS             complement(5448..6026)
                     /locus_tag="OO349_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCR5866337.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVSGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(6049..6480)
                     /locus_tag="OO349_001188"
                     /pseudo
     CDS             complement(6049..6480)
                     /locus_tag="OO349_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            6852..7871
                     /gene="epd"
                     /locus_tag="OO349_001189"
     CDS             6852..7871
                     /gene="epd"
                     /locus_tag="OO349_001189"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HCR5866338.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            7921..9084
                     /gene="pgk"
                     /locus_tag="OO349_001190"
     CDS             7921..9084
                     /gene="pgk"
                     /locus_tag="OO349_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HCR5866339.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            9299..10378
                     /gene="fbaA"
                     /locus_tag="OO349_001191"
     CDS             9299..10378
                     /gene="fbaA"
                     /locus_tag="OO349_001191"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HCR5866340.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            10569..11429
                     /gene="mscS"
                     /locus_tag="OO349_001192"
     CDS             10569..11429
                     /gene="mscS"
                     /locus_tag="OO349_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HCR5866341.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            11568..12203
                     /gene="argO"
                     /locus_tag="OO349_001193"
     CDS             11568..12203
                     /gene="argO"
                     /locus_tag="OO349_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HCR5866342.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            12296..13036
                     /locus_tag="OO349_001194"
     CDS             12296..13036
                     /locus_tag="OO349_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HCR5866343.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            13203..14153
                     /locus_tag="OO349_001195"
     CDS             13203..14153
                     /locus_tag="OO349_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5866344.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATIFSILLKIVTGQTINPASSRLQLNYGVSRRRG"
     gene            complement(14095..15573)
                     /gene="scpC"
                     /locus_tag="OO349_001196"
     CDS             complement(14095..15573)
                     /gene="scpC"
                     /locus_tag="OO349_001196"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HCR5866345.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPAAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSSLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(15597..16373)
                     /gene="scpB"
                     /locus_tag="OO349_001197"
     CDS             complement(15597..16373)
                     /gene="scpB"
                     /locus_tag="OO349_001197"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HCR5866346.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEDFTLQMAHHISEKAPLAIAVIKEELRVLGEAHT
                     MNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(16384..17379)
                     /gene="meaB"
                     /locus_tag="OO349_001198"
     CDS             complement(16384..17379)
                     /gene="meaB"
                     /locus_tag="OO349_001198"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HCR5866347.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVAQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRGLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYHQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(17372..19516)
                     /gene="scpA"
                     /locus_tag="OO349_001199"
     CDS             complement(17372..19516)
                     /gene="scpA"
                     /locus_tag="OO349_001199"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HCR5866348.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTIADGIEYIKAAISAGLKIDDFAPRLSLF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCHTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(19720..20613)
                     /gene="argP"
                     /locus_tag="OO349_001200"
     CDS             complement(19720..20613)
                     /gene="argP"
                     /locus_tag="OO349_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HCR5866349.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            20996..21655
                     /gene="rpiA"
                     /locus_tag="OO349_001201"
     CDS             20996..21655
                     /gene="rpiA"
                     /locus_tag="OO349_001201"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HCR5866350.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            21911..23143
                     /gene="serA"
                     /locus_tag="OO349_001202"
     CDS             21911..23143
                     /gene="serA"
                     /locus_tag="OO349_001202"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCR5866351.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            23372..23431
                     /gene="ibsC"
                     /locus_tag="OO349_001203"
     CDS             23372..23431
                     /gene="ibsC"
                     /locus_tag="OO349_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HCR5866352.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            23764..23823
                     /locus_tag="OO349_001204"
     CDS             23764..23823
                     /locus_tag="OO349_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCR5866353.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(23924..24526)
                     /locus_tag="OO349_001205"
     CDS             complement(23924..24526)
                     /locus_tag="OO349_001205"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HCR5866354.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQLEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(24547..24730)
                     /gene="ssrS"
                     /locus_tag="OO349_001206"
     ncRNA           complement(24547..24730)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="OO349_001206"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24772..25101)
                     /gene="zapA"
                     /locus_tag="OO349_001207"
     CDS             complement(24772..25101)
                     /gene="zapA"
                     /locus_tag="OO349_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HCR5866355.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            25269..25847
                     /gene="ygfB"
                     /locus_tag="OO349_001208"
     CDS             25269..25847
                     /gene="ygfB"
                     /locus_tag="OO349_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HCR5866356.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            25873..27198
                     /gene="pepP"
                     /locus_tag="OO349_001209"
     CDS             25873..27198
                     /gene="pepP"
                     /locus_tag="OO349_001209"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HCR5866357.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     NVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            27195..28373
                     /gene="ubiH"
                     /locus_tag="OO349_001210"
                     /gene_synonym="visB"
     CDS             27195..28373
                     /gene="ubiH"
                     /locus_tag="OO349_001210"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HCR5866358.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVMLHCPDRVANVARTQSHVEVTLESGEMLTGRVLIA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGQITHAGKRSAYPLALTRAARP
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            28396..29598
                     /gene="ubiI"
                     /locus_tag="OO349_001211"
                     /gene_synonym="visC"
     CDS             28396..29598
                     /gene="ubiI"
                     /locus_tag="OO349_001211"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HCR5866359.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAVDA
                     LPQLRVSAINAASEKLLTRLGVWQDILARRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPTELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGANPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(29672..29755)
                     /locus_tag="OO349_001212"
     CDS             complement(29672..29755)
                     /locus_tag="OO349_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5866360.1"
                     /translation="MSKNTKSKNHGIRKYNAKTEVKLVYFK"
     gene            30046..31140
                     /gene="gcvT"
                     /locus_tag="OO349_001213"
     CDS             30046..31140
                     /gene="gcvT"
                     /locus_tag="OO349_001213"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HCR5866361.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKRGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQVGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPTDRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            31164..31553
                     /gene="gcvH"
                     /locus_tag="OO349_001214"
     CDS             31164..31553
                     /gene="gcvH"
                     /locus_tag="OO349_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HCR5866362.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            31672..34545
                     /gene="gcvP"
                     /locus_tag="OO349_001215"
     CDS             31672..34545
                     /gene="gcvP"
                     /locus_tag="OO349_001215"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HCR5866363.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASNVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWTEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            34812..35554
                     /locus_tag="OO349_001216"
                     /pseudo
     CDS             34812..35554
                     /locus_tag="OO349_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708665.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(35611..37044)
                     /gene="bglA"
                     /locus_tag="OO349_001217"
     CDS             complement(35611..37044)
                     /gene="bglA"
                     /locus_tag="OO349_001217"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HCR5866364.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDSLFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKFVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHDNPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLCGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGEKL"
     gene            37089..37399
                     /locus_tag="OO349_001218"
                     /pseudo
     CDS             37089..37399
                     /locus_tag="OO349_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
     gene            37563..38222
                     /locus_tag="OO349_001219"
     CDS             37563..38222
                     /locus_tag="OO349_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HCR5866365.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(38418..39398)
                     /gene="ygfZ"
                     /locus_tag="OO349_001220"
     CDS             complement(38418..39398)
                     /gene="ygfZ"
                     /locus_tag="OO349_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HCR5866366.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSVSRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            39641..39907
                     /gene="sdhE"
                     /locus_tag="OO349_001221"
     CDS             39641..39907
                     /gene="sdhE"
                     /locus_tag="OO349_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HCR5866367.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEIMVRLIQTRNRERGPVAI"
     gene            39888..40295
                     /locus_tag="OO349_001222"
     CDS             39888..40295
                     /locus_tag="OO349_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HCR5866368.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRCINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(40335..40856)
                     /gene="fldB"
                     /locus_tag="OO349_001223"
     CDS             complement(40335..40856)
                     /gene="fldB"
                     /locus_tag="OO349_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HCR5866369.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIIALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            40968..41864
                     /gene="xerD"
                     /locus_tag="OO349_001224"
     CDS             40968..41864
                     /gene="xerD"
                     /locus_tag="OO349_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HCR5866370.1"
                     /translation="MKQELARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            41889..42599
                     /gene="dsbC"
                     /locus_tag="OO349_001225"
     CDS             41889..42599
                     /gene="dsbC"
                     /locus_tag="OO349_001225"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HCR5866371.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKDMKEFLDEHQKMTSGK"
     gene            42605..44338
                     /gene="recJ"
                     /locus_tag="OO349_001226"
     CDS             42605..44338
                     /gene="recJ"
                     /locus_tag="OO349_001226"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HCR5866372.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLAPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            44429..45527
                     /gene="prfB"
                     /locus_tag="OO349_001227"
     CDS             join(44429..44503,44505..45527)
                     /gene="prfB"
                     /locus_tag="OO349_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HCR5866373.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            45537..47054
                     /gene="lysS"
                     /locus_tag="OO349_001228"
     CDS             45537..47054
                     /gene="lysS"
                     /locus_tag="OO349_001228"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HCR5866374.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(47097..47645)
                     /gene="idi"
                     /locus_tag="OO349_001229"
     CDS             complement(47097..47645)
                     /gene="idi"
                     /locus_tag="OO349_001229"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HCR5866375.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESSEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPRGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLRGIDATPWAFSP
                     WMVMQATNREARIRLSAFTQLK"
     gene            47700..47771
                     /locus_tag="OO349_001230"
     CDS             47700..47771
                     /locus_tag="OO349_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5866376.1"
                     /translation="MINQESVYRQPPVLSGCRQVKTI"
     gene            complement(47768..47893)
                     /locus_tag="OO349_001231"
     CDS             complement(47768..47893)
                     /locus_tag="OO349_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5866377.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(47895..>48550)
                     /gene="uacT"
                     /locus_tag="OO349_001232"
     CDS             complement(47895..>48550)
                     /gene="uacT"
                     /locus_tag="OO349_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HCR5866378.1"
                     /translation="IVFIESMGMFLALGEIVGRKLSSHDIICGLRVDGVGTMIGGTFN
                     SFPHTSFSQNVGLVSVMRVHSRWVCISSGIILILFGMVPKMAVLVASIPQFVLGGAGL
                     VMFGMVLATGIRILSRCNYTTNRYNLYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLH
                     SGIMLATLSAVVLNVFFNGYQHHADLVKESVSDKDLEVRTVRMWLLMRKLKKNEHGE"
BASE COUNT        11645 a        12175 c        13110 g        11620 t
ORIGIN      
        1 tttgaactgc cagaaattac cgataatgaa ctgttagtga gtgtaatttc tgacagcgtc
       61 tgtttatcga cctggaaagc ggcgttactc ggtagtgaac ataaacgcgt acccgacgat
      121 ttagaaaatc atccggtcat taccgggcat gaatgtgccg gggttattgt cgaagtgggt
      181 aaaaatctca ctggcaaata taaaaaaggc cagcgttttg tattgcaacc ggcgatgggg
      241 ttaccaagcg gatattcagc gggctacagc tacgaatatt ttggcggcaa tgccacttat
      301 atgattattc ccgaaatcgc cattaatttg ggctgcgtat taccgtatca cggctcttat
      361 tttgctgcgg cgtcgctggc agagcctatg tgctgcatta ttggtgctta tcatgccaat
      421 tatcacacca cgcaatatgt ttatgagcat cgcatgggcg tcaaacctgg cggcaatatt
      481 gcactgctgg cgtgtgcggg tccgatgggc attggcgcta tcgattacgc cattaacggt
      541 ggcatacaac cgtcacgggt ggtggtggtc gatatcgacg acaaacgcct ggcgcaggtg
      601 cagaagctgc tgccggtgga tctggcggcc agtaaaggca ttgagctagt gtatgtgaat
      661 accaaaggga tgagcgatcc tatccagatg ctgcgggcgc tgacaggaga tgtcgggttc
      721 gatgacattt ttgtttatgc ggcggtgcct gctgtcgttg agatggctga cgaattactg
      781 gcggaagatg gctgtctgaa cttctttgcc gggctgacgg ataaaaactt caaagtgccg
      841 tttaatttct acaacgtcca ttacaacagc acgcacgtag tcggtacatc cggcggttca
      901 acggacgaca tgaaagaggc gattgccctt agcgccacag gacagttaca gccgtcgttt
      961 atggtgaccc atattggtgg cctggatgcg gtgccagaaa ccgtgctcaa tctgccggat
     1021 atccctggcg gtaaaaaact catttataac ggcgtgacca tgccgctcac tgccattgcc
     1081 gattttgccg aaaaaggcaa aaccgatccg ctgtttaaag agttggcgcg gctggttgag
     1141 gaaacgcacg gcatctggaa tgaacaggcc gagaaatatc tgctggcaca atttggcgtt
     1201 gatatcgggg aggccgcgca atgatgtccc tggcgtggcc attatttcgc gttacggaac
     1261 aggcagcgtt ggctgcctgg ccgcacaccg gatgtggcga caaaaataaa attgatggcc
     1321 tggcggtcac cgcgatgcgc caggcattaa acgacgtcgc ttttcgtgga cgagtggtta
     1381 tcggcgaagg agaaattgac catgcgccga tgctgtggat tggcgaagag gtgggcaaag
     1441 gcgatgggcc agaagtcgat atcgcggttg atcccattga aggcacgcgg atggtggcga
     1501 tggggcagag caatgcgctg gcggtcatgg ctttcgctcc tcgagatagc ctgttacatg
     1561 cgcccgatat gtatatgaaa aagctggttg ttaatcgact ggctgccggg gcgattgatc
     1621 tgtctttgcc tctggcggac aacttgcgta acgtggcgcg ggcgttaggt aaaccgctgg
     1681 acaagctgcg catggttacg ctggataaac cgcgtctgag tgccgcgatt gaagaagcaa
     1741 cacagctggg cgtgaaggtt tttgccctgc cggatggcga tgtcgccgcc agcgtgctga
     1801 cctgctggca ggataatcct tacgatgtga tgtacaccat cggcggtgcg ccagaaggcg
     1861 tgatttctgc ctgtgccgtt aaagcgttag gcggggatat gcaggccgag ctgattgact
     1921 tttgtcaggc taaaggggat tacacggaaa atcggcagat tgcagagcag gagcgcaagc
     1981 gttgtaaggc aatgggcgtt gacgttaacc gtgtgtactc gctcgatgaa ctggtgaggg
     2041 ggaacgatat cctctttagc gctacgggcg tgacgggcgg cgagttggtg aacggtatcc
     2101 aacagacggc gaatggggtg cggacgcaga cattactgat cggcggcgcg gaccaaacgt
     2161 gtaatataat agactccctg cattgatggt gatctatcgg aagaccaatg gcgacgctga
     2221 cagaagatga tgtgcttgag caactggatg cacaggacaa tttattttca tttatgaaaa
     2281 ctgcgcattc tattttgctc caggggatac gccagtttct gccgtcgctg tttgtcgata
     2341 acgatgaaga gatcgtcgaa tatgcagtga agccgttact cgcccaaagc ggcccgcttg
     2401 acgatattga tgttgcgctg cgtttgattt atgcgctggg gaaaatggat aaatggctgt
     2461 atgccgatat cacgcacttt tcccagtact ggcattacct gaacgaacag gatgaaacgc
     2521 ccggatttgc cgatgatata acctgggatt ttatcagcaa tgtcaacagt ataactcgca
     2581 atgcgacgct ctacgatgcg ttaaaagcga tgaagttcgc cgatttcgcc gtctggtcag
     2641 aggcgcgttt tagcggaatg gtcaaaacgg cgctgacgct ggcagtaacg accaccttaa
     2701 aggaattaac gccgtgaaaa ttgaattaac ggtgaatggg ctgaacgtgc aagcgcagta
     2761 ccatgatgaa gagatagaac gcgtgcataa actgttgctg cgtatgctgg cggccttaca
     2821 gacggtaaat ccgcagcggc gtacggtggt ttttctttgc gctccgccgg ggacgggtaa
     2881 atctacccta accaccttct gggaatatct cgcgcaacaa gacccggagc tgcccgctat
     2941 tcaggcgctc ccgatggatg gttttcacca ttacaatagc tggctggacg cgcatcaatt
     3001 gcgccccttc aaaggcgcac cagagacatt tgacgttgcg aaactggcgg aaaatctgcg
     3061 ccaggtggtg gaaggggatt gtacgtggcc gcagtacgat cgacaaaagc atgatcctgt
     3121 tgaagatgcg ttgcacgtta ccgcaccact cgtcatcgtc gaaggaaact ggttgttgct
     3181 ggacgacgag aagtggtgcc agcttgcgca attttgtgat ttctcgatct ttatcaacgc
     3241 gccagctgct gcgttgcggg agcgtctggt ggggcgcaag ctggcgggag gggtatcgct
     3301 ggcagatgca gaagcctttt atgaccgtac cgacgggccg aatgttcgcc gggtgctgga
     3361 agagagtctg cctgcaaatt tgaccttaat gatgaccgct acgggtgaat atcgtctggt
     3421 ggattaacgt tcctgattac accatcttcc agccgcacca cacgagagaa atgacgccgg
     3481 gtaaatgcgg cgtcatgact gattgctacc acgctcgtgc ctcgctggcg gcacttctcc
     3541 agccagcttt caaacacgct caaccaattt tcgtcaaaat cccgactcgg ctcgtccagc
     3601 agtaatagtg gcggagagag tgcttccagg caggcgacgg cgaccatccg tcgctgcgca
     3661 ctatgcagat ccaacgggtg cgagttggca gtgtctgcta actgacaaca ttgcagggca
     3721 gcatgggtac gttgagtgat ttcatccgcc gggcattttt gcagtttcaa accaaatgcg
     3781 atctcgtcag cgacggtgct gtgaaaaagt tggttttccg cttcctgaaa cagaacagta
     3841 cgccaacctt tgccgcgcgc tggcggtttt tcaggttttt catcgcctgt tgttgcagca
     3901 taaccgtacc ggctgtgggc gtaagaagac ctgccattac tcgtaacagc gtcgatttcc
     3961 ctgcgccatt gtcaccggtc agcgccaacc attcaccttg tttgagttgc agagaaatgt
     4021 cgcaaagaca atccgttgcc gcgccaggcc agcgataaga tatcttattt aacgttaaca
     4081 tagtggctgg agcgctccgt tttgcaattg ccacgcttgc tggcagtagt tcaggaaagg
     4141 tgtgcgatga cgttcaaaaa gaataatcag cgaaccacgt tcaaacgccc agtgttgtaa
     4201 gcgctgcaac agcatttcgc tggcctgcgg cgtcaggcgg ctaaaggctt catcaagaat
     4261 taagagcttt ggctgcatcg cgatggcgca ggcgatgatc acgcgctgtg tctctccacc
     4321 ggagagtgtt gccgggtgac gatggcgcaa cggctggcac tcggtcagag tcagggcggc
     4381 atcaatccgc gccataatct ctgcttcagc aagacacaga ttttctggcc caaaggcaac
     4441 ttcttcttcc acgctaaagg tacagccgga gagctgcaaa tagggcgatt gctggacgag
     4501 ctggatggtg gcggattgtt cattaagcgg gagatgccca atgggcgtac cgaggagcgt
     4561 ccccgtgcct gttatctctc ttggcagaaa gtccggatac cagcccgcca tcagctgtgc
     4621 cagcgtactt ttgccactgc cgttgtcacc aaagatggcg accatccccg gtttgacata
     4681 gtgaaagtca taacagaacg gaggatgcgt tgagtgtgtc gggcaataac ggaactgttc
     4741 taacgtaacc atatccacgc tccgaattct gtcagcatca gcaagatcat ggtgtaacgc
     4801 gctacgcgtt gtagggtgct atcggctggt gcccacaacg ttgttcgtcg gttgtggatc
     4861 cgaaatgccc gcatatccag cgccgcacca cggacagcca gatcgttaag cgcattgtgt
     4921 gttagcggaa taattaacgc gggcatagca cgtaaacgct gataccagcc ctcatccagc
     4981 ggcacgccgc gggcacgctg cgcttcatga ataatcgcta attgccgttt taactgctca
     5041 acgaccaata acggcccggc aaacagataa gcgacgcctg gcggcaggcg agaagcaaac
     5101 agagcgcgaa taaaacattg cacggggacg tactgcattc acaactgtga ggttgaaaca
     5161 atcgccagaa tcagcaacca gagcgtaata gcatgcgacc accgttctgg cgagcgtggc
     5221 gtgccgctta gccattctgg tagccagcca ccgtgtacca gccacagccc ggctcccagc
     5281 gagaacatca gccagacgac atattttgcc cgccgccgcg tggcctttag cgcaataagg
     5341 cagaaaaagg tcgccgcgct atagatcggc aaaatggtct gcgcgggcag gataagcgtg
     5401 gtgcaggccg ccaatgccca cagtgttaat gaggtaaatg gatgcattta gcgcacggca
     5461 gccattgccg gaaaatggcg cgtggtgcga atcggcagct ggcgcagtaa aagccagaca
     5521 atcaccgctg taagaatttt atcgaccagg ttcgcgccta tcaccgtaat cgccaccgac
     5581 tccaccaggt tttgccccat cgagtgcatc caggcaacga acaaatcggc tccgctaccg
     5641 gtgacaccgc cgaacagcgc agtacgcagc ggtacagcga cgaccgttac cgcaagcgta
     5701 ataatcacgc cgctgacaac gacttttggc agggtgcgaa accaacccgc tcgcgccagc
     5761 caaccggaga ccaggccgat gaccatcgcg acgggtgcaa atgcggcggc gatcgggtcg
     5821 gtcagcaatc cccagagtaa attggtcagt aagccggtca gcattccgat caccggacca
     5881 agcagtaccg cgctaataag tgtgccgatg gagtcgagaa atataggtaa tttgaccatg
     5941 ctggccagtt gcccgccgat catattgatg gcgatagata taacgatcaa caccagtgcc
     6001 tgactggaaa agtggctacg cgccataaga aaacctcttt gaaagtagtt atttaacggg
     6061 ggaaatatgc aactcgtcgt aaccagaaag cgcgcaaggt tggcggctaa acgccagttc
     6121 ctcaaggcta ccgatcacca gttcaagcgt ggtccgcacg gtacagccgg gcatcatatg
     6181 accgccaagc atcgtgccgt gtggatcgga aacgcagaga tgaagatgct caccactctg
     6241 ttccagcgtg ccgtttagtg agatcacttc gaatgtaccg ttcaggagcg tggtgccttc
     6301 ttgcccggca tagcgcaagg cgacgtcagt caggctaccg gtacagcctg caatctaggc
     6361 ggcatgaagt tgatgttgct gtgcaaaagc gtgtagctgg gaaaaaactt cctgcccggg
     6421 gaggaggcgc agtgcgtaaa agcgtgcgga ggaagaatat gagtagggca atgtcatcat
     6481 tggagtactc cgaaactcgg acgtattgcg gataatatcg acgctgcatc agtcatattc
     6541 tgatataagt cagggtttgt gacggcacaa cgaaaagatt ttgctacgct tattcgtgat
     6601 tctgcaaagt ggaataaatt ccacaggggc ggggctgaaa acgtgacgta agtcagataa
     6661 ttgctttctt tgactggaca atcattcctt ttattccacg tttcgcttat cctagctgaa
     6721 gcgtttcagt cgattaaatg ttcgacaatt aaccaatcag tcgcagtttg cgacaggtaa
     6781 ggtttccccg gacgatttgc tggattactc tgtctggcaa atttgctcag ggaaaacctt
     6841 gcaggagatc tatgaccgta cgcgtagcga taaatggctt cggtcgcatc gggcgtaatg
     6901 tggttcgtgc tttgtatgaa tccggacgcc gggcggaaat taccgtggtg gcaatcaacg
     6961 aactggcgga tgctgcgggc atggcgcatt tgttgaaata tgacaccagc catggccgtt
     7021 ttgcatggga agtacgacag gaacgcgatc aactttttgt tggtgatgac gccatccgcg
     7081 tattgcatga acgttcactg caatcgctcc cctggcgtga acttggcgtt gatgtagtcc
     7141 tcgactgcac cggcgtatat ggctcccgcg agcatggcga agcgcatatt gccgccgggg
     7201 ccaaaaaagt gctcttttca catcctggca gtaacgatct cgacgcgacc gttgtttacg
     7261 gcgtcaatca ggatcaactt cgtgcggaac accgcatcgt ttctaacgct tcctgtacca
     7321 cgaattgcat aattcccgtc atcaaattgt tagatgatgc gtacggtatt gagtccggca
     7381 ctgtgaccac aattcactcc gccatgcacg atcaacaggt tattgatgca taccatcctg
     7441 acctgcgtcg cacccgggca gccagccagt cgatcattcc ggtcgatact aaactggccg
     7501 ctggtatcac acgatttttt ccgcaattta acgatcgctt tgaagcgatt gcggtacgtg
     7561 tgccaaccat aaatgtgacg gcaatcgatt taagcgtgac ggtgaagaaa cctgtaaaag
     7621 ccaatgaagt caacctgttg ctgcaaaaag cagcacaagg tgcatttcat ggtatagttg
     7681 actatacgga attgccgttg gtctctgtag attttaacca cgatccgcac agtgccattg
     7741 tcgatggcac ccaaacccgg gtcagtggcg cacacctgat caaaacgttg gtctggtgcg
     7801 ataacgaatg gggctttgct aaccgaatgc tcgacacgac gttagctatg gctactgttg
     7861 ctttcaggta agacgcaagc agcgtctgca aaacttttag aatcaacgag aggattcacc
     7921 atgtctgtaa ttaagatgac cgatctggat cttgctggga aacgtgtatt tatccgtgcg
     7981 gatctgaacg taccagtaaa agacgggaaa gtaaccagcg acgcgcgtat ccgtgcttct
     8041 ctgccgacca tcgaactggc cctgaaacaa ggcgcaaaag tgatggtaac ttcccacctg
     8101 ggtcgtccta ccgaaggcga gtacaacgaa gaattctctc tgctgccggt tgttaactac
     8161 ctgaaagaca aactgtctaa cccggttcgt ctggttaaag attacctcga cggcgttgac
     8221 gttgctgaag gtgaactggt tgttctggaa aacgttcgct tcaacaaagg cgagaagaaa
     8281 gacgacgaaa ccctgtccaa aaaatacgct gcactgtgtg acgtgttcgt aatggacgca
     8341 ttcggtactg ctcaccgcgc gcaggcttct actcacggta tcggtaaatt cgctgacgtt
     8401 gcgtgcgcag gcccgctgct ggcagctgaa ctggacgcgc tgggtaaagc actgaaagaa
     8461 cctgctcgcc cgatggtggc tatcgttggt ggttctaaag tatctaccaa actgaccgtt
     8521 ctggactccc tgtctaaaat cgctgaccag ctgattgttg gtggtggtat cgctaacacc
     8581 tttatcgcgg cacaaggcca cgatgtgggt aaatccctgt acgaagctga cctggttgac
     8641 gaagctaaac gtctgctgac cacctgcaac atcccggttc cgtctgatgt tcgcgtagca
     8701 accgagttct ctgaaactgc accggctacc ctgaaatctg ttaacgatgt gaaagctgac
     8761 gagcagatcc tggatatcgg tgatgcttcc gctcaggaac tggctgaaat cctgaagaat
     8821 gcgaaaacca ttctgtggaa cggtccggtt ggcgtgttcg aattcccgaa cttccgcaaa
     8881 ggtactgaaa tcgtggctaa cgctatcgca gacagcgaag cgttctccat cgctggcggc
     8941 ggcgacactc tggcagcaat cgacctgttc ggcattgctg acaaaatctc ctacatctcc
     9001 actggcggcg gcgcattcct cgaattcgtg gaaggtaaag tactgcctgc agtagcgatg
     9061 ctcgaagagc gcgctaagaa gtaaaaaatc acagggcagg gaaacctgca cttgtttcag
     9121 cgcgctttta gagcacgcat tttttcatac tctaaataat tcgagttgca ggaaggcgac
     9181 aagagagtga atccccagga gcttacataa gtaagtgact ggggggagcg aacgcagacg
     9241 cagcacatgc aacttgaagt attatgagta taaggcccga cgatacagga caagagacat
     9301 gtctaagatt tttgatttcg taaaacctgg cgtaatcact ggtgatgacg tacagaaagt
     9361 tttccaggta gcaaaagaaa acaacttcgc actgccagca gtaaactgcg tcggtactga
     9421 ctccatcaac gccgtactgg aaactgctgc taaagttaaa gcgccggtta tcgttcagtt
     9481 ctccaacggt ggtgcttcct ttatcgctgg taaaggcgtg aaatctgacg ttccgcaggg
     9541 tgctgctatc ctgggcgcga tctctggtgc gcatcacgtt caccagatgg ctgaacatta
     9601 tggtgttccg gttatcctgc acactgacca ctgcgcgaag aaactgctgc cgtggatcga
     9661 cggtctgctg gacgcgggtg aaaaacactt cgcagctacc ggtaagccgc tgttctcttc
     9721 tcacatgatc gacctgtctg aagaatctct gcaagagaac atcgaaatct gctctaaata
     9781 cctggagcgt atgtccaaaa tcggcatgac tctggaaatc gaactgggtt gcaccggtgg
     9841 tgaagaagac ggcgtggaca acagccacat ggacgcttct gcactgtaca cccagccgga
     9901 agacgttgat tacgcataca ccgagctgag caaaatcagc ccgcgtttca ccatcgctgc
     9961 gtccttcggt aacgtacacg gtgtttacaa gccgggtaac gtggttctga ctccgaccat
    10021 cctgcgtgat tctcaggaat atgtttctaa gaaacacaac ctgccgcaca acagcctgaa
    10081 cttcgtattc cacggtggtt ccggttctac tgctcaggaa atcaaagact ccgtaagcta
    10141 cggcgtagtg aaaatgaaca tcgataccga tacccaatgg gcaacctggg aaggcgttct
    10201 gaactactac aaagcgaacg aagcttatct gcagggtcag ctgggtaacc cgaaaggcga
    10261 agatcagccg aacaagaaat actacgatcc gcgcgtatgg ctgcgtgccg gtcagacttc
    10321 gatgatcgct cgtctggaaa aagcattcca ggaactgaac gcgatcgacg ttctgtaagt
    10381 cattcctttc tgcttatctc aaggcccgct ctgcgggtct ttttttcgcc aaaagcaagg
    10441 tgattcagat gagaaatctg tgatctattt ggcaaaatta tgctttattg tttacccttg
    10501 tcagactgcc cgtcataagg cggcggagtg tatttctcca ttttgagtca gttgaaaagg
    10561 aatattgaat ggaagatttg aatgttgtcg atagcataaa cggcgcggga agctggctgg
    10621 tagctaacca ggcgctgctg ctaagttatg cagtaaacat cgtggcggca ctcgcgatca
    10681 tcatcgttgg tttgattatc gcgcggatga tttccaacgc ggtgaatcgc ctgatgatct
    10741 cccgtaaaat cgatgccact gttgctgatt ttctttctgc attagtccgt tacggtatta
    10801 tcgcctttac gctaatcgct gccctgggac gcgtgggtgt acaaacagcg tcagtcattg
    10861 ctgtactcgg tgccgcaggc ttagctgttg gtctggcttt gcaggggtca ctttctaacc
    10921 tggccgctgg cgtgttactt gtcatgttcc gcccgttccg tgccggagaa tatgttgacc
    10981 tcggcggcgt agccggtact gtgctgagtg tgcagatttt ctccaccacc atgcgtactg
    11041 cagacggtaa aattatcgtt attccgaacg gtaaaattat tgccggaaat attattaact
    11101 tctcccgcga gccagttcgc cgtaacgaat ttattattgg cgtggcgtat gattccgata
    11161 tcgatcaggt taagcagatc ctgaccaata ttatccagtc tgaagatcgc attttgaaag
    11221 atcgcgaaat gactgtgcgc ctgaacgaac ttggtgcatc gtcgattaat ttcgtggtcc
    11281 gcgtctggag caacagcggc gatctgcaaa acgtgtactg ggatgtgctg gagcgtatta
    11341 aacgtgaatt tgatgccgcc ggtatcagct tcccgtaccc gcaaatggat gtgaacttta
    11401 agcgggtgaa agaagacaaa gctgcgtaat caacgctgag gccagataat actctctggc
    11461 ctctctctta ttagttattc tgattgccaa ttaatattat caatttccgc taataacaat
    11521 cccgcgatat agtctctgca tcagatactt aattcggaat atccaacgtg ttttcttatt
    11581 actttcaagg tcttgctctt ggggcagcta tgattctgcc gctcggccca caaaatgctt
    11641 ttgtgatgaa tcagggcatt cgtcgtcagt accacattat gattgcctta ctttgcgcta
    11701 tcagcgattt ggtcctgatt tgcgcgggga tttttggtgg tagcgcgtta ttgatgcagt
    11761 cgccgtggtt gctagcgctg gtcacctggg gcggcgtagt cttcttgctc tggtatggtt
    11821 ttggcgcttt taaaacagcg atgagtagta atattgagtt agctagtgcc gaagtcctga
    11881 agcaaggcag atggaaaatt atcgccacca tgttggcagt gacctggctg aatccgcatg
    11941 tttacctgga tacttttgtt gtactgggca gccttggcgg gcagcttgac gtcgaaccaa
    12001 aacgttggtt tgcgctcggg acaattagcg cctctttcct gtggttcttt ggtctggcta
    12061 ttctcgcagc ctggctggca ccgcgcctgc gcacggcaaa atcacagcgc attatcaatc
    12121 tggtagtggg atgtgtcatg tggtttatag ccttgcagct ggcgagagac ggtattgctc
    12181 atgcacaagc cttgttcagt taggcgctat ctgatggaaa aataaaacag aggcgctaag
    12241 cttgcctcca gaggtcctga attttgggca agtcaattaa cacggagaga ctaacgtgaa
    12301 gttcaaagtt atcgccctgg cggcattaat gggtattagc gggatggcag cgcaggctaa
    12361 cgaattgccg gatggaccgc atattgtcac ctccggtacg gcaagtgtgg atgcggtgcc
    12421 agacattgcc actcttgcga ttgaagttaa cgtggccgcg aaggatgccg ctacagccaa
    12481 gaaacaggca gatgagcgcg tcgcacaata catttccttc cttgaactca atcagatcgc
    12541 gaaaaaagat atcagctcag cgaatttacg cacccagcca gattatgact atcaggatgg
    12601 taaaagtatc ctgaaaggct accgagctgt gagaacggtg gaagtcacgc tccgtcagtt
    12661 agacaaactg aattccttgc tggatggcgc gctgaaggcg ggtcttaacg aaattcgttc
    12721 tgtgtcgctg ggcgtggcgc agccggatgc ctataaagac aaagcgcgta aggcagcgat
    12781 tgataacgcg attcatcagg cgcaggaact ggcgaacggc tttcatcgta aactggggcc
    12841 ggtatatagc gtgcgctacc atgtttccaa ctatcagccc agcccaatgg tgcggatgat
    12901 gaaagccgat gccgcgccgg tgtctgccca ggaaacttac gagcaggccg ctattcagtt
    12961 tgatgatcag gtcgatgtgg tcttccagtt agaacctgtg gatcaacaac ccgctaaaac
    13021 acctgcagca caataatact tgtagcccga cgtattcgtc gggctaatgc ctttacccga
    13081 tattgcctcc tttttctgta attttctgta aaaccatgac gtaatcacct atctttactc
    13141 gcggtttcat caaggatagg ctgactgaaa cggttaagcg gacaaggatg cggggagggg
    13201 ggatggacat ttttatttcg aaaaagatgc gcaatttcat tttattagcg caaaccaata
    13261 atattgcccg ggcggcagaa aaaatccata tgacggcttc gccatttggt aaaagcattg
    13321 ccgcgctgga agaacaaatt ggctatacgc tatttacccg caaagataat aatatcagcc
    13381 tcaataaagc cggtcaggaa ctgtatcaaa aactgttccc ggtttatcaa cgactttctg
    13441 cgattgataa tgaaatccat aactcagggc gtcgttcacg agaaattgtg attggcatag
    13501 ataatactta tccgacaatt attttcgatc agttgattag tctcggagat aagtatgaag
    13561 gcgtgactgc ccagccagtt gagttcagcg aaaacggcgt tatcgataat ctttttgacc
    13621 gtcagttaga ttttattatc tccccacagc atgtatctgc acgagttcag gaactggaaa
    13681 acctgactat cagtgaatta ccaccgctgc gattgggctt tctcgtttct cgccgttatg
    13741 aagagcgtca agagcaagag ctgcttcagg agctgccctg gctacaaatg cgctttcaaa
    13801 accgggctaa cttcgaagca atgattgatg cgaatatgcg accttgtggg atcaatccca
    13861 ccattattta tcgtccctac agttttatgg ccaaaatcag cgccgtggag cgcggacatt
    13921 ttctgacggt gatcccgcat tttgcctggc gactggtgaa tccggcaacg ctgaaatatt
    13981 ttgatgcgcc gcacaggccg atgtatatgc aggaatatct ttattcaatc agaaatcatc
    14041 gttataccgc cacgatcttc agcatattgc tgaagatcgt gacgggacaa accattaacc
    14101 cagcatcgag ccggttgcaa ttaaattacg gtgtaagtcg aagacgtggc taagatcgtg
    14161 gtgaatatgt ccgccgggcg cattttccag atagcgatgc agataatccc gatacatagg
    14221 atgtgcacaa ttatcaatga tagtgcgggc gcgttgaagc ggggaaagac cgcgcagatc
    14281 ggcgatccct tgttcggtga tgatcacttt gacgctgtgt tcgctgtgat caacatggct
    14341 gcacattggc acgacggttg agatcttgcc ttctttagca atcgacggag ccataaagat
    14401 cgacagatac gcgttgcgtt caaaatcacc gctgccgccg atgccgttca tcagatcgac
    14461 cccggctacg tgcgttgaat ttgcatgccc gtaaatatca aactccaggc cgacgttcag
    14521 agcgatgacg cccagacgac ggatgatttc cgggttattg gaaatctcct gcggacgcaa
    14581 cacaatgcgg ctggcaaagt aatccatatt gtcgtaaatc ttgcgcaggg aatcggccga
    14641 gattgtcagg ctggaggcgc tggccccgct gattttgccg gtttccagta aatgcaccac
    14701 cgattcctgt agcacttccg aatacatcat gaacggagga atttccgggt tttcccccag
    14761 acgtgccatt accgcattat tgatattgcc cacgccactt tgcagcggca gaaattccgg
    14821 cggaatacgc ccatgcgcca tttcctgcaa taagaacgtg accacgttat cggcaatctg
    14881 ctggcacatg ggattttgct tatccagcat attaccggcg tcgggcaagt tggtttccac
    14941 gacggcgaca atctttttcg gatcgatttg cacatagcgg gtaccgacgc gatccattgc
    15001 atggaagatc gacacgctat tgcgccgcgg tggcgcgcca ggaatcacaa tatctgccag
    15061 ttctgcaacg cgcggatcgt gatagtgatt aagttcaatg atcactttct tcgctcgcag
    15121 cagccaggtc ggcgcattac cgatcccgct ggttaaccag actcgaccat ccggtgccag
    15181 tgccgatgct tcaatgacgg caacatcaat gtcgccgaag aaaccgtaat tgaccatttg
    15241 cgccacttcg ctcaaatgca ggtcaacgaa actcaccgcg ccctgattga tctttttacg
    15301 taaactggac gatgtttgat atggcgcacg ccaggaaaca gcatcggcgt cagaaagtac
    15361 atcgtcagcg gcggcgctga ttgacgcccc cgtcagcagg cgaatttgat acggcttttt
    15421 ggcctcatgc tgttcgttag ctctgcgggc aatcgcggcg ggtagggctt tcggcgaacc
    15481 cgctggggta aagccgctaa atgccaccat gtcgttatgc tggataattt ctgccgcttc
    15541 atcggcggtc atccttgtcc actgagtttc catttttact ccttcgttcg cagggattaa
    15601 tgaccaacga aattaggttt acgtttttcg aggaaagcgt tcatcccttc ctggtaatct
    15661 tcgctgtcat aaaccgcgcg gcgcatcccc tgaatacgtt caaattcatc ggagttcatg
    15721 gtgtgtgctt cgcccagtac acgcagctct tctttgataa cggcaatggc taacggcgct
    15781 ttctcagaga tgtggtgcgc catttgtaag gtgaaatctt ccacttccac aacatggttg
    15841 aggatgccga cagccagcgc gcgctgggcg gtgattggcg aagcggtaaa aatcagctct
    15901 ttgacaatgt ggaagcccgc gtcgcgggtc aggttgtgaa tgccgaccag gttatacggg
    15961 acgccgaggt ttacaggcgt cattgagaag gttgaggtac tggcggcgat gatcagatcg
    16021 gaactcatga tcatttcaaa tgcgccaccc caaacactac cttccaccat cgaaatgatc
    16081 ggtttcggga atttttggat catgcgggtg atttgacgca atggatcatc ataggagagc
    16141 ggatcgcgac cgccagacgg cagttcgtga atatcgtgac ctgcggagaa gactttggat
    16201 ccacttggcg cccgcaaaat gatacagcga atttccggcc ggttgagatc gcttaacgcc
    16261 tgcataagat catcaataaa gactttactt aaggcattaa gttttcgtcc atagttaaac
    16321 tcaatgaccg ccactttgtt gatagtggca acgttcacat actgataaga cataaaaatt
    16381 cctttaatca aaatattgcg tctggataaa ttcactgagc tgccgcaggc cggtgcgcgg
    16441 tgagagcgta ttgtttttga ccgctaaaag cgtctggtgg taatagcgat cgaaatcttc
    16501 attcgcgaac aggtgattca gtacttcttc ttcggtctgc ttacgcagcc attccaccga
    16561 ttgttgttgc cgcacttgtt gtaaacgacc actggcagtt agcgcggttt tgaagtcgat
    16621 gatggcgtgc cagatctcat cgattccacg tttttccagt gcgctacaag tcagaccccg
    16681 tggctgccat tcgtcgtatt tacgtcgcag aatatgcagg gcactctcgt acatatgccg
    16741 ggcaatggcg acattggtat ggttatcgcc atcgtctttg ttgataacga gcagatcagc
    16801 cacttccatc agcccttttt taatgccctg cagatcatcg ccgccaccgg caatttgcaa
    16861 cgagataaaa cagtccacca tgcgggcgac ttccgtttcc gactgcgcga cgccaaccgt
    16921 ttcgacaatc actacgtcat aacccgccgc ttcgcataac agcattaatt cccgtgctcg
    16981 ctgactggca ccgcccagat gaccggagga gggtaccggg cgaataaacg ccgcctcggc
    17041 acgcgccagg tcattcatgc gggttttatc cccgagaatg ctaccgccag tgaccgggct
    17101 gctgggatcg accgcaataa cagcaacctt taatccctca cgaatcaaca acatgccaaa
    17161 ggcttcaaga aaggtacttt tccccgcgcc gggtgtgccg gtaacgccca gtcgcagggc
    17221 gttaccacag aacggcataa tagcgtcaag cagctgtgta cttagcgcct gatgacgtgg
    17281 gtgacggctt tccaccagcg tcatggcttg ggcgagcgtg gcacgctcac cctgacgtaa
    17341 gcggcgaata ctttctgcca gcgtggcttc attaatcatg atgctggctt atcagattca
    17401 gtacgtcgcg cacactgtcg agcataggtg tacctggacc ataaatcgcc gccacgccgc
    17461 gctcttgcag gaaggcgtaa tcctgcggcg gaatgacgcc acccgcgacc acgcagatat
    17521 cttcgcgtcc ccattttttc agcgcttcga ccagttccgg gatcagcgtt ttatgaccgg
    17581 cagccagtga ggatgcgccc actacgtgaa cgtcgttttc tacggccagg cgggcgatct
    17641 cttcaggtgt agagaacatc gggcttaaat ctacgtcgaa accgagatcg gaataggcgc
    17701 tggcgatcac tttcgcgccg cgatcgtgtc catcctggcc catcttagcg atcagaatgc
    17761 gcgggcgacg accattgtcg gcaaggaact gctccgtttg cgcaacaatg gcatcgaact
    17821 cggaggccga tttctcagac tgatgatagc tttgcgcaat cacgccggta acacactggc
    17881 ttggcaccag ataacggtcg aaagcgactt ccagcgcatc ggaaatttca cccagggtgg
    17941 cgcgaacgcg agcggcatta acagcggcag ccagcaggtt ttcgttatgc tgtgcggcgt
    18001 gagtcagggc gttcaacgcg gcggttacgg cggcatcatc acgggtggcg cgaatgcgtt
    18061 ccagcgaagc aatttgctcg ttacgcacca tcacgttgtc gatctcaagt acatcggttt
    18121 cgtcttcgtg atccagtttg tacttgttga caccaacgat gacacgcttg ccctggtcga
    18181 tcagcgactg ttcgcgcgct gaggcctctt cgatcattcg ttttggcaga cctgcttcga
    18241 tcgctttcgc catgccaccg gcttcgtcga tctgttggat aatagctctg gcttgtttga
    18301 cgatttgatc ggtcagcgac tcaatgtaat aggatccggc cagtggatcg acggtgtggc
    18361 agagttctga ttcttcctgg atgatgatct gggtgttgcg ggcaatgcgt gctgagaaat
    18421 cggtaggcaa accaagcgct tcgtcaaagg cgttggtatg cagtgactga gtaccgccca
    18481 gcgtcgcagc cagtgcttca atggtggtgc ggataacgtt gttatacgga tcctgttcag
    18541 tcaggctcca gcctgaggtc tggcagtggg tacgcagcgc cagtgatttc ggatcctgtg
    18601 cgccaaatcc actgaccgct tcgctccata aataacgtgc cgcacgcaac atggcgacgt
    18661 tcataaacag atccatgccg ataccgaaga ataacgacag gcgaggagcg aagtcatcaa
    18721 ttttcagtcc ggcagagatt gctgctttga tgtactcaat cccatcggcg atcgtaaatg
    18781 ctacctgctg cacgcagttg gcacccgctt cccccatgtg gtaaccgctg atactgatgg
    18841 tattaaatcg cggcatgttg ccggaacacc aggcgatgat gtcggcgata atgcgcattg
    18901 acggttttgg tgggtaaata taggtgttgc ggcagaggta ctctttgaga atatcgtttt
    18961 gaatggtgcc ggtcagttta tcaggtgtaa caccttgctc ttctgcggcg acgatataaa
    19021 acgccagtac tggtagcact gcgccattca tggtcatcga aaccgacatt ttatccagcg
    19081 ggatctggtc gaacaggact ttcatatctt ccacggtgtc gatagcgacg cccgctttgc
    19141 cgacgtcacc cgccacgcgt gggttatcgg agtcgtagcc acggtgggtg gcaaggtcaa
    19201 acgcaacgga aagacctttt tgcccggcgg ccaggttacg gcgataaaaa gcgttggact
    19261 cttttgctgt tgaaaaacca gcatactgac ggatggtcca cggttgggcg gtatacatag
    19321 tggcacgcgg gccacgaacg tagggcggca aaccaggaag ggtacctgtc acctccagat
    19381 tatcgagatc ggcttcggta tacagcggct tgatggcgat cccttccgcg gtttgctgaa
    19441 ccagcgagtc gacagttttc tcccgacggc tcaattcctt gttggcaagc tgttgccact
    19501 cctgctcgtt agacattatt tgctccgtaa aacgcaatca aaatgagggt atttgcgctt
    19561 acggtgagct ttttacgtgg gtttgtcgcc agcaaaaaaa atgcattcag acgtcgtttt
    19621 ttgccgcctg agatttgtct attttgtgat gtgattcaag gaatacaaaa agaagtgagg
    19681 acaacgcctg atatgtgcta tcaggcatta tttgatggat taatcctgac gaaggacttt
    19741 gtgaccataa tcgagtaacg catcagtgac tttacgcatc atgcggcttt caggagcaaa
    19801 gcggtgccag tagagcatcc gtcgttgaaa tagcccaggc gttaagtcaa tcagttcacc
    19861 gctggccagc tctttctcga tttgcaggtg cgggatcata cagcaggtgg tgccctggcg
    19921 agcaagttgt acgaacgctt ctgaagaatt aacgatatgg cagggtacgc tgccaggagg
    19981 aagatcgaag ttttgctgca aaaaggcctg gtgcatatcg tcaagatggt caaacgcgac
    20041 cactggcgct ttcaataatg ccgaacgcgt tacgccgtta ggaaaatatt tttcggcaaa
    20101 cggttttgag ctgacgaaca gatagtcgag cgcaccgagt ttatcgacaa gacaactcgg
    20161 cagcgcctga tgttgaatac tcaccgcgcc gaccacttcg ccgcggcgca gacgttcctg
    20221 agtacgggtt tcatcttcta cctgcaagtt gaggcggata ggcgaatcag ccaacacagg
    20281 agccagtgcc ggaagcaacc acgtcgccag actgtcggcg ttgaccgcca gtgaaagcag
    20341 cagcggagtc gaaccggttt gttcatcgcc cagccactct tcttccagca actccacctg
    20401 gcgcagcagt gccagcagtt tttgcccttg ttccgtcggg cgcggcggta cggtacgcac
    20461 caacagcggc tgcccgaaca tattttccag ttgcttaatg cgctgtgaga cggctgattg
    20521 tgtaatgcac agcttttgtg ccgcgcgctc aaatcctcgt tcacgtatca ccgcatccag
    20581 tgcctgtaat gttctgtagt ccgggcgttt cattgctgtt gtctgctcct ggtaaatgct
    20641 ttttcctgca ctatgacaca attttgtgtc aggttgcata taccgaagcc gtcggcgggt
    20701 aacatttgta tcaatggtta aacgcgcagc ttaaaagatc agggatttgc gctattgcat
    20761 ttcgcttagc gacttcgcgc cctggttatg ttgaatggcg tggcgttatt gcctctgttc
    20821 gcaccgtgga gcggcatacc cagaccgttg tatgctttat ttgcggggcg aaaggggatg
    20881 cccgccattg cgcgatattt tatggatgag ttaaccaccc agcttgccaa cggggtctga
    20941 atcgcttttt ttgtatataa tgcgtgtgaa atttcatacc acaggcgaaa cgatcatgac
    21001 gcaggatgaa ttgaaaaaag cagtaggatg ggcggcactt cagtatgttc agcccggtac
    21061 cattgttggt gtaggtacag gttccaccgc cgcacacttt attgacgcgc tcggcacgat
    21121 gaaaggccag attgaagggg ccgtttccag ttcagatgct tccactgaaa aactgaaaag
    21181 cctcggcatt cacgtttttg atctcaacga agtcgacagc cttggcatct acgttgatgg
    21241 cgcagatgaa atcaacggcc acatgcaaat gatcaaaggc ggcggcgcgg cgctgacccg
    21301 tgaaaaaatc attgcttcgg ttgcggaaaa atttatctgt attgccgacg cctccaagca
    21361 ggttgatatt ctgggtaaat tcccgctgcc agtagaagtt atcccgatgg cacgtagtgc
    21421 agtggcgcgc cagctggtga aactgggcgg tcgtccggaa taccgtcagg gcgtggtgac
    21481 cgataatggc aacgtgatcc tcgacgtcca cggcatggaa atccttgacc cgatagcgat
    21541 ggaaaacgcc ataaatgcga ttcctggcgt ggtgactgtt ggcttgtttg ctaaccgtgg
    21601 cgcggacgtt gcgctgattg gcacacctga cggtgtcaaa accattgtga aatgatctga
    21661 cgggggaacc tcccccgtta aaaaaattct cttcattaaa tttggtgaca tgtgtcacgc
    21721 ttttaccagg caattgtcga ttgctctaaa taaatcctct aaaccagcat attcatccaa
    21781 gaattacctt tgcgtgatat ttcctcaaca ttgcgacgca aacgttcata ttgccgcaat
    21841 attatttttt gatatgttga aaggcggatg caaatccgca cacaacattt caaaagacag
    21901 gattgggtaa atggcaaagg tatcgctgga gaaagacaag attaagtttc tgctggtaga
    21961 aggcgtgcac caaaaggcgc tggaaagcct tcgtgcagct ggttacacca acatcgaatt
    22021 tcacaaaggc gcgctggatg atgaacaatt aaaagaatcc atccgcgatg cccacttcat
    22081 cggcctgcga tcccgtaccc atctgactga agacgtgatc aacgccgcag aaaaactggt
    22141 cgctattggc tgtttctgta tcggaacaaa ccaggttgat ctggatgcgg cggcaaagcg
    22201 cgggatcccg gtatttaacg caccgttctc aaatacgcgc tctgttgcgg agctggtgat
    22261 tggcgaactg ctgctgctat tgcgcggcgt gccggaagcc aatgctaaag cgcaccgtgg
    22321 cgtgtggaac aaactggcgg cgggttcttt tgaagcgcgc ggcaaaaagc tgggtatcat
    22381 cggctacggt catattggta cgcaattggg cattctggct gaatcgctgg gaatgtatgt
    22441 ttacttttat gatattgaaa acaaactgcc gctgggcaac gccactcagg tacagcatct
    22501 ttctgacctg ctgaatatga gcgatgtggt gagtctgcat gtaccagaga atccgtccac
    22561 caaaaatatg atgggcgcga aagagatttc gctaatgaag cccggctcgc tgctgattaa
    22621 tgcctcgcgc ggtactgtgg tggatattcc ggcgctgtgt gatgcgctgg cgagcaaaca
    22681 tctggcgggg gcggcaatcg acgtattccc gacggaaccg gcgaccaata gcgatccatt
    22741 tacctctcca ctgtgtgaat tcgacaacgt ccttctgacg ccacacattg gcggttcgac
    22801 tcaggaagcg caggagaata tcggcttgga agttgcgggt aaattgatca agtattctga
    22861 caatggctca acgctctctg cggtgaactt cccggaagtc tcgctgccac tgcatggtgg
    22921 tcgtcgtctg atgcacatcc acgaaaaccg tccgggcgtg ctaactgcgc tgaacaaaat
    22981 cttcgccgag cagggcgtca acatcgccgc gcaatatcta caaacttccg cccagatggg
    23041 ttatgtagtt attgatattg aagctgacga agacgttgcc gaaaaagcgc tgcaggcaat
    23101 gaaagctatt ccgggtacca ttcgcgcccg tctgctgtac taattcccct tctctgaaaa
    23161 ttaacgggca ggtcactgac ttgcccgttt ttttatccct tctccacacc gttgccgcgt
    23221 tgcgcattct actttgcgag tctgctccag aaaataaatc aaagtcagat caatgcgttg
    23281 tgtttattta atggggattt acgatggcgg gacagcatgg ggctgtaacg gtaaagccct
    23341 caccgaagcg agggcttaaa ggagaagggt tatgatgcga cttgtcatca tactgattgt
    23401 actgttactg ataagtttcg gagcttatta acagtcagtc tcaagggagg agtcatcctc
    23461 ccttaccctt actcactaaa ttaggtcaaa gaatcaacga tgtcaatcag ggcgatgcgg
    23521 ggtgcatcgc cctgaaccga actttttgat agccacttca tcgcccacgt ttactgccgt
    23581 aatagatcct ttttcccaaa aattatctct gttttgcatg gggtttttcg agcacgctcg
    23641 aagaaaagat ttaaaggtag atcaatgcgt tgtggttatt taaagagagc ttacgatagc
    23701 tgggcagcat ggggctgtaa cggtaaagcc ctcaccgaag cgagggcttg aaggagaagg
    23761 gttatgatgc gacttgccat catactgatt gtactgttac tcataagttt cccagcttat
    23821 taacagtcag tcacagggga ggagcaatcc tcccttaccc ttactcacta aattaggtta
    23881 aagaatcaac gatgtcaatc agggcgatgc aggtgtatcg cccttaccac tcccagactt
    23941 tcgacggtgt aaccaccgca ggaagaggga tatcccactc ttcaacgggg agtttttcca
    24001 ccaactgaca atcatgcgca taacccaccg gttgcgtttt atagtgctgc cagttttgta
    24061 aggtccgatc ataaaaacca ccgcccattc ccaggcgctg accgtactca tcaaaggcga
    24121 ccagcggtgt gatcagcacg tctaatcggg aaaggggtag cacgtcacgc acatccagtt
    24181 ttggctcatg gatcttcaac ctgttcatca ccagttcgct ttgcggatgg taattcagga
    24241 acagcaaatt accggcacta aagggatgca aaactggaag atatacgcgc ttaccggcgc
    24301 gccagagttg ttctattagc ggctgggtgt cgagttcacc atcaaaagag aggaatacag
    24361 cgaccgtatg cgccatcacc accgggggat aagtcatcat ccgggtagcg gcttgttgac
    24421 ccatttccag ctgttgttcc ggcgttaacg cacgacgacg ttgccgaatc attttgcgga
    24481 tttcttgtcg ggataatgtc agtgggagct ctggtagttg cgtcatggct ggtgccagat
    24541 aagaagggaa tctccgagat gccgccgcag gctgtaaccc ttgaaccctt ggttcaaggt
    24601 gaatgtgtcg tcgcagtttt aaggcttctc ggacgaaccg agcatgctca ccaaccgcgg
    24661 agcgccacat tcttgtggta tgaaatatcg gctcagggga ctggcccgct tgcgaacatc
    24721 tcagagaaat tttgtcttca cggttactct accacagtaa accgaaaagt gtcattcaaa
    24781 gttttggtta gttttttcgg tgatgcgacc ttgttcaagt aacgcttgtt ctatggtctg
    24841 ctgcagcatc cgaatacgct gttccatact tgccgcgtag tcacgagtct ttgctttttc
    24901 ttgcgctaac tcatagctga tattcaatgc ggcaatgaag accaactgtt cagtatttgt
    24961 gactctagtg cgttctttca gatcttgcaa ccgttggttc agatcgtccg ctgcctgatt
    25021 caacgcatcc ctttggtcag gcgggcagtt cacacgcagt gaacggccaa aaatttggat
    25081 atcgacgggt tgtgcagaca tgccaccttc ctgctgattg actgcgctgc cttcgtcttt
    25141 agacccatgg tctgcgaagg ggcgacacta tagctaccct gatgagaaga gacaagccct
    25201 tttctggtcc accagggccc aaagtggtag catatcatga atattcctcc ctttgacgac
    25261 gaatgcttat gtctatacag aacgaaatgc ctggttacaa cgaaatgaac cagtatctga
    25321 accaacaagg gacgggtctg acccctgccg agatgcatgg tttaatcagt gggatgatat
    25381 gtggcggtaa cgatgacagc tcatggctgc cgctacttca cgacctgacg aacgaaggca
    25441 tggctttcgg tcatgagctg gcacaggcac tgcgtaaaat gcactctgcc accagcgatg
    25501 ccctgcagga tgacggcttc ctttttcagc tttatctgcc tgatggcgat gatgtcagcg
    25561 ttttcgatcg ggctgatgca ctggcaggtt gggtcaatca cttcctgctt ggtcttggcg
    25621 ttacgcaacc gaagctggac aaagtgaccg gcgaaaccgg tgaagccatc gacgatctgc
    25681 gtaacatcgc gcagctgggc tacgacgaag acgaagatca ggaagagctt gaaatgtcgc
    25741 ttgaagagat catcgaatac gttcgtgttg ccgcgctgtt atgccacgac acctttactc
    25801 atccgcaacc gaccgcgcca gaagtacaaa aaccgactct acactaaaaa caaaaaacgt
    25861 aaggagagtg ttatgagtga gatatcccgg caagagtttc agcgtcgccg tcaggccctg
    25921 gtggagcaaa tgcaacccgg cagcgccgcg ctgatttttg ctgcaccaga agtaacacgt
    25981 agcgccgaca gcgaataccc ctatcgtcag aacagtgact tctggtactt caccggcttt
    26041 aacgaaccgg aagcggtgct ggtgctgatt aaaagcgatg acactcataa ccacagcgtt
    26101 ctgtttaacc gcgttcgcga cctgacggcg gagatctggt ttggccgtcg cttaggccag
    26161 gatgccgcgc cagagaaact gggcgttgac cgcgcactgg cattcagcga aatcaatcag
    26221 caactttatc aactacttaa cggcctggat gtggtttacc atgcccaggg cgaatatgca
    26281 tatgctgatg aaatcgtgaa cagtgcgctg gaaaaactgc gtaaaggttc gcggcaaaat
    26341 ctcaccgcac cggcaacgat gatcgactgg cgtcctgttg ttcatgaaat gcgcctgttc
    26401 aaatcgccag aagagattgc cgtactccgc cgcgcgggag aaatcaccgc catggcacat
    26461 acacgggcga tggaaaaatg ccgtccggga atgttcgagt accatctgga aggcgaaatt
    26521 caccacgaat ttaaccgcca cggtgcgcgc tatccttcct ataacaccat tgtaggcagc
    26581 ggtgaaaacg gctgcattct gcactacacc gaaaacgaga gtgaactgcg cgacggtgac
    26641 ctggtgttga ttgacgcggg ttgtgaatac aaaggttacg cgggcgatat tacccgcacc
    26701 ttcccggtca acggcaaatt cacccaggcc cagcgtgaaa tctacgacat tgtgctggag
    26761 tctctcgaaa ccagcctgcg cctgtatcgt ccgggaactt ccattctgga agtcactggt
    26821 gaagtggtgc gcatcatggt tagcggcctg gtaaaactcg gcatcctgaa aggtaatgtt
    26881 gatgaactga tcgctcagaa cgcccatcgt cctttcttta tgcatggcct tagccactgg
    26941 ttaggactgg atgtccatga cgttggcgtt tatggtcagg atcgctcgcg cattctggaa
    27001 ccggggatgg tactgaccgt agagcctggt ctgtatatcg cgccggatgc ggatgtgcca
    27061 gaacaatatc gcggtatcgg cattcgcatt gaagacgaca ttgtgattac cgaaaccggc
    27121 aacgaaaacc tcaccgccag cgtggtgaaa aagccggaag aaatcgaagc gttgatggct
    27181 gctgcgagaa agcaatgagc gtaatcatcg tcggtggcgg catggcgggc gcgacgctgg
    27241 cgctggctat ttcccggtta agtcacgggg cgctgccggt acatttgatt gaagcgactg
    27301 cgccagagtc acatgctcat ccgggctttg atggacgagc gatagcgctg gcggcgggta
    27361 cctgtcagca actggcgcgc atcggcgtct ggcaatctct ggctgattgc gcaaccgcca
    27421 tcactaccgt gcatgtcagc gatcgtggtc atgctggatt cgtcaccctc gccgcagaag
    27481 attaccaact ggcggcgctg gggcaggttg tcgaattgca taatgtcggg caacggctgt
    27541 ttgcattgct gcgaaaagca cctggcgtaa tgctgcattg ccccgatcgc gtagctaacg
    27601 ttgcccgtac tcagagtcat gttgaagtga cgctggagag cggcgagatg ctgacgggcc
    27661 gcgtgctgat agccgctgat ggcacccatt cagcgttagc caccgcgtgc ggcgttgatt
    27721 ggcagcagga gccttacgaa caactggcag tgattgccaa cgttgctact tctgttgcgc
    27781 acgaaggccg cgcttttgaa cgcttcacgc aacatggccc gctggcgatg ttgccgatgt
    27841 ctgacggacg ctgttcgctg gtctggtgtc atccactgga acggcgcgaa gaggtgctgt
    27901 cgtggagcga cgagaagttt tgccgtgaac tccagtctgc ctttggctgg cgactggggc
    27961 aaattaccca cgccggtaaa cgcagtgctt atccgctggc attaacccgc gccgccagac
    28021 cgattaccca tcgcactgtg ctggtgggca atgcggcgca aactctgcac ccgattgccg
    28081 ggcaagggtt taacctcggt atgcgagatg tcatgagcct tgcggaaacc ctgactcagg
    28141 cgcatgagcg cggagaagac atcggtgatt acggcatatt gtgccgctat cagcagcgtc
    28201 gacagagcga tcgcgaagca accatcggcg tcacggacag cctggtacat ctttttgcca
    28261 accgttgggc accgctggtt gtcgggcgca acatcggact gatgacgatg gaattattca
    28321 ccccggcacg cgatgtgctg gcgcagcgca ccctcggttg ggtggcgcgt tgaaggcggt
    28381 tttaaggaat aaacaatgca aagtgttgat gtagccattg ttggcggcgg catggtgggg
    28441 ctggcggttg cctgcggctt acaggggagc ggcttacgcg ttgccgtact ggagcagcgc
    28501 gtaccggaac ctctggcggt agatgcacta ccacaactgc gcgtttcggc tatcaatgcc
    28561 gccagcgaaa aattactcac ccgtcttggc gtctggcagg acattctcgc tcgtagggcc
    28621 agttgttatc acggcatgga agtgtgggac aaagacagtt ttggtcacat ctcgtttgac
    28681 gatcaaagca tgggctatag ccatcttggg cacatcgttg aaaactcagt gattcactac
    28741 gcgctgtgga acaaagcgca gcagtcgtca gatattactc tgttggcccc cacagaatta
    28801 cagcaggtcg cctggggaga aaatgaaacc ttcctgacgc tgaaagatgg cagcatgtta
    28861 acggcgcgtc tggtgattgg cgcggacggt gctaattcct ggttgcgcaa caaagcagat
    28921 attccgctga ctttctggga ttatcagcat cacgcgctgg tagcgaccat tcgcacggaa
    28981 gaaccgcatg atgcggtggc gcggcaggtt ttccatggcg aaggcattct ggccttttta
    29041 ccgcttagcg atccgcatct ttgctcgatt gtctggtcac tgtcgccaga ggaagcgcag
    29101 cggatgcagc aggcaagtga agacgaattt aatcgcgcgt taaatatcgc ttttgataat
    29161 cgccttggct tatgcaaggt tgagagcgcg cgtcaggtgt tcccgctgac ggggcgttat
    29221 gcgcgccagt ttgccgcaca ccgtctggcg ctggtgggcg acgccgcgca taccattcac
    29281 ccactggcgg ggcagggcgt taatctcgga tttatggacg ccgcagagct gattgctgaa
    29341 ctgaaacggt tgcatcgtca gggtaaagac atcgggcagt acatttacct gcgccgctat
    29401 gaacgtagcc gcaagcacag cgcggcgctg atgctggctg gtatgcaggg ttttcgcgat
    29461 ctcttttccg gcgctaatcc ggcgaaaaaa ctgctgcgtg atattggttt gaaactggcc
    29521 gacacgcttc ctggcgttaa gccgcaactt atccgccagg caatgggatt aaacgatttg
    29581 cctgaatggc tgcgttaaaa atttctcctc tgttgtttat ttgataccca tcacactttc
    29641 atctcccggt tttttcgccg ggagattttc ctcatttgaa ataaactaat ttcacctccg
    29701 ttttcgcatt atattttcta atgccatgat tttttgattt agtgtttttt gacatttttt
    29761 tagctcttaa tattgtctta ttcaaattga ctttctcatc acatcatctt tgtatagaaa
    29821 ttggtgtatt ttttggtttt ttattctgtc gcgatttttg cattttttaa ccataagcta
    29881 atgtgatgat caattttacc ttatggttaa cagtctgttt cggtggtaag ttcaggcaaa
    29941 agagaacgat tgcgttgggg accgggagtg gctccgatgc tgggttttgt ggtgataatt
    30001 tcaccatgaa aaagttgtca gccccgctta ttcaatgagg acaagatggc acaacagact
    30061 cctttgtacg aacaacacac gctttgcggc gctcgcatgg tggatttcca cggctggatg
    30121 atgccgctgc attacggttc gcaaatcgac gaacatcatg cggtacgtac cgatgccgga
    30181 atgtttgatg tgtcgcatat gaccatcgtc gatctccgcg gcagccgcac ccgggagttt
    30241 ctgcgttatc tgctggcgaa cgatgtggcg aagctcacca aaagaggcaa agccctttac
    30301 tcggggatgt tgaatgcctc tggcggtgtg atagatgatc tcatcgtcta ctactttact
    30361 gaagatttct tccgcctcgt tgttaactcc gccacccgcg aaaaagacct ctcctggatt
    30421 acccaacacg ctgaaccttt cggcatcgaa attaccgttc gtgatgacct ttccatgatt
    30481 gccgtgcaag ggccgaatgc tcaggcaaaa gctgccacac tgtttaatga tgctcagcgt
    30541 caggcggtgg aagggatgaa accgttcttt ggcgtgcagg tgggcgatct gtttattgcc
    30601 accactggtt ataccggtga agcgggctat gaaattgcgc tgcccaatga aaaagcggcc
    30661 gatttctggc gtgcgctggt ggaagcgggc gttaagccct gcggtctggg ggcgcgtgac
    30721 acgctgcgtc tggaagcggg catgaatctt tatggtcagg agatggacga aactatttct
    30781 cctttagccg ccaacatggg ctggactatc gcctgggaac cgacagatcg tgactttatc
    30841 ggtcgtgaag ccctggaagt gcagcgtgag catggtacag aaaaactggt tggtctggtg
    30901 atgaccgaaa aaggcgtgct gcgtaatgaa ctgccggtac gctttaccga tgcgcagggc
    30961 aaccagcatg aaggcattat caccagcggt actttctccc cgacgctggg ttacagcatt
    31021 gcgctggcgc gcgtgccgga aggtattggc gaaacggcga ttgtgcaaat tcgcaaccgt
    31081 gaaatgccgg ttaaagtgac aaaacctgtt tttgtgcgta acggcaaagc cgtcgcgtga
    31141 tttacttttt tggagattga ttgatgagca acgtaccagc agaactgaaa tacagcaaag
    31201 aacacgaatg gctgcgtaaa gaagccgacg gcacttacac cgttggtatt accgaacatg
    31261 ctcaggagct gttaggcgat atggtgtttg ttgacctgcc ggaagtgggc gcaacggtta
    31321 gcgcgggcga tgactgcgcg gttgccgaat cggtaaaagc ggcgtcagac atttatgcgc
    31381 cagtaagcgg tgaaatcgtg gcggtaaacg acgcactgag cgattccccg gaactggtga
    31441 acagcgaacc gtatgcaggc ggctggatct ttaaaatcaa agccagcgat gaaagcgaac
    31501 tggaatcact gctggatgcg accgcatacg aagcattgtt agaagatgag taacggcttt
    31561 attcctcttc tgcgggagag gatcagggtg aggaaaattt atgcctcacc ctcactctct
    31621 tcgtaaggag agaggttcac aattcactgc acgtttcagg aaccatcgct catgacacag
    31681 acgttaagcc agcttgaaaa cagcggcgct tttattgaac gccatatcgg accggacgcc
    31741 gcgcaacagc aagaaatgct gaatgccgtt ggtgcacaat cgttaaacgc gctgaccggc
    31801 cagattgtgc cgaaagatat tcagcttgcg acaccaccgc aggttggcgc accggcgacc
    31861 gaatacgccg cactggcaga actcaaggct attgccagtc gcaataaacg cttcacgtct
    31921 tacatcggca tgggttacac cgccgtgcag ctaccgccgg ttatcctgcg taacatgctg
    31981 gaaaatccgg gctggtatac cgcgtacact ccgtatcagc ctgaagtctc ccagggccgc
    32041 cttgaagcac tgctcaactt ccagcaggta acgctggatt tgactggact ggatatggcc
    32101 tctgcttctc ttctggacga ggccaccgct gccgccgaag caatggcgat ggcgaaacgc
    32161 gtcagcaaac tgaaaaatgc caaccgcttc ttcgtggctt ccaatgtgca tccgcaaacg
    32221 ctggatgtgg tccgtactcg tgctgaaacc tttggttttg aagtgattgt cgatgacgcg
    32281 caaaaagtgc tcgaccatca ggacgtcttc ggcgtgctgt tacagcaggt aggcactacc
    32341 ggtgaaattc acgactacac tgcgcttatt agcgaactga aatcacgcaa aattgtggtc
    32401 agcgttgccg ccgatattat ggcgctggtg ctgttaactg cgccgggtaa acagggcgcg
    32461 gatattgttt ttggttcggc gcaacgcttc ggcgtgccga tgggctacgg tggcccacac
    32521 gcggcattct ttgcggcgaa agatgaatac aaacgctcaa tgccgggccg tattatcggt
    32581 gtatcgaaag atgcagctgg caataccgcg ctgcgcatgg cgatgcagac tcgcgagcaa
    32641 catattcgtc gtgagaaagc gaactccaac atttgtactt cccaggtact gctggcaaac
    32701 atcgccagcc tgtatgccgt ttatcacggc ccggttggcc tgaaacgtat cgctaaccgc
    32761 attcaccgtc tgaccgatat tctggcggcg ggcctgcaac aaaaaggtct gaaactgcgc
    32821 catgcgcact atttcgacac cttgtgtgtg gaagtggccg acaaagcggg cgtactgacg
    32881 cgtgccgaag cggctgaaat caacctgcgt agcgatatcc tgaacgccgt ggggatcacc
    32941 cttgatgaaa ccaccacgcg tgaaaacgta atgcagcttt tcagtgtgct gctgggcgat
    33001 aaccacgggc tggagatcga cacgctggac aaagacgtgg ctcacgacag ccgctctatc
    33061 cagcctgcga tgctgcgcga cgacgaaatc ctcacccatc cggtgtttaa tcgctatcac
    33121 agcgaaaccg aaatgatgcg ctatatgcac tcgctggagc gtaaagatct ggcgctgaat
    33181 caggcgatga tcccgctggg ttcctgcacc atgaaactga acgccgccgc cgagatgatc
    33241 ccaatcactt ggacggaatt tgccgaactg cacccgtttt gcccgccgga acaggctgaa
    33301 ggttatcagc agatgattgc gcagctggct gactggctgg tgaaactgac cggttacgac
    33361 gccgtttgta tgcagccgaa ctccggcgca cagggcgaat acgcgggcct gctggcgatt
    33421 cgtcattatc atgaaagccg caacgaaggg catcgcgata tctgcctgat cccggcttct
    33481 gcgcacggaa ctaaccccgc ttctgcacat atggcaggaa tgcaggtggt ggttgtggcg
    33541 tgtgataaaa acggcaacat cgatctgact gatctgcgcg cgaaagcgga acaggcgggc
    33601 gataacctct cctgtatcat ggtgacttat ccttctaccc acggcgtgta tgaagaaacg
    33661 atccgtgaag tgtgtgaagt cgtgcatcag ttcggcggtc aggtttacct tgatggcgcg
    33721 aacatgaacg cccaggttgg catcacctcg ccgggcttta ttggcgcgga cgtttcgcac
    33781 ctcaacctgc ataaaacttt ctgcattccg cacggcggtg gtggtccggg tatgggaccg
    33841 atcggtgtga aagcgcatct ggcaccgttt gtaccgggtc atagcgtggt gcaaatcgaa
    33901 ggcatgttaa cccgtcaggg cgcagtttct gcggcaccgt tcggtagcgc ctctatcctg
    33961 ccaatcagct ggatgtacat ccgcatgatg ggcgcagaag ggctgaaaaa agcaagccag
    34021 gtggcaatcc tcaacgccaa ctatattgcc agccgcctgc aggatgcctt cccggtgctg
    34081 tataccggtc gcgacggtcg cgtggcgcac gaatgtattc tcgatattcg cccgctgaaa
    34141 gaagaaaccg gcatcagcga gctggatatt gccaagcgcc tgatcgacta cggtttccac
    34201 gcgccgacga tgtcgttccc ggtggcgggt acgctgatgg ttgaaccgac tgaatctgaa
    34261 agcaaagtgg aactggatcg ctttatcgac gcgatgctgg ctatccgcgc agaaattgac
    34321 caggtgaaag ccggtgtctg gccgctggaa gataacccgc tggtgaacgc gccgcacatt
    34381 cagagcgaac tggtcgccga gtgggcgcat ccgtacagcc gtgaagttgc ggtattcccg
    34441 gcaggcgtgg cagacaaata ctggccgacg gtgaaacgtc tggatgatgt ttacggcgac
    34501 cgtaacctgt tctgctcctg cgtaccgatt agcgaatacc agtaattcac tgattcgagt
    34561 attttctaaa ggcgcttcgg cgccttttta gtcagatgac aaagtacaaa agtgctcaga
    34621 cagtcccctc gcccctttgg ggagagggtt agggtgaggg gaacaggccg gcactggcgc
    34681 gaatatttac cctcaccccg accctctccc tgaaagggcg agggggaaaa gcgtgccaac
    34741 attgaagatt gagccagttt gttagcaatc tcaaagatac gtcaacgaat taatttttct
    34801 cggaaaaaca aatggctata gcacttgtga ccggtggcag tcgcggcatc gggcgggcaa
    34861 ctgcattact gttggcgcaa gaagggtata cggtggcggt taattatcag caaaacctcc
    34921 acgcggcgca ggaagtgatg aacttaataa tgcaagccgg tggcaaagca ttcgtgctcc
    34981 aggcggatat cagcgacgaa aaccaggtcg ttgcgatgtt tacagcaatc gatcagcacg
    35041 atgaaccgct agcagcgctg gtcaataacg ccgggatctt gtttacccag tgcaccgttg
    35101 aaaaccttac cgcagagcga atcaaccgag tactttccac caacgtgacg ggatattttc
    35161 tctgctgccg cgaggcggta aaacgcatgg cgcttaaaaa tggtggcagt ggcggcgcta
    35221 tcgtcaatgt ctcttcggtg gcctcacggt tgggttcgcc aggggaatat gttgattacg
    35281 cggcatcgaa aggggcgatt gattcgttaa ccaccggact atcgctggaa gtcgccgcgc
    35341 aggggatccg cgttaactgc gtgcggccag ggtttattta taccgaaatg cacgccagcg
    35401 cggcgagcct ggacgcgtcg atcgcgttaa gtcgaacatc cccatgcagc gtggtggaca
    35461 ggcagaagag gtcgcgcagg ccattgtctg gctactaagt gataaagcct cttacgtcac
    35521 gggaagtttt atcgatttgg cgggcgggaa ataaaacagg gaagttgtct gaccggatgc
    35581 aacaagtatt gcatccggta cttcatcgac ttaaagcttc tcgccgttgc tggcaatcac
    35641 ctctttgtac cagttaaagc tcttcttacg tgaacgcgac atatcgccag taccgtcgtc
    35701 atgtttattc acatagataa agccgtagcg tttgctgtac tgcccggtag tgaatgacac
    35761 gcagtcgatg cagccccacg gtgtgtagcc catcagatcc acaccatcgt aagtcactgc
    35821 ttttttcatc tcttcaatat gggcgcgcag gtagtcaatg cggtagtcgt cgttgatgct
    35881 gccatcttct tccactttgt cgtaagcgcc aaaaccgttt tcgacaataa acagcggcct
    35941 ctgataacgc tcatacagtt cgcaaagggc atagcgcaga cctactggat caatctgcca
    36001 gccccagtca gacgctttaa catacgggtt cggtacgctg ccttcaaaac cagagatcgc
    36061 atcgccggtg ccgccttcgg ccttcactgc gttggtcatg taatagctga aaccaagata
    36121 atcgcaggtg ccttcgcgca gtacatcgag atcgccgtct tccattttga tattaaatcc
    36181 gcgacgttcc cactcgttca atacatagga cgggtaatag ccgcatagct gcacatcggt
    36241 aaagacgtag cgttcgcgca tcgactcctg agcgaacatc acatcgtccg ggttacatga
    36301 gtagggatag agcggcacca tcgccagcat acagccgact ttcatctccg ggttaatgcg
    36361 acgcgcggct ttcactgcca gggcgctggc gacaaactgg tgatgcagca cctggtacat
    36421 cgtctcttcc gggttgtcat gctcggtata caccacgccg gagcagcagt aaccaaacag
    36481 cggcgcacgc cagttacgct ggttgtttat ttcgttgaag gtcatccagt atttgacttt
    36541 atgcttgtag cgttcgaata ccacttccgc gaaacggaca aagaaatcaa cgaatttacg
    36601 gttggtccag ctaccgtatt gctgcaccag atgcagcggc atttcaaagt gggagagggt
    36661 tatcaccggt tcgatgttgt atttcagcag ttcatcgaac agagagtcgt aaaacttcag
    36721 cccttcttcg tttggctgag cttcatcacc ttttggaaaa atacgcgtcc aggcgatgga
    36781 agtccgaaaa catttgaagc ccatttcggc aaatagcttg atgtcttcct tgtagtgacc
    36841 ataaaaatca acggcttcat ggtttggata gtattttcct ggcaagactt ctttggtgat
    36901 ttcgcgcggc acgccgtgtg cgccaccggt cagaacgtca caaatgctcg gtcctttgcc
    36961 gcctttgttc cagccgcctt cgacctgatg agcggcaacg gcgccacccc ataagaaatc
    37021 tttcggtaag gtgagttttt tcactatcat aggctccaga tcagtcaatt cttacaggaa
    37081 atagtaccat gcagccaaac gacatcactt ttttcaacgt ttccaggatg acattctggc
    37141 tgggcgtaaa accatcacca tccgcgacga gtctgaatcg cacttcaaaa cgggtgatgt
    37201 gcttcgtgtc ggacgttttg aagatgacgg ttatttttgc acgattgaag tcaccgcaac
    37261 ctcaaccgta acgctggata cactgacgga aaaacatgca gagcaggaaa atatgaccct
    37321 gcctgaactg aaaaaggtca ttgctgacat ctatcccggt cagacacaat tttatatgat
    37381 tgaatttaaa tgtctttaaa ttgaactcac gcctgttagt tgaaatttcg catttaattt
    37441 catgattttt aggagattag tctcaaatca gaatttattt tagctaacag gtgttcactg
    37501 gaactattct cagttacgct ggagaggtat acatgtgcgt gtacgtttct ctggagtaag
    37561 ttatggttca gaagcccctc attaagcagg gatattcgct ggcagaggaa attgccaaca
    37621 gcgtcagtca cggcattggg ctggtgtttg gtatcgttgg gctggtgttg ctactggttc
    37681 aggcggtgga tctcaatgcc agcgccacag cgataaccag ttacagcctc tatggcggca
    37741 gtatgatcct gctgttcctc gcttcgacgc tctatcacgc cattcctcat caacgggcaa
    37801 aaatgtggct gaagaaattt gaccattgcg ctatttacct gttgattgcc ggaacctaca
    37861 cgccgttttt gctggtgggg ctggattctc cgttagcgcg cgggttgatg attgttatct
    37921 ggagcctagc attgctgggg attctgttca aactgaccat cgcgcaccga ttcaaaattt
    37981 tatctctggt gacttatctg gcgatgggct ggctgtcgct ggtggtaatt tatgaaatgg
    38041 cagttaagct cgcggcgggc agcgttacct tactggcggt aggcggcgtg gtttattcgc
    38101 tcggggtgat tttctacgtc tgcaaacgca ttccatacaa ccatgccatc tggcacggct
    38161 tcgtgctcgg cggtagtgtg tgccactttc tggcgatcta tttgtatatt gggcaggcgt
    38221 aatgggtatg cctttgtagg cctgataaga cgcgtcagcg tcgcatcagg catcggagcg
    38281 caaccgccgg atgcggcgtg aacgccttat ccgatctacg ggcctcgtcc ctgttgtgat
    38341 aaaggcatca aatcccagac atgataagac gcgtcagcgt cgcatcaggc gataaatgcc
    38401 tgatgcgata cagagattta ctcttcgagc gaatacggca gcggctcgat atgcagtgta
    38461 ttcgcatcgt cgcgtacgcg gaagatgctg tccggttcca tatcgttatt catgacgacc
    38521 tgtaccacga cctgaccatc ttccagtttt accgcagcca gcaccgtacc ggtacgacgc
    38581 cagttctcgc ccattttcag ctctaagtct tcaccagctt ccggcagtcg gctgacacta
    38641 cctgccagca accagagcgc gcgtttgttg gcaccacgga attttgctcg cgccaccatc
    38701 tcttgtccgg tataacagcc tttcttaaag ctgataccgc ccagcgcctg gagattggtc
    38761 gcctgtggga taaactgccc gctgttggcg gcatcaatca ccgggaaacc agcttcaatg
    38821 tttaatgcca gccactgttg gctattgttc agttccgctt caccgcgcag tttatcggtc
    38881 agcatattag cagtagcttc atcggttacg atcaggaaac gttctgccgg gtgttcaaac
    38941 catagcaaag tggtcgcgcc ttctttgact acctgttttt ctttcgaagg cagttcgcta
    39001 aagagatttg ccagcgcggc gcgcgcctga aaaccggcaa caccaagcag cacacgctcg
    39061 tcgtctggcg cgatggtcac tttagagaat accgcatatt ttttcagttc agtcagctgc
    39121 ggttcacgca cgctgcgccg ttcaatccat gcaaagccat cgccgtcgcg gaacagacgt
    39181 aaattgctcc acattttacc tttggcgtcg caatgggcgg cgagcaggtg ctgatcttct
    39241 gccatctggc tgacatctgc tgtcacctga ccctgcatat atttttcgct gtccgcgcca
    39301 gtaatggtgg caagcgccca gtcatcaagc gtcatcagcg tcagcggtaa acgggcagaa
    39361 gccgtaggct gacgtggagg aaaaggtgta aaagccataa tagagtcctg agttgcttaa
    39421 cggcgtatta atgactaatg gtaaaagagc tatgaggcaa tgcaagtgct ttcactacgc
    39481 gatctgtgcc ttctgcgagg cgactgccaa aaaaatgaag cccttgaatt gttgtgaatt
    39541 aatttcgcga tcgcgatggt gtttactgat attcccgtaa aagaagtcga aaaacacgtt
    39601 acaatagccg ggtactggat tttcgtgaga caggaagaag atggacatta acaacaaagc
    39661 ccgaattcat tgggcatgcc gccgtggtat gcgcgaactc gatatttcaa tcatgccgtt
    39721 tttcgaacat gagtacgaca gcttaagcga tgacgaaaaa cgcatcttta ttcgtctgct
    39781 ggaatgtgac gatccggacc tgtttaactg gctgatgaat cacggtaaac cggccgatgc
    39841 agaactggaa ataatggtcc gactcatcca gacacggaac cgggaacgtg gtcctgtggc
    39901 aatctgattt gcgcgtctcc tggcgcgcac agtggctttc cttgctgatt catgggctgg
    39961 ttgccgctgt tattttactc atgccctggc cgctcagtta caccccgtta tggatggtgt
    40021 tactttcgct ggtggtattt gattgcgttc gcagccagcg gtgtattaat gctcgccagg
    40081 gggaaattcg cttgttgatg gacgggcgtt tgcgttggca agggcaggag tggagcatcg
    40141 tcaaagcacc gtggatgatt aagagcggca tgatgctgcg tttacgttct gatggcggta
    40201 aacggcaaca tttatggctg gcagccgaca gcatggacga agccgaatgg cgggatttac
    40261 ggcggatttt gttgcaacaa gagacgcaaa gataaagatg acggcaaagc atatgcgcta
    40321 agccgtcagt gagatcaggc gtaatgctct gccatttcgt tgaggatttg ctcgcaccag
    40381 ctctgaatac gctcgtcgct aaggtcatac tggttagttt catccagcgc cagacccacg
    40441 aacagttgcc cgtcagcaat caccggtttc gggctggtaa attcatatcc ttccgttggc
    40501 cagtagccga cgaacttcac gcctttggtc gagagtttgt catgcagcat accgagcgca
    40561 tcgaggaacc actcgccgta tcccagttga tcgccaagcc catacagcgc aataatttta
    40621 ccttcaaggt tcaggtcgtc gagctgatcc cagacggctt cccagtcttc ctggatttca
    40681 ccaaaatccc aggtcgggat acccagaatg agcacatcgt actgctccat taatttcggg
    40741 gagtcgtcct tgaggttatg taaggtcacc agttctgggc cgataatatc gcggattttt
    40801 tctgccgcca tttcggtgta acaggtgctg gaaccgtaaa aaagacccat attcataacg
    40861 taagcttctc gattctgctg tggcttttgc agctagtgta tcagaatcgc tttagtcacg
    40921 gcacaataag gcataatgga tcaaatgagt gaccataagg ggcgcaagtg aaacaggagc
    40981 tggcacgcat cgagcagttt cttgatgctc tgtggctgga aaaaaatctg gctgaaaata
    41041 cgttgaacgc ttaccgtcgc gatctgtcaa tgatggtgga gtggttgcat caccgcgggt
    41101 tgacgctggc gacggcgcaa agtgacgatt tgcaggcatt actggcagaa cggctggagg
    41161 gcgggtataa agccaccagc tcagcgcgtt tgctgagtgc agtgcgccga ttgttccagt
    41221 atctttatcg cgaaaagttt cgtgaagacg atcccagtgc gcatctcgct tcaccgaaat
    41281 tgccccagcg tttgccaaaa gatttaagcg aagcgcaggt cgaacgttta ttacaggcgc
    41341 cattaattga tcagccactg gagctacgcg ataaagccat gcttgaagtg ttgtatgcta
    41401 ccgggctgcg tgtctctgaa ctggtcgggc tgacaatgag tgatatcagc ctgcgtcagg
    41461 gcgtagtgcg ggtcattggt aaaggcaaca aagagcgcct ggtgccgtta ggggaagagg
    41521 cggtttactg gctggaaacc tatctggaac atgggcgtcc gtggctgctg aatggtgtgt
    41581 caattgacgt gttgtttccc agccagcgtg cgcagcagat gacgcgacag accttctggc
    41641 accgcattaa acattatgcc gtgctggcgg gtatcgacag cgaaaagctg tcaccgcatg
    41701 tgttgcgtca cgcttttgcc actcatttat taaatcatgg tgcggattta cgcgtggtgc
    41761 agatgctact gggccacagc gatctttcca ccacgcaaat ttatacgcat gtcgctaccg
    41821 agcgtctgcg gcaacttcat caacagcatc acccgcgggc gtgatgtctg aaaagaacgg
    41881 gaagatttat gaagaaaggt tttatgttgt ttactttgtt agcggcgttt tcaggctttg
    41941 ctcaggctga tgacgcggca attcaacaaa cgttagccaa aatgggcatc aaaagcagcg
    42001 atattcagcc cgcgcccgta gctggcatga agacagttct gactaacagc ggtgtgttgt
    42061 acatcaccga tgatggtaaa catatcattc aggggccaat gtatgacgtt agtggcacgg
    42121 ctccggtcaa tgtcaccaat aagatgctgt taaagcagtt gaatgcgctt gaaaaagaga
    42181 tgatcgttta taaagcgccg caggaaaaac acgtcatcac cgtgtttact gatattacct
    42241 gtggttactg ccacaaactg catgagcaaa tggcagacta taacgcgctg gggatcaccg
    42301 tgcgttatct tgctttcccg cgccaggggc tggacagcga tgcagagaaa gaaatgaaag
    42361 ctatctggtg tgcgaaagat aaaaacaaag cgtttgatga tgtgatggca ggtaaaagcg
    42421 tcgcaccagc cagctgcgac gtggatattg ccgaccatta cgcacttggc gtccagcttg
    42481 gcgttagcgg tactccggca gttgtgctga gcaatggcac acttgttccg ggttaccagc
    42541 cgccgaaaga catgaaagaa ttcctcgacg aacaccaaaa aatgaccagc ggtaaataat
    42601 tcgcgtgaaa caacagatac aacttcgtcg ccgtgaagtc gatgaaacgg cagacttgcc
    42661 cgctgaattg cctcccttgc tgcgccgttt atacgccagc cggggagtac gcagtgcgca
    42721 agaactggaa cgcagtgtta aaggtatgtt gccctggcag caactaagcg gtgtcgaaaa
    42781 ggccgttgag atcctttaca acgcttttcg cgaaggaacg cggattattg tggtcggtga
    42841 tttcgacgcc gacggcgcga ccagcacggc tctaagcgtg ctggcgatgc gctcgcttgg
    42901 ttgcagcaat atcgactacc tggtaccaaa ccgtttcgaa gacggttacg gcttaagccc
    42961 ggaagtggtc gatcaggccc atgcccgtgg cgcgcagtta attgtcacgg tggataacgg
    43021 tatttcctcc catgcgggag ttgagcacgc tcgctcgttg ggcatcccgg ttattgttac
    43081 cgatcaccat ttgccgggcg acacattacc cgcagcggaa gcgatcatta accctaactt
    43141 gcgcgactgt aatttcccgt cgaaatcact ggcaggcgtg ggtgtggcgt tttatctgat
    43201 gctggcgctg cgcacctttt tgcgcgatca gggctggttt gatgagcgcg gcatcgcaat
    43261 tcctaacctg gcagaactgc tggatctggt agcactgggg acagtagcgg acgtcgtgcc
    43321 gctggacgct aataatcgca ttctgacctg gcaggggatg agtcgcatcc gtgccggaaa
    43381 gtgccgtcca gggattaaag cgctgctgga agtggcaaat cgcgatgcac aaaaactagc
    43441 cgccagcgat ttaggttttg cgctgggacc gcgtctcaat gctgccgggc gactggatga
    43501 tatgtccgtt ggtgtggcgc tgttgctgtg cgacaacatc ggcgaagcgc gcgtgctggc
    43561 aaatgaactc gatgcgctaa accagacgcg aaaagagatc gaacaaggaa tgcaggttga
    43621 agccctgacc ctgtgcgaga aactggagcg cagccgtgac acgctacccg gcgggctggc
    43681 aatgtatcac cccgaatggc atcagggcgt tgtcggtatt ctggcttcgc gtatcaaaga
    43741 gcgttttcac cgtccggtta tcgcctttgc gccagcaggt gacggtacgc tgaaaggttc
    43801 cggtcgctcc attcaggggc tgcatatgcg tgatgcgctg gagcgattag acacactcta
    43861 ccccggcatg atgctcaagt ttggcggtca tgcgatggcg gcgggtttgt cgctggaaga
    43921 ggataaattc gaactctttc aacaacggtt tggcgaactg gttactgagt ggctggcccc
    43981 ttcgctattg caaggcgaag tggtgtcaga cggtccgtta agcccggccg aaatgaccat
    44041 ggaagtggcg cagctgctgc gcgatgctgg cccgtggggg cagatgttcc cggagccgct
    44101 gtttgacggt catttccgtc tgctgcaaca gcggctggtg ggcgaacgtc atttgaaggt
    44161 gatggtcgaa ccggtcggcg gcggtccact gctggatggt attgctttta atgtcgatac
    44221 cgctctctgg ccggataacg gcgtgcgcga agtgcaactg gcttataagc tcgatatcaa
    44281 cgagtttcgc ggcaaccgca gcctgcaaat tatcatcgac aatatctggc caatttagcg
    44341 tcatcttctc tataaaaaag agcgtggatt gggtacaatc ccgctcttat caccgcattt
    44401 tgactagctc aataaaagaa atcagaccat gtttgaaatt aatccggtaa ataatcgcat
    44461 tcaggacctc acggaacgct ccgacgttct tagggggtat ctttgactac gacgccaaga
    44521 aagagcgtct ggaagaagta aacgccgagc tggaacagcc ggatgtctgg aacgaacccg
    44581 aacgcgcaca ggcgctgggt aaagagcgtt cctccctcga agccgttgtc gacaccctcg
    44641 accaaatgaa acaggggctg gaagatgttt ctggtctgct ggaactggct gtagaagctg
    44701 acgacgaaga aacctttaac gaagccgttg ctgaactcga cgccctggaa gaaaaactgg
    44761 cgcagcttga gttccgccgt atgttctctg gcgaatatga cagcgccgac tgttacctcg
    44821 atattcaggc ggggtctggc ggtacggaag cacaggactg ggcgagcatg cttgagcgta
    44881 tgtatctgcg ctgggcagaa tcgcgtggtt tcaaaactga aatcatcgaa gagtcggaag
    44941 gtgaagtggc gggtattaaa tccgtgacga tcaaaatctc cggcgattac gcttacggct
    45001 ggctgcgtac agaaactggc gttcaccgcc tggtgcgtaa aagcccgttt gactccggcg
    45061 gtcgtcgcca cacgtcgttc agctctgcgt ttgtttatcc ggaagttgat gatgatattg
    45121 atatcgaaat caacccggcg gatctgcgca ttgacgttta tcgcgcgtcc ggcgcgggcg
    45181 gtcagcacgt taaccgtacc gaatctgcgg tgcgtattac ccacatcccg accgggatcg
    45241 tgacccagtg ccagaacgac cgttcccagc acaagaacaa agatcaggcc atgaagcaga
    45301 tgaaagcgaa gctttatgaa ctggagatgc agaagaaaaa tgccgagaaa caggcgatgg
    45361 aagataacaa atctgacatc ggctggggca gccagattcg ttcttatgtc cttgatgact
    45421 cccgcattaa agatctgcgc accggggtag aaacccgcaa cacgcaggcc gtgctggacg
    45481 gcagcctgga tcaatttatc gaagcaagtt tgaaagcagg gttatgagga accaacatgt
    45541 ctgaacaaca cgcacagggc gctgacgcgg tagtcgatct taacaatgaa ctgaaaacgc
    45601 gtcgtgagaa gctggcgaac ctgcgtgagc agggaattgc cttcccgaac gatttccgtc
    45661 gcgatcatac ctctgaccaa ttgcacgcag aattcgacgg taaagagaac gaagaactgg
    45721 aagcgctgaa catcgaagtc gccgttgctg gccgcatgat gacccgccgt attatgggta
    45781 aagcgtcttt cgttaccctg caggatgtgg gtggccgcat tcagctgtac gttgcccgtg
    45841 acgatctgcc ggaaggcgtt tacaacgagc agttcaaaaa atgggacctc ggcgacatcc
    45901 tcggcgcgaa aggtaaactg ttcaaaacca aaaccggcga actgtctatc cactgtaccg
    45961 agctgcgtct gctgaccaaa gcactgcgtc cgctgccgga taaattccac ggcttgcagg
    46021 atcaggaagc gcgctatcgt cagcgttatc tggatctcat ctctaacgat gaatcccgta
    46081 acacctttaa agtacgctcg cagatcctct ctggtattcg ccagttcatg gtgaaccgcg
    46141 gctttatgga agttgaaacg ccgatgatgc aggtgatccc aggcggtgcc gctgcgcgtc
    46201 cgtttatcac ccaccataac gcgctggatc tcgacatgta cctgcgtatc gcgccggaac
    46261 tgtacctcaa gcgtctggtg gtcggtggct tcgagcgtgt attcgaaatc aaccgtaact
    46321 tccgtaacga aggtatttcc gtacgtcata acccagagtt caccatgatg gaactctaca
    46381 tggcttacgc agattacaaa gatctgatcg agctgaccga atcgctgttc cgtactctgg
    46441 cacaggatat tctcggtaag acggaagtga cctacggcga cgtgacgctg gacttcggta
    46501 aaccgttcga aaaactgacc atgcgtgaag cgatcaagaa atatcgcccg gaaaccgaca
    46561 tggcggatct ggataacttc gactctgcga aagcgattgc tgaatctatc ggtatccacg
    46621 ttgagaagag ctggggtctg ggccgtatcg ttaccgagat cttcgaagaa gtggcagaag
    46681 cacatctgat tcagccgacc ttcattactg aatatccggc agaagtttct ccgctggcgc
    46741 gtcgtaacga cattaacccg gaaatcacag accgctttga gttcttcatc ggtggtcgtg
    46801 aaatcggtaa cggctttagc gagctgaatg acgcggaaga tcaggcgcaa cgcttcctgg
    46861 atcaggttgc cgcgaaagac gcaggtgacg acgaagcgat gttctacgat gaagattacg
    46921 ttaccgcact ggaacatggc ttaccgccga cagcaggtct ggggattggc atcgaccgta
    46981 tggtaatgct gttcaccaac agccatacca tccgcgacgt tattctgttc ccggcgatgc
    47041 gtccggtgaa ataagcatta cgttatgctc acaaccccgg caaatgtcgg ggttttttat
    47101 ttaagctggg taaatgcaga taatcgtatt ctggcttcgc gatttgtcgc ctgcatcacc
    47161 atccacggac tgaacgccca cggcgtggca tcaataccgc gtaatacatc agctaaatca
    47221 caccattgat aatccatcac ttcatcatcg ttgatctgta acgcactggt ggtgcgtgcg
    47281 gcaaataccg gacacacttc attttccaca atgcctctcg gatcggtggc gcggtagcga
    47341 aagtcaggat agatagattc aggaggcgta atttccacgc caagctcata acggcaacgg
    47401 cggatcaccg cgtcttcgct gctttctccc agttgtgggt gcccacaaac cgagttagtc
    47461 cacacgccag gccacgcttt tttgctcagt gcgcggcggg taactaataa ttgccccttg
    47521 gcattaaaca gccaactgga gaacgcgaga tgtaagaggg tgtctgccgt gtgtgcggca
    47581 tacttttcca gcgtacccgt gggaactccc tgtgcattca ataaaatgac gtgttccgtt
    47641 tgcataattt ctcacatgta attctgatca ctcatcatta tagataattg aagagtgaaa
    47701 tgattaacca agagagcgtt tatcgacaac cgcccgtttt gagcggatgc cgacaggtaa
    47761 aaaccattta aaaaagtagc atcatatatt tgaagcgact ttcaatggcc attgcaacac
    47821 agtcagaaat gacaggaata gagagagtaa aaagcaacaa cagactgaat atagcgcgca
    47881 ttaaaaaatt catattattc tccatgctca tttttcttca gcttgcgcat cagaagccac
    47941 atacgtactg tcctgacttc taaatcttta tcagagacgg attccttcac caggtcagca
    48001 tgatgctgat agccattaaa gaagacgttc agcacaacgg cgctaagggt tgcgagcata
    48061 atgccgctat gtagcagcgg ttgcagtacg gccggtaact tagaaaagaa atcgtgagag
    48121 agcgtcggag tcatgccaac gccgagactg atcgccacaa tatagaggtt gtaacggttg
    48181 gtggtgtagt tacagcgcga cagaattcga atccctgtcg ccagtaccat gccgaacatc
    48241 actagaccag cgccgcccag cacaaattgc ggaatggagg ctaccagcac cgccattttt
    48301 ggcaccatgc caaacaggat taatataatt cccgaagaaa tacacaccca gcggctatga
    48361 acgcgcatca cgctaaccag gccaacgttt tgagaaaatg acgtgtgggg gaagctgtta
    48421 aacgtgccgc ctatcattgt ccctacgcca tcgacacgca gcccgcaaat aatatcgtgt
    48481 gaagagagtt tacgaccgac tatttcaccc agtgccagga acatccccat cgactcgata
    48541 aacacgatga
//
LOCUS       DAJTQY010000021        48089 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.021, whole genome shotgun sequence.
ACCESSION   DAJTQY010000021 DAJTQY010000000
VERSION     DAJTQY010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 48089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48089
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.021"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            187..480
                     /locus_tag="OO349_001233"
     CDS             187..480
                     /locus_tag="OO349_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCR5866379.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPHASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            complement(521..1711)
                     /gene="nepI"
                     /locus_tag="OO349_001234"
     CDS             complement(521..1711)
                     /gene="nepI"
                     /locus_tag="OO349_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HCR5866380.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(1922..2374)
                     /locus_tag="OO349_001235"
     CDS             complement(1922..2374)
                     /locus_tag="OO349_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HCR5866381.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDDSEQNLLQWHRILQKAR
                     LSAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(2427..3761)
                     /gene="adeQ"
                     /locus_tag="OO349_001236"
                     /gene_synonym="yicO"
     CDS             complement(2427..3761)
                     /gene="adeQ"
                     /locus_tag="OO349_001236"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HCR5866382.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAIGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            3936..5702
                     /gene="adeD"
                     /locus_tag="OO349_001237"
     CDS             3936..5702
                     /gene="adeD"
                     /locus_tag="OO349_001237"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HCR5866383.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     AGEISGPIVIKGRYIAGVGAEYADTPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVINGQNALLDKLDAFRYLTLDGHCPGL
                     GGKELNAYIAAGIENCHESYQLEEGRRKLQLGMSLMIREGSAASNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLALSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSRSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVMIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(5748..7139)
                     /gene="uhpT"
                     /locus_tag="OO349_001238"
     CDS             complement(5748..7139)
                     /gene="uhpT"
                     /locus_tag="OO349_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HCR5866384.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(7277..8596)
                     /gene="uhpC"
                     /locus_tag="OO349_001239"
     CDS             complement(7277..8596)
                     /gene="uhpC"
                     /locus_tag="OO349_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HCR5866385.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIMNILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(8606..10108)
                     /gene="uhpB"
                     /locus_tag="OO349_001240"
     CDS             complement(8606..10108)
                     /gene="uhpB"
                     /locus_tag="OO349_001240"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HCR5866386.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHLVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(10108..10698)
                     /gene="uhpA"
                     /locus_tag="OO349_001241"
     CDS             complement(10108..10698)
                     /gene="uhpA"
                     /locus_tag="OO349_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HCR5866387.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(10773..11063)
                     /gene="ilvN"
                     /locus_tag="OO349_001242"
     CDS             complement(10773..11063)
                     /gene="ilvN"
                     /locus_tag="OO349_001242"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCR5866388.1"
                     /translation="MQNTTHNNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIAKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(11067..12755)
                     /gene="ilvB"
                     /locus_tag="OO349_001243"
     CDS             complement(11067..12755)
                     /gene="ilvB"
                     /locus_tag="OO349_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HCR5866389.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPSPVWIDIPKDVQTAVFEIEAQPAVAEKAAAPAFSEESIRDAATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPRVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(12861..12959)
                     /gene="ivbL"
                     /locus_tag="OO349_001244"
     CDS             complement(12861..12959)
                     /gene="ivbL"
                     /locus_tag="OO349_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HCR5866390.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            13524..13613
                     /gene="tisB"
                     /locus_tag="OO349_001245"
     CDS             13524..13613
                     /gene="tisB"
                     /locus_tag="OO349_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HCR5866391.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            13893..15077
                     /gene="emrD"
                     /locus_tag="OO349_001246"
     CDS             13893..15077
                     /gene="emrD"
                     /locus_tag="OO349_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HCR5866392.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(15085..15582)
                     /locus_tag="OO349_001247"
     CDS             complement(15085..15582)
                     /locus_tag="OO349_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HCR5866393.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(15579..15941)
                     /locus_tag="OO349_001248"
     CDS             complement(15579..15941)
                     /locus_tag="OO349_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HCR5866394.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(15931..16278)
                     /locus_tag="OO349_001249"
     CDS             complement(15931..16278)
                     /locus_tag="OO349_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HCR5866395.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VVVIVMGLVLYAG"
     gene            16387..16800
                     /locus_tag="OO349_001250"
     CDS             16387..16800
                     /locus_tag="OO349_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5866396.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDMDIDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(16847..18289)
                     /locus_tag="OO349_001251"
                     /pseudo
     CDS             complement(16847..18289)
                     /locus_tag="OO349_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            complement(18324..20039)
                     /locus_tag="OO349_001252"
     CDS             complement(18324..20039)
                     /locus_tag="OO349_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HCR5866397.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAIGKGSFM
                     QGIEQITTVHAEKLNSVGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPLEGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWKNVKIAS
                     IGILFAMIGVYVGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWWHRHDPAVTFTPD
                     AKDSL"
     gene            20206..21099
                     /locus_tag="OO349_001253"
     CDS             20206..21099
                     /locus_tag="OO349_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR5866398.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHSNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDIPVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMSADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            complement(21096..21910)
                     /locus_tag="OO349_001254"
                     /pseudo
     CDS             complement(21096..21910)
                     /locus_tag="OO349_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(21910..23527)
                     /locus_tag="OO349_001255"
                     /pseudo
     CDS             complement(21910..23527)
                     /locus_tag="OO349_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
     gene            23822..24496
                     /locus_tag="OO349_001256"
     CDS             23822..24496
                     /locus_tag="OO349_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR5866399.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLMPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEY"
     gene            complement(24535..26196)
                     /locus_tag="OO349_001257"
     CDS             complement(24535..26196)
                     /locus_tag="OO349_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HCR5866400.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(26392..26820)
                     /gene="ibpB"
                     /locus_tag="OO349_001258"
     CDS             complement(26392..26820)
                     /gene="ibpB"
                     /locus_tag="OO349_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HCR5866401.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(26932..27345)
                     /gene="ibpA"
                     /locus_tag="OO349_001259"
     CDS             complement(26932..27345)
                     /gene="ibpA"
                     /locus_tag="OO349_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HCR5866402.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(27339..27411)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            27650..27982
                     /locus_tag="OO349_001260"
     CDS             27650..27982
                     /locus_tag="OO349_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HCR5866403.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(27984..29204)
                     /locus_tag="OO349_001261"
     CDS             complement(27984..29204)
                     /locus_tag="OO349_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HCR5866404.1"
                     /translation="MKQITFAPRNHLLTNTNTNTWTPDSQWLVFDVRPSGASFTGETI
                     ERVNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHRRGVI
                     AEGGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERGSFTYNDHVMHQLDPALDLR
                     NVGVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHAL
                     AFIGDTLSPKGEKVPELFIVELPQDEASWKSAGDAPLSGTETTLPAPPRGVVQRRLTF
                     THHRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRKLTHNKTD
                     IQSAFNWHPSGEWLGFVLDNRIVCAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLA
                     WMEGGQLWITETDR"
     gene            29305..30405
                     /gene="cbrA"
                     /locus_tag="OO349_001262"
     CDS             29305..30405
                     /gene="cbrA"
                     /locus_tag="OO349_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance protein"
                     /protein_id="HCR5866405.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSAEILRAVFLKQPEKSNAAYWRATHKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKDYPTRFTSPTSRM"
     gene            complement(30366..31703)
                     /locus_tag="OO349_001263"
     CDS             complement(30366..31703)
                     /locus_tag="OO349_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5866406.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVVTLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASALWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(31778..32926)
                     /gene="dgoD"
                     /locus_tag="OO349_001264"
                     /pseudo
     CDS             complement(31778..32926)
                     /gene="dgoD"
                     /locus_tag="OO349_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
     gene            complement(32923..33540)
                     /gene="dgoA"
                     /locus_tag="OO349_001265"
     CDS             complement(32923..33540)
                     /gene="dgoA"
                     /locus_tag="OO349_001265"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HCR5866407.1"
                     /translation="MQWQTKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPPDIAVFAVGGVTPE
                     NLAQWIDAGCVGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(33524..34402)
                     /gene="dgoK"
                     /locus_tag="OO349_001266"
     CDS             complement(33524..34402)
                     /gene="dgoK"
                     /locus_tag="OO349_001266"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HCR5866408.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     VVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVAAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(34399..35088)
                     /gene="dgoR"
                     /locus_tag="OO349_001267"
     CDS             complement(34399..35088)
                     /gene="dgoR"
                     /locus_tag="OO349_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HCR5866409.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            35366..36022
                     /locus_tag="OO349_001268"
     CDS             35366..36022
                     /locus_tag="OO349_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5866410.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(36068..36880)
                     /gene="yidA"
                     /locus_tag="OO349_001269"
     CDS             complement(36068..36880)
                     /gene="yidA"
                     /locus_tag="OO349_001269"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HCR5866411.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(36994..37392)
                     /locus_tag="OO349_001270"
     CDS             complement(36994..37392)
                     /locus_tag="OO349_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HCR5866412.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQRNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSAQANNDLLSAGMELLKGKLFR"
     gene            complement(37632..40046)
                     /gene="gyrB"
                     /locus_tag="OO349_001271"
     CDS             complement(37632..40046)
                     /gene="gyrB"
                     /locus_tag="OO349_001271"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HCR5866413.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(40075..41148)
                     /gene="recF"
                     /locus_tag="OO349_001272"
     CDS             complement(40075..41148)
                     /gene="recF"
                     /locus_tag="OO349_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HCR5866414.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMDDTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(41148..42248)
                     /gene="dnaN"
                     /locus_tag="OO349_001273"
     CDS             complement(41148..42248)
                     /gene="dnaN"
                     /locus_tag="OO349_001273"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HCR5866415.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNLLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(42253..43656)
                     /gene="dnaA"
                     /locus_tag="OO349_001274"
     CDS             complement(42253..43656)
                     /gene="dnaA"
                     /locus_tag="OO349_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HCR5866416.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAACQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            43953..44033
                     /locus_tag="OO349_001275"
     CDS             43953..44033
                     /locus_tag="OO349_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5866417.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            44263..44403
                     /gene="rpmH"
                     /locus_tag="OO349_001276"
     CDS             44263..44403
                     /gene="rpmH"
                     /locus_tag="OO349_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HCR5866418.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            44420..44779
                     /gene="rnpA"
                     /locus_tag="OO349_001277"
     CDS             44420..44779
                     /gene="rnpA"
                     /locus_tag="OO349_001277"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HCR5866419.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            44743..45000
                     /gene="yidD"
                     /locus_tag="OO349_001278"
     CDS             44743..45000
                     /gene="yidD"
                     /locus_tag="OO349_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HCR5866420.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            45003..46649
                     /gene="yidC"
                     /locus_tag="OO349_001279"
     CDS             45003..46649
                     /gene="yidC"
                     /locus_tag="OO349_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCR5866421.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISSFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIVAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            46755..>48089
                     /gene="mnmE"
                     /locus_tag="OO349_001280"
     CDS             46755..>48089
                     /gene="mnmE"
                     /locus_tag="OO349_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HCR5866422.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVMADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGR"
BASE COUNT        11926 a        13011 c        12176 g        10976 t
ORIGIN      
        1 gcggtaaggt cgctgcggtt taaaaaacga aacgctatcg ataagaataa aaaggaataa
       61 aagtggaata taaagtctgg cacttcttac tgaccacgca agcgcgtttc gtacagcatg
      121 atgagagcga tgaaagcaaa ctacacttat gcttcattcg atacacattt gtaaaaggtt
      181 gaatccatga aaccaacgac actactgctt attttcactt ttttcgctat gccaggcatc
      241 gtatacgccg agtcgccttt tagctcactg cagtccgcaa aagagaaaac cacggtatta
      301 caagatttac gtaaaatttg cacgccacat gcgtcattat cagatgaagc gtgggaaaag
      361 ttaatgttgt cagatgaaaa caataaacaa catatacgcg aggccatcgt ggcgatggaa
      421 cgtaataatc agagcaatta ttgggaagcg ttgggtaagg tagaatgccc ggatatgtaa
      481 gaacagcaac aactactgac caacgcttct ttacctgata tcaggatttc ttcattttca
      541 cctttgcagt aaccaacaat gcagtcagca acatcaatgt gccggacaac atcaacggcg
      601 aagtcagacc aatattatcc agcgcataac cgccgattgc cgcgccacag gtattagcaa
      661 gctgaataac cgccacctga atagacccgg ctttttctgc ctgatcggcc agcgagcggg
      721 tgatccacgt tgaccagccg acgggaacca atgcaaaagt tagcccccag ataatcgcca
      781 cgccggtagc aacgatttta tcgcttcccc acaacgtcag taccaacgca ctcacagcca
      841 gtattaacgg cgcgcctgct aaggccagtt ttaccgaacg tttaagaatg aacgacgaaa
      901 gcgacgtacc aataaagctg gcgataccaa aactcaacag caccagcgtt aagccatcca
      961 cgccgaatcc cgccaggttc atatacaccg ggcgaatata cgtgaagaaa gcaaactgcc
     1021 cggcgaaaga catgaagatg gcgatcatcc ctgccatcac acccggacgt tgtaataagc
     1081 ggaaagtatt ttgtttctga tgcgagggtt cgcctggcag tgaaggcaat gatttgataa
     1141 tccagaaaat acacagcacg cccatcaccg ccgccgcatt aaagacattg cgccaaccga
     1201 taagctcgcc taaaaaactg cccaacggcg cggcaatcac cagcgcaata gaaaccgcgc
     1261 cgaagatcac cgacagcgcc ttcggcaccg tacgcggcgg taccagacgc atggtcagcg
     1321 acgccgacat cgcccagaac ccgcccagcg ccagccccag acaggcacga ccgattaaaa
     1381 gcaaactgaa tgagttagca aaggaaacca gcaagcagga gagcgtcagc aaaacggcaa
     1441 acaaaataac aacgtagcgg cggtcagtgg cctgaattgt ctgggtaata aacaaactgg
     1501 caaacattgc cacaaaggcg gtcacggtca ccgattgccc ggcaacccct tccgaaatgc
     1561 ctaaatcctg ggccattggc gtcaacaaac tgacgggcaa aaactcaacg ataatcagac
     1621 aggcgacaca aaacgccacc gagaaaacgg ctgaccagtt cggtcgggtg atggcatccg
     1681 cgccgcggtt ttcggcaata aattcactca tggtgttacc cgtggcatgt ttcaggggaa
     1741 aagccgacca ctttaacatt gaaatgtgtg acgaatttaa cgtttttgca acttttacgg
     1801 tgttgcttga gctggagtta agatcgaaac ggaacagggc aaagattacc ggatgcggcg
     1861 tgaacgtctt atccacccga caggtcaggc gcggtctgta ggctgataag acgaaacggt
     1921 ttcaatgcaa ccagtgaacg ccctcggcag gctgaaagaa cgcgttatag cgcatctgaa
     1981 aagcattaat atcctgcatt tcaggttcgg tttcacgcag aaaacccagc gccagcctga
     2041 cctgagcatc ggtaatcggc gcggaaaggc gggctttttg taaaatccgg tgccattgca
     2101 gcaggttctg ttcgctatca tcgacaatga caatttgcca gataagcaac acctgcgcgg
     2161 cgttttgcag gtgcgactca tccgggcgat gcaaataacg taaaaattcg tcaccggctg
     2221 actttcccat ttgatcgacc atcgactcca cgggtacgac atcgatattc agccgatcgc
     2281 tgagacggcg aatcgctttt ctggccccgg atgtcagcac ccgaaaacca accacaaaca
     2341 ctaccgccag cgtggcgagc attatccaga tcatgagttc tcctgcgtaa tcagaactca
     2401 tgatatctgg aaaccctcgc cagttactaa tccaccagaa taatcttcaa tgcgaacaga
     2461 gctgcgacca ccaccacaca caggttcaga tcgcgccagc gcccggtgca cactttcatg
     2521 atgcagtacg acataaagcc gagtgcaatc ccttcggtga tcgagaaagt aaagggcatc
     2581 atcaccgtgg taataaacgc aggcaccgat tcggtaaaat catcccagtt aacgcgcgcc
     2641 aggctcgaag tcatcagcac gccaacaaag attaacgctc cagcggttgc gtaaggagga
     2701 accatcgcca ccagcggtga gaagaacata actaacagga acataacgcc gaccacgaca
     2761 gccgttagcc ccgtgcgacc accgactgcc acaccagaag tactttcaat ataggcagta
     2821 acagacgagg taccgataaa tgcgcctgcc accgaactga cgctatcaac atacagcgcc
     2881 ttattcatat tggggaattt accgttgcta tctattaagc ccgctttatc agttacacca
     2941 attaatgttc ctgatgaatc aaataggttg atcagcataa aggagaaaat gataccggcg
     3001 agttcaagtg ttaacgcgcc gctcaaatct acttcaccaa tgacgccgct aatatcaggc
     3061 ggaatggaat agacgccgct aaaatgaaca tcaccgaaaa ataatccaca gcaagacgtc
     3121 accacaatag aaaccagcac cgcggcatga aaatgacgtg atgacaacac ggttataata
     3181 aaaaacccta aaatacctaa caacacgccg tgagaactta aatcgccaat catcaccagc
     3241 gtgtctttat tggcgacaat aacaccagtg ttttttaatc ccattaaggc aataaataat
     3301 ccgattccac tggtgatacc aatacgtaaa cttaatggaa tgttggagat catccagtac
     3361 cggatacgaa acagcgtgag caaaaatagc ccaattgcgc cccagaatat cgcgcccatc
     3421 ccggtctgcc aggagatccc catcgccccc acgaccacga acgcaaagaa ggcgttcagc
     3481 cccattgccg gagccagcgc cacgggtaag ttagcaaata tccccatcgc aatactgccg
     3541 ataccggcaa tcaaacaggt ggtaacaaac accactttcg ggtccatttg tgccgcgccg
     3601 aggatttgcg ggttcacaaa aacaatgtac accatggtta aaaaagtggt catccccgca
     3661 atcaattctg tgcggacggt ggtcccatgc tgaggtagtt taaataatcg cgaaagcgtc
     3721 cctgattcat tactcacgta atcggtattg tcattattca tttttttgtc cattggagga
     3781 gatttaatcc cttgcttttg taaaacaaat gacaacaacg gttcagtgat aattatcaca
     3841 tttcaattgc acattaatag atattcttta ataatctcgc gacgtttctt tatgataaat
     3901 aataatcaaa ttgataaaat caaaatgaga aaaatatgaa taattccatt aaccataaat
     3961 ttcatcacat tagccgggct gaataccagg aattgttagc cgtttcccgt ggcgacgctg
     4021 ttgccgatta tattattgat aatgtctcta ttctcgacct gatcaatgcc ggagaaattt
     4081 ccggcccaat tgtgattaaa ggacgttaca ttgccggtgt tggcgcagaa tacgctgata
     4141 ctccggcttt gcagcggatt gatgctcgcg gcgcaacggc ggtgccaggg tttattgatg
     4201 ctcacctgca tattgaatcc agcatgatga cgccggtcac ttttgaaacc gctaccctgc
     4261 cgcgcggcct gacgaccgtt atttgcgacc ctcatgaaat cgtcaacgtg atgggagaag
     4321 ccggattcgc ctggtttgcc cgctgtgccg aacaggcaag gcaaaaccag tacttacagg
     4381 tcagctcttg cgtgcccgct ctggaaggct gcgatgttaa cggtgccagt tttacccttg
     4441 aacagatgct cgcctggcgg gaccatccgc aggttaccgg ccttgcagaa atgatggact
     4501 accctggcgt aattaacggg cagaatgcgc tgctcgataa actcgatgca tttcgctacc
     4561 tgacgctgga cggtcactgt ccgggtttgg gtggcaaaga acttaacgcc tatattgctg
     4621 cgggtattga aaactgccac gaaagttatc agctggaaga aggacgccgg aaattacaac
     4681 tcggcatgtc gttgatgatc cgcgaagggt ccgctgccag caatctcaac gcactggcac
     4741 cgttgatcaa cgaatttaac agcccgcaat gcatgctctg taccgatgac cgtaacccgt
     4801 gggagatcgc ccatgaaggg catatcgatg ccttaattcg ccgcctgatc gaacaacaca
     4861 atgtgccgct gcatgtggca tatcgcgtcg ccagctggtc gacggcacgc cactttggtc
     4921 tgaatcacct cggcttactg gcacccggca agcaggccga tatcgtcctg ttgagcgatg
     4981 cgcgtaaggt cacggtgcag caggtactgg tgaaaggcga accgattgat gcgcaaacct
     5041 tacaggcgga agagtcggcg agactggcgc tgtctgcccc gccatatggc aataccattg
     5101 cccgccagcc agtttccgcc agcgactttg ccctgcaatt cacgcccgga aaacgctatc
     5161 gggtcattga cgtcatccat aacgaattga ttacgcactc ccgctccagc gtctacagcg
     5221 aaaatggttt tgatcgcgat gatgtgtgct ttattgccgt acttgagcgt tacgggcaac
     5281 ggctggctcc ggcctgtggt ttgctcggcg gctttggcct gaatgaaggc gcgctggctg
     5341 cgacagtcag ccatgacagc cataatattg tgatgatcgg tcgcagtgcc gaagagatgg
     5401 cgctggcggt caatcaggtg attcaggatg gcggcgggct gtgcgtggta cgtaacggcc
     5461 aagtacaaag tcatctgccg ttacccattg ccgggctgat gagcaccgac acggcgcagt
     5521 cgctggcgga acaaattgac gccttgaaag ccgccgcccg tgaatgcggt ccgttacccg
     5581 atgagccgtt tattcagatg gcgtttcttt ctctgccagt gatccccgcg ctaaaactaa
     5641 ccagtcaggg gctatttgat ggcgagaagt ttgccttcac tacgctggaa gtcacggaat
     5701 aataaaaaaa gcccggcgtc atgcagggca aaagtcacca gttacgttta tgccactgtc
     5761 aactgctgaa tttttttctc gcggcggatt ttgcgttctt ccattaccgc cactatcgcc
     5821 atcaggcaga tacaaccaat cgcggcgata tccagcgcgg cgaaggtgcc tgcccagccg
     5881 gtaaggccga ataccggcgt cccatcggca atcattccca gacctaactt ggcaaagctg
     5941 tcaccaatca ggtaagcaaa ggtgccttta ataccatcgg cagcgccaat cgctttttta
     6001 ggtacaaagc caacagcagc cacaccaatc aacaattgcg ggccaaagac caggaaaccc
     6061 aacgcaaaga gagaagccag atagatatat tcgttactgg catgttgata cacaccgagc
     6121 gtggcgataa tcagcgccag cgcgatgcag gccaccaggc cacggcgacc gttcgccagg
     6181 tcagagagcc agccccacag cagcgtaccg accagcgcac cagcttcaaa cagcgtaaag
     6241 ccctgaatcg ccaccgcttt agagagtttc agttcctgga acgcgtatac ggttgaccac
     6301 tggtcgatac caatacgtac cacatagagg aaaatgttgg cgaagcacag cagccagatc
     6361 actttgtttt tcagcacata ctcaacaaag atctgccact tggtcatatc ggtagattct
     6421 gtctctttgt cctcttcgct gatctcctcg ccgaacagtt cttcagcttt gccgaggcca
     6481 taagattccg gggagtcgct gccgtaacgc aggccgataa aaccgacaat cagcgcgata
     6541 atcgacggga agataaacat gccgatgaca tggccatcga acaggtaatt tgccccgaac
     6601 agcgccacac ctgctgcgcc tgcaccgcca aggttgtgag aaatattcca gaaaccgagg
     6661 aatgtcccgc gtttacgacg cggcgtccat ttggtgatgg tggagtaact gcacgaaccg
     6721 ccggtactct ggaaaaagcc gcttaaggcg tagaaggcaa tcatcaggaa caggctaacc
     6781 gagccgctgc ccatactggc actgaagccc agcatacaaa tggcagagag gataagcatg
     6841 aacggcagga attgtttggt gtttttgccg tcggcgtagt atgaaaccaa cgttttaccc
     6901 acgccataag tgatggagaa acccaggccg atcatcccca gctgcgtcat gctcaacccg
     6961 taggtcgaaa tcatatcgtt ctgcgcgatg ttaaagttct tgcgaatcag gtacatcgtc
     7021 aggtagccga taaagaccac caggtaggat tgcatgaacg gtttgaacca cattttgcgc
     7081 cgcacttcga gcggaaggtc cagggtcggc ttgcgaacct ggtttaggaa agccagcatg
     7141 ggttactcct gaaatgaata cctgcgtgag gcatgcattg taaaaatcag cagcaaatca
     7201 ggcctgagat agcgtccaag aaaaacctgg aaaatttctt agttttgccg gatatgaagt
     7261 gaaaaggtga gatgcatcac gcttcacgcg gtgtctgggc gttcaaaaag ggcagtaaca
     7321 gcagtgcgga aatcccggcg gcgatagaga taaccacaaa aaatccgctc cagtgccagg
     7381 tatcgagcac tttcgccagc ggccaaccag caagcgacgc ccccagataa gcaaacaagc
     7441 cgacaaaccc cgtcgccgcc cctgccgcct ctttgtggga acactctgcc gccgccatac
     7501 caattaacat ctgcgggcca aagacaaaaa aaccaatggt gaagaagcag gttgcctgca
     7561 tcacgtagct ggcaaatggc atcagccaca gcgagccgac tgaaagcaaa attccggcgg
     7621 cgaaaatcaa attcatcggc ccacggttgc cgttaaacaa tttgtccgag ccccaaccgg
     7681 ctaccagcgc accgataaat ccgcccagtt caaacatcgt cactgccgta ttcgccgtga
     7741 ccagatcgac gcccagcgtc tcggacatat acaaattgcc ccagtcgttg atcgccgccc
     7801 ggaccacata gaccagcaca tagcaaaacg aaagcagcca gatatacgga ttcagcaaca
     7861 catatttggt gaggatctct ttacgcgtca accctgcccc ttcttgttgt tgagcaattt
     7921 ccagcgcgtc gtgccgccat tcaccgaccg ccggtaaacc taacgcctgc gggcgatcgc
     7981 gtagccgcca gcagagaaaa atccccacga ctatcgccat acaaccagca atcatcatcc
     8041 cggcacgcca gccgtaatgc agcgcgctcg ctgccatcac aatgggaatg agtgcgccgc
     8101 cgacgttatg cgccgtgttc cataatgccc accaaccgcc gcgctcggta cgtgaatacc
     8161 aggccgttaa cagacgcgca cacaccggtg aaccccagcc ctggaaaaag gcgttcagca
     8221 cccagagcac ggcaaacgcc catagcgacg tcgagaagcc aaacagaatg ttcatgatgc
     8281 ccgtggcgat aagccctatc cccataaaat aacgggcatt tgagcgatcg ctgacaatgc
     8341 cggagacaaa cttcgacacg ccgtaagtaa tgtaaaacag ggtcgctaac aggccgatat
     8401 cgctacggct gagcacgccg ttagcaagga tttctggtac cgcggcgtta aaacttttcc
     8461 gcgtgaagta aaacagcgcg taacccagcc agatggtcag cagaatatgc cgacgccagt
     8521 agcgataacg ggcatcaatt tcatgtttat cagtcattaa tggcgcatcg gcaggcgctt
     8581 tcagaaacgg caacatcatc gaaccttaga cataacgttg aggtagagaa acgctgacac
     8641 gcgtgccgtg cagacaggaa atgtgtaatg tgccgcccag cgccgttacg cgctcgcgca
     8701 ttccggtgag gccaaaacct tgttgcccgg aacccggcgg caaaccgctg ccatcgtctt
     8761 caatcaccag catcaaccgt tcatcctgct gccagccttg cagcgtaacc gcgctggcat
     8821 cggcatgttt cacaatgttg ttcagccctt cctggcagac acgaaacagc gtcacgcgct
     8881 ggttttcgct taatgctgat tcatcgatac gccattcgag atggctgaca atgccgcgcc
     8941 cttccagctc catttcccgc atcagtgagc ggatggcctg ctccagggtg agatcatcca
     9001 gctggcgcgg acgtaaccgt cctaacaaac ggcgcaccgc gtcgtaaacg cccagcgaca
     9061 gttgttcgat gagctgcccg ctctgcttca cactggcgtt atctgccgcc agccgctgaa
     9121 caatgcccgc ctgagtacga atagcagtga tggtctgacc gatatcgtcg tgcagctcac
     9181 gcgccacgtc gcggcgcacg ctctcttcgg tttctaacaa acgttcagcc agatgctgat
     9241 tgcgcgccag ttccttttgt agcgactggt taagttcacg caaccgctgg atgcctgcgc
     9301 ccagtaacaa ccctgtcaga ctttgcacca gcaacgagag taataaatcc accagatgat
     9361 cgcgccaggt ttgactggcg atcagcgcga tggcgttcat caacgtcgca atcagcgctc
     9421 cttgccagcc atagtgccag gccagcgcga taatcggcag cgccagacag aatggcgtaa
     9481 agcgcgacag ttcgtccggc aatcccaact ggagccagag actgataaca aacagcagca
     9541 agtaccagac cagatgccgc ccgcgccagt tgatgggctg agaaaccagt gacgggccga
     9601 gcggcagcca ggtgttattg gcgagatagt gccagaacac cagacatatc ggggccagcg
     9661 tcagaccgcc agttaaagtc agcaacagcg cattccacga ctctttgccg tgccaaagcc
     9721 agggcagcga ctgcaacaac gccgccgccg ttaacgccgc accctgtaac agcaaggtgc
     9781 gccagtcacg ctgatggcga tagcgcgaga tcagcgctac gggcagtaac gtcagtaaac
     9841 taccgatcat caataacgga aaatgggtta aaccgaccgc ctgcgttagc cagtaaatca
     9901 gcagccactc cgcacccagc aatacgggcc agtatccgcg cgggcattgc agcattagcc
     9961 ccagacgcag accaaacgga aataacagca ccgccatatc agggcgctca accagatgca
    10021 ggctgatact ccataggcaa aaccatgcgg cagagaagat aaaaaagcag gcaataacgg
    10081 taattaagcg ggagaacaac gtcttcatca ccagccatca aacatgcggc gcgccagctc
    10141 tacgtcgtta ctgacgccca gtttttccat cagattggcg cgatggacgt gtaccgtttt
    10201 cggtgacaag cccagttcgg cggcaatctc tttcaccgcc attccttgcg ccagtttttc
    10261 cgccacctgg cgttcacgtt tggtcagcgg gtcctgacga ccggatgcca gtttaatggc
    10321 aatatccggc gtcagataac agccgcccgt ggcaaccgta tgcaccgcag caatgagttc
    10381 atccgggcta cagcgtttgg aaagaaagcc gcgtgccccc gcgttaagcg cctgctcaac
    10441 cagcgcagga ctgtcgtgaa cggagagcat aatcgtcgcc atacctttcg gcagctggct
    10501 tagcagctcc agaccggaga tatcgggcat ggagatatcg caaatacaca cctgcacacc
    10561 gcgccccggc agccccgcca gcgcctcgcg ccccgaacca aactcggcaa ctacctgcaa
    10621 atcaggttcc agccccagca gctgcgcaaa gccggagcgg acgatgaggt gatcgtctat
    10681 aagggcaacg gtgatcatgg tcttgtcctg gcgggtaaaa aaaaacgcgc ttaccttaac
    10741 gataagcgcg atgttgttca agccttgagc ggttactgaa aaaacaccgc gatcttgtta
    10801 aacatcgtcg gatcggactg attacgctgc actttcacga catcttccag cttagcgatt
    10861 tggcttatca tctgctccag acgctggtcg tcattgacca gtagccagat atggcttttg
    10921 tcgctgtcct gaatcggcag acaaagaatg ccttcaacgt taaaagcgcg gcgggcaaaa
    10981 aggccacaaa cgtgggtcat tacgcccgga tggttgcgaa cggtgagctc cagaattacg
    11041 ttgttatgag ttgtgttttg catggcttat tcccccacca tttcagtatt cgccgcacct
    11101 ggcggcacca tcgggtaaac tttttcttcg gcatcaatgc gcacatggat cagcgccggg
    11161 ccagggcgat tgatgatttc ctgcaatgca gcctgcggat cggcttcgtt attcaaatca
    11221 caggtttcga ggccgaatcc ggcggcaatc tgcataaagt tgattttgcc cggataggtg
    11281 gcggcaaaaa cgccttgctc gtagaacaga ctctgttgct gatgcaccag ccccagcgct
    11341 tcgttgttca tcagaatgat tttgacatcc agctgatttt cactggcggt cgccatctcc
    11401 tgaatattca tcatcaggct gccgtcgccg gagaaacaca acactttgcg atccgggttc
    11461 gccagcgccg cgccaatcgc cgcaggcagg ccaaaaccca tcgtgcccag cccaccggag
    11521 gtcagccact ggcgtgggcg attgagcgga taagcttgcg cggtccacat ctgatgctga
    11581 cccacatcgg tggtgataat cgcattgtca tcgacacagg cggcaacggc gttgatcagg
    11641 ccgtaatggc ttaatggatc gcacgctttc gggattggac acgggaattc gcgctgcaaa
    11701 tccgctacca actggtgcca ctctgcacgc ggttgcgctt ccacccgcgg gatcaattgc
    11761 gccagcacgt catcaacatc cgcctgaatc gccacatgcg gctgcttgat tttacccagc
    11821 tctgcgcgat cgatatcgac atgaataatt ttggcattcg gacagaactg ctcggttttg
    11881 ccaatcgccc ggtcatcaaa acgcgcaccg agcacaatca acaaatccgc ctcctgcaaa
    11941 atatagttgg tactgcgcac gccgtgcatc cccagcatac ccagcgacaa cggatgcgct
    12001 tttggcagca tgcccagcgc cattaaagtc atggtggtag gcagttgcgc tttctccgcc
    12061 agttcacgca cccgcgcagg cgcattgatc acaccaccac ccagataaag caccgggcgt
    12121 ttggcagcgt taatcattgt agctgcgtca cgaatgcttt cttcgctaaa ggcgggtgca
    12181 gcggcttttt ctgccaccgc gggctgagct tcaatctcaa aaaccgccgt ttgtacatcc
    12241 ttaggaatgt ctatccacac cgggctaggg cggcctgatt gcgcaatgcg gaaggcatcg
    12301 ctcatgacct gtgggagctc ttcgatatgt ctgaccagat agttgtgttt ggtgatgggg
    12361 atagagatgc cgtaggtgtc cacttcttgg aaggcgtcgg tgccgatcat cgaggcggga
    12421 acctgaccag tgatgcaaat cagcgggatg gagtccagcc gcgcatcagc aatggcggtc
    12481 accaggttag tcgcacccgg cccgctacag gccatacaga ccgccggttt accgtcggtg
    12541 cgcgccattc cctgagcgat aaaccccgcg ccctgttcat ggcgagccag aatatggcgg
    12601 atttgcgtgc tttggcttaa ggcgtcgtaa acaggcagga tagaaccgcc cggaatgccc
    12661 gtcacaatct taatgccctg ctgttccagg aaatgaacga taaattctgc gccggtaaag
    12721 cgcttacgcg tcgatgttgt gcccgaactt gccatgctcc agtccttttc ttctgggccg
    12781 actttccggg aggtgcttaa acgaaaaacc ccgcccggtt tgcgccggcg gggttttgga
    12841 atcgtgtgtt gttccagtcc ctacggcgca ttgccgacga ccaccaccac acgcacgacg
    12901 accactgcgg cggatggcgc agttggtagt agttttgcgt tgaacatgga agtagtcatt
    12961 ggggaccttg ttggttttgt gtttaacaat atttatacaa gcacagcttt acaggggaga
    13021 caatggaaaa tttttcagca agggaaaatt gaggggttga tcacgttttg tactgaattg
    13081 cagataacaa aaaaccccgc cggagcgagg tttcgtcagc cgcctgcggc tggtaaccgc
    13141 aaagcacact gtattatgtc aacactgaaa gtatacgtgt tccgcgcaga acgcgcaatt
    13201 tcggcacgaa ttttgacgta tttagtgcat agttgagtat cgttcacagt ttgcgttttg
    13261 tccaaatatt actgtttatt tatacagtaa acttctataa tatcactgta cgcaatgtgt
    13321 tatgcggggg ccgcatcgtt acccggcgca ctaagtcctg gctgaaacgg gtggtgccgt
    13381 cagcgcctta accccgcgtg agcacactgt gttatgtcaa caagcacaac gtttctcctt
    13441 gagataccgc gtgcacaaca gctggcaaca ggcagcggaa aggtacgtca gctggcagtg
    13501 ctcctgaacc acaggagacg cgtatgagcc tggtggatat cgccattctt atcctcaaac
    13561 tcattgttgc agcactgcaa ctgcttgatg ctgttctgaa atacctgaag taattcagat
    13621 tcaagtcgca ccaaagggga gcgggtaacc gctccccttt tatatttagc gtgcgggttg
    13681 gagtcggatg cgatgctgac gcatcttatc cgccctacca tccctcccgg caacatttat
    13741 tgccgctttt gtttacatat tctgccgcta aacaattccc cattcctggc gtatatctgg
    13801 ctaacattca tcaatgtgat agattcctct cccgcattta tgggaatgcg tagtgactta
    13861 ttctaattat ttttataaaa gcatccgtga taatgaaaag gcaaagaaac gtcaatttgt
    13921 tattgatgtt ggtattactc gtggccgtcg gtcagatggc gcaaaccatt tatattccag
    13981 ctattgccga tatggcgcgc gatctcaacg tccgtgaagg ggcggtgcag agcgtaatgg
    14041 gcgcttatct gctgacttac ggtgtctcac agctgtttta tggcccgata tccgaccgtg
    14101 tcggtcgccg accagtgatc ctcgtcggaa tgtccatttt tatgctggca acgctggtcg
    14161 cggtcacgac ctccagtttg acggtattga ttgccgccag cgcgatgcag gggatgggca
    14221 ccggcgttgg cggcgtaatg gcgcgtactt tgccgcgtga tttatatgaa cggacacagt
    14281 tgcgccatgc taacagcctg ttaaacatgg ggattctcgt cagtccgttg ctcgcaccgc
    14341 taatcggcgg tctgctggat acgatgtgga actggcgcgc ctgttatctc tttttgttgg
    14401 ttctttgtgc tggtgtgacc ttcagtatgg cccgctggat gccggaaacg cgtccggtcg
    14461 atgcaccgcg cacgcgcctg cttaccagtt ataaaacgct tttcggtaac agcggtttta
    14521 actgttattt gctgatgctg attggcggtc tggccgggat tgctgccttt gaagcctgct
    14581 ccggcgtgct gatgggcgcg gtgttagggc tgagcagtat gacggtcagt attttgttta
    14641 ttctgccgat tccggcggca ttttttgggg catggtttgc cgggcgtcct aataaacgct
    14701 tctcaacgtt gatgtggcag tcggttatct gctgcctgct ggctggcttg ctgatgtgga
    14761 tccccgactg gtttggcgtg atgaatgtct ggacgctgct cgttcccgcc gcgctgttct
    14821 ttttcggtgc cgggatgctg tttccgctgg cgaccagcgg cgcgatggag ccgttcccct
    14881 tcctggcggg cacggctggt gcgctggtcg gcggtctgca aaacattggt tccggcgtgc
    14941 tggcgtcgct ctctgcgatg ttgccgcaaa ccggtcaggg cagcctgggg ttgttgatga
    15001 ccttaatggg attgttgatc gtgctgtgct ggctgccgct ggcgacgcgg atgtcgcatc
    15061 aggggcagcc cgtttaagcg cacgtcaccg cagcatcgtc atcagctcca tgggagaacg
    15121 atgctgcttt atcagatcac gcatcacccg catatgcggt gcggagtaag aataaaacgc
    15181 ctgatagccc gcacaaagca cgctgtctgg cgtctcctgc cgagcgtgcg ggcacaggcg
    15241 atgccagtcg cacgcctggc agtcacggcg cggttgcggg ttttcgctgc cacaccattt
    15301 ttgtaaggtg gcgtcaaaaa gctcaatcga gatacgactg atatctgccc gcacccaaca
    15361 ctcaaacagg gcaattaaaa atttccccca cagctctgct gtcaccgaag cggccgtcag
    15421 atcgccccgg tcatcaaatt caagataggg aatgagctgt acggagcgga tgccccgctg
    15481 tttgaaattg tgataaaaat cggcaggttg cagagcatcc ttgtactcta caaccagctt
    15541 atgcctgtcc tcttcagcat caatgacctg acttcctgtc atgcgactct ctcaataaat
    15601 acgataagtt gatgtatatg cgttacagca aacagtattg cgagagataa caccgcgagg
    15661 gagatcaaaa atttgtcacg gactacgtga aattgggaaa aatcgctatt cgtgacatcc
    15721 attaaattgc gattacgggt gtagtgccag aggatcagcg ccacgatggc gaggatgcca
    15781 atcgaaatcc agaataacat gcccgcctgg tgccagttgt gtttgatagc caacgccatc
    15841 aacgcgccgt agcccagcat ggtacgaaac cacgccagtg atgtgcgctc cggctgcagc
    15901 cctgggtcgg caatgcgtct ggctttacgg ctatccggca tacaacacca gtcccataac
    15961 gatcaccacc acaaccatca atattaagct gatgattaat aagctgttgg tgtagggcag
    16021 gtcctctttc agacgcatcg ctttttcatt gcgtaaccag cgcaggtagc catacattgc
    16081 taacccgccg gaaaacagac acaacaacaa cgccagcagt tcacgaatga cgggcgtggc
    16141 gaaatctggc gcaagttgat caagcccaac ccccgccgcc agaaatccta gtgcggtacg
    16201 gatccacgcc agaaaagtac gctcattagc cagcgagaag cggtaatcgg gcgcttctcc
    16261 aaggcgggaa attttcattg ttgctccttt gcaatttcac taacgctcta tctttagcgg
    16321 ttttttttaa gaatttcgtc cgcccgtttc ctgatatgct tttgccatcc gctacaagga
    16381 ggtcagatgg gtatcatagc gcaaaataaa atcagttcat tgggcatgct gttcggtgcc
    16441 atcgctttaa tgatggggat tatccacttt tcattcggcc ctttttccgc accaccgcca
    16501 acgcttgaaa gcatcgtggc ggacaaaacg gctgaaatta aaagaggtct tctcgccgga
    16561 ataaaaggcg aaaaaattac cactgtggag aaaaaagagg acatggatat cgataaaatc
    16621 ctcgatcaaa gcggtatcgc actggccatt gctgcgctac tttgcgcatt tattggcggt
    16681 atgcgtaagg aaaatcgctg gggcattcgc ggcgcgctgg tttttggtat cggcatcgtg
    16741 tgcagcattt tgctgatatt cctgattttt tccttcctga ccggcggttc gctggtttaa
    16801 gcagcctgaa taatgcccgc cgggtgatca tcagcgggca ttatcgtcag aatttctgtt
    16861 ttttctcctc caccttcact ccttgcgtcg gtaggccggt gtcatagtcg cgtaccactg
    16921 gcgaatagcc atcttgtggg cgtgggcgaa acgcccccat ccagcgcggt cgtgcatctt
    16981 tacgccacgg acgcagactc cattggtaac tgcggaacgg atcgcgaatt ttgtccatgt
    17041 aatccaataa ggcgtcatgc attttgctgc gaacgtctgc aaaacggata tcatcgatca
    17101 ggttatgcat ttcatttggg tcattacggc gatcgtaaag ttcatcactg gtgaagaggt
    17161 tgagcaccag tttaaagtca tccgtcaccc agcaacgcac cggaataaaa ccgccaaagc
    17221 tgtcatgctc aatctcgtag cggttaaatt ccaccatcac gccgcgtggc tctttcacgg
    17281 caaggatatt ttcccccggc agaatctctg gtttttcaat atctgccagc gccatcattg
    17341 tcggcagtaa atcaatatga ctgactggcg tatcgacctg tcgccgctcc ccttgcggcg
    17401 aacggatgat cagcggaata cgggtgatgt cgtcatacat cgccgctcct ttactgatca
    17461 gcttatgtgc gcccatcatt tcaccgtgat cggaggtata aatgacccac gtattttcac
    17521 gttgctctgg cgttaaggca ttgatgacac gtccgatttg gtcatcaaca aagtcattgc
    17581 aggcaaaata gagcggatgg tgataaagcc cgtcatcacc gactggcgat ggcatcgcct
    17641 gcgcccataa gcggtgatgt tccggtttgt tcgccaggtc atcctgtgat ttctcgccaa
    17701 gttcgtagta aaaatcagcg tatttctcta aatactccac cggacaggtg aacgggtgat
    17761 gcggctcatc atacgaaacc accatcagga agggttcctc ggcgcgcacg ggctattgca
    17821 gaaaatctac cgctcgattg ctgatgcggt gcgcccaggt gaaggtttcg tcaatatggt
    17881 tcgcctgtaa atcttcgacg ctgtttaggc cattgcgcca caggctgatc tctttttccg
    17941 tcagttcgct aagatagttc gccccatcaa accagtaatc agcatcccac tccggcggac
    18001 actcgccagt gccgaaatag tcatgaccgt cgagatgcca tttgccgatg taacaggtgt
    18061 gatagccggc atccttaaag tagcgcccca tagtggagat gtttttgcct ggcgcgacgt
    18121 tgttggtcca cgggccggac tggttagcgt agataccggt gaatagtccg gcccgtgcag
    18181 gcgtacaaac cggtgaacaa gtgtaggcgg aattaaagcg aataccttcc gccgccagac
    18241 tatcaatatt ttgcgtattc agcggtttac cgctatagca accgaccata ttggtggcct
    18301 gggtatcggt caattggggc gtttcatagg ctatccttcg cgtcgggagt aaaggttacg
    18361 gctgggtcgt gacgatgcca ccagctgtca aaaatcaggt agacaatcac tacggcagcg
    18421 atgaaataac tgatcatcgc taaccagcgc gtaccgtagc cgccgaattc agccagcccg
    18481 acatagacgc caatcatcgc gaacaggata ccaattgacg cgatcttgac gtttttccac
    18541 ggtttcatgt ccaccgcaaa cgcatctttg aaggtgaacg gcgtggcgcg cggtttgata
    18601 aaaccgatca ccagcatcac gaccacgttg atgcagaacg tacaggccag cacatagagg
    18661 aaatggaagt cgaacttcac cagatagttg atggtgatgt agctgattat gccaatcccc
    18721 atcgccactt ttgccgccag cgccgggatg cgcggaaaga aaaagcccat aatgatgatg
    18781 gtaaccagcg gcacgttgta aatgccgttg agctgtttca tccagctata cagcccctgc
    18841 ggcgcgttgg cgatccacgg cgctaccaaa accgaaacga tagcgataaa gaaaccaaat
    18901 ttgcgcccga cggtgaccag ctgctgcggc tcggcattct ggttaatgat gcgacggtaa
    18961 atacccatac tgaataatgt actggcgcta ttcagaaagc cgttgaaggt actgatcacc
    19021 gcaccaaata acaccgcgcc gaagaaaccc tccagtggca ctggcaaaac gttattgacc
    19081 agcgtcgggt aggccatgtc ggctttcggt agatcctgat acagatgaaa cgcaatcaac
    19141 cctggcagca ccagtaccag cgggtcgagc attttcagca ccgccgttaa cagcgccccc
    19201 ttttgccctt ccgccaggct ttttgacgcc agcgtgcgtt gcacgatgcc ctgatttgta
    19261 caccagtaaa aggtgttcac cagaattaaa ccggtaaatg ccgcgccaat cggcaaggga
    19321 tcggtcgggc caccgactga gtttaatttc tcggcgtgaa cggtggtaat ttgctcaatg
    19381 ccctgcataa agctgccctt gcctatcgcg atcaggccaa ataccggcac catcaacccg
    19441 ccgataacca gcccaatacc gttgatggag tccgccactg ccattgcgcg cagtccgccg
    19501 atcaccgcat acaaaattcc cgccagacca agcaaaatta ccagtagcca gatagccgca
    19561 ccgtgagaaa tctgtagcga ttccccgacg tgaaacaggc tgttgagcgc caacgcgccg
    19621 gagtagagaa caatcggcag aaagcagacg ccggtggcaa tgaggaagca gaagtcgatg
    19681 ataatacgcg tcgttttatc ataacgttcc tccagaaaat cggggatggt ggcaatgccg
    19741 cgcttcagat agcgcggtaa aaagattagc gcgaggaaga tcagcgtcac cgctgaagtc
    19801 acttcccagc ccatcaccga catgccgctt ttgtaggcct gcccggaaag accaaccagt
    19861 tgttccgtgg aaaggttggt taacattaac gaagcggcaa taaccggcgc ttttagtgaa
    19921 cgtccggcaa gaaaatagcc ttgttgcgat ccggtatctg ttttgcgaac cttccaccag
    19981 gtgatcaccg ccaccagcag cgtaaaaccg acaaaactca agatttgtaa cgaattcatc
    20041 tcatagccct tatttatgtt tatgtcccgg acgcatgaat atagggagag gtaaaatgcg
    20101 tcgggtcagg cagtcagatt cttaaaatac aaacgtcgta tccctgaacg gattgttttc
    20161 tgttaagttc aggttgtgag cataattcgc cggagggata ttaaaatgaa tggaaaattg
    20221 caaagttcag atgtaaaaaa cgaaactccg tacaatattc ccttattgat taacgaaaat
    20281 gtgatctcca gcggaatttc tctgatctcg ctctggcata cctacgccga cgagcattac
    20341 cgggtgatct ggccgcggga caagaagaaa ccgcttatcg ccaactcatg ggttgcggtg
    20401 tataccgtac aaggatgtgg gaaaattctt ttaaagaatg gcgaacaaat aactctgcat
    20461 agcaactgta ttatattttt aaagccaatg gatattcact cttatcactg tgaaggttta
    20521 gtctgggaac agtactggat ggaatttacc cccaccagta tgatggatat tcccgttggt
    20581 cagcaaagcg ttatttataa tggcgaaatt tataatcagg aactcaccga agttgctgag
    20641 ttaataactt caccagaagc aataaaaaat aatctggcag tcgcttttct gacgaaaatt
    20701 atttatcagt ggatttgtct tatgtccgca gatggtaaaa aagatccaca acggcggcaa
    20761 attgaaaaat taattgccac tttacatgcc agtctgcaac aacgctggag cgtagctgat
    20821 atggctgcca cgatcccctg tagcgaagcc tggttgcgtc gtctgttttt acgctatacc
    20881 ggcaagacgc cgaaagaata ttacctcgat gcgcgtctgg atctggcgct atcgctatta
    20941 aagcaacaag gaaactcggt tggcgaagtc gctgatacgc tcaacttctt cgactccttt
    21001 catttcagca aagcctttaa acataaattt ggttatgcgc cgtcagccgt gctaaagaat
    21061 acggaccagc acccaacgga tgccagtcca cacaattaac gtaactctgg ccagtacgct
    21121 ttgttggcct ccagcaactc atccagaata gctttggcga ccgaggcgct cggtaccgtt
    21181 ttcgacaacg taatcgcctg ccacaggtgc tgatatgaac gctgttccca ggcgtccacg
    21241 accagttttt ccaccgccac ttgctgactc attaaccctt tctgaaactg cgggatatcg
    21301 ccgaccacca gtggttcagg ccgtagtagc gcacgcgcat ctgtttgcga tcttgcagcc
    21361 caacaatttg cgccatccgg ctttcaatac cgattggcat gtcacagatg ttgaggattt
    21421 tcgcattcgg gcgcagacga cgcgtggctt ctgcgacaat tgctgccggg ttggagtagt
    21481 tgagcatcta ggcgtttggt gaatattttt ccatataatc caccagttcc aggacgccgc
    21541 caatggaacg catgccgtac gctattccgc cgggtccgca agtttcctga ccaacaacac
    21601 cgtggcgcag tgggattttt tcatccagtt cgcgcatcgg gtacttgccg acgcggatgt
    21661 gcgccatgac aaaatcaacg tcgctgaacg ccacttcagg atcggtggtg taactaaacg
    21721 caatgtccgg cgctttttct ttaaggatga ctttacaggc ttcggcaatc acttcctgcc
    21781 gcgcgccatc gttatcataa aatttcagtg cacgaagcgg gaaacggtcc tgattcgcca
    21841 ggagcatcaa cacgatgcct ggcgtaaagg tgcttccacc gcctgcgaca accactgaga
    21901 atttggtcat tatactgcct ccgtaatggc aacattttct gctgattgat gagaattaat
    21961 taagctatcg agctgttcac gcaattggga tacatgcaga ccaatgatca cctgaatggc
    22021 atcgccacta cggaagacgc cgtgcgctcc cagcttttta aagacttcgt catccagcgt
    22081 ttgtgacatg tcatgcagtg caatacgtag acgcgtcgcg caattgttaa tgctggagat
    22141 attgccgacc ccgcccaggg cttgcaggat accggcagcc tgatccagct ctttttttgg
    22201 ctctgcagcg gtggtttggc ctcgcgaggc tttgtattcg gcttttgagt agagtttcac
    22261 ttccgcatct tcacgtcccg gcgtgcacat gttgaactgc agaatcaggg tgcggaaaac
    22321 cacgaagtac agcagggtaa agcacaaccc aatggcgatt tgggtcagca tcatatccgc
    22381 gtggttgctg aacatcggga tccagttttg cggtaaaacc tggtcaatca gacctccgcc
    22441 catgttgccc accacaccaa agagatacat tacggtcgac attgaggccg ccagcacggc
    22501 gtgtaccgca aacagcaacg gtgaaatgaa caggaaggta aattccagcg gttcggtaat
    22561 tcccaccagc atggcggtta aggttgcggg aatcagcaag cccgccactt ttacccgatt
    22621 ttccggtgcg gcagtgaagt acatcgctaa agaaatgccc acggcaccaa agatttttga
    22681 gttaccgtgc agggcaaaac cgccttccgg gaacaacgat ttcagtggct cggcgctcag
    22741 actgaactct tgcagatgct gcgcccagta catctgaatg ccgccttcaa cagctgccgg
    22801 accaaagata aacggtccgt agatgaagtg gtgtaaaccg gttgggatca gaatacgttc
    22861 gaggaaggtg taaacccaca ccccaagcgc acccgccgaa cgcaggaacg cttgcagaga
    22921 ttcaatcccc atttgtactt ttggccagcc gagcaatgtc agccaggcac aggggatcat
    22981 caccaggaag gcgataatca ccacataaga cgtcccctgg aaaatgccga gaaaaaccgg
    23041 cagtttttta tcgaacagac ggttatgcag cgccgtcaca atgccggaaa tgataattgc
    23101 gccgataatg ctggtatcga gggttttaat cccggccatc attgtcagac cgctacctgc
    23161 caccgcgtcc tgagtgaaat cgacgccgaa gtagcttccc caggtcattc ccatcgcatt
    23221 gatgaaatag ttccaggtca ggaaactcac catcaccgcc agacaagcac gtccctgcgc
    23281 ttgcttagca aggccaatgg gtaaaccgac agcaaaaatc agcggcatat tacggaatac
    23341 cgtccaaccg ccctcttcaa taatgtgtac gatttgcgcg aataaactgt tcggatcggt
    23401 cagtgattcc ccgacaaaac atcgggtttt gcagcaagat ggcaagaccc accacaatcc
    23461 cggcgaaggg aaacagcagc actggcgtga acatcgcgcc gccaaagcgt tgaatttgac
    23521 tgagcatttc cacatcctct tttctcaatt ctgtaagtag gaagcctgat tggttatgcg
    23581 gcagacctgg agcgaggata ggaagatgcg atcggcagaa catgacgacc caatgggatt
    23641 cgtgatcgct ttcatgcttt tcgttgggcg gtagagatct acgttttcgc gaaaaagtag
    23701 acatctgtga gcacaggtac acagcatcga acacatcttt aaaaaaaaga tttttttcaa
    23761 tcgattaagc agaacttgtg ggcgcattac ccgtgcttgc aggcaaaaaa gagagctaga
    23821 gatgatctac aaaagcattg cggagcggtt aagaattcga cttaattccg cggatttcac
    23881 gctaaacagc cttcttcccg gtgaaaaaaa gctggcggaa gagtttgcgg tatcgcggat
    23941 gactatccgt aaagccattg acctgctggt agcgtggggg ctggtggtcc gccgccacgg
    24001 cagcggcact tacctggtgc gcaaagatgt gctgcatcaa accgccagcc tgaccggact
    24061 ggtggaggtg ttaaaacggc agggaaaaac ggtcaccagc caggtgctga tttttgaaat
    24121 catgcctgcg cctccggcca ttgccagcca gttacggatt caaatcaacg agcagatcta
    24181 cttctcccgt cgcgttcgtt ttgtggaagg gaaaccgcta atgctggaag acagctatat
    24241 gccggtaaaa ctgttccgta acctttcgct gcaacatctg gaagggtcga agtttgaata
    24301 tattgaacaa gagtgcggga ttttgattgg cggtaattat gaaagcctga tgccggtgct
    24361 cgccgataga ctgctggcgc ggcaaatgaa ggtagcggaa cacacgccac tgctgcggat
    24421 cacctcgttg tcatatagcg agagcgggga gtttttgaat tattcagtga tgttcagaaa
    24481 tgccagcgaa tactaggtgg agtaccattt acggcgactc cacccggaaa aaagttaacc
    24541 gatactccag aagagcaccg ccagtaattg gggggtgata atgcgcagga acatcactaa
    24601 cggatagaca gtggcgtaag agagcgccgc cgcaccactg gttggatgaa gattattagc
    24661 aaacgccagc gccggaggat cggtcatgga acctgccagc atcccgcaca tggtcaggta
    24721 attcattttg gctaacatcc gcgccagaat gccaacagta atcagcggaa cggcggtgat
    24781 cagggcacca taaccaatcc agcttagccc ttcgccattg accagggtat tcacaaaatc
    24841 cccaccagat ttcagaccaa cgaccgagag gaacagcacg atccccagct cccgcagcgc
    24901 gaggttggca cttggcggca taaaccagta cagcttgccg atactgccga tacgcccgag
    24961 gatcaacgcc ataatcagcg gaccgcccgc cagccccagt ttcaacgcgg ccgggaatcc
    25021 tggcacaaag acgggaatag aacctaacaa tacgccaagc ccgatgccaa taaacactgg
    25081 cagcatctga acctgttgca gtttttgctg cgcattcccc agcacattgg caacggcatc
    25141 aattgcggac ggacgcccca ccaggttgag gatatcgccg aactgcaggc tgatatcgcc
    25201 actggcgacc agttcgaccc cggcacggtt caggcgcgag ataacaacgt catagcgttc
    25261 tttaaagtgc aggtcgcgaa tacgttttcc gagcacgttt tcattggtga ccaccacacg
    25321 ctcgacgcgc aaatcagtgc ctttcgtaga cagcgaggta tcgacctcct gaccaatcac
    25381 cagttgcgca ttatgtaaat ccgctggctg ccccaccaga tgcagcaaat cgcccagttg
    25441 gataatggta tctggcgaag gtacttttag ggtttcttct cgtttcagac gcgagcagat
    25501 aattttgtcg ccgttgagaa tcggtacatc tttaatggct aaatcatgca ggttagggtt
    25561 ctcaacgcga atattgatag tcttgatcaa cgcgccgcca ttggtgcgtg aagactcgtg
    25621 ctgctgagct tctgtctcga cattgacgcg gaaaataacc cgcaacatcc acatggtgaa
    25681 caaaatcccg caaatgccga atggatacgc catcgcgtaa ctcatcccca tctgatcgac
    25741 catttccatt ggtgtaccca ggtcgcgcaa gatctgctgc cctgccccca gcgctggcgt
    25801 attggttacc gcaccggaga aaatccccag cactaccggc agtggaatat caaacagttt
    25861 atgcaggatg gcggtaacca gaccaccgat gatgacgatc agaacagcaa acaggttgag
    25921 gcgtaatccg gagacgcgca atgaggcaaa gaagcccggc cctacctgaa tcccgatggt
    25981 ataaacgaac aggatcaggc caaactcctg aataacatgc agcatatcgc tacttaatgt
    26041 catccccgcc tgagaaacaa aatggccgac gatgatccca ccaaacagca cgccgccaat
    26101 acctaatcct atgccgcgaa atttgacgtt gccgataaac aaaccgacga ctgccaccaa
    26161 agccagaata ctgaccgtta atgctatatc actcatcgtc catttctctt gagataacat
    26221 tttaattgct acggattatg gctgaatcgg tgaagcagat ttgccttata gcgcacatta
    26281 tcacgggaaa gaacgtgccg aaatatctta aacagtcgaa atttaacgca ttgttgaaga
    26341 agaaagacaa aaaaagcccc accagaatgg cggggcaaag agaatagcta gttagctatt
    26401 taacgcggga cgttcgctga tagcgatacg ctgcgctgcg atgggttcag gctcattacg
    26461 aattaaatca atatgcagta aaccgtttac gaaggttgcg ccagagactt ccatattttc
    26521 agccagcgta aaactcaggc taaatggctg attcataagc ccttgatgca gccatttttt
    26581 ctcttctttt ggctgctccg gcgtgccttt tacgctcagg cgcgtacctt ccagttgaat
    26641 ctctaaatct tcctgacgga aacctgccag cgcaagggta atgcggtagt ggttatcgtc
    26701 gcttttctca atgttgtacg gcgggaagct ctggctttca ccggcgtttt gcagtgcgtt
    26761 ggccagtttg tcaaaaccga tccattgacg catcagtggg gataaatcga agttacgcat
    26821 agtcatttct ccttctaaga agcgagtaag tacctgcaaa tccgaagatt cgcatatgct
    26881 ccctgacggc gagcatggag atgtcaggcc gcgccaggcg gccttaggga attagttgat
    26941 ttcgatacgg cgcggttttt tcgcttccgg aatcacgcgt tcgagatcga tatacagcaa
    27001 accatttacc aggttagccc cacgaacatg aatgttctca gctaactgga atttgcgttc
    27061 aaagttgcgt tcagcgatgc cctggtacag ataggtgcgc tctttttgtt cgtcggcgtg
    27121 agcacctttc accaccagca gattatcctg ggcggtaatt tccagttcgc tctcagcaaa
    27181 accagccaca gcgatagcaa tgcggtaatg gttttcgtct accagttcaa cgttatacgg
    27241 agggtagccg ccattactct ggctctggtt gttttctaag tggttaaaca aacggtcaaa
    27301 tccaatagca gaacggtaaa gcggggataa atcaaagtta cgcataatca atagctcctg
    27361 aaatcagcga gaatgtaaga ccttccacaa tggacaggtc aggtagccag aacacccatc
    27421 aggcgcgtcc tcatcgacta cgatgtaaaa tgggtctgga aatgaacttt tcaagcctta
    27481 tcagcggact tttttttgca gtttatggtc tattgcatag actgaggggg cagcacgaaa
    27541 cgttaaagtg cgatagccgc cacagtgcga cgtaatgatg atgttatttt cagcaaggga
    27601 tcgctataac tcatcaggcc aacacccacg atcaacagat gaatttatta tgataagaaa
    27661 tgtgttgtta gcgttcatga tatgcagcgg aatgacatta ctcggaggat gctccagcgt
    27721 gatgtcccat accgggggga aagaaggaac gtatcccggc acccgcgcca gcgccaccat
    27781 gattggcgac gatgaaacca actggggcac taagtcgctg gcgattctcg atatgccgtt
    27841 taccgctgta atggacacgc ttttactgcc gtgggatgtg ttccgcaaag acagctcggt
    27901 gcgctcgcgc gttgaaaaaa gcgaagccaa cgcgcaagcc accaatgcgg ttattccacc
    27961 ggccagaatg cctgataact gatttagcga tcagtttcgg tgatccacag ctggccacct
    28021 tccatccacg ccagccattg accatccggc gagaagacca cggcgtccgc agaaggtgga
    28081 ttggcgtggt tttcggttaa atactcaacc tcgccgcttt gcgcatgggc acaaacaatt
    28141 cgattatcca gcacaaagcc caaccattct cctgacggat gccagttaaa tgcagactga
    28201 atatccgttt tgttatgggt taacttgcgc ggttcgccgc cctgtggcga gataagccac
    28261 agttgcacaa tgccgttatc atcacgcatt aaaaacgcga tttgcgtacc ctgcggatta
    28321 cagcgcaccc agtggcgcgg gacgttgact aaccccggat aagcccgatg atgggtaaag
    28381 gttaaacgtc gctgcacgac gccacgcggt ggcgcgggta gggttgtttc cgttccactt
    28441 aacggcgcat cacctgccga tttccagcta gcttcatctt gcggtaactc aacgataaat
    28501 agctccggca ctttctcgcc ctttggcgaa agtgtgtcgc caataaacgc cagcgcgtga
    28561 tttcctaccc atccttcttc ataagcacga ttgatttcat cactgccagg ctgcggcgtg
    28621 ggcgtggttt tactcaccag cacacaccag tggctaccgc tgtattcacg cggatgttgc
    28681 ttttgtacgt tgaccgggcc aaacggtgca gcaaccccga cgtttcgcaa atccagcgcc
    28741 ggatcgagtt gatgcattac atggtcgtta taggtaaagc tccccctttc accgttcggg
    28801 ctaaagacat gcacatggct gccaccgcgc agcgctcctg gcgtatacgg cgcggtaata
    28861 tccattgcat cgagattgct caccttgccg ccttcagcaa tgactccgcg tcgatgatgg
    28921 aagtcgtaat gccatgtttc atcaggattt tcagggccgt gaataaagac atatttctct
    28981 gacttcggat gaacggtcac cacgccgacg tgtgcgccct gtgacgcgcg atagataacc
    29041 tcgacctcgc ctgtatggat attcacacgc tcaatggtct cgccggtaaa cgacgcccca
    29101 gaaggacgca cgtcaaatac cagccactgg ctgtcgggcg tccaggtatt ggtattggta
    29161 ttggtgagca ggtgattacg gggagcaaag gttatctgtt tcatggcgcg gtcctgatac
    29221 aaaacggttc ccgccatcat acttcacaag gacttcactt tcagacaata gtcttttgcc
    29281 atcgtgatga atgtgagtga aaagatggaa catttcgacg tggcgattat tggcctcggc
    29341 ccggcagggt cggcgttggc acgaaagtta gccggcaaaa tgcaggtgat cgcgctggat
    29401 aaaaagcacc agtgcggtac tgaaggtttc agcaaacctt gcggcggtct gctggcaccg
    29461 gacgcgcagc gatcttttat tcgcgatgga ctgacgcttc ctgtcgatgt gatcgccaat
    29521 ccacagattt tcagcgtcaa aactgtcgac gtcgccgcat cgctcacgcg taactaccag
    29581 cgaagctata tcaatattaa tcgccatgct ttcgacttgt ggatgaaatc gctgatcccc
    29641 gccagcgttg aggtttatca cgatagcctg tgccgaaaaa tctggcgtga ggatgataaa
    29701 tggcatgtca tttttcgtgc agacggctgg gagcaacata ttaccgcccg ctatctggtc
    29761 ggtgccgatg gcgcaaactc gatggtgcgg cgacatctct acccggatca tcaaatccgt
    29821 aaatatgtcg ctatccagca gtggttcgca gagaaacatc cggtgccgtt ctactcctgc
    29881 atctttgata atgcgataac tgactgttac tcatggagta tcagcaaaga tggttatttt
    29941 atctttggcg gtgcctatcc aatgaaagac ggtcagacgc gtttcacgac gctgaaagag
    30001 aaaatgagcg cctttcagtt ccagtttggc aaagcggtga aaagtgagaa atgcacggtg
    30061 ttgtttccct cacgctggca ggattttgtc tgtggtaagg acaacgcctt tctgattggt
    30121 gaagcggcgg gatttatcag cgccagctcg ctggagggga ttagctatgc gctggatagc
    30181 gcagagattc tgcgtgcggt gttcctgaaa cagccggaga agagcaacgc cgcttactgg
    30241 cgcgccacac acaaactgcg tttaaaactc ttcggcaaga tagtaaaaag ccgatgcctg
    30301 accgcaccgg ctttaagaaa gtggattatg cgcagtggtg tggcgcatat tccacagttg
    30361 aaagattatc caacgcgctt cacatcaccc accagcagga tgtaagagag cgcgccaatc
    30421 aacgcgacgg cggagatata aaccagtgca ggcgcgaaac cgtaaccctg cgccaggtac
    30481 cccaccacca gcggaacggt gatgccgccc agaccaccgg cgaagttaaa cacgccgccg
    30541 gttaaaccaa tcaggcgcat cggtgccaga gaagagacca gcgaccaggt aatcgaagca
    30601 aaaccgttgc cgaagaatgc cagcgccatc aggcacataa tcatcatcgg atcgttggtg
    30661 tagttagcgc ccataatgca ggtggagatc agcaagccgc agataatcgg cgttttacgc
    30721 gcaaagccca gtgaaaagcc cttacgtacc agcagatccg cgacccagcc agagagcagg
    30781 acgccgacaa acgccgcgag gaatggcacc gtggtcataa agcctgcttt cagcgccgtg
    30841 attccttttt cctgggttaa atagttcggg aaccaggtta agaaaaacca cagtgcagaa
    30901 gccaccgcaa attgcccaag ataaacgccg atcagtttac gatggaacac cagtttccag
    30961 tctttggctg ttaacggctg acgcgcctct ttcttcaccg gcgcatcacc atccaccaga
    31021 ccgccgccat cacgaatgta atccagttca gctttgctga tacctttggt caggcgcggc
    31081 ggctgataaa ccttaaacca aatcagcgac cagataatgc cgataccacc agtgacaatg
    31141 aacacccagt gccagctcaa catctcctga atccagatca gcagcggagt caaaaacgcc
    31201 agaccgacaa actgaccaga cgtataaaaa ccaacggcgg aagcgcgttc atgttccggg
    31261 aaccagctgg tcaccatccg gttattggtc gggaaagcgg gcgcttcgaa aataccggtt
    31321 atcgcacgca gaccaattaa tgacattaat cccgtggcaa agccttggaa taaagtcacc
    31381 actgaccaac caaatatcgc aataaaataa gtcacgcgag aacctacgcg atctaaaaac
    31441 caaccgccgg ggatctggca tagcgtataa agccaggcga aggccgaaaa tacatagccc
    31501 atttccgctt tcgtaatgcc gaactcttcc tgaatatggg cggaagccac ggccaagtta
    31561 gcgcggtcaa cataacaaat gaccaccgta ataaagatca tcaccagcgt cagataacgc
    31621 cgacgccccg gctttgctgc attaacggga atatccatag cgagctttct ccagattttg
    31681 ggcatagcga agccgctcac catgccctgt aatttacaga gggttatatt tttgtattgc
    31741 tgttttagtg cccgatgagg ggcttacgtg gcaggaatta ccactctgct acgctgttat
    31801 cttcatgacg ccagagcgga ttacgccagt ccggggcatt tttactgaac tcaataactt
    31861 tagcttcgtc gatttccaca cctaagcccg gtttcgttaa cggtttaaag aagccgccaa
    31921 ccatgctgaa gtcttctttg tttttcacaa agtcgagtaa ctccgcgcct ttgttgtaat
    31981 gaatgcccat actttgttac tgaagtaccg cgttatagga aacaaagtcg atatgcaggc
    32041 aagccgccag tgcaatcggt ccgaacggac agtgcggcgc aagggtcaca tcataggctt
    32101 ctgccattcc agcaattttg tagcattcgg taataccgcc tgcatgggag agatccggtt
    32161 gcagaatcga aataccgcct gcctccagca cgcgtttaaa atcgaaacgt gagaacatgc
    32221 gctcacccgc cgccagtgga atatgcgttt gtgccgccaa tttcgggtag tattcggctt
    32281 gttccgccag caccggctct tcaataaaca gcgggcgata cggctccagt tctttaatca
    32341 gcactttcgc cattggcgcg ctgacgcgac cgtggaaatc aagaccaaac tcaatctgat
    32401 tgccaaaagc ttcacgaatt tgtgccacgg tgttgactgc cgcatctacc gcgcgggagt
    32461 tatcaattag ccccagttct tcacaaccgt tcagtttgaa ggtatcgaag ccgatttcgc
    32521 gcagggtttt aatgccgtcg ataacatccg ccggacgatc gccgccgacc caactgtagg
    32581 ctttaatttt gtcgcgaacc aggccgccca tcagttgcca gactggcgca ttcagcactt
    32641 tgcctttgat atcccataac gcctggtcga tcccggcgat ggcgctcatc aggattggac
    32701 cgccacgata aaatccggcg cgatacatca cttgccataa gtcgttgatg cgcgaaggat
    32761 cctgaccaat caaatagtca cccagctcgt gaactgccgc ttccaccgta cgggcgcggc
    32821 cttcaatcac gggctcgccc caaccgacca cgccttcatc ggtttcaatt ttcaggaaca
    32881 tccagcgggg aggtaaacga tacgtggtaa ttttggtgat tttcattgca ctgcctctcg
    32941 atacgcctta acaaatgctg ctgcctgctg cgcggtgcgt tctacggact gcccggcgcg
    33001 ataaagatcg ctgcctaagc ccgccccaac acaacctgcg tctatccact gcgccaggtt
    33061 ttctggcgtc acgccgccaa cggcaaagac tgcgatgtcc ggtggtaata ccgcttttaa
    33121 cgctttgatg tattgcggac caaaagccga tgacggaaat attttcagcg cctgcgcgcc
    33181 cgcttcgagc gcggtaaagg cttcggtcgc cgtcgcgcag ccggggcaaa cggtcatgcc
    33241 gtagcccacc gcgcggcgga tcacttcact atggatattg ggcgtaacga tgagctgaca
    33301 gcccatcctg gcgagcgcat cgacctgttc aggtttcagt accgtacctg cgccaatcaa
    33361 cgccttgtcg ccatacgcat caacgatggc gggaatgctc tgctcccatt gtggggaatt
    33421 cagcgggatt tcaaccgcgt cgaacccggc gtcaatcacc gcgccaacat gcgccagcgc
    33481 ctcgtcgggc gtaatgccgc gcaaaatggc gatcagcggg agtttagttt gccactgcat
    33541 gagcgatgct ccttatacca gcctgaaatg ccgtgtcgcc cgccaccgcc gccacgtcgc
    33601 aacccatcgc ctgaaaggct tgctggtagc gcgcggtcag cgatgttccg gcgacaaggg
    33661 tgatggcgtg ttgatgggtc acatagtcgc gcatactggc aacctctgcg ccaatcaaca
    33721 aaccagagag aaattcgctg acctgttcgc ggggaagtgt tcccagcaca tgcgaggcgc
    33781 gaacttcaaa aagctgcggc aatatggcgg gcgcattaag gccgcgctca aggccagccg
    33841 cgaaggcatc ggcagagttt tcctgcggcg gcaaacctgc gccaatcaat gagtgattta
    33901 acagtaaatg atgtaattca ccggtcatca cggtgcgaaa atcgttgatt tgctggctat
    33961 cggcctgcac ccatttgcaa tgcgtaccgg gcataacata aagagaggaa ggagccagag
    34021 ctcgcgcgcc gatcaattgt gtttcttcgc cgcgcatcac attgtggtta tcgtcatgag
    34081 agacgcataa tccgggaata atccagatat tgtcgccaac agacgttaat tgttcgccaa
    34141 tagacgaaaa acgggcagga acagataaat acggagcaac tttccagccg acattgctgc
    34201 cgaccattcc tgccatcacc actggcgtat tttcttcacg ccagtcggtc gtgacttctg
    34261 ctaacaccac agccggagat tttccgttca ggcgcgtgac gcctgcttct gattgcctgc
    34321 tctccaggca gtggtcgccc tgataaagcc aggcgcgcag attggtcgat ccccagtcaa
    34381 ttgcgatgta gcgagctgtc atgtgatttc ctttaacctt cgtgtcgagc tggcaatcat
    34441 ggtaagcgcc gcctgctctg ccgcatcgcc gtcctgatgc cgtatcgcat cgaacagcgc
    34501 cttatgttcc tggagcgttt gcggcatgtt ggcctcatcg cccatccagg ttcgttcaaa
    34561 aaccgcccgc tgcagcgaac tgatcgcaat gctaagttgc tgtaacaccg ggttatgcac
    34621 cgactgcagc accgcctcgt ggtagcgaat atccgcttcg ttaaacgctt cgcggtcctg
    34681 attgttggca atcatctcgt tcagcgccga ttcaatctgc gccagatcgc tggaggtcgc
    34741 gcgctctgct gcccaacggg caatcgccgg ttccaccaga tttcgcactt cgctcatggc
    34801 actgataagc cgtgggtcgt agtcgttttc cagcacccat tgcagtacgt cagtgtcgag
    34861 gtaattccac tggttacgcg gtgccacaaa cgccccgcga taacgtttca tttcaatcag
    34921 ccgcttcgcc atcagcgaac ggaacacctc acggatgatg ttgcgcgagg ttgcaaactc
    34981 ttcacagagc tccgcctcag ccggaagtgg cgagccaggt acgtatttgc cgtgaacgat
    35041 ctgtttaccc agcgtaatga caatgcgatc ggttttattg agagtcatgg agagtccttg
    35101 tgctctgtat gttcttctct actttacccc gatcgatgca taacgcggca actttgtagt
    35161 accagcgtga tgacgttcgc gtttgccgcg cgcgtaatgt agtacaaact tatattgtta
    35221 tgctacaatt tagatcaaaa aaagaacaat acataaaaaa atgacatgcg tcgggcagaa
    35281 atctgaaaag ggatatcagg cgctaaacag gagggaaaga agagtatgct ttcaacggct
    35341 tagctactcg tttaaaggat taatcatgaa gttgaatttt aagggatttt ttaaggctgc
    35401 cggtttattc ccactggcgc tgatgctttc aggctgtatc tcgtatgctc tggtttccca
    35461 taccgcaaag ggtagttcag gaaagtatca atcgcagtca gacaccatca ctgggctatc
    35521 gcaggcaaaa gatagtaatg gaacaaaagg ctatgttttt gtaggggaat cgctggatta
    35581 ccttatcact gatggtgccg atgacatcgt taagatgctc aatgatccag cacttaaccg
    35641 gcacaatatt caggttgccg atgacgcaag atttgtttta aatgcgggga aaaagaaatt
    35701 taccggcaca atatcgcttt actactactg gaataacgaa gaagaaaagg cactggcaac
    35761 gcattatggt tttgcctgtg gtgttcaaca ctgtaccagg tcactggaaa acctaaaagg
    35821 cacaatccat gagaaaaata aaaacatgga ttactcaaag gtgatggcgt tctaccatcc
    35881 atttaaagtg cgattttatg aatactattc acccagaggc attccggatg gtgtttccgc
    35941 agcattactg ccagtgactg ttacactgga catcattact gcaccgctgc aatttctggt
    36001 tgtatatgca gtaaaccaat aatcagtaag cgggcaaacg cgtttatgct gtttgcccgc
    36061 ttacagatta attcagcaca tacttctcaa tagcaaacgc cacgccatct tcaaggttgg
    36121 attttgtgac aaagttcgcc acttctttca ccgaaggaat agcgttatcc atcgccacac
    36181 cgacgcctgc atattcaatc attgcgatgt cgttttcctg atcgccaatc gccatgattt
    36241 cttccggttt aatacctaac acgtcggcca gtgatttcac acccgtacct ttgttaacgc
    36301 gtttatcgag gatttcgagg aagtacggcg cacttttcag cacggtatat ttctctttca
    36361 cttcctgcgg aatacgcgcg atagcctggt cgaggatggc gggttcatca atcatcatca
    36421 ctttcaggaa ctgggtattg gggtccattt tctccgcttc gcagaacacc agcggaattg
    36481 tggcaacgaa ggattcatgc accgtgtagt agctgatatc gcggttagcg gtgtacagcg
    36541 tggtgcggtc cagggcgtgg aaatgagaac cgacttcgcg agagagtttt tccaggaaac
    36601 gatagtcgtc atagctgaga gcggtttgcg ccacggtgct accatcagcg gccttctgta
    36661 ccagcgcgcc gttataagta atgcagtagt cgcccggctg ttccatatgc agctctttca
    36721 ggtagttgtg cacacctgca tacggacgac ccgtcgttag cacgacattc acgccacggg
    36781 cgcgagctgc ggcaatcgca tttttaacgg cgggtgaaat ggtgtgatcg ggcagcagaa
    36841 gggtgccatc catatcgata gcaatgagtt tgatagccat gagttcccaa ggtagattag
    36901 ttcctgaccc atgctaacgc gattccgctc aaaaatcagt acaacacccg agggaaaagg
    36961 ggatgcaacg cgggttgcat gctccccatt tgcttagcgg aagagtttcc ctttcagcag
    37021 ttccatgcct gcggaaagca gatcgttatt ggcttgtgct gacacttcac cttgcggtga
    37081 gagcgcatca ataatcttcg gcaattgctc tgccagtaaa ctggaagccg tactggtatc
    37141 cacgccaagt ttttgcccga gatcggacac cgcatttgtg ccgagcgccg actccagttg
    37201 ctcgccgcta actgattgat ttctctgttg attactcaac caggttgaga gaatggcccc
    37261 taagccgcca ctttgcagtt tttccagcag cacctgaatg ccaccctgct cctcaaccca
    37321 acttaaaatc gcctgatatt tccccgcatc gcctttcaga aaggcaccga caacttcatc
    37381 aaaaagcccc atgataatca cctgtaaagc gttacgtgtt gacccaaaaa gtatagatct
    37441 gcgggatgat aattgcggat tgcagaaata aaaagggcgg agatgatctc cgcccttttc
    37501 ttatagcttc ttgccggatg cggcgtgaac gccttatccg gcctacaaaa tcatgcaaaa
    37561 tcaatatatt gcaggagtct cgtaggacta ataagcgtag cgcatcaggc acgctcgcat
    37621 ggttaacgcc attaaatatc aatattcgcc gctttcaggg cgttctcttc aataaacgca
    37681 cgacgcggtt ctacggcgtc gcccatcagc gtggtgaaca actggtcagc ggcaatcgca
    37741 tccttaacgg taacgcgcag catacgacgg ctttccgggt ccatggtggt ttcccacagc
    37801 tgttccgggt tcatctcgcc cagaccttta taacgctgga tggagaggcc acgacgggac
    37861 tctttcacca gccagtccag cgcctgctcg aagctggcta ccggctgacg acgctcgcca
    37921 cgttcgataa acgcatcttc ttccagcaag ccacgcagtt tctcacccag cgtgcagata
    37981 cgacgatatt cgccaccggt gataaactcg tgatccagcg gatagtcagt atccacaccg
    38041 tgggtacgca cgcgaacaat cggctcgaac aggttttgtt cggcattggt gtggacatca
    38101 aacttccact ggctgccgtg ctgttctttg tcgttcagtt cgctgaccag cgcgttcacc
    38161 cagcgggtaa cggtctgctc atcagaaagg tcagcttccg tcaacgtcgg ctgatagata
    38221 agctctttca gcattgcttt cggataacga cgctccatgc ggttgatcat tttctgcgtc
    38281 gcgttgtact cagataccag tttctctaac gcttcgccag ccaatgccgg tgcactggcg
    38341 ttggtgtgca gcgttgcgcc gtccagcgcg atagagatct ggtactgatc catcgcttcg
    38401 tcgtctttaa tgtactgttc ctgcttgcct ttcttcactt tgtacagcgg cggctgagcg
    38461 atgtagacgt gaccgcgttc aacgatttcc ggcatctgac gatagaagaa ggtcaacagc
    38521 agcgtacgaa tgtgcgagcc gtcgacgtcc gcatcggtca tgatgatgat gctgtgataa
    38581 cgcagtttgt ccgggttgta ctcgtcacga ccgataccac agccaagcgc ggtgataagc
    38641 gtcgccactt cctgagaaga gagcatctta tcgaagcgcg ctttctcgac gttaaggatt
    38701 ttacccttca gcggcagaat cgcctggttc ttgcggttac gcccctgctt cgcagagccg
    38761 cccgcggagt ccccttccac caggtacagt tcggaaagcg ccggatcgcg ttcctggcag
    38821 tctgccagtt tgcccggcag gccagctaag tcgagcgcac ctttacggcg ggtcatttca
    38881 cgcgcgcgac gcgccgcttc acgggcacgg gcagcatcga taattttgcc aaccacgatt
    38941 ttcgcatcag ttgggttttc cagcaggtat tccgccagca gttcgttcat ctgctgttca
    39001 accgccgatt tcacctcaga agaaaccagt ttgtctttgg tctgggagga gaatttcggg
    39061 tccggcactt ttacggaaac gaccgcaatc aggccttcac gcgcatcgtc accggtggcg
    39121 ctgactttgg cttttttgct gtagccttct ttgtccatgt aggcattcag ggtacgggtc
    39181 atcgccgcac ggaagcctgc caggtgagta ccgccgtcac gctgcggaat gttgttggta
    39241 aagcagtaga tgttttcctg gaagccatcg ttccactgca gcgccacttc aacgccaata
    39301 ccgtcttttt cggtggagaa gtagaagata tttgggtgga tcggcgtttt gttcttgttc
    39361 agatattcaa cgaacgcttt gatgccgcct tcatagtgga agtggtcttc tttgccgtca
    39421 cgcttgtcgc gcagacgaat ggaaacgccg gagttgagga acgacaactc acgcagacgt
    39481 ttcgccagaa tttcatattc gaactcggtc acattggtga aggtttcgag gctgggccag
    39541 aaacgcacca tggtgccggt tttttcagtc tcgccggtaa ccgccagcgg agcctgcggt
    39601 acaccgtgtt cgtagatctg acggtgaatt ttaccctcgc gctgaataac cagctccagt
    39661 ttttgcgaca gggcgtttac taccgaaaca ccaacgccgt gcagaccgcc ggacacttta
    39721 taggagttat cgtcaaattt accgcctgcg tgcagaacgg tcatgatcac ttccgccgcc
    39781 gatacgccct cttccgggtg aataccggtc ggaatgccgc gcccgtcatc ctgtacagag
    39841 acagagttat cggcgtgaat ggtgacgata atttctttac agtgacccgc gagcgcttcg
    39901 tcgatagcgt tatctaccac ctcgaatacc atgtggtgca gaccggtgcc gtcatccgtg
    39961 tcgccgatat acatacccgg gcgcttacgc accgcatcca gccctttcag gactttgata
    40021 ctggaggagt cataagaatt cgacatcaac gtttctcgct catttatact tgggttaatc
    40081 tgttatttta cccttttcca cggtaaacat cttcgaattt tcgtccgaca tgtctataac
    40141 gtgttcagca ctgatcgcgc tgacaaagac ctgtgattgc gtcgctttta agcggctggc
    40201 aagcaacccg cgacgctcat catcaagctc agaggcaaaa tcatctatca ggtagagaca
    40261 ccgccgcccg ctttcacgag tgaggaactc tccttgcgcc agacgtaagg cgcacatcaa
    40321 cagcttaagc tgcccacgcg ataaggtatc ttccaccggc gcaccgtcgg cgcgaatgcg
    40381 taagtccgct ttatgcgggc catgcgcggt gtaggttagc tggcgatcgc gttcaaaatt
    40441 acgttccagc acctcagcgt actctgtctc tttctcccag ccgcgctgga aagagaaagt
    40501 cagagaaaac tcagggagaa attgcttaca ggtatcatcc atatcggccg cgataccggc
    40561 gctatactcc gcgcgccagg tgctgatttg ctccgccagc gggatcagtt ctttatccca
    40621 cgggcgtagc tgttcgtaac gagtcacctg gcgcagcgcc gcattgcgct gcttgagcaa
    40681 tcgcttgaga ttgctccagg cggtgaaaaa tccaggttcg ttgtgaaagc atccccagtc
    40741 gaggaatgct cttctgtatt tggggccgcc gttgagtaaa gtaaaccctt ctggcgttat
    40801 caactgcatc ggcatcaggt gcgccagttc cgcgacttta tgcccgtccg taccgtcaat
    40861 acggactttg ctgtcgccct gtttgtcttt ggttaagcca atcgccgtct cgcgctcttc
    40921 gccctgtaat cgcccgtgga gaacaaatgc ctcctgctca tggcgaatga cgcgaccaat
    40981 ctgcaaactg cgaaacgccc gaccatggcc gagcgtatag atggcttcca gcacgctggt
    41041 tttgccactg ccgttggcac ctaccagaaa gttaaagccg ggagataaag cgagatccgc
    41101 ggtttcaata ttgcggaaat cgcggatcaa caagcgggtg agggacatta cagtctcatt
    41161 ggcatgacaa cataggcagc cgactgtgat gcggcatctt caatctgcac gctggaaacc
    41221 gaatcggtca gcatcatgcg gacgttttcg catttcagcg cgttcagaac atccagcaca
    41281 tagctgacgt tgaagccgat ttccatctcc gcaccgctat aggtaacgtc gaggatctct
    41341 tccgcttctt cctgttccgg gttgttggca gtaattttca gctggttttc gctgacatac
    41401 aggcgcacac cgcggaattt ctcgttagag agaatcgccg cgcgagcaaa cgcctgcttg
    41461 agcagatcgc agcctgcttc cagatgtttg tccgggttct tcggcagaac gcgacggtaa
    41521 tccgggaagc gaccatccac cagtttcgag gtgaaaataa agtcgccaac gtgggcacga
    41581 atgttattgc tgccaatctg cacacgcagc agattgtcgc cgccgtcgag catacgcatc
    41641 agttcaatca cgcctttacg cggtacgatc accgaatggc ttggcaaaga ttgaccaatt
    41701 ggcattgaac agaccgccag acggtggccg tcggttgcca cggtgcgcag ttcttcacct
    41761 tcggtttcaa acagcatacc gtttaagtaa tagcgaacat cctgatgcgc catcgaaaac
    41821 tgggttgctt caatcagacg cttcatcgtc gcctgcggca gggtaaattc gacttcgctc
    41881 tgccagtcat cgaggttcgg gaaatccgcc gctggcaggg tagacagcga aaaacggcta
    41941 cgcccggagc gcaccagcat ccgttcacct tccagctgca cggcaatttc cgcgccttca
    42001 ggcagaccac ggcagatatc aaagaatttg cgcgccggaa cggtcgtcgc tcctggctcg
    42061 tgtggctgaa ccagcgcaac gcgtgccacc atttccatct cgagatcagt accggtcagc
    42121 gacaacgtac cgtcagcaac ctgtaacagc agattaccga gaatcggtag cgtaggacga
    42181 ccacctaacg gaccgctcac ctgttgtagc ggttttaata aatgctcacg ttctacggta
    42241 aatttcatag gtttacgatg acaatgttct gattaaattt gaaaaatctt ctttgatatc
    42301 gtggctctct tcacgcaact gctcgatctt acggcaggca tgaagcaccg tcgtgtggtc
    42361 acgcccacca aacgcatcgc caatctctgg cagactgtgg ttagtcagct ctttcgctag
    42421 cgccatcgcc atctggcgcg gacgcgccac cgagcgggat cgacgcttgg aaaggagatc
    42481 cgcgactttg atcttgtagt actccgccac cgtcttctga atattgtcga tggtgaccag
    42541 tttttcctgc aatgccagca agtcgcgcag cgcctcacgc acgaagtcga tggtgatcgc
    42601 ccgtccggta aagttggcgt tagcaataac gcggttcagc gccccttcca gctcacgtac
    42661 gttagatcgt agacgcttgg cgataaagaa cgccacttca cccggcaaac gaatgtcatt
    42721 ttcgtcggcc tttttcatca ggatcgccac acgggtttcc agctctggcg gttcgatcgc
    42781 cacagtcagt ccccaaccga agcgggattt caaacgatcc tcaacgccgt tgatctcttt
    42841 cggatagcga tccgaggtga gaatgatctg ttgattacct tccagcaggg cgttgaaggt
    42901 gtggaaaaac tcttcctgag atcgttcttt attagcaaaa aactgaatat cgtcgatcag
    42961 cagtgcatct acggaacggt agtagcgttt aaactcttcg atcgcgttgt tttgcagggc
    43021 tttaaccatg tcctgaacaa agcgctcgga gtgcatataa accactttgg cattcggctt
    43081 gcgcgccata atgccgttac ccaccgcatg cagcaggtga gttttaccca gacccgtgcc
    43141 gccataaagg aacaacgggt tataggcacc gccagggtta tccgccacct ggcaagccgc
    43201 cgcgcgcgcc agttggttag atttaccttc aacgaagtta tcaaacgtgt gtttgacgtt
    43261 tacgttagaa cgataggtcg gttctgccgg ggccgggacg ttatcccaac ctgagcgcgt
    43321 agaaggcgca gcacgttgcg gctgcgtttg cgccacctgt gcaggggccg cgacgttgct
    43381 cgtcactgcc gcttgtggcg tttgcgtcac cggtttggtg ccgacttcaa aacgcagctg
    43441 tggggcatcc gctccgcaga aactggttag cagtccattg atattattaa ggtacttgtc
    43501 ccgtacccaa tcgaggacaa aacggtttgg cgcgtacagg gccagcgtgt tatcgctcag
    43561 ttccgcctgc aatgggcgta tccacatact gaattctgtg gctggtaact catcctgcaa
    43621 tcgggcaaga cactgctgcc aaagcgaaag tgacacggcg gactccactc gaacaaaagt
    43681 cgataatgac taaggctgaa acattcatga ttgttgacgt acgtcgaaaa gaccctgctt
    43741 gagggtgacg cacgaaccgc tgtctgcggt tatatgcccg atcaagatcc tgcaaaacga
    43801 tcgggaccgc ggatcatagc ctaaactgcg caagagatct tctgtttctc acagattttc
    43861 cctatttatc cacaggactt tccagaactc gctaagtgta aacgatcctg ccgcgaggcg
    43921 ggcacgattt acgccgcata ttggaaaatt taatgaccat agacaaaaat tggcttaatc
    43981 gatctaataa agatccagga cgatccttgc gctttaccca tcagcccgta taatcctcca
    44041 cccggcgcgc catgctggtt tccactggtg tgaggtcgta cattttccct gcgaaaaggt
    44101 gcggaaaagc gcggtaaata aggaaagaga attgactccg gtgtgtacaa ttattacaat
    44161 ccggcctctt taatcaccca tggcttcggt gtccatcgtt tcatttttcg gcggatatcc
    44221 aataaagcca ttgaatttat tcaagtttag gtagaaatcg ccatgaaacg cacttttcaa
    44281 ccgtctgtac tgaagcgcaa ccgttctcac ggcttccgtg ctcgtatggc tactaaaaat
    44341 ggtcgtcagg ttctggcacg tcgtcgtgct aaaggccgcg ctcgtctgac cgtttctaag
    44401 taataaagct aacccctgag tggttaagct cgcatttccc agggagttac gcttgttaac
    44461 tcccagtcaa ttcacattcg tcttccagca gccacaacgg gctggcacgc cgcaaattac
    44521 cattctcggc cgcctgaatt cgctggggca tccccgtatc ggtcttacag tcgccaagaa
    44581 aaacgttcga cgcgcccatg aacgcaatcg gattaaacgc ctgacgcgtg aaagcttccg
    44641 tctgcgccaa catgaactcc cggctatgga tttcgtggtg gtggcgaaaa aaggggttgc
    44701 cgacctcgat aaccgtgctc tctcggaagc gttggaaaaa ttatggcgcc gccactgtcg
    44761 cctggctcgc gggtcctgat agccctcatt cgggtctatc aacgcctgat tagtccgcta
    44821 ctcgggccgc attgtcgttt cactccaacc tgttcaagct acggaattga ggcattgcgc
    44881 aggtttggag tgataaaagg cagttggttg acggtgaaac gcgtattaaa atgccaccct
    44941 ttacaccctg gtggtgacga tcccgtcccg cccggaccat ttgataccag agaacactaa
    45001 cgatggattc gcaacgcaat cttttagtca tcgctttgct gttcgtgtct ttcatgatct
    45061 ggcaagcctg ggagcaggat aaaaacccgc aacctcaggc ccaacagacc acgcagacaa
    45121 cgaccaccgc agcgggtagc gccgccgacc agggcgtacc ggccagtggc caggggaaac
    45181 tgatctcggt taagaccgac gtgcttgatc tgaccatcaa cacccgtggt ggtgatgttg
    45241 agcaagctct gctgcctgct tacccgaaag agctgaactc tacccagccg ttccagctgt
    45301 tggaaacttc accgcagttt atttatcagg cacagagcgg tctgaccggt cgtgatggcc
    45361 cggataaccc ggctaacggc ccgcgtccgc tgtataacgt tgaaaaagac gcttatgtgc
    45421 tggctgaagg tcaaaacgaa ctgcaggtgc cgatgacgta taccgacgcg gcaggcaaca
    45481 cgtttaccaa aacgtttgtc ctgaaacgtg gtgattacgc tgtcaacgtc aactacaacg
    45541 tgcagaacgc tggcgagaaa ccgctggaaa tctcctcgtt tggtcagttg aagcaatcca
    45601 tcactctgcc accgcatctc gataccggaa gcagcaactt cgcactgcac accttccgtg
    45661 gcgcggcgta ctccacgcct gacgagaagt atgagaaata caagttcgat accattgccg
    45721 ataacgaaaa cctgaacatc tcttcgaaag gtggttgggt ggcgatgctg caacagtatt
    45781 tcgcgacggc gtggatcccg cataacgacg gtaccaacaa cttctatacc gctaatctgg
    45841 gtaacggcat cgtcgctatc ggctataaat ctcagccggt actggttcag cctggtcaga
    45901 ctggcgcgat gaacagcacc ctgtgggttg gcccggaaat ccaggacaaa atggcagctg
    45961 ttgctccgca cctggatctg accgttgatt acggttggtt gtggttcatc tctcagccgc
    46021 tgttcaaact gctgaaatgg atccatagct ttgtgggtaa ctggggcttc tccattatca
    46081 tcatcacctt tatcgttcgt ggcatcatgt acccgctgac caaagcgcag tacacctcca
    46141 tggcgaagat gcgtatgctg cagccgaaga ttcaggcaat gcgtgagcgt ctgggcgatg
    46201 acaaacagcg tatcagtcag gaaatgatgg cgctgtacaa agctgagaag gttaacccgc
    46261 tgggcggctg cttcccgctg ctgatccaga tgccaatctt cctggcgttg tactacatgc
    46321 tgatgggttc cgttgaactg cgtcaggcac cgtttgcact gtggatccac gacctgtcgg
    46381 cacaggaccc gtactacatc ctgccgatcc tgatgggcgt aacgatgttc ttcattcaga
    46441 agatgtcgcc gaccactgtg accgacccga tgcagcagaa gatcatgacc tttatgccgg
    46501 tcatcttcac cgtgttcttc ctgtggttcc cgtcaggtct ggtactgtac tatatcgtca
    46561 gcaacctggt aaccattatt cagcagcagc tgatttaccg tggtctggaa aaacgtggcc
    46621 tgcatagccg cgagaagaaa aaatcctgat tcggtgagtt ttcgctaaaa taagggcggt
    46681 cagttgaccg ccttttttct tttcgtaggg cggataagca ccgcgcatcc gccacacaaa
    46741 gcaacaggaa catcatgagc gataatgaca ctatcgtagc ccaggccacg cctccgggac
    46801 gtggcggcgt tggcatcctg cgcatctccg gcttcaaagc ccgtgaagtt gccgaaaccg
    46861 tgctgggtaa actgcctaag ccgcgctacg ccgattatct tccgtttaaa gacgccgacg
    46921 gcagcgtgct cgatcagggg attgcgctat ggttccctgg cccgaactcg ttcaccggcg
    46981 aagatgtgct ggaactgcaa ggtcatggcg gtccggtgat cctcgacctg ctgttaaaac
    47041 gcattctgac cattcccggc ctgcggattg ctcgccctgg tgagttttcc gaacgcgcgt
    47101 ttcttaacga taaacttgac ttagcccagg ccgaggcgat tgccgatctt atcgacgcca
    47161 gttcggaaca ggcggcccgt tcggcactta actcgctgca aggcgcattc tccgcacggg
    47221 ttaatcatct ggtagaagcc ctcacccact tgcgcattta cgtcgaagcg gcaattgatt
    47281 tccctgatga agagatcgat ttcctctccg acggcaaaat tgaagcccag cttaatgacg
    47341 tgatggccga tctcgatgca gtgcgtgctg aagcacgtca gggtagtttg ttgcgcgaag
    47401 ggatgaaagt agtgattgcc ggacgtccta atgccggtaa atcgagcctg ttaaacgcgc
    47461 tggcgggccg tgaagcggca atcgtaaccg atatcgccgg aaccacgcgt gacgtgctgc
    47521 gtgagcatat ccacattgac ggaatgccgc tgcatatcat cgacactgcc gggctacgtg
    47581 aggccagtga cgaagtggaa cgtattggta tcgagcgcgc gtggcaggaa attgaacagg
    47641 ccgaccgcgt gctgtttatg gtcgatggca ccacgacaga cgccgttgat ccggcagaga
    47701 tctggccgga atttatcgcc cgtctgcccg cgaaactgcc gatcaccgtg gtacgcaata
    47761 aagccgatat caccggcgaa acgctgggaa tgagtgaagt gaacggtcac gcgttaattc
    47821 gtctctcggc aaggactggt gaaggcgtgg acgtgctgcg taaccatctc aaacagagca
    47881 tgggctttga caccaatatg gaaggcggct tcctggcgcg ccgtcgccac ttacaggcgc
    47941 tggaacaggc agcggagcat ttgcagcaag gtaaagcgca actgttggga gcctgggcag
    48001 gtgaactgct ggcggaagag ttgcgcctgg cacagcagaa cttaagcgaa atcaccgggg
    48061 aatttacttc agacgacctg ctggggcgg
//
LOCUS       DAJTQY010000022        47405 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.022, whole genome shotgun sequence.
ACCESSION   DAJTQY010000022 DAJTQY010000000
VERSION     DAJTQY010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47405
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.022"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            <1..480
                     /gene="sseA"
                     /locus_tag="OO349_001281"
     CDS             <1..480
                     /gene="sseA"
                     /locus_tag="OO349_001281"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HCR5866423.1"
                     /translation="LAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDVLLASHE
                     NTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDELDAIFF
                     GRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLPVEPVK"
     gene            complement(1301..2077)
                     /gene="sseB"
                     /locus_tag="OO349_001282"
     CDS             complement(1301..2077)
                     /gene="sseB"
                     /locus_tag="OO349_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HCR5866424.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(2219..3502)
                     /gene="pepB"
                     /locus_tag="OO349_001283"
     CDS             complement(2219..3502)
                     /gene="pepB"
                     /locus_tag="OO349_001283"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HCR5866425.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKGPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAIMRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(3561..3761)
                     /gene="iscX"
                     /locus_tag="OO349_001284"
     CDS             complement(3561..3761)
                     /gene="iscX"
                     /locus_tag="OO349_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HCR5866426.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(3773..4108)
                     /gene="fdx"
                     /locus_tag="OO349_001285"
     CDS             complement(3773..4108)
                     /gene="fdx"
                     /locus_tag="OO349_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HCR5866427.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(4110..5960)
                     /gene="hscA"
                     /locus_tag="OO349_001286"
     CDS             complement(4110..5960)
                     /gene="hscA"
                     /locus_tag="OO349_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HCR5866428.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQADIPDRSDNRVQRELLDATIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDNAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(5977..6492)
                     /gene="hscB"
                     /locus_tag="OO349_001287"
     CDS             complement(5977..6492)
                     /gene="hscB"
                     /locus_tag="OO349_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HCR5866429.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSLAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(6588..6911)
                     /gene="iscA"
                     /locus_tag="OO349_001288"
     CDS             complement(6588..6911)
                     /gene="iscA"
                     /locus_tag="OO349_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HCR5866430.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(6928..7314)
                     /gene="iscU"
                     /locus_tag="OO349_001289"
     CDS             complement(6928..7314)
                     /gene="iscU"
                     /locus_tag="OO349_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HCR5866431.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(7342..8556)
                     /gene="iscS"
                     /locus_tag="OO349_001290"
     CDS             complement(7342..8556)
                     /gene="iscS"
                     /locus_tag="OO349_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCR5866432.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(8668..9156)
                     /gene="iscR"
                     /locus_tag="OO349_001291"
     CDS             complement(8668..9156)
                     /gene="iscR"
                     /locus_tag="OO349_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HCR5866433.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(9426..10166)
                     /gene="trmJ"
                     /locus_tag="OO349_001292"
     CDS             complement(9426..10166)
                     /gene="trmJ"
                     /locus_tag="OO349_001292"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HCR5866434.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            10285..11088
                     /gene="suhB"
                     /locus_tag="OO349_001293"
     CDS             10285..11088
                     /gene="suhB"
                     /locus_tag="OO349_001293"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HCR5866435.1"
                     /translation="MHPMLNIAVRAARKAGNLVAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAILANMRDELSDALKH"
     gene            11233..12087
                     /locus_tag="OO349_001294"
     CDS             11233..12087
                     /locus_tag="OO349_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR5866436.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQQLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGNVYREQMVDFI
                     LSALNPQN"
     gene            12278..13558
                     /gene="csiE"
                     /locus_tag="OO349_001295"
     CDS             12278..13558
                     /gene="csiE"
                     /locus_tag="OO349_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HCR5866437.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQHVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYIHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(13550..14689)
                     /locus_tag="OO349_001296"
     CDS             complement(13550..14689)
                     /locus_tag="OO349_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HCR5866438.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLPLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     VLLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQVSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(14849..15739)
                     /gene="hcaR"
                     /locus_tag="OO349_001297"
     CDS             complement(14849..15739)
                     /gene="hcaR"
                     /locus_tag="OO349_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HCR5866439.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KLALRDFIAIVQERLASVTA"
     gene            15875..17236
                     /locus_tag="OO349_001298"
     CDS             15875..17236
                     /locus_tag="OO349_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HCR5866440.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLHDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            17233..17751
                     /locus_tag="OO349_001299"
     CDS             17233..17751
                     /locus_tag="OO349_001299"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HCR5866441.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGIAWAEEPPSRTRHLISNC
                     QVSETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            17751..18071
                     /locus_tag="OO349_001300"
     CDS             17751..18071
                     /locus_tag="OO349_001300"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HCR5866442.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            18068..18880
                     /gene="hcaB"
                     /locus_tag="OO349_001301"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             18068..18880
                     /gene="hcaB"
                     /locus_tag="OO349_001301"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HCR5866443.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTTSK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            18890..20092
                     /locus_tag="OO349_001302"
     CDS             18890..20092
                     /locus_tag="OO349_001302"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HCR5866444.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLHHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDAKLLID
                     ENIALKSL"
     gene            20189..20611
                     /locus_tag="OO349_001303"
     CDS             20189..20611
                     /locus_tag="OO349_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCR5866445.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(20659..21531)
                     /locus_tag="OO349_001304"
     CDS             complement(20659..21531)
                     /locus_tag="OO349_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HCR5866446.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(21543..22637)
                     /locus_tag="OO349_001305"
     CDS             complement(21543..22637)
                     /locus_tag="OO349_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR5866447.1"
                     /translation="MKINRIVNEGFMKTMLAAYLLGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGGMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYVLMASGKCGKVVINFPD"
     gene            complement(22670..23668)
                     /locus_tag="OO349_001306"
     CDS             complement(22670..23668)
                     /locus_tag="OO349_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5866448.1"
                     /translation="MYASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(23693..25204)
                     /locus_tag="OO349_001307"
     CDS             complement(23693..25204)
                     /locus_tag="OO349_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCR5866449.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASY
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKIMRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISTNGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            complement(25227..26210)
                     /locus_tag="OO349_001308"
     CDS             complement(25227..26210)
                     /locus_tag="OO349_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCR5866450.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            complement(26307..29408)
                     /locus_tag="OO349_001309"
     CDS             complement(26307..29408)
                     /locus_tag="OO349_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HCR5866451.1"
                     /translation="MWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPALV
                     GMLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMTGF
                     TLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKRAV
                     SAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLHVA
                     NHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEEKR
                     FEQYFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLNGYRLAIREIGKCNAL
                     LDDAVALTPATAIQGVLHGINPDRLTIELSDADGNIVLSYQEHQPQELPLPDVAKAPL
                     AAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNRAD
                     FPQAVAYASQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGNSK
                     AGGYYGLARLAARNDNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARLQR
                     EKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTLAA
                     EMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESIEE
                     CWFARHLLACFYYNKRSYNKAIALWQRCVEMSPEFADGWRGLAIHAWNKKHDYELAAR
                     YLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNLEIALKRDDMTAELLNLW
                     HLTGQADKAADILATRKFYPWEGGEGKVTSQFILNQLLRAWQHLDAREPQQASELLHA
                     ALYYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEAMRCLRLAATGDRTINIHSYY
                     NDQPVDYLFWQGMALRLLGEQQTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLLSL
                     YGDLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAINPAWPKAALFTTVMPFIFN
                     YVH"
     gene            complement(29415..29588)
                     /locus_tag="OO349_001310"
     CDS             complement(29415..29588)
                     /locus_tag="OO349_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5866452.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKS"
     gene            29706..30899
                     /locus_tag="OO349_001311"
     CDS             29706..30899
                     /locus_tag="OO349_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR5866453.1"
                     /translation="MRACINNQQILHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDVPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVLVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGSLETVASLSALKKQARVWLK
                     SQPVNTQLEPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(31097..32350)
                     /gene="glyA"
                     /locus_tag="OO349_001312"
     CDS             complement(31097..32350)
                     /gene="glyA"
                     /locus_tag="OO349_001312"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HCR5866454.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            32677..33867
                     /gene="hmpA"
                     /locus_tag="OO349_001313"
     CDS             32677..33867
                     /gene="hmpA"
                     /locus_tag="OO349_001313"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HCR5866455.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISTGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGEVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(33912..34250)
                     /gene="glnB"
                     /locus_tag="OO349_001314"
     CDS             complement(33912..34250)
                     /gene="glnB"
                     /locus_tag="OO349_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HCR5866456.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(34311..35645)
                     /gene="glrR"
                     /locus_tag="OO349_001315"
     CDS             complement(34311..35645)
                     /gene="glrR"
                     /locus_tag="OO349_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HCR5866457.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLSKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNHFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(35635..36348)
                     /gene="qseG"
                     /locus_tag="OO349_001316"
     CDS             complement(35635..36348)
                     /gene="qseG"
                     /locus_tag="OO349_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HCR5866458.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(36513..37940)
                     /gene="qseE"
                     /locus_tag="OO349_001317"
     CDS             complement(36513..37940)
                     /gene="qseE"
                     /locus_tag="OO349_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HCR5866459.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFCGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSCNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFDPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(38516..42403)
                     /gene="purL"
                     /locus_tag="OO349_001318"
                     /gene_synonym="purI"
     CDS             complement(38516..42403)
                     /gene="purL"
                     /locus_tag="OO349_001318"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HCR5866460.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     ASLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFDLDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFRMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYNFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLCTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVVQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFQRGGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLSLGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            42662..44218
                     /gene="mltF"
                     /locus_tag="OO349_001319"
     CDS             42662..44218
                     /gene="mltF"
                     /locus_tag="OO349_001319"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HCR5866461.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(44215..44751)
                     /gene="tadA"
                     /locus_tag="OO349_001320"
     CDS             complement(44215..44751)
                     /gene="tadA"
                     /locus_tag="OO349_001320"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HCR5866462.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAVGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQSSTD"
     gene            complement(44776..45411)
                     /gene="yfhb"
                     /locus_tag="OO349_001321"
     CDS             complement(44776..45411)
                     /gene="yfhb"
                     /locus_tag="OO349_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HCR5866463.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            45620..46468
                     /locus_tag="OO349_001322"
     CDS             45620..46468
                     /locus_tag="OO349_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCR5866464.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAALRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            complement(46807..47361)
                     /gene="pinE"
                     /locus_tag="OO349_001323"
                     /pseudo
     CDS             complement(46807..47361)
                     /gene="pinE"
                     /locus_tag="OO349_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364430.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA recombinase"
BASE COUNT        11309 a        12979 c        11917 g        11200 t
ORIGIN      
        1 cttgcaggct ggcagcgcga tgacctgcta ttagaagaag gtgcagtaga gctgccggaa
       61 ggagagttta acgccgcgtt taatcctgaa gcagtggtga aagtaaccga tgtgttattg
      121 gcaagccatg aaaatacggc gcaaattatt gatgcccgcc cggctgcacg ttttaacgca
      181 gaagttgatg aacctcgccc aggtttacgt cgcggacata ttcccggtgc attgaacgtt
      241 ccgtggacgg aactggtgcg cgaaggcgaa ctaaaaacga ccgatgaact ggatgcgata
      301 ttttttggtc gcggcgtcag ctatgacaaa ccgattatcg tcagctgcgg ctctggtgta
      361 acggcagccg tggttttgtt agcactcgcg acgctggatg tgccaaacgt gaaactgtac
      421 gacggcgcat ggagtgaatg gggcgcgcgg gcagatttac cggttgagcc agtgaaataa
      481 gtattttaca ggcaataaaa aaccgccgaa tttggcgttt ttttattgct ggtctggttc
      541 gcggcctttc cagcaggttg acttgtgtta cacatgagca acgcaggtgc ttcacagcaa
      601 aacaatactc accagtaact ctctttttgt caagcaaaag agagtaatta ttgtttattt
      661 agcgtattat cgacaccggc cctttccgcc gtgttcggta ataaaataac ctggcttatt
      721 agtccgaatt cagacaaata taaacaaatc ctgctcaaaa ttaaaaattc taaccggtaa
      781 aagatattac ttaaacatgt aaatttactt tcctttaaaa aacaaaaaac cgccaaaatc
      841 aggcggttat ttgttgctgg tccggttcgc ggcctttcca gcaggttgta ttaccgtagt
      901 aatgcaagcg cgtctcagcg gagacaatac tcgccagtaa ctctcttttt gtcaagcaaa
      961 agagagttat tattgttctg ttagtgtatt atccactgcg gccctttccg ccgtctcgca
     1021 agcgggcgct ggcttaagga aaggatgttc cgcgaccgtt agagcaagcg cgtctcagcg
     1081 cttgctatcg cggcaatatc gccagtggtg ctgtcgtgat gcggtcttcg catggaccgc
     1141 acaatgaaga tacggtgctt ctgtatcgta cttattgttt ctggtgcgct gttttcccaa
     1201 gtaaataata accggagtct ctccggcgac aatttactgg tggttaacaa ccttcagagc
     1261 agcaagtaag cccgaatgcc gccctttagg gcggcatatt ttagattatc cggttttgtt
     1321 taaagtcacg caaaaaacca ccccagcgac gttcatagaa tggcgcaata tgttcggtaa
     1381 taaagtggct aattcctttt tccccttttt tcacctgaca aatatcgatt ggttcatcgc
     1441 caggtaatgt atcggtcgct acacttcccg tcgcctgaat aatttcttcg atatcaccat
     1501 cggcttcaat gccaataagt aaattaggct gtgcctcttc gttctcttta attgaacaaa
     1561 taaaagcacg cttcaccggc ttaatggttt taaataaggt ggtgagtgaa tcaatcattt
     1621 gtgctggcgg ctctgcgact tccgataata tcagcgattc accgccttcc aggatttcct
     1681 ggctgctcag cggatttccc tcttcaccaa tcaacaaact gatttcacgc ggcataaatt
     1741 ctttaccggt cggcagtttg gcattaagga agagcgtttc gccaagggtc atctcaaaca
     1801 atgtgcgaac gggcattacg acaaatgcct gttcgtcttc aaccgcctgt tgaagcgctt
     1861 ctaacgaggt gaaaaaagga atgacgctgg tgccgtcttc tttttcccag tgctgtaaat
     1921 caagcgcact atcttcaacc acagcctcgc cctgcgccgc cgtaccaggc acccagacgg
     1981 tggattccag tagagtacgg aaaaaggccg ggcggtgcgc cggttcggtt gctgcttttt
     2041 ccagcaggtc ttcaagttcg ttttttgttt cggacataag aaccacaatt cattcaacgt
     2101 tcggggcgca aatgcctgat gcgctacgct tatcaggcct acaagggatt cgcaatttgt
     2161 tgaatttgca gaatttgtag gccggataag gcgtttacgc cgcatccggc atattagttt
     2221 acgccgttaa cagattagct atcgtgcgca caccaagtcc cgtagcgccc gcagaccact
     2281 gttcaaccgg cgctttacgg taagtcgccg agcagtcgat atgcagccag ccttgctgat
     2341 agttctcaac aaagtgcgac aggaagcccg ccgccgtgct cgcgcctgcc ggatacgccg
     2401 cgcttccggt attgttcagt tcggcaaagt tagacggcag ctggctgcgg tggaattccg
     2461 ccagcggcag acgccagaat ggttcgttct cttgtgaggc actcgccagt aaacgaccgg
     2521 caagcgcatc gtcaaaactg aacagcgcgt gataatcatt acccagcgca gttttcgccg
     2581 ccccggtgag ggtcgccgca tcaatgatca tttccggttt ctgcgcactg gcatcaatca
     2641 gaccatcggc aagcaccaga cgcccttcgg catcagtgtt catcacttca acttttttac
     2701 cgttgcgata ggtgatgata tcgcccagct tgaacgcatt gccgctaatc aggttatccg
     2761 cacagcagag gaacagcttc acgcgcttgt tcagtccgcg cataatggca aatgccagcg
     2821 ccccggtaac cgttgccgcg ccgcccatgt ccgacttcat cgagtccata aacgcagtct
     2881 gtttgatgct gtagccgccg gagtcaaaag tgataccttt acctaccagg cacgcgtaca
     2941 ccggtgcttc tttatcgcca gttgggttgt aatccagcgc cagcaatacc ggagaacgtt
     3001 ctgaaccgcg cccgacagtg tgcagcccca tataaccttg ctcacgcaga tcttcgcctt
     3061 tggtgatccg ataagtcaca cgatcgcccg cgacgttgct gatcagatca acagcacgct
     3121 gtgccagttg cgatggtccc aattcttctg ccggtgcgtt gatggtgtca cgcacccagt
     3181 cgatgatcat caggcggtta tccagttcct ggcgctgggc atcgtccaga tccggccact
     3241 ccactttacg cgtgcctttc gggcctttat aaccttgcca gaatgcccag cagcgatccg
     3301 catcccagcc ttcaccgctt aactgaacat gcttgatgcc cagaccgtca atcttgcggg
     3361 ccgcacgctg gatcagcccc agatcgtctg ccccgttcaa atgcagggta atgccgtcat
     3421 tattaatgct gtaagttgct ttttctcccc agcgcgcgtc ggcaggttgg gtagagaggg
     3481 taatcttcat cgcttctgtc attttagtta tccttattcg tttaacagca aaacgggcta
     3541 cccccaggca gcccgttcat tcactcggct tcgtccagcc agactaacaa aatcgcttcg
     3601 aggattttct cgttggatgc ctgcgggtcg tcgtcgaaat cttccagatc gcaaatccac
     3661 tgatgcatat cggtgaatcg aaccgttttc ggatcaagat cgggatacgc atcgtacagt
     3721 gcttcgccaa tttcgcggct atcggtccac ttaagtccca tactaacctc tgttaatgct
     3781 cacgcgcatg gttgatagtg taacgcggga tttcgaccac taaatcttcg tcggtgacgc
     3841 gcgcctggca gcttaaacgg ctttccggct ccagtcccca ggctttgtcc agcatgtcgt
     3901 cttcctgctc tgagctttcc ggcagtgagt caaaaccttc acgaacgatg cagtggcagg
     3961 tggtgcaagc acaggatttt tcacaggcgt gttcaatctc aataccgtta cgcagcgctg
     4021 cgtcgagaat ggtttcaccg ctattagctt ccagaacagc gccatcaggg cagaggtcct
     4081 gatgaggcaa aataacaatc tttggcatat taaacctcgt ccacggaatg gcctttcagc
     4141 gcacgacgaa ccgactggtc catgcggcga gcggcgaaat cctgggtttg tttgtctacg
     4201 tttttaatcg cttgttcgat ggcgtcaaca tcatcgccct gcgccacttc actcaggtga
     4261 gcggcagcat tgtcaatgac ctgacgctct gcggcgctta acagcgcggc atcagcagcc
     4321 agcgcgccgt gcagactttc cagcacacgc gccgcttcta ctttttgttc tgccagcata
     4381 cgggctttta cgtcctgctc ggcatagctc attgagtctt tgatcatcga agcgatttcg
     4441 ctatcggtca gaccgtaaga cggtttgacc tgaatagacg cctcaacgcc ggtggatttc
     4501 tccatcgccg tcacgctcaa aagaccgtcg gcatcgacct ggaacgtcac gcgaatatgc
     4561 gcaccgccag ccggtagcgc cggaatacca cgcagcgcaa aacgcgccag tgagcggcag
     4621 tcctgcacca gttcgcgctc accctgcatt acatggatag acatcgccgt ctgaccatct
     4681 ttaaaggtgg tgaaatcctg agcgcgggcc accggaatag tggtattacg cggaatcact
     4741 ttctccacca ggccgcccat cgtttcgagg cccagcgaca gtgggatcac atcaagcagc
     4801 agcatttcgc tgtctggctt gttacccacc agaatatccg cctgaatcgc cgcgccaata
     4861 gcgacgactt tatccgggtc gatggaagtc agcggtggac gaccgaaaaa ttcgcctacc
     4921 cgttcacgca ccagcggcac gcgagtagaa ccgcccacca tcaccacttc cagcacttcg
     4981 tcagcttcta cacccgcgtc tttcagcgcg cgacgacaag ccagtaaggt tcgttttacc
     5041 agtggcgcga tcagttcatt gaattgttca cggctgattt cgccctgcca gcccgcaacg
     5101 ttaacggtca cggagtccgc atcgctcagc gcgattttgg ctgcaatggt ggcatccagc
     5161 agttcacgct gaacgcggtt atcgctacga tcaggaatgt ccgcctgctc gcgaatgtaa
     5221 tccgccagca gatggtcgaa atcatcgccg ccgagcgcgg aatcaccgcc ggttgccagc
     5281 acttcaaaca cgccgcgact taagcgcaga atggaaatat caaacgtccc gccaccgagg
     5341 tcataaacag cgatcacgcc ttcctgaccg gaatccagcc cgtaggcgat agccgcagca
     5401 gtcggttcgt taagtaagcg cagaacatgc aaccccgcca gacgcgccgc gtctttggtg
     5461 ccctgacgct gggcatcgtc aaagtacgcc ggaacggtga taactacacc atccagctcg
     5521 cctgccaggg cttcagttgc ccgcgccgcc agtgctttga ggatgtccgc agaaacgcgc
     5581 accgggttca gtagccccgc cgccgtttca atcatcggca ggccgttttc gctggcctgg
     5641 aattgataag gcagatgcgg atagcgttgc tggatatcag ccagcgagcg ccccatcagg
     5701 cgtttaacag aactaatggt gttggcggtg tcaagcgctg cgttagtacg cgcgtcataa
     5761 cccaccgaat gcccttgctg ttgatagtga acaacagatg gcagcaggtg acggccttca
     5821 tggtcggcta acgtttcggc ctgaccgctg cgcactgtcg ccaccagcga gttggttgtg
     5881 cccaggtcaa taccagccgc cagacgacgc tgatgcggcg cagcactcaa accaggttca
     5941 ctaatttgta ataaggccat gtttagcttc cagaaattaa aaatcgagca gtttttcttc
     6001 gagttgttcg gcactgctcc gcagtttatc gagaaaacgc agcttacgca ccgtatccgc
     6061 cgccgcgtcc cacgcctcgt tgtctaactg ttcaaccatc aactgatggc gggtatcaaa
     6121 catctttttc acacgtttga taaagctttc cagccgcgct tcatctttcg cctgttcgat
     6181 ctcgtccagc tcttcgcgca gctccaactg ttccatcagg aacgcggtgt cgcgcacagt
     6241 atgctgctcg ctggcgagat caaaaccgtg taaagaaagc aaatattccg cgcgcattaa
     6301 cggatgacgc agcgtttgcc aggcctggtt aatggttgca gattgctgta cggcggcgag
     6361 ttgttccgcc aggcttccgc tggcgaattt atcaggatga tactgacgtt gtagatcctg
     6421 aaaacgcagg ctcagtgcct gggtatcgag ttgataacgg gcaggcaagc caaagagggt
     6481 gaagtaatcc ataacattct cagggctgcg atccgccgcg ataacggcga atcaggtaaa
     6541 acaaacccca cgcgcaggcg accacggtgg ggttatcggt atgcgcatca aacgtggaag
     6601 ctttcgccgc aaccacactc atctttaacg ttcgggttgg tgaatttaaa cccttcattc
     6661 aggccttctt ttacgaagtc cagctgcgta ccgtccagaa attgcaggct tttgccatcg
     6721 accacgactt tcacgccttt gtcttcaaac acgatgtctt ccggcgtcgg ttcgtcaaca
     6781 aattccagta cataagccat acctgaacat ccggaggttc tcacgcccag acgcaggcca
     6841 aaccctttgc cgcggttagc cagaaaggta tttactcgcg ctgctgcact gtcgctcagt
     6901 gtaatcgaca taaccaaacc tcaactctta ttttgcttca cgtttgcttt tatagtccgc
     6961 aatggcggct ttgatcgcgt cttctgccag aatagaacag tgaattttca ccggcggcag
     7021 ttcaagttct tcagcaatat cggtgttttt gatcgcctgc gcttcgtcga gagacttccc
     7081 tttcacccat tcggtgacca gggagctgga agcgatagcg gaaccgcagc cgtaagtttt
     7141 aaaacgcgcg tcttcaatga taccttcatc gttgacttta atctgcaact tcattacgtc
     7201 gccacaggcc ggtgcaccca ccatgccgct gccgacgttc tcgtcgttgt tatcaaagga
     7261 acccacgtta cgcggattct cgtaatggtc gataactttt tcgctgtaag ccattataaa
     7321 ttctcctgat tccgataccg attaatgatg agcccattcg atgctgttca gatccacgcc
     7381 ctgcttgtac atttcccaca gcggagaaag gtcacgcaga cgaccgatgg atttacgaac
     7441 taactcgatg gtgtagtcga tctcttcttc agtagtaaaa cgacctaaag agaaacggat
     7501 agagctatgt gccagctcgt cgttcagccc cagcgcgcgc agcacgtagg acggttcgag
     7561 gcttgctgac gtacaggcgg aacctgaaga aactgcgagg tctttcagcg ccataatcag
     7621 cgactcacct tcaacgtagt tgaagctgac gttgagaatg ttcggcgcac cgtgttccag
     7681 gtcaccgttc aggtaaactt cttcgatatc tttgatgccg ttccacagac ggttacgcag
     7741 gccgcgcaga cgttccatct cggtcgccat ctcttctttt gcgatgcgat aggcttcgcc
     7801 cattccgacg atctggtgaa caggcagagt gccggaacgc ataccgcgct cgtgaccgcc
     7861 gccgtgcatt tgcgcttcga tgcgtacacg cggtttacga cgcacataca gcgcaccgat
     7921 acctttcggg ccatagattt tgtgaccgga gaaagacatc aggtcaactt tcaactggct
     7981 caggtcgatt ggcagtttgc ccacgctctg ggttgcatca acgtgataga taatgccacg
     8041 agcacggcac atttcgccga tagccgcgat atcctgcacc acgccgattt cgttatttac
     8101 gtgcatgatg gaaacaagga tggtgtcgtc acgcatcgcc gcttcgagtt ctttcaggtc
     8161 gataataccg ttacgctgcg gtgccaggta ggtgacttcg aaaccttcgc gctccagctg
     8221 acggcaagta tccagtaccg ctttgtgttc ggttttgctg gtgatgatgt gcttgccttt
     8281 tttctgataa aagttggctg cacctttgat cgccaggttg tcagattcgg ttgcaccaga
     8341 ggtaaagacg atttcacgcg gatcagcgcc gaccagatcg gcaatctgat tacgggcgat
     8401 atctaccgct tcttcagcct gccagccgaa acggtgagaa cgagaggccg ggttaccaaa
     8461 ggttccgtcc atcgtcatga actgcatcat tttctcggca acacgcgggt ccaccggcgt
     8521 ggttgcggag tagtcgagat aaatcggtaa tttcattgct ctataaactc cgtacatcac
     8581 tcaatgcaag gaatcaggct accggctgga tgtacgaccg tgtttacgga gtatttagca
     8641 ctccggcctg attctgaatt ctttttatta agcgcgtaac ttaacgtcga tcgcgtcttg
     8701 tgtgcgggtg cgtggcgcgt cgtgagtatg ctgacgacca gacacatcca gcacttcctg
     8761 gttattaacc agttcgccta aagtaatgtt gttgagaaaa ccggtgagac ggtcgctcaa
     8821 atcacgccac agcgcgtggg tcaggcattt atcgccgccc tggcagccgc ctttaccctg
     8881 acaacgggtg gcatctacag attcgtcaac ggcgctaatc acttcgccaa cggcgatgct
     8941 gctggcatct ttgcctaaca gataaccacc gcctggtcca cgtacgctgg aaaccagacc
     9001 atttttacgc agacgggaaa acagttgttc cagataagaa agggaaattc cctgacgttc
     9061 ggaaatatca gccaacggta ccgggcccgc ttcagagttg agcgcaacgt caagcattgc
     9121 ggtcacggca tagcgccctt tagatgtcag tctcatgtct tacttcacct caaactcgcc
     9181 cctgcccggg gttttttatt gtaaaagtgg gggtattgca tagcagggtc aagtctgaca
     9241 ttcccgagta aattggtcaa ctatttactt gactgattta gtcgggtatt taaccttcag
     9301 tgccattttt ttatcgtggc gtttgtacct gttgtcggat gcggtgtgaa caccttatcc
     9361 gacctacgat tctgtacatt gtaggcctga taagacgcgt taagcgtcgc atcaggcaac
     9421 cactgttatt cggccttgtt acctttattc tgctgctcaa tagaagccag aatcccgcgc
     9481 aggatattca attcctggct ttccgggcgc gcacgggtaa acagacggcg caatttattc
     9541 atcacctgcc ccggatggtt ttcacggata aaaccggttg ccagcagcgt ttgttccaga
     9601 tgaccataaa aacgctccag atcatcgacc agcggatacg gcgtctcttc atgctcgacc
     9661 tgctcgccat tttcctgagt tgccagccag gccatacgca cttcataggc aataacctga
     9721 accgccattg ccaggttcag cgaactgtat tccgggtttg ccgcaatcgc gacatgataa
     9781 tggcatttct gcaactcttc attggtcaag ccgacgcgct cacgaccaaa caccagcgcc
     9841 accggggtat ttgccgcttc agcgacgctt ttcagaccgc attcgcgcgg gtcgagcatc
     9901 ggccacggca gcgtgcggga gcgtgcgctg gtgcccacca ccaggctaca accggctaac
     9961 gcttcgtcga gcgtatcgac gatgtgagca ttaccaatca catcgctggc ccctgcagcc
    10021 agggcaatcg cctgggagtc gggtttcacc agtggattaa ccagccacag attggttaat
    10081 cccattgttt tcatggcacg ggcaacagaa cccatattgc cggtgtgtga cgtctccacc
    10141 agcacaattc gaatattttg cagcattgtc tttcttcatc taaagattat tcacgcatct
    10201 tatcataaaa cgaagacaga tgccgatctc gctgctatac tctgcgccgt tttcccgttc
    10261 tttaacatcc agtgagagag accgatgcat ccgatgctga acatcgccgt gcgcgcagcg
    10321 cgcaaggcgg gtaatttagt tgccaaaaac tatgaaaccc cggacgctgt agaagcgagc
    10381 cagaaaggca gtaacgattt cgtgaccaac gtagataaag ctgccgaagc ggtgattatc
    10441 gacacgattc gtaaatctta cccacagcac accatcatca ccgaagaaag cggtgaactt
    10501 gaaggtactg atcaggatgt tcaatgggtt atcgatccac tggatggtac taccaacttt
    10561 atcaaacgtc tgccgcactt cgcggtatct atcgccgttc gtatcaaagg ccgcaccgaa
    10621 gttgctgtgg tatacgatcc tatgcgtaac gaactgttca ccgccactcg cggtcaaggc
    10681 gcacagctga acggctaccg tctgcgcggt agcaccgctc gcgatctcga cggtaccatt
    10741 ctggcgaccg gcttcccgtt caaagcaaaa cagtacgcca ctacctacat caacatcgtc
    10801 ggcaagctgt tcaacgaatg tgcagacttc cgtcgtaccg gttctgcggc gctggatctg
    10861 gcttacgtcg ctgcgggtcg tgttgacggt ttctttgaaa tcggtctgcg tccgtgggat
    10921 ttcgcggcag gcgagctgct ggttcgtgaa gcgggcggca tcgtcagcga cttcaccggt
    10981 ggtcataact atatgctgac cggtaacatc gttgctggta acccgcgtgt tgtgaaagcc
    11041 atactggcga acatgcgtga cgagttaagc gacgctctga agcattaatg actcaggcgg
    11101 gtgatatcac tcacccgccc tcgcctttca ggcgctattc cgaaatactt cctcaccgct
    11161 ttactttctt tcgtcactct cccaccatct ttccccgatt aatggatgat aaagaagtcg
    11221 caaggaaagg ttatggcact gccagtgaac aaacgcgttc ccaaaattct gtttattctc
    11281 tttgttgttg ccttctgcgt ttatttagtg ccgcgcgttg ccatcaactt cttctattat
    11341 cctgacgata aaatttacgg tcccgacccc tggtcggcgg aatccgtcga atttacggct
    11401 aaggacggta ctcgtctgca aggctggttt attccttctt cgacgggccc tgctgacaac
    11461 gccatcgcaa ccatcattca tgctcacggc aatgccggaa atatgtccgc ccactggccg
    11521 ctggtcagtt ggttacctga gcgtaatttc aacgttttta tgtttgatta tcgcgggttt
    11581 ggtaaatcaa aaggcactcc gtcccaggcc ggattgctgg acgatacgca aagcgccatc
    11641 aatgtggtgc gccatcgcag tgatgtaaac ccacaacagc tggtgctgtt cgggcagagt
    11701 attggcgggg cgaatattct tgctgttatt ggtcagggtg atcgtgaagg catacgtgcg
    11761 gtgatcctcg actccacatt tgcctcttat gcaaccatcg ccaaccagat gatccccggc
    11821 agtggctact tacttgatga gagttacagc ggcgaaaatt acatcgccag cgtcagcccg
    11881 atcccgcttt tactcattca cggtaaagcc gatcacgtta tcccatggca gcacagcgaa
    11941 aagttgtata gcctggcaaa agagccaaaa cggttgatcc taatcccgga tggcgaacac
    12001 attgatgctt tttccgatcg tcacggcaat gtttatcgcg aacagatggt ggactttatc
    12061 cttagcgcgt tgaatccgca gaactaaccc atgatcgcta gcacgataat cattcacaaa
    12121 accaccttaa gacatgctaa tccactggtc agaacagttt aaaatgagaa aaattctgtg
    12181 acgcttgcca acatttctga tgattagcat tcccttcgcc atttccctga gcaaacttta
    12241 gctattctta tcaattatgc ttatgggaga tctacagatg atgcctacgc ttgctccacc
    12301 atctgtcctt tcggctcccc agcgccgctg ccagatcttg ctgacgctct ttcagccggg
    12361 gttgaccgcc accatggcaa ccttcagcga gcttaatggt gtggatgatg atattgccag
    12421 tcttgatatc agcgaaacag gacgggagat cctgcgctat catcaactca cactgacgac
    12481 tggttatgac ggtagctacc gggttgaagg tacagtgctt aaccaacgtt tgtgtttatt
    12541 tcactggcta cgacgtggtt tccgtctgtg tccgtcattt attaccagcc aattcacccc
    12601 cgccctgaag agtgaactga agcggcgcgg aattgcgcgt aacttttacg acgataccaa
    12661 tctacaagcg ttagtgaatc tctgctcccg acggctgcaa aaacgctttg aatcgcgcga
    12721 tattcatttc ctgtgtctgt atctgcaata ttgtttgctg cagcaccacg ctggaattac
    12781 gcctcagttt aatccgctcc aacgtcgctg ggcggaatcc tgccttgaat ttcaggtagc
    12841 gcaggaaatt ggacgccact ggcagcgtcg ggcgctccag catgtaccgc ctgatgagcc
    12901 actgtttatg gcattacttt tttccatgtt gcgggttccc gatccactgc gggatgcgca
    12961 tcagcgggac agacaattgc gtcagtctat caaacgtctg gtaaaccatt ttcgtgagct
    13021 gggaaatgtt cgtttttatg atgaacaggg gttatgtgat cagctttata tccacctcgc
    13081 ccaggcgtta aatcgcagtt tgtttgccat cggtattgat aataccctgc cggaagagtt
    13141 cgccagactg tatccacgcc tggtacgcac cacccgcgcg gcgctggccg gatttgaaag
    13201 tgaatacggc gtccatcttt ctgatgagga aagtggtctg gtcgcggtga ttttcggtgc
    13261 ctggctaatg caggaaaacg atctgcatga aaaacagatt attctactga ccgggaatga
    13321 tagcgagcga gaagcgcaga ttgagcagca gttacgcgaa ctaacgttac tgccgctcaa
    13381 cattaagcat atgtcggtaa aggcattttt gcagacaggc gctccgcgtg gcgcggcact
    13441 gattattgcg ccttatacca tgccgttacc gctcttttca ccaccgctga tctatacgga
    13501 cctgacgctg acaacacatc aacaggagca gatccgcaaa atgctcgaat cagcatgagg
    13561 gaacaacttt cgggcgcaaa aacattgccg gaagcgccac cagcgccatt acccagaaca
    13621 cgccgtggcc cagatattga tacaggaaac cggcgaaaac ggtcatgata gcgatactgc
    13681 cgcccatcgc gacggcagag taaaccgcct gtaaacggat gacttcgcta acctggcgag
    13741 cagcaatata acgcatggcg gccaggtggc agaccgtgaa ggtgccgcaa tgcagaattt
    13801 gcaccactat caaccacggc aacgccgtag ttgctcccat aatgccccag cgcactacgc
    13861 cgcaaatcgc cgagatcaac agcatatcgc gagcactaca acggcggaaa agtttattac
    13921 tcagcgcaaa gataatgact tccgccacca cgcccagcga ccacaaatac cccaccgccg
    13981 aggccgagta gccagctgcc tgccagtaaa tggcgctaaa accgtaatag gccgcatgtg
    14041 ccccctgcaa taaacaaacg caggccagaa agcgccagtt ctggcgaacc agcgccaacc
    14101 acgcagacca accggtgctc tcctgctggc ggcttgcccc ttgtggctga atcgtcggac
    14161 ggatgagaaa gccgagcagc atggatgcca cgcccaacgt caacagcacg agaatcaccc
    14221 gataatcaaa catagtgacc agtttgcccg tcagcgccga gccaatgaca aacgccaccg
    14281 agccccacag tcgcactttg ccgtaatcaa gcgggaactg cttttgccac gtattcgcca
    14341 gtgcatcggt caacggtacc agcggtgaga aaaagaggtt aaagccaatc atcaccagca
    14401 tcagccacgc tacgtgcgcc cccgcccaga aggcgacagc aaagagaagt ggcagcagtg
    14461 ccagcacgcg caaggcggaa atcaggcggg aaggatcgct gacgcggggc gcgatgagca
    14521 aactcccgag aaaacgggca accagacctg cccccaataa caggccgatg gtttctggcg
    14581 ttaaaccaat ccctttaagc cagacgctcc agaaaggtaa aaaaatgccg taactaaaaa
    14641 agtatgtgaa atagccgagc gccaaccagc gcgtggattg caaaaccatg agtccctccc
    14701 gtcaggaggc gttagtctgg ggttaatcga atgttggtgc aagttgaaaa ataagaatat
    14761 tcgagcctgc ttcccgatgt ctgtaacgcc agttcacaag cactaaaaaa ctgcgatccg
    14821 cgacgcatgt tgcaaaaata atgcgtaatt atgccgttac gcttgccaaa cgttcctgca
    14881 caatggcgat gaaatcgcgt aatgccagct tcatctcccc tttcttccac gccatcagta
    14941 aagcaatgga aggcacattt ccggcaatgg gacgaaaaac aacctgcccg gtattaaaat
    15001 tattcatata accgggtatc aaagtgacgc ccagccccat gcccaccaga ttcatggtca
    15061 ccagaatatt cgttgccacc tggacgatat ttggctggct gttttcttgc gcaaaccacg
    15121 ctttaacgat cggcgcaagc gaaccggaat acgccggatc ggtactgacg aaattcacgc
    15181 catccagttg cgcggcggtg atctcttttt catgtgctaa agggtgatca accggtaaca
    15241 ccaccactaa tggttcgtca aaaagctcca gataatcaat ctccgggcta taaacaggat
    15301 ggcgcattaa gccgacgtcg agttcaccac ggcgaatttt ttcctcctgt tgcgtggtga
    15361 ttaaactcac cagctcaatc aaggtgtctg gctgtctgag acgaaacatc ggtaatactt
    15421 ttggcaataa attcacttcc gccgatggca caaagccaat ggttaattgt ctgtcttcct
    15481 gaacaatttt ccgcgcccgt aatttggcat tttccgcttg ttcgaggatt gccagcgcgt
    15541 cctggagaaa acattctccc gccgccgtca acgcgacttt gcgcttatcc ctcaccagta
    15601 acggaacacc gacacagttt tcaagatcgc ggatctggct gcttaacgaa ggctgtgagg
    15661 tatgcagttt ttccgccgca cgggtaaagt tcagtgcctg cgccactgcg acgaaatagc
    15721 gtaaatgccg tagttccatc accttcccct tgttatcgaa aaaacgtctc agctggtaga
    15781 aattaactat ttcacatatt agcaaccaac cagcaacatc cttatggcac aaaaatagaa
    15841 ggtcaataca tcttatcttt caggattaaa aaatatgacc acaccctcag atttgaacat
    15901 ttaccaactg attgacaccc aaaacggtcg ggtcactccg cgtatttata ccgacccgga
    15961 catttaccaa ctggagcttg agcgtatttt cggtcgttgc tggctatttc tcgcccacga
    16021 aagccagatc ccaaaacccg gtgatttctt taacacctac atgggagaag atgcagttgt
    16081 cgtggtgcgc cagaaagacg gcagcatcaa ggcgtttctc aaccaatgcc gccaccgggc
    16141 catgcgtgtg agttatgcag attgtggcaa cactcgcgcc tttacctgcc cgtatcacgg
    16201 ctggtcttat ggcattaacg gcgagttgat cgatgtaccg ctggaacctc gcgcctaccc
    16261 acaagggttg tgtaaatccc actgggggct aaacgaagtt ccttgtgtgg agagttataa
    16321 aggactgatt tttggcaact gggataccag cgcaccgggc ctgcatgatt acctgggtga
    16381 cattgcctgg tatctggatg gcatgctgga tcgtcgcgaa ggcggcaccg aaattgtcgg
    16441 cggcgtacaa aagtgggtga tcaactgtaa ctggaaattc ccggcagagc agttcgccag
    16501 tgaccagtat catgctctgt tcagccatgc ttctgccgtt caggtattag gggcgaaaga
    16561 tgatggcagc gataagcgcc tcggtgatgg acaaaccgcc cgcccggtgt gggaaaccgc
    16621 caaagatgcg ctgcaatttg gtcaggacgg tcacggtagc ggtttcttct ttactgaaaa
    16681 accggatgct aatgtctggg tcgatggcgc agtttcaagc tattaccgcg aaacctatgc
    16741 cgaagcagaa caacgtttag gtgaagttcg cgccctgcgc ctggcgggtc ataacaatat
    16801 attccccacg ctttcatggc taaacggcac tgccacactc cgcgtctggc atccgcgcgg
    16861 ccctgatcaa gttgaagtgt gggcgttctg tattactgac aaagccgcct ccgatgaagt
    16921 taaagccgct tttgaaaaca gcgccactcg tgcttttggt cctgctggtt ttctcgagca
    16981 ggatgactcg gagaactggt gtgaaatcca gaaattgctt aaaggccacc gcgcccgcaa
    17041 cagcaaactg tgtctggaaa tggggcttgg tcaggaaaag cgccgcgacg acggcattcc
    17101 tggcattact aactatatct tttcagaaac tgccgctcgt ggaatgtacc aacgctgggc
    17161 cgatcttctg agtagcgaaa gctggcagga agtgctcgat aaaaccgccg cttaccagca
    17221 ggaggtgatg aaatgagtgc gcaagtttca ctggagttac atcaccgcat tagccagttt
    17281 ctctttcacg aagccagtct gctggatgac tggaaatttc gtgactggtt ggcacagctc
    17341 gacgaagaga ttcgttacac catgcgcacc acggttaacg cgcaaacacg cgaccgtcgc
    17401 aaaggcgtcc agccgccgac gacctggatt ttcaatgaca ccaaagacca gctggagcgg
    17461 cgaatcgccc gtctggaaac gggcattgcc tgggcagaag agccgccgtc acgcactcgt
    17521 cacttaatca gcaactgcca ggtaagcgaa accgacatcc caaacgtatt tgccgtgagg
    17581 gtgaattatc tactttatcg ggcacaaaaa gagcgcgatg aaacattcta tgttggaacg
    17641 cgtttcgaca aagttcgccg tctggaagat gacaactggc gcttgctgga acgggatatc
    17701 gtcctggatc aagcggtgat cacttcccat aacctgagtg tactgttctg atgaatcgaa
    17761 tttatgcgtg tcccgttgcg gatgtgccgg agggtgaggc tctccggata gatacctcgc
    17821 ccgtcatcgc cttgttcaac gtgggcggcg agttttatgc cattaacgat cgttgcagcc
    17881 atggtaatgc gtcgatgtca gaagggtatc tggaagatga cgccacggtg gagtgcccgc
    17941 tacacgccgc cagtttttgc ctgaaaacgg ggaaagcgtt atgcctgccc gccaccgatc
    18001 cgctcaccac ttatccagta cacgttgaag gtggtgacat tttcatcgac ttaccggagg
    18061 cgcagccatg agcgatctgc ataacgagtc catttttatt accggcggcg gatcgggatt
    18121 agggctggcg ctggtcgagc gatttatcga agaaggcgcg caagttgcca cgctggaact
    18181 gtcggcggca aaagtcgcca gtctgcgtca gcgatttggc gaacatattc tggcggtgga
    18241 aggtaacgtg acctgttatg ccgattatca acgcgcggtc gatcagatcc tgactcgttc
    18301 cggcaagctg gattgtttta tcggcaatgc aggcatctgg gatcacaatg cctcactggt
    18361 taatactccc gcagagacgc tcgaaaccgg cttccacgag ctgtttaacg tcaacgtact
    18421 cggttacctg ctgggcgcaa aagcctgcgc tccggcgtta atcgccagtg aaggcagcat
    18481 gattttcaca ctgtcaaatg ccgcctggta tcctggcggc ggtggcccgc tgtacaccac
    18541 cagtaaacat gccgcaaccg gacttattcg ccaactggct tatgaactgg caccgaaagt
    18601 gcgggtgaat ggcgtcggcc cgtgtggtat ggccagcgac ctgcgcggcc cacaggcgct
    18661 cgggcaaagt gaaacctcga taatgcagtc tctgacgccg gagaaaattg ccgccatttt
    18721 accgctgcaa tttttcccgc aaccggcgga ttttacgggg ccgtatgtga tgttggcatc
    18781 gcggcgcaat aatcgcgcat taagcggtgt gatgatcaac gctgatgcgg gtttagcgat
    18841 tcgcggcatt cgccacgtag cggctgggct ggatctttaa ggaagcacga tgaaagaaaa
    18901 aacgatcatt attgtcggtg gcgggcaagc ggcggcaatg gctgcggcct cgctacgcca
    18961 gcaagggttc accggtgagc tgcatctgtt ttccgatgag cgacatcttc cttatgaacg
    19021 acctccactc tcgaaatcca tgttgctgga agattccccg cagttacagc aggtgttacc
    19081 cgctaactgg tggcaggaaa acaatgttca tctgcattcc ggtgtaacca tcaaaacgct
    19141 gggtcgcgac acacgagagt tagtgttaac caacggcgaa agctggcact gggatcagct
    19201 ttttatagca accggcgcgg cagctcgacc gctgccgttg cttgatgcac tgggagaacg
    19261 ctgctttacc ctacaccatg ccggtgatgc cgccagactg cgagaagttc tgcagcccga
    19321 acggtcagtc gtgattgtcg gtgccggaac tattggtctg gaactggctg ccagcgccac
    19381 gcagcgcaga tgtaaggtga cagtgattga actggcggca accgtcatgg gccgtaatgc
    19441 accaccgccc gtgcaacgct atcttttaca gcgccatcag caggctggtg tgcgcattct
    19501 gctcaataat gccattgaac atgtggtcga tggtgaaaaa gtagaactga cgctgcaaag
    19561 tggggaaacg cttcaggctg atgtggtgat ttacggtatt ggtatcagcg ccaacgagca
    19621 actggctcgc gaggccaacc ttgatactgc caatggcatt gtcattgatg aggcttgccg
    19681 cacctgcgat cccgcgatct ttgccggtgg cgatgtggca atcactcgtc ttgataatgg
    19741 tgcactacac cgctgcgaaa gctgggaaaa cgccaataac caggcgcaaa ttgccgctgc
    19801 cgcaatgtta gggctaccgc taccgctact gccgccgccg tggttctgga gcgatcagta
    19861 cagtgataac ttacagttta ttggcgatat gcgtggcgat gactggcttt gtcgtggcaa
    19921 cccggaaact cagaaggcga tttggtttaa tctgcaaaac ggcgtgctta tcggtgcggt
    19981 cacgctgaat cagggccgtg agattcgccc aattcgcaaa tggatccaga gcggcaaaac
    20041 gtttgatgcg aaactgctga tagatgagaa catcgcgctt aaatcactgt aatcaggata
    20101 attagcgaat atctcaatgc ctggggcgtg gcgaggtgca agagtgtgta ttacgtttaa
    20161 atcacattat cttgcaaagg gattggttat gaacacacta cgttattttg attttggagc
    20221 tgcccgcccc gttttgttat taattgcccg tatcgccgtg gtcttaattt tcattatttt
    20281 tggttttccc aaaatgatgg gctttgacgg tacggtccaa tatatggcct cgttgggcgc
    20341 gccaatgccg atgctggcag cgattattgc ggtagttatg gaagtgcccg ccgcgatatt
    20401 aatcgtgctt ggctttttca cccgtccgct ggcggtgctg tttattttct acacgctggg
    20461 tacggcggtg attggtcacc attactggga tatgaccggc gatgcggttg ggccaaatat
    20521 gattaatttc tggaagaatg tcagtatcgc tggcgcgttc ttgctattgg caattaccgg
    20581 accgggggca atttctctcg atcggcgtta ggaaagatgc cggatgcggc gtgaacgcct
    20641 tatccggcat tttataaatt acagccactc tacccgcaac gacatctctg aggttgttga
    20701 ttcccctggt gctagcgcaa tgagatcacc cccttccggg cgatgatgat catccggcgc
    20761 atggctcatc ggttccagac agaagaaatc aaacgcatat cctttatcaa acgcagggtc
    20821 ggaaacaaag atgaaataac acggtgcagg tggcgtcgtt tccatgatga tggcataacc
    20881 ttcctgcggc tgctcgatgc gggcctgacc attccatccg gcaaaaccat tgttcaccca
    20941 ctgacgcggc aacggcgcga gctggttaaa atccagttcc tgcggtagct gctcgcaaaa
    21001 ctcacccgcc agccactgct cccgttccag ccagtaaccg ctcgcctgcg cctgaatccg
    21061 cgtttgtggt gacaacggaa aataaggatg ccagccggta ccaaatggca gcgtctctgc
    21121 cccttgattg gtgacagaga gcgtcaccgt cagcgtatcc gccgttaaat gaaacgcctg
    21181 acttacccga tagtgataga caccgctgcg atgttcatac accagacaca aactatcatc
    21241 gctgtgcgag acacattgcc actcgcccag ccagccatcg ccgtgtagat agtgcgcatc
    21301 ccactcaaca ttcggttgca gttgatactc acgcccctgc cagacaaaac ggttgccgct
    21361 cacccggttt gcaaatggca ccagcggaaa acatgaggca tcggttgcca caccgctttt
    21421 tttaccagga cgtaagagcg gcgtcgtatc gcgccagaag ccttcgatta cgccgccctg
    21481 gtcagaaacg tccagcttta gcgacccgtg ggataaggta tagatggtca tccgctaact
    21541 ccttaatccg ggaagttaat cacaactttc ccgcatttgc cgctcgccat cagcacatag
    21601 gcatcacctg cctgttctag cgagaagcga tgggtaatgg cgttacgcgg ccacagcttc
    21661 cagtcagtca aatcgtgggc gcatttttcc atatggaaca gactggtcac ccaggagccg
    21721 ataatccgcc gttgatggtg catcagatca gcgctgacct cgaattccac ttttccggtt
    21781 tcaccaatgt aaaccacccg cccccagtca gcagtggatt gcagtgccag caagcgacct
    21841 gcggcattac cggaacaatc gagcgcaaca tccgcgccac cgtgggtaag ttcagcaatt
    21901 atctgcggca gaccttcggt ggttgctaaa tagccgtgat ccatcccccc taactgtttt
    21961 gccatcgcca gacgttccgg cagcatatca acgccgatga tccgttttgc accgcgacct
    22021 ttcgccagca tcatcgccat catgccgacc ggccccagac cgaccaccag cacgttatcg
    22081 ctgccggaaa cttcgccgcg caaaatccct tcataggccg tgccaacgcc gcaactgata
    22141 aacgcaccat cttcgtagct cagcgcatcc ggcaggagga tcagatcttt ttcttccgcc
    22201 agcagatatt cggcatgacc gccgtcacgc tgccagccgt aagccgcttt tcctttgcca
    22261 gtacaagaga taggaaagcc gcggcggcag ttcgggcaaa aaccacagcc agaaatgtga
    22321 tacaccagca cgcggtcgcc ctctttaaaa tggcggcagc cttgccccat cgccacaatc
    22381 tgcccgcacg gttcatgacc gttgataaat ccctggtata acggtttatc gggtgccgcc
    22441 gccgtggcac ggtgttgatg atagatatag tggacatcgc ttccgcaaat cccggaggat
    22501 ttcattttga tcagtacctg gttaatcccc ggcgtcggca ccgcaacttc ccgcagatcg
    22561 acggttgaat ttcctagtaa ataagcagcc agcatcgttt tcataaatcc ctcattaaca
    22621 atacgattaa ttttcatccc tgccgcaccc gcgccagggg cgttaaggtt tagcgtttcg
    22681 ctttactgct tcgctgggtc agcaagatat tcgccagcac cgccaccacg atgatgacgc
    22741 cgcgtaccac ctgctggaaa aaggagttaa taccgagcag caccagaccg ttaccgatta
    22801 gcgtaatcac cagcacacca agcaatgtac cgaacaggga accgcgaccg ccggaaagtg
    22861 ccgtaccgcc gaccacgacc gcggcgatga cgtcaaactc cagaccgttt gcggcacctg
    22921 cgttaccgga accgaggcgt gccgccaaca aaatgccggt caccgccgct aataatcccg
    22981 aaagggtaaa gataagaatg cgcactcgac gaacgttaat accgcacaac tgcgccgccg
    23041 tggcattacc gccaacggca aaaaccgagc gcccgaaggc ggtttttcgg ctaatgaaca
    23101 caaacagcgc aaacaacacc atcatgatca gcgcggatac tggcacaccg agaaattgtc
    23161 cgcccagcca gtccagcacc tcgttttcgt caatcggcac tggcagtgcg ttggtcataa
    23221 acagccccat tccgcgcaga gcgctccaca gccccagtgt ggcaacgaaa cttggtacgt
    23281 taaacacgcc gcgcagcacc ccggcgagcg ttcccatcag cgcgcctaac agcaacacca
    23341 gcagacaagc aatcgccaac ggaacttcaa attgcagcaa aaatgccagg cacaccgaga
    23401 caaaagccac catcggccca acgctgacat caatttcacc ggagataata atcagcgtca
    23461 tcgcccaggc ggcaatccca atggtggcgg catcgcgcag cacgttcatc tggttattca
    23521 atgagataaa gccaggcgcg ttcagggaga agaccagata aagaatggca atcaccacca
    23581 gcaaaccgat ctcattaata tggcgactga caaattgttt gagcgagacg ctcttgccct
    23641 gcggcaatgg taatgacgaa gcatacatct tcaggttcct cggtaaaatt catcagtgta
    23701 cagacagaat ggcggacatc agctcatcca cattgaccgg cgagtgaaac tcctgcgaga
    23761 acgtgccgtg ctgtaataac aggatgcggt cacacaccag cggcaattcc tccacttcac
    23821 tggagataaa caccacgctt tttccttcgg cagccagctc acggacaata cggtaaatct
    23881 gctgtttggc ttcgatatcg acgccgcgcg ttggctcgtc gagcaacaaa atctggctgg
    23941 cggcatagac ccaacgaccg atcaccactt tttgctgatt gccaccagaa agcgtgccga
    24001 tgggtgtttc gctactggca gccttgaccg tcatccgctg catcacctct tcggtcaggc
    24061 ggcggatggt ggaccattgc agcacaccgt tggtgctgat tttttgccga ttggtcagca
    24121 ctgtattttc gtcaacgcct agccagggaa tgatccccgc ttctttgcgg ttttctggcg
    24181 tatagccaat gccgcgtttc agcatgtcgc cgtaatcggg gcgcatgatt ttctcgccgt
    24241 tgataacaat ttcgccctgt tcatacgtct ccagcccaac aatcgccttc agcaattcac
    24301 tgcgccctgc ccccagcagg ccagcaatgc caagcacttc gccacgacgt agcgtaaagc
    24361 tgatatcctc cagcttgggc ttatggcgta acgcacggac ttccagtacg gcctgatcca
    24421 caatttcctg aggggcaacc ggcgcaatat caacgtgatc acgccctagc attagcgaca
    24481 caatatgatg cgtggaggtg ttttcgagca tcacatcgcc cgccacctga ccatcgcgca
    24541 taacggtggc ataggaggca atgcggcgga tttcttccat ccggtggctg acataaatca
    24601 ccgccacgcc cagtgccgac atctttttca ccgcactgat caccagttca acttctgcac
    24661 tggcaagcga gctggtgggt tcatcaagaa tgaccacgcg cggctcgccc ttcatcaccc
    24721 gtgcaatttc caccagttgc ttttgcgccg ggcttagcgt tgaaacaagt tgttcaggac
    24781 taacgtcaac gcccagcgcc tgtaagcaac gttgggcatc ctgcgccatt tgcaggtaat
    24841 caatcatgcc gttgcggcga ggccattgac caaggcagag gttttctgcc accgtcagcc
    24901 cttccaccag acttaattcc tgataaaccg cacgaacccc cagttcagcg gcacggcgag
    24961 tcaacgtagc ttcgtcacct tccagtcgcg tctcgccaat ccagatatca ccgctatccg
    25021 ggcgttcgct gccggtaagc attcgaatga gagtcgattt gcccgcgccg tttttaccta
    25081 acagcgcacg aacttcgcct ttattgagcg tgaagttaac gttatccagc gcaacgacgc
    25141 cggggtaacg cttatttcct gccaccactt ttgctaccgg gactgcctct gttgccgtga
    25201 acatagggac ctctgcgaat cagcgattag ggcagaccat caacgtgcgt tgccagccac
    25261 tgtttaccat cttccgtttt ggtatagaga tcgattggca cctgaatcac tttttcaccg
    25321 tcggcttgtt tattgataac cttcaatgtt tgcgcgaaaa cagcattgcc cattttctta
    25381 ccggaaatat ccactaccgc tttcagcacc tgattgtttt ccagctcctg agcaatttcg
    25441 gttgtcatat ccgaaccgaa aacagcaatt tttccggcct gattttgatt acgtaccgct
    25501 tttaccgcgc cgagtgtcgc accgcccgac tcccccataa tggcgttgag atccggcgtg
    25561 gagataatca gtttttcacc aacggaaatc gctttatcta aaacagtccc ttcctggtta
    25621 gcgacaattt gcgcgccggg aacgcgggat tttaatactt cttcaaatcc tttacgtcgc
    25681 tgcacacaaa cttcaaaggc ttcgcaattg atgacggcaa ttttcggctg gtcaatttta
    25741 ttggcaataa aataatcggc ggcagcgtta cccagttttt taccaaattc cagtggatcg
    25801 ccgaccagat acgccgagac atatttatcg acaccctttt gattaataca ggtgttgtag
    25861 caaatcaccg gaatacccgc ttcactggcg cgacgaacgg tacggctact gccattttca
    25921 gacactgccg ataaaataat ggcatcgaca ttacgcgcca cgagggtatc aacaaaggta
    25981 ctttctttcg aaatatcccc ctgggcgtta gtttcaatta actgtacctg tactgaagaa
    26041 tcttttgccg catcctgaac gccctgacgc actccagcgt aatatccctg ggtatcaagg
    26101 tatattgcac caatggtcat ttctttatcc gcagccctgg caaatagtgc gctacctaat
    26161 agcgtagcca ttaataatag attacgggtt gttctcattt ttttaggcat agtgcctcct
    26221 gtagggtttt tattaacaac ggcttattct aattattttg tgatgagcgg cagcgcgtgc
    26281 cgctcaaata ttacagttaa tagggtttag tgaacgtagt taaaaataaa aggcatcaca
    26341 gtggtgaata atgccgcttt cggccaggcc ggattaatcg ccgtcaattc agcgcgagca
    26401 gactcatatt gcgcaacctc cccgagtccc gcggacgcca gcatcgccac catcaggcat
    26461 ttttctttat gctgctgttg taaatcgcca tacagcgata acaggtcagg ttgtgaaaca
    26521 gcaaagaaat ccgcctcaat actggttttc gccatctctt gcgcccactg tttcatttca
    26581 ctaaacagtt gctgtgcggt ttgctgttca cccagcagtc gcagcgccat tccttgccag
    26641 aagagataat caaccggctg atcgttgtaa taactgtgga tgttaatggt gcgatcgccg
    26701 gtcgccgcca gacgtaaaca acgcatcgct tcagtttcat cgccctgcgc gttggcgcat
    26761 atcgcctgcc agaaccagat gtcgttatca gtttgccccg gtaaacggcc ttcgcttaaa
    26821 ttctccggat aatacagcgc ggcatgaagc agttcgctgg cctgctgcgg ctctctggca
    26881 tcaagatgct gccaggcgcg taataactgg ttgaggataa actgactggt gaccttccct
    26941 tccccgcctt cccacgggta gaatttacgc gtggcgagaa tgtccgccgc tttgtctgcc
    27001 tgacccgtga gatgccacaa attgagcagt tctgcggtca tgtcgtcgcg tttcagcgca
    27061 atttccagat tattttccag acgcgccagt cgtttctccg gtgtggctcc acttaactta
    27121 tccagcaaat cccgttcgaa aagcagacgt gcatcctgcg gcgcaagctg ataagcatta
    27181 tcaagataac gcgcggccag ctcataatcg tgtttcttat tccacgcatg gatcgctaac
    27241 ccgcgccagc cgtcggcaaa ctccggcgac atctctacgc aacgttgcca taaggcaatg
    27301 gctttgttgt agctacgttt gttgtagtag aagcaggcca gtaaatggcg agcaaaccag
    27361 cactcttcaa tactctccag cgccgccact tcttccagcg tattcgggaa acggacaaac
    27421 tgcgggaaga catcaatggc ttttgcgacc agttcgccac gttcggcttt cggcagcaag
    27481 ctggcttgca ggtaaagcgg cagcgtgcgc tggcagtcca gtgcgttcag catctctgcc
    27541 gccagggtgg gcattcccca gttaatcagt tgcccggcgg tcatcagagc gttaacgtcg
    27601 cgtccctgac acagaccgcg ccactgcgcg agggctgatt cgctacgacc atcgaaccag
    27661 ttcagccacc acagagtggc gttcagcgga taatcacgca gcagtttctc gcgctgcaaa
    27721 cgcgcgttgt cctgacgacc acttaacacc agcagcagat tatgcaagca aagcacttcc
    27781 tgattggttg ggcaggcacg aagactttgt tggcaaaaat ccagacccgc gtcgaagtta
    27841 tcattacgcg ccgccagtcg tgccagacca taatagccgc ctgctttact attgccgctc
    27901 cagaccgcac gccagaaatc ctcttcggct tgttgatatt gtccctgacg ttcgtaagca
    27961 ctggcgcgaa tcaaacttgc ttgtccacac tgcgggtttt tgttcagcgc atgtgcgcgt
    28021 ttcagcgcct gactggcata cgccaccgct tgcgggaaat ctgcgcggtt atattccagc
    28081 atcgccagcg ccaggttaca gcgataatcc agcgggtcca gcgccacgcc gcgcaggtag
    28141 taatcgaacg gtgaacggct ggcgtgatga tattgctcca gatgctgacc gataaaccag
    28201 gcttcatctg tactggtaat gtcttgtgct gccagtggcg ctttggcgac gtccggcaac
    28261 ggcaactcct gcggctgatg ttcctgataa ctcagtacaa tattgccgtc ggcatcggag
    28321 agttcaatgg tcagtctgtc aggattgata ccgtgcaaca cgccctggat ggcggtcgct
    28381 ggtgtcaggg ccacggcatc atcgagcaac gcgttgcatt tgccgatttc gcggatcgcc
    28441 agacggtatc cgttcaacgg agagatggca tacagccccc actcaatccc ccgctcacta
    28501 cgctggagtt ttatcaccgc atcgcgggag gcattttgca ccatgcccag agaatgataa
    28561 ggcaggaaat actgctcgaa acgcttctct tcgtaagcat caagccaggt aaaatcaggc
    28621 tggttatcgg caaaaatacc ggtcatcagt tcgatatacg ggccgttatt gtcggtcaga
    28681 ctcttatccc acgcctggcc aaattcactg tgtccccaac tccactgttt tttacctggc
    28741 gcaatatggt ggttggcaac gtgcagcaaa ccgccatctt catcgtgaca ccacgcgccg
    28801 acaaaatcgt actgcgattt ttcggccata tatgaggttg gaacaggcac atttttatag
    28861 cgagaaatgt ccactccggc ggagtagtcc actttgtagt aagtgccggt ggcgatgggg
    28921 aaagcggaga cggcccgttt gccgtgatca aacaccgcag tcacatccgg tgggaaaacg
    28981 ctctgatgcc cttcaccccc tttcactgcc gggttggccc accacaagaa atgacgcggc
    29041 gtggcgttgc cgttatagac gcggctggcg atttccagtg ccgcccggtc agggcgcagg
    29101 gtgaaacctg tcatcacctg taaaccatgc atcggctccg tttcgccgac ccacaccgtc
    29161 tgtgcaccgt cttcatgggc ttcgagggtg aaatcaacgg gcataaaggt ggtcgggcga
    29221 tggtgttgcg gccagttaaa ctcaatcccg ccggagatcc acggtcccag catccccacc
    29281 agcgcaggtt taatgacttc attgtgatag acaaaatcgc gctgtttcac tttatcccat
    29341 gcgcgatgca cccgaccgcc cagttccggc aggatcatca ctttgatgta gtcgttttcc
    29401 agccacaccg cctgctagga tttcagggtt ttctgctcgc tcagcgtatc ggtcacgccg
    29461 tagggataaa ccgcgccgga cgatccctga taaacgcgat tttccaggaa catgggatgt
    29521 atatcctgcg gcccggtttc ataggtcggg atctcaacgc gctcttgcca cacttttact
    29581 ggagtcatgg tgccctcaat attaacaaga catactgaat taaaagattt gtggcagtgt
    29641 attgaacaat ctggcaatgt tttcgcggaa taatcacgca attaactaaa caaggtttag
    29701 tgaagatgag agcctgcatt aacaatcaac agattctcca ccataacaaa tgcgtgattc
    29761 tggaactgct gtaccggcaa aagcgcgcca ataaatcaac gctggcccgg ctggcgcaaa
    29821 tttcgattcc ggcagtcagt aatattttgc aggaactgga aagcgaaaaa cgggtggtga
    29881 atattgacga tgaaagccag acgcgcgggc atagtagcgg tacatggctg attgcgccgg
    29941 aaggtgactg gacgctgtgc ctgaacgtga cgcccaccag tattgagtgt caggttgcta
    30001 atgcttgttt aagtccgaaa ggcgaatttg agtatttaca gattgatgta ccgacgccgc
    30061 aggcgctgct gtccgaaatc gaaaaatgct ggcatcgcca ccgtaaattg tggccggacc
    30121 gcaccatcaa tctggcgctg gcaatccacg ggcaggttga tccggtgacc ggcgtatcgc
    30181 aaaccatgcc gcaagcgccg tggacaacgc cagttgaggt gaagtatctg ctggaagaga
    30241 agctgggcat tcgggtgctg gtcgataatg actgcgtgat gctggcgctg gcggagaaat
    30301 ggcaaaataa ttcgcaggaa cgggatttct gcgtgatcaa cgttgattac ggcattggct
    30361 cgtcgttcgt gattaacgag caaatttatc gcggcagttt gtatggtagc ggacagattg
    30421 gtcacaccat cgttaatccg gatggcgtcg tctgcgactg tggacgttac ggcagcctgg
    30481 aaaccgtcgc ctcgttaagt gcattaaaaa aacaggcgcg ggtatggcta aaatcacaac
    30541 cggttaatac tcagcttgag cctgaaaaac tgacaaccgc gcagttaatc gctgcctggc
    30601 agagtggaga accgtggatc accagctggg ttgatcgctc tgccaatgcc attggtttga
    30661 gtctgtataa cttcctcaac atcctcaata ttaatcagat ttggttgtac ggtcgcagtt
    30721 gtgcctttgg tgagaactgg cttaatacta ttattcgcca gacaggattt aacccgttcg
    30781 accgcgacga aggaccgagc gtgaaagcga cgcaaattgg cttcgggcaa ttaagccgcg
    30841 cacaacaggt gctgggaatt ggctatttgt atgttgaggc gcagttacga cagatttgat
    30901 ggcgcgataa cgtagaaagg cttcccgaag gaagccttga tgatcataaa cgaaaaattg
    30961 cctgatgcgc tacgcttatc aggcctacac ggagattgca atatattgaa tttgcaagat
    31021 tttgtaggcc tgagaaggtg ttcatgccgc atccaacatg aacaacgagc acattgtcag
    31081 caaatcaccg tttcgcttat gcgtaaaccg ggtaacgtgc gcagatgtcg agaactttac
    31141 ctttgatgcg ctcgataacg gcttcatcat tgatgctgtc cagcacgtca cacatccagc
    31201 cagccagttc tttcgcttcg gcttctttaa agccgcgacg ggtaatcgcc ggagtaccta
    31261 cacgaatacc ggaggtcaca aacgggctct tcggatcgtt cggtacgctg tttttgttga
    31321 cggtgatgtt agcacggccc agagcggcgt ctgcttcttt accggtcagg tttttatcaa
    31381 ccagatcaac caggaacagg tggttatcag tgccgccgga aaccactttg tagccgcgct
    31441 cgaggaacac ttctaccatc gctttagcgt ttttcgcgac ctgctgctgg taagttttga
    31501 actcaggctc catcgcttct ttcagagcaa ccgctttacc ggcgattacg tgcatcaacg
    31561 gaccgccctg accaccaggg aaaacggcag agttcagttt tttgtacagc tcttcgctac
    31621 cacctttcgc caagatcagg ccgccgcgcg gacccgccag ggttttgtga gtggtggtag
    31681 taacaacgtg agcatgagga accgggttcg ggtagacgcc agcagcaacc aggcccgcaa
    31741 cgtgcgccat atcaacgaac agataagcac cgatgctgtc agcgatttca cgcattttcg
    31801 cccagtcaac cacgccggaa tatgcagaga aaccaccgat aatcattttc ggcttgtgtt
    31861 ctttggcttg tttttccaga tcggcgtaat cgatatgacc ggtagcatcg ataccgtaag
    31921 gaacgatgtt gtacagttta ccggagaagt taaccggaga accgtgagtc aggtgaccgc
    31981 catgcgccag gttcataccc agaacggtat cacctggttc cagcagcgcg gtgtagaccg
    32041 caaagttagc ctgggagccg gagtgcggct ggacgttagc gtagtcagcg ccgaacagtt
    32101 ctttcgcacg atcgatcgcc agttgttcaa cgatatcaac atactcgcaa ccgccgtagt
    32161 agcgtttgcc cggataacct tcagcatatt tgttggtcag ctgagaaccc tgcgcctgca
    32221 ttacgcgcgg gctggtgtag ttttcggagg cgatcagttc gatgtgctct tcctgacgta
    32281 ctttttcctg ctccatagcc tgccacagtt cggcatcata atcggcaatg ttcatttcac
    32341 gctttaacat ccgcatctcc tgactcagct aacaataaaa tttttggcct ttataggcgg
    32401 tcctgttgga caacggcgaa cagtataacc gaatcattgt gcgataacag gtcttgacaa
    32461 aggaatttac gcaaacgatt accttcaggc tacgcaaggc tttggagaat aaagagcttg
    32521 caaccggaaa cggatttctt ttcaggtttg tgatgcaaat ttttcacttc atcacattct
    32581 ttctgaaaaa caccaaagaa ccatttacat tgcagggcta ttttttataa gatgcatttg
    32641 agatacatca attaagatgc aaaaaaggaa gaccatatgc ttgacgctca aaccatcgct
    32701 acagtaaaag ccaccatccc tttactggtg gaaacggggc caaagttaac cgcccatttc
    32761 tacgaccgta tgtttactca taacccagaa ctcaaagaaa tttttaacat gagtaaccag
    32821 cgtaatggcg atcaacgtga agccctgttt aacgctattg ccgcctacgc cagtaatatt
    32881 gaaaacctgc ctgcgctgct gccagcggta gaaaaaatcg cgcagaagca caccagcttc
    32941 cagatcaaac cggaacagta caacatcgtc ggtgaacacc tgttggcaac gctggacgaa
    33001 atgttcagcc cggggcagga agtgctggac gcgtggggta aagcctatgg tgtactggct
    33061 aatgtattta tcaatcgcga ggcggaaatc tataacgaaa acgccagcaa agccggtggt
    33121 tgggaaggta ctcgcgattt ccgcattgtg gctaaaacac cgcgcagcgc gcttatcacc
    33181 agcttcgaac tggagccggt cgacggtggc gcagtggcag aataccgtcc ggggcaatat
    33241 ctcggcgtct ggctgaagcc ggaaggtttc ccgcatcagg aaattcgtca gtactctttg
    33301 actcgcaaac cggatggcaa aggctatcgt atcgcggtga aacgcgaaga gggtgggcag
    33361 gtatccaact ggttgcacaa tcacgccaat gttggcgatg tcgtgaaact ggtcgctccg
    33421 gcaggtgatt tctttatggc tgtcgcagat gacacaccag tgacgttaat ctctaccggt
    33481 gttggtcaaa cgccaatgct ggcaatgctc gacacgctgg caaaagcagg ccacacagca
    33541 caagtgaact ggttccatgc ggcagaaaat ggcgaagttc acgcctttgc tgatgaagtt
    33601 aaggaactgg ggcagtcact gccgcgcttt accgcgcaca cctggtatcg tcagccgagc
    33661 gaagccgatc gcgctaaagg tcagtttgat agcgaaggtc tgatggattt gagcaaactg
    33721 gaaggtgcgt tcagcgatcc gacaatgcag ttctatctct gcggcccggt tggcttcatg
    33781 cagtttaccg cgaaacagtt agtggatctg ggcgtgaagc aggaaaacat tcattacgaa
    33841 tgctttggcc cgcataaggt gctgtaattt gatgttgccg gatggaaaca tccggcaacc
    33901 cttgacgcgg tttaaattgc cgcgtcgtcc tcttcaccgg tacggatgcg aatgacccga
    33961 gccacgtcaa agacgaagat tttaccgtca ccgattttgc cggtttgcgc cgtgcgaata
    34021 atggtatcga cacaggtatc gacaatgtcg tccggtacga caatctcaat tttcactttc
    34081 ggcagaaaat ccaccatata ctccgcgccg cggtacagct cggtatggcc tttctggcga
    34141 ccaaagcctt tcacttcggt caccgtcatg ccggtaatac cgacttcggc cagtgcttcg
    34201 cggacatcgt ccagcttgaa gggttttata atcgcatcaa tctttttcat gctattcctt
    34261 gaaaaggttg cctgtctttt gatctgctaa acgtaacaca taacgccaat tcattccttg
    34321 aaatcgtttg catccagctc gtgtcgggaa agcagtttat aaaattctgt ccggttgcgc
    34381 cccgccattc tagccgcgtg ggtgacgttg cctttggtga tttgcagcag cttacgcaaa
    34441 tagttgagtt caaaatgatt acgtgcctca acaaaggttg gcagcgccgt attttcaccc
    34501 tccagcgcct gctccaccag cgcatcacta atcaccggag atgaggtcag cgccacgcac
    34561 tgttcaatca cgttgaccaa ctggcgcaca ttacccggcc agctcgcggt catcaggcgt
    34621 ttcatcgcat cggtagagaa cgcgcggaca aacggtttat gtcgctctgc cgcctggcgc
    34681 aacaggtgat ttgccagtag cggaatgtct tctgtgcgct ccgccagtgc cggaattttc
    34741 aggctgacaa cgttgaggcg gtaatagaga tcttcgcgga attccccgcg cgccatcgct
    34801 tttgacagat cacggtgagt ggcagaaata atccgcacat tgatatcaat atcgcggtta
    34861 ctgcccagcg ggcgcacttt acgctcctgc aacacgcgca gcagtttgac ctgtaacggc
    34921 gcaggcatat cgccaatttc atcgagaaat agcgttccgc cttccgccgc ctggaataaa
    34981 ccttcgcgat tgctgacagc gccagtaaac gcgccacgcg catgaccaaa cagctccgac
    35041 tccagcaatt gctcgggcaa tgcgccacag ttaatagcaa taaatggttt gctgttgcgc
    35101 gggctggcgt tgtggatagc ctgggcgaaa atctctttcc cggtgccgct ctgaccgtta
    35161 atcaaaacgc tgacgtctga ttgcgccacc agccgcgcct gttccagcaa acgcagcatc
    35221 agcgggctgc gggtgacaat tgcctcgcgc cagcgttcat cggtggctgg cgcggattgc
    35281 tccagcgcat cgtcaattgc ctgatatagc gcatctttat cgacaggctt ggtgaggaaa
    35341 ctaaaaacgc cctgctgtgt tgcagcaacg gcatcgggga tagaaccatg cgcagtaaga
    35401 ataattactg gcattcccgg ctgcactttc tggatttcag caaatagctg cataccgtcc
    35461 atttcatcca tccgcaggtc gctgataact aaatctactt tttcgcgatt cagtacccgt
    35521 aatccttcag cgccactttc cgccgtgacc acactgtagc cttcgctggt caggcgcagg
    35581 ccaagcagtt tcagcaatcc cggatcgtca tcgaccaata ataaatgcgc aggtttatgg
    35641 ctcatcagga gtgacctcat gggtggatgg cgcgggcttt tcactctcgt gcggcgtatc
    35701 cggcgagaaa tttccagccg gtttgcgggt cgagagctgg cgttcaatat cggtcaggtt
    35761 ttccagcttg cgggtggtga gttccagttg ctgctgtaga acgtgatgtt gctggcgcaa
    35821 tgtatccagc tcgctgtcgc tggactgctg gagtttactg taacgttggc gctcttccgc
    35881 cagttgcagt tgcagcgcct gaccgtcgcg ccagagttga tacagtgggc gaacctgtgc
    35941 cgggatctcg gtacttaacg cttcaatacg cgcgaccagc tggcggcgct catacggcgt
    36001 aattttggcg tcggcgagca aaatcccttg tttaaaggta ttttgccagc tgccgtcgtc
    36061 atattggcga gcttgctgac gcgactgcgc aggcattaaa cgatcagcac aatccatcgc
    36121 ccgcagccag taaagcggat tggtttcggt tgatttacct tgcagcgccc agatgtcgct
    36181 acattcagta gaaagatagt cagccagttg ataaaccgga attttttctt ctgctggcgt
    36241 atcaatggct ggcttattgt gattctgcac gcaacccagc aatgccagac atggcagccc
    36301 tgccagccac agccgtcggg gcaataatcg ttgaaaaatg tgtcgcatat tcaccagact
    36361 taaagcctat cccagtgggc gtaattgttg cagacagtct ggacatggac agcgcggaga
    36421 aaccggggcg tacatatcgt acgtgaagat ttcgagcact acccggggcc gaactgacaa
    36481 ataaaatagc ctgataggat aggctgttag cattatttcg tgtttttcga cgacggtaat
    36541 tcaatgcgga aacaaacgtc ttgcccgctc tcgtcgacca gatacagttc cccttgcata
    36601 cggcgaatac aatccctggc aatgcttaat cccagaccgc tgcccttcac cgcccctttt
    36661 cgctggtggc ttccctgaaa aaaggggtcg aagatcatgg cgcgttcctc ttgcggaatg
    36721 ggcgtgcctg tattgatgac atcaatataa acccgcgcac catgtaaact gctgcgaagg
    36781 caaatgttac cggattcagc cccgtagtgc accgcattgg agtaaagatt atccagtacg
    36841 ctcatcagca gcattggctc cgccaggcaa gctgttgctt tgagatcgac gtcggtatgc
    36901 atcattttag cccgtgcggg caggctatga gcagaaacca ctgtctccac cagcggtgct
    36961 aattcaacat tctccagttc caccgcactg tccgcctgtt tacggttgta atcaagcagt
    37021 tgttcgatca gtttttgcaa attgcagctg ctgctatcaa gaatgctcac cacctctttt
    37081 tgctctggcg taagcggccc gacaacctga tcagccagta attcagtgcc ctcgcgcata
    37141 ctcgccagtg gcgtttttaa ttcatgagat aaatgtctta aaaattgatg gcgttgggat
    37201 tccagccatg acaggcgctc acttaaccaa agaatacgtt gcccaaccga gcgtaactcg
    37261 ctcggtccac agaacgaaac gctattgccc agagaacgcc cttcccccag acggttgatc
    37321 atgcgctcga tatttttcac cggcccgata atcatccgcg tgaaaagtag caccatcacc
    37381 agactcacca gaaatagcac cagcgattgc caaccaaaat attgcccacg ttcggcgatt
    37441 tcacgctgaa gttgctgccc acgagagaac accactgtgc gcgtggcctg caccatttcg
    37501 gtattggcac tggcaaaggc ttcaagacgc gcggcggcgg cagcatcggg accactgttg
    37561 ttacactgaa gttgggccag attgttcaaa tcctgacgca atgcctggta aagtttatcg
    37621 tctggcagta cgcctgcgtg ggcatcgagc atttcgctgt aacgcttgcg ctggctttga
    37681 taaaccttcg caagcgtagg gtcgtccagc acgcaatact gacggtagct acgctccatc
    37741 tccagcgccg cgttggtcat cgcttcacta cgccgggcat caataagcgt agtgcggtta
    37801 accagcgccg cctgatcgct aagcgcattc aggctttgcc aggcttgcca tgccagcacc
    37861 aacaggggta gtagaattag caaaaatgcc agcattacca gttgtcgtaa tgagcgggga
    37921 aaaacgggcc agcgtttcaa ggtgttactc tcgtcagacg cgaatagcct gatgctaacc
    37981 gaggggaagt tcagatacaa caaagccggg aattacccgg ctttgttatg gaataaggcg
    38041 gtgcctaact cgacgtttcg cccgatggtt gatatagcta cgctgatatc agaagttgga
    38101 cggcaggcac cttgttgtgc gtcattcgaa ttttatgtag cacgtcccga aggggctgac
    38161 ataagtcggt gaatgagcca ctgcttacta ttatgcagta actgtgccaa taaagaaaat
    38221 agtttggtaa cgtactgatt atacgttgct ttgagggatt tatggcttct tcgctatttg
    38281 aatgtaaatc agccactgtg tcgctaaaaa gagacaactt aagataaact tattagatta
    38341 caataaaatc aatcatttaa gtgtcgccag aaagcgacac ggtaaaccac ccattgtcgt
    38401 gatttacaga cacaaaaaag ctcccggagt tgggagctta tgatagtggt tggtgcttaa
    38461 tgccggatgc ggcgtaaatg ccgcatccgg gctgcaaaac caatgaactg atgacttacc
    38521 ccaactgctt acgcgcattg cggaaaatgc gcatccatgg gccatcctcg ccccagtttt
    38581 ccggatgcca ggagttgctg acagtacgga aaacacgttc cgggtgcggc atcataatgg
    38641 tgactcgacc actttcagtc gtaaccgccg taataccgtt cggtgaaccg ttcgggttag
    38701 ccgggtaggt ttcagtgact ttgccgaagt tatcgacata gcgcagtgcc accagccctt
    38761 tgctttccag tgccgccaga tgcgccgcat cacgcacttc cacgcgccct tcaccatgag
    38821 agacagcaat cggcatttgc gagcccacca tcccctgcaa cagcagagac gggctttggg
    38881 ttacttcaac caggctgaaa cgcgcttcaa agcgatcgga ggtgttacgc acaaaacgtg
    38941 gccacaactc actccccggg atcagttcac gcaggttaga catcatctgg caaccgttac
    39001 atacccccag ggacagcgtt tgcggacgat ggaagaaggt tgcaaactca tcgcgtacac
    39061 ggtcattgaa caggatagac ttcgcccaac cttcaccggc acccagcaca tcaccgtagg
    39121 agaaacctcc gcacgcgacc agggcgtgaa aatcctccag tcccgtacgt ccagtcagca
    39181 ggtcgctcat atgcacgtcg atagcatcaa agcccccacg ttggaaagct gccgccattt
    39241 caacatgcga gttcacgccc tgctcacgca gtacggcaac tttcggacgt gcgccagtgg
    39301 caatatacgg tgctgccaca tcttcgttga tatcgaacga cagttttaca ttcaggcccg
    39361 gatcggcgtc gttagatttc gcctgatgct cctgatcggc acactccggg ttgtcacgca
    39421 ggcgctgcat ctgccaggta gtttctgccc accagacacg caacgtagtg cggctttcgc
    39481 tgaatacagt ctgcccgttg gcggtaatca caaaacggtc accggaaacc gcctgcccaa
    39541 cataatggac gcaatccgca agcccatgct gtgccagtac ggactcgacc gcttcacggt
    39601 cagcggcacg aacctgaatc accgcaccca gttcttcgtt aaacaacgcc gccaggcgat
    39661 cgtcacccag agaggcgata tccgcgtcaa tgccacaatg acctgcaaag gccatttccg
    39721 ccagcgttac cagcaggcct ccatcagagc ggtcgtgata cgccagcagc ttacgctgtg
    39781 caaccagcgc ctgaatcgcg tcatagaagc ctttcagttg gacaacatcg cgtacatctg
    39841 ccggtttgtc gccaagctga cgataaacct gcgccagcgc cgttgcgccc agcgcgttat
    39901 tgcctttgcc caaatcaatc agcagcagtg cgttatcttc ggtacaaagc tgcggcgtga
    39961 tggtgtgacg tacatcttcc acgcgggcaa atgcagaaat caccagcgac agcggcgacg
    40021 tcatttcgcg ctcttcgtta ccttcctgcc agcgggtttt catcgacata gagtctttac
    40081 ccaccgggat cgtcaggccc agcgccggac aaagttcttc acccacggct ttaacggctt
    40141 catacaggcc cgcatcttca ccagggtggc ctgccgccgc catccagttg gcggaaagtt
    40201 taatgcgttt gatatcgcca atctgtgttg cggcaatatt ggttaacgct tcaccgaccg
    40261 ccagacgggc agaggcggcg aaatccagca gcgcaaccgg cgcacgctcg ccaatcgcca
    40321 tcgcttcacc gtagtagctg tcgaggctgg cggtagtgac cgcgcagtta gcgaccggca
    40381 cctgccacgg ccccaccatc tgatcgcgcg ccaccatgcc ggttacgctg cggtcaccga
    40441 tggtcaccag gaaagttttc tccgccacgg tcggcagatg cagtacgcgt ttcaccgcgt
    40501 ctgcaatggt gatcccttca cggaccagcg catcgccctt cgctttcagc gtttgtacat
    40561 cgcgggtcat cttcggcgtt ttaccaagca ggacgtccag cggcagatcg atcggctgat
    40621 tatcaaaatg acgatcgtgc agagaaagat gcagttcttc ggtcgcttca ccaatcaccg
    40681 cgtagggtgc gcgctcacgc ttacacagtt cgtcaaacag cggtaattga tcggcagcaa
    40741 ccgccagcac gtagcgttcc tgggattcgt tacaccagat ttccagcggg ctcatgcccg
    40801 gttcgtcgct tagaatatcg cgcagttcaa atttaccgcc gcgtccgccg tcgctcacca
    40861 gctccggcat cgcgttagaa agaccgcccg caccgacgtc gtggataaac agaattgggt
    40921 tagcatcacc aagctgccag caacggtcga tcacttcctg gcaacgacgc tccatctccg
    40981 ggttgtcgcg ctgtacggaa gcaaagtcga ggtcggcatc agactgacca gacgccatgg
    41041 aagacgccgc gccgccgcca agaccgatgt tcattgccgg gccgccgaga acgaccagct
    41101 tcgcaccgac gttgatctcg cctttttgta cgtgatcggc gcgaatgttg ccgatcccgc
    41161 ccgccagcat gatcggtttg tgataaccgc gcagctcttc gccattgtgg ctgttcactt
    41221 tttcttcata agtacggaag tagccgttca gtgccggacg accaaattcg ttgttaaacg
    41281 ccgcaccgcc cagcgggcct tcggtcatga tgtccagcgc agtaacaatg cgctcaggct
    41341 taccgaaatc ttcttcccac ggctgttcga agccaggaat tcgcaggttg gatacggaga
    41401 aaccaaccaa accagctttc ggcttcgcgc cgcgcccggt ggcaccttca tcgcggattt
    41461 caccgccgga accggtcgcc gcgcccggcc acggagaaat cgccgtcggg tggttgtgag
    41521 tttcgacttt catcaggatg tgcgccggtt cctgatggaa attgtagcgg cccgtttcgt
    41581 ggtcagcaaa gtagcggccc acttcagaac cttccattac ggcggcgtta tctttataag
    41641 cagagagaac gtgatctggc gtggtttcga aagtattttt gatcatcctg aacagcgatt
    41701 tcggctgttg ttcaccgtcg ataatccagt cggcgttaaa aattttgtgg cggcagtgct
    41761 cagagttcgc ctgagcgaac atatacagct cgatgtcgtt cgggttacga ccaagctttg
    41821 tgaaagcatc ctgcagatag tcaatttcat cttccgccag agccaagcca agacgcaggt
    41881 tagcgtcgat cagcgcctga cggccctgcc ccagcaaatc aacgcttgtg accggagtcg
    41941 gttgatggtg ggcaaacaac tgctctgcat catctaaatc aaaaaagacc gtttccatca
    42001 tgcggtcgtg cagttcagcg gtaacctgct gccattgttc attggtcagc gtaccggctt
    42061 ctatatagta agcaacgccg cgctcaaggc ggtttacctg ttgtagcccg cagttatggg
    42121 caatatcggt cgctttcgaa gaccagggag agatggtgcc aggacgcggg gtcaccagca
    42181 ggagtttgcc ttgcggggcg tggctggcga gtgccggacc atatttcagc aggcgttcaa
    42241 gttgtgcgtg ctcatcatcg tttaacgacg cattgaggtc agcaaaatgg acatactcgg
    42301 cgtaaatatt gtgaaccggg agcctggcag cctgaaaacg tgccagcagt ttgttgattc
    42361 ggaatgccga cagtgcaggc gaaccacgca gaatttccat cataagtctc tcgtcttcta
    42421 agctttcggt gtacccaagg ggggggaaac gggcgtcatt ataaagaatc tgatgcgctg
    42481 acgaaaccgt ttgcgtggaa ataaaatcac catcgtgaat tagcaacgcg tgccgccaat
    42541 ggctgtaata agttgccatc tggcgcaggt ttacgcaaaa tgccgctcat ttatgagtaa
    42601 acctttcact attattacgt tttttcaagc tgggacgcgc acgacacaga gaattaacta
    42661 attgaaaaaa ttaaagatta attatctgtt catcggcatt ctggcactgc tgctcgcggt
    42721 cgctctctgg ccatccattc cctggtttgg taaagccgac aaccgtatcg ccgccattca
    42781 agcgcgggga gagttgcgtg tgagcaccat tcatactccc ctgacttata acgaaatcaa
    42841 cgggaaacct tttggcctag attacgaact ggcgaaacag tttgccgatt acctcggcgt
    42901 aaaactgaaa gtgaccgtgc ggcagaatat cagccagctg tttgacgacc ttgataatgg
    42961 taacgccgac ctgctggcgg caggactggt ctataacagt gagcgggtaa aaaattatca
    43021 gcctggccct acctattatt ccgtgtcaca acaactggtt tataaagtgg gtcagtatcg
    43081 cccacgtacg ctgggcaacc tgacggcgga gcaactcacc gttgcaccgg gtcatgtggt
    43141 ggttaacgat ctccagaccc tgaaagaaac aaaattcccg gaattaagct ggaaggtaga
    43201 cgacaaaaaa ggctctgcgg aattaatgga agatgtcatc gaaggaaaac tcgattacac
    43261 cattgctgat tctgtcgcca tcagcctgtt tcagcgcgtt cacccggagc tcgccgtagc
    43321 gctcgatatc accgatgaac aaccggtgac ttggtttagc ccgttagatg gcgataatac
    43381 cctttccgcc gccctgctcg acttcttcaa cgaaatgaat gaagacggta cgctggcacg
    43441 cattgaagag aaatacctgg ggcatggcga tgattttgat tacgtcgata cgcgcacatt
    43501 tttacgcgcc gtcgatgcgg tactgccgca gttaaagccc ctgtttgaga aatacgccga
    43561 agaaattgac tggcgtttgc tggccgctat tgcttatcag gaatcgcact gggatgcaca
    43621 ggccacttca ccgacgggtg tgcgcggcat gatgatgtta accaaaaata ccgcgcaaag
    43681 cctcggcatt acggatcgta ccgatgccga acagagcatc agcggtggcg tgcgttattt
    43741 gcaggatatg atgagtaaag tgccggaaag tgtgccggag aacgaacgga tctggtttgc
    43801 cctcgctgcg tacaatatgg gctatgcgca tatgctggat gcccgcgccc tgacggcaaa
    43861 aaccaaaggg aatcctgaca gttgggctga cgtaaaacag cgtctgcctt tacttagcca
    43921 gaaaccctat tacagcaagc tgacttacgg ctacgctcgt gggcatgaag cctacgctta
    43981 tgtcgaaaat attcgtaagt atcagattag cctggtgggt tatctgcaag agaaagagaa
    44041 gcaggctaca gaagcggcga tgcaactggc gcaggattat ccggcggtat cgcctacgga
    44101 gttgggcaaa gagaaatttc cttttctctc gtttctttcc cagtcgtcat caaactattt
    44161 gacccattct ccctctctgc tgttttccag gaaagggagt gaagagaaac aaaattaatc
    44221 cgtcgaggat tgcgcttttt tctgcgcttt aatttcctgg cggcgcatgc gaaagaagtc
    44281 actgagcaac gccgcgcact catccgccag tattccttcc gtaatttcca ctcggtgatt
    44341 catacccgga tgatgcagca catccattaa agatcccaca gcgccagttt tcgcgtcacg
    44401 cgcaccaaag accacgcgac caatgcgact gtggatcatc gctccggcac acattacaca
    44461 tggttcaagc gtgacataca acgtggcgtc gatcagacga taattttgca tcaccagacc
    44521 accctgccgc agggccatga tttctgcatg tgcggtggga tcatggcgac caatcgggcg
    44581 gttccagcct tcgccgatta cccgattgtt atgcactaat accgcgccga ccggcacttc
    44641 ccgctcatcc caggcacgtt tcgccagcgt cagcgcgtga cgcatccaat attcgtggct
    44701 aaattcgact tcagacaaaa agaaaactcc ggttataaaa gcgcggcgca ttatacacgg
    44761 acgctatgct ttactctatt ccagttgctg gagttcaccg cgcggggtta ctcgccaacg
    44821 atgctgacag aaataaagca gcggattgtc ctgattactg tcgctatagc cactgtacag
    44881 ccgcagcggc gtgccgattt tgcgctccag ttgtgcgacc ttttcatgtc ccagacaacg
    44941 catcgtcaat acccaaccac catagccacg ctgaatttgg ctggcgataa gattaacccg
    45001 cggcagccag ggcgtatcga aataaaccgc ttcaaccagc ggctgcggag agccggtaat
    45061 caaccagata tcagcatcgg aacttaacag gtaggtggtt aatcgctcct gaaccagcgg
    45121 aaaggcggta acattgtcgc gaaaccagcg cacgaaatcg gcctgcaacg tctgtaaacg
    45181 tgcttcgctg tgaccaaaag tgcaccccca cagaagcaga ctcatcggcc agcgtgccgc
    45241 acgacctttt atcaataacg caatggctat aatcggtaac aacggcagga caagtaacgc
    45301 attcagcggt tggcgacgca gtaaatagcg cagaaaactg ccgaacatat cctgctgatg
    45361 caatgttcca tctaagtcaa aaaacaccac acgacgctcg tgagttgcca aaccatactc
    45421 ctctggactg aaacatcctg attaattttc gctactacat agcctaacag atagatcatc
    45481 acttttccgg caacaatcga ccgcatcatg gctaactgga atttttaatt catggcaatt
    45541 agcggcaatg gaatataaaa ttcactcgcg tgtgtctcat attgcctgtt gtcgccacta
    45601 tttcatcgcg aggaaagata tgaacggctt acttcgtatc cgtcagcgtt accaagggct
    45661 tgcccagagt gataaaaaac tggcggatta tctgctgcta caacctgata cggcgcgcca
    45721 tttaagctct cagcaactgg ccaacgaagc cggagtcagt cagtccagcg tcgtgaagtt
    45781 cgcgcaaaaa ctcggctata aaggttttcc ggcgcttaag ttggcgttga gtgaagcgct
    45841 ggcaagccag ccggaatcgc cctccgtgcc cattcataac caaatccgcg gtgatgatcc
    45901 gttacggctg gtcggcgaaa aactgattaa agaaaatact gccgctatgt acgcaacgct
    45961 aaacgttaat agtgaagaga aactgcatga atgcgtaaca atgttgcgct ctgcgcggcg
    46021 gataattctg accggtattg gcgcttcggg tctggtggcg caaaactttg cctggaagct
    46081 gatgaagatt ggcttcaatg ctgccgcact gcgcgatatg catgcgctac tcgcaacagt
    46141 acaggcgtcg tcccctgacg atctgttatt agccatttcc tacaccggtg tacgacgcga
    46201 gttaaacctg gcggcagatg agatgctgcg agtgggcgga aaagtgctgg cgattaccgg
    46261 ctttactccg aatgccctgc aacagcgtgc ttctcattgc ctgtatacca ttgccgaaga
    46321 acaagcgaca aacagtgctt caatctctgc ttgtcacgct cagggaatgt taacggattt
    46381 gctgttcatt gcgctgattc agcaggatct ggaactggca ccagaacgta ttcgtcatag
    46441 tgaagcgctg gtgaaaaaac tggtctgagt aaagaatgcg cgtataatgc ccgcccggtt
    46501 tgtgttgttt tgagagtttc cttatggcgc tgttaatcac taaaaaatgc atcaattgtg
    46561 atatgtgtga aatcaaataa aaacacattt attttcatag tgatacagca atgtgttatt
    46621 gaatgtgttg ttggatttat ggtgcaaaaa aattttgcat aagttcagca acacaaagaa
    46681 caccactttc tttaacgtcc tccgcgccct aaaggacggg gctttacggc gcaaaaggcc
    46741 tgtgacatac aacattaact tttcagtcaa ccgtagcacg ttcttgtata cgccgtgtct
    46801 gcggttttat ataaccctgg ctggaaacct cttatacaaa gtcgattcgc caacatcata
    46861 gatgatcgcc accttctggc gagaaactcc tgatgcaatt agtcgcccgg cctgcgccca
    46921 ttattctggt gaaagtttgg gacggcgacc gccaattcgt ccctgtgcgc gagcagcttc
    46981 cagtcctgct tttgttcgtt caacaatcag ttcacgttcc atttcagcca gggcacccat
    47041 cacatggaag aaaaagcgtc ccattggtgt gctggtatca attgaatcag tcagactacg
    47101 aaagttgatg cctcgttcgc gcaactcctc caccagcacg acaagatgcc gcatactgcg
    47161 ccccagccga tccagcttcc agacaaccag tgtgtcgcct gccgataatg tcttgagcag
    47221 ttttttcagc cccggtcttt cggactttgt accacttatc ttgtcttcaa aaatcagctc
    47281 gcatcctgca cagttcaacg cattacgttg tagatcggta ttctggtcat ttgttgatac
    47341 gcgtacatag ccaataagca tggtaaatcc ccctgttaac aagacactgc gtcaaaagtc
    47401 ggggg
//
LOCUS       DAJTQY010000023        46610 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.023, whole genome shotgun sequence.
ACCESSION   DAJTQY010000023 DAJTQY010000000
VERSION     DAJTQY010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 46610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46610
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.023"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(15..602)
                     /gene="mog"
                     /locus_tag="OO349_001324"
     CDS             complement(15..602)
                     /gene="mog"
                     /locus_tag="OO349_001324"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HCR5866465.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(717..1670)
                     /gene="tal"
                     /locus_tag="OO349_001325"
     CDS             complement(717..1670)
                     /gene="tal"
                     /locus_tag="OO349_001325"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCR5866466.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            1949..3378
                     /locus_tag="OO349_001326"
                     /pseudo
     CDS             1949..3378
                     /locus_tag="OO349_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
     gene            3448..4224
                     /gene="yaaA"
                     /locus_tag="OO349_001327"
     CDS             3448..4224
                     /gene="yaaA"
                     /locus_tag="OO349_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HCR5866467.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPANARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEDSSSNGELVFKRYEQR"
     gene            complement(4377..4673)
                     /locus_tag="OO349_001328"
     CDS             complement(4377..4673)
                     /locus_tag="OO349_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCR5866468.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPSPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(4887..6173)
                     /gene="thrC"
                     /locus_tag="OO349_001329"
     CDS             complement(4887..6173)
                     /gene="thrC"
                     /locus_tag="OO349_001329"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HCR5866469.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIADDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSFGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFSALRKLMMNHQ"
     gene            complement(6174..7106)
                     /gene="thrB"
                     /locus_tag="OO349_001330"
     CDS             complement(6174..7106)
                     /gene="thrB"
                     /locus_tag="OO349_001330"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HCR5866470.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVAPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVVSGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(7108..9570)
                     /gene="thrA"
                     /locus_tag="OO349_001331"
     CDS             complement(7108..9570)
                     /gene="thrA"
                     /locus_tag="OO349_001331"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HCR5866471.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTSVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(9596..9715)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(9651..9716)
                     /gene="thrL"
                     /locus_tag="OO349_001332"
     CDS             complement(9651..9716)
                     /gene="thrL"
                     /locus_tag="OO349_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HCR5866472.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(9930..10616)
                     /locus_tag="OO349_001333"
     CDS             complement(9930..10616)
                     /locus_tag="OO349_001333"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCR5866473.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTALAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(11016..11156)
                     /locus_tag="OO349_001334"
     CDS             complement(11016..11156)
                     /locus_tag="OO349_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5866474.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            11252..11968
                     /gene="arcA"
                     /locus_tag="OO349_001335"
     CDS             11252..11968
                     /gene="arcA"
                     /locus_tag="OO349_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HCR5866475.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(12027..13379)
                     /gene="creD"
                     /locus_tag="OO349_001336"
     CDS             complement(12027..13379)
                     /gene="creD"
                     /locus_tag="OO349_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HCR5866476.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFLLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLQLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREASESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFILALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(13437..14861)
                     /gene="creC"
                     /locus_tag="OO349_001337"
     CDS             complement(13437..14861)
                     /gene="creC"
                     /locus_tag="OO349_001337"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HCR5866477.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAHG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMGGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLDQALGNLLDNAIDFTPESGCITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(14861..15550)
                     /gene="creB"
                     /locus_tag="OO349_001338"
     CDS             complement(14861..15550)
                     /gene="creB"
                     /locus_tag="OO349_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HCR5866478.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDIHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(15563..16036)
                     /gene="creA"
                     /locus_tag="OO349_001339"
     CDS             complement(15563..16036)
                     /gene="creA"
                     /locus_tag="OO349_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HCR5866479.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            16247..17116
                     /gene="robA"
                     /locus_tag="OO349_001340"
     CDS             16247..17116
                     /gene="robA"
                     /locus_tag="OO349_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HCR5866480.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(17113..17760)
                     /gene="gpmB"
                     /locus_tag="OO349_001341"
     CDS             complement(17113..17760)
                     /gene="gpmB"
                     /locus_tag="OO349_001341"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HCR5866481.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWVERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            17812..18332
                     /gene="yjjX"
                     /locus_tag="OO349_001342"
                     /pseudo
     CDS             17812..18332
                     /gene="yjjX"
                     /locus_tag="OO349_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
     gene            complement(18417..18743)
                     /gene="trpR"
                     /locus_tag="OO349_001343"
     CDS             complement(18417..18743)
                     /gene="trpR"
                     /locus_tag="OO349_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HCR5866482.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(18833..20770)
                     /gene="sltY"
                     /locus_tag="OO349_001344"
     CDS             complement(18833..20770)
                     /gene="sltY"
                     /locus_tag="OO349_001344"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HCR5866483.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLIQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSTLTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            20981..22648
                     /gene="ettA"
                     /locus_tag="OO349_001345"
     CDS             20981..22648
                     /gene="ettA"
                     /locus_tag="OO349_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HCR5866484.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(22755..22922)
                     /locus_tag="OO349_001346"
     CDS             complement(22755..22922)
                     /locus_tag="OO349_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5866485.1"
                     /translation="MSFFDELKTSLEEAVEIKQGLKKPARVTRHEIEDAKAVVDRKRC
                     SRRIRHSVLNA"
     gene            complement(22956..24188)
                     /gene="nadR"
                     /locus_tag="OO349_001347"
     CDS             complement(22956..24188)
                     /gene="nadR"
                     /locus_tag="OO349_001347"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463436.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HCR5866486.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQG"
     gene            complement(24209..25591)
                     /gene="radA"
                     /locus_tag="OO349_001348"
     CDS             complement(24209..25591)
                     /gene="radA"
                     /locus_tag="OO349_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HCR5866487.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSIMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSGDLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(25640..26608)
                     /gene="serB"
                     /locus_tag="OO349_001349"
     CDS             complement(25640..26608)
                     /gene="serB"
                     /locus_tag="OO349_001349"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HCR5866488.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            26714..27358
                     /locus_tag="OO349_001350"
     CDS             26714..27358
                     /locus_tag="OO349_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HCR5866489.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            27386..28401
                     /gene="lplA"
                     /locus_tag="OO349_001351"
                     /pseudo
     CDS             27386..28401
                     /gene="lplA"
                     /locus_tag="OO349_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
     gene            28433..28696
                     /locus_tag="OO349_001352"
     CDS             28433..28696
                     /locus_tag="OO349_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5866490.1"
                     /translation="MIDLENQEREIINLMFSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPSWMTAEHK"
     gene            complement(28857..29576)
                     /gene="deoD"
                     /locus_tag="OO349_001353"
     CDS             complement(28857..29576)
                     /gene="deoD"
                     /locus_tag="OO349_001353"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCR5866491.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(29633..30856)
                     /gene="deoB"
                     /locus_tag="OO349_001354"
     CDS             complement(29633..30856)
                     /gene="deoB"
                     /locus_tag="OO349_001354"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCR5866492.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(30908..32230)
                     /gene="deoA"
                     /locus_tag="OO349_001355"
     CDS             complement(30908..32230)
                     /gene="deoA"
                     /locus_tag="OO349_001355"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HCR5866493.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVILAKDENSWQEAAKAVKAAIKLADNAPESTPTVYRRISE"
     gene            complement(32357..33136)
                     /gene="deoC"
                     /locus_tag="OO349_001356"
     CDS             complement(32357..33136)
                     /gene="deoC"
                     /locus_tag="OO349_001356"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCR5866494.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGDEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            33394..34935
                     /locus_tag="OO349_001357"
     CDS             33394..34935
                     /locus_tag="OO349_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HCR5866495.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQNEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIANGPV
                     HDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYCQQQ
                     IAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEGIAA
                     RYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGARLPY
                     GDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMREFTA
                     NVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISHEQQ
                     MRFSQ"
     gene            34907..35770
                     /locus_tag="OO349_001358"
     CDS             34907..35770
                     /locus_tag="OO349_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HCR5866496.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSIYLLAERGKLAELRLLMIPGQ
                     VDYLQHIEELAAFIKGLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(35984..36766)
                     /locus_tag="OO349_001359"
     CDS             complement(35984..36766)
                     /locus_tag="OO349_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR5866497.1"
                     /translation="MICRFIDTHCHFDFPPFIGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAENYQPLYAALGLHPGMLEKHSDVSLEQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKHHDLPRAGVVHGFS
                     GSQQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFAVLCELRPEPADEIAEVLLNNTYAVFNVRG"
     gene            complement(36763..37836)
                     /locus_tag="OO349_001360"
     CDS             complement(36763..37836)
                     /locus_tag="OO349_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HCR5866498.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYEPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRISEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(37958..38119)
                     /locus_tag="OO349_001361"
     CDS             complement(37958..38119)
                     /locus_tag="OO349_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HCR5866499.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(38236..38841)
                     /gene="osmY"
                     /locus_tag="OO349_001362"
     CDS             complement(38236..38841)
                     /gene="osmY"
                     /locus_tag="OO349_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HCR5866500.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRKVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(39234..40823)
                     /gene="prfC"
                     /locus_tag="OO349_001363"
     CDS             complement(39234..40823)
                     /gene="prfC"
                     /locus_tag="OO349_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HCR5866501.1"
                     /translation="MTLSPYLKEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLNGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(40914..41591)
                     /gene="yjjG"
                     /locus_tag="OO349_001364"
     CDS             complement(40914..41591)
                     /gene="yjjG"
                     /locus_tag="OO349_001364"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HCR5866502.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRSNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(41606..42052)
                     /gene="rimI"
                     /locus_tag="OO349_001365"
     CDS             complement(41606..42052)
                     /gene="rimI"
                     /locus_tag="OO349_001365"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HCR5866503.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHSFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(42021..42434)
                     /gene="holD"
                     /locus_tag="OO349_001366"
     CDS             complement(42021..42434)
                     /gene="holD"
                     /locus_tag="OO349_001366"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710111.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HCR5866504.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIASPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            42537..43568
                     /gene="rsmC"
                     /locus_tag="OO349_001367"
     CDS             42537..43568
                     /gene="rsmC"
                     /locus_tag="OO349_001367"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HCR5866505.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSCQMGDNARFSLVATANDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGCFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            43845..43931
                     /locus_tag="OO349_001368"
     tRNA            43845..43931
                     /locus_tag="OO349_001368"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43879..43881,aa:Leu,seq:cag)
     gene            43960..44046
                     /locus_tag="OO349_001369"
     tRNA            43960..44046
                     /locus_tag="OO349_001369"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43994..43996,aa:Leu,seq:cag)
     gene            44081..44167
                     /locus_tag="OO349_001370"
     tRNA            44081..44167
                     /locus_tag="OO349_001370"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:44115..44117,aa:Leu,seq:cag)
     gene            complement(44206..44442)
                     /locus_tag="OO349_001371"
     CDS             complement(44206..44442)
                     /locus_tag="OO349_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HCR5866506.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYILLPSCLALIAGIMLMIMNLNQG"
     gene            44583..45371
                     /gene="fhuF"
                     /locus_tag="OO349_001372"
     CDS             44583..45371
                     /gene="fhuF"
                     /locus_tag="OO349_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HCR5866507.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRAMIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGYCTLK"
     gene            complement(45409..46086)
                     /gene="bglJ"
                     /locus_tag="OO349_001373"
     CDS             complement(45409..46086)
                     /gene="bglJ"
                     /locus_tag="OO349_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710107.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HCR5866508.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLSRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIVDDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(46044..>46610)
                     /locus_tag="OO349_001374"
     CDS             complement(46044..>46610)
                     /locus_tag="OO349_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR5866509.1"
                     /translation="LVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQA
                     VELLMCPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILT
                     LSERKVLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQG
                     ARELNLPSVYGDAAEWNTAELRREMSHS"
BASE COUNT        11045 a        12697 c        11729 g        11139 t
ORIGIN      
        1 tcggggggat ttttttattc gctaacgtcg cgtcttgcac tcttcggtct gaatgcagca
       61 accacttctg gtgccgtttc aacgtatggc ccttccagca actgaatgca gtacggtacg
      121 ctggcaaaaa taccatgcac cacaacgtta ccctcagcgt ccttcacacc ttccagcgtc
      181 tctttaatag acttcggctg accgggtaag ttaaggatca gcgcctgttt gcgaatcacg
      241 cccacctgac gcgaaaggat cgcagttggt acaaaatgca ggctgatctg gcgcatctgt
      301 tcaccaaagc caggcatctc gcggtccgct actgccagcg tcgcatcggg cgttacgtca
      361 cgacgcgccg ggccagttcc gcccgtggtg agcaccagat ggcaactcat ttcatccacc
      421 agctcacaca acgtttgctc gatgatcgcc tgctcatcgg ggattaagcg ggtttccagt
      481 tcaaacggcg tggttagcgc cgatgtcagc cattcttcca gcgctggaat gcctttatcc
      541 tgataaacgc cgctggatgc gcgatcagag atggaaacta agccaatacg taaagtattc
      601 atcaagattt ttccgtttaa acaatctcgt taaacagaat gatacactgc gaagggagtg
      661 acagacaggc ttcagaagag gtagcgtgac cgacttcccg gtcacgctaa gaatgattac
      721 agcagatcgc cgatcatttt ttccagtttt tcctggtcaa tagcaaactt acggatacct
      781 tccgccagtt tatctactgc cattggatcc tggttgtgct gccacaggaa ctcggactca
      841 gtgatacgcg ccggacgcgc tttcacttcg ccggtgtaag acagtttacg ttcgatagcc
      901 ccttcgctct ccgccagctc tttcagcagt gccggtgcga tggtcagacg gtcacagcct
      961 gccagttcca gaatttcgcc gatgttacgg aagcttgcgc ccataaccac ggtttcataa
     1021 ccgtgctctt tgtagtactg gtagatttca gatacagaaa ccacgcccgg atcttctgcc
     1081 ggagcgtact ctttcttatc ggtattcgct ttgtaccagt caagaatacg gccaacaaac
     1141 ggcgagatca ggaacacgcc cgcctccgca caagcacgag cctgagcgaa ggagaacagc
     1201 agggtcaggt tacagttgat accttctttt tccagctgtt ctgcagcacg gataccctgc
     1261 caggtagaag ccagtttgat cagaatacga tcgttgctaa tacctgcatc gttgtagagt
     1321 ttgatcaggc gttttgcttt cgcaattgac gcttcggtgt cataggaaag acgcgcatca
     1381 acttcagttg agatacggcc cggaaccagt ttcaggattt ccagaccaat atttactgcc
     1441 agtttgtcgg tcgcgtccac gatctgctgc gcgcgatcgt tgctctgctg tttcgcccag
     1501 gcgacagcat catcaatcaa cttacggtat tccggaatct gcgctgcgtt aagaatgaga
     1561 gaagggttgg ttgtggcatc ctgcggttga tacagcttca ttgccgcgat gtccccagtg
     1621 tcggccacta cggtggtgta ctgacgaagg gaggtcaatt tgtccgtcat gatagtattt
     1681 ctctttaaac agcttgttag ggggatgtaa ccggtctgcc ctgatgatat cacgacgcaa
     1741 cgtcagcgca accgccgaca agacggttat cgccaggcga gactgtttcg gatttctgac
     1801 actgcgttga tatcgcccac catttttata caaaacctca tgtatgctac gcagaagtta
     1861 tcaagtacct cgtagcgtat atacttctta aacaattggt aacgtttaca caggaaagtc
     1921 atcgcgaccg gcaataagag ggatatgcat gccagatttt ttctccttta ttaacagcgt
     1981 cctttgggga tcggtaatga tttacctgct cttcggcgca ggttgttggt tcacttttcg
     2041 caccggattt gtgcagtttc gctacatccg ccagtttggc aaaagtctta aaaatagcat
     2101 tcatccacag ccaggcggtt taacctcatt tcagtcattg tgtaccagtc ttgcggcgcg
     2161 cgtgggtagc ggcaacctgg ccggcgttgc gctggcaatt accgccggtg gaccaggtgc
     2221 cgtcttttgg atgtgggttg ctgcgtttat cggcatggcg acctcgtttg ccgaatgttc
     2281 ccttgcacaa ctttataaag aacgtgacgt caatgggcag tttcgtggcg gaccggcatg
     2341 gtatatggcg cgcgggctgg ggatgcgctg gatgggcgtt ctgttcgccg tctttttgct
     2401 catcgcctac ggcataattt tcagcggagt tcaggcgaac gccgttgccc gagccctgag
     2461 tttttctttt gattttcctc cgctggtgac gggcattatt ctcgctgtct ttgctctgct
     2521 ggcaatcact cgcggtcttc atggcgtcgc ccggctcatg caggggtttg tcccgttgat
     2581 ggcgataatc tgggtactga ccagcctggt gatttgcgta ataaatatcg ggcaacttcc
     2641 ccacgtcatt tggtctattt ttgagagtgc ttttggctgg caggaagcgg caggcggcgc
     2701 ggcgggatat accttaagcc aggcgattac taacggtttt cagcgcagta tgttttccaa
     2761 tgaggtggga atgggatcga cgccaaacgc ggcagcggca gctgcatcct ggcctccgca
     2821 tccggcagca caagggattg tccagatgat tggcattttt atcgacaccc tggtcatctg
     2881 tacggcaagc gccatgctga tattactggc gggtaacggc acaacctaca tgccgctgga
     2941 aggtattcag cttatccaga aggcgatgcg ggtgctaatg ggttcctagg gtgctgaatt
     3001 tgttaccctc gtggttattc tgtttgcctt cagctccatc gttgccaact acatttatgc
     3061 cgaaaacaat ctcttctttt tacgcctgaa caaccctaaa gcgatctggt gtttgcggat
     3121 ctgcaccttc gcaacgatca tcggcggcac cttgctaagc cttccgctga tgtggcaact
     3181 ggcagatata taatggcctg catggctatt accaatttga ccgccatttt actgctctcg
     3241 cctgtggttc ataccattgc cagtgattat ctacgccagc gtaaactcgg cgtgcgcccg
     3301 gtgtttgatc cgttgcgtta tccggatatc ggtcgccaac tttctccgga cgcgtgggat
     3361 gatgtttcgc aggagtaatc acaactatcg atcaactcat tctctttttt tgctaaagtc
     3421 ggcataaatt tcctgcaagg actggatatg ctgattctta tttcacctgc gaaaacgctt
     3481 gattaccaaa gcccgttgac caccacgcgc tatacgctgc cggagctgtt agacaattcc
     3541 cagcagttga tccatgaagc gcggaaactg acgcctccgc agattagcac gctgatgcgc
     3601 atcagcgaca aactggcggg tatcaacgcc gctcgctttc atgactggca gccagatttc
     3661 acgccggcga atgcccgcca ggcgattctg gcgtttaaag gtgatgtcta caccggcttg
     3721 caggccgaga ccttcagcga agacgatttc gattttgccc aacagcattt gcgaatgctt
     3781 tccggcttgt atggcgtact gcgcccgctc gatttaatgc agccttatcg tctggaaatg
     3841 gggatccgtc ttgagaatgc ccgagggaaa gatctgtatc aattctgggg agatatcatc
     3901 accaacaagc tgaacgaggc gctcgcagca caaggcgata atgtggtgat taatctggcg
     3961 tcagatgagt attttaaatc ggtgaagccg aagaaattga atgccgagat tatcaagccg
     4021 gtgttcctcg atgagaagaa cggcaaattt aagatcatca gcttctacgc taagaaagca
     4081 cgcggtctga tgagtcgttt cattattgaa aatcggctga ccaaaccaga gcaactgact
     4141 ggttttaata gcgaaggtta cttctttgat gaagattcct ccagcaatgg cgaactggtg
     4201 tttaaacgct acgagcagcg ttaagtttgt agtcaatgcc ggatgcggcg tgaacgcctt
     4261 atccggccta caaaagcatg cagattcaat atattgcaga attaacgtag gcctgataag
     4321 acgcgccagc gtcgcatcag gcgctgaatg ccggattgtt acccggcatt tgtcatttag
     4381 cggtgatgtt tgcctggacc atgaccgccg tgatgatcat gaggagcttt cttatggtgg
     4441 cgcggcggtg acggcggtcc gtgtgggtgc cagcgattgc ctcgccattc ataatgttgt
     4501 ttccaccagc cgtggtcgcg ccagtgacct ccatcccagt aatagccacg attatcacga
     4561 tcgcctatct gtaattttac tgacgggact aacgtaattt ccgcagcctg tgctgccatg
     4621 ggagcgacca gaaccaggga aagtgcgagt acgatagatt gcatcttttt cacaggttac
     4681 tccttttatc attgcccgtt atgggtcgat atccggagat tacgcgatga gaacgacggt
     4741 tgttattctc cgcaatccta atctctaagt taccgcattt attgataatt tctccttttt
     4801 ccctgtcgtt tttctccata atttcttcat aaaaaagccg ggctgcataa aagcaaaccc
     4861 ggcctgattg agataatgaa tagattttac tgatgattca tcatcaattt acgcaacgca
     4921 gaaaaatcgg cgggcagatt atgtgaaagc aagggtaaat cagcacgttc tgccagctct
     4981 tttggcagat ccaacgtttc accgagaatc gcttccacgc tctctttaaa tttcgccgga
     5041 tgcgcggtgc cgaggaacaa gccatattcg cctggattca actggtcacg cagcgcacga
     5101 tacgctacgg cagcgtgcgg ctccgaggtg tagcccagtt cttttaactc acgcattgtc
     5161 tgttgcgtgg tttcatcatc cacggctgca taacccagct ctttcagttg ccagattttg
     5221 cggcggaaca actcttccac acgcggccag ttgttcggct gactcacgtc catcgcgttg
     5281 gataacgtcg cctgagtcgc tttgggtgac cactgaccgt cgtgcaggaa acgtggcacg
     5341 gtatcgttca cgttggtcgc agcaataaaa cgtttcaccg gcagaccgaa tgacttcgcc
     5401 agcagacccg ccgtcaaatc gccgaagttt ccgcttggca ccgagacaac cagctggttg
     5461 cgcgcctcct gcggcagctg cgcaacagct tcaaagtagt agcaaatctg cgccagcaaa
     5521 cggctgatgt taatcgagtt agccgagttt aaccccagcg ccactttcag ttcttcatca
     5581 tcaaacgcct gcttcaccag cgcctgacag gcatcgaaat cgccgtcgat ggcaacagtt
     5641 tcgatattgc cgcccaacgt acagaacagt ttttcttgca gtggactgat tttgcctcgt
     5701 ggatagagga taaccacttt cacattcggc aaaccgtaga aagcatgagc cactgcagct
     5761 ccggtatcac cggaggtcgc ggtcagaatg gtcactggct tatcatccgc aatatgggtc
     5821 agcatttgtg ccataaagcg accgccgaaa tctttaaatg ccagcgttgg cccgtggaac
     5881 aattccagac aaccgacatc gctttcaaca ttggcgaccg gagccgggaa ggcaaacgcc
     5941 gcgcgcacgc gctcttccag gatttcctgt gggatttcat caccaataaa cgccgagagg
     6001 atcttcgcac tgcgggtgac aaaatccagc ttcagcatct catcaatttc agtcaggctg
     6061 aattccggca ggtcgtgcgg aaaaaacagc ccctgatttt tgcccaaccc ctgggttacg
     6121 gcttgcgcaa agctgacctg ctcgttgtga tctttcagat tgtagagttt catttagttt
     6181 tccagtactc gtgcgcccgc cgtatccagc cggcaaatat gaacaaaacc ttcctgattt
     6241 tgcaggtagt tcttacccaa ccagtcggca acgcgctggg cggtatccgg cttgtcacat
     6301 agagcgaaca aggtcgggcc ggagccggag ataccgctca ctaccgcgcc gatttccgcg
     6361 accgcctgcc gcgcctgccg gaagccaggc agtaaccgtt cacggtaggg ttcagcgata
     6421 acatctttca tcagcttcgc ggcaagctca ggctgacggg aatagcaggc gtgaatgaag
     6481 ccagccagat gtcgcccgtg cgcaatgcaa tcctggcggc gatactgcgc cggtaaaata
     6541 gcccgggctt ctgccgtcga gactttaatt cccggatacg ccagcaccca cagccactca
     6601 tcaaaccctg gcacttgctg gctgatgatg tcgttttctt cgatcatcaa ctgcatacca
     6661 ccaagaaaac acggtgccac gttgtcgtaa tgaatgctgc cggagatacg tccttccagc
     6721 tcgcccatca aagccagcaa acgagtgtca ttaagcggct tgccgcagtg ttcattcatc
     6781 gccatcagcg ccgcgaccac cgaacaggcg ctggagccta agcccgaacc gatcggcata
     6841 ttcttttcca gagtcatcgc cactggaatt tgcttgccaa gctcctggca aaaacgctcc
     6901 cagcactgat aaacgatatt ttcccgtggt tctgacggca gcttatcggc aaagcgtccg
     6961 aggttgttga gactgaatgt ctctgccgcc tcaaccgtga ctacatctcc gagcaatgca
     7021 ccatcaacgg gtgccaccgc cgccccgagc acatcaaacc cgacgctcat attggcactg
     7081 gaagccgggg cataaacttt aaccatgtca gactcctaac ttccatgaga gggtacgtag
     7141 cagatcggca aagacaccgg cagcggtaac gtcattgccc gcaccatatc cgcgcagcac
     7201 caacggcagc ggctgataat agtggctata aaaggccagg gcgttttcgc catttttcac
     7261 tttgaacagc ggatcattcc catccacttc ggcaatcttc acgcggcaga caccatcttc
     7321 atcaatattg ccaacatagc gcaaaacttt tccttcatca cgggccttcg ccacgcgcgc
     7381 ggcaaagaga tcgtcgagct gtgacagatt cgccataaaa gcggcaacat caccctcggc
     7441 gttaaactct gcgggcagca caggttcaat ttcaatatcc gccagctcca gttcacgtcc
     7501 cgtttcacga gcgagaatca atagtttacg cgccacatcc ataccagaaa gatcatctcg
     7561 cgggtccggt tcggtataac ccatttcccg cgccagcgtg gtcgcctcgg agaaactcat
     7621 gccttcgtct aacttgccga agatataaga aagcgaacct gaaagaatgc cggagaactt
     7681 caccaattca tcacctgcat tgagcagatt ttgcaggttc tcaataaccg gtaatccagc
     7741 cccaacgttg gtgtcataga ggaatttacg ccgcgatttt tccgccgcat aacgcaactg
     7801 atgatagtaa tccatcgacg aggtgttggc ctttttgttc ggcgtaacaa cgtggaaacc
     7861 ttcgcgcagg aagtcggcat attgatccgc cactgcctgg ctggaggtgc agtcaacaat
     7921 gaccgggttc agcagatgat attctttcac gaggcgaatt aagcgcccga gattaaacgg
     7981 ctctttggct tgtgcaagtt cttcctgcca gttttccaga ttaaggccat gcacactagt
     8041 gagcagagcc ttcgagttgg caacgccgca gacacgtaag tcgatatgtt tattcttcag
     8101 ccagctttgc tgacgcttca gttgctccag cagcgcaccg ccaacgccac cgacgccaat
     8161 cacaaacact tcgataacct gatcggtatt gaacagcatc tgatgagtaa cgcgcacgcc
     8221 agtggtcgca tcatcgttat ttaccacgac agagattgag cgttcagaag atccctgagc
     8281 aatggcgaca atgttgatat tggcgcgggc cagcgcggca aagaatttcg ccgagatccc
     8341 acgcaaggtg cgcataccat cacctaccac cgagataatg gccagccgtt ccgtcaccgc
     8401 cagcggctcc agtaagcctt ctttcagttc caggtagaac tcttcctgca ttgcccgttc
     8461 agctcgcaca cagtcgcttt gtggaacgca gaaactgata ctgtattcgg aagatgattg
     8521 cgtaatcagc accacggaaa tacgggcgcg tgacattgca gcaaagacgc gcgccgccat
     8581 gccaaccatc cctttcatcc ccgggccgga aacgctgaac attgccatgt tattcagatt
     8641 ggaaatgccc ttgaccggta attcgtcttc atcacggctg gcaccaatga gcgtacctgg
     8701 tgcttgagga tttccggtat ttttaatcag gcaagggatc tggaactggg cgatgggggt
     8761 aatggtgcgg gggtgaagaa ctttagcgcc gaagtaggaa agctccatcg cttcctggta
     8821 ggacatcgac ttcaacaacc tcgcatcggg cacctgacgc gggtcgcagg tatagacccc
     8881 gtcaacgtcc gtccaaatct cgcaacaatc ggcgcgtaaa caggcagcca gcaccgccgc
     8941 ggagtagtcg gaaccgttac gtccaagcac caccagttcg cctttttcat taccggcggt
     9001 gaaacctgcc atcaacacca tgtgatcagc cggaatgcgg cttgccgcaa tacggcgggt
     9061 ggactcagca atatcgacgg tagattcgag gtaatgcccc actgccagca gtttttcgac
     9121 cggatcgata acggtaacgt tgtgaccacg cgcttctaac acgccggcca taatggcgat
     9181 cgacattttc tcgccacggc aaatcagcgc agcgttgatg ctatccgggc actgccccaa
     9241 caaactaatg ccatgcagga catgttttat ttgggcaaat tcctgatcga cgaaagtttt
     9301 caattgcgcc agcgggaacc ccggctgggc ggcggcgagt cccgtcaaaa gttcggcaaa
     9361 aatacgttcg gcatcgctga tattgggtaa agcatcctgg ccgctaatgg ttttttcaat
     9421 catcgccacc aggtggttgg tgattttggc gggggcagag aggacggtgg ccacctgccc
     9481 ctgcctggca ttgctttcca gaatatcggc aacacgcaga aaacgttctg catttgccac
     9541 tgatgtaccg ccgaacttca acactcgcat ggttgttacc tcgttacctt tggtcgaaaa
     9601 aaaagcccgc actgtcaggt gcgggctttt ttctgtgttt cctgtacgcg tcagcccgca
     9661 ccgttacctg tggtaatggt gatggtggtg gtaatggtgg tgctaatgcg tttcatggat
     9721 gttgtgtact ctgtaatttt tatctgtctg tgcgctatgc ctatattggt taaagtattt
     9781 agtgacctaa gtcaataaaa ttttaattta ctcacggcag gtaaccagtt cagaagctgc
     9841 tatcagacac tcttttttta atccacacag agacatattg cccgttgcag tcagaatgaa
     9901 aagctgaaaa atacttacta aggcgttttt tatttggtga tatttttttc aatatcatgc
     9961 agcaaacggt gcaacattgc cgtgtctcgt tgctctaaaa gccccaggcg ttgctgtaac
    10021 cagtcgacca gttttatgtc atctgccact gccagagccg tcagcaatgc catgactcgt
    10081 tcgcgtaaag cttgcagttg atgttggtct gccgttgtgt cacttttcgc cggttgttgt
    10141 attaatgttg ctaattgata gcaatagacc atcaccgcct gccccagatt gagcgaagga
    10201 taatccgcca ccatcggcac accagtaagc acgtcagcca acgctaactc ttcgttagtc
    10261 agcccggaat cttcgcgacc aaacaccagc gcggcatggc tcatccatga agatttttcc
    10321 tctaacagcg gcaccagttc tactggcgtg gcgtagtaat gatatttcgc ccgactacgc
    10381 gcagtggtgg cgacagtgaa atcgacatcg tgtaacgatt cagccagtgt cgggaaaact
    10441 ttaatattat caataatatc accagatcca tgtgcgaccc agcgggtggc tggctccagg
    10501 tgtgcctgac tatcgacaat ccgcagttcg ctaaaaccca tcgttttcat tgcccgcgcc
    10561 gctgccccaa tattttctgc tctggcgggt gcgaccagga taatcgttat acgcatattg
    10621 ccactcttct tgaccaaata accgcgaaca gggtgatcac tgtcaactta ttacgcggtg
    10681 cgaatttaca aattcttaac gtaagccgca gaaaaagccc tttacttagc ttaaaaaagg
    10741 ctaaactatt ttctgactgc actaacggtt taattgttaa aaaatgctac atatccttct
    10801 gtttacttag gataatttta taaaaaataa atctcgacaa ttggattcac cacgtttatt
    10861 agttgtatga tgcaactagt tggattatta aaataatgtg acgaaagcta gcatttagat
    10921 acgatgattt catcaaactg ttaacgtgct acaattgaac ttgatatatg tcaacgaagc
    10981 gtagttttat tgggtgtccg gcccctctta gcctgttatg ttgctgttaa aatggttagg
    11041 atgacagccg tttttgacac tgtcgggtcc tgagggaaag tacccacgac caagctaatg
    11101 atgttgttga cgttgatgga aagtgcatca agaacgcaat tacgtacttt agtcatgtta
    11161 cgccgatcat gttaatttgc agcatgcatc aggcaggtca gggacttttg tacttcctgt
    11221 ttcgatttag ttggcaattt aggtagcaaa catgcagacc ccgcacattc ttatcgttga
    11281 agacgagttg gtaacacgca acacgttgaa aagtattttc gaagcggaag gctatgatgt
    11341 tttcgaagcg acagatggcg cggaaatgca tcagatcctc tctgaatatg acatcaacct
    11401 ggtgatcatg gatatcaatc tgccgggtaa gaacggtctt ctgttagcgc gtgaactgcg
    11461 cgagcaggcg aatgttgcgt tgatgttcct gactggccgt gacaacgaag tcgataaaat
    11521 tctcggcctc gaaatcggtg cagatgacta catcaccaaa ccgttcaacc cgcgtgaact
    11581 gacgattcgt gcacgcaacc tgctgtcccg taccatgaat ctgggtactg tcagcgaaga
    11641 acgtcgtagc gttgaaagct acaagttcaa tggttgggaa ctggacatca acagccgttc
    11701 gttgatcggc cctgatggcg agcagtacaa gctgccgcgc agcgagttcc gcgccatgct
    11761 tcacttctgt gaaaacccag gcaaaattca gtctcgtgct gaactgctga agaaaatgac
    11821 cggccgtgag ctgaaaccgc acgaccgtac tgtagacgtg acgatccgcc gtattcgtaa
    11881 acatttcgaa tctacgccgg atacgccgga aatcatcgcc accattcacg gtgaaggtta
    11941 tcgcttctgc ggtgatctgg aagattaatc ggctttacca ccgtcaaaaa aacggcgctt
    12001 tttagcgccg tttttatttt tcaaccttat ttccagatac gtaattcatc gtccgttgta
    12061 acttctttac tggctttcat tttcggcagt gaaaacgcat accagtctat attacgagtc
    12121 acaaacatca tgccggccag cgccaccacc agcacactgg ttcccaacaa cagcgcgcta
    12181 tcggcagagt tgagcagtcc ccacatcact ccatccagca acaacagcgc gaggataaac
    12241 aacatgctat tgcgccagcc tttcaatacc gcctgcaaat aaataccgtt cattaacgcc
    12301 ccaatcagac tggcgattat ccatgccacg gtaaaaccga tatgttcaga aagcgccagc
    12361 aagagcaaat aaaacatcac caatgaaagc cccaccagca aatattgcat tgggtgtaaa
    12421 cgttgcgcgg tgagcgtttc aaaaacaaag aacgccataa aagtcagtgc aatcagcaga
    12481 atggcgtact tagtcgcccg gtccgttaat tggtattgat cggctggcgt cgtcactgcg
    12541 acgctaaacg cggggaagtt ttcccagccg gtatcattgc ctgaagcaaa acgctcaccg
    12601 agattattag caaaccagct gctttgccat tgcgcctgaa aacctgactc gctagcttcc
    12661 cgtttggctg gtagaaaatc acctaaaaaa ctgggatgcg gccagttgct ggttaaggtc
    12721 atttcgctat tacgcccggc aggcaccaca gaaagatcgc cggtaccgct taaattcagt
    12781 gccatattca gctgcaggtt ctgcttacgc cagtcccctt cgggtaaagg gatatgcacg
    12841 ccctgcccgc cttgctctaa cccggtaccg ggttcaatgg tcagtgccgt tccgttaact
    12901 tcaggcgctt tcaccacacc aataccacgc gcatccccga cgctaatcac aataaagggc
    12961 ttgcctaaga tgatatttgg cgcgttgagc tcgctaagac gcgaaacatc gaaatcggct
    13021 tttagcgtta aatcactgtg ccagacctga ccggtataaa tccctatctt gcgttcttcc
    13081 acgttctgat tgccatcaac catcaatgac tcaggtaaca aaaaatggat aaaacttcgt
    13141 ttccgctcca cggttttatc ctcttcctgc accgtataaa gctcggtcac aggaatagcg
    13201 ataagcggcc caacgagttt ttgcggcccg ctggtacttt ggcgaatcgc atcttccaca
    13261 tcgctacggt aatcggcacg ttcgacaatc acttgccgaa tcagcattat cggaatcaac
    13321 aacagcaata ctgcaccaaa caaggtggtc attttccaga acagggggga tttcaacata
    13381 gtcttctcct ttgcaatggc agcaggatac gaaggctatg tgggaagaat ttgaagctat
    13441 gtgaagtgac ggtgaagtcg tagcgaggcc agcacgccac cttcctgcac gttgcgcagc
    13501 gtgacttcgc cgttaaacaa acgggcgacc tcactgacga acgccaaccc cagaccgctg
    13561 cttttttgcc catttgcacg cggcaaagag taaaagcgtt caaaaatacg tgacagcgcg
    13621 tagtcaggaa taccactacc ggtatccagc accttaagcg tgacgtgttc ctgatccact
    13681 tcggcgctta gcgttatgca accgctctcg ggagtaaaat cgatggcgtt atccagtaaa
    13741 ttccccagcg cctgatccag taacgccggt tcagcagcaa cattaacctc ggtgggcata
    13801 acatgcaaag tgatgttttt ttctgccaac tgcacggtgc gcgcttcgct gacgcggcga
    13861 aataatgccg ccacatcaac agcagtcaga acgacttcct gacgattctc cagtcttgcc
    13921 tggcgtagta acgtttccac cagcgcctgc attcgcgcat tttgtgtcag aatgttgtca
    13981 gtaaaacgag ccactacttc aggcggcgga ccttcgcgta aaatttccgc cgcgccacga
    14041 atcgccgcca gtgggctttt tagctcatgg gttaacgcat aaacatactg ctcaatatag
    14101 tttttccctt ccagcttcac gcgcatactt tccagcgcct gcgcgagttt acgcagctcg
    14161 ctactaccga gatcgggaag aggaacgggc ttattgtcag tgacggaatc agcatagcga
    14221 gtgagccggg caatggagcg gttgatccac caaaccatgc ctgcgccaat caccagtgca
    14281 atccccaaca aaatggcgct ggcccataaa attcgccgct cgctgcgttt aatgaccgga
    14341 gccatcgccg cgttcggttt gcctacgctc aaaacgccaa taagccgcga gccgcccata
    14401 atcggcgcgg caacatacat caccgaactt tcgggatcgg caggattttg caacgtgctg
    14461 cgcgcaccat actgaccacg caacgttagc cagacgtcat tccagcgcga ataatcctgt
    14521 ccaacggctt tatttgccga atcgaacaat actttgccat gcgcatcggt catatagaca
    14581 tgatattcat tgcgcacttt gttaatgcca ccaatattgg cgcgaaacgg gcgatgttgt
    14641 agctgattaa acgcctgcgc cagttgccca tgcgttgggt ccccagagag caaatcggga
    14701 cgcgccagct ccgcaagtaa cgttgccgtg tcgatcaacg tcccctccgt tgctcttcgc
    14761 acgcccggtt taacttcttt gacaaaaata gccagtacga accaggccgc caccgccacc
    14821 agtaaaaaat agcccagcag caaccgcatg ccgatacgca ttacaagccc ctcaggctat
    14881 atcccatgcc gcgatgagta ttaatcggtg aaagatcggg gttgatggcg cgcagcttgg
    14941 cacgcagcgt tttaatgtgg atatcgacgg tgcgatcgta ggtgtcctgc gcatcttccc
    15001 acacgctatc cattagttgc tggcgggacc agacgcggcc tggcgacttg agtaacgtct
    15061 tcagcaataa aaactcatac cgcgtcagcg ttaatggcgt gtcaaaccag ctgatctgcg
    15121 ccgcgggttc attcaattca aaatgaccaa tacggatgac gggagacggc gtcgagaact
    15181 tcttcacccg acgcagtaag gtgcgcaccc tggcgcacac ttcgcggggt gaaaacggtt
    15241 tagccacata gtcgtcagcg ccaatttcca gcccaagcag gcgatcgacc tcttcacttc
    15301 gggccgtcag gaacagtaca ggtaacgccg gatggagcgc cagtaactgg cggcacaatt
    15361 caaagccact gatatcaggc aaaccaacgt cgagaatcat gacgtcggga acctgctggc
    15421 gagctttatc cagcaccggc aaacctcgct caaagacctc gacggcaaaa ccttcctgct
    15481 gcaacatgta gaccagcgtg tcggctatcc cttgctcatc ttccactaac cagaccgttt
    15541 cccgttgcat aaatcgcctc tgttattgcc gccacggcat gacaggaacc gcgctaatcg
    15601 cgtttttcgg cgaaccttct acaactttgt cggagtaagc cagataagcg agcgcgttgc
    15661 gtttggcgtc ataaaagcgc acgacctgta acgacttaaa gaccagggac gtgcgttttt
    15721 tgaatactac ctcgccctga gctttgccgt ttttaatacg atccgacagt tcaatcggcc
    15781 cgacttgctg acaagaaatg gccgcatcgg aggtatcttc tgccaggccc aatccccctt
    15841 taataccacc ggttttcgcc cggctcacat aacaggtgac atttttcaca tcgggatcgt
    15901 caaaggcttc cacaacaatt ttgtgatccg ggccgatcat tttaaatacg gtgtcgaccg
    15961 aaccaatctc ttctgcatga gccaatggcc ccagcattat caggcttaaa gacaggatca
    16021 aatgcttgta tttcatattg ttaccatttt tatctattac acgagcaatt agttcgtcac
    16081 ggtaaataac atattgatta agaacaaaat ctcaattctt ttatttccgt caagccctaa
    16141 aacatactct actaagaaaa aaacactgaa tgctaaaaca gcaaaaaatg ctattatcca
    16201 attacctgat gtcaggtgct cgttgttgaa aggatgagga tattttatgg atcaggccgg
    16261 cattattcgc gaccttttaa tctggctgga aggtcatctg gatcagcccc tgtcgctcga
    16321 caatgtagcg gcgaaagcag gttattccaa gtggcactta cagagaatgt ttaaagatgt
    16381 cactggccat gctattggcg cgtatattcg tgctcgtcgt ttgtcgaaat cggcggtcgc
    16441 actacgcctg actgcgcgtc cgattctgga catcgcgctg caataccgct tcgactctca
    16501 acagacattt acccgcgcat tcaagaagca gtttgcccag actcctgcac tttaccgccg
    16561 ttctcctgaa tggagcgcct ttggtattcg cccgccgcta cgtctgggtg aattcacaat
    16621 gccagagcac aaatttgtca ccctggaaga tacgccgctg attggtgtta cccagagcta
    16681 ctcctgttcg ctggagcaaa tctctgattt ccgccatgaa atgcgttatc agttctggca
    16741 cgattttctc ggcaacgcgc cgaccattcc gccggtgctc tacggcctga atgaaacgcg
    16801 tccgagtcag gataaagacg acgaacaaga ggtattctat accaccgcgt tagcccagga
    16861 tcaggcagat ggctatgtac tgacggggca tccggtgatg ctgcagggcg gcgaatatgt
    16921 gatgtttacc tatgaaggtc tgggaaccgg cgtgcaggag tttatcctga cggtatacgg
    16981 aacgtgcatg ccaatgctca atctgacgcg ccgtaaaggt caggatattg agcgatacta
    17041 cccggcagaa gacgccaaag cgggagatcg cccaattaat ctgcgctgtg aactgctgat
    17101 tcctatccgc cgttaacgct gcagctcatc taatgcaggg gcgtccagat gcgagatgtc
    17161 ccctgcggtt tcaacgaccc agccagacgc cagccacagg ctttcctgat aatccacgcg
    17221 cgaaatagaa cagttacgca gacgtaagcg gcgctctacc catgctggta atccgagaat
    17281 cgtactcacc agacatccca gtgcaatacc atggctcacc agcagcggtc ggcttccctg
    17341 cggtaagtcc cggcaagatt ccagtgccgc attgacgcga tcgctgagct cctgcattga
    17401 ctcgccttca ggaatacgcc cgtcaacggt gccattgacc agctgccgac gccagttctc
    17461 ttcttcttcg gtcagagaat cgatatgtct tttttccagc acacccatgt ttaattcacg
    17521 caggcgagaa tcaaagatga tgtcacagcc gcaggcctgg gcgatgattt ccgccgtacg
    17581 ccgggtgcgt cctaaatcgc tactgataat atgcgtaatg ccaagctctt tggcacgggt
    17641 tgccacctgc atcgcctgtt gctcaccttt ggcggtcagc gggctgtcag actggccctg
    17701 aatacgtcgc tcggcgttcc actgcgtttc accgtggcgg actaggtata cctgtaacat
    17761 gctttttttc cgtatactgc tgtaacgtta attttctgag taatgctgat tatgcaccaa
    17821 gttgtctgtg cgaccaccaa tcccgctaaa attcaggcca ttctgcaggc atttcacgag
    17881 atcttcggcg aaggatcctg ccatattgca tccgttgccg tcgagagcgg tgtaccggaa
    17941 caaccctttg gcagtgagga aacgcgcgct ggcgcacgaa atcgggtagc aaatgcccgc
    18001 cgtttacttc cagaggctga tttttgggtg gcgattgaag ctggcattga tggcgacagc
    18061 actttcagtt gggtggtgat tgaaaacgcc agccagcgcg gcgaggcgcg ttcggcaact
    18121 ttgccgttac ctgcggtgat tctggaaaaa gttcgtgaag gcgaggcgct tggtcccgtg
    18181 agtcgcgtta taccggtatt gatgagattg gtcgtaagga aggggcgatc ggcgtgttta
    18241 ccgccgggaa actcactcgc gccagcgtct atcaccaggc ggtgatcctg gcgctgagtc
    18301 cgtttcacaa tgccgtgtat caggcattgt aggacggata aggcgttcac gccgcatccg
    18361 acattcggtg cacgatgcct gatgcgacgc tgccgcgtct tatcaggcct acaaaatcaa
    18421 tcgcttttca gcaacacctc ttccagccac tgacgcagct ccacgggcgc ggctttcagg
    18481 ctgttagatc cacgcgtaat cgtcgcgatg cccgcgccga gttcattttt taactcacgc
    18541 tgactcattt cgccacgcaa cagctcttcg acaatacgca cgcgagtccc caacgcttcg
    18601 cgctcatctg gcgtcagcat caggtttaac aacggtaaat ggagatcgtt ttggtaggca
    18661 ttcttaagca ggtcgacaaa acgtaaccac tcctggtgac gctgttctgc catcgctgct
    18721 gaatagggtg attgttgggc cataatatgt cgccattgtt agcgggggaa gcaaaatgac
    18781 tcccccggtt gtactcgcta aagagtacga tagcatatca taaacgtgcg gatcagtaac
    18841 gacgtcccca ttccgtggcg ctcatcaacg tcggtttatc ccccatgaaa tagcggtagt
    18901 aagcatcata agccagcacg ttcttcacat aaccgcgcgt ctcggagaac ggaatactct
    18961 cgacaaatgc cacagcgtcg atacgcccgg cgctgttgcc aagccaggtt cgcacccgtc
    19021 caggtccggc gttataagct gctgaggaga aaatacggtt attgccaaac tgctgataaa
    19081 catattgcag gtaactggtg ccaatgttga tgttcgtttc cggatccagc aattgcccag
    19141 ggctgctata accaggaata gagaacatct tcaccgtatg ggtcgctgta ccaggcataa
    19201 tctgcatcag gccgctggcc cctaccggtg atttcacttt cggattccag gcgctctcct
    19261 gacgggcaat cgccatcgca tagctttgcg ggatctcttt cccactggtg taacgtttga
    19321 aaagatcgtt gtaagccagc gggaatcgct cttccagatg atcccaaagc ttcccggcga
    19381 tcgttgcctg aacgctaaga tcccaccatt ggttattgaa agcataccga gccagttgag
    19441 cctgctctgt ttttgacttg ctcttcacca gattggccca ctcgctacgc gcggtattat
    19501 cgagattcca gtacatcaac tcgcgcacgc gcgccatctc cggcccctga gtcagggtgc
    19561 tgtcaacatt ctgcggcgct ttatcaatct tcagctcata ctcttcaccg atgcgttgtg
    19621 ctgcaaccat cgggtagaaa ccacgctgtt gtatgagttg atgcaaaatc tctttcgctt
    19681 cagcttcacg tccgcgttcc agcaataaat ccgcctgcca gtaacgccat tcatctttct
    19741 cttttgcttc catcggcaga cgcgccagcc aggtattcag gccgcgacga tcgccggtgc
    19801 caagcgccat tcgcacacgg cgttcaatca acgacgtgga ttgcgagcgc ataatggcgt
    19861 catcgcgcca tttcgcctgc tcatcggtga catcattgcc catcaaacgc caggcgacga
    19921 tatcgcgcag ctcctgaatc tgatcttcat taagctgctg cgcctgggca agcgacggga
    19981 tcatcagccg tgcattttcg gcatcctgcc gcgccacact ggcaaacgcc accgccgcca
    20041 tttgacgggt aaaatcggtc gcgccggttg tacgcgcgaa ggtcagtacc gtattagggt
    20101 tgttcgccag tgaaatgatt gccgaggcga tagtctggta atcggcaggc atctgccctg
    20161 ccagcactgt taccaggcct gtgttacccg ctttcatcgc cagacggata cgctctaaat
    20221 acgccagcgg atcttgttta cctgacgcac gccagacgct aaataacttg tcacaggcgt
    20281 taggctggct cttgccggtt agccacagct ctttcgcccc ttgccaggct tcttcactct
    20341 gcccggtgtt ccatttcgca tagtagtaat tacattgcgc ttcggtagtt ccgggctttt
    20401 ccgggctaaa ggctaacaag ccacgccagt cttcacgccg cgccagttca ttgacgaaac
    20461 gagattgcag cgtgcgagcg ggaggaagcg tggggttagc gcgaacaaag ttagtgaccg
    20521 tcaccgccgg ttgattcatc aggtcatcgg tgatctggcg gtattccagg tagggataaa
    20581 gcggataatc cttcagtcca ggcatcattt gttcgaccac atccatctgt cgattatccc
    20641 aggcctgctt gatttgcgcg taacggctac gctgctcatc cagtgagtcg gctcgcgcca
    20701 cgctgctgac cgtcagcaga cagacaccgg cagccaacag ccgccaggta acttgtttgg
    20761 ctttttccac aagcgcatcc tctaagtgta aatacatcaa tgcagcgtca tgccgcgtaa
    20821 tagatttatg ctaaccagtc attgccgttt acgccacgtt acggacactt ttttactttt
    20881 actgcgaagg tgatcgacca actgggatta ggcggggaaa caggctacac ttcgcctttg
    20941 aaaactcatc atcacgataa acatagaggc gaagtccaac gtggctcaat tcgtttatac
    21001 catgcatcgt gtcggcaaag ttgttccgcc gaaacgtcat attttgaaaa acatctctct
    21061 gagtttcttc cctggggcaa aaattggtgt cctgggtctg aacggcgcgg gtaagtccac
    21121 cctgctgcgc attatggcgg gcattgataa agacatcgaa ggtgaagcgc gtccgcagcc
    21181 agacatcaag attggttatc tgccgcagga accgcagctg aacccggaac acaccgtgcg
    21241 tgagtccatt gaagaagcgg tttcagaagt ggttaacgcc ctgaaacgcc tggatgaagt
    21301 gtatgcgctg tacgccgatc cggatgccga ttttgacaag ctggccgctg aacaaggccg
    21361 tctggaagag atcattcagg ctcacgacgg tcataacctg aacgtacagc tggagcgtgc
    21421 ggcggatgcg ctgcgtctgc cagattggga cgcgaaaatc gctaacctct ccggtggtga
    21481 gcgtcgtcgc gtggcgttgt gccgcctgct gctggaaaaa ccagacatgc tgctgctcga
    21541 cgaaccgacc aaccacctgg atgccgaatc cgtggcgtgg ctggaacgct tcctgcacga
    21601 cttcgaaggc accgtggtgg cgattactca cgaccgttac ttcctcgata acgttgcagg
    21661 ctggatcctc gaactggacc gcggtgaagg tattccgtgg gaaggtaact actcctcctg
    21721 gctggagcag aaagatcagc gtctggcgca ggaagcttca caagaagcgg cgcgtcgtaa
    21781 gtcgatcgag aaagagctgg agtgggtacg tcagggaact aaaggccgcc agtcgaaagg
    21841 taaagcacgt ctggcacgct ttgaagagct gaacagcacc gaataccaga aacgtaacga
    21901 aaccaacgaa ctgtttattc cacctggacc gcgtctgggc gataaagtgc tggaagtcag
    21961 caacctgcgt aaatcctacg gcgatcgcct gctgattgat gacctgagct tctcgatccc
    22021 gaaaggggcg atcgtcggga tcatcggtcc gaacggcgcg ggtaaatcga ccctgttccg
    22081 tatgatctcc ggtcaggaac agccggacag cggcaccatc actttgggtg aaacggtgaa
    22141 actggcatcg gttgatcagt tccgtgactc aatggataac agcaaaaccg tttgggaaga
    22201 agtttccggc gggctggata ttatgaagat cggcaacacc gagatgccaa gccgcgccta
    22261 cgttggccgc tttaacttta aaggggttga tcagggtaaa cgtgttggcg aactttccgg
    22321 tggtgagcgc ggtcgtctgc atctggcgaa gctgctgcag gttggcggca acatgctgct
    22381 gctcgacgaa ccaaccaacg acctggatat cgaaaccctg cgcgcgctgg aaaacgccct
    22441 gctggagttc ccgggctgtg cgatggttat ctcgcacgac cgttggttcc tcgaccgtat
    22501 cgccacgcac atcctggact accaggatga aggtaaagtt gagttcttcg aaggtaactt
    22561 tactgagtac gaagagtaca aaaaacgcac gctgggcgca gacgcgctgg agccgaagcg
    22621 tatcaagtac aagcgtattg cgaagtaatg cgtaaaatgc cggatgcggc gcgaacgcct
    22681 tatccggcct acaaaaccag caatttcaat acgttgtaaa actgtaggcc tgataagacg
    22741 cggtaagcgt cgcatcaggc attgagcacc gaatgccgga tgcggcgtga acaccttttc
    22801 cggtctacaa cagccttagc atcctcaatt tcgtggcggg tcacccgtgc aggttttttc
    22861 aaaccttgtt taatctcgac agcctcttcc agagaggttt tcaactcatc aaaaaaactc
    22921 attttatacc tcgcctttga gcagtttcgt cacggttatc cttgctcccc catcatctcc
    22981 cgcaccagct ccacgcagcg caggaaacgg ctgtcgtaat cgtcctcttc aacccgcacg
    23041 aattcgatat tgttctcttc cagcatctcc accagcaagt tctggaactc tttgcgatcc
    23101 accgaactgc cgaggctgcg taaaccatcc gccacccacg gcgtgttgtt ctccagcagg
    23161 atcaccagat cgaaacggta ttcatcaatc agcgcctgca cgaacggatg ctcacgccct
    23221 tcgtactttt tgcagaacgc ctgggtagtg acaaaatcgg tgtcaataaa cgccacttta
    23281 ttggcgtatt tcactgcaaa atcaatgtat tgtgcgtggc ccaacgcgat tttatcgtag
    23341 tcggaatact gcaatgcgat ctcatcaccg ccgaggtgtg aaaagacata atcgcggcca
    23401 tattcccacg cactggtggt gttgaagata ttggcaagtt tgtttaccag ggtggattta
    23461 ccgctcgact cgccgccaag gatcgccacg gtacgcacaa aaaacggctt cacttcggta
    23521 ggaatatatt cccagtagcg gaacgggttt tcgcggatct gcgcaccgct gatactcata
    23581 aaggtacgtt tcggatcgac cagcaccgtc tcgatcccca gatgttccat atactgtggc
    23641 gcatcggctt cttccgaggt gtagatcagg tccggctgga tccccttttc agccataaac
    23701 tttttgatgc cgttgctcca cacatcccag ccatgcggat acggctccat gccctcttcg
    23761 ttgaaagcat gaatacggat atttttctga tatttaaagg tttgcaatag ccagcgcaga
    23821 cgatccggca ccgtgggctg ctgcgacatg gcgctgtctt caaacaacgc gcggtcgcgg
    23881 gtatcgtcaa aacccataat gatatgcagc tcgtcaacct ggctacaggc gcgctggata
    23941 aggtagatat gtccggtatg cagcgggtag aacttaccga atacgacacc aatggttttc
    24001 ttctgccggg gaaattcaag ccccaaaaaa cggtgcaatg cctccagctt ttgcgcgctg
    24061 gggcttttaa ttttggcatt cagtaactgg cttaaatacc ctttggtcat accgctggca
    24121 tcagctacct gctgtagcgt gcagccctgt tgcttgatgg cagttttcag gtaatcaaat
    24181 gacgacatat ctccctccgt atctctcatt ataagtcgtc gaacacgcta agcgcgtcgg
    24241 agagtttttt aacgccaaaa atctgcatcc cttccggcgc ttttttcggt acgttagccg
    24301 ccggaacaat cgcccggcga aaaccgtgtt tcgccgcttc agaaatccgc tcctggccac
    24361 tgggcaccgg gcggatctcc cctgccagcc cgacttcacc aaacaccacc agatcctgtg
    24421 gcagcggtct gtcacgcagg ctggaaacca tcgccagcag taacgctaag tcgccgctgg
    24481 tttcggttac cttcacgccg ccgaccacgt tcacaaacac atcctgatcg gccatttgca
    24541 gaccaccgtg acggtgcaag acagccagca ggattgccag acggttttgt tccagcccca
    24601 ctgccacgcg gcgcggattc gccattatcg agtgatcgac cagcgcctga atctccacca
    24661 gcaacggacg cgttccttcc cacaccacca tcacggagct gccggaggtc acttcatctc
    24721 cgcgacttaa gaaaattgcc gaagggttgc tgacttcacg cagcccctgt tcggtcatcg
    24781 cgaagacgcc cagctcattc accgcgccga agcggttttt atggctgcgt aaggtgcgaa
    24841 aacgggagtc ggcatcgcca tccagaagca ccgaacagtc gatacagtgt tccagcactt
    24901 tcgggccagc cagcgaacca tctttggtta cgtgtccaac catgacaatc gccacaccgc
    24961 gcgttttggc gaaacgcgtc agataagccg ccgtttcacg cacctgcgcc acgctaccag
    25021 gcgatgattg tacatccgcc atatgcatca cctggatcga gtcaattacc atcagcttcg
    25081 gttgctcttc ttcggcaatc aggcagatct gctcgatgct ggtttccgac aacatattga
    25141 gattgtcagt cggcaggcca aggcgatgag cgcgcattgc cacctgttgc agcgactctt
    25201 cgccggtgac atacagcgtt ttcatctgct gggccagttt gcacagcgtt tgcagcaaca
    25261 gcgtggattt ccccgcacca gggttaccgc caatcagaat ggcacttcct ggcaccacgc
    25321 cgcctcctag tacgcggtcg aactctttaa atccggtgga aaaacgcggc agctcttcaa
    25381 ggctgatatc ggagagtttc tggacttttg ccaccccggc gctaccggca tagccgctga
    25441 gacgctcgtt acgcgccacc gttggcgacg cagcaagacg cacctcggtg atggtgttcc
    25501 aggcatgaca ggcactgcac tgcccctgcc agcgcggata atcggccccg cattcattac
    25561 aaacaaaggc gcgttttgga gcttttgcca cggtgtacct cgttaatgct gtgccgcccg
    25621 caggatgacg ggcaggcaat tacttctgat tcaggctgcc agagaggatg cagaataccc
    25681 ccatcaggtc agcgtgacgg atggtgactt ccgccttttc attcactttt ggcttggcat
    25741 ggtaggcaat ccccagccct gctgctttga tcatcggcag gtcattagct ccatcgccaa
    25801 tcgccacggt ctgcgccagc gggatttcat actcctgcgc gaggcgagtc agagttttcg
    25861 ctttgtactg cgcgtctacg atgtcgccga tcacattgcc ggtaaattta ccgtccatga
    25921 tctccagttc attggctacc acggctgtca ggcgcagctt gtcgcgcagg tattcagcaa
    25981 agaaagtaaa gccgccggag gcaatcgcca ctttccagcc cagcgtttcc agcttgagca
    26041 ccagctgcgt taagcctggc atcagcggca gattttcacg cacctgttgc agaatattgg
    26101 cgtcagcgcc tttcagcgtc gccacgcggc tgcgcaggct ggcggtaaaa tcgagttcgc
    26161 cgcgcatcgc ccgttcggtt acctccgcca ccatctcgcc cgttccggcc agcttggcaa
    26221 tttcatcaat acattcaatc tggatggcgg tggaatccat gtccatcacc agcaaacccg
    26281 gcgtgcgcag gtgcgggatt ttccccagcg gggcgacatc cagctgcgct tcgtgggcca
    26341 ggcgtgtagc ccgtgcggtg agtgaacctg ccagacgaat cacctgataa tcttccacgc
    26401 accaggcggc aacaatcacc atcgccgcac ccagtttgct ctggtattgg gtcagacgct
    26461 gtttatccag cccacgacca tacagcagcc agccgctacg acctgcgtgg taatccagtg
    26521 gcatcacttc atcaccactt aatgaaagag gcagacccgg ccataaagag acatcttcag
    26581 gcaggtcgca ccaggtaatg ttaggcatta aggctcctgt aaaatcgttc gaagcaggga
    26641 aaataacgca tgaggctacc ttgtatccat tgcttctggc aacattaagt ctcaaatttt
    26701 caaagggtgg aagatggctc gcacaaaact gaaattccgg ctacatcggg cagtgattgt
    26761 cctgttctgt cttgccttgt tagtggcact gatgcagggg gcgtcatggt ttagtcaaaa
    26821 ccaccagcga cagcgtaatc cacagctgga agaactggcc cgcaccctgg cgcgtcaggt
    26881 gacgctgaac gttgcaccgc tgatgcgtac cgactcaccg gatgaaaaac gcattcaggc
    26941 gatcctcgat cagttaacag atgaaagccg tatcctcgac gcgggtgtgt atgacgaaca
    27001 aggcgatctt atcgcacgtt ctggcgaaag cgtcgaagtg cgcgatcggc tggcgctcga
    27061 cggtaaaaaa gcaggcggct attttaacca gcagattgtc gagccaattg cgggtaaaaa
    27121 cggaccgctc ggctatctgc gcctgacact cgacacccat acgctcgcca ccgaagccca
    27181 acaggtggat aacaccacta acattttacg cctgatgttg ctgctctcac tggcaatcgg
    27241 cgtagtgctg acccgcacgc tgctacaggg taaacgcacc cgctggcagc aatcgccctt
    27301 cctgttaacc gccagcaaac cggtgccgga agaggaagaa agcgagaaaa aagagtgacc
    27361 cattactaca agaaaggaaa tcgttatgtc cacattacgc ctgctcatct ctgactctta
    27421 cgacccgtgg tttaacctgg cggtggaaga gtgtattttt cgccaaatgc ccgccacgca
    27481 gcgcgttctg tttctctggc gcaatgccga cacggtagta attggtcgcg cgcagaaccc
    27541 gtggaaagag tgtaataccc ggcggatgga agaagataac gtccgcctgg cgcggcgcag
    27601 tggcggtggc ggcgcggtgt tccacgatct cggcaatacc tgctttacct ttatggctgg
    27661 caagccggag tacgataaaa ctctctccac gtcgattgtg ctcaatgcgc tgaacgcgct
    27721 cggcgtcagc gccgaagcgt ccggacgtaa cgatctggtg gtgaaaaccg tcgaaggcga
    27781 ccgcaaagtc tcaggctcgg cctatcgcga aaccaaagat cgcggcttcc accacggcac
    27841 cttgctactc aatgccgacc tcagccgcct ggcaaactat ctcaatccgg ataaaaagaa
    27901 actggcggcg aaaggcatta cttcagtgcg ttcccgcgtg accaacctca ccgagctgct
    27961 gccggggctc accatgagca ggtttgcgag gccataaccg aggccttttt cgcccattat
    28021 ggcgagcacg tggaagcgga aatcatctcc ccggacaaaa cgccagactt gccaaacttc
    28081 gccgaaacct ttgcccgtca gagtagctga gagtggaact tcggtcaggc tccggcattc
    28141 tcgcatctgc tggatgaacg ctttagctgg ggcggcgtgg aactgcattt cgacgttgaa
    28201 aaaggccata tcacccgcgc ccaggtattt accgacagcc tcaacctcgc gccgctggaa
    28261 gccctcgccg ggcgactgca aggctgcctg taccgcgcgg atatgctgca acaagagtgc
    28321 gaagcgctgt tggttgactt cccggaccag gaaaaagagc tacgggagtt gtcgacgtgg
    28381 atagcggggg cggtaaggta actcgctgat taaaatatcc aaggaaggaa atatgatcga
    28441 tctggaaaac caggaacggg agattatcaa tttaatgttc agccagcgta tttcctggct
    28501 agccgccgtg cgtatcaggc ataaattatc gcttgctgaa gtaagtaaaa tgttaggtat
    28561 ctcgattaat tctctaaaac agatcgaaaa gacggaacgg cttagctcaa acatcaaaag
    28621 caaaatggca gaaatttatg gttgtccacc agagttgctg atatgccctt cctggatgac
    28681 ggctgaacat aaatgaatag attcgtgaat agatacttga atgtttttta ttttctatcc
    28741 atatgaaaaa taatagtaaa tctataaatc agttgctatt gatgaataga tactaaaaag
    28801 ccggagcagt cccccactcc ggcttaacac gacaatcgcc tttcagcgaa acacaattac
    28861 tctttatcgc ccagcagaac ggattccagt gcgattttga tcatgtcgtt gaaggtggtc
    28921 tgacgctcag cggcagtggt ctgctcgtga gtgcggatgt ggtcagacac ggtgcagatg
    28981 gtcagggctt tcgcgccaaa ctctgcagcc acgccgtaga taccagccgc ttccatttcc
    29041 acgccgagga tgccgtattt ttccatcacg tcgaacattt cgccgtccgg agagtagaac
    29101 aggtcagcgg agaacaggtt acccacgcga gcatcaacgc ccagtgcttt agctgcatct
    29161 actgcgttac gcaccatgtc aaagtcagcg atagcggcaa agtcatggtc tttaaaacgg
    29221 atgcggttaa ctttggaatc ggtgcaggcc cccataccga taacgacgtc gcgcagtttt
    29281 acgtgcggca gaactgcgcc acaggaacca acgcggataa ttttcttcac gccgaaatcg
    29341 gtgatcagtt ctttggtgta gatggagcag gacgggatac ccataccgtg acccattacg
    29401 gaaattttgc ggcctttgta agtaccagtg aagcccagca taccgcgaac gttgttcact
    29461 tcacgggcat cttcaaggaa agtttcagca atatacttcg cacgcagcgg gtcgcctggc
    29521 atcaaaacta cgtcagcgaa atcgcccatt tctgcattaa tgtgtggggt agccattgtt
    29581 ttatcctttt tgtgacataa caaaggcgga gtcaacgctc cgcccaaatc catcagaaca
    29641 tggctttgcc atattccata tcagaagtac caaaatattt tgccagagtc tggccgatat
    29701 ccgcgaaggt ttcacggtga cccagtgagc ccggttttac tttcgggcca tataccagta
    29761 ccggaatgtg ttcacgcgtg tggtcagtac cggtccaggt cggatcgcaa ccgtggtcag
    29821 cggtgaggat caggatgtcg tcatcgcgca gcagagacat cagctccggc agacggcggt
    29881 cgaacagttc cagacccgca gcataaccgg cgacgtcgcg gcggtggccc caggaagagt
    29941 cgaagtcaac gaagttggtg aagacgatgg tgttatcacc cgcttctttc atctctttga
    30001 tggtggcgtc aaacagcgcg tccaagccag tcgctttcac ttttttggtg ataccgcagt
    30061 tggcgtagat gtctgcaatt ttaccgacag aaaccacctg gccgtgtttt tcatcaacca
    30121 gtttctgcag cacggtcggt gctggcggct caacagccaa gtcgtgacgg ttaccggtac
    30181 gctggaagtt accagctttg tcaccgataa acggacgagc gataacacga ccgatattgt
    30241 agccgccgtt ggtcagctct tcacgggcga tttcgcacag ttcgtagagt ttatccagac
    30301 cgaaagtttc ttcatggcag gcaatctgga acacggagtc agcggaggta tagaaaatcg
    30361 gcttgccggt tttcatgtgc tcttcgccca gttgatccag aatgaccgta ccggaagagt
    30421 ggcagttacc gaggtaaccc ggcagattag cgcgttcgac cagtttatcc agcagctctt
    30481 gcgggaagct gttttcgtga tcggagaaat atccccactc aaacagaacc gggacaccgg
    30541 cgatttccca gtgaccagac ggggtatctt taccggatga catttcgtgc gcccatgcgt
    30601 acgcgccgat aacttcagcg ttgccgtcca ttcccgccgg aatgaaaccg gtagaacctt
    30661 cgtgtgcttt cgccagcccc agacgggtca gatttggcag attgagcggc cctttacgac
    30721 cattatcagc ttcgcctttg gcacaagctt ctgcgatatg acccagggtg tcagccccga
    30781 cgtcaccaaa gcgttctgca tcttctgtag cgccgatgcc gaatgagtcc agcaccataa
    30841 taaatgcacg tttcatatgt tctccgtact cagtgcttca aatttaaatg atcagatcag
    30901 tataccgtta ttcgctgata cggcgataga cagttggtgt gctttccggt gcgttatcgg
    30961 caagtttaat tgccgctttc accgctttcg ccgcttcctg ccagctgttt tcgtctttcg
    31021 cgaggataac cgccagcgga cgctgaccgt ctacctggtc gcccagacgc gccatatcag
    31081 taaagccgac gctgtaatcg atggtgtcag atgcctgacg acgaccgccg cccattgcaa
    31141 ccactgccat ccccagcgcg cgggtatcca tttcactgac aaaaccttcg gtatcagcat
    31201 agactgcttt cgtcagcatc gctgtcggca gatacttcgc gtagttctca acgaagtcgg
    31261 tcgggccttt ttgtgccgct accatacgac caaagacttc tgccgcttta ccgttgtcca
    31321 gcaccgcctg caatttcgcg cgcgcttcgg cgtcatcttt cgccagtttg ccggagatca
    31381 gcatctccac gcacagcgcc atcgtgacat caaacagacg cgggttacga tactcacccg
    31441 tcaggaactg caccgcttca cgaacttcaa ccgcgttacc tgcactggag gccagtacct
    31501 gattcatgtc ggtgagcagc gcggtggtgc gcacgccagc gccgttagcc acgccaacaa
    31561 tcgcttcggc aagggcttca gagagttcgt aggtcggcat aaacgcgccg ctacccactt
    31621 tcacgtccat caccagcgcg tccagacctt ccgccagttt cttcgccagg atcgaggcag
    31681 tgatcagcgg gatggagtcc acggttgcgg taatatcacg ggtcgcgtag aaacgtttat
    31741 cagccggagc cagtgaactg gtctgaccga taatcgccac gccgacgtct ttaataattt
    31801 cgcggaaacg gttgtcatcc gggaaaatgt cgaagccagg gatggattcc agtttgtcga
    31861 gcgtaccgcc agtatgaccg aggccgcgac cggagatcat cggaatatag ccgccgcagg
    31921 ctgcgaccat cggccccaac atcagcgaag tgacatcgcc gacgccgccg gtcgagtgtt
    31981 tatcaacaat cgggccattc agatgcaggc ttttccagtc gagaacggtt cctgaatctc
    32041 gcatcgccat ggtcagcgag acacgctcag gcattgtcat atcgtggaag aaaatggtca
    32101 tcgcgagggc ggcaatctgc ccttcggaaa tagtgttgtc gcgaataccg ttgataaaga
    32161 aacgaatttc ttcatcgctc agcgcatgac catcacgttt tttacgaata atttcttgtg
    32221 cgagaaacaa ggtaacctcc cgaggaaaag agtatgaggc ggcttttgcc ggatgcgtct
    32281 acgccttatc cggcctacgg gtagatacgt ctcgtaggcc tgataagcgc agcgcatcag
    32341 gcgtaaagca tcttacttag tagctgctgg cgctcttacc gtcgccgtgg cccagcgctt
    32401 tcagcaggct tgccagcagg ctggaagcac caaagcggta gtgacgcgca tcagcccagt
    32461 cagcaccgaa cagttcatct gcgatggcga gatatttctg cgcatcttcc gcagtacgca
    32521 cgccgcccgc cggtttgaaa ccaacggttt tttctacgcc catatcacgg atcacttcca
    32581 tcatgatgcg agcgctttcc ggcgtcgcgt tcacagccac tttaccggta gaggttttga
    32641 tgaagtccgc acccgctttg atggagattt cagacgcttt acggatcagc gcttcgtctt
    32701 tcagttcgcc ggtttcgatg atcactttca gcagtacatt cgctgccgcg caagcctctt
    32761 tacaggcttt caccaggtca aaaccaacct gctcgtcacc cgccatcagc gcgcggtacg
    32821 ggaacaccac gtcaacttca tcagcaccgt aggcgattgc cgcacgggtt tctgccagcg
    32881 cgatttcgat gtcgtcgtta ccgtgtggga agttggttac cgtagcgata cggatttccg
    32941 gggtgccctg ctctttcaga gttttgcgag caatcgggat aaaacgagga tagatacaga
    33001 tagcggcggt attgccgacc ggagttttgg cctgatgaca cagagcaatt actttctcgt
    33061 cggtgtcgtc gtcattcagg gtggtcaggt ccatcaattt cagtgcacgc aggctgcttg
    33121 ctttcagatc agtcatttca ttctcctggc ttgtcgccaa taaaattcac cttgcgagtt
    33181 tgttagtatt ctaacatcta ctccgcaaca cacttcgata cacatcacaa ttaaggaaat
    33241 ctacttacaa gtttgcatca ctgtaatgcg atctggttca aataattcac tttcaaatga
    33301 atgcgtcagt ggtggcaaac gcatcaggat caaagtgaac atcacgaaac ttcttacaat
    33361 ggcgcatcac cttcggcata aaaggaaaag aagatgccca cttctcatga aaatgcactg
    33421 caacaacgtt gccagcaaat tgtcaccagc ccagtgctta gcccggagca gaagcgccat
    33481 tttctggcac tggaagcaga aaacaatctg ccttacccac agcttcctgc cgaagcccgc
    33541 cgcgcgctgg atgaaggtgt aatctgcgat atgtttgaag gtcatgcgcc gtacaaaccg
    33601 cgctatgtct tacccgatta cgctcgtttt ctggcgaacg gttccgaatg gctggagctg
    33661 gaaggcgcga aagatcttga tgacgcactc tctctgctga ccattcttta ccaccacgta
    33721 ccgtcggtca catcgatgcc ggtctacctg gggcaactgg atgcgttgtt gcaaccgtat
    33781 gttagaattc taacacaaaa cgagatcgat gttcgaataa aacgtttctg gcgttacctc
    33841 gacagaaccc tgccagatgc ctttatgcac gccaatatcg gcccgtctga ttcgcccatt
    33901 acccgcgcga tcttacgtgc agacgcgcag gtttcaccga acctgacctt tatctacgat
    33961 cctgaaatca cccctgatga cctgctgctg gaagtggcga agaacatctg tgaatgtagc
    34021 aaaccgcaca tcgccaacgg tccggtgcat gataaaattt tcacaaaagg gggctacggg
    34081 attgtgagct gttacaactc actgccgctg gcgggtggtg gcagcacgct ggtacgcctt
    34141 aacctgaaag ccattgccga gcgcagcgaa tcgctggatg acttctttac gcgcactcta
    34201 ccgcactact gccagcagca gatcgccatc atcgatgcgc ggtgtgaatt cctctatcaa
    34261 caatcacact tctttgagaa tagcttcctg gtgaaagaag ggctgattaa ccctgaacgt
    34321 tttgtgccaa tgtttggcat gtatgggctg gcggaagcgg ttaacttgct gtgtgagaaa
    34381 gaagggattg ccgcgcgcta cggtaaagaa gccgccgcaa atgaagtggg ttatcgcatc
    34441 agcgcgcaac tggcggagtt tgtcgccaat acccccgtga aatatggctg gcaaaaacgc
    34501 gccatgttac acgcacagtc ggggatcagt tccgatatcg gcaccacgcc gggcgcgcgt
    34561 ttgccgtatg gcgatgagcc agatccgatc acccatctgc aaactgtcgc cccgcatcat
    34621 gcttattatt attccggcat cagcgacatt ctgacgctcg acgaaaccat caaacgtaac
    34681 ccgcaggcac tggtacagct ttgcctcggt gcctttaaag ccggaatgcg tgaatttacc
    34741 gccaatgtca gcggtaacga tctggttcgc gttaccggtt atatggtgcg tttgtcggat
    34801 ttagaaaaat atcgcgccga aggttcacgc accaacacca cctggctggg cgaagaagcc
    34861 gcacgcaaca ctcgtattct ggaacgccag ccgcgcgtga taagccatga acagcagatg
    34921 cgctttagtc agtaagatta tccccttctc ctgcgttgac gggccaggca gtcgtctggc
    34981 tctgtttttg cagggctgca atctgcgctg caaaaactgt cacaatccgt ggacgatggg
    35041 acgttgcaat gactgtgggg agtgcgtgcc acagtgtccg catcaggcgt tgcagattgt
    35101 tgacggcaaa gtggtgtgga acgctgtggt ctgcgagcaa tgtgatacct gcctgaagat
    35161 gtgtccgcaa catgccacac caatggcgca atccatgagc gtggacgaag tgcttagcca
    35221 tgtgcgcaaa gcggtgctgt ttattgaagg gataacggta agcggcggcg aagccacgac
    35281 ccaactgccg tttgtagtgg cgctgtttac tgctatcaaa aacgatccgc aactgcgcca
    35341 tctcacctgt ctggtggaca gtaacggtat gttgagcgaa accggctggg aaaaattact
    35401 cccggtgtgt gacggcgcaa tgctcgatct caaagcatgg ggtagcgaat gtcatcaaca
    35461 attaaccgga cgcgataatc aacagattaa gcgcagcatc tatttgctgg cagagcgcgg
    35521 caagctggcg gaactgcgtt tgctgatgat cccaggccag gtggattatt tgcaacacat
    35581 cgaagaactg gcggcgttta tcaagggact tggtgatgtt ccggttcgcc tgaacgcgtt
    35641 tcatgcccac ggcgtgtatg gcgaggcgca aagctgggcg agcgccacgc cggaagacgt
    35701 tgagccgttg gctgatgcat taaaggtgcg cggggtgagc cggttgatat ttccggcgct
    35761 ctatttgtga catgccggat acggcctacg gttcggcaca gacttgtagg cctgataaga
    35821 cgcgtcaagc gtcgcatcag gcattgtgcg ccaactgccg gatgcggcgt gaacgcctta
    35881 tccggcctac ggttcggcac aaacctgtag gcatgataag acgcgttaag cgtcgcatca
    35941 ggcattatgc gccgactgcc ggatgctgcc tacgaagggc aaactaccca cgaacgttaa
    36001 acaccgcata cgtgttatta agcaacactt cggcaatctc atccgcgggt tccgggcgta
    36061 actcgcaaag cacggcaaac acacgggcag cctgttccgg gcggttaggc tgcccctgaa
    36121 aaccgttgag cggcatatcc ggcgcatcgg tttccagcaa caacgatgcc agcggtaatt
    36181 ttgcgatgac atcgcgggtt ttactggcgc gtggataggt gatagtaccg cctacgccga
    36241 ttttgtagcc cagctgtaca aaccgttcag cctgttgctg gctgccagaa aatccgtgga
    36301 caacaccagc acgcggcaaa tcgtggtgtt taagatgcat cgccagtttg tcgtgcgtgc
    36361 gccgtgaatg caggatcacc ggcagatcgt agcgtttcgc cagtttcagt tgttcgtcga
    36421 gtaaccactg ctgcctctca aattgcggat cgtcgccgaa gagatccaga ccgatctccc
    36481 ccaccgccac caccttcgcc ggacgccttt ccagcgcctg ctgtagctgc tcaagagaca
    36541 catcgctatg tttttccaac ataccaggat gcaagcccaa tgcggcatac agcggttgat
    36601 aattttccgc taacgcctgc acgcgggcaa aattttccgc ctctgtcgcc ggaacaatga
    36661 tcttgcctac gcccgcttgt gccgcgcgtt gcaggctggc ctcttcatcg ccaataaaag
    36721 gcgggaaatc aaaatggcag tgagtgtcga taaaacggca aatcaagcca gatcctcatt
    36781 gttaaatgtc gggtcgttcg cctgcggagc gtcgctcact tcggcaacca gcgtatcgtt
    36841 ggcgactggc gcaggcggaa tgacaatcga ttccggcgtc accactggca ccagatggcg
    36901 ggtaagcggc gctttttcag ttaatagctt gccaaccgtg gcgaggaaat aacgaccgca
    36961 taaacgccca agtttatagt cttcacgcag cgccggaatc cgactgccaa gcgcgatact
    37021 atgtaatggc ttcggcggat aaatttcgga tatccgcagc ttgcccggtg gtttctcaat
    37081 aaattgctga atgtcacgat agctggtttc atgatgctgc accagattga ccagcggctg
    37141 caggctactg tcacccagcc agcgttccat gcgtttgaac cactgcggcg tgtagtacat
    37201 ttgtgacggc acagtgcgga tgacgaccaa cgttttagcg ccctgccttg ccgcctcttt
    37261 aaccggaatc gcatcactga tcccgccatc caggtagtta atgccttcca gcgacactcc
    37321 gctacgataa aagccaggta tcgcactgga ggcgcgaatc acatccagcc agttttgttt
    37381 ggttggtaaa aagtaattcg gctcgtagtc atcctgacga caggcgcaca tataaaacga
    37441 tttgccgctg tcaaacaacc gcgcggcggt atccatttgc aacggcatct ggcttgcggt
    37501 ggcctccacc agccagtcga gatcgataag atttcctcca cggacaaagc gcaatggatc
    37561 gaaaaattcg cgttttgtgg tatagcgcat gatgactttg cgcgcgtaac cgggctgatt
    37621 gcagataaac gccgagaggt tctgcgcccc ggcagatgtg ccgagataaa gatcgaaagg
    37681 attaaactgc gcgcgcataa actcatccag cacgccagcc gtgaaaattc cacgctgtcc
    37741 gccgccttcg cacaccagag ctattcgtcc aggctggaac ggccttagcg acaacggcgc
    37801 aatattacca agcgttacag gtattcgctg ccccacttct gccttcctgt ttttgttgtg
    37861 aaagaaagac aaagtaacgc aatttattat tcgctaaaac cgcaaaagcc agtccgctgg
    37921 actggctttg gataaaattt tcttaatggt ctgaaatcta gggtcgtttt cggcccatga
    37981 acaaactcac taggaacaga ataatcccga cgacaaagac aattttagct gcgcctgcag
    38041 cggtaccggc cagaccacca aacccaagtg cggcggcgat taacgcgata accagaaata
    38101 tgatgcccca acgaaacata agcgtctcct ttaccatagt taatgtcacc gcttgatatg
    38161 agcgtgaaaa tcactcaatg cgatattttt agtgtggtgc acatcgcgcc tcccgacaaa
    38221 gttcgggagg acgaattact tagttttcag atcattttta acgcttttca caccatctac
    38281 cgctttggcg atactttcag cacggtcact ttgtgcctga gaatctacgg taccggagag
    38341 ctgaaccacg ccatcggtgg tttcaacttt cactttacgt gaagggacga tatcgtccgc
    38401 cagcagtttg gctttgattt cactggtggt ggcggtgtca cccgcgtagc ccttcaccga
    38461 gccttcttta gcgtcgcgaa cgtgcagttt gtcgctgaca gaggtcacgc cttcaacgcc
    38521 tttcgccact ttcactgcct cttcggcctg ggcctgactt tcaacgaaac cgctcagggt
    38581 cacgactttt tgatcggttt tgacagagat atcggtgctc ttgatgttgt catgatccac
    38641 cagcgccgcc ttcactttcg cggtgatggc gctgtcatcc atgaaattac cgactttatt
    38701 catagagcta tcgacttttt gccctgcgct ttcattggta gtctgcgcgt tgttttccgc
    38761 gtaggcagag ccggtcgcga cggcagaggt caacattaca gccagcagag ttttcgaaat
    38821 cttcagtctt gtcatagtca tcgatttatt cctgtatgtt tgctcgtaat ttgagcctag
    38881 caacacgagg ttgcattgct gaatagggag agacttcacc ctctacagaa gtcaatggtc
    38941 gccatcacaa aagcgatggg tgatgaataa cgaccattac agcctctgaa tcagttatta
    39001 atatcggtag aatgacaatc gacggcttta gatactgata tctacgcatt gaacggtatt
    39061 taacgccatc agaaatgtca tcactttgtt aaatatagat cacaattttg aaaccgctcg
    39121 ggatatcagc gaaaacataa gcaaaagtga atgttttaag aacattccgt aagcggctaa
    39181 taaggaaggg aaattgacag ggcgcagcgg ctaccgcgcc ctggaggcaa gaattaatgc
    39241 tcgcgggtct ggtggaactg aacgtccgga taacgttcct gtgccaggcg caggttgacc
    39301 atgctggtag cgatgtaagc gaggttatcg ccgccatcaa gcgccagttg gctttcgttc
    39361 ttacgcttga actcttcgaa tttcttcgcg tctgcacatt ctacccagcg ggcagtggca
    39421 acgttgactg actcatacac tgcttcaacg ttgtattcgc tcttcaggcg cgctaccacc
    39481 acatcaaact gcagcacacc aaccgcacca acgatcagat cgttgttgga gattggacgg
    39541 aacacctgca ccgcgccctc ttcggaaagc tgtaccagcc ctttgagcag ctgtttttgc
    39601 ttcagcggat ctttcaggcg gatacgacgg aacagttctg gtgcgaagtt cggaataccg
    39661 gtgaacttca tcatctcacc ctgggtaaag gtgtcgccga tctgaatggt gccgtggttg
    39721 tgcaggccga ggatatcgcc cggatacgct tcttcaacgt gcgaacggtc acccgccata
    39781 aaggtcagcg cgtcggagat caccacatct ttcgcagtgc gcacctggcg cagtttcatg
    39841 cctttttcat atttaccgga caccacacgc ataaacgcca cgcggtcgcg gtgtttcgga
    39901 tccatgttgg cctgaatttt aaatacgaag ccggtaaatt tatcttcgct cgcttctacg
    39961 gtacgggtat cggtctgacg cggcatcggc gcaggcgccc actccaccag gccattcagc
    40021 atatgatcaa cgccgaagtt acccagcgca gtaccgaaga ataccggagt gatttcgccc
    40081 gcaaggaaca gctctttgtc gaactcgtta gacgcgcctt taaccagttc cagttcgtca
    40141 cgcagctgct gtgccagatc ttcaccaacc gcagcatcga gatccgggtt attcagccct
    40201 ttaacaatgc ggacttcctg aatggtgtgc cctttaccgc tctgatagag ataggtttca
    40261 tctttataaa ggtggtaaac gcctttaaac agcttgccgc agccaattgg ccaagtgatc
    40321 ggtgcgcagc caattttcag ctcgttctca acttcatcga gcagttccat cgggtcgcgg
    40381 atatcacggt caagtttgtt cataaaggtg aggatcggcg tgtcgcgcag acgggtaact
    40441 tccatcagct tacgggtacg atcttcaaca ccttttgcgg cgtcgataac catcaggcag
    40501 cagtccaccg ccgtcagggt acgataggta tcttccgaga agtcttcgtg ccccggggtg
    40561 tcgagcaggt taaccaggca atcgtgatac ggaaactgca tcacagacgt agtaatggag
    40621 atcccacgct gcttttccat ctccatccag tccgacttag cgtgctggtt ggaaccacgg
    40681 ccttttactg taccggcggt ctgaatggcc tgtccgaaca gcagcacctt ctcggtgatg
    40741 gtagttttac cggcgtccgg gtgagaaata atggcaaaag tgcggcgctt cgccacctct
    40801 ttcaaataag gagacaacgt cataattctt tcttcttgag taagcggcat cgcgccgcgc
    40861 ttgttgaaaa cgaaaaattg cggctatttt acccatcaac gggggggagg caatcagtgt
    40921 ttacacagga gctgctccag ttcgtgcaac gaagaaacgg tccaggtggg cgcgatgcct
    40981 tctggttgct cgcgattgtg cgcattcagc cagcaggtcg caagcccggc gttgatgcca
    41041 ccgagaatat cggactcggc agtgtcgcca accatcagca cgcgtgaacg gtcaggattg
    41101 cccgcctgtt ccagcgcata atcgaaaatt ttcttattcg gtttggcaac gccaacttct
    41161 tcggaaatca ccagcaaatc gaagtaatca cgcaggcccg tccgttccag acgcacttgc
    41221 tgcaaggcgc taaaaccgtt ggtgatgatg ccgattttag cgttgctacg aatggcgtta
    41281 agcagagaaa ccgcgcccgg caacggcgta cagatttccg ccatcgcatt aataaaggca
    41341 tcgttaagca agcctgcttc aactttcagc cgttcggccc agctctcgaa acgcccgtgc
    41401 tgaagctgta atgaagtgat cgcgccgttt tgataatcca cccacagtgg cttgttaacg
    41461 gcctgatagt cctgaaaatc ttcagcggta aaggtgacgc tgtaatcaag aaacatccgc
    41521 tgcaggccgg tgaatgagtc aaaggtaaac agcgtttcat cggcatcaaa gaaaatccag
    41581 tcccacttca ttattccacc ttgtcttaca tactgattgg caacgccatg atgatggcgt
    41641 cttcgcgacc gtccgtggtg gggtagtaat tgcggcgaat cgtcgcctcg ttaaagccta
    41701 aactttcgta cagggcaatg gcggcagcat ttgaggcacg gacttccagc catagtgtcg
    41761 ccacgccgcg tttttccagt tcgtcgatca gatgttccag cagcgcccta cccagcccct
    41821 gacgctgata gtcgggatcg accgcaatat tgaacaatgt agcttcatcc agcaccactt
    41881 gcgtaatcgc aaacgccgcc attttgccgt tttgcgttaa ctgaaagttg agataacgct
    41941 cgccctggtt gctggcaaag gttttttcac tccacggaaa ggagtgggcg cgttgttcaa
    42001 tgtggtaagc cgctggtaaa tcagtcgttt cgagggaaga aatcgtgttc atatgtgcaa
    42061 atttgttgcc ataacgcggc gcgtgccgtt gggtttgccc gtaattcggt gagcgccggt
    42121 gatgccacct gagcgccttc caatgatagc ggttcgtcag tacccaaccg ccagctgttg
    42181 cagcgactgc cttgcggcag catcgcgatt ttttctggcg tcagttgcag cacttgatcg
    42241 gggctgacgg ttaatgcgcg cagaacatcg ctcactaaag gatcagtcag ggcgggaaga
    42301 tcgtttgcca ccatcaccag acggacgtgt gccgggctcg caatggcaat ctccccctgc
    42361 aacgcgccag ggcgacgcag cgaccactgg gtaatgccca gttgctgtaa ctgccagtct
    42421 cgtcgggatg tcatagcgaa tcgctcctgt tgtcaggggc gcaaatatag caaattcgtc
    42481 gataccgcgc caacatatgg ctataatcgc cgccagtatc aattgaggag cattccatgt
    42541 ctgcatttac cccggcaagt gaagtcttgc tgcgtcacag tgatgatttc gaacaaagcc
    42601 gtattctgtt tgccggagac ttacaggatg acctgcccgc gcgtttagat accgcggcca
    42661 gccgtgctca tacccagcaa ttccaccatt ggcaggtgtt aagctgccag atgggggata
    42721 acgcccgttt tagtctggtc gccacggcga atgatgtcgc agattgcgat acgctgattt
    42781 actactggcc gaagaacaaa ccggaagccc agttccagtt gatgaattta ctttctctgc
    42841 tgccagtggg gacagatatt tttgtcgttg gcgagaaccg cagcggcgtg cgcagcgctg
    42901 agcagatgct ggcagattat gcgccgttga ataaagtcga cagcgctcgt cgctgtggcc
    42961 tctattttgg tcgtctggaa aaacagccag tatttgatgc ggataaattc tggggcgaat
    43021 acagcgtcga tggcctgacg gtcaaaacgc tgcctggcgt gtttagccgc gacggtctgg
    43081 atgtcggtag ccagttgctg ctctcgacgt taactccgca cacgaaaggt aaagtgctgg
    43141 atgtcggctg tggcgcgggt gtactttcag ttgcctttgc gcgccattcg ccgaaaattc
    43201 gtctcacctt gtgcgatgtc tctgccccgg cggtagaagc cagccgcgca acacttgcag
    43261 ccaacggtgt tgaaggtgaa gtctttgcca gcaacgtctt ttccgaggtg aaaggttgtt
    43321 ttgatatgat catctccaac ccgccgttcc acgatgggat gcaaaccagc ctggatgcgg
    43381 cgcaaacgct gattcgtggc gcggtgcgtc atcttaatag cggcggcgag ctgcgaattg
    43441 tagcgaacgc cttcctgcct tacccggacg tgctggatga gacatttggc ttccatgaag
    43501 ttatcgcgca aaccgggcgc ttcaaggtat atcgcgccat tatgacccgc caggcgaaga
    43561 aaggttgagt atctgatatc tgtcgccgca taccgtgtcg gatgcggcgt gaacgcctta
    43621 tccgacctac aaaacctgca cgttagccct tcgtaggcca gataagacgc gccagcgtcg
    43681 catctggcat ctgcccccgc atgcctacca aacctgcacg ctagtttcct gatggacatt
    43741 tttccagcaa ttacacatca ggcgataatt aactattgac gaaaagctga aaaccactag
    43801 aatgcgcctc cgtggtagca attcttttta agaattgatg gtatgcgaag gtggcggaat
    43861 tggtagacgc gctagcttca ggtgttagtg tccttacgga cgtgggggtt caagtccccc
    43921 ccctcgcacc aaaaaccacg ttgatattgc tcgcactggg cgaaggtggc ggaattggta
    43981 gacgcgctag cttcaggtgt tagtgttctt acggacgtgg gggttcaagt cccccccctc
    44041 gcaccaaacg aggcgatatc aaaaaaagta agatgactgt gcgaaggtgg cggaattggt
    44101 agacgcgcta gcttcaggtg ttagtgtcct tacggacgtg ggggttcaag tcccccccct
    44161 cgcaccaatt atctttactt cctttcttgt ttcttccctg ttttgtcatc cctgattcaa
    44221 attcattatc atcagcatga tgccagcaat aagcgccagg cacgatggta gcaaaatgta
    44281 atgccgcaac tgtttgtgat acagcatcac cggcgtcaat actaatccca tcagaatgac
    44341 aatccgccac tgatccggcg ataaatccgc atacatcagc cctgcaatca gcaatcccgg
    44401 cagcaacact ccccagccac tgcccagcgt acgttgcaac atggtttcat ctctttcatt
    44461 gataatgaaa accaatatca tatgataatt tttatcattt gcaagccaga gaaatccctt
    44521 gctatcgagt aaacctatcg ctaggattag caatcattat catttagatt actatcacga
    44581 ttatggccta tcgttccgca ccgctctatg aagatgtcat ctggcgaacg catctccagc
    44641 cgcaggatgc tgggcttgca caagccgtac gggcgatgat cgccaaacat cgtgaacatt
    44701 tgctggagtt tatccgcctg gatgaacccg ctccgcttaa cgccatgacg ctggcgcaat
    44761 ggtcatcacc gaatgcgcta agttctctac tggcggtcta ttccgatcat atctatcgca
    44821 accaaccaac gatgatccgc gagaacaaac cgctgatctc actatgggca caatggtata
    44881 tcggcctgat ggtgccacca ttaatgctgg cgctactgac gcaggaaaaa gcattagatg
    44941 tgtcgccgga acatttccat gctgagtttc acgaaaccgg acgcgtcgcc tgtttctggg
    45001 tcgatgtgtg tgaagataaa aacgcaacac cacattcgcc gcagcagcga atggaaacgt
    45061 taatcagcca ggcgctggtt ccggttgtgc aagcactaga agcgaccgga gagatcaacg
    45121 gcaaacttat ctggagtaat accggttatt tgatcaactg gtatctcact gagatgaagc
    45181 aactgctcgg cgaggctact gttgaatcgc tgcgccatgc cctctttttt gagaaaacgc
    45241 tcacgaacgg tgaagataat ccactctggc gtaccgtggt actgcgcgac ggcctgctgg
    45301 tgcgccgcac ttgctgccag cgttatcgct taccggatgt gcagcaatgt ggctattgta
    45361 cgctgaaatg aaagcactgc cggggaagta aacccggcat catgcggatt aatagggatg
    45421 caacacatta cttgcttcgc aatgccgcat acataacaga atatctgcgg cctccaacaa
    45481 ccctgcatca gaactgacgc ccagtttcga catcacatta aatttatgcg ctcggatcgt
    45541 tttgatatta cgtttaagct gctcggcaat ttgtgtcatt gagtagccac gcgacataaa
    45601 gcgcaatatt tctctctccg tcgggcttaa cgtccggctt tggttaatgt accactgatt
    45661 gttcagtcgg tcggtcgcct gacgtacacc atttaatgac aaaaagagtt cctgatgaaa
    45721 aatctccagc gttgacgctt tacttaatac accgtccagc ggtgatggcg acaatgaacc
    45781 aatcagccga gcttcaatat catcatccac aataactaaa cgccgggtac gcggaaactt
    45841 aatcgccagc tcagtcaggc aagataatcc ctctctgcgc tcacttctca tggcagaaaa
    45901 agaaaaaatg accgccgcaa acgagatccg cgacatggca tcctgaaatg actcctgact
    45961 ggtataggta tggagcttat aagggttgcc cgcaaacttt ctggataaac tctcaatacc
    46021 gatactactc atgacgcatt tttctatgag tgcgacattt ctcttcttaa ttcggctgtg
    46081 ttccattctg cggcatctcc ataaacagaa ggcaagttaa gttctcttgc accctgcgcg
    46141 ctatttatcc atgcatacat ttcagcgttg ctgtgaattg ccagtcgacg catcgcactg
    46201 tttttttggg cgctgatagt tttattactt ttcttaagca atgaagctat ctggttgatg
    46261 ccccatcctt tacctaatag ccgcagtacc ttgcgctctg aaagagtgag aatgacggag
    46321 cgatagctaa aatcatgagt ttcagtcatt gccggagagg tcagcatggc gctgatacgc
    46381 tctgcgtgcg tattgccgga acgtacggtc ttgaccagat tctcaatggg ttcaacatca
    46441 gacaataacg tcgccgtagg gcacatgagc agttctacag cctgggaata ccaggagcgt
    46501 gacaccatga aaacccagtg aatttcccga tattgtgaga ttaaagaata ataatgttca
    46561 caaacagaac gtggatcttc actttcaccg gctaaatcag caatgactaa
//
LOCUS       DAJTQY010000024        45676 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.024, whole genome shotgun sequence.
ACCESSION   DAJTQY010000024 DAJTQY010000000
VERSION     DAJTQY010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 45676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45676
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.024"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            114..2267
                     /locus_tag="OO349_001375"
     CDS             114..2267
                     /locus_tag="OO349_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HCR5866510.1"
                     /translation="MYKSLFTIIILLYSASSYAKSESPPASTITFIAQIDHVELNKTA
                     LSHDMKLVLADRFYFKTSNPCETNSISARPESMDITDKAYADKLKKLETPFLDDRYFF
                     MTINQCDGNGTPMLTDIKVCTTELCGAEYTKKMSTVWLDETLKPTAKRLAIYFIQSPL
                     PYDEEKKQWKVTGWYISSTDNQSSTKQIAFEGYTDTDNLSDSKFISSFKSWFESGKIK
                     NIANYNPKGERDGKYESYYNSNGNVYESLSFSNGLINGEFITYYENGAIESKRHFNND
                     KIKDGECPHFYEDGKIKQQHSYLNSKLDGPAYEYYPDGKLMQENFYQQSVLIGNDTSY
                     YQSGKISSIHNRNSRGQYDGANERYSEEGKLLSKSVYKDGKQISVQTWYENGQKEEEK
                     HFDEQGQLNGLVKQWYKNGNLAKSQNYKHDILDGNSEEWYENGIPESLYPYKNGKTDG
                     IAKSWNKYGKLTYSIEYKNGVENGAYRNWSKNTGKLTKETLYVNGIRQGVEKEFNDRT
                     GKLLTSTQYVNNKRHGTEETYDQNGIKYITCYQNDQKLSSLDNPTQIKDNATTGDSSA
                     QFALGKYEFICANIDEGIKWLTKSAEQKNTDAIYFLATAYKGNGIPANNEKYLAYLQQ
                     AATLGSSNAQAEIGYLYLIGKELPQNLPDAGVWFKKAAAQGNFAAHFYLGRMYQNGDG
                     VERNMEKARFHLSNAAEGGIEPALKALNELEHQTK"
     gene            2320..4050
                     /locus_tag="OO349_001376"
     CDS             2320..4050
                     /locus_tag="OO349_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5866511.1"
                     /translation="MKAKSTLLLMILSVFYSHQVLSSEPTQPDVYSVVEKKLNSALPL
                     EENTTFRSQAEWFNLQYELLVSGYPERAYNLLSKLETESKNAKYYLDLTASRVAEEEA
                     PETALTFLSKIGLNKPATLTSFESYINSWIKSKQPEKAMQLLSLDENALNYYLPTVLV
                     AYQIVPDKAISIYNSVYGDNVVIPYQQVTMLLTVAEGYQTKGDIKNAIHYADKALNMF
                     DKAIAVDSSRESHYSDEYLKLMNIYAANGNKEKAVVLSQRLHKAITESQSNYLSTLEG
                     ILLFYRNNDMQQDYQNSLSNYIVFIDKIFAFSPSTRSELSLINLLNSLNEVELMRKRL
                     AFLMTAPEYACYDSQYCYEDKVSALKILYRHHDNELMDKYFTTLLKEISNQPLEDWDI
                     IISNTGEKFSEVGLTKYAQHLASFAEEFYIKEQKSHSESEMRGLFSSLAELYSFGNDT
                     ASANRVFHQHVPAFNTDHMIQYFINAKEWNNARELLIKEEMLGMHNLILLENICMQKN
                     AECMAHITFTLNKLTTQPAITKIDSVGNEQLYQIGKIYHKLEIKPEPEQQVLIQSLYD
                     RASGSASATQ"
     gene            complement(4110..4601)
                     /gene="yajQ"
                     /locus_tag="OO349_001377"
     CDS             complement(4110..4601)
                     /gene="yajQ"
                     /locus_tag="OO349_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HCR5866512.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            4769..5680
                     /gene="panE"
                     /locus_tag="OO349_001378"
     CDS             4769..5680
                     /gene="panE"
                     /locus_tag="OO349_001378"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCR5866513.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            5643..6233
                     /gene="yajL"
                     /locus_tag="OO349_001379"
     CDS             5643..6233
                     /gene="yajL"
                     /locus_tag="OO349_001379"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HCR5866514.1"
                     /translation="MSASALVCLAPGSEETEAVTTINLLVRGGIKVTTASVDSDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGTECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(6287..7735)
                     /gene="thiI"
                     /locus_tag="OO349_001380"
     CDS             complement(6287..7735)
                     /gene="thiI"
                     /locus_tag="OO349_001380"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HCR5866515.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLTIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            7941..8183
                     /gene="xseB"
                     /locus_tag="OO349_001381"
     CDS             7941..8183
                     /gene="xseB"
                     /locus_tag="OO349_001381"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HCR5866516.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            8183..9082
                     /gene="ispA"
                     /locus_tag="OO349_001382"
     CDS             8183..9082
                     /gene="ispA"
                     /locus_tag="OO349_001382"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HCR5866517.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDANMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQALKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            9107..10969
                     /gene="dxs"
                     /locus_tag="OO349_001383"
     CDS             9107..10969
                     /gene="dxs"
                     /locus_tag="OO349_001383"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HCR5866518.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNCRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLVI
                     LNFGTLMPETAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            11024..11941
                     /gene="yajO"
                     /locus_tag="OO349_001384"
     CDS             11024..11941
                     /gene="yajO"
                     /locus_tag="OO349_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HCR5866519.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSINDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHCWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKP"
     gene            complement(12052..12570)
                     /gene="pgpA"
                     /locus_tag="OO349_001385"
     CDS             complement(12052..12570)
                     /gene="pgpA"
                     /locus_tag="OO349_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HCR5866520.1"
                     /translation="MTILSRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(12548..13525)
                     /gene="thiL"
                     /locus_tag="OO349_001386"
     CDS             complement(12548..13525)
                     /gene="thiL"
                     /locus_tag="OO349_001386"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HCR5866521.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFVTP"
     gene            complement(13603..14022)
                     /gene="nusB"
                     /locus_tag="OO349_001387"
     CDS             complement(13603..14022)
                     /gene="nusB"
                     /locus_tag="OO349_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HCR5866522.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(14042..14512)
                     /gene="ribE"
                     /locus_tag="OO349_001388"
     CDS             complement(14042..14512)
                     /gene="ribE"
                     /locus_tag="OO349_001388"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HCR5866523.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(14601..15704)
                     /gene="ribD"
                     /locus_tag="OO349_001389"
     CDS             complement(14601..15704)
                     /gene="ribD"
                     /locus_tag="OO349_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HCR5866524.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGVLLQAGLVDELIVYIAPKLLGSDARGLC
                     SLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(15708..16157)
                     /gene="nrdR"
                     /locus_tag="OO349_001390"
     CDS             complement(15708..16157)
                     /gene="nrdR"
                     /locus_tag="OO349_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HCR5866525.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDYVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            16308..16847
                     /locus_tag="OO349_001391"
     CDS             16308..16847
                     /locus_tag="OO349_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HCR5866526.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            17145..18029
                     /gene="tsx"
                     /locus_tag="OO349_001392"
     CDS             17145..18029
                     /gene="tsx"
                     /locus_tag="OO349_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HCR5866527.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(18206..18553)
                     /locus_tag="OO349_001393"
     CDS             complement(18206..18553)
                     /locus_tag="OO349_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HCR5866528.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(18682..19653)
                     /gene="secF"
                     /locus_tag="OO349_001394"
     CDS             complement(18682..19653)
                     /gene="secF"
                     /locus_tag="OO349_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HCR5866529.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALTHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(19664..21511)
                     /gene="secD"
                     /locus_tag="OO349_001395"
     CDS             complement(19664..21511)
                     /gene="secD"
                     /locus_tag="OO349_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HCR5866530.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(21539..21871)
                     /gene="yajC"
                     /locus_tag="OO349_001396"
     CDS             complement(21539..21871)
                     /gene="yajC"
                     /locus_tag="OO349_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HCR5866531.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(21894..23021)
                     /gene="tgt"
                     /locus_tag="OO349_001397"
     CDS             complement(21894..23021)
                     /gene="tgt"
                     /locus_tag="OO349_001397"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HCR5866532.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSIYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(23076..24146)
                     /gene="queA"
                     /locus_tag="OO349_001398"
     CDS             complement(23076..24146)
                     /gene="queA"
                     /locus_tag="OO349_001398"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HCR5866533.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            24239..24812
                     /gene="acpH"
                     /locus_tag="OO349_001399"
                     /pseudo
     CDS             24239..24812
                     /gene="acpH"
                     /locus_tag="OO349_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
     gene            complement(24817..26634)
                     /gene="malZ"
                     /locus_tag="OO349_001400"
     CDS             complement(24817..26634)
                     /gene="malZ"
                     /locus_tag="OO349_001400"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HCR5866534.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGVLLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNLGTGGACHNSES
                     PWRDWYSFSDDGTALDWLGYASLTKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNMQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYRRMIVLRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEASEV
                     VLPASPFLNAVQWQCKEGHGQLTDGILALPAISATVWMN"
     gene            complement(26790..28163)
                     /gene="proY"
                     /locus_tag="OO349_001401"
     CDS             complement(26790..28163)
                     /gene="proY"
                     /locus_tag="OO349_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HCR5866535.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVLFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(28239..29558)
                     /gene="brnQ"
                     /locus_tag="OO349_001402"
     CDS             complement(28239..29558)
                     /gene="brnQ"
                     /locus_tag="OO349_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HCR5866536.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVVAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFCLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(29965..31260)
                     /gene="phoR"
                     /locus_tag="OO349_001403"
     CDS             complement(29965..31260)
                     /gene="phoR"
                     /locus_tag="OO349_001403"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HCR5866537.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(31318..32007)
                     /gene="phoB"
                     /locus_tag="OO349_001404"
                     /pseudo
     CDS             complement(31318..32007)
                     /gene="phoB"
                     /locus_tag="OO349_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
     gene            32197..33399
                     /gene="sbcD"
                     /locus_tag="OO349_001405"
     CDS             32197..33399
                     /gene="sbcD"
                     /locus_tag="OO349_001405"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HCR5866538.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQMDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIISSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGSSKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLR
                     TLAGEHEA"
     gene            33396..36539
                     /gene="sbcC"
                     /locus_tag="OO349_001406"
     CDS             33396..36539
                     /gene="sbcC"
                     /locus_tag="OO349_001406"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HCR5866539.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVTLLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLIVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            36665..37846
                     /gene="araJ"
                     /locus_tag="OO349_001407"
     CDS             36665..37846
                     /gene="araJ"
                     /locus_tag="OO349_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HCR5866540.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMSVLTELAHNVGISIPAAGHMI
                     SYYALVVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQECWRYTFL
                     LIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAWF
                     SYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIVL
                     ALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGGAGGQIAFNL
                     GSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKPL
                     G"
     gene            complement(37989..38897)
                     /gene="mak"
                     /locus_tag="OO349_001408"
     CDS             complement(37989..38897)
                     /gene="mak"
                     /locus_tag="OO349_001408"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HCR5866541.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNVNSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVTIGTGCGAGVAFNGRTHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVSKAKHGDSSGVRGAAWLWPQE"
     gene            39022..39933
                     /gene="rdgC"
                     /locus_tag="OO349_001409"
     CDS             39022..39933
                     /gene="rdgC"
                     /locus_tag="OO349_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HCR5866542.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWLDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(40011..40466)
                     /locus_tag="OO349_001410"
     CDS             complement(40011..40466)
                     /locus_tag="OO349_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5866543.1"
                     /translation="MTQRPWSKLQREIYDLLTPTINLQIHCTRYPMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNAGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(40463..40798)
                     /locus_tag="OO349_001411"
                     /pseudo
     CDS             complement(40463..40798)
                     /locus_tag="OO349_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(40953..41237)
                     /gene="ppnP"
                     /locus_tag="OO349_001412"
     CDS             complement(40953..41237)
                     /gene="ppnP"
                     /locus_tag="OO349_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HCR5866544.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(41309..41986)
                     /locus_tag="OO349_001413"
     CDS             complement(41309..41986)
                     /locus_tag="OO349_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HCR5866545.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(42247..42435)
                     /gene="yaiA"
                     /locus_tag="OO349_001414"
     CDS             complement(42247..42435)
                     /gene="yaiA"
                     /locus_tag="OO349_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HCR5866546.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDK"
     gene            complement(42485..43009)
                     /gene="aroL"
                     /locus_tag="OO349_001415"
     CDS             complement(42485..43009)
                     /gene="aroL"
                     /locus_tag="OO349_001415"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HCR5866547.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAASTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(43192..43650)
                     /locus_tag="OO349_001416"
     CDS             complement(43192..43650)
                     /locus_tag="OO349_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HCR5866548.1"
                     /translation="MTIWVDADACPNVIKEILYLAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNSRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            43770..44579
                     /gene="proC"
                     /locus_tag="OO349_001417"
     CDS             43770..44579
                     /gene="proC"
                     /locus_tag="OO349_001417"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HCR5866549.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(44596..>45676)
                     /gene="adrA"
                     /locus_tag="OO349_001418"
                     /gene_synonym="dgcC"
     CDS             complement(44596..>45676)
                     /gene="adrA"
                     /locus_tag="OO349_001418"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HCR5866550.1"
                     /translation="KKAAAHGEEPPLTPQNEHQRSGLRFARRVRLPRAVGLAGMFLPI
                     ASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNLKTDAVLAGMWVG
                     VMGVNVLPSTAMLMILCLNLMGAGGPRLFVAGLVLMVVSCLVTLELTGITVSFNSAPL
                     EWWLSLPIIVVYPLMFGWVSYQTATKLAEHKRRLQVMSTRDGMTGVYNRRHWETMLRN
                     EFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQITLRGSDVIGRF
                     GGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVGVAPLNPQMSHYR
                     EWLKSADLALYKAKKAGRNRTEVAA"
BASE COUNT        11869 a        11919 c        11178 g        10710 t
ORIGIN      
        1 taagttggca gcattacctt cgcttaattc catataactg gacgattaca ttagaagtaa
       61 actatacttt ttatgaaata tcagggataa attattcaca tggaaaatac atcatgtaca
      121 aatcattatt tactattatc attctactgt atagcgccag ttcatatgca aaaagcgaat
      181 cccctcccgc aagcactata actttcattg cacaaatcga tcatgttgag ctaaataaaa
      241 cagcattatc tcatgatatg aaactcgttc ttgccgatcg tttttatttc aaaaccagta
      301 atccttgcga aacaaattca atttctgctc gaccagaatc aatggatatt actgacaaag
      361 cctatgccga caaattaaaa aaattagaaa ctccttttct tgatgatcgc tatttcttta
      421 tgaccatcaa tcagtgtgac ggtaatggca ctcccatgct gacagacata aaagtatgta
      481 caacagaatt atgtggtgct gaatatacaa agaaaatgtc tactgtatgg ctcgatgaaa
      541 ctttaaaacc aactgctaaa cgtctggcaa tttattttat tcaatctcct ttaccctatg
      601 acgaggagaa aaaacaatgg aaagtcaccg gatggtatat ctcatcaact gataatcagt
      661 cttcaactaa acagattgca tttgaaggat atacagacac agataacctc tccgattcta
      721 aatttatttc ttcatttaaa tcctggtttg aaagtggaaa gataaaaaac attgctaact
      781 ataatccaaa aggagaaaga gatggaaaat acgaaagtta ttacaatagc aatggtaacg
      841 tctatgaatc tctaagtttt tctaatggtc ttatcaacgg tgagtttatt acatattatg
      901 aaaatggggc cattgaaagt aaacgtcatt ttaataacga caaaattaaa gatggtgaat
      961 gccctcattt ttatgaagat gggaaaatca aacagcaaca tagctatctg aatagtaaac
     1021 tcgatggtcc tgcatatgaa tattatcctg atggcaaact catgcaggaa aatttctatc
     1081 agcaaagtgt gttgattgga aacgatacca gttactacca atccggaaaa ataagctcta
     1141 tacacaatag aaattcccgt ggtcagtatg atggagctaa cgaacgttat agtgaagagg
     1201 gtaagttact gtctaaatcc gtctataaag acggaaaaca aatttctgtt caaacctggt
     1261 atgaaaatgg gcagaaagaa gaggagaaac attttgatga gcaaggccaa ttaaatggtc
     1321 tggttaagca atggtataaa aatggtaatc ttgcaaaatc acaaaactat aaacatgaca
     1381 tactcgatgg taacagtgaa gagtggtacg aaaatggcat ccctgaaagt ttgtaccctt
     1441 ataagaatgg aaaaaccgac ggcattgcca aatcatggaa taagtatggt aaattaacct
     1501 attcaatcga atacaaaaat ggcgtagaga atggggcata tcgtaactgg agcaaaaaca
     1561 caggtaaact aaccaaagaa accctgtacg tcaatggtat cagacaggga gtggaaaaag
     1621 agtttaacga tcgcacaggt aaactattaa cctccacgca atatgttaat aataaaagac
     1681 atggtacgga ggaaacttat gatcaaaacg gaataaaata tattacatgc tatcaaaatg
     1741 atcagaaatt atcgtcttta gacaatccaa cacaaataaa agataatgca acaacaggcg
     1801 attccagtgc tcaatttgca ttagggaaat acgaattcat ctgtgcaaat atagatgaag
     1861 ggattaaatg gttaaccaaa tctgcagagc aaaaaaacac tgatgcgata tactttttag
     1921 ctacagccta caaaggcaat ggtatacctg caaacaatga aaaataccta gcatatctgc
     1981 aacaagctgc aacactgggc agtagtaatg cccaggcgga aattggctat ttatatctca
     2041 tcggaaaaga actaccacag aatcttcctg atgctggtgt ctggttcaaa aaagcagctg
     2101 cacaaggtaa ttttgccgca catttctatc tgggaaggat gtatcagaat ggtgacggcg
     2161 ttgagagaaa tatggaaaaa gccaggtttc atctatctaa tgcggcagaa ggtggaatag
     2221 aacctgcgct taaagcatta aatgaacttg aacatcaaac aaaataaagc gctcagggac
     2281 cacgtaaggt tcatcaaaca gaaaaggata tagtttgcga tgaaagcgaa atctactttg
     2341 ttattaatga ttttatctgt tttttattct catcaggttt tatccagcga acccactcag
     2401 ccggatgttt attctgtcgt cgagaaaaaa ctaaactctg ctttacctct ggaagaaaat
     2461 accaccttca gatcgcaggc tgaatggttt aatttgcaat atgaattatt agtatctggc
     2521 taccctgaac gtgcttacaa tttactgagc aaacttgaga cagaatcgaa aaacgctaaa
     2581 tattatctgg atttaactgc ctccagagtc gctgaagaag aagcaccaga gacagcctta
     2641 acttttttat caaaaattgg gttaaataaa ccggctactc ttacatcctt tgagagctat
     2701 atcaatagct ggataaaaag caaacaaccc gaaaaggcta tgcagcttct cagcctcgat
     2761 gagaatgccc tgaattatta tttaccgact gtgttagtcg catatcagat cgttcctgat
     2821 aaggcaataa gtatttataa ttctgtatat ggagataatg ttgttatacc ctatcagcaa
     2881 gtcaccatgt tacttacagt tgcggagggt tatcaaacaa aaggcgatat aaaaaacgcc
     2941 attcattatg ccgataaggc gctgaatatg tttgataaag ctattgctgt agatagcagt
     3001 cgtgaatccc attatagtga tgagtatttg aaactcatga atatctacgc cgccaatggc
     3061 aacaaagaaa aagcggtggt tttatctcaa cgtctacaca aagccattac cgaatctcaa
     3121 tcgaattact taagtactct ggaaggaatt ttattgtttt atagaaataa tgatatgcag
     3181 caagactatc aaaacagcct ttcaaattac atagtattta tcgataaaat ttttgccttt
     3241 tccccatcaa caagaagtga attaagcctc attaatttat taaattcatt aaatgaggtg
     3301 gaattgatga gaaaacgtct ggcgtttctg atgacagcac cagaatacgc ttgttatgat
     3361 agtcagtatt gttatgaaga caaagtcagc gcattaaaaa tcctgtatcg tcatcatgac
     3421 aatgagctaa tggacaaata tttcacgact cttcttaaag agatcagtaa tcaaccatta
     3481 gaagactggg atataataat aagtaatact ggtgaaaaat ttagtgaagt gggtctaacc
     3541 aaatatgctc aacatctcgc atctttcgct gaagaatttt atataaaaga gcaaaaatca
     3601 cattctgaaa gtgaaatgag aggactattt tcatcacttg ccgaattata tagttttggt
     3661 aatgatactg ccagcgcaaa ccgagtgttc catcagcatg tccctgcctt caatacagat
     3721 catatgattc aatattttat aaatgccaaa gaatggaaca atgccaggga attattgata
     3781 aaagaggaaa tgttgggtat gcataatctg atattgttgg agaatatttg tatgcaaaaa
     3841 aatgcagaat gcatggcaca cattaccttt acgcttaata aattaacaac acaaccagca
     3901 ataacaaaaa tagatagcgt gggtaatgag caactttatc agattggcaa aatctaccat
     3961 aagttggaaa ttaaacctga gccagagcaa caggttttaa ttcagtcgct ttatgaccgg
     4021 gcatcaggaa gcgcctctgc aactcaataa atgcatggtc aaaagggcca ccacggtggc
     4081 cctgacaatc agtagctata tccagaaagt taatcgcgga agtttttgaa ctggaacggc
     4141 tgaccgagat cgccaccgcg caccatcgcc atgacagcct gcaaatcatc acgagatttg
     4201 ccagtcacgc ggatctcatc gccctgaatt tgcgcctgca ctttcagttt gctgtctttg
     4261 atcatcttga cgattttttt ctgagtggcg ctctcaatgc cctgtttcag tttcgcttcc
     4321 acaaaccagg ttttaccgct atgaacgata ttttccggta catccagtga actgccttca
     4381 atgccgcgct tcagcagctt ggcacgcaga atatccagca actgattgac ctggaagtcg
     4441 gactcgctca acactttgat ggttttgctg gcgtcgttca gctcaaatga ggcttcaacg
     4501 ttacggaagt caaaacggga ctccacttcg cgactcgcgt tatcgaccgc gttacgtgct
     4561 tcctgaagat caacttcaga gacaatatcg aaagatggca tcttttcctc tcccttcatt
     4621 tttgatgcga agcataatac ctgcaaagtt aaataaccaa cagtcctgca ccaggacgta
     4681 tcccttaatt gtgcatagtc gcaacagcat gcacaattaa gggatagcct tattgaacac
     4741 ccggagtggt tgcgggtgag gaggaacaat gaaaattacc gtattgggat gcggtgcctt
     4801 agggcaatta tggcttacag cactttgtaa acagggtcat gaagttcagg gctggctgcg
     4861 tgtaccgcaa ccttattgta gtgtgaatct ggttgagaca gatggttcga tatttaacga
     4921 atcgctgacc gccaacgatc ccgattttct cgccaccagc gatctgctcc tggtgacgct
     4981 gaaagcatgg caggtttccg atgccgtcaa aagcctcgca tccacactgc ctgtaactac
     5041 gccaatactg ttaattcaca acggcatggg caccatcgaa gaattgcaaa acattcagca
     5101 gccattactg atgggcacca ccacccatgc cgcccgccgc gacggcaatg tcattattca
     5161 tgtggcaaac ggaatcacgc atatcggtcc ggcacggcaa caggatggcg attacagtta
     5221 tctggcggat attttgcaaa ccgtgttgcc tgacgttgcc tggcataaca atattcgcgc
     5281 cgagctgtgg cgcaagctgg ccgtcaactg cgtgattaat ccactgactg ccatctggaa
     5341 ttgcccgaac ggtgaattac gtcatcatcc gcaagaaatt atgcaaatat gcgaagaagt
     5401 cgcggcagtg atcgaacgcg aagggcatca tacttcagcg gaagatttgc gtgattacgt
     5461 gatgcaggtg attgatgcca cagcagaaaa tatctcgtcg atgttgcagg atatccgcgc
     5521 gctgcgccac accgaaatcg actatatcaa tggttttctc ttacgccgcg cccgcgcgca
     5581 tgggattgcc gtaccggaaa acacccgcct gtttgaaatg gtaaaaagaa aggagagtga
     5641 atatgagcgc atcggcactg gtttgcctcg cccctggtag tgaagagact gaagccgtca
     5701 ccacgatcaa cctcttggtt cgcggcggta tcaaagtcac cactgccagc gtcgacagcg
     5761 atggcaacct ggcaattacc tgctcgcgcg gcgtgaagct gctggcggat gcgccgctgg
     5821 tcgaagtggc tgatggcgaa tatgacgtga tcgtgctgcc tggtggcatt aaaggcacgg
     5881 agtgttttcg cgatagcact ctgctggttg aaaccgttaa acagttccac cgttccgggc
     5941 gtatcgtcgc ggctatttgc gccgcgccag ccaccgtgct ggtgccgcac gatatctttc
     6001 cgattggcaa tatgaccggc ttcccgacgc tgaaagacaa aattcccgct gaacaatggc
     6061 aggacaagcg cgtcgtctgg gatgcacggg taaaattgct gaccagccag gggccgggta
     6121 cagctatcga ctttggtctg aaaattatcg acctgctggt tgggcgcgag aaagcccatg
     6181 aagtggcatc acaactggtg atggcggcag ggatttataa ttattacgag tagtgtcgga
     6241 tgcggcaaac gtcgcatctg accagatgcg acgtaaaaac tacaaattac gggcgatata
     6301 ccttcacatt gttaaagcct tgctcgcgca gatagagcgc ctgcagacgg ctcatcaccc
     6361 cgcgctcaca ccacagcagc caggttttgt tctggtcgag atcgccaaat ttggtgctca
     6421 gtttatagaa cggcagagaa accacatcaa tcccttcgac tttcagtggc ttatcttcct
     6481 gttcatcgac agaacggata tcgagaatca cgtcgttcgg gccgaagccg ttgacggttt
     6541 ccacttccac cacttcctgc tcggtctgct gggcgatttc gcggatatca acgttattcg
     6601 cttcctcaac cactttatcg agaatactga agtcgaactt ctcttcttcc gcttcaatct
     6661 tcgatttaac cgctttcacc gtcgggcttt tggagatcac gccacaatat tccggcatcg
     6721 tgcgagcaaa gtcttcggtg ccaatctggc gagccaggtt gatgatgtgc tctttgtcgt
     6781 aagaaatcag cggacgcagg atcagcgtgt cagagacgtt atcaatcagg cgcaggttgg
     6841 tcagcgtctg actggacacc tggccgagcg cttcgccggt gaccagcgcc tgtacgccgt
     6901 aacgttcagc cactttagat gcggcacgca ccatcatacg tttgaggata acgcccatct
     6961 gaccgtcgtc gattttctcg agaatttccc cgacaaccgg ttcgaaatta atggcgacaa
     7021 aacgcacgcg gtgggagctg ccaaagcggt tccacagata atgcgccacc tgacgcacgc
     7081 caatttcatg cgctgcgccg ccgaggttaa agaagcagta atgcacgcgg cagccgcgac
     7141 gcatcaacat ataactggaa acaccggagt cgaaaccacc ggaaatgagc gacagcacat
     7201 cttcctgggt gccaatcggg aaaccaccaa taccttcgta gcgaccttta atcagcagga
     7261 ggcgatcgtc ttccacttcc agatgaacag ttacatccgg gttagtcagc ttcacgcgcg
     7321 cggattcaat atgctgattt aaaccgccgc cgacgtaacg ttccacatca atcgagctaa
     7381 aatcatgttt gccacggcgc ttcacgcgta cgcagaaggt tttgccttcc agctgatcgc
     7441 gatactgaac caacgctttc tcaaaaatat cgtgcatgtc ggtaaacggc acgtcttcga
     7501 cttcgagaat atggtggata cccggaatac gggtcagggc gtcgcgaata gtcagacgct
     7561 ggttttcatc tttcgcgcga acttcgatgt tatcccagtg gcggacgaca gcgagcgtct
     7621 catcatagtg ctttaaaacg ttacgaatgt tcccggtaag gatttttata aagcgcaagc
     7681 gcacagattg gcttttgatg gtgatttccg ggaacaattt aatgataaac ttcatggcgg
     7741 caatggttcg ttggcaagcc ttaagcgact tgtataggga aaaatacagc agcccacacc
     7801 tgcggctgca tccaggcgcg gaagtatacc actaacatcg ctttgctgcg cacatcacct
     7861 taccattgcg cgttatttgc tatttgccct gagtccgtta ccatgacggg gcgaaaaata
     7921 ttgagagtca gacattcatt atgccgaaga aaaatgaggc gcccgccagc tttgaaaagg
     7981 cgctgagcga gctggaacag attgtaaccc gtctggaaag tggcgacctg ccgctggaag
     8041 aggcgctgaa cgagttcgaa cgcggcgtgc agctggcacg tcaggggcag gccaaattac
     8101 aacaagccga acagcgcgta caaattctgc tgtctgacaa tgaagacgcc tctctaaccc
     8161 cttttacacc ggacaatgag taatggactt tccgcagcaa ctcgaagcct gcgttaagca
     8221 ggccaaccag gcgctgagcc gttttatcgc cccactgccc tttcagaaca ctcccgtggt
     8281 cgaaaccatg cagtatggcg cattattagg tggtaagcgc ctgcgacctt tcctggttta
     8341 tgccaccggt catatgtttg gcgttagcac aaacacgctg gacgcacccg ctgctgccgt
     8401 agagtgtatc cacgcttact cattaattca tgatgattta ccggcgatgg atgatgacga
     8461 tctgcgtcgc ggtttgccga cctgccatgt gaagtttggc gaagcaaacg cgattctcgc
     8521 tggcgacgct ttacaaacgc tggcgttctc gattctaagc gatgccaata tgccggaagt
     8581 gtcggatcgc gacagaattt cgatgatttc tgaactggcg agcgccagcg gtattgccgg
     8641 aatgtgcggt ggtcaggcat tagatttaga cgcggaaggc aaacacgtac ctctggacgc
     8701 gcttgagcgt attcatcgtc ataaaaccgg cgcattgatt cgcgccgccg ttcgccttgg
     8761 tgcattaagc gccggagata aagggcgtcg tgctctgcca gtactcgaca agtacgcaga
     8821 gagcatcggc cttgccttcc aggttcagga tgacatcctg gatgtggtag gagatactgc
     8881 aacgttggga aaacgccagg gtgccgacca gcaacttggt aaaagtacct accctgcact
     8941 tctgggtctt gagcaagccc ggaagaaagc ccgggatctg atcgacgatg cccgtcaggc
     9001 gctgaaacaa ctggctgaac agtcactcga tacctcggca ctggaagcgc tagcggacta
     9061 catcatccag cgtaataaat aaacaataag tattaatagg cccctgatga gttttgatat
     9121 tgccaaatac ccgaccctgg cactggtcga ctccacccag gagttacgac tgttgccgaa
     9181 agagagttta ccgaaactct gcgacgaact gcgccgctat ttactcgaca gcgtgagccg
     9241 ttccagcggg cacttcgcct ccgggctggg cacggtcgaa ctgaccgtgg cgctgcacta
     9301 tgtctacaac accccatttg accaattgat ttgggatgtg ggacatcagg cttatccgca
     9361 taaaattttg accggacgcc gcgacaaaat cggcaccatc cgtcagaaag gcggtctgca
     9421 cccgttcccg tggcgcggcg aaagcgaata tgacgtatta agcgtcgggc attcatcaac
     9481 ctccatcagt gccggaattg gtattgcggt tgctgccgag aaagaaggca aaaattgccg
     9541 caccgtctgt gtgattggcg acggcgcgat taccgctggc atggcgtttg aagcgatgaa
     9601 tcacgcgggc gatattcgcc ctgatatgct ggtggtcctc aacgacaatg aaatgtcgat
     9661 ttccgaaaat gttggcgcgc ttaacaacca tctggctcag ctgctttccg gtaagcttta
     9721 ctcttcactg cgcgaaggcg ggaaaaaagt tttctctggc gtgccgccca ttaaagagct
     9781 gctcaaacgt accgaagaac atattaaagg catggtggtg cctggcacgt tgtttgaaga
     9841 gctgggattt aactacatcg gcccggtgga cggtcacgat gtgctggggc ttatcaccac
     9901 gctaaagaac atgcgcgacc tgaaaggccc gcagttcctg catatcatga ccaaaaaagg
     9961 tcgtggttat gaaccggcag aaaaagaccc gatcactttc cacgccgtgc ctaaatttga
    10021 tccctccagc ggttgtttgc cgaaaagtag cggcggtttg ccgagctatt caaaaatctt
    10081 tggcgactgg ttgtgcgaaa cggcagcgaa agacaacaag ctgatggcga ttactccggc
    10141 gatgcgtgaa ggttccggca tggtcgagtt ttcacgtaaa ttcccggatc gttacttcga
    10201 cgtggctatt gccgagcaac acgcagtgac ctttgccgcc ggtctggcga ttggtggata
    10261 caaacccatt gtcgcgatct actccacctt cctgcaacgc gcctatgatc aggtgctgca
    10321 tgacgtggca attcaaaagc taccggtcct gttcgccatt gaccgcgcgg gcattgttgg
    10381 tgctgacggt caaactcacc agggcgcttt tgatctctct tacctgcgct gcataccaga
    10441 aatggtcatt atgaccccga gcgatgaaaa cgaatgtcgc cagatgctct ataccggcta
    10501 tcactataac gatggcccgt ccgcggtgcg ctacccgcgc ggcaacgcgg ttggcgtgga
    10561 actgacgccg ctggaaaaac taccaattgg caaaggcatt gtgaagcgtc gtggcgagaa
    10621 actggtgatc cttaactttg gtacgctgat gccagaaacg gccaaagtcg ccgaatcgct
    10681 gaacgctacg ctggtcgata tgcgttttgt gaaaccactt gatgaagcgt taattctgga
    10741 aatggccgcc agccatgaag cgctggtcac cgtagaagaa aacgccatta tgggcggcgc
    10801 aggtagcgga gtgaacgaag tgctgatggc ccatcgtaaa ccagtacccg tgctgaacat
    10861 tggcctgccg gacttcttta ttccgcaagg aactcaggaa gaaatgcgcg ccgaactcgg
    10921 cctcgatgcc gccggtatgg aagccaaaat caaggcctgg ctggcataat ccctactcca
    10981 ctcctgctat gcttaagaaa ttattcatgg caggagtggc agcatgcaat acaacccctt
    11041 aggaaaaacc gaccttcgcg tttcccgact ttgcctcggc tgtatgacct ttggcgagcc
    11101 agatcgcggt aatcacgcat ggacactgcc ggaagagagc agccgtccca taattaaacg
    11161 cgcgctggaa ggtggcataa atttctttga taccgccaat agctattccg atggtagcag
    11221 cgaagagatc gttggtcgcg cactgcggga tttcgcccgt cgtgaagacg tagtcgttgc
    11281 caccaaagtg ttccatcgcg ttggtgattt accggaagga ttatcccgtg cacaaatttt
    11341 gcgctctatc aacgacagcc tgcgacgtct cggcatggat tatgtcgata tcctgcaaat
    11401 tcatcgctgg gattacaaca cgccgatcga agagacgctg gaagccctga acgacgtggt
    11461 aaaagccggg aaagcgcgtt atatcggcgc gtcatccatg cacgcttcgc agtttgctca
    11521 ggcgctggaa ctacaaaaac agcactgctg ggcgcagttt gtcagtatgc aggatcacta
    11581 taatctgatt tatcgcgaag aagagcgcga gatgctgcct ctgtgttatc aggagggcgt
    11641 ggcggtgatt ccgtggagcc cgctggcgcg ggggcgactg acgcgtccat ggggagaaac
    11701 taccgcacga ctggtgtctg atgaggtggg gaaaaatctc tataaagaaa gcgatgaaaa
    11761 tgacgcgcag atcgcagagc ggttaacggg cgtcagtgaa gaactcggtg caacacgagc
    11821 acaagttgcg ctggcctggt tgttaagtaa accgggcatt gccgcaccga ttatcggaac
    11881 ttcgcgggaa gaacagcttg atgagctgct gaacgcggtg gatatcactt tgaagccata
    11941 acagattgcc gagctggaaa cgccgtataa accgcatgcg gtcgtaggat ttaaataaga
    12001 gtccaggcct gatgagacgt gacaagcgtc acatcaggca tcggtgcaca actacgacag
    12061 aatacccagc ggccagtgat gaccgataaa gtacaggatg cctgcggaaa tcaccccggc
    12121 gacaatatcg tcgatcatga tccccatgcc gccatgtaca ttgcgatcaa accagcggat
    12181 cggccacggc ttccacatat ccagaatacg gaaaatcaca aacccggcgg caacccactg
    12241 ccagtcattg gtcggcagcg ccatgagcgt gatccacata ccaataaatt cgtcccagac
    12301 aatgctgccg tgatcgtgca cgcccatatc tttcgccgtt tgatgacaaa gatagacgcc
    12361 gatacagatc cccaacatca ccaccagcga gtagagctgc cagggtaaaa aggtcatcag
    12421 ataccagaaa gggatcgccg ccagcgatcc catcgtgcca ggaacgattg ggcttaatcc
    12481 acttccgaat ccgacagcaa gcagatgcca cggattactc atcttcaggc gacttttcgc
    12541 gacatcttta tggcgtgaca aaatggtcat atcctttcca gtcaaacgtg acaggttcgc
    12601 cgtcacgaat aaaacaaagc ccttcgatat cggcggtcat ttgcccgata caggtaaacg
    12661 gtacgccaag gtgtccgaga gcaacatcca gcgcgccacg gttcagttcc ggcaccgtga
    12721 aacacaactc gtaatcttca ccgccagaga gcgcccagcg cagcgcctgt tccggttcaa
    12781 catggcgaga aagcgcatca gaaaacggca gcaatgccag gtcaatacgt gcgccgcagt
    12841 cgctggcttt cacgatatgc ccgagatcgg aaatcagacc gtcagagaga tcgatagctg
    12901 aatttgccag atcgcgcagt gcctgcccct gtaaaatacg cggcgatgga cggagatgac
    12961 gtttgatcaa gtagtcagca tctttagcat cggcaacctg caaacggttt tgcaaaatcg
    13021 ccagcccggc ggcgctatcg cccggtgtac cggtcacata gatccagtca cccggtttcg
    13081 ccccagagcg cgttaaggct cgtcccatcg gaacaaagcc gtggataccc aacgtcattg
    13141 ataatggccc acgcgtggta tcgccgccaa tgagttgcat atcgtaataa ttgagaagat
    13201 caaacaaact gtcgctgaag gactcaagcc acgcttcgtc tacgtccggt aaggttaatg
    13261 ccagcgtcag ccaggccgga tcggccccca tcgctgccag atcgcttagg ttcaccgcca
    13321 gtgctttata agccagatca gcaggatcga tatcagggag gaaatggttt cccgccacca
    13381 gcgtatcagt gctgatcgcc agggtctgtt tctcggggat attgagaaga gcgcaatcgt
    13441 cgccgatgcc cagttcgaca tcaagacgag aacttcttac acggtcaaaa taacgggcaa
    13501 tcagggagaa ctcgccacat gccatacgtt atgcctcagc aggtaaaaaa gaaaaggccg
    13561 gcgatcatgg aacagtcttc cgtgaatcta ccggcctgga tattactttt tgttagggcg
    13621 aatcacaggt gctgctttat cgagtacgcc gttgacgaac ttatggctgt cttctgcgcc
    13681 gaacgatttc gccagttcga tcgcttcgtt aatggccact ttgtatggca catcactacg
    13741 tttagacagt tcgtacagcg caatgcgcag tactgctttt tctacctggc ccagttcttc
    13801 cagcaggcgg gacaggtagg gcttcatcag tccgtcgagg tatgcggtgt tagtcgccac
    13861 cccggccagc agctcacgga agtacaggac gtcaacgtct tttacatcct gttcagccag
    13921 gaactggtat tcaacatcag cgatgtcgtt ctgggacaac tgccaggagt agagcgcctg
    13981 gacggcacac tcacgagcgc ggcgacgagc agcaggtttc acggatttcc ccttactaat
    14041 ttcaggcctt gatggctttc aatacattaa tcatttcaag cgcggtcagt gcagcttctg
    14101 cacctttgtt gccagctttg gtgccagcac gttcgatcgc ttgttcaatg ctttcagtgg
    14161 tcagaacccc aaaagcaacc ggaatttcgc tgtcctgggc aacatgcgcc aggccgttgc
    14221 ttgcaccacc agcgacatat tcaaagtggg cagtgccacc acgaataacc gtacccagcg
    14281 caatcaccgc gtcgtattta ccggttttag ccagtgcacc cgccgccagc ggcagctcat
    14341 aggcaccagg cacccaaaca acggtaatgt tttcatcttt tacctgaccg atacgtttca
    14401 gtgcgtcaat tgcaccttcc agcaggctgt cattgataaa gttgttgaaa cgcgcgatgg
    14461 tgatggcgac gcgagcgtcc ggggtagcaa cgttagcttc aataatgttc attttcttcc
    14521 ttcgggttcg agtatggccc cgcagggggg cggattttag cataatattt cgtgcgctgc
    14581 ttccctttcg agccgggaga tcatgcaccc actaaatgca ggcaaacatc cgggcctaca
    14641 tgacgtatct ctttgaattt aaattggggg gcgtcggcta atttctcaag ccctggcagc
    14701 gagcataatc cacgggcgtc gctgcctaat agtttaggtg cgatatagac aatcagctca
    14761 tcgactaaac ccgcctgtag caatacgcca gcgagcgttg gccccgcttc cacccagatg
    14821 ctgttaatct gctgtttacc cagttgcatc atcagtacaa ccagatccag atgaccttta
    14881 tgctctggaa tcagcaaggt acgcaccgtt tccggccact cacgagaatc ttcctgcgta
    14941 cgcgcgaacc aggtttcgcc gggctgctgc acaatgcgat gttccggcgt cacgcgattt
    15001 tggctatcaa tcacaatacg tactggctgg cggagatttt gttgcgggta gagcgcctga
    15061 gtttgttcat ccagttcaga ccaacgcact gttaaggctg gatcatccgc cagcaccgtg
    15121 gcgctgctgg ttaaaatggc atgactttgt gcgcgctgtc gttgtacgtc gcgccgcgcc
    15181 tgaggcgaag tgatccactg gctttcgccg ctcgccatcg ccgtgcggcc atcaagcgat
    15241 gcgccaagtt ttaactgaat ataaggaaag ccggtgcgca tccgcttgag aaagccttta
    15301 ttcaattgct cggcttcact catcatcagc ccgtggctga cgtcaatgcc agcctgttgc
    15361 agacggtaaa gtccacgccc agcgacctgc gggttaggat cttgcatcgc tgcgaccacg
    15421 cgcgctacgc cagcggcgat gagtgcgtca cagcacggtg gcgtacggcc atgatggcta
    15481 cagggttcga gtgtcacata cgccgttgcg cctttggctt tttcacccgc catacgcaac
    15541 gcgtgtactt cggcatgtgg ttcacccgct cgttggtggt aaccttcacc gacaatttcg
    15601 ccatctttga caatgacgca cccgacattc gggttggggt gggtggtaaa acgtcctcgt
    15661 tgcgccagct ttagcgcccg cgccatgtaa tactcgtcct gcacggctta gtcctccagg
    15721 cgcgcgatct cttcgccaaa ttctttgata tcttcgaaac tgcggtagac agaggcaaaa
    15781 cggatatagg cgactttatc gagctttttc aattgctcca tcaccagatt gccaatcatc
    15841 ttgctcggta cttcgcgctc accggtggcg cgcagctgcg atttaatatg attgattgcc
    15901 atttcaacgt aatcggaact caccggacgt ttttccagcg cccgcagcat tccgctacgc
    15961 aatttctctt cattaaacgg ttcacgcacg tcgttgcttt ttacaacacg cggcataacc
    16021 agctccgcca cttcaaaggt ggtgaaacgt tcattacaca ccagacactg ccgacggcgg
    16081 cgtacggatg aaccctcgcc cacgagacga gagtcaatta ccttagtgtc cacggcgaaa
    16141 cagaatgggc aatgcatacg gcgtcctgac caggtggtta acggaaatct attttaccct
    16201 gaactgacga tacaacaaag gcgcaacgct tttgagataa aggatcgatg ctttcgctgc
    16261 ttttaccctc taccattagg ctaattgcga cgagaaaggg acgacctatg aatactaacg
    16321 tttttcgact gctcctgctg ggaagcctgt tcagccttag cgcctgtgtg cagcaaagtg
    16381 aagtgcgaca gatgaaacac agcgtcagca cgctgaacca ggagatgacg caactcaatc
    16441 aagaaacagt caaaatcact cagcaaaaca ggctgaatgc aaaatccagc agtggggttt
    16501 accttctgcc tggagcgaaa acaccggcaa gactggaaag ccagatcggt actttacgta
    16561 tgtcgctggt gaatattacg cctgatacag atggcaccac ccttacacta cgtattcagg
    16621 gtgagtcgaa tgatcccttg cccgcattca gcggaactgt tgaatatggg caaattcagg
    16681 gaacaataga caattttcag gaaatcaatg tgcagaatca attgattaat gcccctgcca
    16741 gcgtcctcgc ccccagcgat gttgatattc cgttacaatt aaaggggatt tctgtggatc
    16801 agttaggctt tgtacgtatc cacgacattc aacctgttat gcagtaaacg tatttcggga
    16861 cgattttgtg cgtcccgcaa catcttcccc gtcattttgt tactctgctt acatcacctg
    16921 gattgatagt aaaagtttgc aacaagggcg aaagtcagta caatccccgc ccgaatgtgt
    16981 gtaaacgtga acgcaatcga ttacgtaaat gatagaactg tgaaacgaaa catatttttg
    17041 tgagcaatga tttttataat aggctcctct gtatacgaaa tatttagaaa cgcaatttgc
    17101 gcctttttca ctcccgcaag ggattttcaa acagtggcat acatatgaaa aaaacattac
    17161 tggcagccgg tgcggtactg gcgctctctt cgtcttttac tgtcaacgca gctgaaaacg
    17221 acaaaccgca gtatctttcc gactggtggc accagagcgt taacgttgtc ggaagctatc
    17281 acacccgttt cggaccgcag atccgcaacg atacctacct tgagtacgaa gcattcgcta
    17341 aaaaagactg gttcgacttc tatggttatg cggatgcgcc ggtattcttc ggcggtaact
    17401 ccgatgctaa aggtatctgg aaccacggtt ctccgctgtt tatggaaatc gaaccacgtt
    17461 tctccatcga caagctgacc aatactgacc ttagcttcgg tccgttcaaa gagtggtact
    17521 tcgcgaacaa ctacatttac gacatgggtc gtaataaaga tggtcgccag agcacctggt
    17581 acatgggtct gggtaccgat atcgacactg gcctgccgat gagcctgtcc atgaacgtct
    17641 atgcgaaata ccagtggcag aactatggcg cagcgaacga aaacgagtgg gacggttacc
    17701 gtttcaaaat taaatacttt gtgccgatta ccgatctgtg gggcggtcag ctgagctaca
    17761 tcggcttcac caacttcgac tggggttccg atttagggga tgacagcggt aacgcaatca
    17821 acggtattaa gacccgtact aataactcta tcgcttccag ccatattctg gctctgaact
    17881 acgatcactg gcactactct gtcgtagctc gttactggca cgacggtggt cagtggaacg
    17941 acgatgcaga actgaacttc ggcaacggca acttcaacgt tcgctctacc ggctggggtg
    18001 gttacctggt agtaggttac aacttctgat tatgaaaatg ccgggattta ttcccggcat
    18061 ttctgattgt taacaacgtg tgctttgttc atgccggatg cggcgtaaac gctttgtccg
    18121 gcccacaaaa gcacgtaaat tcaatatatt gctgaaataa cgtaggcctg ataagcgtag
    18181 cgcatcaggc aattttacgt tttaatcact tcttcttgtt catcatcgct ttcaggtcag
    18241 cgaatgggtt gtacttcgct tcaccgacat ctttctgcgc atcttcccct gcgataacgg
    18301 tcgtaccata ctgatccgct tcggtatatt tcgaatgctc atgatcgtgg caatagagac
    18361 acaacaattc ccagttactg ccatcttccg ggttattggt atggtcgtga tcaatgtggt
    18421 gaacggtaag ttcacgcagg ttagaataaa caaactcgcg ggaacagcga ccgcagaccc
    18481 acggatagat ttttaatgct ttttcgcgat agccgctttc taaccgtgca tagttttttg
    18541 ggatgatagc catgaaaagt tacctggatg aaatgtaggg ttacgcgcag tttcccaaga
    18601 tgcggggatt ttcgcaatct ccatacatca aaaaaaatcc cgatcttctg accgggattt
    18661 tcaacatcaa cgggaacttg attaaggcag aattgacggc tgatccgccc cttctttttc
    18721 cactttctgc tgcaacatgt gttcgcgctt catacccagt ttcagagcca acgcagatgc
    18781 tacgtagatg gaagatgcag tgccgatgga aacaccgata agcatggtca gcgagaagcc
    18841 ttccagtacc ggaccaccga agagatacag catcaggata accatcaagg tagtaccgga
    18901 tgtgatcaag gtacggtgca gcgtctgggt caaggacacg ttaaagattt cgtaaggcgt
    18961 accgcgacgg atcttgcgga agttttcacg aattcggtcc gataccacga tactgtcgtt
    19021 aagcgagtaa ccgataaccg acatcaacga tgccacaatg gtcaggtcaa tctcgatatg
    19081 gaataacgac aaaataccca gcgtaatgat aacgtcgtgc gtcagcgcaa taaccacccc
    19141 tgccgccagt cgccactcaa agcggaaacc tacgtacacg aggatagaca gcagcgctgc
    19201 catcaacgcc atcgcaccgg tttgcgcaag gtcagccccc acgctcggac cgacgaactc
    19261 aatacgcttc actgctgcat tctgattggt ggattcgtta atcaccttca gaacttggct
    19321 gcccagcacc tgaccgccgg tttcgccttc agcaggcggc atacggacca tgatgtcgtg
    19381 gctgctacca aagttttgca gcattggctc ttcaaaaccg gctttttgca atgcatcacg
    19441 cattacgtca atttcagccg gtttttcgag cgtaatttcg ataaccgtac caccggtgaa
    19501 atccagcccc cagttaaagc cgcgcacgcc cataataacg atagcagcga ttaacagcag
    19561 accagagatg ccgaaagccc agtagtccca gcgcataaag tcatagactt tacggccgtg
    19621 gtttagttgt tcaacagtat attcctgtgc cacatcgcac tcctcagatt gacagcttct
    19681 tgacgcgctt gccgccatat agcaggttta cgatggcacg cgtaccgaca atcgcggtaa
    19741 acatcgacgt cgccacaccg ataccggtag taatcgcgaa ccctttaatt gccccggtac
    19801 ccactgcgta caggatgatg actttaatca gcgtggtgat gttcgcatcg aagatagaac
    19861 tgaatgcgcc acgataacct tcatcaattg cctgttgaac agtacgtccg ttgctcaact
    19921 cttctttaat acgttcgttg atcagtacgt tcgcatcgac cgctaccgca agggttaaga
    19981 cgatacccgc gatacctggc atactcagtg ttgcgcctgg caacagcgac ataataccga
    20041 cgatcaagat caagttggca atcagagcac tggtcgcaat cagaccaaac ttcttataga
    20101 agatgatcat gaacagaata gaaaccagca gaccggcaag gcaagcctcc agcccctgct
    20161 caatgttctg catacccaag gtcgggccaa ttgtacgttc ttcaacaatc tgaatcggcg
    20221 cgatcaacgc accggcacgc agcagcagtg acagctgacg ggcttcgttc gggttgttga
    20281 tgccggtgat acggaagctg ttacccagac gagactggat gttggcgatg ttaatcacct
    20341 cttcctgttt caccagaacc gcacgaccat ttgcatcttt cttaccgctg tctttgtact
    20401 ccacaaacag ggttgccatc ggtttgccga tgttgtcctt ggtgaagtta gacataatgt
    20461 tgccaccagc gctatcgagc gagatgttaa cctgcggctg gttgtattcg tcctggctgg
    20521 aagtggagtc ggtgatatgg tcaccagtca gaattacgcg tttgtacagc acaaccggct
    20581 gaccttcgcg ggtctgtttc acttcagagt cgcccggtac gcgaccggat gccgcagcgg
    20641 cctgatcaac attggtgttt accagacgga attccagcgt tgccgtcgcg cccagaatct
    20701 ctttcgcacg cgcagtgtcc tgaataccgg gcagttcaac aacgatacgg tcagcaccct
    20761 gacgctgaac caccggctcc gccacgccaa gttggtttac acggttacgc aggatattaa
    20821 tgttctgctg caccgcatat tcacgcgctt cactcagacg agcatcgctc attaccgcac
    20881 gcagctggtt gctgccctgg ctgctaatca ccaggtccgg atggcgcttg ctcagatacg
    20941 caatggcttc atcacgagct ttagcatcgc ggaaagtgat gctcaggccg tagttgtttt
    21001 ctttacgaac agtggtatac gggatgccct tttcgcgcag gtcactgcgt aggctatcga
    21061 tattttgttc ctggagtttg ccaagcgcgg tgtccatatc cacttccatc aggaagtgaa
    21121 cgccgccacg caggtcaagg ccgagcttca tcggctcagc atgaatagct gccagccagc
    21181 gcggcgttgc cggggcaagg ttaagcgcca cgacgtattt gtcacccata acgcccatta
    21241 atgcttcacg agcgcgcaac tgggtgtcag tggagtcgaa gcgcgcaaga atagcgccct
    21301 cttccagtgc cacagactta gcagttattt tttcttcttg taacgttttc tggacctgga
    21361 tcagcgtttg ctcactggcg gcgactccgc gcgcaccagt gatctgaaca gccggatcct
    21421 caccaaacag gttgggaagc gcatacagca gaccgatgac aatcaccacg atcagcatga
    21481 cgtacttcca caaaggataa cggtttaaca cggcaattcc cttagggaaa aattttaatt
    21541 acagcgcctt catggtgcct ttcggcagga cggcagctac gaagtcacgt ttaataacta
    21601 cttcagtggt gtcattcagc gcgatagcaa tgtagccgtt ttccgctact ttggttacgc
    21661 gaccaaccag gccaccgttc gtcagaactt catcaccttt ggcaatggag tccatcagct
    21721 ttttgtgttc tttggtgcgc ttctgctgtg gacgcaggat catgaaatag aaaatcagac
    21781 cgaataccac cagcatcaaa atcaaagaca tcgggctacc ttgcgccggt gcacccgttg
    21841 ccgctaccgc atcagaaata aaaaagctca ttaaatttcc ctcattatta atattaatca
    21901 acgttcaaag gtggtacttc tcgcccctga cgctggtaaa aatcagttac gaagctctct
    21961 aatttaccct cttcaatagc cttgcgtaaa cccgccatca aacgctggta gtaacgaagg
    22021 ttatgaatgg tgttgagtcg cgcgcctaat atttcgttgc aacggtcgag atgatgcaag
    22081 taagcgcgtg aataattgcg acaggtgtag caatcacact caggatcgag tgggccagta
    22141 tcgctcttat atttcgcatt gcggattttc accacgccat cggtcacgaa caaatgacca
    22201 tttcgggcgt tgcgggttgg cattacgcag tcaaacatat cgataccgcg acgtacgcct
    22261 tcaaccaggt cttccggttt accaacgccc atcaggtaac gcggtttgtc tgccggaatt
    22321 tgcggacaca catgctccag aatacggtgc atatctgctt tcggctcacc cacagccaga
    22381 ccgccgacag cgtagccatc aaagccgata tctaccagac ctttaacaga tatatcacgt
    22441 aaatcttcgt aaatactgcc ctgaattata ccaaataacg catttttgtt accaagactg
    22501 tcaaaacgct cacggctacg cttcgcccaa cgcagagaca tctccataga gcgttttgcg
    22561 taatcccagt cagcaggata cggcgtacac tcatcaaaga tcatgacgat atccgaacca
    22621 agatcgtact gaatctccat cgacttttcc gggtcgagga aaatcgggtc gccgttgatc
    22681 gggttacgga agtgaacgcc ctgttcggtg attttacgaa tatcaccaag gctgaagacc
    22741 tggaagccgc cggagtcggt aagaatcggt cctttccact gcataaaatc gtgcagatcg
    22801 ccgtgcagtt tcatgatttc ctggcccggg cgcagccaca ggtggaaggt gttgccgagg
    22861 ataatttgcg cgccagtggc ttcaacttct tccggcgtca tcccttttac ggtgccgtag
    22921 gtgccaacag gcataaaaca aggcgtttcc actacgccac gatcaaagac caggcggcca
    22981 cggcgtgcgc gaccgtcggt ggtgtccagt tcaaatttca ttttttctcc actacgtcag
    23041 aaaaacagtc caacgtttaa accagcgccg cggaattact ccccgacgcg ctcattaatt
    23101 gcctgcggat tgtacgtgat aaacatcgca tcaccgtaac taaaaaagcg atatttctct
    23161 tctaccgccg ctttataggc attcatggtg tgttgataac cggcaaaggc cgaaaccagc
    23221 ataatcagcg tcgactctgg caagtggaag ttcgtcacca gcgcatcgac cactttgtac
    23281 tggaagcccg gatagataaa gatttgggta tcgtcaaaga acggttcgat aagatcgttt
    23341 tttgccgcct gtgccgcgct ttccagcgaa cgtactgaag tggtgccaac cgcaatcacc
    23401 cggttaccgc gcgctttcgc cgccagtacc gcgtctacca catcctgcgg tacttcagcg
    23461 tattccgagt gcatgatgtg atcttcaatg gtgtcgacgc gcaccggctg gaaggtgccc
    23521 gcaccaacgt gcaacgtcac aaacgccatc tccacgcctt tggcgcgtaa tttttccagc
    23581 aaaggctcgt caaaatgcag accagcggtc ggggctgcaa ccgcgcccgg tttttcgcta
    23641 taaacggttt gataaagttc gcggtcagcg tcttcgtccg gacgatcgat atacggcggc
    23701 agcggcatat ggccgatgct gttgaggata tccagtaccg agcgttcatc attaaattcg
    23761 acttcaaaca gtgcgccgtg gcgcgcggtc attgttgcgt tgatactttc gtcatcgccc
    23821 agcaacagtt ctgcgccagg ttttggcgct ttcgaggcgc gaatatgcgc aagaatgcgt
    23881 ttgtcgtcga gcatccgctc aaccagcact tcaatcttgc cgccgctggc tttacgccca
    23941 aacagacgcg ccgggatcac gcgggtatta ttaaaaacca gaagatcgcc ggggttgagc
    24001 ttatcgagta aatcggtgaa agtaccgtgc gtcagcgcgc ccgtcggccc gtccagcgac
    24061 agtaaacgac agctactgcg ttcaggcatg ggatagtggg caatcaggga ttcgggcaat
    24121 tcaaaggaga aatcggtaac gcgcatgaca ctgactcaga ctaaaataag agtcgggtag
    24181 tctagtgccg gggcgctctc cctgcaacct ttacccccct ttggatagaa tataatcgat
    24241 gaatttttta gctcacctgc atttagccca tctcgcggaa agctcgcttt ctggcaattt
    24301 actggctgat ttcgtacgcg gaaatcctga agaaagtttt ccgcccgacg tcgtggctgg
    24361 cattcatatg catcgacgta ttgactgaca atctgccgga agtccgcgaa gcacaggagt
    24421 ggtttcgtag tgaaacgcgc cgcgttgcgc ctattacgct ggatgtcatg tgggatcact
    24481 ttctttcccg ccactggtcg cagctgtcgc cggactttcc gctacaggaa tttgtctgtt
    24541 atgcccgcga gcaagtgatg acgattttgc cggactcacc gccacgtttt atcaatctga
    24601 acaattactt gtggtcagag cagtggctgg tgcgctatcg cgatatggat ttcatccaga
    24661 acgtgttaaa cggcatggca agccgccgcc cacgtctgga tgccctgcgt gactcctggt
    24721 acgatttaga cgctcattat gacgccctcg aaacccgctt ctggcagttt tatccgcgga
    24781 tgatggcgca ggcgtcacac aaggcgttat aaaacgttag ttcatccata ccgtagccga
    24841 aatggcaggc aaagccagaa tcccgtcagt cagttgccca tgcccttctt tgcattgcca
    24901 ttgcacggca ttgagaaacg gtgacgcggg tagcaccact tcactggcct cgccacggtt
    24961 gattgccacc agtacacgtt gctgattcag cacgcggaca aataccacta cgttatcttc
    25021 cgcatacagc acctgacagc cgccacgacg cagcgcctga cttttcttac gcagcacaat
    25081 cattcgccgg tacagcgcga ataacgccgt atcctgcttt tccacctgcc aggggaacgg
    25141 tttacggcaa aacggatcgt ttttaccatc cagtcccact tcatcaccgt aataaatgca
    25201 cggtacacca ggccaggtga acagccagac caccgccagc ggcaggcgcg caatatcccg
    25261 accgagcagc gttttaaatc gcgcagtatc gtggctatcg agctgattaa acatacgtaa
    25321 ttgttgttga tgagaaagcc ctgcgcggta attatccatc caggccatac aggtttgggc
    25381 gtcaatttgc tgcggatcat aagagatgtc ggtattggca agaaatcccc acaacgggaa
    25441 tgtgaagcca cgatagttca tggcagcatc ttccacatcg gcctgtaacc attgccgtgc
    25501 atcgccaaaa tgttcgccga caatatacgc ttccggctgg gtttctttcg ccgcttcggt
    25561 gatcccggca acgtgctgca tattattgcg cgccccaccc gcctccccca gcatatgcac
    25621 cacatccagc cgccagccgt ccatattcca cggcgctttc agccagtggc ggacaatact
    25681 gtcttccccg cgataaattt cattcaccag actttccgac tgataatcca gcttcgtcaa
    25741 gctggcatag ccaagccagt cgagcgccgt gccatcatca ctaaacgagt accagtcgcg
    25801 ccagggcgat tcagagttgt gacaagctcc ccccgtgcca agattgtgcc tgtcaaacca
    25861 ggcatgggaa tcgccactgt ggttaaacac gccgtccagc accagccgca ttcccagctg
    25921 ctgcgtattg tgtcgcaaac gcagcaacac cccatcaccg ccaaactgcg gatcgacatg
    25981 gcgataatcc tcggtatcgt atttatgtac gctgggagct ttaaacaccg gattgagata
    26041 cagcgctgtc acgccaagct ttttcagata cggcagtttt tcgcttatcc cgtccagatc
    26101 gccgccatag aacgttgatc cgcccgcctg cgccgtgacc ggttcatccc agtcacgcaa
    26161 gatgatctct tgtccggctg catgatggta atagacatga tcctgttcag cttcacgagg
    26221 aagactacgc gcaaaacgat cagggaagat ctgataaaaa atctgatccg cagcccattg
    26281 tgggccgata tcaggtacat cgacggcaaa ctgctccagt cgtgccggcg gcattcggct
    26341 gaagccctgc ggtgtaaacc aacgctggcg atcgtgccac agcagtttga aactgtaacg
    26401 ccgccggggt tgtccgctgg agagatcaat cgccgcacgc catgcggtga cgcctggctg
    26461 cggctgactg cgctgcttat gcattggtac tgacatttct tcgttatcgt gttctgtacg
    26521 cagcataatg cgctgcggtg ggtcttcgcc cgtcagccac agtgtaatga gcagttgatc
    26581 tttgctttgt ttaacaaatg ggggcaccgg caggtgccat gcatttaaca tcataattcc
    26641 cctgtgatga aaatctgatc accttgccat agcctaatgc aggatcactc atcatctgaa
    26701 ggatatttgg gggaggagga cgggggcgga taactttcgg aatagccgga tcaggatttc
    26761 ctcctgaacc ggcttacaga acgcaagtat tattgatttt cagccagctg acgatcgtgg
    26821 cggcgtttaa acatccagcc aatcaacagc acaacaatcc acgcgaaacc gacatacagc
    26881 gagatacgcg tatccgggtg ataaccaatc aacccgataa taaagagcag gaaaatcagc
    26941 ccgccgatgg tcgttgctac cccacccggc actttaaatt tcagcgcctt aacttcttct
    27001 ggcggcaaac ggcgacggaa ggcgatttgc gacagcagga tcataatcca cacccatacc
    27061 gtggcgaagg ttgccagcga agcgatcacc aggaagacgt tttccggcat gatgtagttc
    27121 agatacaccg caaacagcag cgcggtagtc atcaccagca ccgtaaccca cggaataccg
    27181 cgacgcgacg ttttgctgaa aattttcggt gcgctgccct gctctgccat accgtggagc
    27241 atacggccta cgccaaatac gtcactgtta attgccgaca gcgaagcggt cagcacaaca
    27301 aagttaagaa tgctggcggc aaaggtaatg cccatatgct ggaacgtcag tacgaacggg
    27361 ctaccggcag tgccaacctg attccacggg tagatagaca taatgacgaa cagcgtaccg
    27421 acgtagaaca ccagaatacg catcggcacg gagttaatcg cacgcggaat cgatttctca
    27481 ggatctttcg cttcaccggc ggtaatcccg ataatttcga tcccaccgta agcaaacatc
    27541 accatttgca acgacattac catgccaagc cagccgttac tgaagaagcc gccgttgctc
    27601 cacaggttat gaataccggt cggttgcccg ccgttgccaa tcccccagat gatgatgccg
    27661 aaaccggcga caatcatgat gatgatggtg gcgactttaa agaacgagaa ccagaattcc
    27721 agctcaccga agaccttcac gctcatcagg tttacggcgc aaatgatcag caccacgctc
    27781 agtacccaaa tccagtgcgg caccgtcggg aacaagacac ccatatagat accaaaagcg
    27841 gtcacatcgg cgatggcgac aataaggatt tcaaaacagt aggtccatcc ggtaatgtaa
    27901 cctgccagcg ggccaaggtt ttcctgcgca taacgcgaga aagagctggc ggccgggtta
    27961 tgtaccgaca tttcccccag cgcacgcata atgatatacg ccgcgatacc accgataata
    28021 taggccaaca acacgctcgg accggccatt ttgatggcgt ctgccgaacc gtaaaacagc
    28081 ccggtgccaa ttgctgaacc cagtgccata aagcgtatgt ggcgggtact tagcccacgc
    28141 tttagcttat tcttactttc catcaattcc taacccatca acacaataaa aaaccacggg
    28201 gcatcgcagc cccgtggtta aaacaaatgt tcagtgattt agtgagcgct ggaggtcacc
    28261 tgacgacctg ccgcgcgatc ccagataatg gccagaacca ccatcaccac tgttggcatt
    28321 aaccacgcca gaccttgttc ggccagcggt aaacgctgcg cccaggacgg taagatatcg
    28381 ctgaatgcag atgctttgat cccgtcgaga ataccaaaaa gcaggctgat aaacatcggc
    28441 ggagcaatca cgcgggacga attatgccac catgagcgtg taaaacttaa tacaaccagt
    28501 gcgatacacg gcggataaat agcggtcagc accggtacgg agatctggat cagctggctt
    28561 aagccgaggt tagacaccac catcgagaag ccgccgagga taaacaccag cgtacgataa
    28621 gagagcggta cgtactgggc aaagaattct gcacaagcac aggtcaggcc aaccgccgtg
    28681 accagacagg cgatgaagat taacgccgcc agcaggaagc taccgccgcc gccaaaggtg
    28741 tgctgaacgt aagcatgcag aatagcagcg ccgtttgcag actgatcgac cagcgacgcg
    28801 ctgtctgaac ccagacagaa cagcgccagg tacagcaaag tcagaccaac acccgccatc
    28861 aggccagccc agacggtata acgggtcagc agacgcgctt cggtaacgcc acgagaacgc
    28921 gccgcgttaa caataacgat accaaacacc attgcgccca gcgtatccat ggtcagatag
    28981 ccgttaacga agccgttaga aaatgcagcg ttttgataag cctcagtcgc cgtgctgata
    29041 gaacccgccg gccagacaat cgcgacaaca gacaggatga ccagcgcgat aattttcagc
    29101 ggcgcaagga agttgcccac ggtatccagc agcttgcccg gatagagcga aaccagaata
    29161 acgatagcga aatagaccag gctgtagata aacagcggca gcgcggaatc acccgtcagc
    29221 ggcgcaatcc ccacttcaaa ggaaacggta gctgtacgcg gcgtagcgaa aagcggcccc
    29281 accgccaggt aacaaaccgt tgccagcagt acgccagcga ctttaccgat tggcgtgctg
    29341 aggctgtcaa caccgccgcc aacttttgcc agcgccacta ccgttaatac cggcaggcca
    29401 acggcagtaa tgaggaagcc gaatgccgca gtccagacgt gttcgcctgc ctgtaagccg
    29461 accattggag ggaaaataat gttacctgcg ccgacgaaca acgcaaatgt cataaagccc
    29521 agagcgatga tatcgcgcga tcttaattga tgggtcataa atcatactgc ctgtggatgt
    29581 ggtgttgtaa cgttaaattt ttgtgccaat ccctggggat gatacagcgg tatatttaaa
    29641 caattttagc gggaaatact cccaaccagg cgcggcgaag aatagttttt cgatttaccg
    29701 cgttaatgca gtcagtatga cagcacctga agcgcaattt aaacgcttat aacgttttaa
    29761 agcaaggagg gatctaaaaa ccagaggtat atagcgataa gaaatcacca accagtataa
    29821 aacactattt ccgtgaaaaa gacatggcta atcatgcgaa caaaaaatca ccaaccgttg
    29881 taaaatctaa cgcccgtatt ttgactgcaa agaaaataag ccagcatcgg ctggcttatg
    29941 gcaataaaag atgacaaagg cggattaatc gctgtttttg gcaattaaac gttccgggat
    30001 aacaaaactg aaacgtgttc cttttcctac tgtactctca atattcaggc gactttcgtg
    30061 atgattcacc gcatgtttca cgatcgctaa ccctaatccg ctaccgccgg tttgccggga
    30121 acgcgcttta tcaacgcgat aaaaacgctc ggtcaggcgc ggaatatgct ccggtgcaat
    30181 gcccggtccg ttatcttcaa cgctaaattc ggcaccgtgc ggcactcgct gccagcgtac
    30241 ggtgatatgc gtgccttccg gcgtatgatt cacggcgtta tagaccaggt tcgaaatcgc
    30301 actgcgtagc tgatcttcgt tgccagacac cttgaggccg ttatctatct caaaggtaaa
    30361 tgtctgtttt ttctgactca gagtctgagc ctcgcgctca acaacgcgca gcatcatcgg
    30421 cacatcaacc ttttcattga gcaattgcgt cggcgcggct tcaattttcg acagcgttag
    30481 caactgcttc accagccctt ccatccgctg ggtctgctcg cgcatggtgt gcaacgcttt
    30541 ttcgcgtacc gcgccttcca gcggctgctc atccatcatc tccaggtaac cctgtaacac
    30601 ggtcaatggc gtacgtaact catggctcac gttggcaaaa aagttacgcc gcgccccttc
    30661 cagttgatgc atttgcgtga catcacgcgc caccatcagc aactgtttgt gggtataagg
    30721 catgacgcga atttccagat gccgcccggt gttgagcacc agattgagcg ggcgagaaaa
    30781 atcacgcgtt ttcagatatt gcgtaaactc cgggtaacgc agtaggttaa ggatgttctg
    30841 cccgttatct tccggccagc gcaaaccaag aatttgttgc gccagaccgt tacaccagaa
    30901 aataccgccc tcttccgtgg tcagcaccac cgcgtcgggc agcgactccg cgccgctacg
    30961 aaagcgttta atcagattgc ccagttcacg gcggcgtttt ttatttcgca gctgcatctg
    31021 gtgtaagccg tatagtagcg gttcccagct accacgcccc ggtggcgggg tcatactgcg
    31081 atccacccac agccaccatg aaaggcgcaa taaattccag aaatgccaga taagcagtcc
    31141 tgttaccgat gccagcaaaa accagggcag gtaaccaaaa aatgcaccca ggatgaaagc
    31201 cgggaggcag caaagtagca gctccagcac cagccttttc cacgacagcc gttccagcac
    31261 gtaagatact ccagttaaga aatcataagc cctgctctgc gtccgatgag caaggcgtta
    31321 aaagcgggtt gaaaaacgat atcctgtacc gcgcacggtc tgcaccatgc ggtcatgccc
    31381 gccgggctcc agtgctttac gcaggcgacg aatgtggaca tcgaccgtgc ggtcttccac
    31441 atacacgtta gttccccaga cgtggtttaa cagctgctcg cggctgtaca cgcgctcagg
    31501 atgcgtcata aaaaagtgca gcagtttaaa ttctgtcggc cccatctcca gcggctcttc
    31561 gcccgccatc actcggtgag atgtcgggtc gagacttaat ccctgcatct taatcacctc
    31621 ttccaccgcc attggcgaaa tacggcgcat taccgctttg attcgcgcca ccagctcctt
    31681 cggcgaaaat ggcttggtga tatagtcatc cgcgccggtt tcaaggccgc gcacgcgatc
    31741 ttcttcttcc cctctggcgg tcaacatcac cactggaata tcccgggtca tcgactcgcg
    31801 cttgaggtgt ttgatgaact ggataccgga gccgccaggt aacatccagt caaggagaat
    31861 taaatccggc tagggttcat tcagttgatt cacagcactg tcataatctt ccgcttcgac
    31921 cggctgaaag ccattttgtt cgagcacgaa gcagaccatt tcgcgaattg gagcttcatc
    31981 ttctacgacc agaatacgtc tcgccatgat ttgccctgtt gtaataaata ggttgcgatc
    32041 attaatgcga cgtcattatg cgtcagattt atgacagatt tatgaaaagc tcgtcgcaca
    32101 tatcttcagg ttattgattt ccgtggcgca gaaaaaagca aatggcacat ctgtttgggt
    32161 ataatcgcga ccatgctttt tcgccaggga accgttatgc gcatccttca cacctcagac
    32221 tggcatctcg gccagaactt ctacagtaaa agccgcgaag ctgaacatca ggcttttctt
    32281 gactggctgc tggagacagc acaaacccat cagatggatg cgattattgt tgctggtgat
    32341 gttttcgata ccggctcgcc gcccagttac gcccgcacgt tatacaaccg ttttgttgtc
    32401 aatttacagc aaactggctg tcatctggtg gtactggcag gaaaccatga ctcggtcgcc
    32461 acgctgaatg aatcgcgcga tatcatggcg ttcctcaata caaccgtggt cgccagcgcc
    32521 ggacatgcgc cgcaaatctt gcctcgtcgc gacgggacgc caggcgcagt gctgtgcccc
    32581 attccgtttt tacgcccgcg tgacattatt tccagccagg cggggcttaa cggtattgaa
    32641 aaacagcagc atttactggc agcgattacc gattattacc aacaacacta tgccgatgcc
    32701 tgcaaactgc gcggcgatca gcctctgccc atcatcgcca cgggacattt aacgaccgtg
    32761 ggttccagta aaagtgacgc cgtgcgtgac atttatattg gcacgctgga cgcgtttccg
    32821 gcacaaaact ttccaccagc cgactacatc gcgctcgggc atattcaccg cgcacagatt
    32881 attggcggca tggaacatgt tcgctattgc ggttccccca ttccactgag ttttgatgaa
    32941 tgcggtaaga gtaaatatgt ccatctggtg acattttcaa acggcaaatt agagagcgtg
    33001 gaaaacctga acgtaccggt aactcaaccc atggcggtgc tgaaaggcga tctggcgtcg
    33061 attaccgcac agctggaaca gtggcgcgat gtatcgcagg agccacctgt ctggctggat
    33121 atcgaaatca ctactgatga gtatctgcat gatattcagc gcaaaatcca ggcattaacc
    33181 gaatcattgc ctgtcgaagt attgctggta cgtcggagtc gtgaacagcg cgagcgtgtg
    33241 ttagccagcc aacagcgtga aaccctcagc gaactcagcg tcgaagaggt gttcaatcgc
    33301 cgtctggcac tggaagaact ggatgaatcg cagcagcaac gtctgcagca tcttttcacc
    33361 acgacgttgc gtaccctcgc cggagaacac gaagcatgaa aattctcagc ctgcgcctga
    33421 aaaacctgaa ctcattaaaa ggcgaatgga agattgattt cacccgcgag ccgttcgcca
    33481 gcaacgggct gtttgccatt accggcccaa ccggtgcggg gaaaaccacc ctgctggacg
    33541 ccatttgtct ggcgctgtat cacgaaactc cgcgtctctc taacgtttca caatcgcaaa
    33601 atgatctcat gacccgcgat accgctgaat gtctggcgga ggtggagttt gaagtgaaag
    33661 gtgaagcgta tcgcgcgttc tggagccaga atcgggcgcg taaccagccg gacggtaatt
    33721 tgcaggtgcc tcgcgtagag ctggcgcgct gcgccgacgg caaaattctc gccgacaaag
    33781 tgaaagataa gctggaactg acagcgacgt taaccgggct ggattacggg cgcttcaccc
    33841 gttcgatgct gctttcgcag gggcaatttg ctgccttcct gaatgccaaa cccaaagaac
    33901 gcgcggaatt gctcgaggag ttaaccggca ctgaaatcta cgggcaaatc tcggcgatgg
    33961 tttttgagca gcacaaatcg gcccgcactg agctggagaa gctgcaagcg caggccagcg
    34021 gcgtcacgtt gctcacgccg gaacaagtgc aatcgctgac agcgagtttg caggtactta
    34081 ctgacgaaga aaaacagtta attaccgcgc agcagcaaga acaacaatcg ctaaactggt
    34141 taacgcgtca ggacgaattg cagcaagaag ccagccgccg tcagcaggcc ttgcaacagg
    34201 cgttagccga agaagaaaaa gcgcaacctc aactggcggc gcttagtctg gcacaaccgg
    34261 cacgaaatct tcgtccacac tgggaacgca tcgcagaaca cagcgcggcg ctggcgcata
    34321 ttcgccagca gattgaagaa gtaaatactc gcttacagag cacaatggcg cttcgcgcga
    34381 gcattcgcca ccacgcggcg aagcagtcag cagaattaca gcagcagcaa caaagcctga
    34441 atacctggtt acaggaacac gaccgcttcc gtcagtggaa caacgaactg gcgggttggc
    34501 gtgcgcagtt ctcccaacaa accagcgatc gcgagcatct gcggcaatgg cagcaacagt
    34561 taacccatgc tgagcaaaaa cttaatgcgc ttgcggcgat cacgttgacg ttaaccgccg
    34621 atgaagttgc taccgccctg gcgcaacatg ccgagcaacg cccactgcgt cagcgcctgg
    34681 tcgcgctgca tgggcagatt gttccccaac aaaaacgtct ggcgcagtta atcgtcacta
    34741 tccagaatgt cactctggaa cagacgcaac gtaatgtcgc acttaacgaa atgcgccagc
    34801 gttataaaga aaagacgcag caacttgccg atgtgaaaac catttgcgag caggaagcgc
    34861 gcatcaaaac gctggaagcc cagcgtgcac agttacaggc gggtcagcct tgtccacttt
    34921 gtggttccac cagccacccg gcggtcgagg cgtatcaggc gctggagcct ggtgttaatc
    34981 agtctcgatt actggcgctg gaaaacgaag ttaaaaagct cggcgaagaa ggtgcggcgc
    35041 tgcgcgggca actggatgca ttaacgaagc agcttcagcg cgatgaaaac gaagcgcaaa
    35101 gcctccgaca agatgagcaa gcacttactc aacaatggca agccgtcacg gccagcctca
    35161 atatcacctt gcagccacag gacgatattc aaccgtggct ggatgcacaa gatgagcacg
    35221 aacgccagct gcggttactc agccaacgac atgaattaca agggcagatt gccgcgcata
    35281 atcagcaaat tatccagtat caacagcaaa ttgaacaacg tcagcagcag ctcttaacgg
    35341 cattggcggg ttatgcactg acattgccac aggaagatga agaagagagc tggttggcga
    35401 cacgtcagca agaagcgcag agctggcagc aacgccagaa cgaattaacc gcgctgcaaa
    35461 accgtattca gcagctgaca ccgattctgg aaacgttgcc gcaaagtgat gatctcccgc
    35521 acagcgaaga aactgtggcg ctggataact ggcggcaggt tcatgaacaa tgtctcgcat
    35581 tacacagcca gcagcagacg ttacagcaac aggatgttct ggcggcgcaa agtctgcaaa
    35641 aagcccaagc gcagtttgac accgcgctac aggccagcgt ctttgacgat cagcaggcgt
    35701 tccttgcggc gctaatggat gaacaaacac taacgcagct ggaacagctc aagcagaatc
    35761 tggaaaacca gcgccgtcag gcgcaaacgc tggtcactca gacagcagaa acgctggcac
    35821 agcatcaaca acaccgacct gacgggttgg ctctcactgt gacggtggag cagattcagc
    35881 aagagttagc gcaaactcac caaaagttgc gtgaaaacac cacgagtcaa ggcgagattc
    35941 gccagcagct gaagcaggat gcagataacc gtcagcaaca acaaacctta atgcagcaaa
    36001 ttgctcaaat gacgcagcag gttgaggact ggggatatct gaattcgcta ataggttcca
    36061 aagagggcga taaattccgc aagtttgccc aggggctgac gctggataat ttagtccatc
    36121 tcgctaatca gcaacttacc cggctgcacg ggcgctatct gttacagcgc aaagccagcg
    36181 aggcgctgga agtcgaggtt gttgatacct ggcaggcaga tgcggtacgc gatacccgta
    36241 ccctttccgg cggcgaaagt ttcctcgtta gtctggcgct ggcgctggcg ctttcggatc
    36301 tggtcagcca taaaacacgt attgactcgc tgttccttga tgaaggtttt ggcacgctgg
    36361 atagcgaaac gctggatacc gcccttgatg cgctggatgc cctgaacgcc agtggcaaaa
    36421 ccatcggtgt gattagccac gtagaagcga tgaaagagcg tattccggtg cagatcaaag
    36481 tgaaaaagat caacggcctg ggctacagca aactggaaag tacgtttgca gtgaaataac
    36541 tattcagcag gataatgaat acagaggggt gaattatctc ttggccttgc tggtcgttat
    36601 cctgcaagct atcactttat tggctacggt gattggtagc cgttctggtg gttgtgatgg
    36661 tggtatgaaa aaagtcattt tatctttggc tctgggcacg tttggtttgg ggatggccga
    36721 atttggcatt atgagcgtgc tcacggagct ggcgcataac gtaggaattt cgattcctgc
    36781 tgccgggcat atgatctcgt attatgcact ggtggtggtg gtcggtgcgc caatcatcgc
    36841 actcttttcc agccgctact cactcaaaca tatcttgttg tttctggtgg cgttgtgcgt
    36901 cattggcaac gccatgttca cgctctcttc gtcttacctg atgctcgcca ttggtcggct
    36961 ggtatccggc tttccgcatg gcgcattttt tggcgtcgga gcgatcgtgt tatcaaaaat
    37021 tatcaaaccc ggaaaagtca ccgccgccgt ggcggggatg gtttccggga tgacagtcgc
    37081 caatttgctg ggcattccgc tgggaacgta tttaagtcag gaatgctggc gttacacctt
    37141 tttattgatc gctgttttta atattgcggt gatggcatcg gtctattttt gggtgccaga
    37201 tattcgcgac gaggcgaaag gaaatctgcg cgaacaattt cactttttgc gcagcccggc
    37261 cccgtggtta attttcgccg ccacgatgtt tggcaacgca ggtgtgtttg cctggttcag
    37321 ctacgtaaag ccatacatga tgtttatttc cggtttttcg gaaacggcga tgacctttat
    37381 tatgatgtta gttgggctag ggatggtgct gggaaatatg ctaagtggca ggatttcagg
    37441 acgttattca ccactgcgca ttgcagcagt gactgacttt atcattgtac tggcactgct
    37501 gatgctcttt ttctgcggcg gcatgaaaac aacgtcgctt atttttgctt ttatttgttg
    37561 cgcgggatta tttgcccttt cagcacctct gcaaatattg ttactgcaaa atgccaaagg
    37621 aggagagtta ttaggtggcg caggtgggca aatagcgttt aacctcggta gcgccgtcgg
    37681 cgcatattgc ggaggtatga tgctgacgct ggggctggca tataattacg tggcgctgcc
    37741 tgccgccctg ctttcgtttg ctgcgatgtc atcgttgctg ctgtatggtc gctataagcg
    37801 ccagcaagcg gcggatagtc cggtgctggc gaaaccactg gggtagatta ctgtcacggt
    37861 gcgcgttgct tattgccgga tgcggcgtga acgccttatc cgccctacgc ggttctggca
    37921 cattttgtag gcctgataag acgcggcaag cgtcgcatca ggcatgatgc gccaattgcc
    37981 tacgtttttt actcttgtgg ccataaccac gcagcgccgc gtacgccgct ggaatcaccg
    38041 tgcttcgcct tactcaccgg cgtttcacat tcgccgccga agacaaattg tttaatcaac
    38101 tgcccaaccg tttgatataa acggtctaca ttgctcatcc cgccccccag gacaatcaca
    38161 tccggatcga gaatattcac gacatgtgcc agcgattttg ccagccgcag ctcgtagcga
    38221 cgcaatgcca gttccgctac cggatcgctt tcttcaacca ggcggataat ttcactgcct
    38281 ttcagcgcat gtccgctcaa acgacgataa tccgtcgcga atcccgtgcc cgaaataaag
    38341 gtttcaatac aaccttgttt accgcaataa caagggactt cctcgcgata acgcagttcg
    38401 tcttcgtcca tccacggtag cggattgtgt ccccactcac ctgccgtgcc attgccgccg
    38461 atatgcgtcc gcccattgaa tgccacgccc gcgccgcatc ccgtgccgat agtcacggca
    38521 aataccgtct gcgctcccgc tgccgcgcca tctactgctt ctgaaaccgc cagacagttg
    38581 gcatcatttg ccagccgcac ttcccgctgc aacctcgcgc ttaagtcttt atcgaatggc
    38641 tgaccgttga gccaggttga attgacattc ttcaccacac cggtgtaggg cgaaatcgaa
    38701 cctggaatgc ccatacctac cgttccgcgc tgccctgtcg cctgctccgc catatcaacc
    38761 aacgtggcga tcgtttcaat agtctgccgg taatcatcac gcggcgtggg cagacgatgg
    38821 cggtacaact gctcccctgc atcgcccagt gcaatcactt cagttttggt gccgcctaaa
    38881 tcgataccta tacgcactgt actctcctta tttttttcaa tatcaatagc gtagagacgg
    38941 acaaccggat tggcaatgca aggccgccga caattcgtta tcatgcccgc taaatttaac
    39001 gacaaggccg tggaaattat catgctgtgg ttcaaaaatt taatggttta ccgtcttagc
    39061 cgcgagattt cgctgcgtgc agaagagatg gaaaaacagc tagcctcgat ggcatttacc
    39121 ccatgcggca gccaggacat ggcgaagatg ggctgggttc ctccgatggg atcgcacagc
    39181 gatgcgttaa cgcacgttgc caatggtcaa attgttatct gcgcgcgcaa agaagaaaaa
    39241 atcctcccgt ctccggtgat taaacaggcg ctggaagcga aaatcgccaa actggaagcg
    39301 gaacaggcgc gtaagctgaa gaaaaccgaa aaagattcgc tgaaagacga agtgctgcac
    39361 tctctgctgc cgcgtgcttt cagccgtttt agccagacaa tgatgtggct cgacacggtt
    39421 aacggtttga ttatggtgga ctgcgccagt gccaaaaaag cggaagatac gctggcatta
    39481 ctgcgtaaaa gcctggggtc gttaccggtt gtaccattga gcatggaaaa cccgattgaa
    39541 ctgacgctga ccgaatgggt tcgctccggt agtgcggcac agggcttcca gctgcttgat
    39601 gaagccgagc tgaaatcgtt gctggaagat ggcggcgtga tccgcgcgaa gaaacaagat
    39661 ctgaccagcg aagagatcac caatcacatt gaagccggaa aagtggtgac taaactggcg
    39721 ctcgactggc agcagcgcat tcagtttgtg atgtgcgacg atggttcgct caagcgtctt
    39781 aagttctgcg acgagctgcg cgatcaaaac gaagatatcg accgtgaaga tttcgcccag
    39841 cgttttgatg ccgatttcat cctgatgact ggtgaactgg cagcgttaat tcaaaacctg
    39901 attgaaggat taggtggcga agcacaacgt taattgctga ttttccttta atgccggatg
    39961 cgacgcctgc cgcgtcttat ccggcgtacg aagccacacc aagcatataa ttattcgcta
    40021 cggcgagcaa taatttttag cgcagcaata ttatgcgttt tacgccgtaa ctgatctaat
    40081 cgcctcatcc ctattcgcct gtcagcagca cgcagaatat tcactaaccc ccatttatcg
    40141 ctggtaaatt gttttgttaa cagctccgct tttggggtat caatatattc acgcaataaa
    40201 tcagaaattg aacaaatatc cgtcaggtaa ggataccagc aagtttcacc gtgaaaatta
    40261 cgcactccag cgttacctgc aataaagtct ttcgggtaat cccagatcac gtttttatcc
    40321 aacgtgatcc aataacgcgg taaatcggta cttccaccat tttggctgcg catgggataa
    40381 cgggtacagt gaatttgcag gttgatcgtt ggcgtcagta agtcatagat ctcccgctgt
    40441 aacttgctcc atggacgttg tgtcattgtt tttcctcaag ccggtgacgc gcttcactgc
    40501 gaattttccg cgttgccccg gttttcacta aatcacgtaa ctgcgaaaga tcgggttatt
    40561 cgacaaataa gagtaatgat ggatcctctt ttaaccacgc cgttttcaca tcggcagcca
    40621 gctgtggatt attaatagcc agcgaccgat cctgaggctc agtcaatgtc gttaacaacg
    40681 acgccggggt atgtgcgttg cgtaatgccg tttggcgcaa atgatcattg cgactttgca
    40741 gcaatgcacg ccaccattca tcatctgatt tatcttttat cgtagccagt tccgcaaaaa
    40801 atacctggag atcttcactg atatttatat caaccttgat gaattatgaa tatatcggaa
    40861 attgaaaaaa tcgcagcaaa ggaaatatta ttatgggtta aagagaggat atttcagaaa
    40921 aatgccctcc cgttcaaggg gaaggcgagg aattacagat agcggcacag ataagaggta
    40981 ggttcggcaa cttgaagatg aaactcactg tgaccgggaa cattaaacac cgaaccggct
    41041 tcatacactt gccagtcggt cgcgtcaggc agtaacacat tcagcgcgcc actgattacc
    41101 gtcatctctt ccggctcagc ggtgctgaag gtgtattcgc cttcaaccat aacacccacg
    41161 ctggcgcgac cagtgctgct gctggaaaag ccgatcgatt tcactttgcc ggaaaagtac
    41221 tcattacttt gaagcataaa ctggccctgt ctggtagttg aacatctact tcactatagg
    41281 ggccagaggc gctggctgtc acgcaaaatt acaccagtaa ttccgcagcc agccgtgcaa
    41341 tcaatacgtt agagagcaag acaggaacat cgagctgttt ttgcagtaaa tcgcgatgac
    41401 gttggtgaaa tcctaaacaa tccagcatga tgacatcagc cccttttgcc agtaattctt
    41461 tcccagcatc aatgattttt tgttctgaat catgaatggg gttacccaat gaaaataccg
    41521 gcgatttctg caaaatttgc catttttgcg cctgaacggg cagcatctcc tcaaccggaa
    41581 cgataacccc cacctgatga tcttcaacaa tagaggaaac cagtggaggc aatattcgcg
    41641 acggctcaag aaagatcgta ttacgcgcag tcatactact aatgtttgct gtactcatta
    41701 ataaaatgac gtcataaccc tgattatcga gcacttcaac cacaccttgc agatctcgct
    41761 ccactttacg acgcgaaaca tgggccagct ggttgtcatt taataatgtg agaatggtgt
    41821 cttcgcctgc ttctggcgcg tactccgcca tcacttcttc acgacttaac ttccccagca
    41881 ggctatgatg ggaaatatta tcttcgtcaa tgtattccgt caggagcggc aaaacctcct
    41941 gcataggtac aatgccgatg gtcaggatcg ccaacgacgc actcattgtt catccacctt
    42001 acttttcttt cgtcgttaat taccgggcaa gtgtgaagca ccactgctga cacttacttg
    42061 tgctacaaat gacaaaaagc gtagcagcag acgtgcacgg cataatgtaa agattccaaa
    42121 tgattccagt aatggaatgt tattgtttaa tattctaatt attagaaaaa cataaattat
    42181 gaaaaatgtg acgcagatcc cttgtagtgg cactcagaat cccttccgag cagtctgatg
    42241 cggtcactat ttgtcagggt cctcatagcg ttttttcgca tccatcgctt cctgagccgt
    42301 cggatgttca ctgatcaacg agtctggttt aggatgatcg gctctgagtt ggtaccaggt
    42361 taccgtctgt cctggctttc ctttttcaat cgtcactata tatgcttcac gaggataagg
    42421 cggtttcgtt ggcatcgttc ttccttattt cacgggatga acgttaagta taggcgctct
    42481 aaaatcaaca attgatcgtc tgtgccaggg cgctacgaat ttcagaaatc acctggctgg
    42541 gttcgtttgt tgcgtcgatg ataatatgcg caacttcgcg atatagcgca tcgcgttctt
    42601 ccagcacttc ctgaacttct tcgctcagcg gttttcccgt taaggttggc cgtaaatctt
    42661 cttccggtgc agcttgcagt cggttaacca ggactgatac tggcgcacac aaataaacca
    42721 cgatcccgtt attttgcatg aagtgacgat taaattccgt cagaataatg ccgccgcctg
    42781 tagcgataac ggtggatgcc gcagttaccg cttccagcgc cgccgtttct ctggcgcgaa
    42841 atcccgccca ctcttccctt tcgacgatct ccgcgaccgt catattgagc tgtgattgca
    42901 accactgatc ggtatcgaca aaccgacggt taagcgaatc ggcaagggcc attccgaccg
    42961 ttgttttacc acagccccga ggcccgatca gaaaaagagg ttgtgtcatc gtgggttttc
    43021 cccaataggt cgcaatgcgg cgaaagccgg tgtcatgaga atagcgatca taccatcaaa
    43081 ctagtacaat ttcgattgta aagaaaaaat tccacttaaa gtaaaaatct caatacatcc
    43141 cttactatac caataaatat tcaagaatga agtgtaaata ataaattaca tttagccacg
    43201 actacgttgc acttccagcc accacttctc cagctccgcg gcaaaggcct ggcggtcacg
    43261 ttgtgaaagg ctatctggtc cgccggtctg gatcccactg gcacgtaagg tatccataaa
    43321 atcgcgcatc gtcaggcgct cacgaatggt cgctggcgtg taacgttcgc cgcgcgaatt
    43381 aagcgccgct gcgcctttct cgatggcttc agcagccaaa ggtatatctg cggtgatcac
    43441 caaatcgccc gcttcacact gccggacaat ttcgttatcg gcaacgtcga aacctgccgc
    43501 gacgcgcagc gtacgaataa atcgcgatgg cggcacgcgt aaactctggt ttgctaccag
    43561 taccagcggc atctgcatac gttccgccgc gagatacaaa atctctttaa ttacattggg
    43621 acacgcgtcg gcatccaccc aaattgtcat aaagtcatcc tttgttaggt aatcctctat
    43681 tgtgtcgcgc ttttgccttc cggcatagtt ctgtttatgc ttctgccagc gattatcaaa
    43741 acaatgaatt tcacggcagg agtgaggcaa tggaaaagaa aatcggtttt attggctgcg
    43801 gcaatatggg aaaagccatt ctcggcggtc tgattgccag cggtcaggtg cttccagggc
    43861 aaatctgggt atacaccccc tccccggata aagtcgccgc cctgcatgac cagttcggca
    43921 tcaacgccgc agaatcggcg caagaagtgg cgcaaatcgc cgacatcatt tttgctgccg
    43981 ttaaacctgg catcatgatt aaagtgctta gcgaaatcac ctccagcctg aataaagact
    44041 ctctggtcgt ttctattgct gcaggtgtca cgctcgacca gcttgcccgc gcgctgggcc
    44101 atgaccggaa aattatccgc gccatgccga acactcccgc gctggttaat gccgggatga
    44161 cctccgtaac gccaaacgcg ctggtaaccc cagaagatac cgctgatgtg ctgaatattt
    44221 tccgctgctt tggcgaagcg gaagtaattg ctgagccgat gatccacccg gtggtcggtg
    44281 tgagcggttc ttcgccagcc tacgtattta tgtttatcga agcgatggcc gacgccgccg
    44341 tgctgggcgg gatgccacgc gcccaggcgt ataaatttgc cgctcaggcg gtaatgggtt
    44401 ccgcaaaaat ggtgctggaa acgggagaac atccgggggc actgaaagat atggtctgct
    44461 caccgggagg caccaccatt gaagcggtac gcgtactgga agagaaaggc ttccgtgccg
    44521 cagtgatcga agcgatgacg aagtgtatgg aaaaatcaga aaaactcagc aaatcctgat
    44581 gactttcgcc ggacgtcagg ccgccacttc ggtgcggtta cgtccggctt tctttgcttt
    44641 gtaaagcgcc aaatctgccg atttcaacca ctcacgatag tgactcattt gtgggttcag
    44701 cggcgcaacc cccacactaa tccgtaaagt tacctgtggc gtattcggca agcgtaacgt
    44761 gtttagccct tcacgcaccc gtaacatggc ggtaatggcg ctctcagctg gcgtaccgga
    44821 catgattact gcaaactcat cgccgccaaa ccgaccaatc acatcgctac cgcgcagggt
    44881 aatttgtaac tgtcgggtaa gcgccacaat cgcttcatcg cccacatcat gaccccaggt
    44941 atcgttgatg ctcttgaaat ggtcgatatc gataatcagt aacgttgcat cgcgattatg
    45001 ccgccgacag ttatcaaatt cattgcgtaa catagtttcc caatgacgtc ggttatacac
    45061 gcccgtcatg ccgtcgcgag tactcatgac ctgcaacctg cgtttatgtt ccgccagttt
    45121 ggttgccgtc tggtagctga cccagccaaa catcagagga taaacgacaa taatgggaag
    45181 ggagagccac cattccagcg gagcactatt gaacgacacg gtaatgcccg taagctcgag
    45241 ggtgacaagg caggaaacca ccatcaacac cagacccgcg acaaacagac gggggccgcc
    45301 tgcccccatc aaattcagac acagaatcat caacatagcg gtggaaggca gcacgtttac
    45361 gcccattacg cctacccaca ttcccgctaa tactgcatcg gtttttaagt tgtaaatttc
    45421 ccggctaagc ggatcgacgg ccctgctcgc tatctgccag gctaaatgcg gccagacgaa
    45481 cgcccagccg accaacacca gccaccacca gcccggcggc gggtgcgaaa ccagcgttga
    45541 agcaatcggt aagaacatgc cagccaggcc aaccgcacgg ggtagtctga cgcgacgggc
    45601 gaagcgcagc ccggaccgct gatgttcgtt ttgaggagtt aaaggaggtt cctccccatg
    45661 cgccgccgct tttttg
//
LOCUS       DAJTQY010000025        40804 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.025, whole genome shotgun sequence.
ACCESSION   DAJTQY010000025 DAJTQY010000000
VERSION     DAJTQY010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40804
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.025"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(181..858)
                     /gene="narI"
                     /locus_tag="OO349_001419"
     CDS             complement(181..858)
                     /gene="narI"
                     /locus_tag="OO349_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HCR5866551.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRMFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(858..1568)
                     /gene="narJ"
                     /locus_tag="OO349_001420"
     CDS             complement(858..1568)
                     /gene="narJ"
                     /locus_tag="OO349_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HCR5866552.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQVQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(1565..3103)
                     /gene="narH"
                     /locus_tag="OO349_001421"
     CDS             complement(1565..3103)
                     /gene="narH"
                     /locus_tag="OO349_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCR5866553.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(3100..6843)
                     /locus_tag="OO349_001422"
     CDS             complement(3100..6843)
                     /locus_tag="OO349_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HCR5866554.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGYLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(7236..8627)
                     /gene="narK"
                     /locus_tag="OO349_001423"
     CDS             complement(7236..8627)
                     /gene="narK"
                     /locus_tag="OO349_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HCR5866555.1"
                     /translation="MSHSSASERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATGTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            8966..10762
                     /gene="narX"
                     /locus_tag="OO349_001424"
     CDS             8966..10762
                     /gene="narX"
                     /locus_tag="OO349_001424"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HCR5866556.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            10755..11405
                     /gene="narL"
                     /locus_tag="OO349_001425"
     CDS             10755..11405
                     /gene="narL"
                     /locus_tag="OO349_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HCR5866557.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(11406..12800)
                     /gene="ychO"
                     /locus_tag="OO349_001426"
     CDS             complement(11406..12800)
                     /gene="ychO"
                     /locus_tag="OO349_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HCR5866558.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDNGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLQDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSLEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            12985..13338
                     /gene="ychN"
                     /locus_tag="OO349_001427"
     CDS             12985..13338
                     /gene="ychN"
                     /locus_tag="OO349_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HCR5866559.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(13382..14077)
                     /gene="chaC"
                     /locus_tag="OO349_001428"
     CDS             complement(13382..14077)
                     /gene="chaC"
                     /locus_tag="OO349_001428"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HCR5866560.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(14235..14465)
                     /gene="chaB"
                     /locus_tag="OO349_001429"
     CDS             complement(14235..14465)
                     /gene="chaB"
                     /locus_tag="OO349_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HCR5866561.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            14735..15835
                     /gene="chaA"
                     /locus_tag="OO349_001430"
     CDS             14735..15835
                     /gene="chaA"
                     /locus_tag="OO349_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HCR5866562.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLVLVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQAKTHQSLFVYEHEDDSDDDDPHHGKTSAH
                     SSLWHAIWLIIHLIAVIAVTKMNASSLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            16240..16347
                     /locus_tag="OO349_001431"
     CDS             16240..16347
                     /locus_tag="OO349_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCR5866563.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIIAAAIVSWWRNRK"
     gene            complement(16496..17350)
                     /gene="kdsA"
                     /locus_tag="OO349_001432"
     CDS             complement(16496..17350)
                     /gene="kdsA"
                     /locus_tag="OO349_001432"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HCR5866564.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(17386..18195)
                     /locus_tag="OO349_001433"
     CDS             complement(17386..18195)
                     /locus_tag="OO349_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HCR5866565.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDGWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            complement(18199..18591)
                     /locus_tag="OO349_001434"
     CDS             complement(18199..18591)
                     /locus_tag="OO349_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HCR5866566.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            complement(18588..19421)
                     /gene="prmC"
                     /locus_tag="OO349_001435"
     CDS             complement(18588..19421)
                     /gene="prmC"
                     /locus_tag="OO349_001435"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCR5866567.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            complement(19421..20503)
                     /gene="prfA"
                     /locus_tag="OO349_001436"
     CDS             complement(19421..20503)
                     /gene="prfA"
                     /locus_tag="OO349_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HCR5866568.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            complement(20545..21801)
                     /gene="hemA"
                     /locus_tag="OO349_001437"
     CDS             complement(20545..21801)
                     /gene="hemA"
                     /locus_tag="OO349_001437"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HCR5866569.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFVDSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            22015..22638
                     /gene="lolB"
                     /locus_tag="OO349_001438"
     CDS             22015..22638
                     /gene="lolB"
                     /locus_tag="OO349_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HCR5866570.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            22638..23489
                     /gene="ispE"
                     /locus_tag="OO349_001439"
     CDS             22638..23489
                     /gene="ispE"
                     /locus_tag="OO349_001439"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HCR5866571.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            23640..24587
                     /gene="prs"
                     /locus_tag="OO349_001440"
     CDS             23640..24587
                     /gene="prs"
                     /locus_tag="OO349_001440"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HCR5866572.1"
                     /translation="MPDMKLFAGNATPVLAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            24712..26391
                     /gene="dauA"
                     /locus_tag="OO349_001441"
     CDS             24712..26391
                     /gene="dauA"
                     /locus_tag="OO349_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HCR5866573.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(26446..26724)
                     /gene="ychH"
                     /locus_tag="OO349_001442"
     CDS             complement(26446..26724)
                     /gene="ychH"
                     /locus_tag="OO349_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HCR5866574.1"
                     /translation="MKRKNASILGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            27002..27586
                     /gene="pth"
                     /locus_tag="OO349_001443"
     CDS             27002..27586
                     /gene="pth"
                     /locus_tag="OO349_001443"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCR5866575.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            27703..28794
                     /gene="ychF"
                     /locus_tag="OO349_001444"
     CDS             27703..28794
                     /gene="ychF"
                     /locus_tag="OO349_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HCR5866576.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            29637..32504
                     /locus_tag="OO349_001445"
     CDS             29637..32504
                     /locus_tag="OO349_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR5866577.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKIKTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSTIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSSSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVINMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFTTLNVENLSGNSTFIMRADVVGEGNGVNNRGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPEPAPTPKPTTTADAGGNYLNVGYLLNYVENRTLMQRM
                     GDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMPLYVGLY
                     IDSTHASPDYSGGDGTARSDYMGMYASYMAQNGFYSDLVIKASRQKNSFHVLDSQNNG
                     VNANGTANGMSISLEAGQRFNLSPTGYGFYIEPQTQLTYSHQNEMAMKASNGLNIHLN
                     HYESLLGRASMILGYDITAGNSQLNVYVKTGAIREFSGDTEYLLNDSREKYSFKGNGW
                     NNGVGVSAQYNKQHTFYLEADYTQGNLFDQKQVNGGYRFSF"
     gene            complement(32604..34523)
                     /gene="dhaR"
                     /locus_tag="OO349_001446"
     CDS             complement(32604..34523)
                     /gene="dhaR"
                     /locus_tag="OO349_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase operon
                     transcriptional regulator DhaR"
                     /protein_id="HCR5866578.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTVDLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            34751..35821
                     /gene="dhaK"
                     /locus_tag="OO349_001447"
     CDS             34751..35821
                     /gene="dhaK"
                     /locus_tag="OO349_001447"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HCR5866579.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECSMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLEACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLEHGSYHRTLRFWDYQQGSWQEEPQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            35832..36464
                     /gene="dhaL"
                     /locus_tag="OO349_001448"
     CDS             35832..36464
                     /gene="dhaL"
                     /locus_tag="OO349_001448"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HCR5866580.1"
                     /translation="MSLSRTQIVNWLTRCGDIFNTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            36475..37893
                     /gene="dhaM"
                     /locus_tag="OO349_001449"
     CDS             36475..37893
                     /gene="dhaM"
                     /locus_tag="OO349_001449"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HCR5866581.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPPYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRHLAEDNFGETEEVAPPTLRPVLPVSGKAFYYQPVLCTVQAKSTL
                     TAEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLGDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
     gene            38213..39910
                     /gene="treA"
                     /locus_tag="OO349_001450"
     CDS             38213..39910
                     /gene="treA"
                     /locus_tag="OO349_001450"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HCR5866582.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEINTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATHPTVKSATTQPSTKEAQPTP"
     gene            complement(39989..40428)
                     /locus_tag="OO349_001451"
                     /pseudo
     CDS             complement(39989..40428)
                     /locus_tag="OO349_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT        10088 a        10575 c        10176 g         9965 t
ORIGIN      
        1 cttccttgaa taatcaaaac ctgttactcc gagctaattt tgcccgatgg caccacgttt
       61 accgggccta caatatcctc ccctgtaggt cggataagcg aagcgcatcc gacaactcac
      121 cccgcaccgt gcccataaaa aaaccccgcc gaagcagggt ctatgtgaac taaaattcgt
      181 ttagtgacga gcgcgcacca gctggtactt acgtgtcaga tactccaccg gtacgctcca
      241 gatgtgtacc agacgcgaga acgggaacag caggaataac gtcatcccca gcaccaggtg
      301 cagacggaag ataaacgcca caccatcgag gtgttgagaa gcgccaccgt ggaaggtcac
      361 caccgactgc gcccagccaa ccagtttcat catctcgcta ccgtccatat gctgagcgga
      421 gaacggaatg gtcagcaggc ccagcgcgca ctggataacg agcagcgaca ggatcaggat
      481 atccgctccg gtagtggttg cacgcacgcg tgggctgaac atacgacgtt tcagcaaaag
      541 cacgccgcca atcagacaca gcacgccgct ggcaccacca gcaaacatcg ccattttctg
      601 tttcacttca atcggcagcc aggcttcata catccagtgc ggcgtcaaca taccgaagaa
      661 gtgaccgaca aaaatcccca gaataccgat atggaacagg ttcgacgcca ggttcatccc
      721 tttgcgatcc agcatctggc tggacgccgc gcgccaggtg tactgcccgt agtcataacg
      781 cagccagcta ccaatcagga agaccgcccc ggctatgtac gggtagatat caaagaagaa
      841 catattcagg aattgcatta gtgctgtcct ccggtggtga tattcagata ttgcggcgcg
      901 acggcaccgg caaagcgacg ctgatgagca gtgattgctg aatcaccgca gcctttgtca
      961 gcaaagaatt taacctgctc ttcttcccag acagcatcca gcgcctgcgg cgtatcatcg
     1021 cgcgcttcgt cggcaatttt ttccgccact ttgtcgctgt cgatagcggt attggccaat
     1081 ttcagcagca gatcaaacat cacggcataa cggctttcac gctgttgcag acgcgcgctc
     1141 agcaatgcca gaatcggcgc gatatctttc aaaccttcca cggcttcgct ttgcggcagc
     1201 tgcgacaggt actccagata cagcggcaga tggtccggta attcgcggct gtttaactgc
     1261 aagccgtgct gctcgtactg cgccagcagg tccaccatcg cctggccgcg gtcacgggat
     1321 tcaccgtgaa catgttcaaa tagcaacagt gacgtggcgc ggccacggtc gaacagttcg
     1381 ctgtactgga cctgggcatc gagcggatcc atcgccgtta aatcgcgcag gaaaatgccc
     1441 agcgcatggg catcttcttt cgacagattt ttcgacgcag caatcgcctc aaacatctct
     1501 tgttgatgct gccataaggc agcatccgga tattcaagga gacgcgatac aatcacgagt
     1561 tcgatcatgg atgcggctcc gttttgctgg tcacatcgat ggcatcgata cgacggctgt
     1621 tgaacagatt gaatttggta tctgagccgt ggcagccatc accaaaggta aagccgcagc
     1681 catttttctc cgggaaggct tcccgtgcca gttcacgatg actactcggc accacaaagc
     1741 gatcttcgta gttagcaatc gccagataac ggtacatctc ctgtgcctgg gcttcggtca
     1801 gaccgacctc ttccagcgca cgggtatcaa ctttaccgtc aacagtttca gcacgtttgt
     1861 aatgacgcat cgccagcata cgtttcagcg cacgcagtac cggtttggta tcaccggcgg
     1921 tcagcagatt cgccagatac tgtaccggaa tacgcaagct ttcgacgtct ggcagaattc
     1981 cgttgctgcc cagctcgccc gcgtctgctg cagactgaat cggagacaga ggcggcacgt
     2041 accagaccat cggcagtgtg cgatattctg gatgcagcgg cagcgctagt ttccattcca
     2101 tcgccatttt ataaaccggc gactgctgtg cagcttcaat aacgctcagc ggaataccgt
     2161 ctttaatcgc ctgctcgatg actttcggat cgttcggatc gaggaacacg tccagctgac
     2221 gctggtagag atctttctcg ttctcggtgc tggctgcacg ttcaatcgcg tcggcatcgt
     2281 acagcagcac gccaagataa cggatacgac cgacacaggt ttctgagcac acggtcggct
     2341 gacccgcttc aatacgcgga tagcagaaga tgcacttctc agacttaccg ctcttccagt
     2401 tgaagtagat ttttttgtac gggcatccgg tgatgcacat acgccagcca cggcatttat
     2461 cctggtcgat caggacgatg ccatcttctt cacgcttgta aatcgcaccg ctcgggcagg
     2521 tcgccacaca tgccgggttc aggcagtgtt cgcacaggcg cggcaaatac atcataaagg
     2581 tgttttcgaa ctggctatac atcgccttct ggatgttgtc gaagttcttg tctttcgcca
     2641 gtttatcaaa ctcaccaccc aggtcatctt cccagttcgg ccctttttcg attttcgcca
     2701 tccgttcccc ggtaatcagc gaacgcggac gggcaatcgg ctgcgatttg ctgccttccg
     2761 gcgcggtatg caggttctga taatcaaaat cgaacggctc gtaataatcg tcgatccccg
     2821 gcagatgcgg gttagcaaag attttaccca gcagcatggc tcggttaccc atgcgcggtt
     2881 gcagtttgcc attgatttta cggatccagc cgcctttgta tttttcctgg ttttcccagt
     2941 cagtcgggaa gccctggccc ggcttggttt ccacgttgtt gaaccacgcg tattccacgc
     3001 cttcacggct ggtccagacg tttttacagg taactgaaca ggtgtggcag ccgatgcact
     3061 tatcgagatt cagcaccatg ccgacttgtg aacgaatttt cattttacgc tctcctgtac
     3121 ctggtcattg ccttcgccat ctaaccagtc aatgttcttc atcttacgca ctacaacaaa
     3181 ctcatcgcgg ttagaaccta cggtgccgta gtagttaaag ccgtatgcca gatgggcata
     3241 gccgccgatc atatgcgtcg gtttcggcgt gatacgggtg accgagttat ggataccacc
     3301 acgctgttgg gtaatttccg aaccaggcag gttaacgata cgttcctgcg cgtggtacat
     3361 catggtcatc cctgccggaa cacgctggct gacaaccgca cgggcagtca gtgcaccgtt
     3421 gctgttgaag acttcaatcc agtcgttatc ggcgataccc agatctttgg catcggcttc
     3481 actcaaccag accaccggac caccgcgacc taaagtcagc atcagcaggt tgtcgctgta
     3541 ggtggagtgg ataccccact tctggtgcgg cgtcaggaag ttgagcgctt tttccgggtt
     3601 gccgttggat ttctggccta tcacttcttt caccgaacgg gtgtcgatcg gcggacgata
     3661 aaccagcagg ctttcaccga aatcacgcat ccactggtga tcctgataca gttgctgacg
     3721 accagagagc gtacgccatg ggatcagctc gtgaacgttg gtgtaaccgg cgttgtaaga
     3781 aacgtgttca tcttccagac cagaccaagt cgggctggag ataattttgc gcggctgtgc
     3841 ctgaatatcg cggaagcgga tcttctcgtc ttctttattc agcgccagat gcgtatggtc
     3901 acgaccggta aattcgctca gggcagccca ggctttcacg gctacctgac cgttggtttc
     3961 cggtgccagc gtcaggatca tctctgccgc atcaattgcg gtgttcagca tcggctggcc
     4021 ttttgccgga ccttccgctt tggtgtagtt gagcttacgc agcagatcca tctcgctctg
     4081 ggtgttccaa gcaatccctt taccgccgtt accgattttc tccatcagcg ggccgataga
     4141 ggtaaagcgt tcgtaagttg ccggataatc gcgctctacg accataatgt gcggtgcagt
     4201 tttgcctggg atcaggtcgc attcgccttt tttccagtct ttcacatcca gcggctgcgc
     4261 cagttcagcg gcagagtcgt gctggatagg cagcgtgacg atgtcggttt ctttacccag
     4321 atagccgacg cacacttcgg agaatttctt cgcgatggct ttgtagattt cccagtcgct
     4381 tttcgcttcc caggccggat cgaccgccgc agacagcggg tgaataaacg gatgcatatc
     4441 cgaagtattc atgtcgtctt tttcgtacca ggtcgccgtc ggcaaaatga tgtcggaata
     4501 gagacaggtg ctcgacagac ggaagtccag cgtaaccacc agatccagct tgccttccag
     4561 accattgtcc tgccagtcca cttcttccgg cttcacgccg ccctgttgcc ccagatcttt
     4621 accctggata ccgtgctccg tccccagcag gtacttgagc ataaactcat gacctttacc
     4681 ggaagaaccg agcaggttag aacgccagat gaacaggttg cgcgggtggt ttttaccgtt
     4741 ttctggttgt tctgccgcaa aacggatgga accctctttc agggatttca ccgtatagtc
     4801 caccggattc atcccggctt tttccgcttc gccagcgata gtcagcgggt tagtgcctaa
     4861 ctgcggtgca gacggcagcc agcccatgcg ttccgcacgg acgttaaagt cgatcaagtg
     4921 tccggtatag cgggatttgt ccgccatcgg tgacagcaac tcttccgccg tgacggtttc
     4981 ataacgccac tggctggagt ggttatagaa ataagaagtg ctgttcatgt gacgcgccgg
     5041 acgctgccag tcaagggcaa acgccagcgg ctgccagccg gtttgcggac gcagtttttc
     5101 ctgacctaca tagtgcgccc agccgccccc gctctgaccg acacagccgc agaaaatcag
     5161 catgttgatc agaccacgat agttcatatc gaggtgatac cagtggttca gccccgcacc
     5221 gacgataatc atcgaacgac cgtgcgtttt atcagcgtta tcggcaaatt cacgggcgat
     5281 gcgaataatc tggctgcgag aaacgccggt aatctgctcg gcccaggccg gggtataagc
     5341 tttcacatcg tcatagctgg ttgcacagtt aacgtcgttc aggccacgtt ccagaccgta
     5401 gtttgccagc gtcagatcat aaacggtggt caccagggcg gtgctgccat cagccagttg
     5461 caggcgtttc accggcagtt tgtgcagcag cacgttttcc agttccactt tgttgaagtg
     5521 ttccgtgccg tcgccaccaa agtacgggaa gcctacctca gcgatctcat cctggctacc
     5581 cagcaggctc agttgcagct cggtttcttc gccagttttg ccgtcgcgct gctcaagatt
     5641 ccatttgccc ttctcgcccc agcggaagcc aatagaaccg ttcggcgcaa ccatttcgcc
     5701 attggtatta aaggcgacag ttttccattc cggattgttt tcctggccca gcgcatcaac
     5761 cagatcagca gcgcgcagca tacgacctgc agcgtagtaa ccgtcgcgtt cttccagcat
     5821 caccagcatc ggcatgtcgg tgtagcgacg cacatagtcg gtgaaatact ggcttgggtt
     5881 gtcgaggtgg aattcacgca gcattacgtg gcccatcgcc agcgccattg ccgcatcggt
     5941 gccctgtttc ggtgccagcc acagatcgca cagtttggcg atttcagcgt agtctggtgt
     6001 gacggcaaca gttttggtgc ctttgtaacg cacttcagta aagaagtgag catccggggt
     6061 acgcgtctgc ggcacgtttg aaccccaggc gatgatgtag ctggagttat accagtcagc
     6121 agattccggt acgtcagttt gctcgcccca ggtttgcgga gacgcaggag gcaggtcgca
     6181 gtaccagtcg tagaagctta agcaagtacc gccaatcagc gagagatagc gtgcacccga
     6241 tgcgtaagaa accatcgaca ttgccggaat tggcgagaaa ccagcaacac ggtccgggcc
     6301 gtagtttttg atggtgtaaa cgttagatgc ggcgatcagt tcgttcacct cctgccaaga
     6361 agaacgaaca aatccaccgc gtccacgcgc ctgcttaaag cttttcgctt tatcggcgtc
     6421 ttcaatgata gaagcccatg cctcaaccgg atcgctatgc agcgccttcg cttcacgcca
     6481 cattttcatc aggcgtttgc gcatcatcgg gtatttcagg cggttggcac tgtaaagata
     6541 ccaggagtag ctggcaccgc gcgggcagcc gcgaggttca tggtttggca gatccggacg
     6601 ggtacgcgga tagtcagtct gctgggtttc ccaggtgacc agaccgtttt tgacgtagat
     6661 tttccagctg caggagccgg tgcagtttac cccgtgggta gagcggacga ttttgtcatg
     6721 ctgccaacgc tggcgatatc catcctccca gtcacggttg gtattgagaa gctggccatg
     6781 cccatcggca aaggtttcac ccttctgttt gaagtagcga aaccggtcca ggaatttact
     6841 catcggtttt ctcctgtggg agcctgtcgg ccctctgata aatcgacatt gctaatgttt
     6901 aagggcaagg taacgctctg aaacagcgtg ggaattgata acgatcaaga gtgatgggga
     6961 tgaaaaataa agtaaattcc taagataccc ctttaggata attaacagtt acagcataac
     7021 atgctgtaaa ttaagacgtt ttatacctgt agttatttaa actattaaga tttttatgca
     7081 aatgggtatt aaggagtatt ccccatgggt aacatattaa tggctcttaa tgaaatccgg
     7141 cgaaataagc catataacac taatatgttg taactaaatg acgcataaaa aaagcgcggc
     7201 atcatgccgc gcaaaggata atcaaatatt aagatttatt ttttagaatg ccgaccatat
     7261 accgcccaag taatcactac gcaggcgata tagaaaatca aaaatacttt cattgcacct
     7321 actggcgaac ccgttaatgc cagcgagcta ccaaacgctt tcgggataaa gaagccacca
     7381 atcgcgccaa tcgcagagat gaaacccagc gccgctgccg tgccggttgc cgcttcacgc
     7441 atcgcacgtt cgtcagaacc cccttctgct ttcacgcgat ccattgtcag tttacggaag
     7501 atcactgaaa tcatctggaa agtggaacca ctacccagcc cagctgtcag gaacagcgcc
     7561 aggaagaccg cgaagaacgc catgaagctt ccgccctgcc cgtcagtcgg taaggtcagg
     7621 aacagcaggc cgctgaaaat cgccatcaga ataaagttca ccagcgtgac acgagttccg
     7681 cccagacggt cagataatgc accacctgca gaacgcgcca gcgcaccaat aaacggcccg
     7741 aagaaagcgt attgcagaat ctgaacatcc gggaactgcg tttttgacag catcgcaaag
     7801 cccgcggaga agccgatgaa ggagccgaag gttgccagat acagcaggct cataatccac
     7861 agatgacccc gtttgagtac cggcaactgc tccttgatgg aggctttcga ggtagcaaga
     7921 tcgttcatgc caaaccacgc cgcaatggtg aagatggcaa ggaacggcac ccatatccag
     7981 gacgcattcg ccagatacag ctcagtccca tccggctgtt tgacgccctg gctaccaaat
     8041 actgcgaaaa tcgacagtga taccaccagc ggagcaacca actgcatgac gctgacgccc
     8101 atgtttccca gaccaccatt cagacccagc gcgccaccct gcttctgttt cggaaagaag
     8161 aagctgatgt ttgccatact ggatgcgaag ttcgcgccag caaagccgca tagcagagag
     8221 atgatgatga agacgctata aggcgtggag gtatcctgca cggcaaaacc cagccagacg
     8281 caaggaataa tcagaatacc ggtgctgaac gccgtccagc gacgaccacc gaagatagga
     8341 accataaagg agtatggaac acgtaataac gcgccagaaa ccgaaggcag cgcagtcaac
     8401 ataaatagct gatcggtcgt aaaattaaaa ccgactttcg gtaagttcac cgcaacagcg
     8461 ctgaacaaca tccatacgca aaacgccagc agcagacagg gaacggaaat ccacaggttg
     8521 cggctggcaa tacgttgacc gcgttgttgc cagaacgcag gatcttccgg tcgccaatct
     8581 gttatgacag ctccagtagc cctttcggag gcagatgagt gactcataga cacctctgat
     8641 actcgtttcg atgtctgcca ccttagtgtc tggagctaaa ggcaatttga tgtaaatcaa
     8701 acgataagat aactttatct atcattgata tttatcatta cccatagtga gtacagtgat
     8761 ttcataaaaa ttatgagatt tttcacggtg ctgtagaatc cctaccctta ccgatgtaaa
     8821 gcgactaacc acacggcaaa taaggagtaa ctctttccgg ctatgggtat acttcagcca
     8881 atagccgaga atactgccat tccagaatat atcgtcacat tcattaaggt tattgctcat
     8941 ttaaagcctg aaggaagggg tttacatgct taaacgttgt ctctctccgc tcaccctggt
     9001 taatcaggtt gcgcttattg tgttgctttc tactgctatt ggactggcag ggatggcggt
     9061 ttctggctgg ctggtgcaag gcgttcaggg cagcgcccat gcgatcaaca aagcgggatc
     9121 gctgcgcatg caaagttacc gtctgttggc ggcagtgcca ttaagcgaga aagacaagcc
     9181 cttaattaaa gagatggaac aaacggcatt tagcgccgag ttgactcgag cagcagaacg
     9241 agacggacaa ctggcgcaat tacagggttt acaagattac tggcgtaatg aactgatccc
     9301 tgcgctgatg cgtgcacaaa accgagaaac ggtgtcagcg gatgtcagcc agtttgttgc
     9361 cgggcttgat cagctggtat ctggttttga ccgcaccacg gaaatgcgta ttgagacagt
     9421 ggtactggtc catcgggtaa tggcggtatt tatggcactt ttactggtgt tcactattat
     9481 ctggttgcgg gcgcgactgc tacaaccgtg gcggcaactg ctggcaatgg cgagtgccgt
     9541 cagccatcgc gattttaccc aacgcgcaaa catcagcggg cgcaacgaaa tggcgatgct
     9601 tggaactgcg ttgaacaata tgtctgcaga actggccgaa agttatgccg tacttgagca
     9661 gcgggttcag gagaaaaccg ccgggctgga gcataaaaat cagatcctct cttttttatg
     9721 gcaggctaac cgccgtttgc attcccgcgc cccgctgtgt gaacgcctgt caccggtact
     9781 caacggctta cagaatttaa ccctgctacg tgatatcgaa ctgcgggtgt atgacactga
     9841 tgatgaagag aatcatcagg agtttacctg ccagccagat atgacttgtg atgataaagg
     9901 ctgccagctc tgcccgcgcg gcgtattacc cgttggtgat cgcggcacga ccctgaagtg
     9961 gcggctggct gactctcata cgcagtacgg tattttgctg gcgaccctgc cgcaggggcg
    10021 tcatcttagc catgatcaac aacaactggt ggataccctg gttgaacaac ttaccgccac
    10081 gctggcgctg gatcgccatc aggaacgtca gcaacagttg atcgtgatgg aagagcgtgc
    10141 caccattgcg cgcgaactgc atgattctat tgcccaatct ctctcttgca tgaagatgca
    10201 ggtgagttgt ttacagatgc agggcgatgc gctgccagaa agcagccgcg aactgttaag
    10261 tcagatccgt aacgaactga atgcatcctg ggcgcagttg cgtgaattgc tcaccacatt
    10321 ccgcttgcag ctcaccgagc ctggattacg tccggcgctg gaagcgagtt gcgaagagta
    10381 cagcgccaaa tttggcttcc cggtgaagct ggattatcaa ttgccgcctc gtctggtgcc
    10441 ttcacatcag gcaatccact tgttgcaaat tgcccgtgag gcattaagta acgccctcaa
    10501 acattcgcaa gcgagtgagg tcgtggtgac ggtggcgcaa aacgataatc aggtcaaact
    10561 gaccgtccag gataacggct gcggcgtgcc tgaaaatgcc atccgcagca atcactacgg
    10621 catgataata atgcgcgacc gtgcgcaaag tttacgaggc gattgccgcg tccgccgtcg
    10681 tgaatcaggt ggcaccgaag tggtggtcac ctttattccc gaaaaaactt tcacagacgt
    10741 ccaaggagat acccatgagt aatcaggaac cggctactat cctgctgatt gacgatcacc
    10801 cgatgctgcg aactggcgta aaacagctta tcagtatggc accagatatc accgtggttg
    10861 gcgaagcgag taatggcgaa cagggtattg aactggcgga atctcttgat cccgatctga
    10921 tcctgttaga tctcaatatg cccggcatga acggtctgga aacgctggat aaactgcgcg
    10981 aaaagtccct ctcagggcgc attgtggtat tcagcgtctc taaccatgaa gaagatgtgg
    11041 tcaccgcact gaaacgcggc gcggatggct atctgttaaa agatatggaa ccggaagatc
    11101 tgctgaaagc attgcatcag gcagctgctg gcgaaatggt attaagcgaa gcattaacgc
    11161 ctgttctggc cgccagcttg cgcgctaacc gtgccactac tgagcgcgat gttaaccagt
    11221 taaccccacg cgagcgcgat attctcaagc tgattgccca gggtttgccg aacaagatga
    11281 ttgcccgccg cctggatatc accgaaagca cagtaaaagt gcacgtcaag cacatgctga
    11341 agaaaatgaa gctcaagtct cgcgtggaag cagcggtatg ggtgcatcag gagcgcattt
    11401 tctgattacg gttcccagcg cagttcgtcg tttgacaatg catcgaacgg ttcgacaagc
    11461 gttagcgtga tctcattgga ggagacacgc tgcccctggt tatcttccac caccacagac
    11521 aatcgccagt gattgcttgc cccttccccg ttctgccagt caggcatgat cagcgtccag
    11581 ccctccgcgc tgttggcttg tgcgcccggc gtcaaactta atatctgcgt atcgccctgc
    11641 caaatcagtt gccgaatacc gtaacgactg cggatttgta atttcagcgg cactgtttcg
    11701 ccaggtttta gatcccacgg cggtgtcgcc agaaacaccg ttaacgtttt tcgctgtcgg
    11761 tactcaagag tcggtagatt atttcgctgc ggattgtcat agcgactacc acgtaacgac
    11821 tgactttcgg caacctcgcc cgcagaaagt tgttttttga gcggtacacc aaagcggtaa
    11881 ttaagattca gcccgaggtt attttgattt tcgccacttt ccccctgttt atgctgggcc
    11941 gtcacagtga ctaatggcac aggggtgtaa tttaatccca gactcaacgc gacgggattg
    12001 tgataacccg taccagagtt aaacaaatca acacgatcac caaaatactg ttctaggctg
    12061 acactggtat tgaggtgttg atagaacggc atgcgcatcc gagctgtcag gtcgtacccg
    12121 cgcgccatcc gttgttcctg cgtggctgtc tgttcatgcc atgcagcaaa cggctgataa
    12181 aagtttgccg ataatcgcaa atattcgccc cacgcttcgg caccaaagcc cgctcgctga
    12241 agattttcgt cctgcaagtt gtcataaaaa gtgttataac ccaccagcca gttgccgcgc
    12301 gcccagcgtt gcccaacgcc cacattgctc accaacccat tatcctgctg agtaagacca
    12361 agctggctcc aggtgagata acgatcatta tcttgtaacg gcacaaacca gcttccacga
    12421 ctgccggtga aatgtccttc gttatccact ttgacgtcaa cactggcatt tccccacggt
    12481 gatagccagg actctacgtg ctgattaacc tgttgactaa gcgcgtcgcg gacttttccc
    12541 aatgcgaaag cttttgcctg ctcgccagta tccagcccgt tatcattcat actggtttcg
    12601 ccgaaatctt tcacaatttc agcaaagtgt ttttccccat catgattttc gggagccatg
    12661 cctaaatccg gcagaccatc attgttatta tcaaagggat ttgccgcttt ttgctcgaag
    12721 gtagattgtg cgttagctgt accgcctgct acaagcaaga gtaaataaaa cgggataatg
    12781 cgaggaacga aacggctcaa aacgtcggta acttccggta ataatgaaaa accatagcct
    12841 taaagaataa cgattactgg caacttttgt cgtattttct cgcgctttca ggaaaatcct
    12901 taaaaatgtg accgattggt cagacgctgc agaagcagta agatagaaga tgagtgtcta
    12961 attccctgaa tacaggagta aataatgcaa aaaatcgtga tcgttgccaa tggcgcacct
    13021 tacgggagcg aatccttgtt taacagcttg cggctggcca ttgcgttacg agagcaggag
    13081 agcaatctgg atctgcgtct gttcctgatg tctgatgcgg tcacagccgg gttgcgcggg
    13141 caaaaaccag gggaaggcta caacattcag caaatgctgg agatccttac cgctcagaat
    13201 gtaccggtga aattgtgcaa aacctgtacc gacgggcgcg ggattagcac acttcctctg
    13261 attgatgggg tggaaatcgg tactcttgtg gaactggcgc aatggacgct gtcagccgat
    13321 aaagtgctca cattttaatg tttaaggggg cgtctgacgc cccactatga gggaagttta
    13381 ctcaggcgaa tcccggcctt aacacaccat ccggataatt ctccgccagt agttttttta
    13441 ccgataccag caaatcattc agcccatcgt cctgcattcc cagtttgata agctcctgtt
    13501 ccagtgaaaa caggtattgt gcattggttc ccagcggacc gctcgccgcc gcaatcaacg
    13561 gcgcgatgac ctgagcgcga gtgtcagatt catattctgg atgtcgcggg tccataataa
    13621 acacaatggc gtttactgtg cgtccatcat caagatcaag ctgacaccag gttggcagat
    13681 aacagccggt aatcatctcg cgcttccaca acagggttag ttcctgctcc agcgtctctt
    13741 ctggtagtcg ataggcgacg cctgtggtgc gtccgccctc tttcagtgca agcatccgtc
    13801 ccggctggtg cgcagtcccg cgcccggcgg tcaggcgcag gcaaaacgcg cgatgccatc
    13861 caaccagtgt accggtgcat gattcggtaa actccagtgc cggattccac atcaacgatc
    13921 cataaccgaa gatccacacc ggtccctcat caggtcgaca agccagcgtc gccgccagcg
    13981 aagccgcccg ttgttctgct gaccataaga gtgattcttc aatggcacca aatgccgttt
    14041 tacaatcggc attcatcaag aaatcacgcg ttatcacctt acacctccgc cactgcatac
    14101 ttccctgcga caactttttg gtgtcttccg gacattttta ccgctcattt tgaaaccact
    14161 aacggaacaa tatgcaatcc acggaccact tgcaaggcaa caccgcacga atagctttaa
    14221 ctaagctacc ggttttacga ttttttatgc catttatcat catccccttt ggcatattca
    14281 tgcttcacag cagcccaggc cactttatgc gcggtttctt cgcgactggc gtcatcacgc
    14341 cgatcttctt tatctttata ttgatcccat gcgctgttga acgcttcttt atagatatcc
    14401 tgggcatgag acggtagaac gtgctttacg ctttccggca gatcgctttt cgttttatac
    14461 ggcatcgttg actcctttta caaagttgaa cattaagtgt ggtcaacaat atctactctt
    14521 gccaaaacat aagaattttc atgtcagact gttcttaaga aacgctaaaa caaacattgt
    14581 ctaaacgtaa tacttatttt tatgccccaa tgagtaaaaa atcgacagcg gggtaaaaat
    14641 tcgtaaaatg ttaccgatat ccagacactt tctgttattt ttcctaccta ttttttacat
    14701 tttcgataaa ttcatctgca aagggataac cataatgtca aatgctcaag aggcggtaaa
    14761 aacccgccac aaggagactt cgcttatttt cccggttctg gtgctggtag tgctgttcct
    14821 gtggggaagt agccagacac taccagtggt cattgccatc aatcttcttg cgcttattgg
    14881 tattttaagt agcgccttta gtgttgtccg tcatgcggac gtattagccc atcgcctggg
    14941 agaaccttac ggttcgctta ttcttagcct ttcagtggtt attcttgaag tcagtttgat
    15001 ttcagcttta atggcaaccg gcgacgccgc gccaacgcta atgcgtgata cgctctattc
    15061 aatcattatg attgttaccg gtgggctggt tggcttttca ttattgttgg gcggtcgtaa
    15121 gtttgccacc caatatatga atctgtttgg tatcaagcag tatttaattg cgctgttccc
    15181 cctggcgata atcgtactgg tatttccaat ggctctgcct gcggcgaatt tttcaaccgg
    15241 tcaggcgtta ctggtagcat taatttctgc ggcaatgtat ggcgtatttt tgctgatcca
    15301 ggccaaaacg catcaaagtt tgtttgtcta cgagcacgaa gatgacagtg atgacgacga
    15361 cccgcaccac ggtaaaacgt ctgcccatag cagcctgtgg catgctatct ggttgattat
    15421 ccatctgatt gccgttattg cagtcaccaa aatgaacgcc agctcgctgg agacattgct
    15481 cgacagcatg aatgcccctg ttgcctttac tggcttcctg gtggcactgt tgattctgtc
    15541 gccggaaggt ttaggtgcgt taaaagcagt gttgaacaac caggttcagc gcgcgatgaa
    15601 tctgttcttt ggttcagtgt tagcaacaat ttcgcttacc gtacctgtcg tcacgctaat
    15661 tgcctttatg acgggtaacg aattgcagtt tgcacttggt gcgccagaaa tggtggtgat
    15721 ggtggcctct ttagtgctgt gccatatctc cttctccacc ggacgtacta acgtgctcaa
    15781 tggcgcagcg catctggcac tgtttgccgc ctatttgatg acgatatttg cctgacagtt
    15841 atatctcctc cgatgacgga aatcgggaga gataagataa attcctgctt attgtatttc
    15901 ttcatactac ctggacagct cgttaatcag aattgcgagc ctgtaccaca acctgcacga
    15961 aaccactgca acagctgttg aaaagccgga atgtactctt tattgcaaac atttcaggga
    16021 tgaagtgaag atgaattgag acgtagtatc cacaccaagc cggaattatc cggtgaggcg
    16081 caatgttgcg ggggctttat ccctggtggc attggttgct ggaaagagaa aacccccgca
    16141 cgttgagagg taaaacctga caacaaaacg ggggctaatc ttgaaaccag acacttcaag
    16201 aatagccgca aaatgttgtc attacaacaa ggcgactata tgacgctcgc gcagtttgcc
    16261 atgactttct ggcacgacct ggcagcaccg atcctggcgg ggattattgc cgcagcgatt
    16321 gtcagctggt ggcgtaaccg gaagtaaggt gaaaacgggc gtcaggctgc cgtgatggca
    16381 atatgcgccc gaaaccggcc gcaggggtaa cagcctgcgg ccttttttgt atctggcacc
    16441 catgaaaaaa gtcttaacgc agaacgctaa gactttattt ttcaagcaaa aaagattact
    16501 tgctggtatc cagttcttcg aaacctttca ccagatcatc aatcgctttc atctgcttga
    16561 ggaacggttc cagtttagcc agcggcagcg cggatggacc atcacatttc gcatgttccg
    16621 gatccggatg cgcttcaata aacagccccg ccagacctac cgccataccg gctcgtgcca
    16681 gctcagccac ctgagcacga cgaccaccgg aagctgcgcc aaacggatcg cggcattgca
    16741 gtgcgtgggt cacgtcgaaa atcaccggcg agttaccaga cactttcttc ataatgctga
    16801 agcccagcat atcgacaacc aggttgtcat agccgaagtt agcaccacga tcgcaaagaa
    16861 tcactttttc gttgccgcct tctttgaatt tatcaacgat attacccatc tgtcccgggc
    16921 tgacaaactg tggtttcttg acgttaatta ccgcaccggt tttcgccatg gcttcaacca
    16981 ggtcagtctg gcgagcaaga aacgccggca actgaatcac atccacgaca tcagcaacgg
    17041 gctgtgcctg acttggttcg tgaacgtcgg tgataatttt cacgccaaaa gtctgcttca
    17101 actcctggaa gattttcatc ccttcttcca ggcccggtcc acgataagag tggatggagg
    17161 agcggttggc tttgtcaaaa gaggctttga acacgtaagg gatacccagt ttctgggtca
    17221 cagttacgta gtgctcgcaa atgcgcatcg ccagatcgcg agattccagc acgttcatac
    17281 cgccaaacag tacgaacggc aggtcatttg ctacgttgat gtcgccaatg ctaaccactt
    17341 tttgtttcat aggatcgcct taatcttgag taaaatgtcg attaattaat gcagcacaat
    17401 atgtttatgc gcgatgttat ttatttgcgc acggatcatt tcgctgatcg ggtcttccgg
    17461 acactgttca acgaaataac ttaaatcgtt caacgcaacg tgttcgcaat ccagttgcgc
    17521 ataaatcaac ccgcgatcgc gaatttcata gggatcttca gggttgaatt gtaataaagc
    17581 ttcgctggtg cgtaacgcca gctccatctg attttcttcc atcaacgagg ctttgagtgt
    17641 atccagcaat ttgcggatta cctcaatgtt atcagcttca tcaaggtctt cataaaacag
    17701 ttccgccgac gggctgatat ttccctttaa ccatccgtcc agcatatgtt cgcttaacga
    17761 ttcaccgtta aaaggattaa tcagccaaat ttcgccatcc ggacattcaa tgcgcaatat
    17821 cagctgcgta gggaaaatca ccggcagcag cggcaaatcg agacgattcg cgacccataa
    17881 taaaaccgca cctaatgata ccgcactgcc ctgtcgattc tttaacacct ggtccagcca
    17941 taatgcatcg gaaagacgat aaacgccgcg tgaggcttta aatccccagt cgccgtaaaa
    18001 cagcgcgatc agtttttcca gctgctcttc taaaggcaga agctggctga tttcttcctt
    18061 cgccagacta acgagacgct ccagttcgtc gtaaacatct tgcgagggaa aatcgcggcg
    18121 gattgcttcg caagccagga tcatgccttc gcacaatggc gctttattaa attcgaaatc
    18181 agctaacgat ctcatgactt accccagtaa cggtactttt gtggtggcga gtttaatgat
    18241 gatgtacagc accaccaacg ccagcgggaa ggcaataatg cgcgcctgct gactgtgcat
    18301 acgacgataa tcgagtgcaa taaaacccaa aacgatataa atgataactc caaacagctt
    18361 ttcagtcagc cactgtgcct gcccggaaaa tggcaggatg tgcgctttag ccatcaacgc
    18421 aatgccgctt aacaataaca ccgtatcgac aaccggcggc acaatgcgcg tccagcgagc
    18481 aaatgcctgt ggatgcttct gataacgtag ccagaaacgt aaggttaata gcccaacaga
    18541 aagcgcgata ctaataagat gaacactaag cagtgtagaa aaacttgtca ttgataatag
    18601 cggccgagcg ttacgcgctc gttatcacca tagtcacggc aggtttcgac gtcatgatac
    18661 cccgcgagga taaatgcttg tcgcaccgct tcgccctgct gccagccatg ttccagaagc
    18721 agaaagccgc cggataccag cgcgttacgc gactgttcga tgatatgcac gatgtctgcc
    18781 attccactgt ctgccgcaac cagcgcagtg agcggctcaa agcggacatc gccttgttga
    18841 agatgtgggt cctgctcgtc aatatacggc ggattgctga caatcatcgc aaactgctgc
    18901 ccggctagcg cgctaaacca gtcgctttgc agaatgtgga tatttttgat cgccagatgc
    18961 tgggcattac gttgtgccag ggagacagca tcaggcatac gatctacagc gataatttcg
    19021 cagtccgggc gctcgctagc cagcgcaagc gcaatcgccc cggtacccgt cccgagatcg
    19081 agaatacggc aaggttgttc aggcaaccgc gccagtgcct gctccaccag acactccgta
    19141 tccgggcgcg gaattaaggt cgctggcgaa acaaataacg gcaacgacca gaattctcgc
    19201 accccggtta aatgagcaat gggttcacca tcgcgacgac gtgtcagtag cgcatcaagt
    19261 tgctgacatt gttcgtcagt cagctgcgtt tcaccaaagg cgagaataaa agtacgccct
    19321 ctgccggtaa catgctccag caggatttca gcatcacgcc gcgggctttc gctcgcctga
    19381 agttggctta ttgcttcacg taaccagtgt tgatattcca ttattcctgc tcggacaacg
    19441 ccgccagttg gtcggcctga tgttcctgga taatcggttc aatcagcata tccagcttac
    19501 cttccatcac ttcatccagg cggtagagcg tcaggttgat gcggtgatcg gtaacgcgcc
    19561 cctgcgggaa attgtaagta cggttacggt cgctgcgatc gccactcccc agcaggttac
    19621 gacgggtaga cgcttcggcc tgttggcgtt ttgccatttc agcagcgtgg atgcgagcac
    19681 cgagcactga aagcgccttc gctttgtttt tatgttgtga acgttcgtcc tgacattcaa
    19741 caacaatccc ggtcggcaag tgagtaatac gaattgccga atcggtggtg ttaacgtgct
    19801 gaccacccgc ccctgacgag cggaaagtat caatgcgtaa atctgctggg ttgatgtccg
    19861 gcagttctgc gtcaggcagt tctggcatta ccgcaacggt acaagcagaa gtatgaatac
    19921 gaccctgcga ttccgtagca ggaacacgtt gcacgcgatg accgccggat tcaaatttca
    19981 gacgaccata cacaccatca ccgctaattt tggcgatgat ctctttataa ccaccatgtt
    20041 caccctcgct ggcgctcatg atttctaccc gccagcggcg ggcttcggcg taacggctgt
    20101 acatacggaa cagatcgcct gcaaacagcg ctgcttcgtc gccgccggtt ccggcacgga
    20161 cttcgaggaa ggcgttacgt tcgtcatcag gatcttttgg tagtaataga acctgtaatt
    20221 gctgttccag ttgctcgctt ttttctttag cttcgcgcag ttcatcctgc gccatctcac
    20281 gcatttcagg atcgtcgagc atcatctgtg cggtttcgat atcttcctga acctgttgcc
    20341 agtcggtaaa acagcgcgaa acatcactta actgcgcata ttcgcgtgat aatgcgcgaa
    20401 aacgttcctg gtcggcgata gtttgcgcgt cacccagcaa cgcctgaact tcttcatggc
    20461 gttcatgcag ggcttccagt ttggcaacga tagaaggctt cataggcgta aatgcaccct
    20521 gtaaaaaaag aaaatgatgt actgctactc cagcccgagg ctgtcgcgca gaatattcag
    20581 gcgttcgtta tccccgtcac gggcggcctg ttgaagtgat ttcgttggcg catggatcaa
    20641 gcggttagtc agtttccatg ccagatcctg cataatggct tgcgcgtcgc cgccctgctc
    20701 aagggccgct aacgctttgg cggttaactc atcgcgaacg tgctctgcct ggctgcgata
    20761 ctcgcgaatg gtttcgctgg cgctttgtgc tcgcagccac gccataaatt cgctggtttc
    20821 ctgagcgaca atagtttccg cctcaaccgc tgcggcttta cgctgcgcca ggttgtgcga
    20881 aatgatgctt tgcagatcgt ccacactata aagataagca ttcgccagtt tgccaacttc
    20941 cggctcaaca tcgcgcggaa cggcaatatc caccaacagc attggttggt tacgacgact
    21001 ttttaatgcg cgctccacca tgcctttccc gataatcggt aacgggctgg cggtggaact
    21061 gatgatgata tcggcttcgc gcagacgttc gtcgatctca ctcagggcaa tcacttccgc
    21121 gccaacttca tctgccagaa tttgggcacg ttcgcgagtg cggttggcga taatcatctt
    21181 ctgtacttta tgttcgcgca gatgacgcgc taccagctcg atggtttcgc ccgcgcctac
    21241 cagcaacact gtgaccgtag agagcgattc aaagatctgc cgcgccagcg tacaagccgc
    21301 aaaagcgaca gacacagcac tggcaccgat atctgtttca gtgcgaacgc gtttcgctac
    21361 agagaaagat ttctggaaca tgcgttccag ttcgctggcc ttcatatgac ctttttgcga
    21421 atcgacaaac gcttttttaa cctgaccgag gatctgcggc tccccaagaa ccagtgaatc
    21481 caggccgctg gcaacacgca ttaaatggct aaccgcgtcg ttatcctgat gccagtagag
    21541 gcttttacgc agatcttctt cattaagatt gtgataatcg caaagccagc ggattaacgc
    21601 ctcttgcagg ttatcctgct cttcaacgct aagataaagt tccgtgcggt tgcacgtcga
    21661 cagcaccacg ccgccctgca ccatcggctg cgcaagcagg ctgtcaagcg cctgatcgag
    21721 cttatccggc gaaaacgata cacgttctcg cagcgataca ggtgccgttt tatggttgat
    21781 accgagtgct aaaagggtca tgtctgcggg aaataatacc aacgttgata gggttagtct
    21841 gcttgcatca tacaggatgc gtaagatcaa taaaagagag cgcccccttt tggagtaatt
    21901 gccgaaagcc gttagattct ggcaattaag acaacttgaa catagacgat agcggacggt
    21961 aacgctagca ttaagggtta taactgcaac gtatctcaag gacttgtcat cactatgccc
    22021 ctgcccgatt ttcgtcttat ccgcctgcta ccgctggctg ctcttgtgct cactgcctgt
    22081 tccgttacca cgcccaaagg tcctggcaaa agcccggatt cgccacaatg gcgtcagcat
    22141 cagcaagacg tgcgcaatct taatcagtat cagactcgcg gcgcgttcgc ttatatttct
    22201 gaccaacaaa aagtgtacgc ccgctttttc tggcagcaaa ccggccagga tcgctaccgt
    22261 ctgctgctca ctaacccatt gggcagcacg gaactggagc tgaatgctca accgggtaac
    22321 gtgcagttag tcgacaataa aggtcagcgt tataccgccg atgacgccga agagatgatt
    22381 ggcaaattga ccggaatgcc aattccgctc aacagcttgc gccagtggat tttaggttta
    22441 ccgggtgatg caaccgacta caaactggac gaccagtacc gcctgagcga aattacctac
    22501 agccagaatg gcaaaaactg gaaggttgtt tatggtggtt atgacaccaa aacgcaacct
    22561 gcgatgccag ccaatatgga actcaccgac ggtggtcaac gcatcaagtt aaaaatggat
    22621 aactggatag tgaaataatg cggacacagt ggccctctcc ggcaaaactt aatctgtttt
    22681 tatacattac cggtcagcgt gcggatggtt accacacgct gcaaacgctg tttcagtttc
    22741 ttgattacgg cgacaccatc agcattgagc ttcgtgacga tggggatatt cgtctgttaa
    22801 cgcccgttga aggcgtggaa catgaagata acctgatcgt tcgcgcagcg cggttgttga
    22861 tgaaaactgc ggcagacagc gggcgtcttc cgacgggaag cggtgcgaat atcagcattg
    22921 acaagcgttt gccgatgggc ggcggtctcg gcggtggttc atccaatgcc gcgacggtcc
    22981 tggtggcatt aaatcatctc tggcaatgcg ggctaagcat ggatgagctg gcggaaatgg
    23041 ggctgacgct gggcgcagat gttcctgtct ttgttcgggg gcatgccgcg tttgccgaag
    23101 gcgttggtga aatactaacg ccggtggatc cgccagagaa gtggtatctg gtggcgcacc
    23161 ctggtgtaag tattccgact ccggtgattt ttaaagatcc tgaactcccg cgcaatacgc
    23221 caaaaaggtc aatagaaacg ttgctaaaat gtgaattcag caatgattgc gaggttatcg
    23281 caagaaaacg ttttcgcgag gttgatgcgg tgctttcctg gctgttagaa tacgccccgt
    23341 cacgcctgac tgggacaggg gcctgtgtct ttgctgaatt tgatacagag tctgaagccc
    23401 gccaggtgct agagcaagcc ccggaatggc tcaatggctt tgtggcgaaa ggcgttaatc
    23461 tttccccatt gcacagagcc atgctttaag ccgggcaagc tgagtttcgg tgacaacgtc
    23521 accttgttcc agacgttgca tcgcgctctt taatacaccg cctggaaagg atcatgcctg
    23581 gcccgcacag ttttcggcag attctttcca ccaatggacg catgcctgag gttcttctcg
    23641 tgcctgatat gaagcttttt gctggtaacg ccaccccggt actagcacaa cgtattgcca
    23701 accgcctgta cacttcactc ggcgacgccg ctgtaggtcg ctttagcgac ggcgaagtca
    23761 gcgtacaaat taacgaaaat gtacgcggtg gtgatatttt catcatccag tccacttgtg
    23821 cccctactaa cgacaacctg atggaattag tcgttatggt tgatgccctg cgtcgtgctt
    23881 ccgcaggtcg tatcaccgct gttatcccct actttggcta tgcgcgccag gaccgtcgcg
    23941 tccgttccgc tcgtgtacca atcactgcga aagtggttgc agacttcctc tccagcgtcg
    24001 gtgttgaccg tgtgctgaca gtggatctgc acgctgaaca gattcagggt ttcttcgacg
    24061 ttccggttga taacgtattt ggtagcccga tcctgctgga agacatgctg cagctgaatc
    24121 tggataaccc aattgtggtt tctccggaca tcggcggcgt tgtgcgtgcc cgcgctatcg
    24181 ctaagctgct gaacgatacc gatatggcaa tcatcgacaa acgtcgtccg cgtgcgaacg
    24241 tttcccaggt gatgcatatc atcggtgacg ttgcaggtcg tgactgcgta ctggtcgatg
    24301 atatgatcga cactggcggt acgctgtgta aagctgctga agcgctgaaa gaacgtggtg
    24361 ctaaacgtgt atttgcgtac gcgactcacc cgatcttctc tggcaacgcg gcgaacaacc
    24421 tgcgtaactc tgtaattgat gaagtcgttg tctgcgatac cattccgctg agcgatgaaa
    24481 tcaaatcact gccgaacgtg cgtactctga ccctgtcagg tatgctggcc gaagcgattc
    24541 gtcgtatcag caacgaagaa tcgatctctg ccatgttcga acactaatcg aacccggctc
    24601 aaagacccgc tgcggcgggt ttttttgtct gtaatatcca tttgtatgac ctatgcctcc
    24661 ttcacctgcc atttagttga cagatgatgc gctcacggat gaaacattat tgtgaacaaa
    24721 atattttcct cacatgtgat gcctttccgc gctctgatcg acgcttgctg gaaagaaaaa
    24781 tatactgccg cacggtttac ccgtgacctg attgccggga taaccgtcgg gattattgct
    24841 atcccgctgg cgatggcgtt ggctattggt agtggtgtgg ccccccagta cggtttatat
    24901 accgcagctg ttgcggggat tgtcattgct ctaacgggtg ggtcacgctt tagcgtttcc
    24961 ggtccgactg cggcatttgt ggtaattctc tatcccgttt cgcaacagtt tggactggca
    25021 ggactgctgg ttgcgacctt gttgtcggga atctttttga ttctgatggg gctggcacgc
    25081 tttggtcgtc tgattgagta tattccggtt tccgtcacct taggtttcac ctcgggtatc
    25141 gggatcacca tcggtaccat gcagattaaa gattttctcg gtctgcaaat ggcccatgtc
    25201 ccggaacatt atctacaaaa agtcggcgca ttatttatgg cgctgccgac cattaatgtg
    25261 ggtgatgctg ccattggcat tgtgacgctt ggcattctgg ttttctggcc gcgtctgggc
    25321 attcgtttac ccggtcacct tccggccttg ctggctggtt gcgcggtgat ggggattgtt
    25381 aacctgctcg gcggacatgt tgctaccatc ggttcgcaat tccactacgt cctggccgat
    25441 ggttctcagg gtaacggtat tccgcaactg ctgccgcaac tggtgctgcc gtgggatctg
    25501 cctaattcag aattcacgct aacctgggat tctattcgca ccctgctgcc tgcggcattc
    25561 tcaatggcaa tgctcggggc gatcgaatct ctgctctgcg ctgtggtact ggatggtatg
    25621 accgggacta agcacaaggc gaatagtgaa ctggtcggtc aggggctggg gaatatcatc
    25681 gctccattct ttggtggtat taccgctact gctgccatcg cgcgttctgc cgctaacgtc
    25741 cgtgccgggg caacttcccc tatctcggcg gtgatccact ctattctggt tattctcgcc
    25801 ctgctggtac tggcaccact gctctcctgg ctgccgcttt ccgccatggc agccctgctg
    25861 ttgatggtgg cgtggaacat gagtgaagcg cacaaagtgg tcgacttgct gcgtcatgcg
    25921 ccgaaagatg acatcatcgt catgctgctg tgtatgtcac tgaccgtgct gtttgatatg
    25981 gttattgcca tcagcgtggg gatcgtgctg gcatcgctgc tgtttatgcg tcgtatcgca
    26041 cgtatgactc gcctggcacc ggtagtcgta gatgttccag acgatgtcct ggttctgcgc
    26101 gttattggcc cgctgttttt tgctgctgct gaaggcttat tcacggacct ggagtcacgt
    26161 cttgaaggca aacggattgt gattctgaag tgggatgccg ttccggtact tgatgctggt
    26221 ggtcttgatg cgttccagcg ttttgtgaag cgtctgcccg aaggatgtga actgcgcgtg
    26281 tgcaacgtgg aattccagcc actgcgaact atggctcgcg caggcattca accgatcccg
    26341 ggacgcctcg cgttcttccc gaatcgtcgc gcggcgatgg cggatttata agtacgagat
    26401 tgaccagtca gcgcaaactg actggtcagc aaactgcatt atttgttagc tatgacggcg
    26461 attatcgcta cggcggcaac gtttgtcata gtgacgaacc caccagtaac ggtcgcacac
    26521 ctgttcatgc ccgccaacac gggcacccgc cagccagaga atggcaccga cgaaaatact
    26581 tagcactgca ccatgtgcga aatactgggg catattaaac tgtggtaact ggttgaggat
    26641 tgaatacccc acgccgacca ccattaccac cagacccaac cccatgagca cgttaccgag
    26701 tatcgaagcg tttttgcgtt tcatatgtca cctccggaac tttctgggtt gtaacaggga
    26761 atacccctct tccttatgtg taaagtatag acaacaccct gtgggttatg tgcgggcgta
    26821 atcacaatta caacccttat ttcaacaaaa ctttacaaat aaacgcctga acaccttgac
    26881 tttctaatag tccactcagg caattattac gttatctgca tttgatacgc agttttttgt
    26941 caagcggggc cgcaaccagt aaactacgcg ccagttatgt acacactcag gacaaaaaaa
    27001 cgtgacgatt aaattgattg tcggcctggc gaaccccggt gctgaatacg ccgcaacgcg
    27061 acataatgct ggtgcctggt tcgttgactt actggcagag cgtttgcgcg ctccgctgcg
    27121 cgaagaggct aaattctttg gttatacttc gcgagtcacc cttggaggcg aagatgtccg
    27181 cctgttagtc ccgactacat ttatgaatct cagcggcaaa gccgttgcgg cgatggccag
    27241 ttttttccgc attaatccgg acgaaattct ggtggcccac gacgaactgg atctgcctcc
    27301 tggcgtcgcc aaatttaagt tgggcggtgg ccatggtggt cacaatggac tgaaagacat
    27361 catcagtaaa ttgggtaata accctaactt tcaccgttta cgcatcggaa tcggtcatcc
    27421 gggcgataaa aataaagttg tcggttttgt gttaggcaaa ccgcctgtta gtgaacagaa
    27481 gttaattgat gaagccatcg acgaagcggc gcgttgtact gaaatgtggt ttacagatgg
    27541 cttgaccaaa gcaacgaacc gattgcacgc ctttaaagcg caataagtcg ttgtctgcgg
    27601 catttttgcc gagtgccgtg tataataggc aaagttattt ccatttctgc aatctgttag
    27661 caataacagg ttgattatta agatattaag gtgatttaaa tcatgggatt caaatgcggt
    27721 atcgtcggtt tgcccaacgt cgggaaatct accctgttca acgcgctgac caaagccggt
    27781 attgaagcgg ccaactttcc attctgcacc attgagccga acacaggcgt cgtaccaatg
    27841 cctgaccctc gcctggatca actggctgaa atcgtaaaac cgcaacgtac gcttcccacg
    27901 accatggaat ttgtcgatat cgccggtctg gtaaaaggcg catcgaaagg cgaaggtctg
    27961 ggtaaccagt tcctgaccaa catccgtgaa accgaagcga tcggtcacgt tgttcgctgc
    28021 tttgaaaatg acaacatcat tcacgtttcc ggcaaagtta acccggctga cgatattgaa
    28081 gttatcaaca ccgaactggc gctggcagac ctcgacacct gcgaacgtgc gattcatcgc
    28141 gtacagaaga aagccaaagg tggcgataaa gacgcgaaag ctgagctggc ggtcctggaa
    28201 aaatgcctgc cccagctgga aaacgcaggt atgctgcgcg cgctggattt aagcgctgaa
    28261 gagaaagcgg ctattcgtta cctgagcttc ctgacgctga aaccaacaat gtacatagcc
    28321 aacgtcaacg aagatggttt tgaaaacaac ccatatcttg accaggtgcg tgaaatcgcg
    28381 gcgaaagaag gttctgttgt ggttccggtt tgtgctgctg ttgaagcaga cattgccgaa
    28441 ctggacgacg aagaacgtga cgagtttatg caggagcttg ggctggaaga accgggcctg
    28501 aaccgtgtga tccgtgccgg ttataagctg ctgaacctgc aaacttactt caccgctggg
    28561 gtgaaagaag tgcgtgcatg gaccattccg gttggagcaa ccgcgccgca ggcagcgggc
    28621 aaaatccata ctgattttga aaaaggcttt atccgtgcac aaaccatctc gtttgaagat
    28681 ttcatcactt acaaaggtga acaaggcgcg aaagaagcag gcaaaatgcg tgcagaaggt
    28741 aaagattaca tcgtgaaaga tggcgatgtg atgaacttcc ttttcaacgt ctaattaatt
    28801 tattgtctca tgaggtttcg ctaaatctca tgaagagcgc aaaagtatat aaaatccacg
    28861 caattgcgtg gatttttcat ttcaccgagt cctaatcatt cttgtaacat cctacacttt
    28921 tgtgcttatt gcctacagtg aaccacgaga ataattaaag acataagtgt tcagatgact
    28981 gatatctcat tagcagtgat ataacgtcag tttttgttcc actacacatc agccctcttc
    29041 acagctgatc aatattgatc tcgctccaat atgctggcga gaaaaggact tacctgaagg
    29101 gtaatatccc gcagataaag tccccgaata acgtgattac gtgcgatctt ggcaagatga
    29161 aaatgataac cagtagcaca ataataacga taggccagaa aatccattga acaaaaatga
    29221 atatcgatgt tttttgcttt ttcttattag taatgcaaaa atggcgcata ttgtttttgt
    29281 tatgcagata catcaagcga aaatcccata actaattagg attattacaa aaaatcttca
    29341 ccaaattttt taaccgctgt ttgttgtaat atttttgcca tattcatagt aacgaattgg
    29401 aataagtacg acaacttagg gaataactcg atataaactc acctttgtaa ggggggggtt
    29461 aaatatatat taattcctgt gttcctgatt ttgcccggta atagagaatt attctcagag
    29521 aagctcatca tgattgcacc atacagatat catgatgata agaggatatt attcattgac
    29581 ttaaacaaaa tgacaaaatc catagaccca tagcctgggt actatatagg agtggtatgg
    29641 gcatcaaaca acacaatggg aataccaaag ccgatcgtct cgctgaatta aaaatccgtt
    29701 cgccctcaat tcaactgata aaatttggcg ctattggttt gaatgcaatt atcttttccc
    29761 ccctgctgat agctgctgat acaggaagtc aatatggcac caatattact attaatgatg
    29821 gtgacagaat tacaggagat accgccgatc catcaggaaa cctctatggt gtaatgaccc
    29881 cagcaggaaa cacgcctggc aatatcaacc tgggtaatga tgtcaccgtc aatgtcaacg
    29941 acgcctctgg atatgcaaaa ggaatcatta ttcagggcaa aaacagctcc ctgacagcta
    30001 accgactcac agtagatgtt gttggtcaaa cctctgccat cggcattaac ttaattggtg
    30061 actataccca tgctgactta ggcacaggca gcaccattaa gagtaacgat gacggcatca
    30121 ttattgggca tagctcaaca ctaacagcca ctcaattcac cattgaaaac tcgaacggta
    30181 taggcctaac catcaatgac tatggcacca gtgtcgatct tggaagcgga agtaaaatca
    30241 agaccgatgg aagtacaggt gtttatatcg gtggtctcaa cggcaataac gccaatggtg
    30301 ctgcgcgttt tacggcgaca gacctgacaa tcgatgttca gggctacagc gccatgggga
    30361 taaacgtaca gaaaaactct gttgtcgatc tcggaacaaa cagtaccatt aaaaccaatg
    30421 gcgataatgc tcacggcctc tggagctttg gccaggttag cgcgaatgca ctcactgttg
    30481 atgtaactgg agccgcggcc aatggcgtcg aagttcgtgg tggtacaacc actatcggtg
    30541 cagatagcca tatttcttcc gcgcagggcg gtggcctcgt caccagtagt tcagacgcga
    30601 caatcaattt ttctggcacg gcagcgcaac gaaacagcat cttttccggc ggttcttatg
    30661 gtgcctcggc ccagacggca acggctgtta tcaacatgca aaataccgat attacggttg
    30721 atcgtaatgg cagtctggcg ctgggtttgt gggcgctcag cggcggtaga ataaccggag
    30781 acagtttggc tatcaccggc gcggcaggag ccagagggat ttatgccatg accaacagcc
    30841 agatcgacct cacgagcgat ctggtcattg atatgagtac acccgaccag atggccatcg
    30901 caacgcaaca tgacgatggt tatgccgcca gccgcatcaa cgcctcgggt cgtatgctta
    30961 tcaacggtag cgttctttcc aaaggtgggc taatcaatct ggatatgcac cctgggtcgg
    31021 tttggacagg ttcctccctc agcgataatg tcaatggcgg gaaactggac gttgcaatga
    31081 ataacagcgt ctggaacgta acaagtaatt ctaatctcga cacgctggcg ctgagccatt
    31141 caactgtcga ttttgccagc cacgggtcaa ctgccggcac atttaccaca ttaaacgtag
    31201 agaacctgag cggtaacagt acctttatta tgcgtgctga tgttgttggc gagggtaatg
    31261 gcgttaataa tagaggggat ttattgaata tcagcgggag tagtgctggt aatcacgtat
    31321 tggctatccg caaccagggc agcgaggcca caacgggaaa tgaagttctg acagtggtaa
    31381 aaaccactga cggcgcggcc tcgttcagcg cgtcttctca ggttgagttg gggggatatc
    31441 tgtacgatgt gcgtaaaaat ggcactaact gggagcttta cgcttccggg acagttccgg
    31501 aaccgactcc taatcctgaa cccacaccag ctcccgctca gcctcccata gtcaaccccg
    31561 atcctacgcc tgaacccgct cccacgccta aacccaccac gaccgcagat gctggcggca
    31621 attatctcaa tgtcggttac ttattgaact atgttgaaaa ccgtacgctg atgcaacgga
    31681 tgggtgactt gcgaaatcag agtaaagacg gtaatatctg gttgcgcagt tatgggggaa
    31741 gcctggactc ctttgccagt ggcaaactga gcggctttga catgggttac agcggtatcc
    31801 agtttggtgg ggataaacgt ctctctgatg taatgccgtt gtatgtcggt ctgtatattg
    31861 actcaacaca tgcatcgccg gactatagcg gaggcgacgg taccgcacgt tcagactaca
    31921 tgggaatgta cgccagttac atggcacaaa acggttttta cagcgatctc gttataaaag
    31981 catcgcgcca gaaaaatagt ttccacgtac tggacagtca gaacaacggc gttaacgcca
    32041 acggcactgc gaatggaatg agcatctccc tggaagccgg gcagaggttc aacctgtccc
    32101 ctactggtta tgggttctat atagagccgc aaacccagct tacatacagc caccagaatg
    32161 agatggctat gaaggcgagt aatggcctca atatacatct gaatcactac gaatcgctgc
    32221 tggggcgtgc cagcatgata ctggggtatg acatcaccgc aggcaacagc cagctgaatg
    32281 tctatgtgaa gactggcgct atccgcgagt tttcagggga taccgaatat ctgttgaacg
    32341 actcccggga gaagtacagt ttcaaaggta atggctggaa taacggcgtg ggagtcagtg
    32401 cacagtataa caaacagcac acattctatc tcgaagcgga ttacacgcag ggtaacctct
    32461 ttgatcagaa gcaagtcaac ggaggatatc gcttcagctt ttaagtgcgg gatagacaag
    32521 cccttaaacg atgctgaaaa ttgttagcgt gcgccggaat gaaatacaga tcactctgat
    32581 gtatcagaaa tcgtttctgt ctttcatacc cggcgcttaa actgccctgc atcaatgcca
    32641 tgttgcttca ttttccgcca cagcgtagtg cggccgatcc caagtaaagc cgacatttcc
    32701 tgaatgcgac cgcctgtgac ctgggctgcg ttaataattg cctctttttc aacttccgca
    32761 aatgacagac tggtggaaag gcgggtggcg ctgacatcat ctgtcgcctg ctcggtaaac
    32821 agatgttcag gtaaatcact gacgcgaatg cgcccgttat cactactcag agcaagattc
    32881 tcgatgacgc tgtaaagttc aaaatcgttg cctggccatg cacaagaaac taggcgagcg
    32941 agggcatcgt catcaatttt cagccgcgta gagaagcgtt tttcaagact gcgtaatttg
    33001 ttattcacca gcgccggaat gctgccacgc cgcatacgca gaggcgggat ggtaatttca
    33061 aatgcatgca gcgcgtaata cagctggcga ctaaaacgat tttgttccac cagcattgcg
    33121 aggtccacgg tcgttgttgc aatcactttg acatcaattg gtattaaacg ccgcgcatcc
    33181 agtcgcgtga taaccccctg cttgataacc tgcagcaaag cagactgtaa ctccaccgcc
    33241 agatattcaa tcttttcaag aaacagcgtg ccgccgtgtg ccagttccag ccgactcaga
    33301 cggccatttt cattgtccgt gcgatcgcca ccaataaatt cttccgccag cgcagcatca
    33361 ccatataact cacaattgac ggcgatataa ggacctgcag cacgctcgct ttcattatga
    33421 attgcctgac ttagcagtgc cttgcccacg ccctcttctc cacaaagcag gacaggaaag
    33481 ctactgcgcg ccgcctggcg accaaaatga atcaagcggc gggtttgcgg atcgtcctgt
    33541 ggcatatgag cgaaggtatg gctgactttt cctaattgac tggtcattaa ctgccgcatc
    33601 tgttccacag gatggagcaa caaaataaag ctggttccct gcgtttctat tatcggttta
    33661 agggttatca ccgcatcaat aaactggtgc tggctttcaa aggttgcttc tacgtgtttg
    33721 agcggatgtg cctgttttat tgcttgttgc aatacggcgg gtaacgttaa gagttcagtg
    33781 attgcccgtc cctgacttgc cgtcgcgtca aggcgcaaga cccgcgccgc ctgggcatta
    33841 ataaattgca aattaccctg ctcgtcccag ctaatcacgc catcatccat actttctaac
    33901 agggcattaa gttgatttaa atgacggtta gtttcagcga gcaaactgtc cgtcagcagt
    33961 aaattaccga cctcgcgggc gattgccaac gtcaacggca aatcagctgc ggtagtttgc
    34021 tcaaccggac acgccagcgc tattgttccc gtcaatcggc ccttgctgtc aaacaacggc
    34081 gttgcacaaa aggcccagtt ccagagtgcc tgtttgaaat gttgatcggc catcgttttc
    34141 acggcctgac cagagatagc cgctaacgat agcgcacaag taccaataat tccctcggcg
    34201 caatacgtgc cgtcattgaa tcccagtgca cttagctgct gcaaggtttg cggatcgcca
    34261 ttacggctga gaatgcaggc ggtttcatcg aggataaaca gcgcacactc tcgcggtgcc
    34321 atatattccc aggcatcttc cagcgcagcc tgcccgagcg tcagcatcgc tttcttacgc
    34381 cgataaatag aagcaaatgt cacgccctgg gcctgatgtg gtacgttcca tgtctcccgt
    34441 ttcatcagct tattgcatcg ctcccaggag gttgcaatta agggagatat gccccgacca
    34501 tcgttgttaa aagcgccact catatcccgc atcccttatg acaaaaggtg ttccggctcg
    34561 caattttaag tattaataca cgtaaaagtt tcaatatgga acattcatta aagcatatgt
    34621 ttcactatga aacataattt gggctaattt tcttttccct tgcctgatgc acaatggatt
    34681 catcggcagt cagtggtcgc cgtgtcgttg aacatcatcc atgccctacc gtaattgctg
    34741 gagcaaaata atgaaaaaat tgatcaatga tgtgcaagac gtactggacg aacaactggc
    34801 aggactggcg aaagcgcatc catcgctgac actgcatcag gatccggtgt atgtcacccg
    34861 agctgatgcc cctgttgcag gaaaagtcgc cctgctgtcg ggtggcggca gcggacacga
    34921 gccgatgcac tgtggttata tcggtcaggg gatgctttcg ggggcctgtc cgggcgaaat
    34981 tttcacctca ccgacgcccg ataaaatctt tgaatgctcc atgcaaattg atggcggcga
    35041 aggtgtactg ttgattatca aaaattacac cggcgatatt cttaactttg aaacagcgac
    35101 cgagttactg cacgatagcg gcgtaaaagt gaccactgtg gtcattgatg acgacgttgc
    35161 ggtaaaagac agtctttata ccgccgggcg acgcggcgtt gccaacacag tattaattga
    35221 aaaactcgta ggcgcagcgg cggagcgtgg cgactcactg gaggcctgtg cggaactggg
    35281 gcgtaagttg aataatcaag gccactcaat aggtatcgct ctcggtgcct gtaccgttcc
    35341 tgccgcgggc aaaccttctt ttacccttgc ggataatgag atggagtttg gcgtcggcat
    35401 tcatggtgag ccaggtattg accgccgccc tttctcttcc cttgatcaaa ccgtcgatga
    35461 aatgttcgac accctgctgg aacatggctc ataccatcgc actttgcgtt tctgggatta
    35521 tcaacaaggc agctggcagg aagaaccaca aaccaaacaa ccgctccagt ctggcgatcg
    35581 ggtgattgca ctggttaaca atcttggcgc aactccgctt tctgagctgt acggcgtcta
    35641 taaccgcctg accacacgtt gccagcaagc gggattgact atcgaacgta atttaattgg
    35701 cgcgtactgc acctcactgg atatgaccgg tttctcaatc accttactga aagttgatga
    35761 cgaaacgctg gctctctggg acgccccggt ccacaccccg gcccttaact ggggtaaata
    35821 aggagaaagc aatgtcactg agcagaactc aaattgttaa ctggctcact cgttgtggcg
    35881 atattttcaa caccgagagc gagtatctta ccgggctgga tcgcgagatt ggcgacgctg
    35941 accacgggct aaatatgaac cgaggcttta gcaaagtggt ggaaaaactc cctgctatcg
    36001 cagataaaga tatcggtttc attctcaaaa ataccggtat gacgctgctt tccagcgtcg
    36061 gtggtgccag tggtccgctg ttcggtacct tctttatccg cgccgcacag gcgacccagg
    36121 cacggcaaag cctgacactg gaagagcttt atcagatgtt ccgcgatggc gcggacggcg
    36181 taatcagtcg cgggaaagcc gaacctggcg ataaaaccat gtgtgatgtg tgggtgccgg
    36241 tggtggaatc gttacgtcag tccagcgagc aaaatctctc tgttccggtg gcgctcgaag
    36301 ctgccagtag catcgccgaa tccgctgcac aaagtacgat tacgatgcaa gcccgcaaag
    36361 gccgcgccag ttatctcggt gaacgcagta ttggtcacca ggatcccggc gcgacctcgg
    36421 tgatgtttat gatgcaaatg ttggcgttag ccgcaaaaga gtaaggaatt ggtgatggta
    36481 aacctggtca tagtttcaca tagcagccga ctgggagaag gtgtcggtga attagcccgt
    36541 cagatgttaa tgagtgatag ttgtaaaatc gccattgccg cgggaattga cgatccacaa
    36601 aatcccattg gtaccgatgc cgtcaaagtg atggaggcca tcgaatctgt tgctgatgcc
    36661 gaccatgtgc tggtcatgat ggatatgggt agcgcattat tgagtgctga aactgcgctg
    36721 gaattgctgg ctcccgagat cgccgcaaaa gtacgtttgt gtgctgcgcc gttggtcgaa
    36781 ggtacactgg cagcaacggt cagcgcggcc tcgggggcgg atatcgacaa agttatcttt
    36841 gacgctatgc atgcgctgga agccaaacgt gaacaactgg gtttaccgtc ctccgacact
    36901 gaaatctctg acacatgtcc tccgtacgat gaagaagccc gttctctggc ggtggtcata
    36961 aaaaaccgta acggcctgca tgtacgtccg gcctcccggc tggtttatac cttatcgaca
    37021 tttaatgccg atatgttgct ggaaaaaaac ggcaaatgcg tcacaccaga gagtattaac
    37081 cagattgcgt tactacaagt tcgctataac gatacgctgc gcctgattgc gaaagggcca
    37141 gaagctgaag aggcactgat cgctttccgt cacctggctg aagataactt tggtgaaacg
    37201 gaggaagtcg ctccacctac tctgcgtccc gttctgcctg tttcgggtaa agccttttat
    37261 tatcaaccag ttttatgtac ggtacaggca aaatcaaccc tgaccgcgga agaagaacaa
    37321 gatcgattac gccaggctat tgacttcacg ttattagatc tgatgacgtt aacagcgaaa
    37381 gcagaagcca gcgggcttga cgatattgcc gcaatctttt ctggtcacca tacactgtta
    37441 ggtgatccgg aactgctggc ggcggcaagc gaactccttc agcatgaaca ttgcacggca
    37501 gaatatgcct ggcagcaagt tcttaaagaa cttagccagc aataccagca actggatgat
    37561 gaatatctac aagctcgcta tattgatgtg gacgatcttc tgcatcgcac cctggtccac
    37621 ctgacccaaa cgaaagaaga actcccgcag tttaactcgc caactattct actggcggag
    37681 aacatttatc cttccacagt actgcaactg gatccggcgg ttgtaaaagg tatctgcctt
    37741 agcgccggaa gtccggtatc ccacagcgcc ctaatcgccc gtgaactggg gattggctgg
    37801 atttgccagc agggtgagaa actgtatgcg atacaaccag aagaaacgct aacgctggac
    37861 gttaaaacgc aacgtttcaa ccgtcagggt taagccaccg ttcggatggc atcgttctga
    37921 tgtcatccgg caatttaccc agcactgatg ttttttaact acctgttata ttttttacca
    37981 atttatgatg atttttcttc gctaatcgcg aaccaaatgt cgctggctgg ctaataaaca
    38041 gacgttattt gatcactcta tttcgccgtt atttatccct ttaattccct tttctaaaat
    38101 gcctgaccgt tcgcagaatg agatttcgat catgcagcta gtgcgatcct gaactaaggt
    38161 tttctgatac ttgaataccg ttttattcgg tttcgccaaa ggagaatgat tgatgaaatc
    38221 ccccgcacct tctcgcccgc aaaaaatggc gttaattcca gcctgtatct ttttgtgttt
    38281 cgctgcgcta tcggtgcagg cagaagaaac accggtaaca ccacagccgc ctgatatttt
    38341 attagggccg ctgtttaatg atgtgcaaaa cgccaaactt tttccggacc aaaaaacctt
    38401 tgccgatgcc gtgccgaaca gcgatccgct gatgatcctt gctgattatc ggatgcagca
    38461 aaaccagagc ggatttgatc tgcgccattt cgttaacgtc aatttcaccc tgccgaaaga
    38521 aggcgagaaa tatgttccgc cagaggggca gtcactgcgc gaacatattg acggactttg
    38581 gccggtatta acgcgttcta ccgaaaacac cgaaaaatgg gattctctgt taccgctgcc
    38641 ggaaccttat gtcgtgccgg gcggacgttt tcgcgaggta tattactggg acagttactt
    38701 caccatgtta ggacttgccg aaagtggtca ctgggataaa gtcgcggata tggtggccaa
    38761 ttttgctcat gaaataaaca cttacggtca tattcccaac ggcaaccgca gttactattt
    38821 aagccgctcg caaccgccct tctttgccct gatggtagag ttactggcgc agcatgaagg
    38881 cgatgccgcg ttgaagcaat acctgccgca aatgcaaaaa gaatatgctt actggatgga
    38941 cggtgttgaa aacctgcaag ccggacaaca ggaaaaacgc gttgtaaaac ttcaggatgg
    39001 tacccttctc aaccgctact gggacgatcg cgatacgcca cgaccagagt catgggtgga
    39061 agatattgcc accgccaaaa gcaatccgaa tcgacctgcc actgaaattt accgcgacct
    39121 gcgctctgcc gctgcgtctg gctgggattt cagctcgcgc tggatggaca acccgcagca
    39181 gttaaatacc ttacgcacca ccagcatcgt accggtcgat ctgaacagcc tgatgtttaa
    39241 aatggaaaaa atcctcgccc gcgccagcaa agctgcagga gataacgcga tggcaaacca
    39301 gtacgaaacg ctggcgaatg cccgtcaaaa agggatcgaa aaatacctgt ggaacgatca
    39361 acaaggctgg tatgccgatt acgacctgaa aagtcataaa gtgcgcaatc agttaaccgc
    39421 ggccgccctg ttcccgctgt acgtcaatgc ggcagcgaaa gatcgcgcca acaaaatggc
    39481 gacggcgacg aaaacacatc tgctgcaacc cggcggcctg aacaccacgt cggtgaaaag
    39541 tgggcaacaa tgggatgcgc caaatggctg ggcaccgtta cagtgggtcg cgacagaagg
    39601 attacaaaac tacgggcaaa aagaggtggc gatggacatt agctggcact tcctgaccaa
    39661 tgttcagcac acctatgacc gggagaaaaa gctggtggaa aaatatgatg tcagcaccac
    39721 cggaacgggg ggcggcggtg gcgaatatcc attacaggat ggctttggct ggaccaatgg
    39781 cgtgacgctg aaaatgctgg atttgatctg cccgaaagag caaccgtgtg acaatgttcc
    39841 ggcgacgcat ccgaccgtta agtcagcaac gacgcaaccc tcaaccaaag aggcacaacc
    39901 cacaccttaa ccagcgctta ctccgacaac gtcattcggc ttctccggat ggcgttgtcg
    39961 gcagataaca ccatatctat ggttttttct acacagacgg tttttcaagt cctggcggga
    40021 agtattgcgc aaatgtttgc catacgtggt tgccgaagat agcgtcattt ctacgggtaa
    40081 gtgtttcact ttcatcaccc ggcgatttac gccgacgttg gctatataaa gcatcaattg
    40141 ctaccgaata ttgttacggt gttcactcat caacgttttt ttgctttctg gagttaaatt
    40201 aataatcaca taatccagac actccagaga atttagcaac caaaaaaaga attcagacca
    40261 ttcctcttcg cgtaactgaa gcattgccga acgggtattt tggcttaatc tgccagaagg
    40321 attgatcaat tctaaatgtt caataagcgc agccaaaacc gcctcactat ccgccccatt
    40381 tttaacgcct gactggctac tgtctaaaat atttattttc tgaatcacac caaaaacaac
    40441 gaaacaataa ttaaaaaagg attctgttgt tgttaccggg aaccgactta tttcgctcag
    40501 aaagtcgttc aaccagctcc gcagtgataa gattcgctga tgatacagtg tatcctcact
    40561 gcgtgctgga ggtgccagta taatatgttg atcaatacag tatttattgt gttcagtcgt
    40621 ttcctgatgc gtttggttct atgcggattg tcgctggcga aataaatggc ttgcggtaaa
    40681 tgccgttaca gagcagcgca acgcgtcaat tccttcggag tgcccggctt ttggcgagag
    40741 ttccggtttt atgcgtccag gtagcggaaa tttcacccga atgagaaatg cattaataaa
    40801 atgg
//
LOCUS       DAJTQY010000026        39814 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.026, whole genome shotgun sequence.
ACCESSION   DAJTQY010000026 DAJTQY010000000
VERSION     DAJTQY010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39814
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.026"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            64..225
                     /locus_tag="OO349_001452"
                     /pseudo
     CDS             64..225
                     /locus_tag="OO349_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            454..1044
                     /gene="uidR"
                     /locus_tag="OO349_001453"
     CDS             454..1044
                     /gene="uidR"
                     /locus_tag="OO349_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HCR5866583.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            1432..3105
                     /gene="uidA"
                     /locus_tag="OO349_001454"
     CDS             1432..3105
                     /gene="uidA"
                     /locus_tag="OO349_001454"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HCR5866584.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWCESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDISVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATS"
     gene            3141..4166
                     /gene="uidB"
                     /locus_tag="OO349_001455"
                     /pseudo
     CDS             3141..4166
                     /gene="uidB"
                     /locus_tag="OO349_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002431055.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
     gene            4205..5470
                     /gene="uidC"
                     /locus_tag="OO349_001456"
     CDS             4205..5470
                     /gene="uidC"
                     /locus_tag="OO349_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HCR5866585.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIVGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMVGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGAN
                     YRVTPQFTLTFAPIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(5696..7204)
                     /locus_tag="OO349_001457"
     CDS             complement(5696..7204)
                     /locus_tag="OO349_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HCR5866586.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLN
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLGSFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMSLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(7305..8480)
                     /gene="manA"
                     /locus_tag="OO349_001458"
     CDS             complement(7305..8480)
                     /gene="manA"
                     /locus_tag="OO349_001458"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HCR5866587.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     HNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPHAERLSELFANLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            8679..10325
                     /gene="fumB"
                     /locus_tag="OO349_001459"
     CDS             8679..10325
                     /gene="fumB"
                     /locus_tag="OO349_001459"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCR5866588.1"
                     /translation="MSNKPFHYQSPFPLKKNDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            10468..11871
                     /gene="fumC"
                     /locus_tag="OO349_001460"
     CDS             10468..11871
                     /gene="fumC"
                     /locus_tag="OO349_001460"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HCR5866589.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLDLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(11868..12797)
                     /gene="tus"
                     /locus_tag="OO349_001461"
     CDS             complement(11868..12797)
                     /gene="tus"
                     /locus_tag="OO349_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HCR5866590.1"
                     /translation="MARYDLVDRLNTTFRQMEQELATFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(12873..14174)
                     /gene="rstB"
                     /locus_tag="OO349_001462"
     CDS             complement(12873..14174)
                     /gene="rstB"
                     /locus_tag="OO349_001462"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HCR5866591.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLMQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDISELGGARFSFSWPLWHNIPQFTSA"
     gene            complement(14353..15672)
                     /locus_tag="OO349_001463"
     CDS             complement(14353..15672)
                     /locus_tag="OO349_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HCR5866592.1"
                     /translation="MLFLICSWGIFKTDQKSESLSKSVFIAIFLGAIIGISLHFIPET
                     SSETVIEWYNIAGNGYVNLLKLVAVPLIFISILSAINKLENSAGIGKMSLTIVACMLC
                     LVMIAGLVGLAIASAIGLDASAFGHMQSSLTSDDVSKTAAVSLPRLITSLIPTNLFLD
                     LTGARSVSVIGIVIFTILAGIALLKVKKDAPAEGEKLSAGINAIQIWVMKMVRIVIAL
                     TPYGVMALMAKVFSTYQLSQFTNLLGFIVACYLAVFVMFIVHSGILLLIGKNPVNYYK
                     TVWQVLTFAFVSRSSAASIPLAISALEKLGVPNTIANIAASFGSSMGQNGCAGIYPAV
                     MVAMIAPTMGIDPFSLQFLATLLPAIALGSIGVAGVGSGGTFAALIVLSTLNFPVALV
                     VIFIAIEPIVDMARTALNVNGSIMSGVISARLLKDRETKEQQSVTLN"
     gene            complement(15897..16640)
                     /gene="rstA"
                     /locus_tag="OO349_001464"
     CDS             complement(15897..16640)
                     /gene="rstA"
                     /locus_tag="OO349_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HCR5866593.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWDSDNKQSDD"
     gene            16769..17104
                     /locus_tag="OO349_001465"
     CDS             16769..17104
                     /locus_tag="OO349_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HCR5866594.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            complement(17101..17823)
                     /gene="folM"
                     /locus_tag="OO349_001466"
     CDS             complement(17101..17823)
                     /gene="folM"
                     /locus_tag="OO349_001466"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HCR5866595.1"
                     /translation="MGKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKTTHGLRAILHNASAWIAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(17860..19242)
                     /locus_tag="OO349_001467"
     CDS             complement(17860..19242)
                     /locus_tag="OO349_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5866596.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            complement(19428..20372)
                     /locus_tag="OO349_001468"
     CDS             complement(19428..20372)
                     /locus_tag="OO349_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR5866597.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQKRGNNLTVSADLYK"
     gene            20896..22428
                     /gene="pntA"
                     /locus_tag="OO349_001469"
     CDS             20896..22428
                     /gene="pntA"
                     /locus_tag="OO349_001469"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HCR5866598.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYIVPGEIFSTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLSSVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            22439..23827
                     /gene="pntB"
                     /locus_tag="OO349_001470"
     CDS             22439..23827
                     /gene="pntB"
                     /locus_tag="OO349_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HCR5866599.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(23852..24886)
                     /gene="tqsA"
                     /locus_tag="OO349_001471"
     CDS             complement(23852..24886)
                     /gene="tqsA"
                     /locus_tag="OO349_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HCR5866600.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTWTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            25297..25662
                     /gene="mdtJ"
                     /locus_tag="OO349_001472"
     CDS             25297..25662
                     /gene="mdtJ"
                     /locus_tag="OO349_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HCR5866601.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            25649..25978
                     /gene="mdtI"
                     /locus_tag="OO349_001473"
     CDS             25649..25978
                     /gene="mdtI"
                     /locus_tag="OO349_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HCR5866602.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVVYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(26017..26838)
                     /locus_tag="OO349_001474"
     CDS             complement(26017..26838)
                     /locus_tag="OO349_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HCR5866603.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(26938..27021)
                     /locus_tag="OO349_001475"
     CDS             complement(26938..27021)
                     /locus_tag="OO349_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5866604.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            complement(27114..27449)
                     /locus_tag="OO349_001476"
     CDS             complement(27114..27449)
                     /locus_tag="OO349_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HCR5866605.1"
                     /translation="MKKQIEGMTMKKVLALVVAAAMGLSSAAFAAETATTPAPTATTT
                     KAAPAKTTHHKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHGHQQP
                     AKPAAQPAA"
     gene            complement(28017..28274)
                     /locus_tag="OO349_001477"
     CDS             complement(28017..28274)
                     /locus_tag="OO349_001477"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5866606.1"
                     /translation="MVGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMPFGLL
                     MTLFSSLWLIFAGMLLFSAGFFAAHSVASSWIGPRAKRAKG"
     gene            complement(28290..29099)
                     /locus_tag="OO349_001478"
     CDS             complement(28290..29099)
                     /locus_tag="OO349_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5866607.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFC
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSP"
     gene            29206..30108
                     /locus_tag="OO349_001479"
     CDS             29206..30108
                     /locus_tag="OO349_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5866608.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPYDHPLANNPSVTLAELAELAKEPFVFFDPHVGTGLYDDILGLMRR
                     YHLTPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWL
                     VWPKHHEQSPAARNFRIHLLNALR"
     gene            30243..31463
                     /gene="mlc"
                     /locus_tag="OO349_001480"
                     /gene_synonym="dgsA"
     CDS             30243..31463
                     /gene="mlc"
                     /locus_tag="OO349_001480"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HCR5866609.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVRELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGVGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIIIGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            31588..32283
                     /gene="bioD"
                     /locus_tag="OO349_001481"
     CDS             31588..32283
                     /gene="bioD"
                     /locus_tag="OO349_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCR5866610.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKRVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(32236..33528)
                     /gene="clcB"
                     /locus_tag="OO349_001482"
     CDS             complement(32236..33528)
                     /gene="clcB"
                     /locus_tag="OO349_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HCR5866611.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAVFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSSWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAIVAWLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALVG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(33687..34301)
                     /gene="dmsD"
                     /locus_tag="OO349_001483"
     CDS             complement(33687..34301)
                     /gene="dmsD"
                     /locus_tag="OO349_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HCR5866612.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(34344..35198)
                     /locus_tag="OO349_001484"
     CDS             complement(34344..35198)
                     /locus_tag="OO349_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HCR5866613.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQHIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAEVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILVGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(35200..35817)
                     /gene="dmsB"
                     /locus_tag="OO349_001485"
     CDS             complement(35200..35817)
                     /gene="dmsB"
                     /locus_tag="OO349_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HCR5866614.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGIWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            complement(35828..37866)
                     /locus_tag="OO349_001486"
                     /pseudo
     CDS             complement(35828..37866)
                     /locus_tag="OO349_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            complement(37927..39036)
                     /locus_tag="OO349_001487"
     CDS             complement(37927..39036)
                     /locus_tag="OO349_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HCR5866615.1"
                     /translation="MVNVSHIRTLSSVESTTSSPSIRLTVESIWNYAGNTLVNQHSDI
                     NKTHEILQDESKCEMIVVIENFMTSSAKYADILLADLMTVEQEDIIPNDYAGNMGYLI
                     FLQPVTSEKFERKPIYWILSEVAKRLGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPAL
                     PSYDELKKMGIYKRKDPNGHFVAYKAFRDDPEANPLKTPSGKIEIYSSKLAEIARTWE
                     LEKDEVISPLPVYASTFEGWDSPERRTFPLQLFGFHYKSRTHSTYGNIDVLKAACRQE
                     VWINPIDAQKRGIANGDMVRVFNHRGEVRLPAKVTPRILPGVSAMGQGAWHEPNMSGD
                     KIDHGGCVNTLTTLRPSPLAKGNPQHTNLVEIEKI"
     gene            39019..>39814
                     /locus_tag="OO349_001488"
     CDS             39019..>39814
                     /locus_tag="OO349_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001429194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR5866616.1"
                     /translation="MTDINHDDTAYPSSSFSGSPYLYQQVYTSSPDTRLTQIKDLATP
                     QLVRRTNGRYTVRFRLKGATSPFLSVSTRSTDRRTATMRQRELAATAKAFLLDNPEVT
                     LQELTAHIRSMAEQFLTDASDDYWNGVEVATLVDEKSNLRELAATRALSVDQQRGIKL
                     ALEVLTAAQQRVDTGDTSGLIKLIDGGVSQLTDDNNHTDYSTIGDSTSILKNEQGDRP
                     AVFTQERQLLTPESRESSVLFQELVTSLLAEKVQTLKQSSFKDLSAS"
BASE COUNT        10144 a         9649 c        10187 g         9834 t
ORIGIN      
        1 gcatgacaag gtcatcgggc attatctgaa cataaaacac tatcagtaag ttggagtcat
       61 tacccagaaa ttgaacaaaa aatgttacag cacacgccga tcagacgtct gggccaaccg
      121 caagatattg ctaacgcggc gctgttcctt tgctcgcctg cagccagctg ggtaagcgga
      181 caaattctca ccgtctccgg tggtggggta caggagctca attaatacac taacgaaccg
      241 gtaaacggtc gtatgtgttg tttaccggga tagcgtgtgc aattcatcaa cgtctctgct
      301 aaacaactgg cagccaaatc acggctattg gttaaccaat tgctgggtga aaagtatacg
      361 aatagagtgt accttcgcac tattcaacag caatgatagg cgctcacctg acaacgcggt
      421 aaactagtta ttcacgctaa ctataatggt ttaatgatgg ataacatgca gactgaagca
      481 caaccgacac ggacccggat cctcaatgct gccagagaga ttttttcaga aaatggattt
      541 cacagtgcct cgatgaaagc catctgtaaa tcttgcgcca ttagtcctgg gacgctctat
      601 caccatttca tctccaaaga agccttgatt caggcgatta tcttacagga ccaggagagg
      661 gcgctggctc gtttccggga accgattgaa gggattcatt ttgttgacta tatggtcgag
      721 tctattgtct ctctcaccca tgaagccttt gggcaacggg cgctggtggt cgaaattatg
      781 gcggaaggga tgcgtaaccc acaggtcgcc gccatgctta aaaataagca tatgacgatc
      841 actgaatttg ttgcccagcg gatgcgtgat gcccagcaaa aaggcgagat aagcccagac
      901 atcaacacgg caatgacttc acgtttactg ctggatctga cctacggtgt actggccgat
      961 atcgaagcgg aagacctggc gcgtgaagcg tcgtttgctc agggattacg cgcgatgatt
     1021 ggcggtatat taaccgcatc ctgatcctct catttcgcgg gctggtgata actgtgcccg
     1081 cgtttcatat cgtaatttct ctgtgcaaaa attatcctcc ccgtgttcgg agaattcccc
     1141 cccaaaatat tcaccgtagc catatgtcat gagagtttat cgttcccaat actctcgaac
     1201 gaacgttcga ttgcttattt tatggcttct gtcaacgctg ttttaaagat taatgcgatc
     1261 catatcacgc tgtgggtatt gcagtttttg gttttttgat cgcggtgtca gttcttttta
     1321 tttccatttc tcttccatgg gtttctcaca gataactgtg tgcaacacag aattggttaa
     1381 ctaatcagat taaaggttga ccagtatttt tatcttaatg aggagtccct tatgttacgt
     1441 cctgtagaaa ccccaacccg tgaaatcaaa aaactcgacg gcctgtgggc attcagtctg
     1501 gatcgcgaaa actgtggaat tgatcagcgt tggtgcgaaa gcgcgttaca agaaagccgg
     1561 gcaattgcag tgccaggcag ttttaacgat cagttcgccg atgcagatat tcgtaattat
     1621 gtgggcaacg tctggtatca gcgcgaagtc tttataccga aaggttgggc aggccagcgt
     1681 atcgtgctgc gtttcgatgc ggtcactcat tacggcaaag tgtgggtcaa taatcaggaa
     1741 gtgatggagc atcagggcgg ctatacgcca tttgaagccg atgtcacgcc gtatgttatt
     1801 gccgggaaaa gtgtacgtat caccgtttgt gtgaacaacg aactgaactg gcagactatc
     1861 ccgccgggaa tggtgattac cgacgaaaac ggcaagaaaa agcagtctta cttccatgat
     1921 ttctttaact atgccgggat ccatcgcagc gtaatgctct acaccacgcc gaacacctgg
     1981 gtggacgata tcaccgtggt gacgcatgtc gcgcaagact gtaaccacgc gtctgttgac
     2041 tggcaggtgg tggccaatgg tgatatcagc gttgaactgc gtgatgcgga tcaacaggtg
     2101 gttgcaactg gacaaggcac cagcgggact ttgcaagtgg tgaatccgca cctctggcaa
     2161 ccgggtgaag gttatctcta tgaactgtgc gtcacagcca aaagccagac agagtgtgat
     2221 atctacccgc tgcgcgtcgg catccggtca gtggcagtga agggcgaaca gttcctgatt
     2281 aaccacaaac cgttctactt tactggcttt ggtcgtcatg aagatgcgga tttgcgaggc
     2341 aaaggattcg ataacgtgct gatggtgcac gatcacgcat taatggactg gattggggcc
     2401 aactcctacc gtacctcgca ttacccttac gctgaagaga tgctcgactg ggcagatgaa
     2461 catggcatcg tggtgattga tgaaactgca gctgtcggct ttaacctctc tttaggcatt
     2521 ggtttcgaag cgggcaacaa gccgaaagaa ctgtacagcg aagaggctgt caacggggaa
     2581 actcagcagg cgcacttaca ggcgattaaa gagctgatag cgcgtgacaa aaaccaccca
     2641 agcgtggtga tgtggagtat tgccaacgaa ccggataccc gtccgcaagg tgcacgggaa
     2701 tatttcgcgc cactggcgga agcaacgcgt aaactcgacc cgacgcgtcc gatcacctgc
     2761 gtcaatgtaa tgttctgcga cgctcacacc gataccatca gcgatctctt tgatgtgctg
     2821 tgcctgaacc gttattacgg ttggtatgtc caaagcggcg atttggaaac ggcagagaag
     2881 gtactggaaa aagaactgct ggcctggcag gagaaactgc atcagccgat tatcatcacc
     2941 gaatacggcg tggatacgtt agccggcctg cactcaatgt acaccgacat gtggagtgaa
     3001 gagtatcagt gtgcatggct ggatatgtat caccgcgtct ttgatcgcgt cagcgccgtc
     3061 gtcggtgaac aggtatggaa tttcgccgat tttgcgacct cgtaaggcat attgcgcgtt
     3121 ggcggtaaca attgacctac atgggcctcg ggctttgcta cagcctggtg aatattcctt
     3181 atggttcact tgctaccgcg atgacccaac aaccacaatc tcgcgcccgt ctgggcgcgg
     3241 ctcgtgggat tgccgcttca ttgacctttg tatgcctggc atttctgata ggaccgagca
     3301 ttaagaactc cagcccggaa gagatggtgt cggtatacca tttctggacg attgtgctgg
     3361 tgattgccgg aatggtgctt tacttcatct gcttcaaatc gacgcgtgaa aatgtggtac
     3421 gtatcgtggc gcagccgtca ttgaagatca gtttgcaaac cctgaaacgg aatcgcccgc
     3481 tgtttatgtt gtgcatcggt gcgctgtgtg tgctgatttc gaccttcgcg gtcagcgcct
     3541 cgtcgttgtt ctacgtgcgc tatgtgttaa atgataccgg gctgttcact gtgctggtac
     3601 tggtgcaaaa cctggttggt actgtggcat cggcaccgct tgtgccgggg atggtcgcga
     3661 ggatcggtaa aaagaatacc ttcctgattg gcgctttgct gggaacctgc ggttatctgc
     3721 tattcttctg ggtttccgtc tggtcactgc cggtggcgtt ggttgcgttg gccatcgctt
     3781 caattggtca gggcgttact atgactgtga tgtgggcact ggaagctgat accgtagaat
     3841 acggtgaata cctgaccggc gtgcgaattg aagggctcac ctattcacta ttctcattta
     3901 cccgtaaatg cggtcaggca atcggaggtt caattcctgc ctttattttg ggattaagcg
     3961 gatatatcgc caatcaggtg taaacgccgg aagttattat gggcatccgc acatcaattg
     4021 ccttagtacc ttgcggattt atgctactgg cattcgttat tatctggttt tatccgctca
     4081 cggataaaaa attcaaagaa atcgtggttg aaattgataa tcgtaaaaaa gtgcagcagc
     4141 aattaatcag cgatatcact aattaatatt caataaaaat aatcagaaca tcaaaggtgc
     4201 aactatgaga aaaatagtgg ccatggccgt tatttgcctg acggctgcct ctggccttac
     4261 ctctgcttac gcggcgcaac tggctgacga tgaagcggga ctacgcatca gactgaaaaa
     4321 cgaattgcgc agggctgata agcccagtgc tggcgcggga agagatattt acgcatgggt
     4381 acagggagga ttgctcgatt tcaatagtgg ttattattcc aatattgttg gcgttgaagg
     4441 cggagcgtat tatgtttata aattaggtgc tcgtgctgat atgagtaccc ggtggtatct
     4501 tgatggtgat aaaagttttg gctttgccct gggggcagta aaaataaaac ccagtgaaaa
     4561 tagcctgctt aaattaggtc gcttcgggac ggattatagt tatggtagct taccttatcg
     4621 tattccgtta atggttggca gttcgcaacg tacattaccg acagtttctg aaggagcatt
     4681 aggttattgg gctttaacgc caaatattga tctgtgggga atgtggcgtt cacgagtatt
     4741 tttatggact gattcaacaa ccggtattcg tgatgaaggg gtgtataaca gccagacggg
     4801 aaaatacgat aaacatcgcg cacgttcttt tttagccgcc agttggcatg atgataccag
     4861 tcgctattct ctgggggcat cggtacagaa agatgtttcc aatcagatac aaagtattct
     4921 cgagaaaagc ataccgctcg acccgaatta tacgttgaaa ggggagttgc tcggctttta
     4981 cgcgcagctc gaaggtttaa gtcgtaatac cagccagccc aatgaaacgg cgttggttag
     5041 tggacaattg acctggaatg cgccgtgggg aagcgtattt ggcagtggtg gttatttgcg
     5101 ccatgcaatg aatggtgccg tggtggatac cgacattggc tatccctttt cattaagtct
     5161 tgatcgtaac cgtgaaggaa tgcagtcctg gcaattaggt gccaactacc gtgtaactcc
     5221 gcaatttacg ctgacatttg caccgattgt gactcgcggc tatgaatcca gtaaacgaga
     5281 tgtgcggatt gaaggcgcgg gtatcttagg tggtatgaac tatcgggtca gcgaagggcc
     5341 gttacaaggg atgaatttct ttcttgctgc cgataaaggg cgggaaaagc gcgatggcag
     5401 tacgctgggc gatcgcctga attactggga tgtgaaaatg agtattcagt atgactttat
     5461 gctgaagtaa gaaataacgc cggagagaaa aacctccggc gtttcagatt gttgacaaag
     5521 tgcgcgtttt ttatgccgga tgcggcgtaa acgccttatc cagcctacaa aaactcataa
     5581 attcaaagtg ttgcaggaaa aggtaggcct gataagcgta gcgcatcagg caatctctgg
     5641 tttgttttca gatgaaaacg ccggagtgaa aattctccgg cgttttggcc gtgaattact
     5701 gctgcggaat tgccggtaca gccggaacgt taagagctgg catcgcaaac atgccaacaa
     5761 aatcttctaa cgacattttc tgcccattta acgttatctg accgttagca tattgcaggc
     5821 tggtggtgat ggtattgtcc tgcaaggtgg tcagacggaa catctgcccc attgctgatg
     5881 caccttcaac ttgctgtttc gccagttttt tcgcttgatc ttcctgataa ccttccagct
     5941 tcgctacctg agtcatcaac tcagttgcca tatccaccgg aatggtcagt ttcgcatcca
     6001 gagatttaac cgaacgatct acttcctgcg ccagcgtttg cggcgcttct ttagtcgttg
     6061 ccggatcttt caggaacagc gacagattca gcgcactttc accctgactg tttttccagc
     6121 ttagcggcgc gatagtaatc accggatcgc ctttcaacat cagcggcagg gcgctaaaga
     6181 aggcttccgt cactttctcc tgataaagtt cgggattgtt ggcaatttct ggctgtgcca
     6241 gcagcgcctg agtttgcgcg ttatattgct ggctaaactg atgccaggct tcaccatcaa
     6301 tctggccgac ttttaaagtc agcttgccgc tgcccagatc ctgattctgt accttcaggc
     6361 tgtttagcga gtaatccagt tggctattga tcgttttacc gtcattgacc agatccgatt
     6421 taccgctgat ctccatgcct tccagcagtg ccagttcttt gccttccact gaaatggtca
     6481 ttttttccag tgacagtttt tggtttccta cgcgctcacc aaaacttccc agcgtgctgg
     6541 aaccgtcggt tttcagatta ttaaaggtca actgcacttt ctggttgtat tcgttaactg
     6601 cgtctatccg accactttgc gcctctccgg aaagggagat ggctttgccg tctctgtcag
     6661 catttaactg gaactcgccg ccgctaaagg cgactttttc atccttttgc tcgtaattca
     6721 gtggattgag cgaaatatcg gaactggaat caccgctgta accaatgcgc gagttaatct
     6781 caaaaggcgt ttcacctttt gccatatcaa acagtggttt gcttacttcg ttattaacca
     6841 gcgtggtttg aattgatgcc atcgacggga tcaggttcag ttttttaagc tgggcaagcg
     6901 ggaagggacc atgatcaacc gattcgttga agatgacgct ctgaccgctt ttaatccacg
     6961 gattttcttt cccggcaatg ggtttcacca acagttgcaa ctggctgctg aatacgccgc
     7021 gatgatagtt ttgataactc acttccaggt tggattcagg agctgtcagt ttgagttgcg
     7081 cgttcgcctg cgcgaccatg tcttcgagat gggtttcaat cttcttgcct gtataccatg
     7141 cgccgcctgt ccagactacg cctagcgcaa caatgacgcc taccgctacc agcgatttat
     7201 tcataatgat tatccataaa atgaaatcag gcggactggc cgcctgaagg tgttataagc
     7261 ctttaataag cttagcaaga gatgttaatt ttttcagtaa gctcttacag cttgttgtaa
     7321 acacgcgcta aacggccgtg gcctttgaca gtcaccggtg attcgttggc ggcaataaac
     7381 gctgattcac ccggtttaag ctgtaactgc tgagaacctt tccacaacgt tgcatcgcct
     7441 tcgacgcaga acaaaatggc ggcactctgc tggctgatgg tggtttcttt atcactaagg
     7501 tcatgcagcg agaaggcaaa atcatccaca ggaatcggga agtccagttc tgcaccttgt
     7561 ttcaccggct gggtcaacaa ctggttagcc ggtttggctt cgaatttcac attggcaacc
     7621 agttccggaa tatcaatgta tttaggcgtc agacctgcac gcagcacgtt atcggagttc
     7681 gccatcactt ccagcgccac gccttgcagg taagcgtgcg gtgtttcagc gaacaggaac
     7741 atcgcttcgc caggattcaa tttcaccaca ttcagtaaca gcggggagaa caggccgctg
     7801 tcttccgggt aaaattcaga aattaaacga atcgtttgcc acggttcacc ctgctgactg
     7861 tcgagcgccg attttaaaat cgccagcgcg cgggattttt cttcaccctg catattcaac
     7921 aggttggcga acagttcgct taaacgttcg gcatgtggct gttgtaaaaa gtgagcaatc
     7981 gccggatgtg caccggcgac cggctggagt agggagacaa tctcggaaaa ttcacgaaac
     8041 gcgttcatcg caaggaaagg cgtcagcgca aaaaccagct ccggcttgtg gttaggatct
     8101 ttatagttac gctcggcggc atccatcggg atacctgcgg cattttcttt ggcaaaaccg
     8161 atttcagaat tgtgtttgtt tggatgaacc tgaatggaga gtggctgtgc tgcgcataat
     8221 actttgaaca ggaaaggcag ttcgccaaag cgtttggcaa cggcctctcc gagcagagtc
     8281 gatttatcac tctcaatcac atcacgcagt gaaacgatat ctccggcggc attctgcact
     8341 cgtgaactgc ttttcggatg tgcgcccatc cacagctcgg ccatcggctg gctggacgga
     8401 ttttccatac cataaagttc agtcaacgcc gttttgctac cccaggcata gttttgcact
     8461 gagttaatga gtttttgcat gatcaatccc tgttttaatg tggaaattaa tcccgctatt
     8521 aaagcaagaa tcctacggga agtaacctgg agccgcaaaa agtcgtacta gtctcagttt
     8581 ttgttaaaaa actgtgtagg atattgttac tcgcttttaa cagggcaacg gaacacccgc
     8641 ccagagcata accaaaccag gcagtaagtg agagaacaat gtcaaacaaa ccctttcatt
     8701 atcagtctcc ttttccactc aaaaaaaatg atactgagta ttacctgcta accagtgaac
     8761 acgttagcgt atctgaattt gaagggcagg agattttgaa agtcgcaccc gaagcgttaa
     8821 ctctgttggc gcgtcaggcg tttcatgatg cgtcgtttat gctgcgtccg gctcaccaac
     8881 aacaggtggc cgacattctg cgtgacccgg aggccagcga aaatgataaa tatgtggcgc
     8941 tgcaattcct gcgtaactcc gacatcgcgg cgaaaggcgt tctgccaacc tgtcaggata
     9001 ccggcaccgc gattattgtt ggtaaaaaag ggcagcgtgt atggaccggt ggtggtgatg
     9061 aagcggcgct ggcgcgcggt gtctataaca cttatatcga agataatctg cgctactcgc
     9121 aaaacgcgcc gctggatatg tataaagaag tgaataccgg caccaatctg ccagcgcaga
     9181 tcgatcttta tgccgttgat ggcgacgaat acaaattcct ctgtatcgca aaaggtggtg
     9241 gttcggcaaa caagacgtat ctctatcagg aaaccaaagc gttactgacg ccggggaaac
     9301 tgaaaaatta cctggttgag aagatgcgca cgctgggtac cgcggcctgt cctccgtatc
     9361 atattgcgtt cgttattggt ggaacttctg cagaaacgaa ccttaaaacg gtgaaactgg
     9421 cttccgcgaa atactatgat gaactgccaa cggaagggaa tgagcacggt caggcgttcc
     9481 gcgatgtgga actggaaaaa gaattactga tcgaagcgca aaatctcggt ctgggtgcgc
     9541 agtttggtgg taaatacttc gctcacgaca tccgcgtgat tcgcctgcca cgtcacggcg
     9601 catcctgtcc ggttggtatg ggtgtttcct gctctgctga ccgtaatatc aaagcgaaga
     9661 tcaaccgtca ggggatctgg atcgaaaaac tggaacataa tccaggtaaa tatatccctg
     9721 aagagctgcg taaagcggga gaaggcgaag cggtgcgcgt tgaccttaac cgtccgatga
     9781 aagagatcct cgcacagttg tcgcagtatc ccgtttctac acgcttatcg cttaacggca
     9841 cgattatcgt tggtcgtgat attgctcacg ccaaactgaa agagcggatg gataacggtg
     9901 aagggctgcc gcaatacatt aaagatcatc cgatttacta cgcgggtccg gccaaaacgc
     9961 cggaaggtta tgcctccggt tctcttggcc caacgaccgc cggacggatg gattcttatg
    10021 tcgatcaact gcaagcgcag ggcggaagta tgatcatgct ggcgaaaggc aaccgcagcc
    10081 agcaggtgac ggatgcctgt aaaaaacacg gcggcttcta ccttggcagt atcggtggtc
    10141 cggccgctgt attggcgcag ggaagtatta agagcctgga atgtgttgaa tatccggaac
    10201 tgggaatgga agccatctgg aaaattgaag tggaagattt cccggcgttt atccttgtgg
    10261 atgataaagg aaatgacttc ttccagcaga tacaactcac acagtgcact cgctgtgtga
    10321 aataaacaga gccgcccttc ggggcggttt ttttacatgg cacgaaagac caaacatttg
    10381 ttatcaaata gtaaataatg agtgagctaa aagttgctta acgaaagcaa aacagaaaga
    10441 aaaaattaat caggtgagga gcaggccatg aatacagtac gcagcgaaaa agattcgatg
    10501 ggggcgattg atgtcccggc agataagctg tggggcgcac aaactcaacg ctcgctggag
    10561 catttccgca tttcgacgga gaaaatgccc acctcactga ttcatgcgct ggcgctaacc
    10621 aagcgcgcag cggcaaaagt taatgaagat ttagacttgt tgtctgaaga gaaagcgagc
    10681 gccattcggc aggcggcgga tgaagtactg gcaggacagc atgacgacga attcccgctg
    10741 gctatctggc agaccggctc cggcacgcaa agtaacatga acatgaacga agtgctggct
    10801 aaccgggcca gtgaattact cggcggtgtg cgcgggatgg aacgtaaagt tcaccctaac
    10861 gacgacgtga acaaaagcca aagttccaac gatgtctttc cgacggcgat gcacgttgcg
    10921 gcgctgctgg cactgcgcaa gcaactcatt ccgcagctta aaaccctgac acagacgctg
    10981 agtgaaaaat cgcgtgcatt tgccgatatc gtcaaaatcg gtcgaaccca cttgcaggac
    11041 gccacgccgc taacactagg gcaggagatt tccggctggg tagcgatgct cgagcataat
    11101 ctcaaacata tcgaatacag cctgcctcac gtagcggaac tggctcttgg cggtacagcg
    11161 gtgggtactg gactaaatac ccatccggag tatgcgcgtc gcgtagcaga tgaactggca
    11221 gtcattacct gtgcaccgtt tgttaccgcg ccgaacaaat ttgaagcgct tgcgacctgt
    11281 gatgccctgg ttcaggcgca cggcgcgttg aaagggttgg ctgcgtcact gatgaaaatc
    11341 gccaatgatg tccgctggct ggcctctggc ccgcgctgcg gaattggtga aatctcaatc
    11401 ccggaaaatg agccgggcag ctcaatcatg ccggggaaag tgaacccaac ccagtgtgaa
    11461 gcattaacca tgctctgctg tcaggtaatg gggaacgacg tggcgatcaa catgggtggc
    11521 gcttccggta actttgaact gaacgtcttc cgtccgatgg tgatccataa tttcctgcaa
    11581 tcggtgcgct tgctggcaga tggcatggaa agttttaaca aacactgcgc agtgggtatt
    11641 gaaccgaatc gtgagcgaat caatcaatta ctcaatgaat cgctgatgct ggtgactgcg
    11701 cttaacaccc acattggtta tgacaaagcc gcggagatcg ccaaaaaagc gcataaagaa
    11761 gggctgacct taaaagctgc ggcccttgcg ctggggtatc ttagcgaagc cgagtttgac
    11821 agctgggtac ggccagaaca gatggtcggc agtatgaaag ccgggcgtta atctgcaaca
    11881 tacaggtgca gccgtggaat gatcaaacga agcggctgcg cctgaggttt ataacggtgc
    11941 tgcacattgt cggcatcgta atttaacaac tcaccaacgt ccggcacgcc cgcgccatta
    12001 tcccgattaa tcagtgcaat cagtggtgta gggcaggcgt gttggacttg tttttgatct
    12061 cctttgtacc agacgcgggc aatcggctgc accttcactg gacgtttgat ttttaacttc
    12121 gcgttctgtg gcagggcagc gatatcctga tactctcgct ccagttttct ttgccactcc
    12181 tcgcgcgtcc acggtgcgac actgcgtggt gatttcaggc ttttttccag ctgtgccagg
    12241 acttcatcac gatgcaaatt cttaatgata tgtttattag cccaaccaaa gcgtaaagtg
    12301 gcggggtcgt gcagaacggt gagcgtgcgg taagcattaa gggtgatcag ccccggcaaa
    12361 tgacgatgca cccattcaaa acgtgccgcg gtggggagtt ctgattcaac cgtgacgata
    12421 tgctcgaacg tggtcttgag tttattgatg tgctgaatat gactgaccaa cgctgcttgc
    12481 gaaaggttat cgacctggta acacaacacg ccaggcagac ggacagcggc cttgctgctg
    12541 cgattttcgg actgttgttg aataaataaa tggcggaaat gacgcaacgc cagcgactgc
    12601 gcgtcgttgc cgagatgttg ttttacctca atacgattaa gcggattatg ctcatcctct
    12661 ttttttacct ccggcaaaga gaacacgcgg gcaaccaata gcttgtgttg ctcaagatga
    12721 gcggcaaatg tagccagctc ttgttccatc tggcgaaagg tagtgttgag tcggtctacg
    12781 agatcgtaac gcgccataat attaaccact ttagttacaa catacttatt ttatagcaca
    12841 gtcgtggtgg cttgaccatc gtgcgcgtag tgtcaggcag aggtaaattg cgggatgtta
    12901 tgccataacg gccagctaaa cgagaagcga gcaccaccca gttcgctgat gtcacaatta
    12961 accgtaccgc ccattgccag tgctattgag tggacaattg ccagccccag cccgcagccg
    13021 ccggttgagc gatcccggct gggatcgagg cgaacaaaag gttcaaagat atgttcgcgg
    13081 ttttctgggg caatccctgg gccatcatcc tcaacaatta atgtcgctct attccccgac
    13141 agtagcaggc tggtttcaac cgttgaatgg cagtagcgca gggcgttatt gagcaaatta
    13201 tccagcacgc gctccattaa gcgcatatcc aacgccgcat aatggccttg catgagcgtt
    13261 tttatccgta ccgttttatc gggcgttact gcctgaatat ctgccagatg cgttgacagc
    13321 cacaacggca ggtctggttc gctaagatga agctcgtttt gtgggcgatc gagtcgggca
    13381 taagtcagca gctcttcaat taaagcttca agttgactga tatcacgatt caacgcctgg
    13441 gattcggcgg cgctcaggtt atcgctcatc tccagtcgat aacgcaggcg cactaacggt
    13501 gttcgcagtt cgtgagcgat accgtcaata agctgttttt tgctggcaat taaggcgttg
    13561 atattgtccg ccatctggtt aaatgcgata ccaagtcgtt caaagctcga accctcatca
    13621 aagtggatac gttcattgag atgcccatcg ccaaatcgtt gcgccgctgc ttccagtttt
    13681 aacatatcct gccagtgcgg acgcatccag ataaacaccg gaaaggcgag ggaaatagca
    13741 ataaaagcga tcagggcgat atccagcaat cgcatttgat ggaggtaata aagataagga
    13801 acaggaccaa ctgccagcac gtagtggctg cgcgggatac gctgcaaaaa cgtgtactga
    13861 tcgtccaggg cgacaatttc gccgccacgc agtcggtgca tggaaatatc atcaagatgg
    13921 tatttactca gtggctcgac acgcagatcg aaagagagat ttaaatccat ctctttcagc
    13981 gtcttacccc agtcgtgtgg ggggatctct cgcaattcgc tgcgcatcag atacagcgaa
    14041 ctgttcatca aatcatccag cgactgtttg cccgcgcgtt cggcggtaaa tttgtacacc
    14101 agcccaacca gcagagacat cacaaggaag cagacaaaca ataacaggta aaactggata
    14161 aacaattttt tcattggcat cgcccgcaac agaatactat tcgtattaat tcacgactgt
    14221 ggagcaatgt aaaccgccaa ctggataaga ccttgtttgg aagataatgt ccggaacaga
    14281 cggatgtatc cccggtgtat tcatattcag taaattcaat tttacaccgg ggcttgttta
    14341 ttatgctaag gtttaattta acgtgacgct ttgttgttct ttagtctctc tatccttaag
    14401 aagacgggct gatattacac cggacattat tgaaccattc acattaagcg ctgttcttgc
    14461 catatcaacg atcggctcta tggcaataaa aataactact aatgcgacag ggaaattaag
    14521 tgttgaaagg acaatcagcg ccgcgaatgt cccaccacta cctacgcctg ctacccctat
    14581 tgagccaagc gcgatggcag gtaaaagagt tgcgaggaat tgcaatgaga agggatcgat
    14641 acccattgtt ggagcaatca tcgccaccat cacggcagga taaatcccag cgcagccatt
    14701 ttgtcccatg ctggaaccaa aagacgctgc aatattagca atcgtatttg gaacccccaa
    14761 tttttccaat gcagaaatag ccagaggaat tgaagccgca ctactgcgag aaacaaacgc
    14821 aaaggtaagt acttgccaga cggtcttgta atagttaacg gggtttttac ctattaacag
    14881 caaaatgccc gagtgaacaa taaacatcac aaaaacggcc agataacagg cgacgatgaa
    14941 cccgagcaaa ttggtaaact gtgaaagctg atacgttgag aagactttcg ccattagtgc
    15001 cattacccca taaggcgtca gcgcaatgac aatacggacc attttcatta cccatatttg
    15061 aatcgcgttg atccccgcgc tcaatttttc gccctctgct ggtgcatctt tctttacttt
    15121 taacagcgca ataccagcaa gaattgtaaa gatgacgatg ccaataaccg aaacacttct
    15181 tgccccggtc agatcaagga agaggttggt tgggatcaat gacgtaatga gtcgcggtaa
    15241 agataccgca gctgttttcg agacgtcatc gctggttaaa gaggattgca tatgaccaaa
    15301 ggcgctggca tcaaggccaa ttgcactggc aatagccagg ccaactaaac ctgcaatcat
    15361 gactaaacac aacatacagg caacaatagt taaagacatc ttgccaatac cagcgctatt
    15421 ttcgagctta ttaatcgctg aaagaatcga aatgaatatg agtggaacgg caacaagttt
    15481 taataaatta acataaccgt taccagcaat attataccat tcgataacgg tttcactgct
    15541 tgtctcagga atgaaatgca gggagatacc aatgatagcc cccaggaaaa tcgcgataaa
    15601 aacacttttg gaaagggatt cgctcttttg atccgttttg aaaatccccc atgagcagat
    15661 caaaaaaagc acaatatatg tgaaagttgt aaccgttttt taagcctctt gcctcattca
    15721 gcacctgaaa tgccggaaag agtttgttgt aaaaaatctg cgccataaga cgcagggacg
    15781 aggcgattat agagaaattt tttctcattt tcatatcaaa tgtagggggt ttggaatttg
    15841 aataagctct tattaattga attcgttaat tgttctcttt ttcagatgaa caaatattag
    15901 tcatctgatt gtttattgtc actatcccat gcatgaggcg caaaaagata gcctttgtta
    15961 cgcacagttt taatgcgata aggttctgcg gcgttatcga gcagtttttt tcttaaccgc
    16021 gaaatagcca cgtccacgct acgatccagt ccgtcataac tgacgccgcg taaatttttc
    16081 agcaatgcat cgcggtccat gatttgcccg gcatgcgtag ctagttccca caataattcg
    16141 aaatcagctg tcgagagcga gatttcagtg ttagccaggg tgactacgcg gttgatgggg
    16201 tcgatagtta acgtgccgaa atgcagggct ttgtagggag taagagacgt ttcctgaaga
    16261 cctttggtca gtgtggcttg ctcattctga cgcaaatgca aacgtaaacg cgctagcaaa
    16321 acagcagggg gcgtcgtttt gagaatatag tcgcaggcac ccatttccag tgccaggatg
    16381 tggttcatat cgctatcgag agaggttaga agaacaatcg gtccagacca ctttgcgcgt
    16441 aaatcacgac aaatggtcat gccgtccttg cctggtagca tgatgtcgag taacaccaaa
    16501 tccggatttt ctcgcaaaat ggtttcttcg gcctggtcgc cgcgcggttc tacggtaacc
    16561 tgcatatcat gttttgccag gtacgcggca atcagtgaac cgacttccgc atcatcttcc
    16621 acaaatacga tagtgttcat aacattcacg gtagatataa aaacgtcaca atacaccgcg
    16681 ctgtttttac ttactatcaa tcgtttctaa acaagttttc atcaggtatt ctactgatga
    16741 cttgttatgg aagtgttaag gtaaaaagat ggggctggta atcaaagccg cgctcggtgc
    16801 gctggtagtg ctgttgattg gtgttttagc aaaaacgaaa aattattata tcgccgggct
    16861 gattccactt ttcccgacct ttgcgcttat cgcgcattat attgttgcca gcgaacgcgg
    16921 cattgaagcc ttacgcgcaa ccatcatttt tagtatgtgg tcgattattc cctattttgt
    16981 ttacctggtg tcgctgtggt atttcaccgg aatgatgcgc ttgcccgccg cttttgttgg
    17041 ttctgttgcg tgttggggga taagcgcatg ggtgttgatt atatgctgga taaagctgca
    17101 ttaacgcaga tgacgaccgc catcaagtgg gaaactgcgt ccggtgacaa agcaactggt
    17161 aagtaagtaa tcgaccaggt cgatcacttc tttctcgcca ggcgcggttt tcatcagtga
    17221 tttattcagc gcctgttgtc gatattcggc atcatcatgt tcattaaaca ggatcagcga
    17281 tggcgcaata gaattcactt tcacttccgg tgccagcttg cgggcaaacg agcgggtcat
    17341 attatccagt gccgctttgc ttgcagcata cgcaacatgt ttgtcgctac cgcgctccac
    17401 cacataatcg gtaaagtgaa tgatatcgct ggcggcgtgt ccgtgcccac gcagtaatct
    17461 ttccagcgca tggttgagca ggtatggggt attaacgtgg atctgcatca tgcaagccag
    17521 tacgtcggcc agtggcgcac ccggtttttc cgcaatccac gcactggcgt tatgcaaaat
    17581 agcacgcaga ccatgggtgg tttttagtac ttcatcggca aacgccatca caccgtcgtt
    17641 ggtcgaaaaa tcagcctgaa tacactgcgc acctgcatta atcagtccat caatggctgg
    17701 atagtgtgtc cgatagctga caatcaccgg ttgcttttga ttaatgaaat gccatgcgag
    17761 ggcgaggccg atgcgacgac ctccgccagt aattaatatt ggcaagggct gggttttacc
    17821 catcgttatc tcctttgcta tccaacgatg ggatgaacgt tatcccacca gcatccaggt
    17881 cgccggaact gaggcaatta acagcatgcc aatcagcacc atttcctgac ggttcagtac
    17941 attatcatgt gtatgcgttt tgcgcgcata caggaaaacc agcagccccg gtgcatacag
    18001 aacaacggat aacagcaagt gcattggacc agaggcgtac aataaccata agccataaat
    18061 gcaggcaccg acacctaccg ctttatgcag tggacgggtt gcgattttca gcaagaacgc
    18121 gccgaccagg aaatagggca ccagaatcat ttctgaggcg atggtcagca acgtgttgta
    18181 atcggaaccg gtcagccaga tgagtaccag acaaatttgc acgcagatgt tggtcaacca
    18241 cagtgaggcg gatggcgcag cttgtgcgtt ctggcgcgca aaaatgcgtg ggaatgcttt
    18301 atgagtggca gccaggaacg gaacttctgc cgccatgatg gtccagctta agtacgcgcc
    18361 gcaaacggaa acgatcaaac cggcagcgat gatgatttcg ccccatggtc ccatcatttc
    18421 caccatcaga ccggccattg acgggttacg aatttcagcc agttcaggac gtgccaccac
    18481 acccagcgaa agcagcgtta ccagcaagta aacgcccaga gcggagagaa ccgccagcag
    18541 tgtcgctttg ccaacatcac gtttattacg cgcacgcgca gaaacaacca cggcaccttc
    18601 cacaccaatg aaaacccaca gggtgatcag catggtgttt ttcacctgtt cccaaacagg
    18661 tacgccaagt gcaagtccgg tgaagtcgag cttgaaggta tccagtttga acatcatcat
    18721 cgccagcaca acaaacagac ccagcggcaa caattttgcc agtgtcgcca ccaggttaat
    18781 gctggcagcg gtttgcaccc cgcgcaggat caaaaagtga acaatccaca ataaagccga
    18841 tgcaccgact atcgattgcc aggtattacc atcgccaaac aggcgcaatt ccggcgtgtc
    18901 cgtaaaaaag cttaacgcgg aaaaaacgat caccagatag gagacgttgg cgatgaccgc
    18961 gcacagccag tatccccatg cggaacaaaa gccgattagc tcgccaaacc cttcgcgggc
    19021 ataggtaaag ataccgccat caagttcggg acgaatgcgc gtgaggatca gcatggcaaa
    19081 ggccagcaat aaaatgccag cgccagtaat accccagccg atgagcagtg ctgccgggct
    19141 ggcaactgcc gccatatttt gcggcagact gaaaacaccc gcgcccagca ttgagcttaa
    19201 taccagcgcg gtgagtgcgc tcagtcccag tttcttttcc atcgatatcc agtgattaaa
    19261 acgtgataag aagaataaat attagggcaa aacgcataaa aatggagttt gccactgaag
    19321 cgcgggattt tacgaagaga tgtgtctgca tgcaatggtc gcggggaaat ttgtgactaa
    19381 aaatgcgaaa ggcggcatca agccgcctta taggagtaac tgaatagtta tttatacaga
    19441 tctgcgctga cggtcaggtt attaccacgt ttctgccact ggcgggtgat gtggtaatac
    19501 ttagcacctt tcttcgcggc acgtttcgca acctgatagg agacttcggt catgttgccg
    19561 tagttgccag agaatttgat gctgtcgaac gggaccatca tcgctgctgt cgctttgttc
    19621 agttcttcga ctttagtgcc atccgggagc gtgacggtgt aacgcccgcc ttttgatgac
    19681 tgggtttcaa agaagcgacc gacttcagaa cttggtgatg cggtagtcgc aacacccggg
    19741 atctcaactt tcttcgcggc ttcgccaccg gcagccagag ctgcacgtcc tgcttcggaa
    19801 tctgccggga tcacatccgg gctctggacg atacgtttct tagcatcttt tttatagatg
    19861 aatgcagtaa tacgctggtt gccgccctgg ttggcatcga tttgacgaac gatgtagaaa
    19921 gagtaggcac ctttcgcttt tgccgctttg gtgatggcat cattgacttc tggctggcta
    19981 cgatagaagc cctggacggt gaccgtgtca aacggttcaa tcagaacagc ctgatctttc
    20041 ggcagttcga caacaccgtt aattacgcga ttacttgttt cttctgcttt ttcagcatcg
    20101 gctttataga ggtcagcgac cacacgccag ttaccgctgt taccaaaatc agaagtgtcg
    20161 acaacataaa aagaggcggc accttcttta tctgcgcgac gagaaacggc tttcaccgct
    20221 tcgccaatag cattaaaacg accggtaacc actacacggt caaaaggttt aaccgctgcc
    20281 gcttgctccg gtgtcagttc tgttgctgcg ttaacggaga atgccgtagc agaaagcagt
    20341 gccgacgcca ggagggtgtt cttaagcttc ataaaaataa tccttcgcct tgcgcaaacc
    20401 aggtactggt attgttatta acgagaaacg tggctgatta ttgcatttaa acggtgtaac
    20461 tgtctgcgtc atttttcata tcacattcct taaaccaatt ttaatcctgc tcaaatgacc
    20521 gtctatgctt aaaaaacagc cgtatcagca tcattactac tgaagcaact gaattgtata
    20581 agttaattta atgttaagta gtgattcgtg ccggggcgat gtctcgtttt acccgaccgt
    20641 cgaagacaat tatcagtctt tatccggcgt tctaaggtgt ttatcccact atcacggctg
    20701 aatcgttaat attttgcgag ttcacgccga aatactgatt tttggcgcta gatcacaggc
    20761 ataattttca gtacgttata gggcgtttgt tactaattta ttttaacgga gtaacattta
    20821 gctcgtacat gagcagcttg tgtggctcct gacacaggca aaccatcatc aataaaaccg
    20881 atggaaggga atatcatgcg aattggcata ccaagagaac ggttaaccaa tgaaacccgt
    20941 gttgcagcaa cgccaaaaac agtggaacag ctgctgaaac tgggttttac cgtcgcggta
    21001 gagagcggcg cgggtcaact ggcaagtttt gacgataaag cgtttgtgca agcgggcgct
    21061 gaaattgtag aagggaatag cgtctggcag tcagagatca ttctgaaggt caatgcgccg
    21121 ttagatgatg aaattgcgtt actgaatccc gggacaacgc tggtgagttt tatctggcct
    21181 gcgcagaatc cggaattaat gcaaaaactt gcggaacgta acgtgaccgt gatggcgatg
    21241 gactctgtgc cgcgtatctc acgcgcacaa tcgctggacg cactaagctc gatggcgaat
    21301 atcgccggtt atcgcgccat tgttgaagcg gcacatgaat ttgggcgctt ctttaccggg
    21361 caaatcactg cggccgggaa agtgccaccg gcaaaagtga tggtgattgg tgccggtgtt
    21421 gcaggtttgg ccgccattgg cgcagcaaac agtctcggcg cgattgtgcg tgcattcgac
    21481 acccgcccgg aagtgaaaga acaagttcaa agtatgggcg cggaattcct cgagctggat
    21541 tttaaagagg aagcgggcag cggcgatggc tatgccaaag tgatgtcgga cgcgttcatc
    21601 aaagcggaaa tggaactctt tgccgcccag gcaaaagagg tcgatatcat tgtcaccacc
    21661 gcgcttattc caggcaaacc agcgccgaag ctaattaccc gtgaaatggt tgactccatg
    21721 aaggcgggca gtgtgattgt cgacctggca gcccaaaacg gtggcaactg tgaatacatc
    21781 gtgccgggtg aaatcttcag tacggaaaat ggtgtcaaag tgattggtta taccgatctt
    21841 ccgggccgtc tgccgacgca atcctcacag ctttacggta ctaacctcgt taatctgctg
    21901 aaactgttgt gcaaagagaa agacggcaat atcactgttg attttgatga tgtggtgatt
    21961 cgcggcgtga ccgtgatccg tgcgggcgaa attacctggc cggcaccgcc gattcaggta
    22021 tcagctcagc cgcaggcggc acaaaaagcg gcaccggaag tgaaaactga ggaaaaatgt
    22081 gcctgctcac cgtggcgtaa atacgcgttg atggcgctgg caatcatcct gttcggttgg
    22141 ctgtcaagcg ttgcgccgaa agagtttctt gggcacttca ccgttttcgc gctggcctgc
    22201 gttgtcggtt attacgtggt gtggaatgta tcgcacgcgc tgcatacacc gttgatgtcg
    22261 gttaccaacg cgatttcagg gattattgtt gtcggagcac tgttgcagat tggccaggga
    22321 ggctgggtta gcttccttag ttttatcgcg gtgcttatag ccagcattaa tattttcggt
    22381 ggcttcaccg tgactcagcg catgctgaaa atgttccgca aaaattaagg ggtaacatat
    22441 gtctggagga ttagttacag ctgcatacat tgttgccgcg atcctgttta tcttcagtct
    22501 ggccggtctt tcgaaacatg aaacgtctcg ccagggtaac aacttcggta tcgccgggat
    22561 ggcgattgcg ttaatcgcaa ccatttttgg accggatacg ggtaatgttg gctggatctt
    22621 gctggcgatg gtcattggtg gggcaattgg tatccggctg gcgaagaaag ttgaaatgac
    22681 cgaaatgcca gaactggtgg cgatcctgca cagcttcgtg ggtctggcgg cagtgctggt
    22741 tggctttaac agttatctgc atcatgacgc gggaatggca ccgattctgg tcaatattca
    22801 cctgacggaa gtgttcctcg gtatcttcat cggggcggta acgttcacgg gctcggtggt
    22861 ggcgttcggc aaactgtgtg gcaagatttc gtctaaaccg ttgatgctgc caaaccgtca
    22921 caaaatgaac ctggcggctc tggtcgtttc cttcttgctg ctgattgtat ttgttcgcac
    22981 ggacagcgtc ggcctgcaag tgctggcatt gctgataatg accgccattg cgctggtatt
    23041 cggctggcat ttagtcgcct ccatcggtgg tgcagatatg ccggtggtgg tgtcgatgct
    23101 gaactcgtac tccggctggg cggctgcggc tgcgggcttt atgctcagca acgacctgct
    23161 gattgtgacc ggtgcgctgg tcggttcttc gggggctatc ctttcttaca ttatgtgtaa
    23221 ggcgatgaac cgttccttta tcagcgttat tgcgggtggt ttcggcaccg acggctcttc
    23281 tactggcgat gatcaggaag tgggtgagca ccgcgaaatc accgcagaag agacagcgga
    23341 actgctgaaa aactcccatt cagtgatcat tactccgggg tacggcatgg cagtcgcgca
    23401 ggcgcaatat cctgtcgctg aaattaccga gaaactccgc gctcgtggta ttaatgtgcg
    23461 tttcggtatc cacccggttg cggggcgttt gcctggacat atgaacgtat tgctggctga
    23521 agcaaaagta ccgtatgaca tcgtgctgga aatggacgag atcaacgatg actttgctga
    23581 taccgatacc gtactggtga ttggtgctaa cgatacggtt aacccggcgg cgcaggatga
    23641 tccgaagagt ccgattgctg gtatgcctgt gctggaagtg tggaaagcgc agaacgtgat
    23701 tgtctttaaa cgttcgatga acactggcta tgctggtgtg caaaacccgc tgttcttcaa
    23761 ggaaaacacc cacatgctgt ttggtgacgc caaagccagc gtggatgcaa tcctgaaagc
    23821 tctgtaaccc tgacggcctc tgcggaggcc gtcactcttt attgagatcg cttaacagaa
    23881 cggcgatgct ttgacctccc gctgtttgtt caagcgcaat tttgacaata attgtcaacg
    23941 gcacggaaag cagcataccc accggtccta acaaccatcc ccaaaaaatc aacgacaaaa
    24001 ataccaccaa tgtcgaaagc cccagcccac gtcccatgat gcgcggctcc agaatattgc
    24061 cgaagaccag attaatcagc agatatcccg ccagcaccag caacgcttcg tagaagccat
    24121 taaacaccag tacctgagcg atagggggga ttgccgcgag aactgaacca atattaggga
    24181 tgtaattaag cgcaaaggcc agcaatcccc agacaaaagc gaagcgaaca tcgagtgcgg
    24241 cgagcatcgc ccaggcgacc aggccggtga tgatgctgat ggctgttttc agcaccagat
    24301 aatgagaaac actgtcaatc gcacgttgaa tcgccgccat cccttcaacc ggacgcgcca
    24361 tcatttgctg aaattttccg ggcaattgtg gcacttcgag cagcataaac agcaccgtca
    24421 gcagcaataa aaatattgat gacatggcat tagataactg cgtcaataag ttggtgagca
    24481 acgtcatcgc cgcgttcgga tcaatgtaat gcgccagttg gtcaactgag acgtcaatcc
    24541 ctacgcgttg caacaacggc tcaagagctt gcagcggcgt cataatagag ttgcgatatt
    24601 gcggtaacgt ccacgtcaac tcgttgagcg cggaacccag ataagctaat agcaacacca
    24661 tcgccatcac gatgattgtc atcaaaatcg acactgccag tacacgtggc acacgccagc
    24721 ggaccatatg ttgcaccagc gggttaagaa taacagcaat aaataatgcg agaataaacg
    24781 gcacgatgat ctcggcggca aaacggatac cgcagagaat aatgaccagc attcccaaca
    24841 taatgacgat ttttaggcca ttgagcgtga tgatcggctt tgccatgctt gtccccgttt
    24901 ttcttccttt gctaataata atgtcttttt cgctccgtca tcataacgaa aatcaaaccg
    24961 ctcaggtaaa gaagtgaaaa tattttgagt taattcttaa gctatgatac aaatcaggcg
    25021 tgttcaacta ccgaggacaa ttatcatctg cgatgacgag aagcaacact gcggataatt
    25081 gtaatattat ggacaatttg ttcaggacgt tattttctac atttacccat cttcgtacct
    25141 catccactat cttgctcgta ggtgagcagc actggcgcaa cgcgctttag tcctttcttc
    25201 tgcctaagct tgccctatgc gcagggcttg accactttac tttggtttcg ctgaattaag
    25261 cgaaaattaa aatagttctc ttgcaggaga aggacaatgt atatttattg gattttatta
    25321 ggtctggcta ttgctacaga aattaccggt acgctgtcaa tgaaatgggc gagcgtcagt
    25381 gagggaaatg gcggctttat tttaatgctg gtgatgattt ctctgtcgta tatatttctc
    25441 tctttcgccg ttaaaaaaat cgccttaggc gtagcttatg cgctgtggga aggtatcggt
    25501 attttattta ttaccttgtt tagcgttttg ttattcgacg aaagtttatc gctgatgaaa
    25561 attgccgggt taaccaccct ggtcgccggg atagtgttga tcaaatcagg tacccgtaaa
    25621 gcgcgtaaac ctgaactgga ggtgaaccat ggcgcagttt gaatgggttc acgccgcctg
    25681 gctggcattg gcaatcgtgc tggaaatcgt tgctaacgtc tttttgaaat tttctgacgg
    25741 ctttcgtcgc aaaatatttg gcttgctctc cctggcggcg gtgctggcgg cctttagtgc
    25801 gctttctcag gccgttaaag ggatcgactt gtctgtcgtt tatgcattgt ggggcgggtt
    25861 tggtattgcc gccacgttag ccgcaggttg gatcttgttt ggtcaacggt taaatcgtaa
    25921 aggctggatt ggcctggtct tgctgttggc tggaatgatc atggtgaaac ttgcctgatg
    25981 aagacgctgc ccgcgctgtc gggcagcgtt tgaacattat ttttgcgaca gttgatccag
    26041 cttgtcgcga aaaccggtaa cagaaatggc ccggttatcg gcgcgccagc gatctttcgc
    26101 ggcaggagcc gaactttgca ccccaattaa ttgccagccg tcatcggtat gcaacatcag
    26161 aggcgaaccg ctgtcacccg gcaaggtatc gcactgatgt gacatcaccg acgtttgcgc
    26221 ccagccagtc acttcacagt tttgatgact gtataacgta tcgagatgat cttcagggta
    26281 tcccgcctga gtcactttac gaccagccga ttttaatgcg gcagtaagcg cggctttatc
    26341 tccctcaaat aacggcaacg gtgtaatgcc agaaggggga ttacgtagca caatcaatcc
    26401 gaagtcccac ggtgcagctg cgggaggaac aatccaacca tccccatctg cttttaaccg
    26461 ctttcccagt gtcggatcaa cgcggccttc tatgtcgtgg atctcatagc gccaaagacc
    26521 tttatttgac acaaaacgca gcgctaccgc cttatccgct ttacccttag gcggcgtcaa
    26581 taaacagtgt cctgccgtta atgccagatt gggtgcaatc agcgtcgccg tacataaatt
    26641 gccgctggcc gtttccagtt gcccaaccgc atcccacggt gattgggtcg tgtcattcac
    26701 tggcacacga tcatcatgac caaaaaacag ggtgctgacc tcatcgtttg ccgatttggc
    26761 aacgtctggt ttatctgcaa acacaaaagc agacgtcaaa ctaattgcac ccaacactac
    26821 agcaatggtt gtacgcatat cacactctgg tgggtaatta tgattattaa aagcaaaccc
    26881 tcataaatac tatagacggg acgggatgaa agtgggagta aaatcagata actacagtca
    26941 ggaaagataa aaacgggcgg caatcagtgc gcataaaata aggatgataa gaatgaactc
    27001 aaagcgataa cggcccacca tactaccctc cgctaaaggc ggtgctaaac gttacccgct
    27061 tagcaccgta attttcaggc gcgagggggc gcgccagcat tactgttgaa aacttacgct
    27121 gcgggttgtg cagcaggttt tgccggttgc tgatggccgt gtttcccggc gtgtttcttc
    27181 gctgcctgcg ctttttgttc aggggctttc tgttcagctt tcgtattttt atgatgcttt
    27241 ttagccgcct gcgctttctg ggcaggtgct gctttatgct gttttttatg atgtgtagtt
    27301 ttcgctggcg ctgctttggt ggtcgtcgca gtcggagcag gtgtggtcgc agtctctgca
    27361 gcaaaggcgg cagaagacag acccatagca gcggcaacaa ccagagctaa tacttttttc
    27421 attgtcatac cctcaatttg ttttttcatt taaccccact gcggggccgt tgaaataact
    27481 atatccctgg gaatggcaga cttccgtgag tggttggttt cagcgtgtaa cgatatgtac
    27541 aaacgctgaa taaattacag cgttgataat aacgtattgt gacttaagga aaatttagct
    27601 accaataata gtagtcttga tcgggttaac tattatacta cttacaaagc ggtagaataa
    27661 ctgcgcatca gtaataaatg acacacagca aaatgaatcc gtttatttgg gtacttataa
    27721 tcctgatgac gctagatgcg ctgcgggaat tggctggcgc ttcgtctatt ttaggatggc
    27781 tattaacgtt ggtttgagca ggcaataagt ccggacgggt atttaccgca gtccggactt
    27841 atttttcagg cgtgcagacg acgatgcaaa cgcgtcccga ccagcagagc aatgaccagc
    27901 atcagagcaa taaatgcgcc gacgccgttc cagccatagt tatgccagaa aacaccaccc
    27961 agcgtcccgg caatactcga ccccagatag taactgaaca gatacagcga ggaggcctag
    28021 cctttagcgc gttttgcgcg ggggccgatc cagctgctgg ctactgagtg ggctgcgaag
    28081 aatccggctg agaagagtaa cattccggca aagatcagcc acagcgagct aaataaggtc
    28141 atcagtaaac caaacggcat aactcccgtc gaaaacaaca tcaccggacc gcgcccatag
    28201 cgggtggtca tggttccggc tttgggtgag ctccatgtac cggtcaaata agccagcgat
    28261 aataagccaa ccacggcctg actgacatgc tagggggaaa gcatcaaccg atagccgatg
    28321 taattaaaca gcgtgacgaa cgaccccatc agcaaaaagc cttctgcgaa caataacggt
    28381 aatccccggt cacgccagtg cagacgaaag ttgataaata acgtcttagg gcgcagcgaa
    28441 gtcgggcaaa aatggcgtga ttcagggagg attttccaga acatcaacgc cgaggccagc
    28501 gcgaaacaac cgattgccgc cagagcaatt cgccagttga aaaagtccgt gaagacacca
    28561 ctaattaagc gtccgctcat gccgccaatt gagttgccgc tgatatacaa ccccatcgaa
    28621 aaggccacga aactggggtg gatttcctcg ctaagataag tcatgccaac agctgccacg
    28681 ccacttaacg aaagcccaat caaggcgcgc ataatcaaaa tgccgtgcca gctggtcatc
    28741 attgtcgaaa gtaacgtaca aatggaggcc aacagtagcg ccgtgaccat cactggtttg
    28801 cgaccaatgg catcggatag cgggccagta aatagcaaac cgatagccaa catcgccgtg
    28861 gaaatggaca gtgaaatact actgttcgcg ggcgttaagc caaactcctg cgaaagcacc
    28921 ggaaggatag gctgcacaca atagagaagt gcaaaggttg ccagtccggc agagaacagc
    28981 gccagggtga cgcgcataaa ttgcggcgta ccgcgtttaa taaattgatt tggctgagaa
    29041 atgctttgct tgtcagtgtc gcttgccgga gcgccatcaa cagttgtagt acggctcact
    29101 tgaaatcctt gctaaatatg cctgtggatc aggcttatac atagggtagg aaaatcgaat
    29161 tgttctgtct aatatattaa taatctcaaa taagatgttt taaatatgaa tattgaactt
    29221 cgtcatctgc gttactttgt tgctgttgcg gaagagctgc attttgggcg cgcggctgcc
    29281 cgcctgaata tttcgcaacc gccgctaagt cagcagattc aggcactgga gcaacaaatc
    29341 ggtgcccgac tgctggcacg aactaatcgc agtgtattgc tgacggcagc aggaaaacag
    29401 tttcttgcag atagtcggca aatcctgtct atggtggatg acgctgccgc tcgcgctgaa
    29461 agactgcatc agggtgaagc gggggagttg cgtattggtt ttacttcgtc cgctcctttt
    29521 attcgggcgg tgtccgatac gttatcgctg tttcgccgtg attatcctga tgtccattta
    29581 caaacccgcg aaatgaacac tcgcgagcaa atcgctccgc tcattgaagg aacgctggat
    29641 atgggattgc tgcgtaacac agcgttaccg gagacgcttg aacacgcagt catcgtccat
    29701 gaaccgctta tggcgatgat cccgtacgat catcccctgg caaataatcc gagtgtaaca
    29761 ctggctgaac tggctgaact ggcgaaagaa ccctttgtct tttttgatcc gcacgtcggg
    29821 actgggctgt atgacgatat tctcggactg atgcgacgtt accatttgac gcccgtcatc
    29881 actcaggagg tgggcgaggc aatgaccatc atcggtctgg tttccgccgg tctgggtgtt
    29941 tcaattttgc ctgcgtcatt taaacgtgtt cagctcaacg aaatgcgctg ggtgccgatt
    30001 gctgaagagg atgcggtttc tgaaatgtgg ttggtctggc cgaaacatca tgaacaaagt
    30061 ccagctgcgc gtaactttcg tattcatctg ctgaatgctc tcaggtgagg gaaatttcag
    30121 cgaaaaagcc cgaaaaatgt gctgttaatc acatgcctaa gtaaaaattt gacgacacgt
    30181 attgaagtgc ttcaccatag cctacagatt atttcggagc gcgaaaatat agggagtatg
    30241 cggtggttgc tgaaaaccag cctgggcaca ttgatcaaat aaagcagacc aacgcgggcg
    30301 cggtttatcg cctgattgat cagcttggtc cagtctcgcg tatcgatctt tcccgtctgg
    30361 cgcagctggc tcctgccagt atcactaaaa ttgtccgtga gatgctcgaa gcacacctgg
    30421 tgcgagagct ggaaatcaaa gaagcgggga accgtggccg tccggcggtg gggctggtgg
    30481 ttgaaactga agcctggcac tatctttctc tgcgcattag tcgcggggag attttccttg
    30541 ctctgcgcga tctgagcagc aaactggtgg tggaagagtc gcaggaactg gcgttaaaag
    30601 atgacttgcc attgctggat cgtatcattt cccatatcga tcagtttttt atccgccacc
    30661 agaaaaaact tgagcgtcta acttcgattg ccataaccct gccgggaatt attgatacgg
    30721 aaaatggtat tgtacatcgc atgccgttct acgaggatgt aaaagagatg ccgctcggcg
    30781 aggcgctgga gcagcatacc ggcgtaccgg tttatattca gcatgatatc agcgcatgga
    30841 cgatggcaga ggccttgttt ggtgcctcac gcggggcgcg cgatgtgatt caggtggtta
    30901 tcgatcacaa cgtgggggtg ggcgtcatta ccgatggtca tctgctacac gcaggcagca
    30961 gtagtctcgt ggaaataggc cacacacagg tcgacccata tggtaaacgc tgttattgcg
    31021 ggaaccacgg ctgcctcgaa accatcgcca gtgtggacag tattcttgag ctggcacagc
    31081 tgcgtctcaa tcaatccatg agctcgatgt tacatgggca accgttaacc gtggactcat
    31141 tgtgtcaggc ggcattgcgc ggcgatctac tggcaaaaga catcattatc ggggtgggcg
    31201 cgcatgtagg gcgcattctt gccatcatgg tgaatttatt taacccacaa aaaatactga
    31261 ttggctcacc gttaagtaaa gcggcagata tcctcttccc ggtcatctca gacagcatcc
    31321 gtcagcaggc ccttcctgcg tatagtcagc acattagcgt tgagagtact cagttttcta
    31381 accagggcac gatggcaggc gctgcactgg taaaagacgc gatgtataac ggttctttgt
    31441 tgattcgtct gttgcagggt taacattttt taactgttct accaaaattt gcgctatctc
    31501 aatttgggcc aggaaagcat aacttagact ttcaaggtta attattttcc tagtttatat
    31561 ttgtgaagca taacggtgga gttagtgatg ctgaagcgtt tctttattac cggtacagac
    31621 acttctgtag ggaaaacggt ggtttcccgc gcattgctac aagcgttagc ctcccaggga
    31681 aaaagggttg cgggatataa acccgtagcg aaggggagca aagagacacc cgaagggctg
    31741 cgtaataaag atgccttggt gttgcagagt gtttcaacca tcgaactgcc ttatgaagca
    31801 gttaatccta tcgcgttaag cgaagaagaa agtagcgtgg cgcacagttg cccaatcaat
    31861 tacaccctca tttcaaacgg cctggcaaac ctgaccgata aagtcgatca tgtcgtggta
    31921 gaagggactg gcggctggcg cagtctgatg aatgatttgc gtccactctc tgaatgggta
    31981 gtgcaggaac aactgccggt gttgatggtt gtcggtattc aggaaggttg cattaaccat
    32041 gcactgctaa cagctcaggc gatcgccaac gacgggctgc cgctcattgg ctgggtggct
    32101 aaccgaatca acccaggact ggcgcattat gcggaaatca ttgatgtgct gggtaaaaaa
    32161 cttccggcac cgctcattgg tgaactgcct tatctgccgc gcgccgaaca gcgtgaactg
    32221 gggcaataca tccgcttagc tatgctgcgc agtgtgctgg cggtagatag agtcacggtg
    32281 taacgtccgc gaaattaccg acgcaattac gcaggcaatc aataaaccgg ggagtagctg
    32341 atactccccg gtcatttcac atatcatcaa cgtcgacata atcggcgcgt gcgtggttgc
    32401 cgccaacagt gtcgccatcc cggtcaatcc gagtaaaagc gtaatttctt cgccatcagg
    32461 gaaccataat cccaggctac gaccatacaa catgccaatg gcaagaccga taaatagcgt
    32521 cggtgtaaag acaccaccgg gtgcgccgga accgctactc gccagcacgg cacacagttt
    32581 acaaaggaag atcccggcaa tgatcattaa cagtggtggg gcggttaaaa aggattgtac
    32641 ggtgctatag ccgttgcccc acactgcagg tgtgaacagg gaaagcagac ccacgatcaa
    32701 cccgactagt gccagttgcc agggcggcgc aagtttgaga ctcacaaatc cacgatgaca
    32761 ggcgttcatt aacgttaaca acagtggtcc gcacagacct gccagcacac ctgtactgat
    32821 aatcagcgca tagtcacgag cctgaaccgt cactgagagt tgtacgttgt agagtaacgc
    32881 gtcgctatga ttaatcagat tgctaaccag ccatgccacg atggcggaaa taatcaccgg
    32941 gccgagagag gccaacatca tagtgccaaa cagcacttcg gctataaata aactgccagc
    33001 aagcggggca cgataggccg cagccattcc cgccgcggcc ccacaggcga tccataattt
    33061 ccattcctgg cgtggcgtaa aacgttgggc aaaacaggag gcggcaaggg cagctaaaag
    33121 aatcatcgca ccttcgcgac caattgcact gccgctggtt actaccagca gcgaggcaag
    33181 cgatttaacc aggcttgctg cgtaatcgaa ctgtccatcg gtttgcaacg cttccatgta
    33241 atcggtcggc gcatgagggc gttgttgggt aaatttctgc cagcccatca gcaacaaacc
    33301 cgccgccagt ccgccgagcg ccggagttag cagccgtcgc caggaggaaa ggtttgtcgc
    33361 tgcattgacc agactgccgg agtcattatt gaggaacaac cactccagta gcagcatcgc
    33421 atgacgaaac ccggcaacgg caaagacagc gagaataccg acgactgttg cgataagcag
    33481 acggcggaac atagtgcgca gatccggata gatgtgtaga tgatgcatga gcagaaatgg
    33541 gcaatgaaac acgggcgact attttgcggc aaaagaggcc aaccgcaaaa gcagtgcgtg
    33601 ctttaagaga taaaaaaacg tgacattgta acgtattatt gccgaatcgc gcgtaatcgt
    33661 caccatccgg caatattacg gtgatcctat cgaaagagtg gtttaaccgc gacaggaatc
    33721 aacagttgtg attgccattg cgccagcgtt aagcgagcca gttcacccag tgcacggtag
    33781 aaagggtgtt ctgctttttc gataaaaaca tcaagaaaac gtgttgacca cggaaaaagg
    33841 tgccatgcca gcaattcttc gcattctgtc tggcgaccat tctccgccaa ccacgcagcc
    33901 atcaacagca gtgaaccaaa atgatcttcc ggttcgtttt gcttcatttc aaactgaatg
    33961 cctttctcgc gcatccactg acgaagtgcc aatgttgaat cgccaaacag cacagattcg
    34021 cgatccagcc agaccgaccc ccacggcgga gacggcagtg cccacgggcc gacaaacaaa
    34081 cgctgccagg cctgggcgtg agtctcttca cactgtgtct gaaaagccgt caccagcggc
    34141 gctaatgacg cttctggtag aggccactga gtttcccagc cgtcactggt gagtaccgca
    34201 accagaggtg cggcttccgc actctctgga gcgtaataaa acagcgcgcc caacacgcgc
    34261 gccgcgacag aaaaattatc ttgctgtgaa aaatgggtca ttcctaacat cctgaaatgt
    34321 gcgggttgcc ccgcaccaat ttgttaacct gcaatagcca ttcctactgt catgtgcagg
    34381 ccataaaaga gcacacgccc gatcatctca ccaacaagta tgaggattag ccccagaaca
    34441 agtccggcaa cgtgaggttc acggcgacga atgagcggac aaagccagca tccaagacct
    34501 gcgcataaca ataccacacg ccagacctgt aaggaggcgt aatctggtac cagtgcgctg
    34561 gcttgttgca cggatgagtg aatagacgcc agagacaaac cttgcagcac aatcacctcg
    34621 gcacaagcga tcagcgccag aacgctaatg atggcaaatg gtgtggtatt aaatgtaacg
    34681 cgggctgccc gcagaattgc ggcagcaagt attggaccgc tcaacaatac cgtcaggaag
    34741 aaagccagcg tagtgtaacc gttatgccag gttggcacgg tgtcgatttg atacacgcag
    34801 gtcatcatcc agacgaaaat gacgccgagc gccatgctga caagtaacca gagtttcccc
    34861 aacgcttgcg gcattttacc gatgaccgcc accagccacc atagtccgcc aacggcaaaa
    34921 aatatggaac cggcagcaat ctcgttactc agaccagaag ctccgattcg attcagcgag
    34981 ttaaacgctc gcagtggcga tcccaggtgc atgacagagg caataaaccc gatgcccatc
    35041 aacagccaga ggaaaaacat gccacgaaca atgtgctgac ggtctgcgtc atttttggcg
    35101 gcgaaccagc caattccgct aacaatcagc gcgcctacta cgcattgacc caatacggta
    35161 aagataacca gtggccattc atgccatcca tttcccattt tacacttcct ccggattagc
    35221 cagataacca gtggtatcgc cggtcgggcg gctgttggcg ttaggtttga taacgatatt
    35281 gggttttgtg aaatgcgcgc gcggcagtgg cgcgacggca gccagagtgc cgtgcttctg
    35341 gcgcagctct tcaattggac cgaactcaag tgcacgcagc gggcaggatt cgacgcatat
    35401 gggttgtttc ccctcggcga cgcgcgaata acaaccatcg cacttcgtca tgtgcccttt
    35461 ttcagcattg tactgtggtg cgccgtacgg acaagccatg tggcagtagc ggcagccgat
    35521 acaaacatct tcatcaacca ccacaaagcc atcttcacgc ttatgcattg cgccgctggg
    35581 gcagactttt gtacatgcgg ggtcatcgca atggttacag gagatggaga gataataagc
    35641 gaacacgttc tggtgccaga tgccattatc ttcctgccag tcgccgccag catattcata
    35701 aatacggcgg aaactgactt ccgggccaag atctttaaaa tctttgcacg ccagttcgca
    35761 ggttttacag ccagtacagc ggctggaatc gataaaaaat ccatattgtg tggtcatggg
    35821 ctactcctta aaccttttcg atctggacaa gattgctgtg cgacgggttt ccctttgcca
    35881 gcggtgaagg gcggtgagag gtcagaatat tgatactgcc gccgtgatcg acccggtcac
    35941 caaacatatc cgctttaagc cacgcacctt gcccgatggc ggtaacgcca ggcagaatac
    36001 gcggagtcac ttttgcggca atcagcattt ctccattatt gttaaatacc cgcacggtat
    36061 cgccatgacg gataccgcgt gcctgagcat caatggggtt gatccacacc tcttgtgggc
    36121 aggcctgctg taacacatca atattgccgt agctggagtg ggtacgcgct ttggtttggc
    36181 gacttcactc agcatccagt agatgggttt gcgttcaaat tttgctgagg ttgcgggttg
    36241 ggcgaggatc acatagccca tattccctgc agattcatga gagataaggt cttcttgctc
    36301 tgttggcatc aggtcaggca acaggatatc gcaatactta gccgaggccg tcatgaagtg
    36361 gtcaatgcca acaatcatct cgcacttgct gtcatcctga agcacctcat gggtgtgatt
    36421 gatgtcgcca tgttgattga tcaatgtgtt actggcgtag caccataaaa acttgatggg
    36481 gacatccagt ttttcttttc cacgaacacc atcacgggtc gcggtcattt tcgtaccatg
    36541 gtcgatggca tctgtccatg taaaaacgga aatctgcgtt ttaacaggat tctcgagcat
    36601 cgggaaccat tctaccccca gatcccagct accttcgcgt acacctgagt tgccgccgtt
    36661 tatgccgacg ttaccggtaa gaacggaaag catggcaata gcgcgggacg tttgttcgcc
    36721 gttggagtgt cgttgtggcc cccaaccctg acaaatataa gcaggttttg ctgaaccgat
    36781 ctctcgtgcc aactggataa ttttttctgc cgggatgctg gtgatttttg ctgcccattc
    36841 cggcgtttta gctatgccgt caggcccttc gcccagaata taggctttat aatgcgcgtt
    36901 acgtggtgcg ttggcgggca gcgttttttc atcgtaacca acacaatatt tgtcgagaaa
    36961 tggctgatcg accatgtttt cagtaatcag tacccaggca atcgcacagg ccagtgcgcc
    37021 atcggtgcca gggcgaatgg gcagccattc atcttcacgc ccggcagctg tgtcgttata
    37081 gcgtggatcg atgacgatca tgcgtgcgtt tgaacgttcg cgggcttgct cgacgtagta
    37141 agtgacacca ccgccgctca tccgcgtttc tgccgggtga tttccgaaca taacgaccag
    37201 tttcgtattg gcgatatcat ccgggctgtt gccatcattg gcaccgaaca tataactcat
    37261 tgcggcactg atctgtgcgg tactgtagct gccatagcga ctgagaaaac ccccgcaaga
    37321 gttcatcaga cggtacggga cgttcgagtt ggtgatattt ccgccatcta cgcctgttcc
    37381 gtacaggaca tgtacagcct cattgccgta atctttcagg atccgccgaa gattatcact
    37441 gatggtatcc agggcttcgt cccaacttat ccgttcaaat ttaccttcac cgcgctcgcc
    37501 gacgcgcttc atgggatatt tcaacctgtc aggatgattc atccgtcggc ggatagagcg
    37561 cccgcgtaaa catgcccgaa cctgatgatt accgtaaacg tcgtcacctg tcgtatcaga
    37621 ctccacccag tacacggtgt catctttcac atgcaaacgt agcagacagc ggctcccgca
    37681 gttaacggtg caggaactcc agaccgtttt ctcttctacc ggagcctctg ccgcccggac
    37741 catttgggaa aatggcagag tgaaagcact gcttgccagc gcgagactgc caagtgcgga
    37801 ggttttcatc agacttctat ggctgatttc agccttcatg agcgcctctg tggtatggat
    37861 tttcatcatt actcacttat tgcttttcaa acaaaatgtc atgccagaat ttatggttgt
    37921 agtgggttat attttttcga tctcgaccag attagtgtgc tgcgggtttc ccttcgccag
    37981 tggtgaaggg cgcagagtgg ttagcgtatt cacacatccg ccatggtcga ttttatcgcc
    38041 agacatattg ggctcgtgcc aggctccctg gcccatagcg ctaactccag ggagaatgcg
    38101 tggtgttact ttggctggta gccgaacttc gccacgatgg ttaaacaccc gcaccatatc
    38161 gccgttggca atcccacgtt tctgtgcatc tatagggttg atccacacct cctgacggca
    38221 ggctgccttc aggacatcaa tgttgccgta ggtcgagtga gtacgggatt tgtaatggaa
    38281 accaaacagt tgcagtggga aggttctacg ttcaggggag tcccagcctt caaaggttga
    38341 ggcataaact ggcagtgggc ttatcacttc atctttttcc agttcccagg tacgggcaat
    38401 ttccgccagc ttgctggaat aaatttcaat cttaccggaa ggcgttttaa gtggatttgc
    38461 ctcggggtcg tcacgaaatg ctttgtaggc gacaaaatgg ccattgggat ctttacgctt
    38521 atagataccc atttttttca gttcgtcgta agacggtaac gccggatctt tggcaagcat
    38581 tttggcgtac agatgttgta accattgttc ctgcgtgcga ccttctgtga acttttgata
    38641 gacgtcaggt ccaagacgtt tcgcgacttc actcaggatc cagtaaatcg gtttacgttc
    38701 gaatttttcg ctggtgactg gctggaggaa aatgagatat cccatgttac cggcgtagtc
    38761 gttaggaata atatcttcct gctcaacggt catcaggtct gccagcagaa tgtcggcata
    38821 ttttgccgat gaggtcataa agttttcgat gaccacaatc atttcgcatt tcgattcgtc
    38881 ctgcagaatt tcatgcgttt tgttgatgtc agaatgctga ttaacgaggg tatttcccgc
    38941 gtagttccag atggactcta ctgtcagacg gatggacgga cttgaagtag ttgactcaac
    39001 gctacttaag gtcctgatat gactgacatt aaccatgacg atactgcata tccatccagt
    39061 tctttctctg ggtctcccta tctgtatcag caagtctaca cctcaagtcc tgacacaagg
    39121 ctgacacaaa tcaaggacct tgctacgcca cagctagtcc ggagaaccaa cgggcgttac
    39181 actgtccgct tcagacttaa aggagctacc agcccgtttc tatcagtctc aacccgcagc
    39241 acagaccgga ggaccgctac aatgcgtcag agagagctcg cagccacagc taaggcattc
    39301 ctactggaca atcctgaagt cacactacag gagcttacag cgcacatacg gtccatggct
    39361 gagcagttcc tgactgatgc cagtgatgac tactggaatg gcgtagaggt ggcgacactg
    39421 gttgacgaga agtccaacct cagggaactt gcagcgacaa gagcgttgag tgtggaccag
    39481 cagaggggca ttaagttagc gctggaagtc ctgaccgcag cacagcagag agtcgacact
    39541 ggtgatacct ctggtctgat taagctgatt gatggtggcg taagccagct tactgatgat
    39601 aataaccata ctgattactc aactataggc gactctacgt cgattctgaa gaatgagcag
    39661 ggggatagac cagcagtttt cacacaggaa cgtcagttac tcactccaga gtcacgagaa
    39721 tcatctgtac tttttcagga attggtgaca tcactgctgg ctgagaaggt ccagacactg
    39781 aaacaatcca gctttaagga cttgtcagcg tccc
//
LOCUS       DAJTQY010000027        38609 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.027, whole genome shotgun sequence.
ACCESSION   DAJTQY010000027 DAJTQY010000000
VERSION     DAJTQY010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38609
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.027"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(<1..246)
                     /gene="yefM"
                     /locus_tag="OO349_001489"
     CDS             complement(<1..246)
                     /gene="yefM"
                     /locus_tag="OO349_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HCR5866617.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDII"
     gene            529..570
                     /gene="hisL"
                     /locus_tag="OO349_001490"
     CDS             529..570
                     /gene="hisL"
                     /locus_tag="OO349_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HCR5866618.1"
                     /translation="MTRVQFKHHHHPD"
     misc_feature    546..669
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            716..1615
                     /gene="hisG"
                     /locus_tag="OO349_001491"
     CDS             716..1615
                     /gene="hisG"
                     /locus_tag="OO349_001491"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HCR5866619.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPCLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            1621..2925
                     /gene="hisD"
                     /locus_tag="OO349_001492"
     CDS             1621..2925
                     /gene="hisD"
                     /locus_tag="OO349_001492"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HCR5866620.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     TRGDEALREYSAKFDKTTVTALKVSADEIAAASERLSDELKQAMAVAVKSIETFHTAQ
                     KLPPVDVETQLGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCDVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETTRQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNVLKEQA"
     gene            2922..3992
                     /gene="hisC"
                     /locus_tag="OO349_001493"
     CDS             2922..3992
                     /gene="hisC"
                     /locus_tag="OO349_001493"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HCR5866621.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FKLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLENWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIPPYPLSTPVADIAAQALSPQGIVAMSERVAQIITER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            3992..5059
                     /gene="hisB"
                     /locus_tag="OO349_001494"
     CDS             3992..5059
                     /gene="hisB"
                     /locus_tag="OO349_001494"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HCR5866622.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVEGYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            5059..5649
                     /gene="hisH"
                     /locus_tag="OO349_001495"
     CDS             5059..5649
                     /gene="hisH"
                     /locus_tag="OO349_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HCR5866623.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDIVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            5649..6386
                     /gene="hisA"
                     /locus_tag="OO349_001496"
     CDS             5649..6386
                     /gene="hisA"
                     /locus_tag="OO349_001496"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HCR5866624.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPDVVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            6368..7144
                     /gene="hisF"
                     /locus_tag="OO349_001497"
     CDS             6368..7144
                     /gene="hisF"
                     /locus_tag="OO349_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HCR5866625.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVEEVQKRGAGEIVLNMMNQDGVCNGYDLKQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            7138..7749
                     /locus_tag="OO349_001498"
     CDS             7138..7749
                     /locus_tag="OO349_001498"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HCR5866626.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGNTAHQWLFLYQLEQLLAERKYADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLHKRHQ"
     gene            complement(7935..8912)
                     /gene="wzzB"
                     /locus_tag="OO349_001499"
     CDS             complement(7935..8912)
                     /gene="wzzB"
                     /locus_tag="OO349_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HCR5866627.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIEKLKFDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(9058..10178)
                     /gene="ugd"
                     /locus_tag="OO349_001500"
                     /pseudo
     CDS             complement(9058..10178)
                     /gene="ugd"
                     /locus_tag="OO349_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
     gene            complement(10428..11834)
                     /gene="gndA"
                     /locus_tag="OO349_001501"
     CDS             complement(10428..11834)
                     /gene="gndA"
                     /locus_tag="OO349_001501"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HCR5866628.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLAPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRSALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(11943..12302)
                     /locus_tag="OO349_001502"
     CDS             complement(11943..12302)
                     /locus_tag="OO349_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001313975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5866629.1"
                     /translation="MNFNSRLESRYSYELMKKASEYSELYGDNLIQLGLEDGIYFYKG
                     MAIGDVFGLARYSDWTISNPECEVIPQDDLIEKMKSFNSSFIVISKRSYANFNPEKYP
                     KFKVLMDTPNGILIAIK"
     gene            complement(12299..12805)
                     /locus_tag="OO349_001503"
     CDS             complement(12299..12805)
                     /locus_tag="OO349_001503"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5866630.1"
                     /translation="MATILGGIYWYVRSFWISGDPFSPAGGNIFGHYLWNEIDLQVQT
                     AEQARHGISPATLDLQQAINKVGSDVVFYAILSVFTLRNNKILWVFFSIVLTYVLFWF
                     SVTQGDRYLSPIYPLSVLQVAISIASLIKDINITNYKLGRYFILVCIIFFRGDRCILN
                     SNIFILNT"
     gene            complement(12951..13514)
                     /locus_tag="OO349_001504"
     CDS             complement(12951..13514)
                     /locus_tag="OO349_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5866631.1"
                     /translation="MVGQFNKIAIFLIFTVCWVLSIIKRQQFRWLAINNIEFSTLFVI
                     LFLVLIFVVTLLSSLRAPGDWDDTMYHLPLARSLVEHHAIVVEQYLRFPLFPQNADLL
                     MALGLQLGDVRLAQFLANICFFVIACGLVGCSWEITKTYYPGIIATILLFTINPLKDH
                     LGYAYIDLTLSLFCCSQYSYIYSLRKQ"
     gene            complement(13721..14659)
                     /locus_tag="OO349_001505"
                     /pseudo
     CDS             complement(13721..14659)
                     /locus_tag="OO349_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274881.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(14722..15096)
                     /locus_tag="OO349_001506"
     CDS             complement(14722..15096)
                     /locus_tag="OO349_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bactoprenol-linked glucose translocase"
                     /protein_id="HCR5866632.1"
                     /translation="MLKIGKLLTSSFFSYFLIGIVNTALHWGVFYACYNNLAFGQGRS
                     NIVGFICAATFSFFANARCSFKVSATKARYFIFIFFMGAMSYLFGVLFDLLALSPIFT
                     LFTFSLFSLVLGYCASKYFIFR"
     gene            complement(15100..16248)
                     /gene="wzy"
                     /locus_tag="OO349_001507"
     CDS             complement(15100..16248)
                     /gene="wzy"
                     /locus_tag="OO349_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen polymerase"
                     /protein_id="HCR5866633.1"
                     /translation="MNNINKIFITFLCIELIIGGGGRLLEPLGIFPLRYLLFVFSFIL
                     LIFNLVTFNFSITQKCVSLFIWLLLFPFYGFFVGLLAGNKINDILFDVQPYLFMLSLI
                     YLFTLRYTLKVFSCEIFIKIVNAFALYGSLLYISYIILLNFGLLNFNLIYEHLSLTSE
                     FFFRPDGAFFSKSFYFFGVGAIISFVDKKYLKCLIIVLAILLTESRGVLLFTTLSLLL
                     ASFKLHKLYLNTIIIILGSVLFIIMLYMVGSRSEDSDSVRFNDLYFYYKNVDLATFLF
                     GRGFGSFILDRLRIEIVPLEILQKTGVIGVFISLVPMLLIFLKGYFLNSTKTSLMMSL
                     ILFFSITVSITNPFLFTPMGIFIIGVVVLWVFSIENIQISNNLTSGAK"
     gene            complement(16268..17173)
                     /gene="rfbG"
                     /locus_tag="OO349_001508"
     CDS             complement(16268..17173)
                     /gene="rfbG"
                     /locus_tag="OO349_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-rhamnosyl transferase RfbG"
                     /protein_id="HCR5866634.1"
                     /translation="MHSSDQKRVAVLMATYNGECWIEEQLKSIIEQKDVDISIFISDD
                     LSTDNTLNICEEFQLSYPSIINILPSVNKFGGAGKNFYRLIKDVDLENYDYICFSDQD
                     DIWYKDKIKNAIDCLVFNNANCYSSNVIAYYPSGRKNLVDKAQSQTQFDYFFEAAGPG
                     CTYVIKKETLIEFKKFIINNKNAAQDICLHDWFLYSFARTRNYSWYIDRKPTMLYRQH
                     ENNQVGANISFKAKYKRLGLVRNKWYRKEVTKIANALADDSFVNNQLGKGYIGNLILA
                     LSFWKLRRKKADKIYILLMLILNIF"
     gene            complement(17166..18056)
                     /locus_tag="OO349_001509"
     CDS             complement(17166..18056)
                     /locus_tag="OO349_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnosyltransferase"
                     /protein_id="HCR5866635.1"
                     /translation="MNSNIYAVIVTYNPELKNLNALITELKEQNCYVVVVDNRTNFTL
                     KDKLADIEKVHLICLGRNEGIAKAQNIGIRYSLEKGAEKIIFFDQDSRIRNEFIKKLS
                     CYMDNENAKIAGPVFIDRDKSHYYPICNIKKNGLREKIHVTEGQTPFKSSVTISSGTM
                     VSKEVFEIVGMMDEELFIDYVDTEWCLRCLNYGILVHIIPDIEMVHAIGDKSVKICGI
                     NIPIHSPVRRYYRVRNAFLLLRKNHVPLLLSIREVVFSLIHTTLIIATQKNKIEYMKK
                     HILATLDGIRGITGGGRYNA"
     gene            complement(18086..19342)
                     /gene="wzx"
                     /locus_tag="OO349_001510"
     CDS             complement(18086..19342)
                     /gene="wzx"
                     /locus_tag="OO349_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen flippase"
                     /protein_id="HCR5866636.1"
                     /translation="MSIIKNSVWNLFGYAIPTLIAIPSLGFLARGLGPEGFGIYTIAI
                     ALVGYAGIFDVGLTRSVIREIAIHRDNHHERTKVISTSTSFLVLFSCFGAFLLLIFSD
                     GIVNYLKISGVEHSDIQLAFKLLAICIPLFILNQLWSAILEGDEKFGIVNIQKSISSS
                     CIAGIPAIFVFYSATLSAAVAGLIFARVISILVSAYYVRNDIKISGVHFCYKTFKRLF
                     FFGGWMTVSNIISPVMVYFDRFIVSNIMGADKVAFYSAPAEVILKLGIIPAAIGRAVF
                     PRLSNIKDFKEFKRNVNKSLLLMFLICLPVIIIGLLYSGLVLKIWFGENYKINSFNIL
                     NVLLIGFFFNALAMIPFSAIQALGKSKITALIHCAELVPYLALLYFMVEKYGLLGAAI
                     SWSIRVILDALLLQWLYTRMCSVYEN"
     gene            complement(19358..19903)
                     /gene="rfbC"
                     /locus_tag="OO349_001511"
     CDS             complement(19358..19903)
                     /gene="rfbC"
                     /locus_tag="OO349_001511"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HCR5866637.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQKVFEEAVGRKVE
                     FVQDNHSKSTKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFCVLSDEAEFVYKTNNFYSKMQERGILWSDKSINIEWPVQ
                     NPLLSDKDINGQKFVDADYFI"
     gene            complement(19908..20786)
                     /gene="rfbA"
                     /locus_tag="OO349_001512"
     CDS             complement(19908..20786)
                     /gene="rfbA"
                     /locus_tag="OO349_001512"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCR5866638.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDTAVNRESGATVFAYHVNDPERYGVVEFDDNGTAISL
                     EEKPLEPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMDQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            complement(20845..21744)
                     /gene="rfbD"
                     /locus_tag="OO349_001513"
     CDS             complement(20845..21744)
                     /gene="rfbD"
                     /locus_tag="OO349_001513"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HCR5866639.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPNFAQLLNATCVEAIAKAANEVGAWVIHY
                     STDYVFPGNGDTPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVAANKPEVAGLYHLVAG
                     GTTTWHDYAALVFEEARRAGINLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            complement(21744..22829)
                     /gene="rfbB"
                     /locus_tag="OO349_001514"
     CDS             complement(21744..22829)
                     /gene="rfbB"
                     /locus_tag="OO349_001514"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCR5866640.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYAFEHADICDAVAMSRIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALNDEKKKSFRFHHISTDEVYGDLPHPDEANNNEALPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGNQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDADKISRELGWKPQETFESGIRKTVEWY
                     LANTNWVENVKSGAYQSWIEQNYEGRQ"
     gene            complement(23202..24095)
                     /gene="galF"
                     /locus_tag="OO349_001515"
     CDS             complement(23202..24095)
                     /gene="galF"
                     /locus_tag="OO349_001515"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HCR5866641.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVIIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            complement(24270..25664)
                     /gene="wcaM"
                     /locus_tag="OO349_001516"
     CDS             complement(24270..25664)
                     /gene="wcaM"
                     /locus_tag="OO349_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HCR5866642.1"
                     /translation="MPFKTLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGGEGGGMHNITLDVRCSDCTIKGLAMSGFGPVMQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIN
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVSLTNIEMKRASLELHNKPQHLFMRNINV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            complement(25675..26895)
                     /gene="wcaL"
                     /locus_tag="OO349_001517"
     CDS             complement(25675..26895)
                     /gene="wcaL"
                     /locus_tag="OO349_001517"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HCR5866643.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTEN
                     THAAWTKYNLVARTRWLQDEPAGKVAKLRHRASQTLRGIHRKNTWHALNLKRYGAESR
                     NLILSAICGQVATPFRADVFIAHFGPAGVTAAKLRELGIIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGLWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKEMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLLPENDARALAQRLAAFSQLDTDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            complement(26892..28172)
                     /gene="wcaK"
                     /locus_tag="OO349_001518"
     CDS             complement(26892..28172)
                     /gene="wcaK"
                     /locus_tag="OO349_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HCR5866644.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHTEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMTQHNSAAGVVGRIKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRRNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPVLNARFNEAVSRERQTGMQMVQSVLERIGEVK"
     gene            complement(28352..29830)
                     /gene="wzxC"
                     /locus_tag="OO349_001519"
     CDS             complement(28352..29830)
                     /gene="wzxC"
                     /locus_tag="OO349_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HCR5866645.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDVI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYCPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSIVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCIVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            complement(29832..31226)
                     /gene="wcaJ"
                     /locus_tag="OO349_001520"
     CDS             complement(29832..31226)
                     /gene="wcaJ"
                     /locus_tag="OO349_001520"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HCR5866646.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVVGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            complement(31281..32651)
                     /gene="cpsG"
                     /locus_tag="OO349_001521"
                     /gene_synonym="manB"
     CDS             complement(31281..32651)
                     /gene="cpsG"
                     /locus_tag="OO349_001521"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707943.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HCR5866647.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARTISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTIDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRSDGISMTFADWCFNLRTSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            complement(32844..34280)
                     /gene="cpsB"
                     /locus_tag="OO349_001522"
     CDS             complement(32844..34280)
                     /gene="cpsB"
                     /locus_tag="OO349_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HCR5866648.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSVGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMEPTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(34283..35506)
                     /gene="wcaI"
                     /locus_tag="OO349_001523"
     CDS             complement(34283..35506)
                     /gene="wcaI"
                     /locus_tag="OO349_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HCR5866649.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAADNVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADLLRDEPLIFAIVGQSGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            complement(35503..35982)
                     /gene="gmm"
                     /locus_tag="OO349_001524"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(35503..35982)
                     /gene="gmm"
                     /locus_tag="OO349_001524"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HCR5866650.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            complement(35982..36940)
                     /gene="fcl"
                     /locus_tag="OO349_001525"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /pseudo
     CDS             complement(35982..36940)
                     /gene="fcl"
                     /locus_tag="OO349_001525"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707947.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
     gene            complement(36943..38064)
                     /gene="gmd"
                     /locus_tag="OO349_001526"
     CDS             complement(36943..38064)
                     /gene="gmd"
                     /locus_tag="OO349_001526"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HCR5866651.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(38090..>38609)
                     /gene="wcaF"
                     /locus_tag="OO349_001527"
     CDS             complement(38090..>38609)
                     /gene="wcaF"
                     /locus_tag="OO349_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HCR5866652.1"
                     /translation="KGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRAFLLRLFGAKI
                     GKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQKSYLCTGSHD
                     HASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKSLPANVVCRGN
                     PAVVIRERVETE"
BASE COUNT        10318 a         9672 c         8687 g         9932 t
ORIGIN      
        1 aataatgtcc ttttccgttc ctttgcctga tttcaggcta tcgattgagt ccatcaatct
       61 ccgggcgtta gcgggggaac gcagtagata agccgtctct tccagcgaat tgtattcttc
      121 gagtgacatc agaacacaag cctctccatt ctgacgagta atgaggatcg gggcatgatc
      181 ttcaacggct ttcatcattg ttgccgacaa attctgacgc gcttcgctgt agctaattgt
      241 acgcatgtaa atctcctatt ttgtacagtt cattgtacaa tgataggtgt taattaacta
      301 tttattaatt agtttgtaga tcaagacatt gccagcgaga cgaaaaatca gactttgctc
      361 tttttggtgc tgtcaagtta gaggacagtc ctcttagccc cctcctttcc ccgctcattc
      421 attaaacaaa tccatcgcca taaaatatat aaaaaagccc ttgctttcta acgtgaaagt
      481 ggtttaggtt aaaagacatc agttgaataa acattcacag agatttttat gacacgcgtt
      541 caatttaaac accaccatca tcctgactag tctttcaggc gatgtgtgct ggaagacatt
      601 cagatcttcc agtggtgcat gaacgcatga gaaagccccc ggaagatcac cttccggggg
      661 cttttttatt gcgcggttgt taacggttca gacaggttta aagaggaata agaaaatgac
      721 agacaacact cgtttacgca tagctatgca gaaatccggc cgtttaagtg atgactcacg
      781 cgaattactg gcgcgctgtg gcattaaaat caatcttcac acccagcgcc tgatcgcgat
      841 ggcagaaaac atgccgattg atattctgcg cgtgcgtgac gatgacattc cctgtctggt
      901 aatggacggc gtggtagacc ttgggattat cggcgaaaac gtgctggaag aagagctgct
      961 taaccgccgc gcccagggtg aagatccgcg ctactttacc ctgcgtcgtc tggatttcgg
     1021 cggctgccgc ctttcgctgg caacgccggt tgatgaagcc tgggacggtc cgctctcctt
     1081 aaacggtaaa cgtatcgcca cctcttatcc tcacctgctc aagcgttatc tcgaccagaa
     1141 aggtatctct tttaaatcct gcttactgaa cggttctgtc gaagttgcgc cgcgtgccgg
     1201 gctggcggat gcgatttgcg atctggtttc caccggtgcc acgctggaag ctaacggcct
     1261 gcgcgaagtc gaagttatct accgctcgaa agcctgcctg atccagcgcg atggcgaaat
     1321 ggaagaatct aaacagcaac tgatcgacaa gctactgacc cgtattcagg gtgtgatcca
     1381 ggcgcgcgaa tcaaaataca tcatgatgca cgcaccgacc gaacgcctgg atgaagtcat
     1441 cgccctgctg ccaggtgccg aacgcccaac cattctgccg ctggcgggtg atcaacagcg
     1501 cgtagcgatg cacatggtga gtagcgaaac cctgttctgg gaaacgatgg aaaaactgaa
     1561 agcactgggt gccagttcaa tcctggtcct gccgattgag aagatgatgg agtgatcgcc
     1621 atgagcttta acacaatcat tgactggaat agctgtactg cggagcaaca acgccagctg
     1681 ttaatgcgcc cggcgatctc cgcttctgaa agcattaccc gcaccgttaa cgatattctc
     1741 gataacgtga aaacgcgtgg cgatgaggcc ctgcgtgaat acagcgcgaa gtttgataaa
     1801 accacggtta ccgcgctgaa ggtgtctgct gatgagatcg ccgccgccag cgaacgcctg
     1861 agcgacgagc taaaacaggc gatggcggtg gcagtaaaga gtattgaaac cttccacact
     1921 gcgcaaaaac tgccgccggt agatgtagaa acgcagctag gcgtgcgttg ccagcaggtc
     1981 acgcgcccgg tggcttcagt gggtttgtat attccgggcg gctccgcccc gctcttctca
     2041 acggtattaa tgctggcaac tccggcgcgt attgcgggct gtaaaaaagt ggtgctgtgc
     2101 tcaccgccgc cgattgccga tgaaattctt tatgcggcgc agctgtgcga tgtgcaggac
     2161 gtgtttaacg tcggcggcgc acaggccatt gccgcgctgg cgtttggtac ggaatctgtg
     2221 ccgaaagtgg acaaaatctt cgggccgggt aacgcctttg tcaccgaagc aaaacgccag
     2281 gtaagccagc gtctggacgg tgcggcgatc gatatgcccg caggcccgtc ggaagtgctg
     2341 gtgattgctg acagcggcgc tacgccggat tttgtggctt ctgatttgct ttctcaggct
     2401 gaacacggcc ctgactcaca ggtaatttta ctgacccccg atgctgatat ggcgcgtcgc
     2461 gttgccgagg ccgtcgaacg ccaactggca gaactgccgc gtgccgaaac cacccgccag
     2521 gcactgaacg ccagccgcct gatcgtgact aaagatttag cgcagtgcgt ggagatctcc
     2581 aaccagtacg gcccggagca cctgatcatt cagacccgca acgcccgcga actggtcgat
     2641 ggcatcacca gcgccggttc ggtatttctt ggtgactggt caccggaatc ggcaggcgac
     2701 tacgcctccg gcaccaacca cgtgctgccg acttacggtt acaccgccac ctgttccagc
     2761 ctcgggctgg cagatttcca gaaacgcatg accgtacagg aactgtcgaa agaaggtttc
     2821 tccgcgctgg cttcgaccat tgaaacactg gccgccgccg agcgcctgac cgcccacaaa
     2881 aatgccgtta ctttgcgtgt taacgtcctt aaggagcaag catgagcacc gtgactatta
     2941 ctgatttagc gcgtgaaaac gtccgcaacc tgacaccgta tcagtcggcg cgtcgtctgg
     3001 gcggtaacgg cgacgtctgg ctgaacgcca acgaatatcc cacagccgtg gagtttaagc
     3061 ttactcagca aacgctcaac cgctacccgg aatgccagcc aaaagcggtg attgaaaatt
     3121 acgcgcaata tgcaggcgta aaaccggaac aggtgctggt cagccgtggc gcggacgaag
     3181 gtattgaact gctgatccgc gctttttgcg aaccgggtaa agacgccatc ctctactgcc
     3241 cgccaacgta cggcatgtac agcgtcagcg ctgaaaccat tggtgtcgaa tgccgcacag
     3301 tgccgacgct ggaaaactgg caactggact tacagggcat ttccgacaag ctggacggcg
     3361 taaaagtggt ctatgtttgc agccccaaca accccaccgg gcaactgatc aatccgcagg
     3421 attttcgcac cctgctggag ctaacccgcg gtaaggcgat tgtggttgcc gatgaagctt
     3481 atatcgagtt ttgcccgcag gcatcgttag ctggctggct ggcggaatat ccgcacctgg
     3541 cgattttgcg taccctttcg aaagccttcg cgttggcggg gcttcgttgc ggatttacgc
     3601 tggcaaacga agaagtcatc aacctgctga tgaaagtgat ccccccctac ccgctctcga
     3661 cgccggttgc cgacattgcc gcacaggcgt taagcccgca gggaatcgtc gccatgagcg
     3721 aacgggtggc gcaaattatt acagaacgcg aatacctgat tgccgcactg aaagagatcc
     3781 cctgcgtgga gcaggttttc gactctgaaa ccaactacat tctggcgcgc tttaaagcct
     3841 ccagtgcagt gtttaaatct ttgtgggatc agggcattat cttacgtgat cagaataaac
     3901 aaccctcttt aagcggctgc ctgcgaatta ccgtcggaac ccgtgaagaa agccagcgcg
     3961 tcattgacgc cttacgtgcg gagcaagttt gatgagtcag aagtatcttt ttatcgatcg
     4021 cgatggaacc ctgattagcg aaccgccgag tgattttcag gtggaccgtt ttgacaaact
     4081 cgcctttgaa ccgggcgtga tcccggaatt gctgaagctg caaaaagcgg gctacaagct
     4141 ggtgatgatc actaatcagg atggtctggg aacacaaagt ttcccgcaag cggattttga
     4201 tggcccgcac aacctgatga tgcagatctt cacctcgcaa ggcgtgcagt ttgatgaagt
     4261 gctgatttgt ccgcacctgc ccgccgatga gtgcgactgc cgtaagccga aagtaaaact
     4321 ggtggagggt tatctggctg agcaagcgat ggatcgtgcc aacagttatg taattggcga
     4381 tcgcgcgacc gacattcaac tggcggaaaa catgggcatt aatggtttac gctacgaccg
     4441 cgaaaccctg aactggccaa tgattggcga gcaactcact aaacgagacc gttacgccca
     4501 tgtagtgcgc aacaccaaag agacgcagat tgacgtccag gtgtggctgg atcgcgaagg
     4561 tggcagcaag attaacaccg gcgttggctt ctttgatcac atgctggatc agatcgccac
     4621 ccacggcggt ttccgcatgg aaatcaatgt caaaggcgac ctctatatcg acgatcacca
     4681 caccgtcgaa gataccggcc tggcgctggg cgaagcgtta aaaattgccc tcggcgacaa
     4741 acgcggtatt tgccgctttg gttttgtgct gccgatggac gaatgccttg cccgctgcgc
     4801 actggatatc tctggtcgcc cgcacctgga atataaagcc gagtttacct accagcgtgt
     4861 gggcgatctc agcactgaaa tgatcgaaca cttcttccgt tcgctctctt acaccatggg
     4921 cgtgacacta cacctgaaaa ccaaaggtaa aaacgatcat caccgtgtag aaagcctgtt
     4981 caaagccttt ggtcgtaccc tgcgccaggc catccgcgtc gaaggcgaca ccctgccctc
     5041 gtcgaaagga gtgctgtaat gaacgtggtg atccttgata ctggctgcgc caacctgaac
     5101 tctgtgaagt ctgccattgc gcgccacggt tatgaaccca aagtcagccg tgacccggac
     5161 attgtgttgc tggccgataa actgttttta cccggcgtcg gcaccgcaca agcagcgatg
     5221 gatcaggtgc gtgagcgcga gctgtttgat ctcatcaaag cctgtaccca accggtgctg
     5281 ggcatctgct tagggatgca actcctggga cgacgcagcg aagagagcaa tggcgtcgac
     5341 ttgctgggca tcatcgacga agacgtgccg aaaatgaccg actttggtct gccactgcca
     5401 catatgggct ggaaccgcgt ttatccgcag gcaggcaacc gcctgtttca ggggattgaa
     5461 gacggcgcgt acttttactt tgttcacagc tacgcaatgc cagtcaatcc gtggaccatc
     5521 gcccagtgta attacggcga accgttcacc gcggcggtac aaaaagataa cttctacggc
     5581 gtgcagttcc acccggagcg ttctggtgcc gctggcgcta agttgctgaa aaacttcctg
     5641 gagatgtgat gattattccg gcattagatt taatcgacgg caccgtggtg cgtctccatc
     5701 agggcgatta cggcaaacaa cgcgattacg gtaacgaccc gctgccgcgc ttacaggatt
     5761 acgctgcgca aggtgccgaa gtgttgcacc tggtggatct gaccggggca aaagatccgg
     5821 ctaagcgtca aatcccgctg attaaaaccc tggtcgcggg cgttaacgtc ccggtgcagg
     5881 ttggcggcgg tgttcgtagt gaagaagacg tggcggcgtt actggaagct ggcgttgcgc
     5941 gcgtggtggt cggttccacc gcagtgaaat caccggacgt agtgaaaggc tggtttgaac
     6001 gtttcggtgc cgatgcgctg gtgctggcac tggatgtccg tattgacgag caaggcaaca
     6061 agcaagtggc agtcagcggc tggcaagaga actcgggcgt ttcactggaa caactggtgg
     6121 aaacctatct gcccgtcggc ctgaaacatg tgttgtgtac cgatatctcg cgcgacggca
     6181 cgctggcggg atctaacgtc tctttatatg aagaagtgtg tgccagatat ccgcaagtgg
     6241 cgtttcagtc ctccggcggc attggcgaca ttaacgatgt tgctgcccta cgtggcactg
     6301 gcgtgcgcgg cgtaatagtt ggtcgtgcat tactggaagg taaattcacc gtgaaggagg
     6361 ccatcgcatg ctggcaaaac gcataatccc gtgtctcgac gttcgtgatg gtcaggtggt
     6421 gaaaggcgta cagtttcgca accatgaaat cattggcgat atcgtgccac tggcaaaacg
     6481 ctacgccgaa gaaggtgcag atgaactggt gttctacgat atcaccgctt ccagcgatgg
     6541 ccgtgtggta gataaaagct gggtatctcg cgtggcggaa gtgatcgaca ttccgttttg
     6601 tgtggcgggt gggattaagt ctctggaaga tgccgcgaaa attctttcct ttggcgcgga
     6661 taaaatttcc atcaactccc ctgcgctggc agacccaaca ttaattactc gcctagccga
     6721 tcgctttggc gtgcagtgta ttgtggttgg tattgatacc tggtacgacg ccgaaaccgg
     6781 taaatatcat gtaaatcaat ataccggcga tgaaagccgc acccgcgtca ctcaatggga
     6841 aacgctcgac tgggtagagg aggtgcaaaa acgcggcgcc ggagaaatcg tcctcaacat
     6901 gatgaatcag gacggcgtgt gtaacggtta cgacctcaag cagctgaaaa aagtgcgtga
     6961 agtttgccac gtcccgctga ttgcctccgg tggcgcgggc accatggaac acttcctgga
     7021 agccttccgc gatgccgacg ttgacggtgc gctggcagct tccgtattcc acaaacaaat
     7081 aatcaatatt ggcgaattaa aagcgtacct ggcaacacag ggcgtggaga tcaggatatg
     7141 ttaacagaac aacaacgtcg cgaactggac tgggaaaaaa ccgacggtct gatgccggtg
     7201 attgtgcaac acgcggtatc tggcgaagtg ttaatgctgg gctacatgaa cccggaagcc
     7261 ttagacaaaa ccatcgaaag cggtaaagtc accttcttct cgcgcactaa acagcgactg
     7321 tggaccaaag gcgaaacctc gggcaatttt ctcaatgtgg taagtattgc cccggactgc
     7381 gacaacgaca cattactggt gctggcgaat cccatcgggc caacctgcca caaaggcacc
     7441 agtagctgct tcggcaacac cgctcaccag tggctgttcc tgtatcaact ggaacaactg
     7501 ctcgccgaac gtaaatatgc cgacccggaa acctcctaca ccgccaaact gtatgccagc
     7561 ggcactaaac gcattgcgca gaaagtgggc gaagaaggcg tggaaaccgc actggcagca
     7621 acggtacatg atcgctttga gctgaccaac gaggcatctg acttgatgta tcacctgctg
     7681 gtgctgttgc aggatcagga tctggattta acgacagtaa ttgagaacct gcataaacgg
     7741 catcagtgag ttacgggctg agcaggtgtg atgttgttgt cacacccgga gccattgccc
     7801 gatgcgcaaa cttatcgtgc ctacacatta tctcaacaca cgtaggccgg ataagatgcg
     7861 ccagcatcgc atccggcaaa aaaaacgggc atggtgtcac caccctgccc gtttctctta
     7921 aatgcacaat aatattactt cgcgttgtaa ttacgcagag cgttacgccc caacacaatc
     7981 cccgcgccaa ccatgccgcc cagaagcact gccagaatca aggtgattgc ctttttcgga
     8041 ctatcgcgac gaataggtaa cgtcggtttc atcacatagc ggtaagcatg aatatcaaga
     8101 tcatcaaact ttaatttttc aatatccaac aggttttgac gtgtctgata gtagtttggt
     8161 gagaacacca acggacgagt cgcttcatgc ttaatcatcg actccagcgc ttcgctccct
     8221 agaaggaaca acgtatcttg cgtcacatct tcagtctgct gaacctgtgg ctttgtcacc
     8281 tgcgcctgat tcgcatactg caacgcttcc tgaatctgac ggatacgcag atctttctgc
     8341 tcctgcgcga ccacttcctg ggttcttaaa gagtcctgca agtttttccg tcccagagca
     8401 atgttgtcct tgagatcctt ttctagctct tgattcactt tatcatcaac ttgctgaatg
     8461 tattgggcca acttcatttg tgcgccctct gcagtttgcc caacataaga aacagtcaat
     8521 ggtaattgct ggttcttaac agaaggttcg atggtaagtt tttctggctc ttcctgatta
     8581 tccagcgttt ctgctaatgc agagaaggca gaactgaagc gaccaattaa cgtctcctgc
     8641 aaatccgata ctttcggtgc agcctgacca tagataacat tcatggcatt gttatagcca
     8701 gcaatttgcc ccacgtcggg ctgagtgata attgctgttg acgtccattt ctccttcgct
     8761 actgccaaat aaccaatagc cagggcaata gccacaatga cggaaatgat aattgtcatc
     8821 ttgccacgcc acaactgcac tagtaaatca atcaaatcaa tctgttccgg gtcatggttt
     8881 tgcccagaaa cattattatt ttctactctc atcattaccc taactgacgg aaatttccaa
     8941 gtccgcatag tttagcgata atccccgtga atgctatagg ataagtgata aaaataatga
     9001 attattgcgg aggtaacaaa agacaatcag ggcgtaaata gccctgataa caagatgtta
     9061 gtcgctgcca aagagatcgc gggtgtagac cttatctgcc acatccttaa gctcttctgc
     9121 catacggttg gagataatga cgtcggcttg ttgtttgaag gtggcgagat cacgttccag
     9181 gcgagagttg aagaatgagt cttctttcat caccggctcg tagatgatca cttcaacgcc
     9241 tttcgccttg atacgcttca taatcccctg aatagaagac gcacggaagt tatctgaccc
     9301 gctcttcata atcagacgat aaatacccac cacttgcggt ttgcatcgac aattgccgag
     9361 atcaggttat tcggcacaga ctggtagttc gccagtaact gcttggtatc tttcggcaga
     9421 caataaccac cataaccaaa cgacggattg ttgtaatggt tgccaatacg cggatcgaga
     9481 caaacgcctt cgataatctg gcgagtattc agacctaaac tttctgcata actatccagt
     9541 tcattaaagt atgctacgcg catcgccaga taggtattag cgaaaagttt aatcgcttct
     9601 gcttcagtgg agtcggtaaa cagggtcggg atattttgct taatcgcgcc ttcctgtaac
     9661 aacgcagcaa aacgttctgc gcgttctgaa cgctcaccga tgacaatacg tgacggatgg
     9721 agattatcgt aaagggcttt accttcgcgg aggaattccg gagagaaaat aatattttca
     9781 gtacgatatt tcttatgcat cgctgcggta aaaccaacgg gaaccgttga tttgatgacc
     9841 ataaccgcat aaggatttat ctcaactacg tctttaatta ctgattctac actggatgta
     9901 ttgaaataat tagttttagg atcatagtcg gttggagtgg cgatgatgac ataatcagca
     9961 tcccggtagg cttcattttt atctaatgtg gcattaaagt gtattttatc tgattgcaaa
    10021 aactgctgaa tttccttatc aacaatagga gatatccgat cattcagcat agcaacgcgt
    10081 gacggtaaaa tatctaatgc cacaacctca tgattttgtg cgattagaag cccgtttgac
    10141 aagcctacat agccagtacc ggaaatggtg attttcattt catgctctca gaattaactt
    10201 aactgtgaat catgatgttt ttagcatcgt gataagatgc taaaagtttt aacgctacgt
    10261 tgtcagatgg tcaacgcaac gaaaaacaat tcagattttc tttataagaa tagctgatgt
    10321 ttattaagtt aatattaagc agtgaaattt agtctataag atatttggca aaaaaaagcc
    10381 cggtgcaata tacgccgggc ctcaatttta ttgttggtta aatcagatta atccagccat
    10441 tcagtatgga acacaccttc tttatcaatg cgcttataag tatgcgcccc gaaatagtca
    10501 cgctgtgcct ggatcaggtt cgcaggcaga accgcggcac ggtagctatc gtaataggca
    10561 accgcagcgg cgaaggtcgg aaccgggata ccgttctgta ctgcataagc gacgacatcg
    10621 cgcagcgcct gctggtagtc atcggcaatt tgcttgaagt acggggccag cagcaggtta
    10681 gcgatctgcg gattttcggc ataagcatcg gtgattttct gcaggaactg cgcacggatg
    10741 atgcagccag cacggaaaat cttcgcgatt tcgccgtagt tcagatccca attgtattct
    10801 tcagacgcag cacgcagctg agagaagccc tgagcgtaag aaacgatttt gcccagatac
    10861 agcgcactgc gaactttttc gatgaactca gccttgtcgc ctgctgactg agcttgcgga
    10921 ccagagagaa ctttagatgc ggcaacgcgc tgatctttca gagaagagat ataacgtgca
    10981 aacacagact cggtaatcag cgacagcggt tcgccgagat ccagcgcgct ctggctggtc
    11041 catttaccgg tgcctttgtt tgctgcttca tccagaatca catcaaccag gtagttaccg
    11101 tcttcatctt ttttggtgaa gatatcttta gtgatgtcga tcaggtagct gctaagttca
    11161 ccgttattcc actcggtgaa agtctgggcc agttcttcat tagatagatt caggccacct
    11221 ttgagcagag aatacgcttc agcaatgagc tgcatatctc cgtattcaat accattgtga
    11281 accatcttca cataatggcc tgcgccgtcg gcaccaatat aagtaacgca tggctcgccg
    11341 tcttctgcca cggcagcgat tttagtcagg ataggcgcaa ccagctcata agcctctttc
    11401 tgcccgccag gcataataga aggacctttc agcgcgccct cttcaccgcc ggaaacaccg
    11461 gtaccgatga agttaaagcc ctctgctgaa agctcacgat tacgacgaat ggtgtcctgg
    11521 aagaaggtat taccaccatc aatgatgatg tcacctttat cgaggtatgg tttcagggaa
    11581 tcaatcgcag catccgtgcc tgcacctgct ttcaccatta acaggatgcg acgaggcgtt
    11641 tccagagatt caacaaactc tttcaccgta tagtaaggag ccagtttctt gcctggattt
    11701 tcagcaatca cttcttccgt cttttcgcgg gagcggttga aaatagagac ggtataacca
    11761 cgactttcga tgttgagcgc aaggttgcgc cccatcactg ccataccgac tacgccgatc
    11821 tgttgctttg acattgttta ctcctgtctt ctatattatt aaccgtctat taaccgttat
    11881 attattaacc gtcgtgattt tcatcttcta atcaaggaat acatatggtt acagcatgct
    11941 tcttacttta tagcgataag tatgccattg ggtgtatcca taagaacttt gaattttgga
    12001 tacttttctg gattaaaatt agcatagctt cgtttagata taacaataaa cgaactatta
    12061 aaagatttca tcttctctat taaatcatcc tgtgggatta cctcacactc gggattacta
    12121 atagtccaat cactatatct tgctaatcca aagacgtcac caattgccat ccctttatag
    12181 aagtaaatac catcctccaa gcctaattgg ataagattat ctccatataa ctcactatat
    12241 tcacttgctt tcttcatcag ttcatagcta tagcgacttt ctaagcgtga attaaaattc
    12301 aagtattcag aataaaaata tttgaattca aaatacaacg atcgccccga aaaaaaataa
    12361 tacacacaag gataaaatat cgtcccaact tgtaatttgt aatgttgata tcttttatta
    12421 aacttgcaat tgagatcgca acctgcaata ctgacaatgg gtaaatgggt gataaatacc
    12481 tgtcaccttg cgtaacagaa aaccaaaata agacatatgt taaaacaatc gagaaaaata
    12541 cccaaagtat tttattattc ctaagtgtaa agacagacag tatagcataa aaaacaacat
    12601 cactgccaac tttattaatt gcttgttgca aatctaatgt ggcaggactt ataccatgtc
    12661 gagcttgttc tgctgtttga acttgtagat caatttcatt ccataggtag tgaccaaata
    12721 tatttcctcc agcaggacta aaaggatcac cagagatcca aaaactacgg acataccaat
    12781 aaatgccacc aagaattgtt gccaagctaa aaataagaaa tgtctgtatg tttctctttt
    12841 tcgctatcaa tataacaaat atgatggctc catatatggc accaaatagt tttacgccac
    12901 aagcaattcc aataaaaaaa ccacataaaa aaattaattc attttgtgtt ttattgtttt
    12961 ctgagcgaat atatataaga atactggctg cagcaaaaca aagatagcgt taaatcaata
    13021 tatgcataac caagatgatc tttaagtgga ttgatagtaa atagtaggat tgtagcaata
    13081 atgcctggat aataagtttt cgtaatttcc catgaacagc caacaagtcc acaggctatt
    13141 acaaaaaaac agatatttgc taaaaactga gctaacctta catctcctaa ctgtaaacct
    13201 aaagccatta aaagatctgc attttgcggg aacagaggaa aacgcaaata ttgttcaaca
    13261 acaattgcat ggtgctcaac taatgaacga gccaaaggta agtgatacat agtatcatcc
    13321 caatcaccgg gagctcgcag ggacgataac aatgtaacca caaaaatcaa aacaagaaat
    13381 aaaataacaa acagcgtact gaactcaata ttatttattg ccagccatct aaattgctga
    13441 cgtttaataa tgcttaaaac ccaacacacc gtaaaaatta aaaagatagc aatcttatta
    13501 aattgcccga ccatgcccag aaagtaaatt atcaaaatat ttaaagacat ccctatgcat
    13561 aggcaataaa aaataaagaa aatttgttaa gtcctgccac cccctcctta aaaaaagatg
    13621 acaatacaat attaccaatg acaaaaaatg tcattccaaa agcaaaacaa tacacaacat
    13681 acaaatacaa cggcaattca atcatagtaa aatactctat ttacattatc agtttatagt
    13741 ttttttcaga aagataaaat aatgccttca tttcacgtct agttgttgta tttgaatgca
    13801 aaataatgcc acatacaaat gccattattg acgataacat gatggaggca gttaaaaagg
    13861 cagtcggaaa tcttctcacc aaacctgtat caatataatc aacaatgatg ggcaatccta
    13921 aaattataga caaagcagca agtaaaacgc cgataatact aaaaaagtat agtggccgtt
    13981 caagcgagta aagtttaatt attgttttca gaatcttgaa cccatcactc caagttgata
    14041 gtttgctcac actcccttca gatcgctcgc catattttgt attaacctca ccatacttca
    14101 tacgtaattc taaagcatga attgtaagct cagtttcgat ttcaaatccc cttgatagac
    14161 atggaaaaga tttaacatag cgtctggaaa atacacgata ccccgacaac atatcagtaa
    14221 aattcccttt aaagatagtc tgaacagtac cagtcaacag tttatttcct cagcgatgcc
    14281 cttttctgta tgtattttga tctccattct cttgacgaca tcctacaacc atatccaatt
    14341 gattttttat gagatggtta atcatttctg gagcagaact tgcatcataa gtgtcatctc
    14401 catcaaccat tagataaata tccgcatcta catctgagaa catacgtctt acgacattcc
    14461 ccttgccttt cagaggaaca gagtgaactg ttgctccagc atcctctgcc acttgtgctg
    14521 tactgtcagt cgaattatta tcaaatacat atatagaagc agttggaata ttcgttttga
    14581 aatcatttat gacttttgct attgccttct gctcattata acaaggaata attacagcga
    14641 tattaaaaga gtgatacata agcccctcat ataaactatt tagctaatgt gcaagtaatt
    14701 gtgtttttaa caaatcgttt actaacgaaa aatgaaatat tttgatgcgc aatatcctaa
    14761 aaccaaacta aaaagagaaa aggtgaacaa tgtaaatatt ggagataaag ctaagagatc
    14821 aaataaaacg ccgaataaat aactcatcgc ccccataaaa aatataaata taaaatatct
    14881 agccttggtt gcagatactt taaatgagca tcttgcattt gcaaaaaaag aaaaggttgc
    14941 tgcacatata aaaccaacaa tgttacttcg gccttgacca aaagctaaat tattataaca
    15001 cgcataaaat accccccaat gtaaagcagt attgacaatt ccaattaaaa aataactaaa
    15061 aaaagaactg gtcaataact tccctatttt taacatcctt tattttgctc cagaagtgag
    15121 gttattacta atttggatat tttctataga aaatacccat aaaactacaa cgcctataat
    15181 aaaaattccc atgggtgtaa aaagaaatgg attagttata gaaacggtaa tactgaaaaa
    15241 aagtattaac gacatcatta atgatgtttt tgtactattt aaaaaatagc ctttcaaaaa
    15301 gataagcaac ataggaacta atgatataaa tacaccaata acgcctgttt tctgaagtat
    15361 ctcaagaggt actatttcaa tccttaatcg atctaatata aatgaaccaa atcctcttcc
    15421 aaacaagaac gtcgctaaat caacattttt ataataaaaa tataaatcat taaatctaac
    15481 agagtcagaa tcttcactgc gtgatccgac catgtaaagc ataattataa atagaacgct
    15541 gcccaatatt attataatag tatttaaata tagcttatgt aatttaaaac tggctaataa
    15601 cagtgataat gttgtaaaaa gtaatacacc tcttgattct gtcaataata tcgcaagcac
    15661 tattatgaga cattttaaat attttttgtc gacaaaactg ataatcgcac cgacaccaaa
    15721 aaagtagaag gatttggaaa aaaaagcccc atcgggacga aaaaagaact cgctagtcaa
    15781 tgataagtgt tcataaatta aattaaaatt taacaacccg aaattcaaca aaattatgta
    15841 tgaaatatat aacagtgatc catataatgc aaatgcatta actattttaa taaaaatctc
    15901 acatgaaaat acttttaaag tatatcttag tgtaaataga tatataagtg acagcataaa
    15961 aaggtatggt tgcacatcaa acagtatatc atttatttta ttaccagcta ataagccgac
    16021 aaagaagcca taaaaaggaa ataaaagcaa ccatataaaa agactgacac atttttgggt
    16081 gattgaaaaa ttgaatgtaa ctaaattaaa aattaaaagt ataaaactaa atacaaataa
    16141 taaatatcgc aaagggaata ttcccaatgg ctccagtaat cttccaccac caccaataat
    16201 cagttcaata cataaaaatg ttataaaaat tttatttata ttattcatat tcgtaaggtg
    16261 atgtttttta aaatatgttt aaaatcaaca ttaataaaat ataaatttta tcagcctttt
    16321 tccgccttaa cttccaaaaa cttaaagcca agatcaaatt gcctatataa cctttaccca
    16381 attgattatt cacaaagcta tcatcagcta aggcattagc tattttagtt acttcttttc
    16441 gataccactt gtttctcacc aaaccaagcc gtttgtattt tgctttaaaa ctaatgtttg
    16501 ctcccacttg gttgttttca tgctgtctgt acagcattgt tggttttcta tcaatatacc
    16561 acgaataatt tctcgtccgc gcaaatgaat ataaaaacca atcatgcaga cagatatctt
    16621 gtgcagcatt tttattattt atgatgaact tcttaaattc aataagcgtt tcttttttta
    16681 taacatatgt acaccccgga cctgctgctt caaaaaaata atcgaattga gtttgcgact
    16741 gagccttatc aactaaattt tttcgccctg atggataata tgcaattaca ttgctggaat
    16801 aacaattcgc attattaaat accaagcagt caattgcatt ttttatttta tctttatacc
    16861 aaatatcgtc ctgatcacta aaacagatat aatcatagtt ttcaagatcg acatctttaa
    16921 ttaatctata aaaattttta cctgcccccc caaacttatt gacgcttgga agtatattta
    16981 taattgaggg atatgataat tgaaattctt cacaaatatt taatgtatta tcagttgata
    17041 aatcatctga tataaaaatt gagatgtcta catctttttg ttcaataata ctttttaatt
    17101 gttcctcaat ccagcattcg ccattatacg tagccataag tacagctact cttttttgat
    17161 cagaactatg cattataacg accgccccca gtaattcctc ttattccatc cagcgtagcc
    17221 aaaatatgtt ttttcatata ttcaatttta tttttttgag ttgcgataat taaagtcgta
    17281 tgaattaaag aaaaaacaac ttccctaata gagagtaaaa gaggcacatg attttttcta
    17341 agcaaaagaa atgcatttct tactcgataa taacgacgta ctggcgagtg aattggtatg
    17401 ttaatcccac agatttttac tgacttatcc ccaatagcat gaaccatttc tatatcagga
    17461 atgatatgaa ctaatatgcc atagtttaag catctaagac accattctgt atcgacataa
    17521 tcaataaaaa gttcctcatc catcattcca acaatctcaa aaacttcttt tgaaaccatg
    17581 gttccggatg agattgttac tgaactttta aacggtgtct gtccttcagt gacatgaatt
    17641 ttctctcgaa gaccattttt tttgatatta caaataggat aataatgtga cttatctcta
    17701 tcaataaaaa ctgggccagc tatttttgca ttttcattat ccatatagca ggaaagtttt
    17761 ttgataaact cattgcgaat acggctatct tgatcaaaaa aaattatctt ctctgcccct
    17821 ttttcaagag aatatcgaat cccaatattt tgtgcttttg ctataccttc attcctacca
    17881 agacaaatta aatgaacttt ttcaatgtct gctaatttat ctttcaaggt gaaattagtc
    17941 ctattatcga ctactacaac gtaacaattt tgctccttta gttcagtgat cagtgcattc
    18001 agatttttaa gttcgggatt atatgtcaca atgacagcgt aaatattact attcattatc
    18061 caagtgactc agtaattggt taattttagt tttcatatac agaacacatt ctagtataaa
    18121 gccattgtaa taacagagca tctaaaatta cacgtatgct ccaggatatt gcggcaccca
    18181 gtaacccata tttttcgacc ataaaataca aaagggctaa ataaggaacc aattcagcac
    18241 aatgaatcaa agcagtaatt ttagattttc ctaatgcctg gattgcagag aatggtatca
    18301 ttgccagcgc attgaagaaa aaaccgatca ataacacatt taatatatta aaggaattaa
    18361 ttttataatt ctcaccaaac catattttca atacaagccc tgaatataac aagcctatga
    18421 ttatcaccgg caaacagatt agaaacatta aaagcaatga tttatttaca ttacgtttaa
    18481 attctttaaa gtctttgatg ttacttaacc ttggaaacac tgccctcccg attgctgcag
    18541 gtattattcc taattttaga ataacctccg ctggtgcaga ataaaatgca actttatctg
    18601 cccccataat atttgacact ataaatcgat caaaatacac catgaccgga cttatgatat
    18661 tacttactgt catccaaccg ccaaagaaaa agagtcgttt aaaggtttta taacaaaaat
    18721 gaacccccga aattttaata tcattcctga cataataggc agagactaaa atcgaaataa
    18781 ctcttgcaaa tattaaacca gcaaccgctg ccgacaatgt agcactataa aaaacaaata
    18841 tggccggaat tcccgcgatg caagagcttg atatagattt ttgaatattt acaatgccaa
    18901 atttttcatc cccctcaaga atggctgacc ataattgatt tagaataaat aatggaatgc
    18961 aaatagccaa cagtttaaat gctagttgta tatcactatg ttcaacacca gaaattttta
    19021 gataattaac aattccatca gagaaaatca ataataaaaa agcaccaaag catgaaaata
    19081 gcaccaaaaa agatgtactt gttgaaatta ccttggttct ttcatgatga ttatcacgat
    19141 gaattgcaat ttctctaata acagagcgag tcaggcctac atcaaaaatt ccagcatacc
    19201 caacaagtgc aattgcaatt gtataaatac cgaaaccttc aggccccaaa cctcgagcga
    19261 gaaatcctag cgaggggata gcaattaaag ttggtattgc atagccaaaa aggttccaga
    19321 cactattttt tattatgctc attattacat ttctttatca tataaaataa tcagcatcta
    19381 caaatttttg accattaata tctttatcag aaagcaatgg attttgaacc ggccattcta
    19441 tatttatact tttgtcgctc cataaaatcc cacgctcttg catttttgaa tagaaattat
    19501 ttgttttgta gacaaattct gcttcatcac taagaacgca aaacccatga gcgaatcctt
    19561 ccggtatcca taattgacgc ttattctccg ccgacaaatt tacaccaacc cacttgccaa
    19621 atgttggtga tgatttccga atatcaacag ctacgtcaaa aacttcaccg acaacacagc
    19681 gtaccagttt tccttgagca taaggttcca gctgatagtg cagtccgcgt aacacaccct
    19741 tagttgattt agaatggtta tcctgaacaa actcaacctt tcgtcctaca gcctcttcga
    19801 aaactttctg attgaagctt tccataaaaa agccccgctc atcaccaaat acttttggtt
    19861 caaaaattag cacatcagga atttcagttt taattacgtt cattttatta ataaccttta
    19921 atcattttca gcagatactg accataagca tttttcttca gcggctcggc taataccttc
    19981 acttgctcag catcaataaa gcctttacgg taagcaattt cttccgggca ggaaactttc
    20041 agcccctgac gctcttcaat tgttgcaatg aagttgcttg cttcaataag actttgatgt
    20101 gtccccgtgt ccagccatgc ataaccacgc cccatcatag cgacagacaa acgtccttgg
    20161 tccatataaa tacggttaat atcggtaatt tccagttcgc cacgtgcaga tggtttaagg
    20221 tttttcgcca tttctacaac gtcattgtca tagaaataaa gcccagtaac cgcatagtta
    20281 ctttttggtt ccagcggttt ttcttccaga ctaattgccg taccgttatc atcaaattcc
    20341 accacaccgt agcgctcagg atcatttacg tgataggcaa aaaccgttgc gccactctct
    20401 ctgttaacag cggtatccat taatttcggc aggtcgtgac catagaagat attatcacca
    20461 agtaccagcg cacaatcatc accacctata aattcttcac caatgataaa tgcttgcgcc
    20521 agaccatccg gactcggttg tactttatat tgcagattaa gcccccactg gcttccatca
    20581 ccaagcaatt gttgaaaacg tggtgtatcc tgcggcgtac tgataatcag aatatctcga
    20641 atacccgcca acataagcgt tgaaagcgga taataaatca tcggtttatc ataaatcggt
    20701 agcagttgtt tgctcacagc cattgtcaca ggataaagac gagtgccgga accaccagcc
    20761 agaataatac ctttacgcgt tttcatttca ccattccttt taattcatct cgctctagca
    20821 tcatgagcga gatgcaataa tttgttaaat tgccgtggtc gtaaataatt cgttgagcat
    20881 acgtttcacg cccacctgcc agtcaggcaa gacaagcgca aagttctgct gaaatttttc
    20941 ggtattgagg cgagaattat ggggacgacg tgctggtgtg ggataggccg ttgtcggcac
    21001 ggcattaagt ttgttaagag caaggttgat ccccgctctg cgcgcttctt caaataccag
    21061 agcggcataa tcgtgccagg ttgttgtacc accagcaacc agatggtaca aaccagcaac
    21121 ttctggttta tttgcggcca cgcgaatggc atgagcggtg caatcagcca gtaattcagc
    21181 ccccgttggt gcgccgaatt gatcgtttat tacagccaac tcttcgcgtt cttttgccag
    21241 gcgcaacatc gttttggcaa agttatttcc tttaccagcg tatacccagc tggtacggaa
    21301 aataagatgc tttgcgcaat gttcttgtaa cgctttttcc ccagctagct tggtttcacc
    21361 gtagacattt agcggtgctg ttgcatccgt ttccagccat ggcgtgtcac cgttgcctgg
    21421 gaaaacataa tcagtggagt agtgtataac ccaggctccg acttcattag ctgcttttgc
    21481 aatcgcttcg acgcatgtcg cgttaagtaa ttgcgcgaaa ttcggttctg attccgcttt
    21541 gtctactgca gtgtgagcag cagcattaac aataacatcg gggcgaattt ttttgacggt
    21601 ttcagccaca ccttcggggt tgctgaaatc tccacaataa tcagtggagt gaacatcgag
    21661 cgcaatcaag ttacccagag gagccagagc acgctgcagt tcccaaccca cctgccctgt
    21721 tttgccgaaa agcaggatat tcattactga cggccctcat agttttgttc aatccacgac
    21781 tgataagcac cgcttttcac attctcaacc caatttgtat tagccagata ccattccacc
    21841 gttttacgaa tcccgctctc aaacgtttct tgcggtttcc agcccaattc gcggctaatt
    21901 ttatcggcat caatggcgta acggcggtca tgccccgggc gatcggcaac ataagtaatt
    21961 tgctcacgat aagatttctc tttcggtaca atctcatcca acaaatcaca aatagtgagc
    22021 actacgtcga tgtttttctt ttcgttatgt ccaccaatgt tataagtttc acccgcttta
    22081 ccttcggtta cgacggtata taacgcacga gcatgatcct ctacatacaa ccagtcgcga
    22141 atttgattcc ctttaccata aataggtaat gccttacctt ccagagcatt cagaataacc
    22201 aatggaataa gtttttccgg gaagtgataa ggaccataat tgttcgagca attagtcaca
    22261 atggttggta aaccataagt ccgcttccag gcacgtacca aatgatcgct ggaagcttta
    22321 gacgcagaat acgggctact tggcgcgtaa gctgtcgttt ccgtaaatag cggtaacgct
    22381 tcgttattat ttgcttcatc cggatggggt aagtcaccat aaacttcatc agtagaaata
    22441 tgatgaaagc ggaaactttt tttcttttca tcattcagag cagaccagta attgcgcgcc
    22501 gcttctaaaa gaacataagt ccccacaata ttggtttcaa taaatgccgc agggcccgtt
    22561 attgaacggt caacgtggct ctctgccgcc aggtgcatca ccgcgtctgg ctggtgctgt
    22621 gcgaaaatac gcgacatggc tacagcatcg cagatatctg catgctcaaa agcataacgt
    22681 tcagaatcag aaacatcagc gagtgattcc aggttcccgg cgtatgttaa tttatcgaca
    22741 ttaacaacac tatcttgcgt attatttatt atgtgacgaa caacagcaga accaataaac
    22801 cctgcgccac cagtaacaag tatcttcact tttttattcc atatagctat agagcatgct
    22861 gtgaaataga ctgctccaga tttgattaat agaggtatta atacacgcta ccgcccctgg
    22921 cttaacagct accagtgcac tgcatgcatc tcaacgatgt ggcgagcgtt agccactcgc
    22981 gccaaatccg aaaaattcaa acgttaattg tcttaccaaa cagccgtaga aacaaggaaa
    23041 atcctggaaa aatttgaata atatgagcta tgtaacttct tgtttttata ggctttataa
    23101 aaacggcaac ggcaggaaaa ttcaatgagc aaaacttatg ccacgaatct tcactgccgt
    23161 tataattttc ttatcaaccg ttacatccgg tcagattttc attattcgct taacagcttc
    23221 tcaatacctt tacggaactt cgccccttct ttcaggttgc gtaggccata cttcacaaac
    23281 gcctgcatat agcccatttt tttgccgcag tcgtaactgt cgccggtcat cagcattgca
    23341 tcaacggatt gttttttcgc cagctcggca atagcatcag tcagctgaat acgtccccat
    23401 gcaccaggct gagtacgttc cagttccggc caaatatcgg cagaaagcac atagcgacct
    23461 acggccatga tgtctgagtc cagcgtctgc ggctgatccg gtttttcgat aaattcaaca
    23521 atgcggctga ctttaccctc acggtccagc ggctctttag tctggatgac ggagtattca
    23581 gagaggtcac ccggcatacg ttttgccagc acctggctgc ggcccgtttc gttgaaacgt
    23641 gcaatcatgg cagcaaggtt gtaacgtagc gggtcggcgc tggcatcgtc gatcacaacg
    23701 tctggcagta ccacgacaaa tgggttgtca ccaatggcag gtcgcgcaca caaaatggag
    23761 tggcctaaac ctaaaggttc gccctgacgc acgttcataa tgatcacgcc cggcggacag
    23821 atggactgta cttccgccag cagttgacgc ttcacgcgct gctcaaggag tgattctaac
    23881 tcataagagg tgtcgaagtg gttttcgacc gcgttcttgg acgcgtgagt taccaggagg
    23941 atttctttga tccctgcagc cacaatctcg tcaacaatgt actgaatcat tggcttgtcg
    24001 acgattggta gcatctcttt gggtatcgcc ttagtggcag gcaacatatg catcccgaga
    24061 cccgctacag gaataactgc ttttaaattc gtcattattt catccacctg taaaatggtt
    24121 gctgaattat agcttgttcg atttttttcg ccagcatcaa ttaccctgaa ttgattactg
    24181 aattacttgt gatgttacgc cgcttcgttg tggattgcag tagcattgtt cctaagtatg
    24241 gctccatttt tccaggaatg gtcgcaaatc tactccctcc gttccggcaa tctaaagtta
    24301 atcttttcca cattaacaat atggtgatta atcctgtcga tatcgacgga gctttgccct
    24361 ctttcattca ccgcatgaac atttgcaaga gacagcagtg tttctttttt cgccataaag
    24421 acgccgcgaa cgtctttgcg catgtcgaag ttcatgctca atgctggtcc aactgaggat
    24481 tcctgcatca cattgatatt tcgcataaaa agatgttgcg gcttgttgtg taactccagc
    24541 gaggcacgct tcatctcaat gttagttagc gacacaaagg agacggcatt cccggcggag
    24601 atttggatgc cgcgcaattt ataagcaaga tgggtattat ccagttgaat attattcact
    24661 cggaaatttt gcggtatcga gagatatttg cctttaatta ccccatagcc gattaacatc
    24721 ccggcgctat taatcatttc aatattatca atcacgaaat tgtcacaacc gtaaatagcg
    24781 actgtcgcgt tatcaatgcc cgctttctta ctgaaatccg gcgtgatatt gcgggcattg
    24841 atattacgaa taacaaaatg tttaccattt tcaacatgga tcaactgccg acaatccgat
    24901 cccgtgatat tcgccacgac aaagtttttc actgcctggt cttccgggta gttgttatca
    24961 taagtgcttc ccgcaaggcc tatgccgatg ccccagttga ttttgccgtt ggtacagttg
    25021 atacgctcga tgacatggtc agatatcaaa atatcactgt cgttaattgc cacgttccat
    25081 tcaatggcgt cgccctgtaa gtcgctgaac ttacagttgg taatgttggc accgataatc
    25141 tggttatgaa atccctggcg taagatggcg taattagcgt ggctgacagt gaggttatcg
    25201 atggtcaggt tgcgcatgac ccgtttgttt ttgccgccaa tataaatctg cattaccggg
    25261 ccaaagccgc tcatcgccag ccctttgatg gtgcagtcag aacaacgcac atccagggta
    25321 atgttatgca taccgccgcc ctctccccct gtcacctggc tgccgtcctg taagacaaat
    25381 cgccctctgc cgttgccgcg caggcttcca aggatgtgta acgttttgcc gggagggatg
    25441 aagatgccgg tgttgatatt gtcacaaacg aatccggcag gcacgacgac cgtttgccct
    25501 tcgctgaagg cttgtttaaa tgaggcgatc cagtcatgtg ggttgtagtc gttaatgtta
    25561 acgctttgtc gggcgggaag tgcgcgagcg aaaggggtat ggaggaaggc aagcgccgag
    25621 cttgccgtca ggaaggtgcg tcgggagagt gttttaaatg gcatacgttc tcctctataa
    25681 agcctgcagc aagctggcga gttctcgatt aatcacctgc tggttaaaat cgtgttcgac
    25741 tttttcgcgc gcacgtttga cgaccggagc cagttcgtcg gtatccagtt ggctaaacgc
    25801 cgccagacgt tgtgccagtg cgcgagcatc gttctcaggc agcagccagc cggatttatc
    25861 ggcctccacc agttcaggaa ttccgctatg cagagtagaa accaccggaa taccgaccgc
    25921 catcgcttcc attagcgcca ccggaatgcc ttccatatcg ccatccgcgc ccgttaccga
    25981 tggcaacagg aagacatccg cgtcgtcgag catctccttc acttcatggc tcggtttaaa
    26041 gcccggcatc tctaccacat cttccagttg atattgttcg atgagggtgc gcaggcgtcg
    26101 ttcccacagg ccaatgccga ggatgcgata gcgaaatgcc acgccctgct ctttcaactg
    26161 acggcaggct tcaatcgcca catgcaggcc ttttttctcg gttaagcgtg cgacagatat
    26221 gatttccagc ggcgttgcgg gcgctttcac cggacgcggg ctaaagcgcg tcatgtccac
    26281 gcccatacgc gatacggcga ttttttctct ggggcagccc attttttgca gccttccggc
    26341 ccacagatcg cttatcggta acatcaggtc gccacggcga aacagttgtt gatattcggg
    26401 agtgtagtgg ttgagcactt cccgactgga gatatcaata ccgtggaaga tggtggcaat
    26461 tttgccgcga atgataccca gttcgcgtag ttttgctgcg gttacccccg ctgggccaaa
    26521 atgagcgatg aacacatcgg cgcgaaacgg tgttgcgacc tgaccacaaa tggcagacaa
    26581 aatcaggttc cgcgactcgg caccatagcg tttgaggttg agcgcatgcc aggtattttt
    26641 acgatgaatg ccacgtaagg tctggctggc gcggtggcgc agtttcgcta ctttgcccgc
    26701 aggttcgtcc tgtaaccagc gggttctggc gacaaggttg tatttcgtcc atgccgcgtg
    26761 ggtgttctct gtatcgcctt tttgcaaagc gacaatctcc acttcaaagc ccatatcaat
    26821 aaacgcggta atttgattga ggacgaaggt ttccgacgac agcgggaatt tcaataagaa
    26881 gaagccgacc ttcatttcac ctccccgatg cgctcaagca cagactgcac catctgcatt
    26941 cccgtctgac gttcgcgact gacggcttcg ttaaatcgag cgttcagcac cggaagctgg
    27001 cctaaggtat ccgcaaccat cgcttgcagg ctgccgtcca gtaaatgacg aatatcaatc
    27061 gccatctctg gcagtcccag ctgctgcata atcccggcgg atttatgttc atagttgatg
    27121 gcaatcgccg gagtagcaaa gttcattgag ataatggcgg agtgcaggcg cgtaccgacg
    27181 gtgagttcac aggctccgag aattttgccc atttccagat cgttgagttc atccatcact
    27241 acgtggtaac gggcaggatc gctgatgtgc tggcgcagat tgagcgccac catgcggtcg
    27301 tctttgttat agctgtcgat acccgtacag gtggagagcg caatcacctg atagccttca
    27361 tcgagaatgc gattgaccac cccggcaaag gctttttcat acgcttgttg agtggtgccg
    27421 agacgtttgt cgaacggtgc cagttcgcgc agggtaattg ccactgtttt ctgttgtgcg
    27481 gcaacgtcca gccagtgttg aacggcatag ctggcggtga agtcttcggt gtggtgatcg
    27541 accagccacg cggtatcgac gccatgttcc acttttgcgg tggtgatatt tctgcgtttc
    27601 atcaaatcaa ggctgaccga ttcgcgcagg atcagcgcgt cgcagtgacc aaagacatag
    27661 ttcgccagtt ggttaaattg ctcatcctgg aaggggccga cgctgtgacc aatcataaac
    27721 agcggctttt tcgccataaa tgtgcaaagc gcatgttcaa actgcggcac gccgtagaga
    27781 tcgacaaaaa acgatccgcc gacctgaata atggcgtcgt aacctgacag caggcgcacg
    27841 aagtcggtga atccctgggc gatggcgata ttgcgcagct tgccagtgtc ggtgacgcgt
    27901 gagagcaata cctgatgctg gtagcggcga cggaggactt ttttaatgcg cccgacaacg
    27961 cccgccgcgc tattgtgttg tgtcatctgc aggaacagcg gatcgcccat taccgggcgg
    28021 ttgagcagcc aggaagaact gaccggatag cggctcatca cgtccacttc ggtatgtgga
    28081 ttgaggatgt tgatggcatc aagtaagccg cgcaagatgg cgctgtcgcc acgattgccg
    28141 caagtgtggt tgcccagaat aagtaatttc attttttcct cataaatttg atgccaggtg
    28201 aggctgtgtt tgttgtgttc gttgccgttt tttgcctgat gcgacgctgg cgcgtcttat
    28261 caggcctaca ggtcccgagc acagaaccgt aggacggata aggcgtttta cgccgcatcc
    28321 ggcaaccgtt gtcggaaccg aaaacagcaa ttcaccccgc ccgtaaaagc attttcattt
    28381 tttcgctgcg acaaaactga cgcttcactt ccaccaccag cggatggcgt gacagcacaa
    28441 tcatcaccac aaacgccagc acgcccgcgg ctatttgcac cgccagcagc atccccagcg
    28501 ccagttgccc tttcagcaca atgcccagcg cataactgac caccagcgtc ggcaacgaga
    28561 gataaaacgg cagccacaaa ctcaggatgt actggcgata actggaacca agaaccggtt
    28621 taatcatcac gaagtaactc agaatggtgt tgataatctg caccagcagg aaaccgagcg
    28681 tgacgccgat tgcgcccgcc atctgcccac caataacaat cgccggaata aacagaaatg
    28741 ttttgaatac gttgaattta aagctgatat cgacccgcgc tttcgccatc agcagcgaac
    28801 caatcgggtt acccacggag cgcagcagac ccacaataca cagcaattgc agcaccggaa
    28861 taatgctgtt ccacttttcg ccgaatacca gcggtacgaa gttattcgac accaccatca
    28921 gccccagcag cgccggaaag ttgataatcc ccactatcga cagcagcttg tagaagttaa
    28981 cacgcagctt ttcggtgtcg tcctgaattt tggcaaacgc cggaaacaac acgcgggtga
    29041 tgattgggtt cagcttcatc ggcggtacga cggccacgtt gtacgccagg ttgtatcccc
    29101 ctgccacgcc cgcgccaaga atacgcgcca gcaccagcgt tgaaaggttg gtgttgagat
    29161 agttgatgat gctgtccgcc gtcagccagg caccaaaacg taagttcggt gccacagacg
    29221 ccagcgagaa gtgcagaccg gggcaataaa ttttgcgtcc aaagtagcca aacagcagtg
    29281 ttctcaccgc actattgacc agataaccga ggatcgcggt catcgccagc ggccagaaat
    29341 gggcgctaac taccgtaaaa gtgaagcccg ccagcaccgc gctggtttcg atcatgccga
    29401 ttttgttgaa ctccagctcc ttctgcatca acgcgcggaa ctgttgcccg tgggggatca
    29461 ccacaaacgc cagcgacaat gttttaatca acggtgccaa gtccgggtta ttcagcacgt
    29521 cgccgatgac atcactcaac aaaaacaccg ccacacatac cacaagcccc agcccgacgt
    29581 tcagccagta cagcgtggtg agttcaaggt gactgatttc ttttcgctga ataatcgagt
    29641 tagcgatacc gaagtcagaa agcgtatctg ccagcgcgat aatcaccagc gacacagtca
    29701 gcaggccgaa ctggtggttg tcgataatcc gcgccagcac ggtcatctgc accagcccga
    29761 ggccgatgat gatcaccgtg gcaatagccg accacttcgc gccgctgatg gttttttcac
    29821 gtaagctcat atcaatatgc cgctttgtta acgaagccct tgaataccgt caggaaaacg
    29881 attttgatat cgaaccagac gctccattcg cggatgtact caaggtcgaa ctcgacgcgt
    29941 ttttccattt tctccagcgt gtcggtttcg ccgcgccagc cattaatctg cgcccagccg
    30001 gtaatgcccg gtttcacttt atggcgcagc atgtagcctt caatgagctg gcgatactgt
    30061 tcgttatgcg ctactgcgtg cggacgtgga ccgacaatcg acatcccccc ggtcagcaca
    30121 ttgataaact gcggcaattc gtccagcgag gtgcggcgca gaaagttccc cactttagtg
    30181 acgcgcggat cgttctgcgt cgcctgggtc accactttgt cgttctccat cactttcatg
    30241 gaacggaact tccacacttt gatcggcttg ccatccatac catagcgagt ctggcggaaa
    30301 ataaccggcc ctggtgaact gagtttcacc gccagcgcaa tacagcacag caccggggag
    30361 atcagcagca gaataagcgt cgccagcaca atgtcttccg cacgttttag caggcggtta
    30421 acccccgaaa gcggcgtgtc atacagcggc accaccggaa cgccgttcat ctcttcgaga
    30481 cgtgaatgga gaatgttgaa ggtaaagacg tcggggatca gcagcaccga acaggtggta
    30541 tccgccagtt gatggaccag ttttttcact cgtgcgccgt cgcacatttg catcgcgata
    30601 tagacgttat gaatcttgcc cgcttttgca tcctcgacca gctgttgcag gttacccgcc
    30661 cagtcgttag aaacgccgcc cggtttcggg tcgtggtaaa cgcccaccac ttcaaaccct
    30721 aaccacggct gattacggaa actctccatc agcatttgtc cggcggctaa atcccccact
    30781 acggcgacca tccgcttgtt gtagccatga ttacgcagcc agcccgcccc aatgcgaata
    30841 catgaacggc aaaccaccag tccgatgctg gtcagcccat accacgccag ccagattttc
    30901 agttgcgtgt cgaaatcatt gttgaacgcc accagtccgg cgctgaaaat cacgcttaag
    30961 gtccagtttt gtagcaacag ggcaaattct gtcgctgccc gaacaccgcg ccatgagcga
    31021 taaaaatcgg tgatgccgcc cagcatctgg aacaccacca gcgtaatcag cgccaccaac
    31081 aggtgcatgt agaggaatga cagtccgctg acttcgcaaa ccagccatag cccggcaaac
    31141 atgatggtga tatctgaaaa gcgttgcacc atagagatta acgatgcatt ggttttcgct
    31201 cgctcgcgct tttttagatt tgtcatcgtt gttcctgtta ttagcccctt acccgcagtg
    31261 gggtaaggga agatccgaca ttactcgttc agcaacgtca gcagagttcg cgttcgcgct
    31321 tccatcagcg gcacatcacc gcgcgattcc acattcaggc gcaccaccgg ttcggtattg
    31381 gaggtgcgca ggttaaagca ccagtcggca aaggtcatgc tgatgccatc gctgcgatcc
    31441 accgccagcg cctcacggct aaaatgctgt tccacgcggt taatcgcctc aacgggttgc
    31501 gccagtttgc tgttgatctc accgcttgcc ggaaacgccg ccatccggtc gcgcaccagt
    31561 tcgcccagcg tttttccttt caggcacacc agttcggcga ccagcagcca cgggatcatg
    31621 ccgctgtcgc agtaagcgaa atcacggaag taatggtggg cgctcatttc gccaccgtag
    31681 atggcgtctt ccttgcgcat ccgttcttta ataaaggcgt gtccggtttt cgacattacc
    31741 ggcgtgccgc ctgcagcagt caccacatca atagtgttcc aagagagacg cggatcatgg
    31801 atgatcttcg cgccgggatt tttttcgagg aacgcttctg ccagcaggcc gacaatgtag
    31861 tagccctcga taaactgccc tttttcgtca aacagaaaac agcggtcaaa atcgccatca
    31921 aaggcaatgc ccatatccgc gccgtgtttg atgaccgcat tgcgggtgtc gtcgcgacat
    31981 tccggcagca gcgggttagg aataccgttg gggaaattgc cgtccggtgt gttgtgtact
    32041 ttgattaatt ccacgggcgc gccgagggct ttaaagcggg cttcaatggc gtccaccacc
    32101 ggacccgctg cgccattccc ggagttgatc accagcttga gcggcgtgag atttttgaca
    32161 ttgatataac cgaacaggtg atcaacgtaa gcgtcacgca ggttgatttg ctgatagcga
    32221 ccgcgtttgg tttcatcgac gggaggaaag tcgttggctt cagccagacg ctggacgtcg
    32281 cgcagtccgg tatcaccgct gatcgtgcga gccccctcgc gcaccagctt catgccgttg
    32341 taatccatcg gattatggct ggcagtaact tcaatgccgc catccacgcc gagatggaac
    32401 gtggcgaaat agatctcttc ggtgccggac ataccgatat ccagcacatc gacgcccgca
    32461 tcctgtaaac cttttgccag cgccagtttt aaggtttcgc tggtgagacg gacatcaccg
    32521 cctaacacaa tggttttcgg tttgagaaat tcgccatagg cgcgaccaat gcgccaggcg
    32581 atatcttcat tcagttcttc gcctaatttc ccgcgaatat cataggcttt aaagcaggtt
    32641 aattttttca tgtcgttacc ctttttcagg caatagttgg ccctgaccga aacggccaat
    32701 tttatttttg tcgttattta caccgcggtt tcgcattcat tgcctgatgc gacgctgccg
    32761 cgtcttatca ggcctacaaa accgaaccgt aggtcggata aggcgttcac gccgcatccg
    32821 acaaacagcg cctgatgcga cgtttacacc cgtccgtagc gatccgcgaa gcgcaccaca
    32881 tcatcctctt cgagataaga gccggagcgc acttcaatta aatcgagcgg aattttcccc
    32941 gggttttcca ggcagtgcgt cgcccccagc ggaatataaa tggactcgtt ttcaccaagc
    33001 agtttgatat caccatcaat ggtgactttt gccgttcccg cgaccactac ccagtgttcc
    33061 gcgcggtgat ggtgcatctg taccgacaaa ccctcgcccg gtttcacggt gatgcgtttc
    33121 acctggtagc ggtcgcccgc gtcgatagag tcatatttgc cccacggacg atacacttcg
    33181 cgatgcaccc gatgctcatg gcgaccatcg gctttgatct gctcgaccac ttttttcaca
    33241 tcctgtaccg cgttacggtc ggcaatcagc accgcatctt tggtctgcac cactaccaga
    33301 tctttcaccc cgacggtggt gaccaggcct gattcggcat acacatagct gttttcagtt
    33361 ttgtgattaa tcacatcgcc gtggcaaacg ttgccctcgg cggtgtgggc gctgatctcc
    33421 cataatgaag accaggaacc aacatcgctc cagcccgcat ccatcggcac cacaacagca
    33481 tctgccgtag gttccatgac cgcgtaatcc accgactctt ccgggcaggc gagaaacgct
    33541 tcttcatcca cacgaataaa atcgagatcc ggatcgacgg cgctcatcgc tttttcgcag
    33601 gcatcgagga tatccgggcg atattttttc agttcttcga gatagcgtcc ggcgcggaac
    33661 aggaacatgc cgctgttcca gtaatattcg ccgcttgcta cataggcctg ggcggtttcc
    33721 agattcggtt tttcgacaaa ctgcgccact tcaaaggcca ccgtatcctg ctcacccaca
    33781 gacacttcac cgcgacgaat atagccataa ccggtttctg gtagatccgg cacaatgcca
    33841 aaggtcacca gcttgcccgc ttcggcataa ggcatggcat tacgcacggc ggcacggaac
    33901 gcgtcttcat cggcaatcac atgatccgcc gccaatacca gcattaacgg gtcgctctcc
    33961 gggctatgac gttttgccgc cagcgccgcc agtgcaatgg caggtgcagt gttacgccct
    34021 gccggttcga gaataatgtt ctcggtgagt ttgttcagtt gacgcagctg ttccgcgaca
    34081 ataaagcggt gctgctcatt gcaaatcacc accgggcttt cgcactccac gccgttcagg
    34141 cggcagatgg tggtttgcag catggtgaga tcgcctttca ggcataaaaa ctgcttgggg
    34201 taaagtacgc gggaaagcgg ccataaacgg ctaccggagc cacctgccat cacaactgga
    34261 tagagtttcg actgcgccat aattatcccc gaatatcatt tataaattga cgtaacacgt
    34321 tctctttatc gagcgtgcgt tcggcatatt cacgtgccac cgtgttatgt ttgggcagca
    34381 ggagcgcctg acgaatcccc gccaccagcg cctcgaccga ttccggttct acgcaaaccg
    34441 caatacccgg aaaggtttcg caaagctgcc ccagttctgt gtgggcctct gcggtaatca
    34501 ccgcgttacc gcctaccgcc agaatattgg tcagtttcga cggcaatacg gcatctgccg
    34561 cgccgcgttt ttgcaccacc agatggcaat cgcccatctt cagcagtgca ggtaaagcgt
    34621 catacgattg cagcgggaaa aattgcatgt tgcgcagtcc acgctgctgc gccatttttt
    34681 ccagccgcgc tttgccgccg ctctgcccga caatggcaaa aatcagcggt tcatcgcgca
    34741 ggagatcggc agcttcaata acgttttcca gcccctgctt ttcaccaata ttgccggagt
    34801 aaagaatgat ttttttgtta tccggcaggc caagctggtt acgaagggca tcaacatcgg
    34861 cgtctgcaac atgctgaaaa cgggcaattt ccgaccagtt ggggaagaag atgacgttat
    34921 ccgccgccac gcctttttcg atggctttat tcatcatcga acgcgaaatc gtggaaacgt
    34981 tatcgacgtt atgcagtccg ctacgttcga acgccgttgc cagctgtgcc actttgccgc
    35041 ctttgccttt tccggcaagg cccagcccca gcatggcgtc cacttcgtaa tcctgaatat
    35101 gcagcacggt acgcgcacca gagagcttcg ccagcaggcg cattcccggc gtgcaaaaca
    35161 gcgttggcac tacgccaata atgcgatccg gcttccagcg acgttgcgcc atcagtggaa
    35221 agaaactgct gacggcaaaa ctgccgagat gcaacaagcg tttcagggtg ctcggctgtt
    35281 ttggcacgta cagcgggcag cgccacaccg tggcagcccc ttcttctcgt ttgtagcgcc
    35341 aggcggaata gttctcaccc acctgccatt gcgggtagta aggcggtgcg gtaatgaccc
    35401 gcacctcatg accttgtgcc gccaaccatt ccaccatctc gccggtgtat ttgccgatgc
    35461 cggttaactc cggcgagtag ttaatgccgt agaccagtat tttcataatc cgggtactcc
    35521 ggcacgcttc tcagcgagaa aataggctcg gctgttagcg tgaacatcgt tactggcgag
    35581 cagcgcgtcc ggcgtcagcc agcggtaatc gtcatgctgc tcatccggca gtaacagctc
    35641 ttcttccgct actctgaagc gaaaaccgag caccacatag tgagtggtga aatccgtgcc
    35701 ggagaagtta tcgtcataaa aatgctgcca gacaccgtaa aactggcctg ctgttatcgg
    35761 cagacgcagc cccagttccg ccatcgtcag ccgctcaaat gcggcttcca gcgtttcgtc
    35821 tttctgcacg cgccctcccg gcacaaacca gtaaccctgc gccgggcggt tggttctttt
    35881 gccaagcaga aactcgccgc gactgttctc gacaataaag tcgagagaga caagcggtgt
    35941 ggagcgcact acagtggcaa agtcttcctg acgtaaaaac atcattcccc ccgaaagcgg
    36001 tcttgattct caaggaacca ctggtaagtg ctggcaagcc ccgcttccag tgagatttcg
    36061 tgataccagc caagctgatg caggcgcgtc acatccagca gtttgcgcgg cgtaccatcc
    36121 ggtttgctgg catcaaaaac cacccgacct ttgtaaccca ccactttggc gatggtttgc
    36181 gccagctcgc ggatagtgca gtcaacgccc gtgccgacgt taatgtgcga cagcatcggt
    36241 tgggtattct ccagccagac ttcatgcgcc agctccatga catgaatgct cgccgccgcc
    36301 atatcatcga cgtgcaggaa ttcacgcatc ggtgtaccgc tgccccatac caccacgtcc
    36361 gtgccgtcgc ctcgtggaag cgacgcagca atgctggtat cacatgggag ttactcgggt
    36421 ggaagttgtc atgcggcccg tacaggttgg tcggcatgac agtgcggtaa tcgcgtccat
    36481 actggcggtt gtaagattcg cacagtttga tcccggcgat tttggcaatg gcgtaaggct
    36541 cgttggtcgg ctccagcgtg ccctgcaata actcgctttc tgccatcggc tgttttgcca
    36601 gtttcgggta gatgcaggac gatccgagaa acatcagttt gttcacgtcg ttctgatgcg
    36661 cggcgtgaat gatgttgctc tcaatcatca tgttctggta gatgaaatcc gccggatagg
    36721 tgttgttggc aacaatgccg cccactttcg ccgccgccag atagacctgg tcaatgcgtt
    36781 cgctggcaaa gaaatcatgc acggcgcggc tgtccagcag gttcagttca tcgcgggtac
    36841 gtaataccag ctccacatca ccgcgctgtt cgagctgccg cttgatggca gaaccgacca
    36901 tcccacaatg gccagcgata aaaatgcgtt gtttactcat gcttatgact ccagcgcgat
    36961 cgccacgtcg tagccgtgag atttcagcag agagtgtttt ttcgccgctt cgaggtcatt
    37021 agccaccatt tcagacacca tctctctgag ggtgatttcc ggtttccagc ccagtttttc
    37081 gtgcgctttg gtcgggtcgc cgagcagcgt ttcaacttct gccggacgga agtaacgcgg
    37141 atcaacggcg ataatcacat cacccggttt aacgcccggc gcgtcatgcc cggtgacgga
    37201 aaccacaatg cccttctctt caacacccgt gccttcaaag cgcagtttga tgcccagctg
    37261 tgctgccgcc atttccacaa actgacgtac ggagtactga acgccggtcg cgataacgaa
    37321 atcttccggc tgttcctgtt gcagcatcat ccactgcatt tttacgtagt ctttggcgtg
    37381 gccccagtca cgcagggaat ccatattgcc gaggtacagg cacgactcca gcccctgggc
    37441 gatgttggcg attgcgcggg tgattttgcg ggtaacgaag gtttcgccgc ggcgcgggga
    37501 ttcatggttg aagagaattc cgttacaagc gtacatgccg taggattcgc ggtagttaac
    37561 ggtgatccag taggcgtaca gtttggcgac cgcatacgga gatcgcgggt agaacggcgt
    37621 ggtttctttc tgcggaattt cctgcaccag accatacagt tcagaggtgg aagcctgata
    37681 gaaacgagtt ttcttttcca gaccgaggaa gcggatcgcc tccagcaggc gcagcgtacc
    37741 catcgcgtcg acgtcagcgg tatattctgg tgactcaaaa gagaccgcaa cgtggctcat
    37801 tgcgcctagg ttgtacactt catccggctg tacttcacgc aaaatgcgcg tcaggttaga
    37861 ggtatcactc aggtcgccat aatgcagatg gaatttcggg ttgcaggtgt gcggatcctg
    37921 ataaatgtga tccacgcgct cggtgttgaa tgacgatgcg cgacgcttaa taccatgcac
    37981 ctcgtaacct ttttccagca gaaactctgc caggtaagaa ccgtcttgtc cggttacacc
    38041 ggtgatgaga gcgacttttg acatgtctta ttcctctgta tttttgaatt tattcagttt
    38101 caacgcgttc gcgtatcacc actgcgggat tcccccggca aaccacattt gccggaagcg
    38161 atttaaaaac actgcttcgt gcacccacga cggtgccgtc gccgattgtg acgcctgggg
    38221 caacaaagac atcggttgcc agccagcatt tctcgccaat cacaataggc gtggcgttaa
    38281 tggtgaaatg ttgacttgca tggtcgtggc taccggtgca taaataactt ttttgcgata
    38341 tcaccgaatg tgcgccaatg gttatttcac cgagggtata taaattgacg tcatcgccaa
    38401 cccacgcgta atcacctaag gttaatttcc acggataggt aatttttact gacggacgaa
    38461 taactacgtt ttttcctatt tttgctccga ataaacgtaa taaaaaagcc cgccagcgat
    38521 acaatacttg tggcgaccag gcaaatattg ttgcctgtac tgcccaccat aattgcactt
    38581 taatagcgtt gccgccccgg aaccctttc
//
LOCUS       DAJTQY010000028        37629 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.028, whole genome shotgun sequence.
ACCESSION   DAJTQY010000028 DAJTQY010000000
VERSION     DAJTQY010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37629
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.028"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(<1..176)
                     /locus_tag="OO349_001528"
     CDS             complement(<1..176)
                     /locus_tag="OO349_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HCR5866653.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVNP
                     LKLVNCDELNFQDR"
     gene            complement(260..1144)
                     /gene="yhdJ"
                     /locus_tag="OO349_001529"
     CDS             complement(260..1144)
                     /gene="yhdJ"
                     /locus_tag="OO349_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCR5866654.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDAFAELKKLPTESVDLLFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPKALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(1230..1526)
                     /gene="fis"
                     /locus_tag="OO349_001530"
     CDS             complement(1230..1526)
                     /gene="fis"
                     /locus_tag="OO349_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HCR5866655.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(1552..2517)
                     /gene="dusB"
                     /locus_tag="OO349_001531"
     CDS             complement(1552..2517)
                     /gene="dusB"
                     /locus_tag="OO349_001531"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HCR5866656.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSAPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(2846..3727)
                     /gene="prmA"
                     /locus_tag="OO349_001532"
     CDS             complement(2846..3727)
                     /gene="prmA"
                     /locus_tag="OO349_001532"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HCR5866657.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(3739..5190)
                     /gene="panF"
                     /locus_tag="OO349_001533"
     CDS             complement(3739..5190)
                     /gene="panF"
                     /locus_tag="OO349_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HCR5866658.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIIVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(5180..5422)
                     /locus_tag="OO349_001534"
     CDS             complement(5180..5422)
                     /locus_tag="OO349_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HCR5866659.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVDAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(5531..6880)
                     /gene="accC"
                     /locus_tag="OO349_001535"
     CDS             complement(5531..6880)
                     /gene="accC"
                     /locus_tag="OO349_001535"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HCR5866660.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSTDRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGKRCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(6891..7361)
                     /gene="accB"
                     /locus_tag="OO349_001536"
                     /gene_synonym="fabE"
     CDS             complement(6891..7361)
                     /gene="accB"
                     /locus_tag="OO349_001536"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HCR5866661.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(8339..9313)
                     /locus_tag="OO349_001537"
     CDS             complement(8339..9313)
                     /locus_tag="OO349_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR5866662.1"
                     /translation="MQALLLEQQDGKTLASVQTLDERRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASGILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            9465..11405
                     /gene="csrD"
                     /locus_tag="OO349_001538"
     CDS             9465..11405
                     /gene="csrD"
                     /locus_tag="OO349_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HCR5866663.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSALRPQITELMVSADIVRVDLLHGDKQVYTLARNGSYRPVGTSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIASQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTHVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            11710..12753
                     /gene="mreB"
                     /locus_tag="OO349_001539"
     CDS             11710..12753
                     /gene="mreB"
                     /locus_tag="OO349_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HCR5866664.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            12819..13922
                     /gene="mreC"
                     /locus_tag="OO349_001540"
     CDS             12819..13922
                     /gene="mreC"
                     /locus_tag="OO349_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HCR5866665.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGISQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPATGNAATA
                     PVAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            13922..14410
                     /gene="mreD"
                     /locus_tag="OO349_001541"
     CDS             13922..14410
                     /gene="mreD"
                     /locus_tag="OO349_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HCR5866666.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSMLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            14419..15012
                     /gene="yhdE"
                     /locus_tag="OO349_001542"
     CDS             14419..15012
                     /gene="yhdE"
                     /locus_tag="OO349_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HCR5866667.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADIIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            15002..16471
                     /gene="rng"
                     /locus_tag="OO349_001543"
     CDS             15002..16471
                     /gene="rng"
                     /locus_tag="OO349_001543"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HCR5866668.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            16539..20339
                     /gene="yhdP"
                     /locus_tag="OO349_001544"
     CDS             16539..20339
                     /gene="yhdP"
                     /locus_tag="OO349_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HCR5866669.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLNAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAAGKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSILKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            20495..21940
                     /gene="tldD"
                     /locus_tag="OO349_001545"
     CDS             20495..21940
                     /gene="tldD"
                     /locus_tag="OO349_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HCR5866670.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(22068..22997)
                     /gene="aaeR"
                     /locus_tag="OO349_001546"
     CDS             complement(22068..22997)
                     /gene="aaeR"
                     /locus_tag="OO349_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HCR5866671.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSANLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            23180..23383
                     /locus_tag="OO349_001547"
     CDS             23180..23383
                     /locus_tag="OO349_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HCR5866672.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            23391..24323
                     /gene="aaeA"
                     /locus_tag="OO349_001548"
     CDS             23391..24323
                     /gene="aaeA"
                     /locus_tag="OO349_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HCR5866673.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSDLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            24329..26296
                     /gene="aaeB"
                     /locus_tag="OO349_001549"
     CDS             24329..26296
                     /gene="aaeB"
                     /locus_tag="OO349_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HCR5866674.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            26388..26660
                     /locus_tag="OO349_001550"
     CDS             26388..26660
                     /locus_tag="OO349_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HCR5866675.1"
                     /translation="MNIYAFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(26716..26979)
                     /gene="yhcN"
                     /locus_tag="OO349_001551"
     CDS             complement(26716..26979)
                     /gene="yhcN"
                     /locus_tag="OO349_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HCR5866676.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDIREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(27344..27814)
                     /gene="argR"
                     /locus_tag="OO349_001552"
     CDS             complement(27344..27814)
                     /gene="argR"
                     /locus_tag="OO349_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HCR5866677.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            28249..29187
                     /gene="mdh"
                     /locus_tag="OO349_001553"
     CDS             28249..29187
                     /gene="mdh"
                     /locus_tag="OO349_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCR5866678.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKNCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGKEFVNK"
     gene            complement(29249..30316)
                     /gene="degS"
                     /locus_tag="OO349_001554"
     CDS             complement(29249..30316)
                     /gene="degS"
                     /locus_tag="OO349_001554"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HCR5866679.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(30406..31753)
                     /gene="degQ"
                     /locus_tag="OO349_001555"
                     /pseudo
     CDS             complement(30406..31753)
                     /gene="degQ"
                     /locus_tag="OO349_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
     gene            complement(31907..32305)
                     /locus_tag="OO349_001556"
     CDS             complement(31907..32305)
                     /locus_tag="OO349_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HCR5866680.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQVPRDYSEGASGLLRTGAKRD"
     gene            32499..33626
                     /gene="zapE"
                     /locus_tag="OO349_001557"
     CDS             32499..33626
                     /gene="zapE"
                     /locus_tag="OO349_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HCR5866681.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFTRGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGVKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            33846..34274
                     /gene="rplM"
                     /locus_tag="OO349_001558"
     CDS             33846..34274
                     /gene="rplM"
                     /locus_tag="OO349_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HCR5866682.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            34290..34682
                     /gene="rpsI"
                     /locus_tag="OO349_001559"
     CDS             34290..34682
                     /gene="rpsI"
                     /locus_tag="OO349_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HCR5866683.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            35077..35715
                     /gene="sspA"
                     /locus_tag="OO349_001560"
     CDS             35077..35715
                     /gene="sspA"
                     /locus_tag="OO349_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HCR5866684.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            35721..36218
                     /gene="sspB"
                     /locus_tag="OO349_001561"
     CDS             35721..36218
                     /gene="sspB"
                     /locus_tag="OO349_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HCR5866685.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(36261..37628)
                     /gene="dcuC"
                     /locus_tag="OO349_001562"
     CDS             complement(36261..37628)
                     /gene="dcuC"
                     /locus_tag="OO349_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HCR5866686.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGVFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASCAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKTLDNVPPLYYAILPVMPLILMLGSLFLAHVGLMKSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFVNVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIAT
                     ITLFY"
BASE COUNT         9114 a         9417 c         9898 g         9200 t
ORIGIN      
        1 attctgtcct gaaaattcag ttcgtcacag ttaaccagtt ttagtggatt aactgagttc
       61 atattacggc atttgtctgt ttctgttgtc agcagttcaa tccactgcat cagtaaagta
      121 tcaaacagaa aaacgaagac agcgaaaacg acgagccacc aatacttgcg aatcattacc
      181 tgagcctgtg catgtttaac caagtctggc aggaaatata cacgaaatga ataatctgaa
      241 aactaggctt acacttcctt tactttgcaa tgagatcggg gtcaacttcg cttaaccggc
      301 ttcgttttga caggttgccg gtctttcttt ttttcacttt cgccagttct tccgcggagt
      361 aatgcgacgc gacatccagc cgtcgaagcc ccattttgat gtactcgctg ttgatctcaa
      421 taccaatgaa ttttcgtccg ctggcgatgg ctacggcacc ggtagtaaag ctaccggcaa
      481 acgggtcgag aacgatatcg cctgggttgg aagaggcgag aataatgcgt ttcagtaagg
      541 ctttcggttt ttgcgtcggg tggttctcat attcatccat caaataacgc acgcgcggaa
      601 aatcccagac gttacccggt actttttgat gattgtatgg ctgtggagga tttttgcgat
      661 aatcgatcaa cgcgcgctgc gatccggttt tggcttctac cagaatagca tcaccgttga
      721 atgtgtagtt ctttgcgtct ttcaccatca tcaggatggg ttcgtacatg gagccgtagt
      781 gttttttcgc ctgtactcca gaactgtcat atgaccagac gatgcgactt ttgatggtaa
      841 aaagcttgcg gcactggaga tcgataaagg gcatgttttc cgtactgttc ataatgtaca
      901 tgctgccctg ctttttcaga acgcggtggc actctgcaat cacttcaaac agccagtcga
      961 taaacagatc ttctttccag gcttcgatca gaccatcaaa atttttaccg atgttatacg
     1021 gtgggtcggc aaagagcaga tcgacgcttt cagtaggtag ctttttaagt tcggcaaagg
     1081 catcaccgtg aataatggtc ttagcttcat taccaaaccg ggtcggttca catcctgttc
     1141 tcatggtcac tccctttgtg acacctataa aaaaggcgct tccccatacc gagtagcgcc
     1201 tttttaatca agcatttagc taacctgaat tagttcatgc cgtatttttt caatttttta
     1261 cgcagcgtac cacggttgat gcccatcatc agcgcagcac gggtctggtt accacgggtg
     1321 tattgcatca ccatgtccaa caggggctgt tctacttcag ccagtaccag ctcatagagg
     1381 tcattcacat cctgaccatt cagttgagca aaatagttct tcagtgcctg tttaaccgag
     1441 tcacgcaggg gtttttgggt tacctgatcc tgagagttaa cggtagaaac ggtcagtacg
     1501 tcagaattta cgcgttgttc gaacatagtt ctgtcagctc tttatttctg tttacgcaaa
     1561 attttcgaag tatgcctcca acgcctccag ctgttcgctg gcatcctcaa tggcgttgaa
     1621 tgtgcgccga aactggtcat ttggagcgtg ttcctggaga taccaggaaa cgtgtttacg
     1681 tgcaattcgg tacccttttg ccggaccata aaagtcatgc agttcccgaa cgtgcgcgca
     1741 aagcaagcgc ttaacctctg ccaaaggcag tgggggcagc aactccccag tgtccagata
     1801 atgctggatt tcccgaaaga tccagggtct tccctgagct gcgcggccta tcatcagggc
     1861 atccgcccct gtatagtcga gcacagctct ggctttaagc gggtcagtaa tgtcgccatt
     1921 cgcgataacc ggaatggaaa ctttctgctt aactgcccga atactgtcgt actcagcttc
     1981 tccattgaac aaacaggcgc gtgtacggcc atgaatggtc agagcctgaa tgccacagtc
     2041 ttcagccagt tgggcaatct cttcgcagtt acggtgttcc ggtgcccagc cggtgcgaat
     2101 cttcagggta acaggaacgt ccactgcatt gacgacctcg gtaaggatcg atttaacgac
     2161 atccgggtac tgcaagaggg ctgaacctgc gagcttgcga ttcacttttt tagccgggca
     2221 acccatattg atatcaataa tctgggcacc gctttccacg ttaatacgtg ctgcatctgc
     2281 catttctttc ggagcgctac cagcaatttg cacggtgcga ataccgggtt catcaatgtg
     2341 caccatccgt aaacgagatt tgtcgctttc ccaaacctgt gggttagaag acatcatctc
     2401 ggatactgtc aatccggctc ccatctcgta gcacaacgtc cgaaaaggtc tgtctgtaat
     2461 gccagccatg ggcgctgcga tcaggcgatt tctgagctga tattgtccga tgcgcatgag
     2521 ttaagaaatg accatactgt gactgcaagg cggcgtatat tacgcatttt ttgcacgaga
     2581 tgaaaggcca aactttgacc aatcctctga gatggatcaa agaattgcat ttaaaatgag
     2641 cgtggtgcga taattactca taaaaatcat catattagaa aatagtgact aaaatttaca
     2701 ctcaaagaaa tttgagtaag ttctcaattt ttctttatga atgaaaattt tggcacgcaa
     2761 atttgcgtaa ataatcggca aatttacgtg ccttttgtga gcttgctcgc acttcgcccc
     2821 gcgtcaccct acggcgatgc gaaggtcaat tcttacgacc ggtaatacgg caccactctt
     2881 ctttttccac gaccgggtcc agtgcgaagc tatcggcata agcttcacaa acgctctctg
     2941 cctggcttgc cagaataccg gaaaggccca gcaaaccgcc tgaaaccggc aggacgctga
     3001 ttaacggtgc cagttcacgt aatgggcctg caaggatgtt agcgaccacc acgtcggctt
     3061 tcatttcttc tggctgatct ttcggtaagt agagttccag acggtcagaa acgccattac
     3121 gttcggcgtt atcgcggctg gcctgaatcg cctgcggatc gatatcaata ccaatggctt
     3181 ttgctgcacc cagtttcagc gccgcgatcg ccagaatgcc ggaaccacag ccaaagtcga
     3241 tgactgtttt accggttaaa tcgaggctgt cgagccattg caggcacaga gaggtggttg
     3301 gatgggtacc cgtaccaaac gccagccctg gatctaacat cacgttgacg gcgttttcgt
     3361 ccggcacatc acgccagcta gggcagatcc acagtcgttc accaaagcgc atcgggtgga
     3421 aattatccat ccattcgcgc tcccagtctt tatcttctag ttgttcgatt ttatgcgcga
     3481 agcctgcgcc gagcagcgga tggttttcca gaatcgccac cacgtcgttc atatcggttt
     3541 cagcgtcgaa cagaccaatc acatcggtgt cgccccacag gcgcgtttcg cccggcagcg
     3601 gctcaaatac tggcgtatcg tgggtatcct gaaaagtgat agaaacggca cccgcttcca
     3661 tcagcgcatc gctaagatct tccgcgttcg cgccggtggt gttcagtttc agttggatcc
     3721 aaggcatggc aaaactcttt atttatcagt agtcaaaacg gtagcttgcg ggacggatgt
     3781 accgaaacgg tttccgacca ggaaagccag caaacttagt agtaacgagg gcacgatagg
     3841 gtggaagccc aggtactgaa tattcagcgt cgcgagtacg gcatacagca cgccgccaac
     3901 gatcatcgca cttagcgcgc ctttggcgtt ggcgcgttcc cagtaaagac ccagcaccag
     3961 cggccacagg aaaacggctt ccagcccacc gaaggccagc aaattcagcc agatgatcat
     4021 ttctggcggc ttccaggcgg caagcagcag caacgcgccg agaactaacg taattaccgc
     4081 cgacatccgc ttcagacgcg tctcgttttg catttgatcc ggacggatat tcagatagag
     4141 atctttaatg atcgtagcgg aactttgcag cagttgggcg ttaattgtcg acatgatcgc
     4201 agccataggt gcagccagaa agatcccggc agcaaaaggt ggcagcactt ttaccattaa
     4261 cgtcgggatc accagatccg gtacggtgag atcggggatc actgcacgac ctaacgctcc
     4321 ggccaggtgc ataccgaaca tcaggattgc gactataatc gtaccgatga tgatcccccg
     4381 gtgtactgct ttgctgtctt tataagagat acagcgcacc gcagtatgcg gcaggccaat
     4441 cacgccaaag cacaccagta cccagaacga cgtcataaag gcaggcgaca gaatatcgtc
     4501 agcgccttgt ggcgtaacca gttgcggatc gatggtttgc aaggtctgta ctgcgttgct
     4561 taagccgcca gcggcatgta ccacgccaat aagcagcaca acggtgccaa tcagcatcac
     4621 aagcccttgc atggtgtcgt tcagcacgct ggcgcgaaag ccaccaaagg cggtatataa
     4681 cgcaatgctg ataccaaaaa tcagcagccc ggtttcataa ggaatacccg ccgcggtttc
     4741 cagcaggcgc gcaccgccga taaactgcac ggtcatcgca ccaacgaacg caaccagcaa
     4801 actcaaactc gccagccaca ccagaagacg actctggtag cgggcaaaca gcatatcgtt
     4861 cagcgtcact gcattgtagc ggcgcgcaag aatcgcaaac ttcttgccga gaataccgag
     4921 tgaaagccag acagcaggaa gctgaatcat cgccagcaat acccagccca gcccgtattt
     4981 ataagcagct cctggcccgc cgataaacga actggcactg atataggtcg cggtgagcgt
     5041 catcgccagc acaataccgc ccatagagcg gctgccgagg aaatactcat taaggaaggt
     5101 gccggtgctc cgtttacgca tcgcataaac cgagataccg aacaccacca ccagataggc
     5161 gaccagcggt agaattactt caagctgcat cgtcatcctc cagtgggata tcgcgataga
     5221 taaatttcac catcgcccag cacagtccaa taaacagtag cggcgtcagg atgcaggcca
     5281 tctcaaacca gcgcggaaag ccggtaaaac cgggggcaac gccaggtaag taagcgtcta
     5341 ctaaccaaac tgccagatac aaaagggtca gccccagcgc ccagcgcgcc tctttatggg
     5401 cctgaacaaa acgagtgtcc attttttgtc cctgatgggt gaagaaagcg aggattgtac
     5461 cttatggggg ttgtcgatcc ccagtaataa aaaaggccgg aaaatccggc cttttgacgc
     5521 ttttgcagtc ttatttttcc tgaagaccga gttttttctc cagatagtgg atgttagtgc
     5581 caccatgctg gaagttctcg tcattcatga tgcggatctg cagatcaacg ttggttttga
     5641 taccgtcgat gatcagctcc tgcagcgcat tcttcatgcg ggcaatcgcc acgtcacggt
     5701 tttcaccgta gcaaatcagc ttaccgatca ttgagtcata gtacggcggt acggtgtagc
     5761 ccgcgtagat atgagactcc caacgtacgc caaaaccgcc aggtgcgtgg aaacgggtga
     5821 ttttgcctgg gcttggcagg aaggtgttcg gatcttcggc gttgatacga cattccaccg
     5881 catggccgcg aacgtgaact tcttcttgct tgatcgacag cggttgaccg gcagcgatac
     5941 gcagctgttc tttgatcagg tcaacgccgg tgatcatttc tgtaaccggg tgttctacct
     6001 gaatacgggt gttcatttcg atgaaataga actcgccgtt ttcgaacagg aactcgaaag
     6061 tacctgcacc gcgatagccg atatcaacac acgctttagc gcaacgtttg ccgatgtagc
     6121 gacgcagttc cggggtaatg cccggtgctg gtgcttcttc gaccactttc tggtggcggc
     6181 gctgcatgga gcagtcacgt tccgccagat agatagcgtt gccctgaccg tcagccagta
     6241 cctgaatctc gacgtggcga ggattctcca ggtatttctc catgtaaacc atatcgttgc
     6301 tgaaagcagc tttcgcttcc gcacgggtca tggagatgga ttgtgccagt tcagcgtcgc
     6361 cgcgcactac gcgcataccg cgaccgccgc cgccgccgga ggctttgata atcaccggat
     6421 aaccaatgcg tttagcaatg gcgcggtttt tatccatatc gtcgcccagc gggccgtcag
     6481 aaccaggtac gcaaggaacg cccgcttttt tcatcgccgc gattgcggat actttgtcgc
     6541 ccatcaggcg aatggtttct gctttcgggc cgatgaagat aaagccggag cgttcaacct
     6601 gctcggcaaa gttggcgttc tcggagagga agccgtaacc cggatggatt gctactgcgc
     6661 cggtgatttc agcggcgctg atgattgccg ggatgttcag ataacttttt actgacggag
     6721 cagggccaat acagaccgtt tcatctgcca gtaatacgtg ttttagatcg cgatccgtgc
     6781 tggagtgcac agcgacagtc ttgatgccca gttctttaca ggcacgaaga atacgcaatg
     6841 caatctcgcc gcggttggca ataacaattt tatccagcat gttcgcctcg ttactcgatg
     6901 acgaccagcg gctcgtcaaa ttctaccggt tgtccacttt cgaccagaat tgctttcacg
     6961 gtaccggatt tgtccgcttc gatctggttc atcattttca tggcttcaac gatgcacagg
     7021 gtatcgccca cgttgacttt ctgacccact tcgatgaacg cttttgcgtc cgggcttggg
     7081 gtgcggtaga aagtaccaac catcggggaa cgtacgatgt gaccactgat ttccgctgct
     7141 gctggcgctt ccatggaagg aacggtcgcc ggagcggctg cgttagattg agctggctgc
     7201 tgcatcattg gtgcagcgta agcttgttgc atcacaggga aacttgcggc aggagctgca
     7261 cggctaatgc gtactgactc ttcgccttca gaaatttcca gttcggagat gcctgattct
     7321 tcaaccagct cgatcagttt tttaatctta cgaatatcca tgagtgggtt ccgtactctt
     7381 tgtttagtgt gattgtgaca ggcgtttcac cgccgtctgt aaagcgtatg ccccgacagc
     7441 tgtatgcata gcgataaatt ccagcaggcc gggacgccgg tctattttgc cccagagtgt
     7501 caatgttaga cttgacggac attgtgcagc gatctgctgc tatcctccgg caaaaaacaa
     7561 aatatacctt cgttgcgcca atgtcacctt ttccgcagcg caaaaactgc gtcagggagg
     7621 acgaggtcgc acattataac gatttcgtag caattggcag ctaaatactg gtcttatcag
     7681 ggaatataat caacagctaa catgtaaata accttcaaca ccgtgtaatt tgcaacaagc
     7741 cgcacaattc acgaaattag cgccatcatc gacggaactt cttataacgt aaggtgaaaa
     7801 gctgaaaagc cagccctgcg aagatgaggg gctgcaatga gataatcttc acagaccaca
     7861 aataatgtag ggatgccagg atcgcgacaa gatagacgac attacgcagc ggttgctggg
     7921 gattacccaa ttttcactgc atcgtctgga tagatgtgaa tgctaaagca cgcaaaatca
     7981 accaactgac aattctcagc attaaatagg ttcgagtggt taatttgtag tcaaataaaa
     8041 tcagattatt caccccatgg taccagaaat cagtaacagg ttaagcgtag tgtcgcccag
     8101 gcaaagcacc ataaacctaa caggcgacgg aggcgtatca ataatggctg tttagaggag
     8161 tatcaatgcg catcaatttg tggtcagtcg aaatcatgac tttccagttt ggatgatgtt
     8221 gtacaacttc ctgaccaagg tgttcgacga aagctgtccg gctaaccgct tttcccgtta
     8281 accgattgaa taatgatgtc aaaatgagtt ccctctctta ttattccttg ctaaatggtt
     8341 agttaacctt caccagcgtg cgaccctgga tctggttatt aatgatggcc tcggcaaagt
     8401 tcggtgcctc tgacagagat atctcttttg ccgcctgggt atagaatgat tccggtaaat
     8461 cggcgaccag tcgctgccag gcttgtgcgc ggcgttctgg tggcgtcatt actgaatcca
     8521 ccccttgcaa acggacatta cgcagaataa atggcatgac cgtggttggc agagtaaaac
     8581 cacccgccag accacaggcc gccacgcagc cgccgtaatt catttgcgcc agcacttttg
     8641 ccagcacttt gtcgccaaca gtgtcaattg ccccagccca gacttgtttt tccagaggac
     8701 gggattcggc aaactcatca cgagggagaa taccgctggc acctaaactt ttcagatatt
     8761 catgggtact ttcgcgaccg gaaacggcaa cgacctgata acccaactta tgcagcagcg
     8821 ccacggcggt actgccgacg ccaccactgg cacccgtcac gacaatctcc ccgtcctgcg
     8881 ggcgaacacc ggcatcttcc agcgccatca cacacagcat ggcggtaaaa ccggcagtac
     8941 cgataatcat tgctttacgc gcgtccagcc cttgcggcat ggcaaccagc cagtcacctt
     9001 tcactcgcgc ctgctccgcc agcccacccc agtggttttc accaacgccc cagccagtga
     9061 gtaacacctc ctgaccggca tgaaaacgcg gatcttcgct ggtgcgtaca gttccggcaa
     9121 aatcgatccc aggaatcatc ggaaaattac ggatgatttt tcccttaccg gtaatcgcca
     9181 gcgcatcttt atagttcagg ctcgaccagt gaacatcgac cgtgacatcg ccctccggca
     9241 ggcgacgttc gtccagagtc tgtactgatg cgagagtttt gccgtcctgc tgttctaaaa
     9301 gtaacgcctg cataagtggt cctcatgtgc atgatggatt ggaaaataat ttctgaagac
     9361 tatactcgct aatggaaaag caaagccgat gaagcgcaat aaattgcgag ataaatctga
     9421 tttgctagta tgcccgcttc ctcactatcg gagttaacac aaggatgaga ttaacgacga
     9481 aattttcggc ctttgttacg ctgctcaccg ggttaacaat ttttgtgact ttgctgggct
     9541 gttcgctaag tttctacaac gccattcagt ataagtttag tcatcgcgtt caggcggtgg
     9601 cgacggcgat tgatacccac cttgtgtcga atgacttcag cgcattaagg ccacaaatta
     9661 ccgaattaat ggtgtcagca gatatcgttc gtgtagacct gctgcatggt gataaacagg
     9721 tttataccct ggccagaaat ggtagttatc gtccggttgg caccagcgat ctgtttcgtg
     9781 aactgagcgt tccgttgata aagcatccgg ggatgtcgtt gcgtctggtt tatcaggatc
     9841 cgatgggcaa ctatttccat tcgttgatga ccaccgcgcc gctcacgggg gcgattggct
     9901 ttatcattct tatgctcttt ctggcggtac gctggttaca acggcaactt gccgggcaag
     9961 aattgctgga aacccgggct actcgtatct taaacggtga gcgtggctct aatgtgttgg
    10021 gaaccatcta tgaatggccg cccagaacca gcagtgcgct ggatacgctg cttcgtgaaa
    10081 ttcagaacgc acgcgaacaa cacagccgtc ttgatacgct gatccgctct tatgccgcac
    10141 aggacgtgaa aaccggcctc aataaccgac tctttttcga taatcagtta gcaacgttac
    10201 tggaagatca ggagaaagta ggtacccacg ggatcgtgat gatgattcgt ctgccggatt
    10261 tcaatatgtt gagcgatacc tgggggcaca gccaggttga agaacagttc ttcactctga
    10321 cgaatctgct gtcgacattt atgatgcgct accctggcgc actgctggcg cgttaccacc
    10381 gcagtgattt tgctgcgctg ttaccgcacc ggacgttaaa agaggcagag agcatagcca
    10441 gtcagttaat caaagccgtc gataccttgc cgaacaataa aatgctcgat cgcgacgata
    10501 tgatccacat tggtatctgt gcctggcgta gtggtcagga taccgagcag gtaatggaac
    10561 atgcagagtc tgccacgcgt aatgcgggat tgcagggtgg caatagctgg gctatttatg
    10621 atgactcgtt gcctgaaaaa ggacgcggta atgttcgctg gcgtacgctt atcgagcaaa
    10681 tgctcagtcg cggcggcccg cgcctttatc aaaaaccggc ggttactcgc gaaggtcagg
    10741 ttcatcatcg cgaactcatg tgccgcatct tcgatggtaa tgaagaggtt agctcggcgg
    10801 agtatatgcc gatggtcttg cagtttggct tatcggaaga gtatgaccgt ctgcaaatca
    10861 gccgtcttat tccactattg cgttactggc cagaggaaaa tctggcgatt caggttaccg
    10921 ttgagtcgct gattcgtccg cgttttcagc gttggctgcg cgatacgtta atgcaatgtg
    10981 aaaaatcaca acgaaaacgc ataattattg aacttgcaga ggccgatgta ggtcaacata
    11041 tcagtcgttt acaacctgtt attcgtttag tgaatgcttt aggggtacgg gtagccgtca
    11101 accaggctgg tttgacgctg gtaagcacca gttggatcaa agaacttaat gttgagttac
    11161 tcaagctcca tccggggctg gtcaggaaca ttgagaagcg aacggagaac cagctgctgg
    11221 ttcaaagcct ggtggaagcc tgctccggga ccagcaccca cgtttacgcc accggcgtgc
    11281 gttcgcgaag cgagtggcag accctgattc agcgcggtgt tacaggcggg caaggggatt
    11341 ttttcgcgtc ctcacagcca cttgatacta acgtgaaaaa atattcacaa agatactcgg
    11401 tttaacctgc cgtttaatcc gttttcacgt agaataacgc gcgctgcgtc tcatgggagt
    11461 gtgcttgtct gctcgccaga ttgttgcagc acatatgcag atgaatgacc ttacgcggtt
    11521 gcaaacaggc gaggaatgct gctgatgcat taagcctttc tggactcagg cagagatttg
    11581 taacaaagga aacgaactgc actaattttc accgtagcag atgatttttg cgccttgtcg
    11641 ctgctgcgtg tggttggtaa agtaagcgga ttttcttttc cgccccagct ttcaggatta
    11701 tcccttagta tgttgaaaaa atttcgtggc atgttttcca atgacttgtc cattgacctg
    11761 ggtactgcga ataccctcat ttatgtaaaa ggacaaggca tcgtattgaa tgagccttcc
    11821 gtggtggcca ttcgtcagga tcgtgccggt tcaccgaaaa gcgtagctgc agtaggtcat
    11881 gacgcgaagc agatgctggg ccgtacgccg ggcaatattg ctgccattcg cccaatgaaa
    11941 gacggcgtta tcgccgactt cttcgtgact gaaaaaatgc tccagcactt catcaaacaa
    12001 gtgcacagca acagctttat gcgtccaagc ccgcgcgttc tggtttgtgt accggttggc
    12061 gcgacccagg ttgaacgccg cgcaattcgt gaatccgcgc agggcgctgg tgcccgtgaa
    12121 gtcttcctga ttgaagaacc gatggctgcc gcaattggtg ctggcctgcc ggtttctgaa
    12181 gcgaccggtt ctatggtggt tgatatcggt ggtggtacca ctgaagttgc tgttatctcc
    12241 ttgaacggcg tggtttactc ctcttccgtg cgcattggtg gtgatcgttt cgacgaagct
    12301 atcatcaact atgtgcgtcg taattacggt tctctgatcg gtgaagccac cgcagaacgt
    12361 atcaagcacg aaatcggttc ggcttacccg ggcgatgaag tccgtgaaat cgaagttcgt
    12421 ggccgtaacc tggcagaagg tgttccacgc ggttttaccc tgaactccaa cgaaatcctc
    12481 gaagcactgc aggaaccgct gaccggtatt gtgagcgcgg taatggttgc actggaacag
    12541 tgcccgccgg aactggcttc cgacatctcc gagcgcggca tggtgctcac cggtggtggc
    12601 gcactgctgc gtaaccttga ccgtttgtta atggaagaaa ccggcattcc agtcgttgtt
    12661 gctgaagacc cgctgacctg tgtggcgcgc ggtggcggca aagcgctgga aatgatcgac
    12721 atgcacggcg gcgacctgtt cagcgaagag taatcggatg caggcagggg aagcgtctgt
    12781 ttaccctgcc tggtctgata cgagaatacg cataacttat gaagccaatt tttagccgtg
    12841 gcccgtcgct acagattcgc cttattctgg cggtgctggt ggcgctcggc attattattg
    12901 ccgacagccg cctggggacg ttcagtcaaa tccgtactta tatggatacc gccgtcagtc
    12961 ctttctactt tgtttccaat gctcctcgtg aattgctgga tggcatatcg cagacgctgg
    13021 cctcgcgtga ccaactagaa cttgaaaacc gggcgttacg tcaggaactg ttgctgaaaa
    13081 acagtgaact gctgatgctt ggacaataca aacaggagaa cgcgcgtctg cgcgagctgc
    13141 tgggttcccc gctgcgtcag gatgagcaga aaatggtgac tcaggttatc tccacggtta
    13201 acgatcctta tagcgatcaa gttgttatcg ataaaggtag cgttaatggc gtttatgaag
    13261 gccagccggt catcagcgac aaaggtgttg ttggtcaggt ggtggccgtc gctaaactga
    13321 ccagtcgcgt gctgctgatt tgtgatgcga cccacgcgct gccaatccag gtgctgcgca
    13381 acgatatccg cgtaattgca gccggtaacg gttgtacgga tgatttgcag cttgagcatc
    13441 tgccagcgaa tacggatatt cgtgttggtg atgtgctggt gacctccggt ctgggcggtc
    13501 gtttcccgga aggctatccg gtcgcggttg tctcttccgt aaaactcgat acccagcgcg
    13561 cttatactgt gatccaggcg cgtccgactg caggactgca acgtttgcgt tatctgctgc
    13621 tgctgtgggg cgcggatcgt aacggcgcta acccgatgac gccggaagag gtgcatcgtg
    13681 ttgctaatga acgtctgatg cagatgatgc cgcaggtatt gccttcgcca gacgcgatgg
    13741 ggccaaagtt acctgaaccg gcaacgggga tcgctcagcc gactccgcag caaccggcga
    13801 caggaaatgc agctactgcg cctgttgcgc cgacacagcc cgctgctaat cgctctccac
    13861 aaagggctac gccgccgcaa agtggtgctc aaccgcctgc gcgtgcgccg ggagggcaat
    13921 agtggcgagc tatcgtagcc agggacgctg ggtaatctgg ctctctttcc tcattgcgct
    13981 gttgctgcaa atcatgccct ggccggataa cctgattgtt ttccggccaa actgggtgtt
    14041 acttatcttg ttgtattgga tcctggcctt gccccatcgc gtaaatgtgg gcacaggttt
    14101 tgtgatgggt gccatactgg atctgatcag cggctcgatg cttggcgtac gcgtattggc
    14161 gatgagcatc attgcttacc tggtggcgct gaaataccag cttttccgca acctcgcatt
    14221 atggcagcag gcgctggtcg tcatgttgct ttcgctggtg gtggatatta ttgttttctg
    14281 ggcagagttt ttagtgatta acgtctcttt cagaccggaa gtgttctgga gtagtgtagt
    14341 caatggggtg ctctggccgt ggattttctt gctgatgcgt aaagtccgtc agcagtttgc
    14401 agtgcaataa aggtttctat gacttctctg tatttagctt ccggttctcc gcgtcgtcag
    14461 gagttacttg cgcaacttgg cgtgaccttt gaacgtattg ttacgggcat tgaggagcag
    14521 cgtcagccgc aggagagcgc gcagcagtat gttgtgcgtc tggcacgcga gaaagcacag
    14581 gcgggtgtcg cgcaaacggc acaggatctc ccggtgctgg gtgcggatat tatcgttatc
    14641 ctgaacggag aagtgctcga gaaaccgcgc gacgcagagc atgcggcgca gatgttgcgc
    14701 aaattatcgg gtcagaccca tcaggtgatg acggcagtgg cgttggccga cagccagcac
    14761 attctcgatt gtctggtggt caccgatgtg actttcagaa cgttaacaga cgaagacatc
    14821 gcgggctatg tcgccagcgg tgaaccgtta gataaagcag gtgcatacgg tattcagggg
    14881 ctgggtggct gttttgtcag gaagataaat ggcagctatc acgccgtagt cggcttaccg
    14941 ctggttgaaa cgtatgaatt attaagtaat tttaacgcac tgcgtgagaa aagggataaa
    15001 catgacggct gaattgttag taaacgtaac gccttcggaa acgcgagtgg cgtatattga
    15061 tggcggtatt ctgcaggaaa ttcatattga acgtgaggcg cgacgcggaa tagtaggcaa
    15121 tatctacaag ggtcgtgtaa gtcgtgtact tccgggtatg caggcggctt ttgtagatat
    15181 tgggctggat aaagccgcgt ttcttcatgc atccgacatc atgccgcata ccgaatgtgt
    15241 ggcgggtgaa gaacaaaagc aattcacggt gcgcgacatc tcggaactgg ttcgtcaggg
    15301 gcaagatctg atggtgcagg tggtgaaaga tccgcttggc actaaaggtg cgcgcctgac
    15361 caccgatatc acgctgcctt ctcgctatct ggtatttatg ccaggggctt ctcacgttgg
    15421 ggtttcccaa cgaattgaaa gcgaatcaga acgtgaacgt ctgaaaaaag tggtcgcaga
    15481 gtattgcgac gagcagggcg ggtttatcat ccgcaccgca gcggaagggg ttggcgaggc
    15541 tgaactggcc tccgatgccg cttatctgaa acgcgtctgg accaaagtta tggagcgtaa
    15601 aaagcgcccg cagacccgtt atcagctgta cggcgaactg gcgctggcgc agcgtgttct
    15661 gcgtgatttt gccgacgccg aactggaccg cattcgcgtt gactcacgcc tgacttacga
    15721 agcattgctg gagtttacct cggagtacat tcccgagatg accagcaagc tggagcatta
    15781 caccggacgc cagccgattt tcgatctctt tgatgtcgaa aacgaaatcc agcgagcgtt
    15841 ggaacgcaaa gtagaactga aatccggtgg ttatctgatt atcgaccaga ccgaagcgat
    15901 gaccaccgtg gacatcaata ccggagcgtt tgtcggtcat cgcaatctgg acgacactat
    15961 tttcaatacc aatattgaag cgacgcaggc tatcgctcgc cagttacggt tgcgtaatct
    16021 gggcgggatt atcattattg atttcatcga tatgaataat gaagatcacc gccgccgcgt
    16081 gctgcattcg ctggagcagg cgttgagcaa agaccgggtg aaaaccagcg ttaatggttt
    16141 ttcggcgctg gggctggtgg agatgacgcg taaacgcacc cgcgaaagca ttgagcacgt
    16201 actgtgtaac gaatgcccaa cctgccacgg tcgcggaacg gtgaaaaccg tggaaacggt
    16261 atgctatgaa atcatgcgcg agattgttcg tgtccaccat gcttacgact ccgaccgttt
    16321 cctggtctat gcttctccgg cagtagccga agccttgaaa ggcgaagagt cacactcgct
    16381 ggcggaagtg gaaattttcg ttggcaaaca ggttaaagta caaattgaac cgctctataa
    16441 ccaggagcag tttgacgtcg taatgatgta aacagatgct gggccgccat ccggcaaagg
    16501 gtttttgagt cacattttta gcagacaagg agtgacgggt gaggcgattg ccggggattt
    16561 tactgcttac tggagccgcg ctcgttgtga tcgctgccct gctggttagc ggcctgcgta
    16621 ttgctttacc gcatcttaac gcctggcgtc cggaaatcct caacaaaata gaatccgcga
    16681 ctggcatgcc ggtagaagcc agtcagctct cagccagctg gcagaatttt ggcccgacgc
    16741 ttgaagcaca cgacatccgt gcagaactaa aagatggcgg cgaattttcg gttaaacgcg
    16801 ttactctggc gctggatgtc tggcagagcc tgttacatat gcgctggcag tttcgcgacc
    16861 tcactttctg gcagctgcgc tttcgcacca acactcctat caccagcggt ggtagcgatg
    16921 acagtctgga agccagtcac atcagcgatc tgtttcttcg tcaatttgac catttcgatc
    16981 tccgcgacag tgaagtcagt tttctgacgc catccggtca gcgcgccgag ctggcgatcc
    17041 cacaactcac ctggctgaac gatccacgtc gacaccgtgc ggaaggcctg gtaagcctct
    17101 ccagccttac cggacagcac ggcgtgatgc aggtgcgcat ggatttgcgc gatgatgagg
    17161 ggttgttaag caatggtcgc gtctggctac aggcggatga catcgacctg aagccgtggc
    17221 tcggtaaatg gatgcaggac aatattgctc tggaaacggc acagttctcc cttgaaggct
    17281 ggatgacgat cgacaaaggc gatgtaaccg gcggtgacgt ctggctgaaa cagggcggtg
    17341 ccagctggtt gggcgagaag caaacgcata cgctgtcggt ggataatctg accgcgcata
    17401 ttacgcgtga aaatccgggc tggcagttct ctattcccga tacacggatc acgatggacg
    17461 gcaaaccctg gccgagcgga gcattgacgc tggcctggat accggaacag gacgttggcg
    17521 gcaaagacaa taaacgcagt gacgaactcc ggattcgcgc tagtaatctg gagctggcag
    17581 gcctggaggg catacgcccg ctggccgcga aactttcacc tgcactgggt gatgtttggc
    17641 gctccacaca accgagcggc aagattaaca ctctggcgct ggatatcccg cttcaggcgg
    17701 caggcaagac ccgttttcag gcatcgtgga gcgatctggc ctggaagcaa tggaaattat
    17761 taccgggtgc ggaacacttc tccgggacgc tttccggcag cgttgaaaat ggtttgctta
    17821 ccgcgtcgat gaagcaggca aagatgcctt acgaaacggt attccgtgcg ccactggaaa
    17881 tcgccgacgg ccaggcaact ataagctggc tgaacaatga caaaggtttc cagctggatg
    17941 ggcgtaatat tgacgttaaa gccaaagccg tccatgcgcg cggcggtttt cgttacctgc
    18001 aacctgctaa cgatgaaccc tggctgggta ttctggctgg catcagtacc gatgatggtt
    18061 cacaagcctg gcgctatttc ccggaaaact tgatgggtaa agacttggtt gattatttaa
    18121 gtggtgcgat tcagggcggt gaagcggata acgcgacgct ggtttatggt ggcaatccgc
    18181 aactcttccc ctataaacac aacgaaggtc agtttgaagt gctggtgccg ctgcgcaacg
    18241 cgaagtttgc cttccagccg gactggcctg cattaactaa ccttgatatt gaactggact
    18301 ttattaacga cggtttatgg atgaaaaccg atggcgttaa tctgggcggc gtgcgcgcga
    18361 gtaatctcac cgcagtgatc cctgactact caaaagaaaa actgctgatt gacgctgaca
    18421 ttaaaggtcc gggtaaagcc gttggccctt actttgatga gacaccgctg aaagattctc
    18481 tgggtgcgac cctgcaagaa ctccagctcg acggcgatgt gaatgctcgc ttacatcttg
    18541 atatcccgct gaacggcgaa ctggtaaccg cgaaaggtga agtgacgctg cgtaataaca
    18601 gtctgtttat caaaccactc gacagcatcc tgaaaaattt gagcggtaaa ttcagcttta
    18661 tcaatggcga tctgcaaagt gaaccactga cagcaagctg gtttaatcag ccgttgaacg
    18721 tggatttttc caccaaagaa ggggcaaaag cctaccaggt tgcggtgaat ctcaacggta
    18781 actggcaacc ggcgaaaacc ggcgttctgc ctgaagcggt gaacgaagca ttgagtggca
    18841 gcgtggcgtg ggatggtaaa gtgggcattg atctgcctta tcatgctggc gcgacctata
    18901 acgtagagct gaacggcgat ctgaataatg tgagtagtca cttaccttca ccgttagcca
    18961 aacctgcggg tgaaccacta gcggtaaacg ttaaggttga tggcaatctc aacagctttg
    19021 aattaaccgg acaggctggt gcggataacc atttcaatag ccgctggttg cttggtcaaa
    19081 agctgacgct cgatcgtgct atttgggcgg cagacagtaa aacgctcccg ccgttgccgg
    19141 aacaaagtgg cgttgaactt aatatgccgc cgatgaatgg tgccgagtgg ctggccctgt
    19201 tccagaaagg cgctgcggag agtgtcggtg gtgcagcgag tttcccacaa cacataacgt
    19261 tacgtacgcc tatgttgtca ctgggaaatc agcaatggaa taacctgagt attgtttcgc
    19321 aaccgacggc aaatggcacc ctggttgagg cgcaagggcg tgaaatcaac gccacgctag
    19381 cgatgcgtaa taacgcgccg tggctggcga atatcaaata tctttattac aacccgagcg
    19441 tggcgaaaac tcgtggtgat tcaacgccgt catcaccttt cccgacaacg gagcgcatta
    19501 acttccgtgg ctggccggac gcacaaatac gatgcacaga gtgctggttc tgggggcaaa
    19561 aattcggtcg cattgacagt gatatcacca tttctggcga tacgttaacg ctgaccaatg
    19621 gactgattga tactggtttc tcgcggctta ctgccgatgg tgaatgggtt aataatccgg
    19681 ggaatgaacg tacctcgctg aaaggaaaac tgcgtgggca gaaaattgat gccgccgcag
    19741 aattttttgg tgtcacgacg cccatacgcc agtcgtcatt taatgtggat tacgatttac
    19801 actggcgcaa agcaccctgg cagccagatg aagcgacgtt gaatggcatc attcatactc
    19861 aactgggtaa aggcgaaatt accgaaatca ataccggaca tgccgggcaa ttgctgcgct
    19921 tattgagcgt agatgccctg atgcgtaagc tgcgttttga tttcagagac acttttggcg
    19981 aagggttcta ttttgactcc attcgcagca ccgcgtggat taaagacggc gttatgcaca
    20041 ccgacgacac gctggtggat ggcctggagg cggatatcgc catgaaaggg tcggtaaatc
    20101 tggtacgccg cgacctgaat atggaagcgg ttgtcgcacc agagatttct gcgacggtgg
    20161 gcgtggctgc ggcttttgcg gttaacccca ttgttggcgc ggcagtgttt gccgccagta
    20221 aagtgctggg gccgctgtgg agcaaagtct ccattttgcg ctatcacatt tcgggtccgc
    20281 tggacgatcc gcaaatcaac gaagtgttgc gccaaccgcg taaagaaaaa gcgcaatgat
    20341 ttgacgaggg cgcgtaattg ccccaatctc atatgataat cgttgccaaa ggccaacgag
    20401 ccggaacata actgtaggcc ggatagggcg ttcacgccgc atccggcagc cgtaaaaaat
    20461 cctctactgc agtaactaac gagtagcaaa aacgatgagt ctgaacctgg taagtgaaca
    20521 attgctagcg gcgaacggcc tgaaacatca ggacttgttc gcgatcctcg gtcaactggc
    20581 cgaacgtcgc cttgattatg gcgatctcta ttttcagtcg agctatcacg aatcctgggt
    20641 tttagaagac cgcattatta aagatggttc ttacaacatc gatcagggcg ttggcgtgcg
    20701 tgcaatcagc ggtgaaaaaa ccggatttgc ttacgctgac caaatcagcc tgctggcgct
    20761 ggaacagagt gcgcaagcgg cgcgcaccat cgtccgtgat agtggtgatg gcaaagtaca
    20821 gacgctgggc gcggtagagc atagcccgtt gtatacctcg gtagatccgc tgcaaagcat
    20881 gagccgtgaa gagaagctgg atatcctgcg tcgcgtcgat aaggttgctc gcgaagcgga
    20941 caagcgcgta caggaagtga ctgccagcct cagtggtgtc tatgaattaa ttctggttgc
    21001 ggccaccgac ggcacgctag cggcggatgt ccgtccgctg gtgcgtcttt ccgtgagcgt
    21061 tctggtcgaa gaagatggca aacgcgaacg cggtgccagt ggcggcggcg gtcgttttgg
    21121 ttatgagttc ttccttgccg atcttgacgg cgaagtccgt gcggatgcat gggcaaaaga
    21181 agcagtgcgt atggcgctgg tcaatctttc tgccgttgct gcaccagcgg gcaccatgcc
    21241 ggtagtactt ggcgcaggtt ggccgggcgt gctgttgcat gaagcggtag gtcacggtct
    21301 ggaaggcgac ttcaaccgcc gtggcacttc agtatttagt ggacaggtcg gggagctggt
    21361 ggcttcagaa ctgtgtaccg tggttgatga cggcacgatg gtcgatcgcc gtggttcggt
    21421 ggcgattgat gatgaaggta cgccaggcca gtacaacgtg ctgattgaga acggcattct
    21481 gaaaggctac atgcaggata aactcaacgc gcgtttgatg gggatgacgc cgactggcaa
    21541 cggtcgccgt gaatcctacg cccatctgcc catgccgcgt atgaccaaca cctatatgct
    21601 gccgggtaaa tcgaccccgc aagaaattat tgaatccgtt gagtacggta tctatgcacc
    21661 gaactttggt ggcggtcagg tggatatcac ctccggcaaa ttcgttttct ccacttcaga
    21721 agcatatctg attgaaaacg gtaaagtaac gaagccggtg aaaggcgcaa cgttgattgg
    21781 ttccggtatc gaaaccatgc agcagatttc gatggttggc aacgacctga aactggataa
    21841 cggcgtgggt gtctgcggta aagaagggca aagtttgccg gttggcgtgg gccagccaac
    21901 gttgaaagtc gataacctga ctgttggcgg tactgcgtaa taatttcatt attttcagaa
    21961 ggataattaa tctttctacg tgcacgaacg gtcccctcgc tcctctgggg ttagggtgag
    22021 gggaacccgt tggcacaggt ttgtagaacg taacagtaca atatgaatta cttctctttc
    22081 ccgcgcccgt gcatctcctg aaacaattta ccgacctcaa caaaataatc cgtcagcgag
    22141 ttgatcacga cctgtacctt cagcggcagc ttatcttttt cggtatataa cgcataaacc
    22201 gggcgtggat ctgactgata acgcggcagc aggatctcca gctccccacg attgatctcg
    22261 ttgatcaccc acatcagcgg cacgtaggcg atcccggcac ccgccgttag ccagcgcacc
    22321 agcgtcatcg gatcattagt cacaaatctc ccttgcggga tcaggcgagt cgagatccct
    22381 tccggtgcaa tcagttcaaa ttcattgtcg ggccgcacgc tgtattcaag ccatgaatgg
    22441 ctacttaaat cggcgggttt ttccggtatg ccgtattgtg tgagatagct tttcgcggcg
    22501 cacaccacca ttggcatcgc gccaagacgg cgggaaaaca ggctggaatc ctgcaacgcg
    22561 ccgacgcgga tcaccacatc cagaccgtcg gcaatcaggt cgggggctgg aattccggta
    22621 accagattgg cgctcaaacc tgggtattct ttcagcattt tggctgtcag cccggcgaga
    22681 acattttgtg ccatagttga agaacagcca atgcgtagcg tcccgatggg ggtgttattg
    22741 aaggcataca gttgctcatg aacatcctgc acttcatgaa gcatacgacg gcagccctgg
    22801 tagtaaattc taccggcttc ggtcaggcca atgctgcgtg tgctacggtt taacagcttt
    22861 acctgcaact catcttccag ttttgacacc gtctgactga tggacgaaac gctcatctgt
    22921 agctgtctgg cggcggcggt aaaagagcca aattcaacta ctttggcaaa caccgacatg
    22981 cgttttagtc gttccattat tcactctgac ttaaaagtga tttagatcac ataatataga
    23041 taacagcata acagttacgc taatatatta aatatcaatc tacagcaatg ttgctctcgc
    23101 ccggctttcc atgcccttct ctgcggcgac agatgctgaa aataataacg cctgctctct
    23161 ctatacaacc aaggtcaaca tgagtctgtt tcccgttatc gtggtgtttg ggctgtcctt
    23221 cccaccgata ttttttgaat tgcttttatc actggcgatt ttctggctgg tgcgccgggt
    23281 acttgtgcca acaggtatct acgactttgt ctggcatccg gcgttgttca acaccgcgct
    23341 ctattgctgc ttgttttatt tgatatcgcg actgttcgtt tgaggttgaa gtgaaaacac
    23401 taataagaaa attctcccgt acggccatca cggtcgtatt agtcattctg gccttcatcg
    23461 caatttttaa tgcctgggtc tattacaccg aatccccctg gacgcgtgac gcgcgcttta
    23521 gcgctgacgt cgttgcgatc gcgccggacg tttctggact cattacccag gtgaatgttc
    23581 atgataacca gctggtgaaa aaaggacaga tactgttcac catcgaccag ccgcgctatc
    23641 aaaaggcgct tgaggaagcg caagccgatg ttgcttatta tcaggtactg gcacaggaga
    23701 aacgccagga ggccggacgt cgtaaccgtc tcggtgtgca ggcgatgtct cgcgaagaga
    23761 tcgaccaggc taacaacgta ctacaaacgg ttctgcatca gttagcgaaa gcgcaggcga
    23821 cccgcgatct ggcaaaactg gatcttgaac gcacggtgat ccgagcgcca gcagatggct
    23881 gggtgaccaa cctcaacgtc tataccggtg agtttattac tcgaggatca acggcggttg
    23941 cgctggtgaa acagaactcc ttctatgtac tggcctatat ggaagaaact aagctggaag
    24001 gggtgcgtcc ggggtatcgt gcagagatca cgccgcttgg cagtaacaaa gtgctgaaag
    24061 ggactgttga tagtgttgcc gctggggtca ccaacgccag cagcacgcgt gacgacaaag
    24121 ggatggcgac catcgactct gaccttgaat gggtgcgttt ggcgcaacgt gttccggttc
    24181 gtattcgtct cgacaaccag caagagaaca tctggcctgc gggcaccact gctacagtgg
    24241 tggtcactgg caaacaagat cgcgacgaaa gccaggattc gttcttccgt aaaatggccc
    24301 atcgcctgcg tgagtttggt taatcacgat gggtattttc tccattgcta accaacatat
    24361 tcgctttgcg gtaaaactgg cgaccgccat tgtactggcg ctgtttgttg gctttcactt
    24421 ccagctggaa acgccacgct gggcggtact gacagcggcg attgttgccg ccggtccggc
    24481 ctttgctgcg ggaggtgaac cgtattctgg cgctattcgc tatcgtggct ttttgcgcat
    24541 catcggcaca tttattggct gtattgccgg actggtgatc atcattgcga tgatccgcgc
    24601 accattattg atgattctgg tgtgctgtat ctgggccggt ttttgtacct ggatatcctc
    24661 gctggtacga atagaaaact cgtacgcgtg ggggctggcc ggttataccg cgctgatcat
    24721 tgtgatcacc attcagccgg aaccattgct tacgccgcag tttgccgtcg aacgttgtag
    24781 cgagatcgtt atcggtattg tgtgtgcgat tatggcggat ttgctctttt ctccgcgatc
    24841 gatcaaacaa gaagtagatc gagagctgga aagtttgctg gtcgcgcaat atcaattaat
    24901 gcaactctgt atcaagcatg gcgatggtga agttgtcgat aaagcctggg gcgacctggt
    24961 gcgacgcacc acggcactac aaggcatgcg cagcaacctg aatatggaat cttcccgctg
    25021 ggcgcgggcc aatcgacgtt taaaagcgat caatacgcta tcgctgacgc tgattaccca
    25081 atcctgcgaa acttatctta ttcagaatac gcgcccggaa ttgatcactg atactttccg
    25141 cgaatttttt gacacgccgg tagaaaccgc gcaggacgtc cacaagcagc tcaaacgcct
    25201 gcggagagtt atcgcctgga ccggggaacg ggaaacgcct gtcaccattt atagctgggt
    25261 cgcggcggca acgcgttatc agcttctcaa gcgcggcgtt atcagtaaca caaaaatcaa
    25321 cgccaccgaa gaagagatcc tgcaaggcga accggaagta aaagtagagt cagccgaacg
    25381 tcatcatgca atggttaact tctggcgaac cacactttcc tgcattctgg gcacgctttt
    25441 ctggctgtgg acgggctgga cttccggcag tggtgcaatg gtgatgattg cggtcgtgac
    25501 gtcactggca atgcgtttgc cgaatccacg catggtggcg atcgacttta tctatgggac
    25561 gctggccgcg ctgccgttag ggctgctcta ctttttggtg attatcccta atacccaaca
    25621 gagcatgttg ctgctgtgta ttagcctggc agtgctggga ttcttcctcg gtatagaagt
    25681 acagaaacgg cgactgggct cgatgggggc actggccagc accataaata ttatcgtgct
    25741 ggataacccg atgactttcc atttcagtca gtttctcgac agcgcattag ggcaaatcgt
    25801 cggctgtgtg ctcgcgttca ccgttatttt gctggtgcgg gataaatcgc gcgacaggac
    25861 tggacgtgta ctgcttaatc agtttgtttc tgccgctgtt tccgcgatga ctaccaatgt
    25921 ggcacgtcgt aaagagaacc acctcccggc actttatcag cagctgtttt tgctgatgaa
    25981 taagttccca ggggatttgc cgaaatttcg cctggcgctg acgatgatta tcgcgcacca
    26041 gcgcctgcgt gatgcgccga tcccggttaa cgaggattta tcggcgtttc accgacaaat
    26101 gcgccgcaca gcagaccatg tgatatctgc ccgtagcgat gataaacgtc gtcggtactt
    26161 tggccagttg ctggaagaac tggaaatcta ccaggaaaag ctacgcatct ggcaagcgcc
    26221 accgcaggtg acggaaccgg ttcatcggct ggcggggatg ctccataagt atcaacatgc
    26281 gttgaccgat agttaagtca aaaccgacgc caaaagcgtc ggttttttca tggctatact
    26341 tagcgatgca cggcagaact cgccgcgaaa cgtgacggtg gcaacagatg aatatttatg
    26401 cctttgattt tgatgagatt gagagtcagg aggattttta tcgtgacttt agccaaacct
    26461 ttggtctggc taaagataag gtacgcgatc tcgactcact atgggatgtg ttaatgaacg
    26521 atgtcctgcc gctaccactt gagattgaat ttgttcatct gggagagaaa acgcgtcgcc
    26581 gttttggcgc gttaatattg ctgtttgatg aggcagagga agagctggaa gggcatttgc
    26641 gttttaatgt tcgtcattag cgcaaaaaaa agcccacgaa ccgggggcaa tatcgtcgga
    26701 caaaacgatg agggtttatt tgtacagttc agccgtagcg tgccaggtgt caccgctacg
    26761 agcttcagta atctggtagg ccgttgcgcc tttctcttcc gcttttttgt tcagcatttc
    26821 acgtatatcc attggcgaag acgccacacc acttacggat acggtcccga ttgcttcacg
    26881 attttgtgct tgtgcagcat caatggagtc ggcagcgaat gcaccgaaag agagaacaga
    26941 aagtacgctt aatgcagcta cagtggtttt gattttcatg atttttacct cgacataatc
    27001 ttttagctgg gcctttgttt cgtgacccat atcacaaaat caagtataca ctaattattg
    27061 aactaattaa taccacacta actgtttcag ttgatactaa gtgttaaaaa tgtgaatttt
    27121 aataacaaaa aagtataaaa aacacggtta tttattatta attatagtat tttcaattag
    27181 ataatgaatt ttgcataatg tgcttttttt accgtatgta ttcattttgt gaatgatgtg
    27241 tcgcggtaaa acgcactatt cgttaaatgt atcgcaggag aaagaagggg aatgagaggg
    27301 ataagcaaca ttttccccgc cgccagaagc gacggggcag agattaaagc tcctggtcga
    27361 acagctctaa aatcgcttcg tacaggtctt tgacggtgaa accgttagca ggggtggtaa
    27421 agatggtgtc atcgccagcg atggtgccca gaataccttc tgctttgccc agtgagtcca
    27481 gcaggcgagc aattaactgc gccgcgccag ggctggtatg aatcacgaca actgcatcgt
    27541 tgtagtcgat atccagcacc agattcttca atggactgga ggtggttggt acacccagtt
    27601 cagctggcag gcagtaaacc atttccattt tggcattgcg tgtacgtaca gcaccaaact
    27661 tggtcaacat ccgcgagact ttagactgat taatattgtc aaagccttgc tcctgcaacg
    27721 cggcgacgat ttcgccctgg gagctaaatt tctcttcttt aagtaatgct ttaaatgctt
    27781 taactagttc ttcttgctta gccgagcttc gcataagtca cccgatatgg tggttgatac
    27841 aacattattg tgcatacaga tgaattttta tgcaaacagt cagccctgaa gaaggctgaa
    27901 ataatgttat gaaagagcgg gattttatca aatttcgtta ttgagaaaca tgcctgcgtc
    27961 acggcatgca aattctgttt aaaagtaaat taattgttat caaattgatg ttgttttggc
    28021 tgaacggtag ggtatattgt caccacctgt tggaatgttg cgctaatgca taagcgactg
    28081 ttaattacgt aagttaggtt cctgattacg gcaattaaat gcataaacgc taaacttgcg
    28141 tgactacaca ttcttgagat gtggtcattg taaacggcaa ttttgtggat taaggtcgcg
    28201 gcagcggagc aacatatctt agtttatcaa tataataagg agtttaggat gaaagtcgca
    28261 gtcctcggcg ctgctggcgg tattggccag gcgcttgcac tactgttaaa aacccaactg
    28321 ccttcaggtt cagaactctc tctgtatgat atcgctccag tgactcccgg tgtggccgtc
    28381 gatctgagcc atatccctac tgctgtgaaa atcaaaggtt tttctggtga agatgcgact
    28441 ccggcgctgg aaggcgcaga tgtcgttctt atctctgcag gcgtagcgcg taaaccgggt
    28501 atggatcgtt ccgacctgtt taacgttaac gccggcatcg tgaaaaacct ggtacagcaa
    28561 gttgcgaaaa actgcccgaa agcgtgcatt ggtattatca ctaacccggt taacaccaca
    28621 gttgcaattg ctgctgaagt gctgaaaaaa gccggtgttt atgacaaaaa caaactgttc
    28681 ggcgttacca cgctggatat cattcgttcc aacacctttg ttgcggaact gaaaggcaaa
    28741 cagccaggcg aagttgaagt gccggttatt ggcggtcact ctggtgttac cattctgccg
    28801 ctgctgtcac aggttcctgg cgttagtttt accgagcagg aagtggctga tctgaccaaa
    28861 cgtatccaga acgcgggtac tgaggtggtt gaagcgaaag ccggtggcgg gtctgcaacc
    28921 ctgtctatgg gccaggcagc tgcacgtttt ggtctgtctc tggttcgtgc actgcagggc
    28981 gaacaaggcg ttgtcgaatg tgcctacgtt gaaggcgacg gtcagtacgc ccgtttcttc
    29041 tctcaaccgc tgctgctggg taaaaacggc gtggaagagc gtaaatctat cggtaccctg
    29101 agcgcatttg aacagaacgc gctggaaggt atgctggata cgctcaagaa agatatcgcc
    29161 ctaggcaaag agttcgttaa taagtaattg attagcgaat aataaaaaac cggagcacag
    29221 actccggttt ttgttttgag cactcgactt aattggttgc cggatattcc tgaatggtga
    29281 cctgcagcgt taactgctta tcatcacgca tcactacaac cgggatcacc gaaccagggc
    29341 gaatttccgc cacctgatcc atcgtctcca gagcagagat ggccggtttg ttatccaccg
    29401 aaataatcag atcgttgacc tgaatacccg cattcgccgc tgggccgtca ggtgacactt
    29461 cattaaccac gatcccttgc agttgatcta taccaccgct ctgcgcgtgc agtggtgcga
    29521 tctcgcgtcc gccgatacca atgtagccgc ggatcacgcg accatcgcgg atcagcttat
    29581 ccataatttt ggttgctaac tggaaaggaa tcgcaaagcc gataccttcc ggcgtttcgc
    29641 catcgttact cttatcaaac gacagcgtgt taatgcccat cagttctccc agcgagttca
    29701 ccagcgcgcc gccagagtta ccgtggttaa tggaagcatc ggtttgtagg aagttttgcc
    29761 gcccggtcgg gttcagaccg attcgacccg tggcactaat aatcccttgg gtaatggtct
    29821 gcccgaggtt gtacgggtta ccgatcgcca gtactacgtc gccaatgtgc ggtacgcgac
    29881 gtgcattaat tggaatggta ggtaaaccgc cagtggcatt aattttaagt accgccagat
    29941 cggttagaga gtcagatccc accagcaatg cttcaaatac acgtccatcc tgtaaggcga
    30001 cgatgatctg atcggcgtcg ttgatgacgt gtttattggt gatgatataa ccgcgttgat
    30061 ccatgattac accggatccc agggtgcgga tctcaagctg gttgtgagag ttggtgttca
    30121 aaccacggtt gtaaacgtta accaccgctg gcgcggcgcg gcgaaccgcc agattatagc
    30181 tggcaggcgt ctcatcggta ctgtcaaatt gcggagtgga aagcgggtta aggctgcgca
    30241 gcgaaggcat ggcaaccagc agaatagcgc cgacaattaa tccaatcgca acggaacgta
    30301 agagcttcac aaacatgatg gaggcgtcat taaaaaaggg aacggcagca gcataccacg
    30361 agttaaccgg acatcacacg tcagcctgat gcccggttta cgacattaac gcatcagcag
    30421 atagatgctt tcattgccgc gtacaatttg cagggcgatg atggccggtt ttgccgccag
    30481 cactttacgc atttcagcaa tcgagttcac ccgatcgcgg ttgacgccaa tgatcacatc
    30541 gtctttttgc aagccagcct gagcagctgg gcttcctttg acaacttcat ctatcttaat
    30601 acctttgccg ccatctttta gctgaccatc gctcaacgtt gcaccttcca gcgctggccg
    30661 acgaagaggt gctggtatca agcgtcactt ctacttccag tggtttgccg ttacgcagca
    30721 ggccaagctt cactttcgtg cccggctctg tagtcgcgat acgagagcgc aactcagcaa
    30781 agctattcag cggtttgccg ttgaggctgg taataatatc gcccgctttg acgcccgctt
    30841 tcgctgagcc agaacttggc aacacttcgc tgacaaacgc gccacgctgc acgtcaaggt
    30901 tgaaggcttt ggcgatatcg gcactcatct cggtgccttt gatgcctaac aaaccgcgtt
    30961 tgatttcacc aaagtcgata agctgctgcg ccagtgttcg cgccatatta ctggggatgg
    31021 caaatccaat cccgacgctc ccgccgccag gcgcaaggat tgcagtgttg atgccaatta
    31081 actcaccgtt aaggtttaat agtgcaccgc cggagttacc gcggttaatg gaagcatctg
    31141 tctggataaa gttttccaga ccttcaagat tcaacccgct gcggcctaat gcggaaacaa
    31201 tgccagaggt ggcggtttgc ccaaggccaa atgggttacc gaccgctacg gcaaaatcac
    31261 cgacgcgcaa tttatcggag tcggcaatag cgatttgcgt taatttgctc gggttttgaa
    31321 tttgtaacag ggcgatatcg ctctggtcat cgctaccaat cagttttgca tcaaactcgc
    31381 gcccatcatt gagctgaata ctgattttct gtgcctgatt aatcacatgg ttgttggtca
    31441 gcacatagcc tttactggcg ttgatgatga caccggaacc taaaccttcg aagggttgtg
    31501 caggttgatc cggtaaatca tcaccaaaaa actttttgaa ttcttccggg attttctgtc
    31561 cctgactggc cgttccttcc acccgtacgc tcaccactgc cggaagcact ttttccagca
    31621 ttggagccag actggggaga ggggcctgat cggcaacctg gcctggaatc gacgcgacgg
    31681 cctgaaatga cgccgagaga gttaacccgg cacttaacgc taatgcactc aacagctggg
    31741 tttgtttttt cattattcct gctctcgtac ctgaatgata agaaaagaga ttcaaaacag
    31801 tttgatgtta ttgaattttc agacggctaa caatgagaca ccagattaaa tgatagtcgg
    31861 ggagggagag aagaggaggg cgcaatggct gcgcccgaaa aataaattag tcgcgcttcg
    31921 cgccagtacg cagcaggccg gatgcgcctt cagaatagtc gcgaggcacc tgcaccggtg
    31981 cctggtcgtt gctggcttca gactctgcca gacgattacg gaacgggttt gcttcagcag
    32041 acagttccgg cagcaggctg ctggagcttt ttgccatgtg ctgatacagc tggcgatagt
    32101 cgtgcgccat ggtatccagt aattccgcgc tgcgggcaaa gtggctaacc agctcttcgc
    32161 gatactcgtc cagttcagct ttattctttt ccagttcgta ctgcaacgcc tgttgctggc
    32221 gtagtttacg attaccaaaa cgcatggcca cagcaccaat aatgatgccg acgactaacc
    32281 caattagcgc atattcccag gtcatgaaca tctcccgttg tcttttgttt ccgtagggtg
    32341 ttggcttcag gctccatgcc tgcggctgat tatgccacta taaccgttaa ttccacagaa
    32401 gtggaatccc gactgcatat cgcgtagtgt agaacggcct ttttttcgtc aaccgtgaac
    32461 aacagcgcac cgattattca aggaataaca ataagatcat gcaaagcgtt accccaacat
    32521 cgcaatacct gaaggcgctc aatgaaggca gccatcaacc cgacgacgtt caaaaagagg
    32581 ccgtcagccg cctggaaatt atttatcaag aactcatcaa tagcaggcca ccagccccca
    32641 ggacgagtgg gctaatggcg cgggtcggta agctgtgggg taaacgcgaa gacacaaagc
    32701 atacgccagt gcgtggctta tatatgtggg gcggtgtagg acgcgggaaa acctggctga
    32761 tggacctttt ctatcaaagc ctgccgggag agcggaaaca gcgcctgcac tttcaccgtt
    32821 ttatgctgcg ggtgcacgaa gagctaactg ccttacaggg gcagaccgat ccgctggaaa
    32881 ttattgccga tcgctttaaa gccgaaactg acgtgctctg ttttgacgaa ttttttgttt
    32941 ctgatattac cgacgccatg ctacttggcg gtctgatgaa agccctgttc acccgcggta
    33001 ttaccctggt agcgacgtca aatattccgc cggacgaact ttatcgaaat ggcctgcaac
    33061 gtgcgcgttt tctgcctgca atcgatgcca ttaaacagca ttgtgatgta atgaacgtgg
    33121 acgctggtgt tgattatcga ctgcgtacac tcactcaggc gcatctgtgg ctttcgccac
    33181 tcaacgatga aacccgggcg cagatggata aactatggtt ggcgctggcg ggggtgaaac
    33241 gagaaaattc accgacgtta gaaatcaacc atcggccatt ggcgacaatg ggcgtcgaga
    33301 accagacgct ggcggtttct tttactacgc tgtgcgtcga cgcccgcagt cagcatgact
    33361 atattgcgct ctcacgcctg tttcacacag taatgttgtt tgatgtgcca gtcatgacgc
    33421 ggttgatgga gagcgaagcg cggcgcttta ttgcgctggt ggatgagttt tacgagcgcc
    33481 atgtcaaatt agtggtgagt gcagaagtgc cgctgtatga aatttatcag ggtgagcggc
    33541 tgaagtttga gttccagcgt tgcctgtcac gtctgcaaga gatgcaaagc gaagagtatc
    33601 tgaagcgcga gcatttggcg ggttaaaacc tgccacaaat cacaaaaagg ggtcgatctt
    33661 tgaccccgac ttctctataa tcctgcgacc ccacgttaca agaaagtttt tttccccaaa
    33721 actttttgtg tgctggcata ggctattcga aggggtaggt ttgccggact ttgtcgtgtg
    33781 aacctcaaca attgaagacg tttgggtgtt caccaacgtg taactattta ttgggtaagc
    33841 ttttaatgaa aacttttaca gctaaaccag aaaccgtaaa acgcgactgg tatgttgttg
    33901 acgcgaccgg taaaactctg ggccgtctgg ctactgaact ggctcgtcgc ctgcgcggta
    33961 agcacaaagc ggaatatact ccgcacgtag ataccggtga ttacatcatc gttctgaacg
    34021 ctgacaaagt tgctgtaacc ggcaacaagc gtactgacaa agtgtactat caccacaccg
    34081 gccacatcgg tggtatcaaa caagcgacct ttgaagagat gattgctcgc cgtcctgagc
    34141 gtgtgattga aatcgcggtt aaaggcatgt tgccaaaagg cccgctgggt cgtgctatgt
    34201 tccgtaaact gaaagtttac gcgggtaacg agcacaacca cgcggcacag caaccgcaag
    34261 ttcttgacat ctaatcggga ttataggcaa tggctgaaaa tcaatactac ggcactggtc
    34321 gccgcaaaag ttccgcagct cgcgttttca tcaaaccggg caacggtaaa atcgtaatca
    34381 accaacgttc tctggaacag tacttcggtc gtgaaactgc ccgcatggta gttcgtcagc
    34441 cgctggaact ggtcgacatg gttgagaaac tggacctgta catcaccgtt aaaggtggtg
    34501 gtatctctgg tcaggctggt gcgatccgtc acggtatcac ccgcgctctg atggaatacg
    34561 acgagtccct gcgttctgaa ctgcgtaaag ctggcttcgt tactcgtgac gctcgtcagg
    34621 ttgaacgtaa gaaagtcggt ctgcgtaaag cacgtcgtcg tccgcagttc tccaaacgtt
    34681 aattggcttc tgctacggca gaaaacaatt tgcgaaaaaa cccgcttcgg cgggtttttt
    34741 tatggctaaa ttctgaatca gcgtaaaaaa tgggaagttg ttttttgctg ccgtctgaaa
    34801 gacaggtaaa acaaaaacca catcgccaat aagggtctaa gtcaactatt tcagactaaa
    34861 gcgcatctct ttttccccat ttccggcatc cactcaccac aaaggtcgca aaatctggta
    34921 aactatcatc caattttctg cccaaatgtc gggtattgct cattttttgt ttgattttcg
    34981 aacaaagaga gtcgttcctt attgggtaac acaacttctg actggccacc tggtggctgg
    35041 tagcagtaaa aattctgact atacctggag gttttcatgg ctgtcgctgc caacaaacgt
    35101 tcggtaatga cgctgttttc cggtcctact gacatctata gccatcaggt ccgcattgtg
    35161 ctggctgaga aaggtgtaag tttcgagatc gaacacgtgg aaaaggacaa tccgcctcag
    35221 gatctgattg acctcaaccc gaatcagagc gttccgaccc tggtggatcg tgagctgacc
    35281 ctgtgggaat ctcgcatcat tatggaatat ctggatgagc gtttcccgca tccgccactg
    35341 atgcctgttt acccggtagc tcgcggtgaa agccgtctgt acatgcatcg catcgaaaaa
    35401 gactggtaca cgctgatgaa cactatcatc aacggttcag cttctgaagc agatgccgca
    35461 cgtaagcaac tgcgcgaaga actgctggcg attgcgccgg tcttcggtca gaagccgtac
    35521 ttcctgagcg atgagttcag cctggtcgat tgctaccttg ctccgctgct gtggcgtctg
    35581 ccgcaactgg gcatcgagtt cagcggcccg ggtgcgaaag agctgaaagg ctatatgacc
    35641 cgcgtctttg agcgtgactc tttccttgct tctttaactg aagcagaacg tgaaatgcgt
    35701 ctgggccgga gttaatctgt atggatttgt cacagctaac accacgtcgt ccctatctgc
    35761 tgcgtgcatt ctatgagtgg ttgctggata accagctcac gccgcacctg gtggtggatg
    35821 tgacgctccc tggcgtgcag gttcctatgg aatatgcgcg tgacgggcaa atcgtactca
    35881 acattgcgcc gcgtgctgtc ggcaatctgg aactggcgaa tgatgaggtg cgctttaacg
    35941 cgcgctttgg tggcattccg cgtcaggttt ctgtgccgct ggctgccgtg ctggctatct
    36001 acgcccgtga aaatggcgca ggcacgatgt ttgagcctga agctgcctac gatgaagata
    36061 ccagcatcat gaatgatgaa gaggcatcgg cagacaacga aaccgttatg tcggttattg
    36121 atggcgacaa gccagatcac gatgatgaca ctcatcctga cgatgaacct ccgcagccac
    36181 cacgcggtgg tcgaccggca ttacgcgttg tgaagtaata caaaacaggc ccaggcggcc
    36241 tgttttgtct ttttaatgac ttaataaaat agcgtgattg tggcaatcat attcaccacg
    36301 aaaccgactg ccatggggat cgctgtccgc ttcaccaccg caaaaggcga aacgcctgca
    36361 attcctgacg taacgaccac caccgcagta atcggcgaaa ccgcgcgcgc tagcgtcgtg
    36421 gcaaaatgca tcggcataat cattacaacc gctggtacat gtagtccggc tgcgatattc
    36481 ggaataagac tggcaaatga cataaacggc gcattgccag agcccatcac aatggcacaa
    36541 atggcaatga ctagcgccat aataatcatc acgccaatac cgcccagacc agaatgctcc
    36601 gcacccctga taaccgcatc aacagtgcca atcgtcgtta agcctttcgc aaatatttca
    36661 cccgcgacca ccagcgttac cacgttgaca aactgcgtac ccatgccgtc aaaaaacgcc
    36721 tgcacatcgt ccattgtttc gcgcaagtta tgcttgcgga agaactcaac aaacatcgtc
    36781 accgtcaaac ttagtaacat caccaccacc agatgcagtt ctgacttcat cagcccgacg
    36841 tgggcgagga acagcgagcc gagcatcagg attaacggca tcacaggtaa aatggcgtaa
    36901 tagagcggcg ggacattatc gagagttttt tgctctgcct gttcgtgatt gatattttta
    36961 tcttttttgt caaaagcgcg ttgcacgaaa aagtgggaga tcgccaccga gataatgacg
    37021 caagaggcga ccggaagctg gtagtggaag aagtatgtgg caattttcat tcccgcgacc
    37081 tgggctgcaa aaatggagtt cgtttccaga atcccccact caatggacat cgtggttgcg
    37141 ataaccgcca ccgcagagag gcgacttact cccagactca ccagcgtcgg aaataaggtg
    37201 accatcagca acatacccag accggaggcg ctggtaataa actgcgccat gatttggccg
    37261 atgacgtaag ttgccgacag aataatatac ggcgagcgaa tgagttttaa cgggcggctt
    37321 agcaagctca ccatcgcgca actggctcct atgcgctcca tgtagcgggc ataaccgccc
    37381 accgccataa tcgacagccc caatccggca atgcggttgg acagcatgcg caggatttca
    37441 ttataaatgt cgacgatcaa gtagccgctg cttttggcgg gatcgagtac accaccgaac
    37501 cctaaccaga ctccgcacat catcaggaag acacctgcgg cagccagcac cacctgaggt
    37561 ttgtagtttt tcaggatcaa atagcccatc gtaattaata cgatgacaga tataattatg
    37621 ccgaacatt
//
LOCUS       DAJTQY010000029        36833 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.029, whole genome shotgun sequence.
ACCESSION   DAJTQY010000029 DAJTQY010000000
VERSION     DAJTQY010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36833
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.029"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            <1..247
                     /locus_tag="OO349_001563"
     CDS             <1..247
                     /locus_tag="OO349_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HCR5866687.1"
                     /translation="VTIPRGQLKNLKASYTLTEPQLTAPLKKGQVVGTIDFQLNGKSI
                     EQRPLIVMENVEEGGFFGRMWDFVMMKFHQWFGSWFS"
     gene            complement(294..1052)
                     /gene="deoR"
                     /locus_tag="OO349_001564"
     CDS             complement(294..1052)
                     /gene="deoR"
                     /locus_tag="OO349_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HCR5866688.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(1110..1706)
                     /gene="ybjG"
                     /locus_tag="OO349_001565"
     CDS             complement(1110..1706)
                     /gene="ybjG"
                     /locus_tag="OO349_001565"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCR5866689.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            1991..3217
                     /gene="mdfA"
                     /locus_tag="OO349_001566"
     CDS             1991..3217
                     /gene="mdfA"
                     /locus_tag="OO349_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HCR5866690.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTLLRFLQGISFCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETAMRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLARL
                     TSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGLVR
                     LTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWLSL
                     MVIFLKDKQMGNSHEG"
     gene            complement(3258..3542)
                     /locus_tag="OO349_001567"
     CDS             complement(3258..3542)
                     /locus_tag="OO349_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5866691.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(3628..4443)
                     /gene="ybjI"
                     /locus_tag="OO349_001568"
     CDS             complement(3628..4443)
                     /gene="ybjI"
                     /locus_tag="OO349_001568"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HCR5866692.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVMSEGKDVFNGELSKDAFATVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGYNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(4443..5651)
                     /locus_tag="OO349_001569"
     CDS             complement(4443..5651)
                     /locus_tag="OO349_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5866693.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     SYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            5735..6271
                     /gene="rcdA"
                     /locus_tag="OO349_001570"
     CDS             5735..6271
                     /gene="rcdA"
                     /locus_tag="OO349_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HCR5866694.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(6446..8131)
                     /locus_tag="OO349_001571"
     CDS             complement(6446..8131)
                     /locus_tag="OO349_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HCR5866695.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLHHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            8401..8778
                     /locus_tag="OO349_001572"
     CDS             8401..8778
                     /locus_tag="OO349_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HCR5866696.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(8808..9065)
                     /gene="grxA"
                     /locus_tag="OO349_001573"
     CDS             complement(8808..9065)
                     /gene="grxA"
                     /locus_tag="OO349_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HCR5866697.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            9225..9512
                     /locus_tag="OO349_001574"
     CDS             9225..9512
                     /locus_tag="OO349_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HCR5866698.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            9496..10218
                     /gene="nfsA"
                     /locus_tag="OO349_001575"
                     /pseudo
     CDS             9496..10218
                     /gene="nfsA"
                     /locus_tag="OO349_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
     gene            10279..11181
                     /gene="rimK"
                     /locus_tag="OO349_001576"
     CDS             10279..11181
                     /gene="rimK"
                     /locus_tag="OO349_001576"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HCR5866699.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGYFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            11269..11745
                     /locus_tag="OO349_001577"
     CDS             11269..11745
                     /locus_tag="OO349_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HCR5866700.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            12097..13209
                     /gene="potF"
                     /locus_tag="OO349_001578"
     CDS             12097..13209
                     /gene="potF"
                     /locus_tag="OO349_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HCR5866701.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYVNANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            13304..14437
                     /gene="potG"
                     /locus_tag="OO349_001579"
     CDS             13304..14437
                     /gene="potG"
                     /locus_tag="OO349_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HCR5866702.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEISSRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            14447..15400
                     /gene="potH"
                     /locus_tag="OO349_001580"
     CDS             14447..15400
                     /gene="potH"
                     /locus_tag="OO349_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HCR5866703.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDSLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            15397..16242
                     /gene="potI"
                     /locus_tag="OO349_001581"
     CDS             15397..16242
                     /gene="potI"
                     /locus_tag="OO349_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HCR5866704.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGSSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            16302..16790
                     /locus_tag="OO349_001582"
     CDS             16302..16790
                     /locus_tag="OO349_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HCR5866705.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            16831..17958
                     /gene="rlmC"
                     /locus_tag="OO349_001583"
     CDS             16831..17958
                     /gene="rlmC"
                     /locus_tag="OO349_001583"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HCR5866706.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKTLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLHE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(18133..18864)
                     /gene="artJ"
                     /locus_tag="OO349_001584"
     CDS             complement(18133..18864)
                     /gene="artJ"
                     /locus_tag="OO349_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein ArtJ"
                     /protein_id="HCR5866707.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVISKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(19156..19824)
                     /gene="artM"
                     /locus_tag="OO349_001585"
     CDS             complement(19156..19824)
                     /gene="artM"
                     /locus_tag="OO349_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HCR5866708.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(19824..20540)
                     /gene="artQ"
                     /locus_tag="OO349_001586"
     CDS             complement(19824..20540)
                     /gene="artQ"
                     /locus_tag="OO349_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HCR5866709.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMCRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(20547..21278)
                     /gene="artJ"
                     /locus_tag="OO349_001587"
     CDS             complement(20547..21278)
                     /gene="artJ"
                     /locus_tag="OO349_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5866710.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEAPYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(21296..22024)
                     /gene="artP"
                     /locus_tag="OO349_001588"
     CDS             complement(21296..22024)
                     /gene="artP"
                     /locus_tag="OO349_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HCR5866711.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(22242..22757)
                     /locus_tag="OO349_001589"
     CDS             complement(22242..22757)
                     /locus_tag="OO349_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5866712.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATQLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            23406..25175
                     /locus_tag="OO349_001590"
     CDS             23406..25175
                     /locus_tag="OO349_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCR5866713.1"
                     /translation="MINNISDQASSFPGTQLNQSDNFLDSLREFFAILNSSRKGELST
                     WDTIYLHLILAINADSDLIKNDVLLAENIPSANYQFNTFFSNTFEIDVKKYLGKSEDN
                     EVEIKAGNERISIGIRNVSNGKLERQQFLFPLDYENKLQEQLDKYFTIESHPLLYRYT
                     IGSKIANVIFEKLYSRIDFNKEQYISFIKDAFIHFYDYSRRYAISENIDKDAVTNNIA
                     LMSTFYDSDNTSGEVLNNDFTEEESFETALDVEHAIVLGFADDNFETKPVHYQDLLTR
                     FSAFQDTVFNLFPEMHSSHYHDICSVSVDMTKGTQCMIHLMVNEEVFMSLPVPVATMV
                     REDASNLVNLKTLLNDGCFIKYSHFNDVALIKQNISNLYLSHTVVNESILKKCCFENG
                     SLGDVKITNSNVINSAFKNISFRSVKINNVNTHSLKFINCNFFNVDMIRVNLSKCLFH
                     ECSMHGVKIKPWLPVKWTKELISDYLYGCLLSLYSICARDIYNMNAGNNVKVAADAFL
                     EIIFSLKNKYCIKLLSAQDRAFIYEFARMIFAYINDKSIEILLLSCFAAADQKAIQRY
                     RPQSQDGEDFRSHLQYKLPLSAH"
     gene            25386..25709
                     /locus_tag="OO349_001591"
     CDS             25386..25709
                     /locus_tag="OO349_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HCR5866714.1"
                     /translation="MQFSTTPTLEGLTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            25706..26536
                     /gene="amiD"
                     /locus_tag="OO349_001592"
     CDS             25706..26536
                     /gene="amiD"
                     /locus_tag="OO349_001592"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HCR5866715.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRHQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLIPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSSGVKYFAPFEQAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(26533..27546)
                     /locus_tag="OO349_001593"
     CDS             complement(26533..27546)
                     /locus_tag="OO349_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCR5866716.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRASGRNEAMGKLLEKMG
                     AEFVPTDLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRNIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(27645..29075)
                     /locus_tag="OO349_001594"
     CDS             complement(27645..29075)
                     /locus_tag="OO349_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HCR5866717.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQLALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPANEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWLVVNQIGGKERYFFGNILWQTRALMDRAIGHNLAKGRPEREYLQTGDAVD
                     SWKVIVVEPKKQLTLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(29086..30087)
                     /gene="ltaE"
                     /locus_tag="OO349_001595"
     CDS             complement(29086..30087)
                     /gene="ltaE"
                     /locus_tag="OO349_001595"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HCR5866718.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(30124..31842)
                     /gene="poxB"
                     /locus_tag="OO349_001596"
     CDS             complement(30124..31842)
                     /gene="poxB"
                     /locus_tag="OO349_001596"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HCR5866719.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNNVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVMPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKTDRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(31975..32943)
                     /gene="hcr"
                     /locus_tag="OO349_001597"
     CDS             complement(31975..32943)
                     /gene="hcr"
                     /locus_tag="OO349_001597"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HCR5866720.1"
                     /translation="MTMPTNQCPWRIQVHHITQETPDVWTISLICHDHYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLADVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(32955..34607)
                     /gene="hcp"
                     /locus_tag="OO349_001598"
                     /gene_synonym="priS"
     CDS             complement(32955..34607)
                     /gene="hcp"
                     /locus_tag="OO349_001598"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HCR5866721.1"
                     /translation="MFCVQCEQTIRTPAENGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLNA"
     gene            complement(34751..35650)
                     /gene="lysO"
                     /locus_tag="OO349_001599"
     CDS             complement(34751..35650)
                     /gene="lysO"
                     /locus_tag="OO349_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HCR5866722.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKAINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            complement(36107..36802)
                     /gene="aqpZ"
                     /locus_tag="OO349_001600"
     CDS             complement(36107..36802)
                     /gene="aqpZ"
                     /locus_tag="OO349_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HCR5866723.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
BASE COUNT         9214 a         9153 c         9442 g         9024 t
ORIGIN      
        1 cgtgaccata ccgcgtgggc agctgaaaaa cctgaaggcg agctacacgt taacggaacc
       61 gcagcttacc gcaccgctga aaaaaggtca ggttgtcggg accattgatt tccagcttaa
      121 cggtaaatcc attgagcagc gtccgctgat cgtgatggaa aatgtggaag agggcggatt
      181 ctttggtagg atgtgggatt tcgtgatgat gaaattccat cagtggttcg gcagctggtt
      241 ctcttaatct tctgataacc ggatggcgcg aaacgtcatc cggttatacg tcattaatac
      301 atcaacttaa tacggtgcgt ctgcgcgtac ttcacatact catcttccgg gcaacaatca
      361 ctcaccacaa tatcaaagcg tttcaggtca cccatgcgcg ccggacgcac cttgccaaat
      421 ttactgtggt cgacaaccag cacatgcttt tgcgccatcg acatggccca gtgttttacc
      481 ggcaactctt caagattaaa acaggtagcg cctttactga catgcacgcc cgccgcggaa
      541 taaaaagcga tatccgggca aaaattattc aacgtttgct gaaaatcgat gggtttgaaa
      601 atggcgttgc tggcgtgaaa ttcaccaccg cacagaaatg cgcggcaatg gggtttctct
      661 ttcagcgcca gaaaggtatt tagcgaataa caaacggcgg taaaagggat ttcattatca
      721 atcgcttcaa taatccacgg cgtggtggtg ccacagtcaa aaaagagggt ctgatcgggt
      781 tctaccagtg tcgcagccag ttttgccgcc cggcgttttt cttccaccag gcgggatttt
      841 tgatcgctta acaggtaatg gctggcactg cgcggttcca gaacaatata gccgccgagc
      901 aaaacgacgg gcgcactgtg gttgttcaga tcgcgacgaa tcgtcatctc cgaaacccca
      961 agcagggcgg cggcgtcttt aagatgtaac ttatcgctgc gttttaattc ttgcagcagc
     1021 tgcccgatac gctcttcgcg acgtgtttcc ataatccctc tgaatagtta ttgaagcgag
     1081 ccgctcaata ctacactttt tagcagagat cagtcacgca cccagccttt gcggatcggt
     1141 aatgcaaaac agacgcgata ccacgattgc agacgttgat agagtttatg ccccatcgct
     1201 tgccagataa tctgggcact aaggcagcca atcatacctg ccagcaatcc accgagcata
     1261 tccagcggcc agtggacgcc aagataaacg cgcgaccagg caatgacgac ggccagcacc
     1321 attaaaagtg agccggacca caggcgatgc cagcataaaa atgccagtgc aaaggtgaaa
     1381 atcaccgtac cgtgatcgct cgggaatgag tcatccgccg catgatgcag gaagttatag
     1441 ccgatatttt cgacaaaggg tcggtcgtgc ggaaaaagat gtcccatcgt ccaggacaca
     1501 aacaggctga ccgccagcgc gatagcgatt tttatcacca gttgccgttg tgctgtaagc
     1561 ccccacaacc aaagtaccgc ggccagcaac ggcaccacgg taatcaaatc tttagcaata
     1621 aaaatcgcca acgagatcat ccacggagcc gagtctggcg tcgcgttaat aagagagaat
     1681 agagagagat tcaaattttc cagcataact tcccgacgca aagtgattaa aagggagcca
     1741 atacaggcaa gtcgttgaga ataaagtgca ggttaaactg ggtaaagcgg catcgtctta
     1801 tttccctcaa gcggcctgtt tacggtgggt gattgtaacg ggcataggtt aaataaaact
     1861 taaagaaagc gtagctatac tcgcaataat gtaagaatgt gcttaaccgt ggtttcagct
     1921 acaaaattcg ctttctcgtt agctgcgctt ttattaaact ctgcgcgatt attattggcg
     1981 aagaaattgc atgcaaaata aattagcttc cggtgccagg cttggacgtc aggcgttact
     2041 tttccctctc tgtctggtgc tttacgaatt ttcaacctat atcggcaacg atatgatcca
     2101 acccggtatg ttggccgtgg tggaacaata tcaggcgggc attgattggg ttcctacttc
     2161 gatgaccgcg tatctggcgg gcgggatgtt tttacaatgg ctcctggggc cgctgtcgga
     2221 tcgtattggt cgccgtccgg tgatgctggc gggtgtggtg tggtttatcg tcacctgtct
     2281 ggcaatatta ctggcgcaaa acattgaaca attcaccctg ttgcgcttct tgcagggcat
     2341 aagcttctgt ttcattggcg ctgtgggata cgccgcaatt caggaatcct tcgaagaggc
     2401 ggtttgtatc aagatcaccg cgctgatggc gaacgtggcg ctgattgctc cgctacttgg
     2461 tccgctggtg ggcgcggcgt ggatccatgt gctgccttgg gaggggatgt ttgttttgtt
     2521 tgccgcattg gcagcgatct cctttttcgg tctgcaacga gctatgcctg aaaccgccat
     2581 gcgtataggc gagaaactgt cactgaaaga actcggtcgt gactataagc tggtgctgaa
     2641 gaacggccgc tttgtggcgg gggcgctggg attcgttagc ctgccattgc tggcgtggat
     2701 cgcccagtcg ccgattatca tcattaccgg ggagcagttg agcagctatg aatatggctt
     2761 gctgcaagtg cctattttcg gggcgttaat tgcgggtaac ttgctgttag cgcgtctgac
     2821 ctcgcgccgc accgtacgtt cgctgattat tatgggcggc tggccgatta tgattggtct
     2881 gttggtcgct gctgcggcaa cggttatctc atcgcacgcg tatttatgga tgaccgccgg
     2941 gttaagtatt tatgctttcg gtattggtct ggcgaatgcg ggactggtgc gattaaccct
     3001 gtttgccagc gatatgagta aaggtacggt ttctgcggcg atgggaatgc tgcaaatgct
     3061 gatctttacc gtcggtattg aaatcagcaa acatgcctgg ctgaacgggg gcaacggact
     3121 gtttaatctc ttcaaccttg tcaacggaat tttgtggctg tcgctgatgg ttatcttttt
     3181 aaaagataaa cagatgggaa attctcacga agggtaaaaa aatgcctgat tgatttgtgc
     3241 gatcaggcat tctcgaatta atggtgatgg tcgtcaatct ggtgttcgat aaccatccct
     3301 tcacctacgc tggcaagatg gcgaacataa ggatgcgggc ggtaagccgg agctggcgca
     3361 ggagccacat agacggtttg cggcacagtc gcgacgctga ccgcttgtgg aacgctgact
     3421 gaatcaggaa ccacaaccac tttgtatcca ctcggcacat cgacggtgac actttgcgcc
     3481 atcgccacac cagtaaagcc cattaaaagc gcgcccaaca gtagacaatt tttcataatc
     3541 atttcgcctg aaataatgca gcgggttaat ttccgcgaat tatgcagcct tcattcaggc
     3601 attgtgttgc cgggcgtgct gccgttttta ttggtcaaat ggcgcttcgt gtttaagaac
     3661 tttatcgatc acatccagta cgccttcacg gttattgtag cctgcccggt attttgccgc
     3721 tgcgacgact gcgctgccgg cattttccat tgcaaaacta aagcctgcct gacgcagcat
     3781 ctcaatatcg ttaccgccat cgccaaagac caccacttcg ctgtcgtcta ttccccataa
     3841 tttctgcagt tggcgaaggc cattggcttt atgtacgccg gggataatca gatcgatgct
     3901 gccgttgccg gtgtggactg gcaccataat atcgccgatg gcctcatgta atgctttttg
     3961 tacttgtgga atcagttcat cggaaagatt caggccaaac ttaaagaaaa tatcttctaa
     4021 gttgtcaaag ttatcgacgt attccagacg gtgataatac atttccgcca ccgttttcat
     4081 ggcatcgtca tattttttga gtgtataggc gctatttttt ccgcaggcaa taatttccac
     4141 ttccgggcgc gtcagcaaat gttccacgac agtagcaaac gcatccttcg acagctcgcc
     4201 attaaaaaca tctttgcctt cactcattac ccagccgccg ttttcagcca caaaggcaat
     4261 ttcattcgca atttcaggga agaaagagat caactgataa tattgattcc cgctggcgac
     4321 cacaaagcga atgccttgtg ctttcatttg ctgatactga gccataaacc gctcacggtt
     4381 ataggttttt tgatcgctta agaaagtacc atccatgtct accgcaatta atttaatgct
     4441 catcaactat tctccatcgc cgtctgcgtt ttggtatcgg gtttggcgac ggctttcgcg
     4501 acaatagccg cgagaataac cagcgccagt acaaccagca ttgcactacg taatccataa
     4561 tgttcgccga gatagcccag cagcggcggc ccgacgagga aagccagata accggtcgtt
     4621 gctaccacac tgacgcgggt tggtgcatcg gggccggtat cgctggcggc agaaatggtc
     4681 agcgggaagc ccagcgaggc acccagtccc caaagtacaa cagacactcc tgcgacccag
     4741 gcgctatcga caaaaataat cagcccaata cccaacgccc ccattagtgc actggcccga
     4801 accacggcaa cgcgactgta actgtcgatg aaccaaccgc cggtgaagcg tccaacggtc
     4861 atccccaggg taaaaccggc ataaatcagt gagccggaag tggggctaaa accgtgacca
     4921 tcaaccatta ataagggtaa ccagtcgttg gcagaacctt cggcaaaggc catcgccagc
     4981 actacaacac caatcaacag caactggata tcgcgataaa aaggaatgcc tttttcgcca
     5041 tgctgggtgc catcggcagc atttttgccc gtaccgtcag ggattgcctg aatggcgata
     5101 taaataggcg cgatacctac cagcgccgcc aataaaatgt gcaccgttgc cggaacgcca
     5161 aaggccgtca gtgccatccc gacaccagcg cctgccagcg tgcccaggct ataaaaaccg
     5221 tgcatcatcg gcaaaaccgt tttattcatt tctcgctcaa cggcggcacc ttcaacgttt
     5281 atcgccactt ccgcagaacc aaaacttgcc ccaaagacgc cgagaccaac ggcaaagagc
     5341 aggggcgatg tcagccagag tgccagactt aatatcatca tcccgatcaa tgcgcaggac
     5401 atcgtgacca ggatgacatt acgtgtccca aagcgtttca ctaaccacgc cgagcagaga
     5461 ataccgctca tcgaaccgat cgacagacca aagagaacac cgcccatttc agcgatcgag
     5521 acagagagaa tatcgcggat agcaggcgta cgggttgccc aggacgccat taacaggcct
     5581 ggcaaaaaga agaacataaa cagcgcccag gttcggcgtt tcaatgcatt acgtgaagaa
     5641 tttacggtca tagatcacgt caaaataaga agagggaaga caacattagc aaggttgtgt
     5701 acatttgtac acaattgcag acagaggaaa tgacatgcgt cgcgctaacg atccgcaacg
     5761 gcgagaaaaa attatccagg ccacgctgga ggcggtgaaa ctttacggaa tacatgctgt
     5821 tacgcaccgc aaaattgcta cccttgccgg ggtaccgttg ggatcgatga cctactattt
     5881 ttcaggaatt gatgagttgt tgctggaggc gttcagcagt tttactgaga ttatgtcccg
     5941 gcaatatcag gcatttttta gcgatgttag tgatgctcaa ggcgcatgcc aggctatcac
     6001 cgatatgatc tacagctcac aggttgcaac gccggataat atggagctga tgtaccagct
     6061 ctacgcgctg gctagccgaa aaccgctatt aaaaacggta atgcaaaact ggatgcagcg
     6121 cagtcagcaa acgctcgaac aatggtttga acccggaacc gcccgcgcgc ttgatgcgtt
     6181 tattgagggg atgacgctgc attttgtcac cgaccgtaag ccgctatcgc gcgaggagat
     6241 tttgaggatg gttgagaggg ttgcagggta gtagataaat tttagataac aaaaaaccca
     6301 tcaaccttga accgaaatgg cggggttgat gggctccaca aaatggggac atcaaagaaa
     6361 agcagtggca ctaattaaga ctgatgccct gcggaaaagt tctgcggttg tgcaaaaaaa
     6421 tttcattttc agggcaactt cagttttatc ctaatcctgg ccataccatg acgatgattg
     6481 tccctgccag cgtcagcagg acgttggcga ttgcgtaggt gcccgcatag cccagcgcag
     6541 ggatgttact gcgagctgta tcactgatga tctccattgc cggcgcgcag gtgcgtgcgc
     6601 ccatcattgc gccgaacaac agtgcgcggt tcattcgcaa tacataagca ccgaacaaga
     6661 aacagataac cacgggcaca agactgacaa ttaatccggc aatcaacatc tgaccgccaa
     6721 tcgcgcccag gccgttatta ataccgctac cggcgctcag accaacgcct gccataaaca
     6781 ccatcaagcc gaactctttc accatgctta atgcaccttg cggaatgtaa ccgaaggtcg
     6841 ggtggttagc acgcataaag cccagcataa ttccggcgaa taacaacccg gcagcgttcc
     6901 ccatgccgaa actgaatgtg ctgaactgga aggtgatcat cccgatcatc agcccaataa
     6961 caaagaaggc gcaaaatgcc agcaggtcag tgacctggct gtgaatcgag ataaagccga
     7021 tgcgatcggc gatggttttt acgcgacggg catcaccgct gacttgtaaa acgtcacctt
     7081 tgttaagcac gacgttgtca tctatcggca tctcaatctg gctacgaatg acgcggttaa
     7141 ggaagcaacc gtgatcggtc aacttcagtt gtgcgagacg tttacctaca gcgttatggt
     7201 ttttaacgac cacttcttca gtgacgatac gcatgtcgag aaggtcacga tcgaaaactt
     7261 ctttaccgtt acggaagctg ggatcgagtc gggcatgggc gtcgggatag cctaccaacg
     7321 ctatttcatc gcccatttgt agcacggcat caccgtctgg atttgccaga atcccgttac
     7381 gtcgaatacg ttcaatgtag cagccggttt gtcgataaat acccagttca cgcagatttt
     7441 tgccgtcggt ccaggccacc agctccgggc cgacgcgata ggcgcggatc accggtaaat
     7501 aaaccttacg gttggcatca gtgtccaggc cacgttcgcg ggcgatttgc tgggcgctgg
     7561 tctgtaagtc ctgatgctgc aatttcggca agtaacgcgc accaacaatc aaactcacca
     7621 gaccgattaa ataggttaag gcatacccga ggctcagatt atccagtgcc agtgagagct
     7681 gcctgctttc catgccggaa tgatgcagtg tatcgccagc accgaccaga accggtgtcg
     7741 acgtcataga gcctgctaac ataccggccg tcaggccaat atcccagcca aacagcttac
     7801 ctaaccctaa ggcgatcacc agcgcactgc caaccatcac cagtgctaac attaggtaat
     7861 ttttcccatc acgaaaaaaa atggaaaaaa agttcggtcc ggcttcgacc ccgacgcaga
     7921 aaataaacag cataaagcca agattaagcg catcggtgtt aatgctgaaa tgttgttggc
     7981 ctaataacag cgatacgact aaaacgccaa tggaattacc cagttggatc gaaccaagtc
     8041 gtaactttcc gagacatagc ccaagcgcga ggaccacaaa taataacaga atgtaattcc
     8101 catttaacaa ttcggcgacg tttatattca cggaggctaa cttcttgttt actagtaagc
     8161 tgttgaaaga aatggtaatt tacgataatg tgttttacca gaattcaggg cgcagattca
     8221 ttcagcgcac ctaaacgata gtaaagtaac aatatatttt actagtgtaa tcacattagg
     8281 tatcaacggc tatatgaatt gcgttggcct atattagcat ggaatgcgaa gcggctttat
     8341 cttactgaac gccacactgg cgaaaaatgt gttcgataga cgcagtgtca ggaggaacga
     8401 gtgaaacata aacaacgttg ggcgggggca atctgctgtt ttgtcctctt cattgtggtg
     8461 tgcctttttc tggcgacgca catgaaaggc gcttttcggg ctgccgggca tcctgaaatc
     8521 ggcttgctgt ttttcattct tcctggagca gttgccagtt tcttttcaca gcgtagagaa
     8581 gtcctgaaac ctctatttgg cgcaatgctg gcggcaccct gttcaatgct cattatgcgg
     8641 ctgttttttt caccgacgcg ctcattctgg caagagctgg catggttact aagcgcggtg
     8701 ttctggtgtg cgctgggggc actgtgtttc ttatttatca gtagtttgtt taaaccacag
     8761 cacagaaaaa atcagtaaag ccctcaacgc gagggcttgt cagacgatca ggcgtccaga
     8821 ttttctttca cccatgcagc aaaatcggta tagccgccga tatgttgctg atcgacaaaa
     8881 atctgcggca cggtttctac gggtttacct gccttttgtt gtagatcttc tttagtgatc
     8941 ccttccgcac gaatatctac atactgatac tgaaaatcat cgcgttcatt gctcaatttc
     9001 tcagccagat cttttgcacg cacacagtaa gggcaacccg aacgaccaaa aataacggtt
     9061 tgcattattt ctctcctcat agatttatgc ctgtaatgat cacgctaaaa tgtattcgct
     9121 gaaagtaggt ttaacctgtt gcattaattg ctaaaagcta taactgttaa acacaataca
     9181 gtgaaaagtt ttagactgaa ggctcacttt gcagagggaa gcgtatgcgc gcgatcggta
     9241 aattgcctaa aggcgtgttg atactggaat ttatcggaat gatgctactg gcggtggcgc
     9301 tgctgtcggt aagcgactcc ctgtcgctgc ctgagccatt ttctcggcca gaagtgcaga
     9361 ttctgatgat ttttctcggt gttttgctca tgcttcccgc tgcggtggtg gttattcttc
     9421 aggtggcaaa acgtcttgcc ccacagctga tgaaccgtcc accgcaatat tcacgttcag
     9481 aaagagaaaa agataatgac gccaaccatt gaacttattt gtggccatcg ctccattcgc
     9541 catttcactg atgaacccat ttccgaagcg cagtgtgagg cgattattaa cagcgcccgt
     9601 gcgacgtcca gttccagttt tttgcagtgt agtagcatta ttcgcattac cgacaaagca
     9661 ttacgtgaag aactggtgac gctgaccggc gggcaaaaac acgtagcgca agcggcggag
     9721 ttctgggtgt tctgtgccga ctttaaccgc catttacaga tctgtccgga tgctcagctc
     9781 ggcctggcgg aacaactgtt gctcggtgtc gttgatacgg caatgatggc gcagaatgca
     9841 ttaaccgcag cggaatcgct gggattgggc ggggtatata tcggcggcct gcgcaataat
     9901 attgaagcgg tgacgaaact gctgaaatta ccgcagcatg ttctgccgct gtttgggctg
     9961 tgccttggct ggcctgcgga taatccggat ctcaagccgc gtttaccggc ctccattttg
    10021 gtgcatgaaa acagctatca accgctggat aaagacgcac tggcgtagta tgacgagcaa
    10081 ctggcggaat attacctcac ccgtggcagc aataatcgct gggatacctg gagcgatcat
    10141 atccgccgaa caatcattaa agaaagccgc ccatttattc tggattattt gcacaaacag
    10201 ggttgggcga cgcgctaaaa ccgctacgtc gatgtatgat acgcgggctt ttgaccaggt
    10261 ctgacagaga ggtgcagggt gaaaattgcc atattgtccc gggatggaac gctctattcg
    10321 tgtaagcggc tgcgtgaagc cgctatacag cgcggtcacc tggttgaaat tcttgatccg
    10381 ctttcttgct acatgaacat aaatcctgcg gcgtcttcta ttcactacaa aggccgcaag
    10441 ttaccccatt ttgacgcagt gatcccgcgt attggcaccg ccattacctt ttatgggacg
    10501 gcggcactgc gccagttcga gatgctgggg agctatccgc tcaatgagtc ggtcgccatt
    10561 gcccgggcgc gtgacaaatt gcgttccatg caactgctgg cgcgtcaggg catcgacctg
    10621 cctgtcacgg gcattgcgca ttcgccggat gataccagcg atttaatcga catggtcggt
    10681 ggtgcgccgc tggtggtcaa gttggttgaa ggcacgcagg gaattggcgt cgtgctggcg
    10741 gagactcgtc aggcggcgga aagcgtgatt gacgctttcc gcggtctgaa cgcgcatatt
    10801 ctggtgcagg aatatatcaa agaggcgcaa gggtgcgata tccgctgtct ggttgttggt
    10861 gatgaagtgg tcgctgcgat tgaacggcgg gcgaaagagg gctattttcg ttccaatttg
    10921 catcgtggcg gcgcggcaag cgtcgccagt atcacaccac aggagcgtga aatcgcgata
    10981 aaagccgcgc gaacgatggc gctggacgtt gctggtgtgg atattctgcg tgctaatcgc
    11041 gggccgttgg tgatggaagt gaatgcgtcg ccggggctgg aaggaataga aaaaaccacc
    11101 ggtatcgata tcgcgggtaa aatgatccgc tggatcgaac gccacgctac gacagaatat
    11161 tgcctgaaaa cgggtggtta gtcgcaatca cattactgat catggttttg cctgcgcttt
    11221 ttgcgtaagc tgtgccggtc tttttatcga aagaggttgt acaaaattat gacatcgctg
    11281 gtcgttcctg gtctggatac gctgcgtcaa tggctcgatg acctggggat gagttttttt
    11341 gaatgtgata actgtcaggc tctgcatctg ccccatatgc agaatttcga cggtgtcttt
    11401 gatgccaaaa tcgatctgat cgataacacg atcctgtttt ctgccatggc ggaagtccga
    11461 ccttcagccg tattgccgct ggcggcggat ttatctgcca tcaatgccag ttcgctgacc
    11521 gtgaaagcat ttcttgatat gcaggatgat aatctgccaa agctggtggt ttgccagtct
    11581 ttatccgtta tgcagggcgt aacctatgag cagtttgcat ggttcgtgcg tcagagcgaa
    11641 gagcagattt cgatggtcat tcttgaagct aatgcccatc aactgctgtt accgactgac
    11701 gatgaagggc aaaacaacgt taccgaaaac tattttctcc actgataatt cctttcgagc
    11761 acgcagtcgc tgctgcagtg actgcgcgct gcaaaattat ctgctgtttt taaccttttc
    11821 ttaaagatta tttcacttct cttgtgtcga tttggcttta tcacatagag caaatacgca
    11881 taaaaatttg ttaaataccg ttttttaatc cgagctatag tctcaaaccc tggctaaagt
    11941 tattcttgcg atgcttctat atagcgagca gtgctggccg ggagagagtt ctcttttctt
    12001 acaccgcgcc gataaaaaaa tatgcacgtt tattgcatat ctttcagtgt gacaactttt
    12061 gttcgtttgt taacgaactt tcagaaggaa agagatgtga ccgccttaaa taaaaaatgg
    12121 ctatcgggtc tggttgcggg tgctctgatg gccgtctctg tcggcacgct cgcggctgaa
    12181 caaaaaacac tccacattta taactggtct gattatatcg cccctgacac ggtggccaat
    12241 tttgaaaaag aaaccggtat taaagtcgtc tacgatgttt tcgactctaa cgaagtactg
    12301 gaaggcaaat taatggcggg tagcaccggc tttgacttgg tggttccatc tgccagcttt
    12361 ctagaacgcc agttgactgc gggagttttc cagccgctgg acaaaagcaa attgccggag
    12421 tggaagaatc tcgacccgga gctgctgaag ctggtcgcca aacacgatcc cgacaataaa
    12481 tttgctatgc cctatatgtg ggcgacgacc gggattggct ataacgttga taaagttaaa
    12541 gctgtgctgg gcgaaaacgc gcccgtcgat agctgggact tgatcctcaa acctgaaaat
    12601 ctggaaaaac tgaaaagctg cggtgtctct ttcctggatg cgccagaaga agtttttgct
    12661 accgtgttga attatctcgg caaagatccc aacagcacta aagcggatga ttacaccgga
    12721 ccggcaaccg atctgctgtt aaagctgcgc ccgaacattc gttatttcca ttcatctcaa
    12781 tacattaacg acctggcaaa cggcgatatt tgcgtcgcta tcggctgggc aggtgatgtc
    12841 tggcaggcgt caaaccgcgc gaaggaagcg aagaatggag tgaatgtctc gttctcgatt
    12901 ccaaaagaag gggcgatggc gttctttgat gtattcgcca tgcctgcgga tgccaaaaac
    12961 aaagacgaag cctatcagtt cctgaattac ctgctgcgcc cggatgtagt agcgcatatt
    13021 tccgaccatg tgttctatgt caacgccaat aaagcagcca cgccgctggt gagtgcggaa
    13081 gtccgtgaga acccgggtat ttatccgcct gcggatgttc gtgcgaagct gttcactctg
    13141 aaagtgcagg acccgaaaat cgaccgtgtg cgcacccgcg cgtggaccaa agtgaagagc
    13201 ggaaaataat ccgcagcctt agatgccggg cgggcgcacc acacccgccg gcaattcgca
    13261 ccatcatggt gcgcttgcac acattcaatg ccggagagca gccgtgaatg acgctatccc
    13321 tcgcccgcag gcgaaaaccc gtaaggcgct gacgccgcta ttagaaatcc gcaacctcac
    13381 caaatcctac gatggtcaac atgcggtgga tgatgtcagc ctgaccatct acaaaggtga
    13441 aatcttcgcg ctgctgggcg catccggctg tggcaagtcc acgctgctgc gtatgctggc
    13501 aggtttcgaa caaccttctg ccggacagat aatgcttgat ggcgtcgatt tgtcacaggt
    13561 tccgccttac ctgcgcccca tcaatatgat gtttcagtct tacgcgctgt ttccgcatat
    13621 gaccgtggaa cagaacatcg cttttggcct gaaacaggac aaactaccga aagcggaaat
    13681 ttccagtcgg gtcaatgaga tgctcggcct ggtgcacatg caggagttcg ccaaacgtaa
    13741 accgcatcag ctttctggtg gtcagcgaca gcgtgtggcc ctggcccgaa gccttgccaa
    13801 gcgcccgaaa ctattactgc tcgacgagcc gatgggcgcg ctggataaaa agctgcgtga
    13861 ccggatgcag cttgaagtgg tggatattct ggagcgcgtc ggtgtgactt gtgtgatggt
    13921 cacccacgat caggaagagg cgatgaccat ggcggggcgc atcgccatta tgaatcgtgg
    13981 gaaatttgtc cagattggcg aaccggaaga gatctacgag catccgacta cccgctatag
    14041 cgccgaattt attggctcgg taaacgtctt tgaaggcgta ctcaaagagc gtcaggaaga
    14101 tggcctggtg cttgattcgc cggggctggt gcatccactg aaagtcgacg cggatgcctc
    14161 ggtggtcgat aacgtgccgg tacatgtggc gctgcgcccg gaaaaaatca tgctttgcga
    14221 agagccgccc gccaatggtt gtaacttcgc ggtgggggag gtgatacaca ttgcctatct
    14281 cggcgatctt tcggtgtatc acgttcgtct gaaaagtggg cagatgatta gtgcccagct
    14341 acaaaacgcc catcgtcatc gtaaagggtt accgacctgg ggcgacgaag tgcgtttgtg
    14401 ctgggaagtg gacagctgtg tggtgctgac ggtttaagga gcaaagatga atacacttga
    14461 acctgctgcc cagtcgaaac cgccgggcgg atttaagctg tggttgtcgc agctgcaaat
    14521 gaagcatggg cgcaaactgg tcattgcgtt gccatatatc tggttgatct tgctgtttct
    14581 gctgccattt ctgattgtct ttaaaataag cctggcggag atggcgcgcg ctattccacc
    14641 ttataccgaa ctgatggagt gggctgacgg gcaactgtcc atcactctta atctcggtaa
    14701 ttttctgcaa ctgaccgacg attcgctcta tttcgatgct tatctccagt cgctacaggt
    14761 ggctgcgatt tcgacatttt gctgtttact gatcggctat ccgctggcgt gggcggtggc
    14821 gcacagtaag ccttcgaccc gtaatatttt attactactg gtgatcctgc cgtcgtggac
    14881 ctcgtttctg atccgcgttt atgcttggat gggaatatta aaaaacaacg gtgtgctgaa
    14941 taattttctg ctgtggctgg gggttatcga tcaaccgctg accattcttc ataccaatct
    15001 ggcagtttat attggcattg tttacgccta cgtgccgttt atggtgctgc cgatttatac
    15061 cgcgttgatt cgtattgatt attcgctggt ggaagcagcg ctggatctcg gtgcacgacc
    15121 gctgaaaacg ttctttactg tgatcgtgcc gctgactaaa ggtgggatta ttgccggatc
    15181 gatgctggtg tttatcccgg ctgtgggcga gtttgtgatc ccggaactgc tcggtggccc
    15241 ggacagcatc atgatcgggc gcgtgctatg gcaagagttt ttcaataacc gcgactggcc
    15301 ggtggcctcg gcggtagcga tcatcatgtt gctgctgcta attgtgccga taatgtggtt
    15361 ccacaaacac cagcaaaaaa gcgtgggaga acacggatga ataatttacc ggtagttcgt
    15421 tcgccctggc ggattgtgat tttgctgctg ggctttacct ttctctacgc gccaatgctg
    15481 atgctggtta tctattcgtt taacagctcg aagctggtga cggtgtgggc cggctggtca
    15541 acgcgctggt atggtgagtt attgcgcgat gacgcgatga tgagtgcggt tggttcaagc
    15601 ctgacaattg ctgcctgtgc ggcaacggcg gcggcgatcc tcgggactat tgcggcggtg
    15661 gtgctggtgc gctttggcag gtttcgcgga tcaaatggct ttgcctttat gatcaccgcg
    15721 ccgctggtaa tgccagatgt catcacgggc ttgtcgctgt tgttattatt cgtcgcgctt
    15781 gctcatgcca ttggctggcc tgcggaccgc ggtatgctta ccatctggct ggcgcatgtc
    15841 actttctgta cggcttatgt ggcggtcgtt atttcgtcgc gtctgcggga actggatcgc
    15901 tcgatagaag aagccgcgat ggatctcggt gcgacgccgc tgaaagtgtt tttcgtcatt
    15961 acgctaccga tgatcatgcc cgcgatcatt tctggctggt tactggcttt tactttgtcg
    16021 cttgatgatc tggtgatcgc cagctttgtt tccgggccgg gagccaccac gttaccgatg
    16081 ctggtctttt ccagcgtgcg gatgggggtg aatccggaaa tcaacgccct ggcaacattg
    16141 atcctcggcg cggtcggaat tgtcggattt atcgcctggt atctgatggc gcgcgcagaa
    16201 aaacagcgga tacgcgacat ccaacgtgca agacgtggct gaagacacta aaatttgcca
    16261 acctggctac ataatgccgc gcatgtcgcg gcattgtttt catggaagac gaaacgttgg
    16321 gattttttaa gaaaacatct tcatctcatg ctcgcctgaa tgtgcctgcg ctggtgcagg
    16381 tggcggcgct cgccattatt atgatccgtg gcctcgacgt gctgatgatt ttcaatacgc
    16441 tgggcgtgcg cggtattggc gagttcattc atcgcagcgt acaaacctgg agtttaacgc
    16501 tggtcttttt aagcagtctg gtgctggttt tcattgagat ctggtgtgcg ttttcactgg
    16561 tgaaagggcg tcgctgggcg cgctggctat atctgctgac acaaatcacc gccgcaagtt
    16621 acttgtgggc ggcttcgctg gggtacggtt atccggagct gttcagcatt cccggtgaat
    16681 caaaacgtga aatcttccat agcctgatgc tgcagaagct gccggatatg ctcatcctga
    16741 tgctgctgtt cgttccctcg accagtcggc ggttcttcca gttgcaataa tgtgtataat
    16801 cgtcgcccct gatgatgtga aggtcaatgt atgcagtgcg cactttacga cgcgggtcgc
    16861 tgtcgttcct gtcagtggat aacgcagccg attccagagc aactctccgc taaaaccgcc
    16921 gatcttaaaa atctgctcgc cgattttccg gttgaggaat ggtgcgcgcc ggtgtcaggc
    16981 ccggaacaag ggtttcgtaa taaagccaaa atggtggtga gtggtagcgt tgaaaaaaca
    17041 ctgctcggta tgctgcatcg cgatggcacg ccggaagacc tttgtgactg cccgctttat
    17101 ccagcctcat ttgcgcccgt ttttgcggcg ctaaaaccgt ttatcgcccg agcggggtta
    17161 acgccctaca acgtggcgcg taagcgtggc gaactgaaat acattctgct gactgaaagc
    17221 cagagtgatg gcggcatgat gctgcgcttt gtactgcgtt ctgataccaa gctggcgcaa
    17281 ctgcgtaagg cgctgccgtg gttacacgaa caactaccgc agctgaaagt tattaccgtc
    17341 aatattcagc cggtacatat ggcgattatg gaaggggaga cggagatcta cctgaccgaa
    17401 caacaggcac tggcggagcg ttttaatgac gtaccgctgt ggatccgtcc gcaaagtttc
    17461 ttccagacta atccggcggt cgccagccag ttgtacgcca ccgcgcgcga ctgggtacga
    17521 cagctgccgg ttaaacatat gtgggatctg ttctgcggtg tggggggctt tggtttacac
    17581 tgcgcgacgc ctgacatgca gttaaccggg atcgaaattg cgccagaggc cattgcctgt
    17641 gcaaagcagt cagccgctga actgggctta actcgtttac aatttcaggc gctggactcc
    17701 actcagtttg ccactgctca gggggaggtg ccggagctgg tgctggttaa cccgccgcgc
    17761 cgcggcattg gtaaaccgct gtgtgattat ctttcaacga tggcaccgcg ttttattatc
    17821 tactccagct gtaacgccca aaccatggcg aaagatatcc gcgaactgcc aggttaccgc
    17881 atcgaacggg tacagctttt tgatatgttc ccgcacaccg cgcactatga agtgttgacg
    17941 ctgctggtga agcaataaaa aagccacagg tgcggcttca gattgctgat caggtgcgct
    18001 ttgttcatgc cggatgcggc gtgaacgcct tatccggcct acaaaatcat gctaaatcaa
    18061 tacattgcag agttgatgtg ggccggataa gcgcagcgca tcaggcaatc ttacttttgt
    18121 catcagtctc aattactgcg gaaaccactg gtcattgatt ttctgatatg taccgtcagc
    18181 tttaattgct gccagcgcgt tattcagttt ttccagcaga gctttgttat ccggacgtac
    18241 agcgataccc aggccggtgc cgaagtattg cggatcggtc actttttcag tcgcaacgcc
    18301 cagttgcgga ttggttttca gccattcgtt taccaccgcg gtgtcaccaa ataccccatc
    18361 aatacgacca tttttcagat cgataaaggc attctgataa ctgtcatagg agacagtttt
    18421 cacttccggg tgctgatcct gaatatattt ctggtgcgtc gtaccgtttt ccatcccaat
    18481 gcgtttgcct ttcaggtcgg caaacgtttt gtaggtatct tttttggaaa tcacgacggc
    18541 tgagttttca tagtagggtg tggtaaacga aacctgcttg ctacgctccg gggtgatatc
    18601 cataccggag attacggcgt catattttct gaatttcagt gacgggatca ggctgtcgaa
    18661 cgcgtgatta gtaaaagtac attctgcctg catttgtttg cacaaggctt ttgccagatc
    18721 gatatcaaag ccgacaatct cattattagc acctatagat tcaaagggtg gataggtggc
    18781 tgaaacgcca aaattgattt tctctgcggc agaagcaccg aaagtaaagg aagcaagtaa
    18841 agcggcaaga actaactttt tcatgatgga actcccgtct gtcaatctta tgatttttgg
    18901 ccgtgtctgc ggcatgggat aacaatgcca tcaagtgaat ttatatgcaa taaacatgat
    18961 taaataattt aaatgaaata aaaaaagacg gataacttag tgagttgtcc gtcttcatta
    19021 taagaattta tgcactatgt aggccggata aggcgtccac gccgcatccg gcacaggcac
    19081 cgtgctgatg tctgatgcga cgctggcgcg tcttatcaga cctacaaacc cccccggcga
    19141 atgtacgcag ctacattaat tccgccgttc gaatgccagt gctttgcgct cgatcagacg
    19201 catcatcagc gtcagcaggc cgttgacgac caggtaaata atccctgccg caccgaacac
    19261 cattacatcg taggtgcgtc cgtacaacaa ctggctgtat cccatcactt ccatcagcgt
    19321 aatggtgtaa gccagagagg tacttttgaa caccagcacc acttcgttgg aataagaaga
    19381 gagcgagcgt ttaaaggcat acggcagcag gatcgccagc gtatcttttt tgctcattcc
    19441 cagggcgcta caggactgcc actgaccttc tgggatcgca cgaattgcac cgtaaaacag
    19501 ctgcgtggta tacgccgcac tgttcagcga taacgcaatc agcgcacata accacggttc
    19561 tgacaacaaa tgccacagtg ccggatactc ctgcaacgtc ggaaattggc ccggcccgta
    19621 ataaatcagg aagatctgca ccagcagcgg cgtaccggta aacagcgtga tataaccccg
    19681 caccagccac accagcaccg gcgttttcag cgtcaggatg atggtaaaaa tcaatgccag
    19741 aatcagtgcc acaatcagcg aggcaacggt tagcgtcagg ctggtgtgca gccctttcat
    19801 cagctcgggt aaatactcaa acattagctg ggcctccgct caaaacgtgt cgcgcgcagg
    19861 tcaatgcgtt tgagaatgta ctgactgagc agggtgatca ccaggtaaat cgccgccgcc
    19921 acaatgtacc aggtaaatgg ttcctgggta cgagtagcaa tgcttttggt ttgtaacatt
    19981 aaatcattca cactaatcaa actgaccagc gcggtatctt tcagcagcac cagccactgg
    20041 ttaccgaggc caggcagcgc atgacgacac atctgcggca tcaccagacg gaaaaagata
    20101 gccgatttcg acagccccag cgcctgacca gattcccact ggcccaccgg tacagctttc
    20161 aacgcgccgc gcagcgtttg cgaggcatag gcggcataca gcagtgacag agcgatgaca
    20221 ccacaaagga acggactcac gtcgaagttc tcaatgtcca tctgcaccgg gatctgcacg
    20281 aacccaagat tgatagtgaa gccatccgaa agcgtcagca gtaactgcga ggagccaaaa
    20341 tagataaaca gcactaccag aatttctggc aggccacgca gaatggttac cagcgctgaa
    20401 cctgcccacg cgacaggacg ccatttcgct gactcccata ccgcaaagaa catcgccagc
    20461 gccagcccga caatcaatgc acaaacggca aggccgacgg tcatcccggc ggcgcttgct
    20521 aaaggaaaaa attcattcat caggaattac ttctggaacc atttgttgta gatggtttcg
    20581 taagtgccat ctttcttcac tttttccagc gcagtgttga gtttctgctg cagttcagtg
    20641 ttgccctgac gtaccgcgat gccgaggcca gtaccgaaat aatctttatc ggtcacttta
    20701 tcgcccactg ccgccagttt tgggttatct ttcagccact cagtgaccac tgcggtgtca
    20761 ccaaatacgc tgtcgatacg cccgttttgc agatccagtt ttgcgttctg gtagctgtca
    20821 tacggaacgg tggtgatttc cgggtgctta tccataatga acttctggtg tgtcgtcccg
    20881 ttctgaacgc cgactttttt gcctttcagc tgatcaacac tggtgtattt gccttgctga
    20941 cccacaaaca gggcagagtt gtcatagtac ggggtagtaa acagtacctg cttttcacgc
    21001 tccggagtga tatccatgcc cgccatcact gcttctacgc gacggaattt caggcttggg
    21061 atcaggctgt caaacgcctg gttagagaaa gtacaggttg catcaatctc tttacacagt
    21121 gcctgtgcca ggtcgacgtc aaaaccaacg atctggttgt ttgcatcaat cgattcaaac
    21181 ggaggatagg gggcttcggt agcaaaacga atggtttcgg cagctgtggc ggaaagacta
    21241 aaacctgcaa ttaacgcggc aatcagaact tttttcattg ttgttatccc gaatcttagt
    21301 gagagagata gtttttaaat gcttcggttt gcggctcggt aaagcagctc gcgtcgcctt
    21361 gttctacgat atgaccattt tccatataca ccactcggct ggcggtttta cgcgccactt
    21421 caacttcgtg ggtgacgatc acctgggtaa tattcgtttc tgccagctca cgaatgatgc
    21481 tgacgatttg tgccgtaatt tccgggtcca gtgcggcggt cggttcatcg aacagcagta
    21541 cctgcggttc catcatcaac gcacgggcaa tagcaacacg ctgctgctga ccaccagaaa
    21601 gatgcagcgg gtaacgatcg ctataaggtt tgagacgcag acgttccagc agtttttctg
    21661 cacgggccag cgcctgatct ttactcaacc ccagtacacg gcagggcgct tcaatcaggt
    21721 tttgctgcac ggtcagatgc ggccacaggt tgtattgctg aaacaccatg ccaacgttac
    21781 gacgcaaatc gcgaatcgct ttgtcagagg gtgttttggt gaaatcgaaa tggttgcctg
    21841 caatgttgag cgtaccggag cgcggcatct caagcagatt gagtacacgc agcagcgagc
    21901 ttttacccgc gccgctgggg ccaagtaaca ccagcgtttc gccctgtggg caatccagcg
    21961 tgatatcgaa cagcgcctga tgcgcgccgt agaagcaatt aatgccgttt aattgaatac
    22021 tcattgacac tcgtatactg gcagtctgat agctattgag gtcgaagata gtacctttga
    22081 cagaataatt atgcaatatt tatgctttaa aagttaaaag caaagcgcat tattcaataa
    22141 acatagcaca aaataacggg ggcggtggtc ggtgagcata aatgtcggca ttcctcacga
    22201 aatgccggat aatttacggg gtttattggt tgatcaaggc gttagcgatt ctcgatggac
    22261 tgacggagcg tacccgccgt ggcatgaacg ctaccgccta agtagcgcac atcgtcgatg
    22321 acccaacact ggccttcctg aatcattaac acttcatcct gccagccctg gtcaccctgt
    22381 ttgagatcca cccgcaatgg aatgttacgg gcatcacgat tagggatagt cgatgcactg
    22441 gcaacgtggg cgctgtctgg caaggtggtt cgactggaga acggatcgct gcttaacaat
    22501 tcacgatgac tattatcgcg gctggcatcg ctaagttggg ttgccagttt gtcactcaaa
    22561 taggggcgca gggcggtaat gtcattgctg cgatgttgaa tgcggtagtc gtagaattgt
    22621 tgagccacac tgtcaggccc tccctggaca caggaaccgg tgcgggggcc gttatcttta
    22681 taagctggcg tgacggtggt acaggcactc agcagcagtg cacatggaat aagtagagat
    22741 agtgaacggt agcgcataat catttcctta ttagcttgct gtctgcgtca cactacgacc
    22801 aaacgctatc agattattta atacattgtt tttaatacaa ttctgaaaca atctgaacac
    22861 ctgattgggt ttgagtccat tgctggctac ccactcaagg ttgctcgtag ctgacttact
    22921 taaattaccc aaacaataga cagtgtaaac catttgtgag gttgagcgtg ttatatggct
    22981 gaaatgatca tttaaggtaa atatgatagt tctgaaatgg tggtataaat cctcctgtag
    23041 aggttaagag catctgcaag aagccagttt cacatttata acgcgtgata aatgaatgcg
    23101 gttattgtct aatcattaca aaatttttca catgcgataa gagatcctgc gattaagatg
    23161 attgccaaaa ttacgttgta tttttgaaaa tgagcgaaat ttgatagtcc cagctttgac
    23221 tctcgataat attatttatg ctacaattgt gggtaaagaa tttaagctaa tgaatttgag
    23281 tgaagaataa attaaattgc ttcagtattt cgttaattaa tataaataca aaaggcgttg
    23341 tgcttttata attataaagc gcgaggagtt cggtggatta tcgtttttct tgggtgattt
    23401 ttaatatgat aaacaatata tctgatcaag catcatcatt tccaggcaca caactaaatc
    23461 aaagtgataa tttcctggat tcactgaggg aatttttcgc gatattaaat tccagtcgta
    23521 agggtgagtt gtctacatgg gataccatct acttgcatct gatattagca ataaatgctg
    23581 acagtgatct cataaaaaat gatgtgttgc tcgcggaaaa tattccatca gcgaattacc
    23641 aatttaatac attttttagc aatactttcg agattgatgt taaaaaatat ctgggtaaat
    23701 cagaagataa cgaagttgaa ataaaagcgg gtaatgaaag aatctctatt ggaatacgta
    23761 atgtatcgaa tggaaaactt gaaagacaac agtttctttt cccactggac tatgagaata
    23821 aattacaaga acaattagat aagtatttta cgattgaatc gcacccatta ttatacaggt
    23881 ataccattgg tagtaagatt gcgaatgtga tttttgaaaa gttatacagt cgtattgact
    23941 ttaacaaaga acaatatata tcctttataa aggacgcatt tattcatttt tatgattata
    24001 gcaggcgata tgctattagt gagaatatcg ataaagacgc tgtaacaaac aatatcgcat
    24061 tgatgagcac tttttatgac agtgataata cgtcaggtga ggttttaaat aatgatttta
    24121 cagaagaaga gtcatttgaa actgcgttag atgtagagca tgcaatagtg cttggatttg
    24181 ctgatgataa ttttgaaaca aaacctgttc attatcagga tttattaaca cgatttagtg
    24241 cctttcaaga taccgtgttt aatctgtttc ctgaaatgca ctcttcgcat taccacgata
    24301 tttgtagcgt aagtgtggat atgacaaagg gaacgcaatg catgattcac ttgatggtga
    24361 atgaagaagt tttcatgtct ctgcctgttc cagttgccac gatggtacgt gaagatgcaa
    24421 gtaatttagt caatcttaaa acactactca atgatggttg tttcattaaa tatagtcatt
    24481 ttaatgacgt ggcattaata aagcaaaaca taagtaatct ctatttatca cacactgttg
    24541 tgaatgaaag catattgaaa aaatgttgtt ttgaaaacgg cagtttaggt gatgttaaaa
    24601 taacaaatag caatgtaata aacagtgcat tcaaaaatat ttcgtttaga tctgttaaaa
    24661 ttaataacgt aaatactcat tctttaaaat ttattaactg caattttttc aatgttgata
    24721 tgataagagt taatctcagc aaatgtcttt ttcatgaatg tagtatgcat ggtgtcaaga
    24781 taaaaccctg gttgcctgta aaatggacaa aagaattaat aagtgattat ttgtatggct
    24841 gtttactatc tctgtactct atctgtgcaa gggatatata taatatgaac gccgggaata
    24901 acgtgaaggt tgctgcggat gctttccttg aaattatttt ctctcttaaa aataaatatt
    24961 gtattaaact attaagcgct caggatcggg cgtttatata cgagtttgca agaatgatct
    25021 tcgcttatat taatgacaaa agcattgaga tcttattact ttcttgtttt gcggcggctg
    25081 accaaaaagc aatccagcgg tatagacctc agtcacaaga tggggaagac tttaggagtc
    25141 atttacaata taagttgcct ctttccgctc attaatgttg ttggtgcaac ataataagac
    25201 aggtaagcgg taccctataa tgaaaccaat atcgggttat gcaacggata attgtaaaga
    25261 cttaagtggg tgtttttgac acaatgagat gatttcctaa actgatcaaa accttcttcc
    25321 caacatggca tatcagtccg ataatgcttt agagatcgaa ggcttaacaa acaaggagat
    25381 cgatcatgca attttctaca accccaactc tggaaggcct gaccatcgtt gaatattgcg
    25441 gtgtggtgac cggcgaagcg attttaggtg ccaatatttt ccgtgatttc tttgccggta
    25501 tccgcgatat cgttggcgga cgttccggtg cgtatgaaaa agaactgcgt aaagcacggg
    25561 agatcgcctt tgaggaatta ggctcccagg cgcgggcgct gggggccgat gccgtagttg
    25621 gtattgatat cgactacgaa acggtcgggc aaaacggcag tatgctgatg gttagcgtca
    25681 gcggtacggc ggtgaaaacg cgtcgatgag aagagtcttc tggctggtcg ctgccgctct
    25741 gttactggca gggtgtgcag gcgaaaaagg cattgtcgag aaagagggat atcagcttga
    25801 tacccgacac caggcgcagg cggcgtatcc gcgcattaaa gtgctggtga tccactacac
    25861 cgcagatgat tttgatagct cgctggcgac actgactgat aagcaggtca gctcgcatta
    25921 tctgatccct gcggtgccac cgcgatacaa cggtaaaccg cgcatctggc aactggtgcc
    25981 ggaacaagaa ctggcctggc atgcggggat tagcgcctgg cgcggggcaa cgcgccttaa
    26041 cgacacctct attggcattg agctggaaaa ccgtggctgg caaaaatcat ccggagtgaa
    26101 atattttgcc ccgtttgaac aggcacagat tcaggcgctt attccgctgg cgaaagatat
    26161 tattgcccgt tatcacatca agccggaaaa cgtagtggca catgcggata tcgcaccgca
    26221 gcgcaaagac gatccggggc cattatttcc ttggcagcaa ctggcgcagc aggggattgg
    26281 tgcctggccg gatgcgcagc gggtgaactt ttaccttgcc gggcgcgcgc cgcacacacc
    26341 ggtagatacc gcgtcattgc tggagctttt ggcgcgctac ggttatgacg taaaacctga
    26401 tatgacaccg cgcgagcagc ggcgcgtgat tatggcattc cagatgcatt tccgcccgac
    26461 gttatataac ggcgaagcgg atgcagaaac tcaggcgatt gccgaagcat tgctggagaa
    26521 atacgggcag gattagcgcg gcagttttcc gtggtcgcgt agccaggcgg cagttttctc
    26581 gatgccttca tccagggtga tgaccggctg ataacctaac tcttcctgcg cacgcgtaat
    26641 atccagcgta aagtcaaaat tcaacttgga gacgccgtag tgggtcagcg gcggctcttt
    26701 tgctgacttg cggcctaaac gctccatgct gcgggcgatc atatccagca tcgggtaagg
    26761 gacggaacga atacgacagt caatattcaa ctcgtcgatc agcttctgca cgatgctgcg
    26821 cagtgtgcga tgctcgccgt tggtgatgtt gtacacacgc ccggaaggta gcttatcgca
    26881 ggcttcctgg cttgccagcc acattgcgtg cacggcattt tcatagtagg tcatatcgac
    26941 cagcgcactg ccgccatgcg gtaacagaat actgccgtag tggtgcatca tatgcgccag
    27001 acggggaata aagactttat cgtgcggtcc gaacagactt tgtgggcgca gaatagtaaa
    27061 gcgcgtttgt ggattcgcct gcgaaagcat attgatcact tcttcgctgg ctgctttgct
    27121 gcgggcaaac tcgttggcga agcggtgagg gcgaaaatct tctttaatat tgcgatggtg
    27181 gtgataatcg aagtacaggg agggggaaga gatatgaata aagttacgca caccccaggc
    27241 gacagcccat tcacccaggc ggcgagtggc gcgaacgtta gccagatcga aagcctgttg
    27301 tgtcccccag ggtgaggtaa agctggagca gtgccacagc gtatcaatgc ccgcgagcat
    27361 cactttagct tgtgatgaaa ccagctcggt cagatccgtc ggaacaaact ctgcgcccat
    27421 tttttccagc aatttaccca ttgcttcgtt gcgaccgctc gcacgcacgc tgatgccttt
    27481 ctggcataaa aactctaccg cgtttcgacc taagccgctg gtggcgccgg taaccagtac
    27541 cttcatatca atccactgtt gttgagaaaa tgacgtgcgc attcttccgt gatttccccc
    27601 atgatgcaat gggaaacatg aaagaataac gcaggttttg tcgattaatc tgtgctttgt
    27661 tctgccagtc tggcgatttg ttttgccatt ccgcgaaaaa taaacagatg cgcggggatc
    27721 atcaataacc agtaaaacag ccccggcata ccgtgcggat gccagaaagc gcggacatcg
    27781 atagtacgat agtcaccttt atcttccagg gtaaaacaca gtcgtcccag ccccggcgct
    27841 ttcatgccaa ataacaacgt aagttgcttt ttcggttcaa cgacaatcac tttccagcta
    27901 tccaccgcat cgccggtctg taaatattcg cgctccgggc ggcctttcgc cagattatga
    27961 ccgatcgcgc ggtccatcaa cgcccgtgtc tgccacaaaa tattgccaaa gaaataacgc
    28021 tctttaccgc cgatttggtt caccaccagc cataaagccg caaggctggc ggacgtttta
    28081 acggtaaacc ccgcctgttt ggcgaaataa ccgtactccg gtcgccagcg ggcaaaggcc
    28141 tgagcgtcgt agccccagtc gctggagttg accagttttt cttcctcttt caacgtgcta
    28201 cgtaccgcgt catcgaaagc gatcagccgt tgtgggatga gtgcacgtag cgcggtatca
    28261 tctgccagca gatcgtgttt cagcccctga atcaacgccc tggcggtggt gggcggtacg
    28321 gaagtaatca cattgagaaa ccacaccgaa atccagcggg tggggagggg gatggggatc
    28381 aaccagcggc gcttaccgct caccgccata aaatgttcaa actgttgctg ataactgagc
    28441 acctctggtc cggcggcttc gaagatgcgg tgttcgttgg ctggatggtc taacagcgcc
    28501 accagatagt gcagcaagtt ttccagcgcg atgggcgtgg tgcgtgaacg tacccagcgt
    28561 ggcggcgtta acactggcag gttgtagacc atatcgcgca tgacttcgaa cgccgctgaa
    28621 cctgcgccaa cgataattcc ggcccgaagt tcggtcactg gtacattcgc ttcacgaaga
    28681 atgtccgccg tagcctgacg agctcgcaga tgatccgact gctcatgtgg cggggcctgc
    28741 aacgaactga gaaagattaa ttgcttaact ggtacttcac gtagcgcatc gcggacgttg
    28801 agagccagct ggcgctcctg agcgataaaa tcgccgcctt cgcccatgct gtgcaccaga
    28861 aaatagacgg tatcgatatc ctgcaacagg gccggaaggt tatccggcca gctgagatcg
    28921 actttatggc aactgacgtt tgccagttgc agctttgcaa gcctgtcgac atgacgtgcc
    28981 gccgctagga tctgatgccc ttgctggctg agtgtgcgca ccagatgctg accaatgtag
    29041 ccactggcac cgagaactaa aatgcgttgc ggcacgtctc tctccttaac gcgccaggaa
    29101 tgcgcgccag tgggcggcga cttccgccag ttgttcgcgc gagacgtcaa gatgcgtcac
    29161 caggcggaca atcggcgagg cgttaatcag cacgtttctc gctttcatgt attcgcctaa
    29221 cgcggcagca ttttcttccc cgacgcgaac aaacagcata ttagtgtcct gacgcatcac
    29281 atccgcgcct gcttcacgca gttgctccgc catccaggcg gcgttgtcgt gatcttcctg
    29341 caaccgtgcg acgttatttt tcagggcata catcccggcg gcagccagaa tcccggactg
    29401 acgcatcccg ccaccggcca ttttccgcca gcggatggca cgtttaatgt aatcacgatt
    29461 accgacgagt aatgaaccga ctggcgtccc aagacctttc gacaggcaaa tggtgaacga
    29521 atcacaatat tgcgtgatct ctttcagttc gcagccgtaa gccaccacgg cattaaagat
    29581 gcgcgcaccg tcaacatgca gcgccagatt gcgctcgcgg gtaaattccc atgcttcttt
    29641 caggtattca cgcggcagca ctttgccgtt gtgggtgttt tccagactga gtaatttggt
    29701 gcgggcgaaa tggatatcgt cgggtttgat tttcatcgcc actttatcca gcggtagcgt
    29761 gccgtcggca gccgcgtcga tgggttgcgg ctgaatactg cccagcaccg ctgcgccacc
    29821 ggcttcaaac agatagttat gcgcggcctg accgacaata tactcttcgc cacgttcgca
    29881 gtgactgagc agagcgacca ggttggcctg agtgccggta ggcagaaaaa tggcggcttc
    29941 tttaccggaa agctctgctg catagtcctg cagagcatta acggtagggt cgtctccgta
    30001 aacgtcgtcc ccaaccgggg cggccatcat cgcttcgagc atggcgcggc tcggtcgggt
    30061 aacggtatca ctgcgtaaat caatcatggc atgtccttat tatgacggga aatgccaccc
    30121 tttttacctt agccagttcg ttttcgccag ttcgatcact tcatcgccgc gtccgctgat
    30181 aattgcgcgc aacatataca ggctaaaacc tttggcctgt tcgagtttga tctgcggtgg
    30241 aatggctaat tcttctttgg cgaccaccac atccaccaac accggaccgt cgatagagaa
    30301 ggcgcgttgc agggcttcat caacttcaga ggctttttct acacggatac ccgtaatgcc
    30361 gcacgcttcg gcaatgcggg caaagttggt gtcgtgcagc tcagtaccgt ctgtcaggta
    30421 acctccggcc ttcatctcca tcgccacaaa gcccagcacg ctgttattaa agacgataat
    30481 tttcaccggc agtttcatct gcactactga gaggaaatcg cccatcaaca tgctaaaacc
    30541 gccatcgccg cacatggcga ccacctgacg ttccggctcg gttgcctgcg cacccaacgc
    30601 ctgcggcatg gcgttagcca tcgaaccgtg gttaaacgaa cctaacaggc gacgcttgcc
    30661 gttcattttc agataacgtg ccgcccatac cgttggcgtg ccgacgtcac aggtaaagat
    30721 ggcgtcatcg gcggcaaaat gactaatttg ctgcgccaga tattgcgggt gaatggcttt
    30781 ctcgctcggt ttagctaaat cgtccagccc tttgcgggca tcgcggtaat cttccagcgc
    30841 tttatccaga aacttgcgat cggttttttc ttccaccagt ggcagcaatg cgcgcagggt
    30901 tgatttgata tcgccgacca gcgccatatc taccttgcta tgcgcgccga tgctggctgg
    30961 attaatatca atctgaataa ttttggcatc ggtcgggtag aacgcacggt aggggaactg
    31021 agtaccgagt agcactaacg tgtcggcgtt catcatggta tggaaacctg acgagaatcc
    31081 gattaacccg gtcattccaa catcatacgg attatcgtat tcgacatgct ctttaccgcg
    31141 tagggcatga acaataggtg ctttaatttt cccggcaaac tcaactaact ctttatgcgc
    31201 ccccgcgcag ccgctgccac acatcagggc gatattgctg gaataacgca gcagttgcgc
    31261 cagtttgcgt aactcttctt cttccggcat cacgactggc tgtggcgcat gataccagtg
    31321 cgtggttgcc ccttctggcg caggttttaa cgccacgtcg cccggtaata caacaacgga
    31381 aacgccacgg ttaagcaccg ctttgcgcat agcaattgcc agcacttgtg ggatctgctc
    31441 cgggctggaa accagctcgc aatagtgact acattcgcgg aatagctctt gtgggtgggt
    31501 ttcctggaaa tagccgctgc caatttcgct ggagggaata tgagcggcaa tcgccagtac
    31561 cggaacgtta ttgcggtggc aatcgaatag gccgttgatc aagtgcaggt tgcctgggcc
    31621 gcacgatccg gcacagaccg ccagttctcc tgaaagttgt gcttcagcgc cagcggcaaa
    31681 cgccgccact tcttcatggc gggtggacat ccactcgatg gtgcccatgc gattaagact
    31741 gtcgctaaga ccgttcagag agtcgcccgt gactccccag atgcgtttca cgcctgccga
    31801 ttcgagtgtt ttggcgatat aagctgcaac cgtttgtttc atggttctcc atctcctgaa
    31861 tgtgataacg gtaacaagtt tagttcatct gacggagggg gaagggatgg gagagaaagg
    31921 aggcactaac ggttaaatag cccgatgaaa ggaatatcat cgggcataag gcgattatgc
    31981 gagaaccaaa tccccctgcg gatggcagga gcaggccagt acgtaacctt cagcgatttc
    32041 ggcgtcggtc agcgtcattg tactgctcac cgtatattca ccggaaacca cttttgtctt
    32101 acagcagccg caaacacccg cacggcaggc agcgacaacc ggaacgttat tgctttccag
    32161 cgcctccagt agcgtggtgc caaccggggc gtaaaattct cgtgccggtt gcagtttggt
    32221 gaatttcaga ccgctggtcg ccgcttctgc tactggggtg aagaatttct ctttaaagaa
    32281 acgcgtcacg ccgagcgctt tcacttcctg ctctacccaa tccatatacg gagccgggcc
    32341 gcaggtcatc acggtacgtg aagctaagtc aggtacatct gccagcagtt cgcgagtgag
    32401 acgaccagcg ataaagcctt cggtaacgtt attttctgcc accagcgtta ccggatagtt
    32461 acgccactca tcggcgaaaa taacatcctg cggcgtacgc acgttgtaga tcacccgcac
    32521 atcggcctgt ggacggttct tcgcaagcca gcgacgcatc gacataatcg gcgtgacgcc
    32581 gcagcctgcc gccagcaaca ggaatttatc ttctgcttta tcgtcgcagg taaattcccc
    32641 catcgcgtcc gaaagccaga gataatcacc gcgttttaca tcgcgcgtca gccactggga
    32701 gccgacaccg tcatcaatcc gccgcacggt cagggtgata tattcactca cgcctggcgt
    32761 ggaggaaatg gtgtaagcac gcagcgtttc cgctgagttt cgcacgctga ccagtgcata
    32821 ttgcccggcg cgatatgggt agtgatcgtg gcaaatcagg gaaatcgtcc acacatccgg
    32881 cgtttcttgc gtaatgtgat gaacctgtat ccgccacggg cattgattcg ttggcatcgt
    32941 catcgacaaa ctccttacgc gttcaacagt tgcttcatgt cttcttcaac agtggtgata
    33001 gaacgcaggc cgaatttctc gttcagcacc gccagcaggt ctggtgtcag gaaaccaggt
    33061 gcagtcgggc cggtgacgat atttttcacg cccagagaaa gcagcgtcag cagaatgaca
    33121 atcgctttct gttcaaacca ggagagcacc agcgacagcg gcagatcgtt cacaccacag
    33181 cccagtttct ctgccagagt gacagccaga ataattgctg agtaagcatc gttacattga
    33241 cctgcatcta ccagacgcgg cagaccttcg atatcgccaa actcaagttt gttaaagcga
    33301 tatttaccac aggcgagggt caggatcagg cagtcatccg gcacgctggt ggcgaaatcg
    33361 gtgaagtagt ggcgctcgcc gcgtgcgccg tcacagccac caagcaggaa gatatgacgc
    33421 agtttttcac ggctaaccag atcaatcagc gtatcggctg caccaagcag cgtctggcga
    33481 ccaaagccca cggtgatcag gtgaggaatt tcgctgtacg ggaagcccgc catttgttgc
    33541 gcctgggcga tgaccgcaga gaaatcttcg ccatccagat gacgcacgcc aggccagcca
    33601 acgatgctgc gggtccagat acgatcgtca taagcgccta cggttgggtc gatgatgcag
    33661 ttcgaggtca tcacgatggg gccagggaaa cgagcgaact ccacttgctg attctgccag
    33721 ccgctgccgt agttaccgac cagatgcttg aatttacgca gctccgggta gccatgcgca
    33781 ggcagcattt cgccgtgggt gtagacatta acgcctgtgc cttcggtctg ttccagcagg
    33841 ttgtagagat ctttgagatc gtgaccggaa atcagaatgc atttacccgc cgtcgctttg
    33901 acgttgacct gggttggcgt cgggtgaccg tatttaccgg tttcgcctgc atccagaatg
    33961 ctcatcactt tgaagttcat ctggccgatt tccattgaac actcaagaag cgcgttcata
    34021 tcggcaggcc aggtccccag ccacgccatg attttatggt actgggcata aatatcgttg
    34081 tcgtattgac cgagaacatg cgcgtgttcc atataggccg ccgcaccttt caggccatac
    34141 aggcacagca gacgcaggcc gagaatgttt tcgccaatcg ccgctttatc tttgttaggg
    34201 gtaaattctg ctgcctgacg ttgcagctcg ccgagatcat cgctcaccag ttgcaggtca
    34261 gccatcgggt tatcgacgcg cgcgttggca tctacagcca ggcattgtgc tttcagcgcc
    34321 tcgcgcaagg caatcgcttc acgagcgtag ccgacaatac gcggagaatc gaagttaacg
    34381 ttggtcaggg ttgagaaaaa ggcacgtggc gcgaagctgt caacatcgtg gttgatgatg
    34441 ccgtattcac gcgcttttac cgcccaggca gaaagccctt gcagcgccgc gatgagtaaa
    34501 tcctgaaggt cagaagtttc cgccgtttta ccacacatcc cctgcgcgta tgagcagccg
    34561 ttttctgccg gagtacggat agtttgttca cattgcacac aaaacatgat cacacctttt
    34621 aaagttatat ttaatataca tgtttaaggg taagacgctt aacgcgggga taaaagggat
    34681 ttttcatgca actttaaggg agattgattt agcgcaattt tggcggcagg gaactaccgc
    34741 cagagaggta ttacgcagag aaaaaggcga tgaggatcgg cactaacagg ctaagaataa
    34801 aaccgtgaac aattgccgcc gggaccatat ccagcccgcc agtacgttga agaacgggca
    34861 gggtgaaatc cattgatgtg gcaccgcata agcccagtgc agtagagcgg ctgcggcgaa
    34921 tcagcccagg gatcaacata atagcaatca gttcacgggc cagatcatta aaaaacgccg
    34981 cgctcccgat taccggacca aaagattcgg tcaataaaat accggaaaga gaataccagc
    35041 cgaaaccgga ggccattgcc agcgcggtat tgatggggag atcaagaata aaggcgttaa
    35101 ttaaaccacc aattaatgaa ctgacaacca ccaccacggc gacaatcatt ccccggcgat
    35161 taaggacaat ctgctttaag gtcatgccat tattgcgcaa ctgaatacca acgaggaaaa
    35221 gtagcaaaat taacgtgtat tcactggctt cggtcgcgtg ttgtaagaaa gccagtccac
    35281 tcagaccaat ggcaaaacca atcactacta cgccgcacag ttttagcgac tccagcgcca
    35341 tcgcaatacg cgacgggagt ttttcttgct gatggtggtt gcgccacggc aggcctcgct
    35401 ccagccacat cagggcggca atattacaca gtaaaataac ggtaatactg acggcagaat
    35461 aatgcagaat cgccaacagg ttactggcga ggttatcgag aaacgccaga ctgataccca
    35521 taaaaaagag aataaggtaa accatccagc ttaatagctg attaatagct tttaacgcag
    35581 cttgttggcg aagcggaatg aggtaaccca caatcagggg aaccagaatg attaacagcc
    35641 cagaaaacat gaaaatccag tccttgcaaa gaagaagtcg aaatgcgcga tgacacacta
    35701 ctgaaagcgg aagggcgagt aaagttgcaa ttaaaaggaa atgttatgca tcagaggggg
    35761 atttttaatc ctatctgagt gtttagcatt gttgcgctcc tttcatgtgc aatgccatat
    35821 aaagccactg gcggagggag cgcgcagggg gcagccagtc gccgccgccc cctgcacccc
    35881 cgggctctgg cgaacaaaat cgccgctgcg cggtaccttc agcttatccc cttcggcaac
    35941 cgggtcgggc gcgaggtaac gtcctgtaag acgcgccctc agcccacatc catgtgggct
    36001 gccccggcct tcggggaacg cttcagcgat tttgacgcca ccaatacccg tgcggtttgt
    36061 aatgtcgaag ataaaacaac cagggcagat aaaatgccaa tgctaattaa tcacgctttt
    36121 ccagcagagt ccggtaaatc agaccaccga taatgccgcc gacaattggc accacccaga
    36181 agaaccacag ttgttctaat gcccagccgc cctggaagat agcaaccgcg gtgctgcgcg
    36241 ccgggttaac agaagtgtta gtcaccggaa tactgattaa gtgaatcagg gttaaggcca
    36301 gaccaatagc gatcggcgca aaacctgccg gagcgaattt gtcggttgca ccgtggatca
    36361 ctaacaggaa gcccgcactc aataccagtt caactaccag cgcggaaagc atggaataac
    36421 cgcctggtga atgctcgcca taaccgttag aagcaaaacc gctggctgcc gcgtcaaaac
    36481 ccgttttacc actggcaatt aaatacagca gcgccgctgc aacaataccg ccgacaacct
    36541 gggcaattac gtagccaacg acttcttttg ccggaaaacg tccgccagcc cataaaccaa
    36601 tagtgaccgc cgggttaaaa tgaccaccag aaatatgacc aacagcgaag gccatcgtca
    36661 gaacggtcaa accaaatgcc agcgccacgc cggcaaaacc aatgcctaat tccgggaagc
    36721 ctgcggccag tacagcacta ccacagccac caaaaacaag ccagaaagta ccaaaacatt
    36781 cagctgctaa ttttctgaac atatccacct caattaaaaa ttgaccctgt gaa
//
LOCUS       DAJTQY010000030        36641 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.030, whole genome shotgun sequence.
ACCESSION   DAJTQY010000030 DAJTQY010000000
VERSION     DAJTQY010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36641
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.030"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(157..1392)
                     /locus_tag="OO349_001601"
     CDS             complement(157..1392)
                     /locus_tag="OO349_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCR5866724.1"
                     /translation="MLQQVPTRAFHVIAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYIRHYIAASELQNEVAFTWQGGEPTLLGLEFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENNFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAVRDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLMVVNK"
     gene            complement(1539..1615)
                     /locus_tag="OO349_001602"
     tRNA            complement(1539..1615)
                     /locus_tag="OO349_001602"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1579..1581),aa:Pro,seq:tgg)
     gene            complement(1658..1744)
                     /locus_tag="OO349_001603"
     tRNA            complement(1658..1744)
                     /locus_tag="OO349_001603"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1708..1710),aa:Leu,seq:cag)
     gene            complement(1765..1840)
                     /locus_tag="OO349_001604"
     tRNA            complement(1765..1840)
                     /locus_tag="OO349_001604"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1805..1807),aa:His,seq:gtg)
     gene            complement(1899..1975)
                     /locus_tag="OO349_001605"
     tRNA            complement(1899..1975)
                     /locus_tag="OO349_001605"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1939..1941),aa:Arg,seq:ccg)
     gene            complement(2078..3463)
                     /locus_tag="OO349_001606"
     CDS             complement(2078..3463)
                     /locus_tag="OO349_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5866725.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWSPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(3654..4394)
                     /gene="rffM"
                     /locus_tag="OO349_001607"
     CDS             complement(3654..4394)
                     /gene="rffM"
                     /locus_tag="OO349_001607"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HCR5866726.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(4397..5749)
                     /gene="wzyE"
                     /locus_tag="OO349_001608"
     CDS             complement(4397..5749)
                     /gene="wzyE"
                     /locus_tag="OO349_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HCR5866727.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQVLLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(5746..6825)
                     /gene="rffT"
                     /locus_tag="OO349_001609"
                     /gene_synonym="wecF"
     CDS             complement(5746..6825)
                     /gene="rffT"
                     /locus_tag="OO349_001609"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HCR5866728.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNMMANDRQREGKMTILVGNSGDRSNEHVAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASADKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(6822..8072)
                     /gene="wzxE"
                     /locus_tag="OO349_001610"
     CDS             complement(6822..8072)
                     /gene="wzxE"
                     /locus_tag="OO349_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HCR5866729.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(8074..9204)
                     /gene="rffA"
                     /locus_tag="OO349_001611"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(8074..9204)
                     /gene="rffA"
                     /locus_tag="OO349_001611"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HCR5866730.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDTLAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGEHFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(9209..9883)
                     /gene="rffC"
                     /locus_tag="OO349_001612"
                     /gene_synonym="wecD"
     CDS             complement(9209..9883)
                     /gene="rffC"
                     /locus_tag="OO349_001612"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HCR5866731.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTSDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(9861..10742)
                     /gene="rfbA"
                     /locus_tag="OO349_001613"
     CDS             complement(9861..10742)
                     /gene="rfbA"
                     /locus_tag="OO349_001613"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCR5866732.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(10761..11828)
                     /gene="rffG"
                     /locus_tag="OO349_001614"
     CDS             complement(10761..11828)
                     /gene="rffG"
                     /locus_tag="OO349_001614"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCR5866733.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELASN
                     KPHGVAHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(11828..13090)
                     /gene="wecC"
                     /locus_tag="OO349_001615"
     CDS             complement(11828..13090)
                     /gene="wecC"
                     /locus_tag="OO349_001615"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HCR5866734.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(13087..14217)
                     /gene="wecB"
                     /locus_tag="OO349_001616"
     CDS             complement(13087..14217)
                     /gene="wecB"
                     /locus_tag="OO349_001616"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HCR5866735.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGALYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIAPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(14273..15319)
                     /gene="wzzE"
                     /locus_tag="OO349_001617"
     CDS             complement(14273..15319)
                     /gene="wzzE"
                     /locus_tag="OO349_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HCR5866736.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(15331..16434)
                     /gene="wecA"
                     /locus_tag="OO349_001618"
     CDS             complement(15331..16434)
                     /gene="wecA"
                     /locus_tag="OO349_001618"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HCR5866737.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(16674..17933)
                     /gene="rho"
                     /locus_tag="OO349_001619"
     CDS             complement(16674..17933)
                     /gene="rho"
                     /locus_tag="OO349_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HCR5866738.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(18018..18119)
                     /gene="rhoL"
                     /locus_tag="OO349_001620"
     CDS             complement(18018..18119)
                     /gene="rhoL"
                     /locus_tag="OO349_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HCR5866739.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(18260..18589)
                     /gene="trxA"
                     /locus_tag="OO349_001621"
     CDS             complement(18260..18589)
                     /gene="trxA"
                     /locus_tag="OO349_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HCR5866740.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            18720..19985
                     /gene="rhlB"
                     /locus_tag="OO349_001622"
     CDS             18720..19985
                     /gene="rhlB"
                     /locus_tag="OO349_001622"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HCR5866741.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            20119..21603
                     /gene="gppA"
                     /locus_tag="OO349_001623"
     CDS             20119..21603
                     /gene="gppA"
                     /locus_tag="OO349_001623"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HCR5866742.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENDLSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQENQWQSYVHWPLEVH"
     gene            complement(21650..23671)
                     /gene="rep"
                     /locus_tag="OO349_001624"
     CDS             complement(21650..23671)
                     /gene="rep"
                     /locus_tag="OO349_001624"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HCR5866743.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEAHGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNYQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSTKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            23758..24039
                     /gene="ppiC"
                     /locus_tag="OO349_001625"
     CDS             23758..24039
                     /gene="ppiC"
                     /locus_tag="OO349_001625"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HCR5866744.1"
                     /translation="MAKTAAALHILVKEEKLALDLLVQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(24085..25560)
                     /gene="ilvC"
                     /locus_tag="OO349_001626"
     CDS             complement(24085..25560)
                     /gene="ilvC"
                     /locus_tag="OO349_001626"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HCR5866745.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            25710..26600
                     /gene="ilvY"
                     /locus_tag="OO349_001627"
     CDS             25710..26600
                     /gene="ilvY"
                     /locus_tag="OO349_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HCR5866746.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPLLYGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(26597..28141)
                     /gene="ilvA"
                     /locus_tag="OO349_001628"
     CDS             complement(26597..28141)
                     /gene="ilvA"
                     /locus_tag="OO349_001628"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HCR5866747.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(28144..29994)
                     /gene="ilvD"
                     /locus_tag="OO349_001629"
     CDS             complement(28144..29994)
                     /gene="ilvD"
                     /locus_tag="OO349_001629"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HCR5866748.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALCAYASLATSADKGAVRDKSKLGG"
     gene            complement(30059..30988)
                     /gene="ilvE"
                     /locus_tag="OO349_001630"
     CDS             complement(30059..30988)
                     /gene="ilvE"
                     /locus_tag="OO349_001630"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HCR5866749.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGNVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQIGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(31008..31271)
                     /gene="ilvM"
                     /locus_tag="OO349_001631"
     CDS             complement(31008..31271)
                     /gene="ilvM"
                     /locus_tag="OO349_001631"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HCR5866750.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(31268..32914)
                     /locus_tag="OO349_001632"
     CDS             complement(31268..32914)
                     /locus_tag="OO349_001632"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HCR5866751.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQHC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(32917..32967)
                     /locus_tag="OO349_001633"
     CDS             complement(32917..32967)
                     /locus_tag="OO349_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5866752.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(33053..33151)
                     /gene="ilvL"
                     /locus_tag="OO349_001634"
     CDS             complement(33053..33151)
                     /gene="ilvL"
                     /locus_tag="OO349_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HCR5866753.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            33503..35023
                     /locus_tag="OO349_001635"
     CDS             33503..35023
                     /locus_tag="OO349_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HCR5866754.1"
                     /translation="MSLSIVHTRAALEVNAPPITVEVHISKGLPGLTMVGLTETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKNTLERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGESSATVKQRVMAARERQFKRQNKLNAWLDSPEIRQFCKLESEDAMWLEET
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(35048..35386)
                     /gene="maoP"
                     /locus_tag="OO349_001636"
     CDS             complement(35048..35386)
                     /gene="maoP"
                     /locus_tag="OO349_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HCR5866755.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            35505..36344
                     /gene="hdfR"
                     /locus_tag="OO349_001637"
     CDS             35505..36344
                     /gene="hdfR"
                     /locus_tag="OO349_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HCR5866756.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(36440..36515)
                     /locus_tag="OO349_001638"
     tRNA            complement(36440..36515)
                     /locus_tag="OO349_001638"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36480..36482),aa:Trp,seq:cca)
     gene            complement(36524..36600)
                     /locus_tag="OO349_001639"
     tRNA            complement(36524..36600)
                     /locus_tag="OO349_001639"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36564..36566),aa:Asp,seq:gtc)
BASE COUNT         8945 a        10035 c         9230 g         8431 t
ORIGIN      
        1 tgccaccgca cgtaaaggct ttgccggata cgatcaccca aaccagtatc cacagaacgc
       61 tgcaatttat tgaatttgca cgatcatgta ggccggataa ggcgtttacg ccgcatccgg
      121 caatcaaccg caggcggctt ccaaatggcc gcttttttac ttattcacca ccatcaaatg
      181 tgcctgcata atgtcactgg ccgggcgacc gtgcgccagc aaatcagcca ttgctttaag
      241 atatggcggt agatggcgga aataacgctg atacccggca cacaaataat tcagccccgg
      301 tttgccgctg gcatcgagca tgaagcggtg tttcgggcag cctccccaac atgcttttaa
      361 cacgttacaa ctgcgacact gcgccggtaa ttgcttaaat ttatcttcac caaacacctg
      421 ctgttgcggg gaatcgatca tttctgcaat tgtttgctga tgcatattcc ccagccgata
      481 ttgcggataa acatagtgat cgcaggcgta aacgtcgccg ttgtgctcaa caatcaccga
      541 gcgcccacag gttggctgat gatggcaaac cgcacccggc gcaccgacaa aattggcaaa
      601 cgcccattcg atattcatca cgaaaatctt gccgacgtcg cgtttgatcc agtggtcaaa
      661 aatcgccacc agaaattcac caaattcatc ggggcgcaca gaccattccg tcagttcccc
      721 ctgaatatca ccaggcgcat gtagtttcag tccatcgcgg acagctgttt catcagccag
      781 gcgctcgacc accggaataa actggatgaa ttcgactccc gcatcgcaca aaaaatcata
      841 aacctgtaac ggttgctgcg cgctggtgcg gttgacgcag accagcacgt tatagtcgac
      901 atgatgtttt tgcaggagcg tcagggcacg catcaccagt ttatgcgtgg gtctgccgcc
      961 tttggtcacg cgatattgat tgtggatctc agccggacca tccagcgata acccaacaag
     1021 aaaattattt tctgccagaa atgcacacca ttcgtcatcg agcagcacgc cgttagtctg
     1081 gaaactgtta cttatcttcc tgccagcacc atatttcgcc tgtagcgcca cggcacggcg
     1141 gtaaaactcc agcccgagta gcgttggttc gccgccctgc caggtaaaag ccacttcgtt
     1201 ttgcagttcg ctggcggcga tatagtgacg gatatacgct tccagcgtgt catcgtccat
     1261 atgcgtgact ggcttttcgc ggtaaaggga ttgtttttcg agataaaaac agtagtcaca
     1321 gttcagatta caatcggaac cgctcggttt cgctatcaca tgaaaagcac gcgttggaac
     1381 ctgttgcagc atggtcgctc cttgcctgaa gtcatccttc aatacgttaa gggcgaaatc
     1441 ataccgtggg gcaatgagtt attacgggcg tttattgcga agggagggtg acgaaatgca
     1501 cagaaaacaa aaaaccccgc cgaagcgggg ttcaaaattg gtcggcgaga gaggattcga
     1561 acctccgacc cactggtccc aaaccagttg cgctaccaag ctgcgctact cgccgaaata
     1621 ctgctttttg aatttttagt tcaattcttt aaagtcgtgg tgcgaggggg gggacttgaa
     1681 cccccacgtc cgtaaggaca ctaacacctg aagctagcgc gtctaccaat tccgccacct
     1741 tcgcattgtc acaacttcta ataatggggt ggctaatggg attcgaaccc acgacaactg
     1801 gaatcacaat ccagggctct accaactgag ctatagccac cactacaaat cttgttacgc
     1861 ggtattacta ccaccgcagc tcaagcgccg gtactaaatg gcgcgcccga caggattcga
     1921 acctgagacc tctgcctccg gagggcagcg ctctatccag ctgagctacg ggcgcttagc
     1981 gccgttgcgg gggtggataa tacggacttc ccacccctct gtctagtccc tttttaaata
     2041 aattgctcgt ttggttacgg tttgtgcgtt ttgctgctta ttcctccagt ttatgcgctt
     2101 taccgtggcg attaagccca aaaactttat aaatcgccgt caccgccagc ataaagatga
     2161 tgccgacaaa cagcgacata cgcgtatctt cattaaagta catgccgatc aaaacgcaaa
     2221 tcaggaatgc cattgttacg tagttggccc acgggaacag gattgagcgg aacggatggc
     2281 tggcaatcgc cgctttatgt gcacgccgaa aacgcagctg gcttatcaat atcacaaacc
     2341 atggcaccat ccccggaagc acgctggcac tgtagacgta gacaaacaca cgctgcggat
     2401 tgggaatgat gtagttcagg catgagccaa ttagcagaat agcaatagat actgccacac
     2461 ccgcaaccgg tacgccgtga cgggaaactt ttgccattgc cgccggtaac tgacggtttt
     2521 tcgccagtgc gtagagcata cgtccgcaac tgtacatgcc gctgttacag ccagagagcg
     2581 cagccgtcag caccacaaag ttgataatgc ccgccgctgc ggtaataccg attttggcaa
     2641 aagtcagtac gaacgggctg ccgttgctgc caatttcatt ccacgggaag atggtgacga
     2701 taacgaaaat cgcgcctacg tagaaaatca ggatccgcca cagcactttg cctacggcac
     2761 tgcgcagcgt cacctgcgga ttcttcgctt caccggcagt aatgccaatc agctccacgc
     2821 cctggtagga cgccaccaca atacacagag cggtcaggaa ccctttccag ccacccgcaa
     2881 agaaaccgcc atgctctgtg agattgctaa aaccaatcga ctgcccgcca ttgccaaagc
     2941 caaagaaaat cacgcccagg ccaatgacaa tcatcacgat aatcgtggtg actttgatca
     3001 tcgcgaacca gaactcgatt tcgccgtaca accgcaccgc cgccagattc gccaacgcca
     3061 ccagcgccac cgcgatcaat gcgggtatcc actgcgccat ctccggggac caaaactgga
     3121 cataaacgcc aatggcggtg atttctgaga tccccaccgc catccacata aaccagtaag
     3181 accaggcggt gagatagcca aaaaacgggc tcatataacg atgcgcataa acggcgaacg
     3241 aaccggtaac cggttcgagg aacaacattt cgcccattga acgcatgatg aaaaaaacga
     3301 acagcccggc gatgatatag gccaacaata cggatggccc ggcccatttc agggtactgg
     3361 cggcccccat aaacaggccg acgccaatgg tgccccccag ggcgatgagt tcgatatgtc
     3421 gagcttccag cccacgctgt agctctggtt tgttatctgc cataaatcct cgtgttgtgt
     3481 ttgcatgctt tccggtgtta ccggttatcg ttatgggtac atcgagtgtt gcgaatgttt
     3541 tcgtaattca ggagaaatgg caaataaagc attaaaaatt tgaatgcttt gtgtaataaa
     3601 aaagcagaca ggcgacgaag tgaccactcc gtcgctttac aaagagagga aaatcagagg
     3661 ttgccggtgt agtgccagcg taaataacgc agcaaacgaa gctgacgctt aatgcggctc
     3721 ggctgcgaaa gcaggcggta gagccactcc agtcccagcg tttgccagat tttcggtgcg
     3781 cgttttacgt gaccggtgaa aacatcgtaa gtcccgccaa cgcccatata cagcgcatct
     3841 ggatgcacca gacggcagtc gcgcatgaag atctcctgct ttggcgatcc catcgcaacg
     3901 gtgacgattt gcgctccgct ggcatgaatg cgttcaaaca gcgcctgacg ctgctcgggt
     3961 ttaaaataac catcctgact gccaacgata ttcacattcc attggttgcg cagtttagct
     4021 tcagtttgcg ccagcacttc aggtttaccg cccacaagaa ataccggcgt cccttctttg
     4081 cctgcgcgcg ccatcagctc ttcccagaga tcggcaccgg caacgcggga aacctgcgcc
     4141 tgcgggtact ttttacgtac tgaacgtaca acgctgatgc catccgcata tttaaattcg
     4201 gcagcgttaa ttaactccct gacctcggcg ttatcttcaa tggtcagcat tttttcggca
     4261 ttaatggcaa ccagcgttcc ctgcttaagc tgcccgtcag caaacagata atcgagggca
     4321 tgctgcatat cacgccaacc aatcaactgt aagccacgca gcgtataggt cggtgccgtg
     4381 gtgttgttat tcattgttat ccttcaacct gcgtccggag cgatgatttt gtacgtttat
     4441 gaatgagtcc ggcgctttca aaaagccagt acaacagttt tgcgatcatc agacatgcgc
     4501 cgaagaccac gataaaaaag actacgcgtg agacaaacga atccagccct tcacgtgcca
     4561 gcacgatcat attgaagatc gccccaaagc agaaactgtg caatatcgca gccttatagc
     4621 gattagtctc gcggttgccc agctcataca gccagtcgaa ccatttgatg atcagtccaa
     4681 ccacaatcgc cccgagtggg atgaacaacg cgccgcccat caccaccagt gagcctataa
     4741 gcgtaggcga gatcgccagt ccggagtggt tattcagtac ttcccaggta aagtaattgg
     4801 ctgagttcag caccatactc gggcgccccg gccacagcca ggaagggata aagacataga
     4861 aatcgcggac aattggagcc aggccctgga agtcgatgtt gtcgtagttc tgcaacagca
     4921 acgccagatt ctcccacggt gaaaaggtgt cgcgagtgag atagagaaac gtatagaacg
     4981 cttcatcgcc gctcacattc attccatagc gttttaacgc cagccagaac atgccaacaa
     5041 tccccagcac gcccgccgcc gccagcatcc acaacgaaat ccagccgcga ataatgccaa
     5101 taaacaggaa gatagcgaat gcgatgatga tattggcgcg agtgccgccg acaatcatat
     5161 aagtcagcaa gccaaaggcg accgtgctga cgaggaaaaa cagccacgct ttgctgtcct
     5221 ggcgcagaaa gtagaccacc agcatcgccg ggatgaaaaa gtaaaagaag cgttttaacg
     5281 ccacgccgga gacttcactg gaaaagatct gactgtagga gttaagccgg aacagcaaaa
     5341 agccgttgtg catgaagaag atgccgacgc ttaccagcgc gatacccatc aggatcaccc
     5401 acgtaagatt ggtctccacg cggttcatgg taaacagcgg acggcgcggt acatcagcaa
     5461 cgcgtttgcg taggcgggtt ttgtaggtga catagtaaac cgcgtagaag cagcccgcag
     5521 aaagcaacac ctgcaacaag atttctggcg gcgcgacacc aacatcaaag cgaaatacca
     5581 gcacgctggt cagcgggaag ccgaagaaaa aggtgagcaa aaacagcaat gaaaagaaga
     5641 cattgaagtt aaagcgcaca cggcgaaact caaaccaggt cagcgtggca ataaacagcg
     5701 tgcagagcag ccagaccaca aacaggccac tgaattgcag cagactcatg cgacctcccc
     5761 ggcggcaatc gccaacgccc gctgccagcc ttgtagatag ttagggctaa agaaggcaat
     5821 ggtgttttta tccgccgacg ccaactggcg ctgcgcttca cgcacaatat cctcgttgag
     5881 atcgtcggta gtaaacagca ctggcaaatg ctgttccgtc atatcctgcc agaacggatt
     5941 ttccctgtta agcacacaag gaatgcccgc ctgaatcagt aagcaaagcg taccaatgcc
     6001 ctgctggcgg gcaaaaataa agtaaccaag atcgcactga cgaagtagcg ccagataggc
     6061 gtcaaattcc agtttttcgc tcaaaatttg cagattttct tcgctgaata actccagccc
     6121 cgcctgacga acttcctcaa tgtacgcttc gttattaggc ggatatccca tcggcaccac
     6181 cacttttacc gtatcgccaa attgctgatg aacggcacgt aaagcggcga catgctcatt
     6241 gctgcggtcg ccggagttcc ccaccagaat ggtcattttt ccttcacgtt gccgatcgtt
     6301 cgccatcata ttgagagaag ggtccatccg cgtcgggaag tacagcagtt ctccccgtac
     6361 ctttgggtgc gttttggcaa aaaagctcaa atcgccgcgg gtggcaaata cacagccgac
     6421 tcgcttttgc gccaggcgac gtagtgggta aaaaagctta tatctcaagc cactggaaag
     6481 ctcatacagg tctgcccccc agatatgcca gaaaaactgg ctgggcttaa tgccaccact
     6541 cagcagagcc agccacagtg tgggattgaa ctgaccgtgg aaaaaaaatc gctgctgacg
     6601 gttagctttt gctttcgcga tgaccgcttc cgccagcgat tttttcccag ggaaaaattg
     6661 cacagaaagc gccggacagc tatcacttaa gccgtcgtcc ttgccaacaa ccataaactc
     6721 gcgcgcgtgc tcgctcgtcg cggccagcgc gtcattgaaa aaccgcaaaa cggttcggtt
     6781 atggtgaggg atatccgatc ccagtacgtg aatcagtaca gtcatgcccg cctacgccag
     6841 agtaaaaaca cgccacaaca aagagaaaaa tagacgatat aagttgccat atatgcctgc
     6901 gccgcgccca gtgcaccatg cgcagggatt agccagtggg caaataccat caataaagtg
     6961 aactggctga cttccgccag aatataaaac cgcagtgacg ctttggcgat caccagataa
     7021 ccaaagacat aagcgcccac ttttaacaca tcacccacta actgccaggc aaagagatcg
     7081 cgcatagcgg taaatttatt cgacaacagc aaccagatag caaaatcacg cagcaaccag
     7141 acggtaaaac tcgctgccgc cactgccggt aagacgaatt tcagcgattt aaccacttcc
     7201 cgggtgatat cgcgcttttc cgttagccgc gacaacgtgg gcagcaaata tacgctgaac
     7261 gatgccgtaa taaattgcag gtaggcatcg gaaatactgc tcaccccttg ccagatcccc
     7321 acttcatccc agctatactg cgccgccagc agtttacgca tcatgatgta agcaacaggc
     7381 aaggtcaccg acgtaatcaa cgccatgagc gtaaatttgc tcaactgccc tgccagaccg
     7441 ttatcccagc tgggtttcag atagcttaac gggatgacac cacgtttaat caacatgatg
     7501 gcggcaggaa ttaccaccag cgcgggaatc agcgccagac ccagcaacgc cccttcataa
     7561 ccgcccaaac ggtaactgac gtagtacgcg agaacgccaa tcaagctgcc gacaatcagc
     7621 gataacgcat tacctgcggc atcgcgaaag cctttcatca gcgccagtaa caggttgccc
     7681 caggcgatcc ccatttgcac cagcgccact aaacgcacca gcccctgata gtcggtatta
     7741 ccaaacaatc cctggctgat tggcgcagct gccagcacaa aaaccagcgc catcagcgta
     7801 gagaaaccaa gtaccatcgc tgatgaagtg ccgaccacgc ggcgcagctg ttgcggatta
     7861 tcatggtact gggcaacgta tttggttaca ccgttaaaga tgccagcccc ggcaagcacg
     7921 ccgagcacgg taatcaactg gcggaaattt gccgccagcc caagccccgc cggaccaaat
     7981 gacaccgcca gcaacttacc gaccagtaac ccggcaccaa tcttgaccag tgtactggcc
     8041 gccgtccaca aggacgcttt tgccaacgac atatcaggag aagtagttca acaaagtcgc
     8101 aattaccgta cgctgattga cgggcgacag gttgtagaac agcggcaggc gcagcaggcg
     8161 ctcgctctct ttggtggtgt agcgatcttc accgtggaac tcaccaaagt gttcccccgc
     8221 agggcaaccg tgcagcggaa tgtaatgaaa caccgccatg atttccgctt ctttcagaaa
     8281 gttaatcaac gcgctccggt catcaatatc ccgcagttta atgtagaaca tatgcgcgtt
     8341 ctgcacgcag ccatcgggaa tcgacggcag ctcgatacgc ccggctttcg ccagaggcgc
     8401 taacgtatcg tagtagtttt gccacagcgc cagacgttgc tggttgatac gatccgctgc
     8461 ttccagttgc gcccacaggt acgcagcttg cagatcggac atcaaatagc tggagccaat
     8521 atcgcgccag gtatatttat cgacctgacc acggaagaac tggctgcggt tagtgccctt
     8581 ttcacggatg atctcggctc gttcgattaa cgctttatcg ttaatcagcg tcgcgccgcc
     8641 ttcaccaccc gccgtgtagt ttttggtttc atggaagcta aagcagccaa tatgaccaat
     8701 ggttcccagt gcacgccctt tgtaagtgga catcacgccc tgagcggcat cttccaccac
     8761 aaacaaatta tgctttttcg ccaacgccat aatggtgtcc atttcgcagg ccacacccgc
     8821 gtaatggacc ggcacgataa cgcgcgtttt gtcggtgatc gctgcttcaa tcaacgtttc
     8881 gtcgatgttc atggtgtccg ggcgaacatc cacaaagacg attttcgcgc cacgcagcac
     8941 aaaggcattg gcggtggaga caaaggtgta gctcggcatg atcacttcat cgccaggctg
     9001 gatatcgagc agcagcgccg ccatctccag cgaagcggtg caggacggcg tcagtaacac
     9061 tttggcgctg ccaaaacgtt gctccagcca ctgctggcag cgacgggtaa aaccgccatc
     9121 gccacacagt ttgccgctac ccattgccga ctgcatatag tcgagttcgg ttcccaccac
     9181 cggcggtgcg ttaaatggaa tcatgtgatc acctgtataa ccagtacgcg gtgctttcta
     9241 cattcgcacc actttgtatg tatcgtttaa gcgcggcggt gttgcccatt tgggtcgcca
     9301 cccgcaaagt tgttttaccg cgagcatacg cccagtttag cgccgtttgc atcagctcag
     9361 cacctgcacc gcgtccagcc agcaggccaa ttcgcgcatc tgtcgcattg agttcccgta
     9421 aagagacata gccgcgaata tcgccggacg ccgcacgtag aatcagacat tgatgatcaa
     9481 aggtgccgcg cacggcattt tcaatccact gtgcataaaa gcgaccgctg gcgtcaggcg
     9541 cataccacgg cgcacgaaaa cggctttgcg caaatgcggc gctagctaac tgacgtaatg
     9601 cgggaatatc ggtctcttgt gccacgacag caccgctatc actgacattg ttcacgggta
     9661 gcgccaaatc aacttcacct tctaccaggg agaatcccag ctgttgcagg gcatccagtt
     9721 cccccgtatt tgatgccgca attttggcct gcacccgtga ccatggcgct aaggcgtctg
     9781 acgtcaggag cggtgcttca gacgtaatgc gcacgatggc gctgttaaca ccaaagaagg
     9841 cgttttccca ggttagtggc tcaatactgg cgcggacggg cacgaagtaa ctccagcaga
     9901 tattggccgt agccagtttt cgctaatgaa ctggcagcac gcttcacacc ctcgtcatcg
     9961 agccagccgt tacgccaggc gatctcttcc aggcaggcaa tcttaaagcc ctggcgtttt
    10021 tctactgtct gcacaaaagt gctggcctca atcaggctgt cgtgagtgcc ggtatccagc
    10081 caggcaaatc cgcgcccgag cagttcaacg gtcaggttgc ccgcctcgag gtacatctgg
    10141 ttgatggagg taatctccag ttcaccgcgc tccgacggct tcacctgctt tgcgtactcc
    10201 acgactttac tgtcgtagaa ataaagcccg gtcaccgccc agtttgactt cggctgtttt
    10261 ggcttttctt caagcgagat agcgcggaaa ttgtcgtcaa attccaccac gccaaagcgt
    10321 tccgggtcca tcacctgata gccaaaaacc gtcgcccctt ccgtgcgcgc cgcaacatga
    10381 cgcagcttcg gactgaagcc ctgaccgaag aagatgttat cgcccagcac cagacaagaa
    10441 ggttcaccat taaggaaggt ttcaccgatg ataaaggcct gcgccagacc gtccgggctg
    10501 ggctgttcgg catattccag ctggataccg aactcactac catcgcccag caggcgctgg
    10561 aaataacctt tatcttccgg cgtagtgatg atgagaattt cgcggatacc ggccagcatc
    10621 agcaccgaca gcggatagta aatcattggc ttatcgtaaa tcggcaacag ttgcttcgat
    10681 acgccgcgcg taatcggatg caatcgggta ccggaaccgc ccgccaggat aatacccttc
    10741 atgtttgcct ccgctgaaaa ttagcctttc agacctaaac gctcaccctg atagctgccg
    10801 tcctgcacct gcttccacca gctttcatta gccagatacc actgaaccgt tttacgcatt
    10861 ccactttcaa aggtttcctg cggcagccag ccaagttcac gggcaatttt cgaagcatca
    10921 atggcataac gcagatcatg ccccggacgg tcagcgacaa aggtgatcag gtcacgatag
    10981 tgcgccacgc cgtgcggctt gttcgaagcc agttcttcca gcagctcgca aatggtttcc
    11041 acaacatcga gattcttacg ctcgttgtga ccaccaatat tataggtttc accgactttc
    11101 ccggtggtcg ccacgcaata cagcgcgcgg gcgtgatctt ccacatacag ccagtcacgg
    11161 atttgctgcc cgttgccata taccggcagc ggtttacccg ccagcgcgtt gaggatcatc
    11221 agcgggatca gtttttccgg aaagtggtaa ggaccgtagt tattcgagca gttggtgatc
    11281 agcgtcggca gaccgtaggt ccgcagccag gcgcgcacca gatggtcgct gctggctttt
    11341 gacgcggaat aagggctgct cggcgcatac ggcgtggttt cggtgaagaa atcatccgtc
    11401 gagtgcaggt caccatatac ttcgtcggtg gagatatgat gaaaacggaa cgctgatttt
    11461 ttatcttccg tcagcgtatt ccagtaagcc cgcgccgctt caagcaatgt ataagtcccg
    11521 acaatgttgg tttcaataaa cgctgccggg ccgtcaatag aacggtcaac atggctttcg
    11581 gctgccagat gcatgacaca gtctggctga tgctcagtga atacgcgtgc cagttctgcc
    11641 cgatcgcaga tatcaacttt ctcaaaggca aagcgctcgc tttgcgcgac cggtgccagc
    11701 gacatcaggt ttccggcgta ggtcagctta tcgaccacta ccaccgcatc gctcgtttcg
    11761 ttgatgatat aacgcaccag cgccgagcca ataaacccgg caccacctgt tatcagaatt
    11821 tttctcatca gcgccagact cctttggcat cgacgacata ctgctgatgg acattgtcgc
    11881 cattgataac tttgaactga ctatgatcga ccagcatcac cagcacgtct gccgttgcca
    11941 gcgcctcgtc aagctgcgcc agagtacaaa gcccggtcag ttttttcggc aactggtgga
    12001 tgttaggctc aacaaccagc gtttcgccgc tgtgccactg ggcgatcagt tcagcgattt
    12061 ccatcgccgg gctttcgcgc aggtcatcaa tattcggttt aaacgccaga ccaaagcagg
    12121 cgattttcag ttcactggcg cgtttatcgg tagcagcaag gcagtcggca accgccgctt
    12181 tcacctgatc gataacccag aacggtttgt gatcgttaac ttcgcgcgcg gtacggataa
    12241 gccgcgcctg ctgggggttc tgtgccacga taaaccacgg atcaacagca atgcagtgac
    12301 cgcccacgcc agggccaggc tgaagaatat taacgcgagg gtgacgattc gccaggcgaa
    12361 tcagttccca gacgttaatc ccctgatcgg cacaaatcag cgacaattca ttagcaaaag
    12421 cgatattcac atcgcggaag ctgttttcgg tgagcttaca catttccgcc gtccgcgagt
    12481 tagtgacgac acactcacct tcgaggaaaa ttttgtacaa ttcgctggcg cgggccgaac
    12541 aaaccggcgt cataccacca atcacgcgat cgtttttaat cagctcgacc ataacctggc
    12601 ccggtaatac acgttccggg cagtaagcaa tgttgacgtc cgcctgctcg cccacctgct
    12661 gtgggaaagt cagatccggg cgcatctctg ctaaccattc tgccatcttc tcggttgacc
    12721 ccaccggcga ggtggattca aggatcacca gcgcgccttt tttcagcact ggcgcaatgg
    12781 agcgagcagc cgattcaacg taggtcatat ctggctcatg atcgccttta aacggcgtag
    12841 gtacagcaat cagccaggca tccgcttcaa ctggcgtcgt gctcgctcgt aaaaaaccgc
    12901 cttctacggc agtttttact acgctcgcca aatcaggctc gacgatatgg atttcgccac
    12961 gattgatggt atcaaccgcg tgttggttga tatcgacacc aatcacctgt ttttgccgtg
    13021 aggcaaacgc tgctgctgta ggcagcccga tgtaacccag tccgataaca gaaatggtcg
    13081 caaaactcat agtgatatcc gattattttt taacgcttcc agaatgcgag agcatgcctg
    13141 accatcacca tacgggttat gggcgcggct catagcttga tattcgtttt cgtcttttaa
    13201 aagacgcgtc acttcctcga caattcgctg cttatccgtg cctaccagac gcaccgtacc
    13261 cgccgtcacc gcttccggac gctcagtggt atcgcgcatc accagcaccg gtttccccag
    13321 cgaaggcgct tcttcctgaa tgccgcctga gtcagtcaaa atcagccagg cgtggttcat
    13381 cagccagaca aacggtaaat actcctgggg atcgatcaga atgacatttt tcacatgccc
    13441 cagaatgcga ttgaccggct ctctgacgtt cgggttgaga tgcaccggat agacaatctg
    13501 gatgtcctgg tgcgtggtgg cgatgtctgc cagcgcgtgg cagatttctt caaagccacg
    13561 accgaaactc tcacgcctat gaccggtcac cagaatcatc tttttatcgg gggcgataaa
    13621 cgggtaattt gccgccagtt ctgaacgcag cgtgtcgctg ctcatcacct ggtcacgcac
    13681 ccataacagt gcatcaatga ctgtattacc ggtaatgaag attcggctat ccgcaacgtt
    13741 ttcacgcagc aagttttgcc gggaagtttc ggttggagag aagtgataca tcgccagatg
    13801 cccggtcaat gtacggttag cctcttccgg ccacggcgaa tagagagcgc ccgtgcgcag
    13861 accagcctca acgtgaccaa caggaatacg ctgataaaac gccgccaggc tggttgccag
    13921 cgtcgtcgtc gtatcgccgt gaaccagcac gacgtctggt ttgaactcgg caagaatagg
    13981 ttttagccct tccagaatcc gacaggttat ctctgtcagg ccctgtcctg gctgcattat
    14041 gttgagatcg tagtcaggta caatggaaaa gagtttcagt acctgatcaa gcatctcccg
    14101 atgctgcgca gtgacgcaaa ctttagcctc aaaaaaagga tcttttgcca acgcatgcac
    14161 caacggagcc atcttgatgg cttccgggcg cgtaccaaat acagtcagta ctttcacatc
    14221 gattctcttc gaataagcgg cgagcgcctt tgcgctcacc gcagcagtgt tgctatttcg
    14281 agcaacggcg ggttaatgcg acaccagccc cgatcagccc cccgacaatg ccccacataa
    14341 tcatcaggaa ggcacgacgt gggctatcgc gttttaccgg ttcttccggc gtacgcaaat
    14401 agcgataggt ctgaaaacgc ggatccaggg ttggaccaac attcagggtg tttaacatgg
    14461 ctcgattctg atcatagtcg agatcaaagg ccggaccgac ggcctgtaaa ttttccagtc
    14521 gagcctggag cattggacgc ccaagcagga acatttctga atcaggtaat tcctcggcag
    14581 gcacatctgt cgcactgcgc gaaatattat gctgctcagc aattttcagc gcctgctcaa
    14641 tactgttcat ccggcggtcg tagatggctt tcgccacctc ttcctgacgc ttcacctgag
    14701 ctttcatctg gatggtgcgt gccgcccatg cgcctttcag ctcatcattc agatggctgg
    14761 ctgcacgctg gctggcaaaa gcaacatact gacgtaacag gttattagcg tcaggcgcgg
    14821 tttcggcaat aagcttcacg ctgtcattga ccgcgcgagt aaagtctccg gggataaact
    14881 ggatgttgtt aatcatttca tccagcaacg ccgcatcggc tttgctgttg cccaccatcc
    14941 gctgtttgta atagtcggtt tgcagccaga actctctgcg ggtatcccac gaggccagtt
    15001 gcataacaaa ctctttatag gcttcgtcca tgaccgatgg ttgatcggca gaagccatgt
    15061 ttgaacggac atccaggtta cgcaaaaatt gctgctgcga gtaatatccc cccagcatat
    15121 tcaccgttgg acgatcggta atcgccgtcg agctccactc ctgacgagca aaaaaagtat
    15181 acgccagcgc gattaacgca aacgccagcc ccatgccaat aatccatagc ttcccagccc
    15241 acaaggtacg aaacaaccca cgaatatcca gttcattttc agcgtcttcg gccggtttcc
    15301 caggcattgg ttgtgtcatc acatcctcat ttatttggtt aaattggggc tgccaccacg
    15361 atttctacgc agtctgcgtt ttacgcgctt aataaagcga gcaactttcc aggcacgctt
    15421 aatgcaatat ccatagagga agaatgctag caaaaagagc accagcatga cccactccgg
    15481 gacaaaatga gaatattctg ccagcacgcc aatggaagcg agcagtgctg cggcaagggt
    15541 aatcagcaca aacgcctgac gggaagtaaa cccggcacgc atgatcaaat ggtgaatatg
    15601 ctgacggtca ggagagaatg ggctcatgcc tttacgcagg cgacggtaca taatcgccac
    15661 catatccatt agcggaatgg cgattatcca caaagcggta accgggctga tgggatgggt
    15721 tttgccctgg gtcgtttcga gcaggatcca gataacggta aaaccaatca gcgtactgcc
    15781 cgcatcaccc ataaagactt tgtagcggcg acccaggata ccaaggttaa gcatgatgta
    15841 tggcaggatg gcggcgatca tcgcaaagca ccagattgcg aggctggttt gcccgtcgaa
    15901 ccacaaaatc ataccgattg ctgcaaacga gacgcaggac aacccgccca gcaagccatc
    15961 aatgccatca accatgttga acgcattaat ggccgcccag acggcaaata gcgtcaggaa
    16021 gtaaccaaac ggtccgagca ccatctccca ggagccaaag atataaccca ggctactgag
    16081 ataaagtttg ccgaacacca tcataacaat gccaacagcg gcctgtatgg tggcacggat
    16141 ttttacgctg atatcaaaac gatcatccag cgcgccaatg aaaacaagca caccggcaca
    16201 agcgagatag agagatgcat gcggaatata gtaatcgaca attccgaacg tgaagcaaat
    16261 ccctgcgtaa accgaaatcc ccccaacgag aggtatcaat ccctggtgac gtttgcggaa
    16321 gtttggttta tccactaaac cgactttttt tgccacctta cgggcaaaaa acagaaacag
    16381 tgtcgtgaat aaaaaaatac tgatgagatc agtactcact gtcagtaaat tcacaatgca
    16441 tgctctcaga gaaaattatt agcagaagta taaccacgaa gacctttatt cagaagggaa
    16501 agtccgttcc gaacccacca gccctgtgca aaaatcaacc atttgatatg caaattgcat
    16561 aacatgccag caaattccag gaattttcct aacatcgcca gcgcgacatt aagattacag
    16621 atataaaagc aaaacgccac gtaaacacgt ggcgtttttg gcataagaca aatttatgag
    16681 cgtttcatca tttcgaagaa atcgtcattg gtcttggtca tggccagttt attaatgagg
    16741 aattccattg catcgatttc gcccatcggg tgaatgattt tgcgcaggat ccacattttc
    16801 tgcagttctt cctgagtcgt gagcagctct tctttacggg taccggaacg gttgtagtcg
    16861 atagccggga agacgcgttt ttcagcgatc ttacgagaga ggtgcagttc catgttgcct
    16921 gtacctttaa actcttcgta aataacttcg tccattttag aaccggtatc gataagcgcc
    16981 gtcgcgataa tggtcaggct gccgccctct tccacgttac gcgccgcacc aaagaagcgt
    17041 ttcggacgat gaagggcatt ggcgtccaca ccaccggtca acactttacc tgacgccgga
    17101 acaacagtgt tgtaagcgcg cgccagacga gtgatggagt cgagcagaat gataacgtct
    17161 ttcttgtgct caaccaggcg tttggccttc tcgatcacca tttccgcaac ctgaacgtgg
    17221 cgagatgcgg gttcgtcaaa ggtagaagca acaacttcac cttttaccag acgctgcatc
    17281 tcggttactt cttccggacg ttcgtcgatc agcagaacca tcagcacaca atccgggtgg
    17341 ttgtaagcaa tgctctgagc aatgttctgc agcagcatag ttttaccggc tttcggcggt
    17401 gccacaatca gaccacgctg accacgaccg ataggtgatg ccagatccag tacgcgagca
    17461 gttaaatctt cagtagaacc gttaccacgt tccatacgca gacgagagtt tgcgtgcagc
    17521 ggggttaagt tctcaaagag gattttgttg cgggcgtttt caggtttgtc gaagttaact
    17581 tcgttaactt tcagcagcgc aaaatagcgt tcaccttctt tcggcgggcg aatcttacca
    17641 gagatggtat caccagtgcg gaggttgaaa cggcggattt ggctagggga aacgtagatg
    17701 tcatcaggac cggcgaggta ggagctgtct gcggaacgga ggaaaccaaa tccatcctgc
    17761 aatatctcca gtacgccatc accaaagata tcttcgccac tctttgcgtg ctgcttcagg
    17821 atggcaaaaa taatgtcctg cttacgcatg cgagccaggt tttccagccc catattttcg
    17881 ccgagagtga tcagctcaga aaccggcgta ttctttaatt cggtaagatt catagtggtg
    17941 tgagttctta aacttggggt aaatctcgaa cttaatgttg tgaatggtat ggcaggatca
    18001 tccatgcctg tttacggtca tcgactcatg tcttttcgct gcctggtcac aggagagtac
    18061 gcagaactga aacgacaaga cggattgagt gacgagccag aaatctgttc acttcgcatt
    18121 taagataaaa cgggaagcgt tggaaattac aagattcaaa cttaataagg tatgtttaat
    18181 acgaagtcaa cactaagtta gcatgactca cgccgggcgt ccagttttta gcgacggggc
    18241 acccgaacat gaaattccct tacgccaggt tagcgtcgag gaactctttc aactgacctt
    18301 tagacagtgc gcccactttg gttgccgcca cttcaccgtt tttgaacagc agcagagtcg
    18361 ggataccacg gatgccatat ttcggcgcag tgccagggtt ttgatcgatg ttcagttttg
    18421 caacggtcag tttgccctga tattcgtcag cgatttcatc cagaatcggg gcgatcattt
    18481 tgcacggacc gcaccactct gcccagaaat cgacgaggat cgccccgtcc gctttgagta
    18541 catccgtgtc aaaactgtcg tcagtcaggt gaataatttt atcgctcata tataactcca
    18601 caggaataag cctggcgtgt tggtttcgtt gttggtgtaa cattaaccaa ctaaaggttg
    18661 actttatttc accggatacg ctttcgtaaa gcaatagtaa gctgatattc taccacacta
    18721 tgagcaaaac acatttaaca gaacagaagt tttccgactt cgccctgcat ccgaaggttg
    18781 tagaagccct tgaaaaaaaa gggtttcata actgtacgcc cattcaggca ctggcccttc
    18841 cgctgacgct ggcgggtcgt gacgtagccg ggcaggcgca aaccggtacc gggaaaacga
    18901 tggcgtttct tacgtcaacg tttcattatc ttctctctca tcctgcgatt gccgatcgca
    18961 aggtgaatca accgcgtgcc ttaattatgg caccgacgcg tgaacttgcc gtgcagatcc
    19021 acgccgacgc ggaaccgctg gcggaagcta ccggcctgaa gctgggcctg gcttacggtg
    19081 gtgatggcta tgacaaacag ctgaaagtgc tggaaagcgg cgttgacatt ctgattggca
    19141 ccacgggtcg tttaattgac tacgccaagc agaaccacat taacctcggt gccattcagg
    19201 tagtggtact ggacgaagcc gatcgcatgt acgatctagg ctttattaaa gatatccgct
    19261 ggctgttccg ccgtatgccg cctgcaaatc agcgcctcaa catgctgttc tccgccacgc
    19321 tttcgtaccg ggtacgtgaa ctggcgttcg agcagatgaa caatgccgaa tatattgaag
    19381 tggaaccgga acagaaaacg ggccaccgta ttaaagaaga gcttttctac ccttctaacg
    19441 aagaaaaaat gcgtttgctg caaacgctga tcgaagaaga gtggccagac agagcgatta
    19501 ttttcgccaa taccaaacac cgttgtgaag agatctgggg ccacctggcg gcagatggtc
    19561 atcgtgtcgg tttgttgaca ggcgatgtcg cgcagaaaaa acgtctgcgt attcttgatg
    19621 aatttacccg tggcgatctg gatattctgg ttgccaccga cgttgccgcg cgtggtttgc
    19681 atattccggc agtgacgcac gtctttaact acgatttacc cgatgactgt gaagattacg
    19741 ttcaccgtat tggtcgtaca ggtcgcgcag gcgcaagcgg tcactctatc agcctggcgt
    19801 gtgaagagta tgcattgaat ttgcctgcta ttgagaccta tattggtcac tcaattccgg
    19861 taagcaaata caatccggac gcattgatga ccgatctgcc aaaaccgctg cgcctcacgc
    19921 gcccgcgcac aggcaatggt ccgcgtcgta ctggcgctcc gcgtaatcgt cgtcgttcag
    19981 gttaaataaa atgtcatact caaaatcatt caaactgtat caagatggca agagaatgaa
    20041 tcccgatgag cttactgtag taagtgattc gggtgaaaga atgcagccaa cacagagaca
    20101 gattgaagga tgaagagtat gggttccacc tcgtcgctgt atgcagccat tgatctcggt
    20161 tcgaatagtt ttcatatgct ggttgtgcgc gaggtggctg gaagcatcca gacgctgacg
    20221 cgaattaaac gcaaagtgcg tctggctgct ggcctgaata gcgaaaatga cctctctaat
    20281 gaagcaatgg agcgcggttg gcaatgtctg cgcctgtttg ctgaacgtct gcaagatatc
    20341 cctccctcgc aaattcgcgt tgtcgctacg gcgacattac gacttgccgt caatgcgggt
    20401 gattttattg ccaaagcgca ggaaatcctc ggttgtccgg tacaggtgat cagcggtgaa
    20461 gaggaagcgc gtctgattta tcagggcgtt gctcacacca ctggtggtgc cgatcagcgc
    20521 ctggtggtgg atataggcgg tgccagtact gaactggtaa ccggcacggg tgcacaaacc
    20581 acctcgttgt tcagcctgtc gatgggctgc gtcacctggc tggaacgcta ttttgccgat
    20641 cgtaatctgg ggcaagaaaa ttttgatgct gcagaaaaag cggcacgcga agtgttacgt
    20701 ccggttgccg atgaattacg gtatcacggc tggaaagtgt gcgttggtgc ttccggcacc
    20761 gtgcaggcgt tacaggaaat catgatggca caggggatgg atgaacgcat taccctggaa
    20821 aagttgcagc aattgaaaca gcgagccatt cattgcggtc ggctggagga actggagatt
    20881 gacgggctga cgctggaacg tgcattagtg ttcccgagtg gtctggcgat cctgatcgcc
    20941 atttttaccg aactgaatat tcagtgtatg accctggcgg gcggtgcgct gcgtgaaggc
    21001 ctggtctacg gcatgttgca tctggccgtc gagcaggata ttcgcagccg tacactgcgt
    21061 aatattcaac gccgctttat gatcgatatt gatcaggcac agcgcgtagc caaagttgcg
    21121 gctaacttct tcgatcaggt ggaaaacgaa tggcatcttg aagcaataag ccgcgatttg
    21181 ctcatcagcg cctgtcagct tcatgaaatc ggcctgagcg ttgacttcaa acaagctccg
    21241 caacacgctg cttatctggt gcgtaacctg gatcttcccg gttttacccc cgcacagaag
    21301 aaactgctgg cgacgctact gctcaaccag actaacccgg tcgatctctc atcgctgcat
    21361 cagcaaaatg ccgtaccacc gcgcgtcgca gaacaactct gccgtttact ccgcctggct
    21421 atcatttttg ccagccgtcg ccgtgacgat ctcgtgccag agatgacatt acaggctaac
    21481 catgaactgt tgaccttgac gcttccgcaa ggttggctaa cccaacatcc gctgggtaaa
    21541 gagattattg ctcaggaaaa ccagtggcag agctatgtcc actggccgct ggaagtgcat
    21601 taatgagtaa gtgccggatg cgatgctgac gcatcttctc cggccttgat tatttccctc
    21661 gttttgccgc catcatcgct ttcagattcg ccagatggct ttgccctttc tgcatccgtt
    21721 cttcggcact gaccactttg cgctcctgtt cccaaatcag atcatcctgc ggcagctcca
    21781 gcaaaaagcg gctcggctcc gggcgcacca gttcgccgta ctgacggcgt tctttacaca
    21841 gcgtaaaggt caattccttc tgggcgcggg taatgccgac ataggccagc cgccgctcct
    21901 catcgatatt atcttcatcg atactgctct ggtgcggcaa aaacccttct tccataccga
    21961 ccatgtagac ataaggaaac tccagccctt ttgacgcgtg gagagtcatc agttgcacct
    22021 gatccagctc ttcttcactc tcaccacgct ccatcatgtc gcgtaaagta aagcgcgtca
    22081 ccacctgggt gagcgtcatc ggctcatcca gttcgctgcc ttccagcatc tccgtcatcc
    22141 agctaaacag ttggttgacg ttcttcatgc gcatttcggc agctttcgtg ctgggcgatg
    22201 tttcgtacag ccaggattca taatccatgc cgtggatcag atcgcgcacc gcggcaatcg
    22261 gctcccgctc cgccagacgc tggatttctg ccaaccagtg agtgaagcgg gtcaatgctt
    22321 cataaccacg tccgctaagc gtctgactca ggcccatatc aaagctggcg gtaaacatgc
    22381 ttttattgcg cgtcatcgcc cactcaccca gctttttcag cgtcgccggg ccaatctctc
    22441 gcttcggcgt gttaacgata cgcagaaatg cgctgtcatc gtccgggtta gtcagcacgc
    22501 gcagataagc cagcaagtcc ttgatttcag gacgagagaa aaacgacgta ccaccagaaa
    22561 ttttgtacgg gatgcggttt tgcatcagga atttttcaaa cacccgcgac tgatagttac
    22621 cgcgataaag aatagcgtaa tctttgtact gcgttttatt gacgaagtga tgcgcaatca
    22681 gttcaccagt gacgcgctcg gcttcatgtt cttcgttatt cgcgcttaaa acttttagct
    22741 ctgtgccgta acccagttcg gagaacagac gcttttcaaa gacgtgcggg ttattggcga
    22801 tcaggatgtt cgccgctttc aaaatacgtc cggaagagcg gtagttctgc tcaagcttaa
    22861 tcaccttcag cgccggaaaa tcctggctca gcagcaccag gttttgcgga cgtgcgccgc
    22921 gccaggagta gatcgactgg tcatcgtcac ccaccacggt aaagcgcgcg cggctgccca
    22981 ccagcagttt caccagttca tactggctgg tgttggtgtc ctgatattcg tccaccagca
    23041 ggtagcgaat tttgttctgc cagcgctcgc ggacttcttc attgcgttgc agaagtagcg
    23101 tcggcaataa aatcagatca tcgaagtcga gcacattaca ggctttcagg tgtgcatcat
    23161 acagcccata acaatgggca aaaatacggt cccgctcgcc aatcgcactg gctgccgcct
    23221 gggacggtgt tttgagatcg ttcttccagt tagagatggt cgaaatcaac tgttgcagga
    23281 gaactttgtc atcttcaatc agcccttcgg tcaactcttt gagcaacgcc agctgatcgg
    23341 tatcgtcaaa cagcgaaaag ttcgccttca tccccagcgc cgcatactca cgcttgatga
    23401 tatccagccc cagcgtgtgg aaggttgaga tcatcagccc atgcgcctct ttgcgaccca
    23461 gcgtctgtcc gacacgctct ttcatctcgc gcgccgcctt attggtaaag gtcaccgccg
    23521 cgatatgtcg cgcctgataa ccgcacccgc ggatcagatg ggcgatttta ttggtgataa
    23581 cacgagtttt accggaaccc gcacccgcca gcaccaggca ggggccggta acgaattcga
    23641 cagcttgttg ttggccgggg tttagacgca taggaaaatc gctcaatgaa agtcgggagg
    23701 ggaaaataac agcgtggtag tatagccagc ctcatcctta ccactcaagg cacgatcatg
    23761 gcaaaaacag cagcagcact gcatatcctt gtaaaagaag agaaactggc tctggatctt
    23821 ctcgtgcaga ttaagaacgg tgccgatttc ggcaagctgg cgaagaaaca ctccatttgc
    23881 ccatcaggca aacgcggcgg tgatttaggt gaattccgcc agggtcagat ggttccggcg
    23941 ttcgataaag ttgtgttctc ttgtccggta ctggagccaa ccggcccgct gcacacccag
    24001 ttcggttacc acatcattaa ggtgctgtac cgtaactaat agcaaggcct tctccgggag
    24061 aaggccttga actctgaatc gctattaacc cgcaacagca atacgtttca tatccgtcat
    24121 atagccgcgc agtttcttac ctacttgctc aatcgcatgg ctgcgaatcg cttcatttac
    24181 atcacgcagc tgcgcgttat ctaccgcacc ttccggaata gctttaccca aatcgcccgg
    24241 ttgcagttct gccataaacg gtttcagcag cggcacacaa gcgtaagaga acagatagtt
    24301 accgtactcg gcagtatcag agataaccac gttcatttca tacagacgct tacgggcgat
    24361 ggtgttggca atcagcggca gttcgtgcag tgattcatag taagcagatt cttcgatgat
    24421 gccggaatca accatggttt cgaacgccag ttcaacgccc gctttcacca tcgcgatcat
    24481 cagtacgcct ttatcgaagt actcctgctc gccgattttg ccttcatact gcggcgcggt
    24541 ttcgaaggcg gttttgccgg tctcttcacg ccaggtcagc agtttcttat catcgttggc
    24601 ccagtccgcc atcataccgg aggagaattc gccggagatg atgtcgtcca tatgtttctg
    24661 gaacagcggt gccatgatct ctttcagctg ttcagacaac gcgtaagcac gcagtttcgc
    24721 cgggttagag agacggtcca tcatcagtgt gataccgccc tgcttcagcg cttcggtgat
    24781 ggtttcccaa ccgaactgaa ttagtttttc tgcgtatgcc ggatcggtgc cctcttccac
    24841 cagcttgtcg aagcacagca gcgagccagc ctgcaacata ccgcacagga tggtttgctc
    24901 acccatcagg tcagatttca cttccgcaac gaaggacgat tccagcacgc ccgcacggtg
    24961 accaccggtt gctgccgccc atgctttagc gatcgccatg ccttcgcctt tcggatcgtt
    25021 ttccgggtga acggcaatca gcgtcggtac gccgaatcca cgtttgtact cttcacgcac
    25081 ttcggtgcca gggcatttcg gcgcaaccat gactacggtg atgtctttac ggatctgctc
    25141 gcccacttcg acgatgttga aaccgtgcga gtaacccagc gccgcgccgt ctttcatcag
    25201 tggctgtacg gtgcgcacta catcagagtg ctgcttgtcc ggcgtcaggt taaccaccag
    25261 atccgcctgt ggaatcagtt cttcgtaagt acccacttta aaaccatttt cggtcgcttt
    25321 acgccaggac gcgcgcttct cggcaatcgc ttctttacgc agagcgtagg agatatcgag
    25381 accagaatca cgcatgttca gaccctggtt cagaccttgt gcgccacagc cgacgatgac
    25441 tactttttta ccctgaaggt agctcgcgcc atcggcgaat tcatcgcgcc ccataaagcg
    25501 acatttgccc agctgtgcca gctgctgacg cagattcagt gtattgaagt agttagccat
    25561 ggtgattcct cgtgatgttg tgcttcttat tgttcggttc gcgtttgcga gattgaattc
    25621 actatatgac aggaaattta ttgcggaaat tgatatattc acaacgtcac attgcaattt
    25681 ctgcaacgtc aacattgagg gctgcgccat tggatttacg cgatctgaaa accttcctgc
    25741 atctggcgga aagccgccat tttggccgca gcgcgcgggc gatgcacgtt agcccgtcca
    25801 cgctctcacg gcagattcag cgcctggaag aagatctcgg ccagccgctg tttgtgcgcg
    25861 ataaccgcac ggtgacgctg actgaagcgg gcgaagaact gcgcgttttc gcccagcaaa
    25921 cgctgttgca gtatcagcag ctgcgccaca ccatcgacca gcaagggccg ttgctctatg
    25981 gcgaattaca tatcttctgc tcggtgaccg ccgcttacag ccatctgccg ccgattctgg
    26041 atcgcttccg cgcggaacac ccgtcggtgg agatcaaact cactaccggt gatgcggcag
    26101 atgcaatgga aaaagtcgtc acaggtgaag ctgatctggc gattgccggg aagccagaga
    26161 ctctgcccgg cgcggtggcg ttttcgatgt tggagaatct ggcagtggtg ttgattgcac
    26221 ccgcattgcc ctgcccggtg cgtaatcagg tttctgtcga aaagccggac tggtcaacgg
    26281 taccgtttat catggccgat caggggccgg tgcgccgccg cattgagttg tggttccgac
    26341 gcaataaaat cagtaacccg atgatttacg ccacggttgg cgggcatgaa gcgatggtat
    26401 cgatggtggc gctcggctgt ggcgtggcat tgttgccgga agtggtgctg gaaaacagcc
    26461 ccgaaccggt gcgtaaccgc gtgatgattt tagagcgcag cgatgagaaa acgccgtttg
    26521 agctgggcgt gtgtgcgcag aaaaagcggc tacatgagcc gctaattgag gcattctgga
    26581 agattttgcc gaaccattaa cccgccaaaa agaacctgaa cgccgggtta ttggtttcgt
    26641 cgtggcaatc gtagcccagc tcattcagcc gggtttcgaa atccggttca tggtcgccaa
    26701 gttcgaacgc cgccagtacg cgcccgtagt cggtgccatg gctgcgatag tggaacaaag
    26761 aaatgttcca gtgcgtaccc agcgtgttga ggaagcgcag cagcgcgccc ggtgattccg
    26821 ggaattcgaa gctgtagagg cgttcctgca acggatgtga cggacgcccg ccgaccatat
    26881 agcgcacatg caacttcgcc atttcgtcgt cggagagatc caccacgctg tagccgccgt
    26941 cgttgagcat ctgcaaaatt tctttgcgct cttcgaggcc gcggctcagg cgcacgccga
    27001 caaagatgca ggcgtttttg gcatcggcaa aacggtagtt gaactcggtg accgatcgcc
    27061 cgccaagcag ttggcagaat ttgaggaagc tgcctttttc ttccggaatg gtcaccgcca
    27121 acaacgcttc acgctgttcg cccagttcgc agcgttccga gacgtagcgc aggccgtgga
    27181 agttcacgtt ggcaccggaa agaatatgcg ccagccgttc gccgcgaatg ttgtgctggg
    27241 cgatatattt tttcattccc gccagcgcca gcgcgccaga gggttccgcc accgcacgca
    27301 catcttcgaa taaatccttc atcgccgcac agatcgcatc gctatcgacg gtgatgatgt
    27361 cgtcgagata ctcctggcat aaacggaagg tttcgtcacc gatgcgtttt accgcgacac
    27421 cttcggcgaa cagtcccacg cgtggcagat caaccggatg acccgcatcc agcgctgctt
    27481 tcaggcaggc ggagtcttcc gcttctacgg cgatcacttt gatttgcggc atcagttgtt
    27541 tgatcagcac cgctacgccc gccgccagac cgccgccgcc gactggcaca aatacgcggt
    27601 cgagatgggc gtcctgctgg agcagttcca gcgccagcgt gccttgcccg gcaatcacca
    27661 tcggatggtc gaacggcggc acccaggtga acccctgctg ctgtgacagt tcgatcgctt
    27721 tagctttcgc ttcgtcaaag ttcgcgccat gaagcagcac ttcgccgccg aatccacgta
    27781 ccgcatcgac tttgatgtcg gcggtggcgg ttggcataac gatcagggcc ttcacgccta
    27841 accgcgcaga agaaaacgcg acgccctgcg cgtggttacc cgcagaagca gtgatcacgc
    27901 cgtgcgcttt ttgttcttcc gtcaggcccg ccatcatggc gtatgcgccg cgcagcttaa
    27961 agctgtgcac tggctggcga tcttcgcgct tcaccagaat gacgttatca agacgcgacg
    28021 acagtttttc cattttttgt agcggcgtaa cctgcgccgc ctcgtaaacc ggcgcgcgca
    28081 gcactgctct taaatattcg gcaccttccg gagcaccgga caggggttgc gagtcagcca
    28141 ttattaaccc cccagtttcg atttatcgcg caccgcacct ttgtcggcgc tggttgccag
    28201 gctggcgtag gcgcacaggg caaaggaaac ctgacgttca cggtttttcg gcgtccaggc
    28261 tttgtcaccc cgagcttcct gcgcttcacg acgcgccgcc agttcggcat cgcttacctg
    28321 taactgaatg ccacggttcg ggatgtcgat agcgatcagg tcaccatctt caatcagacc
    28381 aatgctgccg ccgcttgccg cttccggtga gacgtggccg atggaaagac cagaggtgcc
    28441 gccagagaaa cgaccgtcgg tgatcagcgc acaggcttta ccgagaccca ttgatttcag
    28501 gaagctggtt gggtagagca tttcctgcat gccagggccg cctttcgggc cttcatagcg
    28561 gatcaccacc acatcgcccg cgacaacttt gccaccgagg atcgcttcta ccgcgtcgtc
    28621 ctggctttcg tacactttcg ccgggccggt gaatttgagg atgctgtcat cgacgcccgc
    28681 ggttttcacg atgcagccgt tttcagcaaa attaccgtag agcaccgcca ggccgccgtc
    28741 tttgctgtag gcgtgttcca gcgagcggat acagccgttt gcgcgatcgt catcgagaga
    28801 atcccaacgg caatcctgcg agaatgcctg tgtagtacga atgcccgcag ggcctgcgcg
    28861 gaacatattt tttaccgcgt catcctgggt cagcataacg tcgtattgtt ccagagtttg
    28921 cggcaacgtc aggccaagta cgtttttcac atcacggttc agtaaccccg cgcgatccag
    28981 ttcaccgaga ataccgataa caccaccagc acggtgaaca tcttccatat ggtatttttg
    29041 ggtgctcggc gcaactttgc ataactgcgg gactttacgc gaaagtttgt cgatatcact
    29101 catggtgaag tcgatttccg cttcctgcgc cgccgccagc aggtgaagta ccgtgttggt
    29161 agagccgccc atcgcgatat ccagcgtcat ggcattttca aacgccgctt tgctagcaat
    29221 attgcgcggc agtgcacttt cgtcgttttg ctcgtagtaa cgtttggtca attcaacaat
    29281 gcgtttaccg gcattgagga acaactgctt acggtccgcg tgggttgcca gcagcgagcc
    29341 gttgcccggc tgcgacaggc ccagcgcttc ggtcaggcag ttcattgagt tagcggtaaa
    29401 catcccggag caggagccgc aggtcggaca cgcggaacgt tcaacctgat cgctctggga
    29461 gtcagaaact ttcgggtctg cgccctggat catcgcatca accagatcga gtttgattat
    29521 ctgatcggac agtttggttt tcccggcctc catcgggccg ccggaaacaa agatcaccgg
    29581 aatattcagg cgcagggaag ccatcagcat ccccggggtg attttgtcgc agttagagat
    29641 gcagaccatg gcgtcggcgc agtgggcgtt gaccatatac tcaacggaat cagcgatcag
    29701 ttcgcgagat ggaagtgaat aaagcatccc cccgtggccc atggcaatcc catcatccac
    29761 cgcaatggtg ttgaactctt tggcaacgcc gccagccgct tcaatttgtt cggcgaccag
    29821 tttaccgaga tcgcgcagat ggacgtgacc cggtacaaat tgggtgaacg agttcacaac
    29881 cgcgataatc ggcttgccaa aatcggcgtc ggtcattccg gtggcgcgcc acagcgcacg
    29941 agcacccgcc atattacgac catgagtggt ggtggcggaa cggtacttag gcatacttta
    30001 tttactccca gtgtctgtct cgtaaatggg acggtgcgtg ccgtcccatt ttttgtattt
    30061 attgattaac ttgatctaac cagccccatt tatcttcggt ttcgccagtg aagaggccga
    30121 agaaggcttg ctgaatgcgt ttggtaaccg ggccacaacg gccttcgcca atctgaatac
    30181 cgtctacgct gcgcactggc gtgatttctg ccgccgtacc ggacataaag acttcatccg
    30241 ccaagtacag ggattcgcgc gacagcacct gctcacgcac ttcaattccc agctctttcg
    30301 ccagtttgat gatggcatca cgggtaatac ccggcagcgc ggaggaggtg aacggtgggg
    30361 tgaacagcac accatctttc acttcaaaca ggttttcgcc tgcgccttca gagatataac
    30421 cgttcacatc cagcgcgata ccttcctgat aaccgtggcg gcgcgcttcg ctacccacca
    30481 gcagggaaga gagatagtta ccaccggctt ttgccgcagt tgggatggtg tttggtgctg
    30541 cgcggttcca ggaggaaacc atcgcatcga tcccctgctc cagcgcttct gcgcccagat
    30601 acgctcccca cgggaaagcg gcgataatca catcggttga gtatcccgct ggcgggttta
    30661 ctcccatgcc aacattaccg acgaagatca gcggacggat ataggcgctg gtgagattgt
    30721 ttttgcggat cacgtcacga caagcttcca tcagctcatc aatgctctgt gaaaccggga
    30781 agcgatagat tttggcggag tcatgcagac gctgcatatg ctcacgatgg cggaatacaa
    30841 ccggtccttt gtgtgagtcg tagcaacgga tgccttcaaa aaccgaggtg ccatagtgca
    30901 gcgcgtgcga catcacatgc accttcgcgt cttcccagcg aaccatctcc ccattgaacc
    30961 aaatgtaatc agctttcttc gtggtcattt ttatattcct tttgcgctca ggcgcggatt
    31021 tgttgtgatg tggttgtgct ctggcagatg gcaacgtgtg cgacgtccac cagtttattt
    31081 aactgactaa acagtaagtc gaccgaccgt gggctggcaa cggtcaattc gatatttata
    31141 ttttgtgcat cgctggcggc agccatattc attgagcaga cgtggaaacc acgatgacgc
    31201 accacgcgta aaacacgttc taaggtttcc ggattgaagc gagccgatac attgacctga
    31261 tgttgcatca tgataatttc tccaacattt ctgaattact ggcacctggc ggcaccagcg
    31321 gccagacgtt ctcaagttcg tcgattgaga catgaagcag gtatggccca tcactgttca
    31381 gcatggtgtc gagtgccgct tcaacctggt ctttacgggt gatgtgttgg ccagggatgc
    31441 cgaaggcgct ggctaacatg aggaaatcgg ggttatcggt aagggtggtt tcgctgtatc
    31501 gttcctgaaa aaacagttgc tgccattgtc gaaccatccc taaccgttgg ttatcgagta
    31561 agacgatttt caacggtaac tgcttgcgtt ttacggtgcc cagctcttgc acattcatca
    31621 tgaaagagcc gtcaccggag atacagacaa cggtatcgtt cggtcgcgcg acttgtgcgc
    31681 caactgccgc cggtaaacca aaacccatgg tacctaagcc gctggaggtg atgaaatttt
    31741 ccgggcgagt gtgggcgatg tgctgcgcag cccacatctg gtgctgcccc acatctgtgg
    31801 tcacgacgca atccgcaggt ttacgatccg acagttgttt taacaacaac ggagcgtaga
    31861 tagcgtcacc gggatggtcg taacgccagg aatgttcatc acgcagctgc gcgcagtgtt
    31921 gctgccagtc attgatattt aacggctgct gtaatgctgg taacagagca tttaaatcac
    31981 cttgtaatgc cacatgtgcc tgacgcagct tgttcatttc tgccgggtcg atatccatat
    32041 ggataacact ggcgtgtggt gcgaaggtgt tcagtttgcc ggtcacccgg tcatcaaaac
    32101 gtgcacccac ggcgatcagc aggtcacact cctgcaccgc gaagtttgcc gctttggttc
    32161 cgtgcatccc cagcatgccc agatagtacg gataatctgc ttctactgcg cccagccctt
    32221 tcagcgtaca ggtggcaggc atttttgtgg cagcgagaaa ttcacgcaaa gccggaactg
    32281 cctgcgccat ccccacgcca ccgccaacgt acagcatcgg tttttgcgct tttgccagca
    32341 tctggcgcgc ttgctcgact tcggcatgtg ggaaagtcac ttcgttttca acggtggtga
    32401 accacggttc cagatcgccg ctggctaact ggatatcttt tgggatatcg accagaaccg
    32461 gaccaggacg acctgagctg gcaacgtcga atgcttcagc catgatgcgc ggcaactctt
    32521 ccagagactg caccagaaag ctgtgcttgg tacaggctaa cgacaatccc aggacatcca
    32581 cttcctgaaa tgcgtcggtg ccgataaacg gtgcggacac ctgaccagtg atggcaacga
    32641 cagggatgga atctaacagt gcgtccgcaa gcccggttat caggttggtt gcgcccggac
    32701 cagacgtggc gatacatacg ccagttttac cggtagcacg ggcataaccg atagccgcca
    32761 ttgccgcacc ctgctcatgt cggcacagca agtgctccac gccgccgtca tacaatgcat
    32821 cgtaaaccgg cataattgcg ccacccggat aaccgaaaac ggtgttcaca ccctgtgccc
    32881 gcaacgcatg taccacccac tgtgcgccat tcatagttag ttccccgtcc tgaatcttga
    32941 gaaacagaat tttgtgctgt tattcattgt ctgctcctcg gttatgtttt taaggtcaaa
    33001 aaaacccccg gacctttcgg tgcgggggtc ttagttcgtt aaggcttgat ctctaagcct
    33061 ttcctcgtcc aagtgcagcc ccgcacggtg ggataataat caccaccacg ctaatcacga
    33121 ccaggctaat cactcgtaga agggctgtca ttttgtcttt tcttgcatct tgttcgaagg
    33181 aatgcctaaa gagttaccat agattttgcg aatagcacaa gatatttttg ggcctatttt
    33241 tatgatggct ttaataattt caatagaaat tgttgttttt taaaaagaaa atagtgagtg
    33301 aaaaaattca atttcagtat aaatgttgtt cccttcctcg tagttcatcc tttgtccgct
    33361 gcatttttgc gagcatcatt ccaccgccag aacaactcaa cttcacttgc acaaataaac
    33421 aggaatcagc cactaaaatt cacgaattag cgggctacag cagcgatatc ttatgcacat
    33481 aatggtgact caaggagggt ttatgtcact gtcaattgtt catacccgcg cagccctgga
    33541 agtaaatgcg cccccaatca ctgttgaggt acatatcagt aaaggtctac ccggcttaac
    33601 gatggtgggc ttaacagaaa caacggtgaa agaagctcgc gatcgcgtgc gcagcgccat
    33661 tatcaatagc ggatatgaat atccggcgaa aaaaatcacc atcaaccttg ctccagccga
    33721 tctgccaaaa gaagggggac gatatgattt acctatcgcc attgcgttgc tggcggcctc
    33781 agaacagctt acagccaata agttagatga atatgaatta gtcggagaac tagcgcttac
    33841 aggcgctctg cgtggcgttc ccggtgcaat ctccagtgca actgaagcta ttaagtcggg
    33901 cagaaaaatt atcgtcgcga aagataacga agatgaagtg gggctaatta acggtgaagg
    33961 atgcctgata gccgatcatc tgcaggctgt ctgtgcgttt ctggaaggta agaacactct
    34021 cgaacgcccg aaaccaactg atgcagtatc ccgggcgcta caacatgatc tcagtgatgt
    34081 tgtcggtcag gagcaaggaa aacgaggact ggaaattacc gccgctggtg ggcacaacct
    34141 tttactgatt gggccgccgg gaacaggtaa aacaatgctc gccagccgta ttaatggcct
    34201 gttgccagat ttaagcaatg aagaggcact ggagagcgct gcgatattaa gtctggtaaa
    34261 tgctgaatca gtacaaaaac aatggcggca gcgcccgttc cgctcacctc atcacagtgc
    34321 gtcgttaact gcgatggtgg gcggtggtgc aattccaggg ccaggtgaaa tttcgttggc
    34381 gcataacggc gtgctttttc ttgatgagct acctgaattt gaacggcgta cactggatgc
    34441 cttgcgagag ccgattgaat ccgggcagat ccatctttca cgcacacgag caaaaataac
    34501 ctatccagcc cgtttccagc ttgttgcggc gatgaatccc agccctaccg gacattatca
    34561 gggaaaccat aaccgctgca cgccagaaca gacattacgt tatctcaacc ggctctcggg
    34621 gccctttctc gaccgcttcg atctctcact ggagatccca ttaccacccc ccggcatttt
    34681 gagtaaaacg gtagtgccgg gagaaagcag cgccaccgtt aaacaacgcg taatggccgc
    34741 cagagagcgc caatttaagc ggcagaataa actgaatgcc tggctggata gtccggaaat
    34801 acgccaattc tgcaaacttg agagcgaaga tgcgatgtgg ttggaagaaa cgctgatcca
    34861 tctggggtta tcgattcgtg cctggcagcg gttactgaaa gttgcacgaa ccattgctga
    34921 tattgatcag tctgacatta tcacacgtca gcatttgcag gaggcagtta gctatcgtgc
    34981 gattgaccgt ttgctcatcc atctgcaaaa actactgaca taaaaaaagg gcatttcgcc
    35041 ctttttatta atcgtcagaa tcggtgtagt cttcagcacc ttcaacctgc ggtttaccgc
    35101 cggaaagggt gtgaaaacgt tttggacgct taatacgcgt catatacttg gaccacacgc
    35161 gttctgcttc tgtcactggc tcacgttcgc cacggcatac tgctacgaag agtttctctt
    35221 cctcggtaac cggctcgcgt ttgccaagat ccaactcatt gaaggcataa ccatgacgct
    35281 caagcagttg tgcctctttg atggtgaaat caccatgacg agagaatcca cgtggataat
    35341 gtttattgtc gaaatatcga ttagtcgtcg taaagctttc cgccatcctg cacgctccta
    35401 attctttgac cgagctagtt atggcgcgga gtattagtta cgcttgacag agtgtaaaac
    35461 aaaacattta aatcataacg acaaataatt ttgcggagag cactgtggat acggaattgt
    35521 taaaaacttt cctggaagtt agccgaacgc gtcactttgg tcgagcggct gaatcgctct
    35581 atctgaccca gtcagcagtg agctttcgaa tcagacaact ggaaaatcaa ctgggtgtga
    35641 accttttcac ccgccacaga aacaatatcc gtttaaccgc tgccggtgaa aaactactgc
    35701 cttatgcaga aacgctcatg agcacctggc aggccgcccg caaggaggtg gcgcatacct
    35761 cacgacataa cgagttttct atcggtgcca gcgcctcgtt gtgggaatgt atgcttaatc
    35821 agtggttggg acgcttgtat caaaatcagg atgcccatac aggcttacag ttcgaagcgc
    35881 gaattgccca acggcagtct ctggtaaaac agctgcatga acgccagctt gatcttctta
    35941 ttaccactga agcgcccaaa atggacgaat ttagtagtca gttgctgggg tatttcactt
    36001 tagcgcttta taccagtgcc ccttctaaac taaagggaga tcttaactat ctgcgactgg
    36061 agtgggggcc agattttcaa cagcatgagg caggtttgat cggtgctgac gaagttccca
    36121 ttctgacaac cagttctgct gaactggcac agcaacagat tgcgatgctt aatggttgca
    36181 cctggctacc cgtcagctgg gcgcgtaaaa aaggcggcct gcataccgtt gtcgatagca
    36241 caacactttc acggccgctt tatgccatat ggctgcaaaa tagcgataaa aatgcgttga
    36301 ttcgcgatct tttgaaaatt aacgtgctgg atgaagtgta ttaatctgaa tggctggcaa
    36361 ggatgccggt agaaggattt acttcggaga ggattatttc agataaaaaa aatccttagc
    36421 tttcgctaag gatgatttct ggcaggggcg gagagactcg aactcccaac acccggtttt
    36481 ggagaccggt gctctaccaa ttgaactacg cccctaatta gggtggcgga acggacggga
    36541 ctcgaacccg cgaccccctg cgtgacaggc aggtattcta accgactgaa ctaccgctcc
    36601 accgaattct tttacaacca ccggttttat gaccggctta c
//
LOCUS       DAJTQY010000031        34999 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.031, whole genome shotgun sequence.
ACCESSION   DAJTQY010000031 DAJTQY010000000
VERSION     DAJTQY010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34999
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.031"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(<1..815)
                     /locus_tag="OO349_001640"
     CDS             complement(<1..815)
                     /locus_tag="OO349_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR5866757.1"
                     /translation="MKPAHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRL
                     PELPQRLQSLYIPNNQLNTLPDSIMNLHIYADVNIYNNPLSTRTLQALQRLTSS"
     gene            907..993
                     /locus_tag="OO349_001641"
                     /pseudo
     CDS             907..993
                     /locus_tag="OO349_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1398..1874)
                     /locus_tag="OO349_001642"
     CDS             complement(1398..1874)
                     /locus_tag="OO349_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HCR5866758.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(1933..3222)
                     /gene="bioA"
                     /locus_tag="OO349_001643"
     CDS             complement(1933..3222)
                     /gene="bioA"
                     /locus_tag="OO349_001643"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HCR5866759.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKTQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAIIESGEWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            3309..4349
                     /gene="bioB"
                     /locus_tag="OO349_001644"
     CDS             3309..4349
                     /gene="bioB"
                     /locus_tag="OO349_001644"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HCR5866760.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFEPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLGQALMT
                     PDTDEYYNAAAL"
     gene            4346..5500
                     /gene="bioF"
                     /locus_tag="OO349_001645"
     CDS             4346..5500
                     /gene="bioF"
                     /locus_tag="OO349_001645"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HCR5866761.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQGAEQFGIGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            5487..6242
                     /gene="bioC"
                     /locus_tag="OO349_001646"
     CDS             5487..6242
                     /gene="bioC"
                     /locus_tag="OO349_001646"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HCR5866762.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADALLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLAQARHKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQARQAVDERP
                     HANRFLPPDEIEESLNGVRYQHHIQLITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            6235..6912
                     /gene="bioD"
                     /locus_tag="OO349_001647"
     CDS             6235..6912
                     /gene="bioD"
                     /locus_tag="OO349_001647"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCR5866763.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            7491..9512
                     /gene="uvrB"
                     /locus_tag="OO349_001648"
     CDS             7491..9512
                     /gene="uvrB"
                     /locus_tag="OO349_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HCR5866764.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVIVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMDELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLAEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDRSTPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMLPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(9704..10612)
                     /gene="yvcK"
                     /locus_tag="OO349_001649"
     CDS             complement(9704..10612)
                     /gene="yvcK"
                     /locus_tag="OO349_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HCR5866765.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPNVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     regulatory      10877..11021
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            11009..11998
                     /gene="moaA"
                     /locus_tag="OO349_001650"
     CDS             11009..11998
                     /gene="moaA"
                     /locus_tag="OO349_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HCR5866766.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEGDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     gene            12020..12532
                     /gene="moaB"
                     /locus_tag="OO349_001651"
     CDS             12020..12532
                     /gene="moaB"
                     /locus_tag="OO349_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HCR5866767.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDEAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            12535..13020
                     /gene="moaC"
                     /locus_tag="OO349_001652"
     CDS             12535..13020
                     /gene="moaC"
                     /locus_tag="OO349_001652"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HCR5866768.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            13013..13258
                     /gene="moaD"
                     /locus_tag="OO349_001653"
     CDS             13013..13258
                     /gene="moaD"
                     /locus_tag="OO349_001653"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HCR5866769.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADYPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            13260..13712
                     /gene="moaE"
                     /locus_tag="OO349_001654"
     CDS             13260..13712
                     /gene="moaE"
                     /locus_tag="OO349_001654"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HCR5866770.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDLQAAKRW"
     gene            13849..14553
                     /locus_tag="OO349_001655"
     CDS             13849..14553
                     /locus_tag="OO349_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCR5866771.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            14758..15471
                     /locus_tag="OO349_001656"
     CDS             14758..15471
                     /locus_tag="OO349_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCR5866772.1"
                     /translation="MESYSQNSNKLDFQHEARILNGIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPPMYVAIGLLLLCMYGLSKDINKINAAIAGVIYLFLLSLVAIVVASLVP
                     VYAIIIVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIVNAALMSERP
                     IWVISCLMIVLWPGIISHGRNKLLEQAGKCHSEELWSPVRCAFTGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(15507..16463)
                     /locus_tag="OO349_001657"
     CDS             complement(15507..16463)
                     /locus_tag="OO349_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HCR5866773.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRISSLSITTNWLGYILLAGIIFTAGVVELPDYWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(16463..17704)
                     /gene="clsB"
                     /locus_tag="OO349_001658"
     CDS             complement(16463..17704)
                     /gene="clsB"
                     /locus_tag="OO349_001658"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HCR5866774.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     AQREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGIQVFEYRRRPLHGKVALMVDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLTQVDP
                     PAQPTMETQDRVETENTGVNP"
     gene            complement(17701..18462)
                     /locus_tag="OO349_001659"
     CDS             complement(17701..18462)
                     /locus_tag="OO349_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCR5866775.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            18595..19005
                     /locus_tag="OO349_001660"
     CDS             18595..19005
                     /locus_tag="OO349_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HCR5866776.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(18967..20073)
                     /locus_tag="OO349_001661"
     CDS             complement(18967..20073)
                     /locus_tag="OO349_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5866777.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(20084..21217)
                     /locus_tag="OO349_001662"
     CDS             complement(20084..21217)
                     /locus_tag="OO349_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5866778.1"
                     /translation="MSNPILSWHRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQSGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(21210..22946)
                     /locus_tag="OO349_001663"
     CDS             complement(21210..22946)
                     /locus_tag="OO349_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5866779.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYHGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(22939..23934)
                     /gene="hlyD"
                     /locus_tag="OO349_001664"
     CDS             complement(22939..23934)
                     /gene="hlyD"
                     /locus_tag="OO349_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HCR5866780.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYNLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            complement(23937..24608)
                     /gene="cecR"
                     /locus_tag="OO349_001665"
     CDS             complement(23937..24608)
                     /gene="cecR"
                     /locus_tag="OO349_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HCR5866781.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            24837..26024
                     /gene="rhlE"
                     /locus_tag="OO349_001666"
                     /pseudo
     CDS             24837..26024
                     /gene="rhlE"
                     /locus_tag="OO349_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308902.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
     gene            complement(26231..26713)
                     /gene="ybiA"
                     /locus_tag="OO349_001667"
     CDS             complement(26231..26713)
                     /gene="ybiA"
                     /locus_tag="OO349_001667"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HCR5866782.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAARPIKVDGNTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRNRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRVLLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            26833..28983
                     /gene="dinG"
                     /locus_tag="OO349_001668"
     CDS             26833..28983
                     /gene="dinG"
                     /locus_tag="OO349_001668"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HCR5866783.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVTRREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDELLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEVQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFREQVESKKYLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            29011..29973
                     /gene="ybiB"
                     /locus_tag="OO349_001669"
     CDS             29011..29973
                     /gene="ybiB"
                     /locus_tag="OO349_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HCR5866784.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALCLSSVSHPEYIGRVAKFFSDI
                     GGWALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDPAGSELLPQAKDPETTAQ
                     WTERCLAGSEAIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            30114..31199
                     /gene="hcxB"
                     /locus_tag="OO349_001670"
     CDS             30114..31199
                     /gene="hcxB"
                     /locus_tag="OO349_001670"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HCR5866785.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCSAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMSEETLQAFCQQLAS"
     gene            complement(31428..31688)
                     /locus_tag="OO349_001671"
     CDS             complement(31428..31688)
                     /locus_tag="OO349_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR5866786.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(31953..32219)
                     /gene="ybiI"
                     /locus_tag="OO349_001672"
     CDS             complement(31953..32219)
                     /gene="ybiI"
                     /locus_tag="OO349_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HCR5866787.1"
                     /translation="MASAWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDRQLR"
     gene            complement(32293..32970)
                     /gene="ybiX"
                     /locus_tag="OO349_001673"
     CDS             complement(32293..32970)
                     /gene="ybiX"
                     /locus_tag="OO349_001673"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HCR5866788.1"
                     /translation="MMYHIPGVLSPQDVAHFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSALYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDKNIQSLKSRYGENEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(33012..34999)
                     /locus_tag="OO349_001674"
                     /pseudo
     CDS             complement(33012..34999)
                     /locus_tag="OO349_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
BASE COUNT         8296 a         8974 c         9457 g         8272 t
ORIGIN      
        1 gaagaggtta atctttgcag ggcttgcaga gtgcgagtcg acaatggatt gttataaata
       61 ttaacatctg cataaatgtg cagattcatg atactgtctg gcaatgtgtt cagctgattg
      121 ttagggatat atagtgattg cagacgttgt ggaagttcgg gcagtctgtg tagttggttt
      181 tccccaacgt ttaaaagcct cagattttga ggaaggggcg gtagctcttg taatctgtta
      241 tcaaaagcac ttagttcctg tagttcacat ggtaattctg gtaaggcagt aagctggttg
      301 ttgccaacag aaagtttttg tagagccggg ggaagatcag gtagcgtctc caggctattg
      361 agaagggctg agagtgactg caaggaagat ggaagagaag gcaaacatgt taaaactctg
      421 ttaagtgaaa catcaagagc gaccaggtga ggcggaagag ccgggagcct gctcagtctg
      481 ttatcgctgg ctttaagtac agttaaggac ggagggagtt ctggcagaga gcgtagctca
      541 ttgctagaga tgttaagttc ttgtatgtgc gggggcaggt atgggagaga gcgtaatctg
      601 agtaaactta aattgagggc tggctcttga aaagccagac atattttcag taatcgaact
      661 gcctgtgttc ggtcttctgt tgcagcacct tccttggccc agttatccca gatgcggtga
      721 taatccgtga tattttgctc ttctacggat aaacgggata tacatcctaa accacaaaag
      781 gagcggaaaa aagaaggatt gtgggcaggt ttcatttatt attgatcctc attgtttata
      841 tattaatata ttcgtgcaaa tggagtgagg attctttaaa aaaacgcgcc tgtaaatagt
      901 taagaacagg aagaactgtt ggggatgtgg ctggccgaaa atgaaggggt gaagttctgg
      961 ctcaatgtgc tgactgaact gaaaaaacgc ggttggtcgc cctacctttt catcaagcca
     1021 gtagtctgcc caccacagca tcatttctct gcgcttatcg agatactgag catggttgta
     1081 aattcagcta acccagtgcg ttgacaaaaa attagcgcaa gaggacaaaa aatcaccttg
     1141 cgctaatgct ctgtatcagg tcactaatac catctaagtg gttgattcat agtgactgga
     1201 tatgttgtgt tttgcagtat tatgcagtct attttttaag ctaaatgtat tataatgcat
     1261 tgatattaat gatttttact gtttctcggt cagctttttt atactaactt gagcgaaacg
     1321 ggaaggtaaa aagacaaaaa gttgttttta atacctttaa gtgataccag atggcattgc
     1381 gccatctggc agagtgatta actaaacatc gcggtaatcg atgcgcttgc cagagagtgg
     1441 aaatgaacgt taaacccgac catcgcgccg ctggctcctt catcgacatc aatacgctct
     1501 acatccagcg cgtgaacggt aaaaatgtag cgatgggttt cgcctttcgg cggcgctgcg
     1561 ccatcgtatc cggttttacc aaaatcggta cgcgtctgca aaacgccgtc tggcattgct
     1621 accagaccag agccaaaccc ttgcggtaat acgcgggtat cagcgggtaa attaacaact
     1681 acccagtgcc accagccgga gccggttggc gcatccgggt cgtagcaggt gacaacaaaa
     1741 cttttcgttc ccgcaggaac atcatcccac gccagatgcg gtgaaatatt atcgccatcg
     1801 taacccatgc cgttaaagac atgacgatgc ggcaacttat cgccatcgcg cagatcgtta
     1861 ctgatgagtt tcatgaaccc tcctttcttg tttgcagaaa gtgtagccag aaaccctcac
     1921 gctgacttcc cgttattggc aaaaaaatgt ttcatcctgt accgcgcggt taaccgctgc
     1981 ggtcagacgc tgcaactgtt gcgggagaat aatatagggc ggcatcaggt aaatcagttt
     2041 gccaaaaggc cggatccata ccccctgttc gacaaagaat ttttgcagcg ccgccatatt
     2101 caccggacga gtggtttcga ccacaccgat tgcccccagt acgcgcacat cggcaaccat
     2161 ttcggcatca cgggctggtg ccagttgctc gcgcagctgc acttcaatag ccgccacctg
     2221 ctgctgccat tcgccggatt cgataatcgc taggctggcg tttgctgccg cgcaggccag
     2281 cggattgccc ataaaagttg gcccatgcat aaagcagccg gcttcgccgt tactgatggt
     2341 ttctgcaacc tcgcgcgtgg taagtgtggc ggaaagggtc attgtgccgc cggttaaggc
     2401 tttaccgagg cacaaaatgt ccggcgcgat ttctgcatat tcacaggcaa acagtttgcc
     2461 ggtacgacca aatccggtgg cgatctcgtc ggcaatcagc aagatacctt cgcgatcgca
     2521 cattttgcgg attcgtttta accattccgg atggtacatg cgcatcccgc ctgcgccctg
     2581 gacaatcggc tcaatgatca ccgctgcgat ttcatgacga tgcgccgcca tcaggcgggc
     2641 aaaaccaacc atatcgcgtt catcccattc accatccatg cggctttgcg gggcgggagc
     2701 aaacaagttt tctggcagat agcctttcca caggctgtgc attgagttgt ccggatcgca
     2761 caccgacatc gcgccaaagg tatcgccatg ataaccattg cggaaggtca gaaaacgctg
     2821 gcgcgcttcg cctttggctt gccagtactg caacgccatt ttcatcgcca cttccaccgc
     2881 tacggaaccg gagtccgcga gaaaaacgca ctccagcggt tgcggcgtca ttgccaccag
     2941 tttgcggcat agctcgatgg cgggcgcatg ggtgataccg ccaaacatca catgcgacat
     3001 ggcatcaatt tgcgtcttca tcgccgcatt aagctgcggg tgattgtagc cgtggatcgc
     3061 cgcccaccag gacgacatac cgtcaaccag gcgtctgccg tcagacaaaa ttagctcgca
     3121 accttcggcg ctcgccaccg gataaaccgg cagaggggag gtcatggatg tgtatgggtg
     3181 ccagatatgg cgttggtcaa aggcaagatc gtccgttgtc ataatcgact tgtaaaccaa
     3241 attgaaaaga tttaggttta caagcctaca ccgaattaac aacaaaaaac acgttttgga
     3301 gaagccccat ggctcaccgc ccacgctgga cattgtcgca agtcacagaa ttatttgaaa
     3361 aaccgttgct ggatctgctg tttgaagcgc agcaggtgca tcgccagcat ttcgaacctc
     3421 gtcaggtgca ggtcagcacg ttgctgtcga ttaagaccgg agcttgtccg gaagattgca
     3481 aatactgccc gcaaagctcg cgctacaaaa ccgggctgga agccgagcgg ttgatggaag
     3541 ttgaacaggt gctggagtcg gcgcgcaaag cgaaagcggc aggatcgacg cgcttctgta
     3601 tgggcgcggc atggaagaat ccccacgaac gcgatatgcc gtacctggaa caaatggtgc
     3661 agggggtaaa agcgatgggg ctggaggcgt gtatgacgct gggcacgttg agtgaatctc
     3721 aggcgcagcg cctcgcgaac gccgggctgg attactacaa ccacaacctg gacacctcgc
     3781 cggagtttta cggcaatatc atcaccacac gcacttatca ggaacgcctc gatacgctgg
     3841 aaaaagtgcg cgatgccggg atcaaagtct gctcaggtgg cattgtgggc ttaggcgaaa
     3901 cggtaaaaga tcgcgccgga ttattgctgc aactggcaaa cctgccgacg ccgccggaaa
     3961 gcgtaccaat caacatgctg gtgaaggtga aaggcacgcc gcttgccgat aacgatgacg
     4021 ttgatgcctt tgattttatt cgcaccattg ccgtagcgcg gatcatgatg ccgacctctt
     4081 acgtgcgcct ttctgccgga cgcgagcaga tgaacgaaca gactcaggcg atgtgcttta
     4141 tggcaggcgc aaactcgatt ttctacggtt gcaaactgct gaccacgccg aatccggaag
     4201 aagataaaga cctgcaactg ttccgcaaac tggggctaaa tccgcagcaa actgccgtgc
     4261 tggcagggga taacgaacaa cagcaacgtc ttggacaggc gctgatgacc ccggacaccg
     4321 acgaatatta caacgcggca gcattatgag ctggcaggag aaaatcaacg cggcgctcga
     4381 tgcgcggcgt gctgccgatg ccctgcgtcg ccgttatccg gtggcgcaag gagccggacg
     4441 ctggctggtg gcggatgatc gccagtatct gaacttttcc agtaacgatt atttaggttt
     4501 aagccatcat ccgcaaatta tccgtgcctg gaagcagggg gcggagcaat ttggcatcgg
     4561 tagcggcggc tccggtcacg tcagcggtta tagcgtggtg catcaggcgc tggaagaaga
     4621 actggccgag tggcttggct attcgcgggc gctgctgttt atctctggtt ttgccgcgaa
     4681 tcaggcagtc attgccgcga tgatggcgaa agaggaccgt attgttgccg accggcttag
     4741 ccatgcctca ttgctggagg ctgccagttt aagcccgtcg cagcttcgcc gttttactca
     4801 taacgatgtc gctcatctgg cgcgactgct tgcttccccc tgtccggggc agcaactggt
     4861 ggtgacagaa ggcgtgttca gcatggacgg cgatagcgcg ccactggcgg aaatccagca
     4921 ggtaacgcaa cagcacaatg gctggttgat ggtcgatgat gcccacggca cgggcgttat
     4981 cggggagcag gggcgcggca gctgctggct gcaaaaggta aaaccagaat tgctggtagt
     5041 gacttttggc aaaggatttg gcgtcagtgg ggctgcggtg ctttgctcaa gtacggtggc
     5101 ggattatctg ctgcaattcg cccgccacct tatctacagc accagtatgc cgcccgctca
     5161 ggcgcaggca ttacgtgcgt cgctggcggt cattcgccgt gatgaggggg atgcacggcg
     5221 cgaaaaactg gtgtcactca ttgcgcgttt tcgtgccgga gtacaggatt tgccgtttac
     5281 gctggctgat tcatgcagcg ccatccagcc attgatcgtc ggtgataaca gccgtgcgtt
     5341 acaactggca gaaaaactgc gccagcaagg ctgctgggtc acggcgattc gcccgccaac
     5401 cgtacccgct ggtactgcgc gactgcgctt aacgctaacc gctgcgcatg aaatgcagga
     5461 tatcgaccgt ctgctggagg tgctgcatgg caacggttaa taaacaagcc attgcagcgg
     5521 catttggtcg ggcagccgca cactatgagc aacatgcaga tctacagcgc cagagtgctg
     5581 acgccttact ggcaatgctt ccacagcgta aatacaccca cgtactggac gcgggttgtg
     5641 gacctggctg gatgagccgc cactggcggg aacgtcacgc gcaggtgacg gcgttagatc
     5701 tctcgccgcc aatgcttgcg caagcccgcc ataaagatgc cgcagatcat tatctggcag
     5761 gagatatcga atccctgccg ttagcgactg cgacgttcga tcttgcatgg agcaatctcg
     5821 cggtgcagtg gtgcggtaat ttatccacgg cactccgcga gctgtatcgg gtggtgcgcc
     5881 ccggaggcgt ggtcgcgttt accacgctgg tgcagggatc gttacccgaa ctgcatcagg
     5941 cgcggcaggc ggtggacgag cgtccgcatg ctaatcgctt tttaccgcca gatgaaatcg
     6001 aagagtcgct gaacggcgtg cgttatcaac atcatattca gctcatcaca ctatggtttg
     6061 atgatgcgct cagtgccatg cgttcgctga aaggcatcgg tgctacgcat cttcatgaag
     6121 ggcgcgaccc gcgaatatta acgcgttcgc agttgcagcg attgcaactg gcctggccgc
     6181 aacaacaggg gcgatatcct ctgacgtatc atcttttttt gggagtgatt gctcgtgagt
     6241 aaacgttatt ttgtcaccgg aacggatacc gaagtgggga aaaccgtcgc cagttgtgca
     6301 cttttacaag ccgcgaaggc agtaggttat cggacggcag gttataaacc ggtcgcctct
     6361 ggcagcgaaa agaccccgga aggcttacgt aatagtgacg cgctggcgtt acagcacaac
     6421 agcagcctgc agctggatta cgcaacggta aatccttaca ccttcgcaga acccacctcg
     6481 ccgcacatca tcagcgccca agagggcagg ccgatagaat cattggtaat gagcgccgga
     6541 ttacgcgcgc ttgaacaaca ggctgactgg gtgttagtgg aaggtgctgg cggctggttt
     6601 acgccgcttt ctgacacttt cacttttgca gattgggtaa cacaggaaca actgccggtg
     6661 atactggtag ttggtgtgaa actcggctgt attaatcacg cgatgttgac tgcgcaggca
     6721 atacaacacg ccggactgac tctggcgggt tgggtggcga acgatgttac gcctccggga
     6781 aaacgtcacg ctgaatatat gaccacgctc acccgcatga ttcccgcgcc gctgctggga
     6841 gagatcccct ggcttgcaga aaatccagaa aatgcggcaa ccggaaagta cataaacctt
     6901 gccttgttgt agccattctg tatttggtta aattgcgagc gagatcgcgt cttcgattga
     6961 ctgcaattta accaattaaa ttctaaaata atcacgaaaa aaattttact tccgcctcat
     7021 gcggcgaatg tgggaattgc ccaggcggcg ggggataggg gctggagaca gttatccact
     7081 attcctgtgg ataaccatgt gtattagagt tagaaaacac gaggcaagcg agagaatacg
     7141 cggcttgcac gcgaattggc gttaaagacg gctcaaagaa atatctttta ttttttaact
     7201 ggttagataa atgcaatggc agtcactgaa caggcatctc ttgccataaa actgtcatca
     7261 ctcatcttga caaatgttaa aaaagccgtt gctttgggga taacccggta aggctggagt
     7321 tttatctcgc cacagagtaa attttgctca tgattgacag cggagtttac gctgtatcag
     7381 aaatattatg gtgatgaact gtttttttat ccagtataat ttgttggcat aattaagtac
     7441 gacgagtaaa attacatacc tgcccgccca actccttcag gtagcgactc atgagtaaac
     7501 cgttcaaact gaattccgct tttaaacctt ctggcgatca gccagaggcg attcgacgtc
     7561 tcgaagaggg gctggaagat ggcctagcgc accagacgtt acttggcgtg actggctccg
     7621 ggaaaacctt caccattgcc aatgtcattg ctgaccttca gcgcccaacc atggtacttg
     7681 cgcccaacaa aacgctggcg gcccagctgt atggcgaaat gaaagagttc ttcccggaaa
     7741 acgcggtgga gtatttcgtc tcctactacg actactatca gccggaagcc tatgtaccga
     7801 gttccgacac cttcattgag aaagatgcct cggttaacga acatattgag cagatgcgtt
     7861 tgtccgccac caaagcgatg ctggagcggc gtgatgtgat tgtagtggcg tctgtttccg
     7921 cgatttatgg tctgggcgat cctgatttat atctcaagat gatgctccat ctcacggtcg
     7981 gtatgattat cgatcagcgc gcgattctgc gccgactggc ggagctgcaa tacgctcgta
     8041 atgatcaagc attccagcgt ggtactttcc gcgttcgtgg cgaggtgata gatatcttcc
     8101 cggcagaatc ggatgacatt gcacttcgcg tggaactgtt tgacgaggaa gtggaacgat
     8161 tgtcgttatt tgacccgctg accgggcaga ttgtttccac tattccacgt tttaccatct
     8221 acccgaaaac gcactacgtc acaccgcgcg agcgcatcgt acaggcgatg gaggagatca
     8281 aagaagaact ggccgccaga cgcaaagtgc tgttggaaaa caacaaactg ctggaagagc
     8341 agcggctgac ccagcgtacc cagtttgatc tggagatgat ggacgagctg ggctactgtt
     8401 cggggattga aaactactcg cgcttcctct ccggtcgtgg accgggtgag ccaccgccga
     8461 cgctgtttga ttacctgcct gccgatgggc tgctggtcgt cgatgaatct cacgtcacca
     8521 ttccacaaat tggcggcatg tatcgcggtg accgggcgcg taaagagaca ctggtggagt
     8581 acggcttccg cctgccatca gcgctggata accgtccgct taagtttgaa gagttcgaag
     8641 cattagcgcc gcaaaccatc tatgtttcgg cgacgccggg taattacgag ctggaaaaat
     8701 ccggcggcga tgtggtggat caggtggtgc gtccaaccgg attgcttgac ccgattatcg
     8761 aagtgcggcc ggtggcgaca caggttgatg atcttctttc ggagattcgt cagcgagcgg
     8821 caattaacga acgcgtgctg gtcaccacac tgaccaagcg aatggcggaa gatcttgccg
     8881 aatatctcga agaacacggt gagcgcgtgc gttatcttca ctcagatatc gacaccgtcg
     8941 agcgtatgga gattatccgc gacttgcgtc tgggtgagtt cgacgtattg gtagggatca
     9001 acttactgcg tgaaggtctg gatatgccgg aagtgtcgct ggtggcgatc ctcgacgctg
     9061 acaaagaagg cttcctgcgt tccgaacgtt cgttgatcca gaccattggt cgtgcggcac
     9121 gtaacgttaa cggtaaagcg attctctacg gcgataggag cactccatca atggcgaaag
     9181 cgattggcga aaccgaacgt cgccgtgaga aacagcagaa gtacaacgag gaacacggca
     9241 ttacgccgca aggcttgaac aagaaagtgg tcgatatcct ggcgctgggg cagaacattg
     9301 ccaaaaccaa agcgaagggc agaggaaaat cgcgcccgat tgttgagccg gataatgtgc
     9361 cgatggatat gttgccgaaa gcgttgcagc agaaaatcca tgaactggaa gggctgatga
     9421 tgcaacacgc gcagaatctg gagttcgaag aagcggcaca aattcgtgac cagttgcatc
     9481 agttgcgtga gttgtttatt gccgcatcgt aacaggatag cgaagaagac tgatgacaaa
     9541 cggaaaacag tctgatgcgc tacgcttatc aggcctacat tttctccgca atatattgaa
     9601 tttgcgcggt ttgtaggccg gataaggcgt tcacgccgca tccggcataa acaacgcgca
     9661 ctttgtcttc aataggaagc cggaattttc cttcggattt ccgttaacct aaagcctgta
     9721 acgccttttc cagcgcgtta tgtaacaact ggcggtcatg acgatacgga atatcgctgg
     9781 cctccagtac ctcctggatc acaatccgct ctttcaccgc cgagacatcc acttttggcc
     9841 cgacgatgac cgcatcaatg acctttttac caacatactg ctccataatt gccagcttgc
     9901 tttccagctt caaattagcc gcaggtaaac tcaactcacg ccccagattg ccgatataaa
     9961 ccatcggcgc tggcgtgcgg cgtaatgcct gggcgatttc cttcagcagc agaattggca
    10021 tcaggctggt ataaaaactg ccaggcccaa taatgatgag atccgcttca ttgatagcgt
    10081 gaaccgcctc acgcgttgcg ggtacattag gcgttaacaa taactcttga atcggcgtag
    10141 ttaactggtc gatattgacc tcgccgtaaa cttcatgccc ctgatcgtca atcgccatca
    10201 gatcaacagg atgctctgac attggaatca aatgcgtatc cactttcagc agattacgaa
    10261 ttaaattgat ggcttccaga ggccgcacgc taaggtgatc cagcgccttt aacatcaagt
    10321 ttccgagatt atgaccggaa agttcgccat tgccgccaaa acggtattca aacatcgcgg
    10381 aggcgacgtt cggttccgtt atcagctggt tgaggcagtt gcgcatatcg ccccaagcaa
    10441 tgccgccttc tgaacggcga atacgccccg tcgagccacc attatccgtg gtggtgacga
    10501 tacccgttaa acgagaaccc aaagacgaaa gtgatgagag aacgcgtccc agtccatgcc
    10561 ctccgccgag agcaacgaca cgatcaagat cagccagcgt acgattgcgc atatagtttc
    10621 ctggagtcag attatccgcg ctacagtagc gcaaattgcg gggaaacagc aattaacctg
    10681 ccaatattga ggatacaaac tattttcttc tatctctaaa ggacgatgca cgctatgcct
    10741 ccctgatgat gtatatcaaa gtaaaaccgc cattttccct tattctgtag cgaaatagca
    10801 cgatcatgac gctatataca tgattacata gcgaaagtgt ggatggtaaa aatctcattt
    10861 acacgctagt atcggcttaa ccactaaaca ctctagcctc tgcacctggg tcaactgata
    10921 cggtgctttg gccgtgacaa tgctcgtaaa gattgccacc agggcgaagg aagaaatgac
    10981 ttcgcctccc gtatctggaa aggtgtacat ggcttcacaa ctgactgatg catttgcgcg
    11041 taagttttac tacttgcgcc tgtcgattac cgatgtgtgt aactttcgtt gcacctactg
    11101 cctgccggat ggctacaaac cgagcggcgt caccaataaa ggctttctta ccgtcgatga
    11161 aattcgccgg gttacgcgcg ccttcgccag tctgggcacc gaaaaagtgc gcctgacagg
    11221 aggagagccg tctttacgcc gcgactttac cgatatcatc gccgctgtgc gggaaaacga
    11281 cgctatccgc cagattgcgg tcaccaccaa tggttaccgt ctggaacgcg atgtggcgaa
    11341 ctggcgcgat gcgggactta ctggcattaa cgtcagtgtc gacagtttgg acgcccgcca
    11401 gtttcacgct attaccgggc aggataaatt caaccaggtc atggcaggga ttgatgctgc
    11461 atttgaggcc ggttttgaga aggtcaaagt caataccgtg ctgatgcgtg atgttaatca
    11521 tcaccagctc gacacctttc tgaactggat ccagcatcgc cctatccagc tgcgtttcat
    11581 cgaactgatg gaaacgggcg agggcagcga gctcttccgt aagcatcaca tctctggtca
    11641 ggttctgcgt gacgagctac tgcgtcgcgg ctggatccac caattacgtc aacgcagcga
    11701 cggtcccgcg caagtctttt gccatccaga ttacgccgga gagattggcc ttatcatgcc
    11761 gtatgaaaaa gacttctgcg ccacttgcaa ccgcctgcgc gtttcctcca ttggtaaact
    11821 ccatctctgc ctgtttggtg aaggcggcgt taacctgcgc gatctgctgg aaggcgatac
    11881 ccagcaacag gcgctggaag cgcgtatttc agcggcgctg cgggagaaga aacagaccca
    11941 tttcctgcat caaaacaaca ccggtattac gcaaaactta tcgtacattg gcggctaaaa
    12001 cgtcaaaagg agagatcaga tgagtcaggt aagcactgaa tttatcccga cccgtattgc
    12061 tattcttacg gtttctaatc gtcgcggtga agaagacgat acctccggtc actatctgcg
    12121 cgattcggcg caagaagcgg gccatcacgt tgtcgatgaa gccattgtga aagaaaaccg
    12181 ctacgctatt cgcgctcagg tatctgcgtg gatcgccagc gacgatgtac aagtggtatt
    12241 gattacgggt ggtactggcc tgacggaagg tgatcaggct cccgaagcgc tgctgccgtt
    12301 gttcgaccgt gaggttgaag gttttggtga agtgttccgt atgttgtcgt ttgaagagat
    12361 tggcacttcc acgttgcaat ctcgtgcggt agcgggcgtc gctaacaaaa cgctgatttt
    12421 cgccatgccg ggttcgacca aagcgtgccg taccgcatgg gaaaatatca tcgcgccgca
    12481 gctggatgcc cgtacgcgtc cgtgtaattt ccatccacat ttgaagaaat aagtatgtcg
    12541 caactgaccc atatcaacgc cgctggcgaa gcgcacatgg tggatgtctc cgccaaagcg
    12601 gaaaccgtgc gtgaagcgcg ggcggaagcc tttgtcacca tgcgcagcga gacgctggcg
    12661 atgattattg atggtcgcca ccacaaaggc gacgtatttg ccactgcgcg tattgccggt
    12721 attcaggcgg caaaacgcac ttgggatctg atcccgctgt gtcacccgct gatgctcagc
    12781 aaagttgaag tcaatttaca ggccgagccg gagcacaatc gggtgcgtat agaaacctta
    12841 tgccgcctga ccgggaaaac cggtgtcgaa atggaagcgt taaccgcggc ctccgtggcg
    12901 gcgctgacca tttatgacat gtgcaaagcg gtgcaaaaag atatggtgat tggtccggta
    12961 cgtttgctgg cgaagagcgg cggcaagtcg ggtgacttta aggtggaagc ggatgattaa
    13021 agttcttttt ttcgcccagg tgcgcgagtt ggtgggaaca gatgcaaccg aagtggctgc
    13081 ggattaccca accgttgaag cgttacgcca gcacctggct gcgcagagcg atcgctgggc
    13141 gctggcgctg gaagatggca aattgctggc tgccgtcaac cagacgctgg tgagttttga
    13201 ccatccgctg actgacggcg acgaagtagc tttcttcccg ccggtaaccg gaggttaaga
    13261 tggcagaaac caaaattgtt gtcggcccgc agccgttcag cgtaggagaa gagtacccgt
    13321 ggctggcgga gcgtgacgaa gacggtgcgg tagtcacctt caccggaaag gtgcgcaacc
    13381 ataacctggg cgacagcgtc aaagcattaa ccctcgaaca ctatccgggg atgactgaaa
    13441 aagcactggc agaaattgtt gatgaagcgc gtaaccgctg gccgctgggg cgcgtcacgg
    13501 tgattcaccg catcggggaa ttatggccgg gcgatgaaat cgtttttgtc ggtgtcacca
    13561 gtgcgcatcg cagcagtgcg tttgaagccg ggcagtttat tatggattat ctcaaaaccc
    13621 gcgcaccgtt ctggaagcgc gaagccacgc cggaaggcga ccgctgggtt gaagcccggg
    13681 agagcgatct gcaggcggca aaacgctggt agttttttgt tagccggata aggcaccgct
    13741 gcgtccggca atttcaccgc tcagtacatg cgtttacgct gcgtcgatgc acagcctcat
    13801 cattttgcag tatccttaag atattcctta tatcttcagg agatcgtcat ggacagattc
    13861 ccacgttctg attcaatcgt acaaccccgg gctggcttgc aaacttatat ggcgcaagtc
    13921 tatggctgga tgaccgttgg cttgttgctg accgcatttg ttgcctggta tgcggctaat
    13981 tccgcggccg tgatggagct gttgttcact aaccgtgtct ttttaatcgg tctgatcatc
    14041 gcgcaattag cattggttat tgtgttatca gcgatgattc aaaagctgag cgcaggtgta
    14101 acgacgatgc tctttatgct ttattcggcg ctgacgggtc ttacgctttc cagtatattc
    14161 attgtctata ccgctgcttc tatcgccagt actttcgtcg ttactgccgg gatgttcggc
    14221 gcaatgagcc tgtacggtta caccacgaag cgcgatttaa gtggctttgg caatatgctg
    14281 tttatggcgt taatcggcat tgtgctggca tcgctggtca acttctggct gaaaagcgaa
    14341 gcattgatgt gggcagttac ctacatcggc gtgattgtct ttgtcggatt gacggcgtat
    14401 gacactcaga aattgaaaaa tatgggtgag cagattgata cccgcgacac gtcgaacctg
    14461 cgcaaatatt ccattcttgg tgcgttaacc ttgtatctgg acttcatcaa cctgttcctg
    14521 atgttgttac ggatcttcgg caaccgccgt taattctttt tcaccatcac tatcccggcc
    14581 atgtgtcggg atttttttct tactaaaaac gacacttaac aagtaccagg tataaaatat
    14641 gccttatgag cgagcggaat cgttttccgt atccataaat acaaaagtat tttgtatgcg
    14701 tggttttaaa tttattgatg gtgaatatta atgttttttc taaggacaac taaaagaatg
    14761 gaatcatact cgcaaaacag caataaatta gattttcagc acgaggccag gatattaaac
    14821 ggtatatggc tcattaccgc tttaggtttg gtggcaaccg caggactagc ctggggagct
    14881 aagtatctcg aaattacggc aaccaaatat gattcaccac caatgtatgt cgccataggg
    14941 ttattattac tttgtatgta tggcttaagt aaggatatca acaagataaa tgccgccatc
    15001 gcgggcgtaa tatatctgtt tttactctct ttggtggcga ttgtcgttgc aagtttagtt
    15061 cctgtatatg ccattatcat cgtgttcagc actgcgggcg cgatgttttt aatcagtatg
    15121 ctggccggtt tattatttaa tgttgatcct ggttctcacc gttttatcat tatgatgacg
    15181 ttgacagggt tggccctggt aatcatcgtg aatgcggcat taatgagtga acggcccatt
    15241 tgggtaataa gttgcttaat gattgtgtta tggccaggca ttatctcgca tggacgaaat
    15301 aagctccttg aacaggcagg aaaatgccat agtgaagagt tgtggagtcc ggttcgttgc
    15361 gcttttacag gtgcattaac actctattac tattttatcg gcttctttgg gatacttgcc
    15421 gcgatagcta taacgcttgt ctggcaaagg catacgcgtt tttttcatta gtcgtaaatg
    15481 cagatactaa agaacttaac taaacttcac atcgccgctt catttttcgc ccgtagcttc
    15541 ttcgcttggc tttccagtag caggtaacag atcagcgcca gcagcagcgg gataaagtaa
    15601 tacaggacgc gataagcgag cagggcggcg ataatgctcc ctttcgaggt atgctcccca
    15661 gccagtagtg cgataaacac cgcttccagc acaccgatcc ccgccggaat atgcacgatg
    15721 acgccagcaa tactactaac cagtaacacg cccagtacaa agaaatagtt cacgctttga
    15781 ccaagtaaca gccagataat cgcccccatt accatccagt taacgctgga aatcagcatc
    15841 tgagcgaggg cgaatttcca tgaaggcagc accagctttt gccctttgat ggtcatatgg
    15901 cggtgcttcg cgaaagcgca aaaccacaaa taaaccgcga taatcatcag taagccaatg
    15961 ccgagaatac gcagcgtagt ttgatcgaca taccagtaat ccggcaactc caccacgcct
    16021 gcggtaaaga taatccctgc cagtaaaata tagcccagcc agttggtggt aatactgagc
    16081 gaggaaatcc gcgtaatagt gctgcccggt aaccctagtc gggagtacaa acgatagcgc
    16141 ataccaatgc cgccgaccca ggtactgagc gtcaggttga aggcgtagca gataaacgac
    16201 accagcatca cctggcgctt cgccagtttg tgaccgcagt aaaagcgggc gagcaggtca
    16261 tagcagccgt aaatcagata gctgacgacc accagcccga ccgcactaag cagcgcaacg
    16321 cgattgtagt cgcggatgac cttccagacc tcttcccagt ccactttttt ggcgtagacc
    16381 accagcaaca caatcaccgc gataaaaaac agccaggtga ggatcttctt tgctaagcgc
    16441 cagcgcgggt gtgatttact catcaggggt ttacccccgt gttttcagtt tctacccgat
    16501 cctgcgtttc cattgtcggt tgtgcggggg gatcaacctg cgtcagacgt ggtgtgtgtg
    16561 ccggaagcca gccaaccagc gccgggaagt ggcgtaaaaa gtggaacgcc agcacgcttt
    16621 tggtcaggtt ccaccaggtg cgtttgggca gcatggtttc atccacctgc tgacaatctg
    16681 cggcaataat gccgttcaga ttatcgcgca gcgtctggtt aaaatgacga tcgtggatga
    16741 tgacatttgc ttcgagattc agtgacaaac tgagcggatc gagattactg gaccctactg
    16801 tcgcccagtg atcgaccatc aatgccactt tgccgtggag cgggcggcgg cggtactcaa
    16861 aaacctgaat gccgccttta accagatagt tatacagcaa gcgcgcaccg actctgacaa
    16921 tcggcatatc cggttcgccc tgaatgatca gtttgatccg caccccgcgc cgtgccgctt
    16981 tacgcaaggc gtgtaaaaat cgatagccgg ggaagaagta ggcgttggcg ataatcactt
    17041 cccgctgcgc ctgagtgagc attttcagat aatggcgttc aatatcatcg cgatgttctt
    17101 cgttatcgcg ccagaccagc aatacctgcg cttctcccgg ctggcggttc tcttccgctt
    17161 tgtgatgacg tcgccaccag cgtcgtgccg cgctctgtcc aggcaggttt tccagctcaa
    17221 actggagaat atcttcgaca atcggccctt caaggcgtac cgcgtaatcc tgtttagcct
    17281 ctggaccgta gctggacata tgctcggcgg agtaattcag cccgccaata aaggcgatac
    17341 gcgcgtcgat caccacaatt ttgcgatgca tccggcgaaa cacattggtg cgcataccaa
    17401 aaaggcgagg gcggggatcg tagtagcgga acactacgcc agctgccgtc agttcattga
    17461 caaactcatc gctgagatcc ggcgaaccgt agccatccag caagacttcc gctttaaccc
    17521 cgcgttgcgc tgctgccagt agtgccgcat gcagctgttt gccgacgtta tcctcaaacc
    17581 agataaacgt ttcaagaatg atgcgttctt gtgcctcgcc aatcgcctta aacaccgcgg
    17641 gataatattg ctcgccgttt tccagcaact ggatcttatt gccttcgcgc cagctacatt
    17701 tcataaatga atctccgcac ttaaaggggc atgatcagaa aggtgtcgcc atgtccgcag
    17761 cggcaacgcg gttggcgcgc tggcgctggc atttttgacg tagatcctgt ccagtcgtag
    17821 tagaggaaat tgcaccggaa acgtgcgcgc cgggcgtccg tgggcgcggg taaaaatctc
    17881 atccagtccg gcctgcactt ttaacggatg attagctttt tgccgccagt cattgaaatc
    17941 acccgccacc aataccggtt cgccgtccgg tagctcattc acccattcgg cgagcatcgc
    18001 aagctgcgcc tgacggtgcg cctcacgcag gcccagatgt acgcacatca catgaatcgc
    18061 ttttccggtc atcggcggca caatgcggca gtagagcacg ccgcgctttt ccgcaccatc
    18121 gaccgaaaca tcgcgattct cataatgttc aatgggataa cgcgacagta cggcgttgcc
    18181 gtgatgccct tccgggtata cggcattgcg accgtaggca aaatcgctcc acatagtgtc
    18241 ggcgagaaac tcgtagtgcg aggtatcggg ccagttttcc acatgcagcg gatgaacttc
    18301 gtgcgcgccc atcacttcct gcaggcaaac aatatcggcg ctgacggtac gcacggcatc
    18361 gcgaagttcc ggcaaaatga agcgtcggtt aaacgcggta aagcctttgt gaatgttgat
    18421 ggtgagcacc ttgaacgaaa attgttgtgt ttgatcgggc ataattttcc tgtctttgcc
    18481 tcttatctca ttgaaatagt gtagtcggcg tcacaaaaag gtgcggtctt acggaatttt
    18541 ccgtaaagtt cggtactctg agtaagtaga gataaattct tcaggagaga agccatgaag
    18601 tggcaacaac gtgttcgtgt cgcaacgggt ctaagttgct ggcagattat gttgcattta
    18661 ctggtagtgg cgctgctggt ggtgggctgg atgagtaaga ctctggttca cgtcggcgtg
    18721 ggattatgcg cactgtattg tgtcacggta gtgatgatgc tggtgtttca gcgccacccc
    18781 gagcaacgct ggcgtgaggt ggcagacgtg ctggaagagc tgaccacgac ctggtatttt
    18841 ggcgcagcgc tgattgtgct gtggctgttg tcccgcgtac tggaaaacaa ctttttgctg
    18901 gcaattgcag ggctggcgat ccttgctggc ccggcggtag tgtctttgct ggcgaaagat
    18961 aagaagttac atcaccttac gtctaaacat cgcgtacgcc gctgaccctg tcgtggccgt
    19021 tatcaccagt agcggccaca aactattcca cacaatatcc agactcgcat ccttcaaata
    19081 aatctgcttg gtaatgtccg taaagtggcg aatagggtta atccacgtca gattttgcag
    19141 ccacactggc atgttttcca ccggcgaaac gtaaccggaa aggagaatgg cgggcatcat
    19201 aaaaacaaac acgccgataa acgcctgctg ttgtgtcgag cagagtgatg aaatcaacag
    19261 accgaatccc accagcgata aaccgtaaat caccatcgta aagtagaaca gcgctagcga
    19321 tccggcgaag gggatttgat acgcccagat accaatcgcc agcacaatgg tggcctggaa
    19381 ggtggcgaca attaacgccg gtacggcttt gccgatgaag atctgccagg tggtgagcgg
    19441 cgaaaccagt agctgatcga gcgtaccttg ttcacgttcg cgggcgacgg aaagtgaagt
    19501 gacgatcatt acgccgatag tggtgatcat ggcgatcagc gacggcacca caaaccattt
    19561 gtagtcgaga ttcgggttat accagttgcg taccaccagc tcgctgttgt tgggtttcgg
    19621 ttttccttcc agcagctcct gctgataatt tttgacgatc tgttgcaggt agttggcggc
    19681 aatttgcgca ctgttggagt tacgcccgtc gaggatcaac tgcaacggcg cggtctggaa
    19741 ggtatccagt ttgcgcgaga agtcagccgg gaaacgcacc agtagtaacg ccttttgtgt
    19801 gtcgatggtc gggcggatct cctgtgggct tttcagcagc agcacatgtg taaaggcgct
    19861 ggcgcgggca aaacgttgag taagttccac cgaatgctcg ccgttatctt catcgtagat
    19921 ggcgatggtg gcgttagtca cttccagcgt ggcggcgaac gggaacagga tcacctgaat
    19981 tagcacgggt aaaatcagaa tcgcgcgggt ttgtggttcg cgcagcaacg actgcaactc
    20041 tttgcggatt aacgtccata agcgatgaaa catgctcttc tccctaatcc agccgacgtt
    20101 tggttttcag ccacgtcagg ccgataaaca tcaccgccga agcgatcaaa aacagcacgt
    20161 ttaccaccag caccactgga atattcccgg cgaggaacag gctttgcagg gtgctgacga
    20221 aataacgagc gggaataatg tacgtcaccg cgcggatcac cgcgggcata ctgtcgatct
    20281 gaaaaataaa gccggaaagc ataatcgacg gcagaaaagc ggcgttcagg gcgacctgag
    20341 cggcattgaa ctggttgcgg gtaatcgtgg aaatcagcag ccccattccc agggtactga
    20401 gtaaaaacag gctggagata aaaaacagaa tcagcagcga accgcgatac ggcacgccga
    20461 gaataaacac tgacaccagc atacacagca acatcgccag catcccgaga aagtaataag
    20521 ggatcagctt acacagcagc agttccgtgc gggtaatctc cgtagagagc agagcctcca
    20581 tggtgccgcg ttcccattct cgcgccacca ccagcgaggt gagaatcgcg ccgatgaccg
    20641 tcatgataat ggtcaccgca cccgggataa taaagtgctg gcttatcgcc gccgggttaa
    20701 accagtagcg ggtttgtaca tcaataagcg gttcaaaagt ctgcccgttg tcctccgctc
    20761 gctgcatttg ccagatctgc cagatccctt cgacataccc ctgcacaaag ttagcggtat
    20821 tcggctcact gccgtcggtg atcacctgaa tcggtgcggt ggcgttggcg cgctccatct
    20881 gttcggcgaa atccacggga ataaccacca gaccgcgaat tttccccgac tgcattttgg
    20941 cgatcagttc ctgacggtta tcgctgatgg tggcgtcgat gtagggcgaa ccggtcatgg
    21001 tgtgggtgaa atccagcgcc gcttcgctac gctgttccag caaaatcccg acccgcagct
    21061 tgctggagtc gaggttaatg ccgtaaccaa aaataaacag cagtagcagt gggatcacta
    21121 ccgcaatcag ccagctactc ggatcgcgaa cgatctgccg cgtctctttg acgcacagcg
    21181 cccgcacgcg atgccaggaa aggatcgggt tactcattgc tatgctcctt atcccagtcg
    21241 tggatcaact gaataaaggc ttgctccatg gtgggatcgg gttgctcatc gttagctgac
    21301 tgtgctttca aatcgtccgg cgtaccgctg gcgattaatt tcccatggta caccaggccg
    21361 atgcggtcgc aatattccgc ttcatccata aagtgggtgg tgaccatcac cgtgacgcct
    21421 ttctctacca tgctgttgat gtgcagccaa aattcacggc gggtgagggg gtcaacgccg
    21481 gaagtcggtt cgtcgagaaa gagaatatcc ggttcatgca tcagcgaaca ggccagcgcc
    21541 agccgctgtt taaaacctaa tggcagttca tcggtggcgt gtgaggcaat acttttcagg
    21601 ccaaatgcct cgctcatacg ggagattttt tcgttctgcg cccgaccgcg taagccatac
    21661 acaccagaga aaaagcgtaa attctgttcg accgtcaggt tgccgtagag cgaaaatttt
    21721 tgcgccatat agccgagatg ctggcgcgct ttaccggaac tctctttcag atccatcccc
    21781 agcaccagcg cctggccgga agtcggcacc agcaaaccgc acatcatctt aaaggtggtc
    21841 gatttacccg cgccgtttgg ccccagcaaa ccaaaaatct ccccacgttt aacggcaaag
    21901 ttgacgtgat cggtggcggc aaaatcacca aatttcttgg tcagttcttt cgcttcgatc
    21961 accgtctcgc cgggtgtgcc ttctaccgta tgcaatattg cacccagcgg cgattctgag
    22021 gttccggcac cgcccagcaa atcaataaac gcatcttcaa aacgcggcgt agtttcgttg
    22081 atgttgattt ccggcatccc gtcggcatgg cgaatatcgt ctggtgtggc ctcttttttg
    22141 aggatcagac gtaccgattt cccctgaatc atgccatcgc tgacctgcgg cagtttcaag
    22201 gcgcgttgca acagtttgcg gttgccctcg tgtggactgg tcatcagaaa gctgcgtccg
    22261 gccatggttt gtgtcagggc ttttggttct ccctgataca gcaactcgcc ttcgttcatc
    22321 agtaacacgt cacggcactg ctcggcttcg tcgagatacg aggtactcca gaggattaac
    22381 atcccttcgc ccgccagctc atgcaccatc tgccacagtt cgcgccgtga gatagggtca
    22441 acgccgacgc cgggttcatc gagcagcaac actttcggtt cgcccaccag ggtacaggcc
    22501 aggccgagtt tttgtttcat cccaccggag agcttgcccg ccaggcgtcc ggtaaacggc
    22561 ccaagagacg taaactccag caggcgagca aaagtttgct tacgtgcctc gccggtgacg
    22621 ctgcgcaaat ccgcgtacag attgaggttc tccatcaccg tgagatcttc atacagacca
    22681 aatttctgcg gcatatagcc gagcacggcg tgcagcgcgc cgtcgttttt gatcggatca
    22741 aagccaatca ccgtggcact gccgctgtcg ggtttcagta atcccgccaa catccgcatc
    22801 agtgtggttt tacctgcacc gtccggcccc accaaccccg tcacataacc ggcgtgaatg
    22861 gtacaatcga gcggcgcgac ggcgggcttg tccatgcccg gaaagcgttt ttccaggccg
    22921 ttcagcgtga taacggcatc attcatgtcc tgcctcgtca ccgaattgta ccgtcactgg
    22981 cattccctgg cgtaacgcat catcggcgtc ggtcaccaca atacgcaggc gatagacgag
    23041 gtcggtacgc agatccggtg tttcgacggt tttcggggta aattcagcag tcggcgaaac
    23101 gaaaccaatc tgcccgtgat acggcttgtc cgggcgacca tcggtataaa gcagcacttt
    23161 gcgccccggc tgggcctggt caagattccg ttcatcaaca taagcgcgca cccacaccgg
    23221 acgcgttagt gaaacggtaa acaccgtgcc accttcattg agaaccgtgc ctggctctac
    23281 cgcgcgcgtt aacagcgtgc catcagacgg tgctatcaac gttgagtcct gtaaattcag
    23341 ctccgcctgc gccagttgcg cctgcgcctg ttcgaggctg gctttcgcct gagcgatgtc
    23401 ctgttcacgg ttaccggaac ggtactgacg caatttatcc tgtgctgatt tcagcgttgc
    23461 ctgtgcctgg tcgcgcgagg agcgggcatt ttccaggtca tttgccgaaa tggtgcggct
    23521 tttccacagt ccttgctggc ggttatagaa gttctgcgca tagtcatagg cggcttgcgc
    23581 ctgtttcacc gctgcggcgg cctgagcgat ttcttcatcg cgatacccgg caagcatcag
    23641 attatactgc gcctgtgcca ccgagacgcc cgctttcgcc tgcatcaggg caatctcata
    23701 cggcttgtga tccagttcgc ccagcacctg gcccgctttg atagcatcac cttcgtccac
    23761 cgccagcgat tcaacgcgcc ccccaacacg gaaactaaga ttcaccgtac gaatatccac
    23821 gttgccatac agcgtcaggc cgttatcctg gcggctttga taccaccagt agcctccggc
    23881 aaccacggcg gcaagtacca ctaccgccag tccgatcacg acaggttttt tcatcactac
    23941 aaactccttt gcgataatcc ttgcaaaatc aggtcgatat gacaggtcac cgtctggttg
    24001 atcagttcgg tcttttcttc atcgaacgcg gtccagccgg tacgtaacag aattgtttct
    24061 ttgccaagac ggaacgccag aatctcacca atcaacgcat gggtatgaag gatcatgcgg
    24121 gtgtcattag cgtcgcagcc ggtccaggcg gcaatcaggc gtgtcaggtg gctgtgtagc
    24181 ggactaatca cctgttcgtg caccaggtgg taggctgccg tgggagagag ctgctcacgg
    24241 gagataaact tgctgaggtt gacggtgtca tcctgggtga gcagcttaat catgttcctg
    24301 caggcgcgaa ggatcagttc acggatggca gcccgatcag gctgtggttg ggcgaacaag
    24361 cgttcggctt cctcggcatg cggacggaac tgctcgccaa taaaatcggc aatccactgg
    24421 gcgcaggcga ggtacaaatc ttctttcgaa ccgaagtagt aggtgatggc agcgatattc
    24481 tgcccggcct gggcggctat ctcgcgggtg gtggcgttca ttccatattc accaaactgc
    24541 gccagtgcgg cagcaatcag ctgttttttc gcctgttcac ccttgattgt catggcagga
    24601 ttattcatcg cacagtccat tcttaatcaa atgattgatt aagattatga ctccatatgg
    24661 gagttgtcca gtatggctaa gaattttagc tacgccagtc acagggataa tttatgcgct
    24721 gcgtcacaaa aactgctaca ctccgctccc tcatgacatt gtggtttttg tcattttcct
    24781 tttcagtatc tccctgaaaa ctacaccggt aacggtcggg gcggttcgga gtagttatgt
    24841 ctttcgattc tttgggttta agccctgata tcctgcgcgc cgttgccgag cagggttacc
    24901 gtgaacccac ccctattcag cagcaggcga tccctgcggt gctggaaggc cgcgacctga
    24961 tggctagcgc ccagaccggc accggcaaaa cagcgggctt tacgctgccg ctgttgcaac
    25021 acctgatcac tcgccagccg cacgccaaag ggcgtcgtcc ggtacgtgcg ctcattctta
    25081 ccccgacccg tgaactggcg gcacagattg gcgaaaacgt ccgtgattac agcaaatacc
    25141 tgaacattcg ttcgctggtg gtgtttggcg gtgtcagcat taacccacaa atgatgaagt
    25201 tgcgcggcgg cgttgatgtg ctggtggcaa cgccgggacg tttgctggac ctggaacatc
    25261 aaaatgcagt gaagctggat caggttgaaa tcctcgtcct cgatgaagct gaccgcatgc
    25321 tcgacatggg ctttatccac gatatccgtc gcgtgttaac aaaactgcca gcgaaacgcc
    25381 agaacctgct gttctctgcg accttctctg acgatattaa agccctggcg gaaaaactgt
    25441 tgcacaaccc gctggaaatc gaagtggcac gccgcaatac cgcgtctgat caggtgaccc
    25501 agcacgttca ctttgtcgat aagaaacgca aacgcgaatt gctgtcgcac atgattggta
    25561 aagggaactg gcagcaggtg ctggtgttta cccgtaccaa acacggcgct aaccatctgg
    25621 ctgagcagct caataaagat ggcatccgta gtgtggcgat ccacgggaat aaatcgcaag
    25681 gtgcgcgtac ccgtgcgctg gctgatttta aatcgggtga cattcgcgta ctggtggcga
    25741 ccgatatcgc tgcgcgcggt ctggatatcg aagagttgcc gcacgtcgtc aaccatgaac
    25801 tgccaaacgt accggaagat tatgtccacc gtatcgggcg taccggtcgt gcggctgcta
    25861 ccggtgaagc gttgtcgctg gtgtgtgttg atgaacacaa actgctgcgt gatatcgaaa
    25921 aactgctgaa aaaagagatc ccgcgcattg cgattccggg ctatgagccg gacccgtcaa
    25981 tcaaagccga accgatccag aacggtcgcc agcaacgtgg cggcatcagg ctgatgaaca
    26041 aacgcaaaac tgcctgatgc gctacgctta tcaggcctac gtgaactctg caatatattg
    26101 aatttgcatg cttttgtagg ccggataagg cgtttacgcc gcatccggca tttcacaaca
    26161 agcacttgtc agcaatttga gaacacggga aaaaatttat ctgttttacc gcccataagc
    26221 caccgagacg ttacttctct atagccaatt gttcgcgcaa ctccattaaa aggtagccca
    26281 gtcgattctt gcccttacca ttaccaccgt ctccccagta agcatcgttt tccgtatgct
    26341 caaccagttt tgcgggcgcg gttgccagca agagcacacg cagttctgca tgctgttcga
    26401 atttagcgcg aagtgcttta cgcatcacct gttctttaac cgactcccag tttttacgaa
    26461 gtggcttgga acgattacgc cccatgcgag cggcgaccat gggggaagaa acccgacgga
    26521 tctcttcgcg gtatttttca tcaaggaatt tttgtgcctg aaaatagtgt tcggaggtgg
    26581 gccaggtatt cccgtcaacc ttgatgggcc tggcggcaaa gttagaaaaa tcaccgtagt
    26641 catcgctggt gctgtagaaa tttatgatgg tgtcttgcat gacgtgttgg atcctttgtg
    26701 ctcgaacggg cattaaaccg cattatgttg gtggttattg cgagccgctt tccagaaaca
    26761 gaaaaaccat tacccctgaa aaccgaaaaa tgccacaata ttggctgttt atacagtatt
    26821 tcaggttttc tcatggcatt aaccgccgcg cttaaagcgc aaattgccgc ctggtataag
    26881 gcgcttcagg aacagatccc cgactttatt ccccgtgcgc cgcagcggca gatgattgcg
    26941 gacgtcgcca aaacgctggc cggagaagaa gggcggcatc tggcgattga agcccccacc
    27001 ggcgttggga aaacgctctc ctatttgatc cccggcatcg ccattgcccg cgaagagcaa
    27061 aaaacgctgg tggtgagtac cgccaacgtg gcgttgcagg atcagattta cagcaaagat
    27121 ttaccgctgc tgaaaaagat cattcccgat cttaaattca ctgccgcttt cgggcgtggg
    27181 cgctacgttt gtccacgtaa tctgacggcg ctcgccagca ctgaacccac gcaacaggat
    27241 ctgctggcgt ttcttgacga cgaactgacg ccaaataatc aggaagagca aaaacgttgt
    27301 gcgaagctga agggcgatct cgacacttat aaatgggatg gtctgcgcga tcatacggat
    27361 atcgctatag atgatgatct ctggcgtcgt ttgagtaccg acaaagccag ctgcctcaac
    27421 cgtaactgtt actactatcg tgaatgcccg ttttttgtta ctcgtcggga gattcaggaa
    27481 gcggaagtgg tggtggcaaa ccatgcgctg gtgatggcgg cgatggaaag cgaagccgta
    27541 ctgcctgacc cgaaaaatct gctgctggtg ctggacgaag gtcatcacct gccagatgtg
    27601 gcccgggatg cgctggagat gagcgctgaa atcaccgcgc cctggtatcg actacagctg
    27661 gacttgttca cgaaactggt cgctacctgc atggagcagt ttcgcccgaa gaccatccca
    27721 ccgctggcga tccctgaacg tttgaatgcg cattgtgaag agttgtatga gcttatcgcc
    27781 tcgttaaaca acattctcaa tctctacatg cctgccgggc aggaggcaga gcaccgtttt
    27841 gcgatgggcg aactgcctga tgaattgctg gagatctgcc agcggctggc aaaactcacc
    27901 gagatgctgc gtggtctggc ggagttattt cttaacgatt taagtgagaa aaccggcagc
    27961 cacgatattg tgcgtctgca tcggttgatt ttacagatga accgcgcgct ggggatgttt
    28021 gaggtgcaaa gcaaactctg gcggctggcg tctctggcgc aatcttccgg tgcgccggtg
    28081 accaaatggg cgacgcggga agagcgcgaa gggcagctac acctctggtt tcactgcgtg
    28141 ggaatacgtg tcagcgatca gctggaaagg ctgctgtggc gcagtattcc gcacattatt
    28201 gtcacctccg caaccttgcg ttcgctgaat agtttttcgc gtttgcagga gatgagtggt
    28261 ctgaaagaga aagcgggcga ccgttttgtg gcgctggatt ccccctttaa ccactgcgaa
    28321 cagggcaaaa ttgttattcc ccggatgcgc tttgagcctt ccatcgacaa cgaagagcaa
    28381 cacattgccg aaatggcggc ctttttccgt gaacaggtgg agagcaaaaa atatctcggt
    28441 atgttggtac tgtttgccag cggacgggcg atgcagcgct ttctcgacta tgtgacggat
    28501 ttacgcctga tgttgctggt gcagggcgat cagccgcgtt atcgcttagt tgaactgcac
    28561 cgcaaacgcg ttgccaacgg tgagcgtagc gtgctggtgg gcttacagtc atttgccgaa
    28621 gggcttgatt tgaaaggtga tctgctcagc caggtgcata tccacaaaat cgcttttccg
    28681 cccatcgaca gcccggtggt gatcaccgaa ggggaatggc tgaaaagcct caaccgctat
    28741 ccgtttgagg tgcaaagcct gccgagcgcc tcgtttaacc tgattcagca ggttgggcga
    28801 ctgattcgaa gccacggttg ctggggcgaa gtggttatct acgacaaacg cttgctgaca
    28861 aaaaattatg gcaagcgact actggatgca ttaccggtat ttccgataga gcaaccggaa
    28921 gtccctgaag gtatagttaa aaagaaagaa aaaacgaaat ccccacgccg tcggcggcgt
    28981 taatgatgtg agtcaggtaa ggagtcgtaa atggactatc gcaaaatcat taaagagatc
    29041 gggcgcggga aaaaccacgc gcgcgattta gaccgggata ctgcgcgcgg tctgtatgct
    29101 catatgctca atggtgaagt ccctgacctt gagttgggcg gcgtattgat tgcgctgcgt
    29161 atcaaagggg aaggggaagc agagatgctc ggcttttacg aagccatgca aaatcacacc
    29221 atcaagctga cgccgcctgc aggcaagccg atgccgattg tcatccccag ttacaacggc
    29281 gcgcgtaaac aggctaacct gacgccatta ttggcgattc tgctgcataa actcggtttt
    29341 cctgtggtgg ttcacggagt tagcgaagat ccaactcgcg tgctgacaga aaccattttc
    29401 gaactgatgg gtattacacc aacgttgcac ggcgggcagg cgcaggcgaa gctcgacgaa
    29461 catcaaccgg tgtttatgcc tgtcggcgcg ttttgcccgc cgctggaaaa acaactggcg
    29521 atgcgctggc ggatgggcgt gcgtaacagt gcacataccc tggcgaaact ggcgacgcca
    29581 tttgccgaag gtgaggcact gtgtctttcc agcgtttcgc atccggaata cattggacgc
    29641 gtcgcgaagt tctttagcga tatcggcggg tgggcgttac taatgcatgg tactgaaggc
    29701 gaagtgtatg ctaatccgca gcgctgtccg cagatcaatc tcattgaccg tgaagggatg
    29761 cgggtgctgt atgaaaaaca ggaccctgct ggtagcgagt tactgccaca agcaaaagat
    29821 ccggaaacca cggcgcagtg gactgagcgt tgcctggctg gcagcgaagc gattcccgaa
    29881 tcgctgaaaa tccagatggc ttgctgcctg gtggctacgg gtgaagcggc aactatcagc
    29941 gatggcctgg cgcgcgttaa tcaggcattt taatttttat ctcagccgga tgagccacgt
    30001 catccggctt ttaccccacc gcaatatgaa attcctgcat ctttattgac cttcccgcgc
    30061 ccggcgtgca gcataaaaaa acaacaaaca cataacataa acaggagtta accatggaaa
    30121 gtggtcatcg ctttgatgct cagacgctgc acagttttat tcaggctgta tttcgtcaga
    30181 tgggtagcga ggaacaagaa gcgaaattag ttgccgatca tttaatcgcg gcaaacctgg
    30241 cagggcatga ttctcatggt attggcatga tcccaagcta tgtgcgttcc tggagtcagg
    30301 ggcatctgca aattaaccat catgccaaaa tcgttaaaga ggcgggggcg gcagtcacgc
    30361 tcgatggcga tcgcgcattc ggtcaggtcg cggcacacga agcgatggcg ctggggattg
    30421 agaaagcgca tcagcacggt attgctgccg tggcgctaca taactcgcat catatcggcc
    30481 gtatcggtta ctgggcggag cagtgttcag cggcggggtt tgtctctatc cactttgtta
    30541 gcgtggtcgg tattccaatg gtcgcgccgt tccacggtcg cgacagccgc tttggcacca
    30601 atcccttctg tgtggttttc cctcgtaaag ataatttccc gctgttgctt gattacgcca
    30661 ccagcgccat tgcatttggc aaaacccgcg tcgcctggca taaaggcgtc cccgtgccgc
    30721 caggttgcct gattgacgtt aacggcgtgc cgacgaccaa tccggcggta atgcaggagt
    30781 cgccgttggg gtcgctgttg acctttgccg aacataaagg ctacgccctt gcagcgatgt
    30841 gtgaaattct tggcggggcg ctttctggcg gtaaaacgac gcatcaggaa acgttgcaaa
    30901 ccagccccga tgccattctt aactgcatga ccactatcat catcaacccg gaactgttcg
    30961 gcgcgccgga ttgtaacgcg cagaccgaag cctttgccga gtgggtgaaa gcctcgccgc
    31021 atgatgacga taagccgatt ttgctaccgg gcgagtggga agtgaacacg cgtcgcgaac
    31081 ggcaggagca ggggattcca ttggatgcgg gaagctggca ggccatttgt gatgcggcgc
    31141 ggcagattgg tatgtcggaa gagacgttgc aggctttctg tcagcagtta gccagctaaa
    31201 aaaaagcccg tccagtggcg gacgggcaaa caagggtaac ataggatcaa tgagggttag
    31261 agcatatgcg tctgtcggca aacagacagg gaaatacttg tgctggacgt agcgtaaacg
    31321 cctgatccgt ccaacggttc ggattttgta ggcctgataa gacgcggtag cgtcgcatca
    31381 ggcatctgtt gccggatgcg gcataaacgc cttatccgtc ctacgaatta cttataaatt
    31441 accgcagtac cgcttaattt attgttgttg gtggcggaag tgatgctgta tccactggca
    31501 ccggctgccg ccgctttctc agccagtttc gcttccaggg catcgagggt ggatgcgcca
    31561 tcggcagaaa ccacgccgat tttattcatg ttctgtgcct gggatgccgt taccggttcc
    31621 gccgcgaaca cgccaaatga cagagttgaa agagccatag cagcaacaac agtattgata
    31681 gttttcataa ttaatctctc gcaggtgatt tttgtataag tgacgttgtt tcgtcgatgt
    31741 gatgagtatc acgttttttt acgagagata aaatcgaaga gaattgaccg ccttgttcaa
    31801 ataaattgat tgatatctaa catactgata aaccatttat ttgttgtact tatggacttg
    31861 ttttatgccg cgtggcagtc acagcagcgt taaaggcacg gaatgccacg caaaatacaa
    31921 attatactcg ctatgcatct cgccagttgt agttaacgta actgtctgtc tttcgaacct
    31981 ctgcgattat atcctgtata cgctggtttt tgtaaatctt tctcctgctg acaatgaata
    32041 cataagcgca cgccaggaat ggcttcccga cgggcttgcg ggatgggggc accgcactct
    32101 tcacattcat acaggctttc gccacgcgga atttcacccc gagcgcgggc aatcgcatct
    32161 tcaattgtac tgttgatctg ttcgttgacg gcgtcatcgt tagcccaagc ggatgccata
    32221 tcgtcctccc catatcaatg cttgtacagt taagtgtagc taatataggg acgaacctgg
    32281 gcagttcaag cgtcagatct ccgaccattc ccgcagcaga ttatgataaa gattgagcag
    32341 cgacaggatc tcttcatttt caccatagcg gcttttcagc gactgaatat ttttgtccag
    32401 ttcaaacagc atggcgcgct ttttatcatc gcggatcatc gactggatcc acataaatga
    32461 tgccactcgt acgccgcggg ttacgggtgt cacgcaatgc aggctgctgg agggatacaa
    32521 caccagatca cctgccggga gttttacccg atgttgtcca aaggtgtcat taacgaccag
    32581 ttcgccgccg tcgtagcttt gtggatcgct taaaaacagc gtggcagaaa ggtcagtgcg
    32641 catccagccg ttttgtggat ggctgcgtac tgcgccatcc acatggaaac cataggtttc
    32701 attgttctga tagcgattaa acagcggcgt ggaaagggta cgcggcaagg cagcggcaaa
    32761 gaacaatgca tgttggttaa ccgcgttcag cacctcattt tgcagtgcgg cgtataacgc
    32821 gctgcgggtg tctacttgct gattgttctt aacttgtgca ccttgtgctc cggtggtgac
    32881 gcgtccatcc acccattcgg cttgttccag ttgttcgcga aaatgggcga cgtcctgtgg
    32941 cgataacacg ccgggaatgt ggtacatcat acaagttctc caaaaagtgg ggcctgcgcc
    33001 ccacatctga atcagaaatg catattggct gtgagcaaga aggttcttgg ctcgcccggg
    33061 tgataacggt agccgctctt gttgattgag gcgacgtaat cggtatcaaa caggttgtaa
    33121 acgtttagct ggaagtcgag attgcgatta acccgatacc ccagtttggc atcggcgacc
    33181 cagtaacctt ctgtaaatgc tggcgttccc accgcgccgt ctgaaccttt atgtatactg
    33241 ccgatatagc gtgcgcccgc gccaacagag atatcatcgg ttgcctgata ttggctccat
    33301 aaggtgaagg cgtgctccgg ggtatacggc agcgatgagg aaccatcctg ggcaacatct
    33361 ttgccgtttt tgatggttgc tttttgctgg gtatagccgc caatcacctg ccacgcggga
    33421 gtgatattcc ctgccacgga tatctcatag ccttcgacgc gtttcttacc gtattgcgag
    33481 taagtcccgt catcattttg ctcaacttca ttttcgatat cagtacggaa cagcgcggcg
    33541 gtgagcaaca ggcgtttatc cagaacctgc catttggtgc caatctcgct ggtgttggct
    33601 ttttgcggtt taaaatcggt gcggttggca ctgttaccac tgccagactg cgcaagggcg
    33661 aagttgttgc cgcccggagg ctgctgggaa acggcatagt taatatagac attgccgttt
    33721 tccgtcaggt ggtacagcgc cccggctttc cagttcacca gattgcccga cttggcggtg
    33781 tcgacggtgg tgactggaga accttttgcc acaccagctg ggcaggtgat ggcaccgcgt
    33841 ccgctgccgc cgcaggcggt ggcactgtca tattcagtat gataattatc cagacggatc
    33901 ccgccgttca gctcaaaatc acgggtgatt tgcagcgtat cgaaggcgta aattgcgaag
    33961 gtatccgtct gaccattggc gtttgcgccg ttgcgcgtca ggccgccggg atgaattctg
    34021 ctgtcaggat gataaatatt taccgccggt aacgtcaccg gattaacgcc atagttagtc
    34081 tgcgtttcac gggtaaattc cacgccggta ctgacatcat gaccgataga accggtatag
    34141 aacgtcgagg tcaggttggt ctggttggtg agaattttat tactcacatc tttggtattc
    34201 gccgtgcgtg actaggtcca gctattcaca tcgctggtgg gctgagtaat attcgacgcc
    34261 ccgcccataa tcgccgtcat caggtaatcc tgctttacgc gcgaccaacg ggtagtattg
    34321 cgaatggtgg tgttatcgtt gatgtcgtgc tcaaaacgca tggtggcggt gtcggtggtc
    34381 gaatcgtcgt aatcggaatc cgtgccgtaa aagttatgag tatcaacttt tccggaatga
    34441 ttcagggccg ccgctcccgc agatggggca gaatagcccg gcaaaccgat ggtcggaatg
    34501 ccgccgtctg gcgtgttgtg ctgagtgaca tgcagataat taagatacaa acgatttgct
    34561 gtaccaaggc caaaagcgac agaaggggcg acgccgtaac gctcattttt gactttgtcg
    34621 cgtccggcat cgtgggtttt ttcgcccatc acattcaggc gcaccgcggt agtatcacca
    34681 atgacctgat tgacgtccag cgtgccgcgg cggaaccagg cgctgccaat actggcggag
    34741 gcgtcaatgc cggaatcatt gcgcggctgc ttgctgatca tattgatcga gcctgtcggt
    34801 gcgctgcgcc cgtagtcggt gccggacggc cctttaatca cttcgacctg ctcggtattg
    34861 aaggtgtcgc gcgagacgct gccgatatcg cgaataccat caatataaat actgttagag
    34921 gtatcggcac cacgcatata aatggcgtcg ccagtggtgg agttaccgtt ctcacccgca
    34981 aaaaacgcac ccacgccgg
//
LOCUS       DAJTQY010000032        34939 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.032, whole genome shotgun sequence.
ACCESSION   DAJTQY010000032 DAJTQY010000000
VERSION     DAJTQY010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34939
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.032"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            <1..2261
                     /locus_tag="OO349_001675"
     CDS             <1..2261
                     /locus_tag="OO349_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HCR5866789.1"
                     /translation="AKSLYLNPDKNTVNAFIHQQDINVLAQDDYQSLSIQGNGALGIT
                     ENSYIGAHWNFNGYDADDVSDSNTDVSDLYYRYDFLRRYYVQAGRMDNRTLFNAQGGN
                     FTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGTPVMVLLSRNSRVDAYRNEQLLGSFYL
                     NSGSQFIDTSSFPPGSYSVALKVYENNQLTRTELVPFTKTGGLTDGNAQWFLQAGKTT
                     SQVSDDESSAYQLGVRLPLHPQYELYAGLANADDVSAFELGNNWTADLGRVGNLAISA
                     SVFRNDDGGKGDMQQANWSNPGWPTLGFYRTNSDGDACTTDSRESYNALSCYESISAT
                     VSLNFVGWNMMLGYTCTQNNTDDSLRWDKQQSFENNYLRQTTAQSISETVQLSASRAI
                     VMRDWILSTSVGVFHRNDNGGDNDDNGLYLSFSLSDTPTMDSNNNSHSTNVPTDYRYS
                     EQDGDQTSWQLSHTFYNDSFSHKELGVTVGGLNTDTINSAVNGRWDGQYGNVYATVSD
                     SYDRKNHDHLSAFTGTYSSTLAVSRYGVNLGASGTDDLLGAVLVDVKGFSEQDEESQD
                     LQLEARVAGSRTLQLGQSDSVLFPYPGFQSGFVEVNDSSQGNQQGTTNIINGAGNREL
                     MLLPGKLRYREVSASFNYNYIGRLLLPAAVKKFPIVGLNSAMLLVAEDGGFTLEINGS
                     EKELYLLSGQQFLKCPLSVVKKRASIRYSGDVTCSVVTYSQLPESIQVQAQLKQPKLR
                     GNVQTAQREVAP"
     gene            2258..3346
                     /locus_tag="OO349_001676"
     CDS             2258..3346
                     /locus_tag="OO349_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CblD"
                     /protein_id="HCR5866790.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPLGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGYFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(3434..4732)
                     /gene="exuT"
                     /locus_tag="OO349_001677"
     CDS             complement(3434..4732)
                     /gene="exuT"
                     /locus_tag="OO349_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HCR5866791.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            5216..6628
                     /gene="uxaC"
                     /locus_tag="OO349_001678"
     CDS             5216..6628
                     /gene="uxaC"
                     /locus_tag="OO349_001678"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HCR5866792.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            6643..8130
                     /gene="uxaA"
                     /locus_tag="OO349_001679"
     CDS             6643..8130
                     /gene="uxaA"
                     /locus_tag="OO349_001679"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HCR5866793.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADPEVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            8213..8764
                     /locus_tag="OO349_001680"
     CDS             8213..8764
                     /locus_tag="OO349_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HCR5866794.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAIDERG"
     gene            complement(8769..10013)
                     /gene="sstT"
                     /locus_tag="OO349_001681"
     CDS             complement(8769..10013)
                     /gene="sstT"
                     /locus_tag="OO349_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HCR5866795.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPFGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(10609..11574)
                     /gene="alx"
                     /locus_tag="OO349_001682"
     CDS             complement(10609..11574)
                     /gene="alx"
                     /locus_tag="OO349_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HCR5866796.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            complement(11845..12843)
                     /locus_tag="OO349_001683"
     CDS             complement(11845..12843)
                     /locus_tag="OO349_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005050984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR5866797.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNSLHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKAGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLGSEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKLYLPK"
     gene            complement(12922..13614)
                     /locus_tag="OO349_001684"
     CDS             complement(12922..13614)
                     /locus_tag="OO349_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HCR5866798.1"
                     /translation="MLRAFARLLLRICFSRRTLKIACLLLLVAGATIFIADRVMVNAS
                     KQLTWGDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRA
                     IWLAKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMI
                     GPFSEHGCPAKE"
     gene            complement(13687..14190)
                     /locus_tag="OO349_001685"
     CDS             complement(13687..14190)
                     /locus_tag="OO349_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HCR5866799.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            14275..15411
                     /gene="rlmG"
                     /locus_tag="OO349_001686"
     CDS             14275..15411
                     /gene="rlmG"
                     /locus_tag="OO349_001686"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HCR5866800.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     PGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHYARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            15696..16010
                     /gene="higB"
                     /locus_tag="OO349_001687"
     CDS             15696..16010
                     /gene="higB"
                     /locus_tag="OO349_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HCR5866801.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            16007..16423
                     /gene="higA"
                     /locus_tag="OO349_001688"
     CDS             16007..16423
                     /gene="higA"
                     /locus_tag="OO349_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HCR5866802.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(16468..18486)
                     /gene="fadH"
                     /locus_tag="OO349_001689"
     CDS             complement(16468..18486)
                     /gene="fadH"
                     /locus_tag="OO349_001689"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HCR5866803.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRTVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADIVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQGCLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTILLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(18712..21063)
                     /gene="ygjK"
                     /locus_tag="OO349_001690"
     CDS             complement(18712..21063)
                     /gene="ygjK"
                     /locus_tag="OO349_001690"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HCR5866804.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVNFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKIISNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVNYNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            complement(21080..21499)
                     /locus_tag="OO349_001691"
     CDS             complement(21080..21499)
                     /locus_tag="OO349_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5866805.1"
                     /translation="MNGYIQYDLAEGITWMNGLEITDGTGQLYLTGLFTPNFAARAWH
                     HTGRADGLDVPGSESGMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIW
                     YEYGGGRFATAFDSRFYMKNASNDPSDQIFLMQYFYW"
     gene            complement(21477..21830)
                     /locus_tag="OO349_001692"
     CDS             complement(21477..21830)
                     /locus_tag="OO349_001692"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5866806.1"
                     /translation="MPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWW
                     GDFTPDLGYVIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYD
                     DKYTYPAMDERLHPV"
     gene            complement(21831..22151)
                     /locus_tag="OO349_001693"
     CDS             complement(21831..22151)
                     /locus_tag="OO349_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5866807.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGLGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNA"
     gene            complement(22285..23718)
                     /locus_tag="OO349_001694"
     CDS             complement(22285..23718)
                     /locus_tag="OO349_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5866808.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTVISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            complement(23781..24230)
                     /locus_tag="OO349_001695"
     CDS             complement(23781..24230)
                     /locus_tag="OO349_001695"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HCR5866809.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVENDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(24227..27304)
                     /gene="ebgA"
                     /locus_tag="OO349_001696"
     CDS             complement(24227..27304)
                     /gene="ebgA"
                     /locus_tag="OO349_001696"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HCR5866810.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SSQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDHGNVWYKFGGD
                     YPNNYNFCLDGLIYSDQTPGSGLKEYKQVIAPVKIHALTRGELKVENKLWFTTLDDYT
                     LHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSRTRYSE
                     AGHSIASYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKMSGKPT
                     SCQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQSDGEV
                     LIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFTMGING
                     EYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRWTALTN
                     RHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLGSNSWG
                     SEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ"
     gene            complement(27487..28470)
                     /gene="ebgR"
                     /locus_tag="OO349_001697"
     CDS             complement(27487..28470)
                     /gene="ebgR"
                     /locus_tag="OO349_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HCR5866811.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            28688..29019
                     /locus_tag="OO349_001698"
                     /pseudo
     CDS             28688..29019
                     /locus_tag="OO349_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417545.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
     gene            complement(29061..30440)
                     /gene="ygjG"
                     /locus_tag="OO349_001699"
     CDS             complement(29061..30440)
                     /gene="ygjG"
                     /locus_tag="OO349_001699"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HCR5866812.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            30858..32378
                     /gene="aer"
                     /locus_tag="OO349_001700"
                     /pseudo
     CDS             30858..32378
                     /gene="aer"
                     /locus_tag="OO349_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
     gene            complement(32532..33155)
                     /gene="nfeR"
                     /locus_tag="OO349_001701"
                     /gene_synonym="yqjI"
     CDS             complement(32532..33155)
                     /gene="nfeR"
                     /locus_tag="OO349_001701"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HCR5866813.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            33432..34177
                     /gene="nfeF"
                     /locus_tag="OO349_001702"
                     /pseudo
     CDS             33432..34177
                     /gene="nfeF"
                     /locus_tag="OO349_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417541.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
     gene            complement(34231..34306)
                     /locus_tag="OO349_001703"
     tRNA            complement(34231..34306)
                     /locus_tag="OO349_001703"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34271..34273),aa:Ile,seq:cat)
     gene            34431..34937
                     /gene="mug"
                     /locus_tag="OO349_001704"
                     /pseudo
     CDS             34431..34937
                     /gene="mug"
                     /locus_tag="OO349_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
BASE COUNT         8539 a         8973 c         9176 g         8251 t
ORIGIN      
        1 atgcgaagtc attatattta aatccagaca aaaatactgt taacgctttt atacatcagc
       61 aagacatcaa tgttctggca caggatgatt accaatcgtt gtctattcag ggaaacggtg
      121 cgctgggaat aacagaaaat agctatattg gtgcacactg gaatttcaac ggttatgatg
      181 cagatgatgt cagtgacagt aatactgacg tcagcgatct ctattatcgt tatgattttt
      241 tacgtcgtta ttatgtgcag gcgggtcgca tggacaaccg cacactattt aatgcacaag
      301 gcgggaactt tacctttaac tttctgccac tcggtgcaat cgatgggatg cgtatcgggt
      361 cgacactgag ctatttaaac caggcgcaaa gccagcaagg aaccccagta atggttctgc
      421 tttcgcgcaa ttctcgtgtt gacgcttatc gtaatgagca acttttggga tcgttttatc
      481 tcaatagtgg ttcgcaattt attgatacca gttcctttcc gccaggcagc tacagcgtgg
      541 cattaaaagt ctacgaaaat aaccaactca cccgcaccga gcttgtgccg tttaccaaaa
      601 ccggcggtct gactgacgga aatgcgcaat ggttcttaca ggcaggtaaa actacatcac
      661 aggtttctga tgatgaaagt tcagcttatc agctaggagt acgcctgcca ttacatccgc
      721 aatatgagct ctacgcaggg ctggcgaatg ccgatgatgt gagtgctttc gagttaggta
      781 ataactggac ggcagattta ggcagggtgg ggaatcttgc aatcagcgcc agcgtgttcc
      841 gtaacgatga cggcggcaaa ggtgatatgc aacaggccaa ctggagtaat ccgggatggc
      901 cgacgttggg cttttatcgg accaactctg acggcgatgc ttgtacaacc gacagcagag
      961 agagctataa cgccttaagc tgttatgaaa gtatttccgc gacagtttca ctgaattttg
     1021 tcggctggaa tatgatgctg ggttatacct gcacacaaaa taacactgat gatagtttgc
     1081 gttgggataa acagcagagc tttgaaaata actatcttcg ccagacaaca gcgcaaagta
     1141 tctctgaaac tgtacaactt agcgcttccc gcgctattgt gatgcgtgac tggattttga
     1201 gtacgtcagt cggtgttttc catcgtaatg acaacggtgg cgataacgac gacaacggct
     1261 tgtacttatc gttttcgtta tctgacacgc caacgatgga tagcaataac aacagccatt
     1321 caaccaatgt gcctacggat tatcgttata gcgaacagga tggcgatcaa acgtcatggc
     1381 agttatcgca taccttttat aacgattcat tcagccataa agaacttggc gtaaccgtcg
     1441 gaggcctgaa caccgatacc ataaacagcg cggttaacgg gcgttgggat gggcaatacg
     1501 gaaatgtcta cgctaccgta tctgatagtt atgaccgtaa gaatcatgat catctttcgg
     1561 cctttacggg cacttatagc tctacgctgg ctgtcagtcg ctatggcgtt aatttgggag
     1621 ccagtggtac agacgatttg ctgggtgcgg tactggtgga tgtgaaaggc ttctctgaac
     1681 aggatgaaga gagtcaggat ctgcaactcg aagcgcgggt ggcaggcagc cgaactttgc
     1741 agcttggtca aagtgacagt gtgttgttcc cttatcctgg atttcagtct ggttttgttg
     1801 aggttaacga cagtagccag ggcaatcagc aggggacaac aaacatcatt aacggtgcag
     1861 ggaatcgtga attaatgttg ttgcctggca agctgcgcta tcgcgaagtg tctgccagct
     1921 ttaactacaa ctatatcggt cgtttattac tgccggcagc ggtgaaaaaa ttcccgattg
     1981 tggggctgaa cagcgccatg ttactggtag ctgaagatgg cggatttaca cttgaaatta
     2041 acggcagcga aaaagagctc tatctgcttt ccgggcagca attccttaag tgtccactga
     2101 gtgttgtaaa gaaacgcgcc agcattcgtt acagcggaga tgttacttgt agtgtggtga
     2161 cttattcaca attaccggag tcgattcagg ttcaggcaca gttgaaacag cctaaattac
     2221 gtggaaacgt tcagacggcg caaagggagg ttgcaccatg agaaatcgat tgattgcggc
     2281 gatattgggc ttgtttggca cgctcactgg cgttcaggca gctcctgacg tgaccagtga
     2341 aattacgtat gatttggcat ctggtagagc ggattattac ttctggaagg atgaagcgtc
     2401 ggcaggaaat aatggatata tgtggtatga atgttcgtat cctgacctcc aacaaacctg
     2461 tacagctaat ggaaatatat cgacagtaca aatctattta actgaacaac gcagtgggat
     2521 gcgttggccg gtaaaactca aaggatttaa aacagccatt gtaagtagcg atgaagcgcc
     2581 gctaggatgc aaggggggca aagggcttca gacgaatctt aaggattcta atagatcttc
     2641 atgtacagaa gatggtcaac attattatat atacgataca aagtttctta cgctctacct
     2701 tgagcagaca gagatgaaga atttgccgat tggtggcgtc tggaagggga aagttaaatt
     2761 acattcgaac agcccggccc aggactattt cgcaaatatt accctgaata cgctcgaccc
     2821 caaccatatt gacgtgttct tcccggagtt cgcccacgcc acgccaaggg tgcagttaga
     2881 cttgcatcca acaggaagcg ttaacggcag caactacgcg caagatctga ccatgttgga
     2941 catgtgcctg tacgatggtt ttaacggtaa tgccatcagt tatgaaatca tgctcaaaga
     3001 tgaagggcga ccagctgcag ggcgcagaga cggttacttc tctatctatc gtcagggagg
     3061 gaccaccacc gacgagggag aacgcattga ttaccgggtc aaaatgtaca acccggaaac
     3121 cggtgggcaa attgatgtgc gcaataatga aaatatggtc tggaacagca ttaacctgaa
     3181 acgtgtgcgt ccggtggtac tgcccggtat tcgctatgcc gtgatgtgtg tgccgacacc
     3241 cttgactctg gcagtcgata aattcagcgt gatggataaa caggccgggt actacatggg
     3301 caaattgtca gtaatcttta cgccttcctt gccaaccatc aattaaccct gcaaaccgga
     3361 caaacgcttt tttctatata aacgacgaag ccgcctgata aggcggcttc gtcgttataa
     3421 cttacgttaa acgttaatgt tgcggtgcgg gatcgttatg tgtttcctgc gccacctcaa
     3481 tcgccggttt gttctgcaat acggtccaga taaccagcgc acccagcagg tcaaatactg
     3541 ccagaactgc gaacagcggg ctgaagccga tggtgtcagc cagtgcaccg acaaccagcg
     3601 caaacagcgt acttgccagc catgcggaca tcccggttaa accgttcgcc gtcgccactt
     3661 cgttacgacc gaacacgtca gaagagagcg taatcagcgc gccagacagt gcctggtggg
     3721 caaaaccacc gatacacagc agcataattg cgacatacgg gttggtgaac agaccgatca
     3781 tacccgggcc aatcatcagc acagcaccca gcgttacgac catcttacgg gaaacgatca
     3841 ggttcacacc aaaccaacgc tggaacagcg gcggcaggta accaccgagg atacaaccga
     3901 ggtcagcaaa cagcatcggc atccaggcga acatcgcgat ctctttcagg ttaaagccgt
     3961 aaactttaaa catgaacagc gggatccacg cgttaaaagt accccaggcc ggttctgcaa
     4021 ggaaacgcgg cagtgcgata ccccagaact gacggttacg caggatctga ccaacggaca
     4081 ttttcttcgc cgtgctcacc tggtgctggg cttcctgacc attaataata tagtcgcgtt
     4141 cttcatcggt cagatgcttc tggtcgcgcg gatgtttata gaaaatcagc catgccatcg
     4201 cccagataaa gctcaatgca ccggagatga taaatgccat ctgccagctg tgcatcacga
     4261 ttgcccatac caccagcggc ggcgcaatca tcgcaccaat cgaagaacct acgttaaagt
     4321 agcctactgc gatggaacgc tctttcgccg ggaaccattc ggagctggct ttcagacccg
     4381 ccgggatcat cgctgcttcc gcggcaccga ccgcaccacg agcaacagct aggccacccc
     4441 agctacctgc cagcgcggtt gcaccacaga acacggccca cagcacagca aacattgcat
     4501 aaccgatttt cgtacccagc acatccagta cataacctgc taccggttgc atgaccgtat
     4561 aagcagcaga ataggctgcg atgatatagg aatactgttg ggtggagatg tttaactctt
     4621 ccatcagagt tggcgcagct gccgccacag tgttacgcgt caggtaacca agcacggtgc
     4681 cgagcgtcac cagtgcgatc atataccaac gtaacccttt aattttacgc atgtaaaacc
     4741 tcatcgtttt gttatatccg caggaagcgc ggccatcatc ccgccagagg ctgaagatgt
     4801 tagttacgac cgggtaaccg aaaaaaaaac cggcacgccc cgaacgttgc catgaaagtt
     4861 tcgtgctaat tcggcttccg taccggttaa cccgattcat ctaacaaatg aatgaatcgg
     4921 caatttgcat tccgtcgact tatgatttgc gacggcagaa agataacttg tcatacaacc
     4981 ttaaaaggtg agagccatca caaatgtgcg aatatttgta gggacattac ctgacgacag
     5041 cgaggccagt actggcgcgg cctgcagcga gatttaccac tttgagagta atttttttaa
     5101 ctacgtttat tgatctaact caagaaaata tcttcggact ctggaaattg gtgtgataac
     5161 tttgtcagca tcgcaccata agcaagctag ctcactcgtt gagaggaaga cgaaaatgac
     5221 tccgtttatg actgaagatt tcctgttaga taccgaattt gcccgccgtc tgtatcacga
     5281 ctacgcaaaa gaccagccga ttttcgatta ccattgccat ttgccgccgc agcagattgc
     5341 ggaagactat cgttttaaaa acctgtatga catctggttg aagggcgatc actacaaatg
     5401 gcgcgctatg cgtaccaacg gtgtggctga gcgtctgtgt actggtgatg cgtctgaccg
     5461 tgaaaaattt gacgcctggg cggcgactgt tccgcacact atcggcaacc cgttatacca
     5521 ctggacgcac ctcgaactgc gtcgtccgtt cggtatcact ggcaaattgc tttctccgtc
     5581 aactgccgat gaaatctgga acgaatgtaa cgaactgctg gcgcaggata acttctccgc
     5641 gcgcggcatc atgcagcaga tgaacgtgaa aatggtcggc accaccgatg acccgatcga
     5701 ttctctggag catcacgcag agatcgccaa agacggctct ttcaccatca aagtgctgcc
     5761 gagctggcgt ccggacaaag ccttcaatat cgaacaggcg acctttaacg actacatggc
     5821 gaagctgggc gaagtttccg ataccgacat tcgccgcttt gctgacctgc aaactgcctt
     5881 gaccaaacgt ctggatcact tcgccgctca cggctgtaaa gtgtctgacc atgcgctgga
     5941 cgtggtgatg tttgctgaag cgaacgaagc ggaactggac agcattctgg cgcgccgtct
     6001 ggctggcgaa accctgagcg agcacgaagt ggcacagttc aaaactgcgg tactggtgtt
     6061 ccttggtgcc gaatatgcac gtcgcggctg ggtacagcag taccacattg gcgcgctgcg
     6121 taataacaac ctgcgtcagt tcaaactgct ggggccggat gtaggctttg actccatcaa
     6181 cgaccgtccg atggcggaag agttgtctaa gctgctgagc aagcagaacg aagaaaacct
     6241 gctgccgaaa accattctct actgcctgaa cccgcgcgat aacgaagtgc tgggcaccat
     6301 gatcggtaac ttccagggcg aaggtatgcc gggcaaaatg cagttcggtt ccggctggtg
     6361 gtttaacgat cagaaagacg gtatggaacg tcagatgacc caactggcgc agctcggtct
     6421 gctgagccgc tttgtcggta tgctgactga cagccgtagc ttcctgtcat acacccgtca
     6481 cgaatacttc cgccgcattc tgtgccagat gatcggtcgc tgggtggaag cgggcgaagc
     6541 accggcggac atcaacctgc tgggcgagat ggtgaaaaat atttgcttta acaatgcgcg
     6601 tgactacttc gccattgaac tgaactaagg tctggggttg atatgcaata catcaagatc
     6661 catgcgctgg ataacgtcgc ggtcgcttta gcagatttgg ctgaaggcac agaagtcagt
     6721 gtcgataacc agactgttac gctgcgccag gatgttgctc gtggacataa atttgcgtta
     6781 acggatatcg caaaaggggc caacgtcatc aaatatggcc tgccgattgg ttatgcattg
     6841 gcggatattg cggcggggga acacgtccac gcccacaata cgcgcacgaa tctgagcgat
     6901 ctggatcagt atcgctatca acctgatttt caggatctgc ctgcgcaagc ggcagatcct
     6961 gaagtgcaga tctatcgtcg cgctaacggc gatgtcgggg tgcgtaacga gctgtggatc
     7021 ctgccaaccg tgggctgtgt caacggcatc gcgcggcaaa tccagaaccg tttcctgaaa
     7081 gagaccaaca acgccgaagg taccgacggc gtgttcctct tcagccacac ctatggctgc
     7141 tcacaactgg gcgacgatca catcaacacc cgcaccatgc tgcaaaacat ggtgcgccac
     7201 ccgaacgcgg gcgcagtgct ggtgattggt ctgggctgtg aaaacaacca ggttgccgca
     7261 ttccgtgaaa cgttgggcga tatcgatcct gaacgcgttc atttcatgat ctgccaacag
     7321 caggatgatg agatcgaagc cgggatcgag catttgcatc agctgtataa cgtgatgcgc
     7381 aacgataaac gcgagccagg caaactcagc gaactgaagt ttggtctgga gtgcggtggt
     7441 tctgacggtc tttctggtat tactgctaac ccgatgctgg ggcgtttctc tgactacgtg
     7501 attgctaacg gcggtactac cgtactgacc gaagtgccgg agatgtttgg cgcagagcag
     7561 ttgctgatgg accattgccg cgacgaagca acgtttgaaa aactggtcac catggtcaac
     7621 gacttcaaac agtactttat tgcccatgat cagccgatct acgaaaaccc atcaccgggg
     7681 aacaaagcgg gcggtatcac cacgctggaa gacaaatcac ttggctgtac ccagaaagcg
     7741 ggttccagtg tcgtggttga cgtgctgcgt tacggcgagc gtctgaaaac gccagggctg
     7801 aacttgttaa gtgcgccggg taacgatgcc gtagcgacca gcgccctggc gggtgcgggc
     7861 tgccatatgg tgctgttcag tactggtcgc ggcacgccat acggtggatt tgtgccgacg
     7921 gtgaaaatcg ccaccaacag tgaactggcg gcgaagaaaa aacactggat cgactttgac
     7981 gcgggtcagc tgatccacgg taaagcgatg ccgcagttgc tggaagaatt tatcgacacc
     8041 atcgttgagt ttgccaacgg taagcaaacc tgcaacgagc gtaacgactt ccgcgaactg
     8101 gcgatcttca aaagcggcgt aacgctataa agtcgaaatg agttgttaat aaaaaacggc
     8161 gtttcataat gtgaagcgcc gtttgctttt ccatacctgc taaggattta tcatgaccgc
     8221 gtattggctg gcccagggcg tgggtgtcat cgcctttctg attggtatca caacattttt
     8281 caatcgtgac gaacgtcgct tcaaaaagca gctttcggtc tatagcgccg ttattggcgt
     8341 acattttttt cttctgggca cctatcccgc tggtgccagt gccatcctta atgccattcg
     8401 tacattgatt accttacgca cgcgcagctt atgggtaatg gcgattttta ttgtgctgac
     8461 tggcggaatt ggcctcgcga agttccatca tcctgtcgaa ctattgccgg ttatcggtac
     8521 gattgtcagt acctgggcgc tgttccgctg taaagggctc accatgcgct gcgtgatgtg
     8581 gttttcaacg tgttgctggg tgattcacaa cttctgggcg gggtcgatag gcggcacgat
     8641 gattgagggt agttttctgc ttatgaatgg cctgaatatc attcgtttct ggcggatgca
     8701 gaaaagggga attgatccgt ttaaagtaga gaaaacccct cccgccatag acgaaagggg
     8761 ttaaacaatt aattacgcag ggcgctattt gccagacgat cgtcttccgc ctggcaagct
     8821 gccgcagtga acagcacgtc agttgaagag ttcagcgcgg tttcgcaaga gtcctgcaat
     8881 acgccgataa taaagccgac ggcaaccacc tgcatggcga tatcgttcga aataccgaac
     8941 atattacagg ccagtgggat cagcagcaaa gaaccccccg ccacgccgga tgcgccacag
     9001 gcacacagag aagccactac gctcaacagc agcgctgtgg gcagatcgac cggaataccc
     9061 agcgtattaa ccgcagccag cgtcaacacg gtaatggtga ttgctgcgcc cgccatattg
     9121 atggtggctc ccagcggaat agaaactgaa taggtatcgc gatccagatt cagcttttca
     9181 cacagcgcca tattcaccgg aatgttcgct gcagagctgc gggtgaagaa ggcatacaca
     9241 ccgctttcgc gcaggcacag cagcaccagc gggaacgggt tacgacgaat tttccaccac
     9301 accagcaatg ggttaaccac cagcgccacc agtaacatac agccaaccag cacgaccagc
     9361 agttgcgcgt agccccacag tgtggagaaa ccggtggttg ccagggtaga agaaaccagc
     9421 ccaaaaatac cgaacggtgc gaagcgaatg accagtttca ccataaaggt aacggcattc
     9481 gacatatcat tgaccaggtt tttggtggtc tcgttaccgt gacgcagtgc gaagccgagg
     9541 ccgatcgccc acaccagaat cccgatgtag ttacctttca gcagggcgtc gatggggttg
     9601 gaaaccatgc tcattaccag tccgcgcatc acttcgacaa tgcctgacgg cggcgaaata
     9661 tcacccgcgc tactggacaa gtgcagggta gaagggaagg caaagctgaa gactactgcg
     9721 gccagagctg cagagaaggt gcccagcaga tagaggaaca aaataggacg gatattggtt
     9781 ttctgcccgt gctggtggtt agcaatagat gccattacca gcatcaacac caggatgggg
     9841 gcaacagctt tcagtgcgcc gacgaacaaa gtacctaaca gaccaacagc ttccgccgcg
     9901 ggttttgaga tccatgccag aagaatcccc agaacaaggc cgaccaggat ttgttttacc
     9961 aggctgccat gagccagacg ccggaatagc cccggtgaac gttgcgtagt catttttcga
    10021 tcctttcatt gtgttgtcgc acatccctgg tgcgttctgt cgacgcatct ttcaggatgt
    10081 aacaaagtgt ttgcaaggtc gagtataagg aaagttacag cttcgggaag cgaaaaatgc
    10141 tggattttaa agagaggtca tgttttgcaa cttaatggtt gcatttattt aacataaaca
    10201 gagggaagga ctggctacta aggctgatta gataacatat gggcaaaact atttggaata
    10261 tgagaacggt tgcgctcctt taacgtgtta tgccatataa agtcactggc gcagggagcg
    10321 cgcagggggc ggccaatcgc cgccgccccc tgcaccccca ggctctggcg aacaaaatcg
    10381 ccgctgcgcg gttccctcag cctatccctt gcggctaccg ggtcgggcgc aaggtaacgt
    10441 ccctgtaaga tgcgccctcg ccccggcctc cagggaacgc ttcggcgatt ttgacgccac
    10501 caacacccgt gcggtctttt tgtaaagagc tataagccga ttacataatt aatagctcac
    10561 ctttgtctgg aatgagttat ctctaggaat ggcttaatat acagcgaatc attctccccg
    10621 ctgcttatca tgccgataat tcacccaggc gttgataata aacgtcatca ccagaatgcc
    10681 aaacaccacg cccagcgaga cggcgattgg aatatggtag aagtcgacaa tcagcatctt
    10741 gataccgata aacaccagaa tcaccgccag gccatatttg agcatcgaga aacgctctgc
    10801 tacgcccgcc agcaggaaat acatcgcacg caggccgagg attgcaaaca ggtttgaggt
    10861 cagcacaatg aacgggtcag tggtcacggc gaagatagcc ggaatgctat ccacggcaaa
    10921 aatcacgtcg ctcaattcca ccagaatcag taccagcatc agcggtgtgg cgtacagcaa
    10981 tccattctta cgcacaaaga aatgctcgtt gtcgatggtg tcggtcatgc gcaaatgacc
    11041 gcgtagccag cgcaccagcg gcttgtcgcc aatgcctgat tcatcttcat gggcgagcgc
    11101 catcttcacg ccggtaaaca gcaggaaggc accgaagata tacaggatcc agtcgaactg
    11161 cgaaatcagc cagctgcctg tgaagatcat gatggtacgc agaacaatcg ccccgagcac
    11221 gccatacacc agcacgcgac gttgtaatgc cgccggaacg gagaaatagc tgaacaacat
    11281 cagccagaca aagacgttat cgaccgccag cgatttctca atcagataac ccgtgagaaa
    11341 ggccagggcc tgtggatcgg caacggcgcg accttcggtt tgcaccagat accaccagaa
    11401 agcggcgtta aacagtaacg acagcgtcac ccagaccagc gaccaggccg cagcctgttt
    11461 catggtcatg gcatgtgccc cacgacgccc ctgcaacaac aggtcgatag ccagcataat
    11521 ggcgacaaca acagcgaatc cgccccatag caacggcgtg ccgacagtat tcatagaagt
    11581 tccttacaca taaaaaaacg gccaacgtcg gaagacgtca gccgctgctt tttatgcata
    11641 gacctcgcct tccggcaagg tctcacttac aacaaaaaag gaatacgtct gcgtattcct
    11701 ttcgggttgc ccggtgaccg gatgtggttt ttcacacatc gtaatgacga tcgaccggca
    11761 atgaagttac tcccctttgc aggtaacaaa gtatgacaga ccattcagtc agtcaatggt
    11821 ctgttacata ccttttacac tgttttactt tggcaagtat agttttacgc tatctgccgg
    11881 aaaaatcacc ccagtttggc ggcggatctc ggtcagcagt ttggctgtga tgcgactgac
    11941 cgccagccct ggatgatcca ccagatgctc atccaccagg gtagcgaaca gctctgcttc
    12001 gtagagcatg gtattaatat gctgcggctg ggtgagatcc tgcatctggc tgccacgcgg
    12061 cacaaagcag actttctggc attcagacag tttttcaatc accagcgatc ccgcttcgcc
    12121 ctgaatctcg cttcccagga cagaatcact gacctttgag tgctgcaagg tgacgctgaa
    12181 atcaccgtaa tccatcacca ccacgccgtg ggcgtcaacg ccgcttgcca gcaaactcgc
    12241 cgttgcctgc acgcttttcg gctcaccaaa taacgccacc gccgacgcca ggcagtaaaa
    12301 gccgatatcc ataattgaac cgttagagaa tgccggatta aaggtgttgg gattctcacc
    12361 gtccaggtaa cgttgatacc gcgaggaata ctggcaatag ttgaaaaaga ctttacgcag
    12421 tttgcctgct ttcggcagcg cctggcgcaa caaatgaaag ttcggcaggc aggcggtttt
    12481 aaatgcctca aacagcacca cctgattttc gcgcgcacag gcaatggcgg catccacttc
    12541 cgccagattc gacgccagcg gtttttcgca aatcacatga attttatggc taaggaaaag
    12601 ttgtgtctgg gaaaagtgca gggaattcgg gctggcaata tacaccgcgt caatggcatc
    12661 gctttccgcc atcgcttcca gcgacgtaaa cagatgctcg acagaaaaat cattggcgaa
    12721 gtgctgagcc tgctcaaggc tgcgggaata tacggcggtt aacttgtatt taccgctctc
    12781 atgggcggcc tcgacgaact ggcgagtgat ccagttcgta ccaatcacag cgaaacgtat
    12841 cataacgcgt ggatactcca taaagggatt ctgtcgtcaa tgtagcacgc ctttttcatc
    12901 ttcgacgtgc gcagatacgc attattcttt cgccggacag ccatgctcac taaacggccc
    12961 aatcatgacc gatggcccga gatatttcgg ctggcgatgg aggatttttg cgtcaatcac
    13021 cgcgctgaca cgagcgagtt tctcccgcag ttgagtataa aaaccgtgct tcatattgag
    13081 gtctggcgcg ttaaacccga tagcgtcgat gccgtactgt tttgccagcc agatggcgcg
    13141 ttggttatga aattcttgcg agataatggt gatatggttt tccccaaaca cctttttggc
    13201 acgcactacc gaatccagcg ttgagaatcc ggcatagtcg caaaagatca ctttcgccgg
    13261 tacgccttta gcgatcaaag cctgctgcat acctgatgct tcatcgtaat ttttacgtcc
    13321 gttatcgcca ctcaccagta gccatttcac ttttccggcg tggtacaacg ctgctgcggt
    13381 atcaatacgt cgggtaaagt agcgattacc cggcctcgcc cccagcaata aacccacgtt
    13441 acgcgccggg acggcgttga catcgcccca ggtcagctgc ttgctggcgt tgaccataac
    13501 gcgatcggcg ataaaaatgg tcgctcctgc cacaagtaac agtaagcagg caatctttag
    13561 agtccggcga gaaaaacaaa tacggagaag aaggcgggca aatgcgcgca gcatggtcgt
    13621 tatccttaac gaaagggggg aggtcgattt taggcgctaa agaaaagata acgcctgaca
    13681 tcattttcag attttgtcct gcccaagcga caactgcgtt agccacagcc gggtgtcgaa
    13741 ctcaagctgg tggtactgcg gctccatatg gcagcacaat tgatagaacg ctttgttatg
    13801 ctctttctcc ttcaggtgcg ccagctcgtg caccacgatc atgcgcaaaa acggttccgg
    13861 cgcgttgcga aacacggtag cgacgcggat ctctgctttt gctttcagct tgccgccctg
    13921 cacacgagaa acagcggtat gtaacccgag cgcgtttttt agcacatgga tcttattgtc
    13981 atacatcact ttattgatcg gcggggcatt acgcagaaac tgatttttca gatcctgagt
    14041 atattgccag agagctttat cggtggcgta gtcgtgtgtt cccggatagc gttttgccag
    14101 cacatcgccc agacgctgtt cgttaatcag tgttcgcacc tgcgaaagca gctgctcggg
    14161 ataaccctga agataagtaa gattgctcat aaccgcccac gaaaaagaga aaaagggtat
    14221 actcacgcac ccttttcagg ggcaacgtcg aaattttatc attcaggagg gccgatgagc
    14281 cacttagaca acggtttccg ttcactgaca ctacaacgtt ttccggcgac ggatgacgtt
    14341 aacccgctac aggcgtggga agcggcagat gaatatttgc tgcaacagtt ggacgacaca
    14401 gaaatccccg gcccggtgtt gatcctgaat gatgcctttg gtgcgttaag ctgcgcgctg
    14461 gcggaacata agccgtacag cattggcgac tcatacatca gtgaactggc gacgcgcgag
    14521 aatttacgcc taaacgggat tgatgagtcg agcgtgaagt ttctcgacag caccgccgac
    14581 tacccgcaac agccgggtgt ggtgctgatc aaagtgccga aaacactggc attgctggaa
    14641 cagcaactgc gtgcgctgcg caaagtggtc acgtcggata cacgtattat tgccggtgcc
    14701 aaagcccgtg acattcacac ttccacgctg gaactgttcg aaaaagtgct cggcccgacc
    14761 accaccacgc tggcatggaa aaaagcgcgc ctgattaatt gcacgttcaa tgaaccgccg
    14821 ctggccgatg cgccgcagac cgttagctgg aagctggaag gtactgactg gactatccat
    14881 aaccatgcga atgtcttctc ccgcaccggg cttgatatcg gcgcgcgctt ctttatgcaa
    14941 catctgccag agaatctcga aggggagatt gtcgatctcg gttgcggtaa tggcgttatt
    15001 ggtctgacgc tgcttgataa aaacccgcag gcgaaagtgg tgtttgtcga tgaatcgccg
    15061 atggcggttg cttccagccg tttgaacgtt gaaaccaaca tgccagaggc gttggatcgc
    15121 agcgagttta tgatcaacaa cgcgctctcc ggcgtggagc ctttccgctt taatgctgtg
    15181 ctctgcaacc ctccgtttca ccaacaacat gcgctgaccg ataacgtcgc ctgggagatg
    15241 ttccactacg cccgccgctg cctgaaaatc aacggcgagc tgtatatcgt tgccaaccgc
    15301 catctggatt acttccataa actgaagaaa attttcggca actgcaccac tattgcgacg
    15361 aataataaat ttgtggtgct gaaagcggtg aagctgggac gtcgtcggta agattgggag
    15421 ttacctgccg gatgcggcgt aaacgcctta tccggcctac gttcagcacg ttacccctag
    15481 gccggataag acgcggcaag cgtcgcatcc ggcaatgtct gctcagttct ggaatcgcgc
    15541 ttccataaaa caaaaaatcc ctgtcaccat cctctacatt ctcttgttta gcgtttttct
    15601 acgtttattc ttccgtcaca cagataaatt catccgttgc caatctgtca acggatgtta
    15661 tcatattgcc aatttgtcaa cggacgtgat gaagaatgca cctgataact caaaaagcat
    15721 tgaaagatgc tgcggaaaaa tacccgcaac ataaaacgga gttggtggct ctggggaaca
    15781 cgattgctaa gggatatttc aaaaaacctg agtcattaaa agcagtattc ccatctctgg
    15841 ataacttcaa atatctggat aagcattatg ttttcaatgt tgggggcaat gaattacgtg
    15901 ttgtagcaat ggtctttttt gaatcgcaaa agtgctacat acgtgaagtt atgacgcata
    15961 aagaatacga tttctttacc gctgttcatc gtactaaggg gaaaaaatga ttgcgattgc
    16021 cgacatcttg caagcaggag aaaagctaac tgctgtggca ccttttctgg cgggtattca
    16081 gaacgaggaa caatacaccc aggcgctgga actggtagat catctgctgc tcaacgatcc
    16141 tgaaaacccc ttgctggatc tggtgtgtgc caaaataacc gcgtgggaag aatcagcgcc
    16201 cgaatttgcg gaatttaatg ccatggctca agccatgcct ggcggtatag ccgtgattcg
    16261 tacccttatg gatcaatatg gtttaaccct ttccgatctg ccggaaattg gcagtaaatc
    16321 tatggtgtca cgcgttttga gcgggaagag gaaattaacg ctggaacacg ctaaaaaatt
    16381 ggcaacgcga ttcggcattt ctcccgcctt gtttattgat taacgcgacg tgccggatgc
    16441 gttgcatccg gcgcttcaaa taacgattta aatctccagc gccagccgtg ttccctgggc
    16501 aattgcccgt cgtgcgtcca gctccatagc cacatcgcag ccgccaatta aatgcacagt
    16561 tttcccgcta tcaatcagcg gttgcgccag cgcgcggttt ggctcttgcc ctgcgcagat
    16621 caccacattg tccactgcta atacctgcgt ttcgccgttg atcaccacat gcagcccgtc
    16681 atcgtcaatc ttctgataac ttacgcctgg gatcattttc acaccccgcg agagcaggat
    16741 ggtgcgatgg atccagccgg tggttttgcc taacccctgt cccggtttgc tggctttgcg
    16801 ctggagcatc acaatttgcc gtgggctacg cgggatctgc attccctgcg ggcttaagcc
    16861 accagcctgt tgtaggctgc tgtcgatccc ccactcatta cagaacctgg cgatattctg
    16921 gctggtggat tcgcccggct gacttaaata catcgccgta tcaaaaccaa tcccgccaca
    16981 accgatgatg gcaaccttgt tgcctaccgg cgctttgtcg cgcagtacat cgagataact
    17041 caataccttc ggatgatcga tcccgtcgat aggcggaaca cgcggcacga tcccactggc
    17101 gaggatcgtt tcatcgaacg cctgtaactg atccgccgtc acggtgtgat tgagtttcag
    17161 cgtcacgccc gtcacttcga tcatccggcg gtagtagcgc agcgtttcgt aaaactcctc
    17221 tttgccgggg atctgtttgg cgatattaaa ctgcccgccg atctcgctgt gcgcatcaaa
    17281 caatgttacc tgatgcccac gcgccgccgc gttaatggca aacgccagcc cagcaggtcc
    17341 agcgccgacc accgccagat tttttttctg cacggcggga aggattggca ttttggtttc
    17401 gtggcaggcg cgaggattca ccaggcacga ggtgactttg ccaacgaaga tctgatcgag
    17461 gcagccctgg ttgcagccaa tacaagtgtt gatctcatcg gctcgccctg attgcgcttt
    17521 tgacagcagt tccgcatccg cgagaaacgg tcgcgccatc gatactatat cggcatcgcc
    17581 gcgcgagaga atatcgtcgg caacctgcgg atcgttaatc cggttggtgg ttaccagcgg
    17641 cagcgagacg tggcctttca gtttgcgcgt gacccagcta aatgcgccgc gcggcacggg
    17701 cgtggcgatg gtcggaatcc gcgcttcgtg ccagcctatt ccggtgttga taatggtcgc
    17761 gcccgccgct tcaatggcct gcgccagttc taccgtttcg gcaaaagtgc cgccgccttc
    17821 gaccaggtcg agcatcgaca accggtaaat aataatgaag tcgttgccga cgcgttcgcg
    17881 caccgtacgc actacttcta cggcaaatcg catccggttg cggtaatcgc cgccccactg
    17941 gtcactacgc tgattggtgc gcagcgtcag aaattcgttg atcaaatacc cttcagagcc
    18001 catcacttcg acgccatcgt atccggcctc ccgcgccagt tgcgcgcagc gggcgaaatc
    18061 gtcgatcagt tgcaggatct cttcatgggt gagttcgtga ggaacgaaac ggttgatggg
    18121 ggcctgcaat gcggacggtg cgaccagatg cggttggtag ctgtagcgcc cggtatgcaa
    18181 aatttgcagg gcgattttgc cgccttcctg atgtacggct tcggtaatgg tgcgatggtg
    18241 tgggatctgg ctggcgtcgt tgagcattgc gccaccttcc atgccaacgc ctgttaaatc
    18301 tggtgcaatg ccgccgctga caatcagcgc cacgccgtga cgggcgcgtt cggcataaaa
    18361 cgcagccagc cgttcagcac cgtccgggta ttcctccagc ccggtgtgca ttgaacccat
    18421 caacacgcgg ttttttaacg tggtaaaacc taaatccagc ggggcgaaca gcgacgggta
    18481 gctcataaaa ttgtccagta tgtaaaataa ttgttatgta gtcggatgag ttctaattta
    18541 gccgtcgatg gagaaaaggg aaaaggggag tgcggggttt gtgatgggat tcaaaaagaa
    18601 tacgcccggt ggtaacgccg gatgcgacgc ttgccgtgtt ttatccggct tgggggattg
    18661 cacatgtagg gcggataagg cgttcacgcc gcatccgcca gccgttgcaa attactgctt
    18721 acggaaaaaa tcgttataca acatatacaa atgagcggca ctccaggaga aatttggtgc
    18781 gccttgctgc gcgcctgtca gcgggttgta attctcctga attgggccat cggcggttaa
    18841 tcctttggcg tgcctgaaga acgtatccgc cagcttcagg gcatcatcac gataaccgta
    18901 acgctccatc cctttcagac caaaccagaa ctgatccacc catacgcgcc cgcgccagta
    18961 gatatcagcg ccaaaagccg gattggttaa cgccgccgtt cccagcggaa caaaggtgtt
    19021 gaactcttta ggatcgagca tcaccttcac caccgcgtcg gcattggcct gcgttgccgc
    19081 accgttaaac agcggcgacc agccttccgg ccctttaccg cgctcaacaa tcggtttgcc
    19141 cgcgcagccg ttcgccagtg gtttatcttc aatacgtacg tcatagtaga actgggtagt
    19201 cgggtcgaac atacaggtgt tgatgtagtc cgcgagctgc tgtgccaact ggcgatagcg
    19261 tttggcctct tctggcttac cgaggatggt tgccatctcc gccagataat ggttatcgct
    19321 gtacatatag ctggcctgat ccaccgactc ctgcaatagc gagtagccca gcaacgttcc
    19381 gtcctgactg cggttttcgg cgaatttcac cgtccagtcg ctacgtttgc cgccattagc
    19441 gacatattta tccaactgtt ctttgtcgat aaaaccaaag acggcggcat catcacggcc
    19501 cgattcccac gacgcagcga cctgtgccgg aatttccaga ctgtcgtact ggcctttctc
    19561 caccacgcgg gcgtagttgt tcagcccaga ctgcgtctct tctttatcgc cttttttcac
    19621 cgtaaacagc atctcgccgc tctcggtgtt gtgggctttg tcgcgggtcg cgccatattc
    19681 cggcaccccg ttgccgttat gatcgcggtt acgtaaccac cagtcgtgat aggccaccag
    19741 tttcgggtac atctctgcca accaggcttt atcctgggtg acgttgtata cttccatcac
    19801 cgaccaggcg gcaaggctgg gtttggtatt acgttcgttc cagttgccgc catcgccacc
    19861 acgctcgggg ctaaggttcc acgctatcag gtcggggaca aagcccacat cctgcggacg
    19921 cacgctgtcg ccaggctgga tctgccagga gaagaccgcg cggatattct ctttggcgat
    19981 atccggattg aaatgcgcca tcgcaaacgc ctgcttccag gtatcccacg gccaggtctg
    20041 attgccggag aaccagcgtc cggtcaccga cggcgtgacg gtgttataat tcaccgcacc
    20101 gcccggtgag cgccagttac cgttgagcgt ttcgatcgct ttcaccgcga cgcgcgtctg
    20161 ttccggcgtc gcatccggat tggttaaccc tttcttcagg tattcttccc agcgttgctg
    20221 cgaggcggtg agataaaacg ccggacgcgc cagaatatcg cggatctgca tttgctcttt
    20281 gctaacttcc tgagcggtca gcagatggga ataggtggtg tagagcgtgg tcgaaccgtt
    20341 gatatgcgcc ttactggtaa agcgattacc gttgatttca gtctgcaccg gcagggattt
    20401 atgcacctga tattccgatt cgccggaggt cagcagatcc caggtggcgc gcactttgcc
    20461 aaaggtgact ttcaggccat cacgggtggc gctgattttg cgctgatagt cagggtattc
    20521 gccagcaatg gttttatcgg aaagcggttt cccttctttc gcttccagtt tttccagcag
    20581 ttcgccatcc cacaccagat ccagcggttt attgctgatg attttagttt ccagtagtga
    20641 cgtgcgcggc gtggcgaagc gcagagtcat ttcgacctgc acatcttttg ctgtcagttt
    20701 ttgcaccagc gcgccgggaa tactgtatgc ctccagcgtg aagttgactt tcttgccgtc
    20761 ttgccagaca gtcaggcggt cgaaattgct ggccataaag ttgatgtatt cttccgtcag
    20821 cagcgcaacg cccggaaagc cgcccatggt gttcgggccg tctggcaaca gatgaccatg
    20881 ccaggctccg agatcgaaaa acggattaaa acgctggtga tcgtcgtaat cgtaatcctt
    20941 catgtactgc ggcgcaccag tacggttaat cacgttttta taattgtcgg cgttagcggc
    21001 atgagcggaa aaacttatca gcagagcgca ggttactggc gttaaaatag ttttgatttt
    21061 catgagatta cagtccttat taccagtaga aatattgcat caggaagatt tggtcgctgg
    21121 gatcgttaga ggcatttttc atgtagaagc ggctatcaaa agccgtggcg aaacgtccgc
    21181 cgccatattc gtaccagata ccaaactgca tgaaagaggt ggtttcatcg tcagcgtggt
    21241 cagggcgatg tttggcgtag gtgtaagcgg tggagagata aacgcctttt aacgcttcat
    21301 acatggcgct caccatcatc ccgctttcac ttcccggtac gtccagcccg tcggcacgtc
    21361 cggtatggtg ccaggcgcga gcggcaaagt taggggtaaa caggcccgtg agatagagtt
    21421 gtcctgtgcc gtcggtaatt tccagaccat tcatccaggt gatgccttcg gcaaggtcat
    21481 actggatgta accgttcatc cattgccgga taggtgtatt tgtcgtcgta gcgatcgtac
    21541 ttgccaaaat gtagccaggc tttgctttcg tcatgatgtc cggcaggtgt ggcggtgacg
    21601 ctataacgaa agttgccgct taagttctgt accttcagcg cggtcataat gtcgcgggta
    21661 ttgggaatga cgtagcccaa atcgggtgtg aagtcgcccc accattgcag gtcatccagc
    21721 gaggaatcct gacgcagact gagcatgatt tcggtgccat cattggtacg ataaccgcca
    21781 tagaaacggt taatcccacc ttcgaaacca ccccagctat ggtcaggcac ctaggcatta
    21841 ccgtcgtggt cggcctgcac ggtccagcct tcgccgtaaa tcaggccgaa ccaggagccg
    21901 tacttcatct ccaggccacc ttcaatatag gtgccgtcgc tgtatttatg tttattctca
    21961 ccctctaaat ccatatatcc accaagccct aattcgccgt aaaaacgaaa tgaaggagaa
    22021 gatgtcgagg taattgcggg tgtttttgtg tcgtcatgtt ctgccggacg tgcttcttct
    22081 cctgccgcag aaaaggcgta gcaaaacggc agagcaagta atgctctgca tggtgcagtg
    22141 attaatttca ttgtgttaat ccatgagcta aagaaaaagt acagcctttt atcgttgtaa
    22201 ttataaaaat ataaaggcag aaactatttt ccctctcctc atccaggaag agggaaaatg
    22261 cctgctcatt ttttattagt ctgcttaatc aacattgctg gctggcgtcg cttcaatatg
    22321 gcgctcttca gccgttaatc cctttatata tttgctgtat ttccaccacg caaagccgag
    22381 gaagataaca ataccgccga cgttataaaa aatgatggtc agaatattcg cgccagtcgg
    22441 gaatgtcgaa gcgacaaacc ctacggcaaa tatcgcaatc agcattgaaa caacgataat
    22501 accggtgcgg cgggagccca tacggaaatc gcgtggcagg tgatctaatt tggcgcgtaa
    22561 attcaggtaa gccagcatga tgaataacgg cggaagcatg gacgctgcgg cggtcatatt
    22621 aataatggta ttcatcagat cctgcacggt attggaaccg agcatcggga taatcatcag
    22681 cgggatgacg atcagaaact ggatccatgc tgcgcgcgcc ggaacgccgt tttcgttaag
    22741 ttcgacggtt ttcttaccaa agatgccttc cgggatttcg gagaagaaaa ttttcaccgg
    22801 cgttgcggtc cacatcagca gggaaccgaa cattgcggta aaggacacca gaccgacaaa
    22861 gcgattcatc aacgcttccg gcagaccaaa atacgccgcc atgccgtgga atacctgcac
    22921 cgatccgccg gtaaatttca actctttgct gctgacgaag acgttaatca gcaccgagga
    22981 gacggaatac agtacgccga taaaaatccc ggcgaggatg atcactttaa cgaacgattt
    23041 cgaaccgcct ttgacgtcgt taacgtacac cgcgacggac tccgcgccac ctgcggccat
    23101 aaagatccag gtggtaacgc cgaggaacgc ccagttgaag ttcgggatca tcgcgtcaac
    23161 ggtgatcgga tcggcaggct gtacgccgcc aaccagtgct gtacctgcca gtaaaatgta
    23221 ggagagcgtt aacagcagca tcagcgttga agtgacggag gtaattggcc ccagcatttt
    23281 cgccccgttg gtggaaaccc aggtggagaa ggcgaacagc accatactga taacggtggt
    23341 ggcaaccggc gtcataatat attcgtagcc gaggaaggca tacgaagcat aagcaataac
    23401 gcgcggcaac agcgaggtga aaaagaacag atttacgaac cagtaagtgt aggcagtaat
    23461 aaatgcccaa cgtccgccca gcgaactttt tacccacgcg tagacaccgg cttctgagtt
    23521 tttatttaac gaaacaaatt ctgcgatgat cagacagaag ggaataaaat aaaaaatcgt
    23581 cgcgaggaaa aacatcggtg ccgaggccag tccaagctca atattattat tgataacgtt
    23641 gttaaagctg taaacggcgg caaaagtcag cgagagcaag ccgaatttgc cgattgtatt
    23701 acgtttggta tcagacataa cacatctccg aaaatgtgta gggtagcggt ggcggcaaac
    23761 tccttttgcc gccgtagttg ttatttgtta tggaaataac catcttcgat ggtgactttg
    23821 agaaccactt ttttgaccgc gttattgcag ataaaccgat acgcttcatg gatatcgcaa
    23881 ataacgattt gcccttcgtg gacctctacg gtttctccgc agccttttaa atattcacgg
    23941 tcagtttcat cacgataata ttccactacc tgtaatgtct ctttcggcgc atattcaatt
    24001 ttttgctgcc cttgcaggta gtaatgcact tcaaaatagc gtcgatgccc ggtaaatagc
    24061 gcatcggtcg cggagtcatt ttccacacgg taggtcaatg aatcaccgat ggagtgggcg
    24121 acgccaggct gaatgttgtc gatattttca atcgcttcaa cgcagcgttg ccacttcttg
    24181 ccagaggcgt aaatctggcg gaactgttct aagttatcga tgatcctcat tgcttattct
    24241 cgctgtgcaa attcgtggaa aagaaccctg cgccgaactc atacgacgcc aggctttgcg
    24301 cggtagcttc tccgccagaa accggcagca acgtaaagcc gtagctgaag tcacggaacc
    24361 agacgcgcca ggagtccagc acctcgctgc cccaggagtt ggagccgagg ccaagtagct
    24421 ggtggtcgag gttcagggtg atgtcatcac tgcgctgcag ctcgttacag tgctgggcag
    24481 cgtggatgtt ttcctgggta tagtgccagg cgctgaagtt aattgggcgc tgcggaacca
    24541 ccagcagacc gttgccgtgg cggttagtca gtgccgtcca gcggacatgc tgacggttgc
    24601 cgttgttctg cgggaaggga tagttctcga acatggcatc gacggtgctg cgccagatat
    24661 cgatgatgtt agcctgctgg ctgtcggcgt agttttcgcc cggtccacga ccgtaatacg
    24721 ccacctgatc gtattcgccg ttaatcccca tggtgaaacc gatgcacgga atgatgtgcg
    24781 gatagtcgcc gtaacgctcg ccggaaagcg ccacgttaac ctggccatcg gcagcgatgc
    24841 gccagatgta ggtacagcgc atcccgaagt caaacaccgg cggtgcaata actgtgcggc
    24901 tgatgatcaa cacttcacca tcgctctgtt ctacggcaaa gtcgcgcaga tgttcctgca
    24961 tgatctgcaa atgattcggc tgccacaacc cttcgtactc ctgcttgtgg ttgtcgatca
    25021 tcggcttgaa gaagttgatc tttggctcgc gggtcagcag cgactcgcca tttacctggc
    25081 aggatgtcgg tttgccactc atttttgaga aggtgatcgc gaagttgtag ccgcgaacgg
    25141 tgcagctcaa acgatcgtct tccagcgtca gcggacgcgc attatttggt gcgaaaggca
    25201 ctggctgcgc ggtgttttcc ttcagcggga actgataaga ggcgatagaa tgcccggctt
    25261 cgctgtagcg ggtgcgggaa tctttggtca ccgtaatgtt gaggaacgct tcgcgggcgt
    25321 ccagctgcgg cagcgtgatc tgcaaggggg cttcgctgtt cggcgcaacg tcgcgcagtt
    25381 taatctgctg cgtcgcgagc gtttcacctt cggcgcgcac ctctgcgtgc agggtgtagt
    25441 catcaagcgt ggtaaaccac agtttatttt cgactttcaa ctcgccgcga gtcagcgcgt
    25501 ggatttttac cggcgcgata acctgtttgt actctttcag gcccgatccc ggcgtctgat
    25561 cggaatagat caaaccatca agacagaagt tatagttgtt gggatagtcg ccgccgaatt
    25621 tataccagac attgccgtgg tcgtcctgtg cctggatccc gtggtcgcac cactcccaga
    25681 cataatgacc ctgaatgcaa tcgtgcttat agaagacgtt ctggtactcc gtcagcccgc
    25741 ccggtccgtt ccccatcgca tgagcatatt cacagatgat gcgcggcttc ggatgcgggt
    25801 attcaccaaa ctcattcatc agcggcacgc gggtgtacat ggtggaaata atatcgacca
    25861 cttcagcatc gcgatcttct tcgtaatgaa ccagtcgcgt gtcatccagc gctttcgccg
    25921 catggtacat cgcgcggatg ttacagccat agccggattc attgcccagc gaccagatga
    25981 tgatcgacgg atggtttttc tgtgcgtgga tatggcgaac aatgcgctcg acgtagactt
    26041 tttcccactg cggatcgtcg gtaatacggc tgatatcgcc gacattagca aagccgtgcg
    26101 attcgacgtc ggtttccgcc atcacaaaca ggccgtagat atcacacagt tcgtaaaaac
    26161 gcggatcgtt cgggtagtga gcggtacgca cggagttgat attgtgctgc ttcatcaact
    26221 ggagatcttt ctcgacgcga tccattccaa cggcgcggcc tttgcgatga tcgttgtcgt
    26281 gacggttgac gccgtgcagc atcacataac ggttattgat ccagaacaga ccgtcgcgca
    26341 ctttgatatc acggaagcca acgcgttgtg gcaccacttc cagaacgttg ccgttggcgt
    26401 ctttcagcgt catgaccaga tggtaaagat aaggggattc tgctgaccat tgctgcggct
    26461 gttcgacagt aaaagcaaag ctggcgctgg tcagtttttc aattgccaaa tgatcaatgg
    26521 cgctgctgtg caccacgcgt tcgccatcaa acagggtata ttccagcgtc gtgacgacag
    26581 gggaggcggc gagattttcc agcaccactt cgcaggaaag cgtggcatcg caataggctt
    26641 cgtcaaagtc ggtacgcaca gtgaaatcgt taatatgcgt taggtgtttt ccgaccagat
    26701 aaacatcgcg gaagatcccc gccgaccacc acatatcctg gtcttccacg taggtagagt
    26761 ccgcccactg catcacgcgc acacacaaca ggttgtcgcc ggttttaacc atcgcgctga
    26821 tgtcaaactc tgcggtcagg cgactgccct tgctgaaacc cacatactga ccgttaacgt
    26881 agacttcaaa ataggtttcg acgccgtcaa atttaatcag cgtctgttta ccctgccagc
    26941 cgtcgctgag ggtgaaaata cgttgatagg caccggttgg gttatcgctg gggacaaacg
    27001 gcacatcgat ggggaacgga aaaccttcgt cggtatattg cagtttgccg tgaccttcca
    27061 tttgccacat ggcggggacg gtaatatgcc cccagtcagc cattaactca gaggtgaagg
    27121 cttctggtac ttgcagcgga tggtcaaaaa agtggaaatt ccactgactg cttaagggca
    27181 gaaacaggct gctggtttcg cgggcaaagg tacgcgcttg cgcaacagaa tcatatgaaa
    27241 aaaagtacgc acgcggcgca agtcggtttt cgtgggtgag ctgaatgttt tcccagcgat
    27301 tcatagggcc tcccggtaga gagaaatcgg aaccattcat ggtgatggta gtttgtgtaa
    27361 ttgctggggg gttagtaaaa tttttactaa aaagcagcag gtaaatgcat ttactttaac
    27421 tggatcacga aaataaacgg cagcactgtc cggcaaaaat gattgccgga cagtgtgtaa
    27481 ggggatttaa cgggtcgtgc cgcgcagttt taatttgctg ggaacgaaga ctagcagcgg
    27541 cagcgcgcga ccatcgcggg ctttttcata caccaggtta acgccctgac ttcccatcat
    27601 ctcggaatgg atgcgcacgg tggagagcgg cggaaaggta aatcgcgcgg tggggatatc
    27661 gttaacgctg ataagcgaaa tatcttgtgg gatgttcagg ccacgttcat gaattgcccg
    27721 cagtacgccg atagcaatgg aatcggaagc aacaaacagt gccttcggat agtcttcccg
    27781 cgccagcatt tgttttgcca gttcataacc cgacgaactg gaaaaaccgc cgcgccagat
    27841 atcctcttcg cgtaccactt gtttcagtcg gccatattcc gcaaaggcga cctcacgaat
    27901 atccgccttg ccaggctcat cttcaccgcc aataaaacca atacgattaa cgccctggtt
    27961 gatatagaag tcgatgattt ctttactgat gcgtgccaga tcgatatcca ccgcatcgta
    28021 accgctgccg ggttcgtgaa agtcgataaa acagatattg tcggtcaacg cgctggcagc
    28081 ggcgcgcagg gcgggcgtgg gtttgccgac aattaaaata ccggtgacgt ttttaatgtc
    28141 tggtaatccg ttgtgttcat aacagttggt cagttcgatc gccagctttt cgcactgagt
    28201 ttcaatgccg tggcggatcg ccagatagta aggatcgttg atctccagct cctgctggta
    28261 gctgtagata gccagaatat ggtgttggtt gactgcgcct gtctgtagtt tacgggcgct
    28321 actggtcttg tactccagct tttcggcgat ctcgagaatg cgatgtttcg tctcttcttt
    28381 cacattcaat gtcggatcgt catttaagac cctggatact gtcgccaggg atacgccagc
    28441 ttcgattgcg atgtctttta atgttgccat gtttaccttt tttactttcc ctgatgtgcg
    28501 cccccattgt agaggaagtg cttatcgggt cacctgtttt cagtaaataa agcgcgtttc
    28561 tttgacgctg taacggtaac acagcggctt aggaatgtga tcacgcctgc attctccatt
    28621 tttagcctac aggattgaga aaaacttttc tatagtttag taaaatttta tccgaaggga
    28681 ggaagcaatg gaaaccgtgg cttacgctga ttttgcacgt ctggaaatgc gcgtcggaaa
    28741 gattgtggaa gtgaaacgcc atgaaaacgc cgacaagctg tacatcgtac aggttgatgt
    28801 ggggaaaaaa cactgcaaac cgtgaccagc ctggtgccgt actacagcga agaggagctg
    28861 atggggaaaa cggtggtggt cttatgcaat ctgcaaaaag cgaaaatgcg tggtgaaacg
    28921 tcggaatgca tgctgttgtg tgcggaaacc gatgacggca gtgaaagcgt gttgttaacg
    28981 ccggagcgga tgatgcctgc gggcgtacgc gtcgtataaa aagatcggat ggcgacgtcg
    29041 tatcgccatc cgatttgata ttacgcttct tcgacactta ctcgcatggc cgccagcgcc
    29101 ttacgcgccg ctttgatcac cagttcacac tgttcaatgg tcagtgtcag tggcggttca
    29161 atgcggatcg ttttggcgtt attgagcgtt ccggccacca gtacgcgctg gcggaacatc
    29221 tcgctggcaa agttatagcc gatttcgtta tcaacaaact caatcgccat caacatccct
    29281 ttaccacgcg cttcctgtac cagatcggga tattcccgcg ccagttgacg gaaaccgtcc
    29341 agcaacatat cgcctttttg ctcagcctga gccggtaagt tctgctccag caacacattg
    29401 atggtcgcca gcgccgccgc acaggccagc gggttgccgc caaaggtggt ggtatgcagg
    29461 aatgggttgt cgaacagaac tgaaaacacc tcttcagtgg cgatggtcgc gccaatcggc
    29521 atcacgccgc cgccgagggc ttttgccaga cacagaatgt ccggctgaac gttctcatgt
    29581 tcgcaggcaa acatcttgcc cgtgcgcccc atgcccgttt gtacttcatc gaggatcatc
    29641 agtgcgccga actcatcgca cagcttacgc acggcggtga gatagcccgg cggcggcaga
    29701 attacgccac cttcgccctg aatcggttcg aggatcaccg cagccacatc atcaccggtt
    29761 tttttgcact cattaagagc cgtgcgcatg gcttcgatat tgccaaacgg cacatgacgg
    29821 aaccctggca gcagtggcat aaacggtttg cggaaggtcg atttcgccgt ggccgacagc
    29881 gcgccaagtg atttaccgtg gaacgcgccg ctggtggcaa taaaagtaaa tttgccgcgc
    29941 ggcgactgat aagccttcgc cagcttcagc gccgcttcaa cggactcggt gccgctatta
    30001 cagaagaagc tgtatttcag tttaccgggc gttagcgcag caagggtttt cgccagcata
    30061 gcccgtaacg gatcgagtag ctcctggctg tgcagtggtt gtttcgcaag ttgattctgt
    30121 acggcggaaa ccacaactgg attacggtgc cccacgttga aaattccaaa acctcccagg
    30181 cagtcgataa actcctgtcc ctgggtgtcg acaagcgtat ttaaacctcc cgcttgccac
    30241 tctacggctc cgtaatcccc gccggcggta acagatttgc gatactctaa aaaccccgga
    30301 ttgacatgct ctttgaagta ttcaatcacc tctcggttaa gtgctttcat ctcctcatga
    30361 tccagcgttc gcttctcaat gagattcagg gcgtgggcgc tgcacgctaa agccgatgcg
    30421 ctcgaaggta acctgttcaa aatatgctcc ggaggctcgc gtatcacatg ataccgattt
    30481 aagtattgca gggattgcgc cactccggct gcgatcgcga aaatcgggat aaacacaaat
    30541 gcggttatca tttgtgcaat atttaatgct atccgttaac atttgttgtt attttgcacg
    30601 gtttttgtcg ggtatggatt tgatgattgc tgcgcaaatg cacgttttca gggcaataat
    30661 tgcctgattt ttgggcaaga taattaacgt tttcgtatag taacagagtg aaagtatttt
    30721 aaatcaaaga attagatttg tgctttaatc gcaaattgcg atctaaatca aattaatcgg
    30781 ttaaagataa ccgcagcggg gccgacataa actctgacaa gaagttaaca accatataac
    30841 ctgcacagga cgcgaacatg tcttctcatc cgtatgtcac ccagcaaaat accccgctgg
    30901 cggacgatac cactctgatg tccactaccg atctgcaaag ctatatcact catgctaatg
    30961 acacttttgt gcaggtgagc ggctttacct tgcaagagtt acaagggcag ccgcacaaca
    31021 tggtgcgtca cccggatatg ccaaaagcgg cgtttgcgga tatgtggttc accctgaaaa
    31081 aaggggagcc ctggagcggc atcgtgaaaa atcgccgcaa aaatggcgac cattattggg
    31141 tgcgggccaa tgcggtaccg atggtgcgcg agggaaaaat cagtggctat atgtcgattc
    31201 gtacccgggc gacggatgaa gagattgcgg cggtggagcc gctgtacaaa gcgttgaact
    31261 ccggacgtac cagtaagcgt attcataaag gcctggtggt gcgtaaaggc tggctgggta
    31321 aactgccttc attaccgctt cgctggcgga cgcgtggagt gatgaccctg atgtttatct
    31381 tgctggcggc catgctttgg tttgttgctg ccccggtggt gacgtatttc ctctgtgtgt
    31441 tagtggtatt gttggcaagc gcttgttttg aatggcagat tgtgcgcccg atagaaaatg
    31501 tcgcccgtca ggcactgaag gtggcgaccg gagagcgtaa tagtgttgag cacctgaatc
    31561 gcagcgatga gctggggctg acattacgcg cggtagggca gcttggcctg atgtgccgtt
    31621 ggctaattaa cgatgtctca agccaggtgt ccagtgtcag aaatggcagt gagacgctgg
    31681 cgaaaggcac cgatgaactg aacgaacata cccagcagac agttgataac gttcagcaaa
    31741 cggtggcgac catgaaccaa atggcggcgt cggtgaaaca gaactctgcc acggcgtcgg
    31801 ctgccgataa actttctatc accgccagta atgcggcagt gcagggtggg gaggcgatga
    31861 ccacggtgat caagacaatg gacgatatcg ccgacagtac ctagcgcatt ggcaccatta
    31921 cttcgctgat taacgatatt gcgtttcaga ccaatattct ggccctgaat gcggcggtgg
    31981 aagcggcgcg tgccggcgaa cagggcaaag gttttgcagt ggtggcaggg gaagtgcgtc
    32041 atttagccag ccgcagcgct aatgctgcca acgatattcg caagctgatt gatgccagtg
    32101 ctgataaggt gcaatccggt tcgcagcagg tacacgccgc cggacggacg atggaagata
    32161 ttgtggcaca ggtgaaaaac gtcacccagt tgatcgccca gattagccat tcaacgctgg
    32221 aacaggccga tgggctttcc agcctgaccc gtgcagtgga tgagcttaac ctgatcaccc
    32281 agaaaaatgc cgagctggtg gaagagagtg cgcaggtgtc ggcgatggtg aaacaccgcg
    32341 ccagccgact ggaagacgcg gtgacggtgc tgcattaatc ggtatgccgg atccggcggt
    32401 tggagcacaa tgcctgatgc gatgctggcg catcttatca ggcctgcggg gtgtgtagcg
    32461 ggtgtaggcc ggataaggcg cgtcaagcgt cgcatccggc aattgcaccg cgccactggc
    32521 ggatgcggcg attaatccag ttgcgtaata tcaaaagcgg cgcggtcgat caccgcaatg
    32581 atctttttta tttgtgcatc actgatatcg ctctggttga cgcgtaaatc cagcaccgct
    32641 ttaaaattat ccagcgcccg cttcatttgc gggttctggc gcagcgccgc gccaacgcaa
    32701 cgcgctttga tgcgttcttc aatcatctcc acttgttcgc ggttttcttc cagccactgc
    32761 gcaccttgtt cggtcagcgc aatctgcttc ttaccacctt cctcttcgcg gatggtaatc
    32821 agcgactgct cctgcagaaa atccagcgtc gggtagatga cgcccgggct tggggtgtaa
    32881 ttcccctggg ttagattctc aatcgcttta atcaattcgt aaccgtggct gtcatcgcgc
    32941 gagagaatat ccagaatcac cagacgcaat tcaccgtgac caaagaaacg ttgccgacga
    33001 ccgccgccgc gaccgtggcg accaccgcaa cattgaccgt gttcatgctg gtgaccgtgt
    33061 ccgcagtgct cgtgttctga cttctcacct ttgcagcagc cctcatgccg tggctggcct
    33121 tcatgtttac aacacccttc gtgatgatgg ctcatatcag cctccttttg cttgattaga
    33181 tatatctaat ttatatctga tgtgagcatt actgcaagta caaaaatgat ttttaaacgt
    33241 ggaagcacct cacatctagg ccggataaga cgttcacgcc gcatccggca gcattgctat
    33301 ggcactgaat gccttcacat acttcagaca tatcagttct acaaatcgct tgcatttatc
    33361 atgattaaca atcattatca tttgcgaatt ttatttagat atatctgatt accatcacga
    33421 aggcgataac aatgaataac accccccgct acccgcagcg cgttcgcaat gatctgcgct
    33481 tccgtgaact gactgtgcta cgcgttgagc gcatcagcgc cggttttcag cgcattgtcc
    33541 ttggcggcga ggcgttggac ggttttacat cgcgcggctt tgacgatcac agcaaactct
    33601 tctttcctca acctgacgct cactttgtgc cgccaacggt aacggaagag agcatcgtct
    33661 ggccggaagg accacgccca ccgtcgcgtg actatacgcc actgtatgac gaactacgcc
    33721 atgaactggc gatcgatttc tttattcacg acggtggggt cgccagcggc tgggcgataa
    33781 acttacggtg gcaggtccgc gcggttcgct ggtggtgccg gaagattacg cgtatcagct
    33841 gtatgtctgc gatgaatccg gaatgcccgc actgcgccgc cgcctggaaa cgttgagcaa
    33901 acttgccgtt aaaccgcaag ttagtgcgct ggttagcgtg cgggataacg cctgtcagga
    33961 ttatctcgcg caccttgatg gttttaatat cgaatggctg gcacatgatg agcaggcggt
    34021 agatgcgcgt ctggcgcaga tgcaaatccc tgccgatgat tacttcatct ggataaccgg
    34081 cgaaggtaaa gtcgttaaga atttaagccg ccgctttgaa gcggaacagt atgacccaca
    34141 gcgggtacgt gcagcggctt actggcacgc aaagtaactg atatataaaa agaaaaaggc
    34201 tgacgatttc tcgtcagcct ttgctatatc tggtggcccc tgctggactt gaaccagcga
    34261 ccaagcgatt atgagtcgcc tgctctaacc actgagctaa ggggccatgg tagcggatta
    34321 taaagtaact ccgtgtcgca atccagccat taccgcgcgc ctgctgtttt tataaacaat
    34381 gtattttcaa tccgttatac ttttcctggt gatgttagaa aggagtaaat atggttgagg
    34441 atattttggc tccagggtta cgggtcgtgt tttgcggtat caaccctggg ctttcatccg
    34501 ccgggactgg ttttcccttt gctcatccgg caaatcgctt ctggaaggtg atatatcagg
    34561 ccgggtttac cgaccgtcag ttgaagccgc aggaggcaca gcatctgctg gattatcgtt
    34621 gtggcatcac caaactggta gaccgtccaa cggtgcaagc caatgaagtt tcaaagcagg
    34681 agctgcacgc aggtgggcgt aagctgattg aaaaaattga agattatcag ccgcaggcgt
    34741 tggcgattct gggcaaacaa gcatatgaac agggattcag ctagcgcggt gcacagtggg
    34801 ggaaacaaac gctcaccatt ggttcgacgc agatttgggt gctgccaaat cccagcggtt
    34861 taagtcgcgt ttcactggag aaactggttg aagcgtatcg cgagctggac caggcgctgg
    34921 tagtgcgtgg gcgataaaa
//
LOCUS       DAJTQY010000033        34445 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.033, whole genome shotgun sequence.
ACCESSION   DAJTQY010000033 DAJTQY010000000
VERSION     DAJTQY010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34445
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.033"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(<1..332)
                     /locus_tag="OO349_001705"
     CDS             complement(<1..332)
                     /locus_tag="OO349_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5866814.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLTLTLLPLFAVTADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADINSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQ"
     gene            702..1652
                     /gene="corA"
                     /locus_tag="OO349_001706"
     CDS             702..1652
                     /gene="corA"
                     /locus_tag="OO349_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HCR5866815.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(1693..2073)
                     /locus_tag="OO349_001707"
     CDS             complement(1693..2073)
                     /locus_tag="OO349_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HCR5866816.1"
                     /translation="MSKEYMNDDSLSEKWKYRFNFYDQHGFPGFWGATPEYKAAFKAL
                     KVRQRLTIQMNFIAFFCSWIYLFVLGLWKKAIIVLLLGILSLFVGALIGVNILGIAVA
                     AYVAVNTNKWFYEKEVKGLNTWSL"
     gene            complement(2087..2503)
                     /locus_tag="OO349_001708"
     CDS             complement(2087..2503)
                     /locus_tag="OO349_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5866817.1"
                     /translation="MLTGDSHKDVKFMLRMFIPTSNGKISRHRYIFLFILINFIFAFL
                     IIFFNDGEAGFLVIVSTIALHYLVINMNCQRLRDSGFIYIKIYVFGTLAVYIISIITM
                     IAEDFACSGNGSMIFLICYFSTFSMLMLAPTDSSKQ"
     gene            complement(2562..3452)
                     /gene="rarD"
                     /locus_tag="OO349_001709"
     CDS             complement(2562..3452)
                     /gene="rarD"
                     /locus_tag="OO349_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HCR5866818.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFTFI
                     WVALAIFVMDAIYTQRRTSK"
     gene            complement(3504..3971)
                     /locus_tag="OO349_001710"
     CDS             complement(3504..3971)
                     /locus_tag="OO349_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HCR5866819.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            4136..5005
                     /gene="pldA"
                     /locus_tag="OO349_001711"
     CDS             4136..5005
                     /gene="pldA"
                     /locus_tag="OO349_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HCR5866820.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            5138..6967
                     /gene="recQ"
                     /locus_tag="OO349_001712"
     CDS             5138..6967
                     /gene="recQ"
                     /locus_tag="OO349_001712"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HCR5866821.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSRGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            7031..7651
                     /gene="rhtC"
                     /locus_tag="OO349_001713"
     CDS             7031..7651
                     /gene="rhtC"
                     /locus_tag="OO349_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HCR5866822.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTERWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(7713..8333)
                     /gene="rhtB"
                     /locus_tag="OO349_001714"
     CDS             complement(7713..8333)
                     /gene="rhtB"
                     /locus_tag="OO349_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HCR5866823.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            8444..9466
                     /gene="pldB"
                     /locus_tag="OO349_001715"
     CDS             8444..9466
                     /gene="pldB"
                     /locus_tag="OO349_001715"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HCR5866824.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATQTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            9474..10274
                     /gene="yigL"
                     /locus_tag="OO349_001716"
     CDS             9474..10274
                     /gene="yigL"
                     /locus_tag="OO349_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HCR5866825.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            10350..11249
                     /gene="bioP"
                     /locus_tag="OO349_001717"
     CDS             10350..11249
                     /gene="bioP"
                     /locus_tag="OO349_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HCR5866826.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRSNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQYNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(11137..12090)
                     /gene="metR"
                     /locus_tag="OO349_001718"
     CDS             complement(11137..12090)
                     /gene="metR"
                     /locus_tag="OO349_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HCR5866827.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            12326..14587
                     /gene="metE"
                     /locus_tag="OO349_001719"
     CDS             12326..14587
                     /gene="metE"
                     /locus_tag="OO349_001719"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HCR5866828.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALSHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDNVVEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDISRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(14626..15441)
                     /locus_tag="OO349_001720"
     CDS             complement(14626..15441)
                     /locus_tag="OO349_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCR5866829.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            15703..16464
                     /gene="udp"
                     /locus_tag="OO349_001721"
     CDS             15703..16464
                     /gene="udp"
                     /locus_tag="OO349_001721"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HCR5866830.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTVSSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL"
     gene            16605..18032
                     /gene="rmuC"
                     /locus_tag="OO349_001722"
     CDS             16605..18032
                     /gene="rmuC"
                     /locus_tag="OO349_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HCR5866831.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            18127..18882
                     /gene="ubiE"
                     /locus_tag="OO349_001723"
     CDS             18127..18882
                     /gene="ubiE"
                     /locus_tag="OO349_001723"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HCR5866832.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            18896..19501
                     /gene="ubiJ"
                     /locus_tag="OO349_001724"
     CDS             18896..19501
                     /gene="ubiJ"
                     /locus_tag="OO349_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HCR5866833.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            19498..21138
                     /gene="ubiB"
                     /locus_tag="OO349_001725"
     CDS             19498..21138
                     /gene="ubiB"
                     /locus_tag="OO349_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HCR5866834.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            21217..21486
                     /gene="tatA"
                     /locus_tag="OO349_001726"
     CDS             21217..21486
                     /gene="tatA"
                     /locus_tag="OO349_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HCR5866835.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            21490..22005
                     /gene="tatB"
                     /locus_tag="OO349_001727"
     CDS             21490..22005
                     /gene="tatB"
                     /locus_tag="OO349_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HCR5866836.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            22008..22784
                     /gene="tatC"
                     /locus_tag="OO349_001728"
     CDS             22008..22784
                     /gene="tatC"
                     /locus_tag="OO349_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HCR5866837.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSSEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            22826..23608
                     /gene="tatD"
                     /locus_tag="OO349_001729"
     CDS             22826..23608
                     /gene="tatD"
                     /locus_tag="OO349_001729"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HCR5866838.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(23605..24093)
                     /gene="rfaH"
                     /locus_tag="OO349_001730"
     CDS             complement(23605..24093)
                     /gene="rfaH"
                     /locus_tag="OO349_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HCR5866839.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTISATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPAPPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            24260..25753
                     /gene="ubiD"
                     /locus_tag="OO349_001731"
     CDS             24260..25753
                     /gene="ubiD"
                     /locus_tag="OO349_001731"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HCR5866840.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGEHFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IDPGEMAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            25799..26500
                     /gene="fre"
                     /locus_tag="OO349_001732"
     CDS             25799..26500
                     /gene="fre"
                     /locus_tag="OO349_001732"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HCR5866841.1"
                     /translation="MTTLSCKVTSVAAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDLPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHL
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(26692..27855)
                     /gene="fadA"
                     /locus_tag="OO349_001733"
     CDS             complement(26692..27855)
                     /gene="fadA"
                     /locus_tag="OO349_001733"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HCR5866842.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHIGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(27865..30054)
                     /gene="fadB"
                     /locus_tag="OO349_001734"
     CDS             complement(27865..30054)
                     /gene="fadB"
                     /locus_tag="OO349_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HCR5866843.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLIEGAMAILRQAINGDLDWKAKRQPK
                     QEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            30244..31575
                     /gene="pepQ"
                     /locus_tag="OO349_001735"
     CDS             30244..31575
                     /gene="pepQ"
                     /locus_tag="OO349_001735"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HCR5866844.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRYTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            31575..32189
                     /locus_tag="OO349_001736"
     CDS             31575..32189
                     /locus_tag="OO349_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HCR5866845.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            32228..33679
                     /gene="trkH"
                     /locus_tag="OO349_001737"
     CDS             32228..33679
                     /gene="trkH"
                     /locus_tag="OO349_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HCR5866846.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            33691..34236
                     /gene="hemG"
                     /locus_tag="OO349_001738"
     CDS             33691..34236
                     /gene="hemG"
                     /locus_tag="OO349_001738"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HCR5866847.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMLKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         8104 a         8702 c         9305 g         8334 t
ORIGIN      
        1 ttctgctgac cgttttcgat gtacaaaccg agcggcgcat agctttcatc atagatgccg
       61 ccgttcatcg ccatctgcac ctgaccctga ctattaatat ccgccagcag agcatgtaac
      121 gttccccacg cttcgccatt ggctttttgc cagtacattt tcacccgctc tgtttgagga
      181 ttaacggtat acgcctgtac ggtcagcgtc ggatctgaga gtgcgcaatc atcagcggta
      241 acagcaaaca agggaagtaa ggtgagggtg agaaaaatcc gtttgagatt caaggtgatc
      301 attcctttac caatgagtag ccgatgcgcc attataggtc ctagatgtgg gattttttta
      361 tcctgttagc gaccttgacg agtaccaaaa agcgcgaagt tcaactattg ttctgtggtg
      421 ttctgttgcg tgttgacggc aaaattttgc tggcgtaaca tgcgcgcacg atcactctaa
      481 gaggacattc gccttggaca cacccagtag atactggctc actatcctgt catccaggat
      541 caactcctaa ggctatccct ttttgctgat agccttagcg gttgtcagcg acctcaattt
      601 ttcccgtcgc gctgagtcag gctgtttaat ggtctgaaac ccaatttgtt tctgtgtgcc
      661 caccgaactg tccgatattt taagcattgg gagtcccggt catgctgagc gcatttcaac
      721 tggaaaataa ccgactgacc cggctggaag tcgaagagtc acaacccctt gtaaatgcag
      781 tatggattga tcttgtcgaa ccggacgacg acgagcgact gcgcgtacaa tctgaacttg
      841 gccagagcct ggcaacccgc ccggaactgg aagacatcga agcatcggca cgtttctttg
      901 aagacgacga cggcctgcat attcactcct tcttcttctt tgaagatgcg gaagatcacg
      961 ccggtaactc cactgtggca tttaccatcc gtgatggtcg tctgtttact ctgcgtgagc
     1021 gtgaactgcc cgcttttcgt ctgtatcgta tgcgtgcccg tagccagtcg atggtagacg
     1081 gtaacgccta cgagttgctg ctggatctgt tcgaaaccaa aatcgaacag ttggcagatg
     1141 aaattgaaaa tatctatagc gacctggagc agttgagccg ggtgattatg gaagggcatc
     1201 agggcgatga gtacgacgag gcgctctcca cgctggcgga actggaagat atcggctgga
     1261 aagttcgcct gtgtctgatg gatacccagc gcgctctcaa cttcctggtg cgtaaagcgc
     1321 gtttaccggg tgggcaactg gagcaggcgc gtgaaatcct gcgcgatatc gaatccctgc
     1381 tgccgcataa cgaatccctg ttccagaagg tgaacttcct gatgcaggcg gcaatgggtt
     1441 ttatcaacat cgagcagaac cgcatcatca aaattttctc ggtggtatcc gtcgtgttcc
     1501 tgccgccaac gctggttgct tccagctacg ggatgaactt tgagtttatg ccagaactga
     1561 agtggagttt cggctaccct ggcgcgatta tctttatgat cctcgcgggc ctggcaccgt
     1621 atctgtactt taagcggaag aactggttgt aaaaagaagg cggtggctta gcctggctaa
     1681 gccacctgtt attcaaaggc tccaggtatt taaccctttt acctctttct cataaaacca
     1741 tttgttcgtg ttaacagcaa cataggctgc tacggcaatt cccagaatgt taacgccaat
     1801 taatgcacca acaaatagag atagaatgcc aagtaataga actataattg cttttttcca
     1861 taagcccaga acaaataaat agatccagga acagaagaaa gcaatgaaat tcatttgaat
     1921 agttaatcgt tgtcttactt ttaatgcttt aaatgctgct ttatattctg gtgttgctcc
     1981 ccagaatcca ggaaagccat gttgatcata aaaattaaat cggtatttcc atttttcact
     2041 taatgaatca tcgttcatat attccttact cataaatact ccataactat tgttttgatg
     2101 aatcagtagg tgcaagcatt agcatactga aagtggaaaa ataacaaatc aaaaaaatca
     2161 tcgaaccatt gcctgaacag gcaaaatctt cggctatcat tgtgatgata gagatgatat
     2221 atactgctaa tgtaccaaaa acataaattt ttatatatat gaaaccacta tcacggagtc
     2281 gttggcaatt catgttgatg acgagataat ggagtgcgat ggtagagact ataacaagaa
     2341 aacctgcttc cccatcgtta aaaaagataa taagaaaggc aaaaatgaaa tttattaaaa
     2401 taaataaaaa tatatatcgg tgtctggaaa tcttaccgtt agatgttggg ataaacatac
     2461 gtaacataaa ttttacatcc ttgtgtgagt ctcctgtcag catggcaata tgcccactct
     2521 catacaagag tcggcatatt ttttcagaat atatttattt tttatttgga cgttctacgc
     2581 tgcgtataaa tcgcgtccat cacaaaaatt gccagcgcca cccaaataaa ggtgaaagtc
     2641 accatcttat cggcacccgg tttttcacca taaaacgtca cagccagcag gaacatcagc
     2701 gtcgggccaa tgtactgaaa aaagcctaac gttgagagac gcaggcgcgt ggcagcggca
     2761 gtaaaacaca acagcggtac ggtagtgaca ataccggcgg cgatcagcag taaattcagc
     2821 gacatcgggt tttgccccat atggctggtt gaactgtcgg caatagcaaa caggtaaatc
     2881 gccgccacgg gcagcagcca catggtttca attaacatgc cggtttgtgc ttctaccgca
     2941 atcttcttgc gcaccaggcc gtagaaggca aaactaaatg ccagccccag cgcgataata
     3001 ggtagcgaac caaaagtcca cagctggacc aacacgccac atatcgccag aatcaccgcc
     3061 agccattgca tccggcggaa tcgctcgccg aggaaaatca tccccagcac aatgttcacc
     3121 agcgggttaa taaagtaacc aaggctcgct tccagcatat ggtgattgtt caccgcccag
     3181 ataaacagta gccagttgcc accaatcagt acggcagaga ctgccagcat aaaaattttc
     3241 tgtggcgtct gaatcagcgt ttttaaatag gaccactggc ggcaaatgct catcagcacc
     3301 accataaaga aaaacgacca gatcacgcga tgcgtcagga tctcatcggc gggcacgtag
     3361 taaatcagct tgaagtatgc tggcgcaata ccccaaataa aataagcggc aagagcgagt
     3421 aatacgccct gccgcgtttg ttttgcatcc atcgggaata ctcattttta attggtaaca
     3481 gcagtttacc tgcttttatg tcttcaaccc accatatagg tggcggtggc actggcaata
     3541 taaagctgtt cttcattgtg taattcaacg cgggcgacgg cgactttatt gcctgcacgc
     3601 aacaggctac tggtagcagt aaaacgctcg cccctgcctg ggcgcagata atcaacgcga
     3661 agatcaatgg tccccatccg cgatagccgc tggcgtagtt catcttcact gatggtttcg
     3721 tggcgggtta aggtacttcc cacgcacacc aggccggcgg cgacatccag cgccgacgca
     3781 atgactccgc cgtgcaaaat gctttgcgcc cagttaccca ccatcatcgg ctgattttta
     3841 aaggccagct gtgcgaactc tttttcgtaa cgctccagtt ccatccccaa tgcgcggtta
     3901 aatggcatgt gataaacaaa catctcaccc actaatttca gggcttgttc agcggtcagt
     3961 acggcagaca tatcatcctt acacttcatt ggttaatgaa atgttgattt tatgcttctt
     4021 tgttgttggt ttctacttta ggaagggata actaacggca atggagttaa gtatgtaaaa
     4081 tagcccgcag aaaaacattc accttatcaa taattcgtta cggagaacac gaccgatgcg
     4141 gactctgcag ggctggttgt tgccggtgtt tatgttgcct atggcagtat atgcacaaga
     4201 ggcaacggtg aaagaggtgc atgacgcgcc agcggtgcgt ggcagtatta tcgccaatat
     4261 gctgcaggag catgacaatc cgttcacgct ctatccttat gacaccaact acctcatcta
     4321 cacccaaacc agcgatctga ataaagaagc gattgccagt tacgactggg cggaaaatgc
     4381 gcgtaaggat gaagtaaagt ttcagttgag cctggcattt ccgctgtggc gtgggatttt
     4441 aggcccaaac tcggtgttgg gtgcgtctta tacgcaaaaa tcctggtggc aactgtccaa
     4501 tagcgaagag tcttcaccgt ttcgtgaaac caactacgaa ccgcaattgt tcctcggttt
     4561 tgccaccgat taccgttttg caggttggac gctgcgcgat gtggagatgg ggtataacca
     4621 cgactctaac gggcgttcag acccgacctc ccgcagctgg aaccgccttt atactcgcct
     4681 gatggcagaa aacggtaact ggctggtaga agtgaagccg tggtatgtgg tgggtaatac
     4741 tgacgataac ccggatatca ccaaatatat gggttactac cagcttaaaa tcggctatca
     4801 cctcggtgat gcggtgctca gtgcgaaagg acagtacaac tggaacaccg gctacggcgg
     4861 cgcggagtta ggcttaagtt acccgatcac caaacatgtg cgcctttata ctcaggttta
     4921 cagcggctat ggcgaatcgc tcatcgacta taacttcaac cagacccgtg tcggtgtagg
     4981 ggttatgcta aacgacttgt tttgatgaac ggttgagtgg ttggcaaatc tggaatccag
     5041 catccaggat taccctctca gagactaaaa gcattgcagt ttctcgcgca ggcgctgaaa
     5101 atagcgcctg tttttatttc aggcaatcgg ggtgaatgtg gcgcaggcgg aagtgttgaa
     5161 tctggagtcc ggagctaaac aggttttaca agaaaccttt ggctaccaac agtttcgccc
     5221 cggccaggaa gaaattatcg acactgtgct ttccggtcgc gattgcctgg ttgttatgcc
     5281 cactggtggc ggaaaatccc tttgctatca aattcctgcc ttattgctaa acggccttac
     5341 cgtggttgtt tcaccgctga tttcgttgat gaaagatcag gtggatcaac tacaggccaa
     5401 cggcgtggcg gcggcgtgcc ttaactcgac gcaaacccgc gaacagcaac ttgaagtgat
     5461 gacaggctgc cgcaccgggc aaattcgctt actgtatatc gccccggaac gcctgatgct
     5521 ggataacttt cttgagcatc tggcgcactg gaatccggtg ttattagccg tcgatgaagc
     5581 gcactgtatc tcccaatggg gccacgattt ccgcccggaa tatgccgcgc tcggtcagtt
     5641 gcgccagcgg ttcccgacgc tgccgtttat ggcgctgacc gccacagccg acgacaccac
     5701 gcgccaggat atcgtgcgcc tgctggggct gaacgatccg ctgattcaaa tcagcagttt
     5761 tgaccgtccg aatattcgct acatgctgat ggagaaattc aaaccgctcg atcagttgat
     5821 gcgctacgtg caggaacagc gcggtaagtc tggcattatc tactgcaaca gccgggcgaa
     5881 agtagaagac accgctgcgc gcctgcaaag caggggtatt agcgcggcgg cctatcatgc
     5941 cgggctggaa aataatgtcc gcgccgacgt gcaggagaaa ttccagcgcg atgatctgca
     6001 aattgtggtg gcgacggtgg cgttcggcat gggcatcaat aaaccaaacg ttcgcttcgt
     6061 ggtccacttt gatattccgc gcaatatcga atcctattat caggaaaccg gtcgcgccgg
     6121 gcgtgatggc ctgcctgcgg aagcgatgct gttttacgat ccggctgata tggcgtggct
     6181 gcgccgttgt ctggaagaga agccgcaggg gcagttgcag gatatcgagc gccacaaact
     6241 caatgcgatg ggcgcgtttg ccgaagcgca aacttgccgt cgtctggtat tgctgaacta
     6301 ttttggcgaa gggcgtcagg agccgtgcgg gaactgcgat atctgcctcg atccgccgaa
     6361 acagtacgac ggttcaaccg atgctcagat tgccctttcc accattggtc gtgtgaatca
     6421 gcggtttggg atgggttatg tggtggaagt gattcgtggt gctaataacc agcgtatccg
     6481 cgactatggt catgacaaac tgaaagtcta tggtatgggc cgtgataaaa gccatgaaca
     6541 ttgggtgagc gtgatccgcc agctgattca tctcggcctg gtgacgcaaa atattgccca
     6601 gcattctgcc ctacaactga cagaggccgc gcgcccggtg ctgcgcggcg aatcctcttt
     6661 gcaacttgcc gtgccgcgta tcgtggcgct caaaccgaaa gcgatgcaga aatcgttcgg
     6721 cggcaactat gatcgcaaac tgttcgccaa attacgcaaa ctgcgtaaat cgattgccga
     6781 tgaaagtaat gtcccgccgt acgtggtgtt taacgacgca accttgattg agatggctga
     6841 acagatgccg atcaccgcca gcgaaatgct cagcgttaac ggcgttggga tgcgcaagct
     6901 ggaacgcttt ggtaaaccgt ttatggcgct gattcgtgcg catgtcgatg gcgacgacga
     6961 agagtagtca gcagcataaa aaagtgccag tatgaagact ccgtaaacgt ttcccccgcg
     7021 agtcaaatgt atgttgatgt tatttctcac cgtcgccatg gtgcacattg tggcgcttat
     7081 gagccccggt cccgatttct tttttgtctc tcagaccgct gtcagtcgtt cccgtaaaga
     7141 agcgatgatg ggcgtgctgg gcattacctg cggcgtaatg gtttgggctg ggattgcgtt
     7201 gcttggcctg catttgatta tcgaaaaaat ggcctggctg catacgctga ttatggtagg
     7261 cggtggcctg tatctctgct ggatgggtta ccagatgcta cgcggtgcac tgaaaaaaga
     7321 ggctgtatct gcgcctgcac cacaggttga gctggcgaaa agcgggcgca gtttcctgaa
     7381 aggcttactg accaatctcg ccaatccgaa agcgattatc tattttggtt cggtattctc
     7441 attgtttgtc ggcgataacg ttggcactac cgagcgctgg ggcattttcg cgttgattat
     7501 tgtcgaaaca ctggcgtggt ttaccgtcgt tgccagtctg tttgccctgc cgcaaatgcg
     7561 ccgtggttat caacgtctgg cgaagtggat tgatggtttt gccggggcgt tatttgccgg
     7621 atttggcatt catttgatta tttcgcggtg atgccagacg cgtcttcaga gtaagtcgga
     7681 taaggcgttt acgccgcatc cgacattatt tttcacgcat gcctcgccga tgctaacagc
     7741 gctcccacca gcataaacaa cgagccgaaa atcttattca gcgccttcat ctgctttggt
     7801 cctttaatcc atagagcaat cctttgagca agggtggcgt aaccgatcat cacaataata
     7861 tcgaccacaa tagtggtgac gccgagcacg atatactgca tcagttgcgg ctgttgcggc
     7921 atgatgaatt gcggaaatag cgccgccaga aacacaatac ttttgggatt ggtgagattc
     7981 acaaaaactg cgcgctggaa caaatgtcga cgcgattgag tagaggccag cgatttaaga
     8041 tcaattgcgc cagcggcgcg ccactgctgg attcccagcc aaatcaagta agccgcgcct
     8101 gcccacttta acacttcaaa cgcaatcact gagcgggaaa atagcgtccc caaccccacg
     8161 ccaaccagca caatatgaat cgccagtccg gtctgaagcc cagcaataga cgccaccgcg
     8221 ccgcgataac cgtggttgag cgaggtggtc atagtgttga ttgcaccaga gcctggcgac
     8281 aggcttaaaa tgatcgatgt cagcaggtag gcaaaccacc attctaaggt catgatgaac
     8341 tcccggtgtg tctatttttg tgccacaata cgctactgtc gcagcgttgt gtcaggcacg
     8401 cttaaaaaaa cgattttacg tggtttaaga ggcagattac ccgatgtttc agcagcaaaa
     8461 agactgggaa acaagagaaa acgcgtttgc tgcttttacc atgggaccgc tgactgattt
     8521 ctggcgtcag cgtgatgaag cagagtttac tggtgtggat gacattccgg tgcgctttgt
     8581 ccgttttcgc gcacagcacc atgaccgggt ggtagtcatc tgcccggggc gtattgagag
     8641 ctacgtaaaa tatgcggaac tggcctacga cctgttccat ttggggtttg atgtcttaat
     8701 catcgaccat cgcgggcagg gacgttccgg tcgcctgtta gccgatccgc atctcgggca
     8761 tgttaatcgc tttaatgatt atgttgatga tctggcggca ttctggcagc aggaggttca
     8821 gcccggtccg tggcgtaaac gctatatact ggcacattcg atgggcggtg cgatctccac
     8881 attatttctg caacgccatc caggtgtatg tgacgccatt gcgctaactg cgccaatgtt
     8941 tgggatcgtg attcgtatgc cgtcatttat ggcacggcag atcctcaact gggccgaagc
     9001 gcatccacgt ttccgtgatg gctatgcaat aggcaccggg cgctggcgcg cgttgccgtt
     9061 tgctatcaac gtactgaccc acagcagaca gcgatatcga cgtaacttac gcttctatgc
     9121 tgatgaccca acgattcgcg tcggtgggcc gacctaccat tgggtacgcg aaagtattct
     9181 ggctggcgaa caggtgttag ccggtgcggg tgatgacgcc acgcaaacgc ttctcttaca
     9241 agctgaagag gaacgcgtgg tggataaccg catgcacgac cgtttttgtg aactccgcac
     9301 cgccgcgggc catcctgtcg aaggaggacg gccgttggta ataaaaggtg cttaccatga
     9361 gatccttttt gaaaaggacg caatgcgctc agtcgcgctc cacgccatcg ttgatttttt
     9421 caacaggcat aactcaccca gcggaaaccg ctctacagag gtttaaattt cttatgtacc
     9481 aggttgttgc gtctgattta gatggcacgt tactttctcc cgaccatacg ttatcccctt
     9541 acgccaaaga aactctgaag ctgctcaccg cgcgcggcat caactttgtg tttgcgaccg
     9601 gtcgtcacca cgttgatgtg gggcaaattc gcgataatct ggagattaag tcttacatga
     9661 ttacctccaa tggtgcgcgc gttcacgatc tggatggcaa tctgattttt gctcataacc
     9721 tggatcgcga cattgccagc gatctgtttg gcgtagtcaa cgacaatccg gacatcatta
     9781 ctaacgttta tcgcgacgac gaatggttta tgaatcgcca tcgcccggaa gagatgcgct
     9841 tttttaaaga agcggtgttc aaatatgcgc tgtatgagcc gggattactg gagccggaag
     9901 gcgtcagcaa agtgtttttc acctgcgatt cccatgaaca actgctgccg ctggagcagg
     9961 cgattaacgc tcgctggggc gatcgcgtca acgtcagttt ctctacctta acctgtctgg
    10021 aagtgatggc gggcggcgtg tcgaaaggcc atgcgctgga agcagtggcg aagaaactgg
    10081 gctacagcct gaaggattgt attgcgtttg gtgacgggat gaacgacgcc gaaatgctgt
    10141 cgatggcggg gaaaggctgc attatgggca gtgcgcacca gcgtctgaaa gatctgcatc
    10201 cggagctgga agttattggt actaatgccg acgacgcggt gccgcattat ctgcgtaaac
    10261 tctatttatc gtaatcgttc tttatttggt cagttgtcaa cctgatactt cgctacaatg
    10321 gatactcgtt aatcaaagag ttttccattg tggcgttact tatcatcacc acgattctgt
    10381 gggccttctc ctttagcttt tatggcgagt accttgcggg gcacgtcgat agctattttg
    10441 cggtgctggt gcgcgttggc ttggcggcac tcgtttttct gccgtttctg cgtacccgta
    10501 gcaatagcct gaaaacggtc ggcctgtata tgctggtggg cgcgatgcag ctcggcgtga
    10561 tgtatatgct gagtttccgc gcttatctct acctgacggt ctccgaattg ttgctgttca
    10621 ccgtactgac gccgctctac atcacgctga tttatgacat catgagtaag cgccgtctgc
    10681 gctggggcta tgcttttagc gccttgctgg cggtgattgg tgccgggatt attcgctatg
    10741 atcaggtcac cgaccatttc tggactggct tgttgctggt gcaactctcc aatatcactt
    10801 ttgccatcgg catggtgggt tacaaacgcc tgatggaaac tcgcccgatg ccgcagtata
    10861 acgcctttgc gtggttctat cttggcgcgt tcctggtggc ggtaattgca tggttcttgc
    10921 tgggaaatgc gcagaaaatg ccgcaaacca cgttgcaatg gggcattctg gtgtttcttg
    10981 gcgtggtggc ttccgggatt ggctacttta tgtggaacta cggcgcgacg caggtggacg
    11041 ccggaacgct gggcattatg aataatatgc acgttccggc agggctgctg gtaaacctgg
    11101 ctatctggca ccaacagccg cactggccaa cgtttattac aggcgcgctg gtgatcctgg
    11161 cctcactgtg ggtgcatcgt aagtgggtcg ctccgcgctc ttcacaaacg gcagatgatc
    11221 gcaggcgtga ttgcgcgctg agcgaataaa cgcttccgta actggctgac gctgctcgcc
    11281 atcgcgcacg gcggcgtaca gtcggctcca caagccttcg cccagggttt tggtcaccac
    11341 cagaccctgg cgctcaaaac tctctactac ccaatgcggt agcgcggcaa tacccatccg
    11401 cgcggcaacc atttgaatca acaataaggt gttatcgacg cttttcagtg acgggctgac
    11461 gcctgccggc tgaagaaaat gccgccagac atccagtcga ctacgctgca ccggataaat
    11521 taatagcgtc tcgctggcga gatcttccgg tgtaatacgc gtttttgccg ccagtggatg
    11581 gtcaggtgct aacaccaggc gtacttcata atcgaacatc ggcgaataat gcaggccact
    11641 gcgcggcagg atatcggagg tcatcaccag atccagctcg ccctgttgca acgcgggctg
    11701 cgggtcaaac gtcacgcccg atttaaaatc catctctacc tgcggccagt tcttatggaa
    11761 attttctaaa gcgggtgtca gccactgaat acagctatgg cactcaatgg caatgcgcag
    11821 acgcgtctgc tgcggttcat tgcaggcttg cagtgcctgg ctgatttgcg gcagcacctg
    11881 gtttgccagt tgcaacagga tttctccctg cggtgtaaag cgtagcggct ggctcttacg
    11941 cacaaatagc cggaagccaa ggcgttgttc cagatcgcta aactggtgag acagggcgga
    12001 ttgcgtctga tgcaacgttg ccgcagcggc tgcgagcgag ccgcagttcc gcaacgcttg
    12061 tagcgttttc aggtgtttta cttcgatcat gaaagtcctt cacttcggca tgaataattt
    12121 gcgcttgagg aatatacagt aaccgccaat tatggatgtg taaacatctg gacggctaaa
    12181 atcattcgtc ttttaaattt atggtgcgtt ggctgcgttt ctccaccccg gtcacttact
    12241 tcagtaagct cccggcgatg aataaacttg ccgccttccc taaattcaaa atccatagga
    12301 tttacatata attagaggaa gaaaaatgac aatattgaat cacaccctcg gtttccctcg
    12361 cgttggcctg cgtcgcgagc tgaaaaaagc gcaagaaagt tattgggcgg ggaactccac
    12421 gcgtgaagaa ctgctgacag tagggcgtga actgcgtgct cgtcactggg atcaacaaaa
    12481 gcaagcgggt atcgacctgc tgccggtggg cgattttgcc tggtacgatc atgtactgac
    12541 caccagtttg ctgctgggta atgttccgcc acgtcatcag aacaaagacg gttcggtaga
    12601 tatcgacacc ctgttccgta ttggtcgtgg acgtgcgccg actggcgaac ctgcggcggc
    12661 agcggaaatg accaaatggt ttaacaccaa ctatcactac atggtgccgg agttcgttaa
    12721 aggccaacag ttcaaactga cctggacgca gctgctggag gaagtggacg aggcgctggc
    12781 gctgagccac aacgttaagc ctgtgctgct ggggccggtt acttacctgt ggctggggaa
    12841 agtgaaaggt gaacagtttg accgccttag cctgctgaac gacattctgc cggtttatca
    12901 gcaagtgctg gcagaactgg cgaaacgcgg catcgagtgg gtacagattg atgaacccgc
    12961 gctggtactg gaactaccac aggcgtggct ggacgcatac aaacccgctt acgacgcgct
    13021 ccagggacag gtgaaactgc tgctgaccac ctattttgaa ggcgtaacgc caaatctcga
    13081 cacgattact gcgctgcctg ttcaggggct gcatgtggac cttgtacatg gtaaagataa
    13141 cgttgttgaa ctgcacaagc gcctgccttc tgactggctg ctgtctgcgg gtctgatcaa
    13201 tggtcgtaac gtctggcgcg ccgatcttac tgagaaatat gcgcaaatta aggacattgt
    13261 cggcaaacgt gatttgtggg tggcatcttc ctgctcgttg ctgcacagcc ccatcgacct
    13321 gagcgtggaa acgcgtcttg atgcagaagt gaaaagctgg tttgccttcg ccctacaaaa
    13381 atgccatgaa ctggcactgc tgcgcgatgc gctgaacagt ggtgacacgg cagctctggc
    13441 agagtggagc gccccaattc aggcgcgtcg tcactctact cgtgtacata atccggcggt
    13501 agaaaagcgt ctggcggcga tcaccgccca ggacagccag cgtgcgaatg tctatgaagt
    13561 gcgtgctgaa gcccagcgtg cgcgttttaa actgccagcg tggccgacca ccacgattgg
    13621 ttccttcccg caaaccacgg aaattcgtac cctgcgtctg gatttcaaaa agggcaatct
    13681 cgacgccaac aactaccgca cgggcattgc ggaacatatc aagcaggcca ttgttgagca
    13741 ggaacgtttg ggactggatg tgctggtaca tggcgaggcc gagcgtaatg acatggtgga
    13801 atactttggc gagcacctcg acggatttgt ctttacgcaa aacggttggg tacagagcta
    13861 cggttcccgc tgcgtgaagc caccgattgt cattggtgac attagccgcc cggcaccgat
    13921 taccgtggag tgggcgaagt atgcgcaatc gctgaccgac aaaccggtga aagggatgct
    13981 gacggggccg gtgaccatac tctgctggtc gttcccgcgt gaagatgtca gccgtgaaac
    14041 catcgccaaa cagattgcgc tggcgctgcg tgatgaagtg gccgatctgg aagccgctgg
    14101 aattggcatc atccagattg acgaaccggc gctgcgcgaa ggtttaccgc tgcgtcgtag
    14161 cgactgggat gcgtatctcc agtggggcgt agaggccttc cgtatcaacg ccgccgtggc
    14221 gaaagatgac acacaaatcc acactcacat gtgttattgc gagttcaacg acatcatgga
    14281 ttcgattgcg gcgctggacg cagacgtcat caccatcgaa acctcgcgtt ccgacatgga
    14341 gttgctggag tcgtttgaag agtttgatta tccaaatgaa atcggtcctg gcgtctatga
    14401 cattcactcg ccaaacgtac cgagcgtgga atggattgaa gccttgctga agaaagcggc
    14461 aaaacgcatt ccggcagagc gcctgtgggt caacccggac tgtggcctga aaacgcgcgg
    14521 ctggccagaa acccgcgcgg cactggcgaa catggtgcag gcggcgcaga atttgcgtcg
    14581 ggggtaaatc caaaccgggt ggtaatacca cccggtcttt tctcattaca gcgacttctt
    14641 cccaccatac tgcttaaacc attccagcat acgctgccag ccatctttcg cagattcggc
    14701 atgatagctc gggcgataat cagcgttgaa tgcatgcccg gcatccgggt acacgataat
    14761 ctctgctttc gcattagcag cccgcagcgc ctggcgcatc gtttcaacgc tctcctgcgg
    14821 aatgctgtta tcctgaccac catataagcc gagaaccggc gcgttaagat cggttgcgat
    14881 atcaacaggt tgtttcggtg aattcagcga cttgtcgccc gtcagtttgc cgtaccacgc
    14941 cactgcggct tttagctgtg gattatgcgc ggcatacagc caggtgatac gtccacccca
    15001 gcagaatccg gtgatcatta aacgatgaac atcgccgccg ttgcgggaag cccaactggc
    15061 gacatgatcg agatcggcca gcacctgcga gtcaggcact tttgctacca gaccgctaag
    15121 caacgtgggg atatcggcaa aatcattcgg atcgccttcg cggaagtaaa gttcaggtgc
    15181 gatagccaga tacccctcca gcgccagacg gcgacaaatg tcgcggatat gttcatgcac
    15241 gccaaaaatt tcctgcacta caatgaccac tggcagtggg ccatcgcttt gctttggtct
    15301 ggcatggtaa gcaggcatgt tatccccttg tgaagggata gaggtgaagc ccgccacaat
    15361 cgcgtcgtcc ggggtctgaa cgatggtcga agcgagaggc gatgcagcag gtgcaaatcc
    15421 agattgttgt gttgttgcca tggtattctc cgtaccctta taaaaatgtt gcgcaatgtt
    15481 aactatagtc agcatgcaac aaatcacatt gcctgaatcg gctcatcttt tatgcagtcc
    15541 tgcagaatga agagtgattt atgtgatatg aatcactttt ggtgggtaaa tttatgcaac
    15601 gcatttgcgt catggtgatg agtatcacga aaaaatgtta aacccttcgg taaagtgtct
    15661 ttttgcttct tctgactaaa ccgattcaca gaggagttgt atatgtccaa gtctgatgtt
    15721 tttcatctcg gcctcactaa aaacgattta caaggggcta cgcttgccat cgtccctggc
    15781 gacccggatc gtgtggaaaa gatcgccgcg ctgatggata agccggttaa gctggcatct
    15841 caccgcgaat tcactacctg gcgtgcagag ctggatggta aacctgttat cgtctgctct
    15901 accggtatcg gcggcccgtc tacctctatt gctgttgaag agctggcaca gctgggcatt
    15961 cgcaccttcc tgcgtatcgg tacaacgggc gctattcagc cgcatattaa tgtgggtgat
    16021 gtcctggtta ccacggcgtc tgtccgtctg gatggcgcga gcctgcactt cgcaccgctg
    16081 gaattcccgg ctgtcgctga tttcgaatgt acgactgcgc tggttgaagc tgcgaaatct
    16141 attggcgcga caactcacgt tggcgtgaca gtttcttctg ataccttcta cccaggtcag
    16201 gaacgttacg atacttactc tggtcgcgta gttcgtcact ttaaaggttc tatggaagag
    16261 tggcaggcga tgggcgtaat gaactatgaa atggaatctg caactctgct gaccatgtgt
    16321 gcaagtcagg gcctgcgtgc cggtatggta gcgggtgtta tcgttaaccg cacccagcaa
    16381 gagatcccga atgctgagac gatgaaacaa accgaaagcc atgcggtgaa aatcgtggtg
    16441 gaagcggcgc gtcgtctgct gtaattctct tctcctgtct gaaggccgac gcgttcggcc
    16501 ttttgtattt ttgcgtagcg cctcgcagga aatgcctttc caactggacg tttgtacagc
    16561 acaattctat tttgtgcggg taagttgttg cgtcaggagg cgttgtggat ttctcaatca
    16621 tggtttacgc agttattgcg ttggtgggtg tggcaattgg ctggctgttt gccagttatc
    16681 aacatgcgca gcaaaaagcc gagcaattag ctgaacgtga agagatggtc gcggagttaa
    16741 gcgcggcaaa acaacaaatt acccaaagcg agcactggcg tgcagagtgt gaattgctca
    16801 ataacgaagt gcgcagcctg caaagtatta acacctctct ggaggccgat ctgcgtgaag
    16861 taaccacgcg gatggaagcc gcacagcaac atgctgacga taaaattcgc cagatgatta
    16921 acagcgagca gcgcctcagt gagcagtttg aaaacctcgc caaccgtatt tttgagcaca
    16981 gcaatcgccg ggttgatgag caaaaccgtc agagtctgaa cagcctgttg tcgccgctac
    17041 gtgaacaact ggacggtttc cgccgtcagg ttcaggacag cttcggtaaa gaagcacaag
    17101 aacgccatac cctgacccac gaaattcgca atctccagca actcaacgcg caaatggccc
    17161 aggaagcgat caacctgacg cgcgcgctga aaggcgacaa taaaacccag ggcaactggg
    17221 gcgaggtagt attgacgcgg gtgctggagg cttccggtct gcgtgaaggg tatgaatatg
    17281 aaacccaggt cagcatcgaa aatgacgccc gctcgcggat gcagccggat gtcatcgtgc
    17341 ggctgccgca gggaaaagat gtggtgatcg acgccaaaat gacgctggtc gcctatgaac
    17401 gctattttaa cgccgaagac gactacaccc gcgaaagcgc gctacaggaa catatcgcgt
    17461 cggtgcgtaa ccatatccgt ttgctgggac gcaaagatta tcaacagctg ccggggctgc
    17521 gaactctgga ttacgtgctg atgtttattc ccgttgaacc cgctttttta ctggcgcttg
    17581 accgccagcc ggagctgatc accgaagcgt tgaaaaacaa tatcatgctg gttagcccga
    17641 ctacgctgct ggtggcgttg cgtactattg ccaacctgtg gcgttatgag catcaaagcc
    17701 gtaacgccca gcaaatcgcc gatcgtgcca gcaagctgta cgacaagatg cgtttgttca
    17761 tcgatgacat gtccgcgatt ggtcaaagtc tcgacaaagc gcaggataac taccgccagg
    17821 caatgaaaaa actctcttca ggtcgcggaa atgtgctggc gcaggcagaa gcgtttcgcg
    17881 gtttaggagt agaaattaaa cgcgagatta atccggattt ggctgaacag gcggtgagcc
    17941 aggatgaaga gtatcgactt cggtcggttc cggagcagcc gaatgatgaa gcttatcaac
    18001 gcgatgatga atataatcag cagtcgcgct agcccattag gggtagttaa gccgggtaga
    18061 aatctagggc atcgacgccc aatctgttac acttctggaa caattttttg atgagcaggc
    18121 attgagatgg tggataagtc acaagaaacg acgcactttg gttttcagac cgtcgcgaag
    18181 gaacaaaaag cggatatggt cgcccacgtt ttccattccg tggcatcaaa atacgatgtc
    18241 atgaatgatt tgatgtcatt tggtattcat cgtttgtgga agcgattcac gattgattgc
    18301 agcggcgtac gccgtgggca gaccgtgctg gatctggctg gtggcaccgg cgacctgaca
    18361 gcgaaattct cccgcctggt cggagaaact ggcaaagtgg tccttgctga tatcaatgaa
    18421 tccatgctca aaatgggacg cgagaagctg cgtaatatcg gtgtgattgg caacgttgag
    18481 tatgttcagg cgaacgctga ggcgctgccg ttcccggata acacctttga ttgcatcacc
    18541 atttcgtttg gtctgcgtaa cgtcaccgac aaagataaag cactgcgttc aatgtatcgc
    18601 gtgctgaaac ccggcggccg cctgctggtg cttgagttct cgaagccaat tatcgagccg
    18661 ctgagcaaag cctatgatgc atactccttc catgtgctgc cgcgtattgg ctcactggtc
    18721 gcgaacgacg ccgacagcta ccgttatctg gcagaatcca tccgtatgca tcccgatcag
    18781 gataccctga aagccatgat gcaggatgcc ggattcgaaa gtgtcgacta ctacaatctg
    18841 acggcagggg ttgtggcgct gcatcgtggt tataagttct gacaggagac cggaaatgcc
    18901 ttttaaacct ttagtgacgg caggaattga aagtctgctc aacaccttcc tgtatcgctc
    18961 acccgcgctg aaaacagccc gctcgcgtct gctgggtaaa gtattgcgcg tggaggtaaa
    19021 aggcttttcg acgtcattga ttctggtgtt cagcgaacgc caggttgatg tactgggcga
    19081 atgggcaggc gatgctgact gcaccgttat cgcctacgcc agtgtgttgc cgaaacttcg
    19141 cgatcgccag cagcttaccg cactgattcg cagtggtgag ctggaagtgc agggcgatat
    19201 tcaggtggtg caaaacttcg ttgcgctggc agatctggca gagttcgacc ctgcggaact
    19261 gctggcccct tataccggtg atatcgccgc tgaaggaatc agcaaagcca tgcgcggagg
    19321 cgcaaagttc ctgcatcacg gcattaagcg ccagcaacgt tatgtggcgg aagccattac
    19381 tgaagagtgg cgtatggcac ccggtccgct tgaagtggcc tggtttgcgg aagagacggc
    19441 tgccgtcgag cgtgctgttg atgccctgac caaacggctg gaaaaactgg aggctaaatg
    19501 acgccaggtg aagtacggcg cctatatttc atcattcgca cttttttaag ctacggactt
    19561 gatgaactga tccccaaaat gcgtatcacc ctgccgctac ggctatggcg atactcatta
    19621 ttctggatgc caaatcggca taaagacaaa cttttaggtg agcgactacg actggccctg
    19681 caagaactgg ggccggtttg gatcaagttc gggcaaatgt tatcaacccg ccgcgatctt
    19741 tttccaccgc atattgccga tcagctggcg ttattgcagg acaaagttgc tccgtttgat
    19801 ggcaagctgg cgaagcagca gattgaagct gcaatgggcg gcttgccggt agaagcgtgg
    19861 tttgacgatt ttgaaatcaa gccgctggct tctgcttcta tcgcccaggt tcataccgcg
    19921 cgattgaaat cgaatggtaa agaggtggtg attaaagtca tccgcccgga tattttgccg
    19981 gtcattaaag cggatctgaa acttatctac cgtctggctc gctgggtgcc gcgtttgctg
    20041 ccggatggtc gccgtctgcg cccaaccgaa gtggtgcgcg agtacgaaaa gaccttgatt
    20101 gatgaactga atttgctgcg ggaatctgcc aacgccattc agcttcggcg caattttgaa
    20161 gacagcccga tgctctacat cccggaagtt taccctgact attgtagtga agggatgatg
    20221 gtgatggagc gcatttacgg cattccggtg tctgatgttg cggcgctgga gaaaaacggc
    20281 actaacatga aattgctggc ggaacgcggc gtgcaggtgt tcttcactca ggtctttcgc
    20341 gacagctttt tccatgccga tatgcaccct ggcaacatct tcgtaagcta tgaacacccg
    20401 gaaaacccga aatatatcgg cattgattgc gggattgttg gctcgctaaa caaagaagat
    20461 aaacgctatc tggcagaaaa ctttatcgcc ttctttaatc gcgactatcg caaagtggca
    20521 gagctacacg tcgattctgg ctgggtgcca ccagatacca acgttgaaga gttcgaattt
    20581 gccattcgta cggtctgtga acctatcttt gagaaaccgc tggccgaaat ttcgtttgga
    20641 catgtactgt taaatctgtt taatacggcg cgtcgcttca atatggaagt gcagccgcaa
    20701 ctggtgttac tccagaaaac cctgctctat gtcgaagggg taggacgcca gctttatccg
    20761 cagctcgatt tatggaaaac agcgaagcct ttcctggagt cgtggattaa agatcaggtc
    20821 ggtattcctg ccctggtgag agcatttaaa gaaaaagcgc cgttctgggt cgaaaaaatg
    20881 ccagaactgc ctgaactggt ttacgacagt ttgcgccagg gcaagtattt acagcacagt
    20941 gttgataaga ttgcccgcga gcttcagtca aatcatgtac gtcagggaca atcgcgttat
    21001 tttctcggaa ttggcgctac gttagtatta agtggcacat tcttgttggt cagccgacct
    21061 gagtgggggc tgatgcccgg ctggttaatg gcaggtggtc tgatcgcctg gtttgtcggt
    21121 tggcgcaaaa cacgctgatt ttttcatcgc tcaaggcggg ccgtgtaacg tataatgcgg
    21181 ctttgtttaa tcatcatcta ccacagagga acatgtatgg gtggtatcag tatttggcag
    21241 ttattgatta ttgccgtcat cgttgtactg ctttttggca ccaaaaagct cggctccatc
    21301 ggttccgatc ttggtgcgtc gatcaaaggc tttaaaaaag caatgagcga tgatgaacca
    21361 aagcaggata aaaccagtca ggatgctgat tttactgcga aaactatcgc cgataagcag
    21421 gcggatacga atcaggaaca ggctaaaaca gaagacgcga agcgccacga taaagagcag
    21481 gtgtaatccg tgtttgatat cggttttagc gaactgctat tggtgttcat catcggcctc
    21541 gtcgttctgg ggccgcaacg actgcctgtg gcggtaaaaa cggtagcggg ctggattcgc
    21601 gcgttgcgtt cactggcgac aacggtgcag aacgaactga cccaggagtt aaaactccag
    21661 gagtttcagg acagtctgaa aaaggttgaa aaggcgagcc tcactaacct gacgcccgaa
    21721 ctgaaagcgt cgatggatga actacgccag gccgcggagt cgatgaagcg ttcctacgtt
    21781 gcaaacgatc ctgaaaaggc gagcgatgaa gcgcacacca tccataaccc ggtggtgaaa
    21841 gataatgaag ctgcgcatga gggcgtaacg cctgccgctg cacaaacgca ggccagttcg
    21901 ccggaacaga agccagaaac cacgccagag ccggtggtaa aacctgctgc ggacgctgaa
    21961 ccgaaaaccg ctgcaccttc cccttcgtcg agtgataaac cgtaaacatg tctgtagaag
    22021 atactcaacc gcttatcacg catctgattg agctgcgtaa gcgtctgctg aactgcatta
    22081 tcgcggtgat cgtgatattc ctgtgtctgg tctatttcgc caatgacatc tatcacttgg
    22141 tatccgcgcc attgatcaag cagttgccgc aaggttcaac gatgatcgcc accgacgtgg
    22201 cctcgccgtt ctttacgccg atcaagctga cctttatggt gtcgctgatt ctgtcagcgc
    22261 cggtgattct ctatcaggtg tgggcattta tcgccccagc gctgtataag catgaacgtc
    22321 gcctggtggt gccgctgctg gtttccagct ctctgctgtt ttatatcggc atggcattcg
    22381 cctactttgt ggtctttccg ctggcatttg gcttccttgc caataccgcg ccggaagggg
    22441 tgcaggtatc caccgacatc gccagctatt taagcttcgt tatggcgctg tttatggcgt
    22501 ttggtgtctc ctttgaagtg ccggtggcaa ttgtgctgct gtgctggatg gggattacct
    22561 cgtcagaaga cttacgcaaa aaacgcccgt atgtgctggt tggtgcattc gttgtcggga
    22621 tgttgctgac gccgccggat gtcttctcgc aaacgctatt ggcgatcccg atgtactgtc
    22681 tgtttgaaat cggtgtcttc ttctcacgct tttacgttgg taaagggcga aatcgggaag
    22741 aggaaaacga cgctgaagca gaaagcgaaa aaactgaaga ataaattcaa ccgcccgtca
    22801 gggcggttgt catatggagt acaggatgtt tgatatcggc gttaatttga ccagttcgca
    22861 atttgcgaaa gaccgtgatg atgttgtagc gcgcgctttt gacgcgggag ttaatgggct
    22921 actcatcacc ggcactaacc tgcgtgaaag ccagcaggcg caaaagctgg cgcgtcagta
    22981 ttcgtcctgt tggtcaacgg cgggcgtaca tcctcacgac agcagccagt ggcaagctgc
    23041 gactgaagaa gcgattattg agctggccgc gcagccagaa gtggtggcga ttggtgaatg
    23101 tggtctcgac tttaaccgca acttttcgac gccggaagag caggaacgcg cttttgttgc
    23161 tcagctacgc attgccgcag aattaaacat gccggtattt atgcactgtc gcgatgccca
    23221 cgagcggttt atgacattgc tggagccgtg gctggataaa ctgcctggtg cggttcttca
    23281 ttgctttacc ggcacacgcg aagagatgca ggcgtgcgtg gcgcgtggaa tttatatcgg
    23341 cattaccggt tgggtttgcg atgaacgacg cgggctggag ctgcgggaat tgttgccgtt
    23401 gattccggcg gagaaattgc tgatcgaaac agatgcgccg tatctgctcc cgcgcgatct
    23461 cacgccaaag ccatcatccc ggcgcaacga gccagcccat ctgccccata ttttgcaacg
    23521 tattgcgcac tggcgtggag aagatgccgc atggctggct gccaccacgg atgccaatgt
    23581 caaaacactg tttgggattg cgttttagag tttgcggaac tcggtgttct tcacactgtg
    23641 cttaatctct ttattaataa gattaagcaa tagcatggag cgagcctcac catcgggttc
    23701 ggtgaaaatg gcctgaaagc cttcgaacgc gccttcggta ataatcacct tatcacccgg
    23761 ataagggggg gccggatcga caatgtcttt cggtttatat accgatagct gatgaattac
    23821 cgccgatggg actatcgctg gcgacgcgcc aaagcgcaca aagtggctga cgccacgcgt
    23881 agcgctgatg gtcgtggtat gaatcacttc tggatcaaat tccacaaaca ggtagttagg
    23941 gaataacggt tcactgactg cggtacgttt accccgtacg attttttcca gggtgatcat
    24001 cggagcgagg caattaaccg cctgtctctc aaggtgttcc tgggcacgtt gaagttgccc
    24061 gcgcttgcag tacagtaaat accaggattg cataatgact cttatccgtt taatcggggc
    24121 gcaaggatag caaaagcttt acgctaagtt aattatattc cccggtttgc gttataccgt
    24181 cagagttcac gctaatttaa caaatttaca gcattgcaaa gatgaacgcc gtataatggg
    24241 cgcagattaa gaggctacaa tggacgccat gaaatataac gatttacgcg acttcctgac
    24301 gctgcttgaa cagcagggtg agctaaaacg tatcacgctc ccggtggatc cgcatctgga
    24361 aatcactgaa attgctgacc gcactctgcg tgctggtggg cctgcgctgt tgttcgaaaa
    24421 ccctaaaggc tactcaatgc cggtgctgtg caacctgttc ggtacgccaa agcgcgtggc
    24481 gatgggcatg gggcaggaag atgtttcggc gctgcgtgaa gttggtaaat tattggcgtt
    24541 tctgaaagag ccggagccgc caaaaggttt ccgcgacctg tttgataaac tgccgcagtt
    24601 taagcaggtg ttaaacatgc cgacaaagcg actgcgtggt gcgccctgcc aacaaaaaat
    24661 cgtctctggc gatgacgtcg atctcaatcg cattcccatt atgacctgct ggccggaaga
    24721 tgccgcgccg ctgattacct gggggctgac cgtaacgcgc ggcccgcata aagagcggca
    24781 gaatctgggc atttatcgcc agcagctgat tggtaaaaac aaactgatta tgcgctggct
    24841 gtcgcatcgc ggcggcgcgc tggattatca ggagtggtgt gcggcgcatc cgggcgaaca
    24901 tttcccggtt tctgtggcgc tgggtgccga tcctgccacg attctcggtg cagtcacccc
    24961 cgttccggat acgctttcag agtatgcgtt tgccggattg ctacgcggca ccaaaaccga
    25021 agtagtaaag tgtatttcca atgacctcga agtgccagcc agtgccgaaa tcgtcctgga
    25081 agggtatatc gatcctggtg agatggcgcc ggaagggccg tatggtgacc acacaggtta
    25141 ctataatgaa gtcgatagtt tcccggtgtt taccgtgacg catattaccc agcgtgaaga
    25201 tgcgatttac cattccacct ataccgggcg tccgccagat gagcccgcgg tactgggcgt
    25261 ggcgttgaac gaagtgtttg taccgattct gcaaaaacag ttcccggaaa ttgtcgattt
    25321 ttacctgccg ccggaaggct gttcttatcg tctggcggta gtgacgatca aaaaacagta
    25381 cgccggacac gcgaagcgcg tcatgatggg cgtctggtcg ttcttacgcc agtttatgta
    25441 cactaaattt gtgatcgttt gcgatgatga cgtcaacgca cgcgactgga acgatgtgat
    25501 ttgggcgatt accacccgta tggacccggc gcgggatact gttctggtag aaaatacgcc
    25561 tattgattat ctggattttg cctcgcctgt ctctgggctg ggttcaaaaa tggggctgga
    25621 tgccacgaat aaatggccgg gggaaaccca gcgtgaatgg ggacgtccca tcaaaaaaga
    25681 tccagatgtt gtcgcgcata ttgacgccat ctgggatgaa ctggctattt ttaacaacgg
    25741 taaaagcgcc tgatgcgcgt ttgttttgcc ctatttatcg atccgacaga gaaagcgcat
    25801 gacaacctta agctgtaaag tgacctcggt agcagctatc acggataccg tatatcgtgt
    25861 ccgcatcgtg ccagacgcgg ccttttcttt tcgtgctggt cagtatttga tggtagtgat
    25921 ggatgagcgc gacaaacgtc cgttctcaat ggcctcgacg ccggatgaaa aagggtttat
    25981 cgagctgcat attggcgctt ctgaaatcaa cctttacgcg aaagcagtca tggaccgcat
    26041 cctcaaagat catcaaatcg tggtcgacct tccccacgga gaagcgtggc tgcgcgatga
    26101 tgaagagcgt ccgatgattt tgattgcggg cggcaccggg ttctcttatg cccgctcgat
    26161 tttgctgaca gcgttggcgc gtaacccaaa ccgtgatatc accatttact ggggcgggcg
    26221 tgaagagcag catctgtatg atctctgcga gcttgaggcg ctttcgttaa agcaccttgg
    26281 tctgcaagtg gtgccggtgg ttgaacaacc ggaagcaggc tggcgtgggc gtactggtac
    26341 cgtgttaacg gcggtattgc aggatcatgg tacgctggca gagcatgata tctatattgc
    26401 cggacgtttt gagatggcga aaattgcccg tgacctgttt tgcagtgagc gtaatgcgcg
    26461 ggaagatcgc ctgtttggcg atgcgtttgc atttatctga gatataaaaa aacccgcccc
    26521 tgacaggcgg gaagaacggc aactaaaact ttattcagtg gcatttagat ctatgtcgca
    26581 tccggcaatt ggtgcacgat gcctgatgcg acgctggcgc gtcttatcag gcctacattg
    26641 gtgccggatc ggtagaccgg ataaggcgtt cacgccgcat ccggcaagtg gttaaacccg
    26701 ctcaaacacc gtcgcaatac cctgacccag accgatacac atcgtcgcca gaccaaactg
    26761 aacgtctttg cgttccatca gattcagcag cgtggtgctg atacgcgcac cggaacaacc
    26821 cagcggatga cccagcgcga tcgcaccacc attgaggttg atcttctcgt caatctgctc
    26881 cattagcccg agatctttaa tgcacggcag gatctgcgcg gcaaaggctt cgttcatctc
    26941 aaacacgccg atatcgctgg cagaaagccc cgcttttttc agcgccagct tcgaggccgg
    27001 aaccgggccg taacccataa tcgatgggtc acaaccaacg accgccatcg aacgcacacg
    27061 agcgcgcggc ttaagaccta attcatgggc gcggctttca ctcatcacca gcatggcagc
    27121 tgcgccatcg gaaagtgcag aagatgtgcc cgccgttacc gtaccgttta ctggatcaaa
    27181 cgccggacgc agcgtggcga gggcttccac ggtggtttcc gggcgaatca cttcgtcgta
    27241 attaaactgc ttcaggacgc cgtcggcatc gtgaccacca gttgggatga tttcattttt
    27301 aaatgcggcc gactgcgtgg cggcccaggc gcgggcgtgt gaccgcgcgg caaaggcatc
    27361 ctgcatttca cggctgatac cgtgcatacg cgccagcatt tctgccgtta agcccatcat
    27421 gcccgccgct ttggcgacat tgcggctcag gccggggtga aaatcgacgc cgtgactcat
    27481 cggcacatgg cctatatgct ccacgccgcc aaccagacat gcctgcgcat cgccagtcat
    27541 gatcattcgt gctgcgtcat gcagtgcctg catggatgaa ccacacaagc gattgacggt
    27601 aaccgccggg acagagtgcg gcacttctgc cagcagcgcc gcgttacggg cgatgttaaa
    27661 accctgctcc agcgtctgtt gcacacaacc ccagtaaata tcgtcgaggg ccgccgcttc
    27721 cagcgccggg ttacgcgcca gcaggctacg cattaaatga gcggagagat cttccgcacg
    27781 cacgttacga aaagcaccgc ccttcgaacg gcccatcggg gtgcgaattg catcgacaat
    27841 gacaacctgt tccattgtga ctccttaagc cgttttcagg tcgccaaccg gacgggctgg
    27901 ctcaaccgga ggatagtacg gttcgttatg acgcgcttta ttacgcagac cttccggcac
    27961 ttcatacagc gggccgaggt gctgatattg ctgtgccata tcgaggtatt ttgcgctacc
    28021 gagggtgtcc agccagcgga acgcgccgcc gtggaacgga gggaagccca ggccgtagac
    28081 cagcgccata tccgcttccg ccggagtggc gataatgccc tcttccagac agcgcaccac
    28141 ttcgttgacc atcgggatca tcatgcgggc gataatctct tcttcgctga aatcgcgctt
    28201 cggttggctc acttctgcca gcaggtcttc aacggcggcg tcttcttctt tcttcggctt
    28261 acctttgctg tcttctttat aacgccagaa accgaggccg ttcttctgac caaagcggtt
    28321 ggcatcaaac agcgcgtcga tggcatcgcg gtaatctttc tgcatccgct gcgggaagcc
    28381 tgctgccatg acagcctgag cgtgatgcgc ggtatcaatg cccacaacgt ccagcagata
    28441 tgccgggccc atcggccagc caaactgttt ttccatcact ttgtcgatct tgcggaaatc
    28501 cgcgccgtcg cgcagcagct ggctgaaacc ggcgaaatac gggaacagca cgcggttaac
    28561 aaagaagccg gggcagtcgt taaccacaat cggcgtcttg cccatcttgc tcgcccaggc
    28621 gacaactttc gcgatggttt cgtcggagct tttctcgccg cgaataattt ctaccaacgg
    28681 cattcggtgg accgggttaa agaagtgcat cccgcagaag ttttccgggc gttccagcgc
    28741 gttggccagt tcgctgatag gaatggttga agtgttagac gccagcacgg tatcctggcg
    28801 tactttttgt tcggtttctg ccagtacggc ttttttcact ttcgggtttt caacaaccgc
    28861 ttctaccaca atatccacgc ggtcaaatcc ggcgtagtcg agcgttgggt ggatcgtgga
    28921 gatcacgcca gccagtttca gaccatcgat tttgccgcgc tcaagctgct tgttcagcag
    28981 tttcgcggct tcggtcatgc cgagggttaa cgacttgtcg ttgatatctt tcatgacaac
    29041 cggcacgcct ttccacgcgg actggtaagc gataccgcca cccatgatgc ctgcgcccag
    29101 cacagcagcc tgtttcgggg tttcaacgtc tttggtgagt ttcttcgctt tgccttttac
    29161 atattgatcg ttaaggaaaa tgccgaccag tgcgcgagct tcgttggtat gcgccagcgg
    29221 gacaaaactt ttgttttcca gatttaaggc ttcttcacga ccaaaacggg ccgcagcttc
    29281 aatggttttt actgcggtga tgggggccgg ataatgtttc cccgccgttt gtgcgaccat
    29341 tcctttagcg atggtgaagc tcatggtggc ttcaatcttg ctcagtttta gcggttcctg
    29401 cttcggctga cgtttcgctt tccagtcgag gtcgccgtta atggcctggc gtaaaatcgc
    29461 catcgcccct tcaatcagtt tttctgcttt gactacgcca tccaccagac cgattttcag
    29521 cgcctgatcc gcgccgacat ctttaccggc ggcaatgatt tccagtgcac tgtcagcgcc
    29581 cagcatacgt ggcatacgta cagaaccgcc aaagcccggc atgatgccca gtttggtttc
    29641 cggcagaccg atgcgcagat ccggcgtcgc cagacgataa tcggtcgcca gcacgcattc
    29701 gcagccacca cccagcgcat agccgttgac ggcagcaatg gtcggcaccg gcagatcttc
    29761 caggcgatta aacacgctat tggcaaagtg cagccactga cttaactgtt cttcaggaac
    29821 gaggaacagg gacaaaaatt cggtgatatc agcaccgacg ataaaggctg ctttgttcga
    29881 acgcagcagc agccctttta gatctgattg ctgttccagc acgccgatgg cctcgccgag
    29941 gctggcgacg gtcgcagtgt cgagtttatt aactgaacct ggggcatcaa ataccagttc
    30001 ggcaatgcca tcttccagcc agtcaaggta cagggtgtcg cctttgtaaa gcatgtcagt
    30061 ctcctgaatc cgcaaggtga tctggtcgta ccagatgcat cggagtgtgt gttttgtgtt
    30121 aaaaatatgc aaataaaaga ttaaagaaat gccgatctga tcacgctcgg cagaaatcac
    30181 gctctggatg aacgatgtgc taagatgcgg agacttaagg tcaaaaaaac agaagggtaa
    30241 aaaatggaat cactggcctc gctctataaa aatcatatag ctaccttaca agaacggact
    30301 cgcgatgcgc tggcgcgctt caagctggat gcattactta ttcactccgg cgagctgttc
    30361 aacgtttttc tcgacgatca tccctatccg tttaaagtga acccgcaatt caaagcgtgg
    30421 gtgccggtaa ctcaggtgcc aaactgctgg ttgctggtgg atggcgtgaa caagccgaaa
    30481 ctgtggttct atctgccggt tgattactgg cacaacgtcg aaccgctgcc gacctccttc
    30541 tggactgaag atgtggaagt gatcgcgctg ccgaaagccg atggcattgg tagcctgctg
    30601 cctgctgcgc gcggcaatat cggttatatc ggtccggtgc cggaacgtgc gctgcaactg
    30661 ggtattgagg ccagcaacat caacccgaaa ggggtgatcg actacctgca ttattaccgc
    30721 tccttcaaaa ccgagtacga actggcctgt atgcgtgaag cgcagaaaat ggcggtcaat
    30781 ggtcatcgtg cggcagaaga agcgttccgt tctggcatga gcgagttcga tatcaatatt
    30841 gcctatctga ccgcgaccgg tcatcgtgat accgacgtac cttacagcaa cattgtggcg
    30901 cttaacgaac acgctgcggt gttgcattac accaaactgg accaccaggc accggaagag
    30961 atgcgcagct tcctgctgga tgccggggcc gaatataacg gctatgcggc tgacctgacc
    31021 cgtacctggt cggcaaaaag cgacaacgac tacgcacagc tggtgaaaga cgtaaatgat
    31081 gaacaactgg cgctgatcgc caccatgaaa gcaggcgtca gctatgtgga ttaccacatc
    31141 cagttccatc agcgcatcgc caaactgctg cgtaaacatc aaatcatcac cgatatgagt
    31201 gaagaagcga tggtcgaaaa cgatctcacc ggaccgttta tgccgcacgg tattggccat
    31261 ccgctgggcc tgcaggtgca tgacgtcgcc ggttttatgc aggatgatag cggtacacac
    31321 ctcgcggcac cggcaaaata tccgtacctg cgctacaccc gtattctcca gccgggcatg
    31381 gtgttaacca tcgaaccggg tatctacttc attgaatcgc ttctggcacc gtggcgtgaa
    31441 gggcagttca gcaagcactt caactggcag aaaattgaag cactgaaacc gttcggcggc
    31501 attcgtatcg aagacaacgt ggtgatccac gaaaacaacg tggaaaacat gacccgggat
    31561 ctgaaactgg cgtgatggaa agctggttaa ttcctgcggc accggtcacg gtcgttgaag
    31621 agatcaaaaa gagccgcttc ataacgatgt tggcgcatac cgatggcgtt gaggcggcga
    31681 aagcgtttgt tgaatcggtg cgggcagaac accccgatgc ccgccaccat tgcgtggcgt
    31741 gggtggcggg tgcgccggat gattctcaac agctgggttt ctctgacgac ggggagccgg
    31801 cgggaacggc aggtaaaccg atgctcgccc agctaatggg cagcggcgtc ggggaaatta
    31861 ccgctgtggt agtgcgctac tacggcggca tattgcttgg caccggtggg ttagtgaaag
    31921 cgtatggcgg cggcgtgaat caggcgctgc gccagctaac gacccaacgc aagacgccat
    31981 taaccgaata tactttgcaa tgtgaatata gtcagttaac cggcattgaa gcgttgctgg
    32041 ggcagtgtga cggcaaaatt atcaacagtg attatcaggc attcgttctg ctgcgggtgg
    32101 cgcttccggc ggcgaaagtg gctgaatttt ccgcaaagct ggcggatttt agccgtggtt
    32161 cattgcaatt gttagcgatt gaagaataat ccccacttcg ttttgcagaa ctaaggaagc
    32221 ggcagagatg cattttcgcg ccattacccg aatcgttgga ctactggtca tcttattttc
    32281 agggaccatg attatccctg ggctggtagc actcatctac cgggatggag cgggccgcgc
    32341 ttttacccag accttttttg tcgccctcgc cattggctct atgctgtggt ggccgaaccg
    32401 caaagagaag ggcgaactga aatcccgtga ggggtttctg atagtggtgc tgttttggac
    32461 cgtgctgggt agtgtcggtg cgctcccttt tatcttctcg gaaagcccga acctcacgat
    32521 taccgatgcg ttttttgaat ctttctctgg cctgaccacc accggggcca ctacgctggt
    32581 ggggctggat tcgctccctc atgccatcct cttttatcgc cagatgctgc aatggtttgg
    32641 cgggatgggg atcatcgtgt tagcggttgc gatactgcct atcctcggcg tgggtgggat
    32701 gcagctctat cgcgcagaaa tgcccggccc gctgaaagat aacaaaatgc gcccgcgaat
    32761 tgcggaaacg gcgaaaactc tgtggttgat ttatgtcttg ctgaccgtcg cctgtgcgct
    32821 ggcgttgtgg tttgccggaa tggatgcctt tgatgccatc ggccatagct ttgcgactat
    32881 cgctattggc ggcttctcga cacatgatgc cagtatcggt tatttcgaca gtccgactat
    32941 taacactatc attgctatct tcctgctgat ctccggctgt aactacggtc tgcacttttc
    33001 actgttaagt gggcgtagtc tgaaggttta ttggcgcgat ccggaatttc gcatgtttat
    33061 cggcgtacag tttacgctgg tggttatttg tacactcgta ctgtggtttc ataatgtcta
    33121 cagttcggcg ctgatgacaa ttaaccaggc gtttttccag gtggtatcga tggcgacaac
    33181 cgccgggttt acgactgaca gcattgcccg ctggccgctc tttttgccgg tactgctttt
    33241 atgttcagct tttatcggcg gttgcgccgg gtcaacgggc ggtggcctga aagtgatccg
    33301 catcctgctg ctgtttaagc aggggaaccg tgagctgaaa cgactggtgc atccgaacgc
    33361 cgtctatagc attaagctgg ggaatcgcgc actgccggaa cgtatcctcg aagccgtttg
    33421 gggatttttc tccgcctatg cattggtgtt tattgtcagt atgctggcga ttatcgccac
    33481 gggcgtggat gatttttctg cctttgcctc agttgttgcg acattgaata acctggggcc
    33541 agggcttggc gtggttgctg ataactttac cagtatgaac ccggtggcta aatggatcct
    33601 gattgccaac atgctgtttg gtcgtctcga ggtctttaca ttactggtgc tctttacccc
    33661 gactttctgg cgtgaatgat ggagtaatac gtgaaaacat taattctttt ctcaacaagg
    33721 gacggacaaa cgcgcgagat tgcctcctac ctggcttcgg aactgaaaga actggggatc
    33781 cagacggatg tcgccaatgt gcaccgcatt gaagaaccac aatgggaaaa ctatgaccgt
    33841 gtggtcattg gtgcttctat tcgctatggt cactaccact ctgcgttcca ggaatttgtc
    33901 aaaaaacatg cgacgcgact gaattcgatg ccgagcgcct tttattccgt aaacctggtg
    33961 gcgcgcaaac cggagaagcg tactccacag accaacagct acgcgcggaa gtttctgatg
    34021 aactcgcaat ggcgtcccga tcgctgcgcg gtcattgccg gggcgctgcg ttacccacgt
    34081 tatcgctggt acgaccgttt tatgctcaag ctgattatga agatgtcagg cggtgaaacg
    34141 gatacgcgca aagaagttgt ctataccgat tgggagcagg tggcgaattt cgcccgagaa
    34201 atcgcccatt taaccgacaa accgacgctg aaataagcat aaagaataaa aaatgcgcgg
    34261 tcagaaaatt attttaaatt tcctcttgtc aggccggaat aactccctat aatgcgccac
    34321 cactgacacg gaacaacggc aaacacgccg ccgggtcagc ggggttctcc tgagaattcc
    34381 gacagagaaa gcaaaaataa atgcttgact ctgtagcggg aaagcgtatt atgaacaccc
    34441 tgcgc
//
LOCUS       DAJTQY010000034        34075 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.034, whole genome shotgun sequence.
ACCESSION   DAJTQY010000034 DAJTQY010000000
VERSION     DAJTQY010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34075
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.034"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            <1..578
                     /locus_tag="OO349_001739"
     CDS             <1..578
                     /locus_tag="OO349_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5866848.1"
                     /translation="ERNAFYEGVEFPDDCDVLISYAPPKDESLVASFITQYAVTAYAS
                     QRYLEKHPISRPDELEHHSCILIDSMMIDDANIWRFNVAGSKEVRDYRVKGNYVCDNT
                     QSALELARNHLGIVFAPDKSVQSDLQDGTLVPCFQHPYEWWLDLVAIFRKREYLPWRV
                     QYVLDEMLREIRHQLAQSQQLRPEQAAESED"
     gene            complement(575..1711)
                     /locus_tag="OO349_001740"
     CDS             complement(575..1711)
                     /locus_tag="OO349_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR5866849.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTISTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQAR
                     VDRLMADIVTAEHKQRALGAELDEMAANTQQAKATRDKFAKEYQRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSSAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNALPGKVFSGKLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELDEHT
                     DLSALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWVHYLYLDH"
     gene            complement(1717..2040)
                     /locus_tag="OO349_001741"
     CDS             complement(1717..2040)
                     /locus_tag="OO349_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HCR5866850.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLIAKKRNHPHADAI
                     HVAGWVSLFTLHVIWPFLWIWATLYRPERGWGMQIHDSSVVQLQQRIAGLEKQLADIK
                     SSSAE"
     gene            2223..2366
                     /locus_tag="OO349_001742"
     CDS             2223..2366
                     /locus_tag="OO349_001742"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5866851.1"
                     /translation="MNNSSKTSTVQIKRIKPSIIYRLLLIGLGSPMVIYGLVRPLTIE
                     TRD"
     gene            complement(2585..4123)
                     /gene="aldB"
                     /locus_tag="OO349_001743"
     CDS             complement(2585..4123)
                     /gene="aldB"
                     /locus_tag="OO349_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HCR5866852.1"
                     /translation="MTNNPPSAQIKPGEYGFPIKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(4288..5439)
                     /gene="yiaY"
                     /locus_tag="OO349_001744"
     CDS             complement(4288..5439)
                     /gene="yiaY"
                     /locus_tag="OO349_001744"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HCR5866853.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDNMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHNK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVVIYRAAM"
     gene            complement(5629..7473)
                     /gene="selB"
                     /locus_tag="OO349_001745"
     CDS             complement(5629..7473)
                     /gene="selB"
                     /locus_tag="OO349_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HCR5866854.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETANAGQRIALDIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHA
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQNDSEALAVHLQRGAVNLPDFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
     gene            complement(7470..8861)
                     /gene="selA"
                     /locus_tag="OO349_001746"
     CDS             complement(7470..8861)
                     /gene="selA"
                     /locus_tag="OO349_001746"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HCR5866855.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLVAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            complement(8959..9567)
                     /locus_tag="OO349_001747"
     CDS             complement(8959..9567)
                     /locus_tag="OO349_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HCR5866856.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            complement(9638..10723)
                     /locus_tag="OO349_001748"
     CDS             complement(9638..10723)
                     /locus_tag="OO349_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR5866857.1"
                     /translation="MDLLIVLTYVALATAALGGVFLVSGLILLMNYNHPYTFTAQKAV
                     IAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQARVDRLQADLMTATHNIKT
                     LRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNSFSERDIDDARQNFLAQDAL
                     VKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLEQTVIRAPSNGYVTQVLI
                     RPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGDDAEVVFNALPGQVFHGK
                     LTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPNDDIDALPDGIYAQVAVYS
                     DHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(10726..11088)
                     /locus_tag="OO349_001749"
     CDS             complement(10726..11088)
                     /locus_tag="OO349_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HCR5866858.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATEPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            11625..13526
                     /gene="mtlA"
                     /locus_tag="OO349_001750"
     CDS             11625..13526
                     /gene="mtlA"
                     /locus_tag="OO349_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HCR5866859.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGKRGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSFDDSSANLFKLGAENIFLGRKAATKEE
                     AIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKTGVVF
                     CQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTTSVDE
                     VLELLAGRK"
     gene            13621..14769
                     /gene="mtlD"
                     /locus_tag="OO349_001751"
     CDS             13621..14769
                     /gene="mtlD"
                     /locus_tag="OO349_001751"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCR5866860.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            14769..15356
                     /gene="mtlR"
                     /locus_tag="OO349_001752"
     CDS             14769..15356
                     /gene="mtlR"
                     /locus_tag="OO349_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HCR5866861.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAVENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(15368..15577)
                     /locus_tag="OO349_001753"
     CDS             complement(15368..15577)
                     /locus_tag="OO349_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5866862.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            15861..16223
                     /locus_tag="OO349_001754"
     CDS             15861..16223
                     /locus_tag="OO349_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HCR5866863.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLEEMTGFSKTTF"
     gene            16767..17450
                     /locus_tag="OO349_001755"
     CDS             16767..17450
                     /locus_tag="OO349_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HCR5866864.1"
                     /translation="MNLKKIFFSAVTVSVLCALTGCDYIEEGKPESSLLKQQEEHNNK
                     IVLLEKQQAQLKSQLETIQKQQTGIINSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKY
                     FILNNGSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNATWGGEKPVDAKEF
                     ARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSA
                     ENRQKRSKK"
     gene            17494..21859
                     /locus_tag="OO349_001756"
                     /pseudo
     CDS             17494..21859
                     /locus_tag="OO349_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033191.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
     gene            22227..23882
                     /gene="lldP"
                     /locus_tag="OO349_001757"
     CDS             22227..23882
                     /gene="lldP"
                     /locus_tag="OO349_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HCR5866865.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLNKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            23882..24658
                     /gene="lldR"
                     /locus_tag="OO349_001758"
     CDS             23882..24658
                     /gene="lldR"
                     /locus_tag="OO349_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HCR5866866.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            24655..25845
                     /gene="lldD"
                     /locus_tag="OO349_001759"
     CDS             24655..25845
                     /gene="lldD"
                     /locus_tag="OO349_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HCR5866867.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTILLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            26041..26514
                     /gene="trmL"
                     /locus_tag="OO349_001760"
     CDS             26041..26514
                     /gene="trmL"
                     /locus_tag="OO349_001760"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HCR5866868.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(26567..27388)
                     /gene="cysE"
                     /locus_tag="OO349_001761"
     CDS             complement(26567..27388)
                     /gene="cysE"
                     /locus_tag="OO349_001761"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HCR5866869.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPVRIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(27468..28487)
                     /gene="gpsA"
                     /locus_tag="OO349_001762"
     CDS             complement(27468..28487)
                     /gene="gpsA"
                     /locus_tag="OO349_001762"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HCR5866870.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(28487..28954)
                     /gene="secB"
                     /locus_tag="OO349_001763"
     CDS             complement(28487..28954)
                     /gene="secB"
                     /locus_tag="OO349_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HCR5866871.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(29017..29268)
                     /gene="grxC"
                     /locus_tag="OO349_001764"
     CDS             complement(29017..29268)
                     /gene="grxC"
                     /locus_tag="OO349_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HCR5866872.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGCTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(29410..29841)
                     /locus_tag="OO349_001765"
     CDS             complement(29410..29841)
                     /locus_tag="OO349_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCR5866873.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKSVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            30086..31630
                     /gene="gpmM"
                     /locus_tag="OO349_001766"
                     /gene_synonym="pgmI"
     CDS             30086..31630
                     /gene="gpmM"
                     /locus_tag="OO349_001766"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HCR5866874.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLAGA
                     VHKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            31640..32923
                     /gene="envC"
                     /locus_tag="OO349_001767"
     CDS             31640..32923
                     /gene="envC"
                     /locus_tag="OO349_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HCR5866875.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVTMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRERQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            32927..33886
                     /locus_tag="OO349_001768"
     CDS             32927..33886
                     /locus_tag="OO349_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HCR5866876.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(33873..34075)
                     /locus_tag="OO349_001769"
                     /pseudo
     CDS             complement(33873..34075)
                     /locus_tag="OO349_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
BASE COUNT         8464 a         8928 c         8688 g         7995 t
ORIGIN      
        1 ttgagcgtaa tgccttttat gaaggcgtcg aatttcctga tgattgtgat gttttaatca
       61 gctacgctcc accaaaagat gaatcgctgg ttgccagttt tattacacaa tatgccgtaa
      121 ccgcttatgc cagccagcgc tatcttgaga aacatcctat tagccgtcct gacgaactcg
      181 aacatcactc ctgtattttg atcgactcga tgatgatcga cgacgcgaat atctggcgct
      241 tcaacgtcgc cgggagtaaa gaggtgcggg attatcgcgt gaagggaaat tacgtttgcg
      301 ataacacgca atcggcgctg gagctggcac gaaatcacct ggggattgtg tttgcgccag
      361 acaaaagtgt gcagagcgat ctgcaagacg gcacgctggt gccctgcttt cagcacccgt
      421 atgaatggtg gctggatctg gtggctatct tccgtaaacg ggaatacctg ccctggcggg
      481 tccagtatgt gctggatgaa atgctgcgtg aaattcgcca ccagcttgct cagtcgcagc
      541 aactgcggcc tgaacaagcg gcggaaagcg aagattaatg atcgagataa aggtaatgca
      601 cccagctggt catgcgtaat agcactttgc gcatcaccga gacatggctg aaatgatcag
      661 agtagaccgc cacctgggcg taaataccgt ctggtaaagc gctcaaatca gtgtgctcat
      721 ccagttcaat ggtcgcgata acgccatctg aacccggcgc tgtgtttaac gtctgtaagg
      781 tgccggtcga ctgataagct ccgccgggaa cagcaggact aatggctgcc agcttgccgc
      841 tgaatacctt acctggcaga gcattaaaca ccacttccgc atcgtcgcca ggggccagtc
      901 gcagcaagga gttctgacgg aactgcgcca caatttgtcg tttctgatcg ggtataaaca
      961 ccatcaccgg acgtagcggc agcgacgcgg cataggtacc cggacgaatc agcacctggg
     1021 tcacataacc atcgctcggc gcacgcacta tcgtctgctc aaggttatat tttgcttccg
     1081 cgagctgcgc tttcaggctg gcgatttgag aatgttcacc caacaccagg ctatccagct
     1141 ggctctggat ctgtttttgt tccgccgccg atgactttac ggaggcttcc tgcgccagat
     1201 aattttgccg cgccacatca atatcgcgtt ctgaaaacgg gtttactttc gcctggctgc
     1261 cgcgtgcgta acgctgatac tctttagcga atttatcccg cgtggccttt gcctgctgag
     1321 tattcgccgc catctcatct aactctgcgc ccaacgcccg ctgtttatgt tctgcggtaa
     1381 cgatatccgc catcagccga tccacccgcg cctgataacg cgtcgggtcc agtcgaaata
     1441 gcacctcacc ttttttaatc agcgtatttt tcttatccgt cacttcgatc accacgcctg
     1501 tcacctgtgg gacaacagga atagaaataa ccgctttttg cgccttgaag gtatacggat
     1561 ggttatagtt cattaacaga attaaaccac tgacaataaa tatgccaccc agggccgctg
     1621 tggaaatggt ccatttattt acaggaattt tgaagatctt aaacattgcc catgcaaaag
     1681 ccacataggt caaaataatc agtaaatcca taagtattac tcggcagaag aggatttgat
     1741 gtcggcgagc tgtttctcca gcccggcaat acgctgttgt agttgcacaa cggaggagtc
     1801 atggatttgc attccccatc cccgctccgg gcggtaaagc gtggcccaaa tccacaaaaa
     1861 tggccagata acatgcagcg taaaaagact cacccaacca gcaacatgaa tggcgtcggc
     1921 atgaggatga ttacgttttt tagcaatcag gtagggaata tcatgtaaaa taataatccc
     1981 atagaatatc accagaaata caaaaataag cactcccagt gcaaaatagt ccaggaacat
     2041 atttccctca aagaatataa aaaagaacaa ttaacgcata ttatgaaatg ccatgatgca
     2101 gtgcaatttc cttatttata taacgacata ttgtttttta tatactttct gaaatgtgct
     2161 gtctggtttt tcaacaggac gaggtttatc atattgaaat agtggaacta ttggccaaac
     2221 taatgaataa ctccagtaaa acatccacag tacagattaa gcgtattaaa ccttcaatta
     2281 tctaccgttt attgctgatt ggcctcggat caccaatggt gatttacggc ctggttcgcc
     2341 cgctcaccat cgaaacgcga gattaaactc ctgacaaaac acgccccaga aacgcacttc
     2401 tgttggcgta gtctgggtta ttgcgcacgt aggtttaaaa cgtaaattgt aacaacgtgc
     2461 gctttgttta tgccggatgc ggcgtgtacg ccttatccgg cctacggtca ggttcccgta
     2521 ggcatgataa gacgcgtaag cgtcgcatca ggcaatgaat acccaatgcg accagcttct
     2581 tatatcagaa cagccccaac ggtttatccg agtagctcac cagcaggcac ttggtttgct
     2641 ggtaatgctc cagcatcatc ttgtgggttt cgcgaccgat acctgattgt ttgtagccac
     2701 caaacgcagc atgtgccggg taagcgtgat aacagttggt ccacacgcgc ccagcctgta
     2761 tgccgcgccc catcttatag gccagattac cgttgcggct ccagacgccc gcgcccaggc
     2821 catattgcgt atcgttcgcc agctccagcg cttcttccat cgttttgaag gtggtcaccg
     2881 ccagcaccgg gccaaaaatc tcctcctgga acacccgcat attgttctga ccaaacagaa
     2941 tcgtcggttc gaggtagtag ccgtctttca gttcaccttc cagcagcttg cgccgcccgc
     3001 ctgtgagcac gtcagcgccc tcttttttac cgatatcaat gtagttgagg atggtttcca
     3061 gttgcccgtg agaaacctgc gcgcccattt gcgtcacgct gtcgagcggg ttaccgctgc
     3121 gaatgctttc gacacggcgg atggcgcgtt ccataaagcg ttcgtagatt gactcctgca
     3181 ctaaggcacg actcggacag gtgcaaactt cgccctggtt aaaggcaaac agtgcaaagc
     3241 cttccagcgc tttatcgaaa aaggcatctt cttcatccat cacatcagca aagaagatat
     3301 ttggcgactt accgcccaac tccagcgtca ccggaataat gttttgcgtt gcgtattgca
     3361 taatttgttg gcccacttcc gttgagccgg taaacgccac tttggcgatg cgtttcgagg
     3421 tcgccagata ttcgccaatt tccccacctg cgccattgac cacgttcacc acgcccggcg
     3481 gcagtaaatc accgacaatt tccattagca gcagtacaga aagcggggta agacgtgcgg
     3541 gtttcagcac cacacagttg cccgccgcca gcgcgggagc cattttccag ctcgccatca
     3601 gcagcgggaa gttccacggg ataatctgcc ccaccacgcc taacggttca tggaaatgat
     3661 aggccacggt ttcgctatca acttcactga tcccaccttc ctgcgcccga atacacgagg
     3721 cgaaatagcg gaaatggtca atcgccagcg gtacatccgc agcactggtt tcgcgaatgg
     3781 gtttgccgtt atcccaggtt tcagctgtcg ctaacagctc gaggttttgt tccattcgat
     3841 cggcaatctt aaacagaatc gccgcgcgat cctgcaccga ggtgtgcgcc catttatctt
     3901 tcactttgtg cgcagcatcc agcgccagat cgatgtctcg tttgcccgaa gacgccactt
     3961 cgcacagcag ctgcccggtc accggcgtca gattctggta atactcacca tcggcagggg
     4021 ctacccattc accgccaata aagttgtcgt agcgggcttt taacttgatg gggaaaccat
     4081 actcgccggg cttaatctgt gctgaagggg gattattggt catgatcgtc tccttgcggt
     4141 gtgaggtata acaagggtag acgtgactgg cgaaatcttc gccagtcggt aacagcttta
     4201 cgacagctat cacgaattta cgggcaagga ttacagagat agttgaaaaa gcggctgaca
     4261 atttgccagc cgttgttgga atgatgatta cattgcggcg cgataaatca ccacaatttc
     4321 ttcgtgagtt gcctggatcg ggttagtaaa gccacaggca tctttcaggg cattagtcgc
     4381 cagaaccgcg aaatcttctt ctttcacgtt caggtcgcgt aggcctgccg ggatatccac
     4441 tttcttcgcc agttcacgga tggcgttaat gcaggcttca gcaccttccg cgtcgttttt
     4501 acctgtcacg ttcacgccca ttgcagcggc acagtcacgc agacgtgcag cggcgacttt
     4561 gctgttgaat acctgaacat gcggcagcag aacggcgtta catacaccgt gtggcaggtt
     4621 gtagaaaccg cccagctggt gcgccatcgc atgaacataa cccagagaag cattattgaa
     4681 cgccataccg gcaaggaact gggcataagc cattgcttca cgcgctttcg cattactgcc
     4741 atcttcaacg gctaacggca ggttttcggc aatcatggtc acggctttca gtgcacaagc
     4801 gtcagtgatc ggcgtggcgg caatagaaac atacgcttcg atagcgtgcg ttaaggcatc
     4861 catacccgtt gcggcggtca gtgacttcgg cataccaatc atcagagagg agtcattgac
     4921 agaaagcagc ggagtcacgt gcttatcaac aatcgccatt ttgttatgac gcgcttcgtc
     4981 agtgatgatg cagaaacggg tcatttcaga ggccgtaccc gccgtggtat tgatggcgat
     5041 catcggcagc tgcggttttg cagagcggtc aacgccttcg taatcgcgaa tatcgccgcc
     5101 attggctgcc accagcgcaa taccttttgc gcagtcgtgt ggagaaccac cgcctaagga
     5161 gatcacgcta tcgcaattat tttctttaag taatttcaaa cctgcggcga cgttttccgt
     5221 ggtggggtta ggttgggtgc catcataaat aacgctaaaa atattgcgtt cttccagtgc
     5281 tttttgcaca tcacccgcca tacctaattt cgttaacata ttgtcagtga caattaaggt
     5341 acgggtaaat ccataatctg ccatcatatt cattgcatca gtcaatgaat cagcgccgat
     5401 gacattcaca gaaggaataa agaaagttga agatgccata acacactcct gaaagtgtaa
     5461 aaaagggtgg gcagcttact gaggattttc attcgaaaac atgatcatta ttattaatta
     5521 tcaaaattaa tctaataaaa agcacattgt attcattaaa caatgtgctt tatttaaaat
     5581 cataagaaat ttgtattaat ttattttaaa aattaattaa tcatttcctt atttttccgg
     5641 aaataataat gcgtcgcgca ataaatgatc atttccacga cgacgcgtaa agccaatgcg
     5701 gtcaaaatat tccagaatct gaattgccag ctttcggcct acgcctaagc gatcgcggaa
     5761 atccgccgcg caggttgaac cacactcctg atcgagatcg cggatcatat tggcaaattc
     5821 gacaatccga tcgttacggt aataacgatc tttgacgatc gcggtaatta ttccttgctg
     5881 cgccgcctgg cgtagcgtca ggcgcattgc ctgctcgtcg gtacccgtct cttttgccag
     5941 atcacgcacc caccacggct cgtcaccaaa cagcggctct gctttttgcc agatggcctg
     6001 ctgctcttcg ctgaagcccg ctttgtgatc cggcagatgc agccaaccgt gatgactgag
     6061 gatgtcgccg ctttcgcgca tcttttcaat cagcaacagt actagcgctt catcttccat
     6121 tggcaacgcc atacgtcgca gacgttcgcg cccaggccca ggttcatcgc gatgttgctc
     6181 atgataagtc gctaatgtgt cgagaatttt ccgttgccag cgagcggcaa ccggcgcatt
     6241 caacaagcta taaccagcct gaatatagcc aggctgttgc agcagttcgc gcatcccttc
     6301 gccgttgagc tggcgcgccc aggcgaaatc aggaagatta accgcgccgc gttgcaggtg
     6361 aacggctaac gcttcgctat cattctgtgc cctggcgagg gaagccagcc attgcagata
     6421 ctctggttta cgtttaccgc gacgcggcgg gttaagcatc acgacgcgcg ctccggccag
     6481 cgtgttgcgg gcagagatat cgcgcaatac caggcggtcg ttatctgcca gccataacgg
     6541 ggtgtcgaag accagttcag caaggttatc ttccagcagt gaaacgcgtc ccgtgacgtg
     6601 gctggcggcg tggtgaatat gcagcggctg ccactgggtc agcggtgcat gggtttgaag
     6661 ctcgacaatc acccgtgtga acggctctgg cggcacatcg gcaagcagcc agtcgccacg
     6721 gttaatctgc tctttttccg catcacccgc gatgtcaagc gcgatacgct gcccggcatt
     6781 ggcggtttct gttggctggt tttgcgcatg cagcgcacgt acacgcatcg gtttatttac
     6841 accagtcagc cagagtgaat cgcctacctt cacttccccg cttaacgccg taccggtgac
     6901 gaccagcccg gcacctttta cggtaaatgc gcggtcaatc gcgaggcgga aactatgttg
     6961 gctggcgtgc tcgcgttccg gcaactgaag cagatgctcg cgcagggcat caattccccg
     7021 accttcggtt gctgcggtga taaacagttt tgcctcagca aaaccgtatt cccgcagaac
     7081 ctcctttacc tggcgttcaa cctcatcaac acgcgcttcg tccacgcgat cggctttggt
     7141 cagcgccact gtcagcatcg ggttaccggt cagctgcaaa atcgccagat gctcacgggt
     7201 ctgtgccatc acgccgtcat cgcacgccac caccaacagc gcgtgatcga taccaccaac
     7261 gcccgccagc atgttggaaa gaaacttttc atgaccggga acgtcgataa aaccaggcac
     7321 gcgaccatcc ggctgcggcc agtaggcata gccgagatcg atggtcatgc cgcgcttttt
     7381 ttcttccggc agacggtcag catttacgcc agtaatcgcc tgcaataagg ttgttttgcc
     7441 gtggtcaacg tgtccggcag tcgcaataat catttcaaca acatctccaa aaaccgttgc
     7501 tcatcttcaa ggcagcgtaa atccagccac aatcgtccgt cataaatacg accaatcacc
     7561 ggcactggca attcacgcca gcgggcggct aatgactcaa ggtggctacc gcgtccatca
     7621 tggggtgtaa acgttaatgc cgcgctcggc aggcgatcaa ccggcagcga accactgcca
     7681 atctgcgaaa gacatggcat aacctgtacc gcaaactccg cgccgtaatg tgcgacaagg
     7741 ggagcctgta aacgttgtgc ctggatttga atgacctctg cgctgcgggt aagcaggcgc
     7801 agggtcggta atttttcact cagagcttca gggtgtaaat aaagacgcaa cgtggcttcc
     7861 agcgccgcga gggtcatttt atccgcgcgt aatgcacgct tcagcgggtg gctttgcagg
     7921 cgggcgatca tctctttttt accaacgata atcccggcct gcggcccgcc taacaacttg
     7981 tcgccggaga agctcaccag actgacgccc gccgcaatca actcctgcgg cattggctct
     8041 ttcggcaaac cgtactggct aagatcgacc agcgagccac tgcctaaatc agtcaccact
     8101 ggaacatcca gctctttgcc gagcgccacc agttcagctt cgtctatcgc tttggtgaac
     8161 ccctgaatgc tgtagttact ggtatgtact ttcatcaaca gtgcggtatt ttcattcacc
     8221 gcctgacgat aatcattcgc gtgcgtgcgg ttggtggtcc ctacttcgtg tagggtgcag
     8281 cctgcctgac gcataacatc gggaatacga aacgcgccgc caatctccac cagttcgccg
     8341 cgagatacca ccacctcttt cccgctggca gtggccgcca acatcaataa caccgccgcc
     8401 gcattgttat tgacgataca ggcatcttcc gcccccgtaa tacggcacag cagctgcgcc
     8461 agcgcccgat cgcgatgtcc gcgtccggcg tcgtccagat cgtactcgag ggtcactggc
     8521 gaacgcatag cctgcgcaac ggcttccacc gcggcttccg cctgtaaagc tcgcccaagg
     8581 ttggtatgca gcacggttcc cgtcaggttg atcaccggac gcagcgcgct ctgcgcctct
     8641 ttcgtcaacc gggcatcgac ttcttgcgcc cagttttcac accacgcagg cagcgtctgg
     8701 ctgccacgaa tcacttctcg cgcttcgtcg agcatctgac gcaacaattc caccacgcgg
     8761 gtgtgaccat aagtatcacg caaagaaagg aaggagctat cgcgcaataa gcgatcaata
     8821 gccggaagtt gactatagag ggaacgcgtt tcggttgtca taggaaacct ggctgatcaa
     8881 ggccctctca cacggagaag ggcgtttaac ataaccacgg attgtaacgt gagatgggtc
     8941 aggaggacat atcgcgcatc aagcctttgg cggttcggtg cgagcaaaac tttcgcggct
     9001 aaacaggttt tcgaccagtt tgactaagtg tgggcggtcg acacaccaac ctggcgcaac
     9061 gcggcggaaa ttgagatatc cgacagcaca ggcaatggcg atagtcgcca gattgaccgt
     9121 atcggttttg agtgtgccat cgaccagata tccttccagc acatccagac tgcggttgat
     9181 tttctcccgc tggcgtaaca attcatcttc agactgctgc gccgccggac gcgcctgttc
     9241 acgcaccgat accagcccgg catccataat gccatccgcc agcgcctcaa ttttgcgcac
     9301 ccgcaacgac tccagcggat cgcgcggcaa catcgccgga gcgacattca ttaactcaat
     9361 atattcagcg atgatcggcg aatcaaacca gcattcgccc tcttcggtca ccagcaccgg
     9421 cactttccct aacgggttaa attgcgccac gccgttatcc gcgttatagg gcagttcatt
     9481 aataaattcg aaagttatgc ccttttctaa caacagaata gaaagtttgc gtacaaacgg
     9541 gctggtgtag ctaccgacga gtttcatgcc gagtcctttg tgcgaggaaa aatatcagta
     9601 tggctcaggc tcacgcaaaa cacaggtgat ataagtctca atgatccaaa taaagataat
     9661 gcatccaact ggtcattctt agcagcactt tccgcatcac cgaaacatgg ctgaaatggt
     9721 cggagtaaac cgccacctgg gcgtagatgc cgtcgggtaa ggcatcgata tcatcgttag
     9781 ggtccagttc aatggttccc agcacaccgt ccgtgccggg aacgaccgtt aatgattgca
     9841 ataccccctg cgcctgataa gaaccgcctg gcacgacagg taaaatacta gtcaatttgc
     9901 cgtggaacac ctgcccaggt agcgcgttaa acaccacttc cgcatcatcg ccaggtttca
     9961 gacgtaacag cgagttttgc cgaaattggg cgacaatttg ccgtttttgc tcggggatga
    10021 agaccatcac cggacgcagc ggcaaggcag ctgcgtatgt acctgggcgg atcagtacct
    10081 gagtgacgta gccattgctt ggtgcgcgaa tgacagtctg ctcaagatta tattttgctt
    10141 cagtaagttg cgctcttaag ctcacaatct gcgattgctc gccgttgacc atactgtcga
    10201 gctgactctg gatctgcgcc tgctccgcca ccgagccttt cactaacgca tcctgcgcga
    10261 ggaaattttg ccgcgcatcg tcgatgtcac gttccgagaa cgaattcacc gccgcctggc
    10321 tgcctttcag gtaacgttga taatttttaa acagacggtc gcgctccgct gaaacctggg
    10381 tggtgttggc ctgcgcttca gtgagctgcg cacgcagcgt ctttatatta tgcgtcgccg
    10441 tcatcaggtc agcctgaagt ctgtcaactc gcgcctggta acgaaccggg tcgagcttaa
    10501 aaagcacctc gcccttttga ataagctgat tattcttgtc agtgacttca gtaacaattc
    10561 ccgtcacctg tggcgtgata gggatcgcta tcactgcctt ttgcgcggta aaagtgtaag
    10621 ggtggttgta gttcatcaac aaaatcaaac cactcaccag aaacacgcct cccagcgccg
    10681 ccgtcgccag cgccacgtaa gttaaaacaa tcaatagatc cataattaga tctccgggaa
    10741 cgtggaatag tcagtctttt tctcggcggc gagttgatgc tccagccggg aaattcggtc
    10801 agaaagtgcg gcgatttccg gttcagtcgc tttctcctgc gacgcaacat gtgactgcat
    10861 accccagcca cgctccggtt gatagagcgt cgcccagatc cacagaaacg gccagataac
    10921 atgcagagta aacaggctca cccagcccgc cgtatgaata gcgtcggcat gggggtgatt
    10981 gcgctttttg gcaatcagat aagggatgtc atgtatcgcg atgattccat aaaaaatcac
    11041 cagaaagacg aagatcagca ctcccaacgc gaaatagttt agaaacatat ctgcctcgga
    11101 ttcacgttta tcagtgttgt tttttggctg gcagccagaa gggagtcagg ctgatatttt
    11161 gacaataatc cgggttcgcg attctcgcca taacaccaaa gaataatttt tagaggtgat
    11221 gagttgctta gttacataac gattgtatga cgaaggcata acatgctgta gatcacatca
    11281 ggtgaacgcc gtaagaaaat atcttgtgat tcagatcaca aagattcaac aaaccatcaa
    11341 aacaaaaatg tgacattact cacatttaaa tgccattttt agcgaaaatc accgccttgt
    11401 ttctttttta tacaggcgtt ttgtgatgag cgtcacgtca attacctctc taccccctat
    11461 atttatgtga ctgatatcac acaaaagccc gtcgactgga cagttaaccg attcagtgcc
    11521 agatttcgca gtatctacaa ggtccggcta cctctgccgc cacattaaca aaaaacctcg
    11581 ggcttccagc ctgcgcgaca gcaaacataa gaaggggtgt ttttatgtca tccgatatta
    11641 agatcaaagt gcaaagcttt ggtcgtttcc tcagcaacat ggtgatgcca aatatcggcg
    11701 cgtttatcgc gtggggtatc atcaccgcgt tatttattcc aacagggtgg ttaccgaacg
    11761 agacgctggc gaagctggtc gggccgatga tcacttatct cctaccgctg ctgatcggtt
    11821 ataccggtgg taagctggta ggcggcaaac gtggtggcgt agtcggtgcc attaccacca
    11881 tgggcgttat cgtcggcgca gacatgccga tgttcctcgg ttctatgatt gcaggtccgc
    11941 tgggcggctg gtgcattaag cacttcgacc gctgggtaga cggtaagatc aaatccggtt
    12001 ttgagatgct ggtgaataac ttctccgcag gcatcatcgg gatgatcctc gctattctgg
    12061 cattcctcgg cattggcccg attgttgaag ccctgtccaa aatgctggct gcgggcgtta
    12121 acttcatggt tgtccatgac atgctgccgc tggcgtctat ctttgttgaa ccggcgaaaa
    12181 tcctgttcct caacaacgcc attaaccacg gtatcttctc gccgctgggt attcagcagt
    12241 cccatgaact gggtaaatca atcttcttcc tgattgaagc taacccaggt ccaggtatgg
    12301 gcgtgctgct ggcgtacatg ttctttggtc gtggtagcgc taaacagtct gcgggcggtg
    12361 cggcaatcat ccacttcctg ggtggtatcc acgaaatcta cttcccgtat gtgctgatga
    12421 atccgcgtct gatcctcgca gtcatcctcg gcggtatgac tggcgtgttc acgctgacta
    12481 tcctgggcgg tggtctggtt tctccggcat ctccgggttc tatccttgct gtactggcga
    12541 tgacaccaaa aggtgcttat ttcgctaaca tcgcgggtgt gtgtgcggcg atggctgtct
    12601 ccttcgttgt ctctgctatt ttgctgaaaa ccagcaaagt gaaagaagaa gatgatattg
    12661 aagcagcaac tcgtcgtatg caggacatga aagctgagtc taaaggcaca tctccgctat
    12721 ctgcgggcga tgtgactaac gacctgagcc acgtacgtaa aatcatcgtt gcctgtgacg
    12781 ccggtatggg ttcaagtgcg atgggcgcag gcgtgctgcg taagaaaatt caggatgcag
    12841 gtctgtcgca gatttcggtc actaacagcg cgatcaacaa cctgccgcca gatgtggacc
    12901 tcgtcatcac tcaccgtgac ctgaccgaac gcgctatgcg ccaggttccg caggcacagc
    12961 atatttcgct gaccaacttc ctcgacagcg gcctgtacac cagcctaacc gaacgtctcg
    13021 ttgctgccca gcgccatact gaaaacgaag tgaaagtgaa agacagcttt gacgattcca
    13081 gtgctaacct gttcaaactg ggcgcggaga acatcttcct cggtcgcaaa gcagcaacca
    13141 aagaagaagc gattcgtttt gctggcgagc agctggtgaa aggcggttac gttgagccgg
    13201 aatacgttca ggcgatgctg gatcgtgaaa aactgacccc gacttatctg ggtgagtcta
    13261 tcgcggtgcc acacggtacg gttgaagcga aagatcgcgt actgaaaacg ggcgtggtgt
    13321 tctgccagta cccggaaggc gtgcgcttcg gtgaagaaga agatgacatt gcccgtctgg
    13381 tgattggtat tgcagcccgt aacaacgagc acattcaggt tatcaccagc ctgaccaatg
    13441 cactggatga tgagtccgtc atcgagcgtc tggcacacac caccagcgtg gatgaagtgc
    13501 tggaactgct ggcaggtcgt aagtaatcca atcctaccct ctcctcacgg agagggtttg
    13561 ggtgagggaa aagcctcacc ccagccctct cgggtaaaaa cattgatgaa ggttaatact
    13621 atgaaagcat tacattttgg cgcaggtaat atcggtcgtg gctttatcgg taaactgctg
    13681 gcagacgcgg gtatccaact gacgtttgcc gatgtcaatc aggtggtact tgatgccctg
    13741 aatgcccgtc atagctatca ggttcatgtg gtcggtgaaa ccgagcaggt agataccgtt
    13801 tccggcgtca atgctgtcag cagcattggt gatgatgtcg ttgatctgat tgctcaggtc
    13861 gatttagtca ctaccgccgt tggcccggtt gtgctggaac gtattgctcc ggctatcgcc
    13921 aaagggctgg tgaaacgcaa agaacaaggt aatgaatccc cgctgaacat catcgcctgt
    13981 gaaaacatgg tacgcggcac cacgcagctg aaaggtcatg tgatgaacgc cctgccggaa
    14041 gacgccaaag cgtgggtaga agagcacgtt ggctttgtcg attccgccgt tgaccgcatc
    14101 gtaccgcctt cggcttcggc aactaacgat ccgctggaag tgacggtaga aactttcagc
    14161 gaatggattg tcgataaaac gcagttcaaa ggcgcactgc cgaacatccc aggcatggag
    14221 ttaaccgaca acctgatggc atttgtcgaa cgtaaactct tcaccctgaa cacgggtcat
    14281 gctataaccg cgtacctcgg aaaactggcc ggtcatcaga ccattcgtga cgctattctc
    14341 gacgagaaaa tccgcgcggt ggtaaaaggt gcgatggaag aaagtggtgc ggtactgatc
    14401 aagcgctacg gctttgacgc agacaaacat gcggcgtaca tccagaaaat cctcggtcgt
    14461 tttgagaacc cgtatctgaa agatgatgta gagcgcgtag gccgtcagcc gctgcgtaaa
    14521 ctgagtgctg gcgaccgtct gatcaagcca ctgctcggta cgctggaata cagccttccg
    14581 cacaagaatc tgattcaggg gattgctggt gcaatgcact tccgcagtga agatgatccg
    14641 caggctcagg aactggcagc actgatcgct gacaaaggtc cgcaggcggc gctggcacag
    14701 atttccggtc ttgatgccaa cagcgaggtt gtatccgagg cggtaaccgc ttataaagca
    14761 atgcaataat ggtggaccag gcgcaggaca ccctgcgccc gaataacaga ttgtcagata
    14821 tgcaggcaac aatggaacaa acccaggccg ttgaaaaccg tgtgcttgag cgtctgaatg
    14881 ctggcaaaac cgtgcgaagc tttctgatca ccgccgtcga gcttttgacc gaggcggtaa
    14941 atcttctggt gcttcaggta ttccgcaaag acgattacgc ggtgaagtat gctgtagaac
    15001 cgttactcga cggcgacggt ccgctgggcg atctttctgt gcgtttaaaa ctcatttacg
    15061 ggttgggcgt cattaaccgc caggaatacg aagatgcgga actgctgatg gcattgcgtg
    15121 aagagctaaa tcacgacggc aacgagtacg cctttaccga cgacgaaatc cttggaccct
    15181 ttggtgaact gcactgcgtg gcggcgttac caccgccgcc acagtttgaa ccagcagact
    15241 ccagtttgta tgcaatgcaa attcagcgct atcaacaggc tgtgcgatca acaatggtcc
    15301 tttcactgac tgagctgatt tccaaaatca gcttaaaaaa agcctttcaa aagtaagcaa
    15361 cgtctgctta ctgcccctct acctgcttcg gccgataaag ccgacgataa tactccagac
    15421 gttgtagata taacggcacg ctttcatcgg gtattccgga cggaatcgcg ttacggggag
    15481 gaagtttttt cagatactcc cggaacgcct ggcttgatgc catgaaatct acggctttat
    15541 cgataagaag cggaacgttt tcacctaatt tgcccatgat gatatctccg tattaccccg
    15601 cgccgggaat gcgcggcctc caattttagt ttaggttccc ggtaaagatg acattaagga
    15661 aaacgtgctg aatcctcaat aatcatccgc tgctgtttat tatctttttc tatagatttc
    15721 ttaattaatc aacgaattgc gtagtttttg agcatgttaa tgattacgtt gcagaactat
    15781 tacaatgtca tcagttgtga acaaagcacc tggtcgcgca tactaggggc tataaattta
    15841 tctttatcag aagccatcac atgaaagaag tcgaaaaaaa cgaaatcaag cgtctcagcg
    15901 atcgcctgga cgccatccgc caccagcagg ccgatctgtc gctggttgaa gccgcagaca
    15961 aatatgccga gctggaaaaa gagaaagcca cgctggaagc agaaattgct cgcctgcgtg
    16021 aggttcatag ccaaaaactg agtaaagaag cacagaagct gatgaagatg ccgttccagc
    16081 gcgcaattac caaaaaagag caggctgata tgggcaagct gaagaaaagt gttcgcggac
    16141 tggtggttgt gcacccaatg accgcgctgg ggcgcgaaat gggcctggaa gagatgactg
    16201 gattttctaa gactacgttt taagcccctc agacgactgg ttcgatcgta tcaacccgca
    16261 tctggcttgc cttagctggt caatgcgggg actctcctgt ctactcgcct aattaccaaa
    16321 caattcactc cctttacttc gatttaccgc tggttgatat tctgaaaatg catttttacc
    16381 gaaatagcca tctataaaca ataaaaatat atagaagata tcatttgtta attaacaagc
    16441 atttacgtct ctttttttcg gatttaattg tgaaatttta atcatcatat tcacaaaatc
    16501 aattatctgt gaaatttttc ccagacatgg tttctttcat atttgacatt tattaataat
    16561 catcattaga atccacgaaa aataatatat tcatacaaac acagacatct tttgaaacga
    16621 atgatacatt tttatgatga atgcttaaaa caatatttaa tcgacacaca tcactgtcat
    16681 taatgtaaag caacatagct caccaataat acaatggaca actctaccgg cattgatcgc
    16741 tggtcggagg aaataaggat ttttacatga acttgaagaa gatttttttc agtgctgtca
    16801 ctgtttcagt tctctgcgca ttaacgggat gtgactacat tgaagaaggc aagccagaaa
    16861 gtagtttact gaaacaacaa gaagaacata ataataaaat tgtgctactt gaaaaacaac
    16921 aagcacagct aaagagtcaa ctcgaaacta ttcaaaaaca acaaactggc ataataaaca
    16981 gtacaaagac tttaactcac gtaattaaat ccgttaaaga ccaacaaaat acttttattt
    17041 ttaccgagtt taacccagca aaaacgaagt atttcatcct gaacaacggt tcggtggctt
    17101 tagcgggtcg agtgttatcc attgacgcca cagagaatgg tagtgttatt catatctcac
    17161 tggtcaactt attaagtaca cctatctcaa acattggttt taatgcgaca tggggtggtg
    17221 aaaaacctgt cgatgccaaa gagtttgccc ggtggcaaca attacttttc aacacatcaa
    17281 tgaaatccac attgaaatta ttaccaggtc aatggcaaga cattaatttg accctgaagg
    17341 gtgtatcgcc caataacctg ggatatctga agttagccat caacatggaa aatattcagt
    17401 tcgacaatct tccctctgct gaaaatcggc agaaaagaag caaaaaataa atgcagttag
    17461 tacgcttata acaaggaata gcgagcatca ttaatgaaca aaatatttaa agttatctgg
    17521 aaccctgcga cagggaatta tactgttacc agcgaaacgg caaaaagccg tggcaagaaa
    17581 tctgggcgca gtaagctgtt aatttctgcg ctggttgcgg gtggaatgtt gtcgtcgttt
    17641 ggggcattgg cgaatgccgg gaatgacaac ggtcagggtg ttgattacgg tagtggatca
    17701 gctggcgacg gctgggttgc tataggcaaa ggggcgaaag caaatacttt tatgaacacc
    17761 agtggttcca gtactgctgt gggttatgac gctatagctg aaggccaata tagctctgcc
    17821 atcgggtcaa aaacccatgc gattggtggt gcatcaatgg cctttggggt tagtgcaata
    17881 tcagaaggcg atagaagtat agcactgggt gcctcttcgt attcattggg ccaatactca
    17941 atggccctcg gccgttattc aaaagcattg ggtaaattgt ctattgctat gggggactct
    18001 tccaaagcgg aaggagcaaa cgccattgcc ctggggcgta gcagtgtagc tagcggtact
    18061 gacagcctcg catttggcag acaatcactt gccagcgcag cgaacgctat tgcgataggt
    18121 gctgagaccg aagccgctga aaatgcaact gctattggca ataatgcgaa ggcaaaaggg
    18181 actaatagca tggcaatggg gttcggaagc cttgccgata aagtcaatac tatcgcatta
    18241 ggaaatagca gccaggctct ggcagataat gcaatcgcca taggccaggg caacaaagct
    18301 gatggcgtgg atgccatcgc tctgggtaat ggtagccagt cgagaggctt aaacaccatt
    18361 gccttaggca cagccagtaa tgcaactggt gataagagtc ttgcgcttgg tagtaatagc
    18421 agtgccaacg gtattaactc tgtcgcgctg ggcgcagatt ccattgcgga tttagacaat
    18481 accgtctctg tcggcaatag ttcattaaaa cgcaagatcg ttaatgtgaa aaatggcgcg
    18541 atcaagtctg acagttacga tgccattaat ggttcacagc tttatgccat tagcgactcg
    18601 gtagcaaaaa aggcttggag gaggggctgc agtagatgtt gatgacggta ctgttacagc
    18661 accaacctac aatttaaaaa atggtagcaa aaataacgta ggggctgcgc tcgctgtact
    18721 tgatgaaaac accctgcaat gggaccaaac caaaggcaaa tacagcgctg ctcatggtac
    18781 tagtagccca actgccagcg taatcaccga tgttgcggat ggcacgattt cagcctccag
    18841 taaggatgcg gttaacggtt cccaactgaa agctaccaat gacgatgtcg aagccaacac
    18901 caccaatatc accaacctga cggattccgt tggtgacctt caggctgatg ccctgctctg
    18961 gaacgaaact aaaaaggcat tcagtgcagc tcacggccag gataccacca gcaaaatcac
    19021 caacgttaaa gatgccgacc tgacggctga cagcactgat gctgttaacg gctctcagct
    19081 gaaaaccacc aacgatgctg tggcgacgaa taccaccaat atcgccaata acacttccaa
    19141 tattgccact aacaccacca acatctctaa cctgactgag acggtgacta atcttggtga
    19201 ggatgcgctg aaatgggata aggacaatgg tgtattcacg gcagctcatg gcaccgagac
    19261 caccagcaaa ataaccaacg ttaaagatgg cgacctgacg actggcagca ccgatgccgt
    19321 taacggctct cagctgaaaa ccaccaacga tgccgtggcg acgaatacca ccaatatcgc
    19381 cactaacacc accaacatct ctaatctgac tgagacggtg actaatcttg gtgaggatgc
    19441 gctgaaatgg gataaggaca atggtgtctt cactgcagct catggcaaca ataccgccag
    19501 caaaatcacc aatatcctgg acggcacagt cactgcaacc agttccgatg ccattaacgg
    19561 tagccagctt tatgacttaa gcagcaatat cgccacctac ttcggcggca atgcttctgt
    19621 gaatactgac ggtgtgttta ccggtccaac ctacaaaatc ggtgaaacaa attattataa
    19681 cgtcggcgat gcactggctg cgattaactc ctcatttagc acgtctctcg gcgatgctct
    19741 gctttgggat gccaccgcag gtaaattcag tgccaaacac ggtactaatg gtgacgcaag
    19801 cgtgatcact gatgtcgcag atggtgaaat ttcagactcc agttctgacg cagtaaacgg
    19861 ctcacaactc cacggcgtga gcagttatgt tgttgatgcg ctggggggtg gtgccgaagt
    19921 caatgcagac ggcaccatca ccgcaccgac ttacaccatt gcgaacgccg agtacaacaa
    19981 cgtcggtgac gccctggatg cgcttgatga taacgccctg ctgtgggata agactgccaa
    20041 tggcggtgct ggagcctaca atgccagcca tgacggtaaa gccagcatca tcactaatgt
    20101 cgctaatggc agtattagtg aggacagtac cgatgcagtg aacggttctc agttgaatgc
    20161 gacgaatatg atgattgagc agaacaccca aattatcaat cagctcgctg gtaacaccga
    20221 cgcaacctat atcgaagaaa atggcgctgg tatcaactac gttcgtacta acgacaacga
    20281 tttagccttt aacgatgcaa gcgcctctgg tgtcggcgct acagctgtag gttataacgc
    20341 tgtcgcgtct ggtgccagca gcgtagccat tggtcagaac agcagcagca ccgttgatac
    20401 cggtattgcg ctgggtagca gctccgtttc cagccgtgtg attgccaaag gttctcgtga
    20461 cactagcgta acggaaaatg gcgtggtgat tggttatgac accactgacg gcgaactgct
    20521 aggtgcattg tcaattggtg atgacggtaa ataccgccaa atcatcaacg tagctgatgg
    20581 ttcagaagcc catgacgccg ttacggttcg ccagttgcag aacgctattg gagcggtcgc
    20641 cactacgcca accaagtact ttcacgccaa ctcaacggca gaagactcac tggccgttgg
    20701 tgaagactcg ctggcaatgg gtgcgaaaac tgtcgttaat ggtaatgcag gtattggtat
    20761 cggtttgaac actctggttc tggctgatgc gatcaacggc attgctatcg gcagtaacgc
    20821 acgcgcaaac catgcaaaca gtatcgcaat gggtaacggt tctcagacca cccgtggtgc
    20881 acagactggc tacaccgcct acaacatgga cgcaccgcag aactctgtag gtgagttttc
    20941 tgtcggtagt gaagacggtc aacgtcagat cacaaacgtc gcagctggtt cagcggatac
    21001 cgatgcggtt aacgtgggtc agttgaaagt cactgatgag cgcgtagcgc aaaataccca
    21061 gagcattact aacctgaaca atcaggtcac taatctggat actcgcgtta ctaatatcga
    21121 aaacggtatt ggcgacattg tcaccaccgg tagcaccaag tacttcaaga ccaacaccga
    21181 tggcgtagat gccaacgccc agggtaaaga tagcgttgct attggttctg gttccattgc
    21241 tgccgctgac aacagcgtcg cactgggtac cggttccgtt gcagaggaag aaaatacaat
    21301 ctctgtaggt tcttctacta accaacgccg tatcaccaac gtagctgcca gtgttaatgc
    21361 caccgatgcg gttaacgtgt cgcagctgaa atcttctgaa gcaggcggag tgcgctacga
    21421 caccaaagct gatggttcta tcgactatag caatatcacc ctcggtggtg gcaacggcag
    21481 tacgactcgt atcagcaacg tctccgctgg cgtcaacaac aacgacgcgg tgaactacgc
    21541 gcagttgaag caaagcgcgc aggaaacgaa gcaatacacc gatcagcgga tggttgagat
    21601 ggataacaaa ctgtctaaaa ctgaaagcaa gttgagcggt ggtatcgctt ctgcaatggc
    21661 aatgaccggt ctgccgcagg cttatacacc gggtgccagc atggcttcta ttggtggcgg
    21721 tacttacaac ggtgaatcgg cagttgcttt aggtgtatcg atggtgagcg ccaatggtcg
    21781 ttgggtctac aaattacaag gtagtaccaa tagccagggt gaatactccg ccgcactcgg
    21841 tgccggtatt cagtggtaat gatccattaa gttagtgtga ctaagggcaa acgccaaatt
    21901 gcctgatgcg ttacgcgcat caggcaagat aaggcgctcc gggtcaacaa tctaagggca
    21961 attctctgat gaggattgcc cttttcttta ccagacatct ccccccacaa gaattggccc
    22021 taccaattct tcgcttatct gacctctggt tcacaattcc ccaattaaaa ctcacatcaa
    22081 tattgccaat acataacatt tagttaacca ttcattgtca ttatccctac acaacacaat
    22141 tggcagtgcc acttttacac aacgtgtgac aaggagatga gcaacagact cattacacga
    22201 tgtgcgtgga ctctaggaga cctgcaatga atctctggca acaaaactac gatcccgccg
    22261 ggaatatctg gctttccagt ctgatagcat cgcttcccat cctgtttttc ttctttgcgc
    22321 tgattaagct caaactgaaa ggatacgtcg ccgcctcatg gacggtagca atcgcccttg
    22381 ccgtggcttt gctgttctat aaaatgccgg tcgctaacgc gctggcctca gtggtttatg
    22441 gcttcttcta tggtttgtgg cccatcgcgt ggatcattat tgcagcagtg ttcgtctata
    22501 agatctcggt gaaaaccggg cagtttgaca tcattcgctc gtctattctt tcgataaccc
    22561 ctgaccagcg tctgcaaatg ctgatcgtcg gtttctgttt cggcgctttc cttgaaggag
    22621 ccgcaggctt tggcgcaccg gtagcaatta ccgccgcatt gctggtcggc ctgggtttta
    22681 aaccgctgta cgccgccgga ctgtgcctga ttgttaacac cgcgccagtg gcatttggcg
    22741 cgatgggcat tccaatcctg gttgccggac aagtaacagg catcgacagc tttgagattg
    22801 gccagatggt ggggcgtcag ctaccgttta tgaccattat cgtgctgttc tggatcatgg
    22861 cgattatgga cggctggcgc ggtatcaaag agacgtggcc tgcggtcgtg gttgcaggcg
    22921 gctcgtttgc catcgctcag taccttagct ctaacttcat tgggccggag ctgccggaca
    22981 ttatctcttc gctggtatca ctgctctgcc tgacgctgtt cctcaaacgc tggcaaccag
    23041 ttcgcgtatt ccgcttcggt gacttggggg cgtcacaggt tgatatgacg ctggcccaca
    23101 ccggttacac cgcgggtcag gtgttacgtg cctggacacc gttcctgttc ctgaccgcta
    23161 ccgtaacact gtggagcatc ccgccgttta aagccctgtt cgcatcgggt ggtgcgctgt
    23221 atgagtgggt gatcaacatt ccggtgccgt acctcaataa actggttgcc cgtatgccac
    23281 cagtggtcag cgaggctaca gcctatgccg ccgtgtttaa gtttgactgg ttctctgcca
    23341 ccggcaccgc cattctgttt gctgcactgc tctcgattgt ctggctgaag atgaaaccgt
    23401 ctgacgctat cagcaccttc ggcagcacgc tgaaagaact ggctctaccc atctactcca
    23461 tcggcatggt gctggcgttc gcctttatct cgaactattc cggactgtca tcaacactgg
    23521 cactggcact ggcgcacacc ggtcatgcat tcaccttctt ctcgccgttc ctcggctggc
    23581 tgggggtatt cctgaccggg tcggatacct catctaacgc cctgttcgcc gcgctgcaag
    23641 ccaccgcagc acaacaaatt ggcgtctctg atctgttgct ggttgccgcc aataccaccg
    23701 gtggcgtcac cggtaagatg atctccccgc aatctatcgc tatcgcctgt gcggcggtag
    23761 gcctggtggg caaagagtct gatttgttcc gctttactgt caaacacagc ctgatcttca
    23821 cctgtatggt gggcgtgatc accacgcttc aggcttatgt cttaacgtgg atgattcctt
    23881 aatgattgtt ttacccagac gcctgtcaga cgaggttgcc gatcgtgtgc gggcgctgat
    23941 tgatgaaaaa aacctggaag cgggcatgaa gttgcccgct gagcgccaac tggcgatgca
    24001 actcggcgta tcacgtaatt cactacgcga ggcgctggca aaactggtga gcgaaggcgt
    24061 gctgctcagt cgacgcggcg gcgggacgtt tattcgctgg cgtcatgaca catggtcgga
    24121 gcaaaacatc gtccagccgc taaaaacgct gatggccgat gatccggatt acagtttcga
    24181 tattctggaa gcccgctacg ccattgaagc cagcaccgca tggcatgcgg caatgcgcgc
    24241 cacacctggc gacaaagaaa agattcagct ttgctttgaa gcaacgctaa gtgaagaccc
    24301 ggatatcgcc tcacaagcgg acgttcgttt tcatctggcg attgccgaag cctcacataa
    24361 catcgtgctg ctgcaaacca tgcgcggttt cttcgatgtc ctgcaatcct cagtgaagca
    24421 tagccgtcag cggatgtatc tggtgccacc ggttttttca caactgaccg aacaacatca
    24481 ggctgtcatt gacgccattt ttgctggtga tgccgacggg gcgcgtaaag caatgatggc
    24541 gcaccttagt tttgttcaca ccaccatgaa acgattcgat gaagatcagg ctcgccacgc
    24601 acggattacc cgcctgcccg gtgagcataa tgagcattcg agggagaaaa acgcatgatt
    24661 atttccgcag ccagcgatta tcgcgccgca gcgcaacgca ttctgccgcc gttcctgttc
    24721 cactatatgg atggtggtgc atattctgaa tacacgctgc gccgcaacgt ggaagatttg
    24781 tcagaagtgg cactgcgcca gcgtattctg aaaaacatgt ccgacttaag cctggaaacg
    24841 acgctgttta atgagaaatt gtcgatgccg gtagcactgg caccggtggg tttgtgtggc
    24901 atgtatgcgc gtcgtggcga agttcaggca gccaaagcgg cggacgcgca tggcattccg
    24961 tttactctct cgacggtttc cgtttgcccg attgaagaag tcgcgccagc catcaagcgc
    25021 ccaatgtggt tccagcttta tgtactgcgc gatcgcggct ttatgcgtaa cgcgctggag
    25081 cgagcaaaag cagcgggttg ttcgacgctg gttttcaccg tggatatgcc gacaccgggc
    25141 gcacgctacc gtgatgcgca ttcaggtatg agcggcccaa atgcggcaat gcgccgctac
    25201 ttgcaggcgg tgacgcatcc gcaatgggcg tgggatgtgg gcctgaacgg tcgtccacat
    25261 gatttaggta atatctcagc ttatctcggc aaaccgaccg gactggaaga ttacatcggc
    25321 tggctgggga ataacttcga tccgtccatc tcatggaaag accttgagtg gatccgcgat
    25381 ttctgggatg gcccgatggt gatcaaaggg atcctcgatc cggaagatgc gcgcgatgca
    25441 gtacgttttg gtgctgatgg aattgtggtt tctaaccacg gtggccgcca gctggacggt
    25501 gtactctctt ccgctcgtgc gttgcccgct attgcagatg cggtgaaagg tgatatcgcc
    25561 attctggcgg atagcggaat tcgtaacggg cttgatgtcg tgcgtatgat tgcgctcggt
    25621 gccgacacca tactgctggg tcgtgctttc ctgtatgcgc tggcaacagc gggccaggcg
    25681 ggtgtagcta acctgctaaa tctgatcgaa aaagagatga aagtggcgat gacgctgact
    25741 ggcgcgaaat cgatcagtga aattacgcaa gattcgctgg tgcaggggct gggtaaagag
    25801 ttgcctacgg cactggctcc gatggcgaaa gggaatgcgg catagtcgtt tgcgccccct
    25861 caccctaacc ctctccctca gggagagggg accgttcggc gctgtatgta ctccctcact
    25921 ctgaaacgac accgcactct ttttttctcc ctcgcccctc cggggagggc cggggtgagg
    25981 ggaaaaggcc gcacggttca ccaattttct gctatcctgc ccccgcacta agggggcagt
    26041 atgctaaaca tcgtacttta cgaaccagaa attccgccaa atactggcaa catcatccgt
    26101 ctttgcgcta ataccggctt tcgtctacat atcatcgaac cgatgggatt tgcctgggac
    26161 gacaagcgcc tgcgccgcgc ggggctggac tatcacgagt ttaccgccgt tacgcgtcat
    26221 catgactatc gcgcgttcct cgaagcagaa aatccccagc gcctgtttgc cctcaccacg
    26281 aaaggtacgc ctgctcacag cgccgtaagc tatcaggatg gcgattatct gatgttcggc
    26341 ccggaaacac gcggcctgcc agcgagcatt cttgatgccc tgcccgctga acaaaaaatt
    26401 cgcattccga tggtgccgga cagccgcagc atgaatctgt ccaatgcggt gtcggtagtg
    26461 gtgtatgaag cctggcggca gctgggatat ccgggagcgt tgttgagaga ttagttactg
    26521 tatgccggat agatgattac atcgcatccg gcacgatcac aggacattag atcccatccc
    26581 catactcaaa tgtatggtta ataccgttga aatgctggtc catatccatt gatggcttat
    26641 cgctgtctgg tttaccgaca atacgaaccg gaacgccagc ggcggttgta tgcggcggca
    26701 ccggttgcag caccacggaa cctgcgccaa tcttcgcgcc gcgaccaact tcaatattgc
    26761 cgaggatttt cgcgcccgcg ccaatcatca caccttcacg aattttcggg tgacggtcac
    26821 caccagattt acccgtaccg ccaagcgtca cagattgcag aatcgatacg tcgttttcaa
    26881 ttaccgccgt ttcacccaca acgatgcccg tggcgtggtc gagcataatg ccacggccaa
    26941 tttttgctgc cgggtgaata tcgacctgga acgtcacaga aacctggttt tgcagaaaga
    27001 ttgccaatgc gcgacgcccc tgattccaca accagtgacc gatgcgatag gcctgcaagg
    27061 catgaaaacc cttcaggtat aacaacgggg ttgagtattt atcgactgcc gggtcgcggg
    27121 tacgcaccgc ctgaatatca caggccgcag aggcgatcat ttccgggtca gcggcgtagg
    27181 cttcttcaac cacttcacgg atagcaatag caggcataat tggcgatgac agcttgttcg
    27241 ccagcatgta gctcagtgca ctgccaaggt tttcgtgctt gagtagcgtc gcgtggtaaa
    27301 aactggccag cattggctca cagtccgcca gcgttctggc ttcggcttta atattgttcc
    27361 agacaatttc cagttcttca cacgacattg cttactccac acgatgagat aatgaccggc
    27421 ccgttctgcg cgggccgggt catagcggta acaaaggttc cctggggtta gtgactgctg
    27481 cgctcgtcct tgcgtgcacg acctaataat gtcaatgctg cctcgcgcgc gttttttccg
    27541 caatataata cttgataaat ttcctcggtt attggcattt caacgccgaa gcgatgcgcc
    27601 agttcgcgga cttctttcgt attgcggtag ccttccacca cctgaccaat cttctcctgc
    27661 gcgctttgta catccatgcc ctgaccgagc atcataccaa aacggcggtt acgcgactgg
    27721 ttgtcggtac aggtaagcac cagatcgccc agccccgcca tgcccataaa ggtggcagga
    27781 tcggcaccca gcgccgcacc aagacgcgac atttcagcca gcccacgggt gatcagcgcc
    27841 gtacgcgcat tcgcaccaaa accgataccg tcagacatcc ccgcgccaat ggcaatgacg
    27901 tttttcaccg cgccgccaag ctgcacgcca atgaaatccg ggttgctgta aacgcggaaa
    27961 cttttgccgc agtgcagcag ctgctggaga tcatcggcaa aggtctgatc ggtcgaggcc
    28021 agcgaaatag ctgtcggtaa acctgccgcc agttctttcg caaacgttgg gccagagata
    28081 accgccagcg gaatttgatc gcctaaggcc tcacgcgcca cgtcctgtaa cagacgtccg
    28141 gtttccgctt ccagcccttt ggtcgcccac accagacgcg catcaggacg catcagcggt
    28201 ttaatctggc gcagcacttc accaaagaca tggctgggta cgacgacgag aatattacga
    28261 ctggctgcca gcgcagtggc gagatcgctt tcaagatgga gcgtatcggg aaaaggcaca
    28321 tcggggagaa acgcggcgtt acagcggtcg cgttcaagcg ttgcgatatg ttcagggtca
    28381 tggccccaga ggacaacctc gtggccattt cttgccaggg taatggcaag agcggtgccg
    28441 tacgagccgg caccgatcac agtcattgaa gcattacgtt ggttcatcag gcatcctgat
    28501 gttcttcagt accttcgcca gcctgctgct gcaaatagtt catgaacagc gcatcgaagt
    28561 taaccggcgc aaggttcagt tgcgggaatg taccgcggga taccatgctg gtgatgcact
    28621 cacgagcata cgggaacaga atgttcgggc agtatgcgcc caggcaatgt gccatctggg
    28681 tgccttcgat acccgcgatg gagaaaatac cgccctgctg aacttcacac aggaacgcgg
    28741 tttcttcgcc caaggaggcc gttacggtaa cacgcagtac cacttcgtat acgtcatctg
    28801 ccagttggga agatgccgta tccagatcaa gtttaacttc tggttgccaa tctttctgga
    28861 aaacgtgcgg cgcgttcggc gcttcgaaag agatatcctt ggtataaata cgttggatct
    28921 ggaaagtcat ttcagtgttg ttttgttctg acatgtgtag aaaaccctta agtgttgtcc
    28981 ttaaatactg cgcaatgtaa taccaacgtt cacacgttat ttcagcaggg gatccagtcc
    29041 accacgtgca tccaatgcat acaagtcatc acagccgcca atgtgctgtg cgtcaataaa
    29101 aatctgggga accgtggtgc aaccgctgcg tttgatcatc tcttcacgct tggcggcatt
    29161 gccatcgatc ggcaactcct ggaaactcac gcccttgctg ctcagcagtg cttttgcacg
    29221 atggcaatac gggcaggttt ctttggtata gatttcaaca ttggccataa tttagctcct
    29281 gtttttttac cctgaaattt catgttgcag ggcggctgca aaatggagtg tccccagaag
    29341 cttataacaa taagtcgctg ggggcctaat tgcagctaac ggcctgcatc atgaaagacg
    29401 acaggtaaat tatttgccgc gcaccagagg caagttttcg ccagcccagc cagcaacgcc
    29461 ctctttcagc acgaatactt gcgcaaaacc agctttcgtc agcgcgtttg caggctcctg
    29521 gcactgcatg ccagaaccgt ctaccacgat aaccgatttg tctttgtgct tctcaagctc
    29581 accaacattg ttggctttga tttcgctcgg caacagattg atagaacctg cgatatggcc
    29641 tttacggaag tcatcacgct gacgtaaatc cacaaccaca gcgtcttctt tgttgatcag
    29701 acgcgtagct tcaccacgag taatcacctt cactttcgag gtcaggctct taaacgtagt
    29761 cacaagaacc gccaccagta acgcgatcca ggcgatactc agtatgggat gacggccaac
    29821 aaattgcata atttcttgca tggggggtaa caactcccga cgtagtgatt aaaaaaacca
    29881 ggaaaggagt atacctgcgc ggtgcggcaa atacagccag cgcgttaact ggaatgcaat
    29941 tttgcggggc acgacgaaaa aaaagcgcaa atctggcatc ggcttgccgc gagcggtcgt
    30001 atattttgat cttcagaggc tattttatcg attcagctgt agtaaaatta cgcaaatttt
    30061 gactcttgag tatgaggttg tcgcaatgtc ggtttctaaa aaacctatgg tactggtgat
    30121 tctggatggc tatggctatc gtgaagaaca gcaggataac gccattttta gtgctaaaac
    30181 cccggtaatg gatgcactgt gggccaatcg tccgcatacc ctgatcgacg cttccggtct
    30241 ggaagtcggt ctgcctgacc gtcagatggg taactccgaa gtaggccacg ttaacctggg
    30301 tgccggccgc atcgtgtatc aggacctgac tcgtctggac gttgaaatca aagatcgcgc
    30361 tttctttgct aatccagtgc tggctggcgc ggtacataaa gcgaaaaacg caggtaaagc
    30421 agtacacatt atgggtctgc tctccgcagg cggcgtacac agccacgaag atcacatcat
    30481 ggcgatggta gaactggcag ctgaacgcgg cgcagaaaaa atctacctgc acgcattcct
    30541 tgacggtcgc gacaccccgc cgcgcagtgc tgaatcctcg ctgaaaaaat ttgaagagaa
    30601 atttgctgcg ctgggtaaag gccgcgtagc gtccatcatt ggtcgttact acgcgatgga
    30661 ccgcgataac cgttgggatc gcgttgaaaa agcctatgac ctgctgactc tggcgcaggg
    30721 tgagttccag gccgatactg ccgttgctgg tttgcaggca gcttatgctc gcgacgaaaa
    30781 cgacgagttc gtgaaagcga ccgttattcg tgcagaaggt cagccagatg cggcaatgga
    30841 agacggcgat gcgctgattt tcatgaactt ccgtgctgac cgcgcgcgtg aaatcactcg
    30901 tgcattcgtg aacgctgatt tcgatggctt cgcgcgtaag aaagtggtta acgtcgattt
    30961 cgtgatgctg accgaatacg ccgctgacat caaaactgcg gttgcttacc cacccgcttc
    31021 cctggttaac accttcggcg agtggatggc gaaaaacgac aaaactcagt tgcgtatttc
    31081 cgaaaccgaa aaatatgccc acgttacttt cttcttcaac ggtggcgtag aagagtcgtt
    31141 caaaggcgaa gatcgcattc tgatcaactc accgaaagtg gctacctacg atctgcaacc
    31201 ggaaatgagc tccgcagagc tgaccgaaaa actggttgcg gccatcaaga gcggcaaata
    31261 cgacaccatt atctgtaact atccgaacgg cgacatggta ggtcacaccg gggtgatgga
    31321 agcggcggtt aaagcggttg aagcactgga tcactgcgtg gaagaagtcg cgaaagcggt
    31381 tgagtccgtg ggtggacaac tgctgatcac cgctgaccac ggtaacgctg aacagatgcg
    31441 cgatccggca acgggtcagg cacacacggc acacaccaac ctgccagttc cgctgattta
    31501 cgttggtgat aagaacgtga aagcggttgc aggcggcaaa ctttctgaca tcgcgccgac
    31561 catgttgtcg ctgatgggta tggaaatccc gcaagagatg actggtaagc cgctgttcat
    31621 cgtggaataa tccctcccca tgaggggaaa ggcgattaat accatgacac gggccgtgaa
    31681 accgcgcagg tttgcaatca ggcccatcat ctacgccagc gttcttagcg ctggcgtatt
    31741 gttgtgcgcc ttttccgccc acgcggatga gcgtgaccaa ctcaaatcta ttcaggccga
    31801 tatcgccgca aaagagcgcg cggtacgcca aaagcaacaa caacgcgcaa gcctgctcgc
    31861 acaattgaaa aagcaggaag aagcgatctc tgaagccacc cgtaagctgc gcgaaacgca
    31921 aaacacgctc aatcaactca ataaacagat tgatgagatg aacgcgtcga ttgccaaact
    31981 ggagcagcaa aaagccgccc aggagcgcag cctcgccgca caactggatg ccgcattccg
    32041 tcagggcgag cataccggta ttcagctaat tctcagcggt gaagaaagcc agcgtggaca
    32101 gcgtttacag gcttatttcg gctatctcaa ccaggcgcga caagaaacca ttgcccagtt
    32161 gaagcaaacg cgtgaagaag tcaccatgca gcgtgctgaa ctggaagaga aacaaagcga
    32221 gcaacaaacg ctgttatatg aacagcgcgc ccaacaggcg aaactgactc aggctctgaa
    32281 cgagcgtaaa aagacgctgg cggggctgga atcttccatc cagcaaggtc agcagcagct
    32341 aagtgaactg cgtgccaacg aatcccgtct gcgtaacagc attgcccgtg cggaagccgc
    32401 ggcgaaagcg cgtgctgaac gagaagcgcg tgaggcccag gcggttcgcg agcgccagaa
    32461 agaagcgacg cgcaaaggca ccacctacaa accgaccgaa agcgaaaaat cgctgatgtc
    32521 ccgaactggt ggcctgggtg cgccgcgtgg tcaggcattc tggccggttc gcgggccgac
    32581 gctgcatcgc tatggcgagc agctacaggg tgaattacgc tggaaaggga tggttatcgg
    32641 tgcctctgaa ggtactgaag ttaaagcaat tgccgacggt cgagtgattc tggctgactg
    32701 gctgcaaggt tacggtctgg tggtggtggt tgagcacggt aaaggcgaca tgagtcttta
    32761 cggctataat cagagcgcac tggtgagcgt tggttcgcag gttcgcgcgg gccagccaat
    32821 tgcactggtg ggcagcagtg gcggtcaggg tcggccttca ctctatttcg aaattcgccg
    32881 ccagggtcag gcggtcaatc cacagccgtg gttgggaaga taagttttgt ttccatttcg
    32941 tcgtaacgtt cttgcatttg ccgctctgtt ggcgctctcc tcccccgtac ttgctggcaa
    33001 acttgccatc gtcattgatg attttgggta tcgcccgcac aacgaaaacc aggtgctggc
    33061 gatgccttcc gctatctccg tcgctgtatt acccgattca ccgcacgcca gagagatggc
    33121 gaccaaagcg cataacagcg gtcacgaagt gttgattcat ctcccgatgg caccgttgag
    33181 taaacagccg ctggagaaaa atacgctacg cccggagatg agcagcgacg aaattgagcg
    33241 cattattcgt agtgcggtca ataacgtgcc ctatgccgtg gggatcaaca accacatggg
    33301 cagcaagatg acctctaacc tgtttggtat gcagaaagtg atgcaggcgc tggagcgtta
    33361 caatctttac ttcctcgaca gcgtaaccat cggtaatacc caggcgatgc gcgccgcgca
    33421 aggtactggc gtgaaggtga tcaaacggaa ggtgttcctc gacgattcgc agaacgaagc
    33481 ggacatccgt gtgcaattta atcgcgcaat tgatctggcg cgtcgcaacg gttcaaccat
    33541 tgccattggg catcctcacc cttcaacggt acgcgtgcta caacagatgg tttataacct
    33601 gccgccagac attacgctgg tgaaagccag cagcttgctg aatgaaccgc aggttgatac
    33661 ttctacaccg ccgaaaaacg ctgtgcctga tgcaccgcgt aatccgttcc gtggcgtgaa
    33721 gctgtgcaaa ccgaagaaac cgatagaacc tgtttatgct aatcgcttct ttgaagtatt
    33781 aagcgaaagc atcagccaga gcacgctgat cgtttacttc cagcatcagt ggcaaggctg
    33841 gggcaaacag cccgaagcgg cgaagtttaa cgctagcgca aattaaaggc gctacgcgta
    33901 atgcgatggt gcgaccgcgt tttgtcgcgc cattgccata agcggaaaga ccacagtaac
    33961 gcctggtaac cgaccttcac gctgcgcaca ttggtaatca ggcgcttata catacccgga
    34021 agtaaatatc tcggcaatca tccgttggcg ggtaagaata tccggctctt tgcgc
//
LOCUS       DAJTQY010000035        33543 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.035, whole genome shotgun sequence.
ACCESSION   DAJTQY010000035 DAJTQY010000000
VERSION     DAJTQY010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33543
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.035"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            75..1352
                     /gene="waaA"
                     /locus_tag="OO349_001770"
     CDS             75..1352
                     /gene="waaA"
                     /locus_tag="OO349_001770"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HCR5866877.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDAVYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            1360..1839
                     /gene="coaD"
                     /locus_tag="OO349_001771"
     CDS             1360..1839
                     /gene="coaD"
                     /locus_tag="OO349_001771"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HCR5866878.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(1878..2687)
                     /gene="mutM"
                     /locus_tag="OO349_001772"
     CDS             complement(1878..2687)
                     /gene="mutM"
                     /locus_tag="OO349_001772"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HCR5866879.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(2785..2952)
                     /gene="rpmG"
                     /locus_tag="OO349_001773"
     CDS             complement(2785..2952)
                     /gene="rpmG"
                     /locus_tag="OO349_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCR5866880.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            complement(2973..3209)
                     /gene="rpmB"
                     /locus_tag="OO349_001774"
     CDS             complement(2973..3209)
                     /gene="rpmB"
                     /locus_tag="OO349_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HCR5866881.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            complement(3426..4094)
                     /gene="radC"
                     /locus_tag="OO349_001775"
     CDS             complement(3426..4094)
                     /gene="radC"
                     /locus_tag="OO349_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HCR5866882.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            4266..5486
                     /gene="coaBC"
                     /locus_tag="OO349_001776"
     CDS             4266..5486
                     /gene="coaBC"
                     /locus_tag="OO349_001776"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HCR5866883.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            5464..5922
                     /gene="dut"
                     /locus_tag="OO349_001777"
     CDS             5464..5922
                     /gene="dut"
                     /locus_tag="OO349_001777"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HCR5866884.1"
                     /translation="MMKKIDVKILDPRVRKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            6029..6625
                     /gene="slmA"
                     /locus_tag="OO349_001778"
     CDS             6029..6625
                     /gene="slmA"
                     /locus_tag="OO349_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HCR5866885.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(6662..7303)
                     /gene="pyrE"
                     /locus_tag="OO349_001779"
     CDS             complement(6662..7303)
                     /gene="pyrE"
                     /locus_tag="OO349_001779"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HCR5866886.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(7369..8085)
                     /gene="rph"
                     /locus_tag="OO349_001780"
     CDS             complement(7369..8085)
                     /gene="rph"
                     /locus_tag="OO349_001780"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HCR5866887.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            8212..9075
                     /locus_tag="OO349_001781"
     CDS             8212..9075
                     /locus_tag="OO349_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HCR5866888.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMKSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            9296..10120
                     /gene="dinD"
                     /locus_tag="OO349_001782"
     CDS             9296..10120
                     /gene="dinD"
                     /locus_tag="OO349_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HCR5866889.1"
                     /translation="MNEHHQPFEEIKLINANGAEQWSARQLGKLLGYSEYRHFIPVLT
                     RAKEACENSGHTIDDHFEEILDMVKIGSNAKRALKDIVLSRYACYLVVQNGDPAKPVI
                     AAGQTYFTIQTRRQELADDEAFKQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAT
                     DFAIFQNHGYQGLYGGLDQKAIHQLKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDSVNSKQQANTTHFDVGSKVRQTIQELGGTIPEELPTPQVSIKQLENSVKITEKK"
     gene            10411..11028
                     /locus_tag="OO349_001783"
     CDS             10411..11028
                     /locus_tag="OO349_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HCR5866890.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(11025..12707)
                     /gene="ligB"
                     /locus_tag="OO349_001784"
     CDS             complement(11025..12707)
                     /gene="ligB"
                     /locus_tag="OO349_001784"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HCR5866891.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNPLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARNQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            12965..13588
                     /gene="gmk"
                     /locus_tag="OO349_001785"
     CDS             12965..13588
                     /gene="gmk"
                     /locus_tag="OO349_001785"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HCR5866892.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            13643..13918
                     /gene="rpoZ"
                     /locus_tag="OO349_001786"
     CDS             13643..13918
                     /gene="rpoZ"
                     /locus_tag="OO349_001786"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCR5866893.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQELEAAELQAVTAIAEGRR"
     gene            13937..16045
                     /gene="spoT"
                     /locus_tag="OO349_001787"
     CDS             13937..16045
                     /gene="spoT"
                     /locus_tag="OO349_001787"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HCR5866894.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            16052..16741
                     /gene="trmH"
                     /locus_tag="OO349_001788"
     CDS             16052..16741
                     /gene="trmH"
                     /locus_tag="OO349_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HCR5866895.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            16747..18828
                     /gene="recG"
                     /locus_tag="OO349_001789"
     CDS             16747..18828
                     /gene="recG"
                     /locus_tag="OO349_001789"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HCR5866896.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARV
                     VAEIEHDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(18862..20067)
                     /gene="gltS"
                     /locus_tag="OO349_001790"
     CDS             complement(18862..20067)
                     /gene="gltS"
                     /locus_tag="OO349_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HCR5866897.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            20347..21738
                     /gene="xanP"
                     /locus_tag="OO349_001791"
     CDS             20347..21738
                     /gene="xanP"
                     /locus_tag="OO349_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HCR5866898.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            21859..23568
                     /locus_tag="OO349_001792"
     CDS             21859..23568
                     /locus_tag="OO349_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCR5866899.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPAPLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANAPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSTE
                     PVR"
     gene            complement(23621..25939)
                     /gene="yicI"
                     /locus_tag="OO349_001793"
     CDS             complement(23621..25939)
                     /gene="yicI"
                     /locus_tag="OO349_001793"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HCR5866900.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPPWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPIDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            complement(25949..27332)
                     /locus_tag="OO349_001794"
                     /pseudo
     CDS             complement(25949..27332)
                     /locus_tag="OO349_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
     gene            27625..27719
                     /locus_tag="OO349_001795"
     tRNA            27625..27719
                     /locus_tag="OO349_001795"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27659..27661,aa:Sec,seq:tca)
     gene            28020..29204
                     /locus_tag="OO349_001796"
     CDS             28020..29204
                     /locus_tag="OO349_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5866901.1"
                     /translation="MALTDIKVKTAKPKDKPYKLADGGGMYLLINTNGSKYWRMKYRF
                     AGKEKMLSIGVYPDVTLADAREKRSEARKLLAAGGDPREAKKEEKIAQQMSLKNTFEA
                     IAREWHQLKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMKLLEALRKMEKRGA
                     LEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTANELPHFLND
                     LAGYTGSIITKTATQIIMLTGVRTQELRFARWEDIDFETKLWEIPAEVMKMKRPHIVP
                     LSEQVIMLFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKGRLTGHGFRH
                     TMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYADYIDSLSSR
                     ES"
     gene            29616..30659
                     /locus_tag="OO349_001797"
     CDS             29616..30659
                     /locus_tag="OO349_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCR5866902.1"
                     /translation="MNDRLCFEVHDNKGYFVFPDTWFGPLLGEFEEVLDAYDADEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNAALKGLAAGNRIIPESFCG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPENNQGARWLLGSEL
                     LRTGDHERAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNLHPFPLAVWHDFSGSLDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERSE
                     IIKCAEMLIQEDDFEICESILRQQEHLWKRIDKTLSEEIVQKCRNMNGEYIWPWILPF
                     SAAGMKHSSIQYQ"
     gene            31174..31602
                     /locus_tag="OO349_001798"
                     /pseudo
     CDS             31174..31602
                     /locus_tag="OO349_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
     gene            complement(31632..32783)
                     /locus_tag="OO349_001799"
                     /pseudo
     CDS             complement(31632..32783)
                     /locus_tag="OO349_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            32848..33502
                     /locus_tag="OO349_001800"
                     /pseudo
     CDS             32848..33502
                     /locus_tag="OO349_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164328.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT         8403 a         8816 c         8267 g         8057 t
ORIGIN      
        1 aacgggaaaa gtaatggtaa agccacagct aaatacatag aatccccagc acatccataa
       61 gtcagctatt tactatgctc gaattgcttt acaccgccct tctctacctt attcagccgc
      121 tgatctggat acggctctgg gtgcgcggac gtaaggctcc ggcctatcga aaacgctggg
      181 gtgaacgtta cggtttttac cgccatccgc taaaaccagg cggcattatg ctgcactccg
      241 tctccgtcgg tgaaactctg gcggcgatcc cgttggtgcg cgcgctgcgt catcgttatc
      301 ctgatttacc gattaccgtt acaaccatga cgccaaccgg ttcggagcgc gtacaatcgg
      361 ctttcgggaa ggatgttcag cacgtttatc tgccgtatga cctgcccgat gcgctcaacc
      421 gtttcctgaa taaagtcgac cccaaactgg tgttgattat ggaaaccgaa ctatggccta
      481 acctgattgc ggcgctacat aaacgtaaaa ttccgctggt gatcgctaac gcgcgactct
      541 ctgcccgctc ggccgcaggt tatgccaaac tgggtaaatt cgtccgccgc ttgctgcgtc
      601 gtattacgct gattgctgcg caaaatgaag aagatggtgc acgttttgtg gcgctgggcg
      661 caaaaaataa ccaggtaacc gttaccggta gcctgaaatt cgatatttct gtaactccgc
      721 agttggctgc taaagccgtg acgctacgcc gccaatgggc accacaccgc ccggtatgga
      781 ttgccaccag cactcatgaa ggtgaagaga gcgtggtcat tgccgcacat caggcattgt
      841 tacagcaatt cccgaattta ttgctcatcc tggtaccccg tcatccagaa cgcttcccgg
      901 atgcgattaa ccttgtccgc caggctggac taagctatat cacacgctct tcaggggaag
      961 tcccctccac cagcacgcag gttgtggttg gcgatacgat gggcgagttg atgttactgt
     1021 acggcattgc cgatctcgcc tttgttggcg gttcactggt tgaacgtggt gggcataatc
     1081 cgctggaagc tgccgcacac gctattccgg tattgatggg gccgcatact tttaacttta
     1141 aagacatttg cgcgcggctg gagcaggcaa gcgggcttat taccgttacc gatgccacta
     1201 cgcttgcaaa agaggtttcc tctttactca ccgacgccgt ttaccgtagt ttctatggcc
     1261 gtcatgccgt tgaagtactg tatcaaaacc agggcgcgct acagcgtctg cttcaactgc
     1321 tggaacctta cctgccaccg aaaacgcatt gaggttgtta tgcaaaaacg ggcgatttat
     1381 ccgggtactt tcgatcccat taccaatggt catatcgata tcgtgacgcg cgccacgcag
     1441 atgttcgatc acgttattct ggcgattgcc gccagcccca gtaaaaaacc gatgtttacc
     1501 ctggaagagc gtgtggcact ggcacagcag gcaaccgcgc atctggggaa cgtggaagtg
     1561 gtcgggttta gtgatttgat ggcgaacttc gcccgtaatc aacacgctac ggtgctgatt
     1621 cgtggcctgc gtgcggtggc ggattttgaa tatgaaatgc agctggcgca tatgaatcgc
     1681 cacttaatgc cggaactgga aagtgtgttt ctgatgccgt cgaaagagtg gtcgtttatc
     1741 tcttcatcgt tggtgaaaga ggtggcgcgc catcagggcg atgtcaccca tttcctgccg
     1801 gagaatgtcc atcaggcgct gatggcaaaa ttagcgtagc gtttatgccg gatggtatgc
     1861 catccggctc gcatgaatta cttctggcac tgccgacaat aaaacgttgc ccgctgcgca
     1921 tgtttagtcg ccacaatcgg cgtaccgcac acccgacacg gctcaccttt tcgcccgtaa
     1981 acctgcaatt cctgcgcgaa atagcccggt ttaccatcac tttgcagaaa atctttcagc
     2041 gttgtaccac cctgctcaat cgaacgcagc aacaccgctt taatcacccg agctaacaat
     2101 tcacactctg ccagcgacag tgatgacgcc agccgatccg gatggatccc cgccgcaaac
     2161 agtgattcgc tggcatagat attccctacc cctaccacca gcttgttatc catcagccac
     2221 ggtttaatcg ccgttttttt cttcgcgcac ttctgatgca gatactcacc attgaaatcg
     2281 tcgctaagcg gctccggtcc aagatgggcc agcacattat gcccttccag ctctttggtc
     2341 cacagccagg caccaaagcg gcgcggatcg gtgtagcgca gcactttacc gttgctcatc
     2401 accaaatcca catggtcatg cttttcaggg ggaagttctt ctggaaggat gcgcaggctg
     2461 ccagacatcc ccaaatgaat gataatccag ccctcaggca gctccagcag cagatattta
     2521 gcccgccgct gcacgctaag cactggttga tcgcttaatc ggtagatctc ttctgaaacc
     2581 ggccagcgca agcgtccgtt gcgcaccacc gcatgaagaa tggttgcacc aacgagatgc
     2641 ggttctatgc cgcgacggct ggtttcaact tcgggtaatt caggcatagc atctccagga
     2701 atgaacagat gcaatcaata tgggggcaag cagataacaa aaaaccccgc cggagcgagg
     2761 tttttcgtta catcaaagcg agaattattt gattttcgct tctttgtaga tcacgtgctg
     2821 acgaacaact ggatcgaatt ttttcagttc cagtttttcc ggcttagtac gtttgttctt
     2881 cgtagtggta tagaagtgac cagtaccagc agaagaaacc agcttgattt tctcacgaat
     2941 acctttagcc atgatttatt tcctctaagt acttagtact tttcgccacg ggcacgcagt
     3001 tcagccagaa ctgtatcgat gcctttttta tcgattacac gcataccttt agcagatacg
     3061 cgcagggtga caaaacgctt ctcgctctca acccagaaac ggtgagagtg caggttcggc
     3121 aggaaacggc gtttagtcgc gttcagtgcg tgggaacggt tgttaccggt caccggacgc
     3181 ttgccagtaa cttggcagac tcgggacatg tctattctcc aaaaatcaaa ttagctcgag
     3241 cttcgtatgg ggtattggcg cctcgtcagg ctttacagcc cggtcatcgc agttctatgt
     3301 gaactctcga ttgccaggcc caaatgccaa acccgagatt ctcaaaggtg gcgtagtata
     3361 cgctgactca gcgatgtgct caagtcccga acagacaaag atcccgaagg atcgcgcata
     3421 gcgggttaaa tccagccgcg ttcggcaaaa gaaacatact ctccacgccc aatcacgata
     3481 tggtcgagca cgcgtaaatc catgaactga caactcttta ttatccgttc agtaatgagt
     3541 ttatccgctt tactgggttc agcacaaccc gaagggtgat tatgtgcaag gatcagcgcc
     3601 gaggcgttta tttttatcgc ttcgcggata atttcccgag gatggacttc aacatggttt
     3661 agcgtgccgg aaaaaagacg gctatgcgtt ataacccggt gttgtgagtc gagaaagatc
     3721 accataaaga tctcccgctc ctcacccgtg agctggcttt gtaaaaattc ccgcgtcatc
     3781 tccgggctga gtaaagggct ttcttcacgc atccgcacgt tgtagtaacg tcgcgccagt
     3841 tcagcaatcc cctttaactg ggcaaatttc gctacgccaa ttccatgaac accactaaat
     3901 tgttcatatt cagaggttaa caagccataa agagagccga aattctccag catctctttt
     3961 gccagggtta atacatcttt accgcgcgtt ccggtacgca gaaatagcgc cagcagctcg
     4021 acatccgtta aggcgctaat accaaacttc agcatttttt cgcgcggcat caacagctgt
     4081 gcattgtttt tcaccttcac ctcctttgtg gtgctcgcat cctgtcatag ccgttccgtt
     4141 aaatcgacgg ccagttttca atcctggaaa gagcctcgca aagtgaatct caggtgaagg
     4201 cacgacaaca aaagtattgt gataaaatcg ccaacttctg gtgtcaccca acaggaaaaa
     4261 tcatcatgag cctggccggt aaaaaaatcg ttctcggcgt tagcggcggt attgctgcct
     4321 ataaaacccc tgaactggtg cgtcgtttgc gcgatcgcgg ggccgacgtc cgcgtagcca
     4381 tgaccgaagc ggcaaaagcc tttatcaccc cacttagctt gcaggcggtt tctggttatc
     4441 ccgtttccga cagtctgctt gacccggcag ccgaagccgc tatgggccat attgagctgg
     4501 gtaaatgggc tgatttagtg attctcgccc ctgccacggc agatttgatt gcccgtgttg
     4561 ctgccggaat ggcgaatgac ctggtatcga cgatttgtct ggctacacct gcgcctgtag
     4621 ccgtgctccc cgccatgaac cagcagatgt accgtgccgc tgccacgcag cataatttag
     4681 aggtgcttgc ttcccgtggt ttgctcatct gggggccaga cagtggcagt caggcttgtg
     4741 gtgatatcgg tcctggacga atgctcgatc cgttaaccat tgtggatatg gcggtggcgc
     4801 atttttcgcc cgtcaacgac ctgaaacatc tgaacattat gattaccgcc ggtccgacgc
     4861 gtgaaccgct cgatccggtg cgttatatct ctaatcacag ctccggcaag atgggttttg
     4921 ctatcgccgc cgctgctgcc cgtcgtggcg cgaacgtcac gctggtatca ggtccagttt
     4981 cactaccgac gccaccgttt gttaaacgtg ttgatgtgat gaccgcgctg gaaatggaag
     5041 ccgccgtgaa tgcttctgta cagcagcaaa atatttttat cggttgcgcc gccgtggcgg
     5101 attatcgcgc agctaccgtg gccccagaaa aaatcaaaaa gcaggccacg cagggtgatg
     5161 aattaacaat aaaaatggtt aaaaaccccg atatcgtcgc aggcgttgcc gcactaaaag
     5221 accatcgacc ctacgtcgtt ggatttgccg ccgaaacaaa taatgtggaa gaatacgccc
     5281 ggcaaaaacg tatccgtaaa aaccttgatc tgatctgcgc gaacgatgtt tcccagccaa
     5341 ctcaaggatt taacagcgac aacaacgcat tacacctttt ctggcaggac ggagataaag
     5401 tcttaccgct tgagcgcaaa gagctccttg gccaattatt actcgacgag atcgtgaccc
     5461 gttatgatga aaaaaatcga cgttaagatt ctggacccgc gcgttaggaa ggaatttccg
     5521 ctcccgactt atgccacctc tggctctgcc ggacttgacc tgcgtgcctg tctcgacgac
     5581 gccgtagaac tggctccggg tgacactacg ctggttccga ccgggctggc gattcatatt
     5641 gccgatcctt cactggcggc aatgatgctg ccgcgctccg gattgggaca taagcacggt
     5701 atcgtgcttg gtaacctggt aggattgatc gattccgact atcagggcca gttgatgatt
     5761 tccgtgtgga accgtggtca ggacagcttc accattcaac ctggcgaacg catcgcccag
     5821 atgatttttg ttccggtagt acaggctgaa tttaatctgg tggaagattt cgacgccacc
     5881 gaccgcggtg aaggcggctt tggtcactct ggtcgtcagt aacacatacg catccgaata
     5941 acgtcataac atagccgcaa acatttcgtt tgcggtcata gcgtgggtgc cgcctggcaa
     6001 gtgcttattt tcaggggtat tttgtaacat ggcagaaaaa caaactgcga aaaggaaccg
     6061 tcgcgaggaa atacttcagt ctctggcgct gatgctggaa tccagcgatg gaagccaacg
     6121 tatcacgacg gcaaaactgg ccgcctctgt cggcgtttcc gaagcggcac tgtatcgcca
     6181 cttccccagt aagacccgca tgttcgatag cctgattgag tttatcgaag atagcctgat
     6241 tactcgcatc aacctgattc tgaaagatga gaaagacacc acagcgcgcc tgcgtctgat
     6301 tgtgttgctg cttctcggtt ttggtgagcg taatcctggc ctgacccgca tcctcactgg
     6361 tcatgcgcta atgtttgaac aggatcgcct gcaagggcgc atcaaccagc tgttcgagcg
     6421 tattgaagcg cagctgcgcc aggtattgcg tgaaaagaga atgcgtgagg gtgaaggtta
     6481 caccaccgat gaaaccctgc tggcaagcca gatcctggcc ttctgtgaag gtatgctgtc
     6541 acgttttgtc cgcagcgaat ttaaataccg cccgacggat gattttgacg cccgctggcc
     6601 gctaattgcc gcgcagttgc agtaatatga cgccggatga cttttcatcc ggcgagtttc
     6661 tttaaacgcc aaactcttcg cgataggcct taaccgccgc cagatgttcc gccatttccg
     6721 gcttctcttc cagataggca atcagatctt tcagggtgat gatagagatc actttgcagt
     6781 tgtaatcacg ctcaacttcc tgaatcgccg aaatctcgcc gcgcccgcgt tcctgacgat
     6841 cgagcgaaat caacacgcca gcaagcgtcg cgccattggc ctgaataatc tccatcgact
     6901 cgcgaatcgc cgttccggcg gtgatcacat catctaccag cattacgcgt ccttgtaacg
     6961 cgctaccaac cagattgccg ccttcaccgt ggtcttttgc ttctttgcgg ttaaagcagt
     7021 acggcaggtc caggtcgtga tgctccgcca gtgccacagc ggttgtggtg gcaatcggga
     7081 tccctttgta agcagggcca aacagcagat cgaactcaat gccggaatcc accaacgctt
     7141 cagcgtaaaa acggcctaac agtgccagat cgcgcccggt attaaacagc ccggcgttga
     7201 agaaataggg gcttttgcgc ccggatttca gcgtaaactc gccaaacttt aacacctgct
     7261 tgctaagcgc aaattcaata aactggcgct gatatggttt catgccttcg ctcctcatct
     7321 tacttttcta cagacaaaaa aaaggcgact catcagtcgc cttaaaaatc agtttgccag
     7381 cgccgccttc tgcgtcgcta caatggattc gattccccct cgggccagcg ccaacaaggt
     7441 gagtagctct tcatgggtga acggctcgcc ttctgccgtc ccctgcactt caatgatgcg
     7501 cccgtcttcg gtcatcacta cgttcatgtc ggtctctgcg gctgagtctt caacgtattc
     7561 cagatcgcaa attgcttcac cgtttacaat accgacagaa actgcggcta ccatcccttt
     7621 catcgggttg gttttcagct tgccgttttc caccagcttc tgtagcgcat ctgccagcgc
     7681 cacgcaggca cccgtaatcg acgcggtacg agtgccacca tcagcctgaa gcacgtcgca
     7741 gtccagcgta atggtgaact cacccagcgc tttcaaatct actgccgcgc gaagagcacg
     7801 ggcgatcaga cgctggattt ccattgtgcg tccaccctgc ttacctttcg ccgcttcacg
     7861 agcgttacgg gtgtgggtag aacgtggcag catgccgtac tctgcggtga tccagccctg
     7921 gccctgacct ttcaggaagc gcggcacgcc ttcttcaata gaggcggtac acaatacttt
     7981 ggtatcgcca aattcgacca gcaccgagcc ttctgcatgt tttgtatagt tacgagtcag
     8041 ggtaacggga cgcacctgat tattgctacg gcctgctgga cgcatattga aatctccggc
     8101 ttgaaacaaa tgtggctgcg cattatacgg acttccggcg gttattccta tcctgacaag
     8161 gcatcgatgg ctataatcct tccacctctc cttttataaa caggaacgtc tatgatccgc
     8221 agtatgaccg cctacgcccg gcgtgaaatc aagggtgaat gggggagcgc aacctgggaa
     8281 atgcgctcgg taaaccagcg ttatctggaa acttactttc gtctgccgga gcagttccgt
     8341 agccttgaac ctgtcgttcg cgagcgtatt cgttctcgcc tgacgcgcgg taaagtggaa
     8401 tgtaccctgc gctatgagcc agatgttagc gcacaaggtg agctgatcct caatgaaaaa
     8461 ctggctaaac agctggtaac tgccgcgaac tgggtaaaaa tgaagagtga cgaaggggaa
     8521 atcaacccgg ttgatattct acgctggccg ggcgtgatgg cagcccagga gcaggatctc
     8581 gacgcgattg ccgctgaaat tctcgcggcg ttggatggta cgctggacga ctttatcgtc
     8641 gcgcgcgaaa ccgaaggtca ggcactgaaa gcattgatcg agcagcgtct ggaaggcgtc
     8701 accgccgaag tggtcaaagt ccgcgcccat atgccggaaa ttctgcaatg gcagcgtgag
     8761 cgtctggtcg cgaagctgga agatgctcag gttcagttgg aaaacaaccg tctggagcag
     8821 gagttggttc tgctggcaca acgaattgac gttgccgaag agctggaccg cctcgaagcg
     8881 catgttaaag agacgtacaa cattctgaag aaaaaagaag cggttggtcg tcgtctggac
     8941 tttatgatgc aggagttcaa ccgtgagtcg aacactcttg cgtcgaaatc tatcaatgcc
     9001 gaagtgacaa actccgccat cgagctgaaa gtgttgattg agcagatgcg cgagcagatt
     9061 cagaatattg agtaactcgt aacaccaatt ctgacttttc agccttcaca aatgccgccc
     9121 actcaaacag agcggcattt ttcttccccg caacttcaat tctgttttgc gtgcctgctc
     9181 cagattttgc gatgtttttt tgcccagcac actgagaacg tgagatactc acaactgtat
     9241 ataaatacag ttacagattt actttctttg caatcgatat cacatggagt gggcaatgaa
     9301 cgaacatcat caaccttttg aagagataaa actgattaat gcaaacggag cagaacaatg
     9361 gtcagcaaga caacttggga aactactggg ttattcagag tatcgtcact ttatacctgt
     9421 attaacgcgc gccaaagagg cctgtgagaa cagtggtcac acaattgatg accatttcga
     9481 ggagatcctc gatatggtca aaattggctc aaatgccaaa agagcattaa aagacatcgt
     9541 actctcccgc tatgcctgtt acctggtagt acaaaacggc gaccccgcga aaccggtcat
     9601 tgcggcaggg cagacttatt ttactatcca gacccgacgg caggagcttg ctgacgatga
     9661 agcatttaag caacttcgtg aagacgaaaa gcgtctgttt ctgagaaatg agttgaaaga
     9721 acataacaaa caattggttg aggcagcaca gcaagctggg gtagcgacag ctactgattt
     9781 cgccatattt cagaatcatg gttaccaggg gctgtatggt ggattagatc agaaagccat
     9841 ccaccaactg aaggggctga aaaagagtca gaagatcctg gatcatatgg gttcaacaga
     9901 actggcggct aatctctttc gagctaccca aacagaagaa aaactcaagc gggatagcgt
     9961 gaattcaaaa cagcaagcta ataccacaca ctttgacgtg ggtagcaagg tgaggcaaac
    10021 cattcaggaa cttggcggaa ccatacctga ggagttgccg accccgcagg tcagcatcaa
    10081 gcagttggaa aacagtgtaa aaattacaga gaagaaatag cagaaacgaa aaactctttt
    10141 tgttgattga gacacccgat gcgtaagcca aggtccaggt gcagtgaaca caatggctaa
    10201 acattgcacc tttcttttcc cccctcagtt ttaacctatt ttttcttatg cattttctca
    10261 gacaagaagt cagaagaatg catctacgct acagaaaata gcgatttcac ataactaaaa
    10321 gttatcaatt tcctctcacc ttaaaaaata tcaatcgtga caatgcgcac aaatcgctac
    10381 cctgccagac agatttttag ggagagaacc atgctgttac acattttgta tttggttggc
    10441 attactgccg aagccatgac gggggcgctg gcggccgggc gacggcgcat ggatacattt
    10501 ggcgtaatta ttattgcaac cgccaccgca attggcggag ggtcagtgcg cgatattctg
    10561 ctgggccact atccgctcgg ctgggtcaaa cacccggaat atgtgattat cgtcgctacc
    10621 gccgcagtac tcaccactat cgttgctcct gtaatgcctt acctgcgcaa agtgtttctg
    10681 gtgctcgatg cgctcggact ggtggtcttt tctatcatcg gcgcacaggt tgcactggat
    10741 atggggcacg gcccaattat tgccgttgtc gcggcggtaa ccaccggtgt atttggcggc
    10801 gttttgcgcg atatgttctg taaacgcatt ccgctggtct ttcaaaaaga gttgtatgcc
    10861 ggtgtctcat ttgcctccgc cgtgctgtac atcgcgctgc aacactatgt ttctaaccat
    10921 gatgtggtga ttatctccac cctggtattc ggcttttttg ctcgtttact ggcgctacgt
    10981 cttaagctgg gattaccggt cttttactac agccacgaag gtcactaagg ttcaaaacct
    11041 gtgatctgct gggcagccag ccaactgccc agcttcttga tttgcgcatt ttccttccat
    11101 tcaataacct gtctggcgcg cccagatcca gtccccggca gctgctgcca gaactgctcc
    11161 gtgctaaata aaagttgcga ccaggaccgt tcatcactgg cattaagcgc cgcccgggtt
    11221 agcggtattc ccattgccat cacccagcga gtaaaaggct ggttacgagc cagattaaac
    11281 tgatgccata actgcgcact tttacttttc gcgatccccg gcgtgttctg taattgctct
    11341 ggcgttaata aaagccagga aaagatatgc tcaaagcgat gagtctgatg cagcgcgcgc
    11401 caaccggcct caccaatgcc atccagcccc agaacctgtt ttgaccccag ccagactaag
    11461 cgtgaaatga actgttcctg acaaacatca gaagcaaagt agcaggtcaa cgggttaaag
    11521 cggttttctg gtggtgtcgg ttttgtacgt tctgcaccgc gccagaccac atcatcaatg
    11581 cgaggaatac cctgaccggc aaggctgacg aggatctgat caccaggcgc aatatcccac
    11641 tcctgccagc gcctgacgga accaatattc acccgctgga cttttttatc atccagcatg
    11701 acaggtgcga gtgacgcaac caccgatatt ttaccgctct tacccaccgc aaactgaatc
    11761 gccttcactt cggcaacctg agctacaggt tgatatttcc aggccaccag ccactccgcc
    11821 tggcccggta gccaatgacg ggattcgggt tccttagccc ctcgcacaac cacgccatcg
    11881 gtgacgaagg gtaatttcgc tttccaccac tcattgcgta cgcgcgcaac ctcatcagca
    11941 tttttaaccg cacgggtata cctctgcgtt agagtaaaac ctgcggtagc cagctccttt
    12001 aaacgatcgg tcattaactg cggtccatcc ggccatgccc agacaaaaac agccagtgag
    12061 tttagcgtgt cgctattccc ctggcgcatc atcaggccag caacttttgc gcgggcattt
    12121 attcccccca tttgttgttg gatatgcccc tcgcgctgga gaaatatttc cccctgaagc
    12181 gtactgttgg ctaaaggccc gctaacggtt tgcggcacag cggaaattaa gctaactttc
    12241 tgcgtccagt cctcgccttt caggccatta ccgcgactga ttgctttgtt cagtttcccg
    12301 tcccgataaa ccagggttac cgcaacgcca tcaacttttg gctgcaccca aagatcgcta
    12361 cgttctcgca tccacagact taatgcgttt ttatccgcca ttttgcgtac acccgtatgg
    12421 gcaaccggat gcataaccgc accatttaaa ggcggcatca tcacgtcgcg gggctcgctc
    12481 acaaagcagc gttgccactg cgtaagacgg gcgcttaact gatcgtaaac accgtcttcc
    12541 acctcacttt ttccttcctt ccagtagtca tcgtcccact gttttatttg ctgttgcagg
    12601 cgggaaattt cttcctgtgc tctggctggc gaccaggccg gacagaccgc ccacacagat
    12661 gattgccagc acaagatact tattaatatc gccatccata ctttcatctt cacctccgct
    12721 gtagtcaggc tggcagatat acaacgtggt gaaggcaaag ccgagtggca aaaacggagt
    12781 ctgcgaggac gcttcctgag aatcgtcttt attgcagtaa atcacaggca aatgcggaaa
    12841 cagctacgca aaacgcaaca actttgcgca aaaagtgtga gcaagggcta cgtcacatgg
    12901 ccgcgccgtg tataataagc tcgtatgtag gctttatttc gctaatcaca tacgaaagat
    12961 actcatggct caaggcacgc tttatattgt ttctgccccc agtggcgcgg gtaaatccag
    13021 cctgattcag gctttattaa aaacccaacc gttgtatgac acccaggttt ctgtttcaca
    13081 caccacacgc caaccgcgtc ctggtgaagt ccacggtgaa cattatttct ttgttaatca
    13141 tgatgaattt aaagaaatga ttagcagaga tgcgttcctc gaacacgcag aagtttttgg
    13201 taattactat ggcacttcgc gtgaggccat tgagcaagta ctggcgaccg gtgtcgatgt
    13261 ttttctcgat atcgactggc agggcgcgca gcaaattcgc cagaagatgc cgcacgcgcg
    13321 gagtatcttt attttaccgc cgtccaaaat tgaactggac cgccgtctac gcggtcgcgg
    13381 tcaggacagc gaagaggtca ttgcaaagcg tatggcgcaa gctgttgcag aaatgagcca
    13441 ttacgccgaa tatgattatc tgattgtgaa tgatgacttc gataccgcgt tgaccgattt
    13501 gaagaccatt attcgcgccg aacgtctgcg catgagccgc caaaagcagc gtcatgacgc
    13561 tttaatcagc aaattgttgg cagactgaac ctgatttcag tatcatgccc agtcatttct
    13621 tcacctgtgg agctttttaa gtatggcacg cgtaactgtt caggacgctg tagagaaaat
    13681 tggtaaccgt tttgacctgg tactggtcgc cgcgcgtcgc gctcgtcaga tgcaggtagg
    13741 cggaaaggat ccgctggtac cggaagaaaa cgataaaacc actgtaatcg cgctgcgcga
    13801 aatcgaagaa ggtctgatca acaaccagat cctcgacgtt cgcgaacgcc aggaacagca
    13861 agagctggaa gccgctgaat tacaagccgt taccgctatt gctgaaggtc gtcgttaatc
    13921 acaaagcggg tcgcccttgt atctgtttga aagcctgaat caactgattc aaacctacct
    13981 gccggaagac caaatcaagc gtctgcggca ggcgtatctc gttgcacgtg atgctcacga
    14041 ggggcaaaca cgttcaagcg gtgaacccta tatcacgcac ccggtagcgg ttgcctgcat
    14101 tctggccgag atgaaactcg actatgaaac gctgatggcg gcgctgctgc atgacgtgat
    14161 tgaagatact cccgccacct accaggatat ggaacagctt tttggtaaaa gcgtcgccga
    14221 gctggtagag ggggtgtcga aacttgataa actcaagttc cgcgataaga aagaggcgca
    14281 ggccgaaaac tttcgcaaga tgattatggc gatggtgcag gatatccgcg tcatcctcat
    14341 caaacttgcc gaccgtaccc acaacatgcg cacgctgggc tcacttcgcc cggacaaacg
    14401 tcgccgcatc gcccgtgaaa ctctcgaaat ttacagcccg ctggcgcacc gtttaggtat
    14461 ccaccacatt aaaaccgaac tcgaagagct gggttttgag gcgctgtatc ccaaccgtta
    14521 tcgcgtaatc aaagaagtgg tgaaagccgc gcgcggcaac cgtaaagaga tgatccaaaa
    14581 aatcctctcg gaaatcgaag ggcgtttgca ggaagcggga attccctgcc gcgtcagtgg
    14641 tcgcgagaag catctttatt cgatttactg caaaatggtg ctcaaagagc agcgttttca
    14701 ctcaatcatg gacatctacg ctttccgcgt gatcgtcaat gattccgaca cctgttatcg
    14761 cgtgctcggg caaatgcata gcctgtacaa gccgcgtccg ggtcgcgtga aagactacat
    14821 cgccattcca aaagcgaacg gctatcagtc tttgcacacc tcgatgatcg gcccgcacgg
    14881 tgtgccggtt gaggtccaga tccgtaccga agatatggat cagatggcgg agatgggtgt
    14941 tgccgcgcac tgggcttata aagagcacgg cgaaaccagc accaccgcgc aaatccgcgc
    15001 ccagcgctgg atgcaaagcc tgctggagct gcaacagagc gccggtagtt cgtttgaatt
    15061 tatcgagagc gttaaatccg atctcttccc ggatgagatt tacgttttca caccggaagg
    15121 gcgaattgtc gagctgcctg ccggtgcaac gcccgtcgac ttcgcttatg cagtgcatac
    15181 cgatatcggt catgcctgcg tgggcgcacg cgttgaccgc cagccttacc cgctgtcgca
    15241 gccgcttacc agcggtcaaa ccgttgaaat cattaccgca ccgggcgctc gcccgaatgc
    15301 cgcgtggctg aactttgtcg ttagctcaaa agcgcgcgcc aaaattcgtc agttgctgaa
    15361 aaacctcaag cgtgatgatt ctgtaagcct gggccgtcgt ctgctcaacc atgctttggg
    15421 tggtagccgt aagctgaatg aaatcccgca ggaaaatatt cagcgcgagc tggatcgcat
    15481 gaagctggca acgcttgacg atctgctggc agaaatcgga cttggtaacg caatgagcgt
    15541 ggtggtcgcg aaaaatctgc aacatgggga cgcctccatt ccaccggcaa cccaaagcca
    15601 cggacatctg cccattaaag gtgccgatgg cgtgctgatc acctttgcga aatgctgccg
    15661 ccctattcct ggcgacccga ttatcgccca cgtcagcccc ggtaaaggtc tggtgatcca
    15721 ccatgaatcc tgccgtaata tccgtggcta ccagaaagag ccagagaagt ttatggctgt
    15781 ggaatgggat aaagagacgg cgcaggagtt catcaccgaa atcaaggtgg agatgttcaa
    15841 tcatcagggc gcgctggcaa acctgacggc ggcaattaac accacgactt cgaatattca
    15901 aagtttgaat acggaagaga aagatggtcg cgtctacagc gcctttattc gtctgaccgc
    15961 tcgtgaccgt gtgcatctgg cgaatatcat gcgcaaaatc cgcgtgatgc cagacgtgat
    16021 taaagtcacc cgaaaccgaa attaatgttt tatgaaccca acacgttatg cacgcatctg
    16081 cgaaatgctc gccaggcggc agcctgatct gaccgtctgc atggagcagg tccacaaacc
    16141 tcataacgtt tctgcgatta ttcgtaccgc agatgccgtt ggcgtacatg aagttcacgc
    16201 cgtctggcct ggtagccgca tgcgcaccat ggcttcggca gcggcgggta gtaacagctg
    16261 ggtacaggtg aaaacacacc gcaccattgg cgatgccgtc gctcatctca aaggccaggg
    16321 catgcagatt ctggcaaccc atctttctga taatgctgtc gatttccgcg aaattgatta
    16381 cactcgcccg acctgcattt tgatgggaca ggagaaaacg ggcatcacgc aggaagcatt
    16441 ggccctggcg gatcaggaca tcatcattcc gatgatcggc atggtgcagt cgctgaatgt
    16501 ttccgttgcc tcagccctca ttctttacga agcccagcgt cagcggcaaa atgcaggcat
    16561 gtacctgcgt gaaaacagca tgttgccgga agcagagcaa caacgcctgt tgtttgaagg
    16621 cggctatccg gtgctggcga aagtcgcaaa acgcaaaggc ctgccttatc cccacgtcaa
    16681 tcagcaaggc gagatcgaag ctgatgccga ctggtgggct actatgcagg ctgcagggta
    16741 agtgccatga aaggtcgcct gttagatgct gtcccactca gttccctaac gggcgttggc
    16801 gcagcactta gtaacaaact ggcgaaaatc aacctgcata ccgtgcagga tctactctta
    16861 caccttcccc tgcgctacga agatcgcacc catctctacc ccatcggaga actactgccg
    16921 ggcgtttatg ccacggtgga aggcgaagtg ctgaactgca atatctcctt cggcggtcgg
    16981 cggatgatga cctgccagat cagcgacggt tccggcatcc tcaccatgcg ctttttcaat
    17041 ttcagcgcgg caatgaaaaa tagcctggcg acgggccgcc gtgtactggc ttatggcgaa
    17101 gcaaagcgcg gtaaatatgg tgcggagatg atccacccgg aataccgcgt gcagggcgat
    17161 ctcagcacgc cagaattaca ggaaacgctc acgccggttt atccaacaac ggaaggcgta
    17221 aagcaggcca cgctgcgtaa attaaccgac caggcgctgg atctgctcga cacctgcgcc
    17281 attgaagaac tcctgccgcc ggaactgtca caaggaatga tgacgctacc ggaagcgttg
    17341 cgcactttgc accgcccgcc accgacgcta cagcttagcg atctggaaac cgggcagcat
    17401 ccggcgcaac gtcgtctgat tctggaagaa ctgctggcgc acaacctcag catgttagcc
    17461 ttacgtgccg gagcacagcg ttttcatgcc cagccgctga gcgccaatga cacgctgaaa
    17521 aataaactcc tcgccgcctt accgttcaag ccaacgggcg cacaggcacg cgtagtggcg
    17581 gagatcgagc atgatatggc gctggatgtg ccgatgatgc gcctggtaca gggcgatgta
    17641 ggttccggta aaacgctggt cgccgccctc gccgcgctgc gtgcgattgc ccacggcaaa
    17701 caggtggcac tgatggcacc aaccgaatta ctcgccgagc agcacgccaa taacttccgc
    17761 aactggtttg caccgctcgg tatcgaagtg ggctggctcg ccggtaagca gaaaggcaaa
    17821 gcgcgactgt ctcagcagga agccatcgcc agcggtcagg tgcagatgat tgtcggtaca
    17881 cacgccatct tccaggaaca ggtgcagttt aacggcctgg cgctggtgat tatcgacgaa
    17941 cagcatcgtt ttggcgtgca tcagcgtctg gcattgtggg agaaaggcca gcagcagggc
    18001 ttccatccgc atcagttgat catgaccgcc acgccgatcc cccgcacgct ggcaatgacg
    18061 gcgtatgccg atctcgatac ctcggtgata gatgagctgc cgccaggccg cacgccagtg
    18121 actacggtcg ctattcctga tacccgccgt accgacatca ttgaccgcgt gcgccacgcc
    18181 tgcataactg aaggtcgtca ggcgtactgg gtttgtacgt tgattgaaga gtcagagtta
    18241 ctggaagcgc aggcggcgga agctacctgg gaagagttga aactggcgct accagaattg
    18301 aatgttggcc tggtacacgg gcggatgaaa ccagccgaga aacaggcggt gatggcgtcg
    18361 tttaaacaag gtgagctaca tctgctggtt gccactaccg ttattgaagt cggtgttgat
    18421 gtgcctaacg ccagcctgat gattatcgaa aaccctgagc gtctgggtct ggcgcagtta
    18481 caccagctgc gcgggcgcgt aggtcgtggc gcggtggctt ctcactgcgt gctgctctac
    18541 aaaacgccgc tttctaaaac ggcgcaaatt cgcctgcaag tgctgcgcga cagtaacgac
    18601 ggttttgtga ttgcgcaaaa agatctggag attcgcggcc ctggcgaatt gttaggcacg
    18661 cgtcagacgg gtaatgctga atttaaagtg gcggatttac tgcgcgatca ggcgatgatc
    18721 ccggaagttc agcgcctggc gcgacatatt cacgaacgtt acccacaaca ggcaaaagcc
    18781 ttgatagaac gctggatgcc ggagacagaa cgttactcga atgcgtaaaa ggcggcggtg
    18841 agaagaccgc cgcttcatcg gttaaccggc aaaaatcggc aacattaaat acagcttaat
    18901 caccagcgca ttgacgatat cgataaagaa cgcaccgacc atcggcacca ccaaaaacgc
    18961 catgtgcgac gggccaaagc gttcagtgat cgcctgcatg ttggcgattg ccgttggcgt
    19021 tgcaccgagg ccaaaaccac agtgacccgc agccagcact gccgcatcgt agtttttgcc
    19081 catcatgcgc caggtaacga agatggcata caacgccatg aagatggtct gtaccaccag
    19141 aatcgccagc atcggcagcg ccagcgaagc cagctcccac agtttcagcc ccatcaacgc
    19201 catcgccagg aacaacgaca agcttacgtt acccagcacg gataccgcac gctcaaagac
    19261 gcggtaaaag cccattattg acagaccgtt gctcagaatc acgccaacaa acagtacaca
    19321 gacgaaggtc ggcagttcaa aagcagtgcc agccaaaagt tgcgcaacaa ttttccccac
    19381 cgtcaggcag atagcaatca gcgcgatagt ttcaatcagc accaacgagg tgatcatgcg
    19441 tcccacatcc ggcttttcaa acgccgtcgg gacttcctgg tcatccggaa taccgttcgg
    19501 cgtggtggag tgtttcacca gatagcgcgc caccggaccg ccaatcaagc cgcccagcac
    19561 cagaccgaac gttgcacagg ccatcgccac ttccgtcgca ttggtgaagc cataacgttc
    19621 aatgaacaat ttactccacg cagcgcccgt accgtgaccg ccggaaagcg taatagaacc
    19681 ggccaacagc cccatcagcg gatcaagccc taacaggcta gccataccaa tgccaatggc
    19741 attttgcatc accaacagac caacaaccac aatcaagaag atgccaacca cacgcccacc
    19801 ggcacgcaaa ctggcaatgt tggcgttcag gccaatggtg gcgaagaaag ccagcattaa
    19861 cggatcgcgc agggacatat caaagttgac ttcccagccc atgctttttt tcagtactag
    19921 tagcgccagc gccaccaaca aaccacccgc aacaggttcc ggtatggtgt atttcttcaa
    19981 aaaggagacg gaatggacca acttacgccc gagcagcaac gtcagcgttg cggcaacaag
    20041 cgttgctaaa gtatcgagat gaaacatagt tactcctttg tatccgcatc actctttcat
    20101 actcgtcata cttcatcgcg cagtggcaac ttcatgaact atgacgggta tcgagttatg
    20161 ctttcctggg tcaaattctc agctgttaat gaacaaatgg catggatttt aagcagaaaa
    20221 ataccaaaag ttatataaaa aagtgcattt atctcattta aatatttaga tgaggatgtg
    20281 gcaaacgttt gcttttctct ttcggtcagc taaaatgccc gctttgctac cacgggattg
    20341 ttttcgatgt ctgtttccac cctcgagtca gaaaatgcgc aaccggttgc tcagactcaa
    20401 aacagcgaac tgatttaccg tcttgaagat cgtccgccgc ttcctcaaac cctgtttgcc
    20461 gcctgtcagc atctgctggc gatgttcgtt gcggtgatca cgccagcgct attaatctgc
    20521 caggcgctgg gtttaccggc acaagacacg caacacatta ttagtatgtc gctgtttgcc
    20581 tccggtgtgg catcgattat tcaaattaag gcctggggtc cggttggctc cgggctgttg
    20641 tctattcagg gcaccagctt caactttgtt gccccgctga ttatgggcgg taccgcgctg
    20701 aaaaccggtg gtgctgatgt tcctaccatg atggcggctt tgttcggcac gttgatgctg
    20761 gcaagttgca ccgagatggt gatctcccgc gttctgcatc tggcgcgccg cattattacg
    20821 ccgctggttt ctggcgttgt ggtgatgatt atcggcctgt cgctaattca ggtggggctg
    20881 acctccattg gcggcggtta cgcagccatg agcgataaca ccttcggcgc accgaaaaat
    20941 ctgctgctgg caggcgtggt tttagcctta attatcctgc ttaaccgtca acgtaaccct
    21001 tacttacgcg tggcctcact ggtaattgcg atggcggccg gatatgcgct ggcgtggttt
    21061 atgggcatgt tgccagaaag caacgaaccg atgacgcaag aactgattat ggtgccaacg
    21121 ccgctctatt acggtcttgg cattgaatgg agtctgctgc tgccgctgat gctggtcttt
    21181 atgatcactt cgctggaaac cattggcgat atcacggcga cctctgacgt ttccgaacag
    21241 ccagtgtccg gtccgctgta catgaaacgc ctgaaaggcg gcgtgctggc aaacggcctg
    21301 aactcgtttg tttcggcggt gtttaacacc ttcccgaact cctgcttcgg gcaaaacaac
    21361 ggagtgatcc agttgactgg tgttgccagc cgctatgtcg gttttgtcgt cgcgctgatg
    21421 ttgatcgtgc tgggtctgtt cccggcagtg agcggttttg tacaacacat tccagaaccg
    21481 gttctgggcg gcgcaacgct tgtaatgttt ggcaccatcg ccgcctccgg tgtgcgtatc
    21541 gtttctcgtg agccgctgaa ccgtcgggcg attctgatta tcgcgctgtc gctggcggtt
    21601 ggtctgggcg tgtctcagca gccgctgatt ttgcagtttg cccctgaatg gctgaaaaac
    21661 ctgctctcct ccgggatcgc cgcgggcggt attactgcca tcgtgctgaa tctgattttc
    21721 ccaccagaaa aacagtaatt cactggcggc ggtaagataa ttaccgccgc agtctcacct
    21781 ctttcaccat tcccgccttg aggattgcgc gtaaatcgtg cataactccc ttatgtgcat
    21841 ttcacgggat ggaagaccat gaaatttatt gggaagctgc ttctctacat tctcatcgct
    21901 ctgttagtgg tgatcgctgg cctctatttt cttctgcaaa cccgctgggg agcagaacat
    21961 atcagcgcat gggtttccga gaatagcgac tatcatctgg ccttcggggc gatggatcac
    22021 cgtttttccg cgccatctca tatcgtgctg gagaatgtca cgtttggtcg tgatggtcag
    22081 cccgcgcccc tggtggcaaa aagtgtcgac attgcgctaa gcagtcgcca actgaccgaa
    22141 ccacgccatg tcgataccat cctgctggaa aacgggacgc tgaatctcac cgaccagacc
    22201 gcgccgctac cgttcaaagc cgatcgtctg caactgcgtg atatggcgtt taatagcccg
    22261 aatagcgaat ggaaactgag cgcgcagcgg gtaaatggcg gcgtggttcc gtggtcacca
    22321 gaagccggta aagtgctggg tacgaaggcg cagattcagt ttagtgccgg atcgctttcg
    22381 ctcaatgatg ttcctgccac caatgtactg attgaaggca gtattgataa cgatcgcgtt
    22441 acgctgacta acctgggtgc cgacatcgcc cgcgggacat taaccggaaa cgcgcagcgt
    22501 aacgccgacg gcagctggca agtggaaaat ctgcgcatgg cggatatccg tctacaaagc
    22561 gaaaaatcgc taaccgactt ctttgcgcca ttacgctctg tcccgtcgtt gcagattggt
    22621 cgcttggaag tcatcgatgc tcgtctgcaa ggtccggact gggcggtgac cgacctcgat
    22681 ctcagcttgc gcaacatgac cttcagtaaa gatgactggc agacacagga aggcaaactg
    22741 tcgatgaacg ccagcgagtt catttatggt tcgctgcatt tatttgaccc gattataaac
    22801 gcggaatttt ccccgcaggg cgtagcgctg cgccagttca ccagccgctg ggaagggggt
    22861 atggtcagaa cgtcagggaa ctggctgcgt gacggtaaaa cgttgatcct tgatgatgcg
    22921 gcaattgccg ggctggaata taccttgccg aaaaactggc aacagttgtg gatggaaacg
    22981 acacccggtt ggttaaacag cctgcaactg aagagattta gcgccagccg caatctgatc
    23041 attgatatcg accctgactt cccgtggcag ctcaccgcgc tcgatggtta cggtgccaac
    23101 ctgacgctgg ttaccgatca taaatggggc gtctggagtg gctcggcgaa tctgaatgcc
    23161 gccgccgcga cattcaatcg tgttgatgtt cgtcgcccgt cgctggcgct gaccgccaac
    23221 agcagcacgg tgaatatcag cgaactgagt gcatttactg aaaaaggcat tctggaagcc
    23281 accgcaagtg tttcacaaac gccacaacgt cagacccata tcagcctgaa tggacgcggt
    23341 gtgccggtga atattttgca acaatgggga tggcctgaat taccgttgac tggcgacggc
    23401 aatattcagc ttaccgccag tggagatatt caggccaatg ccccgctgaa acctacggtt
    23461 agcgggcaat tgcatgccgt gaacgccgca aagcagcaag tgactcaaac catgaatgcg
    23521 ggcgtcgttt ccagcggtga agttacatcg acggagccgg tgcggtaaac atcaatctcg
    23581 ccgggtacga ctcagacgta cccggcattc cgtcaataga ttacaacgta attgtcagcg
    23641 cattcccttg aggcgtcacc accagcccct gctcactttc agcctgcgaa ccgccttgca
    23701 gaccatttac tttcacaaca ttgcgcaagc acagcgtcca gttcttcgcc tcgcccgcac
    23761 cagtcacagt aatcgtgttg ccagtacgtg ctgcttttaa agtaaagatc accgatccgt
    23821 cagcagcagg cacttcacag acggcttcat gcccgtcttg cagattaaag aggtggaatg
    23881 ccgtgccttc gtgccacacg taatcgggac gttgatcgtt gttgcccagc gccagtagag
    23941 tgttatcacg cacataaacg ggcagactca ggaagccgtg ctgctgttta tgccagcgac
    24001 taccgtcgag ttcatcgttg tgccacaggt gtgtccagcg accttccggc aggtagaact
    24061 gcacatcgcc cgcttcagtg aacaccggcg caaccatcac gttgtcgcct aacatgtatt
    24121 gacggtcaag gtaatcacaa gccggatcgt ccgggaactc catcatcatg gcccgcatca
    24181 tcggcgtacc ccgcgcgttc gcacgcgcag cttcacgata cagatacggc atcatgcggc
    24241 atttcagttg cgtgaagaag cgcaccacat cacaggactc atcatcgtag gcccacggca
    24301 cacgataaga tttgctaccg tgtaaacggc tatggctgga gagcaaacca aacgcgcacc
    24361 agcgtttgta aacgtgcgcc ggagcggtat tttcaaagcc gccgatatcg tggctccaga
    24421 agccaaaacc tgaaaggcca atagacaaac caccgcgcag gctttccgcc attgattcgt
    24481 agttagcgta acaatcgcca ccccagtgta ccgggaattt ctgcgcaccg acggaggccg
    24541 agcgggcaaa caagacagct tcttcctcac caacggtgtc cttgagcacg ttccacacca
    24601 gttcgttgta gatgtacgca taatggttat gcattttctg cggatcggaa ccgtcaaacc
    24661 actgaacatc aattgggata cgttcgccaa agtcggtctt aaagcaatca acgcccatcg
    24721 cgaccagacc tttcagtttg tcggcgtacc atttgcaggc atccggattg gtaaagtcat
    24781 aaatcgccag acctggctgc catttatccc actgccatag cgaaccgtcc gggcgtttga
    24841 gtaaatagcc tttctcttgt aactctttaa agacggggga tttttgaccg atataggggt
    24901 taatccagac gcagattttc agccctttcg ctttcaggcg gcggatcatc ccttccgggt
    24961 cagggaaagt cagcgggtcc cactcaaaat cgcaccactg gaaggctttc atccagaaac
    25021 agtcaaagtg gaaaacatgc agcggcagat tgcgttccgc cataccatcg ataaagctgt
    25081 ttaccgtcgc ttcgtcgtag ttggtggtaa atgaagtggt tagccacagg ccgaaggacc
    25141 acgggggcgg cagcgccgga cgaccagtaa agcgggtata acgatcaagt accgctttcg
    25201 gcgtcgggcc gtcgataaca aagtattcga gatattcact ctcaacgctg aactgcactt
    25261 tggagacttt ctccgatccc acttcaaaag agacacactg gggatgattg accagtaccc
    25321 cataaccacg gttagtcatg tagaacggga tatttttata cgcctgttca gtacttgtgc
    25381 cgccgtcccg gttccaggtc tctaccgtct ggccattgcg caccagggca gtaaagcgct
    25441 ctcccagacc gtaaactgtt tcgccaacgc caagatcaag ccgctcaaac atataattgc
    25501 gttgattatt cgtgtcctgc acgtagccat tatttttcac ctgactaccg gtaatacgtt
    25561 cgccgttgcg cagaaaatcc agtgaccaga actcaccttt gctgacacgc gcgcttaagt
    25621 tgccactttt aaactcagca taacgttctg tgttttcgat tgtgaccttc acgtcctgca
    25681 aaatattgag cggataatga ggaccgttat tcagcgcccc ctgaaaatgc tcaatccgca
    25741 caccgacaat accttcctgt ggggagaaaa agcgcagcgt aaataaaggc gtatcaagct
    25801 gccaggtacg ttcacgcaca tcacgggggg cagcatagac caccatttca ttatcctgct
    25861 gttcaacctc gaatacctga agcgggtgaa tcaaattgag gccaggttga atcaaccagt
    25921 ttccatcgct aattttcatt gccgttcctt agttctgcac ttcttgagag gtgaattgtt
    25981 gctggcaacg gcgtttaccc tgagccagct gttccataac ggttttcaga ttgtgcgtcg
    26041 tgagtgagta gtagcgttta gcgataatcg cgctcagcaa ataacagatc gccggaacaa
    26101 tcgtgaatag cgcaataatg atgctaatcg tggcgctgtt ctgcgctttt tctgccgcat
    26161 catatccgcc ataagccagc atccagccga taagagcgcc gccgaaggcc aaccccagtt
    26221 tgagcacaaa cagcgtgcca gcaaaactga tcccggtcag gcgtttacca ttgcaccact
    26281 cgccgtagtc gacggtatcg aacatcatta cccactggat aggtgtcacc agttgatgca
    26341 acacaccaat cacgaagatg aagacaaaca tagtgatgct ggcctgcatg ggaacaaaga
    26401 acatcgcgag gctaatcact gccagcaggg cgttcgtcca ccagaagata gtgactttac
    26461 atttccagtc ggtcagaggt tttgccagtg cggaaccaat caggttaccc acgcaataag
    26521 tggtgagaaa agcgacaaac acttccggcg tgcccaaaat ccatgtgacg taatacatca
    26581 tcgccccacc gcgtacgcac accgccagga tattgaaaat ggttagtaaa ccgacaatcc
    26641 gccactggtc gttttgccag atatcacgta aatcttcccg catagacgtt gttgtagatg
    26701 gtgcttcaac gcgttcttta gtggtgaaga aacaaaatgc cagcatcatg aatgccacca
    26761 cggaaaggac cacgataccg ccctggaaac cgagtggttt attatcaccg ccaattaaat
    26821 taaccagtgg catcatcaga acagtagaaa gcatgcctcc cgcggtcgcc agcacaaaac
    26881 gccaggattg cagcgagata cgctgagtcg ggtcattggt gattacacca cccaatgcgc
    26941 agtaagggat attgacgacg gtataaagta aggtaagtta gggtgtaagt aattgctgca
    27001 tagatcattt tgccgttcat actgagatct ggcgtgctat aggccagtac acagacgatc
    27061 ccgaatggca gtgcgccaaa cagtacccac ggacgaaatt taccccagcg agagcgcgtt
    27121 cgatcggcca acaaccccat gcaaggatcg gaaatcgcat ccagtgcacg agcgaccaaa
    27181 aacatggttc cgacaaatcc ggcaggaatg ccaaaaatat cggtataaaa gaacatcata
    27241 tataacatta cgttatcgaa aataatgtgg ctggcggcgt ctcccatgcc ataaccaatt
    27301 ttctctttaa cggacaacac ttcactcctc atctgctttt ccttgatgct ataggggtgc
    27361 tgagaccgat aacgcatttt gtaaaccatc cctgtgagag cgaatattcc gatatctggt
    27421 tattaattta tgattcttgt tttatgtgat cgtggtagcg ttaattccgc tcatatatca
    27481 ttgtaaaata tgggttttat ataaattata atgctttcgt gataatacgc tgcgtttatt
    27541 aggcggaaaa aactgatctg ggggatgtag aaactcaagg aagtagctat aatgcgcccc
    27601 gcctccatgt agcaatcgag gcgcggaaga tcgtcgtctc cggtgaggcg gctggacttc
    27661 aaatccagtt ggggccgcca gcggtcccgg gcaggttcga ctcctgtgat cttccgccag
    27721 tttacctctt ctgaactctc ccaatgtcaa taaatctctt ctaatccctt gtatatcagt
    27781 atttatttgt atcctgaggt caacctactt caccccgtat caagtttctt ttgggggtac
    27841 tttagggggt gttatttcct attgaaatac aagcaccccc ataataggaa aaatgtgata
    27901 cataacaacg gcttttaaca atatctttgt tatacaaggg gtgtattggg ggtatcaggt
    27961 tagttactgg cagaaatccc cctcaggaac atgaattcat caaaaagccg gaggatgtca
    28021 tggcattaac cgatatcaaa gtgaaaaccg caaaacccaa agacaaaccc tataagcttg
    28081 ctgatggcgg cggtatgtat ctactgatta acactaatgg ttctaaatac tggcgcatga
    28141 agtaccgctt tgctggtaaa gaaaagatgc tttctattgg cgtatacccc gatgtaacgc
    28201 tagccgatgc tcgcgagaaa cgcagtgaag ccaggaaact cctcgctgct ggaggcgatc
    28261 caagggaggc aaaaaaggaa gaaaaaatcg cgcagcaaat gagtctgaaa aatacgttcg
    28321 aggctattgc tcgcgaatgg caccagttaa aagctgatcg gtggtcatta cgctatcgtg
    28381 atgaaatcat tgataccttt gaaaaagaca tttttcccta cattggcaag cgccctattg
    28441 ccgaaatcaa accaatgaag ttactggaag cactgcgtaa aatggaaaaa cgtggagctc
    28501 tggagaaaat gcgcaaggtc cgccagcgct gtggtgaggt gttccgctat gcaattgtta
    28561 ctggccgggc agactataac ccagcccccg atcttgccag tgctttagct acaccgaaaa
    28621 aagtacattt tccctttctt actgctaatg aacttcccca cttcctcaac gatctggcgg
    28681 gttataccgg aagtataatc actaagacag ctactcagat cattatgtta accggcgtac
    28741 gaacacaaga actgcgtttt gcacgctggg aagatatcga ttttgagaca aaattgtggg
    28801 agatcccggc agaagtaatg aagatgaagc gacctcatat tgtgccgctc tctgagcagg
    28861 tcattatgct atttaagcag cttgaaccaa tctcaaaaca tcatccccta gtctttatcg
    28921 gtagaaatga tccccgcaaa ccaattagta aggaaagcat taaccaagta attgaattac
    28981 ttggatataa gggaagactc acagggcacg gtttcagaca caccatgagt acaattctgc
    29041 atgaacaagg ctttaattcc gcctggattg aaatgcagtt agctcatgtg gataaaaatt
    29101 ccatcagggg tacctataat catgccctgt atctcgatgg ccgccgtgaa atgatgcaat
    29161 ggtacgctga ttacattgat tcactctcca gccgggaaag ttaagtcaga aatacttttc
    29221 cctgatgttt tcctcaatta agtattttat cctctggcct acaaggctgg aggatttttg
    29281 ttattatatt atttttttgc gtcttcaaac ttattacaaa aacataatta aaaacaaatt
    29341 gacattttta aattggcaat tacaaaatac agccttgcag ttatttcgat acctttagca
    29401 aaggaatagt tgattcagat ataatttagc atttcaatca tctgacatca ttataaaaaa
    29461 tgagattcac ataaagataa aattattgtt ctcaaggaaa gcatgaaggg gcttcgtaag
    29521 caatattcat tcggtgccaa gacaggactt gatacacaga atctcaactt ttatgctctc
    29581 agactatatc accacatgtt ttacaggctc agaaaatgaa cgatagatta tgctttgaag
    29641 tgcacgataa caagggttat ttcgtatttc cggatacctg gtttggccca ttgctggggg
    29701 agtttgaaga agtgcttgat gcatacgacg ctgatgaaat cagcgagacg agttacatca
    29761 acaaactccg acgccttgct caacgggagc cggactttat tgatatacac gctcatctgg
    29821 catatgcatt tctggaacag aatgccccgc gtaaggcact gaacgccgct cttaaaggac
    29881 tggcggcagg taatcgtatc atcccggaaa gtttctgtgg tgaaataatc tggatgcatc
    29941 ccgaaaaccg gccttatctg cgcgcgcttt atgcagcaat tctcgcaaat gtgcacttac
    30001 agcgccatca ggatgcggtt atgctcactg ataaaatact ggcatacaat cctgaaaata
    30061 atcagggggc tcgctggttg ttgggttcag agctgttacg caccggcgat catgagcgag
    30121 ccttcagtgt actgaaagag catgctgatg aattttcacc ttactggtat gaactcggtc
    30181 tcctgcattt tctgaacggg gaacatgtta aggccgccac agcttttcgc catggctttg
    30241 ccacaaacac ttacatcgca gaaatgctgt gcggaaatct gcatccattt ccacttgctg
    30301 tgtggcatga cttctccggc tctctggata cggcagaaga ttactatgca acatactcac
    30361 cattatgggg acaatatccg gaagcattgc tgtttgtaaa ctggctctat aaccattcct
    30421 ccgtgctgca tgaacgatca gaaatcatta aatgcgccga aatgctgatt caggaagatg
    30481 atttcgaaat atgcgaaagt attttaagac agcaggagca cctgtggaaa agaattgaca
    30541 agacactttc tgaggaaatt gtacagaaat gcagaaatat gaacggtgaa tatatctggc
    30601 cctggatatt gcccttttcc gcagcaggga tgaaacattc aagcatccag tatcagtaga
    30661 tattgcatta gtgtaacttt aaaacaagta ataatttatt atccaataat aacactgtgt
    30721 tccttcccca ggtgattacc ctggggttat tttctgaaag tttcctttgc aaaaggatag
    30781 cgttgtaaat tactttaacg aattactcca tgaactcatt aaagttcttt cgtaatactt
    30841 tatcgtcaaa cacgttatag ctggcatcaa aaaccacaga tttaccctgc cagcgggtaa
    30901 tcccctcctc tggcgttttc ggttgaactg gtatccccgt ccaacacagg cagccattat
    30961 aaacagtaaa aaattgtatt cgctgttatg aaatcaatag tcctgacgta ctgttgaatg
    31021 tgcttgtgtt ctgaacctcg tacaaccagt acatttgata tcgaattata aacaaaacat
    31081 tgcatccaac ttttttaata tggacaatag ttgtccatac ggtggtttta tattcgataa
    31141 atcaatggat ttcattgagt cacaggactg gtaatggatg aaaatgcttt aaggtttgcc
    31201 tcatactggc gcaactcgct tgcagatgct gagtcaggaa agggctgttt tgaacggaaa
    31261 gacgcccaaa atttcactca ctggcatggg atagcggcgg gacgtcttga cgaagcgatt
    31321 gtcaataaat tttttaaggg agaaaaagac gatgtcgaaa ctgtcaatgt catcttacgc
    31381 ccaaaagttt atttccggtt actgcagcat ggtaaggacc gttccgcagg cacgcctgat
    31441 attgttacac cgatagtgac gccagccttg ctaagccgtg agggtttttt atatccgacg
    31501 ccagcgacct ccattcccag agacctgctt gaacctttgc caaaaggagc attttcgatt
    31561 ggtgagattg ggcagtatga caaatacaag acgacccata ctgatcctac ccacgtaatg
    31621 tggacacggc cctaagcgag attatggttt tcaaattgtt ccgggctgag accgccacag
    31681 gcactgtgac gacgccagcg attgtaatca cactcgatat aattaaacac cgttgcccgc
    31741 attatttccc ggctgctaaa gcgttccccg tggatacatt ccaccttcag cgaatgaaag
    31801 aagctttcca cacaggcatt gtcataacag tagcctttcg cactcatact gccacgcagg
    31861 ttgtgtcgct tcagcattgc ctgataatcc cctgaacagt attgaccacc acggtccgta
    31921 tgaacaatga cgctttccgg gcgtcttctc cgccacaacg ccatttgcag tgcatcacag
    31981 gccagttgtg ctgtcattcg cggtgacatc gaccagccaa tgacggagcg tgaccacagg
    32041 tcgatgacta ctgcgagata caaccagccc tcatcggtac gcaagtacgt gatgtcaccc
    32101 gcccacttct ggtttcgggc cactggcgct gaagtcctgc tccagcagat tctccaatac
    32161 gggcaggcca tgtgcacggt agctgaccgg gctgaacttc cggctggctt tcgcccgcag
    32221 cccctgacga cgcaggctgg cggcaatggt tttaatattg aactccggca gttcgtcagc
    32281 aaggcgggga gcaccgtatc gctgctttgc ctcaaagaat gccttatgga cagcggcatc
    32341 gcaggtgagc cgaaactgtt ggcgcaggct catctggtga cgacgcctga gccagacata
    32401 ccagccgctg cgggcaactc gaagtacacg acacatcgct ttgatgctga actctgcccg
    32461 atgattttcg ataaagacat acttcatttc aggcgcttcg cgaagtatgt cgcggccttt
    32521 tggaggatgg acagttcctc agcctgctcc gccagttgtc gtttaaggcg gacattttca
    32581 gcggccagtt cgctttcgcg ctctgacgaa ctcatttgtt gctgctgttt actgcgccag
    32641 gcataaagct gagattcata caggctgagt tcacgggctg cggcggccac accgatgcgt
    32701 tcagcgagtt tcagggcttc gttacgaaat tcaggcgtat gttgtttacg gggcttcttg
    32761 ctgattgata ctggttttgt catgagtcac ctctggttga gagtttactc acttagtcct
    32821 gtgtccacta ttggtgggta agatcaccct ccattaaaac taaaacgggc gctttctgat
    32881 atatcatctg taagcgcagt aataccatta gtagcacctc tcggcggcgc attgattatt
    32941 gaatattata actggaatgt tatatttctt gttatgatgc tgttcagtat tataataatg
    33001 ttattatcac caaagataac tcatgcaaat gagaaagtaa tatgtcaaaa cagtgcatcc
    33061 tttgtgacca aagagtttat ctgcggcact atacttgtct cattaatact cagttctcta
    33121 ttttgctaca tatcaatatc accatcaatt ttcatgcata cttatggcct gagtactttt
    33181 agttactcaa ttattttctc cttctcagtt ttatttttca taatcggaaa tcaattatca
    33241 aagattgaaa aattatccaa cctatggttt ttattaccca atagccaatt ttatcgcatt
    33301 acaaataaat ggttcaaaaa caggatttgc cgcatctatt acaggtttta ctcaaacaat
    33361 aagcgctggt acaatcagtt tcctgtcagt aaccgtaagc gacaacggtg ttggtcccgg
    33421 aactacttta ggtcttggtt cactaataac ttcattactg tcaatagccg taatcgccat
    33481 tgcaggaaat aataaaaagt aagtgtactc tgactgcgta tgtatctact tgaaccgccc
    33541 cgg
//
LOCUS       DAJTQY010000036        33124 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.036, whole genome shotgun sequence.
ACCESSION   DAJTQY010000036 DAJTQY010000000
VERSION     DAJTQY010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33124
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.036"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(2..94)
                     /locus_tag="OO349_001801"
     tRNA            complement(2..94)
                     /locus_tag="OO349_001801"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Ser,seq:gct)
     gene            complement(410..595)
                     /gene="csrA"
                     /locus_tag="OO349_001802"
     CDS             complement(410..595)
                     /gene="csrA"
                     /locus_tag="OO349_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HCR5866903.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(830..3460)
                     /gene="alaS"
                     /locus_tag="OO349_001803"
     CDS             complement(830..3460)
                     /gene="alaS"
                     /locus_tag="OO349_001803"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HCR5866904.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADIDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMNLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3588..4088)
                     /gene="recX"
                     /locus_tag="OO349_001804"
     CDS             complement(3588..4088)
                     /gene="recX"
                     /locus_tag="OO349_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HCR5866905.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4331..5392)
                     /gene="recA"
                     /locus_tag="OO349_001805"
     CDS             complement(4331..5392)
                     /gene="recA"
                     /locus_tag="OO349_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HCR5866906.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5472..5969)
                     /gene="pncC"
                     /locus_tag="OO349_001806"
     CDS             complement(5472..5969)
                     /gene="pncC"
                     /locus_tag="OO349_001806"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HCR5866907.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(6114..7199)
                     /gene="mltB"
                     /locus_tag="OO349_001807"
     CDS             complement(6114..7199)
                     /gene="mltB"
                     /locus_tag="OO349_001807"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HCR5866908.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAIMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            7455..8017
                     /gene="srlA"
                     /locus_tag="OO349_001808"
                     /gene_synonym="gutA"
                     /pseudo
     CDS             7455..8017
                     /gene="srlA"
                     /locus_tag="OO349_001808"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
     gene            8014..8973
                     /gene="srlE"
                     /locus_tag="OO349_001809"
                     /gene_synonym="gutE"
     CDS             8014..8973
                     /gene="srlE"
                     /locus_tag="OO349_001809"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HCR5866909.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSSFIYQ"
     gene            8984..9355
                     /gene="srlB"
                     /locus_tag="OO349_001810"
                     /gene_synonym="gutB"
     CDS             8984..9355
                     /gene="srlB"
                     /locus_tag="OO349_001810"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HCR5866910.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            9359..10138
                     /gene="srlD"
                     /locus_tag="OO349_001811"
     CDS             9359..10138
                     /gene="srlD"
                     /locus_tag="OO349_001811"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HCR5866911.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSRMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            10243..10602
                     /gene="gutM"
                     /locus_tag="OO349_001812"
                     /pseudo
     CDS             10243..10602
                     /gene="gutM"
                     /locus_tag="OO349_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
     gene            10669..11442
                     /gene="srlR"
                     /locus_tag="OO349_001813"
                     /gene_synonym="gutR"
     CDS             10669..11442
                     /gene="srlR"
                     /locus_tag="OO349_001813"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HCR5866912.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            11435..12400
                     /gene="gutQ"
                     /locus_tag="OO349_001814"
     CDS             11435..12400
                     /gene="gutQ"
                     /locus_tag="OO349_001814"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HCR5866913.1"
                     /translation="MSEALLNTGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEVLMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(12397..13911)
                     /gene="norR"
                     /locus_tag="OO349_001815"
     CDS             complement(12397..13911)
                     /gene="norR"
                     /locus_tag="OO349_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HCR5866914.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFVGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRQGLSRVVLSAGAR
                     NLLQHYSFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVL
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            14098..15537
                     /gene="norV"
                     /locus_tag="OO349_001816"
     CDS             14098..15537
                     /gene="norV"
                     /locus_tag="OO349_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HCR5866915.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAMDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            15534..16667
                     /gene="norW"
                     /locus_tag="OO349_001817"
     CDS             15534..16667
                     /gene="norW"
                     /locus_tag="OO349_001817"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HCR5866916.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSIDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAGQFNLRLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSGMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFVLLKTLPV"
     gene            complement(16795..19047)
                     /gene="hypF"
                     /locus_tag="OO349_001818"
     CDS             complement(16795..19047)
                     /gene="hypF"
                     /locus_tag="OO349_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HCR5866917.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSTGGTMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRERKHRPAKPLAVMLPVAESLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLLHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALVLPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYIV
                     HDVHPGYVSSQWASEMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCDGVTHPVTMPRVDNQLDLATFWQQWLNWQALVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(19200..19727)
                     /gene="hydN"
                     /locus_tag="OO349_001819"
     CDS             complement(19200..19727)
                     /gene="hydN"
                     /locus_tag="OO349_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HCR5866918.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVPVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(19876..20886)
                     /gene="ascG"
                     /locus_tag="OO349_001820"
     CDS             complement(19876..20886)
                     /gene="ascG"
                     /locus_tag="OO349_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HCR5866919.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESS
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRNNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALSQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAIKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            complement(20900..21019)
                     /locus_tag="OO349_001821"
     CDS             complement(20900..21019)
                     /locus_tag="OO349_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur protein"
                     /protein_id="HCR5866920.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAQGQSANGAVHCVDCV"
     gene            complement(21029..22738)
                     /gene="hycE"
                     /locus_tag="OO349_001822"
     CDS             complement(21029..22738)
                     /gene="hycE"
                     /locus_tag="OO349_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HCR5866921.1"
                     /translation="MSEEKLGQHYLAALNEAFLGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHPHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGLARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(22756..23679)
                     /gene="hycD"
                     /locus_tag="OO349_001823"
     CDS             complement(22756..23679)
                     /gene="hycD"
                     /locus_tag="OO349_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HCR5866922.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWMFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(23682..25475)
                     /gene="hycC"
                     /locus_tag="OO349_001824"
     CDS             complement(23682..25475)
                     /gene="hycC"
                     /locus_tag="OO349_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005063859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HCR5866923.1"
                     /translation="MSPPLFWHFSFQKALSGWIAGIGGAVGSLYTAAAGFTVLTGAVG
                     VSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHAQVKCNGLQINMLMAA
                     AVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALGRLGTLLLVIACWLLWQR
                     YGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWVPQAHANASAPAAALF
                     STVVMKIGLLGMLTLSLLGGNAPLWWGIALLVLGMITAFVGGLYALMEHNIQRLLAYH
                     TLENIGIILLGLGLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSLFKSVLFLGAGS
                     VWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEWVIYQSFFKLSN
                     SGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAENATCAPLLMSVS
                     VVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLIACPLLPFIIMA
                     ICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKLRKWLNPVSLVP
                     GWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(25512..26123)
                     /gene="hycB"
                     /locus_tag="OO349_001825"
     CDS             complement(25512..26123)
                     /gene="hycB"
                     /locus_tag="OO349_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HCR5866924.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(26248..26709)
                     /gene="hycA"
                     /locus_tag="OO349_001826"
     CDS             complement(26248..26709)
                     /gene="hycA"
                     /locus_tag="OO349_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HCR5866925.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLYH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            26921..27271
                     /gene="hypA"
                     /locus_tag="OO349_001827"
     CDS             26921..27271
                     /gene="hypA"
                     /locus_tag="OO349_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCR5866926.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            27275..28147
                     /gene="hypB"
                     /locus_tag="OO349_001828"
     CDS             27275..28147
                     /gene="hypB"
                     /locus_tag="OO349_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HCR5866927.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPQMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHTPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            28138..28410
                     /gene="hypC"
                     /locus_tag="OO349_001829"
     CDS             28138..28410
                     /gene="hypC"
                     /locus_tag="OO349_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HCR5866928.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            28410..29531
                     /gene="hypD"
                     /locus_tag="OO349_001830"
     CDS             28410..29531
                     /gene="hypD"
                     /locus_tag="OO349_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HCR5866929.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            29528..30538
                     /gene="hypE"
                     /locus_tag="OO349_001831"
     CDS             29528..30538
                     /gene="hypE"
                     /locus_tag="OO349_001831"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HCR5866930.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAGSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            30612..32690
                     /gene="flhA"
                     /locus_tag="OO349_001832"
     CDS             30612..32690
                     /gene="flhA"
                     /locus_tag="OO349_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HCR5866931.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAGGHIFGGCEFIRYDERPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     YERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGSGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNSVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(32727..33080)
                     /locus_tag="OO349_001833"
     CDS             complement(32727..33080)
                     /locus_tag="OO349_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HCR5866932.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
BASE COUNT         7805 a         9227 c         8621 g         7471 t
ORIGIN      
        1 atggcggtga ggcggggatt cgaaccccgg atgcagcttt tgaccgcata ctcccttagc
       61 aggggagcgc cttcagcctc tcggccacct caccacacgc ctcttacgag tgctttgaag
      121 aacttgttta tcgctcatca tcgctgcgtg gcgcacatat tactttctga gacttataag
      181 tcaaacaatt tttcccacac ttttatcgtt tgcacacttc acgctcaatt agtctgtaaa
      241 agcgtcaaaa agagtgtttt atctacagaa aaacggaggt ctggcagata gcagtaatgc
      301 aaaaaaatgg agacttaagt tgaatgaacg ggagtaaagc gaaaagacta tagagtgaag
      361 gagaaatttt gagggtgcgt ctcaccgata aagatgagac gcggaaagat tagtaactgg
      421 actgctggga tttttcagcc tggatacgct ggtagatctc ttcacggtga acagaaactt
      481 ccttcggggc atttacgcca atacgtacct ggttgccctt tacccctaaa actgtcacgg
      541 tgacctcatc cccaatcatg agggtctcac caactcgacg agtcagaatc agcattcttt
      601 gctccttgaa agattaaaag agtcgggtct ctctgtatcc cggcattatc catcatataa
      661 cgccaaaaag taagcaatga caaacacatt acatgtaagc agtcatggca ttacattctg
      721 ttaaacctaa gtttagccga tatacacaac ttcaacctga ctttatcgtt gtcgatagcg
      781 ttaatgcgaa tgccgtgaag cgagtccacg gcattgcctg acgcttatat tattgcaatt
      841 tcgcgctgac ccagcctttc acactggcta acgctgcagg taaggccgca gcatccgtac
      901 caccggcttg cgccatgtca ggacgtccac cacccttgcc gcccacctgc tgagcgacca
      961 taccaatcag ttcccctgct ttcacacgat ctgtgacgtc cttagatacg cctgcaatca
     1021 gagaaacctt accttcggct accgttgcca gcacgataat tgtcgacccc agctgatttt
     1081 ttaaatcgtc aaccatggta cgcaacattt tcggctcaac accgctaagc tcgctaacca
     1141 acagcttaac accattaaca tcaattgcct tactggaaag atttgcgctc tcctgtgcgg
     1201 cagcttgttc tttaagctgt tgtagttctt tttctaactg acgcgtacgt tccagtactg
     1261 agcgcacttt atccgccaga ttattgctat cgcctttcag cagatgcgcg acttcgctta
     1321 agcgatcgct gtctgcatga acggtggtga tagcgccttc tccggttacc gcttcgatac
     1381 gacgaacgcc tgcagcagta cccgattcag agatgatgcg gaacagacca atatcaccag
     1441 tgcggctggc gtgagtaccg ccacacagct cggtggagaa atcgcccatg ctcagcacgc
     1501 gtacacgctc atcatacttc tcgccgaaca gcgccatcgc acctttcgct ttcgccgctt
     1561 cgagattcat gatgttggtt tcgatcggca aattgcgacg aatctgtgcg ttcaccaggt
     1621 cttcgaccgc acgaatctct tccggtttca tcgcttcgtt gtgtgagaag tcgaagcgca
     1681 gcaccttgtc gttaaccagt gaacctttct gcgatacatg agtccccaga acctggcgca
     1741 gcgcagcgtg catcaggtgc gttgcggagt gattcagacg aatacgggcg cgacgagcct
     1801 catcaatatc agcctgcaca gcgtcgccca ctttcagaga acccgcagca agtttaccga
     1861 tgtgaccaat cgcctggccg tatttctgcg tatcttccac cgcgaaggag aagttagcgc
     1921 ctttcagttc gcctttatca ccaacctgac cgccggattc cgcatagaat ggcgtttgat
     1981 ccagcacgac cacagcttcc tggcctgcat tgatggcatc aaccgcttta ccatcaacaa
     2041 acagcgcggt cactttgccg ttcagttcca gatggtcata gcctttaaat tcagatgcac
     2101 tatcaacacg gatcattgcg ttgtaatcgg caccaaagcc gctggcttcg cgcgcgcgac
     2161 gacgctgctc ttccattgct gcttcaaaac cagcttcgtc aactttgatg ttgcgctcac
     2221 gacaaacatc agccgtcagg tcaaccggga agccataggt gtcgtacaga cggaaagccg
     2281 tttcaccatc cagcgtatca ccagaaagtt ttgccagctc ttcatccagc aacgccagac
     2341 cgcgctccag agtacgggca aactgctctt cttcagtctt cagcacctgc tcaacctgcg
     2401 cctgctggcg tttcaggtct tcacccgcag agcccataac gtcgatcagc ggaccaacca
     2461 gtttgtagaa gaaggtttct ttcgcgccga gcatattgcc gtgacgcact gcgcgacgaa
     2521 tgatacgacg cagtacataa ccacggtttt cattggacgg cattacgcca tccgcgatca
     2581 ggaacgcaca agaacgaatg tggtcagcga ttacgcgcag cgatttattg ctcagatcgg
     2641 tcgcgccagt gactttcgct accgcctgga tcaacgtgcg gaacaggtcg atgtcatagt
     2701 tagagttaac gtgttgcagc accgcagcaa tacgctccag acccataccg gtatctacag
     2761 acggcttcgg cagcggttcc atcgtgccat ctgcctggcg gttgaactgc atgaagacga
     2821 tgttccagat ctcaatgtag cggtcgccgt cttcttccgg acttcccgga gggccacccc
     2881 aaatgtggtc gccgtgatcg tagaagattt cggtgcacgg gccgcacgga ccagtgtcac
     2941 ccatctgcca gaagttgtca gatgcgtatg gcgcaccttt gttatcgccg atgcgaataa
     3001 tacgttcgcg cgggatcccg acttcttttt cccagatttc gtaggcttcg tcgtcgcttt
     3061 catagacggt aacccacaga cgctctttcg gcagggcaaa ccatttttcg ctggtcagca
     3121 gttcccatgc aaactgaatg gcatcgtgtt tgaaatagtc gccgaagctg aagttgccca
     3181 gcatttcgaa gaaggtatgg tgacgcgcgg tgtaaccgac gttttccagg tcgttgtgtt
     3241 taccacccgc acgcacgcag cgttgggaag tggtagcgcg ggaataatta cgcttgtcga
     3301 gcccaaggaa cacatccttg aactggttca tcccggcgtt ggtaaacaac aaagttgggt
     3361 cgttatgggg taccagggag ctgctggcaa ctacctgatg tcccttacta tggaaaaagt
     3421 cgagaaacgc ctgacggatc tcagcggtgc tcttgctcat aattatcctg aaatcaagct
     3481 aacgaaatat cgccaccagc tccagcgtgt cttaaccgcc gggctggtaa ctgaaaagtg
     3541 ggaataagat aagttttctt gactgggaag taaaataccg tatgcgttca gtcggcaaaa
     3601 tttcgccaaa tatcctggat atcttccatc agatagccac gatagagcag aaaacgctgg
     3661 atcttaactt tttctgaaaa gacagttggc aaaggttcac catattttcg cgtcgcctga
     3721 tcgcgcgcca gtgcgcacca gtcgatgtca cattcacgca tcgctttttc tgtcgcttcg
     3781 cgggaaatac ctttctgatt cagttcctga cgaatacgtg caggtccata ccctttgcgg
     3841 ctacggctgg cgataaagcg cgcaacaaat cggctgtcat cgagatagcc atgttcatgg
     3901 caccaggcaa taacgcgctc gtaatcttct gccgtagcat caatctcttc tgggccattt
     3961 ttgcccataa tcggtgccgc gagtttacgt cgcagttctt gctcactgtg atcgcgcacc
     4021 gccagaatgc gtaccgcacg atccaacagg cgagcatatg ccgggcgacg ggatgttgat
     4081 tctgtcatgg catatcctta caacttaaaa aagcaaaagg gccgcagatg cgccccaggt
     4141 tttgctgata aacagattta atagtcaatg agtatgcaga atgttcccgc aggcccaatt
     4201 cgttagagga tcgaggtgag agtaattttt cacgccagtg ctggcctgtt cccctcaccc
     4261 taaccctctc cccaaagggg cgaggggacc gtccgtgcac acattatcta ttatcatcaa
     4321 taacgaacga ttaaaaatct tcgttagttt ctgctactcc ttcgctgtca tctacagaga
     4381 aatccggcgt tgagttcggg ttgctcagca gcaactcacg tactttcttc tcgatctctt
     4441 tcgcggtttc cgggttatct ttcagccagg cagttgcatt cgctttaccc tgaccgatct
     4501 tctcaccttt gtagctgtac cacgcgcctg ctttctcgat cagcttctct tttacgccca
     4561 ggtcaaccag ttcgccgtag aagttgatac cttcgccgta gaggatctga aattcagcct
     4621 gtttaaacgg tgcagcgatt ttgttcttca ccactttcac gcgggtttcg ctacccacca
     4681 cgttttcgcc ctctttcacc gcgccgatac gacggatgtc gagacgaaca gaggcgtaga
     4741 atttcagcgc gttaccaccg gtagtggttt ccgggttacc gaacatcaca ccaattttca
     4801 tacggatctg gttgatgaag atcagcagcg tgttggactg cttcaggtta cccgccagct
     4861 tacgcatcgc ctggctcatc atacgtgccg caaggcccat gtgagagtcg ccgatttcgc
     4921 cttcgatttc cgctttcggc gtcagtgccg ccacggagtc aacgacgata acgtctactg
     4981 cgccagaacg cgccagggcg tcacagattt ccagtgcctg ctcgccggta tccggctggg
     5041 agcacagcag gttatcgata tcgacgccca gtttacgtgc gtagattggg tccagcgcgt
     5101 gttcagcatc gataaacgca caggttttac cttcacgctg cgctgcggcg atcacctgca
     5161 gcgtcagcgt ggttttaccg gaagattccg gtccgtagat ttcgacgata cggcccatcg
     5221 gcagaccacc tgccccaagc gcgatatcca gtgaaagcga accggtagag atggtttcca
     5281 catccatgga acggtcttca cccaggcgca tgatggagcc tttaccaaat tgtttctcaa
     5341 tctggcccag tgctgccgcc aacgctttct gtttgttttc gtcgatagcc atttttactc
     5401 ctgtcatgcc gggtaatacc ggatagtcaa tatgttctgt tgaaacaatt atactgtatg
     5461 ctcatacagt atcaagtgtt ttgtagaaat tgttgccaca aggtctgcaa tgcatacgca
     5521 gtagcctgac gacgcaccgc atcacggtcg ccgctgaagc attcccgccg ggtaatgcct
     5581 tcaccgcggg cagtggcaaa agcaaaccag acggtgccga caggcttctc ttcactgccg
     5641 ccatccggcc cggcgatacc actaatagac acggcataat cagcacgagc cgctttcagt
     5701 gcacctatcg ccatttccac cacgacgggt tcactcaccg cgccatgctg cgccagcgtc
     5761 tcttcgcgta cgccgatcat ctgcgctttg gcttcgttac tgtaggtgac aaatccgcgt
     5821 tcaaaccagg cggagctacc ggcaatatcg gtaatcactt tcgctaccca accaccggta
     5881 caagactcgg cagttgttac ggttgcgcca cgggctttca gcgcctgccc aacctgttcg
     5941 cttaactgca tcagttcact gtcagtcatc acaacctctg tcagtcaaaa atttatgccg
     6001 gacaagatag cactttcgtc aggaagatgg tgttggcgtt gcaattttac gaggaggatt
     6061 cagaaaaaag ctgattagcc agagggaagc tcacgccccc ctcttgtaaa tagctactgt
     6121 actcgcgcca gcgccacggc ttgtcctaac tgccagaccg ccattgcgta atgggtgctg
     6181 tggttgtaac gggtgatggt gtagaagttc ggcagaccgt accagtactg gtagccggtg
     6241 ccaacatcca gacgcagcag gctggcttgt tgatggttgc ccagcggctg ctgtggcgtt
     6301 aaacctgcgg cggcaagctg cgaaatgctg tacttagttt tgaaaccatt tggcaagcct
     6361 ggagcctgac cgtttgccat tatcgcgacc tgatcgcctt tcacccagcc gtgcgctttg
     6421 aaatagttcg ccacgctacc gatcgcatca accggatccc acaggttgat atgcccgtcg
     6481 ccgctgaaat ctaccgcata ttgtttgtaa gacgacggca taaactgtcc gtagcccatc
     6541 gccccggcaa aggaaccttt cagattgagc ggatcgtcct gctcgtcgcg cgccatcagc
     6601 aggaaggttt ccagctcgcc agagaaatac tccgcgcggc gtgggtagtt aaatgacagc
     6661 gttgccagcg catcgaggat gcgagttttc cccatcacgc gcccccagcg ggtttcaacg
     6721 ccgataatcc cgacgataat ttccggcggt acgccataca cctgccacgc gcgattcaac
     6781 gcatcttcat actgattcca gaaaaccaca ccgttctgca cgttgtccgg cgtaataaat
     6841 tttttgcgat aacgcagcca tgcgccgttc ggacctgatg gcggtttcac cgatgtggtt
     6901 ggtgcctggt tatccatcag ccgcagtacc gaatccagac gcttcgcctg ggagagaatt
     6961 tcctgcaact gctgacgatc gaaaccgtgt ttgttcacca ttttgtcgat gaactgctgg
     7021 gcattcgggt tattagcgaa atcgccgccc atctgcatca cattgtgctg cggctcaagc
     7081 aggaagccgc cagacggcgt tccggtggtc gtttcagtct cagtaggttt tggcttgctg
     7141 ctacaggcgg caagcaacac aaaaagggga agcaatgtta cataacgacg cttgaacatg
     7201 aggggtccat ttaacagatt caaccagggg caagtatggt aaagcatcac accccgcaca
     7261 aggaagcggt agtcactgcc cgatacggac tttacataac tcaactcatt cccctcgcta
     7321 tacttttatt caaactttca aattaaaata tttatctttc attttacgat caaaataaca
     7381 cttttaaacc tttcaacctg attagattag gttgccgttt ggtaataaaa caataaatcc
     7441 tgaaggagag aacaatgata gaaaccatta ctcatggtgc agagtggttt atcgggctgt
     7501 tccaaaaggg cggagaggtg tttaccggga tggtgaccgg cattcttccg ctgttgatta
     7561 gcctgcaggt tatcatgaac gcactgatta attttatcgg tcagcatcgt attgaacgtt
     7621 ttgctcaacg ttgcgccggt aaccctgttt cccgttacct actgttaccg tgcattggca
     7681 cgtttgtctt ttgcaatccg atgaccttaa gcctgggtcg ctttatgccg gaaaagtaca
     7741 aacccagcta ctacgcggcg gcctcttata gctgccactc aatgaatggc ctcttccccc
     7801 atatcaaccc tgcgaactgt ttgtttatct tggcattgcc agcggtctga caacgctgaa
     7861 cctgccactt ggcccactgg cggtgagtta tctgctggtt ggtctggtca ccaatttctt
     7921 ccgcggctgg gtgaccgatc tgaccaccgc catttttgag aaaaagatgg gcattcaact
     7981 tgaacaaaaa gttcacctgg caggagcaac atcatgacgc gtattcggat cgaaaaagga
     8041 acgggtggct ggggcggccc gcttgagctg gaagccacgc cgggcaaaaa aatcgtctat
     8101 atcaccgccg gtacccggcc tgcgattgtt gacaaactgg cacaacttac tggctggcag
     8161 gctattgacg gatttaaaga aggtgaaccc gcggaggcgg aaattggtgt cgcggtaatc
     8221 gactgtggcg gcacattacg ctgcggcatc tatccgaaac gacgtattcc caccattaat
     8281 atccactcga cgggcaagtc cggtccgctg gcgcagtaca ttgtggaaga tatttatgtc
     8341 tctggcgtaa aagaagaaaa catcactgta gtgggtgatg cgacaccaca accctcttcc
     8401 gtgggccgtg actatgacac cagtaagaaa atcaccgaac aaagcgatgg tttactggcg
     8461 aaggtgggaa tgggcatggg gtccgccgtt gccgtgttgt ttcagtctgg tcgtgacacc
     8521 atcgacactg tattaaaaac cattctgccg tttatggcat tcgtctcggc gctcattggc
     8581 atcattatgg cttctggcct tggtgactgg attgcccacg gtcttgctcc gctagcgagc
     8641 catccactgg gtctggtcat gctagcgctc atctgctcct tcccactgct ttcacctttc
     8701 ctcggcccag gcgcagttat cgcacaggtt atcggcgtat tgattggcgt gcagattggt
     8761 ctcggcaata ttccgccgca tctggcttta ccggcactgt ttgccatcaa cgcgcaggcg
     8821 gcttgcgact tcatcccggt cggtttgtcg ctggcggaag cccgtcagga cacggttcgc
     8881 gtcggtgtcc cttctgtact ggtgagccgc tttttaaccg gcgcgccgac tgtactgatc
     8941 gcctggtttg tctccagttt tatctatcaa tagaggctga aacatgaccg ttatttatca
     9001 gaccaccatc acccgtatcg gcgcgagtgc cactgacgcc ctcagcgacc agatgctcat
     9061 cacctttcgt gaaggcgcgc cagcggacct cgaagagtat tgcttcattc attgccacgg
     9121 cgagttgaaa ggtgcactcc atcccggttt gcaattttca ctcgggcagc atcgctatcc
     9181 ggtgaccgct gttggcagcg tggcggaaga caaccttcgc gaactgggtc atgtcaccct
     9241 gcgcttcgat ggtttaaacg aagcggaatt tccgggcact gtccatgtgg caggccctgt
     9301 acccgacgat atcgcgccgg gatcggtttt gaagtttgaa tctgttaagg agtaaaaaat
     9361 gaatcaggtt gccgttgtca tcggtggtgg gcaaacctta ggcgcgttcc tgtgccacgg
     9421 tctggctgcc gaggggtatc gcgtcgcggt tgtcgatatt cagagcgaca aagccgcaaa
     9481 tgtggcacaa gaaattaacg ccgaatatgg tgaaggtatg gcgtacggtt ttggtgctga
     9541 cgccactagc gagcaaagcg ttctggcgct ctctcgtggg gtagatgaaa tctttggtcg
     9601 cgtggatttg ctggtctata gcgccggaat agcaaaagca gcctttatca gcgacttcca
     9661 gctcggcgat tttgaccgtt cgctacaagt gaatctggtg ggttatttcc tgtgtgcccg
     9721 tgaattttcg cgtttgatga tccgcgacgg gattcagggt cgcattattc agatcaactc
     9781 gaaatccggc aaagtgggca gcaaacacaa ctctggctac agcgcagcga aatttggtgg
     9841 cgtcgggctg actcaatcac tggcgctgga tctggcggag tacggcatta cggtacattc
     9901 actgatgctc ggtaacctgc tgaaatcgcg gatgttccag tcactgttgc cacaatacgc
     9961 gaccaagctg ggtatcaaac cggatcaagt cgagcagtat tacatcgaca aagtaccgct
    10021 caaacgcggc tgcgattatc aagatgtgct gaatatgctg ctgttctacg ccagtcctaa
    10081 ggcgtcgtac tgcaccggac agtcgatcaa tgtcaccggc ggtcaggtga tgttctgatc
    10141 aacagcggag atccgttaag gatctccgtg agactataga atgcctgatg cgctacgctc
    10201 atcaggcata caggacttcc gccactacat taaggaaaag ttatggtatc cgcactcatc
    10261 accgtcgccg ttatcgccta gtgtgcgcaa ctggccttag gcggctggca aatttctcgt
    10321 tttaaccgtg ccttcgacac actatgccag caagggcggg ttggcgtggg ccgttccagc
    10381 gggcgcttta aaccgcgggt cgtggtcgcc atcgcgctgg acgatcagca gtgcatcgtc
    10441 gacaccttgt ttatgaaagg actgactgtc ttcgcccgac cgcaaaaaat tcccgcaatt
    10501 accggcatgc atgcgggtga tttacagccc gatgtgatct ttccccatga tccactatca
    10561 cagaatgctc tatcattggc gcttaaactg aaacgtggat aatttcgttg tgaatgttac
    10621 ttgcttgcga agttatcatt ttgaaaccta aatcaggtaa tcacgcccat gaaacctcgt
    10681 cagcgtcagg ccgccattct ggagtatctg caaaagcagg gtaaatgctc ggttgaagaa
    10741 ttggcgcaat actttgacac cacaggcaca accattcgca aagatctggt cattctggaa
    10801 catgccggaa ccgtcattcg tacttatggc ggagtggtgt tgaataaaga ggaatccgat
    10861 ccgcctatcg atcataaaac gctcatcaac acccacaaga aagagctgat tgcagaagct
    10921 gccgttagtt ttatccatga tggcgattcg atcattcttg atgctggcag taccgttttg
    10981 cagatggttc ccctgctctc gcgctttaat aacatcacgg tgatgaccaa cagcctgcat
    11041 atcgtcaatg cgctatccga actggataac gaacaaacta tcctgatgcc aggcggaaca
    11101 tttcgcaaaa aatcggcctc atttcacggg caactggcag agaatgcctt cgagcatttc
    11161 accttcgata aattgtttat gggcaccgac ggcatcgatc tcaatgcggg cgtaaccacg
    11221 ttcaacgagg tttataccgt cagtaaggca atgtgcaatg ccgcgcgcga agtgattttg
    11281 atggcggact catcaaagtt tggccgtaaa agccccaacg tagtttgcag tcttgaaagc
    11341 gtcgataagc taattaccga cgcaggtatc gatccggcgt ttcgtcaggc gctggaagag
    11401 aaagggatcg atgtgatcat aaccggagag agcaatgagt gaagcactac tgaacacggg
    11461 acgtcagacg ttaatgctgg agttgcagga agcaagccgt ttaccggaac gtctgggcga
    11521 tgattttgtt cgcgccgcca atatcatcct gcactgcgaa ggcaaagtgg tggtttcggg
    11581 aattggcaaa tcgggccaca ttggtaagaa aatcgccgca acacttgcca gtaccggcac
    11641 accggctttt tttgtccatc cggcagaagc actgcacggc gatctgggga tgattgaaag
    11701 ccgcgatgtg atgctgttta tctcttactc cggtggcgcg aaggaactgg atctgattat
    11761 tccgcgtctg gaagataaat ctatcgcgct gctggcgatg accggcaaac cgacgtcacc
    11821 gctgggcctg gcggcgaaag cggtgctgga tatctccgta gaacgcgaag cctgcccgat
    11881 gcaccttgcg ccgacctcca gcaccgtcaa taccctgatg atgggtgacg cgctggcgat
    11941 ggcggtcatg caggcgcgcg gttttaatga agaagatttt gcccgctccc acccagctgg
    12001 ggcactgggc gctcgcttgc tgaataaagt gcatcatctg atgcgtcgtg atgacgctat
    12061 cccacaggta gcgttaaccg ccagcgtgat ggatgcgatg ctggaactca gccgcaccgg
    12121 tctggggctg gtggcggtat gtgacgctca acaacaggta caaggcgtct ttaccgacgg
    12181 cgatttacgt cgctggctgg taggcggcgg cgcactcacc acgccagtta atgaagcgat
    12241 gacgacaggc ggcaccacat tacaggcaca aagtcgcgcc atcgacgcca aagaagttct
    12301 gatgaaacgc aaaatcactg ccgcaccagt ggtagatgaa aacggcaaac tcaccggcgc
    12361 aattaacctg caggatttct atcaggccgg gattatttaa tccttcaatc ccagacgttt
    12421 cgccagccga tgcaggttgg cgacgtcagt ttccagcatt cgcgcacagg ctgcccagtt
    12481 gtgatgattt tgtgccagtg cctggcgaat agtttcacgc tggaacgctt ctgtcgcttc
    12541 acgcaggttt tgcttaacaa cgagcaccgc cgccgcttct ggcggcggca acgtcacctc
    12601 aggaaaagca aaatgttgcg cctcaagaat cacttcatcg ccgttgcggg tggctctcgc
    12661 cagcactacc gcccgatgaa tagcatgttc cagttcgcgc acgtttcccg gaaaactgta
    12721 gtgttgcagt aaatttcgcg ccccggcact taataccacg cgggagagcc cctgccgcaa
    12781 acgacactgc tcgcagaaat accccgccag cagaatgaca tcatcgcccc gctcacgcag
    12841 cggcggcacc gaaagtggaa acacgctcag gcgatgaaac aaatcggcgc ggaatcgccc
    12901 tgccagcacc tcttcgcgta agtcgcggtt agtcgccgcc agcacgcgca catcgacccg
    12961 caaactacgg tcatcgccaa cgcgctgaat atcgccatac tgcaacaccc tcagcagctt
    13021 ggcctgcaat gccaacgaca actcgccgat ctcatcgaga aacagagtac cgttatccgc
    13081 catttcaaac ttcccgctgc ggttactgat agcgccagta aacgctcctt tcacatgccc
    13141 gaacaactca ctttccgcca cactttccgg cagtgcggca cagttgagat agaccagcgg
    13201 attcaccgcc cgtggcgagg cttcatgaat cgctttcgcc accagctcct taccggttcc
    13261 cgtctcaccg ctgatcagaa cgttgagatc ggacgccgcc acaatctcaa tctctttttt
    13321 cagttgcgtc atgccagggg acaagccaat catctgcgtc tgtttcaccg cttcaaacgg
    13381 cgtggcatcg cctggcagca tattctggct ttccagttgt tcaatcagca acgcattgct
    13441 taacgctccc gccgccagcg cggcaatcag ccgtaactct tcgtcgctga aaacatcgaa
    13501 ctgatcgggc tgcatcccgt cgagcgtcag tgcgccgatc aggttttgtc cgacaaacaa
    13561 tggcagacca acgcaggcgt gaaccttcag actctcctgc ccaggaatca aaccgtcata
    13621 gggatcgggc aattcgctgt ctgcgggaaa gcgcaccaca tccccggcgc gggcaatcgc
    13681 ttccagccgt ggatgccctt ccagcgcaaa gcgtctaccg agtacatcct ttgccagacc
    13741 gtcgatggca agcggaataa actgccgcga atcgtaacgt agcaacgcag acgcatcgca
    13801 ctccagcacc tgacgtagcg tggtgatcag gcgctgaaaa cgatcctgat gaccaatccc
    13861 acgctgcaat tcgatggcga tattcgccag cacatcaacg gaaaaactca tctttgcctc
    13921 actgtcaatt tgactataga tattgtcata tcgaccattt gattgatagt cattttgact
    13981 actcattaat gagcataatt ttatttatag agtaaaaaca atcagataag aaactggcac
    14041 gcaatctgca attagcaaga catcttttta gaacacgctg aataaattga ggttgctatg
    14101 tctattgtgg tgaaaaataa cattcattgg gtgggtcaac gtgactggga agtgcgtgat
    14161 tttcacggca cggaatataa aacgctgcgc ggcagcagct acaacagcta cctcatccgc
    14221 gaagaaaaaa acgtgctgat cgacaccgtc gaccataaat tcagccgcga atttgtgcag
    14281 aacctgcgta atgaaatcga tctggcggat atcgattaca tcgtgattaa ccatgcagaa
    14341 gaggaccacg ccggggcgct gaccgaactg atggcgcaaa ttcccgatac gccgatctac
    14401 tgtactgcca acgctatcga ctcgataaat ggtcatcacc atcatccgga gtggaatttt
    14461 aatgtggtga aaactggcga cacgctggat atcggcaacg gcaaacagct catttttgtc
    14521 gaaacaccaa tgctgcactg gccggacagc atgatgactt acctgacagg cgacgcggtg
    14581 ctgttcagta acgatgcttt cggtcagcac tactgcgacg agcatctgtt caacgatgaa
    14641 gtggatcaga cggagctttt cgagcagtgc cagcgttact acgccaatat cctgacgccg
    14701 ttcagccgcc tggtaacgcc gaaaattacc gagatcctgg gctttaactt accagtcgat
    14761 atgatagcga cctcccacgg cgtggtctgg cgcgataacc cgacgcaaat tgtcgagctg
    14821 tacctgaagt gggccgctga ttatcaggaa gacagaatca ccattttcta cgacaccatg
    14881 tcgaataaca cccgcatgat ggctgacgct atcgcccagg gaattgcgga aaccgaccca
    14941 cgcgtggcgg tgaaaatttt caacgtcgcc cgaagcgata aaaacgaaat cctgaccaat
    15001 gtcttccgct caaaaggcgt gctggtcggc acttcgacga tgaataacgt gatgatgccg
    15061 aaaatcgccg ggctggtgga ggagatgacc ggattacgct tccgtaacaa acgcgccagt
    15121 gctttcggat ctcacggctg gagcggcggt gcgatggatc gcctttccac gcgcctgcag
    15181 gatgcgggtt tcgaaatgtc gctcagcctg aaagccaaat ggcgaccaga ccaggacgct
    15241 ctggagttat gccgtgaaca cggtcgcgaa atcgcccgtc agtgggcgct cgcgccgctg
    15301 ccgcagagca cggtgaatac ggtagttaaa gaagaaacct ctgccacgac gacggctgac
    15361 ctcggcccac gaatgcagtg cagcgtctgc cagtggattt acgatccggc aaaaggcgag
    15421 ccaatgcagg acgttgcgcc aggaacgccg tggagtgaag tcccggataa cttcctctgc
    15481 ccggaatgct ccctcggcaa agacgtcttt gacgaactgg catcggaggc aaaatgagca
    15541 acggcattgt gatcatcggt tcgggcttcg ccgcccgcca actggtgaaa aatattcgca
    15601 aacaggacgc cactattcca ttaaccctga ttgccgccga cagcatagat gagtacaaca
    15661 aacccgacct cagccatgtt atcagtcagg ggcaacgtgc cgacgacctt acccgccaga
    15721 cggcgggtga atttgccggg cagtttaatc tgcgcctgtt tccgcacacc tgggtgacgg
    15781 atatcgatgc cgaagcccat gtggtgaaaa gccagaataa tcagtggcaa tacgacaagt
    15841 tagtgctggc aaccggtgcc agcgcctttg tcccgcctgt gcctgggcgt gagttaatgc
    15901 tgacgttaaa tagtcagcaa gagtatcgcg cctgtgaaac gcaactgcgg gatgcccgac
    15961 gcgtgttgat tgtcggcggt ggtttgattg gtagcgaact ggcgatggat ttttgtcgtg
    16021 caggcaaagc ggtcacgcta atcgacaacg ctgccagtat tctggcgtca ttaatgccac
    16081 cagaagtaag cagccgcttg cagcatcggt tgacggagat gggcgttcat ctgctgttga
    16141 aatctcagtt gcaggggctg gaaaaaacgg attctggcat tctggcaacg ctggaccacc
    16201 agcgtagtat cgaagtggat gcggtaattg ccgccaccgg actgcgcccg gaaacagccc
    16261 tggcacgacg cgccgggctg acgattaatc gcggcgtttg cgtcgatagt tatctgcaaa
    16321 ccagtaatgc cgatatttac gcgctgggcg attgcgcgga aattaacggt caggtattgc
    16381 cgttcctgca gccgattcaa cttagcggga tggtgctggc aaaaaatctt ctcggcaata
    16441 acacgccgct gaaactaccg gcgatgctgg tgaaaatcaa aacgccggaa ttaccgctgc
    16501 atctggcagg cgaaacccag cgtcaggatt tacgctggca gattaatacc gaacgccagg
    16561 gaatggtggc gcgcggcgtt gacgatgctg accagcttcg cgcctttgtg gtcagtgagg
    16621 atcggatgaa agaggcattt gtattgttga aaacgttgcc ggtgtaggtg ggctactgtg
    16681 cctaaaatgt cggatgcgac gctggcgcgt cttatccgac ctacggggac gcatgtgtag
    16741 gccggataag gcatttacgc cgcatccggc aagggtgtcc aaatgcaaca cgctttatcc
    16801 gttctggact tcacccgcta accaacgcgc cgcagcaata accccctgcc ccagagacaa
    16861 accgccatca cccgccggta aactctgtgg aaagagcaat gtgaaatcag cgagataatg
    16921 cgccagacgt gcacgcagca aacggttatg aataaccccg ccgctaaata ccagcgtagt
    16981 gataccacgc atcgtggcct gctcacgcat caacgcggca aaaccctgcg ccagcgcatc
    17041 atgaaacgcc cacgcgcgtt gattaaccag tgcctgccag ttcagccact gctgccagaa
    17101 agtggcgaga tccagttgat tgtccacccg cggcatcgtc accggatgcg tcactccgtc
    17161 gcatgaggct gcgagcgcct ccagagcaca agccgcttca ccttcataac ttaacgtggc
    17221 tggcgcacag cccagtgccg ccgccactgc atcgaacaac cgcccacacg atgatgccag
    17281 cggcgcgtta attccacgct caatggcccg cgccagtacg ctccagtttt gctgttgcac
    17341 acttgccgtt tcagggtaat tctgccactc cggcacaaag cgcaggcact gcgccagcag
    17401 gtttcgccac ggctgcttcg ccgccaaatc gccacccgga agcgccactg caggcaagcc
    17461 gcccaggtgc tcacattcgc gatagttcac ccgcaggcac tcgccgcccc acaaagcgcc
    17521 attctccccc ataccgatac cgtcgagcgt caaagcaatg acatcaccgc catccagcgg
    17581 ccactgatgc tctgccagac acgccgctgc atgggcatga tgatgcagta ccgtttgcgt
    17641 cggcagattc atttcgctcg cccactggct ggagacatag cccggatgca catcatgcac
    17701 aatgtattgc ggagtgaaat cgtagatgtt ttgcatcagg cgtaacgctt cgcgccactg
    17761 catctggatg ccatcgtcac ttaaatcgcc cagatgctga ctcaacaccg cttgttcacc
    17821 gcgcaccagg cagaaggtat ttttcagatc cgcgccaaga cacagcacag gcggaacatt
    17881 tttaaagccc ggaggcaaaa ccagcgcatc cggcacatac ccccgcgaac ggcgcagcat
    17941 ttcgccgctt tcgcgcacca ctgaatcatc catccgctgc acgatgtcgc ggttatgtag
    18001 caagaatccg tcggcaatgc cctgcaaatc cgccagcgcc tgttcgttgc tgatagctgg
    18061 tggtttaccg ctcaggttgc cggaggtcat caccagcggg cattgcagtt cctgtaacag
    18121 caaatgctgg agcgggttcg caggcaacat caccccgact tcgttaaggc caggggcgat
    18181 atcatcacaa agctcaggta cgtatttttt atccaccagc acaatcggcg cagcgggcgt
    18241 ggtaagcaac tggcgcgcag cgtctggtaa actttctgcc actggcaaca tgactgccag
    18301 cggtttcgcc gggcgatgtt tgcgctcccg cagtgtcgcc accgcgttac tgttacgtgc
    18361 atcgcaggca agatgaaatc cgccaatccc tttgatggcg acaatgttgc ccatttttaa
    18421 ctgtgcgata gctgcctgta atgccgcctc ttgttctgca tgttcaccat gacttaccca
    18481 ttcaagatgc gggccacact ccgggcaggc caccggctgg gcgtggaagc gacgatcgag
    18541 cgggtcgcgg tactctttgt cacaggccgg acatagcgga aacgccgcca tcacggtaaa
    18601 cgggcggtcg taaggcatcg cgcgaataat ggtgaaacgc gggccgcagt gggtacagtt
    18661 gataaacgga taacgataac gccgctcgcc tggggtattc atttcggcaa ggcaagcagg
    18721 gcaagtggcg gcatccggga caatttgcgt attcatggtg ccgccggtgc tctggcggat
    18781 ggtgaactcg gtgggcagtt gtgaccagat aaacggctca cgctcgacgc tatcaatacg
    18841 cgccagtggc ggacagtgct gatgcaattg aacaagaaac gtttccgggt cttccagcag
    18901 ccggacttct acgccatcgc cgtcattaca tacatcgccg tgaagattta attgctgtgc
    18961 cagctgccag acaaacggac gaaaaccgac gccctgcact ttgccacgaa tacgcagttg
    19021 gacaccgcaa gatgtgtttt ttgccattga gttattcccg ccatcatgaa ttgcgtaacc
    19081 cgccctgccg gacacgacag cgtcgtatcc ggcagtctca ggtcggcgat cccgcccgct
    19141 ccgtattcta cgaatatttc cgggaattcc tttgatgcca gaacagttct gtaagatttt
    19201 tagaacatca gcgccgtacg gcggcgtttt tctgcgctca gttgttcaag tttattacga
    19261 tcgacacaaa tcagcgcatg ggtcgggcaa gccgccatac acgccgggcc gtcttcacga
    19321 tggttgcaca ggtcgcattt attggcttcg gctttgtcag cccgtacatt cagacccgcg
    19381 ccgctgttgc gaatcacagg acgtaccacc acttccatcg cgccatacgg gcaagccacc
    19441 acgcaggttt tgcaaccaat gcaacgttcc tgcatcacag gaacaaaccc tttatcacgg
    19501 ctgatagcac cattcgggca gacgttagcg cacggtgcat cttcacactg acggcaaact
    19561 gtcgccgtgg aaatgttcac acctttaatg acatggatac gcggtaaaaa tgtttccggg
    19621 gtcagcgatg cacagtcctg attttcctga tgagaaacca cgcacgccac ttcacaggta
    19681 cggcaaccaa tacatttact cgcgtcagca atgatgaaac ggttcatcaa attctccagc
    19741 aatgacagtt aatgcgccga tacattcaca aatcatgcca gtttttaatt tgctgttatt
    19801 taaggaaatt aatttctgta atgcaggaaa aaagatgtca tcgacactag tgacgatgac
    19861 atgtgatgac aatgtttatc gcgaaagggc aatgagtgag tcgcggcgga tcagttttcc
    19921 gctaaaggtt ttcggcggtg agaaatcccc gccatcgagc ataaaaatca gccgtccaat
    19981 aatttcctga atcatctcag tcaccggaat ttttacgctg gagagcgccg gaacgatata
    20041 gggggcaatt gcgatatcat cgaatccgat aactgacacc tgctctggca ccgctacgcc
    20101 gcgctcgtgc aacgctttga tcgcacctat cgccatatcg tcgttactgg caactaacgc
    20161 gctaaattta gcccctcgtt cgagcaacgt ttctacccct tcggccccgc tggcgggcgt
    20221 ccatttaccg ttagcgataa gtttttcatt gagcgcaata ccatgctgcg acagcgcgtc
    20281 tttatacccg gcaagacgtt caatgctggt gggggaatcc atcgagccgg taaggaaagc
    20341 aatctcctga tgcccggcgt ttatcaactc tgccacggcg ttaaagctgg tctgtttatg
    20401 atcgcaccag acgctatggc tgctattatt gcgcaggcgg cgattaagca ccattatcgg
    20461 ctgactgtgc gcgtcaatga tgtcatcaat ctcatccacg cttaaaaaac gcgggtaaat
    20521 cataatggcg tcgcagcgca aatccagcag atactgaatc gcctggcgct cttcttcggc
    20581 gctgtgttta ccatctgcca atagcaactg tcgccctttc tcttccgcca ttcgcgcggc
    20641 atgaaacagt aattcactaa aataaatgcc gtgataaagc gtgttggtca ctaccagccc
    20701 cagcgtctga gtactcttcg ccgacagatt gcgggccagc aaatttgggc ggtaactgct
    20761 ctcttctacc gcctgaaaca cgcgatcttt agtctcctgg ctgacgtagc cattacctga
    20821 aagcacgcgg gaaacggtcg cttttgaaac cccggcgcgc ttcgccactt ccagcatcgt
    20881 cgtcatcatt ttcatccctt tacacgcaat caacgcagtg tactgcaccg tttgccgatt
    20941 gtccttgcgc aatcggctcc agcggataag acgaggtcgc cgtgccggtt ttgatgactt
    21001 ttttgataaa ggtaaacatg gcgattcctt atttcagcgg cgagttttta cgctcaatgc
    21061 tgtaacgctc gagttctttg tacggcacca ctttgctctt cttcttacgc acatcgacta
    21121 cggtcatgcg gtcggtacag gagtagcaag ggtccaggct accgataatc agcggcgcat
    21181 cggaaacggt attgccacgc agcatgtagc gcagggtcgg ccagttcgcg taggtcgcgg
    21241 cacggcagcg ccagcggtac agcttctggt tgtcgccggt catgctccag tggatatcgt
    21301 cgccgcgcgg cgcttcggca aagcccagcg cgaaacggtg cggaatgtag gtaaagcctt
    21361 ccaccatcag cggaccgccc ggcaggttat ccagaccgta gtcgatcata ttcagcgcgg
    21421 tatagacctc gttgattcgc actttcagac gggaaataac gtcgcagccc tgctcgctgt
    21481 ggacttccat tggcagcagg ccataaccga caaacgggtg atcggcgcga gtatcgcggg
    21541 cgagaccgct ggcgcgaacc atcgggccaa cgttactgaa atcgcgggcg atttccgggt
    21601 ccagacgacc aatgccgacg gtgcgttgtt ccatgttcgg cgtactcagc agcacatcca
    21661 ccagctcctg cacttcacga cgcatttgtt gcgccagctg gcgggtctgg atcatgtcat
    21721 ctttcagcag atcgcgacga atcccgccga tcaggttcag gccgtaggtt ttacgcgcac
    21781 cggtaaggat ctctgccatt ttcatggagg tttcacgcac gcggaagaac tgcataaagc
    21841 cggagtcgaa gccggtaaag tgacaggcga ggccgaggtt gagcagatgc gggtgcaggc
    21901 gttccacctc cagcagaatg gcgcggatca tctgcgcgcg ttctggcacc tgaataccca
    21961 tcgcgttttc caccgacgtg gtgtaggcgg tgctgtgggc aaagccgcag atcccgcaca
    22021 cacggtcaga gaggaaggtc acttcgttat aacccatgcg ggtttccgcc agtttttcca
    22081 taccgcgatg gacgtagaac agacggtagt cggcgtcgat aatgttttcg ccatcgacga
    22141 acagacggaa gtgacccggt tcgtcagaag tgacgtgcag cggaccaatc ggcacgacgt
    22201 tgtttttctt gtcgcccagt tcgttgatga actcgtaggt ttcagcatcg gtagtcggtg
    22261 ccggacgctg acgataatcc atgctgtctt tacgcagcgg ataaagttca tccggccagt
    22321 catccggcag caccagacga cgttcatccg gcagaccaac cggaatcaaa ccgtacatat
    22381 cgcgcacttc gcgctcgccc cacaccgccg ccggaacgcg cggcgtcacg gacggatact
    22441 ccggtttgtt ggcgtcaact tcgacgcgaa ccgttaccca acacttggtg cccttctcca
    22501 tcgacagcac gtaataaacg gcgtaatgac cattcagttt gcgttcgtcg ttaccaaaca
    22561 gcaccgacag ccagccaccc tgcttgtagt agagaaactc caccacttcc ggcagatagt
    22621 tcacctttac ggtgacagtc agctgatctt tggtctgcca ggcgtggtcc agcacgacgc
    22681 ctagaaatgc ctcattcagc gcggcgagat aatgttgacc taatttttct tcagacatgc
    22741 tcaaactctc tttaatcacg ccgccagcaa ggagacgaac gctaaaaatg caaagccaaa
    22801 cccagcccag gtaatgcgcg gagtaatatc aagacgcaga cgggccatgc tgttttcgaa
    22861 cagcgcgata accaggacgc cgaccaccag tttgacgatg gcaatcacca gcgccagcag
    22921 cagtccaccg acggtgaagg tttccatttg tccccacgga ataaacaccc cgacgaacat
    22981 ctgcaacacc accagctgtt tcaggctgat accccatttc atgacgccaa agccgctacc
    23041 gctgtattcg gagagcggac cttcctgcaa ctcctgctcg gcttccgcca ggtcgaacgg
    23101 cagtttgccc atttcgataa aagtggcgaa cgcacaggcg cagagcgcca gcaccagcgg
    23161 gatgctttgg ctcagcggcc agtgataaac ggtgtcggta atgttgctga tgttggtgga
    23221 accggcaacc tgtgcggcaa cccacagacc aagcagcaac atcggttcga ccagcacgcc
    23281 aagcatcgct tcacggctcg caccgatagc ggtaaacggg ctaccggtat ccagaccgga
    23341 aatggcaaag aagaaacgcg cgatggcgaa gagatacagt aaggtgatca aatcgcccag
    23401 ttgcggcagc ggagaaccga cggtcaccac cggcagcgca gtagcgatag tcagcatgac
    23461 gcccaccatc acatacggcg tcaggcggaa catccagcca gaggcatccg ggccaacgct
    23521 ctggcggccc agcagtttga taatgtcgcg atactcctgc aacacgcccg gcccgcgacg
    23581 gttatgcaga cgggcgcgcg ccacgcgggt aataccggag agcagcggcg caacggcaaa
    23641 taacaccagc gcctgaatta acggatataa aacactcatt ctcaggctcc tcgtgaaaca
    23701 ataatcacca ccagtaccgc cagttcaacc agcgccatcc ggcggaacag caacgcacta
    23761 ccttcgcact gccagcccgg cactagcgac accggattca gccatttgcg cagtttcagc
    23821 actggcgcaa acgcctgttt aaccggcatg gcaaaaccgt gagcggtaat caccattgat
    23881 ttttcatggt cgtagccgca cacccaggcc gcaccgcggg aacgtgacgg caggcggtcg
    23941 cctttgcaaa tcgccataat gatgaatggc agcagcgggc aggcaatcag cagcaatgtg
    24001 atcattggtt gagaaacggt ggtgttagca ggctccagag gcagaggtac agcagcagag
    24061 agcatcggca gcagccacgg cgcagcaaca ccgccaatta cgcagcaaat cgccagtgcc
    24121 actacgctta cgctcatcag gagcggcgca caggtggcgt tttcggcttc tttggtacgc
    24181 ggcgcgccga ggaacgtgac gccatagact ttcgccatac acatcaccgc cagcgcaccg
    24241 gtaattgcca gacccacagc gagcagcggc cccagcagac gggcaacaaa cgcgccgcta
    24301 ttgctcagtt tgaagaagga ttgatagata acccattccc cggcaaaccc gttcagtggc
    24361 ggcagcgcag ccattgccat caacccgact aacatggcga tggagataac cggcattttc
    24421 ttgccaatac caccgagttt ttcgatatcg cgatgaccgg tacggaacca gacgctcccc
    24481 gccccgagga acagtacgct tttgaacagg ctatggttaa gcagatggta cagaccaccg
    24541 accaggccaa gagcaatcag cgccggttgt tcgagcgcga tacccgttac gccagcgccc
    24601 agccccagcc ccagccccag caggataatg ccgatatttt ccagggtgtg gtaagccagc
    24661 aggcgctgga tgttgtgttc catcaacgca tacagaccac cgacgaacgc ggtgatcatg
    24721 ccgagcacca gcagcgcgat cccccaccac agcggtgcat taccgcccag cagtgacagg
    24781 gttaacatgc ccagcaggcc aattttcatg actaccgtag aaaacaacgc ggcagctggc
    24841 gcagaggcgt tagcatgtgc ctgcggcacc cagccgtgca gcggaataat cccagccagc
    24901 aggccaaagc caatcacacc gagcagccag atatcggaac cgagcggcag ctgttgcata
    24961 cgcatatcca gcaggcgcag atccagcgtg ccataacgct gccacagcag ccagcaagca
    25021 atcaccagca gcagagtgcc aagacgcccc agcgcaaacc acagtttgcc ctctttgctg
    25081 ttgctggtga ggaacaccgc acacaaggcc atgacttcgg ccattaccac gaacatgccg
    25141 aggttgctgg cgatgacggc gcagacggcg gcagccatca acatattgat ctgcaagccg
    25201 ttgcacttca cctgcgcgtg gcgatgccag tcaatgttat agaggctgac aaacagaccg
    25261 caaaggccga gggtaatcag ccaaatcgcg ttaagcgggg agatttgcac atcgtagctt
    25321 accagcgaca gcgcaccgct cacgccaacc gcgccagtca gtacagtgaa gcccgcggct
    25381 gccgtataca gactaccaac cgcgccgcca attccggcta tccagccact tagcgctttt
    25441 tgaaaagaga aatgccagaa cagcggcggc gacaaaccat gccacgccgc tattgatcag
    25501 ggaaattgcg ctcatttagc ctctccactt tgagcctgct gaaacaaggt gagatcgcca
    25561 aagtccgtgt taaaggtcag ctcacgctta cgtttgctga cgcgggcgat atcggtgtta
    25621 tccaccagat gcagggcttt agtcgggcac atccgcacac aggccggacc ttgttcatca
    25681 aagctacaaa ggtcacattt gacggcgatc gcgcgaatac ctggcaccca gtcaagcaat
    25741 gtgctgacac gcgccggagc aggcggtgcc ggtggcgctt tcggggtatt ggcgtttgcc
    25801 ggaatatcca gcggacggct gccggaaaat tcaattgcgc caaacgggca ggcgatgccg
    25861 cacagtttgc agcttacgca cagactttca ttcaactgca cggccccatc aacgcgggtg
    25921 atggcgttaa ccgggcagac caccgcgcag ggtgcatctt cacagtggtg acagagctgc
    25981 ggcgcagatt ctttttcatt gagcatcact ctcaggcgcg gcattgattg caggccgtgc
    26041 tggcgatgcg tctctgaaca ggcggcctca caggtgtggc agccgataca gagcgtggag
    26101 tcggcaatta caaaacgatt caccaggcat tcctcaggtg attgtcattt ttgacgaaaa
    26161 catgccgatg aaatgtcatt ttcgacactc atcgacacgc ccatccccaa acaggcgtaa
    26221 cgcctgcaaa acgggcaaag cctcagctca tgctgccggg ctttgtccct ttaccagttg
    26281 gcttaaattc accggcacat tgttttcaat ggcggtgtat aagctgtcgt aaacgccagc
    26341 gattttttcg agatagtgtt ccagaacctg ttcgcgatcg cggatgttga ccttgccatc
    26401 aatcatgccg tcaatgctca gttgcgcctg cgcaatccgc tgtttgtctt cgccatcttt
    26461 tgtttcgacg taatactcga tggtgtggct gttaaagcca tccgccaggg tgacgtaaat
    26521 acgaaaatgt tcaaaaagga cgaaacagag ttctttgtcc ggcgcgcagc tcatggcgtg
    26581 atacaggcgc gctttcgata acgtgatccc ctgaaccagc gaattgcagt agatgtgcca
    26641 ctggtcctgt aggcgacgat gccgctgtgc gatgtaatcg gctttctcgc ttatttccca
    26701 aatagtcatt gtcaggttac tcgtttaaca gagatgccag ctttaagcat tttttgtgcc
    26761 aacttttaat ttattgttat taaagagatt tttaagctaa agatgaattt cgtcgccgtg
    26821 tcgacgtgtc atttcgacat catcgacatt attcaccgca gggataatca acactggcgc
    26881 aattattgct tgtagctggc aatagttaat gggaggcgat atgcacgaaa taaccctctg
    26941 ccaacgggca ctggaattga tcgaacagca ggccgcaaaa cacggcgcaa aacgcgtaac
    27001 tggggtctgg ctcaaaattg gcgcattttc ttgtgtcgaa accagctctc ttgccttttg
    27061 ttttgatctg gtttgccgcg gcagcgtggc ggaaggttgt aaactgcacc tcgaagaaca
    27121 agaggccgaa tgctggtgtg aaacatgcca acagtatgtg acgctactga cccagcgcgt
    27181 ccgccgctgt ccacagtgtc atggtgacat gctacagatt gtggcagacg acggtttaca
    27241 gattcggcgg atagaaatag accaggagtg agcgatgtgt acaacatgcg gttgcggtga
    27301 aggcaacctg tatatcgagg gtgatgaaca taaccctcat tccgcgtttc gtagcgcgcc
    27361 atttgccccg gcggcacgcc cgcagatgaa aatcaccggc attaaagcgc ctgaatttac
    27421 ccccagccag actgaagaag gcgacctgca ttacggtcat ggcgaagcgg gcactcacac
    27481 accgggcatg agccagcgtc ggatgctgga agtcgaaatt gacgtgctgg acaaaaacaa
    27541 ccgtctggct gaacgcaacc gcgcgcgctt tgctgcccgc aagcaactgg tgctcaacct
    27601 ggtttccagc cctggttccg gtaaaaccac cctgctgacg gaaaccttaa tgcgcctgaa
    27661 agacagcgtt ccgtgcgcgg ttattgaagg cgaccagcaa accgtgaacg atgccgcacg
    27721 cattcgcgct accggtacac cagcgattca ggtgaacacc ggtaaaggct gccatcttga
    27781 cgcacagatg attgccgacg ccgcaccgcg tctgccactg gacgataacg gtattctgtt
    27841 tatcgaaaac gttggcaacc tcgtatgccc ggccagcttc gatctcggtg aaaaacacaa
    27901 agtagcggtg ctttccgtta ccgaaggtga agacaaaccg ctgaaatatc cgcatatgtt
    27961 tgccgccgcc tcgctgatgc tgctcaacaa agttgacctg ttgccgtatc tcaactttga
    28021 cgttgagaag tgcatcgcct gcgcccgcga agtcaatcca gaaattgaaa tcatccttat
    28081 ttccgccacc agcggcgaag ggatggacca gtggctgaac tggctggaga cacagcgatg
    28141 tgcataggcg ttcccggcca gatccgcacc attgacggca accaggcgaa agtcgacgtc
    28201 tgcggcattc agcgcgatgt cgatttaacg ttagtcggca gctgcgatga aaacggtcag
    28261 ccgcgcgtgg gccagtgggt actggttcac gttggctttg ccatgagcgt aattaatgaa
    28321 gccgaagcac gcgacactct cgacgcctta caaaacatgt ttgacgttga gccggatgtc
    28381 ggcgcgctgt tgtatggcga ggaaaaataa tgcgttttgt tgatgaatat cgcgcgccgg
    28441 aacaggtgat gcagttaatt gagcatctgc gcgaacgtgc ttcacatctc tcttacaccg
    28501 ccgaacgccc tctgcggatt atggaagtgt gtggcggtca tacccacgcc atttttaaat
    28561 tcggcctcga ccagttactg cctgaaaacg ttgagtttat ccacggtccg ggttgcccgg
    28621 tgtgcgtact gccgatgggt agaatcgaca cctgcgtgga gattgccagc catccggaag
    28681 tcatcttctg tacctttggc gacgccatgc gcgtaccagg gaaacaggga tcgctgttgc
    28741 aggcaaaagc acgcggtgcc gatgtgcgca tcgtttactc gccgatggat gcgttgaaac
    28801 tggcgcagga gaatccaacc cgcaaagtgg tgttcttcgg cttaggtttt gaaaccacca
    28861 tgccgaccac cgccatcact ctgcaacagg cgaaagcgcg tgatgtgcag aatttttact
    28921 tcttctgcca gcacattacg cttatcccga cgctgcgcag tttgctggaa cagccggata
    28981 acggtatcga cgcgttcctc gcgccgggcc acgttagtat ggttatcggc actgatgcct
    29041 ataattttat cgccagcgat tttcatcgtc cgctggtggt cgctggtttc gaaccccttg
    29101 atctactgca aggcgtggtc atgctggtgg agcagaaaat agcggcccac agcaaggtag
    29161 agaatcagta tcgtcgggtg gtaccggatg ccggtaacct gctggcgcaa caggcgattg
    29221 ccgatgtgtt ctgtgtcaac ggcgacagcg aatggcgcgg cttaggcgtg attgaatctt
    29281 ctggcgtgca cctgacgccg gattatcaac gattcgatgc cgaagcacat ttccgcccgg
    29341 caccgcagca ggtctgcgat gacccgcgcg cgcgttgtgg tgaggtatta acgggcaaat
    29401 gtaagccgca tcaatgcccg ctgtttggta acacctgtaa tcctcaaacc gcatttggtg
    29461 cactgatggt ctcctccgaa ggagcgtgcg ccgcgtggta tcagtatcgt cagcaggaga
    29521 gtgaagcgtg aataatatcc aactcgccca cggcagcggc ggccaggcga tgcagcaatt
    29581 aatcaacagc ctgtttatgg aagcctttgc caacccgtgg ctggcagagc aggaagatca
    29641 ggcacgtctt gatctggcgc agctggtagc ggaaggcgac cgtctggcgt tctccaccga
    29701 cagttacgtc attgacccgc tgttctttcc tggcggtaat atcggcaagc tggcgatttg
    29761 cggcaccgcg aatgacgttg cgggcagtgg cgctattccg cgctatctct cctgtggctt
    29821 tatcctcgaa gaaggattgc cgatggagac actgaaagcc gtagtgacca gcatggcaga
    29881 aaccgcccgc acggcaggca ttgccatcgt tactggcgat actaaagtgg tgcagcgcgg
    29941 cgcggcagat aaactgttta tcaacaccgc tggcatgggc gcaattccgg cgaatattca
    30001 ctggggcgca cagacgctaa ccgcaggcga tgtattgctg gttagcggta cactcggcga
    30061 ccacggggcg actatcctta acctgcgtga gcagctgggg ctggatggcg aactggtcag
    30121 cgactgcgcg gtgctgacgc cgcttattca gacgctgcgt gacattcccg gcgtgaaagc
    30181 gctgcgtgat gccacccgtg gtggtgtaaa cgcggtggtt catgagttcg cggcagcctg
    30241 cggttgtggt attgaaattt ctgaatcagc gctgccggtt aaacctgccg tgcgcggcgt
    30301 ttgcgaattg ctgggactgg acgccctgaa ctttgccaac gaaggcaaac tggtgatcgc
    30361 cgttgaacgc aacgcggcag agcaagtgct ggcagcgtta cattcccatc cactggggaa
    30421 agacgcggcg ctgattggtg aagtggtgga acgtaaaggt gttcgtcttg ccggtctgta
    30481 tggcgtgaaa cgaaccctcg atttaccaca cgccgaaccg cttccgcgta tatgctaata
    30541 aaattctaaa tctcctatag ttagtcaatg accttttgca ccgctttgcg gtgctttcct
    30601 ggaagaacaa aatgtcatat acaccgatga gtgatctcgg acagcaaggg ttgttcgaca
    30661 tcactcggac actattgcag cagcccgatc tggcctcgct gtgtgaggct ctttcgcaac
    30721 tggtaaagcg ttctgcgctc gccgacaacg cggctattgt gttgtggcaa gcgcagactc
    30781 aacgtgcgtc ttattacgcg tcgcgtgaaa aagacacccc cattaaatat gaagacgaaa
    30841 ctgttctggc acacggtccg gtacgcagca ttttgtcgcg ccctgatacg ctgcattgca
    30901 gttacgaaga attttgtgaa acctggccgc agctggccgc aggtgggcta taccccaaat
    30961 ttggtcacta ttgcctgatg ccactggcgg cgggagggca tatttttggt ggctgtgaat
    31021 ttattcgtta tgacgagcgt ccctggagcg aaaaagagtt caatcgtctg caaacattta
    31081 cgcagatcgt ttctgtcgtc accgaacaaa tccagagccg cgtcgtcaac aatgtcgact
    31141 atgagttgtt atgccgggag cgcgataact tccgcatcct ggttgccatt accaacgcgg
    31201 tgctttcccg cctggatatg gacgaactgg tcagcgaagt cgccaaagaa atccattact
    31261 atttcgacat tgacgatatc agcattgtct tacgcagcca ccgtaaaaac aaactcaaca
    31321 tctactccac tcactatctt gataaacaac atcccgccca cgaacagagc gaagtcgatg
    31381 aagccggaac cctcaccgaa cgcgtgttca aaagtaaaga gatgctgctg atcaatctct
    31441 acgagcggga cgatttagcc ccctatgaac gcatgttgtt cgacacctgg ggcaaccaga
    31501 ttcaaacctt gtgcctgtta ccgctgatgt ctggcgacac catgctgggc gtgctgaaac
    31561 tggcgcaatg cgaagagaaa gtgtttacca ctaccaatct gaatttgctg cgccagattg
    31621 ccgaacgtgt ggcaatcgct gtcgataacg ccctcgccta tcaggaaatc catcgtctga
    31681 aagaacggct ggttgatgaa aacctcgccc tgaccgagca gctcaacaat gttgatagtg
    31741 aatttggcga gattattggc cgcagcgaag ccatgtacag cgtgcttaaa caagttgaaa
    31801 tggtggcgca aagtgacagt accgtgctga tcctcggtga aactggctcg ggtaaagagc
    31861 tgattgcccg tgcgatccat aatctcagtg ggcgtaataa tcgccgcatg gtcaaaatga
    31921 actgcgcggc gatgcctgcc ggattgctgg aaagcgatct gtttggtcat gagcgtgggg
    31981 cttttaccgg tgccagcgcc cagcgcattg gtcgttttga actggcggat aaaagctctc
    32041 tgttcctcga cgaagtgggc gatatgccac tggagttaca gccgaagttg ctgcgtgtat
    32101 tgcaggaaca ggagtttgaa cgtctcggca gcaacaaaat cattcagacg gacgtacgtt
    32161 taattgccgc gactaaccgc gatctgaaaa aaatggtcgc cgaccgagag ttccgtagcg
    32221 atctctatta ccgcctgaac gtattcccga ttcacctgcc gccactacgc gagcgtccgg
    32281 aagatattcc gctgctggcg aaagccttta ccttcaaaat tgcccgtcgt ctggggcgca
    32341 atatcgacag cattcctgcc gagacgctgc gcaccttgag caacatggag tggccgggta
    32401 acgtacgcga actggaaaac gtcattgagc gcgcagtatt gctaacacgc ggcaacgtgc
    32461 tgcagctgtc attgccagat attgctttac cggagcctga aacgccgcct gccgcaacgg
    32521 ttgtcgccca ggagggcgaa gatgaatatc agttgattgt gcgcgtgctg aaagaaacca
    32581 acagcgtggt tgccgggcct aaaggcgctg cgcaacgtct ggggctgaaa cgcacgaccc
    32641 tgctgtcacg gatgaagcgg ctgggaattg ataaatcggc attgatttaa ctgcaaattg
    32701 ccggacagat ctgcctgtcc ggcatactat tcacgaggtt ttttcggacg atatttttcc
    32761 ggcagttctg gcaccggacg cttgtcatcg atgagatgac gcacggttaa gatcggatga
    32821 cgccacagca ttctcggccc ggcccaacgc ataatctgtt tcatctcttc acgctttgca
    32881 ggctggtaac agtgcaccgg acactgctta caggctggtt tctcttcgcc gaacacacat
    32941 ttatccagcc gcttttgcgc gtaaacaaac aacgcctcgt aatgctccgg ctccgctgac
    33001 gcctgcgggc atttcgcttg ataaagatcg atcatttttt taatcgtcag tttttcacga
    33061 gagatacgct tgccggacat gctgcctcca cctcattaag atgcatttat attacatctt
    33121 aatc
//
LOCUS       DAJTQY010000037        32630 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.037, whole genome shotgun sequence.
ACCESSION   DAJTQY010000037 DAJTQY010000000
VERSION     DAJTQY010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32630
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.037"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            698..1126
                     /gene="uspC"
                     /locus_tag="OO349_001834"
     CDS             698..1126
                     /gene="uspC"
                     /locus_tag="OO349_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HCR5866933.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASNPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCHKHH
                     FNLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
     gene            complement(1133..2547)
                     /gene="otsA"
                     /locus_tag="OO349_001835"
                     /pseudo
     CDS             complement(1133..2547)
                     /gene="otsA"
                     /locus_tag="OO349_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
     gene            complement(2522..3322)
                     /gene="otsB"
                     /locus_tag="OO349_001836"
     CDS             complement(2522..3322)
                     /gene="otsB"
                     /locus_tag="OO349_001836"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HCR5866934.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITAALQQKRENNRSDDYESFSRSI"
     gene            complement(3489..4475)
                     /gene="araH"
                     /locus_tag="OO349_001837"
     CDS             complement(3489..4475)
                     /gene="araH"
                     /locus_tag="OO349_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HCR5866935.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGVLCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGIPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            complement(4490..6004)
                     /gene="araG"
                     /locus_tag="OO349_001838"
     CDS             complement(4490..6004)
                     /gene="araG"
                     /locus_tag="OO349_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP binding protein
                     AraG"
                     /protein_id="HCR5866936.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLAIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIGNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKALGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEARKAEGIIPVHSVRDNINISARRKHVLGGCVINNSWEENNADQHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(6074..7063)
                     /gene="araF"
                     /locus_tag="OO349_001839"
                     /pseudo
     CDS             complement(6074..7063)
                     /gene="araF"
                     /locus_tag="OO349_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
     gene            7860..8363
                     /locus_tag="OO349_001840"
     CDS             7860..8363
                     /locus_tag="OO349_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HCR5866937.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(8443..8694)
                     /locus_tag="OO349_001841"
     CDS             complement(8443..8694)
                     /locus_tag="OO349_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HCR5866938.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTHHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(8809..8895)
                     /gene="azuC"
                     /locus_tag="OO349_001842"
     CDS             complement(8809..8895)
                     /gene="azuC"
                     /locus_tag="OO349_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HCR5866939.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            9158..9481
                     /locus_tag="OO349_001843"
     CDS             9158..9481
                     /locus_tag="OO349_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HCR5866940.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            9652..10149
                     /gene="ftnA"
                     /locus_tag="OO349_001844"
     CDS             9652..10149
                     /gene="ftnA"
                     /locus_tag="OO349_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HCR5866941.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(10186..10425)
                     /locus_tag="OO349_001845"
     CDS             complement(10186..10425)
                     /locus_tag="OO349_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HCR5866942.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGVTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            10615..11826
                     /gene="tyrP"
                     /locus_tag="OO349_001846"
     CDS             10615..11826
                     /gene="tyrP"
                     /locus_tag="OO349_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HCR5866943.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(11888..12553)
                     /gene="yecA"
                     /locus_tag="OO349_001847"
     CDS             complement(11888..12553)
                     /gene="yecA"
                     /locus_tag="OO349_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HCR5866944.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(12749..12835)
                     /locus_tag="OO349_001848"
     tRNA            complement(12749..12835)
                     /locus_tag="OO349_001848"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12799..12801),aa:Leu,seq:taa)
     gene            complement(12848..12921)
                     /locus_tag="OO349_001849"
     tRNA            complement(12848..12921)
                     /locus_tag="OO349_001849"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12887..12889),aa:Cys,seq:gca)
     gene            complement(12976..13051)
                     /locus_tag="OO349_001850"
     tRNA            complement(12976..13051)
                     /locus_tag="OO349_001850"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13016..13018),aa:Gly,seq:gcc)
     gene            complement(13203..13751)
                     /gene="pgsA"
                     /locus_tag="OO349_001851"
     CDS             complement(13203..13751)
                     /gene="pgsA"
                     /locus_tag="OO349_001851"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HCR5866945.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYVGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(13808..15640)
                     /gene="uvrC"
                     /locus_tag="OO349_001852"
     CDS             complement(13808..15640)
                     /gene="uvrC"
                     /locus_tag="OO349_001852"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HCR5866946.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAACIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(15637..16293)
                     /gene="uvrY"
                     /locus_tag="OO349_001853"
     CDS             complement(15637..16293)
                     /gene="uvrY"
                     /locus_tag="OO349_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HCR5866947.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVAGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            16589..16765
                     /locus_tag="OO349_001854"
     CDS             16589..16765
                     /locus_tag="OO349_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5866948.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTT
                     NFHCNMTRKINEYA"
     gene            16752..16976
                     /locus_tag="OO349_001855"
     CDS             16752..16976
                     /locus_tag="OO349_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HCR5866949.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(17009..17629)
                     /gene="sdiA"
                     /locus_tag="OO349_001856"
                     /pseudo
     CDS             complement(17009..17629)
                     /gene="sdiA"
                     /locus_tag="OO349_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
     gene            complement(17859..18611)
                     /gene="yecC"
                     /locus_tag="OO349_001857"
     CDS             complement(17859..18611)
                     /gene="yecC"
                     /locus_tag="OO349_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HCR5866950.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKLLLQ"
     gene            complement(18608..19276)
                     /gene="tcyL"
                     /locus_tag="OO349_001858"
     CDS             complement(18608..19276)
                     /gene="tcyL"
                     /locus_tag="OO349_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HCR5866951.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(19291..20277)
                     /gene="dcyD"
                     /locus_tag="OO349_001859"
     CDS             complement(19291..20277)
                     /gene="dcyD"
                     /locus_tag="OO349_001859"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HCR5866952.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDEVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(20382..21182)
                     /gene="tcyJ"
                     /locus_tag="OO349_001860"
     CDS             complement(20382..21182)
                     /gene="tcyJ"
                     /locus_tag="OO349_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5866953.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKESGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(21270..21821)
                     /gene="fliZ"
                     /locus_tag="OO349_001861"
     CDS             complement(21270..21821)
                     /gene="fliZ"
                     /locus_tag="OO349_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HCR5866954.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDEKGWQTEQQSWAALCRFCGDLHCKTQSDFFDIIGFKKFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(21867..22586)
                     /gene="fliA"
                     /locus_tag="OO349_001862"
     CDS             complement(21867..22586)
                     /gene="fliA"
                     /locus_tag="OO349_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HCR5866955.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(22751..24403)
                     /gene="fliC"
                     /locus_tag="OO349_001863"
     CDS             complement(22751..24403)
                     /gene="fliC"
                     /locus_tag="OO349_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HCR5866956.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDL
                     KKIDSDTLGLNGFNVNGGGAVANTAASKADLVAANATVVGNKYTVSAGYDAAKASDLL
                     AGVSDGDTVQATINNGFGTAASATNYKYDSASKSYSFDTTTASAADVQKYLTPGVGDT
                     AKGTITIDGSAQDVQISSDGKITASNGDKLYIDTTGRLTKNGSGASLTEASLSTLAAN
                     NTKATTIDIGGTSISFTGNSTTPDTITYSVTGAKVDQAAFDKAVSTSGNNVDFTTAGY
                     SVNGTTGAVTKGVDSVYVDNNEALTTSDTVDFYLQDDGSVTNGSGKAVYKDADGKLTT
                     DAETKAATTADPLKALDEAISSIDKFRSSLGAVQNRLDSAVTNLNNTTTNLSEAQSRI
                     QDADYATEVSNMSKAQIIQQAGNSVLAKANQVPQQVLSLLQG"
     gene            24669..26075
                     /gene="fliD"
                     /locus_tag="OO349_001864"
     CDS             24669..26075
                     /gene="fliD"
                     /locus_tag="OO349_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HCR5866957.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGDDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKAQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSSYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            26100..26510
                     /gene="fliS"
                     /locus_tag="OO349_001865"
     CDS             26100..26510
                     /gene="fliS"
                     /locus_tag="OO349_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HCR5866958.1"
                     /translation="MYAAKGTQAYAQIGVESTVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADVWKESLLSPSSIQDPV"
     gene            26510..26875
                     /gene="fliT"
                     /locus_tag="OO349_001866"
     CDS             26510..26875
                     /gene="fliT"
                     /locus_tag="OO349_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HCR5866959.1"
                     /translation="MNNAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLHLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            26953..28440
                     /gene="amyA"
                     /locus_tag="OO349_001867"
     CDS             26953..28440
                     /gene="amyA"
                     /locus_tag="OO349_001867"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HCR5866960.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIEHPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(28474..28887)
                     /locus_tag="OO349_001868"
     CDS             complement(28474..28887)
                     /locus_tag="OO349_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5866961.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRGIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            29074..30279
                     /gene="yedE"
                     /locus_tag="OO349_001869"
     CDS             29074..30279
                     /gene="yedE"
                     /locus_tag="OO349_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HCR5866962.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFPRE
                     APQTAKEIA"
     gene            30276..30509
                     /gene="yedF"
                     /locus_tag="OO349_001870"
     CDS             30276..30509
                     /gene="yedF"
                     /locus_tag="OO349_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HCR5866963.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            30616..31286
                     /locus_tag="OO349_001871"
                     /pseudo
     CDS             30616..31286
                     /locus_tag="OO349_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(31326..32534)
                     /locus_tag="OO349_001872"
                     /pseudo
     CDS             complement(31326..32534)
                     /locus_tag="OO349_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         8171 a         8334 c         7718 g         8407 t
ORIGIN      
        1 ttagcattag aatagttgcg ataagctgca ataagcagag ccaccttttt ggtttaataa
       61 gtccttacaa atagaagggg gtctttacac ttatctaaga tttttcctaa atcgacgcaa
      121 ctgtactcgt cactacacgc acatacaacg gaggggggct gcgattttca ataatgtgtg
      181 atgcagatca cacaaaacac tcaattactt aacataaatg tataagtcat acttttgttt
      241 tgggtgtatt tcaatctgtt aaaaagtttt tcgctacgct agcaagcaaa aatgaaacag
      301 gaataatcga aatgggatgt tgcgcacggt taaaataact caccgtaaat aatcatctgc
      361 tataaataat cactttcatg caataccaga taagctattt ttaaacagac acttaccgca
      421 caacaaacta attaaataca aagaaaggca acaatcaact taatgttaat aaaatgtttt
      481 tcatatatta accacttgtt tgatgcgggg aatcagtaca ttattttctt ataattacag
      541 ttgaaatcat atgatcaccg agagtgattg attaattgtc tttactaata atcagactaa
      601 tatttacctg tttgactgag ttgggattgc gtcgtttctc cattaggagt aaagctttaa
      661 tgtcacctga agttcacagc agaataagga acaggttatg agctatagca atattcttgt
      721 cgctgttgcg gttacaccgg aaagtcagca actgctggca aaagcggtat ctatcgccag
      781 gccagtaaag ggacacatca gtttaattac tctcgcttcc aacccggaaa tgtacaatca
      841 attagccgcg ccgatgcttg aagatttacg cagcgtgatg caagaagaaa cgcaaagctt
      901 tcttgataag ttaattcagg atgcggggta tcccgttgac aagacgttta ttgcctacgg
      961 tgagttaagc gaacatattc tggaagtatg tcacaaacat catttcaatt tggtgatttg
     1021 cggtaatcac aatcacagtt tcttttcgcg agcatcctgc tcggcgaaaa gagtgattgc
     1081 ctcaagtgag gtcgatgtgc tgttagttcc acttacggga gattaaccgc tcctacgcaa
     1141 gctttggaaa ggtagcaact ttatcgcgct gctggctttc cgcgcttcgc ggaactatct
     1201 gctttaggtc gctaatgaag cactcctgcc agtggttaat atcgtttttc acgataacgt
     1261 ccagcatttc tgcatgacgg gaaatacgtt ccaccagcga catagtcaat gcacgatcca
     1321 gcgcagctgc aacttcgtca cgatcgtagg ggttaacaat taacgccgac gttaactcgt
     1381 ttgccgctcc cgcaaattgc gaaagaacaa gaacgcccgg gttggctggg tcctgagcag
     1441 caacatactc ttttgctacc aggttcatcc cgtcacgcag tggcgtcact aagcccacgt
     1501 cagagtagcg gaatattttc atcagtaatt tacggtcaaa atgctgattc aaataataaa
     1561 gcggcgtcca gcctaattgc ccgtatttac cattaattcg tccagcttca ttttcgagct
     1621 gatgacgaat atcctggtag gcttgcacat caccacgcga cgttggtgca atctgggtat
     1681 aacgaatttt accatgatgc tgcggatatt tttccagcaa tgcctcatag gcgagaaaac
     1741 gctccggcaa acctttggaa taatccagcc gttcgacaga aaagatattt tgtacgtttt
     1801 tcaattccgc tttaagttgc gccagttttg gcggcagtgg cccggcagcc tgtttggcta
     1861 tttctttcgg ttcaatgccg atcgggtaga cttctgttcg aaatgctttt ccccaggctg
     1921 tatggctttt tgcgctacgt gtcgtgacgc gggtcaggtt agaaaggcaa tccaggaacg
     1981 ccagacgatc gttttctgtc tgtaaaccca gcaaatcata atcacaaagc tgttcaagca
     2041 aggtgtcata tgtcggcagc gcgttgaaaa tttccggtgt cgggaaagga atatgcaaga
     2101 aaaaaccaat gcgattattc accccacgtt tgcgtaattc atgcgcaaag ggcaacaggt
     2161 gataatcgtg gatccagata atgtcatcgt cttgcaacag cggcagtaat ttatctgcca
     2221 gcaacgcatt tacgcgtaga tagccgtccc aggcaggacg ctgaaattgc accagatcga
     2281 gccgataatg aaaagcgggc cagagaacgg cattggagaa ttggttgtag tattcgtcaa
     2341 ggtcctgttc gctgaggtta aaagaggccc acgtaatgtt acctttttta gcggctgatc
     2401 ctcattccct gtttcaccac tccagccaaa ccacagtccg cctgcggctt tcagtgcccc
     2461 cagtatgcca acggcaaggc caccggcact ggcggcgtgc tcgtctggtg gtgcaatccg
     2521 gttagatact acgactaaac gactcatagt catcactcct gttattttct cttttttgtt
     2581 gtaatgcggc ggttatcatt tcaagccagc tccagacatc cggcacaccc gccagtcgcc
     2641 atgatgcctg agttgcacct gtgccaattt ttactgacat tccgcccagt cggttaacga
     2701 ctgcgaagcc agattcatcg gttaaatcat cgcccagaaa tacgggcgtt cgcccgataa
     2761 aaggagcttc ctgcataaag gctgcaatcg cttcaccttt actggtacct ctcggtttga
     2821 tctcgacaac acactttccc tgctgtaacg ccatttgtgg ccagatctga gtaatacgtt
     2881 gcgctaatgt cattaatgcg tcttcatgct gcggagcctg acgataatgc agcgcaaaag
     2941 ccatcccttt cgcctccagc tccgcgccgg gatactgagc gatgaccgta tgcagttgca
     3001 cgctaatatc acgcgcaatc gcatccggca gatgaacgat atgtgtttta ccattgatgt
     3061 cacggcgctc cgccccatgc acgcccgcta acgggaagcg ataaggtttt gccagtgcgt
     3121 caagctccac cattgagcgc cctgatatca atgccaatgc accatcactt gcggttgcca
     3181 gtagctgtag tccttgcaga atattgtcag gcacgacgac ctgatcggga tgcggtttga
     3241 tttccgccag cgttccatca agatcaaaaa accaggcata tttcgcggat agttcagggg
     3301 tttcggttaa cggttctgtc acccggctct cctccttctc ttttcttatt gtgttgcgca
     3361 gaccgcgctt tacagacatg taagtataga cagtgtgacg gaggtcgcca tttgcaacga
     3421 agaacagcca accgttgtgg cggctggctg tggtgggaaa aaacgttaaa ttgttgtgga
     3481 aaaaagcatc agacagtgcg cttcgctttt tgcttgtaac ggtcgaagat caccgctgcc
     3541 agcaggatta agccgcgaac cacgtactgc gcgaaaggag aaatattaag caggttcatg
     3601 gcgttttcca cggtgcctaa aattaagata cccgccacca catatgagat ttttccgatg
     3661 ccacctttca gagaaacgcc acctaaaacg caggcggaga taacaataag ctcataacca
     3721 atcgacgtca ttggctgccc actagtcata cgtgaagcca gaataattcc ggctatcgca
     3781 gataccaggc cagagagaac aaagataata attttggtgc gaacaaccgg tatacccgcc
     3841 agacgcgcag cctcttcgtt cccgccaatc gccagggtat tacgaccaaa ggtggtttta
     3901 ttcagcagca aaccaaagat aatcagacac gcgacggtga gccagattgg cgcaggcaga
     3961 ccgaaccagt tagcgtaacc aagggcaaag aagctttcat cttcgatacc gaccgcttta
     4021 ccgtctgaaa tgatgtacgc cagacctcga acaatctgca tcgttgccag tgtcgtgatc
     4081 agagcattta ttttcagttt ggcgataaca aagccattga ccaggccaca gagaacgccc
     4141 agcaacaaac ccgctgccac gccaatccac aggctttcag tcaggttgat aaccaccgcc
     4201 gtggtgacac ccgcacaggc aattacggag gcgacagaaa ggtcaaagtc accggaagca
     4261 aggcagaaca acatgccaca cgccaccatc cccgacatgg aaattgccag gcccaaccct
     4321 ttcatattaa tgaaggtggc aaaatttggg acaaaaatgg cacaggcgat aaagagcacc
     4381 gcaaacacca ccagcatgcc gtactgatcc cagatacgcc cgaagctgaa tgacgactta
     4441 ggtgcgccag accccgatgt agaaacagaa gacatcatac tctccttact caggcaactg
     4501 cctggctgac tttaggcatc gcaaggctca gtgcctgacg ctcatctgcc tgctcgtgta
     4561 acaattcacc ggcgatttcg ccttcccgca tcaccacaat ccggtcggca acgccgagga
     4621 cttcaggtaa gtcgctggag gcaaacagca ccgccacgcc ctgcgccgcc agcgcataaa
     4681 ttacgttata tatttcgtgc ttagcgccaa catcaatgcc gcgcgtaggt tcatccagca
     4741 aaatgacctt catctcttcc gataaccagc ggcccagaat ggctttttgc tgatttccgc
     4801 ctgagagatt catgatcagt tgctccgcgc ccggcgtttt gatgttgagc gaacgaatgt
     4861 gctgatcggc attgttttct tcccaactgt tgttgattac acaaccgccg agcacatgtt
     4921 tgcgtctggc actgatgttg atattgtcgc gaacggagtg cacgggaata atgccttccg
     4981 ctttgcgagc ttccgggcag agcatcatgc ctgcggcaat ggcgtggctc ggtttacgaa
     5041 tatcgatcgg ctgttggtcg atataaacct gaccggcggt gatttgcgtc ccgccaaaca
     5101 agcctttcat taattcgcta cgccccgccc ctaccagacc aaacagccca acaatttcac
     5161 cactgcgaac cgccagactt attggcgtac gcacgcctag tgctttcaca gcatcaagac
     5221 gcaggcgctc ctcgccataa ctacgaggtt gccagccgta gatatcgcca atgtcgcgcc
     5281 cgaccatcgc ctgcaccagc gcgtcgtggt caacctgctg catatcggta aaggttttga
     5341 cataacgtcc atctttaaag acagtgatgg catcgctgag ggcaaatatt tcttccatac
     5401 ggtgagaaac gtataagatc acccgcccct cttttcgcag ttcacgaata acgcggaaaa
     5461 gattgccgat ttcgcgggca gaaagggagc tggttggctc atcaaaggcg ataattttag
     5521 cgttacgcgc cagcgctttg gcgatttcaa ccatctgcca ctgaccaatg gcgagatatt
     5581 tcagcggcgt gtccgggtca atatccatac caagatgttt aagttgtaaa cccgcctcat
     5641 aattcagcaa tgagcgattc acaatgccgc ctttatgcgg cagctggccg agatagatgt
     5701 tttccgcgac ggtcatttcc ggcacgagat gcagttcctg gtaaataatc gccacacccg
     5761 cgttaagtgc tgcggtcgtg tcggaaaagg acatttcctg cccattaatc actacagaac
     5821 ccgtggttgg cgcatagttg ccgctgagga tttttaagag agttgatttt cctgcgccat
     5881 tttcacccat caacgcatga acctgaccgg catagcagtc aaaactaata tccgtcagcg
     5941 ccttaacgcc gggaaacgtt ttaccgatgc cgcgaaatga gagatacggg gtagactgtt
     6001 gcataacgtc tccgtgaatc actggtcgtt cacaccccct ctgcatcatg cagaggggaa
     6061 tttttccggc aaattactta ccgcctaaac cttttttctc cagttcttct ttaaagttgt
     6121 cacgcgtgat cagtaccacg tctgtaactt cggtaaattt tggcgtttca acgtcttttg
     6181 ctacccagtt gtaaagcatt tcgctggatt tatagccatg tacgtccggg cttggcagca
     6241 gggaaccgta gaagccggtt gcctgtgctt tagacagttc gcttactgca tccacaccgt
     6301 taataccaat accgatgata tcggctgctt taaagccctg accttcggtt gcacgtacgc
     6361 cgccaagtac ggtgctgtcg ttcataccga cgatcagcca atgtttaact tccggatgtt
     6421 gaaccagcat tgagttggca gcgtcaaatg cccccgggat gtcgttagat ttggtaggta
     6481 cctgataaat ttgtttttcc gggaatcctg cagctttcag tgcgtccata gaacccgttg
     6541 tacggcggcg ggcggtatcc agttcgttgg cggtaatcgc catcactgcg ctttctttga
     6601 catcccagcc acgtttctgc atctctttat acagttccta gccctgacgt tcgccaattt
     6661 tagtcgccgc catcatcacc agaggaacgg tatccattgg cttacctttg gcgttaacaa
     6721 actggtcatc cactgcaatg actttcatat cgtagccacg cgctttcgcg acgatggcag
     6781 agccgagttt cgggtccgga gtacaaataa cgaaaccttt tgcgccactg gctgccaggc
     6841 tgtcgatcgc gttcaatgtt ttttcgccat ccggcacggc aatcttaata acctcaaacc
     6901 ctaaatcctt cccggcttta tcggcaaact tccattcggt ctggaaccac ggctcttccg
     6961 gttgcttcac cagaaaaccg agcttcaggt tctccgccat agcggattgt gacataacgg
     7021 ctgccagacc aatggctgcc agggctttag taaatttgtg catggttctc tccagcttta
     7081 gtgtcgtttt gtgtagtgca aaaacgaatg acattcgtta aattaatcgg aaaacaaagc
     7141 attacctttt atctaaaaga taagtgaccg tgttgacata gttttagcga gaaattaatt
     7201 ctccatagga gagcaatatc acatcgcaga attacagtga gaacgtgcat aaatttagcg
     7261 ggaaaagaca taaggaaaag ccaatttgtc agacaaattg ttgaatgcac agcagattaa
     7321 tccataagat tagcctggaa atccttgttg tcttcggtac ccattcggga tgtcttcttt
     7381 ttaaccagtc aataggccgc attacctggc gttgagtttt tgaaatggtg taataaccac
     7441 aactcaaaga tgtggaaaat gcgcgtcatt catttcgcta ttaattatca ctgtgctcat
     7501 taattaacag aacacgtata atgagagcca tctcgcaaaa atgaaaaaac gttttataaa
     7561 atcatcactt catcatgaat tcaaattcat cgattaatat caacaagata caaaaagcac
     7621 tatcattaaa attcattgca tttacattga tttcaccaat gaaatgtaaa aatatataaa
     7681 cttgatgatt taagcatttt cttataaccg ttcagacgtt attcttattt cagatcatcg
     7741 tcagaattga ctccacgatc acatttcgga ccggcagaaa ggaattattc tgcaaacagt
     7801 aattatggtg ttttgattta tcttgcacct ctccacttct ggatataagg atattaggta
     7861 tggcaaccgc tggaatgctt ctcaaactca actctcaaat gaaccgcgag ttttacgcat
     7921 ccaatctcta ccttcacctg agtaactggt gttctgaaca gagtctgaac ggcaccgcca
     7981 ctttccttcg cgcccaggca cagagtaatg tgacccaaat gatgcgcatg tttaacttta
     8041 tgaagagtgt cggcgctacc cccatcgtta aagccattga tgttccaggt gaaaaactga
     8101 actctctgga agaactgttc caaaaaacga tggaagaata cgagcaacgt tcaagcactc
     8161 tggcgcagtt agccgatgaa gcgaaagaac tgaatgatga ttcaaccgtc aatttcctgc
     8221 gcgatctgga aaaagaacag cagcatgatg gcctgttgct gcaaaccatt cttgatgaag
     8281 tgcgcagtgc gaaacttgcg ggtatgtgcc ctgtgcagac cgaccaacat gttctgaatg
     8341 tcgtgtcaca ccagctgcat tgatcataca tcggcgctaa tacattgcgc cgatgaagtt
     8401 tttgagaaac cgctgcctca tctgtttgaa gcagcggttt ttttaatggg attcaccctg
     8461 tggggtaaac ttgagttcaa tcagcgcgat ggctttttgg attgcccgca tggtgaccgg
     8521 gtctgcggca gcgggatgat gagtaaagtc gatattcttc agctgactgg acattttttc
     8581 acgaacttca acgggcgcga ttacatcgag aacatccaga atttgtttga taaccaactg
     8641 gcaagctacc acatcagaaa ccagttcctg atcggcattc agcggctggg acatcgtaaa
     8701 ctcctgatag cattttgaaa gccgttatag tagcggcttc acatcttcag cgatagtcac
     8761 atccaccgtc atcaggacac aaaaaaacct gccggagcag gttttttgtt atcggaacat
     8821 attgcctggc ggtacgtctt tgaacgtctt gcaatagtta ttgaacatac ttttcaggat
     8881 tttgcgcagt ttcatcgcgg cactccgacc atttgttata caggtgttat tgtctttgcg
     8941 cttataatat gacaaccatc acaaaaatca atctttatgt gatacaaatc acataaatac
     9001 ccctttaatg ttataaaaat gataatcaaa aaacagcccc cctatttctg acacctacag
     9061 atggcaagaa atagcgcctg ccaggcgtct tttccggcca ttgtcgcagc gctgtaacgc
     9121 gtaaaatagt gctttctctt actcttctgg ctggaccatg agacttctga ttctgactct
     9181 ttcattaata acgctggcgg ggtgtacggt tactcgccag gcccatgtga gcgaagttga
     9241 cgcggcaaca ggtattgtac ggttggttta tgaccaggct tttttgcagc atgcccatac
     9301 cgatcgttat gtcagtcgcg ggatcgccga tcgcgcatgc cagcaggaag gctataccca
     9361 cgcgatcccc tttggtcagc cagtaggcaa ctgtagcctt tttgcgggtt ccttatgttt
     9421 gaatacggaa ttcactttgt catatcagtg ccatcattcc gccttccctg tctttctcta
     9481 aatattttaa tcagcgaggg gatcttcgct gattaaagaa atagcggtaa tgcgttttat
     9541 tcccattcgt atttttaata attgaagttt atattttacc ttttgcaaat aataaaataa
     9601 caaattatag tgacgccaca gcaacaaata taacctttgt ggagcactat catgctgaaa
     9661 ccagaaatga ttgaaaaact taatgagcag atgaacctgg aactgtactc ttcactgctt
     9721 tatcagcaaa tgagcgcctg gtgcagctat cataccttcg aaggtgctgc cgcgttcctg
     9781 cgccgtcacg cccaggaaga gatgacgcat atgcagcgtc tgtttgatta cctgactgat
     9841 accggcaatt taccgcgtat taataccgtt gaatctccgt ttgctgaata ttcctcactt
     9901 gatgaattat tccaggaaac ctataaacac gaacaattaa tcacccagaa aattaacgaa
     9961 ctggctcatg ctgcaatgac caatcaggac tacccaacat ttaatttcct gcagtggtat
    10021 gtttctgagc agcatgaaga agagaaactg ttcaaatcga ttattgataa attaagcctg
    10081 gcaggcaaaa gcggcgaagg tctgtatttt atcgacaaag aactctctac cctcgacaca
    10141 caaaactaat gctctccggc ggcaggtttc tgccgccgtt tcaaatcagt gacggcaaat
    10201 cttactctga tcggtcgaaa aaccttcttc tgaaggtata aatttgcctt ttgctgccag
    10261 aaacgctacc agctctcccg ctgtcacccc ttctgccgag cacgtgtgaa aacgtgcctg
    10321 ttcaccaaaa cgcgctttaa tcgcagcttc cagactggca tgcgtatatt gctcgcctga
    10381 ttcaatcatc atatttaaca cttcatgacc gtgaatagag tccatcgttc ctcctcaaaa
    10441 aaagcctagc gtagcgatta ccgcttatga agactttgcg ccagcgcagg actgaatgct
    10501 tttattgtac atttatattt acacaatatg taacgtcggt ttgacgaagc agccgttatg
    10561 ccttaacctg cgccgcagat atcactcata aagatcgtca ggacagaaga aagcgtgaaa
    10621 aacagaaccc tgggaagtgt ttttatcgtg gcgggaacca caattggcgc aggcatgctg
    10681 gcaatgccgc tggctgcggc cggtgttggt tttagcgtta cgttaatctt gttgattggg
    10741 ctttgggcgt tgatgtgcta cacggcgcta ttactgctgg aggtgtacca gcatgttccg
    10801 gcagataccg gtctgggcac gctggcaaaa cgctatctgg gacgctacgg tcaatggctg
    10861 acgggcttca gtatgatgtt cttaatgtat gctctgactg cagcatacat cagcggtgcc
    10921 ggtgaattgt tggcctccag catcagcgac tggacaggta tttcaatgtc ggcaaccgct
    10981 ggcgtgctgt tgttcacttt tgttgccggt ggcgtggttt gtgttggaac atcgctggtc
    11041 gatttattta accgttttct gttcagcgcc aaaattattt ttctggtggt gatgctggta
    11101 ttgctgctac cgcatattca caaagtgaat cttttaaccc tgccgttgca acaggggctg
    11161 gcactgtctg caatcccggt gatttttacg tcgtttggtt ttcacggtag cgtgccgagt
    11221 attgtcagct atatggatgg caacattcgt aagctacgct gggtgtttat aatcggtagt
    11281 gccatccccc tggtggcata tattttctgg caggtggcga cgcttggcag cattgattca
    11341 acaaccttta tgggattgct ggctaatcat gctggattaa acgggctgtt acaggcgtta
    11401 cgcgaaatgg tggcctctcc gcatgttgag ctggcggtgc atttatttgc tgatttagcc
    11461 ctcgccacgt catttctcgg cgttgcgtta ggcttatttg attatctggc tgatttgttt
    11521 cagcgttcaa ataccgttgg tggacgattg caaactggtg caattacctt tctgccgccg
    11581 ttggcgtttg cactgtttta tccacgagga tttgtgatgg cgctgggtta cgccggtgtg
    11641 gcgctggcgg tactggcatt gattatccct tcactattga cctggcaaag cagaaagcac
    11701 aatcctcagg cgggttaccg ggtcaaaggt ggtcgtccgg cgctggtggt ggtgtttctc
    11761 tgtggtattg ctgtgattgg cgtgcaattt ttgattgcgg caggcttgtt accagaagtg
    11821 gggtgatcag atagcctcaa attccttatt gggtgccaga attaacgctg acacccaatg
    11881 tgacctctta atgcaggcag cactgcttaa atttcttacc actgccgcac ggacaaggat
    11941 cgttacgccc cggtttctct tctgctttga tcggttgctg aacagctttt tcctgcggat
    12001 gcgccatcca gtatgcatgt aaatcaagcg ccgccagtcg aatggcatct acgctctctt
    12061 caaacgcttc tggcgacatt ttctctaccc gctcgaagtt ttcctcagta ccgtgcagcg
    12121 caatcgcctc cagcgctggt tttaaagaat cgggcaacgt tgaccagtca gaaagtgcca
    12181 cgccccgcat atagccaaag caccactcct caacaatcgt cagctcgctg ccatcaactt
    12241 ctcgcaagcc gaataacggc tcaaactgct ccgggaattc gttcagacgc tctgcggtat
    12301 cggccatatg ttgaaaagcc aggttcataa agcgcgtcat ctctttctct gacgcccaac
    12361 gcggcacata gtcagcccca ccccacacgg caaccagcca ctgttctggt tcaatctctt
    12421 gcggagaact caacaccgcc gtcaataaac cgtccagctc cgccacatca aggatggcgt
    12481 ggtcagtgtt gtatttggtc agaatatcgt ccagccattc caactcactt tcgtttaacg
    12541 gtcccgtttt catacgcttt tccttgtgga tctcaactcg ccagcaccta tcttacatgc
    12601 cggtccgtat cagagatact ttttgagtgg ctttgccggt gattaaaaat taaggagggt
    12661 gtaacgacaa gttgcaggca caaaaaaacc acccgaaggt ggtttcacga cactgcttat
    12721 tactttgatt ttattcttat ctttcccatg gtacccggag cgggacttga acccgcacag
    12781 cgcgaacgcc gagggatttt aaatcccttg tgtctaccga ttccaccatc cgggctcggg
    12841 aagaaagtgg aggcgcgttc cggagtcgaa ccggactaga cggatttgca atccgctaca
    12901 taaccgcttt gttaacgcgc caaattcttc aggcctttca gccagacatc cgcttgacgc
    12961 cgatgtcttt taaactggag cgggaaacga gactcgaact cgagaccccg accttggcaa
    13021 ggtcgtgctc taccaactga gctattcccg cattcatcaa gcaatcagtt aatcacttga
    13081 ttttattatc gtctggcaat cagtgccgcc gttcgatgcg ttgcattcta cttacctggc
    13141 gcgatgagtc aacgatattt ttcaccactt ttgatcgttt gctgaaaatt acgccgaaac
    13201 gatcactgat caagcaaatc tgcacgcgca gcgctcaaat attgcaacat tgaccacaga
    13261 gtcagtaccg cagccacaaa gaaaagtgca ataccgacgt actcaaccca aatgttcgga
    13321 cgccacagca gccaggccaa cgccaccatc tgggcagtgg ttttcacttt cccaatccag
    13381 gagacggcca cgctactgcg tttacccaac tccgccatcc attcgcgtag cgcagaaata
    13441 ataatttcac gggcgatcat cgttgccgcc ggtaatgtca cccaccagct gtgataatgc
    13501 tcggttacca gcaccatggc gatagccacg agaactttat ctgccacagg gtcaaggaaa
    13561 gcaccaaacc gggtactctg gttccagcgg cgtgccagaa aaccatcgaa ccagtcagtc
    13621 accgccgcga cgcagaaaat gagcgcggcg gcaaacggcg accaggtgac aggcagataa
    13681 aagaccaata caaagaatgg gataaggatg acacggaaca gtgtaagcaa cgtagggata
    13741 ttaaattgca taatgacggg taactatctg ttgtcagtaa gattacccct atgttgctac
    13801 agagacatca atgtttcaac gaccagaaga tcttttctgc cagaccttgc gaaatacccg
    13861 gcacttttgc aatttcctcg acgctggcgt tacgtaaacc ttgcaaaccg cccatatatt
    13921 tcaacaacat ttgccgacgt tttggcccga cgccttcaat ggtttccagg gaactggtat
    13981 ttttgacctt cgcccgtttt ttacggtgcc cgccaatcgc gtgatcatgt gattcatcgc
    14041 gaatatgctg gataacatgc agcgcgggtg aatctggcgg caaactaaat ccctcacctt
    14101 ccggctcaaa gaacagcgtt tccagtccag ccttacgatc tgctcctttg gcaacgccaa
    14161 gtagcagcgg atgattttta tcccatgaga catccagttc ggcgaagaca tttttcgcct
    14221 gcgcaagctg gcctttgccg ccgtcgataa ggatcacatc cgggatctta ctgtcgtcaa
    14281 tggctttacc ataacgccga cgcagcacct gattcatcgc cgcataatca tcgcccggcg
    14341 tgatgcctgt aatgttatag cgccgatact ccgcacgcag cgggccgtta gcatcaaaca
    14401 ccacacagga agcgacggtt tgttcgccca tggtatggct gatgtcaaag cactccatcc
    14461 gcttcacttc cggcaatttc aacacgctgg caagcgcggt cagtcgctgg tgaacggtag
    14521 attgctgcga aagtttgctg gttaaggccg tcgccgcatt ggtgcgcgcg agtttcagat
    14581 aacgcgccct atcgccgcga ggtttggttt gaacattaat cttgcgtccc gccagttctg
    14641 aaagggaatc ggcgagcagc gttttatcgc taagattaaa atcgagcagg atctcacccg
    14701 gtaaggtgcg catctggctg ccttgtaaat agaactgacc tacgaaggtt tctaccacct
    14761 cgctcagttc cgtaccgcca ggcactttcg ggaaatagct gcggctgccg agcactttgc
    14821 cctgacgaat gaacaatacg tggacacaag ccatgcccgc atcgaacgcc acaccaataa
    14881 cgtcaaggtc gtcgccggta ttggaaacaa actgtttttc ggtgacgcgt cgcaccgcct
    14941 gaatttggtc gcgaatacat gccgcttctt caaactccag attctggctg gcagtttcca
    15001 tacggctaat gagttgcgta agcacctgat catctttgcc agacaaaaac aggcgcacat
    15061 actcgacctg ctgagcgtat tcttcttcac tcaccagtcc ttcaacgcac ggtcccagac
    15121 agcgccctat ctggtattgc agacacggac gcgagcgatt gcgataaaca ctattttcgc
    15181 actggcgaat ggggaaaatc ttttgcagta gcgccagtgt ttcacgtacg gcatagccat
    15241 tcgggaacgg gccgaaatat tcacctttgg catgctttgc accacgatgc atcgccagac
    15301 gcgggtgggt atcgccactc aggaagataa aaggataaga tttatcatcg cgtagcaaaa
    15361 cgttgtaacg cggctgatag agtttgatgt agttgtgttc cagcaacagc gcttcggttt
    15421 ctgtatgcgt aaccgttaca tcaatttgct ggatctgggc gaccagcgct tcggttttgc
    15481 gcgaagcgag gttgctgcgg aaatagctgg aaagccgttt tttcaggtct ttcgctttgc
    15541 cgacatagat aaccgtacca ccagcatcgt acatgcgata aacgcctggc tggctggtta
    15601 cggtttttaa aaacgctttt gcgtcaaact gatcactcac tgacttgata atgtctccgc
    15661 attacacaga ccatggcgaa ttgccaggtg agtcagctca acatcgccat gaatgtttag
    15721 tttactgaac atacgatagc ggtagctgtt caccgttttc ggactgagat tgagctgttc
    15781 tgagatctca ttgaccttct ggcccttagt gatcatcagc ataatctgca attcacgttc
    15841 agacaaactg gcaaatgggc tttctgtttt ttctggttcg atctggctta acgccatttg
    15901 ttgagcgata tcagaagcaa tgtaacgctg ccctgaatag acagaacgaa tcgcactcac
    15961 gacttcctgc ggagccgcgc ctttgctgag gtagccagca gcacctgcct gcatgacttt
    16021 cgctggtaaa gggttttctg tatggaccgt aagcatgatg attttaacat cagctgtgga
    16081 acgcgcgatt ttacgcgtcg cctcaagacc gccaatgccc ggcatactca tgtccattag
    16141 caccacgtca acggcatttg tccggcacca cttaacggcg tcttcaccgc acgatgcctc
    16201 tccggcgact tttataccct taatatcttc cagaatgcgt cgtatccctg cgcgcaccag
    16261 ttcgtggtca tcaacaagta gaacgttgat caaaggaata tctccagaaa tagggataac
    16321 gctactgata gttagtcatt cgtatattag cggtttttat tgcaactttg aaacgttaaa
    16381 aatggtgggt attcgatttt tctctcgttt ttgtcaattc gattgtccac agttatggtc
    16441 acgcccgtca ccaacggctt accggatcgt ttcacagtaa gccctacgat gaaatgtgac
    16501 aaaaattgac tttattcagc aaaaatgaaa atcagcccgc tgaatatgta acattaatta
    16561 acccgaacaa tacgggcaaa aaacattcat gataaaaata tttattggtc attatattaa
    16621 cgtattttat agcactgccg atatcacgct caaaaaacaa ccactgctat ttttagcaaa
    16681 gcttatggta tactccgccg ccttaacatt tttcaccaca aattttcatt gcaacatgac
    16741 gaggaaaata aatgagtacg cctgattttt ctactgccga gaataatcaa gaactggcaa
    16801 atgaagtctc ctgcctgaaa gcgatgctga cgctgatgct gcaggcgatg ggacaagctg
    16861 acgcgggccg cgtgatgtta aagatggaaa aacagcttgc gctgatcgaa gacgaaaccc
    16921 aggctgcagt attttccaaa acggttaagc aaattaaaca agcctaccgt cagtaatata
    16981 aaaccggctg agagcgtgcc tttcagccgg ttattgacaa aatcatcgct atctctgctg
    17041 atgtcttccc ttccgccgtc catttcagaa tttctttttc gcgcttgctg aaattcatct
    17101 ctggcgtcat cactatttca tcatttaaac gcatcagagc catcagactt tcgcgcacca
    17161 gtaactgcat ttttaattgc agttcatcac taagaatggg tatttcgcgc gtgctgcaac
    17221 gggaaaagga caaaaagccc agcgcccgat ttggcagcat taaatactga gtgacaccgc
    17281 ggcgtaaacc atgtgcgtgc gcggcttccc ataatggctg tgcttcgctg aataagtcat
    17341 cattccacat taaatggccc tgactaaagt tttcagggtt cagcacagga tcaattgcga
    17401 gaaaattttt tgcctgataa taactaaccc acgactcagg gtaattggtg tagaaagcca
    17461 ctttaggtcg cgtgaatggt accgggtggc gaacacataa cgaatagtaa tcgtactcca
    17521 gctgctgagc ctgaagctct atttcatggt aggcctcttc tgcggcctcc atcctctgaa
    17581 aacgcaacag catcgtgcga cgccagctga aaaaatccgt atcctgcata gtaaaccgca
    17641 acgcccctga gagtgagtat catttataat gataattcaa actatcacat aaataagatt
    17701 tatatataat ttatattatt caggcaatga attacttttg caagccatcg cattctctta
    17761 tgttattaat gagttatgct gatttgttaa gcagttttat cacgcttgaa atggcgtccc
    17821 gccccgacag gtgaatcgtc ggggctgatt ttttcttatt attgcagcag aagcttctcg
    17881 aggaactggc gggtgcgagg ctgctgggga tcggcaaata acgcttttgc tgccccctgc
    17941 tcgactatcc gcccctggtc cataaagatc gcccggtccg caacatcccg ggcaaagctc
    18001 atttcgtgtg tcacaatcac catcgtgcgc ttttcctgcg ccagctggcg gatggtgttc
    18061 aggacttcgc ccaccagctc tggatccagc gccgacgttg gctcgtcaaa caaaatcacc
    18121 tcaggacgca ttgccagtgc acgcgcaatc gcaacacgct gctgttgacc gccagacaaa
    18181 cgacgtggat agctggtttc tttaccggcc agcccaactt tcgccagcag ctcgcgagcg
    18241 cgcgccgtgg cctcttcttt cggttcgcct ttgacgatca ccggcccttc aataatgttt
    18301 tccagcaccg tacgatgcgg aaacaaatta aagttctgga agacaaaccc gacgtgctga
    18361 cgcaactggc gaatcagcga tttttgctga cttaatgaac gtgcagtatc aatagtgata
    18421 tcgccaacgg tgatcgttcc cgcttccggt tgttccagca gatttatgct acgtagcaat
    18481 gtggttttgc cggaaccact cggaccgata attgccacca cttcgccagg ctttacctcg
    18541 aggtcgatac cgtgcagcac cgtctgaccg tggaattttt tcaccaggtt cttaacttca
    18601 atggcactca ttttggttct ctctcctggc gattaagttg attctcaaaa tggttctgca
    18661 acgtcgataa cacagtcgcc atgatccagt agatgagcga agccgccagg tacatggtga
    18721 acacctccag cgtgcgcgag gtaatcaact gcgcctgacg gaacagctcc ggcacctgga
    18781 ttgtcgcggc cagcgacgta tcttttacca gactgataaa gctgttcgac agcggcggca
    18841 acgccacccg cgccgcctgt ggcaaaatcg cgcgacgcat ggtctgccac ggcgtcatac
    18901 caatactggc agcggcttcc cactgacctt tatcaataga agaaatggcg gcgcgcagcg
    18961 tttcggcggc ataggcggca gtgttcagcg acagaccaat cattgctgac ggaatgggat
    19021 ctaattcgat accaaactgc ggcaggccgt aatagatcat aaacagttgt gcgatgagcg
    19081 gcgtaccacg aaaaatagag atataaaaac gcgccagcca gcgcacgggc cagattggcg
    19141 acaggcgcat cagcgcgaga ataaacccga gtagtaagcc aaaaaacatg ccgccgatac
    19201 tgagttgcag ggtataccca gcccctttca acaggaacgg caaggaatca ataaccagtt
    19261 gtatactttc ttgcatgagc gttcccggag ctaaacgtgg ggatgatagg cgaacagcgc
    19321 aggcgcgccg ccggtatgaa taaacagaat cggcccttca tctttgaagc gtttctgact
    19381 gataccgtca atcagccccg ccatcgcttt tccggtatac acaggatcaa gcagaatgcc
    19441 ttccagacgc gccagcaatt tcactgcttc catgccttcg tcgttcggca cgccgtagcc
    19501 aggtgcaaaa tagtcatccc agagtaaaat ttccgctgat gcggtcagct ccagttcttt
    19561 cgcaatcgcc tgttgtaggt taaccacttt cggcaattga tcggcaacgg aacgcgacac
    19621 ggtcacgcca atcagttcgc tttcaggcat caggtgttcc agcccaacag ccagtccggc
    19681 gtgagttccg gcactgcccg atgcgactac caccgacgaa atattaaccg ccccttcaca
    19741 ctgttgcgcg atttccagcg cactctccac ataacctagc gcgccaagag cattagaacc
    19801 gccaaccgga atgacatacg ggcgaaagcc ttgtgcttcg actcgcgtcg ccagttcttc
    19861 cagttgggca ttgggatcgg tcagtgcgtc gcacatttca atctgggtat tgaacagatc
    19921 caacaacaaa cgattgccgt tggttaaata gttttctgcg gttgtgccaa taggattttc
    19981 cagcagcgcc acgcagtgca gaccgagttt cgccgcgact gcggcagtct ggcgcacatg
    20041 gttagactgg atcgcgccgg cagtaatcag cgtatcggca ccttcgcgca gggcatctgc
    20101 cgcgagaaat tccagcttac gtaatttatt gccgcccatt gccatggggg tgacctcatc
    20161 ccgtttgatg aaaatttccc gtccaagata atcagaaaag cgcggcagat attcgagcgg
    20221 cgttggcgcg ccgataaact ccaaccgtgg aaaacgggtt aaattatgca gtggcataac
    20281 agcctccgat gtgtgttgtt gtgattttct tattatgcac gctgaaaacg cgtaaataaa
    20341 aaaggcgcta gtgaaagcgc ccttttttgt cattatgctg attatttggt cacatcagca
    20401 ccaaaccatt tttcggaaag agcttgcaga gtgccgtctt tttgcatttc cgcaattgca
    20461 tcattcaccg ctttcagcag gtcttcattt cctttacgca gcgccacgcc agactcctga
    20521 cgggagaatg cttcaccggt tactgccagc gtatcgttgg ttttcttcac cagatccagc
    20581 gccgccagac gatcaacgag gatcgcatcg atacgcccta cgcgcagatc ctgatatttg
    20641 gtcgggtcat catcataggt acgcacatcg acaccctgaa cattctgccg cagccactct
    20701 tcatagttgg tgcccagacc gacacccact tttttgcctt tcagatcagc agccgtttta
    20761 atggtgcctt cgttaccttt tttcaccagc gcctgaatac cggaaatggt gtaaggggta
    20821 gagaaatcgt attttttctt gcgctcgtca gaaatggtga cctgattaat caccacatca
    20881 atgcgtttag agtccagcga cgccagcata ccgtcccatt tggtcggttt tagtgacgcc
    20941 tcaacgccaa gatgttttgc cagctgttgg gcaaattcca cttcaaaacc ggttaatttg
    21001 ccgtcatccc cctgaaaact gaacggcgga taagtgcctt ccagccctac cagcagcgtg
    21061 ccgctctctt tgactttatt aagcagacct tcatctgcaa aacttttaac gctcatgccc
    21121 gcaaccagcg ccacggccat cacacccatc aatgcctgac gtcccagatg tgctaatttc
    21181 atattcaccc cgaatgttgt tatgtctgtt tgcagtgtag agccattgtt tgtaaacaca
    21241 aaaacaactc cgctacatct tattcttatt taatatatat cagaagcagg caggctggag
    21301 gatttctgca caagtccggt acaggtttgg cgctgatagt gttgatactt ccgtaatgcg
    21361 atgcggtaat tgttggtgct ggtggtgggc agccacgggg cgaggatttc atcaaggaaa
    21421 ccgtcctgca gctgatcgag ggaaatattt tgctcatgta gatgattacc caaacggcgc
    21481 agacgaacga catattcacg caccgtacct gggctcattt cagtttgctc aaaaagaaat
    21541 ttcttaaagc cgataatatc gaaaaaatca ctctgcgttt tgcaatgtaa atcaccgcaa
    21601 aatcggcaca atgccgccca tgattgttgt tccgtttgcc agcctttttc atcaagcagc
    21661 gtgtccaggc gggaaatctc gattttattc actattttgc cgtcgcgaac taaggtaatg
    21721 cgatcgagta atttacggca atgcgcgcaa tgggtctggc tgtgtttaaa gtctttaaga
    21781 tagcggctta atggccgtct tttcaggtgc tgcaccatca ttaagaactc ctggtagtca
    21841 aagttaaagt gcggcattta ctgacgttat aacttaccca gtttagtgcg taaccgttta
    21901 atggcctggc tgtgtaactg actgacccgc gattccccga cctccagcac cgcgccaatc
    21961 tctttgagat tcagctcttc ctggtaatag agggttaata ccagtttttc gcgctccggc
    22021 aacgtttcga tggcttccat cacccgctgg cgcagattac tgtccagcag ttgttgtagc
    22081 gggttttctc gctgatgatc atcagtaacc agttcgatgc tatcgccgtg ctcttcacgc
    22141 cactcatcgt aggagaagag ctggctgtta ttggtgtcga gcagcatttg acgataatcg
    22201 gcaatatcga tccctaaacg ttccgctacc tcagtttccg tggcgttgcg gccaagttcc
    22261 tgctccagtt gccctattgc ctgtgccact tcacgcgcgt tgcgtcgcac gctgcgcggc
    22321 acccagtcac ggctgcgaag ttcatccagc atagcgccac ggatacgctg cactgcgtaa
    22381 gttgtaaatg ccgttccttg tagggcgtca tagcgttcga cagcattaag taacccaatg
    22441 ccgcccgcct gtagcagatc gtcaagttcc acgctcgcgg gcagtcgaac ctgcaggcgc
    22501 aatgcttcgt gacgcaccag cgggacataa cgctgccaca gcgagtgttt atccattaca
    22561 ccttcagcgg tatagagtga attcacgata aacagccctg cgttatatga gttatcggca
    22621 tgattatccg tttctgcagg gtttttaatc ggacgattag tgggtgaaat gaggggttat
    22681 ttgggggtta cgcaaaaagc cgaatgtaaa aaaccccgcc ggtggcgggg tttgagaggc
    22741 attcagacga ttaaccctgc agcagagaca gaacctgctg cggtacctgg ttggcttttg
    22801 ccagcacgga gttaccggcc tgctggataa tctgcgcttt cgacatgttg gacacttcgg
    22861 tcgcatagtc ggcgtcctga atacgggact gcgcttcaga caggttggta gtggtgttgt
    22921 tcaggttggt gactgcggaa tccaaacggt tttgcaccgc acccagggag gagcggaatt
    22981 tgtcgatgga gctgatggct tcatccagag ctttcagggg atcggctgtc gtagctgctt
    23041 ttgtttcagc atctgtcgtc aatttaccat cagcatcttt ataaactgcc ttaccgctgc
    23101 cgttagtcac tgaaccatca tcctgtaggt aaaaatctac agtatcagat gtggtcaacg
    23161 cctcgttgtt atcaacataa accgaatcaa caccttttgt tacagcgcca gttgtgccgt
    23221 tgacgctata acctgcagta gtgaaatcaa cattgtttcc cgaggttgat acagctttat
    23281 cgaaagctgc ctgatcaact tttgcacctg ttactgaata agtaatagtg tccggcgtag
    23341 tactattacc ggtaaaggag atagaggtac cgccaatgtc aatggttgtc gctttggtat
    23401 tattggctgc aagtgtggac agactagcct cagtcaaaga agcaccagag ccgtttttcg
    23461 ttaagcgccc agttgtatca atgtagagtt tatctccatt gcttgccgta attttaccat
    23521 cactgctgat ctgaacatcc tgtgcagaac catcgatagt aatagtgccc ttagcggtat
    23581 caccaactcc cggggtcaaa tatttctgaa catcggcagc tgaagccgtt gtggtatcaa
    23641 aagagtatga cttacttgca ctgtcatact tgtaattcgt tgcactagcc gccgttccga
    23701 agccgttatt aatggttgcc tgaacagtat caccatcact aactccagcc agcaaatcag
    23761 acgctttagc agcatcgtaa cccgcactca cagtatattt gttgccgacc actgttgcat
    23821 tagcagctac caagtcagct ttagatgcag cagtattagc cacagccccg ccaccattca
    23881 cattaaaccc attcagcccc agcgtatcag agtcaatttt cttcagatca atagtgatag
    23941 tctggccgtc attcgcacca acctgaattt tcatcgaacc gtcttttgcc agtacgttca
    24001 cgccgttgaa ctgggtctga ccggatacgc ggtcaatttc gtcgagacgg gatttgattt
    24061 cgtcctgaat ggagtccaga tccgaatcag agttagtccc ggtagaagcc tgaaccgtca
    24121 gctcacggat acgctgtaag ttgttgttga tttcggacag cgcgccttca gtggtctgtg
    24181 caacagaaat accgtcgttg gcgttacgtg cagcctgagt caggccttta atgttagaag
    24241 taaaacggtt agcaatcgcc tgacccgcgg cgtcatcctt cgcgctgtta atacgcaagc
    24301 cagaagacag acgctcgata gaactcgaca gcgcagactg gttcttgttg atattatttt
    24361 gagtgatcag cgagaggctg ttggtattaa tgacttgtgc catgattcgt tatcctatat
    24421 tgcaagtcgt tgattacgtt ttgggtttct acccgtcggc tcaatcgccg tcaaccctgt
    24481 tatcgtctgt cgtaaaacaa cctttagaat ttttttcaca aacagccatt ttttgttagt
    24541 cgacgaaata ctcttttctc tgccccttat tcccgctatt aaaaaaaaca attaaacgta
    24601 aactttgcgc aattcaggcc gataaccccg gtattcgttt tacgtgtcga aagataaaag
    24661 gaaatcgcat ggcaagtatt tcatcgctgg gagtcgggtc aggtctggat ttaagttcaa
    24721 tccttgatag cctcaccgcc gcgcaaaaag cgacgctaac ccctatttca aatcagcaat
    24781 cgtcgtttac cgctaaactt agcgcctacg gtacgctgaa aagcgcgctg acgactttcc
    24841 agaccgccaa tactgcattg tctaaagccg atcttttttc cgccaccagc accaccagca
    24901 gcaccaccgc gttcagtgcc accaccgcgg gtaacgccat cgccgggaaa tacaccatca
    24961 gcgtcaccca tctggcgcag gcgcaaaccc tgaccacgcg caccaccaga gatgatacga
    25021 aaacggcgat cgccaccagc gacagcaaac tcaccattca acaaggcgac gacaaagatc
    25081 cgattaccat tgatatcagc gcggctaact catcgttaag cgggatccgt gatgccatca
    25141 acaacgcaaa agcgggcgta agtgcgagca tcattaacgt gggtaacggt gaatatcgtc
    25201 tgtcagtcac atcaaatgac accggccttg ataatgcgat gacactctcg gtcagcggtg
    25261 atgatgcgct acaaagtttt atgggctatg acgccagtgc cagcagcaac ggtatggagg
    25321 tctcggtcgc cgcccagaat gcgcagctga cggtcaacaa cgtcgccatc gagaacagca
    25381 gcaacactat cagcgacgcg ctggaaaaca tcaccctgaa cctgaacgat gtcaccacgg
    25441 gcaaccagac gctaaccatc actcaggaca cctccaaagc gcaaacggcg attaaagact
    25501 gggtgaatgc ctataactcg ctaatagata ccttcagcag cctgaccaaa tacaccgccg
    25561 tagatgcggg agctgatagc cagagttcta gcaatggcgc actgctcggc gactccacgc
    25621 tgcggacgat tcagacgcag ttgaagtcga tgctgagtaa taccgtcagt tcttccagct
    25681 ataaaacgct ggcgcagatt ggtatcacga ccgatcccag cgatggcaaa ctggaactgg
    25741 atgccgacaa actcaccgct gcactgaaaa aagatgccag cggcgtaggt gcattgattg
    25801 ttggcgatgg taaaaaaacc ggcatcacaa ccaccatcgg cagcaacctg accagttggc
    25861 tttcgacaac gggcattatt aaagccgcta ccgatggcgt tagtaaaacc ctgaataagt
    25921 taactaaaga ctacaacgcc gccagcgatc gcattgatgc gcaggtcgcg cgctacaaag
    25981 aacaatttac ccaactggac gttttaatga cctcgttaaa cagcaccagc agctacttaa
    26041 cgcagcagtt cgaaaacaac agtaattcca agtaatcatt attcatcggg agacaggtca
    26101 tgtacgcggc aaaaggcacc caggcctatg cacaaattgg cgtcgaaagc accgtaatga
    26161 gcgccagcca acagcagttg gtcaccatgc tatttgatgg agtgctgagc gcactggtta
    26221 gagcgcgact gtttatgcag gacaacaatc agcaaggcaa aggcgtctct ttgtcaaaag
    26281 cgatcaacat cattgagaac ggactgcggg tgagtcttga tgaagagagc aaagacgaat
    26341 taacccaaaa cttaattgct ctttatagct atatggtcag gcgtttgctg caagccaatt
    26401 tacgcaacga tgtctccgca gtcgaagagg tggaagcatt aatgcgtaat attgccgatg
    26461 tctggaaaga gtctttactc tccccttctt cgattcagga cccagtctga tgaacaatgc
    26521 accgcatttg tatttcgcct ggcaacaact cgtcgaaaaa agccagctca tgttacgcct
    26581 ggcaacggaa gaacaatggg acgaactcat cgctagcgaa atggcgtatg tgaatgcggt
    26641 gcaggagatc gcacacctga ctgaagaggt tgccccgtcc actacgatgc aggagcagct
    26701 ccgcccgatg ctgcacctga ttctcgacaa cgaaagcaag gtaaagcagt tattacagat
    26761 tcggatggat gaactggcga aactggtcgg tcagtcatcg gtgcaaaaat cggtgttaag
    26821 tgcctatggc gatcagggcg gctttgtgct ggctccgcag gataacctct tttgaatctt
    26881 aatgagccga tggctcgcga ataatccgat tacggctacg cttctaatat tccccttgaa
    26941 tggagtcgaa gaatgcgtaa tcccacgctg ttacaatgtt ttcactggta ttacccggaa
    27001 ggcggtaagc tctggcctga actggccgag cgcgcagacg gttttaatga tattggtatc
    27061 aatatggtct ggttgccgcc cgcctataaa ggcgcatcgg gcggctattc ggtcggctac
    27121 gactcctatg atttatttga tctaggtgag tttgatcaga aaggcagcat ccctactaaa
    27181 tatggtgata aagcacaact gctggccgcc attgacgccc tgaaacgtaa tgacattgcg
    27241 gtgctgctgg acgtggtagt caaccacaaa atgggcgcgg atgaaaaaga agctattcgc
    27301 gtgcagcgtg taaatgctga tgaccgtacg caaattgacg aagaaatcat tgagtgtgaa
    27361 ggctggacgc gttacacctt ccccgctcgt gccgggcaat actcgcagtt tatctgggat
    27421 ttcaaatgtt ttagcggtat cgaccatatc gaacaccctg atgaagatgg catttttaaa
    27481 attgttaacg actataccgg agaaggctgg aacgatcagg ttgatgatga actcggtaat
    27541 ttcgattatc tgatgggtga gaatatcgat tttcgcaatc atgccgtgac ggaagagatt
    27601 aaatactggg cgcgctgggt gatggaacaa acgcaatgcg acggctttcg tcttgatgcg
    27661 gtcaaacata ttccggcatg gttttataaa gagtggatcg aacacgtaca ggaagttgcg
    27721 ccaaagccgc tgtttattgt ggctgaatac tggtcgcatg aagtcgataa actgcaaacg
    27781 tatattgatc aggtggaagg caaaaccatg ctgtttgatg cgccgctgca gatgaaattc
    27841 catgaagcat cgcgcatggg tcgcgactac gacatgacgc agatcttcac tgggacgctg
    27901 gtggaagccg atccttttca cgccgtaacc ctcgttgcca accacgacac ccaaccgttg
    27961 caagccctcg aagcaccggt cgaaccgtgg tttaaaccgc tggcgtatgc cttaattttg
    28021 ttgcgggaaa atggcgttcc ttcggtgttc tatccggacc tctacggtgc acattacgaa
    28081 gatgtcggtg gtgacgggca aacctatccg atagatatgc caataatcga acagcttgat
    28141 gagttaattc tcgcccgtca gcgtttcgcc cacggtgtac agacattatt tttcgaccat
    28201 ccgaactgca ttgcctttag ccgcagtggc accgacgaat atcccggctg cgtggtggtc
    28261 atgtcgaacg gagatgatgg cgaaaaaacc attcatctgg gagagaatta cggcaataaa
    28321 acctggcgtg attttctggg gaaccggcaa gagagcgtag tgacagacga aaacggcgaa
    28381 gcgaccttct tttgcaacgg cggcagcgtc agcgtgtggg ttatcgaaga ggtgatttaa
    28441 atccatcccc ggcggcaagc cggggagatt tcattacggc agtggcgtcg gtaacggcgc
    28501 tttatccagc gcggcggcac actctgccgt cgggcgatcc acacgctcca tcgtcatacc
    28561 atcgtactca atggtgttac cgtcacgctc aacctcgtac agctcacgtt ttacggtaac
    28621 gttagtgaga tcatcagaca tcaatgtcaa cttacccggc acggcaatcc cccgctgcca
    28681 ctgacggcaa tccaaggtat cgccctcttt agtgacaatc aggctgccta tcgcttccgg
    28741 actgaccagc gcacgttgcg gccctttggt ctgccagtag cccgccagcc agtccggcgc
    28801 gggggttttc acgacattgt tatagttttc gacttcggca cagcccgcca gcaacatgag
    28861 cgcgcctgca attgcgagtt ttttcatcat tcgtcctgca tgagaagaaa agaggattgt
    28921 ggcattaaag ctctgatgtc gccagccttt ccaggttcag agagaaattt gatctaacta
    28981 ttatttttgt gaattattac cccgaaaata aatttgcatg gtgtatgatt cgcggctctt
    29041 gtttcaaacc ttctgagttc agaggctaca ttcatgtcat ggcagcaatt caaacacgcc
    29101 tggttgatta aattctgggc acccatccct gcggtcatcg cggcgggtat tctctccact
    29161 tactattttg gcattactgg caccttttgg gctgtcacgg gtgaatttac ccgttggggc
    29221 ggtcagctcc tgcagctgtt tggcgtccat gctgaagagt ggggttattt taaaattatc
    29281 catctggaag gatcgccatt aacccgcatc gacggcatga tgatcctcgg tatgtttggc
    29341 ggctgctttg ctgctgcgct gtgggccaac aatgtcaaac tacgcatgcc acgcagccgt
    29401 atccgcatta tgcaggccat cattggcggc attatcgccg gttttggcgc gcgtctggca
    29461 atgggctgta acctggcggc gttctttacc ggcattcctc agttctcgct gcatgcctgg
    29521 ttctttgcca tcgccactgc cattggttca tggtttggcg cgcgctttac cctgctgccc
    29581 atcttccgta ttcccgtgaa aatgcagaaa gtttctgccg cctcaccgct gacgcaaaaa
    29641 ccggatcagg cgcggcgtcg ttttcgtctc gggatgctgg tctttttcgg catgctgggc
    29701 tgggcgctgc tcacggcgat gaaccaacca aaactggggc tggcaatgct gtttggcgtc
    29761 ggctttggtt tactgattga acgtgcgcaa atctgcttta cttcggcgtt ccgcgatatg
    29821 tggatcaccg gacgtaccca tatggcgaaa gcaatcatta tcggtatggc ggtgagtgcc
    29881 atcgggatct tcagttacgt acagttaggc gttgaaccta aaatcatgtg ggcgggacca
    29941 aatgcggtaa ttggtggttt actttttggt tttggcatcg tgctggcagg cggctgcgaa
    30001 accggctgga tgtaccgcgc ggtagaaggc caggtgcact actggtgggt cggtctgggc
    30061 aacgtgatcg gctcaacgat tctggcgtat tactgggatg atttcgctcc ggcgctggcc
    30121 accgactggg acaaaatcaa cctgctgaaa acttttggtc cgatgggagg cctgctggtg
    30181 acatatttgc tgctgtttgc tgcgctgatg ttgattatcg gctgggaaaa acgcttcttc
    30241 ccccgtgagg caccgcagac tgctaaggag atcgcatgaa aaatatcgtt cctgattacc
    30301 gccttgatat ggtgggtgaa ccctgccctt atccggcagt cgcaaccctt gaggcgatgc
    30361 cgcaattaaa aaaaggtgag atcctggaag tggtgagcga ctgtccgcag tcgatcaata
    30421 atattccact ggatgcgcgt aatcacggct acacggtgct ggatattcag caagacgggc
    30481 cgaccattcg ttatttaatt cagaagtaat ccctcattcc cgctgtggcc cacagcggga
    30541 acatcgttac ctctgacaca tagattttac ccgccccctc tcctattctt ttcagtatga
    30601 tctcaggagg ttgctatgtg tggacgcttt gcccaatccc aaacgcgtga ggattacctt
    30661 gcgcttctcg cggaagatat tgaacgcgat attccctacg atcccgaacc cattggcaga
    30721 tacaatgtcg cgccgggaac caaagttctt ctgctcagtg aacgtgatga acaccttcat
    30781 ctggatccgg ttttctgggg atatgctccc ggatggtggg ataaaccgcc gctgattaac
    30841 gcccgcgtag aaactgcctc aaccagtcgc atgtttaaac cgctctggca acatggtcgg
    30901 gcaatctgtt ttgccgatgg ctggtttgag tggaaaaaag aaggcgacaa aaaaagcctt
    30961 attttatcta tcgcgctgac ggacaacctg tttttatggc cgcgataggt agcataccgt
    31021 ttgagcgtgg tgacgaagcc gaaggatttt tgatcgtcac tgcggcggca gatcaaggtc
    31081 tggtagatat tcatgaccga cgcccgctgg ttctgtcgcc agaagctgca cgggaatgga
    31141 tgcggcaaga cattggtggt aaagaagcct cagaaatcgc taccagaagc tgtgtgccag
    31201 caaaccagtt cacctggcac cctgtgtcgc gcacggtggg taatgtcaaa aaccagggcg
    31261 cggagttaat tcaacctgtt tgttgacatc ctccacgccc tgaatgacga ggacccctgc
    31321 tacgttcagg ctgtcgcctg aatcatttcg gtgggttcct gcttcaacga gcggcctgac
    31381 tgcaccatct ctccacaggc aagaacggcg tgccccgccg ctaaaatgtt acgagctcca
    31441 tttacatcgg cgttcgctgt atatccacat acctggcatc tgaatttact ttgtgacagg
    31501 cgattttctt tcgctgtatg accacagcac gcgcaacgct ggcttgtgta cgctggcgga
    31561 acagcaagca cctgaccgcc acgccagagc tgcttgtact caagctggcg gcgcatttca
    31621 taccagccct gatccagtat cgaacggttt aaacctgatt ttgcccggac attgcgcccc
    31681 ggctggctga ccgtacccgc tgctgacttt gacatgttgc tgaccttcaa atcctcaatg
    31741 actatcatta cgtggttttt gctgacggtc gttgtgactt tgtgaaggta gtccctgcgg
    31801 atatttgcga tacaggaatg cagtcgctgt attttgcgtt tctgcttctg ccagttgttg
    31861 ctgaatttga ccttgcggct taactgtcgc tgaagtctcg ccagcttctt ctggtttttc
    31921 tgaaaactgt ttacaggctc aaagactgtg ccatctgaca gcgaggcaag tttagccacg
    31981 ccagcatcca gcccgaccat tgatgctgaa gggtgaaccg gagttgatac ttcactttct
    32041 gcctgaatac tgatgtacca cttaccgcag gactggctga cagtgacatt tttcacaaca
    32101 cccgtgacct gacggctgtt ccggtagcgc atccagccaa gttttggcag aaaaatacgg
    32161 ctgttttcct gagcgagctt aacgccctgc ggatagcgga atgcatcatt ctgtcaccgc
    32221 tttttgaatc aggggaacgc cgagcgcttc tggaaaaagt ttttgcaggc ctgctcaggg
    32281 tctttcagtg actgttgcaa tggctgtgac ggagcatctt taatccattg cgtttcagtg
    32341 gcatttttcc actcaaccaa tcaggaagcc attttcgcgt aagggatgtc tttgttaccg
    32401 gccttatgat tctcattctg acgtgccaga gtacgactga aaacgaaacg acatgcgcct
    32461 gcgaagcgcc tcatttcacg cgcctgttga ccaccgggtc ttaaccggaa tttgaatacc
    32521 tgtagtcgtt tcacagggca aattatactt tggtctgtga aaaaaactga tatccgacgc
    32581 ggaaggcact gtgttttcct gatgcatgct caccgggtct ttgttaccaa
//
LOCUS       DAJTQY010000038        32564 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.038, whole genome shotgun sequence.
ACCESSION   DAJTQY010000038 DAJTQY010000000
VERSION     DAJTQY010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32564
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.038"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(<1..956)
                     /locus_tag="OO349_001873"
     CDS             complement(<1..956)
                     /locus_tag="OO349_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HCR5866964.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKIEVNLGAGEAGS"
     gene            1202..1828
                     /gene="gstB"
                     /locus_tag="OO349_001874"
     CDS             1202..1828
                     /gene="gstB"
                     /locus_tag="OO349_001874"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HCR5866965.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(1825..2939)
                     /gene="yliI"
                     /locus_tag="OO349_001875"
                     /pseudo
     CDS             complement(1825..2939)
                     /gene="yliI"
                     /locus_tag="OO349_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
     gene            complement(3050..3433)
                     /gene="bssR"
                     /locus_tag="OO349_001876"
     CDS             complement(3050..3433)
                     /gene="bssR"
                     /locus_tag="OO349_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HCR5866966.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            3646..4971
                     /gene="rimO"
                     /locus_tag="OO349_001877"
     CDS             3646..4971
                     /gene="rimO"
                     /locus_tag="OO349_001877"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HCR5866967.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ANMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(5018..6346)
                     /locus_tag="OO349_001878"
     CDS             complement(5018..6346)
                     /locus_tag="OO349_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCR5866968.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLFGLE
                     RFSLLSKSLVMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVILGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(6354..8702)
                     /locus_tag="OO349_001879"
     CDS             complement(6354..8702)
                     /locus_tag="OO349_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCR5866969.1"
                     /translation="MLSLYEKIKIRLIILFLLAALSFIGLFFMINYQLVSERAVKRAD
                     SRFELIQKNVGYFFKDIERSALTLKDSLYLLKNTEEIQRAVILKMEMMPFLDSVGLVL
                     DDNKYYLFSRRANDKIVVYHQEQVNGPLVDESGRVIFADFNPSKRPWSVASDDSNNSW
                     NPAYNCFDRPGKKCISFTLRINGKDHDLLAVDKIHVDLNWRYLNEYLDQISANDEVLF
                     LKQGHEIIAKNQLAREKLIIYNSEGNYNIIDSVDTEYIAKTSAVPNNALFEIYFYYPG
                     GNLLNASDKLFYLPFAFIIIVLLVVYFMTTRVFRRQFSEMTELVNTLAFLPDSTDQIE
                     ALKIREGDAKEIISIKNSIAEMKDAEIERSNKLLSLISYDQESGFIKNMAIIESNNNQ
                     YLAVGIIKLCGLEAVEAVFGVDERNKIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCR
                     ENVQTFTRKIAMVNDFDSSFGYRNLRIHKSAICEPLQGENAWSYAEKLKLAISSIRDH
                     MFSEFIFCDDAKLNEIEENIWIARNIRHAMEIGELFLVYQPIVDINTRAILSAEALCR
                     WVSAERGIISPLKFITIAEDIGFINELGYQIIKTAMGEFRHFSQRASLKDDFLLHINV
                     SPWQLNEPHFHERFTTIMKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGVRI
                     SIDDFGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAE
                     GVESEDVARELIKSGCVQAQGYLYQKPMPFSAWDKSGKLVKE"
     gene            complement(8880..9791)
                     /gene="gsiD"
                     /locus_tag="OO349_001880"
     CDS             complement(8880..9791)
                     /gene="gsiD"
                     /locus_tag="OO349_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HCR5866970.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGVAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(9794..10714)
                     /gene="gsiC"
                     /locus_tag="OO349_001881"
     CDS             complement(9794..10714)
                     /gene="gsiC"
                     /locus_tag="OO349_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HCR5866971.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMFLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(10732..12270)
                     /gene="gsiB"
                     /locus_tag="OO349_001882"
     CDS             complement(10732..12270)
                     /gene="gsiB"
                     /locus_tag="OO349_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HCR5866972.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPTALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(12290..14161)
                     /gene="gsiA"
                     /locus_tag="OO349_001883"
     CDS             complement(12290..14161)
                     /gene="gsiA"
                     /locus_tag="OO349_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HCR5866973.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLRRRSRDVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYSHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGSRCAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(14148..15113)
                     /gene="iaaA"
                     /locus_tag="OO349_001884"
     CDS             complement(14148..15113)
                     /gene="iaaA"
                     /locus_tag="OO349_001884"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCR5866974.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLLAAREEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            15317..16552
                     /gene="moeA"
                     /locus_tag="OO349_001885"
     CDS             15317..16552
                     /gene="moeA"
                     /locus_tag="OO349_001885"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HCR5866975.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTTQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKMILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            16552..17301
                     /gene="moeB"
                     /locus_tag="OO349_001886"
     CDS             16552..17301
                     /gene="moeB"
                     /locus_tag="OO349_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HCR5866976.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(17480..18142)
                     /gene="fsa"
                     /locus_tag="OO349_001887"
     CDS             complement(17480..18142)
                     /gene="fsa"
                     /locus_tag="OO349_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCR5866977.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDALKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVTKFEQDWQGAFGRT
                     SI"
     gene            18273..19172
                     /locus_tag="OO349_001888"
     CDS             18273..19172
                     /locus_tag="OO349_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HCR5866978.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQACHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            19178..21610
                     /locus_tag="OO349_001889"
     CDS             19178..21610
                     /locus_tag="OO349_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HCR5866979.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALARAMAATETRESRRDELLAMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVHVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            21756..22571
                     /gene="ybiV"
                     /locus_tag="OO349_001890"
     CDS             21756..22571
                     /gene="ybiV"
                     /locus_tag="OO349_001890"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HCR5866980.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            22723..23988
                     /locus_tag="OO349_001891"
     CDS             22723..23988
                     /locus_tag="OO349_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HCR5866981.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLNRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRLPPGTTSKGLGAHTDSGALERWLLPAYQ
                     HVFANVFNGNLAKYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(24229..25821)
                     /locus_tag="OO349_001892"
     CDS             complement(24229..25821)
                     /locus_tag="OO349_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HCR5866982.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFAEPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            26040..26960
                     /gene="ldtB"
                     /locus_tag="OO349_001893"
     CDS             26040..26960
                     /gene="ldtB"
                     /locus_tag="OO349_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR5866983.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVEHKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(27018..28136)
                     /locus_tag="OO349_001894"
     CDS             complement(27018..28136)
                     /locus_tag="OO349_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HCR5866984.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPMAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWNCLGLYIVFLTALE
                     FKQELWGLVIVAAGFVLLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAVGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAW"
     gene            complement(28133..28600)
                     /gene="mntR"
                     /locus_tag="OO349_001895"
     CDS             complement(28133..28600)
                     /gene="mntR"
                     /locus_tag="OO349_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HCR5866985.1"
                     /translation="MSRRAGTPIAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            28786..28914
                     /gene="mntS"
                     /locus_tag="OO349_001896"
     CDS             28786..28914
                     /gene="mntS"
                     /locus_tag="OO349_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HCR5866986.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            29186..30769
                     /gene="opgE"
                     /locus_tag="OO349_001897"
     CDS             29186..30769
                     /gene="opgE"
                     /locus_tag="OO349_001897"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HCR5866987.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSGDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(30817..31332)
                     /gene="ompX"
                     /locus_tag="OO349_001898"
     CDS             complement(30817..31332)
                     /gene="ompX"
                     /locus_tag="OO349_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HCR5866988.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(31385..31450)
                     /locus_tag="OO349_001899"
     CDS             complement(31385..31450)
                     /locus_tag="OO349_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5866989.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            31685..>32564
                     /gene="rhtA"
                     /locus_tag="OO349_001900"
     CDS             31685..>32564
                     /gene="rhtA"
                     /locus_tag="OO349_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HCR5866990.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVSAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELD"
BASE COUNT         8146 a         8685 c         7879 g         7854 t
ORIGIN      
        1 gagcccgctt cgcctgcccc gagattcact tcgatcttat caccaaacca gacgcgctga
       61 gtcacaaagg tggcatcagg tttaattggc gtcacagttt caaagaagcg gaaaccccag
      121 gtcaataatt tctcagactc attaaaacgg atacggtcag ttttcgcccc cagcactacg
      181 gagattaaac gcatatcacc ctgggtagcc gaagcaacca gattatatcc cgcacctgcc
      241 gtggttcctg tcttcatgcc atcaacattc agattactgc tccacagcag acggttacgg
      301 ttaggctgac gaattttgtt gaaggtgaac tctttctctt tatgaatggc gtactcttcc
      361 ggcacatcgt ggatcaatgc tttacccagc aatgccatat cgcgcgcggt actgaactgc
      421 cccggcgcat ccaggccgtg caccgtctgg aaggtagtgt tggtcagccc cagttttttg
      481 gcataaccat tcatcagacc aataaaggac tcctggctcc cggcaacgta atctgccagc
      541 gcaatacagg cgtcattacc ggactggata atcacacctt tgttcaagtc tgccaccgaa
      601 acctgatcgc ccggtttgag gaacattacc gatgaaccac gcagtgccgg atttcccgtc
      661 gcccaggcat ctttaccgac cgtcaccata tcggtgagtt taatcttatc ggccttaagc
      721 gcctgcccaa ccacatagct ggtcatgatt ttagtcaggc tcgcaggatc cagtttctca
      781 tccgcattgc cttctgccag cactttaccg ctggcgtaat ccattaaaat ccatgcacgc
      841 gcatccacgc tcggcgcttc aacggtttgt tcggccgcga atgccgttgg ggcaaaaagg
      901 aataaaaatg cagaacccgc tgcaagacca cgaaggagag aggagtattg cgtcataaat
      961 gccacccgag tatccattca aaaaaatacg ttatatcgcc gtatgataag ttcggtgatt
     1021 aataacgcac acacaaactt aaagaaaccg taagttggta aagtttttaa agtttatgca
     1081 atatcctgcc gcttcgctgc ttctggtgtc atttttttgc cagctgttgc ttaaaagtta
     1141 actttacttc accatcggag tcctgttcag tctgtggctg cctgtcggaa aaggggtgag
     1201 tatgattacg ctgtggggtc ggaataattc aactaacgta aaaaaagtat tgctgacgct
     1261 cgaagaactg gaactacctt atgagcaaat tctcgcgggc cgtgagtttg ggataaatca
     1321 cgatgctgat tttctggcga tgaaccctaa cgggctggtg ccgttgttgc gtgacgacga
     1381 aagtgatctc attctttggg aatcaaacgc cattgtccgc tatctggcgg cacagtacgg
     1441 gcaaaaacgc ctgtggatcg actcaccggc acgtcgtgcg gaagcggaaa aatggatgga
     1501 ctgggcaaac cagacgctca gtaatgctca tcgcgggatc ctgatgggat tagtcagaac
     1561 accaccggaa gagcgcgatc aggccgccat tgatgccagt tgcaaagagt gcgacgccct
     1621 gtttgccctg ctcgatgcgg aactggcaaa agtaaaatgg ttctctggcg acgagtttgg
     1681 tgtgggcgat atcgctatcg caccgttcat ctacaatttg tttaacgttg gcctgacctg
     1741 gacaccgcgt ccaaatctgc aacgctggta tcagcaactc actgaacgcc ccgcagtgcg
     1801 caaagtggtg atgattcccg ttagctaatt gcgtgggcta actttaagta attccccact
     1861 ggactcgtcg gtgagaacgt ataaataacc gtcgggtcca gtgcgaacat cacgaattcg
     1921 ctgccctctg tccgttaaaa tacggccatc ttctgtcact ttgtcgccgt tgacgctcat
     1981 cacaatgaca tctttatctt tcagctccca ataaataatt tttgctgcca ctgggggaat
     2041 ttatcgctgt tatagaaggc catgccgctc acagcgggcg aatctttcca gtaaaaaaca
     2101 ggttgctcgg tcccggcgac gatctcccct ttcgcttccg gtatcttaaa gcctgaatag
     2161 ttgattcccc aggttgccag cggccagccg tagtttttgc ctttttgcgg gatattaatt
     2221 tcatcaccac cgcgcgggcc atgttcattc agccacagtg cattactcca cggattcatc
     2281 gccattcctt gcggattacg aatgccataa gaccagatct cggcgcgcgc accggattcc
     2341 tttataaaag gattatcatc cgggatttcg ccctggtcgg tcagacgcac cagtttgccc
     2401 tgtaatttat ccagatcctg cgccgtcggg cgctgattgt tttcgcccag agcaataaaa
     2461 agataacctt taccgtcgaa taccagccgc ccgccaaaat ggttgccggt agacagtttt
     2521 ggcatctggc ggaagacggt gcggaagtcg gtcacttttg agagatcatc acttaagcgg
     2581 ccataaccca cagcagttcc ggctttgcca tcatcgccaa cttcggaata acttaaccag
     2641 atgcggcgag actgagcaaa atcaggcgct aaaaccacgt ccagcaggcc gccctgcccg
     2701 tgcgcccaaa cgtccggaac tccggaaagc ggcgcagata atccttttcc tgcttgccag
     2761 tgacgcaact cgccgccgcg cagagtgatt aacataccgt gattatcggg taaaaaggcc
     2821 agtgcccagg gatggtcgag tttgtcttgc agtacttcga catttaccgt tgcaggagcc
     2881 gcccagagag cggaagaaag acaaataagg ggcacaagga aaaaggattg tcgatgcata
     2941 acgcctccgg attgactctg gcttaaagcg tagtcagtgg aggagataac aaattcattt
     3001 ttacaaaaac ttaaacatga agggggagac gctttctccc ccttagtttt caggccttct
     3061 caagcatggc gtgcttctgc aggctctgga tactcagcgt taagctcatc agacaatttt
     3121 caagcttatc ggcgttgacg gtaataacag tcgggcaatc atggtgccca ctcatcaaac
     3181 atactgcggc tgtcgctaat gcttcttcag catgatgaag agcactccac tcttcctgat
     3241 ccagatgaag attcaaccgc agcgatttat cgtgcagttc gcgattcagt ttaaaaaagt
     3301 tatctcgtag atgattgctt tcgctgacgg acatgtatcc ttttgccttc ctcagttgca
     3361 cgtatgcagc gaggtcaacg cgtgcgtcga tcaaccggtt cagcagatcg attcgctgtc
     3421 tgtcaacgaa catacttcga tcctcctctt tccagccttg ttgcaattaa atagtatggc
     3481 tattttttga gcaaaatgct gtccaggatc aattcttcac gccgaattaa caaaatttac
     3541 atttccttcg cctccgccag cgaatgagat tgcgatacaa attttatcca gaactctgta
     3601 gaatccctgc cctgaccttt cctgtaactg aatatttttt aagctatgag caaagtaact
     3661 ccccagccga aaatcggctt tgtttccctt ggctgtccga aaaaccttgt cgattcagag
     3721 cgtattctca ccgaactccg cactgaaggt tatgacgtgg taccgagcta tgacgatgcg
     3781 aacatggtga tcgtcaacac ctgcggcttt attgacagcg cggtacaaga atcactggaa
     3841 gccattggtg aagcgttgaa tgaaaacggc aaggtaattg tgaccggttg tctgggggca
     3901 aaagaagatc agatccgcga agtccacccg aaagtgctgg aaatcaccgg gcctcatagc
     3961 tatgagcagg ttctggagca cgttcatcac tacgtgccaa aaccgaaaca caacccattc
     4021 ctgagcctgg tgccagaaca aggtgtgaaa ctgacgccgc gtcattatgc ctatctgaaa
     4081 atttcggaag gctgtaatca ccgctgcacc ttctgcatta ttccgtctat gcgcggcgac
     4141 ctggtgagcc gtccgattgg cgaagtgtta agtgaagcga aacgtctggt agatgcgggc
     4201 gttaaagaga ttctggtgat ctcgcaggat acttccgcct atggcgttga tgttaaacat
     4261 cgtactggct tccacaacgg cgagccggta aaaaccagca tggtcagcct gtgcgaacag
     4321 ttatcgaaac tggggatctg gacacgtctg cactacgttt acccttatcc gcatgtggac
     4381 gacgtcatcc cactgatggc agaaggcaaa atcctgccgt atctggacat tccgttgcag
     4441 cacgccagcc cgcgcattct caaactgatg aagcgtccgg gttctgtaga tcgccaactg
     4501 gcgcgcatca aacagtggcg cgaaatctgc ccggaactga ccctacgctc aacctttatt
     4561 gtcggcttcc ctggcgagac ggaagaagat ttccagatgc tactcgactt cctgaaagaa
     4621 gcacgtctgg atcgcgttgg ctgctttaaa tacagcccgg ttgaaggtgc agacgccaat
     4681 gccctgcctg accaggttcc ggaagaagtg aaagaagaac gctggaaccg tttcatgcag
     4741 ctgcagcagc agatttccgc cgagcgcctg caagagaaag tgggccgtga aattctggtg
     4801 attatcgacg aagtggacga agaaggcgcg attggtcgca gcatggcaga tgcaccggaa
     4861 atcgacggcg cggtttatct caatggtgaa accaacgtta agccgggtga tatcctgcgt
     4921 gtgaaagtcg agcacgccga tgagtacgat ttgtggggta gccgggttta attaattcag
     4981 gccgggcaaa atcatttgtc cggcctgcga ataatgatta ctgccagcta atctgtgccc
     5041 tgccgttttt cttactctta tacagccgct catccgccag cattttcaag cgttcgacgg
     5101 tagtacattc atttgaatgc gctacaccga tactggtgct taaattaatg cttcccacct
     5161 ggaaatgaac gatttctttt agtatcgata gcaactcttc cagcgtgccg ccagtataaa
     5221 tcaccgcaaa ttcgtcacct ccgaagcggt agactttacc tttatctacc gggatctgtg
     5281 atttaaggat tttcaccact ttcatcaaca cttcatcgcc cagcaaatga ccgaaggtgt
     5341 cgttaatgtt tttgaattta tcgacgtcaa acagacacaa ataaccgcct ttttccggca
     5401 gcgcttttaa atcttcatca aaggattttc tgcccattgc tttggtcagg tcgtccagga
     5461 acgccgagtt atacatactg tagagttttt tgacatcggc agccgaaaag aaaagcccgc
     5521 catccagtac gcgttcaaaa tggttattaa tgatggcttc tttctccaca ataccacgat
     5581 taatcactct gcgtaccaga tagaggcaac tggcacccaa tatcactgcc accaatgcgg
     5641 caaagaaaat aatcaatgta caaacccgca aataaacatc ttttttatac gcaaatacgt
     5701 acaatccggt cggcaaatag gcgaagtgct ccatcgcttc cgaaacggtg aagatggcgt
     5761 ttttagcatt atattcatca ccggtcaccg gtttaccgtt cttaaataga gagaacatca
     5821 ggttctcgct gtccatcacc agcgattttg acagtaaaga aaaacgctcc agaccaaaca
     5881 aaaatatcag actacgattg tgagttaccg gatagaggta atagacgaag gttttaccgt
     5941 caaaagagat aaaccctgta tttttgacat gtcgatactt cacaacctgc ataatatctg
     6001 cacaataatt tttctcgaac tcctttacct gaatacattc gtcactggaa atcagataag
     6061 gaccgacatc gtacaaataa gagggggaaa ccgagtcggc gtaagctgtg cgcaaaccaa
     6121 ccaacagctg ttcctccgtg ggatttagcg tgttgataag atcaattgcc acgctacgcc
     6181 gatattcagc aatatttgcc accgcaaaag cagtatcaat aacggactgt ttcaggctat
     6241 acacccgttc ctttaccatt tgagtgtaga tcccgcaggc aactgctgaa atcatgataa
     6301 aaatcagcag actgactgta agtacgaact tattgattct ggacatacgt aaactactct
     6361 tttactaatt ttccactttt atcccaagcg gagaatggca tgggtttctg gtacagatac
     6421 ccctgagcct gaacacatcc tgatttaatt aattcgcgcg caacgtcttc gctctctacg
     6481 ccttccgcaa tcaccgggat ccggttatag cgtgccagat cgaaaataat gcgcacaatt
     6541 tttcccgcag taccgaaaat atcgccggta aattgcgagt ccacctttat gctgtctgga
     6601 ttaatttcat aaaaacgttt caggtttgac aaaccggtgc caaagtcatc aatcgatatc
     6661 cgtacccctt gtttacgcag ttgttcaata ttgagataaa aatgttcatt aattcgctcg
     6721 atcacggttt cagtgatctc aacacagagg ctgttcgcct tcaggccatt ttctttcatg
     6781 atggtggtaa aacgctcatg aaagtgcggt tcgtttaact gccagggcga aacattaata
     6841 tgcagtaaga aatcatcctt cagcgacgca cgttgactaa aatgtctgaa ttcacccatt
     6901 gccgttttaa taatctgata acccagctca ttgataaacc cgatatcttc agcaatggta
     6961 atgaacttca gcggtgaaat gatcccccgc tccgcagaaa cccaacggca caacgcctcc
     7021 gcgctcagaa tggcgcgggt gttaatatca acgatcggtt gatagacgag gaatagttcg
     7081 ccaatttcca ttgcatggcg aatattacgc gcaatccaga tattctcttc tatttcgttg
     7141 agtttcgcgt catcacagaa aataaactcg gagaacatat ggtcacggat actggaaatc
     7201 gccagtttca gtttttctgc gtaactccag gcgttttccc cctgcaaagg ttcacaaatg
     7261 gcagacttat ggatgcgcag attgcggtag ccaaagctgc tgtcaaaatc gtttaccatc
     7321 gctattttac gggtaaatgt ctgtacattt tcccgacaca gaagtaaata gagatcggca
     7381 ttgaatgtca cgatatcgca gcattgcgca tatttctcgg caattcgctg acacaatttc
     7441 ctgacgattt tattgcgttc atcaacacca aacaccgcct ccacggcttc cagaccacac
     7501 agtttgatga tccccacagc cagatactga ttattgttgg actcaataat cgccatattt
     7561 ttaataaaac cgctttcctg atcgtaagag atcagtgaga gcaatttatt tgaccgttca
     7621 atttcggcat ctttcatttc cgcgatcgaa tttttgatgc tgataatctc tttcgcatcg
     7681 ccttcgcgaa ttttcagagc ctcgatctga tccgttgagt cgggcaaaaa cgccagcgta
     7741 ttaaccagct cggtcatttc agaaaattgc cgacggaaca cacgagtggt catgaaatat
     7801 accaccagca acacgataat aatgaacgca aacggtagat aaaaaagttt atctgatgcg
     7861 ttcaataaat taccgccagg ataataaaaa tagatttcga acaatgcatt atttggcact
     7921 gctgatgtct ttgcaatata ctccgtatcg acagaatcaa taatattata attaccttcg
     7981 ctattataaa taatcagttt ttcacgagcg agttgattct tggcaatgat ctcatgacct
     8041 tgtttcaaaa atagaacttc atcattagcg ctgatttgat caagatactc gttcagatac
     8101 cgccagttta aatcgacatg aattttatct accgctaaca aatcgtgatc tttgccgttg
     8161 atacgtagcg taaaagagat acatttttta cccggacgat caaagcaatt gtatgccgga
     8221 ttccagctgt tgttagagtc atctgaagcc accgaccacg gtcgtttcga tgggttaaaa
     8281 tcggcaaaaa taacccgccc tgactcgtcg acaagcggtc catttacttg ttcctgatga
     8341 taaacaacga ttttatcatt cgccctccgc gaaaagagat aatatttatt atcatcaagt
     8401 accagtccca ccgagtctaa aaatggcatc atttccattt tcagaatcac ggcgcgttga
     8461 atctcctctg tattttttaa taaatacagt gagtccttta atgtcagggc cgaacgttca
     8521 atatctttaa agaaatagcc aacgtttttc tgaataagtt caaagcggct atcggcacgt
     8581 tttaccgcgc gttccgatac cagttgatag ttaatcatga aaaaaagacc aataaatgac
     8641 agtgctgcca ataaaaataa aattatcagc cttatcttta tcttttcgta taaactcagc
     8701 atgttaatac atttaacaaa ataactatct gaacgctgtg ccgctgcaaa aatagtccct
     8761 tgctgcggct ttttaaccaa attacccgat tctacaaaat cagcagaaag ttacaacact
     8821 tgcttagggc aataatttcc tgtgaggtga ttaccctttc aagcaatatt caaacgtaac
     8881 tatcctttaa ttttcggatc cagcgcatcg cgtaaaccat cgcccaacaa attgaacgcc
     8941 agtacggtca gaaaaatagc cagggccgga aaaacagcga catgcggcgc gataaccata
     9001 tccgctcgtg cctcattgag cattgctccc cactctggtg tcggcggctg cgcaccgagg
     9061 ccgagaaatg acaggctggc ggcagagata atcgaggtgc caatgcgcat ggtgaaaaac
     9121 accacgatag aagagacggt cccaggcagg atatgacgca ataaaatggt catatcgctg
     9181 gcaccaatac tgcgtgctga ctcaataaag gtttgctgtt tcaacaccag cgtgttgccg
     9241 cgcaccaggc gggcaaacgc ggggatggaa aaaatggcga ctgcgataat cacgttagca
     9301 atgccgcttc ccaacaccgc aacaacagcg atcgccagta aaatacccgg gaaggcaaac
     9361 agcacatcgc aaatgcgcat gatcagccga tcccaccagc cttcataata tccagccaac
     9421 aagcccagca acgtccctat cgccacaccg ataaacacgg caaacacgcc cgccgccagc
     9481 gagatttgcg caccaaccag gacacggctg aaaatgtcgc gccccagtga atcgacgcca
     9541 aaccagtgct gcaaagaagg tccgttattc agattgtcat aatcaaaata attttcggcg
     9601 tcatagggag cgatccagcg tgcaaaaatg gccaccacaa tcaataaaat aacgaataat
     9661 gcggcggtca tcgccatatg ctgacggcga aatcgtcgcc agaattcatg ccacggtgta
     9721 cgtacctggt caggtttgac cagtggcatg gcgtttaaca ccgcctgacg tcgccagtta
     9781 aatagtcgca tccttacttg tacctgatag ccgggttaat ggcggcgtaa agcacatcca
     9841 ccactaagtt gataagaata aattccagcg agaaaagcag aatttccgcc tgaatcaccg
     9901 ggtaatcgcg catttctaca gagtcaacga gtaagcgtcc aagtccgggc cagttgaaaa
     9961 ctttctccac cacgatagaa ccaccgagca aaaagccaaa ctgtaagccc atcatggtca
    10021 ctaccgggat catcgcgttg cgtagcccgt gtttgaggac aacccaggtt tcgctcaccc
    10081 ctttcgccct cgcggtacgc atataatctt cgcttaaaac gtcgacaaat gacgcacggg
    10141 taaagcgcgc catcacggcg gccactgccg cgccgagcgt cagggagggt aaaatgtagt
    10201 gctgccaact gtctgctccc acagtaggca gccagcccag ttcaacggag aatacctgaa
    10261 ttaaaaacat ccccagcgca aatgccggaa acgagatccc cgacaccgca atggtcatac
    10321 tcaatcgatc cggccaacga ttacgccaga cggcggcgat aattcccgcc gccataccaa
    10381 atatcaccgc ccagaccata ctggttatgg tcagccacag cgttggcata aagcggctgg
    10441 caatctcatc ggcaaccgga cgacgcgaca ccatcgacag gccaaaatcc ccctgcacag
    10501 cattgctgat atagtgccag aactggtgat acagcggctg atccaacccc agctgctgac
    10561 gcaccagttc tataacctgc gcatcagctt cgggcccggc aatcaatcgc gccggatcgc
    10621 cgggcagcat gtggacaaat aaaaacacca gcaccgagac gataaacagc gtcggaatca
    10681 accccagtaa tcgtttgata acgtaattaa gcattccact ccctgcgttg cttattgcaa
    10741 atccgcgtct tcaaagctga agccggtatc tggcatgatc caaaaaccgg tcaggttttt
    10801 actgtgtgcc gacaccagtt tttctaccac cagcgggatc cacggcgatt cttgccagat
    10861 gatatcctgc gccgccttat ataagcgggt cttttccgcc ggatcattcg ttttcagtgc
    10921 ctgagccagg aagtcatcca cctgtttatt gctgtaaaac gcggtattaa acagcgtcgg
    10981 tggccagttt tgcgaggcaa acagcggcga tagtgcccag tcggcttcgc cggttgaagc
    11041 cgaccagcca gtgtagaaca tccgcacgcc actctctttt tgccctttac cttcaacttc
    11101 tgccgcccgc tgtccggcat ccatcgcggt cacctgggct ttaatcccga cctgcgctaa
    11161 ctgctgctgg gtaaattgca gcactttctg cgcggtgctg tggttatgtg acgaccacag
    11221 cgtggtactg aaaccgttgg gatatcccgc ctctttcagt aattcgcgcg ctttcactgg
    11281 atcgtaaggc cacggtttat aactttgcgc gtaggcgata cttggcggta ccacaccagt
    11341 agctggcgtt gcatagcccg caaaggcaac tttcaccagc gccggacggt taatggcgta
    11401 attcagcgcc tcacggacct tcgggttatc gaacggcttt tgcgtcacgt tcatactgat
    11461 ataacgctgc ataattgacg gactggccat caactcgata tttttgtttt tctccagcag
    11521 tgtggcctgc tcgtagggaa tggggaaagc aaactgcgct tcaccggttt gcagcattgc
    11581 cgcgcgggtg ttgttatccg ccaccggacg ccaggttatg ctgtccagtt tgggcaatcc
    11641 tggctgccag taacccgcga attttttcac cttcacaaaa tcggtctgat tccaggtatc
    11701 cagttcatac ggtccggttc ccaccggatg aaaaccaatc tccttgccat atttttccag
    11761 cgctgtcggt gaaatcatcg cggtcgccgg atgggcaaga atattaataa acgctgagaa
    11821 cggctgtttg agggtaatct ttaccgttgt cggatcgatc gcctccgttt tggcaatatt
    11881 cttatacagg ttatagcgtt taagatgatt cgccggatcg ctggcccggt ccagattcgc
    11941 ttttaccgcc actgcgttga aatcggtgcc atcctggaat ttaattcctt cccgcaattt
    12001 cacggtgtaa gtaatgccgt catcggaaac ggtataactc tccgccagca cgtttttcag
    12061 tttcatctct ttatccagac cgaacagccc ctggtaaaac gatttcgcta cggcctgaga
    12121 taacgtgtca ttcgcgtcat acggatcgag cgtggtgaaa ttcgatccta ccgccaccac
    12181 cacatctttg gcagcgaatg cacaagatgc catcaacgct gtcgcaatgc ccagcgccac
    12241 taacccacta cggtgtacag ctcttgccat tttattctcc gcctgaatgt tatctacgca
    12301 tgaatgcgta ttctgattgt ggttgtgcga cgtaatgccc cggcccgacg cattgcaacg
    12361 agacggctgc cacctcttcg ccacgcagat gaatattgct gggaagatcg tccgacagca
    12421 gtacacgctg cggtcgttgt cgggacggtt cagcgaccgg aactgccgcc agtaatttac
    12481 gcgtataagg atgctgcggg ttttcgaaga ccgcgcaccg tgaaccaatt tcaacaattt
    12541 gcccgagata catcaccgcc acacgatgac taatccgctc taccacagcc atatcgtggg
    12601 agataaacag atacgcaatg ccgaaatcac gctggagatc gagcaacaag ttgataatct
    12661 gcccgcgaat agaaacatcc agcgccgaaa cggcttcgtc ggcaatgatc acttttggat
    12721 tcaatgccaa cgcgcgagca atgcagatgc gctggcgctg accgccggaa aactcatgcg
    12781 ggtaacgcca ggcatgttca ggtaacaggc ccacgcgctc cagcaaccac gcaacgcgtg
    12841 cagccgcgtc tttacctggc aataaaccgt gtacacgcag cggttcgata atcgaatcac
    12901 cgatggtctg acgtgggtcc agcgaagcgt aagggtcctg aaaaataaac tgaatatccc
    12961 ggcgcaatgc ctgaagtttg ccaggtgaca aggtatcgat tcgctgaccg ttaaagataa
    13021 tttcgccgcc ctgcgattcg accaggcgca gcaacgcccg cccggtagtg gatttaccgc
    13081 tgccagactc acccaccagc gatagcgttt cgccaggcca gagatcaaaa ctgactttct
    13141 caacggcatg cacttcccgc gttacgcgat tcaacaaacc gctgcgcaaa gggaaacggg
    13201 tgacaagatt acgcacccgt aaaacaggtt cgccatccac caccgttttc tgctcgatgg
    13261 ggggttcctg tttcgctgga tgttcaagcg atatcaatgg gaaacgtcgg ggataatcta
    13321 accctttcat cgcaccaagt tgcggaacag cagctaacag cgcacgggtg taaggatgtt
    13381 gcggtgcatg aaaaatctgt tcgacggtac ccgtttccac cgcctcgccc tgatacatca
    13441 ccagtacccg atcggcaatc tctgccacca cgcccatatc gtgagtgata aagataacgc
    13501 ccatcgacat ctctttttgc aatactttga ttaattgcag gatctgcgcc tgaatagtga
    13561 catccagcgc ggtggttggc tcatcggcaa tcagcaccgc cgggcggcat gacagcgcca
    13621 tcgcaatcat cactcgctgg cgcatcccgc cagagagttg atgcgaataa cgtgaaagaa
    13681 tggtttgtgc ctccggaatg cgtacctgat ccagcatccg cttcgcctcg accatcgctt
    13741 cttcacgact ggcgttctga tgcagacgaa ttgattcggc aatctgttca cccacagtaa
    13801 atactgggtt cagcgatgtc atcggctcct gaaatatcat cgccatatcc gcaccgcgca
    13861 catggcgcat ttgtgcagcg ctctgctcgc taagttcaat cacatcacga ctgcgccgcc
    13921 gcaacagcat tttatcgcac tgtactaaac cgcccgcctg ttccaacaga cgcatcaatg
    13981 ccagcgcagt cactgactta ccggagccgg attcgcctac aattgccagc gtctcaccgc
    14041 gttgcagact aaaagagaga ttgcggaccg cagctatttt ctgctggtcc tgcataaagg
    14101 caatattaag attttcaacc gccagcacat taccggcatc aagttcatca ctgtgtggca
    14161 acggtgtccc ctttttcacg gtagataccg gtggttggcg tatcgcctgc gtagccccag
    14221 gcgcgataca ttccttcggt gttaaacggt agcgcgacat tcccttcatg gtcgatagcg
    14281 attaagccac cgctaccgcc aagcgcaggg agtttttcca ttactacccg ctcgcaggct
    14341 tccgcgagac ttaatccgcc gtaatccatt aacgcggcga tgtcatatgc cgccagcgcg
    14401 cggatgaaga cttcgcctgt gccggtacaa gaaaccgcca cactggcgtt attggcgtag
    14461 catccggcac ccactaaggg actatcgcca actcgtccgg gtaatttatt ggtcattccg
    14521 cctgtggacg tggctgccgc cagattgccg tctaaatcca acgccacggc ccccacggtg
    14581 cccattttct gtttttcatc cagtggcgca ccgctatggt cgaggactgt tgccccttcc
    14641 tcgcgcgctg ccagtagttg ttcataacgc aaaggcgtgg agaaaatctc cggtgagacg
    14701 cgctccatgc catgagcaaa cgcaaaattt tctgcccctt cgccaatcat catcacatgc
    14761 gggctttgct ccatcaccag ccgggcggca agaaccggat tacgcagatg actaacgccc
    14821 gccaccgcac cggctttcag ggtgttacca tccatcacac aggcgtccag ttcatgggtt
    14881 tcatcacgcg taaagacagc gccaattccg gcgttaaaca gtggacactc ttccagcaga
    14941 cgcaccgctt ccgtcaccac atccagcgca ctttcgccgg cttccagcat tttctgcccg
    15001 gtttcaacaa tggcagacaa cgcctcgatg tagcgtaatt cctgttgcag actcatctgc
    15061 gcgcggctaa ttgcacctgc gccaccatga attgcaatga ctgctttgcc catagtattc
    15121 gtccattaag ggtggctcgc cacttttgct ataaatatca gaatttttat gaattactca
    15181 ttcaattttt gaatatagaa agatggatat ggcatgtaaa ggcaggagcc aacgctacgc
    15241 ccgaggtggc atacagtcta tggcgttttt ctgacataat aggcaaattc gattttgcct
    15301 ccgcaggagt gttttcatgg aatttaccac cggattgatg tcgctcgaca ccgcgcttaa
    15361 tgagatgctt tctcgcgtta ccccactgac cacccaggaa acgctgccac tggttcagtg
    15421 ttttggtcgt attctggcga gcgatgtcgt ttcgccactg gatgtcccgg ggtttgataa
    15481 ctccgctatg gacggctacg cggtgcgttt agccgatatt gcctccgggc aaccgctgcc
    15541 cgttgccggt aaatcctttg ccggtcagcc ataccatggt gaatggcctg cgggtacctg
    15601 cattcgtatt atgaccggtg cgccggtgcc ggaaggctgc gaagcggtgg tgatgcagga
    15661 gcagactgaa caaacggaca atggcgtgcg ttttactgct gaagtgcgta gcgggcaaaa
    15721 tattcgccgt cgcggtgaag atatctctgc aggtgcggtt gttttcccgg cgggaactcg
    15781 cctgactacc gcagagctgc cagtaattgc ttcactgggg attgccgaag ttccggtgat
    15841 tcgtaaagtg cgtgtagcgc ttttttctac cggtgatgaa ctccagttgc ccggtcagcc
    15901 gctgggcgac ggccaaatct acgacaccaa ccgtctcgcc gtacacctga tgttagaaca
    15961 gctgggttgc gaggtaatca acttagggat tatccgcgac gatccacatg ccctgcgcgc
    16021 cgcatttatt gaagccgaca gccaggcgga tgtagtgatc agttccggcg gtgtttcagt
    16081 gggtgaagcg gattacacca aaatgattct tgaagagctg ggggagatcg ccttctggaa
    16141 gctggcgatt aaaccaggta aaccgttcgc gttcggtaaa ctcagcaata gctggttctg
    16201 cggcctgccg ggcaacccgg tttcagcgac gctgaccttc tatcaactgg tacagccttt
    16261 gctggcaaaa ctaagcggca acaccgccag cggcctgccc gcgcgccagc gcgtacgcac
    16321 agcgtcccgc ctgaaaaaaa cgccaggacg tcttgatttc cagcgcggcg tgctgcaacg
    16381 caacgccgac ggtgaacttg aagtgacgac caccggacat cagggttcac atatatttag
    16441 ctcctttagt ctcggcaact gctttatcgt gctggaacgc gatcgcggca atgtggacgt
    16501 gggcgaatgg gtggaagtag aaccgtttaa cgcgttgttc ggaggcctgt aatggcggaa
    16561 ctcagcgatc aggagatgct gcgctacaac cggcaaatca ttctgcgcgg ttttgatttt
    16621 gacggtcagg aggcgctaaa agattctcgt gtgctggtag tcggcctcgg tggactcggc
    16681 tgcgcagctt cgcagtatct ggcaagcgcc ggtgtcggca acctgacgct gctcgacttc
    16741 gacacggttt cgctctcgaa tctgcaacgc cagacactgc acagtgatgc cacggtcggg
    16801 caaccgaagg tggaatccgc ccgtgacgcc ctgacgcgga tcaacccaca tatcgcgatt
    16861 acgccagtca atgcactgct agatgacgca gaacttgcag cattgattgc tgaacacgat
    16921 ctggtgctcg actgtacgga taacgttgcg gtacgtaatc aactgaacgc aggctgtttt
    16981 gccgcgaagg tgccgctggt ttccggcgcg gcaattcgta tggaagggca aatcacggtc
    17041 ttcacttatc aggacggcga accgtgctat cgctgcctta gccgtttgtt tggcgagaac
    17101 gcgttaacct gcgtagaagc aggcgtgatg gcaccgttga taggcgtaat tggttcgttg
    17161 caggcaatgg aagcgatcaa actgctggca ggttatggca aaccggccag cgggaaaatc
    17221 gtcatgtacg atgcgatgac ctgtcagttt cgcgaaatga aactgatgcg caatccgggg
    17281 tgtgaggtgt gcgggcagta attgccacct ccaagcctga taagacgcga caagcgtcgc
    17341 atcaggcatt tgctccagac gccagatgcg gcgtgaacgc cttatctggc ctacggttcg
    17401 ggcacagatt tccaagcgcg gcaggcatcg gtgcactccg ccacgtcatc agcaatttat
    17461 gaggatgcag aacgtccggt taaatcgacg ttctgccaaa cgctccctgc cagtcctgct
    17521 caaacttcgt cacagcggct tccaccgccg gatagctaat catctgttgt gccacatcca
    17581 gtggcagagt aattgattca catccggcca gtaagcagtc cagcgcctga cgcggggttt
    17641 tgaaactcgc tgccagcact ttcgcctgcg gcgcatgcat tttcaataac tggtgtaagt
    17701 cggtcacagt ctgaatgccg ctaccgccct gagcatcaat acgattaacg taaggcgcaa
    17761 catattccgc acctgccagc gccgacagca gcccttgtgc cgcgccatat accgcggttc
    17821 ccagcgtcgg aatcccttcc gcttttaaca tcttaatcgc tgccagcccc tcggctgtca
    17881 ccggaacttt caccacgata tccgcaataa tagaacgcag cttaagcgcg tcattcacca
    17941 tcccttcagc agtggtagcc attacctggg caaacagacg cccctgaccg cccatcgctt
    18001 catgaagttg cggaagcaca acatccagcg gttttttacc cgcggcgata atgcttgggt
    18061 tagtggtcac acccgccagc ggaaaaatac gtgacagcgc cttcaccgca acaacgtctg
    18121 aagtatccag atacagttcc ataaccatcc tcaaaatgta ctttttgtct gaatgtagac
    18181 tatcacggga aaactctctg aacagttgac gcacatcaat ctgactttca ttcgaaagta
    18241 ttttaatgtt tgaacgaaac agagaggcaa ctatgatttt caatattcag cgctactcga
    18301 cccatgatgg ccccggtatc cgcacggtcg tatttcttaa aggctgttcg ctgggctgcc
    18361 gctggtgtca gaacccggaa agccgcgccc gtacgcagga tctgctgtat gacgcacgac
    18421 tgtgtctgga aggctgcgag ctgtgcgcta aggccgcgcc ggaagtgatt gagcgcgcgc
    18481 tgaatggttt gcttattcat cgggaaaagt taaccccgga gcatctgacg gcgttaaccg
    18541 actgctgtcc gacacaggcg ttaaccgtgt gtggtgaagt gaaaagcgtt gaggagatca
    18601 tggcgaccgt tctgcgcgat aaaccgtttt acgatcgcag cggcggcggc ttaacgcttt
    18661 cgggtggtga acccttcatg cagccggaaa tggcgacggc gctgctgcaa gcctgccacg
    18721 aggcaggcat tcatactgcg gtagaaacct gtctgcatgt gccgtggaaa tatatcgccc
    18781 cttctctgcc ctatatcgat ctgtttcttg ccgatttaaa acacgttgcc gacgcgccgt
    18841 ttaaacagtg gaccgacggt aacgccgcca gggtgctgga taacctgaaa aaactcgccg
    18901 cagcgggcaa aaaaatcatt atccgcgtgc cgctgattca gggctttaat gccgacgaaa
    18961 cctctgtaaa agccattacc gattttgccg ccgacgagct gcacgttggc gaaattcatt
    19021 ttctgcccta ccacacgctg ggcatcaaca aatatcactt acttaatctg ccctatgacg
    19081 ccccggaaaa accgcttgat gcgccagagc tgctcgattt tgcccagcag tatgcctgcc
    19141 agaaagggtt aaccgcgacc ttacgaggat aacaaccatg accacactga aactggacac
    19201 gctcagcgac cgcattaaag cgcacaaaaa tgcgctggtg catattgtga agccgccagt
    19261 ctgtaccgag cgcgcgcagc actataccga gatgtatcaa caacatctcg ataagccgat
    19321 cccggtacgt cgcgcgctgg cactggcgca tcacctggcg aatcgcacca tctggatcaa
    19381 acacgatgag ttgatcattg gcaaccaggc aagcgaagtt cgcgccgcgc cgatcttccc
    19441 ggaatatact gtctcgtgga tcgaaaaaga gattgatgat ctggcagatc gtcccggtgc
    19501 tggctttgcg gtgagcgaag agaacaaacg tgttctgcat gaagtgtgcc catggtggcg
    19561 cggtcagacg gtacaggatc gctgctatgg catgtttacc gatgagcaaa aaggtctgct
    19621 ggcgaccggc atcattaaag cggaaggcaa tatgacctcc ggcgatgcgc acctggcggt
    19681 caatttcccg ctgctgctgg aaaaagggct tgatggtctg cgtgagaaag tagcggaacg
    19741 tcgctcgcgc atcaatctga cggtgctgga agatttgcac ggcgaacagt tcctgaaagc
    19801 gattgatatc gtgctggtgg cagtcagtga acacattgaa cgtttcgctg ccctggcgcg
    19861 tgcaatggcc gcgaccgaaa cccgcgaaag ccgtcgcgat gaactgctgg cgatggcaga
    19921 aaactgcgat cttatcgccc accagccgcc gcagactttc tggcaggcgc tgcaactgtg
    19981 ttacttcatc cagttgattt tgcagattga atctaacggt cactcggtgt cgtttggtcg
    20041 tatggatcag tatctctacc cgtactatcg ccgcgacgtt gaactgaacc agacgctgga
    20101 tcgcgaacac gccatcgaat tgctgcatag ctgctggttg aaattgctgg aagtgaacaa
    20161 aatccgctcc ggctcccact caaaagcctc tgcgggaagt ccgctgtatc agaacgtcac
    20221 cattggcgga caaaatctgg ttgatggtca accaatggac gcggtgaatc cactctctta
    20281 cgcgatcctc gaatcctgcg gtcgcctgcg ttccactcag cctaacctca gcgtgcgtta
    20341 ccatgcagga atgagtaacg atttcctcga cgcctgcgta catgtgatcc gctgcggctt
    20401 cgggatgccg gcattcaaca acgacgaaat cgtgatcccg gaatttatta aactcggtat
    20461 tgaaccgcag gacgcttatg actacgcagc gattggttgt atagaaaccg ccgtcggtgg
    20521 caaatggggc tatcgctgta ccggcatgag ctttatcaac ttcgcccgcg tgatgctggc
    20581 ggcgctggaa ggcgggcgtg atgccaccag cggcaaagtg ttcctgccac aagaaaaagc
    20641 gttgtcggca ggtaacttca acaacttcga tgaagtgatg gacgcgtggg atacgcaaat
    20701 ccgttactac acgcgtaaat caatcgaaat cgaatatgtc gtcgacacca tgctggaaga
    20761 gaacgtgcac gatattctct gctcggcgct ggtggatgac tgtattgagc gagcgaaaag
    20821 tatcaagcaa ggcggcgcga aatatgactg ggtttctggc ctgcaggtcg gtattgccaa
    20881 cctcggcaac agcctggcgg cagtgaagaa actggtgttt gaacagggcg cgattggtca
    20941 gcaacagctg gctgccgcac tggcggatga cttcgatggc ctgactcatg agcaactgcg
    21001 tcagcggctg attaacggtg cgccgaagta cggcaacgac gatgataccg tcgatacgct
    21061 gctggctcgc gcttatcaga cctatatcga cgaactgaaa cagtaccata acccacgcta
    21121 cggtcgtggt ccggttggcg gcaactatta cgcgggtacg tcgtctatct ccgctaacgt
    21181 accgtttggc gcgcaaacta tggcaacgcc ggacgggcgt aaagcccaca ccccgctggc
    21241 agaaggcgct agtccggcct ctggtactga ccatctcggc cctactgcgg tcattggctc
    21301 agtgggtaaa ctgcctacgg cagcgattct cggcggcgtg ttgctcaacc agaaactgaa
    21361 tccggcaacg ctggagaacg aatctgacaa gcagaaactg atgatcctgc tgcgtacctt
    21421 ctttgaagtg cataaaggct ggcatattca gtacaacatc gtttcccgcg aaacgctgct
    21481 ggaggcgaaa aaacatccgg atcagtaccg cgatctggta gtgcgtgtcg cgggttattc
    21541 cgccttcttc accgcgctct ctccagacgc tcaggacgat atcatcgccc gtactgaaca
    21601 tatgctgtaa tctcttcggc ccggctgcgg cgtcgccggg ctaaatcatt cacatcatca
    21661 atttcatcct tactttcatt cgaaatataa tttgtgctct gcgtcacatt gttattacac
    21721 tgttacggtt tcattttcga gccaggagca cacctatgag cgtaaaagtt atcgtcacag
    21781 atatggacgg tacttttctt aacgacgcca aaacgtacaa ccaaccacgt tttatggcgc
    21841 aatatcagga actgaaaaag cgcggcatta agttcgttgt cgccagcggt aatcagtatt
    21901 accagcttat ttcgttcttt cctgagctaa aggatgagat ctcttttgtc gcggaaaacg
    21961 gcgcactggt ttacgaacat ggcaagcaac tgttccacgg cgaactgacc cgacatgaat
    22021 cgcggattgt tattggcgag ttgctaaaag ataagcaact caattttgtc gcctgcggtc
    22081 tgcaaagtgc atatgtcagc gaaaatgccc ccgaagcatt tgtcgcactg atggcaaaac
    22141 actaccatcg cctgaaacct gtaaaagatt atcaggagat tgacgacgta ctgttcaagt
    22201 tttcgctcaa cctgccggat gaacaaatcc cgttagtgat cgacaaacta cacatagcgc
    22261 tggatggcat tatgaaaccc gtcaccagtg gttttggctt tatcgacctg attattcccg
    22321 gtctacataa agcaaacggt atttcgcggt tactgaaacg ctgggatctg tcaccgcaaa
    22381 atgtggtagc gattggcgac agcggtaacg atgcggagat gctgaaaatg gcgcgttatt
    22441 cctttgcgat gggcaatgct gcggaaaaca ttaaacaaat cgcccgttac gctaccgatg
    22501 ataataatca tgaaggcgcg ctgaatgtga ttcaggctgt gctggataac acatcccctt
    22561 ttaacagctg acctctctac cggagccatg cgctccggtt ttcccttctt tttacatcct
    22621 gtacatcaca tcaaatcatt aaacttgcat taacttattt ataacttgta ttgtcacatg
    22681 caaattagca tagtttcgaa tgaaagttaa tgcgaggtgg ttatggcttc tacttttacc
    22741 agcgacacat tgcctgccga tcacaaagca gctatccgtc agatgaagca cgcgctgcgg
    22801 gcgcagcttg gcgatgtcca gcagatcttt aatcagctaa gcgatgacat tgccacgcga
    22861 gtggctgaaa tcaacgcact caaagcacag ggcgatgccg tctggccggt gctgtcttat
    22921 gccgatatca aagcaggtca tgttactgca gagcagcgcg aacagattaa acgtcgcggt
    22981 tgtgcggtga taaaaggcca tttcccccgc gaacaagcgc taggctggga tcagtcgatg
    23041 ctggactatc tgaaccgcaa ccgctttgac gaggtctaca aaggccccgg cgataatttc
    23101 ttcgggacgc tcagcgcttc acgtcccgag atttacccca tctactggtc gcaggcgcaa
    23161 atgcaggccc gccagagtga agaaatggcg aatgcgcagt cgtttctcaa tcgtctgtgg
    23221 acatttgaaa gtgatggaaa gcaatggttt aacccggatg tgagcgtcat ctaccctgac
    23281 cgtatccgcc gccttccgcc cggaacgacc tccaaaggtc ttggagcgca taccgactcc
    23341 ggggcgctgg aacgctggct gcttccagcg tatcagcacg ttttcgccaa cgtctttaat
    23401 ggcaatctgg cgaaatacga tccctggcat gcggcacatc gtacggaagt tgaagagtac
    23461 acggtggaca acaccaccaa atgttccgtg tttcggacat tccagggctg gactgcgctc
    23521 tcggatatgc tgcctggtca ggggttgctg cacgtcgtgc ccattcctga agccatggcg
    23581 tacgtactgt tacgtccgct gcttgatgat gtgccggagg atgaactgtg cggcgtagcg
    23641 cccggaagag tgttgccggt atcagagcaa tggcatccac tgttaattga ggcgttaacc
    23701 agcattccaa aactcgaggc cggagactcc gtctggtggc actgcgacgt catccattcc
    23761 gttgcccccg ttgaaaatca acagggttgg ggcaacgtga tgtacattcc tgcggcaccg
    23821 atgtgcgaga aaaatcttgc ctacgcgcac aaggtgaagg ccgcactgga aaaaggcgca
    23881 tcgccgggcg acttcccgcg cgaggactat gaaacaaact gggaaggacg ctttacgctt
    23941 gccgacctca acattcacgg taagcgagcg ttgggcatgg atgtttgaga gttaatggaa
    24001 gtgaattgat gtgggattaa gaaaatcgat tcgacgttac tcagattgct gacaagtgcg
    24061 ctttcttaat gcctgatgcg gcgttaacgc cttatccggc ctacaaagtc ctgcagattc
    24121 aatatattac aggtactgcg taggcctgat aaacgtagcg catcaggcta ttttgcattt
    24181 gtcatcagcc gtcgtcaccc cgacagtatc ggggtgacgg ataatcactt actcgatccc
    24241 tttactacgc aggtaatctt cgtaattacc gctaaagtcg atcacgcgtt ccggggtgat
    24301 ttccagaatg cgggtcgcca gggagcttac gaactcacgg tcgtgtgaaa caaagatcag
    24361 cgtgccctga tacagttcca gcgccatgtt cagtgactcg atggattcca tatccaggtg
    24421 gttggtcggt tcgtccatga tcagaatgtt cggcttctgc atcattaact taccaaacag
    24481 catccgccct ttttccccac cggaaagcac tttagctggc tttttgatgt cgtcctggct
    24541 gaacagcaaa cgaccgagaa tgctgcgtac cgcctgctcg tcatcgcctt cctgcttcca
    24601 ctgactcatc cattcgaaca cggtcagatc attttcaaac tcatattcgt ggtcctgggc
    24661 gtagtaacca atgcgcgcgt tttcagacca ttttacggtg ccgctgtccg gttgcagatc
    24721 gcccaccagc gttttcagca gcgttgattt accgacgccg ttggtaccca gtaccgcaag
    24781 tttttcaccg acctccagca gcaggttgag atttttaaac agtggaccgt tatcaaaccc
    24841 tttggtcaga ccttccactt ccagcgcgtt acggaacagt ttcttatcct gttcaaaacg
    24901 gataaacggg ttctgacggc tggaggcttt cacctcttcc agtttgattt tatcaatctg
    24961 gcgcgcgcgc gaagttgcct ggcgagattt cgaggcgttg gcgctaaagc ggctaacgaa
    25021 agattgcaac tcagcaatct gcgctttctt cttggcgtta tcggccagca gacgttcacg
    25081 cgcctgggtc gccgccgtca tgtactcatc gtagttaccc ggataaacgc gcagctcgcc
    25141 gtaatccaga tccgccatgt gggtacagac catgttaagg aagtgacggt cgtgcgagat
    25201 gatgatcatg gtgctgtcac gctcgttcag cacctgttcc agccagcgaa tggtgtcgat
    25261 gtcgaggttg ttggtcggtt cgtcgagcag gagaatatcc ggctcagcaa acagcgcctg
    25321 cgccagaagc acacgcagct tccagccagg agcaacttca ctcatcgggc cgtagtgctg
    25381 ttccactgga attcccacgc caagcaacag ttcaccggcg cgagcttccg cagagtaacc
    25441 gtccatttcg ccgtatttca cttccagatc ggccacttta tagccgtctt cttcactcat
    25501 ttccggcaaa gcatagatgc ggtcgcgctc ctgcttcact tcccacaact ctttatgccc
    25561 catgatcacc gtatccagca cagtgaactc ttcaaaggca aactgatcct gacgcagctt
    25621 accaatgcgc tcgttgggat cgagggaaac gttacccagc gtcggctcaa ggtcgccgcc
    25681 gagaatcttc ataaaggtgg atttaccact accgttcgcg ccaatcaggc cgtaacggtt
    25741 gccgccgccg aatttgacgg aaatgttttc aaacaacggc ttactgccga actgcatggt
    25801 gacgttactg gaaactaaca caggcgtatc ctgaaaagag atatgacaaa ccgcgcatta
    25861 tgccataagt ctgaaagaag atcctgctcc tcgcttaagg tctacactca agccaaaggc
    25921 aaaaaatgtg atttcgtaca catctgattt cactgtgagc tggaatgaac ttataatgcg
    25981 cttccaatac tctaatattc tcaacccaat ggcctgccag gcacaaaatc tcgcttaaca
    26041 tgaatatgaa attgaaaaca ttattcgcag cggccttcgc tgttgtcggc ttttgcagta
    26101 ccgcctctgc ggtaacttat cctctgccaa ccgacgggag tcgcctggtt ggtcagaatc
    26161 aggtgatcac cattcctgaa ggtaacactc agccgctgga gtattttgcc gcggagtacc
    26221 agatggggct ttccaatatg atggaagcga acccgggtgt ggataccttc ctgccgaaag
    26281 gcggtactgt actgaacatt ccgcagcagc tgatcctgcc ggataccgtt catgaaggca
    26341 tcgtcattaa cagtgcagag atgcgtctgt attactatcc gaaagggacc aacaccgtta
    26401 tcgtgctgcc gatcggcatt ggtcagttag gcaaagatac gcctatcaac tggaccacca
    26461 aagttgagca taagaaagca ggcccgacct ggacgccgac cgccaaaatg cacgcagagt
    26521 accgcgctgc gggcgaaccg ctaccggctg tcgttccggc aggtccggat aacccgatgg
    26581 gtctgtatgc actctacatc ggtcgcctgt atgctatcca tggcaccaac gccaacttcg
    26641 gtatcggcct gcgtgtaagt catggttgtg tgcgtctgcg taacgaagac atcaaattcc
    26701 tgttcgagaa agtaccggtc ggtacccgcg tacagtttat tgatgagccg gtaaaagcga
    26761 ccaccgagcc agacggcagc cgttatattg aagtccataa cccgctgtct accaccgaag
    26821 cccagtttga aggtcaggaa atcgtgccaa ttaccctgac gaagagcgtg cagacagtga
    26881 ccggtcagcc agatgttgac caggttgttc ttgatgaagc gatcaaaaat cgttccggga
    26941 tgccggttcg cctgaattaa tcgtcaaaac gtaaaacaaa ggcggactca taatccgcct
    27001 tttttatttg ccagacctta ccaggccggg atcataacta acaaaacgta tcccaccagc
    27061 gccgcccaca acagcatcgg tattgaatag agatggaaac gccaccagat gcggcgatcg
    27121 ttcgccatac gtagcgcaat caaatttgcc agcgatcctg gtaataaacc aaagcccccc
    27181 acgtttaccg cccatgccag taataaagac ggaggtacat aattgagcag caaaatggta
    27241 ctcggcacgt tactgatcac ctgcgataaa ccgactgccg ttaaccataa cccgggttca
    27301 gataaatgac tcacgttacc caacacgcct tgcaacgctg gaagctgggt cagtaaatgg
    27361 acgtcgataa acatcgccat aaacaccagc agcaacgtcc agtccacact caacaccacg
    27421 cgacgcgcca gcagcacaaa gcccgccgcc acaatcacca gtccccacag ctcttgtttg
    27481 aactccagcg ccgtcagaaa gacgatatac agccccagac aattccacac cagccgcggt
    27541 ttccactccg gtgtttgcac ccccgtatgg tattgcagcg cctttccagg gaaacaacac
    27601 cagcacaaca gcaggagtgt cagcatcatt gcgccagcca tcggtgccat ttgggcaata
    27661 aatccggcaa acgaaagacc agaacgtccc cagataagaa tattttgcgg gttgccaatt
    27721 ggcgtcagta gcgaaccagc gttgactgcc agcgcctcaa aaataatcag ccgattaacc
    27781 gggatctcac acagtctttt tagcgtgata gtcagcggaa caacaataaa cagcgcgaca
    27841 tcgttggtca gaaaggtaga aagcagcgcc gccgccagca ccataaacat cgccagccga
    27901 cgctccgtag caaagcggcg caccattttg cgccccagca catcaaaata accgcttaac
    27961 tccacacctt tggtcagcag catcaggccg cttaaggtga tgatggtgtg ccagtcgata
    28021 gcagcaggcc aggatttcgg tgcaaagggc acgaaaaagc ttaatccgat accaacaaga
    28081 attaataact gaaaaaaacg atcgccttgc agcgtgcgta aaaaaggcag gctcatttgg
    28141 caccgtgttt ctgggtaaac aaacgaaaag cgtcgagcgt ctcttcacta acatggtgct
    28201 ccatgccttc cgcgtcgcga cgggcgattt ccggactgac gcccaacacc aacaagaaat
    28261 tttcgactat ctgatgtcgc tcgcggcttt cctgcgccag cttctctcct tctgccgtta
    28321 aaaacacgcc acgccagggg atcatttcaa tcagccccat ggttgccagc cgcttaagca
    28381 ttttagccac cgtcggttgc gaaactccca gacgagcagc catgtccacc tgacgagctt
    28441 cccccacttc cctgatcaag tcagaaatca gctcaacgta atcatcaata agctcgcgcc
    28501 gatgcgcctc tctgacctgg cggaaccctt caacgtgctc ttccacgttc actaactgcg
    28561 tcactttttt tgctattggc gtacctgcgc gacgactcat tgtgcttcct cattatggtg
    28621 acgcatacat agcgttctca tttcgtgcgc acattgtaaa ccagagttgc gaaggtacaa
    28681 aaaattaacg ttttagcaat agctatataa tatagcctgt gctatatctg tatgtaatgc
    28741 aatcatccct caaggatcga cgggattagc aagtcaggag gtcttatgaa tgagttcaag
    28801 aggtgtatgc gcgtgtttag tcattctccc tttaaagtac ggttaatgct gctctctatg
    28861 ttgtgcgata tggtcaacaa caaaccgcag caagataaac cttcagataa atagcggcgt
    28921 cgcggtacgc cgcttcactc ctgcttttat gcaggcataa cgcgttttgg tctgaaaaaa
    28981 accacttttt gtcggatttg caatcccctt cgcaaaagat ttgttcgtca gtagttgacc
    29041 tgaacggcgg ctcgctctat cttcttgcag ccctgcgtat attgcggctc gcggatgcgg
    29101 acccctttcc cctcttcacg cactcttgca ggtattgacc cttgacgcca gggtaagcac
    29161 atggcgtttg ttacgatagt ggcatatgaa tttaaccctc aaagaatcgc ttgttacccg
    29221 tagccgggta tttagcccgt ggactgcgtt ctacttttta cagtcgctat taattaacct
    29281 cggcttaggt taccccttca gtttgctcta caccgctgcg tttacggcta ttttgctttt
    29341 gctatggcga acattgcctc gcgtacaaaa agttctggtc ggtgtcagtt cgctggtggc
    29401 ggcttgttat ttcccttttg ctcaggccta cggcgcgcct aatttcaata cattgctggc
    29461 attgcactcc accaatatgg aagagtcgac cgaaatcctg acgatttttc cgtggtacag
    29521 ctacctggtc ggcttattta tttttgcgct cggcgtaata gcaatcaggc gaaaaaaaga
    29581 gaatgaaaaa gcgcgctgga ataccttcga cagcctgtgt ctggtattca gtgtggcgac
    29641 attttttgtt gctcccgtgc aaaacctggc ctggggtggc gtatttaaac tgaaagatac
    29701 tggctatccg gtatttcgtt ttgctaagga tgtcatcgtc aataataacg aggtgattga
    29761 agagcaagaa cggatggcaa aactttccgg aatgaaagat acctggacgg tcactgccgt
    29821 taagccgaag tatcagacct atgtggtagt gatcggtgaa agcgcgcgtc gcgatgccct
    29881 cggtgccttt ggcggtcact gggacaatac cccgtttgcc agcagcgtta acggtttgat
    29941 atttgctgac tacattgctg ccagtggctc cacgcagaaa tcgcttggct taacgctcaa
    30001 tcgcgtagtc gatggcaaac cacagtttca ggataacttt gtcaccctgg caaatcgcgc
    30061 gggcttccag acctggtggt tttccaacca gggccaaatc ggcgaatacg atactgctat
    30121 cgccagcatc gccaaacgtg cagatgaagt gtacttcctg aaagaaggta attttgaagc
    30181 agataaaaac acgaaagacg aagcgttact ggatatgacc gctcaagtgc tggcgcaaga
    30241 gcactcgcaa ccgcagctga ttgttctgca tctgatgggc tcgcatccgc aggcctgcga
    30301 caggacacaa ggcaaatacg aaacctttgt gcaatcgaaa gaaacgtcgt gctatctcta
    30361 taccatgacg caaacggacg atttactgcg caagctgtac gatcagttac gcaacagcgg
    30421 cagcagcttc tctctggttt acttttctga ccacggtctg gcctttaaag agcgcggcaa
    30481 agacgtgcaa taccttgccc atgatgataa atatcagcaa aatttccagg tgccttttat
    30541 ggtcatttcc agcggcgata aagcgcatcg ggtgattaaa gcccgccgct cagccaatga
    30601 cttcttaggc tttttctcgc agtggacggg aattaaagcg aaggaaataa atatcaaata
    30661 cccgtttata tctgagaaga aagccgggcc gatatacatc accaacttcc agttacagaa
    30721 ggtggattac aaccatctcg gaaccgatat tttcgacccg aaaccttaaa aacaaaaatc
    30781 cgccccgaga ggcggatttt tatatcacca aagtgattag aagcggtaac caacaccggc
    30841 aatccaagtg cctacgtcaa cgctacgaat acggctctgc tcgtaagaga agtccagagc
    30901 aacgttttcc atcgggttga actgcagacc agcaccgtag gagaaaccgt agtcgctggt
    30961 gtcgtgtttg taggtcgggt attcagtggt ctggaattta ccataaccca cacccactac
    31021 accgtagatg cttgcccagt cgttaatgcg gtaagccgga ccagcagtga tgccgtagta
    31081 ctggtttttg ttgtagtcac cagagcttgc agtacggctt ttctcggtgt aagtgaaaga
    31141 accgatcaca cccagcgggc tgttgtcttc ttcatagcgg tatttcaggt tgaaaccgcc
    31201 cattttgttc atttggccct gagcgtcgct ctgtgcgtaa ccgccagtta cggtagaagt
    31261 cgcagctacg gaagtacctg cggtgaaagc cagaactgcg gccagtgctg aaagacatgc
    31321 aatttttttc ataaccacct caaatgtgat tcaaataagt cctaagtttt aaatatatca
    31381 aaaattaatg ggaaactctt cgcgatttgt gatgtctaac gggccatttc atgtaacaga
    31441 acgtttccat acaccgctat ccatctaaat ttaaatcact ttttcagaga actgcgtaag
    31501 tattacgcat gttttccctg tcattcatcc agattattcc taatcaccag actaatgatt
    31561 ccatcaatcc tggcgcattt tagtcaaaac gggggaaaat tttttcaaca aatgctcaac
    31621 cagcattggg tatatccagt acactccacg ctttacttaa gtctagatat ttgtgggaga
    31681 aaggatgcct ggttcattac gtaaaatgcc ggtctggtta ccaatagtca tattgctcgt
    31741 tgccatggcg tctattcagg gtggagcctc gttagctaag tcactttttc ctctggtgag
    31801 cgcaccgggt gtcactgcgc tgcgtctggc attaggaacg ctgatcctca tcgcgttctt
    31861 taagccatgg cgactgcgct ttgccaaaga gcaacggtta ccgctgttgt tttacggcgt
    31921 ttcgctgggt gggatgaatt atctttttta tctttctatt cagacagtac cgctgggtat
    31981 tgcggtggcg ctggagttca ccggaccact ggcggtggca ctgttctctt ctcgtcgccc
    32041 ggtagatttc gtctgggttg tgctggcggt tcttggtctg tggttcctgc taccgctggg
    32101 gcaagacgtt tcccatgtcg atttaaccgg ctgtgcgctg gcactggggg ccggggcttg
    32161 ttgggctatt tacattttaa gtgggcaacg cgcaggagcg gaacatggcc ctgcgacggt
    32221 agcaattggt tcgttgattg cagcgttaat tttcgtgcca attggagcgc ttcaggctgg
    32281 tgaagcactc tggcactggt cggttattcc attgggtctg gctgtcgcta ttctctcgac
    32341 cgctctgcct tattcgctgg aaatgattgc cctcacccgt ttgccaacac ggacatttgg
    32401 tacgctgatg agcatggaac cggcgctggc tgccgtttcc gggatgattt tcctcggaga
    32461 aacactgaca cccatacagc tgctggcgct cggcgctatc atcgccgctt caatggggtc
    32521 tacgctgaca gtacgcaaag agagcaaaat aaaagaatta gaca
//
LOCUS       DAJTQY010000039        32131 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.039, whole genome shotgun sequence.
ACCESSION   DAJTQY010000039 DAJTQY010000000
VERSION     DAJTQY010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32131
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.039"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(89..625)
                     /gene="ssb1"
                     /locus_tag="OO349_001901"
     CDS             complement(89..625)
                     /gene="ssb1"
                     /locus_tag="OO349_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HCR5866991.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            879..3701
                     /gene="uvrA"
                     /locus_tag="OO349_001902"
     CDS             879..3701
                     /gene="uvrA"
                     /locus_tag="OO349_001902"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HCR5866992.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(3736..4092)
                     /locus_tag="OO349_001903"
     CDS             complement(3736..4092)
                     /locus_tag="OO349_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCR5866993.1"
                     /translation="MTISELLQYCMAKPSAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(4096..4512)
                     /locus_tag="OO349_001904"
     CDS             complement(4096..4512)
                     /locus_tag="OO349_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HCR5866994.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMECFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(4623..5336)
                     /gene="aphA"
                     /locus_tag="OO349_001905"
     CDS             complement(4623..5336)
                     /gene="aphA"
                     /locus_tag="OO349_001905"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HCR5866995.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            5738..5942
                     /locus_tag="OO349_001906"
                     /pseudo
     CDS             5738..5942
                     /locus_tag="OO349_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165333.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6445..7638)
                     /gene="tyrB"
                     /locus_tag="OO349_001907"
     CDS             complement(6445..7638)
                     /gene="tyrB"
                     /locus_tag="OO349_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HCR5866996.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEVTNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(7891..8970)
                     /gene="alr"
                     /locus_tag="OO349_001908"
     CDS             complement(7891..8970)
                     /gene="alr"
                     /locus_tag="OO349_001908"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HCR5866997.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(9023..10438)
                     /gene="dnaB"
                     /locus_tag="OO349_001909"
     CDS             complement(9023..10438)
                     /gene="dnaB"
                     /locus_tag="OO349_001909"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCR5866998.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            10521..11504
                     /locus_tag="OO349_001910"
     CDS             10521..11504
                     /locus_tag="OO349_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HCR5866999.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDHVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGIGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(11670..11912)
                     /gene="pspG"
                     /locus_tag="OO349_001911"
     CDS             complement(11670..11912)
                     /gene="pspG"
                     /locus_tag="OO349_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HCR5867000.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKMPKYQRYDRWRY"
     gene            complement(12046..13083)
                     /gene="dusA"
                     /locus_tag="OO349_001912"
     CDS             complement(12046..13083)
                     /gene="dusA"
                     /locus_tag="OO349_001912"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HCR5867001.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIEHELSQGTYLGHIIRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            complement(13446..14431)
                     /locus_tag="OO349_001913"
                     /pseudo
     CDS             complement(13446..14431)
                     /locus_tag="OO349_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 domain-containing protein"
     gene            14749..15264
                     /gene="zur"
                     /locus_tag="OO349_001914"
     CDS             14749..15264
                     /gene="zur"
                     /locus_tag="OO349_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HCR5867002.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            complement(15306..15515)
                     /locus_tag="OO349_001915"
     CDS             complement(15306..15515)
                     /locus_tag="OO349_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCR5867003.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(15631..16956)
                     /gene="dinF"
                     /locus_tag="OO349_001916"
     CDS             complement(15631..16956)
                     /gene="dinF"
                     /locus_tag="OO349_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HCR5867004.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            complement(17029..17637)
                     /gene="lexA"
                     /locus_tag="OO349_001917"
     CDS             complement(17029..17637)
                     /gene="lexA"
                     /locus_tag="OO349_001917"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HCR5867005.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            complement(17747..18115)
                     /gene="dgkA"
                     /locus_tag="OO349_001918"
     CDS             complement(17747..18115)
                     /gene="dgkA"
                     /locus_tag="OO349_001918"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCR5867006.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            18286..20709
                     /gene="plsB"
                     /locus_tag="OO349_001919"
     CDS             18286..20709
                     /gene="plsB"
                     /locus_tag="OO349_001919"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HCR5867007.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RFYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            complement(20864..21736)
                     /gene="ubiA"
                     /locus_tag="OO349_001920"
     CDS             complement(20864..21736)
                     /gene="ubiA"
                     /locus_tag="OO349_001920"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HCR5867008.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWIMAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(21749..22246)
                     /gene="ubiC"
                     /locus_tag="OO349_001921"
     CDS             complement(21749..22246)
                     /gene="ubiC"
                     /locus_tag="OO349_001921"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HCR5867009.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            complement(22469..24049)
                     /locus_tag="OO349_001922"
                     /pseudo
     CDS             complement(22469..24049)
                     /locus_tag="OO349_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
     gene            complement(24278..25198)
                     /gene="malM"
                     /locus_tag="OO349_001923"
     CDS             complement(24278..25198)
                     /gene="malM"
                     /locus_tag="OO349_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HCR5867010.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELPLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQELGVMSAERLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            complement(25350..26690)
                     /gene="lamB"
                     /locus_tag="OO349_001924"
     CDS             complement(25350..26690)
                     /gene="lamB"
                     /locus_tag="OO349_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HCR5867011.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            complement(26762..27877)
                     /gene="malK"
                     /locus_tag="OO349_001925"
     CDS             complement(26762..27877)
                     /gene="malK"
                     /locus_tag="OO349_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HCR5867012.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIYIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            28242..29432
                     /gene="malE"
                     /locus_tag="OO349_001926"
     CDS             28242..29432
                     /gene="malE"
                     /locus_tag="OO349_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HCR5867013.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSTGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            29586..31130
                     /gene="malF"
                     /locus_tag="OO349_001927"
     CDS             29586..31130
                     /gene="malF"
                     /locus_tag="OO349_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HCR5867014.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYVWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            31145..32035
                     /gene="malG"
                     /locus_tag="OO349_001928"
     CDS             31145..32035
                     /gene="malG"
                     /locus_tag="OO349_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HCR5867015.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
BASE COUNT         8172 a         8248 c         7782 g         7929 t
ORIGIN      
        1 cctccataac ggaggcttct tttttatggc tggcagatgc tttaatcatc caccttaaaa
       61 caatataacc tattgtttta atgacaaatc agaacggaat gtcatcatca aagtccatcg
      121 gcggctcgtt agacggcgct gccggagcgg actgctgcgg gcgagactgc gcgccgccgc
      181 tgaactgatt gccaccctgc ggctgctgag gttgacccca accgccctgc ggctgaccac
      241 caccgatatt gccacctgcc ggagcgccac caccctgacg accacccagc atctgcatgg
      301 tgccgccaac gttcaccacg acttctgtgg tgtagcgatc ctgaccggat tgatcggtcc
      361 atttacgggt acgcagctga ccttcgatat aaacctgaga acctttacgc agatattcgc
      421 tcgccacttc tgccagtttg ccgaacagca caacgcggtg ccattcagtc tgttctttca
      481 tctcgccggt cgctttatca cgccaggatt cggaagtagc cagcgtaatg ttggcaactg
      541 cgccaccatt tggcatgtag cgtacttccg ggtcctgacc cagattacca acgagaataa
      601 ccttgtttac gcctctgctg gccatgttcg tgtctcctga aaaaaatcgt tctgaataag
      661 tgtaaacgcg cgattgtacc attaccaata gcgcttttac tatgttgtga cctcggttcc
      721 gggaaacaaa cctggccaga cattgttaca caacactccg gataatgcat tccaatactg
      781 tatattcatt caggtcaatt tgtgtcataa ttaaccgttt gtgatcgccg gtagcaccat
      841 gccaccgggc aaaaaagcgt ttaatccggg aaaggtgaat ggataagatc gaagttcggg
      901 gcgcccgcac ccataatctc aaaaacatca acctcgttat cccccgcgac aagctcattg
      961 tcgtgaccgg gctttcgggt tctggcaaat cctcgctcgc tttcgacacc ttatatgccg
     1021 aagggcagcg ccgttacgtt gaatcccttt ccgcctacgc gcggcagttt ctgtcactga
     1081 tggaaaagcc ggacgtcgat catattgagg ggctttctcc tgccatctca attgagcaga
     1141 aatcgacgtc tcataacccg cgttctacgg tggggacaat caccgaaatc cacgactatt
     1201 tgcgtttgtt gttcgcccgc gtcggcgaac cgcgctgtcc ggaccacgac gtcccgctgg
     1261 cggcgcaaac cgtcagccag atggtggata acgtgctgtc gcagccggaa ggcaagcgtc
     1321 tgatgctgct cgcgccaatc attaaagagc gcaaaggcga acacaccaaa acgctggaga
     1381 acctggcaag ccagggttac atccgtgctc gtattgatgg cgaagtctgc gatctttccg
     1441 atccgccgaa actggaactg caaaagaaac ataccattga agtggtggtt gatcgcttca
     1501 aggtgcgtga cgatcttacc caacgtcttg cggagtcgtt tgaaaccgcg ctggaacttt
     1561 ccggtggtac agcggtagtg gcggatatgg acgacccgaa agcggaagag ctgctgttct
     1621 ccgccaactt cgcctgccca atttgcggct acagtatgcg tgaactggag ccgcgactgt
     1681 tttcgtttaa caacccggca ggtgcctgcc cgacctgtga cggccttggc gtacagcaat
     1741 atttcgatcc tgaccgcgtg atccaaaacc ccgagctgtc actggctggc ggtgcgatcc
     1801 gtggctggga tcgccgcaac ttctattact tccagatgct gaaatcgctg gcagatcact
     1861 ataagttcga cgtcgaagcg ccgtggggca gcctgagcgc gaacgtgcat aaagtggtgt
     1921 tgtacggttc tggcaaagaa aacattgaat tcaaatacat gaacgatcgt ggcgatacct
     1981 ccatccgtcg tcatccgttc gaaggcgtgc tgcacaatat ggagcgccgt tataaagaga
     2041 cagaatccag tgcggtacgt gaagaattag ccaagtttat cagcaatcgc ccatgcgcca
     2101 gctgcgaagg gacgcgtctg cgtcgggaag cgcgccacgt ttatgtcgag aatacgccgc
     2161 tgcctgctat ctccgacatg agcatcggtc atgcgatgga attcttcaac aatctcaaac
     2221 tcgctggtca gcgggcgaag attgcggaaa aaattcttaa agagatcggc gatcgcctga
     2281 aattcctcgt aaacgtcggc ctgaattacc tgacactttc ccgctcggca gaaacacttt
     2341 ccggcggtga agcccagcgt atccgtctgg cgagccagat tggtgcgggc ctggttggcg
     2401 ttatgtacgt gctggacgag ccgtctatcg gcctgcacca gcgcgataac gagcgcctgt
     2461 tgggtacgct tatccatctg cgcgatctcg gtaataccgt gattgtggtg gagcatgacg
     2521 aagacgcaat tcgcgccgct gaccatgtga tcgatatcgg tcctggtgcg ggtgttcacg
     2581 gcggtgaagt ggtcgcagaa ggtccgctgg aagcgattat ggcggtgcct gaatcgttga
     2641 ccgggcagta catgagcggt aaacgcaaga ttgaagtgcc gaagaaacgc gttccggcga
     2701 atccagaaaa agtgctgaag ctgacaggcg cacgcggcaa caacctgaag gacgtgacgc
     2761 tcacgctgcc agtcggtctg tttacctgca tcacaggggt ttcaggttcc ggtaaatcga
     2821 cgctgattaa cgacacactg ttcccgattg cccaacgcca gttgaatggt gcgaccatcg
     2881 ccgaaccggc accgtatcgc gatattcagg gactggagca tttcgataaa gtgatcgata
     2941 tcgaccaaag cccaattggt cgtactccac gttctaaccc ggcgacctat accggcgtgt
     3001 ttacgcctgt gcgcgaactg tttgcgggcg taccggaatc ccgtgcgcgt ggttatacgc
     3061 caggacgttt cagctttaac gtccgtggcg ggcgctgcga agcctgtcag ggcgacggtg
     3121 tgatcaaagt ggagatgcac ttcctgccgg atatctacgt gccgtgcgat cagtgcaaag
     3181 gtaaacgcta taaccgtgaa acgctggaaa ttaagtacaa aggcaaaacc atccacgaag
     3241 tgctggatat gaccatcgaa gaggcgcgtg agttctttga tgcggtgcca gctctggcgc
     3301 gtaagctgca aacgttgatg gacgttggtc tgacgtacat tcgcctgggg cagtccgcaa
     3361 ccacactttc tggtggtgaa gcccagcgcg tgaagctggc gcgtgagctg tcaaaacgcg
     3421 gcaccgggca gacgctgtat attctcgacg agccgaccac cggtctgcac ttcgccgata
     3481 ttcagcaact cctcgacgtg ctgcataaac tgcgcgatca gggcaatacc attgtggtga
     3541 ttgagcacaa tctcgacgtg atcaaaaccg ctgactggat tgtcgacctg ggaccagaag
     3601 gcggcagtgg cggcggcgaa atcctcgtct ccggtacgcc agaaaccgtc gcggagtgcg
     3661 aagcttcgca tacggcacgc ttcctcaagc cgatgctgta atcgttaagg ccgctttctg
     3721 agcggccttt tcctttcaga gttgtaccag caatttacgc ttttcttccg gtagtaaatt
     3781 cactgcctgc tgataagacg catccaccag ataatagatt tgcgaatccg gcagcgaacc
     3841 gtcgagatac acggtgctcc agtgcgcttt attcagatgg cggctaggac gcacatcgct
     3901 gtgctgctga cgtagcagct ccgccagctc cgggctggtt ttcagcgaaa cagctgggcg
     3961 attttctact tctttcacca tcgcaaacag tacatcttcc actttgatct gcgtcgcttt
     4021 ccagtcgtta tgcacgctct gttctgcgct tggttttgcc atgcaatatt gtagcaactc
     4081 cgaaatggtc attttttact ccccttgtag tgtcgcgatg atgcgacgcg atccgccgtg
     4141 gatgcgatgt tcccccagcc agatgccttg ccaagtgccg gtctgaatac gccctttatg
     4201 taccggcaat acaagcgatg ttcccagcat tgaggatttg atatgagaag gcatatcgtc
     4261 tgctccctca tagtcatgct cataatttcc gttgtcggga acggtgcgga ggaaaaaaca
     4321 ctccatgtcg tggcgtacgg tgggatcgca gttctcatta agtgtcagag aggcggaggt
     4381 atgttgcagc aacagatgca gtaagccgat gttaacgcgc ggcatatcag ccagctgatt
     4441 cagaatttca tccgttacca gatgaaaccc acgagatttg gcgctaagcg tgagcgtctt
     4501 ttgataccac atgtgctgct ccttgataaa actctcttaa tcagtttgca gcaagacagc
     4561 gaaaggataa aggtgtgatt aaaaaaacag cattcgggag agcatcactc tctcccgctc
     4621 tgtcagtatt ctgaattgac gatcacctct tcaccaaacg cacccgcttg tggcaagggt
     4681 ttgtaggtag agttggaggc gcgcagaatg cggataccac gagcgccgac atcgcgtgcg
     4741 gcggtaatat cgttatcgga atcgccataa aaaattcgga tatttttatc ctgcagccat
     4801 tgcgattttg tattttgccc tggtttatcg cccgcaaaga ttaccggatt catgttggtg
     4861 gcaggaatat gaaaattatc cgccagcgtt tttgaaaccg tttctgtttt cgtcgggcta
     4921 cgaccagtca caaagaagat cgcgtcaccg cggcgtacat gcatatcaat cagctggcga
     4981 gcgacctctt ttggaatgct gaattcatcc cagccattat tcattttttc ccagaacaca
     5041 ggatttttca gataatcttc gctttctggc gagaaggttt ttttgccgcg ccagaatccc
     5101 ggactggaaa aaagtaccgt gtcatcgata tcaaacccca ccgccattgg cggacgccct
     5161 gcgaggctat tttcaatttg tgcgaccgaa acccaatgaa ttggtgcctg ttcagcaagc
     5221 ctggcaacgt tagtcccagg gttaagcggt gaaggagatg aggccagggc aacagcggaa
     5281 ctgtttagag cgaacaataa gcaaacggca ctgattgcct gtgtgatctt gcgcatattt
     5341 ttccctaaat agtcagtatg ttgaaacttt ttattgtgag atttgttgca aaaaccatct
     5401 gaccataacg ccagctgccg gggaaaggaa ggattatctg cggtttttgt gatgaaaagc
     5461 aggatgatta tatttaaccc agtaaatacg ttcctgtctt tttttgtata aaaaatgttt
     5521 attactcgat gtaaggatac attaatttta cgtttattat gatgaatcct cggcaaatga
     5581 gcaaaatttg atagaacatt tatctgctgt acgatttaat gaacttaatg atacagcatt
     5641 acaaaaacaa tcgaagttta taaagatgat ttctgattga ccagccccct accctacaat
     5701 ggtgattaat tttattactc gcgatggaga tgatgatatg aatatatcat atgtgaatag
     5761 caacaaaacg acatctttac ctgttgagct agatgcacta aataataaag atatctctta
     5821 tgccaaagat ttctttttat acatagaaac acagttaaaa attgcaaaag atttttgtag
     5881 acctggagaa gaagtatcaa gttctattgc aagtaaagtt tttcacgcat ttattgattt
     5941 agttaacaaa atcaggggta agaaagactt tatgtatatt tgcacgcttt gctgttttgc
     6001 cgaggaggtt aaaggtgatt attctcatta caggaccttt ttatttgata ttggtaatca
     6061 atataaggtt aaacttacac aaagcggaaa aaagagttct ctttaacatt agaatttaac
     6121 gatactataa ttgaatctca gaaagtcaca ggcaataagg caaagcatat tcttgaggat
     6181 atagaaatat tctatcgtaa caaacccgat acttattatt aaatttaata atggttagat
     6241 gtgcagaaat tattactcca cacgaaaaaa tagggaaagg aattccagat aattgggata
     6301 aacttaacaa taaaagaact gacaacgttt tattatctct cacgtagaac aacggcatca
     6361 aaaaaatcat ctacaaagaa atagtgaaag cgacgacagt ttaatttcac tgcaggctgg
     6421 gtaactccag cctgctttcc tgcattacat caccgcagca aacgcctttg ccacacgttg
     6481 tacatttgcc gtatttaacc cggcgacaca catgcgaccg ctggcgatga gatagacacc
     6541 aaattcttca cgtagtcggt caacctgagc ggcacttaaa ccggtataac tgaacatgcc
     6601 gcgctgatta agcagataat cgaaattgcg ttctggcatc tctgtgctta ataccttcac
     6661 caattcctga cgcattgcca gaatgcgagt acgcatctct tctacttccg ccagccagct
     6721 ggctttcaat gcctcgtcat tcagcactgc agccaccacc tgcgcaccaa aattcggcgg
     6781 gctggagtag ttgcggcgaa ctgttgcttt caattgcccc agtacgcggc cagcggcttc
     6841 ggcatcttca cacagaacag aaagtccgcc gacgcgctcg ccgtaaaggg agaaaatttt
     6901 cgagaacgaa ttgctcacca gagcgggtaa tccagcgctg gcaatggcgc gaatagcgta
     6961 ggcatcctct tccataccgg cactaaatcc ttgataggca atatcgagga atgggataag
     7021 ctcgcgggct ttgagaattt caatcaccgc atcccactga tcattagtga gatcggcacc
     7081 cgttgggttg tggcaacatg gatgcagcaa cacaatactg cgggcaggta atgttttcag
     7141 cgtcgccaac aggtcattaa agcgcacgct gttagtcact tcgtcatacc aggggtaagt
     7201 actcacttcg aatccagccc cggcgaatat tgctacgtgg ttttcccaag taggatcgct
     7261 gacccagacg cctgattccg ggaagtagcg tttcaggaaa tccgcgccca ctttcaatgc
     7321 ccctgagccg ccaagggttt gaatggttgc tacgcgctgt tgttgcagta ccggatggtc
     7381 cgcaccaaac agcagcggcg caatggcatg gcgatagctg ttaagccctt ccatcggtaa
     7441 ataaagcgaa gcgccatggg gctgcgcatt caggcgcgct tccgcctccg ccacggcttt
     7501 caattgtgga ataattccgt cttcgttgta gtacagaccg atacttaaat tcactttgtc
     7561 gctgcgaggg tcttctttaa aacgctccat aagcgtaaga atcgggtcgc cagcgtaggc
     7621 gtcaactttt tgaaacacgc gatggttctc caggtttacg ggcaggtggt taaaacacaa
     7681 taaaccggaa gaaggcgaag atcgagtgga tgttcaggag cgaacggcaa ttagcaacag
     7741 agtgagacta atgataaacg tacatccgcc agaggcacga cctttataag agcatgaaag
     7801 aacatcaact tattgaattt ttaggatttt attggccgga taaggcattc acgccgcatc
     7861 cggcacagac aatcaaatat tacagaacga ttaatccacg tatttcatcg cgacccttga
     7921 agtcaggcgc gtaataagtt cgtaagcgct tactttcgtc atttcagcga tacgttctac
     7981 gggcaaacct tcgccccata aaatgaccgg atccccggct ttgtcctgcg cctgtggacc
     8041 taagtctacg cagatcatat ccatcgccac gcgcccgaca atcggtactt cgcgaccgtt
     8101 caccagcact ggcgtaccgg acggcgcggc gcgcggataa ccatcgccat agcccatcgc
     8161 gactacgcca agacgggtat cacgttcgct tacccaggtt ccaccataac caacaggctc
     8221 tccggcttta tgctcacgca cggcaatcag gctggaggtt agtgacatca ctggctgaca
     8281 gccaaaatcg gcaccggtgg agcgatcttc cagcggcgag acgccataaa gaatgatgcc
     8341 cgggcgcacc cagtcaaaat gcgactgtgg ccacagcaga atgccacccg acgcggcaat
     8401 ggaacgttga ccaggtttgc cttcgcaaaa ggtattaaag atagcgagtt gtttctcggt
     8461 tgcgccacat tttggttcat ccgcgcgcgc aaaatggctg acgatattca ccggctgacg
     8521 aacgtttttg cactgggtca ggcgatgata aaacgcctca gcctgttccg gccttacgcc
     8581 cagacggtgc ataccggtat cgagtttcat ccagacggta accggctcgt ccaagctagc
     8641 ctcttccagc gcagccagct gttcttcgtt atgcacggcg gtatgaaaat gttgcgcaga
     8701 aatcgtcggc agatctctgg catcaaaaaa gccttcgagt aacagtacag gtttggtgat
     8761 tccccccgca cgcagtcgca gagcttcttc gagacgggct acgccaaagg cgtcagcatc
     8821 ggggagcgtt cgcgcggtct caagaagacc gtgaccataa gcgttcgctt tcaccaccgc
     8881 aaccatttta ctggcaggtg ccagttcacg aagacgttgc aggttgtgtc gcagagcgcg
     8941 gcggttaatc acaacagttg ccgcttgcat ttgtgttcct tgataattgt ttgctttaat
     9001 tacctaattc ataaaataat tattattcgt cgtcgtactg cggccccgca tagttgtcga
     9061 agcgcgacca ttgaccgtta aaggtcaggc gtaccgtccc gattgggccg ttacgttgtt
     9121 taccgataat aatttccgcg atgcctttta aatcactgtt ttcgtgatac acctcatcac
     9181 gatagataaa catgatcaag tccgcatcct gctcgataga gccagattca cgcaggtcgg
     9241 agttgaccgg acgtttgtcg gcacgttgtt ccagagaacg gttcaactgg gacagcgcca
     9301 ccaccggcac gttcagttct ttcgccagtg ctttcagcga gcgggagatt tctgcaattt
     9361 ccagcgtacg gttatcggaa agcgccggta cgcgcatcag ttgcaggtag tcgatcatga
     9421 taagcccgat gccgccgtgt tcacgggcaa tacggcgtgc gcgggaacgc acttccgttg
     9481 gcgtcaggcc ggaagagtca tcgatataga tattgcgttt ttcgagcaaa atacccatgg
     9541 tgccggaaat gcgcgcccag tcttcatcat cgagctgccc ggtacggatt ttagtctggt
     9601 caacgcgcga cagcgacgcc agagaacgca tcatgatctg ttctgatggc atctccagcg
     9661 agaagataag tactggttta tcctgcaaca ttgccgcgtt ttcgacgagg ttcatcgcaa
     9721 atgttgtttt acccatcgac ggacgcgcgg cgacgatgat caaatccgac ggctgcaagc
     9781 cagcggtttt tttgttgaga tcgtcataac cggtgtttac cccggtaacg ccatcgtgtg
     9841 gctgctgaaa caactgctca atacgcgcca cggttgcgtc gagcacatcg gcgatgttct
     9901 tcggcccttc gtctttgttt gcacgacttt cggcaatttt aaagacgcgg gattcagcaa
     9961 ggtccagcag atcttcgctg gtacgcccct gcggatcaaa accggcttca gcaatctcat
    10021 tcgcaaccga gatcatctca cggacaacgg cacgttcacg cacgatgtcc gcataagcac
    10081 tgatgttcgc cgcacttggc gtattttttg acagctctgc cagataagca aaaccaccga
    10141 cgctatcgag ttgcccctgg cgttccaacg attccgcaag ggtaatcaga tcgataggac
    10201 taccgctttc ctgcaaacgc gccatttcag taaagatatg acggtgtggg cgggtgtaaa
    10261 aatcgtctgc taccacacgc tcggctacat catcccagcg ttcgttatcc agcattaaac
    10321 cgcccaacac cgactgctcc gcttcgatcg agtgcggagg cactttcagc ccggcaactt
    10381 gtggatcgcg ttcgcggggt tcagcctgct gtttgttgaa gggtttattt cctgccatag
    10441 tgaatggagt taccgaaata aagaatgggt cgaaacttta ccatatgaag cagaccctga
    10501 cgatacgttc tggaggacac atggcaacac gaattgaatt tcacaagcac ggtggcccgg
    10561 aagtacttca agccgtagag ttcactcctg ccgatccggc ggagaatgaa atccaggtcg
    10621 aaaataaagc catcggcatc aattttatcg acacgtatat ccgcagcggc ctttacccgc
    10681 cgccatcgct acccagcgga ttaggtaccg aagcggcagg catcgtgagt aaagtcggca
    10741 gtggtgtaaa gcatattaag gctggcgatc atgtagtcta tgcgcagtcg gcgttaggcg
    10801 cttacagctc tgtgcataac attaatgcgg ataaagcggc gattctgcct gcggcaattt
    10861 cttttgagca agctgcggca tccttcctga aaggcttaac ggtttattat ctgctgcgca
    10921 aaacctatga aattaaaccc gatgaacagt tcctgttcca cgcagcggct ggcggcattg
    10981 gcttaattgc ctgccagtgg gcaaaagccc tgggcgcgaa actcatcggc accgtaggaa
    11041 ccgcgcaaaa agcgcagagc gcgctaaaag ccggcgcgtg gcaggttatt aactatcgtg
    11101 aagagaatct ggtcgagcga ttgaaagaga tcaccggcgg taagaaagtg cgcgtagtgt
    11161 acgattccgt gggcagagac acctgggaac ggtcgctgga ttgcctgcaa cgccgcggct
    11221 taatggtcag ttttggcaac tcatcaggtg cggttaccgg tgtgaactta ggcattctca
    11281 atcaaaaagg ctcgttgtat gtgacacgcc cttccctgca aggctatatc accacgcggg
    11341 aggaattaac cgaggccagt aatgaactgt tctctttgat tgccagcggt gtgattaagg
    11401 tcgatgtcgc cgagcagcag aaatatccgc tgaaggatgc gcagcgtgcg catgagattc
    11461 tggaaagccg ggcgacgcaa ggttccagcc tgctgattcc ataaaagaaa tagggcttcc
    11521 acctgggaag ccctttcttt ttatagttcg gctgtatgta gggtacagca cgatgaatct
    11581 gttagaggcg caatagtgac agatttgatt atcaattcct attttgttct aaggataaaa
    11641 ccttaggttg tgatcatccg cacaatccct tagtaacgcc agcggtcata acgctgatat
    11701 ttcggcattt ttggtgcttt aatcgcctta ataacccaca ccaccgcaat cgccagtagt
    11761 aaccacggca gcaacttaat catcaatgcc agcataccgc cgaggaacat aatggccgtc
    11821 gccacaacca gcgcggcgat aatgcccagc aacgaaacgc cggtgaccat cagcatgaca
    11881 aaaaagccaa tcacaaaaag tagttccagc atgatgctct cccaaatatg aaatctcttg
    11941 ctggcattac aagaatcatg ccaaaaataa tctattgatt taacagcaaa acgccccgcg
    12001 acggtgcgca gggcgtggtg aatttgacta ctttttggtg aaaagttaac gcttatccgc
    12061 caccagtttg agcgcgtgtt ccagcacgtt aatgtcagca cccgctttat gggcattttc
    12121 acttaaataa cgccgccact gccgcgcgcc aggaataccc tggaacaaac ccagcatatg
    12181 ccggataata tggccgagat acgtcccctg gctgagttca tgctcaatgt acggatacat
    12241 ggcacgcact actgccaccg gatcggcatc ggtatccgag gaaccaaaga tctcccggtc
    12301 taccgccgcc agaatacccg gattctgata cgcctcgcgc ccgaccatca cgccatccat
    12361 atgttgcaaa tgcgctttag cttcttccag cgacttgata ccaccgttaa tcgacattgt
    12421 cagatgcggg aagtcacgct tcagttgata cacacgcgga taatcgagcg gcgggatttc
    12481 acggttttct ttcgggctta accccgaaag ccaggcttta cgtgcgtgga tgatgaacat
    12541 ctcacactcg cctttgccgg aaacggtatt gatgaaatcg cagagaaatt catagctgtc
    12601 ctggtcgtcg atgccaatac gcgttttcac cgtcaccgga atcgacacca catcgcgcat
    12661 cgctttcacg cagtcggcaa ccagctgcgc attacccatc agacacgcac caaacatgcc
    12721 gttctgcacc cggtcagacg ggcagccgac attcaggttg atctcatcat atccgcgcgc
    12781 ttctgccagc tttgcacact gcgccagcgc cgccggatcg ctaccgccca gttgcaacgc
    12841 taccggatgt tcttcttcac tgtacgccag ataatcacct ttaccgtgaa taatcgcccc
    12901 tgtggtcacc atttcggtat aaagcaacgt attgcgggaa agcagacgca agaaatagcg
    12961 gcaatgtctg tccgtccagt cgagcattgg tgcaacacta aaccgaccac tccagtgaac
    13021 gtcagttttt tcaggcattg cgctggtttg gttgattttt tgcatttcag aattaccgtg
    13081 cattttcaaa tgtagagatt attttatcga tatatcatgg ggttatgtta ttcagcttca
    13141 ctgttcagga ggctcaatag cggggtacta taccataaca acaggaagcg cctgtctcat
    13201 tgcaaaagaa aattgagatc ctctcaaggc atgaagctct cacgaagtga tggaaataat
    13261 cttattagcc gttaactttg ttaaggccaa tgataaacaa tccaggttcg acgataaata
    13321 aaaaatcaca cattaaactc tggtgatata tctccctgct aatagcattg atagagaaaa
    13381 aagaacccaa taaatattgg gtccttttat ataatgcctt ccatactatc gaagaacttc
    13441 acatattatt tctccgattt aaccccgaac aagtcataaa ttaatttaga agtgtctgta
    13501 agtattttaa tctcttcctt tgaggttggg tcaaacgact tcgcgaagct aattaattgt
    13561 ggtgcagcat ctcgcatttt gcttaaaata tcaggatcga gcgttccttc attcaccaga
    13621 tgtgacatct tatccagata gccatcaaaa ttcatttctc ccccatctgc cagacgcttc
    13681 atctctccca ggtacttctt catatcacgt tgatataatt tttcaaactg agctttcagg
    13741 tagctctcat tattttcatt aatatacact gtttctgata aatctgcgta ggcactagaa
    13801 tatgaaagtg aaattaacag cgaagcaaat aattttaagc tgttgtcatc atgttcctgg
    13861 caggcattaa caaacgttaa aaaaccagaa cctattgctt gaaaatgtat attcgctaaa
    13921 ggttcattat ccttagattc ctcataaaat agctgaaccg tggagggaag gccattctta
    13981 gtttctacat caaaagtaca ttgttcaatg ggcattgcat ctttattatc cccaggatta
    14041 catatactat taatcgcgaa atgaaaaata gcccggtcaa caactgatgc aataaagtca
    14101 cgatcgttta aatcctcatc agtgcaatcg tcagtatact tattgaccca cattttgaga
    14161 tcatttatgt gctcaattaa aaacacattt ttttacgtgc atgccataaa ttatgcgtga
    14221 ttcgttgaat aaacaaatct atttttgcaa taattatatc ccgatgacgc agcgctgcat
    14281 aatcaataga tttaccatat atattattta atttaccttt gattgaacct ctattatcca
    14341 atttatcttt ggttgcatct ttattttcga attgatttga attgagtgtt ttattaaagt
    14401 atccagttgc accgggtatt atcttcaaca tgattcatct accttttttg tcgtaccgtg
    14461 tcctccttat aattcttttg ccatctggtc actatcaaaa atcgctcata atttctgaac
    14521 tacaggtatg cgtttaaacc cagttaaata aatagcgagt taacttgaag caatttattt
    14581 aaaaatgaat tattaattat tatcgtgttg ataatggtac gacaaggcaa cacaacacct
    14641 tacctacaaa acagaaaatc aacattacgg caacaataag ggttctcggt gtttttcctg
    14701 gaacatggta aagtaaggac attcttaacc cccactttga ggtgcccgat ggaaaagacc
    14761 acaacgcagg agttattagc gcaggctgaa aaaatctgcg cgcagcgtaa tgtgcgcctg
    14821 accccacagc gcctggaagt gttgcgcctg atgagtctgc aggatggcgc tatcagcgct
    14881 tatgatctgc ttgatttact gcgcgaagct gaaccgcaag ccaagccgcc aacggtttat
    14941 cgcgcgctgg attttctgct tgagcaaggt tttgtgcata aggtggaatc caccaacagt
    15001 tatgtgctct gtcatctgtt cgaccagccc acgcatacgt cagccatgtt tatttgcgat
    15061 cgctgcggcg cggtgaaaga agagtgtgca gaaggcgtgg aagacattat gcatacgctg
    15121 gcggcaaaaa tggggtttgc cctgcggcat aatgtgattg aagcacatgg gctctgtgcg
    15181 gcatgcgtgg aagtggaagc gtgtcgtcat cctgaacagt gccagcatga tcactctgtg
    15241 caggtgaaaa agaaaccgcg ttaagagggc gtacatcctt gtacacgtcg ggcaggaggg
    15301 attaattacc agcgatattc attgcgggtt tcccaatcca cgacctcttt ttctgcctga
    15361 tctttctgat aaccataacg ttcctggatt ttaccgacca gttgatcacg tttaccttca
    15421 atgatcgtca tatcatcatc ggtcagtttg ccccattgct ctttcacttt acctttaaac
    15481 tgtttccagt taccgccggc ttcatcttta ttcataatca agacctcatc gttaggttgt
    15541 gaatgagagt acgttcactt ttcttctgaa cgtgagatta agtgtagtca gcaatttggc
    15601 ttaggataat ttattcagaa tttttaaccg tcacgttgcg gcaaaccagg taccgttgcg
    15661 ccagtgacgc cgccagatag ccgccagaga aagcccgcgc aacgccagaa agacggttaa
    15721 tgccagccac aaaccatgat tacccagcca cggcagcgta aggagcgtca gcgcaaaacc
    15781 tgcggcggcc accgccatac tgttacgcat ttcggcggca cgcgttgcgc ctataaacat
    15841 gccgtccagc agataacacc agacgccaac caacggcaaa atcacctgcc agataagata
    15901 gcggtcagcc agctgctgaa tctgggttaa cgacgtcagc aacgcaatga tgtgttcccc
    15961 tgccagcaaa taaaccaccg aaaacagtaa cgctacaatc cccgactggc ggcacgctgc
    16021 ccgccataca tccagcaact ggctaccgtc gcgcgcaccg tatgcctgac cggagtgcgc
    16081 ttcaaccgcg taggcaaaac catcaagcgc ataggcggta aaggtgagta gcgtcatcag
    16141 aaccgcgtta acagcgataa tgtcactccc cagtcgcgca ccaagtacgg tgatcgcgcc
    16201 gaaacagagt tgcaacaaca gcgaacgcag catgatatcg cggttaagcg ccagcaagcg
    16261 acggaagttt cctcgccagg cagttttcag catttcgccg gaaattccgc gtagtttgag
    16321 gattttacgc accattagca gaccaatcag caatgttgca tattccgcaa taaccgtcgc
    16381 cagcgccgcg ccctgcacgt tcatatgcag ccccatcacc agccagacat ccagcacaat
    16441 gttgaggata ttgccgacca ctaacaaaat tactggcgca cgggcatatt gcacgccgag
    16501 taaccaaccg agtaatacca gattcgccag cgacgccgga gcgcttaacc agcggatttc
    16561 aagaaagcgc cgcgcctgtt ccaggaccgc ttcactaccg ccaacaatat gcagcgccag
    16621 atcgataatc ggcgtacgca acagcgcaat taacgccccg gcccccaacg ccaacagcaa
    16681 cggttgcacc agcgcacggg ctaatgcctg aggatttttg gcaccataag cctgcgcagt
    16741 cagcccggtg gtgctcatgc gtaaaaacag caacagcata aagagaaagc tggtcgccgt
    16801 tgcaccaacc gccacgccgc ccaaataaac cggactatca agatgaccaa ttaccgccgt
    16861 atcgaccagt cccagcaacg gaacggtgat attggagaaa atcatgggta aggcgagatg
    16921 ccagagtgct ttatcagatg aagtgaggaa tgccatgcag acaagcctga tgaagagaga
    16981 tgaaaaacaa accgcgacgc caagcggcat cgcggtctca gagatatgtt acagccagtc
    17041 gccgttgcga ataaccccaa ccgccagccc ttcaatggtg aagctctgct gacgaaggtc
    17101 aacgacaatt ggtttaaact cgctattttc tggcaacagt tcgactttat tgccctgttt
    17161 tttcaggcgc ttaacggtaa cttcgtcatc aatacgtgcg acaacgacct gaccgttacg
    17221 tacatcctga gttttatgca ctgccagcaa gtcgccatcc ataatgccga tatctttcat
    17281 cgacatcccg ctgacgcgca gcaggaaatc agcattcggc ttgaacaagg aaggatcgac
    17341 ctgataatga ccttcaatat gctgttgcgc cagaagcggt tcaccggcag ccacacgacc
    17401 taccagcggc aacccttctt cctcttcctg caacagacga atcccgcgtg atgcgccgga
    17461 aacaatttca ataacgcctt tgcgtgccag cgccttcaga tgttcttcag ccgcgtttgg
    17521 ggaacggaac cccaaacgct gcgcgatttc cgcacgcgtc ggcggcatac ctgtctggct
    17581 gatgtgatca cggatgagat caaacacctc ttgttgcctg gccgttaacg ctttcattcc
    17641 gccccctggg tgtatataca gttatactgt gagtatatac agcaaaaggc gattttggaa
    17701 ccataaactg cacaataaac cagagattta tcgaaatctg gaagggttat ccaaaatgcg
    17761 accataacag gatgcaccag gtaatcacgg cgacgataat ggcaatcagc accgcggcgg
    17821 atcccatatc ttttgcgcgt ccggaaagct catggtattc agagccaatt cggtcaacca
    17881 ctgcttcgat ggcgctattg aggatttcca caatcatcac cagcatcacg gagctgataa
    17941 gcagcacgcg ggtaattgcg tccacatcca gccagcaggc gatgaccacc gccaacaata
    18001 ccgctacgcc ttcctgacgg aatgccgctt cgttgatcca tgcagcgcgt aaacctttcc
    18061 aggaatagcc agcagctttg ataattcggg tgaatccagt tgtattattg gccattgaaa
    18121 gaaccttttt acattatgag cgtcaatatc agtgtaccgt taaatccgat gattcgggtt
    18181 tgcgtaacgc cgcgcgaaac atgagcggat accacagaat ttcccatgac tttctgctat
    18241 ccttgccgcg catttgcatt attaaccaga ggctttacat cgtttatgtc cggctggcca
    18301 cgaatttact acaaattact gaatttacca ttaagcatcc tggtaaaaag caagtctatt
    18361 ccggcagatc ctgccccgga actggggctg gatacctctc gtccaattat gtacgtttta
    18421 ccgtacaact cgaaagcaga tttgctgacg ttgcgcgccc agtgtctggc acatgatttg
    18481 cctgacccgt tagagccgct ggaaatcgac ggcacgctac tgccgcgcta tgtgttcatt
    18541 cacggcgggc cgcgtgtatt cacctattac acgccgaaag aagagtctat taagcttttc
    18601 cacgactatc tcgatttgca ccgtagcaac ccaaatctgg atgtgcagat ggtgccagta
    18661 tcggtgatgt ttggtcgcgc gccggggcgt gaaaaaggcg aagtgaaccc gccgctgcgt
    18721 atgcttaacg gcgtacagaa atttttcgct gtactgtggc tcggtcgcga cagttttgtg
    18781 cgtttctcgc cgtcagtgtc gctgcgccgt atggcggatg aacacggcac ggataaaact
    18841 atcgctcaga aactggcgcg cgtggcgcgt atgcactttg cccgtcaacg tctggctgcc
    18901 gtaggcccac gtctgcctgc tcgtcaggat ctgtttaata agctgctcgc ctcccgcgcc
    18961 attgccaaag cggtagaaga tgaagcgcgc agcaaaaaaa tctcccatga aaaagcgcag
    19021 cagaacgcga ttgcgctgat ggaagagatt gcggcgaatt tctcttacga gatgatccgc
    19081 ctgactgacc gcattctggg cttcacctgg aaccgatttt atcagggcat caacgtccat
    19141 aacgccgagc gcgttcgcca gctggcccac gacggtcatg agctggtgta tgtgccttgc
    19201 caccgcagtc acatggacta cctgctactt tcttacgtgc tgtaccacca ggggctggtg
    19261 ccgccgcata tcgccgccgg gatcaacctg aacttctggc cggccgggcc gattttccgc
    19321 cgtctggggg cgttctttat tcgccgtact tttaaaggca ataaacttta ttccaccgtt
    19381 ttccgtgagt atctcggcga actgttcagc cgtggttatt ccgtcgagta cttcgtggaa
    19441 ggcggtcgtt cccgcacggg gcgtttgctg gatccgaaaa ccggtacgct gtcgatgacc
    19501 attcaggcga tgctgcgtgg cggcacgcgt ccgattacgc tgattccgat ctatatcggt
    19561 tatgagcacg tcatggaagt gggtacttac gccaaagaac tgcgcggcgc gacgaaagag
    19621 aaagagagcc tgccgcagat gctgcgcggt ttaagcaagc tgcgtaatct cggtcagggt
    19681 tacgtcaact tcggtgaacc aatgccgttg atgacctacc ttaaccagca cgtaccagac
    19741 tggcgtgaat ctatcgatcc catcgaagcg gtgcgtccgg cctggttaac gccgacggtc
    19801 aataatattg ctgccgatct gatggtacgc attaacaacg caggcgcggc aaacgccatg
    19861 aacctgtgct gtactgcgct actggcatca cgtcagcgct cactcacccg cgagcagtta
    19921 accgagcaac tcaactgcta cctggatctg atgcgcaacg taccttactc cacggactct
    19981 accgttcctt cagccagcgc cagcgagctt atcgatcacg cgctgcaaat gaacaagttt
    20041 gaagtcgaga aagacactat cggcgacatc atcattctgc cacgcgagca agcggtgctg
    20101 atgacctact atcgcaacaa cattgcgcat atgttggtgc tgccttcgct gatggcggca
    20161 attgtcaccc agcatcgcca catctcccgc gacgtattga tggagcacgt caatgtgctt
    20221 tacccaatgc tgaaagcgga gctgttcctg cgctgggatc gcgacgagtt gccggacgtt
    20281 attgatgcgc tggcaaatga gatgcaacgt caggggctga ttaccctgca agatgatgag
    20341 ttgcatatca acccggcgca ttctcgcacc ctacagctgc tggccgcagg cgcgcgcgaa
    20401 acgctgcaac gttatgccat caccttctgg ttgttgagtg ccaacccgtc gatcaaccgc
    20461 ggtacgctgg agaaagagag ccgcaccgtt gcgcaacgtc tctccgtgct gcacggcatc
    20521 aacgcgccgg agttcttcga caaggcggtg ttcagttctc tggtgctgac gctgcgtgat
    20581 gaagggtata tcagcgatag cggcgatgcc gaaccagcag aaacgatgaa ggtttatcag
    20641 ttgctggcgg agttgattac atcagacgtg cgtttgacga ttgagagtgc gacgcagggc
    20701 gaagggtaat cagagagaat tgccggatgc ggcgaaaacg ccttatccgg cctaccatga
    20761 cctgcaaatt caataaattg cgattcaccg ggtaggcctg agaagcgcaa cgcatcaggc
    20821 aacccagaag aaagccggat gatcatccgg cttttttaca tcatcagaaa tgccagtaac
    20881 tcattgccag tcctaaaaac agtaccagac caacatagtt attattcata aatgctttaa
    20941 agcaggcttc acgctcgcgg ttggcaatca gtttttgttg ataaacaaac agcgcgccag
    21001 ccaccacaat tgaccagtaa tatccccagc ctaagccatt taactcaccg atgatcgcca
    21061 tcagtgccag tacgccaatc tgcaaaatac caataatcaa tttatcgtat tggccgaaca
    21121 ggattgccgt ggatttaatg ccaatcttca catcatcatc gcggtcaacc atcgcatact
    21181 gcgtgtcgta agccaccgcc cagagaatat tggcgaggaa cattaaccag caactcaatg
    21241 gcaccgactc actcacagcg gcaaaagcca ttggaatcga ccagccaaac gccgcgccca
    21301 gcaccacttg cgggagatgg gtataccgct tcataaacgg gtacacccac gccaatgcca
    21361 gagcggcaat cgacaacaga atggtcatcg tattcagcgt cagaaccagt aaaaacgaaa
    21421 tcagtaccag cactacaaac agcgcgcgcg cctctttctc tgttaccgcg ccgctgggaa
    21481 gtggtcggtt cgccgtgcgc ttaacatgac catcaaactt gcgatcagca taatcattca
    21541 ccacgcaacc agcagcgcgc atcagccaga cacccgcgac aaacaccgcc atgatccaga
    21601 gctgaggaac gcccggtgtc gccacccaca acgcccataa tgttggccag agcagcagta
    21661 acgcgccaat tggcttatcc gtacgcatta agcgatgaaa cgccagcagc ttattctgcg
    21721 tcagactcca ctccatattt ttttcctctt agtacaacgg tgacgccggt aaaaacagtt
    21781 ctgttagcaa cagcggttta ccgcttaatc gcaggcggga acgtcgcccc cacagtccgg
    21841 catcacggcc tatctcgata aaatcccggg ttagtgtcga tgatgtgaac agatagcgtc
    21901 ctaacggcgt tttacccaat ttttgtaacg ctagttccgg cccgcttaat gttgatacag
    21961 gaacgacggt acgaccggca agccacggtt caccatcggc acataacaaa atttcacgta
    22021 accagtaacg agactctttc ggcagcagcg gcagttcttc ggggatttca ttctgctcga
    22081 caaacccctc gcggatcatc gtcacactca ccgtttttcc ctgctgttca aaacgttttg
    22141 tcatggaatc ctccagcaac agccagtcga gcagttgcgg atccagggca gggatctctt
    22201 taaaatagcg cagcgcacgc agttgcgtta acgcggggtg tgacatgccg aactctccgt
    22261 tacataacgt aaaggcattg tatcgcagaa aagtgtttac gggagatgta aagcaaatta
    22321 agtgatctaa tgcgcaacaa aaacgcaaca atcgcatgaa gtggtaaaac gctgttgaac
    22381 cttacgcttt gccgatgccc gtgctggcga gggcaaccat aggggcatca ccgcccatga
    22441 tgctaggaac ggtgatgttt tcctttactt aaataccttt attctttata cgagtagctc
    22501 gccaactcgc agtaaaaatt gggaaaagcg catatttacc atgagatttg gagctgaaaa
    22561 gtgtttcctg agtaagatta ataggcaaca tcttatgtct ggatttatag atacacgtgg
    22621 aattccaatt ggtatctttt gtaggattca acatatcatg taagcgctgg gagcttacca
    22681 ccataacagg cccatcatcg acagaggaga acaggataaa tatattatcg tttcgtgtta
    22741 atttattagt gttaaaggtt cttaactttt cttcagtttc tataaatata ggtgaaacat
    22801 catcactacg aatccactct ttataaagat accaggcatt tgctttgatc atatcatccg
    22861 ttgatcgtaa ggcctgatcg ataagttgga taaaaaaatt gttattttta tcaaccagcc
    22921 ctttattcat attaaatctg ttaataaaat ggatcatctg ttgggtatcg aaattcacct
    22981 tactgaatcg atgttgacgc ataaaattat cgaacttgtc atcttcccca gaatgtatat
    23041 aactacgtat aattggatct tttatataag gacaatggct aaatgttttc ttcatattct
    23101 taataaagtt tttattaaca atattgagaa tttttaattg catcaccagt tttactgcca
    23161 gaacggattt caatatcatt gcattatctg gcaatgtatc aatcattttt ataatgttat
    23221 tgatataacc atcgagatct tcattcccaa atatatctga ataatcaata gttaattcca
    23281 attgttctgt aatgcaatct taaaaattcc ctgtcgacat tcgcacatga tcaaacataa
    23341 cagctgtcaa attcgattta ataaatgata tttctttggc agacagaaac tctgctctgg
    23401 tgaagtgcga agcgtaaaga taacaattat cgaagtttga atttctaatc tgacattcgc
    23461 gaaaattact atgaccaagt tgactctgcg agaagtcaac tgtatcaaga atattttggc
    23521 taaaatcaac acattcgcca ctcgttttga gaaaaatcac attgtgtaaa cgtcgacatt
    23581 taaatatgac attatcaact gttgccttag taaaattgca attctcaatt tctttacatt
    23641 cgaaagttac atgttgtaga tttgatgata aaaaagacac cttagaaata acgtcacctt
    23701 taaaatgaca atttgttaaa tcaaaatcag acaggtcgat atacccctgc gcatctttat
    23761 ccatcagaaa attacaagcg cattctattt tttcatttct gtagaatttt acacttatag
    23821 tatcattact aattgagact tcacgtaaca tatttattgc atgatttttt tcgttccgtt
    23881 gttttatatt taaaaatata tcactgatat aaataaagta atctctgttt aaacctgtca
    23941 ccccattgaa attacgtagt aaatcaagat ctactatatt atttccttgc accacatcat
    24001 gtttcatcaa agagttattg ccgccagaat ttgtgatatg acctatcata aaacctctgt
    24061 aatacagaaa taaaataacc tggaaaagta gcctgagcgt ccagacacta ccggcgaaag
    24121 gaaaagaatc tctgataatg cattgaaata atggacattc ttagtgaaga agaagtgaag
    24181 tactataaaa aatcgctcat atcccaggaa ggaagcataa aaaaaggtgc gccaggagac
    24241 gcaccagttg ttgcaaaatc agcactgtgg ggcgtaatta ccccttgcct tttacactgc
    24301 tgataaaggt ggaacgggca gatgtcgatc ccaggcgttc agcttcatca agcagtttta
    24361 acgccttatc aacatcacct ttcgcgacag cgtttttgat cgcggtatta aaataacttt
    24421 ccgtgtcgtt gagcatcggc tcgcttttct tcaccggcac cggagcaggt gcagccacag
    24481 ctggtgccgc cgtgttgcct accgtaaccg gagctggagc ggaagaacca aatagtggtc
    24541 ctaccaacac gctggagctg gagttcgttt tgactttcag tttcagtaag ccatcgctgg
    24601 tatgacgagc aaccggatcg ggaatatccg ggatcgagtt accgacgcct ttggcatagg
    24661 ctttagccgg gtcgagcagt tgggtcgtct gctggagatc tttttccgtg gtaaagacca
    24721 gaacataaag tttttgctgc cccaacgccg gtgtcaggcg cataacgcct tccagccgct
    24781 ctgcactcat cacgcctagt tcctggtagg tgaaataact gctggggaag aaggctgatg
    24841 gggtcatgtt ctgatcaaga atcagcacgt tcggcgcaaa aacgctggtt tgtttgttca
    24901 cttcgctggt cagcgtcagg ggcagttcgc caatgtttgc cgggacgctg tacgcagcaa
    24961 ccggaccact gatgccggga acgttcagtt gttggccgcc ggtcgccagt tgggtggtct
    25021 gggttttaga ttgatcgacc ggtgtccagg tgagttgttg cagcgcggca gatggaatgg
    25081 ctggcgcgtc gctggtgttt tgcggtacgt agttaacatc ggcaaggcta attccaggcg
    25141 cgcttgccag taaccctgct gataaacaga gggcgatgag acttttattc attttcattg
    25201 ttttcacctc aaaatctgga gctcagcggt agccaggcaa tagcgcgcta aacccgataa
    25261 tcagaggggc tttcgcccct ttcagataat gacaacctgt ttttatgccg gatgcggcgt
    25321 aaacgcctta tccggcccag gttttgctat taccaccaga tttccatctg ggcaccgaag
    25381 gtccactcgt cgctgtcgcc acgaccgaag ctgccgccgt tgaaatcagc aggaacggct
    25441 ttgccgtagt tcgggttaac cttgctatcg ccgttgtagt cgtaacccca tttctcatcc
    25501 cacttggcgt aggttgcgaa gacacgaata gccgggcgtg accagatgct gtcgccagcc
    25561 tgccattgtt gtgcgagggt aattttgtac tgattgttct tgtcgccggt gcgctgggat
    25621 tcgacgttgt cgtagccgat ttccatcacg gtgctcatga ttggcgtcca cttgtacatc
    25681 gggcgaatac caacggtcca ccacttggtg ccgttgtcgt tatcccagtt gatatcctgg
    25741 tacataccca cgtacatcat gtcccagttg tcgcccatgg agatcgcacc gtggtcgagg
    25801 atacgcagca tgtgaccgtt gttgttgata ttgtaggcaa atttttcgtt atcaaacgcg
    25861 acgccagaac cctgcgacag acctttaccc tgcgaggtca tcgagtcagt agcgtactga
    25921 acaacaaact tgttaaagcc cttcaggaca ctctgagtat gttcagcagt gaataaccag
    25981 ccgtctttcg atgcgccatc aaccagacga tagttatcac gcaggttggc acgaccgtag
    26041 tcgacaccca gttctaatgt gccgcccggg ttgatttcca tctgcgctaa acgcacatcg
    26101 aaaacgtcgt tcgcggtttc gttggtatag tcataaatat tgttgctggc aaaagaggaa
    26161 gaaccaccag cttcagagga gcgggttgct gccagagaga gtttaccgaa gccaacatcg
    26221 atgttttcca gaccggcacc aggaccagaa atatcccagt agtagaagtc gatcatatga
    26281 acgtcatgac gttggtagaa gcgcttacct gcccagatgg tggaacctgg cagccattcg
    26341 atcaggtttt ttccctgcac gtttgcttca cggaaggccg gatcggtagc ttcccagtca
    26401 ttctgttgcg cgacggaata ggccacgtta gtgtcgaaat agaagctctt atcgccctct
    26461 ttccacactt cctgacccaa ttttaattca gcataagttt cacattcgtt gccaagacgg
    26521 tatttacttt gagcaccggt agtctggaaa cactgttgtt caccgccgct acctgtccag
    26581 ccaataccgg aacgtgcata gccgtggaaa tcaacagcca ttgcctgagc agacattacg
    26641 cccgctgcga cggcaaccgc cagaggaagt ttgcgcagag taatcatcat tctatctcct
    26701 gagtcattgc ttttcttttt tcacatcacc tgtgacaggc tttgtgtgtt ttgtggggtg
    26761 cttaaacgcc cggctcctta tgcagtcgac gacatgcagt gccatcctca cggaacagat
    26821 ggcaacgctc tggcggcagg ccgatagcga atgtggcacc ttcttctacc aacaccacgt
    26881 cgttctggcg gtacaccagg ttttgacgaa tggaagggat ctggatatag atttgggttt
    26941 cgttgccgag ttgctcgacg acctgaactt caccctcaag gatgacgtca gcgatatcac
    27001 tcggcagtag atgttccggg cgaataccca gcgacatatt ggctccaacc tggacatcac
    27061 ggctttcaac aggcagccag acttgttgac gatttggcat cggcagctcc acctgcactt
    27121 gatcgattgc ggtggcggtc acttttaccg gcaggaagtt catctttggc gaaccgataa
    27181 atccggcgac aaaacggtct gccggatagt ggtacagttc cagcggtttc ccaacctgtg
    27241 ccacgcgacc ggcgtccagc accacgattt tgtcggccag cgtcatcgct tcgacctgat
    27301 cgtgggtgac gtaaatcatt gtgcggccca ggcgtttatg cagacgggag atttcgatac
    27361 gcatttgcac acgcagtgca gcatcgaggt tggagagcgg ttcatcgagc aaaaatacgc
    27421 ttggctcggc caccagcgta cggccaatcg ccacgcgctg acgctgacca ccggagagcg
    27481 ctttcggttt gcgatccagc aaatgcgcca gttgcagcac ttccgccacc tggttgacgc
    27541 gttggttaat cacctctttt tttgcgccag ccagtttcag gccaaatgac atgttttctg
    27601 ctactgacag gtggggatag agcgcgtaag actgaaacac cataccaacg ccgcgttctg
    27661 ctggcggagt gtcattcatc cgtttctcac cgatgaacag gtcgccgctg gtgatcgtct
    27721 caagcccggc aatcatgcgc agtaaagtcg atttaccgca gccagacggt ccgacaaaca
    27781 ccacgaattc accttcatgg atatcgagat tgatatcttt cgataccacg acctcgcccc
    27841 aggctttcgt tacattttgc agctgtacgc tcgccatgcc cttctctctt tgtaacaacc
    27901 tgtcatcgac agcaacattc atgatgggct gactatgcgt catcaggaga tggcttaaat
    27961 cctccacccc ctggcttttt tatgggggag gaggcgggag gatgagaacg cggcttctgt
    28021 gaactaaacc gaggtcatgt aaggaatttc gtgatgttgc ttgcaaaaat cgtggcgatt
    28081 ttatgtgcgc atccccacat taccgccaat tctgtaacag agatcacaca aagcaacggt
    28141 ggggcgtagg ggcaaggagg atggaaagag gttgccgtat aaagaaacta gagtccgttt
    28201 aggtgttttc acgagcactt caccaacaag gaccatagat tatgaaaata aaaacaggtg
    28261 cacgcatcct cgcattatcc gcattaacga cgatgatgtt ttccgcctcg gctctcgcca
    28321 aaatcgaaga aggtaaactg gtaatctgga ttaacggcga taaaggctat aacggtctcg
    28381 ctgaagtcgg taagaaattc gagaaagata ccggaattaa agtcaccgtt gagcatccgg
    28441 ataaactgga agagaaattc cctcaggttg cggcaactgg cgatggccct gacattatct
    28501 tctgggcgca cgaccgcttt ggtggctacg ctcaatctgg cctgttggct gaaatcaccc
    28561 cggacaaagc gttccaggac aagctgtatc cgtttacatg ggatgccgta cgttacaacg
    28621 gcaagctgat tgcttaccca atcgctgttg aagcgttatc gctgatttat aacaaagacc
    28681 tgctgccgaa cccgccaaaa acctgggaag agatcccggc gctggataaa gaactgaaag
    28741 cgaaaggtaa gagcgcgctg atgttcaacc tgcaagaacc gtacttcacc tggccgctga
    28801 ttgctgctga cgggggttat gcgttcaagt atgaaaacgg caagtacgac attaaagacg
    28861 tgggcgtgga taacgctggc gcgaaagcgg gtctgacctt cctggttgac ctgattaaaa
    28921 acaaacacat gaatgcagac accgattact ccatcgcaga agctgccttt aataaaggcg
    28981 aaacagcgat gaccatcaac ggcccgtggg catggtccaa catcgacacc agcaaagtga
    29041 attatggtgt aacggtactg ccgaccttca agggtcaacc atctaaaccg ttcgttggcg
    29101 tgctgagcac cggtattaac gccgccagtc cgaacaaaga gctggcgaaa gagttcctcg
    29161 aaaactatct gctgactgat gaaggtctgg aagcggttaa taaagacaaa ccgctgggtg
    29221 ccgtagcgct gaagtcttac gaggaagagt tggcgaaaga tccacgtatt gccgccacca
    29281 tggaaaacgc ccagaaaggt gaaatcatgc cgaacatccc gcagatgtcc gctttctggt
    29341 atgccgtacg tactgcggtg atcaacgccg ccagcggtcg tcagactgtc gatgaagccc
    29401 tgaaagacgc gcagactcgt atcaccaagt aatgctgtga aatgccggat gcggcgtgaa
    29461 cgccttgtcc ggcctacaaa actgaaacgc atgtaggcct gataagacgc atcagtgtcg
    29521 catcaggcag ttgttgtcgg ataaggcgtg aaagccttat ccgtcctgga atgaggaaga
    29581 accccatgga tgtcattaaa aagaaacatt ggtggcaaag cgacgcgctg aaatggtcag
    29641 tgctaggtct gctcggcctg ctggtgggtt accttgttgt tttaatgtac gcacaagggg
    29701 aatacctgtt cgccattacc acgctgatat tgagttcagc ggggctgtat attttcgcca
    29761 atcgtaaagc ctacgtctgg cgctatgttt acccgggaat ggctggaatg ggattattcg
    29821 tcctcttccc tctggtctgc accatcgcca ttgccttcac caactacagc agcactaacc
    29881 agctgacttt tgaacgtgcg caggaagtgt tgttagatcg ctcctggcaa gcaggcaaaa
    29941 cctataactt tggtctttac ccggcgggcg atgagtggca actggcgctc agcgacggcg
    30001 aaaccggcaa aaattacctc tccgacgcgt ttaaatttgg cggcgagcaa aaactgcaac
    30061 tgaaagaaac gaccgcccag cccgaaggcg aacgcgcgaa tctgcgcgtg attacccaga
    30121 atcgtcaggc gctgagtgac attaccgcca ttctgccgga tggcaacaaa gtgatgatga
    30181 gctccctgcg ccagttttct ggcacgcagc cgctctacac actcgacggt gacggcacgt
    30241 tgacgaataa tcagagtggc gtgaaatatc gtccgaataa ccaaattggc ttttaccagt
    30301 ccattaccgc cgacggcaac tggggggatg aaaagctaag ccccggttac accgtgacca
    30361 ccggctggaa aaactttacc cgcgtcttta ccgacgaagg cattcagaaa ccgttcctcg
    30421 ccattttcgt ctggaccgtg gtgttctcgc tgatcactgt ctttttaacg gtggcggtcg
    30481 gcatggttct ggcatgtctg gtgcagtggg aagcgttgcg cggcaaagcg gtctatcgcg
    30541 tcctgctgat tctgccctac gcggtgccat cgttcatttc aatcttgatt ttcaaaggat
    30601 tgtttaacca gagcttcggt gaaatcaaca tgatgttgag cgcgctgttt ggcgtgaagc
    30661 ccgcctggtt cagcgatccg accaccgctc gcacgatgct gattatcgtc aatacctggc
    30721 tgggttatcc gtacatgatg atcctctgca tgggcttgct gaaagcgatt ccggacgatt
    30781 tgtatgaagc ctcagcaatg gatggcgcag gtccgttcca gaacttcttt aagattacgc
    30841 tgccgctgct gattaaaccg ctgacgccgc tgatgatcgc cagcttcgcc tttaacttta
    30901 acaacttcgt gctgattcag ctgttaacca acggcggccc ggatcgtctt ggcacgacca
    30961 cgccagccgg ttataccgac ctgcttgtta actacaccta ccgtatcgct tttgaaggcg
    31021 gcgggggtca ggacttcggt ctggcggcag caattgccac gctgatcttc ctgctggtgg
    31081 gtgcgctggc gatagtgaac ctgaaagcca cgcgaatgaa gtttgattaa gggagataac
    31141 aaaaatggca atggtccaac cgaaatcgca aaaagcacgt ttgtttatta ctcacctgct
    31201 actgctactt tttatcgcgg cgattatgtt cccgctgctg atggtcgtcg ctatctcgct
    31261 gcgtcaggga aacttcgcga ccggtagcct gatcccggag caaatctcct gggatcactg
    31321 gaaactggcg ttaggtttta gcgttgaaca ggctgatggt cgcattacgc caccgccatt
    31381 cccggtactg ctgtggctgt ggaactcggt aaaggtcgct gggatttccg cgattggcat
    31441 tgtggcgctc tccaccacct gcgcctacgc tttcgcccgt atgcgctttc caggcaaagc
    31501 gacgctgctg aaaggaatgc tgattttcca gatgttcccg gcagtacttt cactggtcgc
    31561 gttgtatgcg ttgtttgatc gtctgggtga gtacattcca ttcattggcc tgaatactca
    31621 cggcggcgta attttcgcgt atctgggtgg gattgcgctg catgtctgga ccatcaaagg
    31681 ctatttcgaa accatcgaca gttcgctgga agaagcggct gcgctggatg gtgcgacacc
    31741 gtggcaggcc ttccgccttg tcctgttgcc gctgtcagta ccgattctgg cggtggtatt
    31801 catcctgtcg tttatcgctg ccattactga agttccggtc gcgtcgctgt tactgcgtga
    31861 cgtaaacagc tacaccctgg ccgtggggat gcagcaatac ctcaacccgc aaaactacct
    31921 gtggggtgac tttgccgccg ctgccgtgat gtctgcatta ccgatcacca tcgtcttctt
    31981 gctggctcaa cgctggctgg tcaacggcct gacggcaggt ggtgtgaaag gttaaagatg
    32041 ttgttctgcc aatgttatgc cgctgcaccc tcaacttacg ttatcccaac ttgtgactat
    32101 tattcggcgc tccacggagc gccttttttt c
//
LOCUS       DAJTQY010000040        32015 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.040, whole genome shotgun sequence.
ACCESSION   DAJTQY010000040 DAJTQY010000000
VERSION     DAJTQY010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32015
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.040"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            1..828
                     /gene="espL1"
                     /locus_tag="OO349_001929"
                     /pseudo
     CDS             1..828
                     /gene="espL1"
                     /locus_tag="OO349_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
     gene            1000..1107
                     /locus_tag="OO349_001930"
     CDS             1000..1107
                     /locus_tag="OO349_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5867016.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(1160..1918)
                     /locus_tag="OO349_001931"
     CDS             complement(1160..1918)
                     /locus_tag="OO349_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HCR5867017.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            2205..3134
                     /gene="pfkB"
                     /locus_tag="OO349_001932"
     CDS             2205..3134
                     /gene="pfkB"
                     /locus_tag="OO349_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HCR5867018.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            3235..3525
                     /gene="ghoS"
                     /locus_tag="OO349_001933"
     CDS             3235..3525
                     /gene="ghoS"
                     /locus_tag="OO349_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HCR5867019.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            3631..4491
                     /locus_tag="OO349_001934"
     CDS             3631..4491
                     /locus_tag="OO349_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HCR5867020.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACDWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(4532..5068)
                     /locus_tag="OO349_001935"
     CDS             complement(4532..5068)
                     /locus_tag="OO349_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HCR5867021.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFAHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            5215..5883
                     /gene="hxpB"
                     /locus_tag="OO349_001936"
     CDS             5215..5883
                     /gene="hxpB"
                     /locus_tag="OO349_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HCR5867022.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            6046..6636
                     /locus_tag="OO349_001937"
     CDS             6046..6636
                     /locus_tag="OO349_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR5867023.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            6769..8159
                     /gene="tcyP"
                     /locus_tag="OO349_001938"
                     /pseudo
     CDS             6769..8159
                     /gene="tcyP"
                     /locus_tag="OO349_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
     gene            complement(8163..8979)
                     /locus_tag="OO349_001939"
                     /pseudo
     CDS             complement(8163..8979)
                     /locus_tag="OO349_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843061.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9256..9519)
                     /gene="cedA"
                     /locus_tag="OO349_001940"
     CDS             complement(9256..9519)
                     /gene="cedA"
                     /locus_tag="OO349_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HCR5867024.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            9702..11963
                     /gene="katE"
                     /locus_tag="OO349_001941"
     CDS             9702..11963
                     /gene="katE"
                     /locus_tag="OO349_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HCR5867025.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKAMK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSTDGSFMDELLTLMAEHR
                     VWSRIPKIDKIPA"
     gene            complement(12010..12768)
                     /gene="chbG"
                     /locus_tag="OO349_001942"
     CDS             complement(12010..12768)
                     /gene="chbG"
                     /locus_tag="OO349_001942"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HCR5867026.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPVNLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDPSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(12781..14133)
                     /gene="celF"
                     /locus_tag="OO349_001943"
                     /gene_synonym="chbF"
     CDS             complement(12781..14133)
                     /gene="celF"
                     /locus_tag="OO349_001943"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCR5867027.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDCREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIHDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKVSGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGHDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(14238..15080)
                     /gene="chbR"
                     /locus_tag="OO349_001944"
                     /pseudo
     CDS             complement(14238..15080)
                     /gene="chbR"
                     /locus_tag="OO349_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
     gene            complement(15088..15438)
                     /gene="chbA"
                     /locus_tag="OO349_001945"
     CDS             complement(15088..15438)
                     /gene="chbA"
                     /locus_tag="OO349_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HCR5867028.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(15497..16846)
                     /gene="chbC"
                     /locus_tag="OO349_001946"
     CDS             complement(15497..16846)
                     /gene="chbC"
                     /locus_tag="OO349_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HCR5867029.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGSFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYAVGANWLGGANIISGIIIGLVVA
                     EMFTFIVRRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDIANP"
     gene            complement(16931..17251)
                     /gene="chbB"
                     /locus_tag="OO349_001947"
     CDS             complement(16931..17251)
                     /gene="chbB"
                     /locus_tag="OO349_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HCR5867030.1"
                     /translation="MEKKHIYLFCSAGMSISLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(17550..17888)
                     /gene="osmE"
                     /locus_tag="OO349_001948"
     CDS             complement(17550..17888)
                     /gene="osmE"
                     /locus_tag="OO349_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HCR5867031.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            18090..18917
                     /gene="nadE"
                     /locus_tag="OO349_001949"
     CDS             18090..18917
                     /gene="nadE"
                     /locus_tag="OO349_001949"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HCR5867032.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETRNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLTALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNLPEQVARTIENWYLKTEHKRRPPINVFDDFWKK"
     gene            19147..20034
                     /gene="cho"
                     /locus_tag="OO349_001950"
     CDS             19147..20034
                     /gene="cho"
                     /locus_tag="OO349_001950"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HCR5867033.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLHSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(19994..20569)
                     /gene="ves"
                     /locus_tag="OO349_001951"
     CDS             complement(19994..20569)
                     /gene="ves"
                     /locus_tag="OO349_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HCR5867034.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFTADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(20772..21257)
                     /gene="spy"
                     /locus_tag="OO349_001952"
     CDS             complement(20772..21257)
                     /gene="spy"
                     /locus_tag="OO349_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HCR5867035.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIHEIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(21587..22555)
                     /gene="astE"
                     /locus_tag="OO349_001953"
     CDS             complement(21587..22555)
                     /gene="astE"
                     /locus_tag="OO349_001953"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HCR5867036.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSVRHFGALVCTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(22548..23881)
                     /gene="astB"
                     /locus_tag="OO349_001954"
                     /pseudo
     CDS             complement(22548..23881)
                     /gene="astB"
                     /locus_tag="OO349_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
     gene            complement(23878..25356)
                     /gene="astD"
                     /locus_tag="OO349_001955"
     CDS             complement(23878..25356)
                     /gene="astD"
                     /locus_tag="OO349_001955"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HCR5867037.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVEQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLESNKAELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTVGQR
                     CTCARRLLLKSGAQGDAFLASLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQAGTSLLTPGIIEMTGVGGVPDEEVFGPLLRVWRYDTFDEA
                     IRMANNTRFGLFCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(25353..26387)
                     /gene="astA"
                     /locus_tag="OO349_001956"
     CDS             complement(25353..26387)
                     /gene="astA"
                     /locus_tag="OO349_001956"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HCR5867038.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(26384..27604)
                     /gene="astC"
                     /locus_tag="OO349_001957"
                     /pseudo
     CDS             complement(26384..27604)
                     /gene="astC"
                     /locus_tag="OO349_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
     gene            28050..28856
                     /gene="xthA"
                     /locus_tag="OO349_001958"
     CDS             28050..28856
                     /gene="xthA"
                     /locus_tag="OO349_001958"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HCR5867039.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGVVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            29023..29731
                     /locus_tag="OO349_001959"
                     /pseudo
     CDS             29023..29731
                     /locus_tag="OO349_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707363.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
     gene            29736..30413
                     /gene="ydjY"
                     /locus_tag="OO349_001960"
     CDS             29736..30413
                     /gene="ydjY"
                     /locus_tag="OO349_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
                     /protein_id="HCR5867040.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYAPPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKHGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            30428..31135
                     /locus_tag="OO349_001961"
     CDS             30428..31135
                     /locus_tag="OO349_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR5867041.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTELFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            31135..31683
                     /locus_tag="OO349_001962"
     CDS             31135..31683
                     /locus_tag="OO349_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HCR5867042.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            31693..>32015
                     /locus_tag="OO349_001963"
     CDS             31693..>32015
                     /locus_tag="OO349_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR5867043.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTL"
BASE COUNT         8222 a         8210 c         7581 g         8002 t
ORIGIN      
        1 catcggcaat gttgttaata atggctacag gccagactca acagcttatt acattattca
       61 aacagttgcc catactccct gaaaaagaaa tcattgaaat aataactgcg cagaatagcg
      121 ttggtacacc tgctttattt ttggctatga tgaacggaca tactgacaac gtgaaaatat
      181 ttatgcaaga aattcagtca ctggtagata atcacatcat tcatgaagat aatctggtta
      241 aattactgca aactaaaagt gctaacgaaa cacctggact ttatatctcc atgttgtatg
      301 gattcgatga aataatcgat atctttctga atacattaac cactccaata gcactaagag
      361 cttttaaaca aaaaactggt gatgagtatt ttagccatga aaatacatga tggtgagcca
      421 ggattatacg ccgcaatgga aaataatcac cctttgtgtg tcacacggtt cctctctaaa
      481 attaatggca tcgcctttaa atacaagttg agcaaagcta acatcatgga tttattaaaa
      541 ggagctacag cacagggaac ccctgcatta tacatcgcca tgagcaaggg taatgaagac
      601 gtcgtgttat cttatatatc gacgctgggt gcttttgcaa aaaaacattc ttttagtcaa
      661 catcagttat ttacactatt ggccgctaaa aatcatgaca acatgtcagc tgttcatata
      721 gccattcatc ataagcatta taaaactgta gaaacatatt atgctgctat aaatgtcatc
      781 agccaaagcc tgagttttag tgctgatgaa ataaagacgt atttataacc agctatttac
      841 gatatatatc gatacataat tttatttttt tcataaaacg cagaaacagt gaccatgtgc
      901 acaacgccac ccgacggctg taataacaaa aaatcccttg atgcctgtcc cttttgttac
      961 actccgttat cacgcacaag agatatgcag gacactggta tgccgactaa acgctttgat
     1021 aaaaaacact ggaagatggt ggtggtgcta ctggcaatct gtggcgctat gttgttgcta
     1081 cgttgggcag caatgatttg gggctgagca gtgacaaacg aagacagcca gaccgcataa
     1141 cacggtccgg cagataattt tacatgctgt agcccagcga taaggttgtc cgacggtcgg
     1201 tatgttccgg tgcagactct ggaggttcag agttccatgt gacgttgtaa gccaccttca
     1261 gtccaaaatg ttcattaata gcaacattta atgcgctttc ggagttcagc gttgtgtctt
     1321 ccgcgccaaa gaccgaaaca ccctgcgtaa atttagcgtt gtcagtcaac tgccatgcat
     1381 aagccccgga ggcatagccc agcggctggg tttcactggc attgtcggta tatttgtcgt
     1441 aacgcacacc tggaccgaat tcaaagcgga aactgtgtac cgggccattc aaaaactgac
     1501 gaccataacc cgcggtcaac acatcgcgct ctcgatagcc gttataacgg tctgtcagcc
     1561 agcttgcctg accaaataaa tagtcataat cagttaaatt aaagcggcta cgcccgcccg
     1621 ccgcatattt ttctgaagaa cgctcatcat tagaagaagt attactggcg ttcccccaca
     1681 gcgaccaggc agtggtttgt ccataccagg tcatggtggt gtcagccgta agtgaggagc
     1741 ttttcgtatt gcctgattgt gcaagatatc ctgcgttcag attaccttcg aaaggttttt
     1801 tagcgctggc aggatcgtcc atgacagtaa aaacggaatc atcggcagct gcattcagtg
     1861 acgcaaacat gccccccgcc aacataacga tggcaggaac tgtcttcaaa agcttcattt
     1921 atcaagagtc cgtacaacaa aaaaagagac catcgcggtc ccggaaactt tcttaaggat
     1981 caaagattag cgtccctgga aaggtaacga attataaaaa ggcgcgaata acttagcaat
     2041 gtattcttat ttcatttttt gaataagcat gtggcgaaaa cagattttta tttatatata
     2101 tttatctgca aaattttaaa taaagctcta ataaatcata ttgttaattt cttcactttc
     2161 cgctgattcg gtgccagact gaaatcagcc tataggagga aatgatggta cgtatctata
     2221 cgttgacact tgcgccctct ctcgatagcg caacaattac cccgcaaatt tatcccgaag
     2281 gaaaactgcg ctgtaccgca ccggtgttcg aacccggggg cggcggcatc aacgtcgccc
     2341 gcgccattgc ccatcttgga ggcagtgcca cagcgatctt cccggcgggt ggcgcgaccg
     2401 gcgaacacct ggtttcactg ttggcggatg aaaatgtccc cgtcgctact gtagaagcca
     2461 aagactggac ccggcagaat ttacacgtac atgtggaagc aagcggtgaa cagtatcgtt
     2521 ttgttatgcc aggcgcggca ttaaatgaag atgagtttcg ccagcttgaa gagcaagttc
     2581 tggaaattga atccggggcc atcctggtca taagcggaag cctgccgcca ggtgtgaagc
     2641 tggaaaaatt aacccaactg atttccgctg cgcaaaacca agggatccgc tgcatcatcg
     2701 acagttctgg cgaagcgtta agtgcagcac tggcgattgg taacatcgag ttggttaagc
     2761 ctaaccaaaa agaactcagt gcgctggtga atcgcgaact cacccagcca gacgatgtcc
     2821 gcaaagccgc gcaggaaatc gttaatagcg gcaaggccaa acgggttgtc gtttcccttg
     2881 gtccacaagg agcgctgggt gtcgatagtg aaaactgcat tcaggtggtg ccaccaccgg
     2941 tgaaaagcca gagtaccgtt ggcgctggtg acagcatggt cggcgcgatg acactgaaac
     3001 tggcagaaaa tgcctctctt gaagagatgg tccgttttgg cgtagctgcg gggagtgcag
     3061 ccacactcaa tcagggaaca cgtctgtgct cccatgacga tacgcaaaaa atttacgctt
     3121 acctttcccg ctaacaaaaa cattccccca gcattggggg aatcaccacc aacctgtcgg
     3181 caacgcgttt ctccgactat gctcaaaagt catgtgataa caaaggggtg aactatggcc
     3241 agtggcgatc ttgtccgtta tgtcataacc gtcatgttgc atgaggatac attgactgaa
     3301 attaacgagt tgaataatta cctgactcgc gacggttttt tgctcaccat gacggatgat
     3361 gagggaaata tccatgagct ggggactaac acttttggac ttatcagtac ccaaagtgaa
     3421 gaagaaatta gagaactggt ttcggggctt acccaaagtg caaccggcaa agatcctgaa
     3481 atcaccatca cgacctggga ggaatggaat agcaacagaa aataaatggt ttttgggcaa
     3541 taatcagtct gtggtgtgcg ttagctcgtg tttttacacc gcattcttgc gctaacctta
     3601 tgatctggca gacaacatgg gagagacatc atgtggcagg caatcagtcg tcttttgagc
     3661 gagcagttag gtgaaggcga aatcgaactg cgtaatgaac tgcctggcgg agaagtccat
     3721 gccgcatggc atttgcgcta tgcaggacat gactttttcg tcaaatgtga tgaaagggaa
     3781 ctgcttcccg gttttaccgc cgaagccgac caactggagt tactgtcgcg tagtaaaacc
     3841 gtcaccgtgc ctaaggtttg ggcagtaggc gctgaccgtg actacagttt tctggtgatg
     3901 gattatctcc cacctcgtcc gctggatgcg catagcgcat ttattcttgg tcagcaaatt
     3961 gcgcgtttac atcaatggag tgaccaacca caatttggcc tcgattttga taactcgctc
     4021 tccacaactc cacagcccaa cacctggcaa cgtcgctggt caacgttttt tgctgaacaa
     4081 cggattggct ggcagttgga actggcagca gagaaaggga tcgctttcgg caatatcgac
     4141 gccatcgtcg agcatattca gcagcgtctg gcctcgcatc aaccgcagcc ttctctgttg
     4201 cacggcgatt tatggtccgg caactgtgcg ctgggtccgg atggcccgta cattttcgac
     4261 cctgcctgcg actggggtga ccgagagtgc gacctagcga tgttaccgct gcatactgaa
     4321 cagccgccac aaatctatga cggctatcag tcagtatccc cgctacctgc cgatttcctt
     4381 gaacgtcaac cggtttacca actctacacg ctgttaaatc gtgcaagatt atttggcggt
     4441 cagcatttgg ttattgctca gcagtcattg gatagattat tagcagcatg atatgggttg
     4501 aggataatgg ccgctccgtg cggccttttg attaaataaa cccaagcaga gaaaagaaca
     4561 cataacctgc agcaatacaa ataaccggca ggatatatag tgagaaaaac tgcaggaaaa
     4621 tagtatggtg cggaacaaca atacgggact caatttgttc acgcgttagc ccctcttccc
     4681 ctttggcttt ttccagaatg agttgatctt caacaccttc ccgcaagaag cgcgcctggc
     4741 gactcatccg ggcaccggaa tcctgcaacg ccagtccgac aaaaatcagg atgaaaatca
     4801 cccagaacat aacgttcaca ccaccattaa aattaggggt cggggagtta taccagaaca
     4861 gattcaaaaa aggcgtattc gcctgcatca tatcgatcat gacatgagca aagtcgagca
     4921 tgaccgcatt aatgccttcc tggttttcct gccgggtatt cataaatttc agcaaagaaa
     4981 tcaatgtaga gatcaatgca ggtataaaaa tcacccaacc caaaatcctt ttcaaaacag
     5041 caatgcgtcc agcttgttga tacgtcatga gttctccttg attaagacgc gtcgtttcac
     5101 ttagtttacc tgtagatatc tgttttcgcc cattctttaa aggcgatatg ataggcgctt
     5161 aatcataagc acggcttaat accttacaca taatgctcta aaggagaggt tgtaatgtca
     5221 accccgcgtc agattcttgc tgcaattttt gatatggatg gattacttat cgactcagaa
     5281 cctttatggg atcgagccga actggatgtg atggcaagcc tgggggtaga tatctcccgt
     5341 cgtaacgagc tgccggacac cttaggttta cgcatcgata tggtggtcga tctttggtac
     5401 gcccggcaac cgtggaatgg gccaagccgt caggaagtag tagaacgggt tattgcccgt
     5461 gccatttcac tggttgaaga gacacgtcca ttattaccag gcgtgcgcga agctgttgcg
     5521 ttatgcaaag aacaaggttt attggtggga ctggcctccg cgtcaccact acatatgctg
     5581 gaaaaagtgt tgaccatgtt tgacttacgc gacagtttcg atgccctcgc ctcggctgaa
     5641 aaactgcctt acagcaagcc gcatccgcaa gtatatctcg actgcgcagc aaaactgggc
     5701 gttgacccac tgacctgcgt ggcgctggaa gactcggtaa atggcatgat cgcttctaaa
     5761 gcagcccgca tgcgttccat cgtcgttcct gcgccagaag cgcaaaatga tccacgtttt
     5821 gtattagcaa acgtcaaact ctcatcgctg acagaactca ccgcaaaaga ccttctcggt
     5881 taatgaccag gggcagtgat cacgctgccc ctggttcttt atctgaattt gcattcaact
     5941 gacggattaa tcgtcaattt aagagaaaga gttacaccgt caccacttcc gtgcactgta
     6001 taaaaatcct atactgtacg tatcgacagt ttagtgagtt ttatcatgac ggcggaaggt
     6061 caccttctct tttctattgc ttgtgcggta tttgccaaaa atgccgagct gacgcccgtg
     6121 ctggcacagg gtgactggtg gcatattgtc ccttccgcaa tcctgacgtg tttgttaccg
     6181 gacatcgatc acccaaagtc gtttcttggg cagcgattaa aatggatatc aaaaccgatc
     6241 gcccgcgctt ttgggcatcg tggttttacc cacagtctgc tggcggtatt tgcgctgctg
     6301 gcaacctttt accttaaggt tccggaaggc tggttcattc cggctgatgc gctacaagga
     6361 atggtgctgg gttatttgag ccacatactt gccgatatgc tgacacccgc cggtgttccc
     6421 ctgctctggc catgccgctg gcgtttccgc ttgcctatcc tggttcccca aaagggcaac
     6481 caactggaac gttttatctg catggcatta tttgtctggt cggtatggat gccccattca
     6541 ttacccgaga acagcgctgt tcgttggtca tcgcaaatga tcaatacctt gcagatccag
     6601 tttcatcggc ttattaagca tcaggttgaa tactaaaaag gcaaaaatca cctttctgga
     6661 ataagcaatt ccatttgaat ataagagcca gctcacagtt ctgttaatct tgcgccaaca
     6721 ctatgactgc tacgcagtga tagaaataat aagatcagga gaacggggat gaactttcca
     6781 ttaattgcga acatcgtggt gttcgttgta ctgctgtttg cgctggctca gacccgccat
     6841 aaacagtgga gtctggcgaa aaaagtgctg gtgggtctgg tgatgggtgt ggtttttggc
     6901 cttgccctgc ataccattta tggttctgac agccaggtac ttaaagattc tgtacagtgg
     6961 tttaacatcg ttggtaacgg ctatgttcaa ctgctgcaaa tgatcgttat gccgttagtc
     7021 ttcgcctcta ttctgagcgc ggttgcccgt ctgcataacg catctcagtt aggcaaaatc
     7081 agttttctga ccatcggtac gcttttgttt accacgctga ttgcggcgct ggtcggtgtg
     7141 ctggtcacca acctgtttgg tttgacggct gaaggtctgg ttcagggtgg tgcagaaact
     7201 gcacgtctga acgccattga aagtaactat gttggtaaag tctctgatca gagcgttccg
     7261 cagctggtct tgtcctttat cccgaaaaac ccgtttgccg atcttaccgg agccaatccg
     7321 acgtcaatca tcagcgtggt aatttttgcc gcattcctcg gcgtagctgc attgaaactg
     7381 ctgaaggatg atgcgccgaa aggggaacgc gtcttaaccg ctatcgatac cctacaaagc
     7441 tgggtgatga aactggttcg ccttgtcatg cagttgaccc cttacggcgt tctggctcta
     7501 atgaccaaag tggttgcagg ttctaacttg caagacatca tcaaactggg aagtttcgtt
     7561 gtcgcgtcct acctcggtct gctgattatg tttgctgtgc atggcattct gctgggcatt
     7621 aatggcgtga gtccgctgaa gtacttccgt aaggtatggc ctgtgctgac gtttgccttt
     7681 accagccgtt ccagtgctgc gtctatccca ctgaatgtgg aagcacaaac gcgtcgtctg
     7741 ggcgttcctg aatccatcgc cagtttcgcc gcctctttcg gtgcaaccat tggtcagaac
     7801 ggctgcgccg gtttgtatcc ggcaatgctg cggtgatggt tgcgcctacg gttggcatta
     7861 acccgctgga cccgatgtgg attgcgacgc tggtcggtat tgttaccgtt agttccgcag
     7921 gcgttgccgg tgtcggtggt ggtgcaactt tcgccgcact gattgtgctg cctgcgatgg
     7981 gcctgccagt aaccctggtg gcgctgttaa tctccgttga accgcttatc gacatgggcc
     8041 gtacggcgct aaacgttagt ggctcgatga cagctggcac gctgaccagc cagtggctga
     8101 agcaaaccga taaagccatt ctggatagcg aagacgacgc cgaactggca caccgttaat
     8161 tcttatgctg gcaacggtcc gttttgtata ggggccgttg ctttactttt taaatgttcc
     8221 atcgcggcga tgcatactga ctcatattta aacagatcgt cagcgaaaaa aatgtccaac
     8281 ttatcaatac attcctggct caatgaatgt gcctgtagtg agaagcttaa aacctcatac
     8341 ccgagacgtt ttctaagtga actatctgga atactcataa tatcttcaag cacaccactt
     8401 cccattcccg gaagatttag tggattaaaa agaagattga tattaagcat tgatattatc
     8461 gaaggaaact ttaggagact ctcatttttt ttgaatcgca gcaaaaatgc tcccggcagg
     8521 atgtatataa tttttttcat agtatatagt ccttaaataa tgaataacca acctattttt
     8581 tatattgatc aaattcgtca ttttttcggg tcggaattca caggtaaaat ccatcccaac
     8641 aaaattctca ttaatatctc ttatccgaaa ctcactgcca taaagattat taattatcac
     8701 tctatattta acattgtcct gcagatacgt atcaaaacta agtgctggcg ctcctagctt
     8761 tgaggcatca tagttaatgt tagacatata ggcaatcata cgattgaagt tcgactctgc
     8821 tatttcatcg cagtcacatt gacccgttga tataaagaaa gtgagtgccg ccatggtaga
     8881 ctcaagcctt tctttaaaag agtcgaaagc caggctttta atatttaaat caccataatt
     8941 actctgtatt aagtttgtag aaaacatctc ccgcctcata ttgttaacaa aattattatc
     9001 ttatttaaat ctaagtcatt tacaatataa gtttaagagc gacgccacag gatgaactat
     9061 caaaaataac tcatcatgat tagcaaaact taaccatttt aaaataaata aataattaaa
     9121 gaaaaaagat cacttattta tagcaatagc tcgtcaaagg cagctttttg ttacaggtgg
     9181 tttgaatgaa tgtagcaacg aaatgcagaa tttcaggtcc tgtaactccc ggcaaaaccg
     9241 ggaggtatgt aatccttact cagtcacttc cccttcctgg cggatctgat ttgcccaacg
     9301 ttgggcagat tcaggcacag taaacgccgg tgagcgcaga aatgactctc ccatcagtac
     9361 aaacgcaaca tatttgccac gcagcatcca gacatcacga aacgaatcca tctttatcgc
     9421 atgttctggc ggcgcgggtt ccgtgcgtgg gacatagcta ataatctggc ggttttgctg
     9481 gcggagcggt ttcttcatta ctggcttcac taaacgcata ttaaaaatta gaaaaactgt
     9541 agtttagccg atttagcccc tgtacgtccc gctttgcgtg tatttcataa caccgtttcc
     9601 agaatagtct ccgaagcggg atctggctgg tggtctatag ttagagagtt ttttgaccaa
     9661 aacagcggcc ctttcagtaa taaattaagg agacgagttc aatgtcgcaa cataacgaaa
     9721 agaacccaca tcagcaccag tcaccactac acgattccag cgaagcgaaa ccggggatgg
     9781 actcactggc acctgaggac ggatctcatc gtccagcggc tgaaccaaca ccgccaggtg
     9841 cacaacctac cgcccctggg agcctgaaag cccctgatac acgtaacgaa aaacttaatt
     9901 ctctggaaga cgtacgcaaa ggcagtgaaa attatgcgct gaccactaat cagggcgtgc
     9961 gcatcgccga tgatcaaaac tcactgcgtg ccggtaaccg tggtccaacg ctgctggaag
    10021 attttattct gcgcgagaaa atcacccact ttgaccatga gcgcatcccg gaacgtattg
    10081 ttcatgcacg cggctcagca gctcacggtt atttccagcc atataaaagc ttaagcgata
    10141 tcaccaaagc ggatttcctc tcagatccga acaaaatcac cccagtattt gttaggttct
    10201 ctaccgttca gggtggagct ggctctgccg ataccgtgcg tgatatccgt ggttttgcca
    10261 ccaagttcta tactgaagag ggtatttttg acctcgttgg caataacacg ccaatcttct
    10321 ttatccagga tgcgcataaa ttccccgatt ttgttcatgc ggtaaaacca gaaccgcact
    10381 gggcaattcc acaagggcaa agtgcccacg atactttctg ggattatgtt tctctgcaac
    10441 ctgaaactct gcacaacgtg atgtgggcga tgtcggatcg cggcatcccc cgcagttacc
    10501 gcaccatgga aggcttcggt attcacacct tccgcctgat taatgccgaa gggaaggcaa
    10561 cgtttgtacg tttccactgg aaaccactgg caggtaaagc ctcactcgtt tgggatgaag
    10621 cacaaaaact caccggacgt gacccggact tccaccgccg cgagttgtgg gaagccattg
    10681 aagcaggcga ttttccggaa tacgaactgg gcttccagtt gattcctgaa gaagatgaat
    10741 tcaagttcga cttcgatctt ctcgatccaa ccaaactgat cccggaagaa ctggtgcccg
    10801 ttcagcgtgt cggcaaaatg gtgctcaatc gcaatccgga taacttcttt gccgaaaacg
    10861 aacaagcagc attccatcca ggtcatattg ttcctggtct ggacttcacc aacgatcccc
    10921 tgttgcaggg acgtttgttc tcctataccg atacacaaat cagtcgtctt ggtgggccga
    10981 atttccatga gattccgatt aaccgcccga cctgccctta ccataatttc cagcgtgacg
    11041 gcatgcatcg tatggggatc gacactaacc cggcgaatta tgaaccgaac tcgatcaacg
    11101 ataactggcc gcgcgaaaca ccaccggggc cgaaacgcgg cggttttgaa tcataccaag
    11161 agcgcgtgga aggcaataaa gttcgcgagc gcagcccatc gtttggcgaa tattattccc
    11221 atccgcgtct gttctggcta agtcagacgc cattcgagca gcgccatatt gtcgatggtt
    11281 tcagttttga gttaagcaaa gtcgttcgtc cgtatattcg tgagcgcgtt gttgaccagc
    11341 tggcgcatat tgatctcact ctggcccagg cggtggcgaa aaatctcggt atcgaactga
    11401 ctgacgacca gctgaatatc accccacctc cggacgtcaa cggtctgaaa aaggatccat
    11461 ccttaagttt gtacgccatt cctgacggtg atgtgaaagg tcgcgtggta gcgattttac
    11521 ttaatgatga agtgagatcg gcagaccttc tggccattct caaggccatg aaggccaaag
    11581 gcgttcatgc caaactgctc tactcccgaa tgggtgaagt gactgcggat gacggtacgg
    11641 tgttgcctat agccgctacc tttgccggtg caccttcgct gacggtcgat gcggtcattg
    11701 tcccttgcgg caatatcgcg gatatcgctg acaacggcga tgccaactac tatctgatgg
    11761 aagcctacaa acaccttaaa ccgattgcgc tggcgggaga cgcgcgcaag tttaaagcaa
    11821 caatcaaggt agctgaccag ggtgaagaag ggattgtgga agctgacagc accgacggta
    11881 gttttatgga tgaactgtta acgctgatgg cagaacaccg cgtgtggtca cgcattccta
    11941 agattgacaa aattccggcg taaatatgga taaggtcacg tcccctgggg agtgacctta
    12001 ccaccttact tacacatcaa gataactacc caaccgataa ccacgctcag caattgcata
    12061 tttcaacgac gctgatgtca gcacttccag ctccgttaac cgtgggaaac agtaagcact
    12121 ctgacggata gtattatcga taaacgccgg atgacacatc acctccagcg aaggatcacc
    12181 ccgatggctt gcgtcatcca gcacttgcag gaacagtgcc tcgctaatct cttcaccata
    12241 aaacgcgcta ctgaatccct gcgaactgcg cagattaacc ggtaaatcac cagcgttaga
    12301 tagcggctga cggtcgatgc gcaacgcaat cccctcttca gccgcaaacc tggcaacaat
    12361 cgggaaaatc tgcgggaaca tatgcacatg atgatggcta tcaagatgcg taggtttgcg
    12421 tccaaatagc tcaatgaaac gcaaatactg actggcaagc tcctgagtaa tttcttccag
    12481 cggtaaagca tcttcttctg ccaactgcca gatccatttt cccagcacac catcgcgggt
    12541 taaccccggc atagctgtca gtggcttgcc catagtaagg acaaagtgca tccctatggc
    12601 cagactcggt tcatcacgac tcaactgcac cgcatggtca atagcctgcc cattcaccag
    12661 cgccgtcgtc gatgtgacaa tcccattgcg acaggcctcg ataatgccgt agttctgacc
    12721 tttgcttaag ccaaaatcat cggcattaac aatcagtaag cgttccataa tcagcctcgg
    12781 ttaatgtgct tttttaagct ctgcgatgca gtcggcaaaa tttggcagcc atttctcgtg
    12841 cgccagaatc atctcgcgtg ccagcagctc agcatcgcga tcggaatgca ccaacggact
    12901 aaggtttagc gccagtaaca catcgttaaa ttctccgcta agtgcggcgt tgctggcagc
    12961 aatctcgaag cctttaatgg tgtgaatcag ccccatcact ttatcatcga aatgcgtaat
    13021 gcgcggatgt ggcgtcgcgc catcgtgccc cagcttacag gtcatttcta ccgcccagtc
    13081 tgccggaata ttatcaatat gcccatgatg cgggatatta acgtaatgtt cagcttgctt
    13141 gtcgttgtag atagcgttga tcacttcgca cgctgcatca gagtaataag ccccaccgcg
    13201 ctgttccagt tctttcggct taactttcaa ctccgggttt ttatacagct caaaaagttg
    13261 tttctcgact ttctgtacta cctgcgctcg tgcgccgcct ttgtagtatt cgcccatttc
    13321 aatagccagc atctcttttt gcttgaagta ataaagcaaa taagaacacg gcaacagatt
    13381 cagagaacga attaagcctt cgctaaatgg cagatcgaaa atatttttaa cgccagatac
    13441 ttttaactgc ccggatgcca caccatcaag caattcggca aagcgcgact tgccatttac
    13501 cagcacatcc ttaatgaaca ccatatggtt gaggccaaac aaatcgatag ataaatcatc
    13561 gctgtctttc agcatcagaa catcgtgaat aaacatcttc atgccgatcg gaatattaca
    13621 cacgccgata aaacgtttaa atccggtatg acgataaacg gcttcagtaa ccattcccgc
    13681 cgggttagtg aagttaatca cccatgcatt cggacaaagt tcttcgacat cttttacgat
    13741 gtcaaaaatc accggaatgg tacgcagacc tttaaacaaa ccgcccgcgc cgttggtttc
    13801 ctgaccaaga taaccatgac ttaatggaat acgttcatcc agttcacgcg ccggtaattg
    13861 gccaacgcgc agttgggtag taacgaaatc agcatctttc aatgcttcgc ggcaatccag
    13921 cgttttataa agcttcatcg ggacgccagc gttatcaatc atccgttggc agagatcgaa
    13981 aataatatcc agtttagctt tgccaccctc gacatccacc agccataatt cgctgaccgg
    14041 caattcgtga taacgcttaa taaatccttc cagtaactcc ggggtatagc tgctcccgcc
    14101 accaatagtg acgactttta atttctggct cataatttct cccttcagta cagaatactg
    14161 atatctggca tatctgcccc cccggacata aataatccag caacaggaca gatatgtgaa
    14221 ttgtcaggta taacgactta ctgattaaat tcagtcaatt tcttacgata tctcttaggc
    14281 gtaaaggacg ttaatttctt aaacgtttta ataaacaaac tcgggctact ataaccggcc
    14341 tcaaacgcaa tatccgtcac ggaatagttg gtcatttcca gttgtttttt tgcaaaatta
    14401 atacggatct cattaataat ctgcattggt gttttgccat aatatcgttg agtcgctcgc
    14461 gtcaaatatt cctgtgattt ggctgacaac gtcaccatat tctccagcgc cgattcacta
    14521 aactgctctt tatcatgcat cttttctacc gtacttttca gccactacgg tacatcatca
    14581 atcacctgtt cttcgcggta atgacgtaaa cggttaatga cataaaaagt aaccatctca
    14641 acaaactctt ccagccctgt ttcgcggaaa tttaatgaag aaatcactgt ttccacatag
    14701 gtgagaaaag cgttattggt ccggtatacc tgcgaagcaa caaagcaata aggcaacaac
    14761 ggcaggtaat gctgctcaaa aaagcgttta ctgatcccaa cgttcaatat gcgcgtggca
    14821 ccaaactcat aaaaactttg atggtgcgaa cctaacggaa taaaaacaaa atcgccccgt
    14881 tccagtaaca cgcgcttacc gttaatctcc tggaaataac gcccggttaa tactagagta
    14941 aattcataat agtcgtgctg atgcagtccg ctgatactct cagttttgtt atagataaac
    15001 acatggaagt ttttgccatt aaacaactgc tgttctcggg cagtggcaat ttccggcgcg
    15061 ttaatcactg gctgcatcat cgactcctta tgccttcagt ttttcatgaa gctcaattaa
    15121 ttcagtaatc agttcacgcg caagcatgga cgtcattaaa tgatcctgag cgtggaccag
    15181 caccagactc actttcatct taccttcgcc cgcatcgcct tcaatcagtt tcgtctgtac
    15241 cagatgcgct tcattcaatg ccatccgtga ctgatccatc atggctttcg ctgcggcaaa
    15301 atcgccctgc ttcgcctgtt tcaatgcggc atacgccagg ctacgcgctt gtccagagtt
    15361 gatgataagc cccatcacca cttcttccag ctcttcagct tctgtttgcg tatcaggaat
    15421 attatcgaga tccatcatac atcgttcctc tctcttctta ccggcaagat tacccgtacc
    15481 ggcatcgatt aaaatttcag gggttagcga tatcttcttc gctctcttct ttatcaatcg
    15541 cattttgtgc tttgttagcc accacaacaa agggcagata aattaacgtt gcgatgccaa
    15601 ggttgaagag tgcgaccagc aatgcggcga cgctaccgtt ggtgttaaag aaggctccca
    15661 gaccggttgg catggtccac ggtgcaatat tggtcaccgg aggaatgatg cccatgtagt
    15721 acgctgcgag ggtgattgcc gccagaatcg gttgtaccag tacaaacggg ataaacatca
    15781 ccgggttcat gataattggc agaccaaaca gaatcggttc gttaatctgg aagatgccgg
    15841 acggcagcgc cagttttgcc acctgacgat aatcagcacg gcgagaggcg ataaagatag
    15901 ccaggatcag gcctaaagtc gcaccactgc ccccaaggaa aataaaggag tccagcatcg
    15961 gcttggccca gatatggaag gtcttaccgg ctgccagcgc cgcttcgacg gaaccatatt
    16021 gctgataggt cgcgatattt tccagcgccc acggcgtcat aatgccgttg tccagtgcgg
    16081 tcagcgccag cgcgccatga ataccgaaga accagagcag tggaacaaag atcacatagg
    16141 cccagcccac cacgctaccc aacgatgcca gtggggttga gatggtatcc ataatgatct
    16201 gatggaagtt ggtgccccag gtattcaacg cccaggcaat aatccccatc acggaaagaa
    16261 taataaagcc gggaattaat gccgagaaag aacgcgatac tgaagcaggt acgctgtcgg
    16321 gcagtttaat gacccaattg cggcggacaa taaaggtaaa catttctgcc accaccaggc
    16381 caataataat cccggagatg atattcgccc cacctaacca gtttgcgcca accgcatagg
    16441 cctcaccgac actatatggg gtgacggtca taaatgctgc aacggataac aacccagccg
    16501 ccagcgcatc gactttacgc tcttctgcca gcgccatgcc aataaagaac ggtgccatta
    16561 aagacattat tcctaatgtt ccgttatata cgttgccgcc aatacctttc agaccattaa
    16621 gtgtttcaat ggttgaggcg tcgaggcgaa tacctaagga ataaaaaaac gacccctccc
    16681 caaagcttag aaaaacgttg ttaattaata caaacatggc ccccgcaagg gttaacggca
    16741 ttaagcgaat aaagccattt ttgattgcat taacgtgtgg ctgctttcct attttaactg
    16801 caaaagggag gagtaccttt tcaagcgatg caataacatt actcatagaa aaataccctt
    16861 aaaaaccgca atttaaatat tacggtattg atttatgaaa taactctttg acgggaaaat
    16921 ttaaaataaa ttaatttgct gcggcttttt taatcgctgc aaccgcagcc ttaagcacgc
    16981 ctaaaccatc gactttgcca taaagcagcg agtcaattac ttcaaccggt ttgttgggta
    17041 acaaacgctg gatttcgggc aacatataag caatctgcgg ccctaataac acgacatcgg
    17101 cattctgacc tttttcacca gccagtgttt ccggaaatgc ttcaataatg accggaactt
    17161 catatttttc tgcctgtgcg cgcatttttg ataccagtaa agagatagac atgcccgcag
    17221 aacaaaacag ataaatgtgt ttcttttcca taaaactgcc ctcatcgacg attatctgtc
    17281 agccagacac tccgcaagcc ttgacctgct tccatgctct gggtaacttg cgaaaccaac
    17341 atgacttttt ttgtggctga aaggagtata cggtatagac ctgagcaata gtatttcttt
    17401 gaccttataa attgtttctc cttgacctgt ggttatgacc ctcttcacat ttcgggcaaa
    17461 taattcgcga agataattaa gaactcaggc caaaaaaaac cggcgcaatg gccggtttcc
    17521 gttgttactc aagctttcag acgaattgat tacttcgcag cctgtggatc agtgtcgtat
    17581 tcagcacagg tctgataacc ggagttgatg acatgtccgg tatcatctaa cgcgacaaag
    17641 taggtttctg ctttaccatc acgttgaccc aggatgtagg tctggcaagt accgcgagca
    17701 tggatcatgc tcacttcaga cgaaggttta cccgcaattt gtgcaacctg cgcccggctc
    17761 atgccttttt tgacgtcttt caccacaggc tgtacaaact ggtctttggt acgatcataa
    17821 gctgtacaac ccgccagcat ggttaatacc gccgctgcac tcagaattcc tgccatattc
    17881 ttgttcatat tccgtcctct tgtttatcag cgtgttagat aagcctggaa tacattgggc
    17941 gctttttcaa gcccgtgaac gaaacggctc cgctttcaga ggattcctgt atgacgtttt
    18001 aaccaccatt cagcccgctg tcgcttgtcg tttcagtagc aacgggttag ctttaaggaa
    18061 gttttgtctt ttctgtctgg aggggttcaa tgacattgca acaacaaata ataaaggcgc
    18121 tgggcgcaaa accgcagatt aatgctgaag aggaaattcg tcgtagtgtc gattttctga
    18181 aaagctacct gcaaacttat ccgttcatta aatcactggt gctcgggatc agcggcggtc
    18241 aggactccac gcttgccgga aagctgtgcc agatggcgat taatgagctg cgccaggaaa
    18301 ctcgcaacga atcactgcaa tttattgccg tacgcctgcc ctatggtgtt caggctgacg
    18361 aacaagattg ccaggatgcc attgccttta ttcaaccgga tcgcgtatta accgttaata
    18421 tcaagggcgc ggtattggct agcgagcagg cattgcggga agcaggcatt gaactgagcg
    18481 attttgtccg tggcaatgaa aaagcgcgtg agcggatgaa agcacaatat agcattgcgg
    18541 gtatgaccag cggtgtcgtg gtgggcaccg atcatgcagc agaagccatt accggattct
    18601 tcactaaata tggtgacggc ggtacggata ttaatccgct gtatcgtctc aacaaacgcc
    18661 agggtaaaca gttactgacg gcattaggtt gcccggaaca cctttataag aaagccccaa
    18721 ctgccgatct tgaagatgat cgcccttctc tgccagatga agtggcactc ggcgtgacct
    18781 atgacaatat cgacgactat ctggaaggga aaaacctacc tgaacaggtc gccagaacaa
    18841 tagagaactg gtatctgaaa accgaacata aacgccgtcc gccaattaac gttttcgatg
    18901 atttctggaa aaagtaataa tttgcaccta ttggattgtg cgaaaaagag taatttgttc
    18961 acgccggatg cggcgtgaac gccttattcg acctacaaaa ctatgcaaat tcaatacatt
    19021 gcaggagtcg aataggcctg acaggcgtag cacgtcagac ggtgtaacct ttgtcatcaa
    19081 cccgcctctt ttttaatcgc ctcccgcctg ttacactgga tagataacca gcattcggag
    19141 tcaacagtgg tacggcgttt aacttctccg cggctcgaat ttgaagctgc ggcaatttat
    19201 gaatatcccg aacatttaca ttcattcctt aatgacttac ccacccgacc aggggtgtat
    19261 ctgtttcatg gcgaaagtga caccatgccg ctctatatcg gcaaaagcgt taatatccgc
    19321 agtcgggtgc tttctcattt acgcaccccg gatgaagccg ccatgctacg gcaatcccgg
    19381 cgaattagct ggatctgcac cgcaggcgaa atcggcgctc tgctccttga agcgcgatta
    19441 atcaaagaac aacagccgct gtttaataaa cggttgcgcc gcaatcgcca gctctgtgcc
    19501 ctgcaattaa atgaaaagcg cgtcgatgtg gtgtatgcca aagaggtgga tttttcacga
    19561 gcccccaacc tgtttggcct gtttgccaat aggcgcgcag ctttgcaagc attgcagagc
    19621 atcgctgatg aacaaaaact ttgttatggc ctgttgggac tggaaccgtt aagtcgcggt
    19681 cgtgcatgtt ttcgttcagc gctaaaacgt tgcgccggag catgctgcgg taaagagagc
    19741 catgaggaac atgcgctacg cttgcgccag tctctggagc gtttgcgagt ggtgtgttgg
    19801 ccttggcaag gtgcggtggc actgaaagaa cagcacccgg aaatgactca atatcatatt
    19861 attcaaaact ggctgtggct gggggcggtt aattcgctgg aagaagcgac aacgttaatt
    19921 cggacacccg ccgggtttga tcacgacggt tataaaattc tttgtaagcc gctgctttcc
    19981 ggtaactatg aaattactga acttgatccg gcgaatgacc agcgagccag ttgatttcac
    20041 tgaacaacag tttaccttgc ggttgtaaaa gccgcagggt gtgcctgcca tcgaaccagc
    20101 acgcgccctg gtcggtagtg agcagtttgt cgcccagttg ccacgccccg ttaatcacaa
    20161 acaccacccc gccgcgtgag ccaaaggtag taaacgtacg ttcggcaatt ctgactttcg
    20221 ccttacagac atccaggcgc gtcataatat tgaaatccat cgacatctgc cctgccgtca
    20281 gtttcgcttt taccacctgg tccgccgtaa aggcaaaagg ctgtaacggt tttaaggtat
    20341 ggttaaagcg gtctgcgctt tcaaggagca tctcgccgcc ttccagcaac gtcactatcc
    20401 tttccatgcc gggaaacaaa gaaaactccc cattcgccgc gatggatgca atgctggcac
    20461 gccagtaaaa atcacgtttt gccggtggga aagtgcaaat ttcgcgcgtt tcaccggcag
    20521 cgtttcgcca cagattcacc gacattttac gcatatcaaa gtattccata ctcgctcccg
    20581 gcccgttctt ttcattgtta tcgttcaggt aaccgtggat gattatcggc aactcgggtg
    20641 atttacgtta gtggtgatca ggcttttttt acaactgttt gagaagagaa aagaaaaccg
    20701 ccgatcctgt ccaccgcatt actgcaaggt agtggacaag accggcggtc ttaagttttt
    20761 tggctgaaag attattcagc agttgcaggc attttacctt ttgccgctgg acgttctgtc
    20821 agacgcttct caaaattagc attaaattgc tttttctgtt ccggcgtcag gatgttgtaa
    20881 attttgttct gggtttccat gtgcgccagc atgttagctt tgcgctgttc ttccattttt
    20941 gcgatctgcg cttcagcttt tgctttatcg aaggtatcgc tggcaatgat gtcatgcatt
    21001 gcgcggcgtt cttccagcgg cggacgtttc atctggtcac gctggccttt catgatttcg
    21061 tggatctgct gtttctgcgc gtcggtcagg ttcaggtctt tgaacatcat gtcctgatgc
    21121 ggaccgaact tgcctttgtg gtgcatcatc ggcttcgcgt cagccggtgc tgcggtagtg
    21181 gtgtctgcgg catgggccag gttagccgcg ccaagagcca gggtagaggc aacaaacagt
    21241 gcagttaatt tacgcatatt ctatatcctt cctttcagtt atttattacg gctttcttaa
    21301 gtagcgtgcc gtgttgacga gattaacttt actgacttta gcgtcaatta atcagagcaa
    21361 cggtaaaaca atgaaagtgt aaaaaacact tttgcgccaa ttatggagaa aaaaagaaaa
    21421 tttgatggag agtgatgaga gaatattaca acacgatgat tttgcagaga ttatgaagaa
    21481 ctataccgga tgactggtga taaataaagc aaataaccag gattaatctg tattaattta
    21541 taagaaagca acttaatacc cgcagaatga tttctgcggg taagtattag cttatttttt
    21601 cgagcattaa tcccgcgcgt aatcccaacg ctaccaacgg attagggaat aacacatact
    21661 ctacatcatg ggttacggta aaacgttcct ctccgtcctg cgccagcaat gttcctttct
    21721 caaacggcat aaaattcagc gtgtcacttg ccatatgcat ttcgaaggac ggcgagtggc
    21781 gagtaatttg cgaaaccacc cgataacgga gcggcggtgt tctcacgata ccgacactct
    21841 caccagatag cagcgcagca attgcgctgg cagttactgc aaactggcga agatcgtttt
    21901 gcccaaaggg caacgctttg ccaagttcca acgtacagac cagcgcgcca aaatgtctga
    21961 cgctgaaatg ggtaaacgta ccaccaggct cctgatggaa caccagcgcc tccagccccg
    22021 ccgcacccag ccacgtcaga aatttctcgt cccagggaat gtcgcgttgc ggtaatacac
    22081 cgaactgcgg atgcaaggag ccacgaattg cggtatgtag atcaaggtgc caacgcacag
    22141 attctttgcc ctggtcataa aaatcttcca ggcactgttc cagttcgcgc gcccgacagg
    22201 tttctccgct ttcagcaaat agctgccaac gaccgccaaa cattcggttc atatcgctat
    22261 ggcaataacg tttcccttgc ttcagcgcag gaggattccc gaggatcacc agcaaccgcc
    22321 aacgtaacgg gatctcgccg tgagatatcg cgccaagcaa cgcgtccagc atttccacag
    22381 gtgccgtctc attaccgtgg atccccgcag aaatcaccag cgcgccttgc ggtggcgtta
    22441 atggcgtcag ttccagcaca ccatcgccca gccagcgcca gcgaacgccg ttgatttctc
    22501 gctcggtgat aaccggtttt ttacccgtta aggtcagagc aagaaaatta tccattgccc
    22561 cctccctcgc gctggaacgg ataaaccgaa ccgagattca gtaattggct caatacatcc
    22621 agtgcttccc gcccttcgcg cagcaattgc gggtcggcca gatcggcagc agtaagacga
    22681 tcgcggtagt aacgatccac ccagtcattg agcacattaa acaaggtatc gttcatcatc
    22741 accgccggat tcaccgcccg gcgttcttct tctgtcaata ccccccgcaa ccgcaggcac
    22801 gccgggccgc cgccattcgc catgctttca cgtaagtcaa agacttttag ttcactaatc
    22861 gggttgtcag cggcaaggag ttcattgaga taaccccata ctccggcgtg ctcccgacac
    22921 tcctgaggca gcaccaacat catggaacca tcatcgcggc tcaacagttg gctgttaaac
    22981 agataggtag acaccgcatc cgacacggaa acctgagttg ccggaacttc tatcgccata
    23041 aaaccgttca cccgcgtgcc agtaactgtg actggcgagc gaacgcctgt tggtggcaaa
    23101 acagcacctg gcggttactc acggcaatca cgtcattatg aaaaacgctc tggtcgataa
    23161 cgtccgggtt ttgctgggcg aaaatcacct gttggggatt cacctgatta agccttgcca
    23221 ccgcctcgct ggcttcgcga gtctgtcgcg ccggataacg ggaaggccgg gtatcattgc
    23281 cttcttctcg cccgtagaca aaaagtagca tacccggttc accgtaatga ccgccgagac
    23341 gattgtggtt tgccgccccc tcatcaccga gcaacgctac ctgcggcaac gccgaatgga
    23401 cgctaaattt ctcttcgtcc ttaaaaatcg cttttaacag cgattcagtg acgggcgctt
    23461 ccagcgaacg gtgaaattta ttgttcaggt tggcaacggt gagatgcact ttgccatcca
    23521 gcgtatcggc agatggcgcg atcgttgccg cattggctac ccacattggc gaagcggaac
    23581 tgacgctgga aagccagtgc ggtgcctggc gtgcaacttt ttccagtacc tgctcatcgc
    23641 taccactgaa tcccaactga cgcagcaccg gaataaacgg acgctcgtgc ggcgggatca
    23701 cggcctgggg gaatcccgca tcggcaaggg ctttcatttt cagtaagccc tgttttgccg
    23761 ccagtcgcgg gttagacacc tgaaaacggt gacgggtaga ggcttcatta ccaaacgaca
    23821 ggcccgcgta atgatgcgtc agccctacca gcccgtcgaa attaacttcc caggcgttca
    23881 tcgcaccacc tcatcggaaa aatccagccc ggggttaagc gtggcgggca atgttaacga
    23941 gtccgactcc aggctcgcca taggccatgc gcagtaatct gcggcatacc aggcgctggg
    24001 gcgatggtta ccggaagcac caatgccgcc gaatggggcg gtactggcag caccagtaag
    24061 cggtttgttc cagttaacaa tccccgcccg cgcctccagc aacagttgat cgaatttttc
    24121 ccgctcgggg gaaaccagac cgcaaaagag tccgaagcga gtgttgttcg ccatccgaat
    24181 cgcctcatca aaagtatcat aacgccagac gcgcaaaagc ggcccgaaca cctcttcatc
    24241 cggcacaccg ccaacgcccg tcatttcaat gatccctggt gtcagcaacg atgtccctgc
    24301 ttgtaataag cgcggcgcaa gcagggttcg tccgcccatc gcttccagtt gctgccaggc
    24361 agtaaccacc tgctgtgcgg cctgttcaga aatcagcccg ccaataaacg gctgcggttc
    24421 gtcatcccag ttgcccggcg ttaatcgctg gctgacggca accagactag caagaaacgc
    24481 atcgccctgc gctccgcttt tgagcaataa acggcgggcg caggtgcagc gttgcccgac
    24541 tgtgacaaac gccgactgaa tggtcagatg gacagccgcg tcgatatccg ccacctcatc
    24601 gataattagc ggattattac cgcccatctc aagggcgaga attttctccg gctgaccgga
    24661 gagctggcga tgcagctggt agccggtatt ggcgctaccg gtaaacagca aaccgtcgag
    24721 atcctccagc gcactcagcg cctgacccgt ttcacgcccg ccctgcacca ggttcagcac
    24781 gcccggcggc aagccagcct gctgccataa acgcattacc gcttcgccac tccacggtgt
    24841 cagttcgctg ggtttaaaga taatggtgtt acctgccagc aatgccggaa cgatatgtcc
    24901 gttcggcaaa tgaccaggga aattatacgg cccaaacacc gccagcacgc cgtgcgggcg
    24961 atgtcgcagg ctcgccgcac cgtccggcat ttcactacgc tgctcgccgg tacgaacgtg
    25021 atacgcctta attgatatcg cgattttatt gatcatcgcc gtcacttcgg ttgccgcttc
    25081 ccagcgcggc ttacccgttt ctctggcaat aatcgcggtt aattcggctt tattgctttc
    25141 cagcaatccg gcaaagcgtt cgacaacgac ctgacgttca gccaatgaga gccgcgccca
    25201 gcgcggaaac gccgcacggg ctgcccgaca agcctgttcg acctgagcgg catcggcatc
    25261 attgccttgc cataacacct cgcccgatac cggattacgc ttcacacgcg atgcgccctg
    25321 gcccgttatc cagtcaccgt taatccataa agtcatgctg ttttctcctc tgcgcacagg
    25381 cgcaccagac gaacgcgatc ccctgcgtgg catttgaggg catccagttg tgcggcggtt
    25441 aaaatcaaac gctcggttgc cggatcggta cgcgccagca ccacgcggaa atggtgataa
    25501 ttttcattgg cgaccaggca ggccggaaaa tcgccctgcg caggctgccc ttctgccact
    25561 tccaccagcc gacttttacg gatggcgcgc acgcggtcga tgtcacactc aagcgttggc
    25621 ccgccgtcga agatgtcgat atagttgcgg taacgaaaac cttctttctc cagcaccgcg
    25681 cgggcaggcg cggtttgcgg atgtacctga ccgatgacgt cctgggcttc ctgggataaa
    25741 aagtgggtat agatcggatg tttcggcatc agttctgcaa taaatgcctt ttgcccggtg
    25801 ccgcagagaa aatcggcgcg gctaaaatcc atcgaaaaga agcgtttacc gaggctttgc
    25861 cagaacggtg aatagccgtg ttcgtcaatc accccgcgca tttcagcaac cactttgtca
    25921 ttaaacttgt cgcgaaaagc cgccataaac ataaagcgcg atttcgacag caaatagccg
    25981 ttgccctctt tgcgccagtc cgggtcgaga aacagcgtgc acagctcgct gctgccggtg
    26041 tgatcgttac taagaaacag cgtcggcaat gcgttataga cattcagctc ttttgaggcg
    26101 tgaaccaacg tgccgacgcg atagttgtac cagggatcgt tcagcccaac cgccacctca
    26161 atagcgcaaa tccccgccac ggtgcctgtc tcgctatctt ccagcacgaa cacatagccc
    26221 tgctcgcttt tgggcagttc accttgccag gttttgattg ccctttcgat acgcgccgaa
    26281 agcgtggctt cattggcggg aagcgacgtc aggccgccgc ccgttttgct ggcaagctgc
    26341 atcagcgccg agacatctga gcgctcaacg ggacggatga ccatcatgat gaacctcggc
    26401 taacaaagtg ttcgcaagca gctgcaaagc gatccagtcc ggtcgtcacc tcttcttcgc
    26461 tgacattgag cgcaggcgca aaacgcacca cgttgccacc cgcaatcagt accatcacgc
    26521 ctgctttcgc cgcttccaga gagatctgtt tcgcttgccc ggcgtaatcg gcattcagta
    26581 cacagccaat caacaaacct aagccgcgaa cttcactgaa caaaccatag cggtgattaa
    26641 tagtattaag acgctcaaca aaccagtcgt gacgctgttt aacgccatta agcatctctg
    26701 gtgtgttgat gagctccagc actttgcctg ctaccgccga ggccagcgga ttaccgccat
    26761 aggtggtgcc atgagtgcca acggtcatca cgcgggcgca ctcttcggtt gccaacaacg
    26821 caccgaccgg gaaaccgccg cccagcgctt tggcggtggt taacagatcg ggcgtcacgc
    26881 cgtagtgcat ataggcatac agctcaccag tgcgcccgac gccggtttgt acctcatcaa
    26941 aaatcagcaa ggcattgtgg cggtcacaca attcacgcag accttgtaaa aacgcgttgc
    27001 tggctggcac cacaccgcct tcgccctaga tgggctcgac aatcaccgca caggtggcgt
    27061 cgtcaatcag cgcgctggca gagttaatat cgttatatgc agcatgacga atatccgccg
    27121 gcagtggcgc aaaatcctgt gaataggctg gctgcccacc cgcactgaca gtaaacagcg
    27181 tgcgaccatg aaacgcattt ttgaacgcca cgatgccact cttatggctg ccgtagcggt
    27241 cgtgagcgaa tttacgcgcc agttttagcg ccgcttcgtt ggcttccgca ccggagttac
    27301 aaaagaagac gcgatcggca aacgtggcgt cgatcaattt tttcgccagt cgcagtaccg
    27361 gctcgttggt gtaaccgttg ccggtatgcc agaacttact cgcctgttcg ttcagcgctt
    27421 cacgcagttc cggatgcgca tggcccagcg cgttcaccgc aatgccaccc gcgaagtcga
    27481 tatactcttt cccctgctga tcccacaagc gcgaaccttc gccacgtacc ggtataaagg
    27541 gtgccggagc gtaaacaggt atcatccatt catcaaagtt ttcacgcgta attggctgag
    27601 acatagcgac ctctacagta aataattcgt tatttgcatg ttaacaataa gtaatgtttg
    27661 cgctgtaaat gtagattgca gggttcgtgc cagccagtga taaaagtgca taaacggcgg
    27721 aggctaactg gaaatcaagg agttataacc aaaccatatg catttaaagt gcatataaag
    27781 tgaatacgtt tgcgatgtgg gtgaataaaa agaataaaaa acgcaatgtt atgcagaagt
    27841 aaaatataat tctagaattg tgatcattga cgaaatttac tggaaattac tgcgccattc
    27901 tgacgcagcg cgcaccaaaa gcgggcattt tttgcgccat cattgacatc attaacaacc
    27961 atcgatcaaa tcacttaaca acaggcggta agcaacgcga aattctgcta ccatccacgc
    28021 actctttatc tgaataaatg gcagcgacta tgaaatttgt ctcttttaat atcaacggcc
    28081 tgcgcgccag acctcaccag cttgaagcca tcgtcgaaaa gcaccaaccg gatgtgattg
    28141 gcctgcagga gacaaaagtt catgacgata tgtttccgct cgaagaggtg gcgaagctcg
    28201 gctacaacgt gttttatcac gggcagaaag gccattatgg cgtggcgctg ctgaccaaag
    28261 agacgccgat tgccgtgcgt cgcggctttc ccggtgacga cgaagaggcg cagcggcgga
    28321 ttattatggc ggaaatcccc tcactgctgg gtaatgtcac cgtgatcaac ggttacttcc
    28381 cgcagggtga aagccgcgac catccgataa aattcccggc aaaagcgcag ttttatcaga
    28441 atctgcaaaa ctacctggaa accgaactca aacgtgataa tccggtactg attatgggcg
    28501 atatgaatat cagccctaca gatctggata tcggcattgg cgaagaaaac cgtaagcgct
    28561 ggctgcgtac cggtaaatgc tctttcctgc cggaagagcg cgaatggatg gacaggctga
    28621 tgagctgggg ggtggtcgat accttccgcc atgctaatcc gcaaacagca gatcgtttct
    28681 catggtttga ttaccgctca aaaggttttg acgataaccg tggtctgcgc atcgacctgc
    28741 tgctcgccag ccaaccgctg gcagaatgtt gcgtagaaac cggcatcgac tatgaaattc
    28801 gcagcatgga aaaaccgtcc gatcacgccc ccgtctgggc gaccttccgc cgctaattta
    28861 gcagctctcc tggctcaaac tgggtcagga gaattaacct tgagaaaaat caacaaactg
    28921 tcagtaatga tttgttgcct gccgtccttt gttataccgt cactgcgttt ttagttgtct
    28981 gaccacttct ctattatcaa gtttgatata ggaaactcca cgatgaacgc tgagcgtaac
    29041 tttctgtttg cctgtcttat ttttgcgctg gtcatttacg ctatccacgc tttcggttta
    29101 ttcgatctgc tcaccgattt accccactta cagacactca ttcgccagag cggattgttc
    29161 ggttatagcc tctatattct gttattcatc aatgccccct ctttctgtta ccaggaagca
    29221 tactggtgat cgctggagga atagtttttg gcccgctctt aggacacaac tctcattaat
    29281 tgccgcaacg ctggcctcct cgtgctcatt cctgctggcg cgctggctgg gacgtgattt
    29341 actgctgaaa tacgtgggcc atagccatac cttccaggcc attgaaaaag gcattgcgcg
    29401 taacggtatt gattttctta ttctgacccg cttaatcccg ttgtttcctt acaatattca
    29461 aaattacgct tacggattca ccaccatcgc cttctggcct tataccctta tttcggcact
    29521 cacgaccctg cccggtattg ttatttatac cgtgatggca agcgatctcg ccaatgaagg
    29581 cattacgctg cgctttattt tacaactctg tctggcgggc ctggcgctgt ttattctcat
    29641 ccagctcgca aaactctacg cccgacacaa acatgtggat ctgtctgctt cgcgccgcag
    29701 cccacttact cacccaaaaa atgaaggata gaacgatgtc gcaacattat tcagtgtcat
    29761 ggaaaaaagg actggctgca ctctgcttac tggctgttgc cgggcttagc ggctgcgatc
    29821 aaaaagagaa tgcggcggca aaagtggaat acgacggact ttcgaacagc caaccgctgc
    29881 gcgtcgatgc caataaccat acggtaacca tgctggtgca aattaatggt cgtttcctca
    29941 ccgacgacac tcgtcacggt attgtgttta aagatggctc caacggacat aaatcgctgt
    30001 ttatggctta tgcgcccccg aaagcatttt atgaagccct gaaagaggca ggtggtacgc
    30061 cgggcgaaaa catgacgatg gataataaag aaacgactca tgtcacaggc agcaaactgg
    30121 atatttcggt caactggcaa ggggcggcaa aagcgtattc cttcgatgaa gtgattgttg
    30181 acagtaatgg caagaaactg gacatgcgct ttggcggtaa tttaacggca gcagaagaga
    30241 agaaaacggg ttgcctggtg tgtctggata gctgcccggt cggcatcgtc agcaatgcaa
    30301 catacactta tggtgcggtg gaaaaacacg gcgaagtgaa attcaaaggc aatgcctcag
    30361 ttctcccggc ggataacacg ctggcaacgg ttacctttaa aatcaccgaa taaagccaag
    30421 ataaaggatg atgaagatac aatcgcgtaa aatctggtac taccgtataa ccctcatcat
    30481 cctgttattc gctatgctgc tcgcatgggc gctgcttccc ggcgtccatg agtttatcaa
    30541 tcgcagcgtt gcggcgtttg ccgccgtgga ccaacagggt atagaacgct ttattcagtc
    30601 ttacggcgca ctggcggcag ttgtctcgtt cttgttgatg attttacagg ccattgctgc
    30661 accgctacct gcgtttttga tcacctttgc caatgcgtcg ctgtttggcg cgttctgggg
    30721 cggcttgctg tcgtggacca gttcgatggc cggcgcggcg ctgtgctttt ttatcgccag
    30781 agtgatgggc cgcgaagtgg tggaaaaatt aaccggcaaa accgtgcttg acagtatgga
    30841 cggctttttc actcgctacg gcaaacacac catcctggtc tgtcggttat tgccttttgt
    30901 ccctttcgat ccaatcagct atgctgccgg tctgacttca atacgttttc gctcgttttt
    30961 tatcgccacc gggcttggtc aattaccggc gactattgtt tattcctggg caggcagcat
    31021 gttaacaggc ggtactttct ggtttgtcac cgaactgttt attctgtttg ccctgaccgt
    31081 ggtgattttt atggcgaaga aaatatggct tgagcgccag aagaggaatg actgatgggt
    31141 ttaccgccgc ttagcaaaat tcctttcatt ttacgtccgc aggcgtggct gcatcgtcgc
    31201 cattacggcg aggtgctaag cccgattcgc tggtgggggc ggatcccgtt tatcttttat
    31261 ttggtgtcaa tgtttgttgg gtggctggag cgcaaacgct caccgctcga tccggtagtg
    31321 cgatcgcttg tcagcgcgcg cattgcgcaa atgtgcctgt gtgagttttg cgtggatatc
    31381 accagtatga aagtcgccga gcgtaccggc agcaccgata aactgctggc agtggctgac
    31441 tggcggcaaa gcccgctctt tagcgatgaa gaacggctgg cgctggagta cgccgaagcc
    31501 gcaagcgtca caccgccaac agtcgatgat gccctgcgta cccgactggc tgcgcatttt
    31561 gacgctcagg cgctcaccga actgacggca ttgatcggcc tgcaaaacct gtcggctcgt
    31621 tttaattctg ccatggacat tcccgctcag gggctgtgcc gtattccgga aaaacgttct
    31681 taaggaggga agatgcgcca ttgtgggtgg ttgctgggat tgttatcgct gttttctttg
    31741 gcaacacatg ccagtgactg gcaggaaatt aaaaatgagg ccaaagggca aaccgtctgg
    31801 tttaacgcct ggggcggcga taccgcaatt aaccgctatc tcgactgggt tagcggcgag
    31861 atgaaaaccc attacgctat aaacctgaag attgttcgtc tcgcggatgc cgcagacgcg
    31921 gtgaagcgca ttcagaccga agctgctgcc ggtcgtaaaa cgggcggctc ggtggatctg
    31981 ctctgggtga acggcgaaaa cttccgcacc ttaaa
//
LOCUS       DAJTQY010000041        31767 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.041, whole genome shotgun sequence.
ACCESSION   DAJTQY010000041 DAJTQY010000000
VERSION     DAJTQY010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31767
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.041"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(<1..1357)
                     /gene="yeaG"
                     /locus_tag="OO349_001964"
     CDS             complement(<1..1357)
                     /gene="yeaG"
                     /locus_tag="OO349_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HCR5867044.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQDPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNKSEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQ"
     gene            1793..2539
                     /gene="mipA"
                     /locus_tag="OO349_001965"
     CDS             1793..2539
                     /gene="mipA"
                     /locus_tag="OO349_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HCR5867045.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            2629..3390
                     /locus_tag="OO349_001966"
     CDS             2629..3390
                     /locus_tag="OO349_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCR5867046.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLREKVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLGYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHV
                     QQHAAALEVELSSAELAMLDKAYPAPKGKTALDMV"
     gene            complement(3441..4325)
                     /locus_tag="OO349_001967"
     CDS             complement(3441..4325)
                     /locus_tag="OO349_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HCR5867047.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHHEDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(4409..5404)
                     /gene="gapA"
                     /locus_tag="OO349_001968"
     CDS             complement(4409..5404)
                     /gene="gapA"
                     /locus_tag="OO349_001968"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCR5867048.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            5746..6159
                     /gene="msrB"
                     /locus_tag="OO349_001969"
     CDS             5746..6159
                     /gene="msrB"
                     /locus_tag="OO349_001969"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HCR5867049.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            6201..6473
                     /locus_tag="OO349_001970"
     CDS             6201..6473
                     /locus_tag="OO349_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HCR5867050.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            6843..7919
                     /locus_tag="OO349_001971"
     CDS             6843..7919
                     /locus_tag="OO349_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR5867051.1"
                     /translation="MKALARFGKAFGGYKMIHVPQPICGPEDVVIEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDMVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            7946..9325
                     /locus_tag="OO349_001972"
     CDS             7946..9325
                     /locus_tag="OO349_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5867052.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMDVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRQTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLITM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSNIVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            9342..10387
                     /locus_tag="OO349_001973"
                     /pseudo
     CDS             9342..10387
                     /locus_tag="OO349_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
     gene            10408..11244
                     /locus_tag="OO349_001974"
     CDS             10408..11244
                     /locus_tag="OO349_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HCR5867053.1"
                     /translation="MLADIRYWENDATNKYYAIAHFNVWNAEMLMGVKDAAEEAKSPV
                     IISFSTGFVGNTSFEDFSHMMVSMAQKATVPVITHWDHGRSIEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIELFGSDGK
                     AE"
     gene            11249..12196
                     /locus_tag="OO349_001975"
     CDS             11249..12196
                     /locus_tag="OO349_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HCR5867054.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMSTGGDA
                     INEATIISRLGHRTALMSRIGKDASGQFILDHCRKENIDIQSLKQDVSIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFARFPQAKLLSLASIFNSPLLDGKALTEIFTQA
                     KARQMIICADMIKPRLNETLDDICEALSYVDYLFPNFAEEKLLTGKETLDEIADCFLA
                     CGVKTVVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNL
                     RECARFANATAAISVLSVGATTGVKNRKLVEQLLEEYEG"
     gene            12206..13186
                     /gene="ydjG"
                     /locus_tag="OO349_001976"
     CDS             12206..13186
                     /gene="ydjG"
                     /locus_tag="OO349_001976"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HCR5867055.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTALGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNELKAE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEALER"
     gene            13323..14081
                     /locus_tag="OO349_001977"
     CDS             13323..14081
                     /locus_tag="OO349_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR5867056.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAVLTENIHFYKRASSFYEEKLLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            14199..15557
                     /locus_tag="OO349_001978"
     CDS             14199..15557
                     /locus_tag="OO349_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5867057.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLYAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFFRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLEGKGQIAGAESQLR
                     EVEQQIEREKSIRLPQLTLNQSNSKVKVIKGTFRLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSALLI
                     IIAVLGYIYSSQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLVLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(15650..16291)
                     /gene="pncA"
                     /locus_tag="OO349_001979"
     CDS             complement(15650..16291)
                     /gene="pncA"
                     /locus_tag="OO349_001979"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HCR5867058.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVEVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLGGLRQTFWPEHCVQNSEGAQLHPLLNQ
                     KEIAAVFHKGENPLVDSYSAFFDNGRRQKTALDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(16302..17318)
                     /gene="ansA"
                     /locus_tag="OO349_001980"
     CDS             complement(16302..17318)
                     /gene="ansA"
                     /locus_tag="OO349_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HCR5867059.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMSGYATGNALAHAGVIDGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(17485..19341)
                     /gene="sppA"
                     /locus_tag="OO349_001981"
     CDS             complement(17485..19341)
                     /gene="sppA"
                     /locus_tag="OO349_001981"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HCR5867060.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKSVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            19502..20053
                     /locus_tag="OO349_001982"
     CDS             19502..20053
                     /locus_tag="OO349_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HCR5867061.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGCERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCKENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            20170..21213
                     /gene="selD"
                     /locus_tag="OO349_001983"
     CDS             20170..21213
                     /gene="selD"
                     /locus_tag="OO349_001983"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HCR5867062.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDLNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYEAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKTTAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            21218..23179
                     /gene="topB"
                     /locus_tag="OO349_001984"
     CDS             21218..23179
                     /gene="topB"
                     /locus_tag="OO349_001984"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCR5867063.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKVLRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
     gene            23306..24346
                     /locus_tag="OO349_001985"
     CDS             23306..24346
                     /locus_tag="OO349_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR5867064.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWGIFNSNFERVIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLENQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIALLNKFAVF
                     PSDYISDCANNRSGKSSAKLIWAAELSWMISISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(24463..25806)
                     /gene="gdhA"
                     /locus_tag="OO349_001986"
     CDS             complement(24463..25806)
                     /gene="gdhA"
                     /locus_tag="OO349_001986"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HCR5867065.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQIQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGKVMRFCQTLMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMSVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            26042..26314
                     /locus_tag="OO349_001987"
     CDS             26042..26314
                     /locus_tag="OO349_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HCR5867066.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            complement(26280..26687)
                     /gene="nudG"
                     /locus_tag="OO349_001988"
     CDS             complement(26280..26687)
                     /gene="nudG"
                     /locus_tag="OO349_001988"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HCR5867067.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVELDES
                     QQQVLVRELNEELDIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
     gene            26774..27394
                     /locus_tag="OO349_001989"
     CDS             26774..27394
                     /locus_tag="OO349_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCR5867068.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            complement(27403..28710)
                     /gene="ynjE"
                     /locus_tag="OO349_001990"
                     /pseudo
     CDS             complement(27403..28710)
                     /gene="ynjE"
                     /locus_tag="OO349_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
     gene            complement(28777..29430)
                     /locus_tag="OO349_001991"
     CDS             complement(28777..29430)
                     /locus_tag="OO349_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR5867069.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRALAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            complement(29430..30947)
                     /locus_tag="OO349_001992"
                     /pseudo
     CDS             complement(29430..30947)
                     /locus_tag="OO349_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(30920..31767)
                     /locus_tag="OO349_001993"
                     /pseudo
     CDS             complement(30920..31767)
                     /locus_tag="OO349_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
BASE COUNT         7959 a         7605 c         8240 g         7963 t
ORIGIN      
        1 gctgttccag gacgtagaac agatggaccg ggtttgccgc cacttctaca tgatcgaagt
       61 tgaacacgcg ggagaggatc ttaaacgcaa aacgcgtcga cagaccgttc atcccttcat
      121 cgacaccggc gtagtcacga tattcctgat acgacttggc tttcggatcg gtgtctttca
      181 gactttcgcc atcataaacc cgcatctttg aataaatgct ggagttttct ggctctttca
      241 ggcgcgaaag aatggaaaaa cgtgacagtg tttcgagcgt gccaggggcg catggggcgt
      301 gagtcaattc actgtgatta agcaatttct cgtagatttt gatctcttcg gaaatgcgca
      361 agcaatacgg caccttcacg atgtaaacac ggtcgaggaa ggcttcgttg tttttgttat
      421 tacggaaagt gacccattcg gacttgttcg agtgggcgag aataatcccg ttgaacggca
      481 gggcggagat accttccgtc ccgttgtagt taccttcctg agtggcggtt aacaagggat
      541 gcagcacttt tatcggtgct ttaaacatct caacgaattc catgatcccc tgattggcgc
      601 ggcacagcgc accggaatag ccgtaggcgt ccgggtcatt ctgcgcgtag tgttcgagtt
      661 tacgaatatc gactttccca accagcgcgg agatgtcctg gttgttctca tcaccgggtt
      721 ccgttttggc gatagcaatt tgttgcagaa ttgacggcca gaccttcact acccggaact
      781 tagtgatatc gccaccaaat tcatgcaggc gttttgccgc ccatggcgac atgatggtgc
      841 cgagataacg gcgaggaatg ccatactctt tttccagaat ctgagcatct tcctgcggat
      901 tgaaaagaca gaacggatga tcgttgaccg ggctacgctc accgttggcg ctcaatacat
      961 aaatcggtac gagctgcatt aatgatttca gtcgctcagc aagcgatgat ttacccccac
     1021 ccacaggccc cagcagatac aggatttgtt tcttctcttc cagcccctga gccgcgtgtt
     1081 tcagataaga gacaatctgt tcaatcgcgt cttccatgcc gtaaaactct tcaaatgccg
     1141 gataacgtgc aatgacccgg ttagaaaaga gtcgagaaag tctggggtcc tgggctgtat
     1201 cgaccatgac aggctcaccg atagccatca atagccgctc agccgcgttg gcataagcac
     1261 tgcgatcttg ccgacaagtg gtaagaaact cctgcagtgt gaactcttcg tccttggcag
     1321 cttcatatcg ctggcgatag tgatcaaata tattcatggt atgccgtcct ttcgtttttt
     1381 agcacaggtt aagagccgtt catatgaata gtagaggctc ccggaagagg taaactgaac
     1441 gacgtctctt gctaaaacac agcaactctc atgccatttt atcgacgatg agaacaacgt
     1501 gcccggtgat acaccttcaa aattaagcgt agatggcaaa tggaaaactt gcctgcacgg
     1561 ctttcttaat ttcaatggca tatcaacaac tcatccataa attttgcata attaatgtaa
     1621 agaccaggct cgccagtaac gctaaatcca tttggctgta agcgcggtgt catccgcggc
     1681 aggaaaatta aacagttact ttaaaaaatg aaaacgtaaa aaggttgggt ttcgatgtat
     1741 tgacgggtaa actttgtcgc ccgctaaaca tttgtttaag gaatgattaa ttgtgaccaa
     1801 actcaaactt ctggcacttg gagtgcttat cgcaacgtct gcaggcgtag cgcacgctga
     1861 aggtaaattt tccctgggcg caggcgtagg tgtcgttgag cacccatata aagattacga
     1921 taccgatgtt tacccagtac cggtaatcaa ctatgaaggc gataacttct ggttccgtgg
     1981 cttaggtggt ggttactacc tgtggaatga cgcaacggat aaactttcaa ttaccgctta
     2041 ctggtcgccg ctttacttca aagcgaaaga cagtggcgat caccaaatgc gtcacctgga
     2101 tgaccgtaag agcaccatga tggctggtct gtcttatgct cactttaccc agtacggtta
     2161 cctgcgtacc accctggctg gcgataccct ggataacagc aacggcattg tctgggatat
     2221 ggcctggttg tatcgttaca ccaacggtgg cctgaccgtg actccgggta ttggtgtgca
     2281 gtggaacagc gaaaaccaga acgaatacta ttatggcgta tcgcgcaaag agtccgctcg
     2341 cagcggtctg cgtggctata acccgaatga cagctggagc ccttaccttg agctgagcgc
     2401 cagctacaac ttcctcggcg actggagtgt ttacggtacc gcgcgctaca cccgtctgtc
     2461 tgatgaagtt actgacagcc cgatggtgga taaatcctgg actggcctga tttctaccgg
     2521 gatcacctac aaattctgat aatgcatctt tccagggcat tttgtgcata aaaacagggc
     2581 gtcacgccct gttttgcatt acggtggtgc aatacctggg aggaaacaat gcaacaaaaa
     2641 atgattcaat ttagtggcga tgtctcactg ccagccatag ggcagggaac atggtatatg
     2701 ggcgaagatg ccagtcagcg caaaacagaa gttgctgcac tacgcgcagg cattgaactc
     2761 ggtttaaccc tcattgatac cgccgaaatg tatgccgatg gcggtgccga aaaggtggtt
     2821 ggggaagcat taaccggtct gcgagagaag gtctttctcg tctctaaagt ctatccgtgg
     2881 aatgctggcg ggcaaaaagc gataaatgca tgcgaagcca gtttacgccg tctcaatact
     2941 gactatctcg atctctactt attacactgg tctggcagtt tcgcttttga agagactgtt
     3001 gccgcgatgg aaaaattgat cgcccaggga aaaatccgtc gctggggcgt ttctaacctt
     3061 ggttatgccg atatgcagga actctggcag ctgccggggg gaaatcagtg tgccactaat
     3121 caggtgcttt accatctcgg ttcacgaggg attgagtacg atctactccc ctggtgccag
     3181 caacagcaga tgccggtgat ggcttacagt ccattagccc aggccgggcg gttgcgcaat
     3241 ggactgttaa aaaacgcggt agtcaacgaa attgcacatg tccaacaaca tgcagctgcg
     3301 cttgaggtcg aactttcgtc agcggaatta gctatgctgg ataaggcata tccggcacca
     3361 aaaggaaaaa ctgcgctgga tatggtgtga tgttgatacg tgccggatgc atcgcacccg
     3421 gcacacaaca ttaacgcaaa ttaacgtttg gcaacgcgaa tggattgcgc cagatgtgca
     3481 ggtttctctt tggtgacttt ttgcgtttct gaagcgtaag ccgtttctac acaaacaaag
     3541 gtcttgtagc catcgtccgg catgtcgccc atgctaactg aaagcgccgg tcccgggttc
     3601 cagccgacaa cgttcagatg atgctggtgg cctacggcga taatacgatt cagcgcttca
     3661 tcattaatca cgctgcaatc ttgcggattc agatacacgc gatcggtacg gtcagggaag
     3721 gtctgaatac cgtcggtcag tacatcttct ttcgcgtcat tcactttatc aatgaagcga
     3781 tcgcccagcc cactgacgct taccttagcg atatcaccca cgttaaagta ggtatgcagg
     3841 gcagaggtgg tttcaaattc gccatgtgat tcaagatcga tttcacaggt tttacccacg
     3901 cggaaatgcg ctaacagcgt aaagtcgtgc ggccagaatt ttttcgtctc ttcgctttgt
     3961 gtcaattcaa aagtcagcgc tacgccatca gcatcttcat gatgtgattt cagcgtccac
     4021 ggcaggttgc gggcaaaacc gtgcgcaggc agaccttgtt gtgccgccgg accaaaccac
     4081 ggccagcaaa ccggtacgcc accgcgaata gcgacgccat ttttgaacgg tgtgttgttg
     4141 ctcaaccaca gaacttcttc ttcacccgca ggtttccacg agagaaggtg tgcgccctgt
     4201 aatgcaaaag aggcttttac ctggggatga tcgaccacaa tgaggtccag ttcatccagt
     4261 ttacgacggg agaggacagg ggagatttgt tcgatgaccg gaagggcaaa aattttctta
     4321 atcatgacgc agtcctttaa cttcatttta tcaggtaaaa aaaagagcga ccgaagtcgc
     4381 tctttttaga tcacagtgtc atctcaactt atttggagat gtgagcgatc aggtccagaa
     4441 ctttgttgga gtaaccggtt tcgttgtcgt accaggatac cagtttcacg aagttgtcgt
     4501 tcagagcgat accagcttta gcatcgaaca cggaagtgca aacttcgccg ttgaaatcgg
     4561 tagatactac gtcatcttcg gtgtagccca gaacgccttt catttcgcct tcagcagcag
     4621 ctttaacggc agctttgatc tgctcgtaag ttgcagcttt ttccagacga acggtcaggt
     4681 caactacgga tacgttcggg gtcggaacgc ggaacgccat accagtcagt ttgccattca
     4741 gttctggcag tactttacct acagctttag cagcaccggt agaggacggg atgatgttct
     4801 gggaagcgcc gcggccgccg cgccagtctt tgtgagacgg gccatcaacg gttttctgag
     4861 tagcggtagt agcgtgaacg gtggtcatca gaccttcgat gatgccgaag ttatcgttga
     4921 taactttagc cagcggagct aggcagttgg tggtgcagga agcgttggaa acgatgtcct
     4981 ggccagcata tttgtcgaag ttagcgcctt taacgaacat cggagtgttg tctttagacg
     5041 gaccagtcat aaccactttc ttcgcaccag cggtgatgtg tttacgagca gtttcgtcag
     5101 tcaggaacag accagttgct tcagcgacaa cgtcaacacc cacttcgtcc catttcaggt
     5161 tagccggatc acgttcagcg gtaacacgga tttttttacc gttaacgatc agatgaccgt
     5221 ctttcacttc aacggtaccg tcgaaacggc cgtgagtgga gtcatatttc agcatgtatg
     5281 ccatgtaatc agcgtctaac aggtcgttga ttgcaacgat ctcgatgtca gaacgtttct
     5341 gagcagcacg gaaaacaatg cgaccgatac ggccaaaacc gttgatacct actttgatag
     5401 tcatatattc caccagctat ttgttagtga ataaaaggtt gcctgtaaaa ttacaaaaac
     5461 cttacgcagc gtcaagcgga atcgtgtcaa tcattgcgac aaatcaatcc tgtgcctaag
     5521 cattacgcga ctgactcgcc tcacttttcc tttgggcttg agaccacatg gggtcggcgc
     5581 cccgaatttt aaagggcaat tacgataaaa atgtgatttt catcacgatt taggtgcagc
     5641 catttcgtcg agatcaagtt taggacaaaa ttttgccagt tggtgttaat gttttgttag
     5701 aatcagtcac gtaatgtgag cacgattaaa gtgagatgtg agtaaatggc gaataaacct
     5761 tcggcagaag aactgaaaaa aaatttgtcc gagatgcagt tttacgtgac gcagaatcat
     5821 gggacagaac cgccatttac gggtcgttta ctgcataaca agcgtgacgg cgtatatcac
     5881 tgtttgatct gcgatgcccc gctgtttcat tccgaaacca agtatgattc cggctgtggc
     5941 tggcccagtt tctacgaacc ggtgagtgaa gaatccattc gttatatcaa agacttgtca
     6001 catggaatgc agcgcataga aattcgttgc ggtaactgtg atgcccatct ggggcatgtc
     6061 tttcccgacg ggccgcaacc aacaggcgaa cgttattgtg ttaactctgc ttctttacgc
     6121 ttcactgatg gcgaaaacgg cgaagaaatc aacggttgaa aaaacgattc agcaacttat
     6181 tccacaggag cggaaaaatt atgaatcttg atgacattat caacagcatg acgcctgagg
     6241 tataccagcg tttgtcgacc gccgttgaac tggggaaatg gcctgatggc gttgcgttga
     6301 ccgaggaaca aaaagagaat tgcctgcaac tggtgatgct gtggcaagcc cgccataata
     6361 ccgaagcaca gcatatgaca attgacacaa atggtcagat ggtcatgaag agcaaacagc
     6421 agttaaaaga agattttggt atctcagcaa agcctattgc catgtttaag taagtggttc
     6481 atcccctttt tgccgcggct atgaagggga tagccaacaa tgaccccacg actcgtctca
     6541 attatctcga cccgacctgg ctaagtccca gtccgatctc ttgcagtcgc gttcacgcct
     6601 gaatgacttc tcctgaactt aagtctcaga cctatttggc cggtaatccc tctcgaattt
     6661 gctctttttg cgtcccgcct tccaccaacc tgtaacagac ccagccattt tctgcgtaat
     6721 tgtgatctga gttgtaaaat gcaggtcatt atctgtgttg tgcagattta atattgacct
     6781 caaaaatcat gtgggtaagt atgatggcca tctaagcaaa acaaaacaca gaggatgacg
     6841 caatgaaagc actggctcgg tttggcaagg cctttggcgg ctacaagatg attcatgtcc
     6901 cacaacccat ttgtggcccg gaagacgtag tgattgaaat taaagccgcg gcaatctgcg
     6961 gcgcagacat gaagcactac aatgtcgata gcggttctga tgagtttaac tctatccgcg
     7021 gccatgagtt cgcaggttgt attgcgcagg ttggtgaaaa agtcaaagac tggaaagtgg
     7081 ggcaacgcgt cgtgtcggat aacagcggtc acgtttgcgg cgtttgtccg gcctgtgaac
     7141 agggtgattt tctgtgttgt acagaaaaag taaaccttgg tctggataac aatacctggg
     7201 gcggtggttt ttccaaatat tgtctggttc ctggtgaaat tctcaaaatt catcgtcatg
     7261 cgttgtggga aatccctgat ggtgttgatt atgaggacgc agccgtactt gaccctatct
     7321 gtaatgccta taaatccatc gcgcagcaat cgaaattcct tcctggtcag gatgttgtcg
     7381 tcattggcac tggcccactc gggctgtttt ccgtacaaat ggcgcggatt atgggggcgg
     7441 taaatatcgt cgtcgttggt ctgcaagaag atgtggcggt ccgcttcccg gttgcaaaag
     7501 aactgggggc gacggcagta gtaaatggtt ctaccgaaga tatggtggcg cgctgccagc
     7561 aaatttgtgg caaagataat ctgggactgg tgattgaatg ctccggtgcc aatatcgcac
     7621 tgaaacaagc catcgaaatg cttcgcccga atggggaagt ggtacgcgtt ggaatgggct
     7681 tcaaacctct tgatttctcg attaatgaca ttaccgcctg gaataaaagc atcattgggc
     7741 atatggccta tgactccacc tcatggcgta acgctatccg gctattagcc agcggcgcta
     7801 tcaaagtcaa accgatgatc acgcatcgta tcggcctgtc gcaatggcgc gaagggtttg
     7861 atgcgatggt cgataaaacc gcaatcaaag tgatcatgac ttacgacttt gatgaataac
     7921 tactctcctg aaggaacgtt atcgcatgga acagataaca aaaccgcatt gtggtgcacg
     7981 gctggatcgc ttaccggatt gccgctggca ttcgtcaatg tttgccatcg tcgcgtttgg
     8041 cttgcttgtc tgctggagta atgccgttgg tggcttgatc ctcgcgcagc tgaaagcgtt
     8101 gggctggaca gataattcca ctacagccac attctcagca atcacgaccg ccggaatgtt
     8161 tctcggtgcg ctggtgggcg gcatcattgg cgacaaaacc ggtcgcagaa atgcgttcat
     8221 cctctatgag gccattcata ttgcctcgat ggttgtcggt gctttctcac cgaatatgga
     8281 ttttcttatt gcctgccgtt ttgtgatgga cgttgggctg ggagctttac tggttacact
     8341 gtttgctggt ttcaccgaat atatgcccgg tagaaatcgc ggaacgtggt ccagtcgggt
     8401 ttcctttatt ggcaactggt catatccgct ctgctcactg attgcgatgg gactcacgcc
     8461 gctgattagt gcagagtgga actggcgggt acaactgctt attcctgcaa tattgtcgct
     8521 tatcgctacg gcgctggcct ggcgctactt tcctgaatcc ccgcgctggc tggaatcgcg
     8581 cggacggtat caggaagccg agaaagtcat gcggagtata gaagaaggcg tcatacgcca
     8641 gacgggtaaa cctttgccgc ctgtggttat tgctgatgac ggtaaagcgc cacaagcggt
     8701 gccgtattca gccttactga caggagtatt actgaaacgc gtgatattag gctcttgtgt
     8761 gctgattacc atgaacgttg tgcagtacac actaattaac tggttgccaa caatattcat
     8821 gacccagggg attaatttaa aagactcgat tgttttaaat accatgagta tgtttggcgc
     8881 gccatttggt atttttattg ccatgctggt gatggataaa attccgcgta aaacaatggg
     8941 tgtggggcta ttaatcctga ttgcggtgct cggatatatc tattcactgc aaaccagtat
     9001 gttgctcata acgctgattg gtttcttcct gattactttc gtctatatgt acgtttgcta
     9061 tgcctcggca gtgtatgtcc ctgaaatctg gccgacagag gccaaactcc gtggctccgg
     9121 tctggcgaat gcggtagggc gaatcagtgg tattgccgca ccttatgccg ttgcagtgct
     9181 gctcagtagt tatggtgtaa cgggagtctt tattcttctg ggggctgttt caaatattgt
     9241 cgcaattgct atcgccacca ttggaattga aaccaaaggt gtctccgttg aaagtttaag
     9301 tattgatgca gtagccaata aataatacgg gtggattcaa aatgaaaaat tcaaaagcaa
     9361 tattgcaggt gccgggcaca atgaaaatta tttcagcaga aataccagtg cctaaagaag
     9421 atgaagtttt gattaaagta gaatatgtcg gtatttgtgg ttcagatgta catggttttg
     9481 aatcaggccc gtttattccg cctaaagacc caaatcaaga aattggcctg ggtcatgaat
     9541 gcgccgggac ggttgtggct gtgggaagcc gcgtgcgcaa atttaaaccg ggggatcggg
     9601 taaatatcga acctggcgtt ccttgcggtc actgtcgtta ctgtctggaa ggcaaatata
     9661 acatctgccc ggacgttgat tttatggcga cacaacccaa ctaccgcggc gcattaacgc
     9721 actatctgtg tcatccggag agctttactt acaaactgcc cgacaatatg gacacgatgg
     9781 aaggggcgct ggtggagcct gccgcagtcg ggatgcatgc cgcgatgctg gcagatgtta
     9841 aaccgggtaa gaagataatt attctgggag caggttgtat tggtttgatg acgttgcaag
     9901 cgtgcaaatg cctgggcgca acggaaattg ccgtcgttga tgtactggaa aaacgtctgg
     9961 caatggggga acagcctggt gcgacagtgg ttattaatgg cgcaaaagag gacactattg
    10021 cacgctgtca gcaatttacc gaagacatgg gcgcagatat tgttttcgaa acagcgggtt
    10081 ctgcggtcac cgttaaacag gcacctttaa tctggtaatg cgcggcggta aaattatgat
    10141 tgttggtact gtacccggcg attcggcaat caatttcctc aaaatcaatc gcgaagtcac
    10201 tatccagacg gtattccgct atgccaatcg ttatccggtc acgattgaag ctatttcttc
    10261 agggcgattc gatgtgaaat cgatggtgac gcatatttac gattatcggg atgtacaaca
    10321 ggcatttgaa gagtcagtta acaacaaacg cgacattatt aaaggcgtta ttaaaattag
    10381 cgattaatta atacgggaga acagaacatg ctcgcagata tcagatattg ggaaaacgat
    10441 gccacaaata aatattatgc aattgcccat tttaacgtat ggaatgcaga aatgttgatg
    10501 ggcgttaaag acgcagccga agaagcgaaa tccccggtta ttatttcttt tagtacaggt
    10561 tttgtcggta acacctcatt tgaagatttc tctcacatga tggtatcaat ggcacaaaaa
    10621 gcaacggtgc cggtaataac tcattgggat catggtcgga gtattgagat tattcataac
    10681 gcctggactc atggtatgaa ttcattaatg cgtgatgctt ctgcatttga tttcgaagaa
    10741 aatattcgtt taaccaaaga ggctgttgat ttctttcatc cgctgggtat tccggtagag
    10801 gcggaattag ggcatgtcgg taatgaaacc gtttatgagg aagcgctggc gggctatcac
    10861 tataccgatc ctgaccaggc ggctgaattt gttgaacgta cgggctgtga ctcattggct
    10921 gtcgccatcg gcaaccagca tggggtttat acgtcagagc cgcaattgaa ctttgaggtc
    10981 gtcaaacgcg tacgcgatgc tgtttctgtt ccgctggttt tgcatggcgc atcggggatc
    11041 agtgatgccg acattaaaac tgcaatttcg ttgggtattg cgaaaatcaa cattcatacg
    11101 gagctctgtc aggccgcaat ggtagcagtg aaagaaaacc aggatcagcc tttcctgcat
    11161 ttagaacgtg aagtacgtaa agctgtaaaa gaacgggcat tggaaaaaat tgaactgttc
    11221 ggttctgatg gcaaagcgga ataatgatat ggataatctc gacgttattt gtataggtgc
    11281 cgctattgtt gatattccat tacaaccggt cagtaaaaat atctttgatg tagattctta
    11341 tcctcttgaa agaatcgcaa tgagcaccgg gggcgatgca ataaatgaag caacaattat
    11401 ttctcgtctg gggcatcgta ccgcattaat gagccgcatt ggtaaagatg cctcagggca
    11461 atttattctc gaccattgtc gcaaagagaa tattgatatt caaagcctga aacaggacgt
    11521 cagcatagat acatctatta acgttggact ggtgacggag gatggcgagc ggacgtttgt
    11581 cacgaaccgt aacggcagtt tgtggaaatt aaatattgac gatgttgatt ttgcgcggtt
    11641 tcctcaggcg aaattattat cactggccag tattttcaac agtcctctat tggatggtaa
    11701 agcgctaaca gaaattttta cgcaagcgaa agcccggcag atgatcattt gtgccgatat
    11761 gatcaaaccg cggttgaatg aaacgctgga tgatatttgc gaagcattga gttatgtcga
    11821 ttatctgttt ccaaattttg ccgaggaaaa attactcaca gggaaagaga cactggatga
    11881 aattgctgac tgctttcttg cgtgcggcgt aaaaacggtg gtgattaaaa cgggtaaaga
    11941 cggctgcttt atcaagcgtg gtgacatgac gatgaaggtg ccggcggtcg caggaataac
    12001 cgccatcgac accattggcg cgggcgataa ctttgcttca ggttttattg cggcactgtt
    12061 agaaggcaaa aatctgcgtg aatgcgcacg ctttgccaat gcaacggcgg ctatctcggt
    12121 tctaagcgtc ggtgccacca ccggcgtaaa aaacagaaag ctggtcgaac aattgctgga
    12181 agaatacgaa ggataatgaa ggcaaatgaa aaagatacct ttaggcacga cggatataac
    12241 gctttcgcga atggggttgg ggacatgggc cattggcggc ggtcctgcat ggaatggcga
    12301 tctcgatcgg caaatatgta ttgatacgat tcttgaagcc catcgttgtg gcattaatct
    12361 gattgatact gcgctaggat ataactttgg caatagtgaa gttatcgtcg gtcaggcgtt
    12421 aaaaaaactg ccccgtgaac aggttgtagt agaaaccaaa tgcggcattg tctgggaacg
    12481 aaaaggaagt ttattcaaca aagttggcga tcggcagttg tataaaaacc tttccccgga
    12541 atctatccgc gaagaggtag aagccagctt gcaacgtctg ggtattgatt acatcgatat
    12601 ctatatgacg cactggcagt cggtgccgcc attttttacg ccgatagctg aaactgtcgc
    12661 agtgcttaat gagttaaagg ctgaagggaa aattcgcgct ataggcgctg ctaacgtcga
    12721 tgctgaccat atccgcgagt atctgcaata tggtgaactg gatattattc aggcgaaata
    12781 cagtattctc gaccgggcaa tggaaaacga actgctgccg ctatgtcgtg ataatggcat
    12841 tgtggttcag gtttattccc cgctagagca gggattgttg accggcacca tcactcgtga
    12901 ttacgttccg ggcggcgctc gggcaaataa agtctggttc cagcgtgaaa acatgctgaa
    12961 agtgattgat atgcttgaac agtggcagcc actttgtgct cgttatcagt gcacaattcc
    13021 cactctggca ctggcgtgga tattaaaaca gagtgattta atctccattc ttagtggggc
    13081 tactgcaccg gaacaggtac gcgaaaatgt cgcggcactg aatatcaact tatcggatgc
    13141 agacgcaaca ttgatgaggg aaatggcaga ggccctggag cgttaaatac tcaacacgat
    13201 tcacctctgt ttctgtgaaa actgtgatga aaatctgtga ttcaaatagg ttattttgaa
    13261 agtaaacatc ggttatgcga taatcgcgct aatgtgatgt aaaaagatac aggggtgtaa
    13321 tcgtggcggc aaaagacagg attcaggcaa ttaagcaaat ggttgccaac gataaaaagg
    13381 tgacagtctc aaatttgagt gggatttttc aggtaaccga agaaaccatt cgccgcgatc
    13441 ttgagaagct ggaagatgaa ggctttttga ccagaaccta tggtggtgct gttttaaata
    13501 cagcggtgtt gacggagaat atccattttt ataagcgcgc ttcatcgttt tatgaagaga
    13561 agctgcttat tgcacgcaag gcactaccct ttatcgacaa taaaaccacc atggcagccg
    13621 attccagtag taccgtaatg gaattgctca aattgttaca ggaccgtagt gacctgacgt
    13681 tgctaaccaa ctcggcggaa gcgattcatg ttctggctca gtcagaaatt aaagtcgttt
    13741 caacgggtgg ggaactaaac aaaaatacac tttccctgca aggaagaatt actaaagaga
    13801 tcatcagccg ctaccatgtc gatatcatgg taatgagttg taaaggtctt gatattaaca
    13861 gcggcgcgct ggactctaat gaagcggaag ctgaaatcaa aaagacaatg atccgtcagg
    13921 cgacagaagt tgcgttattg gttgatcact ctaagtttga tcgtaaagct tttgtccagt
    13981 tagctgactt tagtcatatt aattatataa taactgataa atcaccgggt gcagagtgga
    14041 tagcattttg caaagacaat aatatccagc tggtgtggta atttaagcac acgttgtcaa
    14101 tattatttgt tcctgcgact ggaaataaaa tgatgataaa gatgcggcaa gtgaatttgc
    14161 cgcaattgct accttttata gcaaatccga ggcacccaat ggaacaatat gatcaaattg
    14221 gcgcaagact ggaccgcttg cctttggccc ggtttcatta tcgtatattt ggtataataa
    14281 gctttagtct gttattaacg gggtttttga gttactcagg taatgtcgtc ttagcaaagc
    14341 tggtaagcaa tggatggtca aataatttcc tctatgccgc ctttacctcg gcattaatgt
    14401 ttggttattt catcggctca cttactggtg ggtttattgg tgactacttt gggcggcgca
    14461 gggcgtttcg cataaatctt ctcatcgtcg gtattgctgc aacaggggcc gcttttgtcc
    14521 ctgatatgta ctggctcatt ttctttcgct tcctgatggg aacaggaatg ggggcgctga
    14581 ttatggttgg ctatgcctca tttacggagt ttatccccgc gacggtgcgt ggaaaatggt
    14641 ccgcgcggct ctcatttgtt ggtaactggt cgcccatgct gtctgcggcg ataggcgtgg
    14701 tggttatcgc tttttttagt tggcgaataa tgtttcttct gggtggtatt ggcatactgt
    14761 tagcctggtt tctctcaggt aaatacttta tcgagtcgcc acgatggctg gaagggaaag
    14821 ggcaaatcgc cggtgcagaa agccaacttc gtgaagtaga gcagcaaatt gaaagggaga
    14881 agagtattcg tttaccccag cttactttga accagagcaa cagcaaggtt aaggtaatca
    14941 agggtacctt caggctcctg tttaaagggg aaatgttacg acgtacatta gttgcgataa
    15001 ctgttttaat tgcaatgaac atttcgcttt ataccattac cgtatggata ccgaccatat
    15061 ttgttaactc cggcattgat gtcgataaat caatattaat gaccgctgtt attatgattg
    15121 gtgctccggt aggaatattt attgcagcat taattattga tcattttcct cgtcgattat
    15181 ttggctccgc cttacttatt attattgccg tgttaggcta tatctattca agtcagacta
    15241 cagagtgggc gattttaatc tatggtctgg tgatgatctt ctttttatac atgtatgttt
    15301 gcttcgcgtc ggcggtttat atcccggagc tttggccaac gcatttacgc ctgcgcggtt
    15361 cgggtttcgt taatgccgtc ggacggatcg tcgcagtttt cacgccctat ggcgttgcgg
    15421 cattattaac acattatggg tcgatcacgg tgtttatggt gcttggtgtc atgttagtgc
    15481 tctgtgcgct ggttctctcc atttttggca tcgaaacgcg gaaggtgtcg ctggaagaga
    15541 tttctgaggt gaattaaatc tttcgttgat ggcatcaatt gccagatgta ccactgacgt
    15601 gttttatttg gcctacaatg ccagtacaga attgtaggcc agcgtaaaat tacccctgcg
    15661 tctcttccca gtctgccagc gtatatagcg ttgccccagc tgctgacatc tccataaacg
    15721 cgtgtgcact gtcctggggc tggatattca cgccacgaca gccatcggta atcacgttta
    15781 ccttataacc taactgtaac gcgtccagca cggtaaactt cacgcaatag tcagtagcca
    15841 ggcccataac gatcaattca tcgatttcat gatcgcgcaa ccagtcatca agcgcggttt
    15901 tctgccgacg tccgttatca aaaaaggcac tgtaactgtc aactaaagga ttttcgcctt
    15961 tatggaacac cgctgcgatc tctttttgat tcagtaacgg atgtaattgt gcgccttcac
    16021 tgttctgcac acagtgttca ggccagaaag tttgccgcag gccgccgagt tggcctggcg
    16081 tataaggctc tacgccgtgc tggctggcaa agctgccgtg attcgccgga tgccagtcct
    16141 ggctggcgat aaccgcttct ccgcgcgact ggcaccagtc aataaggcgg ttagcaacct
    16201 ccaccgtact gtcaccttcc ggtacggcga gcgcgccacc agcacagaaa tcattttgta
    16261 aatcgaccag taacagggcg cgatggggca ttgcagtctc cttaatcatc cggcgtcagt
    16321 tcgccgcgca ggttttggct catggccttg cgaatggttt cagtatccag ttcctggctc
    16381 agcaggtaat gcagtttggt tagcgtggct tctacagtca tatctgcacc gtcaataacg
    16441 ccggcatggg cgagggcgtt accggtggca taaccactca tattcacttt accggacata
    16501 cattgtgtca ggttgaccac cacaataccg cgatcgctgg cttcttgtaa ttcctgcagg
    16561 aaggctttgt tttgtggcgc attacccacg ccataggagc gcagaatcaa tgctttcacc
    16621 ggttggcgca gaaaattgcg cacgacgtca gcagaaatcc ctggataaat cgtcaccacg
    16681 ccaattggtt gtggcgtgat tggatgaacg attaattccc cttcaccgtg cggggcgggt
    16741 ggcgtattca aacgacgaat atggatacca gcttccagta acggaggaag gtttggagag
    16801 gcaaacgcat caaaaccatc ggcatgggct ttggtagtgc ggttgccgcg atacaatcgg
    16861 ttattgaaaa agagcgttac ttcgttgatc ggataattcg ccgcaacgta caacgcattc
    16921 agcagattaa tttgtccgtc agagcgtaac tcagccagcg ggatttgtga ccctgtcaca
    16981 atgaccggtt taccgagatt ctcgagcatg aacgacagcg cagaggcggt atacgccatc
    17041 gtgtcggtac cgtgtagaat gacaaaacca tcatagtcgt catagtgcgc tttaatatct
    17101 tcagcaatat gctgccagtc ttccggcgtc atatcagaag aatccatcaa cggcgtatat
    17161 tcatgaatgg tgaaatctgg catctccggg cgatggaatt ccggcatcag cgccagttgg
    17221 cgttgtagat gacctgacac cggaatataa ccctgctcgg aacgctgcat cccgatggtc
    17281 ccgcccgtgt aggcgacgta aattgatttc ttttgcatga tattgatatg aatgatatcg
    17341 aaagagcaaa agtatatacg tgaggcagga ggagcgcacc tgatactatg cggataagga
    17401 aatatggggc gagacagcgg gaagaggatt cgtcgaggta aaaaataatg tggccgacga
    17461 tctgtcggcc actcagtaca agacttaacg cacgttggcg caggtcaggc aaaacgcata
    17521 acggttttgt gggtcgttaa acgcggccag cttgtcgctt tcacttttaa cggtagaggc
    17581 taccgaggca agcggtgcag gtaacatggc ctggaacgca tctggcaaca ttgcccggac
    17641 agaaccagac atgttgtcca tcactttgtc gaagaaggtc ggttcatcaa cgtagtattc
    17701 cagatgccac tgtttcactt ttgcgagctc tgctgctttg gcaaccgcat catcgaaatc
    17761 cccgagacta tcgaccagcc cgttagcttt tgcatcctga ccggtccaga cgtggccctg
    17821 ggcgatttta tcaatttgct ccggcgtcga atgacgcgca tcagcaacca gcgtgataaa
    17881 gcgtttatag ccattctcaa tgcttaattg catcatctgc tgcgcttccg gcggcagtgc
    17941 cctggtgata gaaacatccg ccagcggtga agttgagaca ccatcagtat gaacaccaat
    18001 cgaatccaga ctattttcta cggtggtgat cacgccgaag ataccgatag aaccggtcag
    18061 ggtgctgggg ttagccacaa tgtaattagc tggcgtggaa atccagtaac caccagatgc
    18121 cgccatgccg cccatcgata caaccacaga cttacccgct gcccgggctg ctgccagttc
    18181 agcgcgaatc acttcagacg cggtaacgct gccgcctggg ctattaacac gcaggacaat
    18241 cgctttcact ttcgggtcaa ggcgagcgtc gcggatttgt gccgcagtgg tatcaccgcc
    18301 aacattcccc tgagtttcct cgccatccat aattgcgcca ttagcgaaga cgacaccgat
    18361 gctgtcaccg gtatctgccg gcgttttcaa tgcgtaatcg taataactga tggcgcgata
    18421 atttttatca gtcttactcc agccgaactc tttggtcaga gctttttcga tttcggcact
    18481 cgatgccagt gcatcgacca gcttgttttc cagtgcatat ttcgcggtat cgccaccggt
    18541 tttggttaaa ccctcaagca acccttgcgc gccagggaat acctgctgag cagggatctg
    18601 ccggttagcg gcaacagtat tcagatagtt ttgccacagc tcaccaatcc agcggctgtc
    18661 agcttcgcgg gctgccggtg acatatcatc acgaataaac ggttcaacgg cagatttata
    18721 cgtacccacg cggaacacat gggtggaaac tttcagctta tccagcaacg atttgtagta
    18781 cagaccgttg gtagcaaaac cgtgcagatc aaccacgcct tgcggtgaca gccaaatttt
    18841 attggcgaaa ctggcgagat aatattgccc ctggctgtag ttctcgccaa cggcataaac
    18901 cggtttcccg ctgtcacgaa actctttcag ggctttgccg atgtactgca tagacggctg
    18961 gtcgccgcct gcgaagtttt tcagatccat cacgataccg gtgatattgc ggtcgtcctt
    19021 cgcctggcga atggtgttga cgatatcaaa cagtgagttt tcctgcagac gatcggaact
    19081 ggcaccaagc agctggcggc ttaatttact aaaccgctga gaactgtctg gtttatcgac
    19141 gatcacacca gaaatgtcca gcagcagtgc gccacgactg gccgtttctt tcgaatcacc
    19201 accactgacc tgcatccaga tccccacacc aaccagtacg aggaaaataa agaacaggtt
    19261 aagtaccatt tcacggacga aattcagcag acgccacgtc catttaaaaa atccggcaat
    19321 aaatcgccaa agggttcgca tgtattctcc caacttaagg tcacacctgt cacaggcgca
    19381 atagatgtgg ttatcgtaat gacccaaccg gcacttgtca gcaggaattg cggactgtgc
    19441 tgtaacaaaa tccactctcg tgttaatttt gtgaataaat atcacgacag gagttaatca
    19501 aatggatgca ctcgaactat tgatcaatcg ccgtagcgcc tcccgcctgg ctgaacccgc
    19561 gccaacgggt gaacaactgc aaaacatcct gcgtgcgggt atgcgtgcgc cggaccataa
    19621 gtccatgcaa ccgtggcatt tttttgtgat tgaaggggaa gggtgcgagc gtttcagcgc
    19681 cgtactggaa cagggggcga ttgctgccgg tagtgatgat aaagctatcg acaaagcccg
    19741 taatgcgccg ttccgcgcac cgctcatcat cacggtggtg gcgaaatgca aagaaaatca
    19801 taaagtcccg cgatgggaac aggaaatgtc tgccggatgc gcggtcatgg cgatgcaaat
    19861 ggcagccgtt gcccagggat ttggcggcat ctggcgcagt ggcgcattaa ctgaaagtcc
    19921 ggtagtgcgt gaagcattcg gttgccgtga gcaggataaa attgtcggtt ttctctacct
    19981 cggcacgccg cagttgaaag catctacgtc gattaacgtc ccggacccga cgccgtttgt
    20041 aacttatttc tgataattgt cgcgaaactg tctggattgt gagcaaaggc gcaggaattc
    20101 agacactctc acttatcact tcacggaatg agggttacca tacccggatt gcattgacag
    20161 gagatgtcca tgagcgagaa ctcgattcgt ttgacccaat acagccacgg agctggttgc
    20221 ggctgtaaaa tttccccaaa agtgttggaa accatcctgc acagtgagca ggcgaagttt
    20281 gttgatctga atttgcttgt gggtaatgaa acccgcgacg atgcggcggt gtacgatctg
    20341 ggcaatggca ccagcgttat cagtaccacc gacttcttta tgccgatcgt tgataatcct
    20401 ttcgattttg gccgcattgc ggcgactaac gccatcagcg atatcttcgc gatggggggc
    20461 aaaccgatta tggcgattgc gatcctcggc tggccgatta acaaactttc cccggaaatt
    20521 gcccgcgaag taaccgaagg tggacgctat gcatgccgtc aggcgggtat tgcgctggct
    20581 ggcggtcact ccatcgatgc gccggagccg atttttggtc tggcggtaac ggggatcgta
    20641 ccgaccgagc gggtgaagaa aaacagtacc gcacaagccg gatgcaaact gttcctgacg
    20701 aaaccgctgg ggatcggcgt tcttaccacg gctgagaaaa aatcactgtt gaaaccagaa
    20761 catcagggac tggcgacgga agtgatgtgc cggatgaaca tcgcaggcgc gtcctttgcc
    20821 aacatcgaag gcgtaaaagc gatgaccgac gttacgggct ttggtctgct gggccacttg
    20881 agcgaaatgt gtcagggggc tggtgtgcag gcacgcgtcg actatgaagc gatcccgaaa
    20941 ctccccggtg ttgaagagta cattaagttg ggcgcagtac ctggcggcac tgaacgtaac
    21001 tttgccagct acggtcatct gatgggtgaa atgccgcgtg aagtgcgcga tctgctgtgc
    21061 gatccgcaaa cttctggcgg tttgctgctg gcggtcatgc cggaagcaga aaatgaggtt
    21121 aaaactacag ccgccgagtt tggcattgaa ctgacggcga ttggcgaact ggtgccagcg
    21181 cgcggcggtc gtgccatggt tgagatccgt taatttaatg cggttgttta ttgccgaaaa
    21241 accgagtctg gcgcgcgcca ttgctgatgt cctgcccaaa ccgcaccgga aaggcgatgg
    21301 ctttatcgag tgcggtaatg gtcaggtggt gacttggtgt atcggtcacc tgcttgagca
    21361 ggcgcagcca gacgcctacg acagccgcta tgcgcgctgg aatcttgcgg atttgccgat
    21421 tgtcccggaa aagtggcaat tacagccacg accctccgtg accaaacaac ttaacgtcat
    21481 caaacgcttc ctgcatgaag ccagcgaaat cgttcacgcc ggggacccgg atcgtgaagg
    21541 gcaattgctg gtggatgaag tgctggacta tctgcaactg gcaccggaaa agcgccagca
    21601 ggtgcagcgt tgcttgataa acgacctgaa cccgcaggcg gttgagcggg cgatcgaccg
    21661 tcttcgttcc aacagtgagt ttgtgccatt gtgcgtttct gcgctggcgc gagcgcgtgc
    21721 cgactggctg tacggcatca atatgacccg tgcgtatacc attctcggtc gcaatgccgg
    21781 ttatcagggc gtactttccg tgggacgcgt gcagacaccc gtgctcgggc tggtggtgcg
    21841 ccgcgatgaa gagattgaaa acttcgtggc gaaagacttc tttgaagtca aagcacatat
    21901 cgtgacacct gccgatgagc ggtttaccgc tatctggcaa ccgagcgaag cgtgtgaacc
    21961 gtaccaggat gaagaagggc gcttgttaca tcgtccactg gcggagcatg tggttaaccg
    22021 cattagtggt caaccggcta ttgtcaccag ctataacgat aaacgggaat cagaatccgc
    22081 gccgctgccg ttttcgcttt cggcgttgca gattgaagcg gcaaaacgct tcggtctgag
    22141 cgcgcagaac gtgcttgata tctgccagaa gctgtacgaa acgcacaagc taatcactta
    22201 tccgcgttcc gattgtcgct atttgccaga agaacatttt gccggacgcc acgcggtgat
    22261 gaatgccatc agcgttcatg caccggatct gttgccgcag ccagtggtag atccagatat
    22321 acgcaaccgc tgttgggatg acaaaaaggt cgatgcgcac cacgccatca tcccgaccgc
    22381 acggagttct gcgatcaacc tgacggagaa cgaagcgaag gtctataacc tgattgcccg
    22441 tcaatacctg atgcagttct gcccggatgc ggtgttccgc aagtgtgtta tcgaactgga
    22501 cattgccaaa ggcaaatttg tcgctaaagc ccgttttctt gctgaagcag gctggcgcac
    22561 gctgttaggc agcaaagagc gcgatgaaga aaacgacggc acgccattgc ctgtggtggc
    22621 gaaaggcgat gagttgctgt gtgaaaaagg tgaagtggta gagcggcaaa cccagccgcc
    22681 gcgccatttt accgatgcaa cactgctttc ggcgatgacc gggatcgcgc gctttgtgca
    22741 ggacaaagat ctgaaaaagg tccttcgtgc gaccgatggt ctggggacag aagcaacccg
    22801 tgccgggatt attgaactgc tgttcaagcg tggttttctg accaaaaaag ggcgctatat
    22861 ccactccacc gacgccggaa aagcgttatt ccattcgctg ccagaaatgg cgacgcgacc
    22921 ggacatgacc gcgcactggg aatcggtgct gacgcaaatc agcgaaaagc agtgtcgcta
    22981 tcaggacttt atgcagccgc tggtggggac gctatatcag cttattgatc aagccaaacg
    23041 tacgccggtg cggcagtttc gcggcattgt ggctccgggc agtggtggca gtgctgataa
    23101 gaaaaaggct gcaccgcgta aacgtagtgc gaaaaaaagt ccgccagcag atgaagccgg
    23161 aagcggggcg atagcgtaag cgagcgaatc tttcgtgcta ttcgagtcat attctgaaat
    23221 atccagcgga tcaagaaaat tcgttggata tttttttgca tggataaaat tatcgcctct
    23281 aaagtatgta ataacaggga atgtgatgaa aaaagtttta ttgcaaaacc atcctgggag
    23341 cgagaagtat tcttttaatg gctggggaat atttaatagt aattttgaac gggtgattaa
    23401 agaaaataag gccatgctgc tttgtaagtg ggggttttat ttaacatgtg ttgtcgctgt
    23461 aatgtttgta ttcgcagcga taacatccaa cggtttgaat gaaagaggcc tgattaccgc
    23521 gggatgctct tttctttatc tattaattat gatggggctt attgttcggg ccggttttaa
    23581 agcaaaaaaa gaacaactgc attattatca ggctaaaggt attgagccgc tcagtatcga
    23641 gaagttacag gcgctacaat tgatcgcacc ttatcgattc tatcataagc aatggtctga
    23701 aacgctagag ttctggccgc gaaagcctga acctggcaaa gataccttcc aatatcatgt
    23761 gcttcctttt gactcgatcg atatcatcag taaaagacgc gagtctttag agaatcaatg
    23821 gggtatcgaa gatagcgaaa gttattgtgc cttaatggag cattttcttt ctggcgacca
    23881 tggagccaat acctttaaag caaacatgga ggaagcccca gagcaggtta tcgccttgtt
    23941 gaataaattt gctgtttttc cctcagacta tatctctgat tgcgctaata atcgctccgg
    24001 taaatcctcg gcgaagctaa tatgggcggc ggaattatca tggatgatct cgatatcaag
    24061 cacagctttt caaaacggga caattgaaga agaactggcc tggcattata taatgcttgc
    24121 ttctcgaaag gcgcacgagt tgttcgaaag cgaagaagat tatcaaaaaa atagtctaat
    24181 gggatttctt tactggcata tctgctgcta tcgcagaaag ttaacggatg ctgaacttga
    24241 agcgtgttat cgttacgaca agcagttttg ggagcactac agtaagaaat gccgttggcc
    24301 cataagaaat gttccctggg gagcatcatc cgttaaatac tcataaacgc ctgaaatttt
    24361 gccgggggcg ctgcgcttga ccggcctaca ttagagcgta actgcaatga attgtgccca
    24421 tctgtaggcc tgataagcgt agcgccatca ggcatttaca acttaaatca caccctgcgc
    24481 cagcatcgca tcggcaacct tcacaaaacc ggcaatgttc gcgccctgca cgtagttggt
    24541 ttgctcacct tcaccaccat gctcaacaca ggcatggtgg atatccagca tgatgtgatg
    24601 caaacgtgcg tcaactttct cggctttcca gcccaggcgc gcagcgtttt gtgccatttc
    24661 caggcccgac gttgctacac cgccagcatt agccgcttta cccggtgcaa acagtacgcc
    24721 tgcctgctgg aacagttcag tcgcttcgat ggtggtcggc atatttgccc cttcagcgac
    24781 ggctttaacg ccattggcga taagctgatg cgcggcgtca acatccagtt cgttctgggt
    24841 ggcgcaaggc agggcgatat caaccggtac agaccacggc tgttggcctt cgagatagac
    24901 cagaccaaat tctttggcgt aatctgccac tcgaccatcg cggctggctt tgatttcgat
    24961 aagacgtgcc agtttctctt tcgtgaatcc gctttcatca actacagtgc cgctggagtc
    25021 tgacgcagtg atcacacgag caccaaattc catcgctttt tcgatagcgt actgggcgac
    25081 gttgccggag ccagaaacgg aaacgctcat cccttcaaaa cccataccgt ggcgttttaa
    25141 cattgcttct gtgaaataaa ccagaccgta gccggtagct tccgggcgaa taagactacc
    25201 gccaaatgaa aggcccttac cggtgaagac gcaggcggta ttgttggaga gctttttcat
    25261 catccccgcc ataaagccga cttcacgacc accaacgccg atatcacctg ccggaacgtc
    25321 ggtatccgcg cccaggtggc gatacagttc agtcatcagc gtctggcaaa aacgcatcac
    25381 tttaccttcg ctttttcctt tcggatcgaa atcgctgccg cctttaccac cgcccatcgg
    25441 cagggtagtc agggcatttt tgaaggtttg ttcaaagccg aggaatttga gaatggaaag
    25501 gttaactgac ggatggaagc gcataccgcc tttgtacggg ccgatggcag agctgaactg
    25561 cacacgccat gcacggttga cctgtatctg gttgcgatca tcaacccata ccacgcgaaa
    25621 ctggatcacg cgctccggtt caaccagacg ctccagtaat gacatctggc gatattttgg
    25681 attttgttca agaaaaggcc agagtgtggt cattacttca cgaacggctt gcgcgaactc
    25741 ggtttgattc gggtcgcgct tttggacatg gttgaggaat gactccagag aatatgtctg
    25801 atccatagat ataaaaacct tatatattaa tacgattgtg cttatgttgt ttatgtcatg
    25861 tgcttttgca gttttcgact ataccatcaa gaatgtgacg gtaagcaaga aaaaaatgac
    25921 ccaggaaagc aaattaataa cgagagtaat ctcataacca aatgcgataa ctcagacgct
    25981 aaagttttgc cacccggcta ccgttatcag tagcagagac aataacgtta aggagttccc
    26041 tgtgagtcga gcattgttcg ccgtagtatt agcatttcca ttaattgcac tggctaaccc
    26101 gcattatcgt ccggacgtgg aggtaaacgt accgccagaa gtgttcagtt caggcgggca
    26161 gtcagcgcag ccctgtactc agtgctgcgt ttatcaggat caaaattatt ctgaaggggc
    26221 ggtaattaaa gccgaaggaa tactgctgca atgccagcgt gatgacaaaa cccttagcac
    26281 taatccgctg ctctggcggc gcgtaaagcc ataaacgcct ctaataatgg aatgtcagca
    26341 ggggccagcg gatattgcag cgcctcttca ggtgagcacc agaccagcgc ctgatgttca
    26401 tgtgcctgta acgtcccgtg gaagtcgggt acgtgccagg catgaagatg gataatccgg
    26461 cccgaaactt ctcgctgatg gctggcaaca tactcaccca cagttgcttc gatgtccaat
    26521 tcttcgttta actcacgcac cagcacctgc tgctggcttt catccagctc gactttacct
    26581 ccggcaaact cccataatcc cgcctgatcg ctctgggcgg ggcgttgcgc gagtaaaatt
    26641 ttgccatcac gttcaatgat ggcggcaaca acttcaatca ttttcataaa ttacacacgg
    26701 gcagataagc gaaaaatgcc ataatatccg cactgttgct caatcagaaa ggctttatat
    26761 ttaaggagtg cctgtgctag accgccatct tcatccccgg attaaaccgt tgttgcatca
    26821 gtgcgtgcgg gttcttgata aaccgggcat tacgccagat ggtttaacat tagtgggatt
    26881 tgccatcggc gtgctggctc tgccgtttct ggcgctgggc tggtatctgg cggcgctagt
    26941 cgtcattttg ttgaacaggc tgcttgatgg tctggacggt gcgctggcgc ggcgcagagg
    27001 gcttaccgac gcgggcggct ttcttgatat ttctctcgat tttctctttt acgcgctggt
    27061 gccgtttggc tttattcttg ctgcaccgga gcaaaatgcg ctggcaggcg gctggttgtt
    27121 gtttgcgttt atcggcacgg gcagcagttt tctcgccttt gctgcactgg cagcaaaaca
    27181 tcagattgat aaccccggtt atgcacataa gtcgttttac tatctggggg gattaaccga
    27241 aggcacagaa acgatcttac tgtttgtgct gggatgttta ttccctgcat ggtttgcgtg
    27301 gtttgcgtgg atcttcggtg cgttgtgctg gatgacgacc tttacgcggg tgtggagtgg
    27361 gtatctgacg ctgaagtcgc tccagcgtca gtaattgcaa tgttatttgc tactgcccgg
    27421 gccgcgttca ccggttgcaa ccggattttt tggatcgctg ctccattcgt accagccgcc
    27481 gtcatacacg gaaacattct tccaacccat ggcgcgtgcg tacataaagg tttcggacgc
    27541 gcgccagccg gtgccgcagt agaatgaaac ttgctgatct ggtttgatat tccatgcttt
    27601 ccacatagcg gtaatatcat cggcgctacg catggtgcca tccgggttat agaaatcttc
    27661 catatgcgtc gagtcgctac cagcgtgtcc ccaacgtgct ccggctattt caccttttgg
    27721 tttaatatag ctgtaaccgc tggtcgtacc gataaattct ggccacgaac gaatgctcac
    27781 cagcgatgca tcctggcgat gcagcagtcc acgggcctgt tccatatcga gcatcaactg
    27841 cggttgtgcc gggatcttca cgccgaaatc cggttccgct ttcactttcg gtggcgttcc
    27901 tcgctcaaca ggcagtcccg cgtcggacca ggtttgccag ccgccatcca gcagacgcac
    27961 atctttcacg cctgcgtaga gcataatttg cgccacacgc gctccagcgt atacgtcacg
    28021 cccgtacaga atgaccgtgg tgtcatggcg aatgccgtgt tttgccagca tcgcttttag
    28081 ttgttcatca gaaactttgt tccacagcgg ttcgctttcc acttcgttgg tatcgatgta
    28141 gtcagcgcca ggaatatggc tgataaggta aagcttagga gcgccctagg ccgcttcaat
    28201 gactttccag tcaccgacag gtttcgccgt aacctcttta ccttgttgca ggtcatgcag
    28261 ccattgcgga taaaccagct gctcaaagtg cggcagtttt tgtagacggg aaggttcgct
    28321 tagcgcgtca ctcagaatgg agatatgcgt aaaacctgct ttttgcagtc gcgttttgac
    28381 ggcgtcgaca tctttgccat taccgtacag cgccaccggg gcatcggctt tcaggttatg
    28441 ttgcttgatc cacgcgttga gctgttcggt gctcattttg tcgagccagc tagcagagag
    28501 gtttaaggcg gcaggttcat gaccagaagg gccatttaag gtttgtggcc agccgttata
    28561 aaacgcgctg gggcgagtat ctatcgcttt gccattttgt tgctgaagtt ggtcaagtgt
    28621 aagaggcttc gccagttcag cggcccacga agaagcgcaa gccagcccta aagccattgc
    28681 cagcgcggtc atttgagaaa cacgtttcat cgaaaatccc gacattgtag ttttgagtgc
    28741 gccattctga actgacgcat tgagaaaaac tttgcgttat cgcagtttgt tgtaattttc
    28801 tgaccactgc gccatatcca gaacagaact atcagcagga acatcctgga gatcgtgcgt
    28861 tacctgaacg acggggatcg ccagggcgcg aacttcgctg aacacccact ggcgaaaatt
    28921 atcgcgcaga gccacatcaa gacggctgaa tggctcatcc aggagtaacg cttttggctg
    28981 ggcgagaagg gcgcgtagta gagcaacgcg cgctcgctga ccgccagaca aagtggcagg
    29041 atcctgatgg aaagctccgt caaggcctga acgctcaagt gcatcattca cggcattacg
    29101 tcgggcattc cccttaagtg tcgccggtag cgccagcagt aaattttgcc cgacgctgaa
    29161 ctggtcaaat aacagtgcat cctgaaaaag aatgccaatc tgacgctgtg cggtgggtag
    29221 catgtcaatc cgttgctcat tgagccatag ctcacctgta caagaaaact gtccggccag
    29281 tgcaccaatc atccatgaaa acagagtgga ttttccacag ccagacggcc ccattaacgt
    29341 gacgatgtca cctttatcaa ccgtaaagtt aacgtttgtc agcaagcggc tttctggtaa
    29401 acgtagcgaa acatttttca cgcagagcat tagcggattc cttgtctgac ataacctacc
    29461 cattttgcga ctaacgcggt cagggcaaaa ataataagcg gtaacaacag ttgccataac
    29521 gcctgggcgg cgagaatacc gttgctgccg ccgctgctta atgccaccgc ctcagtggtg
    29581 agcgtcggaa aacgccccgc gcccagccac agcgtcggca tatactgcgc aatactgact
    29641 gaaaatccca ccgcgaaggc agtcagcaca gggcgcaaca tgagtgggca tttcacgtaa
    29701 aagaagattt tggcccgcga ccagcccagt gtttgcgcaa tcaaaattaa ccgtgaatca
    29761 atgcgctgcc aggcgggttg caggataaac agcatccacg gcattaccca cagcagatgc
    29821 ccccagacca ccgccgtcca gcttccatcc aggttcagcc atagcgccag cgtgtactgg
    29881 cccgccacca gtggcagagc gggcaataga atgggcaacc atagccacaa ctggcgacgc
    29941 tgtgcccccc attccagcca cagcagtaac aggagcaaag cgatgaatgt tgctaccagc
    30001 cccattgtca gactgttgat gagtgcttca ctattgatcg tcgactgatc cgcgaggatc
    30061 gccagcagaa ccacacacag cacaccggtt aagggtaaaa aactcgccag cgtagtgcct
    30121 ggtaataaag gtgtggcagg cttgcgaatg ccatctacgc ggggaatagt gcgccgccag
    30181 ctgcgccaaa acgtaggcgg cgagtaacag catcagcagc agactggcga gtgcgccttt
    30241 cgtttgttga tccgcgtcgc cctgagttaa ccactgccag ctaattactg ccagcgtcgg
    30301 tggattacct ggcccaagaa taatcgccac atccacgacc gaaagcgacc aggcaacaat
    30361 cgccagcatc gccattgcca gcgaaggcgc gacggagggc aacagcagcc agttcaggca
    30421 ttgccagcgg ctgtagccca gcgaatccag cacaatgacc tgctgcaaca gccgtttttc
    30481 gctcaatact gccactaaga tccacagcag aaatgcgctt tctttcactg caagggtgag
    30541 gcccagaccg atccaaatcg gtccattggc ggagtgaaat acgggaaata gtcatatagc
    30601 agccctccgt cagcaaagag cagcagggcg ctggtggcaa aagccacatg gggaatggcg
    30661 agcagccacg gcagacgggc gcacatacgc tgccatttcg gcccaggcca tagcgccaca
    30721 atcaccagca gggcaatcaa caatgccccg accgccgcga tggttgtcga caccagcgtt
    30781 gccagtaatg cctgcggtaa ctgtggatcg gcaaataacg cctgccagcg tgtcaacgac
    30841 aacgcaggcg atatcagcgt gagagctgcc gggatcagcg gtgcataaat caccgccacc
    30901 atcgcccaca gcaaaaagat taatgcgtac cgtaacggcg taaccattct tgttccagtg
    30961 cattcaccca tcctgcgtgc ggttcagcca gtaccggcgg cagatcctgc ggcattcttg
    31021 attgcaatat ttcgcgctgc ccgtcaggca gtttttgcgg atcgagaaca gaaggatcgc
    31081 cccagacaac gggatctgct ttacgcagtt gcgcatcggg tgagagcagg aaattggcaa
    31141 ctaccttcgc cgcagcactg gcattcgcgt tggctggagt ggtgacgaaa tgcacgttgc
    31201 caatcatccc ctcgcgaaag ccaaaactgt aactgcttgc cggcaaatcg ccgctggcga
    31261 ttttttgctg cgcatgtgcg gggttaaagg tcagcgacag gcgcaatgtg ccggctttca
    31321 gcagagcatc catccgcgcg ggcgaaggcg ggaaatcctt tccttcgcgc cacaaatacg
    31381 gatgcagtgc atcaagatat tgctacaagg gcgcagtgac acgggcgaaa gtcgcatcgt
    31441 ccggcgcttc ttttaatgcg gcgggatctg gcgtcagcat aatcagcaac tgttcaagaa
    31501 acgccgtgcc ggtaaagtcc ggtgggcgcg gataagtaac cgtgccggga ttggctttag
    31561 caaactccag taaggcttgc ggcgtttgtg gtggctgtgg cgtaacatcg cggcgggcga
    31621 taaacgtcag ttgtgcgccg ccccagggcg attccgcacc ttgtgtcggc actgaaaaat
    31681 ctccccgcac cggcagctgt gtgtcgacat agcgccagtt gggcagagtc tccgcccagc
    31741 ccgtttgtaa taaattggcc tctttta
//
LOCUS       DAJTQY010000042        31599 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.042, whole genome shotgun sequence.
ACCESSION   DAJTQY010000042 DAJTQY010000000
VERSION     DAJTQY010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31599
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.042"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(<1..606)
                     /gene="capU"
                     /locus_tag="OO349_001994"
     CDS             complement(<1..606)
                     /gene="capU"
                     /locus_tag="OO349_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HCR5867070.1"
                     /translation="MNILFTESSPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIA
                     FEASKFGIDITFALFRNSLHIPTVWRLLGIVHSFQPDAIVCHSGHDSNIVGLVRFFTW
                     KHPFRIIRQKTYLTRKTKFFSINHFCDEVIVPGTNMKTHLEQEGCRTRVTVVPPGFDF
                     QELYVDSRNSLPPSVLSWLASRRGCPVIAQVGMLRPEKGHEF"
     gene            complement(609..1451)
                     /locus_tag="OO349_001995"
     CDS             complement(609..1451)
                     /locus_tag="OO349_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCR5867071.1"
                     /translation="MLNGGGILFKANHVPVLMYHHVSHCPGLVTLSPETFRKQMKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLNEFNLKAHIFLIT
                     SFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFTSREEQCKHLRQDLLSGREYLKKMTGKCSKHLCWPEGYYNKDYIQIAEELG
                     FHYLYTTERRMNAPAKGTTRIGRISTKERENCVWLKRRLFYYTTPFFSSLLALHKGPR
                     LPDD"
     gene            1637..2354
                     /locus_tag="OO349_001996"
                     /pseudo
     CDS             1637..2354
                     /locus_tag="OO349_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            3027..4535
                     /gene="ltrA"
                     /locus_tag="OO349_001997"
     CDS             3027..4535
                     /gene="ltrA"
                     /locus_tag="OO349_001997"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCR5867072.1"
                     /translation="MQRKLATWAATDPSLRIQRLLRLITQPEWLAEAARITLSSKGAH
                     TPGVDGVNKTMLQARLAVELQILRDELLSGHYQPLPARRVYIPKSNGKLRPLGIPALR
                     DRIVQRAMLMAMEPIWESDFHTLSYGFRPERSVHHAIRTVKLQLTDCGETRGRWVIEG
                     DLSSYFDTVHHRLLMKAVRRRISDARFMTLLWKTIKAGHIDVGLFRAASEGVPQGGVI
                     SPLLSNIMLNEFDQYLHERYLSGKARKDRWYWNNSIQRGRSTAVRENWQWKPAVAYCR
                     YADDFVLIVKGTKAQAEAIREECRGVLEGSLKLRLNMDKTKITHVNDGFIFLGHRIIR
                     KRSRYGEMRVVSTIPQEKARNFAASLTALLSGNYSESKVDMAEQLNRKLKGWAMFYQF
                     VDFKAKVFSYIDRVVFWKLAHWLARKYRTGIASLMRWWCKSPEPGQSKTWVLFGKTNH
                     GKLSGEILYRLVGQGKKLFRWRLPEGNPYLRTETRNTYTSRFTEVAMAFASI"
     gene            4616..5103
                     /locus_tag="OO349_001998"
                     /pseudo
     CDS             4616..5103
                     /locus_tag="OO349_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611482.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            5186..5443
                     /locus_tag="OO349_001999"
     CDS             5186..5443
                     /locus_tag="OO349_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000177060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5867073.1"
                     /translation="MTLVNDTGFDPVFSGSIAESWRQQPCTPSYCCDWEAATMLRAFP
                     LAKKGEGRARLPSLYASFGKLGETPTHEDIIDNNRSINWPV"
     gene            complement(6001..6768)
                     /gene="fecE"
                     /locus_tag="OO349_002000"
     CDS             complement(6001..6768)
                     /gene="fecE"
                     /locus_tag="OO349_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HCR5867074.1"
                     /translation="MTLRTENLTVSYGTDKVLNDVSLSLPTGKITALIGPNGCGKSTL
                     LNCFSRLLMPQSGTVFLGDNPINMLSSRQLARRLSLLPQHHLTPEGITVQELVSYGRN
                     PWLSLWGRLSAEDNARVNVAMNQTRINHLAVRRLTELSGGQRQRAFLAMVLAQNTPVV
                     LLDEPTTYLDINHQVDLMRLMGELRTQGKTVVAVLHDLNQASRYCDQLVVMANGHVMA
                     QGTPEEVMTPGLLRTVFSVEAEIHPEPVSGRPMCLMR"
     gene            complement(6769..7725)
                     /gene="fecD"
                     /locus_tag="OO349_002001"
     CDS             complement(6769..7725)
                     /gene="fecD"
                     /locus_tag="OO349_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter permease
                     subunit FecD"
                     /protein_id="HCR5867075.1"
                     /translation="MKIALVIFITLALAGCALLSLHMGVIPVPWRALLTDWQAGREHY
                     YVLMEYRLPRLLLALFVGAALAVAGVLIQGIVRNPLASPDILGVNHAASLASVGALLL
                     MPSLPVMVLPLLAFAGGMAGLILLKMLAKTHQPMKLALTGVALSACWASLTDYLMLSR
                     PQDVNNALLWLTGSLWGRDWSFVKIAIPLMILFLPLSLSFCRDLDLLALGDARATTLG
                     VSVPHTRFWALLLAVAMTSTGVAACGPISFIGLVVPHMMRSITGGRHRRLLPVSALTG
                     ALLLVVADLLARIIHPPLELPVGVLTAIIGAPWFVWLLVRMR"
     gene            complement(7722..8720)
                     /gene="fecC"
                     /locus_tag="OO349_002002"
     CDS             complement(7722..8720)
                     /gene="fecC"
                     /locus_tag="OO349_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dicitrate ABC transporter permease FecC"
                     /protein_id="HCR5867076.1"
                     /translation="MTAIKHPMLLWGLPVAALIIIFWLSLFCYSAIPVSGADATRALL
                     PGHTPTLPEALVQNLRLPRSLVAVLIGASLALAGTLLQTLTHNPMASPSLLGINSGAA
                     LAMALTSALSPTPIAGYSLSFIAACGGGVSWLLVMTAGGGFRHTHDRNKLILAGIALS
                     AFCMGLTRITLLLAEDHAYGIFYWLAGGVSHARWQDVWQLLPVVVTAVPVVLLLANQL
                     NLLNLSDSTAHTLGVNLTRLRLVINMLVLLLVGACVSVAGPVAFIGLLVPHLARFWAG
                     FDQRNVLPVSMLLGATLMLLADVLARALAFPGDLPAGAVLALIGSPCFVWLVRRRG"
     gene            complement(8717..9619)
                     /gene="fecB"
                     /locus_tag="OO349_002003"
     CDS             complement(8717..9619)
                     /gene="fecB"
                     /locus_tag="OO349_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein FecB"
                     /protein_id="HCR5867077.1"
                     /translation="MLAFIRFLFAGLLLVISHAFAAMVQDEHGTFTLEKTPQRIVVLE
                     LSFADALAAVDVSPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDL
                     IIADSSRHAGVYIALQQIAPVLLLKSRNETYAENLQSAAIIGEMVGKKREMQARLEQH
                     KERMAQWASQLPKGTRVAFGTSREQQFNLHTQETWTGSVLASLGLNVPAAMAGSSMPS
                     IGLEQLLAVNPAWLLVAHYREESIVKRWQQDPLWQMLTAAQKQQVASVDSNTWARMRG
                     IFAAERIAADTVKIFHHQPLTVVK"
     gene            complement(9664..11988)
                     /gene="fecA"
                     /locus_tag="OO349_002004"
     CDS             complement(9664..11988)
                     /gene="fecA"
                     /locus_tag="OO349_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate transport protein FecA"
                     /protein_id="HCR5867078.1"
                     /translation="MTPLRVFRKTTPLVNAIRLSLLPLAGLSFSAFAAQVNIAPGSLD
                     KALNQYAAHSGFTLSVDASLTRGKQSNGLHGDYDVESGLQQLLDGSGLQVKPLGNNSW
                     TLEPAPAPKEDALTVVGDWLGDARENDVFEHAGARDVIRREDFAKTGATTMREVLNRI
                     PGVSAPENNGTGSHDLAMNFGIRGLNPRLASRSTVLMDGIPVPFAPYGQPQLSLAPIS
                     LGNMDAIDVVRGGGAVRYGPQSVGGVVNFVTRAIPQDFGIEAGVEGQLSPTSSQNNPK
                     ETHNLMVGGTADNGFGTALLYSGTRGSDWREHSATRIDDLMLKSKYAPDEVHTFNSLL
                     QYYDGEADMPGGLSRADYDADRWQSTRPYDRFWGRRKLASLGYQFQPDSQHKFNIQGF
                     YTQTLRSGYLEQGKRITLSPRNYWVRGIEPRYSQIFMIGPSAHEVGVGYRYVNESTHE
                     MRYYTATSSGQLPSGSSPYDRDTRSGTEAHAWYLDDKIDIGNWTITPGMRFEHIESYQ
                     NNAITGTHEEVSYNAPLPALNVLYHLTDSWNLYANTEGSFGTVQYSQIGKAVQSGNVE
                     PEKARTWELGTRYDDGALTAEMGLFLINFNNQYDSNQTNDTVTARGKTRHTGLETQAR
                     YDLGTLTPTLDNVSIYASYAYVNAEIREKGDTYGNLVPFSPKHKGTLGVDYKPGNWTF
                     NLNSDFQSSQFADNANTVKESADGSTGRIPGFMLWGARVAYDFGPQMADLNLAFGVKN
                     IFDQDYFIRSYDDNNKGIYAGQPRTLYMQGSLKF"
     gene            complement(12075..13028)
                     /gene="fecR"
                     /locus_tag="OO349_002005"
     CDS             complement(12075..13028)
                     /gene="fecR"
                     /locus_tag="OO349_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fec operon regulator FecR"
                     /protein_id="HCR5867079.1"
                     /translation="MNPLLTDSRRQALRSASHWYAVLSGERVSPQQEARWQQWYEQDQ
                     DNQWAWQQVENLRNQLGGVPGDVASRALHDTRLTRRHVMKGLLLLLGAGGGWQLWQSE
                     TGEGLRADYRTAKGTVSRQQLEDGSLLTLNTQSAADVRFDAHQRTVRLWYGEIAITTA
                     KDALQRPFRVLTRQGQLTALGTEFTVRQQDNFTQLDVQQHAVEVLLASAPAQKRIVNA
                     GESLQFSASEFGAVKPLDDESTSWTKDILSFSDKPLGEVIATLTRYRNGVLRCDPAVA
                     GLRLSGTFPLKNTDAILNVIAQTLPVKIQSITRYWINISPL"
     gene            complement(13025..13546)
                     /gene="fecI"
                     /locus_tag="OO349_002006"
     CDS             complement(13025..13546)
                     /gene="fecI"
                     /locus_tag="OO349_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FecI"
                     /protein_id="HCR5867080.1"
                     /translation="MSDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQD
                     TFLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGAPSPE
                     ERESQLETLQLLDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKA
                     VEHCLLFRLEYGL"
     gene            13844..14026
                     /locus_tag="OO349_002007"
                     /pseudo
     CDS             13844..14026
                     /locus_tag="OO349_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767718.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            14020..14124
                     /locus_tag="OO349_002008"
                     /pseudo
     CDS             14020..14124
                     /locus_tag="OO349_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            14464..14721
                     /locus_tag="OO349_002009"
     CDS             14464..14721
                     /locus_tag="OO349_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm development YmgB/AriR family protein"
                     /protein_id="HCR5867081.1"
                     /translation="MHSDYYNMVFGEKLANILYEANSQFFYERNVIEEAVNALFCERE
                     IINNKNIIKKLMFFLSDVNHTKKDVVQSALNIIIDITSGDI"
     gene            complement(15041..15172)
                     /locus_tag="OO349_002010"
                     /pseudo
     CDS             complement(15041..15172)
                     /locus_tag="OO349_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            15454..16812
                     /locus_tag="OO349_002011"
     CDS             15454..16812
                     /locus_tag="OO349_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HCR5867082.1"
                     /translation="MKRKIIPVLIGCTLSFSALAAQPTAERYVVSFPEGTHVNYAGAF
                     ASAFPNGLPVGIGSGLLFTGKQGDALTFATITDRGPNADSPKEGKNETKIFVTPDFAP
                     LLMTIRVQNGKAEAIDPRPLHDDKGAINGLPLASDVIGSTNEVAFSDTLHRLKGDNRG
                     LDTEGITPDGKGGYWLCDEYGPFLINIDSKGKILAIHGPQAAEGEKAIAGGLPNILKW
                     RQANRGFEGLTRMPDGRIIVAVQSTLDIDAKSKKKALFTRLVSFDPASGKTAMYGYPI
                     DSAAYSKNSDAKIGDIVALDNQHILLIEQGRDKNNRMRNLIYEVDLNKASDLSGFDKP
                     GEYPEFDDEKTLSQRGITLAQKTQVVDLRSLGWQQEKAEGLALIDSKTLAVANDNDFG
                     VKVAMQHPVEGKTFKDYRVNAEGKLTLDDKQVETTLRVKPLEKPESDSELWIVTLPEA
                     LK"
     gene            complement(17051..18436)
                     /locus_tag="OO349_002012"
     CDS             complement(17051..18436)
                     /locus_tag="OO349_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCR5867083.1"
                     /translation="MNDISSDDIFLLKQRLAEQEALIHALQEKLSNREREIDHLQAQL
                     DKLRRMNFGSRSEKVSRRIAQMEADLNRLQKESDTLTGRVYDPAVQRPLRQTRTRKPF
                     PESLPRDEKRLLPAAPCCPNCGGSLSYLGEDTAEQLELMRSAFRVIRTVREKHACTQC
                     DTIVQAPAPSRPIERGIAGPGLLARVLTSKYAEHTPLYCQSEIYGRQGVELSRSLLSG
                     WVDACCRLLSPLEEALHGYVMTDGKLHADDTPVQVLLPGNKKTKTGRLWAYVRDDRNA
                     GSALAPAVWFAYSPDRKSIHPQTHLACFSGVLQADAYAGFNELYRNGGITEAACWAHA
                     RRKIHDVHVRIPSALTEEALEQIGQLYAIEADIRGMPAEQRLAERQRKTKPLLKSLES
                     WLREKMKTLFFGSGHGGERGALLYSLIGTCKLNDVDPESYLRHVLGVIADWPVNRVSE
                     LLPWRIALPAE"
     gene            complement(18486..18833)
                     /gene="tnpB"
                     /locus_tag="OO349_002013"
     CDS             complement(18486..18833)
                     /gene="tnpB"
                     /locus_tag="OO349_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR5867084.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNSFNGLAAKVQTALKDDPMSGHIF
                     IFRGRSGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
     gene            complement(18830..19210)
                     /locus_tag="OO349_002014"
     CDS             complement(18830..19210)
                     /locus_tag="OO349_002014"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HCR5867085.1"
                     /translation="MQKNVTPGRRKGCPNYPPEFKQLLVAASCEPGISISKLALENGI
                     NANLLFKWRQQWREGKLLLPSSESPQLLPVTLDAAAEQPESLAEDPETLSISCEVTFR
                     HGTLRFNGNVSEKLLTLLIQELKR"
     gene            complement(19565..19999)
                     /locus_tag="OO349_002015"
     CDS             complement(19565..19999)
                     /locus_tag="OO349_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HCR5867086.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRQDVLCCVSCGCPLTLQR
                     GNDGQPPWFEHDQMTVAEKILLRCTWLDPAEKEARRLHLQGMTVPDYTVKVRKWFCVM
                     CDEDYEGEKCCPRCGTGVYSRAWGRQEVPSEDARADNPLQRL"
     gene            complement(19987..20388)
                     /locus_tag="OO349_002016"
     CDS             complement(19987..20388)
                     /locus_tag="OO349_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5867087.1"
                     /translation="MYAKSFIALDGNGRLTGARTAQAAPYANYTCHLCGSALRYHPQY
                     ETELPWFEHTDDRLTEHGQQCPYVRPERREIQLIKRLQQFVPDALPVVRKASWHCRQC
                     HHDYYGEQYCTHCQTGGFSIPRTTQEEICEF"
     gene            complement(20648..21217)
                     /locus_tag="OO349_002017"
     CDS             complement(20648..21217)
                     /locus_tag="OO349_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HCR5867088.1"
                     /translation="MTYKYNPFWQQRIRETVRHALNVHPRLTALRVDLRLPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHPTTLHYVWAREFGECKGKKHYHLMLLVN
                     RDTWCRAGDYRAPESLAGMIKQAWCSALGVDVGCHATLVHFPAWPAVWLARNDDTGFQ
                     QVLERADYLAKEHTKAHCTGERNFGCSRS"
     gene            complement(21962..22105)
                     /locus_tag="OO349_002018"
                     /pseudo
     CDS             complement(21962..22105)
                     /locus_tag="OO349_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453335.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin activation protein"
     gene            complement(22439..22705)
                     /locus_tag="OO349_002019"
     CDS             complement(22439..22705)
                     /locus_tag="OO349_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5867089.1"
                     /translation="MAEPYRYTGWLLRKDASARTRDKEGIVFVRTGREAGVLIIAELF
                     SEVVTVSQPVPGEEETDITDGIRLSDWYYKYKKSSESNVKDLPE"
     gene            22846..23052
                     /locus_tag="OO349_002020"
     CDS             22846..23052
                     /locus_tag="OO349_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCR5867090.1"
                     /translation="MATPVSLMDDQMVDMAFITQLTGLTDKWFYRLIRDGAFPAPIKL
                     GRSSRWLKSEVEAWLQARIAQPRP"
     gene            23147..23749
                     /locus_tag="OO349_002021"
     CDS             23147..23749
                     /locus_tag="OO349_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5867091.1"
                     /translation="MKQQYQTRYEWLHESYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWGSFNGEKPEIAIVPQSLHRLIYGPDKRATPPLDDDLIVNLCTSEHLLVHHPMLEG
                     ILLSECERLRQRSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNSLEDWKENLKRK
                     SEKELEEWIINRQRQSAALTDLMDQYVLLAYRTTVDDSRN"
     gene            23920..24117
                     /locus_tag="OO349_002022"
     CDS             23920..24117
                     /locus_tag="OO349_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015953093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5867092.1"
                     /translation="MFFTARYTPGSQQTECAGAVIHQKWHAAVHTHPGDNMYVATAIP
                     ALWKRLTQPAGYSFRHPGMSA"
     gene            24949..25866
                     /locus_tag="OO349_002023"
     CDS             24949..25866
                     /locus_tag="OO349_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5867093.1"
                     /translation="MVQKILSDKVMNERTNAYYSYYLGERNISVLPLNVYDPPERFIA
                     YIKKNRENLNITLSDFELEQIISGMRLKALAFLVPLEKISWIAGSERACLFSWYLLMQ
                     FIQNNRAKISADLLQKNKLYLKEEYLEGNAFPSDSSTQFRQILRVLDILSDKNLRDEW
                     IIQTKDRWMRAFKSKSPFSYLLPENEHECIWTWNYLKGKNIALEKLASFPGSADIYHA
                     IHLSFDIWVTCPLTSPDDIKNFRNSFNKAKAQRKYKKMQEDKVNVQFFLDAETRAQLK
                     ELSRVRRLSTGEMLHDLIVEEYKRYRHSR"
     gene            25951..26823
                     /locus_tag="OO349_002024"
     CDS             25951..26823
                     /locus_tag="OO349_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HCR5867094.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVQRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILHRGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQAQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDGLRTESVRGQAREQFTGAVDRIFDTAESVCVASVARKVLRAVRDT
                     VVSVARAVWNWIFF"
     gene            27041..29425
                     /locus_tag="OO349_002025"
     CDS             27041..29425
                     /locus_tag="OO349_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HCR5867095.1"
                     /translation="MHEKNIALLCDEADRLLQLNINLLRQMVDEPDVLSDSKNENRLL
                     FDKQKALKRIEELEGEQIKTARREMVLAVVGTMKAGKSTTINAIVGQEILPNRNRPMT
                     SVPTLIRHVPGKTEPVLHLEHIQPVRNLLSTLQQKLDTPAGQQVAQTLQQTGDTCELL
                     DILANDGWLKNEYHGEDEIFTGLASLNDLVRLAAAMGSEFPFDEYAEVQKLPVIDVEF
                     SHLVGMDACQGTLTLLDTPGPNEAGQPQMEVMMRDQLQKASAVLAVMDYTQMNSKADE
                     DVRKELNAIADVSAGRLFVLVNKFDEKDRNGDGADAVRQKVPAMLNSDVLPASRVYPG
                     SSRQAYLANRALHELRKNRTLPVDEAWVDDFVREAFGRMKKEYVCKDSEMATEGATDL
                     WEGSLIDQLITEVIQSSHSRAAALAVDSAAAKLMQNAENVSEYLSLRHQGLQQSIQSL
                     QSHITSLLADIREIADCQEQVTADVRMAMEEIDARTRELLTGVCTSLEEELNDYFRSG
                     KRKEQQMLEEENSAQPRERNAFAFFHDIFGTGNQHDRMRDFDPDSPEIKFSDRREALE
                     LMTQIESTVTSLHREAEAQFRPELEKIVRGIETGFRGTALYATENIAGRINARLEDEG
                     FTVKISFPAVSQLQTRLAVKTNLSALMEERTETVTRRCRQDGVWGTLCRWANTSDWGW
                     KEYSVDVSRSVININKVREEVMSLTRAYFGELQASIEQDINQPVRQEIDAFFCTFREK
                     VEQLRNTLIQSSEDHKRDQQAQERLTGRLQALNERVPELITDSKALREELETML"
     gene            29422..30327
                     /locus_tag="OO349_002026"
     CDS             29422..30327
                     /locus_tag="OO349_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HCR5867096.1"
                     /translation="MTSPFIQQIADNRVCQVLTCLPEKFVVDFANGIDVAQEHIRTAG
                     ERTFFRRLKEGLSGEGTARQNAINASLAQGVEASLRWLTELTTSLATTNYAITRVNDR
                     VSSLVSDTARLAHYSADTREQLLTLADQVHQKLNHLEEKLHRVDLVQRAQLHLEQIFS
                     WWSAGRYASFSPAGRCYVALEELRWGAFGDVIRQGETGQVNQLLDILRHKALTQMAQE
                     SGGSATVRLNTLDWLGGQGREQADNEWHDAINWLGDWCSEEQHPVIWSTTQAAEHLPV
                     RMPRLCSAERLSESMVDEIFQKGAA"
     gene            30324..31394
                     /locus_tag="OO349_002027"
     CDS             30324..31394
                     /locus_tag="OO349_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HCR5867097.1"
                     /translation="MSIEIKTGLVLSGGGAVGAYQAGVVKALAECGTQISMVSGASIG
                     AFNGAIIAASPDLSEAAVRLEALWDHLGNNQVLSVNRSVYFSLLKKLFQAMNLCQIPG
                     RAGALLTTLLRHISIINGFDNPMTQPLLSDGPLTALMDHYLDTDALAGGLPLYVSLYP
                     TEGGMQDIIDCIRAELGSGTTKNAVFQHIQSLPRGQQKEALLASAALPLLFRPREVQG
                     TMFGDGGMGGWRNMQGNTPVTPLVDAGCNMVIVSHLSDGSLWDRQAFPDTTILEIRPR
                     KRLKYAGDGGNSGGLLSFTSAHTDAWRQQGYEDTMLTMEHIRKPLAARQALSRSETVL
                     QKSLEITEEADLALRSAMARIK"
BASE COUNT         7739 a         8123 c         8349 g         7388 t
ORIGIN      
        1 aaattcgtgc cctttttccg ggcgcaacat tcctacctga gcaattacgg ggcatcctct
       61 tcgggacgcc agccaggaaa gaacactcgg aggcaaactg tttcgggaat caacgtataa
      121 ttcctggaaa tcaaagccag gaggcacaac agtaacccga gttcgacatc cttcctgctc
      181 cagatgtgtc ttcatatttg ttccgggaac aatcacctca tcgcaaaaat gatttattga
      241 aaaaaatttt gtttttcgtg tcaaatatgt cttttgcctg ataattctga atggatgttt
      301 ccaggtaaaa aaccgtacca aaccaacaat attactatca tgtccactgt gacaaacgat
      361 tgcatcgggc tgaaaactat gaactattcc gagtaatctc catacagtag gaatgtgaag
      421 actgtttcga aataacgcga atgtgatatc aattccaaac ttactggctt caaaagcaat
      481 tttgctgttc tccctgcaga caagcagaac tgaatgcccc attttcttca gggccttcat
      541 ttgagcaaca gcctgtaatt cctgaccacc tatattcggt gatgattccg taaatagtat
      601 attcattatt agtcatccgg taatcttggc cccttatgaa gggccagcaa cgatgaaaaa
      661 aacggtgttg tataataaaa aagtcgtctt ttcagccata cgcaattttc ccgttccttg
      721 gtgcttattc ttcctatccg tgtagttcct ttagcaggag cattcattct tctttctgtt
      781 gtatataaat aatggaatcc aagttcttca gcgatctgaa tataatcctt gttataataa
      841 ccttccggcc aacacaaatg tttactgcat tttcctgtca ttttttttaa atattctctc
      901 ccgctcaata agtcctgccg aagatgttta cactgttctt ctcgtgaagt gaacttttta
      961 tcccaacgtg tatgagtatg tgtatgaaca tgaaattcga ccagaccaga ttgcaacatt
     1021 tcgttcactt cagaccagcg aagcataaca ttatcagcat tcccggttgc tatttgatgt
     1081 tcgcaatcgc gatgagaata ttcctttcca ggagagtgac gaaccggacc atttccaata
     1141 aaactggtaa ttaaaaaaat atgtgccttg agattaaact cgtttaataa aggatatacc
     1201 tgaaaccagt tgtcaaggta tccatcatca aatgtcaaca taacgctttt acggggaagt
     1261 tttccgcccc gataaaaaaa ctccagttca tctgatgaca gtgttttcca gttgttttca
     1321 gccaaccatt tcatttgttt ccggaatgtc tcgggagaaa gtgtaaccag tcctgggcaa
     1381 tggctgacat gatgatacat taataccgga acatgatttg ctttaaacaa aatgccccct
     1441 ccgtttaaaa tttaaagttt ctggcaccca gtaagtagag ataaaaggaa agcctaataa
     1501 aggtggtgaa ctttatatac tactaaatca aacatcaaca atttataaac aataatatat
     1561 tatcatatct tttaaaaacg gctcactaag aaccttgagg tggcacacta aatttagcta
     1621 cctgaacaga ggtgatatgc tcacctcaga acaacacagg tgccataatg aaaaaagaaa
     1681 tttcagcgca gagtttaaac gcgaatccgc tcaactggtc gttgaccaga aatacaccgt
     1741 ggcagatgca gccagcgcta tggatgtcgg cctttccaca atgacgcgat gggtgaaaca
     1801 attacgtgat gagcggcagg gaaaaacacc aaaagcctcc cccattaccc cggaacaaat
     1861 tgaaatccgt gagctcagga aaaagctaca acgtattgaa atggaaaatg aaatattaaa
     1921 aaggctaccg cgctcttgat gtcagactcc ctgaacagtt ttcgataatc gggaaactca
     1981 gggcgcgtta tcctgtggcc actctctgcc atgtgttcgg ggtccatcgc agcagctaca
     2041 aatactggaa aaaccgtcct gaaaaaccag acggcagacg ggctgtatta cgcagccagg
     2101 tacttgaact gcatggcatc agccacggct ctgccggagc aagaagcatc gccacaatgg
     2161 caacccagag aggctaccag atggggcgct ggcttgctgg cagactcatg aaagagctgg
     2221 ggctggtcag ttgccagcag ccgactcacc ggtataagcg tggcggtcat gagcacgttg
     2281 ctatcccgaa tcatcttgag cgacagttcg ccgtaacgga accaaatcag gtgtggtgcg
     2341 gtgatgtgac ctatagtgtg cccggagttc agggcgggca tggatgctta aatgaaccgc
     2401 gagtctgtct ggaatattga accggtaact cacgatgaga aacccaacaa tcccaccggg
     2461 tgtgacggtg gagaacctga gcggcagtga cctgcggcat gcccgcaggg tgatgtaacc
     2521 cgctgacaac ggggattgag gcgagatcac taagccgaga tgatcctcaa ggttaagtac
     2581 tgaaaggctg aagaacatga acccgttaat ccgcctctgt gggttgaaaa cgtcaccacg
     2641 gcctacgtga tctgacaggc cgtgcaggag gaactggcag tgatacgtaa gcactgccgg
     2701 tcgaaggtgt tttgacatgt atgcgaaaca ccggggcagc agcgtctatc acgcttgcgt
     2761 tgctgacttc tgccaacttg cggcaagcaa ggataaagag tgcgacgggc agcctcctca
     2821 gtatgcctga gtccaggcag gtaaaccggg gaaggtcagc gacggatgtt aagggggcat
     2881 ggctccgatg acgcgctgac tggcggagct tccgtagtag tccgcgatgg ggaaagccca
     2941 ttacatggcg aagggaagca gtttgaatgt gtttgcgacg tgaattaact ggcctaacga
     3001 ggtgaagacc tttgataatc agcgaaatgc aacgcaagct tgccacatgg gcagccaccg
     3061 atccgtccct acggattcaa cggctgctgc gtctgataac acaaccagaa tggctggctg
     3121 aagcggcgcg gatcacgctt tcatcaaagg gggcgcatac ccccggcgtt gatggcgtga
     3181 acaaaacaat gctacaggcc agactggctg ttgagctgca aatcctcagg gatgaattac
     3241 tctcaggcca ctaccagccc ttgcccgcca gacgggttta catccctaaa agcaacggca
     3301 aactgcgacc actgggtatc cccgcgttgc gggatcgtat tgttcagcgg gccatgctga
     3361 tggcgatgga gccgatatgg gagagtgatt ttcatacgct ctcatatggc ttccggcctg
     3421 agcgcagtgt ccaccacgcg atccgcacgg tgaaattaca gctcacagac tgcggtgaaa
     3481 cccggggacg ctgggtgatt gaaggcgacc tgtccagtta cttcgacacc gtacatcatc
     3541 gactgctgat gaaggctgta cgccgcagga tcagtgacgc acgtttcatg actctgctgt
     3601 ggaaaaccat caaggcggga catatcgatg tcggtctctt tcgggcggcc agtgaaggtg
     3661 tgccacaggg cggtgttata tcgccgctat tatcgaacat catgctgaat gagttcgatc
     3721 aatacctgca tgagcgctac ctgagcggga aagccagaaa agatcggtgg tactggaata
     3781 acagtatcca acggggccga agtacggcgg tcagagaaaa ctggcagtgg aaacccgcgg
     3841 tggcgtactg ccgctatgcc gatgattttg tcctcatcgt caaaggcacc aaagcacagg
     3901 cggaagccat cagggaggag tgtcggggtg tgctcgaagg cagtctgaaa ctcaggctga
     3961 acatggataa gactaaaatc acccatgtta atgacggctt tatctttctg gggcacagga
     4021 tcattcgcaa acgcagtcgt tatggcgaga tgcgagtggt ctcaacgatc ccgcaggaga
     4081 aagccagaaa cttcgccgca tcgctgacag cactgttatc aggcaactac agtgaaagca
     4141 aagtcgatat ggctgaacaa ctcaaccgaa aactgaaagg ctgggccatg ttctatcagt
     4201 tcgttgattt taaggccaaa gtcttcagtt atatcgaccg tgtcgtgttc tggaagctgg
     4261 ctcactggct ggcccgcaaa taccgtacag gtatcgcttc cctgatgagg tggtggtgta
     4321 aatcaccgga accgggtcag agcaaaacgt gggttttatt tggtaaaacc aatcacggca
     4381 agctcagcgg cgaaatactg taccggttgg tggggcaagg caagaagctg ttccgctggc
     4441 ggctacccga aggtaatccc tatctgagga cggagaccag aaacacgtat acatcgcgct
     4501 ttacagaagt ggcaatggcg ttcgccagca tttaaatgga gagccggatg cgctgaaagg
     4561 tgcacgtccg gttcggggag gagaggcagg gaaatagtcc gactacgccc tgcctcttac
     4621 tctactctgg acgggtaagc gctgggcgta cctcgccgtt gttctcgacc tgttcgcaag
     4681 aaaaccagtg ggctgggcca tctcgttctc gccggacagc aggcttacca tgaaagcact
     4741 ggaaatggca tgggaaaccc gtggtaagcc cgtcggggtg atgttccaag cgatcaaggc
     4801 agtcattata cgagcaggca gttccggcag ttactgtggc gataccagat cagacagagt
     4861 atgagccggc gtggaaactg ctgggataac agcccaatgg agcgcttctt caggagtctg
     4921 aagaactaat gggtgccagc gacgggctat gtaagcttca gcgatgcagc tcacgcaata
     4981 acggactata tcgttggata ttacagcgca ctaagaccgc acgaatataa tggtgggtta
     5041 ccaccaaacg aatcagaaaa ccgatactgg aaaaactcta acgcggaggc cagttttagt
     5101 tgaccacaac agactacctg aagggagccg cggtcgccta gcagttgcag tagcaggaga
     5161 tcatccagcc gcagtacagg tcacgatgac tctggttaat gataccggct ttgaccccgt
     5221 attttccggc tctatcgctg aatcatggcg tcagcagccg tgcacaccat cctattgttg
     5281 tgactgggag gctgccacca tgcttcgcgc tttccctctg gcgaaaaagg gagaaggacg
     5341 ggcccgtctg ccttcacttt atgccagctt cggtaagctg ggtgagacac cgactcatga
     5401 agatatcatt gataacaatc gatccatcaa ctggcctgta taacgtggct gccggtgatt
     5461 aagaaagctg cacctaccta agtagtagca aacgcacact ttttagaaaa atcgatggtc
     5521 agaaactgga ttagcaattc cgttccaggg ttgcttttga tttacgttgg cgtctgatca
     5581 ttgatttatc ctcaaaagcc caacctcatt ggtaatgaac cagctccgtg aatgtccgct
     5641 ctggcacaga gcgaaatttt ttgatctccc cccctgaaat ctaaacttag tcatgtcacg
     5701 tttttgggtt tctaaaattt taacttcgcg tttttcgttg ccgtaagggt tatacagaaa
     5761 tgtccgttaa gcagagttca aaattgattg ccgtgatcac gactggtttg aaagccgcgc
     5821 ccaagcctgt acagctctgg tttgcgttga ttatgaacct gtcagcctaa agcaagcgga
     5881 tggacgatga gtattggtaa tctttcagag tccggaaaag ttcagcccca gtctgaacag
     5941 gcttgctggc gccagtccag tttcattcag tcgtggtttg gttcttacgg cctgtgcaat
     6001 ctacctcatt aggcacatcg gcctgccaga taccggctcg gggtgtattt ccgcttccac
     6061 gctgaatact gttctcagca atcctggggt catcacctct tctggtgtgc cttgcgccat
     6121 aacatgtccg tttgccatta ccaccagttg atcgcagtac cggctagcct gattaaggtc
     6181 gtgcagcaca gcgaccaccg ttttcccctg agtccggagt tcgcccatca accgcatcag
     6241 gtccacctgg tgattgatat caagataggt ggttggctca tcaagtaata caacgggcgt
     6301 attctgggcc aggaccatcg ccagaaatgc gcgctggcgc tgaccgccgg aaagctcggt
     6361 taaccgacga acggcaagat gattgatccg ggtctggttc atggcgacat taactcgtgc
     6421 attgtcttca gcggagagac gcccccagag tgacagccag ggattacgac catacgaaac
     6481 cagctcctgg actgtgatcc cctctggcgt taaatggtgc tgaggcagca gcgaaagcct
     6541 gcgggccaac tggcgtgatg agagcatatt tatgggatta tcgccgagaa atacggtgcc
     6601 agactgcggc attaaaagcc gcgaaaaaca gtttaacagc gtcgatttcc cgcaaccgtt
     6661 agggccgatc agggcggtga tcttccccgt tggcagtgag agtgaaacgt cgttaagtac
     6721 cttgtctgtc ccgtaactga ccgtcagatt ttcagttcgt aaagtcattt atcgcattct
     6781 cacaagcaac cagacaaacc acggcgcacc gataatggcg gtcagcacgc caaccgggag
     6841 ctccagtggg ggatgaataa ttctcgccag cagatcggca accaccaaca gcaacgcacc
     6901 tgtcagggct gaaacaggca gcagtctgcg gtgacgtcca ccggtgatgc tacgcatcat
     6961 atgcggcacc acgagaccaa taaagctaat cgggccgcag gcggccacgc cggtagatgt
     7021 catggcgaca gctagtaaca aagcccagaa tcgggtatgg ggcaccgaca caccgagcgt
     7081 ggtggcgcgc gcatcgccga gtgcaaggag gtcgagatcg cggcaaaaac tcaggctcag
     7141 cggcagaaat aaaatcatca gcgggatggc aatcttcaca aagctccagt cacggcccca
     7201 taagctgccg gtcagccaca gcagggcgtt gttcacatcc tgcgggcgcg agagcatcag
     7261 ataatccgtc aggctggccc agcatgcaga aagcgccacg ccggtgagcg ccagcttcat
     7321 cggctggtgg gtctttgcca gcatcttcag taatatcaac cccgccatgc cgcccgcaaa
     7381 ggccagcagc ggcagcacca tcacgggcag tgacggcata agaagtagag cccccacaga
     7441 ggccaggctg gcggcatggt taacgccgag aatatccggt gatgccagag ggttgcgcac
     7501 aatcccctgt atcagcacgc ccgccacggc gagggctgca ccgacaaaca gtgccagcag
     7561 caagcgcggc agtcggtact ccatcaatac ataataatgc tcgcgtccgg cctgccagtc
     7621 ggtcagcagc gcgcgccacg gcacggggat cactcccata tggagtgata acagcgcaca
     7681 gcccgccagg gcaagggtga tgaaaataac cagcgcaatt ttcatcctcg cctcctcaca
     7741 agccagacaa agcaagggct gccaatcagc gccagcactg cgcctgcggg cagatctccg
     7801 gggaaggcca gcgcgcgtgc gagtacatct gccagcagca tcagcgtggc ccccagcagc
     7861 atgctcactg gcagtacgtt gcgctgatcg aagcctgccc agaagcgcgc cagatgtggc
     7921 accagcagac cgataaacgc caccggacct gccacgctga cgcacgcacc aaccagaagc
     7981 agcactaaca tattgatgac caaacgtagc ctcgtcaggt tcactcccag cgtatgggcg
     8041 gtgctgtcgc tgaggttgag caggttcagt tgattcgcca gcagtaacac gacagggact
     8101 gcagtgacca ccaccggcaa gagctgccag acatcctgcc agcgggcgtg ggacactccg
     8161 cctgccagcc agtaaaagat gccgtaagca tgatcttcgg ccagcagcag ggtgatgcgg
     8221 gtaaggccca tacaaaaggc cgacagcgcg atacccgcga ggatcagttt gtttctgtca
     8281 tgggtatgac gaaatccgcc tcctgcggtc atgaccagca gccagctcac gccgcccccg
     8341 catgccgcga tgaacgacag agaatagcct gcaatcggcg tcggactcag cgcgctggta
     8401 agcgccatag ccagcgccgc gccgctgtta atgccgagca gtgaaggaga ggccattggg
     8461 ttgtgggtca gggtttgcag cagcgtgccc gcgagcgcca ggcttgcgcc gatcagaacg
     8521 gcgaccaggc ttcgtggcaa acgaaggttt tgcaccagcg cttctggtag cgttggcgtg
     8581 tgtccaggca gcagggcgcg ggttgcatct gctccggaaa caggaatggc cgagtagcaa
     8641 aacagactca gccagaaaat aataataagt gctgcaacgg gaagccccca cagcagcatc
     8701 gggtgtttta tcgcggtcat ttcacaacgg taagcggctg atggtggaag atttttaccg
     8761 tgtcagcggc aatacgctct gcagcaaaaa taccgcgcat ccgcgcccag gtgttactgt
     8821 cgaccgaagc aacctgctgc ttctgcgcgg cggttaacat ctgccagagc ggatcttgtt
     8881 gccagcgttt aacaatgctc tcttcgcgat agtgggcaac cagcagccag gcaggattga
     8941 ccgccagcag ttgctccagg ccgatggacg gcatggacga acccgccatc gcagcgggaa
     9001 cgttcagccc cagagaggcc agcacgctgc cggtccaggt ctcctgagta tgcaggttga
     9061 attgctgttc gcgtgatgtg ccaaaggcca cgcgtgtccc tttgggaagc tggctggccc
     9121 actgcgccat cctctcttta tgttgttcca gacgtgcctg catctctcgc tttttaccca
     9181 ccatttcgcc gatgatagcc gcagattgca aattttcagc gtaggtttcg ttgcgggact
     9241 taagcagcag taccggcgcg atttgctgca aggcgatgta aacccccgca tggcgactgc
     9301 tgtcggcaat gatcaggtct ggtttcagag cggcaatggc ttccaggctc ggctgcgcgc
     9361 gcgttccgac ggactgccac ggtttcaggt gcgcacgcac ttcgggcagg atgcgttttg
     9421 catcgttatc gtcggcaata ccgatcgggc tgacgtccac ggcggccagc gcatcggcga
     9481 acgagagttc cagcaccaca atccgttgtg gcgttttttc gagtgtaaac gtgccgtgtt
     9541 cgtcctgaac catggcggca aaggcgtggc tgatcaccag cagcaggcct gcaaaaagaa
     9601 aacggataaa tgccaacata atcacattcc agctaaaagc ccggcaagcc gggcgttaac
     9661 acatcagaac ttcaacgacc cctgcatata cagcgtgcgc ggctggcctg catagatgcc
     9721 tttgttgttg tcgtcataag agcggatgaa gtagtcctgg tcgaagatgt ttttcacacc
     9781 gaatgccagg ttcagatctg ccatctgcgg gccaaagtca tacgccacgc gtgcacccca
     9841 gagcatgaag ccgggaatgc ggccggtact gccgtcggcg ctctctttca ccgtattggc
     9901 gttatccgca aactggctgg actggaaatc gctgttcaga ttgaacgtcc agtttcctgg
     9961 cttgtagtcc acgcccagcg tgcctttatg tttcggggag aatggtacca gattgccgta
    10021 ggtgtcgcct ttctcgcgga tttccgcgtt cacatacgca tagctggcgt agatggaaac
    10081 gttatcaagc gttggcgtta gcgtacccag atcgtaacgt gcctgcgttt ccagcccggt
    10141 atggcgcgtt ttgccacgtg cagtgacggt gtcgttggtc tggttggagt cgtactgatt
    10201 gttaaagtta atcaggaaca gccccatttc cgccgtcagc gcgccgtcgt cgtagcgggt
    10261 accgagttcc caggttcgcg ctttttccgg ttccacattg ccgctttgca cagccttgcc
    10321 aatctggctg tactgtacgg tgccgaacga gccttcagtg tttgcataaa gattccagct
    10381 gtcagtcagg tgatagagca cgttcaacgc cggaagcggt gcgttatagc tcacttcttc
    10441 gtgcgtgcct gtgatggcgt tgttctggta tgactcgata tgttcgaaac gcatacccgg
    10501 cgtgatggtc cagttgccga tgtcgatttt gtcatccaga taccaggcgt gcgcctcggt
    10561 gccggaacgc gtatcgcggt cgtaagggct tgagccggac ggcaactgcc cgctgctggt
    10621 ggcggtgtag taacgcattt catgcgttga ttcattcaca tagcgatagc ccacgcccac
    10681 ttcgtgcgcg gaagggccga tcataaagat ctggctgtag cgtggctcaa taccgcgcac
    10741 ccagtagtta cgcggcgaga gggtgatgcg tttgccttgc tccaggtagc cgctgcgcag
    10801 ggtttgggtg tagaacccct gaatgttgaa tttatgctgg ctgtctggct ggaactggta
    10861 gcccaggctc gccagcttgc gacgacccca gaagcggtca tacgggcggg tggattgcca
    10921 gcgatcggcg tcgtaatccg cgcgagacag gccaccgggc atgtcggctt caccgtcgta
    10981 atattgcagc aggctgttga aggtgtgcac ctcatccggc gcatatttgc ttttcagcat
    11041 caggtcgtcg atgcgggtgg cgctgtgctc gcgccagtca ctgccgcgcg tgccggagta
    11101 gagcagcgcg gtgccaaaac cgttgtccgc tgtgccgccc accatcaggt tgtgcgtctc
    11161 tttcgggttg ttttgtgaag aggttgggct gagctgacct tccacgcccg cctcgatacc
    11221 aaagtcctgc ggaatggcac gggtaacaaa gttcaccacg ccgcccacgc tctgcggtcc
    11281 gtaacgcacc gcaccaccac cgcgtaccac gtcaatggca tccatgttgc cgagcgaaat
    11341 gggagccagt gaaagctgcg gctgaccgta aggggcgaag gggacgggga tgccgtccat
    11401 caggacggtc gagcggctgg cgaggcgcgg gttcaggccc cggatgccaa agttcatcgc
    11461 caggtcgtgg ctgccggtgc cgttgttttc cggcgcgctg acgccaggga tgcggttaag
    11521 tacctcacgc atggtggttg cgccggtttt ggcgaaatcc tcacggcgga tcacgtcacg
    11581 cgcgccagca tgttcaaata cgtcgttttc acgcgcatca cccagccagt cgccgaccac
    11641 ggtcagggca tcttcttttg gtgcgggcgc gggctccagc gtccagctgt tatttcccag
    11701 cggttttacc tgcagtccgc tgccgtccag cagttgttgc aggccgctct cgacgtcgta
    11761 atcgccgtgc aggccgttgc tctgcttgcc gcgcgtcagg ctggcgtcaa ccgagagggt
    11821 aaatccgctg tgtgcggcat actgattgag cgctttatcg agcgatcccg gtgcgatatt
    11881 aacctgtgca gcaaaagcgg aaaacgagag accggccagc ggcagcaggc tcaggcgaat
    11941 ggcgttaacc aaaggtgttg ttttacgaaa aacgcgtaac ggcgtcatac cttccccatc
    12001 atcatttttg ttgtgttcag ctatgagtcg aacgagaagg gtaaaaagga caatcgaaat
    12061 aagaattatt ttccttacag tggtgaaatg tttatccagt accgcgtaat agactgaatt
    12121 ttaacgggaa gcgtttgcgc gataacgttc aggatcgcat cggtattttt cagcgggaac
    12181 gtcccgctca ggcgcagccc ggcaacggcg ggatcgcagc gcagcacgcc gttgcggtaa
    12241 cgggttagcg tggctatcac ctcacccagc ggtttatcgc tgaagctcag gatgtccttc
    12301 gtccagcttg tactctcgtc atccagcggt ttcactgcgc caaactcaga ggcgctgaac
    12361 tgcaggcttt caccagcgtt cacgatgcgt ttttgcgcgg gggcactggc gagaagcact
    12421 tccacagcgt gctgctgaac gtcaagctgc gtgaaattat cctgctggcg gacggtaaat
    12481 tctgtcccta aagcagtgag ctggccctga cgggtcagga cacggaaggg gcgttgcagg
    12541 gcatctttcg cggtagtaat ggcgatttca ccgtaccaga gccggacggt gcgctgatgc
    12601 gcatcaaaac gcacatccgc cgcgctttgg gtattcagcg tgagcaggga gccatcttcc
    12661 agttgctgac ggctgactgt gcctttggcg gtgcggtaat ctgcccgcag accttcgccg
    12721 gtttccgact gccagagctg ccagcctccg ccagcgccga gcaacagcag taatcctttc
    12781 atcacgtgac ggcgggtgag gcgggtatcg tgcaacgccc ggctggcaac gtcgccaggc
    12841 acaccgccaa gctggttgcg caggttttca acctgctgcc aggcccactg gttatcctga
    12901 tcctgttcat accactgttg ccagcgcgct tcctgttgtg ggctgacgcg ctcgccgctt
    12961 agcacggcat accagtggga agctgaacgc agcgcctgac ggcgggaatc ggttaacaaa
    13021 ggattcataa cccatactcc agacggaaca gcaggcagtg ctcgacggct ttcgccacgt
    13081 attttttcac ggagctgatg gaaacaccga gtttgtgcgc aatctcgctg tatgtcagac
    13141 catccagttg cgaaagcaga aacgcttcac gtgttttgcc gtttagcccg tccagcatgc
    13201 tgtcgaggag ttgtagggtc tcgagttggc tttcgcgttc ctcaggtgaa ggcgctcccc
    13261 cctccggcat aagcgccagc atctccagat acgctttttc cagcgcgttt cggcgaaaca
    13321 ggtccaccat cacgcgtttg gcgatagtgc agaggaagga gcgaggatcg cggatcgtcg
    13381 agagcgtttc gctgaccatt acccgcaaaa aagtgtcctg ggcaatgtca tctgcatcaa
    13441 aagcagactg gagtttgcgc gtcagccagc ttttcaacca gccgtgatgt gtgccataaa
    13501 gcgactcgaa cgttaaggaa gctgtggtag tggcgcggtc agacatgcgg agtgcatcaa
    13561 aagttaatta tcacgtagtc atattaatat gagaatggtt atcattacaa ttggaaataa
    13621 aattgtttcc aatagacatt tttaacatgt tgtttttcta agtgttataa ggtaggtata
    13681 aaatgggatg gagcctctgc ttctgacatg tgtcggtcag aatgactcat gatgtggtct
    13741 gctattattg acatcctcac tgccctaaag gatggggatt tcggtaatga tcttacccag
    13801 caatagtgga cacgcggcta agtgagtaaa ctctcagtca gaggtgactc acaagacaaa
    13861 acagtttcaa ccagtaaaaa aacgtaaaca ccattcgcat gaatttcgta gttaagatct
    13921 gaagcttgca gaatgcatcg gtgttactgc cggagtccgt gaacttagtc tgcatgaatc
    13981 accacagctt tacatctgtc gcagtaaaca gcaaaatcag caaacttact taattatatc
    14041 gagtgcgatt acaatcgctg gcgatggtac agttggtgtg gcggtctcag tccggaacaa
    14101 tttgaaaatc ataatctcac ttaaaacggc atccatttta caaaagatgg ccgtctgtgt
    14161 atttgtaatg aataccttct acatgtttag agtgcataat ccacgacaag gcgatatcct
    14221 aaaaattagc tagcaggcaa ctttgatggc atagataatg tttttagtaa aaaagagttg
    14281 actgaatata atacagcctc ttcctacata agaaggaatg tgctcacaaa aaaaaaccat
    14341 tagtctgctt cctgttctta caataatcag tattatgtat tttatttata tagcaaaata
    14401 tgcttgttac acctgtcata attaggcatg gtaaaattat tgactgaacc ctgggggaga
    14461 aagatgcaca gtgattatta taatatggtt tttggtgaaa aattggccaa tattttatat
    14521 gaagctaact ctcaattttt ttacgagaga aatgtcatag aagaggcagt caacgcttta
    14581 ttctgtgagc gtgaaataat aaacaacaag aacataataa agaagctgat gttctttttg
    14641 tcagatgtta atcacaccaa gaaagatgtt gttcagagcg cacttaatat tatcattgac
    14701 attacatcag gtgatatttg agattttaca taaaagataa tctctcaagg agtcttatct
    14761 gaaaacgaaa tatatagtcc ctctcgtttt gtaatatcga ttggctttgt tgaatcggtt
    14821 acgtaccccg gagccggtca tattttcagg taatacacac gctttcagac atttcggctt
    14881 tcgtcatttt tgtatcatgg gcatagcttc tgctacctga ccatcataat cacgcagctt
    14941 cagtgaagca gtgaccacct gtttaacccg gtgtatcgtt atgatcttat ccagcaattg
    15001 aaccgccccg ggtttcctgg agagtgtttt atctgtgaac tcaggctgcc agatcatcgt
    15061 ttccgatgga agcataataa gctttttctg cttctgccgg tgggatatgg ccctgccttt
    15121 ccagcaatcg tcgattgtta taccagtcca cccacgtgag tgtggtcagc tcatcatact
    15181 gacacgtgca ttacccctac cgcgataatg caaccgtaca atatagggcg tttcctaaac
    15241 gcagccctca tcactccaat ccaacaagac acagcatttt tgatcacgaa gaaagacaaa
    15301 taatttccgt aaatgtttcc gttgttacaa ataacaacag tgttttacat ttctttggcc
    15361 tcaggcccgc gcatgttgcc attcgatgtc attttcctga ctcgtttctt ctttatggtg
    15421 tagtgtgatc ttcactttat agatgctaac aatatgaaaa gaaaaattat tcccgtgcta
    15481 atcggttgta cgctctcttt ctctgccctg gcggcacaac ctacggctga gcgctatgtc
    15541 gtcagttttc cagaaggcac gcacgtgaac tacgccggcg catttgccag tgcattcccg
    15601 aacggccttc cggttgggat aggttctggc ctgctgttca ccggtaaaca gggcgatgcc
    15661 ctgacattcg caaccattac cgatcgcggt ccgaacgcgg attcaccgaa agaggggaaa
    15721 aacgaaacta aaattttcgt tacgccagat ttcgcgccgc tgctgatgac gattcgtgta
    15781 caaaacggca aagcggaggc catcgatccc cgtcctctgc atgacgataa aggcgcgatc
    15841 aacggtctgc cgctggcaag cgacgtgatt ggctccacca atgaagtggc cttcagcgac
    15901 acgttacata gactgaaggg ggataatcgc gggctggata cggaagggat cactccggat
    15961 ggtaaaggag gttactggct gtgcgatgag tatggcccgt tcctgattaa catcgacagt
    16021 aaagggaaaa tcctggcaat ccacggtccg caggcggcgg aaggggaaaa agcgattgcg
    16081 ggcggtttgc caaatatcct caaatggcgt caggcgaacc gcggctttga aggcctcacc
    16141 cgtatgccgg acgggcgtat tattgtcgcc gtgcaaagta cgctggatat cgacgctaag
    16201 agcaagaaaa aggcgctgtt tacgcgtctg gtgagcttcg atccggcaag cggaaaaacc
    16261 gcgatgtacg gttatcctat cgacagcgcg gcctacagca aaaacagcga cgctaaaatc
    16321 ggagacatcg tggcgctcga taaccagcac atcctgctga tcgaacaggg aagagataaa
    16381 aacaacagaa tgcgtaacct gatttacgag gtcgatctca ataaggcgag cgacctgtca
    16441 ggcttcgaca agccgggtga gtatccggag tttgatgatg agaaaaccct gtcacagcgc
    16501 ggcattaccc ttgcgcagaa aacgcaggtt gttgatctgc gctccttggg ctggcagcag
    16561 gagaaggctg aagggttggc gctgatcgac agcaaaacgc tggcggtggc gaacgataac
    16621 gactttggcg tgaaggtggc gatgcaacat ccggtcgagg gcaagacatt caaggattat
    16681 cgggtgaacg cggaaggaaa gctgacgctt gacgacaagc aggttgaaac gaccctcaga
    16741 gtgaagccac tggagaaacc ggagtctgac agtgaactgt ggattgtgac gctgccggaa
    16801 gcgttgaaat aagtttataa ggtacggaca gcagtatcgc ttagtttttc aaattcatgt
    16861 atgtgaacaa tattggggtt ccggcgtaga cccccaatga aagcgtcacc acgtgaaaac
    16921 atgcctgcac ggtttacacg tcatcccatg gtggcagccg gttaactttc ctgcgccacc
    16981 agtggaaaaa ggccataagc agtccgaaca aaagtaagcg tacagcgagg gccgtattga
    17041 cggggatgtg ttattcagct ggcagtgcta tgcgccacgg aagcagttcg ctgacccggt
    17101 tgaccggcca gtctgctatg acgccaagca catggcgaag gtagctttct ggatccacgt
    17161 cattcagttt gcacgtcccg atcaggctgt acagtagcgc tccccgctca ccaccatggc
    17221 cagagccgaa gaacagggtc ttcatctttt cacgcaacca gctttccagg gatttcaaca
    17281 gcggtttcgt ttttcgctga cgttcagcaa gccgctgctc tgccggcatt ccccttatat
    17341 ccgcctctat ggcgtacaac tgaccgatct gctccagggc ttcttccgtc agtgctgacg
    17401 ggatgcggac gtgcacatcg tggatctttc ggcgggcatg agcccagcag gcagcttccg
    17461 ttatcccacc attgcgatac agctcgttga acccggcgta cgcatccgct tgcagcacac
    17521 cgctgaagca agcaagatga gtctgcggat ggatgctttt tctgtccggg ctgtaagcga
    17581 accacactgc aggtgccaac gctgacccgg cattgcggtc atcacgaaca tacgcccaca
    17641 accgcccggt cttcgtcttc ttattacccg gcagcagtac ctggaccggg gtatcatcgg
    17701 catggagttt gccgtcagtc atgacatagc catgaagcgc ctcttccagc ggagacagca
    17761 gccggcagca tgcatccacc cagcccgaca gcagtgaacg gctcagctcc acaccttgcc
    17821 ggccgtatat ttctgactgg caatacagcg gggtgtgctc tgcatacttc gaggtcagca
    17881 cgcgggccag cagccccggt ccggcgatac cccgctcgat gggccgcgaa ggtgcaggtg
    17941 cctgcacgat ggtatcgcac tgagtacagg catgtttttc ccgtaccgtc cggataaccc
    18001 ggaaggcgct acgcatcaac tccagctgtt cggcggtatc ctcgcccaga tagctcagtg
    18061 aaccgccgca gttcgggcag cacggcgccg caggcaacag tcgcttttcg tcacggggta
    18121 gtgattcagg gaacggctta cgggtgcggg tctgacgcaa cggacgctgt actgccgggt
    18181 catacaccct accagtcagc gtatcgctct ctttctgaag ccggttcaga tcggcttcca
    18241 tttgtgcgat acggcgggag actttttcgg aacgactgcc gaagttcatc cggcggagtt
    18301 tatccagctg cgcctgcaga tggtctattt cgcgctcccg gttgctcagc ttttcctgca
    18361 gggcgtggat cagcgcttcc tgttcggcca ggcgctgttt cagcaggaag atgtcgtcag
    18421 aagagatgtc gttcataagc ccgtatttta ccgggcttat tctgtgacaa ccaggataaa
    18481 gagatttaca gcatggtcag ggaggtcagc agccgcttgg gctgtcgcca gtcgatacct
    18541 tccagcagca tcgccagctg cgcctgcgta aggaacactt tgccatcacg ggctgacggc
    18601 caggcgaagc gcccacgctc cagccgtttg gtcaggaggc acagtccgtc gccggtggac
    18661 cacagcagtt taacctgact gccgctgcgg ccccggaaaa tgaaaatatg gccggacatg
    18721 ggatcgtctt tcagcgccgt ttgtactttc gcagccaggc cgttgaagct atttctcata
    18781 tcggtgatac cggcaaccag ccaaattttg gtcccggaag gtaacgggat catcgcttca
    18841 gttcctgtat cagcagagtc aggagctttt cgctgacatt gccattgaag cggagcgtcc
    18901 cgtgccggaa cgttacctca cagctgatac tgagggtttc cgggtcctct gcgagcgatt
    18961 ctggctgttc ggcagctgca tcgagagtca caggaagtag ctgggggctc tctgaagaag
    19021 gtaatagcag ctttccctcg cgccattgtt gtcgccattt gaacaacaga ttggcgttaa
    19081 tgccattttc aagagcaagt tttgagatgg atatcccggg ttcacaggag gcagcaacga
    19141 gcagctgttt aaattcggga ggataattag ggcagccttt tcgcctgccg ggagtcacat
    19201 ttttctgcat atctgacact ttggttccca ctacttattt ggtggacacc actttgtcta
    19261 attcgtcaga ttctgaccag acggttcagg ctgtacgctt accactattt tatccctgtt
    19321 gaatttggtt aagccggtgc agacgcgccg ggaaacgcac acctgaaccg gcacaataaa
    19381 atacaataca accccctgtt actggagata cgaatggaat gacgaggcag cctgtcagtc
    19441 acagggggct gttggcataa aatcaacgga aaggccacca acaccggaat taagctccac
    19501 agtccccgca ctggcaatcc attcccggga aaggcatcac cgcaaactgc tccggaggca
    19561 accattacag cctctgtaac ggattatcag ccctggcatc ttccgacggc acctcctgcc
    19621 gcccccacgc cctgctgtat accccggtac cgcagcgtgg gcagcacttt tccccctcat
    19681 aatcttcgtc acacatcaca caaaaccact ttctcacctt caccgtataa tccggaaccg
    19741 tcatgccctg cagatgcaaa cgacgggcct ctttctctgc cgggtcaagc caggtacatc
    19801 gcagcaggat tttttcagcg acagtcatct ggtcatgttc aaaccacggt ggttgtccgt
    19861 cattgccccg ctgaagtgtt aacgggcatc cacaggacac acagcacaaa acgtcctgtc
    19921 ggttgtgctt cgcagcttct ttcgccgtaa caaaatggcc tttgctgtca ttcgccatat
    19981 aacagttcag aattcgcata tttcctcctg agttgtccgg ggaatactga atcctccggt
    20041 ctggcagtgt gtgcagtact gctccccata ataatcgtgg tgacattgtc tgcagtgcca
    20101 gctggcttta cgcaccacgg gtaaggcatc cggtacgaat tgctgcagac gtttaatcaa
    20161 ctgtatttct ctgcgctccg gcctgacata agggcactgt tgaccgtgct ctgtcagcct
    20221 gtcgtcagtg tgttcaaacc agggaagttc agtttcgtat tgcggatggt atctgagtgc
    20281 actgccacac aagtggcagg tgtagttagc ataaggtgcg gcctgtgcag tacgggcgcc
    20341 cgtcagacgt ccgttgccat caagagcgat aaaggatttt gcgtacatag tcatatacct
    20401 cagccgccag acgacacgca ggcaacagcc gtcccggtac gggcagcgtg gtcagggtgt
    20461 gaatggcggt tcagttaaaa tctggactgg agtttgggta aagcaggctg gttgctgtta
    20521 cagcactgtg gttgtcacac aatggaacac tgacgtaaaa aacagtcagt gggcgagtgg
    20581 atacatcgtt gccgccagac aacaacccat acttttggtt tgccggatat tggcgcgcca
    20641 gtcgtgctca actccggcta cagccaaaat tgcgctcacc ggtgcagtga gctttggtat
    20701 gctccttcgc cagatagtca gcacgttcca gcacttgctg aaagccggtg tcatcattac
    20761 gcgccagcca caccgccggc caggccggaa aatgcaccag cgtggcatgg cacccgacat
    20821 ccactcccag ggcactgcac cacgcctgtt taatcatccc ggccagagac tccggagcgc
    20881 ggtaatcacc ggcacgacac caggtatccc ggttgaccag cagcatcagg tgatagtgtt
    20941 ttttaccttt gcactcccca aactcccggg cccagacgta atgcagggtt gtgggatgca
    21001 cgcgtttacc ttcccgatgc ttacgtttct ggtaagcgtc gattcgggct ttcagggcat
    21061 tgatgaagcg ggatatcaca gctgcgtccg ttgctgccgg tacatccggg agacgcaggt
    21121 caacccgcaa tgccgtcagg cggggatgaa cattcagtgc gtgccgcacc gtctcacgaa
    21181 tacgttgctg ccagaagggg ttgtatttgt aggtcatggt taaatctccg tatggttcat
    21241 acggaatagc cacgtcgtaa aaaatacgca gagtccctga agtgacctcc ggcaggacac
    21301 tgtctcaggc actctgtgat aacccgaacg gtcgtctccg gactgacggt tgaattttca
    21361 gcgtcgtttt ttaatgtaaa aaaccggtta cgggtaaggc tgtgaggagg gagtgatggt
    21421 gatacgcaaa aagaagtgca gggactgtgg aaacgcgatt actcataata cagtgtgttg
    21481 cccgtattgt ggttctgtcg accccttcgg ctactaccgg aatacagacc gtatcgttac
    21541 catactgctg gcgctgatta ttgtggtcct gctgactact gtcagcgtca gtgtgtgtat
    21601 actgtgtagc tggtgagata acggttcatg tatcccctga gctgacagtg taccgtgtat
    21661 attaagcgta accgacaggt tctccttcat accatattaa attttccagg gattgccgct
    21721 gccttatttc gtcccataca ggaaaactca tctgggtttc tgacaaatac gcgaaactca
    21781 cagcccctca atatcaacca cacgttgtaa gccggtaata cctgaaaatc agcctattac
    21841 cgcccacgcc tccggcacca ttcccttttg atttcgaatt ctttgttgtc gctataccgc
    21901 ttaatgttcc cggtcctccg gtgcagataa caccggagtc atcgtagcgc tgcttcaata
    21961 ttcaggtgac gtatttccag acggaagccg gcgcgcgatc gtaaagtttg ttcattgtca
    22021 gttctgccag ccggttgtca acggcagagt taaaggcttc cagctccgca agggtaagtt
    22081 tataacgcgt gtgggaattc acttttcccg cgtctcctga cgtattacgg gctgccactt
    22141 acctggtgtc ccgcaataca gccttagtgt tccgttatcg gggatccgga cgcaccgcaa
    22201 caaccgggga agtatttctg gtttagagca gtgtcaagag ggatttctca gcccctcact
    22261 caccggctca gtctcagggc aggagcatta atacgctcag ttcatgtctt atacaggaat
    22321 gggcacaggg ggagctggcg tgtcactgct gattttacgc atcagcgttc atgtatctgc
    22381 actctgatac atgaacgatt cacagcgctc tgttaccgga gagatttact ctcttagctt
    22441 actctggcaa atcctttacg ttactctctg atgacttctt atatttataa taccaatcag
    22501 acagccttat cccatcggtg atatcagtct cctcctcgcc aggaacgggc tgactcactg
    22561 taactacctc cgaaaacaac tctgcaatta tcagaacgcc tgcttctctc cctgtcctca
    22621 cgaaaactat cccctcttta tcgcgcgtgc gtgcggaagc atcttttcgc aacaaccacc
    22681 cggtataccg gtacggctca gccaccgcaa cctccccgtt tatctcctgg cggtcacatt
    22741 cccgattatt ttttacgttt ctccccggtt gttatgccgg tgaaggtggc gcgctgtttt
    22801 catgaccaca ccggctggtt taacaacatc cggaggaaca ttctcatggc taccccagtt
    22861 tcactgatgg atgaccagat ggtcgacatg gcatttatca ctcaactgac cggcctgaca
    22921 gataagtggt tttacaggct catcagggat ggggcctttc cggctcccat caagctgggc
    22981 cgcagctccc gctggctgaa aagtgaagtg gaagcctggc tacaggcgcg tattgcacag
    23041 ccccgcccgt aatttctgac cgttatccgt tcacccgcag cagacgtaat ccccggcctg
    23101 ccggcggcat tctgctgcct gttatatccc cgtgaggaat atgaaaatga aacaacagta
    23161 tcagacccgc tacgaatggc tccacgaaag ctaccagaaa tggctgaccg gcttcacccg
    23221 gcacgccgta tcctggggcg tgtgtcatcc gaatatctac tacttccata atctgacgcc
    23281 cggttggggg tcattcaatg gtgaaaaacc ggagattgcc atcgtacccc agagcctgca
    23341 ccggctgatt tatggccctg acaaacgggc cacgccgccc ctggatgatg atctgatagt
    23401 gaatttatgc accagtgaac atctgctggt tcatcatccg atgctggaag gcattctcct
    23461 gtctgaatgt gaacgtctga ggcagcgttc tctggcaaat aaactgatca gcctcttccg
    23521 tcagtttggt ggcacggagt tgcgcctcaa actggtctgg ctttgctggc ttgatttaat
    23581 gaccggaaac agccttgaag actggaaaga gaacctgaaa cggaaatcag aaaaagagct
    23641 ggaggaatgg atcatcaacc gtcagaggca gagcgcagca ctgacggatc tgatggatca
    23701 gtacgtgctc ctggcatacc gcacaacggt tgacgatagc cgcaactgat gccggttgct
    23761 tccggaaggt gtgacaaaat acggaaatga tgatgctccc ggcccccatg ctcacctgca
    23821 gagcgtgttc gtcgtcatca tactgagcca tgacgggcgt cttaagggca tcctgtcatt
    23881 gctgagcagg caacaccacc agaggagcca cggacaggga tgttctttac agcccgatac
    23941 acgccaggtt ctcagcagac tgaatgtgca ggtgcggtta ttcaccagaa atggcatgct
    24001 gcggtacata cccatcctgg tgacaatatg tacgtggcga cagcgatccc ggcactgtgg
    24061 aagagactca ctcagcctgc aggatattct ttccggcatc cgggaatgtc cgcctgaatg
    24121 caaacaggat gatatttgaa tccgtaatac tgttctttca tcataaataa tttatgcaga
    24181 tacagttaac cgttacagta gccggtttgt tttaccggga aataatatcc gtcagactga
    24241 taccggaaca tatctgaaac acgttatgat gttttgttat cagaaatgtt gtatattctt
    24301 atccgtggcg acagtttaat gccctctggt atcacctgat aatgtatatc ctgcgatgat
    24361 tcgttatatt tacgggacac gcctcttttc attgtgctcc ttttcagact tcattttccg
    24421 taagtgttcc ccggcataaa ggaggtacct ttctggcgtt tataactctc tttatcggtg
    24481 acgccggtaa tacagggaat accacaccga caaacgaggt attaacagac gatatttcag
    24541 tgtccataat ttttctgtcc ggttaaataa cgtcagtctg ctgccctctg actgtaatca
    24601 tcaaagccgg aatgcatacc gggttacaac gtaaaaaaag tgatgctggc ccccggtgca
    24661 gctggcgggc gaaacatgtt tacctttcaa taccccctgt ctgcagtctc cactcttcca
    24721 tcctcttaag ctacgtcggc cacatgagtg cacttccatg ataacaggta ttcctgtcag
    24781 cataacgaag ccggttacct tcccttcctc ctgcatttcc tgtcagaatg actatacagc
    24841 gcgcagattg cctttttatt gaccatctta atataccgtt ataaacttct ttttatcata
    24901 ttattatcac cttattatgt ttaattttta agcagtcgga gaaagaggat ggttcagaaa
    24961 attttgtcgg ataaggttat gaatgaaaga acaaatgcgt attactctta ttatcttgga
    25021 gagcgtaata tctccgtact gccccttaat gtttatgatc ccccggaacg ttttatcgct
    25081 tacataaaaa aaaacagaga aaatctgaac atcacacttt cagattttga acttgaacaa
    25141 ataatttctg gtatgcgtct gaaggccctg gccttcctgg tccccctgga aaaaatatcc
    25201 tggatcgcgg gcagtgaacg cgcatgctta ttttcctggt atctgttgat gcagtttatc
    25261 cagaataaca gggcaaaaat aagcgcagac ttgcttcaaa agaataaact gtatcttaaa
    25321 gaagagtatc tggaagggaa tgccttcccg tcggactcat cgacacagtt cagacaaata
    25381 ctcagagttc tcgatattct ttctgataag aatttgcggg atgagtggat aatccagacc
    25441 aaagacagat ggatgcgggc atttaaatca aagagtcctt tcagttacct cctcccggag
    25501 aacgaacatg aatgcatatg gacatggaat tatttgaagg gaaaaaatat tgcactggaa
    25561 aaactggcca gttttcctgg ttctgctgat atttaccacg caattcacct ttcatttgac
    25621 atatgggtta cctgtccact cacctcacct gacgatataa aaaatttcag aaacagtttc
    25681 aataaggcca aggcgcagcg caagtacaaa aaaatgcagg aagataaagt caatgtacag
    25741 ttttttcttg acgcagaaac cagggcgcag ctcaaagaat tatcgagggt cagaagactc
    25801 agtaccggcg aaatgctgca tgacctgatt gttgaagagt ataaaaggta ccgacattcc
    25861 agataatttc agacatatat tatctccgtg agtcccccgc cacctttccg gtgtgcgggg
    25921 gttttgtctt ttttcaccgg gaatacatgt atgaatccgt ctgatgccat tgaggcaatt
    25981 gaaaaaccgc tctcctccct gccttactca ctttcccgtc acatcctgga acatctgcgc
    26041 aaactcacca gtcacgaacc cgtgattggc attatgggta aaagcggggc cggtaaatcc
    26101 tcactctgta atgcactgtt tcagggggag gtcaccccgg tcagtgatgt tcacgccggc
    26161 acccgggaag tgcagcgttt ccgtctgagt ggccatggtc acagcatggt tatcactgac
    26221 ctgcccgggg tgggcgagag ccgggacaga gatgcagagt atgaagccct gtaccgtgac
    26281 attctgcctg aacttgacct ggtactgtgg ctgattaaag ccgatgaccg tgccctgtct
    26341 gtggatgagt atttctggcg acacatcctg caccggggac atcagcaggt gctgtttgtg
    26401 gtgacgcagg ccgacaaaac ggagccctgc catgaatggg atatggccgg tattcagcct
    26461 tctcccgcac aggcacagaa tattcgcgaa aaaacggagg cggtattccg tctgttccgg
    26521 cccgtacatc cggttgttgc cgtatcggcc cgcaccggct gggaactgga tacgctggtc
    26581 agtgcgctaa tgacagcact tcccgaccat gccgccagtc ccctgatgac ccgcctgcag
    26641 gacgggctgc gcacggaatc tgtccggggt caggcccgtg aacagtttac cggtgcggtg
    26701 gaccggatat ttgacacggc ggagagcgtc tgtgttgcct ctgttgcccg taaggttctg
    26761 cgcgctgttc gtgacaccgt ggtctctgtt gcccgcgcgg tatggaactg gattttcttc
    26821 tgaacccggt ggtgatgcac cagactcttg ttacagattt ttaaccccat gaaaacacaa
    26881 tgcaaacaca gccatacccg ggttgtcccc ttgcgttgtg tttctcaagt taatagaatg
    26941 catcgatcgt tactatcata tcgatcgcta taaacgatcg tttgtggtaa atgacctcct
    27001 ccgtggttgt catgaaaatg cgtctcagga aggttaaata atgcacgaaa agaacatcgc
    27061 cctgctttgt gatgaagccg accgactttt gcaactgaac attaatctgc tccggcaaat
    27121 ggttgatgaa ccggatgtgt tatctgacag taagaacgaa aacagactgc tttttgataa
    27181 acagaaagca ctgaaaagaa ttgaggagct ggagggcgaa caaatcaaaa ccgcccgcag
    27241 ggagatggtg ctggctgttg tcggcacgat gaaagcaggt aaatcaacta ccatcaacgc
    27301 cattgtgggg caggaaattc tgcctaaccg taaccgcccc atgacctctg taccgacgct
    27361 catccgccac gttcccggaa aaactgagcc ggtcctccat ctggaacata ttcagcccgt
    27421 ccgcaattta ttaagcacac tgcaacaaaa actcgacacc ccggcaggac agcaagtcgc
    27481 acagaccctg cagcaaaccg gggatacctg cgaactgctg gatattctgg caaatgatgg
    27541 ctggctcaaa aatgaatacc acggggaaga cgaaatcttt accggactgg catcgttaaa
    27601 cgatctggtt cgccttgctg cggcaatggg gagtgagttt ccttttgatg aatacgcaga
    27661 agtgcagaaa ctgccggtga tcgacgtgga attcagccat ctggtgggga tggatgcatg
    27721 ccagggaaca ctcacactgc tggatacccc cggccctaat gaggccggac aaccgcagat
    27781 ggaagtgatg atgcgggatc aactgcagaa agcctctgcg gtgctggcgg tgatggatta
    27841 cacccagatg aactcaaaag cggatgaaga cgtccgtaaa gagcttaatg ccattgctga
    27901 cgtatcagcc ggccgcctgt ttgtgctggt caataaattt gatgagaaag accgcaatgg
    27961 cgatggggca gatgccgtac gccagaaagt tccggcaatg ctgaacagcg atgtgctgcc
    28021 cgcctcccgc gtttatccag gctcatcacg tcaggcgtac ctggctaacc gtgcgcttca
    28081 tgagttacgg aaaaacagaa cccttcctgt tgatgaagcc tgggtcgatg attttgtcag
    28141 ggaagccttc ggtcgcatga aaaaagaata cgtctgtaaa gacagtgaaa tggcaaccga
    28201 aggggcgaca gacctgtggg aaggctcact tatcgatcaa ctgataacgg aagtcataca
    28261 gagctcccat tccagagcgg cggcactggc ggttgattct gccgccgcaa aactgatgca
    28321 gaatgcggaa aatgtcagcg aatatctgtc attacgccat caggggctac agcaaagcat
    28381 tcagtcactg cagtcgcata tcaccagcct gcttgcggat atccgggaaa tcgcggactg
    28441 tcaggagcag gtgaccgctg atgtcagaat ggccatggag gagatcgatg ccagaacccg
    28501 ggaattactg acgggggtct gcacctcact ggaagaggag ctgaatgact atttcagaag
    28561 cggtaaacgc aaagaacagc aaatgctgga ggaagaaaat tcagcgcagc cccgggagag
    28621 aaacgcattc gctttctttc acgatatttt cggaaccgga aatcagcatg acaggatgcg
    28681 ggactttgat ccggacagcc ccgaaataaa attcagtgat cgcagggaag cccttgaact
    28741 gatgacgcaa atcgaatcga ccgtgaccag cctgcaccgt gaggctgaag cacagttccg
    28801 tcctgaactg gaaaaaatcg tcaggggtat tgaaacaggt tttcgtggta cggccctgta
    28861 cgccacagaa aatattgccg gacgcatcaa tgcccgtctg gaggatgaag gtttcactgt
    28921 aaaaatcagt tttccggcag tcagccagtt acagacccgg ctcgcagtaa aaacaaatct
    28981 gagcgcgctt atggaggaaa gaacggagac agtaacccgt cgctgtcgac aggatggtgt
    29041 ctggggtacg ctttgtcgat gggcaaacac cagtgactgg ggctggaaag agtacagtgt
    29101 ggatgtgagc cgcagcgtga tcaatatcaa caaggtcaga gaggaagtga tgtcactgac
    29161 ccgggcatat ttcggggagc tgcaggcatc cattgagcag gatattaacc agcccgtccg
    29221 ccaggaaatc gatgcctttt tctgtacatt cagggagaaa gttgaacagc tgcgtaacac
    29281 gctgattcag agctctgaag atcataaacg cgatcagcag gcgcaggaac ggctgaccgg
    29341 gcgtcttcag gcgttaaatg aaagggttcc tgagctcatt actgacagta aggcgttaag
    29401 ggaagaactg gagacaatgc tgtgacttca ccatttattc aacaaattgc tgataaccgg
    29461 gtatgtcagg tactcacctg tcttcctgaa aaatttgtgg ttgatttcgc aaacggtatt
    29521 gatgttgcac aggagcatat ccgtacggcc ggagagcgca cgttcttccg gcggttaaaa
    29581 gaggggctga gcggcgaagg tactgcacgg cagaatgcca tcaatgcttc gcttgcacag
    29641 ggcgttgagg cgtccctgcg ctggctgacg gagctgacaa cgtcacttgc caccacaaac
    29701 tacgcgatta cccgggtaaa cgacagggtc agttcactgg tcagtgatac tgccaggctg
    29761 gcgcattatt ccgcagatac gcgggagcag ttactcaccc tggccgatca ggttcaccag
    29821 aaactgaatc atcttgaaga aaaactccac cgtgttgacc tggttcagcg ggcgcagtta
    29881 catcttgagc agatattctc ctggtggagc gccgggcgat atgcgtcatt ttcccctgcc
    29941 ggacgttgtt atgtggcact tgaagagctt cgctggggcg cgtttggtga tgtgatacgt
    30001 cagggcgaaa caggccaggt taaccagcta ctggatattc tcagacataa agcattaacg
    30061 cagatggcac aggagagtgg cggtagtgca accgtgcgtc tgaacactct ggactggctt
    30121 ggtggtcagg gccgggagca ggcagataac gagtggcatg acgcgattaa ctggctgggg
    30181 gactggtgca gtgaagagca gcatccggtg atctggtcaa ccacgcaggc tgcagaacac
    30241 ttaccggttc gcatgccccg tctctgctct gcagaacgcc tctctgaaag tatggtcgat
    30301 gaaatatttc agaagggggc tgcatgagta tagaaataaa aacagggctg gtgctgtccg
    30361 gcggtggggc ggtgggcgct tatcaggcgg gagtggttaa ggcactggca gagtgtggta
    30421 cacagatcag catggtttca ggggccagca ttggcgcatt caatggtgcc attatcgcgg
    30481 cctctcccga tctgtcagaa gctgccgtac gcctggaggc gctctgggat catcttggga
    30541 ataatcaggt gctgtcggta aaccgatcgg tttacttttc attgctgaaa aaattgttcc
    30601 aggcaatgaa cctctgccag atccccggac gtgcaggagc actgcttacg acacttcttc
    30661 gccatatatc gataatcaac ggcttcgaca atccgatgac tcagccgttg ttgtcagatg
    30721 ggcccctgac agcgttgatg gatcattatc ttgatacaga tgctctggca ggcgggctac
    30781 cgctgtatgt gtcgctgtac cccacagaag ggggcatgca ggatattatt gactgcattc
    30841 gtgctgaact gggttccgga accacgaaaa acgccgtttt tcagcatatc cagagtctgc
    30901 cccgcggaca gcagaaagag gctctgcttg cgtcagccgc actgcccctg ttgttccgtc
    30961 cccgtgaggt tcaggggaca atgttcggtg atggtggtat gggaggatgg cgaaatatgc
    31021 agggaaatac ccctgtaacg cctctggtcg atgccggatg caatatggtg attgtgtcgc
    31081 atctgagtga cggttcttta tgggatcgcc aggcttttcc ggacaccaca atccttgaga
    31141 tccgtccccg gaaaaggctg aaatatgcag gtgatggtgg caacagtggc ggtctgctca
    31201 gtttcacgtc ggcacatacc gacgcctggc gtcagcaggg ctacgaggac acgatgctga
    31261 cgatggagca tatccggaaa ccgctggcag cacgtcaggc actgtcccgg tcagaaacgg
    31321 tgttgcagaa aagtctggag ataacggaag aggcagattt ggcactgaga agcgcgatgg
    31381 cccggattaa ataaagacgc cccaaagaaa cgccaccgcc tgtggtggtg tttttcctgt
    31441 ctggtgttgt tgaaaatatt ttcagatata tattacctgg gtgagattta agattaacgc
    31501 atttattaat gtcataagga gagttatcat gaaattcatc gtaaaagcac ttaggtgatg
    31561 ctgccaactt actgatttag tgtatgatgg tgtttttga
//
LOCUS       DAJTQY010000043        31047 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.043, whole genome shotgun sequence.
ACCESSION   DAJTQY010000043 DAJTQY010000000
VERSION     DAJTQY010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31047
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.043"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            389..2047
                     /locus_tag="OO349_002028"
     CDS             389..2047
                     /locus_tag="OO349_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HCR5867098.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(2044..3000)
                     /locus_tag="OO349_002029"
     CDS             complement(2044..3000)
                     /locus_tag="OO349_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HCR5867099.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRCSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            3151..4266
                     /gene="macA"
                     /locus_tag="OO349_002030"
     CDS             3151..4266
                     /gene="macA"
                     /locus_tag="OO349_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HCR5867100.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSLQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            4263..6209
                     /gene="macB"
                     /locus_tag="OO349_002031"
     CDS             4263..6209
                     /gene="macB"
                     /locus_tag="OO349_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HCR5867101.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGISLNIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDIATLDADALAQLRREHFGFIFQRYHLLPHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(6282..6506)
                     /gene="cspD"
                     /locus_tag="OO349_002032"
     CDS             complement(6282..6506)
                     /gene="cspD"
                     /locus_tag="OO349_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HCR5867102.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            6829..7149
                     /gene="clpS"
                     /locus_tag="OO349_002033"
     CDS             6829..7149
                     /gene="clpS"
                     /locus_tag="OO349_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HCR5867103.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            7180..9456
                     /gene="clpA"
                     /locus_tag="OO349_002034"
     CDS             7180..9456
                     /gene="clpA"
                     /locus_tag="OO349_002034"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706768.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HCR5867104.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     KLSDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(9859..9946)
                     /locus_tag="OO349_002035"
     tRNA            complement(9859..9946)
                     /locus_tag="OO349_002035"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9910..9912),aa:Ser,seq:gga)
     gene            complement(10200..10418)
                     /gene="infA"
                     /locus_tag="OO349_002036"
     CDS             complement(10200..10418)
                     /gene="infA"
                     /locus_tag="OO349_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HCR5867105.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(10703..11407)
                     /gene="aat"
                     /locus_tag="OO349_002037"
     CDS             complement(10703..11407)
                     /gene="aat"
                     /locus_tag="OO349_002037"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HCR5867106.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(11449..13170)
                     /gene="cydC"
                     /locus_tag="OO349_002038"
     CDS             complement(11449..13170)
                     /gene="cydC"
                     /locus_tag="OO349_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HCR5867107.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGSVMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRISDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARTLLHDAPLVLLDEPTEGLDAT
                     TESQILELLAKMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(13171..14937)
                     /gene="cydD"
                     /locus_tag="OO349_002039"
     CDS             complement(13171..14937)
                     /gene="cydD"
                     /locus_tag="OO349_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HCR5867108.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELALTDPLTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAAKLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(15060..16025)
                     /gene="trxB"
                     /locus_tag="OO349_002040"
     CDS             complement(15060..16025)
                     /gene="trxB"
                     /locus_tag="OO349_002040"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HCR5867109.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMARMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNA
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            16569..17063
                     /gene="lrp"
                     /locus_tag="OO349_002041"
     CDS             16569..17063
                     /gene="lrp"
                     /locus_tag="OO349_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HCR5867110.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            17198..21226
                     /gene="ftsK"
                     /locus_tag="OO349_002042"
     CDS             17198..21226
                     /gene="ftsK"
                     /locus_tag="OO349_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCR5867111.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAPEQPVAGNAWQAEEQQSTFAPQSTYQ
                     TEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYYFEEVEEKRARERE
                     QLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVEAAAAVSPLASGVKKATLATGAAA
                     TVAAPVFSLANGGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYGIKLPSQRAAEEKA
                     REAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQHDVPVNAEDADAAA
                     EAELARQFVQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGPHEPLFTPIVEPVQ
                     QPQQPVAPQQQYQQPQQPVAPQPQYQQPQQQVAPQPQYQQPQQPVAPQQQYQQPQQPV
                     APQPQYQQPQQPVAPQPQYQQPQQPVAPQPQDTLLHPLLMRNGDSRPLHKPTTPLPSL
                     DLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGPVITRFELNLAPGV
                     KAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTVYLREVLDNAKFRD
                     NPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAMILSMLYKAQPEDV
                     RFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMERRYKLMSALGVRNL
                     AGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLVDEFADLMMTVGKK
                     VEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAFTVSSKIDSRTILD
                     QAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKARGRPQYVDGITSD
                     SESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIGYNRAARIIEQMEA
                     QGIVSEQGHNGNREVLAPPPFD"
     gene            21381..21992
                     /gene="lolA"
                     /locus_tag="OO349_002043"
     CDS             21381..21992
                     /gene="lolA"
                     /locus_tag="OO349_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HCR5867112.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            22003..23346
                     /gene="rarA"
                     /locus_tag="OO349_002044"
                     /gene_synonym="mgsA"
     CDS             22003..23346
                     /gene="rarA"
                     /locus_tag="OO349_002044"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HCR5867113.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDDTRRAIAELVNGDARRALNTLEMM
                     ADMAEVYDSGKRVLKPELLIEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            23437..24729
                     /gene="serS"
                     /locus_tag="OO349_002045"
     CDS             23437..24729
                     /gene="serS"
                     /locus_tag="OO349_002045"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HCR5867114.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMIAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            24969..27413
                     /gene="dmsA"
                     /locus_tag="OO349_002046"
                     /pseudo
     CDS             24969..27413
                     /gene="dmsA"
                     /locus_tag="OO349_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
     gene            27424..28041
                     /gene="dmsB"
                     /locus_tag="OO349_002047"
     CDS             27424..28041
                     /gene="dmsB"
                     /locus_tag="OO349_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HCR5867115.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            28043..28834
                     /locus_tag="OO349_002048"
     CDS             28043..28834
                     /locus_tag="OO349_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HCR5867116.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGWAMRLL
                     PAISVLALVVSGVMSVMQGTELATIHSSVQQAAALVPDYGALMSWRIVLLAVALCLWI
                     APQLKGYQPAVPLLSVSFILLLAGELIGRGVFYGLHMTVGMAVAS"
     gene            complement(28870..29496)
                     /locus_tag="OO349_002049"
     CDS             complement(28870..29496)
                     /locus_tag="OO349_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR5867117.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVLELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            29811..30959
                     /locus_tag="OO349_002050"
     CDS             29811..30959
                     /locus_tag="OO349_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5867118.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLSSFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFSAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
BASE COUNT         7653 a         7616 c         8351 g         7427 t
ORIGIN      
        1 tggtcgtttt atagggtcgt cgtaaaaaat gacgacggaa ataatgcgcg gctattttaa
       61 aaacgaaggc gagtcattca ccagataaat aaatccagta aatttgattt agggcaacag
      121 cgggttgccc catatagtca tttgtctgat tgacagtgta gtgcaagcaa aagatttaat
      181 cctttaggcg taataaaaaa taatttatca tgctaattat ttgattttgt tgtttttgcg
      241 ggcttatcag caagagggag tataacgcga ttattcgctc atttttcaga caattgccat
      301 gcttaaatgt gatgtcatca cgtattagca aggcctttcc cgttatactg cgagcgtaaa
      361 ggataagtca catatttctg gaggggatat gattcttgag cgcgttgaaa ttgtgggttt
      421 tcgcggtatc aaccgtttgt cgttgatgct cgaacaaaac aacgtcctga ttggggagaa
      481 cgcgtggggt aaatccagct tgctggacgc cttaaccctg ctgctatcgc cagaatcaga
      541 tctctaccat tttgagcgcg acgatttctg gttcccgccg ggagatatca acgggcgaga
      601 acatcatctg catattattt tgaccttccg cgaatcgctg ccaggccgac atcgggttcg
      661 ccgttatcgg ccgctggaag cgtgctggac gccatgcacc gatggctatc accgtatttt
      721 ttatcgtctg gaaggggaga gtgcggaaga cggcagcgtg atgacactgc gcagttttct
      781 cgataaagac ggacatccga ttgatgttga ggatattaac gatcaggcac gccatctggt
      841 gcgtttaatg ccggtgctgc gcttgcgtga tgcccgtttt atgcgccgta ttcgtaacgg
      901 cacggtgcca aatgtcccca atgtggaagt caccgcgcgc cagctcgatt tcctcgcccg
      961 tgagttgtcc tcacatccgc aaaatctctc tgatggacag attcgtcagg gactttccgc
     1021 aatggtgcag ttgcttgagc attatttctc tgagcagggg gctggacagg cgcgatatcg
     1081 tttaatgcgg cggcgggcca gcaatgagca acgaagctgg cgctatctgg acatcatcaa
     1141 ccggatgatt gaccgacctg gagggcgctc gtatcgggtt attttgctcg gcctgtttgc
     1201 tactttattg caggcaaaag gcacattgcg actggataaa gacgcgcgtc cgttattgct
     1261 aattgaagat ccagaaaccc gcctgcatcc gattatgctt tccgttgcct ggcatctgtt
     1321 gaatcttttg cctttacagc gtattgctac caccaactct ggcgagctgc tttcgttaac
     1381 tccagtggaa catgtttgcc gcctggtgcg tgaatcttct cgtgttgccg cctggcgtct
     1441 ggggccaagt ggcttgagca ccgaagacag ccgccgcatc tcttttcaca ttcgttttaa
     1501 tcgtccgtca tcgctgtttg ctcgctgctg gttgctggtg gaaggggaaa cggaaacctg
     1561 ggttatcaat gaactggcgc gtcagtgcgg acatcatttt gatgccgaag ggatcaaggt
     1621 cattgagttt gcccagtccg ggctaaagcc actggttaaa tttgcccgcc gaatggggat
     1681 tgaatggcat gtactggtcg atggcgatga agcagggaag aaatatgccg ctacggtacg
     1741 cagcctgttg aataacgatc gggaagccga acgagaacat ttaacggcgt taccggcgct
     1801 ggatatggaa cattttatgt atcgccaggg attttccgat gtgttccacc gcgtggcgca
     1861 aatcccggaa aatgtaccga tgaatctacg caaaattatc tcgaaagcga tccatcgctc
     1921 ttccaaaccc gatcttgcca ttgaagtggc aatggaggca ggacgtcgtg gtgtggactc
     1981 cgtaccaacg ctgctgaaaa aaatgttctc acgcgtgctg tggctggcgc gcggtcgcgc
     2041 ggattaaccg cgaaacatcg tggccatttg tggctgaata gcgtcgagca tctcatagcg
     2101 ccgacggtat tcagcccgtt ttttactggc gatttcggca atctcttttc gtgctatctg
     2161 tgctggaagg cggtaatggc gttcagcatc acatacgccg ccaaccgact cccagaacgc
     2221 gttgtaatca gcatggatct tgccttcttt atcgcgataa cgcaggctgc ggtaaatatg
     2281 cgtttcattg ctgacggcaa taatctgctc tacctgcaaa cgttgggcaa acagacaggc
     2341 cgcttccatc acgaggcgtt tgggaaatag cccgtggcag gctttcgtcg cattctggat
     2401 ttcctgatgt ggaatttccc attttgcgcc ttgcagtccg ccaataaaca tcgttctttt
     2461 cccctgatat tcacacaggg taaacgtgat ctctgccaga ggaatacctt cgctgttgcg
     2521 gaacaggatt gtgctgtcac cttctttatc cattgagatc atcatggtca gctcaagcgt
     2581 gaactgctca ccgtttttgc cttccagttt agccagttgc agcccggggg tattcaaata
     2641 taagctgaat tcttccgccg acatacaccc gcggagtaac gcataatggt aacgtaacgc
     2701 ctccagcaat tgcttacggc taagattcgc ggcaaggtaa gggcgatgca gacgcacagg
     2761 cagtcgcggc tggcgcgtta acaacacatt gagattaggc cagtgggaaa gttcgttcat
     2821 ccactcaacg cttaaacgcg gcataatcaa cgagcgcagc aaaaatttct ggcgaaaact
     2881 acagcgatgc cagaatttac ccggccgaca ctgtccacgt gccagactaa gaaaaagtga
     2941 caggctgctg agagattcag atggcgtaaa ggtccgttca gttagctgcg acatattcat
     3001 gaaatcaatg gttatacatg acgtcgattt caccattgcg tatcttaacc aaacatcaat
     3061 agtgtgatta ctaacgtaaa ttttagggtt ttgttgatat ttcgttgaag ttaatgaccc
     3121 ggattggcat atggagtatt cagaatattt atgaaaaagc ggaaaaccgt gaagaagcgt
     3181 tacgttattg cgctggtgat agtcatcgcc ggactgatta cgttatggag aattcttaac
     3241 gcacccgtgc cgacttatca gacactgatt gtgcgccccg gtgatttaca gcaaagcgtg
     3301 ctggcgaccg gaaagctgga cgcgctgcgt aaggttgacg tgggcgcgca ggtcagcggt
     3361 cagttgaaaa cgctgtcggt ggcgattggc gataaagtaa aaaaagacca gcttttaggg
     3421 gtcattgatc ctgaacaggc tgaaaaccag atcaaggagg tcgaagcaac gctgatggag
     3481 ctacgcgcgc agcggcagca ggcggaagcg gagctgaaac tggcgcgggt gacgtattcc
     3541 cgtcagcaac gtctggcaca aacgcaagcg gtttcactgc aggatctcga taccgccgcg
     3601 acggagatgg ctgtgaaaca ggcgcaaatt ggcaccattg acgcgcaaat caagcgcaat
     3661 caggcctctc tcgatacggc taaaaccaat ctcgattaca cccgtatcgt tgccccgatg
     3721 gctggggaag tgacgcaaat caccactctg caaggccaga cggtgattgc cgcacagcaa
     3781 gcaccgaaca ttctgacgct ggcagatatg agcactatgc tggtaaaagc gcaggtttct
     3841 gaagcggatg taatccacct gaagccgggg caaaaagcct ggtttacggt gcttggcgat
     3901 ccactgacgc gctacgaggg gcaaatcaag gatgtgctac cgacgccgga aaaggtaaac
     3961 gacgctattt tctattacgc ccgttttgaa gtacccaacc ccaatggttt gctgcggctg
     4021 gatatgaccg cgcaagtgca tattcagctc accgatgtga aaaatgtgtt gacgatccct
     4081 ctgtcggcgt taggcgatcc ggttggcgat aatcgttata aagtcaaatt gttgcgtaat
     4141 ggtgaaacac gcgagcgtga agtgacgatt ggcgcacgta acgataccga tgttgagatt
     4201 gtcaaagggc tggaagcggg cgatgaagtg gtgattggtg aggccaaacc aggagctgca
     4261 caatgacgcc tttgctcgaa ttaaaggata ttcgtcgcag ctatcctgcc ggtgatgagc
     4321 aggttgaggt gctgaagggc atcagcctca atatttatgc gggtgagatg gtcgcgattg
     4381 ttggcgcttc gggttccggt aaatcgaccc tgatgaatat tctcggctgc ctggataagg
     4441 ccaccagcgg cacctatcgc gtagccggtc aggatattgc cacgctggac gccgatgcgc
     4501 tggcgcaact gcgccgcgag catttcggct ttattttcca gcgttaccat ttgcttccgc
     4561 atttaaccgc cgagcagaac gttgaagtac ccgccgtcta tgccggtctt gagcggaaac
     4621 agcgactgct tcgtgcccag gagttgctgc aacggctggg gctggaagac cgtacagagt
     4681 attatccggc acagctttcg ggtggtcagc aacagcgcgt cagcatcgcg cgggcattga
     4741 tgaacggcgg tcaggtaatt cttgccgatg aaccgaccgg cgcactggac agccattctg
     4801 gcgaagaggt gatggcgatc ctgcatcagc tgcgcgatcg cgggcatacg gtgattatcg
     4861 tcacccacga tccgcaggtc gctgctcagg ccgagcgggt gatcgaaatt cgcgacggcg
     4921 aaattgtgcg caatcctccc gccattgaaa aagtgaatgt tgctggcggg acggagcctg
     4981 ttgtcaatac agtgtctggc tggcggcagt ttgtcagcgg ttttaacgag gcgctgacga
     5041 tggcatggcg ggcgctggca gcgaataaaa tgcgtacttt actgaccatg ctggggatta
     5101 ttatcggtat tgcgtcggtg gtttccattg tcgtggtggg tgacgccgcc aaacaaatgg
     5161 tgctggcgga tattcgttct attggtacga atactattga tgtctatccc gggaaagatt
     5221 ttggcgatga cgatccgcaa tatcagcagg cgctgaagta cgacgactta atcgccatcc
     5281 aaaaacaacc gtgggtcgcc tcagccacac ctgccgtctc gcaaaacctg cgcctgcgtt
     5341 ataacaatgt tgatgttgct gccagtgcca atggcgtgag cggcgattat tttaatgtct
     5401 atggcatgac cttcagtgaa ggaaacacct ttaatcagga gcagctgaac ggtcgtgcgc
     5461 aggtcgtggt tctcgacagt aatactcgcc gccagctttt cccccataaa gcagatgtgg
     5521 ttggcgaggt gattctggtc ggcaatatgc ccgccagagt cattggtgtg gcggaagaaa
     5581 aacagtcgat gtttggtagc agtaaagtgc tgcgtgtctg gctaccttac agcacgatgt
     5641 ccgggcgagt tatgggccag tcgtggctta actccattac tgtcagggtg aaagaaggat
     5701 ttgacagcgc cgaggcggaa cagcaactca cgcgtttact ttcactgcgc cacggtaaga
     5761 aggatttctt tacctggaac atggacggcg tcttgaaaac tgttgaaaag accacacgta
     5821 ctttacaact gtttctgacg ctggtggcgg tgatttcgct ggtggtgggc ggtattggtg
     5881 taatgaatat tatgctggtg tcagtgaccg agcggacgcg ggaaattggc attcgcatgg
     5941 ctgtaggtgc gcgagcaagc gatgttttgc aacagttcct gatcgaagcc gtactggttt
     6001 gcctggtcgg tggcgcgttg ggaataacac tgtcactgtt aattgctttc accttgcagc
     6061 ttttcttacc cggctgggag attggttttt caccgttggc gctgctgctg gcgtttctct
     6121 gctcgacggc caccgggatt ttatttggct ggttacccgc acgaaatgcg gcacgactgg
     6181 atccagtaga tgctctggca cgggagtaat ttttgagata aaaatgccag ccgatcgggc
     6241 tggcattttg cctttaggat gtacacaatg agacagaaga gctatgcgac tgccgcttct
     6301 acttcgacgg gcacaataac actggcgtga ttgccttttg gcccctggtg gacatcaaac
     6361 tgaacggatt gtccagcttt tagcgttctg taaccatcca tctgaatggt ggaataatga
     6421 gcgaaaatat cttcgccgcc gccttcaggg cagatgaaac caaacccttt ggcattgttg
     6481 aaccacttaa cagtaccctt ttccatgctt cgacatcctt cgcaaatctt atacaagtaa
     6541 gatggaataa accggggtca gagagggggc tgttcaaaac ctcgccaact ctagaaatac
     6601 aatttagaga attagggcga gccgtcaagc atttgacagg ggccaggggg caggtatgaa
     6661 tcaaaaattt gaagcagtta acgctattga caggaatgtg acagatgtcg ctgatgccaa
     6721 cgatagatga tagttatcta tcatgtggag tagattggtc aggcaaataa gctcttgtca
     6781 gcggcagggc gttctgccga taaccgtaac cgaagatgat aactgacaat gggtaaaacg
     6841 aacgactggc tggactttga tcaactggcg gaagaaaaag ttcgcgacgc gctaaaaccg
     6901 ccatctatgt ataaagtgat attagtcaat gatgattaca ctccgatgga gtttgttatt
     6961 gacgtgttac aaaaattctt ttcttatgat gtagaacgtg caacgcaatt gatgctcgct
     7021 gttcactacc aggggaaggc catttgcgga gtctttaccg ccgaggttgc agaaaccaaa
     7081 gtggcgatgg tgaacaagta cgcgagggag aatgagcatc cattgctgtg tacgctagaa
     7141 aaagcctgaa tgcaggcata aaaattgggg gaggtgccta tgctcaatca agaactggaa
     7201 ctcagtttaa atatggcttt cgccagagcg cgcgagcacc gtcatgagtt tatgaccgtc
     7261 gagcacttgt tactggcgct gctcagtaac ccatctgccc gggaggcgct ggaagcgtgt
     7321 tctgtggatt tggttgcgct ccgtcaggaa ctggaagcct ttattgaaca aaccacaccc
     7381 gttctgcctg ccagtgaaga ggagcgcgac acacagccga cgctgagttt tcagcgtgta
     7441 ctgcaacgtg cggtcttcca tgtccagtcc tccggtcgca atgaggttac cggtgcaaac
     7501 gttctggtcg ctatcttcag cgaacaggag tcgcaggcgg catatctgtt gcgtaaacat
     7561 gaagtcagcc gtctcgatgt ggtgaacttt atctctcatg gcacgcgtaa agacgagccg
     7621 acacagtctt ctgatcctgg cagccagcca aacagcgaag aacaagctgg tggggaggaa
     7681 cgtatggaga atttcacgac gaacctgaac cagcttgcgc gtgtgggcgg aatcgaccca
     7741 ctaattggtc gtgagaaaga gctggagcgt gctattcagg ttctctgccg tcgccgtaaa
     7801 aacaacccgc tgctggtggg ggaatctggt gtcggtaaaa ccgcgattgc agaaggtctt
     7861 gcctggcgaa ttgttcaggg tgatgtgccg gaagtgatgg ctgactgtac gatttactct
     7921 ctcgatatcg gttctctgtt agcgggcaca aaatatcgcg gcgactttga aaaacgtttt
     7981 aaagcgttgc tcaagcagct ggagcaggac actaacagca tcctgtttat tgatgagatc
     8041 cacaccatta tcggtgcggg tgcagcgtct ggtggtcagg tcgatgcggc taacctaatc
     8101 aaaccgttgc tctccagcgg taaaattcgc gtaattggtt cgacaaccta tcaggagttc
     8161 agcaacattt tcgagaaaga ccgtgctctg gcgcgtcgct tccagaaaat tgatattact
     8221 gaaccgtcga tcgaagaaac tgttcaaatc atcaatggcc tgaaaccgaa gtatgaagcg
     8281 caccacgacg tgcgttatac cgcaaaagcg gtgcgtgcgg cggtagagct ggcggtgaaa
     8341 tacattaacg atcgtcatct gccggataaa gccattgatg ttatcgacga agcgggcgct
     8401 cgcgcacgtc tgatgccggt aagcaaacgc aagaaaaccg ttaatgtggc ggatattgag
     8461 tccgtggtgg cccgtattgc acgcattcca gagaagagtg tttctcagag tgaccgcgat
     8521 accctgaaaa aactcagcga tcgcctgaaa atgctggtct tcggccagga taaagccatt
     8581 gaggcgctga ctgaagccat taagatggcg cgtgcaggtt taggtcacga acataaaccg
     8641 gtcggttcgt tcctgtttgc cggtcctacc ggggtcggga aaacagaggt gacggtacag
     8701 ctttcgaaag cgttgggcat tgagcttctg cgctttgata tgtccgagta tatggaacgc
     8761 cataccgtca gccgtctgat tggtgcgcct ccgggatacg ttggttttga tcagggcggt
     8821 ttgctgaccg atgcggtaat caagcatcca catgcggtgc tgctgctgga cgaaatcgag
     8881 aaagcgcatc cggacgtgtt caatattctg ttgcaggtga tggacaacgg tacgctgacc
     8941 gataacaacg gacgcaaagc ggacttccgt aacgtggtgc tggtgatgac cactaacgcc
     9001 ggggtacggg aaactgagcg caaatccatt ggtcttatcc accaggataa cagcaccgat
     9061 gcgatggaag agatcaagaa gatctttaca ccggaattcc gtaaccgtct cgacaacatt
     9121 atctggttcg atcatctgtc aactgacgtg atccatcagg tggtggataa attcatcgtc
     9181 gagttgcagg ttcagctgga tcagaaaggt gtttctctgg aagtgagcca ggaagcgcgt
     9241 aactggctgg ccgagaaagg ttacgaccgg gcaatgggcg cacgtccgat ggcgcgtgtc
     9301 atccaggaca acctgaaaaa accgctcgcc aacgaactgc tgtttggttc gctggtggac
     9361 ggcggtcagg tgacggttgc gctggataaa gagaaaaatg agctgactta cggattccag
     9421 agtgcacaaa agcacaaggc ggaagcagcg cattaatctg attgtcaggt aggttggtga
     9481 agtccgtaat ctcgtcaggg gttacggact ttttatttat ggggtggaac ggtgaaaacc
     9541 ctatttttgg aggtgaaggt aagttgttga taattagtgc tgctggaagg taaggataaa
     9601 aaagggtgct gcaggagaat gggatggttt tgctttatta acaacgggct aaacgtgtag
     9661 tatttgagtt cactgccgta caggcagctt agaaatcgac ggggtgcggt aaaacctttg
     9721 cgaacgcgtt cactgccgta caggcagctt agaaattcac aggtaacata ctccacccgc
     9781 ccaccatgtt cactgccgta cagacagata aaatgcgaaa aaaaagctcg cactttcgta
     9841 cgagctcttc tttaaatatg gcggtgaggg ggggattcga acccccgata cgttgccgta
     9901 tacacacttt ccaggcgtgc tccttcagcc actcggacac ctcaccaaat tgttttgcta
     9961 ccaaacctca tgggtggcaa cggggcgcta ctatagggag ttggagtaaa acggtcaaga
    10021 agaattttaa tgataattat tgtttgctca tactgtaaac aacttgtgca gtatatctac
    10081 atcgagacaa gttatggact tatacttcca aagtacttca tacatatcac aaaataaaaa
    10141 ggccggttaa accgaccttt tactcgttct ttctcttcgc ccatcaggcg gtaaaacaat
    10201 cagcgactac ggaagacaat gcggcctttg ctcaggtcgt acggggtcag ttcaacagtc
    10261 actttgtcgc ccgtcaggat gcggatgtag tttttgcgca ttttaccgga gatgtgtgca
    10321 gtaaccacgt gaccgttttc taactctacg cggaacatgg tattaggcaa cgtttcaaga
    10381 acggtacctt gcatttcaat attgtcttct ttggccatct aatcctctgg ggtatcacta
    10441 ccgtaatttg aaccggcaag ataatgccga agttctgtaa ataagtaaag atttgcgcgc
    10501 taaatcgcaa caaacaggtt cggcacatta ctccgaaaac acacggctaa gccgcaccaa
    10561 aagcgcaacg tataagggag cggtgagata aacgatgggc gttacctgac gcgaaaaatt
    10621 ccttatcggc agcggggtaa tgagcgtaac caactctgcg accgcaatta taacactctg
    10681 gggagaaatg tgccgaaaac attcattctt gtggtgaaaa caagcaccgt ggtacccaga
    10741 aattattcgg caatcgtccg aggcgcattt gattgagata attaaggtaa tcccggcggg
    10801 gaatttcgca ggcaccaagc gatgctgtgt gatcgttaag gacctggcag tcgataagct
    10861 taccgccatg accgataaat tcctcacaga ataccagaag cgccgtttta gacgcatttt
    10921 ccatccggct gaacatggac tcgccacaaa atagcgttcc ctgggccacg ccgtacatac
    10981 cgccgacaag ctcatcttca cgccagactt caatggagtg ggcatgaccg agttcgtgaa
    11041 ggcgatggta ggcttcgacc acgccacgcg tgatccaggt tccttcttcg cgatcgctgg
    11101 cacagccttc aatgacctga ccaaaagcgt aattcatcgt gacacgatag ggcgagcgtt
    11161 tatgaaatcg cttcatacta cggctgatat gcagtgattc tggccatagc accgcgcggg
    11221 gatcgggcga ccaccagagg atggggtcgc ctggagaaaa ccacggaaaa ataccacgct
    11281 ggtaagccat taacaggcgc gcagggctaa gatcgccccc aagtgccagc aggccgttag
    11341 gctcacgtaa tgcgccttcc ggggaaggga aggctattga atggcgagaa agctgaacca
    11401 agcgcatgac cgcaaaactc cacgcaagtc ggatcgttca ataatagctt acaaaccctg
    11461 cttgaactgg taataacgcc cctgtctggc aagcagttct gcgtgagtac cttgctcaat
    11521 aatttgcccg ttgtccatca ctattatttg ttggaaacga gagagtccgc gaagtcgatg
    11581 ggtgaccatt aacaccgttt tctcacgcat catttttgca agcaattcaa ggatctggct
    11641 ttcggttgtg gcatctaagc cttcggtagg ttcatccagc aacaccagtg gcgcatcatg
    11701 taacagcgta cgggcgatag ccagacggcg cagttcacca ccggagagct ggcgtccgcc
    11761 ttcacctaac caactgttga gacctgcatc ctcgagcagc ttctccagac caacgcgacg
    11821 caagatctcc gacagagcct catcactact gccaggcgag gcgagtaaca gattatcacg
    11881 cagcgtggcg ctaaacagat gcactcgctg aggaacaacg ctgatggtct gtcgtagagc
    11941 cgcttcattc aggctggcta tggggctatc gttaagcaaa atctcgccct gttgcgggtc
    12001 ccatgcgcgg gtcagcagtt gtaacagtgt tgatttgccg catccggttc gcccgagaat
    12061 cgctatatgt tccccggcgt ttacctgaag agaaatccct ttaagtgcct gttgggattg
    12121 ctccggataa gtgaactgaa catcccgtaa tgtcagcgaa acgcgatcgg caacacgagt
    12181 ttgggtatca gggaaggtga cctccggttt ttgatccgtt aagtcagaga tacgtacggc
    12241 agaggcaatg acttgcccca gatgctgaaa tgcacccgtt actggtgcca gtgcttcaaa
    12301 cgcggctaac gcacaaaaga caaacagggc aattaatgcg ccgggttgag cattgccgcc
    12361 aacgccgcca gacgccatcc acagcatcag gatcaccgct aacgcgccaa tgagcagcat
    12421 tatcgcttgc gacaatgcgg tcagttcaga ttgacggcgt tgcgcttcca gccattgaat
    12481 ttctgtattc tctagttgcg tgcgataacg atcgctggca ccaaaaatgg tcagctcagc
    12541 ttgcccttgc agccaggccg tcagttgttg gcgatactgt ccgcgaagat gagtcagatt
    12601 ttgcccggtg ctttttcccg cacgataaaa cagcggtggc atcaggaaaa gcgtcagtaa
    12661 cataacgctg cccagcgtaa aggcgagggt gaaatcaagg aaacttaacc cgattgtcac
    12721 caccataatc accacaaaag cgcccaccag cggcgagata acgcgcaggt aaagatgatc
    12781 gagcgtatca acatccgcca ccacgcggtt gagcaattcg ccctgacgat agcgcgccag
    12841 tccggcaggg gagaggggca acaatttgct gaaggtgtaa atgcgcagat gctgcaacac
    12901 gcggaaagtc gcgtcgtgac ttaccagacg ttcaaaataa cgcccggcag tacgggtgat
    12961 tgcggcacca cgcacgcccg cagcgggtag catatagttg aagctgtaca gtccggcaac
    13021 ccccgcaacc gctgaggccg agaggaacca gccggaaagt gtcaacagac cgatactggc
    13081 gagcagcgtc acaattgcca gcacaatacc aagacttaac atccatttat gacgtttata
    13141 cagtgccaga tagggtagca aagcgcgcat ttaaatctcc tcctgacgat gggccagtaa
    13201 tgtggcgaat gggccaccag ccacacttaa ttccgcgtaa cgtccttgct caataatccg
    13261 gccatcctgc ataacccaaa tgacatccca gtcagcaaga tcttctaact ggtgggtgac
    13321 cattaacgtt gtctggcgca gagaggcggc attcagcgcc tccattacgc gctgttcact
    13381 gtgagcatca aggctggcag cgggttcatc caacagtaat agcgaacagg gatttagtaa
    13441 cgcacgggcc accgccacgc gctgcgcctg ccccacggaa aggcgggcag cctggtcgcc
    13501 aacaggtgta tcaacgcctt gtggcaggag cggtagaaac tcgctgaccc aggcgttatc
    13561 cagcgctgct tgtaattctt gttcgctggc atcaggtcgc gccagtagta cgttatcccg
    13621 caattttgct gccggtaatt gtgggttttg cccaacccag gagagatgtt tacgccatga
    13681 ttctggtgat aaatcacgta attctatccc gttgattcgt aatgatccct gatatgagag
    13741 aaaaccagaa agcgcgttca gcagtgagct tttacctgaa ccgctgcggc caaccaacac
    13801 tgcacgttgg cctgctggca aagtaaagtt cagcggtccg gccagcgttt taccttccgg
    13861 cgacgtgata aacagatcct cggcctcaat ggtcagcgga tcggtcaatg ctaattccgc
    13921 ctcaccgcgc tgcggatggg cgagcggggt ttccataaac gttttcagac tgtcagctgc
    13981 accaacagcc tgggctttag catgataaaa cgtaccgaga tcgcgtaatg gctggaaaaa
    14041 ctctggcgca aggatcaggg ccagaaaacc tgcagccagc gtcaccccag tatcgtagtg
    14101 accaaaatcc agctcgccga gataggaaaa accaaagtag accgccacca gagcaattga
    14161 cagcgaggta aaaaattcga gaatgccgga ggataaaaac gccagtcgta gcacttccat
    14221 tgtccgttgg cggaaatctt ccgaagcaga acgaatactt tcaatttcag cttcaccacg
    14281 accaaaaata cgcaatgttt ccatgccgcg caggcgatcg aggaaatgcc cacttaagcg
    14341 agcaagagcg agaaagttac gccggttagc atcggcagcc cccattccaa ccagcgccat
    14401 aaacaacgga attagcggtg ctgtgcccag cagaatgagc gccgcagccc agttagaggg
    14461 gaagatcgcc accacaatca gcaacggcac cgacactgcc agcgccattt gcggcagata
    14521 gcgtgcatag taatcatgca tatcgtcaat ttgctcgagt accagcgtcg cccagctccc
    14581 cgcaggttta ccctgaatcc acgctggccc tgcttgttgc agacggtcga gaacctgacg
    14641 gcggatggca aagcggatat gctgcccggc gtgataaccc acccgttcgc gtaaccagac
    14701 cacccatgcg cgcaatacaa aggtcagaac cagtaacgta aagggaagca gcagggcttc
    14761 acggggaata ttctccataa tcatatgttg cagaattcgc gccatgaacc aggcctgggc
    14821 aatgatcaat atgccgctta caaagcccag cagacgagaa atattcagcc aacgttggga
    14881 gatgacgctt tgctgtttta accagcgggt taactctttt tgacgagatt tattcattgc
    14941 acgcttagca ggtgagttat cagaattatt tgcagagcaa tgttacaacg gggaaaaaat
    15001 aaaggcgacc catagtcgca aggtgtcgcc ttctttactt ttgttactga tttgtaaaat
    15061 tattttgcgt cagctaaacc atcgaggtag cgttccgcat caagtgctgc catgcagcca
    15121 gtaccggccg aagtaatggc ctggcgataa atgtgatcca tcacgtcgcc tgcggcaaag
    15181 acgccaggaa tgctggtctg ggtggcatta ccatgaatac ccgactgtac tttgatgtag
    15241 ccgttttcca gttccagctg cccttcgaaa atcgcagtat tcgggctgtg accgatagca
    15301 acaaacagac cggcaacgtc gagtgactcg atgttatcgc tgttttgcgt atcgcgcaga
    15361 cgaacgccag tgacacccat ttgatcgccg gtcacttctt ccagcgtacg gttggtgtgc
    15421 agaatgatgt tgccgttctc cactttatcc atcagacgct taataaggat tttttccgcg
    15481 cggaaaccgt cacggcggtg aatcagatgc acttccgaag cgatgttaga cagatacagc
    15541 gcctcttcaa ccgcggtatt gccgccgccg atgaccgcaa ctttctggtt gcgatagaag
    15601 aaaccgtcgc aggttgcaca agcagaaacc ccacggcctt taaaggcttc ttcagagggc
    15661 aggccgagat agcgtgcaga agctccggtg gcaataatca gcgcgtcgca agtgtattcg
    15721 gcgttatcgc cattcagacg gaacggacgg ttttgcagat ccaccttgtt gatatgatca
    15781 aaaatgatct cagtttcaaa cttggtggca tgttcgtgca tgcgcgccat taataacgga
    15841 ccggtcagat cgtttggatc accaggccag ttttccactt ccgtggtggt ggtcagttgg
    15901 ccgccttttt ccatgccggt aatcagcaca ggttgcaggt tggcgcgcgc cgcgtagaca
    15961 gcagcggtgt atcccgccgg gcctgaaccc aggataagca gtttactgtg tttggtcgtg
    16021 cccatgagat ccccatagtt gttggcagac aatgggcagg attgtaggga atttacagac
    16081 gtaaaaaaag agtatgacga ttttgttaac aatttgtgca atcggcagca tcgataagca
    16141 ggtcaaattc tcccgtcatt atcacctctg ctacttaaat ttcccgcttt ataagccgat
    16201 taaatgatga ataaacgccc ttgttaatga atatctggca tgttgtacta aaaatcgatg
    16261 ttttgctttg acaatcccct agtgttttgc gaaaacattc gaggaagaaa aaaacagtat
    16321 tcttatatgc gcataaccat gtatgtaaat accatgttta ccgtgctagt gaaatctacg
    16381 tatggcgtgg acagacgcca ttcgtgatgt cgatagctgc cacaaggcaa cggtcttctc
    16441 accgtagacc caggcattgc gcgccgtgaa tcttcatgat ttcggtctat cgtgacgggt
    16501 agcgactctg aacagtgatg tttcagggtc agacaggagt agggaaggaa tacagagaga
    16561 caataataat ggtagatagc aagaagcgcc ctggcaaaga tctcgaccgt atcgatcgta
    16621 acattcttaa tgagttgcaa aaggatgggc gtatttctaa cgtcgagctt tctaaacgtg
    16681 tgggactttc cccaacgccg tgccttgagc gtgtgcgtcg gctggaaaga caagggttta
    16741 ttcagggcta tacggcgctg cttaaccccc attatctgga tgcatcactt ctggtattcg
    16801 ttgagattac tctgaatcgt ggcgcaccgg atgtgtttga acaattcaat accgctgtac
    16861 aaaaacttga agaaattcag gagtgtcatt tagtatctgg tgatttcgac tacctgttga
    16921 aaacacgcgt gccggatatg tcagcttacc gtaagttgct gggggaaacc ctgctgcgtc
    16981 tgcctggcgt caatgacaca cggacatacg ttgttatgga agaagtcaag cagagtaatc
    17041 gtctggttat taagacgcgc taacacggaa caggtgcaaa atcggcgtat tttgattaca
    17101 ctcctgttaa tccatacagc aacagtactg gggtaacctg gtactgttgt ccgttttagc
    17161 atcgggcagg aaaagcctgt aacctggaga gcctttcttg agccaggaat acactgaaga
    17221 caaagaagtc acattgacaa agttaagtag cggccgccgc cttctggagg cattgctgat
    17281 ccttattgtc ctgtttgccg tctggttgat ggctgcctta ctaagcttta acccttcgga
    17341 ccccagctgg tcgcaaacgg cctggcatga acctatccat aatttaggtg ggatgcccgg
    17401 tgcgtggttg gcagatacgc tgttctttat ttttggcgtg atggcttaca ccattcccgt
    17461 cattattgtc ggcggttgtt ggtttgcctg gcgtcatcag tccagcgacg aatacattga
    17521 ttattttgcc gtttcgctac gcatcattgg cgttttggcg ctcatcctta cctcctgtgg
    17581 tctggcggca atcaacgctg acgatatctg gtattttgcc tccggtggcg tcattggcag
    17641 cttactaagc actacgctac aaccactgct acacagtagc gggggaacta ttgcgctgct
    17701 ctgcgtttgg gcagcgggcc tgacgttgtt caccggttgg tcatgggtga ccattgctga
    17761 aaaactcggc ggctggattt taaacattct caccttcgcc agtaatcgta cccgtcgcga
    17821 tgatacctgg gtcgatgaag atgagtatga agacgacgaa gagtatgaag atgaaaatca
    17881 cggcaaacag catgaatcac gccgtgcccg tattcttcgc ggcgcgctag cgcgtcgtaa
    17941 acggttggcg gaaaaattca ttaatccgat ggggcggcaa acagacgctg cgttgttctc
    18001 cggtaagcgg atggatgatg acgaagagat tacctacact gcacgcggtg tggctgccga
    18061 cccggacgac gtcctatttt cgggcaatcg tgcaacgcag ccagaatatg acgaatacga
    18121 tccattatta aacggtgcgc caattaccga acctgtcgct gtagcagctg ctgctaccac
    18181 ggcgacacaa agctgggctg cgccggttga acctgtgact cagacgccgc ctgttgcctc
    18241 tgttgatgtt ccaccttcgc aacctacagt agcctggcag cctgtaccgg gtccacaaac
    18301 gggagagccg gttattgctc ctgcaccgga aggttaccca cagcagtcac aatatgcgca
    18361 gcctgcagtg caatataatg agccgctgca acaaccagta cagccgcagc agccgtatta
    18421 tgcacctgca gctgaacaac ctgcgcaaca gccgtattat gcccctgcgc cagaacaacc
    18481 ggtggcaggt aacgcctggc aagccgaaga gcagcaatcc acttttgctc cacagtctac
    18541 ataccagact gagcaaactt atcagcagcc agccgctcag gagccgttgt accaacagcc
    18601 gcaacccgtt gaacagcagc ctgttgtgga gcctgaaccc gttgtagaag agacaaaacc
    18661 cgcgcgtccg ccgctttact attttgaaga agtggaagag aagcgagccc gtgaacgtga
    18721 acaacttgcg gcctggtatc aaccgattcc agaaccggtt aaagaaccag aaccgatcaa
    18781 atcttcgctg aaagcacctt ctgttgcagc agtacctcca gtagaagccg ctgccgctgt
    18841 ttctccgctg gcatctggcg tgaaaaaagc gacactggcg acgggggctg ccgcaaccgt
    18901 tgccgcgcca gtcttcagtc tggcaaatgg cggtggaccg cgtccgcagg tcaaagaggg
    18961 gattggcccg cagttgccac gaccgaaacg tatccgcgtg ccaactcgtc gtgaattggc
    19021 gtcttacggt attaagctgc cctcacagcg tgcggcggaa gaaaaagccc gtgaagccca
    19081 gcgcaatcag tatgattctg gcgatcagta taacgatgat gaaatcgatg cgatgcagca
    19141 ggatgaactg gcacgtcagt tcgcccagac acagcagcaa cgctatggcg aacagtatca
    19201 acatgatgtg cccgtaaacg cagaagatgc agatgctgcg gcagaggctg aactggctcg
    19261 tcagtttgtg caaactcaac aacaacgtta ttccggcgaa caaccggctg gggcgaatcc
    19321 gttctcgctg gatgattttg aattttcgcc aatgaaagcg ttgctggatg atggtccaca
    19381 cgaaccgctg tttacgccaa ttgttgaacc tgtacagcag ccgcaacaac cggttgcacc
    19441 acagcagcag tatcagcagc cacaacagcc ggttgcgcca cagccgcagt atcagcagcc
    19501 acaacagcag gttgcgccgc agccacaata tcagcagccg caacaaccgg ttgcgccaca
    19561 gcagcaatat cagcagccgc aacaaccagt tgcgccgcag ccgcagtatc aacagccgca
    19621 acaaccggtt gcaccgcagc cgcagtatca gcagccacaa cagccagttg cgccacagcc
    19681 gcaggatacc ctgcttcatc cgctgttgat gcgtaatggc gacagccgtc cgttgcacaa
    19741 accgacgacg ccgctgcctt ctctggattt gctgacaccg ccgccgagcg aagtggagcc
    19801 ggtagatacc tttgcgcttg aacaaatggc gcgtctggtg gaagcgcgtc tggctgattt
    19861 ccgtattaaa gccgatgtcg ttaattactc tccggggcca gttatcactc gctttgaact
    19921 taacctggct cctggcgtaa aagcggcgcg catttctaac ttgtcacggg accttgcccg
    19981 ttcactttcg acggtggcgg tgcgtgtcgt tgaagttatt cctggcaaac cctatgtagg
    20041 tctggagtta ccaaataaaa aacgacaaac cgtttatctg cgcgaagttt tggataacgc
    20101 caaattccgc gataatccgt cgccattaac cgtggtgctg ggtaaagata tcgccggtga
    20161 gccggtggtt gccgatctgg cgaaaatgcc gcacttgttg gttgcgggga ctaccggttc
    20221 cggtaaatct gtcggtgtga acgcgatgat cctgagcatg ctttataaag cacagccaga
    20281 agatgtgcgt ttcatcatga tcgacccgaa aatgctggag ctttcggttt atgaaggcat
    20341 tccgcacctg ttaacggaag tcgttactga tatgaaagat gccgccaacg cgctgcgctg
    20401 gtgtgttaac gagatggagc gtcggtataa actgatgtct gcgctgggtg tgcgtaatct
    20461 ggcgggttat aacgaaaaaa ttgctgaagc cgatcgcatg atgcgtccga ttccagaccc
    20521 gtactggaag ccgggtgaca gtatggatgc ccagcatccg gtgctgaaaa aagaaccata
    20581 cattgtcgtg ttggttgacg aatttgccga cctgatgatg acggtaggta aaaaagtgga
    20641 agagctgata gctcgtctgg cgcaaaaagc ccgtgccgcg ggtatccacc tcgtactggc
    20701 aactcagcgt ccatcggttg atgttattac tggtctgatt aaagcgaata ttccgacccg
    20761 tatcgccttt accgtatcca gtaagattga ctcacgtacc attcttgatc aggctggcgc
    20821 ggaatcactg ctgggtatgg gggatatgct ctactctggg ccgaactcca cgttgccggt
    20881 acgtgtccat ggtgcttttg ttcgcgatca ggaagttcat gccgtggtgc aggactggaa
    20941 agcgcgtggt cgcccacagt atgttgatgg catcacctcc gacagcgaaa gcgaaggtgg
    21001 tgcgggtggt ttcgatggcg ctgaagaact ggatccgttg ttcgatcagg cggtgcagtt
    21061 tgtcactgaa aaacgcaaag cgtcaatttc tggcgtacag cgtcagttcc gcattggtta
    21121 taaccgtgca gcgcgtatta tcgaacagat ggaagcgcag gggattgtca gcgaacaggg
    21181 gcacaacggt aatcgtgaag tgctggcccc accgccgttt gactaattaa tgcattgccg
    21241 gataaggcgc ggtagcgtcg catccggcac tctatcaact gaaaattcag tattttcttc
    21301 tttcctcaag ctgattatta gcctggaata gagagtagag ggaactcccg atcgggagtg
    21361 acgtaatttg aggaataatg atgaaaaaaa ttgctatcac ctgtgcatta ctctcaagct
    21421 tagtagcaag cagcgtttgg gctgatgccg caagcgatct gaaaagccgc ctggataaag
    21481 tcagcagctt ccacgccagc ttcacacaaa aagtgactga cggtagcggc gcggcggtgc
    21541 aggaaggtca gggcgatctg tgggtgaaac gtccaaactt attcaactgg catatgacac
    21601 aacctgatga aagcattctg gtttctgacg gtaaaacact gtggttctat aacccgttcg
    21661 ttgagcaagc tacggcaacc tggctgaaag atgccaccgg taatacgccg tttatgctga
    21721 ttgcccgcaa ccagtccagc gactggcagc agtacaatat caaacagaat ggcgatgact
    21781 ttgtcctgac gccgaaagcc agcaatggca atctgaagca gttcaccatt aacgtgggac
    21841 gtgatggcac aatccatcag tttagcgcgg tggagcagga cgatcagcgc agcagttatc
    21901 agctgaaatc ccagcaaaat ggggctgtgg atgcagcgaa atttaccttc accccgccgc
    21961 aaggcgtcac ggtagatgat caacgtaagt agaggcacct gagtgagcaa tctgtcgctc
    22021 gatttttcgg ataatacttt tcaacctctg gccgcgcgta tgcggccaga aaatttagca
    22081 cagtatatcg gtcagcaaca tttgctggct gcggggaagc cgttgccgcg cgctatcgaa
    22141 gccgggcatt tgcattctat gatcctctgg gggccaccgg gtaccggcaa aacaaccctc
    22201 gctgaagtga ttgcccgcta tgcgaacgct gatgtggaac gtatttctgc cgtcacctct
    22261 ggcgtgaaag agattcgcga ggcgatcgag cgcgcccggc aaaaccgcaa tgcaggtcgc
    22321 cgcactattc tttttgttga cgaagttcac cgtttcaaca aaagccagca ggatgcattt
    22381 ctgccacata ttgaagacgg caccatcact tttattggcg caaccactga aaacccgtcg
    22441 tttgagctta attcggcact gctttcccgt gcccgtgttt atctgttgaa atccctgagt
    22501 acagaggata ttgagcaagt actaactcag gcgatggaag acaaaacccg tggctatggt
    22561 ggtcaggata ttgttctgcc agatgataca cgacgtgcca ttgcagaact ggtgaatggc
    22621 gacgcgcgcc gggcgttaaa tacgctggaa atgatggcgg atatggccga agtctatgat
    22681 agcggtaagc gggtcctgaa gcctgaatta ctgatcgaaa tcgccggtga acgtagcgcc
    22741 cgctttgata acaaaggcga tcgcttttac gatctaattt ccgcactgca taagtcggta
    22801 cgtggtagcg cacccgatgc ggcgctgtac tggtatgcgc gaattattac cgctggtggc
    22861 gatccgttat atgtcgcgcg tcgctgtctg gcgattgcgt ctgaagacgt cggtaatgcc
    22921 gatccacggg cgatgcaggt ggcaattgcg gcctgggatt gctttactcg cgttggcccg
    22981 gcggaaggtg aacgcgccat tgctcaggcg attgtttacc tggcctgcgc gccaaaaagc
    23041 aacgctgtct acactgcgtt taaagccgcg ctggccgatg ctcgcgaacg cccggattat
    23101 gacgtgccgg ttcatttgcg taatgcgccg acgaaattaa tgaaggaaat gggctacggg
    23161 caggaatatc gttacgctca tgatgaagca aacgcttatg ctgccggtga ggtttacttc
    23221 ccgccggaaa tagcacaaac acgctattat ttcccgacaa acaggggcct tgaaggcaag
    23281 attggtgaaa agctcgcctg gctggctgaa caggatcaaa atagccccat aaaacgctac
    23341 cgttaatgtt atcgttgcgg taatgttgtt actgtatccc tgtggtcgca ggctgtggcc
    23401 acatctccca tttaattcga taagcacagg ataagcatgc tcgatcccaa tctgctgcgt
    23461 aatgagccag acgcagtcgc tgaaaaactg gcacgccggg gctttaagct ggatgtagat
    23521 aagctgggcg ctcttgaaga gcgtcgtaaa gtattgcagg tcaaaacgga aaacctgcaa
    23581 gcggagcgta actcccgatc gaaatccatt ggccaggcga aagcgcgcgg ggaagatatc
    23641 gagcctttac gtctggaagt gaacaaactg ggcgaagagc tggatgcagc aaaagccgag
    23701 ctggatgctt tacaggctga aattcgcgat atcgcgctga ccatccctaa cctgcctgca
    23761 gatgaagtgc cggtaggtaa agacgaaaat gacaacgttg aagtcagccg ctggggtacc
    23821 ccgcgtgagt ttgactttga agttcgtgac cacgtgacgc tgggtgaaat gcactctggc
    23881 ctcgactttg cagctgcagt taagctgact ggttcccgct ttgtggtaat gaaagggcag
    23941 attgctcgca tgcaccgcgc actgtcgcag tttatgctgg atctgcatac cgaacagcat
    24001 ggctacagtg agaactatgt tccgtacctg gttaaccagg acacgctgta cggtacgggt
    24061 cagctgccga aatttgctgg cgatctgttc catactcgtc cgctggaaga agaagcagac
    24121 accagtaact atgcgctgat cccaacggca gaagttccgc tgactaacct ggtgcgcggt
    24181 gaaatcatcg atgaagatga tctgccaatt aagatgatcg cccacacccc atgcttccgt
    24241 tctgaagccg gttcatatgg tcgtgacacc cgtggtctga tccgtatgca ccagttcgac
    24301 aaagttgaaa tggtgcagat cgtgcgccca gaagactcaa tggcggcgct ggaagagatg
    24361 actggtcatg cagaaaaagt cctgcagttg ctgggcctgc cgtaccgtaa aatcatcctt
    24421 tgcactggcg acatgggctt tggcgcttgc aaaacttacg acctggaagt atggatcccg
    24481 gcacagaaca cctaccgtga gatctcttcc tgctccaacg tttgggattt ccaggcacgt
    24541 cgtatgcagg cacgttgccg cagcaagtcg gacaagaaaa cccgtctggt tcataccctg
    24601 aacggttctg gtctggctgt tggtcgtacg ctggttgcag taatggaaaa ctatcagcag
    24661 gctgatggtc gtattgaagt accagaagtt ctgcgtccgt atatgaacgg actggaatat
    24721 attggctaat acccaatttt tctgaatcta aaaagcgcct ttagggcgct ttttttgtct
    24781 ccctttgata ccgaacaata attactcctc acttacacgt aatactactt tcgagtgaaa
    24841 atctaccgat ctctttgatt ttcaaattat tctatgtata caagcctata tagcgagctg
    24901 ctatagaaat aattacacaa tacggtttgt tactggaatc aatcgtgatc aagcttgagt
    24961 gagccattat gaaaacgaaa atccctgatg cggtattggc tgctgaggag agtcgccgtg
    25021 gtttggtaaa aacgacagcg atcggcggcc tggcaatggc cagcagcgca ttaacattac
    25081 cttttagtcg gattgcgcac gctgtcgata gcgccattcc aacaaaatca gacgaaaagg
    25141 ttatctggag cgcctgtaca gttaactgtg gtagtcgctg cccgctacgt atgcacgtcg
    25201 tggacggtga aatcaaatat gtcgaaacgg acaataccgg cgatgacaat tacgacggcc
    25261 tacactaggt tcgcgcctgc ctgcgtgggc gttccatgcg tcaccgtgtc tacaatccgg
    25321 accgcctgaa atatccgatg aaacgagtcg gggcgcgcgg tgaaggcaaa ttcgagcgca
    25381 ttagctggga agaagcctac gacatcatcg cgaccaatat gcagcgcctg atcaaagagt
    25441 acggcaacga gtctatctat ctgaactatg gcaccggtac gctgggcggc accatgaccc
    25501 gctcctggcc gccgggaaat accctggtcg cgcgcctgat gaactgctgc ggcggttatc
    25561 ttaaccatta cggtgactac tcttccgcgc aaattgcgga aggtttgaac catacctacg
    25621 gcggctgggc agatggtaac agcccgtcgg atatcgaaaa cagtaaattg gtagtgctgt
    25681 ttggtaataa cccaggcgaa acgcgaatga gtggcggtgg ggtgacttac tatcttgaac
    25741 aggcacgcca gaaatctaat gctcgcatga tcatcatcga tccgcgctat accgacaccg
    25801 gtgccgggcg cgaagatgag tggatcccta ttcgtccagg aacagatgcc gcactggtta
    25861 acggtctggc gtacgtcatg atcactaaaa acctggtgga tcaggcattc ctcgataaat
    25921 attgcgttgg ctatgacgag aaaaccctgc ccgccagtgc gccgaaaaat ggtcattata
    25981 aagcttatat tctgggtgaa gggccagatg gcgtggctaa aacaccggaa tgggcctcgc
    26041 aaatcaccgg tgttccggca gacaaaatca tcaaactggc tcgtgaaatc ggcagtacca
    26101 aaccggcgtt tatcagccag ggatggggcc cgcagcgtca cgctaacggt gaaatcgcaa
    26161 cccgtgctat ctcgatgctg gcgattctga ccggtaacgt tggtattaat ggagggaaca
    26221 gcggcgcgcg tgaaggttca tacagcttac cgtttgtccg tatgccgacc ttggaaaacc
    26281 cgatccagac cagcatttcg atgtttatgt ggaccgatgc cattgaacgt ggcccggaaa
    26341 tgacggcgct gcgtgatggt gtgcgcggga aagataagct ggatgtgccg atcaaaatga
    26401 tctggaacta tgccggtaac tgcctgatta accagcattc tgaaatcaac cgtacccatg
    26461 aaatcctcca ggatgataag aagtgcgagc tgattgtggt tatcgactgc cacatgacct
    26521 catcggcgaa atatgctgac atcctgctgc ctgactgcac cgcttccgaa cagatggact
    26581 ttgcactgga tgcatcctgc gggaatatgt cttacgtgat tttcaacgat caggtgatta
    26641 aaccgcgctt tgaatgtaag accatctatg aaatgaccag cgaactggca aaacgtcttg
    26701 gcgttgagca acagtttact gaaggccgta cctaggaaga gtggatgcgg catctgtatg
    26761 cccagtcgcg ggaagcgatt cctgaactgc caacgtttga agagttccgc aagcagggga
    26821 tctttaaaaa gcgcgaccca caagggcatc acgttgctta taaagccttc cgtgaagatc
    26881 cgcaggcaaa cccactgact acgccatcgg gcaaaattga gatttattcg caggcgctgg
    26941 ctgacattgc cgctacctgg gaattgccag aaggcgatgt gatcgatcca ctgccgatct
    27001 acacgccggg ctttgaaagt tatcaggatc cgctgaacaa acagtatccg ctgcagctta
    27061 caggtttcca ctataaatct cgcgttcact caacttacag caacgttgat gtgctgaaag
    27121 cggcttgccg tcaggaaatg tggatcaacc cgcttgatgc ccaaaaacgc ggtatccaca
    27181 acggcgataa agtcaggatc tttaacgatc gtggtgaggt tcatattgag gcgaaagtga
    27241 cgccacgaat gatgccgggc gtggtcgcac tgggtgaagg cgcctggtat gacccggatg
    27301 caaaacgtgt cgataagggc ggttgtatca acgtactgac cactcaacgt ccgtctcctc
    27361 tcgctaaggg gaatccgtca catacaaacc ttgttcaggt tgaaaaggtg taaggagtaa
    27421 ccgatgacaa cccagtatgg attttttatt gattccagcc gttgcaccgg ttgcaaaacc
    27481 tgcgagctgg cttgtaaaga ctacaaagat ttgacgccag aagtcagttt ccgccgcatt
    27541 tatgaatatg ctggcggcga ctggcaggaa gataacggtg tctggcacca gaacgtgttt
    27601 gcctactatc tgtcgatttc gtgcaaccac tgcgaagatc cggcttgtac taaagtctgc
    27661 ccgagcggtg cgatgcataa acgcgaagat ggttttgttg tggtcgatga agatgtatgc
    27721 attggctgcc gttactgtca tatggcttgc ccgtatggcg caccgcaata taacgaaacg
    27781 aaaggccata tgaccaaatg cgatggttgt tatgaccgtg ttgcggaggg taaaaagccg
    27841 atctgtgttg aatcttgccc gctgcgggcg ctggatttcg gccctatcga cgagctgcgt
    27901 aaaaaacatg gcgatctggc ggccgttgcg ccgttgccgc gagctcactt taccaaaccg
    27961 aatattgtga tcaaacccaa tgccaatagc cgcccgaccg gggataccac tggctatctg
    28021 gcaaacccga aggaggtgtg agatgggaag tggatggcat gaatggccgc tgatgatctt
    28081 cacggtcttc gggcaatgtg tggcgggtgg ttttatcgtt ctggctttgg cgctgctcaa
    28141 aggcgacctg cgagcagaag cccagcagcg tgttatcgcc tgcatgtttg gcttatgggt
    28201 actgatgggc attggcttta tcgcctctat gctccatctt ggttcaccga tgcgcgcctt
    28261 taactcgctc aaccgggtag gggcttcagc actcagtaac gaaatcgcca gcggttcgat
    28321 cttttttgct gtaggcggca tcggctggtt gctggcaatg ctgaaaaagc tgtcaccggc
    28381 attgcgtacg ctatggctgg tggtgacgat ggttcttggc gtcgtctttg tctggatgat
    28441 ggtgcgtgtg tataacagca ttgataccgt tccgacctgg tacagcatct ggacgccgat
    28501 gggctgggcg atgcgtctgc tgccagccat ttctgtactg gcactggtgg tgagtggcgt
    28561 gatgtcagtg atgcagggca cagagctggc aactattcat agttctgtgc agcaggccgc
    28621 agcgctggtg ccggactatg gtgcgctgat gtcctggcgg atcgtgcttt tggccgttgc
    28681 cctgtgcttg tggattgcac cacagctaaa aggttatcag cctgcggttc cgctactatc
    28741 cgtctcattc attctgcttc tggcagggga attaatcggt cgcggcgtat tctatggttt
    28801 gcatatgacc gtggggatgg ccgtcgcaag ctaatcagta acaaccgggg tttcggcccc
    28861 ggttctcttt tatttctgct tcgttaacgt gtcataactg gtcatcaaat tacgataatc
    28921 aggaatatgg ttagagaaca acgtcgccag tccttcaata tcattacgcc agtcacggtg
    28981 cagctcgcaa gccacgccga accatgtcat caattgcgca ccagcttgcg acatgcgatc
    29041 ccacgccgaa tgccgggtaa tttcattaaa ggtccctgat gcatcagtca cgacaaagac
    29101 atcaaaccct tcttcaatgg ctgaaagcgc cgggaatgcc acgcaaactt cggttaccac
    29161 accggcaata attaactgtt ttttacctgt cgctttgact gcttttacaa aatcttcgtt
    29221 atcccaggcg ttaatattgc ccgggcgagc aatgtaaggt gcatcgggga attgtgcttt
    29281 cagttctaga actaatgggc cgttaggacc ggtttcaaaa ctggtggtaa gaatagttgg
    29341 taagttgaaa tacttagcca ggtcacccag cgccagcacg ttatttttaa acttatcggg
    29401 ttcgatatcc cgtacaaggg aaagtaaacc agcctggtga tcaacaagca aaacggcagc
    29461 atcattttta tcaagacgaa catacggttt ggtcatcctc ttctcctttc gagagtcagt
    29521 ggtacgtctg aaatcggtca gacgcgatgc attgctctga aagcatagac gggaaatatg
    29581 agtttgctgt gaccatgaaa tttttcgact gaacgaagcg tatgaaattt gtgttagatc
    29641 aataaaaaca atcagatgag attaatcatc tcttcagcat tagcgttatg aatgagtaat
    29701 ataaatcgat aataccgccg ctgcgatatg cgcgtatttt cacccattga caatgttttt
    29761 ggcggtggca tgatgcgcat gaaatttgaa cttcctcacg gttttaattc atgtccacgt
    29821 atacccggcc tgtcatgctt ttgctgtctg gcctgctttt gttgactctg gcgatcgcgg
    29881 tgttaaatac actcgtgccg ctttggctcg cccaggaaca catgtccaca tggcaggtag
    29941 gcgttgtcag ctcatcctat tttaccggca accttgtcgg tacattgctg acagggtatg
    30001 tcattaaacg cattggcttt aaccgcagct attatctgtc ctccttcatt tttgctgctg
    30061 gctgtgccgg ccttggcctg atgattggtt tctggagctg gttggcgtgg cgttttgtcg
    30121 cgggcgtcgg ctgtgccatg atttgggtgg ttgttgagag cgcgctgatg tgcagtggga
    30181 cgtcacgtaa ccgtgggcgt ttgcttgctg cgtatatgat ggtttattac gtgggaacgt
    30241 ttttaggcca gttactggtc agcaaagtct caaccgagct gatgagcgta ttgccgtggg
    30301 ttacaggttt gacattggca gggatcttac cgctgttgtt tacgcgtgtc ctgaatcagc
    30361 aggctgaaaa ccatgattcg acgtcaatta cggcaatgct aaaactccgt caggcgcggc
    30421 ttggcgtgaa tggctgcatt atctcaggaa tcgttctggg atctctatat ggcctgatgc
    30481 ctctgtacct caatcacaaa ggggtgagca atgccagtat tggtttctgg atggcggtac
    30541 tggtcagtgc gggtatcctc ggacaatggc cgattggacg tctggcggat aagtttggtc
    30601 gactgctggt tttgcgtgtt caggtctttg tcgtcattct cggcagtatt gcgatgctta
    30661 gccaggcggc gatggcccca gcgttattca tcctcggtgc cgctggcttt acgctatatc
    30721 cggtggcgat ggcctgggct tgtgagaaag ttgaacatca tcaactggtg gcgatgaacc
    30781 aagccttact gttgagctat accgtgggaa gtctgcttgg cccgtcattt agcgctatgc
    30841 taatgcagaa tttctccgat aatttattgt ttatcatgat cgccagcgta tcgtttatct
    30901 atttgctgat gctgcttcgc aacgccggtc atacgccgaa acccgttgct cacgtgtaaa
    30961 tgaattcaag caaagtgtga acttactgtt tcacactctg cttttttgtt tcttctatct
    31021 gacttgcttt tttccaaatt ttattcg
//
LOCUS       DAJTQY010000044        30466 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.044, whole genome shotgun sequence.
ACCESSION   DAJTQY010000044 DAJTQY010000000
VERSION     DAJTQY010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30466
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.044"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            <1..470
                     /gene="tuf"
                     /locus_tag="OO349_002051"
     CDS             <1..470
                     /gene="tuf"
                     /locus_tag="OO349_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR5867119.1"
                     /translation="GEEVEIVGIKETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIK
                     REEIERGQVLAKPGTIKPHTKFESEVYILSKDEGGRHTPFFKGYRPQFYFRTTDVTGT
                     IELPEGVEMVMPGDNIKMVVTLIHPIAMDDGLRFAIREGGRTVGAGVVAKVLS"
     gene            700..1083
                     /gene="secE"
                     /locus_tag="OO349_002052"
     CDS             700..1083
                     /gene="secE"
                     /locus_tag="OO349_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HCR5867120.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLAAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATIAFAREARTEVRKVIWPIRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            1085..1630
                     /gene="nusG"
                     /locus_tag="OO349_002053"
     CDS             1085..1630
                     /gene="nusG"
                     /locus_tag="OO349_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HCR5867121.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1789..2217
                     /gene="rplK"
                     /locus_tag="OO349_002054"
     CDS             1789..2217
                     /gene="rplK"
                     /locus_tag="OO349_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HCR5867122.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            2221..2925
                     /gene="rplA"
                     /locus_tag="OO349_002055"
     CDS             2221..2925
                     /gene="rplA"
                     /locus_tag="OO349_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HCR5867123.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3338..3835
                     /gene="rplJ"
                     /locus_tag="OO349_002056"
     CDS             3338..3835
                     /gene="rplJ"
                     /locus_tag="OO349_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HCR5867124.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3902..4267
                     /gene="rplL"
                     /locus_tag="OO349_002057"
     CDS             3902..4267
                     /gene="rplL"
                     /locus_tag="OO349_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HCR5867125.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4587..8615
                     /gene="rpoB"
                     /locus_tag="OO349_002058"
     CDS             4587..8615
                     /gene="rpoB"
                     /locus_tag="OO349_002058"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HCR5867126.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKINQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8692..12915
                     /gene="rpoC"
                     /locus_tag="OO349_002059"
     CDS             8692..12915
                     /gene="rpoC"
                     /locus_tag="OO349_002059"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HCR5867127.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(13157..14290)
                     /gene="thiH"
                     /locus_tag="OO349_002060"
     CDS             complement(13157..14290)
                     /gene="thiH"
                     /locus_tag="OO349_002060"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HCR5867128.1"
                     /translation="MKTFSNRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQQAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYTRHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAVLTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(14287..15057)
                     /gene="thiG"
                     /locus_tag="OO349_002061"
     CDS             complement(14287..15057)
                     /gene="thiG"
                     /locus_tag="OO349_002061"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HCR5867129.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVALLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHATQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEVGLLARQSGPGSRSYFAHATSPLTGFLEASA"
     gene            complement(15059..15259)
                     /gene="thiS"
                     /locus_tag="OO349_002062"
     CDS             complement(15059..15259)
                     /gene="thiS"
                     /locus_tag="OO349_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HCR5867130.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(15243..15998)
                     /gene="thiF"
                     /locus_tag="OO349_002063"
     CDS             complement(15243..15998)
                     /gene="thiF"
                     /locus_tag="OO349_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HCR5867131.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDINSPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVAQADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQQPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(15991..16626)
                     /gene="thiE"
                     /locus_tag="OO349_002064"
     CDS             complement(15991..16626)
                     /gene="thiE"
                     /locus_tag="OO349_002064"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HCR5867132.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16626..18521)
                     /gene="thiC"
                     /locus_tag="OO349_002065"
     CDS             complement(16626..18521)
                     /gene="thiC"
                     /locus_tag="OO349_002065"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HCR5867133.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARADTEELTVRSSDYTRTRLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     DMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLHKEEA"
     regulatory      complement(18602..18702)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18755..19231)
                     /gene="rsd"
                     /locus_tag="OO349_002066"
     CDS             complement(18755..19231)
                     /gene="rsd"
                     /locus_tag="OO349_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HCR5867134.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            19326..20099
                     /gene="nudC"
                     /locus_tag="OO349_002067"
     CDS             19326..20099
                     /gene="nudC"
                     /locus_tag="OO349_002067"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HCR5867135.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLIQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSLLLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            20139..21203
                     /gene="hemE"
                     /locus_tag="OO349_002068"
     CDS             20139..21203
                     /gene="hemE"
                     /locus_tag="OO349_002068"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HCR5867136.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRHELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            21360..22031
                     /gene="nfi"
                     /locus_tag="OO349_002069"
     CDS             21360..22031
                     /gene="nfi"
                     /locus_tag="OO349_002069"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HCR5867137.1"
                     /translation="MDLASLRAQQIELASSVICEDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSKPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            22074..22664
                     /locus_tag="OO349_002070"
     CDS             22074..22664
                     /locus_tag="OO349_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HCR5867138.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMGDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLRESGESNIGIIFQQ"
     gene            22851..23123
                     /gene="hupA"
                     /locus_tag="OO349_002071"
     CDS             22851..23123
                     /gene="hupA"
                     /locus_tag="OO349_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HCR5867139.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            23136..23831
                     /locus_tag="OO349_002072"
     CDS             23136..23831
                     /locus_tag="OO349_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HCR5867140.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23833..24258)
                     /gene="zraP"
                     /locus_tag="OO349_002073"
     CDS             complement(23833..24258)
                     /gene="zraP"
                     /locus_tag="OO349_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HCR5867141.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24496..25872
                     /gene="zraS"
                     /locus_tag="OO349_002074"
     CDS             24496..25872
                     /gene="zraS"
                     /locus_tag="OO349_002074"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HCR5867142.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMYR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            25869..27194
                     /gene="zraR"
                     /locus_tag="OO349_002075"
     CDS             25869..27194
                     /gene="zraR"
                     /locus_tag="OO349_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HCR5867143.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGAQD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(27191..28480)
                     /gene="purD"
                     /locus_tag="OO349_002076"
     CDS             complement(27191..28480)
                     /gene="purD"
                     /locus_tag="OO349_002076"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HCR5867144.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALVYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDNGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLSV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28492..30081)
                     /gene="purH"
                     /locus_tag="OO349_002077"
     CDS             complement(28492..30081)
                     /gene="purH"
                     /locus_tag="OO349_002077"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HCR5867145.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDVAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7142 a         7929 c         8386 g         7009 t
ORIGIN      
        1 ttggtgaaga agttgaaatc gttggtatca aagagactca gaagtctacc tgtactggcg
       61 ttgaaatgtt ccgcaaactg ctggacgaag ggcgtgctgg tgagaacgta ggtgttctgc
      121 tgcgtggtat caaacgtgaa gaaatcgaac gtggtcaggt actggctaag ccgggcacca
      181 tcaagccgca caccaagttc gaatctgaag tgtacattct gtccaaagat gaaggcggtc
      241 gtcatactcc gttcttcaaa ggctaccgtc cgcagttcta cttccgtact actgacgtga
      301 ctggtaccat cgaactgccg gaaggcgtag agatggtaat gccgggcgac aacatcaaaa
      361 tggttgttac cctgatccac ccgatcgcga tggacgacgg tctgcgtttc gcaatccgtg
      421 aaggcggccg taccgttggc gcgggcgttg tagcaaaagt tctgagctaa ttgccgataa
      481 catttgacgc aatgcgcact aaaagggcat catttgatgc cctttttgca cgctttcgta
      541 ccagaacctg gctcatcagt gattttcttt gtcataatca ttgctgagac aggctctgtt
      601 gagggcgtat aatccgaaaa gctaatacgc gtttcgattt ggtttgcctc gcgatcgcgg
      661 ggtgaaaatg tttgtagaaa acttctgaca ggttggttta tgagtgcgaa taccgaagct
      721 caaggaagcg ggcgcggcct ggaagcgatg aagtgggtcg ttgtggtggc attgctcctg
      781 gcggcgattg tcggcaacta cctttatcgc gacattatgc tgccgctgcg tgcgctggcc
      841 gtagtaattc tgattgctgc agcgggtggt gtcgcgctgt taacgacaaa aggtaaagct
      901 accattgctt ttgcccgtga agcgcgtacc gaagtccgta aggtcatttg gccgattcgc
      961 caggaaacat tgcacaccac gctgattgtg gctgcggtta ccgcagtaat gtcactgatc
     1021 ctgtggggac tggatggtat tctggttcgc ctggtatcct ttatcactgg cctgaggttc
     1081 tgagatgtct gaagctccta aaaagcgctg gtacgtcgtt caggcgtttt ccggttttga
     1141 aggccgcgta gcaacgtcgc tgcgtgagca tatcaaatta cacaacatgg aagatttgtt
     1201 tggtgaagtc atggtaccaa ccgaagaagt ggttgaaatc cgtggcggtc agcgtcgcaa
     1261 aagcgaacgt aaattcttcc ctggctacgt cctcgttcag atggtgatga acgacgcgag
     1321 ctggcacttg gtgcgcagcg taccgcgtgt gatgggcttc atcggcggta cttccgatcg
     1381 tcctgcgcca atcagcgata aagaagtcga tgcgattatg aaccgcctgc agcaggttgg
     1441 tgataagccg cgtccgaaaa cgctgtttga accgggtgaa atggtccgtg ttaatgatgg
     1501 tccgttcgct gacttcaacg gtgttgttga agaagtggat tacgagaaat ctcgtctgaa
     1561 agtgtctgtt tctatcttcg gtcgtgcgac cccggtagag ctggacttca gccaggttga
     1621 aaaagcctaa cccagcgatc aaaaaagcgg cgatttaatc gttgcacaag gcgtgagatt
     1681 ggaatacaat ttcgcgcctt ttgtttttat gggccttgcc cgtaaaacga ttttttatat
     1741 cacggggagc ctctcagagg cgttattacc caacttgagg aatttataat ggctaagaaa
     1801 gtacaagcct atgtcaagct gcaggttgca gctggtatgg ctaacccgag tccgccagta
     1861 ggtccggctc tgggtcagca gggcgtaaac atcatggaat tctgcaaagc gttcaacgca
     1921 aaaactgatt ccatcgaaaa aggtctgccg attccggtag taatcaccgt ttacgctgac
     1981 cgttctttca ctttcgttac caagaccccg ccggcagcag ttctgctgaa aaaagcggca
     2041 ggtatcaagt ctggttccgg taagccgaac aaagacaaag tgggtaaaat ttcccgcgct
     2101 cagctgcagg aaatcgcgca gaccaaagct gccgacatga ctggtgccga cattgaagcg
     2161 atgactcgct ccatcgaagg tactgcacgt tccatgggcc tggtagtgga ggactaagaa
     2221 atggctaaac tgaccaagcg catgcgtgtt atccgtgaga aagttgatgc aaccaaacag
     2281 tacgacatca acgaagctat cgcactgctg aaagagctgg cgactgctaa attcgtagaa
     2341 agcgtggacg tagctgttaa cctcggcatc gacgctcgta aatctgacca gaacgtacgt
     2401 ggtgcaactg tactgccgca cggtactggc cgttccgttc gcgtagccgt atttacccaa
     2461 ggtgcaaacg ctgaagctgc taaagctgca ggcgcagaac tggtaggtat ggaagatctg
     2521 gctgaccaga tcaagaaagg cgaaatgaac tttgacgttg ttattgcttc tccggatgca
     2581 atgcgcgttg ttggccagct gggccaggtt ctgggtccgc gtggcctgat gccaaacccg
     2641 aaagtgggta ctgtaacacc gaacgttgct gaagcggtta aaaacgctaa agctggccag
     2701 gttcgttacc gtaacgacaa aaacggcatc atccacacca ccatcggtaa agtggacttt
     2761 gacgctgaca aactgaaaga aaacctggaa gctctgctgg ttgcgctgaa aaaagcaaaa
     2821 ccgactcagg cgaaaggcgt gtacatcaag aaagttagca tctccaccac catgggtgca
     2881 ggtgttgcag ttgaccaggc tggcctgagc gcttctgtaa actaatgcct ttacgtgggc
     2941 ggtgattttg tctacaatct tacccccacg tataatgctt aatgcagacg tatatccgag
     3001 atattcgggt tgtggcaagg cggcaactga gtgagtcgcc aagagcatag ctaactatgt
     3061 gactggtgcg aatgaaggaa gccaacgccg ccacaagttg aatagcgacg gatagaaaag
     3121 atttgttcgt tggagcctgg cctatccagg cctccgtcga agaccgcagg agtttcgcaa
     3181 gaaacttaat cccctgcgta gacggtgaca gaacgctaag attattcttt tatattctgg
     3241 cttgtttctg ctcaccgtaa ttaagacgct ctctccgttt ggaggagtga agtgagttcc
     3301 agagattttc tctggcaaac atccaggagc aaagctaatg gctttaaatc ttcaagacaa
     3361 acaagcgatt gttgctgaag tcagcgaagt agccaaaggc gcgctgtctg cagtagttgc
     3421 ggattcccgt ggcgtaactg tagataaaat gactgaactg cgtaaagcag gtcgcgaagc
     3481 tggcgtatac atgcgtgttg ttcgtaacac cctgctgcgc cgtgctgttg aaggtactcc
     3541 gttcgagtgc ctgaaagacg cgtttgttgg tccgaccctg attgcatact ctatggaaca
     3601 cccgggcgct gctgctcgtc tgttcaaaga gttcgcgaaa gcgaatgcaa aatttgaggt
     3661 caaagccgct gcctttgaag gtgagctgat cccggcgtct cagatcgacc gcctggcaac
     3721 tctgccgacc tacgaagaag caattgcacg cctgatggca accatgaaag aagcttcggc
     3781 tggcaaactg gttcgtactc tggctgctgt acgcgatgcg aaagaagctg cttaatcgca
     3841 gttatctttt taacgcattc gcttacgtat aaacttattc tgatattcag gaacaattta
     3901 aatgtctatc actaaagatc aaatcattga agcagttgca gctatgtctg taatggacgt
     3961 tgtagaactg atctctgcaa tggaagaaaa attcggtgtt tccgctgctg ctgctgtagc
     4021 tgtagctgct ggcccggttg aagctgctga agaaaaaact gaattcgacg taattctgaa
     4081 agctgctggc gctaacaaag ttgctgttat caaagcagta cgtggcgcaa ctggcctggg
     4141 tctgaaagaa gctaaagact tggtagaatc tgcaccggct gctctgaaag aaggcgtgag
     4201 caaagacgac gcagaagcac tgaaaaaagc tctggaagaa gctggcgctg aagttgaagt
     4261 taaataagcc aacccttccg gttgcagcct gagaaatcag gctgatggct ggtgactttt
     4321 tagtcaccag cctttttgcg ctgtaaggcg ccagtagcgt ttcacactgt ttgactactg
     4381 ctgtgccttt caatgcttgt ttctatcgac gacttaatat actgcgacag gacgtccgtt
     4441 ctgtgtaaat cgcaatgaaa tggtttaagc gtgatagcaa caggcattgc ggaaagtgtt
     4501 ccattttccg gtcaacaaaa tagtgttgca caaactgtcc gctcaatgga cagatgggtc
     4561 gacttgtcag cgagctgagg aaccctatgg tttactccta taccgagaaa aaacgtattc
     4621 gtaaggattt tggtaaacgt ccacaagttc tggatgtacc ttatctcctt tctatccagc
     4681 ttgactcgtt tcagaaattt atcgagcaag atcctgaagg gcagtatggt ctggaagctg
     4741 ctttccgttc cgtattcccg attcagagct acagcggtaa ttccgagctg caatacgtca
     4801 gctaccgcct tggcgaaccg gtgtttgacg tccaggaatg tcaaatccgt ggcgtgacct
     4861 attccgcacc gctgcgcgtt aaactgcgtc tggtgatcta tgagcgcgaa gcgccggaag
     4921 gcaccgtaaa agacattaaa gaacaagaag tctacatggg cgaaattccg ctcatgacag
     4981 acaacggtac ctttgttatc aacggtactg agcgtgttat cgtttcccag ctgcaccgta
     5041 gtccgggcgt cttctttgac tccgacaaag gtaaaaccca ctcttcgggt aaagtgctgt
     5101 ataacgcgcg catcatccct taccgtggtt cctggctgga cttcgaattc gatccgaagg
     5161 acaacctgtt cgtacgtatc gaccgtcgcc gtaaactgcc tgcgaccatc attctgcgcg
     5221 ccctgaacta caccacagag cagatcctcg acctgttctt tgaaaaagtt atctttgaaa
     5281 tccgtgataa caagctgcag atggaactgg tgccggaacg cctgcgtggt gaaaccgcat
     5341 cttttgacat cgaagctaac ggtaaagtgt acgtagaaaa aggccgccgt atcactgcgc
     5401 gccacattcg ccagctggaa aaagacgacg tcaaactgat cgaagtcccg gttgagtaca
     5461 tcgcaggtaa agtggttgct aaagactata ttgatgagtc taccggcgag ctgatctgcg
     5521 cagcgaacat ggagctgagc ctggatctgc tggctaagct gagccagtct ggtcacaagc
     5581 gtatcgaaac gctgttcacc aacgatctgg atcacggccc atatatctct gaaaccttac
     5641 gtgtcgaccc aactaacgac cgtctgagcg cactggtaga aatctaccgc atgatgcgcc
     5701 ctggcgagcc gccgactcgt gaagcagctg aaagcctgtt cgagaacctg ttcttctccg
     5761 aagaccgtta tgacttgtct gcggttggtc gtatgaagtt caaccgttct ctgctgcgcg
     5821 aagaaatcga aggttccggt attctgagca aagacgacat cattgatgtt atgaaaaagc
     5881 tcatcgatat ccgtaacggt aaaggcgaag tcgatgatat cgaccacctc ggcaaccgtc
     5941 gtatccgttc cgttggcgaa atggcggaaa accagttccg cgttggcctg gtacgtgtag
     6001 agcgtgcggt gaaagagcgt ctgtctctgg gcgatctgga taccctgatg cctcaggata
     6061 tgatcaacgc caagccgatt tccgcagcag tgaaagagtt cttcggttcc agccagctgt
     6121 ctcagtttat ggaccagaac aacccgctgt ctgagattac gcacaaacgt cgtatctccg
     6181 cactcggccc aggcggtctg acccgtgaac gtgcaggctt cgaagttcga gacgtacacc
     6241 cgactcacta cggtcgcgta tgtccaatcg aaacccctga aggtccgaac atcggtctga
     6301 tcaactctct gtccgtgtac gcacagacta acgaatacgg cttccttgag actccgtatc
     6361 gtaaagtgac cgacggtgtt gtaactgacg aaattcacta cctgtctgct atcgaagaag
     6421 gcaactacgt tatcgcccag gcgaactcca acctggatga agaaggccac ttcgtagaag
     6481 acctggtaac ttgccgtagc aaaggcgaat ccagcttgtt cagccgcgac caggttgact
     6541 acatggacgt atccacccag caggtggtat ccgtcggtgc gtccctgatc ccgttcctgg
     6601 aacacgatga cgccaaccgt gcattgatgg gtgcgaatat gcaacgtcag gccgttccga
     6661 ctctgcgcgc tgataagccg ctggttggta ctggtatgga acgtgctgtt gccgttgact
     6721 ccggtgtaac tgcggtagct aaacgtggtg gtgtcgttca gtacgtggat gcttcccgta
     6781 tcgttatcaa agttaacgaa gacgagatgt atccgggtga agcaggtatc gacatctaca
     6841 acctgaccaa atacacccgt tctaaccaga acacctgtat caaccagatg ccgtgtgtgt
     6901 ctctgggtga accggttgaa cgtggcgacg tgctggcaga cggtccgtcc accgacctcg
     6961 gtgaactggc gcttggtcag aacatgcgcg tagcgttcat gccgtggaat ggttacaact
     7021 tcgaagactc catcctcgta tccgagcgtg ttgttcagga agaccgtttc accaccatcc
     7081 acattcagga actggcgtgt gtgtcccgtg acaccaagct gggtccggaa gagatcaccg
     7141 ctgacatccc gaacgtgggt gaagctgcgc tctccaaact ggatgaatcc ggtatcgttt
     7201 acattggtgc ggaagtgacc ggtggcgaca ttctggttgg taaggtaacg ccgaaaggtg
     7261 aaactcagct gaccccagaa gaaaaactgc tgcgtgcgat cttcggtgag aaagcctctg
     7321 acgttaaaga ctcttctctg cgcgtaccaa acggtgtatc cggtacggtt atcgacgttc
     7381 aggtctttac tcgcgatggc gtagaaaaag acaaacgtgc gctggaaatc gaagaaatgc
     7441 agctcaaaca ggcgaagaaa gacctgtctg aagaactgca gatcctcgaa gcgggtctgt
     7501 tcagccgtat ccgtgctgtg ctggtagccg gtggcgttga agctgagaag ctcgacaaac
     7561 tgccgcgcga tcgctggctg gagctgggcc tgacagacga agagaaacaa aatcagctgg
     7621 aacagctggc tgagcagtat gacgaactga aacacgagtt cgagaagaaa ctcgaagcga
     7681 aacgccgcaa aatcaaccag ggcgacgatc tggcaccggg cgtgctgaag attgttaagg
     7741 tatatctggc ggttaaacgc cgtatccagc ctggtgacaa gatggcaggt cgtcacggta
     7801 acaagggtgt aatttctaag atcaacccga tcgaagatat gccttacgat gaaaacggta
     7861 cgccggtaga catcgtactg aacccgctgg gcgtaccgtc tcgtatgaac atcggtcaga
     7921 tcctcgaaac ccacctgggt atggctgcga aaggtatcgg cgacaagatc aacgccatgc
     7981 tgaaacagca gcaagaagtc gcgaaactgc gcgaattcat ccagcgtgcg tacgatctgg
     8041 gcgctgacgt tcgtcagaaa gttgacctga gtaccttcag cgatgaagaa gttatgcgtc
     8101 tggctgaaaa cctgcgcaaa ggtatgccaa tcgcaacgcc ggtgttcgac ggtgcgaaag
     8161 aagcagaaat taaagagctg ctgaaacttg gcgacctgcc gacttccggt cagatccgcc
     8221 tgtacgatgg tcgcactggt gaacagttcg agcgtccggt aaccgttggt tacatgtaca
     8281 tgctgaaact gaaccacctg gtcgacgaca agatgcacgc gcgttccacc ggttcttaca
     8341 gcctggttac tcagcagccg ctgggtggta aggcacagtt cggtggtcag cgtttcgggg
     8401 agatggaagt gtgggcgctg gaagcatacg gcgcagcata caccctgcag gaaatgctca
     8461 ctgttaagtc tgatgacgtg aacggtcgta ccaagatgta taaaaacatc gtggacggca
     8521 accatcagat ggagccgggc atgccagaat ccttcaacgt attgttgaaa gagattcgtt
     8581 cgctgggtat caacatcgaa ctggaagacg agtaattctc gctcaaacag gtcactgctg
     8641 tcggggtaaa acccggcagc ggattgtgct aactccgacg ggagcaaatc cgtgaaagat
     8701 ttattaaagt ttctgaaagc gcagactaaa accgaagagt ttgatgcgat caaaattgct
     8761 ctggcttcgc cagacatgat ccgttcatgg tctttcggtg aagttaaaaa gccggaaacc
     8821 atcaactacc gtacgttcaa accagaacgt gacggccttt tctgcgcccg tatctttggg
     8881 ccggtaaaag attacgagtg cctgtgcggt aagtacaagc gcctgaaaca ccgtggcgtc
     8941 atctgtgaga agtgcggcgt tgaagtgacc cagactaaag tacgccgtga gcgtatgggc
     9001 cacatcgaac tggcttcccc gactgcgcac atctggttcc tgaaatcgct gccgtcccgt
     9061 atcggtctgc tgctcgatat gccgctgcgc gatatcgaac gcgtactgta ctttgaatcc
     9121 tatgtggtta tcgaaggcgg tatgaccaac ctggaacgtc agcagatcct gactgaagag
     9181 cagtatctgg acgcgctgga agagttcggt gacgaattcg acgcgaagat gggtgcggaa
     9241 gcaatccagg ctctgctgaa gagcatggat ctggagcaag agtgcgaaca gctgcgtgaa
     9301 gagctgaacg aaaccaactc cgaaaccaag cgtaaaaagc tgaccaagcg tatcaaactg
     9361 ctggaagcgt tcgttcagtc tggtaacaaa ccagagtgga tgatcctgac cgttctgccg
     9421 gtactgccgc cagatctgcg tccgctggtt ccgctggatg gtggtcgttt cgcgacttct
     9481 gacctgaacg atctgtatcg tcgcgtcatt aaccgtaaca accgtctgaa acgtctgctg
     9541 gatctggctg cgccggacat catcgtacgt aacgaaaaac gtatgctgca ggaagcggta
     9601 gacgccctgc tggataacgg tcgtcgcggt cgtgcgatca ccggttctaa caagcgtcct
     9661 ctgaaatctt tggccgacat gatcaaaggt aaacagggtc gtttccgtca gaacctgctc
     9721 ggtaagcgtg ttgactactc cggtcgttct gtaatcaccg taggtccata cctgcgtctg
     9781 catcagtgcg gtctgccgaa gaaaatggcg ctggagctgt tcaaaccgtt catctacggc
     9841 aagctggaac tgcgtggtct tgctaccacc attaaagctg cgaagaaaat ggttgagcgc
     9901 gaagaagctg tcgtttggga tatcctggac gaagttatcc gcgaacaccc ggtactgctg
     9961 aaccgtgcac cgactctgca ccgtctgggt atccaggcat ttgaaccggt actgatcgaa
    10021 ggtaaagcta tccagctgca cccgctggtt tgtgcggcat ataacgccga cttcgatggt
    10081 gaccagatgg ctgttcacgt accgctgacg ctggaagccc agctggaagc gcgtgcgctg
    10141 atgatgtcta ccaacaacat cctgtccccg gcgaacggcg aaccaatcat cgttccgtct
    10201 caggacgttg tactgggtct gtactacatg acccgtgact gtgttaacgc caaaggcgaa
    10261 ggcatggtgc tgactggccc gaaagaagca gaacgtctgt atcgctctgg tctggcttct
    10321 ctgcatgcgc gcgttaaagt gcgtatcacc gagtatgaaa aagatgctaa cggtgaatta
    10381 gtagcgaaaa ccagcctgaa agacacgact gttggccgtg ccattctgtg gatgattgta
    10441 ccgaaaggtc tgccttactc catcgtcaac caggcgctgg gtaaaaaagc aatctccaaa
    10501 atgctgaaca cctgctaccg cattctcggt ctgaaaccga ccgttatttt tgcggaccag
    10561 atcatgtaca ccggcttcgc ctatgcagcg cgttctggtg catctgttgg catcgatgac
    10621 atggtcatcc cggagaagaa acacgaaatc atctccgagg cagaagcaga agttgctgaa
    10681 attcaggagc agttccagtc tggtctggta actgcgggcg aacgctacaa caaagttatc
    10741 gatatctggg ctgcggcgaa cgatcgtgta tccaaagcga tgatggataa cctgcaaact
    10801 gaaaccgtta ttaaccgtga cggtcaggaa gagaagcagg tttccttcaa cagcatctac
    10861 atgatggccg actccggtgc gcgtggttct gcggcacaga ttcgtcagct tgctggtatg
    10921 cgtggtctga tggcaaagcc ggatggctcc atcatcgaaa cgccaatcac cgcgaacttc
    10981 cgtgaaggtc tgaacgtact ccagtacttc atctccaccc acggtgctcg taaaggtctg
    11041 gcggataccg cactgaaaac tgcgaactcc ggttacctga ctcgtcgtct ggttgacgtg
    11101 gcgcaggacc tggtggttac cgaagacgat tgtggtaccc atgaaggtat catgatgact
    11161 ccggttatcg aggggggtga cgttaaagag ccgctgcgcg atcgcgtact gggtcgtgta
    11221 actgctgaag acgttctgaa gccgggtact gctgatattc tcgttccgcg caacacgctg
    11281 ctgcacgaac agtggtgtga cctgctggaa gagaactctg tcgacgcggt aaaagtacgt
    11341 tctgttgtat cttgtgacac cgactttggt gtatgtgcgc actgctacgg tcgtgacctg
    11401 gcgcgtggcc acatcatcaa caaaggtgaa gcaatcggtg ttatcgcggc acagtccatc
    11461 ggtgaaccgg gtacacagct gaccatgcgt acgttccaca tcggtggtgc ggcatctcgt
    11521 gcggctgctg aatccagcat ccaggtgaaa aacaaaggta gcatcaagct cagcaacgtg
    11581 aagtcggttg tgaactccag cggtaaactg gttatcactt cccgtaacac cgaactgaaa
    11641 ctgatcgacg aattcggtcg taccaaagaa agctacaaag tgccttacgg tgcggtactg
    11701 gcgaaaggcg atggcgaaca ggttgctggc ggcgaaaccg ttgcaaactg ggacccgcac
    11761 accatgccgg ttatcaccga agtaagcggt tttgtacgct ttactgacat gatcgacggc
    11821 cagaccatta ctcgtcagac cgacgaactg accggtctgt cttcgctggt ggttctggat
    11881 tccgcagaac gtaccgcagg tggtaaagat ctgcgtccgg cactgaaaat cgttgatgct
    11941 cagggtaacg acgttctgat cccaggtacc gatatgcctg cgcagtactt cctgccgggt
    12001 aaagcgattg ttcagctgga agatggcgta cagatcagct ctggtgacac cttggcgcgt
    12061 attccgcagg aatccggcgg taccaaggac atcaccggtg gtctgccgcg cgttgcggac
    12121 ctgttcgaag ctcgtcgtcc gaaagagccg gcaatcctgg ctgaaatcag cggtatcgtt
    12181 tccttcggta aagaaaccaa aggtaaacgt cgtctggtta tcaccccggt agacggtagc
    12241 gatccgtacg aagagatgat tccgaaatgg cgtcagctca acgtgttcga aggtgaacgt
    12301 gtagaacgtg gtgacgtaat ttccgacggt ccggaagcgc cgcacgacat tctgcgtctg
    12361 cgtggtgttc atgctgtgac tcgttacatc gttaacgaag tacaggacgt ataccgtctg
    12421 cagggcgtaa agattaacga taaacacatc gaagttatcg ttcgtcagat gctgcgtaaa
    12481 gctaccatcg ttaacgcggg cagctccgac ttcctggaag gcgaacaggt tgaatactct
    12541 cgcgtcaaga tcgcaaaccg cgaactggaa gcgaacggca aagtgggtgc aacttactcc
    12601 cgcgatctgc tgggtatcac caaagcgtct ctggcaaccg agtccttcat ctccgcggca
    12661 tcgttccagg agaccactcg cgtgctgacc gaagcagccg ttgcgggcaa acgcgacgaa
    12721 ctgcgcggcc tgaaagagaa cgttatcgtg ggtcgtctga tcccggcagg taccggttac
    12781 gcgtaccacc aggatcgtat gcgtcgccgt gctgcgggtg aagctccggc tgcaccgcag
    12841 gtgactgcag aagacgcatc tgccagcctg gcagaactgc tgaacgcagg tctgggcggt
    12901 tctgataacg agtaatcgtt aatccgcaaa taacgtaaaa acccgcttcg gcgggttttt
    12961 ttatgggtgt tattcagagg gcaaattctt cacggaaaat attaagaaag gcagggcgaa
    13021 acgaggaaga agcttttcgc cctggacaac attcctgctg acggcactac cataaaaaaa
    13081 cacaacgctt tcaacaacct tgcgtttttc atcttccgta gcggctcgga ataaatttac
    13141 gttcttacat tccgtctcat agtctttgcg aggcgcgtcc cagatagctg tcccagtctt
    13201 tccataccgg ctgcaaaccc tgagcggtta acacggcagc aaccgcttcc ggtctgcgat
    13261 cgtcgtgcgg tgagaactgt tccaactcgg ggtgattatc ggcatagcca cctggctgcg
    13321 ttttcgagaa ggcgctgacg ttattgatcg ccagcggaat aacgcgatcg cgaaaccacg
    13381 gtgattcccg cgtggagagt gacagttcaa tctccggtgc aagcagtcgg aaggcgcaga
    13441 tggtttgcac taactggcgt tcatccataa tcgacgcagg ctcaatgccg ccagtacacg
    13501 ggcgcaggcg cggaaaggag acagagtaac ggctttgcca gtaatgctgt tgcagccata
    13561 gcaaatgttc ggcaaccata tagcagtcaa cgcgccagtt gtcggaaagg ccaattagcg
    13621 cgccgaggcc tatcttatca atccccgcac gccccagccg atccggcgtt tccagccgcc
    13681 agaagaagtc ctgttttttg cctttcaaat gatggcgggt ataagtcgcc tcgtgatatg
    13741 tctcctgata aaccatcacc ccatccagac caagttgctt taactcggcg tattccgtct
    13801 ccgccagcgg ttgcacttcc atttgtagtg aagagaactg ttcacgcagg gcggggagat
    13861 gacgacgaaa gtaatccatc cccactttcg cctgatgttc accagtgact aatagcagat
    13921 gttcaaagcc catctcccgt atagcggcgc tttccctggc aatatccgct tcatccagcg
    13981 ttttgcgctt gatgcgatta ctcatggaaa atccgcagta cgtgcagtcg ttagcgcaaa
    14041 gattggaaag ataaagcggg acgtagaaac taactgtgtt gccaaatcgc tgacgggtca
    14101 gacgctgcgc ctgttgggcc agttgttcca gatagccact ggcggcaggc gataacagcg
    14161 ccatcatgtc gtcgcgggtg agttgcgagg catttagcgc ccgctctacg tcagcagccg
    14221 ttttgccgtt gatacgcagg cggatgtcgt cccagtccag ttgtcgccag cgattgctga
    14281 aggttttcat gccgatgcct ccagaaatcc ggtcagcggg ctggtggcat gagcaaaata
    14341 actgcggctg cccggtccgg actgacgtgc cagcaggcct acttctaccg ccagacgaaa
    14401 tgccttcgcc atgttgacgg gatcgtccgc gacggcaatc gccgtattca ctaacaccgc
    14461 gtcggccccc atttccagcg cctgcgtggc atggctggga acgccgatgc cagcatcgac
    14521 aaccaccgga actgtggcct gctggataat aatctccagc atggcacggg tttccagtcc
    14581 ctgattcgag ccaatcggcg cgccgagcgg catcaccgct gcacagccga cttcttccag
    14641 acgtttacac aataccggat cggccccgca gtaaggcagc acgacaaatc cctgttgtac
    14701 cagcgtttcg gcggctttca gggtttcgat gggatcgggc aacagccagc gggcgtcagg
    14761 gtgaatctcc aattttagcc agtttgtgcc taacgcttca cgtgccagat gggcggcgaa
    14821 aatggcttct tccgctgttt tcgccccgga agtattcggc agcagggcca cacccgccgc
    14881 gataagcggt tcgaggatag cgtcgttgtg ctggcgcaag tcgacacgtt tcatcgccag
    14941 tgtcaccagc tggctgccgg aagcgcggat cgcctccacc atcagttgtg aagaagcgaa
    15001 tttccctgtg ccggtaaaca gatgtgaatc aaacgttttg tccgcaatac gtaacatctc
    15061 aaccccctgc aataacctga aaaagcagga tctggtcgcc atcctgcacg atatgttgcg
    15121 tccactgctc acgcgggacg atttgctgat taatcgccag agccgcgccc gcttgtcgtt
    15181 ggtcgagttg ctccagtagt tcgtgaacag tttgcccggc ggcgcactgc atcggttgat
    15241 cgttaaacag gatctgcatt gcttcctccg cataccgggc aaccactggc gcggcgcaac
    15301 gccaggctgc gccactggct cgacttaccg tcgaacagtc ggagttctcc cgcaggcgtc
    15361 tctataccgc ttaataactt aatggcttcc agtgcctgca aagtgcccat caccccgacc
    15421 accgggccaa ccacgcccgc cgtgcggcag ttgcgttctg gctgctggtt atctggccac
    15481 aggcagcggt aacacccctg ctcccagggc ggtgtcagta ccatcaactg accgccaaat
    15541 ccgaccgcgc tggcggtgat aagcggcgtg ttgagtgcca cgcaggcggc attaatctcc
    15601 tggcgagtcg ccatattgtc ggtacagtcg agcaccacat cggcttgtgc aaccgcatct
    15661 ttcaacgcct cacccgttaa ccgttgttgt aatgctgtca gttgaatgtc gggattcaac
    15721 tgtgtcagtc gctgttggct gacctgcgat ttcgggctgt tgatatcttc agtggtaaag
    15781 aggatttgtc gttgcagatt gcttaaatgc acatcgtcgt catctgccag taccagcgtc
    15841 ccgacgccag cccctgccag gtatagcgca gcaggtgtac ccagcccgcc aagaccaata
    15901 atcagcacct ggctgtcgag cagtttttgc tgcccgtcaa gagcgatatc gtcgagcaga
    15961 atttggcggc tataacgcat aaagtcacgg tcattcatcg ccaactcctg caatttccag
    16021 caactgtgcc gttgccaaac gccagtctgc ggcttgagta atggcgctga cgacggcgat
    16081 actgccgaca cccgttgcta tcaccgcagg cgcgcgtgcc agactgatac cgccaatcgc
    16141 cacggtggga taatccgcca ggcgctcaac atgccgtgcc aactgttcca gcccctgcgg
    16201 tgcagaaggc atctgtttgg tttgcgtcgg gaacacatgt cccagcgcga tataagaggg
    16261 gcgtgctgcc agcgcgacgt cgatttccat atcgtcatgt gtcgaaacgc ccagccgcag
    16321 gcctgccgcg cggatggcat tgagatcggt ggcttgcaaa tcttcctgcc ccaaatggac
    16381 gccatacgcc tgatgcttga tcgcgagccg ccagtaatcg ttgataaaca atcgcgcgtt
    16441 atagcggcgg cccagcgcaa ttgccgccac gacatcggct tccacctctt catcgcgccg
    16501 atctttgatg cgtagctgga gagtacgtac gcctgcatcc aacagacgtt cgatccactg
    16561 tacgctgtcc accaccgggt acagtcctaa acgaaaaggt acaggaggaa aatcaggctg
    16621 atacatcacg cttcctcctt atgcaggtag atttctccgc ctctggcacg gaagttctcc
    16681 gacatatccg ccattcccac ttcaatggtt tgcgcggcgg cgtaatcgcg tacttcctgg
    16741 ctgattttca tcgagcagaa ttttggccca cacatggagc aaaaatgggc gactttgccg
    16801 gactcttggg gcagggtttc atcgtgataa gcgcgggcgg taaacgggtc gagggccaga
    16861 ttaaactggt cttcccagcg aaattcgaag cgggctttcg acatggcgtt atcgcgaatt
    16921 tgcgcgcccg gatgcccttt tgccaggtca gcagcgtggg cggcaatctt ataagtgata
    16981 agcccctgct taacatcttc tttattgggc agacccagat gctcttttgg cgttacgtaa
    17041 cagagcatcg cgcagccaaa ccagccaatc atcgccgcac caatccccga cgtgaagtgg
    17101 tcatagcccg gtgcaatatc ggtagttagc ggccccagag tgtaaaacgg cgcttcgtgg
    17161 cagtgctcta actcctcggt catattgcgg cggatcatct gcatcggcac atggcctggg
    17221 ccttcaatca tcacctgtac gtcatattcc caggcgattt tggtcagttc gcccagcgta
    17281 tgcaattcgg caaactgcgc ttcatcgttg gcgtcctgaa tagaaccggg gcgcagaccg
    17341 tcgcccagcg acagcgaaac gtcataagcg gcacagattt cacaaatttc gcggaagtgt
    17401 tgatagagga aattttcctg atgatgggag aggcaccatt tcgccataat cgaaccgccg
    17461 cgagagacga taccggtcag gcgtttcgcg gtcatcggca catagcgcag cagtacgccc
    17521 gcatggatag tgaagtaatc cacaccttgc tcggcctgtt ccagcagcgt gtcgcggaac
    17581 gcttcccagg taagatcttc ggcgatcccg ttaaccttct ccagcgcctg gtagatcggc
    17641 actgtaccga tcggcaccgg actgttacgc aaaatccact cgcgggtttc gtgaatatag
    17701 cgaccggtgg agagatccat caccgtatcc gctccccagc gcgtggacca taccagcttt
    17761 tccacttctt cttcgatgga agaggtgacc gccgagttgc cgatattggc gttaactttt
    17821 accaggaagt tacggccaat aatcatcggc tccgattccg gatgattaat gttggccggg
    17881 ataatcgcac gtccggcagc aacttcatca cggacaaatt ccgcagtgat attttccggc
    17941 agatgtgcgc caaagctcat tcccggatgc tggtggcgta aaacctcgct acggatgcgc
    18001 tcgcggccca tattctcgcg gatggcgatg aattccatgt ccggcgtgat gatgccctgg
    18061 cgggcgtagt gcagttgggt gacacggcgt cctgttttgg cgcgttttgg cgttaataag
    18121 ccgctaaaac gtagctcgtc gagaccatca tctgccagcc gcgttctggt gtaatcggaa
    18181 ctgcgcacgg taagttcttc ggtatcggcg cgcgcatcga tccacggctg gcgtagtttt
    18241 gccagccctt gctgcacgtt aatggcgatc tgcggatcac catacgggcc ggaggtgtcg
    18301 tagaccggaa tcgcttcgtt ttcttcgtac tgcggctgtt ctttgctacc gccaattagc
    18361 gtcgggctaa gctggatctc acgcatcggc acgcgcacgc cggggtgtgt gccagtgata
    18421 taaatgcgtt ttgagttggg aaaggcggtg ccttccaggg tgtcgataaa atgttgggcc
    18481 cgggcgcgtt gttcgcggcg ggtcagtttt gttgcagaca tagctcattc caaaaagtta
    18541 aggacgtggc ttgtcagacg acggatgaag caagagacga tcgccgcaaa ggcgatgcga
    18601 tagcagatta actcttgttc ccttcgcagg tattagcctg atcaggttcc gcggatcccg
    18661 aataaacggt ctcagccagt taaggcactc cgacaagaaa ttagccccca caaacggggc
    18721 gttgaatgta aattacgcgt taacagcgca gaactcaagc aggatgtttg acgcgggcgg
    18781 cgtcaagcac cagcagaatc agcttatctt ccagcacaaa gcgcgcttcc agcgcctcgc
    18841 cgatgtcaga taaaacctgt tgaaactcaa ggtaattatc atgatcgatg gcggtttcca
    18901 ggctggaatc gtagtaatcc ataatctgtt gggtattggc ttcgagttgc ggccaaatct
    18961 tcgcggcgcg cgcgagttgc ccgttgcctt ccagcttatg aagaatgcgc tcataaatac
    19021 tgaaatgtcc ggcagacaag taatcgacca ggctctgaca aaaatcatca agggcttttt
    19081 cgtttagcct catgtacgat tctttgccag gcttaatgcc aaccagattg tagtaagcca
    19141 cgagcagatg cttacgtaca tgtagccagc gatcaaccag tttgttactt cctctgacgc
    19201 gctccgtcag gttatcgagc tggttaagca tgattgactc cgcaagtttg tattcaaaaa
    19261 ctgctcagtg agaaatgtaa aaaccatgtt aaacatgcca gtgatgcaaa ggtagtgcaa
    19321 gagctatgga tcgtataatt gaaaaattag atcacggctg gtgggtcgtc agccatgaac
    19381 aaaaattatg gttgccgaag ggagaattgc catatggcga agcggcaaat ttcgatcttg
    19441 tgggtcagcg cgcactacaa atcggcgaat ggcaggggga acctgtttgg ttaatacaac
    19501 agcagcggcg tcacgatatg gggtcggtac gtcaggtcat tgatctcgat gttgggctgt
    19561 ttcaactggc cggacgtggc gtgcaactgg cggagtttta ccgatcgcat aaatactgtg
    19621 gttactgcgg gcatgaaatg tatccgagca aaaccgaatg ggcgatgctg tgtagccatt
    19681 gccgtgagcg ttactacccg caaatcgccc cctgcattat tgttgccatc cgtcgcgatg
    19741 attcgctcct cctcgcccag catacccgcc atcgtaacgg tgtccataca gtacttgccg
    19801 gattcgtcga agtgggcgaa accctcgagc aggcagtcgc gcgggaagtg atggaagaga
    19861 gcggaattaa agttaaaaac ttgcgttacg tgacttctca gccgtggccg tttcctcagt
    19921 ctttaatgac cgcgtttatg gcggaatatg acagcggcga catcgtgatc gacccgaaag
    19981 aattgctcga ggcgaactgg tatcgctatg acgatttgcc gttactcccg ccgcccggca
    20041 ccgtagcgcg ccgtctgata gaagatactg tggcgatgtg tcgggcagag tatgagtgat
    20101 gatacactga ccgcctgacg cactaaggaa cagccaaaat gaccgaactt aaaaacgatc
    20161 gttatctgcg ggcgctgctg cgccagcccg ttgatgtcac tccagtatgg atgatgcgcc
    20221 aggcgggtcg ctatctaccg gaatataaag ccacgcgcgc ccaggcgggc gattttatgt
    20281 cgctgtgcaa aaacgccgag ctggcgtgcg aagtgacttt gcaaccgctg cgtcgctacc
    20341 cgctggatgc ggcgatcctc ttttccgata tcctcaccgt gccggacgcg atggggttag
    20401 ggctctattt tgaagccgga gaaggtccgc gttttacctc gccagtcacc tgcaaagccg
    20461 acgtcgataa actgccaatt ccggacccgg aagatgagct gggttacgtg atgaacgcgg
    20521 tgcgtaccat tcgtcacgaa ctgaaaggcg aagtgccgct gattggtttt tccggcagcc
    20581 cgtggacgct ggcaacctac atggtggaag gcggcagcag caaagccttc accgtgatca
    20641 aaaaaatgat gtatgccgat ccgcaggcgc tgcacgctct gctcgataaa ctggcgaaaa
    20701 gcgtcacttt gtatctgaat gcgcagatta aagccggtgc tcaggcagtg atgattttcg
    20761 acacctgggg cggcgtgctt accgggcgcg attatcaaca gttctcgctc tattacatgc
    20821 ataaaattgt tgatggttta ctgcgtgaaa acgacggtcg ccgcgtgcca gtcacgctgt
    20881 ttaccaaagg cggcggacag tggctggaag ccatggctga aaccggttgc gatgcgctgg
    20941 gcctcgactg gacaacggac atcgccgatg cgcgccgccg tgtgggcaat aaagtcgcgt
    21001 tgcagggtaa tatggatccg tcgatgctgt acgcgccgcc tgcccgcatt gaagaagaag
    21061 tagcgactat acttgcaggt ttcggtcacg gcgaaggtca tgtctttaac cttggtcacg
    21121 gcattcatca ggatgtgccg ccagaacatg ctggcgtgtt cgtggaggca gtgcatcgac
    21181 tgtctgaaca gtatcaccgc taagagcctg atccatcagg ctattttatt tgccattttg
    21241 aggccgggca gtgctcaaaa tcctcacgta ctacgtgtac gctccggttt ttccgcgctg
    21301 tccggcctca aactgccttc aacaattacg cctgttgaac caggctctaa ggagcgatta
    21361 tggatctcgc gtcattacgc gctcaacaaa tcgaactggc ttcttctgtg atctgcgagg
    21421 atcgactcga taaagatcca ccggatctga tcgccggagc cgatgtcggg tttgagcagg
    21481 gcggagaagt gacgcgagcg gcgatggtgc tgctgaaata tccctcgctt gagctggtgg
    21541 agtataaagt tgcccgcatc gccaccacca tgccttacat tccaggtttt ctttccttcc
    21601 gcgaatatcc tgcgctgctg gcagcgtggg agatgctgtc gcaaaagccg gatttagtgt
    21661 ttgtcgatgg tcatgggatc tcgcatcctc gccgtcttgg cgtcgccagc cattttggct
    21721 tattggtgga tgtgccgacc attggcgtgg cgaaaaaacg gctctgcggt aaattcgaac
    21781 cgctctccag caaaccgggc gcgctggccc cactgatgga taaaggcgag cagctggcct
    21841 gggtctggcg cagcaaagcg cgctgtaacc cgttgtttat cgctaccggc catcgggtca
    21901 gcgtggacag cgcgctggcg tgggtacaac gctgcatgaa aggctatcgt ctgccggagc
    21961 caacgcgctg ggcggacgcg gtggcctcgg aacgtccggc gttcgtgcgc tatacagcaa
    22021 atcagcccta attcaggtaa actgcggata atttccgtat ttgagaactc aacatgttac
    22081 aaaacccaat tcatctgcgt ctggagcgcc tggaaagctg gcagcatgtc actttcatgg
    22141 cttgcttatg cgaacgcatg taccccaatt acgccatgtt ctgccagcaa accggttttg
    22201 gtgatgggca aatttaccgt cgtattctcg atctcatctg ggaaacgctg accgttaaag
    22261 acgcaaaagt aaatttcgac agccaactgg agaaatttga agaagcgatt ccttcagccg
    22321 acgatttcga tctgtacggc gtttatccgg caatcgatgc ctgcgtggcg ttaagtgaac
    22381 tggtccattc gcgtttgagt ggtgaaacgc tcgaacacgc ggtggaagtg agtaagacct
    22441 ccatcacgac cgttgcgatg ctggaaatga ctcaggctgg tcgcgaaatg ggcgatgaag
    22501 agcttaaaga aaacccagct gtagagcaag aatgggacat tcagtgggaa atattccgac
    22561 ttttagctga gtgcgaagaa cgcgatatcg agctgataaa aggccttagg gcagacctgc
    22621 gtgagtcggg tgagagcaat attggtataa tttttcagca ataagaccag aaaacgtgat
    22681 ttaacgcctg atttgtcgta cctggagtct tccctttcgc cccccgtctg gtctacattt
    22741 ggggggcgaa aaaaagtggc tatcggtgcg tgtatgcagg agagtgctat tctggcattt
    22801 ccgtcgcact cgatgcttag caagcgataa acacattgta aggataactt atgaacaaga
    22861 ctcaactgat tgatgtaatt gcagagaaag cagaactgtc caaaacccag gctaaagctg
    22921 ctctggagtc cactctggct gcaattactg agtctctgaa agaaggcgat gctgtacaac
    22981 tggttggttt cggtaccttc aaagtgaacc atcgcgctga gcgtactggc cgcaacccgc
    23041 agaccggtaa agaaatcaaa attgccgcag ctaacgtacc ggcatttgtt tctggcaagg
    23101 cactgaaaga cgcagttaag taagattgcg tggcagtgaa cagttttaac gaaggggtgg
    23161 tttcacccct tttgtctttc tggcgtcgat cattgatgct ggcaggtgcg ctgtttctca
    23221 ctgcctgtag ccacaactct tcacttcctc cctttaccgc cagtggattt gctgaagacc
    23281 agggcgcggt acgcatctgg cgaaaagaca gcggcgataa tgtgcatctg cttgccgtgt
    23341 ttagcccgtg gcgcagtggc gataccacga cgcgagagta tcgctggcag ggcgataacc
    23401 tcacgctcat caatatcaat gtttacagca aaccgccggt gaacattcgc gcgcgttttg
    23461 acgatcgcgg cgatctgagc tttatgcaac gtgaatccga tggggaaaag cagcagcttt
    23521 ctaacgacca aatcgattta taccgttatc gtgctgatca gatccgccag attagcgatg
    23581 ccttacgtca ggggagagtc gtgctgcgcc aggggcgctg gcatgcgatg gaacagaccg
    23641 tgaccacctg cgaagggcaa accattaaac ctgatttaga ttcgcaggcg atagcgcata
    23701 tcgagcgccg ccagagccgc tcttctgttg atgtcagcgt ggcatggctg gaagcgcccg
    23761 aaggttcgca attactgtta gtggcaaact ctgatttctg tcgctggcaa cccaacgaga
    23821 aaacgttctg atttaccagt ggcccatacc catatgaccg ccaccaccgc agccgccgta
    23881 gcccatgccc attccggctc cgcgcggaat acccgcttca gccatcgcga tatctcgttt
    23941 cacccgtaac tcatctaacg actgacgcaa attctccatc tctttggcga ccgcgttaat
    24001 tttgctgcta tccggtgggt tcgcggctaa cagggcattg tattcataac gcttcgtcac
    24061 cagttgctgt tgcagtgcgc tgctttgagc gtaaaagtca ttatggattt tctgccacgc
    24121 tgtctgttgt tcgctggtca aaggcgcggc attttgctgc cacataccgt gtccgccgtg
    24181 agcaaatgca gatgtcgatc ccatcgccat tgctgaaagc gccatcatta ccagggcaat
    24241 tttcgtgttc cgtttcatgg ttaatcctcc agtggttgtc ttacttcggg tattgcatct
    24301 tccgtgccaa cgatgaaacg ctgatatgac gggtaatctg gcatgataaa cgagtaaaaa
    24361 tgactcgcct gctgcgggta gcgagtcatt tttactcatt gaaacttaag cctttgtgtt
    24421 acagcgcagg gtaagcgctg ataaaagatg gcatgatttc tgctgtcaga aagggatgag
    24481 caggcaaaga agaagatgcg ttttatgcaa cgttctaaag actccttagc taaatggtta
    24541 agcgcgatcc tccccgtggt cattgttggg ctggtagggt tgtttgcggt gacggtgatt
    24601 cgcgattatg ggcgcgagac tgccgccgcc agacaaacgc tgctggaaaa aggcagtgta
    24661 cttattcgcg ctcttgaatc cggctcgcgc gtcggcatgg ggatgcgcat gcatcatgcg
    24721 cagcagcagg ccttactgga agaaatggcc gggcagcctg gagtacgttg gtttgcggtc
    24781 acggatgaac aaggaacaat cgtgatgcat agcaactccg gcatggtggg aaaacagctt
    24841 tattcccctc aggaaatgca gcagttacat ccgggagatg aagaagcgtg gcggcggatc
    24901 gatagcgcag acggcgagcc tgttctggaa atttatcgcc agtttcaacc gatgtttgct
    24961 gctggaatgt accggatgcg ccatatgcag cagtatgccg cgacaccaca agcaattttc
    25021 atcgctttcg acgccagtaa tattgtgagt gccgaagatc gtgagcagag aaacaccctg
    25081 attattctct tcgccctggc gacggtcttg ctggcaagcg tattgtcatt cttctggtat
    25141 cgccgctatc tgcgctcgcg ccagcttcta caagatgaaa tgaagcgcaa agagaagctg
    25201 gtggcgctgg ggcatcttgc ggcaggcgtt gcccacgaaa tccgtaaccc actttcctcg
    25261 attaaaggac tggcgaaata ctttgccgag cgcgcgcctg cagggggaga agcgcatcaa
    25321 ctggcgcagg tgatggcgaa agaggccgac cgtttaaacc gcgtggtaag cgagttgctg
    25381 gaactggtta agccaacgca tctggctttg caggcggtgg atctcaacac gctgattaac
    25441 cactcattac agctggtaag ccaggatgca aacagccggg agatccagtt acgctttacc
    25501 gccaacgaca cattaccgga aattcaggcc gatccggaca ggctgactca ggtcctgttg
    25561 aatctctatc tcaatgctat tcaggcgatt ggtcagcatg gcgtgattag cgtgacggcc
    25621 agcgaaagcg gcgcgggcgt gaaaatcagc gttaccgaca gcggtaaggg aattgcggca
    25681 gatcagcttg aagccatctt cactccgtac ttcaccacca aagccgaagg caccggattg
    25741 gggctggcgg tcgtgcataa tattgttgaa caacacggtg gtacaattca ggtcgcaagc
    25801 caggagggaa aaggctcaac gttcaccctc tggcttccgg tcaatattac gcgtaaggac
    25861 ccacaaggat gacgcacgat aatatcgata ttctggtggt ggatgatgac attagccact
    25921 gcactatttt gcaggcttta ctgcgcggct ggggctataa cgtcgcgctg gcgaacagcg
    25981 ggcgacaggc gctggagcag gtgcgggaac aggtttttga tcttgtgctt tgcgatgtgc
    26041 gaatggcgga gatggacggc atcgccacgc tgaaagagat caaagcgtta aacccggcaa
    26101 ttccggtgct gattatgact gcgtactcca gcgtcgagac ggcggtagag gcactgaaaa
    26161 ctggggcgca ggattatctc atcaagccgc tggatttcga taacctacag gcgacgctgg
    26221 aaaaggcgct cgcgcatacg cacagtattg atgctgaaac acctgcggtg actgccagcc
    26281 agttcggtat ggtcggtaaa agcccggcga tgcaacacct gctcagtgaa atcgccctcg
    26341 tcgcgccatc ggaagccacg gtgctgatcc acggcgattc cggcaccggt aaagagctgg
    26401 tcgccagggc gattcacgcc agtagcgcac gtagcgaaaa accgctggta acgctcaact
    26461 gtgcggcact caacgaatcc ttgctggaat ctgaattgtt cggtcacgaa aaaggggcgt
    26521 ttactggagc cgataaacgc cgggaagggc gctttgttga ggcggacggc ggcacgctgt
    26581 ttctcgatga aattggcgat atctcgccga tgatgcaggt gcgtctgctg cgtgcgattc
    26641 aggagcgcga agttcagcgt gtcggtagca accagactat ctcggttgat gtccggctga
    26701 ttgcggcgac ccatcgcgat cttgccgcag aggtgaatgc cgggcgtttt cgccaggatc
    26761 tctactatcg cctgaatgtg gtggcgattg aagtaccatc gctgcggcaa cggcgggaag
    26821 atatccctct gctggctggc cattttcttc agcgctttgc cgagcgtaat cgcaaggcgg
    26881 taaaaggctt tacgccacag gcgatggatt tgctgatcca ctacgactgg ccgggaaata
    26941 ttcgtgaact ggaaaacgcg gtggaacggg cggtggtgct gctgaccggg gaatatattt
    27001 ccgaacgcga gctgccgctg gcgattgcca gtacgccgat cccgctgggg caaagtcagg
    27061 atattcagcc gctggtggaa gtggaaaaag aggtgattct ggcggcgctg gagaaaacgg
    27121 gcggcaacaa aaccgaagcc gcccgtcagt tagggatcac gcgcaaaacg ctattggcaa
    27181 aactgtcgcg ttagttctgc tcgcgttcga tggcgcgcca accgatatct ttccggcaga
    27241 agcaatcatc ccagtggata tcggtcatta aggcgtaggc gcgtttctgc gcttctgcca
    27301 cggtatgacc cagcgcggtg acgcacagta cgcgcccgcc gctggttacc acctgctcgt
    27361 catccgccag ttttgtgccc gcgtggaaca ctttgccgtc agccacttct tccagcggca
    27421 ggccgtggat cacatcgccg gtgcggtaat cgcccggata tccacccgca gccatcacca
    27481 cgctgagaga agcacgttca tcccactctg acgttttctc gtccagtttg ccttcacagg
    27541 ccgccaggca gagctcaacc agatcggact tcatgcgcag cataatcggc tgggtttccg
    27601 gatcgccaaa gcggcagtta aattcaataa ccttcggatt gccctgtttg tcgatcatca
    27661 ggcccgcata gagaaaaccg gtgtaggtgt tgccttccgc cgccatgcct ttcacggttg
    27721 gccagatgat acgttccatg gtgcgctgat gaacttcatc ggttactacc ggcgcggggg
    27781 agtaagcgcc catcccgccg gtgtttggtc cgttatcttt atcgcctacg cgtttgtgat
    27841 cctggctggt agccatcggc agcacatgct cgccgtccac catcacgata aagctcgctt
    27901 cttcgccatc gaggaactct tcgataacga tgcgatgacc cgcgtcgcca aaagcgttgc
    27961 ccgccagcat atcgtgaaca gccgcttccg cttcttccag cgtcatcgcc acgataacgc
    28021 ctttcccggc agccagaccg tccgctttaa tgacgattgg cgcgcctttc tcacgcagat
    28081 acaccagcgc aggttctacc tcggtgaagt tctggtattc cgccgtaggg atcttatggc
    28141 gggccaggaa atctttggta aacgcttttg agccttccag ttgggccgca cctgcggttg
    28201 ggccgaagat tttcagcccg gcggcgcgga aggtatcgac cacgcctttc accagcggcg
    28261 cttccgggcc gacgatggtc agatcaatct tttcgttttg tgcgaaatcc aacagcgccg
    28321 ggatatcggt cacgccgata gcaacgtttt gcagcgcggg ttccagcgca gtgcctgcat
    28381 tacccggagc aacaaaaaca gtctcaacca gcggcgactg ggccgctttc caggccagcg
    28441 cgtgctcgcg cccgccgtta ccaatcacta atactttcat ctattgctcc attaatggcg
    28501 gaagtggcgc atgtcggtga agagcatcgc aataccgtgc tcgtcggcgg cggcaatcac
    28561 ttcgtcatca cggatggaac cgccaggctg gattacgcag gtcacgcccg cagcggcggc
    28621 gacatcaata ccgtcgcgga acgggaagaa cgcgtcagaa gccatcgagg aacctttcac
    28681 ttccaggcct tcatcggcag ctttaatacc ggcgattttc gcggagtaca cgcggctcat
    28741 ctggcccgcg ccaataccga tggtcatatt gtttttggca tagacgatag cattggattt
    28801 cacgaatttc gccactttcc agcagaacag cgcatcacgc agttcctgtt cggtcggctg
    28861 acgttgggtg acgacgcgca gttcttccgc accgaccatc cccaggtcac gatcctgaac
    28921 cagcagaccg ccgttcacgc gtttgaaatc aagacccgga acacgctcgc cccactgacc
    28981 gcaggtcaga acgcgtacgt tctgcttggc ggcggtgatt ttcagggctt cttcgctggc
    29041 ggaaggcgca ataatcactt cgacaaactg acgagaaatg atggcctgtg cggtttccgc
    29101 atccagctcg cggttaaagg caatgatgcc gccgaatgcg gaggttgggt cggttttgta
    29161 cgcgcgatcg taagcatcaa gaatggaatt gccgatagct acaccgcaag ggttggcgtg
    29221 cttcacaatc acacatgccg gctcggcgaa ctctttcacg cactccagcg ccgcatcggt
    29281 atcggcgatg ttgttataag agagggcttt accctgaacc tgggttgcgg tagcaacgga
    29341 ggcttctttc acattctctt ctatatagaa ggcagcctgc tggtggctgt tctcgccgta
    29401 acgcatatcc tgcttcttaa tgaagttcag gttcagcgtg cgtgggaagc gaccggcggc
    29461 ttctttgctt tcaccgtggt acgccggaac catgctgccg aagtagttgg caatcatgct
    29521 gtcgtaggcg gcagtgtgtt cgaaggcttt gatggcgagg tcgaaacggg ttgcaagcgt
    29581 cagcgatcct tcgttgtcat ccatctcttt aataatggcg tcgtagtcgc tgctcttcac
    29641 cacgattgcg acatctttat ggttcttagc ggcggagcgc accatggtcg ggccgccgat
    29701 atcgatgttc tcaaccgcat cttccagcga gcagccttca cgagcaacag tctgggcgaa
    29761 cggatacagg ttaacaacca ccatatcgat aggctggatc tgatgttctt ccataatggc
    29821 atcgtcctgg ccgcgacggc ccagaatgcc accatgtact ttcggatgca gggtcttcac
    29881 gcgtccatcc atcatctccg ggaaaccggt gtaatcggaa acttcggtta ccggcagacc
    29941 tttttctgct aacagacggg cagtgccccc tgtagacagc agctccacac cgcgtgcgga
    30001 aagtgcctgg gcgaattcga cgataccggc tttgtcagaa acactgagca gagcgcggcg
    30061 gactggacga cgttgttgca tggtaaatcc cctgggtttg actattacag agagcgttag
    30121 ctgaattttt cgcgaaaaac tcagctaacg cccctaacgg ggcatcctta tttttcgccc
    30181 gcattgtaac gaaaacgttt gcgcaacgct cgcgaatttt tctctttcaa tggtgatcac
    30241 aattttgaca gtggttaccg tgggaaaaat acagaaatta cattgatgat tgtggataac
    30301 tctgtgcgta aaaaggtata aagcggggtt ttgctgggga atgcagcagt cagtcatttt
    30361 tctgcaattt ttctattgcg gcctgcggag aactccctat aatgcgcctt catcgacacg
    30421 gcggatgtga atcacttcac acaaacagcc ggttcggttg aagaga
//
LOCUS       DAJTQY010000045        29636 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.045, whole genome shotgun sequence.
ACCESSION   DAJTQY010000045 DAJTQY010000000
VERSION     DAJTQY010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29636
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.045"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(<1..188)
                     /locus_tag="OO349_002078"
     CDS             complement(<1..188)
                     /locus_tag="OO349_002078"
                     /inference="COORDINATES: protein motif:HMM:NF017243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HCR5867146.1"
                     /translation="MRHFIYQDEKSHKFWTVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAAEKAELKLIAEKVKKG"
     gene            complement(329..2362)
                     /gene="metG"
                     /locus_tag="OO349_002079"
     CDS             complement(329..2362)
                     /gene="metG"
                     /locus_tag="OO349_002079"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HCR5867147.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPELYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKATTAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            2494..3603
                     /gene="apbC"
                     /locus_tag="OO349_002080"
     CDS             2494..3603
                     /gene="apbC"
                     /locus_tag="OO349_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HCR5867148.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            3865..4146
                     /locus_tag="OO349_002081"
     CDS             3865..4146
                     /locus_tag="OO349_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HCR5867149.1"
                     /translation="MNKYWLSGIIFLAYGLASPAVSSETATLTINGRISPPTCSMAMV
                     NSQPQQHCGQLTYNVDTRHQVSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
     gene            4439..4963
                     /locus_tag="OO349_002082"
     CDS             4439..4963
                     /locus_tag="OO349_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5867150.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLTDNSVQS
                     GDVLSSLVMRVAQD"
     gene            5044..5718
                     /locus_tag="OO349_002083"
     CDS             5044..5718
                     /locus_tag="OO349_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCR5867151.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLYIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNIKSNNANNWYLTIINDHGN
                     YISDKI"
     gene            5734..8214
                     /locus_tag="OO349_002084"
                     /pseudo
     CDS             5734..8214
                     /locus_tag="OO349_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707994.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
     gene            8230..9263
                     /locus_tag="OO349_002085"
                     /pseudo
     CDS             8230..9263
                     /locus_tag="OO349_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
     gene            complement(9345..9683)
                     /gene="rcnB"
                     /locus_tag="OO349_002086"
     CDS             complement(9345..9683)
                     /gene="rcnB"
                     /locus_tag="OO349_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HCR5867152.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(9902..10750)
                     /gene="rcnA"
                     /locus_tag="OO349_002087"
                     /pseudo
     CDS             complement(9902..10750)
                     /gene="rcnA"
                     /locus_tag="OO349_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
     gene            10872..11144
                     /gene="rcnR"
                     /locus_tag="OO349_002088"
     CDS             10872..11144
                     /gene="rcnR"
                     /locus_tag="OO349_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HCR5867153.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     regulatory      11236..11332
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            11367..12155
                     /gene="thiM"
                     /locus_tag="OO349_002089"
     CDS             11367..12155
                     /gene="thiM"
                     /locus_tag="OO349_002089"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HCR5867154.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTRWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     gene            12152..12952
                     /gene="thiD"
                     /locus_tag="OO349_002090"
     CDS             12152..12952
                     /gene="thiD"
                     /locus_tag="OO349_002090"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCR5867155.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKIWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            13017..13838
                     /locus_tag="OO349_002091"
     CDS             13017..13838
                     /locus_tag="OO349_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HCR5867156.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGLDVSRWQERIDWQRVAKMRDNDIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVSASVQARLFLQTVDFSQGDFPAVL
                     DVDERGKLSAKELRKRVSQWLKMVEKRTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTGMSCRHSLMGLKKRLK"
     gene            13886..14632
                     /locus_tag="OO349_002092"
     CDS             13886..14632
                     /locus_tag="OO349_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR5867157.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLCGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(14606..15571)
                     /locus_tag="OO349_002093"
     CDS             complement(14606..15571)
                     /locus_tag="OO349_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HCR5867158.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     SDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGNLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFCPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(15568..16571)
                     /locus_tag="OO349_002094"
                     /pseudo
     CDS             complement(15568..16571)
                     /locus_tag="OO349_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
     gene            complement(16568..17845)
                     /locus_tag="OO349_002095"
     CDS             complement(16568..17845)
                     /locus_tag="OO349_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5867159.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGAVLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQMLGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAICYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            18102..19154
                     /gene="fbaB"
                     /locus_tag="OO349_002096"
     CDS             18102..19154
                     /gene="fbaB"
                     /locus_tag="OO349_002096"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HCR5867160.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLVSVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            19462..20316
                     /gene="gatY"
                     /locus_tag="OO349_002097"
     CDS             19462..20316
                     /gene="gatY"
                     /locus_tag="OO349_002097"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HCR5867161.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEVDALYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPVLDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFLQSLKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            20345..21607
                     /gene="gatZ"
                     /locus_tag="OO349_002098"
     CDS             20345..21607
                     /gene="gatZ"
                     /locus_tag="OO349_002098"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HCR5867162.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCAGDTIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQALAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLQGVDIPLGMISQYLPKQFERIQS
                     GELSAMPHQLIMNKIYDVLRAYRYGCAE"
     gene            21617..22069
                     /gene="gatA"
                     /locus_tag="OO349_002099"
     CDS             21617..22069
                     /gene="gatA"
                     /locus_tag="OO349_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HCR5867163.1"
                     /translation="MTNLFVRSGISFVDRSEVLTYIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            22100..22384
                     /gene="gatB"
                     /locus_tag="OO349_002100"
     CDS             22100..22384
                     /gene="gatB"
                     /locus_tag="OO349_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HCR5867164.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            22388..23743
                     /locus_tag="OO349_002101"
     CDS             22388..23743
                     /locus_tag="OO349_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HCR5867165.1"
                     /translation="MFSEVMRYILDLGPTVMLPVVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     CDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRMIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQPAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFTKQEKAVLA
                     E"
     gene            23791..24831
                     /gene="gatD"
                     /locus_tag="OO349_002102"
     CDS             23791..24831
                     /gene="gatD"
                     /locus_tag="OO349_002102"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCR5867166.1"
                     /translation="MKSVVNDTDGIVRVAKSVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMPGKVLLIP"
     gene            24937..25710
                     /locus_tag="OO349_002103"
     CDS             24937..25710
                     /locus_tag="OO349_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR5867167.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIVTDDAVSEVDKLALQKTRVKLITV"
     gene            complement(25792..26691)
                     /gene="yegS"
                     /locus_tag="OO349_002104"
     CDS             complement(25792..26691)
                     /gene="yegS"
                     /locus_tag="OO349_002104"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HCR5867168.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARFVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            27097..27414
                     /locus_tag="OO349_002105"
     CDS             27097..27414
                     /locus_tag="OO349_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5867169.1"
                     /translation="MKKIAAISLISIFLISGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            complement(27756..29117)
                     /gene="yegQ"
                     /locus_tag="OO349_002106"
     CDS             complement(27756..29117)
                     /gene="yegQ"
                     /locus_tag="OO349_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HCR5867170.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIHNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(29264..29595)
                     /locus_tag="OO349_002107"
                     /pseudo
     CDS             complement(29264..29595)
                     /locus_tag="OO349_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
BASE COUNT         7700 a         6879 c         7477 g         7580 t
ORIGIN      
        1 cccttcttca ccttctccgc aatcagcttc agttccgctt tttccgccgc cgcagcatct
       61 gaaaaacttt ttatctgact ttgccctttg gtgccaactt ttccccaact gatatgcaac
      121 tcgtttccct gttgctccac cgtccagaat ttatgtgatt tttcgtcctg atagataaag
      181 tgtctcattc tgcatccctg taattttatc cagttgaatg cagatgctac cagtatttat
      241 acgggttaga gagagacaaa atcgcaagaa aacaacttta ggaatttata aagaaaaggc
      301 gctgtcgatg cagcgccttg aagggggatt atttcacctg atgacccggt ttagcaccgg
      361 catccgggct tagcaggaaa atatctttcc cgccaggacc ggcagccatc accatgcctt
      421 cagagatacc gaagcgcatt ttacgtggtg ccaggttagc caccataatg gtgtgacgac
      481 caatcagtgc ctgcggatcc gggtaagcag aacgaatacc ggagaagaca ttgcgttttt
      541 caccgccgag atccagcgtc aggcgcagca gtttgtcaga accttcaaca aactctgcgt
      601 tttcgatcag cgccacgcgc aggtcaactt tggcgaagtc gtcaaaggtg atggtttcct
      661 gaatcggatc atctgccagt gggccagtaa ctggcgcggt agtggctttc acttcttctt
      721 tagaggcttc caccagagct tcaacctgtt tcatatcgat gcggttatac agcgccttga
      781 acggattcac tttgtggccc agcagcggtt gctggatacc atcccaggtc agttccgtat
      841 tgaggaaggc ttctgcacgc tcggtcagtt tcggcagtac cggcttcagg taagtcatca
      901 gcacgcggaa caggttgatg cccattgagc aaatcgcctg caggtcagca tcgcgacctt
      961 cctgttttgc gactacccac ggagcctgct cgtcgacata gcggttagcc aggtcagcca
     1021 gtgccatgat ttcgcgcact gctttaccaa attcacggct ttcccacgct tcaccaatca
     1081 cttcagcggc atcggtaaag gttttgtaca gctcagggtc agccagctcg cttgccagca
     1141 caccgtcaaa acgcttgttg ataaagcccg cattacggga ggccaggtta accactttgt
     1201 tcacgatatc ggcattcaca cgctgaacga aatcttccag gttgagatcg atatcatcaa
     1261 tgcgcgaaga gagtttcgca gtgtagtagt aacgcaggct gtcagcgtca aaatgattca
     1321 gccaggtgct ggctttaata aaggtgccgc gagacttgga catcttcgcg ccgttcaccg
     1381 tcacatagcc atgaacaaac aggttggtcg gcttgcggaa gttgctgcct tccagcatgg
     1441 caggccagaa caggctgtgg aagtaaacga tatctttacc gatgaagtgg tacagctcgg
     1501 cggtggagtc tttcttccag tattcatcga agcttacgct gtcgccgcgc ttgtcgcaca
     1561 gattcttgaa agaacccatg tagccaatcg gtgcgtccag ccagacgtag aaatatttgc
     1621 ccggtgcgtt cggaatttca aaaccaaagt atggcgcgtc gcgggagata tcccactgtt
     1681 gcaggccgga ttcaaaccac tcctgcattt tgtttgccac ctgctcctgc aacgcaccgc
     1741 tgcgggtcca tgcctgcagc atttcgctga aagagggcag atcaaagaag aagtgttcag
     1801 aatcacgcat cactggtgta gcaccagaaa ccaccgattt cggctcgatc aattcagtcg
     1861 ggctgtaggt cgcgccgcag acttcgcagt tatcgccgta ttgatccggg gatttacatt
     1921 tcgggcaggt gcctttcaca aaacggtccg gcaggaacat gcctttttcc ggatcgtaca
     1981 gctgagagat ggtgcggttt ttaataaaac cgttttcttt caggcgagag tagataagtt
     2041 ctgacaactg gcggttctct tcgctgtgcg tcgagtgata gttgtcatag ctgatgttaa
     2101 agcctgcgaa atcagtctga tgctcctgac tcatttcgcc aatcatctgc tccggggtga
     2161 taccgagctg ctgagctttc agcatgatcg gtgtaccgtg ggcatcgtcg gcgcagatga
     2221 agttgacctc gtggccgcgc attcgctggt aacggaccca gacatcagcc tggatgtgct
     2281 ccagcatatg gccgaggtgg attgagccgt tagcgtacgg cagtgcgcac gtcaccagaa
     2341 ttttcttcgc gacttgagtc atagtaggca ttacttctta atagtgaaaa ggggttagat
     2401 agtaccaaat gggaaaatgt taagtaagta cttctctata ttcttcaggt aaactatcag
     2461 ctgatggttt atctatacaa aaaaggagtc gggatgaacg aacaatccca ggccaaatca
     2521 ccagaagcct tgcgcgcaat ggtcgccggg acactggcca attttcagca cccaaccctg
     2581 aagcataacc tcaccacgct gaaagcgttg caccatgtcg cctggatgga cgacacgttg
     2641 catgtggaac tggttatgcc gtttgtctgg catagcgcgt tcgaagagtt aaaagagcaa
     2701 tgtagcgccg aactgctgcg catcaccggg gcgaaagcta tcgactggaa actgtcgcat
     2761 aacatagcca ctctgaaacg cgtgaagaat cagccgggaa ttaacggtgt gaagaacatt
     2821 atcgcggtga gttccggtaa aggcggcgtg ggtaaatcgt ccacggcggt aaacctggcg
     2881 ctggcactgg cggcggaagg ggcgaaagtc ggcattctgg acgccgatat ctacggccca
     2941 tcaattccca ccatgctggg cgcagaaaat cagcgtccaa cttcgccgga cggtacccac
     3001 atggcaccga ttatgtctca tggcctggca accaattcaa ttggttatct ggtcactgac
     3061 gacaatgcga tggtgtggcg tggaccgatg gcgagcaagg cgttaatgca gatgttgcag
     3121 gaaaccttgt ggccggatct cgactatctg gtgcttgata tgccgccagg caccggtgac
     3181 attcaattga cgctggcgca aaacattcca gtaactggtg cagtcgtggt caccaccccg
     3241 caggacatcg cgttgatcga tgcgaaaaaa ggcattgtga tgttcgagaa agtcgaggtt
     3301 ccggtgctcg gtattgtcga gaacatgagt gtgcatattt gcagtaactg tggtcaccat
     3361 gaaccgattt tcggtaccgg tggtgcagag aaactggctg aaaaatacca tacacagctg
     3421 ctggggcaga tgccgttaca catctcctta cgtgaggatc tcgataaagg cacgccaacg
     3481 gttatcagcc gtccggagag cgaatttacc gctatctacc gccagctggc tgaccgcgtt
     3541 gcagctcagc tctactggca gggtgaagtc attcctggcg agatttcctt ccgcgcggtc
     3601 taacgcctat taaccattct ggttattctt catacaccgc agagatgtta tattgatgcg
     3661 gtgtatttgt ttttgccgca ttaatgaaca cttatttaat actgcgaata ttctgcggac
     3721 cttcgtccga aactctacaa agtcgagtga tcgtacaggc aatgcttcat tatcatcctt
     3781 cgtgctgtgt agtggatgta cagctatagt ttgttatgca ctcattctat ccaggcataa
     3841 cctttactac agggataacg ttcaatgaat aagtattggc tgtcaggaat tatttttctt
     3901 gcctatggat tggcttctcc cgcagtctct tcagagactg ccacattaac gattaatggc
     3961 aggatcagtc ccccaacatg cagtatggct atggttaata gtcaaccaca acagcattgt
     4021 ggtcagttaa cgtataatgt tgacacccgt catcaggttt cttcgcccgt caaaggtgtt
     4081 acgacagaag tggttgttgc aggtagcgat agcaaacgtc gaatcgtgtt aaatcgctac
     4141 gattaatttg gcaacctgat tttaattgca tttagaaaga cgaaatcatt tatataaatg
     4201 atttcgtctt ttttattgat atcaacaata ccatttacat aaatctacat taattaatag
     4261 atgtgataaa tatttaatat tcttaagtat ttgttgtatt tatattgcat tggttaattt
     4321 gttttaaatc aataaaacaa tatgtttaca ttgcggcagc ttttttgatg tgagaaattt
     4381 gtttccaaga gatacagact cttaacaacc aaaaataaat tttattaagg aacatattat
     4441 gaaacgttca attattgctg ccgctgtctt ttcttctttt tttatgagcg ctggagtatt
     4501 tgctgcagac gttgataccg gaacattaac cattaaagga aatattgcag aatctccgtg
     4561 taaattcgaa gcgggtggtg attcagtaag tattaatatg ccgactgtac caaccactgt
     4621 ctttgaaggt aaagctaaat attctaccta tgatgatgca gtcggtgtaa ccagcagcat
     4681 gttaaaaatt agctgcccga aagaagttgc tggtgtaaaa ctctcgttga tcacaaacga
     4741 caaaataacc ggtaacgata aggcgatagc cagtagcaac gataccgtag gtgataacag
     4801 cgatgtccta gatgtttctg caccttttaa cattgagagt tataaaacag cggaaggtca
     4861 atatgctatt ccgtttaaag caaaatacct gaaactgaca gataactcag tgcaatcagg
     4921 tgatgtgtta tcttctctgg ttatgcgtgt ggcgcaggat taataaataa aattgcaagt
     4981 ttctccaggt aataaacatg gctgctatcc ccatggcggc tttttaattt aagaggcatt
     5041 aagatgaaag gattattatc tttactcatt ttttctatgg tccttcctgc acatgccgga
     5101 attgttatct acgggacgcg cattatttac ccggcagaaa ataaagaagt tatggtgcag
     5161 ttgatgaacc agggaaatcg ttcttcgctg ctgcaggcgt ggattgatga tggcgatacg
     5221 tcgttaccac cagaaaaaat tcaggttcct ttcatgttaa cgccaccagt ggcaaaaata
     5281 ggggcaaatt ccgggcagca agtaaaaatc aaaattatgc cgaataaact gcccactaat
     5341 aaagaaagca ttttttatct gaatgttctg tatattccac caaatagtcc agagcaagaa
     5401 ggcaagaatg cactgaagtt tgcgatgcaa aacagaatta agttgtttta ccggccagcg
     5461 ggtattgctc cggtaaataa agcgaccttt aaaaagttac tggtaaatcg cagtggcaat
     5521 ggattggtga taaaaaatga ctcagctaat tgggtgacga tttcggatgt caaagctaat
     5581 aatgttaaag tcaattatga aactattatg attgccccct tagaaagtca gagtgttaat
     5641 atcaaaagta ataatgcaaa taactggtat ctgaccatta tcaatgacca cggcaactat
     5701 attagtgaca aaatttaatt gcaggagctg cctatgttga gaatgacccc acttgcatca
     5761 gcaatcgtag cgttattgct cggcattgaa gcttatgcag ctgaagaaac ctttgatacc
     5821 cattttatga taggtggaat gaaagaccag caggttgcaa atattcgtct tgatgataat
     5881 caacccttac cggggcagta tgacatcgat atttatgtca ataagcaatg gcgcgggaaa
     5941 tatgagatta ttgttaaaga caacccgcaa gaaacatgtt tatcaagaga agttatcaag
     6001 cggttaggca ttaatagcga taacttcgcc agcggtaagc aatgtttaac atttgagcaa
     6061 cttgttcagg gtgggagcta tagctgggat atcggggttt ttcgtctcga tttcagtgtc
     6121 ccgcaggctt gggtggaaga actggaaagt ggctatgttc caccggaaaa ctgggagcgg
     6181 ggtattaatg cgttttatac ttcttattat gtgagtcagt attacagcga ctataaagcg
     6241 tcgggtaaca acaagagtac atatgtacgt tttaacagcg ggttaaattt actggagtgg
     6301 caactgcatt ctgatgccag tttcagtaaa acaaataaca atccaggggt gtggaaaagc
     6361 aataccctgt atctggaacg tggatttgcc caatttctcg gcacgcttcg cgtgggtgat
     6421 atgtacacat caagcgatat ttttgattct gttcgcttca gcggtgtgcg gttgtttcgt
     6481 gatatgcaga tgttgcctaa ctcgaaacaa aattttacgc cacgggtgca ggggattgct
     6541 cagagtaacg cgctggtaac tattgaacag aatggttttg tggtttatca gaaagaggtt
     6601 cctcctggcc cgtttgcgat tacagatttg cagttggccg gtggtggagc agatcttgat
     6661 gttagcgtga aagaggcgga cggctcggta accacctatc tggtgcctta tgcagcggtg
     6721 ccaaatatgc tgcaacccgg cgtgtcgaaa tatgattttg cggcgggccg tagccatatt
     6781 gaaggggcga gcaagcaaag tgattttgtc caggcaggtt atcagtatgg ttttaataac
     6841 ttattgacgc tgtatggcgg cacgatggtt gctaataatt actatgcgtt taccctctga
     6901 acgggttgga acacgcgcat tggcgcaatt tccgtcgatg ccacgaagtc gcatagtaaa
     6961 caagacaacg gcgatgtgtt tgacgggcaa agttatcaaa ttgcctacaa caaatttgtg
     7021 agccaaacgt cgacgcgttt tggtctggcg gcctggcgtt attcgtcgcg tgattaccgg
     7081 acatttaacg atcacgtttg ggcaaacaat aaagataatt atcgccgtga tgaaaacgat
     7141 atctatgaca ttgccgatta ttaccagaac gattttggcc gtaaaaatag cttttctgcc
     7201 aatatgagcc agtcattgcc agaaggctgg ggttctgtgt ccttaagtac gttatggcga
     7261 gattactggg ggcgtagcgg cagcagtaag gattatcagt tgagttattc caataactgg
     7321 cggcggataa gctataccct cgcggcaagc caggcgtatg gcgagaatca tcatgaagag
     7381 aaacgtttta atatttttat atcaattccc tgtgattggg gtgatgacgt tacgacgcct
     7441 cgtcggcaaa tatatatgtc taactcaacg acgtttgatg atcagggttt tgcctcaaat
     7501 aatacgggat tatcaggaac agtagggagt cgggatcagt tcaattatgg tgtcaacctg
     7561 agtcatcaac atcagggaaa tgaaacgaca gctggggcga atttgacctg gaacgcgccg
     7621 gttgcgacag tgaatggcag ttatagtcag tcgagtactt atcgacagac tggagccagt
     7681 gtttcagggg gcattgtcgc ctggtcgggt ggcgttaatc tggcgaaccg tctttccgaa
     7741 acgtttgctg tgatgaatgc gccaggaatt aaagatgctt atgtcaatgg gcaaaaatat
     7801 cgcacaacaa accgtaatgg agtggtggta tacgacggaa tgacacctta tcgggaaaat
     7861 cacctgatgc tggatgtgtc gcaaagcgat agcgaagcag aattacgtgg caaccggaaa
     7921 attgccgccc cttatcgcgg cgcggttgta ctggttaatt ttgataccga tcagcgcaag
     7981 ccctggttta taaaagcgtt aagagcggat ggtcaaccat taacgtttgg ttatgaagtc
     8041 aatgatatcc atggtcataa tattggcgtt gtcggccagg gaagccagtt atttattcgc
     8101 accaatgaaa taccgccatc ggttaatgta gcaattgata agcaacaagg actttcatgc
     8161 acaatcacct tcggtaaaga gattgatgaa agtagaaatt atatttgcca gtaatagtca
     8221 ggtggttcta tggaaattcg cataatgcta tttatattaa tgatgatggt tatgcctgtg
     8281 agctatgcgg catgttatag tgagttatct gttcagcaca atttggttgt tcaggggaat
     8341 tttgcactta ctcaaacaca aatagcgaca tatgagcata attttaatga ttcgtcatgc
     8401 gtgagtacaa atactatcac ccctatgagc ccatcggata ttattgttgg actttataac
     8461 gataccatta aattaaattt acattttgaa tggaccaata aaaacaacat cacgttgtca
     8521 aataatcaga gcagtttcac cagtggttat tcggtaacgg tgacacctgc ggccagtaat
     8581 gcaaaagtga atatttctgc ggggggcggt ggttcagtga tgattaatgg tgttgcgaca
     8641 ttatccagtg cttcatcatc gacacgcggg agtgccgcag tacaatttct actgtgttta
     8701 ttaggtggca agtcatgggg tgcatgtgta aatagctaca gaaatgcatt ggcacaaaat
     8761 gcaggtgtct attccttcaa tctgacattg tcatacaacc cgataaccac aacctgcaaa
     8821 caggacgatt tattaattac tttagacagt attcccgttt cacaattacc agccacaggt
     8881 aacaaagcaa caataaatag taaaaaaagg gatattattc tgcgttgtaa aaatttatta
     8941 ggtcaacaaa atcaaacatc acggaaaatg caggtgtatt tatcaagttc tgacttgtta
     9001 accaacagca acacaatact gaaaggtgcg gaagataatg cgtaggattt attcttgaaa
     9061 gtaatggttc gccagtcaca cttttaaata tcactaacag cagtaaagga tacacaaatt
     9121 taaaagaaat tgcggcaaag tcaaaactta cagatacaac ggtttcaatt ccgataacag
     9181 ccagttacta cgtctacgat acaaacaaag ttaaatctgg cgcactggag gcaaccgcat
     9241 taatcaacgt gaaatacgac taataacaag caaaacgagc aagtggctaa taataaaaaa
     9301 taacaaggtg tctgcatttc cccctcatga tgagggggct ttttttagcg atgataaaaa
     9361 atctcaccgt cgtaggcttt aatgatttta ccgtcggtgt cgctgatcaa cacgtacgcg
     9421 ccacccatat aggtccagtg cgtcccggca tcaggcgcgg gcaggttacg taactgccac
     9481 tgcttaatgt tgtactcatc ggtgcggtac atctctggga cggtatcacc tggtttgaaa
     9541 tgcttaaagt cagcaatgaa actacttaac tcatacttag aaattcccga ggtattggtc
     9601 gaacccgcgg tggctggtgc ggcccaggcg gcactggtaa ccagcaaaag cacacccagc
     9661 aacatcttat ttttaatggt cataaactct ccactttggg gatctgtaat atcagtcgca
     9721 tatctttcct gtcatacaat ataactgaca agcgatattt tgttagtgat cccatgattt
     9781 cgtaaaaagt gtaaatgaaa ggatgcccta aaagcatcct tagcgagcag agagtcagcg
     9841 gaaatagctg atttaagaat catcctgggc tgaagataac atgcaagcct aaaggattga
     9901 gttatcgcat tatgcccatg aagccatgta cgcccatata cacaccgact aagccaatca
     9961 acagactgga aaaatagggg gcgcgtttag cgagagtgtt aaatccgctc cagcgttttg
    10021 cgacctgctg aacgctgatt gctgcgccaa cgcctacggt gacaagcgtt aacgccaggc
    10081 caatgctgaa actgacgacc agtgttgcgc ccagtgtcag ggctttcaac tgaatgcata
    10141 tcaacagcac ggtaattgct gccgggcagg ggataaagcc accggttaag ccaaataaca
    10201 aaatttgcca gttgatgacc tctctaccat caaagcgtcg tttaatgtca ttggcatggg
    10261 ctcgtgcatg ggcatcctga tactcgccat attcgtgatg gtgatgatgt ccttggtcgt
    10321 ggtgatgttc gtggtcttga tgatgttcgt gatcgtgatg atggtgatga tgctcataat
    10381 catgcccgtg catattctcc agccagttgc gttcgccgcg ctaggtacgc cagaacatcc
    10441 agaacgcggt gccaataatg atcactgcgg aaatcagctg gagccacggt tctgctgatt
    10501 gagcagtaaa gcgcttactg atcaccatcc cgccaaaggc aatcaaccag accaccgcgg
    10561 tatgcgaaat ggttgctgcc agtccgagca tcaccgcctg tttaatggtg cctttgatgg
    10621 cgatgataaa cgccgccatc atcgtttttg agtgccctgg ttccaggcca tgaagcgcac
    10681 caagtaagat ggcgctgggg atgaagaacc aggcgtttcc ttgctgaaga agagttgtaa
    10741 attcggtcat gataataatt cttagtatta attcggcaat ctgattctac tcccccccag
    10801 tacctgatac taccccccca gtagattaat agtgctatga tttttcatat tcttgttaac
    10861 caggtgttgc catgtctcat acaatccgtg ataaacagaa actgaaagcg cgtgccagta
    10921 agattcaggg ccaggtcgtg gcgctcaaga aaatgctcga cgagccgcac gaatgcgctg
    10981 cagttttaca acagattgct gctatccgtg gagcggtaaa cggtctgatg cgggaagtga
    11041 ttaaaggtca tctgacggaa cacatcgttc accaggggga tgagctaaaa cgtgaagaag
    11101 atctggatgt cgttctgaag gtgctggatt catatatcaa ataatttatt aacgcgattg
    11161 taaaactgcc gtttttcctc gtttacaacg ggggcgctgg acattaccat cctcctctgc
    11221 gatttatcat cgcaaccaaa cgactcgggg tgcccttctg cgtgaaggct gagaaatacc
    11281 cgtatcacct gatctggata atgccagcgt agggaagtca cggaccacca ggtcattgct
    11341 tcttcacgtt atggcaggag caaactatgc aagtcgacct gctgagttca gcgcaatctg
    11401 cgcacgcgtt acaccttttt caccaacatt cccctcttgt gcactgcatg accaatgatg
    11461 tggtgcaaac ctttaccgcc aataccttgc tggcgctcgg tgcatcgcca gcgatggtta
    11521 tcgaaaccga agaggccagt cagtttgcgg ctatcgccag tgccttgttg attaacgttg
    11581 gcacactgac gcagccacgc gcacaggcga tgcgtgctgc cgttgagcaa gcaaaaagct
    11641 ctcaaacacg ctggacgctt gatccagtag cggttggtgc gctcgattat cgccgccatt
    11701 tttgtcatga acttttatct tttaaaccgg cagcgatacg tggtaatgct tcggaaatca
    11761 tggcattagc tggcattgct aatggcggac ggggagtgga taccactgac gccgcagcta
    11821 acgcgatacc cgctgcacaa acactggcac gggaaactgg cgcaatcgtc gtggtcactg
    11881 gcgaggtgga ttatgttacc gatggacatc gtatcattgg tattcacggt ggcgatccgt
    11941 taatgaccaa agtggtagga actggctgtg cattatcggc ggttgtcgct gcctgctgtg
    12001 cgttaccagg cgatacgctg gaaaatgtcg catctgcctg tcactggatg aaacaagccg
    12061 gagaacgcgc agtcgccaga agcgaggggc caggcagttt tgttccacat ttccttgatg
    12121 cgctctggca attgacgcag gaggtgcagg catgaaacga attaacgctc tgacgattgc
    12181 cggtactgat ccgagtggtg gtgcggggat tcaggccgat cttaaaacct tctcggcact
    12241 tggcgcttat ggttgctcag ttattactgc actggtggcg caaaataccc gtggcgtaca
    12301 gtcggtgtat cgcattgagc ctgattttgt cgccgcccag ctcgattcgg tgttcagcga
    12361 tgtgcgaatc gataccacta aaatcggtat gttggcggaa accgatattg ttgaagcggt
    12421 ggcagaacgg ttgcaacgtt atcagatcca aaacgtggta ctcgacaccg ttatgctggc
    12481 aaaaagcggc gacccgctgc tttcaccttc ggcggttgct acgctgcgca gtcgattatt
    12541 gccacaggtt tcattaataa cgccaaactt gcccgaagct gccgccttgc tcgacgcgcc
    12601 acacgcgcgc accgaacagg aaatgctgga acaagggcga tcgctgttgg cgatgggctg
    12661 tggcgcagtg ctaatgaaag gtggtcatct ggatgatgag caaagcccgg actggctgtt
    12721 tacccgcgag ggtgaacaac ggtttaccgc accgcgcatt atgaccaaaa acacccacgg
    12781 cactggttgt acactctctg cggcgttggc tgcactacgc ccgcgccata caaactgggc
    12841 tgacaccgta caggaggcaa aaatctggct ttcatcggcg ttagcccagg ccgacacgct
    12901 ggaagttggt cacggtattg gtccggttca ccacttccac gcctggtggt gagcaccgta
    12961 ctatagctgc tttggttatt gtcagccttt ggcactgaca ttcagggatg aaaccaatgc
    13021 agctaagaat taccagtcga aaaaaactca ccgctctatt atgcgctctt gggctaattt
    13081 ccatcgttgc aatttatccg cgtcagacgg tgaatttttt ctattcgaca gcagttcaga
    13141 ttacagacta catccacttc tacggttatc gcccggttaa atctttcgct attcgcattc
    13201 ctgccagtta caccattcac ggattagatg tttcgcgctg gcaggagcgt atcgactggc
    13261 agcgggtggc aaaaatgcgc gacaacgata tccgcttaca gtttgctttt attaaggcta
    13321 cggaaggcga aaagctggtg gacccctatt tttcgcgtaa ctggcaatta agccgcgaaa
    13381 atggcctgct gcgcggggcg tatcattatt tctccccgtc ggtatccgct tcagttcagg
    13441 cgcgattatt tctgcaaacg gtggatttct cacaaggcga tttccctgcc gtgctggacg
    13501 tagacgaacg gggaaaatta tcggcaaaag aattacgcaa gcgggtaagt cagtggctaa
    13561 aaatggtcga aaaacgcacg ggaaaaaagc cgattattta ctcaggagcc gttttttatc
    13621 acaccaatct ggcgggctat ttcaatgaat atccgtggtg ggtggctcac tattatcaac
    13681 gtcgtccgga caatgacggc atggcctggc gcttctggca gcattccgac cgtggacagg
    13741 tagatggcat caatggtccg gtggatttta atgtgtttaa tggcacgggg atgagttgca
    13801 ggcattcgtt gatgggatta aagaaacgcc ttaaataaga agcagtgata tactcccacc
    13861 agactccctg gcaggaaaaa gacaaatgga acaagcgcat acccagctga ttgcccaact
    13921 gaacgagcgt attttagcgg cggataacac gccgctttat attaagtttg ccgaaacggt
    13981 aaaaaatgcc gtgcgcagcg gggtgctgga gcatggcaat attttgcccg gtgagcgtga
    14041 tttaagtcag ttaaccggcg tttcgcgcat tacggtgcgc aaggcgatgc aggcgctgga
    14101 agaagagggc gtggtgacgc gttcgcgcgg ttacggcacg cagatcaaca acatcttcga
    14161 atactcgttg aaagaagcga gaggtttttc tcagcaggtg gtattgtgcg ggaaaaagcc
    14221 cgatacgcta tgggttaata agcgtgtcgt gaaatgtccc gaagaagttg cgcagcagct
    14281 ggcggtcgaa gcgggaagtg atgtcttttt gcttaagcgt attcgctatg tcgatgaaga
    14341 agcggtatcg attgaggaat cgtgggttcc ggcgcattta attcatgatg ttgatgccat
    14401 cggaatttct ctttatgact atttccgcag ccagcacatt tacccacaac gtacgcgttc
    14461 ccgcgttagc gcccggatgc cggatgccga gtttcagtca catattcagt tagatagcaa
    14521 aataccggtg ctggtgatca agcaagtggc gcttgaccaa cagcaacggc ctattgagta
    14581 cagcattagc cactgtcgca gcgatctata cgtttttgtg tgcgaggagt agttcctcgc
    14641 gcgttggcgc acaatcaccg ccccgatgcc cgacaaccca cgacgccact gcattgccca
    14701 gtaatacggc atccgccagt ggcagaccag aggccagccc ggcaagtacg ccacctgcat
    14761 gactgtcgcc cgccccaatg gtgtctacaa cttgcgttgg aaatgccgga atgcagccag
    14821 aagcgtcgtt gctgaaatac catgcgcctt ctttatcgag acgaacgatc aacggcgcgg
    14881 caaatttctc ctgccattgc ttgccaagtg ttgttatctc tgcggataaa gcaaaacgtt
    14941 cggcggcaat ctcagcctct tgacgattga gtgacactaa aggtcgacag gccatgatcc
    15001 gcgccagtaa tgcgtccggg atatcgccaa tacgtgggca aaaatcgata aacggcgtca
    15061 cgtcttgcag cttttccagc cattccacta acaattcgcc gcagggcgag gccagttgat
    15121 aaccggaaaa atagagcagg ttgccaggcg caacggttaa tcgtgccaac cattggcgat
    15181 tccattgatt ttccacaccg ctgaatgaca taaaagtgcg ttcgccatcc ggctcaacca
    15241 gtgccagaca ccagccgtta tcgctttcgg cgttatcgat caggctgatt aacccctctt
    15301 tcgccatccg gttgcgaata atctccgccc acacgccctg accgagcggc aaggcattac
    15361 ccgcttcgat gccgaggcgc tttaacgcca cggcaatatt cagtgcgcag ccgccaacgt
    15421 taacgctctg ctgtttcagt tcgatatcgc acccacgcca ggggagggca taagcgtcgg
    15481 cgatcacgtc aatgaccgcc gcgccgacga ccatcaccgg ctgacgcgtg gttaattcag
    15541 gcagcagtgt gtgtaatcga gcgccgctca taccgcctcc cgttgttgac gatatttcgc
    15601 cagcgctgtg gcatagcggt tgaaatcaag ctgatttacc gcatccagct ccgcctttaa
    15661 cgcaggatcg acagcgttaa tgccatgcaa cgcgccgcaa atcgccgtcg ccatagcacc
    15721 tatggtgtct gtgtcgccgc caagattggc gcacaggacg gcgcagcggt ttgggtcggt
    15781 ttgtgccagt tcaactagcg caatggcgca cggaacggac tcaatagtgc tggtgcctgc
    15841 gccaacgacc tggtaaagct gttcgctggc ggattcggtg ccgtcggcat tacgcacaat
    15901 tttcagcgca atctccagac gtgccgccag tgaggcgctg aacgtggtgg tgcgtttttg
    15961 ctgtgcatgt cgcgcaattg aaggcaatga atcaacaatc gctgaccagc gttctccgtc
    16021 aatggcacga gaaatcgccc atgcgatgac taccgcgcct gcaaccgcca gatcggattt
    16081 atgggtcggg ctggacgcca gcgccacatc atcaataaag gaatcaacat cacgcgctgg
    16141 aagcaaacaa cctaatggcg agacgcgcat cgctgcgccg ttggttacgc cgttgttttc
    16201 cagttcagca acgggtttac cgtcgcgaat ggcgttaagc gcaatcttcg aggtcggacc
    16261 tagtacgttt ttgttaaagg cgtcgaaacg caacgcccag tcgagaatat tacgcccaat
    16321 cagattcgga tcgatcttgc cttcacattc cagtaacgca tccgccagac acaggccatc
    16381 gaggtatcgt cggtgaattc ggcgcggtta aaataacagg ccgcgttatt ctcctttggt
    16441 ccaggcagaa aacggtcaat ccagccaaag tgtgctttaa cgcggctgcg tggccaaagc
    16501 tcggagggca tccccatcgc atcccctaac gcctgcccat aaagagcacc gagaatacgt
    16561 tctgttttca tttaacttcc ccttgtgtca acgcaatatc tcgatcatcg accttgatag
    16621 ccgtaatttc gttgtcggat tcgcggaaaa aaatcatgaa cagcacggcg ataatggcaa
    16681 tcatcaccgc gccgaaagtc cacatcccgg accagttgaa agtcagtccg tttaccggtt
    16741 cctgataagc gaacatcttt tccatcatca caccgccaag acgatagccg agcaaactgc
    16801 cgaagccctg gcagcagagc gtgatcagcc cctgcgcagc ggtacgcata tgcacggggg
    16861 cttttttatc gacatagatg taagcggtaa cgtagtaaaa atcgtaactt acgccgtgaa
    16921 gcaaaatacc gaggaacagt aacgcgtagg tgaaatattc atccgcacta ccgtaaataa
    16981 agaagccata gcagatcgca gcggtgacca gaccaagcaa taataccttt ttgataccaa
    17041 agcgtttagt gaaaaacggc aatgccagca taaagaagat ttcagagaac tggccgagcg
    17101 tcatccagcc ggtggcgttt ttcatgccaa cttcggtcag ataaccgttg gcaaagatgt
    17161 aatagaacgc cagtggcatc gcaaacagga atgaacagaa gaaaaagacg aggaagtttt
    17221 tatcgcgcag caggatcagc gcatccaggc cgagcatgac tttaatgtcc attttgccgg
    17281 tgctttttgg cggcgtgtcg ggcaggaaaa acgcaaacac accgagcaga gcagaacttc
    17341 cggcggtaat cagcagcggg atgttagtcg gtgagatatc ggcatacccc agcatttgcg
    17401 gcaagaaacc acatgccaga ccggaggcaa tccagccgat agtgcccatc acacgaatgc
    17461 gtgggaaatc acgctccaca tccggcacgt tggcaaaagc gatgctgtta gtcagcgcaa
    17521 tggtcggcat ataggttagc gagtaggcca gcagtaacgg aaagaacccg gcaaaagtgg
    17581 tctgttgcgc agcgaaatac atcagcacag caccagcgaa catcaatacc gccagcactt
    17641 tctgcgccga gaaaaagcgg tcagtgatgg agccaaccag aattggcgac aggatcgccg
    17701 caatggctgt gcaggcatac gaccagccaa tttctccggc actaaaaccg cttttactta
    17761 accacaacca caatggcaca aaccacgcgc cccagataaa ccattcaaca aacatcatga
    17821 acgacagctt tgctgttgtt ttcatctttt aattccttca tgacaggtaa tgtaaggtac
    17881 gcgatgacaa taccacataa taatgccttt tgaatacctt tgcggtgaaa acttgaccgc
    17941 gctaacattt tttctgatga atcgagccaa cagaaaacgc tgaaaaaaca tccaaaagat
    18001 ggaaaaactc gcccgtaacg actaggatta tgattgcccg caaaaggcgg gccaggacaa
    18061 tccatagccg atatccaatc ggaatttacg ggagcatagt aatgacagat attgcgcagt
    18121 tgcttggcaa agacgccgac aaccttttac agcaccgttg tatgactatt ccttctgacc
    18181 agctttatct ccccggacat gactacgtag accgcgtgat gattgacaat aatcgcccgc
    18241 cagcggtgtt acgtaatatg cagacgttgt acaacaccgg gcgtctggct ggcacaggat
    18301 atctttctat tctgccggtt gaccagggcg ttgagcactc tgccggagct tcatttgctg
    18361 ctaacccgct ctactttgac ccgaaaaaca ttgttgaact ggcgatcgaa gcgggctgta
    18421 actgtgtggc atcaacttac ggcgtgttgg tgtcggtatc gcggcgctat gcgcatcgca
    18481 ttccatttct cgtcaaactt aatcacaacg agacgctaag ttacccgaat acctacgatc
    18541 aaacgctgta tgccagcgtg gagcaggcct tcaacatggg cgcggtggcg gttggtgcga
    18601 ctatctattt tggctcggaa gagtcacgtc gccagattga agaaatttct gcggcctttg
    18661 aacgcgcgca cgagctgggc atggtgacag tgctgtgggc ctatttgcga aactccgcct
    18721 ttaagaaaga tggcgttgat taccatgttt ccgccgacct gactggtcag gcgaaccatc
    18781 tggcggcaac cataggtgca gatatcgtca aacaaaaaat ggcggaaaat aacggcggct
    18841 ataaagcgat taattacggt tataccgacg atcgcgtgta cagcaagtta accagcgaaa
    18901 acccgattga tctggtgcgt tatcagttag ctaactgcta tatgggccgg gctgggttga
    18961 taaactccgg cggtgctgca ggcggtgaaa ctgacctcag cgatgcagtg cgtactgcgg
    19021 ttatcaacaa acgcgcaggc ggaatggggc tgattcttgg acgtaaggcg ttcaagaaat
    19081 cgatggctga cggcgtgaaa ctgattaatg ccgtgcagga cgtttatctc gatagcaaaa
    19141 ttactattgc ctgatacgcc tcatacgaac agcaccaccc cgtaggacgg ataagacgtt
    19201 tacactgcat ccggcaacct atgcctgatg cgacgcggaa gcgtcttatc atgcctacat
    19261 agcactgcca cgtatgttta caccgcatcc ggcataaaaa cacgcgcact ttgctacggc
    19321 ttccctatcg ggaggccgtt tttttgcctt tcactcctcg aacaattttc atattgttgt
    19381 ttttgtgatc attatctcga catttaaaag caaataattt cattatattt tgaaatcgaa
    19441 agcaaacgac aagatatgaa aatgtacgtg gtatcgacaa agcagatgct gaacaacgca
    19501 cagcgcggcg gttatgcggt tccggcattc aatattcaca atctcgaaac gatgcaagtg
    19561 gtggtggaaa ccgctgccaa cctgcatgcg ccggtcatca tcgccggaac gcctggcaca
    19621 tttactcatg ctggtacaga aaatctgttg gcgctggtca gcgcgatggc gaagcaatat
    19681 caccatccac tggcaattca tctcgaccat cacacgaaat ttgacgatat cgctcagaag
    19741 gttcgttctg gcgtgcgctc agtcatgatt gacgcctcgc atttgccttt tgcgcaaaat
    19801 atttcacggg tcaaagaggt ggtggatttt tgccatcgct ttgatgtcag cgtcgaggcg
    19861 gagctggggc aacttggcgg ccaggaagat gatgtgcaag tcaatgaagt cgatgcgttg
    19921 tacaccaacc ccgcacaggc gcgtgaattt gccgaggcaa ccgggattga ttccctggcg
    19981 gtcgccatcg gcacggctca cggtatgtat gccagcgcac cggtgcttga tttttctcga
    20041 ctggagaaca ttcgccagtg ggtgaactta ccgctggtgc tgcatggcgc gtcaggatta
    20101 tcgactaagg atattcagca aaccatcaaa ctggggatat gcaaaatcaa cgttgcaacg
    20161 gagctgaaaa atgccttctt gcagtcgtta aaaaattacc tgaccgagca ccccgaagcg
    20221 accgatcccc gggattattt gcagtcggct aaatccgcaa tgcgcgatgt ggtgagcaaa
    20281 gtgattgccg attgtggctg cgagggcagg gcataacgca cctgccattt aacaaggaaa
    20341 aaacatgaaa acgttaattg cccggcataa agctggtgaa catatcggca tatgttcagt
    20401 ctgttctgcc catccgttgg ttatcgaagc ggcgctggca tttgatcgca acagcacgcg
    20461 caaagtgctg attgaagcaa cgtcaaacca ggtcaatcaa tttggcggtt ataccggaat
    20521 gacaccggca gactttcgcg aatttgtttt tacgattgcc gataaagttg ggtttgcacg
    20581 cgaacgcatt attctcggcg gcgatcatct gggaccaaac tgctggcagc aagaaaatgc
    20641 ggatgcggcg atggaaaaat ccgtcgagct ggtaaaagca tatgttcgtg ctggcttcag
    20701 taaaattcat cttgatgcgt caatgtcctg cgcgggggat accataccgt tagcaccaga
    20761 aacggttgcg gaacgagctg ctgtgctttg ctttgctgcg gaaagtgtgg cgacagattg
    20821 ccagcgtgag caactgagct atgtcattgg cactgaagtt ccggttccgg gcggtgaggc
    20881 cagcgccatt cagtcagtac acatcaccca tgttgaagat gccgccaata ctttacgtac
    20941 gcatcaaaag gcctttattg cccgtgggct gacagaggcg ttaacacgtg tgattgccat
    21001 cgtggtgcag ccgggtgtgg aatttgatca cagcaatatt atccattatc agccgcagga
    21061 agcgcaggcg ctggcgcaat ggatagaaaa cacccgaatg gtttatgaag cacattctac
    21121 cgattaccag acccggacgg cttattggga attagtccgc gatcactttg caatattgaa
    21181 agtcggtccc gcattaacct ttgctttacg cgaggcgata tttgcactgg cacaaattga
    21241 gcaggaactt atcgcccccg aaaatcgcag cggttgcctg gcggtaattg aagaagtgat
    21301 gctcgacgaa ccgcaatact ggaaaaaata ttatcgcacg ggttttaacg attcattact
    21361 ggatattcgt tacagcctgt cggatcgtat tcgttattac tggccgcata gtcggattaa
    21421 aaatagcgtt gaaacgatga tggtgaatct gcaaggcgtg gacatcccac tgggcatgat
    21481 tagtcagtat cttcccaaac aatttgaacg cattcagtcc ggggaattat cagcaatgcc
    21541 gcatcagctt attatgaata aaatttatga tgttttgcgc gcctatcgct acggctgtgc
    21601 ggaataagga cggtatatga ctaacctgtt tgttcgtagc ggaatttctt ttgtcgatcg
    21661 tagcgaagtt ttaacctata tcggtaatga gatgctcgcg aaaggtgtgg ttcatgatac
    21721 ctggccacag gcattaattg ccagagaagc agaattccct accgggataa tgcttgagca
    21781 gcacgccatt gcaataccgc attgtgaggc gattcatgct aagtcgtcag ccatttatct
    21841 gttaaggcca acaaataaag ttcattttca gcaagcggat gatgataacg acgtggcggt
    21901 atcgttggtt attgcgttga ttgtggaaaa tccgcagcag caattgaaac ttttacgctg
    21961 tttatttggc aagttacaac agcccgatat cgtcgagaca ctaattactc ttcctgaaac
    22021 tcagttaaag gaatacttca caaagtatgt tttagattca gacgaataaa tccctctgta
    22081 acaataaaaa gtactattta tgaaacgcaa gattattgtc gcttgcggag gcgcggttgc
    22141 gacctctacg atggcggcgg aagaaattaa agagttgtgt cagagtcata atattcctgt
    22201 tgaattaatc cagtgtcggg ttaatgaaat agaaacctat atggatggtg tgcatttgat
    22261 atgtaccact gccagggtgg atcgtagttt tggcgatatt ccgttagttc acggcatgcc
    22321 ttttgtttct ggtgtcggta tcgaagcatt acaaaataaa attctgacta tcttacaggg
    22381 gtgacctatg ttttcagaag tcatgcgtta tattctcgac ctcggcccta cagtgatgct
    22441 gccggttgtc atcattattt tttctaaaat attaggcatg aaggcaggcg attgctttaa
    22501 agcgggtctg catatcggga ttggctttgt tggcattggc cttgtgattg gcttaatgct
    22561 ggattccatt ggtccggcgg cgaaagcgat ggcggaaaat ttcgacctga atctgcatgt
    22621 ggtcgatgtc ggctggccgg gctcttcacc aatgacctgg gcgtcgcaaa ttgcgctggt
    22681 ggcgattccg attgcgatat tggttaacgt ggcgatgtta ctgacccgta tgacgcgggt
    22741 ggtaaatgtt gatatctgga atatctggca tatgaccttc accggcgcgt tgctgcacct
    22801 ggcaaccggt tcatggatga tagggatggc gggtgtggta attcacgcgg cgtttgttta
    22861 taagctctgc gactggtttg cccgcgatac ccgaaatttc tttgagctgg aaggtattgc
    22921 tattccgcac ggtacgtcgg cgtatatggg gccgattgcg gtgctggtcg atgctatcat
    22981 cgagaaaatc ccaggcgtta accgaattaa atttagcgcc gacgatattc agcgcaaatt
    23041 tggccctttt ggcgaacctg tcaccgtggg ttttgtgatg gggctgatta tcggcatcct
    23101 cgcgggttac gatgtcaaag gtgtattgca gctggcggta aaaacggcgg cagttatgct
    23161 gctgatgcca cggatgatta aacccatcat ggatggttta acgcccatcg ctaagcaggc
    23221 ccgtagtcgt ttacaggcga agttcggcgg tcaggagttc ctgattggcc tggatcccgc
    23281 attactgctg gggcatacgg cggtggtatc ggcaagcctg atttttattc cgctcaccat
    23341 tttaattgct gtttgtgttc cgggtaatca ggtactgccg tttggcgatc ttgccactat
    23401 cggcttcttc gtggcgatgg cggtcgcggt gcatcgtgga aatctgttcc gcaccttaat
    23461 ctcgggtgtc atcattatga gcatcaccct gtggatcgcg acgcaaacta ttggtttgca
    23521 cacccaaccg gcggctaatg ctggggcgtt aaaagccggg ggtatggtgg cttcaatgga
    23581 tcagggcggt tctcccatta cctggttact gattcaggtt ttctccccgc aaaatattcc
    23641 cggtttcatt attatcggcg caatttatct gaccggtatt ttcatgacct ggcgtagagc
    23701 gcgtggtttt actaaacaag agaaagccgt tctcgcagaa taatttttac ctgaggggga
    23761 ggttatcccc cttaacaatc aggagttttt atgaaatcag tagtgaatga tactgatggt
    23821 atcgtgcgcg ttgcaaaaag cgtcattcct gaaattaaac atcaggatga ggtgcgggta
    23881 aaaattgcca gctcgggctt atgtggttcc gatttaccca ggatatttaa aaatggtgca
    23941 cattattatc caataacgtt aggccatgaa tttagcggct atattgatgc ggtgggatcc
    24001 ggtgttgatg atttacaccc tggcgatgcg gttgcctgtg tgccgttatt accctgtttt
    24061 acttgtccag agtgtttgaa agggttttat tcccagtgcg caaaatatga ttttattggc
    24121 tcgcggcgtg atggtggatt tgctgaatat attgtcgtta agcgaaaaaa tgtctttgct
    24181 ctacccacgg atatgcctat tgaggatggg gcttttattg agccgattac cgttggcctg
    24241 catgcttttc atttagcgca aggttgtgag aataaaaacg ttattattat tggtgccgga
    24301 accattggcc tgctggctat tcagtgcgct gtcgcgctgg gagcaaagag tgtgacggcg
    24361 atcgacatta gctcagaaaa actggcactg gcaaaatctt tcggtgcgat gcaaacattt
    24421 aacagtagcg aaatgagcgc gccgcaaatg cagagcgttt tacgcgaact gcgctttaat
    24481 cagcttatcc tcgagacggc tggcgtaccg caaactgtcg aactggcggt agagattgcc
    24541 ggtcctcatg cccaactggc gctggtgggc acgttgcatc aggatctgca tttaacatcg
    24601 gcaacgtttg gcaaaatatt gcgtaaagag ctgacggtta tcggcagttg gatgaactat
    24661 tccagccctt ggccggggca ggagtgggaa acggcgagcc ggttgctgac agaacgtaag
    24721 ttaagcctgg agccattaat cgctcaccgt ggaagctttg aaagcttcac ccaggcggtg
    24781 cgtgacatcg ctcgtaatgc catgccgggc aaagtgttgc tcattccatg aaaccgcggg
    24841 ccagcgtgat gctggcccgg tattgtgcaa aacagatcat tcaccaatgg tcccccttcg
    24901 tttacactag ccacaattga atatgggtaa atgacgatga attcattcga gcgaaggaat
    24961 aagatcatcc aattagtgaa tgaacaggga accgtgcttg ttcaggatct ggcgggagta
    25021 tttgctgcct cggaagcgac aatccgtgcc gatttgcgct ttctcgaaca aaaaggcgtg
    25081 gttacgcgct ttcatggcgg tgcggcgaaa ataatgtccg gtaatagtga aaccgagacc
    25141 caggaagtcg ggtttaaaga gcgatttcag ctcgccagcg cgccaaaaaa cagaatagcg
    25201 caggcggcag tcaaaatgat ccatgaaggg atgaccgtta ttctcgacag cggaagtacc
    25261 acaatgctta tcgccgaagg attaatgacc gctaaaaata tcacggtgat taccaacagt
    25321 ctcccggcag cgtttgccct ttccgaaaat aaagacatta ctctggtcgt ctgtggtggc
    25381 acggtccgcc ataaaacgcg ctcgatgcat ggttccattg ccgagcgttc attgcaggat
    25441 attaatgccg atttaatgtt tgtcggtgct gatggtattg atgcggttaa tggtattacg
    25501 acctttaatg aaggttattc gattagcggg gcgatggtca ccgccgcaaa taaagtgatt
    25561 gctgttctcg attcatcgaa atttaatcgt cgcggtttta atcaggtact gccaatcgaa
    25621 aaaattgaca ttattgttac cgatgatgct gtgtcagagg tggataaatt ggcattgcag
    25681 aagacacggg taaaattaat tacggtgtag tattgttctg tatgccggat aaggtgttta
    25741 caccgcatcc ggcattacgg attctccctg gttcaagaga gaatagtttg attaacgcaa
    25801 taatggacaa tctggtggta atcgacaacg caacgccgcc ggaagtattt caatatggaa
    25861 gttttgccca ctcaacggtt cgccatcaag attaaaagtg atttcgtgtg gggcttgtat
    25921 atcaaaccac gacgaagcgc cttcgataat attcgggtta tcttcgtcag attttaaggt
    25981 tgatacgaga gccggaagaa tttcatcgcc ggtaaaaatg cgcagttgca gcaagccatc
    26041 gttaattaac gcgttcgggc acaattgttg accgccaccg gcctgacgcc cgttaccaat
    26101 accaatgacc agggcgtcac cttgccagtg aaagttttca ccgcggattt cacaacggtc
    26161 cggttgcaga gtatccatgc gcattaagcc atgaatgatg taagagacgc cacccagcgc
    26221 ggcttttaat ttttccggcg tttctgtggt aatacgcgtc ccaaatccgc ctgtcgccat
    26281 attaataaaa caggtttgtt tgttgacctg cgccatatct atcgcaatgg cattaccggc
    26341 aattgccagt ttcagcgcct tatccagtgc ctcaggaatc cctacactgg tggcaaaatc
    26401 attggcggtt cctaatggca aaattcccag cgcgggtatg tcatccccct cacactgaat
    26461 caacgccgta gaaacttcat taatggtgcc atcgccacca ccggcaatca ccgttgcgac
    26521 gcccaacttc cgggcctcct ctacaaatcg tgcggcatca cctttctccc aggtgacccg
    26581 cacatggatc gtcattcctt cctcacgcaa cagcataatt gcttcgcgca agggtagatt
    26641 gtcagtactt ttaccattaa gaatcagtaa gctggcggga aattctgcca tgatcgtatg
    26701 tgcctttatg attggtctgt agatagtgta gagcagaaaa caaaaggtgg agtcagaaca
    26761 ggatgaaagt cggaggataa aaagtcagtt caagtatgga ggattcagtg gttaccacca
    26821 ggagctttca atggggacag cgaatactaa cggctgccac cgataaattt caaaaaagag
    26881 catataccta atattcaact aaacagtggc atcttcaata taatatatta aagcccccat
    26941 ggagttaccc tgaagggcct caatgtccgt aattcctact tatgtaggaa atgttgtaca
    27001 gagcatttat tataatccta ttcaattata ataatcatgt cattattata tttaaacact
    27061 agagagtgtc gttggtattt aatgggggaa ggtgagatga aaaagatagc tgctatatca
    27121 ttaattagta tttttcttat atctggttgt gctgtgcata atgatgaaac aagtatcggt
    27181 aaatttggtc ttgcatataa aagtaatatt cagcgtaaac tcgataacca atactacacc
    27241 gaagccgaag cttctttagc caggggcaga atatctggtg cagaaaatat agtaaaaaat
    27301 gatgcagccc atttctgtgt tactcagggc aaaaaaatgc agatagttga cctgaagaca
    27361 gaaggtgcag gattacatgg cgtcgctcgt ctgacattca aatgtggaga gtgagaatat
    27421 ttttttgtaa gcgtcaaaca tgcgcgttct ggctgtgcgt agccggaacc tgggcgagca
    27481 cgataccgtt acgtgaaagg cattgtgcta tgaaggggga ttctatcgat gtggtcaatg
    27541 gaagacggtt atcagggatc aatccttctt tacagcttat gtacaaaaaa taagcccgtg
    27601 taagggagat tacacaggct aaggaggtgg ttcctggtac agctagcatt ttatggatta
    27661 tgtttttcag cgaaacggat gataacctta ataaatgcag ctgtatgtga tcggtttcta
    27721 agaattttcc atccgggaaa aataatcgaa attaatcact taccgtgggg gttacgcgtg
    27781 gtttccccgg agaaattacg cattagcagc gcgtaattta gctcaagatc ctgcgggacc
    27841 gggagccaca cagtataacc atcgcctggt gcgaccggca tagcttcgcc tttggcgttt
    27901 tccatgtgct caagggtaaa gttaatgttg ccttgcggcg tcatcagctc aagactgtcg
    27961 ccaacggaga atttattttt caccgctacc gctgcgaggt cccccttgcg ctcaccggta
    28021 aactcaccaa caaactgctg gcggtcagaa actgaataac cgtattcgta gttctgataa
    28081 tcgtcgtgag tatgacgacg caggaaacct tcggtatagc cacgatgcgc cagaccttcc
    28141 agagtttcca gcaggctggt atcgaacggt tttcccgcag cggcgtcatc gatagctttg
    28201 cggtaaacct gtgcggtgcg tgcgcaatag tagaaagatt tggtacgacc ttcgattttc
    28261 agcgaatgca cgcccatttt ggtcaggcgt tctacatggg cgatggcgcg cagatctttc
    28321 gagttcatga tgtaagtgcc gtgctcatct tcaaacgcgg tcatatactc gcccggacgc
    28381 tgggcttctt cgatcataaa cactttgtcg gttggcgcgc cgatacccag cgtcggctca
    28441 acattttgca ccggaatcgg ctcgtacttg tgtacgatgt tgccaacgtc atcttctttc
    28501 ccttcctgga cgttgtactc ccagcggcag gcgttggtgc aggtgccctg gttcgggtcg
    28561 cgcttgttga tatagccaga gagcaggcag cgaccggagt aggccatgca cagtgcgccg
    28621 tgaacgaaga tctcgatctc catatccggc acctgattat ggatctcttc aatctcttcc
    28681 agcgacagct cgcgagagag gatcacgcgg gtcagtccca tttgctgcca gaatttcacc
    28741 gtcgcccagt tcacggcgtt agcctgcacc gagaggtgga tcggcatttc agggaagtgc
    28801 tcacgcacca gcataatcag ccctggatcg gacataatca gcgcatccgg ccccatttcc
    28861 accaccggtt tcaggtcacg gataaaggtt ttcagcttgg cgttgtgcgg tgcaatgttg
    28921 accacgacat aaaacttttt ccccagcgcg tgggcttcat tgatgccgag ctgaagattt
    28981 tcgtggttga attcgttgtt gcgcacacgt agggagtaac gcggctggcc cgcataaaca
    29041 gcatctgcgc cataagcgaa agcgtaacgc atatttttca gcgttcccgc cggggaaagg
    29101 agttccggtt taaacataat tctctcgttc tgatgacagg tcagatccgc ttcacctgat
    29161 gaagcggtta ggggaggccc ccactttaag ggcgggcatt gtagcgctgt aggggggatc
    29221 tggtaaagcg ccggacgcca acaacccacg cccggcatcg taattacgta ttgtctttca
    29281 ccgtctggct tgtgccattg gctttaacgg aggcaattcc ggtttcacga gattgcgcgg
    29341 tggcgtacat ctggctggag ccgataattt gatgattagc ggctttcaga ttgaaataga
    29401 atttgccgtt gcttgccgtt ttttctcata gcgctcttct tgcgggctgt tgctacgcac
    29461 cgacgcgatg cccttttccg cagaggcttt tgaggtatac agctcgctgg tgaggatagt
    29521 ctcaccattg cccgctttta gcacaaaccg gaactgatta tcactgctct tactgagttc
    29581 aaaccaacca gccataatga ctccttgatt gaaattgctt atcgaaaaca gcatct
//
LOCUS       DAJTQY010000046        29257 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.046, whole genome shotgun sequence.
ACCESSION   DAJTQY010000046 DAJTQY010000000
VERSION     DAJTQY010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29257
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.046"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            <1..322
                     /locus_tag="OO349_002108"
     CDS             <1..322
                     /locus_tag="OO349_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCR5867171.1"
                     /translation="DITMLGGHSSHSYQNGTNMYFVYDYNVVDCKPEEEIDKYHNPLN
                     KIICEETIRLGGSMVHHHGIGKHRVHWSKLEHGSAWALLEGLKKQFDPNGIMNTGTIY
                     PIEK"
     gene            344..1753
                     /locus_tag="OO349_002109"
     CDS             344..1753
                     /locus_tag="OO349_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5867172.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     ATLTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLCQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            1731..2510
                     /locus_tag="OO349_002110"
     CDS             1731..2510
                     /locus_tag="OO349_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HCR5867173.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGNSGPDVSLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQRQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            2507..3367
                     /locus_tag="OO349_002111"
     CDS             2507..3367
                     /locus_tag="OO349_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HCR5867174.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDAMVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSMRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     SRDPLAEARRVLVVGQGGEADNQEIVMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFMVAINHDASAAVFSQADIGVVDDWKVVLEA
                     LVTNIHAEYQ"
     gene            complement(3515..4090)
                     /locus_tag="OO349_002112"
                     /pseudo
     CDS             complement(3515..4090)
                     /locus_tag="OO349_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
     gene            complement(4107..4367)
                     /locus_tag="OO349_002113"
     CDS             complement(4107..4367)
                     /locus_tag="OO349_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCR5867175.1"
                     /translation="MSVARNLWRVADAPHIVPADSVERQMVQRLINACPVGLFSLTQE
                     GDLRIDYRGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            complement(4358..5629)
                     /locus_tag="OO349_002114"
     CDS             complement(4358..5629)
                     /locus_tag="OO349_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR5867176.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARADLSVLLLERAEIPGSKNL
                     SGRRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRTLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWHHLRRHGLWHLAGDVIRSLRCL"
     gene            complement(5707..6069)
                     /gene="queD"
                     /locus_tag="OO349_002115"
     CDS             complement(5707..6069)
                     /gene="queD"
                     /locus_tag="OO349_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HCR5867177.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            6388..8187
                     /gene="cysJ"
                     /locus_tag="OO349_002116"
     CDS             6388..8187
                     /gene="cysJ"
                     /locus_tag="OO349_002116"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HCR5867178.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPVALAVTPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDSDVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLTGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTLIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     LETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            8187..9899
                     /gene="cysI"
                     /locus_tag="OO349_002117"
     CDS             8187..9899
                     /gene="cysI"
                     /locus_tag="OO349_002117"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HCR5867179.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMVKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            9973..10707
                     /gene="cysH"
                     /locus_tag="OO349_002118"
     CDS             9973..10707
                     /gene="cysH"
                     /locus_tag="OO349_002118"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HCR5867180.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            10971..11123
                     /locus_tag="OO349_002119"
     CDS             10971..11123
                     /locus_tag="OO349_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HCR5867181.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            11317..12408
                     /locus_tag="OO349_002120"
                     /pseudo
     CDS             11317..12408
                     /locus_tag="OO349_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311642.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
     gene            complement(12554..13591)
                     /gene="iap"
                     /locus_tag="OO349_002121"
     CDS             complement(12554..13591)
                     /gene="iap"
                     /locus_tag="OO349_002121"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HCR5867182.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKNWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGIDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTPAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            13843..14751
                     /gene="cysD"
                     /locus_tag="OO349_002122"
     CDS             13843..14751
                     /gene="cysD"
                     /locus_tag="OO349_002122"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HCR5867183.1"
                     /translation="MDQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            14753..16180
                     /gene="cysN"
                     /locus_tag="OO349_002123"
     CDS             14753..16180
                     /gene="cysN"
                     /locus_tag="OO349_002123"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HCR5867184.1"
                     /translation="MNTVLAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPDNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGLTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGMLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEVFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAASSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            16180..16785
                     /gene="cysC"
                     /locus_tag="OO349_002124"
     CDS             16180..16785
                     /gene="cysC"
                     /locus_tag="OO349_002124"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HCR5867185.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLSVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            16835..17158
                     /locus_tag="OO349_002125"
     CDS             16835..17158
                     /locus_tag="OO349_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HCR5867186.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFLYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGSLF
                     MWLLG"
     gene            17352..17663
                     /gene="ftsB"
                     /locus_tag="OO349_002126"
     CDS             17352..17663
                     /gene="ftsB"
                     /locus_tag="OO349_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HCR5867187.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAALQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            17682..18392
                     /gene="ispD"
                     /locus_tag="OO349_002127"
     CDS             17682..18392
                     /gene="ispD"
                     /locus_tag="OO349_002127"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HCR5867188.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVTRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDVQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            18392..18871
                     /gene="ispF"
                     /locus_tag="OO349_002128"
     CDS             18392..18871
                     /gene="ispF"
                     /locus_tag="OO349_002128"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HCR5867189.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            18868..19917
                     /gene="truD"
                     /locus_tag="OO349_002129"
     CDS             18868..19917
                     /gene="truD"
                     /locus_tag="OO349_002129"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HCR5867190.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHSREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            19898..20659
                     /gene="surE"
                     /locus_tag="OO349_002130"
     CDS             19898..20659
                     /gene="surE"
                     /locus_tag="OO349_002130"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HCR5867191.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     QDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            20653..21279
                     /gene="pcm"
                     /locus_tag="OO349_002131"
     CDS             20653..21279
                     /gene="pcm"
                     /locus_tag="OO349_002131"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HCR5867192.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            21419..22558
                     /gene="nlpD"
                     /locus_tag="OO349_002132"
     CDS             21419..22558
                     /gene="nlpD"
                     /locus_tag="OO349_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HCR5867193.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKMGTMSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSA
                     NKMLPNNKPTATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            22621..23613
                     /gene="rpoS"
                     /locus_tag="OO349_002133"
     CDS             22621..23613
                     /gene="rpoS"
                     /locus_tag="OO349_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HCR5867194.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRK"
     gene            complement(23733..24140)
                     /locus_tag="OO349_002134"
     CDS             complement(23733..24140)
                     /locus_tag="OO349_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR5867195.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDVADKRRRFVWLTAEGEKLLAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMST"
     gene            24287..24880
                     /locus_tag="OO349_002135"
     CDS             24287..24880
                     /locus_tag="OO349_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HCR5867196.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            24880..25827
                     /locus_tag="OO349_002136"
                     /pseudo
     CDS             24880..25827
                     /locus_tag="OO349_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
     gene            complement(26100..26663)
                     /locus_tag="OO349_002137"
     CDS             complement(26100..26663)
                     /locus_tag="OO349_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5867197.1"
                     /translation="MRKAPQGSFPADGKEPIIERIFRLVERYPSRNAAARAWGINEGT
                     LKNYYNRRDYAPIPRPHQLKKIAESENVTLEWLMTGEGDVELKDSKEPKKENADNVDA
                     QILMFLSFLKPQEKQQVADVLGRKGAEQLLILLDEHIQELHALVGVRRAIALSLGNLP
                     DEKVREIYALSEAKGDHLNLPQKAASA"
     gene            26816..27067
                     /locus_tag="OO349_002138"
     CDS             26816..27067
                     /locus_tag="OO349_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5867198.1"
                     /translation="MRQREDSDRDWPSLKIQLALKDNGWSSFRELSLHAGYAQDSLKA
                     VVRTPNKKYERVISDAIGVPPEIIWPSRYRNDGFIRKAS"
     gene            27069..29165
                     /locus_tag="OO349_002139"
     CDS             27069..29165
                     /locus_tag="OO349_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5867199.1"
                     /translation="MQSYVTVNDLLGVPGMPATTKGIRQALQRFSRDLGDVSRRREGT
                     KAIEYHIDCLPEITRKALRERYVEQLVATENNVSEVKAVTRKTRNPDAVQAIEAYRGS
                     PQLMEERLNALTENQRWVSEARAALVVEVLKLESAGNPGRLKAINFLVEKARKGELPE
                     RLQQAAVNANAKRGANRTISRDPLYQWVLKYNQSQNAAERLLLLAPGKRDEIKPEEIS
                     WLPEFLAQYRQVNGRPMSEAYEDFVAEWQRRHADEPYMLEVMPSYDVVRYAMKKLPEV
                     VKQKGRVTGSEYRQLEGFTRRDWTAMPVNYVWIGDGHGMKLKCAHPIHGRPFSPEVTF
                     VIDGGTRFVVGWSLDLAENVFAVAGAIQHGIRNHGKPFLYYSDNGSGETADMLDKEVV
                     GILPRLGIKHPTGIAGNPQGRGIIERLNRTLPMRIARRYRTYFGKGADRESLRVLNRD
                     LRSAFNALQQDKPLNDRQKAAMRELPSWAELIEAIREGVEWYNNRPHSELPMKPNGRH
                     YSPTEFRKKRQAEEDTEIEWLSDLELRDMFRPMVERPVRRCEIQWLNNIYYAPELRDE
                     HGRKVLISYDIHDAERITVRRKDGSFICEAIWNGNKRAAFAVSAEYHKQQQRIKGMRK
                     RAEEKIRDAEDEGIQILEHKQAEPWLSNVYRPVGNVVAVQQPEYEEEHDEEFERDFRL
                     GMQKLFAMQEEDDPLA"
BASE COUNT         7155 a         7241 c         7923 g         6938 t
ORIGIN      
        1 cgacatcacc atgctgggcg gtcactcctc tcatagctat cagaacggca ccaacatgta
       61 cttcgtctat gactacaacg ttgttgactg taagccggaa gaggaaatcg acaagtacca
      121 caatccgctc aacaaaatca tctgcgaaga aaccattcgc ctcggcggtt cgatggtgca
      181 ccaccatggc attggtaaac atcgcgttca ctggagcaag ctggaacacg gcagcgcgtg
      241 ggcgttgctg gaagggctga aaaagcagtt cgatcctaac ggcattatga atacgggtac
      301 tatctatccg attgaaaaat aatgtatcag gcagcgttcc gcgatgacgg ggcgttgcct
      361 tttcggcttc tcaggcgaga agccgttctt attaccggac aatgaagggg taaagatgaa
      421 cacttcaccg gtgcgaatgg atgatttacc gcttaaccgt tttcactgtc gaattgctac
      481 gctcactttc ggcgcacacc tgaccgacgg ttatgttcta ggcgtcattg gttacgccat
      541 tattcaactt acgcccgcca tgcaactgac gccgtttatg gcgggaatga tcggcggctc
      601 ggcgctcctt ggtttgtttc ttggcagcct ggttcttggg tggatctccg accatattgg
      661 tcggcaaaaa atcttcacct tcagcttttt gctgattacg ctcgcttcgt tcttgcaatt
      721 ttttgccacc acgccagagc atcttattgg gctgcgcatt ttgatcggca ttggtctggg
      781 aggcgattac tcagtaggtc acaccttgct ggctgaattt tccccgcgcc gccatcgcgg
      841 tattttgctg ggcgcattca gcgtggtgtg gaccgtaggc tatgtgttgg caagtattgc
      901 cggacatcac tttatttccg aaaacccgga ggcctggcgc tggttgctgg catcggcagc
      961 tctgcccgcg ttgttgatta cgttattacg ctggggaacg ccagaatcgc cacgttggct
     1021 actgtgccag gggcgttttg cagaagctca cgctatcgtg catcgctatt ttggtcccca
     1081 tgttttactg ggcgatgaag tggtaacggc gacccataaa cacatcaaaa ccttgttctc
     1141 ttcgcgttac tggcggcgca cggcgtttaa cagcgtcttc tttgtctgcc tcgtaatccc
     1201 atggtttgtg atttatacct ggctgccaac tatcgcccag actattggtc tggaagatgc
     1261 gctgactgcc agcctgatgc ttaatgcgtt gttaattgtg ggcgcgctgc tgggattagt
     1321 tctgacgcac ctgctggcac atcgcaaatt tttgctggga agttttttgc tgctggcggc
     1381 aacgctggta gtaatggcct gtttgccttc cggcagttca ttaacgctgc tgctttttgt
     1441 tctcttcagc accaccattt cggcagtcag taatctggtg ggcattttgc ctgcggaaag
     1501 ttttcctact gacattcgct cgctgggcgt cggttttgcc accgccatga gtcgacttgg
     1561 cgcggcggta agtactggcc tgctgccgtg ggtgctggcg cagtggggaa tgcaagtcac
     1621 cttattgctc ctagcgacag tgttgttggt tggttttgtt gtgacctggc tatgggcacc
     1681 agaaactaaa gccctcccgc tggtggcggc gggaaatgta ggaggtgcga atgaacattc
     1741 tgttagcgtt taaagccgaa ccggatgccg gaatgctggc ggaaaaagag tggcaggcgg
     1801 cggctcaggg taatagcgga ccggatgttt cgctactgcg aagtttactc ggtgctgatg
     1861 aacaggccgc tgccgcgctg ctgttagcgc agagaaaaaa cggtacgccg atgtctttaa
     1921 ccgcgttgag catgggggat gaacgggcgt tgcactggct gcgctatctc atggctcttg
     1981 ggtttgagga agctgtattg ctggagacgg cggcagatct gcgctttgcg ccggaatttg
     2041 tcgctcgcca tattgccgaa tggcagcgtc agaatccgct ggatctgata attaccggct
     2101 gccaaagcag cgaagggcag aatgggcaaa cgccattttt gctggcggaa atgctgggct
     2161 ggccctgctt cacccaggtg gagcgtttca ctctcgacgc gctgtttatc accctcgaac
     2221 agcgtactga acatgggttg cgctgttgcc gggttcgctt gcctgcggtg attgccgtgc
     2281 gtcagtgcgg tgaagtggcc ttgccagtac cgggaatgcg ccagcgaatg gctgcgggga
     2341 aagcagaaat tatccgtaaa actgttgctg ccgaaatgcc agcgatgcag tgtctgcaac
     2401 tggcaagagc agagcaacga cgcggagcga cgctgattga tgggcaaacg gtagcagaaa
     2461 aagctcaaaa gctatggcag gactatttac gccaaaggat gcagccatga atatcgcaat
     2521 tgtgacaata aatcaggaga atgccgcaat agcgagctgg ctggcggcgc aggatttcag
     2581 cggttgtacg ctggcgcact ggcaaataga acctcagccc gtagtggcgg aacaggtgct
     2641 ggatgcgatg gtggaacagt ggcagcgaac gcctgcggac gttgttctct ttccgccggg
     2701 aacgtttggc gatgaactgt cgacccggct ggcctggcgt ttacatggtg caagcatctg
     2761 tcaggtgact tcgttagata ttccaaccgt aagcatgcgc aaatcccact ggggaaatgc
     2821 gttaacggcg acgctgcaaa cagaaaaacg cccgttatgc ctttccctgg cgcgtcaggc
     2881 gggagcagcg aaaaacgcca cgttgccctc tggaatgcag caactgaaca ttgtcccagg
     2941 tgcgcttccc gactggctgg ttagcacaga ggatctgaaa aatgttagcc gcgacccatt
     3001 agctgaggcc agacgggtgc tggtggtggg acagggcgga gaggcagata accaggaaat
     3061 cgttatgctg gcagaaaagc tgggagccga agtgggctac agtcgggcgc gagtgatgaa
     3121 cggtggcgtt gatgctgaaa aagtgattgg tatttccgga catctgctgg cacctgaggt
     3181 gtgcattgtt gttggcgctt ccggcgcagc agcgttaatg gcgggcgtac gcaatagcaa
     3241 gtttatggtg gcgattaatc atgacgccag cgcagcggta ttttcacagg cagatattgg
     3301 cgtggttgat gactggaaag tggtgctgga ggcactggtg acaaacattc acgcagaata
     3361 ccaataacgt gctgccggat gatcctgcct acggttctgg actggggacc tgtaggcctg
     3421 ataagacgcg ttaagcgtcg catcaggcgc tgaatgtcgg atgtggcgtg cggccaacaa
     3481 atcaaaacaa attcaattgg ttacgattat cccgtcaaag caattttttc gctaacgtcc
     3541 agactccggt attcgtggtg gaaagcgcca cgacacccgc gttaatcgca ttacgcgcat
     3601 cttcttcatc gcataccagc ccgccagcaa tcagcggttg gcggattttt tccgtcaccc
     3661 agcccagcac tttgggcatg cagcctggca ggatctcaat acaatccgga ttcgattgcg
     3721 caacttactt atcaatgttg tgaaacgaaa tcgaatcaac aataaacagg tgatgaatac
     3781 agaaaaaaac ttccgctctt gccgctttta gcatcgatgc tttggtgctg ataattccat
     3841 ccgcctcggt caccagtttc aaaaactgaa tcaccacttc tttatttgac gcgccttcca
     3901 gcaaatcaac atgaataaaa gcatatttcc cggcgttctt aatcttttta acgatattac
     3961 tgatggtgca gatattgccg tacaacacgg aaataaactg gcattcagaa tcgatggcca
     4021 gttgcaggct ggcattatct ttaacggcag caatcaccgg attctggcgg agcaggtgta
     4081 agaggggcat aaattatcct tattatttat ccaaagcggt aggtgatgcc gaatccggaa
     4141 ggcggatagc gccactgttg tagtgtggat tcgtcgcaca gcaaacggca ggtgccacac
     4201 tccaggcagc cgcgatagtc aatacgtaag tcaccttcct gtgtgagaga aaaaagacct
     4261 accggacagg cgttaatcaa ccgttgtacc atctgacgct caacggagtc agccggaaca
     4321 atgtgcggcg catcagcaac gcgccagaga ttacgggcta cagacatcgc agactcctga
     4381 taacatcgcc cgccagatgc cacaggccat gacgacgtaa atgatgccag aataactggc
     4441 gcagcggtgg aacaggttta tcaccctgat cccataaatc gcgggaaata tcctgcatta
     4501 acgcaggcca cgcccgatac cagccagggc gttgcaaaag cgccggaaca tgctgataac
     4561 gttgtagaac atcccatagc aggctgcgct ctacgttgtg atgataaagc gcaaacagat
     4621 tttgcggctc gcggtgctgg caggcgctta ttagcgtttg tgccgccgcc tgtgcgccag
     4681 tcagcgccat atccatgccg cgcacggaaa ttccggtatt gacgcaactg cgcaacgcat
     4741 cgcccaccag cagccagccg ttaccggcgt attgcaccgg catactgtgc aagccacctt
     4801 ctggcaccag atgcgcacca tactccagtg attccgtgtt tttgataagc gtgcgcactg
     4861 ccggatgcgc tttaaagcga gtcagcagct cgcttgccgg aacacgactt tgcgtaaggg
     4921 aagagagcgg gcaaacaatc cctaacgaga gcgtttgttg attagtataa agaaatgcgc
     4981 cgccgggtaa gtcatcacag attccgccgc tgaacagcaa cgctgcgcct tcgttattct
     5041 ccagatgaaa acgttcttca atagcggatg tttccagcga cagcacttct ttgatcccca
     5101 acgccatcgc ttcgccagca ggacgagtca ctaacccgtg acgttcagcc aggacgctgt
     5161 tggcaccttc tgccagcacc acataacggg cgcggagaat atcgtcacca caaataacgc
     5221 cacagactct gccgttttct tcatacagtg catccaccgt cgctccgggg atgcattcga
     5281 caccttcttt ttcggcttcg gcaaccagcc acggatcgaa ccgtgcacgt aatacactcc
     5341 aggattcacc gccgggctgt aagctggaaa acgtcgttgc gccatccggc gttaacaggg
     5401 aaaggctttc gtgagtgatg cgtcgttcaa gaggcgcggt cagatgaaat tgcgggagga
     5461 gttccgcgag tgcatgggta tataaccgcc tgccggaaag atttttgctg ccggggattt
     5521 cagcgcgttc gagtaacaaa acggataaat ccgctcgcgc gcagcgtaac gcgcaagcgg
     5581 tgcctgcaat accggcacca ataataataa tgtcgcagtc gtcttccatg tcatgcccca
     5641 gatatccaga acacaacacc ctaacatagc gttactaaag ggaaattgac cgccgacaca
     5701 ctcttatcat tcgccgcgat agatacaccc tgcggtgcag gtttctttta ccatcaccgc
     5761 acttaacagc ggcacaacgg gtttaacctg atcccaaatc cattttgcta aaacttcgct
     5821 ggttggattt tccagacctg gaatatcatt gagataatgg tgatcgaggc gctcataagt
     5881 tggcttaaac gccgctttca gttcagcgaa atcgataatc cagcccgtat gcggatcgac
     5941 ttccccggta atttccagtc gcaccataaa ggaatgcccg tgcaggcgac cacatttatg
     6001 cccttccggg acatgtggta agcggtgagc ggcttcgaag gtgaaatctt taaataacgt
     6061 ggtggacatc ataatttctc tacaggacga taaaaccgcc gtagagtacc ggaaaggctg
     6121 ttttttcgca ttatctaaaa cgactttgtt gcgcaaaatc gctgatttat cttaatgatt
     6181 ggctaaattc atttgttttt cattaggttg gttaatctat tttgttgtta aagactattg
     6241 ctaaaacagg ttagtcgatt tggttattag ttatcgctat cccgtcttta atccacaccg
     6301 ttcaccccgt taaccttacc ttctcttctg ttttatgggc gctgacaggg cgcagaaaca
     6361 gctttgctta ctggaacata acgacgcatg acgacacagg tcccaccttc cgcgttgctt
     6421 ccgttgaacc cggagcaact ggcacgcctt caggcggcca cgaccgattt aacgcctacc
     6481 cagcttgcct gggtttctgg ctatttctgg ggcgtgctca accagcagcc tgttgcgctt
     6541 gcagtgacgc cagcgcccgc cgcagaaatg ccgggtataa cgattatctc tgcttcgcaa
     6601 accggcaatg cgcgccgggt tgctgaagca ttacgcgatg atttattagc agcaaaactg
     6661 aacgttaagc tggtgaacgc gggcgactat aaattcaaac aaatcgccag cgaaaaactg
     6721 ctcatcgtag tgacgtcaac gcaaggggaa ggggaaccgc cggaagaagc cgtcgcgctg
     6781 cataagttcc tgttctccaa aaaagcgcca aagctggaaa acaccgcgtt tgccgtgttt
     6841 agcctcggcg atagctctta tgaatttttc tgccagtccg ggaaagattt tgacagcaag
     6901 ctggcggaac tggggggtga acgcctgctc gaccgtgtcg attctgatgt tgaataccag
     6961 gctgctgcca gcgagtggcg cgcccgcgtg gttgatgcgc ttaaatcgcg tgcgcctgtc
     7021 gcggcacctt cgcaatccgt cgctactggc gcggtaaatg aaatccacac cagcccgtac
     7081 agcaaagacg cgccgctggt ggctagcctc tctgttaacc agaaaattac tgggcgtaac
     7141 tctgaaaaag acgttcgcca tatcgaaatt gacttaggcg actcgggcct gcgttaccag
     7201 ccgggtgacg cgctgggcgt ctggtatcag aacgatccgg cactggtgaa agaacttgtc
     7261 gaactgctgt ggctgacagg cgatgaacct gtcactgtcg agggcaaaac gttgcctctg
     7321 aacgaagcgc tacagtggca cttcgaactg accgtcaaca ccgccaacat tgttgagaat
     7381 tacgccacgc ttacccgcag tgaaacactg ctgccgctgg tgggcgataa agcgaagtta
     7441 cagcattacg ccgcgacgac gctgattgtt gacatggtgc gtttctcccc ggcacagctt
     7501 gatgccgaag cgctaattaa tctgctgcgc ccgctgaccc cgcgtctcta ttccattgcc
     7561 tcctcgcagg cggaagtcga gaacgaagta cacgtcaccg ttggtgtggt gcgttacgac
     7621 gtggaaggcc gcgcccgtgc cggtggtgcc tccagcttcc tcgctgaccg cgtggaagaa
     7681 gagggcgaag tccgcgtatt tatcgaacat aacgataact tccgcctgcc cgctaacctg
     7741 gaaaccccgg tgattatgat tggcccaggc accggtattg cgccgttccg cgcctttatg
     7801 cagcaacgcg ccgctgacga agcgccaggt aaaaactggc tgttctttgg caatccgcat
     7861 tttacggaag acttcctgta tcaggtggag tggcagcgct acgtcaaaga gggtgtgctg
     7921 acgcggatcg atctcgcctg gtcgcgcgac caaaaagaaa aagtttacgt acaagataaa
     7981 ctgcgcgaac agggcgcgga gctgtggcgc tggatcaatg acggtgccca catttatgtc
     8041 tgcggcgacg ctaatcgcat ggcgaaagac gttgagcagg cacttctgga agtgattgcc
     8101 gaatttggtg gcatggacac cgaagcggcg gatgaatttt taagtgagct gcgcgtagag
     8161 cgccgttatc agcgagatgt ctactaatga gcgaaaaaca tccagggcct ttagtggtcg
     8221 aaggaaaact gacagacgcc gagcgcatga agcttgaaag caactacctg cgcggcacca
     8281 ttgcggaaga tttaaacgac ggtctgaccg gcggctttaa gggcgataac ttcctgctga
     8341 tccgcttcca cggcatgtat cagcaggatg accgcgacat ccgcgccgaa cgtgctgaac
     8401 agaagctgga gccgcgccac gcgatgctgt tgcgctgccg tctgccgggt ggagttatca
     8461 ccaccaaaca gtggcaggcg atcgataaat ttgccggtga aaacaccatc tatggcagca
     8521 ttcgcctgac caaccgccag acgtttcagt tccacggcat tctgaaaaag aacgtcaaac
     8581 cggtgcacca gatgctgcac tcggtcggtc ttgatgcgct ggcgactgct aacgacatga
     8641 accgtaacgt actctgcacc tcgaaccctt acgagtcgca gctgcacgca gaagcgtacg
     8701 agtgggcgaa gaagatttct gaacatctgc tgccgcgtac ccgcgcgtat gcggagatct
     8761 ggctcgatca ggaaaaagtc gccaccaccg atgaagaacc gatccttggt cagacttacc
     8821 tgccgcgtaa gttcaaaacc acggtagtga tcccgccgca gaacgatatt gatctgcacg
     8881 ccaacgacat gaacttcgtg gcgatcgcgg aaaacggcaa gctggtgggc ttcaacctgc
     8941 tggtgggcgg tgggctttct atcgaacacg gtaacaagaa aacctacgcc cgcacggcga
     9001 gtgagtttgg ctatctgccg ctagagcata cgctagcggt ggcggaagcc gtcgtgacaa
     9061 ctcagcgtga ctggggtaac cgaaccgatc gtaaaaatgc caaaaccaaa tacacactgg
     9121 agcgcgtggg ggttgagacg tttaaagcgg aagtggagcg tcgcgcgggg atcaaatttg
     9181 aaccgatccg cccgtatgaa tttacaggac gcggcgatcg tattggctgg gttaagggca
     9241 ttgatgataa ctggcacctg acgctgttta tcgaaaatgg tcgcatcctt gattatccgg
     9301 ggcgtccgct gaaaactggc ctgctggaga tcgcgaagat ccacaaaggt gatttccgca
     9361 ttacggcgaa ccagaatctg atcatcgccg gtgtgccgga aagcgagaaa gcgaagatcg
     9421 agaaaattgc caaagagagc gggttaatga atgccgtcac gccgcagcgt gaaaactcga
     9481 tggcctgcgt gtcattcccg acttgcccgc tggcgatggc ggaagcggag cgtttcctgc
     9541 cgtcttttat cgacaacatc gataatttaa tggtgaaaca tggtgtcagc gatgagcata
     9601 tcgtgatgcg tgtaacaggc tgcccgaacg gttgtggtcg cgcgatgctg gcggaagtgg
     9661 gcttggtggg taaagcaccg ggtcgctaca acctgcatct tggcggcaac cgcattggga
     9721 cacgtatccc acggatgtat aaagaaaaca tcaccgagcc ggaaatcctg gcgtcgcttg
     9781 atgaactgat agggcgctgg gcgaaagagc gcgaagcggg tgaaggcttc ggcgacttta
     9841 cggtgcgtgc gggcatcatt cgcccggtgc tcgatccggc gcgcgatttg tgggattaac
     9901 catcagcccg gtcttgtagg cctgataaga cgcgtgagcg tcgcatcagg caaggcaaac
     9961 agtgaggaat ctatgtccaa actcgatcta aacgccctga acgaactgcc gaaggtagat
    10021 cgcattctgg cgctggcaga aactaacgcc gaactggaaa aactggacgc tgaaggccgc
    10081 gttgcctggg cgctggataa tctgcccggt gaatatgtac tttcttccag ctttggcatt
    10141 caggcggcgg tgagcctgca tctggtgaat cagattcacc cggatattcc ggtgatcctc
    10201 accgacaccg gctatctgtt cccggaaacc taccgcttta ttgacgagtt aacggacaaa
    10261 ctcaagctca acctgaaagt gtaccgcgct accgaaagcg ccgcctggca ggaagcacgc
    10321 tacggcaaac tgtgggaaca gggcgttgaa ggcattgaaa agtacaatga catcaacaaa
    10381 gtcgaaccga tgaaccgggc gctgaaagaa ctgaatgcgc aaacgtggtt tgctggcctg
    10441 cggcgtgaac aatccggcag ccgtgccaat ttaccggtgc tggcaattca gcgtggcgta
    10501 tttaaagtgc tgccgattat cgactgggat aaccgaacta tttatcagta cctgcaaaaa
    10561 catggcctga aatatcaccc attatgggac gaaggatatt tatcggtggg cgatacccat
    10621 acaacccgta aatgggaacc cggcatggcg gaagaagaaa cgcgtttctt tggcttaaaa
    10681 agggaatgtg gattacacga agggtaaata ttgccggata aattaaacgc ctctgtcaga
    10741 aatgatgggg gcgtttttat tttgcgagca gcattccggc atttaaaggt gaacatcgca
    10801 gggaaaatta acaggtatag aatggcgttg ggtgcttgag gctgtctggc gttaagcatt
    10861 cgcgaggttc cagatggaca aaagccccag gcgatatttc tatcaacctg aggctcgcgt
    10921 tcgaacgtag caattcggat gttagtctct tctttgcagg gaggcaagac atgctgacaa
    10981 aatatgccct tgtggcaatc atcgtactgt gttgtacagt actgggattc acgttgatgg
    11041 taggtgactc gttatgtgag ttgagtatca gagaacgtgg tatggagttt aaggcagttc
    11101 tcgcttacga atcgaagaag tagccaccac gcgggggtaa aaccccgcgt actgaattgt
    11161 gatggtttgg tctcctgcac ccttttttta aatttatatt ttttatgtga atatatagtc
    11221 tctatttatt attatgaatt tcaaatgaaa atcattaatt tattcataat gtaaatgttt
    11281 tattggctga tatatatcat agatagtttt taaaatatgc gtaaatatcc tttaagttta
    11341 ctgaaggata aaaatattgt gactttcttt gatttctggg gaaaaaaccg acgtggcgag
    11401 aaagagggta tcgacgacta tcaccttctt tgctggcatt tgctggatgt ggccgcaatg
    11461 ggctatttaa tggttaaaag aaattgcttc gggctggctg attattttcg tcaattaggg
    11521 atttctgaca aggaacaggc agctcaattt ttcgcctggt tgctgtgctg gcacgatatt
    11581 ggaaaatttg cccgctcttt tcaacaactt tacctgcccc ctgaattcaa gattcaggaa
    11641 ggtgccagaa aaaattacga aaaaatctct cattcaacgc tgggctactg gttgtggaat
    11701 cattatttaa gtgaatgtca ggagttactt ccttcatctt cgctctctcc gcgcaaactt
    11761 aggcgagtga tagaaatgtg gatgcccgtg acgacagggc atcatggtcg gccccctgat
    11821 cgtatggatg aattggataa ttttctgcct gaagacaaag ctgcagcacg agattttctc
    11881 cttgaaatca agccactgtt tccgcgaata gagatcccca cattctggga tgatgacgag
    11941 ggcgttgaac ttataaaaca actttcctgg tatatctctg caaccgtcgt acttgcagac
    12001 tggacagggt cgtcaacgcg attttttcca cgcgtcgcac aggcaatgga tattgaagat
    12061 tactggcaaa aggctttagt tcaggctcaa aacgcattaa ccgtctttcc gccacaagca
    12121 gaaaccgcgc ctttcaccgg aattaatacg ctgtttcctt ttattgagaa tacgacaccg
    12181 ttacagcaaa aggtgctgga tctggatatc aatcagccag gaccacagtt gtttattctg
    12241 gaagatgtga ccggcgcagg taaaacagaa gcggcgctta tcctggcgca caggttgatg
    12301 gctgcgggta acgctcaggg gctgtttttt ggcctgccaa caatggcaac ggccaatgcc
    12361 atgtacgatc gactggtcaa aacctggctt gctttctatt cgccagaggt gttccccgct
    12421 ccagcggaga taaaccggcg caatacggtg cggttttccc ggaagtaaac cggacgcact
    12481 ggatgcgatg atgggtatca cttagaattc cccgcccctg cggtagaact cccagctccc
    12541 attttcaaac ccatcaagac gccttcgcca gctccttcac caacggtagc attatccgca
    12601 taacgtcacg gcagcgacgt tctattcttc caggaagtgc cttatcaata tgttgttgat
    12661 tatccagtct tacgtcatgc cagctatttc ccgccgggaa ggcaggtgtt tttgcgcgtt
    12721 gctgataacc atccttattt ccaagattcc agttagtcgc ttccaccgaa agtacagcaa
    12781 tgcccgcttt gtcgaatatt tctgcgtcat tacaacaccc agtgcctttc ggataatttt
    12841 tattcaaacc cggattggtc gttgcggcta ttccgtgact gcgtgcaatt gccagcgccc
    12901 tgtcgcgcgt taatttcctt actgcttcag gggtttttac accgctgttg aaatacaatt
    12961 tatcgccaac aattaagtta tcgagattaa tcaccagcag cgtatttttc ttttcggtgt
    13021 cactcatccg cttgagtaaa ttctcagcgc ctaattttcc ttcctcttcg ccgctggtcg
    13081 ccacaaatcg aataccatac tcggtaggcg tatttttcag gcgttctgcc agttccagca
    13141 tgacacctaa acctgcggcg ttatcatcta ttccttgtaa cgtcagtccg ccgagattgg
    13201 catctgcgtc agcatcgctc agcggggcgt aagtatccag atgcgccata atgatgatct
    13261 gctgcggcgc tttgccttca tgagcggcaa tcaccgtact tcctgtcacg ttatgccagt
    13321 tcttgcgatt atcgcgggcg gtataaatat accgactatt aaatgtccga atatcactgc
    13381 gataacccat ttgctgaaac tgttggcgaa tataatcggc agataacatt tctgctggag
    13441 ttccggtcat gcgtcccgga aagaaagtag caatatgtcg tgcctgggta ttagcaaaat
    13501 cgccaggttt aggtgacgag gcgtgtacgg ggagaataaa gcatacgccg agcgccaggg
    13561 cagcggtacg gtggcgcaat gcggaaaaca tagtgagtcc ttaaatacca tgcaaatttt
    13621 tttaccgcca tagtatgaaa ctgccgctgc gctaaaacaa tttcaaatct tcctaaacgc
    13681 ccgaaatccg gtgccttaag cactttttga tattagcttt gccaaatcgt tattccgtta
    13741 aggaactact cattctaatt ggtaatttca ttcgttctct tacgctccct atagtcgaaa
    13801 catctgatgg caagaaaata gcggtattgc aaaggaacgg ttatggatca aaaacgactt
    13861 actcacctgc ggcaactgga ggcggaaagc atccacatta ttcgcgaggt ggcggcagaa
    13921 ttctcaaatc cggtgatgct ctactctatc ggtaaagatt ccagcgtcat gctgcatctg
    13981 gcgcgcaagg cgttttatcc aggtacgctg cctttcccgt tgctgcatgt cgataccggc
    14041 tggaaattcc gcgagatgta tgagttccgc gatcgtacgg cgaaagccta cggctgcgag
    14101 ctgctggtgc ataaaaaccc ggaaggcgtg gcgatgggga ttaatccatt cgtgcacggc
    14161 agcgcgaaac ataccgatat tatgaaaact gaaggcctga aacaggcgct gaacaaatac
    14221 ggttttgatg ccgccttcgg tggtgcgcgc cgtgacgaag agaaatcccg cgctaaagag
    14281 cgaatctact ctttccgtga ccgcttccat cgctgggatc cgaaaaatca gcgcccggag
    14341 ctgtggcaca actacaacgg gcaaattaac aaaggcgaaa gcatccgcgt cttcccgctc
    14401 tctaactgga ccgagcagga tatctggcaa tacatctggc tggaaaatat cgacattgtg
    14461 ccgctgtatc tcgctgcgga acgtccggtt ctggaacgcg acggtatgtt gatgatgatt
    14521 gatgacaacc gtatcgacct gcaaccgggc gaagtgatca aaaaacggat ggtgcgtttc
    14581 cgtacgctgg gctgctggcc gctgaccggt gcggtggagt caaatgcaca aacactgccg
    14641 gaaatcatcg aagagatgct ggtttccacc accagtgaac gtcaggggcg cgtgattgac
    14701 cgcgaccagg cgggatccat ggagctgaaa aaacgtcagg ggtattttta agatgaacac
    14761 cgtacttgca caacaaatcg ccaatgaagg cggcgtcgaa gcctggatga ttgcgcaaca
    14821 acataaaagc ctgctgcgtt ttctgacctg tggtagcgtc gatgacggca aaagtaccct
    14881 gattggtcgt ctgctgcacg atacccgcca aatctacgaa gatcagctct catcgctgca
    14941 taacgacagt aagcgtcacg gcacccaggg cgaaaagctg gatctggctc tgctggtgga
    15001 cggcctgcaa gctgagcgcg aacagggcat cactattgat gtggcctacc gctatttctc
    15061 taccgagaag cgtaaattta ttatcgccga caccccaggg cacgagcagt acactcgcaa
    15121 tatggcgact ggcgcatcga catgtgaact ggcgatctta ctgattgatg cccgtaaagg
    15181 cgtgctcgat caaacccgtc gtcacagttt tatctccaca ctgttgggga tcaaacatct
    15241 ggtcgtggcg atcaacaaaa tggatctggt ggattacagt gaagagacgt tcacccgtat
    15301 tcgtgaagat tatctgacct ttgccgggca gctgccggat aatctggata tccgctttgt
    15361 gccgctctcc gcactggaag gtgacaacgt ggcttcgcaa agtgaaagta tgccgtggta
    15421 cagcggtctg acactgctcg aagtgctgga aaccgtggag atccagcgag tggtggatgc
    15481 ccagccaatg cgcttcccgg tgcagtacgt taaccgccca aatctcgatt ttcgtggcta
    15541 cgccggaatg ctggcatccg gtcgcgtgga agtcgggcaa cgtgtaaaag tgctgccctc
    15601 tggtgtggaa tcaaacgtcg cgcggatcgt gacttttgat ggtgatcgcg aagaagtctt
    15661 tgccggagaa gcgatcaccc tggtgctgac ggatgagatc gatatcagcc gtggtgatct
    15721 gctgctggcg gcagacgaag cgttaccggc ggtgcagagt gcgtcggtgg atgtggtatg
    15781 gatggcggaa cagccgcttt ccccgggcca gagttatgac atcaaaattg ccggtaagaa
    15841 gacgcgtgct cgtgttgatg gcattcgtta tcaggttgat attaataacc ttacccaacg
    15901 cgaagttgaa aacctgccgc tgaacggcat cggcctggtg gatctcactt ttgacgagcc
    15961 actggtgtta gatcgctatc agcaaaatcc ggttaccggt gggctgattt ttatcgatcg
    16021 cctgagcaat gtgaccgtgg gtgccggtat ggtgcacgag ccagttagcc aggcaacagc
    16081 tgcgtcatct gaattcagtg cattcgaact ggaattgaat gccctggttc gtcgccactt
    16141 tccgcactgg ggcgcgcgcg atttgctggg ggataaataa tggcgctgca tgacgaaaac
    16201 gtcgtctggc atagtcatcc ggtcactgtg caacaacgcg aactacacca cggtcatcgt
    16261 ggtgtagtgc tgtggtttac cggcctctcc gggtccggta aatcaacggt cgccggggcg
    16321 ctggaggagg cgttacataa actcagcgtc agtacgtatc tgctggatgg cgacaatgtt
    16381 cgccacggat tatgcagcga tctcggtttt agcgatgccg atcgtaaaga gaatatccgt
    16441 cgcgtcggtg aagtggcgaa tttgatggtt gaagccggac tggtggtgct gaccgcattt
    16501 atctcgccac accgcgccga acgccagatg gttcgcgaac gcgtaggaga agggcgcttt
    16561 attgaagtgt ttgtcgatac gccgctggcg atttgcgaag cccgcgatcc aaaaggctta
    16621 tataagaaag cgcgtgccgg tgaactgcgc aactttacgg gaatagattc cgtttacgaa
    16681 gcgcctgaat cggcagaaat tcatctcaat ggtgaacaat tagtaacaaa tttggtacag
    16741 caattattag atctgttgag acagaacgat attatcagat cctgagacac caccgggttt
    16801 ccatgcccgg ttcgtcaaag taacaggatc aaatatgcgt aatagccata acattacact
    16861 aacaaataac gacagcctta ctgaggatga agaaactaca tggtcactgc ctggtgccgt
    16921 ggtcggtttt atctcctggt tatttgcgct ggcgatgccg atgttgattt atggctctaa
    16981 cacgctgttc ttctttctct acacctggcc tttctttctg gcactgatgc ctgtcgcggt
    17041 agtggtgggg attgcgctgc attcattgat ggacggaaag ctacgctaca gtattgtttt
    17101 caccctggtg acagtaggca ttatgtttgg ctcactgttt atgtggctac tgggctaatt
    17161 tgtactttct tgtctcctct gttcataatt caaaccgtaa ctaataatga gattatgttc
    17221 tgcacgccct gggtatacgt aacaatggac aaatgtggta catttgcccg cgttgtcgcg
    17281 gtatccccaa cagaggatgt agagtcgtct tcggatgcat gggatgatga tgcagttttt
    17341 cagggggcag gatgggtaaa ctaacgctgc tgttgctggc tattctggtc tggctacagt
    17401 attcgctgtg gttcggtaag aacggtatac atgactatac ccgcgtcaat gatgatgtgg
    17461 cggcactgca agctacaaac gcgaaactta aagcgcgaaa cgatcaactt tttgccgaaa
    17521 ttgacgatct caatggcggc caggaggcgc tcgaagagcg tgcgcgtaat gaactcagca
    17581 tgaccaggcc gggcgaaact ttttatcgtc tggtgcctga cgcgtcgaag cgcgcacagt
    17641 ctgcggggca aaacaatcga taaatcagcc cgggaattaa catggcaacc actcatttgg
    17701 atgtttgcgc cgtggttccg gcggccggat ttggccgtcg aatgcaaacg gaatgtccta
    17761 agcaatatct ctcaatcggt aatcaaacca ttcttgaaca ctcggtgcat gcgctgctgg
    17821 cgcatccccg ggtgacacgt gtcgtcattg ccataagtcc tggcgatagc cgttttgcac
    17881 aacttcctct ggcgaatcat ccgcaaatca ccgttgtaga tggcggtgat gagcgtgccg
    17941 attccgtgct ggcaggtctg aaagccgctg gcgatgtgca gtgggtattg gtgcatgacg
    18001 ccgctcgtcc ttgtttgcat caggatgacc tcgcgcgatt gttggcgttg agcgaaacca
    18061 gccgcacggg agggatcctc gccgcaccag tgcgcgatac tatgaaacgt gccgaaccgg
    18121 gcaaaaatgc cattgctcat accgttgatc gcaacggctt atggcacgcg ctgacgccgc
    18181 aatttttccc tcgtgagctg ttacatgact gtctgacgcg cgctctaaat gaaggcgcta
    18241 ccattaccga cgaagcctcg gcgctggaat attgcggatt tcatccgcag ttggtcgaag
    18301 gccgcgcgga taacattaaa gtcacgcgcc cggaagattt ggcactggcc gagttttacc
    18361 tcacccgaac catccatcag gagaatacat aatgcgaatt ggacacggtt ttgacgtaca
    18421 cgcctttggc ggtgaaggcc caattatcat tggtggcgta cgcattcctt acgaaaaagg
    18481 attgctggcg cattctgatg gcgacgtggc gcttcatgcg ttgaccgatg cattgcttgg
    18541 cgcggcggcg ctgggggata tcggcaaact gttcccggat accgatccgg catttaaagg
    18601 tgccgacagc cgcgagctgc tacgcgaagc ctggcgtcgt attcaggcga agggttatac
    18661 ccttggcaac gtcgatgtca ctatcatcgc tcaggcaccg aagatgttgc cgcacattcc
    18721 acaaatgcgc gtgtttattg ccgaagatct cggctgccat atggatgatg ttaacgtgaa
    18781 agccactact acggaaaaac ttggatttac cggacgtggg gaagggattg cctgtgaagc
    18841 ggtggcgcta ctcattaagg caacaaaatg attgagtttg ataatctcac ttacctccac
    18901 ggtaaaccgc aaggcaccgg gctgctgaaa gccaatccgg aagactttgt ggtggtggaa
    18961 gatttgggct ttgagcctga tggtgaaggt gagcatattc tggttagaat cctcaaaaac
    19021 ggctgcaata cccgttttgt ggcggatgca ctggcgaaat tcctgaaaat tcattcccgt
    19081 gaagtcagct tcgctgggca aaaagacaaa catgctgtaa cggaacagtg gttatgtgct
    19141 cgcgtgccgg gcaaggaaat gcccgatctg agcgcctttc aactggaagg ctgccaggtg
    19201 ctggagtatg cgcggcacaa gcgcaagctg cgtttagggg cgctgaaagg taacgccttt
    19261 accctggttc tgcgcgaagt gagcaatcgc gatgacgttg aacaacgtct gatcgatatt
    19321 tgcgtaaaag gtgtaccgaa ctacttcggt gcccaacgtt ttgggattgg cggtagcaac
    19381 ttgcagggcg cgctgcgctg ggcgcaaacc aatactccgg tgcgcgatcg caataaacgg
    19441 agtttttggt tgtcggcagc ccgcagtgcg ttgtttaatc agattgttgc tgagcgcctc
    19501 aaaaaagcag acgttaatca agttgttgac ggcgatgcgc tacaattagc cggacgtggt
    19561 agctggtttg tagcaaccac cgaagaactg gcggaattac agcgtcgcgt caacgataaa
    19621 gagctgatga taaccgccgc attgccgggc agtggcgaat ggggaactca gcgtgaagcg
    19681 ctggcattcg aacaagcagc tgtcgccgca gaaactgaat tacaagcttt actggtgcgc
    19741 gaaaaagttg aagccgcgcg cagagcgatg ctgctgtatc cgcaacaatt aagctggaac
    19801 tggtgggatg acgtcaccgt agagatccgt ttctggcttc cggcgggtag ttttgcaacc
    19861 agcgttgtca gggaacttat caacacaaca ggtgattatg cgcatattgc tgagtaatga
    19921 tgacggggta catgcacccg gtatacaaac gctggcgaaa gccttgcgtg agtttgctga
    19981 cgttcaggtg gtcgcccccg atcgtaaccg cagcggcgct tcaaattctc tgacactgga
    20041 atcctccctg cgcacgttta cctttgaaaa tggtgatatt gctgtgcaaa tgggaacccc
    20101 gaccgattgt gtctatcttg gcgtgaatgc tctgatgcgt ccgcgcccgg acattgttgt
    20161 gtccggaatt aacgccgggc cgaatctggg ggatgatgtt atttattccg gtacggtagc
    20221 cgccgcgatg gaaggccgtc atttaggttt tccggcgctg gccgtctcgc ttgacgggca
    20281 taaacattac gacactgccg cggcggtaac ctgttcaatt ttgcgcgcac tgtgtaaaga
    20341 gccgctgcgc accggacgta ttcttaatat taacgttcag gatttaccct tggatcaaat
    20401 caaaggtatt cgcgtgacgc gctgcggtac acgacatccg gcagatcagg tgatcccgca
    20461 gcaagatccg cgcggcaata cgctgtactg gattggcccg ccgggcggta aatgtgatgc
    20521 cggtccgggg accgattttg ctgcggtaga tgagggctat gtctccatca cgccgctgca
    20581 tgtggattta actgcgcata gcgcgcaaga tgtggtttca gactggttaa acagcgtggg
    20641 agttggcacg caatggtaag cagacgcgta caagcacttc tggatcaatt acgtgcgcaa
    20701 ggtattcagg atgagcaggt gctgaatgca cttgccgccg tgccgcgtga aaaattcgtt
    20761 gatgaagcgt ttgaacaaaa agcctgggac aatatcgcct tgccgatagg tcaggggcag
    20821 acaatttcgc agccgtatat ggtggcgcga atgaccgaat tactcgagct gacgccgcag
    20881 tcgcgggtgc tggaaattgg cactggttcg ggatatcaaa cggcaatcct ggcgcatctt
    20941 gtccagcatg tttgctcggt tgaacggatt aaaggcttgc agtggcaggc acgtcgccgc
    21001 ctgaaaaatc ttgatttaca taatgtttca acccgtcatg gtgatggatg gcaaggttgg
    21061 caggcacgtg cgccgtttga cgctatcatt gttacggcgg caccgccgga aattccaact
    21121 gcgctaatga cgcagctgga cgaaggcggg attctcgtct tacccgtagg ggaggagcac
    21181 cagtatttga aacgggtgcg tcgtcgggga ggcgaattta ttatcgatac cgtggaggcc
    21241 gtgcgctttg tccctttagt gaagggtgag ctggcttaaa acgtgaggaa atacctggat
    21301 ttttcctggt tattttgccg taggtcagcg tatcgtgaac atcttttcca gtgttcagta
    21361 gggtgccttg cacggtaatt atgtcactgg ttattaacca atttttcctg ggggataaat
    21421 gagcgcggga agcccaaaat tcaccgttcg ccgcattgcg gctttgtcac tggtttcgct
    21481 atggctggca ggctgttctg acacttcaaa tccaccggca ccggtcagct ccgttaatgg
    21541 caatgcgcct gcaaatacta attctggtat gttgattacg ccgccgccga aaatggggac
    21601 gatgtctaca gcgcagcaac cgcaaattca gccggtacag cagccacaaa ttcaggctac
    21661 tcaacaaccg caaatccagc cagtgcagcc agtagctcag cagccggtac agatggaaaa
    21721 cggacgcatc gtctataacc gtcagtatgg gaacattccg aaaggcagtt atagcggcag
    21781 tacctatacc gtgaaaaaag gcgacacact tttctatatc gcctggatta ctggcaacga
    21841 tttccgtgac cttgctcagc gcaacaatat tcaggcacca tacgcgctga acgttggtca
    21901 gaccttgcag gtgggtaatg cttccggtac gccaatcact ggcggaaatg ccattaccca
    21961 ggccgacgca gcagagcaag gagttgtgat caagcctgca caaaattcca ccgttgctgt
    22021 tgcgtcgcaa ccgacaatta cgtattctga gtcttcgggt gaacagagtg ctaacaaaat
    22081 gttgccgaac aacaagccaa ctgcgaccac ggtcacagcg cctgtaacgg taccaacagc
    22141 aagcacaacc gagccgactg tcagcagtac atcaaccagt acgcctatct ccacctggcg
    22201 ctggccgact gagggcaaag tgatcgaaac ctttggcgct tctgaggggg gcaacaaggg
    22261 gattgatatc gcaggcagca aaggacaggc aattatcgcg accgcagatg gccgcgttgt
    22321 ttatgctggt aacgcgctgc gcggctacgg taatctgatt atcatcaaac ataatgatga
    22381 ttacctgagt gcctacgccc ataacgacac aatgctggtc cgggaacaac aagaagtgaa
    22441 ggcggggcaa aaaatagcaa ccatgggtag caccggaacc agttcaacac gcttgcattt
    22501 tgaaattcgt tacaagggga aatccgtaaa cccgctgcgt tatttgccgc agcgataaat
    22561 cggcggaacc aggcttttgc ttgaatgttc cgtcaaggga tcacgggtag gagccacctt
    22621 atgagtcaga atacgctgaa agttcatgat ttaaatgaag atgcggaatt tgatgagaac
    22681 ggagttgagg tttttgacga aaaggcctta gtagaagagg aacccagtga taacgatttg
    22741 gccgaagagg aactgttatc gcagggagcc acacagcgtg tgttggacgc gactcagctt
    22801 taccttggtg agattggtta ttcaccactg ttaacggccg aagaagaagt ttattttgcg
    22861 cgtcgcgcac tgcgtggaga tgtcgcctct cgccgccgga tgatcgagag taacttgcgt
    22921 ctggtggtaa aaattgcccg ccgttatggc aatcgtggtc tggcgttgct ggaccttatc
    22981 gaagagggca acttggggct gatccgcgcg gtagagaagt ttgacccgga acgtggtttc
    23041 cgcttctcaa catacgcaac ctggtggatt cgccagacga ttgaacgggc gattatgaac
    23101 caaacccgta ctattcgttt gccgattcac atcgtaaagg agctgaacgt ttacctgcga
    23161 accgcacgtg agttgtccca taagctggac catgaaccaa gtgcggaaga gatcgcagag
    23221 caactggata agccagttga tgacgtcagc cgtatgcttc gtcttaacga gcgcattacc
    23281 tcggtagaca ccccgctggg tggtgattcc gaaaaagcgt tgctggacat cctggccgat
    23341 gaaaaagaga acggtccgga agataccacg caagatgacg atatgaagca gagcatcgtc
    23401 aaatggctgt tcgagctgaa cgccaaacag cgtgaagtac tggcacgtcg attcggtttg
    23461 ctggggtatg aagcggcaac actggaagat gtaggtcgtg aaattggcct cacccgtgaa
    23521 cgtgttcgcc agattcaggt tgaaggcctg cgccgtttgc gcgaaatcct gcaaacgcag
    23581 gggctgaata tcgaagcgct gttccgcaag taagtaagca tctgtcagaa aggccagtct
    23641 caagcgaggc tggccttttt cttttgtttg ggaagcttgt gtttttgtcg cctggataag
    23701 acacgtcagc gtcctacccg gcaatcagca ttctatgtgc tcatcatctt gcgcaccaac
    23761 tgcataaaca gctcctgctc ttctccactc aaacgcccca aaaattcctc atccacgctg
    23821 tcaccaatag gtatcgccgc agcaagtagc ttttctcctt cggcagtcag ccagacaaag
    23881 cgtcgccgct tgtcagcgac gtcatgttca cgtctgacta agccacgatt ttccattctt
    23941 gccaacattt ctgctagcgt tgctttggtg ctgactgctg cttctatcag tgccacctgt
    24001 tcaataccag gtttatcagc aatggcacgc atcaccgcgt actgtggttt ggtcaggtca
    24061 ggtaactcgt gctgccagcg agccgtgtgc tgctgaaaaa gctgtcgtaa ctgatggaac
    24121 gctttatttc gtaacgccat gtaaactccg ggctaattct ctgtcgctat catagcggtt
    24181 ttttagtgac gaagaagatg ttgttacttt tcaatccttg ccgctgtgga aatgaaggag
    24241 tatgttaata ataacgttcg tatacgaata attaagagaa taagcaatga aactgatcgt
    24301 cgggatgaca ggggctaccg gtgcgcctct tggtgtggca ttactgcaag cgctgcgtga
    24361 gatgccgaat gtcgagactc atctggtgat gtcgaagtgg gcgaaaacca ccattgaact
    24421 ggaaacgcct tacagcgctc gcgatgttgc tgccctcgca gacttcagcc ataacccggc
    24481 ggatcaggcg gcgatcatct catccggttc ttttcgtacc gacggcatga tcgttattcc
    24541 gtgcagtatg aaaacgctcg ccggtatccg cgctggttac gccgatggcc tggtagggcg
    24601 cgcggcggac gtcgtgctca aagaaggccg caaactggtg ctggtgccgc gtgaaatgcc
    24661 gcttagcacc atccatctcg aaaatatgct cgcactttca cgcatgggcg tggcgatggt
    24721 gccgccgatg cctgcctttt ataaccatcc cgaaacggta gatgacattg tccaccatgt
    24781 ggtagcccgc gtgctggatc aatttggcct cgaacatccc cacgccaggc gctggcaagg
    24841 attaccgcag gcccggaatt tttctcagga gaatgaataa tggcatttga tgatttacgc
    24901 agctttttac aggcgcttga tgaccacggc cagttactga aaatcagcga agaagtgaac
    24961 gctgagccgg atctggcagc agcagctaac gccaccgggc gtatcggcga cggcgcgccc
    25021 gcgctgtggt ttgataatat tcgcggcttt accgatgccc gcgtggcgat gaataccatc
    25081 ggttcctggc agaaccacgc gatttccctt ggcctgccac caaacacccc ggtcaaaaag
    25141 cagattgatg agtttatccg ccgctgggat aacttcccga ttgccccgga gcgccgcgcc
    25201 aatccagcct gggcgcagaa caccgtcgat ggcgaagaga tcaacctgtt cgatattctg
    25261 ccgctgtttc gcctgaatga tggcgatggc ggtttctatc tcgacaaagc gtgcgtggtt
    25321 tcccgcgatc cgctcgaccc ggataacttc ggcaagcaga acgtcggcat ctaccgcatg
    25381 gaagtgaagg gcaagcgtaa gctcggcctg caaccggtgc cgatgcacga tatcgccctg
    25441 catctgcata aagcagaaga gcgcggtgaa gatctgccga ttgcgatcac gctcggtaac
    25501 gatccgatca tcacgctgat gggggccacg ccgctgaaat acgatcagtc tgagtatgaa
    25561 atggcaggcg cgctgcgtga aagcccgtac ccgatcgcca ccgctccgct gaccggtttt
    25621 gatgtgccgt ggggttcaga agtgatcctc gaaggggtta tcgaaagccg taaacgcgaa
    25681 atcgaagggc cgttcggtga gtttaccggg cactactccg gtgggcgcaa catgaccgtg
    25741 gtgcgcatcg ataaagtctc ttatcgcagc aaaccgattt tcgaatcact ctatctcggt
    25801 atgccgtgga ccgaaatcga ctacctatga ctaggtaatt tattcaagat aaccgggaag
    25861 agattaaaaa agacgggaat agattggaat caacaagtta ggaactcaag gttttcaaga
    25921 taaagcgagt ccaaaaatgt taaacggttc gatatcaata aaacattcaa gataactcac
    25981 aggttcttac aatagccttt aaagaccgtt caaaaacgat tcaggtgttt attatagcgc
    26041 tgatgggtgc aagcgccaaa accaatgtaa ttttagcaca gctaggcact tgcactgggt
    26101 tatgcgctgg ctgctttctg aggtagattt aagtgatcgc ccttagcttc ggacaacgcg
    26161 taaatctctc taaccttttc gtctggcaaa ttaccaaggc ttaacgctat ggctctacga
    26221 actccaacta gagcgtgcaa ctcttggatg tgctcatcca gaagaatgag taattgctcc
    26281 gctccttttc tcccaagaac atcagctact tgttgttttt cttgtggttt aagaaatgac
    26341 aagaacatta gaatttgagc atcaacatta tccgcattct cttttttggg ttctttggaa
    26401 tcttttagtt caacatcacc ctctcccgtc atgagccatt ccaaggtaac attttcgctt
    26461 tctgctatct tttttaactg atgtgggcgt ggtatcggtg cgtaatcccg cctgttgtaa
    26521 taatttttta aagtgccttc attgatcccc catgcccgag cagccgcatt tcttgaaggg
    26581 tatcgttcta ctaacctaaa gattctttcg ataatcggtt cttttccatc ggcaggaaaa
    26641 gaaccttggg gtgcttttct cattcttggt tcctttctat ctaattcatt gataaataat
    26701 aaaattaaaa aataaaccat aaaaacacca aagaaccctt tacagggaat ctttggtatc
    26761 ttaatcttta tccatacgga tatccgcgcg ggtaattttg taggttaaag gaaagatgag
    26821 acaaagagaa gatagtgacc gcgattggcc ttcgctgaag attcagctgg cgctcaaaga
    26881 taacgggtgg tcttccttcc gggagctttc actccatgct ggttatgctc aggattcact
    26941 gaaagcggta gttcgcacac caaacaaaaa atacgaacgc gtaatttctg acgctatagg
    27001 tgtaccgcca gaaatcatct ggcccagccg gtatcgtaac gatggattta tcaggaaggc
    27061 gtcgtgatat gcagtcgtac gttaccgtaa atgacctgct cggcgtacca ggaatgccag
    27121 ccaccacaaa aggcatccga caggcgttac agcgtttttc tcgtgatctt ggtgacgtta
    27181 gtcgccgtcg ggaaggcaca aaagccattg agtaccacat tgattgtctg cctgaaataa
    27241 cacgaaaggc actgcgtgaa cgctatgtgg aacagctggt ggctacagag aacaatgttt
    27301 ctgaagtgaa agctgttacc agaaaaacac gcaatcctga cgctgtccag gcaatcgaag
    27361 catatcgcgg ttctccacaa ctgatggaag aacgcctgaa tgcgctgacc gaaaaccagc
    27421 gctgggtatc tgaagcaaga gctgcgctgg tggtggaagt gctgaagctg gaaagcgccg
    27481 gtaaccccgg gcgactgaaa gccattaact ttcttgttga aaaagcccgt aaaggtgagc
    27541 tgccggagcg cctgcaacag gccgcagtta acgccaatgc aaaacgtggc gctaatcgta
    27601 ctatcagccg cgatccactt taccagtggg ttctgaaata caaccagtcg cagaacgccg
    27661 ccgaacggct gctgttactg gcaccgggaa aacgcgacga aataaaaccg gaagaaatca
    27721 gctggctgcc tgaatttctg gcgcagtatc gccaggtgaa cggcagaccc atgtcagaag
    27781 cctacgagga ttttgtcgca gaatggcagc gtcgccatgc ggacgaaccg tacatgctgg
    27841 aggtgatgcc ttcctatgat gttgtccggt atgccatgaa aaaactgccg gaagtggtga
    27901 aacaaaaagg gcgcgttacc ggcagtgaat accgtcagct tgaaggattc acccgccgcg
    27961 actggacagc tatgccggtg aattatgtct ggattggtga cggtcacggt atgaagctga
    28021 aatgtgcgca tccgatccac gggcgtccat tctcaccgga agtcacgttt gtgatcgatg
    28081 gtggaacacg gtttgttgtt ggctggagtc ttgaccttgc cgaaaacgtg ttcgccgttg
    28141 ccggtgcgat acagcacggc attcgcaacc acggaaaacc gttcctgtat tactcggata
    28201 acggttccgg tgaaacggct gacatgctgg acaaagaggt cgtgggtatt ctgccacgcc
    28261 tggggattaa acaccctacc ggtattgccg gtaatccaca gggacgcggc attattgaac
    28321 gcctgaaccg gacactgccg atgcgtatag ccaggaggta tcgcacctat tttggcaagg
    28381 gtgctgaccg ggaatcgttg cgtgtactta accgtgatct gcgttcagcg tttaacgccc
    28441 tgcaacagga caagcctctg aacgaccgcc agaaagcggc aatgcgcgag ctgccatcgt
    28501 gggcggagct tattgaggct attcgtgaag gcgtggaatg gtacaacaac cgcccgcatt
    28561 ctgagctgcc gatgaaaccg aacggacggc attacagccc gacagagttc agaaaaaaac
    28621 gccaggcaga agaagacacc gaaattgagt ggctgagtga tcttgagctg cgcgacatgt
    28681 tccgcccaat ggtggaacga ccggtaagac gctgcgaaat tcagtggctg aacaatattt
    28741 actacgcgcc ggagttgcgt gacgaacacg gtcgcaaagt gcttatcagt tatgacattc
    28801 atgacgccga gcgaattacg gtacgtcgca aggacggcag ctttatctgt gaggcgatat
    28861 ggaacggcaa taaacgcgca gcctttgctg tcagtgctga atatcacaaa caacagcagc
    28921 gtattaaagg aatgcgcaaa cgcgctgagg aaaaaattcg tgatgctgaa gatgaaggta
    28981 ttcagattct ggaacacaaa caggctgaac cctggctgag taatgtttat cgcccggttg
    29041 gtaatgtggt ggcagttcag caaccggaat atgaagaaga gcatgacgaa gaatttgaac
    29101 gcgatttccg gctggggatg caaaaacttt ttgccatgca ggaagaagat gatccgctgg
    29161 cctgaataaa aaaacagtcc gcgggaacgg actgttaaat aaaggaaaca aagctgattt
    29221 gaaataaccg aggaaatact atgactgata ttgatga
//
LOCUS       DAJTQY010000047        29236 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.047, whole genome shotgun sequence.
ACCESSION   DAJTQY010000047 DAJTQY010000000
VERSION     DAJTQY010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29236
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.047"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            <1..218
                     /locus_tag="OO349_002140"
     CDS             <1..218
                     /locus_tag="OO349_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5867200.1"
                     /translation="DTAMNFIRQGLGIALQPELTLKSIAGELCSVPHEPTFYRQISLL
                     TKEKPVEGSPLFLLQMCMEQLVAIGKI"
     gene            complement(260..1336)
                     /gene="glpQ"
                     /locus_tag="OO349_002141"
     CDS             complement(260..1336)
                     /gene="glpQ"
                     /locus_tag="OO349_002141"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCR5867201.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMELNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTPDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(1341..2699)
                     /gene="glpT"
                     /locus_tag="OO349_002142"
     CDS             complement(1341..2699)
                     /gene="glpT"
                     /locus_tag="OO349_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HCR5867202.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            2972..4600
                     /gene="glpA"
                     /locus_tag="OO349_002143"
     CDS             2972..4600
                     /gene="glpA"
                     /locus_tag="OO349_002143"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HCR5867203.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            4590..5849
                     /gene="glpB"
                     /locus_tag="OO349_002144"
     CDS             4590..5849
                     /gene="glpB"
                     /locus_tag="OO349_002144"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HCR5867204.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVADIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            5846..7036
                     /gene="glpC"
                     /locus_tag="OO349_002145"
     CDS             5846..7036
                     /gene="glpC"
                     /locus_tag="OO349_002145"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HCR5867205.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRNIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            7229..8179
                     /locus_tag="OO349_002146"
     CDS             7229..8179
                     /locus_tag="OO349_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HCR5867206.1"
                     /translation="MTESTTSSPHDAVFKTFIFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRPMRYATAA
                     MQSHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDHDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSPLQKERLMTIAERLRQEGHQIGWQEGKLVGLQQGKLEGLQ
                     EGMHEQAIKIALRMLEQGIDRDQVLAATQLSEADLAANNH"
     gene            complement(8220..8690)
                     /locus_tag="OO349_002147"
     CDS             complement(8220..8690)
                     /locus_tag="OO349_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001342598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HCR5867207.1"
                     /translation="MSNPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESK
                     TALDNLDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAA
                     GFLAVAPDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(8745..9947)
                     /locus_tag="OO349_002148"
     CDS             complement(8745..9947)
                     /locus_tag="OO349_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HCR5867208.1"
                     /translation="MLKVEMLSTGDEVLYGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVIPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            complement(10034..10576)
                     /locus_tag="OO349_002149"
     CDS             complement(10034..10576)
                     /locus_tag="OO349_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5867209.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGLYVGVNASF"
     gene            10855..11280
                     /gene="nudI"
                     /locus_tag="OO349_002150"
     CDS             10855..11280
                     /gene="nudI"
                     /locus_tag="OO349_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HCR5867210.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            complement(11319..11921)
                     /locus_tag="OO349_002151"
     CDS             complement(11319..11921)
                     /locus_tag="OO349_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCR5867211.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDAIFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            12229..13368
                     /gene="arnB"
                     /locus_tag="OO349_002152"
     CDS             12229..13368
                     /gene="arnB"
                     /locus_tag="OO349_002152"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HCR5867212.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAESGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRTAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            13372..14340
                     /gene="arnC"
                     /locus_tag="OO349_002153"
     CDS             13372..14340
                     /gene="arnC"
                     /locus_tag="OO349_002153"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HCR5867213.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILINRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            14340..16322
                     /gene="arnA"
                     /locus_tag="OO349_002154"
     CDS             14340..16322
                     /gene="arnA"
                     /locus_tag="OO349_002154"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HCR5867214.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VAHLAAERDIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEIFQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQMLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFLPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSCYYGKGYQDVEHRKPSIR
                     NAHRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            16319..17209
                     /locus_tag="OO349_002155"
     CDS             16319..17209
                     /locus_tag="OO349_002155"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HCR5867215.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAIPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            17209..18861
                     /gene="arnT"
                     /locus_tag="OO349_002156"
     CDS             17209..18861
                     /gene="arnT"
                     /locus_tag="OO349_002156"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HCR5867216.1"
                     /translation="MKSVRYLIGIFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLLAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVISPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIVRQERLVLIQYRPK"
     gene            18858..19193
                     /gene="arnE"
                     /locus_tag="OO349_002157"
     CDS             18858..19193
                     /gene="arnE"
                     /locus_tag="OO349_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HCR5867217.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMMLWLLVSQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            19193..19579
                     /gene="arnF"
                     /locus_tag="OO349_002158"
     CDS             19193..19579
                     /gene="arnF"
                     /locus_tag="OO349_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HCR5867218.1"
                     /translation="MGLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(19573..19845)
                     /gene="pmrD"
                     /locus_tag="OO349_002159"
     CDS             complement(19573..19845)
                     /gene="pmrD"
                     /locus_tag="OO349_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HCR5867219.1"
                     /translation="MQWNGWHKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKV
                     GDLLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(19950..21305)
                     /gene="menE"
                     /locus_tag="OO349_002160"
     CDS             complement(19950..21305)
                     /gene="menE"
                     /locus_tag="OO349_002160"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HCR5867220.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQLVEGVHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGLPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVSVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(21302..22264)
                     /gene="menC"
                     /locus_tag="OO349_002161"
     CDS             complement(21302..22264)
                     /gene="menC"
                     /locus_tag="OO349_002161"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HCR5867221.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQENWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELTDTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDADVQEQLL"
     gene            complement(22264..23121)
                     /gene="menB"
                     /locus_tag="OO349_002162"
     CDS             complement(22264..23121)
                     /gene="menB"
                     /locus_tag="OO349_002162"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HCR5867222.1"
                     /translation="MIYPDEAMPYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(23136..23894)
                     /gene="menH"
                     /locus_tag="OO349_002163"
     CDS             complement(23136..23894)
                     /gene="menH"
                     /locus_tag="OO349_002163"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HCR5867223.1"
                     /translation="MILHAQAKHGKLGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLLGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(23891..25561)
                     /gene="menD"
                     /locus_tag="OO349_002164"
     CDS             complement(23891..25561)
                     /gene="menD"
                     /locus_tag="OO349_002164"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HCR5867224.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HHPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(25650..26945)
                     /gene="menF"
                     /locus_tag="OO349_002165"
     CDS             complement(25650..26945)
                     /gene="menF"
                     /locus_tag="OO349_002165"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HCR5867225.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(27024..27329)
                     /gene="elaB"
                     /locus_tag="OO349_002166"
     CDS             complement(27024..27329)
                     /gene="elaB"
                     /locus_tag="OO349_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HCR5867226.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKAHAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(27384..27845)
                     /locus_tag="OO349_002167"
     CDS             complement(27384..27845)
                     /locus_tag="OO349_002167"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5867227.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            27910..28827
                     /gene="rbn"
                     /locus_tag="OO349_002168"
     CDS             27910..28827
                     /gene="rbn"
                     /locus_tag="OO349_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HCR5867228.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            29018..29206
                     /locus_tag="OO349_002169"
                     /pseudo
     CDS             29018..29206
                     /locus_tag="OO349_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311184.2"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
BASE COUNT         7086 a         7857 c         7300 g         6993 t
ORIGIN      
        1 cggatacagc gatgaatttt attcgacagg gattaggcat cgccttacaa ccagagttaa
       61 cgctgaaaag cattgcaggt gaattgtgtt ccgttcctca cgaaccaacc ttctatcgac
      121 agatttcgtt gctgactaaa gaaaagccgg tagaaggcag tccactgttt ttactacaaa
      181 tgtgcatgga acaattagtg gcgattggaa aaatttgata tgagacagga tggcgcatga
      241 acgccatcct gcagtaacat tactctttat taagaaattt tactgcctta tcagggaaat
      301 cagtaaacag cccatttaca cccgctttgt tatacagagc atcatataac tgattcacat
      361 caggagtgta ttcaggcagt ttatctgacc gcacggtata aggatgcact accagtttat
      421 tctgctgggc atcttgcacc atgccagtga gtttgatatt acccggctgc gatgtctcct
      481 caatcaacat atggtaatcc ggaccaatac catctgcata ttccgccacc tgtttcatgg
      541 ctcccggctt aaacatccag tcgtagttgt aattaaccca gcttccatcc ggctgtttct
      601 gctgcgtttc attccagtcg gtataggcaa tcagctgtac cagattgagc tccatgccca
      661 ttttgggttc cagctcattc ttaatacgct tcagctcatc agcatcaaaa cattgcaaat
      721 aaactttatc gtctttaccg gtgtaaccat atttcttcag cacttccagc gtttttgccg
      781 caatatcctt cccttcctga tgatggaacc acggcgcttt gatttctgga tagataccga
      841 tatttttccc ggtagagtga tttaacccct gaacaaattc aatctcttct tcaaaggtgt
      901 gcacccggaa gtcggactta cccattggga aacgccccgg ataagtctgt acttttttac
      961 cgttttcaat atcgaaacct tcggtaaatt tcagcgactt aatttcatcc agcgtgaaat
     1021 ctatcgcgta gtaacgaccg tctttgcgcg cccgatccgg gaaacgatcg gcaacatcag
     1081 taacacgatc gagataatgg tcatgcagaa caaccagatg gtcgtctttg gtcatcacca
     1141 aatcctgttc cagataatcc gctccctgcg catacgccat cgcttttgct ggcagcgtat
     1201 gctccggcaa atatccactg gcaccgcgat gggcgatgac tattttttcg ttgctgtccg
     1261 ccgccattgc actgcttccc atgactatag tgctcatcat gatcgccatg ctaaggtttt
     1321 tcagcttcaa tttcatgcca ttagcctccg ttgcgttttt gcagtaattg ttcatggcga
     1381 cgtttttcgc caatcatcac aacaatcaac aagataaccg ccagaatgct gccgccaatc
     1441 attaccataa agccgccatc ccagccgaag aagtccacgg tgtagccaac aatcgcgctc
     1501 gccgccaccg aaccgcccag gtaaccaaac agcccggtaa agcccgctgc cgtacctgcc
     1561 gcttttttcg gtgccagttc cagcgcatgc agaccgatca gcatcacagg accgtagatc
     1621 aggaagccga taacaatcat acaaatcata tcgacggttg ggttacctgc cgggttcatc
     1681 cagtaaacga tagtcgcgat ggtcaccagt gtcataaaga agacgccggt tgccccacgg
     1741 ttaccacgga agactttgtc cgacatccag ccgcacagca gggtgcccgg aatacccgca
     1801 tactcataga agaagtaggc ccaggaggat ttatccagcg cgaaatgctt aacctctttc
     1861 agataagtcg gtgaccagtc gaggatgccg taacgcagca gataaacgaa cacgttggcg
     1921 atggcgatgt accacagcag tttgttcggc agcacgtact gcatgaagat ttgcttcgcg
     1981 gtcagctcct gttccgcttt ttcgttatag tcgtccggat aatcattttt gtactcttcg
     2041 atcggcggca agccacagga ttgcggggta tcgcgcatca tcgcaaaggc gaataatgcc
     2101 accagaatgg cgcagaaagc aggcatatag agcgccgcat gccagtcatt gaaccaggcc
     2161 atccccagca ggaacagcag cggcggaata ccaccaccga cgttgtgcgc acagttccac
     2221 actgacacaa tgccgccacg ttctttctgc gaccaccagt gcaccatagt acgaccacac
     2281 ggcggccacc ccatcccctg gaaccaaccg cagaggaaca acagtacaaa catcaccgca
     2341 atgctcgacg tcgcccatgg cacaaagccc ataaacaaca tcactgccgc cgccagaatc
     2401 aaacctgcgg gcaggaaaac gcgcggattc gagcgatccg ataccgaacc catgatgaat
     2461 ttcgaaaatc cataagcaat cgagatcccc gaaagggcaa aacctaaatc accgcgtgag
     2521 aatccctgct caaccagata aggcatagca agcgcaaagt tcttacgaac caaatagtaa
     2581 gccgcatagc caaagaatat ccccaggaaa atttgccagc gcaatcgacg ataagtcgga
     2641 tcgatctccg cggcaggtaa gcgcgctttg tgtggcgctg gtttaaaaat actcaacatt
     2701 gatagcctcc gtggcccgtg gtcttattta tgattaacag cctgattcag tgagagaacc
     2761 tgccgtttct tgagttgccg cgatgttaag aaaacattca taaattaaat gtgaattgcc
     2821 gcacacatta ttaaataaga tttacaaaat gttcaaaatg acgcatgaaa tcacgtttta
     2881 ctttcgaatc atgagcgaat atgcgcgaaa tcaaacaatt catgttttta ctatggctaa
     2941 atggtaaaaa acgaacttca gagggataac aatgaaaact cgcgactcgc aatcaagtga
     3001 cgtgattatc attggcggcg gcgcaacggg agccgggatt gcccgcgact gtgccctgcg
     3061 cgggctgcgc gtgattttgg ttgagcgcca cgacatcgca accggtgcca ccgggcgtaa
     3121 ccacggcctg ctgcacagcg gtgcgcgcta tgcagtaacc gatgcggaat cggcccgcga
     3181 atgcattagt gaaaaccaga tcctgaaacg cattgcacgt cactgcgttg aaccaaccaa
     3241 cggcctgttt atcaccctgc cggaagatga cctctcattc caggccactt ttattcgcgc
     3301 ctgcgaagaa gcagggatca gcgcagaagc tatagacccg cagcaagcgc gcattatcga
     3361 acctgccgtt aacccggcac tgattggcgc ggtgaaagtt ccggatggca ccgttgatcc
     3421 atttcgtctg accgcagcaa acatgctgga tgccaaagaa cacggtgccg ttatccttac
     3481 cgctcatgaa gtcacggggc tgattcgtga aggcgcgacg gtgtgcggtg ttcgtgtacg
     3541 taaccatctc accggcgaaa ctcaggccct tcatgcacct gtcgtggtta atgccgctgg
     3601 gatctggggg caacacattg ccgaatatgc cgatctgcgc attcgcatgt tcccggcgaa
     3661 aggatcgctg ctgatcatgg atcaccgcat taaccagcat gtgatcaacc gctgccgtaa
     3721 accttccgac gccgatattc tggtgcctgg cgataccatt tcgctgattg gtaccacctc
     3781 tttacgtatt gattacaacg agattgacga taatcgagtg acggcagaag aggttgatat
     3841 tctgctgcgt gaaggggaaa aactggcccc cgtgatggcg aaaacgcgca ttttgcgggc
     3901 ctattctggc gtgcgcccgc tggttgccag cgatgacgac ccgagcggac gtaacgtcag
     3961 ccgtggcatc gtgctgctcg accatgctga acgcgatggt ctggacggat ttatcaccat
     4021 caccggtggc aaactgatga cctatcggct gatggctgaa tgggctaccg acgcggtatg
     4081 ccgcaaactg ggcaacacgc gcccctgtac gactgccgat ctggcactgc ctggttcaca
     4141 agaacccgct gaagttacct tgcgtaaagt catctccctg cctgccccgc tgcgcggttc
     4201 tgcggtttat cgtcatggcg atcgcacgcc tgcctggctg agcgaaggcc gtctacaccg
     4261 tagcctggta tgtgagtgcg aagcggtaac tgcgggtgaa gtgcagtacg cggtagaaaa
     4321 tttaaacgtt aatagcctgc tggatttacg ccgtcgtacc cgtgtgggga tgggcacctg
     4381 ccagggcgaa ctctgcgctt gccgcgctgc cggactgctg caacggttta acgtcacgac
     4441 gtccgcgcaa tctatcgagc aactttccac cttccttaac gaacgctgga aaggcgtgca
     4501 acccatcgcc tggggagacg cactgcgcga aagcgaattt acccgctggg tttatcaggg
     4561 actgtgtggt ctggagaagg agcagaaaga tgcgctttga tactgtcatt atgggcggtg
     4621 gcctcgccgg attactctgt ggcctgcaac tgcaaaaaca cggcctgcgc tgtgccattg
     4681 tcacgcgtgg tcaaagcgcg ttgcatttct catccggttc gctggatttg ctgagccatc
     4741 tgccagatgg tcaacccgtg gcagacattc acagtggact ggaatctttg cgtcagcagg
     4801 cacctgcaca tccttactct cttctcgggc cacaacgcgt gctcgatctc gcttgccagg
     4861 cgcaggcatt aatcgctgaa agcggtgcgc aattgcaggg cagcgtagaa ctggctcacc
     4921 agcggattac gccgctcggc actttgcgtg caacctggtt aagttcgcca gaagttcccg
     4981 tctggccgct acccgcgaag aagatatgtg tagtggggat aagcgggctg atggatttcc
     5041 aggcgcatct tgcggcggct tcgttgcgcg aactggatct ttcggttgaa accgcagaaa
     5101 tagagctgcc ggaactggat gtcctgcgca ataacgccac cgaatttcgc gcggtgaata
     5161 tcgcccgttt ccttgataat gaagaaaact ggccgctgtt acttgatgcg cttattcctg
     5221 tcgccaatac ctgcgaaatg atcctgatgc ccgcctgctt cggcctggcc gatgacaaac
     5281 tgtggcgttg gttgaatgaa aaactacctt gttcactgat gcttttgcca acgctgccgc
     5341 cttccgtgct gggcattcgt ctgcaaaacc agttacagcg ccagtttgtg cgccaaggtg
     5401 gagtgtggat gccgggcgat gaagtgaaaa aagtgacctg taaaaatggc gtagtgaatg
     5461 aaatctggac ccgcaatcac gccgatattc cgctacgtcc acgtttcgcg gttctcgcca
     5521 gcggcagttt ctttagtggc ggactggtag cggaacgtaa cggcattcgc gagccgattc
     5581 tcggccttga tgtgctacaa accgccacgc ggggtgaatg gtataagggc gatttttttg
     5641 cgccgcaacc gtggcagcag ttcggtgtaa ccactgatga gacgctacgc ccgtcgcagg
     5701 cagggcaaac cattgagaac ctgtttgcca tcggttcggt gctgggcgga tttgatccca
     5761 tcgcccaggg atgcggcggc ggtgtttgtg ccgtcagtgc tttacatgcc gctcaacaga
     5821 ttgcccaacg cgcaggaggc caacaatgaa cgacaccagc ttcgaaaact gcattaagtg
     5881 caccgtctgc accaccgcct gcccggtgag ccgggtgaat cccggttatc cagggccaaa
     5941 acaagccggg ccggatggcg agcgtctgcg tttgaaagat ggcgcactgt atgacgaggc
     6001 gctgaaatat tgcatcaact gcaaacgttg tgaagtcgcc tgcccgtccg atgtgaagat
     6061 tggcgatatt atccagcgcg cgcgggcgaa atatgacacc acgcgcccgt cgctgcgtaa
     6121 ttttgtgttg agtcataccg acctgatggg tagcgtttcc acgccgttcg caccaatcgt
     6181 caacaccgct acctcgctga aaccggtgcg gcagctgctt gatgcggcgt taaaaatcga
     6241 tcatcgtcgc acgctaccga aatactcctt cggcacgttc cgtcgctggt atcgcagcat
     6301 agcggctcag caagcacaat ataaagacca ggtcgctttc tttcacggct gcttcgttaa
     6361 ctacaaccat ccgcagttag gtaaagattt aattaaagtg ctcaacgcaa tgggtaccgg
     6421 tgtacaactg ctcagcaaag aaaaatgctg cggcgtaccg ctaatcgcca acggctttac
     6481 cgataaagca cgcaaacagg caattacgaa tgtagagtcg atccgcgaag ctgtgggagt
     6541 aaaaggcatt ccggtgattg ccacctcctc aacctgtaca tttgccctgc gcgacgaata
     6601 cccggaagtg ctgaatgtcg acaacaaagg cttgcgcgat catatcgaac tggcaacccg
     6661 ctggctgtgg cgcaagctgg acgaaggcaa aacgttaccg ctgaaaccgc tgccgctgaa
     6721 agtggtttat cacactccgt gccatatgga aaaaatgggc tggacgctct acaccctgga
     6781 gctgttgcgt aacatcccgg ggcttgagtt aacggtgctg gattcccagt gctgcggtat
     6841 tgcgggtact tacggtttca aaaaagagaa ctaccccacc tcacaagcca tcggcgcacc
     6901 actgttccgc cagatagaag aaagcggcgc agatctggtg gtcaccgact gcgaaacctg
     6961 taaatggcag attgagatgt ccacaagtct tcgctgcgaa catccgatta cgctactggc
     7021 ccaggcgctg gcttaaactc ccttctgatg cccggtaagc atgtggttac cgggcatttt
     7081 tgcgtacacg attccgtgcc caatgtatgc gttgcaacgc agtgaaaatt cctctgaaaa
     7141 cgtctcgcaa aggctgaaac tggcagatgt caaaggcctg ggataaccgt aatgtcgcgt
     7201 catcataaat atcaggtgac ggacaaccat gaccgaatca acaacctcct ccccgcatga
     7261 tgcggtattt aaaaccttta tattcacacc cgaaaccgca cgggattttc tcgaaataca
     7321 tttaccagaa ccactgcgca agctttgcaa cctgcaaacc ttacgcctgg aacccactag
     7381 ttttattgaa aaaagtttac gcgcttacta ctcggatgtt ttgtggtccg tggaaaccag
     7441 cgacggtgac ggctatatct actgcgtgat tgaacatcaa agctctgcag aaaagaatat
     7501 ggcttttcgg ccaatgcgct atgccactgc cgccatgcag agtcacctag ataaaggcta
     7561 tgacagagtt ccgctggtgg tgccattgct gttttatcat ggcgaaacat cgccctaccc
     7621 gtactcactt aactggctgg atgagtttga cgatccgcaa cttgcccggc agttgtacac
     7681 cgaagctttt ccgttggtgg atatcaccat cgtacctgac gatgagatca tgcaacatcg
     7741 gcgtatagct ctgctggaac tgattcaaaa gcatattcgc gaccacgatt taatcggcat
     7801 ggtcgacagg atcaccacgc ttttggttag aggcttcact aatgacagcc agctacaaac
     7861 actgtttaat tatctgctgc aatgcggcga tacctcccgt ttcacccgtt ttattcagga
     7921 gattgccgaa cgttcaccac tacaaaagga gagattaatg actattgctg aacggctacg
     7981 gcaggaagga catcaaattg gctggcaaga aggtaaatta gtaggtttgc agcaaggtaa
     8041 attagaaggt ttgcaggaag gtatgcatga acaagccatt aaaattgctt tgcgcatgct
     8101 ggaacagggc attgatcgtg accaagtgct cgcggccacc cagctaagcg aagccgatct
     8161 ggcagcgaat aaccactaat taacacaggc ccacagccga tccccatggg cctttgatat
     8221 caataactac cttttatgcg tggcccattt ttgcctgatt taaacatcgc cagtcgttga
     8281 tccagggcat cgctgtagag catcgtgtca acgccaacag cgacaaagtt cgctccccac
     8341 gccaggcatt gctgcgccat atcaggagcc acagccagaa aaccagccgc tttacccgca
     8401 gcacggatcc gccgaatgct ggtttcaata attcgctgca cttccgggtg cccggcgtta
     8461 tccgggtagc ccaacgacgc agaaagatcc gctggtccaa taaacacgcc atcaatccct
     8521 tcgacgtcga ggatctcgtc caggttatcc agtgccgttt tactttccac ctgcactaac
     8581 agacaaagcg aatcgttgac ttgcgccatg taattctcaa tgcgtcccca gcgcgcagcc
     8641 cgtgccacac tggccccaac accacgctca ccgtagggag ggttggacaa attttgccgg
     8701 agggggaatg tgccggtgca gtaaaccggc acagagtggc gaaatcagac ggataaagtc
     8761 atggactcaa caacctcaat ccagccatgc tcactagcga tgtcctggcc gtttaaccaa
     8821 cggcgtagca tattcagtgc catcattgcg cacacttcct gacggatagc caggctgtag
     8881 cgtgtagtgc tgaaacgcac acgcagagcg aaagtgccgt ctggcgtggc tagcgcaaag
     8941 ttgagatgct cgttctcgaa acccgaaaca gccagtgcca gcccggcaaa atggttggcc
     9001 cgccgttctg taatccagtg cgcagtttgc gccagggttt cttcctgtga aggaatcact
     9061 tcacacgcca gcaatggagc acctgcgcga gaaagttgca aagccaataa accaccggtg
     9121 aattgctcgc tcaacgtcag gctgaactgg cggttttgca attcgcgact gatctgcgcg
     9181 ggcagtcctt cggtgccttc gaaaatcacg ctctgtccgg caacacgttt aacgtccagc
     9241 cacagttttt ccatcgcctg ttgctcgctt gccggtccgg tgagtttcag ttcgatgatc
     9301 ggcattgagg agcgatagcc cattgttacg cccggcggca gttgtagagt gtccaggctt
     9361 tgtgccagat cgctttccga acgaccaaag gtagttaaac gcagacaaac cggcggctgt
     9421 ggtaaagaaa aacgctcgcg caggcgcggc aggatttcgt gctcgaccat caccttaaat
     9481 tctgacggta cgccgggagt aaagaacatc aggcaacgat taagctgcac ggcaaaacca
     9541 caggcggtgc ctaccgggtt attgataaat tcagcactgg caggcagctc cgcttgttta
     9601 cggttgctcg gtgccattac tcgtccacgt tcgtggaaat agcgttccat ctctttgagc
     9661 caggcttcat gcagcaccag gccttcacct tttgctgtcg cagcggcgag tgcgcttaaa
     9721 tcatcgctgg tcggtcccag cccgccgtta acgatcagca catcggcgtg ctgactacgt
     9781 tcgcgaagaa tggtgactaa gtcatcaagg ttatccccca ccgtattgcg gcgagataat
     9841 ggcagcccct gatgaaagaa aaaatcggcc agccaggcag cgttagtgtc aacgatttgc
     9901 ccgtataaca cttcatcccc ggtggatagc atttccactt ttaacatggt tatctcctgc
     9961 ttttgttatc gaatcactat aacgcaaagg gagataaggc gtagtaagaa actgaatgcc
    10021 gcgccggaag ggatcagaaa ctggcgttaa ccccaacata caggccgtca gccacagcgt
    10081 tgtcgcggtt accgtcttta cccgacagat tcaggtagcg ataacccgcc tcaatactga
    10141 ctggacgcat aatggtgtaa cgcgcgccag cattcgcttc ctcataactt tgaataccgc
    10201 tggagagcga atccggagag tagtaatact cgccaaacaa acggaagctg ttgccaattt
    10261 tccactgcaa accacctcct accgctgcgg cataaccttc atcgccgtaa ttcgggttgg
    10321 tgtacacgcc ttttccgcca acggtcgcca ttaacggccc gagaggcaga ttcaacccca
    10381 gccccacgcc cgcgacgtca ccgtcgtcgt cgttatgtgt ccagttaccg cttaaagcca
    10441 ggcccgtcga ttcagtccca aaaccgacac caatattggt gtattcttta cccgcctggc
    10501 cgctgatgct cattgcattg accgatgcag aaaccaacag cattccggcc agacctgcta
    10561 gcgcaatttt tttcattatc ccctttccca caagcaatta atcaaaattc ccaaagctgc
    10621 gggattgtac tgcctggcac agaggattca atgagcgaaa aaagggatct agtcacttat
    10681 tgtaactaaa tacttcttaa ataattaaac gccgaaaaaa taaacacact ttaccgatac
    10741 acacaaaaat aataataagt gcagttatca ttgcaaaaga gattactgtc actttcacat
    10801 atttctgagt taccgtattc tcatggtttc tttttattta taaggaatac ccctgtgcga
    10861 caacggacta ttgtatgccc tttgattcaa aatgatggtg cttatttgct gtgcaaaatg
    10921 gccgacgatc gcggcgtttt ccccggtcaa tgggcgcttt cgggtggcgg cgtggagtct
    10981 ggcgaacgaa ttgaagaggc actacgccgc gaaattcgcg aagaactggg agaacagctg
    11041 cttttgacag aaatcacgcc gtggaccttc agcgatgata ttcgcaccaa gacatatgca
    11101 gatggtcgca aggaagagat ttatatgatt tacctgattt ttgactgcgt ttctgccaac
    11161 cgagaagtga aaataaacga agagtttcag gactacgcgt gggtaaaacc tgaagatctg
    11221 gtgcattatg atttaaatgt tgccacccga aaaacgttac gtttgaaagg tcttctttaa
    11281 caatgatgac agccatcact ggctgtcaga ttgaaaattt agtggttaac gaattcccca
    11341 tccagataaa ctttgccttt ttccacatgc atgactaaac catccagata atcaggttta
    11401 aatatcgcat cacgcttatt tttagcaata tatgtcaggc aatgattatg ggtgaaaata
    11461 acgatatttt tatcaggcgc tttgctttgt aagtccttaa ttgcactata aatctcatta
    11521 ccgcactgaa gaagtcgttt atctaccgtc agctttttac ccgcggaaaa ccaggtcgcc
    11581 gactgaatgg tccggacggt attactggaa taaagatcga aatcagggat gtcagcgcta
    11641 aaagcgttgc ccagttcacg ggcatcctgg gtacctttaa ccgtaatacc tgttttatct
    11701 gacaagcatt gattggttga acgatcgcaa cgttcagcat ggcgaaacaa aacgacaacc
    11761 gggtgctgct gtgccagtct ggccaatgtt ttattgtcga tacgtggcaa accattgcta
    11821 ctccaggcgg catgagtacc cagtccggca attgcagcga gcgccagtaa aatgatgaaa
    11881 tatttttttg acttcaatga tgagcggcaa aaagctaaca cgtaaactcc acctatagac
    11941 aagcgcaacc agacaattac cgtgaaattg agcgacattt ctggcgataa ttagcagttg
    12001 gtgtaatatt aaaaatccta cgatgtcggc aaaatgcctc aaatttttgc caaatgcaaa
    12061 gcctaaataa gaaaaaatat aaaaatttca atatttacgt ctaatattag tttcttaagg
    12121 ttaagttaat attctatcct taaaatttcg ctccaaatgg caaaatatac acaacactct
    12181 ttatagcaaa tataagtgga caggtattca atggcggaag gaaaagcaat gtcagaattt
    12241 ttgccttttt cgcgaccagc aatgggcgtg gaggaactcg ctgcagttaa agaggttctc
    12301 gaatccggtt ggatcacaac cggtccgaag aatcaggcgc ttgagcaagc cttttgccag
    12361 ttgacgggaa atcagcatgc catcgcggtc agttcagcca ccgccggaat gcatatcacg
    12421 ctaatggcgt tggaaattgg caagggcgat gaagtgatta cgccttccct gacctgggtt
    12481 tcaaccctca atatgatttc cttgctgggt gcaacgccgg taatggtgga tgtcgaccgc
    12541 gatacgctga tggtcacgcc tgaagcgatc gaatcagcca ttacgccacg cactaaagcc
    12601 atcattccgg tgcattatgc cggtgcgcca gcagatattg acgccattcg cgccattggc
    12661 gaacgttacg gcatcgcagt tatcgaagat gctgcccatg ccgtcggtac gtattacaaa
    12721 gggcgacata ttggcgcaaa aggtaccgct attttttcat ttcatgccat taaaaatatt
    12781 acctgtgctg aaagtggcct gattgtaact gataatgaaa accttgcccg ccagctacgg
    12841 atgctgaaat ttcacggtct gggtgtcgat gcctatgaca gacaaacctg gggccgtgca
    12901 ccgcaggctg aagtcttaac accgggctat aagtacaatc tgaccgatat taacgccgcg
    12961 attgccctga cacagttagt caaattagag cacctcaaca cccgtcggcg cgaaattgcc
    13021 cagcaatatc agcaagcact ggcagctctc ccctttcagc cattaagcct tcccgcctgg
    13081 ccgcacgttc acgcctggca tctgtttatt attcgtgtcg atgaacaacg ttgtggtatc
    13141 agtcgcgatg cgttgatgga agcgttaaaa gaaagaggca ttggtaccgg gttacatttc
    13201 cgcaccgctc acacacaaaa atattatcgc gagcgttttc ccacgctgtc gttaccgaat
    13261 accgaatgga atagcgaacg catctgttcg ttgccgctgt tcccggatat gactaccgcc
    13321 gatgccgacc gcgtcatcac agcccttcag caactcgcag gacaataagc catgtttgaa
    13381 atccaccctg ttaagaaagt ctcggtggtt attcccgttt ataacgagca ggaaagctta
    13441 ccggaattaa tcaggcgcac caccacagcc tgtgaatcgt tggggaaaga gtatgagatc
    13501 ctgctgattg atgacggcag tagcgataat tccgcgcata tgctggtcga agcctcacaa
    13561 gcggagaaca gccatattgt gtctattttg attaaccgca attacgggca acattcagcg
    13621 attatggcgg gattcagtca cgttactggc gacttaatta ttacccttga tgccgatctc
    13681 cagaatccgc cagaagaaat cccccgcctg gtggcaaaag ccgatgaagg ttacgacgtg
    13741 gtagggactg tacgccagaa ccgccaggac agctggtttc gtaaaacagc ttcgaagatg
    13801 attaaccggc ttattcagcg caccactggc aaagcgatgg gtgactacgg ttgtatgctg
    13861 cgcgcctatc gccgtcatat tgtcgatgcg atgttgcact gccatgaacg cagcaccttt
    13921 atcccgattc tggcgaatat cttcgcccgc cgtgcaattg aaattccagt acatcatgcc
    13981 gagcgtgagt ttggtgaatc caaatacagt tttatgcgcc tgattaattt gatgtacgac
    14041 ctggtgacct gccttaccac aacgccgcta cgtatgctga gtctgctcgg cagcattatt
    14101 gcgattggag gttttagcat tgcggtgctg ctggtgattt tacgcctgac cttcggacca
    14161 caatgggcgg cagaaggcgt ctttatgcta tttgccgtgc tgtttacttt tattggcgct
    14221 cagtttatcg gcatgggatt actcggtgaa tatatcggca ggatctacac cgatgtccgc
    14281 gcccgccccc gctattttgt tcagcaagtt atccgtccat ccagcaagga aaatgaataa
    14341 tgaaaaccgt cgtttttgcc taccacgata tgggatgcct cggtattgaa gctctgctgg
    14401 ctgccggtta cgaaattagc gccattttta cccataccga taatcccggt gaaaaagcct
    14461 tttatggttc ggtggctcat ctagcggcgg aaagagacat tccggtttat gcgccggata
    14521 acgttaatca tccgctgtgg gtggaacgca ttgcccaact gtcaccagag gtgattttct
    14581 ctttttatta tcgccatctt atttgcgacg agatttttca gctcgctccc gcaggtgcat
    14641 ttaatctgca cggttcgctg ttaccaaaat atcgtggtcg cgcgccgctg aactgggtgc
    14701 tggtgaacgg tgaaacggaa acgggcgtta cattgcaccg aatggtaaaa cgtgccgatg
    14761 ctggggccat tgtggcgcaa ctgcgcgttg ccattgcgcc agacgatatc gctattacgc
    14821 tgcatcataa attgtgccat gccgcgcgcc agctactgga acagacatta cccgccatta
    14881 aacacggtaa tattctggaa atcgcccagc gcgaaaacga agccacctgt tttggtcgca
    14941 gaacgccgga tgacagtttc cttgaatggc ataaaccggc atccgtactg cacaacatgg
    15001 tacgtgccgt tgccgatccg tggccgggtg ccttcagcta tgttggcaat cagaaattca
    15061 ccgtctggtc gtcgcgtgtt catcctcatg ccagcaaagc acagccgggg agcgtgattt
    15121 ctgttgcgcc actgctgatt gcctgtggcg atggcgcgct ggaaatcgtc accggacagg
    15181 cgggcgacgg cattactatg cagggctcgc aattagcgca gatgctgggc ctggtgcaag
    15241 gttcacgctt gaatagccag cctgcctgca ccgcccgacg ccgtacccgg gtactcatcc
    15301 tcggggtgaa tggctttatt ggcaaccatc tgacagaacg cctgctgcgc gaagatcatt
    15361 atgaagttta cggtctggat attggcagcg atgcgataag ccgttttctg aatcatccac
    15421 attttcactt tgtcgaaggc gatatcagta ttcattccga atggattgag tatcacgtca
    15481 aaaaatgtga tgtcgtcttg ccgctggtgg cgatagccac gccgattgaa tatacccgca
    15541 acccgctgcg cgtatttgaa ctcgattttg aagagaatct gcgcattatc cgctactgcg
    15601 ttaagtaccg taagcgaatc atcttcccgt caacttcaga agtttatggg atgtgtagcg
    15661 ataaatactt cgatgaggac cattctaatt taatcgtcgg cccggtgaat aaaccacgct
    15721 ggatttattc ggtgtcaaaa caattacttg atcgggtgat ctgggcctat ggcgaaaaag
    15781 agggtttaca gttcaccctc ttcctcccgt ttaactggat gggaccacga ctggataacc
    15841 ttaatgcagc gcgaattggc agctcccgcg ctattacgca actcattctc aatctggtag
    15901 aaggttcacc aattaagcta attgatggcg gaaaacaaaa acgctgcttt actgatatcc
    15961 gggacggtat cgaggcgttg taccgcatta tcgaaaatgc gggaaatcgc tgcgacggcg
    16021 aaattatcaa cattggcaat cctgagaacg aagcgagcat tgaggaactg ggcgagatgc
    16081 tgctggcgag cttcgaaaaa catccgctgc gccatcattt cccaccgttt gcgggctttc
    16141 gcgttgtcga aagtagctgc tactacggca aaggatatca ggacgtagag catcgtaaac
    16201 cgagcatccg caatgcccac cgctgcctgg actgggagcc gaaaattgat atgcaggaaa
    16261 ccatcgacga aacgctggat ttcttcctgc gcaccgttga tcttacggat aaaccatcat
    16321 gaccaaagta ggcttacgca ttgatgtcga tacctttcgt ggcacccgtg aaggcgtgcc
    16381 gcgtctgctg gaaatcttga gtaagcataa tattcaggcc agcatttttt tcagcgtcgg
    16441 cccggacaat atgggccgcc atctctggcg actggtgaag ccacagtttt tgtggaagat
    16501 gctgcgctca aacgcggcat cgctttatgg ctgggatatt ttactggcag gtacggcctg
    16561 gccaggtaaa gagattggtc atgccaatgc cgatatcatt cgtgaagcgg ctaaacatca
    16621 cgaagtcggt ctgcacgcct gggatcacca tgcctggcaa gcccgtagcg gtaactggga
    16681 tcggcaaaca atgatcgacg atattgcacg cggtcttcgc actctggaag agattatcgg
    16741 tcaaccggta acctgttctg ccgctgcggg ctggcgtgcc gaccagaagg tgatcgaagc
    16801 aaaagaagcg ttccatttgc gctacaacag cgattgtcgt ggggccatac cgttccgtcc
    16861 actgcttgaa tcaggaaacc ctggcactgc gcaaattccg gtgaccttac ccacctggga
    16921 tgaagtgatt ggtcgggatg tgaaagcaga agattttaac ggttggttac tcaaccgcat
    16981 cctgcgagat aaaggcacgc cggtttatac cattcatgca gaagttgaag gctgcgctta
    17041 tcagcataat tttgtggatc tcctcaaacg cgcagctcag gaaggcgtga cattttgccc
    17101 tttaagcgaa ctgttatcag agacgttgcc gctcggacaa gttgttcgcg gaaatattgc
    17161 cggacgtgaa ggctggctgg gttgccaaca aattgcgggt agtcgctgat gaaatcggta
    17221 cgttacctta tcggcatctt cgcgtttatt gcctgctatt acctgttacc gatcagcacg
    17281 cgtctgctct ggcaacccga tgaaacgcgt tatgcggaaa tcagtcgaga aatgctggca
    17341 tccggcgact ggattgtgcc ccatctgtta gggctacgtt atttcgaaaa acccattgcc
    17401 ggatactgga ttaacagcat tgggcaatgg ctatttggcg cgaataactt tggtgtgcgg
    17461 gcaggcgtta tctttgcgac cctgttaact gccgcgctgg tgacctggtt tactctgcgc
    17521 ttatggcgcg ataaacgtct ggctctactc gccacagtaa tttatctctc attgtttatt
    17581 gtctatgcca tcggcactta tgccgtgctc gatccgttta ttgcattctg gctggtggcg
    17641 ggaatgtgca gcttctggct ggcaatgcag gcacagacgt ggaaaggcaa aagcgcagga
    17701 tttttactgc tgggaatcac ctgcggcatg ggggtgatga ccaaaggttt tctcgccctt
    17761 gccgtgccgg tattaagcgt gctgccatgg gtagcgacgc aaaaacgctg gaaagatctc
    17821 tttatttacg gctggctggc ggttatcagt tgcgtactga cggttctccc ctggggactg
    17881 gcgatagcgc agcgggagcc tgacttctgg cactattttt tctgggttga gcatattcaa
    17941 cgctttgcac tagatgatgc ccaacataga gctccgttct ggtactacgt gccggtcatc
    18001 attgccggta gcctgccgtg gctgggatta ctccccggtg cactgtacac aggctggaaa
    18061 aaccgcaagc attccgcaac cgtctatttg ttgagctgga cgataatgcc gctgctgttt
    18121 ttctccgtcg ctaaaggcaa attgcccacc tatattcttt cttgctttgc acctctggca
    18181 atgctgttgg cgcattacgc gttgctggca gcaaaaaata atcctctggc gctgcggatt
    18241 aatggctgga ttaacatcgc ttttggcgtc actggcatta ttgccacatt tgtgatctcc
    18301 ccctggggac caatgaacac gccggtgtgg caaaccttcg agagctataa agtcttttgt
    18361 gcctggtcga ttttttcgct atgggcattt ttcggctggt acaccttaac aaacgtcgaa
    18421 aagacctggt ctttcgccgc gctttgcccg ctggggctgg cgttgctggt aggattttca
    18481 attcctgaca gagtcatgga aggaaaacat ccgcaatttt ttgtcgagat gacacaagaa
    18541 tcacttcagc caagccgcta tattcttact gatagcgtcg gtgttgccgc aggtctggca
    18601 tggagcctgc aacgcgatga catcatcatg tatcgccaga caggtgagtt gaaatacggc
    18661 cttaattatc cggatgcgaa agggagattt gtcagcggtg atgagttcgc aaactggctt
    18721 aatcaacatc gtcaggaggg gattattaca ctcgtgcttt cggttgaccg cgatgaagat
    18781 atcaacagtc tcgccattcc gcccgcagat gccatcgttc gtcaggagcg tctggtgctg
    18841 attcagtatc gtcccaaatg atctggctaa cattagtctt tgccagcttg cttagcgttg
    18901 ccgggcagtt gtgtcagaaa caggcaacct gctttgtggc gataaacaaa cggcgcaaac
    18961 atatcgtgct gtggctggga ctggcgctgg cttgtcttgg tcttgccatg atgctctggc
    19021 tgctggtctc gcagaacgta ccggtaggca ttgcctaccc gatgttaagt ctgaattttg
    19081 tctgggtgac gctggctgca gtaaaactgt ggcacgaacc ggtatcgccg cgtcactggt
    19141 gtggggtggc gttcattatt ggtggcattg tgatcctcgg gagtacggtg taatgggcct
    19201 gatatgggga ttattcagcg tcattattgc ctcagttgcg caattaagcc tgggttttgc
    19261 ggcaagtcat ctgccgccga tgacgcactt atgggatttt atcgcggctc tgttagcttt
    19321 tggcctggat gcccggatac tgttactcgg gctgctgggt tatctgcttt ccgttttttg
    19381 ttggtataaa acgctacata aacttgcgct aagcaaagcc tatgccttgc tcagtatgag
    19441 ttatgtgctg gtatggattg cttcaatggt acttcccggc tgggaaggga ctttttcgct
    19501 gaaagcacta ctgggagtag cctgtattat gagcgggttg atgctgattt tcctgcccac
    19561 gacaaaacaa cgttactgag ttttccctgc cgctgtacac tgccgttccc actcatcggg
    19621 gctataactg cttgccgaga ggactttcac cgtatgcagc ttttcacgat taatacaata
    19681 aagcgcattc tgcaaaggcg agagtaaatc ccccactttc acggcgaagt cactcttcac
    19741 ttcggcgatc atttttatcg cgccgccagc atcgcagagc ataagtacat gtctgttatc
    19801 ttgtttattg cagcacgatt ttttgtgcca gccattccat tgcattatcc tgtttgctaa
    19861 gagttttcac atcaatttac agattaacag ataaactctt tatcaacggc aatggaatgg
    19921 cagaaatgag aagtgaaacg gagggttaat tattgctgac gttgcaccca ctcttttagc
    19981 gcctgacgtg aaattttaat accgccgttt ttcagctccg gcggtaaagt cagccagcgc
    20041 accggttgtt gaaaacgagc cagcttatct ttcacccatt cgccaagatc aacggtttgc
    20101 tggtcatact ccaccaccgc taccggccga tgaccaaact ccttgtcggc aacggaaacg
    20161 ataaacacct gcaaaaccgc aggatgtgca gcaataacac gctcgacttc ttccggctga
    20221 ataccctcac cgccactgaa gaacagattg tctaaacgtc cgacaatggt cagcttgcca
    20281 ttatgcatct caccgcgatc gcgcgtagcg taccagcctt cgtcattaac cagtgaaacc
    20341 agttgcccgt tacgccagta accttctgcc atactggcag cccgcagcca cacttcatta
    20401 ttaacgattt tcacttcccg acccggcagc ggcaaaccaa cgtctgccag gccgtcggct
    20461 tctttcgcac ataccgtgga ggcaaactcg gtcagaccat agccgcaaaa gcaacgaatc
    20521 ccctgctcgc gcgcctgttc cgtcaactcg accgggatag ccgcgccgcc aagtaacacc
    20581 gctttcaggg aaacggaact gcggttaacc agcaaacgcc agagttgtgt tggcaccagt
    20641 gaagcgtgag tacagcctgc cagcatttgc tccaatggct gtttatcacg caccgtcatc
    20701 cgcgcaccag cgtataacca gcgccacata attccctgac cggagacgtg aaacagcggt
    20761 aaagagagca accagtcatc gtgatcgcca aacggaatca gcgataacac accttgcgca
    20821 ctggcaagat gggcctgata agtatggaca gcggctttcg gcaaaccggt agaacccgag
    20881 gtcaacgtca ttgagcacag acgcgtcggc tgccacgcag cggcatgtac gccttcaacc
    20941 agctgaatgt gcagcgacgt taatgccgga aacgtgtttt ccccatccgg caccagcgca
    21001 aattgcagcg tcagattggg cagcaattct tcaagcaacg gttgcggcag ctgagggttc
    21061 acgggcaaca cccgcgcccc gcattgcagt aacgccagcc aggcgagcag cgtttgcggc
    21121 gtattccacg cccgcaacat cacgccgctg ccctcaacca ccccctgcgc cgcaaagccc
    21181 gtagctaact catcgacgcg agcacaaagc tcgcgccagt tgagttgctc gtcattaaga
    21241 cgtaaggcga tggcttctcc ccgcacttgc cgccagtgac gccacggcca gtcagagaag
    21301 atcataacaa ctgctcctgt acatcagcgt ccactaacgg cagtgggctc cccggccagc
    21361 gacgaacctg ctgcgcctgc atcagatcca gcgtgtccag tcccggaatg gtgtctggcg
    21421 ttaaccaggc agcaatccgc gccagttgcg ttagacctaa gctcgactca atggaagaac
    21481 tgatcaccgc cgtcagcccc agcgcatgcg ccgcctgtac ctgctcgcgt actttttcca
    21541 gactgcccgt gagcgtgggt ttgataacta ccgcgcgcac gccctcttca gccacaaagg
    21601 caaaatccgg ctcgcgcagg ctttcatccc aggcaatggc aatgccggtt tcacgggcaa
    21661 acgctcgcga atcatcgcgg gttttgcacg gctcttcgag aaacgcgatg cggtggcgat
    21721 aatccgggtt aacatatttg gcaaactgct gacctttcag cggtgtccag gcgcgatttg
    21781 cgtcaagacg caaatgcaga tccggaattg cctccagcaa cagattcacc accatgccgt
    21841 cgcgcaccgc ttcgtacaat cccactttga ccttcgccac tttctcgccc ggcatatcgg
    21901 caagtttgag gatcagatcg tccggatcgc cattacacag cggtgccgca cggtagttgg
    21961 ctgcttgcgg caacgtatct gtcagttctg ccaatgcaca gcttacgcca aaggccacgg
    22021 aaggcatctg cggtagctcg caatcgcctg ccagccagtt atttacccag gcaagcagca
    22081 cgctttgcgc ctcttcccag ttttcctggc tgaagcccgg cagtggggag atctcccccc
    22141 acccttcgcg ctcgccctca cgcaggcaaa cgtacagccc gtcgcgggtt tttaaccgcc
    22201 tgtcgcgcag aaccaccccc gcgtccatgg ggatctgcca gcggtatacc tgcgcgctac
    22261 gcattacgga ttccgtttaa atttgctgaa atcaggctga cgtttctggt tgaaagcgtt
    22321 gcgaccttcc tgaccttctt ccgtcatgta gaacagcatg gtggcgttgc ccgccagctc
    22381 ctgcagcccc gcctgcccgt cacagtcggc gttcagcgca gctttcaggc agcgcagcgc
    22441 catcgggctg ttttgcagca tttcgcggca ccaacggacg gtttcttttt ccagatccgc
    22501 cagcggtacc acggtgttga caaggcccat atccagcgcc tgttttgcgt cgtactgacg
    22561 gcacaggaac cagatttctc gcgctttttt ctgcccgacg atacgagcca tgtaggaagc
    22621 gccccagccg ccgtcgaagg aaccgacttt cgggccagtc tgaccgaaga tggcattatc
    22681 tgctgcgata gtcaggtcgc acatcatgtg cagaacgtga ccaccgccga tggagtagcc
    22741 agccaccatc gcgacaaccg gtttcggaca ggtacggatc tgacgctgga agtccagcac
    22801 gttcaggtga tgtacgccgg aatcatcttt atagccgccg taatcaccac gcactttctg
    22861 gtcaccaccg gagcagaacg ctttatcgcc tgcgccagtc agaatgatca cgccgatatt
    22921 gtcgtcataa cgcgcatctg ccagtgcctg gatcatctct tttaccgtca gaggacggaa
    22981 ggcattgcgc acctgcggac gattaatggt gatttttgcg ataccgtcgg tggatttttc
    23041 ataacgaatg tcctcgaaac cttcggagca gtcgtgccat tcaaccggtg cgtaaggcat
    23101 tgcttcatca ggataaatca tattgtgtcc tttagtcaga aacgcaagat ctgcgccaga
    23161 cttgcgatta cgccagcggg attttcccga tgcgcgttat gtccggcgcg aggaatgaca
    23221 tggcagtcgg cagccagttc cgccgccagg gcgcggaatt tgctgtcacg ttcaccacat
    23281 aaataataaa acgcaaatgt gcgggcgcta aggttagcac gtaaatcagg ctggacggcg
    23341 agagaagtcg cctccagcat ggcggcaagg gttgcgccat tattgttgct gcgcagcgcc
    23401 accagctccc ggcgttgatc gtcattgagc gaggcaaaaa caggctgttg ataccagtcg
    23461 gcaaataccg ccgttaacgg ttctgtgcga aaacgctgcg cccattggcg atcggaacgc
    23521 tgacgttccg cacgttgttc agcattttgc agccccggat gcccgccttc gacaataacc
    23581 ccacaaagcc ccgccagccc ctggcaagcc gccatcatcg ccacccgtcc gccaagcgag
    23641 taccccacca gccagaagtc aaggatgttg taactaacca aggttttacg cagtaagtcg
    23701 gtgacatcat caaatccatc gacgctaatc gccgccgaac caccatgacc taggagatca
    23761 acatacaacc gtgagtagtc ggcaaacgcc tcgcccactt cttgccattc gtggcaatcg
    23821 ccggaaaaac cgtgcaaaaa caccagccag ggtaaaccta gttttccgtg ttttgcctgc
    23881 gcgtgcagga tcataaatgg cttacctgcg ccagaagttg ctggagcgtt tgcgcaccat
    23941 cggtgtcgtt aaccaccatt tcaatcaccg tggtggttgg cgtgcgccag gcgtcggcaa
    24001 atgccgtttc aagttcctgc cagttttgcg gatgatgata tttcagctcg aacatcgcgg
    24061 cggcgtgctc aaaatggacg ttttgcggca tcagatagaa acgctcacgc tcgctttgcg
    24121 gcgttggcaa cagcgagaaa atttgcccgc cgttgttgtt caccacaatt aataccagcg
    24181 gcgcagaaac ctgacgcaat aacgccagcg cgttgagatc gtaaagcgcg gagagatcgc
    24241 ccacaatcgc cagcgtcggt ttgccgcttg cccgctgaac gcctgcggcg gtcgaaagca
    24301 gtccgtcgat accgctggcc cctcggttgc tgtacaccgg gtaacctgcc ggaagttgcg
    24361 aaagcgcatc aatcagacgt accaccaggc tgttaccaac aaacaattgc ccctgttcag
    24421 gcagatagtc gctgatgcga tgcgccagtt gcgcttcgcc aaacgcatca cggcgggcaa
    24481 taaccgcctg cattgcctgt tccgccaggc gcgggatttc aacgcaccag ggctggcgtt
    24541 tttctgccgg atgcagctcc agccagtcgg caatattggc aattaagcga cgtccgcgat
    24601 ggtgtgccgg atcaagtcgc ccttcaatgt catcaacaat ccagtactct tctggttcac
    24661 agcttgcctg ccattgcagg agccgtttcc ccgtcaggct acttcccagt tgcaccacaa
    24721 tttgcgcctg ctgcagctcg ctggtcgctt tggcattgcc taaccaaaga tcggcacacg
    24781 gcagcggctg cccggtttgt gacagcacat cgccaatcag cggccagcca agagtttgcg
    24841 cccacagggc aacttttttg ccctcttccg cactcatgcg cccggcaacc accacgccgc
    24901 gcttttgtcg ccagaagaac cagtcgcgct gtttttcact ttccagacga ggcgcttcac
    24961 gcagccacgg tttgtcgtcc tgccaccagt cacccagacg ctgttgccag ctaagcccgg
    25021 tatcgtccat ttcgccatac agcggttcag caaacgggca gttgatatgg actccccccg
    25081 catgaagcgt accgagagcg tggtcgatgg tagaaaccag ccaacgtgcg gggatatcct
    25141 gggtcgggcg cggcaatgaa atactgtgcg tggggtgaga ggcgaacatt cccggctggc
    25201 gaattgcctg attcgcgccg cagtcaatta gctccggcgg gcgatcggcg gttaagagaa
    25261 tcagtttttc tccggttaac ccggcttcaa tcagtgccgg atagagattt gccaccgccg
    25321 tgccggaggt cacaatcacc gccaccggct gcttgctgac tttcgccagc cccagcgcca
    25381 gatgccccaa cccacgctca tcgaaatggg tgtggtgaat gaatgcggaa ttctccgccg
    25441 ccgctaacgt taacggtgta gaacgcgagc ctggggcgat acagatgtgt ctgacgccgt
    25501 gacgcgttaa tgcttccaga atgaccgccg cccagcgtcg gttaaatgcg cttactgaca
    25561 tgagtttgtc cggtatcaat attgcggcta agtataagga gctacaaaaa tagattattg
    25621 atatgaatcg gtaatgatgc gactcattac tattccattt gtaataaagt acgcagccct
    25681 gccgctttgt tgtcgatttc ctgccactct tgctcggggt cggaaccacg gacaatgccc
    25741 gcgccagcat ataatcgcac gacattgccg ctaatttttg ctgagcgcag ggaaacgcag
    25801 aattcgcttt gttgtaatga gagatagccc gcagaaccgg cgtaccattc tcgggtgaac
    25861 ggttcgtgac gggcgataaa ctgtcgcgcc agatcgcgcg gtaagccagc aactgctgcc
    25921 gttggctgca actgatgtaa acagatcaca tcatccgctt tgttgagtga agtccagata
    25981 cagcggcgaa gatgctgcac tttacgcaga cgcagtacct gcggcggtaa aacatccagc
    26041 gtctgggtat cggcctgtaa tcgttggcag atatcttcca ccaccagcat gttctcgcgc
    26101 tggtttttat catccgccat cagccactct cctaactgct gcgcctgctt atcatcagga
    26161 tgatttgcta ctgttcccgc cagcgcttca gtacgcagcg ctttgtcacg ccgccgccat
    26221 aaccgttccg gtgaagagcc aagaaaagca ttttcgccat caaaggccat gtaaaaatgg
    26281 tagcaattca gattcagtcg acgactggca gccatcatcg ccgccgcgtt gaccggactt
    26341 gcgaaatgca ggtcagttgc ccgagcgagc accactttgt cgagctcacc ttcggcgatg
    26401 gttttcgttg ccagttcgat taattgcgtc cagcccgttt tgtccggcca gtgttgttct
    26461 cgcgtggtgg ttaaatgtaa cccaggcaag ggcttgatac tcaccagtgt ggcgataaat
    26521 tcttttgcct gaatcgcatc gtgctgaagg gagctttcgc tgaatagcgt cagccgcagc
    26581 gtggctttac cgccacagcg tcgccattcc aggcggggta aaagtaaatt gccctgcgac
    26641 gggtcaaaag cattcagccc ccaaatgcgt aagtcagcgt gttccgggtg ctggcgaaga
    26701 aaacgttgtg cctggtccaa cgacgtaaaa cgggtaatcg cgcccaggac ggcagcttct
    26761 tcatcaccat tacgttgttg ccagtagaat tgcgggtacg tttgctgact ggccagccag
    26821 ctcaaggcat caaaagcgtc tttgagaggg aaaggaatat cgataacccg aatgccgggt
    26881 gtcgccggaa tctcttgcga caaatggcgc agtagatttt ccagcgccgt agtaagtgat
    26941 tgcacgcgga cctctccctg ttaaaagcct cacattatac ggggtactac aaaaaaaatg
    27001 cagtaccccg gtgtagggag gttttaacgg cgtgccagca acagtcctag taccagcccg
    27061 acggccgcgc ccacaccaat tccttgccag ggtttttcgt ggacgtagtc atcagcacga
    27121 taaacagcct gcttcgcccg atagtaataa ctgtctgaag cctggctaac ccgttttttc
    27181 acatcatcca gcgctttttc tgcatgcgct ttcagctcaa catatttctg atcggcggga
    27241 tcgccagagg agcggagcac ctcttccagt gtttcactaa gcagcgtcag gtcgtcatcg
    27301 atacgtgtat caccaaactg attagacatt ctcgttctcc atttgcgtaa aacctgtcac
    27361 taactataga caagagttgt tgattacgcc tgaattacct cgcgtgccat cccaatgtgt
    27421 gggataccat cttcttcata gacctccgtc acagggataa aaccgaaact ctggtagaag
    27481 ttttgcagat gcgcctgcgc cccgaggtaa acaggcttat caggccagtg atgcgtacat
    27541 gtttcgagtg ttttactcat cagctgctgc cccacttttt caccacgcaa cgcttcactg
    27601 acaatcaccc gacctataac gaccggctca agatcatcat cacttttcag aatcctcgca
    27661 tacgccacca gttcatcatt tttccaaccg agaatatggc gattatcccc cgtcaaatca
    27721 tcaccatcga tatcctgata agggcaattc tgctcgacaa caaacaccgc gcaacgcagt
    27781 tgtaataagg cataaagctg ggaaaccgat aattcagagt gatgcagatc ttgccattca
    27841 atcataatgc tctcctttta gatgtatgcc ttatactagc cggaatattt tttgaaacgt
    27901 gatgaactca tggaattaat ttttttaggt acttcagccg gtgtgccaac ccgcacgcgc
    27961 aatgtcacgg caatattgct taacctgcaa catccgaccc agagcggact ttggttgttt
    28021 gactgcggtg aaggcaccca gcatcagcta ctgcataccg cctttaaccc tggaaaactg
    28081 gacaagattt ttatcagtca ccttcatggc gatcatcttt ttggtttacc cggcttgctg
    28141 tgcagtcgtt ctatgtcagg cattatccaa cccttaacga tttatggtcc gcaaggtatc
    28201 cgtgaatttg tggaaaccgc gctgcggatt agcggctcat ggaccgatta tccgctggaa
    28261 attgtcgaaa ttggcgctgg cgaaattctc gatgatggcc tgcgcaaagt aaccgcttat
    28321 ccgctggaac acccactgga atgttatggc tatcgtattg aagaacatga taaaccgggt
    28381 gcattaaatg cccaggcatt aaaagctgct ggcgtgccgc ctggcccact gtttcaggaa
    28441 ttaaaagcgg gcaaaacaat cacgctggaa gatggaaggc agattaacgg cgcagattac
    28501 ttagctgctc cagtgccagg taaagcgctc gctattttcg gcgataccgg cccctgcgat
    28561 gccgcacttg acctggctaa aggtgtcgat gtcatggtgc acgaagcgac gctggatata
    28621 accatggaag ccaaagccaa tagtcgcggc catagctcta cacgccaggc tgcgacacta
    28681 gcccgtgagg ctggagtcgg caagctaatc attacccacg tcagctcgcg ctatgatgac
    28741 aaaggttgtc aacacctgtt acgtgaatgc aggtcaattt tcccggcgac tgaactggcg
    28801 aatgatttca ccgtgtttaa cgtttaactc tgcttattat taaaaacagg gtgaaacttg
    28861 ccctgttata gcaacccgcg ctacatatat ccacccacac aggcatattg agcgattttt
    28921 gatatttatt catctcctta cgcactatat tcagagcaat aagttgcctc ttttcatctt
    28981 gattcagcac aacctttgca actaaaagga gcccatgatg gttacagttg tcagcaatta
    29041 ttgtcaatta tctcaaaccc aactcagtca gacatttgca gaaaaattta ctgtgaccga
    29101 ggaattactg cagtctttaa aaaaaacagc gttatccgga gatgaagaaa gtattgagtt
    29161 actgcataat attgcgttag gttatgatga atttggaaaa aaagctgggt aatgctgcca
    29221 acttactgat ttagtg
//
LOCUS       DAJTQY010000048        29049 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.048, whole genome shotgun sequence.
ACCESSION   DAJTQY010000048 DAJTQY010000000
VERSION     DAJTQY010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29049
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.048"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            109..1140
                     /gene="mdtN"
                     /locus_tag="OO349_002170"
     CDS             109..1140
                     /gene="mdtN"
                     /locus_tag="OO349_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HCR5867229.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWLVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMTTVRAPFDGR
                     IISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            1140..3188
                     /gene="mdtO"
                     /locus_tag="OO349_002171"
     CDS             1140..3188
                     /gene="mdtO"
                     /locus_tag="OO349_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HCR5867230.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYG
                     EPLIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIYGQTFPAMLDYPEVVVRLTLWCIV
                     VGLYPTLLMTLIGVLWFPNRAITQMHQALNDRLDDAISHLTDSLAPLPETRIEREALA
                     LQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKLA
                     SEINKLQHAVAEGQCWQSDWRISESEAVAARECNLENICQTLLQLGQMNPNTPPTPAA
                     KPPSMVADAFTNPDYIRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVGS
                     SYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLAAWIATSSERSS
                     YIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAMIYTFVWPESEARTL
                     PQKLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLDS
                     EERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGLA
                     TALSRSPQITLEETPTSQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGATQ
                     "
     gene            3185..4651
                     /gene="mdtP"
                     /locus_tag="OO349_002172"
     CDS             3185..4651
                     /gene="mdtP"
                     /locus_tag="OO349_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HCR5867231.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAEHVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            4849..5088
                     /locus_tag="OO349_002173"
     CDS             4849..5088
                     /locus_tag="OO349_002173"
                     /inference="COORDINATES: protein motif:HMM:NF016750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5867232.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVS"
     gene            5098..5268
                     /locus_tag="OO349_002174"
     CDS             5098..5268
                     /locus_tag="OO349_002174"
                     /inference="COORDINATES: protein motif:HMM:NF012602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HCR5867233.1"
                     /translation="MNYVAERLSAIKEKYGPDAIQTTGSSRGTGNETNYVMQKFARAV
                     IGTNNVDCCARV"
     gene            5317..6729
                     /gene="fdhF"
                     /locus_tag="OO349_002175"
     CDS             5317..6729
                     /gene="fdhF"
                     /locus_tag="OO349_002175"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HCR5867234.1"
                     /translation="MSNAINEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIV
                     CDPRKIETARIADMHIALKNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKI
                     VEGYTPESVEDITGVSASEIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLA
                     MLTSNLGKPHAGVNPVRGQNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVES
                     LPAHTGYRISELPHRAAHGEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFI
                     TKTASAADVILPSTSWGEHEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMG
                     YPMHYNNTQEIWDELRHLCPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKF
                     DTPNGLAQFFTCDWVAPIDKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPG
                     YAQINTEDAKRLGIEDEALV"
     gene            6730..6996
                     /locus_tag="OO349_002176"
     CDS             6730..6996
                     /locus_tag="OO349_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase"
                     /protein_id="HCR5867235.1"
                     /translation="MHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTESLSP
                     ITKTPEYKYCAVRVEPISDQRAAEQYVIDEYNKLKTRLREAALA"
     gene            7090..7779
                     /locus_tag="OO349_002177"
     CDS             7090..7779
                     /locus_tag="OO349_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCR5867236.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(7857..9170)
                     /gene="gltP"
                     /locus_tag="OO349_002178"
     CDS             complement(7857..9170)
                     /gene="gltP"
                     /locus_tag="OO349_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HCR5867237.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVAGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(9512..10108)
                     /gene="nrfG"
                     /locus_tag="OO349_002179"
     CDS             complement(9512..10108)
                     /gene="nrfG"
                     /locus_tag="OO349_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HCR5867238.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            complement(10105..10488)
                     /gene="nrfF"
                     /locus_tag="OO349_002180"
     CDS             complement(10105..10488)
                     /gene="nrfF"
                     /locus_tag="OO349_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HCR5867239.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVSEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            complement(10481..12199)
                     /locus_tag="OO349_002181"
     CDS             complement(10481..12199)
                     /locus_tag="OO349_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HCR5867240.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQSDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLCLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQARGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTRADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            complement(12219..13175)
                     /gene="nrfD"
                     /locus_tag="OO349_002182"
     CDS             complement(12219..13175)
                     /gene="nrfD"
                     /locus_tag="OO349_002182"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HCR5867241.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPSLPVLLLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            complement(13172..13843)
                     /gene="nrfC"
                     /locus_tag="OO349_002183"
     CDS             complement(13172..13843)
                     /gene="nrfC"
                     /locus_tag="OO349_002183"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HCR5867242.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHVPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            complement(13840..14406)
                     /gene="nrfB"
                     /locus_tag="OO349_002184"
     CDS             complement(13840..14406)
                     /gene="nrfB"
                     /locus_tag="OO349_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HCR5867243.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAVQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQASPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            complement(14451..15887)
                     /gene="nrfA"
                     /locus_tag="OO349_002185"
     CDS             complement(14451..15887)
                     /gene="nrfA"
                     /locus_tag="OO349_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HCR5867244.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHHDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            16280..18238
                     /gene="acs"
                     /locus_tag="OO349_002186"
     CDS             16280..18238
                     /gene="acs"
                     /locus_tag="OO349_002186"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HCR5867245.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAMHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGNIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            18438..18752
                     /locus_tag="OO349_002187"
     CDS             18438..18752
                     /locus_tag="OO349_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HCR5867246.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            18749..20398
                     /gene="actP"
                     /locus_tag="OO349_002188"
     CDS             18749..20398
                     /gene="actP"
                     /locus_tag="OO349_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HCR5867247.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTSADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVADLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMLGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            20576..21868
                     /locus_tag="OO349_002189"
     CDS             20576..21868
                     /locus_tag="OO349_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCR5867248.1"
                     /translation="MRYNGLNNMFFPLCQINDNHSITSSSHTKKTKSDNYSKHHKNTL
                     IDNKALSLFKIDDHEKVIGLIQKMKRIYDSLPSGKITKETDRKIHKYFIDIALYANNK
                     CDDRITRRVYLSKEKDVSIKVVYFINNVAVHNNTIEIPQIVNGGYDFSHLSLKGIVIK
                     DEDLSNSNFAGCRLQNAIFQDCNMYKTNFYYAIMEKILFDNCILDDSNFAQIKMADGT
                     LNACSAMHVQFYNAAMNRANIKNTFLDYSNFYMAYMAEVNLYKVIAPYVNLFKADLSF
                     SKLDLINFEHADLSRVNLNKAILQNINLIDSKLFCTWLTNTFLEMVICTGSNMANVNF
                     NNANLSNCHFNCSILTKACMFNTRLYRVNFDEASVQGMGISILRGEENIPIDSDTLVT
                     LQKFFEEDCTSHTGMSQTEDNINAVAMKITADIMQHAD"
     gene            complement(22022..23671)
                     /locus_tag="OO349_002190"
     CDS             complement(22022..23671)
                     /locus_tag="OO349_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HCR5867249.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(23822..25171)
                     /gene="ghxP"
                     /locus_tag="OO349_002191"
                     /gene_synonym="yjcD"
     CDS             complement(23822..25171)
                     /gene="ghxP"
                     /locus_tag="OO349_002191"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HCR5867250.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(25877..26341)
                     /gene="soxR"
                     /locus_tag="OO349_002192"
     CDS             complement(25877..26341)
                     /gene="soxR"
                     /locus_tag="OO349_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HCR5867251.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            26427..26750
                     /gene="soxS"
                     /locus_tag="OO349_002193"
     CDS             26427..26750
                     /gene="soxS"
                     /locus_tag="OO349_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HCR5867252.1"
                     /translation="MSHQKIIQDLIAWIDVHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(26753..28330)
                     /gene="pdeC"
                     /locus_tag="OO349_002194"
                     /pseudo
     CDS             complement(26753..28330)
                     /gene="pdeC"
                     /locus_tag="OO349_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
     gene            28759..29040
                     /locus_tag="OO349_002195"
     CDS             28759..29040
                     /locus_tag="OO349_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HCR5867253.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
BASE COUNT         7288 a         7779 c         7348 g         6634 t
ORIGIN      
        1 cgggcaaata tcaatctggc atttgtcgga attatttata ccgccctttt tgctctctac
       61 gccatgctgt tctggctggc atttttctaa tctatttgag acgcgctcat ggaaagtacg
      121 ccgaaaaaag cacctcgcag taaattccct gctctgttag tggttgcgtt ggcgctggtt
      181 gcccttgttt tcgttatctg gctcgtagac agtgcgccat caactaatga cgcttacgcg
      241 tcagcagata ccattgatgt ggtgccggaa gtcagcggtc gcattgtaga actggcggtc
      301 accgacaacc aggcggtgaa acagggcgat ttgctgttcc gcatcgaccc gcgcccgtac
      361 gaagccaacc tggcgaaagc cgaagcctcc ctcgcggcgc tggataagca aattatgctc
      421 acccagcgta gcgttgacgc ccaacagttt ggtgccgact cggttaatgc cacggtagaa
      481 aaagcccgtg ccgccgcgaa acaggccaca gatacattac gccgcaccga gccattactg
      541 aaagagggtt ttgtctcagc agaagacgtt gaccgtgcaa gaacggcgca gcgcgccgca
      601 gaagcggatc ttaatgccgt attgttacag gcgcagtcag ccgccagcgc cgtcagcggc
      661 gtcgatgcgc tggtggcgca acgtgcggcg gtcgaagcgg atattgccct gaccaaacta
      721 catctggaaa tgacgaccgt tcgcgcgcca tttgatggcc ggatcatttc cctcaaaacc
      781 tccgtcgggc aatttgcttc tgccatgcgc cctattttta ccctcatcga cactcgtcac
      841 tggtacgtga tcgccaactt ccgcgaaacc gatctgaaaa atattcgctc aggtacaccc
      901 gcaacgattc gcctgatgag tgacagcggc aaaaccttcg agggtaaagt ggattcgatt
      961 ggctacggcg tgctaccgga tgacggcggc ctggtgctgg gcggcctgcc aaaagtgtct
     1021 cgttctatta actgggtccg cgttgcccag cgttttccgg tcaaaatcat ggtcgataaa
     1081 cctgacccgg aaatgttccg catcggcgct tcggcagtcg ctaatcttga gccgcaataa
     1141 tgagcgcgct caactccctg ccattaccgg tggtcaggct gctggcgttc tttcatgaag
     1201 agttaagcga gcggcgaccc ggccgcgtgc cgcagatcgt gcaactctgg gtaggctgcc
     1261 tgctggtgat tctgatctcg atgacttttg agatcccttt tgtggcgtta tcgctggcag
     1321 tgctgtttta cggtatcggt attcagtcga acgcgtttta caccaaattt gtggcgatct
     1381 tgtttgtggt tgccacggtg ctggagatcg gcagcctgtt tttgatctac aaatggtcat
     1441 acggcgaacc gttgatccga ttgatcatcg ccgggccgat cctgatgggc tgcatgtttt
     1501 tgatgcgcac ccatcggctg ggactggtct ttttcgctgt cgccatttac gggcaaacct
     1561 tccccgccat gctcgactat ccggaagtag tcgtgcgctt aacgctgtgg tgtatcgttg
     1621 ttggcctcta tccaaccttg ctgatgacgt taatcggcgt gctgtggttt cccaatcgtg
     1681 ccattacgca aatgcatcaa gcgcttaatg atcggcttga tgatgccatt agccacctga
     1741 cggacagcct cgcaccgcta cccgaaacgc ggattgaaag agaggcgctg gcgctgcaaa
     1801 aactgaatgt cttttgtctc gcggacgatg ccaactggcg aactcaaagc gcatggtggc
     1861 aaagctgcgt ggcaacggta acctacattt actcgacgct gaatcgctac gatcccacct
     1921 cttttgctga ttctcaggca attattgaat ttcggcaaaa attagcttca gaaatcaaca
     1981 agctgcagca tgccgttgct gaaggtcagt gctggcaaag cgactggcgg atcagtgaaa
     2041 gtgaagcggt ggcggcacgg gaatgtaacc tggagaatat ctgccagacg ttgttacaac
     2101 tgggccagat gaacccgaat acgccgccaa cgcccgccgc caaaccgcca tcaatggtcg
     2161 ccgatgcttt taccaatcca gactatatac gctacgcggt aaaaacattg ctcgcctgtt
     2221 tgatctgtta caccttttac agcggcgtgg actgggaagg cattcacacc tgtatgctga
     2281 cctgcgtgat cgtcgctaat ccaaatgtcg gttcgtcgta ccagaagatg gtgctgcgtt
     2341 ttggcggggc cttttgcggc gcgattctgg cgctgttatt cacgctactg gtcatgccct
     2401 ggctggacaa tattgtcgaa ttgctgtttg tgctggcacc gattttcctg ttggccgcat
     2461 ggattgccac cagctctgaa cgctcttctt atatcggcac acagatggtg gtcaccttcg
     2521 cgctcgccac gctcgaaaac gtttttggcc cagtgtacga cctggtggaa attcgcgatc
     2581 gcgccctggg tatcatcatt ggtaccgtgg tgtccgcgat gatttacacc tttgtctggc
     2641 ctgaaagtga agcgcgcacg ctgccgcaaa aactggctgg cgcgctgggt atgttaagta
     2701 aagtaatgcg gatcccacgc cagcaggaag tcacggctct gcgcacttat ctgcaaattc
     2761 gtatcggtct gcatgcggcg tttaatgcct gtgaagagat gtgccaacgc gtggcgctgg
     2821 agcgtcaact ggacagcgaa gaacgcgcct tactgattga acgttcgcaa acggttattc
     2881 gtcagggccg cgatatcctt cacgcctggg atgccacctg gaattcggcg caggcgctgg
     2941 ataacgcact acagccggac agagcaggtc agtttgccga cgccctggag aaatacgctg
     3001 ccggtctggc aaccgcactc agccgttctc ctcaaataac gcttgaagag acacccacct
     3061 cgcaggccat cctgcccacc ttattaaaac aggagcaaca cgtctgccag cttttcgccc
     3121 gcttgccaga ctggacagcc ccggcattaa cgcccgccac ggaacaggca caaggagcca
     3181 cgcaatgatc aatcgtcaac tttcacgtct gctgttgtgc agcattctcg gcagcacgac
     3241 gctgatttcc ggctgtgccc tggtacgtaa ggattctgcg cctcatcaac agctcaaacc
     3301 ggaacaaatc aaactggccg atgatattca tcttgccagc tccggctggc cgcaggcgca
     3361 gtggtggaaa caactcaatg acccgcagtt ggatgccctg atccaacgga cgctaagtgg
     3421 ttcacacacc ctcgccgaag cgaaactgcg ggaagaaaaa gcgcaatcgc aggccgattt
     3481 gttagatgcc ggttcacagt tgcaggtggc agcgttaggg atgcttaacc gccaacgtgt
     3541 ctcggcgaac ggctttttaa gcccttatgc gatggatgcg cccgcactgg ggatggacgg
     3601 gccgtactat acggaagcca cagtaggttt gtttgccgga ctggatcttg atttgtgggg
     3661 tgtgcatcgc tcagcggttg ccgccgccat tggcgcgcat aatgccgcgc tggcagaaac
     3721 cgcagcagta gagctatcgc tgaccacggg cgtagcgcag ctttattaca gtatgcaggc
     3781 cagctatcag atgctcgatc tgttagaaca aactcgcgat gtgattgatt acgcggtgaa
     3841 agcgcaccaa agtaaagtgg cgcacggtct ggaagcacaa gtgcctttcc acggcgcgcg
     3901 ggcacagatt ctggcggtcg ataaacaaat tgctgccgtc aaagggcaaa tcaccgaaac
     3961 gagagaatct ctgcgcgcat tgattggcgc gggagccagc gatatgccgg agatcaaacc
     4021 tgtggcatta ccacgagtcc agaccggcat tccggcgaca ctctcttatg agttgctcgc
     4081 cagacgcccg gatctgcaag ccatgcgctg gtatgttcag gcgtcattag atcaggtgga
     4141 ttccgcgcgg gcgttgttct atccgagctt tgatatcaaa gcgtttttcg gccttgatgc
     4201 catccacctg gataccttat tcaaaaaaac cagtcgccag ttcaacttca tcccgggtct
     4261 gaaattgccg ctgtttgacg gtggacggtt gaatgccaat ctcgaaggca cgcgcgccgc
     4321 cagcaacatg atgattgaac gttacaacca gtcagtactg aacgcggtgc gtgacgttgc
     4381 cgtcaacggc acgcgtctgc aaacgctcaa cgacgagcga gaaatgcagg ctgaacacgt
     4441 ggaagcaacg cgcttcaccc agcgcgctgc cgaggccgcc tatcagcgcg gcttaaccag
     4501 ccgcttacag gccactgaag cccggttgcc agtacttgcc gaagagatgt cattactgat
     4561 gctggacagc cgccgggtga tccaaagcat tcagttgatg aaatcgctgg gcggcgggta
     4621 tcaggcggct cccgtcgtcg agaaaaaata aaatgtctgc cgcgtgatgg ctgtcacgcg
     4681 gtatttcgtt tcgtcacgtc aaaactgacg acagcctgtt tttcgtcaga gttttgaata
     4741 aatagtgccc gtaatatcag ggaatgaccc caaataaaat gtggcataaa agatgcatac
     4801 tgtagtcgag agcgcgtatg cgtgatttga ttaactggag cgagaccgat gaaaaaagtc
     4861 gtcacggttt gcccctattg cgcatcaggt tgcaaaatca acctggtcgt cgataacggc
     4921 aaaatcgtcc gggcggaggc agcgcagggg aaaaccaacc agggtaccct gtgtctgaag
     4981 ggttattatg gctgggattt cattaacgat acccagatcc tgaccccgcg cctgaaaacc
     5041 cccatgatcc gtcgccagcg tggcggcaaa ctcgaacctg tttcctgaga tgaggcactg
     5101 aattacgttg ccgagcgcct gagcgccatc aaagagaagt acggtccgga tgccatccag
     5161 acgaccggct cctcgcgtgg tacgggtaac gaaaccaact atgtaatgca aaaatttgcg
     5221 cgcgccgtta ttggtaccaa taacgttgac tgctgcgctc gtgtctgaca cggcccatcg
     5281 gttgcaggtc tgcaccaatc ggtcggtaat ggcgcaatga gcaatgctat taacgaaatt
     5341 gataataccg atttagtgtt cgttttcggg tacaacccgg cggattccca cccaatcgtg
     5401 gcgaatcacg taattaacgc taaacgtaac ggggcgaaaa ttatcgtctg cgatccgcgc
     5461 aaaattgaaa ccgcgcgcat tgctgacatg cacattgcac tgaaaaacgg ctcgaacatc
     5521 gcgctgttga atgcgatggg ccatgtcatt attgaagaaa atctgtacga caaagcgttc
     5581 gtcgcttccc gtacagaagg ctttgaagag tatcgtaaaa tcgttgaagg ctacacgccg
     5641 gagtcggttg aagatatcac cggtgtcagc gccagtgaga ttcgtcaggc ggcacggatg
     5701 tatgcccagg cgaaaagcgc cgccatcctg tggggcatgg gtgtaaccca gttctaccag
     5761 ggcgtggaaa ccgtgcgttc tctgaccagc ctcgcgatgc tgaccagtaa cctcggtaag
     5821 ccgcatgcgg gtgtaaaccc ggttcgtggt cagaacaacg tgcagggtgc ctgcgatatg
     5881 ggcgcgctgc cggatacgta tccgggatac cagtacgtga aagatccggc taaccgcgaa
     5941 aaattcgcca aagcctgggg cgtggaaagc ctgcctgctc ataccggcta tcgcatcagc
     6001 gagctgccgc accgcgcagc gcatggcgaa gtgcgtgccg cgtacattat gggcgaagat
     6061 ccgctgcaaa ctgatgcgga gctgtcggca gtacgtaaag cctttgaaga tctggaactg
     6121 gtcatcgttc aggacatctt tataaccaaa accgcgtcgg cggcggatgt cattttgccg
     6181 tcaacgtcgt ggggcgagca tgaaggcgtg tttactgcgg ctgaccgtgg cttccagcgt
     6241 ttcttcaaag cagttgaacc gaaatgggat ctgaaaacgg actggcaaat catcagtgaa
     6301 atcgccaccc gtatgggtta tccgatgcac tacaacaaca cccaggagat ctgggatgag
     6361 ttgcgtcatc tgtgcccgga tttctacggt gcgacttacg agaaaatggg cgaactgggc
     6421 ttcattcagt ggccttgccg cgatacttca gatgccgatc aggggacttc ttatctgttt
     6481 aaagagaagt ttgatacccc gaacggtctg gcgcagttct tcacctgcga ctgggtagcg
     6541 ccaatcgaca aactcaccga cgagtacccg atggtactgt caacggtgcg tgaagttggt
     6601 cactactctt gccgttcgat gaccggtaac tgtgcggcac tggcggcgct ggctgatgaa
     6661 cctggctacg cacaaatcaa taccgaagac gccaaacgtc tgggtattga agatgaggca
     6721 ttggtttgag tgcactcgcg taaaggcaaa attatcaccc gtgcgcaggt cagcgatcgt
     6781 ccgaacaaag gggcgattta catgacctac cagtggtgga ttggtgcctg taacgagctg
     6841 gttaccgaaa gcttaagccc gattacgaaa acgccggaat acaaatactg cgccgtgcgc
     6901 gtcgagccga tctccgatca gcgcgccgcc gagcagtacg tgattgacga gtacaacaag
     6961 ttgaaaactc gcctgcgcga agcggcactg gcgtaataca gtcctttcta cagcctcctt
     7021 tcggaggctg tttttttatc cattcgaact ctttatactg gttactccct acccaatcgt
     7081 attatcaaaa tgaaaaaaat tatcgcattg atgttgtttt tgacattctt tgcccacgcg
     7141 aacgactccg agcctggcag ccagtattta aaggcagcag aggccgggga ccgacgtgca
     7201 caatattttc ttgccgacag ctggtttagc tccggcgatt tgagcaaagc cgaatattgg
     7261 gcacagaaag ccgccgacag cggtgatgct gatgcctgcg cgctgttggc gcagatcaaa
     7321 atcaccaatc cggtcagtct ggactatcca caagcaaaag ttcttgcaga gaaagcggcg
     7381 caagcgggca gtaaagaagg tgaagtaacg ctggcgcata ttctggtaaa tactcaagcg
     7441 ggtaaaccgg attatccaaa ggcgatttcg ctgttagaaa acgcctcgga agatctggag
     7501 aatgactccg ccgtcgatgc ccaaatgctg cttggtttga tttacgccaa cggcgtgggc
     7561 attaaggccg acgatgacaa ggcaacctgg tatttcaaac gcagctctgc aatttcccga
     7621 accggttatt ccgagtactg ggcgggaatg atgttcttaa acggtgagga aggttttatc
     7681 gagaagaaca aacaaaaggc gctgcactgg ttgaacctga gctgtatgga agggtttgat
     7741 accgggtgtg aagagtttga aaaattaacc aacggttaag gtaggcctga taagacgcgg
     7801 caagcgtcgc atcaggcatc cacacattgc cgggggatat ccccggcaat cttcaattat
     7861 tgatccgcag ttttatcaaa tttgcccagc acttcacgct cataagccag cgcctttttg
     7921 cggtcaaatt tgtgttccca cttggcaatc accagcaccg ccagcgcatt acccaccacg
     7981 ttcagcgcag tacgcgccat gtcgaggata cggtcaacac cagcaataaa cgccagacct
     8041 tccagcggaa tacctacgct acccagcgtt gccagcaaca ccacaaacga cacgccagcc
     8101 acgccagcaa tccctttcga ggtcaccatc agcgtcagca ccagaatgat ttcctgccag
     8161 atggacaggt caatgccata cagctgagcg atgaaaatag cggcaatact ttgatacagc
     8221 gtcgaaccat caaggttaaa agagtaaccg gtcggcacca cgaagctggt gatcgacgcc
     8281 ggtgctccgt aggcttccat cttctcaata attcgcggca gtacgctttc agagctggca
     8341 gtggagtacg ccagaatcag ctcatctttc agaatacgaa tcaggatcca gacgcttaac
     8401 ccgcacagac gtgccacaat tcccagcact accagcgcga agaacagaat ggcgaaatgc
     8461 accagcagca ccagtttcgc cagtggccac agagacgaga aaccaaagtt agccaccgtc
     8521 accgcaatca gcgcaaacac acccaccggt gcataacgca tcaccatgtg agtcacttta
     8581 aacatggttt cagagatgga gcggaacacg gtcaccagcg gttcacgatg cgtcgcgggc
     8641 agggaagaaa gccccagacc aaacagcacc gagaaaaaga tgatcggcag catttcgcct
     8701 ttcgccatcg acgccacaat gttcgtcggc accagcgaca aaatcgtgcc cataatgccg
     8761 tgggaactgc tttgtaccgc ttccgtagtg ctctgatatt tcgagatatc gacggtcgcc
     8821 agctgcgaca tatccacccc ggcaccgggc tggaagacgt tcgccagagt gatccccaaa
     8881 atgatggcga cggtggtgat cacctcgaag taaataatgg ttttcgcgcc aatacgcccg
     8941 agctgtttgg catcaccaac acccgcgata cccaccacca gcgtggagat cacaatcggc
     9001 acaacaatca ttttgatcag atggatgaag atatcacccg ccggagagag caaattgacg
     9061 accagccagt cgcggctgtc gctatggtag tgcaggtagc ttcccaggag aatgcccagc
     9121 accatagcaa acagaatctg ccaggccagg ctgaatttta tatttttcat cagaataact
     9181 tcctcaatgg agcgccgttc cccgttcaga aacgcgagaa cgtgacatac tgccagggca
     9241 tgaattgtgt tgcgttggtt tgtagatttt tttaacgctt atgatcagta tcatttgcgc
     9301 ggtatgttgc gcaacccttt aacaacagag cttttcgctg cattaatgca taataacgcg
     9361 agtttatggc aggattaatg aataaccatt tattatggaa agatttttat ttgtttaatc
     9421 tgcaataata ctgattggtg attatttttg gcatatgttc attcgattat tttttatgca
     9481 gctcaatgac ataatcttca tcaccagatg attaatccga tctccgctgc aacagttttg
     9541 ccatattaat cgactcgacc agctgtgtcc ggttgatccg gggtgagttg agatccatca
     9601 ctttttgcca tagttcgatg gcttgcgcgt aattcgcctg cataaacgca tcggaagcca
     9661 gcagcatcag agcggtgatt tcgttagagt ccagcgcgag ggctttgtcg atcattgcgc
     9721 gagtctgggc ggtcatatgc tggctggcct ggtaataaag caccgtcgcc agcgccgcat
     9781 acagttcagc gttctcacca cgtagttgca acgcctgacg atacgccagc agcgaattgc
     9841 tgtaatcgtt ttgccacaga tagtactcgc ccagtaacgc ccattgttcg ctgttttgtg
     9901 gattagcgcg gattttatcc tgcaatgcct gaagctgcgc ttccggggtt tgctggctgg
     9961 caaactgatg tagcggatcg cgctgacgct gatactccgc acgtaccgcc tgccattttg
    10021 ggcttaacag ataactgccg acacagagga ataccatcaa tatcgtgagt gttgtcagca
    10081 ttttcaccgg tatttgaggc tgtttcatcg cttagccctc actcgccaga ggatcagtgc
    10141 catcagcaat aacaacacca ctggcagcgc ccacaacacc agcgtctgac ccgttaacgg
    10201 tgggttatag cgaacaaaat ctccgtagcg ttcggtcatc cagccaatga tttcgacttc
    10261 gttcttcccc tccgacacca tgctataaac ctgatggcgc atactgaccg ccaccggcgc
    10321 attggattcg agtaagtttt gattctggca ctgcggacaa cgtaactggc tggcaatatt
    10381 taacgcctgt tgctgttgtt gcggattggc gaattgccag gtgtctacaa cctgagcgcg
    10441 cgcaaaacag gtaaataaca gcagtagcgt gagaagccct ttattcatcg cgcttcttcc
    10501 cccgccatcc gcttaacaat gcgcccgcaa tcatcaacaa tcctcccccc cagatccagc
    10561 gcacaccgct ttgtacatac aaacgaaaag cgtaacgatc cgctctggtt ttttcaccca
    10621 tgaccgcata ccagtcatgg atgccgttcc agcgaattga cggttccatc atttgctgac
    10681 ggcgtgcttc ataaaaacgc cgctccggcg ttaattcacc aatgcgttgc tgatggtcaa
    10741 acagcgccac tatcgctttt tcgctggtgt aattgcctct ggcttgcaga tcgagtcgct
    10801 caaaacggaa ggtgtatcct gccagcgtca cctgctgacc cggctgtaaa ttcaggctga
    10861 tctcctgacg actgacactg gaaaccacga tccccgcggc aaataacagc acgccagcat
    10921 gagctaccag cgccggaagc tgcacgcgtt tgccagagac aaacgtcgcc agcacaatca
    10981 ccaccagcat caacagacca aacggtaacg tcgcgcggtt aaaatacggc gcgccaacgg
    11041 agaggcgtcc ccagcccagc aggccgtaaa tcatcggata aagcgttccc accagcacga
    11101 tcagtagcac tgcgcaaaac agcaacagcg tagcgaggat taacatttcc cgcgataacc
    11161 ccgaaaaacg caccgccggg ccaccgtccc gcgctcgcca gccatacaga gccaaagacg
    11221 caaggctaat cagtgcaaac aggctgaaca acggcacggc gcggacgtta tccagtgcga
    11281 acgcatgaac cgaaaccaga atgccagaac ggacaattaa ggtgcccagc agcgacagca
    11341 tcagagtaac tatcgccagc aacagcgacc agtggcggaa aatcccccgc tggcgtgtca
    11401 gggacaaact gtgcagcagc gcagtggcag aaagccaggg taataaagag gcgttttcca
    11461 ccggatccca gaaccaccag ccgccccagc cgagttcgca gtaggcccac caggaaccga
    11521 ggatgatccc cgccgttaat gcactccagc caggtaacgc ccagcgccag caaattcggg
    11581 cgctggccgc atcaaactcg ccgcgcagta gactcgccag cgccacgctc gccgctacca
    11641 tcaaaccgcc atagccaagg caaagcagcg gtggatgaaa gataagaccg ggatgttgca
    11701 gcatcggatt gagatcgcgg ccttcgattg ctggtggaaa tatccgcacg aagggatcgg
    11761 accacagcac cacaaacagt agcagtgcgg cgagcatgag agataaaacg gctagcgtca
    11821 gcggaaatag cggatcgctt tgctgccgat aatgccaggc aaacagcgcg ctccaggcgg
    11881 aaagcagcag cacccaaagc agcagcgaac cttcatgacc gccccacacc gccgccagtt
    11941 tgagttccca cgacagcagg ctgtagctat gttgggcgac ataaatgacc gagaaatcgc
    12001 tgatgagaaa acaatacgtc agtacgccaa aggcgagcag caggagcgcg aactgcgcca
    12061 gaatgccgat gcaagtgagt cgcatcatgg caggccagcg caaccgcact cccgcaaagg
    12121 ccgtcaacgg ggtcaacacg ttgaccccga gacttaacaa caacgccaga aaaccgactt
    12181 caggaaggaa tacgtccaga aaccacctct tttctggctt acgccaccgt caactgtccg
    12241 gcgtagagaa tgaaaaagcg cagcatcaac acgccgacca gactggcccc acacgccgcc
    12301 agcacggcag gaatgccgga actgcgattg acccacggtt tgagcaacat tggcacaatc
    12361 agccccagcc cggcgacacc aagccagaac caccacgtcc agaaaccgcc acctaatgcc
    12421 gccaccagcg cacgaacttt accgtcatcg cccagcgcca gaccgacaaa aaacgccacc
    12481 agcaggaaga tttcacccca taccacgggg atttccatcc ggtgaacaaa gtgcgcttcc
    12541 gtggaatgcg ggttactgcg ttggcgtatc gccatggcga tcagcgccac cgccgcaccg
    12601 gacgagatgc cggagaagag gagcagcacc ggcaggctcg ggttattgag gaacggatac
    12661 gatttcagcg ccgacagcag aaagccggta taagccccca gcagcaccgc caacaccagc
    12721 atcagcgttt ccagtccgcg atgaacgggc gtcagcaggc tcagaacctt ttgcacgatc
    12781 cccagcttcg gcaaccagcg ttgttgcagg gcaagcaaat catgttcaaa gattttcgcc
    12841 agccacagca ccagcaccac catgtagagc tgaaacagca tcacccccat cgacatcacc
    12901 gaggtaaaac tgtagtggaa catcagcttc cagaaggtcc acggtcttgt caggtggaag
    12961 acgaggatca acagaccgag gatcaccgcg cccggcccga caatcagcgt ggtgcgcagc
    13021 aacgtactgt ctgcaccgcc cgcctgcggg tagaagcgac gtaacagcac ggccagcgtc
    13081 accagaccgg cagaaatacc aatcaaaaac aggtagatgg caatcggcca gtcccacacc
    13141 agcgattcaa aatgaaatgc ggaagtctgc gtcattggct cacctccccg tatttaaacg
    13201 gtacgcggta cagcttcggt ttggttccca gcgccagctt gtagcggtaa gtaggcttct
    13261 ggcgcagcag ttgcgaaatc tcactgttag gatcgtccag attgccaaac gtcagcgcct
    13321 tcgtcgggca agcttcaacg cacgcgggaa gcttaccggc ctgcaaattg gtcttacgac
    13381 agaaatcgca tttgtccgcc gtcttcgtga ccggatggat aaagcgcacg cggtacggac
    13441 aggcggcgat gcagtactga caaccgacgc agagatcagg attaacatcg acaatcccgc
    13501 tagcggcatc acgaaaagac gcaccggtcg ggcagacgtc aacgcacggc acatgatcgc
    13561 agtgctggca agacttacgg aagaagcgat atttcacatc aggaaattcg ccctgcggct
    13621 cgctacgaat aatcgtcagg cgcgagacgc cttccggcac cttgttcact tcccgacaag
    13681 catccataca ggcggtacag ccgatgcata acgactcgtc atgcaccatg ccgtaacgca
    13741 ccccattgat attcaacgtt ttcgccacca cgcgccctgc ggtgccgctg acggctgcca
    13801 gcacgccgac gccggtgaga aactggcgac gagaccaggt catggctgct ccttaagcaa
    13861 cggaacagac gccgggttaa agttcggatt ggtgcgctga tcgctgtggc aatcgacgca
    13921 aatcttaatc cgtcctttgt cacttaacgt ctgcatcgta tcttgctgtg gatgcaggga
    13981 atggcagctg gcgcacgcca ctttggttac gtggacatcg tgcggccaga acgctttttg
    14041 caactgttcc ggcagatgac aggacataca gacgctgttc tgctctccga ccttatacat
    14101 cggctcgtta aagcgcatca catctttcac cccttcgcgg tgttgcggtg atgcttggcc
    14161 gtgacagttg gtgcaggtga ccggcaattt gttattcggg ttgataacgg aagcatgttt
    14221 gccatgcata ccttcggtat ctggtttatg acagtccaga caggcggcat ccgggttacg
    14281 ctgctgggta acttcgtagc gatcaccaga tgcctgcacc gcaggggtag cggtaattcc
    14341 gttcaggctc cacaacaggc ccgacgccag caccccagca gttaataacg aacgtaatac
    14401 gctcatattc actccattgt gttttccgcc ccttgcgagg cggaacgggg ttattggctt
    14461 aacagaccgt ttttacgtgc ctgctcttcc cactgcggga tcaccgtttt gatgaagtcc
    14521 tgcttctcgg ccttgatttg ttccatgttc aggccaatgg cctgctgggc tttctctttg
    14581 gtcgagatat ccgggatctg gatttcatgg gtgatacctt tggtcgccag caggcgcgcc
    14641 agtttggtgc gcgcatccgc cgctttatcc atcgccgtac cgagcatccg tagaccttct
    14701 tccggtgcgt gcatatgaat accgtgggaa gcgatcgcca gatcccaacg ccactgggca
    14761 tgacggatat cgtcctgaat cggcttcatt tcggcttccg tcgcgcctgc atccagcgcc
    14821 gctttcgctt cgaagtgagc gtgaaccagt tgatcttcaa ccttgatttt caggtcgtta
    14881 atcgattgct tacgttccgc gaccactttt tgcaaggcag ctttatcctg ggtatggcag
    14941 ttcgcacaag tctgggcgaa gttatcaaac ggattaccaa ttttatggtc ggtgtagagt
    15001 ttgccttcgg cgttctgcac ttttggcata tggcagtcga tacaggtcac gttgttttta
    15061 ccgtgaatgc ccgctgtcca ggtttcatat tccgggtgct gcgctttcag cattggcgtt
    15121 ttcgacaggg agttagtcca gtcagagaag gcaattttgt cgtaatactg ctccatattt
    15181 tcgactttca tgccgtcatc ccacgggaat ttaaccgctt tgtttttgcc gtcgaagtaa
    15241 tactccacat ggcactgacc gcaaaccatc gattgctggt cgaaacgtcc ggctttctca
    15301 aaaggtttac caatggcttc catcgcgcga gccgcatacg gacgggaaag ggttaactcc
    15361 ggtttgcctt tggcgaactc tggtgaggcg gtgttatggc agtcggcaca acctaagttg
    15421 ttgacgattt ccgggccgcc gcgcgcccat ttaccgtgga agtagccatc ttcgccgtct
    15481 ttctggatca gacgcgccac atccgggctt ttacaactcc agcacgccat cggtagcgga
    15541 ccatcttcag cgtttttcgg cgcaccggta cgcagggttt cacgcacatc ggtcacagca
    15601 aaggcatgtc cacgaggctt gttgtaatcg cgcgagaagg gataccccgc ccacaggatc
    15661 accagccgtg gatcttccgc cagggcgtca acacgctctg actgttccga ggtggctttc
    15721 caggagagat attgatcgtg atgctgcggg gcaaaggttt cattcttcgc ttccacagtt
    15781 acaggttttg cgggagctgc cgtttgttca gcgtgaacag aagtgaaaaa gaaaaaagga
    15841 atcaataagc tgaagatacg gcgtgcgttt atttttatcc ttgtcatagg ggcttcatcc
    15901 gaattgcgcc attgttgcaa tggcggtttt tattgttttt cacgacagta accgcaccta
    15961 cactgtcatg acattgctca cccctatgtg taacaaataa ccacactgtg aatgttgtct
    16021 ttaatcaatt gtaagtgcat gtgaaatacc actttagagt tagtcagtat cttcctcttt
    16081 ttcaacagca tgcataactg catgttcctc aaagaattaa tcaacttttg ttggtgacct
    16141 tcaaaaatta ccctgccgtt tatttgcacc attctacttt tgcgtgatct gtcgcccaaa
    16201 tactaaacaa aactgccaat acccctacat ttaacgctta tgccacatat tattaacatc
    16261 ctacaaggag aacaaaagca tgagccaaat tcacaaacac accattcctg ccaacatcgc
    16321 agaccgttgc ctgataaacc ctcagcagta cgaggcgatg tatcaacaat ctattaacgt
    16381 acctgatacc ttctggggcg aacagggaaa aattctcgac tggatcaaac cgtaccagaa
    16441 ggtgaaaaat acctcctttg cccccggtaa tgtgtccatt aaatggtacg aggacggcac
    16501 gctgaatctg gcggcaaact gccttgaccg ccatctgcaa gaaaacggcg atcgcaccgc
    16561 catcatctgg gaaggcgacg acgccagcca gagcaaacat atcagctata aagagctgca
    16621 ccgcgacgtc tgccgcttcg ccaataccct gctcgagctg ggcattaaaa aaggtgatgt
    16681 ggtggcgatt tatatgccga tggtgccgga agccgcggtt gcgatgctgg cctgcgcccg
    16741 cattggcgcg atgcattcgg tgattttcgg cggcttctcg ccggaagccg ttgccgggcg
    16801 cattattgat tccaactcac gactggtgat cacttccgac gaaggcgtgc gcgccgggcg
    16861 tagtattccg ctgaagaaaa acgttgatga cgcactaaaa aacccgaacg tcaccagcgt
    16921 agagcatgtg gtggtactga agcgtactgg cggaaacatc gactggcagg aagggcgcga
    16981 cctgtggtgg cacgacctgg ttgagcaagc cagcgatcag caccaggcgg aagagatgaa
    17041 cgccgaagat ccgctgttta ttctctatac ctccggttct accggaaaac caaaaggcgt
    17101 actgcacact accggcggtt atctggtgta cgcggcgctg acctttaaat atgtctttga
    17161 ttatcatccg ggtgatatct actggtgcac cgccgatgtg ggctgggtta ccggacacag
    17221 ttatttgctg tacggcccgc tggcctgcgg cgcgaccacg ctgatgtttg aaggcgtacc
    17281 gaactggccg acgcctgccc gtatggcgca ggtggtggac aagcatcagg tcaatattct
    17341 ctataccgca cccacggcga tccgcgcgct gatggcggaa ggcgataaag cgattgaagg
    17401 caccgaccgt tcgtcgctac gcattctcgg ttccgtgggc gagccaatta acccggaagc
    17461 gtgggagtgg tactggaaaa aaatcggcaa cgagaaatgt ccggtggtcg atacctggtg
    17521 gcagaccgaa accggcggtt tcatgatcac cccgctgcct ggcgctaccg agctgaaagc
    17581 cggttcggca acacgtccgt tcttcggcgt gcaaccggcg ctggtcgata acgaaggtaa
    17641 cccgctggaa ggggctaccg aaggtagcct ggtgatcacc gactcctggc ctggtcaggc
    17701 gcgtacgctg tttggcgatc acgaacgttt tgagcagacc tatttttcca ccttcaaaaa
    17761 tatgtatttc agcggcgacg gcgcgcgtcg cgatgaagat ggctattact ggatcaccgg
    17821 gcgtgtggac gacgtgctga acgtctccgg tcaccgtctg gggacggcag agatagagtc
    17881 ggcgctggtg gcgcatccga agattgccga agccgccgta gtaggtattc cgcacaatat
    17941 taaaggtcag gcgatctacg cctatgtcac gcttaatcac ggggaggaac cgtcaccaga
    18001 actgtacgca gaagtccgca actgggtgcg taaagagatt ggcccgctgg cgacgccaga
    18061 cgtgctgcac tggaccgact ccctgcctaa aacccgctcc ggcaaaatta tgcgccgtat
    18121 tctgcgcaaa attgcggcgg gcgataccag caacctgggc gatacctcga cgcttgccga
    18181 tcctggcgtg gtcgagaagc tgcttgaaga gaagcaggct atcgcgatgc catcgtaacc
    18241 cacaattgcc ggatgcgaat cggtaacggt ttgtaggcct gataagacgc gacagcgtcg
    18301 catcaggcat tgattgccgg atgcggcgta aacgccttat ccggcctaca ttcggcaagg
    18361 gttacccgag cgttaacctt ctcccataag ggagcgggaa ttaaaacaat ccctacatta
    18421 cctctggaga atctgtgatg aatggtacga tttatcagcg gatagaagac aatgcgcatt
    18481 tcagggagtt agtcgaaaaa cggcaacggt ttgccaccat cctgtcgatt attatgctgg
    18541 cagtttatat cggctttatt ttactgatcg ccttcgcgcc cggctggctg ggcacgccgc
    18601 tgaatccgaa caccagcgtc acacgcggta ttccaattgg tgttggagtg attgtgatct
    18661 cctttgttct caccggtatc tacatctggc gggcgaacgg cgaattcgac cgtcttaata
    18721 atgaagtcct gcatgaggta caagcatcat gaaaagagtt ctgacggcgc ttgccgccac
    18781 actccctttc gcagctaacg ccgcggatgc tattagcggg gccgtagagc gccagccaac
    18841 gaactggcag gcgattatta tgttcctgat tttcgtcgtg tttacgctcg gcattaccta
    18901 ctgggcatca aaacgcgtac gttctcgtag cgactactac accgcaggcg gcaatatcac
    18961 tggcttccag aacgggctgg cgattgccgg ggactatatg tccgccgcct cattcttggg
    19021 gatctccgcg ctggtgttta cctccggcta tgacgggctg atctactcgt tgggcttcct
    19081 ggtgggctgg ccaatcattc tgtttctgat tgccgaacgt ctgcgtaacc tgggacgcta
    19141 cacctctgcc gatgtggcct cttatcgtct gaaacaaggg ccgattcgta ttctttcggc
    19201 ctgtggttct ctggtagtgg tggcgcttta ccttatcgct cagatggtag gcgcaggtaa
    19261 actgatcgag ctgctgtttg gccttaacta tcacattgcg gtggtgctgg tcggcgtgct
    19321 gatgatgatg tacgtcctgt tcggcggcat gctggcgacc acctgggtac aaattatcaa
    19381 agctgtgctg ttgctgttcg gtgccagctt tatggccttt atggtgatga aacacgtcgg
    19441 ctttagcttc aacaatctgt tcagcgaagc gatggcggta cacccgaaag gtgtcgacat
    19501 catgaaaccg ggcggactgg tgaaagatcc gatctccgcg ctctctctgg ggctgggact
    19561 gatgtttggt acggcgggct tgccgcacat tctgatgcgc ttctttacag tcagcgatgc
    19621 ccgcgaagca cgtaagagcg tgttctacgc caccggattt atgggctact tctatattct
    19681 gacctttatt atcggcttcg gcgcgatcat gcttgttggt gcgaatccgg aatataaaga
    19741 cgcggcgggc catctgattg gtggtaacaa catggcggcc gttcacctgg cgaatgcagt
    19801 gggcggcaac ctattcctcg gttttatttc agcggttgct ttcgccacta tcctcgcggt
    19861 ggttgcggat ctgacactgg cgggcgcatc ggcggtttcg catgacttgt acgctaacgt
    19921 cttcaaaaaa ggcgcgaccg aacgtgaaga gctgcgggta tcaaaaatca ccgtactgat
    19981 cctcggcgtg attgcgatta tcctcggcgt gctgtttgag aatcagaaca tcgcctttat
    20041 ggtaggcctt gcgtttgcca tcgcggcgag ctgtaacttc ccgatcattc tgctttccat
    20101 gtactggtcg aaactgacta cccgtggcgc gatgctgggc ggctggctgg ggctgattac
    20161 cgcagtggtg ctgatgatcc tcggcccgac tatttgggta cagatcctcg gtcacgaaaa
    20221 agccatcttc ccgtatgaat acccggcgct gttctctatc agcgtggcat tcctcggcat
    20281 ctggttcttc tcggcaaccg ataactcagc ggaaggggcg cgcgagcgtg aactgttccg
    20341 cgcgcagttt atccgctccc agaccggctt tggcgttgag caaggccgcg cacattaatc
    20401 tctccccttc cccggtcgcc tgaccgggga atacgcttcc tctccaacat gcatcacctt
    20461 ttcccataat attaaacaaa taaactcatt aaaaaatgag cgatttttga cagtcgtaga
    20521 aaatgataat gcagagaata tgccttttct ttcttgttaa ttataaggat attttatgcg
    20581 ctataatggt ttaaataata tgtttttccc tctttgccag attaacgata accactctat
    20641 cacaagttca tcacatacaa agaaaacaaa atcagataat tacagcaaac atcataaaaa
    20701 cacgttaatt gacaataaag ccctctctct tttcaaaata gatgatcatg aaaaagtgat
    20761 aggcttgatt cagaaaatga aaagaattta tgatagttta ccatcaggaa aaatcacgaa
    20821 agaaacggac aggaaaatac ataaatattt tatagatata gcgttatatg caaataataa
    20881 atgtgacgat agaattacga gaagagttta ccttagtaaa gaaaaggatg tatccattaa
    20941 ggtggtatat tttataaata atgtcgccgt ccataataat actatcgaaa ttccacagat
    21001 agtaaatggt ggttacgatt tttcacacct tagcctgaag ggtatcgtga ttaaagatga
    21061 agatttatcc aattcgaatt ttgcaggttg cagactacaa aacgctattt tccaggactg
    21121 taatatgtat aaaacgaatt tttattacgc cataatggaa aaaatacttt ttgataattg
    21181 tattctcgat gactcaaatt tcgctcagat aaaaatggcc gacggaactc taaatgcatg
    21241 ctccgctatg catgttcaat tctacaatgc agcaatgaat agagccaata ttaaaaatac
    21301 cttccttgac tattcaaatt tttatatggc gtacatggct gaggtaaatc tttataaagt
    21361 aatagcgcca tatgttaatt tatttaaagc cgaccttagt ttctctaaac tcgatttaat
    21421 taactttgaa catgctgatc tgtctcgcgt caatctgaac aaagcaatcc tccagaatat
    21481 aaacttaatt gatagcaaac tcttttgtac gtggctgaca aatacattcc tcgaaatggt
    21541 tatatgtacc ggctctaata tggctaatgt taattttaat aatgccaatt taagcaactg
    21601 ccatttcaac tgttctattt taacaaaagc ctgtatgttt aatacccgtc tctatcgggt
    21661 taatttcgat gaggctagcg tccagggaat gggcatttcc attctccgtg gggaggaaaa
    21721 tatccccatt gatagtgata ccctggtaac actacagaaa ttctttgaag aagattgtac
    21781 ctctcatact ggcatgtcac aaactgagga taatattaat gcagtcgcta tgaagattac
    21841 tgcagatatt atgcaacacg cagattgagg ttaagtcgaa gaacagccaa tgaattgact
    21901 ttacaagaga gataacttat ttgttttaga atcatgaatg ttcgcccgag ctaacctgtt
    21961 aaaggtgcaa aagattgcac ctttttcaga ttacctcggg cctggttacg tcgctttcgg
    22021 cttactgatt ttcctcaatt agcaacgctt caagcaaatc gagatcgtgc agtaattttt
    22081 gcagcgtttc gttgctgatc tcccgcgtgg cgcgcaggtg gtacagttca gcacgttcag
    22141 aacgcaatgc cgccagacgg aagcgacgct caaggttctc ttcctgcacg gaactttcaa
    22201 cgtcgttacg tccatcggcg cgacgacgca ggttaccaat gacgcgagaa ctgacctccg
    22261 taagcaactg gttatcgata ttctcttcgg tatccgccgc cagacgctcc tccattttct
    22321 ggatcgccac aatcgccact tctgccgttg ccgctcgcgc aatacgttct tctttcaatt
    22381 gttgcgaatg gtccgcgact tcaatgtgtt gtagcagaat aggcaacatc accacgccga
    22441 caaacagcga gaagagaatg acgccagccg ccaggaacac cagctcatag cgcgccggga
    22501 agacgttacc atccggcaag agcagcggga ttgagagcac accggccaga gtgatcgccc
    22561 cacgcacccc agcgaaagac gcgatcagga tttctcgtgt ggtccacgaa ccaaactcca
    22621 tcggcttctt cttcaggaag cggttgctga actttttcat cgtccacagc cagccgaaac
    22681 ggaccagcat cagcgccgca tatatcagaa taatatcggt aaacagcatc cagatttcga
    22741 cgttagggtc gatttctgcc gccatcagcg acgtctccag aatacccggc agttgcagac
    22801 ctaacagcag gaacaccatg ccgttaaaga caaattccag catcgcccag gtgctgtttg
    22861 cgcgcaggcg cattgccagt ggcgcacggc gcatcacacc ggagcgggtg atggtcatcc
    22921 cggcggcaac cgcagcgagg atgcccgaaa cgccaatatg ttcggcaatc agataagaag
    22981 cgaatggcag caagaacagc agaacgatct gcgtcgcggg ttcatcaccg ccccagcggc
    23041 tgaggaatcg cagcgagcga ccatacagcc agctcaccac aaaaccggca agaataccgc
    23101 caatggctac tttcataaat tcgaccgtcg caccgccgac agtgaagatc atcgtcccca
    23161 tcgccactgc cacggcaaac ttcaacgaca ccaggccgga agcgtcattc atcaacgctt
    23221 cgccctgcaa aatgcccatg atttttttcg ggatgcgccc ttcgccaaca atcccggaga
    23281 gcgccacagc atcggtcgga gaaagtaccg ccgccagcgc aaaggcgggg atcagcggaa
    23341 tgcccggcac cacccagtaa ataaggaagc caatgccgac cacggtgacc accaccagcg
    23401 ccagcgcgag gccgaaaatc tctcgaccat gttcgagaaa ttcacgggtc ggcgttttcc
    23461 agccatcagc gaacagcaac ggcgggataa acaagactaa aaagagttca ggatcaaact
    23521 ccacatgcaa accaaacgtc ggccacgcca gtagcgcacc gatggcgatt tgcataagcg
    23581 gaagcgggat ctgaaagggc atgacacgag tgaccacccc ggacagcgag accacgaggg
    23641 tcattatcag tatggtgaag aagatttcca tgcgttccct gtttgcgatg ttttttatga
    23701 tctgagaaag acgttatgcc tgaattctca ttttttcaga atatcgcaaa agctcacaca
    23761 atgtgcggag aaaataaaaa cgggcagcca ttgccacccg ttttcagaat ccgatgaaag
    23821 attagatagc ccagccaccc gcatagaagg tcaccagcgc cacggcgata acgaccgtac
    23881 cgatgttcaa cttgcgccat tcaccggaaa ccagacgacc aatcaccaga gtcgcgaagc
    23941 cgatcatgat gcctgttacg atgttacagg tcagcacgat gaataccgcc gtaaccagac
    24001 ccgccatcgc atcaacaaaa tcagcaaagt cgattttcgc cacgttgctc agcatcagca
    24061 ggccaacgta catcagcgcc ggagccgttg cataccccgg aacgaggtaa gaaagcggag
    24121 agaggaacag aatcagcagg aacagcacgc caacggtgat agccgtcaac ccggttttac
    24181 cgcccgccgc cgtacccgcc gcagactcga tgtataccgc tgccggagct gcacccacca
    24241 ggccagagaa aacgctgctc atggagtcag tggtcagtgc tttcccgccg tcgatgatct
    24301 gcccatcttt atccagcagg ttcgcctggc cggcgacagc gcggatagtt ccggtggcat
    24361 caaatactgc cgtcatcacc agcgccagaa cgcttggcag gactacagga ttcagcgcgc
    24421 ccataatgtc caggctgcca atcagtgaat tgccgttttc atcgctcaat gaaggcatgg
    24481 cgaaaacgcc ggagaaatgg acgttaggat cgaagatcaa accgacaatt gagataccga
    24541 taatggtcag cagaatgcca ccagggactt tcagtttttc caagccgatg atcaccgcca
    24601 gacctaccag tgacataatc accgggaagg tcgcgaaatc acccagcgca acgggcagac
    24661 catcaagcgg gtttttaatc accagaccga caccattagc ggcaatgagc agcaggaaca
    24721 gaccgatacc gatccccgtg ccgtgcgcca caccgtgcgg caagttgcgc aaaatccagc
    24781 tacggatacc cgtggcagaa attaccgtaa acagcacacc catcaggaac acggcaccca
    24841 gcgcgacagg tacgctaata tgttgcccca gcaccaggct gaatgcggta aacgccgtca
    24901 gggagatggc gcaaccaatc gccaacggca gattagccca cagacccatc acgatagaac
    24961 cgagtccggc aaccagacag gttgcaacga aaactgccgc aggcgggaag cctgctttac
    25021 ccaacatacc tggaacgacg atgaccgagt agaccatcgc cagaaacgtt gttaacccgg
    25081 caaccacttc ctgacggaca gtgcttccac gttgtgaaat tttaaaccag gcgtcgagtg
    25141 aaccgccggt acgcgctgat ggcgtagaca taagaaaaca tcccctgaga gtttaatttt
    25201 cgtcaagatg cgtggcggac aatcccctgc cagcgaaacg ttatcttcct gctccaggtt
    25261 tgcggcaatt tggtgcgtcg caaaaaagca aacgtttagc tccgcgcata caaaacgggt
    25321 ggtaaaaaaa agcaaacgat tatccggcga tcatagcggc tttttcaacc gaagtttgct
    25381 gcttttttct cttttattgt cttgacgatg aaaaaccagg caattgatgc gcaaacgtta
    25441 tcgtcgatgt gcaagattgc agcgttatcg agccgtatac cgttctggat tgcgataaaa
    25501 aatcgtctga gatgacaaaa atattgcggc taaaaagtat acttttacac tggcgaagaa
    25561 acgtcggtga catacaatta aagcatcaac accaaccgga acctccacca cgtgctcgaa
    25621 tgaggtgtgt tgacgtcggg ggaaaccctc ctgtgttcca gcgggataga gagaaagaca
    25681 aagaccggaa aacaaactaa agcgcccttg tggcgcttta gttttgtgtt tgcggataat
    25741 tccaaaagag ctcgatcagt cccctcgccc cttcggggag agggttaggg ttaggggaac
    25801 aggcagcacc agtgcaaata tttaccctca ccccggcccc atagttaacg atgagtcagc
    25861 ttgtccttgt ggcgctttag ttttgttcat cttccagcaa gcgtgcgccg gtaccttctt
    25921 ctcctaagcg gtcgcccggg ttacgcaacg ggcaatcact gcgcgaaagg cagccacaac
    25981 caatacatcc gtccagttcg tcacgcagcg ccactaaggt atgaatgcgc cgatccaact
    26041 cttctcgcca ttgggacgaa agctgtttcc actctttcgc acttaacgta tgcccttctg
    26101 gcaacacgcc aaacgcttca ccaatggtcg ccagcggaat gccaatacgc tgagcgattt
    26161 tgataattgc gacatatcgc aacacatcac gtttatatcg ccgctgattg ccgctgttac
    26221 ggatactagt aatcaaccct ttgctttcat agaaatgcag cgccgatacc gccacaccgc
    26281 tgcgtttcgc cacttcgcca ggggttagca gcgctttaat gcggggtaat ttcttttcca
    26341 taaatcgctt tacctcaagt taacttgagg aattatactc cccaacagat gaattaacga
    26401 actgaacact gaaaagaggc agatttatgt cccatcagaa aattattcag gatcttatcg
    26461 catggattga cgtgcatatt gaccagccgc ttaacattga tgtcgtcgca aaaaaatcag
    26521 gctattcaaa gtggtacttg caacggatgt tccgcacggt gacgcatcag acgcttggcg
    26581 attacattcg ccaacgccgc ctgttactgg ccgccgttga gttgcgcacc accgagcgtc
    26641 cgatttttga tatcgcaatg gacctgggtt atgtctcgca gcagaccttc tcccgcgttt
    26701 tccgtcggca gtttgatcgc actcccagcg attatcgcca ccgcctgtag ttttattgcc
    26761 cgcgcgttaa ctcccgcgcg ggtaattgct ccatccattg cataaacacc tgcggcgcca
    26821 tcgccttcgc aaaaaaccat ccctggcaat agcgcacgcc gcgtttgcgc agccagttaa
    26881 cctgctcctc agtttcgacg ccttcagcga tcgtttttaa ccccaggctg tgcgccagct
    26941 cgatgatgtg ttccgcaatc aaatgactgg ttttgtgggt ggtcagcgtt tcaacaaacg
    27001 atttatcgat tttcaaaata tcgacattca atgatttaag gttatgcaag ttagagtagc
    27061 caataccaaa atcatcaatt gccacttcgt aaccagcctg gcggaaagcc agaattaatc
    27121 ggcgtcattt tttcgacatc aagaaatgca tgctcagtca cttcaaattt aatttgctgc
    27181 ggacgcaccg cgtattgctc tgttttctga ttgattcgcg ctatcaaccg tgacgtatga
    27241 aaatcggagg ccgacaggtt aatagaaaca tagcgatcgg catgtgttgc caggtacgcg
    27301 cccagatcgc ggaagacatt atcaataaca taatcagtta tctgttctat catcccctcc
    27361 ttttctgcca gcggaataaa ctctgccgga ttcattattt gcccctgctc accaggccaa
    27421 cgtaacaacg cttcagcgcc gatacatttt tctgttttga tatcgattat tggctggtaa
    27481 taaagacaaa gttgatgttt ttcgagggcg cgttgcaatt tacgtttggg agataaatag
    27541 ttttgtcgaa tacgtagcca gagtaatagc agaacaagac tccccaaaat accggcgggc
    27601 aacgcaaaaa taagatgatt ataaaaatgg gttataagac gttgatatga agtcgcaaca
    27661 atggctgcaa ttgggcgttt tgtcgaataa attgtcgcat ataaatagcc atttctttgt
    27721 acggttaaat ccttcaaatg aatcagcgga gaaaacgttg ctgccgaggc ctctttgctt
    27781 aacgagaaaa aggttttcgt caccgtatca tacacacccc attgcaatgt cgggtcatca
    27841 gacatcactt cactccagaa gagagggttg ataaccgcca cataatttcc ccgctgcata
    27901 taggtcattt tatagccaga gaaaaaaggc gtatcgcggt aataatagat agaaacgtta
    27961 ggttcacgct tataatcggc cggtgcaatc gtatagccgt ttacaggcgc tatcagcgat
    28021 gagcataaaa aatggttatc acgaattcat taatataaag atagccacga ataatattca
    28081 acattcgctt ttgatgggct ggagtgcaaa cttgcccctg atagcgttca gctgcatcgc
    28141 tcactaaatc tgcctggcga atgaccagct cggatttatc cagtgccagt tgagcaaagg
    28201 ttcggagctg attattcact tctgattttg cccaaaggaa ggcaatccat agcgaaagaa
    28261 tgatgggaaa gagaactaaa aagataatgc ccggcaacgc cagtaattgg tgtcgtgcac
    28321 gatgactcat tgctatcaat ttcctgcctg tttataccca gccattgcaa ggtgaaaccc
    28381 tggctaccga cgtctttaga taaatcttac ggtataaata gcacttttgc ctgatttgaa
    28441 attgatacct ttttaatcgt cacctggatg aaaataattc attgatattc aaaattaatt
    28501 ttctacagga atacagaggc ggcgggaaga tatgccctta atgccactga aaaaccacat
    28561 aatttgtaca gcaataaaag tcacggccta atatggctaa caaacaatca attgtgatat
    28621 agttcacaaa attaatgaaa caaacagagt gtttcatttt tgtgttctgc aagccccgcg
    28681 atagccagag tgtaccacgc ctcccgtgaa caacgccgcg ctgtccaggg catcggctct
    28741 tttcagagga attgtttgat ggctaccctc accactggcg tggttcttct tcgctggcaa
    28801 cttcttagtg ccgtaatgat gtttctggcc agcacactca acatccgttt tcgtcggtct
    28861 gattatgtcg ggcttgcggt gatcagcagc ggtctgggcg ttgtttccgc ctgttggttc
    28921 gcaatggggt tgcttggcat cacaatggcg gacatcaccg ctatctggca caacatcgag
    28981 tcggtgatga tagaagagat gaatcagaca ccgccacaat ggccaatgat tttgacctga
    29041 tacgtagaa
//
LOCUS       DAJTQY010000049        28431 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.049, whole genome shotgun sequence.
ACCESSION   DAJTQY010000049 DAJTQY010000000
VERSION     DAJTQY010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28431
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.049"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            564..1334
                     /locus_tag="OO349_002196"
     CDS             564..1334
                     /locus_tag="OO349_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HCR5867254.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1325..1798
                     /locus_tag="OO349_002197"
     CDS             1325..1798
                     /locus_tag="OO349_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HCR5867255.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISSVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPRLWLYRKRPRV"
     gene            1905..2444
                     /gene="dnaT"
                     /locus_tag="OO349_002198"
     CDS             1905..2444
                     /gene="dnaT"
                     /locus_tag="OO349_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HCR5867256.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2447..3184
                     /gene="dnaC"
                     /locus_tag="OO349_002199"
     CDS             2447..3184
                     /gene="dnaC"
                     /locus_tag="OO349_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HCR5867257.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            3233..3727
                     /locus_tag="OO349_002200"
     CDS             3233..3727
                     /locus_tag="OO349_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HCR5867258.1"
                     /translation="MMKTIKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3981..6272
                     /gene="opgB"
                     /locus_tag="OO349_002201"
     CDS             3981..6272
                     /gene="opgB"
                     /locus_tag="OO349_002201"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HCR5867259.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQKTLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDSCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKSKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6410..7432)
                     /gene="lgoD"
                     /locus_tag="OO349_002202"
                     /pseudo
     CDS             complement(6410..7432)
                     /gene="lgoD"
                     /locus_tag="OO349_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
     gene            7571..8485
                     /locus_tag="OO349_002203"
     CDS             7571..8485
                     /locus_tag="OO349_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR5867260.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSQLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPRETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8700..10061
                     /locus_tag="OO349_002204"
     CDS             8700..10061
                     /locus_tag="OO349_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5867261.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLNFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IITGMFCSAAFTLIVPQATTSMMAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(10110..11774)
                     /gene="tsr"
                     /locus_tag="OO349_002205"
     CDS             complement(10110..11774)
                     /gene="tsr"
                     /locus_tag="OO349_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HCR5867262.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQPTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGITFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAVREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
     gene            complement(11893..12339)
                     /gene="hpaR"
                     /locus_tag="OO349_002206"
     CDS             complement(11893..12339)
                     /gene="hpaR"
                     /locus_tag="OO349_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HCR5867263.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEDIPGDNE"
     gene            12611..13900
                     /locus_tag="OO349_002207"
     CDS             12611..13900
                     /locus_tag="OO349_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HCR5867264.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPSDRVRVLAEGFPPLENPVVDEREVTTRKSFPTL
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPAPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEETAK"
     gene            13897..15363
                     /gene="hpaE"
                     /locus_tag="OO349_002208"
     CDS             13897..15363
                     /gene="hpaE"
                     /locus_tag="OO349_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HCR5867265.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWADLPMKERARLMRRLGDLIDQNVSEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALASPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVRALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            15365..16216
                     /gene="hpaD"
                     /locus_tag="OO349_002209"
     CDS             15365..16216
                     /gene="hpaD"
                     /locus_tag="OO349_002209"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HCR5867266.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     AEEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            16226..16606
                     /locus_tag="OO349_002210"
     CDS             16226..16606
                     /locus_tag="OO349_002210"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HCR5867267.1"
                     /translation="MPHFIVECTENIREEARLPELFASVNTALAATGIFPLGGIRSRA
                     HWIDTWQMADGKHDYAFVHMTLKIGSGRSLESRQEVGEMLFDLIKTHFASLMESRYLA
                     LSFEIAELHPTLNFKQNNVHALFK"
     gene            16674..17477
                     /gene="hpaH"
                     /locus_tag="OO349_002211"
     CDS             16674..17477
                     /gene="hpaH"
                     /locus_tag="OO349_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HCR5867268.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAESRTLKGHKIGLTSKAMQASSQISEPDYGALMDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV"
     gene            17488..18291
                     /gene="hpaI"
                     /locus_tag="OO349_002212"
     CDS             17488..18291
                     /gene="hpaI"
                     /locus_tag="OO349_002212"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HCR5867269.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGPGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     KAVRATRYPPASIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGNSSSISMAKQNNNSVY"
     gene            18313..19668
                     /gene="hpaX"
                     /locus_tag="OO349_002213"
     CDS             18313..19668
                     /gene="hpaX"
                     /locus_tag="OO349_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HCR5867270.1"
                     /translation="MTTSTLQYKDNKTVEIEHRVINKLFRRLIIFLFILFVFSFLDRI
                     NIGFAGLTMGKDLGLTSTMFGLAATLFYVTYVMCGIPSNMMLAKIGARRWIAGIMVVW
                     GIASTCTMFATSPETLYLLRMLVGIAEAGFLPGILVYLTWWFPAYHRARANALFMIAM
                     PVTMMLGLILSGYILALDGLWNLKGWQWLFLLEGLPSVILGVVTWFYLNDTPDQATWL
                     DENEKQALKTMILREQESAFAHASVPRSTLREVLTPAIMLYTLAYFCLTNTLSAINIW
                     TPQILQSFNTGSSNIMIGLLAAIPQFCTIFGMIWWSRRSDRLKERKKHTILPYLFAAA
                     GWLLASATDHSLIQLSSIIMASMGSFTAMTIFWTTPDQVISLQSRAVAMAVINAVGNV
                     GSAVSPLLIGILKDATGSFSSGLWFVAGLLVLGALVMTRIPMGSREHSLSSELIAPKP
                     Q"
     gene            19678..20568
                     /gene="hpaA"
                     /locus_tag="OO349_002214"
     CDS             19678..20568
                     /gene="hpaA"
                     /locus_tag="OO349_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HCR5867271.1"
                     /translation="MSSEAIFEHGYENIDLVREYDARYAGEDVHYETFARLANFFGRD
                     MRPHWHDRYFQLHYLVTGRITLQLDEHFYSVRAPLFVLTPPSVPHTFFTDPDSDGHVL
                     TVRQELIWPLVEKLWPGSGDAAMLQGICLSLEGMEHTQAALNHYWPIISLEFQQNKQG
                     REILLQSLAQSIFTILLREAPPNDISTCSVRGEMCLFQKFNRLIDEKYRQHLMVPEYA
                     TMLGMSESRLTELCRRFANQSPKRLIFERVSREARRLLLYSEQSINQIALDLGYKDPA
                     YFARFFNRMMGCPPTQFRGR"
     gene            20595..21532
                     /locus_tag="OO349_002215"
                     /pseudo
     CDS             20595..21532
                     /locus_tag="OO349_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601398.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
     gene            21759..23317
                     /gene="hpaB"
                     /locus_tag="OO349_002216"
                     /pseudo
     CDS             21759..23317
                     /gene="hpaB"
                     /locus_tag="OO349_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
     gene            23335..23847
                     /locus_tag="OO349_002217"
     CDS             23335..23847
                     /locus_tag="OO349_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HCR5867272.1"
                     /translation="MQLDEQRLRFHDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            24240..26390
                     /gene="btsT"
                     /locus_tag="OO349_002218"
     CDS             24240..26390
                     /gene="btsT"
                     /locus_tag="OO349_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HCR5867273.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            26440..26643
                     /locus_tag="OO349_002219"
     CDS             26440..26643
                     /locus_tag="OO349_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HCR5867274.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            26654..27610
                     /gene="yjiA"
                     /locus_tag="OO349_002220"
     CDS             26654..27610
                     /gene="yjiA"
                     /locus_tag="OO349_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HCR5867275.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQLYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
     gene            complement(27679..>28431)
                     /locus_tag="OO349_002221"
     CDS             complement(27679..>28431)
                     /locus_tag="OO349_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HCR5867276.1"
                     /translation="TKILENDFEHPSIRRKIASLSNLGVDEWIPPVMAFMNRMARTED
                     FNLDDFSQFITAFEKVYMHGWLKKQIKSQREMVCYSALVAINNDMPFDSVINQINQHA
                     DNSGFIAALDEDLYEPRPNQVNLIKAILLRLDMEQQDESVIKTYTGRITIEHILPQAL
                     VNEYWINRFQPQEHVYWLHKIGNLTLISGSKNSETQHYDFIKKKSIYEKLNSKSSFYL
                     TKDVCNSSEWGLAELKMRHEKMKTQLKKLWLV"
BASE COUNT         6959 a         7431 c         7127 g         6914 t
ORIGIN      
        1 cttaaatgtt tccagcaaat taatagatta agcgaataaa ttatccttag cattcacttt
       61 catatcagca tactcctgct gcacaacaac tccactgcat gtgtcagaag gttagcatca
      121 aatgataacc tggtgaaata gcctggagac atttaaaata tctacattaa gttgttagaa
      181 atacttaatg aaaaacagaa atctcattca tttaatttat aattaaatga tatctcttcc
      241 cgcagaatgc gggaatcaca caatcaatat ttattcaata aaatctgatg attttaactt
      301 gttaacacta aaaaacgcgc ccagctatct attatcctga cccgtaaagg ctaaacaata
      361 aattcattat agtgataata ttcatgtgat agtttgtttg cggcgagaga taattcgctt
      421 tttatcaccg agcttgtccc aataaccagg attaatcagc gcaattttga tcttaaccta
      481 ccgcaaaaca accgtgcttc atgatacaaa tggattctca ttatctgaac aatacgcagc
      541 acgtctatga caaagggcga gttatgcaaa ctgagcaaca gcgagccgta acgcggcttt
      601 gtatccagtg tggattattt cttttgcaac atggtgcgga aagcgcgttg gttgatgagc
      661 tttcctcacg actgggtcgg gcgctgggaa tggacagcgt cgaaagttct atctcttcga
      721 acgccatagt gctgacaact attaaagatg ggcaatgcct gacatcgaca cgtaaaaatc
      781 acgatcgcgg cattaatatg catgtggtga ctgaagtcca gcacattgtg attcttgcgg
      841 agcatcatct gctggattac aaaggcgtag agaaacgatt tagccaaatt caaccattac
      901 gttacccaag atggctggta gccttaatgg ttggcctttc ttgcgcctgt ttctgtaaac
      961 tcaataacgg tggctgggat ggtgccgtca tcaccttctt tgccagtacg accgcgatgt
     1021 atatccgcca gctgctggca caacgtcatc ttcatccaca gatcaacttt tgccttaccg
     1081 ctttcgccgc caccaccatt tccggattgc ttttgcaact ccccactttc agcaataccc
     1141 ccaccattgc gatggccgcc agcgttctgc tgctagtgcc gggctttccg ttgattaatg
     1201 ccgtcgccga tatgtttaaa ggccacatca ataccggact ggcacgctgg gcgatcgcca
     1261 gtctgctgac actggctacc tgcgtcggcg tagtgatggc actgacgatt tgggggctac
     1321 gcggatgggt gtgatcgaat ttctgttagc gttggcgcag gatatgatcc tcgccgccat
     1381 tcctgcggtc ggctttgcga tggtgttcaa cgttcccgtg cgggcgttac gctggtgtgc
     1441 gctgcttggc tcgataggtc atggttcccg aatgatcttg atgaccagcg ggttgaatat
     1501 tgagtggtca acctttatgg cttctatgct ggtcggtacc attggtattc aatggtcgcg
     1561 ctggtatctg gcgcatccga aagtgtttac cgtggcggcc gttatcccta tgttcccggg
     1621 catatcggct tataccgcaa tgatttcgtc ggtaaaaatc agccagttag gttacagcga
     1681 accgttgatg attaccctgt taaccaactt tcttacagct tcatcgattg ttggtgcgtt
     1741 atccatcggt ctttccattc ctagattatg gttgtaccgc aagcgccctc gcgtataaaa
     1801 ttgcctcgct taagcacacg gatgagagac agcctcctct cctccgtgtg ttactataaa
     1861 agttatctcc cttctcgttc atcgttccat atttgagaaa cagtatgtct tccagagttt
     1921 tgaccccgga cgtcgttggt attgacgccc tggtacacga tcaccaaacc gttctggcaa
     1981 aagctgaagg cggtgtggtt gccgtatttg ctaacaatgc cccggcgttt tatgccgtca
     2041 cgcctgcacg cctggctgaa ctgctggcgc tggaagaaaa gctagcgcgt ccgggaagcg
     2101 atgtcgctct ggacgaccaa ctctatcagg aaccgcaagc cgctcccgtt gctgtaccca
     2161 tggggaaatt cgccatgtat ccggactggc aacccgatgc cgattttatc cgcctggcgg
     2221 cgctatgggg cgtggcgcta agagagccgg tgaccaccga agaactggcc tcattcattg
     2281 cctactggca ggcggaaggt aaagtctttc accatgtgca gtggcaacaa aaactggcgc
     2341 gcagcctgca aatcggtcgt gccagcaacg gcggactgcc gaaacgagat gtgaatacgg
     2401 tcagtgaacc tgacagccaa attccaccag gattcagagg gtaacgatga aaaacgttgg
     2461 cgacctgatg caacgcctgc aaaaaatgat gcctgcccat atcaaacctg cattcaaaac
     2521 gggtgaagag ctactggcct ggcagaaaga acaaggggcg atccgctccg ccgctctcga
     2581 acgtgaaaat cgggcgatga aaatgcagcg cacctttaac cgctccggta ttcgtccact
     2641 gcatcagaac tgctcctttg agaactatcg cgttgagtgt gaagggcaga tgaatgcgtt
     2701 aagcaaagcg cgccagtatg tcgaagagtt tgacggcaac atcgccagct tcatcttttc
     2761 tggtaagcca ggaaccggca aaaaccatct ggcggcggca atctgcaacg agctgctgct
     2821 acgcggtaaa tccgtattga tcatcaccgt ggccgatatt atgtcggcga tgaaagatac
     2881 cttcaggaat agcggtacca gcgaagaaca actgcttaac gatctgagca acgttgatct
     2941 gctggtgatc gatgagatcg gcgtgcagac cgaatcgaaa tacgaaaaag tgatcatcaa
     3001 ccagatcgtc gatcgccgct cttcttccaa acgcccaacc gggatgctga ccaacagtaa
     3061 tatggaagag atgaccaagt tgctgggcga acgcgtgatg gaccgtatgc gcctgggtaa
     3121 cagtttatgg gtgatcttca actgggatag ctaccgtagc cgggtaacgg gtaaagagta
     3181 ttaatccccg tttctggcgg tatactgcga cacaattcca ccttgagtaa tgatgatgaa
     3241 aactattaaa catcttctgt gctgtgccat tgccgccagc gcattaattt ccaccggggt
     3301 gcatgctgcg tcctggaaag atgcgctctc cagcgccgcc agcgaacttg gcaaccaaaa
     3361 cagcacgaca caggaaggcg gttggtcact cgcgtcatta actaacttgc ttagcagcgg
     3421 aaaccaagcc ttaagcgcag ataacatgaa caatgccgca ggcattctgc aatactgcgc
     3481 gaagcaaaag ctggcttcgg taaccgatgc cgaaaacatc aagaaccagg tgctggaaaa
     3541 gctgggcctg aacagtgaag agcaaaaaga agacaccaac tatctggacg gtattcaggg
     3601 tttgctgaaa acaaaagatg gtcagcaact caatctggat aacatcggaa cgactccgct
     3661 ggcagaaaag gtgaaaacca aagcctgcga tctggtgtta aagcaggggc tgaacttcat
     3721 ttcctgaacc gcgaaacgcc gcgttttagc tgttacccgg ctgacgcggc tctctcctgc
     3781 ccttttccct gctcctggac ggttttcccc ctgttacacg ggaagcgacc gcgatcaaaa
     3841 aagtctgttt ataaactaat tcctaacaac atcgcatttt tttgacacta gaattgcagc
     3901 aatatggcga cgccattaca ttgtgcttct caaaaagctt aaacgttggc taaacggccc
     3961 ggtttagtga ggatcgccca ttgtcagaac tactctcttt cgcccttttt ctcgcctctg
     4021 tgctgattta cgcatggaaa gcgggacgta acacctggtg gtttgcagcc acgttaacgg
     4081 tgctggggct atttgtcgtt ttaaatatca ccctgtttgc cagcgactat tttactggcg
     4141 atggtattaa cgacgcggtt ctctatacct taaccaacag cctgaccggt gctggcgtca
     4201 gcaaatacat cctgccgggt atcggcattg tgctggggct gacggcggtg ttcggtgcgc
     4261 tgggctggat cctgcgccgt cgtcgtcatc atccgcacca ttttggttac agcctgctgg
     4321 cgctcttact ggcgctgggt tcggtggacg ccagcccggc atttcgtcag ataacggaac
     4381 tggtgaaatc ccagtcacgc gatggcgacc cggactttgc ggcttattat aaagagccgt
     4441 cgaaaactat ccctgacccg aaactcaatc tggtttatat ctacggcgaa agtctcgagc
     4501 ggacctattt tgataacgaa gctttcccgg atctcacgcc tgaactgggc gcgttgaaaa
     4561 atgaaggcct ggatttcagc cacacgcagc agctgccagg aacggattac acgattgcgg
     4621 gcatggtggc ttctcagtgc ggcataccgc tgttcgcccc ctttgaaggc aacgcctccg
     4681 cctctgtctc cagcttcttc ccgcagaaca tctgtctggg cgatatcctg aaaaactcgg
     4741 gttatcagaa ctatttcgtg cagggcgcaa atctgcgttt tgccggtaaa gatgtgttcc
     4801 tgaaatcgca cggcttcgac cacttatacg gctcagaaga gctgaaaagc gtggtagccg
     4861 acccgcacta tcgcaacgac tggggattct acgacgatac cgttctcgat gaagcgtgga
     4921 aaaagtttga agagctttcc cgctcaggtc agcgattctc actgtttacc ctgacagtcg
     4981 atacccatca cccggatggt tttatctcgc gcacctgtaa ccgcaaaaaa tatgattttg
     5041 acggtaagcc gaatcagtca ttcagcgcgg taagttgcag ccaggagaat atcgcgacgt
     5101 ttatcaacaa aatcaaagcg tcaccgtggt ttaaagatac tgtcatcgtc gtctcttctg
     5161 accatttagc gatgaacaac acggcgtgga aatacctcaa taagcaggat cgcaataacc
     5221 tgttttttgt cattcgtggc gacaagccgc agcaaaagac gctggcagtg aagcgtaaca
     5281 cgatggataa cggcgcgacg gtgctggaca ttctcggtgg cgataactat ctcggacttg
     5341 gtcgtagcag tttatccggg cagtcgatgt cggaaatctt cctcaatatc aaagagaaaa
     5401 cattggcgtg gaagccagat atcatccgcc tgtggaaatt ccctaaagag atgaaagagt
     5461 tcaccatcga ccagcagaaa aacatgattg ccttctcggg tagccatttc cgtttgccgc
     5521 tgctgttgcg ggtttcagac aaacgcgtgg aaccgctgcc ggaaagcgaa tactcagcac
     5581 cgctgcgttt ccagctggcc gatttcgctc cacgcgacaa tttcgtctgg gttgacagtt
     5641 gctacaagat ggcacaactc tgggctccgg aactggcact ttccaccgac tggtgtgtct
     5701 cgcaggggca gcttggcggt cagcaaattg ttcagcatgt tgacaaaaca acatggaaga
     5761 gcaagacggc atttaaagat acggtcatcg acatggcgcg ttacaaaggc aatgtcgata
     5821 cgctgaagat tgttgataac gatattcgct acaaagccga cagtttcatc tttaacgtcg
     5881 ccggtgcgcc agaagaggtg aaacagttta gcgggatttc ccgtccggag tcgtggggcc
     5941 gctggtccaa cgcgcagctg ggcgatgaag taaaaatcga gtacaagcat ccgctgccga
     6001 agaaatttga cctggtgatt accgccaaag catacggcaa taacgccagc cgtcctattc
     6061 cggtacgcgt aggcaatgaa gaacaaactc ttgtgctggg caatgaagtg accaccacca
     6121 cactgcattt cgataaccca accgatgccg acacactggt aattgtgccg ccggaacctg
     6181 tctcaaccaa cgaagggaat atcctcggac actcgccgcg taagctcggg atcggcatgg
     6241 tggaaattaa agtggtagaa cgtgaagggt aattcctgac tgatgtgtag gtcgcatagg
     6301 gtgcgaccta cacgccttct ccggcttaca acacattatt aagaaacaaa aaaaacagcg
     6361 cccaacactt ccccggacgc tgcatggcaa ctttaataat ctcgccttat cagaaagtaa
     6421 ttacaccctt aatcaactca cgattgttaa tcacatcgcg ctcgtaggtt tctgccagcg
     6481 tggcgaacgg atagcgatgg gttaacatca tgtcggcagt gattttccct tccgccatca
     6541 gtcgaccgac tttagcgaaa tcttccggcg tggcgttgcg gctgcccatc atcgtcgttt
     6601 cttttttatg gaattccgga tcggagaact gcaactcacc tttaaacaga ccgacaaata
     6661 ccaccgtgcc gccgtgacga atcagattca cggtattatt catcgcatgt tgattacctg
     6721 tcgcgtcgat cactttctgc gccagcgaac caccaaactg cgcccgcagc tgcgcgtcaa
     6781 aatcttcggc taacgggtcc agcacgggca actccagacg cgttgccaca tgttcacggc
     6841 gcgccggact ggtatccgcc accaccacct gtgcgccatc ggctttagcg attgccgccg
     6901 cgcccagacc gattggcccc gccccgacca ccagcacctg ctcgccggga gcaatggctg
     6961 cgcgacgcac cgcatgagcg ctaatagcga aaggttcaat caatgcggcc gcctgcgggt
     7021 caataccgtc tgcgggcaaa atgttcgcca ccggcacgct caaatactca ctaaaaccgc
     7081 catcctgatg cacgccaatg actgaaattt tttcacagca gttggtacgc ccgcttttac
     7141 acgccgggca ttgctgacag gcaacataag ggatcacagc aacttgctga ccatttttaa
     7201 gatcagcaat atttttaccc agcccaacaa tctccccaca tatttcatgg cctaaaaccc
     7261 gtggataact aaaaaatggt tgatttccac cctaggcatg aatatcggta ccgcaaatcc
     7321 cgacagactt aatttttatt aatgcttcat tgtcacccgg aataggtatc tcgcgttgtt
     7381 tccagactaa ttctttcggc tgctggcaaa ttaaaacatt catcgtagac atgattcggc
     7441 tatccatttt ttgttgcctt agatatcaat gaaaatgaaa aatggcagcg gaacggaaaa
     7501 tctgttttgt gaaaacacac gcataaaaat gttttaaatc gggttttaat ggactcacaa
     7561 ggaggaaaat atgagtcgtt cacaaaattt acgccacaat gtgattaacc aggtcattga
     7621 tgatatggcg cggggtcata tcccttcccc acttccatcg caaagtgcgc tggcggaaat
     7681 gtacaacatc agccgcacta cagtgcgtca tattctcagc caattacgcg aatgcggcgt
     7741 cttaacgcag gttggaaacg actatgttat tgcgcgtaaa cctgaccatg atgacggttt
     7801 cgcctgtact accgcttcaa tgagtgaaca aaacaaagtg tttgagcagg cttttttcac
     7861 gatgataaac cagcgtcagt tacgccctag ggaaactttt tctgaactgc aactggctcg
     7921 ggccgcgggt gtcagcccgg tggttgtaag agaatatctt ttaaaattcg gccgttataa
     7981 tcttattcaa agcgaaaagc gcggccagtg gagcatgaag caattcgatc aatcctacgc
     8041 cgagcagctg tttgagctac gcgaaatgct cgaaacacat tcacttcagc actttctcaa
     8101 cctgcctgat catgatcccc gctggctgca ggcgaaaacc atgctagaac gtcatcggct
     8161 actgcgtgac aacatcggca atagctttcg catgttctcg caacttgaca gagattttca
     8221 ctccttactg ctttcagctg ccgacaatat cttttttgat caatcgcttg agatcatctc
     8281 agtcatcttt cattttcact atcaatggga cgaaagcgat cttaaacagc gcaatatcat
     8341 tgccgtcgat gaacatatga ccattctcag cgctctgatt tgccgcagtg accttgatgc
     8401 cacccttgcg ctccgtaacc atttgaattc agctaaacaa tcaatgattc gatccattaa
     8461 tgagaacacg cgttatgctc attaagtacg gattaaaaac agcaaactat tagcgggtgc
     8521 acggttttat caaaagataa ataccgggaa attcccgctt acctatgctc acaatcagtc
     8581 gactaaaaat gtttttgtga gcggtagtaa agtcctaaaa ctttaacctg ccgttaatgt
     8641 caaccttgcg taatgcacaa ttacgtaaac aattaaaatt ataaatacag gagtccggcg
     8701 tggaaaaaga aaatatcacc ctcgatccgc gttcttcatt tactccatct tcgtcggcag
     8761 atattcccgt gccaccagat ggattagttc aacgcagtac ccgaattaaa cgcattcaaa
     8821 ccaccgccat gttgttatta ttttttgcgg cggtaatcaa ttatctcgac cgcagttcgc
     8881 tgtcggtagc aaatttaacg attcgtgaag aattgggatt aagtgccacc gaaatcggcg
     8941 ctttgctctc cgtgttttca ctcgcttacg gcattgcgca acttccttgc ggcccactat
     9001 tggatcgtaa aggcccacgc ctgatgctgg gactggggat gttcttctgg tcactgttcc
     9061 aggccatgtc tggcatggtg cacagcttta cgcagttcgt gttggtgcgt atcggtatgg
     9121 ggattggtga agcgccgatg aacccatgcg gtgtaaaagt cattaacgac tggttcaaca
     9181 tcaaagagcg cggacgcccg atgggcttct tcaacgcagc ttctaccatt ggcgttgctg
     9241 taagcccacc gattctggcg gcaatgatgc tggtgatggg ctggcgcggg atgtttatta
     9301 ccattggtgt actggggatt tttctcgcca tcggctggta tatgctctat cgcaaccgcg
     9361 agcacgtaga actgactgcc gttgaacaag cttatctcaa tgcaggtagc gtcaatgccc
     9421 gccgagatcc gctcaatttt gccgaatggc gcagcctgtt ccgtaaccgc acaatgtggg
     9481 gaatgatgct cggattcagt ggcatcaact acactgcgtg gctgtatctg gcctggcttc
     9541 ctggttacct gcaaacagcc tataacctgg atttaaaaag cacagggttg atggcggcta
     9601 tccctttcct gtttggggct gccgggatgc tggtcaacgg ttacgttact gactggctgg
     9661 tcaaaggggg aatggctccg attaaaagcc gtaagatctg cattattacc gggatgttct
     9721 gttctgccgc ctttacgctg atagtaccac aagcgacaac atccatgatg gcggttctgc
     9781 tgattggcat ggcactgttc tgtattcact ttgccggaac atcctgctgg ggcttgatcc
     9841 acgtcgcagt tgcttctcgc atgactgcgt cggtgggcag tatccagaac tttgccagct
     9901 tcatctgcgc ctctttcgcg ccgatcatta ctggttttat tgttgataca acccactcat
     9961 tccgtctggc actaatcatt tgcggttgcg tcaccgcagc gggagcactg gcgtacatct
    10021 tcctggttcg ccagccgatc aacgacccgc gtaaagatta aactccctat gcccgataac
    10081 attttgctta tcgggcattt ttatggcgat taaaacgttt cccagttctc cccgctatct
    10141 gccactgcca ttttacgcgg cgtagctggc gtcacggttt ttaccacagc cgatgtttca
    10201 cgctgctgtt gctgctgttg ctgaatccgg aacactgcca cagcttcggt caggcgactg
    10261 gcctgctctt ccagcgcggc ggcggcagcg gcagattctt ccaccagcgc ggcgttctgt
    10321 tgagttaccc ggtccatctc agcaaccgct aagccaacct gatcgatacc acggctctgc
    10381 tcatcagaag cagaagctat ttcgcccata atgtccgtca cgcgggtcac agcactgaca
    10441 atctccgcca ttgtttcccc ggcgctttcg accagcgtag agccaacatc cactttcccc
    10501 accgagtctt caatcaggct tttaatttca cgaaccgcct gggcgctacg ctgggccaga
    10561 ttacgcactt ctcccgcaac caccgcaaaa ccacgtcctt gctcacccgc acgcgctgct
    10621 tcaaccgccg cgttcaaagc cagaatattg gtctggaagg taatgccgtc aattacgctg
    10681 ataatatcgg cgattttctg cgaactggtg gagatatcgc gcatagtctg caccacgtta
    10741 tctaccactt tgccgccgcg ttgcgccgtt tcagaagcac ttaatgccag atggctggcc
    10801 tggcgcgcat tctcggcgtt ctgtttcacc gttgcggtca gttgctccat gctggctgcc
    10861 gtctcttcca gcgaagcggc ctgttgctcg gtgcgcgaag agagatcatt attgccggta
    10921 gcgatttcgc tggcaccgct atagatggca ttggccccgt tgcgcacatc accgacggta
    10981 cgcatcagct ctccctgcat atggcgcaaa ctctctgcca gttgccccat ctcattagag
    11041 ccatccacct caatcggttt caccagatcg ccgcctgcaa tatgacgaat gctgtcaatc
    11101 aggcgattca ttggcgctac cagcgaggct ttaataccga accagacggc gaagatgacc
    11161 gccagtacga cgatcatcac gcccaccaga atccacatcg cctggctgta ggaggcattg
    11221 ttatcgctga cggcgatatc atagagccga tcgttttgct ccatgtaagc cacatactgc
    11281 ttctcgaaac cgtcctgata tccctgggtc ggctgatcaa agaactcgtt gattttgcct
    11341 gcacctaaca gttggatcag ctccgccagc gcattgtgat aaatatcgta attacgtttg
    11401 atctctgccg ctgcggcggt gctctgacgc gggtcacgcg gcaacgcttc gtaatccgcc
    11461 cagttttttt ccgcctgttt cagcgaaata ctggcactct gcatcagctc agcaacggtt
    11521 gaaccgctac caatattatt ctgatccatc atgtagcgga tacccgcgcg gttgagggtg
    11581 ttacgcgtct gcaacaacgc gacccagctg ccattcagcg tgggttgctg ctggcgaatg
    11641 gtttgtaaaa cagtgaaatt ttctttgtca ttctttaagg cattaaaaaa cagaccgcct
    11701 gatgtcagtt gtaaaaggcc aaaaacggcc aaaaccagca gtaagctggt cacgatcttg
    11761 atacgtttta acatggtttc tctttcctgt ggaccgatgc aagattttcg gcctggaaag
    11821 caaaaacttt atgaaaaggt gtaacccgcc atccatggaa aaaccaggcg ctggttatca
    11881 gatactaaaa agttattcat tatcgcccgg gatgtcttct tgtcgggaat tgcccagagc
    11941 aataaattcc tctaacagat gggttaactg ttgcattttc tcggcagtaa actgagcttc
    12001 aatctgccga taggcttctt caatctgtgt ctgagcgcgg ttatacagcg cctgcccctc
    12061 tttggtcagc gagatataga gcttccgctg atcattgatc ggctttaatc gcaacactaa
    12121 accgtcgcgt tccatccgcg tgaggatccc ggtcaggctt gggcgcaaaa tgcaggcgcg
    12181 atacgccaga tcgtgaaaat ccattgatgg gctttccgcc agaatacgca cgatgcgcca
    12241 ctgttgctca gtcaaattat gccgtttaac gattgggcga aaataactca tcgccgcttc
    12301 tcgcgcctgg agcaacgcaa tggttagtga gtcgtgcatt atctttcccc tgaaaaattc
    12361 aaaatcatta atatagaaac aattaattat cacgttaccg ggactgagct gcccccgcaa
    12421 ttcttcattt tatcaaaatt gatgatattt tattaactta ctgatattta atattttatt
    12481 atctttaaat gtaaatagtt tgttaattag atcacattta tatcacttac ttttgccaaa
    12541 ctacgcaatc gggaataaaa gtaatcatta acatattaat gattaagatg atcgaatccg
    12601 aggagtggta atgaaaggca ctatcttcgc cgtagcgttg aaccatcgca gccaacttga
    12661 tgcctggcag gaagcgttcc agcaatctcc ctacaaagcc ccgcctaaaa ctgcggtctg
    12721 gtttattaaa ccgcgcaata cggtgattgg ttgcggtgaa ccgattccct ttccacaggg
    12781 tgaaaaagta ctgagcggtg cgactgttgc gctgattgtg gggaaaacgg cgacgaaagt
    12841 acgtgaagaa gatgcggcag agtacatcgc cggatatgcg ctggctaacg acgtcagcct
    12901 gccggaagag agcttttacc gtccggcaat caaagcaaaa tgtcgtgatg gattctgccc
    12961 cattggcgaa accgtggctc tcagcaatgt cgataatctg actatctata ccgagatcaa
    13021 cgggcgtcct gccgatcact ggaataccgc cgatttacaa cgtaacgccg cacagctgct
    13081 gagcgccctg agcgaatttg ccacgctgaa tccaggcgat gctattctgc tcggcacgcc
    13141 acaggcgcgc gtggaaatac agccaagcga tcgcgtgcgt gttctcgcag aaggtttccc
    13201 gccgctggaa aatccggtag tggacgaacg tgaagtgacc acgcgcaaga gcttcccaac
    13261 gctgccacac ccgcacggta cgctgtttgc cctcggcctg aactacgccg accacgccag
    13321 cgaactggaa tttaagccac cggaagagcc gctggtgttc ctgaaagcgc cgaataccct
    13381 cactggcgac aaccagacct ccgtgcgccc gaacaatatt gaatacatgc actacgaagc
    13441 ggagctggtg gtagttattg gcaagcaggc gcgtaacgtc agcgaagccg atgccatgga
    13501 ttatgtcgcg ggctacaccg tgtgtaacga ctacgccatt cgcgactatc tggaaaacta
    13561 ctaccgccct aacctgcggg tcaaaagccg cgacggactg acgccgatgc tttcaaccat
    13621 cgtgccgaaa gaggcgatcc cggccccgca taatctgacc cttcgcacct tcgtcaacgg
    13681 cgagttacgc cagcaaggca ccaccgccga tctgatcttc agcgtgccct tcctgatcgc
    13741 ctacttaagc gaatttatga ccctgaatcc gggcgacatg atcgccaccg gcacaccaaa
    13801 aggcttatct gacgtggtgc ctggcgatga agtagtggtg gaagtagaag gcgtgggccg
    13861 cctggtgaac cgaattgtga gtgaggaaac agcgaaatga aaaaagtaaa tcattggatc
    13921 aacggcaaaa atgttgcagg taacgactac ttccagacta ccaatccggc aacgggtgaa
    13981 gtgctggcgg atgtggcctc tggcggtgaa gcggagatca atcaggcggt agcggcagcg
    14041 aaagaggcgt tcccgaaatg ggctgatctg ccgatgaaag agcgtgcgcg cctgatgcgc
    14101 cgtctgggcg atctgatcga ccagaacgtg tctgagatcg ccgcgatgga aaccgcggac
    14161 acgggcctgc cgatccatca gaccaaaaat gtgttgatcc cacgcgcttc tcacaacttt
    14221 gaatttttcg cggaagtctg ccagcagatg aacggcaaga cctatccggt tgacgacaag
    14281 atgctcaact acacgctggt gcaaccggta ggcgtttgtg cgctggcgtc gccgtggaac
    14341 gtgccgttta tgaccgccac ctggaaggtc gcgccgtgtc tggcgctggg caataccgcg
    14401 gtgctgaaga tgtcggaact ctccccgctg accgccgacc gcctgggtga gctggcgctg
    14461 gaagctggta ttccggcggg cgtgctaaac gtggtacagg gctacggcgc aaccgcaggc
    14521 gatgcgctgg tccgtcatca tgacgtgcgt gccgtgtcgt tcaccggcgg taccgccacc
    14581 gggcgcaaca tcatgaaaaa cgccgggctg aaaaaatact ccatggagct gggcggtaaa
    14641 tcaccggtgc tgatttttga agatgccgat attgagcgtg cgctggacgc cgccctgttc
    14701 accatcttct caatcaacgg cgaacgctgc accgccggtt cgcgcatctt tattcagcag
    14761 agcatctatc cagaattcgt taaacgcttt gccgaacgcg ccaaccgtct gcgcgtgggc
    14821 gatccgaacg atccgaatac ccaggttagg gcgcttatca gtcagcaaca ctgggaaaaa
    14881 gtctccggct atatccgtct cggcattgaa gaaggcgcaa ccctgttggc gggcggcccg
    14941 gataaaccgt ccgacctgcc tgcgcacctg aaaggcggca acttcctgcg cccaacggtg
    15001 ctggcggacg tagataaccg tatgcgcgtt gcccaggaag agattttcgg gccggtcgcc
    15061 tgcctgctgc cgtttaaaga cgaagccgaa ggcttacgcc tggcaaacga cgtggagtac
    15121 ggcctcgcgt cgtacatctg gacacaggat gtcagcaaag tgttacgcct ggcgcgtggc
    15181 attgaagcag gcatggtgtt cgtcaacacc cagaacgtgc gcgacctgcg ccagccattt
    15241 ggcggcgtaa aagcctccgg caccgggcgt gaaggcggtg agtacagctt cgaagtgttc
    15301 gcggaaatga agaacgtctg catttccatg ggcgaccatc caattccgaa atggggagtc
    15361 tgatatgggt aagttagcgt tagccgccaa gatcacgcac gtaccgtcaa tgtatctctc
    15421 tgaactacca ggaaaaaacc acggttgtcg ccagggtgcg atcgacgggc ataaagagat
    15481 cagcaagcgt tgccgggaaa tgggcgtcga taccattatc gttttcgata ctcactggct
    15541 ggtcaacagt gcttatcaca tcaactgtgc agaccatttt gaaggcgtct acaccagcaa
    15601 cgagctgccg cattttattc gcgacatgac ctacaactac gagggcaacc cggagttggg
    15661 gcagctaatt gccgaggaag ccttaaagct cggcgtgcgg gcaaaagcgc acaatattcc
    15721 cagcctgaaa ctggaatacg gcacgctggt accgatgcgc tacatgaatg aagacaagca
    15781 cttcaaagtg gtctccattt cggctttctg cacggttcac gattttgccg acagccgcaa
    15841 gctgggcgaa gcgattctga aagcgatcga acagtacgac ggcaccgtgg cggtccttgc
    15901 cagcggttcg ttatcgcacc gctttattga cgatcagcgt gcggaagaag ggatgaacag
    15961 ctacacccgc gagttcgacc gccagatgga cgagcgtgtg gtgaagctgt ggcgcgaagg
    16021 ccagttcaaa gagttttgca atatgctgcc ggagtacgcc gactactgct acggcgaagg
    16081 caatatgcac gacacggtga tgctgctggg gatgctcggc tgggataaat acgacggcaa
    16141 ggtggagttt attaccgagc tgttcccaag ctctggcacc ggtcaggtta acgctgtttt
    16201 cccgctgccc gcgtaaggac gttttatgcc gcactttatc gtggaatgta ctgaaaatat
    16261 tcgtgaagaa gcccgcttac ctgagctgtt tgccagtgtt aacactgcac tggcggcaac
    16321 ggggattttt ccgcttgggg gaatacgtag ccgggcgcac tggatcgata cctggcaaat
    16381 ggctgatggc aagcacgatt acgcctttgt gcatatgacg ctaaaaatcg gcagcggacg
    16441 cagtctggaa agccgtcagg aagtgggcga aatgctgttt gatctgatta aaacgcactt
    16501 cgcctcactg atggagagcc gttacctagc gctttcattt gagattgcag agctgcaccc
    16561 gacgttgaat ttcaaacaga acaacgtaca cgcgttgttt aaataaattt gcaccagctt
    16621 ccaggccgga tgataaggcg ctgccgcatc cggcaatgcc acaggatatc gctatgttcg
    16681 ataaacacac ccacaccctg atcgcccagc gtctggatca ggcagaaaaa cagcgcgaac
    16741 agatccgcgc gatctcgctg gattacccgg agatcaccat cgaagacgcc tacgccgtgc
    16801 agcgcgaatg ggttcgcctg aaaatcgccg aaagtcgcac gctgaaaggc cacaaaatcg
    16861 gtctgacctc gaaagcgatg caggcaagct cgcagatcag cgaaccggat tacggtgcac
    16921 tgatggacga catgttcttc cacgatggca gcgatatccc gaccgatcgc tttatcgtgc
    16981 cgcgcattga agtggagctg gcttttgtgc tggcaaaacc gctgcgtggg ccaaactgca
    17041 cgctgttcga cgtttacaac gccacggact atgtgatccc ggcgctggag ctgatcgacg
    17101 ctcgctgcca caacatcgat ccggaaaccc agcgcccgcg caaagtattc gacaccattt
    17161 ccgataacgc cgccaacgcc ggagtgatcc tcggaggtcg tccgatcaaa ccagacgaac
    17221 tggatctgcg ttggatctcc gccctgatgt atcgcaatgg cgtgattgaa gaaaccggcg
    17281 tcgccgctgg tgtgctgaat catccggcaa acggcgtggc ctggctggcg aacaaactcg
    17341 caccgtatga cgtacaactg gaagccgggc aaatcattct cggtggttcg ttcacccgcc
    17401 cggttccggc gcgtaagggc gacaccttcc acgtcgatta cggcaacatg ggctccatta
    17461 gctgccgctt tgtttaagga gagaacgatg gaaaacagtt ttaaagcggc gctgaaagca
    17521 ggccgtccgc agattggatt atggctgggg ctgagcagca gctacagcgc ggagttactg
    17581 gccggaccag gattcgactg gttattgatc gacggtgagc acgcgccgaa taacgtgcaa
    17641 accgtgctca cccaactaca ggcgattgcg ccctacccca gccagccagt ggtacgccca
    17701 tcgtggaacg atccggtgca aatcaaacaa ctgctggacg taggcacaca aaccttactg
    17761 gtgccgatgg tacaaaacgc cgacgaagcc cgtaaagcgg tacgcgccac ccgttatccc
    17821 cccgccagta ttcgcggtgt gggcagtgcg ctggctcgcg cctcgcgctg gaatcgcatt
    17881 cctgattacc tgcaaaaagc caacgatcaa atgtgcgtgc tggtgcagat cgaaacgcgt
    17941 gaggcaatga agaacttacc gcagattctg gacgtggaag gggtcgacgg cgtgtttatc
    18001 ggcccggcgg atctgagcgc cgatatgggt tatgccggta atccgcagca cccggaagta
    18061 caggccgcca ttgagcaggc gatcgtgcag atccgcgaag cgggcaaagc gccggggatc
    18121 ctgatcgcca atgagcaact ggcaaaacgc tatctggaac tgggcgcgct gtttgtcgcc
    18181 gtcggcgttg acaccaccct gctcgcccgc gccgccgaag cgctggcagc acggtttggt
    18241 aatagttctt ctatttcgat ggcgaagcaa aacaacaact ctgtctacta aacgtacgat
    18301 gtggagcgca tcatgacgac ctctaccctg caatataaag ataataaaac tgttgaaatt
    18361 gaacatcgtg tcattaataa attatttcgt cgcctaatca tttttctttt tattctgttt
    18421 gttttttcgt ttcttgatcg catcaatata ggatttgccg gactgacaat gggcaaggat
    18481 cttggcctga cgtccacaat gtttggcctc gccgcaacac tcttttatgt cacgtacgtc
    18541 atgtgcggga tccccagtaa tatgatgtta gcgaaaattg gcgctcgtcg atggatcgct
    18601 gggatcatgg tggtctgggg aattgcttcc acctgcacaa tgtttgcaac aagccccgaa
    18661 acactctatc tactgcgtat gctggttggt attgctgagg cgggtttttt acctggtatt
    18721 ttggtttatc tgacctggtg gttcccggca tatcatcggg ccagagccaa tgccttattt
    18781 atgatagcga tgccagtcac tatgatgctg ggattgatcc tttcaggcta cattctggcc
    18841 ttggatggac tgtggaatct aaaaggctgg caatggctct ttttgctgga aggtctgcca
    18901 tcggtcattc ttggtgtcgt cacctggttt tatctcaacg acacaccaga ccaggcgacc
    18961 tggcttgatg aaaacgaaaa acaggcttta aaaacgatga ttctccgtga acaagagagt
    19021 gcatttgccc atgcatctgt tccacgctca accttacgtg aagtcctgac cccagccatc
    19081 atgctatata cacttgctta tttttgcctg actaatactc tgagtgcgat taatatctgg
    19141 acgccgcaaa tcctacagag tttcaatact ggcagtagca acatcatgat tggtttgctg
    19201 gcggccatcc cccaattctg cacaattttt ggcatgatct ggtggagtcg tcgttccgac
    19261 cgtcttaaag aacgcaaaaa gcatacgatt cttccctacc tgtttgccgc tgccggatgg
    19321 ttactcgctt ctgcgacaga tcacagcctc attcaactga gcagcatcat tatggcctca
    19381 atgggatcat ttaccgcgat gactatcttt tggacaacac cggatcaagt tataagcttg
    19441 caatcgcgtg ccgttgcgat ggctgtcatc aatgctgtcg gcaatgtagg ttcagcagtc
    19501 agtccattac ttatcggtat tttgaaagat gcaaccggca gttttagctc aggtttgtgg
    19561 tttgtagcag gattgctggt actgggcgcg ttagtcatga cccgaatccc tatggggagt
    19621 cgggaacact ctctttcatc tgaacttatc gcaccaaaac cgcaataagg agtcatgatg
    19681 tccagtgaag ctatctttga acatggttat gaaaatattg atttggtccg ggaatatgat
    19741 gcccgctacg ctggtgaaga tgtgcattat gaaacatttg cccggttagc gaatttcttt
    19801 ggacgcgata tgcgccctca ctggcacgat agatattttc aactccatta tctggtcact
    19861 ggacgcatta ctttacaact ggatgagcac ttctattctg tgcgcgcacc gctttttgta
    19921 ctaacaccac catcggtgcc acatactttc tttactgatc ctgatagcga tgggcatgtt
    19981 ctgaccgtac gtcaggagtt aatttggccc ctggtagaaa agctctggcc tggtagtgga
    20041 gatgccgcaa tgctacaagg tatctgtctg tcactggagg gaatggaaca tacacaggcg
    20101 gcattaaatc attattggcc gataatatcc ctggagtttc aacaaaataa gcaaggacgg
    20161 gaaattctat tgcaatcact ggcgcaaagt atttttacaa tcctactgcg tgaagcacca
    20221 ccaaatgata tttcaacatg cagcgtgcgc ggcgagatgt gtttattcca gaagtttaat
    20281 cggctgattg acgaaaaata tcgccaacat ttaatggtcc ctgaatatgc aacaatgctc
    20341 ggtatgagtg aatcacggct taccgagctg tgccgacgtt ttgccaatca atcgccgaag
    20401 agattgattt tcgaacgagt gagccgggaa gccagacggc ttctgctcta tagcgagcaa
    20461 agcatcaacc agatagccct tgatcttggc tacaaagatc ccgcctattt cgcccgtttt
    20521 ttcaaccgaa tgatgggttg cccaccaaca caattccgtg gtcgctgacc atttatcaac
    20581 ctgagagact cgacatgaaa atatctttac gctcactatt tgtggcgtgc ctgattgcgc
    20641 cagtggcatt cacctacgcc tccgaaccgc aataaatgaa aattgtacta cttggaacga
    20701 aaggtggccc ttctttatta aaagctgacc gtttaccgat ggcaacggca cttattattg
    20761 gcaataaaac ctagttaatt gatgctggtt acggggccag cttacaactg gtaaaaaata
    20821 atattccact gcgccagatt gataatattt tactcaccca tttacacagt gatcatattc
    20881 tggactatcc atcagtctta atgaatgcat gggctagcgg tctgcaggac aaaactattc
    20941 atgtattcgg tccgccaggc acccgagcca tgaccgctgc cagttggcag gtcttcgatc
    21001 gggacatcac actgcgtatg gaagaagaag gaaaacccga tcctcgcaaa ctcgttacag
    21061 ctcaagatgt tggtgaaggc gtagtttata aagatgatac cgtgaccgtg actgcattaa
    21121 aggtccctca ttctcctttc cctgacggtg aggcttttgc ttatcgcttc gacacacaag
    21181 gcaagcgaat tgtcttctca ggagatacct cgtactttcc cccactggca actttctcag
    21241 aaggcgctga tatacttgtg catgaggccg tacatgagcc ctccgtcgct aagctggcag
    21301 acagtattgg caatggtaaa acactggcaa aagccattgc ttctcaccat acgactattg
    21361 aggatgtagg caaaattgcc aatgaagcaa aagtgaaaaa actcatcctt agccacctgg
    21421 taccaatcac cgttccggat gaaacatgga agcaagaagc acagaaaact attcaggccc
    21481 tatcgtcgtt ggtcatgaaa atatgacgat taccattcca ttcgccccat aaaaaattcc
    21541 gtccccgtta gcccttgtta gcggggaata ttatgatttg ccttactgta agttgatcac
    21601 gatcgcagtt tactatttaa ctccaccttg ctgtcgaaaa gtaccaacgt tcgcctgaaa
    21661 cgtctcttcc cgacaaccgc gttcaacgct tcaattaaat aacaaaaaca acacacaaaa
    21721 ttaacaatcg tattccgatt aatactgtag aggtcgacat gaaaccagaa gatttccgcg
    21781 ccagtaccca acgtcctttc accggggaag agtatctgaa aagcctgcag gatggtcgcg
    21841 agatctatat ctatggcgag cgagtgaaag acgtcaccac tcatccggca tttcgtaatg
    21901 cggcagcgtc tgttgcccaa ctgtacgacg cgctgcacaa accggagatg caggactctc
    21961 tgtgttggaa caccgacacc ggcagcggcg gctataccca taaattcttc cgcgtggcga
    22021 aaagtgccga cgacctgcgc cagcaacgcg acgccatcgc tgaatggtca cgcctgagct
    22081 atggctggat gggccgtacc ccagactaca aagccgcttt cggttgcgca ctgggcgcga
    22141 atccgggctt ttacggtcag ttcgagcaga acgcccgtaa ctggtatacc cgtattcagg
    22201 aaactggcct ctactttaac cacgcgattg ttaacccacc gatcgtcatt tgccgaccga
    22261 taaagtgaaa gacgtttaca tcaagctgga aaaagagact gacgccggga ttatcgtcag
    22321 cggtgcgaaa gtggttgcca ccaactcggc gctgactcac tacaacatga ttggcttcgg
    22381 ctcggcacaa gtgatgggcg aaaacccgga cttcgcactg atgttcgttg cgccaatgga
    22441 tgccgatggc gtgaaattaa tctcccgcgc ctcttatgag atggtcgcgg gtgctaccgg
    22501 ctcgccatac gactacccgc tctccagccg cttcgatgag aacgatgcga ttctggtgat
    22561 ggataacgta ctgatcccat gggaaaacgt gctgatctac cgcgattttg atcgctgccg
    22621 tcgctggacg atggaaggcg gtttcgcccg tatgtatccg ctgcaagcct gtgtgcgcct
    22681 ggcagtgaaa ctcgacttca ttacggcact gctgaaaaaa tcactcgaat gtaccggcac
    22741 cctggagttc cgtggtgtgc aggccgatct cggtgaagtg gtggcgtggc gcaacacctt
    22801 ctgggcattg agtgactcga tgtgttcaga agcgacgccg tgggtcaacg gggcttattt
    22861 accggatcat gccgcactgc aaacctatcg cgtactggca ccaatggcct acgcgaagat
    22921 caaaaacatt atagaacgca acgttaccag tggcctgatc tacctccctt ccagtgcccg
    22981 tgacctgaac aatccgcaga tcgaccagta tctggcgaag tatgtgcgcg gttcgaacgg
    23041 tatggatcac gtccagcgca tcaagatcct caaactgatg tgggacgcca ttggcagcga
    23101 atttggtggt cgtcacgagc tgtatgaaat caactactcc ggtagccagg atgagattcg
    23161 cctgcaatgt ctgcgccagg cacaaagctc cggcaatatg gacaagatga tggcgatggt
    23221 tgatcgctgc ctgtcggaat acgaccagaa cggctggact gtgccgcacc tgcacaacaa
    23281 cgacgatatc aacatgctgg ataagctgct gaaataacgc agcaggaggt taagatgcaa
    23341 ttagatgaac aacgcctgcg ctttcatgac gcgatggcca gcctgtcggc agcggtaaat
    23401 attatcacca ccgagggcga cgccggacaa tgcgggatta cggcaacggc tgtctgctcg
    23461 gtcacggata caccaccgtc gctgatggtg tgcattaacg ccaacagtgc gatgaacccg
    23521 gtttttcagg gcaacggcaa gttgtgcgtc aacgtcctca accatgagca ggaactgatg
    23581 gcgcgccact tcgcgggcat gacaggcatg gcgatggaag agcgttttag cctctcatgc
    23641 tggcaaaaag gtccgctggc gcagccggtg ctaaaaggtt cgctggccag tcttgaagga
    23701 gagatccgcg atgtgcaggc gattggcaca catctggtgt atctggtgga gattaaaaac
    23761 atcatcctca gtgcagaagg tcacggactt atttacttta aacgccgttt ccatccggtg
    23821 atgctggaaa tggaagctgc gatttaagct ccagcccctt cacgcattcg tcgaaggggc
    23881 atttttattg tgatctcaat cacatatcta cccgatgaat aattcttgcc caaccgctca
    23941 ccgccattcc tgaccgctta catccctaaa ataaccactc agttatttac cttactttac
    24001 gcgcacgtat ctctggcaac atcactgcag gatagcggtc aatttacctc ctcaaacgca
    24061 acgcaaacct agaacggctt cggcctatga ttaacaaatt actctatgga ttttggtttg
    24121 caggaaggca gcaagtgagt gaatccccgg gagcttacaa aagtaagtgt cagaggtgaa
    24181 cgaacgcagc tgccgcacct gtaagccaaa agacgacgag taaaatgcca ggttttacta
    24241 tggatacgaa aaaactattc aagcacatac cctgggtgat tctcggaatc atcggtgcat
    24301 tctgcctcgc ggtagttgca ttacgtcggg gggagcacgt cagcgccctg tggatcgtgg
    24361 tcgcctctgt gtcggtgtat ctggtggcgt atcgctacta cagtctgtac atcgcccaga
    24421 aggtgatgaa actcgacccc acgcgcgcga cgcctgcggt tattaacaac gacggtctga
    24481 actacgttcc gaccaaccgt tacgtgttgt ttggtcacca cttcgccgct atcgccggtg
    24541 ctggtccgct ggtgggaccg gttctcgccg cgcagatggg ctacctgcct ggcacgctgt
    24601 ggctgctggc gggggtagtg ctggccggtg cggtacagga ctttatggtg ctgtttatct
    24661 cctctcgccg taacggcgca tctctcggtg agatgatcaa agaagagatg gggccagtac
    24721 cggggactat cgcgctgttt ggctgtttct taatcatgat catcatcctc gccgttctgg
    24781 cgctgattgt ggttaaagcc ctggccgaaa gtccgtgggg tgtcttcacc gtttgctcaa
    24841 ccgtaccgat tgcgctgttt atgggtatct acatgcgctt tatccgtccg gggcgtgtgg
    24901 gtgaagtctc tgttattggt atcgtgctgc tggttgcctc tatctacttc ggtggcgtga
    24961 ttgctcacga tccgtactgg ggtccggcac tgacctttaa agacaccacc attaccttcg
    25021 cgctgattgg ctatgcgttt gtttccgcac tgctgccggt gtggctgatc ctcgcaccgc
    25081 gcgactatct ggcaaccttc ctgaaaatcg gcgttatcgt cggcctggcg ctgggtatcg
    25141 tggtgctgaa cccggaactg aaaatgcctg cgatgaccca gtacattgac ggtactggcc
    25201 cgctgtggaa aggcgctctg ttcccgttcc tgttcatcac catcgcctgt ggtgcggtat
    25261 ctggcttcca cgcgctgatc tcttccggta cgacgccgaa actgctggct aacgaaaccg
    25321 acgcgcgttt cattggctac ggcgcaatgc tgatggagtc cttcgtggcg attatggcgc
    25381 tggttgcagc gtccatcatc gaaccgggtc tttacttcgc gatgaacacc ccgcctgctg
    25441 gccttggcat caccatgcct aacctgcatg aaatgggtgg cgagaacgcg ccgatcatca
    25501 tggcgcagct gaaagacgtt accgcacacg cggcagcgac cgtcagctcc tggggcttcg
    25561 tgatttcgcc agagcagatc ctgcaaaccg cgaaagacat tggtgagcct tctgtcctga
    25621 accgtgcagg tggtgcgccg acgctggcgg taggtatcgc tcacgtgttc cacaaagtgc
    25681 tgccgatggc tgacatgggc ttctggtatc acttcggtat tctgttcgaa gccctgttca
    25741 tcctgaccgc gctggatgcg ggtacccgtt ctggccgctt tatgctgcaa gacctgctgg
    25801 gtaacttcat cccgttcctg aaaaaaaccg actctctggt tgccggtatc atcggtactg
    25861 cgggctgtgt gggtctgtgg ggctacctgc tgtatcaggg cgtggtcgat ccgctgggcg
    25921 gcgttaagag cctgtggccg ctgttcggta tctccaacca gatgctggca gccgtagcgc
    25981 tggtactggg caccgttgtg ctgattaaga tgaagcgcac ccaatacatc tgggtaactg
    26041 ttgttccggc tgtatggctg cttatctgca ccacctgggc gctgggtctg aaactgttca
    26101 gcaccaaccc gcagatggaa ggcttcttct acatggcaag ccagtacaaa gagaagattg
    26161 ctaacggtac tgacctgacg gcgcagcaga ttgccaacat gaaccacatc gttgtgaaca
    26221 actacaccaa cgcaggcctg agtattctgt tcctgattgt ggtgtacagc atcatcttct
    26281 acggtttcaa aacctggctt gcggtgcgta acagcgacaa acgtactgac aaagaaacac
    26341 cgtacgttcc aatcccggaa ggcggcgtga agatctcttc gcaccactaa ccgtgtttag
    26401 ccccgcttcg gcggggcttt gttcttatca gagtgaacta tgtttggtaa cttaggacag
    26461 gcaaaaaaat atctcggcca ggcggcgaag atgttgattg gtattccaga ctacgacaac
    26521 tacgtcgagc atatgaagac caaccatccc gacaagccgt acatgagcta tgaagaattc
    26581 ttccgcgaac gccagaacgc gcgctacggc ggcgatggta aaggcggtat gcgctgttgt
    26641 taatggagag agcatgaacc cgattgcagt taccctactc accggttttt taggcgcggg
    26701 caaaactacc ctgctgcgcc atattcttaa cgaacaacac ggctacaaga ttgccgtgat
    26761 tgaaaacgaa ttcggcgaag tctccgttga tgatcaactg attggcgacc gcgccacgca
    26821 gatcaaaacg ctgaccaacg gctgcatctg ctgttcgcgc tccaacgagc tggaggacgc
    26881 actactcgac ctgctggata atctcgacaa gggcaatatt cagttcgatc gtctggtgat
    26941 tgaatgcacc ggcatggccg atcccggccc gattattcag acctttttct cccatgagat
    27001 tttatgccag ctctacctgc tggacggcgt gattgcgctg gtggatgcgg tacatgccga
    27061 tgagcagatg aaccagttca ccatcgccca gtcccaggtg ggctacgccg accgtattct
    27121 gctgactaaa accgacgtcg caggcgaagc agagaaactg cgtgaacgcc tggcgcgcat
    27181 caacgcccgc gcaccggtct acaccgtcac ccacggcgac atcgacctgg gcctgctgtt
    27241 caacaccaac ggttttatgc tggaagagaa cgtcgtcagc accaaaccgc gtttccactt
    27301 tatcgcggat aaacaaaacg atatttcgtc aattgtggtg gaactggatt acccggtaga
    27361 tatcagcgaa gtttcccgcg tgatggaaaa cctgctgctg gaatcggcgg ataaactgct
    27421 gcgttacaaa gggatgctgt ggattgacgg cgaacctaac cgcctgctgt tccagggcgt
    27481 ccagcgcctc tacagcgccg actgggacag gccgtggggc gatgaaaaac cgcatagcac
    27541 aatggtgttt atcgggattc agttgccgga ggaagagatt cgggcggcgt ttgcgggatt
    27601 gaggaagtaa gttcgggaaa gatcaacttt ttgagagttc caccgcaaca ttattacgag
    27661 tggaactctt tagatatgtc agaccaacca aagcttcttc aattgcgttt tcattttttc
    27721 atgtctcatt ttcagttcag ccaatcccca ttctgaagaa ttacaaacat cttttgttaa
    27781 ataaaaagag cttttactat taagtttctc ataaatagat ttctttttga taaaatcata
    27841 gtgttgtgtt tcagaatttt tggaaccact tatgagtgtt aaattaccta ttttatgcag
    27901 ccagtagaca tgctcttgag gttggaatct attaatccag tattcattaa ctaatgcttg
    27961 aggaagaata tgctcaattg tgattctccc cgtataggtt ttaatcacgc tctcatcttg
    28021 ttgctccata tctaagcgaa gcaaaattgc ttttattaag ttcacttgat tcggacgagg
    28081 ctcatataaa tcttcatcca gagcagctat aaaacctgaa ttatctgcat gttggtttat
    28141 ctgatttatc acagaatcaa aaggcatatc attatttatt gctaccagag cagaataaca
    28201 aaccatttct ctttggcttt taatttgttt ttttaaccac ccatgcatat ataccttctc
    28261 aaaggcggta ataaattgag aaaaatcatc taaattaaaa tcttctgtac gagccattcg
    28321 attcatgaat gccataaccg gaggaatcca ctcatctacc cctaaattac ttagcgatgc
    28381 tatttttcgt cgtattgaag ggtgttcaaa atcattctct aaaatcttag t
//
LOCUS       DAJTQY010000050        28404 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.050, whole genome shotgun sequence.
ACCESSION   DAJTQY010000050 DAJTQY010000000
VERSION     DAJTQY010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28404
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.050"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(<1..141)
                     /gene="yjdI"
                     /locus_tag="OO349_002222"
     CDS             complement(<1..141)
                     /gene="yjdI"
                     /locus_tag="OO349_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HCR5867277.1"
                     /translation="MDQALLDGGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKS"
     gene            322..1953
                     /gene="dcuS"
                     /locus_tag="OO349_002223"
     CDS             322..1953
                     /gene="dcuS"
                     /locus_tag="OO349_002223"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HCR5867278.1"
                     /translation="MRHSLPYHILRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILNALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            1950..2669
                     /gene="dcuR"
                     /locus_tag="OO349_002224"
     CDS             1950..2669
                     /gene="dcuR"
                     /locus_tag="OO349_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HCR5867279.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDAPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            3240..4580
                     /gene="dcuB"
                     /locus_tag="OO349_002225"
     CDS             3240..4580
                     /gene="dcuB"
                     /locus_tag="OO349_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HCR5867280.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            4658..6304
                     /gene="fumB"
                     /locus_tag="OO349_002226"
     CDS             4658..6304
                     /gene="fumB"
                     /locus_tag="OO349_002226"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCR5867281.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNLGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            6427..7056
                     /locus_tag="OO349_002227"
     CDS             6427..7056
                     /locus_tag="OO349_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HCR5867282.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLLEVTPVIIV
                     VPLLLATARRYPLTLLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVILLARFHCRQLRRYGLITG"
     gene            complement(7195..8616)
                     /gene="melB"
                     /locus_tag="OO349_002228"
     CDS             complement(7195..8616)
                     /gene="melB"
                     /locus_tag="OO349_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HCR5867283.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQSSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(8731..10074)
                     /gene="melA"
                     /locus_tag="OO349_002229"
     CDS             complement(8731..10074)
                     /gene="melA"
                     /locus_tag="OO349_002229"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HCR5867284.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALEDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICENMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPYIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKLGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPS"
     gene            10357..11265
                     /gene="melR"
                     /locus_tag="OO349_002230"
     CDS             10357..11265
                     /gene="melR"
                     /locus_tag="OO349_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HCR5867285.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSSWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            11464..13731
                     /gene="adiA"
                     /locus_tag="OO349_002231"
     CDS             11464..13731
                     /gene="adiA"
                     /locus_tag="OO349_002231"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HCR5867286.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSIHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKHHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            14056..14817
                     /gene="adiY"
                     /locus_tag="OO349_002232"
     CDS             14056..14817
                     /gene="adiY"
                     /locus_tag="OO349_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HCR5867287.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            14954..16291
                     /gene="adiC"
                     /locus_tag="OO349_002233"
     CDS             14954..16291
                     /gene="adiC"
                     /locus_tag="OO349_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HCR5867288.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            16395..18038
                     /gene="eptA"
                     /locus_tag="OO349_002234"
     CDS             16395..18038
                     /gene="eptA"
                     /locus_tag="OO349_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HCR5867289.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILIGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWLKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRATNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPDQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSK"
     gene            18035..18703
                     /gene="basR"
                     /locus_tag="OO349_002235"
     CDS             18035..18703
                     /gene="basR"
                     /locus_tag="OO349_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HCR5867290.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEDGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            18704..19804
                     /gene="pmrB"
                     /locus_tag="OO349_002236"
     CDS             18704..19804
                     /gene="pmrB"
                     /locus_tag="OO349_002236"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HCR5867291.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(19780..19869)
                     /gene="pmrR"
                     /locus_tag="OO349_002237"
     CDS             complement(19780..19869)
                     /gene="pmrR"
                     /locus_tag="OO349_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HCR5867292.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(19981..21483)
                     /gene="proP"
                     /locus_tag="OO349_002238"
     CDS             complement(19981..21483)
                     /gene="proP"
                     /locus_tag="OO349_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HCR5867293.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFMGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(21747..22625)
                     /locus_tag="OO349_002239"
     CDS             complement(21747..22625)
                     /locus_tag="OO349_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HCR5867294.1"
                     /translation="MTKTLLDGPGRVLESVHPRFLVDLAQGDDARHPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVVLLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLVGAMVEEIFSVNLA"
     gene            complement(22622..24850)
                     /gene="crfC"
                     /locus_tag="OO349_002240"
     CDS             complement(22622..24850)
                     /gene="crfC"
                     /locus_tag="OO349_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HCR5867295.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELCKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARYELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDAVNAQLAALFQD
                     DSEPLSEIRTRCETLLQTAQNTISRDFTLRFAEMESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHSWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            25252..25587
                     /locus_tag="OO349_002241"
     CDS             25252..25587
                     /locus_tag="OO349_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HCR5867296.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            25745..26188
                     /gene="yjdN"
                     /locus_tag="OO349_002242"
     CDS             25745..26188
                     /gene="yjdN"
                     /locus_tag="OO349_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HCR5867297.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            26321..27109
                     /gene="phnC"
                     /locus_tag="OO349_002243"
     CDS             26321..27109
                     /gene="phnC"
                     /locus_tag="OO349_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HCR5867298.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHSEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQLFDNERFDHLYRSINRIEENAKAA"
     gene            27134..28150
                     /gene="phnD"
                     /locus_tag="OO349_002244"
     CDS             27134..28150
                     /gene="phnD"
                     /locus_tag="OO349_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5867299.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLTPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLILGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEIQSVKGNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            28256..>28404
                     /locus_tag="OO349_002245"
     CDS             28256..>28404
                     /locus_tag="OO349_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane channel protein of phosphonate
                     transporter"
                     /protein_id="HCR5867300.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLDVLVVSWQGAEMAPLTLIKDG
                     GNMAT"
BASE COUNT         7295 a         7422 c         6935 g         6752 t
ORIGIN      
        1 cgactttcgt ttgagattaa ataacttgcc gttgccacgt acgcaattgc cagaatgctg
       61 acatatcgca gtgttgaaat agacatcgat cttttcgccg gtataacagc gataaccccc
      121 gtccagtagc gcctgatcca tgaccttccc tctctcatca gtatgattat cgctataagc
      181 atagccccgg aatcttcata cagcactggc agtttccgtt gccagagtct cttcccctat
      241 atagccaatc atttttactc cctttgaatt acccgcctca tcagagataa tgcttaagaa
      301 atttgtcaca caaggaagct gatgagacat tcattgccct accacatatt acgcaaacgt
      361 ccgatgaaat tgagtaccac tgtgatctta atggtcagcg cggtactgtt ctcggtgcta
      421 ttggtggtgc atctgattta cttctcgcaa atcagtgata tgacgcgaga cgggctggcc
      481 aacaaggcac tggcagtggc gcgtaccctc gccgactcgc cggaaatccg tcagggcttg
      541 cagaaaaaac cgcaggagag tggcatccag gccatcgcgg aagccgtgcg caaacgcaac
      601 gatctgctgt ttattgtcgt taccgatatg caaagtcttc gctactcaca tcctgaagcc
      661 cagcgtattg gtcagccatt taaaggtgat gacatcctta atgcgctgaa tggcgaagaa
      721 aatgtcgcta tcaatcgcgg ttttctggcg caggctttac gcgtatttac ccccatctac
      781 gatgaaaatc ataaacaaat tggcgtggtg gcgatcggcc ttgagttaag ccgcgtgacc
      841 caacagatca atgacagtcg ctggagcatt atctggtcgg tattatttgg catgctggtc
      901 ggactgattg gcacctgcat tctggttaag gtactgaaaa aaatcctttt cggcctggaa
      961 ccctacgaaa tctccacgct gtttgagcaa cgccaggcca tgttgcagtc tatcaaagaa
     1021 ggcgtcgttg ccgtggacga tcgcggcgag gtcacgctga tcaacgatgc cgcacaagaa
     1081 ttgctgaatt accgtaagtc gcaggacgat gagaaactgt cgacgctcag ccactcatgg
     1141 tcacaggtgg tagatgtctc ggaagtgtta cgcgacggta cgccgcgccg cgacgaagag
     1201 attacgatta aagaccggct attactgatc aacaccgttc cggtgcgcag taatggcgtt
     1261 atcatcggtg ccatttcaac cttcagggac aaaactgaag ttcgtaaact gatgcagcga
     1321 ctcgacggtc tggtcaacta tgctgacgca cttcgtgaac gatcccacga atttatgaat
     1381 aaattgcatg tgattctcgg attattgcat ctgaagagtt ataagcagtt ggaagattac
     1441 attctcaaaa cagccaataa ctatcaggaa gagattggct ctctgctggg taagatcaaa
     1501 tctccggtta tcgctggttt tttaatcagc aagattaacc gcgcgaccga tttaggccat
     1561 acgctgattt taaacagtga aagccagctg ccagacagcg gcagtgagga ccaggtcgcg
     1621 acgctgatta ccacattggg aaatctgata gaaaacgcgc tggaagcatt agggccggaa
     1681 cccggtggcg aaattagcgt aacattgcac taccgtcacg gctggctgca ctgcgaagtt
     1741 aacgatgatg gaccggggat cgcacccgac aaaatcgatc acatttttga caaaggtgtc
     1801 tcgacaaaag gaagcgagcg aggcgtcggt ttagcacttg tcaaacaaca ggtagaaaat
     1861 ctcggcggca gcatcgccgt ggaatcggaa ccggggattt tcacacaatt ttttgtccag
     1921 ataccctggg acggggagag gtcgaacaga tgatcaatgt attaattatc gatgacgacg
     1981 caatggtcgc ggagctgaat cgccgatacg tagcacaaat cccaggcttt caatgctgtg
     2041 gaacagcctc gacgctggag aaagccaaag agattatctt caatagcgat gcgcctatcg
     2101 acctgatatt gctcgatatc tatatgcaaa aagagaacgg gctcgattta ctgcctgtcc
     2161 tgcataacgc gcgttgcaaa agtgatgtga ttgtcatctc ctccgcagcc gatgcggcaa
     2221 ccattaaaga ttcgctgcat tacggtgtcg tggattacct gatcaaaccc ttccaggctt
     2281 cccgctttga agaggcgctc accggctggc ggcaaaagaa aatggcgctg gaaaaacatc
     2341 agtattatga ccaggccgag cttgatcagc taatccacgg cagcagctcc aacgaacagg
     2401 atcctcgtcg cttgccaaaa ggcttaacgc cgcagacgtt acgcacgctg tgccagtgga
     2461 ttgacgcgca tcaggactat gagttttcaa ccgacgagct ggctaacgag gttaacatct
     2521 cgcgtgtttc ctgccgaaaa tacctcatct ggctggtcaa ctgccacatc ttgttcacca
     2581 gtatccatta tggcgtcacg gggcgtccgg tttaccgtta ccgtattcag gcagaacact
     2641 actcactgct gaaacaatat tgccaataac ggatcgctgg ttatctgtaa gtaataatta
     2701 ttaccgtaat gcaaaatacc cctggcacaa gttacatcgt taatcagtaa aacttgtgcc
     2761 agatcaaata taattatccc tccatcatcg ttaaaaatta atatctcttc agatgaacgg
     2821 tgtttttaat ttcaaaacga taacaaaagt taattaacta ttatgtcacc cgcattatgt
     2881 gtatttttac ccacaaatgg gtagatcaga ttaatctata aacctaatga tatctgccct
     2941 gagaacaaaa aatagaccga taaatatcaa taagataaca gcaaacaaaa cattaacatc
     3001 tgcgcagtac aaactataaa cccatcgcca gagagttttt ctctctgaaa aagccgctta
     3061 tcacagtgca taaatttgcc gctgctttaa tcagccaata ttcactgtga ggtatttgct
     3121 aaagccggta acgaccaaac ggatatttag tcaggctctg aaaacagttc atacaaaaca
     3181 gaacgtgact gtgatctatt cagcaaaaat ttaaatagga ttatcgcgag ggttcacaca
     3241 tgttatttac tatccaactt atcataatac taatatgtct gttttatggt gccagaaagg
     3301 gtggtatcgc gctgggttta ttaggcggta tcggtctggt cattctggtc ttcgtcttcc
     3361 accttcagcc aggtaaacca ccagttgatg tcatgctggt tatcattgcg gtggtggcgg
     3421 catcggcgac cttgcaagct tcgggcggtc ttgatgtcat gctgcaaatt gccgagaagc
     3481 tgctgcgccg caacccgaaa tatgtctcaa ttgtcgcgcc gtttgtgacc tgtacactga
     3541 ccattctttg cggtacgggt catgtggttt acaccattct gccgatcatc tacgacgtcg
     3601 ccattaagaa caacatccgt ccggaacgtc cgatggcggc aagttctatc ggtgcacaga
     3661 tggggattat cgccagtccg gtgtcggttg cggtcgtgtc tctggttgcg atgctgggta
     3721 atgtcacctt tgatggtcgc catcttgagt tcctcgatct gctggcaatc accattccat
     3781 cgacgttaat cggtatcctg gcgatcggta tcttcagctg gttccgcggt aaagatctgg
     3841 ataaagacga agagttccag aaattcatct ccgtaccgga aaaccgtgag tatgtttacg
     3901 gtgataccgc gacgctgctg gataaaaaac tgccgaaaag caactggctg gcaatgtgga
     3961 ttttcctcgg ggcaatcgct gtagtcgccc ttcttggtgc tgattcggac ctgcgtccat
     4021 ccttcggcgg caaaccgctg tcgatggtac tggttattca gatgtttatg ctgctgaccg
     4081 gggcgctgat tattatcctg accaaaacca atcccgcgtc tatctcaaaa aacgaagtct
     4141 tccgttccgg tatgatcgcc atcgtggcgg tgtacggtat cgcatggatg gcagaaacca
     4201 tgttcggtgc gcatatgtct gaaattcagg gcgtactggg tgaaatggtg aaagagtatc
     4261 cgtgggcgta tgccattgtt ctgctgctgg tttccaagtt tgtaaactct caggctgcgg
     4321 cgctggcggc gattgttccg gtcgcgctgg cgatcggcgt tgatccggca tacatcgtgg
     4381 cttcagcacc ggcttgctac ggttattaca tcctgccgac ttatccgagc gatctggcag
     4441 cgattcagtt tgaccgttcc ggcaccaccc acatcggtcg cttcgtcatc aaccacagtt
     4501 ttattctgcc ggggttgatt ggtgtgagcg tatcgtgcgt cttcggctgg atcttcgccg
     4561 cgatgtacgg gttcttataa atgcactttg cgtgccgccc gtgactacgc ggcacgccat
     4621 tttcgaataa caaatacaga gttacaggct ggaagctatg tcaaacaaac cctttatcta
     4681 ccaggcacct ttcccgatgg ggaaagacaa taccgaatac tatctactca cttccgatta
     4741 cgttagcgtt gccgacttcg acggcgaaac catcctgaaa gtggaaccag aagcactgac
     4801 cctgctggcg cagcaagcct ttcacgacgc ttctttcatg ctacgtccgg cacaccagaa
     4861 acaggttgcg gctattcttc acgatccaga agccagcgaa aacgacaagt acgtggcact
     4921 gcaattctta agaaactccg aaatcgccgc caaaggcgtg ctgccgacct gccaggatac
     4981 cggcaccgcg atcatcgtcg gtaaaaaagg ccagcgcgtg tggaccggtg gcggtgatga
     5041 agaagcgctg tcgaaaggcg tctataacac ctatatcgaa gataacctgc gctactcgca
     5101 aaacgcggcg ctggacatgt acaaagaggt caacaccggc actaacctgc ctgcgcaaat
     5161 cgacctgtac gcggtagatg gcgatgagta caaattcctt tgcgttgcga aaggcggcgg
     5221 ctctgccaac aaaacgtatc tctaccagga aaccaaagcc ctgctgactc ccggcaaact
     5281 gaaaaacttc ctcgtcgaga aaatgcgtac cctcggtact gcagcctgcc cgccgtacca
     5341 tatcgcgttt gtgattggcg gtacgtctgc ggaaaccaac ctgaaaaccg tcaaattagc
     5401 aagcgctcac tattacgatg aactgccgac ggaagggaac gaacatggtc aggcgttccg
     5461 cgatgtccag ctggaacagg aactgctgga agaggcccag aaactcggtc ttggcgcgca
     5521 gtttggcggt aaatacttcg cgcacgacat tcgcgttatc cgtctgccac gtcacggcgc
     5581 atcctgcccg gtaggcatgg gcgtctcctg ctccgctgac cgtaacatta aagcgaaaat
     5641 caaccgcgaa ggtatctgga tcgaaaaact ggaacacaat ctaggtcagt acattccaca
     5701 agaactgcgt caggccggtg aaggcgaagc ggtgaaggtc gaccttaacc gcccgatgaa
     5761 agagatcctc gcccagcttt cgcaatatcc ggtttccact cgtttgtcgc tcactggcac
     5821 cattattgtg ggtcgagata ttgcacacgc caagctgaaa gagctgattg acgccggtaa
     5881 agaacttccg cagtacatca aagatcaccc gatctactac gcgggtccgg cgaaaacccc
     5941 tgccggttat ccatcaggtt cacttggccc aaccaccgca ggtcgtatgg actcctatgt
     6001 ggatctgctg caatcccacg gcggcagcat gatcatgctg gcgaaaggca accgcagcca
     6061 gcaggttacc gacgcgtgtc ataaacacgg cggcttctac ctcggtagca tcggcggtcc
     6121 ggcggcggta ctggcgcagc agagcatcaa gcatctggaa tgcgtcgctt atccggagct
     6181 gggtatggaa gctatctgga aaatcgaagt agaagatttc ccggcgttta tcctggtcga
     6241 tgacaaaggt aacgacttct tccagcaaat cgtcaacaaa cagtgcgcga actgcactaa
     6301 gtaacctctt cggcccagcg cctgacagca tgctgccagg tgatccccct gggccacctc
     6361 ttttgcgatt gtaatttcat gcttgctggt gaatagtcag tattttccct gacttgcgaa
     6421 ctcaccatga cccgcacact caagccgtta attcttaaca ccggcgcact ggcgctaacg
     6481 ttaatcctga tttataccgg catttcggcc catgacaaac tcacctggct gctggaagtg
     6541 acaccggtga ttatcgtcgt gccgctacta cttgccaccg ccagacgtta cccgttaacg
     6601 ctgctgctct atacgctcat cttctttcac gccatcatcc tgatggtagg cgggcagtac
     6661 acctacgcga aagtccccgt tggttttgag gtgcaggaat ggctggggtt gagtcgtaat
     6721 ccgtatgaca agctggggca ctttttccag gggctggtgc ctgcactggt ggcacgagaa
     6781 attctcgttc gcgggatgta cgtgcgcgga cgtaaaatgg tggcgtttct ggtctgttgc
     6841 gtggcgctgg cgataagcgc gatgtatgaa ttaatcgagt ggtgggcggc actggcgatg
     6901 ggccagggag cggatgattt tctcggtacc cagggcgacc agtgggacac gcaatccgat
     6961 atgttctgcg cgctgcttgg cgcattaacg acagtgatat tgctcgctcg ctttcattgc
     7021 cgccagctac ggcgctatgg cttgataact ggataacaac gtgcactttg ttgatgccgg
     7081 atgcggcgtg aattccttat ccggtctaca aaatttgcaa atccaaagca ttacattaag
     7141 acgtggcctg ataaacatat cgcatcaggc cattttcgtt agctattgtc cacgtcaggc
     7201 tttcacatca ctcactgcac cgaccggaat atcagcatga acaggctcgg gcggtacttt
     7261 gcgatattta tccagcagat gaatctggat cctgcgcagc gtgtcgccat tgaggcgata
     7321 gaaacggaag tagagaatca gcgttaccat gaaaaacaga gtcggtagag caatcataat
     7381 aaactgcata cctaataggg cttgcgtaga ctgttcaaca ttcggtacat agccaatcat
     7441 ccctaacaca accgcaatga aaaaagccgc aaaggctgaa ccgcccttca ccaccatagt
     7501 ctgcacggag taagcgatgc tttcacagcg tacgtgcagt ttatattcac cgtaatcaac
     7561 ggtatctgcc accatgatga cctgtaatac ccagaaaagc gccgttccca cattcagcaa
     7621 aatacccgca atcacaatga ggacgacgtt gtgatagctc attaatgcca ttaacaggag
     7681 aacaccacag cttaacaccg gaagaataga tgctccggcc cataaaatgc gtcgggataa
     7741 tgatttaact aagcgtggga agaatactaa cgtcaccagg ttagcagctc ccgcatacga
     7801 cagataatag gggaacaaat ccgcatcacc gataacatat gagaaataat agatagcaaa
     7861 tccggtaata atgttgctgg ctacattata agcaagagcc atacccaaga ggcatgaaag
     7921 ctgatcgttt ttataaataa gcgcaacgat ggctttaagt gtcagatggc ttccttcagc
     7981 agacgattga ttgtctgacg aaaagacttc atgcacattg cgcagagtga tgatggttga
     8041 aacaataaaa aaggcgatca ataccagagt gaacatctga aagccaaatc cccgatcgcc
     8101 accgccgaca taattaacaa atggtagcgt cacacctgcc gtaacaaagc ctgccagact
     8161 ggcaaaaaaa cgcggataag gaaccagttg ttcgcgttca cgtttatcga gcgtgatggt
     8221 tggaaccagc gaccagaagg gaatatccat aatggtgtaa gtcatgcccc agaggatgta
     8281 ggtcacgcaa acaaagacaa tctgagtagt accttcaaac agatgcgcac taaagaggag
     8341 aaataagatt acagagtttg ccaacgtacc gatcaggatc cagggtttga acttacccca
     8401 tcgcgatcgc gtagcattta caatccatcc cataatcgga tcgttaatag catcccagat
     8461 cctcgccacc agaaacaaag taccgaccaa acccacagac agcccgacga catcggtgta
     8521 gtaatacatg aggtacatat acacaatgcc gatcgcaaaa tccttcccga acgctccaaa
     8581 tccataactg agttttgtag tcattgaaat gctcataggg tatcgggtcg ctgttaccaa
     8641 cgcccgcctt ctgttattgg tatcgtcaga ggtgtgcgcc ggaataaatc ccccggcgca
     8701 gtagcgttta gtcgcgtttt aacggtgcaa ctagcttggc agccagtcgc cgtgggcggc
     8761 aatcaggtcg tcaacaagag catatatttc gtcaatgccc agcacggcgg cagtatgcgg
     8821 gtccatcatc gcggcgtggt aaacacggtc gcgattttcc gtaagaatag cttcggtcag
     8881 cagcgtctgt acgttgatgt tggtttgcat cagggcggcc agatgcgaag gtagcgtacc
     8941 gactttggtc ggctgaatgc cattagcatc aaccagacag gctacttcca cgcaacatcc
     9001 ttgtggcagg ttatcaatca aaccatcgtt acggacgttg ccgtaaatca cactcggctc
     9061 gccagtccag atagcgttca tgattgtgct ggcatattcc cgtgacggtt taatatcaat
     9121 ccgggaggcg tttttatact cctccagctc tttatgccag ttcgccagct gctcgacgca
     9181 gcgtttcggg tactcatcca gcggtacttt ataacgctca atcaaatcct cacgacctag
     9241 cttaataaac cacggcgtat attcggcaaa atgttctgac gattccgtga cgaagtagcc
     9301 cagctttttg aacatttcgt agcgcacaat attctggcag cgagtattac catgaatatt
     9361 tggcttcggt gcctgccctg cttcataagc cgccagcagt tccgggtaga gattcacgta
     9421 actgccgtcg gcggttttgc gctccagctc caggtaaaac gccatatggt tgatacctgc
     9481 gcaacggtaa cgcagcgtag ccgggtcgat attgaggtca cgcgccagct cttccgccgt
     9541 tccctgcacc gaatggcaca gcccgacctg tttgatatac ggatagcggg catacatcgc
     9601 ccaggtattc atcgccattg ggttaacata gttgagcatg gtggcatcgg ggcagacttc
     9661 cgtcatgttc tcgcaaattt gccacagatg cggaatggta cgtagcgcgc gcataatacc
     9721 gcccggcccc aacgtatcgg caatggtttg ttccagacca tgccgcttac agacctcgaa
     9781 atcagtcacc gtgcaaggtt cataaccgcc aatctgaaat gccaccacga caaaatcggc
     9841 atcctctaag gcttctttct gttgggtgtg gcaggtgatt ttgccgctgg cccctgctga
     9901 atccatcagc ttacgcacca caatatgcga ctcttccagg cgggtggggt caatgtccat
     9961 cagggcaata tgcgccgttt tcagcgcctc gcgatggaac acatcaccaa gaatattttt
    10021 aacgaaaatc gtcgaaccag cgccgataaa tgtaattttg ggtgcagaca tcatgcagat
    10081 ctcctggctt gcttgaataa cttcatcatg gcaggcgaat ctgcagaaaa cctccggctt
    10141 ccaggttgag cattcccata aactcagatc ctgcgtgaag cagcagtaaa tctgagttta
    10201 tgggaataaa ttacctgaaa agtagagttt tcggcctcat gatgctcgga cagtcccctc
    10261 gcccctctgg gaagagggtg aaaaccgtgc tcccactcgc agtcatcctc cctcactcct
    10321 gccataattc tgatattcta ggaaagagag ccatccatga atacagatac gtttatgtgc
    10381 agcagcgacg aaaaacagac ccgcagcccg ctgtcgctgt attcggaata ccagcggatg
    10441 gagattgagt ttcgcgcacc gcatatcatg cccaccagcc actggcatgg tcaggtcgaa
    10501 gtgaatgtgc ctttcgatgg cgatgtggaa tacctgatca acaatgaaaa agtgaatatc
    10561 aatcagggtc atatcacgct gttctgggcc tgtacaccgc accaactaac agataccgga
    10621 acctgtcaga gcatggcgat ttttaatctg ccgatgcatc tgtttctctc ctggccgctg
    10681 gataaagacc tgattaacca cgtcactcac ggcatggtga tcaaatcact tgccacacag
    10741 caacttagcc cgtttgaagt acgccgctgg cagcaggaat taaacagtcc gaatgagcaa
    10801 attcgccagc tcgccattga tgaaattggc ctgatgctca agcgatttag cctctctagc
    10861 tgggaaccga ttctggtcaa taaaacctcg cgcacacaca aaaacagcgt ctcgcgccat
    10921 gcgcaatttt atgttagcca gatgctgggc tttattgccg aaaactatga tcaggcgctg
    10981 accatcaacg atgtggctga gcacgtcaaa cttaacgcca actatgcaat ggggatattc
    11041 cagcgggtca tgcaattgac gatgaaacag tacattaccg cgatgcgcat caaccacgtt
    11101 cgcgcgttac tgagcgatac cgataaaagt attctcgata ttgccctgac ggcaggcttt
    11161 cgttcaagta gtcgttttta cagcacgttc ggcaaatatg tcggcatgtc gccgcaacaa
    11221 taccgcaaac ttagccaaca gcgccgccag acgtttcccg gctaaagacg atatcagtat
    11281 cagccaaaaa aatagtttag ccgtcgataa taagcagtga aaataattta aaataatcac
    11341 ataacgtatt gtttataaaa ctaaatattt caaaaatgtt tgtttttcac gcgctttaca
    11401 gcccgaaaag gccggaagat acttgcccgc aacgaagatt ccttcataac cgggtaagca
    11461 atgatgaaag tattaattgt tgaaagcgag tttctccatc aagacacctg ggtcggtaac
    11521 gccgttgagc gtctggcaga tgctttaagc cagcaaaatg ttaccgtgat taaatccacc
    11581 tcctttgatg atggttttgc cattctctct tcaaacgaag ccattgactg cctgatgttc
    11641 agctatcaaa tggagcatcc ggacgaacat caaaacgtca gacaattgat cggtaagctt
    11701 catgagcgcc aacaaaacgt gccggtcttc ctgttgggcg atcgggaaaa agccctcgcc
    11761 gcaatggacc gggatctgct ggagcttgtc gatgaattcg cctggattct ggaagatacc
    11821 gccgacttta tcgccggacg cgccgttgcc gcgatgaccc gctaccgcca gcagctgttg
    11881 ccgccactgt tcagcgcgct gatgaaatat agtgacatcc atgaatattc ctgggcagcg
    11941 ccaggccacc agggcggcgt tggttttacc aaaacacccg ccggacgttt ctaccatgac
    12001 tactatggtg aaaatctgtt ccgcaccgac atgggcatcg aacgaacttc cctcggttct
    12061 ttgcttgacc atactggcgc atttggcgaa agcgaaaaat atgccgcacg cgtatttggt
    12121 gccgatcgct cctggtcggt agtcgtcggt acttccggct ctaaccgcac catcatgcag
    12181 gcttgcatga ccgataacga tgtcgtggtc gttgaccgta actgccataa atccatcgaa
    12241 caaggtttga tgctgacagg cgcgaaaccg gtctatatgg tgccaagccg caaccgctac
    12301 ggcattatcg ggccaatcta tccgcaggaa atgcaacctg aaaccttgca aaagaaaatc
    12361 agtgaaagcc cgctgaccaa agacaaagcc gggcaaaaac cgtcttactg cgtggtaacc
    12421 aactgcacct atgacggcgt gtgttataac gctaaagaag cgcaggatct gctggaaaaa
    12481 acctccgatc gtctgcactt tgacgaagcc tggtacggct atgcacgttt caacccgatc
    12541 tatgccgatc actatgccat gcgcggcgaa cctggcgatc acaacggtcc taccgttttc
    12601 gccacccact ccatccacaa actgctgaat gcgctgtcac aggcttctta tattcatgta
    12661 cgtgaaggtc gtggggcgat taacttctcc cgcttcaacc aggcctacat gatgcatgcc
    12721 accacctccc cgctgtatgc catctgcgca tccaacgacg tggcggtgtc gatgatggac
    12781 ggcaacagcg gcctgtcact gacacaggaa gtgattgacg aagcggttga tttccgtcag
    12841 gcgatggcgc ggctatataa agagttcacc gctgacggta gctggttctt caaaccgtgg
    12901 aacaaagaag tcgtcaccga cccacaaacc ggcaaaacct atgactttgc tgacgcacca
    12961 accaaactgc tgaccaccgt tcaggactgc tgggtaatgc atccgggcga aagctggcac
    13021 ggcttcaaag atattccgga taactggagt atgctcgacc caattaaagt cagcatcctt
    13081 gctccgggaa tgggtgaaga tggtgaactg gaagaaaccg gtgttccggc ggcgctggtc
    13141 actgcctggc ttggtcgcca cggcattgtg cctacccgca ccactgactt ccaaattatg
    13201 ttcctgttct ctatgggcgt aacccgtggg aaatggggga ctctggttaa caccctttgc
    13261 tccttcaaac accactatga cgccaacaca ccgctggcgc aggtgatgcc ggaacttgtt
    13321 gaacaatatc ctgacactta cgcgaacatg gggattcacg atctgggtga caccatgttt
    13381 gcctggctga aagaaaacaa ccctggcgca cggttgaacg aagcctattc cggcctgccg
    13441 gtggcggaaa tcaccccgcg tgaagcgtac aacgcgattg tcgacaacaa tgtcgaactg
    13501 gtatccattg aaaatctgcc aggacgcatc gcggcaaact cagttatccc gtatccgcca
    13561 ggaatcccga tgctgctgtc tggtgaaaac ttcggcgata aaaacagtcc gcaagtaagt
    13621 tatttacgct cgctgcaatc ctgggaccac catttccctg gatttgaaca cgaaactgaa
    13681 gggactgaaa ttattgacgg tatttaccac gttatgtgcg tgaaagcgta accactattc
    13741 cgctgaaggc gtaattgttt aaataacatt acgccgcctg gccttaggcc ttttgagtat
    13801 ggcaacgttt tcataaaaat tgctgcaaac aaaaatgtca tactttttgc gcggccccac
    13861 cccgcgcttt tttttgcctg ttatttatcc tgtaaaaaaa tatgtacatg agaaattact
    13921 ataaaaattt gtaatattag taaaactcgt tatttttatg tatgtttata ttcatcatgc
    13981 aattatataa ccatttcccg gtatcgcttt gctttagcga gaactggtct tttattcgca
    14041 ctcaggagta catgtatgag gatttgcagc gaccaacctt gtattgtttt attgactgaa
    14101 aaagatgtct ggataagggt gaatgggaaa gaacctatta gccttaaagc taaccatatg
    14161 gcgttattaa attgtgaaaa taatattatc gacgtctcct ctcttaacaa cactttggtt
    14221 gctcatatta gtcacgacat catcaaagat tacctccggt ttctgaataa agatctctcg
    14281 caaataccag tatggcaacg tagcgctacg cccatactca ccctgccatg cctgacgcca
    14341 gacgtctttc gcgttgccgc gcaacacagc atgatgcccg cagaaactga gtcagaaaag
    14401 gaacgaacac gcgcattatt attcacggtg ctatcccgtt ttctcgacag taaaaaattc
    14461 ctttcattaa tgatgtatat gttacgtaat tgtgtaagtg acagcgttta tcaaattatt
    14521 gaaagcgata ttcacaaaga ctggaatctt agtatggtag ccagttgttt atgtcttagc
    14581 ccaagtctgt taaagaaaaa gttgaaaagc gaaaacacca gttatagcca aataatcacc
    14641 acctgccgca tgcgttatgc cgtaaatgaa ttaatgatgg acggtaaaaa tatctctcag
    14701 gtatcacagt cctgcggcta caacagtacg tcgtacttta tttctgtctt taaagacttc
    14761 tacggtatga cgccgctgca ttatgttagt cagcacagag aacgcactgt cgcctgattt
    14821 ttaaccttaa cgaagagcta tattaataac ggcatcagcg ataacccggt cgataataat
    14881 tcaactatcg aatgcaggcg tatgatatga cgtaatttat tgtcacgaag ctcgccttcg
    14941 caggagttta attatgtctt cggatgctga tgctcacaaa gtgggcttaa tccccgtcac
    15001 cctgatggtg tcggggaata ttatggggtc aggtgttttt ctgttacctg caaacctggc
    15061 ctccactggc gggattgcca tttatggatg gttggtgacg attatcggtg cgctggggct
    15121 ctcgatggta tacgccaaaa tgtcgttcct cgacccaagt cctggtggtt cttacgctta
    15181 cgcccgccgc tgctttggcc cgtttctcgg ttatcaaacc aacgtcctct actggctggc
    15241 ctgctggatc ggcaatatcg ccatggtggt cattggcgta ggatatttaa gttacttctt
    15301 cccgattctg aaagatccat tggtattaac catcacctgc gtcgtggtgc tgtggatctt
    15361 cgtcctgctg aacattgtcg gtccgaaaat gatcacccgc gtgcaggccg ttgccactgt
    15421 gctggcgctg atccccatcg tcgggattgc cgtatttggc tggttctggt tccgtggtga
    15481 aacctatatg gcggcatgga acgtcagcgg cctgggcacc ttcggtgcga ttcaaagtac
    15541 ccttaacgtt acgctgtggt cgttcatcgg tgtggaaagt gcctccgttg ccgcaggtgt
    15601 ggtgaaaaac ccgaaacgca atgtccctat cgccaccatt ggtggggtat tgattgccgc
    15661 cgtttgctat gtgctctcca ccaccgcgat tatggggatg atccctaatg ccgcactgcg
    15721 cgtttctgcc tcgccattcg gtgatgccgc acggatggcg ttgggtgaca ccgctggggc
    15781 cattgtttcc ttctgcgcag ctgcgggttg cttaggttca ctgggcggct ggacgttgct
    15841 ggcgggtcaa acggcgaaag ccgctgccga tgacggactg ttcccaccga tttttgcccg
    15901 tgtaaataaa gcgggtacgc cagtggcggg gctgattatc gtcggtattt tgatgaccat
    15961 cttccagctc agcagcattt caccaaacgc gaccaaagag ttcggtctgg tttcttccgt
    16021 ctcggtcatc tttacactgg tgccatatct ttacacctgt gcggcgttac tgctgctcgg
    16081 ccacggtcac tttggtaaag cacgcccggc atatctggct gttactacca ttgccttcct
    16141 ctactgcatt tgggccgtgg tggggtccgg ggcgaaagag gttatgtggt catttgtcac
    16201 cctgatggtc atcaccgcca tgtatgcgct gaattacaac cggctacata aaaacccgta
    16261 tcccttagat gcaccaataa gcaaagatta attcccctta atccagcaaa cataaaagcc
    16321 aaccttaaga acttaaggtt ggcttaattt tgctttgcga gcatatgcgc actttgttcg
    16381 atggaaacac cgtgatgttg aagcgcctac taaaaagacc ctctttgaat ttactcgcct
    16441 ggctattgtt ggccgctttt tatatctcta tctgcctgaa tattgccttt tttaaacagg
    16501 tgttgcaggc gctgccgctg gattcgctgc ataacgtact ggttttcttg tcgatgccgg
    16561 tcgtcgcttt cagcgtgatt aatattgtcc tgacactaag ctctttctta tggcttaatc
    16621 gaccactggc ctgcctgttt attctgattg gcgcggctgc acaatatttc ataatgactt
    16681 acggcatcgt catcgaccgc tcgatgattg ccaatattat tgataccact ccagcagaaa
    16741 gttatgcgct gatgacaccg caaatgttat taacgctggg attcagcggc gtgcttgctg
    16801 cgctgattgc ctgctggcta aaaatcaaac ctgccacctc gcgtctgcgc agtgttcttt
    16861 tccgtggagc caatattctg gtttctgtac tgctgatttt gctggtcgcc gcactgtttt
    16921 ataaagacta cgcctcgttg ttccgcaata acaaagagct ggtgaaatcc ttaagcccct
    16981 ctaacagcat tgttgccagc tggtcatggt actcccatca gcgactggca aatctgccgc
    17041 tggtgcgaat tggtgaagac gcgcaccgca acccgttaat gcagaacgaa aaacgtaaaa
    17101 atttgaccat cctgattgtc ggcgaaacct cgcgggcgga gaacttctcc ctcaacggct
    17161 acccgcgtgc aactaacccg cggctggcga aagataacgt ggtctatttc cctaataccg
    17221 catcttgcgg cacggcaacg gcagtttcag taccgtgcat gttctcggat atgccgcgtg
    17281 agcactacaa agaagagctg gcacagcacc aggaaggcgt gctggatatc attcagcgag
    17341 cgggcatcaa cgtgctgtgg aatgacaacg atggcggctg taaaggtgcc tgcgaccgcg
    17401 tgcctcacca gaacgtcacc gcgctgaatc tgcctgatca gtgcatcaac ggcgaatgct
    17461 atgacgaagt gctgttccac gggctggaag agtacatcaa taacctgcag ggtgatggcg
    17521 tgattgtctt acacaccatc ggcagccacg gtccgaccta ttacaaccgc tatccgccgc
    17581 agttcaggaa atttacccca acctgcgaca ccaatgagat ccagacctgt accaaagagc
    17641 aactggtgaa cacttacgac aacacgctgg tttacgtcga ctatattgtt gataaagcga
    17701 ttaatctgct taaagaacat caggataaat ttaccaccag cctggtttat ctttctgacc
    17761 acggtgaatc gttaggtgaa aatggcatct atctgcacgg tctgccttat gccatcgccc
    17821 cggatagcca aaaacaggtg ccaatgctgc tgtggctgtc ggaggattat caaaagcggt
    17881 atcaggttga ccagaactgc ctgcaaaaac aggcgcaaac gcaacactat tcacaagaca
    17941 atttattctc cacgctattg ggattaactg gcgttgagac gaagtattac caggccgcgg
    18001 atgatattct gcaaacttgc aggagagtga gtaaatgaaa attctgattg ttgaagacga
    18061 tacgctgtta ttgcagggac tgattctggc ggcgcaaacc gaaggctacg cgtgcgatgg
    18121 cgtgacaacc gcgcggatgg cggaacaaag ccttgaggac ggtcattaca gcctggtggt
    18181 actggattta gggttacccg acgaagatgg actgcatttt ctcgcccgta tccggcagaa
    18241 aaaatacacc ctgccggtac tgatcctcac cgctcgcgat acgctgaccg acaaaatcgc
    18301 cgggctggat gtcggtgccg atgactatct ggtgaagcct tttgcgctgg aagagttaca
    18361 cgcccgtatc cgcgccctgc tacgacgcca taataatcag ggcgaaagtg agctgattgt
    18421 tggcaatctg acgctgaata tgggtcgccg tcaggtatgg atgggcggtg aagagttgat
    18481 tctgacgccc aaagaatatg ctctgctgtc acggttaatg ctcaaagcag gcagtccggt
    18541 acatcgggaa attctctaca acgacatcta taactgggac aatgaaccct cgaccaacac
    18601 cctggaagtg catatccaca atctgcgcga caaagtgggc aaagcccgta tccgcaccgt
    18661 gcgcggcttt ggctatatgc tggtcgcgaa tgaggaaaac taattgaatc tgatgcgttt
    18721 tctgcgccga ccaatatcgc tgcgccaacg gctgatattg accatcgggg ccattttgtt
    18781 ggtgtttgag ctgatcagcg tcttctggct atggcatgaa agtaccgagc agattcagct
    18841 gtttgagcag gcgctacgcg acaatcgcaa caacgatcgc cacatcatgc gtgagatccg
    18901 cgaggcggtc gccagcctga ttgtccccgg cgtctttatg gtcagcctga cgctatttat
    18961 ctgctatcag gcggtacgcc gcatcacccg cccgctggcg gagctgcaaa aagagctgga
    19021 agcgcgcacc gccgacaact taacgcccat tgccattcac agcgccaccc tcgaaatcga
    19081 agcggtggtt tcggcgttga acgatctggt cagtcgcctg accagcacgc tggataacga
    19141 aaggttgttt accgctgacg tcgcgcacga actgcgaacg ccactggcgg gggtgcgttt
    19201 gcatctggaa ctgctggcga aaacgcatca cattgatgta gcaccgttag tggcacggct
    19261 tgatcagatg atggagagcg tctcccagct gctgcaactg gcgcgtgccg gacagtcatt
    19321 ttcttccggt aattatcaac atgtaaaact gctggaagat gtcattctcc cctcgtatga
    19381 cgaactcagt accatgctcg accagcgaca gcaaaccctg ctattgccag agagcgccgc
    19441 cgacatcacc gttcagggtg atgcaaccct gctgcggatg ttattgcgaa acctggtaga
    19501 aaacgcccat cgttacagcc cgcaaggcag caacattatg attaagctgc aagaagatgg
    19561 cggagcggtc atggcagttg aagatgaagg accaggtatt gatgagagta aatgcgggga
    19621 gttgagtaaa gcgtttgtac gtatggacag ccgttatggc gggattggtc tggggttaag
    19681 tattgtcagc cgcattacac agttgcatca cgggcagttt ttcctgcaaa accggcaaga
    19741 gacttccggc acgcgggcct gggtacggct gaagaaagat cagaacgtgg caaaccagat
    19801 ataaagaaag ctgctgacca ccagcacgct gaacacggta gttaaacttt cataaacacg
    19861 gtttttcatt gcactctcct ccgttaacct ggaggagagt atgcgcgtca gagattaagt
    19921 gaaccttaag agttcaattg gcctgataag acagcgtcac atcaggccat ccgtttcagc
    19981 ttattcatca attcgcggat gttgctgcac caggcgggta cgtttcgcct gcaaatcggc
    20041 aatctcgtgg tcaatattat cgattttctg ctcgatatta tcgtaatgct cgacgagaat
    20101 ttccttcgct tcctgtatat ctgacgccgc cggtgtcgca cctttcaacg gacgatttgc
    20161 cgtctctttc atggttacgc cggtgattaa accaatcacc gccactacca tcaggtaata
    20221 ggcaggcatc atcagattct gcgagctttc gaccagccag gccgccagtg ttggcgtcag
    20281 accggcaacc agcaccgaaa tattaaatgc cgccgccagc gcgctgtaac ggatatgcgt
    20341 cgggaacatc gctggcaagg tagaagccat aacgcccata aagcagttaa ggatcaccgc
    20401 cagcatcagt aacccggcaa aaatcaggcc gatgacgtta ctgttaatca gaataaacgc
    20461 cgggatcgcc aacacaaaca gtgcaacact accaagtagc acaaacggac gacggccaaa
    20521 acggtcactc agcaagccca tcaccggctg gacaaacagc ataccgatca taatggcgat
    20581 aataatcagc accccgtggt cttcggagta atgcaggtta tgcgacaaat aactcggcat
    20641 ataggtcagc aacatgtagt aagtcacgtt ggtggcaatt accagaccaa tacatgtcaa
    20701 caggctgcgc cagtatttag tggcaatctc tttaaacgag actttcgggc catcctgcaa
    20761 accttcgcgg tcgccctgtt ccagtttatc aacatgctgc tggaacgccg gagtttcttc
    20821 cagcgcatgg cgcaggtaaa gcccgataat ccctaacggc agagcaataa agaacggaat
    20881 acgccagccc cagtcgagga agttcgcttc gccgacaatg gtcgaaatta acaccaccac
    20941 gcccgcaccc agcacaaacc cggcaataga accgaagtcc agccagctgc ccataaagcc
    21001 acgtttacgg tcaggggagt attccgcaac aaatatcgac gccccggtat attcaccacc
    21061 gaccgagaaa ccttgtgcca tcttacagat caacagcaga atcggtgccc aaataccaat
    21121 cgtgtcgtag gacggtatta agccaataca gaacgtactg atcgacataa tcacaatagt
    21181 gatagcgagg atcttctggc gaccatattt atcgcccaac ataccaaaga agagtccgcc
    21241 aagcggtcga atcagaaagg gaacggagaa agtggcaagt gcagcaacca tctgcacgct
    21301 ggggtcagcc cccgggaaaa aaactttacc taatgcgtaa gcaacaaaac cataaacacc
    21361 aaaatcaaac cattccattg cattacccag tgatgctgcg gtaatggctt tacgcagttt
    21421 accgtcatca ataatggtga catcacgaag ggtaatcggt tttacttttt tccttttcag
    21481 catagctttc ctcgcagagt tggcccattt ccgcaaacgc cagaaaaatc agcgcgacgg
    21541 ccgcaaaagg ggagcgggaa ttgcaggatg caatctctgt aacaagcgta gcgggtttgc
    21601 ttacaccctc cggttaagga atgtacaatc aaaccagttg acgcctgaat tgggcagtta
    21661 atgacctttt taccctacca gtgtttaaat ttgtgatcaa cttcacacat ggatttcgtt
    21721 taggactcat tgatgtaact gattatttac gccaaattta cagagaagat ttcctcgacc
    21781 attgccccca ccaaacgctg cgctgaacag agtcgtggca tccccagcgc gacagtttgc
    21841 cagcgttggg tcactgacca gcaaacgggt tgtttatctg ccgtggtcca ctttcccagc
    21901 caacttaaaa aatcagcatg atccccgccc gtcggaacgg tcgaagaggc aaaccactga
    21961 tggtaatagt gacgcgtagt cggtgccgca ttgatactct ctgccagatg ttgtgtcgct
    22021 ttctcgcgca aatcacccag caacaacacc accgcttgcg ggcttcccag acggcaggca
    22081 tcgccaaatg caccccagcg caactcctcc agaacgataa aacagcgccc aggcaacgac
    22141 caggcgttat acgccccagc acgccagcgg gtaaaaattt gctcgctctg atcacctgcc
    22201 tgcaccagta ggcctcgttg cgttaacgcc ttttccttcc agcgacaacg ggctgaaaac
    22261 cactcataca aggcggcaat ttgctggggg agctccggga aagcagagtg ctggcggtga
    22321 tccatttttt gcagcagcgc cagatgctgc gcgatctgcg tcagcgccag atgaccggga
    22381 tccagcatcg acgccagttt ttccaccatt gtcagtcgca ggctaagcgg cgcatttaac
    22441 atgccgccgt tcgtccatgt ctgaagctgc acacgcgaaa gaagttcctg cattagtcgt
    22501 tcacgaaact gctgctgatg ggcttgagga tggcgggcat catcgccctg cgccagatcc
    22561 actaaaaagc gcggatgaac cgactccagc acgcgaccgg ggccgtccag taacgtcttg
    22621 gtcaatatcg ttctgccgtg aaaagtgttt gaatatcatc gcgtaacagc tgagcgtcgg
    22681 tgtaaatcca gctgtgagtc atcacagtct gctgcaattg ctgctgcatc agcctgacca
    22741 ccgattcatt ttgttgacgc agagccagac tttcgcgtaa acgcgtctgt aattctgtca
    22801 aacatgaagc gaactcagcg aaaaaagtat tcatgcctgc cgtaacagat tcatcgacct
    22861 gctctgccag aactttagcc atttgttggc aatagagatc gacttctgcg cttaatgctc
    22921 gttgcaacac actgtaatca accgtttctg tcggggattt ctcatttccc cgtccccagt
    22981 cgggctgatt caaccagcgc gaaaaagtct cacgcacaac gcctaaacgc gtgctctgct
    23041 cgtccgttgc ttcctggcgc gaaatgactt cactgaacag ctggcgagtg ttgaagtggg
    23101 gaactacgcc gtgaaaaaca ggaaaatgaa acccaggacg aaaccctgac tcgctcaatt
    23161 ccattttgac ttgttgctca atggggcgaa taacatcggt taacacccgg caaagggtgg
    23221 attccatctc ggcaaaacgc agcgtaaagt cgcgactgat ggtgttctgc gccgtctgta
    23281 acagtgtctc acagcgggta cgaatctcgc ttaacggctc cgaatcatcc tgaaacaagg
    23341 cggctaactg cgcattcacc gcatcttgct gttgacgcag aaagtggttg gcggaggtca
    23401 gggccagctc gatttcatgt ttaatctcgc cgctcacctg cgcctgattg agttgcaata
    23461 gctgcaaact ttcttcgacc tgatggatat tttgccgcaa ttgttcacaa gcgacgttta
    23521 acccgtgcgc acgaaaatcc aggtattccc gcgcctgctg cgcgtaattc aacagtttat
    23581 gcgcagcaga tcgcagagca tacaatgagg cgttagcgta agcggcatga agcaacgcct
    23641 gaattggctg ggcgaacagc gaatcttccc acaactgatc ggcagcatga cgaatatgtt
    23701 cgaggtccgc cagatcggca tgacgccagc gcctgccgag cgcggcatgg gcaaaatctt
    23761 ccacccagcg ttgttgctct ggcgctggta acttaccgtt gttggctaac tcatagcgcg
    23821 cccgattcgc caggtagccc cacatcgacg acaccgggaa tatctgctgt ggcgtaatac
    23881 agcctttcat cagcgtcccg gaaatcagtg cccgcacctg gtcggcgtcg tcactgttac
    23941 gatcctgttg atcgaactta ttgaccagca catacagcgg caccgattgc cccaccgcca
    24001 aaatcgcctc acggacctct tcatcggaga tcgatttcag ttgcgtataa tccagcaccg
    24061 ccagtaccgc cgaggcgcgt gccagctgct ggttaagcat tttttgcaga tgcggttgcc
    24121 cggcttcatt tggcccgggg gtatccagta acgtcaactg accgggataa ctttccagcc
    24181 ccgccagatg gacaaactcc acttcaatca cgggaatatg ctcaatggcg gcgtaagcag
    24241 aaaaaggaaa atcgacgtcc agcgccttcg ccagtcgcac taaatcattc aaacttttca
    24301 gacaatgaaa aataggctgg gcacccagat aatatttttc gaaagcgacg ccattttcga
    24361 tccgctgcat aagcgcacgc atatctttat ctatttccag cacatcggtc agatgcttaa
    24421 tatcgcaatc acgcaggcgc tgttgtaatt gttgaattaa acaatcgatt ggcgcgacat
    24481 gtgaaaaatg cagtaccggt tccttttgcc cgggcgtatg gcgaataagc gtcggcagcg
    24541 cagtcattgg gcgattacga ttaggcagaa cctccgtacc aacaatggca ttaatggtgg
    24601 ttgatttccc tgctttcatg gtaccgacaa ttgccagcac catttccagt cgggaaattt
    24661 tacacaactc attattcagc atcgcgtgac gttcggcgat attaggctga ctccagggta
    24721 aagccagttg tggcgcgtcg tctccgggta cagagagagg cattttttcc agtaactgca
    24781 actgttggcg agaaagctgt aacaggcgtt cagcctcctg acttaactca tacagggtct
    24841 gtgtgtacat agaaaattct tccttaaagc aaattttgtt acttttattt agccagattg
    24901 tttttgagtt ctgttttcgg cttttataat tactgcaaga aataatttta tatttagtgt
    24961 gttgtttttt atcagaataa ataacgtctt ctgatgcgtt taaaacgtca gaaagataaa
    25021 aatatcatgt gaataaaaaa aagaacaagt agagcattaa cattatctta aataataaat
    25081 agaggcaaaa agattatttt ctttttacgt ttcctttcaa atgaaaacga tcgtcgtcta
    25141 aaatcagcag tacccccgac aaactcaggg attttgtgta taattgcggc ctttttcggc
    25201 aatctgccgt tttttggcgc ttttgccctg ctgacttttg aggaaatcca catgtcatta
    25261 ccacactgcc caaaatgcaa ctccgaatac acttacgaag ataacggcat gtacatctgc
    25321 ccggaatgtg cctacgaatg gaacgacgca gaacctgcac aggaaagcga cgagctgatc
    25381 gttaaagatg ctaacggcaa tctgctggct gacggcgaca gcgttaccat cattaaagat
    25441 ctgaaggtga aaggtagctc ttcgatgctg aaaattggca ccaaagtgaa aaacatccgc
    25501 ctggttgaag gcgaccataa catcgattgc aaaatcgacg gttttggtcc gatgaaactg
    25561 aaatctgagt ttgtgaaaaa gaactgattg tattgtgatc ggtaagccgg ataaggcgct
    25621 cgcgccgcat ccggcaacga tgccgactgc ctgatgcgac gcttgcgcgt cttatcaggc
    25681 agcctctcat ctgtataaat ttcgaactac acttaactgg cttctcttaa ctgaggtcac
    25741 catcatgccg ttaagtccct acctctcttt tgccggtaac tgttccgacg cgattgccta
    25801 ttatcaacgt acgttgggcg cggaactgct ctataaaatc agcttcggcg aaatgccgaa
    25861 atcagcgcag gacagcgccg agaactgccc ttccggaatg caatttcccg ataccgccat
    25921 cgctcatgcc aacgtgcgca ttgccggaag cgacatcatg atgagcgatg ctataccgtc
    25981 aggaaaagcc agctactccg gctttacgct ggtgctcgat tcgcaacagg tcgaagaagg
    26041 aaaacgctgg tttgataacc ttgccgctaa cggaaaaatc gaaatggcct ggcaggaaac
    26101 tttctgggcg catggctttg gcaaagtcac cgataaattt ggcgtaccgt ggatgattaa
    26161 tgtcgtcaaa caacaaccaa cgcaataacc cgccgggagg cccgccctcc cgcactgtca
    26221 tcgaattccc gttaactctt catctgttag tcacttttaa ttaaccaaat cgtcacaata
    26281 atccgccacg atggagccac ttttttaggg aggctgcatc atgcaaacga ttatccgtgt
    26341 cgagaagctc gccaaaacct tcaatcagca tcaagcgctg catgcggttg atctgaacat
    26401 tcatcacagt gaaatggtgg ctctgcttgg gccgtcgggt tccggcaaat ccaccctttt
    26461 acgtcattta agcggtttga ttaccggcga taaatccgcc ggtagccata tcgagctact
    26521 gggccgcact gtccagcgcg aaggccgcct ggcccgcgat atccgcaaaa gccgcgccaa
    26581 caccggctac atctttcaac aattcaacct ggtgaaccgc ctgagcgtac tggagaacgt
    26641 gctgattggc gcgctcggca gcacgccgtt ctggcgcacc tgttttagct ggttcaccgg
    26701 cgagcagaaa cagcgcgcgt tacaggcgct gacccgcgtt ggcatggtgc attttgccca
    26761 tcagcgcgtt tccaccctct ccggcggcca gcagcaacgt gtggcgattg cccgtgcgct
    26821 gatgcagcag gcgaaagtga ttctggccga tgaacccatc gcctcgctgg acccggaatc
    26881 agcgcgcatc gtgatggaca ccctgcgcga catcaaccag aacgacggca tcaccgtggt
    26941 cgtcacgctg catcaggtgg attacgccct gcgctactgc gaacgcatcg tcgccctgcg
    27001 tcaggggcac gtcttctacg acggcagcag ccaactgttt gataacgaac gttttgacca
    27061 tctctaccgc agcattaacc gcatcgaaga gaacgcgaaa gctgcctgat atccccatca
    27121 ttgaggaaaa cgaatgaacg ctaagataat tgcctcgctg gccttcacca gcatgttcag
    27181 cctcagcacc ctgttaaccc cggcacacgc cgaagagcag gaaaaggcgc tgaatttcgg
    27241 cattatttca acggaatcac agcaaaacct gaaaccgcaa tggacgccgt ttttacagga
    27301 tatggagaag aaactgggcg tgaaggtcaa cgccttcttt gccccggact acgcgggcat
    27361 tatccagggg atgcgcttca ataaagtgga tattgcctgg tacggcaacc tgtcggcgat
    27421 ggaagcggtg gatcgcgcca atggccaggt cttcgcccag acggtcgcgg cggatggatc
    27481 gccgggttac tggagcgtgt tgatcgtcaa caaagacagc ccgatcaaca acctgaacga
    27541 tctgctggcg aagcggaaag atctcacctt cggcaatggc gatcctaact ccacctctgg
    27601 cttcctcgtc cccggttact acgtcttcgc taaaaacaat atctccgcca gcgacttcaa
    27661 gcgcaccgtc aacgccgggc atgaaaccaa cgcgctggcc gtcgccaaca agcaagtgga
    27721 tgttgccacc aacaacaccg aaaacctcga caagctgaaa acctccgcgc cggagaagct
    27781 taaagagctg aaggtgatct ggaaatcgcc gctgatccta ggcgatccga tcgtctggcg
    27841 caagaatctg tccgaaacta ccaaagacaa gatctacgac ttctttatga actacggaaa
    27901 aacgccggaa gagaaagcgg tgctggaacg cctgggctgg gcgccgttcc gcgcttccag
    27961 cgacctgcaa ctggtgccga ttcgccagct cgcactgttt aaagagatac aaagcgtgaa
    28021 aggcaataaa gggctgaatg agcaggacaa gctggcgaaa accaccgaga ttcaggcgca
    28081 gctggatgac ctggaccgcc tgaacaacgc gttaagcgcg atgagttcgg tgagtaaagc
    28141 ggtgcagtaa atcgtaggtc ggataagacg ccccggcgtc gcatccgaca atgtgtaggc
    28201 gttgatgccg gatgcggtgc aagcacctta tccggcctac agaccggagc caaacatgca
    28261 aaccatcacc atcgccccac ccaagcgtag ctggttctcg cttctgagct gggccgttgt
    28321 tctcgacgtg ctggtcgtct cgtggcaggg cgcggaaatg gccccgctta cgctgattaa
    28381 agacggcggc aacatggcaa cctt
//
LOCUS       DAJTQY010000051        28341 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.051, whole genome shotgun sequence.
ACCESSION   DAJTQY010000051 DAJTQY010000000
VERSION     DAJTQY010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28341
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.051"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(19..1014)
                     /gene="yjfF"
                     /locus_tag="OO349_002246"
                     /pseudo
     CDS             complement(19..1014)
                     /gene="yjfF"
                     /locus_tag="OO349_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
     gene            complement(1001..2023)
                     /locus_tag="OO349_002247"
     CDS             complement(1001..2023)
                     /locus_tag="OO349_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5867301.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEVVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSHDKT"
     gene            complement(2037..3539)
                     /locus_tag="OO349_002248"
     CDS             complement(2037..3539)
                     /locus_tag="OO349_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCR5867302.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQTISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEMELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDELTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAQLSVPAIMNAIAA"
     gene            complement(3679..4635)
                     /gene="ytfQ"
                     /locus_tag="OO349_002249"
                     /pseudo
     CDS             complement(3679..4635)
                     /gene="ytfQ"
                     /locus_tag="OO349_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
     gene            4945..5475
                     /gene="ppa"
                     /locus_tag="OO349_002250"
     CDS             4945..5475
                     /gene="ppa"
                     /locus_tag="OO349_002250"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HCR5867303.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVAVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(5854..6195)
                     /locus_tag="OO349_002251"
     CDS             complement(5854..6195)
                     /locus_tag="OO349_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HCR5867304.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(6198..9977)
                     /gene="tamB"
                     /locus_tag="OO349_002252"
     CDS             complement(6198..9977)
                     /gene="tamB"
                     /locus_tag="OO349_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HCR5867305.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQLGVAVKAGNLHLGVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVVEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGNADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTLGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(9974..11707)
                     /gene="tamA"
                     /locus_tag="OO349_002253"
     CDS             complement(9974..11707)
                     /gene="tamA"
                     /locus_tag="OO349_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HCR5867306.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQHVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFVGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            11913..12551
                     /gene="msrA"
                     /locus_tag="OO349_002254"
     CDS             11913..12551
                     /gene="msrA"
                     /locus_tag="OO349_002254"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCR5867307.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            12874..14217
                     /locus_tag="OO349_002255"
     CDS             12874..14217
                     /locus_tag="OO349_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCR5867308.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(14279..14485)
                     /locus_tag="OO349_002256"
     CDS             complement(14279..14485)
                     /locus_tag="OO349_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HCR5867309.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDIGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            14810..15364
                     /locus_tag="OO349_002257"
     CDS             14810..15364
                     /locus_tag="OO349_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HCR5867310.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFEIGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTPEEVQQVMDLLQKLLK"
     gene            complement(15427..16365)
                     /locus_tag="OO349_002258"
     CDS             complement(15427..16365)
                     /locus_tag="OO349_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5867311.1"
                     /translation="MLPRIRHNNFIGAVELFVKSSHTKTHSNDFFNNIQHAFKKKDWV
                     SNYDSLLTLRESFRCATQIDKNSYQVLSSKNETVNAMDNFLISFSLKDNGAEYTITLR
                     GSGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYACDILQKKQSDIPKRIKGYIRQ
                     AFGDVSFGYALLEDVVSKLKRGKFELQIPGGGIKECDGWYIYEKIIDDNFAIVIESLG
                     FALKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADS
                     NITKEKICEKYKSTIEAFKKEQLALPVLVRCKNSET"
     gene            complement(16577..17317)
                     /gene="cysQ"
                     /locus_tag="OO349_002259"
     CDS             complement(16577..17317)
                     /gene="cysQ"
                     /locus_tag="OO349_002259"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HCR5867312.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLAPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKLIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            17507..19450
                     /gene="cpdB"
                     /locus_tag="OO349_002260"
     CDS             17507..19450
                     /gene="cpdB"
                     /locus_tag="OO349_002260"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HCR5867313.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VIVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIAPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(19576..19956)
                     /locus_tag="OO349_002261"
     CDS             complement(19576..19956)
                     /locus_tag="OO349_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCR5867314.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            20045..20905
                     /gene="qorB"
                     /locus_tag="OO349_002262"
     CDS             20045..20905
                     /gene="qorB"
                     /locus_tag="OO349_002262"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCR5867315.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYSDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYATAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGCPTTTLAE
                     SVSHLFNVNN"
     gene            21014..21979
                     /locus_tag="OO349_002263"
     CDS             21014..21979
                     /locus_tag="OO349_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR5867316.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATVQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            22087..22749
                     /gene="ytfE"
                     /locus_tag="OO349_002264"
     CDS             22087..22749
                     /gene="ytfE"
                     /locus_tag="OO349_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HCR5867317.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(22794..24206)
                     /gene="cycA"
                     /locus_tag="OO349_002265"
                     /gene_synonym="dagA"
     CDS             complement(22794..24206)
                     /gene="cycA"
                     /locus_tag="OO349_002265"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HCR5867318.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHVQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(24514..25134)
                     /gene="fklB"
                     /locus_tag="OO349_002266"
     CDS             complement(24514..25134)
                     /gene="fklB"
                     /locus_tag="OO349_002266"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR5867319.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            25353..25898
                     /locus_tag="OO349_002267"
     CDS             25353..25898
                     /locus_tag="OO349_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OapA family protein"
                     /protein_id="HCR5867320.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNASVVTREAQLDIQSQSQPPTEEQPQTTQTQPFQPDSGIDNQWRSYRV
                     EPGKTMAQLFRDHGLPATDVYAMAQVEGAGKPLSNLQNGQMVKIRQNASGVVTGLTID
                     TGNNQQVLFTRQPDGSFIRAR"
     gene            complement(25882..26529)
                     /locus_tag="OO349_002268"
     CDS             complement(25882..26529)
                     /locus_tag="OO349_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR5867321.1"
                     /translation="MYIDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFGNKENLFIEVLEYTWNDIRQKEKALDFSDLPPQKAIEKIIDFTWDYYIANP
                     WFLKIVHSENQSKGVHYAKSQRLLEINHAHLQLMESLLDEGKKYNIFKPGIDPLQVNI
                     NIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILSWLLVDPSSTAHE"
     gene            26741..27532
                     /locus_tag="OO349_002269"
     CDS             26741..27532
                     /locus_tag="OO349_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HCR5867322.1"
                     /translation="MKSTRPVAVITGATRGIGKGCAQELARGGFNLLINDRPDADSVE
                     KLHITQQECLAEGVEVICFPADVGDLSLHEEMLDAAQNQWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            27542..>28341
                     /locus_tag="OO349_002270"
     CDS             27542..>28341
                     /locus_tag="OO349_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCR5867323.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQGEDLQQITRQLAASNMSVSGLCRSTYYTAPTLAERKLAIDDNRRALDDAAVLNAA
                     CYMQVVGGLPTGTKDLYEAREQVKQGIRQLLPHSKDVGVPIALEPLHPMTAADRSCLC
                     TLRQALDWCDELDPDGEFGLGVAVDVYHVWWDPDLASQILRAGKRILAFHVSDWLMPT
                     TDLVNDRGMPGDGVINIPSIRRLVENAGFNGAIELEIFSPYWWQKDIN"
BASE COUNT         6954 a         7543 c         6989 g         6855 t
ORIGIN      
        1 ccacacggag aggcattttt atcgctgcgc aatgttgact cttgtcactg gcgaactctg
       61 acgattctcc cacagcaccg ttaacccacg ctgtaatgct ataaaaataa acaacaaaat
      121 accgatggcg attttcgtcc actaggaact cagcgtgccg tcaaagttga tgtaagtctg
      181 gatcagtccc tgaattgcca cgccaaaaag cgtcccaagc accgtcccga cgccaccact
      241 taacaacgta ccgccaatca ccaccgacgc gatggcgtcc agttccacac ctacgcccgc
      301 cagtgcatat ccggcttggg tataaatcga gaagacaatc cccgccagtg ttgccagtcc
      361 ggtggagagc atataaatgc gaatagtggt gctgcgagta gaaatcccca tcaggttcgc
      421 cgacgtcgcg ttgccgccaa tagcgtacac ctgattgcca aaacgggtac aatgcgcaag
      481 gaaaataccg ataaccacca ccgccagcat caacagcccc atcgcactaa ggcgaccgcc
      541 gccggggatt ttccacgcaa ggcttgagag cgtgtcataa atgggatggt ttatcggaat
      601 cgactcttcc gaaacgagat agctgacgcc gcgcaaaaag aacatccccg caagcgtaat
      661 gataaatgcc gggatcttca gggcgtcgat cagaagcccc ataaatgcgc cgaaggcaca
      721 gcccatcacc agcactagcg gaaacgccag cagcggcgag aggccgaaat cgccaaccac
      781 ttttgccaga aacacgccag taaaggcgat caccgaaccg acggagagat cgataccgcc
      841 agagaggatc acaaaggtca tgccaacggc gattatccca agaaaggcgt tatcggtcag
      901 gatattgcag atcacccttg tggaagcaaa accgggaaac tgggtcaggc agtagagata
      961 gcccagcaca aagacgccga tggtgatcat cagcggcaag ttacgtttta tcatggctac
     1021 gtactccttt aatcagactg ataaagcgtt gcgactgaac aatcagcaca caaagcacca
     1081 ccaccgcttt caccacctgg ttcatctccg gcggaaagcc cgaaagcaaa attccggtgt
     1141 tcatcccctg aataatcagc gcccccacta ccgagagcag caggttaaag cgcccgccca
     1201 tcagcgatcc gccgccaatc accaccgcga gaatggcgtc cagctccagc cataatccgg
     1261 cgttgttggc atcggcaccg cgaatatccg ccgccacgat aatgcccgca atcgccgcac
     1321 atagcccgct caacacgtag gtgagcatga cgatgattcg cgtgtttacc ccggcatttt
     1381 ttgccgcccg aatgttgata ccaacgactt cgataaacat ccccagcgcc gttttgcggg
     1441 tcaacagcca gaacaggaga agcgtcagca ccgcgataat gaccggcgtt ggcaggaaca
     1501 acagcgatcc gctgccaaac catgagagat ccggcgagtt aaacgtgacg atctgcccgg
     1561 cggtgatcag ttgcgccacg ccgcgcccgg cgaccatcag gatcagggtg gcgacaaacg
     1621 gctgaatttt gaggatcgct accagtatgc cgttccacaa tcccgccagg atgccggtgc
     1681 ccagggcgct taacaaaaca atcggcaggc tgaatcccgc gaccgtcatc gcagccgttg
     1741 tggctccggc gatagccatc accgccccca cagagagatc gataccaccg gtggcgatca
     1801 ccagcgtcat accaatcgcc agtaacgcaa cgggagctgc acggttaaga atgtcgatag
     1861 ggctaccgaa caaacgccca tcctgaagca ccacctgcca gaaatgcggg gctaccaggc
     1921 tatcgaccag cagcaccagc aacagtgccg ccagctgcgg catccccgtc ggccagtgaa
     1981 aacgcctttt tggcggcgta gtgtcaggga gagattgagg catcacactg ttctccttac
     2041 gccgcaatgg cgttcatgat cgccggaacg gaaagctgtg ccagcgggat ctccgccacc
     2101 tgtttgcgat cgcgcatgat gatcacccgg tcggcatagc ccaccagctc ttccagttcg
     2161 gaggagatca ccagcagcgc cagaccgtca gcacacagcg tttcaatcag gcggatgatc
     2221 tcggcgtggg caccaacatc aatgccacgg gttagctcat caaggatcag aaattgcggt
     2281 cgggtcagta gccaacgtga aagcagcact ttttgctgat tgccaccgga gagaaattca
     2341 atcggttgtt cagttgaagg tgtgcgaatg ccaagctggc ggataaagcg ttcggcaatc
     2401 tcttgctgct ctttgcggga aatgggtcgt agccagccgc gctgggcctg gagagcgagg
     2461 atgatatttt cccgcaccga ggcggcggcg atgatgccat cggttttcct gtcttccggg
     2521 cagaatccga tgcccagtac tgaagcctga tgtggcgatc gcagggtttg cggtttgcct
     2581 ttgatcaacg ccgtgccgct gtcagcaggt ttgataccga agatcacttc ggcggtttcg
     2641 gtacgtccgg atcccagcaa gccagccaga ccgacgatct cgccggggcg cacttcgaga
     2701 tcaaacggtg ctatcgttcc ttttttgccg taatttttga acgcggcaac gggtttgtcg
     2761 ctcaacaatg ttcgcccggc acgctgtagc gcgtgggtat ccagctcgcg ccccagcatc
     2821 atttttacca gttcgatctg cggtagctcg cgcgtttccc gacagcctac gaaactgccg
     2881 ttgcgtaaga cggtgatccg atcgctgacc tgatagacct gatcgagaaa gtgagtgaca
     2941 aagatcaggc tgacgccgcg atcgcgcaac tgacgcatca ggccaaataa tagctccatt
     3001 tcctgggtgt cgaggctggc ggtgggttca tcgaggatca gcactttggc ggagagatcg
     3061 atagcccggc aaatggcgac gatttgctgc atcgccaccg aaaaacggtt aagcggttcg
     3121 cgcacgtcga gggagaaacc gtaagatgcc atcagttcgg tggcgcgctt ttccatctct
     3181 ttacggcgta gaaggccgaa acgtttgggt tcgcgaccta tgaatagatt atcagcaacc
     3241 gacatattgg gtagcaggtt gacttcctga tagacggtgc cgataccgag ttgttgtgca
     3301 tgggcggtat ttttcggtga gatagtctgg ccttccagcc agatggtgcc gcgatcggcg
     3361 tggtatacac cagttaatgc tttgattagc gttgattttc ccgccccgtt ttcaccaagc
     3421 agcgccatga tttcgccacg gcgcaggctg aaatcaacgt tgtctaacgc tttgacgccg
     3481 gggaaaaatt tacttaatcc ttcggtgcgg aggatctcct ggtgttggtc ggtcgtcatg
     3541 gtttcccccg gtgtaaagcg tgctcccagt aggcatgata agacgcggta agcgtcgcat
     3601 caggcttcag tgcaccatcg ccggatgcgg cgcgagcgcc ttatccggcc tacgaatcgg
     3661 catcaggcat agcaaccctc aataccccat atttttcttc ttctctaact cttcttttgc
     3721 ggtatcaggc aggtaaaggg tggatttggt taacgtcagc ttttcaggca tggtgccgtc
     3781 ttttttgtat ttctccagcg catcgaaagc gggtcccgcc atgttcggcg tcagttcaac
     3841 actggcgttc gcttcgccat ccatcatcgc tttgtagatg tccggtacac cgtcaatgga
     3901 acccgtgagg atatctttgc ccggtttcag gcccgcttct ttaattgcct gaattgcacc
     3961 aatcaccatg tcgtcgttat gggcgtaaac catgcagatg tttttgctgt tgttttccgc
     4021 tttgataaag ctctccatga cttctttgcc tttactgcgg gtgaagtcac ctgactgcga
     4081 gcggataatt ttgatatttg gcgcattctt aatggcttcg gcaaagcctt tcttacggtc
     4141 aatggcgacg ctggccccaa cggtgccctg cagctccacc acgttgcatg gtttgccatt
     4201 cacttctttt accagccagt caccgatcaa cttaccttcg aggatgttgt cggcagtaac
     4261 ggtggtcata tagagagatt tgtctttcac atcgatggaa cggtcgagca agaagaccgg
     4321 gatttcggca tctttcgcct cttttaatac cggttcccaa cccgtcgcaa ccactggagc
     4381 gataaagatc gcatccaccc cttatgcaac gaaggaacgt accgctttaa tctggttttc
     4441 ctgcttttgc tgaccatcgg caattttcaa cgtaattccg cgtttttcgg cctcactttt
     4501 cgccacattg gtttctgcgg cgcgccagcc ggattccgat ccgacctgcg aaaatcctac
     4561 ggttaatgga gcggccaacg ccatagacga catggctgcc gagactgcag agactacaag
     4621 taagcgtttc cacatagggt tgtcctcgtc ggggtaatta ttattggtga aaagatgttc
     4681 gcgaaaaaac tatagacaat tcgttatgta acggattgcg ttacatcaca cttcaaaaaa
     4741 gtaaataaaa cgttaatcac aagtttgtaa tcgctttcat ctcactatga aaaatgcggc
     4801 tacggttatg gattttcctg ctctgtatac cgtcttaaaa ctggcgaaaa aggaaaatga
     4861 agacgaaaac aagcaaagac attcggcgcg agttggctat aatacttggc acttgtttgc
     4921 cacatatttt taaaggaaac agacatgagc ttactcaacg tccctgcggg taaagatctg
     4981 ccggaagaca tctacgttgt tattgagatc ccggctaacg cagatccgat caaatacgaa
     5041 atcgacaaag agagcggcgc actgttcgtt gaccgcttca tgtccaccgc gatgttctat
     5101 ccgtgcaact acggttacat caaccacacc ctgtctctgg acggtgaccc ggttgacgta
     5161 ctggtcccga ctccgtaccc gctgcagcct ggttctgtga tccgttgccg tccggttgcc
     5221 gttctgaaaa tgaccgacga agccggtgaa gatgcgaaac tggttgcggt tccgcacagc
     5281 aagctgagca aagaatacga tcacattaaa gacgtaaacg atctgcctga actgctgaaa
     5341 gcgcaaatcg ctcacttctt cgagcactac aaagacctcg aaaaaggcaa gtgggtgaaa
     5401 gttgaaggtt gggaaaacgc agaagccgct aaagctgaaa tcgttgcttc cttcgagcgc
     5461 gcaaagaata aataagttct tctagcgcaa taaccctgaa cgccgggctt cggttagtaa
     5521 gggttttttt atgcccgcga taaataaact ctctattcca ccatcattat tctcagcggt
     5581 tgcaaggctt gaacggtaag aacaagcaaa cccgaccacc attttgctgt tcatagccac
     5641 ttgctggaag ttagccgacc tcactcatac tcaccgttca aagcgtgccc ccgttttacc
     5701 cctgcacatt tgtgaacgcc agcagagttt tttatgcgat tgcagatatg gaaagcggct
     5761 cggagagaaa agcaaaagat ggaaaagtta caaaaaaaat cgcgagatac atcgcataaa
     5821 acaacgccac ccgaaggagg cgtgctttta ttattactta tccctgtcta accagtcgcc
     5881 gctttcaatt agctttaatc catcgacggg tcgttgataa acgtacatcc atgcactccc
     5941 atacggcgtc tgaatcaact ggcgcgcgta ttcaccgccc ctggtgcgca aggcatcaag
     6001 ttcggccagc gtggcgttgt caatacgata aacttcaccg tgtaccgttc cgttccccgg
     6061 aactgcgcct ggatagtggc ccaggctata caactggtag ttatcgatac tgaaatcgcc
     6121 cagtaactgg gcattggtca tccagtgact gttgccttgt ttgtggcgta aactgccgta
     6181 gacaaatatt cgcattgcta aaactcgaac tgatagagca aatccagtgc ctggtctaca
     6241 ccagacacgg cttccagata tagcttaggc atcaggcgat aacgtaacgt gagtgttgct
     6301 atagagtcaa atatacccac gccgtatttc acttgcagac ctggcaatac atagccgctg
     6361 accactacct gggaggagtc gcctactccc agggtgtcga gcgctaaatt gcttacgcca
     6421 aacgtctcgc cgattttacc cacaatctgg ccactttgcg caacccccag accaatcagc
     6481 atcgaggtca ttgccgcact gtcgctctga tcgctctcca gcccttgtcc acgtagcaaa
     6541 taagacaatg cagcttgttg cgacatcgcc gggtcagaga agatctccgc tttcggttcg
     6601 tccgccagac cagtgacgcg aactccggcg attacatcgt cttctgtagc atccgggtta
     6661 cgaatagctt caatattaag atacggttga tccggcggac cagagaacag taactcaccc
     6721 ttacgcacaa tcagatcctg accataggca tggaagcgcc cttcagggat gttgatctgc
     6781 ccgttcaggc ccagcccttg tttgtcctgt acgacgttaa gatcgcccgt cagccgcgct
     6841 ttcaggccaa aggcgtcaat gcgcacattg ttgccaacgt ggacaatcag gttactgtta
     6901 atcggaatcg acgccgtttt cggctcttcc ggttgcaggt tatcgttaag catcaccaca
     6961 tcgctggaga cgcctactgc gctttccggc agatcgtgca ccacgatgcg cgcccacggg
     7021 acatccacgc gaccatcgag ggtaaacagg tttggtgtag cctcgaatac aacatctggc
     7081 gatacatcca ttcgtaccat cggcggcacg gtgatccgca ctttactgcc tttcgccgtt
     7141 actcgtgccc gccagttttc aatttggctc cagtcggcat taccgttcag gtagatttca
     7201 ccctgctggg tccgtactgt accggcaagc gtcgagcgca taccgttaaa gttgaccgca
     7261 agctggctcg gctgcatatc aaacggcata aagttgccgt cgatatccac acccgtaacc
     7321 tgaagctgac caaacaactg cgggctttgc acatcaccac ccagacgcaa gttggcactc
     7381 accatccccg ctgctttttc cccacgggta aagatggggt ttatcatcgc aaggttgaag
     7441 ttacggatat tgacgttgcc accaagatta cggcggcctt gcggatcggt cacctgcacc
     7501 tgtccatcaa actggccgtt attggtcagg cggatggtcc agcccaattc ggcacggttg
     7561 ttacgcaatt ccgccgtcag attcagtgtc tgaaacgcca ccggcagcgc cgcatcgttg
     7621 acggtttgcg ttacctgcac gttacgcccc gaaagggtga tactgccctg cggcagcccc
     7681 tctttcgtgg tgtcccaggc aacatccgct ttacccgtga agataccgct ggcctgagtg
     7741 gtttctggca taaacggttt cagcatggcg aggtcgaagc ggttgagatt caccaccgca
     7801 cgcccttcgg cccccgcatc gatagtttgc ggcacgcaca gttccgcatt cgggttaagc
     7861 cagcagtgtg gcccgatgct gattttttgc tccttattgc ggtaatccag cgcaatatcg
     7921 cgggtcagcg accacgggcc gaccggcgtc tggaagcggg tattgctaag agttcccttc
     7981 cagcgttctt ctttgcgatc aaaacttcct gccagattaa gctgcccgga gacaggctcg
     8041 ccctgaatcc gcaactgtag ctcgtgctgc ttttcgctgc ctttggcatt cagggtgacg
     8101 aggttgatat ttacatccgg ctgcgaaatt tgctcaacgc gtacgtcgag tttcccggcg
     8161 atctgatcgg tggatttaat gtcgccttcc acgcgaacct gcgccacgga aagttcctgc
     8221 cagcgcaggc cgcgcgcggt gatatctgcc agtagttgtg gcgcttccac cgtgccgcgt
     8281 actttcacca gccctttcgc tgtaccgcca agccccggca gcgcgttatc cagccccggc
     8341 gcgttgatgg tggcatcaag attgagatct tttaccccca gctcgccttt cacttcggca
     8401 ctgtttggcc cgagttccag atgaagccca gggatcatcc actgcatata actgttgcct
     8461 ttcagcgtgc cgtcaacgtt cactttgttc tgtttgacgt taccggtcag cttcagttct
     8521 ggcacctcca tctgccaggt gccgccgtac aggctaccgc gggttttaat caagccattg
     8581 agtttcgacg gccactccgg gaactctttg gcggtgttaa tgccattaag cgttagctca
     8641 ccgcgccaac taatggcctg ctgccagtcg agcaacgcct tgagttcagt tttcccttcc
     8701 agcgccgcga cggtgagttt gtcgagattg acctgctgtt cattaccttt ggcatcaagg
     8761 gtaatggtgg ctggcgggat ctcctgtccc ttcactgccg tacgcataga gagcgtgtaa
     8821 tcggtcattt tgccggtaag tttcagtttc agatcatccg cctgatactg cttctcacca
     8881 gtgaacggcc agtaaagctg tttgctgttc acttccacgt tgagtggcaa tccggcttcc
     8941 gccagtcgcg tctgggcgcg taaatccata tccaccggac cggaaaggtt aacgcctatc
     9001 tccagctgtt cgcgcagcgc gccgcccact ttcagcttca ccttttcacc tttcaacggc
     9061 tccacgttca gtgtgctgtt gagggtgata tccaccggcc agttgtctga cagctgcgcc
     9121 gtgccgctgg cgttgacgat cccctggttg gaatcgatat ccagggcgtc cagtttagta
     9181 ttgccgtcaa tgctgctcac tttcagcagc atggtgcgca cggtgatgtc cgtgtcgccc
     9241 gtcacgcgca gctgctcgcc cttaaactct tcaatgttca ggttaagcgg caaatgcacg
     9301 tcggtcattt ccggcaatac cgggcgagaa aagagatctt tcagcgtttc gccgagcggc
     9361 ttttcctccg gctgcggatt ttcaattttc ggttcgacca cttcttcctg cgccacttcc
     9421 accactttcg gcagagcaat cagcaggcct ttcagcgacg tcggtttcag ggtcagggtt
     9481 ttctcctgcc agttcaggcc ggaggtgaag tccatcaccg acaccgtggt gtcatcaatc
     9541 ttgatgttga cgttgtccag cgccacccgt gtcagggtga tgggatacgg cgtggagaga
     9601 tccagcggac cgctatcttc ctcttcttca acctgttcag aaggaggcat ttttttactg
     9661 tcgatgttga cctgaatgtc tttcagcgcc aggtcattaa tacaaacgct actgttccac
     9721 aggcactcaa gaccgacccc cagatgcaga ttacccgctt ttaccgccac gcctagctgc
     9781 tcataacgaa cgtcagacaa ggtgagatcg cgccagccgc cggtgacctt gccaatatcc
     9841 agtcctggca cccagcgatc cgccgcttta aatacaagat gcaggccgct ggtggtgccc
     9901 accagaaacg ccaccgatcc cagcaacagt aagataacga taaccacgcc gaggctgatt
     9961 tttttccata aactcataat tctggcccca gaccgatgta aaactgtaac ccgtgttcgt
    10021 ctttatccgc gaccggtacg gcaaaatcga gtttgattgg cccgaccggc gattcccagc
    10081 gtacgccgac cccggtaccg gttttaaagt cgctgcggcg aatatcgctt accgcttcgc
    10141 cactatcgac aaacaccgcg ccccaccatt ttccggtcac gttgtactgg tattccagcg
    10201 atccggttat caacttcgag gcccctttca ggtcaccgtt ggcgtatttc ggggcgatag
    10261 atttgtattt gtagccgcga atactgcgat cgcccccgac gaagaaacgc agatccggcg
    10321 gtactttgtc gaaatcaccg gtttcaatcc agcccagcgt gccgcgtgtc acaaagcgat
    10381 ggcgatcgta cagtgtgcgg atccagacgt gctgcgcctg gaaaacggag aaatcgacat
    10441 ctgaacccca ggccgtgttg gagtagtcga tagagtagcg ttgcgagtct ccccaggttg
    10501 gcatcaggcc accacgagaa cgcgtgcggc taatcatcac cccaggataa aacagcatcg
    10561 tggtgttggt aatttcaccc tgggtaaagt ggtcgaggct ccagcgcagg ttaatggcac
    10621 gctgccagcc gctggagaga tcccagtagc gagaagccac caacgtagtg gagtcagatt
    10681 cggtatcgtt caggtcagtg cgcttaaaac cgccctgcac caaataatat tgttccagtg
    10741 gattcttcag cagcggcatt ttatagctga agtcgagggt ctgttccggc gcggaaatac
    10801 tggtactggt ggtcaaactg tgaccgtatg agttcatcca cggctttttc cacgtcgctt
    10861 tcacgcgcgg tcccacgtcc gtagagtaac cgaccccggt ttcgatggtg ttttctgttc
    10921 gcggcgaaac cacgcccgtc aatggtaata ctttcgtttc gcgcgcttta tcaaattgtg
    10981 gagccaccac caccgagtta aaccagccgg tagcagaaag tcggcggttc agttccgcca
    11041 gatctttcga ttcatactca tcgccctctt taaacggcac cagattttgc aggtattcat
    11101 cgcggatttg tgatccttca aaggtcacat gcccaaagcg gtaacgttcg ccactgttat
    11161 aatcaatatc ccagaaggct ttatgcaggc cgagcgcaat gcccagctgc gctttggtaa
    11221 attcgctatc gaaataacct ttacgcaacg caatactggt taaggacttt ttgaaatttt
    11281 cataatcgcc ctggttcagt accgtaccaa tagccgggcg agtatcgagc aatttcaaat
    11341 agtctttatc ggtccgcgcg ccgccgcgca ataccacatc ggtgccgcca attaacaccg
    11401 gcacacctgg cgtgactttg gcgatcaata cctgccgccc tttctttggc ggtggacgga
    11461 gatcaaattc aatggtcggc tggtaataac ccagcgcttt cagaccttca cggatggcat
    11521 catcgacgcg tgcgcgaaag cgacggtctg gcgtcacttc atcactttca atcgtagaaa
    11581 gctgcgcacg aacgttcttt tccagctgtc ccgataaccc ctcgacctgt agacggacgt
    11641 tcgcggcgac ggcagatccg cttaagcaga gtaagcttac acagcataac tgtcggatat
    11701 agcgcacatt ttctcctgaa tatccttttt tcctgcccct ggaacgccgt taaacggctt
    11761 aacaaaaatc cagtaatatg gattaaaaag cagactaaac cccaaatatt tcttatgttt
    11821 tactttagac ctattcacgg tggttattgt gtgcaaatac gcctcttgtt acaaccttga
    11881 ccccaatgac cgattttcgg gagagcgaca ccatgagttt atttgataaa aagcatctgg
    11941 tttcccccgc cgatgccctg cctggacgta acaccccgat gcccgtagcc acgctgcatg
    12001 cggtcaacgg tcactcaatg accaatgtac ctgacggaat ggagattgcc atttttgcga
    12061 tgggttgttt ctggggtgtg gagcgtctgt tctggcagtt acccggcgtt tacagcaccg
    12121 ccgcaggcta taccggcggc tatacgccaa atccgactta tcgggaagtg tgctccggtg
    12181 atacgggtca tgccgaagcg gtacgcattg tttacgatcc ttccgtcatc agctatgagc
    12241 agttgctaca ggtattttgg gagaatcacg atcccgccca gggaatgcgt cagggcaatg
    12301 accacggcac gcagtatcgt tcagcgattt atccgctgac cccagaacag gatgccgcag
    12361 ctcgcgccag tctggaacgt tttcaggcgg cgatgcttgc cgccgatgac gatcgtcaca
    12421 tcaccacgga aatcgctaac gccacaccgt tttattatgc cgaagatgac caccagcaat
    12481 atctgcataa aaacccatat ggttactgtg gaattggcgg aattggcgtc tgtctgccgc
    12541 cggaagcata gcgttacggg tacaaatgta gtttgttgat aaagtgctct ttgtctatgc
    12601 ctgatgcggc ttaaacgact taccaggcct acaaaatcgc ccaaattcaa tatattgcaa
    12661 ggactgcgta ggcctgatcg gcatagcgca tcaggcagta ttgcatttat cagcagtctg
    12721 aattttaacc ctctggcgac tttacagtac cttacgctat actagccact gaaaatgccg
    12781 gttcactttc ttcgaatcgg ctttcaatgt gtatttcaca caaattaatc aactttccct
    12841 tccgagggtc tggcctgaaa ggtcggataa gatatgttaa acagtatttt agtcatactc
    12901 tgcttgatcg ctgtaagtgc gttcttctcg atgtccgaga tctcgcttgc cgcctcacgc
    12961 aaaatcaaac ttaaactgct ggctgatgaa ggcaatataa atgcccaacg cgttctgaat
    13021 atgcaggaaa atcccggcat gttctttacc gtggtccaaa tcggtctgaa cgcagtggcg
    13081 attctcggcg gtatcgtcgg tgatgcggca ttttctccag cttttcacag cctgttctcc
    13141 cgctatatgt cggcagagct ctctgagcaa ctgagcttta ttctctcttt ctcgttagtg
    13201 actggcatgt ttatcctgtt tgcggattta accccgaaac gcatcggtat gattgcgcca
    13261 gaagcggtgg ctttgcgtat catcaacccg atgcgcttct gcctgtacgt ttgcaccccg
    13321 ctggtgtggt tcttcaacgg cctggcgaac atgatcttcc gtattttcaa actgccaatg
    13381 gtacgtaaag atgacatcac ttctgatgac atctacgcgg tagtggaagc cggtgcgctg
    13441 gcgggcgtgt tacgtaaaca ggaacacgag ctgattgaaa acgtctttga gctggaatcc
    13501 cgtaccgttc cgtcttcaat gacaccgcgt gaaaacgtga tttggtttga tctccacgaa
    13561 gatgagcaaa gcctgaagaa taaggtggcg gaacatccgc actctaagtt cctcgtctgt
    13621 aatgaagata ttgaccacat catcggttat gtcgattcta aagacctgct gaaccgcgtg
    13681 ctggctaacc aaagcctggc actgaacagc ggcgtacaaa ttcgcaacac gctgattgtg
    13741 ccggatacgt taaccctttc agaggcgttg gaaagtttta aaaccgcagg tgaagacttc
    13801 gcggtgatca tgaacgagta cgcgctggtg gtggggatca tcaccctcaa cgacgtgatg
    13861 accacgctga tgggcgatct ggtcggtcag gggctggaag agcagattgt cgcccgtgat
    13921 gagaactcat ggctgattga cggcggcacc ccaattgacg acgtcatgcg cgtgctggat
    13981 attgacgagt tcccgcagtc gggcaactac gaaaccatcg gtggctttat gatgtttatg
    14041 ctgcgtaaga tcccgaaacg caccgattcg gtgaaattcg ccggctacaa atttgaagtg
    14101 gtggatatcg ataactaccg catcgaccag ctgctggtga cccggatcga cagcaaggcc
    14161 accgcccttt cgccaaaact gcctgacgct aaagataaag aagaaagcgt cgcgtaaccc
    14221 ccagaaacat caacggctcc tgaatcagga gccgtcttat tactgcatag cactttggtt
    14281 aagccatctc tgtttgcaga cgcataacct gacggttaac ttcggacatc actgacaaat
    14341 gcagcttatc cttcactttt gggataagaa tcttaccctt atcaaattca aaagcgccaa
    14401 tgtccttgat gtataaccgt ccacggaaca ggatctttac gtacttcgcc acctgaagtg
    14461 ggttgtaccg ttggaaaatt ttcattgtta tctcctgctg agtattacgc ctgtgcggtg
    14521 ccacaatcgg cccaactatt atgaggcgca aattttaatg cttagtgact atagactatc
    14581 cgggcaatgt ttccaccgcg tataactttt tttacctaaa ggttacaatt attcagaatt
    14641 atctttttac cgaagcgcgg tcttcagtat aagcattcat ttttcatatg atttgtgcgc
    14701 ttgaccgcaa actggcatca cacttgcggg aaattcgata aatagcacat atgattaaaa
    14761 ctcagaccca agtggtcgga tcacctgcat atcataagaa ggaaacacca tgaccctacg
    14821 caagattctg gcactcacct gcctgctgtt gccgatgatg gcttccgcac atcagttcga
    14881 aatcggtcag cgagtgccgc cgattggcat taccgatcgg ggcgagttgg tgcttgataa
    14941 agatcagttt agctacaaaa cctggaacag cgcgcagtta gtgggaaaag tgcgagtact
    15001 gcaacatatt gctggtcgca cctctgcaaa agagaaaaac gcgacgctga ttgaagcgat
    15061 taaatcagcg aagttaccgc acgatcgtta ccagaccacc accattgtta acaccgacga
    15121 cgcgattccg ggttccggca tgtttgtgcg cagcagtctg gagagtaata aaaagcttta
    15181 tccctggtcg cagtttattg tcgatagcaa tggcgtcgcg cgcggtgcct ggcagctgga
    15241 tgaagaaagt tccgctgtag tggtgctgga taaagacggt cgcgtgcaat gggccaaaga
    15301 cggggcgctc acaccggaag aggtgcagca agtgatggac ctgctgcaaa agttgctcaa
    15361 ataatggcaa taacataaat tctggaaaaa taattttccc ctatcgttat ctatgggata
    15421 ggggaattaa gtctctgagt tcttacaccg aactaatact ggtaacgcta gttgctcttt
    15481 tttaaacgcc tcaatggtcg atttatattt ctcgcaaatc ttttctttgg taatattact
    15541 atctgcggcc agattcgcac ttactatcgc atccacttgt tcggctaatt cgacttgttg
    15601 gcaacctgca ttaaccagaa aattataaat aagagagtaa tcttcatcct ccaaaacaac
    15661 agaggaacca ttacgaaaac gttcatcccc tccatagatt ttaagcgcaa agcccaaaga
    15721 ttctatgact atggcaaaat tatcatcaat tattttttcg taaatatacc atccatcgca
    15781 ttctttaatt ccaccgcctg gtatttggag ttcaaattta ccccgtttta atttgcttac
    15841 tacatcctcc aataaggcat aaccgaaaga tacgtcacca aacgcctggc gaatataacc
    15901 tttaatcctt tttggtatgt cagactgttt tttctgcaaa atatcacagg catacaatct
    15961 tctgttttgt tctaacggaa actcgcggtt tttgaaatcc atgaatgagt tatactcatt
    16021 tattgttatg ggaatttctt cgtattcaaa cccactgcct cgcaatgtta ttgtatattc
    16081 agcaccgtta tctttaaggc tgaaactgat aagaaaatta tccatggcat taacagtttc
    16141 attttttgat gaaagcactt gataactatt tttatcaatc tgtgttgcgc aacgaaagga
    16201 ctcccgcaaa gttaacagac tatcataatt cgaaacccaa tcttttttct taaatgcatg
    16261 ctgaatatta ttaaaaaaat cgtttgaatg tgtttttgta tgcgatgact ttacaaataa
    16321 ctccaccgca ccaataaaat tattgtgtct gatcctgggt aacatatggc taccttattt
    16381 tatttggtta ttttatgtca ccattaaata tctcattata gaagtaaact atcaaaaaac
    16441 gctcattatt aaaatgtaac ttactagcaa ttgaattttt gcactttaaa tacaatctct
    16501 ttttatttat aactttttag gttgggataa atcaggatgg cagaatcagg aaatacactg
    16561 tttctgccat ctgaatttag taaatggaca ctctgaaccc cggattcagg aacgactcgc
    16621 gcggagtgta atccagcggt ttaccctgcc agtcgtgaac gtgcgctccg gcagctgcag
    16681 ccacagcatg tccggcggcg gtgtcccaaa tattcgttgg tccgaagcgc gggtacagct
    16741 gcgcctgtcc ttccgccacc aggcagaatt tcagcgaaga gccgatggac gtggtctgat
    16801 gttcgccaag ctgttgcaga tactctttca gctccgcatc tgcatgggaa cggctgataa
    16861 ccaccagcgg cgggcgcgca tcgcggacct gaatcagctt gcgcacaccg cactcttctt
    16921 tccaggcctt gccttctgcc gcgctgtaca ttacgttcat taccggcgca tacaccacac
    16981 ctaaaatcgg tttgccatgg tcaatgagcg caatgttaac ggtgaattcg ccattacgtt
    17041 taataaactc tttagtacca tccagcgggt ctaccagcca gtaacgctgc cagtgctgac
    17101 ggacttccca accgggagga tcttcttcag aaaggaccgg aatatccggt gccagcgtac
    17161 gtaaaccgtc catgataacg gtgtgagcgg caatatccgc tgccgttacc ggagaattgt
    17221 ccgctttgct gacgacgtcc atcggtttcg tcccgtcgta gacctgcata atggcatcgc
    17281 ctgcattccg tgcaagctgg catacttgat ctaacatttc tccacctcgt ctctgtgagc
    17341 ggtgttaact tattgtttta cttataccct atcgttaatg aatgcgccaa ctgtgatagt
    17401 gtcatcattt tcaaagcgta aaattgtggc attcttcact gttctataag taaggcgttt
    17461 attctccctt ttctttcgta ttcccgatga taaaaggatg tccctgatga ttaagtttag
    17521 cgcaacgctc ctggccacgc tgattgccgc cagtgtgaat gcagcgacgg tcgatctgcg
    17581 tatcatggaa accactgatc tgcatagcaa catgatggat ttcgattatt acaaagacac
    17641 cgccacggaa aaattcggac tggtacgtac ggcaagcctg attaacgatg cccgcaatga
    17701 agtgaaaaac agcgtactgg tcgataacgg cgatttgatt caggggagtc cgctggccga
    17761 ttacatgtcg gcgaaaggat taaaagcagg tgatgttcat ccggtttata aggcgctgaa
    17821 tacgctggat tatacggtcg gtacactcgg caatcatgaa tttaactacg gtctggatta
    17881 cctgaaaaat gcgctggcgg gagcgaaatt cccttatgta aatgccaacg tcattgacgc
    17941 cagaaccaaa cagccaatgt ttacaccgta tttaattaaa gataccgaag tggtcgataa
    18001 agacggaaaa aaacagacgc tgaagattgg ctatattggc gtcgtgccgc cgcaaatcat
    18061 gggctgggat aaagctaatt tatccggaaa agtgacggtg aatgatatta ccgaaaccgt
    18121 gcgcaaatac gtgcctgaaa tgcgcgagaa aggtgccgat gtcattgtcg ttctggcgca
    18181 ttccgggctg tctgccgatc cgtataaagt aatggcggaa aactcagttt attacctcag
    18241 tgaaattccg ggcgttaacg ccattatgtt tggccatgct cacgccgttt tcccgggtaa
    18301 agattttgct gatatcgaag gggctgatat caccaaaggc acgctgaatg gtgttccggc
    18361 ggtaatgccg ggcatgtggg gcgatcatct tggggtggtc gacttacaac tcagtaatga
    18421 cagcggtaaa tggcaggtga cgcaggcgaa agcggaagca cggccgattt acgacatcgc
    18481 caataaaaaa tccctcgcgg cggaagacag caagctggta gaaacactca aagccgatca
    18541 cgatgccaca cgccagttcg tcagcaagcc aatcggtaaa tctgccgaca atatgtatag
    18601 ctatctggcg ctggtgcagg acgatccgac cgtgcaagtc gtgaacaacg cgcaaaaagc
    18661 gtatgtcgaa cattacattc agggcgatcc ggatctggca aaactgccgg tgctttcagc
    18721 tgccgcaccg tttaaagttg gtggtcgcaa aaatgacccg gcaagctatg tggaggtgga
    18781 aaaaggccag ttgaccttcc gtaatgctgc cgatctttat ctctacccca atacgctgat
    18841 tgtagtgaaa gccagcggta aagaggtgaa agagtggctg gagtgctccg ccggacagtt
    18901 taaccagatt gctcctaaca gcacgaaacc acagtcactc atcaactggg atggtttccg
    18961 cacttataac tttgatgtta ttgatggtgt gaattatcag attgatgtta cccaacccgc
    19021 ccgctatgac ggcgagtgcc agatgattaa tgccaatgca gagcggatta agaacctgac
    19081 ctttaatggc aagccgattg atccgaacgc tatgttcctg gttgccacca ataactatcg
    19141 cgcttacggc ggcaaatttg ccggtacggg cgacagccat atcgcttttg cttcaccgga
    19201 tgagaaccgc tcggtgctgg cagcgtggat tgctgatgag tcgaaacgtg cgggggaaat
    19261 tcatccggcg gcagataaca actggcgttt agcaccgata gctgccgata agaaactgga
    19321 tatccgtttc gaaacttccc cgtcagataa agccgcagcg tttattaaag agaaagggca
    19381 gtatccgatg aataaagtcg cgaccgatga tatcgggttt gcgatttatc aggtggattt
    19441 gagtaagtaa aacacttctt tttcggccta taaatcaacc gcatccggca tttattggcg
    19501 gatgcgatgc tgccgtatct tatccaccct acaagccatg aaccgtagac cggataagcg
    19561 cagcgcatcc ggcagttatg ccgcacgttc atcccgcacc gccaacacct cgggcaaatt
    19621 caactcaatc cagtccgcca gtgcagcaac cttttcgctc acctgctcgc ccagcggcgt
    19681 gaggctatat tccacatgcg gcggcaccac cggatacgcg atacggttaa gaaaaccatc
    19741 ctgttctaac gcctgtaacg actgcgcaag catcttttca ctcaccccac cgattttgcg
    19801 ccgcaggtcg ctaaagcgat gagtaccttc gcgtagcgcc accagaatca acacccccca
    19861 acggctggtg acgtgtttca acacctcgcg cgacgggcac tgttccgcaa agagattacc
    19921 ctctttcagt tgttgtgaca ggctaacctg actcatttca tacttacctt tttgcacgta
    19981 cttactaaaa gtaagtttag gtgttagcgt atttaaacac aagacaaaac gatggagact
    20041 tcccatgatc gctattactg gtgccactgg ccaacttggt cactatatta ttgaatcctt
    20101 gatgaaaacg gttcctgcca gccaaatagt ggctatcgtt cgtaatccgg caaaagccca
    20161 ggccctggca gcacaaggca ttaccgtgcg tcaggctgac tacagcgatg aagccgcact
    20221 gacatctgca cttcagggag tggaaaaact actgctgatc tcttccagcg aagtgggtca
    20281 acgtgccccg cagcatcgta atgttattaa tgccgcaaag gcggctggcg tgaaatttat
    20341 cgcttatacc agcctgctac atgcagatac ctccccgctc ggcctcgccg atgagcacat
    20401 cgagacggag aaaatgctgg ctgattctgg catcgtttac accctgctgc gcaacggctg
    20461 gtacagcgaa aactacctcg ccagcgcccc ggcagcactg gaacacggcg tatttatcgg
    20521 tgcggcgggc gatggcaaaa tcgcctcagc aacgcgggca gattatgcga cagctgcggc
    20581 acgcgtgatt agcgaagccg gtcacgaagg caaggtttac gaactggcgg gcgatagtgc
    20641 ctggacgttg acacagttag cggcagagct gaccaaacag agcggcaaac aggttaccta
    20701 tcaaaatctg agcgaagccg atttcgccgc ggcactgaaa agcgtcggac tgcccgacgg
    20761 actggcggat atgctggcgg attctgacgt tggcgcatcg aaaggtggtc tgtttgataa
    20821 cagcaaaacg cttagcaaat tgattggctg cccaacgaca acgttagccg aaagcgtaag
    20881 ccatcttttt aatgttaata actagttaat taaagtggca tcctcccgca tcctctctga
    20941 taatgacggg atgccgggag caatcatgtc tgcttcctga acgtttcttc tgacagacca
    21001 atggatgcca gtaatgatta gcggcgtgct gtacgccctg ttagcagggt tgatgtgggg
    21061 gcttattttt gtcgggccgt tgatcgtgcc ggaatacccg gcgatgttgc agtcgatggg
    21121 gcgttatctg gcgttagggt taattgcgct acccattgcc tggctgggac gcgtgcgtct
    21181 gcgtcagttg gcgcgtcggg actggcttac cgccttgatg ctcactatga tgggcaacct
    21241 catttattac ttctgccttg ccagtgccat tcaacgtact ggcgcgcctg tttccacgat
    21301 gattatcggc accctgccgg tggtgattcc tgtctttgcc aatctgcttt atagccagcg
    21361 cgacggcaaa ctcgcgtggg gaaaactcgc cccggcactg atttgtattg gcatcggcct
    21421 ggcgagtgtg aatattgctg agttaaacca cggactcccc gattttgact gggcacgtta
    21481 tacctctggc atcgtgctag cgttagtttc cgtggtctgc tgggcatggt atgccctgcg
    21541 caacgcccgc tggctgcggg aaaatcccga caaacatccg atgatgtggg cgacggtgca
    21601 ggcgctggtc acgctgccgg tttctctcat cggctatctc gtcgcctgtt actggctgaa
    21661 tatacaaacg ccggacttct ccttaccttt tggcccccgt ccgctggtgt ttattagtct
    21721 gatggttgcg atagccgtgc tttgctcatg ggttggcgca ctctgctgga acgtcgccag
    21781 ccagcgatta ccgacagtga ttctcgggcc gctgattgtt ttcgaaacgc tggcaggttt
    21841 gctgtacacc tttttactcc gccagcaaat gccgccgcta atgacgctga gcggtatcgc
    21901 gctgttagtg attggcgtgg tcatcgcggt cagagcaaaa ccagaaaaac ctttaactga
    21961 atctgtctca gaaagttgac atgctggcag tgagttaaat aagcctctgc tacgtaaggg
    22021 ttatagcttt tgccttaaag gtgtatttaa aatacatctt atcttattaa gaatgaggta
    22081 tcagctatgg cttatcgcga ccaaccttta ggtgaactgg cgctctctat ccctcgcgct
    22141 tcggctctgt ttcgtaaata tgatatggat tactgctgtg gcggtaagca gacgctggcg
    22201 cgcgcggcgg cacgtaaaga actggatgtt gaggtcattg aagctgaact ggcaaagctt
    22261 gctgaacaac cgattgagaa agactggcgt agcgccccgc tggcagaaat cattgaccat
    22321 atcatcgtgc gctaccacga tcgtcaccgc gagcagctgc cggagctgat cctgcaagcg
    22381 actaaagtcg agcgcgttca cgccgacaaa ccgagcgtgc caaaagggct gacaaaatac
    22441 ctgaccatgc tgcatgaaga gctttccagc cacatgatga aagaagagca gatcctcttc
    22501 ccgatgatca aacaaggcat gggcagccag gcaatggggc caatcagcgt aatggaaagc
    22561 gagcacgatg aagcgggcga actgctggaa gtgatcaaac acactaccaa taacgtcaca
    22621 ccgccgccag aagcctgcac cacctggaaa gcgatgtata acggcattaa tgaactgatt
    22681 gatgacctga tggatcacat cagtctggaa aacaatgtac tgttcccacg cgccctagcg
    22741 ggtgagtgat ctaaaagctg gatggcattg cgccatccag catgataatg cggttatttc
    22801 cgcagttcag cagcccgctt cttaccaata aacagccagc ccaaccccag cgcgataaac
    22861 cacagcgggg taaccagcag cgcctggcga gtgtcatctt ccagtgtcag caacacgacc
    22921 acgaacacaa agaacgccat acatacccag cacatcagct tgccgagcgg catcttgtag
    22981 atcgactttt catgcagatg aggacgctgt ttgcggtaca ccaggtacga gcaaaggata
    23041 atcgtccaga cgaacataaa cagaatcgcg gaaacggttg taatcatcgt gaacgcgcca
    23101 atcacgctag gattcacata caacatcacc acgccgccga gcagacagat acacgagaag
    23161 gtcaaccctt tcgcgggtac tgcgcgctta gaaagtttag cgaacgcttt cggcgcaaca
    23221 ccttcctgcg ccagaccaaa cagcatacgg ctggtagaga agacaccgct gttagcggaa
    23281 gacgccgcag aggtcagcac cacaaagttg atcacgctgg cagccgcagg caaacctacc
    23341 aacacaaaca gctcaacgaa cgggcttttc tccgggacta ccgaactcca cggtgtcacg
    23401 gacataatca caatcagcgc gaagacgtag aacataatga tacggatcgg aatggagtta
    23461 atcgcgcgtg gcagtgattt ctccggatct ttggtttccg cagctgttgt acctaccagc
    23521 tcaatcccca cgaaagcgaa aaccgctatc tggaatccgg caaagaagcc acttaaacct
    23581 ttcgggaacc agccgccgtc attccacaaa tgcgcgaatg acgcttccac accggtcggt
    23641 gactgaaagt gcatcgccac catgaccagg ccgacgacaa tcagcgacac gatggcgacg
    23701 attttgatca tcgcaaacca gaactccatc tcaccgaaca ttttcacggt ggcaaggttg
    23761 agcgtcagca gcagcactat caccgccagc gaggcgaccc agtcggagag atcggggaac
    23821 cagaactgag cataagccgt gatcgccacc acgtctgcca taccggttac aacccagcag
    23881 aaccagtaag tccagccggt gaaatatccc gcccacggcc ccagtaaatc ggaagcgaag
    23941 tcactaaaag atttgtattc cagattcgaa agcagcaatt cccccattgc ccgcatcacg
    24001 aaaaagagca taaaaccaat gatcatataa acgaaaatga tcgacggccc ggcaaggcta
    24061 atcgttttgc cggaccccat aaacaaccca gtaccaatgg cacctccaat ggcaataagc
    24121 tgaacatgtc ggtttgtgag attgcgccgt agcgactgtt cagccggagc ctgatcatcg
    24181 gcaacgactt ttacctgatc taccatgttt tttcttcctg tacctgtctg tgttgttcag
    24241 gctctacggc ctttagtcag tctatgatac gacgaatctg tatcgggact catcgatatt
    24301 aggtaacaat acgcgggatg aatactaaga tttagatcta atgttaattt tatgtttaaa
    24361 gtgagtgtca tatcacggtg ataacgcgaa acagctcaca aaaatacacg cttacggggt
    24421 ctttgcaaca caaaatagcg ccgtttggca aactgaaggg tttattgctg aatgcctgct
    24481 cccctctggt tcgaggggaa cagaatgcgc tgcttagagg atttccagca gttcgacttc
    24541 aaacaccagg gtgctgaacg gagggatgga tgcacctgcg ccgcgctcgc catatgccag
    24601 ttcctgcggg atagtcagtt cccatttaga acctaccggc atcagagtca gtgcttcaat
    24661 ccagccaggg atcacgccat taaccgggaa ttcagcgggt tcaccacgag caacggagct
    24721 gtcaaacacg gtgccgtcga tcagtttacc ggtgtaatga acacgaacgc ggtcggtacg
    24781 tgccggaatt gcgccttcac cctggttgat cacgcggaat tgcaggccag attcggtgct
    24841 attcacacct tcttttttgg cgttttcttc caggtatttc acgccttcag cagccatcgc
    24901 ctggaaacgc tgacgacgaa cggcatcggc gcgttcgtgg atttcacgca gcgcgcgatg
    24961 caccacatca accggcacag ccggatgttt gccttccagc gcatcggcaa tacctgcaac
    25021 cagtgcttct ggcagcagcc cttccaggcc agattcactc agttgttgcc ctacctgcaa
    25081 accaatgccg tagcttgctt gcgcttcgat ggtgtcaaaa gttggggtgg tcatcgtatt
    25141 tcctttctta gggtataaaa gtagcgggca gcatatcagc cacacttctt tgggtaaaac
    25201 tttgtcgcag gaatgatgac aaatcccggg gaaaaagaaa caatagcaat atcccgtatg
    25261 aatataataa aggcgggttt accacggaca tcatagcgtt agcttattca taggctatga
    25321 ttgaggaaca agacgtggag caggaggaaa ccatgcccgg gcgctttgaa ctaaaaccaa
    25381 ccctggagaa agtctggcac gcgccggaca attttcgctt catggacccg ctgccgccga
    25441 tgcatcgtcg ggggatcatt attgccgcca ttgtattggt ggtcggattt ctgcttccat
    25501 ctgatgatac gcccaacgcg tcggtggtga cgcgtgaagc gcagttagat attcagtcgc
    25561 aatcacaacc gccgacagaa gaacagccgc aaactaccca aacgcaacca ttccagccag
    25621 atagcggaat tgataatcaa tggcgctctt accgtgtcga acccggtaaa acgatggcgc
    25681 agctgttccg cgaccacgga ctgcccgcta ccgatgtcta tgcaatggcg caggtagaag
    25741 gcgcgggtaa gccgctgagt aatttgcaaa atgggcagat ggtgaaaatc cgccagaacg
    25801 ccagcggcgt ggtgacgggc ttaaccattg atacggggaa taatcaacag gtcctgttta
    25861 cccgccagcc ggatggcagt tttattcgtg cgcggtagat gaagggtcaa ccaaaagcca
    25921 gctaaggatt gtctctttga tgaccttacg tctggcttcc agtgcctggg gggaaaccat
    25981 actgatatga taaactaggc caagcgtatg ctggttgatc aaataatatc ccccaagcgc
    26041 agcaatatta atattcacct gcaatgggtc gatacctggc ttaaaaatat tgtatttctt
    26101 cccttcatcc aataaggatt ccattaactg caagtgagca tggttaattt ctaagagacg
    26161 ctgtgatttt gcataatgca cacctttact ttgattttca ctatgcacaa tttttaaaaa
    26221 ccaaggatta gcaatataat aatcccatgt aaaatcaata attttctcaa tggctttttg
    26281 tggcggaaga tctgagaaat caagggcctt ttctttttgt cgaatatcgt tccaggtata
    26341 ttccagcact tcgataaata aattttcttt attaccaaag tggtgataaa ccgtctgttt
    26401 actacaccct gctgccttta caatattatc tactctggcc ccttcataac catattcggc
    26461 aaattcatta accgcacata gcagcaattt ttccttcagg ctctgtacgt tgctggtatc
    26521 aatatacata acgcttcccc acgacctctt cgtggtgact atcataacct gatttaattt
    26581 cagcttcaga aaaaagggca aaaacttgta ctttcatcag gttatcaaat gtcctgtgat
    26641 cgccatcgca tatcaaccaa ctagatggtt gatcttttta tcctccagtc gcaagctaat
    26701 catcaccaag gaaacaaaat tcaaaaaaga gagctattgt atgaaatcca ctcgtcctgt
    26761 tgccgtgatt accggagcca ctcgcggtat tggaaagggc tgtgcgcagg agcttgctcg
    26821 tggcgggttt aacctgctga ttaacgatcg tcccgatgcc gacagcgtag aaaaactgca
    26881 tatcacacaa caagaatgtt tggcggaagg cgtggaagtg atttgcttcc ctgctgatgt
    26941 cggtgatcta tctctgcatg aggagatgct cgacgcggcg caaaaccagt gggggcggct
    27001 ggactgtctg ctcaataacg cggggatttc agtgaaaaaa cgcggtgacc tcctcgacct
    27061 cgaaccagac agcttcgatc aaaacattgc cattaacacc cgcgcgccgt tcttccttgc
    27121 tcaggcattc agtaagcgct tactggcaca gccgaaaccg gaagcggagc tgccgcaccg
    27181 ctcgattatt ttcgtcagtt ccatcaacgc catcatgctg gcgatgaatc gcggcgaata
    27241 caccattgcc aaaaccgccg tttctgccgc tgctcgtctg tttgctgctc gcctttgtaa
    27301 tgaacaaatt ggcgtttatg aagtgcgccc tggtctcatc aaaactgata tgactattcc
    27361 ggcaacggct tattacgacg aactgattgc caaaggtctg gtgccgtggg gacgttgggg
    27421 ttatccggct gatattgctt ccaccgtccg cgcgatggcg gaaggtaaac tgatttacac
    27481 ctgtggtcag gcggtcgcaa tcgacggcgg cttaagtatg ccgcgcttct gaggctgaat
    27541 catgagagat ctgttacaac gcccggattt attcagtatc aataccgcga cactgggcta
    27601 taaaacgccg ctacccgcca ttattgatgc ctgcgccgca cgcggtattg gcgcaattgc
    27661 cccctggcgc agggagttgc agggtgaaga tctccagcaa atcacccgcc agcttgccgc
    27721 cagtaacatg agcgtttccg gtttgtgccg tagcacttac tacaccgcgc cgacactggc
    27781 ggaacgcaaa ctggcgattg atgataaccg ccgggcgctg gacgacgcag cggtgcttaa
    27841 cgccgcctgt tatatgcagg tggttggcgg tctgccaacg ggcactaaag atctatatga
    27901 agcgcgagaa caagtgaaac aagggattcg ccagttgctg ccacattcga aagatgtcgg
    27961 cgtgccaatt gcgctggagc cactgcaccc gatgacagct gccgatcgct cttgcctgtg
    28021 cacgttacgt caggcgctgg actggtgtga tgaactcgat cccgatggtg agtttggcct
    28081 cggcgtggcg gtggatgttt atcacgtctg gtgggatccg gatctcgcca gtcagatcct
    28141 gcgtgccgga aaacgcatcc tcgcgtttca tgtttccgac tggttaatgc cgaccactga
    28201 tctggtcaat gaccgtggta tgccgggaga tggcgtcatc aatattccgt caattcgtcg
    28261 gctggttgaa aacgccggat ttaatggtgc tattgaactg gaaatattct cgccttactg
    28321 gtggcagaaa gatattaaca g
//
LOCUS       DAJTQY010000052        27582 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.052, whole genome shotgun sequence.
ACCESSION   DAJTQY010000052 DAJTQY010000000
VERSION     DAJTQY010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27582
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.052"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            49..156
                     /locus_tag="OO349_002271"
     CDS             49..156
                     /locus_tag="OO349_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCR5867324.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(243..1922)
                     /gene="bcsG"
                     /locus_tag="OO349_002272"
     CDS             complement(243..1922)
                     /gene="bcsG"
                     /locus_tag="OO349_002272"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HCR5867325.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDIWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIDFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(1919..2110)
                     /gene="bcsF"
                     /locus_tag="OO349_002273"
     CDS             complement(1919..2110)
                     /gene="bcsF"
                     /locus_tag="OO349_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HCR5867326.1"
                     /translation="MMTISDIIEIIIVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(2107..3678)
                     /gene="bcsE"
                     /locus_tag="OO349_002274"
     CDS             complement(2107..3678)
                     /gene="bcsE"
                     /locus_tag="OO349_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HCR5867327.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            3951..4139
                     /locus_tag="OO349_002275"
     CDS             3951..4139
                     /locus_tag="OO349_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjR family protein"
                     /protein_id="HCR5867328.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            4151..4903
                     /gene="bcsQ"
                     /locus_tag="OO349_002276"
     CDS             4151..4903
                     /gene="bcsQ"
                     /locus_tag="OO349_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HCR5867329.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLCYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCEHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            4900..5259
                     /locus_tag="OO349_002277"
     CDS             4900..5259
                     /locus_tag="OO349_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
                     /protein_id="HCR5867330.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYTHINASRPRPLDPVRYLIQTCWLLIGASRKETPKPR
                     SGHFQVCKIFVDVTING"
     gene            5256..7517
                     /gene="bcsA"
                     /locus_tag="OO349_002278"
                     /pseudo
     CDS             5256..7517
                     /gene="bcsA"
                     /locus_tag="OO349_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077779.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
     gene            7528..9867
                     /gene="bcsB"
                     /locus_tag="OO349_002279"
     CDS             7528..9867
                     /gene="bcsB"
                     /locus_tag="OO349_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HCR5867331.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMSGEQGADAPIVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNFEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLATISVILLAWVLWRLLRIISSRRLNPDNE"
     gene            9874..10980
                     /gene="bcsZ"
                     /locus_tag="OO349_002280"
     CDS             9874..10980
                     /gene="bcsZ"
                     /locus_tag="OO349_002280"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HCR5867332.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYMPPTLAQYFTRFGAPWTTLRETNQHLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDTYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            11039..11215
                     /locus_tag="OO349_002281"
                     /pseudo
     CDS             11039..11215
                     /locus_tag="OO349_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774071.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
     gene            11543..13291
                     /gene="bcsC"
                     /locus_tag="OO349_002282"
                     /pseudo
     CDS             11543..13291
                     /gene="bcsC"
                     /locus_tag="OO349_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005138097.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsC"
     gene            13373..15361
                     /gene="hmsP"
                     /locus_tag="OO349_002283"
     CDS             13373..15361
                     /gene="hmsP"
                     /locus_tag="OO349_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HCR5867333.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNFSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            15546..16832
                     /gene="dctA"
                     /locus_tag="OO349_002284"
     CDS             15546..16832
                     /gene="dctA"
                     /locus_tag="OO349_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HCR5867334.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            17053..18549
                     /locus_tag="OO349_002285"
     CDS             17053..18549
                     /locus_tag="OO349_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR5867335.1"
                     /translation="MQGIKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASDSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(18645..19574)
                     /gene="kdgK"
                     /locus_tag="OO349_002286"
     CDS             complement(18645..19574)
                     /gene="kdgK"
                     /locus_tag="OO349_002286"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HCR5867336.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLASEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRAKG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEALVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            19806..20573
                     /gene="pdeH"
                     /locus_tag="OO349_002287"
     CDS             19806..20573
                     /gene="pdeH"
                     /locus_tag="OO349_002287"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HCR5867337.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHICLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPMETLNTAVLAL"
     gene            20643..22703
                     /locus_tag="OO349_002288"
     CDS             20643..22703
                     /locus_tag="OO349_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCR5867338.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGNLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYIFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(22885..24207)
                     /locus_tag="OO349_002289"
     CDS             complement(22885..24207)
                     /locus_tag="OO349_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HCR5867339.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVSSI
                     LGASVAPYIAAWLQANYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(24618..25631)
                     /gene="yhjD"
                     /locus_tag="OO349_002290"
     CDS             complement(24618..25631)
                     /gene="yhjD"
                     /locus_tag="OO349_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HCR5867340.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(25680..26579)
                     /locus_tag="OO349_002291"
     CDS             complement(25680..26579)
                     /locus_tag="OO349_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5867341.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPSGI
                     AKNLLLPRLSEFLYQHPGIELELSSHNRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            27099..>27582
                     /locus_tag="OO349_002292"
     CDS             27099..>27582
                     /locus_tag="OO349_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR5867342.1"
                     /translation="MQIVMFDRQSIFIHGIKISLQQRIPGVSIQGVSQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     E"
BASE COUNT         6567 a         6955 c         7561 g         6499 t
ORIGIN      
        1 aactcaagaa tagccgcgaa acgttggcat tacaacacag gcggctatat gacgctcgca
       61 gaactgggca tggccttctg gcatgattta gcggctccgg tcattgctgg cattcttgcc
      121 agtatgatcg tgaactggct gaataagcgg aagtaacgtg tcatgcgggc gtcaggctgc
      181 cgtaatggca atttgcgccc ggatcaggcc gcagaggtta aactctgcgg cctttttcgt
      241 gcttactgcg ggtaaggcac ccagtcgccg ccgttcaggc gaacgtacgg tttatcttgg
      301 tattgaataa ctactgcatt tgagttctcg gagaccggtg ctgtttgtgg caacccactg
      361 gtgagttttt tccagtcaac attgtcttcg gtgaaaatct tgccatcaag aacgcgaacc
      421 accagatcgg agatagccag gaagctgctc ggttggtcaa tgacaatcgg tgccccctga
      481 tgtggtgcct tcatgccgaa gaatttcacc ccaacgggga cgtctgtgat agacgggcta
      541 gggatatcac gtaggccaga tacctgcatt ctgtcgccct tcagcgcgcc gccgtgttcc
      601 ggcaccacga ccaccatcac tttacgaccc gatttctcca gttcagtaaa gaaggcgtcc
      661 agttcatcaa agaatttctg cgcccgcgct ttgtaatccg ctgttttgct gacccccgga
      721 taatggttgc cgtcatgcag tggaagcgtg ttgtagaacg tggcactacg gctgttttta
      781 tctttttcgg taacgtccag ccagcggtta agcacggcgg tatcgtcata aaccggcgaa
      841 ccatcaaagc ccagcaaaat aaccggcaga tttgtttgat ccatcaattc agtctgcatg
      901 ccgccatttt cgcgaacttc tttcaaaaaa ccgccgaact ggccgttatg tcccatcatc
      961 aggtgctggg taaagcccag tttcgaaagg ttatcaaaca gatagcagtc gttatttgcc
     1021 ggttgataca gattagtgtg cgaagtctgc ccgcagctgg cgcgcagtaa acggatcgcc
     1081 gccgggccac tgtaggaggt ggcggagtta aagttcttga aatcaatatc gaaatgcgac
     1141 cacagtggat gcgacatcaa cccggcggct tctatatccg accaggaaag cgaacagatg
     1201 ttaatcacca gtagttcaaa tggctgagca tcagcgggca gcgaagacgg gaaggtcgat
     1261 ttacgtttcg cctccgcgtt atagaaatta ttcagccagg cgttaaggtt cgccgttgtt
     1321 ggcggtgcag tttgtgcggg catatcaccc actaccggtg cgccacccgt cgccgcaacg
     1381 gttgccgctg cgttaccacc cgtcgttgtt acagtggtcg tcggttgtcc ggctggccac
     1441 aaggagaaac ttggtcccgc caaggtaagt acgttcagcc atagcagtat ggcaaccaca
     1501 aaaacggtaa tgcgaatcca ttgtgacagg aataaccagg ccactaataa aacaaaaatg
     1561 gccccaatca tctgccagtt aataaagcgt gtgacaaggt cgattaaata atcggtactg
     1621 aaccccgcca cctgcgaacc ctggctcatt atgctttccg ggccaggcaa ccagatgtca
     1681 tgccagaaca aagcaaagcc gatcggcagg gcaatccagt ggcgcaagcg atgcaggctg
     1741 taacgcggaa tgggcatcag cagaaacgcg gcaaacacca aattgaggag cgggtggaag
     1801 ttaagatatc ccgcccacaa caggccaaac ttaaccagaa aatagaagtt ccagccggaa
     1861 aggccgcgcc agtattgcca gagggaagaa ggcatggcgg tattttgcgt aaattgagtc
     1921 attttttggt tgccctggct ttttccgtgc ggcgtaacgt cccggccggt ttaacataac
     1981 gaggtttagc aaagaacaaa cgtaaggtgt cgcgaatgcg tcgcaaagag tgccgcgcca
     2041 gatagcccag cgggaaaaat atcagtgcgc aaacgataat aatttcaatg atatcgctga
     2101 tggtcatcat gatgagcgct ccacagcatc atctaacagt cgcatgggtt ctggtattcg
     2161 tcgccagtgg cgaccatcgt gctcggcatt gatgaccggt ttagaacttt gcgttaaagg
     2221 cagcggcatt ccccattgtt ctggggcaag caggcgcatc tgcaccagct cggcactgat
     2281 ttgatcatct tcaaaccaga ccatacggtt tgagaaaatg tcgccagtag gcaatgggaa
     2341 aatatgattc aacgcggtat ccagatcgtt aatccgacag aatgagagaa acagcaccag
     2401 ccgattaccg ccaatggtca tgatatcgcc ggtacggtta gggcgacata gcgtcagggc
     2461 ttgctcaacg cggatacccg gtaccggacg tagggcaacc agaacgcctt taccgtgggc
     2521 aggcaagaga gggttattca tcatgttgtt gacggcatta cagaacacat cccacttctg
     2581 gaaaccacgc agtttgagcg gctgggtcat tgacagcaag gtagtgatat cttccggtac
     2641 atagcgacta aacttctgcc cttgcacgct ttcgatcatc gtcagacaac gggagagtgg
     2701 cgcattccac ggaataacca tatttgcacc gcaggccaat aacaaacgtt catcggtggc
     2761 gcgcaggcta gcggtatttt cccgcacgag gattttcatc gcactaccgc gctggcgacg
     2821 cagggtatga atgctgcggg ccagtggctc gatttgcgca ttttgctgta aagaaaagac
     2881 caccgtcgcc gcctgagcgg tacgggcttc attgaacagg acttcgttat tgttgaacag
     2941 ttgccagtgt tccgatagcg gcggcgcacc ttccagtaca gcaacattac tcagaatgcg
     3001 tttttcgtcg ctgcgtggtt ggatctccgc ctcttcgctt tgaactaatg tccagatacc
     3061 attttgttgc tgaacgctaa gctgctgacg ggcgctgacc cctttttcgt tgcaccagaa
     3121 ggcaatatcc agcaaatgtt ggtcaccctg aaaacgcaaa ctggcaagac caaaaagtga
     3181 acggtattcc tcaagcaata atgaaaattg tttatcgtta ttatttccgg gattaattac
     3241 caaaagcgaa caatggttta acctgctcca tttattcatt ttatccaacc atgagcgaag
     3301 ccgctcggca ggaatatttt gccatgcgtt atttgcgcaa acaagaataa aaaggtaatt
     3361 atggggatca atagaacact gcaaatcacg ggccaaatag tatagacctt tttcatgatt
     3421 tagcattgaa aataatttta ttttttccgg cccttgagaa tcatctaatt gaaagatttt
     3481 cgccggatcg ctgtccatgc taatgaccgc gacgtgtgcg gtttcagcct gggatgcaat
     3541 tgtttgattc gccagactga tagcatcttc atggcgatcg acgttaaacc accagacgcc
     3601 gcctgctggc atatgtcgca gctcatccca taatgatgag ataccgatag agaatacagg
     3661 gtccacaatg tccctcatgt caccattttg tctgattatc agtttactag cgaaagctaa
     3721 gaaataaacc taccattgaa attaaaactt atcagattta gcatgtgaat caatttagaa
     3781 ggtcacaaga cgtgtgattt tagtcttttc gtttttaact ttttctgttg cgtaacggaa
     3841 agtcaaaaag tgagcaaatt cccgtctcgc cgtaaacaat aaaccggcga aatgctcacg
     3901 gttagaattg tctcatgaat ggtacggtta tttcataggg atcaagcaaa atgaataaca
     3961 atgaaccaga tactctgcct gatcccgcga taggctatat cttccagaat gatattgtgg
     4021 cgttaaagca ggcattttca ctgcctgata ttgattatgc cgatatttcc caacgcgaac
     4081 agttggccgc ggcattaaaa cgctggccgt tgctggcaga gtttgcgcaa caaaagtagg
     4141 ggattggtga atggccgtac tgggattgca gggggtgcgg ggaggcgtgg ggacaacaac
     4201 catcaccgcc gcattagcct ggtcattaca aatgttggga gaaaatgtcc tggtggtcga
     4261 tgcctgcccg gacaacttgt tgcgcctgtc atttaatgtt gattttaccc accgtcaggg
     4321 ctgggccaga gcgatgctgg atggccagga ctggcgtgac gctgggttgt gctacacctc
     4381 gcagctcgat ttgctgcctt ttggtcagtt atccattgaa gaacaagaaa atccacagca
     4441 ctggcaaacc cggctgagcg atatttgctc cggcttacag caactaaaag ccagcgggcg
     4501 ttaccagtgg attttaatcg acttaccgcg tgatgcctcg cagataaccc accagctgct
     4561 gagtttgtgc gagcactcgc tggcaatcgt caatgtggat gccaactgcc atatccgact
     4621 gcatcagcaa gcgctgccgg atggcgcaca tattttgatt aatgacttcc gtattggcag
     4681 tcaggttcag gacgatattt accagctttg gttgcaaagc cagcgccgat tactgccgat
     4741 gctcattcat cgtgatgaag cgatggctga atgcctggcg gctaagcaac cagtaggtga
     4801 atatcgcagt gatgcgctgg cggctgaaga gatactgacg ctggcgaact ggtgcctgtt
     4861 gaactactcc gggctgaaaa cgccagtcgg gagtgcatca tgagtatcct gacccggtgg
     4921 ttgcttatcc cgccggtcaa cgcgcggctt atcgggcgtt atcgcgatta tcgtcgtcac
     4981 ggtgcgtcgg ctttcagcgc gacgctcggc tgtttctgga tgatcctggc ctggattttt
     5041 attccgctgg agcacccgcg ctggcagcgt attcgcgcag aacataaaaa cctgtatacg
     5101 catatcaacg cctcgcgtcc gcgtccgctg gacccggtcc gttatctcat tcaaacatgc
     5161 tggttattga tcggtgcatc gcgcaaagaa acgccgaaac cgcgcagcgg gcattttcag
     5221 gtctgcaaaa tattcgtgga cgttaccatc aatggatgaa cgagctgcct gagcgcgtta
     5281 gccataaaac acagcatctg gatgaaaaaa aagagctcgg tcatttgagt gccggggcgc
     5341 ggcggttgat cctcggtatc atcgtcacct tctcgctgat tctggcgtta atctgcgtta
     5401 ctcagccgtt taacccgctg gcgcagttta tcttcctgat gctgctgtgg ggggtagcgc
     5461 tgatcgtacg gcggatgccg gggcgcttct cggcgctaat gttgattgtg ctgtcgctga
     5521 ccgtttcttg ccgttatatc tggtggcgtt acacctctac gctgaactgg gacgatccgg
     5581 tcagcctggt gtgcgggctt attctgctct tcgctgaaac gtacgcgtgg attgtgctgg
     5641 tgctcggcta cttccaggta gtatggccgc tgaatcgtca gccggtgcca ttgccgaagg
     5701 atatgtcgct gtggccgtca gtggatatct ttgtcccgac ttacaacgaa gatctcaatg
     5761 tggtgaaaaa taccatttac gcctcgctgg gtatcgactg gccgaaagac aaactgaaca
     5821 tctggatcct cgatgacggc ggcagggaag agtttcgcca gtttgcgcaa aacgtggggg
     5881 tgaaatatat cgcccgcacc actcatgaac atgcgaaagc gggcaacatc aacaatgcgc
     5941 tgaaatatgc caaaggcgag ttcgtgtcga ttttcgactg cgaccacgta ccaacgcgat
     6001 cgttcctgca aatgaccatg ggctggttcc tgaaagagaa acagctggcg atgatgcaga
     6061 ctccacacca cttcttctcg ccggacccgt ttgaacgcaa cctgggacgt tttcgtaaaa
     6121 cgccgaacga aggcacgctg ttctatggtc tggtgcagga tggcaacgat atgtgggacg
     6181 ccactttctt ctgcggttcc tgtgcggtga ttcgtcgtaa gccgctggat gaaattggcg
     6241 gcattgctgt cgaaaccgtg actgaagatg cgcatacttc tctgcgtttg caccgtcgtg
     6301 gctatacctc cgcgtatatg cgtattccgc aggcggcggg gctggcgacc gaaagtctgt
     6361 cggcgcatat cggtcagcgt attcgctggg cgcgcgggat ggtataaatc ttccgtctcg
     6421 ataacccgct caccggtaaa ggactgaagt ttgctcagcg gctgtgctac gtcaacgcca
     6481 tgttccactt cttgtcgggc attccacggc tgatcttcct gactgcgccg ctggcgttcc
     6541 tgctgcttca tgcctacatc atctatgcgc cagcgttgat gatcgcccta ttcgtgctgc
     6601 cgcatatgat ccatgccagc ctgaccaact cgaagatcca gggcaaatat cgccactctt
     6661 tctggagtga aatctacgaa acggtgctgg cgtggtatat cgcaccaccg acgctggtgg
     6721 cgctgattaa cccgcacaaa ggcaaattta acgtcaccgc caaaggtgga ctggtggaag
     6781 aagagtacgt cgactgggtg atctcgcggc cctacatctt ccttgttcta ctcaacctgg
     6841 tgggcgttgc ggtaggcatc tggcgctact tctatggccc gccaaccgag atgctcaccg
     6901 tggtcgtcag tatggtgtgg gtgttctaca acctgattgt tcttggcggc gcagttgcgg
     6961 tatcggtaga aagcaaacag gtacgccgat cgaaccgcgt ggagatgacg atgcccgcgg
     7021 caattgcccg cgaagatggt cacctcttct cgtgtaccgt tcaggatttc tccgacggtg
     7081 gtttggggat caagatcaac ggtcaggcgc agattctgga agggcagaaa gtgaatctgt
     7141 tgcttaaacg cggtcagcag gaatacgtct tcccgaccca ggtggcgcgc gtgatgggta
     7201 atgaagttgg gctgaaatta atgccgctca ccacccagca acatatcgat tttgtgcagt
     7261 gtacgtttgc ccgtgcggat acatgggcgc tctggcagga cagctacccg gaagataagc
     7321 cgctggaaag tctgctggat attctgaagc tcggcttccg tggctaccgc catctggcgg
     7381 agtttgcgcc ttcttcggtg aagggcatat tccgtgtgct gacttctctg gtttcctggg
     7441 ttgtatcgtt tattccgcgc cgcccggagc ggagcgaaac ggcacaacca tcggatcagg
     7501 ctttggctca acaatgatga taacgcgatg aaaagaaaac tattctggat ttgtgcagtg
     7561 gctatgggga tgagtgcgtt cccctctttc atgacgcagg cgacgccagc aacgcaacca
     7621 ctgatcaatg ctgagccagc tgtagccgcc cagacggaac aaaatccgca ggtggggcaa
     7681 gtgatgtcag gcgagcaggg agctgatgcc cccatcgtgg cgcagaacgg tccttcgcgt
     7741 gatgtgaagc tgacctttgc gcaaatcgcg ccgccgccgg gcagcatggt gctacgtggt
     7801 attaacccga acggcagcat tgagtttggt atgcgcagcg atgaagtggt gacgaaggcg
     7861 atgctcaact tcgaatacac gccatcgcca tcgttactgc ctgtccagtc gcagttaaag
     7921 gtttatctca atgatgagct gatgggcgtg ctgccagtga ccaaagaaca gttgggtaaa
     7981 aaaacgctgg cgcaaatgcc cattaatcca ctgtttatta ccgacttcaa ccgtgtgcgg
     8041 ctggagtttg tcggccatta tcaggacgtg tgcgaaaacc cggccagcac cacgctttgg
     8101 ctggatgttg ggcggagcag tggactggat ctgacctatc agaccctgaa tgtgaagaat
     8161 gacctgtcac acttcccggt gccattcttt gacccgcgcg ataaccgtac taacaccttg
     8221 ccgatggtct ttgcgggtgc gccggatgtt gggctgcaac aagcatctgc cattgtcgcc
     8281 tcgtggtttg gttcgcgttc tggctggcgt gggcagaact tcccggtgct ctataaccaa
     8341 ttgccggatc gcaatgccat tgtctttgca accaacgaca aacggccgga cttcctgcgc
     8401 gatcatccgg cggtaaaagc cccggtgatt gagatgatta accatccgca gaatccttac
     8461 gtcaaactgc tggtggtgtt tggtcgtgac gacaaagacc tgttgcaggc agcgaaaggt
     8521 atcgctcagg gtaacattct gttccgtggt gaaagcgtgg tagtgaatga agtgaaaccg
     8581 ctgctaccgc gtaagccgta cgatgcgccg aactgggtac gtaccgatcg tccggtcaca
     8641 tttggcgaac tgaaaaccta tgaagaacag ttacaatcca gcggccttga gccagcagcg
     8701 attaacgttt cgctaaacct gccgccggat ctctacctga tgcgcagtac cggcattgat
     8761 atggatatta attaccgcta caccatgccg ccggtgaaag acagttcgcg gatggatatc
     8821 agcctgaata accagttcct gcaatccttc aacctgagca gcaaacagga ggcgaaccgt
     8881 ctgctgctgc gtattccggt attacaaggt ttgctggatg gcaaaacaga tgtctctatt
     8941 ccggcgctga aactgggcgc gaccaaccag ctgcgcttcg actttgagta tatgaacccg
     9001 atgccaggcg gttcggtaga taactgtatt accttccagc cggtgcagaa tcatgtggtg
     9061 attggtgacg attccaccat cgacttctcg aagtattacc acttcatccc gatgccggat
     9121 ctacgcgcct ttgctaacgc gggcttccca ttcagccgga tggcggatct gtcgcaaacc
     9181 atcaccgtga tgccgaaagc gcctaacgaa gcacagatgg aaacgttgct gaatactgtt
     9241 ggttttatcg gcgcacagac gggcttcccg gcgattaatc tgacggtgac cgatgatggc
     9301 agcaccattc agggcaaaga tgccgacatc atgatcatcg gtggtatccc ggacaaactg
     9361 aaagacgata agcagatcga cctattggtg caggcgaccg aaagctgggt gaaaacaccg
     9421 atgcgccaga ccccgttccc cggcattgtg ccggacgaga gcgatcgcgc ggcagaaacc
     9481 cagtcaactc tgacctcttc cggtgcgatg gcggcggtga ttggcttcca gtcgccgtat
     9541 aacgaccagc gcagcgtgat tgcgctgttg gcagatagcc cacgcggtta tgaaatgctt
     9601 aacgatgcgg tgaacgatag cggcaaacgc gccaccatgt tcggttcggt cgcggtgatc
     9661 cgcgagtccg gtatcaacag cctacgtgtt ggcgacgttt attacgtagg tcatctgccg
     9721 tggttcgagc gcttgtggta tgcgctggca aaccatccga ttctgctggc ggtgctggcg
     9781 actatcagtg tgatattgct ggcatgggta ctgtggcgtc tgctgcgaat tattagtagt
     9841 cgtcgtctta acccggataa cgagtaattg aagatgaatg tgttgcgtag tggaatcgtg
     9901 acgatgctgt tgttggctgc ctttagtgtt caggcagcct gtacctggcc tgcctgggag
     9961 cagtttaaaa aggattacat cagtcaggaa gggcgcgtca tcgaccccag cgacgcgcgc
    10021 aaaatcacca cctccgaagg gcaaagttac ggtatgttct ttgccctggc ggctaacgac
    10081 cgtgcagctt tcgataatat tctcgactgg acgcagaaca atctcgctca gggttcttta
    10141 aaagaacgtt tgcccgcctg gctgtggggc aagaaagaga acagtaagtg ggaagtgctg
    10201 gacagcaatt cggcctccga tggtgatgtc tggatggcct ggtcgttgct ggaggcgggg
    10261 cgtttgtgga aagagcagcg ttataccgac atcggcagcg cgttgctaaa acgtatcgcg
    10321 cgggaggaag tggtgacggt gcctgggctg ggttccatgt tgttaccggg caaagtgggt
    10381 tttgctgagg ataacagctg gcgttttaac cccagctaca tgccgccgac gctggcgcag
    10441 tatttcaccc gctttggcgc gccgtggacc acgctgcgcg aaaccaatca acatttattg
    10501 ctggaaaccg ccccgaaagg cttttcgcca gactgggtgc gctatgagaa agacaaaggc
    10561 tggcagctaa aagccgaaaa aacattgatc agcagctacg acgctatccg cgtttacatg
    10621 tgggtaggca tgatgcctga cagcgatccg caaaaagcgc ggatgctcaa ccggtttaaa
    10681 ccgatggcga cattcactga gaaaaacggt tatccgccgg aaaaagtgga tgtggctacg
    10741 gggaaagcgc agggtaaagg accggtcggt ttttctgccg ccatgctgcc ttttttacaa
    10801 aaccgcgatg cgcaggcagt tcagcgccag cgcgtggccg ataactttcc cggcagcgat
    10861 acctattaca actatgtgct gaccctgttt ggacaaggct gggatcaaca ccgtttccgc
    10921 ttctcgacaa aaggtgagtt attacctgac tggggccagg aatgcgcaaa ttcacactaa
    10981 acatattcac gctttccctc ggtctggccg tcatgccgat ggtcgaggca gcaccaacca
    11041 tcagcagacc ctgcggatgg acagcggcaa cactaatgcc gtgcgcgggc tggcgaatat
    11101 ttaccgccag cagtcgccag aaaaagctga agcgtttatc gcctcgctct ctgccagcca
    11161 gcggcgcagc attgatgata tcgaacgcag cctgcaaaac gaccgtctgg cacagcaggc
    11221 agaggtactg gaaaaccagg gcaaatgggc gcaggcggca gcacttcagc ggcaacgact
    11281 ggcgctgggc cccggcagcg tatggattac ttaccgactt tcgcaggatc tctggcaggc
    11341 cggacaacgc agccaggccg atacgttaat gcgcaatctg gcgcagcaga agccgaacaa
    11401 cccggagcag gtttacgctt acgggctgta cctctcaggt cacaaccagg acagagcggc
    11461 gctggcgcat atcaacagcc tgccgcgcgc gcagtggaac agcaatattc aggagctggt
    11521 taatcgactg caaagcgatc aggtgctgga aaccgctaat cgcctgcgag aaagcggcaa
    11581 agaggctgaa gcggaagcga tgctgcgtca gcaaccacct tccacgcgta ttgacctcac
    11641 gctggctgac tgggcgtaac aacgacgtga ttacaccgcc gcccgcactg catatcagaa
    11701 tgtcctgacg cgggagccaa ctaacgccga tgccattctt ggtctgacgg aagtggatat
    11761 tgctgccggt gacaaagcgg cggcacgtag ccagctggcg aaactgcctg ccaccgataa
    11821 cacctcgctg aacactcagc ggcgcgtggc gctggcacag gcgcagcttg gagataccgc
    11881 agcggcgcag cagacgttta ataagttgat cccgcaggca aaatctcagc caccgtcgat
    11941 ggaaagcgcg atggtactgc gcgacggtgc gaagtttgaa gcgcaggcgg gcgatccaaa
    12001 gcaggcgctg gaaacctaca aagacgccat ggtcgcatcc ggtgtgacta cgacgcgtcc
    12061 gcaggataac gacaccttta cccgactgac ccgtaacgac gagaaagatg actggctgaa
    12121 acgcggcgtg cgcagcgatg cggcggacct ctatcgccag caggatctta acgtcaccct
    12181 tgagcacgat tactggggtt cgagcggcac cggtggttac tccgatctga aagcgcacac
    12241 taccatgttg caggtggatg cgccgtattc tgacgggcgg atgttctttc gcagtgattt
    12301 cgtcaatatg aacgtcggca gtttctccac taatgccgat ggcaaatggg atgacaactg
    12361 gggcacctgt acattacagg actgtagcgg caaccgcagc cagtcggatt ccggtgccag
    12421 cgtggcggtc ggctggcgaa atgacgtctg gagctgggat atcggtacca cgccgatggg
    12481 cttcaacgtg gtggatgtgg tcggcggcat cagttacagc gatgatatcg ggccgctggg
    12541 ttacaccgtt aacgcccacc gtcggcccat ctccagttct ttgctggcct ttggtgggca
    12601 aaaagactcc ccgagcaata ccgggaaaaa atggggtggc gtacgtgccg acggtgtggg
    12661 gctaagtctg agctacgata aaggtgaagt aaacggcgtc tgggcatcgc ttagtggcga
    12721 ccagttaacc ggtaaaaatg tcgaagataa ctggcgcgtg cgctggatga cgggctatta
    12781 ctataaggtc attaaccaga acaatcgccg cgtcacaatc ggcctgaaca acatgatctg
    12841 gcattacgac aaagatctga gtggctactc actcggtcag ggcggttact acagtccgca
    12901 ggaatacctg tcgtttgcca taccggtgat gtggcgggag cgcacggaaa actggtcgtg
    12961 ggagctgggg gcgtctggct cgtggtcgca ttcacgcacc aaaaccatgc cgcgttatcc
    13021 gctgatgaat ctgatcccga ccgactggca ggaagaagct gcgcggcaat ccaacgatgg
    13081 cggcagcagt cagggcttcg gctacacggc gcgggcatta cttgaacgac gtgttacttc
    13141 caactggttt gttggtacgg caattgatat ccagcaggcg aaagattacg cacccagcca
    13201 tttcctgctc tacgtacgtt attccgccgc cggatggcag ggtgacatgg atttaccgcc
    13261 gcagccgctg ataccttacg ccgactggta agttttcaga tagcgcctct cttaatgccg
    13321 ctgcgatcgg gtatactcgg gcggcaatct gggatttccg gggggagaca atttgcgcgt
    13381 aagtcgctcg ttaacaatca agcagatggc aatggtggca gccgttgtcc tggtgttcgt
    13441 ttttattttt tgcaccgttt tgctgttcca tctggtccag cagaatcgct ataacacggc
    13501 tacgcaactg gaaagcattg ctcgctctgt ccgcgaaccc ttatcttcag ctattttgaa
    13561 aggcgatatt cccgaagcgg aagctattct tgccagcatt aaaccggcag gcgtggtcag
    13621 ccgtgccgat gtagtgctgc ctaaccagtt ccaggcgctg cgtaaaagtt ttattccaga
    13681 gcgtccggtg ccggtaatgg ttactcgcct gtttgagcta ccggttcaaa tctcgctggg
    13741 cgtctactcg ctcgaacgtc cggcaaatcc gcagccaata gcctatctgg tgctacaggc
    13801 ggattccttc cgtatgtata agttcgtgat gagcactctc tcaacgttag tgaccattta
    13861 cttacttttg tcgcttatcc tgaccgtcgc catcagctgg tgcattaacc gcctgatttt
    13921 gcatccgtta cgcaatattg ctcgcgaact taacgccatc ccagcccagg agcttgttgg
    13981 tcaccaactg gcattaccgc gtctgcatca ggacgatgaa atcggtatgt tggtgcgcag
    14041 ttacaacctc aaccagcaat tgctgcagcg ccattatgaa gaacagaacg aaaatgcgat
    14101 gcgcttcccg gtgtcggatt tgccgaacaa agccttgctg atggagatgc tggagcaggt
    14161 tgtcgcgcgt aaacaaacca ccgcgctgat gatcatcacc tgtgaaaccc tgcgtgatac
    14221 tgcgggcgtg ctgaaagagg cgcaacgaga aattctgctg ctgacgctgg tggaaaaact
    14281 caaatcggta ctgtcgccac gtatgatcct cgcgcagatt agcggttatg actttgctgt
    14341 cattgccaac ggtgtacagg aaccgtggca cgcaatcact ttgggtcagc aagtactcac
    14401 tatcatgagc gagcgcctgc cgattgaacg tattcaactc cgtccgcact gtagcattgg
    14461 cgtggcgatg ttctacggcg atctcaccgc cgaacagctt tacagtcgcg ctatttctgc
    14521 ggcatttacc gctcgccata aaggcaagaa tcagattcag ttctttgatc cgcagcagat
    14581 ggaagcagcc cagaagcggt tgacggaaga gagcgatatc cttaatgcac tggaaaatca
    14641 tcagtttgca atttggttac agccacaggt cgagatgacc agcggtaaac tggtcagtgc
    14701 ggaagtgtta ctgcgtatcc agcaaccgga tggcagttgg gacctgccgg atggcttaat
    14761 cgatcgcatt gagtgctgtg ggctgatggt taccgtcggt cactgggtgc tggaagagtc
    14821 ctgtcgattg cttgcagcct ggcaagagcg cggcattatg ctgcccttgt cggtaaactt
    14881 ctctgcgctg caactgatgc acccgaatat ggtggcggat atgctggaac tgttaacccg
    14941 ctatcgcatt cagccgggaa cactgattct ggaagtgaca gaaagccgac gtattgacga
    15001 ccctcatgct gcggtggcaa tcctccgtcc gctgcgcaat gccggagttc gggtggcgct
    15061 ggatgatttc ggcatgggct acgcagggct gcgtcagctg cagcatatga aatcgttgcc
    15121 aatcgacgta ctgaaaatcg acaaaatgtt tgttgaaggc ttgccggaag atagcagcat
    15181 gattgctgca attatcatgc tggcgcagag cctgaactta caaatgattg ccgaaggcgt
    15241 ggagactgaa gcacaacgag actggctggc aaaagcgggc gttggtattg cccagggctt
    15301 cctttttgct cgcccactcc ctattgaaat cttcgaagag agttacctgg aagaaaagta
    15361 gctaccccaa actgattaca aaactttaaa aagtgctggt ttgtgcgagc cagctcaaac
    15421 tttttaacct ttttgtttca attatgatcc aggtacattt ctgtgatgtt gtctgggtgt
    15481 tattttaagg ccgcaggtac cccccataac cttacaagac ctgtggtttt actaaaggac
    15541 accctatgaa aacctctctg tttaaaagcc tttactttca ggtcctgaca gcgatagcca
    15601 ttggtattct ccttggccat ttctatcctg aaataggcga gcaaatgaaa ccgcttggcg
    15661 acggcttcgt taagctcatt aagatgatca tcgctcctgt catcttttgt accgtcgtaa
    15721 cgggcattgc gggcatggaa agcatgaagg cggtcggtcg taccggcgca gtcgcactgc
    15781 tttactttga aattgtcagt accatcgcgc tgattattgg tcttatcatc gttaacgtcg
    15841 tgcagcctgg tgccggaatg aacgtcgatc cggcaacgct tgatgcgaaa gcggtagcgg
    15901 tttacgccga tcaggcgaaa gaccagggca ttgtcgcctt cattatggat gtcatcccgg
    15961 cgagcgtcat tggcgcattt gccagcggta acattctgca ggtgctgctg tttgccgtac
    16021 tgtttggttt tgcgctccac cgtctgggca gcaaaggcca actgattttt aacgtgatcg
    16081 aaagtttctc gcaggtcatc ttcggcatca tcaatatgat catgcgtctg gcacctattg
    16141 gtgcgttcgg ggcaatggcg tttaccatcg gtaaatacgg cgtcggcaca ctggtgcaac
    16201 tggggcagct gattatctgt ttctacatta cctgtatcct gtttgtggtg ctggtattgg
    16261 gttcaatcgc taaagcgact ggtttcagta tcttcaaatt tatccgctac atccgtgaag
    16321 aactgctgat tgtactgggg acttcatctt ccgagtcggc gctgccgcgt atgctcgaca
    16381 agatggagaa actcggctgc cgtaaatcgg tggtggggct ggttatcccg acaggctact
    16441 cgtttaacct tgatggcaca tcgatatacc tgacaatggc ggcggtgttt atcgcccagg
    16501 ccactaacag tcagatggat atcgtccacc aaatcacgct gttaatcgtg ttgctgcttt
    16561 cttctaaagg ggcggcaggg gtaacgggta gtggctttat cgtgctggcg gcgacgctct
    16621 ctgcggtggg ccatttgccg gtagcgggtc tggcgctgat cctcggtatc gaccgcttta
    16681 tgtcagaagc tcgtgcgcta actaacctgg tcggtaacgg cgtagcgacc attgtcgttg
    16741 ctaagtgggt gaaagaactg gaccacaaaa aactggacga tgtgctgaat aatcgtgcgc
    16801 cggatggcaa aacgcacgaa ttatcctctt aatctcacca cttatgcccg cactcccttc
    16861 cccgagtgcg ggtgtttgcg cataattgac ccctgtgcct gaacttacca cctgggtgag
    16921 ttcaggttta tttcacttct tccgtgacat tttcatgttc ttgcggtcta acacgaagtg
    16981 tttttaacgt catttagact gaccgacaac gggcagtttt tttattacca ggatagttga
    17041 tcaggggttc acatgcaggg cataaaaatt cgacttttag cgggcggttt gctgatgatg
    17101 gccactgctg gctatgtgca ggcagatgcg ctccagcctg atccagcatg gcaacagggg
    17161 acgctttcca acggtttaca gtggcaagtg ctgaccaccc cccagcgtcc cagcgatcgt
    17221 gttgaaattc gcctgctggt taataccggt tcgctcgccg aaagtacaca acagagcggt
    17281 tacagtcacg ccatccctcg tattgcgcta acgcaaagcg gtggccttga cgcagcacag
    17341 gcgcgttcat tgtggcagca ggggatcgac cctaaacgcc cgatgccgcc ggtaattgtc
    17401 tcttatgaca ccacgctgtt taatctgagt ttgcccaata accgtaacga tttgctgaaa
    17461 gaagcgctct cttatctggc aaatgccact ggcaaattga ccatcacacc agaaaccatc
    17521 aaccacgcgc tgcaaagtca ggacatggtg gcaacctggc ctgccgatac taaagagggc
    17581 tggtggcgct atcgtctgaa agggtcaacc ttgttaggtc acgatcctgc cgatccgctg
    17641 aaacaacccg ttgaagcgga aaaaattaaa gatttctatc agaaatggta caccccggat
    17701 gcaatgacgc tactggtggt gggaaacgtg gatgcgcgct cggttgtcga ccaaatcaac
    17761 aaaacgtttg gcgaactgaa aggcaaacgt gaaacgccag ctccggtgcc gacgctttct
    17821 ccgctgcgtg cggaagcggt gagtattatg actgacgcgg tgcgtcagga ccggttatct
    17881 atcatgtggg atacgccgtg gcagccgatt cgtgaatcag ccgcactgct gcgctactgg
    17941 cgtgcggacc tggcccgcga ggcgctgttc tggcatgttc agcaagcgtt aagcgccagt
    18001 gacagcaaag acatcggtct tggatttgac tgccgtgtgc tgtatctgcg tgcgcagtgt
    18061 gccatcaaca tcgaatcacc aaacgacaag ctgaacagca accttaatct ggtggcgcgt
    18121 gaactggcga aggttcgcga taaaggtctg ccggaagaag agttcaatgc gttagtggcg
    18181 caaaagaaac tggagctgca gaaactgttt gccgcctatg cacgggctga taccgatatt
    18241 ctgatgggcc agcggatgcg ttcgttgcaa aatcaggtcg tcgatatcgc gccggagcag
    18301 tatcagaaac tgcggcagga tttccttaac agcctgactg tggagatgtt aaatcaggat
    18361 ctgcgtcagc agttgtcgaa tgatatggcg ttaatactgc tgcagccgaa aggcgagccg
    18421 gaatttaaca tgaaagcgtt gcaggcggcc tgggatcaaa tcatggcccc atctaccgcc
    18481 gctgcgacca cctctgtcgc cacggatgac gtacatcctg aagtgacgga tattccacct
    18541 gcacagtaat gcctgatggt gatgagttag cacaaccatc aggtatttat ggatgagctg
    18601 gatagagggg taacacttta tccagccttt tgcatatgct gcgtttacgc tggcatcgcc
    18661 tcacgcggga taatcgcgcc gcgatactga ataacggtac ttgcggtcag gtgcccgcgt
    18721 ttcgccgcgt tttccgcact gccgcccgtc aaacgtaccg ccagataacc ggcgctgaaa
    18781 gagtcgccag ctgcggtggt atcgatcact ttttctttcg gcagtttcac cgccggaaca
    18841 tcaactaacg cttcaccagc aatggacacc aggcaagaat ccgccccgcg tttcaccacc
    18901 acttctttca cgcccgcgtt atgggtgcgc gcaatgacgt cttccaccgg ctgttgaccc
    18961 cacagcgcgt cttcgtcgtc cagcgtcagg aaggcgatat ccgtgcattc cagcatttgc
    19021 tggtacacct gctgtgtctc ttctttgctg gcccacaggc gcggacgata gttattgtcg
    19081 aaaatcactt ttccgccttt ggcgcggcat tcgcgcagca gggaaagcag cttttcgcgg
    19141 ctggtcgggc ttaagatcgc caggctaatc ccgctcaggt agagataatc gaaattcgcc
    19201 agctcttcgc aaatcgccgc agactgctcg ctcgccagcc agaatttggc ggcggcttcg
    19261 ttacgccagt agtagaacgt acgttcgccg gtgctgtcgg tttcaatgta gtaaaggccc
    19321 ggaagacggt tttccatccg ttgggtcagg gaagtatcaa cgttctcgcc gtgccaggcg
    19381 tccagcatct gctggctaaa actgtccgtt cccagcgccg ttacgtaatg aacggttaat
    19441 gccgcaggat cgacctgacg ggcgatatag acggaagtgt tcagggtatc gccgccgaaa
    19501 ccgcgcttaa cgtccgcgcc tttctcggaa agctcaatca tgcattcgcc aatcacggca
    19561 atctttttgg acatagtcgt gaacctgatc tgtaaaaaat tagcgttagt ttgcgctgtg
    19621 gtggtttgct ggtcaaccat attaaaacag tgttccatta tttttttgag ccagaacgta
    19681 ttcctgaaag attccgttgt ggagtgagga aatttacccc ggccaggcgg taaagttctg
    19741 cccttacgcg ccgataatct ttgtcgagtc cgggcagcat cacttttaaa cacaggacat
    19801 ctttgatgat aaggcaggtt atccagcgaa taagcaaccc tgaagcaagc atcgagagct
    19861 tgcaggaacg gcgtttttgg ttgcagtgtg agcgtgctta cacctggcag ccgatctatc
    19921 aaacatgcgg gcggttaatg gccgtggagc tattaacggt ggtcacgcat cccttgaacc
    19981 cttcgcaacg cctgccgccg gatcgctatt ttactgaaat caccgtcagc catcggatgg
    20041 aggttgtgaa agagcagatt gatttgctgg cgcaaaaagc cgacttcttt gtagagcacg
    20101 gcctgctggc atcggtcaat attgatggcc ctacgctcat cgccctgcgt cagcaaccaa
    20161 aaatcctgcg ccagattgag cgtcttccct ggctgcgttt cgaactggtg gagcatatct
    20221 gtctgccgaa agattcaacc tttgcctcga tgtgtgaatt tggcccgctg tggctggatg
    20281 attttggtac cgggatggca aatttctctg cactaagtga agtgcgttat gactacatca
    20341 aaatcgcgcg agaactgttt gtgatgctgc gtcagtcgcc ggaaggccgc acactctttt
    20401 ctcagctttt acatctaatg aatcgctatt gtcgcggggt gattgtcgag ggcgtagaaa
    20461 caccggaaga gtggcgtgat gttcagaact cgcccgcatt cgccgcacaa ggctggtttc
    20521 tttcacgccc ggcaccgatg gaaacgctga atacggcggt tctggcgcta taagctgcct
    20581 cattttccgc cgggctggac tatctttagg actggcacag gaagaatatg aggaagcgaa
    20641 cgatgagcaa ggcaggcaaa ataaccgctg cgatttcagg ggctttcttg ttgttgattg
    20701 tcgtggcgat cattttgatt gcaacttttg actggaatcg actcaaaccg accatcaacc
    20761 agaaagtctc tgcggagttg aatcgtccgt tcgctatccg tggcaatctg ggcgtggtgt
    20821 gggagcggca aaaacaagaa actggctggc gcagctgggt gccgtggcct catgtacatg
    20881 cagaagacat cattcttggt aatccaccgg atattcccga agtcacgatg gtgcatttgc
    20941 cgcgagtaga ggcaacgctg gcaccgctgg cgctgctgac caaaacggtc tggctgccgt
    21001 ggatcaagct cgaaaagccc gacgcgcgcc tgattcgcct ctctgaaaag aacaataact
    21061 ggacgtttaa tcttgccaac gatgacaaca aagacgcgaa tgcaaagccg tcggcatggt
    21121 cgtttcggct ggataatatt cttttcgatc aagggcggat cgccattgat gacaaagtaa
    21181 gcaaagcgga tctggaaatt tttgttgatc cgttaggcaa gccgctgcca ttcagcgaag
    21241 ttactggatc gaaaggtaaa gcggataaag aaaaggtggg cgattacatt tttggcctga
    21301 aggcgcaggg acgttataac ggtgaaccgc tcacgggtac gggaaaaata ggcggtatgc
    21361 tggcgctgcg tggcgaaggg acgccgtttc cggtacaggc tgatttccgt tcaggtaata
    21421 cccgtgttgc ttttgatggc gtcgtgaatg acccaatgaa gatgggcggt gtcgatttac
    21481 ggcttaaatt ttctggcgat tcactgggtg atctctatga actgacgggc gttctgctgc
    21541 ccgatacccc gccgtttgaa acggatggtc ggctggtagc gaaaatcgac actgaaaaat
    21601 cgtcggtctt tgattatcgc ggttttaatg ggcgaattgg cgatagcgat atccacggtt
    21661 ctctggtcta caccaccgga aagccacgac caaaactgga aggtgatgtc gagtcgcggc
    21721 aattgcggct ggcggacctg ggaccgttga ttggcgttga ttccgggaaa ggggcagaaa
    21781 agtcgaaacg gtctgaacag aagaagggcg aaaaaagcgt tcagcctgcg ggcaaagtgc
    21841 tgccttatga ccgcttcgaa accgataaat gggacgttat ggatgccgat gttcgcttca
    21901 aagggcggcg cattgagcat ggcagtagcc tgccgattag cgatctttct actcatatca
    21961 tcctcaaaaa tgctgacctg cgcctgcaac cgctgaaatt tggcatggcg ggcggcagca
    22021 ttgcggcgaa tattcatctg gaaggcgata aaaagccgat gcaggggcgg gcagatattc
    22081 aggctcgtcg actgaaactg aaagaactga tgcccgatgt ggaactgatg cagaagacgc
    22141 tgggggaaat gaacggtgac gcggaactac gcggtagcgg taactcggtg gcggcgcttt
    22201 taggcaacag taacggcaac ctgaaactgt tgatgaatga cgggctggtg agccgcaacc
    22261 tgatggagat tgttgggctg aatgtcggca actacattgt cggtgcgata tttggtgacg
    22321 atgaggtgcg ggtgaactgc gcggcggcga atctgaatat tgccaacggc gtggcgcgcc
    22381 cgcagatttt tgctttcgat actgagaacg cgttgattaa tgttaccggc acggcaagtt
    22441 ttgcttcgga acagctggat ttgactattg atccggagag taaagggatt cggattatca
    22501 cactgcgttc gccgctgtat gtgcgtggga cgtttaaaaa tccgcaggct ggggtgaaag
    22561 ccggaccgct gattgcccgt ggtgctgttg ctgcggcact ggcaacgctg gtaacaccgg
    22621 cggcggcgtt actggcactg atctcacctt ccgaagggga ggctaatcag tgtcggacga
    22681 tattgtcgca gatgaagaag tgatgataat tgccggatgt ctgacatccg gcaattaaac
    22741 agatttagat ttttggcaaa gtgtgctttg ttcatgccgg atgcggcgtg aacgccttat
    22801 ccggcctaca aatccttgtg aattcaatat attgtatgga aattcagtcc tgataagcgt
    22861 agcgcatcag gcttttcaca cttattacaa cgactgatgt cgcgtctcat gggtcagcag
    22921 cagggcgatt aacgtcaagc cagccatcgc cgccagatat aaccccaccg cacctaaccc
    22981 gtagttagcc tgcaaccagg ctgcgatata cggcgcaacg gaagccccga gaatcgacga
    23041 tacgttgtaa gagaacgatg ctccggtgta acgcacttct gtcggaaaca gctctggtaa
    23101 cagcgcaccc atcggcccga aggtcagccc catcagactt aaccccagca gcaggaaggc
    23161 aaaaaccaga atcgggttgc cagaaccgag cagcgggtta aaggcgaaca gcgcgaacag
    23221 gatgatcagc gtggtgatga tcaccatgct tttacggcga ccaaaggcat cagccagtaa
    23281 tccggcgact ggcaccatca cgccaaaacc aataactgcc atcatcagca tccacaacac
    23341 ttcgttacgc ggcaggccaa gcccaactgg cgcggcggcg gtactgaagg tcatagagta
    23401 gaccgtcatg atgtaaaaca gcgtataggt tgccagcata atgaacgtac ccagtacggt
    23461 tacgcgaaca tgtttggtca gcagcgtacc cagcgggatc ttcacctgtt ttttcgcttt
    23521 agcgactttc tcaaacaccg gcgactcatg cagcgacacg cgaacataca ggccgataat
    23581 gaccagcacc gccgagaaga taaatggcac acgccagccc cagctcataa actgctcatc
    23641 ggtcagtagc caggaaagca gcaagaaagt gccgttggca aagaagaagc caatcggtgc
    23701 gcctagctgt gggaaggagc catacagtgc acgtttgcgc ggtggggcgt tttcagtcgc
    23761 cagcagcgcc gcaccgcccc attcaccgcc taagcccaga ccctgaccaa atcgagccag
    23821 cgccagcagc agtggggcga aaataccaat cgtggcatag cccggcagca gaccaatcac
    23881 cacggtcgaa atccccatcg ttagcaacga ggcgaccagc gtcgctttac gcccaacgcg
    23941 atcgccaaaa tgaccaaaaa cggcagagcc aatggggcgc gcgacgaagg cgatggcgaa
    24001 ggtggcgagc gactgtagcg ttgctgccgt tggatcgccc tgcgggaaga agatatgcgg
    24061 aaacacaata acggccgcag tggcgtaaat gtaaaagtcg aagaactcaa tggccgtgcc
    24121 aatgagagag gcgacaagga ctttattacg cgagttgatc ggcgtgtatt cttgttcgtg
    24181 gtcgagtgtt gtggctgttg cttgcataat cttttcttat tttttggcta acgaatagcc
    24241 atattacgca cagcaaaagt gatatttcaa tgtgtaacaa atcgcggaaa tggtcaaaaa
    24301 ccaggcaaaa aggggataat gtttaggcca gtgatttttc ttctatgaaa tgcttcacat
    24361 aattttataa atagtggata aaactggttt caggttaaat aaaagttacc aactggattt
    24421 atatgctggg gttatgagtt ggagtgaaat tgtttgctcc caaatagatg gctgaagagg
    24481 ctctggggaa gagtttcaag aggttatgtg cgcattatag ccggatgcga tgctggcgca
    24541 tcttatccgg cctacgggga gccaggccga ataaaatcgt atgccggata aggcgtttac
    24601 gccgcatccg gcctatttta aggctgcgtt ttccctggca ttcgcgggtc gtctttatat
    24661 tcggcggtgg caatccacgc cgcgcaaaac agcgtcaaac gggcgaagaa gtagaaaaat
    24721 gccatcagcc ccagcacgga accaaatgct gcgccagagg gggatttcat cagcgatggc
    24781 agggtgtagg tcatcacgat tttaatcact tcaaaaccaa tagcagccag aaatgttccg
    24841 cgaatcaggg cttttttgcg cggacggtgg cgcggcaacc gccagaagat ccagaagaaa
    24901 agcagatagt tggcgaagat ggaaatcgcc aggccaatca atcgccacgt cggtttcagc
    24961 cactcaatgc tgttcaggtg cagggcgcta ataatcattt gctgcgccga accggcaacc
    25021 gaggtgatcg aaagcgtcac aatcagcgca atcaacaaac caatcagtga aataaaatca
    25081 cgcagatatt ttacccagaa cttctcctga tcttgcggcg agcgttccca gacatcgcgc
    25141 gactgggcac gaatcgcttc acgcaggtta cccatccagt tgatgccgga ataaagtgcc
    25201 accgccaggc cgacaagccc tacagtcgta cgctgctgaa cggcggtgtt gatggtgttt
    25261 ttcaacgtgg cggctagtgt cggatcgctg atgttttgca gaattttgtc gaagatatcc
    25321 tgtagcaaca tcggatggga ggccagcaca aagccccctg cggcaaacga caccatcaaa
    25381 atcgggatca tcgacaaaaa tgagaaatag gtgatagccg caccaaactg gttacccagc
    25441 cgatcgttaa agcgttctgt cgcgcgaata aggtgcgcaa taaccggttg gcgttggact
    25501 ttttcggcag tggtggtgac ggtttccagc gcctggctaa ctttgccgcc tttctcgcta
    25561 ttatccaact gcttaatcgg ctcgtgctcc agatcctggg tgggacgttt gatctcgttt
    25621 tcctgcgtca tccgctcttt tgtccttctg tcgtggtcat tcttaaaaag tatagtcggt
    25681 cagtccaggt gctctttcat tacgccagcc agccactgca taaacaggtt tacacgccgg
    25741 gaaagctccc gacgctgcgg gtaaaccagc gaaagggaga gcggctcggc acggtagcct
    25801 ggcaacactt caataagccg cccggcacgc agggcttcgc gcacggcaat gcgcgggatc
    25861 tgaataatcc ccagaccggc aagaccggcg gcgagatagt tttcgctact gtttaccgtc
    25921 aacatgccgc cagacttaaa ccactggacg ccattaacgc tggcaacctc aaaacctaac
    25981 ggatgtacac ccaggtgcgg tgtataacgc actattgcgt gtgaagtcag atcatcgggg
    26041 ctttgcggat aaccaaagcg cgtcagatag tgcggactgg cacagttgac catggttagt
    26101 ttgccgaggg gacgggcgat aacgccatct tccggtaacg cgccagtgcg tatcacgcaa
    26161 tcaaaaccat catgaagaat atctaccgga cggttatggc tactcagttc cagctcaatt
    26221 cccggatgct gatagagaaa ttccgacaga cgcggcagta acaggttttt cgcgattcct
    26281 gacgggatgt cgatgcgtaa tttaccgctg atactggtgg catcctgttg aaacagaccg
    26341 tccagttcgc tgagattact caacacatct tttgcccgtt gataataggt catgccttct
    26401 ggcgtgagtt tgacccgtcg cgtagtgcgc tggagaagct gggtgccaag ttgatgttcc
    26461 agtgcctgta tctggcgcga aacacttccc tttggcaaag caaagaaatc cgccgcgcgg
    26521 gaaaaacttt ccagctccgc gactttgatg aacaactgca ttgcgtgaat tttatccatt
    26581 tctttgaccg attgttgttc acagcgaaac agtaacgcgc atatcgcatt gtttattgtt
    26641 ttttctaaca tctaatgccg ggtttcacaa tgttgtcatg acgaatgtgg cgatttctca
    26701 gcaggaaaag ggcggaatag ctttatgaga aaggtgaatc cccttaaaca gagcgtattt
    26761 ttcagtgatt ccctttaaag tgaatagagg acgtattggc tatttgtgct tatttttgtc
    26821 tcattgccat ttgttatttt ggcttgtagt cgcggaattt catttttaat tgcatgcgta
    26881 ctaaccacca atataaggaa atcctcaaaa ttgttttaaa tagtgccctc gacatgtaat
    26941 ttcaggcgtt tagctgtaac tatttatatc ggtaactatt ttctacgcgt catctctgta
    27001 gaatggccac cgccaaatgg cgaattctta ttttttctcg cgggtaatac ccttccacta
    27061 cttaagtctt tttaacgtat tcaggtggag attcagttat gcaaatagtc atgtttgaca
    27121 ggcagtcaat atttattcat ggaataaaaa tcagtttaca gcagcgtatt ccaggagtga
    27181 gtattcaggg ggtcagtcag gcagacgagt tatggcaaaa gctggaaagt taccctgaag
    27241 ccttagttat gctcgatggc gatcaggatg gtgagttttg ctactggttg ctgcaaaaaa
    27301 ccgtggtgca gtttcctgag gttaaggtgt taatcacggc gacggattgc aataaacggt
    27361 ggttacagga agttatccat tttaatgtgc tggccattgt gcctcgcgat tcaaccgtcg
    27421 agacatttgc gttggcggta aatagtgcgg cgatggggat gatgtttctg cctggagact
    27481 ggcggacgac gccggaaaag gacattaaag atctcaaatc gttaagcgcc cgccagcggg
    27541 agattttaac catgttagcg gcgggcgaat caaataagga ga
//
LOCUS       DAJTQY010000053        27535 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.053, whole genome shotgun sequence.
ACCESSION   DAJTQY010000053 DAJTQY010000000
VERSION     DAJTQY010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27535
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.053"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            319..1617
                     /gene="kgtP"
                     /locus_tag="OO349_002293"
     CDS             319..1617
                     /gene="kgtP"
                     /locus_tag="OO349_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HCR5867343.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1614..1937)
                     /locus_tag="OO349_002294"
     CDS             complement(1614..1937)
                     /locus_tag="OO349_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HCR5867344.1"
                     /translation="MRILFVCSLLLLSGCSHMDNDSWSGQDKAQHFIASAILSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="OO349_002295"
     CDS             complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="OO349_002295"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HCR5867345.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIRERTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3452..6112)
                     /gene="pat"
                     /locus_tag="OO349_002296"
     CDS             complement(3452..6112)
                     /gene="pat"
                     /locus_tag="OO349_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HCR5867346.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRNHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLARREES"
     gene            complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="OO349_002297"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="OO349_002297"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HCR5867347.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="OO349_002298"
     CDS             complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="OO349_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HCR5867348.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7537..8574
                     /locus_tag="OO349_002299"
     CDS             7537..8574
                     /locus_tag="OO349_002299"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCR5867349.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVIQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8622..9311)
                     /gene="ung"
                     /locus_tag="OO349_002300"
     CDS             complement(8622..9311)
                     /gene="ung"
                     /locus_tag="OO349_002300"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCR5867350.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAECE"
     gene            9616..9999
                     /gene="grcA"
                     /locus_tag="OO349_002301"
     CDS             9616..9999
                     /gene="grcA"
                     /locus_tag="OO349_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HCR5867351.1"
                     /translation="MITGIQITKAANADLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="OO349_002302"
     CDS             complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="OO349_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HCR5867352.1"
                     /translation="MTPILLSAFWTYTLITAMTPRPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10745..11626
                     /locus_tag="OO349_002303"
     CDS             10745..11626
                     /locus_tag="OO349_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5867353.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQALNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPHFAVEKEIESGELIELPFGEQSQTITAMCAHYAGKAVS
                     PAMHIFIQCVEECFVVA"
     gene            complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="OO349_002304"
     CDS             complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="OO349_002304"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HCR5867354.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13127..13864
                     /gene="trmN"
                     /locus_tag="OO349_002305"
     CDS             13127..13864
                     /gene="trmN"
                     /locus_tag="OO349_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HCR5867355.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="OO349_002306"
                     /pseudo
     CDS             complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="OO349_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
     gene            15727..15882
                     /gene="rseD"
                     /locus_tag="OO349_002307"
     CDS             15727..15882
                     /gene="rseD"
                     /locus_tag="OO349_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HCR5867356.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            15879..16454
                     /gene="rpoE"
                     /locus_tag="OO349_002308"
     CDS             15879..16454
                     /gene="rpoE"
                     /locus_tag="OO349_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HCR5867357.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            16487..17137
                     /gene="rseA"
                     /locus_tag="OO349_002309"
     CDS             16487..17137
                     /gene="rseA"
                     /locus_tag="OO349_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HCR5867358.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQSATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            17137..18093
                     /gene="rseB"
                     /locus_tag="OO349_002310"
     CDS             17137..18093
                     /gene="rseB"
                     /locus_tag="OO349_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HCR5867359.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            18090..18569
                     /gene="rseC"
                     /locus_tag="OO349_002311"
     CDS             18090..18569
                     /gene="rseC"
                     /locus_tag="OO349_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HCR5867360.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            18767..20566
                     /gene="lepA"
                     /locus_tag="OO349_002312"
     CDS             18767..20566
                     /gene="lepA"
                     /locus_tag="OO349_002312"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HCR5867361.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            20582..21556
                     /gene="lepB"
                     /locus_tag="OO349_002313"
     CDS             20582..21556
                     /gene="lepB"
                     /locus_tag="OO349_002313"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCR5867362.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            21828..22508
                     /gene="rnc"
                     /locus_tag="OO349_002314"
     CDS             21828..22508
                     /gene="rnc"
                     /locus_tag="OO349_002314"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HCR5867363.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            22505..23410
                     /gene="era"
                     /locus_tag="OO349_002315"
     CDS             22505..23410
                     /gene="era"
                     /locus_tag="OO349_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HCR5867364.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGCDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            23422..24150
                     /gene="recO"
                     /locus_tag="OO349_002316"
     CDS             23422..24150
                     /gene="recO"
                     /locus_tag="OO349_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HCR5867365.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGDTGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDG
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            24162..24893
                     /gene="pdxJ"
                     /locus_tag="OO349_002317"
     CDS             24162..24893
                     /gene="pdxJ"
                     /locus_tag="OO349_002317"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HCR5867366.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKIRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            24893..25273
                     /locus_tag="OO349_002318"
     CDS             24893..25273
                     /locus_tag="OO349_002318"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HCR5867367.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            25693..25773
                     /gene="shoB"
                     /locus_tag="OO349_002319"
     CDS             25693..25773
                     /gene="shoB"
                     /locus_tag="OO349_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HCR5867368.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(25967..26227)
                     /gene="yfhL"
                     /locus_tag="OO349_002320"
     CDS             complement(25967..26227)
                     /gene="yfhL"
                     /locus_tag="OO349_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HCR5867369.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            26442..27535
                     /locus_tag="OO349_002321"
                     /pseudo
     CDS             26442..27535
                     /locus_tag="OO349_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
BASE COUNT         6794 a         6855 c         7219 g         6667 t
ORIGIN      
        1 taagcgaaag gccatccgtc aggatggcct ttttgcattg gcgcagaaaa aaatgcctga
       61 tgcgacgctg cgcgtcttat actcccacat atgacagatt cagcaacgga tgcggtttcc
      121 ccaacttgcc tacttccata cgtgtcctcc ttaccagaaa tttatcctta agctcctcaa
      181 taaccatttt cctgctaact aaattcatgg tttaggttgc ataatgatac gcaacaaatg
      241 tataacattt tctttaccaa aagaaataaa caaaagcgac caacaaaagc atcggattac
      301 ggcaggagac ataatggcat ggctgaaagt actgtaacgg cagacagcaa actgacaagt
      361 agtgatactc gtcgccgcat ttgggcgatt gtgggggcct cttcaggtaa tctggtcgag
      421 tggttcgatt tctatgtcta ctcgttctgt tcactctact tcgcccacat tttcttccct
      481 tccgggaaca cgacgactca actactacaa acagcaggtg tttttgctgc gggattcctg
      541 atgcgcccaa taggcggttg gctatttggc cgcatagccg ataaacatgg tcgcaaaaaa
      601 tcgatgctgt tatcggtgtg tatgatgtgt ttcggatcgc tggttatcgc ctgcctccca
      661 ggttatgaaa ctataggtac gtgggctccg gcattattgc ttctcgctcg tttatttcag
      721 ggattatccg ttggcggaga atatggcacc agcgccacct atatgagtga agttgccgtt
      781 gaagggcgca aaggttttta cgcatcattt cagtatgtga cgttgatcgg cggacaactg
      841 ctagccctac tggttgtcgt ggttttacaa cacaccatgg aagacgctgc actcagagag
      901 tggggatggc gtattccttt cgcgttagga gctgtgttag ctgttgtggc gttgtggtta
      961 cgtcgtcaat tagatgaaac ttcgcaacaa gaaacgcgcg ctttaaaaga agctggatct
     1021 ctgaaaggat tatggcgcaa tcgccgtgca ttcatcatgg ttctcggttt taccgctgcg
     1081 ggctcccttt gtttctatac cttcaccact tatatgcaga agtatctggt aaatactgcg
     1141 ggaatgcatg ccaacgtggc gagtggcatt atgactgccg cattgtttgt attcatgctt
     1201 attcaaccac tcattggcgc gctgtcggat aagattggtc gccgtacctc aatgttatgt
     1261 ttcggttcgc tggcagccat ttttaccgtt cctattctct cagcattgca aaacgtttcc
     1321 tcgccttatg ccgcttttgg tctggtgatg tgtgccctgc tgatagtgag tttttataca
     1381 tcaatcagtg gaatactgaa ggctgagatg ttcccggcac aggttcgcgc attaggcgtt
     1441 ggtctgtcat atgcggtcgc taatgctata tttggtggtt cggcggagta cgtagcgttg
     1501 tcgctgaaat caataggaat ggaaacagcc ttcttctggt atgtgacctt gatggccgtg
     1561 gtggcgtttc tggtttcttt gatgctacat cgcaaaggga aggggatgcg tctttagtga
     1621 cgggtcagtt gccagacggt atagccggtg cttgcaccgg cgacatccca ggccaaatcc
     1681 ttccagctcc agccgctccc ttcggggcgg ctatcccaaa gctcttttga cgcccccaaa
     1741 ctgacagaga acatcaatcc aaacatggca ctgcgatccc ggctcatccc ctgatgctgt
     1801 gaatattcat ttccggcggc ggaaagtatc gccgaggcga taaagtgttg agctttatcc
     1861 tgcccactcc agctatcgtt atccatatgg ctgcatccag aaagtaacaa tagcgaacag
     1921 acaaaaagaa tacgcaaaac gtggcctcca aaaaacaaac cccgtacagg acggggttgt
     1981 gattacagga tgcggctaat taatcggtcg atgcggatac ggcgcaaacg gcggatgagt
     2041 ttacgaacct tcaccggata atcggcaata ctttgcagat cgcgatagtg cttaacgatg
     2101 gtggtacgct cgcggatcag ctccagttct ttctctcgct gcggcgcaag ctccagttgt
     2161 ggatcgtgga tcaaaatggc gttttccaga tccagacgcc aggcgcgcgg gttcaggtta
     2221 ttaccggtga tcaacatcca cttatcatca acccacatcc ctttcaggtg ataggtgttg
     2281 tcgtcatctt tccataaccg aaccactagc tggtcagtat tgacgtaata ctgcaaacgg
     2341 ctcaggaaac gacgcagatt gatctcatag agataaggca atgcgccaat tatcttgaaa
     2401 ggttcatctt ccggaatgta gaagtcgttc gcggttttat caccaacaat aatttcgacc
     2461 tttttccctt cgcgcagcaa ctggataata ttgcgcacaa ggattgctgg caggttgaag
     2521 tatggcgtac agatggttag tttctgctcg gcacaaggca taagatggaa aatggtcttg
     2581 ttcaacagac tcgatttccc cagccccact agcggcgtta cggaaagctg atcgttgtcg
     2641 gcatcgccct ggaaatgata agcggcatca cgcagctcct ggcggaacag acgaatatcg
     2701 ttcttgattt ccgggctttt tggccgatta acatcatcca gacgattaac gccgcggcca
     2761 ttcataatat tctgtgtaac ccattcaaac ataatgtctg acatcttacg gttacggatc
     2821 agatgataac ggtcgtagcg atatttatcg tgttgatgca ggtaaacatc gttcaggctg
     2881 gcaccgctat aaagtacgct atcgtcgatg ataaagcctt taaagtgcag aacaccaaga
     2941 gcttcacggg tattgattgg aacgccataa accggaacat ctacgcccgg attttcctgc
     3001 gccatgcggc agtaccagtc agcgttagtg ttagatgccg cagcgccaat gcgtccacgt
     3061 tgtgcacgat gccagtcgac cagcacccgc acatccagtt ccggacgctg ccttttagcc
     3121 tcatacaacg cgttcagaat gcctttgcca ccgtcatcct gttcgagata cagggcgaca
     3181 atgcaaatgc gctgcttcgc gctggctatt ttttccagca gcgtctcccg gaagtcggcg
     3241 ggagcgtaaa agaaatcgac atcatcaact gattgagaaa tcttgggtag ttgggcaagg
     3301 tgttgttgat gtttattacg cttaaatttt gacaacatca cagtgcattt cttctctgtt
     3361 cattgaaggg tcctctgtgc aatgcagacg acataagcgg gcaataataa caccagtccc
     3421 gattaagtgg tcaacatttc cagtacctta ctcatgattc ctcgcgccgg gcaagattta
     3481 gcgtaagccc aacgatcccc tcttcgagct ggatatcaac gttaaacccg agcttgcggg
     3541 ctagcgccac catgccacga ttgtttggca tcgtaatacc attcagacgt tgtagtccgt
     3601 gatttcgcgt ataggtaatc aacttttcca ttaagcgtcg acctaagcct aaccctttga
     3661 gatccgagcg aaccagtaca gcaaattcgg catcgatgtt atcaggatcg gaaatcgcac
     3721 gcgtgacgcc gaggatctct tccgtttgat caatacgtcg taccgctaca aacgccattt
     3781 cccgatcgta gtcgatctgt gtcatgttgg ctaaatcttc atgggtaaat tcgttgatct
     3841 cgctaaagta gcggtaataa agatcttctt tggtgactcg cgaaatgaat tgctgaagtt
     3901 gtggctcatc ttctggcaaa atcgggcgga acaagcagcg ttcaccgttt ttcaattcta
     3961 cccattcttc cagctgatgc ggataagggc gcactgccag ccgactctcg ttatcgcctt
     4021 caaacggcga gatatccagc gtgacatcca gcgcggtaaa ttcactgcca gaagccagca
     4081 aaggatgaat atccagacgc tgaatttccg ggcaatcgac aatcaagttg gaaacctgca
     4141 ccagaagctg gctcaagcct gcaacatcca atgggcgtag cgcactgcgc gcacgaatct
     4201 ttttactttt gatcccctga ataaccagat agcgggccag gttcatgttc agcggcggca
     4261 gtgcgacgac ggcttgatct tcaggacgcc actccacacc gccttcaccc agcatgatca
     4321 acggcccgaa aaccggatcg tgctcaacca caacccgcaa ctcctgagcg ccagcacggt
     4381 tagccatact ttgcaccaac aggccgtgga cccgcgcctg tggccaggcc atttttacgc
     4441 gatcgaaaat agcgttcgcc gcttgctgga cttcattggc tgtacgcagg taaagcatga
     4501 cgccctgaac ttccgattta tgtggaatat ccggcgaacg caatttcagc gccaccggat
     4561 aaccaatctg ttcggcaata tgcaccgctt cggtgctatc gctggcaatc caggttggga
     4621 gcgtgttcat gccatacgct tgcaggatgg gctgaacttc atgggtatcg agcgacgtag
     4681 ccccttcggc aatcgcctgt tgcaacagaa gatgcgcttc tgcggtattg gaagtcagat
     4741 tgctgggcaa cgccggcgtt tcgcgtagtt gcttctgatt acgccggtac tccaccatat
     4801 gcataaaagc agtgatggtt ccttccgggg tacggtaggt cggcagcccg gcttcgctga
     4861 ataaacgtcg tgcctcttgc gaggagtgct cgccgcacca gttcgtcagc aaagagacat
     4921 atttgctgcg gggatgatgc tttaccgctt caattaatac ttgcgcgctt tctgttgcgg
     4981 gagcagcggc gctgggcgaa tgaataacca tcagtgcatc aaaatcctgg ctgtggagca
     5041 gaatgtccag cgttttgaca taatgctcgc tgctggcgtc atcgcgcaga tcgagcgggt
     5101 tagagactgc cacatgttct ggcaatgcat cgcgcaattt ctggcaggtt tcttcgctta
     5161 acgttgccag cttgccattg cgtgaccata aggcatccag cgccagcgcg gcaggcgcag
     5221 caccgttgct gataatcatc aaccggtcgc cacgcagcgg gcgcatatgg ctaagggttt
     5281 ccaccgccga aaacagttcg tgggtatcct gtacccgcaa caaaccggca cgctgaatag
     5341 ccgcatccca tgccgggtcc attcctgccg tggtgttgag cagtcgctgt gccgccgggc
     5401 tacgtccgct tttaatcacc agaatcggtt tattacgcga ggcactacgg gccgccgaaa
     5461 caaagcgtcg cgcgtcgctt aactgttcga gatagagcag gatggcgctg gttttactgt
     5521 cgcgcgccag atagtcgagc aattcatcaa cgtcgatatc taggctgtcg ccgagcgcaa
     5581 taaagtagga aaagcccatc tcacgctgtt gcgcccagtc gaggatggtg ttggagacgg
     5641 cagccgattg cgaaataaac gccagcttgc cgcgtttaat cggcacaggc gaaaagctgg
     5701 cattcagacc ttgccaggga gccagtaaac ccagactgtt tggtccaagc aggcgcatgt
     5761 tatggcgcag ggcgcaggcg cggagatctt cgtgttgcga tgccggggcg gaaagaataa
     5821 tgcaggtttt acagcctttc tcgccgagct cttccagaag agcaagatta cggctggcat
     5881 tggtacataa aaccgcaagg tcgggtgtaa agggcaagct ggcaatatcc ggccaggcca
     5941 acacacccaa caccgctttc caggctggcg tcaccgggag taccggtccg ttaaagcctc
     6001 ccgccagcag gttacgcatc atcaggtaac ctgcgcgatt gggtttcatc gacgcgccaa
     6061 ttaccgctat cgattttggt cgcagtagtg cttccagtcc tcgctgactc ataccggtct
     6121 cccgcagaag tgaccgaatg attttaaacg ctttctaact gttctgctgt gatgctaccc
     6181 agatgttgcg tttttcctgc cagatagcgt gttttaaagc gggtaaaatg ctcgcctaac
     6241 cctgctgccg ccccggtatc gccggccata tctaacagtg cgatggctac ctcggcagta
     6301 caatattggc ctgcagcctg ggcttcacgc aggcgatagg cagaaagccg ggaaagatcg
     6361 acggaaatga cgggaagatt atccagatac ggacttttac gaaacatctt gcgagcttcc
     6421 ggccaggtac catcgagcat gataaacagc ggtggcttac cggcaggtgg tgtgaagatc
     6481 acttcccgtt gctcatcagc atacgaggcg ggaaagacca ccattggctg ataatccggg
     6541 ttttgtacca gatccagcaa atcctgcgag ggttcggtac gcgaccattg aaacgcaacg
     6601 gtatcaggca aaatatcagc aatcagacgc ccggtattac tgggcttcat tggctcggtg
     6661 tcgaacatca gcaaacagaa gcgacttttt gcttgtgctg gggtaattgt cgaacagaga
     6721 cataatttct ctggcaaaag acagcgttgg cagcgacgaa cgcgattacc gcgggcaaga
     6781 aaaggacgtg ttgcgcgcgc aatacgctcg gcgcgtaact ggagaacagc gttttcggtc
     6841 ataagagagc gtcgaaaaaa cgccattgtc gcagaggaga aaacggggca caagatgcgc
     6901 cccggtaaga ttaaagagat tcgttcagcc agctatcgaa cggcgctttc ggtactgcgc
     6961 cattaagcat gtcgacaacc tgaccgtttt tgaaaatcat gatcgtcggt atactacgaa
     7021 ttccaaagcg actgctcaat tcacgttcag cttcggtatt cactttcaca aagcgcactt
     7081 taccgctacg ctcttgcgcg acatcttcaa aaattggtgc gaaattacgg caggggccgc
     7141 accacggtgc ccagaagtcg atcaccacag gtagatcatc cttcagcaat ttgtcgagtg
     7201 tttcaccggt cgcattaatc acctctccgt caaacaagtc gtgaccgcag cgtccgcatt
     7261 ttgccgcatc ttcgatccga tcgtcgggaa tgcgattgat ggcctgacaa tgggtacaaa
     7321 cggtattcat aactaacctc gggatgagta aggagactta tcggcaacga cgccggtatg
     7381 tttctaatat gttacatatt atcggggaca ttgttccaga caacaatctg ttttattcaa
     7441 taggtatgat agtcgcaggc tttgctacga aatcatggcg aaccgcgcgg acttcgggta
     7501 atctgcgcgc ttcgcgcagc gctggtggag aaaagcatga acgatgaaat gaaaggtaaa
     7561 agcggcaagg tcaaagtgat gtatgtccgc agtgatgatg attctgataa acgtacccac
     7621 aacccgcgta ccgggaaagg gggcgggcgt ccaggaaaat ctcgtgctga tggtggccgt
     7681 cgccccgccc gcgatgacaa acagagtcag ccccgtgacc gcaagtggga agattcgccg
     7741 tggcgcacgg tttcccgcgc gccgggtgat gagacgccgg aaaaggccga tcacggtggc
     7801 atcagtggta aaagttttat tgatccggaa gtgttgcgtc gtcagcgtgc ggaagaaacc
     7861 cgcgtctacg gcgaaaacgc ctgtcaggca ctgttccaga gccgtccgga agcgattgtt
     7921 cgcgcctggt ttatccagag tgtaacgccg cgttttaaag aagccttgcg ctggatggca
     7981 gcaaaccgca aagcgtacca tgtggtggat gaagcggaat tgacaaaagc gtcaggtacg
     8041 gaacatcacg gcggcgtttg cttcctgatc aaaaagcgta acggtacaac cgtgcagcag
     8101 tgggtaatcc aggcaggcgc gcaggattgc gttctggcac tggaaaacga atctaacccg
     8161 cataacctgg gtggcatgat gcgcagctgc gcgcacttcg gcgtgaaagg tgtcgtggtg
     8221 caggatgcgg cactgctgga gtcgggggcg gctatccgta ccgcagaagg cggcgcagag
     8281 cacgttcagc cgattactgg cgacaacatt gttaacgtgc tggatgattt ccgtcaggca
     8341 ggttacaccg tagtgaccac ttccagcgag cagggtaaac cgctgttcaa aaccagtctg
     8401 ccagcgaaaa tggtactggt gctgggtcag gaatatgaag ggttaccgga tgccgcacgc
     8461 gatccgaacg atctgcgcgt gaagattgac ggtacgggca acgttgccgg gctgaatatc
     8521 tctgtcgcaa ccggcgtttt gcttggtgaa tggtggcgtc agaataaagc ctgataaatc
     8581 agccgggtgg caactctgcc atccggcatt tccccgcaaa tttactcaca ctctgccggt
     8641 aatactggca tccagtcaat cggcgtctcg ccacgttgtt ccagccactg atttgccagc
     8701 acaaaatggt tgcagccaaa gaatccacga tgcgccgaaa gcggcgacgg atgcggtgct
     8761 ttcagtacat gatggcgttg cttatctata atcgcccctt tcttttgcgc atgtgatccc
     8821 cacaacaaaa acaccacgcc ttcgcggtgc tggttaatca ggctgatgac tttatcagta
     8881 aacgtctccc aaccgaggct ggcgtgggaa tgcgcctgac ctgcgcgaac cgtcaacaca
     8941 gtattgagca gcagaacgcc ctgacgcgcc cagctttcga gatagccatg attagggcgg
     9001 gtgaagcccg gaatagtatt ttccagctct ttatacatat tcaataatga cggaggaatg
     9061 gcaatgccgg gacgaacgga aaatgccaga ccatgcgcct gtcccggtcc gtgataagga
     9121 tcctggccga gaatcaccac tttaacgtca cccaactcgg taaagcggaa cgcgttaaag
     9181 acatcttttt gtggtggata gatagtgacg ccggactgcc gctcgctggc gacggtctga
     9241 agggtattaa gaaaataagg ttgttgcttc tcttcagcca gcacgtcatg ccaggttaat
     9301 tcgttagcca tcttgctctc ctgcgaatct tcaatccgcc tagcttaact gcttctttct
     9361 acagaacaaa atcatgctca ttttttagac ttaaacgagt gcaaaaaaat gtaaattggt
     9421 aaaatttcac acccgcataa gttgatgtaa aacaataaaa tccatttacg accacaatca
     9481 atgtgtggtt tttgttgatt taaatcaaag aatcaagggt ggttgaggag tatatataca
     9541 ctcaagcaac aatggtttta ccaattggcc gcgacaggct gaacaaatca aataattttg
     9601 ccggggaggc atcacatgat tacaggtatc cagattacta aagccgctaa cgccgatctg
     9661 ctgaactctt tctggctgct ggacagcgaa aaaggcgaag cacgttgcat cgttgcaaaa
     9721 gcaggttatg cagaagatga agtggttgca gtaagcaaac tgggtgacat tgaataccgt
     9781 gaagttccag tagaagtgaa accagaagtt cgcgttgaag gtggtcaaca cctgaacgtt
     9841 aacgttctgc gtcgtgaaac tctggaagat gcagttaagc atccggaaaa atatccgcag
     9901 ctgaccatcc gtgtatccgg ttatgcagtt cgctttaact ctctgactcc ggaacagcag
     9961 cgcgacgtta tcgctcgtac ctttactgaa agcctgtaag gcttaagtag caagttacta
    10021 agcggaagag ggcgacctct tccgcttttt ttcgttaata gaaaatgcgt accgcgcaat
    10081 agaccagcaa cagggcaagc acgatattta actggcgacc atactggcga aacaatcgct
    10141 gaaacagatg ccccgccagc gcccagcaca cattgccaaa cgtcccaatc atcgccagca
    10201 aaacgctgac gccaactacc cagcttagcg cctgtgtttg cggcagaaca aacgtcgaca
    10261 gtgccgtaac accgtacaaa atgattttga cgttcacaaa ctgcaaagca aagctggccc
    10321 aaaagctgat tggttttgcc tgaagtccgt cttcctttgt tgggctggtg gcgattttcc
    10381 acgccagcca gacaatatat gccgcccccg cccaactcaa aaggtgtacc gctgccgggt
    10441 caatcactgc cagtgaaaat gaaatgcccg cacacagtaa catcacaatc aaaaatccca
    10501 gactcatccc tgccagcacg cgggtacttt gacgaaatcc atgcgacgta gcagagctaa
    10561 gggcgagaat attgttcggt cttggcgtca tagcggtaat cagggtgtaa gtccaaaaag
    10621 cacttaaaag gatcggtgtc acttgttatc tcctctgttt tggtagacag tacccgacac
    10681 ggaacattta aaaaaatgaa tgtttttata ttctaaattc gatattttcg ataggtttgg
    10741 ggttatggat ctgcgccgtt ttattacgct taaaaccgtg gtggaagagg gttccttttt
    10801 gcgagcttcg caaaaattgt gctgtacaca atcgacggtg acttttcata ttcagcagct
    10861 tgagcaggag ttttcagtcc agttatttga gaaaattggc cgacggatgt gccttacccg
    10921 cgaaggaaaa aagttgctgc cgcacattta tgaacttacc cgagtgatgg atacgcttcg
    10981 tgaggctgcc aaaaaggagt cagatccgga cggggaacta cgcgttgttt cgggcgaaac
    11041 gttgctctct taccgaatgc cgcaggtgtt gcagcgtttt cggcaacgcg caccaaaagt
    11101 gcgtttatcg ttgcaggcgc tgaactgtta cgtgatccgt gatgcgctac tgaatgatga
    11161 ggccgatgtc ggcgtctttt atcgcgtagg gaatgatgat gccctgaacc gacgagagtt
    11221 gggtgaacaa tcgctggtgc tggtggcttc accacaaatt gcagatgtcg attttactga
    11281 gccgggaaga cataacgcct gtagctttat tatcaacgaa ccgcaatgtg tcttccggca
    11341 gatatttgag agcacgctgc gacagcggcg gatcacggtg gaaaacacca ttgagcttat
    11401 aagtatcgaa agcatcaagc gttgtgtcgc ggcgaatatc ggtgtcagtt acttgcccca
    11461 ctttgcggtg gaaaaagaga ttgagagcgg agaattaatt gaattgcctt ttggcgaaca
    11521 gtcgcagacc attacggcaa tgtgcgcaca ttatgctgga aaagcggtta gcccggcgat
    11581 gcacattttt attcagtgtg ttgaagagtg ttttgtggtt gcgtaaaaaa acaatgccgg
    11641 gcttaaaccc ggcattgaga ttactcttct gtcgtttgcg gtggcacgcc cgttccgctc
    11701 ggtttacggc gcttaccaat atttttggtg tcgcgatggc gttttttcac ccgcggcttc
    11761 tctttctctt tagctttttt cttctcggcg cgttttgcca gtactttctt cgatggcttg
    11821 ccggtctgct tctcgcttgg cgcacgcgtt ttcgggcgta actcatcaat aacgcgagct
    11881 ttaatcggct cttcaatgta acggccaacc ttgcccagca gcaaatggtc atgggcttcc
    11941 accagcgaaa ttgcggtgcc tttacgaccg gcgcgcgcgg tacgtccgat acgatgcaaa
    12001 taagtatcgc cactgcgcgg catatcgaag ttaaagacgt ggctgacgtc aggaatgtcg
    12061 ataccgcgcg cggcaacatc ggtggcaacc agtacgttta cgcggccttc ggtcaggcgt
    12121 ttaattgctt cgttacgctt gccttgtacc atctcacctt cgagatagca gttgttgatg
    12181 cccgcttcgc gcagccagtt tgccagctca tgcacacgtt cacgcttacg cacaaacacg
    12241 attgagcgag tcgcttcagg ctgttttaac aggtgcacca gcaatgcggt tttatgttcc
    12301 aggtcatcgg cgcggtaata ccactgatga atttttttgc gctcacgggt ggacggattg
    12361 gcagaaactt ccaccggatc ttccagcaga cgctcggcaa agtcctgaat cgcatcgcct
    12421 tccagcgttg ccgaaaagag cagggtctgt ttacgccagc gagtttcgcc agcaatatgt
    12481 tcgatatcct gagcgaagcc catatccagc atacggtctg cttcgtcgag gatcagcgtt
    12541 tcaaccgcgc ggcaatcgaa gttctcttct tttatgtatt gcagcagacg tccggtcgtg
    12601 gcgaccacga tgtcctgatt ttcgctgaac acttccgcgt ggttcatata ggctacgccg
    12661 ccggtgatgg tggcgatatc cagatgcgta tgtttcgcca gttcgcgggc atgatcggcc
    12721 acctgcatcg ccagctcgcg agttggggtg aggatcaaaa tacgcggcgg accggatttc
    12781 ttacgtggga aatcgagcag gtgctgcaac gctggcagca gatacgccgc cgttttaccg
    12841 gtgcctgtcg gcgcagaacc gagtacatca cggccatcga gcgcaggcgg aatggcggca
    12901 gcctgaatgg cggtcgggcg agtgaaacct ttatcctgga gggcttccag caggctttcg
    12961 tcgagttcaa gttcggaaaa agtcgttaca gtcatgttct acctctgtgt ggggcgctga
    13021 ttatagacgt tacggctgca atcttcatct gtttgtatgg atatcgcttc tcgggtattg
    13081 ttctcacccg gtgctatcgc cgtttttcct gcaaggtttt attttcatgt cacagtctac
    13141 atccgtgctt cgtcgtaatg gatttacttt taaacagttt tttgttgctc acgatcgctg
    13201 tgcgatgaaa gtgggaacgg atggcatttt gctgggcgca tgggcaccgg tggctggggt
    13261 gaaacgttgc cttgatatcg gcgcgggtag cgggttgctg gcactgatgc tggcgcagcg
    13321 aaccagtgac agcgtgataa ttgatgccgt tgagctggaa agtgaagctg ccgcgcaggc
    13381 gcaggaaaat atcaaccagt ccccgtgggc agagcggatt aacgtccata cggcggatat
    13441 tcagcagtgg atcacacagc agacagtacg cttcgattta atcatcagta acccacctta
    13501 ctatcagcag ggagtagagt gcgcgacacc tcaacgggaa caggctcgct ataccactac
    13561 gcttgatcac ccatcgctgc tgacctgtgc ggcggagtgc attaccgaag agggattttt
    13621 ctgcgtggtg ctgccagagc agattggtaa tggttttact gaactggcat taagcatggg
    13681 ctggcattta cgtttgcgga cggatgtggc ggaaaacgaa gcgcgactgc cgcatcgggt
    13741 gctgctggca ttctccccgc aggcgggaga atgctttagc gatcgcttag tcattcgtgg
    13801 gccagaccag aactattccg aagcgtatac ggcgctgacc caggcttttt atctgtttat
    13861 gtaatgattg ccgggggaaa ggatcgatgg accggaatgc gtgagcagtt ccgggtaatc
    13921 cagcgtaaaa tgcagccccc ggctttcttt gcgcatcatc gcgcagcgaa caatcaactc
    13981 ggcaacctgt accagattac gcagctccag caaattattt gagacgcgga aatgggcgta
    14041 atattcgtct atttcttgtt ggagcatggt tatccgccgc agggcgcgtt ccaggcgctt
    14101 cgttgtgcgc acaatgccaa cgtaatccca cataaacagc cgtagctcgt gccagttatg
    14161 ctgaattact acccgttcgt cagggttctc aacgcggctt tcatcccacg gcggtaacgt
    14221 actgacgccg tgggcataag gcatacgtct ggtgatatct tctgccgccg accagccata
    14281 gaccagacac tccagcaatg aattcgaggc catgcggtta gcgccgtgta agccggtata
    14341 actcacctcg ccaatggcat acaagccctc gacgtccgta cgcccatgat catcaaccat
    14401 tacaccacct caggtatagt gtgcagcagg cacaatcggt accggttctt gtgtgagatc
    14461 aatccccagc ccgagcaatt tttcataaat catcgggaaa tgctggcgaa taaaatcggc
    14521 gggcttatgg ctgatgtcga ggaacataca atctgcgccg aggcgtttca tttcatggtc
    14581 aatggcgcgg gcgacaatat cgcgcggggc cagttcgccg cgctcatcaa aatcgggcat
    14641 aaaacgcgtg ccatccgggc gcttgagata agcgccttcg ccgcgcagtg cttccgttaa
    14701 caggaaattg cgtgcctgtg ggtgatacag tgcggtaggg tggaactgat taaattcgag
    14761 attggcaacc cgacagcccg cgcgccacgc catagcaatg ccatcgccag aagaaatatc
    14821 cggattggtg gtgtactgat acaccttcga cgcaccgccg gttgccagta ccactgcttt
    14881 tgcgtggcag gtttccaccg tttctttatt tcggttccat acccacgcgc ctacaacccg
    14941 tcgcgtgccc ggcaggccaa ttttgtcaga gacaatcaga tcaactgcgt tgctgcgctc
    15001 cagcacacga atattcggat ggttctgcgc cttgctcacc agcgtggatt gtacttctct
    15061 accggtggcg tcggcggcat gaagaatacg acggtgacta tgtccacctt cacgggtcag
    15121 atggtagctt tcttcgccat tcggttgaac gtgggtatca aacaaaaccc cctggtcaat
    15181 taaccatggc acacaggaac gagcattgct ggcgacaaat tcaactgcat ggtgatcgca
    15241 aataccagcc ccggcaatca atgtgtcttc cacatgcgag tcaatgctgt cagtttcatc
    15301 aaacacggcg gcaataccgc cctgggcata aaatgttgaa ccttccgtta ccgggccttt
    15361 acttagaacg atgacctgat gctggtcagc caggcgtagc gccagtgaaa gtccggctgc
    15421 gccgctacca ataatcaaca cgtcacatga atgttcaggg agagtattca ttttctttgt
    15481 ttaatttact aaacattgtt tggtcagcat agcatcatgt tgtgcgaata aacacctgct
    15541 attttaatat ttgttacagt ggctaaacac gttgacgcag ggcggcgaga aaaagagaag
    15601 ttactggctg gtggaggatt aggtggtgaa ataaaaaggc cgttgggtta ctcttcaggc
    15661 agttaaatgg gcatttctac acagataatg cgatgttcag attctgtaga cttataatga
    15721 tagataatga tccgtctaca gcatgacaaa caaaaacaga tgcgttacgg aactttacaa
    15781 aaacgagaca ctctaaccct ttgcttgctc aaattgcagc taatggagtg gcgtttcgat
    15841 agcgcgtgga aatttggttt ggggagactt tacctcggat gagcgagcag ttaacggacc
    15901 aggtcctggt tgaacgggtc cagaagggag atcagaaagc ctttaactta ctggtagtgc
    15961 gctatcagca taaagtggcg agtctggttt cccgctatgt gccgtcgggt gatgttcccg
    16021 atgtggtaca agaagctttt attaaagcct atcgtgcgct ggattcgttc cggggagata
    16081 gcgcttttta tacatggctg tatcggattg ctgtaaatac agcgaaaaat tacctggttg
    16141 ctcaggggcg tcgtccacct tccagtgatg tggatgccat tgaagctgaa aacttcgaaa
    16201 gtggcggcgc gttgaaagaa atttcgaacc ctgagaactt aatgttgtca gaagaactga
    16261 gacagatagt tttccgaact attgagtccc tcccggaaga tttgcgcatg gcaataacct
    16321 tgcgggagct ggatggcctg agctatgaag agatagccgc tatcatggat tgtccggtag
    16381 gtacggtgcg ttcacgtatc ttccgagcga gggaagctat tgataacaaa gttcaaccgc
    16441 ttatcaggcg ttgacgatag cgggatactg gataagggta ttaggcatgc agaaagaaca
    16501 actttccgct ttaatggatg gcgaaacgct ggatagtgag ctgcttaacg aactggctca
    16561 taacccagaa atgcagaaaa cctgggaaag ctatcactta atccgtgatt caatgcgggg
    16621 tgatactccc gaggtgctcc atttcgatat ctcttcacgc gtgatggccg ccattgaaga
    16681 agagccagta cgtcaatcgg cgacattgat cccggaagcc cagcctgcgc cgcatcaatg
    16741 gcagaaaatg ccattctggc agaaagtacg tccgtgggcg gcacagctta cccaaatggg
    16801 cgtagccgca tgcgtatcgc ttgcagttat cgttggcgtc cagcactata atggacaatc
    16861 tgaaacgtcc cagcagcccg aaacgccggt atttaataca ctgccgatga tgggtaaagc
    16921 cagcccggta agcctgggag taccttctga agcgaccgca aacaatggtc aacagcagca
    16981 ggtacaggag cagcgtcgtc gcattaatgc aatgttgcag gattacgaac tgcaacgccg
    17041 actccactct gaacagcttc agtttgagca ggcgcaaacc cagcaagccg ctgtacaggt
    17101 gccaggaatt caaactttag gaacgcaatc gcagtaatga agcaactttg gtttgccatg
    17161 tcattagtga caggtagcct gttattctct gctaacgcct cggccactcc cgcgtccggg
    17221 gcgttattac agcagatgaa cctggccagt cagtcactga attacgagct gtcattcatc
    17281 agcatcaata aacagggtgt tgagtctctg cgttatcgac atgcacgcct cgataaccgt
    17341 cctcttgcac aattgttgca aatggatggc ccgcgccggg aagtggtaca gcgcggcaat
    17401 gaaatcagct attttgaacc gggacttgaa ccgttcacgc ttaatggcga ttacattgtt
    17461 gattctctgc catcgcttat ctataccgat ttcaaacgcc tttctcctta ctacgacttt
    17521 atctccgtcg ggcgcacgcg tattgctgat cgtctttgcg aagtcattcg cgtggttgcc
    17581 cgagatggta cacgctacag ctacatcgtg tggatggaca ccgaatcgaa attaccgatg
    17641 cgggttgatc tccttgatcg cgatggtgaa acgctggaac aatttcgcgt gattgctttt
    17701 aacgtcaatc aggatatcag cagcagtatg cagacgctgg cgaaggcaaa tttgccgccg
    17761 ttgctttctg ttcctgtagg tgaaaaagct aaattcagct ggacgccaac ctggttgcca
    17821 cagggtttta gcgaagtttc cagtagtcga cgtccgctac cgacgatgga caacatgcct
    17881 atcgaatcac gtctctattc cgacggatta ttcagcttct cggtaaacgt taaccgcgct
    17941 acgccatcga gcaccgatca gatgttgcgc accggacgca gaaccgtcag tacaagcgta
    18001 cgtgataacg ccgaaatcac cattgtcggt gaactgccgc cgcaaacggc gaaacgcatt
    18061 gccgagaata ttaagttcgg ggcagcgcaa tgatcaaaga gtgggctacc gtcgtctcct
    18121 ggcaaaacgg gcaggcgctg gtcagttgtg atgttaaagc ttcatgcagc agctgcgctt
    18181 cacgcgccgg ttgcggtagc cgcgtgttga ataaacttgg cccgcaaacc acgcatacca
    18241 ttgtcgtacc ctgtgatgag ccgttagtgc cggggcaaaa agtggaattg gggatcgccg
    18301 aaggcagcct gcttagctcc gcattactgg tttatatgtc gccgctggtg ggattattcc
    18361 tcatcgcttc gctatttcag ctactctttg cttctgacgt cgcagcatta tgtggtgcgg
    18421 tcctcggcgg tattggcggg ttcctgattg cgcgcggcta ctcgcgtaag tttgctgccc
    18481 gggcagaatg gcaaccgatc atcttaagcg tggcattgcc gcctggcctt gtgcgatttg
    18541 aaacatcttc ggaagacgcg agccagtgat ttcactggct ttgttgcatt ttgcataaca
    18601 aatgcgagga cgtttccaga taccacaatc catacggtat gaacatttcc tcgcctcaat
    18661 gttgtagtgt agaatgcggc gtttctatta atacagacgt taagctcaga acagcgactt
    18721 ctaaaagcct ggttaaccgg gcattaaggc acaataatca tactttatga agaatatacg
    18781 taacttttcg atcatagctc acattgacca cggtaaatcg acgctgtctg accgtattat
    18841 ccagatctgc ggtggcctgt ctgaccgtga aatggaggcg caggttctcg attccatgga
    18901 tcttgagcgt gagcgtggca ttaccatcaa agcgcaaagc gtgacgctgg actacaaagc
    18961 gtctgacggc gaaacctatc agcttaactt tatcgacacc ccgggccacg tagacttctc
    19021 ctatgaagtt tcccgttcgc tggctgcctg tgaaggtgca ttgctggtgg tcgacgccgg
    19081 gcagggcgta gaagcgcaaa ccctggcaaa ctgctacacc gccatggaaa tggatctcga
    19141 agtggtgccg gtactgaaca agattgacct gccggcagcc gatcctgaac gcgtggcgga
    19201 agaaattgaa gatatcgtcg gcatcgacgc caccgatgca gtgcgctgtt cagcgaaaac
    19261 cggcgttggc gtgcaggacg ttctcgaacg tctggtgcgc gacattccgc cgccggaagg
    19321 cgatccggaa ggcccgttgc aggcactaat tatcgactcc tggttcgaca actacctggg
    19381 cgttgtttca cttatccgta ttaaaaacgg caccctgcgt aagggcgaca aagtgaaagt
    19441 catgagtacc gggcagacct ataacgccga ccgtctgggc atcttcacgc cgaaacaggt
    19501 tgaccgcact gaactgaaat gtggcgaagt aggctggctc gtatgtgcga ttaaagatat
    19561 ccacggcgct ccagtcggcg ataccttaac gctggcgcgt aatccggcag aaaaggcgct
    19621 gcctggcttt aagaaagtca aaccgcaggt atacgccggt ctgttcccgg taagttccga
    19681 cgactatgaa gccttccgtg acgcgctggg taaactcagc ctgaacgatg cctcactgtt
    19741 ctatgagccg gaaagctcca gcgcgctggg ctttggtttc cgctgcggct tccttggcct
    19801 gctgcacatg gagatcatcc aggagcgtct ggaacgtgaa tacgatctgg atctgatcac
    19861 cactgcgccg accgtagtgt atgaagttga aaccacgtca agggaagtta tctacgtcga
    19921 cagcccatcc aagctgcctg cggtaaataa catttacgaa ctgcgcgagc cgattgcaga
    19981 gtgtcacatg ctgctgccgc aggcatatct cggcaacgtt attacgctgt gcgtagaaaa
    20041 acgcggcgtg cagaccaata tggtttacca cggtaatcag gtggcgctga cgtacgagat
    20101 cccgatggcg gaagtggtgc tcgacttctt cgatcgcctg aaatctacct cgcgtggtta
    20161 tgcgtctctg gattacaact tcaaacgctt ccaggcgtcc gacatggtac gtgtagacgt
    20221 attaatcaac ggtgaacgtg ttgatgcgct ggcgttgatc acccaccgtg ataattcgca
    20281 aaaccgaggt cgcgagttgg tggagaagat gaaagatctg atcccacgcc agcagtttga
    20341 tatcgccatt caggcagcga ttggtacgca cattattgcg cgatccaccg tgaaacagct
    20401 gcgtaaaaac gtactggcta aatgttatgg cggcgatatc agccgtaaga aaaagctgct
    20461 gcagaagcag aaagaaggta agaaacgcat gaagcagatc ggtaacgttg agctgccgca
    20521 ggaagcgttc ctcgccattc tgcacgtcgg caaagacaac aaataaccct taggagttgg
    20581 catggcgaat atgtttgccc tgattctggt gattgccaca ctggtgacgg gcattttatg
    20641 gtgcgtggat aaattctttt tcgcacctaa acggcgggaa cgtcaggcag cggcgcaggc
    20701 ggctgccggt gactcactgg ataaagcaac gttgaaaaag gttgcaccga agcctggctg
    20761 gctggaaacc ggagcttctg tttttccggt gctggctatc gtattgattg tacgttcgtt
    20821 tatttatgaa ccgttccaga tcccgtcagg ttcgatgatg ccgactctgt taatcggtga
    20881 ttttattctg gtagagaagt ttgcttatgg cattaaagat cctatctacc agaaaacgct
    20941 gatcgaaacc ggtcatccga aacgcggcga tatcgtggtc tttaaatatc cggaagatcc
    21001 aaagcttgat tacatcaagc gcgcggtggg tttaccgggc gataaagtca cttacgatcc
    21061 ggtctcaaaa gagctgacga ttcaaccggg atgcagttcc ggccaggcgt gtgaaaacgc
    21121 gctgccggtc acctactcaa acgtggaacc gagcgatttc gttcagacct tctcacgccg
    21181 taatggtggg gaagcgacca gcggattctt tgaagtgccg aaaaacgaaa ccaaagaaaa
    21241 tggaattcgt ctttccgagc gtaaagagac actgggtgat gtgacgcacc gaattctgac
    21301 agtgccgatt gcgcaggacc aggtggggat gtattaccag cagccagggc aacaactggc
    21361 aacctggatt gttccgccgg gacaatactt catgatgggc gacaaccgcg acaacagcgc
    21421 ggacagccgt tactggggct ttgtgcctga agcgaatctg gtcggtcggg ccacggctat
    21481 ctggatgagc ttcgataagc aagaaggcga atggccgact ggtgtgcgct taagtcgcat
    21541 tggcggcatc cattaatagc catcttcgtt cacgttgtcg ccgttatggc gacaacgtga
    21601 attatttatg agataaatct cccgtggcta acgacatccc ccgtcgttgt gtatagaata
    21661 ttcccccgaa gtttaaggtt ggcacctcca ggttgccacg gcacacgaaa cagcgttggt
    21721 ccccatatac cgctaaactg aaactgcagc gaagcagtta gcagagccat gtatatcagg
    21781 tctgtttcgt gtgctgaatt gttgacgcat ttatttattg gtatcgcatg aaccccatcg
    21841 taattaatcg gcttcaacgg aagctgggct acacttttaa tcatcaggaa ctgttgcagc
    21901 aggcattaac tcatcgtagt gccagcagca aacataacga gcgtttagaa tttttaggcg
    21961 actctattct gagctacgtt atcgccaatg cgctttatca ccgtttccct cgtgtggatg
    22021 aaggcgatat gagccggatg cgcgccacgc tggtccgtgg caatacgctg gcggaactgg
    22081 cgcgcgaatt tgagttaggc gagtgcttac gtttagggcc aggtgaactt aaaagcggtg
    22141 gatttcgtcg tgagtcaatt ctcgccgaca ccgtcgaagc attaattggt ggcgtattcc
    22201 tcgacagtga tattcaaacc gtcgagaaat taatccttaa ctggtatcaa actcgtctgg
    22261 acgaaattag cccaggcgat aaacaaaaag atccgaaaac gcgcttgcaa gaatatttgc
    22321 agggtcgcca tctgccgctg ccgacttatc tggtagtcca ggtacgtggc gaagcgcacg
    22381 atcaggaatt tactatccac tgccaggtca gcggcctgag tgaaccggtg gttggcacag
    22441 gttcaagccg tcgtaaggct gagcaggctg ccgccgaaca ggcgttgaaa aaactggagc
    22501 tggaatgagc atcgataaaa gttactgcgg atttattgcc atcgtcggac gtccgaacgt
    22561 tggcaaatcc acattgttga acaaactgct ggggcagaaa atctccatca cctcccgcaa
    22621 ggcgcagaca actcgtcacc gcattgtggg gatccatact gaaggcgcgt atcaggcgat
    22681 ctacgtcgat acaccgggcc tgcatatgga agaaaaacgc gccattaacc gcctgatgaa
    22741 caaagcggcg agcagctcta ttggcgatgt tgagctggtg atttttgtcg ttgaaggcac
    22801 ccgctggacg ccggacgacg aaatggtgct caacaaactg cgcgaaggca aagcgccggt
    22861 aatcctcgcg gtgaacaaag tggacaacgt gcaggagaaa gccgatctgc tgccgcacct
    22921 gcagttcctg gcaagccaga tgaacttcct cgatatcgtg ccaatctctg ccgaaaccgg
    22981 gctgaatgtt gacactattg cggcaatcgt gcgtaagcat ctgcctgaag cgactcatca
    23041 cttcccggaa gattacatca ccgatcgctc acagcgtttt atggcgtctg aaatcatccg
    23101 cgaaaaactg atgcgtttcc ttggcgctga actgccgtac tccgtgaccg tggagatcga
    23161 acgtttcgtc tctaacgaac gcggcggttg tgacatcaac ggtttgattc tcgttgagcg
    23221 tgaagggcag aagaagatgg ttattggcaa caaaggggcc aagatcaaaa ccatcgggat
    23281 tgaagcgcgt aaagacatgc aggaaatgtt cgaagcgcct gtccaccttg aactgtgggt
    23341 aaaagtgaaa tcgggttggg ccgacgacga acgcgcactg cgcagtctcg gttatgttga
    23401 cgatctttaa gagtcactcc gatggaaggc tggcagcgcg catttgtcct gcatagtcgc
    23461 ccgtggagcg aaaccagcct gatgctggac gtcttcacag aggaatcggg tcgcgtgcgt
    23521 ctggttgcca aaggcgcacg ctctaaacgc tctactctga aaggtgcatt acagcctttc
    23581 acccctctct tgctacgttt tggcgggcgt ggcgaagtca aaacgctgcg cagtgctgaa
    23641 gctgtctcac tggcgctgcc attaagcggt atcacgcttt acagcggtct gtacatcaac
    23701 gaacttctct cccgcgtact ggaatacgag acgcgcttct ctgaactctt tttcgattac
    23761 ttgcactgca ttcagtctct tgcaggtgac actggtacgc cagaacccgc gctgcgtcgc
    23821 tttgaactgg cactactcgg gcatctgggc tatggcgtca attttaccca ttgcgcgggt
    23881 agcggcgagc cggtagatgg caccatgacg tatcgttatc gcgaagaaaa agggtttatc
    23941 gcaagcgtcg ttatcgacaa taaaacgttc accggaaggc agttaaaagc gttaaacgcg
    24001 cgggaatttc ctgacgcaga cacactacgc gccgcgaaac gctttacccg aatggcgctt
    24061 aagccgtatc ttggcggtaa accattaaag agcagggaac tgttccggca gtttatgcct
    24121 aagcgaacgg tgaaaacaca ttatgaatga tgaggattgt catggctgaa ttactgttag
    24181 gcgtcaacat tgaccatatt gcaacgctgc gtaacgcacg cggtactgct tacccggatc
    24241 cggtgcaggc ggcgtttatt gccgagcagg cgggagcgga cggcattacc gtgcatttac
    24301 gtgaagatcg ccgccacatt actgaccgcg atgtgcgcat cctgcggcag acgctggata
    24361 cccgaatgaa tctggagatg gcagtgaccg aagagatgct ggcgatcgcc gttgagacga
    24421 agccacattt ttgctgcttg gtaccggaaa agcgtcagga agtaacaacc gaaggcggcc
    24481 tggacgttgc agggcagcgt gaaaaaatac gcgatgcctg caaacgtctg gcagatgccg
    24541 ggattcaggt ttctctgttt atcgacgccg atgaagagca gatcaaagct gcggcagagg
    24601 ttggcgcacc gtttatcgag atccacaccg gttgctatgc tgatgccaaa actgacgccg
    24661 aacaggcgca agagctggtg cgtatcgcca aagccgcgac ctttgccgct agtctcggtc
    24721 tgaaagttaa cgccggacac ggtctgacct atcacaacgt gaaagccatt gccgccatcc
    24781 ctgagatgca tgaactgaat atcggtcatg ccattattgg ccgtgcagtg atgaccggac
    24841 tgaaagatgc ggtggcagaa atgaagcgtc tgatgctgga agcgcgtggc taatggcaat
    24901 attaggttta ggcacggata tcgtagagat cgctcgcata gaagcagtga tcgcccgatc
    24961 cggtgagcgc ctggcacgcc gcgtattaag cgataacgaa tgggcaatct ggaaaacgca
    25021 ccaccagccg gtacgttttc tggcgaagcg ttttgctgtg aaagaagccg cagcaaaagc
    25081 gtttggcacc gggatccgca atggtctggc gtttaatcaa tttgaagtat tcaatgatga
    25141 gctcggcaaa ccacggctac ggctatgggg tgaggcatta aaactggcgg aaaagctcgg
    25201 cgttgtaaat atgcatgtaa cgctggcaga tgaacgccac tatgcttgtg ccacggtaat
    25261 tattgaaagt taaaataata ccgttctctt taaaaagtaa aacccccgaa ctggtaagga
    25321 acgggggttt tactttttaa ctttaatttt gtgcagcatg caccctcaac aacagcgatt
    25381 atggatcagt gtacttatct tttacaagca acttgcaatc tttagcataa aaactcgagc
    25441 ctttacgaag aaagcaatat tgatggaaag attaacgtga ccgccaattc gtaagtacat
    25501 taaaattggc ttcgttattg aagattttgc tgtgctttac accatgccac agaattcccc
    25561 cattgaaacg agtggtgtcg tcaaagctct ggtgtggagt gcagcatgca ccctcaataa
    25621 ctcgcacgtt cagttttggg gagatgtaag ggctaatctg aatggctgca ttccttgttt
    25681 aaggaaaaac gaatgactga ttgccgatac ctgattaaac gggtcatcaa aatcatcatt
    25741 gctgttttac agctgatcct tctgttctta taacacaagg aaacgtactt aaggtgcgtc
    25801 cggtgaacca atcggacgcg cctttaataa ctataaataa gtgtcttggc agatactata
    25861 taaattaact tagtgaatga ttatgctaat gtcatcaatt aaataaatat aatggcgtta
    25921 aggcttccca gtaatataat taatactcta cttccagagt agaatattaa attttatccg
    25981 cgtggtgcat cagcacaaat ttatcccaca actgttcttc tgtctcgaca tgcgccggat
    26041 ctttcacaat agtattgggg atcgggcaca ccttctggca ggttggtgtc tcgtagtgcc
    26101 ctacgcattc ggtacactta tcgctgttaa tttcgtagat atgatctccc attgaaatcg
    26161 cctcattcgg gcattcgggt tcacacatat cacaattaat acagcgttta gtaattagta
    26221 aagacatttc aatggattac cgttaaatca ttttaaaatc agtaagttgt atcgagtttg
    26281 tatgctttac tgtcattaac ttactgtatg ttgatccagt gtatttaacc ttgataaact
    26341 cagtccagca acacaaaacc gcaacacatt gcattttgtc ccgtagaaaa gacttgtatg
    26401 tgtgagcttg ttttctgcgc ctacgcagat aaggattgag aatgccgcgc actgtaacac
    26461 ataatccgga tagccccaat aatgacgatg ttttagccgc atctgaaaaa tgggacgcct
    26521 gtaaaccccc ctataccagc gcacacatga aaatctgtgt tgctgccgcc aaaatcatcc
    26581 tcgctgcttc cggcgtggct cgccgttcca aatacgaaaa agagaactat ctccgtatcg
    26641 atttcagcaa agccggtaag gttacatttt acgccgagtt tccaaaaaag atgggcctca
    26701 aaggtaaaaa gctcggcgag tggccggagc tcgttatcca gctggcgcgc gaaaaagcac
    26761 taggtatggc tgacggtgga ctgcgggcag agtccgtaca tgcagcgctg gaaatgtacc
    26821 gggatgaccc aaagccaaag tagcccggca gaagctgagc ccggacagtt tcacaaccta
    26881 cggggtgcgt atcgaccgga ttaaagcaac gttcggcgag cgtgaggtgt tcagcgacgt
    26941 aacatacaat cggctggtgg aagtgctgga cgagtggatc gccactcgct cgaacaataa
    27001 cgccctggag ttgtttgccg agctccgtcg gttctggaag ttctgcgcac ctactctttg
    27061 caacggccgc aatgttgccg ccagtctgcc agatgattat gtttcctccc gcgtacagaa
    27121 acctaccccc acacggcttt ttaccgatat tgaatcaatc gcccgactct ggctcaatgt
    27181 tgctgcctgc acctctgtac accagaagaa tgctgttcgc ttcatgatca tcactggtgt
    27241 tcgtccgatt aatgtccata acctgcgctg ggactacgtt tacgaggagg ctggtgaaat
    27301 tgtttatccg gaaggggtta tcggcatgcg aggggctatg aaaacacaaa aggctttccg
    27361 cctgccgata acgcctgaga tccggcggat tatcgacgag cagaaagcct ggcgtgattc
    27421 agttcctgag tgcaacaggg attatgtatt tttgcagcca cgtgatccaa tgcagccatt
    27481 ttcaaaacga tcactggata agctggtgaa aacatacagc ccggacgggg ctgta
//
LOCUS       DAJTQY010000054        26562 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.054, whole genome shotgun sequence.
ACCESSION   DAJTQY010000054 DAJTQY010000000
VERSION     DAJTQY010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26562
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.054"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            348..641
                     /locus_tag="OO349_002322"
     CDS             348..641
                     /locus_tag="OO349_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HCR5867370.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            713..1372
                     /locus_tag="OO349_002323"
     CDS             713..1372
                     /locus_tag="OO349_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HCR5867371.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEKRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            1464..1910
                     /locus_tag="OO349_002324"
     CDS             1464..1910
                     /locus_tag="OO349_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HCR5867372.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(2117..3017)
                     /gene="hlyE"
                     /locus_tag="OO349_002325"
                     /pseudo
     CDS             complement(2117..3017)
                     /gene="hlyE"
                     /locus_tag="OO349_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
     gene            3390..3809
                     /locus_tag="OO349_002326"
     CDS             3390..3809
                     /locus_tag="OO349_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HCR5867373.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            3809..5077
                     /gene="umuC"
                     /locus_tag="OO349_002327"
     CDS             3809..5077
                     /gene="umuC"
                     /locus_tag="OO349_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HCR5867374.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVMLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(5123..5653)
                     /gene="dsbB"
                     /locus_tag="OO349_002328"
     CDS             complement(5123..5653)
                     /gene="dsbB"
                     /locus_tag="OO349_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HCR5867375.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGMEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(5799..7340)
                     /gene="nhaB"
                     /locus_tag="OO349_002329"
     CDS             complement(5799..7340)
                     /gene="nhaB"
                     /locus_tag="OO349_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HCR5867376.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYNVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHSIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            7561..8280
                     /gene="fadR"
                     /locus_tag="OO349_002330"
     CDS             7561..8280
                     /gene="fadR"
                     /locus_tag="OO349_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HCR5867377.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(8332..9864)
                     /locus_tag="OO349_002331"
     CDS             complement(8332..9864)
                     /locus_tag="OO349_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HCR5867378.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNCLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGCATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            10194..11492
                     /gene="dadA"
                     /locus_tag="OO349_002332"
     CDS             10194..11492
                     /gene="dadA"
                     /locus_tag="OO349_002332"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HCR5867379.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVDPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKFGDEVIKANAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGSTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            11502..12572
                     /gene="dadX"
                     /locus_tag="OO349_002333"
     CDS             11502..12572
                     /gene="dadX"
                     /locus_tag="OO349_002333"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HCR5867380.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQSDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(12740..14154)
                     /locus_tag="OO349_002334"
                     /pseudo
     CDS             complement(12740..14154)
                     /locus_tag="OO349_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243131.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14765..16501)
                     /locus_tag="OO349_002335"
     CDS             complement(14765..16501)
                     /locus_tag="OO349_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HCR5867381.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(16596..17510)
                     /gene="ldcA"
                     /locus_tag="OO349_002336"
     CDS             complement(16596..17510)
                     /gene="ldcA"
                     /locus_tag="OO349_002336"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HCR5867382.1"
                     /translation="MSLFHLIAPSGYSIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINVHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGAQLTISGHPVLKM"
     gene            17610..18221
                     /gene="emtA"
                     /locus_tag="OO349_002337"
     CDS             17610..18221
                     /gene="emtA"
                     /locus_tag="OO349_002337"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HCR5867383.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(18223..18957)
                     /locus_tag="OO349_002338"
     CDS             complement(18223..18957)
                     /locus_tag="OO349_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HCR5867384.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISTSERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            19158..19412
                     /locus_tag="OO349_002339"
     CDS             19158..19412
                     /locus_tag="OO349_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCR5867385.1"
                     /translation="MGIIAWIIFGLIAGIIGKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIDGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            complement(19462..20922)
                     /locus_tag="OO349_002340"
     CDS             complement(19462..20922)
                     /locus_tag="OO349_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HCR5867386.1"
                     /translation="MHASFAASSDLGYLQVSGSQLKQDFLGLPHGVNNDIAGKHGKMI
                     NSSADDKRGIVKLGFTPRENDEYTLTYIKQDGEKDNPPYSGNCGQKSRYWQWPEYDKE
                     SFYYQGATQLNDRFTLKSRLYRDTFENTLMMYNSLADLKNKKGSYSHYSDYSDGAGLQ
                     LAADVRENDLLSFAVNWKDDVHREKGAPHAAYDRYEDRTWSLASEYQWAAADNVDVVA
                     GISYDWRDSVEAKKHEKDGSITHYDDNNQSAFNWQVMGKYHFANEDTLALSYYDRTRF
                     PTLKERYTTSKPAYNQIAIVNPQLKPERARGVDLTWNGAFTRDWGFEVSVYYNRVSDA
                     ILSHNINADTIQNQNSGTVDYSGLDAGIKGKISNILDVGLSYALIHADAKRKDIGKIT
                     DLPTQTMTAWMTLKPWEPLSVTLSEEARSSSYSNSDGSQKAAGFAVTHIRADYTLGHG
                     FSVNASVNNLFDTQYAYSEGFIEEGRNFWAGVEYTF"
     gene            complement(20859..21416)
                     /locus_tag="OO349_002341"
     CDS             complement(20859..21416)
                     /locus_tag="OO349_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005084083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plug domain-containing protein"
                     /protein_id="HCR5867387.1"
                     /translation="MRLKKRYLCTALSLAFTQQAVAAQESDTLTVWSSPVSSTTTTVL
                     DQPTMKALDKQNVAQALSVVPGVVLQKSGSRNEEQVKVRGFDSRQVPVYFDGVPIYVP
                     YDGNLDLARILTNNLGAVEVSKGYSSLLHGPNQMGGAINITTLKKQVWDIARDGAVVR
                     TMPTICMLHLPPAAILGICKSAVAS"
     gene            complement(21442..22296)
                     /locus_tag="OO349_002342"
     CDS             complement(21442..22296)
                     /locus_tag="OO349_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HCR5867388.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQPGYIAFFHRQGG
                     CVSGVSVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAVQNVLEW
                     SCGVSDYLAQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQGWSGAIAQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYAAPADIKV
                     SLQPTASI"
     gene            complement(22296..23105)
                     /locus_tag="OO349_002343"
     CDS             complement(22296..23105)
                     /locus_tag="OO349_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCR5867389.1"
                     /translation="MLIDDIDFADLYLQQLKLAHRTEKTPDHWDQRAEKMAENCASPT
                     DSYLQQLIAKIDLQGAQTLFDMGCGPGTVSLALADKLTTVYGVDYSQGMLNVAARRAA
                     ALKADNVHWIQRAWEEDWSDLPRCDIAVASRSTLVADMRHAMSKLNNQARLRVYTTHL
                     VSTSFVSPAIQRAAGREVIELPNYIFALNVLYQMGIYAHVDFIRGQNCQQDNSTWERF
                     EQNVSWSLGALNDDERERLYHWYQQQDARALAPASRDWALIWWDSVPQEAL"
     gene            complement(23115..23873)
                     /locus_tag="OO349_002344"
     CDS             complement(23115..23873)
                     /locus_tag="OO349_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5867390.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEILLDSLSVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGL
                     APTIGAFSVPGKQERLKATEQLEKLGILHLATRRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHPLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTNKLAALYRVSADQIHHHLSAISR"
     gene            complement(23870..24850)
                     /locus_tag="OO349_002345"
     CDS             complement(23870..24850)
                     /locus_tag="OO349_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCR5867391.1"
                     /translation="MRIVNGCILLLAAISITFAAVSGAYHLDMQQLLALILRQENESV
                     QEQIVFWQIRVPRILAALLLGAALAGAGTTYQGMLRNPLVSPDILGVSAGAGLGACAA
                     ILWGLPIVYIQLYAFCGGLMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISL
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFGLIVCATLITASTVAIAGIIGWIGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLLLLLRGGRQ"
     gene            complement(24850..25872)
                     /locus_tag="OO349_002346"
     CDS             complement(24850..25872)
                     /locus_tag="OO349_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HCR5867392.1"
                     /translation="MPITRRTFAQALASTLLLQSLPSFSQTVNRFASQSLPEAQNITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLPEHIRQLPRLGRLAGRASTLSL
                     EGLMALHPDLVVDCGNTDETLISQARQVSEQTQIPWLLLNGKLAQSAEQLTTLGKTLG
                     EEHRAAEQANLASHFVGEAQAFATSPAANLRFYAARGPRGLETGLQGSLHTEAAELLG
                     LHNVAQIADRHGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWQGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCSLSDADY
                     QKLVAS"
     gene            26221..>26562
                     /locus_tag="OO349_002347"
     CDS             26221..>26562
                     /locus_tag="OO349_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5867393.1"
                     /translation="MLGVIQNLCCCRPDSPLADSKQRNQKLPQQDFEDFKNDVINVIN
                     NNEDIYFLRESNLLNAFIRTCKKRDDNSYGNMIFTLYFDNDIYGNRIIKASLNGGELQ
                     LLSTGCSNDNIL"
BASE COUNT         6684 a         6472 c         6849 g         6557 t
ORIGIN      
        1 tatggccttt gctgaactca acattgacat tcttacccgc agcaaagctc gtggaggtta
       61 gcagtgcaag cagcaataca gccttactga tgctcttgat tttcattggt agtctcctgt
      121 tcagaatgag tacgatgaac tgttataata taacaatccc ttacgggaga attccagtgg
      181 cttttctgcg ttcggtaaaa tcccggcgcg gcattttttg ccggggggat tacttataaa
      241 ggggcatgga ctcaacaaac cgcagaaatc tacagctggc tggcttgtgg cgcgggtttc
      301 attgatgtta gcttatgcca aaacctggca tcttaaagag taaaagtatg ttttgtgtga
      361 tttatcgaag cagcaagcgt gaccagacct atttatatgt cgaaaaaaaa gacgattttt
      421 cgcgtgttcc tgaggaactg atgaaaggtt ttggtcagcc tcagttagcg atgattctgc
      481 cgttggatgg gcggaagaaa ctggtcaatg ctgatattga aaaggttaaa ctggcattaa
      541 ccgagcaagg ttactatttg cagttaccgc caccacccga agatttgctg aagcaacatc
      601 tttccgtcat ggggcagaaa acagacgaca ctaacaaata accgatatcc ggcggtggca
      661 ttatctttgt cggcgcgggt tttcatatcc acgataaggt gaggggaacg ttatgtatca
      721 acatcacaac tggcaaggtg cgctgctgga ttatccggtg agtaaagtag tctgtgttgg
      781 cagtaactat gccaaacata ttaaagagat gggcagcgca gtgcccgaag agccagtgct
      841 gtttattaaa ccagaaacgg cactgtgcga tctgcggcag ccattggcga tcccatccga
      901 tttcggttca gttcatcatg aagtcgaact ggcggtgttg attggcgcga cgctgcgtca
      961 ggcaacggaa gagcatgtcc gcaaagccat tgccggttac ggcgtggcgc ttgatctgac
     1021 attgcgtgat gttcagggaa aaatgaagaa agccgggcag ccgtgggaaa aggctaaagc
     1081 gtttgataac tcttgtccgc tttccgggtt tatccccgcg gcggaattta ccggcgatcc
     1141 acaaaataca acgctgggcc tgagcgtaaa cggcgaaaaa cgccagcaag gtacgactgc
     1201 ggacatgatc cataaaatcg ttccgttgat cgcttatatg agcaagtttt ttaccctcaa
     1261 ggccggtgac gttgtgttga caggcacgcc tgatggcgtc ggcccgttgc aaagcggtga
     1321 tgagctgaca gtcactttcg atgggcattc tttgacaact cgcgttttgt aatacttttt
     1381 gccgccagaa agcggcggca aaacttgcat cgctgtgcca gactggttat aaggtgcgtt
     1441 ttaacgtaat ggcggaacac ctgatgagcg atgtaccttt ctggcaaagt aaaaccctgg
     1501 acgaaatgag cgatgcggaa tgggagtcgt tgtgtgatgg ttgcggtcag tgttgcttgc
     1561 ataaactgat ggatgaagac accgacgaaa tctacttcac taacgtcgcc tgtcgccagc
     1621 tcaatattaa aacctgtcaa tgtcggaact acgaacgtcg tttcgagttt gaacccgact
     1681 gcattaaatt aacccgtgaa aatctgccaa cattcgaatg gctgccaatg acctgcgctt
     1741 atcggttgct ggcggaaggt aaagatttac ctgcgtggca tccgctactt actggttcga
     1801 aagcggcaat gcatggtgaa cgtatctctg tgcgtcatat cgcagtgaaa gaatcagaag
     1861 tgattgactg gcaggatcat atcttaaata aacctgactg ggcgcagtga taaatttaat
     1921 agatgctgat gggggctttc ttaatatacg tctggatgct cgccatttcg tagccctgtt
     1981 tttgaaagcg ctctgtcata atgagagtta cccgatacct tattatcaac aggtaactct
     2041 caatctttaa aaaacgtctg tgtttttaaa tgtgataaac cttatacctt gagtacatgg
     2101 agagaataat cgcttatcag acttcaggta cttcaaagag tgtcttttta ccgtgtcttt
     2161 tctgatactc attacaggtg ttaatcattt ttttggccgc tgcttttagc aaagaaagca
     2221 ttaaatcatc ataatcaaca tagaatctgg ttgtttcagt ttccgttttt atctccccga
     2281 tggcggctat ttcggtggtt aatttcaatt tggcggcatc gatatcttta ttcgcttgtt
     2341 taaccgtgtt agacagggtg gtaaagaaac tctgcacaga ttttaacttg ttcttcaatt
     2401 ctggaatcag tttcccttca actacgcccg cagcaataga ataggaaatg attaaaccaa
     2461 atggaccggc gacgacatcg gctgcggcac cggcatacgc ttccttcctg attttatcta
     2521 cctgtgactg gaaatagctg cttttttctg aaaaatcatt ggttaactgg ctatctaacg
     2581 ccagcagttt cccggaagcg ttgttgaaac tttgtgagct taccagcagg gatttttgcg
     2641 cttcattcag cttcgtgatg ccgtcatcca gtaccttaat gaggggcgga tgctttcttc
     2701 tcattgtact catcaaatag caaaatatac gctgcgagca attgcgtcgc aacaccacac
     2761 cattcataca ctgtttgggt tgcttcaaaa tacttatcct ggctatccat aagtaaggtt
     2821 ttaatatcgc cgactaaaac ggaggctgcc tgtgaatact cctgtttaaa gcgacttaac
     2881 tcttttatgg tttcatcaaa ggtctgccag gggatgacct gatcgagata tttattataa
     2941 agatctaatg ctccatctgc ggtttcgatt gcgtttttaa ctacttctac cgttttatct
     3001 gcaacgattt cagtcataat cattcgcctc tttaaatata taaattgtaa tgaaactcct
     3061 gttttacaac tattaataaa ttttacttca tctaattcat agttagccgg gcgggatgcg
     3121 tcaatgtctt tatttctatt aatatgataa atatcaaaca atgtttaatg tcattatggc
     3181 gaatgcttct attctatttt tttgccgggt gatatttttc atttctgctg gatgagcgtc
     3241 gtcgccagaa ggccacgtga gcacaggata agagaacgaa aaatcagcag tctatgcagc
     3301 gacaaatatt gatagcctga atcagtattg atctgctggc aagaacagac cactgtatat
     3361 aaaaacagta taacttcagg cagattatta tgttgtttat caagcctgcg gatctccgcg
     3421 aaattgtgac ttttccgcta tttagcgatc ttgttcagtg tggctttcct tcaccggcag
     3481 cagattacgt tgaacagcgc atcgatctga atcaactgtt gatccagcat cccagcgcga
     3541 cttacttcgt caaagcaagt ggtgattcta tgattgatgg tggaattagt gacggtgatt
     3601 tactgattgt cgatagcgct attaccgcca gccatggtga tattgtcatc gctgctgttg
     3661 acggcgagtt tacggtgaaa aaattgcaac tacgcccgac ggtacagctt attcccatga
     3721 acagtgcgta ctcgcccatt accatcagta gcgaagatac gctggatgtc tttggtgtgg
     3781 tgatccacgt cgttaaggcg atgcgctgat gtttgccctc tgtgatgtaa acgcgtttta
     3841 tgccagctgt gagacggtgt ttcgccctga tttatggggt aaaccggtgg ttgtgctatc
     3901 gaataatgac ggttgcgtta tcgcccgaaa cgctgaggca aaggcgctgg gcgttaaaat
     3961 gggcgatccc tggttcaaac aaaaagatct gtttcgtcgc tgtggtgtgg tttgctttag
     4021 cagcaattat gagctttacg cagacatgag caatcgggtg atgtcgacgc tggaagaact
     4081 atcgccccgc gtcgagattt acagtattga tgaagcattc tgcgatctga caggtgtgcg
     4141 taattgtcgc gatctgactg attttggcag agaaattcgc gcaacggtgc tacaacgtac
     4201 ccatcttact gttggtgtgg ggatcgccca gaccaaaacg ttggctaagc ttgccaatca
     4261 tgcggcaaaa aaatggcagc ggcagacggg tggggtggtg gatttatcaa atctggaacg
     4321 ccagcgtaaa ttaatgtctg ctctccccgt ggatgacgtc tgggggattg gacggcggat
     4381 cagcaaaaaa ctggacgcga tggggatcaa aaccgttctc gatttggcgg atacagatat
     4441 ccggtttatc cgtaaacatt ttaatgtcat gctcgaaaga acggtgcgtg aactgcgcgg
     4501 cgaaccctgt ttgcaactgg aagagtttgc accgacgaag caggaaatta tctgttcccg
     4561 ctcgtttggt gaacgcatca cggattatac gtcgatgcgg caggccattt gtagttacgc
     4621 tgcccgggcg gcggaaaaac ttcgcagtga gcatcaatat tgtcggttta tctccacatt
     4681 tattaagacg tcaccatttg cgctcaatga accttattac ggcaatagcg cgtcggtaaa
     4741 actgctgacg cccactcagg acagcaggga tatcattaac gctgctacgc gatctctgga
     4801 tgccatctgg caagcgggcc atcgctacca aaaagcgggc gtgatgctgg gggatttctt
     4861 cagtcaggga gtcgcgcagc tcaatttatt cgatgacaac gcaccgcgcc ccgggagtga
     4921 gcaattgatg gcggtaatgg atactctgaa tgctaaagag ggcagaggaa cactctattt
     4981 tgccgggcag gggatccaac aacaatggca gatgaagcga gctatgcttt caccacgtta
     5041 tacaacgcga agttctgatt tactgcgggt caaataaata tagcggcagg aaaaaagcgc
     5101 tcccgcagga gcgccgaatg gattagcgac cgaacagatc acgttttttc gctttaaacg
     5161 gctgggaaat caccaccagc accgcgacaa tcaggtaagc gataaaaata ccgagcagcc
     5221 actgcggcat ttccatacct aaaaaatccc actgacgctc ggcgcaatcg ccagaggcga
     5281 caaacacttg cggcacccat ttatccagcg gcagccattc cgggaaacga accataaaat
     5341 cacaggtggc aaatggcgaa ggatagagct gaagcatggt gtgctcgtaa gttaactgca
     5401 caccgcggaa cgcactatac aaccagataa ccatcgctac ataacgcagc ggagttttcg
     5461 gggcgatcgc gccaatcagc gcagcaccca gaacgccgaa taacgcgcag cgttcataaa
     5521 tacagagcac gcaaggtttc agtaacatca catgctggaa ccacagcgcc gtcagttcca
     5581 gtgccagagc agtaaacgcc atcaacagcc acgcgccccg gccttgtgaa cattggttca
     5641 aaaatcgcaa cataatcatt tccctgcaat atgcatagag tgcgcagttt aaaccaattc
     5701 attcgctgcg ccaccaggag gcagcaaaaa taggacgcaa taggacaatt atcgggcgaa
     5761 aaggcaaacc gggcatttat atgcccggta agttgttatc aaagcgttgc tatccagccc
     5821 atttgcataa accattcggt tacaggggca agcgtaaact cgacgcagag caagccgacg
     5881 agtgtcagta cgagggtgta aggcagggcc atccacacca tgcggccata agagaggcga
     5941 atcaatggcg cgagtgcaga ggtcagcagg aacaggaacg cagcctgacc gttcggcgta
     6001 gcgacagagg gcagattggt accggtatta atggcgaccg ccagcagctc gtattgcttc
     6061 aacgtgatag cgccactttc cattgccgct ttcgcttcgt tgatataaat cgtccccacg
     6121 aagacgttat ccgaaatgga tgacagcaga ccgttgaaaa tatagaacag cgacagctga
     6181 gcatgttcag atgcctgcaa cacaaactga ataatcggcg aaaacagttg ttggtcgata
     6241 atcaccgcga ctaccgagaa aaacaccgtc aacagtgcgg tgaatggcag agattcggtg
     6301 aaggctttac cgatagaatg ttcatcggtg acaccggtca atgatgttgc cagaataatg
     6361 actgacaaac caatcaagcc aacttccgcc agatgcaacg ccagcgcagt caccagccag
     6421 acgccaataa tcgcctggac aatcagacgg attttatcct gacgggtacg ctggtggcgg
     6481 ctttgatcgt caaactgttg caacacttcg cggactttct ccggcagcgt ttcaccgtag
     6541 ccaaaccaac gcagcttctc taccagcagg caggttaaca ggccacaaat cagaaccgga
     6601 acggtcaccg gcgacatgcg caggaagaaa tcgccaaaat gccagccagc cgctttagcg
     6661 atgatcaggt tctgtggttc gcccaccatg gtcattacgc cgcctaatgc ggtgccgaca
     6721 ccggcatgca tcatcaggct acgcagaaag ccacggaact gttccagaac cacgttgtaa
     6781 tgcttgtcga tatgactatc gtcttgcagg tcggtgtctt cggtacggga agaggctacg
     6841 cgatgataaa taccataaaa accgactgca acgctgatca ccaccgccac gacggttaag
     6901 gcatcgagga acgcggagag gaacgctgcc gccacgcaaa aagagagcga cagcagcatt
     6961 ttggagcgaa tgcttaacag caaacgggta aatatgaaca gcaacagctg tttcataaaa
     7021 tagatacccg ccaccataaa catcaataac agcaagactt caagatttgc cgccacctct
     7081 tcacggacgt gttccgcgct ggtcatgccg atgaataccg cttcgatagc caacagacca
     7141 ccggggagta gcgggtagca tttcagggcc atcgccagag tgaaaataaa ttccgctacc
     7201 agcaaccagc ccgcgacgaa agggctgatg aggaaaatta acgggtttac gattaagaaa
     7261 atgatgaggg cgagtttgta ccagtcgggg gactggccca aaaaattgcg ccatagcgcg
     7321 cggccccagg agatctccat gatggtttcc cttaccttac aaataatcaa tgatgttttt
     7381 atgtttaaac gcaaagctta acggtcaggc aggagtgagg caagtcttga tagtcaaggg
     7441 gaaagagatg cggaaaatga agccttgatc cctttttctt ctttttgtct gctatcagcg
     7501 tagttagccc tctggtatga tgagtccaac tttgttttgc tgtgttatgg aaatctcact
     7561 atggtcatta aggcgcaaag cccggcgggt ttcgcggaag agtacattat tgaaagtatc
     7621 tggaataacc gcttccctcc cgggactatt ttgcccgcag aacgtgaact ttcagaatta
     7681 attggcgtaa cgcgtactac gttacgtgaa gtgttacagc gtctggcacg agatggctgg
     7741 ttgaccattc aacatggcaa gccgacgaag gtgaataatt tctgggaaac ttccggttta
     7801 aatatccttg aaacactggc gcgactggat cacgaaagtg tgccgcagct tattgataat
     7861 ttgctgtcgg tgcgtaccaa tatttccact atttttattc gcaccgcgtt tcgtcagcat
     7921 cccgataaag cgcaggaagt gctggctacc gctaatgaag tggccgatca cgccgatgcc
     7981 tttgccgagc tggattacaa catattccgc ggcctggcgt ttgcttccgg caacccgatt
     8041 tacggtctga tccttaacgg gatgaaaggg ctgtatacgc gcattggtcg tcactatttc
     8101 gccaatccgg aagcgcgcag tctggcgctg ggcttctacc acaaactgtc ggcgttgtgc
     8161 agtgaaggcg cgcacgatca ggtgtacgaa acagtgcgtc gctatgggca tgagagtggc
     8221 gagatttggc accggatgca gaaaaatctg ccgggtgatt tagccattca ggggcgataa
     8281 tcccttccgt ttaaagagca accccctcaa acgaggggtt ttttgttgtt tttacagatt
     8341 tcccattctt ggcgggcaac gttccagcaa ctcgatgctg ccgtcttcgt tttgctgttc
     8401 gagcatcaca tcaaatcccc acaggcgatg cacatgcttc aggacttctt tgcgcccccg
     8461 atccagcggt gcgcgattat gtggaatata acgcagcgtc agcgaacggt cgccgcgcaa
     8521 atccacgttc cagatctgaa tattcggctc cagattactt aagttatatt gcgacgataa
     8581 ccggttacgg atctcccgat aaccttcttc attatgaata gcggaaatct ccagataatt
     8641 atgccgatca tcatccagca cggtgaagaa gcggaaatca cgcatcactt tcggtgacag
     8701 aaactggctg ataaaactct catctttgaa atcacgcatc gcgaaatgca gcgtttccag
     8761 ccagtcggaa ccggcgatat ccgggaacca gtatttgtct tcttccgttg gcgactgaca
     8821 aatccgttta atatcctgga acatggcgaa cccgagggca tacgggttga tgccgctgta
     8881 ccacgggctg ttataggggg gctggaagac cacattggtg tggctgtgca aaaactccag
     8941 cataaaacgt tccgttactt tcccttcatc atacagatgg ttaaggatgg tatagtgcca
     9001 gaaggtcgca cagccttcgt tcatcacctg agtctgtttt tgcggataaa aatactggct
     9061 caccttacgc acaatacgca ggatttcacg ctgccatgat tccagcagtg gcgcattttt
     9121 ctccataaaa tagagcaggt tttcttgtgg ttcggacgga tagcggcgcg cttcagcaac
     9181 cgttttctct tcctcgcgct tcggcagggt acgccagagc atattgacct gactttgtag
     9241 atactcttcg cgacttttct gccgggcttt ctcttcttgc agcgagattt tttgcgggcg
     9301 tttgtaccgg tccacgccgt agttcatcag cgcgtggcac gagtccagaa gccgttctac
     9361 ttcatcaacg ccataacgtt cttcgcactc ggtaatatat ttacgggcaa aaatcagata
     9421 atcgacaatc gaactggcgt cggtccaact gcggaataag caattgtttt taaagaaaga
     9481 gttatgtcca tagcaggcgt gtgccatcac cagcgcctgc atagtaatgg tgttctcttc
     9541 catcaagtaa gcgatacacg ggttagagtt aatgacgatt tcataggcca gcccttgctg
     9601 accgtgctta tacagccgtt cagtctcgat aaacttttta ccgaatgacc agtgcggata
     9661 gttaattggc atgccgacgc tggaataggc atccatcatc tgttctgagg ttatcacttc
     9721 aatctggtgc gggtaggtat ccagccggta gagtttcgcc acccggtcta tctctgccag
     9781 ataaacatcc agcaggtcga acgtccagtc gggtccatcg ctcaaacgtg tggtgtcctt
     9841 attcatagaa tcgatcgtcg ccatacacgc acctcattgt tgtcggcgct ctctgtgtgg
     9901 agcacctcat ttcaagcata gaacacctgt taaaaaccgc gtcgccggag aatttttttc
     9961 tttgcgattt cttattatca gagtgccact aatccgcttc tgaacggaat tttatgctgg
    10021 ataaaaaggg cgttcagcag gagatactaa agacgccata ttgccgcaga gtcagggaga
    10081 tgtgagtcag ctcaccataa aaaagccgta tgttgaataa tattttcaac tgagttatca
    10141 agatgtaatt agattattat tcttttactg tatctaccgt tatcggagtg gctatgcgag
    10201 ttgtcatact gggaagtggt gtggtaggcg ttgccagcgc ctggtactta aatcaggcag
    10261 gacatgaggt caccgtcatt gatcgggagc cgggggccgc cctggaaacc agtgccgcga
    10321 atgccgggca aatctccccc ggatatgctg caccgtgggc agcaccaggt gtgcctttaa
    10381 aagcgattaa atggatgttc cagcgccatg cgccgctggc ggttcgtctc gacggtacgc
    10441 agtttcagtt gaaatggatg tggcaaatgt tacgtaactg cgacaccagc cactacatgg
    10501 aaaacaaagg gcggatggtg cgtctggcgg aatacagccg tgattgcctg aaagcattgc
    10561 gcgccgaaac caatattcag tatgaagggc gtcagggtgg gacgctgcaa ctgttccgta
    10621 ccgaacaaca gtatgaaaat gcgacccgcg atatcgccgt gctggaagat gccggcgtac
    10681 cgtatcagct gctggaatcc agccgcctgg cggaagtgga tcccgcgctg gcagaagtag
    10741 cgcacaaact gacgggcggc ctgcagttac ccaatgatga aaccggagac tgccagctgt
    10801 ttacccagaa tctggcgcgg atggcagagc aggcgggggt taaattccgc tttaatacgc
    10861 ccgttgacca actgctttgc gacggcgagc aaatctacgg cgtgaagttt ggcgatgaag
    10921 tgattaaggc caatgcgtat gtgatggcgt ttggttctta ctcgacggcg atgctcaaag
    10981 gcattgttga tattccggtg tatccgctga aaggctactc gctgactatt ccaattgcgc
    11041 aggaagatgg tgcgccagta tccaccattc ttgatgaaac ctacaaaatc gccattaccc
    11101 gtttcgataa ccgcattcgt gttggcggaa tggcggaaat tgttggtttt aacaccgagc
    11161 tgttgcaacc gcgtcgtgaa acgctggaaa tggtggttcg cgatctctat ccacgcggcg
    11221 gtcatgtcga gcaggcgact ttctggacgg gtttgcgccc gatgacgcca gacggtacgc
    11281 cggttgtcgg gagtacacgc tttaaaaatc tgtggctgaa taccggtcac ggcacgctcg
    11341 gctggacgat ggcttgcggt tccggtcagt tgttaagcga tctgctctct ggtcgcacgc
    11401 cagcgatccc atatgaggat ctaagcgtag cgcgctacag ccgtggattt acgccatcac
    11461 gtccgggcca tttacatggc gcacacagct aaggaaacga gatgacccgt ccgatacagg
    11521 ccagcctcga tctgcaggca ttaaaacaga atctgtccat tgtccgccag gccgcgccgc
    11581 acgcgcgcgt ctggtcggtg gtaaaagcga acgcttacgg gcatggtatt gagcgtatct
    11641 ggagcgcact cggggccacc gatggctttg cattacttaa cctggaagag gcaataacgt
    11701 tacgtgagcg cggctggaag gggccgatcc tgatgctgga aggatttttc catgctcagg
    11761 atctggagat ttatgaccag caccgcctga ccacctgcgt acacagcaac tggcagctca
    11821 aagcactgca aaatgcgcgg ctaaaagcac cgttggatat ttatcttaaa gtgaacagtg
    11881 ggatgaatcg gttaggcttc cagtccgatc gcgtgcttac cgtctggcag cagttgcggg
    11941 caatggcgaa tgttggcgaa atgaccctga tgtcgcattt tgctgaagcg gaacatcctg
    12001 atggaatttc cggcgcgatg gcgcgtattg agcaggcggc ggaggggctg gagtgtcggc
    12061 gttcgttgtc caattcggcg gcgactctgt ggcacccgga agcgcatttt gactgggttc
    12121 ggcctggcat tattttgtat ggcgcttcgc cgtccggtca gtggcgtgat atcgccaata
    12181 ccggattacg tccggtgatg acgctaagca gtgagattat tggtgtccag acgctaaaag
    12241 cgggcgagcg tgtgggctac ggcggtcgct atactgcgcg cgatgaacag cgaatcggca
    12301 ttgtcgccgc aggatacgcc gacggttatc cgcgccatgc gcctaccggt actcctgttt
    12361 tagtggacgg cgtgcgcacc atgacggtgg ggaccgtctc gatggatatg ctggcggttg
    12421 atttaacgcc ttgcccgcag gcggggattg gtacgccggt tgagctgtgg ggcaaggaga
    12481 tcaaaattga tgatgtcgcc gccgctgccg gaacggtggg ctatgagttg atgtgcgcgc
    12541 tggcgttacg cgtcccggtt gtgacagtgt aacttgttgt aagccggatc ggaggcagcg
    12601 tcttctggtt gcgaaaaaat catccatccg gctggtcagc aactgtagtt gttaatgtga
    12661 cagagccatt gcccatgata gtgtccatta aaagaatgga cactatttcc ccggaacctg
    12721 aactcgccgc acaggcgttc tacataaaac gcttacgctt cattgttgac tcggagtcga
    12781 cttcagataa atcacgcttc acccttgatg cagattcggg ttcatgttca gattgttgat
    12841 taacaggctg cgagtcctgt tgtctttcca gactcaacag gcccaactgt tgatccagga
    12901 gctccaggtc ctcctgtacc ttcacgcgct ccaggagttc cgggtcatcc aggaactcca
    12961 gatccataag gtcctccagc tcctcgagtc cttccaggtc aacctagcct tccttgtgca
    13021 aaaagtccaa aaggtccttc aggccctgct gttttttcag gccctcgacg gctttgagca
    13081 gcgtcggtaa attgtctttg tcccacgggt taccattaca gtcaaacacc ttcaggctct
    13141 ggggctgcgg cacctgtacc gttttaaact tgttttctcc acagtagtaa attattagtt
    13201 tgggcggcag ccgtggtgaa gagatcaaat cgttactaga acagtcaaat actttcaggc
    13261 gagtcaagtt ctccagcatc tcatcaggta ttgagttaat attgttgccg gcacaggata
    13321 gtttatacag gtactggggc atcgacggcg gtaaaaattg caagaagttt tcagaacagt
    13381 taatcactct caggttagtc gacagctcag gcagccagat aatactgttt tcatagcact
    13441 tcagtaccat cagttttgag ggctgcacct tcggtaacaa gatcaagacg ttatctgtac
    13501 agtcaagcac cagcagccac tttggcatca ccttcggtaa cgcggtcaac acgtttttac
    13561 tgcagtaaaa ttcaatcaga tgtggaggca aattctcagg gagtgaagtc aatcccaggt
    13621 tgctaagatt gagcatactc cgtcttgtaa tgagacattc cttcattctg cgcaatgctt
    13681 ctgcccggtt ttcaatccgg cttgctggca ggttttcttc caggatattt tccttgattt
    13741 cttccagact ttctttcagg tcttcttggg gaatttctga ctgtttgttt tgctggtttt
    13801 cttccaggat ttcgtacagg tttttttgca ggttttcttg caaaccatcc tgctgtatag
    13861 cccattcgca ccatttggcg accatcatga actcgttatg ttgctgattc agacgagtcc
    13921 ccaacgtgat gatggtattg ttgtccacct gtatgttcac tttgcccgcg atactgaggt
    13981 caatgatctg ccagggggca tcaccccagc tgaatttttg cgggctgaga tacttcatgt
    14041 tctgcaaaat ttgattcttg agaagagccg ccctggcggg cgtcgtacgc ctgggttaac
    14101 ggaagatggg ggaatggcgg gggcgaatgg gcagatgtct ttaaagctag ccataatgct
    14161 acgtttcctt gaaataaata tcctctggca gcgctggaga cgtgaatgtg gtcaccgcaa
    14221 aggagccaca ggacgtcatt tgcgctgata tcactactca tcgttgactt tttccctggt
    14281 cagaaatgcc gtgtcgggtc tggtacatgc tgtattacgg cctaggtagc tctgacgtat
    14341 taaagaaaat acgagagtga acgacccgga aataatcgtt attaagcaaa ggaataacta
    14401 taagaaatcg ctcatgcatc cggtgctaca ggcggatgaa acgcctattg tgtgggctat
    14461 gtcagtgcgg caggttgcgt gggggaatat gttcaggcga tgtcaggcag gagcccctga
    14521 tggttgaccg gtaatgcggg gctggttttt attacctgtt acagtcggaa aaagccctta
    14581 cgctgacttt cttgagccgg tgttttgagt tcattagcca gagcctgcaa tggtttgtca
    14641 tccataattt aaccttcttc tgatgttggg atgagcatat caaaatcagg aaatgacaag
    14701 tttattacag gctcaggctg cgttcagtaa agcatcgcat ccggcgatag atgctgcatt
    14761 aaatttaaga ttcagcttcc tcttcagcca cccgaacgcc aatcttcaac acttcattat
    14821 cttctttctc ggccaccgtc cagatcatcc cggcaaactc tacctggtca ccgacaaccg
    14881 gtgccgcgcc tagcaactgc tggacaattt cacccagcgt ttgctgttta tcacgatatt
    14941 ctcgcccgtc ttccaggcca tatatcagcg caacatcagc atatttggcg ctggcttcga
    15001 gaatgaagtc accaaagaag cgttgatcca gcgcgaccgg tggcgactgg ctgaacagtt
    15061 taccgagcgc cgggagatcg cgttcccgac caattacaca caatacatcg ccttcgcgca
    15121 gtcgggtgct gccggtagga tgaagcaact ggttatcacg aaacagggct gcaatacgcg
    15181 tctcttttgg catatgcaaa tcgcgcagtg ccgcacctac acaccattta tcagcactca
    15241 attgataaac aaactgctcc cacggatttt ccggatgaat atccaggcca acgcgtgaca
    15301 ccggacgtcc gacgggcgga accaccactt tggctttttt cgccgcccac gagagtgatg
    15361 ttccctgcaa gagcagtgaa accagaacca caaagaaggc gacattaaag aacagtcgcg
    15421 cattctccag ccccgccatc atcgggaaca cggctaggat gattggcacc gcgccgcgta
    15481 atcccaccca gctgataaac acgcgctcac gcagattgaa accgcggaaa gggagcaatc
    15541 cagcaaatac cgaaagggga cgggcgaaga atatcatcca tgccgacaaa atgagcgccg
    15601 gaatggcaat aggcagcaga tcgcttgggt taaccaatag ccccagcacc aggaacatgg
    15661 cgatttgcgc cagccaggcg aggccgtcga aattttgcag gatgccgtag cggttgcgaa
    15721 tcggccgatt acccagcaga aaaccgcaca gataaacagc cagaataccg ctgccttcca
    15781 gcgccgtggt taaagcaaaa attaaaatac cgccgcttaa tgccagcaat ggatataatc
    15841 cggcaggcag ggcgatgcga ttaatcattt gcagcagtaa ataaccaccg ccaagaccga
    15901 tgacaatccc gaggccaaat tgctgcagaa tatcgaccac gaacatccaa ctgacactgc
    15961 tttcatgctg ctggatcatt gcaatcaggg taatcgtcag aaagaccgcc attggatcat
    16021 tactgccgga ttcaatttcc agcgtcgagc caacacgttc gttaagcccc ttaccaccca
    16081 gcagagaaaa gaccgctgca gcatcggtgg agccgacgat agcgccgatt aataagcctt
    16141 caatcaaatc aagattaaac agccacgccg ccatcatgcc ggttaaacca gaggtgataa
    16201 gtacgcccag cgtcgccagc gacagcgccg gtcctaacgc cacacgaaag gaactggcct
    16261 gagtgcgcat cccgccgtcg agcaaaataa tcgccagcgc caggttactt accatgtagg
    16321 cgaaggggta attatcaaac gggataccgc cgacgccatc gactcccgcc agcatgccga
    16381 tcgccagaaa aataaccaga ataggaatgc caagacggga agaaaatgaa ctaagtaata
    16441 tactgctggt gacaaggatg gatcctaaga tgaaaaggct aattattgtt gtggcatcca
    16501 acggtcggtt actcctgatt acgctgtctc ttatataaac cctaccatat tagcggcaga
    16561 gacagcgttt tacttagccc tgaagcgtga tttttttaca ttttaagaac aggatgaccg
    16621 ctaatcgtca actgagcgcc ttcccgggtg ttattcagaa tggcatgtgc gcccaacggc
    16681 agcgtaaccg tgcgttgttc atgaccaaaa tcgaggccgg taatgagtgg aatcgacagg
    16741 cgggaacgca aaaacgcgta cactgactcc aggttgtaac ctgcgtcata atcattgggc
    16801 gtgctgccgc taaagctacc gagaataatc gccttctgac gtggcaaaat tcccgcatga
    16861 tagagctgca acagcatacg ttcgacgcgg aaagggtgca cgttaatatc ttccagcacc
    16921 agaataccgt tctcaatttt tggcatccac ggtgtaccaa tcagtgaaat cagcatcgca
    16981 agattgcctc cccacaacgt gccttcggtc tgacatgtcg ggccttcccc ttgccattca
    17041 atggtgaagg tttcattgcg taacgccagc cagaaatggt gctcggtaaa ggcgttcagt
    17101 tcatccgcac caaagtttgc taccagcatg gggccgctaa aggtgatgac attgccttgc
    17161 gccagaagac cgcactgaat ggcggtaaaa tcgctatgtc cgcaaatgag taacgggtca
    17221 tgttgttggc gagccaccag tgcctgccag tcaatatctg ccagtaaacg actggcaccg
    17281 taaccgccgc gtacagccag cacgatggtg ttgggggtag tcagtctggc aagggaattg
    17341 agatcttcca gacgctccgt ttccgtaccc gcaaaacgct cacaacgacg ggcaataacc
    17401 tcgacgttat tgacttgatg ccccgcgtcg gtaaggcgct ggataccacg cagcgcggcg
    17461 tgctgtttaa tgctgtaacc cgatggggca attaagtgaa acagagacat ggcaattcct
    17521 tgctgacaac agaaacgaaa tgtatatcat gccgcttagg tgtgccgttg tcacctcaac
    17581 ggcgattcca ggctataagg atagaagaag tgaaattgag atggtttgcc tttttgattg
    17641 tgttattagc gggttgttca tcaaagcatg actatacgaa cccgccgtgg aacgcgaaag
    17701 ttccggtgca acgtgcgatg cagtggatgc caataagcca gaaagccggt gcagcctggg
    17761 gcgtcgatcc acaattgatc acggcgatta tcgctatcga atcgggtggt aatccgaacg
    17821 cggtgagtaa atcgaatgcc attggtttga tgcagttaaa agcttcaacc tccggacgtg
    17881 atgtttatcg ccgtatgggc tggagtggtg agccgacgac cagcgagctg aaaaatccag
    17941 agcgtaatat ttcaatgggg gcggcttacc tgaatattct ggaaaccggc ccgctggcag
    18001 gcattgaaga tccgaaggta ctgcaatatg cgctggtggt gtcatacgct aacggggcag
    18061 gtgcgctgct acggactttc tcgtcagatc ggaaaaaggc gatcagcaaa atcaacgatt
    18121 tggatgctga cgagttcctc gatcacgtag cgcgaaatca tcctgcgccg caggctccgc
    18181 gctatatcta caaacttgag caggcactgg acgcgatgta aatcagtcgc gcactttgtc
    18241 cgctttttcc cgggcttctc gctcgagaga gaaaataatc cgctgtaatt gccgctccac
    18301 cgtcgggctg acgttaagaa aacggaagct cagacgggga gtggtgatgg tttcattctt
    18361 gccatcaatc actttgcgct cgctggtgga gattaactgg gcgtcaaagt gaaaaacacc
    18421 ccattgcccc atgttgactt caatttgagc gaagcgcata ccttcttgta attcggcagg
    18481 ctttgctgtt tccagtaatg cgcccatgcc gcctaacgac aaatcataca agcggaaacg
    18541 taacgtactg ttatccgcca gtttggtctg gcaaaaataa ggcggatgga gtggggcgga
    18601 gatgcggaaa tatcggcgtc gttgtacaaa ccataaggtg ggaggcggta cggtaataaa
    18661 tgccggaagc tgcaagtatt cactctgctg tagttgttca acagtaaact cgactttcgc
    18721 accctgagtt tcggcggtaa tggtaatgtg ctgtgccttt agcacggcga tgttgtcttc
    18781 ggcttgactg ccgaaatcca gcaccagttt atccggggtt attgccagta ttttgctgat
    18841 cagctgcccg ccattccaac tgagacgcaa aggaattgcg gctttgtgca aatcgcgtaa
    18901 cacgcccagg acggctaacg gattttgttt caggaactgc tcatggtaat gactcacgcg
    18961 gaaaaactcc tgactgacaa actgtctttg gtttatcggt catagttaac aaaacttaat
    19021 acaaatgcgt gaatattttt tacatgttgt tcttaaatca gccgacaaac gcccagcatc
    19081 gatcccgccc ttgcctatac ttagagcgtt gacgtaagca tttcttgcga ccgattcatc
    19141 gaaacgaggg cctgaacatg ggaattattg cctggattat ttttggcctg atagccggca
    19201 ttatcggcaa gctaatcatg ccggggcgtg atggtggtgg atttttcctg acctgtattc
    19261 tcgggatagt cggtgcggtg gtcggcggct ggctggcgac catgtttggc attgacggct
    19321 ccatcagcgg ttttaatttg cacagcttcc tggtggcggt ggtgggcgct atcctggtgc
    19381 tgggcgtatt ccgcctcctg cgaagagaat aagattttca taaggcggat agcgatacag
    19441 atgccgctat ccgctttcac atcagaacgt atactcgaca cctgcccaga aattgcgacc
    19501 ttcttcaata aacccttcac tgtaggcata ttgggtatca aacaggttat tgaccgacgc
    19561 attgacgctg aagccatgac ctaaggtgta atcggctcga atgtgggtca ccgcaaaacc
    19621 ggcggctttt tgtgaaccgt cactgttgct gtagctggag gaacgcgcct cttccgacag
    19681 cgttacgctt aacggctccc atggtttgag tgtcatccat gcggtcattg tctgcgttgg
    19741 aagatcggtt atcttgccga tgtctttgcg tttggcgtca gcgtggatca gagcgtagct
    19801 caatcctaca tccagtatat tgctgatttt ccccttaata ccggcatcca gaccgctgta
    19861 atccaccgtg ccgctgttct gattttgaat ggtatcggca ttgatattgt gcgagaggat
    19921 ggcatcactc acccggttat agtaaacgct gacctcaaat ccccagtcgc gcgtgaaggc
    19981 accattccag gttaaatcca ccccgcgagc gcgttccggc ttgagctgcg ggttaacaat
    20041 cgctatctgg ttatacgcag gtttggatgt ggtatagcgt tctttcagcg tcggaaagcg
    20101 tgtgcggtca tagtacgaaa gcgccagagt gtcttcattg gcaaagtggt atttccccat
    20161 cacctgccag ttaaaagctg actgattgtt gtcgtcataa tgggtgatgc tgccatcttt
    20221 ctcgtgtttt ttcgcttcta cgctatcgcg ccagtcatag ctgattcccg ccacgacatc
    20281 gacattatca gcagcagccc attgatattc actggcgagc gaccaggtac gatcttcata
    20341 gcgatcgtaa gcggcgtgcg gcgcaccttt ttcccggtgt acgtcatctt tccagttaac
    20401 ggcaaacgac agcagatcgt tttcacgcac atcggctgcc agttgtaatc cggcaccgtc
    20461 gctgtaatcg gaataatggc tgtagctgcc ttttttattt ttcaaatcag ccagcgagtt
    20521 gtacatcatc agcgtatttt caaaggtgtc gcgatacagc cgacttttca gggtaaaacg
    20581 gtcgtttagt tgggtcgctc cctgataata aaaactttct ttgtcatact ctggccactg
    20641 ccagtagcgt gatttttgac cacaatttcc gctgtatggc gggttatctt tttcaccatc
    20701 ctgcttaatg taagtcagcg tgtattcatc gttttcgcgc ggtgtaaaac cgagcttcac
    20761 aatgccgcgt ttatcatcag ccgatgaatt aatcatcttg ccgtgtttgc ctgcaatatc
    20821 attatttaca ccatgcggca ggccgagaaa atcctgcttt agctggctac cgctgacttg
    20881 caaataccca agatcgctgc tggcggcaaa tgaagcatgc atatcgtagg cattgtcctg
    20941 actacggctc catccctggc gatatcccag acttgcttct tcagggtggt gatattaatg
    21001 gctccgccca tctgattagg cccatgaagc agcgacgaat accctttgga aacttcgact
    21061 gcccccagat tgttggtcag aatccgcgcc agatcgaggt tgccgtcata ggggacataa
    21121 atgggcacac cgtcgaaata gaccggcacc tgccgactat caaagccacg aacttttacc
    21181 tgttcttcgt tgcggctgcc tgacttttgc agcaccacgc cggggacgac acttaatgcc
    21241 tgagcgacat tctgtttatc caaggccttc atggtgggtt gatcgagaac ggtggtcgtc
    21301 gtcgatgata ccggactgga ccatacggtc agcgtgtcgc tctcctgagc cgctacggcc
    21361 tgctgggtaa aggcaagtga taatgctgtg cagagataac gttttttgag tctcatagaa
    21421 taaattccgt ttaacaaaga attaaataga ggctgtgggt tgcagactga ctttaatgtc
    21481 agctggtgcc gcgtaataag gcgcggaggt aataaaaagg cgaatgccgc agtcgaggta
    21541 atttttgagt gtcgtcagat taattccgcc ggtcagagcc agcgtgcagt gaggagccag
    21601 cgacggcgct atttgcgcaa tttcagttgc ctgttgcgga ctaaatttgt cgagctgaag
    21661 tacgtctggt tgtgcgcgta acgcggcgat agcctctttc ggcgtgtcgg cttcaacaac
    21721 aattttcttc tcgggtgcgt ggcgacgtaa ctgagcgatt gcgcctgacc agccctgatt
    21781 gtcatgaaga aaatggcggt ggttggcaaa caataatatg gtttccgcac atccggcacg
    21841 atgaattagt cctccggcag ccagaattgc ctgcgaagcc agtaagcggg tgcccggaat
    21901 tgcttttcgg gtgcaggcga tattaccgtc aggataacgt tcacgaagca atgccagcat
    21961 ttgagcgaga taatcagaaa caccgcaact ccactccagc acattctgga ctgccttcca
    22021 tccctgatga agtgctgcgg cattaccctg cgcacggatt agccgttgac ccgcgttcgc
    22081 ttgtgaaccg tcgctgaccg cgtcatcaat agttaatccc agcgtagtta acatcttaca
    22141 cgcgacagaa acgccgctga cgcagccgcc ctgacggtga aaaaacgcta tatagccagg
    22201 ctggtgtcca atatttaatg ctcgcgtggt caggtcacct ccctggatat cttccagcag
    22261 taacgcatcg atttgtgcct gagaaaggaa aatcatcaca gtgcctcctg cggtacgcta
    22321 tcccaccaga tgagcgccca gtcgcggctg gcaggggcga gggcacgagc atcttgttgt
    22381 tgataccagt gatataaccg ctcacgctca tcgtcgttaa gcgcgccaag actccagctg
    22441 acgttctgct caaaacgttc ccaggtgctg ttatcctgtt ggcaattttg tccacggata
    22501 aaatcaacgt gggcataaat tcccatctga tacaggacgt tgagcgcaaa gatatagttg
    22561 ggtaattcga tcacttcccg accagccgcc cgctgaatgg ctggagagac gaacgaggtg
    22621 ctgaccagat gagttgtata gacgcgaagc cgtgcctgat tattcagttt gctcattgca
    22681 tggcgcatat cagccaccag agtggagcgg gaagccacgg caatatcgca gcgtggcagg
    22741 tcactccagt cctcttccca ggcgcgctgg atccagtgga cattgtcagc cttcagtgcg
    22801 gcggcgcggc gggcggcaac gttcagcatc ccctgactgt aatcaacgcc ataaaccgta
    22861 gtgagtttgt cagccagcgc cagcgatacg gtgcctggtc cgcagcccat atcgaataag
    22921 gtctgagcgc cttgcaggtc aattttagcg atcagttgtt gcaggtagct gtccgttggc
    22981 gaggcgcaat tttccgccat tttctccgcc cgttgatccc agtgatcggg cgttttttct
    23041 gtgcggtgag ccagtttgag ttgctgcaga taaaggtctg caaaatcaat atcatcaatg
    23101 agcatgattg ttccttagcg gctaattgca gagaggtgat gatgaatttg gtcggcagaa
    23161 acccgataca gggccgccag tttgttcgtt gttaactgtt cggtcggtaa gccttgtgtt
    23221 actcgtccat ctggctctac ttggataatg ctgtcggcaa tggcgttggc atggagcgga
    23281 tggtgagtcg acattaacat tgtcatgccg ttatttttga gctgcgccag cgtgtccaga
    23341 agttggatct gatgaccgaa atcgaggctg gaggcgggct catccagtaa caacaagcgc
    23401 ggttgttgga cgagtgcacg ggcgattaaa gccagctgac gttcaccgcc gctaagcgta
    23461 ttccagcgtc gtgtcgccag atgcaaaatg cccagctttt ccagttgctc cgtggctttc
    23521 aggcgttctt gcttaccggg aacggaaaaa gcgcctatgg tgggggccag gcccatcaac
    23581 accatatcca gcacactgaa agcaaaaata ccgtcgtgcg cctgcggcac ccaggcaata
    23641 gcctgtgcgc gctggcgatg gctgagtgtc tgcacagaga ggctatccag taggatctcg
    23701 ccttttaata acggtataac gccaagaatg gagcgcatga gtgtggtttt gccgcaacca
    23761 ttcgcgccaa gcagacaaca aattgttcct tgcgacaggg tgaaggacac gtcgcgtagc
    23821 actggatggc gggagtaacc cagagacgca tgacgaacgg caagcaaagt cactggcgac
    23881 ctccgcgtaa caacaaaagc aggaaaaacg gtgcgccaac aaaagcggtc agaatgccaa
    23941 gtgggatttc tgttgttcca atgctgcgcg ccagcgtatc ggtaagcagg agcagaatag
    24001 caccagtaca cattgccata ggcaacagct gctgatggtt atgaccggtc aacaagcggg
    24061 caatgtgcgg aacgaccaga ccgatccaac caattatgcc cgctattgct accgtgctgg
    24121 cggtgattag tgtggcgcag acaatcaatc caaagcgcag acgcgtcacg ttcagtccca
    24181 gcgaacgggc ttcgtcgtcg gaaagcgtga gtaaattcat gcgccagcgg agaaaaaaca
    24241 ggggcaagga gcctatgaga atgatcggtg cagcatagca gagatcgcgt aaggtcacgg
    24301 tggacagtcc ccccagcaac cagaaggtaa ttgacggtaa ctgggtgtaa ggatcggcca
    24361 gcgttttaat tagcgatatc cccgctccgc acaacgttcc taacgcaatg cctaccagca
    24421 ccagggttaa gatcggatcg tggcgggtca cccgacgcgt aatcaaccag acgcccgcga
    24481 ccaccatcag cccgccgcaa aatgcgtaca gctggatgta aacaatgggt aatccccaca
    24541 ggattgccgc gcaggcacca agtccggcac cggcggagac gcctaaaata tccggtgaaa
    24601 ctaagggatt acgcaacatt ccctgatagg tggttccggc tcctgccagc gctgcgccca
    24661 gtaacagcgc agcaagaata cgcggcacgc ggatttgcca gaagacaatt tgttcctgca
    24721 cagactcatt ttcttgacga agaattagcg ccagcagttg ctgcatatcg agatgataag
    24781 ccccacttac ggcggcaaaa gtgatactga tagctgccag cagcaaaata catccgttta
    24841 cgattctcat tagctcgcca ccaatttttg atagtcggca tcactgagtg aacaatgcca
    24901 gaacagttgg gcgtaatgct gcatgtcact tttaaactgg cgattgatgg cgggatccag
    24961 ccaggcgtga agtctgcgta atcccagaag acggttgatt cccggcgggg catcgagcca
    25021 gccaaagggc aagccactta aaaataagat gcgttgttcc gcaacggctt tcacgccctg
    25081 ccagagagga tcacgacgaa taaaatctgc agtaacggcc tcctgaacca gaataatatc
    25141 cggctgccag cgcaggagat tttccatgga aacctgtgtc aggccgtggc gatcggctat
    25201 ttgcgcgacg ttgtgcaaac ctaataattc cgccgcctcg gtatgcaacg aaccctgtaa
    25261 gcccgtttcc agccctcggg gaccgcgcgc agcataaaag cggaggttag cggcgggtga
    25321 ggtggcgaat gcctgagctt caccaacgaa gtggctggcg agattggctt gttctgcggc
    25381 gcggtgctct tcgcctaacg ttttgccaag cgttgttaac tgttctgctg attgcgccag
    25441 ttttccgtta agcaataacc aggggatttg tgtctgttcg ctaacctgcc gtgcctggga
    25501 gatcaaggtt tcatccgtat tgccgcaatc aacgaccaaa tcgggatgta acgccataag
    25561 cccttccagc gatagtgtgc tggcgcgccc ggcgagtcgt cccagcctgg gtaactggcg
    25621 aatgtgctct ggcaatggaa ttaatgcctg acgggcaaaa tcaaaagagg aaaaaccgac
    25681 cattttttct ggcgcgacag ccagcaagag caaatcggcg ggcgcgcctg cgctgacgat
    25741 gcgtgtaatg ttttgggctt cgggaagaga ttgcgaagca aaacgattaa ctgtttgtga
    25801 aaaggacggt agagactgca ggagcagggt agatgcaagt gcctgcgcaa acgtccgtct
    25861 ggttatgggc atggagaaca tcctgaaaag taaaatcgat atatactttc atatatatcg
    25921 ataaaaatca tgttcaatat caaagtttag ctattgaaaa catgaagatg tgacgacttc
    25981 cgcaagcctg ttgaccctct ctatcattaa aaatgtattt atcaggtggc taatattatt
    26041 taaatcattt aaggctaaat catttaaata tgaatgacat tattgttttt tatcgtccac
    26101 ggttacgcga cgttgaagtt acttgtttta taatgagcga tttttgatat ctcgccgaga
    26161 aaattatcag cattctctcg tttcagtgtc gtgaatatac tgttaacgga ggtgaaaaga
    26221 atgctggggg ttatacaaaa cctatgttgt tgtcgtccgg attcaccgct tgctgatagt
    26281 aagcagcgta accagaagtt accgcaacaa gactttgagg attttaagaa cgacgttatt
    26341 aatgtcatta acaacaatga agatatttat tttcttagag agagtaattt actgaatgcg
    26401 tttattcgga cgtgcaaaaa aagagatgat aacagttacg gaaatatgat atttacgctt
    26461 tattttgata acgatatcta tgggaacaga attatcaaag catcgctgaa cggcggagag
    26521 ttgcagttat taagcacagg gtgtagcaat gataacattc tc
//
LOCUS       DAJTQY010000055        25718 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.055, whole genome shotgun sequence.
ACCESSION   DAJTQY010000055 DAJTQY010000000
VERSION     DAJTQY010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25718
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.055"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(<1..2128)
                     /gene="yccS"
                     /locus_tag="OO349_002348"
     CDS             complement(<1..2128)
                     /gene="yccS"
                     /locus_tag="OO349_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HCR5867394.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQSYQHDPHFERAFTHIDAALERIRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPRNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWKFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNQALAGLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQI"
     gene            complement(2138..2584)
                     /locus_tag="OO349_002349"
     CDS             complement(2138..2584)
                     /locus_tag="OO349_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HCR5867395.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            2707..4761
                     /gene="helD"
                     /locus_tag="OO349_002350"
     CDS             2707..4761
                     /gene="helD"
                     /locus_tag="OO349_002350"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HCR5867396.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSGGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(4793..5251)
                     /gene="mgsA"
                     /locus_tag="OO349_002351"
     CDS             complement(4793..5251)
                     /gene="mgsA"
                     /locus_tag="OO349_002351"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HCR5867397.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(5347..6009)
                     /locus_tag="OO349_002352"
     CDS             complement(5347..6009)
                     /locus_tag="OO349_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HCR5867398.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            6182..6595
                     /locus_tag="OO349_002353"
     CDS             6182..6595
                     /locus_tag="OO349_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HCR5867399.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(6640..6957)
                     /gene="hspQ"
                     /locus_tag="OO349_002354"
     CDS             complement(6640..6957)
                     /gene="hspQ"
                     /locus_tag="OO349_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HCR5867400.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(7015..8205)
                     /gene="rlmI"
                     /locus_tag="OO349_002355"
     CDS             complement(7015..8205)
                     /gene="rlmI"
                     /locus_tag="OO349_002355"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HCR5867401.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            8300..8578
                     /gene="yccX"
                     /locus_tag="OO349_002356"
     CDS             8300..8578
                     /gene="yccX"
                     /locus_tag="OO349_002356"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HCR5867402.1"
                     /translation="MSKVCIIAWIYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(8575..8904)
                     /gene="tusE"
                     /locus_tag="OO349_002357"
     CDS             complement(8575..8904)
                     /gene="tusE"
                     /locus_tag="OO349_002357"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HCR5867403.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(8995..9654)
                     /gene="yccA"
                     /locus_tag="OO349_002358"
     CDS             complement(8995..9654)
                     /gene="yccA"
                     /locus_tag="OO349_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HCR5867404.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMIANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(9861..9948)
                     /locus_tag="OO349_002359"
     tRNA            complement(9861..9948)
                     /locus_tag="OO349_002359"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9912..9914),aa:Ser,seq:tga)
     gene            10374..11492
                     /gene="hyaA"
                     /locus_tag="OO349_002360"
     CDS             10374..11492
                     /gene="hyaA"
                     /locus_tag="OO349_002360"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HCR5867405.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            11489..13282
                     /gene="hyaB"
                     /locus_tag="OO349_002361"
     CDS             11489..13282
                     /gene="hyaB"
                     /locus_tag="OO349_002361"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HCR5867406.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            13301..14008
                     /gene="hyaC"
                     /locus_tag="OO349_002362"
     CDS             13301..14008
                     /gene="hyaC"
                     /locus_tag="OO349_002362"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HCR5867407.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMIFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            14005..14592
                     /locus_tag="OO349_002363"
     CDS             14005..14592
                     /locus_tag="OO349_002363"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HCR5867408.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQSALAQLAAWGSVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            14589..14987
                     /gene="hyaE"
                     /locus_tag="OO349_002364"
     CDS             14589..14987
                     /gene="hyaE"
                     /locus_tag="OO349_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HCR5867409.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLHEFPDYTWQVAIADLEQSEAIGDRFGAFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            14984..15841
                     /locus_tag="OO349_002365"
     CDS             14984..15841
                     /locus_tag="OO349_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HCR5867410.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSKSEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPM"
     gene            15975..17519
                     /gene="appC"
                     /locus_tag="OO349_002366"
     CDS             15975..17519
                     /gene="appC"
                     /locus_tag="OO349_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HCR5867411.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            17531..18667
                     /gene="appB"
                     /locus_tag="OO349_002367"
     CDS             17531..18667
                     /gene="appB"
                     /locus_tag="OO349_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCR5867412.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            18679..18771
                     /gene="cbdX"
                     /locus_tag="OO349_002368"
     CDS             18679..18771
                     /gene="cbdX"
                     /locus_tag="OO349_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HCR5867413.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            18851..20149
                     /gene="appA"
                     /locus_tag="OO349_002369"
     CDS             18851..20149
                     /gene="appA"
                     /locus_tag="OO349_002369"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HCR5867414.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESMVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     AQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILCRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLNREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(20264..22444)
                     /gene="etk"
                     /locus_tag="OO349_002370"
     CDS             complement(20264..22444)
                     /gene="etk"
                     /locus_tag="OO349_002370"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HCR5867415.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDIVEQKYFPIVGRGWARLTKEKLGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVSAVLARAMLR
                     RGVEAPEQLEEHGISVYSTIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSNAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGTILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(22464..22910)
                     /gene="etp"
                     /locus_tag="OO349_002371"
                     /gene_synonym="yccY"
     CDS             complement(22464..22910)
                     /gene="etp"
                     /locus_tag="OO349_002371"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HCR5867416.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            complement(22898..24037)
                     /locus_tag="OO349_002372"
     CDS             complement(22898..24037)
                     /locus_tag="OO349_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HCR5867417.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(24083..25243)
                     /locus_tag="OO349_002373"
                     /pseudo
     CDS             complement(24083..25243)
                     /locus_tag="OO349_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706907.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
     gene            complement(25322..>25718)
                     /locus_tag="OO349_002374"
     CDS             complement(25322..>25718)
                     /locus_tag="OO349_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5867418.1"
                     /translation="NHLHIEQSKTGAKLAIPLTLTIDALNISLADTLQKCREASSSET
                     IIASKHHDPLSPKTVSKYFTKARNASGLSFDGDPPTFHELRSLSARLYRNQIGDKFAQ
                     RLLGHKSDSMAARYRDSRGREWDKIEIDK"
BASE COUNT         6147 a         6552 c         6823 g         6196 t
ORIGIN      
        1 taatctggcg ttgcagacga ccaatctctg gcagtaatgc aatcaataac ccaacttgtt
       61 gtacgaccag aggttctttg ctatctgccc gtggttcaag ttgctgcatc cgctgtttca
      121 ggccagctaa tgcctgattg acgcgttctt catcagcagg ttgatgatgt aacgcgtcat
      181 caacatagca aactgcgtca tcaagaaacg ccagaatttc aggattagtt aactgctccc
      241 ggtgagcacc gagggctgag atatagctgg taaacgtatg gttaaggcac agcaagcgaa
      301 acgcggcttc gcgaatttgc ggggtaacgt tcggttcgct ggacatattt gataccaccg
      361 acgccagttc agcatcacgg ttgtgtgcat cgcggcgggc aatacgatac gccagacggt
      421 tatcacgccc ctgatggtat tgctccagta tggcatcgag atagcgacag ttggcctctg
      481 tggcgcgttc gagcattcgc ggcagattgc gaaacttcca gtcaggccag atgtagctca
      541 ctgccgccca cgcaatggca caaccaatca gcgtatcgat tacgcgaggt aacgctactt
      601 caaaaccttc acccagtaag ttaaaacaca gtagcaccaa aagtgtgatg aacatcgttg
      661 catgagcgta ttgcacgtta cggaaggcaa aaaagagcac gccggtaata accagcagca
      721 ccagctgccc ttccagtgat ggcacaaacc acagcacagg aatgccaatc gcgataccta
      781 ccagcgtacc aataatcctt aacttcaggc ggtggcgcgt ggcgttatag tttggctggc
      841 agacaaacaa acttgtcagc aagatccaat acccgtgatg cattccggtt atttgaatga
      901 tggcgtagcc gaagcacaac accagcgaca ttcttaccgc atgacggaag agggcggatt
      961 ccggcgtgaa gtgacggcta agacgcagcc agatatcact caatccgtgc gggctgtcat
     1021 cagcgagctc attttcgtca ttattacggg gtagtgcctg ggcctgttct gattcaattg
     1081 ttgccagttg ggcatcaatg gcgcgtaaat tgttcagcaa aaatcccagt gttttgagta
     1141 aatcggcggg tgcgccgtta tcgcgtatcc gctccagcgc agcatcaata tgcgtaaaag
     1201 cgcgctcaaa atgcggatca tgttgataag actgacgcaa caaaatacag cgtgacagtt
     1261 gctggcacgc ctggccctgc atcgacatca gccgctgaaa acggaacagc acgtcgctgt
     1321 ggcgaaaatg ttcacgcaat gtttgatact gaatatgaga agagctggca cgctcgtgaa
     1381 tatcctgtgc gacaaagtaa taatgcagcg tgcgacgcgt tccccgttga ccacgatcgc
     1441 cacgtaagcg ggtcagcagc gagagtttcg tctgattcaa tgtcgccatc agctgaccgt
     1501 tggcgagagc caaatcgtac agcggtgcct ggctttcatc ttcaatatca ggatcaaaca
     1561 tgcgcgactt gagctcaaga taacgcgcca gttgttcata gcaacgcgcc aggttgtcct
     1621 gcagcgggcg gaccgggaac agcagatgac caataagtgt caggacgttg taccagacgg
     1681 caccggccag cagatacatc ggctgctgat accagtgctc atacagtgat gttcccaaca
     1741 tagtgtaaat ggcgatcagc aatgcaccga aggcaattgt tgcatagcgt tgacccagac
     1801 cgccgagcaa aatgaagccg ctggtagaga gcgttaagcc aatcgcaaat agccagggcc
     1861 agggaaacag caattctact gaggccgagg cgataaaaaa gcagaacagc gtaatgatga
     1921 ggttacgcaa acgtcccgcc agtcggtcat cgagatcggt cagcgccgct gccaccatcc
     1981 ccagcgttag cggaatcgtc agttttacat cacccagcca ccacggaaac gctgtggttc
     2041 cacaaagcgc aataaaaata cgcgcgtaat acagccaggc gctgttccag gtatagcgtt
     2101 tgagcaaagg acttagcata aaggccatat tagtggatta ttcaaaacga cgacgtgcat
     2161 tggcttcacg cgcagcttgc gctgtttcta ccgataccac gcgacgacca accggccata
     2221 gtgcaatagc ggcaatttta aagttcgcaa tgccgacagg aatgccaatg attgaaatac
     2281 attgtgcgat gcccgttgca atgtgcatca ggcataacca ccagccaaag aaaatcagcc
     2341 agaaaatatt caataccgta ccgccagtat tcagcaacac atttttgcca gccgggttca
     2401 gttcatcgac atgaatagct tcattgccat aaggcaccag agacagttta gtgatctccc
     2461 agcaggatcg tgtcagcggt aaggtaaaaa tcagcacaat actgaccaga gtcgccaaca
     2521 gccagcccag agtggtggca aatccgccaa gcacaaagtt cagaatgttc aaaacggtac
     2581 gcataaaacc tcgctttact gtggttttca gtaatgggcg gcaattgtaa cgtttttttg
     2641 ggctggagca cgttttctct gacggttaca ctgataagaa ataatttcgt gtggatctac
     2701 agagtcatgg aactgaaagc gacaacgctt ggaaaacgtc tggcacagca cccttacgat
     2761 cgggcggtga tcctcaatgc cgggattaaa gtctccggcg atcgccacga ataccttatt
     2821 cctttcaatc aattactggc gattcactgt aagcgcggtc tggtatgggg cgagctggaa
     2881 tttgtactgc cggacgaaaa agtggtgcgt ctgcacggca cagaatgggg cgagacgcag
     2941 cgtttttacc atcatcttga tgctcactgg cggcggtgga gtggcgagat gagcgaaatt
     3001 gcgtctggtg ttttacgcca gcaactggat ttgattgcca cgcgcactgg agaaaataaa
     3061 tggctgacgc gtgagcaaac ctctggcgtt cagcaacaaa tccgccaggc tttgtcggcg
     3121 ttgccgttgc cggttaaccg actggaagaa ttcgataact gccgtgaggc gtggcgtaaa
     3181 tgtcaggctt ggttgaaaga tattgaaagc gctcggttgc agcataacca ggcgtatacc
     3241 gaagccatgc ttaccgagta tgcggatttt ttccgccagg tcgagtcttc accgctgaat
     3301 ccggcgcagg cccgggcagt cgttaatggc gagcattctc tgttagtgct ggccggtgca
     3361 ggaagcggaa aaacgtcggt gctggtggcc cgtgcaggct ggttgctggc gcgtggtgaa
     3421 gcgtcccctg agcaaatttt attgctggcg tttggtcgca aagccgctga agagatggac
     3481 gagcggattc gcgaacggct acataccgaa gacattaccg cacgcacgtt tcatgcgttg
     3541 gcgctgcata ttattcagca gggcagcaaa aaagttccga tagtcagcaa actggaaaat
     3601 gataccgctg cccgtcatga actctttatt gctgagtggc gcaagcaatg cagcgaaaag
     3661 aaagcgcagg cgaagggctg gcggcaatgg ctgacggaag aaatgcagtg gtcagtgcca
     3721 gaaggtaact tctgggatga tgaaaaatta cagcgtcgcc ttgcctctcg cctcgatcgc
     3781 tgggtaagtc tgatgcggat gcacggtggt gcacaggcag aaatgattgc cagtgcaccc
     3841 gaagagattc gcgatctgtt cagtaaacgt atcaagttga tggccccgtt attaaaagcc
     3901 tggaaaggtg cgctgaaggc agaaaacgct gtcgattttt cgggccttat tcatcaggcg
     3961 attgtgattc tggagaaagg ccgctttatc agcccgtgga agcatattct ggttgatgaa
     4021 tttcaggata tctcgccgca gcgcgcagcg ttgttagcgg cattacgcaa gcaaaacagt
     4081 cagacgacgt tgttcgccgt tggtgatgac tggcaggcga tttaccgatt cagcggtgcg
     4141 caaatgtcgc tcaccaccgc tttccatgaa aactttggtg aaggcgatcg ctgtgattta
     4201 gacacgactt accgttttaa cagtcgtatc ggtgaggtgg caaaccggtt tattcagcag
     4261 aacccaggcc agctgaaaaa gccgctaaac agcttaacca atggagacaa aaaagccgtc
     4321 acgttattgg atgagagtca acttgatgct ttgctggata agctctctgg ttatgccaaa
     4381 ccggaagagc gcattctgat cctggcgcgt taccatcaca tgaggcctgc cagcctggaa
     4441 aaagcggcaa cacgctggcc gaagttgcaa atcgacttta tgaccattca tgccagcaaa
     4501 gggcaacagg cggattacgt catcatcgtt ggcttgcagg aggggagtgg tggttttccg
     4561 gctgcggcgc gggagtcgat tatggaagag gcgctactgc caccggttga ggatttcccg
     4621 gacgctgaag aacggcgttt aatgtacgtg gcgctgaccc gggcacgcca tcgggtatgg
     4681 gcactgttta acaaagagaa tccctctccc tttgtggaaa tactgaaaaa tctggatgtg
     4741 ccggtggcga gaaaaccgta agaaacaggt ggcgtttgcc acctgtgcaa tattacttca
     4801 gacggtcagc gagataacgc tgataatcgg ggataagaat atcgaccgcg tcgttgaaat
     4861 gcggcgactg gataatgaag tctgccgttg ccacgttggt ggcgaccgga atgttccata
     4921 ccgtcgccag acgcagcaag gctttcacgt caggatcgtg cggcacggca tttagtggat
     4981 cccagaagaa tatcaataca tcaattttcc cttctgagat caatgcgcca acctgctggt
     5041 caccccccat tgggccactc aacatcgcgt tgacgttcat gccggtcgcg cgggaaatca
     5101 agttaccggt agtgcctgtt gcatacagta cgtgttgttc cagtaacggt tgatgccgtt
     5161 ccacccagct catcagcatt tgtttgcagt gatcgtgtgc caccagcgca atatgtttcc
     5221 gcgcaggtaa agtgcgagtc gtcagttcca taatgtacat ccgtagttaa ctttcctaca
     5281 gattactgta agcacttatc gctgcaagat aaagaccgaa aaagcctgcg cacaggcaca
     5341 aaaatctcag gaagatggtt gtttttccgc ccattgcagg aaagtatttc gcgtttgtgg
     5401 gtcagccagt ttaaaccaat acttcagccg ttgttctgtg agcacctgag actgcggtgg
     5461 aatagcatcc agctcggaaa cacccgaaag cagcgtactg tcatctgcca tcatcgtggc
     5521 aaattgcggt agtgaagcgc gtttcgcgga tttgttatag cgttcaacct cgacctcata
     5581 atcaatcgtt tttgccgttg aggtaatggc gagaatatcc agctttaccg gaatcggcgt
     5641 cgcatcgcca tccaacaatt caaggcgcgg cgcggcatca aaatggttag cttcccgctc
     5701 attctccagg cgaggcaggc gaaaattgac ctggttgatg agctgggtat taaaactgac
     5761 caccagcggt ggggagatat acagccgttc ttcactattg gaaagatgaa tcgtcttctc
     5821 aacgcgaaac actaactgat gcggtccatt gtccagttca atgctgtccg caccgcgcag
     5881 cagagagctg gaaaccttct tgccgtcgag taccagaaga tcgacatcgg ttgaaagccg
     5941 cagcgtggtg gcaaaaacgg ataccggcaa acataatgca atcaaggtgg tcacgatgcc
     6001 ggttttcata ggtagctcct gtcaaaagac cgcccgaata ataatgtgtc aaaatttttc
     6061 tttaatcata cttactaaca tatagacata tttcccgctt ttgctctcat tcattcgtat
     6121 tagctgcatg gttggcatgt cggctttgtc gtacactttg ttaaacagtc tggaggaaat
     6181 aatgaaagaa accgatattg caggcatttt aacgtccact cacaccattg cgctggtggg
     6241 ggcaagcgac aaacccgatc gccccagcta tcgcgtgatg aaatatctac tcgaccaggg
     6301 gtatcacgtc attccggttt cgccaaaagt tgccggcaaa acgctgctgg gccagcaggg
     6361 gtatggcacg ctggcagacg ttccggaaaa ggtcgatatg gtcgatgttt tccgtaattc
     6421 agaagcggcg tggggcgtgg cacaggaagc tattgccatt ggcgcgaaaa ccctctggat
     6481 gcaactgggg gtgattaatg agcaagcggc agtactggca cgggatgccg ggttaaatgt
     6541 ggtgatggat cgctgcccgg ctattgagat ccctcgcctg ggtctggcca aataaaaaat
     6601 ccccggaagg caaaaacctt ccggggattt gttcagggat tagttacgca gacgcggggc
     6661 ctggagttgt ttgcggatgg tctgcgccag ctcatccatg gatggctgtt caggatgttc
     6721 atcctgcaat tcgctactta gctgggcttc agccaggtag gtatgaaccg gtaggccgtt
     6781 atcgtcctcc atcaccacgt gataccacgg agcggcgcga agctcgtcat tcaccgccag
     6841 ctcatcaggc gacggttcag agagcgaata aaccgggtcg atatccacga ccactccgag
     6901 ataacctaac agggaatggc ggacctgctg gccgataccg aatttgctgg caatcatagt
     6961 cacctcccgg gaaatcttta cctttgatat aagggtaaaa ttccacattt caagttacat
     7021 gacgcgacag gcaaaccctt tcagatatag cccttccggg taggtagcga tcaccggatg
     7081 atcggctgcc tgacggaact gctctataaa ttgtacatca cgaccggcat caattgcggc
     7141 atccgcgatg attttctgaa ataaatcgct ggtcatcaga ccggaacagg agaaagtcag
     7201 gagaataccg ccttcattca gcaactgaat cgccagcatg ttgatgtctt tataaccacg
     7261 acacgcgccc atcaactggc ttttattctc aacaaacttc ggcgggtcca tcacgataac
     7321 gtcaaatttt tcaccgcgat cgcgataagt acgcagcaat ttaaagacat catcacggac
     7381 aaactcagcc ttgctcagat ccagtttgtt cagctcaacg ttctgccgtg caatatccag
     7441 cgcttcctgg gaggtatcaa cgctgacaac ctggctgcaa ccgcccatca gtgccgatac
     7501 ggcgaaacca ccggtatagg agaaacagtt cagcacacgt ttattttcaa cgtagcggcg
     7561 ggtagccagg cggctgtctc gctggtccag atagtagccc gttttgtgtc cgtgctgaat
     7621 atccaccagc agcttcatgc cgtgttcttc aatcggcagc agggcaggcg gcaactcgcc
     7681 ggtgacgagg ccctgggtca gctccatccc ttctttttta cgtaccgcaa catcgctgcg
     7741 atcgtaaatc gcacattccg ggtacagcgt ttgcagggca ctaattaatg ccgcgcgctg
     7801 atattctgcg ccagcactca gcagttgcag caccagaaaa ttaccgaaac gatcgatagt
     7861 aatacccggc aggccatcag attctccggc gattaaacga tagctgtcga ggccatcttt
     7921 ttgcgccagc cagtcacgcc atttttgtgc ttgttgcaaa cggcgggaaa aaaaagcaat
     7981 gtcgatagac tcagacgggt caaacgtcca gacgcgcgcc cggatttgcg aagctggcga
     8041 ataagcgccg cgtgctaacc attttccctg atgatcaaca atatcgatgg tttcaccgag
     8101 gctggcttta ccttccatgc gggcaacggc cccggaaaag acccacggat ggcgacgaag
     8161 taatgatttt tcgcgccctt tggctaacac taaacgtaca ctcataattt acttttctgt
     8221 cgatgcaaag aaatgggcgt cattgtccgg agttttccat ggatttgcaa cgcgtccatt
     8281 aaggataagg agagcagcaa tgtcgaaagt ctgcataatt gcctggattt acgggcgagt
     8341 tcagggcgta ggatttcgct acaccacaca gtacgaagcg aaaagactgg gcttaaccgg
     8401 gtacgccaaa aatcttgacg atggcagcgt ggaagtggtt gcctgcggtg aagaagggca
     8461 ggtagaaaaa ttaatgcagt ggttaaagag tggcggcccg cgttctgcgc gtgtagaacg
     8521 ggtgctcagc gagccgcatc atccctcggg ggaattaacc gattttcgta tccgttaaat
     8581 acattttacc ggttttggca ggccagcaat tttggtggct tgcttagccg ggcctttcgg
     8641 gaacagtcgg tataaatagc ggctgttacc tttttcttca ccaaatttat tcgccatcgc
     8701 ttttaccagc atacgaatcg ccggagacgt attaaattcc aggtagaagt cacgtacaaa
     8761 acgcaccact tcccagtgtt ctggcgacag cgaaatccct tcgttctctg caatcaccac
     8821 tgccagcggt tcactccact ggctgctttc tttgagatag ccttcggtat ccgtttctat
     8881 ctctttacct tcgaagatca gcataattat tactacctta atcagactgg cggcagtgta
     8941 acaaaaaaca aagccccgca taaagcgagg ctatgaaagt gttagcgggt gagattaatc
     9001 gcggctggcg aagcccagaa tgctcagcag gctgacgaag atgttgtaca gcgaaacata
     9061 caggctaacc gtggcacgaa tatagttcgt ttcaccgcca tgaatgatgt tgctggtttc
     9121 aaacaagata gcgccagagg agatcagaat gaagaccgcg ctgatcgcca gatgcagagc
     9181 aggcagttgc aggaagatat tagcaatcat accaatcagt accaccacaa tacccgccat
     9241 cagcataccg ccgaggaacg acatatcttt gcgggtggtc agcacatatg cagagcagca
     9301 gaagaacact aacgccgttc cgcccagtgc catagcgatt acgtcaccca ttccggcaga
     9361 cagataggtg ttcagaattg gtccgaggat ataacccaga aaaccggtaa aggcgaatgc
     9421 ggagataatc ccggtcggct tattcgccgt tttataggtc aggaacatca aaccatacat
     9481 acccaccaga gtcagaatca gacccggaga tggcagcatc agcaccgtac tggcagttgc
     9541 ggtaatcgcc gaaaaggcca gcgtcaggct cagcagaaaa taggtattac gcagcacctt
     9601 atgggtgcta agcagtgatg tacggtcatg tgaagaacta acaatacgat ccattgagtc
     9661 actctctatg acagatgtaa ttaattaagc agcataatga taatgcgtaa gggcacccag
     9721 aagttttacc catctttacg catttgatct ggaacaggtt taacagcgga ttatcaggtc
     9781 attaagcaaa tataacgccc tgagaattcc gacaggcaaa agaaaaaggg ggtagcgttt
     9841 agctaacccc ttatcttatt tggcggaagc gcagagattc gaactctgga accctttcgg
     9901 gtcgccggtt ttcaagaccg gtgccttcaa ccgctcggcc acacttccgg aatgacgcgc
     9961 actataaaca tcccgatgcg gcgtgtaaac ccctaatttg tttgtttgcc tgaaaaacag
    10021 ccaaaagtgc attgatagcg tgaaataaca gcagattgat catttcatca ccaggaattc
    10081 cttctctttt actcgtttag caaccggcta aacatcccca tcgcccggct aaagaaaaat
    10141 aggtccattt ttatcgctaa aagataaatc cacacagttt gtattgtttt gtgcaaaagt
    10201 ttcactacgc tttattaaca atactttctg gcgacgtgtg ccagtgcaga aggatgagct
    10261 ttcgttttca gcatctcacg tgaagcgatg gtttgccttg ctacagggac gtcgcttgcc
    10321 gaccataagc gcccggtgtc ctgccggtgt tgctaggaga agagacgtgc gatatgaata
    10381 acgaggaaac attttaccag gccatgcggc gtcagggcgt tacccggcgc agctttctca
    10441 aatattgtag tctggctgcc acgtcgctgg gattaggcgc gggaatggca ccaaagattg
    10501 cctgggcgct ggagaacaaa ccgcgcattc cggtggtatg gatccacggt ctggaatgta
    10561 cctgctgtac cgaatctttt atccgctccg ctcacccact ggcgaaggac gtcatccttt
    10621 ccctgatttc cctcgattac gacgataccc tgatggctgc cgccggaacc caggcggaag
    10681 aagtctttga agacatcatc acgcaatata atggcaaata tatcctcgca gtagaaggta
    10741 atccgccgct gggcgagcag gggatgttct gtatcagcag cggtcgaccg tttattgaaa
    10801 aactcaaacg tgccgctgcc ggagccagcg cgattatcgc ctggggaacc tgcgcgtcct
    10861 ggggctgcgt gcaggccgcg cgacctaatc cgacacaggc aacgcctatc gacaaagtca
    10921 tcaccgacaa acccattatc aaagtacctg gctgcccgcc gatcccggat gtgatgagcg
    10981 ccatcattac ttacatggtg acctttgatc gcttgccaga tgtcgacaga atgggtcgtc
    11041 cactgatgtt ttatggtcag cgtatccacg ataaatgcta tcgccgcgct cacttcgacg
    11101 ccggagagtt cgtccagagt tgggatgatg acgctgcccg caaaggttat tgcctgtaca
    11161 aaatgggctg caaagggcct accacctata acgcctgttc ctccacacgc tggaatgatg
    11221 gcgtttcttt cccaatccag tctggtcacg gctgcctggg ctgtgcggaa aatggtttct
    11281 gggatcgcgg ttcgttctac agccgcgtgg tcgatattcc gcaaatgggt actcattcca
    11341 ccgccgatac cgtcggttta accgcgcttg gcgtggtggc agcagctgtt ggtgtgcacg
    11401 cagttgccag cgccgttgac caacgcagac gtcataacca gcaacctaca gaaaccgaac
    11461 atcagccagg caatgaggat aaacaggcat gagcactcag tacgaaactc agggatacac
    11521 catcaataat gccggacgcc gcctggtggt cgacccgatt acgcgcatcg aaggccacat
    11581 gcgctgtgaa gtgaatatta acgatcagaa tgtgatcacc aatgccgtct cctgcggcac
    11641 catgtttcgc gggctggaga tcatcctgca agggcgcgac ccgcgcgatg cgtgggcgtt
    11701 cgttgaacgt atctgcggcg tctgtactgg cgtacacgcc ctggcttcgg tttacgccat
    11761 cgaagatgcc atcggtatta aagtgccgga caacgccaat atcatccgca acattatgct
    11821 ggcaacgctc tggtgccacg atcatctggt gcacttctat cagcttgccg ggatggactg
    11881 gatcgacgtg ttagatgcgc tgaaagccga cccgcgaaaa acctccgaac tggcgcaaag
    11941 tctctcctct tggccgaaat catcccctgg ctatttcttc gacgtacaaa atcgcctgaa
    12001 gaaattcgtt gaaggcgggc agttggggat cttccgcaat ggctactggg ggcacccgca
    12061 gtacaaactg ccgccagaag ccaacctgat gggctttgcc cactatctcg aagctctcga
    12121 tttccaacgt gaaattgtca aaattcacgc ggtctttggc ggtaaaaacc cgcatccaaa
    12181 ctggattgtc ggtgggatgc cttgcgccat taacattgac gaaagcggcg cggtcggggc
    12241 ggtcaatatg gaacgcctga acctggtgca gtcgattatc acccgcacgg cagacttcat
    12301 taacaacgtg atgatccccg acgccttagc catcggtcag ttcaacaaac cgtggagcga
    12361 aatcggcacg ggtctttctg ataaatgtgt cctcagctac ggcgcattcc cggatattgc
    12421 caacgacttt ggtgagaaaa gtctgctgat gcctggcggc gcggtgatta acggcgactt
    12481 caacaatgtg ctgccagtgg atttggttga tccgcagcag gtgcaggagt ttgtcgatca
    12541 cgcctggtat cgttatccca acgatcaggt cgggcgtcat ccattcgatg gcattaccga
    12601 cccgtggtac aaccccggcg atgtcaaagg cagcgatacc aacattcagc agctgaatga
    12661 acaggaacgc tactcgtgga tcaaagcgcc acgctggcgc ggtaacgcga tggaagtggg
    12721 gccgctggca cgcacgttaa tcgcttatca caaaggcgat gctgcgaccg ttgagtcggt
    12781 cgatcgcatg atgtcggcgt tgaacctgcc gctttccggt atccagtcaa cgttgggccg
    12841 cattttgtgc cgcgcgcacg aagcgcagtg ggccgcaggt aagttgcagt atttcttcga
    12901 caagctgatg accaacctga aaaacggcaa tctcgctact gcttccactg aaaaatggga
    12961 acctgcaacc tggccgacag agtgccgtgg cgtcggcttt accgaagcgc cgcgcggagc
    13021 gttaggccac tgggccgcca ttcgcgatgg caagattgat ctctaccagt gcgtggtgcc
    13081 gaccacctgg aacgccagtc cgcgcgatcc caaagggcag attggcgctt atgaagcggc
    13141 gctgatgaac accaaaatgg cgatccccga gcaaccgctg gagatactgc gtactctgca
    13201 cagctttgat ccgtgcctcg cctgttcaac tcatgtgctg ggcgacgacg gtagcgagct
    13261 gatctccgtg caggtgcgtt aacagcgaag gagaatcatc atgcaacaga aaagcgacaa
    13321 cgttgtcagc cactatgtct ttgaagcacc agttcgtatc tggcactggc tgacggtgtt
    13381 atgcatggcg gtgttgatgg tcaccggtta ctttatcggc aagccgctac cttccgtcag
    13441 tggcgaggcg acgtatctgt tctatatggg ttacatcagg ttaattcact tcagcgccgg
    13501 gatgattttt accgtggtct tgctgatgcg gatctactgg gcttttgttg gcaatcgtta
    13561 ctcccgcgag ctgtttatcg tgccggtatg gcgtaaaagc tggtggcagg gcgtgtggta
    13621 tgaaatccgc tggtatctgt ttctggcaaa acgtccgagt gccgatatag gccataaccc
    13681 catcgcccag gcggcgatgt tcggctattt cctgatgtcg gtctttatga tcatcaccgg
    13741 ttttgcgctg tacagcgaac acagccagta cgctattttt gcgccgttcc gttatgtggt
    13801 ggaatttttc tactggacgg gtggcaactc aatggacatt cacagctggc atcggctggg
    13861 gatgtggctg attggggcgt ttgtgatcgg tcatgtctac atggcgctgc gtgaagacat
    13921 catgtccgac gacacggtga tctccaccat ggtcaacggc taccgtagcc acaaatttgg
    13981 caaaataagt aacaaggagc gttcatgagc gagcaacgcg tggtggtcat ggggctgggc
    14041 aacctgctgt gggccgatga aggcttcggc gtgcgggtgg cggaacggct gtatgcccat
    14101 taccactggc ccgagtatgt ggagattgtc gatggcggta ctcagggact gaacttgctg
    14161 gggtatgtcg aaagcgccag ccatctgttg attctcgatg ccattgacta cgggctggaa
    14221 cctggaacgc tgcgaaccta tgccggagaa cgcattccgg cctatctcag cgcgaagaaa
    14281 atgagcctgc atcagaacag tttctccgaa gtgctggcgc tggcagatat ccgcggacat
    14341 ctgccagcac atattgccct cgtcgggctg caacccgcga tgctcgacga ctacggcggc
    14401 agcctgagcg aactggcacg ggagcaactg cccgctgcgg aacaatcggc gctggcgcag
    14461 ttggctgcgt ggggaagtgt gccgcaaccg gctaatgaat cgcgttgtct caattatgac
    14521 tgtctgtcga tggaaaatta cgaaggcgtt cgcttgcgcc agtaccggat gacacaggag
    14581 gagcagggat gagcaacgac acgccatttg atgcgttgtg gcaacgaatg ctggcgcgcg
    14641 gctggacgcc ggtcagtgaa tcccgtcttg acgactggct tacgcaagcg ccagacggcg
    14701 tggtgttatt aagcagtgac ccgaaacgca cgccagaggt cagcgataat ccggtaatga
    14761 ttggcgaatt actgcacgag tttcccgact atacatggca ggtggcgatt gctgaccttg
    14821 agcagagcga agccatcggc gatcgctttg gcgcctttcg ctttcctgcc actttagtgt
    14881 ttaccggcgg aaactatcgc ggcgtgctga atggtattca cccgtgggcg gaactgataa
    14941 acctgatgcg cgggcttgtc gaaccgcagc aggagcgtgc ctcatgagcg aaactttttt
    15001 ccatctgctg gggccaggaa cgcaaccgaa cgatgacagt ttcagcatga atccactgcc
    15061 gatcacctgt caggtgaatg atgaaccgag tatggcggcc ttggagcaat gtgctcacag
    15121 cccgcaggtg attgcgctgt taaacgagtt acaacatcaa ctaagcgaac gccagccgcc
    15181 gttgggcgag gtgctggcag tcgatctgtt aaatctcaac gccgacgatc gtcactttat
    15241 caatacgctt ctcggggaag gggaagtgtc agtgcgcatt cagcaggctg acgacagtaa
    15301 aagtgaaata caggaggcga tcttctgcgg attatggcgg gtgcgcagac gtcgcggcga
    15361 aaagttgctg gaggacaaac tggaggctgg ctgcgcgccg ctggcgttgt ggcaggcggc
    15421 aacgcaaaat ctcttgccga cagattcgct gttaccgccg cccattgatg gcctgatgaa
    15481 tggcctaccg ttggcgcatg agttactggc acatgtacgt aaccccgacg cgcagccgca
    15541 cagcattaat ctgacgcaat tacccatcag cgaggctgat cggctttttc tctcgcgtct
    15601 ctgtgggccg ggaaatattc agattcgtac cattggctat ggcgagagct atatcaacgc
    15661 cacggggtta cgccatgtct ggcatttacg ctgtacggac accttaaaag gcccgttact
    15721 ggaaagttat gaaatctgcc caataccgga agtggtgctg gcagcgccag aagatttggt
    15781 cgactctgcg cagcggctta gcgaggtatg tcagtggctg gcggaagctg caccgatgta
    15841 aaaagacggt aagtatcgct ttcagtctta tgaatatcgc aatcggcgaa tacctctggt
    15901 cgtagagttt caggataaag agggagatct aacattatcg ggttattttt ctctcttcgc
    15961 ctacaggagt gcgcatgtgg gatgtcattg atttatcgcg ctggcagttt gctctgaccg
    16021 cgctgtatca ctttttattt gtacccctta ccctggggct gatttttttg ctggctatca
    16081 tggaaaccat ttacgtggtc accggcaaaa caatctaccg cgatatgacg cgcttctggg
    16141 gtaagctctt cggtatcaat tttgctcttg gcgtggctac cggcctgacc atggagtttc
    16201 agtttggtac taactggtca ttctattcca actatgtggg cgatattttt ggcgcaccac
    16261 tggcgatgga agcattaatg gccttcttcc tcgaatccac ctttgtcggg ctgttcttct
    16321 tcggctggca acggctgaat aaataccagc acctgctggt gacgtggctg gtggcgttcg
    16381 gttcaaatct ctctgcattg tggatattga atgccaacgg ttggatgcaa tacccgaccg
    16441 gtgcgcattt tgatatcgac accctgcgta tggagatgac cagcttcagc gagctggtct
    16501 ttaacccggt cagccaggtg aaatttgtgc acaccgtcat ggcgggctac gtgaccgggg
    16561 ccatgtttat tatggcgatc agcgcctggt atttactgcg cggacgggag cgcgatgtcg
    16621 cattacgctc gtttgccatc ggttccgtct tcggtactct ggcgattatc ggtaccctgc
    16681 aactcggaga cagttctgcg tatgaagtcg cgcaagtaca accggtaaaa ctggcggcga
    16741 tggaagggga gtggcaaacg gaacctgctc ctgcaccgtt ccatgtggtt gcctggccgg
    16801 aacaggatca agagcgtaac gcctttgcca tcaaaattcc cgcgctgcta gggatcctcg
    16861 ccactcactc attagataaa cccgtgccgg gtctgaagaa tttgatggct gaaacctacc
    16921 cacgcttgca acgcggacgt atggcctggc tgttaatgca ggaaatatct caaggcaatc
    16981 gtgagccgca tgtgttgcag gcattccggg agctggaagg cgaccttggt tacggcatgt
    17041 tgctctcccg ctatgcgccg gatatgaatc atgtcacagc cgcacagtac caggcggcga
    17101 tgcgtggcgc gatacctcag gttgcgccgg tattctggag tttccgcatc atggtgggct
    17161 gtggttccct gctgctactg gtgatgctga ttgcgcttgt ccagacgctg cgtggcaaaa
    17221 tcgaccagca tcgctgggtg ctgaaaatgg cgctctggag tttgccgttg ccgtggattg
    17281 cgattgaagc cgggtggttt atgaccgagt ttggtcgtca gccgtgggcg atacaggaca
    17341 tcttaccgac atactccgcg cactccgctt taaccacagg acaactggct ttctcactga
    17401 tcatgatcgt agggctttac accctgttct taatcgccga agtctacctg atgcagaaat
    17461 atgcccgtct ggggccgagc gcgatgcaga gtgaacaacc gacgcagcaa caggggtaaa
    17521 ggagaaaatc atgtttgatt atgaaacatt gcgcttcatc tggtggctgc tgattggcgt
    17581 gatcctggtg gtctttatga tctccgacgg atttgacatg gggatcggct gtttgctgcc
    17641 gctggtggcg cgtaatgatg atgaacgccg gatagtgatt aatagcgttg gtgcgcactg
    17701 ggaaggcaat caggtctggt tgatcctcgc aggtggggca ttatttgccg cctggcccag
    17761 agtgtatgca gcggcgtttt ccggctttta tgtggcgatg atcctggtgc tgtgctcgct
    17821 gttcttccgt ccgctggcct ttgattatcg cggaaaaatt gccgatgcac gctggcgtaa
    17881 aatgtgggat gccggtctgg tcatcggcag tctggtgccg ccggtagtct tcggtatcgc
    17941 cttcggcaac ttgttgctcg gcgtgccgtt tgccttcaca ccgcaattac gcgtggagta
    18001 tctcggcagc ttctggcaac tgctgacgcc attcccttta ttgtgtggtt tgctcagcct
    18061 tgggatggtg attttgcaag gtggcgtctg gttacaactg aaaactgttg gtgtgattca
    18121 tctgcgttca cagctggcga ctaaacgcgc tgcactgttg gtgatgctgt gctttttgct
    18181 ggcaggttac tggctgtggg tcggtattga tggctttgta ctgctcgccc aggatgctaa
    18241 cggtccttcc aatccgttaa tgaaactggt ggcagtgcta cctggtgcct ggatgaataa
    18301 tttcgtcgaa tcgcccgttt tgtggatctt cccgctgctg gggttcttct gcccattgct
    18361 gacggtgatg gcgatttatc gtggtcgccc gggttgggga tttttgatgg catcattgat
    18421 gcaatttggc gtgattttca cggcaggcat cacgctgttc ccctttgtca tgccgtcaag
    18481 tgtgagtcca atctccagcc tgacgttgtg ggacagcact tccagtcagc tgacgctgag
    18541 cattatgttg gtaatcgtgc tgatattttt gcccattgtg ttgctctaca ctctctggag
    18601 ctactacaaa atgtggggac gcatgacaac agaaactctc cgccgtaacg aaaacgagtt
    18661 gtactaagga gcaggaacat gtggtattta ctttggttcg tcggcatttt gttgatgtgt
    18721 tcgctctcca cccttgtgtt ggtatggctg gacccgcgtc tgaaaagtta acgaacgtag
    18781 gcctgatgcg gcgcattagc atcgcatcag gcaatcaata atgttagata tgaaaagcgg
    18841 aaacatatcg atgaaagcga tcttaatccc atttttatct cttctgattc cgttaacccc
    18901 gcaatctgca ttcgctcaga gtgagccgga gctgaagctg gaaagtatgg tgattgtcag
    18961 tcgtcatggt gtgcgtgctc caaccaaggc cacgcaactg atgcaggatg tcaccccaga
    19021 cgcatggcca acctggccgg taaaactggg ttggctgaca ccgcgcggtg gtgagctaat
    19081 cgcctatctc ggacattacc aacgccagcg tctggtagcc gacggattgc tggcgaaaaa
    19141 gggctgcccg cagtctgctc aggtcgcgat tattgctgat gtcgacgagc gtacccgtaa
    19201 aacaggcgaa gccttcgccg ccgggctggc acctgactgt gcaataaccg tacataccca
    19261 ggcagatacg tccagtcccg atccgttatt taatcctcta aaaactggtg tttgccaact
    19321 ggataacgcg aacgtgactg acgcgatcct ctgcagggca ggagggtcaa ttgctgactt
    19381 taccgggcat cggcaaacgg cgtttcgcga actggaacgg gtgcttaatt tcccgcaatc
    19441 aaacttgtgc cttaaccgtg agaaacagga cgaaagctgt tcattaacgc aggcattacc
    19501 atcggaactc aaggtgagcg ccgacaatgt ctcattaacc ggtgcggtaa gcctcgcatc
    19561 aatgctgacg gagatatttc tcctgcaaca ggcacaagga atgccggagc cggggtgggg
    19621 aaggatcacc gattcacacc agtggaacac cttgctaagt ttgcataacg cgcaatttta
    19681 tttactacaa cgcacgccgg aggttgcccg cagtcgcgcc accccgttat tggatttgat
    19741 catggcagcg ttgacgcccc atccaccgca aaaacaggcg tatggtgtga cattacccac
    19801 ttcagtgctg tttattgccg gacacgatac taatctggca aatctcggcg gcgcactgga
    19861 gctcaactgg acgcttccag gtcagccgga taacacgccg ccaggtggtg aactggtgtt
    19921 tgaacgctgg cgtcggctaa gcgataacag ccagtggatt caggtttcgc tggtcttcca
    19981 gactttacag cagatgcgtg ataaaacgcc gctgtcatta aatacgccgc ccggagaggt
    20041 gaaactgacc ctggcaggat gtgaagagcg aaatgcgcag ggcatgtgtt cgttggcagg
    20101 ttttacgcaa atcgtgaatg aagcacgcat accggcgtgc agtttgtaat gcataaaaaa
    20161 gagcattcag ttacctgaat gctctgaggc tgatgacaaa cgaagaactg tctaatgcgt
    20221 agaccggaaa aggcgttcac gccgcatccg gtcactttca gttttactct ttctcggagt
    20281 aactataacc gtaatagtta tagccgtaac tgtaagcggt gctggcgcgt ttaatcacac
    20341 cattgaggat tgtgccttta atattaacgc cagcctgttc cagacgctgc attgacaagc
    20401 tcacctcttt ggcggtgttc aagccaaaac gcgcaaccag caggctggtg ccgacagagc
    20461 gtcccacgac cgcagcattg ctcaccgcca gcatcggcgg cgtatcgaca atcaccagat
    20521 cgtaatggtc gttcgcccat tccagtaatt gacgcatccg atcgcgcatc agcagttcag
    20581 acgggttagg tggcacctga ccgcgagtaa tcacatcaaa gcctcctttg ccaaaatgct
    20641 ggatcacttt gttgagctca tctttacctg ccagatattc cgacaagcca tgttcattac
    20701 tcacggtaaa caggttatgc gaataaccac ggcgtaagtc ggcatcaata aataacactt
    20761 tttgatcgga ctgggcgatc accgctgcca gagttgaact gacaaacgtt ttaccactgt
    20821 ctggcgtcgc accggtgatc atcagaatgt tattctcagt ctccatcata gcgaaatgca
    20881 gactggttcg tagcgcacgt acggcttcca cagcagaatc cgccgggtta tccaccgcca
    20941 ggaaggggat atttttagta cgatggcgct gctgattaga aaataaattt ttcttacgca
    21001 gacgggtgcg tttatccagc cactcggaca ttgggatagt ggaataaacg ctgatgccgt
    21061 gctcttccag ttgttccggg gcttctacac cacgacgcaa catcgcacgc gccagcacgg
    21121 cactcaccga aataaacagg ccaagaataa aaccaagcac cacattcaac gcttttttcg
    21181 gtttcactgg ctgcggctga gtgactgccg ggtcgataat ccgcacgtta ccaatggcac
    21241 tggatttcga aatactcaac tcctgctggc ggttaagtaa ttgcagatat accgcacggc
    21301 ccgcttctac gtcacgactt aaacgcaaca cttcctgttg ggtggaaggc attgccgata
    21361 cccgcttatt caggcgtttg cgttcttgct ccagcgtctg gcgtttttcc agcagcgcac
    21421 gataagttgg gtgatctttc ttatacagct gggagatctc tgcctcgcgg aaggtcagct
    21481 cattgagttg attatcaacg ttcacaatct gctcaagaac ggctttggct tccaggttaa
    21541 ggtcaaccga atcgcgctgc tggcgataaa cgttgagttt ttcttccgcc tggtccagct
    21601 cgctgcgcac ttcaggtaac tggcgctgta agaattcaag gctttgtgaa tcctgcgccg
    21661 cctggcgagc gatattctgt tgcaaatagt tgttagcgat gctgttcaga atacgagtaa
    21721 tcaactgggg atcatcacca gtcatggtaa gttccagcat cccgctttct ttactgcgtt
    21781 cgctaacggt aaaggtttcc tgcaatgcgt taatcgcttc cagttcggta cgctggctca
    21841 ggacaaactg tgttcctggt ttggccttaa tgtccgcgat agtcagcgca acgccatctt
    21901 tttccagacg ctggccgacc ataccattga cggtgaactc ttcaccttcc agtgtatagt
    21961 ggccgttttc cccaaccgtg agtgtcagtt gctgatcctg accattcagt tgtggaatat
    22021 gcatccagct gatcgccagc tcacctagtt tttctttggt taatctcgcc cagccgcgac
    22081 ccacaatcgg aaaatacttc tgctcaacta tgtcgcgcag attcagttca gcaatggttt
    22141 taccgagaat catgcgcgat tgcagcagtt ggatctccgg tgcagactcg ggcgatgagt
    22201 tagggatcat atcgctcagg ccgctgagaa tggcgttgcc ctgtttttgc tcaacctgga
    22261 ccagagtatc tgcctgataa attggtgtgc ttaacagcga gtaagcgaca gcgatcagcg
    22321 tgaataacgc ggtcacgctg ataataaact tacggtgatc ccataactcg ccgaccagac
    22381 gaagcagatc gatctcattt tcctgagtgc tgcctggtgg cgtattcata tttttagttg
    22441 tcatcgtatt ttataaactc aaattaccgg ctgaggcgct tcgcccattc ctgactggcg
    22501 cgctccaaca taccgtagac atgttcaaat gcgtcctgac ttttacgata gggatccggg
    22561 atctctttct gttccagcca ttgcccaaac agcattgtct tcccgcggac ctcgggagca
    22621 attgcggtaa cctgagcaat atgttccgac tccattgcca gaatcagatc gtaatttcgt
    22681 gccatctcag cagtgagctt acgtccggca tgtccttcaa gagatacgcc atgattagcg
    22741 gcaacatctg ccgctgtcgc atctgcaggg tgttttacca gaccatgaac acctgccgat
    22801 ttgactttta cgcccggcag acgtttacgc agcaagcgct cgccaatggg cgaacggcaa
    22861 atgttgccgg tacaaaccac taggattgag ttaaatttta gttgggccat gtttgaatcc
    22921 accgtgaagt ctctgtcagg tcatgcaccc cggaaatcgt cggtaccagc agagaaatca
    22981 cacggttcca acgagccagc ggtgcggtag tgacataaac aatgtcgtaa ggttcaagct
    23041 ggaattccgt tccgagtatc attgccgatg cgtccttcgc attcagctga taaatattgg
    23101 cgattttgcc attctgcttg ttttgcgttg cacgaataac aaaaatcccc gtcgcatcag
    23161 cgacatcctg attcataccc tcagcgttac ccagcgcttc tgccagggtc ataccgctgc
    23221 ggtccatttt cagcgtgctc tgtttaccca cttcacccat cacgaagact ttcagatcat
    23281 cgttgcgcgg aataaataaa atatcgcccg ggtgcagcag tttgttctgg cgcaagtcgc
    23341 cacgctgcat cagcgcatag aggttcactt tggtctttac gccattctgg gtgagcacca
    23401 cgttgcgcca gtctgcatct gcggtcagac cgcccgcggc gttaatcgca tccatgatgg
    23461 tcagaggaat attggtaatc ggctgctgac ccgatttgct gacttcgccg gtgacgtacg
    23521 ctttttgcga tcggaacgcc gcgacgctga catcgacttg cgggctttca atgactgaat
    23581 cgagacgcgc tgtaatctcg ttgcgtacct gggtaagtgt tttcccggcc actttcaggc
    23641 gaccaatata ggggtagaaa atcgcaccat cggcgttaac ccagttgccg gtatcactgg
    23701 cgctacggta ttgccctgcg ggggtggtta gttccgggtg atcccagacc gtaaccatca
    23761 acacatcacc aatcccgata cgatattcat agtttgcgat ttgctgctcc agctccgggt
    23821 tagcctgcga cataatcggt ttggcgcgca gctgatcaat caatcctggc gtgacgggat
    23881 agatattgac cattttgtcc aggtcatact gattgtcagg tagttcaatg acatccttgt
    23941 tactggtcga taaattctga ccaggaacaa gggtacatgc tgtcaaagac agaacagcca
    24001 atgtcagtac cgaaaactta aaaatgttct ttttcattgg tttatattta tcagtcaatg
    24061 taatgttatg aattacgtca ggttattgtc caaccggtac ttctcgctct gccgtcattg
    24121 gatcgagctg gtatttgcgt gacagcattt gtccaccatc acgcgtcaat ggtgtccacg
    24181 aaaccaccgc gcggttgcgg ttaggtccaa tggtcatcaa gtcgaacgga atcgagatat
    24241 aaaaaccttt actaaagccg ccttcgccgt agtcatcttt agatacgtta ctgattgccg
    24301 cccatacccc taccgccacg ccgctgtcaa agcgtttggc gacgtcgatc gttgcgcctt
    24361 tatcttttgc cagatattgc ccaacgctaa gtttcatcag tacgccattg agcgtcggcg
    24421 ggttccagta agccgtcacg aaacctgtgg gcgtggaata atcggtgaag cgcatcatgt
    24481 tgtcccagtc acgctgcttc acgtagttaa cgtccacacc cagcgcccag ctggcatcta
    24541 gcgggcgata aagaagctcg gaaccgacac cggcatacat cgtttccaga taaccgccat
    24601 acacctggcc atagaaacca ttgcctaagt cggcaaagta attcgcttgc aagttgttga
    24661 gataaacgtc attgcgaacg taatcacgga tatgcgtgcg cacgcggggc agggtagagt
    24721 ccgcgggtaa cagtgaagac ttaaacttgt cgtagttgtt gtaaatattg gtgaaaatac
    24781 cgtcatcaag cagcaggtgg tcggtaaacc agtagcgggc gctggacatc agccccagct
    24841 ggaacatata gaaatcttcc gggccgccca gcgactgtga aagtgttggg ttgaaagagt
    24901 agctaaagcg atcttcacga atacggtagc cccgaccaaa ggcagaaaga tcttctgctt
    24961 caacacgttg ttgttgcagt tgctctgatt taccaggcgc tgtgccggcc agctgtttgc
    25021 gcaggctggc tacgtcggtt tcggtagtca ccatcgccat atgctcgcgc ttttgcgtca
    25081 cgctaatctt ctcaacgcct tgcggcaggt tattcaccag aatccggttg gcacgatcga
    25141 ccgcttcgcg agagtcacgg tatttgtatt gctgaccaga catatacagc gtcttatcgc
    25201 gtagctgaat ttctggcgcg tcaaagcccg cgttatactt caggtgaata agttgtatat
    25261 ttaaaatctc tttgactatc aatgaattag tataagaagg tcattattag tcaaaataaa
    25321 atcatttgtc gatttcaatt ttgtcccatt cccgtccacg gctgtcccta taccgcgccg
    25381 ccattgaatc tgatttatgt ccgagaagac gttgagcaaa cttatcgcca atctgattac
    25441 ggtatagcct cgccgacagg ctacgcagtt catggaatgt tggcgggtct ccatcaaatg
    25501 agagtcctga tgcatttctc gcctttgtaa aatacttcga tactgttttc ggggaaagcg
    25561 gatcgtgatg ctttgatgcg attatagttt cactgctgct ggcctccctg catttctgta
    25621 gtgtatcagc caatgagata ttgagcgcgt caatcgttag cgtcagcgga atggcgagct
    25681 tagcccctgt tttgctctgt tcaatgtgaa gatggttg
//
LOCUS       DAJTQY010000056        24971 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.056, whole genome shotgun sequence.
ACCESSION   DAJTQY010000056 DAJTQY010000000
VERSION     DAJTQY010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24971
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.056"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            1..465
                     /locus_tag="OO349_002375"
                     /pseudo
     CDS             1..465
                     /locus_tag="OO349_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015695610.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
     gene            793..1875
                     /locus_tag="OO349_002376"
     CDS             793..1875
                     /locus_tag="OO349_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpD"
                     /protein_id="HCR5867419.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYVRLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNDQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLIEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGLVYQF"
     gene            complement(2364..3162)
                     /locus_tag="OO349_002377"
                     /pseudo
     CDS             complement(2364..3162)
                     /locus_tag="OO349_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(3172..3723)
                     /gene="ybcL"
                     /locus_tag="OO349_002378"
     CDS             complement(3172..3723)
                     /gene="ybcL"
                     /locus_tag="OO349_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HCR5867420.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPTDAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKVWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEITPVYEIK"
     gene            complement(3892..4224)
                     /gene="emrE"
                     /locus_tag="OO349_002379"
     CDS             complement(3892..4224)
                     /gene="emrE"
                     /locus_tag="OO349_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family multidrug efflux protein EmrE"
                     /protein_id="HCR5867421.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(4569..4883)
                     /gene="fliE"
                     /locus_tag="OO349_002380"
     CDS             complement(4569..4883)
                     /gene="fliE"
                     /locus_tag="OO349_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HCR5867422.1"
                     /translation="MSAIQGIEGVISQLQATAMSACAQESLPQPTISFAGQLHAALDR
                     ISDTQTVARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            5097..6762
                     /gene="fliF"
                     /locus_tag="OO349_002381"
                     /pseudo
     CDS             5097..6762
                     /gene="fliF"
                     /locus_tag="OO349_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
     gene            6755..7750
                     /gene="fliG"
                     /locus_tag="OO349_002382"
     CDS             6755..7750
                     /gene="fliG"
                     /locus_tag="OO349_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HCR5867423.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            7743..8429
                     /gene="fliH"
                     /locus_tag="OO349_002383"
     CDS             7743..8429
                     /gene="fliH"
                     /locus_tag="OO349_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HCR5867424.1"
                     /translation="MSDNLPWKTWTPDDLAPPPAEFVPMVESEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKAQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            8429..9802
                     /gene="fliI"
                     /locus_tag="OO349_002384"
     CDS             8429..9802
                     /gene="fliI"
                     /locus_tag="OO349_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HCR5867425.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPFPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            9824..10165
                     /gene="fliJ"
                     /locus_tag="OO349_002385"
                     /pseudo
     CDS             9824..10165
                     /gene="fliJ"
                     /locus_tag="OO349_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
     gene            10162..11289
                     /gene="fliK"
                     /locus_tag="OO349_002386"
     CDS             10162..11289
                     /gene="fliK"
                     /locus_tag="OO349_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HCR5867426.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLTLLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSDILADAQQADLLIPVDETLPVINDEQSTSTPLTTAQ
                     TMTLAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTADASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMISPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            11394..11858
                     /gene="fliL"
                     /locus_tag="OO349_002387"
     CDS             11394..11858
                     /gene="fliL"
                     /locus_tag="OO349_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HCR5867427.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            11863..12867
                     /gene="fliM"
                     /locus_tag="OO349_002388"
     CDS             11863..12867
                     /gene="fliM"
                     /locus_tag="OO349_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HCR5867428.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVEFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            12864..13277
                     /gene="fliN"
                     /locus_tag="OO349_002389"
     CDS             12864..13277
                     /gene="fliN"
                     /locus_tag="OO349_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HCR5867429.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSEKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            13280..13645
                     /gene="fliO"
                     /locus_tag="OO349_002390"
     CDS             13280..13645
                     /gene="fliO"
                     /locus_tag="OO349_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HCR5867430.1"
                     /translation="MNNHATVQSSAPVSAEPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRGGRS"
     gene            13645..14382
                     /gene="fliP"
                     /locus_tag="OO349_002391"
     CDS             13645..14382
                     /gene="fliP"
                     /locus_tag="OO349_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HCR5867431.1"
                     /translation="MRRLFSVAPVLLWLITPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVINKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            14392..14661
                     /gene="fliQ"
                     /locus_tag="OO349_002392"
     CDS             14392..14661
                     /gene="fliQ"
                     /locus_tag="OO349_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HCR5867432.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            14670..15455
                     /gene="fliR"
                     /locus_tag="OO349_002393"
     CDS             14670..15455
                     /gene="fliR"
                     /locus_tag="OO349_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliR"
                     /protein_id="HCR5867433.1"
                     /translation="MMQETSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPGSHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     SEPLNSNAFLAPTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEIFNLLADIISELPLI"
     gene            15740..16363
                     /gene="rcsA"
                     /locus_tag="OO349_002394"
     CDS             15740..16363
                     /gene="rcsA"
                     /locus_tag="OO349_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HCR5867434.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGNILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(16407..16595)
                     /gene="dsrB"
                     /locus_tag="OO349_002395"
     CDS             complement(16407..16595)
                     /gene="dsrB"
                     /locus_tag="OO349_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HCR5867435.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVALEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            16758..16985
                     /gene="yodD"
                     /locus_tag="OO349_002396"
     CDS             16758..16985
                     /gene="yodD"
                     /locus_tag="OO349_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HCR5867436.1"
                     /translation="MKTAKVYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            17283..18098
                     /locus_tag="OO349_002397"
                     /pseudo
     CDS             17283..18098
                     /locus_tag="OO349_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
     gene            complement(18095..19789)
                     /gene="yedQ"
                     /locus_tag="OO349_002398"
                     /pseudo
     CDS             complement(18095..19789)
                     /gene="yedQ"
                     /locus_tag="OO349_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
     gene            complement(19960..20142)
                     /locus_tag="OO349_002399"
     CDS             complement(19960..20142)
                     /locus_tag="OO349_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HCR5867437.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETDLVPGEGSRSAEEV"
     gene            complement(20221..21138)
                     /locus_tag="OO349_002400"
     CDS             complement(20221..21138)
                     /locus_tag="OO349_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HCR5867438.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHIAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            21311..22231
                     /gene="yedA"
                     /locus_tag="OO349_002401"
     CDS             21311..22231
                     /gene="yedA"
                     /locus_tag="OO349_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HCR5867439.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVGSWPPLMMAGVRF
                     QAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     IATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(22220..22690)
                     /gene="vsr"
                     /locus_tag="OO349_002402"
     CDS             complement(22220..22690)
                     /gene="vsr"
                     /locus_tag="OO349_002402"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HCR5867440.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRRREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(22671..24089)
                     /gene="dcm"
                     /locus_tag="OO349_002403"
     CDS             complement(22671..24089)
                     /gene="dcm"
                     /locus_tag="OO349_002403"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HCR5867441.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKMLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPEHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHQEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDKGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(24156..24696)
                     /locus_tag="OO349_002404"
                     /pseudo
     CDS             complement(24156..24696)
                     /locus_tag="OO349_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310727.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
     gene            complement(24736..>24971)
                     /locus_tag="OO349_002405"
     CDS             complement(24736..>24971)
                     /locus_tag="OO349_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5867442.1"
                     /translation="AFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLGSTAGKWLG
                     ALVGFLFMALVGALLGGIAWYTRPRPARCRRYE"
BASE COUNT         6309 a         6409 c         6153 g         6100 t
ORIGIN      
        1 tgttaattaa ctatcccccc aaactggcga tatccagtct ggtaaacaaa cagcctcaaa
       61 ggcgtatcgg gtaggttact gcgacgggat cgaccagata ttgctgccag gtattaccgc
      121 aaagatgttt tgtggcgtcc cggctatttt gccagtagct gcggaggtgc gccaatatcc
      181 gtcatccgcc aatacattga acaacagcaa acacctggtc agatgaaaaa acgagcctta
      241 tatccccgcc ctgaaggacg ggcttttacg gtgcactgga taagcctgga gattatctac
      301 acctgcatgg acatcattgt actatgcccc gtacatgccc catcacatcc tcaacatcaa
      361 agtattagag ccttgcaaac cattatgcgt ctcagtttgt ctcatctatc ttacgcaaaa
      421 aaaatagaac taatatcaag cggcgctata ttgcgtatta aataaaacca cctcaattac
      481 attaataaat ctaaataaat atcatgcaaa taatttccat ggttattttt aggctattta
      541 aattaaaaaa ataaattatg agtcaatcat aaataacaac ctttaatata atttgactac
      601 caaatttata ttcgttcaca aaatagtcat gcattgtgtg caatagaaac agttcagata
      661 aaaataggga tctacttcac aatttaaatg agaaactaaa acttacatct tgaaataatc
      721 tcattgattg gatgaatatt tgtagtgcag tgacatcatt tttaaataat agttcaaata
      781 aaagggctca cgatgaaaaa attaacagtg gcaatttctg ctgtagctgc atcagtactg
      841 atggcgatgt ctgctcaggc agcagagatt tacaataaag acagtaacaa gctggatctg
      901 tacggaaaag ttaatgctaa gcactacttc tcttctaacg atgcagatga tggcgacaca
      961 acttatgttc gtctgggctt caaaggcgaa actcaaatca acgatcaact gactggtttc
     1021 ggtcagtggg aatatgaatt caaaggcaac cgtgctgaat ctcaaggttc ttccaaagat
     1081 aaaacccgtc ttgccttcgc tggcctgaaa ttcggtgact acggctccat cgattatggc
     1141 cgtaactacg gtgtagcata cgatatcggt gcatggaccg acgttctgcc agaattcggt
     1201 ggcgatacct ggacccaaac agatgtgttc atgactggtc gcaccacagg tgttgcaacc
     1261 tatcgtaaca acgacttctt tggtctggtt gatggtctga actttgctgc tcagtatcag
     1321 ggtaaaaatg accgcactga cgtaactgaa gctaacggtg atggtttcgg tttctccact
     1381 acttatgagt atgaaggatt cggtgtaggt gcaacctatg ctaaatctga ccgcactaat
     1441 gatcaggtta tctacggtaa caacagcctg aatgcatctg gtcaaaatgc tgaagtatgg
     1501 gcagctggtc tgaaatatga tgcgaacaac atctatctgg ctaccaccta ttctgaaacc
     1561 cagaacatga ctgtttttgg taataaccat attgccaaca aagcacaaaa cttcgaagta
     1621 gttgcacaat atcagttcga cttcggtctg cgtccgtccg ttgcttacct gcaatctaaa
     1681 ggaaaagact tgggtgcgtg gggtgatcag gacctgattg aatatattga tgtaggtgca
     1741 acctattact tcaacaaaaa tatgtccact tttgttgatt acaaaatcaa cctgattgat
     1801 aagagcgatt tcacgaaagc atctggcgtt gctaccgatg atatcgttgc tgtaggattg
     1861 gtttaccagt tctaatctga ttactcaaag atatgttgtg ggaggctttg cctccccaac
     1921 atataagtgg cgctctcaag ccactttcct tagaagcact atcttgcttc tgactatata
     1981 aattaccctt tattttctgg gacgtttcaa cgccccattt ttaataatac tcagtgaaca
     2041 attaacgtat caattagatt taataacaac gatatccgta cctaacctga tatctaatgt
     2101 cataagcatc cctttaatta tcccctcaac ctcttgtaaa tttcccactc ttgagctcca
     2161 catagcccaa acgccggatg caaaaaaaat cacacgtgca tctctgaaat tgtgatggag
     2221 gaagtgagta tcaattatat gtgcttataa acgttcccga ttggcacaga agtcaccgga
     2281 ttgttcaggc tccaataggt tgagtatgtc gtatttattt tccaaaataa aaatcgatgc
     2341 tgttgctcct taataaaata cttttactct ctggacgctt ttcttagctc tcttggtgtt
     2401 atattaaaac gattatggaa cctttcagta aaacgagaag gacacttgta accattttcc
     2461 ctggcaatct cgctgatggg ttttactgtc gtttgtatag caggacaacg cattatttaa
     2521 cctcacgtcg tccaatatac tttgaaaact tattccctcg cttgccagac ggcgatgcaa
     2581 tgtggaaaca gaaatgtaga gatatctcgc taccttgttt gctgtccatt ttgttctagg
     2641 ttcggatagc attaggttat aacaacgact tattaatgat tgtttactat ctgataaaag
     2701 taaacaatta acatgatgtg ctcctaatga aagtagaacg cccattgcta agtgttcctg
     2761 aatttgcgtt gagaagcctc gggaaataga tgtttttagt tgctcccaac aatatattaa
     2821 ctcggggttc tgaggtaagc agaaacctgt cgtgttacga atttgatcag ttatcgcata
     2881 aaatttttga aacttttcaa ttagatcaat gggataataa agcatttctg caagataaag
     2941 acctgcttct ggataattca caatataaaa ttcatatcca cagggaaata atattatctg
     3001 attattatca acagttagac tatgcgtctc ccaattgata actttttttc cctgacggat
     3061 acgacataaa gctggcataa gaggcttaac cctgtgaatc tcatgatgtt tatgcatccg
     3121 tatttcttca atctttaggt tagacttatc tcttgccagc atactcttac cctattttat
     3181 ctcataaact ggtgttatct cagcggttgc aattttattg gcattaagca tataaccaac
     3241 taacgctccg ctggaattag aatccacagg aatcttatca gtttttagag cccatacttt
     3301 aaactggtaa tgatggggtt tatctccttt gggaggacat gctccaccaa atcctgcata
     3361 accaaaatca tttcggcctt gaacagcacc agtcggaagt tttgtaccat cacgtcttcc
     3421 tgcatcagtg ggcaaatatg ttacagttgc tggaatatta gcaacagtcc agtgccacca
     3481 tccactgcct gtaggagcat ctggatcata cacagttacg gcaaagcttt tggtgccttc
     3541 aggagcacca gaccaggtta atgagggcga tgtattacca ccttcacacc caaatccaga
     3601 aaatacatga gacgttgtaa gttgctctcc tgtttttatt tcattactag tgacctgaaa
     3661 tgctgcggcc tgcgcagaaa atgttatgaa tgccaataca cttgaaacga taagtttttt
     3721 cataaaaacc tcattgttgt gacctatcgt tattttattt gatgtgcctt gatctcatta
     3781 tgaataaagg cgcaatgatc gcgcaaatgc aatcataatt ataaaggtgc cccgaacatt
     3841 agccataata tgcacaaatc agattcctgt tatctcaagg agaattattt tttaatgcgg
     3901 tgcacttcgt gacaacaagt taatgactaa cacaccggca caaatcaaca tcatgcctat
     3961 gatagctggc aggtccagtc gttgaccaaa aaatccccag gaaagcaaac taatcagaac
     4021 gataccgact cctgaccaga tagcataagc aatccctgta ggtatataag ccagcgtctg
     4081 agctaataac cagaatgatg cacaatagca aataattgtg ccaacagagg gccataaccg
     4141 tgtaaacccc tctgaaaact tcattaaggt tgtaccaatg acctctgcaa gtattgcgcc
     4201 accaagataa atataagggt tcatagcata ttctttcctg ttcatactgg agagaattgt
     4261 actacagttt gaacacaact caccgttttc atcctggtgt atgtgtatcc aaggggctct
     4321 ggcgtggcaa gagataaagg caggaacaat aatgatcaga aggaacccca ggctaacgaa
     4381 tgagttcact cttcctggga gcaaagcgaa gacaaaacat gttatcgcgt ttacggatcg
     4441 tggatggcag aaaacaacca ggatcaggta tccattttaa accagaaatt gagtgagttt
     4501 gccccatcca tgccccacgc tgtgggatcg gatataatta aacaagcata gtcaacaggt
     4561 tacacaacct acacctgcat ggacatcact tcctgatacg ccgccaccag cttattacgc
     4621 acctgaatcc ccatttgcat agaaactgag gctttttgca tatcggtcat cacatcgttt
     4681 agcgccacgc cgggttcacc gagggtgaat ttttctgcct gcgtgcgggc gactgtttgt
     4741 gtatcgctta tgcgatcgag cgcggcgtgc agctgcccgg caaaactaat ggtcggttgc
     4801 ggcagtgatt cctgcgcaca cgcactcatc gccgtagcct gtaactggct gataacccct
     4861 tcaatcccct gtatcgctga cattttcgtc tcccggataa tttctggtaa caaagcctac
     4921 cagtaagtca ataagacaaa ggcgctaaat agcaacaaaa aaactggttt attggcggat
     4981 agaaaaaacg aaagcacaaa taatgggagc gtcaattttt cgagtttgct gacccgagag
     5041 tgagtcttgt tccactttgc caataacgcc gtccataatc agccacgagg tgcgcgatga
     5101 atgcgactgc agcccagaca aaatctcttg agtggcttaa tcgcctgcgt gcgaatccga
     5161 aaattccatt gattgttacc ggttccgcgg cagtggcggt catggtcgca ctgatcctgt
     5221 gggcgaaagc ccccgactac cgcacattat tcagcaatct ttccgatcag gatggtggcg
     5281 caattgtcag ccaactgacg caaatgaata ttccttaccg cttcagcgaa gccagcggcg
     5341 ctattgaggt tccggcagat aaagttcacg aactgcgcct gcgcctggcg caacaaggtt
     5401 tgcccaaagg cggcgcggtc ggtttcgaac tgctcgatca ggaaaagttt ggtatcagcc
     5461 agttcagcga acaggtgaat tatcagcggg cgctggaagg cgagctttct cgtaccatcg
     5521 aaactatcgg cccggtaaaa ggggcgcgcg tacatctggc aatgccgaaa ccgtctttat
     5581 tcgtccgtga acaaaaatcc ccttctgcat cggtgacggt aaatctgtta cccggccgcg
     5641 cactcgatga agggcaaatt agcgctattg tacatctggt ttccagcgcg gttgctggtc
     5701 tgccgcctgg aaacgtcacg ctggtggatc agggcgggca tctgttaacc cagtccaata
     5761 ccagcgggcg cgatcttaat gacgctcagt tgaaatatgc cagcgatgtc gaaggccgta
     5821 ttcagcagcg tattgaagcg atcctgtcac ctattgttgg taacggtaat attcacgcct
     5881 aggtcacggc gcaactggac ttcgccagta aagaacaaac ggaagaacag tatcgcccta
     5941 acggtgatga atctcatgcg gcgcttcgtt cacgccagct taatgagagc gagcaaagcg
     6001 gttccggtta tccgggcggc gtaccggggg cgttgtcgaa tcaaccggca cctgcgaata
     6061 acgcgccaat cagcacgcct ccgacaaatc aaaataaccg ccagcagcag gcgagcacca
     6121 ccagcaatag tgggccgcgt agcacacagc ggaatgaaac cagtaactac gaagtcgatc
     6181 gcaccattcg tcataccaaa atgaacgtgg gcgatgtgca acgtctgtca gtcgcggtag
     6241 tggtgaatta caaaaccttg ccagacggta aaccattgcc tctcagcaac gaacagatga
     6301 agcaaattga agctctgacc cgcgaggcga tgggcttttc tgaaaaacgc ggtgattcgc
     6361 tcaatgttgt taactcgccg ttcaatagca gtgacgaaag cggcggagca ctgccgtttt
     6421 ggcaacagca agtgtttatc gatcagttgc tggctgccgg tcgctggttg ctggttgctg
     6481 gtactgctgg tggcgtggtt gctgtggcgg aaagcggtac gcccgcagct aacacgtcgc
     6541 gccgaggcgg tgaagactgt gcagcaacag gcgcaggccc acgaggaagt ggaagatgcg
     6601 gtggaagtcc gtctgagcaa agacgaacaa ctccaacaac ggcgcgctaa ccaacgactg
     6661 ggggcagaag tcatgagcca gcgtatccgt gaaatgtctg ataacgatcc gcgcgtggtg
     6721 gcgctggtca ttcgccagtg gataaataac gatcatgagt aacctgacag gcaccgataa
     6781 aagcgtcatc ctgctgatga ccattggcga agaccgggcg gcagaggtgt tcaagcacct
     6841 ctcccagcgc gaagtgcaaa ccctgagcgc tgcaatggcg aacgtcacgc agatctccaa
     6901 caagcagcta accgatgtgc tggcggagtt tgagcaagaa gctgaacagt ttgctgcact
     6961 gaatatcaac gccaacgatt atctgcgttc ggtattggtc aaagctctgg gtgaagaacg
     7021 tgccgccagc ctgctggaag atattctcga aacccgcgat accgccagcg gtattgaaac
     7081 gctcaacttt atggagccgc agagcgccgc cgatctgatt cgcgatgagc atccgcaaat
     7141 tatcgccacc attctggtgc atctgaagcg cgcccaggcc gccgatattc tggcgttgtt
     7201 cgatgaacgt ctgcgccacg acgtgatgtt gcgtatcgcc acctttggcg gcgtgcagcc
     7261 agccgcgctg gccgaactga ccgaagtact gaatggcttg ctcgacggtc agaatctcaa
     7321 gcgcagcaaa atgggcggcg tgagaacggc agccgaaatt atcaacctga tgaaaacgca
     7381 gcaggaagaa gccgttatta ccgccgtgcg tgaattcgac ggcgagctgg cgcagaaaat
     7441 catcgacgag atgttcctgt tcgagaatct ggtggatgtc gatgatcgca gcattcagcg
     7501 tctgttgcag gaagtggatt ccgaatcgct gctgatcgcg ctgaaagggg ccgagcagcc
     7561 actgcgcgag aaattcctgc gcaatatgtc gcagcgtgcc gccgatattc tgcgcgacga
     7621 tctcgccaac cgtggtccgg tgcgtctgtc gcaggtcgaa aacgaacaga aagcgattct
     7681 gctgattgtg cgccgtcttg ccgaaactgg cgagatggtg attggcagcg gcgaggatac
     7741 ctatgtctga taatctgccg tggaaaacct ggacgccgga cgatctcgcg ccaccaccgg
     7801 cagagtttgt gccaatggtc gagtcggaag aaaccattat tgaagaggcc gaacccagcc
     7861 ttgagcagca actggcgcaa ctgcaaatgc aggcccacga gcagggttat caggcgggga
     7921 ttgccgaagg tcgccagcaa ggtcacgaac agggctatca ggaaggactg gcccaggggc
     7981 tggaacaagg tctggcagag gcgaaggctc aacaagcgcc aattcatgcc cggatgcagc
     8041 aactggtcag cgaatttcaa actacccttg atgcgcttga tagtgtgata gcttcgcgcc
     8101 tgatgcagat ggcgctggag gcagcacgtc aggtgatcgg tcagacacca acggtggata
     8161 actcggcact gatcaaacag atccaacagt tgttgcagca agaaccgtta ttcagcggga
     8221 aaccacagct gcgcgtacac ccggatgatc tgcaacgtgt ggatgatatg ctcggcgcaa
     8281 ctttaagttt gcatggctgg cgcttgcggg gcgatcccac cctccaccct ggcggatgta
     8341 aagtctccgc cgatgaaggc gatctcgacg ccagtgtcgc cactcgctgg caagaactct
     8401 gccgtctggc agcaccagga gtggtgtaat gaccacgcgc ctgactcgct ggctaaccac
     8461 gctggataac tttgaagcca aaatggcgca gttgcctgcg gtacgtcgct acgggcgatt
     8521 aacccgcgct accgggctgg tgctggaagc caccggatta caattgccgc tcggcgcaac
     8581 ctgtgtcatt gagcgccaga acggcagcga aacgcacgaa gtagaaagcg aagtcgttgg
     8641 ctttaacggt caacggctgt ttttaatgcc gctggaggaa gtggaaggtg tcctgcccgg
     8701 cgcgcgtgta tatgccaaaa acatttcggc agaagggctg caaagcggca agcagttgcc
     8761 gctcggtccg gcgttattag gtcgtgttct ggacggtagc ggtaaaccgc tcgatggcct
     8821 gcccttcccc gatacgacgg aaaccggtgc gctgattacc ccgccattta acccgttgca
     8881 acgtacaccg attgaacatg tgctggacac cggcgtgcgc ccaatcaatg ccctgcttac
     8941 cgtcggacgc gggcagcgta tggggctgtt tgccgggtcg ggcgttggta aaagtgtgct
     9001 gctggggatg atggcacgct acacccgcgc cgatgtcatt gtcgtgggtt tgattggtga
     9061 acgtgggcgc gaagtaaaag attttattga gaatatcctc ggtgccgaag gacgtgcacg
     9121 ctcagtggtg attgccgctc cggcggatgt ttctccgctc ctgcgaatgc aaggtgccgc
     9181 ctatgccacg cgcattgccg aagattttcg cgatcgtggt cagcatgtgt tgctgattat
     9241 ggactccctc acccgctacg cgatggccca gcgtgaaatt gcgctggcga ttggcgaacc
     9301 acctgccact aaaggttatc caccgtcggt gtttgccaaa ttaccggcac tggtcgagcg
     9361 tgccggtaat ggcattagcg gcggcggctc gattaccgcg ttttataccg tgctcaccga
     9421 aggcgatgac cagcaggacc ccattgctga ctccgcgcgg gcaatcctcg acggtcacat
     9481 tgtgctgtct cgccgactgg cggaagccgg gcactatccg gctatcgata ttgaagcgtc
     9541 gatcagtcgc gcaatgacgg cgttgatcag tgagcaacat tacgcgcgag tgcgcacctt
     9601 caaacagctg ttgtcgagtt ttcagcgtaa ccgcgatctg gttagcgtcg gcgcgtatgc
     9661 caaaggcagc gatccgatgc tcgataaagc catcgccctg tggccgcagc tggagggcta
     9721 tttgcaacaa ggcatttttg aacgcgcgga ctgggaagcg tctctccagg ggctggagcg
     9781 tattttcccg acagtgtcat aacccaggag ataacggcag atggcggaag aacagctcaa
     9841 aatgttgatc gattatcaga atgaatatcg caataacctc aatagcgata tgagtgccgg
     9901 gatgaccagc aaccgctgga tcaactatca gcagtttatc cagacgctgg aaaaagccat
     9961 tactcagcat cgccagcaac ttaatcagtg gacgcagaaa gttgacattg ccctgaacag
    10021 ctggcgagaa aagaaacaac gtttgcaggc ctggcagaca ctgcaggaac ggcaatccac
    10081 ggcggcactg cttgcagaaa accgcctcga tcagaaaaag atggatgagt tcgcccagcg
    10141 cgccgccatg aggaaacctg aatgattcgc ttagcgccct tgattaccgc cgacgttgac
    10201 accaccacat tgcctggcgg caaagccagc gatgctgcac aagattttct cacgttgttg
    10261 agcgaagcat tagcaggcga gacaacaact gacaaagcgg ccccccagtt gctggtagca
    10321 acagataagc ccacgacaaa aggcgagccg ctggtcagcg atattcttgc cgatgcgcaa
    10381 caagcggatt tactgatccc tgtggatgaa acacttcctg tcatcaacga cgaacaatcc
    10441 acatcaacac cgttaaccac cgctcagacg atgacgttgg ctgcggtggc tgacaaaaat
    10501 acgacaaaag acgaaaaagc ggatgatctt aatgaagacg tcaccgcaag tctgagcgcc
    10561 ctttttgcga tgttgccggg ttttgacaat acgcccaaag tgactgatgc accgtcaacc
    10621 gtgttaccgg cagagaaacc aacgctcttc acaaaactga cttctgcgca actcactaca
    10681 gcacagcctg atgacgcccc cggcacacca gctcagccat taacaccgct ggtagcagaa
    10741 gcccagagta aagcggaagt catcagcacg ccttcgccgg tgaccgctga cgccagcccg
    10801 ctaatcactc cacaccagac acagccactg cccaccgtcg ccgcgcctgt tttgagtgca
    10861 ccgctgggtt ctcacgaatg gcaacaatca ttgagccagc atatttcgct gttcacccgc
    10921 caggggcaac aaagtgcaga gttgcgtctg cacccacagg atttaggtga agtgcaaatt
    10981 tccctcaaag tggatgataa ccaggcgcag atccagatga tttcaccgca tcagcacgta
    11041 cgcgccgccc tggaagcagc gctgccggta ctgcgtacgc agctggccga aagtggcatt
    11101 cagttagggc aaagcaacat tagtggcgaa agctttagtg gtcagcagca ggccgcttcc
    11161 cagcaacagc aaagccaacg cacagcaaac catgaacctc tggcggggga agacgacgat
    11221 acgcttccgg ttcccgtctc tttacaaggg cgtgtaacag gtaacagcgg cgttgatatt
    11281 ttcgcctaac gtcagaggta gcaccgtaat ccgcgtcttt tccccgcttt gttgcgctca
    11341 agacgcagga taattagccg ataagcagta gcgacacagg aagaccgcaa cacatgactg
    11401 attacgcgat aagcaagaaa agcaagcgat cgctttggat cccgattctg gtattcatta
    11461 ccctcgcggc ctgtgccagc gcaggttaca gctactggca ttcgcatcag gttgccgctg
    11521 acgacaaagc gcagcaacgc gtcgtgccct ccccggtctt ctatgcgctg gataccttta
    11581 cggtcaattt gggcgatgcg gatcgcgtgc tttatatcgg cataaccctg cgactgaaag
    11641 atgaagcaac ccgctcgcgg ctgagtgagt atttgccgga agtccgtagt cgcttgctgt
    11701 tactgttttc acgtcaggat gctgccgtac tggcgacaga agaaggcaag aaaaacctga
    11761 ttgccgagat taaaaccaca ctttccaccc cgcttgttgc cgggcaaccg aaacaggatg
    11821 tcaccgacgt gctgtatacc gcttttattc tgcgataacg acatgggcga tagtattctt
    11881 tctcaagctg aaattgatgc gctgttgaat ggtgacagcg aagtcaaaga cgaaccgaca
    11941 gccagtgtta gcggcgaaag tgacattcgt ccgtacgatc cgaataccca acgacgggtt
    12001 gtgcgcgaac gtttgcaggc gctggaaatc attaatgagc gctttgcccg ccattttcgt
    12061 atggggctgt tcaacctgct gcgccgtagc ccggacatta ccgtcggggc catccgcatt
    12121 cagccgtacc atgaatttgc ccgcaacctg ccggtgccga ccaacctgaa cctaatccac
    12181 ctgaaaccgc tacgcggcac agggctggta gtgttctcgc cgagtctggt gtttatcgcc
    12241 gtggataacc tgtttggcgg cgatggacgc ttcccgacca aagtggaagg tcgcgagttt
    12301 acccatactg aacagcgcgt catcaaccgt atgttgaagc tggcgctgga aggctatagc
    12361 gacgcctgga aagcgattaa tccgctggaa gttgagtacg tgcgttcgga aatgcaggtg
    12421 gaatttacca atatcaccac ctcgccgaac gacattgtgg ttaacacgcc gttccatgtg
    12481 gagattggca acctgaccgg cgaatttaat atctgcctgc cattcagcat gatcgagccg
    12541 ctacgggaat tgttggttaa cccgccgctg gaaaactcgc gtaatgaaga tcagaactgg
    12601 cgcgataacc tggtgcgcca ggtgcagcat tcacagctgg aactggtggc caactttgcc
    12661 gatatctcgc tacgcctgtc gcagatttta aaactgaaac ccggcgacgt cctgccgata
    12721 gaaaaacccg atcgcatcat cgcccatgtt gacggcgtcc cggttctgac cagtcagtac
    12781 ggtactctca acggtcagta tgcgttacgg atagaacatt tgattaaccc gattttaaat
    12841 tctctgaacg aggaacagcc caaatgagtg acatgaataa tccggccgat gacaacaacg
    12901 gcgcaatgga cgatctgtgg gctgaagcgt tgagcgagca aaaatcaacc agcgaaaaaa
    12961 gcgctgccga cgcggtgttc cagcaatttg gcggtggtga tgtcagcgga acgttgcagg
    13021 atatcgacct gattatggat attccggtca agctgaccgt cgagctgggc cgtacgcgga
    13081 tgaccatcaa agagctgttg cgtctgacgc aagggtcagt cgtggcgctg gacggtctgg
    13141 cgggcgaacc gctggatatt ctgatcaacg gttatttaat cgcccagggc gaagtggtgg
    13201 ttgttgccga taaatatggc gtgcggatca ccgatatcat taccccgtcc gagcggatgc
    13261 gccgcctgag ccgttagtga tgaataacca tgctactgtg caatcttccg cgccggtttc
    13321 tgctgagcca ctgctgcagg tgagcggcgc actcatcgcc attattgccc tgatcctcgc
    13381 tgctgcctgg ctggtaaaac ggctgggatt tgcccctaaa cgcactggcg ttaacggtct
    13441 gaaaattagc gccagtgctt cgctgggcgc gcgtgaacgg gtcgtggtgg tcgatgtgga
    13501 agatgcacgg ctggtgctcg gcgttaccgc agggcaaatc aatctgctgc ataaacttcc
    13561 cccttcagca ccaacggaag agataccgca gaccgatttt cagtcggtca tgaaaaattt
    13621 gcttaagcgt ggcgggagat cctgatgcgt cgtttgtttt ctgtcgcacc ggtccttctc
    13681 tggctgatta cgcccctcgc cttcgcgcaa ctgccgggta tcaccagcca gccgctacct
    13741 ggcggtggac aaagctggtc gctcccggtg cagacgctgg tgttcatcac ctcgttgacg
    13801 ttcattccag cgattttact gatgatgacc agtttcaccc gcatcattat tgtttttggt
    13861 ttattgcgta acgcgctggg aacgccctcc gcgccaccta accaggtatt gctggggctg
    13921 gcactgtttt tgaccttttt tattatgtcg ccagtgatca acaaaattta tgtcgatgcg
    13981 taccagccat tcagcgaaga gaaaatctca atgcaggagg cgctggaaaa aggggcgcag
    14041 ccgctgcgtg agtttatgct gcgtcagacc cgcgaggcag atttagggtt gtttgccaga
    14101 ctggcgaata ccggcccgtt gcaggggcct gaagctgtgc cgatgcgcat tttgctcccg
    14161 gcctacgtga ccagcgagtt gaaaaccgca tttcagatag gcttcacgat tttcatccct
    14221 tttttgatta tcgacctggt gattgccagc gtgttgatgg cattggggat gatgatggtt
    14281 ccccccgcca ccattgctct gccctttaaa ctgatgctgt ttgtactggt ggatggctgg
    14341 caattgctgg tcggttcgct ggcgcagagc ttttacagct agagaggcaa aatgacacct
    14401 gaatcggtca tgatgatggg aactgaagcg atgaaagtcg cgctggcact ggcagccccg
    14461 ctgttgctgg tagcgctggt cacgggcctt atcatcagta ttttgcaggc agccacgcag
    14521 attaacgaaa tgacgctgtc gtttattccg aaaatcatcg ccgtatttat cgccatcatt
    14581 attgccggac cgtggatgct caatctgttg ctggattacg tccgcacctt gttcactaac
    14641 ctgccgtata tcatcgggta gcccgtacaa tgatgcagga gacaagcgat caatggcttt
    14701 cctggttaag cctgtacttc tggccgttac tgcgcgtgct ggcgctcatt tctaccgcgc
    14761 caattctgag cgaacgcagc gtgccgaaac gggtaaaact gggtctggca atgatgatca
    14821 cgttcgccat tgccccatca ttacctgcca acgatgttcc tgttttttcg ttctttgctc
    14881 tgtggctggc cgtacagcag atcctgatcg gcattgcgct tggttttacc atgcaatttg
    14941 cctttgccgc tgtgcgaacc gctggcgaaa ttatcggtct gcaaatgggg ctgtcatttg
    15001 cgacatttgt cgatcctggc agccatctca atatgcccgt tttagcgcgc atcatggata
    15061 tgctggcatt actgctgttc ctgacattta acggtcattt atggttgatt tcactgctgg
    15121 tcgatacctt tcacaccctg ccgattggta gcgaaccgtt gaacagcaat gcgtttctgg
    15181 cacccaccaa agcagggagt ttgattttcc ttaacgggct gatgctggcg ttaccgctca
    15241 ttactctgtt gctgaccctg aatctggcat taggtttact gaatcgtatg gccccgcaat
    15301 tatccatttt tgttattgga tttccattaa ctctgactgt cggcatctct ttaatggcgg
    15361 cattaatgcc gttaattgcg cctttttgcg aacatttatt cagtgaaatt tttaatttgc
    15421 tggctgatat tattagtgaa ttaccattaa tataattccg taacgtttat catgctaccc
    15481 taaggattat ccgaaaaata atatctgcga acatcttcca ggatactcct gtagcgaaat
    15541 atttgtttta agctcactca catatcgcaa catttacttt aagacaattc caagcaaatt
    15601 atacaacact ttacgggata gtaagtccgc ctgaaaaatt gcgagagtgg cgcattaggt
    15661 gacccatgtt gttccgttta gtcatgatga aatattcagg taaggggaat tatcgttacg
    15721 cattgagtga gggtatgcca tgtcaacgat tattatggat ttatgtagtt acacccgact
    15781 aggtttaacc gggtatctgt tgagtagagg ggttaaaaaa agagaaatca acgacattga
    15841 aaccgttgat gaccttgcca tagcttgtga ttcacagcgt ccttcagtgg tgtttattaa
    15901 tgaggactgt ttcatccacg atgcttctaa cagtcagcgc atcaagcaca tcattaatca
    15961 acatcccaat acgttattta tcgtttttat ggcaattgcc aatgttcatt ttgatgaata
    16021 tctattggtc agaaaaaatt tattgatcag ttctaaatcg attaaaccgg aatctctcga
    16081 cgatatcctt ggcaatattc tgaaaaaaga gacaacgata acctcgtttt taaatatgcc
    16141 gacgttatca ttgagccgaa ccgaatcgag tatgttgcga atgtggatgg caggtcaggg
    16201 aaccattcaa atctccgacc aaatgaatat caaagccaag accgtttcat cgcataaagg
    16261 taatattaaa cgtaagatca aaacgcataa taaacaggtt atctaccatg tcgtccgact
    16321 gacggataat gtgactaatg gtatttttgt caacatgcgc taacacattc tgactggtga
    16381 tttcccacca gtcaggctga ataagattac tctgctttct caacaaagat accgtcctga
    16441 tgccctgctt cattaaagaa ccagatgccg agcgggtagt cttccagcgc aaccaggtac
    16501 attgtgcctt cactaaactc ctcaactgcc agtaccactc caggacgacg cggaccgcca
    16561 tccgttttga ctgttacccg atcattcacc ttcatcgttt tcctcctgtg gctttgtgcc
    16621 agtgtagaac aatttcgttt ttctggcagc gccaagcgcg cgcgagtgct gattttctcg
    16681 acggtctata cttaagagat gctaggcgga cttaacgact ggcggcaaca acagagtaac
    16741 ggttgcgagg aaagatgatg aaaaccgcaa aagtgtacag cgataccgca aaacgtgagg
    16801 tcagcgtcga tgtcgatgcc ctgctggcgg cgatcaatga aattagcgaa agcgaagttc
    16861 atcgcagcca gaacgattct gaacacgtta gcgtcgatgg acgtgaatat catacatggc
    16921 gtgaattggc ggatgccttc gaactggata ttcatgactt cagcgtctct gaagtgaatc
    16981 gttgaattca caataaaaaa atcccgaccc tgagggtgtc gggatgaaac ttgcttaagc
    17041 aagaagcact taaaaaattc gttacaccag gaaatctgat gtgttcatca ccttatctgc
    17101 aatttttttc gctgacaaga aaatattcgc gctatgaatg attaacgcta cttatgaata
    17161 gaaatgtgac gcgcgtcacc ccttggtggc tctggtgtca acgggatcgc tcccgaaaaa
    17221 atcatctttg attatttgtg ctgttttgag aatcatcctg gtaaggggag tactgcgcga
    17281 ccatgttttc aattcaacaa ccactactgg tttttagcga tcttgatggc accctgctgg
    17341 acagtcatag ttatgactgg caaccggcag ccccctggct cagccgttta cacgaagcca
    17401 atattcccgt cattctctgt agcagtaaaa ctttagcgga aatgctgtac ttgcaaaaaa
    17461 tgttggggct acaaggctta ccgctgattg cagagaatgg cgcagtgatc cagcttgctg
    17521 aacaatggca ggatatagac ggttttccac gcatcatctc aggtattagc catggcgaaa
    17581 tctgccaggt tttaaatacg ctacgcgaga aagaacattt taaattcacg acttttgatg
    17641 atgtcgacga tgcaaccatt gccgaatgga cgggattaag ccgtagctag gcggcgctga
    17701 cgcagctgca tgaggcgtcg gtaacgctaa tctggcgcga cagtgacgag cgtatggcac
    17761 aatttatcgc tcgtctgaac gaactgggct tgcagtttat gcagggtgcg cgcttctggc
    17821 acgtactgga tgcctctgcc ggaaaagatc aggctgccaa ttggattatc gcgacctatc
    17881 aacaattgtc aggcagacgc ccaaccacac ttggcctggg cgatgggcca aacgatgcgc
    17941 ctttactgga ggtaatggat tacgcggtga ttgtgaaagg gctaaaccgt gaaggggtac
    18001 atctgcatga tgaggatccg gcccgcgtct ggcgaacgca gcgtgaagga ccggaaggct
    18061 ggcgtgaagg gctggaccat tttttctccg cccgttaagc gttatcgctc gcgaataccc
    18121 gattgcgccc ggcctgtttt gcgaggtaaa gccgacggtc ggccaatgac tggagttgtt
    18181 caaaatcata atcaccggtt tcctcgctgc tacttacccc cagcgaggca ctgatgcgta
    18241 tcgttgtact cttggcgatc agcatctctt tttcatttaa cttcaggcga atacgttctg
    18301 cgacctccgc agcctgcgtc agattcgcgc ctggcagaat cacacaaaac tcctcaccac
    18361 cgacccgccc ggcaacgtcc tgcgcacgca aggaactgct aattaatccg gcagcatgag
    18421 aaagaacacg gtcgcccgcc tgatgaccaa agcggtcatt aatcgcttta aaatggtcaa
    18481 gatcgacctg aatgacagaa aaaggatgtt ggtgcgtctg acacaattta gcgagcggac
    18541 gggctttttc gaacagtgcg ccacggttat ataaacgcgt taaggtgtcg tgccacgcct
    18601 gccactgcaa cgagctttgc agaacataca tgttgctgac catccggcga atcacatacc
    18661 aggagaggag taacatggtg gtaaagagcg cccacagcag ggttaatgca atgctgatac
    18721 tgccgaaatc gccgcgcacg ccttcgctta gcgtatggac gcgcgccagc acaccgtcga
    18781 aatggtccag acgttcccag ctaacatagc gactgtccat acgaatgccg ccccgcgtgt
    18841 catgttccat tgcctgcgcc agcaaggcca gttcacgagg atcaaaaata ttccctgttg
    18901 gatggtcagg attggaagag gtcaaaaatc tcagcttact gtcatagagc tgatactcac
    18961 catcgaggtt tttatcgatg gcgtttctta aaaattgctg catagtacgc acgggaatac
    19021 tcatccccag cacgccatac cagtagttat tactgtctac cggaacactg acggtaacct
    19081 gcggttcagt attgctggcg tgttccggtt gcgaagtgaa ccagcgtacc gcgcggtgac
    19141 gattttctcg ttgcgaatgg ccgataaacc agggttgggt gacatagccg taataacgcg
    19201 ttggtacatt gcgcgtaaac aaggtcggct gcgtcgaaac gtaaaatcca gcacgcgaga
    19261 catacatcgc ctgttcaacc atcgacgagg agttgtgcgc cagtcgcagc aagtaaccaa
    19321 cttccagtgc agcggtaatt tcattgtcga ggctttcatt ttcgcgagac aggaaattcc
    19381 cctcgctgac taatgcatcc gatacgccat tgactggcag ggtgcgccgt cggttgagtt
    19441 cgatttgcca ggcgtgctcg tcgcgatgct gttcgaactc ggttacggca ttacgcagag
    19501 aggtgaaatc cagtggcgcc acgagagctt cgcgcatgcc attacgcaaa aagatcagtt
    19561 tgtcgacgtt atactgcaaa tgcttatcga gcgcgttggc tacgttttcc agatgattac
    19621 gctagctgga gatataggca tcttccagca ccaccacttc acgccaggtg agcaaggtgg
    19681 aaaaaagcaa taccacgata aagcagagat taacgacatg accgggcccc aggcggcgtg
    19741 cgagtttttt caaccagctc tggttttcca tttttgtctc gtgctgcacc ctgacgactc
    19801 ccaacctgct tttttatgat tctggcataa cccggcaata gcgtaccgca aaacagcgat
    19861 aattttataa tggtggcatg ataagacgcg actatcattc attgcaggca ttgggacacc
    19921 aggccggatg tgatccggcc tggtgttaat gatacccttt caaacttctt ccgcagaacg
    19981 actcccctcc cccggaacaa gatcggtttc atgaaaagct tcccgcttga ccccgtaacc
    20041 gtcataccaa cgacactcaa ccataccgct ggagtatcca gtgacaatca tccgcgggcc
    20101 gccctcttta accgtaactt cctcactaac cataaagctc atactcgcct ccttttttcg
    20161 agtgaaactt gttcacctta gttgaagatg gcgaattttg cataatagcc agtgcgagta
    20221 ttaatgtgcc tgaccgcgca ttttcgctac tgctttcact cccagcacca cgatgccgcc
    20281 gatgataaat ccaagaatca gatttaaaac agtcggtaat atcatcgcca ccactgcact
    20341 ttgctgcccg gcgatatgtt caatagcgtg atgcagcggc gcaataccat gtaccacaat
    20401 cccgccgccg acgaggaaca tcgccagcgt gccgacaatc gataatgctt tcatcagcca
    20461 aggcgcgata atcaataacc ctttacctaa tgcctgcatc agcgcgctag atttttccgc
    20521 cagccaatac cctaggtcat caatcttaac gataacccct accagaccgt agacgcccac
    20581 ggtcactacc agcgcgatgc ctgaaagcac cagcacctga ttaagcaacg gcgcctctgc
    20641 cacaatcccc agcgtgatgg cgacgatttc cgcagacaaa ataaaatcgg tacgaatcgc
    20701 ccctttaatt ttgtcctttt caaacttcag cggatcctgc gccgccagct tctccagacg
    20761 ctgctggctc tgcgctggat cttctttatg tttacgcgcc tccagcatat gcagcacttt
    20821 ctctacccct tcaaagcaga gaaacgcgcc accaatcatc aacagtggcg taatcgccca
    20881 cgggataaac gcactgatga tcagcgccag cggcaccaga atcactttat tgatcagcga
    20941 tcctttcgcc acgccccaga ccacgggaag ttcccggttg gcccgcacgc ctgaaacttg
    21001 ttgcgcattg agcgataaat catcccctaa tacaccggcg gttttcttcg ccgccagttt
    21061 gcccatcaca gagatatcgt ccagcagtgt ggcgatatca tcgagcaacg tcagtaaact
    21121 actcccggcc aaaagcattc cctctcattt ttattgttga agggtgtaag tatgaagcaa
    21181 aaacggcagc tctgatactt gcttcacagg tcaacattta tttaacaatc aagtgcaatt
    21241 atatctctcg ccagcagaat gattttcacg tttactatga accacctttt tatttccgcc
    21301 gtgagggatt atgcgtttcc gccagttgtt accgcttttt ggcgcgctgt ttgcgttgta
    21361 tatcatttgg ggctcaacct attttgtcat tcggattggc gtgggaagct ggcctccgtt
    21421 aatgatggcg ggcgttcgat tccaggcagc cggtatttta ttgctggcat ttttgctact
    21481 gcgcggacac aaacttcccc cgctacgtcc gctgctcaat gccgcgctga ttggcctgtt
    21541 attgctggct gtcggtaatg gcatggtgac ggttgccgaa catcaaaatg ttccttccgg
    21601 catcgccgcc gtagtgattg caaccgtgcc cctctttacc ctgtgcttca gtcgcctgtt
    21661 tggcattaaa acgcgcaaac tggaatgggt gggtattgcc attgggcttg ccggaatcat
    21721 catgctcaat agcggtggaa atttaagcgg caatccgtgg ggcgcgattc tgattttaat
    21781 cggctcgatt agctgggcgt ttggctcagt ttatggctcg cgcattacct tacctgtagg
    21841 gatgatggcg ggtgcgattg agatgctggc ggcaggcgtg gtgttaatga tcgcatcgat
    21901 gattgcgggt gaaaaactga cggcgctccc ttccctgtca ggcttccttg cggtcggcta
    21961 tctggcgctg tttggttcga ttatcgccat taacgcttat atgtatttaa tccgtaatgt
    22021 cagtccggct ctcgccacca gctacgccta cgttaacccg gtggtcgcgg tcttgctggg
    22081 tacgggactg ggtggcgaaa cgctgtcgaa gattgaatgg ctggcgctcg gcgtaattgt
    22141 tttcgcggtg gtactggtca cgttgggaaa atatctcttc ccggccaaac ccgttgtcgc
    22201 gccagttatt caggatgcat caagcgagta aatgaatccc ctgcgtgtcg atctgcgcgc
    22261 tggcaccttc gccgcagatc cactcttcca gacgctcggt aagcgccgca tccgtcagct
    22321 tctcgcgcct acgtaacgca cactcccaga caatcagtac gcgccagccg agctcctgca
    22381 agcgactgat atcgcggcga tcgcgttcaa catttttacc tatcttctcc agccagaact
    22441 cagttcgagt cgcaggcact ttaaacagat agcagtgatg atgatgccag aagcagccgt
    22501 gggtaaatat cacgcagcga tattcatcaa cgacaaaatc cgggcgtccg ggcagactgg
    22561 cgtcctgaac gcgaaacgca aggccctgcc cggttaacaa actggcgagg cgcttctcaa
    22621 tcgccgtatc acgcgtggca atcgcgcgca tatttttgct gcgggtggcc ttatcgtgaa
    22681 cgtcgaccat gttgtgcctc ttgctgacgc aacgccaccg cctgtttgat ttttggctca
    22741 agcagttttg ccaccgcggc aaagaccggc acgaccaccg agttgccgaa ctggcgatag
    22801 gcctgagtgt ccgaaaccgg aatgcggaat ttcgcttctc ccggcgcttc aaaacccatt
    22861 aagcgcgcgc attcacgagg cgttaaccga cgtggacgat gttgctgatt cagcggatcg
    22921 tcaaagtctt tctcacccgt ggccatatcc caaccgcgat cgattaaaat ttccgcgcca
    22981 tctttgtaat aacgagcaga cagcgtacgg gtgacgcttt gcggattgtt cggataaacc
    23041 attccataac cgaagccgtt accgcgcgcc tgatgttttt tcgcatatcg atagaggtac
    23101 ttccacagca ccggcgtcag gatatatttc gcctcaacca tcgggtccag cagctgcgcc
    23161 agcgtcactc gctgtgcagg gaaacattcg ctgatatcac gcagagtaaa atcggctttc
    23221 agattcagat cgcgacgaaa acccaccagc acgatgcgtt cacggtgctg cggcaggaaa
    23281 tgtttaccat cgatgatttt cggatcgtcc ggcccgttat cttctgcatc ggcaacgtca
    23341 tagcccagtt cgtccagcgt ctgcatgatg atgcggaacg tttttccctt atcgtgactc
    23401 ttcaggtttt tgacgttttc gagcacaaac atcgccggac gacgcgcgtc gataatgcgc
    23461 accacatcaa agaacagcgt gccctgggta tcgcaggcaa aaccgtgcgc ccgcccgagc
    23521 gagttctttt tcgatacgcc agccagcgaa aatggctgac aagggaaacc ggccagtaaa
    23581 acatcgtgtt caggaatgtg ttgacgaata tgttccgccg ccgcctcatc actcacgcct
    23641 tcttgatgac tgagggtgat gtcgcggata tcttcattaa aatgatgcgt cgccgggtcg
    23701 caataatggt tggctttata agtgcgtacc gcatgtttgt tccattcgct ggtaaacacg
    23761 cactgtccac caatcgattc aaaaccacga cggatgccgc caattccggc aaatagatcg
    23821 ataaagcgaa acgcataatg cggatgatgt tccggtggtt tgggtaacaa cgtttgcaga
    23881 tgggcaaact ctttctcact taaacggtgc catgccgagt cattcgccag cgcacgtttt
    23941 aaaattgccg cgctccagtg attctcaccc acaccattaa gctgcgccac cagcatttta
    24001 acatcataaa tttgcagcag tttctccagc attgcctgtg cggcattccc ggtgctgtat
    24061 gaatcggtta ctgatatatt ttcctgcata gatttaaccg gccatctgag atggccgaca
    24121 ggttaacata attactgccg tttagggcca cgcgctcagc cagcgggtga aaacgcatct
    24181 atcaccttgt gatcgatacc ttcattctcc cccgccagtt cggcaccgag cttcgccatc
    24241 aaacagcgcc aagctcaatg cccctgaaac ggcaaatacg cgcgccaggc caatcgcccc
    24301 cagggcttcc agccgatccg catcctggac gattttagct tccgttgtta agggggcgat
    24361 ttgcgcgctg aaactgtgag cggcaatggc atgacaaacg gcctcaattt tctccgccgg
    24421 aaattgcacg aactcttcac ggagcaggcg acgcgtctct tctgccgcca ggattgaaga
    24481 acgctgccgt tgcggatgat ttttcgccag gctcacgata tcgtgaaaat aacaggcggt
    24541 taaaatcacc agcatatcga catcgtcatc tgccgccagt ttctgtgccg tagcccagac
    24601 acggcgaaaa tgacacaggt catgcgccgc gtcctgatgt tgatgatggt ttttcaacca
    24661 gttctcgaac tgtgcctgcc agtgttgtaa ctccatacgc gctccgttgc caaagagtcg
    24721 caaccttagc agtttttatt catagcgtct gcaacgtgcg ggacgaggtc gggtgtacca
    24781 tgcaatgccg cctaacaagg caccaaccag ggccataaac agaaatccga ccaatgctcc
    24841 taaccacttc cccgcggtag atcctaaatc actttcaaat cccgacacgg gggcatcggg
    24901 gatccggctc atcacccaga tgtatcgcgc cattgcccag acgaaatagc cacaccatgt
    24961 atagaaagcc c
//
LOCUS       DAJTQY010000057        24590 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.057, whole genome shotgun sequence.
ACCESSION   DAJTQY010000057 DAJTQY010000000
VERSION     DAJTQY010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24590
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.057"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(1..374)
                     /locus_tag="OO349_002406"
                     /pseudo
     CDS             complement(1..374)
                     /locus_tag="OO349_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            898..2826
                     /gene="thrS"
                     /locus_tag="OO349_002407"
     CDS             898..2826
                     /gene="thrS"
                     /locus_tag="OO349_002407"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HCR5867443.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2830..3372
                     /gene="infC"
                     /locus_tag="OO349_002408"
     CDS             2830..3372
                     /gene="infC"
                     /locus_tag="OO349_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HCR5867444.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPST
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3469..3666
                     /gene="rpmI"
                     /locus_tag="OO349_002409"
     CDS             3469..3666
                     /gene="rpmI"
                     /locus_tag="OO349_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HCR5867445.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3719..4075
                     /gene="rplT"
                     /locus_tag="OO349_002410"
     CDS             3719..4075
                     /gene="rplT"
                     /locus_tag="OO349_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HCR5867446.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTTLVEKAKAALA"
     misc_feature    4192..4316
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            4198..4242
                     /gene="pheM"
                     /locus_tag="OO349_002411"
     CDS             4198..4242
                     /gene="pheM"
                     /locus_tag="OO349_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HCR5867447.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4525..5508
                     /gene="pheS"
                     /locus_tag="OO349_002412"
     CDS             4525..5508
                     /gene="pheS"
                     /locus_tag="OO349_002412"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HCR5867448.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5523..7910
                     /gene="pheT"
                     /locus_tag="OO349_002413"
     CDS             5523..7910
                     /gene="pheT"
                     /locus_tag="OO349_002413"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HCR5867449.1"
                     /translation="MKFSELWLREWVNPAIDSDTLANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPVLQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLASPISVEMSAMRLSLWTGLLATVVYNQHRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            7915..8214
                     /gene="ihfA"
                     /locus_tag="OO349_002414"
     CDS             7915..8214
                     /gene="ihfA"
                     /locus_tag="OO349_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HCR5867450.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            8315..9295
                     /gene="btuC"
                     /locus_tag="OO349_002415"
     CDS             8315..9295
                     /gene="btuC"
                     /locus_tag="OO349_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HCR5867451.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPMWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            9358..9909
                     /gene="btuE"
                     /locus_tag="OO349_002416"
     CDS             9358..9909
                     /gene="btuE"
                     /locus_tag="OO349_002416"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HCR5867452.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGKVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            9909..10658
                     /gene="btuD"
                     /locus_tag="OO349_002417"
     CDS             9909..10658
                     /gene="btuD"
                     /locus_tag="OO349_002417"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HCR5867453.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     QMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10736..11161
                     /locus_tag="OO349_002418"
     CDS             10736..11161
                     /locus_tag="OO349_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCR5867454.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRDRFDLHLPRETKQQASIGTQIDKDELLPGDLVFFKTGSGQNGLH
                     VGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            11408..12121
                     /gene="ydiV"
                     /locus_tag="OO349_002419"
     CDS             11408..12121
                     /gene="ydiV"
                     /locus_tag="OO349_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HCR5867455.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQLLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLDRDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRDLLSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLEKGFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTPEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            12185..13621
                     /locus_tag="OO349_002420"
     CDS             12185..13621
                     /locus_tag="OO349_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HCR5867456.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNAAGVWGGETLLPGMSPLAQVYSGHQFVVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLREEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMMELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(13625..13816)
                     /gene="hemP"
                     /locus_tag="OO349_002421"
     CDS             complement(13625..13816)
                     /gene="hemP"
                     /locus_tag="OO349_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HCR5867457.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(13948..14994)
                     /gene="aroH"
                     /locus_tag="OO349_002422"
     CDS             complement(13948..14994)
                     /gene="aroH"
                     /locus_tag="OO349_002422"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HCR5867458.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAIEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTEIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLAFAVDTRF"
     gene            complement(15151..15984)
                     /gene="ppsR"
                     /locus_tag="OO349_002423"
     CDS             complement(15151..15984)
                     /gene="ppsR"
                     /locus_tag="OO349_002423"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HCR5867459.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            16316..18694
                     /gene="ppsA"
                     /locus_tag="OO349_002424"
     CDS             16316..18694
                     /gene="ppsA"
                     /locus_tag="OO349_002424"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HCR5867460.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPSEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(18751..20397)
                     /gene="fadK"
                     /locus_tag="OO349_002425"
     CDS             complement(18751..20397)
                     /gene="fadK"
                     /locus_tag="OO349_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HCR5867461.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNIPLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWLDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNLLEKQPADLSALRFFLCGGTTIPKKVARECQQRSIKLLSVY
                     GSTESSPHAVVNLDNSLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEMAAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASDKIQKFLLRKDIIRRLTQDVCEEIE"
     gene            complement(20454..20747)
                     /locus_tag="OO349_002426"
     CDS             complement(20454..20747)
                     /locus_tag="OO349_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCR5867462.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILTENPDINEFRKLMKA
                     CPAGLYKQDDVGNIHFDPAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(20744..22033)
                     /locus_tag="OO349_002427"
     CDS             complement(20744..22033)
                     /locus_tag="OO349_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR5867463.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNEGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSANSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(22089..23027)
                     /locus_tag="OO349_002428"
                     /pseudo
     CDS             complement(22089..23027)
                     /locus_tag="OO349_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707415.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
     gene            complement(23047..23811)
                     /locus_tag="OO349_002429"
     CDS             complement(23047..23811)
                     /locus_tag="OO349_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HCR5867464.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAIEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
     gene            24127..>24590
                     /locus_tag="OO349_002430"
     CDS             24127..>24590
                     /locus_tag="OO349_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HCR5867465.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFA"
BASE COUNT         6090 a         5938 c         6463 g         6099 t
ORIGIN      
        1 ctgttcaata taatccagta tgcttcgcaa atggttagct ttaataggcg tggtttccag
       61 taataaggaa atgatttctt ttttatagtg taatgtaatg agtatatttt ctgcatggat
      121 gggtgaagtg gcaaagttaa tggtaaaaag ggcagtggaa taacaatttt gtgatatttt
      181 cgacagtttg cggtaaattc ctgagtaaca cagtatgaca gcatcgaact taagtcagag
      241 gtatcaagta gggtgatgag ctggcgcgca aatttattaa taggctctga tgaaggtgtt
      301 ggtatgtcgc tatcgatgtg caatctatga ggagacttat aatgagttct gataatgata
      361 tcgttttgac tcataatcta ttcgccttat atatatctgg aggttttgag gatgatgatt
      421 aattattatc gccagaacga aatgtgcacg aaaaattgtt ataagaattg tgtcactttt
      481 atcattgtga tatgaccact tcgtcattaa tctcatgagc gtttaaaata ctaccgccaa
      541 atcacgcaac gaactttgct ggattcagtt tatgctgctg taaatccgct cgcagtaaac
      601 ctttcagacg caccgtgatg ttatcagtgg ttctttaagc gttttgctgg tgtactcact
      661 acaaacgaat tgcgaatcga atcaatgtga aacggaaagg tacaatctcc ctttttttag
      721 tgttaacatc gttcaaccgg gtggaaggtg agaggttaat gtctttcatt tgtgtacctg
      781 attgcgaacc aatttagcat ttgttggcta gatggtttcg caatgaactg ttaataaaca
      841 aatttttctt tgtatgtgat ctttcgtgtg ggtcaccact gcaaataagg atataaaatg
      901 cctgttataa ctcttcctga tggcagccaa cgccattacg accacgctgt aagccccatg
      961 gatgttgcgc tggacattgg tccaggtctg gcgaaagcct gtatcgcagg gcgcgttaat
     1021 ggcgaactgg ttgatgcttg cgatctgatt gaaaacgacg cacaactgtc gatcattacc
     1081 gccaaagacg aagaaggtct ggagatcatt cgtcactcct gtgcgcacct gttagggcat
     1141 gcgattaaac aactttggcc gcataccaaa atggcaatcg gcccggttat tgacaacggt
     1201 ttttattacg acgttgatct tgaccgcacg ttaacccagg aagatgtcga agcactcgag
     1261 aagcggatgc atgagcttgc tgagaaaaac tacgacgtca ttaagaagaa agtcagctgg
     1321 cacgaagcgc gtgaaacttt cgccaaccgt ggggagagct acaaagtttc cattcttgac
     1381 gaaaacatcg cccatgatga caagccaggt ctgtacttcc atgaagaata tgtcgatatg
     1441 tgccgcggtc cgcacgtacc gaacatgcgt ttctgccatc atttcaaact aatgaaaacg
     1501 gcaggggctt actggcgtgg cgacagcaac aacaaaatgt tgcaacgtat ttacggtacg
     1561 gcgtgggcag acaaaaaagc acttaacgct tacctgcagc gcctggaaga agccgcgaaa
     1621 cgcgaccacc gtaaaatcgg taaacagctc gacctgtacc atatgcagga agaagcgccg
     1681 ggtatggtat tctggcacaa cgacggctgg accatcttcc gtgaactgga agtgtttgtt
     1741 cgttctaaac tgaaagagta ccagtatcag gaagttaaag gtccgttcat gatggaccgt
     1801 gtcctgtggg aaaaaaccgg tcactgggac aactacaaag atgcaatgtt caccacatct
     1861 tctgagaacc gtgaatactg cattaagccg atgaactgcc cgggtcacgt acaaattttc
     1921 aaccaggggc tgaagtctta tcgcgatctg ccgctgcgta tggccgagtt tggtagctgc
     1981 caccgtaacg agccgtcagg ttcgctgcat ggcctgatgc gcgtgcgtgg atttacccag
     2041 gatgacgcgc atatcttctg tactgaagaa caaattcgcg atgaagttaa cggatgtatc
     2101 cgtttagtct atgatatgta cagcactttt ggcttcgaga agatcgtcgt caaactctcc
     2161 actcgtcctg aaaaacgtat tggcagcgac gaaatgtggg atcgtgctga ggcggacctg
     2221 gcggttgcgc tggaagaaaa caacatcccg tttgaatatc aactgggtga aggcgctttc
     2281 tacggtccga aaattgaatt taccctgtat gactgcctcg atcgtgcatg gcagtgcggt
     2341 acagtacagc tggacttctc cttgccgtct cgtctgagcg cctcctatgt gggcgaagac
     2401 aacgagcgta aggtaccggt aatgattcac cgcgcaattc tggggtcgat ggagcgtttc
     2461 atcggtatcc tgaccgaaga attcgctggt ttcttcccga cctggcttgc gccagttcag
     2521 gttgttatca tgaatattac cgattcacag tctgaatacg ttaacgaatt gacgcaaaaa
     2581 ctatcaaatg cgggcattcg tgttaaagca gacttgagaa atgagaagat tggctttaaa
     2641 atccgcgagc acactttgcg tcgcgtccca tatatgctgg tctgtggtga taaagaggtg
     2701 gaatcaggca aagttgccgt tcgcacccgc cgtggtaaag acctgggaag catggacgta
     2761 aatgaagtga tcgagaagct gcaacaagag attcgcagcc gcagtcttaa acaattggag
     2821 gaataaggta ttaaaggcgg aaaacgagtt caaacggcgc gccctaaccg tatcaatggc
     2881 gaaattcgcg cccaggaagt tcgcttaaca ggtctggaag gcgagcagct tggtattgtg
     2941 agtctgagag aagctctgga gaaagcagaa gaagccggag tagacttagt cgagatcagc
     3001 cctaacgccg agccgccggt ttgtcgtata atggattacg gcaaattcct ctatgaaaag
     3061 agcaagtctt ctaaggaaca gaagaaaaag caaaaagtta tccaggttaa ggaaattaaa
     3121 ttccgtccta gtacagatga aggcgactat caggtaaaac tccgcagcct gattcgcttt
     3181 ctcgaagagg gtgataaagc caaaatcacg ctgcgtttcc gcggtcgtga gatggcgcac
     3241 cagcaaatcg gtatggaagt gcttaatcgc gtgaaagacg atttgcaaga actggcggta
     3301 gtcgaatcct tcccaacgaa gatcgaaggc cgccagatga tcatggtgct cgctcctaag
     3361 aagaaacagt aaggccttca agtagcacta tctgtggagc ctttgggctt cacaggcatt
     3421 gttcgccttt gtttcgttta ttaacaatgc gaagtggaag ttattaaaat gccaaaaatt
     3481 aagaccgtac gcggtgctgc taagcgcttc aaaaaaaccg gtaaaggtgg ttttaagcac
     3541 aagcacgcta acctgcgtca cattctgact aaaaaagcga ccaaacgtaa acgtcacctg
     3601 cgtccgaaag ccatggtttc caaaggcgat ctgggcctgg taatcgcgtg cctgccgtac
     3661 gcataagccg ttaacgtttt taacttttta attagaatat agatacagga gagcacatat
     3721 ggctcgcgta aaacgtggtg ttattgcacg cgcacgtcac aagaaaattt tgaaacaagc
     3781 taaaggctac tacggtgcgc gttctcgcgt ataccgcgtt gccttccagg ctgttatcaa
     3841 agctggtcag tatgcttacc gtgaccgtcg tcaacgtaag cgtcagttcc gtcaactgtg
     3901 gattgcgcgt atcaacgcag cagcacgtca gaacggtatt tcttacagca aattcatcaa
     3961 tggcctgaaa aaagcctctg ttgaaatcga ccgtaagatc ctggctgata tcgcagtatt
     4021 cgacaaagta gcgttcacca ctctggttga aaaagcgaaa gcagctctgg cataagccag
     4081 ttgaaagagg gagctagtct ccctcttttc gtttcaacgc catcaaaaca ttgactttta
     4141 tcgccgtagc cttttcaata aaggtctttt gaagagtacc aaaaggtaac gcaagcaatg
     4201 aatgctgcta ttttccgctt ctttttttac tttagcacct gaatccagga ggctagcgcg
     4261 tgagaagaga aacggaaaac agcgcctgaa agcctcccag tggaggcttt ttttgtatgc
     4321 gcgttttgaa aaataaaaag ccagtctatc aggcgtaatt gtcgcagcca gtctggacac
     4381 ggccagtgcg gagaaaccgg agcgtactta agtacgtgag gatttcgagc actgcccggg
     4441 accaaaatgg caagtaaaat agcctgatgg gataggctct aagtccaacg aaccagtgtc
     4501 accactgaca caatgaggaa aaccatgtca catctcgcag aactggttgc cagtgcgaag
     4561 gcggccatta gccaggcgtc agatgttgcc gcgttagata atgtgcgcgt cgaatatttg
     4621 ggtaaaaaag ggcacttaac ccttcagatg acgaccctgc gtgagctgcc gccagaagag
     4681 cgtccggcag ctggtgcggt tatcaacgaa gcgaaagagc aggttcagca ggcgctgaat
     4741 gcgcgtaaag cggaactgga aagcgctgca ctgaatgcgc gtctggcggc ggaaacgatt
     4801 gatgtctctc tgccaggtcg tcgcattgaa aacggcggtc tgcatccggt tacccgtacc
     4861 atcgaccgta tcgaaagttt cttcggtgag cttggcttta ccgtggcaac cgggccggaa
     4921 atcgaagacg attatcataa cttcgatgct ctgaacattc ctggtcacca cccggcgcgc
     4981 gctgaccacg acactttctg gtttgacgct acccgcctgc tgcgtaccca gacctctggc
     5041 gtacagatcc gcaccatgaa agcccagcag ccaccgattc gtatcatcgc gcctggccgt
     5101 gtttatcgta acgactacga ccagactcac acgccgatgt tccatcagat ggaaggtctg
     5161 attgttgata ccaacatcag ctttaccaac ctgaaaggca cgctgcacga cttcctgcgt
     5221 aacttctttg aggaagattt gcagattcgc ttccgtcctt cctacttccc gtttaccgag
     5281 ccttctgcag aagttgacgt catgggtaaa aacggtaaat ggctggaagt gctgggctgc
     5341 gggatggtgc atccgaacgt gctgcgtaac gttggcatcg acccggaagt ttactctggt
     5401 ttcgccttcg ggatggggat ggagcgtctg actatgttgc gttacggcgt caccgacctg
     5461 cgttcattct tcgaaaacga tctgcgtttc ctcaaacagt ttaaataagg caggaataga
     5521 ttatgaaatt cagtgaactg tggttacgcg aatgggtgaa cccggcgatt gatagcgata
     5581 cgctggcgaa tcaaatcact atggcgggcc tggaagttga cggtgtagaa ccggttgccg
     5641 gtagcttcca cggcgtggtc gttggtgaag tggttgagtg tgcgcagcat ccgaacgctg
     5701 acaaactgcg tgtgacaaaa gtgaatgtcg gcggcgatcg cctgctggac atcgtctgcg
     5761 gtgcgccaaa ctgccgtcag ggcctgcgtg tggcggtagc taccattggt gctgttctgc
     5821 cgggtgattt caaaattaaa gcggcgaaac tgcgtggcga accgtctgaa gggatgctgt
     5881 gctccttttc tgagctggga atttctgacg atcacagtgg cattatcgaa ctgcctgcgg
     5941 atgcgccgat tggcaccgac atccgcgaat acctgaaact cgatgataac accatcgaaa
     6001 tcagcgtgac accaaaccgt gccgactgct taggtatcat tggtgttgcg cgtgacgttg
     6061 ccgtgctgaa tcagctgccg ctggttgaac cggaaatcgt tccggttggt gcgaccatcg
     6121 acgacacgtt gccgattaca gtcgaagcgc cggaagcctg cccgcgctat cttggccgtg
     6181 tggtaaaagg cattaacgtt aaagcgccaa ctccgctgtg gatgaaagaa aaactgcgtc
     6241 gttgcgggat ccgttctatc gatgcagttg ttgacgtcac caactatgta ctgctcgaac
     6301 tgggccagcc gatgcacgct ttcgataaag atcgcattga aggcggcatt gtggtgcgga
     6361 tggcaaaaga gggcgaaacg ctggtgctgc ttgatggcac tgaagcgaag ctgaatgctg
     6421 acactctggt catcgccgac cacaacaagg cgctggcgat gggcggcatc tttggtggcg
     6481 aacactctgg cgtgaatgac gaaacgcaaa acgtcttgct ggaatgcgct ttcttcagcc
     6541 cgctgtctat caccggtcgt gctcgtcgtc atggcctgca tactgatgcg tctcaccgtt
     6601 atgagcgtgg cgttgatccg gtactgcagc acaaagcgat ggaacgtgcg acccgtctgc
     6661 tgatcgacat ctgcggtggt gaggctggcc cggtaattga tatcaccaac gaagcaacgc
     6721 tgccgaagcg tgcaaccatc actctacgtc gtagcaaact ggatcgcctg ataggccatc
     6781 atattgctga tgagcaggta actgacattc tgcgtcgtct cggctgcgaa gtgaccgaag
     6841 gcaaagacga atggcaggca gttgcgccga gctggcgttt cgatatggag attgaagaag
     6901 atctggtcga agaagtcgcg cgtgtttacg gctacaacaa catcccggat gagccggtac
     6961 aggcaagcct gattatgggg actcaccgtg aagctgacct gtcgctcaag cgcgtgaaaa
     7021 cgctgctcaa cgacaaaggc tatcaggaag tgatcaccta cagcttcgtt gatccgaaag
     7081 tgcagcagat gatccatcca ggcgttgaag ccttactgct ggcaagcccg atctctgttg
     7141 aaatgtcagc aatgcgtctt tctctgtgga ccggcctgct ggcaaccgtg gtgtacaacc
     7201 agcaccgtca gcagaaccgt gtgcgcattt tcgaaagcgg tctgcgtttc gtaccagata
     7261 ctcaggcacc gttgggcatt cgtcaggatc tgatgttagc cggtgtgatt tgcggtaacc
     7321 gttacgaaga gcactggaac ctggcaaaag agaccgttga tttctatgat ttgaaaggcg
     7381 accttgaatc cgttctcgac ctgaccggta aactgaatga ggttgagttc cgtgcagaag
     7441 cgaatccggc actgcatccg gggcaatccg cagcgattta tctgaaaggt gaacgtattg
     7501 gttttgttgg agttgttcat cctgaactgg aacgtaaact ggatcttaac ggtcgcactc
     7561 tggtgttcga actggagtgg aacaagctcg cagaccgcgt ggtgcctcag gcgcgcgaga
     7621 tttctcgctt cccggcgaac cgtcgtgaca tcgcggtggt ggtcgcagaa aacgttcccg
     7681 cagcggatat tttatccgaa tgtaagaaag ttggcgtaaa tcaggtagtt ggcgtaaact
     7741 tatttgacgt gtaccgcggt aagggtgttg cggaggggta taagagcctc gccataagcc
     7801 tgatcctgca agataccagc cgtacactcg aagaagagga gattgccgct accgtcgcca
     7861 aatgtgtaga ggcattaaaa gagcgattcc aggcatcatt gagggattga acctatggcg
     7921 cttacaaaag ctgaaatgtc agaatatctg tttgataagc ttgggcttag caagcgggat
     7981 gccaaagaac tggttgaact gtttttcgaa gagatccgtc gcgctctgga aaacggcgaa
     8041 caggtgaaac tctctggttt tggtaacttc gatctgcgtg ataagaatca acgcccggga
     8101 cgtaacccga aaacgggcga ggatattccc attacagcac ggcgcgtggt gaccttcaga
     8161 cccgggcaga agttaaaaag ccgggtcgaa aacgcttcgc ccaaagacga gtaatctgat
     8221 ctaactaaaa aggccgctat gcggcctttt ttcttttcac tgtcgaagag tcaccgtaaa
     8281 atcaacgcca tgacacttca gcagaacgga taccatgctg acacttgccc gccaacaaca
     8341 gcgacaaaat attcgctggt tattatgcct gtcagttttg atgctgctgg cgcttctctt
     8401 aagcctttgc gccggtgaac aatggatttc gccaggtgac tggtttactc ctcgtggcga
     8461 actgttcgtc tggcaaattc gcctgccacg tacgctggct gtattgctgg ttggtgcggc
     8521 gctggctata tccggcgctg taatgcaggc gttgtttgaa aatcctctgg cagaacctgg
     8581 actacttggc gtctctaacg gcgcaggcgt ggggcttatc gccgcggtat tgcttgggca
     8641 agggcaactc cccaactggg cgctagggct gtgtgcgatt gctggcgcgc ttatcatcac
     8701 tttaatactc ttacgtttcg cccgtcgtca tctttcgacc agtcggttat tgctggctgg
     8761 cgttgcatta gggattatct gcagcgcact aatgacgtgg gctatctact tttccacctc
     8821 tgttgattta cgtcagctga tgtactggat gatgggcggt tttggcggcg tagactggcg
     8881 gcaaagctgg ctgatgctgg cattgatccc cgtgttgttg tggatctgtt gtcagtccag
     8941 gccgatgaat atgttagcac ttggcgagat ctcggcgcgg caactgggtt tacccatgtg
     9001 gttctggcgc aatgtgctgg tggcagcgac cggctggatg gttggcgtca gtgtggcgct
     9061 ggcgggtgct atcggcttta ttggtctggt gatcccacat attcttcggt tgtgtggttt
     9121 aaccgatcat cgcgtattac ttcccggctg cgcgctggca ggggcgagcg cattgctgct
     9181 ggccgatatt gtagcgcgcc tggcattagc tgccgcagag ctgcctattg gcgtggtcac
     9241 cgcaacgttg ggtgcgccgg tgtttatctg gttattgtta aaagcaggac gttagccgca
     9301 aaaagacggt ctatgattaa aagctaagat ttttactgac cacacctagg agaaacgatg
     9361 caagattcca ttctgacgac cgtagtgaaa gatatcgacg gtgaagtgac cacgctggag
     9421 aagtacgccg gtaatgtgct gttaattgtc aatgtcgcct caaagtgtgg cttaacgccg
     9481 caatatgagc agttggagaa tattcaaaaa gcctgggccg atcgaggttt tgtggtgctg
     9541 ggattcccgt gcaaccagtt tctggaacaa gaaccgggca gcgatgaaga gattaaaact
     9601 tactgtacca ccacatgggg ggtgacgttc ccgatgttca gtaagattga agttaatggc
     9661 gaaggacgcc atccgctgta tcaaaaattg attgccgcag cgccgaccgc agtcgcgccg
     9721 gaagagagcg gattctatgc ccgtatggtc agcaaaggcc gtgcaccgct gtacccggat
     9781 gatattttat ggaattttga aaaattcctg gttggcaggg acggaaaagt catccagcgt
     9841 ttttccccgg atatgacgcc ggaagatccc attgtgatgg aaagcattaa actggcgttg
     9901 gcaaaataat gtctattgtg atgcagttac aggatgttgc ggaatctacc cgcctggggc
     9961 cgctttcagg cgaggttcgg gctggggaaa tcctgcactt ggtggggccg aatggcgcgg
    10021 gtaagagtac cttactggcg caaatggccg gaatgaccag cggtaaggga agcattcagt
    10081 tcgcggggca accactggaa gcatggtccg caacaaaact cgcgctgcat cgcgcctatc
    10141 tttcacaaca gcagacgccg ccgtttgcaa tgccggtctg gcactacctg acactgcatc
    10201 agcacgataa aacgcgtacc gaactactga atgatgtcgc aggggcgctg gctcttgatg
    10261 acaaactcgg tcgtagcacc aatcaacttt ccggcggtga atggcaacgc gtacgtcttg
    10321 ctgcggtggt gttgcaaatc acaccacaag ccaatcccgc aggccaattg ctgcttcttg
    10381 atgagccgat gaacagtctt gatgttgcgc aacaaagtgc gttagacaaa attctgagcg
    10441 cgctgtgtca gcaaggactg gcgattgtga tgagcagtca cgatctcaac cacacgttgc
    10501 gtcatgcgca tcgggcgtgg ttgctaaaag gtggaaaaat gctggccagt ggacgcagag
    10561 aagaggtgct cacgccgcca aatctggcgc aggcctatgg gatgaatttt cgccgtctgg
    10621 atatcgaagg tcacagaatg ctgatttcga ccatctgatt tcattagtat cccgaaaatc
    10681 gggtaattac gacgctaaat taatgcaaga ataaaaaaca gaggaaccgt acggaatgcg
    10741 tttctgcctt attttgatca cagcactgtt tctggccggg tgtagccacc ataaagcacc
    10801 gccgccaaat gccagacttt ctgattcgat taccgttatt gccggtttga acgaccagct
    10861 acaaagctgg catggcacgc cgtatcgtta tggtggcatg acgcggcgcg atcgtttcga
    10921 tttgcatctg ccccgtgaaa ccaaacaaca agcctctatt ggcacgcaaa ttgataaaga
    10981 cgagttgctg cctggtgacc tggtcttttt caaaacgggt tccggacaaa atggtttgca
    11041 tgtaggtatt tacgatacca acaaccaatt tatccacgcc tctaccagca agggagtgat
    11101 gcgttcctca cttgataatg tctattggca gaaaaatttc tggcaggcga gacgaatcta
    11161 gtatgtcgaa aaattgacgg cgatgataca tagccgtcaa tttactcctg aatacagtag
    11221 tattttgcag acaaaaattc cgtaaatgga ttattcgttt ataatcagga taatctaaaa
    11281 aaaggccccc tgaaaaagtg gttaacatgg aacaaatgaa agttactcct tctgtaattg
    11341 ttatgtctta tggcctcatc ccttttagcc ggatactgaa aaacatcctt cgagagggac
    11401 ggttaccatg aagatttttt tggagaatct ttatcattct gattgctatt tcctgccgat
    11461 aagagataac cagcaactcc ttgtcggcgt tgagttaatt acccatttct ccagtgaaga
    11521 tggcacggtt cgtatcccga cgagtcgggt tatagcgcaa cttaccgagg agcaacattg
    11581 gcaattattt tcagagcagt tggaattact aaaatcatgc caacactttt ttattcaaca
    11641 taaactcttt gcctggctta atttaacgcc acaagtggct acattattat tagacaggga
    11701 taattacgca ggcgaactat taaaatatcc gtttattgag ctgttgatta atgagaatta
    11761 cccgcatctc aatgaaggaa aagataatcg cgatcttttg tccttatcgc aaatgtatcc
    11821 ccttgtgtta ggcaatttgg gagcaggtaa tagcacaatg aaagcggttt ttgatggtct
    11881 tttcacccgc gtaatgctgg agaaaggttt tattcagcaa caaatcacgc atcgctcctt
    11941 cgaaccgttt attcgtgcga tccaggcgca aatttcccct tgttgcaatt gcatcatcgc
    12001 ggggggcatt gatacgccag aaatattggc acagattacc cccttcgatt tccatgctct
    12061 ccagggatgc ctgtggcctg ccgttcctat aaatcagata acgacgttgg ttcagcgata
    12121 accctcctgt ttgctggtgt ttaagacgag agtaaccgtc tacactatca aacagatagg
    12181 atctatgacc ctgtctttta ttacccgctg gcgcgatgaa ttgccagaaa cctatacagc
    12241 actttcccct acgcctttaa ataatgcccg gctgatttgg cataataccg aactcgctaa
    12301 cacgctgagt attccatcgt cgctgtttaa aaatgccgca ggtgtctggg gcggcgaaac
    12361 cctactgcct ggtatgtcac cactggcaca ggtttacagt ggtcatcagt tcgtcgtctg
    12421 ggcgggccaa ctgggtgatg ggcgcggcat tttacttggc gaacaactgc ttgctgatgg
    12481 cactacaatg gactggcatc tgaaaggtgc tggcctgacg ccttattcgc gaatgggtga
    12541 tggacgggcg gttttacgtt cgacgatacg agaaagtctc gccagtgagg cgatgcatta
    12601 tctgggcatt ccgacgaccc gcgcgttaag tatcgtcacc agcgattcgc cagtgtatcg
    12661 ggaaacggtg gagccaggcg cgatgctgat gcgtgtggca ccaagtcatc tgcgctttgg
    12721 tcatttcgaa catttttact atcgccgcga gccggaaaag gttcgtcagt tggctgactt
    12781 tgccattcgt cattactggt cacatcttga agacgatgag gacaaatacc gtctctggtt
    12841 taacgatgtt gtcgcacgta ccgcgtcgtt aattgcccaa tggcagacgg tcggctttgc
    12901 acatggggtg atgaataccg acaacatgtc gctgctggga ctgacgcttg attacgggcc
    12961 gtttggtttt cttgatgatt acgaacccgg ttttatttgt aatcactcgg atcatcaagg
    13021 gcgttatagc tttgataatc aacctgctgt cgcgttgtgg aatttacagc gtctggcgca
    13081 gacattgtca ccatttgttg ccgtagatgc cctgaatgag gccctggaca gctatcagca
    13141 ggttttgttg acgcattatg gacaacggat gcggcagaaa ctgggcttca tgacggagca
    13201 aaaagaggat aacgcgctac tgaatgaatt attcagtctg atggcgcgag agcgcagcga
    13261 ttatacccgc acattccgca tgctgagtct gaccgagcag cacagcgcgg cgtcaccgct
    13321 acgtgaggag tttattgatc gtgcggcatt tgatgactgg tttgcccgtt atcgggggcg
    13381 tttgcaacaa gacgaggtta gcgatagtga gcgtcagcaa ctgatgcaaa gcgttaaccc
    13441 agctctggta ttgcgcaact ggttggcgca acgggcgatt gaagcggcag aaaagggtga
    13501 tatgatggaa ttgcaccgcc tgcatgaggc gttgcgaaat cctttcagcg acagagatga
    13561 tgactatgtc agtcgtccac ctgactgggg taaacggctg gaagtcagct gctcgagtta
    13621 aaggctactt agttaacagc agcttgccag cctgagtttt acggagcaga tattcttgcc
    13681 cgtcatgatc gataatcagt ttgccatccg gacctaacag tgtctggctg gtaattcgcc
    13741 ggatgggctg cggggaagcg gtcttgtgat tggcatccgt ttcaggcgtg agttttctgc
    13801 tatccgtata acgcataaaa attaataata attgatataa caatgatttc tattatcaat
    13861 attatggcag gccagagcaa gcagtaatgt gttgagaaga caacaagttg cgcagaaatg
    13921 cccatcccat caggaatggg cacgcattca gaagcgggta tctaccgcaa aggcgagttt
    13981 ttcgaccagg cgttcggtat cttcccagcc cagacacggg tcggtaatgg attgaccgta
    14041 agtgagcggc tgaccgccga cgattttttg cgttccttcg cgcaggaaac tttccgccat
    14101 aattccagca atctccgtag agccattgcg gatttgctga caaatatcct cacaaacttc
    14161 taactggcga cggtgctgct tctggcagtt accgtggctg aaatccacca ccagatgttc
    14221 aggtaaatca aactcgtgca gcgtatcgca ggctgcggcg atatcatcgg catgataatt
    14281 cggttttttg ccgccacgca taataatgtg gccatacggg ttgccgctgg tctgatagat
    14341 ggtcatctga ccatttttgt ctggcgagag gaacatatgg ctggcgcggg ctgcgcggat
    14401 agcatccacg gcaatccgcg tgttgccatc ggtaccattt ttaaaaccta ccggacagga
    14461 gagtgctgaa gccatttcgc ggtggatctg actttcggta gtacgagcgc caatcgcgcc
    14521 ccaactgatt aaatcagcaa taaactgacc ggttaccata tcgaggaact cagtcgcggt
    14581 agggacaccc agctcattta cctgtaaaag taatttgcgc gccagctcca gaccgtgatt
    14641 tacccgatag ctgccgttta aatccggatc ggagattagc cctttccagc cgacaacagt
    14701 tcgtggtttt tcaaaatagg tgcgcattac gatttccagc cgtgactggt actggttgcg
    14761 cagcgactgc agacgggtgg cgtactctat tgcagcggtg agatcgtgga tcgagcaggg
    14821 gccaataatg accaacagtc gcttatcttc accattcagt attttttcaa ttctgcggcg
    14881 ggagtcggtg acatgggtgg cgacgccagg cgttacggga taccgtagcg cgagttcggc
    14941 gggcgttacc aggctctcaa tacgcgcagt acggagttcg tcagttctgt tcattacaag
    15001 tctcagaaat ttgttgcttc tacggcagga ctaccggaaa gtgatgcgca tcacgataaa
    15061 ccaatcgcag ctgatttcaa gtgcgaggtg tgtcacacct cgccgggtgg ttagcatgat
    15121 aacaaaaaaa caaaccaatg cactagttct ctagtacatt cggcgactaa ggcccatgat
    15181 atcgaggatc ttggtggcaa tctcttctac cgaataattg gtactgttaa tccacgggat
    15241 ctgattttta cggtacaagg cttccacttc cgcgacttcc atcctgcact gacgaagcga
    15301 ggcatagcga ctgttctccc gacgttcctc gcgaatcgcc gccagacgtt ccgggtcgat
    15361 agtcaggccg aacaatttat gctgaagcgg tttgagcgac gcgggtagca ccagattatc
    15421 catatcgtcg gcaataaagg ggtagtttgc cgcgcggata ccaaattgca ttgccagata
    15481 cagactggtg ggggttttac cacagcgaga aacaccgagc aggatcacct gagcctgatc
    15541 cagattgcgc aacgaaatgc cgtcatcgtg ggcgagggtg taatcaatcg ccgcaatgcg
    15601 cgcatcatat ttattgagat tattagggtt aaggccatgg gtacgatgag caatcggcgt
    15661 tggatccagt ttcatctctt gttgtagcgg ggcaaccagc gcctgaacga tatcctggca
    15721 aaagccttca ctttgcaaga tgatggcgcg aatctccggc aacacgatgg agtagaagac
    15781 cagcgggcgc acgcctgtct ggtgataaat tgcgtcaatc tgatccttca ctgcccgtgc
    15841 acggctctca ttttcgacaa acggcagcgt gatgctgctg atagtgacgg gaaattgtga
    15901 cattactgcg tgtcctaata cctccgcagt tattgccgta ccatcagaaa tataaaaaac
    15961 gtggcgatca acagcattat ccattttgtt cttcccgtga agcagacata attctatgaa
    16021 agcataaatt aaaagcgcac agaagcgtag gacgtaatgt ctggtttata aaatgaacct
    16081 tcattttatt ttttataaaa acagcatttc atttttatgg tttcgtttat accgatggtt
    16141 tatgtggaaa ttgtcgaata gagcaggttt gcgcaacgct gggatcagtc ttaaaaagta
    16201 aaaaaatata tttgcttgaa cgattcaccg tttttttcat ccggttaaat atgcaaagat
    16261 aaatgcgcag aaatgtgttt ctcaaaccgt tcatttatca caaaaggatt gttcgatgtc
    16321 caacaatggc tcgtcaccgc tggtgctttg gtataaccaa ctcggcatga atgatgtaga
    16381 cagggttggg ggcaaaaatg cctccctggg tgaaatgatt actaatcttt ccggaatggg
    16441 tgtttccgtt ccgaatggtt tcgccacaac cgccgacgcg tttaaccagt ttctggacca
    16501 aagcggcgta aaccagcgca tttatgaact gctggataaa acggatattg acgatgttac
    16561 tcagcttgcg aaagcgggcg cgcaaatccg ccagtggatt atcgacactc ccttccagcc
    16621 tgagctggaa aacgccatac gcgaagccta tgcacagctt tccgccgatg acgaaaacgc
    16681 ctcttttgcg gtacgctcct ccgccaccgc agaagacatg ccggacgctt cctttgccgg
    16741 tcagcaggaa accttcctca acgttcaggg ttttgacgcc gttctcgtgg cagtgaaaca
    16801 tgtatttgct tctctgttta acgatcgcgc catctcttat cgtgtgcacc agggttacga
    16861 tcaccgtggc gtggcgctct ccgctggtgt tcagcggatg gtgcgctccg acctcgcatc
    16921 ttctggcgtg atgttctcca ttgataccga atccggcttt gaccaggtgg tgtttatcac
    16981 ttccgcatgg ggccttgggg aaatggtcgt gcagggtgcg gttaacccgg atgagtttta
    17041 cgtgcataaa ccgacactgg cggcgaatcg cccggctatc gtgcgccgca ccatggggtc
    17101 gaaaaaaatc cgcatggttt acgcgccgac ccaggagcac ggcaagcagg ttaaaatcga
    17161 agacgtaccg caggaacagc gtgacatctt ctcgctgacc aacgaagaag tgcaggaact
    17221 ggcaaaacag gccgtacaaa ttgagaaaca ctacggtcgc ccaatggata ttgagtgggc
    17281 gaaagatggc cacaccggca aactgttcat tgtgcaggcg cgtccggaaa ccgtgcgctc
    17341 acgcggtcag gtcatggagc gttatacgct gcattcacag ggtaagatta tcgccgaagg
    17401 ccgtgctatc ggtcatcgca tcggcgcggg tccggtgaaa gtcatccatg acatcagcga
    17461 aatgaaccgc atcgaacctg gcgacgtgct ggttactgac atgaccgacc cggactggga
    17521 accgatcatg aagaaagcat ctgccatcgt caccaaccgt ggcggtcgta cctgtcacgc
    17581 ggcgatcatc gctcgtgaac tgggcattcc ggcggtagtg ggctgtggag atgcaacaga
    17641 acggatgaaa gacggtgaga acgtcactgt ttcttgtgcc gaaggtgata ccggttacgt
    17701 ctatgcggag ttgctggaat ttagcgtgaa aagctccagc gtagaaacga tgccggatct
    17761 gccgttgaaa gtgatgatga acgtcggtaa cccggaccgt gctttcgact tcgcctgcct
    17821 gccgagcgaa ggcgtgggcc ttgcgcgtct ggaatttatc atcaaccgta tgattggcgt
    17881 ccacccacgc gcactgcttg agtttgacga tcaggaaccg cagttgcaaa acgaaatccg
    17941 cgagatgatg aaaggttttg attctccgcg tgaattttac gttggtcgtc tgactgaagg
    18001 gatcgcgacg ctgggtgccg cgttttatcc gaagcgcgtc attgtccgtc tctctgattt
    18061 taaatcgaac gaatatgcca acctggtcgg tggtgagcgt tacgagccag atgaagagaa
    18121 cccgatgctc ggcttccgtg gcgcgggccg ctatgtttcc gacagcttcc gcgactgctt
    18181 cgcactggag tgtgaagcag tgaaacgtgt gcgcaacgac atggggctga ccaacgttga
    18241 gatcatgatc ccgttcgtgc gtaccgtaga tcaggcgaaa gcggtggttg aagaactggc
    18301 gcgtcagggg ctgaaacgtg gcgagaacgg gctgaaaatc atcatgatgt gtgaaatccc
    18361 gtccaacgcc ttgctggccg agcagttcct cgaatatttc gacggcttct caattggctc
    18421 aaacgatatg acgcagctgg cgctcggtct ggaccgtgac tccggcgtgg tgtctgaatt
    18481 gttcgatgag cgcaacgatg cggtgaaagc actgctgtcg atggctatcc gtgccgcgaa
    18541 gaaacagggc aaatatgtcg ggatttgcgg tcagggtccg tccgaccacg aagactttgc
    18601 cgcatggttg atggaagagg ggatcgatag cctgtctctg aacccggaca ccgtggtgca
    18661 aacctggtta agcctggctg aactgaagaa ataaaataaa tccccggcgg cgttcagtcg
    18721 ccggggttat gtgatccccg aagatgaaac ttattcaatc tcttcacaga catcctgcgt
    18781 taatcgccgt ataatatctt ttcttaacaa aaacttttgt attttatctg aggcagtgcg
    18841 cggtagtttt tcgattacca cgatatgttc aggatattta tattttgcga cccgtttacg
    18901 gctaaaaaaa gccgccatct cttccagcga taatgaatga tgcggcgctt tcagcacgac
    18961 ataagcgcat gatcgttcac ctaaacgttc atcgggcatt gcaaccacac aggcatcgtg
    19021 aattttagga tgctgcaata aaatatcttc cacttcacgg ctgctaatat tttcgccgcc
    19081 gcggacaata atatcttttt tgcgtccggt aatttttata tagccagcct catccatacg
    19141 gcagagatcg ccgctgtaat accagccttc ttcatccagc gcacgggcgg ttaattcagg
    19201 ttcatcaaaa taccccataa acacattggg gccacgcgat gcttcttcac cttcgcaacc
    19261 tggcggtaag gtcttgcgtg cgtcatcgac cactttaatc tctacacctg cggcagcgta
    19321 accatcggta tgcataaagc gcgacaaaga attatcgaga ttcaccaccg catgcggcga
    19381 actttctgtg gaaccataga cacttaataa tttaatgctg cgctgctggc attcgcgcgc
    19441 cacttttttg gggattgtgg taccgccgca aagaaagaag cgcagcgctg aaaggtctgc
    19501 gggttgtttc tctagtaaat tcaaaagatc atagacaaac ggcgttgcgc ctagcataca
    19561 ggtgcaacgc tgctgctcaa gcagcgcgag acacgcatca ggagtgaaaa tatctaacaa
    19621 cacgctgcga gcgccaatta agaatggtgc cgttacgcca tgcagaaagc ccgttgcgtg
    19681 accaagtggc gcaggcatca taaagacatc cagccaggtc agattcagcc gcgcgcaata
    19741 agcccgctca ctggcgagaa tattgttatg cgttagcatc acgccctttg gcagaccctc
    19801 ggttccggag gtaaacagca ccgcagctaa ttcatcgccg tgggtcgtta tcgccgtggt
    19861 cagtgggata ttgtcggcga taatctgact taatgagagg gaagaggtgg cgggagccag
    19921 tttgtccacg ccgacaattt gttgtagttg tggaagctga ttttgcagcg gcaggattaa
    19981 atctaccgga cgcgtttgtt taaacaacgt cggtgcaaag aacatttttg cctgacactt
    20041 attgagcacc cacaccagtt ctgcttcccg ccaggaaggc aacagcggca cggaaaccgc
    20101 accgattttc aggcaggcaa gatagataac ggtaaattca caccagccag gcagttgaaa
    20161 tgcgatgcga tcgcctgatt caataccctt cgctaacatc cagtttgcca gacagctcgc
    20221 ggcgtgatcg agcgcgctat aggtgtacga tgcaccatga ttatcgacca cggcaatttt
    20281 gtctggcatc gcacgagcgg tctgctgcca gtaatcggcc agtgaagcat cgccccatag
    20341 cccttgctga cgatacgccg cacgacgttg ttcgttaaac gttaatgtca ctttcatttt
    20401 ggactctcga aacagaacat caggcccgag ttgcgggcct gtgggatgca tgattagccg
    20461 tagcgaaaat caataccgaa ggtgcctgcg ggatattgcc actgttcgag aatagtgtta
    20521 ccgcacagca cccgacaggt gccgcactcc agacaaccgg cgggatcaaa atgaatgttt
    20581 cctacgtcat cctgcttata aagtccggca gggcaggctt tcattaattt acggaattca
    20641 ttgatatcgg gattttccgt cagaatgata tgcgggtggc cctcatcaac atggaattta
    20701 ttgacgccta atttgatgtc gacgttaacc gtagcgttct ggctcatagc gcggttgctc
    20761 ccttaatgcc atctttcagc aagttgatca gcccaatttt cttcgcgtgt cccatgatca
    20821 ttttgcgtac cggctggttt ggtttgccgt caatggtgaa catctcgttc atgatgtcgg
    20881 ctaccattcg tgggtattgg ctaaacaggc gcgggttttc catcagcgcc gggatcttgc
    20941 gaaaatgctg catatcacgc atgacgcagc tttgttccag ctcacgtttg tattgcgcca
    21001 gactgttggc ggagaaatcc gcgcgttctt tggcggcgat caccgttgtg gcggcagcct
    21061 gagccgatgc aatggctaaa tccatgccgc ggaccgtaaa acccaaattc aggcagaagc
    21121 ctgcggcgtc accaacgatc atcacgccct cgttaaccag ttgcggcacc attgccagac
    21181 cgccttccgg caccatatgc gcggaatatt caagcagttt gccgccgcta atcagcgggc
    21241 gaatggcggg gtgttgttta aaatcttcca gcatttgcgg cacgcttttt tgcgcatggg
    21301 cgatatcacc caatccacaa accagcccca aggatatgga atccttgttg gtatagagaa
    21361 atcccccgcc catcaggccg tcagaagggg aaccggcaaa cagccaggcg gcaccttcct
    21421 cgcccgtaat attaaagcga tcgttgatct gttctggtgt gaggccaata acctctttaa
    21481 caccaacagc gtaatgatgc ggatcggaag cgggaaccat tcccagcgag cggccaagca
    21541 tcgagttaac gccatcagcc agaatcacca cattcgcttc gagaatatca tccccagcct
    21601 gcacgccagt gaccttgttt ccttcacgaa ccagcgcgtc gacgcgaacg cctgggataa
    21661 actgcgcgcc cgcctgctcg gcttgttcca tcaaccacgg gtccagacga ttacgcaata
    21721 cggtataaga tgcgtgttga ggaacatctg gttgctcgcg gtgaaaatcg agggtaacgg
    21781 cgctttcttc ggttaagaag gagattttct cgcgtgtgac cttgcgttct accggcgctg
    21841 atactgcaaa gcctggaatg attgcttcaa gtgtgtgggc ataaagacgc ccgccggtca
    21901 tgtttttaca tccggcactg tcgccgcgtt ctatcaccag gacatccagc ccggctcgtg
    21961 ccatgaccag tgcggcaacg ctaccagcaa cgcccgcacc gaccacaatg gcatcaaatt
    22021 tgtcatccga catgcgttat gctcccttcc tgctgcgttt acagcgggag atgtggttga
    22081 aaggggaatt agcggctcaa ctggctaatc agggcaggga cgactttgta gatatcgccc
    22141 accagaccgt agtcggcata gttgaagatt ggcgcatttt tatctttatt gatggcgaca
    22201 atcactttta cgccgttgcc gccaaccata tgttggatct gcccggagat ccccagcgtc
    22261 aggtagagat cggatttcag caacacgccg gagacaccga tataacgttc acgctccatc
    22321 cagttctcgc cttcggcaat tggacgtgaa cagccgactt cagcattcag caccgccgcc
    22381 agttcgtgga ccattttcag gtcatcctgc gccgccagac cacgaccgac gccaaccaca
    22441 cgttttgctt tgctcaggtc cacgctgctt gcggctttcg cacggcgttc gcgacagaga
    22501 atttcatgac gcggagcaac ataaggtacc gtttctgtcg ggcactgatg agaggtatca
    22561 ctggtgcacg gttcctgaac accgggtgca agggtaatga tcgccagcgg gctgttgatc
    22621 ttttcctaag cgaacgctaa cccgccatac atccggtgtt cggcgcaaat gtgaccatcg
    22681 acaatatcca ccgccgtggc atcgttcacc agcgccgcat tcagttgcac acttaaccgt
    22741 gctgccagag ctttaccacg tttcgtcgcg gccaacagca acatagcggg gtgtttatct
    22801 ttcagcaggg cagcaatgct ttcggcgtaa ttttcagtgc gttgcagcgc gtcgttttgc
    22861 tcaagaacat aaatacattt tggaccataa ggcataactg cctgcgcctg gtcggtattt
    22921 tgtacaatgg catacacctg ttggccccat tgctgagcgc cgccaaacag ttcagcataa
    22981 cgttcaggat tatcgctaaa gacccagacg ctgtttaatt gactcatcgt ttcttctccg
    23041 taggcttcag ttcagggctt tcttcagatg ttccgccagc tcggcaatgg cctccggcga
    23101 atcgttatcg agaatgatgt gcttacgttc tgtttgcggc ggtacgcgaa tgccgaccag
    23161 ttcagcaagt ggcgcgctct ggctccagtc aatatcactt gcctgccact gatttaccgg
    23221 ttttttaccc gcgccgagaa tggctttcat cgaaggaatg cgtggcacgt taatatcgga
    23281 ggtgacgcag agcacggctg gaacagagag ttcaataact tcaacatcat cttcaagcgt
    23341 gcgttcaatc accagtgtat tgccctgacg ctgaatagca ctcactgcat taatcaccgg
    23401 aagttgcaga atttctccga ccagcaagcc aacctgctgg gcataaaggt cgccggaacc
    23461 ttcaccaaag atcagtaaat cgaagccgat cttttcaatt gctgccgcca gcgcttttgc
    23521 ggtatcgaga ggcagtgcat gttcaagttg cgcatcctgc accaaataca ggctgtgcgg
    23581 cccgcgggat agcacgtctt tgcgcacttt cgagttctgc aacaatgagc cgccaacggt
    23641 cagcgcggct atctcatcgt catctgttgc gagctggctt gcagcttcaa tggcattgag
    23701 atcgaactgg ctgattttgg cgtcggcatt gtcgaaattc agggtgtatt ctggagtgac
    23761 aacaatgtcc tgttcttcag gcaccagctt aaagcaggtt attattttca tgatttctcc
    23821 tgtgagtcgc atacggaacc atctccgatg gaaggtaaaa atggcctgaa aatagtgtgc
    23881 gatagcggtg cggggggttc gacggagcaa gctgttttcc ttgccaacaa tcttgcaaaa
    23941 aaaatgcgct tgctaaaaca aaagtgcaat ttgcggcaat atagttattc catgaccgta
    24001 caatcttgca ttcagtgtaa ataatcttgt ttagctctcg ctaaacgcgg ttgtataacc
    24061 gcaatacacg ccgtatttta tccggcatat attgtttttg cttgctcatg gaatagggag
    24121 gttgtcatgt atcaacgctg ttttgataat gccagtgaaa cgctgtttgt cgccggtaaa
    24181 acgccacggc tttcacgttt tgcatttagc gatgatccaa aatgggagtc tggacatcac
    24241 gttcatgaca atgaaaccga gctgatttac gtcaagaaag gggttgcaag gtttaccatc
    24301 gattcttcgt tatatgtcgc acatgcggat gacattgtgg tgatagaacg cggcaggctg
    24361 catgcggtgg cctctgacgt taacgatccg gcaacgacgt gtacctgtgc gctgtacggc
    24421 tttcagtttc agggggctga ggaaaatcag ttactgcaac cgcattcttg tccggtaatt
    24481 gccgcggggc agggaaaaga agtcataaaa accttattta atgagctaag tgtgattttg
    24541 ccgcaaagta aaaatagcca aacatcttcg ttatgggatg catttgccta
//
LOCUS       DAJTQY010000058        24173 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.058, whole genome shotgun sequence.
ACCESSION   DAJTQY010000058 DAJTQY010000000
VERSION     DAJTQY010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24173
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.058"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(15..587)
                     /locus_tag="OO349_002431"
     CDS             complement(15..587)
                     /locus_tag="OO349_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HCR5867466.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            805..1665
                     /gene="tesB"
                     /locus_tag="OO349_002432"
     CDS             805..1665
                     /gene="tesB"
                     /locus_tag="OO349_002432"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HCR5867467.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHCIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            complement(1714..3000)
                     /gene="amtB"
                     /locus_tag="OO349_002433"
     CDS             complement(1714..3000)
                     /gene="amtB"
                     /locus_tag="OO349_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HCR5867468.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSVGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(3030..3368)
                     /gene="glnK"
                     /locus_tag="OO349_002434"
     CDS             complement(3030..3368)
                     /gene="glnK"
                     /locus_tag="OO349_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HCR5867469.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            complement(3549..5330)
                     /locus_tag="OO349_002435"
     CDS             complement(3549..5330)
                     /locus_tag="OO349_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCR5867470.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGAVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIHLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDCIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIIDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            complement(5323..7095)
                     /locus_tag="OO349_002436"
     CDS             complement(5323..7095)
                     /locus_tag="OO349_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCR5867471.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLSLMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDVPEIREEAVDA"
     gene            complement(7125..7583)
                     /gene="decR"
                     /locus_tag="OO349_002437"
     CDS             complement(7125..7583)
                     /gene="decR"
                     /locus_tag="OO349_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HCR5867472.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGVWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            complement(7737..8555)
                     /gene="cof"
                     /locus_tag="OO349_002438"
     CDS             complement(7737..8555)
                     /gene="cof"
                     /locus_tag="OO349_002438"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HCR5867473.1"
                     /translation="MARLVAFDMDGTLLMPDHHLGEKTLSTLARLRECDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKESPALLQVFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVRTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            8654..10346
                     /locus_tag="OO349_002439"
                     /pseudo
     CDS             8654..10346
                     /locus_tag="OO349_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
     gene            10411..11106
                     /gene="queC"
                     /locus_tag="OO349_002440"
     CDS             10411..11106
                     /gene="queC"
                     /locus_tag="OO349_002440"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HCR5867474.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(11158..11556)
                     /gene="fadM"
                     /locus_tag="OO349_002441"
     CDS             complement(11158..11556)
                     /gene="fadM"
                     /locus_tag="OO349_002441"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HCR5867475.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGVENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILNQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(11650..12021)
                     /locus_tag="OO349_002442"
     CDS             complement(11650..12021)
                     /locus_tag="OO349_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HCR5867476.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            complement(12172..14043)
                     /gene="ppiD"
                     /locus_tag="OO349_002443"
     CDS             complement(12172..14043)
                     /gene="ppiD"
                     /locus_tag="OO349_002443"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR5867477.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLVAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGASG
                     INSDIITVDGDRAFVLRVSEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(14235..14507)
                     /gene="hupB"
                     /locus_tag="OO349_002444"
     CDS             complement(14235..14507)
                     /gene="hupB"
                     /locus_tag="OO349_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HCR5867478.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(14716..17070)
                     /gene="lon"
                     /locus_tag="OO349_002445"
     CDS             complement(14716..17070)
                     /gene="lon"
                     /locus_tag="OO349_002445"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HCR5867479.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDVPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVIGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(17258..18532)
                     /gene="clpX"
                     /locus_tag="OO349_002446"
     CDS             complement(17258..18532)
                     /gene="clpX"
                     /locus_tag="OO349_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HCR5867480.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(18658..19281)
                     /gene="clpP"
                     /locus_tag="OO349_002447"
     CDS             complement(18658..19281)
                     /gene="clpP"
                     /locus_tag="OO349_002447"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HCR5867481.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(19528..20826)
                     /gene="tig"
                     /locus_tag="OO349_002448"
     CDS             complement(19528..20826)
                     /gene="tig"
                     /locus_tag="OO349_002448"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HCR5867482.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(21170..21487)
                     /gene="bolA"
                     /locus_tag="OO349_002449"
     CDS             complement(21170..21487)
                     /gene="bolA"
                     /locus_tag="OO349_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HCR5867483.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            21792..22370
                     /locus_tag="OO349_002450"
     CDS             21792..22370
                     /locus_tag="OO349_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5867484.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRALRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            22414..23889
                     /gene="ampG"
                     /locus_tag="OO349_002451"
     CDS             22414..23889
                     /gene="ampG"
                     /locus_tag="OO349_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HCR5867485.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIITTLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVMLGGLLDYLALRKTHLT"
BASE COUNT         5665 a         6664 c         5821 g         6023 t
ORIGIN      
        1 agggttaacg tcgcttaata ttgcgtagga gcgggaactg ccgaagacgg attcacctga
       61 gttggtgaag tcgaaggtac ggtagtcgtt gcgccaccgc tggcctgaac cggcacggcg
      121 gtttgctgta ccggcaccaa tgtcaggtcg gctttagttc cgccctggtt gatcaccggc
      181 tgaacggtat cggtgataaa taccagtttg tcattcacgg taatcgccgc actcaacaga
      241 atacgcgcgt tcggctgaac atctgccggg ttaaatggca gaacaaagct gaatggtgac
      301 tgtttacctt cagtacgcac cgctttctgc gccagcactt ttgacggtgc atcggctaac
      361 gacgcgtcag aaagtgtcac ggtcagcaca gcatcaggcg gcagtgcgac tttctgacgg
      421 atccagacgg taccggagac attcggttgc tggatagcag gttgttgtgt tgctgaaata
      481 gacgtatttg cagccggggc tggcgtctga atatccgcgc ttttatctgc gcaagccgcc
      541 aacgcaatcg caaccgctaa accactggcc atgtgcacga gtttcattca tttctcctta
      601 ttatcaatgc accagcgggc taactttcct cgccggaaga gtggttaaca aaatagtaac
      661 gtcaacaagt gtggcacaca tcacgcattt ctgcctgtaa ttagcccgta attcagacta
      721 tttcacccat cggaccagtg gcaaatttgc gttatactca actcactttg gcttgctgcg
      781 gcagctttgt tactggagag ttatatgagt caggcgctaa aaaatttact gacattgtta
      841 aatctggaaa aaattgagga aggactcttt cgcggccaga gtgaagattt aggtttacgc
      901 caggtgtttg gcggccaggt cgtgggtcag gccttgtatg ctgcaaaaga gaccgtcccg
      961 gaagagcggc tggtacattc gtttcacagc tactttcttc gcccaggcga tagtaagaaa
     1021 ccgattattt atgatgtcga aacgctgcgt gacggtaaca gcttcagcgc ccgccgggtt
     1081 gctgctattc aaaacggcaa accgattttt tatatgacgg cctctttcca ggcaccagaa
     1141 gcgggtttcg aacatcaaaa aacaatgccg tccgcgccag cgcctgatgg cctgccttcg
     1201 gaaacgcaaa tcgcccaatc gctggcgcac ctgctgccgc cagtgcttaa agataaattc
     1261 atctgcgatc gtccgctgga agtccgtccg gtggagtttc ataacccact gaaaggtcac
     1321 gtcgcagaac cacatcgtca ggtgtggatc cgcgcaaatg gtagcgtgcc ggatgacctg
     1381 cgcgttcatc agtatctgct cggttacgct tctgatctta acttcctgcc ggtagctcta
     1441 cagccgcact gcatcggttt tctcgaaccg gggattcaga ttgccaccat tgaccattcc
     1501 atgtggttcc atcgcccgtt taatttgaat gaatggctgc tgtatagcgt ggagagcacc
     1561 tcggcgtcca gcgcacgcgg ctttgtgcgc ggtgagtttt atacccaaga cggcgtgctg
     1621 gttgcctcga ccgttcagga aggggtaatg cgtaatcaca attaaaaaaa tagccggagg
     1681 tgaaaaccgt ccggctattt tttgcagtgc ttgttacgcg ttataggcat tctcgccgtg
     1741 gctgttgacg tccagccctt ctcgctcttg ttcttccggc acacgtaggc caaccgtcag
     1801 atccgccagt ttgtagccaa taaatgccac cacaccggac cagacgatcg taatggcgat
     1861 gctttccaac tgtaccagca actgatggcc catcgtcaca ccttcagcga agcccacgcc
     1921 gcccagagaa ctggcggcaa aaatcccggt catgatacag ccgacaatgc cacaaacgcc
     1981 gtgcacaccg aagacatcgc agggatcatc cacccgcagc aagcgtttga gcatggtaac
     2041 gccccacaaa cccgccagac cagctaccac gccgataatc aacgcgccgc caaccccaat
     2101 gtagccgcac gctggcgtca cgccgaccag accggcaatc gcgccagaac acgctcccag
     2161 cagagaaggc ttaccacgca gcgcccattc accgaagatc cagccaagaa ttgccgccgc
     2221 cgttgcgacc acagtattca caaatgccag tgccgcgatt tcattcgccg tgcccactga
     2281 cccggcgtta aagccaaacc aaccgatata gagaatggca gtcccggtga agaccatcgg
     2341 caggttgtgc ggtttaaacg cctctttacc gaagcctacg cgtttgccta tcagatacgc
     2401 gcccaccaga ccggcgattg ctgcgttaat gtgcaccacc gtgccacccg cgaaatccag
     2461 cgcaccgtga gaagccagca aaccaccgcc ccacaccata tgcgcaatcg gaatgtaaga
     2521 gagcgtcagc cataccacca cgaaaatcaa cacagctgag aagcggattc gttccgccag
     2581 cgccccaact atcaagccta cggtaatgca ggcaaacgat ccctgaaacg ctacgtggat
     2641 atactgataa atgctgccca tcaccgccgt cagttcgatg ttttttagca tcaaccagtt
     2701 aatgttgccg aagaagttgt tgccctcacc aaacgccagc gaataaccgt aaaccaccca
     2761 gagaatacag accagtgcaa acgtcaccgt cacctgcgtc agcatcgaca gcacgttttt
     2821 gccgcgaatc aacccaccgt aaaacagggc aatccccgga atagtcataa acagcaccag
     2881 cgcagtacaa atcatcataa acgcattgtc ggctttatcg gccaccgcag gtgcagccat
     2941 taccagtccc ggcagcatcg ccagtgaagc aagcccagtt tttatcgtcg ctatcttcat
     3001 ttttcgttcc tgttgctgtg tgccagagat tacagcgccg cttcgtcggc ttcgccggta
     3061 cgaatacgaa tgacgcgttg caattcagcg acgaagattt tgccgtcgcc aatttttccg
     3121 gtgtaagccg ccttactgac gatatcgatc acttcatcga gttggtcatc agcaatcgcc
     3181 acatcaattt ttacttttgg caggaagttg acgctgtatt ccgccccccg gtacagctcg
     3241 gcatgccctt tctgacgccc gaaacctttc acttcggtga cggtcaggcc ctgaatacca
     3301 atggaagata acgcttcacg aacgtcttcc agcttgaatg gtttgattat cacggtcacc
     3361 agcttcatag atcccctccg gtcagaattc ggtaatggtt ctgctacacg aagaaggtat
     3421 tgcaagcggt gtgccagaaa tgaaaaacga gaaagagagc aggaagttaa gaagattgaa
     3481 aatgaaaacg cactatgaca gtgcacagtg cgttacattt ttgcaccaac gatgaaatat
     3541 tgcgctattc aggcgctcaa tgattcctct tcacgcacgc tggctgccag ctcttcgccc
     3601 gcaagttgca gttgatacat ctgccagtag cgtccctggg ccgccagcag ttgctggtga
     3661 gtgccctgct ccacggcttg cccacgatga agcaccagaa tggtgtcggc atcaataata
     3721 gttgataagc ggtgagcaat caccaccagc gtggtatgtt cacgcaccgc cgccagagca
     3781 tgttgaatcg cctgttcagt accggagtca atgctggcgg ttgcctcatc aaggatcagg
     3841 atttgcggcg tctccaccag cacgcgcgcc agtgccagca gttgcttttg cccgactgag
     3901 agattatttc cctgctcgcc cagcggcgta taaatacaat cactcatgct gcgcgccagc
     3961 tccgccagtt gcacggtttc cagcgcctgc cagacgcgtt cttcggagat atcccgcccc
     4021 agcgtcacat tagcgaggaa ggtatccgcc agcaccaccg gatcttgctg caccattgcc
     4081 acgccctggc gcagcgcgct atgacttagc gaacttaatg gacgaccatc aaggtgaatc
     4141 tcaccttccg ttagcgggta atagcccatc aataaactgg cgagggtact tttaccactg
     4201 ccggtatgcc cgaccagcgc cacaaagtta cgcgaaggca cagagagatt aatgttcttt
     4261 agcaccagat tgtcatcgcg ataagcaaat gacacgttat cgacttcgat ggtgccactc
     4321 tgtaacgggt aatcatcatt gccatattgc tggcgcggtc cgtccatcag ttcaaacacg
     4381 cgctcaccag caacaacagc ctgttgcagc atcgcctgtt gcgtggtcag ttcgattaag
     4441 ggttcgttaa gtcgcccaag atagctgata aacgcataca acacgcccac ttcaatggtg
     4501 ccgctggcgg agaagccaaa cagcatcaat aagccacaaa gaatgagcga tgaaaacaga
     4561 ctcagcagcg gacgcagcag aaaaccgtcg aggcgcaggg tttgcatcct agccatatag
     4621 tgtgaacgac tggcctcccc catacgttcg ccaaatcgcg cctgctgacg aaactgctgg
     4681 ataacgctca tgccattgat gatttcgtta aagccgtcgt tgatatccgc cagataggcg
     4741 cgcacacggc ggacaatcgg cgtgctgtaa cgctggtata tcaccattac caccagcacc
     4801 accgggaaaa tcattatcgc caccagcgcc attcgccagt cgaggctgaa catcgccacc
     4861 agcatcgcgc ccaccagtgc ggcactgcgc aggacagttg ccactacggt aacgtagaga
     4921 tcgcggatca cttcagtgtc attagtgacg cgggaaatca cctgcccgac gggttgggta
     4981 tcaaactcgc ttaatggctg gcgtaacgca gcatccatca cgtcggtacg caactgttgc
     5041 actgcgccta ctgccgcccg attaaacagc agcgactgcg cgtaatgtag cccggcggca
     5101 aacagttgta gcccaacata cgccgcagcc agccctgcaa ccactttcaa cggcaggtta
     5161 tttttcgcta ccatattgtc gataaaatag ctgataagca gcggcccact gacttctgcc
     5221 gccgccgcaa cccacatcat caggaccgca atccccagcg gtttacgcca cggcgaacca
     5281 tacgctaaca ggcgcttgag agtcggccac agttggctaa aactacgcat cgacggcctc
     5341 ctcgcgaatt tccggaacgt cgtcgagcgc cgcctccagt tgttgatagc gatacatatc
     5401 gcgataccaa ccgctttgtt gcgccagctc atcatgattg ccacgctggg cgatatgtcc
     5461 gtgctgcatc acaataattt cactggcttc cgtcagtgcc gaaaggcgat gggcactgat
     5521 gattaccgtt cttccctgtc cccactgacg caggttatgc aggatctggt gctctgtgcg
     5581 tccgtccacc gccgaaagcg catcatcaag gatgaggatt tccgcgttga ctaataacgc
     5641 tcgagcaatg gagatacgct gtttttgccc accggaaagc atcacgccgc gctcgcccac
     5701 ttctgtatcg taaccttgcg gtagacgcaa aatatcgtca tgtacgctgg ctaaccgcgc
     5761 gacatgctca atctcttgct gggtggcatt cgggcaaccc agcgcgatgt tattcgccac
     5821 ggtgtcagaa aaaaggaatg gcgtctggct gaccaccgcc aggcggctac gccagctatc
     5881 gagttgtaac ttcgtcagag gaatatcatg aaagcgaata tccccctcgc tgacgtcgaa
     5941 atgacgctga atgagcgaca acagggtact tttgccggaa ccagtcggcc cgcaaatacc
     6001 cagcatctga ccgggtttca gggcgaaatt gacgttttcc agcgcaggat ggtcagtctg
     6061 cggatacgtg aactggtgaa tatttacatc cagttcgcca cgcccttccg gcacgggttc
     6121 gctaccatcg ttcaccaccg gcgcttccgc cagcatcgcg cgaatacggc tgtatgcagc
     6181 actaccacgt tccacaatgt taaacatcca tgccagcgcc agcattggcc aaatcatcag
     6241 acctaaatac atcataaaac tggtgagctg gcccagcgtt aaactgccct gcaccaccat
     6301 ccaactaccg ccgccaatcg ccagcaagtt cgccatacca atcgcgatat agatggtcgg
     6361 gtcgaagcga gcatcaatac gcgccacccg catgtttttt ttgccggtat cttcggcatc
     6421 cgcggcaaat aacgccgact ggcgatcttc cagaccaaag gctttgatca tgcggatact
     6481 ggtgaggctt tcctgggtgc ggtcattaag actggaaaac gccgcctgtg ccagcttaaa
     6541 gcgttcatgc aaggcatcgc cgttgcgctt aatcatgatc gccatcactg gcatcggtaa
     6601 cagggcaaat aaggtcaact gccagctaat ttgcgtagac atcataatca acacagcgca
     6661 gcccattacc agtgaatcca ccagcgtcag caccccttct ccggcggcaa acacgacgcg
     6721 atcgacgtca ttggtcgcac gagccatgag atcacccgtg cgatgacgca ggtaaaactc
     6781 cgggtgctgc cggcttagct gacggtaata atcttcacgc agttcaacag ccagttggta
     6841 agacgcgcca aacagcaata cgcgccagac ataacgcagg agataaacca caacggcaat
     6901 cagcaccatg gtggcgatcc acatcaggct cagcccggta gtaaagtgtt gttctgtcac
     6961 gccatcgaca acaataccaa ccacttttgg cggaaccagt tgcagcatcg cgataatgac
     7021 aagcaaggcg acagccccaa gatagcgacg ccattcccga cggaaatacc agcttaattg
     7081 agcaaataat cgcacgcgtt gtgtcctgac ctgattctgg atatttattc gatgggtaaa
     7141 gaagtggtgt atttaatctg ttccatcgcg aagctggaag tgacgtccga cagccccggc
     7201 acgctgttta ccagacgctt ataaaactcg tcgtagcgtt tcatgtcggc aacctggacg
     7261 cgcatcagat aatcgtattc accagccatg cgccagaccc ccagcacttc tggcatttcg
     7321 gtaaccaccg tgacaaagcg gcaataccat tcgctgctgt gatgttgcgt ttttatcagc
     7381 acaaaggcgg tcaggccgag gcctattttt tccggatcca gcagggcgac tttgccgata
     7441 aggataccgt cgtcctccag ccgtttcagg cgcttccagc agggggtggt tgtcagatta
     7501 acggcttcag ccagtgcctg caaagagagg gtgcaatcct gctgcagtaa ggccagcagc
     7561 ttacggtcaa ttttatctaa catagccctt ccacggagaa tttttttctc ttttaattct
     7621 attttaaagg tgaaatcgcg acaatttatt ctgtgcggtt taaacaccac ggcacaaaat
     7681 gacaaattca acgcaaaaaa aaaccgggca attgcccggt gctggaaggg atctcgttat
     7741 tcgggggaat aaggtagatg tggatagtcc agccagtgcg tcaaatagtg agagacagcc
     7801 tgatttcggc aatgtccaat caccggtaaa tgcgggagct ccgcgcgcag ttgcggcatc
     7861 gcattgccca taataaaccc gctaccgacg ctgcctaaca tttcgcgatc gttcatcgca
     7921 tcaccaaagg ccatgcaatc gcgcaacgat aaacctaaat gttgggtccg caccgtcaat
     7981 gcagcgcctt tattgcagcc caccggcagc acttcgaggc aatccgtggc ggaaaaacac
     8041 aaatgtgcac gctcgcctaa tgcttcgtat agctggatct gcaagcgtgt aagatcgtcg
     8101 tgatcgccac agaagcagat cttggtgacg ctgccgagtg gcattttttt gacatcgatt
     8161 atctgataac gaaaaccgct atagacaaat acctgcaaca acgcagggct ctctttcccg
     8221 gtaaaccaac cgtcgtcatt gaagatatgc atgctggctc gggtatccca ttgctgatac
     8281 agcaccagct ccgcgacatc cgcaggtaaa tcatcacgat gtaaaagttc accttccaga
     8341 gaatgcacgc gcgttccgtt gccggtaatc aaatatgcat ccagcgatag cgccccgaga
     8401 atatgctgca tctccagcgc atgacgcccc gtggcaaaag tgagggtaat gtcgcattca
     8461 cgcagtcgcg ccaaagtaga gagggttttc tcacctaaat gatggtcagg catcaataaa
     8521 gtgccatcca tatcaaatgc taccagacga gccatttctt tctccactct gtgacacggt
     8581 taatggtgat tgagtatcac ctgatatata cggaagtaat agtgaatagt taataatatt
     8641 gttccgggtt tatatgcgat tgctcaaccg tcttaaccag tatcaacgtc tgtggcaacc
     8701 ttcagccgca aaccgtcacc gtcagcgaac tggccgaacg ctgtttttgc agcgaacgcc
     8761 atgttcgtac gctgttgcgt caggcacagg aggcgggatg gctggagtgg caggcgcagt
     8821 caggacgcgg aaagcgcgga caattacgct ttctggtcac gccggaatcg ctacgcaatg
     8881 cgatgatgga acaggcactg gaaaccggaa agcagcaaga tgtgctggag ctggcgcaac
     8941 tggccccagg tgagctgcgc actctgttac agccatttat gggcggacaa tggcaaaacg
     9001 atacacccac gttgcgtatt ccctactatc gcccgctcga accgctacaa ccaggctttt
     9061 tgcccggccg tgccgagcag catctcgccg ggcagatatt ttccggcctg acccgcttcg
     9121 ataataatac tcagcgcccg attggcgatt tagcgcatca ctgggaaacc tctactgaca
     9181 ggttacgctg ggacttttat cttcgttcaa ccctacactg gcataacggc gatgcagtaa
     9241 aagcctcaca cttacaccag cgattattga tgctgttaca actgccagca ctggatcaat
     9301 tatttattag cgtgaagcgt attgaagtca cccatccgca gtgtctgacc ttctttttac
     9361 atcgccccga ttactggctt gcgcaccggc tggcgagcta ttgcagccat ctggcgcatc
     9421 cgcaattccc actgatcggc actggtcctt ttcgcttaac acaattcaca gcggagctgg
     9481 tgcgcctgga gagccatgat tattaccatt tgcgtcatcc gctgcttaaa gcggttgagt
     9541 actggataac tccgccgctt ttcgaaaaag atttgggaac cagttgtcgg catcccgtgc
     9601 aaatcaccat cggcaaaccg gaggagctgc aacgggtcag ccaagtcagt agtggcatca
     9661 gtttaggttt ttgctattta acgttgcgca aaagtccccg actctccctc tggcaggcgc
     9721 gaaaagtgat ctccattatt catcaatccg gtttattaca aacgttagaa gtcggagaaa
     9781 acctgatcac cgccagtcat gcattactgc taggctggac tattccgcac tggcaagtac
     9841 cggatgaagt caaactaccg aaaaccttga cgctggttta tcacctaccg atagaacttc
     9901 ataccatggc agaacgccta caggcgacac tggcagcgga aggctgtgaa ctcacaatta
     9961 tttttcataa cgcaaaaaac tgggacgaca cgaccctact ggcacacgca gacctcatga
    10021 tgggcgacag attaattggc gaagcaccgg aatatactct ggagcaatgg ctacgttgcg
    10081 atccactatg gccacatgtt ttcgacgctc cagcatatgc acatctgcaa tcgacgctgg
    10141 acgcggtgca agtaatgcct gatgaggaaa accgatttaa tgccctgaaa gcggttttta
    10201 gccagttaat ggcagacgcg acgctgacgc cgctgttcaa ctatcactat cgcattagtg
    10261 cccctcccgg cgtgaacggt gtgcgactga caccgcgcgg ctggtttgaa tttaccgaag
    10321 cctggcttcc cgcgccgtcg caatgaatag ctggtccggg ttaatcgcag gcgttaccat
    10381 aacgctttta ttgttcaagc aggattatct atgaaacgtg ctgtcgttgt gttcagtgga
    10441 ggccaggatt ccaccacctg tctggtgcag gcattacaac aatatgatga agtccattgc
    10501 gtgacgttcg attacggtca gcgacatcgc gcagaaatcg acgtggcacg cgaactggcg
    10561 ctgaaactgg gagcacgcgc gcataaggtt cttgatgtaa cgctgctcaa cgagctggcg
    10621 gtcagcagcc tgacacgtga cagcattccg gtgcctgatt atgaacctga agccgatggc
    10681 atcccgaata cgtttgtccc agggcgtaat attttgttcc tgacgctggc ggcaatatat
    10741 gcgtatcagg taaaagcaga agccgtaatt accggcgtct gtgaaacgga tttctccggc
    10801 tacccggatt gccgcgatga gtttgtgaaa gcactaaacc atgccgtcag tttgggcatg
    10861 gcgaaagata ttcgttttga aacgccgctg atgtggattg ataaagcgga aacctgggcg
    10921 ctggcagatt attacggcaa actggattta gtccgtaacg aaacgttgac ctgctataac
    10981 ggcattaaag gcgacggttg cggtcattgt gcggcatgta atttacgcgc caacggtttg
    11041 aatcattatc tggccgataa accgacggtg atggcagcga tgaagcagaa aaccgggttg
    11101 aggtaattat tccgggtgtc gccggatgcg gcttgagcat ccggcaccac aaaacgttta
    11161 cttaaccatc tgctccagct tttcgcgcaa ttccccttcc agagctaatg ctttctgcgt
    11221 tttaagatca atacaaacaa acgtaataag cgcatccgct accacctgcc cttccggctc
    11281 cagtgtaatg acctggttta agatgccgct tttaccgttt aattgctgca actgactggt
    11341 aatagttaac aggtcgctta ataccgctgg gcgacgatag ttaatattga tattgaccac
    11401 gacgaaggcg atgttatggg ccgtcatcca ctgaaaactg tcgctatttt ccaccccatc
    11461 ccagcgggct tcttcgagaa attcaaggta gcgggcgttg ttgacgtgct ggtaaacgtc
    11521 gagatgatat ccacgaactt tgatttgtgt ttgcatagcg caataacctt acgttgttgt
    11581 tataagtaca gaggtcataa ctggtatgac ctcttcagtc tggcaaaaaa ttgccactat
    11641 gcaaattaat tacagggtta ataccgccag attacgttcc accagcgaat tgcccatccc
    11701 cggcacctgc tttagatcct ccaccgtttt aaacggaccg tactcttcgc gataactgac
    11761 aatcgcctgc gctttcttca ggccaacgcc attcatcgcg cgggctagct cttccgcgct
    11821 ggcattatta atgctgaccc gggtgccttc ttcgtcactg gctttcgccg gtactgctgc
    11881 tttactttgt gctgcaggag cttccgcttt ggtttctacc gccgtcggtt tcgccacaga
    11941 agctgccgcc agcgcgctat gagacattcc ggcacaggcc agggacaggg taatgagcag
    12001 tgctttaatt ccgtgtttca tactgttttc tccttgtttg ttaacagtgg aatcacctta
    12061 acgggagtga aaacagtaaa caaatggcaa tgttcagaaa tggaaaaggc cgcgaaagcg
    12121 accttttctt gttacagctg caacgcattt ttttgcgagg cggcttcagg attattgctg
    12181 ttccagcgca tcgccaattt tgattttcgc ctctttacgc aggttactca tcagagcttc
    12241 aaagacgatt tgtgcgttgt tctgggtgat accctgcacc atcgcttttt tctgatcttc
    12301 cggcattgaa ccttgtttca cttcatccag cgccagcaga accacattac cttgcatatc
    12361 ggtcgccata ccgtagctcg gtttgtcttt cgctggcagt ggcagtgcaa acgccgcctg
    12421 gctaatcggg tcacgaccgg aacggcttaa ggttttcggc tcgccaaatt tcagaccggc
    12481 agcctgcata gcttccgcac ctttgccggc tttcaaatca accagcagtt tctcagcatc
    12541 cactttcgcc tgttgttcag ctttgttgtg ctgaaccagt gccttaactt gttcctgaac
    12601 atctgccaac ggtttcaccg cttccggttt gtgctcgctg acgcgcagca cgaatgcgcg
    12661 gtcgccgtct acggtgatga tgtcagagtt gatgcccgac gcgccgtttt cacctaccag
    12721 accgccgtta aagatagcgt cggcaaccgg cttgaagttc aactcttccg gcaggttatc
    12781 tttgctgaac caacccgtct gagtggcttt aacgccggca gcttgctctg caccggccag
    12841 agactcggtg tcgttgcttg ccgcatcgct cactttctgc tgcagcgcat agtacgcatc
    12901 gagggctttt tcgtgtttca ctttcgccgc aatgtcgtca cgtacttcgt ctaacgattt
    12961 cactttcgct ggctgaatgt cgtccagacg tacaatcagg aaaccgaccg aagatttgat
    13021 gacaccagac agttggcctt tttctttcag accagcattt ttcagttcgt cggggatagt
    13081 ggcatcttct aaccaaccca tatcgccgcc gttacgggca gagataatat cggcagattt
    13141 ttctttggct aatgcagcaa aatcaccgcc tttattcagc tcatcaagta ccgctttcgc
    13201 ttcatcttca gttttggtct ggatgatgct gtagcgggta cgctgcggct gggtgaattg
    13261 atcctgatgc tgatcgtagt agctctggat atccgcatcg ctaaccggtt gctgcatcgt
    13321 tgcggcaacc agcttgatgt aactcacgcg gaattgttcc ggcgtcatga aattgttttt
    13381 gttttgttcg tagtagctgg caatctcctg ttcggtcaca ggctgcttcg ccgccagcgc
    13441 gttaacatcg atagtcgcct cacgcaccac gcgttgttgc gcgaccagtg ccgccagctc
    13501 gtcggtttca cctttcagca taaaatcggt accggcaacg ccgttaatca gctgttgggt
    13561 agtgagctgg ttacgcagcg cctgggcgta ctgatcggcg gtcatcccca tctggttgag
    13621 gataccgtta tagcggctgt tatcaaattt gccgtcaacc tggaaggctg gggtcgcgaa
    13681 aatcgcctgt ttaacctgct catcactgat acccagtttc agctcacgag cgtactgatc
    13741 caacagcgcc tcgtcgatca gacgattcag cacctgttga cgcagggttt tcatatagcc
    13801 ttcgttcgct gccagctcgg agtattgatc gcccagctgt tgctgcatac gattacgctc
    13861 gctgttaaag gcgttctcga actgcccacg gctgatttcc tggtcattca cttttgcggc
    13921 gtagttattg cctccgccaa tcaggtaacc actcacgccg gtcaatatga acgacacgat
    13981 aatgataccg aaaataatct tgagcacgag actgtttgca gccgtgcgta agctgtccat
    14041 catggtgtaa caacactccg ctgtagatga ctgtgtacct cacgcaacgt atcgatgcgc
    14101 gcaaaggcct attgtgacaa gaaacggggg caattgtcag cccacaagac acagaaagtc
    14161 accgaataca aataaaaaag gcacatcagc agatgcgccc ttgaacttcg tcacatcccc
    14221 actggggaca acgcttagtt taccgcgtct ttcagtgctt tacctgcacg gaagctcggt
    14281 actttagcgg cagcgatggt gatctcttta ccggtctgcg ggttgcggcc agtacgggca
    14341 gcacgctctt taacggcaaa agtaccaaaa cctaccagtg ctacatcatc cccttctttc
    14401 agagattcag ttacggaagc aataatagca tctaacgcac ggccagccgc agctttagag
    14461 atatcagccc ctgcagcaat cttgtcgatc aattgagatt tattcactct tctcttcctc
    14521 tttataattt atatcgcact tgaatccttc aaggtacgaa cgcgcagcag ttatatcagg
    14581 ccagccatcc ccttacaaca ccccttaata agggcaagcc cgatccgcta tctaacttag
    14641 cgagacaaaa aaaggctggc aagcccgaat tagcctgcca gccctgtttt tattagtgca
    14701 ttttgcgcga ggtcactatt ttgcagtcac aacctgcatg ccagacggtt cattttgcag
    14761 cgccagagtc agaacttcct caatgcgctt cacaggatga atgtccagat cggcaattac
    14821 gttgtcagga atctcttcca gatcgcgttt attttcgaac ggaattagca ctgttttaat
    14881 cccgccgcga tgcgctgcca ggagtttttc tttcaaacca ccgatcggca gtacctgacc
    14941 acgcagagtg atctcaccgg tcattgccac atcggcacga accgggttac cggtcaggca
    15001 agaaaccagc gcggtgcaca tagcaatacc ggcactcgga ccatctttcg gcgtcgcacc
    15061 ttccggtacg tggacgtgga tgtcacgttt ttcgtaaaaa tcagggttga tccccagttt
    15121 ttccgcacgc gcacgaacca ccgttaacgc cgcctgaata gactcctgca tcacttcgcc
    15181 gagcgaaccg gtataggtca gtttgccttt acccggaaca catgcggttt caatggtcag
    15241 caagtcaccg cccacttccg tccacgccag accgattacc tgaccgacac ggttttcgtt
    15301 atcagcgcga ccatagtcga aacgctgaac accgaggtag tcatgcaggt tatcgccgtt
    15361 aatttcgata tgttttaatg acttatcgag cagtaactgc ttaaccgctt tgcggcacag
    15421 tttggagatt tcacgctcca gaccacgcac gcccgcctca cgggtgtagt aacgaataat
    15481 gccgataatg gcgctatcgt cgacggtcag ctcacctttt ttcagtgcat tacgttcaat
    15541 ctgcttcggc agcaggtgac gtttggcgat gttcagtttt tcatcttcgg tataaccgga
    15601 gaggcgaatc acttccatac gatccagcag cggtgccgga atgttcatgg agttcgacgt
    15661 cgcgacaaac atcacgtcgc tgagatcgta atccacttcc aggtagtggt cgctgaacgc
    15721 tacgttctgc tctggatcca gcacttcaag cagtgcagag gccggatcgc ctcgcatgtc
    15781 agaagacatt ttgtcgatct catcgagcag gaacagcgga tttttcacgc ccactttcgc
    15841 cattttctgg atcagtttac ccggcataga accgatgtaa gtacggcggt gaccacggat
    15901 ttccgcttca tcacgcacgc cgcccagcgc catacggaca tatttacgcc cggtggcttt
    15961 ggcaatggac tgaccaagag aggttttacc tacccccggc ggccctacca ggcagaggat
    16021 cggtcccttg attttgttga cacggctttg aaccgcaagg tactcaagga tgcgatcttt
    16081 cacgcgctcc agaccataat ggtcggtatc aaggatttcc tgcgcctgac gcaggtcttt
    16141 tttgaccttg ctgcgcgcat tccacggtac ctgtaccatc cagtcgatat aaccacgcac
    16201 tacggtcgct tctgccgaca tcggagacat cattttcagc ttctgcaact ctgcttccgc
    16261 tttctctttt gcctctttcg gcatcttcgc cgcgtcgatt ttgcgcttca gggcttcgtt
    16321 ttcgtccggc acgtcgtcca tttcaccgag ttctttctga atagctttca tttgctcgtt
    16381 cagatagtac tcacgctggg atttctccat ctgcttttta acgcggttgc gaatgcgttt
    16441 ctcaacctgc agcagatcga tttccgattc catcattgcc atcagatatt ccagacgttc
    16501 gttaacgtcg gacatctcca gaacggactg tttgtcagcc agtttcagcg gcatatgtgc
    16561 agcaatggta tccgccagac gcgccggatc gtcgatgcta ttcagcgacg tcagcacttc
    16621 tggtgggatt tttttgttca gcttgatgta gccttcgaac tggctgattg cagtacgcac
    16681 cagcacttcc tgttcccgct catcaatggt cggcgactcc agatactccg ccttcgcaga
    16741 aaagtgttcg ccattgtcag agagcgcaga aatacgcgcg cgctgtaacc cctcgaccag
    16801 cactttgacg gtgccgtcag gcagtttcag catctgcaat atagaggcca cggtcccgac
    16861 ggtgaaaaga tcgtttacac ccggctcatc cgttgaagct tctttctgcg cgaccagcat
    16921 aattttttta tcatggtcca tcgccgcttc cagacaacgg atagattttt cccgcccgac
    16981 aaataagggg atgaccatgt gcggataaac caccacatcg cgcagcggca atacggggat
    17041 ttcaatgcgt tcagaacgct caggattcat agagctctct cttagtttaa tttccgccag
    17101 gtaatcagat gacacgactg tgcttcacgc catctattaa catgtacgtc agtatatggg
    17161 gatgtttccc ccacattcaa cgccgagaat agaggaaaaa ttaaagggga gataaaatcc
    17221 cccctttttg gttaactaat tgtatgagaa tggttaatta ttcaccagat gcctgttgcg
    17281 cttccggctt accataaatc agcaacggtt tgctttgacc atcaattacc gactcgtcga
    17341 taaccacttt ttcgacgtct tccatggacg gcagatcgta catggtatcg agcagtgcgg
    17401 cttctacgat ggaacgcagg ccacgggcac cggttttacg cgccatcgct ttcttagcga
    17461 tagcatccag cgcctcgtca cggaattcca gatccacgcc ttccagatta aacagcgcct
    17521 gatactgctt ggtcagggcg tttttcggct ctttgaggat ctgaatcaga gcttcttcgc
    17581 tcagttcatt caacgttgct acaaccggca gacgaccaat gaactcaggg ataagaccaa
    17641 acttgatcag atcttccggt tcaacctgcg ccagcagctc gccttcgctt gctttgtcgg
    17701 acttcgcttt taccgtcgcg ccaaaaccaa tgccggagcc ggtttctaca cggtgggaaa
    17761 tcactttatc cagaccggca aacgcaccgc cacaaataaa caggatctta gaggtatcaa
    17821 cctgcaagaa ttcctgctgc ggatgtttac gcccaccttg cggtggaaca gcagctaccg
    17881 taccttcgat cagtttcaac agtgcctgct gtactccttc accggaaacg tctcgggtaa
    17941 tggacgggtt gtctgactta cgagaaatct tgtcgatttc atcgatgtag acaatcccgc
    18001 gctgcgcttt ctgtacgtcg taatcgcact tttgcaacag cttctgaatg atgttttcaa
    18061 cgtcttcgcc cacataaccg gcttcggtca gcgtggttgc gtcggccatg gtgaacggga
    18121 cgtccagcag gcgcgccagc gtttcagcca gcagcgtttt accggaaccg gtcggaccga
    18181 tcagcagaat gttacttttg cccaactcga caccattgct ggtatcgccg ttgcgcagac
    18241 gtttgtaatg gttgtatacc gcgaccgcca gcactttttt cgcctgttcc tggccgataa
    18301 cgtaatcgtc caggtggttg cgaatttcat gcggcgtcgg tagcgcactg cgttcacgat
    18361 gcggtgcaac ttctttaatc tcttcgcgaa tgatgtcgtt acataaatca acacattcgt
    18421 cgcagatata cacggatgga ccggcaatca gcttgcgcac ttcatgctgg cttttgccgc
    18481 aaaaagagca atacagcaat ttgcctgagc catctttgcg tttatctgtc atgggtcaaa
    18541 acctcttctt tgttctttgt gccgcacacg acgacgcaaa tgccattctc aggcgcaagc
    18601 cgcttacagc gttgtgccgc cctagataag tatagcggca cagttgcgcc tctggcatca
    18661 attacgatgg gtcagaatcg aatcgaccag accgtattcc accgcttcag gggcggaaag
    18721 gaagcgatcg cgctcggtat cacgttcaat ctgttctaat gattgacccg tatgaagcgc
    18781 cataagttca ttcatgcgcc ctttaacttt cagaatttca cgggcatgaa tttcgatatc
    18841 ggtcgcctgg ccctggtagc cgcccaacgg ttggtgaatc atcacgcgcg aattcggcag
    18901 gcaaaaacgt ttaccttttg cccctgcggt caacaagaaa gcgcccatcg aggccgcctg
    18961 gcccatacag atggtgctga catcaggctt gataaactgc atggtgtcat agatagacat
    19021 cccggcagtg atcaccccgc ctggggagtt aatgtacaga tagatatctt tttctgggtt
    19081 ttccgcttcc aggaacagca tctgcgccac aatcaggtta gccatgtggt cttcaacctg
    19141 gccagtcaga aaaatgacgc gttccttaag tagacgagaa tagatatcaa aagagcgctc
    19201 accgcgtgag gtctgttcaa tgaccatcgg caccagcgcc atatggggtg caaagttatc
    19261 tcgttcgccg ctgtatgaca tttccgtctc ctggataaaa ttgaaaaaaa cctgctgtac
    19321 cgattgtaac cttatggacg gattatcagc tagtccctgt gttatgggta cggcatcacc
    19381 atatttcaag cataacaatc ttttgttgtt acgctaacac cgaaagaggc ttttcgcacg
    19441 gctccatgca aaattcatga caaaaaaaag cccaccacct ggcggtgacg ggcctttgtg
    19501 cgaatttagc gcgttatgct gcgtaaatta cgcctgctgg ttcatcagct cgttgaaagt
    19561 ggtttctttt tcagtcactt tcgctttcgc cagtacagct tcaacagcct gttcttccag
    19621 agcaacattg cgcatgttgt ccatcagttc tttgtttttg ctgtagaact cgataacttc
    19681 tttcggatct tcgtacgcag aagccatctc ttcgatcagg cctttcacgc gctcttcgtc
    19741 agctttcagc tcgttggtgc ggataacttc gcccagcagc aggccaacaa ctacgcggcg
    19801 tttagcctgt tcttcgaaca gttcgcgcgg cagttccaga gcttgttttt cgttgccacc
    19861 gaaacgctgt gcagcctggc gacgcagaac gtcgatttcg ctgtcgatca gcgcagccgg
    19921 tacgtcgatg tcgttagctt ttaccagacc ttcgatcgcc tgagacttaa cgcggttacg
    19981 gatggcgctc ttcagctcgc gctccatgtt tttacgcact tcagcgcgca gaccttctac
    20041 ggaaccatct tcaacgccga aacgtttgat gaattctgca gtcagttccg gcagttcacg
    20101 ctcttcaact ttcttcaggt tgatagcgaa tttcgctgct ttacctttca ggttttctgc
    20161 gtggtattct tccgggaagg tcacgtcgat ggtgaactct tcgccagctt tgtggccttt
    20221 gataccgtct tcaaagcccg ggatcatacg accctggccc atcgccagta cgaaatcaga
    20281 cgctttaccg ccttcgaact cttcgccgtc tacagaaccg gtgaagtcga tggttacgcg
    20341 gtcttctgct tcaacagcgc cgtctttttc tttccaggtc gcctgctgtt tacgcagagt
    20401 atccagcatg ccgtcaacgt cagcgtcggt cacttcaacg atcggttttt caacttcgat
    20461 cgcttccaga ccctgcagtt caacttccgg ataaacttca aactctacag agtaagtgaa
    20521 gtcttcaccc agcttgtatt cgcccggaac ataagtcggt gcgccagccg gattgatttt
    20581 ttctttaatg atggcgtcaa tgaagttacg gctcatcagg tcacccagaa cgtcctggcg
    20641 tacagacgcg ccataacgct gagcaacgat attcattggc actttgcctt tgcggaagcc
    20701 gtcaatacgt acttttttcg caacgttgac cagctcgctt ttaacagcgg tctcgatgct
    20761 gtcagcagcg atagtaatcg ttacacggcg gccaaggcct tgagtggttt caactgaaac
    20821 ttgcatcttg ttacctcaaa aaatcacagt gctcggtcaa ctcttacccc gcaagcacag
    20881 gttgttggct tcgcggaacc gggatgttct tataatcaat cacattccct gttaccagaa
    20941 tcatcccgaa agcattcaaa taggacgcgg cattatagcg gcatcacttt tatgagtcga
    21001 gaacggttat tgcgcgctgt cgcgtatttt ttcacaattc ccgggcaatt ttcgtctgaa
    21061 tgctgggcaa aaagcgcaaa atccagacaa aacaaaacgg cccgcaggcc gtttttcata
    21121 aaatctttag caacatactg gaaaagcgcc gacagttgca aatgcgtttt tacgcgatgc
    21181 ttcctgctcc acgacaggga ggagaggcaa agacggtgtc ctgcaaccct tcccactcct
    21241 taatagtgta agtatgcaga gccagcgcat gaacggtagt agagagttcc tccgctaaag
    21301 tactgtaaat cattcgatga cgattcagaa aacgttcacc cgtaaaacga tcgctgacca
    21361 gcacaacttt aaaatggctt tcagagccgg ctgggacatt gtgacgatag ctttcatcca
    21421 ctacttcgag gaatacgggt tggaacgccg cccttaattt ttcttctatc cgctcacgta
    21481 tcatcatgaa atatctcctc cgacaacgct gagatgtcat ccatcccttt aaatactagc
    21541 cgcttttacc gtttccattg cagcttaaca acaaatattt aggtttcgtc ctggtttttc
    21601 cgtcaagcgt atgaagttta ctgatttaaa gattgaaaca caggcctcgc atcagcgttt
    21661 cttttaccag gggtgattat ttcgtcaaca tgatgaattt acatgcctta cccacttccc
    21721 ctcgccgttg gcaatgttat gatggcggga atttttacca aacacgctta agagtctctg
    21781 agagcccgaa catgttcaaa aaaatcctct tcccgttagt tgctctgttt atgcttgcag
    21841 gatgcgcaaa accgccaaca actattgaag tttccccgac gattacactg ccacagcagg
    21901 atccaagcct gatgggcgtc accgtaagca ttaatggtgc cgatcagcgt accgatcagg
    21961 cgctggcaaa agtcacccgc gataatcaaa tcgttaccct gaccgcctcc cgcgctctgc
    22021 gtttcctgct gcaagaagtg ctggaaaaac agatgaccgc gcgtggttac atggttggtc
    22081 cgaatggccc ggttaatctg caaatcatcg ttagccaact gtatgctgac gtgtcccagg
    22141 gcaacgtgcg ctacaacatc gctaccaaag cggatatcgc catcatcgct accgcgcaga
    22201 atggcaacaa aatgaccaaa aactatcgtg ccagctacaa cgttgaaggt gcgttccagg
    22261 cctccaacaa aaatatcgcc gatgcggtta acagtgtgct gaccgatacc atcgctgata
    22321 tgtctcagga caccagcatt cacgaattca tcaagcagaa cgcgcgttaa tttctgccct
    22381 ctggcccggt gcaagccggg cctgtagacg cccatgtcca gtcaatattt acgtattttt
    22441 caacagccgc gttcagccat attgctgatc ctgggttttg cttccgggct accgctcgcc
    22501 ctgacatccg gcaccttaca ggcctggatg acggtcgaaa atatcgatct caaaaccatt
    22561 ggtttcttct ctctggtagg ccaggcttac gtttttaaat tcctctggtc accgctgatg
    22621 gaccgctaca cgcccccatt ttttgggcgg cggcgcggtt ggctgctcgc cacgcaaatc
    22681 ctgttattag tcgccattgc ggcgatgggt tttctcgaac caggcaccca actccgctgg
    22741 atggcggcgc tggcggtggt gatcgctttt tgctctgcct cccaggatat tgtcttcgat
    22801 gcgtggaaaa ccgatgtgct cccggcagaa gaacgtggtg cgggcgcggc aatcagcgtg
    22861 ctgggttacc gtttagggat gctggtttcc ggcggcctag ccctgtggct ggcagataaa
    22921 tggttgggct ggcagggcat gtactggttg atggcggcgc tgttgatccc ctgtattatc
    22981 acgacgttgc ttgcaccaga accaaccgac accattcctg tgcccaaaac gctggaacaa
    23041 gcggttgttg cacctctgcg agatttcttt ggtcgcaata atgcctggct tattttgctt
    23101 cttatcgtgc tgtataagct gggcgacgca ttcgccatga gcctgacaac cacgtttttg
    23161 attcgcggcg tcgggtttga tgcgggtgaa gtaggcgtgg taaacaaaac gcttggctta
    23221 ctggcgacca ttgttggcgc attgtacggt gggattttga tgcagcgcct gtcgctgttc
    23281 cgggcgctgc tgattttcgg cattttacaa ggtgcgtcta acgctggtta ctggctgctg
    23341 tcgattactg ataagcatct ctacagcatg ggcgcagccg tctttttcga aaacctctgt
    23401 ggcgggatgg gcacatcagc ctttgtcgcg ctgttaatga cgctgtgtaa taagtcattt
    23461 tccgctactc aatttgccct gctctcagcg ctttccgctg tagggcgagt ttatgtcggc
    23521 cccgtggcgg gttggtttgt tgaagcacac ggctggtcga cattctatct attctccgtc
    23581 gccgctgccg taccggggct tattttgctg ctggtttgcc gccagacgct tgaatataca
    23641 cgagtaaatg acaactttat ctcccgtacc gaatatccgg caggttatgc ctttgccatg
    23701 tggacactgg cggcgggcgt cagcctgttg gccgtgtggt tacttctgtt gacgatggac
    23761 gcgctggatt tgacgcactt ctctttcctg cctgctctgc tggaagtcgg ggttttagtc
    23821 gccctttctg gcgtcatgct tggtggtttg ctggattatc tggcgctacg aaaaacgcat
    23881 ctgacgtaat ctgtaaatat tatttagctg tagtaatcac tcgccgtaat tattacggct
    23941 aaataaacat cagcatcgct tattaatatt tgttcttttg tgcggcttag cgtttggaaa
    24001 ttattgacgc catttataaa ttctattttt cttacacgat tcagctaatg agtctttatt
    24061 ttctcaccac ccagttgtca ctttaatgat aattatttgt taaataattg ttttatttcg
    24121 cattggttat accaattgcc cgccaagact ccttctgcac tccccttttg tta
//
LOCUS       DAJTQY010000059        23811 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.059, whole genome shotgun sequence.
ACCESSION   DAJTQY010000059 DAJTQY010000000
VERSION     DAJTQY010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23811
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.059"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            94..1590
                     /gene="ravA"
                     /locus_tag="OO349_002452"
     CDS             94..1590
                     /gene="ravA"
                     /locus_tag="OO349_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HCR5867486.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTLIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSTQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEDSLQDVGAQIRQAQQC"
     gene            1584..3035
                     /gene="viaA"
                     /locus_tag="OO349_002453"
     CDS             1584..3035
                     /gene="viaA"
                     /locus_tag="OO349_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HCR5867487.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDAQVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQFIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVLERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(3040..4032)
                     /gene="asnA"
                     /locus_tag="OO349_002454"
     CDS             complement(3040..4032)
                     /gene="asnA"
                     /locus_tag="OO349_002454"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HCR5867488.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQLSTLKSTVEAIWAGIKATEAAVNEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            4184..4642
                     /gene="asnC"
                     /locus_tag="OO349_002455"
     CDS             4184..4642
                     /gene="asnC"
                     /locus_tag="OO349_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HCR5867489.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            4732..5175
                     /gene="mioC"
                     /locus_tag="OO349_002456"
     CDS             4732..5175
                     /gene="mioC"
                     /locus_tag="OO349_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HCR5867490.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEVGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            5554..7443
                     /gene="mnmG"
                     /locus_tag="OO349_002457"
     CDS             5554..7443
                     /gene="mnmG"
                     /locus_tag="OO349_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HCR5867491.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     RYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASLHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQISGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            7507..8130
                     /gene="rsmG"
                     /locus_tag="OO349_002458"
     CDS             7507..8130
                     /gene="rsmG"
                     /locus_tag="OO349_002458"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HCR5867492.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            8747..9127
                     /gene="atpI"
                     /locus_tag="OO349_002459"
     CDS             8747..9127
                     /gene="atpI"
                     /locus_tag="OO349_002459"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HCR5867493.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            9136..9951
                     /gene="atpB"
                     /locus_tag="OO349_002460"
     CDS             9136..9951
                     /gene="atpB"
                     /locus_tag="OO349_002460"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HCR5867494.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYNIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            9998..10237
                     /gene="atpE"
                     /locus_tag="OO349_002461"
     CDS             9998..10237
                     /gene="atpE"
                     /locus_tag="OO349_002461"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HCR5867495.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            10299..10769
                     /gene="atpF"
                     /locus_tag="OO349_002462"
     CDS             10299..10769
                     /gene="atpF"
                     /locus_tag="OO349_002462"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HCR5867496.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            10784..11317
                     /gene="atpH"
                     /locus_tag="OO349_002463"
     CDS             10784..11317
                     /gene="atpH"
                     /locus_tag="OO349_002463"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HCR5867497.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLTFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            11330..12871
                     /gene="atpA"
                     /locus_tag="OO349_002464"
     CDS             11330..12871
                     /gene="atpA"
                     /locus_tag="OO349_002464"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HCR5867498.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            12922..13785
                     /gene="atpG"
                     /locus_tag="OO349_002465"
     CDS             12922..13785
                     /gene="atpG"
                     /locus_tag="OO349_002465"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HCR5867499.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            13812..15194
                     /gene="atpD"
                     /locus_tag="OO349_002466"
     CDS             13812..15194
                     /gene="atpD"
                     /locus_tag="OO349_002466"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HCR5867500.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            15215..15634
                     /gene="atpC"
                     /locus_tag="OO349_002467"
     CDS             15215..15634
                     /gene="atpC"
                     /locus_tag="OO349_002467"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HCR5867501.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            15985..17355
                     /gene="glmU"
                     /locus_tag="OO349_002468"
     CDS             15985..17355
                     /gene="glmU"
                     /locus_tag="OO349_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HCR5867502.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGTKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            17517..19346
                     /gene="glmS"
                     /locus_tag="OO349_002469"
     CDS             17517..19346
                     /gene="glmS"
                     /locus_tag="OO349_002469"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HCR5867503.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            19506..20327
                     /locus_tag="OO349_002470"
     CDS             19506..20327
                     /locus_tag="OO349_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heteromeric transposase endonuclease subunit
                     TnsA"
                     /protein_id="HCR5867504.1"
                     /translation="MAKANSSFSEVQIARRIKEGRGQGHGKDYIPWLTVQEVPSSGRS
                     HRIYSHKTGRVHHLLSDLELAVFLSLEWESSVLDIREQFPLLPSDTRQIAIDSGIKHP
                     VIRGVDQVMSTDFLVDCKDGPFEQFAIQVKPAAALQDERTLEKLELERRYWQQKQIPW
                     FIFTDKEINPVVKENIEWLYSVKTEEVSAELLAQLSPLAHILQEKGDENIINVCKQVD
                     IAYDLELGKTLSEIRALTANGFIKFNIYKSFRANKCADLCISQVVNMEELRYVAN"
     gene            20314..22422
                     /locus_tag="OO349_002471"
     CDS             20314..22422
                     /locus_tag="OO349_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000267723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HCR5867505.1"
                     /translation="MWQINEVVLFDNDPYRILAIEDGQVVWMQISADKGVPQARAELL
                     LMQYLDEGRLVRTDDPYVHLDLEEPSVDSVSFQKREEDYRKILPIINSKDRFDPKVRS
                     ELVEHVVQEHKVTKATVYKLLRRYWQRGQTPNALIPDYKNSGAPGERRSATGTAKIGR
                     AREYGKGEGTKVTPEIERLFRLTIEKHLLNQKGTKTTVAYRRFVDLFAQYFPRIPQED
                     YPTLRQFRYFYDREYPKAQRLKSRVKAGVYKKDVRPLSSTATSQALGPGSRYEIDATI
                     ADIYLVDHHDRQKIIGRPTLYIVIDVFSRMITGFYIGFENPSYVVAMQAFVNACSDKT
                     AICAQHDIEISSSDWPCVGLPDVLLADRGELMSHQVEALVSSFNVRVESAPPRRGDAK
                     GIVESTFRTLQAEFKSFAPGIVEGSRIKSHGETDYRLDASLSVFEFTQIILRTILFRN
                     NHLVMDKYDRDADFPTDLPSIPVQLWQWGMQHRTGSLRAVEQEQLRVALLPRRKVSIS
                     SFGVNLWGLYYSGSEILREGWLQRSTDIARPQHLEAAYDPVLVDTIYLFPQVGSRVFW
                     RCNLTERSRQFKGLSFWEVWDIQAQEKHNKANAKQDELTKRRELEAFIQQTIQKANKL
                     TPSTTEPKSTRIKQIKTNKKEAVTSERKKRAEHLKPSSSGDEAKVIPFNAVEADDQED
                     YSLPTYVPELFQDPPEKDES"
     gene            22419..>23811
                     /locus_tag="OO349_002472"
     CDS             22419..>23811
                     /locus_tag="OO349_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR5867506.1"
                     /translation="MSATRIQAVYRDTGVEAYRDNPFIEALPPLQESVNSAASLKSSL
                     QLTSSDLQKSRVIRAHTICRIPDDYFQPLGTHLLLSERISVMIRGGYVGRNPKTGDLQ
                     KHLQNGYERVQTGELETFRFEEARSTAQSLLLIGCSGSGKTTSLHRILATYPQVIYHR
                     ELNVEQVVYLKIDCSHNGSLKEICLNFFRALDRALGSNYERRYGLKRHGIETMLALMS
                     QIANAHALGLLVIDEIQHLSRSRSGGSQEMLNFFVTMVNIIGVPVMLIGTPKAREIFE
                     ADLRSARRGAGFGAIFWDPIQQTQRGKPNQEWIAFTDNLWQLQLLQRKDALLSDEVRD
                     VWYELSQGVMDIVVKLFVLAQLRALALGNERITAGLLRQVYQDELKPVHPMLEALRSG
                     IPERIARYSDLVVPEIDKRLIQLQLDIAAIQEQTPEEKALQELDTEDQRHLYLMLKED
                     YDSSLLIPTIKKAF"
BASE COUNT         6047 a         5694 c         6188 g         5882 t
ORIGIN      
        1 attacccccc tttgagacta ttttagtcac cagcaagaaa cgtctatact cgcaatttac
       61 gcagaacttt tgacgaaagg acgccacttc attatggctc accctcattt attagcggaa
      121 agaatttccc gcctgagcag ttcgttggaa aaggggcttt atgaacgtag ccacgccatc
      181 cgcttgtgtt tattagcggc attaagtggt gaaagtgtgt tcctccttgg cccgccaggt
      241 attgccaaaa gtttgatcgc ccggcgctta aaattcgcct ttcagaatgc ccgcgcgttt
      301 gaatatctga tgacccgctt ctccacgccg gaagaagttt ttggtcccct ttctattcag
      361 gcgctaaaag atgaagggcg ctatgaacgt ttaaccagcg gttacctgcc ggaagcagaa
      421 atcgtctttc tggatgagat ctggaaagcg ggcccggcaa ttcttaatac cttgctcacc
      481 gccattaacg agcgccagtt ccgcaacggt gcacacgtag aaaaaatccc gatgcgtcta
      541 ctggtggcgg cctccaacga gctgccggaa gcagacagca gtctggaagc gttatatgac
      601 cgcatgctga ttcgtctatg gttagataaa gtgcaagata aagcgaattt ccgctccatg
      661 ctgaccagtc aacaggatga aaacgacaat ccggttcctg atgccttgca ggtcacagat
      721 gaagaatatg aacgctggca gaaagagatt ggtgaaatta cgctgcccga tcatgtattt
      781 gagctgattt ttatgctgcg ccagcaactg gataaattac cggatgcgcc ttatgtctcg
      841 gatcgtcgct ggaaaaaagc gatccgctta ttgcaggcca gcgccttttt tagcggtcgc
      901 agtgctgttg ccccggttga tctcattttg ctgaaagatt gcctgtggta tgacgcgcaa
      961 agcctgaatt tgatacaaca acaaattgat gtattgatga ccggtcatgc ctggcaacag
     1021 caagggatgt tgacccgcct gggcgcgatt gtgcaacgtc acctgcaact ccagcagcaa
     1081 caaagcgata aaacagcctt aacgttaatt cgtctgggcg gcattttcag ccgtcgtcag
     1141 cagtatcaac ttcctgttaa cgttactgct tccactctga ctctgctgct gcaaaaaccg
     1201 ttaaaactgc atgatatgga agtggttcat atctcctttg agcgtagcgc gctggaacag
     1261 tggctgagta aaggtggtga aattcgcggc aaactgaacg gtatcggctt tgcccagaaa
     1321 ctgaatctgg aagttgatag cacccaacat cttgttgtac gcgatgtaag tttacaaggc
     1381 agtacgctgg cacttcccgg ttcatcggct gaaggtctgc caggtgaaat aaaacaacaa
     1441 ctggaagagc ttgaaagcga ctggcgtaag caacacgctt tattcagcga acagcaaaaa
     1501 tgtctgttta tccctggcga ctggttaggt cgcattgaag acagcctaca ggatgtcggt
     1561 gcacagattc gtcaggcaca acaatgctaa cgctggatac gcttaatgtg atgctggccg
     1621 tcagcgaaga gggattgatc gaagagatga tcatcgcact gctggcctca ccgcagctgg
     1681 cggtcttctt tgaaaaattc ccacgactga aggcggcaat cactgatgat gttccccgct
     1741 ggcgtgaggc gctgcgcagt cggctgaaag atgcccaagt cccgccggaa ctcaccgaag
     1801 aggtgatgtg ctatcagcaa agccagctcc tctccacgcc gcagtttatt gtgcagctac
     1861 cacagatcct ggacttactg catcgtctga attccccatg ggcagaacaa gcccggcagt
     1921 tggttgatgc taacagcaca ataacttcag cgttacacac gctttttctc cagcgttggc
     1981 gtttaagtct gatcgtgcaa gcaacgacgt taaatcaaca gctattagaa gaagaacgcg
     2041 aacaactgtt aagtgaagtt caggaacgca tgacgctgag cggacaactt gaaccgattc
     2101 tcgcagataa caataccgca gctggtcgtc tgtgggatat gagcgccggt caacttaaac
     2161 gtggcgacta tcagtttatt gtgaaatacg gtgaatttct taacgaacag ccggaactga
     2221 aacgcctggc agagcagctg gggcgttctc gggaagccaa atcaataccg cgcaacgatg
     2281 cgcagatgga aaccttccgc accatggtgc gcgaaccggc gacggttcct gagcaggttg
     2341 atggtctgca acaaagcgat gatattttac gtctcctgcc gccagaactg gcgacactag
     2401 ggataacgga actggagtat gagttttacc gtcggctggt ggaaaaacag ttgctcacct
     2461 atcgcctgca cggtgagtcg tggcgtgaaa aagtgctcga acgtccggtg gtacataaag
     2521 attacgatga acagccgcgc gggccgttta ttgtctgtgt ggatacttcc ggctcaatgg
     2581 gcggctttaa tgaacagtgt gcgaaagcgt tctgcctggc tttgatgcgc attgctctcg
     2641 ctgaaaaccg gcgctgttat attatgctat tttccaccga gatcgtccgt tatgagcttt
     2701 caggcccaca aggcatcgaa caagcaatcc gttttttaag ccagcagttt cgtggcggca
     2761 ccgatcttgc cagttgtttt cgcgccatta tggaacgctt gcaaagcagg gaatggtttg
     2821 atgccgatgc ggtggtgatt tctgatttta tcgctcagcg gttgcctgac gacgtgacga
     2881 gtaaagtgaa agagctgcag cgggtacatc agcatcgctt tcatgccgtg gcgatgtcgg
     2941 cacacggcaa acccggcatc atgcgcattt tcgatcatat ctggcgcttt gataccggga
     3001 tgcgaagccg cctgctcaga cgctggcggc gataaattat tacagcagag aagggacgct
     3061 ctcgcgaaca gcagctggcc atactccaca ctgaacctgg ccgatatgcg gcagttgcag
     3121 cagcagcata gtcaaacgag actggccgat accgccgccg atggtctgcg gcatttcacc
     3181 gcgcagcagc gcctgatgcc attccagctg cagacgatct tcatcaccgg tcagcgccag
     3241 ctgatgcttc agcgtgtcgg catccacgcg gatccccatg gaagaaagct caaacgcatc
     3301 ttccagtacc gggttccaca ccagaatatc gccgttcagg cccgcatggc ccagctctga
     3361 cggggtgctc cagtcatcat aatccggtgc gcgcacgtcg tgacgatgac catcactcaa
     3421 cttgccgcca atcccgacga ggaataccgc gccaagatct ttcgcaatcg cccgctcacg
     3481 ccctttggca tcgagatccg gataacgaga cagtaactcc tggctgtgta cgaagtgaat
     3541 ctgatccggc aggaacggtg ccaggccaaa ctcttcgtta accgcagctt cggttgcttt
     3601 aattcccgcc cagatcgcct ctaccgtgct tttcagagtc gagagttgac gctcaccgtc
     3661 gcccattacg cgttcccagt cccactggtc aacatagacc gagtgcaacg gagaaagacg
     3721 gtcttcatcg gggcgaaggg ctttcatgtg cgtgtacagc ccttcgcccg cgctgaagtc
     3781 gtgttgccct aaggtctgac gtttccactt cgccagtgaa tgaaccactt cgaactgggc
     3841 atcaggcaga gctttcactt ttacctgcac cgctttttca cagcccgaca agttatcctg
     3901 cgtgccatcc cccacacggc taagaatcgg cgcctggact tcgatcagcc ccagacgttc
     3961 ttccagttgg cgagaaaagt gagatttcac gaagctaatt tggcgttgtt tggcaatgta
     4021 agcggttttc attttttata ctcctgcgtc ctgttgctta tgattaagca acaaaaaccg
     4081 acacacatgc aataatcatt caataaaaag cctaacacac ttttgattca ttaaaaaaga
     4141 cggctaaaat agaatgaatc atcaatctgc ataagaaaat cctatggaaa attatctgat
     4201 cgacaatctg gaccgtggca tcctggaagc attaatgggc aatgcgcgca ccgcttacgc
     4261 cgaactggcg aaacaatttg gcgtcagtcc ggggacgatt cacgttcgag tagagaaaat
     4321 gaagcaggcg gggatcatta ccggggcgcg tattgatgtc agcccgaagc agctcggtta
     4381 tgacgtaggc tgctttatcg gcattatatt aaagagcgcc aaagactacc cttccgcgct
     4441 ggcaaagctg gaaagccttg atgaagtcac tgaagcctat tacacaaccg gccactacag
     4501 catctttata aaagttatgt gccgttcgat cgacgctctc cagcatgtac ttatcaacaa
     4561 gatccaaaca attgatgaaa ttcagtccac cgagacattg atcgtcctgc aaaacccgat
     4621 catgcgtacc atcaagccct gatcggcttt tttaatccca tacttttcca caggtagatc
     4681 ccaacgcgtt cacagcgtac aatacgccac tcttaataaa ggtggcggtt tatggcagat
     4741 atcactctta tcagcggcag caccctcggc ggtgccgaat atgtagcaga acacctggct
     4801 gaaaagctgg aagaggtggg ttttaccacc gaaacgctgc acggtccgct gttggaagat
     4861 ttatctgcct cggggatctg gctggttatc agctccaccc acggtgccgg agatattccg
     4921 gacaaccttt ctcctttcta tgaagcattg caggaacaga agcccgatct ttctgcggtc
     4981 cgctttggcg caatcggtat tggcagtcgt gaatatgaca ccttttgtgg ggctatcgat
     5041 aaactcgagg cagaactcaa aaattccggt gcaaaacaga caggcgaaac actgaagatc
     5101 aacattcttg atcacgacat tccggaagat ccggcagaag aatggctggg atcgtggatt
     5161 aatttactca aataagtata cagatcgtgc gatctactgt ggataactct gtcaggaagc
     5221 ttggatcaac cggtagctat ccaaagaaca accgttgttc agtttttgag ttgtgtataa
     5281 cccctcattc tgatcccagc ttatacggtc caggatcacc gatcattcac agttaatgat
     5341 cctttccagg ttgttgatct taaaaatcgg atccttgtta tccacagggc agtgcgatcc
     5401 taataagaga tcacaataga acagatctct aaataaatag atcttctttt taatacccag
     5461 gatcccaggt ctttctcaag ccgacaaagt tgagtagaat ccacggcccg ggcttcaatc
     5521 cattttcata ccgcgttatg cgaggcaatc accatgtttt atccggatcc ttttgacgtc
     5581 atcatcattg gcgggggtca tgcaggcacc gaggccgcga tggctgcggc gcgtatgggt
     5641 caacagactc tgcttttgac acacaatatc gacactctgg ggcagatgag ctgcaacccg
     5701 gcgatcggcg gtattgggaa gggacatctg gtaaaagaag tggatgcact cggcggtctg
     5761 atggcgaaag cgatcgatca agcgggtatc cagtttagga tactaaacgc aagcaaagga
     5821 ccggcggttc gcgctacccg agctcaggcg gatcgtgtgc gctaccgtca ggcggtacgt
     5881 acggcgctgg agaaccaacc gaacctgatg atcttccagc aggcggttga agatcttatt
     5941 gtcgaaaacg atcgcgtggt cggtgctgtt acccaaatgg gactgaagtt ccgtgccaaa
     6001 gctgtggtgc tcaccgttgg gacgttcctc gacggcaaaa ttcatatcgg tctggataat
     6061 tacagcggtg gccgtgctgg cgatccgcca tccattccgc tctctcgccg tttgcgtgaa
     6121 ctgccgctgc gcgttggtcg tctgaaaacc ggtactccgc cgcgtattga tgctcgaacc
     6181 atcgacttta gcgtgctggc gcaacagcat ggcgataacc caatgccggt attctcgttt
     6241 atgggcaatg cgtccctgca tccccagcag gtgccgtgtt atatcactca taccaacgag
     6301 aaaacccatg atgtgatccg cagtaacctc gatcgtagcc caatgtatgc aggggtgatc
     6361 gaaggtgtcg gcccacgcta ctgcccgtcg atcgaagaca aagtcatgcg cttcgcagac
     6421 agaaatcagc atcagatctt ccttgaaccg gaaggactga cctctaatga aatttatccg
     6481 aacggtatct ccaccagcct gccgttcgat gtgcagatgc aaatcgtccg ctctatgcag
     6541 gggatggaaa acgcgaagat cgtgcgtccg ggttatgcca ttgagtatga cttcttcgat
     6601 cctcgcgacc tgaaaccgac gctggagagc aagtttatcc aggggctgtt ctttgctggt
     6661 cagattaacg gcactaccgg ttacgaagaa gccgctgcgc aaggtttgct ggccggtctt
     6721 aacgctgccc gtctgtctgc tgacaaagaa ggttgggctc cggcacgttc tcaggcgtat
     6781 ctcggcgtac tggttgatga cctgtgcact ttaggaacca aagaaccgta tcgtatgttt
     6841 acttcgcgcg cagaatatcg tctgatgctg cgcgaagata atgcggatct gcgtttgact
     6901 gaaatcggtc gtgaactggg cctggtggat gacgaacgtt gggcgcgctt taacgagaaa
     6961 cttgagaata tcgagcgtga gcgtcagcgt ctgaaatcga cctgggtaac cccgtcggcg
     7021 gaagctgcag ccgaagtgaa tgctcacctg actgcgccgc tttcccgtga agccagtggt
     7081 gaagatctgc tgcgtcgtcc ggaaatgact tatgaaaaat taaccacgct gacgccgttt
     7141 gcccctgcgt tgacagacga acaggcggcg gaacaggttg agattcaggt taaatacgaa
     7201 ggttatatcg cgcgccagca agatgagatc gaaaagcagc tgcgtaacga gaacaccctg
     7261 ctacccgcga cactggatta ccgccagata tccggtcttt ctaacgaagt gatcgccaaa
     7321 cttaacgatc acaaaccggc ctctatcggc caggcttcgc gtatttctgg cgtcacgcct
     7381 gcggccatct ccattctgct ggtgtggctg aaaaaacagg gtatgctgcg tcgtagcgca
     7441 taacgcatta aaaatgcctg gtaagcaccc gcttaccagg caacgcatca agaacaggta
     7501 atcaccgtgc tcaacaaact ctccttactg ctgaaagacg caggtatttc gcttactgat
     7561 caccagaaaa accagcttat tgcctacgtg aatatgctgc ataaatggaa caaagcgtac
     7621 aacctgactt cggtccgcga tcctaatgag atgctggtac gccatattct cgatagcatt
     7681 gtggtggcac cgtatctgca aggtgaacgg tttatcgatg tcggcaccgg accaggactg
     7741 ccaggcattc cactctctat cgtgcgtcct gaagcccatt tcactctgtt ggatagcctt
     7801 ggtaaacgcg tgcgtttcct tcgtcaggtg caacatgagc ttaaactgga gaatattgaa
     7861 ccagtacaga gcagggtaga agagtttcct tcagagccgc catttgatgg cgtaattagc
     7921 cgcgcttttg cctctctgaa cgatatggtg agctggtgcc accatcttcc tggtgagcaa
     7981 ggccgtttct acgcgctgaa agggcaaatg ccggaagatg aaatcgcttt gttgcccgaa
     8041 gaatatcagg tcgaatcagt ggttaaactt caggttccag ccctggatgg cgaacgtcat
     8101 ctggtggtga ttaaagcaaa taaaatttaa tttttatcaa aaaaatcata aaaaattgac
     8161 cggttagact gttaacaaca accaagtttt ctactgatat aactggttac atttaacgcc
     8221 acgttcactc ttttgcatca acaagataac gtggcttttt ttggtaagca gaaaataagt
     8281 cattagtgaa aatatcagtc tgctaaaaat cggcgctaag aaccatcatt ggctgttaaa
     8341 acattattaa aaatgtcaat gggtggtttt tgttgtgtaa atgttattta ttaaaacagt
     8401 atctgttttt agactgaaat atcataaact tgtaaaggca tcatttgcca agtaaataaa
     8461 tatgctgtgc gcgaacatgc gcaatatgtg atctgaagca cgctttatca ccagtgttta
     8521 cgcgttattt acagtttttc atgatcgaac aggggtagcg gaaaagtcgc aattgtatgc
     8581 actggaaaaa tatttaaaca tttattcacc ttttggctac ttattgtttg aaatcacggg
     8641 ggcgcaccgt ataatttgac cgctttttga tgcttgactc taagccttaa agaaagtttt
     8701 atacgacacg cggcatacct cgaagggagc aggagtgaaa aacgtgatgt ctgtgtcgct
     8761 cgtgagtcga aacgttgctc ggaagcttct gctcgttcag ttactggtgg tgatagcaag
     8821 tggattgctg ttcagcctca aagacccctt ctggggcgtc tctgcaataa gcgggggcct
     8881 ggcagtcttt ctgcctaacg ttttgtttat gatatttgcc tggcgtcacc aggcgcatac
     8941 accagcgaaa ggccgggtgg cttggacatt cgcatttggc gaagctttca aagttctggc
     9001 gatgttggtg ttactggtgg tggcgttggc ggttttaaag gcggtattct tgccgctgat
     9061 cgttacgtgg gttttggtgc tggtggttca gatactggca ccggctgtaa ttaacaacaa
     9121 agggtaaaag gcatcatggc ttcagaaaat atgacgccgc aggattacat aggacaccac
     9181 ctgaataacc ttcagctgga cctgcgtaca ttctcgctgg tggatccaca aaacccccca
     9241 gccaccttct ggacaatcaa tattgactcc atgttcttct cggtggtgct gggtctgttg
     9301 ttcctggttt tattccgtag cgtagccaaa aaggcgacca gcggtgtgcc aggtaagttt
     9361 cagaccgcga ttgagctggt gatcggcttt gttaatggta gcgtgaaaga catgtaccat
     9421 ggcaaaagca agctgattgc tccgctggcc ctgacgatct tcgtctgggt attcctgatg
     9481 aatctgatgg atttactgcc tatcgacttg ctgccgtaca ttgctgaaca tgtactgggt
     9541 ctgcctgcac tgcgtgtggt tccgtctgcg gacgtgaacg taacgctgtc tatggcactg
     9601 ggcgtattta tcctgattct gttctacaac atcaaaatga aaggcatcgg cggcttcacg
     9661 aaagagttga cgctgcagcc gttcaatcac tgggcgttca ttcctgtcaa cttaatcctt
     9721 gaaggggtaa gcctgctgtc caaaccagtt tcactcggtt tgcgactgtt cggtaacatg
     9781 tatgccggtg agctgatttt cattctgatt gctggtctgt tgccgtggtg gtcacagtgg
     9841 atcctgaatg tgccgtgggc cattttccac atcctgatca ttacgctgca agccttcatc
     9901 ttcatggttc tgacgatcgt ctatctgtcg atggcgtctg aagaacatta atttaccaac
     9961 actactacgt tttaactgaa acaaactgga gactgtcatg gaaaacctga atatggatct
    10021 gctgtacatg gctgccgctg tgatgatggg tctggcggca atcggtgctg cgatcggtat
    10081 cggcatcctc gggggtaaat tcctggaagg cgcagcgcgt caacctgatc tgattcctct
    10141 gctgcgtact cagttcttta tcgttatggg tctggtggat gctatcccga tgatcgctgt
    10201 aggtctgggt ctgtacgtga tgttcgctgt cgcgtagtaa gcgttgcttt tatttaaaga
    10261 gcaatatcag aacgttaact aaatagaggc attgtgctgt gaatcttaac gcaacaatcc
    10321 tcggccaggc catcgcgttt gtcctgttcg ttctgttctg catgaagtac gtatggccgc
    10381 cattaatggc agccatcgaa aaacgtcaaa aagaaattgc tgacggcctt gcttccgcag
    10441 aacgagcaca taaggacctt gaccttgcaa aggccagcgc gaccgaccag ctgaaaaaag
    10501 cgaaagcgga agcccaggta atcatcgagc aggcgaacaa acgccgctcg cagattctgg
    10561 acgaagcgaa agctgaggca gaacaggaac gtactaaaat cgtggcccag gcgcaggcag
    10621 aaattgaagc cgagcgtaaa cgtgcccgtg aagagctgcg taagcaagtt gctatcctgg
    10681 ctgttgctgg cgccgagaag atcatcgaac gttccgtgga tgaagctgct aacagcgaca
    10741 tcgtggataa acttgtcgct gaactgtaag gagggagggg ctgatgtctg aatttattac
    10801 ggtagctcgc ccctacgcca aagcagcttt tgactttgcc gtcgaacacc aaagtgtaga
    10861 acgctggcag gacatgctga cgtttgccgc cgaggtaacc aaaaacgaac aaatggcaga
    10921 gcttctctct ggcgcgcttg cgccagaaac gctcgccgag tcgtttatcg cagtttgtgg
    10981 tgagcaactg gacgaaaacg gtcagaacct gattcgggta atggctgaaa atggtcgtct
    11041 taacgcgctc ccggatgttc tggagcagtt tattcacctt cgtgccgtga gtgaggctac
    11101 cgctgaggta gacgtcattt ccgctgccgc actgagtgaa caacagctcg cgaaaatttc
    11161 tgctgcgatg gaaaaacgtc tgtcacgcaa agttaagctg aattgcaaaa tcgataagtc
    11221 tgtaatggca ggcgttatca tccgagcggg tgatatggtc attgatggca gcgtacgcgg
    11281 tcgtcttgag cgccttgcag acgtcttgca gtcttaaggg gactggagca tgcaactgaa
    11341 ttccaccgaa atcagcgaac tgatcaagca gcgcattgct cagttcaatg ttgtgagtga
    11401 agctcacaac gaaggtacta ttgtttctgt aagtgacggt gttatccgca ttcacggcct
    11461 ggccgattgt atgcagggtg aaatgatctc cctgccgggt aaccgttacg ctatcgcact
    11521 gaacctcgag cgcgactctg taggtgcggt tgttatgggt ccgtacgctg accttgccga
    11581 aggcatgaaa gttaagtgta ctggccgtat cctggaagtt ccggttggcc gtggcctgct
    11641 gggccgtgtg gttaacactc tgggtgcacc aatcgacggt aaaggtccgc tggatcacga
    11701 cggcttctct gctgtagaag caatcgctcc gggcgttatc gaacgtcagt ccgtagatca
    11761 gccggtacag accggttata aagccgttga ctccatgatc ccaatcggtc gtggtcagcg
    11821 tgaattgatc atcggtgacc gtcagacagg taaaaccgca ctggctatcg atgccatcat
    11881 caaccagcgc gattccggta tcaaatgtat ctatgtcgct atcggccaga aagcgtccac
    11941 catttctaac gtggtacgta aactggaaga gcacggcgca ctggctaaca ccatcgttgt
    12001 ggtagcaacc gcgtctgaat ccgctgcact gcaatacctg gcaccgtatg ccggttgcgc
    12061 aatgggcgaa tacttccgtg accgcggtga agatgcgctg atcatttacg atgacctgtc
    12121 taaacaggct gttgcttacc gtcagatctc cctgctgctc cgtcgtccgc caggacgtga
    12181 agcattcccg ggcgacgttt tctacctcca ctctcgtctg ctggagcgtg ctgcacgtgt
    12241 taacgccgaa tacgttgaag ccttcaccaa aggtgaagtg aaagggaaaa ccggttctct
    12301 gaccgcactg ccgattatcg aaactcaggc gggtgacgtt tctgcgttcg ttccgaccaa
    12361 cgtaatctcc attaccgatg gtcagatctt cctggaaacc aacctgttca acgccggtat
    12421 tcgtcctgcg gttaacccgg gtatttccgt atcccgtgtt ggtggtgcag cacagaccaa
    12481 gatcatgaaa aaactgtccg gtggtatccg taccgctctg gcacagtatc gtgaactggc
    12541 agcgttctct cagtttgcat ccgaccttga cgatgcaaca cgtaagcagc ttgaccacgg
    12601 tcagaaagtg accgaactgc tgaaacagaa acagtatgcg ccgatgtccg ttgcgcagca
    12661 gtctctggtt ctgttcgcag cagaacgtgg ttacctggcg gatgttgaac tgtcgaaaat
    12721 tggcagcttc gaagccgctc tgctggctta cgtcgaccgt gatcacgctc cgttgatgca
    12781 agagatcaac cagaccggtg gctacaacga cgaaatcgaa ggcaagctga aaggcatcct
    12841 cgattccttc aaagcaaccc aatcctggta acgtctggcg gcttgcctta gggcaggccg
    12901 caaggcattg aggagaagct catggccggc gcaaaagaga tacgtagtaa gatcgcaagc
    12961 gtccagaaca cgcaaaagat cactaaagcg atggagatgg tcgccgcttc caaaatgcgt
    13021 aaatcgcagg atcgcatggc ggccagccgt ccttatgcag aaaccatgcg caaagtgatt
    13081 ggtcaccttg cacacggtaa tctggaatat aagcaccctt acctggaaga ccgcgacgtt
    13141 aaacgcgtgg gctacctggt ggtgtcgacc gaccgtggtt tgtgcggtgg tttgaacatt
    13201 aacctgttca aaaaactgct ggcggaaatg aagacctgga ccgacaaagg cgttcaatgc
    13261 gacctcgcaa tgatcggctc gaaaggcgtg tcgttcttca actccgtggg cggcaatgtt
    13321 gttgcccagg tcaccggcat gggggataac ccttccctgt ccgaactgat cggtccggta
    13381 aaagtgatgt tgcaggccta cgacgaaggc cgtctggaca agctttacat tgtcagcaac
    13441 aaatttatta acaccatgtc tcaggttccg accatcagcc agctgctgcc gttaccggca
    13501 tcagatgatg atgatctgaa acataaatcc tgggattacc tgtacgaacc cgatccgaag
    13561 gcgttgctgg ataccctgct gcgtcgttat gtcgaatctc aggtttatca gggcgtggtt
    13621 gaaaacctgg ccagcgagca ggccgcccgt atggtggcga tgaaagccgc gaccgacaat
    13681 ggcggcagcc tgattaaaga gctgcagttg gtatacaaca aagctcgtca ggccagcatt
    13741 actcaggaac tcaccgagat cgtctcgggg gccgccgcgg tttaaacagg ttatttcgta
    13801 gaggatttaa gatggctact ggaaagattg tccaggtaat cggcgctgta gttgacgtcg
    13861 aattccctca ggatgccgta ccgcgcgtgt acgatgctct tgaggtgcaa aatggtaatg
    13921 agcgtctggt gctggaagtt cagcagcagc tcggcggcgg tatcgtgcgt accatcgcaa
    13981 tgggttcctc cgacggtctg cgtcgcggtc tggatgtaaa agacctcgaa cacccgatcg
    14041 aagtcccggt aggtaaagcg actctgggcc gtatcatgaa cgtactgggt gaaccggtcg
    14101 acatgaaagg cgagatcggt gaagaagagc gttgggcgat tcaccgcgca gcaccttcct
    14161 acgaagagct gtcaaactct caggaactgc tggaaaccgg tatcaaagtt atcgacctga
    14221 tgtgtccgtt cgctaagggc ggtaaagttg gtctgttcgg tggtgcgggt gtaggtaaaa
    14281 ccgtaaacat gatggagctc attcgtaaca tcgcgatcga gcactccggt tactctgtgt
    14341 ttgcgggcgt aggtgaacgt actcgtgagg gtaacgactt ctaccacgaa atgaccgact
    14401 ccaacgttat cgacaaagta tccctggtgt atggccagat gaacgagccg ccgggaaacc
    14461 gtctgcgcgt tgctctgacc ggtctgacca tggctgagaa attccgtgac gaaggtcgtg
    14521 acgttctgct gttcgttgac aacatctatc gttacaccct ggccggtacg gaagtatccg
    14581 cactgctggg ccgtatgcct tcagcggtag gttatcagcc gaccctggcg gaagagatgg
    14641 gcgttctgca ggaacgtatc acctccacca aaactggttc tatcacctcc gtacaggcgg
    14701 tatacgtacc tgcggatgac ttgactgacc cgtctccggc aaccaccttt gcgcaccttg
    14761 acgcaaccgt ggtactgagc cgtcagatcg cgtctctggg tatctacccg gccgttgacc
    14821 cgctggactc caccagccgt cagctggacc cgctggtggt tggtcaggaa cactacgaca
    14881 ccgcgcgtgg cgttcagtcc atcctgcaac gttatcagga actgaaagac atcatcgcca
    14941 tcctgggtat ggatgaactg tctgaagaag acaaactggt ggtagcgcgt gctcgtaaga
    15001 tccagcgctt cctgtcccag ccgttcttcg tggcagaagt attcaccggt tctccgggta
    15061 aatacgtctc cctgaaagac accatccgtg gctttaaagg catcatggaa ggcgaatacg
    15121 atcacctgcc ggagcaggcg ttctacatgg tcggttccat cgaagaagct gtggaaaaag
    15181 ccaaaaaact ttaacgcctt aatcggaggg tgatatggca atgacttacc acctggacgt
    15241 cgtcagcgca gagcaacaaa tgttctctgg tctggtcgag aaaatccagg taacgggtag
    15301 cgaaggtgaa ctggggatct accctggcca cgcaccgctg ctcaccgcca ttaagcctgg
    15361 tatgattcgc atcgtgaaac agcacggtca cgaagagttt atctatctgt ctggcggcat
    15421 tcttgaagtg cagcctggca acgtgaccgt tctggccgac accgcaattc gcggccagga
    15481 tctcgacgaa gcgcgagcca tggaagcgaa acgtaaggct gaagagcaca ttagcagctc
    15541 tcacggcgac gtagattacg ctcaggcgtc tgcggaactg gccaaagcga tcgcgcagct
    15601 gcgcgttatc gagttgacca aaaaagcgat gtaacaccgg cttgaaaagc acaaaagcca
    15661 gtctggaaac aggctggctt tttttacgcg ttgacccgtc ctgaagagcg ttcacataga
    15721 tcctgctgat ataaaacacc cctgttttcc cgtttattca ttgatcgaaa taagagcaaa
    15781 aacatccacc tgacgcttaa atgaaggtac tgccttaatt ttctgcagac aaaaggcgtg
    15841 acgatggtcg aaaatggcac tttcgtcagc ggggataatc cgttattgaa caatttatcc
    15901 tctgtccatt tcacgatgaa aaaaatgtag ttttttcaag gtgaagcggt ttaaattcgt
    15961 tctcaaatta cagtcaggac gcgtatgttg aataatgcta tgagcgtagt gatccttgcc
    16021 gcaggcaaag gcacgcgcat gtattccgat cttccgaaag tgctgcatac ccttgccggg
    16081 aaagcgatgg ttcagcatgt cattgatgct gcgaatgaat taggcgcagc gcacgttcac
    16141 ctggtgtacg gtcacggcgg cgatctgctt aaacaggcgc tgaaagacga caaccttaac
    16201 tgggtgcttc aggcagagca gctgggtacg ggtcatgcaa tgcagcaggc cgcacctttc
    16261 tttgccgatg atgaagacat tttaatgctc tacggcgacg tgccgctgat ctctgtcgaa
    16321 acactccagc gcctgcgtga tgctaaaccc cagggtggca ttggtctgct gacggtgaaa
    16381 ctggatgatc cgaccggtta tggacgtatc acccgtgaaa atggcaaagt taccggcatt
    16441 gttgagcaca aagatgccac cgacgagcag cgtcagattc aggagatcaa caccggcatt
    16501 ctgattgcca acggcgcaga tatgaaacgc tggctggcga agctgaccaa caataatgct
    16561 cagggcgaat actacatcac cgacattatt gcgctggcgt atcaggaagg gcgtgaaatc
    16621 gtcgccgttc atccgcaacg tttaagcgaa gtagaaggtg tgaataaccg cctgcaactc
    16681 tcccgtctgg agcgcgttta ccagtccgaa caggctgaaa aactgctgtt agcaggcgtt
    16741 atgctgcgcg atccggcgcg ttttgatctg cgcggtacgc ttactcacgg gcgcgatgtt
    16801 gaaattgata ctaacgttat catcgagggc aacgtgactc tcggtcatcg cgtgaaaatc
    16861 ggcaccggtt gcgtgattaa aaacagcgtg attggcgatg attgcgaaat cagtccgtat
    16921 accgttgtgg aagacgcgaa tctggcagcg gcctgtacta ttggcccgtt tgcccgtctg
    16981 cgtcctggtg ctgagttgct ggaaggtgca cacgttggta acttcgttga gatgaaaaaa
    17041 gcgcgtctgg gtaaaggcac gaaagccggg catctgactt acctgggcga tgcggaaatt
    17101 ggcgataacg ttaacatcgg cgcgggaacc attacctgca actacgatgg tgcgaataaa
    17161 tttaagacca ttatcggcga cgatgtgttt gtcggttccg acactcagct ggtggccccg
    17221 gtaacagtag gcaaaggcgc gaccattgct gcgggtacaa ctgtgacgcg taatgtcggc
    17281 gaaaatgcat tagctatcag ccgtgtgccg cagactcaga aagaaggctg gcgtcgtccg
    17341 gtaaagaaaa agtaattctg gccgggtaat ccggtcatct gggatgagga gataacataa
    17401 tctccctccc acaagcagta actataaaaa taaccccact ctctacaagg ctcggggcgc
    17461 ccgaaaaaac gggcatacag gttgaccgac aacgatataa atcggaatca aaaactatgt
    17521 gtggaattgt tggcgcgatc gcgcaacgtg atgtagcaga aatccttctt gaaggtttac
    17581 gtcgtctgga ataccgcgga tatgactctg ccggtctggc cgttgttgat gcggaaggtc
    17641 atatgactcg cctgcgtcgc ctcggtaaag tccagatgct ggctcaggca gcggaagaac
    17701 atcctctgca tggcggcacc ggtattgctc acactcgctg ggcgacccac ggtgaacctt
    17761 cagaagctaa cgcgcatccg catgtttctg aacacattgt ggtggtgcat aatggcatca
    17821 tcgaaaacca tgaaccgctg cgtgaagagt taaaagcgcg tggctatacc ttcgtttctg
    17881 aaaccgacac cgaagtgatt gcccatctgg tgaactggga gctgaaacaa ggcgggactc
    17941 tgcgtgaggc tgttctgcgt gctatcccgc agctgcgcgg tgcgtacggt acagtgatca
    18001 tggactcccg tcacccggat accctgctgg cggcacgttc tggtagtccg ctggtgattg
    18061 gcctgggtat gggcgaaaac tttatcgctt ctgaccagct ggcgctgttg ccggtgaccc
    18121 gtcgctttat cttccttgaa gagggcgata ttgcggaaat cactcgccgt tcggtaaaca
    18181 tcttcgataa aactggcgcg gaagtaaaac gtcaggatat cgaatccaat ctgcaatatg
    18241 acgcgggcga taaaggtatt taccgtcact acatgcagaa agagatctac gaacagccga
    18301 acgcgataaa aaacaccctt accgggcgca tcagccacgg tcaggttgat ttaagcgagc
    18361 tgggaccgaa cgccgacgaa ctgctgtcga aggttgagca tattcagatc ctcgcctgtg
    18421 gtacttctta taactccggt atggtttccc gctactggtt tgaatcgctg gcaggtattc
    18481 cgtgcgacgt cgaaatcgcc tctgaattcc gctatcgtaa atctgccgtg cgtcgtaaca
    18541 gcctgatgat caccttgtca cagtctggcg aaaccgcgga taccctggcg ggcctgcgtc
    18601 tgtcgaaaga gctgggttac cttggttcgc tggcaatctg taacgttccg ggttcttctc
    18661 tggtgcgcga atccgatctg gcgctgatga ccaacgcggg tacggaaatc ggcgtggcgt
    18721 ccactaaagc attcaccact cagttaactg tgctgttgat gctggtggcg aagctgtctc
    18781 gcctgaaagg tctggatgcc tccattgaac atgacattgt gcatggtctg caggcgctgc
    18841 cgagccgtat tgagcagatg ctgtctcagg ataaacgcat tgaagctctg gcagaagatt
    18901 tctctgacaa acatcacgcg ctgttcctgg gccgtggcga tcagtaccca atcgcgctgg
    18961 aaggcgcatt gaagctgaaa gagatctctt acattcacgc tgaagcctac gctgcaggcg
    19021 agctgaaaca cggcccgctg gcgctgattg acgcggatat gccggttatc gtcgttgcac
    19081 cgaacaacga actgttggaa aaactgaaat ccaacattga agaagttcgc gcgcgtggcg
    19141 gtcaattgta tgtcttcgcc gatcaggatg cgggttttgt aagtagcgat aacatgcaca
    19201 tcatcgagat gccgcatgtg gaagaggtga ttgcaccgat cttctacacc gttccgctgc
    19261 agctgctggc ttaccatgtc gcgctgatca aaggcaccga cgttgaccag ccgcgtaacc
    19321 tggcaaaatc ggttacggtt gagtaatcac tatcatagat aaaaaagggc ttgtgggcgg
    19381 acaataaagt cttaaactga acaaaataga tctaaactat gacaataaag tcttaaacta
    19441 gacagaatag ttgtaaactg aaatcagtcc agttatgctg tgaaaaagca tactggactt
    19501 ttgttatggc taaagcaaac tcttcatttt ctgaagtgca aattgcccgt cgtattaaag
    19561 aggggcgtgg ccaagggcat ggtaaagact atattccatg gctaacagta caagaagttc
    19621 cttcttcagg tcgttcccac cgtatttatt ctcataagac gggacgagtc catcatttgc
    19681 tatctgactt agagcttgct gtttttctca gtcttgagtg ggagagcagc gtgctagata
    19741 tacgcgagca gttcccctta ttacctagtg ataccaggca gattgcaata gatagtggta
    19801 ttaagcatcc tgttattcgt ggtgtagatc aggttatgtc tactgatttt ttagtggact
    19861 gcaaagatgg tccttttgag cagtttgcta ttcaagtcaa acctgcagca gccttacaag
    19921 acgagcgtac cttagaaaaa ctagaactag agcgtcgcta ttggcagcaa aagcaaattc
    19981 cttggttcat ttttactgat aaagaaataa atcccgtagt aaaagaaaat attgaatggc
    20041 tttattcagt gaaaacagaa gaagtttctg cggagctttt agcacaacta tccccattgg
    20101 cccatatcct gcaagaaaaa ggagatgaaa acattatcaa tgtctgtaag caggttgata
    20161 ttgcttatga tttggagtta ggcaaaacat tgagtgagat acgagcctta accgcaaatg
    20221 gttttattaa gttcaatatt tataagtctt tcagggcaaa taagtgtgca gatctctgta
    20281 ttagccaagt agtgaatatg gaggagttgc gctatgtggc aaattaatga ggttgtgcta
    20341 tttgataatg atccgtatcg cattttggct atagaggatg gccaagttgt ctggatgcaa
    20401 ataagcgctg ataaaggagt tccacaagct agggctgagt tgttgctaat gcagtattta
    20461 gatgaaggcc gcttagttag aactgatgac ccttatgtac atcttgattt agaagagccg
    20521 tctgtagatt ctgtcagctt ccagaagcgc gaggaggatt atcgaaaaat tcttcctatt
    20581 attaatagta aggatcgttt cgaccctaaa gtcagaagcg aactcgttga gcatgtggtc
    20641 caagaacata aggttactaa ggctacagtt tataagttgt tacgccgtta ctggcagcgt
    20701 ggtcaaacgc ctaatgcatt aattcctgac tacaaaaaca gcggtgcacc aggggaaaga
    20761 cgttcagcga caggaacagc aaagattggc cgagccagag aatatggtaa gggtgaagga
    20821 accaaggtaa cgcccgagat tgaacgcctt tttaggttga ccatagaaaa gcacctgtta
    20881 aatcaaaaag gtacaaagac caccgttgcc tatagacgat ttgtggactt gtttgctcag
    20941 tattttcctc gcattcccca agaggattac ccaacactac gtcagtttcg ttatttttat
    21001 gatcgagaat accctaaagc tcagcgctta aagtctagag ttaaagcagg ggtatataaa
    21061 aaagacgtac gacccttaag tagtacagcc acttctcagg cgttaggccc tgggagtcgt
    21121 tatgagattg atgccacgat tgctgatatt tatttagtgg atcatcatga tcgccaaaaa
    21181 atcataggaa gaccaacgct ttacattgtg attgatgtgt ttagtcggat gatcacgggc
    21241 ttttatatcg gctttgaaaa tccgtcttat gtggtggcga tgcaggcttt tgtaaatgct
    21301 tgctctgaca aaacggccat ttgtgcccag catgatattg agattagtag ctcagactgg
    21361 ccgtgtgtag gtttgccaga tgtgttgcta gcggaccgtg gcgaattaat gagtcatcag
    21421 gtcgaagcct tagtttctag ttttaatgtg cgagtggaaa gtgctccacc tagacgtggc
    21481 gatgctaaag gcatagtgga aagcactttt agaacactac aagccgagtt taagtccttt
    21541 gcacctggca ttgtagaggg cagtcggatc aaaagccatg gtgaaacaga ctataggtta
    21601 gatgcatctc tgtcggtatt tgagttcaca caaattattt tgcgtacgat cttattcaga
    21661 aataaccatc tggtgatgga taaatacgat cgagatgctg attttcctac agatttaccg
    21721 tctattcctg tccagctatg gcaatggggt atgcagcatc gtacaggtag tttaagggct
    21781 gtggagcaag agcagttgcg agtagcgtta ctgcctcgcc gaaaggtctc tatttcttca
    21841 tttggcgtta atttgtgggg tttgtattac tcggggtcag agattctgcg tgagggttgg
    21901 ttgcagcgga gcactgatat agctagacct caacatttag aagcggctta tgacccagtg
    21961 ctggttgata cgatttattt gtttccgcaa gttggcagcc gtgtattttg gcgctgtaat
    22021 ctgacggaac gtagtcggca gtttaaaggt ctctcatttt gggaggtttg ggatatacaa
    22081 gcacaagaaa aacacaataa agccaatgcg aagcaggatg agttaactaa acgcagggag
    22141 cttgaggcgt ttattcagca aaccattcag aaagcgaata agttaacgcc cagtactact
    22201 gagcccaaat caacacgcat taagcagatt aaaactaata aaaaagaagc cgtgacctcg
    22261 gagcgtaaaa aacgtgcgga gcatttgaag ccaagctctt caggtgatga ggctaaagtt
    22321 attcctttca acgcagtgga agcggatgat caagaagatt acagcctacc cacatacgtg
    22381 cctgaattat ttcaggatcc accagaaaag gatgagtcat gagtgctacc cggattcaag
    22441 cagtttatcg tgatacgggg gtagaggctt atcgtgataa tccttttatc gaggccttac
    22501 caccattaca agagtcagtg aatagtgctg catcactgaa atcctcttta cagcttactt
    22561 cctctgactt gcaaaagtcc cgtgttatca gagctcatac catttgtcgt attccagatg
    22621 actattttca gccattaggt acgcatttgc tactaagtga gcgtatttcg gtcatgattc
    22681 gaggtggcta cgtaggcaga aatcctaaaa caggagattt acaaaagcat ttacaaaatg
    22741 gttatgagcg tgttcaaacg ggagagttgg agacatttcg ctttgaggag gcacgatcta
    22801 cggcacaaag cttattgtta attggttgtt ctggtagtgg gaagacgacc tctcttcatc
    22861 gtattctagc cacgtatcct caggtgattt accatcgtga actcaatgta gagcaggtgg
    22921 tgtatttgaa aatagactgc tcgcataatg gttcgctaaa agaaatctgc ttgaattttt
    22981 tcagagcgtt ggatcgagcc ttgggctcga actatgagcg tcgttatggc ttaaaacgtc
    23041 atggtataga aaccatgttg gctttgatgt cgcaaatagc caatgcacat gctttagggt
    23101 tgttggttat tgatgaaatt cagcatttaa gccgctctcg ttcgggtgga tctcaagaga
    23161 tgctgaactt ttttgtgacg atggtgaata ttattggcgt accagtgatg ttgattggta
    23221 cccctaaagc acgagagatt tttgaggctg atttgcggtc tgcacgtaga ggggcagggt
    23281 ttggagctat attctgggat cctatacaac aaacgcaacg tggaaagccc aatcaagagt
    23341 ggatcgcttt tacggataat ctttggcaat tacagctttt acaacgcaaa gatgcgctgt
    23401 tatcggatga ggtccgtgat gtgtggtatg agctaagcca aggagtgatg gacattgtag
    23461 taaaactttt tgtactcgct cagctccgtg cgctagcttt aggcaatgag cgtattaccg
    23521 ctggtttatt gcggcaagtg tatcaagatg agttaaagcc tgtgcacccc atgctagagg
    23581 cattacgctc gggtatccca gaacgcattg ctcgttattc tgatctagtc gttcccgaga
    23641 ttgataaacg gttaatccaa cttcagctag atatcgcagc gatacaagaa caaacaccag
    23701 aagaaaaagc ccttcaagag ttagataccg aagatcagcg tcatttatat ctgatgctga
    23761 aagaggatta cgattcaagc ctgttaattc ccactattaa aaaagcgttt a
//
LOCUS       DAJTQY010000060        22159 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.060, whole genome shotgun sequence.
ACCESSION   DAJTQY010000060 DAJTQY010000000
VERSION     DAJTQY010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22159
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.060"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(<1..408)
                     /gene="sxy"
                     /locus_tag="OO349_002473"
     CDS             complement(<1..408)
                     /gene="sxy"
                     /locus_tag="OO349_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HCR5867507.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEV"
     gene            627..1136
                     /gene="sulA"
                     /locus_tag="OO349_002474"
     CDS             627..1136
                     /gene="sulA"
                     /locus_tag="OO349_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HCR5867508.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQAFGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLTDDLTEEEHAELVDAANEGNAMGFIMRPVSASSHTTRQLSG
                     LKIHSNLYH"
     gene            1492..2538
                     /gene="ompA"
                     /locus_tag="OO349_002475"
     CDS             1492..2538
                     /gene="ompA"
                     /locus_tag="OO349_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HCR5867509.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKANVPGGASFKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDANTIGTRPDNGLLSLGVSYRFGQGEAAPVVAPAPAPEVQTKHFT
                     LKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLS
                     ERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEI
                     EVKGIKDVVTQPQA"
     gene            complement(2614..3066)
                     /gene="matP"
                     /locus_tag="OO349_002476"
     CDS             complement(2614..3066)
                     /gene="matP"
                     /locus_tag="OO349_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HCR5867510.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            3251..5011
                     /locus_tag="OO349_002477"
     CDS             3251..5011
                     /locus_tag="OO349_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HCR5867511.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARTLQSDAGQLVGDHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRVQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            5080..5598
                     /gene="fabA"
                     /locus_tag="OO349_002478"
     CDS             5080..5598
                     /gene="fabA"
                     /locus_tag="OO349_002478"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HCR5867512.1"
                     /translation="MVDKRESYTKEDLLASGHGELFGAKGPQLPAPNMLMIDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDCRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(5668..5835)
                     /gene="rmf"
                     /locus_tag="OO349_002479"
     CDS             complement(5668..5835)
                     /gene="rmf"
                     /locus_tag="OO349_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HCR5867513.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLSQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(6091..6654)
                     /gene="pqiC"
                     /locus_tag="OO349_002480"
     CDS             complement(6091..6654)
                     /gene="pqiC"
                     /locus_tag="OO349_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HCR5867514.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            complement(6651..8291)
                     /gene="pqiB"
                     /locus_tag="OO349_002481"
     CDS             complement(6651..8291)
                     /gene="pqiB"
                     /locus_tag="OO349_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HCR5867515.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            complement(8296..9549)
                     /gene="pqiA"
                     /locus_tag="OO349_002482"
     CDS             complement(8296..9549)
                     /gene="pqiA"
                     /locus_tag="OO349_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HCR5867516.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            complement(9679..11586)
                     /locus_tag="OO349_002483"
     CDS             complement(9679..11586)
                     /locus_tag="OO349_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5867517.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLQEKEEALRVEELQNAEFDLKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVNGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRAFSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKSEEAAAPKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            complement(11598..13706)
                     /gene="rlmKL"
                     /locus_tag="OO349_002484"
     CDS             complement(11598..13706)
                     /gene="rlmKL"
                     /locus_tag="OO349_002484"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HCR5867518.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQSGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERHKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTRRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            13950..15059
                     /gene="ycbX"
                     /locus_tag="OO349_002485"
     CDS             13950..15059
                     /gene="ycbX"
                     /locus_tag="OO349_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HCR5867519.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRTNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLTR"
     gene            complement(15056..15598)
                     /gene="zapC"
                     /locus_tag="OO349_002486"
     CDS             complement(15056..15598)
                     /gene="zapC"
                     /locus_tag="OO349_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HCR5867520.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKDQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(15772..16782)
                     /gene="pyrD"
                     /locus_tag="OO349_002487"
     CDS             complement(15772..16782)
                     /gene="pyrD"
                     /locus_tag="OO349_002487"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HCR5867521.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEDELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            complement(16893..17632)
                     /locus_tag="OO349_002488"
                     /pseudo
     CDS             complement(16893..17632)
                     /locus_tag="OO349_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
     gene            complement(17598..18113)
                     /locus_tag="OO349_002489"
     CDS             complement(17598..18113)
                     /locus_tag="OO349_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5867522.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            complement(18121..18663)
                     /locus_tag="OO349_002490"
     CDS             complement(18121..18663)
                     /locus_tag="OO349_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5867523.1"
                     /translation="MKKKTIYQCVILFFSLLNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGYFNISDFQAANTVSTAADLNIDITGCAAGITGADVLFSGEADTLAP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            complement(18761..19744)
                     /locus_tag="OO349_002491"
     CDS             complement(18761..19744)
                     /locus_tag="OO349_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5867524.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYIGQCHTTQGNPYI
                     GVNFGVKTLDEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGLRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATSDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIRE"
     gene            complement(19735..22107)
                     /locus_tag="OO349_002492"
                     /pseudo
     CDS             complement(19735..22107)
                     /locus_tag="OO349_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
BASE COUNT         5493 a         5802 c         5205 g         5659 t
ORIGIN      
        1 cacctccccg agaatcgctt ccagatgaaa agacatattg ggcaaatctt tcagtctttc
       61 ccgggtattg cgcgtgcttt tctctttcag cgctgcatcg agagaatatt tcgacagacg
      121 caccagcttc agttgatttc gccatagact ttcatcaacc cgatagtaat tgagggtaac
      181 ggatcggcca cactttttat atgtcagcca gacaggcgga tgttttacac agtactgtgc
      241 actttgctca caagcccgaa gatacaactc accatcagaa accatcgcaa acaccgtgtc
      301 gtcaacggtc aggctgtaac tgccaaacaa tgatcggtat tcaattgtgc ccaacgttgc
      361 caggtattct tgtgatttat agatccgctt ataggagagg cttttcataa aattcctttt
      421 aaaatcataa cataaaagaa tgattcacat taacggatcc gttaactacg aaaataggca
      481 acttattctt aaggggcaag attaatttat gttttcccgt caccaacgac aaaatttgcg
      541 aggctctttc cgaaaatagg gttgatcttt gttgtcactg gatgtactgc acatccatac
      601 agtaactcac aggggctgga ttgattatgt acacttcagg ctatgcacat cgttcttcgt
      661 cgttctcatc cgcagcaagt aaaattgcgc gtgtctctac ggaaaacact acagccgggc
      721 ttatcagtga agttgtctat cgcgaagatc agcccatgat gacgcaactt ctactgttgc
      781 cattgttaca gcaactcggt cagcaatcgc gctggcaact ctggttaaca ccgcaacaaa
      841 aactgagtcg ggaatgggtt caggcatttg ggctaccctt aacgaaagta atgcagatta
      901 gccagctctc cccttgccac acagtggagt caatggttcg cgctttacgc acgggcaatt
      961 acagtgtggt gatcggttgg ttgacagatg atttgactga agaagagcat gctgaacttg
     1021 ttgatgcggc aaatgaaggt aacgctatgg ggtttattat gcgtccggta agcgcatcct
     1081 ctcacacaac gagacaactt tccgggctaa aaattcactc taatttgtat cattaagtaa
     1141 atttaggatt aatcctagaa ctttttttgt cgcccagcca atgctttcag tcgtgactaa
     1201 ttttccttgc gaaggcttgt ctgaagcggt ttccgcgttt tttttctgta aattgtcgct
     1261 gacaaaaaga ttaaacatac cttatacaag actttttttt catatgcctg acggagttca
     1321 cacttgtaag ttttcaacta cgttgtagac tttacatcgc caggggtgct cggcataagc
     1381 cgaagatatc ggtagagtta atattgagca gatcccccgg tgaaggattt aaccgtgtta
     1441 tctcgttgga gatattcatg gcgtattttg gatgataacg aggcgcaaaa aatgaaaaag
     1501 acagctatcg cgattgcagt ggcactggct ggtttcgcta ccgtagcgca ggccgctccg
     1561 aaagataaca cctggtacac tggtgctaaa ctgggctggt cccagtacca tgacactggt
     1621 tttattccta acaatggtcc gacccacgaa aaccaactgg gtgcaggtgc ttttggtggt
     1681 taccaggtta acccgtatgt tggttttgaa atgggttacg actggttagg tcgtatgccg
     1741 tacaaaggcg acaacatcaa cggcgcatac aaagctcagg gcgttcagct gaccgctaaa
     1801 ctgggttacc caatcactga cgatctggac atctacactc gtctgggtgg tatggtatgg
     1861 cgtgcagaca ccaaggctaa cgtacctggt ggcgcatcct ttaaagacca cgacactggc
     1921 gtttctccgg ttttcgcagg tggtgttgag tacgcgatca ctcctgaaat cgctacccgt
     1981 ctggaatacc agtggaccaa caacatcggt gacgcaaaca ccatcggtac tcgtccggac
     2041 aacggtctgc tgagcctggg tgtttcctac cgtttcggtc agggcgaagc agctccggta
     2101 gttgctccag ctccggcacc ggaagtacag accaagcact tcactctgaa gtctgacgtt
     2161 ctgttcaact tcaacaaagc aaccctgaaa ccggaaggtc aggctgctct ggatcagctg
     2221 tacagccagc tgagcaacct agatccgaaa gacggttccg tagttgttct gggttacact
     2281 gaccgcatcg gttctgacgc ttacaaccag ggtctgtccg agcgccgtgc tcagtctgtt
     2341 gttgattacc tgatctccaa aggtatcccg gcagacaaga tctccgcacg tggtatgggc
     2401 gaatccaacc cggttactgg caacacctgt gacaacgtga aacagcgtgc tgcactgatc
     2461 gactgcttgg ctccggatcg tcgcgtagag atcgaagtta aaggtatcaa agacgttgta
     2521 actcagccgc aggcttaagt tatcgtctgg tagaaaaacc ccgctgctgc ggggtttttt
     2581 ttgcctttag taaattgaac tgactttcgt cagttattcc ttacccagca atgcctgcag
     2641 atcctgctta agagaagaca ttttattcgc gtatttctct ttgttttccg catcttcaat
     2701 cagctgaaca atcgtttcag aaagcgtttt accgcgacgc tgtgcaagac cagccagacg
     2761 ttgccagaca ataaattcca gatcgatcga ttttttgcgc gtatgctggt gttccgcatt
     2821 aaaatggcgc ttccgtctgg cccgaatggt ctgcttcatg cgattgacca gttccggatt
     2881 catatgcttg tcaatccagc catttaccag cacgggttca ttttcgagcg agagcaacac
     2941 atcgacggct tcctgggcgg cactggcttc tatgtaacgg gtaattaact ccccttcgcg
     3001 atgcttcttc accagatact tccatttcca accgctttca agattttcaa gttgttgata
     3061 tttcatagcg atctcaatgt taccgtgtaa ctctttacag aatatcagct ttttaggcca
     3121 gtgaagaaaa gaatctccat cctgtgagct ttaacgccca gccaggagta ttgtcggtgc
     3181 tttctacgtg tgctgtagtg ccggttacgg tataatcgcg gctttgacaa cagactaaaa
     3241 aacatcaact ttgaccatta cgaaacttgc atggcgtgac ctggttcctg ataccgatag
     3301 ctatcaggaa atatttgctc agccacattt gattgacgaa aacgatcctt tattcagtga
     3361 tactcaaccg cgactgcaat ttgcgctgga gcagttgctg catacgcgag catcctcctc
     3421 ttttatgctg gctaaggccc cggaagagtc tgagtatctg aatcttattg ccgatgccgc
     3481 gcgtacgcta caaagcgatg caggccaact ggtgggcgat cactatgagg tttccggcca
     3541 ctccatccgc ttacgtcacg cagtgagtgc agatgataat tttgcgactt taacgcaagt
     3601 tgtcgctgcc gactgggtag aagcggagca actctttggc tgcctgcgcc agtttaatgg
     3661 cgacattacc ctgcagcctg gtctggtgca tcaggcaaat ggcggtattc tcatcatctc
     3721 tttgcgtaca ctgctggcgc aacctctgct gtggatgcgg ctgaaaaata tcgttaaccg
     3781 cgagcgtttt gactgggttg cgtttgatga gtcgcgccct ctccccgtct ctgtgccttc
     3841 gatgccattg aagctgaaag tcattctggt aggcgaacgc gaatcattgg ctgatttcca
     3901 ggagatggag ccagagcttt cagagcaggc tatttatagc gaatttgaag atactctgca
     3961 gattgtcgat gcggagtcag taagccagtg gtgtcgctgg gtgacattta ccgccagaca
     4021 taatcactta cctgccccgg gagcggatgc ttggccggta cttatccgcg aagcagcacg
     4081 ctacaccggt gaacaagaaa cacttccgct tagcccgcag tggatcctcc gccagtgtaa
     4141 agaggtcgcc tccctgtgtg atggcgacac cttctccggc gagcagctaa acttaatgct
     4201 gcagcagcgt gaatggcgcg aaggtttcct cgctgaacgt atgcaggatg agatccttca
     4261 ggagcaaatc ctgattgaaa ccgaaggcga acgcatcggg caaattaacg ccctttcggt
     4321 cattgaattt ccgggtcatc cacgcgcttt tggcgaacct tctcgcatta gctgcgttgt
     4381 gcatattggc gatggtgaat tcaccgacat cgaacgcaaa gcggagcttg gcggcaatat
     4441 ccatgcgaaa gggatgatga tcatgcaggc gttcctgatg tcggaactac agcttgagca
     4501 acagatcccc ttctcagcat cgctgacatt tgagcagtca tacagtgaag tggatggcga
     4561 tagtgcctcg atggctgaac tctgcgccct gataagcgcc ctcgctgatg tgccagtgaa
     4621 tcagagtatc gctatcacag gttcagtcga tcagttcggc cgcgtccagc cggtcggtgg
     4681 tttaaatgag aaaatcgaag gcttctttgc tatttgccag caacgtgagt taacggggaa
     4741 acaaggtgtc attatcccca ctgctaacgt tcgccattta agtcttcaca gtgaactggt
     4801 gaaagcggta gaagaaggca aattcaccat ctgggcagta gacgatgtga ctgacgcact
     4861 gccgttatta ttaaatctgg tgtgggatgg cgaaggccaa acgacgctga tgcaaaccat
     4921 ccaggaacgt atcgcgcaag catcgcaaca ggaaggacgt caccgttttc catggccatt
     4981 acgttggctg aactggttta ttccgaactg atcggacttg ttcagcgtac acgtgttagc
     5041 tatcctgcgt gcttcaataa aataaggctt acagagaaca tggtagataa acgcgaatcc
     5101 tatacaaaag aagaccttct tgcctctggt cacggtgaac tgtttggcgc taaaggcccg
     5161 caattgccag caccgaacat gctgatgata gaccgtgtgg tcaaaatgac cgaaacgggt
     5221 ggtaacttcg acaaagggta tgttgaagca gaactggata tcaatccgga tctgtggttc
     5281 ttcggatgcc acttcattgg cgatccggtt atgccgggat gcctgggcct ggacgcaatg
     5341 tggcagctgg tagggttcta cctcggctgg ctgggcggcg aaggtaaagg ccgcgcgctg
     5401 ggcgttggcg aagtgaaatt cactggtcag gtactgccga cagcgaaaaa agtgacctac
     5461 cgtattcact ttaaacgcat tgttaatcgt cgtctgatta tgggtctggc agatggcgaa
     5521 gtgctggttg attgtcgtct gatctatacc gccagcgacc tgaaagtcgg tctgttccag
     5581 gatacgtctg ccttctgatt atcatcagac agagtatcaa aaggcgaaac ctccgcaatg
     5641 cggaggtttc tttttaaaga gacagaatca ggccattact accctgtccg ccatggcttc
     5701 tcgccagcct cccagccatt gtgacctttg actcagcgtc tgatagggac acatttcttt
     5761 tgagcgtccg gcgatgccgg cctgataacc acgttgatgt gcccgttcca ggcgatctcg
     5821 tttttgtctc ttcatgcctc gtttccctca tactggtttc tggtggaaaa gaaaacagtg
     5881 attactaaat gtgcaatcac agttaagcaa taccgcgaat cataactgcc gtcaatgcgc
     5941 aaaattcacg ccactgtcat atttgtgagc tacacgggca tgtcggttgt acaaaaaatg
     6001 tgaagaggaa caaatttttc cgcacgttag aaacaaaaaa accgcctcag gcaacatacc
     6061 ggagacggtt atatttacaa atctttataa ttaaggtaga cgctttatct cttgtgcaat
     6121 agaagcggct tcctgactcc agacaccggc cagcacttta accatctcat cgtaaccatc
     6181 ctgagtttgc actccttcca gacggaacgg acgtttgatc agttgtccct ggtggttcaa
     6241 cagccactca ccactgacaa tgaccttgcc atcatagcga ccgttaaact ccgttacggt
     6301 aacattgagc gtgtcctggg cgcttcccag aggctgggag gcaaccaccc agccgggcag
     6361 ttgcgtactc aggttggcaa ccagggtgtt gcgcaactgt tgatccaacg ggctggccca
     6421 caagttgttg ttggcaatca catacttcac atcactggtt tgataaacca caccattccc
     6481 cgccagatag tcaggaacag tgacctgctc tacccataac agacgattgc cctggctggc
     6541 ggtactttgt gtaccgctct gcaccacagg taactggtaa tagtttttat taatttcgcc
     6601 ggagctgcat ccggccagcc acagtgctgc aatcgtcact agccactttt tcattgtttc
     6661 gccctcttcg gctctggatc ttttttgtcc ttcgcttcaa ataccagcgc gttactcttc
     6721 tcattgagcg ttttcagcac cggttgcagt tctcgcaaca cctgatcaag gcgctgcata
     6781 tccgccacca tcttgttgta ggctgcggag ccaggctgga agccctgcat gctgcgattc
     6841 aattcacgca acgttgactg catatccgtc ggcaactgct gcatcgactg gctggcgagg
     6901 atcttgttca tgctatccag cgtcgtttgc aggtttttca ttgtgcgctg actttcagaa
     6961 agcgtactgg ttgcctgttc aatcatcgga ttcaatggca gtttgttgat cttatccaac
     7021 gcttccatca gtcgttgctg gatttgcgcc aagccgccgc taacagtcgg gataatctga
     7081 taaccattaa attcacgaat accggttatt gcaggcgtat ttggatagaa atcgagatca
     7141 acatacagtg caccagtgac caggtttccg gttttcagcg atccgcgtaa accacgtttc
     7201 aacaattcgc caaggtgctc aacaacatcc gcattttcgc caagctgcat tttcagccgc
     7261 tctggctcga tacgaatcag taccggaata cggtaatcat cgttaaatgt ctgacgcata
     7321 ttcggcgcaa agaatggcac tttgcttacg gtacccaggc gaataccccg gaattctacc
     7381 ggagctcccg gttgcagacc gcgtaccgaa tctttaaaga acatcagata atcaatgtga
     7441 tcggtgtaca acgaatcctg aatgctcttc tgatcatcat acaaaacgaa agctgttttc
     7501 ggtgccactg gctgccctaa atccagacct tccggcacat caaagctaac accgccacta
     7561 agcagcgttg tcaatgagcc catctccaca cgcatccccg ctgacgtcag atcaaccgca
     7621 atgccactat ctttccagaa gcgaacattg ctggtcacca gtcggtcata aggtgcattg
     7681 atgaacagtt gataactgat attgcgtttt tgcgtatcga aggtgctggt ttcaaccgaa
     7741 cctacccgat agccacggaa cagcaccgga tctcctggcg agagctgccc ggcttttttg
     7801 ctatcgagaa tcacacggat acctttcgca tcaggcgggg ccaacggtgg tgagtccagc
     7861 aaatcgtatt tatccatttt gctgcctttc gcgcctggct gcagttcgat ataaactcca
     7921 gacagcagcg ttcccaggcc gctaatccct tcgcgaccaa tctgcggttt caccacccaa
     7981 aagacggtgt ctttatgcag caatttttcc ataccggaat tcagccgcgc tttgatttca
     8041 acgtgcgtca aatcatcagc cagtgtggcg ctttcaacca cgccgacgtc aacgctacgg
     8101 cttttaatgg tggttttgcc accttcaatt ccttccgcat tcgcggtgat cagggtcact
     8161 tccggtccct gatggctgta atgataaaaa agaacccagg ccccaatgag cgccgtgacg
     8221 ataggaaata tccacacggg agaccagttc ttcactttct ggattttggc ttccccatta
     8281 ttagattcca tactgtcagg actcctcatg ctctgattca ggttgacgat cccacgacaa
     8341 acgcgggtca aacgtcatag cagaaaacat tgtcattatg acgactaaag caaacattaa
     8401 tgcacccatt gccggataaa tactcattaa acctcccata cgcaccagcg ccgagagcac
     8461 cgcgataacg aaaacgtcaa tcatcgacca gcggcctaca aactcaacaa cttcataaat
     8521 caaatgcatt ctttcactgt cgcgcttgcc atgccctttg gcatcccaac acagccacgc
     8581 gatggcgatc atctttaacg ttggcaccat aatactggcc agaaagatca ccgcagcgac
     8641 gggataagag ccttcgctcc ataacagaat gaccccagcg agaatcgtcg acggcatctt
     8701 cgagcctaat aaatccgtca ccatgatggg caaaatatta gccggaagat acagcatgat
     8761 ggacgttaca agcagcgcga gtgtccactg caggctgttt ctacgccgaa cgtacccttt
     8821 ggtactacaa cgcgggcaca cgggttcatc agcaggaagg attgccgtac aacaggagca
     8881 cgaacgcagc ccctgacgta tccccgtgac gcctggtttt agcggctggc gcagttctgg
     8941 catcggggcg atgtcgtccc ataaccagcg acgatcaacg cactgaaaag cgcgcagttg
     9001 caggacacaa aataaacacc aggggagaaa gctgctgcct accccaatgc tgccgtaagc
     9061 catcagttta acgaaactga ccagcacacc cgcgaggaaa atctccgcca ttccccaggt
     9121 tttgagttga aaaagcaccc gtgccagttg ctcttttaaa cggaccggta attccgcgcg
     9181 attcaccagt aacagaatgg ttatcagaca aaacgcggga accagttgca caaacaacag
     9241 gaaaaaggtg ccgaggctgg cgtagtcctc agaaaaaagc acgccgggaa tttccagtaa
     9301 tgtaatttca ctggtaactc ctgcaacgtt catattcaca aaaggaaaca agttggacag
     9361 caacagcatg aacagtgcag ccaacgcata ggcggtggga cgctgccgag gggcatccca
     9421 cgccacggtt aacgttgtgc cacaccgggg acatgccgct ttctgaccat gctcaaggcg
     9481 cggtaacgcc accagcatgt cacactgcga gcacaggatg tgcttcgcgg catgatgatg
     9541 ttcgcacatt ggtactcctt aatgatgcgc tattttacag atgactaaca aagtgttggt
     9601 tttcatgcct gataaaccca gttcatcagg caattttacc gttgtcagct gtctctgttt
     9661 aaatcgacta ttttgcgatc agccaccatt ttttaacgct tcaagatact cccagcgttc
     9721 aaaggcttgc tccagctcct gctctgcagc agccatatca gcaagcactt tttgcgtctg
     9781 ctcatgcggc tgactgaaga aggaagcatc cgccacttgc gtctgtaggg cttccagctt
     9841 cgcctccaga tcttcgagca attgcggtag ctgctccagt tcgcgctgca atttatagct
     9901 tagtttgcta ctgctgcgtt ttacagtttc tgcttttggc gcggcggctt cttcgctttt
     9961 tttcaccgca ggctgtttga gtgccacata ttgttcttgc tgaccacggg catcatgata
    10021 accgccaaca taacgaccaa ttttaccgcc gccttcgaag atccagcatt ctgtaacggt
    10081 gttatcgaca aactgacgat cgtggctaac cagcaatacc gtgccctgat agctgtcgat
    10141 cagttcttcc agcagttcca gcgtttcgac atcaagatcg ttggtcggtt cgtcaagaat
    10201 caataagttg cttggtttga ggaacaaacg cgccagcagc aagcggttcc gctcaccgcc
    10261 agaaaacgca cgtactggcg tcatcgcccg tttcggatgg aacagaaagt cctgcaaata
    10321 gcccaatacg tggcgtggct tgccattaac catcacctct tgcttacctt cggcaaggtt
    10381 atccatcacc gttttatcgg gatccagttc cgcgcggtgc tgatcgaaat aagccacttc
    10441 cagtttggtg ccaacgtgaa tacgcccgct gtccgcttga agctgaccga gcatcagttt
    10501 cagcagcgtg gttttgccgc acccattcgg accaatcagg gcaattttgt cgccacgtag
    10561 aacctgggca gaaaaatctt tcaccagttg cttaccgtta acctggtagc aaacgtcttc
    10621 catttcgaaa acgatcttac cggagcggct ggcctcttcc acctgcatct ttgcggtacc
    10681 catcacttcg cgacgttcac cacgttcgcg acgcatcgct ttcagggcgc gtacgcggcc
    10741 ttcattacgg gtacggcgtg ctttgatccc ctggcgaatc cacacttctt cctgcgccag
    10801 tttgagatcg aactcggcat tttgtagttc ttccacgcgc agggcttctt ctttttcctg
    10861 caggtactgg tcgtaattcc ctggataggt caccagcttg ccgcgatcga gatcaacaat
    10921 gcgcgtcgcc atattgcgga taaacgaacg gtcgtgggag atgaaaataa tcgtcccatt
    10981 gaaggtttta aggaacccct ccagccagtc gatggtttcg atatccagat ggttagtcgg
    11041 ttcgtccagc agcaatacgc gcggattgct caccagcgcg cgtcctaatg ccgctttacg
    11101 caaccagccg ccggaaagcg acgacagcgc aacgttagga tctaacccca gttgcgccag
    11161 cacttcgttg atgcggtttt ccagctgcca caggttgtgg tgatccagct gttcctgaac
    11221 cttcgccagt tcgttgagat ttttctcgct cgggtcgttc atcaccaggc gcgaaatatc
    11281 gtgatagcgt ttcagatatt ccgcttgttc ttcaatgcct tcggcaacga aatcataaac
    11341 gctaccctca acgttacgcg gtggatcctg ttgcagacga gctacaatca aatcttgctc
    11401 ataaataatg cgaccgtcat ccagcccttg ttcacggttg aggattttca ttaacgtcga
    11461 ttttcctgcg ccgttgcggc ccaccagaca aacacgttcg ttatcttcga tatgcagttc
    11521 tgcgttatcg agaagcggcg cgtcgctgaa cgacagccat gcgccatgca tactgattaa
    11581 tgacattact attcctttca ggctgcggta atcagccagc agttgtggat ctggcggtta
    11641 cgggcgaaat cctgggagag cgttttttgc gtaatttctt gtgctttcag tcccagtttt
    11701 gccaggccgt cgagatccat acggaagcca cgtttgttgt tcgagaacat gatcgtccca
    11761 cctgcacgca gcagacgttt caaatctttc atcagcgcca gatgatcgcg ctgaacatca
    11821 aacgcatctt ccattcgttt tgagttagag aaggttggcg gatcgataaa gatcagatcg
    11881 aactgttcat ttgcctcacg cagccacgcc aggcaatcgg cctgaatcag gcgatgcgca
    11941 cgcctagtca ggccattcag acgcaggttg cgttctgccc attccagata agtacgcgac
    12001 atatccacgg tggtggtgct gcgtgcaccg cctaatcccg catgcacggt ggcgctgccg
    12061 gtataagaaa acaggttgag gaaatctttg cctttgctca tctgaccgag catacggcgg
    12121 gcgatacggt gatcgaggaa cagaccagta tcgagataat ccgtcaggtt cacccacaag
    12181 tgagcgttat attcggtaac ttcaagaaac tcgcccttct cgcccagttt ctggtactgg
    12241 tttttgccct tatggcgttc acgggttttc agcaccagtt tgtttggcgc aatccccagt
    12301 accgaaatgg ttgcagcgat aatatcgaac agacgctgac gcgctttgtg cgcatcaata
    12361 gtttttggcg gcgcatactc ctgcaccacc acccagtcgg cataacggtc aacggcaacg
    12421 ttatattccg gcagatcggc gtcatacagg cggtaacatt caatcccttc ctggcgtgcc
    12481 cacttctcga attttttgag gttcttacgc aggcggttgg cgtaatcttc cgctaccatt
    12541 gccggtttac tgtctggtgt gctttcggca acgtggtaat ttttctgtac gcagtccagc
    12601 gggccgtttt tcgccttgta ctgtttgtct gcacgcagct gcaggcagct tagcagatcc
    12661 ggcgaggcac taaacaaaga gagattccag ccaccgaact ggtttttcat gattcggccc
    12721 agcagactat gcagcgcaat cagcgccggt tcgctgtcca gacgttcacc gtatggcggg
    12781 ttgctcaaca ctgtaccgta cggccctttc ggcagcggat tggtcagttg cgcgacatct
    12841 ttcacctcaa aggtgatcag ttcaccaatc cccgcaagac gggcgttagt gcgcgcacgt
    12901 tgaatcaccc gtgcgtcgct gtccgaaccg taaaaatgag agctatactc agccaggcct
    12961 ttacgggcgc gagtttgcgc ttccgctttc acttcctgcc agatagcttc atcatgctgc
    13021 gcccagccgc taaagcccca acgcccacgg tgcaagcctg gtgcgcgatc ggtcgccagc
    13081 atcgccgctt caatcagcaa cgtaccggaa ccacacatcg gatcgagcag cggtgttcct
    13141 gactgccagc cggatcgcat cacaatagcg gctgccaggg tttctttgat cggcgcaata
    13201 ccagcacgat cgcgatagcc acgcagatgt aaaccatcac cactcagatc aagagcgata
    13261 ctggcggttt ctttatgcag ccagacgtta acgcggatat ccggcgcatc gcgatcaaca
    13321 tttggacgcg gcagattttt ccgcgtgaaa gcatcgacga tcgcgtcttt cactttcatc
    13381 gcgccgtact gactgttgcg gatggtgtca ttcaaaccac tgaagtggac agcgaaggtc
    13441 gcgccaggat taaacatctc tgtccagttg atcgcctgaa caccgagata gaggtctaaa
    13501 tcgctgtaaa ccttacactc gcccagcggc aacataatac gcgaggccag gcggctccac
    13561 atcaggctct ggtaaacaag ccgtgtgtcg cccttgaaat ggaccccacc ctgaaccacc
    13621 tggcattcaa cggcccccag attttccagt tcagttttta acagctcttc cagcccacgg
    13681 gccgtactgg caaacagaga attcatatcg tcacttttac tctaagagcc taccgcagta
    13741 ggcgtaattg ttgcagtcag tctggacatg gagagcgcgg agaaaccgga gcgtacacaa
    13801 gtacgtgagg atttcgagca caacccaggg ccaaactgac aaataaaata gcctaacggg
    13861 acaggcgcta agaaaattgt tgcgcattat agctaataag cgcgtcatgt cataaagttg
    13921 agggcttatt ttcatttgag gaccgcaccg tggcgacatt aacccggctt tttattcatc
    13981 ctgttaaatc gatgcgcggc attggtctta cacatgctct ggctgatgtc agtggtctgg
    14041 ccttcgatcg catctttatg atcacggaac ctgacggtac gtttattacc gctcgccagt
    14101 ttccccagat ggtacggttt actccttcac ctgtgcatga tggcttgcat ctcaccgcac
    14161 cagatggcag tagcgcatat gttcgttttg ctgatttcgc cacacaagac gcaccaaccg
    14221 aagtttgggg cacacatttt accgcgcgaa ttgcgccaga cgcgatcaac aaatggctaa
    14281 gtggattttt ctcccgcgaa gtgcaattac gctgggtggg gccacaaatg acccggcgcg
    14341 tgaaacgcca caacactgta cctctgtcat ttgctgatgg ctatccttac cttcttgcta
    14401 acgaagcctc gttacgtgat ctccaacaac gttgtccggc cagtgtaaaa atggagcaat
    14461 tccgcaccaa tctggtggtt tccggcgcgt cagcttggga agaagatagc tggaaagtga
    14521 ttcgcattgg tgatgtggtg tttgatgtgg ttaaaccttg tagccgctgt attttcacca
    14581 ccgtcagccc agaaaaaggg caaaaacatc cggcaggcga accattaaaa acattgcaat
    14641 ctttccgcac tgcccaggat aacggcgatg tcgattttgg tcagaattta attgcccgta
    14701 atagcggcgt gattcgcgtt ggcgatgagg tggaaattct ggcaacggct ccggcaaaaa
    14761 tttacggcgc agctgccgct gatgataccg ccaacatcac gcaacaaccg gacgcaaatg
    14821 tagatattga ctggcaggga caggcatttc gtggaaataa ccaacaagtg ttgctggagc
    14881 aattagaaaa tcagggaatt cgtatccctt attcttgccg cgcgggcatt tgtggaagtt
    14941 gccgtgttca gcttttagaa ggcgaagtca cgccgctgaa aaaatcagca atgggcgatg
    15001 atggcaccat tctttgctgt agctgtgtac cgaagactgc ccttaagctg acgcgttaga
    15061 ctgcctgttc gaggctgaag ctgtcaacat tcacctgcgg tttcagcctg tcgttcatga
    15121 ttttaatggc gtcgcccaac tgcatcgcgc gacccgcaat aacaacgcag ggttgtgcca
    15181 gcaggcatag cgcggcattt tcgccagatt caacgaccag caaattgacc tgctcgtgag
    15241 tatcgctcaa ccaaacgcag gccgcatcgc ccggcatggg cacccacatt tcaccatggg
    15301 agacaaaatg ccagcttttc ggcatttgcg gtttgagata acgaattgct accagtgcat
    15361 tcagcaccag ttcggctttt tggtctttag ataaattaaa atcgcggcac ttttcttcaa
    15421 aagagaaata gagcgcggca tcatcaacac aaaagcctgc gggagaaaag gcatctggag
    15481 tcaacatttt gcgcgcaaaa cgtgagcgaa atagcatacc attggctaaa tcgagcatca
    15541 ttcgatcgtg ctcttcatcg taataccaac gccagttatc gtctggttta attcgcatct
    15601 gcctctcccc taccctataa aaccgcagca acacaatcca ttgtcgcttc gcttaataac
    15661 ctcaacaata aaagtacaaa atgtctaaat aagcaacaac gatgaatata aaacaaccag
    15721 ggctgaaaat aaagccctga ttatcttata aagaggaatt cgaagaaata attagatatg
    15781 ggtaacgatt tctttaatca gcggcggacc tttaaaaata aaaccagaat aaatttgcac
    15841 cagtgaggca cccgcagcaa tcttttcacg cgcagcgata accgagtcga tgccgccaac
    15901 accgatgatc ggtaagcgac cgtttaattc tagtgacaag cggcgaataa tttctgtgct
    15961 ttttaactga agcggacgac cacttaagcc accggtttga tcgcaatttt tcattccctg
    16021 aaccagagaa cgatcgagag tggtattggt tgcaataacg ccatcaatat tatggcgaac
    16081 taaactatcg gcaacctgga tcaattcgtc ttcagaaaga tccggcgcga tcttcactgc
    16141 gatcggcaca tatttatggt gcatcgcttg caaatcattt tgcttatttt taatcgcggt
    16201 taagagatca tccagcgctt caccatattg cagcgtgcgt aatcctgggg tattcggcga
    16261 tgaaatattg atggcgatat atcccgcata ggcatagatt ttttccatac aaatcagata
    16321 gtcatctttg ccctgctcca ctggcgtatc tttatttttg ccgatattaa tacccaagac
    16381 gccgtcataa tgggcctttt ttacgttctc tacgaggtta tcaacgccaa gattattaaa
    16441 gcccatacgg ttgatcaaac cttcggcatc taccagacga aagagacgcg gcttgtcatt
    16501 accaggctgt ggacgtggcg tgacggtacc gatctcgatc gatccaaatc ccatcgcgcc
    16561 taacgcatca atgcactccc cgtctttatc aagaccggct gccagaccaa gcggattttt
    16621 aaacgtcagg cccatgcagt taacaggttt cgcaggcact ttctgccgca ccagtgcttc
    16681 aaacggtgtt ccggtaatgc ggcgtaattg ctgaaaagta aactcatgag cgcgctctgg
    16741 atcgagctgg aaaagggctt tacgaacgaa ggggtagtac atgaactctc ctggattccc
    16801 ggtgtgcaaa ccgggggcgt attatgtgcg agttcggacc aaaagggaat tgacctgcgg
    16861 caaaaaaaaa gcaaacgttt tcagaacctg tttcagggca gattttgctc cttctttgca
    16921 ctcaacccgc cgtaatcatt tatcgtccgc caggcgacat gacacggtgc tattacctga
    16981 caccagtttg tctgacgctg gcttttaggc ggcacgactc ccgcattact taacgcttta
    17041 ccatttacgc tcacctgaat caggttaatg tagtaaggcg ttgggttagt gagttgtaca
    17101 ccctgcgaat tccgtgtcca gcgtaattgt tgataagctt ccagcgggtc gcccggcaaa
    17161 ccttcgggtc gccagaacag tttaaatacc gagcgcatcg ccacttttac gctctgattt
    17221 tcaactttgc cggatggcac gctggcaatg cttaactcaa atagcgtttc gcgatccaca
    17281 ggcaagatgt cgctttccgt tctcagcaag cgcaacgtgc ctgtagtacc gggcttcagg
    17341 agaataagtg gcggagcggc cagtaatagt gctggcactg tcgacgcttc ccccccccgg
    17401 cccaacgcgt tgggcgattg attttgctgt taataagcca agattcctga ctggtattag
    17461 tcagtaaaat agatatcgat tctctgtctg ccggaaagat aaatcgcgtt ccgccgacca
    17521 ccactcctgc ctgcaattca cccgccacca gcagcgataa aacggcgaaa atcaagagcg
    17581 ctaatctgtt ccacgtatta gtcataatga agtacaaatg tcgcgctggc attcacttta
    17641 cccgactgca catcgctatt gacaggtcgc aattgcgcat aaaaagttaa tggcacctta
    17701 ccgctatgta ggctgtagtc tttactcgcc tggttaagcg gaatcaatat cttcgcatca
    17761 tccagaatcg caattgccag cccactggct gcatccgtac cactttcaat ggacagcatt
    17821 tcgctctgca ctccgtcggg ggttcctgaa aaggtgacat caaccgtgct gacatctgtt
    17881 ccgcaatcct gcaaattaat gatgaaagat ttctccgggc cataattccc atgaaaaaaa
    17941 cgtgtcagcg agacatcgcc taactgcaca ttgatttgct tgctatcctg tgccagcacg
    18001 catgtattac ggcgaaacga tccgctaatg ttgaccgtgc catcatgggc aaatagctca
    18061 cagccccccg ttaatcccag taagaacaga atccagatta tccgtttcaa catcactcct
    18121 tcactggtaa accagatcaa aatagagaac cgccgtcgca ttaccgcccg ttgctcccgc
    18181 ctttgtggac ttataacgaa gttgatattt gagtgtgtta tccccggctt ttaagggcgt
    18241 aagaggctgg acctgattga gcgggatttc ttgctgactt tgcgcatcaa gaatttgcac
    18301 ggcaatcccc gttgccatac caccgcttcc gcctgtgtca gttagtttga gcagtgtcgg
    18361 cgcaagggtg tcagcctcgc cgctaaaaag gacgtccgcg ccagtaatac cagcggcaca
    18421 accggtgata tcaatattca aatcagcagc agtgcttacg gtattcgcgg cctgaaaatc
    18481 actgatatta aaataaccaa tatgaatatt ttgtaatgca cttttggtgg cgacatcaca
    18541 gccctgatcg accacattcc cataaatatg catactgact tgttcaggcc cagccatccc
    18601 gacatggatg ttaagaaggc taaagaacaa aataacgcac tgatatatcg tttttttctt
    18661 catacgttca cgcgttaatc aaaatttatt ctcaccgttg ccgtggcttc aaatcgcccg
    18721 gtttctggtg gtacgcctgt cgtgctgact ggatagacat tcattcgcgg atactgtctg
    18781 catcatcagg aatagagagc ggtagtgtcc caccagcagg ggaaataatg ttgttctggc
    18841 tatcggtcac caccacgccc acgccaggac gtgacgtctt aatcgcctgg ggatagctgg
    18901 ggtctgaggt tgccgtcaga ctcagattga cgttaactaa ccctgcgttt cccgtgttac
    18961 acctgaccgg aattgcaagt tctgccgttc tggccccaag aggcttatgc cctacaacac
    19021 gaaagttagc cgcaaatacg tcgcccagat caacctcgat tgtttctccg gcattgacgg
    19081 aacatgagcc aagtgaattg atcgtaccag acaggtaata cactaaaacc ggtgtaccag
    19141 tagtcgtgca ggaatcacct gcgggtatgt tgtagcattc aaataatcgc gccagcgcca
    19201 taggaggaat gacgaccgaa ccaacgaacg gctgcaatat tttcagggaa aagctaccgc
    19261 tattaccgct agccccctgg ccacctaaac gtagaccgcc gggatcgcaa atatgccatc
    19321 gtgtatctgc ccctgtgccc acgttttcga aaggaaccgc agtaggagaa ctgcctttaa
    19381 cctgcaataa aactttggcg gcaagatagt cattaatttt gtaccagttg tcgcctaaat
    19441 agactaacgg tgaatccgcg gcgaatgccc atcggccact tctgacattg tctttatcgc
    19501 aatcacagga aacataataa tcattcgatt cgttccactg ataaaatttg tctttaacta
    19561 ctccagccgt attttcttct tcatccagag ttttaacgcc aaaattgacg ccaatatacg
    19621 gattcccctg agtggtatga cactgcccga tataagcagc agtttgagcg caccatagca
    19681 ttaagacggc ggcaaaaaat agtcgtagta ataacacgca tttttctcca aagattatct
    19741 gcatatggca tccattcgcg taatcgcaga gttaatttgt tgttcggtaa gctggtaact
    19801 gatatgacaa atttgatcgg gagcttcgcc ccagcgtaca gttatcgctc cctgagcggg
    19861 caaaccggac atatagagcg agccatcatc atcaataatg ccgctattag tctgtatgcc
    19921 gtccagcgtc gctatcgcgc caaaacgcag cggtataccc tgctttgatg ttttcattaa
    19981 tacccttgct cccgcatggg cattgaactc cgccagaacc aacgcccctt tggtgggaac
    20041 cacgttgact actgcatttt caagatccac gttacgtttt aacgatgccg catcaagtgc
    20101 gattcgattt tcccgataag aagtggcata ggggacaacg gtatatccgc gccagtcagt
    20161 tttcagcccg gtgctgttcg ccacacgagt attctctgcg cctggtgctg caatcagcac
    20221 gttagtttcc cccagcgatt gccccagggt aatgccctgt gaatgggcaa ctaaactgcc
    20281 tgaaagagcg tagttcagtt gttgttgact gccgttatcg ctgtagttgt agcccactcg
    20341 ggcatctgca aacgcccctt tgtagtccat gctggcgcta ccattagccg ttttcccctc
    20401 gctgttatat ccctgttgca cgctgtagct caggttaccg tcatcgagta gtgtctccgt
    20461 taaacccagg ttttgtgtaa tgtgcccttc gttatcgata ctggtgttag aggtcatcca
    20521 ggcgtaattt tttttcctgg tatagctatc attacttctg gggagcaaca gattgagcgg
    20581 cagggaaaaa tttagtgcaa acacctgatc agcatcaggt tgaccacggg acttactgta
    20641 gttccaggaa acccccagcg agagatcttt aatttgcgtg ttgtagccaa actgtaatag
    20701 ccgatcctgt tgatcggtat gccagtaagt ttgctggcta ccacttaaat aaaacaaacc
    20761 gtactcgcct aattgctggg agatattgac ctgcagtttg ccacgtttgg tgtaaaacaa
    20821 attgtaataa cgcgaccaca tcggcgtatc ttcatcatca ccgtcattaa attcgtagcc
    20881 atccatatgt ttatacatgg tgtcggaaag ggtgtagaaa cccgacgtcg aatagcgata
    20941 gcccatcaat tgaaaattag tgcccaattg gttgagcgtt ttggcataca gaaaacgata
    21001 tgactgacct ttatgttcgc tttggtctgc cagcgtactc ttcgcctggg tcgcatcgaa
    21061 cgaaattgct ccgaaatcgc caaggttaaa tcccagacca aacatggcgg cacggtaata
    21121 ttcagcatat tgtccaccac cataccatgt cgttccccac ggtccgcccc actgcaacgt
    21181 ggtctgggca aatttgctct cctgctgttc attactattg gttctgtatt tcgccagtgt
    21241 caccgcgtat ttgattcgcc cctgacgctg gagtaatggc acgctggaaa acggtacgct
    21301 gtagctattg acgctgccgt ccgcttcttt gatttcaact aacaaatcac cgctcgacga
    21361 cgtggaatag agatcactaa tttcaaaagc accaggcgat acataagttt ggtaaatggt
    21421 gtaaccattt tgcttaatcg ttatctgggc attgcttttg gcgataccac gcaccacagg
    21481 cgcaaaccct ttcaggctgt ctggcagcat gttgtcatct gacgccagct gcacaccacg
    21541 aaagctgaca ctatcgaaaa aatcgcctgc agtataatca tcacctacgg tcagttcgcc
    21601 ttttagtgga ataaccgccc gctgcaaata gctgctgaga ttcttccatt ctgcggtttg
    21661 gccactactg cgactccatg ttgaattgtt gcgcaatctc catgggccta aattaacgcc
    21721 actgtttaaa ttcagaaaat agctgtcgcc agaatcactg tctgcgctgc tatgaatact
    21781 gttagccccg ctaaatagcg cgttgatccc ttcatcccaa cgttcagggg ggacataacc
    21841 acgggcatta cttttgatgg cgatttgcgg tactgaaatt tccagacgga gtttattcac
    21901 attaaacgtt acgctggcat ccggaataat ctccgccaat ggcacacaca cttgtttgtc
    21961 cagttgttta aaagccggaa acgcatcagt attaacaccc attgcatcca ggctttcagt
    22021 cgtcaaacag gcggcaatcc ccttctctgg cgttatctca acaaaattga cgttgcgcgt
    22081 atctacaatt gtctgattca gaacaatggt aatgactcca acttactgat agtgttttat
    22141 gttcagataa tgcccgatg
//
LOCUS       DAJTQY010000061        21507 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.061, whole genome shotgun sequence.
ACCESSION   DAJTQY010000061 DAJTQY010000000
VERSION     DAJTQY010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21507
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.061"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(37..2106)
                     /gene="glyS"
                     /locus_tag="OO349_002493"
     CDS             complement(37..2106)
                     /gene="glyS"
                     /locus_tag="OO349_002493"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HCR5867525.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2116..3027)
                     /gene="glyQ"
                     /locus_tag="OO349_002494"
     CDS             complement(2116..3027)
                     /gene="glyQ"
                     /locus_tag="OO349_002494"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HCR5867526.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3122..3421)
                     /locus_tag="OO349_002495"
     CDS             complement(3122..3421)
                     /locus_tag="OO349_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HCR5867527.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            3596..4591
                     /gene="wecH"
                     /locus_tag="OO349_002496"
     CDS             3596..4591
                     /gene="wecH"
                     /locus_tag="OO349_002496"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HCR5867528.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4633..5070)
                     /locus_tag="OO349_002497"
     CDS             complement(4633..5070)
                     /locus_tag="OO349_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaB family inner membrane protein"
                     /protein_id="HCR5867529.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(5116..5457)
                     /locus_tag="OO349_002498"
     CDS             complement(5116..5457)
                     /locus_tag="OO349_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5867530.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(5626..7080)
                     /gene="xylB"
                     /locus_tag="OO349_002499"
     CDS             complement(5626..7080)
                     /gene="xylB"
                     /locus_tag="OO349_002499"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HCR5867531.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(7152..8474)
                     /gene="xylA"
                     /locus_tag="OO349_002500"
                     /pseudo
     CDS             complement(7152..8474)
                     /gene="xylA"
                     /locus_tag="OO349_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
     gene            8840..9832
                     /gene="xylF"
                     /locus_tag="OO349_002501"
     CDS             8840..9832
                     /gene="xylF"
                     /locus_tag="OO349_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5867532.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKKA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKESEL"
     gene            9910..11451
                     /gene="xylG"
                     /locus_tag="OO349_002502"
     CDS             9910..11451
                     /gene="xylG"
                     /locus_tag="OO349_002502"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HCR5867533.1"
                     /translation="MSYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRYQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTAIYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            11429..12610
                     /gene="xylH"
                     /locus_tag="OO349_002503"
     CDS             11429..12610
                     /gene="xylH"
                     /locus_tag="OO349_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HCR5867534.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            12688..13865
                     /gene="xylR"
                     /locus_tag="OO349_002504"
                     /pseudo
     CDS             12688..13865
                     /gene="xylR"
                     /locus_tag="OO349_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XylR"
     gene            complement(13973..14797)
                     /locus_tag="OO349_002505"
     CDS             complement(13973..14797)
                     /locus_tag="OO349_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HCR5867535.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            15117..17147
                     /gene="malS"
                     /locus_tag="OO349_002506"
     CDS             15117..17147
                     /gene="malS"
                     /locus_tag="OO349_002506"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HCR5867536.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGKYTLQIDTRSGTPT
                     LMISIQNAAEPVANLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDTSAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTSYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIEGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASSALREWKKTNPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            17325..18578
                     /gene="avtA"
                     /locus_tag="OO349_002507"
     CDS             17325..18578
                     /gene="avtA"
                     /locus_tag="OO349_002507"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HCR5867537.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEGRPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(18729..19202)
                     /locus_tag="OO349_002508"
     CDS             complement(18729..19202)
                     /locus_tag="OO349_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCR5867538.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTPVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLQQQPV"
     gene            complement(19304..20152)
                     /gene="yiaJ"
                     /locus_tag="OO349_002509"
     CDS             complement(19304..20152)
                     /gene="yiaJ"
                     /locus_tag="OO349_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HCR5867539.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLSAIT"
     gene            20353..21351
                     /gene="yiaK"
                     /locus_tag="OO349_002510"
     CDS             20353..21351
                     /gene="yiaK"
                     /locus_tag="OO349_002510"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HCR5867540.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            21363..>21507
                     /locus_tag="OO349_002511"
     CDS             21363..>21507
                     /locus_tag="OO349_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HCR5867541.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRATNFNVLEPGVVEIDGKN
                     IYAQ"
BASE COUNT         5403 a         5487 c         5282 g         5335 t
ORIGIN      
        1 gcctgccaga tggcaggctt tttaataacg gcgttattac tgcaacagcg aaatatccgc
       61 aacgcgcagg aacagttcgc gcagtttctc cagcatggtc agacggttga gacgcaattc
      121 tttgtcatca accatgacca tcactttatc gaagaaagca tcaaccggct cacgcagctc
      181 tgccagttcg accagcgcat cctggtaacg accttccgca aagtacggct ccagcttgtc
      241 acgtagcacc acaacctgca tcgccagttt aatttcttcc ggctctttca gggtagaggc
      301 attcacgcga tcgctcagca cttcgtcaga tttcgccagg atgttagata cacgtttgtt
      361 cgccgccgcc agtgctgcag ctgcttccag ggtacggaag tgcgataccg ctttcatgcg
      421 ggcatcgaaa tcagccggac gagtcggacg acgcgccagt accgcctgga tggtgtcaac
      481 ggtataacct tcgtcctgat accaggcgcg gaagcgaccg agcataaagt cgataacatc
      541 atcaactacg ttggcattag tcagcttatc gccatacaga cgcaccgctt cttcggtcag
      601 cgtttgcaga tcaaggttga ggttcttctc aacgataatt cgcagcacgc caagtgcggc
      661 acgacgcagc gcaaacgggt ctttgtcgcc tttcggatgc tgaccgatac cgaagatacc
      721 cgccagagta tccatcttat cagcaatcgc cagcgcacaa gctaccgggt tggacggcag
      781 gtcatcaccg gcaaagcgcg gctgatactg ctcattcagc gcgacggcta cgtcttccgc
      841 ttcgccatcg tgacgcgcgt agtgcatccc cataacgcct tgggtgtcgg tgaactcgaa
      901 gaccatgttg gtcatcaggt cacacttaga cagcaggccc gcacgggttg cgtggttaac
      961 gtcagcgcca atctgttcgg cgatccagcc agccagcgcc tggatgcggt cagtcttgtc
     1021 acgcagcgta cccagctgtt gctggaacag cacggtttgc aggcgcggca ggttatcttc
     1081 cagacgtttt ttgcggtcgg tgttgaagaa gaactcggca tccgccagac gtggacgaac
     1141 gactttctcg ttaccagaga taatttgctg cggatctttc gattcgatgt tggcaacgaa
     1201 gataaagttc ggcagcagtt tgccgtcgtt cgcatacacc gggaagtact tctggtcacc
     1261 tttcatggtg taaaccaggg cttcagccgg caccgcgagg aatttctctt cgaattttgc
     1321 ggtcagaacg accggccact ccaccagcga agccacttct tccagcaggc tttcgcttaa
     1381 gtcagcgtta ccgccaatct tacgcgctgc ttcttcggca tcggctttaa tcttcgcttt
     1441 acgttcttcg taatcggcga tgactttccc gcgctcacgc agaatttccg gatactgatc
     1501 ggcgttgtcg atggtgaatt ccggttcgcc cataaagcgg tggccgcgaa tcacgcgatc
     1561 ggactgaatg cccagaatgg ttgccggaat gactttgtcg cccagcagca gggtcacggt
     1621 gtgcaccgga cgcacgaagt gcacgtcgct tgcaccccag cgcatcagtt tcgggatcgg
     1681 cagtttcgcc agcgaagtcg caaccatatt cggcagcagt gcttcggtgc tttcgccctt
     1741 cacatgggcg cgatacagca gccattcgcc tttatcggta gtcagacgct cagcctggtc
     1801 aacggtaata ccacaaccac gcgcccaacc ttctgccgct ttgctcggtt ttccttcagc
     1861 gtcgaacgcc tgggcaatcg ccgggccgcg tttttcgatt tcacgatctg gttgtgcttc
     1921 cgccaggtta gctactttca gcgccagacg acgcggagca gcaaaccatt gaacggtgcc
     1981 gtgtgcgagg cctgcgttat ccagctccgc agtaaagttc gcagcaaagg actctgccag
     2041 gctgcgcagt gcttttggtg gcagctcttc agtgccgatt tccaccagaa aagttttctc
     2101 agacatagcc gcctcttact tatctttgtt gcacatcggg aagccgaggg cttcacggga
     2161 agcgtaatat gcttctgcca ctgctttggt cagggtgcga atgcgcagaa tgtagcgctg
     2221 acgctcggtg acggagatgg ctttacgcgc atccagcagg ttgaagctgt gggcggcttt
     2281 cagaatacgc tcgtaggctg gcagcggcag cggattttcc agcgccagca gctgctgcgc
     2341 ttctttctcg tactgctcaa agcaggtgaa caggaagtcc acatccgcgt attcgaagtt
     2401 gtaagtggac tgctccactt cgttctgatg gaacacgtcg ccgtaggtgg ttttacccag
     2461 cgggccgtcg ctccagacta ggtcgtaaac gctgtctacg ccctgaatgt acatggccag
     2521 acgttccaga ccgtaggtga tctcgccggt aaccggttta cactccagac caccaacctg
     2581 ctggaagtaa gtgaactgcg tcacttccat gccgtttagc cacacttccc agcccagtcc
     2641 ccaggccccc agagtcgggt tttcccagtt atcttccacg aaacggatgt cgtgaatagt
     2701 cgggtccatg cccagctctt tcagagaacc gaggtacagc tcctgaatat tgtccggcga
     2761 tggcttaatg accacctgga actgatagta gtgctgtaaa cggttggggt tttcgccgta
     2821 gcgaccatcg gtcgggcgac gagaaggctg aacataagca gccgccatcg gttctggccc
     2881 cagcgcgcgc agacaggtca ttgggtgaga ggttcccgcg ccgacttcca tgtccaatgg
     2941 ttgaacaatg gtgcagccct ggcgagccca gtaatcctgt aaggtcagga tcaagccctg
     3001 gaaggtcctg gtatcaaact tttgcatatt atttcgtgct ggatacgtgt ggatttaaag
     3061 gaagggatca gtatacccgc tggatggaag atatacagta cgaaacggga aaaagcaggg
     3121 cttaacgcat ggaaagatgc aaaaaatggc catctgcgtc aaaagaacag acaaactgct
     3181 ctttacggaa ggtataaccg cgcatttcat agcttccctg gaactgttcg aaggcggtga
     3241 cgtcgatttt ttgctcgccg gtgttataac gttttgccgc ctgatccttg cataacgctt
     3301 ccatattcaa cgaacgcaga ggatcaacct ttacccgttg tgctttctgc acgggtgacg
     3361 gtgtagaaca acccactagc accataagcg ccattgccgg aaagaaagcg ttcatcatca
     3421 ttaaattacc gtctggtttg caggtgagtc ttattattta tatcggtata gtggctgata
     3481 tcttacgaat ttccttaagc ctctataggc tgtagtgata aaactcagat tattccagtg
     3541 cgccaaccaa atgatttatc ggtcagactt tttttaacca gatacagaga cacagatgca
     3601 gcccaaaatt tactggattg ataacctgcg agggatagcg tgtttaatgg tggtgatgat
     3661 tcacaccact acctggtatg tgaccaatgc tcatagtgtt agccccgtca catgggatat
     3721 cgccaatgtt ctgaattctg cctctcgtgt cagcgtgccg ctatttttca tgatttccgg
     3781 ctatctcttt tttggcgaac gcagcgccca gccgcgccat ttcttgcgta tcggcttatg
     3841 tctgattttt tatagcgcaa tcgcactgct ctacattgcg ctgtttacct ccatcaatat
     3901 ggagttagcg ctgaaaaacc tgctgcaaaa gccagtgttt taccacttgt ggtttttctt
     3961 cgcgattgcg gtgatttatc tggtttcacc gctgattcag gtgaagaacg tcggcggaaa
     4021 aatgttgctg gtgctaatgg tggtgattgg cattatcgct aacccaaaca cagtgccgca
     4081 gaaaattgac ggttttgaat ggctgccaat taacttatat atcaatggcg atacttttta
     4141 ctacattctg tatggcatgt tgggccgcgc tatagggatg atggacacac agcataaagc
     4201 actgtcgtgg gtgagcgccg cgctgtttgc gacgggggtt tttattatct ctcgcgggac
     4261 attatatgaa ttgcagtggc gcggaaattt tgccgatacc tggtatcttt actgtgggcc
     4321 gatggttttt atctgcgcaa tcgcgctatt gactctggtt aaaaacacgc tggatacgcg
     4381 taccattcgc ggacttggct taatctcccg ccattcattg ggtatatacg gattccacgc
     4441 cttgattatc catgcgctgc gcacccgggg aattgagctt aaaaattggc caatactgga
     4501 tattatttgg atcttttgcg cgacgttggc agcgagtttg ttactttcta tgctggtaca
     4561 acgaatcgac agaaacagat tagtgagtta agtaaaagcc cgttcacatt ggactgaccg
     4621 ggcttacgtg aattattcgc tgtattcttc ctgggtaacc tgtgtttctt ttggcgggtt
     4681 tattccggca tcacgaatat tcttctgcac cgctactgca ccaaacaagc ttaagaagaa
     4741 agccagtcca taaaaacctt tttcactgag taataatgtc gcattccaca gacctaccat
     4801 cagtagagca acagagataa taaagacagt caggcaggtc atataataaa tggaagtggt
     4861 cggtatgcct tcatacttgt cccgaacggt cttttgataa gacgcagcag aaaacagtcc
     4921 taacaccagt acggcaaaat aatacccttt ttcatttaac tgcatctctg cattccatag
     4981 ccctaacagg taggtaacga taccaccaac gagagctatc catgacacaa tactaaaggc
     5041 cggtgaatag gttgatattt tgttgtccat cacagtattc ctttcattcc tgaatgtgta
     5101 agagctttat gttgctcata ccgagcgaaa caaacgcatt tgaccaaaca agcagacaaa
     5161 aaaggcgatg gggtagatca ccatttcata agcattgata ggtgcattta aaacaccgat
     5221 aaacaagaga ccgatagtga ttaacgcaac taactggcag atatgggtaa aaaaattatc
     5281 cagttgcccg agtttttctg cgcgaagata tgcatagtta ccaaaagttg ctgtcattaa
     5341 cacgccaaga aaatagcctt ttccacttag caatgggcat gagatccata gcccaaccag
     5401 ataaaccagc gccccgacaa caaataatag ttttgccaca gtttttgagg ttttcataac
     5461 gatctccata tctaccagcg atacattacg agtaaccaac gaaagacaaa actgaaaaat
     5521 gccattaaca aatgattttc agaataaatt catactaaat attaattaat tagtgagata
     5581 tatagatgtg aattatcccc cacccggtca ggcaggggat aacgtttacg ccattaatgg
     5641 cagaagttgc tgatagaggc gacggaacgt ttcacgtcgt ggctgataag cggcataacg
     5701 ctgcgcatct ggtagatgcg actgttctaa cggtagctgc ggcaacaact caatgagcga
     5761 tttctctgga ttcgccgcaa tctgcgccag ccttgctgcg cccagtgctg gccccacatc
     5821 cccccccgta cggtaatcga gctgctgacc gctgatatcc gccagcatct gacgccagta
     5881 ctcactacgc gccccgccac caatcaacgt aacactttgc ggtttaatgc cgcaggcatg
     5941 cacgacatcc atgccatctg ccagcgcata acccacgcct tccagcactg ctcgcgccag
     6001 ttcattgggg ccatgttgat gagtcaaacc aaagaaaacc cccttcgcct ggggattatt
     6061 gtgtggtgta cgctcgccgg aaagataagg cagaaaccaa actggctcgg cactttcatc
     6121 agcctgttga gctgcagcga ttaaagctgg gacattgcac aggccggtta atttcgcggc
     6181 ccaatccaga cacgatgctg cactcagcat tacagacatt aaatgccaac gctgcggtag
     6241 cgcatggcaa aagctgtgta cagcgctttc tggcttgctt aagaaccctt cgctgacagc
     6301 aaaatagacc cccgacgtcc ccagcgataa cattgcctga ttagcatcaa ccattcccac
     6361 accaactgca ccagctgcat tgtcgccacc gcctgcgaca actggcaccg tcgccatacc
     6421 ccacgctttc gcaacttcag gtaacaaagc accagtaatt tcgctgcctt cgtataatgc
     6481 gggcatctgg tcacgagata agtcgcaagc ctgcagcatg acgtcactcc agtcacgctt
     6541 tgcgacatcc agccacatgg tgccagctgc gtcagacata tcgctggcaa actcccccgt
     6601 catacgcaga cgcaagtaat ctttcggtaa taatactttg tcgatttgac ggaatatctc
     6661 cggctcatgc cgctgaaccc atagcaattt aggcgcagta aatccgggca tcatcaggtt
     6721 gccggtaatc actcgtgatt gcggaactct cgcttccagc aaagtgcact cttgcgcaca
     6781 gcgcccgtcg ttccacaaaa tggcagggcg caatacccgt tgttgagcat ccagtaaggt
     6841 tgctccatgc atctggccgg caatacccaa tgctttaacg tcctgcagag aatgctgatc
     6901 gcccagagct ttcattgcgc gatcagttgc ctgccaccac tgttccgggt cttgttccga
     6961 ccaaagtgga tgcgggcgcg aaacggtcag cttttccgtt tgcgaagcaa ccacctcacc
     7021 ctgctcgttg agcaaaataa cttttacgcc cgaggtgcca agatctatcc cgatatacat
     7081 atcgatcgtt ccttaaaaaa atgcccggta tcgctaccga taaccgggcc aacggactgc
     7141 acagttagcc gttatttgtc gaacagataa tgatttacca gattttccag ttgctcctgg
     7201 cgaccactct gatgcaccgg agacaaatta tgttcctgag catatttggc taaatctgcc
     7261 agtgacattt ggcctttcag gatttgctgg cccaattcgc tattccagcc ggaataacgc
     7321 tgcgcgatgc gtttatccag ctcgccatct tcaatcatgc gcgctgcaat tttcagcgcc
     7381 agtgccatca tatccatcgc gccgatatga ccgtaaaaca gatcatattt atcagtactt
     7441 tgacgacgta ctttggcatc gaagttcaga ccaccggtgg tgaaaccgcc tgctttgaga
     7501 atttcataca tcaccagcgc attctcttcc acactgttcg ggaactggtc ggtgtcccag
     7561 cccagttgcg catcgccacg gttggcgtcg acagaaccga acaggccaag cgcaatggcg
     7621 gtggctattt catgatggaa agagtgacct gccagcgtcg cgtggttagc ttcaatgttc
     7681 agtttaatct ctttttccag accaaactgt ttcaggaagc catagaccgt cgcggcatcg
     7741 taatcatatt gatgtttagt cggttcttgc ggtttcggtt cgataagcaa cgtgccctag
     7801 aagccgattt tatgtttatg ctcaaccacc atctgcataa agcggcccag ttgttcacgc
     7861 tcctgacgca agtcggtatt taacagcgtt tcgtaacctt cacgaccacc ccacaggaca
     7921 tagttttcac cgcccaattt atgggttgct tccatcgctg taacaacttg cgttgccgcc
     7981 cagctgaaga cttcaggatc tgggttcgtc gccgcacccg cgccatagcg agggtttgta
     8041 aagcagttag cggttcccca cagcagcttc acgccgctct cttcttgctt ggctgccagg
     8101 acatcaacca tttgcgcaaa attattgatg tactctttta acgacgcgcc ctcaggggaa
     8161 acatccacat cgtggaagca ataaaatggc acatgtaact tgtggaaaaa ctcaaatgcg
     8221 acatctgctt tacgcttcgc caacgccagt gcctcaccag gctgctgcca cggacgatta
     8281 aacgccccca caccaaacat gtccgccccg ttccagcaga aggtgtgcca gtagcaggcg
     8341 gcaaaacgca agtgctcttc catacgctta cccaacacca gttcgtcggg attgtagtga
     8401 cggaatgcta acgggtttga ggattttgag ccttcataac gaacgcgatc gagctggtca
     8461 aaataggctt gcatattgaa ctccataatc aggtaatgcc gcgggtgatg gatgatgtcg
     8521 taatattggg cactcccttt caattgctca attatgttat ttcacactgc tattgagata
     8581 attcacaagt gtgcgctcgc tcgcaaaata aaatgggatg atgaaactgg gtaattcctc
     8641 gaagataaaa atgcaataag tacaattgcg caacaaaagt aagatctcgg tcataaatca
     8701 agaaataaac caaaaatcgt aatcgaaaga taaaaatctg taattgtttt cccctgttta
     8761 gttgctaaaa attggttacg tttatcgcgg tgattgttac ttattaaagc tgtcctctaa
     8821 ctacagaagg ccctacacca tgaaaataaa gaacattcta ctcacccttt gcacctcact
     8881 tctgcttacc aacgttgcgg cacacgccaa agaagtcaaa ataggtatgg cgattgatga
     8941 tctccgtctt gaacgctggc aaaaagatcg agatattttt gtgaaaaagg cagaatctct
     9001 cggtgcgaaa gtatttgtac agtctgcaaa tggcaatgaa gaaacacaaa tgtcgcagat
     9061 tgaaaacatg attaaccggg gtgtcgatgt tcttgtcatt attccgtata acggtcaggt
     9121 attaagtaac gttgtaaaaa aagccaaaca agaaggtatt aaagtattag cttacgaccg
     9181 tatgattaac gatgcggata tcgattttta tatttctttc gataacgaaa aagtcggtga
     9241 actgcaggca aaagccttgg tcgatattgt tccgcaaggt aattacttcc tgatgggcgg
     9301 ctcgccggta gataacaacg ccaagctgtt ccgcgccgga caaatgaaag tgttaaaacc
     9361 ttacgttgat tctggaaaaa ttaaagtcgt tggtgaccaa tgggttgatg gctggttacc
     9421 ggaaaacgca ttgaaaatta tggaaaacgc gctaaccgcc aataataaca aaattgatgc
     9481 tgtagttgcc tcaaacgatg ccaccgcagg tggggcaatt caggcattaa gcgcgcaagg
     9541 tttatcaggg aaagtagcaa tttccggcca ggatgcggat ctcgcaggta ttaaacgtat
     9601 tgctgccggt acgcaaacta tgacggtgta taaacctatt acattgctgg caaatactgc
     9661 cgcagaaatt gccgttgaat tgggcaatgg tcaggagcca aaagcagata cctcactgaa
     9721 taatggcctg aaagatgtcc cctcccgcct cctgacaccg atcgatgtga ataaaaacaa
     9781 catcaaagat acggtaatta aagacggatt ccacaaagag agcgagctgt aagcgttacg
     9841 ccccggcgcg gagcgggggc gtgatttctc tccatgccgc gtgaatgaat tggcttaggt
     9901 ggagtcgtta tgtcttatct acttgaaatg aagaacatta ccaaaacctt cggcagtgtg
     9961 aaggcgattg ataacgtcag cttgcggttg aatgctggcg aaatcgtctc actctgtggg
    10021 gaaaatgggt ctggtaaatc aacgctgatg aaagtgctgt gtggtattta tccccatggc
    10081 tcctacgaag gcgaaattat ttttgcggga gaagagattc aggcgagtca catccgcgat
    10141 accgaacgca aaggtatcgc catcattcac caggaattgg ccctggtgaa agaattgacc
    10201 gtgctggaaa atatcttcct gggtaacgaa ataacccaca atggcattat ggattatgac
    10261 ctgatgacgc tacgctatca gaagctgctc gcacaggtca gtttatccat ttcacctgat
    10321 acccgcgttg gcgatttagg gcttgggcaa caacaactgg ttgaaattgc caaggcactt
    10381 aataaacagg tgcgcttgtt aattctcgat gaaccgacag cctcattaac tgagcaggaa
    10441 acgtcggttt tactggatat tattcgcgat ctacaacagc acggtatcgc ctgtatttat
    10501 atttcgcaca aactcaacga agtcaaagcg atttccgata cgatttgcgt tattcgcgac
    10561 ggacagcaca ttggtacgcg tgatgctgcc ggaatgagtg aagacgatat tatcaccatg
    10621 atggtcgggc gagagttaac cgcgatttac cctaatgaac cacataccac cggagatgaa
    10681 atattacgta ttgaacatct gacggcatgg catccggtca atcgtcatat taaacgagtt
    10741 aatgatgtct cgttttccct gaaacgtggc gaaatactgg gtattgccgg actcgttggt
    10801 gccggacgta ccgagaccat tcagtgcctg tttggcgtgt ggcccggaca atgggaagga
    10861 aaaatttata ttgatggcaa acaggtagat attcgtaact gtcagcaagc catcgcccag
    10921 gggattgcga tggtacccga agacagaaag cgcgacggca tcgttccggt aatggcggtt
    10981 ggtaaaaata ttaccctcgc cgcactcaat aaatttaccg gtggcattag ccagcttgat
    11041 gacgcggcag agcaaaaatg tattctggaa tcaattcagc aactcaaagt taaaacgtcg
    11101 tcccccgacc ttgctattgg acgtttgagc ggcggcaatc agcaaaaagc gatcctcgcc
    11161 cgctgtctgt tacttaaccc gcgcatcctc attcttgatg aacctaccag gggtattgat
    11221 attggcgcga aatatgagat ctacaaatta attaaccaac tcgtccagca gggtattgcc
    11281 gttattgtca tctcttccga attacctgaa gtgctcggcc ttagcgatcg tgtactggtg
    11341 atgcatgaag ggaaactaaa agccaacctg ataaatcata acctgactca ggagcaggtg
    11401 atggaagccg cattgaggag cgaacatcat gtcgaaaagc aatccgtctg aagtgaaatt
    11461 ggccgtaccg acatccggtg gcttttctgg gctgaaatca ctgaatttgc aggtcttcgt
    11521 gatgattgca gctatcatcg caatcatgct gttctttacc tggaccaccg atggtgccta
    11581 cttaagcgcc cgtaacgtct ccaacctgtt acgccagacc gcgattaccg gcatcctcgc
    11641 ggtaggaatg gtgttcgtca taatttctgc tgaaatcgac ctttccgtcg gctcaatgat
    11701 ggggctatta ggtggcgtcg cggcgatttg tgacgtctgg ttaggctggc ctttgccact
    11761 taccatcatt gtgacgttgg ttctgggact gcttctcggt gcctggaacg gatggtgggt
    11821 cgcgtaccgc aaagtccctt catttattgt caccctcgcg ggcatgttgg catttcgcgg
    11881 catactcatt ggcatcacca acggcacgac tgtatccccc accagcgccg cgatgtcaca
    11941 aattgggcaa agctatctcc ccgccagtac cggcttcatc attggcgcgc ttggcttaat
    12001 ggcttttgtt ggttggcaat ggcgcggaag aatgcgccgt caggctttgg gtttacagtc
    12061 tccggcctct accgcagtag tcggtcgcca agctttaacc gctatcatcg tattaggcgc
    12121 aatctggctg ttgaatgatt accgtggcgt tcccactcct gttctgctgc tgacgttgct
    12181 gttactcggc ggaatgttta tggcaacgcg gacggcattt ggacgacgca tttatgccat
    12241 cggcggcaat ctggaagcag cacgtctctc cgggattaac gttgaacgca ccaaacttgc
    12301 cgtgttcgcg attaacggat taatggtagc catcgccgga ttaatcctta gttctcgact
    12361 tggcgctggt tcaccttctg cgggaaatat cgccgaactg gacgcaattg cagcatgcgt
    12421 gattggcggc accagcctgg ctggcggtgt gggaagcgtt gccggagcag taatgggggc
    12481 atttatcatg gcttcactgg ataacggcat gagtatgatg gatgtaccga ccttctggca
    12541 gtatatcgtt aaaggtgcga ttctgttgct ggcagtatgg atggactccg caaccaaacg
    12601 ccgttcttga ttttgataaa aattttctca aagccggtta cgtattaccg gttttgagtt
    12661 tttgcatgat tcagcaggaa aagaaccatg tttactaaac gtcaccgcat cacattactg
    12721 ttcaatgcca ataaagccta tgaccggcag gtagtagaag gcgtagggga atatttacag
    12781 gcatcacaat cagaatggga tattttcatt gaagaagatt tccgcgcccg cattgataaa
    12841 atcaaggact ggttaggaga tgtcgtcatt gccgacttcg acgacaaaca gatcgagcaa
    12901 gagctggctg atgtcgacgt ccccattgtt ggggttggcg gctcgtatca ccttgcagaa
    12961 agttacccac ccgttcatta cattgccacc gataactatg cgctggttga aagcgcattt
    13021 ttgcatttaa agagaaaggc gttaaccgct ttgcttttta tggtcttccg gaatcaagcg
    13081 gcaaacgttg ggccactgaa cgcgaatatg catttcgtca gcttgtcgcc gaagaaaagt
    13141 atcgcggagt ggtttatcag gggttagaaa ccgcgccaga gaactggcaa cacgcgcaaa
    13201 atcggctggc agactgacta caaacgctgc caccgcaaac cgggattatt gccgttactg
    13261 acgcccgagc gcggcatatt ctgcaagtat gtgaacatat acatattccc gtaccggaaa
    13321 aattatgcgt gattggcatc gataacgaag aactgacccg ctatctgtcg cgtgtcgccc
    13381 tttcttcggt cgctcagggc gcgcggcaaa tgggctatca ggcggcaaaa ctgttgcatc
    13441 gattattaga taaagaagaa atgccgctac agcgaatttt ggtcccacca gttcgcgtca
    13501 ttgaacggcg ctcaacagat tatcgctcgc tgaccgatcc cgccgttatt caggccatgc
    13561 attacattcg taatcacgcc tgtaaaggga ttaaagtgga tcaggtactg gatgcggtcg
    13621 ggatctcgcg ctccaatctt gagaagcgtt ttaaagaaga ggtgggtgaa accatccatg
    13681 caatgattca tgccgagaag ctggagaaag cgcgcagtct gctgatttca accaccttgt
    13741 cgatcaatga gatatcgcaa atgtgcggtt atccatcgct gcaatatttc tactctgttt
    13801 ttaaaaaagc atatgacaca acgccaaaag agtatcgcga tgtaaatagc gaggtcatgt
    13861 tgtagcgatg atgagaattg tcggcgtcac atcagggaac tcagcacagc cacctgatgc
    13921 gagcaaaagc cttatccgac cgacaaatca gataaggctc gaggaaatga aattacatat
    13981 gcgccgcgat taaccgttgg ttatcctggt acattgcgaa caggtagttg ttataactct
    14041 tccctttggt cgagtagccc ttcagcttgt gaatcatcgc tgtggcagtc acttcctgat
    14101 ccgctttacg cagctgtgca cgcgatttac ggaacgaaga gtaagccggg tgcgtattca
    14161 ggttagtgac ataggcgctc accgattctt tgacagaact aaactgtgag taccctttca
    14221 ctttacccgg cgcattggta caacgtcctt tcatgcattt catgccgaac agattgttat
    14281 tgttgcgcgc cagcttcgac gttccccaac cgctttctgc tgcagccatc gtcgccacca
    14341 tactggtagg gataatgtct acgcgttcaa gcaaggtatt ccacgggatt tttcgcgtat
    14401 taccagacca cttcaccttg tagcgtttgg cgatgtcttt cagacgcgca cgctcagcag
    14461 gtgaccattg gccctgatac tgttttgaaa tcagccagtt acgttctgca gtaatggctg
    14521 cattttggct ggtaatgtaa ggcattacgg tccggagaaa cgcctttttc cttggtgttc
    14581 cggaagggta ttttcgcaaa tcaggaagtg aactactctt tgcactattg cgagaatact
    14641 cttgtttact gcttacctgt ttattactag ctttagttaa gtgggacttt tgactcgctg
    14701 ttgttgtgtg cgtcttcgct aacacctcac tcgaaaacac cagagtgagt aacataagaa
    14761 tcatcgcccc atatcgtcgt atgggagtca aaatcatcag gtctcctggt cggatttaat
    14821 cattccaaca ccttatattt ttcacaaatt tgagagttga atctcaaatc atatcaaaaa
    14881 tagctgtcaa gagcacccca aggaatagtc caaatctgaa actatgtcac gagttaacga
    14941 ttcagattgg cgctaaatcg cagaaaatgt gggggttatc gcaaaattca gccgtttttt
    15001 gcgcgcgatc gctcaccctt gcttctcatc ctgtggactt accgctcagg gatgagtttt
    15061 gtctggctta tcgctggcaa actgtctgaa atcgcagcaa taaggactca tccaccatga
    15121 aactcgccgc ctgttttctg acactccttc ctggcttcgc cgttgccgcc agctggactt
    15181 ctccggggtt ccctgccttt agcgaacagg gaacgggaac atttgtcagc cacgcgcagt
    15241 tgcccaaagg tacgcgtcca ctcacgctaa attttgacca acagtgctgg cagcctgcag
    15301 atgcgataaa actcaatcag atgctttccc tgcaaccttg tagcaacacg ccgcctcaat
    15361 ggcgattgtt cagggacggc aaatatacgc tgcaaataga cacccgctcc ggtacgccaa
    15421 cattgatgat ttccatccag aacgccgccg aaccggtagc aaacctggtc cgtgaatgcc
    15481 cgaaatggga tggattaccg ctcacgctgg atgtcagcgc cactttcccg gaaggagccg
    15541 ccgtacggga ttattacagc caacaaattg cgatagtgaa gaacggtcaa ataacgttac
    15601 aacccgctgc taccagcaac ggtttactcc tgctggaacg ggcagaaact gacacatccg
    15661 cccctttcga ctggcataac gccacggttt actttgtgct gacagatcgt ttcgaaaacg
    15721 gcgatcccag taatgaccag agttacggac gtcataaaga cggtatggcg gaaattggca
    15781 cttttcacgg cggcgattta cgcggcctga ccaacaaact ggattacctc cagcagttgg
    15841 gcgttaatgc tttatggata agcgccccat ttgagcaaat tcacggctgg gtcggcggcg
    15901 gtacaaaagg cgatttcccg cattatgcct accacggtta ttacacacag gactggacga
    15961 atcttgatgc caatatgggc aacgaagccg atctacggac gctggttgat agcgcacatc
    16021 agcgcggtat tcgtattctc tttgatgtcg tgatgaacca caccagctat gccacgctgg
    16081 cggatatgca ggagtatcag tttggcgcgt tatatctttc tggtgacgaa gtgaaaaaaa
    16141 cgctgggtga acgctggagc gactggaaac ctgccgccgg gcaaacctgg catagcttta
    16201 acgattacat taatttcagc gacaaaacag gctgggataa atggtgggga aaaaactgga
    16261 tcagaacgga tatcggcgat tacgacaatc ctggattcga cgatctcact atgtcgctgg
    16321 cctttttgcc ggatatcaaa accgaatcaa ctaccgcttc tggtctgccg gtgttctata
    16381 aaaacaaaac ggatactcac gctaaagtca tcgaaggctt tacgcctcgc gattacttaa
    16441 cccactggtt aagtcagtgg gtccgcgact atgggattga tggttttcgg gtcgataccg
    16501 ccaaacatgt tgagttgccc gcctggcagc aactgaaaac cgaagccagc tccgcgcttc
    16561 gcgaatggaa aaaaactaac cccgacaaag cattagatga caaacctttc tggatgaccg
    16621 gtgaagcctg gggccacggc gtgatgcaaa gtgactacta tcgccacggc ttcgatgcga
    16681 tgatcaattt cgattatcag gagcaggcgg cgaaagcagt cgattgtctg gcgcagatgg
    16741 atacgacctg gcagcaaatg gcggagaaat tgcagggttt caacgtgttg agctacctct
    16801 cgtcgcatga tacccgtctg ttccgtgaag ggggcgacaa agcagcagag ttattactat
    16861 tagcgccagg cgcggtacaa atcttttatg gcgatgaatc ctcgcgtccg ttcggtccta
    16921 caggttctga tccgctgcaa ggtacacgtt cggatatgaa ctggcaggat gttagcggta
    16981 aatctgccgc caacgtcgcg cactggcaga aaatcagcca gttccgcgcc cgccatcccg
    17041 caattggcgc gggcaaacaa acgacacttt cgctgaagca gggctacggc tttgttcgtg
    17101 agcatggcga cgataaagtg ctggtcatct gggctgggca acagtgactt ttccggcttc
    17161 ccgttcgtca gtacctcggg aagccgccaa ccaggataaa atgtcagccc taatcagcgt
    17221 tgcgggataa agcaccgctc actcttcaac agaccgattt gcaccccagc aaatgtagcg
    17281 ttattgttac cttccttgct acagagttcg acagatatcc cgctatgaca ttctcccttt
    17341 ttggtgacaa atttacccgc cactccggca ttacgctgct gatggaagat ctgaacgacg
    17401 gtttacgcac gcctggcgcg attatgctcg gcggcggtaa tccggcgcag atcccggaaa
    17461 tgcaggacta cttccagacg ctactgaccg acatgctgga aagtggcaaa gcgactgatg
    17521 cactgtgtaa ctacgacggt ccacagggga aaacggagct actcacactg cttgccggaa
    17581 tgctgcgcga gaagttgggt tgggatatcg aaccacagaa tattgcacta acaaacggca
    17641 gccagagcgc gtttttctac ttatttaacc tgtttgccgg acgccgtgcc gatggtcggg
    17701 tcaaaaaagt gctgttcccg ctggcaccgg aatacattgg ctacgctgac gccggactgg
    17761 aagaagatct gtttgtctct gcgcgtccga atattgaact gctgccggaa ggccagttta
    17821 aataccacgt cgattttgag catctgcata ttggcgaaga aaccgggatg atttgcgtct
    17881 cccggccgac gaatccaaca ggcaatgtga ttactgacga agagttgctg aagcttgacg
    17941 cgctggcgaa tcaacacggc attccgctgg tgattgataa cgcttatggc gtcccgttcc
    18001 cgggtatcat cttcagtgaa gggcgcccgc tatggaatcc gaatatcgta ctgtgcatga
    18061 gtctttccaa gctggggctg cctggctccc gctgcggcat tatcatcgcc aatgaaaaaa
    18121 tcatcactgc catcaccaat atgaacggca ttatcagcct ggcacctggc ggtattggtc
    18181 cggcgatgat gtgtgaaatg attaagcgta acgatctgct gcgcctgtct gaaacagtca
    18241 tcaaaccgtt ttactaccag cgtgttcagg aaactatcgc catcattcgc cgctatttac
    18301 cggaaaatcg ctgcctgatt cataaaccgg aaggagccat tttcctctgg ctatggttta
    18361 aggatttgcc cattacgacc gagcagctct atcagcgcct gaaagcacgc ggcgtgctga
    18421 tggtgccggg gcacaacttc ttcccgggac tggataaacc gtggccgcat acgcatcaat
    18481 gtatgcgcat gaactacgta ccagagccgg agaaaattga ggcgggggtg aagattctgg
    18541 cggaagagat agaaagagcc tgggctgaaa gtcactaaaa attgcctgat gcgctacgct
    18601 tatcaggcct acatgatttc tgcaatatat tgaatttgga agaatttgta ggccggataa
    18661 ggcgtttacg ccgcatctgg cataaacaaa gcgcactatt ccggcgttga gaaacgccgg
    18721 aaaacgtttc aaacaggctg ctgttgtagc cgatgccgct gcactttctc gacatccatg
    18781 cactgcaacg cctttgtcgg gcaggcttca acacaggccg gccccgtctc ccgatgccag
    18841 cacagatcgc acttaatcgc cctcgccttt ttgcgcgaag aaacgacctc catcgcacca
    18901 aacgggcaag ccagcataca gcttttacag ccaatgcaac gtgtttgttc aacgaaaata
    18961 tgcccatgtt cgcggcttat cgcgtcaaca gggcagacat tcgcgcacgg tgcatcttca
    19021 cactgatgac aggctactgg cgtggtccag ctgtggtctt taatgacacg aatacgggaa
    19081 ataaactcgt ctggtgacaa cgcagcgcaa tcctgattct cctgatgcga cactgcgcaa
    19141 gccacttcac aggtacggca accgatacat ttcgtcgcat ccgcaataat aaaccggttc
    19201 atgatcattc ctttgcacta aaacagcaga gtgccagaga gcgcgtccct actgctttga
    19261 tccaacgaag gaaatgttaa agttttggca cttgtccaaa gcgttatgtg atcgcgctca
    19321 ggtcatccct gacagtaaat cccagttcat tagaaatagc ctgcgcggtt tcacgcagtg
    19381 gtttcaggag atttttctct cccacctgtt tcagacgtga tgtcgaaagc gaaatcgaca
    19441 cggcgtacgg cacccgccca tgaatatcaa acaccggaac agcaatacag gagacgccga
    19501 gttcgttttc ttctctgtcc atcgccgctc cgctttcacg aatgtgcgcc agttcgtcga
    19561 acatcgcggg cagctcggta atggtattgc gggttaacgg ctggatctca tgctgatggt
    19621 tttcccagta tgacttcacg tagtccgggt gaccaaacgc catgtagatc ttgcccattg
    19681 cggaacagta gagcggcata tgctggccaa tataggcacg ggttcgcagc atcccggttg
    19741 tgggttccag cttataaatc aaaatagcgt gatcgtcttc gcggctggag aagttaatgg
    19801 tttcaccagt ggcgatgttc agtgcctcaa gatgcggagc ggcgatatga atgatattca
    19861 gcgaagacag cgccttctgc ccgacggcaa taaatttggt agtcaggcgg taactccctg
    19921 cggcgggcgc ggtggtcaca tagccgcagg actgtaatcc ctgcaataag cgatggacgg
    19981 tactcttatt taaaccagcc agctccgaaa gatgcgccaa cggacaaccg tttggatagt
    20041 tgctcaaaat ctcaatcagc atcaacccgc gaaacagact ctggcttccg gctggacgct
    20101 ctttttcctg cgccatctcg ttctcttttt ttcccatcac ttctttcccc attttgtcgc
    20161 gtcctgatgg tagcgcaaag tgtgccgtag ttcacgatct cgacagataa tttataacca
    20221 cttgattttt atgtcttttg aaattcatca atcagattgc tttgttaaaa agtgatcgat
    20281 atatttgaaa tctagttccg catattgaaa ttttaagcca aaaaagcgat caaaaaaaca
    20341 aggaagccta ggatgaaagt gacatttgag cagttaaaag cagcctttaa tcgggtctta
    20401 atttcacgcg gcgttgacag cgaaacggct gacgcctgtg cagagatgtt cgcccgcacc
    20461 accgaatccg gcgtttattc tcacggcgtt aatcgtttcc ctcgtttcat tcaacaactg
    20521 gaaaacggcg atatcattcc tgatgcccaa cccaaacgta taaccagcct cggcgcaatt
    20581 gaacagtggg atgcccagcg ttcgatcggc aacctgacgg cgaaaaagat gatggatcgc
    20641 gccattgaac tggctgccga tcacggtatt ggtctggtgg cacttcgtaa tgccaaccac
    20701 tggatgcgcg gcggcagcta cggctggcag gcggcggaaa aaggctatat tggcatttgc
    20761 tggaccaact ccatcgccgt aatgccgccg tggggcgcaa aagagtgtcg catcggcact
    20821 aacccgctga tcgtcgccat tccttccacg ccgatcacca tggtcgatat gtcgatgtcg
    20881 atgttctctt acggcatgtt agaagttaac cgcctggcag gccgtcagct cccggtcgat
    20941 ggtggctttg atgatgaggg caatttgacc aaagaacctg gcgttatcga gaagaatcgc
    21001 cgcattttgc cgatgggcta ctggaaaggt tctggcatgt cgattgtgct ggatatgatc
    21061 gctactctcc tttccgacgg cgcatcggtt gccgaagtca cccaggacaa cagcgacgaa
    21121 tacggcattt cacaaatttt tattgccatt gaagtggaca agcttatcga cggtcccacc
    21181 cgcgatgcca agctgcaacg tatcatggat tacgttacta ccgctgaacg cgctgacgaa
    21241 aaccaggcca tccgcttacc cggccacgaa tttactaccc tgctggccga aaaccgccgt
    21301 aacggcatca ctgttgatga cagcgtgtgg gccaaaatcc aggcgttatg aggagataag
    21361 tcatgatttt tggacatatc gcgcagccca atccgtgccg tttgcccgcc gccattgaaa
    21421 aggcgctcga ttttctgcgc gctaccaact tcaacgtcct ggagccgggc gttgtcgaaa
    21481 tcgacggcaa gaatatttat gcgcaaa
//
LOCUS       DAJTQY010000062        21387 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.062, whole genome shotgun sequence.
ACCESSION   DAJTQY010000062 DAJTQY010000000
VERSION     DAJTQY010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21387
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.062"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(<1..134)
                     /locus_tag="OO349_002512"
     CDS             complement(<1..134)
                     /locus_tag="OO349_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5867542.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     "
     gene            complement(236..1216)
                     /locus_tag="OO349_002513"
     CDS             complement(236..1216)
                     /locus_tag="OO349_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HCR5867543.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGATSVRFYPRYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEQGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYASAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(1224..1934)
                     /locus_tag="OO349_002514"
     CDS             complement(1224..1934)
                     /locus_tag="OO349_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HCR5867544.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(2151..3974)
                     /gene="typA"
                     /locus_tag="OO349_002515"
     CDS             complement(2151..3974)
                     /gene="typA"
                     /locus_tag="OO349_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HCR5867545.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            4347..5756
                     /gene="glnA"
                     /locus_tag="OO349_002516"
     CDS             4347..5756
                     /gene="glnA"
                     /locus_tag="OO349_002516"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HCR5867546.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            5929..6978
                     /gene="glnL"
                     /locus_tag="OO349_002517"
     CDS             5929..6978
                     /gene="glnL"
                     /locus_tag="OO349_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HCR5867547.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            6990..8399
                     /gene="glnG"
                     /locus_tag="OO349_002518"
     CDS             6990..8399
                     /gene="glnG"
                     /locus_tag="OO349_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HCR5867548.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAQAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRTKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            8511..8621
                     /locus_tag="OO349_002519"
     CDS             8511..8621
                     /locus_tag="OO349_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HCR5867549.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(8850..10223)
                     /gene="hemN"
                     /locus_tag="OO349_002520"
     CDS             complement(8850..10223)
                     /gene="hemN"
                     /locus_tag="OO349_002520"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HCR5867550.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFHFNTDAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVYEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPLEKQW
                     DLHFADYFEEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(10412..10921)
                     /gene="yihI"
                     /locus_tag="OO349_002521"
     CDS             complement(10412..10921)
                     /gene="yihI"
                     /locus_tag="OO349_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HCR5867551.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            11503..12135
                     /locus_tag="OO349_002522"
     CDS             11503..12135
                     /locus_tag="OO349_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HCR5867552.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKALLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(12517..15303)
                     /gene="polA"
                     /locus_tag="OO349_002523"
     CDS             complement(12517..15303)
                     /gene="polA"
                     /locus_tag="OO349_002523"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HCR5867553.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKTAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISHERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKELGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            15667..16599
                     /locus_tag="OO349_002524"
     CDS             15667..16599
                     /locus_tag="OO349_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCR5867554.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGQDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
     gene            complement(16640..18070)
                     /locus_tag="OO349_002525"
     CDS             complement(16640..18070)
                     /locus_tag="OO349_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HCR5867555.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGIEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(18225..18851)
                     /gene="dsbA"
                     /locus_tag="OO349_002526"
     CDS             complement(18225..18851)
                     /gene="dsbA"
                     /locus_tag="OO349_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HCR5867556.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEEK"
     gene            complement(18868..19854)
                     /gene="srkA"
                     /locus_tag="OO349_002527"
     CDS             complement(18868..19854)
                     /gene="srkA"
                     /locus_tag="OO349_002527"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HCR5867557.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAQQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRHWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(19931..20200)
                     /locus_tag="OO349_002528"
     CDS             complement(19931..20200)
                     /locus_tag="OO349_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HCR5867558.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDFNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            20270..20854
                     /gene="mobA"
                     /locus_tag="OO349_002529"
     CDS             20270..20854
                     /gene="mobA"
                     /locus_tag="OO349_002529"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HCR5867559.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLDLNGKPLWQHVADALK
                     TQLSHVVVNANRHQEIYQAGGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            20836..21363
                     /gene="mobB"
                     /locus_tag="OO349_002530"
     CDS             20836..21363
                     /gene="mobB"
                     /locus_tag="OO349_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HCR5867560.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLHFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT         5233 a         5365 c         5143 g         5646 t
ORIGIN      
        1 atttgaccat tggtcaaatg gaaatattcc tgcatcggga tataaaacgc atctttcagc
       61 gacggtaatt tataaattgt accgccacac agtgttaata gactaaataa cgcccatttc
      121 tttttcgtga gcataacatc ccctaagcga tttttaaaat cactgtttta aaggcaataa
      181 gataccgacc attcatatgg aatggtttta ttaactgcgt atgagttaaa acttattagt
      241 ggttcagaag atggtttgcc tcttgtttga tttgttgtag tacgttacgc acatgattta
      301 tctgattaat tgcatcttct ttttctttac gtaaccgcgc atccagtaaa tgattttctg
      361 gtaggggtgt ttcactcatc tggcgataag ggatccacgg attatcatct tcgtcttcaa
      421 agcggaacgc cgacgcatag taatcaacct cttcttccag gccatatgcc gtcacctgcg
      481 gctcatcatc gccaaggcag ataaggtgcg ttaataacgc tttgaatggc atatcaccct
      541 gacctgaaat acaggcttta tgacccaggc caccctgttc tttaacgatc aaggcatctt
      601 taatatggac ctgggtaata tgcggtgcca tcgtttttaa ggcgtcgatg ggatgctcat
      661 ttgcattgat catgttagca aaatcaaaca gtaaggaaag agactccatc tcactttctt
      721 tgaccagcga caccagctca tgacttttta aatcttcatg ctgctcaatc gtaaaagtca
      781 ggccgctgtc ctgatacgtt tcccgtacat aggcaatgtc gttagcgata atcgataata
      841 cgtcgcgcaa attaccttca taacgtggat aaaaacgtac ggacgttgcc ccagttttca
      901 acgcgatggc gacggcttcg tcaatagatg ccttatctga agcgctggtt tcaatatgaa
      961 tatcaaggtt cagacggcgg gctttatccc caaaggcaga gagttcttta tcgtccatat
     1021 taccaagaga aaaacgctcg ccatccagaa catggatttt gacgccacgt agattatttt
     1081 cacttgcgat atcaagtaaa tcagcaggca acactctttc cagccgcata tttaaatgaa
     1141 aggcataagc atgtaaataa agaggaagag tgtcgacacg ttgtagaatc tttcttgcgt
     1201 tatttattgt caccataaaa cacctacaga ttaatgatga ggtctacggc ctcaaatcgc
     1261 caatattcaa tatctatctg gacaatattg ccttgctggt cggcgcgatg cttttcaatt
     1321 aagatcgctg gcatacccgc agaaccgcca agatacttgc tgatatcgcc cggcattctg
     1381 acgggtttga aaatcaattt ttttactacc gtctcttttt ggtagcactt ttcccagact
     1441 gcagaaaatg agtggttttc aagttgttca ataaaacccg gagcaacttc aggattaata
     1501 tatgtttcgt gatagaaaac tttatgtcct tccagcgccc cccagccggt gatgcgatag
     1561 agttcagttg atggcgtcac tgcaatcagt ggcgcgagcg tctcgggaat atcgctggta
     1621 cggtttttct cggtaaaccc ccaagaaggc tctcgccctt gctcaattgc ggcccgctga
     1681 aagctcgccg acagctccgg actgtaatta aatcgcggct gggtcacgaa ccacccctta
     1741 cgatccttac ggaagatttt ggattctgcc tcgaggttca acaacgcctg gcgcagcgtc
     1801 atacgtttaa tgcccagcag ttctcccagc tctctttctg aagggagttt tccacccggc
     1861 gtggtgatgc catctttcaa ccaccgatcc agtttctctt ttgcattttc tacggtggat
     1921 tgattctccg ccattgttta atccttttgg tttaaaccag atgaagcatc ttacgcttac
     1981 gccaaaaaag aaagcaatta acggtgtatg actttgcaga taattgtgac aaagctctct
     2041 attgacgtaa tcacagactt ataagggaga tactcgcatt ggtgtgcgct cacgacaggt
     2101 aaaaaaaaac ctgccatcgc tggcaggttt tttatgacta aaaaacgaaa ttaatcgtct
     2161 ttcggtgcgc ggttggcgcg gcgacgatcg ttttccgtca ggtgacgttt acgaatacgg
     2221 atagaggtcg gagtcacttc taccagttcg tcatcatcga tgaactccag agcttgttcc
     2281 agagtcatgc ggataggcgg aaccagaaca acagcttcgt cagtaccgga agcacgcatg
     2341 ttggtcagtt tcttaccggt caggcagttt acagtcaggt cgttagagcg gctatgaata
     2401 ccgataatct gaccttcgta aacttctgca ccgtgaccga ggaacagctt accgcgatcc
     2461 tgcagaccga acagcgcgaa cgcgacagct ttaccctgac cgttagagat cagtacaccg
     2521 ttctgacgct gacccacttc gcccggacgt acgtcgtcgt agtggctgaa ggtggagtac
     2581 agcagaccag taccggaagt catggtcatg aactcagaac ggaagccaat cagaccacgg
     2641 cttgggatca cgtagtcgag acgtacgcgg cctttaccgt ctggattcat gtttttcagg
     2701 tcgcctttac gttcgcccag cgcctgcatc acagaaccct gatgctgttc ttcaacgtcc
     2761 agcgtcacgt tttcatacgg ctcttgttta cgaccgtcta tttcacggaa gattactttc
     2821 ggacgggata ccgccagttc gaaaccttca cgacgcatgt tttcgataag aacagagagg
     2881 tgcagttcac cacgaccaga aacgcggaac gcatcggcgt cttcggtttc ttctacgcgc
     2941 agcgcaacgt tgtgtaccag ttctttgttc agacgatcga ggatctgacg agacgttacg
     3001 aacttacctt ctttaccgca gaacggcgag gtgttaacgc agaagaacat agaaacggtc
     3061 ggctcatcaa cggagagtgc cggcagcgct tcaacgtttt gcgtgtcgca aacggtgtca
     3121 gaaatgttca gttcgcccag gcccgtgatc gccacgatat cgccagcttc cgccagatcg
     3181 gtttcgatac gttccagacc gaggtggccc agcactttac cgactttcgc gttacgggtt
     3241 ttgccttcgc tatcgatgat agtaacctgc tggttcggct tcactttacc acgcttgatg
     3301 cggccaatgc cgataacgcc aacatagctg ttgtaatcga gctgagaaat ctgcatctgg
     3361 aacggaccgt caaggtcaac gtccggcgca ggaacgtggt caacaatcgc ctgatacagc
     3421 ggggtcatgt cttccgccat atcttcgtgg tccagacccg cgataccgtt cagcgcagaa
     3481 gcgtaaacga tcgggaagtc cagctgctcg tcggtcgcgt cgaggttaac gaacagatcg
     3541 aatacctgat ccacaaccca atcaggacgc gcgccagggc ggtcaacttt gttgataaca
     3601 acaatcggct tcaggccgta agcaaacgct tttttggtta cgaagcgcgt ttgcggcatc
     3661 gggccgtcaa atgcgtcaac caccagcagc actgagtcta ccatggacat tacacgttca
     3721 acttcaccac cgaagtcggc gtgccccggg gtatcaacga tgttgatacg gtaatcattc
     3781 catttgatag cggtgttttt cgcgaggatg gtaatcccac gctctttctc caaatcgttg
     3841 gagtccatca cgcgctcttg ggtttcggca cgagagtcga acgtaccgga ttgttggagc
     3901 agcttgtcta ccagggtggt tttaccatgg tctacgtgcg cgatgatggc gatattacgc
     3961 aatttttcga tcacaacttt gcctcaggca ttagaaatag cgcgttattg tacacggatt
     4021 aatcgcacta caaaacagga tcacaaacat cctccgcaaa caagtattgc agagtccctt
     4081 tgtgatcgct ttcacggagc ataaaaaggg ttatccaaag gtcattgcac caatatggtg
     4141 cttaatgttt ccattgaagc actatattgg tgcaacattc acatcatggt gcagcccttt
     4201 tgcacgatgt taagcatgat aacgcctttt aggggcaatt taaaagttgg cacagatttc
     4261 gctttatctt ttttacggcg acacggccaa aataattgca gatttcgtta ccacgacgac
     4321 aatgaccaat ccaggagagt taaagtatgt ccgctgaaca cgtactgacg atgctgaacg
     4381 agcacgaagt gaagtttgtt gatttgcgct tcaccgatac caaaggtaaa gaacaacacg
     4441 tcactatccc tgctcatcag gtgaatgctg aattcttcga agaaggcaaa atgtttgacg
     4501 gctcctcgat tggcggctgg aaaggcatta acgaatccga catggtgctg atgccagacg
     4561 catccactgc agtgattgac ccgttcttcg ccgactccac cctgattatc cgttgcgaca
     4621 tccttgaacc tggcaccctg caaggctatg accgtgaccc gcgctctatt gcgaagcgcg
     4681 ccgaagatta cctgcgttcc actggcattg ccgacaccgt actgttcggg ccagaacctg
     4741 aattcttcct gttcgatgac atccgtttcg gatcatctat ctccggttcc cacgttgcta
     4801 tcgacgatat cgaaggcgca tggaactcct ccacccaata cgaaggtggt aacaaaggtc
     4861 accgtccggc agtgaaaggc ggttacttcc cggttccgcc ggtagactct gctcaggata
     4921 ttcgttctga aatgtgtctg gtgatggaac agatgggtct ggtggttgaa gcccatcacc
     4981 acgaagtagc gactgctggt cagaacgaag tggctacccg cttcaatacc atgaccaaaa
     5041 aagctgacga aattcagatc tacaaatatg tcgtgcataa cgtagcgcac cgcttcggta
     5101 aaaccgcgac ctttatgcca aaaccgatgt tcggtgataa cggctccggt atgcactgcc
     5161 acatgtctct gtctaaaaac ggcgttaacc tgttcgcagg cgacaaatac gcaggtctgt
     5221 ctgagcaggc gctgtactac attggcggcg taatcaaaca cgctaaagcg attaacgccc
     5281 tggcaaaccc gaccaccaac tcttataagc gtctggtccc gggctacgaa gcaccggtaa
     5341 tgctggctta ctctgcgcgt aaccgttctg cgtctatccg tattccggtg gtttcttctc
     5401 cgaaagcacg tcgtatcgaa gtacgtttcc cagatccggc agctaacccg tacctgtgct
     5461 ttgctgccct gctgatggcc ggtcttgatg gtatcaagaa caagatccat ccgggcgaag
     5521 ccatggacaa aaacctgtat gacctgccgc cagaagaagc gaaagagatc ccacaggttg
     5581 caggctctct ggaagaagca ctgaacgaac tggatctgga ccgcgagttc ctgaaagccg
     5641 gtggcgtgtt cacggatgaa gcgatcgatg cgtacatcgc tctgcgtcgc gaagaagatg
     5701 accgcgtgcg tatgactccg catccggtag agtttgagct gtactacagc gtctaatagt
     5761 tgaagttgta ctacccggcg cacaacgccg ggatttagtt gccgtggaaa ctttcagccc
     5821 atctctgcat gggctttttt ctccgtcaat tctctgatgc ttcgcgcttt ttatccgtaa
     5881 aaagctataa tgcactaaaa tggtgcaacc tgttcaggag actgctttat ggcaacaggc
     5941 acgcagcccg atgctgggca gatcctcaac tcgctgatta acagtatttt gttaattgat
     6001 gacaatctgg cgatccatta cgccaacccc gccgcgcaac aactgctcgc ccaaagctcc
     6061 cgcaaattgt ttggtacgcc gttaccggaa ctgttgagct acttctcatt aaatatcgag
     6121 ctgatgcaag aaagtctgga ggcggggcaa ggctttaccg ataacgaagt gacgctggtc
     6181 atcgatggtc gctcgcatat cctttctgtg acggcccagc gtatgccgga cggcatgatc
     6241 ctgctggaga tggcaccgat ggataaccag cgccgcttaa gccaggaaca actacaacat
     6301 gctcaacaag tagctgcccg tgatttagtg cgcggcctgg cacatgagat taaaaatccg
     6361 cttggcggtt tacgtggcgc ggcgcagtta ctcagcaaag cgttacctga cccatcacta
     6421 ctcgaatata ccaaagtgat tatcgaacag gcggaccggc tgcgaaattt ggtcgaccgt
     6481 ctgttggggc cgcagctgcc cggtacgcgc gttaccgaaa gtattcacaa agtggctgaa
     6541 cgcgtggtaa cgctggtgtc gatggaactg ccggacaacg tgcggttgat tcgtgattac
     6601 gatcccagcc tgccggaact ggcgcacgac ccggatcaaa ttgaacaggt cttgctgaat
     6661 attgtgcgca atgcgctaca ggcgctgggg ccggaaggcg gtgaaatcat tctgcgtacc
     6721 cgcaccgcgt ttcaactgac cttacacggc gagcgctacc ggctggcggc gcggattgat
     6781 gtggaagata acgggccggg cattccgcct catttgcagg atacgctgtt ttacccgatg
     6841 gtcagcggcc gcgaaggtgg caccgggctt ggcttatcca tcgcccgtaa tttgattgat
     6901 cagcattcag gcaaaattga atttaccagt tggccaggtc ataccgagtt ctcggtttac
     6961 ctgcctatca ggaaataaag gtgacgttta tgcaacgagg gatagtctgg gtagtcgatg
     7021 acgatagttc catccgttgg gtgcttgaac gtgcgctcgc tggagcgggt ttaacctgta
     7081 cgacatttga gaacggcgcg gaagtactgg aggcgctggc gagcaaaacg ccggatgtgc
     7141 tgctttcaga tatccgtatg ccgggaatgg acgggctggc gctgctcaag cagattaaac
     7201 agcgccatcc aatgcttccg gtcatcatta tgaccgcaca ttccgatctg gatgctgccg
     7261 tcagcgccta tcaacaaggg gcgtttgatt atctgcccaa accgtttgat atcgacgaag
     7321 cagtggcgct ggttgagcgc gctatcagtc attaccagga acagcagcag ccgcgtaata
     7381 ttcagcttaa cggcccaacg accgatatca tcggcgaagc gcaagccatg caggacgtgt
     7441 tccgtattat cggtcggctt tcgcgttctt ctattagcgt gctgattaac ggcgaatccg
     7501 gcaccggtaa agaactggtc gctcatgccc tgcatcgcca cagtccgcgc accaaagcgc
     7561 cgtttatcgc gctgaatatg gcagctatcc caaaagattt gatcgaatca gaactgtttg
     7621 gccacgagaa aggcgcgttt actggcgcga ataccattcg tcaggggcgt tttgaacagg
     7681 ccgatggcgg cacgttattt ctcgacgaaa ttggtgatat gccgctggat gtgcagacgc
     7741 gtttgctgcg cgtgctggca gacggtcagt tttatcgcgt tggcggctat gcgccggtga
     7801 aagtggatgt gcggattatc gctgccactc accagaatct tgaacagcgg gtgcaggaag
     7861 gtaagttccg tgaggatctg ttccaccgcc tgaacgttat ccgcgttcat ctgccgccgc
     7921 tgcgcgaacg tcgggaagat attccccgtc tggcgcgcca ttttttacag gttgccgcgc
     7981 gcgaactggg cgtagaagcg aagttgctgc atccggaaac cgaagctgct ctgacgcgtc
     8041 tggcgtggcc aggcaacgtg cgccagctgg aaaacacctg ccgctggcta acggtgatgg
     8101 ccgccgggca ggaagtgttg attcaggatt tgcccggcga actgtttgaa tcaacggttg
     8161 cggagagtac ttcgcaaatg caaccggaca gttgggcgac acttttagcg cagtgggcag
     8221 acagagcgct gcgttccggt catcaaaatc tgctttccga agcgcagcca gagctggagc
     8281 ggacgttact gacgaccgcg ttgcgacata cgcaggggca taaacaggaa gcggcgcggc
     8341 tactcggctg gggccgcaac actctgacgc gtaagttaaa agagctgggg atggagtgat
     8401 tcacggcttg tgtgtaaaga ttgattattg agcgcaaatt gctggtattt tgcgctttac
     8461 tgttccgata agttcagtat gatcttgccc ggaaaacggg gagagtcatt atgctggaat
     8521 caataattaa tctggtatcg agtggcgcag ttgacagcca cacaccgcaa actgccgttg
     8581 ctgccgtgct gtgtgccgcg atgattgggc tgtttagctg aagctcgttg tttatgccgg
     8641 atgcggagaa agtcgtaggc ctgataagac gcgaccagcg tcgcatcagg catcatgctc
     8701 agatgccaga tgcggcgtaa acgccttatc cggcctacaa aagcaataat ttcaacaggt
     8761 tttgttgccc agcgcatcag gcaatcttgt tctcagcaca ttgatggcgc tttcgttttc
     8821 tactttgtaa acgaagcgcc attcactctt taaatcaccc gtgagaactg ctgcatccgc
     8881 gctttctggc gcagataggt atcaaagcac atgcaaatgt tgcggatcag caagcgacct
     8941 ttcgccgtca cctgtattcc cttctcatcc acatccacca gcccatcttt tgctaacggg
     9001 gcgagcagct tgagatcttc ctcaaagtaa tcagcgaagt gcaaatccca ctgtttctca
     9061 agaggggcgt aatccagacg gaagttgcag atgagcgact taatcacatc gcggcgaata
     9121 cagtcatcac gcgttagcgc aataccacgc cacagcgcat tgccttgttc atacacttgc
     9181 tgatagtact gcttcaactc tttctggttc tgcgcgtagc agtcgccaat catgctgatg
     9241 gcggaaacgc ccatccccag cagatcggta tcgccctgag tggtgtagcc ctggaagtta
     9301 cgatgcagca cgccttcacg ctgggccacc gccagctcgt catcaggacg ggcaaagtga
     9361 tccataccga taaactgata gcccgattgc gtcaggaagg cgatggtttc ctgcaggata
     9421 tcgagttttt gctgcggact cggcaggtca gcatctttga ttttgcgctg agcagcaaaa
     9481 atggtcggca gatgcgcgta gttaaaaacg ctcagacgat cggggttcag ctccgccaca
     9541 cgtttcaggg taaaggcgaa actctccggc gtctgtttcg gcaggccgta aatcaggtcg
     9601 atgttggtgg aggtaaaacc aatctcacgc gcatggttaa gcagtgcaaa gatgaactct
     9661 tcatcctgct cgcggttaac cagacgttgc acttctttgt tgaagtcctg cacgcccatg
     9721 ctcaggcgat taaagccttc ggcgcgtaaa tgatcgagta catccagttc gatttcccgc
     9781 ggatcgactt cgatcgaaat ctccgcatcg gtattgaaat ggaagttttc gcgcagcagc
     9841 ttcatcagac ggctgatttg cgctttattc agatacgtcg gcgttccgcc gccccagtgc
     9901 aattggctga cgtgacgccc ggcgaacagc ggtgcacgat ggacgatttc ttgctccagc
     9961 gcgtccagat actgatcggc cttgtgctgc tggcgagtaa caatcttatt gcaaccgcag
    10021 aagtaacaaa gcttatggca gaacgggata tgtacgtaga gagataatgg acgctcagga
    10081 tagcgcgcca cggcttgtaa aaacgcctgt tcgccgaagt cttctgaaaa ctccagcgcg
    10141 gtcgggtacg aggtgtatcg tggcccggaa tagttatatt tctggatcag ggccagatcc
    10201 cagtcgattt gctgtacaga catgctcact ccttccgatg gcgtctctgg cggctacggc
    10261 gtaccggcgg atgcccgttt cgggtcatca gccggttgcg cagccacttt tgtcgccgcg
    10321 acaaccgctg tagtttaacg aataaccaca ccagataaca tataaccgga agggttatca
    10381 gcagaacggg aaggcccacg gcggcaatcc gttagttgcc ccgcagcaga cgcatcatgt
    10441 cttcttgctt ctcgtcttct tcctcttctt cgtcatcgtc ataagagagg ccgagtttct
    10501 gcatcaactc atcaatacga tccagtttag catccaccca ggattgctct tcggcgctca
    10561 gggtttcgcc tgcttccaga cgttccagca gcgcatccag acgctcatcc gtttccagta
    10621 attccaactc cgcctgcggt gaaagcatag gtttctcact cttcggtttg tgctgtttgg
    10681 tgactttttc agtcactccc aatggaatag gggttttact gccaatacgt ggatcttttg
    10741 gtgcgttctg gcctttgctg cctgacgtgg tgtttccgcc cgctgcgcgg ctgcccggcg
    10801 catgaccacg acgttttttc tgacgcttac ggtcacgagc ttcctgatcc agctcctcgc
    10861 gtgtttttcg acgcgctttt gcatgacctt tgctgcgtga gtttgaagat gatggtttca
    10921 taatggttcg tcttttagcc gttttattca gtatagattt gcggcggaat ctaacagaaa
    10981 gcaagcaaag aaaaaaggcg acagattact ctgtcgcctt ttttcctgac tcataaccct
    11041 taacgggtct tacaatcctt gcaggccgcc aacacaccct gtttttccat tagccggaac
    11101 aatccgtgtt gattccattt ccgtttaatt acgtctccgg acgtttgtct tcctgacaat
    11161 cctctgtctt cgcctcctgg cgctcctgac cctcatcctg agtcattgtt cctgttagcg
    11221 tcctcgctct atgggtgcta ctttacgcct ttgctttaaa caaacaatca accgccatca
    11281 gtatttgcac attttcagag ttttatataa aagcaattca ctgatttata tagcttattt
    11341 gacatgcatc gcagaatttt ctctgctatt tcgcataaag actatcggca atgtctcaca
    11401 aagcgtatgg cttttactta caacgctcac gccgaaaaat ccgcctttta ttatggttca
    11461 ggtataatcg ccacaaagct ttgactgacg gagacgaccg ctttgactaa tttgaattat
    11521 caacagacgc attttgtgat gagtgcgcct gatattcgcc acctaccttc cgataccgga
    11581 attgaagtgg cttttgcagg ccgttccaac gcaggtaaat ccagcgcgct gaacacgctg
    11641 actaaccaga aaagcctggc tcgtacctca aaaaccccag ggcgcaccca gcttatcaac
    11701 ctgtttgaag tggctgacgg caagcgtctg gttgacttgc ctgggtacgg ttatgcggaa
    11761 gtcccggaag agatgaagcg caaatggcag cgtgcgctcg gcgaatacct cgaaaaacgt
    11821 cagagcctgc aaggtctggt ggtgctaatg gatattcgcc atccgctgaa agatttggat
    11881 cagcagatga ttgagtgggc ggtagacagc aatatcgccg ttctggtgct gctgaccaaa
    11941 gcggacaaac tggcaagcgg cgcacgtaaa gcgctattga atatggtgcg tgaagctgta
    12001 ctggcgttta acggtgatgt gcaggttgaa acgttttctt cgttgaagaa acaaggcgta
    12061 gacaagctga ggcagaaact ggatacctgg tttagcgaga tgcagcctgt agaagaaacg
    12121 caggacggcg aataattttc ttgccttaat gcttgtgccg gatgtggcgt atccggcccg
    12181 taaattcaat agattacaat tctaccgttg gcctgataaa catagcgcat caggcattac
    12241 ggatcttttc tttcgcccaa taaaaaacgc cccagtcatt actgactggg gcggctaaaa
    12301 tattcagcca aatccgatta cgtgaagtaa aaggtctgaa agatagaaca tcttacctct
    12361 gtaccctacg cgactaactt tactcttttt tgttcagttc agaaagcact ttttgtaatt
    12421 ttttttcagt tgttgcatag gaatttctaa tagccatcac aaaacgtgac agcttatgtt
    12481 gcttacttac gaaaaaaggg catgttcagg cgaatcttag tgcgcctgat cccagttttc
    12541 gccactcccc acttccacca gcaacggcac atccagacgg gtacagtttt ccatcagttg
    12601 atgaatctgc ttcgcgacgg catcaacatc atctttatga acttcaaata ccagttcatc
    12661 gtgtacctgc atgatcatac gtacacgcgg ttgctcagcc tgtaaccacg catcaacggc
    12721 aatcatcgcc cgtttgataa tgtcggcggc ggttccctgc attggcgcgt taatggctgc
    12781 acgttcagcc gctgcacgac gagcaccatt gctggatttg atatccggca gatacagacg
    12841 gcgtccgtcc agcgtttcaa cgtagcccag ctctttcgcc tgagcacggg tgcgttccat
    12901 atactccagc acgccagggt agcgttcgaa gtaaaggtcc atgtacttct gcgcttcttt
    12961 acgtggaatg ttcaattgcc gcgccagacc gaaagcactc atgccataaa tcagaccaaa
    13021 gttgatcgct ttcgcgctac ggcgttgctc gctggtgacg gtttccagtg gcaaaccaaa
    13081 cacttctgcc gccgttgccc ggtggatatc ttttccttcc gcgaatgcgg tcagcaagcc
    13141 tttatcacgc gagagatgcg ccataatgcg cagttcaatc tgcgagtagt ccgctgagac
    13201 aatcacataa tcctctggcg caataaacgc ctggcggata cgacgacctt cttcgttacg
    13261 caccggaatg ttttgcaggt taggatcggt tgacgataaa cgtcccgttg cagttactgc
    13321 ctggtgataa gaggtatgca cacgcccggt tttcgggttg atcatcagcg gcagcttgtc
    13381 ggtgtaagtc gacttcagct tcgccagacc acgatactcc agaatcactt ttggcaacgg
    13441 atagtccagc gccagttctt ccagtacctc ttccgacgtt gacggcgcgc cacccggcgt
    13501 tttcttcagc ggtttaatac cctgtttttc aaacagaatg gtttgcaact gcttggtgga
    13561 agaaaggtta aattcttcac ctgcaatttc atgcgctttc ttttccagct cagccagacg
    13621 caaggtgagc tcttcagaat gattgtgcag cactttcgga tcgatcttca caccgttacg
    13681 ttcaatgcgt gaaagcaccg gcaccagcgg catttcgata ttctcgaaga cgttcaacgg
    13741 ccctttgtgt ttctgcagat ccggccacat tttcagatgc aactgcaagg tgacatctgc
    13801 atcttcggcg gcgtaacgtc cggcctcttc gagggcaatc tggttaaagg tcagttgatt
    13861 tttgccttta cccgcaatct cttcaaaagt gatggttttg tgcttcaacc aacgttccgc
    13921 gaggctgtcc atatcgtgac gcccggcaac gctattgaga atgtaggact ccagcatggt
    13981 atcaaacgca atcccacgca gttcaatgcc gtaattcgcc agaataccgc gatcgtattt
    14041 caggttttgc ccgaccttca gcgccttttc atcttccagc agcggtttta gcaactcgag
    14101 tgcacgctcg tgagagattt gatcgggcgc atcaagataa tcatgagcaa ccggaatata
    14161 tgccgctacg cctggctcga tagcaaaaga aagcccgacc aggttagcag agatgttatc
    14221 aaggctgtcg gtttcggtat caaatgcaaa taccggcgct ttttccagct tcgcaatcca
    14281 cgctttcagt gtttcttcat caaggatagt gacgtagttg tcataagaaa tcaccgttgc
    14341 cgtcacttct ggcgcttcgt ctgcaacact ggtttcctgc ggcttcgcgg ctgtttttgc
    14401 ccctttggcc tgtaaccatt tgcccgcttc gacatcagca gtccagcgtt tgaactcata
    14461 ctttttgaac agccccaaca actcttccgc tgccggttgc tgcacttcca gttgttcaca
    14521 ggtcagctcc agttcaacat cggttttaat cgtcgccaac tggtatgaga gataagcaac
    14581 ttctttgttt tgctcgagct tcgctgccat tgttttcgcg ccacggaagc tcaacccagc
    14641 aattttttct ggctcggcat acagcgtatc cagcccgcca agaccttgca gcaatgcctg
    14701 cgcggttttt tcaccgacgc ccggtacgcc aggaatgtta tcagaggagt cacccatcag
    14761 cgccaggaag tcgatgatca attctggcgg cacgccgtac ttattcacca cctcttccgg
    14821 tccgaggatg gtattcgtca tggtattgat aagcgtaata tttggcgtca ccagctgcgc
    14881 catatcttta tcgccagtgc taatcagcac cggacgcccg gctttttcgg cttcgcgcgc
    14941 cagagtaccg ataacgtcgt ccgcttctac gccagaaacc gccagcagcg gcagtcccat
    15001 cgctttaacc atcgcgtgca gaggttcgat ttgtgctcgc agatcgtccg gcattggcgg
    15061 gcgatgtgat ttgtaatgtt caaacagttc atcacgaaag gtttttccct tggcgtcaaa
    15121 gaccaccgct gcatgcgtcg gtttatattg catgatcaga ctgcgcagca tgttgaggac
    15181 accatacatc gcaccggtcg gctcgcctgc gctgttagtc agcgggggaa acgcgtgata
    15241 tgcgcgataa agataagatg aaccatctac aaggataagt ggattttggg ggatctgaac
    15301 cataatgtcc gtgcctgttt atcagattat gggtaaagga tgccacagac aggatgaaaa
    15361 catgagtttt tcaccgcttt tcgtgaaaat cttttgtaga tctgctggat cgctcgcaaa
    15421 tcgtcatgtg gataactttg tgcataaatt atttacatcg cttttaattt attatcatgc
    15481 tttttacagc atgatgtttg tctttgtttt tcatgcagtt aaaaataaat ccatccctaa
    15541 taattgagat aatgggattt tttctaatgt ggatagatat gaattatttt tctccttaag
    15601 gatcatccgt tatttgggtc attttctgct aaaatctgcg ccttgcgctt ctttagtgca
    15661 ctgtctatgg ctaacctttt gaataaattc attatgacga gaatactcgc tgcgataacc
    15721 cttttgctga gtatcgtatt gaccattttg gtcactattt tttgttccgt cccgatcatc
    15781 attgccggga ttgtaaaact attgctacct gtgccagtca tttggcgaaa ggtttcgcgt
    15841 ttttgtgatt ttatgatgta ttgctggtgt gaaggtctgg cggtattact gcatctgaac
    15901 ccacacctgc aatgggaagt tcacgggctg gaagggttaa gtaagaagaa ctggtatctg
    15961 cttatttgta accaccgtag ttgggcagat attgtcgtac tgtgcgtgct gtttcgtaag
    16021 cacattccga tgaataaata tttcctcaag caacagctgg cctgggtgcc atttcttggc
    16081 ctggcgtgct gggcgctgga tatgccgttt atgaagcgtt attcccgcgc ttatttatta
    16141 cgtcatccgg agcgacgtgg tcaagatgtt gaaaccactc ggcgttcttg tgaaaagttt
    16201 cgcctgcatc ccaccaccat tgtcaatttc gttgaaggct cccgtttcac gcaagaaaaa
    16261 catcagcaaa cccactccac ttttcaaaac ttgttgccac caaaggcggc aggcattgcg
    16321 atggcgctaa atgtactggg taaacaattc gataaactgc ttaatgttac gctgtgttat
    16381 ccggacaata accgtcagcc gttcttcgat atgttaagcg gtaaactgac gcggattgtc
    16441 gtacatgtgg atttacagcc tattgccgat gagttacatg gcgactacat caatgacaaa
    16501 agctttaagc gccattttca gcaatggctg aactcattgt ggcaggaaaa agaccggctt
    16561 ctgacgtctc tcatgtcatc acagcgtcag aataagtaag ttttactcgc ctctgaatga
    16621 gcagaggcga gtgagtattt taatgaacaa aacgtccggc acgagacata aattcttctt
    16681 cgctcatctc ctgtccgtta aaaacaactt tacccggtgt ataacgcagc tgcagcgagg
    16741 cggtattgtt gtcaatcgtg attaactgaa acatctgacc taatgtcatc atcccgctga
    16801 tttgtttatc agcctgcttt tgcgcttttt ccgcatccat tccttcagat aaattaagct
    16861 gttttgcggt ttctgtgacg acattaagcg gtaacttcac atcaaaattg agcgatttga
    16921 tatctttgtt tgtggatgat gaagatttgg ctgggtcggc aatactgata tccagattgg
    16981 catttagttc gccgagtgcg ttcttccatc ttaccggttg tttaatggtc ggctcacttt
    17041 tttgtaataa cggtaaatac tctttgaaca atgcagcatt cacttcttgc agagcaactt
    17101 catcgtttgc taactcaggg tgtgcagcaa gctgcttctg catcgcaatg ttatactgga
    17161 tgataaattg gcgcactgct gagggatcaa tagattcagc aatcaatgac cactcaccac
    17221 taccgagttg ctgattccct tttgttaact tatcaagttc gtaggtcaga ttgacattga
    17281 tataatcttt gtctttaacg cgatccagcc tggtttttgc aacgaaagca tcgatttggg
    17341 caacgtcttc cccgtgattg ataatgttaa tctgattaaa tttcgactcg ctttcaccaa
    17401 ccggaaattt cttttcttct aatcgcgcca gagaatcaat ggtaaaactt tttgccgtta
    17461 ctttgttcat tggtgataat tgcagaacaa tatcttcgac tttgccttca atatccaggt
    17521 tagacaatga actaccatca ccattaaaag taaactggcc ctcaccaaaa gaaaattttc
    17581 catactctgc aggggcaaca tctaatgtcg tagagaatga gccaccaaac gcgaatcgca
    17641 atgtcgcttc cacgggcgat ttatttttcg cggcgataaa tagcggttgt gtcagttcat
    17701 tattcactaa gttcactttt gccgctgcca gtgctgggat gacattacca tgcattaaca
    17761 ttgtgatggg caacggaccg tgctcaacat ccacatcaaa tacaactttc tggcctggct
    17821 tgatattgag atcgggtgca ccgttatcga atgtcatttg catttgaaaa cgggaattaa
    17881 aaaagccttt gtcaaagttt ttataactta acgtcatatc gtaggcatgt tcacctttct
    17941 ttttcgcatc gttaaaatct ttaataaact tttcgatacc aggctgaatt tgcgtacctg
    18001 tgtaccatgt gccaccaccc cagattacgg ctaacgcaac aataacacct gtagctgact
    18061 tacgtatcat ataaatattc cctgataaaa cagcagaact ttgaatatcc acttatgctg
    18121 aaataatgtc cagcggcagg atgcattatc agaatgtatt tatatttatt aaataataaa
    18181 aaaagcccgt gaatattcac gggctttatg taatttacat tgaattattt ttcctcggac
    18241 agatatttca ccgtatcagc atactgctga acaaaaacat ccatattgct ggtatccata
    18301 ccctgcggat tcagctgata tttaccgtta acaaacatcg ccggaacacc acgcaattgc
    18361 acatcagctg cggctttttc ctgctgagcg accagagatt tcaccacgaa gctgttccac
    18421 gccgcgtcgt actcttcacc tttaatacct gcattgataa atacatcgcg gatatcagat
    18481 gctgaacgaa tggtctgggt tttctgtacg ccttcaaaca gcggaacagt cactttgtct
    18541 tccacgccca gcgccatcgc cacagcccat gcctgagtca gatctttgcc caggtcacca
    18601 cccatgaagt tgacgtggta tttagtcatc ttcacgcctt ccggcagttt tttcttcaca
    18661 ttatcagaaa tatgcagaac ttcttcaaac tgatagcagt gcgggcagaa gaaagagaaa
    18721 aactccagca cttgcggcgc gccagctacc ggtttttcca gggtagtgta ctgtttacca
    18781 tcttcatact gcgccgccga tgcgctaaac gctaaaacta aaccagccag cgccagccaa
    18841 atctttttca tgatctactc tctccgatta atacataggt gttaattgca aagggggttc
    18901 ttgtagaact tttgcctgtt ctataaaagt cgccgtctgt cgtagccagt aatcttcccc
    18961 ggttaaccac gggaaatttt tcgggaacgc gggatcagcc caatgccgca ttagccaggc
    19021 aagataataa accaaacgca tggcgcgtaa aggttcaatc agtccgattt cagcggtgtc
    19081 gaactcgcta aattcttcat aagcttcaat aatagtttcc agttgcatcc gctgctgggc
    19141 tttatcgcca ttgagcaaca tccacaaatc ctgaatagct ggaccattac gtgcatcatc
    19201 cagatcaaca aacattggac catcgcgcca gagaatattc ccggcgtggc agtctccatg
    19261 tagccgcaga acggtgaaat cttcccgcca gtgtgctgta acggcggcga tcagctcatc
    19321 tgtcgctttc agaaatgccg ctttcaaccc ggaaggtatc agtgtagcgt cctcaaacag
    19381 cttgcgaggc tcaatgagat attcattcag accgatggtc gggcgatgaa taaaaagctg
    19441 tttgcgcccc gtctggtgca tacgccctaa ataacgccca accgcttcca tctgatcgat
    19501 attatcagct tcgaactggc gaccaccgac gcttggaaaa acagcgaaat aaaatccctg
    19561 atgattcaat aaagtctgac cgttaaaggc cacaggtgct gcgaccggaa cttcatcatt
    19621 taccagctgc aacgcaaatt gatgttcttc gaggatttga tcggctgtcc aacgttcagg
    19681 gcgataaaat ttgacgacaa aacgtcgacg ctcttcgtcc tgaaattgat agacacggtt
    19741 ttcatagctg ttaagcgggg taagaccgga atccacccgg atcccttgct caaacagagc
    19801 gtccatgatg gtatccgggt gtagtgtctg gaaagtaaaa gcgctgttgt tcatcccatc
    19861 atccggaaaa cattacgaat gatgaaggat atcatttcac gatgattttg gtggcgccgc
    19921 ttacaagctt ttactcttta attacgccgc gagcgcgtaa cagcgccgtt ttgaagtctt
    19981 cttcataatc tttctgcaaa cccgggatca ccgcatcttt cgcggaatca cgcattttca
    20041 ggtgatagat cagaatgtca tccgtcaaat ccgccagttc gccgtcaaaa cctgactctt
    20101 tcgccagttt ctgcaaaaat tgcaacaagt taaagtctgg ctctttttgc caggctggct
    20161 ggaggagttc aataacttca ttcagacgtt tacatttcat agtaatgctc cttactcttg
    20221 agacagacac gttagcaggg tcaatcccac aataaaagag gcgatatcgg tgaatctgat
    20281 gacgacgata acaggcgttg tgctggcagg cggtaaagcc agacgaatgg gcggcgtaga
    20341 taaaggattg ctggacttaa atggcaaacc gctgtggcaa catgtcgctg acgcgcttaa
    20401 gacgcagctc tctcacgtcg tggttaatgc taatcgtcat caggaaatct atcaggcagg
    20461 cggtctgaaa gtgattgaag attcactggc ggattaccca ggccctctgg caggaatgct
    20521 ttcagtaatg cagcaggaag ctggtgagtg gtttttgttt tgtccgtgcg atacgcctta
    20581 cattccccat gatttagccg cccggcttaa tcatcagcgc aaagatgcgc ctgtcgtgtg
    20641 ggtccatgac ggtgaacgcg atcacccgac tattgctctg gtaaaccgcg ctattgagcc
    20701 tttattactg gaatatctgc aagcaggaga acgccgggta atggcattta tgcgtctggc
    20761 tggcggtcat gcggttgatt tcagcgatca taaagatgca tttgttaacg tgaatacgcc
    20821 agaggagctt gcccgatggc aggaaaaacg atgataccgt tactcgcctt tgccgcgtgg
    20881 agtggtaccg gaaaaactac gcttttgaaa aaactgatcc cggcattatg cgccagaggg
    20941 atccgtccgg ggctgattaa gcatacgcac catgatatgg atgttgataa gccaggcaaa
    21001 gatagctatg agctgcgcaa ggctggcgcg gcacaaacca tcgttgccag ccagcaacga
    21061 tgggccttga tgacggaaac accagacgaa gaagagctgg atctacattt tctcgcaagt
    21121 cgaatggata cctcaaagct ggatttgatt ctggtcgaag ggtttaagca tgaagagatc
    21181 gcaaagattg tgctgtttcg cgatggagcc ggacatcgac ctgaagaatt agtgatagac
    21241 aggcatgtta ttgctgtagc cagtgatgtg ccgcttaatc ttgatgtcgc attactggat
    21301 attaatgatg ttgaggggct ggctgatttt gtggtggagt ggatgcaaaa gcagaacgga
    21361 taagcaagag taggctggat cagacgc
//
LOCUS       DAJTQY010000063        21278 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.063, whole genome shotgun sequence.
ACCESSION   DAJTQY010000063 DAJTQY010000000
VERSION     DAJTQY010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21278
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.063"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(<1..447)
                     /locus_tag="OO349_002531"
     CDS             complement(<1..447)
                     /locus_tag="OO349_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HCR5867561.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRIPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLV"
     gene            complement(502..1353)
                     /gene="manZ"
                     /locus_tag="OO349_002532"
     CDS             complement(502..1353)
                     /gene="manZ"
                     /locus_tag="OO349_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HCR5867562.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVST
                     ITGQDGQTRVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            complement(1366..2166)
                     /gene="manY"
                     /locus_tag="OO349_002533"
     CDS             complement(1366..2166)
                     /gene="manY"
                     /locus_tag="OO349_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HCR5867563.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            complement(2229..3200)
                     /gene="manX"
                     /locus_tag="OO349_002534"
     CDS             complement(2229..3200)
                     /gene="manX"
                     /locus_tag="OO349_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HCR5867564.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMVPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            3663..5213
                     /gene="yoaE"
                     /locus_tag="OO349_002535"
     CDS             3663..5213
                     /gene="yoaE"
                     /locus_tag="OO349_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HCR5867565.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPIPE
                     GAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLFPV
                     CRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGS
                     FVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGNGWLVKGGTDLHALQQA
                     LDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITILEANDYRVDLVRIVKE
                     QPAHDEDE"
     gene            complement(5217..6815)
                     /locus_tag="OO349_002536"
     CDS             complement(5217..6815)
                     /locus_tag="OO349_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCR5867566.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSKVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVIEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQSFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTH
                     YVMAETIRQHHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMSLR"
     gene            complement(6946..8310)
                     /gene="sdaA"
                     /locus_tag="OO349_002537"
     CDS             complement(6946..8310)
                     /gene="sdaA"
                     /locus_tag="OO349_002537"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCR5867567.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVKERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            complement(8494..9072)
                     /locus_tag="OO349_002538"
     CDS             complement(8494..9072)
                     /locus_tag="OO349_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HCR5867568.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINREPLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVNP"
     gene            complement(9076..10437)
                     /gene="pabB"
                     /locus_tag="OO349_002539"
     CDS             complement(9076..10437)
                     /gene="pabB"
                     /locus_tag="OO349_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HCR5867569.1"
                     /translation="MKTLSPTVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTHEQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            10511..10690
                     /locus_tag="OO349_002540"
     CDS             10511..10690
                     /locus_tag="OO349_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HCR5867570.1"
                     /translation="MFAGLPSLIHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGELIVARFEDEDE"
     gene            complement(10810..11169)
                     /locus_tag="OO349_002541"
     CDS             complement(10810..11169)
                     /locus_tag="OO349_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HCR5867571.1"
                     /translation="MQIKVIYSLIDNMVNFKDKNMPAVIDKALDFIGAMDVSAPTPSS
                     MNESTAKGIFKYLKELGVPASAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIK
                     NPEYFSTYMQEELKALV"
     gene            complement(11531..11875)
                     /locus_tag="OO349_002542"
     CDS             complement(11531..11875)
                     /locus_tag="OO349_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCR5867572.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            12007..13917
                     /locus_tag="OO349_002543"
     CDS             12007..13917
                     /locus_tag="OO349_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HCR5867573.1"
                     /translation="MTDDFAPDGQLAKAIPGLKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDYRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            13975..14670
                     /gene="tsaB"
                     /locus_tag="OO349_002544"
     CDS             13975..14670
                     /gene="tsaB"
                     /locus_tag="OO349_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HCR5867574.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLSAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            14710..15291
                     /gene="yeaY"
                     /locus_tag="OO349_002545"
     CDS             14710..15291
                     /gene="yeaY"
                     /locus_tag="OO349_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HCR5867575.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            15496..17181
                     /gene="fadD"
                     /locus_tag="OO349_002546"
     CDS             15496..17181
                     /gene="fadD"
                     /locus_tag="OO349_002546"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HCR5867576.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGIIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHALEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            17263..18378
                     /gene="rnd"
                     /locus_tag="OO349_002547"
     CDS             17263..18378
                     /gene="rnd"
                     /locus_tag="OO349_002547"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HCR5867577.1"
                     /translation="MITTDDALASLCEAVRVFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEDALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(18432..19397)
                     /gene="yeaX"
                     /locus_tag="OO349_002548"
     CDS             complement(18432..19397)
                     /gene="yeaX"
                     /locus_tag="OO349_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HCR5867578.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHIL
                     VQMSDGDNQYSNAYSLLSSPHNTSSYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGIPPFLSHMAELQHSDIDWQLHYCSRNPESCAFRAELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALIEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(19453..20070)
                     /gene="yeaW"
                     /locus_tag="OO349_002549"
                     /pseudo
     CDS             complement(19453..20070)
                     /gene="yeaW"
                     /locus_tag="OO349_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaW"
     gene            complement(20412..20978)
                     /locus_tag="OO349_002550"
     CDS             complement(20412..20978)
                     /locus_tag="OO349_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5867579.1"
                     /translation="MPPEIMRFKERLEEAMNGESSRAFAAKCGLSDGVIRNYLSGKTY
                     PSLDRLAQIAYATGRPIEWFIQSQSMPMVEHKNNQENKNSQPDRSTLKTKLIAIVELL
                     EDKELHSAIELFRKKGFEALMPEIFSSDDSIQADLQGISQQTLQTAKMLESLPTDTRK
                     KILSKYGIYEQEGLVAPSQEPQDVKKAV"
     gene            21190..>21278
                     /locus_tag="OO349_002551"
     CDS             21190..>21278
                     /locus_tag="OO349_002551"
                     /inference="COORDINATES: protein motif:HMM:NF025073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5867580.1"
                     /translation="MSAKNQDWHAEQIKAAVRMKGKTLSQLSR"
BASE COUNT         5269 a         5275 c         5466 g         5268 t
ORIGIN      
        1 tacgagaagc ttataaattt tttccagatc gtcgatattt cgaacgcgga ttaacagccg
       61 acgctgttct aattgcatca ccaacacgcc atcttccgac aagttcatcg ctttgattcg
      121 gctatattca atccagacat tggcgaagaa aaaacctttt tgtttaaaga tgatcttcgg
      181 aatgcggatc cagaatatat aaaaacccat cagagccagt gcacttaata accatgttgt
      241 tattagcgcg ccatgattcg tgacgttgtt atagataaga atgacaatca atccgacgaa
      301 gataacgcta tcgatgcgac cacgccggag caaaggaatt gccagcaggg tggggccgtt
      361 acggcggggc atgatgaact gatcgtagat cgcgaaggcc aggagtgcgg cgatgaaaag
      421 aatcagtacc aggtccgtga ttgtcattaa tcctccagat aaaaaaacgg ggccaaaagg
      481 ccccggtagt gtacaacagt cttacagtcc cagcaggccg caagcgtaac cagcgatacc
      541 gatgacgaag aagccaacga tgatccacag cgggttaact tttttgcgca gcagccacat
      601 acaagcaaat gtcagcagca gtggtacaag gcctggcatt aactggtcaa gaatagtttg
      661 cacggtggtg acacgggtct gtccatcctg cccggtgatg gtcgaaacca ccagcgggat
      721 gttgacatgt gtccacttgt taaccaatgc ccccatgaca aacaggccga ggatagacgc
      781 cccttccgtc agtttttgca ggaagccacc acccatatct ttaacgatat cgataccttt
      841 ggagtaacca tacgctacgc cgtagtaacg ggttgccaga cgcaccaggt taaacaggat
      901 gaagaacagc agcggaccta acaggctgcc gctcatcgcg ataccggcac ccagtgctgc
      961 aaataccgga cgtacggttc cccagaagat cgggtcgcct acaccagcca gcggccccat
     1021 caaaccgact ttgataccgt tgatagcacc gtcatcgatc tctgcgccat tagcacgctg
     1081 ttcttccagc gccagggtta cgccgagaat cggtgcagcc acgaacggct gggtgttaaa
     1141 gaactccagg tgacggcgaa tagcttgttt acgagcttcg ttgttctcag ggtagaggcg
     1201 acgaattgcc ggtaccatag agaagcagaa acccagtgcc tgcatacgtt cgaagttcca
     1261 tgaaccctgg aagaggttag aacgcaggaa tacgccacga atatcacttt gagtgagttt
     1321 tttctcggtg gtagtttgag ttgtatcaac catttcgctc acctgttagt ccagttcgtt
     1381 atcgagatcg ttgttaccag ctgcctgagc aggcgcaccg gctacgcggt tgtatttcgg
     1441 gctaagttgg atgtagagca ctgccataac agtaccaatc acacccagag caaccaggtt
     1501 aaagttggtg aatgctgcgg ttacgaagcc gaggtagaag aacggcatca ggtagccagc
     1561 acgcatcatg ttgataacca tcgcataacc aaccaccacg atcatgcccc cggcgatatt
     1621 cagaccattg gtcaccactt ccggaatcgc attcagcatg ttctgtactt cgctggtacc
     1681 aacagacagc gcaacgatga cggccggaat agccacacgc attgcttgca ggaacagaga
     1741 agaaacgtgg atccaggaaa tcgctgtcag gttgccgtta tcagcagcct tatccgcagc
     1801 gtgctggaaa gcaacggtaa tagtacgaac gatgatggtc agtacctggc ccgcagcggc
     1861 cagagggatt gccagtgcga tacctgcacc aatgctctga tgacctgcga taaccagaat
     1921 ggtagaaatg atagaagcca gagcggcgtc aggcgcaact gcagcaccga tgttcatcca
     1981 gcccagcgcg atcatttcca gcgtaccacc gataataata ccggttttca tatccccaag
     2041 aacgataccc accagggtac acgcgattag cggacggtga aactgaaatt catcgaggat
     2101 tgatcccata cctgcgatac aggctacgat aaataccagc acaatttgaa gagtggtaat
     2161 ctccattgta cttctcctgt ttacgtaaga cttaagtgtg aaaactgaat gataatcaac
     2221 acaatacgtt acttatcgat tttgctgatc agatccatca ttttcagttt cggatcggtg
     2281 gaaaccttac ggacttccag ctcaataccg cgtgcattca gttttttgaa cgcctcgata
     2341 tctttttcat caaccgaaac cgcgttattc acctgggttt taccctgacg gaatgccata
     2401 ccaccgacgt taacagaggt gattttcacg ccgccttcaa cgagacgctc tacatctgtt
     2461 gggttggtaa ataacagcat tacgcgttcg ccagcatatt tcgggttgtt gtagacgcga
     2521 atcattttgg caacatcaac tacgtgtgct gttacgcccg gaggtgcaac ctgggtgagc
     2581 agtgtcttac gaacggtatc cgcagccact tcatcactaa caacaataat acgggagaca
     2641 ttggtttctt tggtccagcg ggtggcgacc tgaccgtgaa tcagacggtc gtcgatacgc
     2701 gcaaggccaa taaccatgta gtcgtttggc accattggtt ttgccggagt tggagccgct
     2761 tttggtgctg cggcagcggg tgctggcgcg gctttttcaa ccggtttggc tttcagtgct
     2821 ttcacgcctt cacggcctgt ttctactgcc agcgcaacca gttcatcaaa gctcgggtca
     2881 tcatcacggg ccattaacgt ttccacgagc attggaatgt taacgcctgc aatgacttca
     2941 taatgctctt tgtcgacgac aatgcggctg gcagcattga acgggctgcc tccccatgta
     3001 tcaacgagaa acagcacgcc tttagtggtg tcgagttttg ccaactgagc gttgtatttt
     3061 tcaatcagcg tttcggcatt ttcacctgga acgaaatcga tccagccgac gttttcctgc
     3121 tcgcctaaca gcatttctgc cgttttaagc aactgctctg cagcccaacc atgtgtgcct
     3181 ataacaatag caatggtcac ttgctacctc ctttattatc gttaacacct caacgtgcca
     3241 gatgtatttt tgaatcgtcg tccacaatcg aatcgattca gataagggct acaaccaaaa
     3301 acccctgcgt ttcgcgattt attttagata tcgaaaaaat tattttatgt gatgaagatc
     3361 cgtaatttaa ctttcgatta tcagaaattt cgaaaggtaa aatatccttg tcacattcgt
     3421 ttgcaaagga aggtaaatct ttgccaaatc agaggcgtct ctgatatgtt taactcccgt
     3481 ttaacaacca tggagtatag ggcagtagcc cgcagtatgg atcgtcaccg acgtcatttc
     3541 agcatcaggc cttttaacgc ctgcctttct ggcactctat gccgcacctt tcgtttgcat
     3601 tttgtcgtta cgcctgcatt atttctggcg tcgaatagct attccttaag caggagcttg
     3661 tcatggaatt cttaatggac ccctcaattt gggcggggct actcacgctt gttgttctcg
     3721 aaattgtgct gggtatcgat aacctggtct tcatcgccat tcttgctgac aaactgccgc
     3781 caaaacaacg cgataaagcg cgtttgctgg ggttatcact ggcgctgatt atgcgtctgg
     3841 ggctgctgtc gctgatttca tggatggtca cgctgaccaa accgctattt accgtcatgg
     3901 atttctcctt ctccggacgc gatctgatta tgttgttcgg ggggatattc ttgctgttca
     3961 aagcaacaac cgaactgcat gaacggctgg aaaaccgcga tcatgattcc ggccacggta
     4021 aaggctacgc cagtttctgg gtggtcgtca cacagattgt catccttgac gccgtcttct
     4081 cgttggatgc ggtaattact gcagtaggga tggttaacca tctgccggtg atgatggcgg
     4141 cggtagtgat tgcgatggcg gttatgttgc tggcatcgaa accgctgacg cgattcgtta
     4201 accagcaccc cacggtggtg gtgctctgtc tgagcttcct gttaatgatt ggtctgagtc
     4261 tggtggcaga aggtttcggt ttccacattc cgaaaggtta cctgtatgcc gcgattggct
     4321 tctcgatcat tattgaagtg tttaaccaga ttgcgcgtcg caactttatt cgtcaccagt
     4381 cgactttgcc gctgcgagcg cgtactgccg atgccatcct gcgtttgatg ggcgggaaac
     4441 gtcaggccaa tgttcagcac gatgccgata acccgatgcc gatcccggaa ggtgcatttg
     4501 ccgaagaaga acggtacatg attaacggcg tactgacgct ggcgtcgcgt tctctgcgcg
     4561 ggatcatgac gccgcgcggt gaaataagct gggttgacgc taatctcggg gtcgatgaaa
     4621 tccgcgagca actgctctct tcaccgcaca gtctgttccc ggtatgtcgc ggtgaactgg
     4681 atgaaatcat cggtatcgta cgtgctaaag aactgctggt ggcgctggaa gagggcgttg
     4741 atgtggcggc gattgcttcg gcgtctccgg cgattatcgt cccggaaacc ctcgatccga
     4801 tcaacctgct gggcgtgctg cgtcgcgctc gcgggagctt tgttatcgtg accaacgagt
     4861 ttggtgtggt acaaggcttg gtcacgccgc tggatgtgct ggaagccatt gcgggtgaat
     4921 tcccggacgc tgacgaaacg ccggaaatca ttaccgatgg taacggctgg ctggtaaaag
     4981 gcggtacaga tttgcatgcc ttgcagcagg cgcttgatgt tgagcacctt gccgatgacg
     5041 atgatatcgc gacggtcgcg ggcctcgtga tctcggcaaa tggtcacatt ccccgtgtgg
     5101 gcgatgtgat tgatgtaggg ccactgcata tcaccatcct tgaagccaat gattatcgtg
     5161 ttgatctggt tcgcattgtt aaagagcaac cggcgcacga tgaagatgag taagcgttaa
     5221 cgtaacgaca taatgggcgt gatatgtcca ttatgccggg cgggcggcgg ttggctgccc
     5281 gccagccatt tgggaaaatc acgtagcggc atcggctgtg cgtacaaata cccttgcaaa
     5341 atatgcacgc catgacggcg caaatacttc gcctgttctt gcgtttccac gccctccgcc
     5401 accagttcaa tattcagtct ttgccccagc gcgatgatga tatcggtcac cgtcgagtta
     5461 accgcgtcag aacctatagc tgcggtaaat gacttatcaa ttttcagcac gtcaggacgt
     5521 aatgtttcaa gccaggaaaa cgagctgttg ccggtgccga agtcatcaat cgccagtttg
     5581 acgtttttac gatgcagctc gcgggcaatc cgataatcaa catccagtaa ggcatcgcgt
     5641 tcggtgattt cgaggatcag ttgctgaatc gggtgagcgc taaaccagta ctgattgaga
     5701 tcttttatca gcacaccacg gcgaaaatgg ctgggtgcga cgttaatgcc aacatgaaac
     5761 tgactactca tcgggaaaac atggtgctga cgaatggttt ctgccatcac atagtgggtc
     5821 agtggcacaa ttaaatgatg ttcttccgcg ataggaataa acacatccgg tgaaatccag
     5881 ccctgacgcg gattattcca gcgcagcaaa atctctacac caatacattg ctggctgcgc
     5941 gcattaagca aaggctgaca gaacaattcg aactcatgtt gcgccaggcc cagattaatt
     6001 tcgcgggaaa agctcatccg gtaagcggtc gccagccagg cgatatagcc caccagtaaa
     6061 cttagcagca ccgccagcgg taattgtgtt ggaagatagt gccatgccag cgccgtagcc
     6121 cccggaccat ttacgttaat ggtaaaagga aagctttgcg aagaaacctg atagttttca
     6181 ttgtcttcag gttgcggaag ggaatctacc agcccattac catagagcaa atgccgtttg
     6241 tccaccgtca ggctggcgct actgatttgc ggcagttgtg gctcaaggag cattgccgtc
     6301 agtaagtcga tgttaatcat ctctatgacc ccagcattgc tactgcccgc tgcaggcgtc
     6361 cattgaatca aaaccggact gcctttaatc agggcacggt cgatcgtcag gcgtaaaagt
     6421 ggttgcggtg caggaagttc agccagaatg tccacgacgg gcacattgcg ataaccaaaa
     6481 atgctggagc aataaagtgt gccgtcttgc accaggccaa tggatcgcac agtttggagt
     6541 tttgccgcct gtttacgtaa tggcaaatgg gcgacagagc agggcagacc aatcagcgga
     6601 agcaagactt tgctacctgc ctgtagggga agcagtactt tatctaattc ctctacagcg
     6661 tgattggcga attgtactac ccgttgttga tttaagttac gctgtgaaat aaatcgcacg
     6721 ctcagggtcg aagcgagcgt aacgagtgcg caaatcgtac aaacaatcat tcgattacgg
     6781 cgataggttt taatgatccg ttgtgctttt tgcatgcggg ccaccctgta agcgtatggc
     6841 gacaaacgcc tgacgcaaca gtggaagtgt agtggggaat attacagcag acgagaaagc
     6901 gggtataaat tcgcccatcc gttgcagatg ggcgagtaag aagtattagt cacactggac
     6961 tttgattgcc agaccaccgc gtgaggtttc gcggtatttg gcgttcatgt ccttaccggt
     7021 ttcgtacatc gtttcgatga ccttatccag cgagacgcgc ggtgcactgg tgcggcgcag
     7081 agccatccgt gcggcgttaa tcgccttcac agaggcaatg gcattacgct caatgcacgg
     7141 cacctgaacc tgccctgcaa ccgggtcgca ggttaaacca aggttgtgtt ccatgccaat
     7201 ttccgccgcc acgcaaacct gttccgggct accgcccagc agttctgcaa gacccgcagc
     7261 agccattgaa caggcaacac ccacttcgcc ctggcaacca acttccgcac cggaaataga
     7321 ggcgttcatt ttatacaatg caccaatcgc gcccgctgcc ataaagtaac gggtatagat
     7381 gtctgggctg accgattcaa taaagtggtc atagtaagcc agcactgccg gaacgatacc
     7441 gcaggcaccg ttggttggcg cagttaccac acgaccaccg gcggcgtttt cttcgttaac
     7501 tgccagcgca aacatgttta cccagtcaat gacattcatc ggatcgttag acagtttatc
     7561 gctggaaact aacatccggc gcagggcaga cgcacgacgt ggcacgcgca gcgggcctgg
     7621 cagtacacct tcggtgttca tcccgcgatc gatacatgcc tgcatggttt gccagacatg
     7681 cgcgaaatac tcgtcgatct ctttcttgct gtgcagcgcc agttcgttct gcatagcgag
     7741 accagacagc gaatagccgg tttcattaca gtacgcgagc agttcggtgg cagatttgaa
     7801 cggatacggc acgctcactt cgttggcagc atcctgacca aagtgttctt catcgacgat
     7861 aaaaccgccg ccgatggaat aataagtttt gctgtagacg acttcatcgc cgttataggc
     7921 gtggatttgc ataccgtttt catgcagcgg caggttgccg ttatgaaaac gcatcccgtt
     7981 gtcgcgcggg aaatccactt catgccgtcc ctgtgccagc agcagacgtt cgcgctcttt
     8041 tacgtcgcga ataaaaccgg gaatactgtc gatatccacg gtggcaggtt cgttacctgc
     8101 aagacccata ataatggcga tatcggtgtg gtggccttta cccgtcagcg acagtgaacc
     8161 ataaacgtcc acggcaacgc gagtaacgct atccagtaag cctttttcga ccagatcatc
     8221 gacgaactgt ttacccgcct tcataggccc tacggtatgg gaagatgagg gaccaatccc
     8281 caccttaaac atgtcgaata gactaatcac gataatactc ctgacaaggt gactggactt
     8341 ccagtaacga actaataaca gcgcatagtg taagagggaa cgcggcgact ggcttaacta
     8401 ttcacatgaa ttaaactaat caaaaaccgc gatttaacta atattgcgat gattgtctca
     8461 agtagtgttt tgtagatgta aagataagta tagtcagggg ttcacaccaa tttgcagcgc
     8521 cagctcacga attatgcctg cggtcattcc ccatacaaaa tactgttcgt accaggacag
     8581 ccatacccga tgtgaatcgc cacggcggta gatatctaaa gggtgataac gacccagatg
     8641 taatgcctgg gcgagcggca tttcaaacac cgccgagact tcatcttcac tggcgcgata
     8701 cggcagatcg ggcgggataa tgccgaccac tggggttact tgatagccag ttacgctatc
     8761 gacgggcggc agcacgccga taacttcaac ggcggaaggc ggtatagcga cctcttcttc
     8821 agcttcgcgc agcgcggcgg cgataactga tgcgtccgtg tcatcgactg cacctccagg
     8881 gaatgccact tgtccagcgt gtttacgcag atgaattgaa cgctgagtca gcaacaaccc
     8941 cggttgcggt cgacggacga tggggattaa cacggcagcc tgacgatgat ttaggggttc
     9001 ccggttaatt tgcgggcgca aaagttgaaa gcgagataaa aaatcatcaa gcgtcaggct
     9061 acggtattcc acgtcttact tctccagttg gcgtaatatc ttattaactt tatcaaaagt
     9121 ttcctgatat tccgcttctt cctggctatc ggcgacaatt ccaccgcccg cagagcagta
     9181 tatttgtccg ttaatggcag tcagcgtgcg gatagtaatg ctggtatcca tgttgccgca
     9241 aaagctcaaa tagccaatgc tgccgcacca ggcattacgt cgctgcggtt ccagttcgtc
     9301 gataatttcc atagcccgta ctttcggagc cccggttatt gagccaccag gaaaagctgc
     9361 gcgcagcaga tcgctggcgt gtaactgttc tggtagtcgc gccgttatag tgctgaccag
     9421 atgatgcacg gcagggaagg gttccaccac gaagagctct ggtactttta ccgaaccggc
     9481 tacggcaaca cgaccgatat cattacgcat taaatcgaca atcatcagat tttcggcacg
     9541 atctttcgct gagttcgcca gtttttctgc ttgtttgcta tcttcctgag gatcgggcag
     9601 gcgtggtagc gtgcctttaa tcgggcgggt ctggatttca ctattatcac aaagaataaa
     9661 ccgctctggc gaaaggctta aaattgcacc ctgttcaaga cgtaaaaaag cgctaaatgg
     9721 cgcgcggttg gcctgattaa gctgaaggaa tgcctgccat tcatcgccag aataggtcgc
     9781 atgaaaacgc tgggcgagat tcacctgata gcaatcaccg ctgtgcagat attcctgtac
     9841 ctggcgaaat ttttcgccgt actgctcgtg ggtcatattg gattgccagt cggaagtgag
     9901 cgtgaaatct tcctgcggcg agaattgctg gctttccagc caggcccgac gggcattgac
     9961 atcattatga ctcagcaaag aaactgtatg acgctggtgg tcgacaatga gcgcccaatc
    10021 gtagataccc actgccatat ccggcagaac gatatcttgt tccgcaattt ctggcagtga
    10081 ctcaaaacgg cggcccagat cgtagccaaa caaccccagt gcgccgccct gaaatggcaa
    10141 atcttcgtta tgcgttgggc gaatgtctgc gcgatccagc acctgctgga gcacctgtag
    10201 cgggtcatca gtggtcgttg tgcgtttttc gctttcacta acaacggttt ctttacctaa
    10261 agtggttaaa gtgcaaatcg gatcggcgac cacaatatca aagcggctat acggatgatc
    10321 ggcatagccg gagtgtaaaa gcatcgccca cggcaggtgg cttaagcggg agaaataaaa
    10381 ttcagcggcg tcctgacgcc agggtaaagt aatcacagtg ggagataacg tcttcatcag
    10441 tcctgactct actggctatg tgccgggtag catgaaataa ttagcgccaa caatttagca
    10501 ggagttaaca atgtttgcag gtttaccttc actcatccat gaacagcagc aaaaagctgt
    10561 cgagcggatc caggaactga tggcacaagg gatgagtagc ggccaggcaa tcgcgctggt
    10621 ggcagaagag ctgcgtgcca accactccgg cgagctaatc gtggcgcgtt ttgaggatga
    10681 agacgagtaa cacactaatc cgtatcgatt tcgttataga agccgatgat catttgattg
    10741 tgagaaaaag gacgcttttt atatgtgatt aaaagcgtcc tgacccattt atgctgatat
    10801 aaatttaact cagaccagtg cttttagttc ttcctgcata tatgttgaaa agtattcagg
    10861 atttttaatc acagagcgtt cacctgtctc catttttttt gcccatccaa ccattttttc
    10921 cgtgaaccca gggttccagc cttcctggtc agctcgcgcc gtaatatcag cggcactcgc
    10981 gggtactccc agttctttta aatatttaaa gattcccttc gccgtgcttt cattcatcga
    11041 acttggtgtt ggcgctgata catccatggc accaatgaaa tccagggctt tatctattac
    11101 agcaggcata ttcttatcct taaaattaac catgttatcg ataaggctat atatgacctt
    11161 aatttgcatc taatatcaaa gggattataa cgatatggca cgagttaacc tgttgacatt
    11221 caggtgtttt aaaggtagca gctcatggtg tgttatccag taagatggag aaaattaacg
    11281 gtcagcccgc acgctataac cagattgttc agctaagaaa gaaaaccaga gttgctaatc
    11341 aaagggcgaa cacaacggca tggttatgtt tatttatata gttagtttta tagataaaat
    11401 tgaaattata aatataaata atgaattagc agaagcatca acagtaatat cttattctct
    11461 ggtcgattta tgtaaaaccg accagtatta tcgataaatt atcgtctgac ttattttgct
    11521 tcgataaagc ttacaccgca gccacaatct taatttcaac tttatacttc gggttcatca
    11581 aacccgcttg taccgtgcag cgcaccggcg catgacccgc gacaacccag gcatcccacg
    11641 ctttattcat cgccgcgaag tcgtttttat cggccaggaa aatggtggca tccagaatgc
    11701 tcgatttatt gctgccctgt ttttccagca cggcgtcaat ctgtgccagc gtgttggcgg
    11761 tttgctcaaa ggcatcggcg tcgaggtttt ccggtacacc agtgtagtag agcgtgttgt
    11821 tgtggattac tacatcagac cagcgggctt cagcatcgat acgaacgata gtcatcattt
    11881 tgtcctcatt atacttccat tttcgtgctg caaagactgc cacaatgtga ggttgccgtc
    11941 accacttcaa ctggcgaaag cgccctcatc ctgacataat ccctgttcaa atcaacaggg
    12001 ggtagtgtga cggacgattt tgcaccagac ggtcagctgg cgaaagcgat accaggcctt
    12061 aagccgcgag aaccacagcg acagatggcg gtagccgtca cccaggcgat agaaaaaggc
    12121 cagccgctgg tggtggaagc aggaaccggt acgggcaaaa cctacgctta cctggctcct
    12181 gcgctgcggg cgaaaaagaa agtcattatc tcgaccggct caaaagcgtt gcaggatcag
    12241 ctctacagcc gcgatttgcc aacagtctca aaggcattga aatatacggg caacgtggcg
    12301 ctgctgaaag ggcgctcaaa ctacctctgc ctcgaacgtc tcgaacagca ggcgctggcg
    12361 gggggcgatc tgccggtaca aatcttaagc gatgtgatcc tgctgcgctc ctggtctaat
    12421 caaacagtcg atggtgatat cagcacctgc gtcagcgtgg cggaagattc acaggcgtgg
    12481 ccgctggtca ccagcaccaa cgacaactgt cttggcagcg actgcccgat gtataaagat
    12541 tgctttgtgg tcaaagcacg taaaaaagcg atggacgccg atgtggtggt ggtaaaccat
    12601 catctctttc tggcggatat ggtggttaaa gagagtggat ttggcgagtt gatccctgaa
    12661 gcggacgtca tgatcttcga cgaagcccac cagctaccgg acattgccag ccagtatttt
    12721 ggtcagtcac tctccagtcg acaactgctc gacctggcaa aagacatcac catcgcctac
    12781 cgcaccgaat taaaagacac ccagcagtta caaaagtgcg ctgatcgtct tgcccagagt
    12841 gcgcaggatt ttcgtctgca actcggtgag ccaggttatc gcggtaacct gcgtgagctg
    12901 ttagctaatc cgcaaattca gcgggcattt ttactgctcg atgacacctt ggaactttgt
    12961 tatgatgtgg cgaaactgtc actggggcgt tccgccttgc tggatgcggc atttgagcgc
    13021 gccacgttgt atcgcacacg gctgaagcgg ctaaaagaga tcaatcagcc gggctacagc
    13081 tactggtacg aatgcacttc gcgccatttt actctggctc tcacgccgct cagcgtggcg
    13141 gataaattca aagagttaat ggcgcaaaag cccggtagct ggatcttcac ctcagcaacg
    13201 ctgtcggtga acgacgatct gcatcatttc acctcgcggc ttgggataga acaggcggag
    13261 tcgttgctat tgccaagccc gtttgattac agccgccagg cgttactctg tgtgccgcgc
    13321 aatctgccgc aaaccaacca gccaggttct gctcgccagt tagcggcaat gctgcgaccg
    13381 atcatcgaag ctaacaacgg tcgttgtttt atgctttgta cctcgcacgc catgatgcgc
    13441 gatctggccg agcagttccg cgctaccatg acgcttcccg ttttgttgca gggggaaacc
    13501 agtaaaggcc aactgttgca gcagtttgtc agtgccggta atgcgcttct tgtggcaacc
    13561 agtagtttct gggaaggggt ggacgtgcgt ggcgatacat tgtcattggt aattatcgac
    13621 aaattgccgt ttacctcgcc ggatgatcca ctgttaaaag cgcgcatgga agattgtcgt
    13681 ttgcgcggtg gcgacccgtt cgatgaagtg caactaccag atgccgtcat tactctcaaa
    13741 cagggggtag gtcgactgat tcgcgacgcc gacgatcgtg gcgtgctggt gatttgtgac
    13801 tatcggctgg tgatgcgtcc ttacggcgcg acgtttctcg ccagtctgcc gcccgcgcca
    13861 cgcacccgtg acattgcccg tgcggttcgt ttccttgcga taccatcctc caggtaattt
    13921 gttacggagt atggtatgat gcgcgccttt ttattgatct aagcacgaag acccatgcga
    13981 attctggcta tcgataccgc gacagaggcc tgctctgtcg ccctgtggaa cgacggtact
    14041 gtcaacgctc attttgagct ttgccctcgt gaacatactc aacgaatctt accgatggtg
    14101 caggatatcc tgaccaccag cggaacttcc ctgactgata ttaacgctct ggcttacggg
    14161 cgcggccccg gtagctttac cggcgtgcgc attggtattg gcatcgcgca agggctggcg
    14221 cttggcgcgg aattaccgat gattggcgtc tccacgctaa tgacgatggc gcaaggggcg
    14281 tggcgcaaaa acggcgcaac acgcgtgctg tcagccattg acgcgcgaat gggcgaagtt
    14341 tactgggccg aatatcagcg tgatgaaaac ggtatctggc acggtgaaga aaccgaagcc
    14401 gtactcaaac ccgaactcgt tcatgaacga atgcaacagc tttccggcga atgggtgacg
    14461 gtagggacgg gctggcaagc ctggccggat ctcggtaaag agagcgggct ggttttgcgc
    14521 gatggcgaag tgttactccc cgctgcagaa gatatgctgc cgattgcttg tcagatgttt
    14581 gccgagggta aaacggtggc ggtggaacat gccgagccgg tttatttgcg taacaacgtc
    14641 gcatggaaga aacttccggg caaagaatga atcttaagag tatgccctga gaaaaaaagg
    14701 agtcgcatca tggcggttca aaagaatgtt atcaaaggca tactggcagg tacgtttgcg
    14761 ctaatgctga gcggttgtgt cactgtgccg gacgccatta aaggcagcag tcccacgccg
    14821 caacaagatt tagttcgggt gatgagtgcg ccgcagctgt acgttggtca ggaggcacgc
    14881 tttggtggca aagtggttgc ggtacaaaac cagcaaggga aaacccgcct ggaaattgct
    14941 accgtaccgc tggacagcgg agctaggccg acgctgggag aaccttctcg cggtcgcatt
    15001 tatgccgatg tgaacggttt tctggacccg gtggatttcc gtggacaact ggttacggta
    15061 gtcgggccaa tcaccggtgc ggttgacggc aaaatcggca atacgcccta taaatttatg
    15121 gtgatgcaag taacgggtta caaacgttgg catttaaccc agcaggtgat tatgccgcct
    15181 cagccgattg atccatggtt ttatggcggt cgtggctggc cctatggcta cggcggatgg
    15241 ggctggtata atcccggccc cgcgagagta caaacagttg taaccgaata attgcttgtt
    15301 tttaaagaaa aagaaacagc ggctggtccg ctgtttctgc attcttacgg taaagataaa
    15361 aataaatagt gacgcgcttc gcaacctttt cgttgggtaa ttatcaagct ggtatgatga
    15421 gttaatatta tgttaacagc gtgtatatca tttggggttg cgatgacgac gaacacgcat
    15481 tttagaggtg aagaattgaa gaaggtttgg cttaaccgtt atcccgcgga cgttccgacg
    15541 gagatcaacc ctgaccgtta tcaatctctg gtagatatgt ttgagcagtc ggtcgcgcgc
    15601 tacgccgatc agcctgcgtt tgtgaatatg ggggaggtga tgaccttccg caagctggaa
    15661 gaacgcagtc gcgcgtttgc cgcttatttg caacaagggt tggggctgaa gaaaggcgat
    15721 cgcgttgcgt tgatgatgcc taacttattg caatatccgg tggcgctatt tggcattttg
    15781 cgtgccggga taatcgtcgt aaacgttaac ccgttgtata ccccgcgtga gcttgagcat
    15841 cagcttaacg atagcggcgc atcggcgatt gttatcgtgt ctaactttgc ccacgctctg
    15901 gaaaaagtgg ttgataaaac cgccgttcag cacgtaattc tgacccgtat gggcgatcag
    15961 ctatctacgg caaaaggcac ggtagtcaat ttcgttgtta aatacatcaa gcgcttggtg
    16021 ccgaaatacc atctgccaga tgccatttca tttcgtagcg cactgcataa cggctaccgg
    16081 atgcagtacg tcaaacccga actggtgccg gaagatttag ccttcctgca atacaccggc
    16141 ggcaccacag gtgtggcgaa aggcgcgatg ctgactcacc gcaatatgct ggcgaacctg
    16201 gaacaggtca acgccaccta tgggccgttg ctgcatccag gtaaagaact ggtagtaacg
    16261 gcactgccgc tgtatcacat ttttgccctg accattaact gcctgctgtt tatcgaactg
    16321 ggtgggcaga acctgcttat cactaacccg cgtgatattc cagggttggt aaaagagtta
    16381 gcgaaatatc cgtttaccgc tatcactggc gttaacacct tgttcaatgc gttgctgaac
    16441 aataaagagt tccagcagct ggatttctcc agtctgcatc tttccgcagg cggtgggatg
    16501 cctgttcagc aagtggtggc agaacgctgg gtgaaactga ccggacagta tctgctggaa
    16561 ggttatggcc tgaccgagtg cgcgccgctg gtgagcgtta acccgtatga tattgattat
    16621 catagcggta gcatcggttt gccggtgcca tcgacggaag ccaaactggt ggatgacgac
    16681 gacaacgaag taccaccggg tcaaccgggt gagctttgtg tcaaaggacc gcaggtgatg
    16741 ctgggttact ggcagcgtcc ggatgctaca gatgagatca tcaaaaatgg ctggttacac
    16801 accggcgaca tcgcggtgat ggatgaagaa ggattcctgc gcattgtcga tcgtaaaaaa
    16861 gacatgattt tggtttccgg ttttaacgtc tatcccaacg agattgaaga tgtcgtcatg
    16921 cagcatcctg gcgtacagga agtcgcggct gttggcgtac cttccggctc cagtggtgaa
    16981 gcggtgaaaa tcttcgtagt gaaaaaagat ccatcgctta ccgaagagtc actggtgact
    17041 ttttgccgcc gtcagctcac gggatacaaa gtaccgaagc tggtggagtt tcgtgatgag
    17101 ttaccgaaat ctaacgtcgg aaaaattttg cgacgagaat tacgtgacga agcgcgcggc
    17161 aaagtggaca ataaagcctg agcgttaagt cagtcgtcag acgccggtta atccggcgtt
    17221 ttttttgacg cccactaaag agaaaacaat ttgaattacc aaatgattac cacggacgat
    17281 gcgctggctt ctttgtgtga agccgtccgt gtctttccgg cgatagccct ggatactgaa
    17341 tttgttcgta cgcgcactta ttacccgcag ctggggttga ttcaactttt cgatggcgag
    17401 catctggcgc taattgatcc tctcgggatc accgactggt caccgctgaa agcgatcctg
    17461 cgcgatccgt ccatcacaaa atttctccat gcaggcagtg aagatctgga agtgttcctc
    17521 aatgtctttg gcgaattacc acaacccttg attgacacgc aaatccttgc tgccttctgc
    17581 ggacgcccga tgtcatgggg tttcgcttcc atggtggaag agtattccgg cgttacgctg
    17641 gacaagagtg aatcgcgcac cgactggctg gccagaccgc tgaccgaacg tcagtgtgaa
    17701 tacgcagcgg cggatgtctg gtatctgtta ccgatcaccg ccaagcttat ggtagaaacg
    17761 gaggcctccg gctggctacc tgcggcgctg gatgaatgcc gcctgatgca aatgcgtcgt
    17821 caggaagtcg ttgcgccgga agatgcctgg cgtgatatca ccaatgcctg gcagttacgc
    17881 acacgccaac tggcctgtct gcaactgtta gccgactggc gactgcgcaa ggcgcgagag
    17941 cgtgatctgg cggtgaactt tgtcgtgcgt gaagagcatt tgtggtcggt cgcgcgttat
    18001 atgccgggaa gtttaggcga actggacagc ctgggtttat ccggtagcga aatccgcttt
    18061 cacggtaaaa cgctgctggc gctggtggaa aaagcgcaga cattgccgga agatgcctta
    18121 ccgcagccga tgcttaacct gatggacatg cctggttatc gtaaagcgtt taaagcgatt
    18181 aagtcgctga ttactgacgt gagcgaaacg cataagatca gcgccgaatt gctggcatcg
    18241 cgtcggcaaa tcaaccaact gctgaactgg cactggaaac tgaaaccgca gaacaatttg
    18301 ccggagctga tttccggctg gcgtggtgag ctgatggcgg aagcattaca caatttattg
    18361 caggaatatc cgcagtaaaa tcttccgaag ccggactggg cgcgctcagc gccacatccg
    18421 gctttggcaa actacaaatc caacaccagg cgtttaccct tcgcacgcga acaacagatc
    18481 aacatacttt gctggctggc acgctcttca tcgctaaaat actgatcccg atggtcagct
    18541 tcaccttcca gtattgccgt ttcgcaggtt ccgcataccc cttcacgaca taaacattcc
    18601 actttcgcgg ctttattatt ttcaataacc tgcaaaatag tcatctcttc cggcaccaca
    18661 aactcttttc cggaacgggc aagcaccagg gtaaatgcat cgccggtttt gtcttcgata
    18721 gcaaattgct caaagtgcag tgtatcggcg gcgatgtcca gacgcgcagc ttcacttctt
    18781 accgcttcaa ttagcgcctc ggggccacag gtataaacgt gtgtgccagg ttcgatatcc
    18841 gccaataatc gcgctaattc cagtcgtgtt ccggttgatg aatgatgcaa atggactttc
    18901 tcagcctgcg gatgctggac tagctcagca cgaaatgcgc aactttctgg atttcgcgag
    18961 cagtaatgta gctgccagtc gatgtcgctg tgttgcaact ctgccatgtg cgacaggaaa
    19021 ggggggatac caataccgcc cgcgataaac agatgctttc tggctgaggg aattagcgca
    19081 aacaggttat taggcgttga gatcgttaac cgatcgccca cttttacctg ctgatgcaaa
    19141 aagcgggaac cgccgcgcga gttttcttcc agtcgaacgg caatctgata actagaggtg
    19201 ttatgcggtg aactcagtag tgaatacgca ttgctgtact ggttatcacc atcgctcatc
    19261 tgcactagga tgtgacttcc tccggtgaac gcaggcagcg gtttgccatc ggttgccacc
    19321 agcgtgaagc gtttcacctg ttcggtaagg ggttcaacct ggctcacctg tacttcaaac
    19381 atttgatagt ctgacattgc tgtactccat caaaaatcag cccagcggcg gaaaataccg
    19441 ccgccgatgt cattagtcct taaacacctg cgccagcaga ttatggaaat gggcgatacc
    19501 atgttcggaa atgccactac cgctactgtc ggccatgatg cgcccctgac cacgatagcc
    19561 acgcgatttc agccctttct gtacgctttc aaccagacgt aaatcttccg gatggaacac
    19621 atcgcgatac cactcaatca gcgatttttg ctcgtcggtt aactcttcat tggtgaagta
    19681 aatatcgtag ttttgcaggg tagtttcaga atccaccggg aattcataaa tgaccgtcat
    19741 catccctttg atcggggtga cgttcagcat cgtgcacggc cacagccaga aaccgtggaa
    19801 tgccgcatcc gtaccctctt caaatttaaa cgactgttcg gacggtttgg cgaaaccgta
    19861 ttgcagcgtc cagttaccgt gcatggtgtg ccagtaacga tcaacctgta cggagtcgga
    19921 gaaacctaga tgcgccggac cacagtgata gcactcgaga tagttatcga caatgttctt
    19981 ccagttggca ggcgtgcggg tggtaaagcg ggccgctagt ttcagattgt ggacttccgg
    20041 gcaggcttcc agcactttcg cgcccagacc ggtgtattga ttcacttgaa gtacgaaaaa
    20101 aaaccgggag gacattggat tattcgggtt ctgatgggat tagatttggt ggggcttgca
    20161 agcctgtagc gcaaatttta gtcgttaatc aatgaaacgc gaaagatagt aaaaaattgc
    20221 ttttgtttca ttgaaaatac gaaaaacaaa aacactgcaa atcatttcaa taacagcttc
    20281 aaaaaacgtt caaaaccgat aacaaccagg ctgtaaccaa atgactcata tcacaaatca
    20341 gctcatgccg tttaggtatg ttacatgtgt gattatgtga ggtgaagtat gttttagctg
    20401 gttcatggtt gttatacggc tttttttaca tcttgtggtt cttgtgaagg agctacaaga
    20461 ccttcctgtt cataaatacc atactttgac aaaatctttt tacgagtatc tgttggtaat
    20521 gattcaagca ttttggctgt ttgtaaggtt tgctgcgaaa taccttgcaa atcagcctgg
    20581 atggaatcat cggaagaaaa aatctcaggc ataagagctt caaatccttt ctttctgaac
    20641 aactcaatcg cagaatgcag ttccttgtcc tctaacaatt cgacaattgc gatcagttta
    20701 gttttcaatg tgctgcggtc aggttgagaa tttttattct cttgattatt tttatgctca
    20761 accattggca tgctctgact ttggataaac cactctattg gacgccctgt tgcgtaggct
    20821 atttgtgcta atcgatctaa agacgggtat gtcttgccag acaggtagtt tctgataacc
    20881 ccatcagaaa gtccgcactt agctgcaaac gcccggcttg attctccatt cattgcctct
    20941 tcaaggcgtt ccttgaaacg cattatttcc gggggcataa aaatgcgctc tgcatttttg
    21001 ctttcttttt cacctgtcat gcccaatctc tctgttatat aaagaatttt acagaattct
    21061 ttgatttgca ctggttaaaa taaaaatgag catagctctt gaattgagca tttttctgcg
    21121 gtatcgtttt tcccgatagc ggtagccggt atcagttacc gaaaacgtta agagagtaag
    21181 gatcgcagaa tgtcagcaaa aaaccaagac tggcacgcag agcagataaa ggctgctgtt
    21241 cgtatgaagg ggaagacact atctcaatta tccagaga
//
LOCUS       DAJTQY010000064        21243 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.064, whole genome shotgun sequence.
ACCESSION   DAJTQY010000064 DAJTQY010000000
VERSION     DAJTQY010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21243
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.064"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            1..997
                     /locus_tag="OO349_002552"
                     /pseudo
     CDS             1..997
                     /locus_tag="OO349_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS150 family transposase"
     gene            1099..1251
                     /gene="hokA"
                     /locus_tag="OO349_002553"
     CDS             1099..1251
                     /gene="hokA"
                     /locus_tag="OO349_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HCR5867581.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     AYKLKE"
     gene            complement(1438..1650)
                     /gene="cspA"
                     /locus_tag="OO349_002554"
     CDS             complement(1438..1650)
                     /gene="cspA"
                     /locus_tag="OO349_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HCR5867582.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(1931..2221)
                     /locus_tag="OO349_002555"
                     /pseudo
     CDS             complement(1931..2221)
                     /locus_tag="OO349_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
     gene            2655..3365
                     /locus_tag="OO349_002556"
     CDS             2655..3365
                     /locus_tag="OO349_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HCR5867583.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(3415..4389)
                     /gene="ghrB"
                     /locus_tag="OO349_002557"
     CDS             complement(3415..4389)
                     /gene="ghrB"
                     /locus_tag="OO349_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HCR5867584.1"
                     /translation="MKPSVILYKALPDDLLQRLQAHFTVHQVANLSPQTVVQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRVHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(4493..5143)
                     /locus_tag="OO349_002558"
     CDS             complement(4493..5143)
                     /locus_tag="OO349_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HCR5867585.1"
                     /translation="MFILLAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSLVGA
                     GIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRSGDN
                     IILNMPNNVTFDSSSAPLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDLNMR
                     LSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSPL"
     gene            5306..7639
                     /gene="bisC"
                     /locus_tag="OO349_002559"
     CDS             5306..7639
                     /gene="bisC"
                     /locus_tag="OO349_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HCR5867586.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKNAEWAAEICGVGAAKIRELAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITE
                     GDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTTLAWLEKYNGPELTLTAFEPPASS"
     gene            complement(7608..8048)
                     /locus_tag="OO349_002560"
     CDS             complement(7608..8048)
                     /locus_tag="OO349_002560"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR5867587.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(8045..8608)
                     /gene="tag"
                     /locus_tag="OO349_002561"
     CDS             complement(8045..8608)
                     /gene="tag"
                     /locus_tag="OO349_002561"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HCR5867588.1"
                     /translation="MERCGWVSQGPLYIAYHDNEWGVPETDSKKLFEMICFEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFPDFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYLGNKP"
     gene            8766..9464
                     /locus_tag="OO349_002562"
     CDS             8766..9464
                     /locus_tag="OO349_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR5867589.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            9693..10901
                     /locus_tag="OO349_002563"
     CDS             9693..10901
                     /locus_tag="OO349_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5867590.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTVASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            11225..12916
                     /gene="eptB"
                     /locus_tag="OO349_002564"
     CDS             11225..12916
                     /gene="eptB"
                     /locus_tag="OO349_002564"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HCR5867591.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LVVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKVAAAN"
     gene            13008..13084
                     /locus_tag="OO349_002565"
     tRNA            13008..13084
                     /locus_tag="OO349_002565"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13042..13044,aa:Pro,seq:cgg)
     gene            13828..15435
                     /gene="dppA"
                     /locus_tag="OO349_002566"
     CDS             13828..15435
                     /gene="dppA"
                     /locus_tag="OO349_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HCR5867592.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTREMNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            15743..16762
                     /gene="dppB"
                     /locus_tag="OO349_002567"
     CDS             15743..16762
                     /gene="dppB"
                     /locus_tag="OO349_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HCR5867593.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHVQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            16772..17674
                     /gene="dppC"
                     /locus_tag="OO349_002568"
     CDS             16772..17674
                     /gene="dppC"
                     /locus_tag="OO349_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HCR5867594.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVAIVLF
                     IAIFANWIAPYNPADQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            17685..18668
                     /gene="dppD"
                     /locus_tag="OO349_002569"
     CDS             17685..18668
                     /gene="dppD"
                     /locus_tag="OO349_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HCR5867595.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            18665..19669
                     /gene="dppF"
                     /locus_tag="OO349_002570"
     CDS             18665..19669
                     /gene="dppF"
                     /locus_tag="OO349_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HCR5867596.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFVPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(19699..20970)
                     /locus_tag="OO349_002571"
     CDS             complement(19699..20970)
                     /locus_tag="OO349_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCR5867597.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPVTWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLIAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
BASE COUNT         5163 a         5210 c         5545 g         5325 t
ORIGIN      
        1 tatcagttga tccagaaaaa gcggcatcag cattggagct gtcaaaagac cgacgcattg
       61 aggatcttga aaggcaagtt cgatttcttg aaacgcggct tatgtatcta aaaaagctga
      121 aagccttagc tcatcccacg aaaaagtgaa agtactcaac gagctaaggc agttttatcc
      181 tcttgatgag cttctcaggg ctgcggagat accgcgcagt acgttttatt accatctaaa
      241 ggctctcagc aagcctgaca agtatgcgga cgttaaaaag cgtattggtg agatttatca
      301 cgagaataga ggccgatacg gataccgtag ggtaacgctg tctcttcatc gagaagggaa
      361 acagattaac cataaagctg ttcagcgcct gatgggaacc ctctcactta aagcagcgat
      421 taaggtcaag cgataccgct cttacagagg agaggtaggg caaaccgccc ctaatgttct
      481 ccaaagagat ttcaaggcta cgcggccaaa cgagaagtgg gttaccgatg ttactgaatt
      541 tgcagtcaat gggcgcaagc tgtatttgtc tccagtaata gatctcttca acaacgaagt
      601 tatttcttac agcctttcgg aaagaccagt gatgaacatg gttgagaata tgctcgatca
      661 ggcattcaaa aagcttaatc ctcacgagca tcctgttctg cactctgacc agggatggca
      721 gtatcgtatg agaagatatc aaaatatcct taaagaacat ggtattaaac aaagcatgtc
      781 cagaaaaggc aattgtctgg ataatgctgt ggtggagtgt ttctttggaa ccttaaagtc
      841 ggagtgtttt tatcttgatg agttcagtaa tataagcgaa ctgaaggatg ctgttacgga
      901 atatattgaa tactataaca gcagaagaat tagcctgaaa ttaaaaggtc tgactccaat
      961 tgaatatcgg aatcagacct atatgcctcg tgtttaactg tccaactttt tggggtcagt
     1021 acaaagtgag gctaccctat gcctgaacac cataaggtta gcctcttact cgttggaaat
     1081 caacacaggg ggctgggaat gccgcagaaa tatagattac tttctttaat agtgatttgt
     1141 ttcacgcttt tatttttcac ctggatgata agagattcac tgtgtgaatt gcatattaaa
     1201 caggagagtt atgagctggc ggcgttttta gcctacaaat tgaaagagta agagtcttcg
     1261 gcgggaaatt attcccgcct tacttacggc gttgcgcatt ctcattgcac ccatatttat
     1321 tcttcacaaa aataataata gattttatta cgcgatcgat tatttatttc ctgaaaaaaa
     1381 ataaaaaaat ccccgccaaa tggcagggat cttagattct gtgcttttaa gcagagatta
     1441 caggctggtt acgttaccag ctgccgggcc tttagcgccg ctttcgatgg tgaaggacac
     1501 tttctgacct tcgtccagag atttgtaacc atcgttctgg atagcagaga agtgtacgaa
     1561 cacatcttta gagccatcgt caggagtgat gaagccgaag cctttgtcag cgttgaacca
     1621 ttttacgata ccagtcattt taccggacat agtgtattac ctttaataat taagtgtgcc
     1681 tttcggcgat atggcgtgct ttacagattt tgaagcgtta aaggaatgtg cactacgagg
     1741 ggtatcaacg ataactcttg aagggacttg ccttactaca ctgctttaat ggtctgtacg
     1801 tcaaaccgtt gatgtgcatt aagccactca ttggcgggtg atgcaacaat tatttttcat
     1861 atttatgatt aatcggttgg aaaacggtcc tgtcatcagg accgtaaaca gcaataaagt
     1921 ggataaaagt ctatttcatc aactgcttac ttaatgccgg gttggcttga atcaaacgca
     1981 tcaattttag ttcggcactt gaaggcttca cgcgtctgga ttcccattcc tttaccatgg
     2041 cgactgatac gcccaaaacc cgggcgaaat catcgatttt taatcctgtc ccttttcata
     2101 actgctcaat ttcggtacag gacgttgttc tgtgcgtcag ggtaattttc tgcgtttcat
     2161 ctttaaaaac aatctgttcc aggctgctca acagctcatg cattggatct ttatattcca
     2221 ttgaaaactc ctcaaatcac actgcgggat cgtgaattgc atcgaagctc attaagaata
     2281 gtcgggaaaa gcagacacag cggggtgaag tcagggcatg ctctggctat gattcgattc
     2341 agcctgtcat ttagcgtgac ttatagccac ctaacgatct gataaatgcc gatatatctc
     2401 ttatcactcc tgttttgtaa agatttcgca aatctaacct tctgcgaagg cgtaaatctc
     2461 tggctacaaa aaaggcaacg gcactatcat tttttaacaa tgaaacaaca aattaacctt
     2521 gtttcatttt ctccatgatt ttaccaggaa gattacgtag aaagggtatc ctgcaagcct
     2581 gtcctggact atccttgtca cgtcagacac gcgtgtgtcg ttgtgcgctt tttttgggtg
     2641 aaaggagtaa gaaaatggcg acaggaaagt cctgctctcg ctggtttgcg cctcttgcgg
     2701 cgttattaat ggtagttagc ctgagtgggt gttttgataa agaaggcgat cagcgtaaag
     2761 cgtttatcga cttcctgcaa aatacagtga tgcgtagcgg tgaacgtcta ccaaccctga
     2821 ctgccgatca gaaaaaacag tttggtcctt ttgtctctga ttacgcgatt ctgtatggtt
     2881 attctcagca ggtgaatcag gcgatggatt ccggtctgcg cccggtcgta gacagcgtta
     2941 acgcaattcg cgtaccgcag gattacgtta cgcaaagcgg tccgctgcgt gaaatgaatg
     3001 gttcgctggg tgtgctggcg caacaactgc aaaatgcgaa gttgcaggcc gatgctgcgc
     3061 actctgcgct aaaacagagc gatgacctga aaccggtatt tgatcaggca ttcaccaaag
     3121 tggtaacgac gccagctgat gcattgcaac cgttgattcc ggcggcacaa actttcacgc
     3181 aacaactggt gatggttgga gactacattg ctcagcaagg tactcaggtg agctttgtcg
     3241 caaatggcat ccagttcccg acttcacagc aggcaagtga atataacaaa ctgattgcgc
     3301 cattaccggc acaacatcag gcgtttaatc aggcctggac tacagcagtt actgcaaccc
     3361 aataaagagt aaaagcccga gcgattatcg ggctttactt cacgcagtcg cagcttagtc
     3421 cgcgacgtga ggattcacac agttcttctc aacctttcct tgtaacgcat caatcaaatt
     3481 atccacggca caggcggcca tgccataacg cgtctcatgg gtggcagatc caatatgcgg
     3541 tactgcgacg acgttggcca ttgagagcaa cggcgaatct acggacagtg gctcttgttc
     3601 gaagacatcc agcccggcgg cgtgaatttc gcctttctgc aatgctgcga tcagtgcatt
     3661 ttcgtcaacc accgggccac gtccggcatt aatgaaaatg gcggaggatt tcattttggc
     3721 gaattgttct gcgccaaaca gatgatgcgt ctcatcagtt aacggcagga tcaggcaaac
     3781 gaaatctgac tcttgtaaca gagtatccaa atcgcagtag cgggcgttga agcgttcttc
     3841 tgcttcttta tggtggcggc gcgcgttata gaggatgggc atgttgaagc caaagtgcac
     3901 acgttgtgcc agcgccatgc cgatccgtcc catcccgaca atgcccagtg ttttatggtg
     3961 aacgtcagtg ccgtaccagt ccgggcctat gctcgcggtc cattcgcctg cttttacccg
     4021 ttctgctacc tccacaaccc gacgagcggt agacaacacc agcgccatca gcgtatcggc
     4081 gacggtttct gttaatacgg ttggcgtgtg catcagcaga atttttcggg cggtaagcgc
     4141 atcgacatca aaattgtcat agccgacgga gatcgttgat gtggcacgca gtttcggcat
     4201 tttttccagc aatgcggcat ctacattctc gtttgaaccc agtaaacctt cagcttctgc
     4261 aaaaattgct gcattttgaa cgacggtttg tgggctgagg tttgccacct ggtgaacggt
     4321 gaaatgcgct tgcaggcgtt gcagtaaatc atcaggtaag gctttgtaga ggataacgga
     4381 cggcttcatg cttctctcca ttcactgata aggcgatcac cttagcctga aatatgatga
     4441 aggaaaactg aagaatgcca ggtgatttct gcatcacctg gcatgaaagg gattacagcg
     4501 ggcttaaggt aatttctaca cggcggtttt gcgccttacc ttctgcggtg ctgttgctgg
     4561 cgattgggtt agccgggcca aggccctgag tacggatgcg gctggcgtcc acgccctggg
     4621 tgatcaacgc gctggcaacg gaatccgcac gctgctggga gagacgcatg ttcaggtcgt
     4681 gaccccccgt gctgtcggta taaccaatca cgttaaccgc cgttttcgga tactctttca
     4741 gcaccattgc cacgccggtc agggtgttag cgcctgccgg tttcagggga gcgctgctgc
     4801 tgtcgaaggt cacattgttc ggcatattga ggataatgtt atccccgctg cgggttacgc
     4861 taacaccagt gccgcgcatt ttgtcacgca gcttcgcttc ctgcacatcc atgtaataac
     4921 ccacgccgcc gcccagagct gcgcctgctg ctgcgccaat cagcgcgcct ttaccgcgat
     4981 ctttcttcga agaagagagc gcaccaatac ccgcgcccac gagagagccc agacctgcgc
     5041 cgatagcaga tttgcctgct tcgcgttcgc cggtgtaagg gttagttgtg cagccagata
     5101 ccgccagagc gccactcact acggcggcaa gtaagataaa cacgtttctt cattgttaat
     5161 ccttaataac ctttttattc tttgtcacgg gttccgtggc gggagattat gccgcgtgaa
     5221 catgaagatt attcctggga atactcggaa atttgtaagt aatatttaac tgctcaatac
     5281 atctaacctt tcaggagtct tcggtttggc caactcatcc tcacgatatt ccgttctgac
     5341 tgccgcccac tgggggccca tgctggttga aaccgacggc gaaaccgtgt ttagctcgcg
     5401 tggcgcgtta gccacaggaa tggaaaactc cttgcagagc gcggttcgcg accaggttca
     5461 cagcaatacg cgggtacgat ttccaatggt gcgcaaaggc tttctcgcgt caccggaaaa
     5521 cccgcaaggc attcgtgggc aggatgaatt tgttcgcgtg agttgggatg aggcgctgga
     5581 tcttattcac caacaacata aacgcattcg tgaggcttat ggtccggcat cgatttttgc
     5641 tggttcctac ggctggcgtt caaacggcgt gctgcataag gcctcgacat tattacaacg
     5701 ctatatggcg ctggcaggcg gttataccgg gcatctgggg gattattcga ccggtgcggc
     5761 acaggcgatc atgccgtatg tcgtaggtgg cagcgaagtt tatcaacagc aaaccagttg
     5821 gccgctggtg ctggaacata gcgatgtcgt ggtgttgtgg agtgctaacc cactcaatac
     5881 gctgaaaatt gcgtggaatg catccgatga gcaggggctt tcttactttt ccgcactgcg
     5941 tgacagtggg aaaaagctga tctgcattga tccaatgcga tcggaaaccg tcgatttctt
     6001 tggcgataaa atggagtggg tggcaccgca catgggcacc gatgttgcgc tgatgctggg
     6061 gatcgcccat acgctggtgg aaaatggttg gcacgacgaa gcgtttctgg cgcgttgcac
     6121 cacaggttat gccgtcttcg cctcttattt gctgggcgag agtgacggaa tagcgaaaaa
     6181 cgccgaatgg gcggcagaga tttgtggtgt tggcgcagcg aaaatccgcg agctggcggc
     6241 tattttccac caaaatacca ccatgctgat ggcaggctgg ggaatgcagc gccaacagtt
     6301 tggtgagcaa aaacactgga tgatcgtcac gctggcagca atgttggggc aaatcggcac
     6361 acccggcggc ggttttggtc tttcttacca ttttgccaat ggtggtaacc ccacgcggcg
     6421 ttctgcggtg ctctcttcca tgcagggcag cttgccgggt ggctgcgatg cggtggataa
     6481 aatccctgtt gcccgcattg ttgaagcact ggaaaaccct ggtggcgcat atcaacacaa
     6541 cggtatgaac cgacatttcc cggatattcg ttttatctgg tgggcgggcg gtgccaactt
     6601 tactcatcat caggatacca atcgtctgat ccgtgcctgg caaaaaccgg agctggtggt
     6661 gatctctgaa tgcttctgga cggcggcggc gaaacacgcg gatattgttc tgcctgcgac
     6721 tacctctttt gagcgtaatg atctcaccat gaccggtgat tacagtaatc agcatctggt
     6781 gccgatgaag caagtggtgc cgccacgcta tgaagcgcgt aatgattttg atgtttttgc
     6841 cgagttgagt gaacgttggg agaagggcgg ctatgcgcgg tttacggaag gaaaaagtca
     6901 gctgcaatgg ctggaaacgt tttataacgt tgcccggcag cgcggggcaa gccagcaggt
     6961 tgaattgccg ccatttgctg agttctggca agccaaccag ttaattgaga tgccggaaaa
     7021 cccggacagc gagcggttta ttcgcttcgc tgatttttgc cgcgatccgc tggcgcatcc
     7081 gttaaaaacc gccagcggca agattgaaat cttctcacag cgtattgccg attacggtta
     7141 cccggattgc cctgggcatc caatgtggct ggagccggac gaatggcagg gcaatgccga
     7201 accagaacag ttgcaggtac tttctgccca tccggcgcac cgcctgcaca gccagctgaa
     7261 ttacagttct ctgcgcgaat tgtacgcggt agcaaaccgt gagcctgtca ccattcatcc
     7321 tgacgatgct caggcgcgcg gcataaccga aggggatatg gtgcgggtgt ggaactcccg
     7381 tgggcagatc cttgccggag cggtcattag cgagggaatt aaacctggcg tgatttgcat
     7441 tcacgaaggg gcatggccgg atctggattt aaccgctgac ggtatttgta aaaacggcgc
     7501 agtgaacgtg ctgaccaaag atctccccag ctcgcggctg gggaatggct gtgcgggtaa
     7561 tacgacgctg gcatggctgg aaaaatacaa cggtccggaa ctgacactta cagcgtttga
     7621 accaccggcc agctcataat ccaggtgggt agttgggttt catcctgcca tgcgcaatcg
     7681 acaatgtgaa aaccctgcgc acggtaaaaa tctatcgccg gttgattttt ttgataaacc
     7741 tccagcatca gatggggata gcgctgctgc acatactgca tcagcgcctt accaataccg
     7801 cgtctgacgg ccttcggtgc gacaaacatt gctgccagaa atcggccttc cataatgctg
     7861 acaaaaccga gaagcttacc gtcctcttcc cagacccagt tttgcgcgtt ggcaagatag
     7921 gcatcccgca ccagcggaat gcagtcacgc cagtaattcg cttttataaa gggatgcccc
     7981 caggttgtac tttccagcca cagttcgagg atcgcgggga gttctgaacg ttgcgcttcc
     8041 cgaatcatgg tttatttccc agatagcaac agcagccaac cacatgatca ttcaccagcc
     8101 cacatgcctg cataaaggag taacagattg tggtgccgac aaacttaaaa ccacgttttt
     8161 tcagtgcctt agatagggcg tcggaggcgg acgtagatgt gggaatttcg ctcaacgttg
     8221 tggcttgtgt cacctgtggc tgatgattta caaacgacca gacaaagtcg ggaaacggtt
     8281 cgccgttctg ttccatttgc aggtacgccc gcgcattacc aataattgcc tgaattttcc
     8341 ctcgatggcg gataatcccg gcgtcctgta ccagtctttc gacatcctct tcctgcattg
     8401 ctgcgacctt caccggatcg aactgatgaa agcaggcgcg atagttttcg cgttttttga
     8461 ggacggtgat ccacgataac ccagcctgct gcccttcaaa gcagatcatt tcgaacagtt
     8521 ttttactgtc agtttcaggc acgccccact cattatcatg gtaggcaata taaagcgggc
     8581 cctgactcac ccagccgcaa cgttccatac tttccctcgc tatgtacccg atcgtaaaaa
     8641 tttcagccgc ttttcttgac gcatcggtga aaaagtcaat atttaataca gggctactat
     8701 gcccgatatc cttcatacaa tgacaataat attgccgaat tcggctcttc cctggagtcg
     8761 tgttaatgat cataaaaaaa agcggtggtc gctggcagct aagcctgctg gcgagcgtgg
     8821 taatcagtgc cttttttctc aacacagctt acgcctggca acaagaatat atcgttgata
     8881 cgcaacccgg acattccaca gagcgttaca cctgggatag tgatcatcaa cctgattaca
     8941 acgatatttt gtcgcaacgt attcaaagta gccaaagggc gctgggactg gaagtcaatc
     9001 tggcggaaga aactcctgtg gatgtgacca gcagtatgag tatgggctgg aattttcctt
     9061 tatatgaaca ggttacaacc ggcccggtcg cggcattaca ttacgatggc acaaccacct
     9121 cgatgtataa cgagtttggc gacagtacta ccacgctgac cgatccgtta tggcatgcca
     9181 gcgtgagtag cttaggctgg cgtgttgact cccggcttgg cgatctccga ccctgggcgc
     9241 aaatcagcta taaccagcaa tttggcgaga atatctggaa ggcgcaatca ggcctgagcc
     9301 ggatgacggc gacaaaccag aacggcaact ggctggatgt caccgtaggc gctgatatgt
     9361 tgctcaatca aaatattgcc gcctatgctg cgctaactca ggcagaaaat accactaata
     9421 atagcgacta tctgtatacg atgggggtta gcgccagatt ttaacgtaac agtcacaatt
     9481 gaaaccattg aataacaaca gttgtggcga tagtgggtgc taacttacca aataataaat
     9541 ttggtgaata attgtcgcgt cattcattcc tgaactaagg catttcattc cgttctgatg
     9601 gcatttcatg ccgtttttcc ccaagcataa aatgcacttc gttatggttg tcggcagaga
     9661 tttttccttt ttattactgc aggaatactg ccatgacacc ttcaaattat cagcgtaccc
     9721 gctggctgac actcatcggt actatcatta cccagtttgc gctggggtcg gtttatacct
     9781 ggagcctgtt taatggcgcg ctttctgcca agctggatgc gccggtaagc caggtcgctt
     9841 tctctttcgg cttgttaagt ctggggctgg caatttcgtc ttctgttgcg ggcaaattgc
     9901 aggaacgttt tggcgttaaa cgcgtcaccg tggcttccgg cattttgctg ggattaggct
     9961 tcttcctgac cgcgcattcc aacaacctga tgatgctgtg gttaagcgcc ggtgtgctgg
    10021 tcggtctggc ggatggcgcg ggttacctgc tgacgctctc taactgcgtg aagtggttcc
    10081 cggagcgtaa aggtctgatc tccgcgttcg ctatcggttc ttatggtctg ggtagcctgg
    10141 gtttcaaatt tatcgacacg cagctgctcg aaacggtggg tctggaaaaa acctttgtga
    10201 tttggggagc gattgcgctg gtgatgattg ttttcggcgc aacgttaatg aaagacgcac
    10261 caaaacagga agtgaaaacc agcaatggtg tggtggagaa agattacacg ctggcagagt
    10321 cgatgcgtaa accgcagtac tggatgttag cggtaatgtt cctgactgcc tgcatgagcg
    10381 gcctgtacgt gattggggta gcgaaagata tcgcccaaag tctggcacac cttgatgtgg
    10441 tttccgcagc caatgcagta acggttattt ccatcgctaa cctttcaggt cgtctggtgc
    10501 tgggcattct gtctgacaaa attgcccgta tccgtgttat caccattggt caggtgattt
    10561 cgctggtggg tatggcggcc ctgctgtttg caccattaaa tgcagtgacg ttctttgcag
    10621 cgattgcctg cgtggcattt aactttggcg gcactattac cgtctttcca tcactggtca
    10681 gtgagttctt cggcctcaat aacctggcga aaaactacgg tgtgatttac ctcggtttcg
    10741 gtatcggtag catttttggt tcgattatcg cctcactgtt tggcggtttc tatgtgacct
    10801 tctacgtgat tttcgccctg ctgattctgt cattggcgct ttctacgacg attcgtcagc
    10861 cagagcagaa aatgttgcgt gaggcgcatg gctcccttta atgctcagcc gcgagctact
    10921 gggcttcagc ttccgtgtgc atcaaacgca tcaacgaaat gtataaacaa tgactcgtaa
    10981 aatttgggcc agttaacagg ccctttttac tgctttttgt aacgctaaag tctcttttca
    11041 aacttgcatt tttgtaaatt tgtgcttcat gcacactctt tccccacact ttttcgcttt
    11101 gctgtggtct acttattcgc gcgtgtagat tttacttatc tgactacctc cgcacttttc
    11161 ccctgccggg cctgaaaagc cgctaagcag ggtgttatca cctgtttgtc cagggtttgt
    11221 ttgcatgaga tacatcaaat cgattacaca gcagaagctg agctttttgc ttgcaatcta
    11281 tattggcctt tttatgaatg gcgcggtttt ttatcgccgc ttcggcagct atgcgcacga
    11341 ttttaccgtc tggaaaggcg tttctgctgt tgttgaactg gccgccaccg tactggtgac
    11401 cttcttttta ctacgtcttc tatcgctgtt tggccgccgc agctggcgta ttctggcgtc
    11461 gctggtggtg ctcttttccg caggtgccag ctattacatg accttcctta atgtggtcat
    11521 tggttatggc atcatcgctt ccgtcatgac caccgatatc gacctgtcaa aagaagttgt
    11581 tggtctgaac tttattctct ggttaatcgc cgttagtgca ttgcctctta tccttatctg
    11641 gaataaccgc tgtcgctaca ccttgctccg acaactgcga accccagggc agcgtattcg
    11701 cagcctggtg gtcgtcgtac tggcgggtat tatggtttgg gcaccgattc gtttgctgga
    11761 tatccagcag aagaaagtgg agagggcgac cggcgttgat ttgccgagtt atggcggtgt
    11821 cgtagcgaac tcttatctgc catcaaactg gctttctgcg ttggggctgt atgcctgggc
    11881 gcgggtcgat gaatcttccg ataataattc attgcttaat ccggcgaaga agtttacata
    11941 ccaggctcca caaaacgttg atgatactta tgtcgtgttt atcatcggtg aaaccacgcg
    12001 ttgggaccat atgggtattt tcggctatga gcgtaatacc acgccgaaac tggcccagga
    12061 gaaaaatctg gcggcgttcc gtggttactc ctgtgatacc gcaaccaaac tctcactgcg
    12121 ttgcatgttt gtacgtcagg ggggcgcgga agataatccg cagcgcacat taaaagaaca
    12181 gaacattttc gcggttctga agcagttagg attcagttct gacctctacg ctatgcagag
    12241 cgaaatgtgg ttctacagca acacgatggc agacaacatt gcttatcgtg agcagattgg
    12301 tgcggagcca cgtaatcgtg gcaagccggt agatgatatg ttgctggtag acgaaatgca
    12361 gcaatcgcta gggcgcaacc cggatggtaa gcatctgatc attctgcata ccaaaggttc
    12421 gcattttaac tacacccagc gttatccgcg tagcttcgcg cagtggaagc cggaatgtat
    12481 tggtgttgat agcggctgta ccaaagcgca gatgatcaac tcctatgaca actcggtgac
    12541 ctatgtggat cactttatct ccagcgtgat tgatcaggtt cgcgataaga aagcgattgt
    12601 gttctacgca gctgaccacg gtgagtcaat taatgaacgc gagcacctgc acggcacgcc
    12661 gcgtgaactg gcaccgccgg agcagttccg cgtaccgatg atggtctgga tgtcagataa
    12721 atatctggaa aatccggcca atgcgcaggc gtttgcgcag ctgaaaaaag aagccgacat
    12781 gaaagtgcca cgccgtcacg tagagctgta cgacaccatc atgggttgtc ttggctatac
    12841 ttcaccggat ggtggaatta acgaaaacaa caactggtgt cacatcccgc agacaaaagt
    12901 ggcagcggct aactaagcgg cctgctgact ttctcgccga tcaaaaggca ttttgctatt
    12961 aagggattga cgagggcgta tctgcgcagt aagatgcgcc ccgcattcgg tgattggcgc
    13021 agcctggtag cgcacttcgt tcgggacgaa ggggtcggag gttcgaatcc tctatcaccg
    13081 accaaattcg aaaagcctgc tcaacgagca ggtttttttg catttgtagc tcatgaggat
    13141 gagaacctcc gggggcaggg aggctcgact cgacgtaggc ctgataagac gcgccagcgt
    13201 cgcatcaggc aacggcaccg cacttattgt cggatgcggc gcgaccgcct tattcgacca
    13261 acacaccaca cattttcctg ccaactttgc gccccatatc tcactatcca ttaacggatt
    13321 tgtgacagaa accattgtct ttttttatat ttgcttaaat cttttttgcg ttacttatgg
    13381 ctggcgctgg cattttccgc tgtgcgtttt agcgttcatg gtattaatcg ctcagatatc
    13441 caccttttcc tcagattttt ttcattaaat gcataaaact taatcaccaa atgttgctaa
    13501 atattaagct gattgttctg gttatgttgg agtagcacaa atttctctgc tgcaaatagc
    13561 tgtttgaagt ttttttaatc tttgtttgtg gattctcacc gttggtgtaa atcccggttg
    13621 gctgtattga cgttttcaca ttctgttgac agattgtagg tcacgagggg cattttatgg
    13681 aggatccgca ctgttacact gatgttaatt agtacggcat ccccacctca taacgttgac
    13741 ccgaccgggc aaaaaacaaa aaaggtcagg cagcgacaac ccactgcaaa gggttaaaac
    13801 aacaaacatc acaattggag cagaataatg cgtatttcct tgaaaaagtc agggatgctg
    13861 aagcttggtc tcagcctggt ggctatgacc gtcgcagcaa gtgttcaggc taaaactctg
    13921 gtttattgct cagaaggatc tccggaaggg tttaacccgc agctgtttac ctccggcacc
    13981 acctatgacg cctcttccgt cccgctttat aaccgcctgg ttgaatttaa aatcggcacc
    14041 accgaagtga tcccgggcct cgctgaaaag tgggaagtca gcgaagacgg taaaacctat
    14101 accttccatc tgcgtaaagg tgtgaagtgg cacgacaata aagaattcaa accgacgcgc
    14161 gaaatgaacg ccgatgacgt ggtgttctcg ttcgatcgtc agaaaaacgc gcaaaacccg
    14221 taccataaag tttctggcgg cagctacgaa tacttcgaag gtatgggctt gccggagctg
    14281 atcagcgaag tgaaaaaggt ggacgataac accgttcagt ttgtgctgac tcgaccggaa
    14341 gcgccgttcc tcgctgacct ggcaatggac ttcgcctcta ttctgtcaaa agaatatgct
    14401 gacgcgatga tgaaagccgg tacaccggaa aaactggacc tcaacccaat cggaaccggt
    14461 ccgttccagt tacagcagta ccaaaaagat tcccgtatcc gctacaaagc gtttgatggc
    14521 tactggggca ccaaaccgca gatcgatacg ctggtcttct ctattacccc tgatgcttcc
    14581 gtgcgttacg cgaaattgca gaagaatgaa tgccaggtga tgccgtaccc gaacccggca
    14641 gacatcgctc gcatgaagca ggataaatcc atcaacctga tggaaatgcc ggggctgaac
    14701 gtcggctacc tctcgtataa cgtgcagaaa aaaccgctgg atgacgtgaa agttcgccaa
    14761 gcactaacct acgcggtgaa caaagacgcg atcatcaaag cggtttatca gggcgcgggt
    14821 gtatcagcga aaaacctgat cccgccaacc atgtggggct ataacgacga cgttcaggat
    14881 tacacctacg atcctgaaaa agcgaaagcc ttgctgaaag aagcgggtct ggaaaaaggt
    14941 ttctccatcg acctgtgggc gatgccggta caacgtccgt ataacccgaa cgctcgccgc
    15001 atggcggaga tgattcaggc agactgggcg aaagtgggcg tgcaggccaa aatcgtcacc
    15061 tacgaatggg gtgagtacct caagcgtgcg aaagatggcg aacatcagac ggtaatgatg
    15121 ggctggactg gcgataacgg ggacccggat aacttcttcg ccaccctgtt cagctgcgcc
    15181 gcctctgaac aaggttctaa ctactcaaaa tggtgctaca aaccgtttga agatctgatt
    15241 caaccggcgc gtgctaccga cgaccataac aaacgcgttg aactgtacaa gcaagcgcag
    15301 gtggtgatgc acgatcaggc tccggcactg atcatcgctc actccaccgt gtttgaaccg
    15361 gtacgcaaag aagtcaaagg ctatgtggtt gatccattag gcaaacatca cttcgaaaac
    15421 gtctctatcg aataattaaa agccatacaa gactgatggc aaaggcaaaa ctgcctgatg
    15481 tgctccgctt atcaggccta cgaaaattct gcaatgtatt gaatttgcac gattttgtag
    15541 gccggataag gcgttaacgc cgcatccggc ataaacaaag cgcactttgt caacaatctg
    15601 tatacccggt ggcgctgtgc ctctgcgtgc agcgccatcc ggcagcaata cttcattctc
    15661 tgtccgacaa gggcgggaat tgatttgtga gcaatacaga cacgcagttc caggctgcga
    15721 gtcactatag agaatccggg ttatgttgca gtttattctc cgacgtttgg gactcgtcat
    15781 ccccacgttt atcggtatta cccttctcac atttgccttt gtccacatga tcccgggcga
    15841 tccggtgatg atcatggcag gcgaacgtgg gatctcccct gagcgtcacg tgcagctgct
    15901 ggctgaactc ggcttagata aaccgatgtg gcagcagtat ctccattaca tttggggcgt
    15961 aatgcacggc gatctaggca tttcaatgaa aagccgaatt ccagtatggg aagagttcgt
    16021 gccgcgcttt caggccacgc tggaacttgg cgtctgcgcg atgatttttg ctacggcagt
    16081 cggtattccg gttggtgtgc tggctgcggt taaacgcggt tccattttcg atcacactgc
    16141 agttggcctg gcgctgaccg gctactcgat gccgatcttc tggtggggca tgatgctgat
    16201 catgctggtt tcggtgcact ggaacctgac gcccgtctcc ggtcgcgtga gcgatatggt
    16261 gttcctcgat gactccaatc cgttaaccgg ttttatgcta atcgacaccg ccatctgggg
    16321 tgaagacggc aactttatcg atgccgtcgc ccatatgatc ttgcccgcca ttgtgctggg
    16381 tactattccg ctagcggtca ttgtgcgtat gacacgctcc tcgatgctgg aagtgctggg
    16441 cgaggattac atccgcaccg cgcgcgccaa agggctaacc cgcatgcggg tgattatcgt
    16501 ccatgcgctg cgtaacgcga tgctgccggt agtgaccgtt atcggcctcc aggtgggaac
    16561 attgctggcg ggggcgattc tgaccgaaac catcttctcg tggcccggtc tggggcgctg
    16621 gttgattgac gcactgcaac gccgcgacta tccggtagtg cagggcggcg tattgctggt
    16681 ggcgacgatg attatcctcg tcaacttgct ggtcgatctg ctgtacggcg tggtgaaccc
    16741 gcgtattcgt cataagaagt aaggggacat catgtcacag gttactgaaa ataaagtgat
    16801 tagcgcaccg gtgccgatga ccccgttaca ggagttctgg cactatttta aacgcaacaa
    16861 aggcgcggtc gtcgggctgg tttacgtcgc catcgtgctg ttcatcgcga tctttgccaa
    16921 ctggattgcg ccttataacc cggcggatca gttccgcgat gcgctgctcg ccccgccagc
    16981 ctggcaggaa ggcggcagta tggcacactt gctgggcacc gatgacgtcg gtcgtgatgt
    17041 gctgtcgcgg ctgatgtacg gcgcgcgttt atcgctgctg gttggctgtc tggtggtggt
    17101 gttgtcgctg attatgggcg ttattctcgg cctgatcgcc ggttacttcg gcggcctggt
    17161 tgataacatt atcatgcgcg tggtcgatat catgctggcg ctgccaagcc tgctgttggc
    17221 actggtgctg gtggcgattt tcggcccgtc gattggtaac gccgcgctgg cgctgacctt
    17281 cgttgccttg ccgcactatg tgcgcttaac ccgcgccgcc gtgctggtgg aggtgaatcg
    17341 cgattacgtc accgcctctc gcgtggcagg tgccggggcg atgcgccaga tgtttattaa
    17401 catcttcccg aactgccttg cgccgctgat tgttcaggcg tcgctcggtt tctctaacgc
    17461 cattctcgat atggctgctc ttggtttcct cggcatgggg gcacagccgc caacgcctga
    17521 gtggggcact atgctctccg acgtgttgca gttcgcgcaa agcgcctggt gggtcgtgac
    17581 cttccctggt ctggcgatcc tgttgacggt gctggcattt aacctgatgg gtgacggtct
    17641 gcgtgacgcg ctcgatccca aactaaagca gtaagaggtt cgagatggcg ttattaaatg
    17701 tagataaatt atcggtgcat ttcggcgacg aaagcgcacc gttccgcgcc gtagaccgca
    17761 tcagctacag cgtaaaacag ggcgaagtgg tcgggattgt gggtgagtcc ggctccggta
    17821 agtcggtcag ttcactggcg attatggggc tgattgatta tccgggccgc gtaatggcag
    17881 aaaaactgga gtttaacggc caggatttgc agcgtatctc agaaaaagag cgccgcaacc
    17941 tggtgggtgc cgaagtggcg atgatcttcc aggacccgat gaccagcctt aacccgtgct
    18001 acaccgtggg tttccagatt atggaagcga ttaaagtgca tcagggcggc aacaaaagta
    18061 cccgccgtca gcgggcgatt gacctgctga atcaggtcgg tattcccgat ccggcttcgc
    18121 gtctggatgt ttacccgcat cagctttccg gcggcatgag ccagcgcgtg atgatcgcca
    18181 tggcgattgc ctgtcggcca aaactgctga ttgccgatga accgaccacc gcgctggacg
    18241 tgaccattca ggcgcaaatc atcgaactac tgctggagct acagcagaaa gagaacatgg
    18301 cgctggtgtt aattacccat gacctggcgc tggtggcgga agctgcgcat aaaatcattg
    18361 tgatgtatgc aggtcaggta gtagaaacgg gcgatgcaca cgccatcttc catgcgccgc
    18421 gtcacccgta tactcaggca ttgctgcgtg cgctgccaga atttgctcag gacaaagaac
    18481 gtctggcgtc gttgccaggt gttgttcccg gcaagtatga ccgcccgaac ggctgcctgc
    18541 ttaacccgcg ctgcccctat gccactgaca gatgtcgcgc tgaagaaccg gcgctgaata
    18601 tgctcgctga tgggcgtcag tccaaatgcc attacccact tgatgatgcc gggaggccca
    18661 cactatgagt acgcaagagg ccacctcgca acaaccgctg ttgcaggcta tcgacctgaa
    18721 aaaacattat ccggtgaaga aaggcatgtt cgtgccggaa cgtctggtga aggcgctgga
    18781 cggcgtttcg tttaaccttg aacgtggcaa aacgctggca gtagtgggcg aatctggctg
    18841 cggtaaatcg accctcggtc ggttgctgac gatgatcgaa acgcccaccg gcggcgagct
    18901 gtattatcag gggcaggatc tgctcaagca cgatccgcag gcgcagaagc tgcgtcggca
    18961 gaaaatccag atcgtcttcc agaatcccta cggttcgctg aatccgcgta agaaagttgg
    19021 gcaaattctc gaagagccgc tgcttattaa caccagctta agcaaagaac agcgtcggga
    19081 aaaagccctg tcgatgatgg caaaagtcgg cctgaaaacc gagcactatg accgctatcc
    19141 gcacatgttt tccggcggac aacgtcagcg tatcgctatt gcccgtggtc tgatgctcga
    19201 cccggatgtg gtgattgccg atgagccggt ttctgcgctg gacgtctcag tgcgtgcgca
    19261 ggtgctgaat ctgatgatgg atttgcagca ggagttgggg ctttcttatg tctttatctc
    19321 ccacgacctg tcggttgtgg agcacattgc tgatgaagtg atggtgatgt acctaggccg
    19381 ctgcgtggag aaaggaacga aggaccaaat cttcaataac ccgcgccatc cgtacaccca
    19441 ggcgctgctc tccgcgacgc cacgcctgaa cccggacgat cgtcgtgaac gcatcaaact
    19501 caccggtgaa ctgccaagcc cgctgaatcc tccgccgggt tgcgccttca acgcccgctg
    19561 tcgtcggcgc ttcggcccct gcacccagtt gcagccgcag ctaaaagact acggaggtca
    19621 actggtagct tgttttgctg ttgatcagga tgaaaatccg cagcgttaac tcacaaaacc
    19681 ggggccatgc gctccggttt actcaatcag cttcaacagt ggcgacaagc atagcaaaat
    19741 gccgtaaagc agaatcagcc aggctttaaa tccgcgaagt ttttccagtt gtgcgacttt
    19801 atagatcagg aaaaacggaa tgaggcacga cacaatgcca tataacgggc ttcccaactg
    19861 aaagaacacc agcactgata cacgaaacga aacccaaatc gtcaacgtaa tgacgataaa
    19921 agcgcagatc gccagagtca gcatgcgtga gttaattttc ttggtatcaa taattcggct
    19981 taacagattg agaataatgc ctttaatagc ctcgtggaaa ccgagataaa tgccaaagaa
    20041 tgcggtcagt acggcaaaga tattcagcac cgtagaggtg atatgaatga tatgtccagg
    20101 gatcacctgc gcggccagtg ccagtgccga gatattttgt tcaaaggcag aaacggcttc
    20161 ttcgtggcta attgagaagg taaacgaaaa ggcaaaaaac aggatcaccg cgatgagcgt
    20221 gatataacta attcggtggg tacgcagcgc gagccgggtt gccagtactt tatctgcttc
    20281 ccgtttacga taggcaatat tcattgggtt aagtacctga ataaataccg cagaaaagaa
    20341 gcaaaatgga atggtaagca ggacatcgcg gaaaaagacg gaggcttgcg ggaaggcggt
    20401 tatattggcg aaattccagt gcgggatcat cgcaaaaccg aacacgacaa taatccctac
    20461 tttgaccacc accattggcc cagaaatctt aaacagtaat cgttcaccac cagacgcaat
    20521 cgctatcagt acggcgaaaa tagcgacttt ataaagtaga gattgtgaaa gatcggcatc
    20581 ggttaaaccg aaggttttca ggtacgaggc gctgtcgaaa accacggaga gagagtagat
    20641 aaaaatcccg tggataatca tcaaaaagta gataaccccg aggaaaattc cccagttctt
    20701 ccccaggtaa tgactgataa tatcggtgta gtcattacag gaatcgcttt cagaaagggt
    20761 ttttaaataa atgtcctgca ctacccaggt gacaggataa gcaatgatcg ctgcggtaat
    20821 aaatacccaa attcccttca agccaatttg tactggcatc agcacggttc cggcaccaat
    20881 cgccatgcct atgcataata aaacccagcc gaagtcgtag cgtgtaaacg gcaatttctg
    20941 attgtgtttc gatagtgtgt tgtgctgcat aaagtcctgt ccagaaagaa gtcggcctcg
    21001 caaaaatgaa taaacccagt ttctcttgcg caacaggtgg agaagtcgaa tacgcagtat
    21061 gtaaaaaatg aggcaacaga ttcactgggg aggtcgcgag gaggcgcaat tctacaaaat
    21121 tgcgcatttc actaccagtc tggagtcagc ccggatatga actgcaacta ttgcaaataa
    21181 agaaattaag ctggaaggca tcgcgcaatg aggaagattg ccgggtgctg acagtaaaaa
    21241 gca
//
LOCUS       DAJTQY010000065        20836 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.065, whole genome shotgun sequence.
ACCESSION   DAJTQY010000065 DAJTQY010000000
VERSION     DAJTQY010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20836
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.065"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(19..1017)
                     /gene="fbp"
                     /locus_tag="OO349_002572"
     CDS             complement(19..1017)
                     /gene="fbp"
                     /locus_tag="OO349_002572"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HCR5867598.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            1193..2566
                     /gene="mpl"
                     /locus_tag="OO349_002573"
     CDS             1193..2566
                     /gene="mpl"
                     /locus_tag="OO349_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HCR5867599.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(2649..3200)
                     /locus_tag="OO349_002574"
     CDS             complement(2649..3200)
                     /locus_tag="OO349_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HCR5867600.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRPAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            3294..4646
                     /gene="pmbA"
                     /locus_tag="OO349_002575"
     CDS             3294..4646
                     /gene="pmbA"
                     /locus_tag="OO349_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HCR5867601.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPNLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            4829..5215
                     /gene="cybC"
                     /locus_tag="OO349_002576"
     CDS             4829..5215
                     /gene="cybC"
                     /locus_tag="OO349_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HCR5867602.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVIEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(5260..5724)
                     /gene="nrdG"
                     /locus_tag="OO349_002577"
     CDS             complement(5260..5724)
                     /gene="nrdG"
                     /locus_tag="OO349_002577"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HCR5867603.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(5882..8020)
                     /gene="nrdD"
                     /locus_tag="OO349_002578"
     CDS             complement(5882..8020)
                     /gene="nrdD"
                     /locus_tag="OO349_002578"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HCR5867604.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVNEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVNVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFSGEHVY
                     DNEKLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(8414..10069)
                     /gene="treC"
                     /locus_tag="OO349_002579"
     CDS             complement(8414..10069)
                     /gene="treC"
                     /locus_tag="OO349_002579"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HCR5867605.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(10119..11540)
                     /gene="treB"
                     /locus_tag="OO349_002580"
     CDS             complement(10119..11540)
                     /gene="treB"
                     /locus_tag="OO349_002580"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HCR5867606.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(11659..12606)
                     /gene="treR"
                     /locus_tag="OO349_002581"
     CDS             complement(11659..12606)
                     /gene="treR"
                     /locus_tag="OO349_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HCR5867607.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMENQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            12791..12844
                     /gene="mgtL"
                     /locus_tag="OO349_002582"
     CDS             12791..12844
                     /gene="mgtL"
                     /locus_tag="OO349_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HCR5867608.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            12985..15681
                     /gene="mgtA"
                     /locus_tag="OO349_002583"
     CDS             12985..15681
                     /gene="mgtA"
                     /locus_tag="OO349_002583"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HCR5867609.1"
                     /translation="MFKEIFTRFIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGDNKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDTMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKLTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMVFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(15887..16273)
                     /gene="ridA"
                     /locus_tag="OO349_002584"
     CDS             complement(15887..16273)
                     /gene="ridA"
                     /locus_tag="OO349_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HCR5867610.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(16346..16807)
                     /gene="pyrI"
                     /locus_tag="OO349_002585"
     CDS             complement(16346..16807)
                     /gene="pyrI"
                     /locus_tag="OO349_002585"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HCR5867611.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEEQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(16820..17755)
                     /gene="pyrB"
                     /locus_tag="OO349_002586"
     CDS             complement(16820..17755)
                     /gene="pyrB"
                     /locus_tag="OO349_002586"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HCR5867612.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(17759..17893)
                     /gene="pyrL"
                     /locus_tag="OO349_002587"
     CDS             complement(17759..17893)
                     /gene="pyrL"
                     /locus_tag="OO349_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HCR5867613.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCLGVRR
                     "
     gene            complement(18174..18569)
                     /locus_tag="OO349_002588"
     CDS             complement(18174..18569)
                     /locus_tag="OO349_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCR5867614.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(18701..19414)
                     /gene="bdcA"
                     /locus_tag="OO349_002589"
     CDS             complement(18701..19414)
                     /gene="bdcA"
                     /locus_tag="OO349_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5867615.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDVNPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            19485..20078
                     /locus_tag="OO349_002590"
     CDS             19485..20078
                     /locus_tag="OO349_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR5867616.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVSEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAIQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTLLKE"
     gene            20223..20675
                     /gene="tabA"
                     /locus_tag="OO349_002591"
     CDS             20223..20675
                     /gene="tabA"
                     /locus_tag="OO349_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HCR5867617.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGAPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
BASE COUNT         4962 a         5226 c         5520 g         5128 t
ORIGIN      
        1 gtggagaggc gcaggagatt acgcgtccgg gaactcacgg ataaagcgtt cgacatcttc
       61 aaccatatgg tcgttgccga caaagaacga acggcgctgg tgcagagttt ccgggatgat
      121 atccagaata cgctctttgc catcgctcgc tttaccgccc gcttgttccg ccaggaatgc
      181 catcgggttg cactcataaa gcaaacgcag tttgccgtcc gggtggctgg cggtgcttgg
      241 gtagagataa ataccgcctt tcagcaggtt acggtggaaa tccgcaacca gtgaaccgat
      301 ataacgcgag gtatacgggc gattggtgga tttatcttct tcctggcaga atttaatgta
      361 cttcttcacc ccgttcggaa acttaatgta gtttccttcg ttgatggagt aggttttgcc
      421 tttctccggg aagcgcatcc gttcctggca caggcagaaa acgcccagcg aaggatcgta
      481 agtaaaggcg tgaacaccgc atccggtggt gtaaaccagc atggtagagg agccgtatac
      541 cacgtaacct gccgcaacct gtttgttacc aggctggagg aaatcttctt ccgttaccgg
      601 cgtgccaaca ggcgtaacgc ggcggtagat ggagaaaatg gtaccgacag agacgttaac
      661 atcgatgttg gacgagccat ccagggggtc catcagcacc acgtattttg cgtgttcaca
      721 gccttcaaag acgacaatct catcttcttc ttcagaggca atccccgcaa cgatatcgcg
      781 tgctttcagt gcggctttca gtttttcatt agcgaacaag tcgagttttt gctgaacctc
      841 gccctgcacg ttctcagcac cgctggcacc caggatatca accagtcctg ctttgttgat
      901 atcgcgatgg ataatcttgg cgcccagttt tattgccgac agcaaagcag tgagctcacc
      961 ggtagcatga gaaaactcgt gctgcttttc gacaataaat tcacctaacg ttttcataaa
     1021 actttccctg caatgtttat ggagtaaagc gaccgcaaca atcttaacaa ataatctcaa
     1081 tgttgcgctc aggtgaatcg cgccagcaaa ttgcggatta tcctgaaatg cgtttctcac
     1141 ttgcccgaca tatgcgtaaa atgagcggca gattaaaaaa ggatagtgac gtatgcgcat
     1201 tcatatttta ggaatttgtg gcacatttat gggcggtctg gcgatgctgg cgcgccagtt
     1261 aggccatgaa gtaacgggtt cggacgccaa tgtgtatccg ccgatgagca ccttacttga
     1321 gaagcaaggc attgagctga ttcagggtta cgatgccagc cagctcgagc cgcagccgga
     1381 tctggtgatt attggcaacg ccatgacccg tggaaatccg tgtgtggaag cggtactgga
     1441 aaaaaacatc ccttatatgt caggtccaca gtggctgcac gattttgtgc tgcgcgaccg
     1501 ctgggtgctg gccgttgccg gtacacacgg caaaaccacc accgcgggaa tggcgacctg
     1561 gattctggaa cagtgtggtt acaaaccggg atttgtgatc ggcggtgtgc cggggaactt
     1621 tgaggtttcg gcgcgtctgg gcgaaagcga cttctttgtt atcgaagcgg atgagtacga
     1681 ctgcgccttc ttcgacaaac gctctaaatt tgtccattac tgcccgcgta cgctgatcct
     1741 caacaacctt gagttcgatc acgccgatat ctttgacgac ctgaaagcga tccagaaaca
     1801 gttccaccat ctggtgcgta tcgttccggg gcagggccgt attatctggc cggaaaatga
     1861 catcaacctg aaacagacca tggcgatggg ctgctggagc gagcaggagc tggtgggtga
     1921 gcagggtcac tggcaggcga aaaagctgac caccgatgct tccgaatggg aagttttgct
     1981 ggatggcgaa aaagtgggcg aagtgaaatg gtcgctggta ggcgaacata atatgcacaa
     2041 tggcctgatg gcgattgcag cggctcgcca tgttggtgta gcgccggcag atgccgctaa
     2101 cgcgctgggt tcgtttatta atgctcgtcg ccgtctggag ttgcgtggtg aagcgaatgg
     2161 cgtcacggta tatgacgatt ttgcccatca cccgacggcg attctggcaa cgctggcggc
     2221 gctgcgtggc aaagttggtg gtacggcgcg cattattgct gtgctggaac cgcgctcgaa
     2281 taccatgaaa atggggatct gcaaagacga tctggcacct tcattaggtc gtgccgatga
     2341 agtcttcctg ctgcaaccgg cgcatattcc gtggcaggtg gcagaagtgg cagaagcctg
     2401 cgttcagcct gcacactgga gtggcgatgt ggatacgctg gcagatatgg tggtgaaaac
     2461 cgctcagcct ggcgaccata ttctggtgat gagcaacggc ggttttggtg ggatccatca
     2521 gaaactgctg gatggtctgg cgaagaaggc ggaagctgcg cagtaattcg gcttcagcct
     2581 gatatagcat tgccggataa ggcgtttacg ccgcatccgg caaaccatac tcacatcaac
     2641 aacgaaaatt acccttcatt ctccgccagt tcgcgcagat actggaaaat ctggcgtgcg
     2701 gatttcggcg gcttattccc ttctttctct ttcttcgcgt tgcggatcag tgtacgcagt
     2761 tgctgacgat cggcatcggg ccacagattc aatacttcag cgatggcgtc atcaccttga
     2821 tcgatcagac gatcgcgcag gttttccagt ttatggaaca gcaccacctg ctggttgtga
     2881 cggtttttca gcttatccag cgcctgacga ataggctcta cgtcgcgctg gcgcagcatc
     2941 ttaccgatga gctgcaactg gcggcggcga ccttccatct taatacgctg ggcgagctca
     3001 atagccgggc gcagatccgc atctaacggg atcttatcca gcgcgttttt ccccagatca
     3061 acaatttccg cgccaaggcg ttttagctcc tcggcatcac gtttaatttc acttttactg
     3121 acccagataa tttcatcgtc ttcgtcttcg atgtcatcac cgggaacgtc gtcgagccag
     3181 tcttcgggct gcttagtcat ctcaggctcc ttaaaaaaag aggctaatgt taccagttaa
     3241 gatgcgcact gaaaaacggt tctctgttag acttcagaga aactctctac attatggcac
     3301 ttgcaatgaa agtaatctct caagttgaag cgcagcgcaa gattctggaa gaagcagttt
     3361 ccactgcgct ggagttggcc tcaggcaaat cggacggtgc ggaagttgcc gtcagcaaga
     3421 ccaccggcat tagcgtaagc acgcgttatg gtgaagtgga gaatgttgaa ttcaatagtg
     3481 atggcgcgct ggggatcact gtttatcacc agaaccgtaa aggtagcgca tcgtctaccg
     3541 atttaagccc gcaggccatt gcccgcaccg tacaggcggc gctggatatt gcccgttata
     3601 cctcgccaga tccctgtgcc ggcgtggcag acaaagagct gctggccttt gacgcaccga
     3661 atctcgactt gttccaccct gcggaagttt ccccggatga agccattgaa ctggcggccc
     3721 gcgcagaaca ggcggcattg caggcggaca aacgcatcac caataccgaa ggtggcagct
     3781 ttaacagcca ctacggtgtc aaagtttttg gcaacagcca cggcatgttg cagggttact
     3841 gctcaacgcg tcattcgctc tccagctgtg taattgccga agaaaatggc gatatggagc
     3901 gtgattacgc ctacaccatt ggccgcgcga tgagcgatct gcaaacgcca gagtgggtcg
     3961 gggccgactg tgctcgccgt actttatcgc gcctgtcacc gcgtaaactc tccaccatga
     4021 aagcgccggt catttttgcc aatgaagtgg caaccgggct ttttggccat ctggtggggg
     4081 cgatagcggg tggctcggtt taccgtaaat ctacctttct gctggattcg ctgggtaaac
     4141 aaattctgcc ggactggctg accattgaag agcatccgca tctgctgaaa gggctggcgt
     4201 cgacgccatt cgacagcgaa ggtgtgcgca ccgagcgtcg cgatattatt aaagatggca
     4261 tcctgactca gtggctgctg accagctact cggcgcggaa actggggctg aaaagcaccg
     4321 gacatgcggg cggtattcac aactggcgga ttgccggaca agggctaagc ttcgaacaaa
     4381 tgctcaaaga gatgggcacc gggctggtgg tgacggagct gatgggccag ggcgtgagtg
     4441 ccattaccgg tgattattcc cgtggtgcag cgggcttctg ggtagagaat ggcgaaattc
     4501 agtatcccgt tagtgaaatc accatcgcag gtaatttaaa agatatgtgg cgcaatattg
     4561 tcaccgtcgg taacgatatt gaaacacgca gtaatataca gtgtggttct gtgctgttgc
     4621 cggagatgaa aatcgccggg cagtaaaaaa ctggcgcgac ctgccgcgct ataaactaaa
     4681 aaattacaca aaatcattcg cactgcatcg agacggaaag tgaatgaggg ccggggagcg
     4741 tactcgcagt acgtgacccg ggcgaatgag cgtagccgtc gatgaggcag tgtgaaggat
     4801 gaagtgtaaa taaaaaagga agtgagcaat gcgtaaaagc ctgttagcta ttcttgcagt
     4861 ctcctcgttg gtattcagtt ctgcgtcgtt tgccgctgat cttgaagaca atatggaaac
     4921 cctcaacgac aatttaaaag tgatcgaaaa agcggataac gcggcgcaag tcaaagacgc
     4981 gttaacgaag atgcgcgccg cagcgctgga tgcgcaaaaa gcaacgccgc cgaagctcga
     5041 agataaatca ccggacagcc cggaaatgaa agatttccgc cacggtttcg acattctggt
     5101 cggtcagatt gacgacgcgc tgaagctggc aaatgaaggt aaagtaaaag aagcgcaggc
     5161 tgctgcagag caactgaaaa cgacccgcaa cgcctatcac cagaagtatc gttaattcct
     5221 catttccctg ttgcctgcac tcaggtaaca gggaacttat catcgcaaat gatgcaccac
     5281 ctgattgctg ctgccgcgcc agatcaggga agggtctttt aaatcctgca caaatttgcc
     5341 gtcgaccagc acgttaatca aatcaacaac ctgcatttgc gcagcgttga gttcgtcgag
     5401 tttatagcct gtccacaccc agatgtcttt acccggacac tcggcgcgga tgcgttttac
     5461 cagtttcaga atatccggca cgttttgcgg atgcagcgga tcgccgccgg agagggaaat
     5521 cccctggcgt ttgatacgag tgtcattcag atctttaatg atcttgtctt ccattgcttt
     5581 ggtaaatggc tgaccagaat ttacccgcca tgtgcttttg ttatagcaac cggggcattc
     5641 atgaacacac ccggagacaa acagggtgca gcgagtgccg gggccgttga cgatgtcgac
     5701 aggatagtac tgatgataat tcattatttc gcctggctta tcagtccgcg tgtaggctgc
     5761 atccggtaat caatgcctga tgcgacgctg tcgcgtctta tcaggcctac agccgttgcc
     5821 aaatgtaggc cggataaggc gtttacgccg catccggcat ttgcttagcg cagagtgaag
     5881 attaacctat ctgcccattc cccaaatgtt taacgcggcg cttaacttct tcctgcttac
     5941 cagcgttaaa cggacgtgca tccgggctac ctaaatatcc gcacacgcgg cgagttaccg
     6001 acacacggga ggcgtcatgg ttaccacatt tcgggcaagt gaagcctttg ctggtgcact
     6061 cgaactcacc ggtaaagcca cactcgtagc actcatcaat cggtgtattg gtgccgtaat
     6121 acggtacatg ctgatagctg taatcccaga catcttccag cgctttcagg ttgtgctgaa
     6181 tgtttggata ctcgccgtag caaatgaaac caccgttcgc cagcggcggg taaggcgctt
     6241 caaagtcgat cttgtcgtac gggttcacct tcttctccac atcaaggtgg aaactgttgg
     6301 tgtagtaacc tttatcggtc acgcccggca ccacgccaaa ctcagcagta tcgagacggc
     6361 agaagcggtc gcacaggttt tcactcggcg tgctgtaaag actgaaacca taacccgttt
     6421 cttctttcca ctgatccact gcctgacgca gacgttcaac aatggcgata cctttcgcgc
     6481 gaagcttctc gttgtcgtaa acatgctcgc cgctgaacag cgcgttaatg gtttcgtgga
     6541 tgccgatgta acccagcgaa atagacgcac gaccgttttt gaagatttca gaaacatcat
     6601 cgtcagcatt cagacgtacg ccacaagcac cttccatata gaggatcggg gcgacgcgcg
     6661 ctttcacgcc ttcgagacga gcgatacggg tcatcagtgc cttacgtgcc agcaccagac
     6721 gttcatccag cagcttccag aaggtggctt catcgccttt tgcttccaga gcaatacgcg
     6781 gcaggttcag gctgatcacg ccgaggttgt tacgaccatc gtggatctgc tcgccgtttt
     6841 cattttccca cacgccgagg aagctgcggc agcccatcgg ggttttaaac gaaccggtga
     6901 cgttcactac ctgatcgtag ttcaggatat ccggatacat gcgcttgctt gcgcactcca
     6961 gcgccagctg tttgatgtcg tagttcggat cgcctttttt atggttcagg ccatcgcgaa
     7021 tcgcaaacac cagtttcggg aacaccgcag ttttacggtt tttaccgagg cctgcgatac
     7081 ggttacgcag gatcgattcc tgaatcaggc gcgattccca gctggtgccc agaccaaaac
     7141 caaaggttac aaacggcgtc tgaccgttgg cggtgtgcag ggtgtttact tcgtactcca
     7201 gcgactggaa ggcatcgtag cactctttga tggtacgaga gttagcgtag ccttcggcgt
     7261 ccgggatgtt ccactcttct gcggtcttgc gatgtttgtt gtagctggcg gtaacaaacg
     7321 gcgccagcac ttcatcgata cggttaatgg tggtgccgcc ataaatatgg ctggcgacct
     7381 gagcaataat ctgcgcagtt accgcggttg ccgtagagat cgacttcggc ggttcaatct
     7441 cggcgttccc cattttaaaa ccctgggtca gcatgccttt caggtcgatc aacatgcagt
     7501 tgaacatcgg gaagaacggc gagtaatcga gatcgtgata gtgaatatcg ccacgctcat
     7561 gtgcctgcac cacgtcacgc ggcagcaggt gctggcgtgc atagtgttta gccacgatcc
     7621 cggccagcag gtcgcgctgg gttggaatca ccttgctgtc tttgttggcg ttttcgttga
     7681 gtaacgaggc gttggtctgc tcgaccagac cacggatctc ctggttcagg cgaccgcgtt
     7741 tttcacgttc aatgtcgcga tcgtgacggt actcgatgta agcacgagcc agttgtttgt
     7801 atggacccga catcagctgg ttttcaactg cggtctggat ctcgttgata tccacctggt
     7861 tgcgcccctg catctgctcg ttgacaaccg cggcaacagt ggcgcaataa tcggcatcat
     7921 cgacttccgc tgctttagct gcacgcagaa tcgcttcttt gatgcgctct gatttaaacg
     7981 gcactttgca gccgtctcgt ttcatcacat gcggtgtcat gatcgctcca tatttttaag
     8041 aacaggttat ccacagaaat tgggaaaggc gttttccggt tttttcgttt cgttttccgg
     8101 tgctttccgc aatcgccatc cgcttatcca caacgccgca cctttttcgt ggggagctgt
     8161 agtagcaatt atagtcgatt aatacaacat attgggttgg gatgcatttt aaagtctata
     8221 tatagtgctt tgcatcaagg atgttttaac tttttttgat gtagctcaaa gtaaaaagca
     8281 gagcgtacgg atgacgggcg ctacagcgtt atgtaaattt tttaatgaat ttgctggttg
     8341 aaaaatcaac aaaaacaaca tgctgacaga caaaaccccg gaatgaccgg cattatcggg
     8401 gcttagggaa gatttacttc tgtaaccacc agacagcctc aaaaggccgt aaattcatgg
     8461 cacagggttg tggtgaggct tcttcgtaat tatgcatcac aagctgccag ttgccgcgca
     8521 tttgccctgg ctgccagggt tggatctcac ggctaaggtt ggcaatgacc agcaaggttt
     8581 gccccttcca ttcacggcga tagcaccaca atacagggct gtttggcagc agatcctggt
     8641 aattgcccca tgtcaggatg gcttcttgct tacgcagtgc gattaacttt tggtaggtgt
     8701 aaaacacaga ggagtcatcg gccagcgcgg cttctacgtt gatttgttga tagttatcgc
     8761 ctaggccaat ccacggttcg ccagccgtaa acccggcatt atcgccgttg ctccattgca
     8821 tgggcgtgcg gctgttgtca cgggatttac tggcgaggat tgccaataac tcgtcggcat
     8881 cacgaccatc gttgcgcagc tcggcaaaca tattgaggct ctccacgtcg cgatagtcag
     8941 taatgcgcgt gaaatgcggg ttggtcatgc caatctcttc gccctggtag atatacggcg
     9001 ttccctgcat gccatgcagc accatcgcca gcatttttgc cgcaggcacg cggtattcac
     9061 cttcatcacc aaagcgagaa acaatgcgcg gctgatcgtg gttacaccag aacaaggcat
     9121 tccatgccac gttgtgcatt ccttgttgcc agtggcggaa caatgttttc aacgccacaa
     9181 agtcaggttt agccagcgtc catttttcac caccgggata atcgaccttc aggtgatgaa
     9241 aattaaaggt catcgacaat tcactgcctg tcagtgccgc gtatcgctgg caatgctcaa
     9301 ggctggtgga ggacatttca cctacggtca ttaacccgcg tggcgtaaac acatcgcggt
     9361 tcatctcgtg caaaaactcg tgtgctcgtg gcccgtcggt gtagaagcga cgcccgtcgc
     9421 catccaggtc ttcagggaaa cgcgggtctt tggagatcag attcaccaca tccaggcgca
     9481 acccgtcgac cccacgatcg gcccagaatt cacagacttt tttcagctct gcgcgtaccg
     9541 ctggattctc ccagttgaga tccgcctgtt ctggtgcaaa gagatgcaaa tagtactgtt
     9601 cgctttccgc atgccagcgc cacgcactac cgccaaattt tgaacgccag ttgttcggtg
     9661 gcgtttctgg ttcgccatcg cgccagatat aaaactggcg gtaagggctt tctttgttca
     9721 gcgcctcgcg aaaccaggca tgttgggtag aggtatggtt aaacaccata tcgagaatga
     9781 tacgaatccc gcgcgatttt gcctgcgtca ccagttcgtc aaaatcgtcc agcgtgccgt
     9841 aggtgggatc aatcgccgta tagttcgcta catcgtaacc gttatcgacc tggggagaga
     9901 cataaaaggg ggttagccag atggcatcaa cgcccagttt atgcagatag tccaggcgtt
     9961 ggataacgcc acgtaaatcg ccggtgccgc tacccgtggt gtcctgaaaa ctctttggat
    10021 aaatctggta gataacgccg ttttgccacc agtggggaag attagtcatt acgttattcc
    10081 tgcaaatgcg agggggcgca attgcgcccc gaagaaaatt aaacaatgtc cagcgtgccc
    10141 aggcggtatt tccgctgata gataaacgag gtgagtacaa tcgggatgat gatggcgata
    10201 gccattgcca gcgcaaatac ctgccagtag ctcggttgaa tagagagaat tcccggcagg
    10261 ccccctacgc cgataccatt cgccataacg ccgttcaggc cgcatagcaa tcctgccaga
    10321 ccagaaccaa tcatcgcgca cagcatcggg aagcgatatt tcaggttgat gccgtacatt
    10381 gcaggctcag tgaccccaag ccaggcggag atagcggcag gcacggagat ctcgcgttca
    10441 ttgtgcttgc ggctggaaat gataatgcct atcacggcgg agccctgagc gatattcgac
    10501 agcgcaatca gcggccacac tggcgtgcca cccatgcttt gaatcatctg caaatcaata
    10561 gcaagggtgg tctggtgtac gccggtgatc accagcggcg cgtacaggaa gccaaacaat
    10621 gccgcaccaa tcggagcaaa gctgccggtc atcaggtgac gtaccgcaaa ggcaacgcca
    10681 tcgccaatca tgcgaccaaa cggaccaatc agcgcatggg cgaggaacac cgcgaggatc
    10741 agcgaacaga cgggcaccac caccagatag aggtaatccg gtacgatgcg tttaaggcga
    10801 gtttcaataa cgcccagcgc cagcccggct aacagtgccg ggatcacctg cgcctgatag
    10861 cccactttgg cgatgctgaa catgccaaag tcccacactt ccggcagctg ctgcccgagc
    10921 agataagcgt tcatcagctg gggggaaacc agtgtcacgc caagcacgat accaaggatc
    10981 ggcgtgccgc ccattttttt caccgctgac cagcaaatcc cgaccggcag gtagaagaaa
    11041 atcgcttcac cgatcaacca cagaaaatcg tagatcgttt gcagggaagg gtacatttgc
    11101 gccagcgtct gaccgttgct catgggcaaa tcgccgatca cattgcgaaa accgaggatc
    11161 aaaccgccgc taatcaacgc gggcagcaac gggaagaaga tctccgcgaa atgagagatc
    11221 aactgctcat gccatttcat attttgccgg gcggcttttt ttacctgctc tttatcaacc
    11281 tgcgcctgtc cggttgacgc tattagtgct tgatagtaat cacccacgtt ggtgccaatc
    11341 accacctgaa attgcccggc attggtgaaa cagcctttca ccatggggag ttgctcaatt
    11401 tctttcggtc tggcattggc cggttggttg aggacaaagc gcaggcgagt aatacagtgg
    11461 ctcaccgtcg caatattgcc gcgcccgccg accagttcaa tcaaccgatc gatatccgtt
    11521 tggtttattt tgctcatcat aaagccccat ggcagatgac atttttggtt ggctgcagaa
    11581 tatattgcgc ggaaaaattt aaaaacggga acgttcccga aacgcagcga agatcacaat
    11641 ttatcgttca ggaaacgatc aggacagggt ggcggggatg atgatttgtt gcggttcgct
    11701 gcgcccggtt acctgtgcga tcaattggca ggccgcctgg cgtccagctt cggcgtagcc
    11761 aggatctacg gtgacgatct ccggatgaag gaatttcatt aacggcgtat tacctacgct
    11821 cgccagttgc aaggtgtcga tgcgttgctc ttgcaggtat ttacttgcgc caagagccag
    11881 cgtgtcggtt gcgcacagta acgcggtagt ttcaggcgta atcacttttg ctacgttttc
    11941 atagccttgc ttcatagcaa gccctggcag agcggcaacg ggatgcagtt tatgcgcttt
    12001 gcagaacgcc aggtaggctt cgtgacgtcg cttcccggtc gtcacgtcac tatgcggcac
    12061 gccgagataa ctgatattgc gatgcccctg gtcatagagc cgttgcatca gaatcttgat
    12121 tgccccttcg tcgtcataac agaccgaagc aaagcctttt gcgtcacgcg ccagcagaac
    12181 cagcgatgac tgccagtggg ctaacatttc ttcggttatg ccggtaaaac cgaacagcac
    12241 tacgccgtcg atattacgcc gtttcagcac ccccaaatgt tcggcaacta attgtgggga
    12301 aaactgattt tccatcatga ttggatcgta accttgttca tagaacgctg gcagcatggt
    12361 ttgaacggcg agattttctg acaacgaatc cagacgggta acaatgatgg cgaccacttt
    12421 atcgctttgc ccacgcatag cgcgcgcaga gcgggaaggg gaaaatccat gctgattcat
    12481 cactgcttca acacgctcgc gcgtgcgctg actcacgccg ctttcgttat tcagcacccg
    12541 ggaaaccgta gatttcccca cgccgcttaa gcgcgcgatg tctttgatgg tcagccgatt
    12601 ttgcatcctg ttgtcccata acgtgttgtt taattatttg agcctaactt tacccgtgca
    12661 ttcagcaatg ggtaaagtct ggtttatcgt tggtttagtt gccagcaggt attatatcgc
    12721 cataaatgct acgaatatta ttggattctc cttattattt gcggcgcttt tttcacttac
    12781 cggaggttat atggaacctg atcccacgcc tctccctcga cggagattaa aacttttccg
    12841 gtaagcccgt cttttcacgg cgttaccgga tgcgtaaggc cgtgacgttt taatgtccct
    12901 gctcagcttt attaccttca ggtaaggctt cgccacgcct gaagacattt ctgtactgtt
    12961 tcagacagtg cggagggact ccttatgttt aaagaaattt ttacccggtt cattcgccat
    13021 ttaccttccc gtctggttca tcgcgatcca ttgcctggcg cgcagcagac agtgaatacg
    13081 gcggtcccgc cgtccttaag tgcgcattgc ctgaaaatgg cggtgatgcc cgaagaagaa
    13141 ttgtggaaaa cgttcgacac ccatccggaa ggattaaatc aggcggaagt ggaatctgcc
    13201 cgcgaacaac atggtgataa taaattaccc gcccaacaac cgtcgccgtg gtgggtacat
    13261 ttatgggtct gctatcgcaa cccctttaat attttgctca ccattcttgg tgctatttct
    13321 tacgccacgg aagatttatt tgccgcaggt gttatcgcgc taatggtcgc tatttctacg
    13381 ttgctgaact ttattcagga agctcgttcc accaaagcgg cagatgccct gaaagcgatg
    13441 gtcagtaata ccgcgacggt actgcgagta attaacgaca aaggcgaaaa tggctggctg
    13501 gagatcccga tagaccagct ggtgcccggc gatattataa aactggcggc gggggatatg
    13561 atcccggcag atttacgtat cttgcaggcg cgggatctgt tcgtcgctca ggcgtcgtta
    13621 accggtgagt ctctgcccgt ggaaaaagcc gctactactc gccagccgga gcacagcaat
    13681 ccgctggagt gcgacaccct gtgttttatg ggcaccaccg tggtgagcgg cacggcacaa
    13741 gcaatggtga ttgctacagg agccaacacc tggtttggtc aactggcggg gcgtgttagt
    13801 gagcaggaaa gcgagccgaa tgcctttcag caagggatca gccgcgttag tatgctgctg
    13861 attcgcttta tgctggtgat ggctccggtg gtgttgctta tcaacggtta caccaaaggc
    13921 gactggtggg aagcggcact gtttgcgctt tcggtcgcgg taggcttaac gccggaaatg
    13981 ttgccgatga ttgttacctc gacgctggcg cgcggcgcag taaagctgtc gaaacagaaa
    14041 gtgatcgtca aacatctgga tgccattcag aactttggcg caatggatat tctgtgcact
    14101 gataaaaccg gcaccctgac gcaggacaaa attgtgctgg agaatcatac cgatatctcc
    14161 ggtaaaacca gcgaacgcgt gctgcatagc gcgtggttga acagtcatta ccagaccgga
    14221 cttaaaaatc tgcttgatac cgcggtgctc gaaggtacgg atgaagagtc agcgcgttcg
    14281 ctggccagcc gttggcaaaa aattgatgag attccgtttg atttcgagcg tcgtcggatg
    14341 tcggtggtag tggcagaaaa taccgaacac catcagctgg tttgcaaagg tgcattgcag
    14401 gaaatcctca atgtgtgttc gcaggtgcgt cacaatggcg agattgtacc gctcgatgac
    14461 accatgctgc gtaagattaa gcgggttact gatacgctga atcgtcaggg gctgcgcgtg
    14521 gttgcggtgg cgacgaaata cctgccagcg cgtgaaggag attaccagcg ggcggatgaa
    14581 tccgacctga tcctcgaagg atatattgct ttccttgatc cgccaaaaga gacaaccgct
    14641 ccggcactga aggcattaaa agcgagtgga attaccgtaa aaatccttac tggcgacagt
    14701 gagttagttg cagcgaaagt gtgccatgaa gtggggctgg atgcgggaga ggtggtcatt
    14761 ggtagtgata ttgaaacgct atctgacgat gaactggcaa atctcgcaca gcgcaccacg
    14821 ctgtttgccc gcctgacgcc gatgcataaa gaacgcattg tgactttact gaagcgcgaa
    14881 gggcatgtgg ttggctttat gggcgatggt attaatgatg cgcccgcctt acgcgctgcg
    14941 gatatcggca tttctgtgga cggcgcggta gatattgccc gtgaagcggc tgatatcatc
    15001 ctgctggaaa aaagcctgat ggtgctggaa gagggggtta ttgagggacg tcgcactttc
    15061 gccaacatgc tgaaatacat caaattgacg gcgagttcta acttcggtaa tgtgttcagc
    15121 gtgctggtgg cgagtgcttt cttgcccttc ctgccgatgt tgccgttaca cttgcttatt
    15181 cagaacctgc tgtacgatgt gtcacaggtg gcgatcccgt ttgataacgt cgacgacgag
    15241 caaattcaaa agccgcagcg ttggaatccg gcggatctgg ggcgctttat ggtcttcttc
    15301 ggaccgatca gctcgatttt cgatattctg acgttttgct tgatgtggtg ggtattccat
    15361 gccaacacgc cggaaacgca aacgctgttc cagtcgggct ggtttgtggt gggattactg
    15421 tcgcaaacgc tgattgtgca tatgatccgt acccgccgtg tgccgtttat tcagagctgt
    15481 gcatcgtggc cgttaatgat catgaccgtg atcgtgatga ttgtcgggat cgcattgccg
    15541 ttttcaccgc tggccagtta tctgcagctg caggcgctgc cgttaagcta tttcccgtgg
    15601 ctggttgcga ttctggcagg gtatatgacg ttaacccagt tggtgaaagg gttctatagc
    15661 cgtcgttacg gctggcagta aagaataaac tgggcatggt ggcccggatt cagattgcag
    15721 acaaagtgag ctttgttcat gccggatgcg gcgtaaacgc ccaatccggc ctacaaattc
    15781 aataaattgc aggaatcacg taggcctgat aagcgtagcg catcgggcga ttttgctttt
    15841 gtcatcagac ttaatccggg catgatagcc cggatttcca tcaagattag cgacgaacag
    15901 cgattgcttc gatctcaatc ttcacgtctt tcggcagacg ggcaacttca acgcaagaac
    15961 gtgccgggaa agtggcgttg tgttcggtga agaaggcttc gtaagtggcg tttacggttg
    16021 cgaagtcgtt cagatctttc acgaacacag tggttttaac gatgtcgccc actttcaggc
    16081 cagcggcttc gacgattgct tttacgttat ccagcgactg acgtgcctgt gcagcgacgt
    16141 ctgccggtac ttcaccagtt ttcggattta ccgggatctg accggaggtg atgatcatat
    16201 tgcccagatc aacgccctgt acgtaaggac cgatagctgc cggtgcattt tccgtcgcga
    16261 tagttttgct catgatttct cctttattac agcggtaaaa agtctggctc attataggga
    16321 gccagacttt tattaccaac cgcaattaat tggccagcac cacattatgg gaaaactctt
    16381 tttcacagta tttgcattta agcgcgatgt cattggcgcg ttttcgcacg gcaaagctgg
    16441 atgaaaccgg ttcggcatgg ctgatacagt tgctgttcgg gcagaccagc acattgtcga
    16501 tgcgctctgg cagacttggg cgcgatttac ccaccacttc atagttgtcg atacggttaa
    16561 ccgtggcttg cggtgcatac aatgccagtt ggtctacttg ctcttcactc aaaaaggtat
    16621 tttcgatttt gatcagatct ttgcggccca tctcgccaga aggcaaattc agaccaatgg
    16681 tgatgcgctg atctgtttcg gtcaacttga acagactcaa cagcttaaaa ccgatctggg
    16741 cggggatatg gtcaattacc gtgccgcgtt taatagcttc aacctgcaat ttattatcgt
    16801 gtgtcatctc tatttcccct tacagtacca gatcgcgatt cagaaccagt gccagtaacg
    16861 cctggcgagc gaaaatcccg ttgcctgctt gctggaagta ccaggcgtga ggcgttttat
    16921 ccacatccgt cgcaatctca tcaacacgcg gcagcggatg cagcactttc atattggctt
    16981 tggcgttgtg gagatcgctg gcgcgaagaa caaactgcgc tttcacgttg gcgtactcgg
    17041 acgggtccag acgctctttt tgcacgcggg tcatgtacag gatgtctact tccgccatca
    17101 cttcttcaat agagctgtgc agactccagg cgatcccttt ttcatcgagc atatccagaa
    17161 tgtattgcgg cattgccagc gcatccggcg cgataaagta aaaacggttg ccgtcgaact
    17221 tcgctagcgc ctgggtcagg gagtgaacgg tgcggccata tttcaggtca ccaaccattg
    17281 cgacgtggag attgtccaga cgcccctggg tttcctgaat ggtgaataag tccagcaagg
    17341 tttgcgtcgg atgttggttg gagccatcac cggcattcag taccggtaca ttgccagaaa
    17401 attcggtggc taggcgcgcc gcaccttcct gcggatgacg catcactatc gcatcgacgt
    17461 aagtgctgat aaccgaaatg gtatcggcca gcgtttcgcc ttttttaccc agtgatgtat
    17521 tggcgctgtc ggagaagccc accacgctgg cacccaggcg gtgcatggaa gtttcgaaag
    17581 agaggcgggt acgggtagag gcttcgaaga aacagctggc aatgactttg tgcttcaaca
    17641 gctctggttg cgggtttgct ttcagtttcg ccgctgtcgc cagcaccaga ttaaggtcat
    17701 cgcgactaag gtcgtttatg gaaatgatat gtttctgata tagcggatta gccatctttt
    17761 atctcctgac acctaggcaa aaaaaagccc ctcgattgag gggctgggaa tgggtgatca
    17821 acgggaagaa aaacggcagg ccagcgtctt ttttcagacg cggtaagaca aaatgtcgaa
    17881 cacactgaac catacatcct cccggcaaat tgtccggcat tatactcata gccagaagcg
    17941 gtgcaagcat ttgatgcaat attttgtcag cgcaaacggt ttatttgaat taaaagtcaa
    18001 aatatatgca tttttatttt ctatgattct ggaggggaag tgtcgttcgg tatcaataat
    18061 tgcagacatt tcctgctttt ttctttttca ttttcacgca atcagaaatg cattttattc
    18121 cattcatccg acttataggg cggagggtga aaacgaacgg ctaacactat tgcttatagc
    18181 tcagggatac gcgctatcac tttaatttca aaatcaaagc ctgccagcca ggtaacaccc
    18241 accgccgtcc agtttggata aggtggggcg ctaaatattt catttttcac cgtcatgatg
    18301 tcttcaaatt gtttttccgg atcggtatga aagctcgtaa catcaatgat atcgtcaaaa
    18361 gtgcatcccg cagctgccag ggtcgcatgc aaattatcaa aggccagtct gacttgttgc
    18421 tgaaaatcgg gttctggtgt tccgtcctct cgacttccta cttgcccgga aacaaacagc
    18481 aaatcgccgg aacgaatagc cgcagaataa cgatgctcag catacagtga atgtcggcca
    18541 gcagggaaaa cagcggttct ttctaccatt tggttatcct caagatttac gacatgaaca
    18601 gaagatttat atttatcggg agccgctttt agggggacga cgttagtaaa caaaacccag
    18661 acatcatgga taatggctgg gcttaattga gcgtagtcgg ttatgcgcca aacgcgccat
    18721 caatggtatg catcgcgccg gtaacaaaac tggcttctgg ccctgctaac catgcgacca
    18781 taccagcgac ctcttccggt tgcccatgtc ttttgatggc cataaaacta tgcaacatat
    18841 cgcgcattgg cccgttggcg ggattaacgt cggtatcaat tggtcctggc tggacgacgt
    18901 tgatggtgat ccctcgcggt ccaaaatcac gggccagccc gcgcgccatg ccttgcaggg
    18961 cagatttact ggcggcataa gcagccatgc ctgcaacagg catacgatcg ccattcacgg
    19021 agccgatgat taagatgcgc ccgccttcgg gcatctgccg ggcggcttca acagaggcat
    19081 gataaggagc atgaatattg attttgaaaa ggcgatcaat atcgtcggca tttaattcca
    19141 gggcatcgcc aaagacgcca atacctgcat ttaccaccag gatatccaat gcgccgctct
    19201 tacgaacgac atcaataaca gcgtctctgt cagcactatc tgtgaatact gctgtcgctc
    19261 cagtctcttg tgccagacgt tcagcggcat ctttcgaccc cgcataggtg aatcgtacat
    19321 tggccccatc ggtgacgaaa cgacgtacga tagcggcacc gattccacga ctgccaccga
    19381 ggatgagaac tgtcttaccg gtaaaagcgc ccataaggac tccttgattt attatgtaac
    19441 atgcattaca aaactgtttt aactttctgt caacaggttt tgtaatggtc actaaaaaac
    19501 aatctcgcgt tccaggtcgt cccagacgtt tcgctcctga gcaggcagtc tctgcggcaa
    19561 aagtgctttt tcaccaaaaa ggtttcgatg cagtaagtgt ctctgaagtt actgattatc
    19621 ttggtattaa ccccccgagt ctctacgcgg cttttggcag taaggctggg ttatttagcc
    19681 gtgtactcaa tgaatatgtc ggtacggaag ctattccgct tgccgatatt cttcgtgatg
    19741 atcgtccggt aggcgagtgc ctggttgagg tattaaaaga agcggcgcgc agatatagcc
    19801 aaaacggcgg ctgcgctggc tgtatggttc ttgaaggtat tcatagtcat gatccacaag
    19861 cgcgtgatat tgccattcaa tattatcacg ccgcagaaac gaccatttat gactatatcg
    19921 ccaggcggca tccacaaagc gcacaatgtg tgactgattt tatgagtacc gtgatgtcag
    19981 ggctttctgc gaaggcacgg gaggggcact ccatcgaaca actctgtgca acagctgccc
    20041 tggcagggga agcgataaaa actcttctca aggagtgatg ctggccttga tccgaaaggc
    20101 gggaacgcgc ctgccgataa gttgtgataa gacaataatt cacgcgttaa ggctggcgga
    20161 attgatcatc ttttcgtata acgatagaaa tgaaacatta ttttaattaa ggagtggaaa
    20221 agatgatcat cggaaatatt cataatcttc agccgtggct accccaggag ttacgccagg
    20281 cgattgagca tatcaaagca cacgttacgg cagaaacgcc aaagggcaag catgatatcg
    20341 aaggcaatcg tctgttttat cttatctcgg aagatatgac cgagccgtac gaagctcgcc
    20401 gtgcggagta ccatgcccgc tatcttgaca ttcagattgt gttaaaaggt caggaaggca
    20461 tgaccttcag cacgcaacct gcaggcgcgc cggataccga ctggttagct gataaagaca
    20521 tcgcattttt gccggaaggc gttgatgaga aaacagttat cctaaatgaa ggtgattttg
    20581 ttgtgtttta tccgggggaa gtgcataaac cgctgtgcgc agtgggcgcg ccagcccagg
    20641 ttcgcaaagc agtagtgaag atgctgatgg cgtgatgact tttcgccgta aataactcca
    20701 ggtttacggc gagtttgtga aaagagcgtt ttttgatatt tttttgtgag taaaatttgt
    20761 aatgcttaga cgttcttatt aactcaagga gttcgtcatg ggcaaaatat cagatttgaa
    20821 ttattctcaa cacatt
//
LOCUS       DAJTQY010000066        20810 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.066, whole genome shotgun sequence.
ACCESSION   DAJTQY010000066 DAJTQY010000000
VERSION     DAJTQY010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20810
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.066"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            80..907
                     /gene="mepH"
                     /locus_tag="OO349_002592"
     CDS             80..907
                     /gene="mepH"
                     /locus_tag="OO349_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HCR5867618.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSMTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPHTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAGPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            1035..1616
                     /gene="sodB"
                     /locus_tag="OO349_002593"
     CDS             1035..1616
                     /gene="sodB"
                     /locus_tag="OO349_002593"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HCR5867619.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(1762..2931)
                     /locus_tag="OO349_002594"
     CDS             complement(1762..2931)
                     /locus_tag="OO349_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5867620.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGTGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMHVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVTGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(3097..3186)
                     /gene="ynhF"
                     /locus_tag="OO349_002595"
     CDS             complement(3097..3186)
                     /gene="ynhF"
                     /locus_tag="OO349_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HCR5867621.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            3485..4510
                     /gene="purR"
                     /locus_tag="OO349_002596"
     CDS             3485..4510
                     /gene="purR"
                     /locus_tag="OO349_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HCR5867622.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(4507..5439)
                     /locus_tag="OO349_002597"
     CDS             complement(4507..5439)
                     /locus_tag="OO349_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5867623.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            5552..6763
                     /locus_tag="OO349_002598"
     CDS             5552..6763
                     /locus_tag="OO349_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HCR5867624.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVDCPNH
                     GNAEVAHSESH"
     gene            7054..8202
                     /gene="cfa"
                     /locus_tag="OO349_002599"
     CDS             7054..8202
                     /gene="cfa"
                     /locus_tag="OO349_002599"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HCR5867625.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCKGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(8242..8883)
                     /gene="ribE"
                     /locus_tag="OO349_002600"
     CDS             complement(8242..8883)
                     /gene="ribE"
                     /locus_tag="OO349_002600"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HCR5867626.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            9098..10471
                     /gene="mdtK"
                     /locus_tag="OO349_002601"
     CDS             9098..10471
                     /gene="mdtK"
                     /locus_tag="OO349_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HCR5867627.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRYMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPEPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            complement(10512..11768)
                     /locus_tag="OO349_002602"
     CDS             complement(10512..11768)
                     /locus_tag="OO349_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HCR5867628.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVLGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPSGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            12076..12152
                     /locus_tag="OO349_002603"
     tRNA            12076..12152
                     /locus_tag="OO349_002603"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12110..12112,aa:Val,seq:gac)
     gene            12157..12233
                     /locus_tag="OO349_002604"
     tRNA            12157..12233
                     /locus_tag="OO349_002604"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12191..12193,aa:Val,seq:gac)
     gene            12341..12646
                     /locus_tag="OO349_002605"
     CDS             12341..12646
                     /locus_tag="OO349_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HCR5867629.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            12772..14376
                     /locus_tag="OO349_002606"
     CDS             12772..14376
                     /locus_tag="OO349_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HCR5867630.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFHRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EEGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYEMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(14388..15200)
                     /locus_tag="OO349_002607"
     CDS             complement(14388..15200)
                     /locus_tag="OO349_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5867631.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIRHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGLGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(15204..15989)
                     /locus_tag="OO349_002608"
     CDS             complement(15204..15989)
                     /locus_tag="OO349_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HCR5867632.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFAMVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGDNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(15986..16654)
                     /locus_tag="OO349_002609"
     CDS             complement(15986..16654)
                     /locus_tag="OO349_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HCR5867633.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQDNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(16718..17356)
                     /locus_tag="OO349_002610"
     CDS             complement(16718..17356)
                     /locus_tag="OO349_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HCR5867634.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARTVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(17369..19470)
                     /locus_tag="OO349_002611"
                     /pseudo
     CDS             complement(17369..19470)
                     /locus_tag="OO349_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
     gene            complement(19491..20117)
                     /locus_tag="OO349_002612"
     CDS             complement(19491..20117)
                     /locus_tag="OO349_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HCR5867635.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            complement(20573..20782)
                     /gene="fumD"
                     /locus_tag="OO349_002613"
     CDS             complement(20573..20782)
                     /gene="fumD"
                     /locus_tag="OO349_002613"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HCR5867636.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
BASE COUNT         5220 a         5399 c         4838 g         5353 t
ORIGIN      
        1 ttcttctatg cattagaatc atcaagtttt gtaaatcaga cgcaggcatg atagacctgc
       61 cttaacagag ggacgctcag tggcgcggat aaaccgtatt tcgatcacgc tctgtgcttt
      121 actttttacc accctgcctt taacgcctat ggcccatgct tcaaagcaag ccagggagag
      181 ttctgctacc actcatatca ccaaaaaagc agataaaaag aaaagcacgg caaccaccaa
      241 aaagacccag aaagccagcc agaaaacagc gaaaaaagcc gccagtaaaa gtatgaccaa
      301 aagcaaaacc gcttcttctg ttaaaaaatc ttccattacc gcttctaaaa acgccaaaac
      361 tcgcagcaaa cacgccgtca ataaaacggc ctcagccagc tttaccgaaa agtgtaccaa
      421 acgtaagggc tataaatcgc attgtgtgaa agtcaaaaat gccgcgtcag gaacccttgc
      481 cgacgcgcac aaagcgaagg tgcaaaaagc cacgaaagtg gcaatgaata aactgatgca
      541 gcaaattggt aagccatatc gttggggcgg cagctcaccg cataccggtt ttgattgcag
      601 cggcctggtt tattacgctt ataaagattt ggtgaaaatt cgtattccgc gtacggcgaa
      661 tgaaatgtat cacctgcgtg atgcaggacc tatcgaacgt agtgagctga aaaacggcga
      721 cctggtcttt ttccgtactc agggacgcgg tacagccgat catgtcggcg tgtatgtcgg
      781 caacggcaaa tttattcagt caccgcgcac aggtcaggaa attcaaatca cttctctcag
      841 tgaagactac tggcagcgcc actatgttgg cgctcgtcgg gtaatgaccc caaaaacact
      901 tcgctaaaac tttaccctgt tgttacggca acagggtaag ttcatctttt gtctcacctt
      961 ttaatttgct accctatcca tacgcacaat aaggctattg tacgtatgca aattaataat
     1021 aaaggagagt agcaatgtca ttcgaattac ctgcactacc atatgctaaa gatgctctgg
     1081 caccgcacat ttctgcggaa actatcgagt atcactacgg caagcatcat cagacttatg
     1141 tcactaacct gaacaacctg attaaaggta ccgcgtttga aggtaaatca ctggaagaga
     1201 ttattcgcag ctctgaaggt ggcgtattca acaacgcagc tcaggtctgg aaccatactt
     1261 tctactggaa ctgcctggca ccgaacgccg gtggcgaacc gactggaaaa gtcgctgaag
     1321 ctatcgccgc atcttttggc agctttgccg atttcaaagc gcagtttact gatgcagcga
     1381 tcaaaaactt tggttctggc tggacctggc tggtgaaaaa cagcgatggc aaactggcta
     1441 tcgtttcaac ctctaacgcg ggtactccgc tgaccaccga tgcgactccg ctgctgaccg
     1501 ttgatgtctg ggaacacgct tattacatcg actatcgcaa tgcacgtcct ggctatctgg
     1561 agcacttctg ggcgctggtg aactgggaat tcgtagcgaa aaatctcgct gcataataac
     1621 tgatggcaaa tgcagcattg cctgatgcgc ttcgcttacc atgccaacgc ggatgaggcg
     1681 ttaacagcgc attcggcaag ataaatcgca cgttgtcagc aactgtaacg cagaaggtta
     1741 tccttctgcg tttttgttta attagctgtt agcaacgcaa actgtttcag gttgttttct
     1801 ggctgacata aacaccagca ataatgccag tcccgcgaca atcgcccccg tcaccggcac
     1861 aaagctgtat cccagcccag cggaaattac cgcaccacca gcagctgctc ccagcgcatt
     1921 tccaagatta aaggcaccaa tattgactga tgaagacaga cctggcgctt cactggcgac
     1981 atgcatcacg cgcatctgta acggaggtac aaccgcaaag gttgctgcgc cccacaccac
     2041 catgctaata gctgcgccga actcattgcg ggccaggaac gggattgcca gcataatcac
     2101 catcagcagc aacaaaaagc ctttcaacgt gccgttaact gaacgatctg ccagtttgcc
     2161 gccgagatag ttaccgatag agaatccgac accaatcagc accagcattg ccgtgacgaa
     2221 caccggtgtt gcgtgggtaa tactttgcag taccggagag atataggtgt agagggtaaa
     2281 cattgcacca gctcccagta ccgtcgtcag caatgcagac agcacctgcg gacgcattaa
     2341 taccgccagc tcttttttca cttcaggtcg tgcccctgta ccacctttag gtaatgagaa
     2401 gaacagactt accattgaaa tcactcccag ccccgccgtt gccagaaatg acatccgcca
     2461 gccgatggtt tcacccaacc aggtcgccgc cggcacgcca ccgatatttg ccagggttaa
     2521 ccccataaac atagtggcaa ctgcgctggc ctgtttatgt tttggcacca cgcttgcggc
     2581 cacgactgaa cccaaaccaa aaaatgctcc gtgattcagg ctggtcaaaa tacgtgaaag
     2641 catcagggtc atataatccg gcgcgatggc ggaaagaacg ttgcctagcg tgaaaattgc
     2701 catcaggaaa atcaacgcac tgcggcgggc acgatgagaa agtagaagcg tcatcagcgg
     2761 cgcgcccacc attacgccaa ctgcataggc actgattaac attccggcag cgggaatcga
     2821 gacatccaca ccgcgcgcaa tgacgggcaa caagcccatt ggcgagaact ccgttgtccc
     2881 gataccaaac gcgccaatcg ccagcgccag caacggatag ttaattttca tgccttatct
     2941 ccacctcttc gcgtcattac gcgatattca ttaaagtggc gaaagcatga cagcaatcac
     3001 aaaaaaatga aaataacaaa aagagaaaac acttttgcca ttttgctaac aaacaggaag
     3061 gagatgcgag ggagaacgcg ctccctcgag aggaaatcag tgcagcgcgg cagtcaaacc
     3121 cacggctacg atcaaaccga ggacgataat cgttgttacc agtgaaaatt taaggtcggt
     3181 gctcatcaag ttttctcctt ttttattacc acacaaaaag tgatattacg catttttaca
     3241 cactgtgatg aaaaaatctc ccgtcattta taatgataag tgtttttacc acttcccctt
     3301 ttcgtcaaga tcggccaaaa ttccacgctt acactatttg cgtactggcc attgacccct
     3361 tcctgacgct ccgtgtcgtt tttccggcgt accgcaacac ttttgttgtg cgtaaggtgt
     3421 gtaaaggcaa acgtttacct tgcgattttg caggagctga agttagggtc tggagtgaaa
     3481 tggaatggca acaataaaag atgtagcgaa acgagcaaac gtttccacta caactgtgtc
     3541 acacgtgatc aacaaaacac gtttcgtcgc tgaagaaacg cgcaacgccg tgtgggcagc
     3601 gattaaagaa ttacactact cccctagcgc ggtggcgcgt agcctgaagg ttaaccacac
     3661 caagtctatc ggtttgctgg cgaccagcag cgaagcggcc tattttgccg agatcattga
     3721 agcagttgaa aaaaattgct tccagaaagg ttacaccctg attctgggta atgcgtggaa
     3781 caatcttgag aaacagcggg cttatctgtc gatgatggcg caaaaacgcg tcgatggtct
     3841 gctggtgatg tgttctgagt acccagagcc gttgctggcg atgctggaag agtatcgcca
     3901 tatcccaatg gtggtgatgg actggggtga agcaaaagct gacttcaccg atgcggtcat
     3961 tgataacgcg ttcgaaggcg gctacatggc cgggcgttat ctgattgaac gcggtcaccg
     4021 cgaaatcggc gtcatccccg gcccgctgga acgtaacacc ggcgcaggcc gccttgccgg
     4081 ttttatgaag gcgatggaag aagcgatgat caaggtgccg gaaagctgga ttgtgcaggg
     4141 tgactttgaa cctgaatccg gttatcgcgc catgcagcaa atcctgtcgc agccgcatcg
     4201 ccctactgcc gtcttctgtg gtggcgatat catggcaatg ggcgcacttt gtgctgctga
     4261 tgaaatgggc ctgcgcgtcc cgcaggatgt ttcgctgatc ggttatgata acgtgcgcaa
     4321 cgcgcgctat tttacgccgg cgctgaccac gatccatcag ccaaaagatt cgctgggtga
     4381 aacagcgttc aacatgctgt tggatcgtat cgtcaacaaa cgtgaagaac cgcagtccat
     4441 tgaagtgcat ccgcgtttga ttgaacgccg ctccgtggct gacggcccgt tccgcgacta
     4501 tcgtcgttaa tcacccgttg cgggagtctc ttccggctcc cgtagccact ccttattcag
     4561 cgtctcacta tcgccgagat actcaagcag ccaggttaac gcaggcgaca tatcattttg
     4621 ctgccatgtc agacaacatg ccgaatccgg gaaggggttt tccagttcaa gtgctaccca
     4681 cttcccctca ttaagccacg gtttggcgaa atgtgttggc accatcccta tgcataatcc
     4741 tgccgagata caggttgccg atgattccca gtcaggtacg acaagtcttt tttggttatc
     4801 cagcaaccag gtaatacgtt taggtagcgt tcgcgaggtg tcttcgcgca ccaacgacgg
     4861 ccagttgcgc aacgtatcat cgctgaacgg gccatccatc aacgccagcg ggtggtgact
     4921 ggcaacaacg caactccagc ttagcatccc catatcccgg aaggcataac gaccgcctac
     4981 cggaatcgcg cgtgttgcgc caattgccag ttccacgcgc ccgtcggaaa gcgcatccca
     5041 gacaccgttg aacacttcct gaaagacaag aagttcgaca tcatcaaaat ggcgataaaa
     5101 atcaacgatc atctgccgtg tacgttctgg cctgacaata ttatccactg cgatagctaa
     5161 ctgaccgcgc cagccgttcg ctatctgctg acattgctgg cgggtgatct gcattttttt
     5221 gacaacagag cgcccttctt tgagaaacca cgctccagca gcggtcagct ccacatcacg
     5281 gtgccgtcgt tcaaagagcg gtaccgccag ccactcttcc agctgacgca cggtatagct
     5341 gaccgcagaa ggaacacgat gcagctcctg tgccgcagcg ctaaaactac cattacgcgc
     5401 taccgcatca acaacttcga gtgaatattc tgaccacata gtctgcctgc aaaatttttg
     5461 aaaccagtca tcaaatatta ccgtttcaca acactaattt cactccctac actttgcggc
     5521 ggtgtttaat tgagagattt agagaatata catgcaacct gggaaaagat ttttagtctg
     5581 gctggcgggt ttgagcgtac tcggttttct ggcaaccgat atgtatctgc ctgctttcgc
     5641 cgccatacag gccgacctgc aaacgcctgc gtctgctgtc agtgccagcc ttagtctgtt
     5701 ccttgccggt tttgccgcag cccagcttct gtgggggcca ctctccgacc gttatggtcg
     5761 taaaccggtg ttattaatcg gcctgacaat ttttgcgtta ggtagtctgg ggatgctgtg
     5821 ggtagaaaac gccgccacgc tgctggtatt gcgttttgta caggctgtgg gggtctgcgc
     5881 cgcggcggtt atctggcaag cattagtgac agattattat ccttcacaga aagtcaaccg
     5941 tatttttgcg accatcatgc cgctggtggg tctgtctccg gcactggctc ccctgttagg
     6001 aagctggctg ctggtccatt tttcctggca ggcgattttc gccaccctgt ttgccattac
     6061 cgtggtgctg attctgccta ttttctggct caaacccacg acgaaggccc gtaacaatag
     6121 tcaggatggt ctgaccttta ccgacctgct acgttctaaa acctatcgcg gcaacgtgct
     6181 gatatacgca gcctgttcag ccagtttttt tgcatggctg accggttcac cgttcatcct
     6241 tagtgaaatg ggctacagcc cggcagttat tggtttaagt tatgtcccgc aaactatcgc
     6301 gtttctgatt ggtggttatg gctgtcgcgc cgcactgcag aaatggcaag gcaagcagtt
     6361 attaccgtgg ttgctggtgc tgtttgctgt cagcgtcatt gcgacctggg ctgcgggctt
     6421 cattagccat gtgtcgctgg tcgaaatcct gatcccattc tgtgtgatgg cgattgccaa
     6481 tggcgcgatc taccctattg ttgtcgccca ggcgctgcgt cccttcccac atgcaaccgg
     6541 tcgcgccgca gcgttgcaga acactctgca actgggtctg tgcttcctcg caagtctggt
     6601 agtttcctgg cttattagta tcagcacgcc attgctcacc accaccagcg tgatgttatc
     6661 aacagtagtg ctggtcgcgc tgggttacat gatgcaacgt tgtgaagaag ttgactgccc
     6721 gaatcatggc aatgccgaag tcgctcatag cgaatcacac tgatctatat cgatatactt
     6781 atacttaggc tgctaacaaa attttgttgt atgattgaaa ttagcggcct atactaattt
     6841 cgagttgtta aagctacgat aaatattatg tttttacggg gacaggatcg ttcccgactc
     6901 actatggatg gtcatttcgg caagggttcc tcctttccct ctgttctacg tcggattata
     6961 gactcgcggt tttttctgcg agatttctca caaagcccaa aaagcgtcta cgctgtttta
     7021 aggttctgat caccgaccag tgatggagaa actatgagtt catcgtgtat agaagaagtc
     7081 agtgtaccgg atgacaactg gtaccgtatc gccaacgaat tacttagccg tgccggtata
     7141 gccattaacg gttctgcccc ggcggatatt cgtgtgaaaa accccgattt ttttaaacgc
     7201 gttctgcagg aaggctcttt ggggttaggc gaaagttata tggatggctg gtgggaatgt
     7261 gaccgactgg atatgttttt tagcaaagtc ttacgcgcag gtctcgagaa ccaactcccc
     7321 catcatttca aagacacgct gcgtattgcc ggcgctcgtc tcttcaatct gcagagtaaa
     7381 aaacgtgcct ggatagtcgg caaggagcat tacgatttgg gtaatgactt gttcagccgc
     7441 atgcttgatc ccttcatgca atattcctgc gcttactgga aagatgccga taatctggaa
     7501 tctgcccagc aggcgaagct caaaatgatt tgtgaaaaat tgcagttaaa accagggatg
     7561 cgcgtactgg atattggctg cggctggggc ggactagcgc actacatggc atctaactat
     7621 gacgtaagcg tggtcggcgt caccatttct gccgaacagc aaaaaatggc tcaggaacgc
     7681 tgtaaaggcc tggatgtcac cattttgctg caagattatc gtgacctgaa cgaccagttt
     7741 gatcgtattg tttctgtggg gatgttcgag cacgtcggac cgaaaaatta cgatacctat
     7801 tttgcggtgg tggatcgtaa tttgaaaccg gaaggtatat tcctgctcca tactatcggt
     7861 tcgaaaaaaa ccgatctgaa tgttgatccc tggattaata aatatatttt tccgaacggt
     7921 tgcctgccct ctgtacgcca gattgctcaa tccagcgaac cccactttgt gatggaagac
     7981 tggcataact tcggtgctga ttacgatact acgttgatgg cgtggtatga acgattcctc
     8041 gccgcatggc cagaaattgc ggataactac agtgaacgct ttaaacggat gtttacctat
     8101 tatctgaatg cctgcgcagg tgctttccgc gcccgtgata ttcagctctg gcaggtggtg
     8161 ttctcacgcg gtgttgaaaa cggccttcga gtcgctcgct aaaggctatt ctatcgcccc
     8221 ctcttcgggg gcgagttcag atcaggcttc tgtacctggt tgattcatgg cattttctcg
     8281 tgccgccagc acacgttcta ccgtatctac cactgcctga gtttgtggat cgatttcaat
     8341 gttgacgcgt gcgccaagtt ttttcttccc aagagtcgtg cgttccagtg tttccggaat
     8401 taaatggacg caaaaacgcg ttggcgtgac ttcgccgacg gtcaggctaa taccgtcgat
     8461 gccaataaat cctttgtaca ggatatattt catcaactga ctatcctgga ctttaaacca
     8521 gatctggcga ttattttccg aggttaatat tttcgccact tcagcggtgg tcataatatg
     8581 acctgacatt aagtgtccgc caatttcatc actgaatttc gccgcacgct caacgtttac
     8641 ccaatccccc acttttaaat cgccaagatt ggtaatgcgt aacgtttctt tcatcaggtc
     8701 aaaactgaca tggttgccgt taatttccgt cacggtcagg cagcaaccgt tatgcgccac
     8761 ggaagcaccg gtttccaggc cgtccagcat gtggtcgggt aactccacca catgcgtacg
     8821 aaaatttggt ttctcgtcaa tcgacaccag ttttgcggtg ccctgtacaa tccccgtaaa
     8881 catacttaca actcctgaaa tcagttaaga cattctgttc agcacaatag caggtggaaa
     8941 acgcccttac cagtgaaggg gtaagaatgg ctattttttc actggagaat taataaatcc
     9001 tcgctacaat agactgaatt tcccctgctt cttctttttg ctgcccattc aggcggcttt
     9061 ttagtctctc atataactac aaataaaagg tgttcacgtg cagaagtata tcagtgaagc
     9121 gcgtctgtta ttagcattag caatcccggt gattctcgcg caaatcgccc aaactgcgat
     9181 gggttttgtc gataccgtga tggcgggcgg ctatagtgcc accgacatgg cggcggtcgc
     9241 tatcggtact tctatctggc ttccggcgat cctctttggt cacggactgc tgctggcatt
     9301 aacgccggtt atcgcgcaat taaatggttc cggtcgacgt gagcgcattg cgcatcaggt
     9361 gcgacaaggt ttctggctgg caggttttgt ttccgttctc attatgctgg tgctgtggaa
     9421 tgcaggttac attatccgct acatggaaaa catcgatccg gctctggcgg acaaagccgt
     9481 gggttatctg cgtgcgttgt tgtggggcgc gccgggatat ctgttcttcc aggttgcccg
     9541 taaccaatgt gaaggtctgg caaaaaccaa gccgggtatg gtaatgggct ttatcggcct
     9601 gctggtgaac atcccggtga actatatctt tatttatggt catttcggta tgcctgagct
     9661 cggtggcgtt ggttgtggcg tggctactgc ggcggtgtat tgggtcatgt tccttgccat
     9721 ggtttcttac attaaacgcg cccgctccat gcgcgatatt cgtaacgaaa aaggcaccgc
     9781 taagcccgag cctgcggtta tgaaacgact gattcaactc ggtttgccga ttgcgctggc
     9841 actgttcttt gaagtgacac tgtttgccgt cgtggctctg ttagtgtctc cgctcggtat
     9901 tgttgatgtc gcaggacacc agattgccct gaactttagt tcactgatgt ttgtgcttcc
     9961 aatgtcgctg gcggcagcgg taactatccg cgtaggttat cgtctgggtc agggctcaac
    10021 gctggatgcg caaaccgctg cgcggaccgg gcttatggtg ggtgtctgta tggcaaccct
    10081 gacggccatt ttcacggttt cactgcggga gcaaatcgcc ctgttgtaca acgacaatcc
    10141 cgaggttgta acgctggctg cgcatttgat gttgctggcg gcggtatatc agatttctga
    10201 ctcaatccag gtgattggca gtgggatttt gcgtggttat aaagatacgc gttccatttt
    10261 ctatatcacc tttacagctt actgggtgct gggcttgcca agcggctata ttctggcact
    10321 gaccgatctg gtcgttgaac ctatggggcc agcaggcttc tggataggct ttattatcgg
    10381 cctgacatcg gcagccatta tgatgatgct gcgtatgcgg ttcctgcaac gtatgccgtc
    10441 agccatcatt ctgcaacgag cgtcccgcta ataaagacaa ggcgcaacct tcacgggttg
    10501 cgcctgtgtt tttacgcagg ctggagcgtt gcgccaatcc cgtcttcgtc tggctgtaat
    10561 ttcagagcgt tacacagagt ttgccgactg gcatctgtta tctcaacgcg tatatccccg
    10621 ttaacttccc ctttcacatt gatgaagtca ggtgaaattt ctttcgcact ggtttgcagg
    10681 caaatcgctc cctctccgcg caggcgtttc actttcagtt gcacatgcgt tgccgcacca
    10741 gtgatcagcc acagttttcc gccattcaga ttgatgttac caacaatgaa agtgacgctg
    10801 tcacctttta ccgtactgcc gtcgagaatc aacacaccgc cctgctgtac agtaatcgtt
    10861 gattgttgca gctctgcgcc ataaccaata atcattgaag cgccgtccag taatgcaaca
    10921 ctcccggtca gttcaaccgg tgcgcgatct tcctctacga caaaattttc gtccgcgctg
    10981 gtgggtgaca acaggatcac tgttccacct tgtattaccg cattttccag cgtaccggca
    11041 tgcacttcag cccgcccccc cacattcacc gacaggtttt gcgtcttacc ggaactgtag
    11101 agctgaaggc catccgtccc cagacgataa atggatccat tctcaacaat agtgtcagag
    11161 tcttcaccac cgagattatt catggtgcca ccagcaagca acgtatcact ggctttcccg
    11221 gccagcataa aaacgccgcc ctgattaatc tcacaattgc tggcttctcc acttggatac
    11281 accaacaatt caccaccttc atctaccacg accgctgagg ttgtcccatt aaccaacagg
    11341 ccgccctctt gatcgagaat gattttttcc gcgcgatgtc cttccagtac acgcaggtta
    11401 ccgccatttt ccagcaacaa accgcaggca taaccctgat caacgctgaa ttcaccttcg
    11461 ggatggcggc cattaaccgt agcgagcgta gagagactaa ttgcgcctcc ctcggactga
    11521 acaatatctg tggcaatggc accgtcgctg accgatagcg tgccattgtt accgtactgt
    11581 gtgcatttgg cgatcccacc ggttgccact ttcaaccagc cagcccccgt gaccacggta
    11641 ttttcggctc ttccgccagc ttcaacaatc aactcgcctt cagtaagttg cgtcgcgcca
    11701 aggacctcgc cggtcttaac aatttgcaca ttcccgtcgc tcatcaagtt tttcgcatca
    11761 gatcccatga tttattcctt tgctgcatct gtgtgccttt attgctacct aagtgtaaag
    11821 gctacggagg atttatccac gacagatttg agatggtggc aaacaactct gtttaaactc
    11881 tgatacacga attattgggt tgtatcagat gtaaatgcga tcctgaataa aaatcgccct
    11941 tgcaaatcaa caaaatatag ccaatttgct taaaagctct gcaaacggtc gaaatagcgc
    12001 agaaaattac gttttgcctc ttgccacctt tccactctgc cgctaatatt cgtccccgtt
    12061 gtcacctaca acgttgcgtt catagctcag ttggttagag caccaccttg acatggtggg
    12121 ggtcgttggt tcgagtccaa ttgaacgcac catcctgcgt ccgtagctca gttggttaga
    12181 gcaccacctt gacatggtgg gggtcggtgg ttcgagtcca ctcggacgca ccagattttc
    12241 ttaatctggt cttttccttt ttccctctgt ttcttctctg tatccaatac gttaaaagat
    12301 ttacactgtc ttcgtatgcg ttatcagaag gagaatcgct atggcaactt tgttacaact
    12361 tcattttgct tttaatggcc catttggcga cgcaatggct gagcagctta aaccacttgc
    12421 tgagtcgatt aatcaggaac ctggttttct gtggaaggta tggacagaaa gtgaaaagaa
    12481 ccacgaagcc ggtgggatct accttttcac tgatgaaaaa agcgcccttg cctatctgga
    12541 aaaacatact gcccgactga aaaatctcgg cgttgaggaa gttgtcgcca aagttttcga
    12601 tgtcaatgag ccacttagtc aaatcaatca ggcaaaactc gcctgacaga atttaatcaa
    12661 gggcggttag cgcccttttc atccctgtct gaaatttctc aaattctaaa aatctcaacc
    12721 aaacttatct gataacacta aattcgaaag aatgcgtaca ggtaagtaac aatgaaaaaa
    12781 attgctattg tgggtgccgg gcctacgggg atctacacct tattctcgct tctacagcaa
    12841 caaactccac tttctatttc tatcttcgag caggctgacg aggccggtgt cgggatgcca
    12901 tacagtgatg aggaaaactc aaaaatgatg ctggcaaata ttgccagtat tgaaataccg
    12961 ccgattaatt gtacgtatct cgaatggcta caaaagcaag aagccagcca tctccagcgt
    13021 tatggcgtta aaaaagaaac cttgcacgat cgtcagtttt taccgcgaat tctgctgggc
    13081 gaatatttcc gcgatcaatt tttacgatta gtagaccagg cacgacagca aaaatttgca
    13141 gtggctgttt atgaatcatg ccaggttacc gatctgcaaa ttacaaatgc tggcgtcatg
    13201 ctcgctacaa atcaggattt acccagcgag acgtttgatt tagcggtgat tgccacgggt
    13261 cacgtctggc ctgatgaaga agaagcaacc agaacgtatt ttcccagccc gtggtcaggc
    13321 ctgatggaag caaaggtcga tgcgtgtaac gtgggtatta tgggaacatc cttgagcgga
    13381 ctggatgcgg caatggcagt ggctattcag catggttcgt tcattgaaga tgataaacaa
    13441 cacgtcgttt ttcaccgcga taacgcaagt gaaaagctaa atattacgtt gatgtcgcgc
    13501 acgggtattt tacccgaagc cgatttctat tgccctattc cctacgagcc cttacacatc
    13561 gtcaccgatc aggcattaaa tgctgagatt caaaaaggtg aagagggcct tttggatcgg
    13621 gtatttagat tgatagtaga ggaaatcaag tttgctgatc cagactggag ccaacgcata
    13681 gccttagaga gcctgaatgt cgattccttt gctcaagcct ggtttgccga gcgcaaacaa
    13741 cgcgacccat ttgactgggc agaaaaaaat ctccaggaag tcgaacgcaa taaacgagaa
    13801 aaacatactg ttccctggcg ttatgtcatt ctgcgcctgc atgaagccgt acaggaaatt
    13861 gttcctcatc tgaatgaaca cgaccataaa cggttcagta aaggccttgc cagggttttc
    13921 atcgataatt atgcggcaat cccttcagag tctattcgtc gactactggc cttacgtgaa
    13981 gcggggatca ttcatattct cgccctcggt gaagactacg aaatggaaat taatgagtcg
    14041 cgcaccgtcc tgaaaacgga agacaacagc tactcgtttg acgtttttat tgatgcccgc
    14101 ggacagcgtc cgcttaaagt gaaagatatt cctttccctg ggctacgcga gcaattacag
    14161 aaaacagggg atgaaatccc tgatgttggc gaagattata cgttacagca acccgaagat
    14221 attcgtggac gcgtagcgtt cggcgcgttg ccctggttga tgcacgacca gcctttcgtt
    14281 cagggactta cggcatgtgc agaaattggt gaggcgatgg ctcgggcggt cgtaaagcct
    14341 gcatcccgtg ctcgtcggcg tctttcgttt gattaaaggt aaagctatca gcactcaaca
    14401 aactgatgct gttcattaag acgatagcgg actccggttc gaatattgtt ttcaccttca
    14461 atggcgacgg cgaagcgaac tctttcgccg tcatgatatg ccagtgcggc gcttccccct
    14521 agcccgagaa taattgagtc gaccacgccc gtgctcacta ccgttgaatt ttcaccgctg
    14581 gcaatgatgt gtacattgtc gccgctgttg gcaattctgg cgtttgcgcc aaagctggcg
    14641 atttgcacca ggtcaccatt actggcgaca tgacaccgtt cacccaatgt acaaacgcgt
    14701 actcgcattc cggtattcgc aatccgacta ctatctccag cgctgcttat ccgcgaagaa
    14761 ttaccggtta cagcaacccg tgcgcgttca ccggagctgc caatgtgact gttaaagccg
    14821 acacttccaa tccgcacgct ataacccata ctggcaattt gcgcggcata cccagcggtt
    14881 gcgattcggg cgttatcctc acaacagccg atgcgagcat gatcgtcgct gcgggcaaac
    14941 tgactgcaaa atggtatctc tccggggtct gtggccagtt tttgcataga ggaaacttcc
    15001 tgatgcgcga agttttcatc cgccagccat tttttccacg catactcaac cagactctcc
    15061 gcccagtccg tatatccttc ctcaatcagt gcatgacgaa tatcggcata actaccgccg
    15121 cgtgggaaat ggcgcaggaa ccagcgatac atcaccgctc cgatacgcca ttctcgcagg
    15181 tctgctcgtg taatgatcat tcgttagtgc cggtgatagc catcgaccat gcttttaaag
    15241 gtttcgtgtg gcgtacgccc cgtggtgcaa agataaagat gaccgaagat aaaaaagagg
    15301 cttataaatg ccagagcaaa atgcgcctgc aataaccagt atcttacgcc aggaaacaca
    15361 tctcccacgg cttgcggata gagacacaac agccccgtca atagtaacaa cggcagcaat
    15421 ccatacatga caccaacata ggcgacctgc tgtaagggat taaatttaga ctgtgttgtt
    15481 gccgggaaag gatgttcttc cccctgcata atgccaaaca aataaaatcg cgtttgtttt
    15541 gccgctcgtt ccagccaccc ctgacgacga atgcgatagt ggtgaccatt atccccaacg
    15601 gcattgatca gcacaaagcc gagccagcat gccagtaaca aaaatccgca aacttcatgc
    15661 accgcaacca gacttttaac cgcagttgcg cccaccatcg caaagtgatt tatcagccca
    15721 cttgccagca acaatacaaa gagtaacgca ttcgaccagt gccataatct gaccgctttg
    15781 ctgtaaagat aaaccttctc accatgatcg gtggctgatt tcttcacgcc acgagcacga
    15841 agcaaggcgt gcagccccag caccaaccac atcccaacca gcaatactcc ggcaatgatc
    15901 aaccacaccg gccaaaattc gggagtgaac tgtggcacat agttcgctaa ctggctctga
    15961 aactgttcag catgttgcga cgggttcata cattttcctt tttaatcaaa tgttgaccga
    16021 accggcgata cagatgcggt tttccggcac caggtagctg atattgatag tatttattat
    16081 cctgcaacca cgcctgaatt tctgggctat cttcgcgacc aaagatcaat gcgtgttccg
    16141 gacaggcgct gacgcaaatg ggcggaaagc cttttgccag tcgggactca gcgcagaaat
    16201 cacatttatc tgccacttta gtgacagggt taaggtagcg aacctggtac ggacaagcgc
    16261 caatgcaata actgcagccg atgcactgcg atttttctac ccgcacgatc ccctgctcat
    16321 cacgccacga tgcccccgtc gggcatacgt cgatgcacgg agcatcttcg cagtgctgac
    16381 atgactggcg aaaaaagtga tactgcgttt cgttgtcgtt atcggtgact ggaatgtgtg
    16441 ctatcgataa acggcttccc tgagcgggga cgtggttagt tttacggcat gcgcgggcgc
    16501 aaatattaca gccattgcac cgtgactcgt catgaatcat ggcataccga acttcctttt
    16561 cttgcctcgt gttcgctaac agagaagacg cagaaccagt aaaaaagatt actgttccca
    16621 tccccagaac aaatttgcgt cgagtaaagg acatctgatt atcccctaat gcagtggctg
    16681 caactgtcca ccctatccat gacatctccc tgaaatttca ggggttctga aactgctcta
    16741 cctcgaacca ttcgcataag ccataagcct tgccataggt cataaatcgc tcgctaaaca
    16801 agtacaacac cgccgtcgat gactccacct gccgtatatc ggcatattca ggtgcaacat
    16861 ccatcgcggc gatcgcggct tcaatctgcg cttcctggaa gtaatatggc gcttgcatca
    16921 gcatggcgac tttgtaagga cgaggatacg tctggcactc aaaacgaacg gcgtgtgcaa
    16981 tggcgcgaca aatgtcctgc tccacaactt gcaaagacat cgccgcgtag ttttcactca
    17041 tggcctgcgt tgagtaatag taatcatcct gacttcccgt catacaagcg atatccgcgt
    17101 agtctccgtt ctgcttaatg ctttccagca aaactgacag ttcctcttct gcaaccgaat
    17161 agggtggcga gagaaacacg gtgcgcgcca ccagctgccc agccgcagaa tgctggcgaa
    17221 tgaactcagc caggatttca gcaggttctg gttcggttac cgtatcaacg gggtgacgca
    17281 tcccctgcaa ccattgccgc ttcgcctcgt caacttcgct gactttcgct aacggcaatt
    17341 catcctgatg gttcatctta cccattcctt acgcaggcag tagattgtgt gctgccagat
    17401 cggcggcaat atcttccagc cccagacgct gcaatgtttc gcgggttgga caaccaagct
    17461 gtgggtccca gcccatttct ttgtagaaca tcgtgagcga agcatgcata tcgtcacgat
    17521 ccattttgtc agtaccttca gtaaagaccg ggatctgcgg atccttgtcg aaaacccagg
    17581 aacagataag atcgtgttca ttacgcatat ctttggtttg catcggtttt accgtgtagg
    17641 cacgatgcaa cgtaaagata cgctctgcag ctaaatctaa tttttcctga gtcatctctt
    17701 cgccggtgat cgctttgaag aatttggctt aaagcgccag atcgccccgg taattacggc
    17761 ttttcagcgg cgaaacggtc attggccaga cccagttgca cagagtgacg gcgttatgca
    17821 aacagacccg caacagcgac cactttgcat atttaatttt tgcgtcattg attggcgtgt
    17881 agtttttggt ttcatcgtaa gcatcttcag aaccaaaaag ttctttcgcc acttcacgtt
    17941 gcagtttcaa tggcaagccg gaaccaataa agttgatatg ggtatgcgtc atgcagtcac
    18001 ggttgaacat acagttaaca atggaaccga cctgcgctga cgcttcattg gcatggtgaa
    18061 ccggatagcc aaacggcgac cagagtttat ttttcgcgta gccccagtac tctttaccca
    18121 aattccagcg ttctgcgatg gcatatgaac catcagccag gtgactcagc tcacccacac
    18181 gatgcgccag acggtagtaa aaatctttaa tgaagttaac gtcacccgct tccagttgat
    18241 cccagcgaat ttctgcatac tcttcagctg gcagaacacg cttgaacaca cctttgctgt
    18301 aacaataagt aaagtcgcga tgcaactgcc cgtagttaca ccataggccg tagtcatcga
    18361 acaaattcag accaaccagg ttaccaatca cacgaccatc gtctttatct tcaaaatctt
    18421 tcggcccgtt cgggaagatg gtggtatgga caaagtttgc tacacaagtg ttaccacctg
    18481 tgctgggcac gccaaactct ttcacccgag gaatattcat ttgggtcata caacggatcg
    18541 ggcaagagtg gcagccgctc atttttactg tgtatttctc tgccgccggt cctaagtcaa
    18601 aaacggattt ataggtacga aagccgaccg tattctgatt gcctggcgga atttcacccg
    18661 tttcaatcgg accaccttca gccgcgcccc aaaacagccc tttacgtgct gtccagcgtg
    18721 acttgggatc tgaatactct gcccacgatt gtggcgtact tggcacgaca tggttgttat
    18781 tcgcaccaat aagttcagtc atcatgtaat cattgagacg cttcatctcc tgacgatcgg
    18841 caatgttgac ccctttcgtc ccttcaaccg caatcgcttt caggtttttc gaacccatta
    18901 ttgcgccagt tcccgcaccg ccgctgtggt tacggctatt caacatgcca gagagaggaa
    18961 taaggttttc cccagcctga ccaatagccg ccacacaggt ttccgggctg gtcaatcgac
    19021 aaatttcttc cgtcgttgcg cgcgtccctt ttccccataa gaaatcggct ttttccaggc
    19081 taactttgtc atctttaatc ttcagccata ccggtgattt cgccttccct tcgataataa
    19141 tgacgtcgta tccagcgaat ttcatttgcg ctgcaaaaaa gccacccata tgggcatcgc
    19201 gactaaattt cctttggtaa aagtagaaag tgaggtgata tttacgcgag aactacaggg
    19261 ggcaccagat ccagttaatg ggccggtagc aaagactaat ttattcgctt catcgaaagg
    19321 tttcgtgcct agcggtactt cgtcatacat aattttgtag ccgaagccca tgccaccgac
    19381 aaaactttta aacttactgg aatcttcgag ggtaatattt cctgtcgtga gattgactct
    19441 taatatatta cctgtccaac cgttagccat gattttttcc tttgcaagat tcacacagta
    19501 atatctttcc actcgataat ttttaacgcc cctgttgggc aggcgtttgc gcattcaccg
    19561 cataacacac atttcgagga ttttttactt tcggtattta cggtggccat catccacggg
    19621 catgccgtag tacaggcgct acagccaata caacgtttat gatcgacggt aatacagcct
    19681 tctttctgct gccaggtaat cgcaccaatc gggcagacgt tcatgcattg cggctctttg
    19741 cattgacggc aggtgtccgc ggtatagttg agatcgccat acaggccgcc gccagagcca
    19801 accccgttgt cgccaaagaa ataattgcga tggattttga tacgggagaa gaatgtccct
    19861 actgagccat cgttgaagtt ggtacaagag atttcacagc ggtggcaacc cgtgcatcgc
    19921 gctcgctggg tcaccagcac ccctttgggt gtatttatca accctactgt gccgctatca
    19981 atatcttcct gtttgcaacc caaaagtgac agtaacgcag gggcaattgt tagaccagca
    20041 aggccttttc ctgatatacg caagaattct aatcgcgtta agccaatatc taatagtgga
    20101 cgatcaaccg ggttcattta tttcgcatcc tcttcgcaaa cacagtgctg acaattcagc
    20161 agcctgatat taagcattca actaatacgc tgctgactct gtccccaaca ccagatattt
    20221 tccctaaccc gatagtgtat gagaaaagat ttaacgccga taaactaatc ctttatggtt
    20281 aggagaaaaa ataatgtgat tattctgagc ccttaacatt gatcgttatc aattaaaatt
    20341 acaaagcaag aaattataag cgaactgata tttattatca tttgaaataa atttaactta
    20401 agcgacaatc atttgtgacg ttcattgcag aaaataaaaa taacattatc gctataaatt
    20461 aaaatacata ccgaaattcg tataatttca gcagttgtta tttagaatga ttaactggac
    20521 cgcaactgaa gcaagaatat caggatgaag cttaccgcct catcctgaat ggtcatttta
    20581 ccagtgcgtt aatgacggtt tccatcgcct gcttttccag ctcactgcgc tgaatgcgtt
    20641 tattggctaa tgctgtacgt aatacgcctg cgataacaat atgctttggt tcctgcccca
    20701 gatcgcgcat ttccagcaca actttaccga caactctgca catttctcgg tacagttcat
    20761 cctcttttgt ccgatttccc attctttata cctatttatc atgccaacta
//
LOCUS       DAJTQY010000067        20713 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.067, whole genome shotgun sequence.
ACCESSION   DAJTQY010000067 DAJTQY010000000
VERSION     DAJTQY010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20713
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.067"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(76..4536)
                     /gene="mukB"
                     /locus_tag="OO349_002614"
     CDS             complement(76..4536)
                     /gene="mukB"
                     /locus_tag="OO349_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HCR5867637.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEVDLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFSS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLLGRDEAPSQAS"
     gene            complement(4536..5240)
                     /gene="mukE"
                     /locus_tag="OO349_002615"
     CDS             complement(4536..5240)
                     /gene="mukE"
                     /locus_tag="OO349_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HCR5867638.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEESQPDSGEEE"
     gene            complement(5221..6543)
                     /gene="mukF"
                     /locus_tag="OO349_002616"
     CDS             complement(5221..6543)
                     /gene="mukF"
                     /locus_tag="OO349_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HCR5867639.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6540..7325)
                     /gene="cmoM"
                     /locus_tag="OO349_002617"
     CDS             complement(6540..7325)
                     /gene="cmoM"
                     /locus_tag="OO349_002617"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HCR5867640.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     YQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7461..8240
                     /gene="elyC"
                     /locus_tag="OO349_002618"
     CDS             7461..8240
                     /gene="elyC"
                     /locus_tag="OO349_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HCR5867641.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(8217..9110)
                     /locus_tag="OO349_002619"
     CDS             complement(8217..9110)
                     /locus_tag="OO349_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HCR5867642.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9264..10010)
                     /gene="kdsB"
                     /locus_tag="OO349_002620"
     CDS             complement(9264..10010)
                     /gene="kdsB"
                     /locus_tag="OO349_002620"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HCR5867643.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHDDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(10007..10189)
                     /gene="ycaR"
                     /locus_tag="OO349_002621"
     CDS             complement(10007..10189)
                     /gene="ycaR"
                     /locus_tag="OO349_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HCR5867644.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10241..11473)
                     /locus_tag="OO349_002622"
     CDS             complement(10241..11473)
                     /locus_tag="OO349_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaQ family DNA glycosylase"
                     /protein_id="HCR5867645.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11510..12496)
                     /gene="lpxK"
                     /locus_tag="OO349_002623"
     CDS             complement(11510..12496)
                     /gene="lpxK"
                     /locus_tag="OO349_002623"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HCR5867646.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(12493..14241)
                     /gene="msbA"
                     /locus_tag="OO349_002624"
     CDS             complement(12493..14241)
                     /gene="msbA"
                     /locus_tag="OO349_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HCR5867647.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DNLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14278..16537)
                     /locus_tag="OO349_002625"
                     /pseudo
     CDS             complement(14278..16537)
                     /locus_tag="OO349_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415433.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
     gene            complement(16744..17028)
                     /gene="ihfB"
                     /locus_tag="OO349_002626"
     CDS             complement(16744..17028)
                     /gene="ihfB"
                     /locus_tag="OO349_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HCR5867648.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(17188..18861)
                     /gene="rpsA"
                     /locus_tag="OO349_002627"
     CDS             complement(17188..18861)
                     /gene="rpsA"
                     /locus_tag="OO349_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HCR5867649.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(18972..19655)
                     /gene="cmk"
                     /locus_tag="OO349_002628"
     CDS             complement(18972..19655)
                     /gene="cmk"
                     /locus_tag="OO349_002628"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HCR5867650.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQLIEKALQ
                     YARQKLALA"
     gene            complement(19832..20593)
                     /gene="ycaL"
                     /locus_tag="OO349_002629"
     CDS             complement(19832..20593)
                     /gene="ycaL"
                     /locus_tag="OO349_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HCR5867651.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASG"
BASE COUNT         4984 a         5725 c         5031 g         4973 t
ORIGIN      
        1 cgttatgtgc cattttttta gtgcagaaac ggagttttcg gaaaaagaaa aggcggcatt
       61 gctgccgcct taattttaac tcgcctggga aggcgcttcg tctcttccta gaagcgtttc
      121 agggagttgc ggcgcaaatc ctcgcaggcc gacgacgtga acatgttcgg tattctggaa
      181 gactttacgt accagtttat aggtggtgcc tttttccgga ctgatatttt ccggcgctgc
      241 gatgatgagc tgcatttgca aacgttcaca caattcaaac agcgtggcga tagaacgagc
      301 atccagtcgc gctgcttcat cgaggaacag caggcggcaa ggagagatat ctttaccgcg
      361 caggcggcga gattcatctt cccagctttg taccaccatc accagaatcg acataccggt
      421 accaatcgcc tcaccggtcg acaatgcacc agactctgcg cgcaaccaac catcggaacc
      481 acggttaacc tcaacttcca tttccagata gttgcggtaa tccagcagtt cttcgccaat
      541 ggtctgcggc gtgcgctgcc ccatatcaat ctgcggatta agacgctgat acaatttcgc
      601 caacgcttcc gagaaggtca gacggttgct gttaaacaga tcctgatgct gctcgtgctg
      661 ttcagagagc acatccagca gcatggcgtg cgtttcacgc acgttcacgt tgagacgcac
      721 gctgttcacc tgaccaaacg atacgttctg caacccctgg ttgagcatac ggatacggtt
      781 ctgctcgcgc tgaatggttt tgcgaatgat gttcgccacg ctgcgggaac tgatcgccag
      841 tttctgttca cgggaggtta attcttcggt cagacggcta agttcaatct ccatctgttc
      901 gatagcttcc accggatcat cggtacgaat aatatcctga cgaatacgtt cacgcagatg
      961 ctgataaacc gccacgaaga actgaatttt acgctccgga cgtttcggat cttccgacat
     1021 gcgcagcacg tcgcgcagat gttcgttatc cgccaccgcc agacgcagcg cacctaacgc
     1081 cttatccgac atggaacgca aatcatcagc ggagagataa gccagctcac gacggtgtaa
     1141 gcggcgctca acgccgttat ctttcaccat gcgcatcacc gcacaccagc ccgctttggc
     1201 ggtcactacc tgctcgcgca tctcaaagta atcccgctcc agcttgcgca gtttgcgggt
     1261 caggttgtcc atctccgctt cgcagaaggt aagcgctttt tccagttgat tgcggcgtga
     1321 acggttattg ctcagttgcg catgcagctc gtcacggcga atacgcgccc gctcttctgc
     1381 cccgctatca gcacgcacgc cgatatcctg caattcacgt tgcagatcgt tgagtagctc
     1441 ttttttggtg tcgtaagaac ttttcagcga agccagcacc tggttgtact gactcaactg
     1501 cgctgcgtga ccgcgcaacg cttcgcgagc gcgggtacgc tccgcttccg cctgttccag
     1561 acgttcacgc agtttttcgt tgagatcgct gttaccgcta agcatttctg ccgagtcaga
     1621 atagctaaag tgcgcacgac gctgcaccac ttccgtcagg gcaaacgctt gctgacgggc
     1681 atcgcgctgc atctgctgag agtacgcgta atcttctttt aactgttcga actgttccgg
     1741 gtcgctctgc aataccgaaa cgatcggttc cagtttcgcc agttgattgc caaactgctg
     1801 aacaaaacgc gcggcttcct gggcttcatc cagacgttcg cggatttcat cgacgcgatc
     1861 cgccaggctg tcatcagcca acaggttgag acgcggcaga atgcggttca gcgccgtaac
     1921 gccttctttc gcctgctcaa actgaatacg ctgctgctgg ttatcatttt catgattact
     1981 caacgcccgc tccagttcga ctcgacggct gttcagttga cggatttctg cttccgggtc
     2041 agactcaaac gccaccgcca gatgactgcc gataaagcgg ctgaacgcct gatgcagacg
     2101 ctgggttttc tgtacatcaa aggagagcgt ggcgaagcgt tcggaaagca cttcacgctc
     2161 ggcatggagg ctttcaatac ggctttcacg cgcagcacga ccaaacagcg gcacttccgg
     2221 gaaacgtgaa taacgccact gtcgatcggc gattttcacc accaccgctt tttccagctc
     2281 atcaacgctg aacacgctat catcgaatga ctgcggatcc ccttcgatca aatagagatc
     2341 ttccgggcaa tcggtcaagc cttccaggtg ttcggttacc tgtgacagat ctggcaccac
     2401 gatggcgtgg cgtgacgggc catacagagc cgagaagtac ggcgcatctt ccaggctaac
     2461 gtcgtcataa atttctgaca gcagcacacc accaaaacgc tccgccagcg cgttcagacg
     2521 ctgatcttca gagccgccag gctggcttaa acgttcgatc tcttcatcga cggcgttttt
     2581 gcgcgcgcct acttcatcgc gttcaacaat cgcctcgcgc tcacgctcca gcaactgttg
     2641 cagatattca gtgacgtcct ggctggagga gaactcttcg ccgcactgtt cactcaactg
     2701 gttgagactg ttttgcgctg ccagccaaac cggtgcacgc tgcatcaaac tctgaatgcg
     2761 agactgcagc tgttcctgtt cctgccgcag tgccatccgc tcttcacggg cgttagatac
     2821 gctatcggaa agagaggcaa tgcgtgcttc cagctcctga tgcagagctt ccagttcatc
     2881 aatatcaaaa ttcttgccct gacgtttgca gaaatctgcc agcagacgct cagcttcttg
     2941 ctgctcacgc agacgctgtt ccagttcgct taagcgcatc cgcaacggct gaacctgctc
     3001 tgccaggtga cgctgatcaa ccccttcgcg caatagttcg cgagcgacat cccacgcctc
     3061 gttacgcgcc agcgggccgt tgattgccac caccagctga taagcctgct caaactggct
     3121 gtgcgcggtt tgcgccatgc tcattttctg ctcaagagag agcatttttt cagtcgcttc
     3181 cagctctttc gcctggaagg tttccagcca ttcggcggcg ctgtcggcgg ttaagtccgg
     3241 cagatggcac agttctttgg cacgattaag cgcagcaatc gcctggttat actggatcgc
     3301 gcgcgtttgc tggacgtcca gcgcctgctg gtagtcagca agctggcttt tcagctcgtc
     3361 cacttccagt tcggcagctt ccgcgcgagc ctcattctct tcctggcgtt cgatggcttc
     3421 tgccaccact tcattttgct cttccagacg gatctgcagc tcatcgagat ccacttcgta
     3481 gcgttcgatc ttctcctgct gacgcaatgc ggtttgcacc aggttcaggt gatcgctggc
     3541 cgcctgataa tccgcttcca gatcgccttc ggcaccgttg tgctctgcca gctcacgcgc
     3601 catatcgacg tgtttgtact gctcagccgc cagttgctga cgcgaagtat gtagctcgcg
     3661 acgaaactcc agggctttgt cgagatggac acggcgctcg ttggcgtgac gcatgtagtc
     3721 cgccgccacg tagttggtag cttcgctgat cagatgctta aacaggtcac ggtccgactg
     3781 ggtgacacga atcgcttcca gcgtcatgcg gttttcacgc agcgccgctt ccatgtcctg
     3841 gaacgcttta cgcacgccgc tgttttccgg caacaggtag tcgcgcagag aacgggtaat
     3901 ggcactggag atcccgccat acagcgaagc ttcgatcaga cgatagaatt tgctacggtc
     3961 agatgcggag cgcagacgac gcgcgatgat gcccagatcg aacatcagcg agtggtaatc
     4021 ggtaatggag ttgaactgtt taaactgcac gccttccatc gcctcgagct tgtctttcag
     4081 ctcgttaagc ggcagcacgc gcgcctggcg ttcgttcagg gtttcggtca ccagctgtgt
     4141 cggctgcacc gacatcggca gtccctgaat ggcaaatggt ttgatatcca ctttacgatc
     4201 gcgtccggca acctgttgca gacgcacacc gaccaccacg cgctggtggc gcgagttaat
     4261 ggtgtcgagc atcgaataac agacacccgc tttcagctta ccgtgcagac ctttatcgcg
     4321 cgaaccgctg gtggccccgg cttccgtagt gttacggaaa tgcagcaggg tcaggtcggg
     4381 gatcagcgcc gtaacgaacg ccgccatcgt ggtggattta cccgccccgt taccgccgga
     4441 aagcgtcgtg accagctcgt caaggtcaaa agttcgggca aaaaagccgt tccagttaat
     4501 cagcgtcagt gagcgaaatt taccgcgttc aatcattatt cttcctctcc gctatctggc
     4561 tgactctctt cggtttcatc gttgagttgc agatgatttt caatcggcat tgcttcgcca
     4621 tcacgaatca ggcgacgctg tgcttcacgg ggatcgtcgc cagcacgcac atcggctccg
     4681 aagcggaaca ccgattcggt aatgcggaac ttgctgctgt cgtggcccat aaaccacacc
     4741 atgcctaaac gacgcagacg gttgagcgaa gaacgtactt tctcctgcaa cttctgacgg
     4801 tcaacgtctg aaccggttga acggttgttc accagtttca gcagtttcgc ttcatcggcc
     4861 agggtgagca gttcgtcgta cagttcctgc tgggtgaaaa tcccctcatt cgccagccgt
     4921 tccgggctga gatagagata acagaggatt ttcccgacca tcatatccag ttccgacaag
     4981 acggaacgcg ggataagagt ggtggaacgt gggcgtaaat agaagaaccc ttccggtgcg
     5041 cgaataagct caacgttata acgcgcgtaa aactcttcca gatattcctg aaaatccatc
     5101 aggaatgcat gattatccag ttcgtcgagg ccaatatggc ggcctgaacg taaggcgctg
     5161 tccagcgccg gaaataacgg attcgccagc gcctgcgcca gcttaaccgg catcacttgt
     5221 tcaatatttg tcgatgacat gcgcctgtac cttggctccg taatcattaa tcggctgcca
     5281 tttcgctggc agtccggtga aatctgcttg cgctacgcca agacgtaccg cctgatcaat
     5341 aacaatacgc gcaacgtcaa agtgacgtgc acgcggatac tgtgacagat attcgcgtac
     5401 caccagacca agatccagcg gcacttgtct ggttttgtac acggcaagtt gttcttcgat
     5461 gatcgccgcc agctgttcgc ggatctcgtt aaactcttcg tattccagat cctcaggaag
     5521 ttccccagtc acttcttcat cgcgcagtgc catctcttcg tcacgcatat ccagcagacg
     5581 atcggcattg gcataagtta gcgcccacgg ctcatcaaaa taggtttgta ccgactgacg
     5641 taaccgctga gcaaagacgc ggtttttatc catatcgatc gcggtacgaa taaatttgtg
     5701 tacgtggcgg tcgtagccaa tccacaagtc gatggattgc tggccccaac tgataatacg
     5761 atcgagtttg ctctgcagat cgaacaccag acgatcgacg aaatgcagat cgtcatgggt
     5821 catcgtcgca tcctgaatgc gcaacagatt agcctgcaat ttgtcgcctg ccgcttccag
     5881 cgtatcctgc aattcacgca gcgttccgga agtttccgaa agcaacaatt cacagctgga
     5941 aatcgccgcc cgccagtctt tgttcagcaa ctgggcgata tcgtccttca cctgctgctg
     6001 ctgttcgtcc atcagacgtt gcgtcaggtc gatactgtcg aaaatttctg ctaccgaata
     6061 tttcagtggc gcatagacat tacggtgcca gtgaaattca tcaccgccct cttcggcggc
     6121 atctgctgcg cgtttgagct cacccgccac aatcgacaac tgcatagaaa gacgcagcgt
     6181 agaaaactcg cgctgacgga tgtagtagtc agtaatgccg atgccgagcg gcgtcagacg
     6241 gtaaattgcg ttcccttccg cctgctcgct ggtaaagcgg ttcagcagac gttgacgcac
     6301 catgtcgttg atcgcgttat tggcgcgcac gccgatggtt tcgctggttt gctcaaacgc
     6361 atcactcaca tggcggaatg catccaccag ctcgccttca ctcatctcac catccagacg
     6421 ctcgccgttc agcgtggcaa ccgccagcag aaaagagagt cggtctaccg gcagcgagat
     6481 ggagaagtca ttttttctgg cccaggcaac cagttcgggg actgtctggg aaaattcact
     6541 catactttat ccttgctctg cggtttgcgc gcggtgacat gaatataacg ccctagggta
     6601 atatacggtt cctgacggca ataacgcgtt tctaattcaa gtaatgcttc atagcagtcg
     6661 cgctgctggt acttctcgcg cagataatca tgaaacacgc gaacgcctgt cttacccata
     6721 atttgccaac cagcttcttc cagccacaga taaacctgcg ccgggtcgcg tggataatct
     6781 ggcgaaagcg tccgtttttt ctttttcggc attcccgcct gcacgtaatc aaaattcccg
     6841 gcgaccatgt tatgcatcaa caaaccatgc gcattgtaga acattaacga caacactccg
     6901 cctggacgca acactgacca cagggtctgc aatacactgc ggggatcggc cacccactcg
     6961 agcaccgcat ggaacaatat cagatcaacg ggcgtttcca aatgcgaagc aacatcctga
     7021 gcggcgcaat gtataaattg catgttgtcg ctcacacctt ttgcttctgc cgcctgtttt
     7081 gcgcggtcga tcatctgcgc agaaagatcg cataaaataa cctgatgccc gcgctcggcc
     7141 attttgattg cggtctgccc ttctccgccg ccagcatcca gcacacgcag tttttgcggg
     7201 cccatttccg ccagcacgcg atcgagatcc tgccacagaa tagcctgtcg aagctgccct
     7261 ttggtggtgc cgtaaatgtt acgggaaaac ttttccgcaa tatcatcaaa attgcgatcc
     7321 tgcattgctt tctccaccgg ttggctaaga cgttttctga taaagacgct attttgtcac
     7381 agccacggcg agaatgaacc cgtctggtgt aatatcgcta gcgatccctt gctatatggt
     7441 taaaaaagga accagaaagg atgcttttta cactgaaaaa agtgattggc aatatgttgc
     7501 tgccacttcc gctgatgttg ttaattattg gcgctggcct ggcgctgctg tggtttagcc
     7561 gttttcaaaa aaccgggaag atttttatta gcattgggtg gctggcactc ttgttattga
     7621 gtttgcaacc ggtagccgac cgcctgctgc gtcccatcga aagcacctat ccgacatgga
     7681 ataattcgca aaaagtggac tatatcgtgg tgctcggagg tggttatacc tggaacccgc
     7741 agtgggcacc gagctctaat ttaatcaata acagtctgcc acgcctgaat gagggtatcc
     7801 gtttatggcg cgaaaatccg ggttcaaaac tgatcttcac gggaggcgta gcaaaaacca
     7861 atacggtgag tacagcggaa gtaggtgcca gagttgcgca atcgctgggc gtaccgcgcg
     7921 agcaaattat caccctggat ttgccaaaag ataccgaaga agaagctgcg gcagtgaaac
     7981 aggcgattgg tgacgcccct ttcctgctgg tgacctccgc atcacatctg ccgcgggcga
     8041 tgatcttttt tcagcaggaa gggttaaatc cgctaccagc cccggcgaat cagttggcca
     8101 tcgactcgcc gcttaatccg tgggaacggg caatcccttc tccggtctgg ctgatgcata
     8161 gcgatcgtgt tggctatgaa acgcttggcc gcatctggca gtggctgaaa ggtccgtcag
     8221 gcgagccacg gcaagagtga ttttgatgcc agatcgaagt tgcgccgact aaatcgtccg
     8281 gtattaacca gttgcgcaac ttcatcccat aacacataca accaacgccg ccagatgaac
     8341 gactccgcca ctggcgcgcg ttgcaggtaa ctccagagca aatcttccgc cagagaatta
     8401 tccattagtc ggaacagttc gtattctcgc ggtgcccaaa gcattagtcc cggcccgacc
     8461 atcgccagta actgatcgct gcgcgaatct ttcagcatgc tgcgtaaaca gaagttaccg
     8521 tgaatcaaca cacaattgtc gttaaagcct tcaaataatg ccgggagaca ttcgcgagtg
     8581 cgaaacagga tccgcttatc ctgcatcgtc agaccggtgt tattgaactg attgagcgtg
     8641 gtccatagca cttcaacatg ttgccggtac catgagggcc agaaattttc ctgagtattg
     8701 tcgaccgcgc cgacgcaacc gcgactgtcc tgacggtgcc aggccagtaa ggcttcaacg
     8761 atttggtctt tgagctgttc ccagcgttct ggtgttcggg ctggtgcctc caccgaaaca
     8821 ccacgcatcc gctccagtag caggacatca gggccggggt gttcttcatg cgtcatcaca
     8881 ccataaatag tcggcatacg gacagtccca cttcttgcca gcatggtggt tttccatgcc
     8941 agttgtcggg cttttccggg cgtactaaag cttcttgcca ttaacggcat tgggtttccc
     9001 tggctgtcat acaaagccca caacgccgta tccgcttttt cattgacgca ctcaatacga
     9061 ctgagttttt cgcccagtaa atggcttaat tcggcacgca gctgttccat tcagattacc
     9121 ctcataaaag ctactgcttt cataatgagc gccaatataa aagatgtcac ctgaatgaga
     9181 tcaattgttg aaaagaaaaa ttgaggaaaa tcgtaacggt acgacactcc tcccaaaatt
     9241 ggctgaagtg tcgtgaagtg aaattagcgc atttcagcgc gaacgcgctc aagatcttca
     9301 ggggtatcca cacctgtgcc aggaacttcc tgagcaacag caacatggat tttttcgccg
     9361 taccacagaa cacgaagctg ctctaacatt tcgatgtgtt ctaacggact tgcctgccag
     9421 ttgacgtaac gacggataaa gcctgcacgg tagccataaa taccaagatg acgcaggaag
     9481 ttatcgccaa cggtttcaag gccttctgca aaacgatcac gatcccaagg aatggtggca
     9541 cgtgagaagt acagtgcata cccttcagcg tcgagaacca ctttcaccgc attcgggtta
     9601 aacgcttctt ccgcattgtg gattggcacc gccagagtcg ccatacccac ctgacgctga
     9661 gcgaggttat cagcaacctg acgaatgatt gtcgcaggga tcatcggttc atcaccctgc
     9721 acattaacga tcaccgtgtc gtcgctgaat gcgcattttt cgacaacttc cgccagacgt
     9781 tctgttcctg actgatgatc ggcgcgcgtc atacatactt caccgccagc ggcttcaacg
     9841 gcgcgggcaa catcatcatg atcggttgcc acgatgatgc gctcggcacc tgattcacgc
     9901 gcgcgttcaa gaacatgaac aatcatgggt ttgccgttaa tatcaaccaa tggtttaccg
     9961 ggcagacgcg tggacgcgta gcgcgcggga ataatgacca caaaactcat gatttactct
    10021 catcagcagt cagcacgcgg gcttccgttt ccagcaacac tggaatgcca tcgcggagcg
    10081 ggaaagccag gttatccagt ttgcaaataa gctcttgttt ttcctggtta taccaaagtt
    10141 ttccgttgca aaccgggcag gcaatgattt caagcaaacg atgatccata gttcctccag
    10201 atggatcggg ttatgaatgc ataaatctta tcataatcat ttatgcgacg gggtctattt
    10261 cccagccagt gcggcaatca gtaaagagac cttgcgggca gcgtcctaat gtcacccgcg
    10321 ttgcctgctg ccagctcgcg aaatcagtaa tcgcctgacg taaccctttt tgcagcattg
    10381 tcgttggttt aatgccttcc tgtaaccaca gagagataac ttcaaggatg cctgtctggc
    10441 gatgcatttt ggcatccatt cgcccaacta attgcccacg atgtaataat ggcagaacaa
    10501 aatagccata ctggcgtttc ggcgctgggg tatagcactc cagccggtag ctaaaatcaa
    10561 aaagctgctc tgcgcgtttg cgatcccaga caacaggatc aaaaggcgaa agtaccgcgc
    10621 tgtgcgttgc agtgagcttt ccggccagcg cacgctcgag tagcggcagc aaatcagcat
    10681 gcagccaaag attgcccaat ttttcaacat gcacagcaat gatttgctgc tgttcagccc
    10741 tcgcttcgcg ccacgccgcc agtgccggac gtttcagccg atagtaatct gccagccact
    10801 gttcgcggaa tattcccagg ctacgcgcac tgttatccag catgatgatt tctgcttctg
    10861 tttgcgaaac gagatcgcgc tcatcatccc agtcaggcat gacacggtgg gttaaatcat
    10921 aaacgcgctg gaagttgcgc cgttcaatca ccatcacctt tccggcagta aataaacctt
    10981 ccagatgccg tttatgcggc ttccattccc accagccgct tgcaccttta cgaggatgct
    11041 caaaatcggc tgaacgtacc ggccctttat catgaatatg ctgaattaac tgtgcaattt
    11101 ccgcctcatg ttcctgcatc caggcgtctt tgtatttcca gcccattttt tcaggtgcca
    11161 gcatgcggtg gcgaataaga cgaaagtcgc tacgcggcat aaagcaggct tcatgcgccc
    11221 agtattccat taattcgcca cgcgccagag actcatccag ccactgggca ggataatttc
    11281 ccagacgact gaaaagcacc agatatggac tacgggcaac aatattgatg gtatcgattt
    11341 gcagcaagga catgcgggag atcgttgccg gaatatcctc caacgacgct cgacggcggg
    11401 gtttgtttaa caggccttgt gcggcaaggt gaagattacg cgcatcagca agggagaggt
    11461 gcggcagcga cattcatgac tccatcaatc gaacgctgcc gcggcgtaac tagttgccag
    11521 aagccagcga ggttagttgc gtaagcagtt tcgctggttc atcacctgaa agctgtgcgt
    11581 ctacaggcaa ataccaccaa ttttcttctg caaaggcccg gcatttcacc gcatcttttt
    11641 cagtcattac cagcgtttgc ccggcgctta ccaacgcact gacatccgca tggttcaaag
    11701 actgatgatc ggccagcggt acacattttt ccggttgtac gccacacatc ttcagcgtgg
    11761 caaaaaagcg cggcggatgc ccaatccccg ccatcgccac tacatgttca agctgagcaa
    11821 cgtcacaacg cgtaccggta cgtaaattca ccgcctgacc cggcagcaga tgcatgggga
    11881 tttcaccgct gcgagggaca ccgccgttga cgattaccgc atcaaccgac tttaagcgcc
    11941 ccgctcgctc acgcattggc cccgccggca accaccagcc attgccaaag cgacgcacac
    12001 catcaataac gacaatttcc acatcacgcg ccagacggta atgctgtaaa ccgtcgtcgg
    12061 ttacgatgat ctgcacatca gggtgttgcg ccagaatggc ttttaccgca tcagaacgaa
    12121 cgggagaaac cgcaacaggc gcatcagtgc gttgataaat caacacaggt tcatcacccg
    12181 cctgtgctgt ggtggtatct gccgacaata acagcggata agattcagcc ttaccaccat
    12241 atccccgcga tacgaccccc acgcgaatac cgcgctgttg caactgttcc accagccaga
    12301 caacgaccgg ggtttttccg ttgccgcctg cggtgagatt accaaccacg acaaccggta
    12361 cgggggcacg ccaggcgcgc ttcagtttta gtttatagca aagacggatc gcgccactca
    12421 ccaggccata caaccaggag agtggcagca atagccgcca caaaggggat tcaccagacc
    12481 agattttttc gatcattggc caaactgcat tttgtgaagt tgcgcgtaaa cgccgcggtg
    12541 ctcaagcaaa tcgttatgcg taccgcgttc cacaatgaca ccatcctcga cgaccacgat
    12601 ttcgtctgcc ttttcaatgg tagacaagcg gtgggcaatc accagagagg tacggttttt
    12661 ctgcaactca tccagtgccg cctgaatcgc acgttcggat tcggtatcca gagccgaggt
    12721 agcttcatcc agaatcagaa tcgggctatc acgcagcaag gctcgagcga tagcaatacg
    12781 ctgacgctga ccgccagaga gcagcacgcc gttttcacca atcactgtat cgagaccgtt
    12841 atccatctta ttgatgaagt ccatggcgta ggccatccgc gccgcttctt caatttgctc
    12901 acggctgtac tgttcagtcc gtgcgtaagc aatgttgtta gcaaccgtat cgttaaacag
    12961 atggacattc tgcgacacca gagcaacctg gttacgtaac gacgccaggg tatactcgcg
    13021 cagatcgtga ccatccatca ggatttcgcc ttcatcaata tcgtaaaaac gcgtgatcag
    13081 gctggcgatg gttgatttac ctgaaccaga gcgtccaacc agagcaaccg tcttccctgc
    13141 cggaattttc aggttgatgt tacgcaatgc gggtacgtca cgtcccggat aagtaaaggt
    13201 gacattgcgg aattccacgt cgccagtcgc acgctcgatc acgcgcttac cttcgtcttt
    13261 ctcctgctca ctgtccagaa tggtaaacag cgtctgacaa gccgccatac cgcgctggaa
    13321 ctgggcgtta acgttggtca gcgatttcag cggacgcatc agtgcaatca ttgaagagaa
    13381 aacaacggta atcgtaccgg cagtcaggtt atccataaca cttgggaagc tcgccgcata
    13441 cagaacaaac gccagcgcca aagaggcgat cagctgaatg atcggatcag agatggaaga
    13501 ggctgaaacc attttcatcc cctgaagacg cattcggtta ctgactttat cgaagcgttt
    13561 cgtttccact tcctgaccac cgaaaatcaa tacttctttg tggcccttca gcatttgttc
    13621 tgcactggtg gtcacttgcc ccatggtgtt ctgcatgttt ttactgatgt tgcgaaaacg
    13681 cttcgatact acgcgaatcg caatcgaaac aatcggtgcc agcacaatca aaatgatcga
    13741 cagttgccaa ctgtaataga acatcatgat gaacaggccg atgatcgacg caccttcacg
    13801 cacaacagta atcagtgcgc cggaagaaga agaagcaacc tgttcggaat cgtaggtgat
    13861 acgtgacaac aacgtacccg ttgactgttt gtcaaagaat gaaactggca ttcccatcat
    13921 gtgaccaaac aggcggcgac gcatggtcat taccaccttt cctgataccc aggagataca
    13981 gtagctggag acatagctgg tgataccacg taaaatcatc agtccgatca ccaccagcgg
    14041 catccacacc agcacggagc gatctgtttt accaaagcca tcatcaagaa gtggcttaag
    14101 gagcgataac atgaaggtat cgctggctgc gttgaggatt aacgctacgc ccgccacgat
    14161 cagacccgct ttgaaaggcg caatggttgg ccacagtcgg cggaatgtct gccacgtaga
    14221 gagatctttg tcgttatgca ttcaaaaaac cagcatttgt tgaaatagcc gcatattcta
    14281 cccgttatcc actggcacgc caaaccactg atgataccaa cgaggtaaaa tttgctctct
    14341 gaggctgcta atccgccagc cttgtgctga aaaattgacc gtcacttgac cctgatgtgg
    14401 agtatcaagc cattgatatc ctttctgttg atagcgatgc ttaactttgt tagagggcaa
    14461 tcgccatgcg ttatagcgcg atgccgatgc gagtgccact tttccattca ctcgctgaat
    14521 taatggcaac gatgatgagg tattactgcc atggtgaggt acctgaagca atgttgcctg
    14581 cacttgctgc cagtaacggc ttagcatctt ttgttcagct ggggcttcaa tatcaccggt
    14641 tagaagaatg ctattcgtcc cgtcatcaac cttaaccaca caggaatggt tatttccctt
    14701 atcgttgcta ccttgtaaag gccagtgcgc gctgaaacgc aatccttgcc actgccacgc
    14761 ttcgccacgc acacagggct gatgatgttc ccagtttaac ggacttctga tccataacat
    14821 cggccatata tgcaatattg aatccagccc tccccggtga tccagatgtt catggctcag
    14881 aataacgcct tccggttcaa gattatgcca gtggagccag gggataatca gttgttgccc
    14941 actatcccct tcaggccagt cctgtgtcat agagaatcgc tttgccgttt ctggctatca
    15001 ccattgccag cccttgcccg acatcaagca tgtacagctg ccactcgtca ggtcgaggtt
    15061 tttgccacag cggccagcac atcagcaagc ctccagccac acacattgct ggcaacgttc
    15121 gccaggcgtt taatcgccat accaccagta agaaccatgg ggaaaatgat agccattgcc
    15181 aacattcaac aatgttgatc cacccttccg gcaaactctt taacccccag aaaagtaaag
    15241 ccaaagaccg gtcggcaaga aaccataacc cctgttctag gattaacggc ccgctaaaat
    15301 gcacaaccat cgcggcgagg atcaacggaa ccgtgataaa tgtcaccaag ggaattgcaa
    15361 atagatttgc aataaacgag gtcagactaa tgccatgaaa tatgacgatt tgcatgggca
    15421 taagcaggag tgtgattccc agttgcagat ggatgaggga aacaactgca cgcaataccg
    15481 gcggcagttg ccactcagga cagggaaacc actgatacca aaaaatcagt gccgcgaccg
    15541 cagcggcaga gagccataaa ctttgcgaga gaatggcaac aggatccatc agcaaaattg
    15601 ccgccagaca acatatccat acatcccagc cactccactg tcgcccactt aacttaagca
    15661 ttccccacgt agcaagcgcc accacggtac gcaatgcagg aggttgcatt cccgtcaacc
    15721 aggcataaaa agcagcacag cagattccgc caattaatgg tatttgccag tggatccagc
    15781 gcccaggcag aaaaatttgt ccactgcgaa tgagtccggc agccagcaac gccgcaaaag
    15841 cgatgtgcaa tcccgatatc gccattaaat gcgccgttcc agtatcgcgc atgatatttt
    15901 tgatttcttt ggggacggat aaccgttccc ccatacctaa accaagaata accgcattcc
    15961 acggataggg ttgcagcgtt gtttgtagtg acgccagata ctgtgcacgc aggctacaat
    16021 tcggttcaat gacacttgcc tgcagaaaac ggccggtgag cggctgatgc tgggcaatgg
    16081 cataacgctg gctatcaaag ccgccatcat taagttggcc atgaactgca cgaactttga
    16141 gtttcattga ccattgttgt ccagcacaaa ccgcttgcgg aagttattcg ccatacagca
    16201 caaggcctgg cgcagggaat atacgtcgac cttgtagata agtaatttga ccgtaatgag
    16261 tggtcatgcc gtcagttgca gtgatctcaa caattgcatc ctgcgtcgcg ccagttaagg
    16321 tttctcctgc ccacaaaatt tgctttgctg ataatatgcc ccaaacgaaa aacagcaacg
    16381 tcagcgcgat ataacggacg gttttaacag ggataaatgc cagtacgcag gcgaagagag
    16441 tcagaaacgc aagggttaat gtcctaggca attggggcaa aatcagcaac ggaaaaattc
    16501 cgcaaattat gcatacgccg actgtcgtta ttttcatgca cccctccctg tttatggaaa
    16561 gtgtgcaact ttgttgagcg gttgtctggt gggaattttg cgttccccga ggcacattca
    16621 gaagaattta acgaaagggt gaaaactgct caaaactctg cgaggcagct tccagttcct
    16681 gattagagaa ttgaaaaaag cacccgacag gtgcttttct cgcgttcaag tttgagtaaa
    16741 aacttaaccg taaatattgg cgcgatcgcg cagttcttta ccaggtttaa agtgaggaac
    16801 gtattttcct tccagttcta ctttatcgcc agtcttcgga ttacgtccgg tacgtggtgc
    16861 gcggtagtgc aaagagaaac tgccgaaacc gcggatttca atacgctcgc cctgcgcaag
    16921 agtcgaggcc atatgctcca gcatctcttt tactgcatct tcaaccgtct tggcgggaat
    16981 gtgcgattgc tgggtggcaa gtctttctat caattctgac ttggtcatga ttcctccggt
    17041 tccttaaagg caaattaagc ggctgctgtt gctgcattga ttaagggcgg ccgtagccgc
    17101 ccttagtgct tgattacagg acgaaacctg caatctgtca agtaaactca acaaacttcg
    17161 gaataaaaat cccgaagagt cagagaatta ctcgccttta gctgctttga aagcttcagc
    17221 cattgcgttg ttggagaagt ttgcatcttc ctgtttgtta acagttgcga ttgcatcttt
    17281 ctcgtcagct tcgtctttcg cacgaacaga caggctgatt gcgcggtttt tacgatcaac
    17341 gccggtgaat ttagcttcaa cttcgtcgcc aacgctcaga accagggtag cgtcttcaac
    17401 gcggtcacgg gatgcttcag aagcacgcag gtaaccttca acgccgtcag ccagttctac
    17461 ggttgcgcct ttagcgtcaa ctgcagttac tttaccggtt acgatagcgc ctttcttgtt
    17521 cagagcaacc cagttgttga acggatcttc tgcgagctgt ttaacgccca gggagatacg
    17581 ttcacgttct gcgtcaacct gcagaacaac tgcagcgatt tcgtcgcctt ttttgtattc
    17641 acgaactgct tcttcgcctg caacgttcca ggagatgtca gacaggtgaa ccaggccgtc
    17701 gatgccgccg tccaggccga tgaagatacc gaagtcagtg atagacttga ttttaccttc
    17761 aacacggtcg cccttgttgt gggtttccgc gaactgctgc cacgggttag ctttgcactg
    17821 tttcagaccc agggagatac gacgacgttc ttcgtcgata tccagaacca taacttccac
    17881 tacatcgcca acgttaacaa ctttggacgg gtggatgttt ttgttggtcc aatccatttc
    17941 ggaaacgtgt accagacctt caacgccttc ttcgatttca acgaagcagc cgtagtcagt
    18001 caggttggtc acgcgaccag tcagtttggt accttccgga taacgtttag cgatagctac
    18061 ccacggatct tcgcccagct gtttcaggcc cagggataca cgggtacgtt cgcggtcgaa
    18121 cttcagcact ttaacagtga tttcgtcgcc cacgttgacg atttcgctcg gatgcttaac
    18181 gcgtttccag gccatgtcag tgatgtgcag caggccgtca acgccgccca gatcaacgaa
    18241 tgcaccgtag tcagtgaggt tcttaacgat acctttaact tccatgcctt cctgcaggtt
    18301 ttccagcagc tgatcgcgct ctgcgctgtt ttcggattcg ataacggcac gacgagaaac
    18361 aacaacgttg ttgcgcttct gatccagctt gattacttta aattcaagct ctttgccttc
    18421 caggtgcaga gtgtcacgca ccggacgaac gtctaccaga gaacctggca ggaacgcacg
    18481 aataccgttc agctcaacag tgaagccgcc cttaactttg ccgttgataa caccggtaac
    18541 agtttcagca tcttcgtaag ctttttccag cgtgatccag gcttcgtgac gtttagcttt
    18601 ctcacgggac agcagagttt caccgaagcc gtcttctact gcgtccagag caacgtcaac
    18661 ttcgtcacct acctggattt ccagctcgcc ctgggcgttt ttgaactgct cagccgggat
    18721 agcggactca gatttcagac cagcgtcaac cagtactacg tctttgtcga tagcaacaac
    18781 aacgccacga acgatagaac ccgggcgggt ttcgatttct tttaaggact cttcaaagag
    18841 ttgagcaaaa gattcagtca tgtttaatct tcaggtttat atttaacgtc cacctagctc
    18901 catgccggat ggggttgttt aacatacccg ctgtcattcc attgcaacgg gggtactgca
    18961 aattcggtcg cttatgcgag agccaatttc tggcgcgcgt attgtagcgc tttttcaatc
    19021 aattgctcaa tgcttaaggt ggtggaatcc aacactaaag catcggctgc cggaaccagt
    19081 ggcgctaccg ctcggttacg atcgcggtcg tcgcgttctt tgatctcggc caaaaggcgc
    19141 tcgaagttaa cactaaagcc cttctcctgc aactgtagca tgcggcgatg cgcacgttct
    19201 tccgaggagg cgtcaaggaa aattttcact ggtgcatcag ggaataccac cgttcccatg
    19261 tcgcggccat cggcaatcag acctggtaat tcgcgaaacg cgcgttggcg acgcaataat
    19321 gcttcacgaa cgcgtgggaa tgccgcgact tgtgaagccg cattcgccac ttcctgagta
    19381 cgaatttcgc cgctgacatc ttccccttcg aggatcactt ccagattgcc attggtcgac
    19441 acaaaacgta catccagatg ggatgccagc ggtaccagcg catcttccga cgcaacatca
    19501 acatggtgat gtaatgccgc caatgccagt acgcgataaa ttgcacccga gtccagcaga
    19561 tgccattgca acgcttccgc catagcctta cacaaggtgc ctttccctgc accgcttggg
    19621 ccatcaatgg taataaccgg ggcaattgcc gtcatcttta tctccttaaa acaggcgtac
    19681 cgttaacata accgcgcgca ttatacgcgc cgacgccaca aatcgttaac gttgtgtgca
    19741 aaaaccagtt ttgtctgtat tcagatgggg atttattgcg caattatagc gggctggcag
    19801 aagaccagcc cgaaagatga caatgattta cttagccaga ggcgatacga tcacggatgt
    19861 gttgcgcacg ctctgttgat ggtgggtgag agtcaaacat ggactgggtg cgaccgccat
    19921 ccaggctagc cagtttttca aagctgccaa ccagcccctg ggtgctgatg ccacgtttct
    19981 tcaacagatc aaaagagaaa tcatctgctt cggactcttt atcacgggag tatttagcat
    20041 taatagcgcc ttctgcgata tcacccaatt gtgagcggga aagctgggaa gccacaccgc
    20101 tggtagctga aatggcatcg cgtgcggcaa cgatcgcata agaagctttc atttcagcca
    20161 gcgagtgacc caacgcgacg tggcccagtt catggcccag aacaccttca atttcgttgt
    20221 cattcatcat gtccatcagg ccactgtaga cacgaacaca gccgttcgcc atcgcccatg
    20281 cgttgacgtc gctggtcata taaaccttat agttgaccgg cgtgccatta atattgttac
    20341 ccaatgcttt ggcgattttt gccagacgtt taccgtactt gctggattta cttgcgactt
    20401 gattgccgct gtccatttcg gcacagccct gattggcaat cgcttttgca tcggcatcgc
    20461 ttaatgttgc tgctttatag gcatttaaac cggagctgat agccatattg gtatcaatac
    20521 cgtgggtatt ttgacaccct gtcagtaatg ctgcagaggt cgcaatcgcc agcagtaatt
    20581 tagtattctt catgtgaatc atccgttgat taatcatata taaatattat gtatcaacaa
    20641 cttacattga caagcgacgc taccattaat caaaatgact cacaaggtcc gaaaaaaaac
    20701 gccgacaatg tgc
//
LOCUS       DAJTQY010000068        20691 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.068, whole genome shotgun sequence.
ACCESSION   DAJTQY010000068 DAJTQY010000000
VERSION     DAJTQY010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20691
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.068"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            82..351
                     /locus_tag="OO349_002630"
     CDS             82..351
                     /locus_tag="OO349_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HCR5867652.1"
                     /translation="MQTVKLRQQGGAMIVTIPRDLAIDLGWSPGTELTVEKKGDSVNL
                     RATEHKPRGRLTVAQLLSQIDESEITELNQSTEGWAEGKKGNEAW"
     gene            345..710
                     /locus_tag="OO349_002631"
     CDS             345..710
                     /locus_tag="OO349_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCR5867653.1"
                     /translation="MVKARTPHRGEIWYFNPDPVAGHELQGPHYCIVVTDKKLNNVLK
                     VAMCCPISTGANAARSTGVTVNVLPRDTQTGNLHGVVLCHQLKAVDLIARGAKFHTVA
                     DEKLISEVISKLVNLIDPQ"
     gene            complement(711..1499)
                     /gene="bioH"
                     /locus_tag="OO349_002632"
     CDS             complement(711..1499)
                     /gene="bioH"
                     /locus_tag="OO349_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HCR5867654.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRVLVVSES"
     gene            1537..2220
                     /gene="gntX"
                     /locus_tag="OO349_002633"
     CDS             1537..2220
                     /gene="gntX"
                     /locus_tag="OO349_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HCR5867655.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            2279..2854
                     /gene="nfuA"
                     /locus_tag="OO349_002634"
     CDS             2279..2854
                     /gene="nfuA"
                     /locus_tag="OO349_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HCR5867656.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            3215..4531
                     /gene="gntT"
                     /locus_tag="OO349_002635"
     CDS             3215..4531
                     /gene="gntT"
                     /locus_tag="OO349_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCR5867657.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(4576..6660)
                     /gene="malQ"
                     /locus_tag="OO349_002636"
     CDS             complement(4576..6660)
                     /gene="malQ"
                     /locus_tag="OO349_002636"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HCR5867658.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWQLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYKNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(6670..9063)
                     /gene="malP"
                     /locus_tag="OO349_002637"
     CDS             complement(6670..9063)
                     /gene="malP"
                     /locus_tag="OO349_002637"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HCR5867659.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVMKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLTDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VCLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENLQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLNAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            9675..12380
                     /gene="malT"
                     /locus_tag="OO349_002638"
                     /pseudo
     CDS             9675..12380
                     /gene="malT"
                     /locus_tag="OO349_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
     gene            complement(12423..13439)
                     /gene="rtcA"
                     /locus_tag="OO349_002639"
     CDS             complement(12423..13439)
                     /gene="rtcA"
                     /locus_tag="OO349_002639"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HCR5867660.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITGIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGDYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFSTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGIFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(13443..14669)
                     /gene="rtcB"
                     /locus_tag="OO349_002640"
     CDS             complement(13443..14669)
                     /gene="rtcB"
                     /locus_tag="OO349_002640"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HCR5867661.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPFNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESEQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQLFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            14858..16456
                     /gene="rtcR"
                     /locus_tag="OO349_002641"
     CDS             14858..16456
                     /gene="rtcR"
                     /locus_tag="OO349_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HCR5867662.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCIYQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVGVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSTPRKKEQPRGPGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(16438..17196)
                     /locus_tag="OO349_002642"
     CDS             complement(16438..17196)
                     /locus_tag="OO349_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HCR5867663.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(17213..18043)
                     /gene="glpG"
                     /locus_tag="OO349_002643"
     CDS             complement(17213..18043)
                     /gene="glpG"
                     /locus_tag="OO349_002643"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HCR5867664.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWLAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYMQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(18088..18414)
                     /gene="glpE"
                     /locus_tag="OO349_002644"
     CDS             complement(18088..18414)
                     /gene="glpE"
                     /locus_tag="OO349_002644"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HCR5867665.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            18604..20109
                     /gene="glpD"
                     /locus_tag="OO349_002645"
     CDS             18604..20109
                     /gene="glpD"
                     /locus_tag="OO349_002645"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HCR5867666.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYTRTYGSNSELLLGNAGTVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(20378..20608)
                     /locus_tag="OO349_002646"
                     /pseudo
     CDS             complement(20378..20608)
                     /locus_tag="OO349_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443257.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
BASE COUNT         4889 a         5689 c         5217 g         4896 t
ORIGIN      
        1 agtcagtaga tactctaccc tgcttaagtg ctactttgtg ctatcttgca tgtaacacaa
       61 agtagcactt agggaactga tatgcagaca gtgaaattac ggcaacaagg aggagccatg
      121 atcgttacta tcccacgaga tcttgccatc gatcttggct ggtcaccggg cacagagctt
      181 accgtggaga aaaaaggcga tagcgttaat ttgcgcgcca cagagcacaa accgcgtggg
      241 cgtcttaccg tcgcgcagtt gttgagccaa atcgatgaaa gcgaaattac ggagctaaat
      301 cagtccacag aaggatgggc tgaagggaaa aaaggtaatg aagcatggta aaggcacgga
      361 cgccacatcg tggtgagatc tggtatttta accctgatcc ggttgccggg catgaacttc
      421 aggggccaca ttattgcatt gtggtaacgg acaaaaaact caacaatgtt ttaaaagttg
      481 ctatgtgctg cccgatttca acaggggcaa atgcagcacg ttccacaggg gtgacggtga
      541 acgtcctccc ccgtgatacg caaaccggta acctgcatgg cgttgtactt tgtcaccagc
      601 taaaagccgt cgatcttatt gcccgtggcg ctaaatttca taccgttgcc gatgaaaaat
      661 tgattagtga agttatcagt aaactggtga atttaatcga cccacaataa ttagctctca
      721 cttaccacca acaccctctg cttcaacgcc accagcaggt gacaaaactc gaccggatgc
      781 gaaataaacg gcgcatgggc cgctttggcg aagatatatg attcgctgtg aggccaaagt
      841 ttatccagca tcggcaccac tttgcgcggc accagaccgt cgagatagcc atacaatcgc
      901 aaaaacggca tggacacgtt ttgcagcggc tgacggagat cgaccgtttt caggatttcc
      961 agcccgccat taagcacgtc aacctccggc atcggtaacg ccagaacggt tttcttcaac
     1021 gcccgcgcat cctggcgcgc cgtttcagtc cccatggttt gtaacgccag gaaccgctcc
     1081 actgtacgct gaaaatcatc actgagttgc tgctgaaatc ccgccagcac gtccggtttt
     1141 atccccggcc actcgtcacg agcactaaaa caaggtgacg acgccacggt gaccagcgcc
     1201 tgaacacgct cgggatgggt taacgcaatc tggcttgcca ccagcccgcc cagactccag
     1261 cctaaccaaa tggctttatc aggtgcctgt tgcagcacgg cttcggccat atcagcaagt
     1321 gacagcgcac caaatccccg gctacgcccg aagccgggca ggtcaacaag gtgcagcgta
     1381 aaatgcgagc taagttcctc gtcaatgcaa cgccacactt cggcattcag tccccatccg
     1441 tgcagcagca caagatgaac attcccctga cctttggtct gccaccagat gttattcatc
     1501 cgctattgtt ctcttttgac ttacaaggat gaacatatgc taacagtacc gggattatgc
     1561 tggctatgcc gaatgccact ggcgttaggt cattggggga tttgttcggt ctgttcacgc
     1621 gccacccgca cagataaaac gttatgccca caatgtggat tacccgccac acactcccat
     1681 cttccctgcg gtcgctgcct gcaaaaaccg ccgccctggc aaagactggt cacggttgcc
     1741 gactatgcgc cgccgttaag tccgcttatc caccagctta aattttcccg gcgcagtgaa
     1801 atcgccagcg ccctgtcacg tctgttacta ttggaagtct tacacgctcg tcgcaccacc
     1861 gggttgcaat tgccggatcg catcatcagc gttccgttat ggcagcggcg tcactggcgt
     1921 cggggattta atcagagcga tttgctgtgt cagccgttat cacgctggtt gcactgccaa
     1981 tgggatagcg aagccgtcac acgtacacgg gccactgcga cccagcattt tctcagtgcc
     2041 cggctgcgca agcgcaacct gaaaaatgcc tttcgtcttg aattgcccgt gcaaggtcgc
     2101 catatggtga ttgtggatga tgtcgttacc accggaagta ccgtcgcaga gattgcgcag
     2161 ttgcttttac gcaatggtgc ggcgactgtc caggtctggt gcctttgtcg aaccttgtag
     2221 agcctcgatg atgggcgtat tataaccaac taaaatagtc aactattagg ccattactat
     2281 gatccgtatt tccgatgctg cacaagcgca ctttgccaaa ctgctggcaa atcaggaaga
     2341 agggacacaa atccgcgtat ttgtgattaa ccctggcacg cctaacgctg aatgtggtgt
     2401 ttcttattgt ccgccggatg ccgtggaagc caccgacact gccctgaaat ttgacctgct
     2461 gaccgcgtat gttgatgagt taagcgcacc atacctggaa gatgcagaga tcgactttgt
     2521 taccgaccag ttgggttctc agttaacgct gaaagccccg aacgccaaaa tgcgtaaagt
     2581 ggcagacgat gcaccgctga tggagcgcgt ggagtatatg ctgcagtcgc agatcaaccc
     2641 acagcttgct ggtcacggtg gtcgcgtttc gctgatggaa atcaccgaag acggttacgc
     2701 cattctgcaa tttggcggcg gctgtaacgg ttgttccatg gtcgatgtga cgctgaaaga
     2761 agggatcgag aagcagctgc tgaacgaatt cccggagctg aaaggtgtgc gcgatctcac
     2821 cgaacaccag cgcggcgaac actcctacta ctaagttatc ccctcttttg tagattgccc
     2881 gatgcgacgc taaagcgtca tttcgggcct acgttgaaca tgtgccagac gttggcacag
     2941 cattgctttg gcaaatccct cccttcgttt ccccgcgata atatgaccaa cctctcataa
     3001 tttaaattta ccccgctctg gtgattctca aacgccaaat gttacccgta tcattcacat
     3061 gggtaccaaa catactcctg acatctgact acaataatta gttttagtgg gtatcagtcg
     3121 tggtgccgca atatctctgt tcccgattgg gataattaga gtttgtcgtc agaaaattga
     3181 cgttacccat aacaaatgaa aggccaggta aatcatgcca ttagtcattg ttgctatcgg
     3241 tgtaatcttg ttgttgctcc tgatgatccg cttcaaaatg aacggcttca tcgctctcgt
     3301 cctcgtggcg cttgctgttg gattaatgca aggaatgccg ctggataaag ttattggctc
     3361 catcaaagcc ggtgtcggcg ggacgctcgg tagccttgcc ctgatcatgg gttttggcgc
     3421 aatgctgggc aaaatgctgg cagactgcgg tggcgcacaa cgtatcgcca ccacgctgat
     3481 tgccaaattt ggtaaaaagc acatccagtg ggcggtggtg ttaaccggtt ttaccgttgg
     3541 ttttgccctg ttctatgaag tgggcttcgt gctgatgctg ccgctggtgt ttaccatcgc
     3601 ggcttccgcg aatattccat tgctgtatgt tggtgtacca atggcggctg cactgtctgt
     3661 gacccacggc ttcctgccac cgcatccggg cccgactgcg attgccacca ttttcaatgc
     3721 cgatatgggt aaaaccctgc tgtacggtac tattctggca atcccgaccg tgattctcgc
     3781 cggtccggtt tacgctcgcg tgttgaaagg tatcgataag ccaatcccgg aaggtctcta
     3841 cagcgcgaaa accttcagcg aagaagagat gccgagcttt ggcgtcagcg tctggacctc
     3901 tctggtgccg gttgtgctga tggcgatgcg tgcgattgcc gaaatgatcc tgccgaaagg
     3961 tcacgctttc ctgccggtag cggagttcct cggtgacccg gtaatggcaa cgctgattgc
     4021 cgtgctgatt gcgatgttca cctttggtct gaaccgtggt cgttcaatgg atcagattaa
     4081 cgacacgctg gtttcttcca tcaaaatcat tgcgatgatg ctgttgatca tcggtggtgg
     4141 cggtgcgttc aagcaggtgc tggtagacag cggcgtggac aaatacattg cttccatgat
     4201 gcacgaaacc aacatttctc cgctgctgat ggcctggtcg attgctgccg tactgcgtat
     4261 cgcgctgggt tccgcaaccg ttgcggcaat cactgcgggt ggtatcgcgg caccgctgat
     4321 tgcaacgacg ggtgttagcc cagagctgat ggttattgcg gtgggttccg gtagtgtgat
     4381 tttctctcat gtgaacgatc cgggcttctg gctgttcaaa gagtacttta acctgactat
     4441 cggcgagacc atcaaatcct ggtcgatgct ggaaaccatt atctcggtgt gcggtctggt
     4501 aggctgtctg ctgctgaata tggtgatttg ataacactga ctgccggatg catcgcatcc
     4561 gacatcaacc gcactctact tcttcttcgc tgcagctctg cgccgtctgt ccaaatcctt
     4621 cagcaacttg ttcacgccat catcggcaaa catcgactca agcgttgcgg aaagcttgcg
     4681 tcgccagttt ttatactggt aactggtgcc aggaatattc accggttcgg ccatatccag
     4741 ccagtcttcc ggctgtagtc ctaacagagc actgttactg tcggcaatgt agcgctgcaa
     4801 accacggttc agcgtcggcg tcatcgacat caacgatgcc ttatgcccgg cacgttttgg
     4861 cagacaacca tatttatgca gtgcatccag cagcccttgc ttcgccagtt cgcgatcctg
     4921 atacagaccg cgcagtacca cttcatccgg atacagcccc agggttttgc ccagcgttag
     4981 atccccgctc tcccagtaac cacgcagcgt tggcaggtca tgtgtcgccg caaccgccat
     5041 cgactgctcc ggatacgctt tcggtgcacg gaacgtcttc tcgtggtcgt tttcgaaata
     5101 gagcactttg taagagtaca caccgctgct gcgcagctta ccaacaatct ctaccggtac
     5161 ggtaccgaga tcttcaccaa tcaccataca gcgatgacgt ttactttcga gtgccagaat
     5221 cgagagcaga tcatccaccg gatagtgaac atacgcgccc tgatctgccg tctcgccata
     5281 cggtatccac cacaaacgca gcatcgacat cacatggtca attcgtaatg cgccgcagtt
     5341 ttgcatattg gcacgcaaca gctcgataaa cggttcatag gcacgcgcgg tgatgatatg
     5401 cgggtccatt ggcggtaatc cccagttctg ccccaacggg ccgaggatat ccggcggcgc
     5461 gccaaccgat gctttcaggc aatatagttc acggtcacac caggtttccg ccccaccttc
     5521 cgctacgcca accgccagat cacgatacaa gccaatcggc atttcatagc cctggcttat
     5581 ctcccagcag gcggcaaact ggctgtaagc cagccactgc aaccagagat aaaaatcgac
     5641 gtcatcacga tgttcttcgc agaactgacg cacttctggt gaatccacgt tcttatacat
     5701 ctctggccat gcaggccagc cccagcgcat ttcgtcctct ttcacttgct gggcatgtag
     5761 cgcatcaaag gcagcctgcc agaacaggct gtcgccctgc tctgcaacaa actggcgaaa
     5821 cgcggccatc tgctcatcat cacgttgcgc gaaacctttc cacgccattc gtaatgctgt
     5881 catttttagg gcggtaaccg tggagtaatc gacccagtcg gcatcgcgcg cctgttgcag
     5941 cgtctgttgc gtggtcggca actgccacca ggcctgagcc tcttcgctaa gatggaaatc
     6001 ttcaacggcg ttaacgtcga tataaatcac attcagccaa cggcgagaag acgggctgta
     6061 tgggctggcg ctctccggat ttgccggata gagcgcatga atcgggttca ggccaatgaa
     6121 cgacccgcca cgttttgcca catccaccag catcgctttg agatcgccaa aatccccaat
     6181 accccagttt ttttccgatc gcagcgtata aagctgaacg caggcacccc acagcttttg
     6241 tttattcagc aaagcctgcg gttcgtaaca acgtttcggg gcgacaatca cccggcaatg
     6301 cgcgcgctgg tcgtcctggg tgagtgttag cgtgtgataa ccttccggca gcttcgtcgg
     6361 tagattgaac gctttgcccc ccgttacatg gcctttgtac tgcgttcctt cttcggtggt
     6421 cagctgccag ctatattcgc cgctgccctc caccaccatc ggcatttttt tgccgctggt
     6481 ataaaccatg acattcggga ctggcgttac cgccactttc gtggcggtac gttgatgcat
     6541 cgcgtcaagc aaacgccgtt tggtttcggc gctaatcgac tgcggtttac cgtgggcatt
     6601 gatgtaattg gggctaatcc ccgccgccag cgcggcatta tccagacgtt tgctttccat
     6661 cgagcttcct tagcgttttg cctgccagat acgagcctga taatcgcgaa tagagcgatc
     6721 cgagctaaac ataccgcagc gggcggtatt gaggatcgcc gcgcgagtcc aggcctcctg
     6781 gtcgcggtac agcacatcca cctgcttttg tgcctcaaca taggctgcga aatccgccat
     6841 caccagatac ggatcgccgc cctgtttgcc gatactgtgc agcatctggt cgaaggcatg
     6901 cttatcgccg tcgctgtatt taccgctttc cagctctttc aatactgcgt tcagcacctt
     6961 atctttcttc cgccatttca ccgggtcgta gcctttggcc agaatagcct tcacttgttc
     7021 cacggtatga ccaaaaataa agatattttc ttcaccgact ttctcggcga tttcaacgtt
     7081 cgcaccatcc agcgtaccga cagtaagcgc accattgagc gccagtttca tattgccggt
     7141 accggaagct tctttacctg cagtcgaaat ttgttcggag atatccgccg ccgggatcag
     7201 tttttccgcc gccgaaacgc aataatccgg caggaacacc accttcaact tatcgccaac
     7261 cagtggatcg ttgttgatca cgtcagccac tttgttgatc gcaaagataa tattcttcgc
     7321 caggtagtag cccggtgccg ctttcgcgcc gaagaggaag acgcgcggta cgcgatcagc
     7381 ctgcaggttt tcacgaattt ctttgtacaa cgccagaata tgcagcagat tcaggtgctg
     7441 gcgtttgtac tcatgcagac gtttgatctg aatatcgaaa atcgcctgtg gattgatctc
     7501 aataccggta cgaactttca caaactccgc cagacagact ttattcgcct gcttgatctc
     7561 gcgatattgc tgacggaatt tcgcatcatc agcgaatttt tccagattga tcagctgatc
     7621 gagatcgtta gcccactctt tttgcagtga tttatccaac agagccgcca gtgcagggtt
     7681 gcactgtttg atccagcgac gtggggtaat accgttggta acgttatgga atttgttcgg
     7741 ccatagctgg tgatattccg ggaacagatc tttcaccacc agatccgagt gcagcgccgc
     7801 aacaccgttc accgcgaaac cgccaaccac acacaggttc gccatatgca cttgtttatc
     7861 gtgcaccacc gccagtttgg cccacacttt ttcatcgccc ggccaggttt tctccaccag
     7921 cgttttaaag cgagtattaa tttcgttaat aatctgcata tggcgcggca gtaagccttt
     7981 caccagtttc acatcccagc gttccagcgc ttctggcatc agggtatggt tggtgtaagc
     8041 gaaagttttg ctggtgatgg cccaggcgtc atcccagctc atctggtgct catcggtcag
     8101 cacgcgcagc agttctggaa tcgcgatagt tggatgggta tcgttcagct gaataacttc
     8161 gtaatccgcc agttcgtgca gtttacgccc cgccagatga tggcgacgca aaatatccgc
     8221 taccgaacag gcacactgga agtattgctg catcaggcgc agctttttac cggcagtatg
     8281 gttgtcgttt ggatagagaa ctttggtcag tttttccgca ttgatgcctt gctgttcggc
     8341 gcgcaggaaa tcaccgtcgt taaatttagt tagatcaaac ggatgcgcat gcgtcgcctg
     8401 ccacagacgc agcggctgcg ccacgccatt acgatagccg acaacgggga gatcccacgc
     8461 ttgaccggta atggtaaact ccggctccca gcgtccgtct ttcatcactt taccgccaat
     8521 ccctacctgc acatccagtg cttcgttgtg gcggaaccac gggtagttac tgcgatgcca
     8581 gtcatccggc gcttcaacct gtttgccatc gacaaaagac tggcggaaca aaccatattg
     8641 atagttcaga ccgtaacccg tcgcagactg accgacagtt gccattgagt cgaggaagca
     8701 cgccgccaga cgtcccagac caccgttacc cagcgccggg tcgatctctt cttccagcag
     8761 gtcagtcagg ttgatgtcat aagccttcaa cgaatcctgt acatcctgat accagccgag
     8821 attcaacagg ttgttgcccg tcagacgacc aatcaaaaac tccattgaga tgtagttaac
     8881 atgtcgctga ttcgccaccg gtttggcgaa tggctgagca cgcagcattt cggccagtgc
     8941 ttcactcact gccagccacc actggcgagg agtcatttca gccgcagaat ttaagccata
     9001 acgctgccac tgacgtgaaa gcgcttcctg aaattgctta tcgttaaaaa taggttgtga
     9061 cataggagtt ccacttttct tagattttca acacaacgtt atcgctagtt tgccaggctc
     9121 gatgttgacc ttcctcatcc tgcgggggat taggcaggga ggagttgcgg ggatgagcaa
     9181 ggaaatgtga tctcgaccac ttaaagctag tgcaaaccac aggattagca gcaaatcaat
     9241 gccatacagc gcagaaaatc tgtatctaag tgcaaaaaat ggccgttgcg tattttcaaa
     9301 aagcggaagg taactctata aattaagtaa aggagtgaaa cagtttcaca agtaaaatat
     9361 ccagtgtgct ccatctcatt cttaatagat ttattaagat catcttttta gatggcactt
     9421 tcatcaggaa tgaagaagaa acccttgctt aaatgaatct gatgaacata agggaaacca
     9481 gtattcacgc tggatcagcg tcgttttagg tgagttgtta ataaagattt ggaattgtga
     9541 cacagtgcaa attcagacac ataaaaaaac gtcatcgctt gcattagaaa ggtttctggc
     9601 cgaccttata accattaatt acgaagcgca aaaaaaataa tatttcctca ttttccacag
     9661 tgaagtgatt aactatgctg attccgtcaa aactaagtcg tccggttcga ctcgaccata
     9721 ccgtggttcg tgagcgcctg ctggctaaac tttccggcgc gaacaacttc cggctggcgc
     9781 tgatcacgag tcctgcgggc tacggaaaga ccaccctcat ttcccagtgg gcggcaggca
     9841 aaaacgatat cggctggtac tcgctggatg aaggtgataa ccagcaagag cgtttcgcca
     9901 gctatctcat tgccgccgtg cagcaggcaa ccaacggtca ctgtgcgata tgtgagacga
     9961 tggagcaaaa acggcaatat gccagcctga cgtcactctt cgcccagctt ttcattgagc
    10021 tggcggaatg gcatagccca ctttatctgg tcatcgatga ctatcatctg atcactaatc
    10081 cagtgatcca cgagtcaatg cgcttcttta ttcgccatca accagaaaat ctcaccctgg
    10141 tggtgttgtc acgcaacctt ccgcaactgg gcattgccaa tctgcgtgtt cgtgaccaac
    10201 tgctggaaat tggcagtcag caactggcat ttacccatca ggaagcgaag cagttttttg
    10261 attgccgtct gtcatcgccg attgaagccg cagaaagcag tcggatttgc gatgacgttt
    10321 ccggttgggc gacggcacta tagctaatcg ccctctccgc ccggcagaat acccactcag
    10381 cccataagtc ggcacgccgc ctggcgggaa tcaatgccag ccatctttcg gattatctgg
    10441 tcgatgaggt tttggataac gtcgatctcg caacgcgcca ttttctgttg aaaagcgcca
    10501 ttttgcgctc aatgaacgat gccctcatca cccgtgtgac cggcgaagaa aacgggcaaa
    10561 tgcgtctcga agagattgag cgtcaggggc tgtttttaca gcggatggat gataccggcg
    10621 agtggttctg ctatcacccg ctgtttggca acttcctgcg ccagcgctgc cagtgggaac
    10681 tggcggcgga gctgccggaa atccaccgtg ccgccgcaga aagctggatg gcccagggat
    10741 ttcccagcga agcgattcat catgcgctgg cggcaggcga tgcgctgatg ctgcgcgata
    10801 ttctgcttaa tcacgcctgg agtttgttca accatagcga actgtcgctg ctggaagagt
    10861 cgcttaaggc cctgccgtgg gacagtttgc tggaaaatcc gcagttagtg ttattgcagg
    10921 cgtggctaat gcaaagccaa catcgctatg gcgaagttaa caccctgcta gcccgtgctg
    10981 aacatgaaat caaggacatc agagaaggca ccatgcatgc agaatttaac gctctgcgcg
    11041 cccaggtggc gattaacgat ggtaatcagg atgaagcgga acggctggca aaactggcac
    11101 tggaagagct gccgccgggc tggttctata gccgcattgt ggcaacctcg gtgctgggtg
    11161 aagtgctgca ctgcaaaggc gaattgaccc gctcactggc gctagtgcag caaaccgaac
    11221 agatggcacg ccagcacgac gtctggcact acgccttatg gagtttaatt cagcaaagtg
    11281 aaattctgtt tgcccaaggg ttcctgcaaa ccgcgtggga aacgcaggaa aaagcgttcc
    11341 agctgatcaa cgagcagcat ctggaacagc tgccaatgca tgagtttctg gtgcgcatcc
    11401 gagcacaact gttatgggcc tgggcacggc tggatgaagc cgaagcgtcg gcgcgtagcg
    11461 ggattgaagt cttgtcgtct tatcagccac agcaacagct tcagtgcctg gcaatgttga
    11521 ttcaatgctc gctggcccgt ggtgatttag ataacgcccg tagccagttg aaccgtctgg
    11581 aaaacctgct ggggaatggc aaatatcaca gcgactggat ctctaacgcc aacaaagtcc
    11641 gggtgattta ctggcaaatg accggcgata aagccgccgc tgccaactgg ttgcgtcata
    11701 cggctaaacc ggagtttgcg aacaaccact tcctgcaagg tcaatggcgc aacattgccc
    11761 gcgcacaaat cttgctgggc gagtttgaac ctgcggaaat tgttctcgaa gaactcaatg
    11821 aaaatgcccg cagtctgcgg ttgatgagcg atctcaaccg taacctgttg ctgcttaatc
    11881 aactgtactg gcaggccgga cgtaaaagtg acgcccagcg cgtgttgctg gacgcattaa
    11941 aactggcgaa tcgcaccgga tttatcagtc attttgtcat cgaaggcgaa gcgatggcgc
    12001 aacaactgcg ccagctgatt caacttaata cgctgccgga actggaacag catcgcgcgc
    12061 agcgtattct gcgagaaatc aatcaacatc atcggcataa attcgcccat ttcgatgaga
    12121 atttcgttga acgtctgcta aatcatccgg aagtgccaga actaatccgc accagcccgt
    12181 tgacccaacg tgaatggcag gtcctggggc tgatctactc cggttacagc aatgaacaaa
    12241 ttgcgggcga actggaagtg gcggcaacca ccatcaagac gcatatccgc aatctgtacc
    12301 aaaaactcgg cgtcgcccac cgtcaggctg ccgtgcagca cgcgcagaaa ctgctgaaga
    12361 tgatgggtta tggggtgtga gtttagccgg ataatgcatt ggatccggtt tacatcttta
    12421 catcattcaa tactcacccg cgttacgcca tctgtttcta tcaaaccaaa ccgcaccggc
    12481 aagaaacgct ccaccaccgc gatattggtc agcagatggc atgaaggatg ggcgaccgta
    12541 aattctcccg cgcccgccag cgccatcggc agtaccagtt ggtcggcgag atattccccc
    12601 accgccgctg ggcttgccag gtagcgtttc acctctttca ctaactgcgc ggctaccacc
    12661 tcggcactga cgcgcttttc accgacgacg aaaaagcgtt cagtgatatt ttcactttcg
    12721 acttccagcg agacggtatt acccggcccc tggtcgcgcg gcaggttatg aatattctgt
    12781 tcatgcaggg aaaaaatccc cgccagtgta gcgatttcac gctcagcaac atggcgcggc
    12841 acaccagcta atagaacttc tccacgcatc tgcacaatgt tcccgcgctc gccaagttgc
    12901 aaggtgctaa acgatgccac gggcgagact tccgttgcca ccacaccgcc tccggcagga
    12961 taaaaaccgt gacgtaacag cgtggtttgc tgatgaattc ctattttcgc cagcagcggc
    13021 tccagcaccc ggcggataaa atccgcaggc ggcgccgacg ggttatcggt gccgccgctc
    13081 acttcaacac gcgaaggtcc atcggcaaac cacagcgcgg gcagcaccgt ttgcagcacc
    13141 agcgtacaac ttccggcgct accgatagca aagcggtaat cgccgccgcg cacggtgcca
    13201 ggccggaaga ccagacgctg cgatcccagc tccgcgcctt ccaccgttgc cctacaaatt
    13261 tccgcagccg cttttaccgc ggtcagatgc tggcgcagca gccccggttt cgcccgtccg
    13321 gctcgaatgc cagtgatggt aaatggctgg ccggttatca tcgacaggct cagcgccgag
    13381 cgcaggatct gcccgccgcc ttcgccctgt gcaccatcca gcgcaatcat ccttttcatc
    13441 atttatcctt ttacgcacac cacctgacgc agggtgtaga taacttccac cagatcgctt
    13501 tgtgccgcca tcaccgcatc aatatcttta tacgccatcg ggatttcgtc gatcacttcg
    13561 gcatctttac ggcattccac atgcgcggtg gcacgaattt ggtcttccac gctgaacagt
    13621 tttttcgctt tagttcggct cattacccgc ccggcaccgt ggctgcacga acagaacgac
    13681 tcttcatttc ccagcccacg gacgataaag ctttttgctc ccatcgaacc gggaataatt
    13741 ccatattgac cagcacgcgc agacaccgca cctttacgcg tcacgtagat ctcttcacca
    13801 aagagctgtt ctttttgcac atagttgtgg tgacagttga tctcttccat cgccagcgtt
    13861 tgtggctgtc tgaccgtttt ctgcgtaatg ctctgcaacg ccgttaccac attttccatc
    13921 atcgcatcgc ggttaaggct ggcgaaaagc tgcgcccagg ccacggctct taggtaatca
    13981 tcaaagtatt ccgtaccttc cataaagtac gccagatcac gcgacggcaa cgtctcaagt
    14041 tggtcctgca tctctttttg cgccagatcg ataaagtaag tcccgatggc gttaccaatt
    14101 ccgcgtgaac cggagtgcag cataatccac acctgctccg actcatcaag gcagatttca
    14161 ataaagtggt tacccgttcc cagcgttccc aggtgtttat agttgttagt gttaaggaaa
    14221 cgcggatatt tttgcgttaa ccactgataa ccggcctcca gctcagccca tttcgcatcg
    14281 acgttaaaag gcgggttttc ccatgcgcct ttatcacgtt tacaacgccc ggtagtacgc
    14341 ccgtgtggta cggccgtttc aatcgcttga cgcagctccg ccaggttttc aggcaggtct
    14401 gccgccgtta acgcggtacg cagcgcgttc attccacagc caatatccac gcccaccgcc
    14461 gccggaataa tcgccccttt ggtcgggatc acgctaccaa tggtggaacc tttacccagg
    14521 tgtacatcag gcattaccgc aatatgtttg aaaataaacg gcatcttcgc cgtattaata
    14581 agttgctgac gcgcatcggc ttctaccggc acgcctttgg tccacatttt taccggggca
    14641 ttttcagtgg tcagtaattc gtaattcatt ttgtttttct cttttcgttg tttgcggtcc
    14701 tgataattgc aaccgtcgtg ccagaaaatt aaataagcag attaattttt taatttattg
    14761 tttctaaaaa gattatatct ttatgtccgt aaacggagat ttcccgcaaa gccgatttac
    14821 cgataatgaa atatcgcctt ttataaggat atctaagatg cgtaaaacag tggcttttgg
    14881 ctttgtcggt accgtgctgg attatgccgg acgcggcagt cagcgctggt caaaatggcg
    14941 tccgtcactc tgtatatacc agcaagaatc gttggtcatc gatcgactgg aattattgca
    15001 cgatgcccgc tcgcgctcgc tatttgaaac gcttaaacgc gatatcgcca gcgtttcgcc
    15061 agaaacagaa gtggtgggcg ttgagattga actgcataac ccgtgggatt tcgaagaggt
    15121 ttacgcctgc ctgcatgatt tcgcccgtgg ttacgagttt cagccagaaa aagaagacta
    15181 tttaattcac atcaccaccg gcacccacgt cgcgcagatt tgctggtttc tgctggcgga
    15241 agcgcgttat ctacccgccc ggttgataca atcttcaact ccgcgcaaaa aagaacagcc
    15301 gcgcggccca ggtgaagtga cgattatcga tctcgattta agccgttata acgccatcgc
    15361 cagccgcttt gccgaggaac ggcagcaaac gctcgatttc ctcaaatccg gtattgccac
    15421 gcgtaacccc cacttcaacc gcatgattga gcagatcgaa aaagtggcga ttaaatcccg
    15481 cgcgccgatt ctgcttaacg gcccgaccgg cgcaggtaag tcatttctgg cgcgacgcat
    15541 cttcgagtta aaacaggcac ggcatcagtt tagcggcgcg tttgtggaag tgaactgcgc
    15601 caccctgcgc ggcgataccg cgatgtcgac gctgtttggt catgtgaaag gcgcgtttac
    15661 cggggcgcgg gaatctcgtg aaggattatt acgcagcgcc aacggcggaa tgttgtttct
    15721 tgatgagatt ggcgaactgg gcgcagacga acaggcaatg ctgctgaaag ctattgaaga
    15781 gaaaaccttt tacccgtttg gcagcgatcg ccaggtgagc agtgattttc agcttatcgc
    15841 cggaacggtg cgcgatttgc gccagctggt tgccgaaggc aaattccgcg aagatctgta
    15901 cgcgcggatc aatctctgga ccttcaccct gccgggtcta cgccagcgcc aggaagatat
    15961 tgaaccgaac ctggattatg aagtggagcg ccacgccaca ctcactggtg acagcgtgcg
    16021 ttttaacacc gaagcgcggc gtgcctggct ggcctttgca acatctcccc aggcaacatg
    16081 gcgcggtaac tttcgcgaac tttctgccag cgtcacgcgg atggccacct ttgccactag
    16141 cggacgcatc actctggacg tggtggaaga tgagataaac cgtctgcgct ataactggca
    16201 ggagagccgc ccctccgcgc ttacggcgtt gctgggcgca gaggcagaaa acatcgatct
    16261 cttcgaccgt atgcaactgg aacacgttat cgctatctgc cgccaggcaa agtcgctttc
    16321 tgctgccgga cgacagcttt ttgatgtttc gcgccagggc aaagccagcg tcaatgacgc
    16381 ggatcggcta cgcaaatacc tggcgcgttt tggtctgacg tgggaagccg tgcaggatca
    16441 gcacagctcc agttgaatat ggtggtccgt cagcacctgc atcacgctta ctggcggcgg
    16501 ggcgtcggtg tagacggcat caaccatgct gatgctcccc atattgacca tcgcattacg
    16561 gccaaatttt gaatggtcaa ccaccagcat aacgtggcgc gagttctcaa taatggcgcg
    16621 cttggtacga acttcgtggt aatcgaactc cagcagcgag ccgtcgctgt cgatgccgct
    16681 gatccccaga atgccgaaat caaggcggaa ctgggagata aaatcgagcg tcgcttcgcc
    16741 aatgatcccg ccatcgcggc tacgtaattc gccaccggcg agaataatgc ggaaatcttc
    16801 ttttaccatc aacgtgttag caacgttgag attgttggtg acaatgcgca aattgctgtg
    16861 attgagcagc gcgtgcgcca ccgcttccgg cgtggtgccg atatcgataa acagcgtcga
    16921 gccattgggg atttgctccg ccactttgcg ggcgatgcgc tctttttctt cggtctgggt
    16981 ggctttacga tcgtgccacg gcgtgttaac cgaactggaa ggcagcgccg cgccgccatg
    17041 atggcgcagg atcaggtttt gctctgccag ctcgttgagg tcgcggcgaa tagtctgcgg
    17101 gctgacggag aagtgctcca ccagttcctc ggtactgaca taaccctgct gtttaaccag
    17161 ttcgataata ccgttgtgac gttgtgtttg tttcatttat aaatccctgg aattattttc
    17221 gttttcgcgc attgagcgaa tcaacaaaag ccatcgctaa acccacggct aatccggcga
    17281 tgtgtgctcc gttcgccatc gacatcccaa acaaatcaaa ccatccggcg acaatccaga
    17341 tcagcgcaaa gataattaac ccacgttgca ggtaaatgcc actttgcgga tcgcgttcgc
    17401 cacgtagcca gacgtagccc atcagcgcat acaccacgcc agaaagcccg ccaaaccacg
    17461 gcccgctgaa tttttgctgc atatagccgc ttaacagggc gctgataagc gtaatgacaa
    17521 ttagcttacc gctaccgagg cgtttttcca ccgcgccgcc gagataccac caccaaagca
    17581 ggttaaagag gatatgcatc agcgagaagt gcattaacgc gtgggtgaag taacgccaga
    17641 actcaaattt cagtgttgga tcgaatggcc aggccagcca taacatcact tcctgatcgc
    17701 cgagaatttg catggcaata aacaccacca cgcaggcgat catcatcacc caggtcaccg
    17761 gacctgcgcg ttcacgcaag gcagcaaaga aaggataacg gcgataatgc aggccactgc
    17821 cggtatggcc tgccagccag ctggccgcca gataacgcgg atctgccggg ttttcgagaa
    17881 aacgcgccag ctccgcccgc acgcgctcgg cctgggactc atccgccagc cagacatcgc
    17941 tttggttatg ttgttgaatc gtgaggataa caccctgcgt cgccatgtaa tcaacaaacg
    18001 cctgcgccac gcgggggtta gcaaaagagg taatcatcaa catcgttgct gtcgcttatt
    18061 ccacacaaaa ggggacagta taaagcgtta cgcgccgtac gccacctctg cgggaaactg
    18121 tcgctgccag gcttcaaagc cgccgtcaat gctatagacc acatcgtagc cctgttgcag
    18181 cagatactgc gccgcccctt tgctgctatt gccgtgataa cacatcacca tcaccggagt
    18241 gtcaaagtcg ttatcacgca taaacgcgcc cagcgtgtcg ttggttaaat ggaaagcctg
    18301 caccgcatgc cccatagcga aactctgtgg atcgcgaata tcgaccagca ccgcctcttt
    18361 ttcctgcaac ttctggtgcg cgtcggcaac gttaatacat tcgaactgat ccatgcgtct
    18421 ctctttcttt acaaacaagt gggcaaattt accgcacagt ttacgtcgaa gcggcagata
    18481 aacgccataa tgttatacat atcactctaa aatgtttttt caatgttacc caaagcgcga
    18541 ttctttgcta atatgttcga taacgaacat ttatgagctt taacgaaagt gaatgagggc
    18601 agcatggaaa ccaaagatct gattgtgata ggtggcggca tcaatggtgc tggtatcgcg
    18661 gcagacgccg ctggacgcgg tttatccgtg ctgatgctgg aggcgcagga tctcgcttgc
    18721 gcgacctctt ccgccagttc aaaactcatt cacggtggcc tgcgctacct tgagcactat
    18781 gaattccgcc tggtcagcga ggcgctggct gaacgtgaag tgctgctgaa aatggctccg
    18841 catatcgcct tcccgatgcg ttttcgcctg ccacatcgtc cgcatctgcg cccggcgtgg
    18901 atgattcgca ttggtctgtt tatgtacgat catctgggta aacgcaccag cctgccggga
    18961 tcaactggtt tgcgttttgg cgcaaattca gtgttaaaac cggaaattaa gcgcggattc
    19021 gaatattctg actgttgggt agacgacgcc cgtctggtac tcgccaacgc ccagatggtg
    19081 gtgcgtaaag gcggcgaagt gcttactcgg actcgcgcca cctctgctcg ccgcgaaaac
    19141 ggcctgtgga ttgtggaagc ggaagatatc gataccggca aaaaatatag ctggcaagcg
    19201 cgcggcctgg ttaacgccac cggcccgtgg gtgaaacagt tcttcgacga ggggatgcat
    19261 ctgccttcgc cttatggcat tcgcctgatc aaaggcagcc atattgtggt gccgcgcgtg
    19321 catacccaaa agcaggccta cattctgcaa aacgaagata aacgcattgt gttcgtgatc
    19381 ccgtggatgg acgagttttc catcatcggc accaccgatg tggaatacaa aggtgatccg
    19441 aaagcagtga agattgaaga gagtgaaatc aattacctgc tgaaagtgta taacgcgcac
    19501 tttaaaaagc agctaagccg tgacgatatc gtctggacct actccggtgt gcgtccgctg
    19561 tgtgatgatg agtccgactc gccgcaagcc attacccgtg attacaccct tgatatccat
    19621 gatgaaaacg gcaaagcacc gctgctgtcg gtattcggcg gtaagctgac cacctaccga
    19681 aaactggcag aacatgcgct ggaaaaactg acgccgtatt atcagggtat tggcccggcg
    19741 tggacgaaag agagcgtgct gccgggtggt gccattgaag gcgaccgcga cgattacgcc
    19801 gctcgcctgc gccgccgtta cccgttcctg actgaatcgc tggcacgtca ctacacgcgc
    19861 acttacggca gcaacagcga gctgctgctc ggcaatgcgg gaacggtaag cgatctcggg
    19921 gaagatttcg gtcatgagtt ctacgaagcg gaactgaaat acctggtgga tcacgaatgg
    19981 gtccgccgca ccgacgatgc cctgtggcgt cgcacaaaac aaggcatgtg gctgaatgcg
    20041 gatcaacaat ctcgtgtgag tcagtggctg gtggaatata cgcagcagaa gttgtcgctg
    20101 gcgtcgtaaa ttaacgtaag gtgatcaggt cagatttcaa tctggcctga gactgatgac
    20161 aaacacaaaa ctgcctgatg cgctacgctt atcaggccta cgtggtttat gcaatatatt
    20221 gaatttgcat ggtcttgtag gccagataag acgttcacgt cgcatccggc atgaacaaag
    20281 cgcactttgt caaaaatcta acctactttt taattcaggg aattaccgca aagcccacgc
    20341 catcataccc aacgtaacaa aactcaggca cgttccccac tcgccccgag aaaaatagca
    20401 ttaatgcgcc cagcgtcagc ataaaaatat tgagcagaag gtaaattgtt ggcgtaaaga
    20461 gtaaattgca aacctcagga atagcggcaa gcccagcccc cagcatggca ataaccgctg
    20521 caaaaaattg cgtcagtatt gggatgccga aaaaagtcat taccagcgag gtcaaaatcc
    20581 attttctatt ttgcattatt ctttccattc tttctgaatg gcgaaattat actccccgag
    20641 tccccttgcc ccttctggac acttttccga aatgatggcg aaaaaaacgg g
//
LOCUS       DAJTQY010000069        20670 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.069, whole genome shotgun sequence.
ACCESSION   DAJTQY010000069 DAJTQY010000000
VERSION     DAJTQY010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20670
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.069"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            <1..574
                     /locus_tag="OO349_002647"
     CDS             <1..574
                     /locus_tag="OO349_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR5867667.1"
                     /translation="AINAALTEKRIKAVVSITGVNIGRLFREGFSNYDPIGVLNAMAS
                     QRAKEARGGELQINELLPASLDAAKAHGLTERDVYEATDYYKTPRGQQPGGATKMLFS
                     HAQKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRDGLEVYGRAMVSQDRQLVSLP
                     DFSHYELYDKPEAVQEALAKVIPFFNTHLG"
     gene            592..1230
                     /locus_tag="OO349_002648"
     CDS             592..1230
                     /locus_tag="OO349_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5867668.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSQIIMGDVLNHAALKQAMQGQDIVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFVEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPGTDGDKPFFM"
     gene            1521..2249
                     /gene="mpaA"
                     /locus_tag="OO349_002649"
     CDS             1521..2249
                     /gene="mpaA"
                     /locus_tag="OO349_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HCR5867669.1"
                     /translation="MTVTRSRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQSFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(2224..3183)
                     /gene="ycjG"
                     /locus_tag="OO349_002650"
     CDS             complement(2224..3183)
                     /gene="ycjG"
                     /locus_tag="OO349_002650"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HCR5867670.1"
                     /translation="MRIVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALATEARAQGFSLMLGCMLC
                     TSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            3302..3808
                     /gene="tpx"
                     /locus_tag="OO349_002651"
     CDS             3302..3808
                     /gene="tpx"
                     /locus_tag="OO349_002651"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HCR5867671.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(3852..5393)
                     /gene="tyrR"
                     /locus_tag="OO349_002652"
     CDS             complement(3852..5393)
                     /gene="tyrR"
                     /locus_tag="OO349_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HCR5867672.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(5541..6602)
                     /locus_tag="OO349_002653"
     CDS             complement(5541..6602)
                     /locus_tag="OO349_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HCR5867673.1"
                     /translation="MTEPLKPRIDFDGPLEVEQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGIQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISHSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTVRLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(6599..7996)
                     /locus_tag="OO349_002654"
     CDS             complement(6599..7996)
                     /locus_tag="OO349_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HCR5867674.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAVWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWDESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AAMKADHVTVDQHANMVSLLQQLIQDAWQNAAFERISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            8152..9150
                     /locus_tag="OO349_002655"
     CDS             8152..9150
                     /locus_tag="OO349_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR5867675.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADKSFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLSAHNIAVRD
                     KWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(9261..10133)
                     /gene="ompG"
                     /locus_tag="OO349_002656"
                     /pseudo
     CDS             complement(9261..10133)
                     /gene="ompG"
                     /locus_tag="OO349_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006175591.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
     gene            complement(10206..11287)
                     /locus_tag="OO349_002657"
                     /pseudo
     CDS             complement(10206..11287)
                     /locus_tag="OO349_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(11301..11960)
                     /gene="pgmB"
                     /locus_tag="OO349_002658"
     CDS             complement(11301..11960)
                     /gene="pgmB"
                     /locus_tag="OO349_002658"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HCR5867676.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIRNLL
                     AELRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(11957..14224)
                     /gene="ycjT"
                     /locus_tag="OO349_002659"
     CDS             complement(11957..14224)
                     /gene="ycjT"
                     /locus_tag="OO349_002659"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HCR5867677.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGAGYTIASRRFVSADQLPLIALEITIMPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCQVSGDVQQCYTAKERRLLQHTS
                     TQLHAGETVTLQKLVWIDWRDDRQAALDEWGSVSLRQLEMCAQQSYDQHLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHGERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMVYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMTKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSSASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LLEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            complement(14221..14844)
                     /locus_tag="OO349_002660"
     CDS             complement(14221..14844)
                     /locus_tag="OO349_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR5867678.1"
                     /translation="MTNGVLGEIYVTTARALRRCGVPGWGVFTNKELQGGGLLIDIGI
                     HMLDAAMYVLGFPAVKSVNAHSFQKIGTQKSCGQFGEWDPATYSVEDSLFGTIEFHNG
                     GILWLETSFALNIREQLIMNVSFCGDKAGATLFPAHIYTDNNGELMTLMQREMADDNR
                     HLRSMEAFINHVQGKPVMIADAEQGYIIQQLVAALYQSAETGTRVEL"
     gene            complement(14841..15263)
                     /locus_tag="OO349_002661"
     CDS             complement(14841..15263)
                     /locus_tag="OO349_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005084158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR5867679.1"
                     /translation="MTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSRLSQA
                     QALAEKYGNASVWDDPQAMLLAVKPDVVSVGSPNRFHYEHTLMALEAGCHVMCEKPPA
                     MTTEQAWEMCDTARKLGKVLAYDFHHRLRSIRNSCVNR"
     gene            complement(15285..16060)
                     /locus_tag="OO349_002662"
                     /pseudo
     CDS             complement(15285..16060)
                     /locus_tag="OO349_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
     gene            complement(16078..17130)
                     /locus_tag="OO349_002663"
     CDS             complement(16078..17130)
                     /locus_tag="OO349_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HCR5867680.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPQGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(17161..18003)
                     /locus_tag="OO349_002664"
     CDS             complement(17161..18003)
                     /locus_tag="OO349_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR5867681.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            complement(17990..18871)
                     /locus_tag="OO349_002665"
     CDS             complement(17990..18871)
                     /locus_tag="OO349_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR5867682.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(18892..20175)
                     /locus_tag="OO349_002666"
                     /pseudo
     CDS             complement(18892..20175)
                     /locus_tag="OO349_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
     gene            complement(20189..>20670)
                     /gene="ycjM"
                     /locus_tag="OO349_002667"
     CDS             complement(20189..>20670)
                     /gene="ycjM"
                     /locus_tag="OO349_002667"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HCR5867683.1"
                     /translation="RCTRFILAHAILLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRA
                     INRKKYHGEEITRELNDEATLRHAVYHELSRLITLRRSHNEFHPDNNFTIDTVNSSVM
                     RIQRSNADGNCLTGLFNVSKNIQHVNITDLHGRDLISEVDILGNEITLRPWQVMWIK"
BASE COUNT         5048 a         5636 c         5030 g         4956 t
ORIGIN      
        1 tgccattaat gccgcgctga cagaaaaacg aattaaagct gtagtgagta ttacgggtgt
       61 taatatcggg cgtctttttc gggaaggttt cagcaattat gatccgattg gcgtattgaa
      121 tgcaatggcg tcacaacgag caaaagaggc tcgtggtgga gaattacaga tcaacgaatt
      181 acttcccgcc agcctggatg cagcaaaagc ccatggactg accgaacgcg atgtttatga
      241 agcaactgac tactataaaa caccacgcgg gcaacaaccg ggaggtgcga caaaaatgtt
      301 gttctcacat gcacagaaaa cgctggcctg ggatgccttc gcatttactg aagtgttact
      361 gacccagccg gtgatggtag tagttggtga aaaagtcggt gccttcggtg cttatcgtga
      421 tggactggaa gtctatggcc gggcgatggt ttcgcaggat cgtcagttag tttcgttacc
      481 cgatttctca cattatgaac tatatgacaa accggaagca gttcaggagg cgctggcaaa
      541 agttattcct tttttcaaca cccatctggg ataagtttca tgagaaatat tatgaaaaat
      601 gtactgattc ttggtgctgg cggtcagata gcccgccatg tgattaatca actggcagat
      661 aaacaaacta ttaaacaaac tttatttgcc cgtcagccag caaaaataca caaaccctat
      721 ccaactaata gccagattat tatgggggat gtactgaatc atgcagcact gaagcaggcc
      781 atgcagggac aggatattgt gtatgccaac cttacggggg aagatttaga tattcaggca
      841 aattcagtga tcgctgcgat gaaagcctgt gatgttaaac gtctgatctt tgttctttcc
      901 ctcggtattt atgatgaagt tccaggaaaa tttgtcgaat ggaataatgc agttattggt
      961 gaacccctaa aaccattccg cagggctgcg gatgcaattg aggcttctgg actggaatat
     1021 acaattcttc gccctgcctg gctgactgat gaagatatta ttgattatga gcttacctct
     1081 cgcaatgaac cattcaaagg tacgatcgta tcacgtaaga gtgtcgcagc attaattaca
     1141 gatatcatcg ataaacctga gaaacacatt ggtgagaata taggcatcaa tcaacccggg
     1201 actgatggcg ataagccatt cttcatgtaa atcaattata tagataccat cgtactgtac
     1261 tatgtgcagt acgatttatt aaacttatta aggtgaactg atcaatcaga tgcgattaac
     1321 atgtgtagaa ccagtaagaa taaaatacgc catgtatata acattacgac aattctaata
     1381 tctgcacatc aatgaaaatg caaacaactt ccggcaaaaa agtgatacaa gatcgatgac
     1441 atagcgttga agaaaaggat ttgataatcc gcaaaatttg cacaacactg cctgtaatac
     1501 ttcctgaacc agagacgatc atgaccgtaa cccgctcacg cgccgaacgc ggcgcatttc
     1561 cgcccggaac agaacattac ggacgttcat tattgggcgc gccgttaatc tggtttcctg
     1621 cccctgcagc cagccgcgaa agtggtttga ttctggctgg cactcacggt gatgaaaact
     1681 cttcggtcgt cacgctctcc tgtgctctgc ggactttgac accttctttg cgccgccatc
     1741 atgtggtgct gtgtgtgaat cctgacggct gccagctggg gttacgggcc aatgctaatg
     1801 gtgtggattt aaacagaaac tttccggcgg cgaactggaa ggaaggtgaa acggtttatc
     1861 gctggaacag cgccgctgaa gaacgtgatg tcgtattact gacaggtgac aaacctggct
     1921 ccgaacctga aacccaggcg ctgtgccagc ttatacaccg cattcaacct gcctgggtgg
     1981 tctcattcca cgatccactg gcttgtattg aagatcccag acacagcgaa ttaggcgaat
     2041 ggctggccca gtcgtttgaa ttgcctctgg taaccagcgt cggttatgaa acgcctggct
     2101 ctttcggcag ttggtgtgca gatctcaact tgcattgcat caccgcagag tttccgccca
     2161 tctcctccga cgaagccagc gaaaaatatc tctttgcgat ggcaaacttg ctgcgctggc
     2221 atcctaaaga tgcaattcgc ccgtcgtgaa ctgaagcgcc ggttccacat ctaccgccag
     2281 ccaggtcggt ccgtcaagat cggcgaaact gacctgcggc accagcggta aagcggcgct
     2341 aatggcacga gaggtacaca acatgcagcc cagcatcaga ctgaaacctt gtgcacgcgc
     2401 ttcagtcgcc agcgcttccg tcagaccacc ggttttatcg agcttaatgt taaccatctc
     2461 atagcgccct ttcagcgcct tcagattgct acgagtatga caactttcat cagcacaaat
     2521 cggcaacgga tgaataaaat tctccagcgc cgcatcgtcc tgcgcaggaa gcggttgttc
     2581 aagcatcgca acgcctaaat ccgccaatag ctggcaacgc gccgccaacc cttctgcacg
     2641 ccaggattca tttgcatcaa cgatcagcgt ggcatcgggc acagcggtgc gaattgccac
     2701 catccgctca ctgataagat ggttatccag cttcactttc agtaatttcg cgcctgcctg
     2761 ccagagtgtt gatgcactat tggccatctg atcaggcgta ccgatgacaa ccgtctgtgc
     2821 agtgataact gtctcgggaa gcgttatgcc gatcaaatca gccagcgatt gctgctgttt
     2881 tcgcgccgcg agatcccaca atgcacaatc cagcgcatta cgtgctgcgc cggcagggag
     2941 aattttttgc aactcctccc gtgtcagccc tttctctaat tgcggcacga cgctcataat
     3001 ttgcgccatt accgaggcat cactttctcc ataacgcgga tacggcgtgc attcgccggt
     3061 gcctttaata ccctcttctt ccagttcaac caccaccacg cgcgcttcac tgcgacttcc
     3121 ccgggcaatc acaaacgggg tatgtaaggg ccaggcttcc tcaaatacct taacgattct
     3181 catctttact ccttgttgtc gcggtggtct gcggattaag cgcaaatagc gtttgctgtg
     3241 ttattgacag ttagcataaa ctaggtgtga cgttaactat atgtaaacag gaaagataat
     3301 catgtcacaa accgttcatt tccagggcaa cccggttaca gtcgccaatt ccatcccgca
     3361 ggcgggtagc aaagcgcaga cttttactct cgtggcaaaa gatctgtctg acgtcaccct
     3421 cggtcagttt gcgggtaaac gcaaagtgct gaacattttc ccgagtattg ataccggtgt
     3481 ttgcgccgca tcagtacgta agtttaacca actggcaact gagatcgaca acaccgttgt
     3541 gctgtgtatc tctgccgatc tgccgttcgc ccagtctcgt ttctgcggcg cagaaggtct
     3601 gaacaacgtt atcaccctct ccactttccg taacgctgaa tttctgcaag cctacggtgt
     3661 ggcaattgct gatggcccac tgaaaggtct ggcagcgcgt gccgttgtgg ttattgacga
     3721 aaatgacaat gtgattttca gccagctggt ggatgaaatc accaccgagc cggattacga
     3781 agcagctctg gctgtactga aagcataagt taatatgcct gatggtgttg caccatcagg
     3841 catattcgcg cttactcttc gttcttcttc tgactcagac catattcccg caacttattg
     3901 gcaatcgcgg tatgtgaaac gccgagacgt tttgccagtt tgcgcgtgct gggataattg
     3961 cgataaagct gggttaatac cgagcgttca aaacggctgg tgatttcgtc cagcgaacct
     4021 tccatcgcat cttcgcccac ggctaccgtt gcggcgtcat aatccggcaa caaaatatcc
     4081 tgtggacgca gctcataacc gtccagttgt gtcagtgcgc gatagatagc gttctttaac
     4141 tgccgcacat ttcccggcca cgcataacgt gtaagtacag tattcaggtc agcggccagt
     4201 ttcggacgcg gcacgccctg ctcgtcggca aagcgggcga cgaacagctc agttaacggc
     4261 ataatgtcct gcggacagtc acgtagcggc ggcagattga gcgtcaacac gttcagacga
     4321 taatagagat cttcacggaa cacgcctttt tgcaccagtt cgaccagatt cttctgcgta
     4381 gcgcaaatca cccgcacatc gacatgcacc tcatggtctt cgccaacccg acggaaagtg
     4441 ccatcattaa gaaaacgcag taatttcgcc tgcatccgtg gtgacatttc ccctatttca
     4501 tccaacagca ccgaaccacc gttcgcctgc tcaaagaacc ctttcttccc ttccggagca
     4561 tgaccaaaca gttcactctc gaccgcatct tctggtatag acgcgcaatt cagcgccagg
     4621 taaggtttgc ccgctctggg gcttgcctga tggcaggcgt aggcaaaaag atctttacct
     4681 gtacctgtgt cacccgtaat cagcagcggc gcgcttagca tcgccagttt ctgcgcctgt
     4741 tcgacaacgt gcttcatttt cgggctgacg gcgacaattt ggctgaaggc gctgacgtcc
     4801 tgggcggcga cattttgcaa ctggcggccc atacgaatcg ttgatcgcaa catcaccacc
     4861 gcaccggtca ggacgtgttg atcattttca tcctgaagat aaacaggcgt aatctccatc
     4921 aggaaattct gcccattaat aacgacatgc tcgttatgcg aatcttgcgg ttcgctttcc
     4981 agccaacgta aaaaattaaa gccgttaatc aattgtgcgg cggtatggtt gcgcaggcga
     5041 tccaattttt gcccaaaaag ctgacagctc gccgggttcg ccatatccac tttgcttttc
     5101 atatcgacag agagcacagg ttcaggcaac gcctccagta acgcgctcaa cgccagatgc
     5161 tcacgttcgg aaggcatcca cgggacagta cgcacatcgg taacacctgc aatacggcgt
     5221 atttcggcca tcagactgct gaaactctca aactccagtt cagcaaaatt gaggtagatt
     5281 cgcccaatgg gatcaatctc aataccgcgt aaatcaatgc ctcttagcac gagtagatcg
     5341 agtaattcgc gggtcagacc gagtcggtct tcacaaaaga cttccagacg catgggaacc
     5401 ttcacctgaa aaaagaacaa ttaatatgat gatatgacac tatttggata gcaggaaggt
     5461 ttctgtcaac aatcattgac acaaagacgg aaaaacgggc acttcggcgt aaagatatcc
     5521 ggtcagcacc cgatattgca ttatttttcg ctgggcgttt tgcctttttg cagagtttct
     5581 ttcacctgac cgataagctg acgacggaaa tccccaaggc gaggtttgtc atcgtcaatc
     5641 cacggcagcg ggcggcaaag ctccatagct ttaatcccga gtcgtaccgt cagaagtcca
     5701 gcaccaattc cctgagctgc gcgggtagac aaacgagcag cgagatcttg cgacatccag
     5761 tccatcccca cttcgcgcac cagttcgctg gctccggcaa aagcgatatt cagcaatacc
     5821 agcttaaaca gacgcaaacg gctgtaatac cccagttcaa tgccatacag cgtggcaatg
     5881 cgattaatta aacgcagatt gcgccaggcg ataaacgcca tatcgaccaa cgccagcggg
     5941 ctgaccgcaa tcatcaacgt tgattccgcc gccgaatggc tgatttcgcg ccgcgcctgg
     6001 gcatctaaaa ctggctggac caaatgcgca tacaggctga ccacttcacg atcgttctgc
     6061 gtttcatgga ttgaggcata ccagcgttgc agcgccggat gcgactgatc aatacccgcc
     6121 tgctgcgcca gtttttcgca aaatgcgcga cctttgcccg tgccgtggct atgcaataaa
     6181 tcacgcgctt cgtcgcgttc atgggcgcgc tgtcgcaagc gccataagcg ccgccactct
     6241 gttaccacag aacctacgcc agcgccgata atcaatgccc cagcggcaca tccacccagc
     6301 gctacccagt cctgggtttg ccaggcattc attgtccact gtataccctg cccgacaacg
     6361 cttgcgccaa acagagccag cccgcccatc accattttgc gccacaggct gcgtttcgga
     6421 cgtaacgctg cgtccattac cgcttcgact tgcccctctt cttcctgcgc ttcgtcgagc
     6481 gtggccgggg caaaattttg cgcctgattt tcgtcaaagg tctgctgcgc cctgaatttt
     6541 ggattctgtt cgacctccag cggaccgtcg aaatcaatac gtggttttaa cggttcggtc
     6601 atcgcaattt atctcctatt aaaaattcca gcgcggcatc aagacgaata tgcggcagcg
     6661 gtttgtcgac atccatcacc tgcgggcgaa acgcttcaaa ctggaaccct tgcttgtccc
     6721 agaacgcctg accgggcaaa cgcgcaggaa cttcaccggg gtaaacggtg agaggcgcac
     6781 catcgctgag ccggttaccg cgcaatgccg gaattttctc gccgttgacg tcaataatgc
     6841 cgctggtggt cgcctgcacc gaggccagcc ccaggcaatc catactaatc ctttcaaacg
     6901 ccgcattttg ccaggcatcc tgaatcagtt gttgcagtaa tgaaaccata ttggcgtgct
     6961 gatcgaccgt tacatggtcc gctttcatcg cggcaaacag cagtttgtcg ataaccggcg
     7021 agaataagcg gcgaaacagc gtgcgctgcc cgtagtggaa actttgcatc agctgcgtca
     7081 gtgccagacg catatcatta aatgcctgtg gcccactgtt gagaggttgc aggcaatcca
     7141 ccagcacaat ctggcggtca aagcgcagaa aatgattctt atagaacccc ttcaccactt
     7201 tctcgcagta ataattaaac cgctcgcgca gcattccggc attggtgtgc ttatcggcct
     7261 gcgccagttt ggactcgtcc caggcatcga catccggcca cgggaagaat tgcagcgcgg
     7321 gcgcacctgc catatctccc ggcaagacaa agcgcccagg ctgaataaag tgcagcccct
     7381 gctgtttaca gtggtggaga taatcggtcc acacggcggc aatgtctgcc agccggtttt
     7441 cgtcggcagg cgctagcggg tcaagtcctt cgcacatcat tcgccatttc gccgaccatt
     7501 cgccgcgctg accattgagt aagcccgtca tctggcgcga ccagcttaag taatcctgcg
     7561 ccagcatcgg caggtcgagc aaccattcgc cgggataatc cacaatttcc agatacagcg
     7621 tggaggtatc cttaaagtgg cgcagcagcg aatcgttcga tttaaaacgt aacgccaggc
     7681 gaatttcact gacgccgcgc gtaggcgtcg gccaggcggg aggatcacca tataactgcg
     7741 ccagtccttc atcataggtg aagcgcggaa tgccaaagtc acgctgggga atgcgtttta
     7801 cgcccagcaa gcgctcttca cgcaccgcgc ttaacagcgg caaacgtgct ccggcatgaa
     7861 tattgagcaa ctgattaacc atcgcggtga taaacgctgt tttgccgctg cggctaagtc
     7921 cggttacagc gaggcgcaga tgtctgtcga caccccgatt caccagcgca ttgagttcat
     7981 ttttaagtcg cttcattgct gtcctgtgct gctctgaaga ccagaaaaaa aatcattcgt
     8041 aaggatatga atgtgggggc caggccattg attatcaact gcccgtgttg tcatcgacgg
     8101 gcgaactcca taagatgtgc agcattcacg ttcggacgga gttcctgacc tatgtcccct
     8161 actatttatg atattgccag ggttgcaggc gtatcaaaat ccaccgtatc acgcgtgctg
     8221 aataagcaaa ctaatatctc cccggaagcg cgcgaaaaag tgttacgggc cattgaagaa
     8281 ttacagtatc agccaaacaa gctggcccgc gcgctgacct cttcgggttt tgatgccatt
     8341 atggtgattt ctacccgttc gaccaaaact acggcgggta atccgttttt ctctgaagtt
     8401 ttgcatgcca tcaccgccaa agcggaagaa gaaggtttcg acgtgatatt acaaacgtcg
     8461 cacaatccgg cagaagatct gcaaaaatgc gaaagcaaaa ttaaacagaa aatgattaaa
     8521 ggcattatta tgctcagttc gccagcggat aagtcatttt ttgcccaact cgataaatac
     8581 gatattccgg tggtggtaat tggcaaagtt gaaggtcaat atgcccatgt ttattctgtc
     8641 gataccgata attttggtga cagcattgcg ttgaccgatg cattaattga aagtgggcat
     8701 caaaatattg cctgcctgca tgctccgctt gatgtccatg tttcggttga tcgcgttaat
     8761 ggctataagc aaagtctgtc cgcgcataat attgcagtgc gtgataaatg gattgttgac
     8821 ggcggttata cccatgaaac agcactgcaa gcagcacggc aattattaag tcagtcaccg
     8881 ttgcctgagg cagtgtttgc cactgacagc ctgaaattaa tgagcattta tcgtgcggcg
     8941 gcagagaaaa atattgctat tccgcagcag ttagcggtgg tgggttatag taatgaaacg
     9001 ctgtcgttta ttttaacgcc tgcaccaggc ggcatcgatg ttccgacgca ggagttaggt
     9061 caacaaagct gcgagttatt attccgctta atttcgggaa aaccgtcacc acagaatatt
     9121 accgttgcca cgcatatgac gctgaaataa ataccagcta ccttgcctga atgccggatg
     9181 cgatgctggc gcatcttatc cggcctacat ttatagatcg gataaagtac tcgcgccgca
     9241 tccggcaatt tagcccatta tcagaacgag taatttacgc cgacacctgc ataatggaat
     9301 ttatcgctgt cgccttcgtc gtgatcctgc cactcaaacg cgtattccag cgaaacggaa
     9361 agtccgttct ggaaatcata accgtaaaat aaacccacac ggttaaaatc atggccttca
     9421 cgttcaatat catcctgcca gtcccagtta ctctagcgat ccagcccaat gcgcgtatac
     9481 ggcgtcaccg agtggttgcc gagcgtcagc ggcaaatagg cgcgaatttc ttgtgtggaa
     9541 aactcaccgt tattgcggct gtcgtccata ttgaagccgc gctcgagata atagttcact
     9601 cgcaaagcaa ccgtttcgtt gaaggtatat tgcagacctg tttccgtttc gacacgggta
     9661 tcagcgtaac cggtcgtgtt caaatcgttg gcaaatttat acatcgacaa ccaaccgttg
     9721 aaacgtaaat catcagtcag tttcacatcc cagtctggcg cgattttcca gcgctgcata
     9781 ttcgccgtgt ctttgcccgg ttcatcaacg tagtgataac cataattacg gaaaccgccg
     9841 gtcaggccga aactgaaatc atcgctttcg aggaactgat aatgcacctc cagctccggg
     9901 cgatcaaacc tcgttccacg tttacccgcg ctataatcta ccggcccttc ctgataatag
     9961 gccagagcaa ttctccacgg cccgttggcg gcattaaaat agactgaagg ctccgccagc
    10021 ccatccatat cttcgccata accctcgacg ttttctattt cgtacatcgc accgatatta
    10081 aagtgccagt cgctcttttc ctcggcctgt gcgcaggcca ttcccgcaca catcaccagt
    10141 gcggtacagg gtaataactt tttttgttcc ttgtattatc ctgggggaat gccttccccc
    10201 tgtatttagc gaattgctat ttccgtttct gcatcaaaga aatgacattt cgtcatatca
    10261 aaatgaatag tgatattttc tcctgcatga taatcattta acgcaccagc acggaccact
    10321 aactcgtgcc cccaaccgtg gtgtagagca taaattccgc accggttaat tctgccacgc
    10381 taattttggc ggaaatgtta ttttcctctt gcgcgtccgg atgaatatct tccgggcgta
    10441 ttcccatcac gatgggctta tgcaaacttt cctgtgtttt cagaaccgct aatttctctt
    10501 cgagaatggt taatttaagc gtttccgtaa cgaatttatc gccatcgatc gtgccgcgaa
    10561 taaaattcat cgctggtgat ccaataaatc cggcaacaaa catattcgcg ggttggttat
    10621 aaacggtttt cggcgcacct acttgctgaa caatcccgtc tttcataatc acaatccgcg
    10681 ccgccatggt catcgcttcg gtctgatcgt gggtcacgta gatcatggtg gtgttcagtt
    10741 tctgatgcag cttgctgatc tctgcacgca tttgtacgcg cagcttggca tcaaggttag
    10801 agagcggttc atccattaaa aacacgcccg cttcgcgcac aatcgcccgc ccgagcgcca
    10861 cacgctggcg ttgcccgccg gaaagcgcgc ccggcttgcg tttcaggtac tcacgcaggc
    10921 cgagaatttg cgccgcccag ttcacccgct catcaatcac ctctttggcg attttttgca
    10981 tcttcaggcc aaacgccatg ttgtcgtaaa ccgtcatatg cggatacaac gcgtagttct
    11041 ggaacaccat cgcgatattg cgtgctttgg ctggaacgtc attcattcgt ttgccgtcga
    11101 tcaacagatc gccgccgctg atctcctcaa gcccggcaat catgcgcagg gtggtcgatt
    11161 taccgcagcc cgacgggccg acaaatacga tgaactcttt atcggcaatt tccaggttga
    11221 agtccttcac cacatgcacc tggttatcgt agattttttg aatatgttgt aacgaaagct
    11281 gagccatgtt gattcctttg ctatacgttt tgccagaagg ccgataaccg cgaccaggtc
    11341 agggagtccg ttgagggcaa cagtaattgc gccccggtta agcccgcacc gatccccacc
    11401 gagcgcatac cgctggcgtt aatggcgtca atgcccgcct gcgcatcttc aatgccgata
    11461 catgcctgcg gcggcacgcc cagcccggca caggcagcga gaaagatttc cggatccggt
    11521 ttcgagtttt taagttggga agcatccgcg cagaaggtga aaaactcgcg cagctccagc
    11581 gccgctaaaa tcgtcggcgc attcagggag acggaagcca gccctaccgg aatctgctgt
    11641 gcacggagct ctgccagcaa attgcgaata ccgggcagca cagcattaac cgtcagctcg
    11701 cgcagtgagt ggacgtaaag cagatttttg cgatacgcca gttgcgcccg ctcctgcggg
    11761 ttaaagtcgc cctctttgcc cccgtgttgc agaatgcgcc gcagagactc atcgcggctg
    11821 atccctttta gagattcgtt aaactgcgca tcaatactga tgccaatttc agcggcaatc
    11881 tgctgccacg cctggaaatg cagatgcgcg gtatcggtga ttacaccatc cagatcgaaa
    11941 attacccctt gcagtttcat tcatcctcct gatgcgtggt agcggtccca ttgaagggca
    12001 aaataaaatc acccaaacag aaaacagatt cttcagccac ggatataagc tgaccgttca
    12061 aacgcagtga aacgggcgca gaagttcgaa tcgcaatacg ctgcgcgtcg agagtgacct
    12121 gtaattcgca gccctgccag aacagaggga aagacaactg ttgccactgc tcaagtaacg
    12181 ccggatttag atgcaattca ccgtcacgca cgctcacccc ggcaaaaccc tgaatcgccc
    12241 ccagccagat agcgccagtt gcggcagcat ggataccatc atcacaacta tgcggatcag
    12301 caccaagatc gatttcagtc ccctcgcgcc agaactgata actttgggtc agcaggccac
    12361 agcgtgcagc aacaatgccg tggattgctt tacttaatga cgagtcgtga atagtgcgcg
    12421 gttcataaaa ttgcagattg gcaagacacg atgccgatga gaactgctct ggcagcatgt
    12481 aattgagcat caccacatca gcttgtttga ggatctgcat ctcgttcact tctgcgcgtg
    12541 aataatccag cagaatggtt tgcttccccg ccgccgcttt gtatttcgcc agattaatcg
    12601 ccggcttagt cataaacgaa tcatcctgcg gcaaaacacc gtcgggctga atttctggca
    12661 gccgcagttc tttaaggaac atttcggcac gatgaataaa cgcgtcgtca ctacagccaa
    12721 actggcgggc aatactcagc gcctgctgga cgttgtaata caccatatag ctggtgaagg
    12781 cgttattatt gacatgttcg gtatattcgt ctggcccaat aacatcatga atttccagac
    12841 ggtcgttaac cctcaccgcg cggctaatcc agaactttgc agtttccaga agtagcgcca
    12901 tgccttcatg cgcaatgaaa ctttcatccc ccgtggtctg ccagtattga ataaccgccc
    12961 aggcgatatc ggccaccaga tgatgttccg cctgcgccga ggccactttt tgccgcagcc
    13021 cggtgcgaat gttaatggcg gcaaattccg gcgtctcttc ttcgccgctg cgcgcgcttt
    13081 cccacggaaa tagcgcgccc tgccagccgt tgcgtcgcgc tttctcctgc gcgcctggca
    13141 agttgtgcca gcgataacgc agtaaacttc gggcaaccgt cggatcgcta aacagatgga
    13201 acggcagcaa aaatacttct gtatcccaga aaacgtggcc tttgtagcct tcgccggtta
    13261 agccttttgc cgcaatactg ctgcgctcgc cgtgagccgg cgtcatgatg cgcagatgat
    13321 aaagcgcata atctaacgct tgctgatcgt gcgcatcgcc gccatttacc gtgatacgac
    13381 gtttctgcca ccattgacgc cagttttctg ttgatgctgc aagatgttgg tcgtaactct
    13441 gttgcgcgca catttcaagc tggcgaagcg acacgctgcc ccactcgtct aaagcagctt
    13501 gcctgtcatc ccgccagtcg atccacacca gtttttgcaa cgtcactgtc tcgcctgcat
    13561 gaagctgcgt actggtatgt tgcagtaaac ggcgctcttt ggcggtatag cattgctgca
    13621 catcaccgct cacctggcag caacaactga tggccacatc actgcgccca tcctgggtgg
    13681 tgtagatccc ctgcatcaaa tgctgaccaa acacccgcac ctgggtttcg tcgagatgct
    13741 ggcgaccgtg gttggtttgc gtggcgtcaa tgcctgttga aatcagcact gacgcgtcgg
    13801 cgtccagtgg cataatagtg atttccagcg caatgagcgg cagttggtct gccgaaacaa
    13861 agcgacggct ggcgatggtg taacctgcgc cgttgctggt acgccagaca acgctacggc
    13921 gtaattctcc actggcaaag tcaagttcac gctgccaggc ttcgcgggat aacgagaaaa
    13981 cctcaccatt tatggcaatc tccatcccca agatatcagg cagattcacc agttcgttga
    14041 tttcaccttt tcccgcccga tgatacagcc ccgccagata catccctcgc gtctggcgcg
    14101 tgtaatcttc ttcatggctg gcgcgaagcc caagatagcc gttcccctgc gccatcagcg
    14161 atgcatactt gttcagggta tgctggctga aatggggttc tgataacgtt actggcctgg
    14221 tcataattcc acacgcgtcc ctgtttctgc ggattgatat aacgccgcca ccagttgctg
    14281 gatgatgtac ccctgctcgg cgtcggctat catcacgggc ttgccctgta cgtgattgat
    14341 aaaggcttcc atgctgcgca aatggcggtt gtcgtctgcc atttcccgtt gcatcagcgt
    14401 cattaattca ccgttgttat cggtgtagat atgtgctgga aacagcgtcg caccggcttt
    14461 atcaccacaa aagctgacgt tcataatcaa ctgttcgcgg atgttgagtg caaatgacgt
    14521 ttccagccac agaatgccgc cgttatgaaa ttcaatggtg ccaaacagcg aatcttcgac
    14581 gctgtaagtt gccggatccc actcgccaaa ttgaccacag ctcttttgcg tgccgatctt
    14641 ttgaaaacta tgcgcattca cgcttttcac cgccggaaag cccagcacat acatcgcagc
    14701 atccagcata tgaatgccga tgtcgatcag caggccacca ccctgcagtt ctttattggt
    14761 aaagacaccc cagccgggaa cgccgcagcg acgcagggcg cgggcggtgg taacgtaaat
    14821 ctctcccaaa acgccgttgg tcacctgttc acgcagctgt tgcgtatcga gcgcaaacga
    14881 tggtgaaagt cgtaggccag caccttgccc agtttgcgcg cggtatcgca catttcccac
    14941 gcctgttctg tcgtcatggc gggcggtttt tcgcacatca catggcagcc tgcttccagt
    15001 gccatcaggg tatgttcgta atgaaaacgg ttaggtgagc cgacgctaac cacatcaggt
    15061 ttcaccgcca gcagcatggc ctgcggatcg tcccacacgg atgcattccc gtatttttct
    15121 gccagcgcct gcgcctggga aaggcggctg tcacagacag ccaccagttc cagatcgttg
    15181 cgggtgcagt agtacgaagc atgaacctta tccgccacct ggcccgcgcc tattatcgcg
    15241 acccgcagcg gagagcttgt cattgcactt ttcacaaaga cctcttagca ggtacgcaac
    15301 caggccaacg aatcacggta cgcctgggca ggatcttctg cccggatacg cccttcatac
    15361 accacataac cctgatagtt atcggcgcgc agctgttcaa acagcgcgtg gaaatcgagg
    15421 gtgccactgc ccggctggta acgatggtta tcggcaatat gcacatgacc gagcaagtca
    15481 cggttgtcgt gcagcgcctg cgccaggtta tcttcttcga tgttcatgtg atagaaatcg
    15541 ccgataatct gcacatgttt aagattgttt tcgacgatgt aacgacgagc atcggcgagg
    15601 gtgttgatca tatgatcctg atagcggttt aacggttcga gatacaccac ggttccggta
    15661 cgcgcggcga cctgttccag tacgcgcagg gaatcactca ccagtttgcg gtcgccgtcc
    15721 aggctacgcg gcgaggtcat cggcggtaag cggaaggtaa acatgcccca cgctgccgga
    15781 aaccccccac ttctgccagc gcctcgagaa tgcgttcgat ctgctttaag ccattaagac
    15841 gacgctcttc gataaagtca ccaatccagc cgtcatagcc gccgcaggcg gtggtcactg
    15901 gtaaaccggt ttctttgatc gccgctttga cttcttcgat gttgttgacc agcagtttgc
    15961 cgtcaatctc aaaaccatcg aagcccatct ctttgatata acgaaatctc tcgcggatat
    16021 tttccggaaa gaatgcctga ttctgtgtgc cgattttcat tttttcattc ctttgaatta
    16081 aaacgtaacg cccattttga tgctctgttc cggatgctga tcgacatact gcatgtagct
    16141 ttccgggctg ttggcaaagg tcactaccgg gtcgattaaa tcttcgcaat tgagataacc
    16201 gttcatcagc agttcccagc aggtttcttc aatacgctta cggctccagc gcggataatc
    16261 cgggttcggt tcgctgcacg cgcgggagaa aacaattttg gcgttattga aatgcgcttc
    16321 gcgtccgagg ttaaaacctt cagcaaatgg tttggcaaac gcgacatagg agatggtgcc
    16381 gccgtaggcc aggccgcgca gcgccgattg cagcgcgtcg gcgtaaccgc tggtttcgat
    16441 aatcacatca gccccctgct tgccggtcag cgttttgatc tctttgccta cgtcagtgcc
    16501 aatggggtta aggcagaaat cagcaccgtg gcgacgggca atatcacagc gatgggcgat
    16561 aggatcgacg ccaattacca ccgatgcgcc agcgcgtttc gccagttgga tggcaatttg
    16621 accgatcgcg ccaagcccta ccaccacgac aaaatcccct acgcgtacgt tggcatcgcg
    16681 cacgccgctc atggcaaact gcgccgggtc gtaacagacg gcgtttttcc aggagctgcc
    16741 ttgcggcatt ttgcgcagct tgtagttatt cactgcgtta atgatgaccg tctcggagag
    16801 cgggccgtag ccgcatacgc tgtcgcccac cgcgtagtct gtaacgtcgc tgccgcactc
    16861 gataatgtcg ccaaccacca tgttgccaag ctggaatttg ccaaactcaa tgccgcgcgg
    16921 cgcatctgcc ggacgcgggg tgaacatctg ccactcgccg ttaaagtctt catcgataaa
    16981 cgggctggcg gcgcggaagt cgaccacttc cgttccgtgt ttcggtgcgc cgaaacgcac
    17041 gcggatcttc acttcattgg ctaaaatcgc ccgatcttca tactcaacca gcgcagcaac
    17101 acgcggtgct gtggctacta actttttcat gtaaaactcc ttgtaaagaa actggccgct
    17161 ttagcccttc acgccaccgg cggtcaaacc actcttaata aaacgttccg aaagcgcata
    17221 cataatcacc accggcaatg cggtcaccag cgaggccgcc atcatccgtc cccagatgta
    17281 atcaggcgta ctgaacagcg cgttcaggcc cactggtaag gtgaaattgc tggcgctgga
    17341 gaggaaaatc gacgcaaaca gatagtcgtt ccacgccacc ataaagcagt agacgaacac
    17401 cgatatcagc ccggacatcg ccaggggcac ggtgatacgg aagatgattt gcagacggtt
    17461 gaggccatcc atcatcgccg cttcttcgat ctcatcaggg atggtgtcga agtaactttt
    17521 cagcataaac acggcggtgg gcagggtttg cgtcaccatg gtaatgatca gcgccatctc
    17581 ggtgtcgtaa ataccaagcg cggtgatgat tttgaacaga ggcaccacca gcaaaatgcc
    17641 agagaacata tacaccgtgt aaaagctggc gttgatggtc atccggcctt taaagcgcag
    17701 gcgggaaagc gcgtaagctc cgagaatgcc gagaaatacc gccaccacgg aggaaaccac
    17761 cgacaccacc atgctgttac ggaagtagtc gacaaacggg aaaatcatcg ggttaaaaat
    17821 gtcgacgtaa tgctccagcg tccactgttg cggcagcagc gtaggatgca gcgatatcgc
    17881 ctctttcgcg cttttgaacg aggtcatcag catcacaaaa aacggaaaca gcgtgatgat
    17941 gagaaacagc gccagcccgc agtaaaaacc gatacgactg agcgtgcgtt tatttgttgc
    18001 cattgaggtt cacccgtttc ctggtcagca gaatgacagc gaaaataatg atgaagagca
    18061 ccaccgagat cgccgccgct ttccctaagt cattaaaggc aaaggcggtt ttgtagagat
    18121 agacaccaag aatatcgacc ttggttgtca gcagataaac atcagcgaac atgtagaaca
    18181 tccagatggt gcgcagggtc accaccgtcg ccaggaccgg cataattgcg ggcagcgtga
    18241 cgatgcgaaa ccgctgccag gcattagcgc catccatctc tgccgcttcg tacagcgatt
    18301 tgtcgatggt ctgcaaaatc gccagaaacg agataaaggc atacgggaag tagcgccaga
    18361 tggcgaacag caccaccagc acaaagctac tgcccggatt atcaaaccac agcggtgcct
    18421 gctcatagag atgcagaaga tcgacgccga ggtagttcac aatgccgtag ccgttgttga
    18481 acatgtattt ccaggcaaac accagcgaaa tggacggcgt tacgtaggag aggatcacca
    18541 gcgaacgcgc ggttttgcgc aggcggaact cgcggttgaa aaacatcgcc acggccagcc
    18601 cgagcacggt gctgcccgcc accaccagcg ccgtatacca gaccgtcatc cacagcgagt
    18661 gccagaagcc gggatcggag agaatgcgca catagttact caccccaaca aacgttgact
    18721 cgatgttggg attgagcggc agacgtaaaa aactgatttc gatattcgac accatcggcc
    18781 acgccaccag accgcccagc agtaataagc tgggcgcgag aagcagcagc gcaaacggca
    18841 tatcggaacg acctgaaaaa agcctgttca ttgcggattc ctgtcctggg tttagtgctg
    18901 ttcgatcagt tcatccagct ttttctggct cgcttgcagc gtgccaggga gatcggcttt
    18961 acccacggtg acgttatgca ccatggagct caccacacca gaacccgtca catcgcccat
    19021 gcgggtaaag tttttatccc ctactgcgcc aaagacctga atatttggca gttcaccgat
    19081 tagctgattc ggtagttcac ccagcgcctt aataacgtcg ttgtctttcc aggtggcggt
    19141 agtgacaacc gctttattca ccggcagcgc agcacctggc gacatcatca cccagtcggc
    19201 aatgttgtcc gcctgctcca taaaggtgac aaatttttct gctgcttcgg tcttttgtcc
    19261 ggcggtaatg gtcagcgagg tcaacatgcc gtagaccgca gagtttttct cggttggcac
    19321 cacgaaaccg acgtttttcg ggtcgccttc tttaatcaca gccggaagga tataggtgga
    19381 gtaaatcgcc atcggcgcgg tgccgttcat aaaggcgtct ttcacttcca tgatgtcgtt
    19441 agaacccggc atggtgttgg cagcaaggtc gcggtaatag gtcagcgcct gcatcatctc
    19501 tggtgtatca agggtgattt tgccttcggc gttaaagacg ttggcctagt tggataacgc
    19561 aaactgggag aaggattgtt ccgtcagcac gctttctgcc gtaggcagcg caataccgta
    19621 ttttttattc gccgggtcat tcagtttctg tgcaacgtcc agcagctgtt gccagttttt
    19681 cggctcctcc agccccgctt ttgccagcac atctttgcga taccagatgt cgccaatcca
    19741 ggcgctgaca ggaacaccgg tccatgcgct accatcttcg gtacgcacaa tacgcagtac
    19801 gccatcgtaa aacgcgcctt caccaacgtt gctgatgacc gtggcaaccg ctttgcgatc
    19861 gataagctgc tctttatcca taactttggc gtagtcatgg ctggtttcga tcacttccgg
    19921 cagcgagccg ctacgtgaaa gggtgatgac tttggtgtta tgagcgtctt cttccacggg
    19981 cacttgttta acggtgatac ctgggttttc tttttcaaaa cgggcaatca atttgctgat
    20041 aactgcctga cgctcctgct ccaccgaaga atgcataaat tcgatggata catgggtttt
    20101 attttcttct ttacagcctg aaatcagggc gcaggaaacc agtgctgata acagcacgat
    20161 ttttgattta atcatgagat gttccttttt atttaatcca cataacctgc caggggcgca
    20221 gcgttatttc attacccaat atatcaactt cactaatcag atcccgacca tgcagatcag
    20281 taatatttac atgctgaata tttttactga cattaaacaa tccagtcaga caattaccat
    20341 cagcgttact tctttgaata cgcattacgg atgaattaac cgtatcgatg gtaaaattat
    20401 tatccggatg aaactcgtta tggctgcgac gaagtgtaat taaacgcgac aactcatgat
    20461 ataccgcatg ccttaatgta gcttcatcgt tcagttctcg tgttatctct tcaccgtgat
    20521 attttttacg gttaatcgca cggttatatc cgagtttttc gacacctgcg taatcattac
    20581 gcgagccaag aatactttga atatatatcg cgggtacacc ggggaaactt aacaaaatcg
    20641 catgggcaag gataaacctg gtgcaacgtt
//
LOCUS       DAJTQY010000070        20614 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.070, whole genome shotgun sequence.
ACCESSION   DAJTQY010000070 DAJTQY010000000
VERSION     DAJTQY010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20614
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.070"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            98..397
                     /locus_tag="OO349_002668"
     CDS             98..397
                     /locus_tag="OO349_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5867684.1"
                     /translation="MTELAQLQASTEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLINSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            407..931
                     /gene="ygaP"
                     /locus_tag="OO349_002669"
     CDS             407..931
                     /gene="ygaP"
                     /locus_tag="OO349_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HCR5867685.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(978..1382)
                     /gene="stpA"
                     /locus_tag="OO349_002670"
     CDS             complement(978..1382)
                     /gene="stpA"
                     /locus_tag="OO349_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HCR5867686.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            2050..2499
                     /gene="alaE"
                     /locus_tag="OO349_002671"
     CDS             2050..2499
                     /gene="alaE"
                     /locus_tag="OO349_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HCR5867687.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(2536..2880)
                     /locus_tag="OO349_002672"
     CDS             complement(2536..2880)
                     /locus_tag="OO349_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HCR5867688.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            3032..3262
                     /locus_tag="OO349_002673"
     CDS             3032..3262
                     /locus_tag="OO349_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5867689.1"
                     /translation="MYNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVG"
     gene            3609..3854
                     /gene="nrdH"
                     /locus_tag="OO349_002674"
     CDS             3609..3854
                     /gene="nrdH"
                     /locus_tag="OO349_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HCR5867690.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            3851..4261
                     /gene="nrdI"
                     /locus_tag="OO349_002675"
     CDS             3851..4261
                     /gene="nrdI"
                     /locus_tag="OO349_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HCR5867691.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            4234..6378
                     /gene="nrdE"
                     /locus_tag="OO349_002676"
     CDS             4234..6378
                     /gene="nrdE"
                     /locus_tag="OO349_002676"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HCR5867692.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRASPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFSRTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSCITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            6388..7347
                     /gene="nrdF"
                     /locus_tag="OO349_002677"
     CDS             6388..7347
                     /gene="nrdF"
                     /locus_tag="OO349_002677"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HCR5867693.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNTNKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            7702..8904
                     /gene="proV"
                     /locus_tag="OO349_002678"
     CDS             7702..8904
                     /gene="proV"
                     /locus_tag="OO349_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HCR5867694.1"
                     /translation="MAIKLKIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            8897..9961
                     /gene="proW"
                     /locus_tag="OO349_002679"
     CDS             8897..9961
                     /gene="proW"
                     /locus_tag="OO349_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HCR5867695.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPADGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALITWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            10019..11011
                     /gene="proX"
                     /locus_tag="OO349_002680"
     CDS             10019..11011
                     /gene="proX"
                     /locus_tag="OO349_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HCR5867696.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            11203..12382
                     /locus_tag="OO349_002681"
                     /pseudo
     CDS             11203..12382
                     /locus_tag="OO349_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            12506..13243
                     /gene="ygaZ"
                     /locus_tag="OO349_002682"
     CDS             12506..13243
                     /gene="ygaZ"
                     /locus_tag="OO349_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HCR5867697.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALSVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            13233..13568
                     /gene="ygaH"
                     /locus_tag="OO349_002683"
     CDS             13233..13568
                     /gene="ygaH"
                     /locus_tag="OO349_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HCR5867698.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            13659..14189
                     /gene="emrR"
                     /locus_tag="OO349_002684"
     CDS             13659..14189
                     /gene="emrR"
                     /locus_tag="OO349_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HCR5867699.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRKSDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            14316..15488
                     /gene="emrA"
                     /locus_tag="OO349_002685"
                     /pseudo
     CDS             14316..15488
                     /gene="emrA"
                     /locus_tag="OO349_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
     gene            15505..17043
                     /gene="emrB"
                     /locus_tag="OO349_002686"
     CDS             15505..17043
                     /gene="emrB"
                     /locus_tag="OO349_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HCR5867700.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLNSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDCFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFDAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFCASAWPQFIQGFAVVCFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(17107..17622)
                     /gene="luxS"
                     /locus_tag="OO349_002687"
     CDS             complement(17107..17622)
                     /gene="luxS"
                     /locus_tag="OO349_002687"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HCR5867701.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILEREVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(17772..19328)
                     /gene="gshA"
                     /locus_tag="OO349_002688"
     CDS             complement(17772..19328)
                     /gene="gshA"
                     /locus_tag="OO349_002688"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCR5867702.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            complement(19401..19829)
                     /locus_tag="OO349_002689"
     CDS             complement(19401..19829)
                     /locus_tag="OO349_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5867703.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(19826..20392)
                     /gene="yqaB"
                     /locus_tag="OO349_002690"
     CDS             complement(19826..20392)
                     /gene="yqaB"
                     /locus_tag="OO349_002690"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HCR5867704.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
BASE COUNT         4969 a         5276 c         5151 g         5218 t
ORIGIN      
        1 tcagcttacg cttttactct catttacgct gctgaggctg gtatatgttg catttttagt
       61 ttagagttta cttaatttag aaaatactta aataaatatg actgaactcg cgcaattaca
      121 ggccagtacc gaacaggcag cggccttatt gaaagcaatg agccacccta aacggttgct
      181 gattctgtgc atgcttagcg gttcccccgg caccagcgcg ggagagctga cgcgcattac
      241 cggactgagc gcctctgcga catcacagca tctcgctcgt atgcgggacg aagggcttat
      301 caacagccaa cgggatgccc aacgcattct atattccatt aaaaatgagg cggtaaatgc
      361 cattatcgcc accctgaaaa atgtctattg tccgtaagga gtcactatgg ctttgacaac
      421 catttcgccg catgatgcac aagaattaat cgcacgcggc gcaaagttaa tcgatattcg
      481 tgacgctgat gaatatcttc gtgaacatat tcctgaagca gatctggctc cattatccgt
      541 gctggaacag tcaggtcttc cacctaaatt acgtcgcgag caaattatat tccactgcca
      601 ggcaggtaaa cgcaccagta ataacgccga taaattagca gcgattgccg cccccgcaga
      661 aatattttta ctcgaagatg ggattgatgg ctggaaaaga gctggattgc cagtagcggt
      721 aaataaatct caacccttgc cgttaatgcg ccaggtgcag atcgctgcgg gtggtttaat
      781 attaatcggc gttgtactgg gttataccgt aaatagcggt ttcttcttat taagtggctt
      841 tgttggtgcc gggttactgt ttgcaggaat cagcggtttt tgtggaatgg caaggttgtt
      901 agataagatg ccgtggaacc aacgagcttg agaagcgacg ccggacgcgc cctagcagcg
      961 acatccggcc tcagtaatta gatcaggaaa tcgtcgagag atttaccttc tgccagcgcc
     1021 tgagcaattg gcttcggtgt acggccctga ccggtccagg ttttagtttc accgttaaca
     1081 tcagtgaatt tatatttcgc cggacgcggc tggcgttttt taccagcacg tggtgcagca
     1141 gcagagctat tacccaataa ctcttccggg ttaattccgt cagctttcat cagctccagc
     1201 caggtgctaa ttttttcctg acgctcagcc agttcacgct gctgctgttc ttcttcttca
     1261 cgtctttctt tagtgacaac cctgaatttt tcgagcattt cttcaagaac gtcaatggag
     1321 aattcgcgag ccatcgcacg gagggtgcga atgttattta aactttgtaa cattacggac
     1381 ataataaaga aaacctttta acgccaaaac aaaaaagtat ttcgtcgcgt agtgtaagcg
     1441 atcacttatt atatttccac aaaatttaat ctatttacag tcctgcgcga gattatttca
     1501 gctgttattt tctgaaactc tcagaataat ttcaagatta tatattagcc attttgatga
     1561 gtaatttagg ttattggtga acagagtggc gctctctgct cgctaactcc gcaagcctga
     1621 caaaaccgaa gtgaataaaa atgcaatata acagaataaa agaaaacacc cctcactggt
     1681 gctttctgct gttaatgtac attttctggc atcaaaaatg ctgtaaatga cccggtcacg
     1741 acaaacaccg aattctctac gcttatagcg ttacctcccc cccaaacttt cattagtgaa
     1801 attaaaggca agatcatatc taattgattt aattgaaaat aaatcatatc ggcatgtaaa
     1861 aatccacaat gtacaaaaaa cgagccgtta cggaatattt tatctacaaa aactaactaa
     1921 ataaaaattt ttcactaatt gattagtcat agacagcgat atacgctatg caaaaatgca
     1981 gatggcaatg agatccactg ctttcatctc cattaacatc ccattacgct tttattaagg
     2041 agcattagca tgttctcacc gcagtcacgc ttgcgtcatg cagttgcaga tacgttcgcg
     2101 atggttgttt actgttctgt cgtgaacatg tgtattgaag ttttcctctc cggaatgagc
     2161 ttcgaacagt ctttttattc cagattggta gcgattccgg tgaacatctt aattgcatgg
     2221 ccatacggta tgtaccgcga tctgtttatg cgcgcggcac gcaaagttag cccgtcgggc
     2281 tggataaaaa atctggctga tatcctggct tatgtgacgt tccagtcacc ggtgtatgtg
     2341 gcgatcttgt tagtggtggg cgcagactgg catcagatta tggcggcggt cagttcaaac
     2401 atcgttgttt cgatgttgat gggggcggtt tatggctact tcctcgatta ttgccgccga
     2461 ctgtttaaag tcagccgtta ccagcaggta aaagcctgac tcttcttttc gcgactggca
     2521 tcgccagtcg cgtctttaac tggcttcgcc aaataaacca ttcaaataac gttcaagcgc
     2581 aacacgcgaa ctaaagccat gcgggatacc gtaatgctcg tgtcgctcgc ctgctaaata
     2641 gagcggaaat tgctggtaat gatcgcaagt tttaatatcg gaagcgggtt cagccagtgt
     2701 tgaactgcgt tcttttaatg ttggatgaat taccagagcg gtacgtccca ttcgcgcttc
     2761 gcgattaaca tagacataat tttccccacg gcggtaacca tacgctttct gggttaccac
     2821 atcgacagta aaaccgactt tttcaagtac gcgcgccacc tcgtctggtc gtaaatacat
     2881 atttgatcct cgttattttc aaccgcgggc ctaccttacc tgattgcgca ttcacaacgc
     2941 tttcagaaaa gtccataaag ccgcgatgaa cctcagttaa gaaaattatg gtctacactg
     3001 aaaattacat cgaattctaa tgggggatca tatgtataac cgaccgaacc gcaacgacgt
     3061 tgatgatggc gtgcaggata tccagaatga tgtcaatcaa ttagctgaca gcctcgaatc
     3121 tgtattgaag tcctggggca gcgacgccaa aggggaagct gaagccgcac gcagtaaagc
     3181 tcaggcgttg ctgaaagaaa ccagggcgcg aatgcatggt cgtactcgcg tccagcaagc
     3241 cgcgcgcgat gccgttggct gagcggattc ttttgttcgt gaaagaccct ggtgtagcgt
     3301 gggtacagca gctgcggtag gtatttttat cggcgcactg ttaagcgtgc gcaaatcgta
     3361 gtgcaaaaat gataataaat acgcgtcttt gaccccgaag cctgtcttcg gggtttcttt
     3421 ttgcctggtg aatcacaaaa atccccctcc cccgtcacgc tcatatccag ggtaatttcg
     3481 atcactattt gctatatatt gtgtggttga atcttttttc aactacatct agtatctctg
     3541 tatcaacaga gagacaaccc gacgcgtatc atcgcgccgt atcttcattt taaacggaaa
     3601 tacgaatcat gcgcataact atttatactc gtaacgattg cgttcagtgc cacgccacca
     3661 aacgggcgat ggaaaaccgg ggctttgatt ttgaaatgat taatgtcgat cgcgttcctg
     3721 aagcagcgga agcgttgcgt gctcagggct ttcgtcagtt gccggtagtg attgctggcg
     3781 atcttagctg gtctggtttc cgtccggaca tgattaaccg tctgcatcca gcgccacacg
     3841 cggccagtgc atgagccagc tcgtctactt ctccagcagc tccgaaaaca cgcagcgttt
     3901 tatcgaacgt ttaggtctgc ccgcggtgcg catcccgctc aatgagcggg aacggattca
     3961 ggtagacgag ccttacatcc tgatcgtgcc ctcttacggc ggcggcggta cggctggcgc
     4021 ggtgccacga caggtaattc gctttttaaa cgacgagcac aaccgggcgt tgcttcgcgg
     4081 cgttattgct tctggtaatc gcaactttgg tgaggcgtat ggccgcgccg gagatgtgat
     4141 tgcccggaaa tgcggcgtgc cgtggctgta ccgttttgaa ctcatgggta cgcaaagcga
     4201 tatcgaaaac gttcgtaaag gagtaaccga attttggcaa cgacaaccgc agaatgcctg
     4261 acgcaggaaa cgatggatta ccacgcgctg aatgcgatgc ttaatcttta cgatagcgca
     4321 ggtcgcattc agttcgataa agaccgccag gccgttgacg cctttattgc gacgcatgtg
     4381 cgtccgaaca gtgtgacctt cagtagccag cagcagcgcc tgaactggct ggtcaacgaa
     4441 ggttactatg atgaaagcgt tcttaatcgc tactctcgcg actttgtcat tacgctgttt
     4501 gcccacgcac acaccagcgg ttttcgtttc cagacattcc tcggggcatg gaagttttac
     4561 accagctata cgttgaagac attcgacggt aaacgttatc tggaagattt tgccgatcga
     4621 gtcacgatgg tggcgctgac gctggcacaa ggcgatgaga cgctggcgtt gcaactgacc
     4681 gatgaaatgc tgtcaggacg ctttcagcca gccacgccaa cattcctcaa ctgcggtaag
     4741 cagcagcgcg gcgaactggt ttcctgtttt ttgctgcgta ttgaagacaa tatggagtcg
     4801 attggtcggg cggtaaattc cgcactgcag ctgtcgaaac gcggcggcgg cgtagcattt
     4861 ttgctgtcga atctgcggga agcgggcgcg ccaattaaac gtattgaaaa tcaatcttct
     4921 ggcgtaattc cggtgatgaa aatgttggaa gacgcatttt cctatgccaa ccaactcggc
     4981 gcgcgtcagg gggcaggtgc agtctattta catgctcatc atcccgatat tctgcgtttt
     5041 ctcgacacga aacgggaaaa tgccgacgaa aaaatccgca ttaaaacact gtcgctgggc
     5101 gtggtgatcc cggatatcac tttccatctg gcaaaagaga atgcgcagat ggcgctgttt
     5161 tcgccctatg acgttgagcg agtttatggc aagccgtttg ccgatatcgc cattagcgaa
     5221 cactatgacg aactggttgc cgatgaacgc atccgcaaaa aatacctcaa cgcccgtgat
     5281 ttcttccagc gactggcaga aatccagttt gagtccggct atccctacat catgtatgaa
     5341 gacacagtaa accgtgctag ccctatcgcc gggcgcataa atatgagtaa tctctgctca
     5401 gaaattttgc aggttaacag cgcctcagag tatgacgaga atctcgacta tgcccgcaca
     5461 ggccatgata tttcctgcaa tttaggttcg ttgaatattg cgcacaccat ggattccccc
     5521 gatttttccc gcacggtaga gactgccgtg cgcggtttaa cggcggtgtc agatatgagt
     5581 catatccgca gcgtgccgtc catcgaagcc ggaaatgccg cctcgcacgc catcggactg
     5641 gggcagatga atttacatgg ttatctggcg agggaaggca tcgcttatgg ttcgccggaa
     5701 gccctggatt tcaccaatct ctatttctat accatcacct ggcacgcact gcgtacctcg
     5761 atgttgctgg cgcgcgaacg cggtgaaacc ttcgccgggt tcaaacagtc acgctatgcc
     5821 agtggtgaat atttcagcca atatctgcaa ggtaactggc agccgaaaac ggcgaaagtt
     5881 ggcgaactgt ttgcccgcag ctgtattacg ttacctaccc gtgagatgtg ggcgcaactg
     5941 cgcgacgacg tgatgcgcta cggcatatac aaccagaatc tccaggcggt gccgccaacc
     6001 gggtctatct cttatatcaa ccatgctacg tcgagtattc atccgattgt ggcgaaagta
     6061 gagatacgca aagagggcaa aacaggacgc gtttactacc ctgccccgtt tatgactaac
     6121 gaaaatctgg cgctgtatca ggacgcttac gaaattggcg cagaaaagat catcgacacc
     6181 tacgcggaag cgactcgcca tgtcgatcag gggctgtcgc tgacgctgtt tttccccgat
     6241 accgccacca ctcgcgatat caacaaagcg cagatttacg cctggcgcaa aggtatcaaa
     6301 acgctctatt acatccgcct gcgtcagatg gcgctggaag gcactgaaat tgaaggctgc
     6361 gtctcctgtg cactttaagg aatatctatg aaactctcac gtatcagcgc catcaactgg
     6421 aacaagatat ctgacgataa agatctggag gtgtggaatc gcctgaccag caatttctgg
     6481 ctaccagaaa aggtgccgct gtcgaacgat attcccgcct ggcagacatt aactgtcgta
     6541 gaacaacaac tgacgatgcg cgtttttact ggcctgacgc tgctcgacac gctgcaaaat
     6601 gttatcggcg cgccttcgct gatgcccgat gcgctcacgc ctcatgaaga ggcggtatta
     6661 tcgaatatca gctttatgga agcggttcat gcccgctctt acagttcgat tttctcgacg
     6721 ctgtgccaga ccaaagatgt cgatgccgcc tacgcctgga gtgaagaaaa cgcaccgttg
     6781 cagcgaaaag ctcagattat tcagcaacat tatcgcggtg atgatccgct gaaaaagaaa
     6841 atcgccagtg tgtttcttga atcttttttg ttctattccg gtttctggct gccgatgtat
     6901 ttttccagcc gcggaaagct gaccaatacc gcggacctga tccgtctgat tatccgcgat
     6961 gaagcagtcc acggttacta cataggctat aaatatcaga aaaacatgga aaagatatct
     7021 ctgggacaac gtgaagagtt gaagagtttc gccttcgatt tgttgctgga actctacgac
     7081 aacgagttgc aatacaccga tgagctgtac gccgaaaccc cgtgggctga cgatgtgaaa
     7141 gcgtttctct gttacaacac caataaggct ttgatgaatc tgggctacga accgttattt
     7201 cccgcagaaa tggcggaagt gaatccggca atcctcgccg cgctttcgcc gaatgccgat
     7261 gaaaatcacg atttcttttc cggttcaggc tcctcttatg tgatggggaa agcggttgaa
     7321 acagaagatg aagactggaa tttctgaggg tgttattttc aaaaatatca ctacccgcag
     7381 cagggaaata attcccgcca aatagctttt tatcacgcaa atattctgta gtgatctaca
     7441 ctgatactct gttgcattat tcgcctgaaa ctacaatatt caggcgtttt ttcgctatct
     7501 atgacaaaaa atatcaactt ttctcgattt gctctcagcc cttatatcac ggaaaattcc
     7561 ggcgatttgc tcgcatcaat attcatgcca catttgccct caggggttgc ctcagattct
     7621 cagtatgtta gggtagaaaa aagtgactat tcccattggg taatatatcg acataaacaa
     7681 ataaaggaat cattctattg catggccatt aaattaaaaa ttaaaaatct ttataaaata
     7741 tttggtgagc atccacagcg agcgttcaaa tatatcgaac aaggactttc aaaagaacaa
     7801 attctggaaa aaactgggct atcgcttggc gtaaaagacg ccagtctggc cattgaagaa
     7861 ggcgagatat ttgtcatcat gggattatcc ggctcgggta aatccacaat ggtacgcctt
     7921 ctcaatcgcc tgattgaacc cacccgcggg caagtgctga ttgatggtgt ggatattgcc
     7981 aaaatatccg acgccgaact ccgtgaggtg cgcagaaaaa agattgcgat ggtcttccag
     8041 tcctttgcct taatgccgca tatgaccgtg ctggacaata ctgcgttcgg tatggaattg
     8101 gccggaatta atgccgaaga acgccgggaa aaagcccttg atgcactgcg tcaggtcggg
     8161 ctggaaaatt atgcccacag ctacccggat gaactctctg gcgggatgcg tcaacgtgtg
     8221 ggattagccc gcgcgttagc gattaatccg gatatattat taatggacga agccttctcg
     8281 gcgctcgatc cattaattcg caccgagatg caggatgagc tggtaaaatt acaggcgaaa
     8341 catcagcgca ccattgtctt tatttcccac gatcttgatg aagccatgcg tattggcgac
     8401 cgaattgcca ttatgcaaaa tggtgaagtg gtacaggtcg gcacaccgga tgaaattctc
     8461 aataatccgg cgaatgatta tgtccgtacc ttcttccgtg gcgttgatat tagtcaggtg
     8521 ttcagtgcga aagatattgc ccgccggaca ccgaatggct taattcgtaa aactcctggc
     8581 ttcggcccac gttcggcact gaaattattg caggatgaag atcgcgaata tggctacgtt
     8641 atcgaacgcg gtaataagtt tgtcggcgca gtctccatcg attcgcttaa aaccgcgtta
     8701 acgcagcagc aaggtcttga tgcggcgctg attgatgcgc cgttagcagt cgatgcacaa
     8761 acgcctctta gcgagttgct ctctcatgtc ggacaggcac cctgtgcggt gcccgtggtc
     8821 gacgaggacc aacagtatgt cggcatcatt tcgaaaggaa tgctgctgcg cgctttagat
     8881 cgtgaggggg taaataatgg ctgatcaaaa taatccgtgg gataccacgc cagcggcgga
     8941 cagtgccgcg caatccgcag acgcctgggg tacaccgacg actgcaccgg ctgacggcgg
     9001 tggtgctgac tggctgacca gtacgcctgc gccaaacgtc gagcatttta atattctcga
     9061 tccgttccat aaaacgctga tcccgctcga cagttgggtc actgaaggga tcgactgggt
     9121 tgttacccat ttccgtcccg tcttccaggg cgtgcgcgtt ccggttgatt atatcctcaa
     9181 cggtttccag caattgctgc tgggtatgcc agcgccagtg gcgattatcg ttttcgctct
     9241 catcacctgg cagatttccg gggtcggaat gggcgtggcg acgctggtct cgctgattgc
     9301 catcggcgca atcggtgcct ggtcgcaggc aatggtgact ctggcgctgg tgttaactgc
     9361 cctgctgttc tgtatcgtca tcggtttgcc gttggggata tggctggcga gaagtccgcg
     9421 agcggcgaaa attattcgtc cactgcttga tgccatgcag accacgccag cgtttgttta
     9481 tctggtgcca atcgtcatgc tatttggtat cggtaacgtg ccgggcgtgg tggtgacgat
     9541 catctttgcg ctgccgccga ttatccgtct gaccattctg gggattaacc aggttccggc
     9601 ggatctgatt gaagcctcgc gctcattcgg tgccagcccg cgccagatgc tgttcaaagt
     9661 tcagttaccg ctggcgatgc cgaccattat ggcgggcgtt aaccagacgc tgatgctggc
     9721 cctttctatg gtggtcattg cctcgatgat tgccgtcggc gggctgggtc agatggtact
     9781 tcgcggtatc ggtcgtctgg atatggggct tgccaccgtt ggcggcgtcg ggattgtgat
     9841 cctcgccatt atcctcgatc gcctgacgca ggccgttggg cgcgactcac gcagtcgcgg
     9901 caaccgtcgc tggtacacca ctggccctgt tggtctgctg acccgcccat tcattaagta
     9961 actctgcact tgcccggtgg cgccgggcat tatcaccctg ccaaaaaaag gaataacaat
    10021 gcgacatagc gtactttttg cgacagcgtt tgccacgctt atctctacac aaacttttgc
    10081 tgccgatctg ccgggcaaag gcattactgt taatccagtt cagagcacca tcactgaaga
    10141 aaccttccag acgctgctgg tcagtcgtgc gctggagaaa ttaggttata ccgtcaacaa
    10201 acccagcgaa gtggattaca acgttggcta cacctcgctt gcttccggcg atgcaacctt
    10261 caccgccgtg aactggacgc cactccatga caacatgtac gaagctgccg gtggcgataa
    10321 gaaattttat cgtgaagggg tatttgttaa cggcgcagca cagggttacc tgatcgataa
    10381 gaaaaccgcc gaccaataca aaatcaccaa catcgcacaa ctgaaagatc cgaagatcgc
    10441 caaactgttc gataccaacg gcgacggtaa agcggattta accggttgta accctggctg
    10501 gggctgcgaa ggtgcgatca accaccagct tgccgcgtat gaactgacca acaccgtgac
    10561 tcataatcag gggaactacg cagcgatgat ggccgacacc atcagtcgct acaaagaggg
    10621 caaaccggtg ttttattaca cctggacgcc gtactgggtg agtaatgagc tgaagccagg
    10681 gaaagatgtg gtctggttgc aggtgccgtt ctccgcactg ccgggcgata aaaatgccga
    10741 taccaaactg ccgaatggcg cgaattacgg cttcccggtc agcaccatgc atatcgttgc
    10801 caacaaagcc tggaccgaga aaaatccggc ggcagccaaa ctgtttgcca ttatgcagtt
    10861 gccagtggcc gatattaacg cccagaacgc cattatgcat gacggcaaag cctcagaagg
    10921 cgatattcag ggccacgttg atggctggat caaagcccac cagcagcagt tcgatggctg
    10981 ggtgaatgag gcgctggcag cgcagaagta atttttattc gggcggataa ggcgttttcg
    11041 cctcatccgc cgctctgtac acaatgcctg atgcgacgct gacgcgtcct atcatgccta
    11101 caaaccacat cgcacatttc aacaagctat ctttcatccc atattcatca acatccgcta
    11161 ttattgattt ccagcttaat catcacctga tgaacaaaaa taatgactaa acctaatcat
    11221 gagcttagcc cggcgctgat cgtgctgatg tctatcgcca ccggtctggc ggtagccagt
    11281 aactattacg cccagccatt gctcgacacc atcgcgcgta acttttccct ttccgccagt
    11341 tcggcaggct ttattgttac cgccgcgcag ttgggctatg ccgcaggtct actgtttctt
    11401 gttcccctcg gtgatatgtt tgaacgccgc cgcctgattg tctcgatgac cttactggca
    11461 gcgggcggta tgttaatcac tgccagcagt cagtcactgg cgatgatgat cctcggtacg
    11521 gcattaaccg gtttattctc agtcgtggca caaattctgg ttccgctggc agcgacgctg
    11581 gcttcaccgg ataaacgcgg caaagtggtt ggcaccatta tgagcggtct gctgttgggg
    11641 atattgctgg cacgaaccgt tgccgggtta ctggcaaacc tcggcggctg gcgcaccgtc
    11701 ttttgggttg cttcggtgtt aatggcactg atggcgctgg cattatggcg tggtctgcca
    11761 caaatgaaat cagaaaccca cctcaactac ccacagttgt tgggttccgt tttcagcatg
    11821 ttcatcagcg ataagatcct gcgcacccgc gcgttgctgg gctgcatgac ctttgccaac
    11881 ttcagcattc tctggacctc aatggccttt ttgcttgccg ctccaccttt taactacagc
    11941 gatggtgaca ttggtctgtt tgggcttgcg ggagctgccg gagcgttggg cgctcgtccg
    12001 gcgggcggtt ttgccgacaa gggcaaatcg caccacaact ttcggtctgc ttctgctgtt
    12061 actttcatgg ctggcaatct ggtttggtca cacttccgta ctggcgttga ttatcggcat
    12121 tctggtgctg gacctcaccg tacagggcgt gcatatcact aaccagacgg taatttatcg
    12181 aatacatcct gatgcgcgta atcgcctgac cgcaggttac atgaccagct actttattgg
    12241 cggtgccgcc ggttcgctaa tttcagcctc agcctggcaa catggcggtt gggctggcgt
    12301 ttgtctggct ggcgcgacga ttgccctggt taacttactg gtctggtggc gaggttttca
    12361 tcgtcaggaa gccgcaaatt aagcaatcag caaccgtttt cgtggcttta cacttataag
    12421 ggtgttaaga agcccatcag tctgataagg ttaagatatt cattcagtct atttataata
    12481 ttaacaatcg ttaagcgtac actctatgga aagccctact ccacagcctg ctcctggttc
    12541 ggcgaccttc atggaaggat gcaaagacag tttaccgatt gttattagtt atattccggt
    12601 ggcctttgcg ttcggtctga atgcgacccg tctgggattc tctcctctcg aaagcgtttt
    12661 tttctcctgc atcatttatg caggcgcgag ccagttcgtc attaccgcga tgctggcagc
    12721 cgggagtagt ttgtgggttg ctgcactgag cgtcatggca atggatgttc gccatgtttt
    12781 gtatggcccg tcactgcgta gccgtattat tcagcgtctg caaaaatcga aaaccgccct
    12841 atgggcgttt ggcctgacgg atgaggtttt tgccgccgcc accgcaaaac tggtacgcaa
    12901 taatcgccgc tggagcgaga actggatgat cggcattgcc ttcagttcat ggtcatcgtg
    12961 ggtatttggt acggtaatag gggcattctc cggcagcggc ttgctgcaag gttatcccgc
    13021 cgttgaagct gcattaggtt ttatgcttcc ggcactcttt atgagtttcc tgctcgcctc
    13081 tttccagcgc aaacaatctc tttgcgttac cgcagcttta gttggtgccc ttgcaggcgt
    13141 aacgctattt tctattcccg tcgccattct ggcaggcatt gtctgtggct gcctcactgc
    13201 gttaatccag gcattctggc aaggagcgcc cgatgagcta tgaggttctg ctgcttgggt
    13261 tactggttgg cgcggcgaat tattgcttcc gctatttgcc gctgcgcctg cgtgtgggta
    13321 atgcccgccc aaccaaacgt ggcgcggtag gtattttgct cgacaccatt ggcatcgcct
    13381 cgatatgcgc tctgctggtt gtctctaccg caccagaagt gatgcacgat acacgccgtt
    13441 tcgtgcccac gctggtcggc ttcgcggtac tgggtgccag tttctataaa acacgcagca
    13501 ttatcatccc aacactgctt agtgcgctgg cctatgggct cgcctggaaa gtgatggcga
    13561 ttatataacc cacaagaatc atttttctaa aacaatacat ttactttatt tgtcactgtc
    13621 gttactatat cggctgaaat taatgaggtc atacccaaat ggatagttcg tttacgccca
    13681 ttgaacaaat gctaaaattt cgcgccagcc gccacgaaga ttttccttat caggagatcc
    13741 ttctgactcg tctttgcatg cacatgcaaa gcaagctgct ggagaaccgc aataaaatgc
    13801 tgaaggctca ggggattaac gagacgttgt ttatggcgtt gattacgctg gagtctcagg
    13861 aaaaccacag tattcagcct tctgaattaa gttgtgctct tggatcatcc cgtaccaacg
    13921 cgacgcgtat tgccgatgaa ctggaaaaac gcggttggat cgaacgtcgt aaaagcgata
    13981 acgatcgccg ctgcctgcat ctgcaattaa cggaaaaagg tcacgagttt ttgcgcgagg
    14041 ttttaccacc gcagcataac tgcctgcatc aactctggtc cgcgctcagc acaacagaaa
    14101 aagatcagct cgagcaaatc acccgcaaat tgctctcccg tctcgaccag atggaacaag
    14161 acggtgtggt tctcgaagcg atgagctaac gcgtcatctc gctcaaaaat ccagatttat
    14221 aaaagaaaaa atgaccggcc agcatcgcaa catgctggcc tttttggcaa gcaggtcggc
    14281 tcagccgatg agttaagaag atcgtggaga acaatatgag cgcaaatgcg gagactcaaa
    14341 ccccgcagca accggtaaag aagagcggca aacgtaagcg tctgctcctc cttctcacct
    14401 tgctctttat aattattgcc gtagcgatag ggatttattg gtttttggta ctgcgtcatt
    14461 tcgaagaaac cgatgacgca tacgtggcag ggaatcagat tcaaattatg tctcaggtgt
    14521 ctggcagcgt gacgaaagtc tgggccgata acaccgattt tgtaaaagaa ggcgatgttc
    14581 tggtcactct cgacccgaca gatgctcgcc aggcgtttga aaaagccaaa actgcactgg
    14641 cttccagcgt tcgccaaacc caccagctga tgattaacag caaacagttg caggcgaata
    14701 ttgaggtgca aaaaatcgcc ctcgcgaaag cacaaagcga ctacaaccgc cgtgtgccgc
    14761 tgggcaatgc caacctgatt ggtcgcgaag agctgcaaca cgcccgcgac gccgtcacca
    14821 gtgcccaggc gcaactggac gtcgctattc aacaatacaa tgccaatcag gcgatgattc
    14881 tggggactaa actggaagat cagcctgccg tgcaacaggc tgccaccgaa gtacgtaacg
    14941 cctagctggc gctggagcgt acccgtattg tcagtccgat gaccggttat gtttcccgtc
    15001 gtgccgtgca accgggtgcg caaattagcc caacgacgcc gctgatggcg gtcgttccag
    15061 ccaccaatat gtgggtggat gccaacttta aagagacgca gattgccaat atgcgcatcg
    15121 gtcagccggt caccatcacc accgatattt acggcgatga tgtgaaatac accggtaaag
    15181 tggttggtct ggatatgggc acaggtagcg cgttctcact gcttccggcg caaaatgcga
    15241 ccggtaactg gatcaaagtc gttcagcgtc tacctgtgcg tatcgaactg gaccagaaac
    15301 agctggagca atacccgctg cgcattggtt tgtccacgct ggtgagcgtc aataccacta
    15361 accgtgacgg tcaggtactg gcaaataaag tacgttcaac accggtcgcg gtaagtaccg
    15421 cgcgtgaaat cagcctggca cctgtcaata aactgatcga cgatatcgta aaagctaacg
    15481 ctggctaatc cagaggtgcg tgtgatgcaa cagcaaaaac cgctggaagg cgcgcaactg
    15541 gtcattatga cgattgcgct gtcactggcg acattcatgc aggtgctgaa ctccaccatt
    15601 gctaacgtgg cgatccccac tatcgccggg aatctgggct catcgctcag ccaaggaacg
    15661 tgggtaatca cttctttcgg ggtggcgaat gccatctcga tcccgcttac cggctggctg
    15721 gcaaagcgcg tcggggaagt gaaactgttc ctttggtcca ccatcgcctt tgctattgcg
    15781 tcttgggcgt gtggtgtctc cagcagcctg aatatgctga tcttcttccg cgtgattcag
    15841 gggattgtcg ccgggccgtt gatcccgctt tcgcaaagtc tattgctgaa taactacccg
    15901 ccagccaaac gctcgatcgc gctggcgttg tggtcgatga cggtgattgt cgcgccaatt
    15961 tgcggcccga tcctcggcgg ttatatcagc gataattacc actggggctg gatattcttc
    16021 atcaacgtgc cgattggcgt ggcggtggtg ttgatgacac tgcaaaccct gcgcggacgt
    16081 gaaacccgca ccgaacggcg gcggattgat gccgtggggc tggcactgct ggttattggt
    16141 atcggcagcc tgcagattat gctcgaccgc ggtaaagagc tggactgttt ttcatcacag
    16201 gaaattatca ttcttaccgt ggtggcggtg gtggctatct gcttcctgat tgtctgggag
    16261 ctgaccgacg ataacccgat agtcgatctg tcgttgttta agtcgcgcaa cttcaccatc
    16321 ggctgcttgt gtatcagcct cgcgtatatg ctctacttcg acgctattgt tctgctgccg
    16381 cagttgttgc aggaggtcta cggttacacg gcgacctggg caggtttggc ctctgcgccg
    16441 gtagggatta ttccggtgat tctgtcgccg attatcggcc gcttcgcgca taaactggat
    16501 atgcggcggc tggtaacctt cagctttatt atgtatgccg tctgcttcta ctggcgtgcc
    16561 tatacctttg aaccaggtat ggatttttgc gcgtcggcct ggccgcagtt tatccagggg
    16621 tttgcggtgg tctgcttctt tatgccgctg accaccatta cgctgtctgg tttgccaccg
    16681 gaacgactgg cggcggcatc gagcctctct aactttacgc gaacgctggc ggggtctatc
    16741 ggcacgtcga taaccacgac catgtggacc aaccgcgagt cgatgcacca tgcgcagttg
    16801 actgagtcgg taaacccgtt caacccgaat gcccaggcga tgtacagtca actggaaggg
    16861 cttgggatga cgcaacagca ggcatcaggc tggattgccc agcagatcac caatcagggg
    16921 ctgattattt ccgccaatga gatcttctgg atgtcagccg ggatattcct cgtactgctg
    16981 gggctggtgt ggtttgctaa accgccattt ggcgcaggtg gcggcggagg cggtgcgcac
    17041 taagtacaac taagccagtt catttgaact ggcttttttc aattaattgt gaagatagtt
    17101 tactgactag atgtgcagtt cctgcaactt ctctttcggc agtgccagtt cttcgttgct
    17161 gttgatgcgt acctcacgtt ccagaatgct acgcgcaata tcctgcgctt cctgcaacga
    17221 gtgcatctgg taagtgccac actggtagac gttcagttcc gggatctgat tctgatcctg
    17281 cactttcagc acgtcttcca ttgccgcttt ccaggcatca gcaacacgct gctcatctgg
    17341 cgtaccaatc agactcatat aaaaaccggt gcggcagccc attggcgaga tatcgataat
    17401 ctctacacca ttaccgttaa gatggttacg cataaaacca gcaaacaggt gctccagggt
    17461 atggatccct ctttctggca tcacttcttt gttcggcacg cagaagcgca gatcgaacac
    17521 ggtgattgcg tcgccatgcg gggtgttcat tgttttcgcc acccgaactg caggcgcttc
    17581 catccgggta tgatcgactg tgaagctatc taacaacggc atttagccac ctccggtaat
    17641 ttttttaaaa attttctgaa ctctttcttc ccaggcgagt ctgagtatat gaaagacgcg
    17701 catttgttat catcatccct gaattcagag atgaaatttt ggccactccc gagtggcctt
    17761 tttcttttct gtcaggcgtg tttttccagc cacaccgcaa acggttcggt atcagcggtt
    17821 tccatttcct gctgacggcg ttcagatgcc tcgcgctcgg ctacaaaatc ctcttcgcgc
    17881 agaatttcca gcggctcttc acgcagcaga ttacggtagg cttctgcaaa cgctttgcct
    17941 gttccgccaa taccagtatc aatcatagac cttaagatac gggcagagaa agtcagatcg
    18001 ggattatcga agcaggcaac cagttcatca cacactttct gatacgcttc gccgccgtta
    18061 atgctatcca gcgtttgcgc gacgcgtttc agatcgcgga acagatcttt acccacctgc
    18121 agtaacggga actgcgcggt ttcgcagccg atacccagcg tcaggcccgg tttgcgacct
    18181 tcaaggatca cccggttcca gttaacgcgc gtacaggcaa gttcgctact gctcatttcc
    18241 ggtgcatcag ccagcgcaca ccagaccata aacaggtcga ggaatcgcac ctgctgttca
    18301 tctacaccaa tcggcgagaa cgggttgatg tccagcgaac gcacttcaat atattcaatg
    18361 ccgccacgta acagcgcatc agaaggtgac tcgccgctgc gggtaacgcg ttttggacga
    18421 atcggcgcat acagttcgtt ttcaatctgc agcacgttgc tattgatttg cagcctctta
    18481 ccgtctttct caataccaat cttcgcgtac tcttccgatg gcgttttgat tgcctgttta
    18541 aggcccgcta cgtactcgta aagatcgttg aaggtaatac caagattgct ttgcgattta
    18601 ttggtatagc cgagatcgct caaacgaaga gaggtcgcat acggcaggta atacataccg
    18661 cactcggttt tctcaaacgg cagcgacgtt ggttttcctt gcaggaaaga agaacaaatc
    18721 gccggagatg caccaaacag ataaggaatg acccaaccga aacgatagta attgcggata
    18781 acgcggaaat agcccgcaga aattttctct ttggcatcag cgcccgagat atcaccgcac
    18841 ttcgcttgcc agaatgccat tggcaaagag aaattgtagt gcacgccgga aatggtttgc
    18901 atcagcgcgc cgtagcgatt tttcagccct tcacgataca gcgttttaaa gcgtccggtg
    18961 ttagaagtgc cgtactgtgc cagttcgatg tcctgacctt ctgcgatgta gcatggcata
    19021 cttaacggcc acatccgctc atcgcccata ttgcgcgccg tataacgatg cagatcgcgc
    19081 ataaaggtca gcatatgttc aatatcacca tccactggtg taatgaattc cagcaatgct
    19141 tccgcaaaat cggtagtaat ccatttgtgc gtcagtgcgg aacctaatgc ttcaggatga
    19201 cctgttgttg ccagtgtgcc atcagcatta acacgcaaag tttcgcgctc cagcccacgc
    19261 tgtatcccct ttaacgcctg aggatgtttt tccagccagg ccagcgcctg tgatacgtcc
    19321 gggatcaaat tgacctcccg cctgtcaaaa tcgttttaat tagcataact gtaatggtga
    19381 ccatatgtgc aggcctacaa ttagtgccac cacatcatgc cctgaacggt cgctgctgca
    19441 actgcaacat agcgtaacgc tttaccaagg cacaaaaaaa agattactgg cccccaggag
    19501 atgcgcatcc atcccgctaa cagacacagt aaatcgccaa ccacgggcat ccagcttaat
    19561 aatagcgtga ctgcaccata gcgtttcagc cagccggtag ctttctcttg ccagcgcgat
    19621 gttttacgca atggaaagaa acgcccaagg ataacgttag ttaaccctcc aaggctatta
    19681 cccattgttg ctgttaaaac taaaacccag ggatgactga tcccggaaag caacattgcc
    19741 accagcacga cttcggagtt gccgggtaat agtgtagcgc tgagaaaact actggcaaac
    19801 aacgagaaaa gcgataacgc ttcactcaca gcaagcgaac atccacagcg tccatgcctg
    19861 ctgcacgggc cgcctgaata ccgaaatcgg catcttcaaa gaccacacac tgcgtcggtt
    19921 gcacgcccat acgctgcgcg cacaacaaaa atgtgtctgg cgcgggttta tggtgtttga
    19981 cgtgatcagc agcgacgacg gcgtcaaaat aacggcgtaa tcccaggtgc gccagcaatg
    20041 cctcagcgat ggcgctttca ctccccgttc ctacagccat tgggcgacga ccatgccaac
    20101 ttttcaccac ttcaacaaga ggaagcggct cgacactatc cagcagcata cttcttactg
    20161 cttctgtttt ttcacgcgct aacgcatgcg ggtcgagatc ggcctgattc agctcaataa
    20221 ttgcctgagc aatacgccag gtgggcgatc cattaagcgc aatcatcgcc tgaatatcgt
    20281 actgaagacc gtagtgccct aatacttcgc gccacgcttt acggtgcgta ggctccgtat
    20341 ccaggattgt gccatccata tcaaaaatta aacctgcata acgctcgtac atggtcttct
    20401 cgcaagtcga aaaatatgac gctactttag cgtaattgct tgattttgtc gctgatggcg
    20461 aaggagaaga aaattcagga aatggataaa gtagttattt agaaatgaga tatttttgaa
    20521 ggaaattttt tggagaatat ggtgcatccg ggaggattcg aacctccgac cgctcggttc
    20581 gtagccgagt actctatcca gctgagctac ggat
//
LOCUS       DAJTQY010000071        20569 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.071, whole genome shotgun sequence.
ACCESSION   DAJTQY010000071 DAJTQY010000000
VERSION     DAJTQY010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20569
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.071"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            409..1239
                     /locus_tag="OO349_002691"
     CDS             409..1239
                     /locus_tag="OO349_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HCR5867705.1"
                     /translation="MSMPLSNAANNQFNTNNHFLHHPKVDSELTRRYEYARLDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLNIGKQSWPFNYVTDTCREFNGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIEMGN
                     DPIIGIEAYPGTAEIHSKMGYNVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1380..2153)
                     /gene="uxuR"
                     /locus_tag="OO349_002692"
     CDS             complement(1380..2153)
                     /gene="uxuR"
                     /locus_tag="OO349_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HCR5867706.1"
                     /translation="MKSVTSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(2368..3828)
                     /locus_tag="OO349_002693"
     CDS             complement(2368..3828)
                     /locus_tag="OO349_002693"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HCR5867707.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRMLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GTQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMGYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(3909..5093)
                     /gene="uxuA"
                     /locus_tag="OO349_002694"
     CDS             complement(3909..5093)
                     /gene="uxuA"
                     /locus_tag="OO349_002694"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HCR5867708.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            5433..6776
                     /gene="gntP"
                     /locus_tag="OO349_002695"
     CDS             5433..6776
                     /gene="gntP"
                     /locus_tag="OO349_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HCR5867709.1"
                     /translation="MHVLNILWVVFGIGLMLILNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(7027..7929)
                     /gene="fimH"
                     /locus_tag="OO349_002696"
     CDS             complement(7027..7929)
                     /gene="fimH"
                     /locus_tag="OO349_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HCR5867710.1"
                     /translation="MKRAITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPVVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(7949..8452)
                     /gene="fimG"
                     /locus_tag="OO349_002697"
     CDS             complement(7949..8452)
                     /gene="fimG"
                     /locus_tag="OO349_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HCR5867711.1"
                     /translation="MKWCKRGYVLAAMLALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(8465..8995)
                     /gene="fimF"
                     /locus_tag="OO349_002698"
     CDS             complement(8465..8995)
                     /gene="fimF"
                     /locus_tag="OO349_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HCR5867712.1"
                     /translation="MRNKPFYLLCAFLWLAVSRVLAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAAAGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(9005..11641)
                     /gene="fimD"
                     /locus_tag="OO349_002699"
     CDS             complement(9005..11641)
                     /gene="fimD"
                     /locus_tag="OO349_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HCR5867713.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLVVACAFAAQAPLSS
                     ADLYFNLRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVAGMNLLADDACVPLTTMVQDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWRLRDNTTWSYNSSDSSSGSKNKWQHIITWIERDIIPLRSRLTLGDGYTQGDIFDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIYGIARGTAQVTIKQNGYGIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMEALGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCV
                     ANYQLPPESQQQLLTQLSAECR"
     gene            complement(11708..12433)
                     /gene="fimC"
                     /locus_tag="OO349_002700"
                     /pseudo
     CDS             complement(11708..12433)
                     /gene="fimC"
                     /locus_tag="OO349_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709919.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
     gene            complement(12470..13009)
                     /locus_tag="OO349_002701"
     CDS             complement(12470..13009)
                     /locus_tag="OO349_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5867714.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAAGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIASNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(13074..13622)
                     /gene="fimA"
                     /locus_tag="OO349_002702"
     CDS             complement(13074..13622)
                     /gene="fimA"
                     /locus_tag="OO349_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HCR5867715.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTIHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAID
                     ATRTDVLALQSSAAGSATNVGVQILDRTGNALTLDGATFSAQTTLNNGTNTIPFQARY
                     YAIGEATPGAANADATFKVQYQ"
     gene            complement(14102..14698)
                     /gene="fimE"
                     /locus_tag="OO349_002703"
     CDS             complement(14102..14698)
                     /gene="fimE"
                     /locus_tag="OO349_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HCR5867716.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GNRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
     gene            complement(15176..15778)
                     /gene="fimB"
                     /locus_tag="OO349_002704"
                     /pseudo
     CDS             complement(15176..15778)
                     /gene="fimB"
                     /locus_tag="OO349_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
     gene            17232..17948
                     /gene="nanC"
                     /locus_tag="OO349_002705"
     CDS             17232..17948
                     /gene="nanC"
                     /locus_tag="OO349_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HCR5867717.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            17968..19074
                     /gene="nanM"
                     /locus_tag="OO349_002706"
     CDS             17968..19074
                     /gene="nanM"
                     /locus_tag="OO349_002706"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HCR5867718.1"
                     /translation="MNKTIMALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPREQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            19139..20119
                     /locus_tag="OO349_002707"
     CDS             19139..20119
                     /locus_tag="OO349_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HCR5867719.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHSRIKQL
                     ARFAHTHPGGPTCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDRFVEAILQFWRER"
BASE COUNT         5478 a         4908 c         4672 g         5511 t
ORIGIN      
        1 acgtagatga aaatacttgt caccgctgct acttaattcc ggtgggaaat tatttatttc
       61 cgcataatca gctatgtatt atttgaaaca attagtttgt aaataatata aattgataac
      121 atatcaaata catcaaaaat accattcaca ggcattatta atatagacct caatatagca
      181 atatcaaatt ccaattagta ataagatatg cttttaaatt gcatgcaaat aattttaaat
      241 gagcgttttt tgatacttta gaatgtttcc taccctttaa tatcactgtg tagagttgat
      301 atcactacaa cagaaacatg catgtcattt gtttacttct ctttttacta cagaaaaatt
      361 tatctatttt tctaaacatc agttaaatac tcataaaagg taactattat gtctatgcca
      421 ttaagcaatg ccgctaataa ccaattcaat acaaataatc acttccttca tcatccaaaa
      481 gtcgacagtg agttaacacg tcgatatgaa tatgcacgcc tggatacgga aaatatctat
      541 cttctgcctc ttgctcgtgg caataaccat aattacgatg gaaaaagcgt tgtcgaaatt
      601 agaaaactta atattggcaa acagtcctgg ccatttaatt atgtgacaga tacatgcaga
      661 gaattcaatg gtattacaac aacaggacgt atgctctatc gaaacttgaa aatcacctca
      721 gctttagacg aaatatatgg tggtatatgt aaaaaggcgc atgctgctac agagttagca
      781 gaaggtttac ggctaaattt atttatgaag agccctttcg atcctgttga ggactataca
      841 gttcatgaaa taacattagg ccctggttgc aatgttcctg gttatgccgg aaccacgata
      901 ggatatattt caacccttcc agcttctcag gcaaaaagat ggaccaatga gcagccaaga
      961 attgatatct atattgatca gataataact gtctcagggg tagcaaattc ttccggattc
     1021 gcccttgccg cattacttaa tgctaatatt gagatgggaa atgacccaat cattggtata
     1081 gaagcctatc ctggtaccgc ggagatccat tcaaaaatgg gatataacgt tatccccggt
     1141 gatgaagatg cgccactcaa acgcatgact ctgcaaccgt catctcttcc agaactgttt
     1201 gaattaaaaa atggtgaatg gaattatatt ggtaaatagc cactgagaag aatgagtact
     1261 aatattcagg ttatacagat atttaaacaa aaccctgcaa tttacaaagt aatactcatt
     1321 gcgacttctt aatttaagaa agcgcaagga acgtttaccc ttgcgcttat tataataagt
     1381 caggcgtcga ctttatccag cggccatgaa tcaaagagat agccgtcaaa ataaatatcg
     1441 tcaacgttcg agaattccag cagacgttgc ttaacgtttt ccagatgctg ccacattgcc
     1501 agcttcgccg ctcgggcatc ttttttgatt aacgcggcga ggatctgttt gtgatcgccc
     1561 aaccactctt tgcgatacag gctgtcatcc agatggctgt gcaactggat ccacattgga
     1621 ttgttttccc gccactgcca ggactgacgg aataactcca ccagcatgct gttatgcgtt
     1681 gcttcagcaa tggcgagatg gaactgcatg tcaccgcttt cgctgctacc cggcgcgctg
     1741 gaagccagtt cacgctcttc cagttgcaat gcctgacgca ttttgacgat atcttcgcgg
     1801 gtagcctgca gtgcggcaaa ctcggcgatg ttactttcca gtaactgacg cgcctgtaac
     1861 agctcaaaag gacccgcatc attgcagacg ttggcatccg gactgtctgt gttgtggctg
     1921 cctgagctat caagcacata gataccggca ccccggcgca cttccaccag ccctttgatc
     1981 tccagcatga tcagcgcttc acgtaccacc gtccgcgtga catcaagcat ttctgcaatt
     2041 tcacgctccg gcggcagccg ttcgccagga ttgtacggcg tctttatgat cagatcgcgg
     2101 atcatcgccc cgacttcctg gtaaggtctt tgcgcagagg tgacagattt catggtgttg
     2161 tgtccgttag gtgcggttaa tcaaattttt gccggatgcg gcgtgaacgc cttatccggc
     2221 ctacaacggg caattttacg gcccccgtta atctcaaact ataccattag cactggaagt
     2281 tggcacgtac aggtgggcaa aaaaacgaaa actggtcaac caaatatcag tttgattgac
     2341 cagtaagtct gtagggtaat cagttagtta cagcgcagcc acacactcgc gcgcaccgcg
     2401 ttcgcacagc tgctgatatg ccgccgtcac cgcgccaaca aagtcggcat tctgcggcag
     2461 atcatcggca aaaataccgc tcaggcccag cagcgctttc acgcggtctg cgccctgata
     2521 ctgcgcgtta atcttctgga actcggccag catcgggtcg accacgtcaa tggcattacc
     2581 ctgctcatcc acgccctggg tgtaacccat ccagccagcc acgcccagcg ccaggtgacg
     2641 ccagctgccg ccgttttgca ggtgcagacg caccgggtcc agtagacgct gcggtaactt
     2701 ctggctgccg tccatcgcaa tctgccaggt acggtgacgc agagacgggt tgctgaaacg
     2761 ctcgatcagc agcgtcgcat aggcgttcag gtccgtacct tccggcatcg acagcgttgg
     2821 cgcttgttcc tgcatcatca gggcaaaggc cgctttgcga taatccgggt tagtcatggt
     2881 gtcggcaatg gtttcatagc cgccgaggta accgaggtac gccaggaaag agtggctgcc
     2941 gttcagcata cgcagcttca tcatttcgaa cggcacaacg tctgcaacga actgtgtgcc
     3001 cactttatcc cagtccgggc gaccattaac gaagttatct tcaatcaccc actgacggaa
     3061 cggttcgcag gcaatggcgc acgggtcgta aacacccagc tggtcagcaa tttcctgtaa
     3121 ggtttctggc gtcgccgccg gaacgatgcg gtcaaccatg gtgcacggga aggtgacgtt
     3181 ctcttcaatc catgccgcta gctgcgggtc acgcgcctga gccagcccca gtaccgcgac
     3241 cttcgccaca tggccgtttt cacgcacgtt atcgcaggac atcaccgtaa atgctttcag
     3301 ccctttttca cgacgcagac gcaaggcttc gacgatgtaa ccaatcgcgg acttcggcgc
     3361 agtcgggttt tccagatcgt gcttgatcag cgggttattg agatccagtt gaccgcttgc
     3421 cgcatcagcg cagtagcctt tttccgtgac cgttagagag acaatcgccg tttgcggacg
     3481 cgccatcgcg ttgagaatac cttcgcagcc gtcgatttcc ggatgcagcg cttctttcat
     3541 cgaaccgata attttcagct cggtgctttc tgcgcctttt tccgctacgg tgtacagcag
     3601 ttgctgtttt ttcaggtttt cgatcagcat gcggtcgttg cctggcatca ggttaacttc
     3661 gcagatgccc cagtcgctgt cggtgctttc cagcagatga tgggtataca gcgcctggtg
     3721 cgcgcggtga aacgccccgc aaccgagatg cacaatgcgt gattccagac gagaatgatc
     3781 ccatgacggg cgggcaaccg gcagattgct gtcaacaata gtagtcatcg taaactccag
     3841 aatccgtctt cagagagtga cggcaaggaa gaaggaaccg gcacggcgcg tagccatgcc
     3901 ggtgggtatt aacggctaaa gaaagcgcgc tggatcgcca gttcgacacc gcgaacttcg
     3961 gccaggcctt tcagacgacc aattgcggag taaccagggt tggttttctt cttcaggtcg
     4021 tccagcatct gatgaccgtg gtccggacgc atcgggatca ggtcttcttt gccttccgct
     4081 ttacgacggt gttcttcttc aacaatcgct ttcaccactt cgtacatatc aacgtcaccg
     4141 ttcaggtgcg ccgcttcgtg gaaggttttc gggttatctt cacgcatggt ggagcgcaga
     4201 tgggtgaagt aaatacgcgg accgaactgc ttgatcatat caaccagatc gttgtcagca
     4261 cgcacgccgt aggaaccggt gcacatggtg aaaccgttcg ccatgctgtt tacggtatca
     4321 accatccact gcatatcttc aatggtggaa acaatgcgcg gcaagccgag gatcgggcgc
     4381 ggcggatcgt ccgggtgaac cgccatacgc acgccaactt cttcagcaac tggaataatc
     4441 gctttcagga agacggcaaa gttttcgcgc agtttggctt tgtcgatatc tttgtacagc
     4501 tccaggtgct tacggaactg gtcgagggta tacccttctt ccgcgcccgg cagaccggca
     4561 atgatgttac gcgtcagacg cgctttgtct tcgtcgctca tagtggcgaa gcgttcagct
     4621 gcctgagcaa tttcttcttc ggtgtaatcc gcttccgcgc ctggacgttt caggatatgc
     4681 atttcgaatg cagcgaattc gatctggtcg aagcgcagag ctttggagcc gtctggcagc
     4741 acgtattcga ggtcagtacg ggtccagtcg agcaccggca tgaagttgta gcacacggtg
     4801 cgaatgccac actgcgccag gttgcgcaga gtctgctgat agttagcgat ccactgctca
     4861 tagttgccag tgtgggtttt gatatcttcg tgaattggta cgctttctac gacagaccac
     4921 accaggcctg cgtcttcaac gatcgccttg cgtttgagga tctcttctac ggaccatact
     4981 tcgccgttcg ggatatggtg cagcgcggta accacgccag ttgcgcccgc ctgacggaca
     5041 tcagctaaag aaaccggatc gtttgggccg taccagcgcc aggtctgttc catgattttt
     5101 cctctttcac atagtgggtt ccacagcaaa gagacgcgga accttggagt tgtttaggta
     5161 tccggatgaa caaccagacc gcagccgtgg cgttgcatcg gatgagatgg cgtataagtt
     5221 ctaccttttg gtaagacatg tcaaccagaa ttctgaaatt ggttaaccgc atcgcaagaa
     5281 tttcactctc atggttgttg ctgcaaattg cagcaaaggg taatcgatgg ttattgatca
     5341 gaggatgtga cattcatcgc aacaatggtt gaccaattta cataacatgt cgcccaaaat
     5401 aacaacggtt caaccaagta aggtaaaacg ttatgcatgt gcttaacatt ctctgggtgg
     5461 tattcggcat tggcctgatg ctgatactga atttgaagtt caaaatcaat tcaatggtgg
     5521 ctttgttggt ggcggcgctg tccgtcggga tgctggcagg catggatttg atgtcgctgc
     5581 tgcacaccat gaaagcgggc ttcggcaaca cgctgggaga actggctatc atcgtggtgt
     5641 tcggtgcggt catcggtaaa ttgatggtcg actccggcgc ggctcaccag atagcacata
     5701 cgctgctggc gcgtctcggt ctgcgctatg tacagctgtc ggtgattatc atcggcctta
     5761 ttttcggtct ggcgatgttc tatgaagtgg cctttatcat gttagcgccg ctggttattg
     5821 ttattgccgc cgaagctaaa attccgttcc tgaaactggc gatcccggca gtagcagctg
     5881 ccactaccgc acattcactg ttcccaccgc agccgggtcc ggtggcgctg gtgaatgctt
     5941 atggcgcgga tatggggatg gtttatatct atggcgtact ggtgacgatc ccaagtgtaa
     6001 tctgcgcagg tctgatcctg ccgaagttcc tcggcaatct tgagcgccca acgccatcat
     6061 tcctgaaagc agatcaaccg gtagatatga ataatctgcc ctctttcggc gtttcgattc
     6121 tggtgccgct gatcccggcg atcattatga tctctaccac catcgccaat atctggctgg
     6181 taaaagatac ccctgcctgg gaagtggtta actttattgg ttcctcgccg attgcgatgt
     6241 ttattgcgat ggtggttgca ttcgtactct ttggcaccgc gcgtggtcat gacatgcagt
     6301 gggtgatgaa cgcttttgaa agcgcggtga agagtattgc aatggtgatt ctgatcatcg
     6361 gtgcgggtgg cgtgctgaag cagaccatca tcgacaccgg cattggcgac accatcggca
     6421 tgttgatgtc ccacggcaat atctcgccct acattatggc atggctgatc actgtgctaa
     6481 ttcgtctggc gacgggtcag ggtgtcgttt cggcgatgac cgccgccggg attatcagtg
     6541 ctgcaatcct tgatccagca accggtcagc tggttggcgt gaatccggcg ctgctggtac
     6601 tggcgacggc tgcgggttcc aacaccctca cccacattaa tgatgcatct ttctggttgt
     6661 tcaaaggtta ctttgacctg tcagtaaaag acacgttgaa aacctggggt ctgctggagc
     6721 tggtcaactc cgtggttggg ctgattattg tgctgattat tagcatggta gcgtaaaaga
     6781 acaccagagc ctgccaatgg caggctcaga ctgatgacta aagtctaaaa atgtgcccgg
     6841 acagtcccct cgcccctccg gggagagggt tagggtgagg ggaacagacc agtaaagtgc
     6901 gctttgttta tgccagatgc gacgctgacg cgtcttatct ggcctacaaa gggctaacgt
     6961 gcaggttttg taggtctgat agcgtagcgc ctcaggtacc agcattagca atgcgctgta
     7021 atttttttat tgataaacaa aagtcacgcc aataatcgat tgcacattcc ctgcagtcac
     7081 ctgccctccg gtacgtgcgt aatttgccgt taatcccaga cttaccgccg aagttcctac
     7141 tgctcctaac gataccgtgt tattcgctgg aataatcgta ccgttgcgcg tcaactgtac
     7201 gccgacgccc tgcgctggtg aaaacgacgc ggtattggtg aaaatcgagt tgcccgcatc
     7261 tgcggttgtg ccggagaggt aataccccag gttttggctt ttcgcacaat aaacggtaag
     7321 aggaattggc actgaaccag ggtagtccgg cagagtaacg gtgacatcac gagcagaaac
     7381 atcacaaccg ccagtgggca ccaccacatc attattggcg taaatattcc acacaaactg
     7441 aaaatcatcg ctgttatagt tgttggtctg tcgcaaaata agcacggcaa ttaatgaacc
     7501 agctttaatc gccaccccgc ccgcactgct cacaggcgtc aaataaagcg ccaccggcca
     7561 cggcttatcc gttctcgaat tataaacaac ccgcggcgtt tcgctggtgg tcggaaatgg
     7621 atagctactg ccactatatt ttacggtccc ggaaaaatta gataacacgc cgccgtaagc
     7681 cgagcctcgt tgcagtgtga catagtctgt aatggtttcc ggataatcgt tatggcaaaa
     7741 gatttgcgtc gaaagatcta cgaccaggtt ttgccccaca ttcacgacag gcgcaaggtt
     7801 tacataaaca ttagcgctgc caccgccaat agggatagcg gtaccattgg cggttttaca
     7861 ggcgaatgac caggcattta ccgaccagcc catcagcagt acagcaaaca gggtaatagc
     7921 tcgtttcatt acaattatct cttcgggttc agctgtaggt ataggtgatg ctaatcactg
     7981 cctgaatggt tccctgagtg gctccgccat ttaccgtcaa tgctctgacc tgtaacggga
     8041 agtgcgctga ttgtgaggaa tcatccacct gaactgtttt ggttgcgcca gtattcaatg
     8101 tgttgccact gtcatcctgt agctctaact ggatgttttg cgcggtcccc tggtttttat
     8161 aatatccggt gctgtcggct gccccgctga agctggcagt gaccctcgac gttcccaccg
     8221 gacaattagt caactcaagc gcaacatcat gccaggccga tgccgccccg gcagacataa
     8281 gactgaaaga ataaagatcg ccgagatcta ccgtggcatt ggtggtggaa accgtacacg
     8341 gtttggcgac gaccttaccg ttcaccgtga tggtgacatc ggctgcctgt atcgtcgcac
     8401 ttgcgagcgc caacattgcc gccaatacat acccacgttt gcaccatttc atgagcatct
     8461 ccagttactg atattcaaga gtgaaggtag ccgtagcatt gatatgcccc gcagtgacag
     8521 gcacctgtgt cgccattagc cgggcgtaaa aattcagcgt atttggttta cccggcgtca
     8581 gggtcgtcca cgaaatcgca gacgatggag cattaagggg tatttgattt tgctgctcat
     8641 tcagaagctg tattcccagt cccgcagccg ctgacaccgt attttcaagt gcaagcaggt
     8701 tggcattgtg gctatctgca acgccggtaa acccaacttt tacggcagaa acggcattac
     8761 cacagggtga cagcaaaata cgaaatggaa cgacaggagt cgtcgcgcca atgttgttaa
     8821 attgcttcgc cgcgttttcc atcagatcaa cagtaaaatt ggttgattca gcggccacac
     8881 tacagccgtt atctctgaca tagccgcgga tagtaatcgt gctatccgca gccaaaacgc
     8941 gacttaccgc cagccacaaa aaagcgcaca gaagataaaa aggtttgttt ctcatcacgc
     9001 ccccttaacg acattcagct gatagctggg ttaataactg ctgctgactc tctggtggca
     9061 gttgataatt ggcgacacag tgagcatttt cctcttctcc ccatttcacc tgaacttttc
     9121 ccgctaaagg cattccgctg aggtaaacct gaccattatc cgcaacaatg ccgctactct
     9181 ggctactctc tgatgtcacc atcgccccaa acggcagcgg cttattattg tgagttagcg
     9241 tcatgagcag ttttatccca acgcgcgctt taaactctgc tcgcacgatc gccccacgag
     9301 tgggaacaac gttagcgacc gcgttatcta aatcgacgtt atcagccaag gtattggtat
     9361 ccagcgccac tctattttcc cgatattcag tggcataagg cagcacggca taaccacgcc
     9421 agtcggtacg cacccccgtc tggttttcga cttttgcatc ttttgcgcca ggcgctttaa
     9481 caagcaccac cgtatcgttt aacggctgcc ccagcgttac gccattggca tgagccaata
     9541 ccccaccgct gactccgtaa tagagctgct taatatcatc gctatggctg taaccgatat
     9601 tggcattgcc gtaaccaccg cgataattca gcgtggcgta gcctgtactt ccgctattac
     9661 catcgcctcc cccggcatag ccggtttgca cgctataact gaggttgttg tcttccagca
     9721 acgtaccgta tacaccagcc agattggtca tccgaccgtt gagatcgtgt gacatgctgt
     9781 agctggcact ggcatgtcgc cactgagatt tactgtcaga acgcagccag tggctgaaag
     9841 gaatattgac gttaagcgct aacatctgat cccgtccttt ttgccaggcg tttttcgtca
     9901 ggctatagct gagcgtccag ttgatatctt cgaacgcagt atttaatcca gcctggaatt
     9961 gctcatcgac attactcgtt ccccaataag tttgatggct accactcaaa tacagtgttg
    10021 atgtgcgccc gagttgctga gtaacggtga gctgtaattt cccgcgtttg ttataagcga
    10081 ggttgtaata gtcggtgaat ttcggcttaa cctgaataac tccatcctgt gtttcgatgt
    10141 tgtagccatt cattcgactg tatgttgtat cagcgaaatt aaaatatccg ctggtcgaat
    10201 aacggtaacc cactaactga atattcgtgc ctgattcatt gagcgatttg ttatagagaa
    10261 aacgcaccga ttgtccgtca tgctgactgt catcgggaag tgtggaatta gcctgcgtca
    10321 tatccacaga cagagcgccc agtgcctcca tgtttttccc gataccgaaa ttaaaagcac
    10381 gataacgatc cgccagttgc gttccaccat atattgtcca gccagccgga aggccgtgga
    10441 gtaatgtact ctggaaaaag cggggttttt cctgctgcgc atttccacta cggtattctc
    10501 ctgccgtaat ggaataacga gtatgccctt cacgttgcaa aagcgggact gacgaatagg
    10561 gtacggtaaa aatctgcgtg ctgccgtcag cctctttgat cgttacctgc aagtcaccac
    10621 tattacctgc ggcatagata tcgttgatgg taaaaggccc cggtggcacc gtactattat
    10681 aaatgccata tccattttgt ttaatagtga cctgtgcagt accacgagca ataccgtaga
    10741 tcaccggggc aaatcctctt tggctatcgg gtaacatatt gtcatctgag gccaattgtg
    10801 cgccgcgaaa gttaatacca tcgaaaatat cgccctgggt ataaccatca cccaacgtca
    10861 gccgggaacg taaaggaatt atgtctcgct caatccaggt aatgatatgc tgccatttat
    10921 ttttgctacc tgatgaactg tcgctactgt tataactcca ggtggtattg tcgcgtaaac
    10981 gccacgcacc aatatttaac ccactctgta ggtttaaata tgcataatgg ctgttacccc
    11041 caatccgatt ctgtacacta tttccgctga aattataatt gagcaatccg gcattaatac
    11101 cgggatccca taactcagga ggaatataac cacgcgcgcg attactcata aatgcctgag
    11161 ggatcgtcag gttcagtcgc tgctgaccaa catccagatg cgcagtagcg tcttggacca
    11221 ttgtggttaa tggcacacag gcatcatccg ccagcagatt cataccggcg acagaagccg
    11281 tattcagccc catactggcg agttgcgcgc gtgtcaggca gggaacaatc ccttgttcac
    11341 tgtcgcccgt attaaatgtg acatcacgcg ttgccatata accattattc aaatagatat
    11401 cgacgcgata cgtccctggc ggtaattctt gcccattttc aaaacgcgat aaatcggcca
    11461 cagcctgggg atcatccgct aaaaagcgca gattaaaata gaggtcggca gatgacaaag
    11521 gtgcctgtgc ggcaaaagca caggcgacaa cgagtcggac aaaaaaacca gccaaacgat
    11581 gcttacgaat atgcaagcat tgtgtgtttc gctggtaaag tcttaaattc agatatgaca
    11641 ttattcctcc ggtttaaaat cacccggcag tcaattcttt ttattcaggc gaaaaattcc
    11701 ccctgcgtta ttccattacg cccgtcattt tgggggtaag tgcgccataa tcatttattg
    11761 ttcggtaagt aatattgctt cctgcatcag aaggcaattt aaccgtgctt tcgcccattg
    11821 gaggcaccaa tgcattttca agaacccggg ttccggcatt caactctgtt accgtcaggt
    11881 aatagggtgt cgggttaatc agcgtcagag aattcgcgct acgacgaaat cttaattttt
    11941 ctgcggcctg atcgggtggc aacgctaatt tagccgggcg atagtacagt ttaatgcggc
    12001 tgataattgc gagctgtagc atattctcag tcaattttga tttatccatt gacggaatcg
    12061 ctttaacgtt catccagaat aaactttccc ggtcctgtgg caattggtta tttgttgcat
    12121 caagaatacg taaggtattc tctttttttc ccttcatcgc aaacagagga ggcgtcacga
    12181 taaaacgacc atcctttaca ccatcggcat tttccaccca tgattgaatt aaataggtac
    12241 tattttcatc attatttgtc acggcaagtt gcacttgttt ttaccctgcc ggataaatta
    12301 cgcgagtcgc acctaaggcc actcctgctt cagcgcgtcc ggcaaccatc attgccatga
    12361 acatcaggat acctgccagc aagcagaatg ttatttcctg cgatttcctt acattgacgt
    12421 ttttattact cactgtcctg ttcctgttat cacattatct actgaacaat tactgatagg
    12481 ttaaagagaa ccaggcctgg gcattggcga tgccgccagt aacccgacgc ccggtagcac
    12541 gatatttggc gatgaaatgt agcgaagtag agcctgagta aagccgtttc cagtttgctg
    12601 gaggacgatt aatcggtacg aggtttcctt catcatcaaa caacgctaca ccaatattgg
    12661 aggctatccc tggcccctct cccacggaga gcacatccgg atttttacca tccgcgacac
    12721 cgtgaaacgc cacacccaca cgttcactca ccaccgtgct acattcccgt aaatgaataa
    12781 caaaaggcac cggtgcgcta tcttcccctg ccgcatgaaa ccggttactg ctgatttgcc
    12841 ccatattgac cgtcatttgt ttatcaccgg cttcaatccg gcaagtttcc gcaataatga
    12901 cgccctgaaa ttgcatattt ccgccgggca gcgtggtatt ccatttattt ccggccagag
    12961 caaacattgg cagcaacgac gctaacagaa ataatctctt ccttttcatc gttattttta
    13021 tcgcacaagg gtgggcatcc ctgcccgtaa tgacgtcctt gaacctgggt aggttattga
    13081 tactgaacct tgaaggttgc atccgcatta gctgcacccg gggttgcctc gccgattgca
    13141 taataacgcg cctggaacgg aatggtgttg gtaccgttat tcagggttgt ttgtgcacta
    13201 aatgtcgcac cgtccagcgt cagagcattg cctgttctgt ccaggatctg cacgccaacg
    13261 tttgttgcac tacctgcagc agaactctgc agagccagta catcagtacg cgtagcatca
    13321 attgccgtac ctaagaaggc aacagcggct tttgtggcaa cagtggtatc gcaatcattc
    13381 agctgaatgt taaaaccaac ggcagagctg gttgctccag cctgcttcag gctagcggta
    13441 cgaacctgtc ccaactgaac ggtttgatca acagagcctg catcaactgc gcaagcggcg
    13501 ttaacaactt cccctttaaa gtgaatggtc ccaccattta ccgtcgtagt atcggccaga
    13561 gccgctgcgg aactgaggga cagagccgac agaacaacga ttgccagagt tttaattttc
    13621 atgctgcttt cctttaaaaa aactatttct aaatcgacat gggcagtcgt tctgtacact
    13681 ttgttttgtc aacgagtttg actgccaaca ctgcacagtt ttcccccaaa agatgaaaca
    13741 tttggggcca ttttgactca tagaggaaag catcgcgcac aaactttttc agtttatttg
    13801 ttggcttaat attctattat tatctttatt tatagatttt tatattgcat gaggtagttt
    13861 ttggagagaa gaatgaggaa gatgcgtcga gccacagaaa cgttagcttt acatatagcg
    13921 gaggtgatgt gaaattaatt tacaagagaa ataatttaca tatcaaatag ttagatgctt
    13981 ttgtcgtttt ttaatatttt tatgcttgag aaaaaatacg taacttattt atgatatgga
    14041 cagtttggcc ccaattgtct tgtgtttatt tgttttttaa atttattatc aataagttaa
    14101 atcaaacctc ttctcttttt aatttttcgt ttatgagatt atttctttcc cataatccgg
    14161 caaaacgagc agcattactg gcggtataac gcacagtatg gcgaatattt cgattcccga
    14221 gataatcctg aattaaacgg gtatctgcac cacgctccgc cagttcataa ccgcaagcat
    14281 gccttaacat atgaggatga gtctgcgtta cggttccagc ttcaataccg gcatcgcgaa
    14341 taatgcgata ggcctgctgg cgagaaagcc gactcccgcg gcgagaaata aatatagcgt
    14401 cggtccggtc agcgcctttc cagttagcac gttcctgggt ccagcgttcc acggcttcac
    14461 gctcatcaaa acgtaacggg tgaacggtag aaaatccgtt cttcagtcgg cgaatattta
    14521 ttcgaccttc attaaggtca aggtcctgat aatgcagatc aagcagttca ctaatacgca
    14581 tcccatgccg atatgccaac agaataagac aataatctct ggctcccgtt gccccgtaac
    14641 aaaccgcctg catcatggcc tgaacttctt taccggtaag ataacgacgt ttactcacaa
    14701 tagtagtact cctaactgag atatatttga atgaatacct ataggaaacc tcaatcggtc
    14761 aaaattagcc caatagataa aaagataacc acacacgaag ttatgtggct atcagtcaat
    14821 tacattaaac atcatacaaa aataaaatat cactgatgtg acaacatcta ttttcattga
    14881 tttgattcaa tataacaata gattacagag gattaacttc cagttactta aaaaataaag
    14941 aaaaaagtgt ggaaacagga gaatttaagt gatgtgtaaa cttatgaatg attttgttta
    15001 tttattttga agaacataaa ccattagatt ttgtatgggt atggttaact taatgtttat
    15061 tatgataatt aagaatcaca cttacaataa catcaattaa taaatggtta atcatttttg
    15121 atatatcgta agaataatgt agtttttaac accatccctg gtatctcaac tatctctata
    15181 aaacagcatg acgctgtcgt cctctggctc tatcccagat gccgtaaaaa cgccctgcat
    15241 tgctggcggt ataccagaca gtatgacgaa tattgcgatg cccaagataa tcctagataa
    15301 gtcgcgtatc tattcccata ttcgccaaag caaaaccaca cgaatagcgt aacatgtgcg
    15361 gatgaatctc cagtgacaac ccggcattac caccagaagt cgagataata tggtaaaact
    15421 gttgccgaga aagcggattc cccttacgtg ataaaaatac ccactcgctc tcagcatgcg
    15481 gataagaagt acggatactc aaccagtttt ttaaagcctg aacttctttg ttcaatagcg
    15541 ggtgcgttgt tgaaaagcct ttttttaatc gatggatata tatacacttt gctttaagat
    15601 caatatccga aatcctcaat cgacaaattt cactcgcccg gaaaccatga ataaaacaaa
    15661 gcaaaatcag acaataatta cgtgttgcat gaggcccggt atttgctgct ttaaggagtg
    15721 actcgatttc actatgggta aggaagttcc tttttttgtt atcagcctta ttcctcatcg
    15781 ttttccctta taattacaga cgcgcactag ctgtgctggg ttagcaataa tccaacattt
    15841 ttatcctgat tatgtttata aaagcgaacg tttgcttaat aacttacgta agtggaccag
    15901 ttcttctgag cgaacttaaa tggagtagcg atagttactt ataataactt tgccatacaa
    15961 acgccatgct cttgcatgct atgtaccttt atatatttat caatctgata cgaatctaat
    16021 aataaccctc atcattaatg caactaatct tatctatata tcatgtgata tgttctgtaa
    16081 caagtaaaat caacataaaa aattcagcaa aatttaatat ttgttaaact agccgtctca
    16141 aatatgacat tattagttaa ctgcatttgc cacaaaatgg ctatttcatg gaacgcagta
    16201 atttcttagt ggctattatc atgctaattc ttagatccct agcgattatt cctgcctggt
    16261 tcgtcagctt aggccatgag attgttacat tcctttttat cctggagata tgggggaacg
    16321 caggcgacgc tttttcatct acatccgttg gtaaatatag ctttggtcac tacgacttaa
    16381 gccttcctca atcacagcta ttcgccgggc gatacggcaa acaaactaag tgaaacatca
    16441 tcctgaaaac accaacatca acaagcctct ccagatcgac atcaggagtg accagttaca
    16501 agccacaaac caagaaacat atttcatgat gagaattatg ctcagcctga tggcgagtaa
    16561 acccttattt tcaccactct cactgagtta gcttgcaaag ctttctttgt ttagattcta
    16621 aataaattat ttataaaaca tatagttaaa agaaaatctt tagtttttga agaccaccgc
    16681 aagtgttcgt ctggcttcac atggcatctt cttcttagaa aaagatcgac atattttgtg
    16741 acgcgaattg caaatctggt tttgttgtat ggattgcgtg atttttgatc tggtataaca
    16801 ggtataaaga tgcaccacga tagtcaatga gactgggcat ctcgtaatct atggcaaaca
    16861 tcacttcagt tctttcacat cgggtgatga aaatgcactt cagtctgaaa ggaatatgaa
    16921 aatgagatca acagacactc tattttatga ctctgggtaa aatgggttga gtaagtgata
    16981 taacttacga atattcaaat caattaaaaa tcagaagaga ttttatactc aggtatttaa
    17041 tctggatctc tgtttattta aataatgtta aaagagaatt ttcacaggag accttataca
    17101 aaaaatataa aatacagtta cccgttgcca aagacactat aagcctggca aaaaaatatt
    17161 acacaacata aatgctaatt gtttatgcgg gctttgtatt gctttctgta tcctacaaat
    17221 gagtgaaatt tatgaaaaag gctaaaatac tttctggcgt attattactg tgcttttcgt
    17281 ccccattaat ttctcaggct gcgacactgg acgtacgtgg tggatatcgt agtggaagcc
    17341 acgcctatga gactcgactc aaagtcagtg agggatggca aaatggatgg tgggcaagca
    17401 tggaaagtaa tacctggaat accattcatg ataataaaaa ggaaaatgcc gcactcaatg
    17461 atgttcaggt tgaagttaat tacgcaatta aacttgatga tcaatggacg gtgcgcccgg
    17521 gaatgttaac gcattttagc agcaacggca cacgctacgg accctacgta aaactgtcct
    17581 gggacgcgac aaaagatctt aattttggca ttcgctatcg ttacgactgg aaagcttacc
    17641 gacaacaaga cttatccggt gatatgtctc gtgataacgt tcatcgttgg gatggatatg
    17701 tcacttacca tattaatagt gatttcacct tcgcatggca aacgacgcta tacagcaaac
    17761 agaatgatta tcgctatgca aaccacaaga aatgggcgac ggaaaatgcc tttgttctac
    17821 aataccatat gacgcccgat attacgccat acatagaata tgactacctt gaccgccagg
    17881 gtgtttacaa cggcagagat aatttatcgg aaaacagtta tcgcattggt gtgtcattta
    17941 aactgtagta gacaggagac agtcacaatg aataaaacta taatggcgct tgctatcatg
    18001 atggcttcat ttgccgcaaa cgcgtctgta ttaccggaaa ctcctgtgcc atttaaaagt
    18061 ggtaccggag caattgataa cgacactgtc tacattggtt taggtagcgc aggtacagca
    18121 tggtacaagc tagatacaca ggccaaagat aaaaaatgga ctgcgttagc tgcattccct
    18181 ggtggaccga gagagcaagc aacctctgca tttattgatg gcaatctgta tgtgtttggc
    18241 ggcattggca aaaacagcga gggattgact caggtattta atgacgtaca caaatacaac
    18301 cccaaaacca atagctgggt taaattgatg tcgcacgcgc cgatgggcat ggcgggtcat
    18361 gtgacttttg tacacaacgg caaggcttat gttactggcg gtgttaacca gaatatcttc
    18421 aatggctatt ttgaagatct caacgaggct ggaaaagatt caaccgctat agataaaatc
    18481 aacgcccact attttgacaa aaaagcagaa gattatttct tcaataagtt tctgttgtct
    18541 tttgatccct caacacagca atggagttac gctggcgaat cgccctggta cggaacggct
    18601 ggtgcggcgg ttgtgaataa aggtgataaa acctggctta ttaatggcga agccaaacca
    18661 ggattgcgaa cggatgccgt atttgaactt gatttcaccg gtaataattt aaaatggaat
    18721 aagcttgatc ccgtctcatc accagatggc gtcgctggcg gttttgcggg gataagcaat
    18781 gattctctta tatttgccgg aggggccgga ttcaaaggtt cacgagaaaa ttaccagaac
    18841 ggtaagaact atgcgcatga aggcctgaaa aaatcatata gcactgatat tcatctttgg
    18901 cataacggga aatgggataa atcgggtgaa ttatcgcaag gtcgggccta cggagtatca
    18961 ttgccctgga ataatagttt attaattatt ggcggtgaaa ctgcaggcgg caaagcggtg
    19021 acggattcag ttttaatttc tgtgaaggat aataaagtca cagtacaaaa ctaacgcttc
    19081 agggccccgg taaaggggcc ctgctatcaa tttgtcattt gtattacgcg aaaattatat
    19141 gaacgcaata atatcacccg attattacta tgttcttacc gttgctggtc agtctaatgc
    19201 catggcgtat ggcgaaggac tgccattacc ggacagggaa gatgcgcctc attccagaat
    19261 taaacaatta gcgagatttg cgcatacgca tcccggaggc ccgacatgtc actttaacga
    19321 cattattcca ctgactcact gcccacacga tgttcaggat atgcagggtt atcaccatcc
    19381 tctggcaacg aatcatcaaa cacagtacgg caccgttggc caggcactgc atattgcacg
    19441 gaaattactg cccttcattc ctgataatgc agggattctt atcgttccgt gttgccgtgg
    19501 cggatcggct tttaccgcgg gcagcgaagg gacatattca gaacggcacg gagccagcca
    19561 tgatgcttgt cgttggggaa cggatactcc gctgtaccag gatttagtca gcagaacgcg
    19621 agccgcactg gcaaaaaatc cgcagaacaa attcctcggc gtatgctgga tgcaaggcga
    19681 atttgactta atgaccagtg actacgcgtc acaccctcaa cactttaatc atatggttga
    19741 agcctttcgt agggatctaa aacaatacca ttctcagctt aataatatta ctgacgcgcc
    19801 gtggttttgc ggcgatacca cctggtactg gaaagaaaat ttcccgcatt cgtatgaagc
    19861 tatttatggc aattatcaaa ataatgtttt agccaatatt attttcgtcg acttccagca
    19921 acaaggtgaa agaggactga cgaacgcgcc tgatgaagac ccggacgatt taagcacggg
    19981 atattacggt tcagcgtacc ggtcaccgga gaactggacg acggcactgc gaagcagtca
    20041 tttcagcacg gcagcccgtc gggggattat ttctgacaga tttgtagaag caattttgca
    20101 gttttggcgc gaaaggtgag cggggagttt attcatgggg gatgtccttg ttcgttctca
    20161 gagtagacaa caaaaaacaa atgagatcaa ggtaatatct cttcacttgc gggctcgaac
    20221 agcaggcaat tgccaatata ataatctggc acaagggtga aaaacgcgca ccgcataaac
    20281 cgttgttatt gcttacagat tagccggatg ccttaataga catccgcgcc ttttcgatta
    20341 tggctcggaa atctacgaac atctacacag tttactggaa cgttttgggc cacagcgttc
    20401 gcagcaccgt cctgatatgc cgttctggcg attataagtc gacggattct ggcaaataga
    20461 cgatgatgat atgcttaatg atgttcaaag aaattaggcc attattatat tattgcttgc
    20521 attttctttc attcctaaaa gcacccgcca ttaatcccag gcgcattat
//
LOCUS       DAJTQY010000072        20489 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.072, whole genome shotgun sequence.
ACCESSION   DAJTQY010000072 DAJTQY010000000
VERSION     DAJTQY010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20489
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.072"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            113..1168
                     /gene="phoE"
                     /locus_tag="OO349_002708"
     CDS             113..1168
                     /gene="phoE"
                     /locus_tag="OO349_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HCR5867720.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDTVAVGMTYQF"
     gene            complement(1207..1608)
                     /gene="crl"
                     /locus_tag="OO349_002709"
     CDS             complement(1207..1608)
                     /gene="crl"
                     /locus_tag="OO349_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HCR5867721.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPANDFRDEPVKLTA"
     gene            complement(1666..2910)
                     /gene="frsA"
                     /locus_tag="OO349_002710"
     CDS             complement(1666..2910)
                     /gene="frsA"
                     /locus_tag="OO349_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HCR5867722.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPKLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            complement(3002..3460)
                     /gene="gpt"
                     /locus_tag="OO349_002711"
     CDS             complement(3002..3460)
                     /gene="gpt"
                     /locus_tag="OO349_002711"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCR5867723.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3722..5179
                     /gene="pepD"
                     /locus_tag="OO349_002712"
     CDS             3722..5179
                     /gene="pepD"
                     /locus_tag="OO349_002712"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HCR5867724.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            complement(5236..5850)
                     /locus_tag="OO349_002713"
                     /pseudo
     CDS             complement(5236..5850)
                     /locus_tag="OO349_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(5912..6955)
                     /locus_tag="OO349_002714"
     CDS             complement(5912..6955)
                     /locus_tag="OO349_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HCR5867725.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSAGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGLGQSILQRHIASFSHHGLPEGSDDALAFARFNRHLIALRIMQQVK
                     ATGSPVLDVAHNFVSACRIGDQQGVLHRKGATPDDCGLVVIPGSRGDYSRLVQPVRSE
                     ETLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQLIFEEAPQAYK
                     SAESVVQCLGRPD"
     gene            complement(7201..7653)
                     /locus_tag="OO349_002715"
     CDS             complement(7201..7653)
                     /locus_tag="OO349_002715"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5867726.1"
                     /translation="MNNIQIRDYHPGDFQQLCAIFIRAVTMTVSQHYSLQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPFIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGAWFINFYMRYKPQH"
     gene            complement(7663..8061)
                     /gene="yafO"
                     /locus_tag="OO349_002716"
     CDS             complement(7663..8061)
                     /gene="yafO"
                     /locus_tag="OO349_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HCR5867727.1"
                     /translation="MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDEAHLVYCQGAFDEQAWLLIA
                     ILKPEPHKLARDNNQMHKIGKMAEAFRMRF"
     gene            complement(8064..8357)
                     /gene="yafN"
                     /locus_tag="OO349_002717"
     CDS             complement(8064..8357)
                     /gene="yafN"
                     /locus_tag="OO349_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HCR5867728.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRAKQYLNDMTDDDFNDFKE"
     gene            complement(8409..9464)
                     /gene="dinB"
                     /locus_tag="OO349_002718"
     CDS             complement(8409..9464)
                     /gene="dinB"
                     /locus_tag="OO349_002718"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HCR5867729.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLEKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(9535..10320)
                     /gene="lafU"
                     /locus_tag="OO349_002719"
     CDS             complement(9535..10320)
                     /gene="lafU"
                     /locus_tag="OO349_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HCR5867730.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLYNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRMR"
     gene            10292..12004
                     /locus_tag="OO349_002720"
                     /pseudo
     CDS             10292..12004
                     /locus_tag="OO349_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(12221..12718)
                     /gene="rayT"
                     /locus_tag="OO349_002721"
     CDS             complement(12221..12718)
                     /gene="rayT"
                     /locus_tag="OO349_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HCR5867731.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSHLLTTQFQTLRNAIINVKR
                     DRPFEINAWVVLPEHMHCIWTLPESDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPEKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDITDLSAG
                     ERIIL"
     gene            complement(12894..13415)
                     /locus_tag="OO349_002722"
                     /pseudo
     CDS             complement(12894..13415)
                     /locus_tag="OO349_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414762.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
     gene            13799..14539
                     /locus_tag="OO349_002723"
     CDS             13799..14539
                     /locus_tag="OO349_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HCR5867732.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVAN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(14510..15277)
                     /locus_tag="OO349_002724"
     CDS             complement(14510..15277)
                     /locus_tag="OO349_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HCR5867733.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRAKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWHLFCLGERVV"
     gene            complement(15483..16061)
                     /gene="lpcA"
                     /locus_tag="OO349_002725"
     CDS             complement(15483..16061)
                     /gene="lpcA"
                     /locus_tag="OO349_002725"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HCR5867734.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            16301..18745
                     /gene="fadE"
                     /locus_tag="OO349_002726"
                     /pseudo
     CDS             16301..18745
                     /gene="fadE"
                     /locus_tag="OO349_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
     gene            complement(18788..19234)
                     /gene="ivy"
                     /locus_tag="OO349_002727"
     CDS             complement(18788..19234)
                     /gene="ivy"
                     /locus_tag="OO349_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HCR5867735.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     AIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            19415..20185
                     /gene="yafV"
                     /locus_tag="OO349_002728"
     CDS             19415..20185
                     /gene="yafV"
                     /locus_tag="OO349_002728"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HCR5867736.1"
                     /translation="MPSLKITLLQQPLVWVDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHKATRIDAELSMVALREYREKFPAWRDADEFRLW"
     gene            20295..20489
                     /locus_tag="OO349_002729"
                     /pseudo
     CDS             20295..20489
                     /locus_tag="OO349_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000541010.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SymE family type I addiction module toxin"
BASE COUNT         5010 a         5265 c         5270 g         4944 t
ORIGIN      
        1 gaatatcatt aatctgtaat atatctttaa caatctcagg ttaaaaactt tcctgttttc
       61 aacggggctc tcccgctgaa tattcgcgcg ttaattaaaa tcaggaatga aaatgaaaaa
      121 gagcactctg gcattagtgg tgatgggcat tgtggcatct gcatctgtac aggctgcaga
      181 aatatataat aaagacggta ataaactgga tgtctatggc aaagttaaag ccatgcatta
      241 tatgagtgat aacgccagta aagatggcga ccagagttat atccgttttg gttttaaagg
      301 tgaaactcaa attaacgatc aactgactgg ttatggtcgg tgggaagcgg agtttgccgg
      361 aaataaagcg gagagtgata ctgcacagca aaaaacgcgt ctcgcttttg ccgggttgaa
      421 atataaagat ttgggttctt tcgattatgg tcgtaacctg ggcgcgttgt atgacgtgga
      481 agcctggacc gatatgttcc cggaatttgg tggcgattcc tcggcgcaga ccgacaactt
      541 tatgaccaaa cgcgccagcg gtctggcgac gtatcggaac accgacttct tcggcgttat
      601 cgatggcctg aacttaaccc tgcaatatca agggaaaaac gaaaaccgcg acgttaaaaa
      661 gcaaaacggc gatggcttcg gcacgtcatt gacatatgac tttggcggca gcgatttcgc
      721 cattagtggg gcctatacca actcagatcg caccaacgag cagaacctgc aaagccgtgg
      781 cacaggcaag cgtgcagaag catgggcaac aggtctgaaa tacgatgcca ataatattta
      841 tctggcaact ttctattctg aaacacgcaa aatgacgcca ataactggcg gctttgccaa
      901 taagacacag aactttgaag cggtcgctca ataccagttt gactttggtc tgcgtccatc
      961 gctgggttat gtcttatcga aagggaaaga tattgagggc atcggtgatg aagatctggt
     1021 caattatatc gacgtcggtg ctacgtatta tttcaacaaa aatatgtcag cgtttgttga
     1081 ttataaaatc aaccaactgg atagcgataa taagctgaat attaataatg atgatactgt
     1141 cgcggttggg atgacgtatc agttttaatg aatattgccg gatgtgatgc atccggcaca
     1201 tttcactcac gccgttaact tcaccggttc atcacgaaaa tcattcgccg gttccagctt
     1261 cagattcagc gtcgtcagca gttcacgcag cttctcgtga aactcacgca gggtgtgctc
     1321 cagtcgttca accacttcag tatcttttac cggaacactc ttccagtcgc ctgctttatc
     1381 gaacagacca aactggtagc tgtaggtaaa acgggattcc tgcgcttcca gctccatcca
     1441 ccagccccag aattcacgca cttccggtgc cggtttcacg ttgacgcata cagccagaca
     1501 atcgaaaaag aatcgattat cttcgcactt accttcacga atatacgggc ctagtgcggt
     1561 aaattttttg atcaatctgc tcttcgggtg tccactcggt aacgtcattg ctatctcctg
     1621 ttgtgatgca actgttttac caaattggca aaatttagca aatttttaac acaagcgttt
     1681 ttcgatccag ccggtgattt cctgaagacc tttgtcaaaa ttccgataca ccgggttaaa
     1741 tgggatctct aataatttac cgtcagcaga tgatgaggtg attaagcgtg agtcctcttc
     1801 cgggctgaac ggatcgttct tccagtagcc tgataacatt ggcgttgggc agcgacgccc
     1861 cagcaatcct tgcactttta atgaatagcg attcagctcc acgcgtaacg cttcgtcgga
     1921 agcatcatgc atccccaaac gactcgccag aacgtcaaga tacatttccg gcacctgttg
     1981 ctggcactta aaatcactca acagggtatg aactaccgga ccaagacagg caaccgcttt
     2041 cagacgcggc gattcaaggt atgccagacg cacggcaacg ttagcgccga aacggaaacc
     2101 aaaggccgcg acgcgagtgt gatccaccca cggtacgtta ggcagcgcct ttaagacgtg
     2161 ctgatgcaac aggctggagt cctgggtgag cttccatttt gaagaaaagc ccaccgacgg
     2221 catatcaata gtcagcatcg caatgccgcg cggcgcaaaa taacgttcat acaggctgta
     2281 atagtccgtc tgcatcgcat ccagaccacc acacattaat actgtcggga acgggccatc
     2341 gcctttcggc atatgcaaaa agccggtgat gggcgcaccg ccgggtacgg taaactccat
     2401 ctgccgcatc gtgcccggca gacgctgggc ggcctcttca taggcgcggt ttgataaagc
     2461 ctgcgcttgc tcggccaggt catctccttt cagatgagga taggcggcaa tgttgtacaa
     2521 cgtagccgca tgcagccagt gtcgaccact gagtttcgga tcttcttccg tacaggcttt
     2581 ttgttgccac accatcgcct gggtggccca ctcataaatc cagttgccac cacggtagcc
     2641 aatcacggta tcgtataaat catcgtcggt acgttcagca tcgctcatca caatacgtgc
     2701 ctggacgtcg aggatttcac gtgggtcaat accgcgccag atccacatca gacggttaat
     2761 catgcgatac cagtgaggga tggttttacc atcaagagcc gactgcacag gcggttgtgc
     2821 gccgtgatta aagcggcgga ctagcgtcga ggtttcagga tgtttaaagc ggggtttgaa
     2881 cagggtttcg ctcaggtttg cctgtgtcat ggatgcagcc tccagaatac ttactggaaa
     2941 ctattgtaac ctgcctgaag ttaaaaagaa caacgcccgg caatgccagg cgttgaaaag
     3001 attagcgacc ggagattggc gggacgaata cgacgcccat atcccacggc tgttcgatcc
     3061 aggtatcttg cgggatatca acaacatagt catcaaccag cggacgacca gccggttttg
     3121 cgaagatggt gacaaagtgc gcttttggat acatttcacg aatcgcaaca gcagtaccac
     3181 cggtatccac caggtcatca ataacgatga agccttcgcc atcgccttct gcgcgtttca
     3241 gcactttaag ctcgcgctgg ttgtcgtgat cgtagctgga aatacaaacg gtatcgacat
     3301 gacgaatacc cagttcacgc gccagtaacg cccccggtac cagaccgcca cggcttacgg
     3361 caataatgcc tttccattgt tcagaaggca tcagtcggct tgcgagttta cgtgcatgga
     3421 tctgcaacat gtcccaggtg acgatgtatt tttcgctcat gtgaagtgtc ccagcctgtt
     3481 tatctacggc ttaaaaagtg ttcgagggga aaataggttg cgcgagatta tagagatctg
     3541 gcgcactaaa aaccagtatt tcacatgagt ccgcgtcttt ttacgcactg cctctccctg
     3601 acgcgggata aagtggtatt ctcaatacat atctcgcaag cctgtcttgt gttgacaaca
     3661 ttttctgcta accctgtgac ctgcaatact gttttgcggg tgatcgacaa ggagacttaa
     3721 cgtgtctgaa ctgtctcaat tatctccaca gccgctgtgg gatatttttg ccaaaatctg
     3781 ttctattcct cacccgtcct atcatgaaga gcaactcgct gaatacattg ttggttgggc
     3841 aaaagagaaa ggtttccatg tcgaacgcga tcaggtaggt aatatcctga ttcgtaaacc
     3901 tgctaccgca ggtatggaaa atcgtaaacc ggtcgtctta caggcccacc tcgatatggt
     3961 gccgcagaaa aataacgaca ccgtgcatga cttcacgaaa gatcctatcc agccttatat
     4021 tgatggcgaa tgggttaaag cgcgcggcac cacgctgggt gcggataacg gcattggtat
     4081 ggcctctgca ctggcggttc tggctgacga aaacgtggtt cacggcccgc tggaagtgct
     4141 gctgaccatg accgaagaag ccggtatgga cggtgcgttc ggcttacaga gcaactggtt
     4201 gcaggctgat attctgatta acaccgactc cgaagaagaa ggtgaaatct acatgggttg
     4261 tgcggggggt atcgacttca cctccaacct gcatttagat cgtgaagcgg ttccagctgg
     4321 ttttgaaacc ttcaagttaa ccttaaaagg tctgaaaggc ggtcactccg gcggtgaaat
     4381 ccacgttggc ctgggtaatg ccaacaaact gctggtgcgc ttcctggcgg gtcatgcaga
     4441 agaactggac ctgcgcctta tcgatttcaa cggcggcaca ctgcgtaacg ccatcccgcg
     4501 tgaagccttt gcgaccattg ctgtcgcagc tgataaagtc gacgcactga aatctctggt
     4561 gaatacctat caggagatcc tgaaaaacga gctggcagag aaagagaaaa atctggcctt
     4621 gttgctggac tctgtagcga acgataaagc tgccctgatt gcgaaatctc gcgatacctt
     4681 tattcgtctg ctgaacgcca ccccgaacgg tgtgatccgc aattccgacg tggcaaaagg
     4741 tgtggtcgaa acctccctga acgtcggtgt ggtgaccatg actgacaata acgtagaaat
     4801 tcactgcctg atccgttcac tgatcgacag cggtaaagac tacgtggtga gcatgctgga
     4861 ttcgctgggt aaactggctg gcgcgaaaac cgaagcgaaa ggcgcatatc ctggctggca
     4921 gccggacgct aattctccgg tgatgcatct ggtacgtgaa acctatcagc gcctgttcaa
     4981 caagacgccg aacatccaga ttatccacgc gggcctggaa tgtggtctgt tcaaaaaacc
     5041 gtatccggaa atggacatgg tttctatcgg gccaactatc accggtccac actctccgga
     5101 tgagcaagtt cacatcgaaa gcgtaggtca ttactggaca ctgctgactg aactgctgaa
     5161 agaaattccg gcgaagtaat tatttgattt gctgccggat ggcgtttaat cgccttccgg
     5221 cagtttcatc cttcattatc cttcgataaa agccatccct gtaaacgttc gtcgcgggtt
     5281 gccacgttca atctggtgat ggaacattcg ccgctgtgat ttcagcaccg cgctattttc
     5341 ctattgctgc tgatccagct tccaggcaat cagcaatcgc gccagccgtt tgttagcatg
     5401 ctggctgcgc tctgactgaa ccttcacgct aataccggat gccaaatgcg tggcgcgtac
     5461 cgccgagtcg gttttattga catgttgacc gcccggcccc gacgaacgca gcgtctcata
     5521 ccggatcgca tcggattgct cctgctcatc agcggtaaaa cgcccaatgc ccagaaacca
     5581 gtttttacgc ccgtgatgtg ggcgatacgg gctcgggcaa atccactgaa tagtgccaca
     5641 ccagctttcg cttaacgccc atgcgttgtc gccatccaga gaaatcagcg ccgaacgcag
     5701 cgtgtcagag tagcggcctg tttctgtttc cagcaccgtt accgcgacgt cttgtcgggc
     5761 agcttcttta attagcctgt ccagcgcctt tttcactgcg agacaacatt cttccggccc
     5821 ctgcgctgag gagagttata gcaagatcat ccttttttcc ctccactgtt tttgagcgtt
     5881 agcaccggac gcagccgcgc gacaggaata attaatccgg cctgcccaga cattgcacca
     5941 cactttcagc cgatttatag gcttgtggcg cttcttcgaa gatgagttgt ttatcgcgac
     6001 aaattacccg actgccaagt tcagtacgcg agagttgcgt cgctgtgtat ttcgctgcca
     6061 gacgcccttt acactcggtg cgcccccatt tacgcccagc cccatgcgcc agcgaatgca
     6121 atgtttcctc actcctgacg ggctgaacca gcctggagta atcaccgcgt gaaccgggaa
     6181 taaccaccag accacagtca tcaggcgtgg cacctttacg atgtaagacc ccttgttgat
     6241 caccgattcg acacgcgcta acaaagttat gcgccacatc cagaaccgga cttccggtgg
     6301 ccttaacctg ttgcattatg cgcaaagcga tcaggtggcg attaaaacgc gcaaacgcca
     6361 gcgcgtcgtc actgccttca ggcaaaccat gatgcgaaaa cgaggcaata tgccgctgta
     6421 aaatagactg ccctagcccc ctcgagccgc tatgaaccag cagttgcaga tgctgcgcat
     6481 ccagacctgc cagtgcaaac agttcagcgt tgatgatttg atcaacttgt tgcagttcag
     6541 cgaaatgatt gccaccaccg atagagccaa gcgagctgcg ccacggatgc tgtgcaaatg
     6601 ctgacggcag gttttcctcc agccagcttt cttcagcaac gtcatccaaa gctgataatc
     6661 gcttttcaaa cttatcggcg ttgtatttgc gagcgagaat atctgtttgc catagcgcca
     6721 tgccacagcc gatatcattg ccgaccagtg ctgggtaaaa acggcctgcg gagaagaacg
     6781 ctgcgccaat cgggtagccg cgtccggggt gtaaatctgg catccccact acgcgctgca
     6841 tgttgggtaa atttgccgtg gtgtgtaatt gttggatcgc taaactctcg atccacaggt
     6901 catcagatgc gatggtaaat accgcatcgg ataagggacg aatataattg cccatatacc
     6961 attccgtaaa tagactcgtc atacttcaag ttgcatgtgc tgcgacttga attattgaga
     7021 gtaacaatta aaaaatcagg aaatggcaga cgaatccagg gcaagaaaaa aagaattaat
     7081 cctggaaagg tctgcaaaga gtcattgata aaggatttgt catgatttac ctccttgcag
     7141 tttgtggaat aagaaaacgc gcacatacta ggcgggcgtt attttcattg caagctggat
     7201 ttaatgttgc ggtttataac gcatataaaa attaataaac cacgctcccc ggcattcaac
     7261 gcgctgctgc ttaactgtct gaaaaccata acgttcaaaa aagggttttg cggttatgct
     7321 tgcgtccacc gtaagttcgg attcagactt aataaaaggt tttaacaaag cgctggcaac
     7381 cccacggcgg gtgtattcag ggtcaacaaa taacatatcg atatagtgtt caacgcaggt
     7441 aataaagccg accagttttg cattaataac tgcaacccgc acttgtgatt tcgcgagttt
     7501 ctccttccag cgagattcgt caatctgcgc ccaggcggca atttgttgta gtgaataatg
     7561 ctgactgacg gtcatcgtaa ccgctctaat gaaaatagcg catagttgct gaaaatcgcc
     7621 aggatgataa tctcttattt gtatgttatt cataatataa attcaaaaac gcatgcgaaa
     7681 cgcttctgcc atttttccaa ttttatgcat ttggttgtta tctcgagcca gtttatgagg
     7741 ttcaggcttc agaatggcaa tgagcaacca tgcttgctca tcaaacgcac cctgacaata
     7801 taccaaatgc gcttcgtcat tcgttctgct gaattggcgc aactgtggcg gaaatggatt
     7861 attcacattt gccagatgaa tatgagcaac tcgctcaaat ttgattaatg gccaggtaaa
     7921 cgagtcgtcg tagagtgcat cgcgaccaaa tatatctggc aaaacaccgt cacgcttata
     7981 ggaaataaaa tccgccgtta acgcatcaag ttcctctgct gtaagttgca ggcgaataag
     8041 ttttgttttg aatacccgca tccttattcc ttaaagtcat tgaaatcatc atccgtcata
     8101 tcattaagat attgtttagc gcggcgtgta atttcctcta atcttgcagc acttggacga
     8161 aagcgcgcct ttatgggctt tttctcctct tgttcagcaa gcatgtccat taacgcttcg
     8221 aatgcgctgg cacttaagag atatcctgcg gggcgattat tagaaagaac cgcaactggt
     8281 tgatcaataa agtatttagc tgggttttta cgtaactcag tgatattgac cgatttttca
     8341 gcgagaattc gatgcataca gtgataccct cataataatg cacaccagaa tatacataat
     8401 agtatacatc ataatcccag caccagttgt ctttccattt gcgggtcaag caacgtcaca
     8461 tgcagcccca ccagacgcac accgcgcccg ccgcggcgtt catcccaggt tttacgcgcg
     8521 gtggcgatta agtcagcttt attcagccgc ggccagacgt gctcctgggt ggtctgctga
     8581 aaatcgtcga actttaattt caccccctgg cgagcaatca gtaaatcagg ttttaccttt
     8641 gccagacggc gttcaagttc cggatacagc cgctcgataa tcgcttcaca ttcagaccag
     8701 tgatgaatat cttccgccat cgtgcgttcc acgccgacgg attttcgtaa ccgttcgctg
     8761 ttaacgtcgc gctcgtcaat cccctgacta cgctcccaca aaatgcggcc aaatttgcca
     8821 aagcgtttaa gcagaatcac cagatcacac ttttgtacat caccgcaggt ccgcagcccc
     8881 atcgcttcca gttttgccgc cgagactttg ccgacgccgg ggattttttc cagtggtaag
     8941 gtttgtaaaa atgccggaac ttctgccggc gtaatcacaa actgaccgtt gggtttattc
     9001 atgtcggagg cgattttggc gagaaacttt actggtgcca cgcccgcaga tgccgtcagt
     9061 tgcagctcgt tgaagatcgt ctggcggatt tcctgggcga tgagggtcgc agaaccgtgg
     9121 caatggacgc tatcggtgac atcgagataa gcctcatcca gtgacaacgg ttcaatgcgc
     9181 gaggtgtagc gcgagaagat ttcacggata tgatttgagg cttctttgta ggcgtcaaag
     9241 cgccccggaa gcaaggtgag atgtgggcat aatttgagcg ccatcccagt aggcatagcg
     9301 ctacgtacgc caaatttacg cgcgggataa ttggcggtgc tgatcacccc ccgacgttcg
     9361 cggctgccgc caatagcaat agggatatcg cgcagagcgg gattgtcgcg catctccacc
     9421 gctgcgaaaa agcagtccat atccacatga atgattttac gcattgctca cctctcaaca
     9481 ctggtaaagt atacagtgat ttcaggtttt gagaaatgcg taaagattca gcatttagcg
     9541 cattcgctgc gaaagcacct gctgcttatc cagcttttgc accagcggct gcaccacttt
     9601 atccccatgc tgaccaaagt attgatacag cgtatcggac gcacttttgg tcagcaccat
     9661 aatctcaata cgccgattgc ccgcgctttg cggattcttc gcgtccagca gcatctggtc
     9721 cgccattgcg cttacctgca tcactttatc ttccggcatt ccggcctctt ccagcacccg
     9781 acgagctgaa agcgcgcgat cgcccgaaag gttccagttg ttgtagatat tgtttttgta
     9841 ggccatcgca tcggtatgcc cggtaataat aattttgtta tagagcgagt cgaacactgg
     9901 cgcgagctcc accagcaaag ttttaaagaa cggcataatc tgcgcgctgc cgcgttcgaa
     9961 catattgcga ttctggtcgt ctttaatcag cacgcgtaat ccctggggaa caatctccat
    10021 ctccagattc gcttccatat gcgcatcacg ggcaatggtg ttgatactgg ttgccaactc
    10081 gccaagctcg gtggcggatt tcttcttcag cagcgccgtt ttctcattca catcacgcgc
    10141 ttttttctcc gtctcttccg gcaccgccac ggtggcaggt tttggcggat gagacgggct
    10201 gattttattt aacggcgaca gcccaccacc gttaaaaatc gactgcccgt gcagcgcggc
    10261 gataatgctt tcacgctcgg atttactgac gctgttgaca atcaacttta tcgtcgtcac
    10321 caaagaggcc gagcgtattt ccgaggtttc tgcccgcttt accctggacg cgatgcccgg
    10381 caaacagatg gcgattgacg ccgatcttaa cgccggattg atcaaccagg cgcaggcgca
    10441 aacccggcgt aaagatgttg ccagcgaggc cgatttctac ggcgcgatgg acggggcatc
    10501 gaagtttgta cgcggagacg ccatcgccgg gatgatgatt ctggcgatca acctgagcgg
    10561 cggcgtctgt atcggcatct tcaaatacaa cctgagcgcc gacgccgcct tccagcaata
    10621 cgtgctgatg accatcggcg acggcctggt ggcgcagatc ccttccctgc tgctctccac
    10681 cgcggcggcg attatcgtca cccgcgtcag cgacaacggc gatatcgccc atgacgtgcg
    10741 ccaccagttg ctggcaagcc cgtcggtgct ctacaccgcc accgggatca tgttcgtgct
    10801 ggcggtggtg ccggggatgc cgcatctgcc gtttttgctg ttcagcgccc tgcttggctt
    10861 taccggctgg cggatgagca aacgcccgca ggcggcggag gcggaagaga aaagcctcga
    10921 aacgctgacc cgcaccatca ctgaaaccag cgaacaacag gtcagttggg aaactattcc
    10981 gctgatcgag cccatcagct taagcctcgg ctacaagctg gtggcgctgg tggacaaagc
    11041 gcagggcaac ccgctcaccc agcggattcg cggcgttcgg caggtgattt ccgacggtaa
    11101 cggcgtgctg ctgccggaga tccgcattcg ggaaaacttc cgcctcaagc ccagccagta
    11161 cgctattttc atcaacggca ttgaggctga tgaagcggat attccggcgg ataaactgat
    11221 ggcgctgccc tccagcgaaa cctacggcga gattgacggc gtgctgggga acgacccggc
    11281 gtacgggata ccggtcacct ggatccagcc tgcgcagaaa gcgaaggctc tgaatatggg
    11341 gtatcaggtg atcgacagcg ccagtgtgat tgccacccat gtgaacaaga ttgtgcgcag
    11401 ctatattcct gatttgttta actatgatga catcacgcag ttgcataacc gtttgtcgtc
    11461 gatggcaccg cgcctggcgg aggatttaag cgcggcgctc aattacagcc aattgctgaa
    11521 agtgtaccgg gcgctgctga ccgaaggcgt ttccctgcgc gatatcgtca ccatcgccac
    11581 cgtgctggtc gccagtagca cggtgactaa agatcatatt ctgctggcgg ccgatgtgcg
    11641 cctggcgctg cggcgcagca ttactcatcc gttcgttcgc aagcaggagc tgacggtgta
    11701 tacgctgaat aatgagctgg aaaatctgct gaccaacgta gtgaatcagg cgcaacaggc
    11761 cggcaaagtg atgctcgaca gcgtgccggt tgacccgaat atgctcaacc agttccagag
    11821 cacgatgcca caggtgaaag agcagatgaa agcggcgggg aaagatccgg tgctgctggt
    11881 gccgccgcag ctacgccctt tgctggcgcg ttatgcaagg ttgtttgcgc cggggctgca
    11941 tgtgctgtcg tataacgaag tgccggatga gctggagttg aagattatgg gggcgttgat
    12001 gtaagaatcc ggcatcggtg cacaattgcc ggatgcggcg taaacgcctt atccggccta
    12061 ccgttcaggc acaagttttt aggcatgata agacgcgcca gcgtcgcatc aggcatctgc
    12121 gcataactgc cggatgcggc gtgaacgcct tatccggcct accgttaaag cacgatttgt
    12181 aggccggata agcgtaagcg catccggcac cagcgcacag ttataaaatg atacgctccc
    12241 cggcacttaa atccgttatg tctcccgccc aatcgatggg atataaccct cgcgcgacat
    12301 cgcgatggaa cgttgagaat ggccaatcac tcacctgctt tacccagcca tgtttctctg
    12361 gatttatata aatataatca acatgatggc gataatcttt tgtattgcga atggcatgct
    12421 cccaaaaacg cggttgccag atatttttca atccgcaagc atgggtgaac tgctttttaa
    12481 tctcccgcca gcgagaggaa aaatcatcat cgctttcggg taatgtccat atgcaatgca
    12541 tatgctccgg caaaacgacc caggcgttaa tttcaaaagg tctgtctcgc ttaacgttaa
    12601 taatggcgtt acggagcgtc tggaactggg tggtcagaag atggcttcga cgattgcgta
    12661 aatttaccgt gaaaaaccat gttcccccct tgatgtaata acgacgatat tccgacatag
    12721 cgactccttt ttctcaacgg taagaggaaa aggaaaggcc gacaatgacg ctgtgttata
    12781 tcgggaaagc ggctcgcaaa aacgcacaga tttgtaggca tgataagacg cgtcagcgtc
    12841 gcatcaggca tccgtgcaca accgccggat gcggcgtaaa cgccttatcc ggcctacaaa
    12901 atcatctctt ccgtcaaaat cctgcgcgcg cctaaaaaat ggtcctgcca gaaaggttcg
    12961 gctaaccggc ttacccgaat ggtttccccg gtgcgcagtg actcaataaa ctgaccgtcg
    13021 cccagataca cccccatatg gtcggcaatc tcgcggctgt ggatatggaa aaatagcaaa
    13081 tctccccggc gcaggtcgtt attcgccaca atcgttgccc gacggtagtg gtacatctca
    13141 ttggccgtgc gcgggagttt cgcctcaagg atcttgttgt aggcataaaa aactaaccca
    13201 ctacaatcga agccttgatc gggccgcgtg ccgccccaga cgtaaggctt gccaagctgc
    13261 tgctccaggc ggtgaatcgc cacttcggtg atgttatgca ggtgatcgct gcttaaaaag
    13321 tggttgtttt ccgccagcgc ctgccagcgt ctgtcactcg ccttgagcgg cgcaggaaac
    13381 cattctggat gctgttttat ctgctcccgg ttgtggtggc gctggcgagt aaagggagca
    13441 aaacgcataa cgaaaacacg cctggatgca aaaatctgcc gagcaagaac gaagacatga
    13501 aagacataaa ccggctgaat gaaggtgaga attttaaaag accggaggag taaacgggat
    13561 tattaattgc ttatttgcat attactattg agatttcata atattaggaa ataaatgata
    13621 catgcgcttt tcggactatt gaaaactgcc cctgtgctaa atttgtaaac ccagcgccga
    13681 caggcttgcg taaaactctt ttggtgataa tgtgagcctt ccaaatccag actattccga
    13741 tttgggatcc attttgccgt cctggcgtgt gtaaccgttt tatcaaggaa taagcagtat
    13801 gcgtaaaatc gcattaattc ttgcgatgtt gttgatcccg tgcgtttcgt ttgccggttt
    13861 gctgggcagc agtagctcga ccacgccagt gagcaaagag tataagcagc agttgatggg
    13921 atcccctgtc tacatccaga tcttcaagga agaacgtacg ctcgatcttt acgtcaaaat
    13981 gggcgagcaa tatcaactgc tcgacagtta taaaatctgt aaatattccg gcggcttagg
    14041 gccaaaacag cgtcagggcg atttcaaaag cccggaaggg ttttatagcg tccagcgtaa
    14101 tcagttaaaa ccagacagcc gttactacaa agcgattaat attggattcc ccaatgctta
    14161 tgaccgtgcg catggttatg aagggaaata cctgatgatc cacggcgatt gtgtatccat
    14221 cggctgctac gcaatgacca atcagggtat tgatgagatc ttccagttcg ttactggtgc
    14281 gctggtgttt ggtcagccga gcgtgcaggt gagtatttac ccattccgca tgaccgacgc
    14341 caatatgaag cgccataaat attccaactt taaggacttc tgggagcagc tgaagccggg
    14401 ctacgactac tttgagcaaa cccgtaagcc accgaccgtt tctgtggcca atggccgtta
    14461 cgtggtcagc aagccgttaa gccacgaagt ggtgcagcca caactggcat caaactacac
    14521 gctccccgag gcaaaataag tgccattcgc ctggcataat tttttgccag gtttcattgc
    14581 ccgtcagcgg ctgtgtcgca atcaccgtga ccacatcatt cggtgtggtc tgcgagctga
    14641 agtcgatttc cacatcctga tccagcaacg ttgccacgcc aaacggcgcg cggcgggtga
    14701 tccagtgtaa attagtcgag caatacgcca ttacatagcg cccgtccgaa agcagcatgt
    14761 tgaaaacgcc cttcgcccgc agttcatccg ccagtgaggc gatatattta aacactgccg
    14821 ccatgttgcc cggcgtgcgc gggtaacgct gcgttaattt atgcagcagc cagcaaaagg
    14881 ctttttcgct gtcggtttca ccgaccgggc ggaagttgcc ggtttccagt gatttgtagc
    14941 ccgtcagttg tccgttatgg gcataagtcc agttacgccc ccataactcg cgggtaaatg
    15001 ggtgagtatt ttccagcgcc acctcgcccc gattagcctg gcgaatatga gccaccaccg
    15061 aacaggattt tatcgggtag tcctggacaa gtttggcgat gggggaatta aagctgggtt
    15121 gtggatcttt aaatgtgcga cagcctttac cttcgtaaaa ggtaatgccc cagccatctt
    15181 tatgtggccc ggttccacca ccacgctgta caagtccggt gaaactaaag cagatatcgg
    15241 tagggacgtt ggcgctcatc ccgagcaatt cgcacatggg taacctccac aacagcggga
    15301 gcgcatggac cccggcgaag attgatttaa gaattttcag gtcggatgcg gcgtaaacga
    15361 attattcggc ctacggttcg ggtacagatt tgtaggcatg ataagacgcg tcagcatcgc
    15421 atcaggcatc agcgcacaag tgccggatgc ggcgtaaacg ccttatccgg cctacgccag
    15481 acttacttaa ccatctcttt ttcaattaac tggatcagga tatggatcac tttaatgtga
    15541 atctcctgaa tgcggtcggc ataaccaaag tgcggaacgc gaatttcgat atccgccgtg
    15601 ccagccattt taccgccgtc tttaccggtc agggtgatca ctttcatccc cttctcacgc
    15661 gccgctgcga tcgctttgat cacgtttgca gagttaccgg aggtggagat ccccagcagt
    15721 acatcgcctt cgcgacctac cgcttcaacg tagcgggaga aaatatcatt gaaaccgaaa
    15781 tcattaccga cgcaggaaat atgactaacg tcagaaatag caatcgccgg gtagcccgga
    15841 cggttttcac ggtagcgacc ggtcaactct tcggcaaagt gcatagcgtc gcaatgggaa
    15901 ccgccgttgc cgcaggaaag cactttgcca ccggctttaa agctgtctgc taacaggacc
    15961 gccgcgcgct gaatggcgtg aatattggcg tcatctttta aaaagttagc cagcgtttcc
    16021 gccgcttcgt tcagttcgtt acgaataaga tcctggtaca tgaggatatc cttcagcata
    16081 aatgtaatag acaaaatgca gtgtaccgga taccgccaaa agcgagaagt acgggcaggt
    16141 gctatgacca ggactttttg acctgaagtg cggataaaaa cagcaacaat gtgagctttg
    16201 ttgtaattat attgtaaaca cattgctaaa tgtttttaca tccactacaa ccatatcatc
    16261 acaagtggtc agacctccta caagtaaggg gcttttcgtt atgatgattt tgagtattct
    16321 cgctacggtt gtcctgctcg gcgcgttgtt ctatcaccgc gtgagcttat ttatcagcag
    16381 tctgattttg ctcgcctaga cagccgccct cggcgttgct ggtctgtggt cggcgtgggt
    16441 actggtgcct ctgaccatta tcctcgtgcc atttaacttt gcgcctatgc gtaagtcgat
    16501 gatttccgcg ccggtatttc gcggtttccg taaggtgatg ccgccgatgt cgcgcactga
    16561 gaaagaagcg attgatgcgg gcaccacctg gtgggagggc gacttgttcc agggcaagcc
    16621 ggactggaaa aagctgcata actatccgca gccgcgcctg accgctgaag agcaagcgtt
    16681 tctcgacggc ccggtagaag aagcctgccg gatggcgaat gatttccaga ttactcatga
    16741 gctggcggat ctgccgccgg agttgtgggc gtaccttaaa gagcatcgct tcttcgcgat
    16801 gattatcaaa aaagagtacg gcggactgga gttctcagct tatgcccagt ctcgtgtgct
    16861 gcaaaaactc tctggcgtga gcgggatcct ggcgattacc gtcggcgtgc caaactcatt
    16921 aggcccgggc gaactgttgc aacattacgg cactgacgag cagaaagatc actatctgcc
    16981 gcgtctggcg cgtggtcagg agatcccctg ctttgcactg accagcccgg aagcgggttc
    17041 cgatgcgggc gcgattccgg acaccgggat tgtctgcatg ggcgaatggc agggccagca
    17101 ggtgctgggg atgcgtctga cctggaacaa acgctacatt acgctggcac cgattgcgac
    17161 cgtgcttggg ctggcgttta aactctccga cccggaaaaa ttactcggcg gtgcagaaga
    17221 tttaggcatt acctgtgcgc tgatcccaac caccacgccg ggcgtggaaa ttggtcgtcg
    17281 ccacttcccg ctgaacgtac cgttccagaa cggaccgacg cgcggtaaag atgtcttcgt
    17341 gccgatcgat tacatcatcg gcgggccgaa aatggccggg caaggctggc ggatgctggt
    17401 ggagtgcctc tcggtaggcc gcggcatcac cctgccttcc aactcaaccg gcggcgtgaa
    17461 atcggtagcg ctggcaacgg gcgcgtattc tcacattcgc cgtcagttca aaatctctat
    17521 cggtaagatg gaagggattg aagagccgct ggcgcgtatt gccgggaacg cttatgtaat
    17581 ggatgctgcg gcatcgctga ttacctacgg cattatgctc ggcgaaaaac ctgccgtgct
    17641 gtcggctatc gttaagtatc actgtaccca ccgcgggcag cagtcgatta ttgatgcgat
    17701 ggatattacc ggcggtaaag gcattatgct cgggcaaagc aacttcctgg cgcgtgctta
    17761 ccagggcgca ccgattgcca tcaccgttga aggggctaac attctgaccc gcagcatgat
    17821 gatcttcgga caaggagcga ttcgttgcca tccgtacgtg ctggaagaga tggaagcggc
    17881 gaagaacaat gacgtcaacg cgttcgataa actgttgttc aaacatatcg gtcacgtcgg
    17941 tagcaacaaa gttcgcagct tctggctggg cctgacgcgc ggtttaacca gcagcacgcc
    18001 aaccggcgat gccactaaac gctactatca gcacctgaac cgcctgagcg ccaacctcgc
    18061 cctgctttct gatgtctcga tggcactgct gggcggcagc ctgaaacgtc gtgagcgtat
    18121 ctcggcacgt ctgggggata ttttaagcca gctttacctc gcctctgccg ttctgaagcg
    18181 ttatgacgac gaaggccgta atgaagccga tctgccgctg gtgcactggg gcgtacagga
    18241 tgcgctgtat caggcagaac aggcaatgga caatttactg caaaacttcc cgaaccgcgt
    18301 ggttgccggg ctgctgaatg tggtgatctt cccgaccgga cgtcattatc tggcaccgtc
    18361 tgacaagctg gatcataaag tggcgaagat tttacaagtg ccgaacgcca ctcgttcacg
    18421 tattggtcgc ggtcagtacc tgacgccaag cgagcataac ccggttggtt tactggaaga
    18481 ggcgttggtg gatgtgattg ccgccgaccc gattcatcag cggatctgta aagagctggg
    18541 taaaaatctg ccatttaccc gtctggatga actggcgcac aacgcgctga cgaaggggct
    18601 gattgataaa gatgaagccg ctattctggt gaaagcggaa gaaagccgtc tgcgcagtat
    18661 taacgttgat gactttgatc cggaagagct ggcgacgaag ccggtaaagt tgccggagaa
    18721 agtgcggaaa gttgaagccg cgtaattgaa taacggagcc gaaaggctcc gtttctttat
    18781 ccgctaatta tttaaaatta aagccatccg gatggttttc caggctgccg gtcaacgccg
    18841 cgaacagcac cgttttacca tcaatcgaaa gcgcatcgtt cacattcagc caggtgagtt
    18901 tctcttgcga cgttttctca tcaatagccg agaacagccc cgtcatctga ttagatttct
    18961 cggaccacat cacagcgata cgttgcgagc cacagtcatg cggtttgcac gcgctcatca
    19021 cctgatacgt ctcatctccc aacgttacgg tttgtgcggg agtataagta ccgcctttca
    19081 tcacccatgc aggtagctta tgcccttgca ccatctgatt aaacgcagct ttggtggttt
    19141 cgccctttgc aaggctgcta atggttaaat catcctgcgc cattgcactg gtggcgatga
    19201 ccagtgcggc gactgtcgtt attgccttaa acatcattcc tcccgagctt atcctgccca
    19261 tgttattaac ctcctaaagc ctggcgataa cacgataatt tgcaaatcag atatttccaa
    19321 tatcagcatt tgttatcaat ttattctgcg ctaaggcaac tgcctgataa ggtgttaaag
    19381 ttataaaacc cttcgcataa aggagttgta caccgtgcct agtttgaaga ttacgctttt
    19441 gcagcaaccg ttggtgtggg tggatggtcc tgccaacctg cgccattttg atcgtcaact
    19501 ggaaggtatt accgggcgcg atgtgatcgt tctaccggag atgtttacca gcggctttgc
    19561 catggaagcg gcagcttcgt cgcttgcaca agatgacgta gtgaactgga tgacagctaa
    19621 ggcgcagcag tgcaatgcgc tgattgcagg cagtgttgca ttacaaacgg agtctggttc
    19681 ggttaaccgc tttttgctgg ttgagccggg cggcacggta catttttatg ataagcgtca
    19741 tctgttccgc atggcagatg agcatctgca ttataaagcg ggcaatgcgc gagtgattgt
    19801 ggaatggcgc ggctggcgta ttttgccgct ggtgtgctac gacttacgtt ttccagtgtg
    19861 gtcgcgcaat ctcaacgatt atgacctcgc cctgtacgtc gccaactggc cagctccgcg
    19921 ctctctgcac tggcaggcat tgctgacggc ccgcgcgatt gagaatcagg cgtatgtggc
    19981 gggatgcaat cgcgtcggca gcgatggcaa cggctgccat tatcgcggtg acagccgggt
    20041 gattaatccg caaggcgaga ttatcgctac cgccgacgcg cataaggcaa cgcgtattga
    20101 tgcggagctg tcgatggtgg cattgcggga atatagagaa aagtttccgg catggcggga
    20161 tgcggatgag tttaggttgt ggtgagtggg ggttgtgttt agggccgggg gctgggctag
    20221 attacgaaag cccctaactt aaacacaata ttacgccaga agtcaccatt tccaaaacac
    20281 agcgtcgctg taccgtgggt tatctgccca accgcagcga cacgccggtg atcaaaatca
    20341 gcgccaaatg gctgagaaaa gcgggatttg agatcggcac gggcgttacc gtgaagatct
    20401 cggagggatg tttgatcctg cttgggataa caacgaagta caggaactgc gcagggaact
    20461 ttatcaggtt aaacagccag ttaagggga
//
LOCUS       DAJTQY010000073        20435 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.073, whole genome shotgun sequence.
ACCESSION   DAJTQY010000073 DAJTQY010000000
VERSION     DAJTQY010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20435
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.073"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            167..508
                     /gene="raiA"
                     /locus_tag="OO349_002730"
     CDS             167..508
                     /gene="raiA"
                     /locus_tag="OO349_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HCR5867737.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    611..736
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            612..659
                     /gene="pheL"
                     /locus_tag="OO349_002731"
     CDS             612..659
                     /gene="pheL"
                     /locus_tag="OO349_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phe operon leader peptide"
                     /protein_id="HCR5867738.1"
                     /translation="MKHIPFFFAFFFTFP"
     gene            758..1918
                     /gene="pheA"
                     /locus_tag="OO349_002732"
     CDS             758..1918
                     /gene="pheA"
                     /locus_tag="OO349_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HCR5867739.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(1961..3082)
                     /gene="tyrA"
                     /locus_tag="OO349_002733"
     CDS             complement(1961..3082)
                     /gene="tyrA"
                     /locus_tag="OO349_002733"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HCR5867740.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGKVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIHVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3093..4163)
                     /gene="aroF"
                     /locus_tag="OO349_002734"
     CDS             complement(3093..4163)
                     /gene="aroF"
                     /locus_tag="OO349_002734"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HCR5867741.1"
                     /translation="MHKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHCFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4373..4738
                     /locus_tag="OO349_002735"
     CDS             4373..4738
                     /locus_tag="OO349_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HCR5867742.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKRADENASTIRLN"
     gene            4888..5406
                     /locus_tag="OO349_002736"
     CDS             4888..5406
                     /locus_tag="OO349_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HCR5867743.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDASSRSGVKVNP
                     DVLMLARKKNDG"
     gene            5396..6621
                     /gene="dgcN"
                     /locus_tag="OO349_002737"
                     /pseudo
     CDS             5396..6621
                     /gene="dgcN"
                     /locus_tag="OO349_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
     gene            6637..7119
                     /locus_tag="OO349_002738"
     CDS             6637..7119
                     /locus_tag="OO349_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HCR5867744.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(7196..7543)
                     /gene="rplS"
                     /locus_tag="OO349_002739"
     CDS             complement(7196..7543)
                     /gene="rplS"
                     /locus_tag="OO349_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HCR5867745.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(7585..8352)
                     /gene="trmD"
                     /locus_tag="OO349_002740"
     CDS             complement(7585..8352)
                     /gene="trmD"
                     /locus_tag="OO349_002740"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HCR5867746.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(8383..8931)
                     /gene="rimM"
                     /locus_tag="OO349_002741"
     CDS             complement(8383..8931)
                     /gene="rimM"
                     /locus_tag="OO349_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HCR5867747.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(8950..9198)
                     /gene="rpsP"
                     /locus_tag="OO349_002742"
     CDS             complement(8950..9198)
                     /gene="rpsP"
                     /locus_tag="OO349_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HCR5867748.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(9335..10696)
                     /gene="ffh"
                     /locus_tag="OO349_002743"
     CDS             complement(9335..10696)
                     /gene="ffh"
                     /locus_tag="OO349_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HCR5867749.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGNFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            10863..11654
                     /locus_tag="OO349_002744"
     CDS             10863..11654
                     /locus_tag="OO349_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HCR5867750.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLVICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLMHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            11675..12961
                     /locus_tag="OO349_002745"
     CDS             11675..12961
                     /locus_tag="OO349_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCR5867751.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGKYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(13016..13609)
                     /gene="grpE"
                     /locus_tag="OO349_002746"
     CDS             complement(13016..13609)
                     /gene="grpE"
                     /locus_tag="OO349_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HCR5867752.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            13732..14610
                     /gene="nadK"
                     /locus_tag="OO349_002747"
     CDS             13732..14610
                     /gene="nadK"
                     /locus_tag="OO349_002747"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HCR5867753.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            14696..16357
                     /gene="recN"
                     /locus_tag="OO349_002748"
     CDS             14696..16357
                     /gene="recN"
                     /locus_tag="OO349_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HCR5867754.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKYDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            16506..16847
                     /gene="bamE"
                     /locus_tag="OO349_002749"
     CDS             16506..16847
                     /gene="bamE"
                     /locus_tag="OO349_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HCR5867755.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(16909..17199)
                     /locus_tag="OO349_002750"
     CDS             complement(16909..17199)
                     /locus_tag="OO349_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HCR5867756.1"
                     /translation="MLGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(17189..17626)
                     /gene="ratA"
                     /locus_tag="OO349_002751"
     CDS             complement(17189..17626)
                     /gene="ratA"
                     /locus_tag="OO349_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HCR5867757.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            17797..18279
                     /gene="smpB"
                     /locus_tag="OO349_002752"
     CDS             17797..18279
                     /gene="smpB"
                     /locus_tag="OO349_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HCR5867758.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            18494..18856
                     /gene="ssrA"
                     /locus_tag="OO349_002753"
     tmRNA           18494..18856
                     /gene="ssrA"
                     /locus_tag="OO349_002753"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            19061..20302
                     /locus_tag="OO349_002754"
     CDS             19061..20302
                     /locus_tag="OO349_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR5867759.1"
                     /translation="MARQTKPLSVKEIESAKPKEADYVLYDGDGLELLIKSSGSKIWQ
                     FRYIRPVTKTRAKKSIGPYPSVTLADARNYRAESRSLLAKQIEPQEHQQEQLRSSLEA
                     KTNTFQLVAERWWNVKKASVTEDYAEDIWRSLERDVFPAIGDVSVTDIKAHTLVQAVQ
                     PVQARGALETVRRLCQRINEVMIYAQNTGLIDAVPSVNIGKAFEKPQKKNMPSIRPDQ
                     LSQLMQTMRTASISLSTRCLFMWQLLTITRPAEAAEARWEEVDIEAQEWKIPAARMKM
                     NRDHTVPLSDEAIAVLEMMKPLSGNREFIFPSRIKPNQPMNSQTVNASLKRAGFGGVL
                     VSHGLRSIASTALNEQGFPPDAIEAALAHVDKNEVRRAYNRSDYLEQRRPMMQWWANF
                     VMAADRGSMIEGGIKGMKLVG"
BASE COUNT         5282 a         5342 c         4797 g         5014 t
ORIGIN      
        1 ctcgctttga gatattcctc aagtaaaaaa acacctcttc ctgcgatttc tcacaaaaaa
       61 gattcgttga caaaaagtga caaaattatg agatttccat cacacatttt gacatcagga
      121 acggtatgct gaattcacca agacgggaag acaagaggta aaatttatga caatgaacat
      181 taccagcaaa caaatggaaa ttactccggc catccgccaa catgtcgcag accgtctcgc
      241 caaactggaa aaatggcaaa cacatctgat taatccacat atcattctgt ccaaagagcc
      301 acaagggttt gttgctgacg ccacaatcaa tacacctaac ggcgttctgg ttgccagtgg
      361 taaacatgaa gatatgtaca ccgcaattaa cgaattgatc aacaagctgg aacggcagct
      421 caataaactg cagcacaaag gcgaagcacg tcgtgccgca acatcggtga aagacgccaa
      481 cttcgtcgaa gaagttgaag aagagtagtc ctttatattg agtgtatcgc caacgcgcct
      541 tcgggcgcgt tttttgttga cagcgtgaaa acagtacggg tactgtacta aagtcactta
      601 aggaaacaaa catgaaacac ataccgtttt tcttcgcatt cttttttacc ttcccctgaa
      661 tgggaggcgt ttcgtcgtgt gaaacagaat gcgaagacga acaataaggc ctcccaaatc
      721 ggggggcctt ttttattgat aacaaaaagg caacactatg acatcggaaa acccgttact
      781 ggcgctgcga gagaaaatca gcgcgctgga tgaaaaatta ttagcgttac tggcagaacg
      841 gcgcgaactg gccgtcgagg tgggaaaagc caaactgctc tcgcatcgcc cggtacgtga
      901 tattgatcgt gaacgcgatt tgctggaaag attaattacg ctcggtaaag cgcaccatct
      961 ggacgcccat tacattactc gcctgttcca gctcatcatt gaagattccg tattaactca
     1021 gcaggctttg ctccaacaac atctcaataa aattaatccg cactcagcac gcatcgcttt
     1081 tctcggcccc aaaggttctt attcccatct tgcggcgcgc cagtatgctg cccgtcactt
     1141 tgagcaattc attgaaagtg gctgcgccaa atttgccgat atttttaatc aggtggaaac
     1201 cggccaggcc gactatgccg tcgtaccgat tgaaaatacc agctccggtg ccataaacga
     1261 cgtttacgat ctgctgcaac ataccagctt gtcgattgtt ggcgagatga cgttaactat
     1321 cgaccattgt ttgttggtct ccggcactac tgatttatcc accatcaata cggtctacag
     1381 ccatccgcag ccattccagc aatgcagcaa attccttaat cgttatccgc actggaagat
     1441 tgaatatacc gaaagtacgt ctgcggcaat ggaaaaggtt gcacaggcaa aatcaccgca
     1501 tgttgctgcg ttgggaagcg aagctggcgg cactttgtac ggtttgcagg tactggagcg
     1561 tattgaagca aatcagcgac aaaacttcac ccgatttgtg gtgttggcgc gtaaagccat
     1621 taacgtgtct gatcaggttc cggcgaaaac cacgttgtta atggcgaccg ggcaacaagc
     1681 cggtgcgttg gttgaagcgt tgctggtact gcgcaaccac aatctgatta tgacccgtct
     1741 agaatcacgc ccgattcacg gtaatccatg ggaagagatg ttctatctgg atattcaggc
     1801 taatcttgaa tcagcggaaa tgcaaaaagc attgaaagag ttaggggaaa tcacccgttc
     1861 aatgaaggta ttgggctgtt acccaagtga gaacgtagtg cctgttgatc caacctgatg
     1921 aaaaggtgcc ggatgatgtg aatcatccgg cactggatta ttactggcga ttgtcattcg
     1981 cctgacgcaa taacacgcgg ctttcactct gaaaacgctg tgcgtaatcg ccgaaccagt
     2041 gctccacctt gcggaaactg tcaataaacg cctgcttatc gccctgctcc agcaactcaa
     2101 tcgcctcgcc gaaacgctta tagtaacgtt tgattaacgc cagattacgc tctgacgaca
     2161 taatgatgtc ggcataaagc tgcggatcct gagcaaacag tcgcccgacc atcgccagct
     2221 caaggcggta aatcggcgaa gagagcgcca gaagttgctc aagctgaaca ttttcttctg
     2281 ccaggtgcag cccgtaagca aaagtagcaa agtggcgcag agcctgaata aacgccatat
     2341 tctgatcgtg ctcgacagcg ctaatacgat gcaaccgagc gccccagaca tgaatttgct
     2401 ccagaaacca ttggtatgct tccggcttac gtccatcaca ccagaccaca acttgctttg
     2461 ccaggctacc gctgtccggg ccgaacatcg ggtgtaaccc cagtaccggg ccatcgtgcg
     2521 ccgccagcat ggcctgtaat ggtccatttt tcactgatgc cagatcaacc agaatacaat
     2581 ctttcggtaa aggcggtaat ttgccgataa cttgctcagt aacgtggatt ggcacactaa
     2641 caatcaccat tccggcatcg gcaacaatat cagccgctcg atcccagtca tgttgctcca
     2701 gaatccgcac ctgataaccc gatagtgtca gcatcttctc gaacaggcgt cccatctgac
     2761 cgccaccgcc gacgataacc accggacgca gtgacggaca aagtgtttta aatcctttgt
     2821 cgttttcact ggagtaagat tcacgcatca cccgacgcaa aacatcctca atcagatctg
     2881 gcggtacacc cagagcttcc gcctctgcac gacgcgaggc caacatagat gcctcgcgct
     2941 ccggaacata aataggcagt ccaaagcggc ttttcacctt gcccacttca gcaaccagtt
     3001 ccagacgctt cgctaataaa ttcagcagcg ctttatcgac ttcatcaatt tgatcgcgta
     3061 atgcggtcaa ttcagcaacc ataataaacc tcttaagcca cgcgagccgt cagctgcccg
     3121 ttcagatcct gatgaatttc acgcagcaag gcatcggtca tttcccagct aatgcaggca
     3181 tcggttacgg atacaccgta tttcatttca ctgcgcggtt gctcagaaga ctgattgccc
     3241 tcgtggatat tactttcgat catcagacca ataattgagc gattaccatc tttgatttga
     3301 gcaaccacag attctgccac cgcaggctga cggcgataat ctttattgga attaccgtgg
     3361 ctgcaatcta ccatcagaga cgggcgcagt cccgcctgtt ccatctcttt ttcacattgc
     3421 gcaacatccg cagggctata gttcggcgct ttaccaccgc gcaggatcac atgcccgtcc
     3481 ggattcccct gagtttgtag caacgcaacc tgccctgcct ggttaatgcc aacaaaacag
     3541 tgcggctggg cggcggcgcg catagcgtta attgctgttg ccagactgcc gtcggtgccg
     3601 tttttaaaac caaccggcat ggaaagcccg gaggccattt cacggtgagt ttgcgattcc
     3661 gttgtacgag caccaattgc tgaccagcta aacagatcgc ccaggtattg cgggctattc
     3721 ggatctaacg cttccgtcgc cagtggcagt cccatattca ccagctcaag caacaattta
     3781 cgcgcgatct gcagcccggc ttctacatca aaagagccat ccatatgggg atcgttaatt
     3841 aaccctttcc agccgacagt ggtacggggt ttttcaaaat agacgcgcat taccagatag
     3901 aggctatcgc tgacctctgc ggcaagagct ttaaatcgac gagcatattc cagagcagtt
     3961 tccggatcat gaatggaaca aggaccacat actaccagca gacgaggatc gcgcccggcg
     4021 ataatatctg aaatggtttt acgcgagtca gcaatctggg cttcttgttg caggctcaat
     4081 ggaaaagcgg ccttcagttg ttccggagtc attaaaacct gttcgtcggt aatatgtacg
     4141 ttattcagcg cgtctttatg catgatggcg atcctgttta tgctcgtttg cgatagttgc
     4201 tcctcagcga ggatgacgta acgataacac ataaagtaaa gttttcaatc catatttcgt
     4261 acatttttat ttacacagac aatttagtcg cgctttcaac ccttgcttct gtatagataa
     4321 atttacattc cctttaaaaa caatccgcta tgctttgaaa aaggagaaag aaatgatgaa
     4381 aaagtttatc gcccccttgt tggctttact ggttagcgga tgtcagattg atccttatac
     4441 tcacgcgcca accttgacca gcaccgactg gtatgatgtc ggtatggaag atgcgatatc
     4501 gggcagcgcc ataaaagatg acgatgcatt tagcgattca caggcggatc gcggtctata
     4561 ccttaaagga tatgccgaag ggcaaaagaa aacttgccag accgatttta cctatgcccg
     4621 gggactttcc ggtaaaagct ttcctgccag ctgcaacaat gttgaaaatg ccagccaact
     4681 gcatgaagta tggcaaaaaa gggctgatga aaatgccagc acaatacgat taaattaatt
     4741 aaaaccccac aaataaatta aacataagat tttactcatt gtgttttatt tctcacattg
     4801 atgacggtcg catgaaaaaa tgatatttta atgagcatcc tgaaaaaata tcgatgcatt
     4861 tcgagcgaag atggtgggga tccctgaatg cgtttttctc accgactgtt cttacttctg
     4921 attctcctgc tgacgggagc accaatactc gctcaagagc cttctgacgt tgcgaaaaac
     4981 gtgcgtatga tggtttccgg tattgtcagt tacacccgct ggcctgcgct atcagggcca
     5041 ccaaagttat gtatcttttc atcttcacgt tttagtaccg ccctacaaga gaatgccgcg
     5101 acatctttgc cttatctgcc tgtcatcatt cacactcaac aagaggcgat gatttcaggc
     5161 tgcaatggtt tttattttgg caacgagtca ccgacattcc agatggaatt aaccgaacaa
     5221 tatccgtcaa aggcgttatt attaattgcc gaacaaaata ccgagtgcat tattggtagc
     5281 gccttttgtc tgatcatcca caataatgac gtcagatttg ccgtaaacct ggatgcctca
     5341 tcgcgtagcg gtgtaaaggt caacccggat gtcttaatgc tcgcacggaa gaaaaatgat
     5401 ggataacgat aattctctta ataagcgccc cacgtttaaa agagcattac gcaacatcag
     5461 tatgaccagc atatttatca ctatgatgct gatctggttg ctgctttccg tgacctcggt
     5521 gctgaccctg aaacagtacg cgcaaaaaaa cctggcactg acagcagcaa caatgactta
     5581 cagtctggaa gcagctgtcg tttttgccga tggccctgca gcaactgaaa cactggcagc
     5641 gctgggccag caagggcatt ttcaactgca gaagtacgtg ataagcagca aaatattctg
     5701 gcatcctggc attacccccg taaggatcca ggcgatactt tcagcaattt cataagccac
     5761 tggctcttcc ctgcccccat cattcagccg attcgtcaca atggtgaaac cattggcgaa
     5821 gtacgcttaa ccgctcgcga cagttcaatc agccatttta tctggttttc gctcgccgta
     5881 ctgaccggtt gtattctgct ggcatcaggc atcgcaatta ccctcacccg ccatttgcac
     5941 aatggcctgg tggaagcact gaaaaatatc accgatgtcg tacatgatgt gcgttccaac
     6001 cgcaattttt cccgacgagt ttcggaagaa cgtatcgctg agtttcaccg cttcgctctc
     6061 gacttcaaca gtctgctgga tgaaatggaa gagtggcagc ttcgtttaca ggccaaaaat
     6121 gcgcagcttc tgcgtaccgc gctacatgac ccattaaccg ggctggctaa ccgcgcagcg
     6181 tttcgtagcg gcatcaatac gttgatgaac aattccgatg cccgaaaaac gtcggcgtta
     6241 ctatttcttg atggcgataa tttcaaatat atcaatgata cctggggtca tgcgacgggc
     6301 gatagggtct tgattgaaat cgcaaaacgg ttagctgaat ttggcgggct gcgacataaa
     6361 gcataccgcc tgggcggcga tgaattcgct atggtgctct atgatgtaca gtcggaatct
     6421 gaagtgcagc agatatgctc agcactgaca caaatcttta atctcccgtt tgatcttcat
     6481 aatggccatc agaccaccat gacattaagc attggttacg cgatgaccat tgagcacgcc
     6541 tctgcggaaa aattacaaga gcttgccgat cacaatatgt atcaggccaa acaccagcgt
     6601 gccgaaaagc tggtgagata acaaggatat atcgatatga taaagcacct ggtagcaccc
     6661 ctgattttca cctcactaat actgactggc tgccagtccc ctcagggaaa gtttactcct
     6721 gagcaagtcg ccgctatgca atcttatgga tttactgaat ccgccggcga ctggtcgctg
     6781 ggcttatcag atgccattct gttcgcaaaa aatgactaca aattgctccc ggaaagccag
     6841 caacagatcc aaaccatggc agccaaattg gcctcgacag ggctaacaca tgcccgtatg
     6901 gatggacaca ccgataacta tggtgaagac agttacaacg aaggcttatc attgaaacgg
     6961 gcgaatgtcg tggccgatgc atgggctatt ggtggacaaa ttccacgcag caatctcacc
     7021 acacagggtt taggaaaaaa atatcccata gccagtaaca agaccgccca gggccgcgcc
     7081 gagaaccgcc gcgtcgcagt ggtgattaca accccttaaa acacgagcaa acagacaaaa
     7141 aaagggccag ccaattggcc agcccttctt aacaggatgt cgcttaagcg aaatcttagt
     7201 taagacgctc tttgatacga gcagccttac cagtacgctc acgcaggtag tacagtttag
     7261 ctttacgaac agcaccacga cgtttgacag aaatgctgtc aactaccgga gagtgagtct
     7321 ggaagacacg ctcaacgcct tcgccgttgg aaattttacg aacagtgaat gcagagtgca
     7381 gaccgcggtt acgaatagcg ataaccacgc cctcgaatgc ctgcagacgt tttttggaac
     7441 cttcaacaac ccatactttc acttccacgg tatcacccgg acggaaggaa ggtacgtcct
     7501 gcttcatctg ctcttgttca agttgcttaa taatgttgct cataatttaa tctcttatcc
     7561 tgggtaaact gatctctcgg gggcttacgc catcccatca tgtttatgtt gctgttgtgc
     7621 gtgttccgtt ttgaactccg ccagcaacct tgcttgctct tcagtcagag ccaggttttc
     7681 cagaagttca ggtcttctaa gccaggtacg gcccagcgac tgtttcaaac gccagcgacg
     7741 tatctcggca tggttgcccg acagtaacac tggcggaact tccatccctt ctaacacctc
     7801 aggccgcgta tagtgcgggc aatccagcaa tccttcagca aaggaatctt ccgttgccga
     7861 ggcttcatgt cccagtactc ccggaataaa ccgggaaacg gagtcaatca gcgtcattgc
     7921 tggtaactca ccaccactga gaacgtaatc gccgattgac cattcttcgt caatttcggt
     7981 ttggatcacg cgctcatcta taccttcgta gcgaccgcac accagaatca atttttgatt
     8041 cgttgccagt tcgctgacgc ccgcttgatc aagcttgcgt ccctgtggtg acagataaat
     8101 cacctttgcg ccttcacccg ccgcggcttt tgctgcatga atggcgtccc gcaagggttg
     8161 caccatcatt aacatccccg gtccgccgcc gtaaggacga tcgtccacgg tacggtgccg
     8221 gtcatgcgtg aagtcgcgag gactccaact ctggatgctc agcaggccat ttttaactgc
     8281 ccggccagtt accccgtaat cggtaattgc gcggaacatt tcaggaaaca ggctaattat
     8341 gccaatccac atagcgccgc cttttaccgt ttatccggtg gtttaaaaac caggatccca
     8401 atctacttcg attgaacgag tagtgagatc gactttcttg ataacctgcc catcgaggaa
     8461 cggtacgaga cgttccttga taccaaacgc atctttcagg tttgccttaa tgacgagaac
     8521 gtcattagat ccggtttcca tcatatcaac gactttaccg agatcgtagc cttcagtggt
     8581 tactacctgg cagcccatca ggtctttcca gtagtagtcc ccctcttcaa gctgaggcag
     8641 ctgcgatgaa tccacgacaa tttcacaatt cgtcagcagg ttcgccgcat cacgatcgtc
     8701 aacgcctttc agcttgatga tcatgtcctg attgtggtgc ttccagcttt ccagctggac
     8761 ttgctgccac tgacccgcct tctggataaa ccagggctga tagtcaaaaa tgctttcggc
     8821 gtcttcggtg gaagaaaaca ctctgagcca cccacgaata ccgtaagacg aacccatttt
     8881 tcccaaaacg atgggatcaa caggtgcttg cgcggtgagt tgtttgctca tcatgaccac
     8941 cgtgacagat taagctgctt tgtttacttc tttgatcagc gcagcaacgc gatcagaaat
     9001 agttgcgccc tggccaaccc agtgagcgat gcgatccaga tccaggcgag tgccttcttc
     9061 tttttcgcta gcgattgggt tgaagaaacc aacgcgctcg atgaagcgac cgttgcgtgc
     9121 attacggctg tcagcgacaa caacctggta gaacggacgc tttttagcgc cgtgacgtgc
     9181 taaacgaata gttaccataa catcctcttg tgtgaataaa acaaccggac cccatcgagg
     9241 aacggagtcc ggtgtcatat taaaagcccg aaaattttac tgatttctgg agaaaatgca
     9301 atcagcagtt aataactctg tcgcataagg tcatttagcg accaggaaat cccggcggca
     9361 tcattccctt catgcttctc atcatcttcg ccattccgcc tttcttcatt ttcttcatca
     9421 tgcgctgcat gtcgtcgaac tgcttcagaa ggcggttaac gtcctgtacc tgcatcccgc
     9481 aaccggcggc gatacgacgc ttacgcgaac ctttgatgat ttctggctta gcgcgctctt
     9541 tcatcgtcat tgagttgatg atggcttcca tacgcaccag cactttatcg tccatctgcg
     9601 acttgacgtt atccgggatc tgccccatgc ccggcagttt gcccatcaag ctggccatgc
     9661 cgcccatatt tttcatctgg cgcagttgct caagaaagtc gttgagatcg aagccgtcgc
     9721 ctttttttag cttgctggct aatttctctg cctgcgcgcg gtcaactttg ctttcgatat
     9781 cttcgatcag cgacagtacg tcgcccatgc cgagaatacg cgacgcgatg cggtccggat
     9841 ggaacggctc cagcgcctca gttttctcgc caacaccgag gaacttgatc ggtttgccag
     9901 tgatgtgacg aatagagagc gccgcaccgc cgcgggcatc gccgtccact ttggtcaaca
     9961 ctacgccggt aagcggtaac gcttcattga atgcttttgc cgtattggcc gcatcctgac
    10021 cggtcatggc gtcaaccaca aacagggttt caaccgggtt aatcgacgca tggacttgtt
    10081 tgatctcgtc catcatcgct tcgtcaacgt gcagacgacc agcggtatcc accagcagca
    10141 cgtcgtagaa tttcagtttg gcttctttca gcgccgcgtt aacaatatca accggcttct
    10201 gaccaacatc agaagggaag aaatcaacgc ccacctgctc tgccagcgtc tcaagctgtt
    10261 tgattgccgc cgggcgatag acgtcagcag aaaccaccag cactttcttc ttgtgcttct
    10321 cgcgcaggaa attaccgagt ttaccaacgc tggttgtttt accggcacct tgcaggcctg
    10381 ccatcagcac gaccgcaggc ggttgcgcag ccagattcag ggtctggttc tcttcgccca
    10441 tcgccgcaac cagttcgtta cggacgattt tgacgaactc ctgccccggc gtcaggctct
    10501 tattaacttc atgaccaacc gctttctctt ttacgcgatt gataaactca cgcactaccg
    10561 gcagagcgac gtccgcctcc agcagcgcca tgcgcacttc gcgcagcgta tcttttacgt
    10621 tgtcttcagt gaggcgtcca cggccactga tattgcgcag cgtgcgcgac aaacgatcgg
    10681 ttaaattatc aaacattgtc tctcgcctgg ggtggaaacg gttggccgca atcgcgacac
    10741 atcatcagta ttttgccgca gtataacatg aaggcgtctt tgttgttatg caacggttgg
    10801 agcagcgttc acctgacgct atactgcttc tctttcttat tgctcaaact gtcgacatta
    10861 ctatgcccgt ttttgctctg ctcgcgcttg tcgcctactc cgtcagtctt gcgctgattg
    10921 ttcccggtct gctgcaaaaa aacggcggct ggcggcgcat ggctattatt tctgcggtca
    10981 ttgcgctggt ctgccacgca atcgctctgg aagcccgcat cctgcccgac ggtgatagcg
    11041 gacaaaacct cagcctgctg aacgttggtt cattggtcag tttggtgatc tgtacggtaa
    11101 tgaccattgt ggcttctcgc aatcgtggct ggttgctgct acccattgtc tatgcctttg
    11161 cgcttatcaa cctggcgctg gcgaccttca tgcccaatga atacatcacc catctggaag
    11221 caacgcctgg gatgctgatg cacattggct tatcgctctt ttcctatgcc acgctaatta
    11281 tcgccgccct gtacgcgctg caactggcgt ggattgatta ccaactgaag aacaagaagc
    11341 tggcatttaa ccaggaaatg ccgccattga tgagtatcga gcgtaaaatg ttccacatca
    11401 cgcagattgg cgtggtgctg ctaacgctca cgctttgcac tggcctgttc tacatgcaca
    11461 acctatttag catggaaaat atcgacaagg ctgtgctctc tatcgtggcg tggtttgtct
    11521 atattgtgct gctgtgggga cattaccatg aaggatggcg tggacgccgc gtcgtctggt
    11581 ttaacgttgc gggcgcggta attctgacac tggcctactt cggcagccga attgtccagc
    11641 agttaatcag ctaaacccag aaaaggagtt tcccctggaa cacatttcta ctactacgtt
    11701 gatcattatt ctgatcatca tggtggtcat ttcagcctat ttttccgggt ccgaaaccgg
    11761 aatgatgacc ctcaaccgct atcgtcttcg acatatggcg aaacagggta atcgctcggc
    11821 caaacgcgtc gaaaaattgc tgcgtaagcc agaccgcctg ataagcctgg tgttaatcgg
    11881 caataacctg gtcaatattc ttgcctccgc gctcggcact attgtcggga tgcgtttgta
    11941 cggcgatgcg ggcgtggcaa ttgcgactgg tgtgctgact tttgtcgtac tggtttttgc
    12001 tgaggtattg ccgaaaacca ttgccgcgct gtacccggaa aaagtcgctt atccgagtag
    12061 ttttctgctg gctccgctgc aaattttgat gatgccgctg gtctggttgc tgaatgctat
    12121 cacccgtatg ctgatgcgca tgatgggtat caaaaccgat atcgtggtta gcggctcttt
    12181 gagcaaagaa gagttgcgca ctatcgtgca tgaatcgcgc tcacaaattt cccgtcgcaa
    12241 tcaggatatg ctgctgtcgg tgctcgatct ggaaaaaatg accgttgatg acatcatggt
    12301 accgcgcagt gaaattatcg gtattgatat caacgatgac tggaaatcta ttctgcgcca
    12361 actctcccac tcacctcacg ggcgcatcgt gctttaccgt gattcgctgg acgacgccat
    12421 cagtatgctg cgagtacgtg aagcctggcg gttgatgtcg gagaaaaaag agttcaccaa
    12481 agaaaccatg ctgcgcgccg cggacgagat ctattttgtg cctgaaggta cgccgctcag
    12541 cacgcagttg gtaaagtttc agcgcaacaa aaagaaagtc ggcctggtcg tcaacgagta
    12601 tggagacatt caggggctgg tgacggttga agatattctg gaagagattg tcggcgattt
    12661 cactacgtcg atgtcgccaa cacttgccga agaggtcacg ccgcaaaacg acggttcggt
    12721 gattatcgat ggcaccgcca acgtgcggga aatcaacaaa gcctttaact ggcatctacc
    12781 ggaagatgat gcccgcacgg ttaatggcgt cattcttgag gcactggaag agatccctgt
    12841 cgcaggcacc cgcgtgcgta ttggcaagta cgatatcgat attctcgacg tacaggacaa
    12901 tatgattaag caggtaaaag tttttcctgt gaaaccgctg cgcgagagtg tggcggagta
    12961 acgaaaacgg ccctgcattc gcatgcaggg ccgtgaatta ttacgaaaac agaaattacg
    13021 cttttgcttt cgctacagtt accatcgccg cacgaatcgt acgaccattc agcgtataac
    13081 ccttctgcat aatgcccagt acgttacctg gcgcaacgtc atcagattcc accattgcga
    13141 tagcctgatg cacattcggg tccagcggca cgttagtttc ggcgatcact tcaacaccaa
    13201 acttacgcac aacatccagc atcgacttca gcgtcagctc aatgccttca accatcgcag
    13261 acatatccgg gttagcttta tcagccactt ccagcgcacg atccaggcta tcaatcaccg
    13321 gcagcaattc gttgatgaat ttctccagcg cgaatttgtg ggctttttca atatccagtt
    13381 cagtacgacg acgcaggttt tccatttcgg cttttacgcg caaaataccg tcacgttcac
    13441 gggtctgggc ttcagccagc tgagcttcga gattcgcaat tttttcatcg cgcggatcca
    13501 cctgctcagc agaagcttct ggctcaactg cctcaatctc ttcgtgctga tccatgataa
    13561 tttcttccgg ggcttgcccc tcaggcgttt tctgttcttt actactcatg aatttctccg
    13621 cgtttttttc gcattcatct cgctaacttc gcttattatg gggatcagtt tcagggtttc
    13681 aagggaagca ctcacattgt catcaatctt cgcaacaagg acctcggaaa aatgaataat
    13741 catttcaagt gtattggcat tgtgggacac ccacggcacc ctactgcact gacaacacat
    13801 gaaatgctct accgctggct gtgcacaaaa ggttacgagg tcatcgttga gcaacaaatc
    13861 gctcacgaac tgcaactgaa gaatgtgaaa actggcacgc tcgcggagat tgggcaactg
    13921 gctgatctcg cggtagtcgt tggtggcgac ggtaatatgc tgggcgcggc gcgcacgctc
    13981 gcccgttacg atattaaagt tattggaatc aaccgtggca acctgggttt cctgactgac
    14041 cttgaccccg ataacgccca gcaacaatta gccgatgtgc tggaaggtca ctacatcagc
    14101 gagaaacgtt ttttgctgga agcgcaagtc tgtcagcaag attgccaaaa acgcatcagc
    14161 accgcgatta acgaagtggt acttcatcct ggcaaagtgg cgcatatgat agagttcgaa
    14221 gtgtatatcg acgagatctt tgcgttttct cagcgatctg atgggctaat tatttcgacg
    14281 ccaacaggct ccaccgccta ttccctctct gcgggcggtc ctattctgac cccctctctg
    14341 gatgcgatta ccctggtgcc catgttcccg catacgttgt cagcacgacc actggtcata
    14401 aacagcagca gcacgatccg tctgcgtttt tcgcatcgcc gtaacgacct ggaaatcagt
    14461 tgcgacagcc agatagcact gccgattcag gaaggtgaag atgtcctgat tcgtcgctgt
    14521 gattaccatc tgaatctgat tcatccgaaa gattacagtt atttcaatac attaagcacc
    14581 aagctcggct ggtcaaaaaa attattctaa ttttacgcca gcctctttac tgtatataaa
    14641 accagtttat actgtacaca ataacagtaa tggtttttca tacaggaaaa cgactatgtt
    14701 ggcacaactg accatcagca actttgctat cgttcgtgag cttgagattg attttcatag
    14761 cggcatgacc gtaataactg gcgagactgg tgcgggtaaa tctattgcaa tagatgccct
    14821 cggtctttgt ctcggtggtc gcgctgaagc cgacatggtg cgtaccggcg ctgctcgcgc
    14881 tgacctgtgc gcccgttttt ctctgaaaga tacgccagcg gccctgcgct ggctggaaga
    14941 aaaccagctt gaagacgggc atgaatgttt gcttcgtcgc gtgatcagca gtgatggtcg
    15001 ctcccgtggt ttcatcaacg gtacagctgt tcctctgtca caactgcgcg aactgggtca
    15061 gttgctgatt cagatccatg gtcagcacgc tcatcaatta ctcaccaaac ctgagcacca
    15121 aaaattcctg cttgatggct atgccaatga aacctctcta cttcaggaaa tgaccgcacg
    15181 ttatcagttg tggcatcaaa gctgccgtga cctcgcgcat catcaacagt taagtcagga
    15241 acgcgccgcc cgtgcggaac tgctgcaata ccaattaaaa gaacttaacg aatttaatcc
    15301 gcagcccgga gagtttgagc aaatcgacga agagtacaaa cgtctggcga acagcggtca
    15361 attgctgacc accagccaga atgcattggc attaatggcc gacggtgaag acgcaaacct
    15421 gcaaagtcag ctttacacgg ctaaacaact ggtgagcgaa ttgattggca tggacagcaa
    15481 actgtccggc gtacttgata tgctggaaga agctaccatc cagattgctg aagccagcga
    15541 cgaactgcgc cactactgcg atcgtctgga tctcgatccc aaccgactgt ttgaacttga
    15601 acagcgcatc tcaaaacaga tttcgctggc acgtaaacat cacgtcagcc ctgaggcatt
    15661 accacagtat taccagtcgc tactggaaga acagcagcaa ctggacgatc aggccgactc
    15721 acaagaaacg ctcgcgctgg cggtaacgaa acatcatcag caggcactgg aaaccgcgcg
    15781 cgcattacac caacaacgcc agcaatatgc agaagaactt gcacagctga tcaccgacag
    15841 tatgcatgcg ctctcaatgc cgcatgggca gtttacgatt gatgtcaaat atgacgagca
    15901 tcacctgggc gctgacggtg ccgatcgtat tgagtttcgg gtaaccacca acccaggtca
    15961 gccaatgcag cctattgcca aagtcgcatc cggtggtgaa ttgtcccgca tcgcactggc
    16021 aatccaggtc atcacggcgc gtaaaatgga aaccccggca ctgatttttg atgaagtgga
    16081 tgtagggatt agcggtccaa cagcggcagt tgtcggcaaa ctgctgcgtc aactcggcga
    16141 atcaactcag gtgatgtgtg ttacccacct gccacaagtc gcgggatgtg gtcatcaaca
    16201 ctattttgtc agcaaagaaa ccgatggtgc gatgacagaa acacatatgc aatctctgga
    16261 taaaaaagcg cggttacaag agctggcgcg cctgcttggt ggcagtgaag tcacacgtaa
    16321 cacactggcg aatgcgaaag aactgcttgc agcgtaaact tttttcctgc ttcacggtca
    16381 gagtaaacag caaaacgccg taagaccgga aagcaaaagg ttttaaagtg atgaaaggtc
    16441 tattatcatc ggcatattac agatgagcca cgtactgctc gggcccgaaa aggaatcaaa
    16501 tcactatgcg ctgtaaaacg ctgactgctg cagcagcagt actattgatg ttaaccgcag
    16561 gctgttccac tctggagcga gtggtttacc gtcctgacat caaccagggg aactatctga
    16621 ccgctaacga cgtatccaaa atacgtgttg gcatgacgca acaacaagtt gcgtacgcat
    16681 tgggtacacc gctgatgtcc gatccatttg gtacgaatac ctggttctat gtcttccgcc
    16741 agcaaccagg tcatgaaggt gtaacgcagc aaacgctgac gctgaccttt aacagtagcg
    16801 gtgtgttgac caatattgat aacaaacctg cgctgagtgg taactaataa taaagttgct
    16861 ctcaaagacg ttaaaaaagg tgctcaatga gcaccttttt tctgtctgtt atttattcgc
    16921 tgatttttct gctcgttgcc tgcgaagctc tttcggatcg gcaatgagag ggcgataaat
    16981 ctccacccga tcgccatcat gcacactatc gcttagtttt gccggacggc tgtaaatgcc
    17041 gactttgttt ttagttaaat cgatatcggt acgcaattcc agcaagccac tggcgcgaat
    17101 agcttcttca accgtcgcgc cctcctgcag cgtcactcgc tgcaggtatt gcttctcagg
    17161 tagcgcataa gccacctcaa cggcaatttt acctagcact gtaaacctct ttcgcacgaa
    17221 ccgtaaaagc ctggaccata ttagccgcca gctctttaaa cacgcgacca aaggcgagtt
    17281 caatcaactt attggtaaac tcaaagtcga gatgaaactc gatacgacac gcctcctggc
    17341 tcagcggcgt aaacttccat ccaccaatca atttcttgaa cggcccatcc accagattca
    17401 taagaatact ttggttactg gtcaactggt tgcgggtagt aaacgttttg ctgatcccag
    17461 ccttagagac atctaccgcc gcagtcatct gcccaggagt ggactccaga atccgacttc
    17521 cggtacaacc tggcaaaaac tgaggataag actgaacgtc attcactaac tgatacattt
    17581 gctccgcgct gtagggtacc agtgcggtcc ggctaatctg aggcataaca atttccatca
    17641 acaaaaatcc aacaaataat atcatttatc ctgctgtaaa aaaaacgcta tcccggcgct
    17701 gggtaacatc gggttcatgc taagatagag ccttgtcccc cgcaggattg atatggggtg
    17761 ttttcgattt cagattaccg atgattcacg acgcttatga cgaagaaaaa agcacataaa
    17821 cctggttcag cgaccatcgc gcttaacaag cgcgcccgtc acgaatactt tatcgaagaa
    17881 gagttcgaag cgggacttgc cctgcaaggc tgggaagtta aatccctgcg cgcaggaaaa
    17941 gccaatatca gcgacagcta cgtccttctg cgtgacggag aggcatttct gtttggcgct
    18001 aacatcacgc caatggccgt ggcctccacg catgtggtgt gcgatcctac ccgtacccgc
    18061 aagttacttc tcaaccagcg cgaactggac tcattgtacg gtcgcgtcaa tcgagaaggc
    18121 tataccgtag tggcgctctc cctgtactgg aaaaatgcct ggtgcaaagt gaaaatcggc
    18181 gtcgccaaag gtaaaaaaca gcacgataaa cgttcagata tcaaagaacg cgaatggcag
    18241 gtggataaag cacgcatcat gaaaaacgcc caccgttaaa cctgcactcc aattattgac
    18301 cagttcctca ccgcgcctcc ctctccggcg gcgcgaatga acatcttatt ggctatcaca
    18361 tccgacacaa atgttgccat cccattgctt aatcgaataa aaatcaggct acatgggtgc
    18421 taaatcttta acgataacgc cattgaggct ggtcatggcg ctcataaatc tggtatactt
    18481 acctttacac attggggctg attctggatt cgacgggatt tgcgaaaccc aaggtgcatg
    18541 ccgaggggcg gttggcctcg taaaaagccg caaaaaatag tcgcaaacga cgaaaactac
    18601 gctttagcag cttaataacc tgcttagagc cctctctccc tagcctccgc tcttaggacg
    18661 gggatcaaga gaggtcaaac ccaaaagaga tcgcgtggaa gccctgcctg gggttgaagc
    18721 gttaaaactt aatcaggcta gtttgttagt ggcgtgtccg tccgcagctg gcaagcgaat
    18781 gtaaagactg actaagcatg tagtaccgag gatgtaggaa tttcggacgc gggttcaact
    18841 cccgccagct ccaccaaaat tctccatcgg tgattaccag agtcatccga tgaagtccta
    18901 agagcccgca cggcgcaagc cctgcgggct tttttgtgct ccgaatttgt cccgcgaagt
    18961 ctgatgccaa ctaattaaat ccgatccttt taggcacctt gttaggcact tcataaagct
    19021 atattgtctt tgaggtgcct aaaactatgg aaacccggca atggcaagac aaaccaaacc
    19081 tctatccgtt aaggaaatcg aatctgctaa acccaaggaa gcggactacg ttctctatga
    19141 tggcgatggc cttgagctac tcattaaatc cagcgggagt aaaatctggc agtttcgcta
    19201 cattcgccct gtcaccaaga cacgagcaaa gaaaagcata ggcccttacc cgtcagttac
    19261 ccttgccgat gccagaaact accgtgcaga gtcccgctca ctcctagcta aacagattga
    19321 gccgcaggaa catcagcaag aacaattgcg tagttcgctg gaagccaaaa ccaatacttt
    19381 ccagctcgta gctgaacgtt ggtggaatgt gaagaaagcc agtgtgacag aggactatgc
    19441 ggaagatatc tggcgctctc ttgaaagaga tgtctttcct gcgattggcg acgttagcgt
    19501 tacagatatt aaagctcata cactggttca ggccgtacaa ccggttcagg ccagaggagc
    19561 actggaaacg gttcgtcgcc tgtgtcaacg tattaatgag gtcatgatct atgcccagaa
    19621 cacagggctg attgatgctg ttcccagcgt taatatcggt aaagccttcg aaaagcctca
    19681 gaaaaagaac atgcccagca ttcgaccgga tcagctatct caactgatgc agacaatgcg
    19741 aacagccagc attagccttt ccacacgctg cctgttcatg tggcaacttc ttactattac
    19801 ccgccctgcc gaagcggctg aagctcgctg ggaagaagta gacatagaag cgcaagagtg
    19861 gaagattccc gcagcacgca tgaaaatgaa ccgcgaccat actgttccat tgtcagatga
    19921 agcaatagct gtgctggaga tgatgaaacc gctaagcggc aaccgagaat ttatctttcc
    19981 tagccgtatc aagcccaacc agcctatgaa cagtcagact gttaacgcct cactaaaacg
    20041 cgcaggtttt ggtggtgtgc ttgtttcgca tggtctgaga tcaattgcca gtacggctct
    20101 taatgaacaa ggctttccgc ctgatgccat tgaggcagca ttggcccatg tggataagaa
    20161 tgaggttcgt cgtgcttata accgcagcga ttacctggaa cagcgtcgcc cgatgatgca
    20221 atggtgggca aattttgtga tggcagcgga tcgtggaagc atgattgaag gtgggataaa
    20281 aggaatgaag ctagttgggt gactaaatag ctgcgcggca tagtgtccca ctttcagcca
    20341 gcccttgcag gttaagccac cagacagtaa acggcgggtg tcgcgttatt aggcgcagct
    20401 atttagtagt aatattagga ttggtgattc tgcac
//
LOCUS       DAJTQY010000074        20287 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.074, whole genome shotgun sequence.
ACCESSION   DAJTQY010000074 DAJTQY010000000
VERSION     DAJTQY010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20287
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.074"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(60..818)
                     /gene="aroD"
                     /locus_tag="OO349_002755"
     CDS             complement(60..818)
                     /gene="aroD"
                     /locus_tag="OO349_002755"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HCR5867760.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIAHVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLVGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(849..1715)
                     /gene="ydiB"
                     /locus_tag="OO349_002756"
     CDS             complement(849..1715)
                     /gene="ydiB"
                     /locus_tag="OO349_002756"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HCR5867761.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLVCEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTDTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(1727..2998)
                     /locus_tag="OO349_002757"
     CDS             complement(1727..2998)
                     /locus_tag="OO349_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5867762.1"
                     /translation="MRTVTKLSFSTPFILAVLCIYFSYFLHGISVITLAQNMTSLAEK
                     FSTDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPN
                     LTLAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSY
                     MLLNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGV
                     SSVLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLK
                     KMVRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEF
                     FPKSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFS
                     RYYRVFIIPENDVQFGERKFSTRLNTIKHRG"
     gene            complement(3181..4386)
                     /locus_tag="OO349_002758"
     CDS             complement(3181..4386)
                     /locus_tag="OO349_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5867763.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFDILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSTGSIATFTIPLITAHLSQRSIADIMWFDTAIGFLLALFIGLRSRKKTRHHS
                     LKENVAPGG"
     gene            complement(4486..4842)
                     /locus_tag="OO349_002759"
     CDS             complement(4486..4842)
                     /locus_tag="OO349_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HCR5867764.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(4978..5085)
                     /gene="rprA"
                     /locus_tag="OO349_002760"
     ncRNA           complement(4978..5085)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="OO349_002760"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(5271..6383)
                     /gene="ydiK"
                     /locus_tag="OO349_002761"
     CDS             complement(5271..6383)
                     /gene="ydiK"
                     /locus_tag="OO349_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HCR5867765.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            6772..9828
                     /locus_tag="OO349_002762"
     CDS             6772..9828
                     /locus_tag="OO349_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCR5867766.1"
                     /translation="MIPQISLAPGVVQLVLNFLQELEQQGFTGDAATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVEGIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESHPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            9825..10235
                     /gene="menI"
                     /locus_tag="OO349_002763"
     CDS             9825..10235
                     /gene="menI"
                     /locus_tag="OO349_002763"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HCR5867767.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            10335..10523
                     /locus_tag="OO349_002764"
     CDS             10335..10523
                     /locus_tag="OO349_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HCR5867768.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            11071..11439
                     /gene="sufA"
                     /locus_tag="OO349_002765"
     CDS             11071..11439
                     /gene="sufA"
                     /locus_tag="OO349_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HCR5867769.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQSMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFRV"
     gene            11448..12935
                     /gene="sufB"
                     /locus_tag="OO349_002766"
     CDS             11448..12935
                     /gene="sufB"
                     /locus_tag="OO349_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HCR5867770.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            12945..13691
                     /gene="sufC"
                     /locus_tag="OO349_002767"
     CDS             12945..13691
                     /gene="sufC"
                     /locus_tag="OO349_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HCR5867771.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            13666..14937
                     /gene="sufD"
                     /locus_tag="OO349_002768"
     CDS             13666..14937
                     /gene="sufD"
                     /locus_tag="OO349_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HCR5867772.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGDISPQQRDSLALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            14934..16154
                     /gene="sufS"
                     /locus_tag="OO349_002769"
     CDS             14934..16154
                     /gene="sufS"
                     /locus_tag="OO349_002769"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HCR5867773.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNSIAEYEQNLMHYALSQLESVPDLTLYGQQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVE
                     RLVTGLQRIHRLLG"
     gene            16167..16583
                     /gene="sufE"
                     /locus_tag="OO349_002770"
     CDS             16167..16583
                     /gene="sufE"
                     /locus_tag="OO349_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HCR5867774.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRAEDGSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            16732..17736
                     /gene="ldtE"
                     /locus_tag="OO349_002771"
     CDS             16732..17736
                     /gene="ldtE"
                     /locus_tag="OO349_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HCR5867775.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(17799..18035)
                     /gene="lpp"
                     /locus_tag="OO349_002772"
     CDS             complement(17799..18035)
                     /gene="lpp"
                     /locus_tag="OO349_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HCR5867776.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(18346..19758)
                     /gene="pykF"
                     /locus_tag="OO349_002773"
     CDS             complement(18346..19758)
                     /gene="pykF"
                     /locus_tag="OO349_002773"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HCR5867777.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEKGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
BASE COUNT         5314 a         4855 c         5176 g         4942 t
ORIGIN      
        1 ggttaaaaat acttttttta tttcggcggg ggagggtatt tccgccgaaa tattattgct
       61 tatgcctgat gtaaaatagt taataccgtg cgcaaatcat ttaccgagat ttgccctggc
      121 gcagaggctt tttttaccgc accaaaagtt gccgccgagc caaatacttc accaaccaga
      181 cgagaaatta cgccagtttt tgccatcgac atcgtgatga ttggacgatc ggcatactgc
      241 tcctgcatct ccagggtcgc ggcaagcaac gtcagcacat cgctggtact ttgcggcatc
      301 agcgcaatct taggaatatc ggcgtcgaag gactgcattt tgcgcagacg ggcaatgatt
      361 tcttcggctt ccggcgtttt atggaagtca tggttggaca tgacaacttt cacatcatgc
      421 gcgtgggcgt aggcgacggt ttctttgacc tgatcatcgc cggtaaataa ctccagatcg
      481 atcatatcaa ccaggccgct gtcgatggct gcacgattga gagcaatata agcctcggtg
      541 gaaatcgcct gctcgccgcc ttctttggca ctgcggaagg taaacagcag cggtttttct
      601 ggcatggttt cacggagaat ttttgccgcc gccatgacag actccacatt ggagaggtcg
      661 gcaaagtggt ccacacgcca ttccagaata tcaaagtccg cttcacgata ggcgagagct
      721 tcggatttca cgtgggcgat atctttcgcc atcagcgaga cgatgatttt aggtgcgccc
      781 gcaccaatga cgagatcttt tacagttacg gttttcattt tttacccttt ctgcacgcgg
      841 tcagcctgtc aggcaccgaa ccccatgacc tgtttaacat attccagagg gaaatctttg
      901 ccagtccata acgtgaactg ttcagcccct tgccacaaca acatgccgta tccatcaatc
      961 gttttgcaac cagcttgttg tgcctgctgc aataacttcg tcatatgcgg gttatacacg
     1021 cattcagtga ccagcagtcc cggatgtaac agactgatat cattaaccaa tgattcattc
     1081 tcaaggggtt tcatacctac ttttgtgcca ttggttaaaa tgtcggcgga agccagggct
     1141 tcagcaaagg cttgctgatc ggcgagatcg gtatccgtga cgacacaatc ggtgttttcg
     1201 ttaacccgct gcgcgaaggc gagggcttta tcgaagaact catcccgacg gttaaagagt
     1261 ttaatttctt ttaaaccttc aattgccccc tgcgcgccaa tcgccgttga ggcacctccg
     1321 gcccctaaca gcaccatcgt tttgcctttg atatcaaaac cgctctcttt aatggcgcga
     1381 atatggcccg tgccgtcggt gttatagcca cgcagatagc catcatcatt aacgatggtg
     1441 ttgatggcac ccaccagttt ggctgctggc gttaattcat caacatattc acacaccagt
     1501 tgtttgttcg gcatcgacac accagttccg cgcattttga gggcttttaa tccttcaatt
     1561 gctccgggaa agctatcgtt atccacttcg aaggccatat aggtaaatgg caatcccgct
     1621 ttttctaagg ctttattctg catttcaggc gataaactgt ggcggatagg ataagccatc
     1681 aatccaatca attcgtattt tgcggtaaca tccataatta actcctttaa cctctatgct
     1741 taattgtgtt taaccgggtg ctaaatttgc gttcgccaaa ctgcacatca ttctcaggaa
     1801 taataaaaac gcggtaatag cggctaaaaa caataattgc ggtaatcagg gccagcagcg
     1861 cgaaagtaaa atcgagaaca atgatatatt gcaggccgat gttcgacaga taaccggtaa
     1921 tcagcggaat aacaaagtta gccagtccac ccatcatcat ataaatactg gtgactttgg
     1981 ctttgctttt ggggaaaaac tctgacatga ccgaaacacc gagctgtaaa atgccgccag
     2041 ctgctgagaa accgataaca aaggcgccgg cattacacac cagtggagaa gggtacaggt
     2101 agataatggc agctgttatt gttgccagcg cagagttaaa tacattagcc cagatgggcc
     2161 ggaccatttt tttcagtagt gcggcaaaaa taaagacaca gaccaacgag cccatactgt
     2221 aataagagat ggtttttaat gcctcagctt ctgacatacc agcaaaagcc atcgcatatt
     2281 tgggcatcca caccacaatc acataaaagg tcgagaatgc ggctacaccg aacagtaccg
     2341 atgaaacacc ttccagccag actaaaggtt tgctgttcat ttgcggtaat tcattagcta
     2401 cgctggcgtc caccaactgg ctggggaatt tgcttttcaa cagcatcagc gtgatcagta
     2461 caaacagaat acccggaata atcagcccat agccgtacca gatattattg agcaacatat
     2521 agctcaccag cattgggtag aacatttgcc caaatgacac catcgcttta accagtatga
     2581 ccgccgaacc agaggctttc ggaaagcatt ccatgagcgc ggggtagcca cccgtatcca
     2641 gcgctgagtt agcgatacct acgcacactg ccagaccgta ggcgagagtt aaattcgggc
     2701 aagcgggaat accaaagaag aatagcagat acattattac tgccattaat atcaccgccc
     2761 gacgaccaaa cttatcggag atcacaccga agaataaaat actgatcaat cgccccaaac
     2821 cgataccgga aattaaatag gcaatgcccg cgttgtcagt ggaaaacttt tctgccagag
     2881 atgtcatatt ttgggcaagc gtaataacac taatgccgtg caggaaatag ctgaagtaaa
     2941 tacaaagaac agccaggata aatggcgtgc tgaaagataa tttcgttacc gtacgtattg
     3001 ttctccgacg gtttacataa ttactatact gaagatgatt ttccctgggt acatggcata
     3061 aagtttatgt ttccaggaat attgcatgtc atagtttgtt tttgatcgcg attttcctta
     3121 gttagcgcag aaagagttat ttatccgtaa gatcttgcat gacctgaaaa gaatattgca
     3181 ttacccaccc ggagcgacat tttcctttag cgagtgatgc cgcgtttttt tgcggctgcg
     3241 taagccgata aacagtgcca gtaaaaaacc gatggcagta tcgaaccaca taatatcggc
     3301 aatacttctt tgggacagat gagccgtaat caacggaata gtaaaggtcg caatactgcc
     3361 cgtactgtaa tagatccctg tagctttacc tttagcgtaa gggaaacgtt cagccattaa
     3421 cgtcaggcca atttgcacaa cacctccagc agaggtaaaa ccaatgacaa aagcaaatat
     3481 tatcaccaca taaaatgtgg gatgcaggca gacggtaaac agagcaataa atgagataaa
     3541 ggtgtacagc atcagtaatg ttgttgggcg aacggtatta cgaatgagtg gagcggtaat
     3601 aaatacacaa agcagcgaac ccacggtgta gatactgagt agtttgatcg acatagtgta
     3661 tgacatgcct gcaacaaatt gtccgtactg tgccagccac tggctaacca gataaaacgt
     3721 tgccattgag atatagccat ataaggtata actggctaaa tcgataattg aacagcgatg
     3781 ttccgtagag ctggtggttt tctttatgac aggtaagcga cgacccggat ggggtgggaa
     3841 cgtacaacgg tataaaaaca gagcgttaat aaacataatg cctgcagcaa tcataaagga
     3901 ccaaccgaac cacagttcag cccacactaa caggctaatg attagcggta ataaaaattg
     3961 tccgctggaa acaaatgctt taattaaaat attggctgtc ccaggtgagc gtggaaaagc
     4021 ttccatcaaa ctgggataag tgcctgcatc gagaaaactg tttgccattc ccgccagaaa
     4081 gccaaaaaca taagcgataa tgatgttatt ggtctgcagg atgtcaaaaa agaaggccat
     4141 atagcagcac atcccgagca tgataaaagg gcggcgacca aagcgatcgg ataataatcc
     4201 tgcaaaaagc aagacactta atcgaccaat gcccagcgat gagataacta tcgagacacc
     4261 cgcggcatta gtctgccaaa gtgtctccag cgaggccata ttcaggctca tcaaaatgac
     4321 gcccatacca tgcaccaggt aattaaaata caacccaagt gcggtagggt aatagggatt
     4381 tttcataggt tctacttagc aaaggtgctt taaaaagtgt agcgatgttg tgtcagtatc
     4441 cattcattat tgttgtaaca acgacaattc ctttctgtgg aaaaattaac aaagccgctc
     4501 cagatcgtgt gcatataatt cagcggctac cgattgatag atcttccagt caataaaatt
     4561 gccatcaggg tagacttgtt gaaacgcggt taagtcgggg aaaaaacgca tggtgttgtt
     4621 gccgatgcgg ttatttattt tctctatgat ggcatgaaga tgtttttttc gttgctgacg
     4681 catagccaac agttgctcga ctacacaatc aggaatggcg catttgccat tttcccactg
     4741 acgccagctt tcactgtcac cggtctgggc aatccaggtc gcgcattcat caatagtcat
     4801 ggcaaaaata tggcggagtg cctggagttc ataagcgttc atcgtgtaat ggctgcatga
     4861 aataaacaat aaaatataaa taacattatc agcctgctga cggcttgaag agagtcacag
     4921 tatcttgtgc aacattattg gaaagagtga ggggcgaggt agcgaagcgg aaaaatgtta
     4981 aaaaaaagcc catcgtggga gatgggaaaa gactacacac agcaattcgt tgtttcactc
     5041 aggggatttc catgcttata aatcaatatg ttgatttata accgtgagct aatagtaggc
     5101 atacggactt tttgcgtcga tcagattcgt ctcattagtt taaaaactgt aaagaatttg
     5161 cgacaggtac taccagctga atggcggtaa gcaggaaaat atggggaaga gcaagcaaat
     5221 atcaattagc ggtgattaac aatcaggcgg cagagccgcc tgatgagaaa ttacttattc
     5281 ggtttctcga tttcgccaag ttcttcgaga atttcttccg gttggtccgt cggtggcggg
     5341 acttcttcca cccacgcggc aaacagacgc caggaaacgg ctaacagaac cggaccaata
     5401 aacagaccga tcatcccgaa agcaatcaaa ccaccaataa cgccagagag gatcaggatc
     5461 agcggtaaat cggcacccat gcgaattaac attgggcgga tgacgttatc cagcgtgcca
     5521 accacaccgc tccacactaa caataccgtt ccccaggtgg tatcgccggt ccagtagagc
     5581 cagataatcg ccggaatcag taccggcaac gggccaagct ggacaaggca ggagaggatc
     5641 attaacaccg ttagcaacgt tgcataaggt acgccggata cggcgaggcc gataccgcca
     5701 agcactgcct gtactaacgc cgtcaccacc acacccagcg ccaccgcgcg gatagcctgt
     5761 gccgccagca gcacggcggc atctccgcga acgcctgcca gacgggttgc aaaatggcga
     5821 atgccttgtg ccacctgttc accgcgccag tacagcaggg cactgaagag aagcatcagc
     5881 gcacaatgca ccataaagcg cccgatatgc gccgcctgcc caacgaacca ggtggtggtg
     5941 gtgccaatat aagggcggac tttcgccatg atcgccgtac cccccatatc cagcaagttg
     6001 tgccagcctg catacagctt cgcgccaatc accggaatgg tattaagcca cgccagatcg
     6061 ggtaacgtca tgtcgccgct ggaaatggct ttaattagcg ggccgctgcc gtcgacgata
     6121 ctgttaacca gcaaagcaat agggatgata aacaccatca ctaataacag cgtcatcacc
     6181 agaacggcga gagagcggcg gccaaacatg atcttttgca aacgtaacaa taccggccag
     6241 gtggcgataa ccaccgtacc ggcccatgca aagccgagaa taaagggttg aacaatccac
     6301 agacatgcca caatcatgat ggctaaaaac agcaccgaaa gcagaatttg tgcgacatcc
     6361 ctgggctgac gaacatttac cataactact tttcaccttt tttgtgcgtc aaatcgtcga
     6421 cgcgacgtga acacgcgaaa ctcacctgca taatgatgag caatttcagc ggtttttaac
     6481 aggccgattc tgcccttaat tctgatgggc gtgttagaaa aaaatgtggt acaacaactc
     6541 aggcaacacg caaacgattt actcgtcgta tttcaagttg catgtgctgc ggctacgttc
     6601 gttcacccca gtcacttact attgtaagct cctggagttc actcgcttgc cgccttcctg
     6661 caactcgaaa tccatagagt aaataaacat cacctttcag tgcgctattg cagccgcctg
     6721 aaagaatagt gtatcgacct acatcacaga ccgcaggaaa gggtcaatat aatgattcca
     6781 cagatttccc tggcacccgg tgtcgttcaa ctggtgctta attttttgca agagctggag
     6841 caacaaggtt ttaccggcga tgcggcgaca agttatgccg atcgtctgac aatgtcgacc
     6901 gacaacagta tttaccaact tctccccgat gcggtggtat ttccgcgttc aaccgcagat
     6961 gtggcgctga tcgcccgtct tgccgcgcag gaacgctatt catcgctgat ctttaccccc
     7021 cgcggcggcg gcaccggcac taacggtcag gcgctcaacc aggggattat tgttgatatg
     7081 tcccgccata tgaaccgcat catcgaaatt aaccctgaag agggctgggt gcgcgttgag
     7141 gccggggtga taaaagacca actcaatcag tacctgaaac cgttcggcta cttttttgcg
     7201 ccggaacttt cgaccagcaa ccgggcaacg ctcggtggga tgatcaatac cgatgcatcc
     7261 ggtcagggat cgctggtcta tggcaaaacg tcagatcacg tacttggcgt acgcgcggtg
     7321 ttgttggggg gcgatattct cgatacgcaa cctttacccg tcgagctggc ggaaacgctg
     7381 ggtaaatcca ataccacaat cgggcgaatt tataacacgg tttatcaacg ttgccgtcag
     7441 caacgccagt taatcatcga caacttcccc aaacttaacc gctttcttac cggttacgat
     7501 ctgcgtcatg tctttaacga tgagatgacc gagttcgacc tgacgcgcat tctgacgggt
     7561 tcagaaggga cgctggcctt tattaccgaa gcgcggctgg atattacgcg cttgcctaaa
     7621 gtgcgccgtc tggtgaatgt caaatatgac tcttttgact ctgcgctgcg taacgcgccg
     7681 tttatggttg aggcgcgggc gctttcggta gagacggtgg actcaaaagt gctgaatctg
     7741 gcgcgggaag atattgtctg gcattccgtc agcgagttga ttaccgatgt gcctgacaaa
     7801 gagatgctcg gactgaacat tgtggaattt gctggtgatg atgaggcgct gattgacgag
     7861 cgagttaatg cactctgtgt gcggcttgat gagctgattg ccagccaaca ggcaggtgtg
     7921 attggctggc aggtgtgccg cgagctggcg ggggttgaag gtatctatgc gatgcgcaaa
     7981 aaagccgttg gtctgcttgg caatgccaaa ggtgccgcta agccaattcc gtttgctgag
     8041 gatacctgcg taccgccgga acatctggcc gattatattg ctgaatttcg cgcgctgctc
     8101 gacagccacg gcttaagcta cggtatgttc ggtcacgtcg acgcaggtgt cttgcacgtc
     8161 cgtccggcac tggatatgtg cgatccgcaa caagagattt tgatgaagca aatctctgat
     8221 gacgtggtgg cgctgactgc gaaatacggt ggtttgttgt ggggcgagca cggcaaaggt
     8281 tttcgcgctg aatacagccc ggcgtttttc ggtgaggaac tttttgcaga actgcgcaaa
     8341 gtgaaagcgg catttgaccc gcataaccga ctcaacccag ggaagatttg cccgccagaa
     8401 ggtctcgatg cgccgatgat gaaagtggac gcggtgaagc gcggtacatt cgatcggcag
     8461 atccccattg cggtacgcca gcagtggcgc ggtgcgatgg agtgtaacgg caacggttta
     8521 tgcttcaact ttgatgcccg tagtccgatg tgtccgtcga tgaagatcac ccagaaccgg
     8581 attcattcac cgaaagggcg cgcaacgctg gtgcgtgaat ggctgcgctt gttggcggat
     8641 cgcggcgttg acccactcaa actggaacaa gaactgcctg aatccggcgt cagtttgcgg
     8701 acgttaattg cccgcacgcg caatagctgg catgcgaata aaggcgaata tgacttctca
     8761 cacgaagtca aagaggcgat gtcgggctgt ctggcctgta aagcttgttc gacccagtgc
     8821 cccatcaaaa ttgatgtgcc ggagtttcgc tctcgttttc tgcagctcta tcacacccgt
     8881 tatttacgcc cgcttcgcga ccacctcgtt gctacggtcg agagctacgc accgctgatg
     8941 gcacgcgcgc cgaaaacctt taacttcttc attaaccagc cgctggtgcg caaactctcg
     9001 gaaaaacata tcggcatggt tgatttgcca ctgctgtcgg tcccctcgct acaacaacaa
     9061 atggtggggc atcgctcggc aaacatgacg ctggaacagc ttgaagctct caatgcagag
     9121 cagaaagcgc gcacggtgtt ggtggtgcag gaccccttta ccagctatta cgatgcgcaa
     9181 gtggtggcgg attttgtccg tctggttgaa aaattaggtt tccagcctgt gttactgcca
     9241 ttttcgccaa atggcaaagc ccagcatatc aaaggctttc ttaatcgttt tgcgaagacg
     9301 gcgaaaaaga cggcggattt cctcaaccgt atggctaagc ttgggatgcc aatggtgggc
     9361 gtcgatccgg cgctggtact ttgttatcgc gatgaatata aactggcgct gggcgaggaa
     9421 cgtggcgagt ttaacgtctt acttgcgaat gaatggctgg caagcgcact tgagtcacat
     9481 ccagtggcta cagtcagcgg tgaatcatgg tatttctttg gtcactgtac cgaggttacc
     9541 gctttgccgg gcgcgccagc acaatgggcc gcgatatttg cccgttttgg cgcgaaactg
     9601 gaaaatgtca gcgtgggttg ctgcggcatg gcagggactt acggacatga agcgaaaaac
     9661 catgaaaatt cgctcgggat ctatgagtta tcctggcatc aggctatgca gcgactgccg
     9721 cgtaaccgct gtctggcgac cggatattcc tgccgtagcc aggtaaaacg ggttgaaggc
     9781 acgggggtac gccatcctgt gcaggcttta ctggagatta ttaaatgata tggaaacgga
     9841 aaatcaccct ggaagcactg aatgctatgg gtgaaggaaa catggtgggg ttcctggata
     9901 ttcgctttga acatattggt gatgacaccc ttgaagcgac aatgccagta gactcgcgga
     9961 caaagcagcc tttcgggttg ctgcatggag gagcatccgt ggtactggcc gaaagtatcg
    10021 gttccgttgc cggttattta tgtaccgaag gtgagcaaaa agtggttggt ctggaaatca
    10081 atgctaatca cgtccgctcg gcacgagaag ggcgggtgcg cggcatatgt aaaccgttgc
    10141 atctcggttc gcgtcaccag gtctggcaga ttgaaatctt cgatgagaaa gggcgtttgt
    10201 gctgttcgtc acgattgacg actgccattt tgtgacgttg tcaccagaaa agtgtgacgc
    10261 atattgcttt tggtcattat ctgcacagta aacggtggta tactgtgcag gtcttgtcta
    10321 aaagcgagga tgctatgtct actcaacttg atcccaccca gctggccatt gaatttttac
    10381 gccgtgacca gagcaatctt tcacctgccc aatatctcaa acgtctgaaa caactagaac
    10441 ttgaatttgc tgacctgctt actctctctt ctgctgaatt aaaagaagaa atctactttg
    10501 cctggcgtct gggcgtgcat tgatcctgac ttaatgccgc aagttcgcca aatcgacctg
    10561 gattaacctc cgtccaggtt cgatgacctt cttaatactg cttatctacc gcctgatatt
    10621 gttcaaaaat aatactaatc gcagtttgtg ttaaaacggc gggttagctt tatgagatta
    10681 ttcttatcgc cccttcaaga gctaagccac tgagagtgcc ggagataagc gccggatggg
    10741 gtagaaaccc ttaagcctgt gtcgcacaga cttaagggtt ttcttatttc tgaacgctgc
    10801 acttttttac ctatctctta aatagtcatt ttcaatacaa ctttttcttt ttcatcacag
    10861 ccttaatgct ccttagataa taaccattat ctaacaatga gatacctaat tcttagcgtg
    10921 cctgttaacc cacacatcag gggctatgct tattaaaagc aggcaaaaag aatgttttac
    10981 ggtaaagccc ctgcgtttgc tgggttgaac tgataatcat tatcactaac atgctgttat
    11041 acgctgaaag cgatgaagtg aggtaaatag atggacatgc attcaggaac ctttaaccca
    11101 caggatttcg cctggcaagg cttaacgctg acacccgcag cggcgataca catccgcgag
    11161 ctggtggcaa agcagcctgg tatggtcggc gtgcgcttag gtgtgaagca aacgggctgc
    11221 gcgggctttg gctatgtgct cgacagtgtt agcgagccgg acaaagacga tctgctgttt
    11281 gaacacgacg gcgcgaagct gtttgtcccg ctgcaatcga tgccgtttat tgatggcacg
    11341 gaagtcgatt tcgttcgtga aggacttaat cagatattca aatttcacaa ccctaaagcc
    11401 cagaatgaat gtggctgtgg cgaaagcttt agggtatagg cggtactatg tctcgtaata
    11461 ctgaagcaac tgacgatgtc aaaacctgga ccggcggccc gctgaattat aaagaaggat
    11521 tcttcaccca gttagccacc gatgagctgg caaaggggat aaacgaagag gtggtgcgcg
    11581 caatttcggc gaagcgtaat gagccggagt ggatgctgga gtttcgtcta aacgcctatc
    11641 gcgcatggct ggagatggaa gaaccgcact ggttgaaagc gcactacgac aagctgaatt
    11701 atcaggatta cagctactac tcagcaccat cgtgcggtaa ttgtgacgac acttgcgcgt
    11761 ctgaacctgg cgcggtgcag caaactggcg cgaacgcctt tttaagtaaa gaggtggagg
    11821 cggcgtttga gcagttgggc gttcccgtgc gggaaggcaa agaggtggcg gtggatgcca
    11881 ttttcgactc tgtttcggtt gccaccactt atcgcgaaaa actggcggag cagggaatta
    11941 ttttctgttc ctttggtgag gcgatccacg atcacccgga actggtgcgt aaatatctcg
    12001 gcaccgtggt gccggggaat gacaacttct ttgcagcact taatgcggcg gtagcctctg
    12061 acggtacgtt tatttatgtg cctaaaggcg tgcgctgccc gatggaactt tccacctatt
    12121 ttcgcattaa cgcggaaaaa accgggcagt ttgagcgcac aattctggtg gccgacgaag
    12181 acagctacgt cagctacatt gaaggctgtt ccgctccggt gcgtgacagc tatcagttgc
    12241 acgcggcagt ggtggaagtc atcatccata aaaacgccga ggtgaaatat tccacggtac
    12301 aaaactggtt ccctggcgat aacaacaccg gcggtattct caacttcgtc accaagcgtg
    12361 ctttgtgcga aggcgaaaac agcaaaatgt catggacgca atcagaaacc gggtcagcga
    12421 ttacgtggaa atatcccagc tgcattttgc gcggcgataa ctccattggt gagttttact
    12481 cagtggcgct gaccagcggt catcagcaag cggataccgg caccaagatg atccacatcg
    12541 gtaaaaacac caaatcgacc attatctcga aagggatctc tgccggacat agtcagaaca
    12601 gttatcgcgg cttagtgaaa atcatgccga cggcaaccaa tgcgcgcaat ttcactcagt
    12661 gcgactcaat gctgattggc gctaattgtg gggcgcatac cttcccgtat gtcgaatgtc
    12721 gcaataacag cgcacaactg gagcacgagg caacgacatc acgtattggt gaagatcaac
    12781 tgttttactg cctgcaacgc gggatcagcg aagaagacgc catctcgatg attgttaacg
    12841 gtttctgcaa agacgtgttc tcagaactgc cgctggaatt tgccgttgaa gcacaaaaac
    12901 tcctcgccat cagtcttgaa cacagcgtcg gataaggaat aaacatgtta agtattaaag
    12961 atttacacgt cagcgtggaa gataaagcta tcctgcgtgg attaagcctc gacgttcgtc
    13021 ccggcgaagt tcacgccatt atggggccaa acggttcggg caaaagtacc ttatcggcaa
    13081 cgcttgccgg gcgcgaagat tatgaagtga cgggcggcac ggttgagttc aaaggcaaag
    13141 atttgcttgc actgtcgccg gaagatcgtg caggcgaagg catctttatg gccttccagt
    13201 atccggtgga gattccaggc gtcagtaacc agtttttcct gcaaacggca cttaatgcag
    13261 tgcgcagcta tcgcggccag gaaacgctcg accgctttga ttttcaggat ttgatggaag
    13321 agaaaatcgc tctcctgaag atgccggaag atttattaac ccgttcggta aacgttggtt
    13381 tttccggcgg cgagaaaaag cgcaacgata ttttgcaaat ggcggtgctg gaaccggagt
    13441 tatgcattct tgatgagtcg gactccgggc tggatattga cgcattaaaa gtggtcgccg
    13501 atggcgtgaa ctcgctgcgt gatggcaagc gctcattcat cattgttacg cactaccaac
    13561 gcattctcga ctacatcaag cctgattacg ttcatgtgct atatcaggga cgaattgtga
    13621 aatccggcga tttcacgttg gtcaaacaac tggaggagca gggttatggc tggcttaccg
    13681 aacagcagta atgcgctgca acagtggcat cacttgtttg aagctgaagg gacaaaacgc
    13741 tcaccgcaag cacagcagca tttacaacaa ttgctgcgta ccggactgcc gacacgtaaa
    13801 catgaaaact ggaaatatac gccgctggaa gggctgacca atagtcagtt tgtcagcatt
    13861 gcgggagata tatccccaca gcagcgtgat tccttagcgt taacgttaga cgccgtgcgg
    13921 ctggtgtttg tcgatggacg ttacgtgccc gcgctgagcg atgcaattga aggcagcgga
    13981 tatgaagtga gcattaacga cgaccgtcag gggttacccg acgctattca ggcggaagtg
    14041 tttctgcatt tgacggaaag cctggcacaa agcgtgacgc atatcgccgt gaagcgcggt
    14101 caacggccgg caaagccatt gctgttaatg catatcaccc agggcgtggc aggtgaagag
    14161 gtgaacactg cccattaccg gcatcatctg gatctggcgg aaggtgccga agcaacggtg
    14221 atcgaacatt ttgtcagcct taatgatgct cgtcatttta ccggggcacg gttcactatc
    14281 aatgtcgcag cgaacgccca cttgcagcat atcaagctgg cgtttgaaaa cccggtcagt
    14341 caccactttg cccataacga tttgttgctg gctgacgatg ccaccgcatt tagccatagt
    14401 ttcctgctgg gtggcgcagt gttacgacat aacaccagta cgcaactcaa tggcgaaaac
    14461 agcacgctgc ggatcaatag cctggcgatg ccggtgaaaa acgaggtgtg tgatacccgt
    14521 acctggctgg aacacaataa aggtttttgt aacagccgac agttgcacaa aactatcgtc
    14581 agcgacaaag gccgcgcggt atttaacggt ttgatcaacg tcgcgcagca cgccatcaaa
    14641 acggatggtc agatgaccaa caataatctg ctgatgggca aactggcgga agtggataca
    14701 aaaccgcagc tggaaatcta tgcagatgat gtgaaatgca gccacggcgc gacggtgggg
    14761 cgtattgatg atgaacagat gttctatctg cgctcgcgcg ggatcaatca gcaggatgcc
    14821 cagcagatga tcatttacgc ctttgctgcc gaactgacgg aagcactgcg tgatgagggg
    14881 cttaaacagc aggtgctggc ccgaatcggt caacggctgc caggaggtgc aagatgactt
    14941 tttccgtcga caaagtgcgg gccgactttc cggtgctttc gcgtgaggta aacggtttgc
    15001 cgctggctta tctcgacagc gccgccagtg cacagaaacc gagccaggtg attgacgccg
    15061 aggccgagtt ttatcgtcat ggctacgcgg cggtgcatcg cggtattcat accttaagcg
    15121 cccaggcgac cgagaaaatg gagaacgtgc gcaagcgggc atcgctgttt attaatgccc
    15181 gttcggcgga agagctggtg ttcgtccgcg gcacgacgga agggatcaat ctggtcgcca
    15241 atagctgggg caacagcaat gtgcgggcgg gcgataacat catcatcagt cagatggagc
    15301 accacgctaa cattgtcccc tggcagatgc tttgcgcacg cgttggcgca gagctgcgtg
    15361 tgatccctct caaccccgac ggtacgctgc aactggagac gctgcctacg ctgtttgatg
    15421 agaaaactcg cctgctggca attactcatg tctccaacgt gcttggcaca gaaaatccac
    15481 tggcggaaat gatcacgctt gcgcaccagc atggcgcaaa agtgctggtg gatggcgctc
    15541 aggcggtgat gcatcatccg gtggatgttc aggcgctgga ttgcgacttt tacgtgttct
    15601 ccgggcataa actgtatggc cccaccggaa ttggcattct ttatgtgaaa gaagccttgt
    15661 tgcaggagat gccgccgtgg gaagggggcg gttctatgat cgccaccgtc agcctgagtg
    15721 aaggcactac ctggaccaaa gcaccatggc ggtttgaagc cggtacaccc aataccgggg
    15781 gcatcattgg tcttggcgcg gcgctggagt atgtttcggc gctggggctt aatagcatag
    15841 ccgagtatga acagaatctg atgcattatg cgctatcaca gctggaatct gtaccggatc
    15901 tcactctcta tggccaacaa aacaggcttg gcgttattgc ttttaatctc ggtaaacacc
    15961 acgcctatga tgttggcagt tttctcgata attacggcat tgctgtgcgt accggacatc
    16021 actgcgctat gccattaatg gcctattaca acgtccctgc gatgtgtcgg gcgtcgctgg
    16081 ccatgtataa cacccatgaa gaagtggagc gtctggtgac cggcctgcaa cgtattcacc
    16141 gtctgctggg ataacaggga ggcactatgg ctttattgcc ggataaagaa aaattgctgc
    16201 gtaatttttt acgctgcgcc aactgggaag agaaatatct ctacattatt gagctgggcc
    16261 agcgtctgcc agaattacgc gccgaagacg gaagcccaca aaatagcatt cagggctgcc
    16321 agagtcaggt gtggattgtc atgcgccaga atgcgcaggg aattattgaa ttgcagggcg
    16381 acagcgatgc ggcgattgtg aaagggctta ttgcggtcgt ctttattctc tacgatcaga
    16441 tgacgccgca ggatattgtc aatttcgatg tgcgtccgtg gtttgaaaaa atggcgctca
    16501 cccaacatct caccccatct cgttcacaag gtctggaagc gatgattcgc gcaattcgcg
    16561 ccaaagccgc tgcacttagc taaactaaag ggacagcttt catccggttg gctcacattg
    16621 ccggatggtc aatgtttaca ctgacctgca caggagtgtt ttggtttcag gtgaacataa
    16681 gcaccatccg acgtttcagc gtgagtctcc ggcaaataca ggaggtttac aatgaaacgc
    16741 gcgtctttgc ttacactcac gcttatcggc gcttttagcg ccatccaggc tgcctgggcg
    16801 gttgattatc cgctaccacc aaccggaagc cgactggttg ggcaaaatca aacgtatacg
    16861 gtgcaagaag gggataaaaa ccttcaggct attgcccgac gttttgatac tgcagcaatg
    16921 ttgatccttg aagccaataa cactatcgcc ccggtgccaa aacctggtac gacgataact
    16981 attccttcac aactgttatt acctgatgca ccgcgtcagg ggattatcgt taaccttgca
    17041 gagctgcgcc tttattatta tccgccggga gaaaatattg tgcaggttta tccaataggt
    17101 attggattgc aggggctgga aacgccggtg atggaaacgc gtgttgggca gaaaatccct
    17161 aacccaacct ggacgcctac ggcaggcatt cgtcagcgtt cgctggagcg tggcattaaa
    17221 ttaccgccag tcgttcctgc cggaccaaat aacccgctag gacgttacgc actgcgcctc
    17281 gcgcatggta atggcgaata cctcattcat ggcaccagtg cgccggacag cgttggtttg
    17341 cgcgtcagtt cagggtgtat tcgcatgaat gcaccggata ttaaagcctt gttctccagc
    17401 gtgcggacgg gaacgccggt gaaagtgatc aacgaaccgg tgaaatattc cgtggaacct
    17461 aacgggatgc gttatgttga agtacatcga ccactatcag cagaagaaca gcaaaacgtt
    17521 cagacaatgc catacacact gccagcaggc tttacgcaat ttaaagacaa taaggctgta
    17581 gatcagaagt tagtcgataa agcgttgtat cgtcgggcag ggtatccggt ttcggtgagc
    17641 agtggagcaa ctcccgcagc cagcaatgcg ccttcagtag agtcagcgca gaatggtgaa
    17701 ccagagcaag ggaatatgtt acgcgcgacg cagtagtggt aaatagcaga caaaaaaatg
    17761 gcgcacaatg tgcgccattt ttcacttcac aggtactatt acttgcggta tttagtagcc
    17821 atgttgtcca gacgctggtt agcacgagct gcgtcatctt tagcagcctg aacgtcggaa
    17881 cgcattgcgt tcacgtcgtt gctcagctgg tcaactttag cgttcagagt ctgaacgtca
    17941 gaagacagct gatcgatttt agcgttgctg gagcaacctg ccagcagagt agaacccagg
    18001 attaccgcgc ccagtaccag tttagtagct ttcattatta ataccctcta gattgagtta
    18061 atctccatgt agcgttacaa gtattacaca aagtttttta tgttgagaat atttttttga
    18121 tgggaaggca cttatttttg atcgttcgct caaagaagca tcgaacgctg ggttttagat
    18181 aaaaaaattg agagaaaaca ggatgcttcc atcggattca tcttaaataa agtgagatta
    18241 tatagtgaaa tccgatttga ttttttattg cttctggtta tcgattaaat aaaaaaagcg
    18301 cccatcaggg cgcttcgata tacaaattaa ttcacaaaag caatattaca ggacgtgaac
    18361 agatgcggtg ttagtagtgc cgctcggtac cagtgcacca gaaaccataa ctacgacgtc
    18421 acctttgtgt gccagaccgc tctgcagagc cagttcttta cccagacggt agaaatcatc
    18481 agtagaagtg atctctttaa ccagctgcgg cacaacgcct ttgctcagta ccaactgatg
    18541 agccgttttt tcgttggtgg tcagtgccag gatggtggca tccgggaagt atttacgtac
    18601 tgcgcgagca gatttaccgc cctgggtagc aaccacgatc agcggagcat ccagtttttc
    18661 agcagtttca acggcaccac ggcataccgc ttcggtgatg cgcagtttac ggttgtcatt
    18721 gttgaactcg agacggctgt tcatcacgcg gtcggtacgt tcgcagatgg tcgccatgat
    18781 agaaaccgct tccagcgggt atttaccttt tgcggattca ccagacagca tcactgcgtc
    18841 agtaccgtcg aggatggcgt ttgcaacgtc accggcttct gcgcgagtcg ggcgtgggtt
    18901 tttgatcatg gaatccagca tctgggtcgc agtgataacg actttacgtg cacggataca
    18961 tttttcgatc atcatcttct gggcgaagat aacttcttct accgggattt ctacacccag
    19021 gtcgccacgc gcaaccatga tgccgtcaga ggcttcgagg atttcgtcga agttgttgag
    19081 gccttcctgg ttttcgattt tggagatgat gtggatgttt tcgccgccgt gcgctttcag
    19141 gtgctcacgg atttcgataa cgtcagaacg cttacgaata aaggaagcag caacaaagtc
    19201 tacgcctttt tcgcaaccaa agatcaggtc ctgtttgtct ttttcagcca gtgctggcag
    19261 agcaatggaa acgccaggca ggttcacacc tttgttttcg cccaggtcac cgttgttcag
    19321 cactttacag ataactttgt taccttcaat ggcggtaact tccataccga tcagaccatc
    19381 gtcaaccagt acggtgttgc caacagacag gtcagtagtg aaaccttcat acgttaccgc
    19441 aaccatttcg ctgttgccga taacagattt atcagtggtg aaagtaaagg tctgaccagc
    19501 tttcagagaa acatcgttac cgccttccag tttcatggtg cggatttccg gacctttggt
    19561 atcaagcagg atagcggcgg ttttaccagt tttgctcatc acgttgcgca gattctgaat
    19621 gcgctgaccg tgttctgcat agtcaccatg agagaagttc agacgcataa cgttcatgcc
    19681 agcgtccagc attttagcta acatctcttc agattcggtt ttcggtccga tggtgcaaac
    19741 aattttggtc tttttcatga cagtcttagt ctttaagttg agaaggatgg gagaaacttg
    19801 ctttctgggc gcactgccgg aaagcgaatc cggtattacg aaagtggtgg tgcctcgctc
    19861 taaggatagg tgacatcgaa aaagcgtgca gaggaatgtg tgcttgtgtc tcagcccaac
    19921 gtgagtgtct gtttttgagt cgttgagttc tacagtccaa agtgctgaaa ccattcaaga
    19981 gtcaattggc gcgcattata cgctgaaatt aatccaaaag gaaaacaaaa acagcgtttc
    20041 aaaaaatata caacttgtta gacaataaat tgttatcaaa gatttaacgt tccagggaaa
    20101 aggttacgca aaaacttcgc tgtttgcgca acgtctggtg ccgaagttgt cagtgtgagg
    20161 ataagtgaaa gttcatccgg tgtataaata tatcatcgct caattgctac agttaaaata
    20221 atttatcgcg ttatttgtct gattttgttt gatttttgtt taattggtta gattgatata
    20281 tgctgat
//
LOCUS       DAJTQY010000075        20122 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.075, whole genome shotgun sequence.
ACCESSION   DAJTQY010000075 DAJTQY010000000
VERSION     DAJTQY010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20122
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.075"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            12..278
                     /gene="mliC"
                     /locus_tag="OO349_002774"
                     /pseudo
     CDS             12..278
                     /gene="mliC"
                     /locus_tag="OO349_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310375.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
     gene            337..993
                     /gene="pdxH"
                     /locus_tag="OO349_002775"
     CDS             337..993
                     /gene="pdxH"
                     /locus_tag="OO349_002775"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HCR5867778.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            1122..2396
                     /gene="tyrS"
                     /locus_tag="OO349_002776"
     CDS             1122..2396
                     /gene="tyrS"
                     /locus_tag="OO349_002776"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HCR5867779.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYDVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            2455..3318
                     /gene="pdxY"
                     /locus_tag="OO349_002777"
     CDS             2455..3318
                     /gene="pdxY"
                     /locus_tag="OO349_002777"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HCR5867780.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIEQGPQIVLVKHLARAGYSRDRFEMLLVTADEVWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(3362..3967)
                     /gene="gstA"
                     /locus_tag="OO349_002778"
     CDS             complement(3362..3967)
                     /gene="gstA"
                     /locus_tag="OO349_002778"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HCR5867781.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     SVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLDKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(4073..5575)
                     /gene="dtpA"
                     /locus_tag="OO349_002779"
     CDS             complement(4073..5575)
                     /gene="dtpA"
                     /locus_tag="OO349_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HCR5867782.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPVNY
                     RNLLLTIIGVVALIAVATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKATVA"
     gene            complement(6186..6821)
                     /gene="nth"
                     /locus_tag="OO349_002780"
     CDS             complement(6186..6821)
                     /gene="nth"
                     /locus_tag="OO349_002780"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HCR5867783.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     ISVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(6821..7516)
                     /gene="rsxE"
                     /locus_tag="OO349_002781"
     CDS             complement(6821..7516)
                     /gene="rsxE"
                     /locus_tag="OO349_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HCR5867784.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGTGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRAEAA
                     AERALPNGETGNV"
     gene            complement(7520..8140)
                     /gene="rsxG"
                     /locus_tag="OO349_002782"
     CDS             complement(7520..8140)
                     /gene="rsxG"
                     /locus_tag="OO349_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HCR5867785.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKT
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWLTHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(8144..9202)
                     /gene="rsxD"
                     /locus_tag="OO349_002783"
     CDS             complement(8144..9202)
                     /gene="rsxD"
                     /locus_tag="OO349_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HCR5867786.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLMFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(9203..11329)
                     /gene="rsxC"
                     /locus_tag="OO349_002784"
     CDS             complement(9203..11329)
                     /gene="rsxC"
                     /locus_tag="OO349_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HCR5867787.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQTNGTPLRQVLLAQHFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWVDYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPVDLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKATRLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAEREARKAQARAKQAELEQTNDAATVAEPRKTAVEAAIARS
                     KARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQVDPRKAAVE
                     AAIARAKARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQVDP
                     RKAAVAAAIARVQAKKAAQQKVVNED"
     gene            complement(11322..11900)
                     /gene="rsxB"
                     /locus_tag="OO349_002785"
     CDS             complement(11322..11900)
                     /gene="rsxB"
                     /locus_tag="OO349_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HCR5867788.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRVMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            complement(11900..12481)
                     /gene="rsxA"
                     /locus_tag="OO349_002786"
     CDS             complement(11900..12481)
                     /gene="rsxA"
                     /locus_tag="OO349_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HCR5867789.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLTSICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            complement(12558..12998)
                     /locus_tag="OO349_002787"
     CDS             complement(12558..12998)
                     /locus_tag="OO349_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HCR5867790.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEVGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            complement(13084..13299)
                     /gene="ydgT"
                     /locus_tag="OO349_002788"
     CDS             complement(13084..13299)
                     /gene="ydgT"
                     /locus_tag="OO349_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HCR5867791.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            complement(13572..13697)
                     /gene="blr"
                     /locus_tag="OO349_002789"
     CDS             complement(13572..13697)
                     /gene="blr"
                     /locus_tag="OO349_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HCR5867792.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            13940..14980
                     /locus_tag="OO349_002790"
     CDS             13940..14980
                     /locus_tag="OO349_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR5867793.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKYVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            complement(15016..16017)
                     /gene="add"
                     /locus_tag="OO349_002791"
     CDS             complement(15016..16017)
                     /gene="add"
                     /locus_tag="OO349_002791"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HCR5867794.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLRYVELRFSPG
                     YMAMAHKLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(16121..17293)
                     /gene="malY"
                     /locus_tag="OO349_002792"
     CDS             complement(16121..17293)
                     /gene="malY"
                     /locus_tag="OO349_002792"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HCR5867795.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCNPQNPTGKVWTCDELEIMADLCERHDVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            complement(17303..18895)
                     /gene="malX"
                     /locus_tag="OO349_002793"
     CDS             complement(17303..18895)
                     /gene="malX"
                     /locus_tag="OO349_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HCR5867796.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESVTRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVEDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            19070..20098
                     /gene="malI"
                     /locus_tag="OO349_002794"
     CDS             19070..20098
                     /gene="malI"
                     /locus_tag="OO349_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HCR5867797.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLNGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGITLADRMMQKITHEETHSRNLII
                     PARLIAAK"
BASE COUNT         5130 a         5091 c         5171 g         4730 t
ORIGIN      
        1 aaatataggg gcaaatccag gttgagcgaa tgcaaaccga tacgctggaa taccagtgtg
       61 atgaaaaacc gttgacggtc aaactgaata atccgcgcca ggaagtcagt tttgtttacg
      121 ataatcaact actgcatctc aaacagggcc tttcagcctc tggcgcgcgt tactccgacg
      181 ggatctatgt tttctggtcg aaaggtgagg aagcgactgt ctataaacgc gaccgcatcg
      241 tcttgaataa ctgtcagtta caaaatccac agcgttgaga tttttccagg ggcggcgcac
      301 aatagcgtca cccactgaca atccgtaaag aaaaccatgt ctgataacga cgaattgcag
      361 caaatcgcgc atctgcgccg tgaatacacc aaaggcgggt tacgccgccg cgatcttccc
      421 gccgatccat taaccctttt tgaacgttgg ctctctcagg cttgtgaagc caaactggcg
      481 gaccctaccg cgatggtggt cgctaccgtg gatgaacatg gtcagcctta tcagcgcatc
      541 gttttactca aacattacga cgaaaaaggc atggtgtttt acaccaacct cggtagccgt
      601 aaagcacatc aaattgaaaa taatccgcgc gttagcctgc tgttcccgtg gcataccctt
      661 gaacgccagg tgatggtgat cggtaaagca gaacgacttt cgactctcga agtgatgaaa
      721 tattttcata gccgcccgcg tgatagccag attggtgcat gggtttcgaa gcagtccagt
      781 cgcatttctg cccgcggtat ccttgaaagt aaattcctgg agctgaagca gaagtttcaa
      841 cagggcgaag tgccattgcc gagcttttgg ggcggttttc gcgtcagcct tgaacagatt
      901 gagttctggc agggcggtga gcatcgcctg catgaccgct ttttgtacca gcgtgaaaat
      961 gatgcgtgga agattgatcg tcttgcaccc tgaaaagatg caaaaatctt gctttaatcg
     1021 ctggtactcc tgattctggc actttattct atgtctcttt cgcatctggc gaaaagtcgt
     1081 gtaccggcaa aggtgcagtc gttatataca tggagatttt gatggcaagc agtaacttga
     1141 ttaaacaatt gcaagagcgg gggctggtag cccaggtgac ggacgaggaa gcgttagcag
     1201 agcgactggc gcaaggcccg atcgcgctct attgcggctt cgatcctacc gctgacagct
     1261 tgcatttggg gcatcttgtt ccattgttat gcctgaaacg cttccagcag gcgggccaca
     1321 agccggttgc gctggtaggc ggcgcgacgg gtctgattgg cgacccgagc ttcaaagctg
     1381 ccgagcgtaa gctgaacacc gaagaaactg ttcaggagtg ggtggacaaa atccgtaagc
     1441 aggttgcccc gttcctcgat ttcgactgtg gagaaaactc tgctatcgcg gcgaacaact
     1501 atgactggtt cggcaatatg aatgtgctga ccttcctgcg cgatattggc aaacacttct
     1561 ccgttaacca gatgatcaac aaagaagcgg ttaagcagcg tctcaaccgt gaagatcagg
     1621 ggatttcgtt cactgagttt tcctacaacc tgttgcaggg ttatgacttc gcctgtctga
     1681 acaaacagta cgatgtggtg ctgcaaattg gtggttctga ccagtggggt aacatcactt
     1741 ctggtatcga cctgacccgt cgtctgcatc agaatcaggt gtttggcctg accgttccgc
     1801 tgatcaccaa agcagacggc accaaatttg gtaaaactga aggcggcgca gtctggttgg
     1861 atccgaagaa aaccagcccg tacaaattct accagttctg gatcaacact gcggatgccg
     1921 acgtttaccg cttcctgaag ttcttcacct ttatgagcat tgaagagatc aacgccctag
     1981 aagaagaaga taaaaacagc ggtaaagcac cgcgcgccca gtatgtactg gcggagcagg
     2041 tgactcgtct ggttcacggt gaagaaggtt tacaggcggc aaaacgtatt accgaatgcc
     2101 tgttcagcgg ttctttgagt gcgctgagtg aagcggactt cgaacagctg gcgcaggacg
     2161 gcgtaccgat ggttgagatg gaaaagggcg cagacctgat gcaggcgctg gtcgattctg
     2221 aactgcaacc ttcccgtggt caggcgcgta aaactatcgc ctccaatgcc atcaccatta
     2281 acggtgaaaa acagtccgat cctgaatact tctttaaaga agaagatcgt ctgtttggtc
     2341 gttttacctt actgcgtcgc ggcaaaaaga attactgtct gatttgctgg aaataatgca
     2401 ttaagtggaa agggggagtg agaaatcact ccccctggtt tttatacagg gaacatgatg
     2461 aaaaatattc tcgctatcca gtctcacgtt gtttatggtc atgcgggtaa cagtgcggca
     2521 gagtttccga tgcgccgcct gggcgcgaac gtctggccgc tgaacaccgt tcaattttct
     2581 aatcacaccc aatacggcaa atggactggc tgcgtgatgc cgcccagcca tttaaccgaa
     2641 attgtgcaag gcattgccgc cattgataaa ttacacacct gtgatgccgt attaagtggc
     2701 tatctgggat cggcggagca gggtgaacat atcctcggta tcgtccgtca ggtgaaagca
     2761 gcgaatccgc aggcgaaata tttttgcgat ccggtaatgg gtcatccgga aaaaggctgt
     2821 atcgttgcac cgggtgtcgc agagtttcat gtgcggcacg gtttgcctgc cagcgatatc
     2881 attgcgccga atctggttga gctggaaata ctctgtgagc atgcggtaaa taacgtcgaa
     2941 gaagcggttc tggcagcgcg cgaactcatt gagcaagggc cacaaattgt gttggttaaa
     3001 cacctggcgc gagctggcta cagccgtgac cgttttgaaa tgctgctggt caccgccgat
     3061 gaagtctggc atatcagccg tccactggtg gattttggta tgcgccagcc ggtaggtgtt
     3121 ggtgatgtga cgagcggttt actgctggtg aaactgcttc agggggcaac gctgcaggag
     3181 gccctggaac atgtgaccgc tgcagtctac gaaatcatgg tgaccaccaa agcaatgcag
     3241 gaatatgagc tgcaagtggt ggctgctcag gatcgtattg ccaaaccaga acattacttc
     3301 agcgcaacaa agctctgaaa tatctcattt cggcccacaa cgacctgtgg gccgatggct
     3361 attactttaa gccttccgct gacagcgcgt cttgtacttc cggacgttca gccatacgtt
     3421 gcataaatgc cgcaatgtgc tctaaccctt ccagattcag tttcaccgcg tatgcccagc
     3481 gcagaaccgt aaacagatag gcatcagcaa ttgtaaatct ttgcccgcag atccagtgct
     3541 catccttcag tgcctcgttc acatattgca gcttcttatc cagctgcgcg cgaactgtcg
     3601 gtttgtactc ttccggtgta tctgggcgaa acagaggtgt gaaaccttta tgcagctcgg
     3661 tggcgatgta attcagccat tcgatggttt tatagcggga aatactgttt accggtgcca
     3721 gcaactggcg gtcggggacg ctgtcggcaa gatactgcat aatcgcaacg ccttccgtca
     3781 gcaaagtacc atcgtccagc agcaatgcag gcacctgccc cttagggtta acggaaaagt
     3841 aatcgtcacc gttttcgaga cgttttttca ttaaatccac actgacgagc gtaaaatcct
     3901 ttccgctctc acgcagggtg atatgggaag cgagagagca ggcgcccggt ttgtagaaca
     3961 atttcatcgg taactccttt tgtctgtggt ttaaagtatg gtagtgcgtt gctgaacaaa
     4021 aaaaaagccg ctaacgaaaa gttagcggct tgtaaaagag tttccctgaa ttttacgcta
     4081 cagtagcttt cgccgctttg tctgcagcgt catcctgcgt catgcggtgc agtttaggcg
     4141 cggtcagcag catcagtact gcaataacgg cagtagcgac accaatctgc aagaatactc
     4201 gaccatagac ttccagtgac atcagcgggt cggtaacgtt atccggcaca gccatcatac
     4261 ccgcaacata accaccaatc aggtttgcac cggcagtggt caggaaccag ctacccataa
     4321 tgaagcccat cagacgctgc ggaacgagtt gtgcaaccat tgccagaccc agaccagaga
     4381 tcatcagttc cccgatgctc tgcaggccat agcttgcgac cagccagctg acagacacga
     4441 tacctgcgtc agacgcgaat ttcgcaccca gcggcagaat caggaacgca ccagaacaca
     4501 tcaccatgcc gattgcaaac ttggttggca tcggcagggt atcacccatc ttgttataga
     4561 tagcggccag aatcggacta ccgatgatga tccagaacgg gttcagtgcc tgatactgct
     4621 caggttctac ggccagaccc agaatggagt gctcaacgtt acgaatcgca aagaagttca
     4681 gtgacgttgg catctggctg tacagcacga agaagatgat tgcttcgagc atcaggatga
     4741 aggcaacgat cattttacga cgcgcagcac ctttcatcgc gaaagcttct ttaccgaaga
     4801 taaccacaat accgaaggca acaacgccca gcgccatacg cgcaacttcc tgattgtgca
     4861 gtagccaggt ggcaacagcg atcagcgcca caacaccaat aatggtcagc aacaggttac
     4921 ggtagttgac tggttcaaag tctggttttg aaccgtactg tttaacccag cgttggcaga
     4981 aggcgaagtt aacgatagtg atcagcaggc ctacaacgct caacgcaaac gcaacactcc
     5041 agccgtattt cgcggccagc cacggggtgg ctatcataga gaagaaagag ccgatgttga
     5101 cggacatgta gtacatggtg aatgcaccgt ccagacgtgg gtcgtttttc tcatagcatg
     5161 tagaaagcag agaagacggg ttagctttaa acaggccgtt accgaccgca atagccgcca
     5221 tacccatata aacaataccg gcgtcgtgac cagaccaggc aaccagagca taaccaatcg
     5281 ccagcacaat agcgccgagc ataattacgc gtttagtacc cagtaccttg tcacctaacc
     5341 agccgccgat agcgaccaga ccataaacca gggcactaaa ggaagagaaa agggtgattg
     5401 aatccgcttc agacataccc agttgtttaa ccaggtaaac agccataatt ccttgtaggc
     5461 cgtaataacc aaaacgttcc cataactcaa tcgagaagat gagatagaac gccttcggtt
     5521 gtttgaaagc gttcaaactg acgctttcag ttggtttttg gtttgcagtg gacactttta
     5581 cctctttttt tacatcccat attaacgggg gtgctttctc gcgttatgtc taaaaaacat
     5641 acgcgcaatt gttatagtgg gaggggaaac ggcaggtaat gttcactatc ttgcactttc
     5701 agacaagtcc tttgtaataa tctgttacat ataactgagg cggggaattc aaccattccg
     5761 ggaaatgtta tccagcgtta aatttttcaa aaaacgtaac ggcagatttt ttcactgcat
     5821 tgagaggggg atgatggctt ggcggcgata tgttctgcta tttagcgttt ttcctagtga
     5881 gatagtccag tttctgaaaa atagccagtg taatgttttg caggtcaata tttgttgcgt
     5941 gtgtaaattt aaatcatttt aacctgattc ttgcaatgtt tttacattgc cacagcatcg
     6001 tgatcttgat cacgaatgaa tagaaaattt cgctattaaa aaaacaatta acctgcattt
     6061 ttggttatcc gcaaacggat tacctgatga taacgctaag caatggcatc attgacgtta
     6121 aaaagatgtt ttaaaagagg ataaagaaag gttatcaatg gggtaatcgg tgttacccct
     6181 tttcttcaga tgtcaacttt ctctttgtat tcacaaagat cttcaataat acaagagcca
     6241 cagcggggct tgcgggcaat gcaggtataa cgcccgtgca ggatcaacca atggtggcag
     6301 tcgactttaa actctgccgg aaccactttc agtagctttt cttctacctg ttcgacgttt
     6361 ttccccggcg caaattgagt acgattacaa acgcggaaaa tgtgcgtgtc gacagcaata
     6421 gtcggccagc cgaatgcagt gtttaatacg acgttggctg ttttacgacc tacgccgggc
     6481 agggcttcaa gcgcagcacg atcttccgga acctcgccat tatgttgttc cagtaatata
     6541 cggcaggttt tgatgatatt ttctgctttg ctgttataaa ggccaatcgt tttgatatag
     6601 gttttcaccc cttcaacgcc tagttcaagc atcgctgcag gcgtattcgc caccgggtag
     6661 agtttcgccg tcgccttatt aacactgata tcggtcgcct gagcggaaag cagtacggca
     6721 atcagcaatt caaaaggcga actgaaatta agctcggtgg tgggatgagg attgttctca
     6781 cgcaggcgag tgaggatctc cagacgtttt gctttgttca tcagacattc cctgtttcac
     6841 cgtttggcaa tgcacgttcg gcagctgctt cagcacggcg ctttttcatt ctttcatcaa
     6901 tcaggtattt tcctgccagc atcagtccca agccaataaa tgcacctggt ggcagcatcg
     6961 ccagcaggaa aggggagtcg gtgtggaaaa tctccacgcg taatactttt gcccagctac
     7021 ctaacagcgc atctgcaccg tcaaacaacg tgccattgcc gataatttcg cgtagtgaac
     7081 ccagcacgaa catggcgcag gttgcgcccg taccaattga aaagccgtcc agtgccgaaa
     7141 gcgccggacc ttttttggcg gcgaaggctt cagcgcggcc cacaacgata cagttagtga
     7201 caatcagcgg aataaaaatc cctaatgatt gatacaggcc aaaggcgtag gcgttgatca
     7261 gcatctgtac agcgctgacc accgaggcga tgatcatcac gtaaatggga atgcggatct
     7321 cggctggcgt ccagtgacgc agcgtcgaaa tggtcaggtt ggtcagcgtc agtaccagcg
     7381 tagtcgcaag tcctaaaccc agagcgttag tggcagtgga tgtgaccgcc aatagcggac
     7441 aaaggccgag cagttggacc agtgcagagt tgtttttcca caacccctga acaataacgt
     7501 ctttaatttc gctcacgctt tattctccac aggcaggaag ttgagaaagt tgtgccggta
     7561 acgtctgagc gtacaatccg gcgcgtttta ccgcattaac caccgcgcgg ggagtaatcg
     7621 tcgcgccggt gaactggtcg aaatcaccac catctttctt caccgcccag tgcgcatcat
     7681 ccgcaccact gatttttttt cccgcaaaat gggtgagcca gtcagaaagg cgcaattcga
     7741 ttttatcgcc aagccctggc gtttcgtggt gctctgtcac gcgcgtgcca agtaccgtgc
     7801 cattaaaatc ggctcccacc agcagctgaa tcgcaccgga atagccatct ggcgcggttg
     7861 cttccagaac ggcggctacc ggtttgtcat cctgtttggc gatgtaaacc cgatgctcac
     7921 ctttacctaa ctctggcgca gttaccagat agcaactctg tgcgagcgca ttgttatagc
     7981 gttcggctgg cagcacctga tcaaataacg tcttttgttg cagactggcc tgttcagcaa
     8041 ttgtcgtttt ggtcatctgg ttgatggccg cagttaaccc tgtagaaccc gctgcaaata
     8101 gtgccagtgt aatgccgtgt tttcggatag ttttcagcat ggtttaccct ttgcgatggc
     8161 cgtagacgcg cggacgcgtg tagtaatcga tcagaggaac cgtgatgttc gccagcagga
     8221 cggcaaaagc cacgccgtca ggatagccgc cgaaactgcg gatcaaccag actaataagc
     8281 ccgccagcgc gccgaacatc agccgaccac gattggtcgt agaagcagta actgggtcag
     8341 tcaaaataaa gaatgcgccg agcatagtcg caccagacag cagatgaatt tgcggtgctg
     8401 ccagtgtttc tggtgagaac aaccagccca acgttgcgca taacgccagc gttactaaga
     8461 agctgagggg aatatgccag cgaatcgctt tctgccatag caaccacacg ccaccagcca
     8521 gccaggcgag atttacccat tgccaaccag cgcccgccag aataccgctg tagatcggat
     8581 attgcataat ctgttcaacc gaatgacctg cgcggacaga ggttttgaat gtatccagcg
     8641 gtgtcgcctg actaatgcca tcaataccta agcgtagcgt attcatatca ccaccactgg
     8701 cggtatgacc gctgaaaata acctgtatgg cgtcgataaa accagggatg ttgaccgcaa
     8761 tttcatgtgg cggtaaccag ctggtcatct gcaccgggaa ggagatcagt aagaccacat
     8821 aaccaatcat tgccggatta aacgggtttt gccccagacc gccatacaac tgtttagcga
     8881 tgatcaccgc aaacaccgta cccagtacga ccatccacca tggcgctagg gggggaatac
     8941 ttaccgccag caataagcct gtcagcaatg ctgagttatc tttcaacgtt gcggctaccg
     9001 actgcttgcg tagtttgagt acgagagctt cggctaacag agcactgacc gacgccaaca
     9061 ggatctgaac gagagtaccc caaccaaaaa accacagttg cgctgcgatt cctggcacgg
     9121 ctgcgagcaa caccaacagc ataatgcgcg atgtctggcg ctggttatgg gtataagggg
     9181 agctagctat tctgaatacc atttagtcct cgtttacaac cttttgctgg gcggcttttt
     9241 tggcctgaac gcgggcaata gccgcggcaa ctgcggcttt gcgcggatcg acctgttctt
     9301 ctggttccgc attagcctgt tgctgttcca gcttgcgtgc tttggcgcgg gcaatagcgg
     9361 cttcgacggc agctttgcgc ggatcaacct gttcttctgg ctccgcattg gcctgttgct
     9421 gttccagctt gcgtgctttg gcgcgggcaa tagcggcttc gacggcggct ttgcgcggat
     9481 cgacctgttc ttctggttcc gcattagcct gttgctgttc cagcttgcgc gctttggcac
     9541 gggcaatagc ggcttcgacg gcggctttgc gcggatcgac ctgttcttct ggttccgcat
     9601 tagcctgttg ctgttccagc ttgcgcgctt tggagcgggc gatagctgct tcaacggcag
     9661 ttttacgtgg ttcagcaacg gttgctgcgt cgttagtttg ctccagttct gcctgtttcg
     9721 ctctggcttg cgctttacgg gcttcccgtt ctgcaataat tgcactgtta tccgggcgtt
     9781 cgcccgcttt aatcacaata ggctgtgtag cctgggcctg tttctctttc acccgcgcca
     9841 gagcggcagc aatcgcatct ttatctttgg ctgcaggttg aacggctgcg ctcttatgtc
     9901 gttcaaggcg agtcgctttt tcgcgctcca gacgagcctg gcgcgcttcg aaacgcgctt
     9961 tggcttctgc ggcgcgcttt tcttcctgac gaatagccgc aatttcagct ttttcctgac
    10021 ggaaatattg caccaaggga atattgctcg ggcaaaccca cgcgcaagcc ccacattcaa
    10081 tgcagtcggc aatgttatgc gtggtagctt tatcgtgttg ctgacctttg ctgaaccagt
    10141 acaactgttg cggcaaaaga tccacagggc aggcgtcagc acaggcgcta caccggatgc
    10201 agctttgttc ttcctgtggt tcgccaagtt cattggcaga gggagccaac agacagttgg
    10261 taatctttac gaccgggaca tccagccatg gcaaggtaaa gcccattagc gggccaccca
    10321 taatcaccat ttgatcggca gaggggcaga atccggcatc attcaataaa tgacgcactg
    10381 gcgtccccag ccgtgcccag acgttgcccg ggcgagcgat tgcttcgcca gtcagggtta
    10441 caacacgctc ggtaatcggc tcgccatcaa taacggcacg tttcactgca taagcagtgc
    10501 cgacgttttg cattaatacg ccgatatcgg atgaacgccc gccatgtgga acctgcttcc
    10561 cggtcagaat gtaggttaat tgtttagcac cgccagaagg atatttggtt ggaatcaccc
    10621 gcagagaaat atcgttagag tccgccagca ccgcgcgcag catggaaatc gcctgcggtt
    10681 tgttatcttc aatgccgata agaatttcgc gtggctgcag aatatgcgca agaatgcgaa
    10741 taccctctac gacctgagcc gcgcaatcct gcatcaaacg gtcatcggcg gtaatgtacg
    10801 gctcgcactc agcagcgttg ataatcaacg tttcaatctt atctccacca ccctgcaatt
    10861 taacgcctgt cgggaatccg gcaccgccca gcccggcaac accaaactga tgtatgcgct
    10921 cgattaactc ttcgcgactg cgagagcgat aatcgaccca gccgtcgcgc gggatccagc
    10981 agtcttcacc atcggcatca ataatcacgc ttaattcagc taaagctgaa ggatgagccg
    11041 tagagtgggg cgcaatagcc gtaacggtac ccgaggtggg cgcgtgaaca ggcagcattt
    11101 tgccgcgacc acgggtaagc ggctggccgc gcaatacttt atcgccgacg ctaacgcaca
    11161 actcaccttc agcgccaata tgctgtttca gtggaataac aaaatgctgc gccagtagta
    11221 cctggcgcag gggggtaccg ttggtctggg ttttcatctc cggtggatgg atgccgccgt
    11281 tgaaatccca gattttattt tttctgaatg cagagaataa cttaagcatg gtgttccacg
    11341 ggaatgatac gcacgggaat ggtgttcaga tcccatttcc aggagtcagg tgtttctgcg
    11401 accggttgca acgagatgca gtgcgtcggg cacggatcaa cacataaatt gcagcccgta
    11461 cagagatcac tcattaccgt atgcatgaca cgggtagcgc caacgatggc gtctaccgga
    11521 caagcctgaa tacatttagt gcagccaata cagttatttt catcaataac cgccaccatc
    11581 cgcgcaggcg ttagctcttg cgcttcgcca tccagcggct gcggctcgac attaagcaac
    11641 tcggcaattt ttagcatcac agcttcgcca cctggggcgc aacggttgat tttttcaccg
    11701 ttacagctga tggcttccgc gtaggggcga cagccgggat aaccgcactg accacactgg
    11761 ctctgcggta agatttcgtc aattttctca acgaccggat cgtcttccac cgcaaaacgg
    11821 cgggaggcat aacccagaat ggcgccaaac gccaggccca gcaggctcac ggcggcaacg
    11881 gcaatccaga tagcattcat tacaacttca ccaaaccact aaagcccata aaggccagag
    11941 acataagacc tgcggtaatt aacgcaatgg cattaccgcg aaaaggtgcc gggacatcag
    12001 ccacagcaag gcgttcgcgg atggcggcga agagcaccat caccagcgag aaaccgacag
    12061 cggcggaaaa accgtacagc gccgactgca agaaattgtg cccgagattg atattcagca
    12121 acgccacgcc gagcactgca cagttggtgg tgataagcgg caaaaaaatc cccagcagac
    12181 ggtaaagcac cgggctggtt ttgcgcacca ccatctcggt gaactgcacg accacagcaa
    12241 tcaccagaat aaatgccagg gtgcgcaggt aaatcagatt aagtgggatc aaaatccacg
    12301 tatcgataag ccaggcgcaa atagacgtca gcgtcatcac aaacgttgtt gccagcccca
    12361 tgcccatcgc ggtttccagt tttttggaaa cccccataaa cggacagaga ccgagaaact
    12421 tgaccagtac aaagttattg accagtacag ttccgacaaa cagtaacagg tagtcagtca
    12481 tttttcggcc tgaaataaaa aagccgccta ttatccgtta atccagagca ggcgacaaca
    12541 ggttaactgt aaggttatta cggattcaca aacgtcgctt ttacccgcga cgagcgtttg
    12601 aaatagggga cgatcaatgc ggttgccaac aaagtaaaca gcaactggcg aacggctatg
    12661 ccaacttcca cgggtgaaaa agcgaatgct tttactgcca gcaatacgga aataagcagc
    12721 cagataatat agtgtttagg aacacaacgc cgacgtttaa agaaagcgat ggtcaaccac
    12781 agtgtgtaat accacatggc gatagcggta ataaaagaaa cgccccataa gatttgtgtc
    12841 gtcagtgctt gtccgccgag cgtttgaaat gtttgcggag aagataacgc agcggtatac
    12901 agcaacaacg ccagcgtcgt actcaacaga gcgactaaaa gccaggccaa cgggccaagc
    12961 aaccagcctc caatacgttg tggcgttgtg gtggtcatag gttctccaga atagcgacgc
    13021 aaacaggcaa atctaatttt aggggcgtta ttataaggct tttataaccg atcgctactt
    13081 tctttattgg acatagtgcc agacggactt cggtacctgg ccgaggtcaa acaaacgccc
    13141 gccagaaacc agctctgcgc gacggtgatc ggcagcacga tacatattga tcagttcctg
    13201 atcgtccgtc agggtgtaat taagatggtc gtagagtttt tccagacttt cgagtgaact
    13261 gattttgcga aattttaata agtagtcctg aacagtcata aaaggtccat aaaataatta
    13321 ataccaaagg ggtaggtatt attagtgatg ataaaatttc accaaaagtg aacgcttgtg
    13381 ttattacaat aataagaatg cgctgatggc aaatattcag ggatgaaaaa ctgctctgaa
    13441 aagagtaacg cgctttacta tccctgacaa tcaccaacaa catcgaacaa gataataaat
    13501 tcctggttta atatccgaca agtgaaaaca tgcacccgga cgggcagcat gtcgctccac
    13561 aagtgcagag cttacttgtg ttgtaccgaa gcactctgtt caggtggctg atagttgtca
    13621 atgtgactcg ccacgccaag aagaatgact gaaacgacaa gaacgatcca acctgttaat
    13681 tcaataagac gattcattac agcccacatc tctcttgatt gatccattaa cttcaggggg
    13741 taaatgttac ttagcaataa tagctccagc aagattttta ctgaggtttt tgcgatattt
    13801 agcttttgtc gttggaaaat tcgctatttt tttgacttaa gttaaacaac atcccttatt
    13861 gctggcaggt tattaaactg ttgagcgtgg gtaaaggata gtgtcaaata gccatcatac
    13921 ttcaatgaga ggcaatgaca tgagcgacaa catccgtgtt gggttgattg ggtatggtta
    13981 tgcgagcaaa accttccatg cgcccctgat tgcgggcacg cccgggctgg aactggcggt
    14041 aatctccagc agtgatgaaa caaaagtaaa agccgactgg ccaacggtta cggttgtctc
    14101 tgagccgaag catctgttta acgatcccaa catagacctg attgtcattc ctacacccaa
    14161 cgatacccat ttcccgttag ccaaagcggc gcttgaggcg ggtaaatatg tggtcgttga
    14221 taaacccttt accgtgacac tgtcacaagc gcgagagctg gatgcgctgg caaaaagcct
    14281 ggggcgtgtg ctgtctgtat tccataaccg tcgttgggat agcgatttct tgacgctaaa
    14341 aggtttgctc gcggaaggcg tgctgggtga agttgcttac tttgagtctc attttgaccg
    14401 cttccgtccg caggtgcgcg atcgttggcg tgaacagggc ggcccaggca gcggtatctg
    14461 gtacgattta gcaccgcatc ttcttgatca ggccattacg ctatttggtt taccggtcag
    14521 catgacggta gatttggcac agttacggcc cggagcgcag tcgaccgatt atttccacgc
    14581 catcttgtcc tatccacagc ggcgagtcat tttacacggt accatgctgg cagccgctga
    14641 gtcagcacgg tatatcgtgc atggatcccg aggcagttat gtgaaatatg gcctcgatcc
    14701 acaggaagaa cgtctgaaaa atggcgagcg tctgccgcag gaagactggg gctacgatat
    14761 gcgtgatggc gtacttaccc gcgtggaagg tgaggaacgt gtcgaagaaa cgctgttgac
    14821 ggtgcctggg aattatccgg cttactatgc ggcgattcgt gatgcgttaa atggcgatgg
    14881 tgaaaatccg gttccggcaa gccaggcaat ccaggtaatg gagttgattg agctgggcat
    14941 cgaatccgcc aaacatcgcg cgacgttgtg ccttgcgtga aaaatggatg gtgcaatttg
    15001 caccatccat ttttgttact tcgcggcgac tttttctcgc agtgcgcgtt tttcctctgc
    15061 gctgaggaaa gccatttcca gaccattaat ctgtgcctgg cggatttgct cacgggataa
    15121 cccagcagct ggcgcggcaa cggtatattc gtgaatgata tccactccct gtacgccggg
    15181 atcgtcagtg ttaatgctgg cacgaatgcc atgctcaagg aacgttttca gcggatgtgc
    15241 agccagctct gctacggtgc tggtctgaat attggaggtc agacaggatt caataccaat
    15301 ttgttgctcg gcgagaaaat ccatcagcgc ccgatcttca atggctttta cgccatgtcc
    15361 aatacgctcc gcacccagtt cacgaatcgc ctgccagatg ctttccggcc cggcagcttc
    15421 gcctgcatgg acggtaatat gccagcccgc atcacgcgcg cggttgaagt gagaaaggaa
    15481 cagacttccc gggaatccaa gttcatcacc agctaaatca agtgcggtaa tctggtcacg
    15541 gtgggctaaa aaggcctcca gctcttgctg acaggcggct tcgccgaagg ttcggctcat
    15601 aatgccgata agcttcgcct gcacaccaaa ggtgcggcaa ccttcacgta cgccatcgat
    15661 caccgcttcg acaacacccg ctacaggcag cttatgtgcc attgccatgt agcctggtga
    15721 aaaacgcagc tcgacatagc gcaggccgtt acgggctgca tcttcaatgt tttcaaatgc
    15781 cacgcggcgg caggcatcaa gagaggcgag aactttaacg ccccagtcaa gtttggtcag
    15841 aaagctcacc agatcgggtt cgttggcaat gacctgaacg tggggaatca gtgtttcaag
    15901 ggattgtgca ggaagcgaga tattatactg gcggccaagt tcaagaatgg tctggggacg
    15961 aatgttgcca tcaaggtggc gatggatatc agttaatggc agggtggtat caatcatggt
    16021 cgcactcttt ttctggatat aaaagtgcgc accattataa aaacaaagca gggtaaaaat
    16081 ccacgattgc gcaacgattt tttacgttgc gcaattgggg ttagcgaaca gcgcggatgg
    16141 cgtttattaa tccagccaca cctttttcca gtttcgaacg tgggcagccg gcattgagac
    16201 ggacaaaacc acgaccttct tcaccatagg tataccccgg catgatcgcg actttttctt
    16261 gctcgataag cgctttttgc aacgcgttgt cgtcaatatt caacggacgt agatcaagcc
    16321 aggccagata agtggattgc gggatctgcc agttgagttc aggaaacgcg gcgttcattt
    16381 tatctgagat atacgtcagg ttatctttca gatagacgcg taaggcatcc agccacggtg
    16441 caccttgctg ataggcggcg atatgggcag ttaacgccag taccgaaggg gaagaaagcc
    16501 catcacgtcc tttcagtgcc gataaatagg catcacggct gctgctattt tctataatcc
    16561 cgtaagcacc ggtcagcgcg ggaatattga aacttttcga tcccgatgtc agcaacgccc
    16621 agtctccccg cgccacatta ctccagggaa tatgcggctg ctcgccccag accatatcca
    16681 tatggatttc atcggaaata acccgcacat catgacgctc gcacaggtca gccatgatct
    16741 ccagctcatc gcacgtccac actttcccgg taggattctg tgggttacac aggagcataa
    16801 ttttacattc tggtttcgcc aaaacggctt ccagcttgcc catatcgcaa aaccagccgt
    16861 cagcctgctt ctctaaagca acgggcatta ctgtgcgctg gttaccttca atggccttgt
    16921 aaaatgcgtc ataggcgggc gtgtggatca ccacgccttc acctgtttca gaccactgac
    16981 gaatcagttc tgaaaccata tagatgacag aagggccata caccaccgtc tgcgtatcaa
    17041 ttgcggtgta atgctgggtg gaaaaccagt gggcaatagc cgcgagaaac tcatcgtttt
    17101 tccagcggct gtagccaaat acgccgtgca tcaggcgctg attcagcgcc tcgataatgc
    17161 agggggcagt ggcaaaatcc atgtctgaaa tcgtgaacgg taacaggtca gcagtgccga
    17221 aacggtcagc gacataatcc cactgtgtac accatgtgcc atgacgatcc acgacctttg
    17281 aaaaatcgaa catatcttat ccttatgcct ggacagtatg catcagaccg gccatttcat
    17341 cttttactga ctgaacttgt ggcccgataa caacctgcag gttatgttga ttaagttgta
    17401 ctacgccaat tgcccgattg tccttcagtg cctgcacatt aacaagcgac atatcttcca
    17461 cagacaaacg cagacgggta atgcagttat cgaggctgac aatattgtcg gcaccgccta
    17521 atgcttcgag gattgcagga acgttgtaac ctgatttacc cggcgcaccg gcaacggctt
    17581 tttcgattga gctggcaact tcgctatcgc gccccggggt tttcagattg aagcgggtga
    17641 tagcgaaacg gaagatgacg tagtaaacga caaaccagat tgccgccacc actggcacca
    17701 tgtaccactt ggttgacaga ccatgcaaaa taccgaacac cacgaagtcg atgatattgc
    17761 cgtcggtatt accgatggtg acgccgagca cagacatgac ggtgaagccg aggccggtta
    17821 acagcgcgtg gatgacatac agaactggcg ctacgaacag gaacaggaat tccagcggtt
    17881 cggtagtgcc gccaacgacg caggcgatca ggccagaaat cagcagacct ttaattttat
    17941 ggcgattttc cgggcgcgcg cagtgataca tagctaacgc tgcacctggt agaccgccga
    18001 gaaacgcagg cattttacct tgcgaaagga aacgcgtgac gctttcagaa aaaccgtgag
    18061 tggtcgggca actcaattgc gcctggaaga tggtcagtgc gccgctgacg gtttgaccgc
    18121 agacctcctg cgtgccgcct gcgtcggtaa agcgaattaa tgccaccaga atgtgatgca
    18181 gaccaaacgg caacagcaga cgttcaccgg taccaaacag catcggtccg aaatcacccg
    18241 cgctgtttat catatggccc aagccgctaa tacccatggc gaaaatcggc cagactaatg
    18301 gaatcaccag gccgacaagg cccatcacca gcgaggagat aattggtacg aagcgcgtac
    18361 cgccgaagaa tgccagcgca tccggcaggc ggatattatg gaaacgctca tgcagcatcc
    18421 agacgataat accggcgatc accgcaccga ggatcccggt atcgatcgac tggatcccaa
    18481 gaatgctctg gatgttattg gctttcagaa cggcggcatc tgtggttggc agaatgcctt
    18541 tattggtcaa ccagaagttt accgcgaggt tcattaccgc ataaccgacg aagccagcga
    18601 atgccgctac gcctttattt tcgcgtgcca ggcccagcgg gatggcgata cagaacatga
    18661 caggcaggaa actaaaagca aacgagccaa tcttactcat ccaggtaaag atagcctgca
    18721 acacggggtt gcccaggacc gggatcaggg ttatgacatc atggctgcta agagaactac
    18781 caatgccgag cataatgccg cagaacgaca ataatgccac gggtaacatg aaggttttgc
    18841 ctaactgctg gaagaactcc cacagcgtga ctttcggtgc tgttttcgcc gtcataaaac
    18901 gactcctcgt agagaacaat ctggattcag taaattgcgc gagaagataa aacgttttat
    18961 caaattttag tgagtcataa atcacattac gcaacgataa tagcgggtat aagataaata
    19021 aaaggtaaaa cgttttatct gtcacataat cagggagtag gtcatctgca tggctaccgc
    19081 caaaaaaata accattcatg atgttgcgct ggctgcgggc gtgtcggtaa gtaccgtttc
    19141 gctggtgctt aatggcaaag ggcgaatctc taccgccaca ggagaacgcg ttaacgccgc
    19201 cattgaagag ctgggatttg tgcgcaatcg acaggcgtcg gcgctgcgcg gcgggcaaag
    19261 cggcgtcatt ggtttgatcg tccgtgattt atctgcgccg ttttacgccg aattgacggc
    19321 cggattgacg gaagctctgg aagcacaggg acggatggtt tttttgcttc acggcggtaa
    19381 agacggcgag cagctggcac agcggttttc actgttactg aatcagggtg tcgatggtgt
    19441 ggtaattgcc ggggctgcag gaagcagcga tgacctgcgg cggatggcag aagaaaaagc
    19501 tatcccggtg attttcgctt cccgtgccag ttatcttgat gatgttgata cggttcgccc
    19561 ggacaacatg caggctgcac agttgttgac ggagcatctc attcgcaatg ggcatcagcg
    19621 gatcgcctgg ctgggagggc aaagttcctc attaacccgt gcagaacggg tggggggcta
    19681 ttgtgcaact ctactaaaat ttggcctgcc gtttcacagc gattgggtgt tggagtgcac
    19741 ttccagccag aagcaagccg cggaagctat cacggcgctt ttacgtcata acccgaccat
    19801 cagtgccgtg gtttgctata acgaaactat tgcgatgggg gcatggtttg gtttgctgaa
    19861 agcagggcgg caaagcgggg aaagcggagt cgatcgttac tttgagcaac aggtttcgct
    19921 ggcggcattt accgatgcga caccaaccac acttgatgat atacctgtta cctgggccag
    19981 cacgccagcg cgggaacttg gtatcacact tgcggatcgc atgatgcaaa aaatcaccca
    20041 tgaagagacg cattcacgca atcttattat tcccgcccgg ctcattgcgg cgaaataatc
    20101 ctctctttat ctgctatact tg
//
LOCUS       DAJTQY010000076        19414 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.076, whole genome shotgun sequence.
ACCESSION   DAJTQY010000076 DAJTQY010000000
VERSION     DAJTQY010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19414
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.076"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            111..2201
                     /gene="pbpC"
                     /locus_tag="OO349_002795"
                     /pseudo
     CDS             111..2201
                     /gene="pbpC"
                     /locus_tag="OO349_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; incomplete; partial
                     in the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
     gene            2351..2782
                     /gene="ndk"
                     /locus_tag="OO349_002796"
     CDS             2351..2782
                     /gene="ndk"
                     /locus_tag="OO349_002796"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000963836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HCR5867798.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTC"
     gene            2932..4086
                     /gene="trmG/rlmN"
                     /locus_tag="OO349_002797"
     CDS             2932..4086
                     /gene="trmG/rlmN"
                     /locus_tag="OO349_002797"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HCR5867799.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKTFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPDAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            4371..5384
                     /gene="rodZ"
                     /locus_tag="OO349_002798"
     CDS             4371..5384
                     /gene="rodZ"
                     /locus_tag="OO349_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HCR5867800.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGVWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPVPTAATTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            5411..6538
                     /gene="ispG"
                     /locus_tag="OO349_002799"
                     /gene_synonym="gcpE"
     CDS             5411..6538
                     /gene="ispG"
                     /locus_tag="OO349_002799"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HCR5867801.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVGKIIT"
     gene            6641..7915
                     /gene="hisS"
                     /locus_tag="OO349_002800"
     CDS             6641..7915
                     /gene="hisS"
                     /locus_tag="OO349_002800"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HCR5867802.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGIFEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELLGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            7933..8553
                     /gene="yfgM"
                     /locus_tag="OO349_002801"
     CDS             7933..8553
                     /gene="yfgM"
                     /locus_tag="OO349_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HCR5867803.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGGILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            8564..9742
                     /gene="bamB"
                     /locus_tag="OO349_002802"
     CDS             8564..9742
                     /gene="bamB"
                     /locus_tag="OO349_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HCR5867804.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWAQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            9860..11332
                     /gene="der"
                     /locus_tag="OO349_002803"
     CDS             9860..11332
                     /gene="der"
                     /locus_tag="OO349_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HCR5867805.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVVNKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            11402..11617
                     /locus_tag="OO349_002804"
     CDS             11402..11617
                     /locus_tag="OO349_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HCR5867806.1"
                     /translation="MELHCPKCQHVLDQDNGHARCPSCGEFIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(11614..12984)
                     /gene="xseA"
                     /locus_tag="OO349_002805"
     CDS             complement(11614..12984)
                     /gene="xseA"
                     /locus_tag="OO349_002805"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HCR5867807.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTSVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     ARQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPKIHRTQTRIQQLEYRLAEILRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKTGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            13146..14612
                     /gene="guaB"
                     /locus_tag="OO349_002806"
     CDS             13146..14612
                     /gene="guaB"
                     /locus_tag="OO349_002806"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HCR5867808.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            14681..16258
                     /gene="guaA"
                     /locus_tag="OO349_002807"
     CDS             14681..16258
                     /gene="guaA"
                     /locus_tag="OO349_002807"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HCR5867809.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPIFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(16353..16892)
                     /locus_tag="OO349_002808"
     CDS             complement(16353..16892)
                     /locus_tag="OO349_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HCR5867810.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(16908..17426)
                     /locus_tag="OO349_002809"
     CDS             complement(16908..17426)
                     /locus_tag="OO349_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCR5867811.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQEGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            complement(17737..17928)
                     /locus_tag="OO349_002810"
     CDS             complement(17737..17928)
                     /locus_tag="OO349_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2633 family protein"
                     /protein_id="HCR5867812.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            17946..18098
                     /locus_tag="OO349_002811"
     CDS             17946..18098
                     /locus_tag="OO349_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5867813.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            18280..>19414
                     /gene="pdeF"
                     /locus_tag="OO349_002812"
     CDS             18280..>19414
                     /gene="pdeF"
                     /locus_tag="OO349_002812"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HCR5867814.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGITLGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQ"
BASE COUNT         4568 a         4729 c         5278 g         4839 t
ORIGIN      
        1 gcttatcgcc ctggcgagac gacgctgcga cgttctgttc gccatgatgc gcgacaggac
       61 tttttatacc ccgcaggggt cataacatgc ttgacaactt aataggggca cgttaccttg
      121 aagcgctgat caattatgaa gatcgctggt tctggaagca tccgggggtt aatccattct
      181 cggtggcgcg cgcagcatgg caagatctca cttcgggacg ggttatttcc ggtggcagca
      241 cgctcactat gcaggttgct cgtctgcttg atcctcaccc caaaacattt ggtggcaaaa
      301 ttctccagct ctggcgcgcg ttgcaactgg agtggcattt gtctaagcgt gaaattctga
      361 tcttgtatct taaccgcgct ccgtttggcg gtacgttgca ggggatcggt gcggcaagtt
      421 gggcttatct cggaaaatcg cctgcgaatt taagctattc cgaggcggca atgctggcgg
      481 tgttaccgca agtgcccagc cgactgcgcc cggatcgttg gccggagcgt gccgaagccg
      541 cgcgtaataa agtgctcgaa cggatggctg cgcaaggtgt gtggtcccgt gagcaggtaa
      601 aagagtcaag ggaagaaccc atctggctgg caccccgaca aatgccgcaa ctggcaccgc
      661 tgttttcgcg catgatgctc ggtaaaagca aaagcgacaa aatcgttact acgctggatg
      721 ccggtcttca acgacgtctg gaagaactgg cgcaaaactg gaaagggcgg ttgccaccgc
      781 gcagctcact ggcgatgatc gtggttaatc ataccgatat gcgtgttcgc ggctgggtgg
      841 gatcggttga tctcaacgat gattcacgct ttggtcatgt tgatatggtc aatgcgatcc
      901 gatcgccagg atcggtgctc aaaccgtttg tttatggtct ggcgctggat gaaggcttga
      961 tccacccggc atcactgctg caagacgtcc cccggcgcac cggtgattat cgaccaggta
     1021 actttgatag cggttttcat ggcccgatca acatgagcga ggcgctggtg cgctcgctga
     1081 acttacctgc tgtgcaggtg ctggaagcct atggaccgaa acggtttgcg gcaaagttac
     1141 gcaatgttgg attgccgtta tatttgccca acggcgctgc gccgaatctt tcactcattc
     1201 tcggcggcgc tggtgcaaaa ctggaagata tggcggcagc gtataccgcg tttgctcgcc
     1261 acggcaaggc aggcaaattg cgcttacagc ctgacgatcc gctgcttgaa cggcctttaa
     1321 tgtcgtcggg ggcggcgtgg atcattcgcc ggattatggc tgatgaagcg caacccttgc
     1381 cggatagtgc cttgccgcgc gtcgccccac tggcatggaa aacgggcacc agctatggct
     1441 atcgtgacgc ctgggcgatt ggggttaacg ctcgctatgt cattgggatc tggactggca
     1501 gacctgacgg cacgcccgtt gttggtcagt ttggctttgc cagtgccgta ccattgttga
     1561 atcaggtcaa taatatctta ctgtcgcgca gtgcgaatct gccggaagac ccgcgcccga
     1621 actcagtcac tcgtggcgtt atctgctggc cgggcggaca gtctttgccg gaaggtgacg
     1681 gtaactgtcg ccgccgcctg gcaacctggc tgctggatgg cagccagccg ccaacgctgt
     1741 tactgtcaga gcaggaaggg attaatggca ttcgcttccc catctggctg gatgacaaag
     1801 ggcagcgcgt cgccgctgac tgcccgcagg cgaagcaaga aatggtaaat gtctggccgc
     1861 taccgctgga accctggctg cccgcatcag aacgtcgcgc tgtgcgctta ccaccggcct
     1921 cgacaatttg cccgccatac ggtcacgatg cccagctccc tctgcagctg acaggcgttc
     1981 gcgatggcgc gattattaaa cgtttaccgg gcgcagcgga agcgactttg ccgttgcaat
     2041 caagtggagg ggcaggtgaa cgctggtggt ttctgaatgg cgaaccgtta actgaacgcg
     2101 ggcgcaacgt gaccctgcat ttgacggata aaggtgatta tcaattgctg gtgatggatg
     2161 atgtggggca aatcgcgaca gtgaaatttg tcatgcaata gtctgttttg tccctgattg
     2221 ttgctaaaac tcattttatt ttaaaaaaaa tgttaccttc atcaatagtc aacggccctg
     2281 ttgctcatta taatccgcgc catctcgtac gctggtacag acaacaacag aacaatttac
     2341 agaggtaaaa atggctattg aacgtacttt ttccatcatc aaaccgaacg cggtagcaaa
     2401 aaacgtcatt ggtaatatct ttgcgcgctt tgaagctgca gggttcaaaa ttgtcggcac
     2461 caaaatgctg catctgaccg ttgaacaggc acgtggcttt tatgctgaac acgatggaaa
     2521 accgttcttt gatggtctgg ttgaattcat gacctctggc ccgatcgtgg tttccgtgct
     2581 ggaaggtgaa aacgccgttc agcgtcaccg cgatctgctg ggcgcgacca atccggcaaa
     2641 cgcactggct ggtactctgc gcgctgatta cgctgacagc ctgaccgaaa acggtaccca
     2701 cggttctgat tccgtcgaat ctgccgctcg cgaaatcgct tatttcttcg gcgaaggcga
     2761 agtgtgcccg cgcacctgtt aataatttcg taaatgccgc gtgcaaacgt ggcatccgtg
     2821 cgccagaatt tgtacaatgc agcgcccccg gacgagcagc cgctcaccgg ggcgtttctt
     2881 ttttcaaccc tccaggggcc ataacgtgta ataacgaggc cggaatagca tatgtctgaa
     2941 caattagtca cacctgaaaa cgtcaccacg aaagatggaa aaatcaacct gctggatctc
     3001 aaccgtcagc agatgcggga gttttttaaa gatttaggtg aaaaaacctt ccgcgccgat
     3061 caggtgatga agtggatgta tcactattgc tgcgacaact ttgatgagat gaccgacatc
     3121 aacaaagtgt tgcgcggcaa actgaaagag gtggcggaaa tccgcgcacc ggaagtggtt
     3181 gaagaacagc gttcatccga cggcaccatt aaatgggcga tcgccgttgg cgatcagcgc
     3241 gtcgaaacgg tgtatatccc ggaagacgac cgtgccacgc tctgcgtctc ttcgcaggtg
     3301 gggtgtgcgc tggagtgtaa attctgttcc accgcccagc agggctttaa ccgcaacctg
     3361 cgggtatcgg aaattattgg ccaggtgtgg cgtgcggcga aaatcgtcgg cgcagcaaaa
     3421 gtcaccggtc agcgcccaat cactaacgtc gtgatgatgg gcatgggcga gccgctgctc
     3481 aacctgaaca acgtcgttcc ggcgatggaa atcatgcttg atgatttcgg ttttggcctg
     3541 tctaaacgtc gcgtgacgct ttccacttcc ggtgtagttc cggcgctgga taaactgggc
     3601 gatatgatcg acgttgcact ggcaatttcc ctgcacgcgc cgaacgacga aattcgtgac
     3661 gaaattgtgc cgatcaacaa aaagtacaat atcgaaacgt tccttgctgc ggtgcgccgt
     3721 tatctggaga aatccaacgc caatcagggc cgagtcacta ttgaatacgt gatgcttgac
     3781 cacgttaacg acggcactga acacgcgcac caactggcgg aactgctgaa agatacgccg
     3841 tgtaagatca acctgatccc gtggaacccg ttcccggacg cgccgtatgg acgcagttcg
     3901 aacagccgta tcgaccgttt ctcaaaagtg ctgatgagct acggtttcac cactattgtg
     3961 cgtaaaacgc gtggtgatga tatcgatgct gcctgtggtc agttggcggg cgatgttatc
     4021 gaccgtacga aacgtactct gcgtaaacgg atgcagggtg aagccatcga tattaaagcg
     4081 gtctgatagc cattacgcca cggttacttt atgtaccgtg gcgtaatctt ttatgaatga
     4141 agtattaagt tgcaattgtc tggtcaatat taacggtgcg tttttgctga ctttaaggca
     4201 gtatgtaacg atgcaacagt aagttagcct cagtgaatgt gggctttgtc acgagcacac
     4261 agacggtctt atactgtatg ataacggtta acttaacgga tgtttcgcgg tgtgggtgag
     4321 catgatggtt caccggcatc tcaattctca tttaaacgta cctgcagcga atgaatactg
     4381 aagccacgca cgaccaaaat gaagcactta ctaccggcgc tcgcctgcgt aatgctcgcg
     4441 aacaactagg acttagtcag caggccgttg ccgagcgact ttgcctgaag gtttccacgg
     4501 tacgcgacat tgaagaagat aaggcacccg ccgatcttgc ttcaacattc ctgcgcggat
     4561 atatccgctc ttatgcgcgt ctggtacata ttccagaaga agaactgctg ccagggctgg
     4621 aaaagcaggc tccacttcgg gctgcaaaag ttgcgccaat gcagagtttt tccctcggta
     4681 aacgccgcaa aaaacgcgac ggctggctaa tgaccttcac ctggctggtg ttgtttgtgg
     4741 ttatcggcct gagcggtgtc tggtggtggc aagaccacaa agctcagcag gaagagatca
     4801 ccactatggc cgatcaatct tcggcggaac tgagcagtaa tagcgagcag gggcagagtg
     4861 ttccgttaaa tacgtcgaca actacagacc cggctacaac gtccacgccg ccagcgtctg
     4921 tggatactac cgcaaccaac acgcaaacac ctgccgtaac tgcgccagca ccagctgttg
     4981 atccgcaaca gaatgcggtt gtttcgcctt cgcaggcaaa tgttgatacc gccgcgaccc
     5041 cggtaccaac ggcagcaaca acgccagatg gtgctgcgcc gttgccaacc gatcaggctg
     5101 gcgtgaccac gccggcggct gatccgaatg cgctggtgat gaactttact gccgattgct
     5161 ggctggaggt cactgatgct accggtaaaa aattgtttag cgggatgcag cgtaaagacg
     5221 gtaatttgaa tttaaccggc caggcaccgt ataaactgaa aattggtgcg ccagccgcag
     5281 tacagatcca gtatcaaggg aaacctgtcg atctgagtcg ttttatcaga actaaccagg
     5341 ttgcgcgtct gaccctcaat gccgaacaat caccggcgca gtaacagacg ggtaacgcgg
     5401 gagatttttc atgcataacc aggctccaat tcaacgtaga aaatcaacac gtatttacgt
     5461 tgggaatgtg ccgattggcg atggtgctcc catcgccgta cagtccatga ccaatacgcg
     5521 tacgacagat gtcgaagcaa cggtcaatca aatcaaggcg ctggaacgcg ttggcgctga
     5581 tatcgtccgt gtctccgtac cgacgatgga cgcggcagaa gcgttcaaac tcatcaaaca
     5641 gcgggttaac gtgccgctgg tggctgacat ccacttcgac tatcgcattg cgctgaaagt
     5701 agcggaatac ggcgtcgatt gtctgcgtat taaccctggt aatatcggta atgaagagcg
     5761 tattcgcatg gtggttgact gtgcgcgcga taaaaacatt ccgatccgta ttggcgttaa
     5821 cgccggatcg ctggaaaaag atctgcaaga aaagtatggc gaaccgacgc cgcaggcgtt
     5881 gctggaatcc gccatgcgtc atgttgatca tctcgatcgc ctgaacttcg atcagttcaa
     5941 agtcagcgtg aaagcgtctg acgtcttcct cgctgttgag tcttatcgtt tgctggcaaa
     6001 acagatcgat cagccgctgc atctggggat caccgaagcc ggtggtgcac gcagcggggc
     6061 agtaaaatcc gccattggtt taggtctgct gctgtcggaa ggcatcggcg acacgctgcg
     6121 tgtatcgctg gcggccgatc cggtcgaaga gatcaaagtc ggtttcgata ttttgaaatc
     6181 gctgcgtatc cgttcgcgag ggattaactt catcgcctgc ccgacctgtt cgcgtcagga
     6241 atttgatgtt atcggtacgg ttaacgcgct ggagcaacgc ctggaagata tcatcactcc
     6301 gatggacgtt tcgattatcg gctgcgtggt gaatggccca ggtgaggcgc tggtttctac
     6361 actcggcgtc accggcggca acaagaaaag cggcctctat gaagatggcg tgcgcaaaga
     6421 ccgtctggac aacaacgata tgatcgacca gctggaagca cgcattcgtg cgaaagccag
     6481 tcagctggac gaagcgcgtc gaattgacgt tcagcaggtt ggaaaaataa taacgtgatg
     6541 ggaagcgcct cgcttcccgt gtatgattga acccgcatgg ctcccgaaac attgagggaa
     6601 gcgttgaggg ttcattttta tattcagaaa gagaataaac gtggcaaaaa acattcaagc
     6661 cattcgcggc atgaacgatt acctgcctgg cgaaacggcc atctggcagc gcattgaagg
     6721 cacactgaaa aacgtgctcg gcagctacgg ttacagtgaa atccgcttgc cgattgtaga
     6781 gcagaccccg ctattcaaac gtgcgattgg tgaagtcacc gacgtggttg aaaaagagat
     6841 gtacaccttt gaggatcgca atggcgacag cctgactctg cgccctgaag ggacggcggg
     6901 ctgtgtacgc gccggcatcg agcatggtct tctgtacaat caggaacagc gtctgtggta
     6961 tatcgggccg atgttccgtc acgagcgtcc gcagaaaggg cgttatcgtc agttccatca
     7021 gttgggctgc gaagttttcg gtctgcaagg tccggatatc gacgctgaac tgattatgct
     7081 cactgcccgc tggtggcgtg cgctgggtat cttcgaacac gtaactcttg agctgaattc
     7141 tatcggttcg ctggaagcac gcgccaatta ccgcgatgcg ctggtggcat tccttgagca
     7201 gcataaagaa aagctggacg aagactgcaa acgccgcatg tacactaacc cactgcgcgt
     7261 gctggattca aaaaatccgg aagtgcaggc gcttctcaac gacgctccgg cattaggtga
     7321 ttatctggac gaggaatctc gtgagcattt tgccggtctg tgcaaactgc tggagagcgc
     7381 ggggatcgct tacaccgtaa accagcgtct ggtgcgtggt ctggattact ataaccgtac
     7441 cgttttcgag tgggtgacta acagtctcgg ctcccagggc accgtgtgtg caggcggtcg
     7501 ttatgacggt cttgtggaac aactgggcgg tcgtgcaaca ccggctgtcg gttttgcgat
     7561 gggcctcgaa cgtcttgtat tgttagtaca ggccgttaat ccggaattta aagccgatcc
     7621 tgttgtcgat atatacctgg tggcttcagg tgctgataca caatctgcgg ctatggcatt
     7681 agctgagcgt ctgcgtgatg aattactggg cgtgaaattg atgaccaacc acggcggcgg
     7741 caactttaag aaacagtttg cccgtgctga taaatggggt gcccgcgttg ctgtggtgct
     7801 gggtgagtct gaagtggcta acggcacagc agtagtgaag gatttgcgct ctggtgagca
     7861 aacggcagtt gcgcaggata gcgtagccgc gcatttgcgc acgttactgg gttaaggaag
     7921 gagaaggaca gcgtggaaat ttacgagaac gaaaacgacc aggtagaagc ggttaaacgc
     7981 ttttttgctg aaaatggcaa agcactggct gttgggggga ttttgggcgt tggcgcactg
     8041 attggctggc gctactggaa cagccatcag gttgattctg cacgctccgc ttctcttgcc
     8101 tatcaaaatg cggttaccgc agtgagcgaa ggcaaaccgg atagcatccc ggcggcggaa
     8161 aaatttgctg ctgaaaataa aaatacttat ggtgcgctgg cttctttgga acttgcgcag
     8221 caatttgttg acaaaaatga actagagaaa gctgccgccc agttacaaca ggggctggca
     8281 gacacgagcg atgaaaatct caaagccgtg ataaatctgc gtcttgctcg cgttcaggta
     8341 cagctcaagc aggctgatgc cgcgctgaaa acccttgata ccatcaaagg tgaagggtgg
     8401 gctgccattg ttgccgactt gcgtggtgaa gcattgctga gcaaaggtga taagcaaggt
     8461 gcgcgtagtg catgggaagc aggcgtgaaa agcgatgtta ctccggcact gagcgaaatg
     8521 atgcagatga aaattaataa tttgtccatc tgagagggac ccgatgcaat tgcgtaaatt
     8581 actgctgcca ggactgcttt ccgttaccct tttaagcggc tgttcgctgt ttaacagcga
     8641 agaagatgtg gtaaagatgt ccccattgcc aaccgttgaa aaccagttta cgccgaccac
     8701 ggcgtggagc acttccgttg gtagcggcat tggcaacttc tattccaatc ttcatccggc
     8761 actggcggac aacgttgtct atgcagcgga ccgcgctggt ttggtaaaag cgctgaatgc
     8821 ggatgatggc aaagaaatct ggtctgtcag cctggccgag aaagatggct ggttctctaa
     8881 agagcctgca ttactttctg gcggtgtgac cgtgtctggt gggcatgtct acattggcag
     8941 cgaaaaggcg caggtttacg cgctgaatac cagcgatggt actgtggcat ggcaaactaa
     9001 agtcgcgggt gaagcacttt cgcgcccggt ggtcagcgac ggtctggtgt taatccacac
     9061 cagtaacggt cagttacaag cgctgaacga agctgacggc gctgtcaaat ggacagttaa
     9121 cctcgatatg ccttcgctct ctttgcgtgg cgagtctgcg ccggcaacgg cttttggtgc
     9181 ggccgtcgtg ggtggcgata atggtcgcgt cagcgcagtg ctgatggaac agggccagat
     9241 gatttggcag cagcgtattt cccaggcgac cggttctacc gaaattgacc gtctgagcga
     9301 tgttgacacg actcccgtcg ttgttaacgg cgttgttttc gcgctggcct ataatggtaa
     9361 cctgacggcg cttgatctgc gcagtggtca gattatgtgg aaacgcgaac tgggttcggt
     9421 gaatgatttc atcgtcgatg gcaatcgcat ctatctggtc gatcaaaatg accgggtgat
     9481 ggcgttgacc attgatggcg gcgttacgct gtgggcacaa agcgatctgc tgcatcgcct
     9541 gctgacttct ccggtgctgt ataatggcaa cctggtggtc ggtgacagtg aaggttatct
     9601 gcactggatt aacgtcgaag atggtcgttt cgttgcccag caaaaagttg atagttccgg
     9661 tttccagact gaaccggttg ccgctgacgg caaactgctg atccaggcaa aagacggaac
     9721 cgtgtactct attacacgtt aatcgtctct gtcgttcact ttgaaaacgg ctcctggaca
     9781 ggggccgttt tcctgttttt aacaacgacg cgaatatagc gtgcgttgtc tgatgattta
     9841 taaaaatgag gctttaaaca tggtacctgt ggtcgcgctt gtcgggcgcc ctaacgtagg
     9901 aaaatccacg ttatttaacc gtctaactcg cacccgagat gcgctggttg cggatttccc
     9961 gggtctgact cgtgaccgta agtacggtcg tgcggaaatt gaaggccgtg agtttatctg
    10021 tattgatacc ggcgggattg atggcacaga agacggtgta gaaacccgca tggcggaaca
    10081 gtcgctgctg gcgattgaag aagcggacgt cgtactgttt atggtggatg cgcgcgcggg
    10141 cctgatgccg gcagatgaag cgattgccaa acatctgcgc tcccgtgaaa aaccgacctt
    10201 cctggtggta aacaaaactg acggtctgga tcccgatcag gcagtggttg atttctactc
    10261 gcttggttta ggtgaaatct acccgatcgc cgcgtctcac ggtcgtggcg tattaagtct
    10321 gctggagcat gtgctgctgc cgtggatgga agatctcgca ccgcaagagg aagtcgacga
    10381 agacgctgaa tactgggcgc aatttgaagc ggaagagaac ggcgaagaag aagaggaaga
    10441 cgacttcgac ccgcaaagtc tgccgatcaa actggcgatt gtgggtcgtc cgaacgtagg
    10501 taagtctaca ctcactaacc gtattcttgg agaagagcgc gttgttgttt acgacatgcc
    10561 tggcacgacg cgtgacagca tctacatccc aatggaacgc gatggacgtg agtatgtgct
    10621 cattgacacc gctggcgtac gtaaacgcgg caaaatcacc gatgctgtag agaaattctc
    10681 cgtaatcaaa acgttgcagg ccattgaaga cgccaacgtg gtgatgttag tgattgatgc
    10741 gcgcgaaggt atttccgatc aggatctctc gctgctgggc tttattctca atagtgggcg
    10801 ctcacttgtc attgtggtga ataagtggga tggcctgagc caggaagtga aagagcaggt
    10861 gaaagaaacg ctggacttcc gtctgggctt tatcgatttt gctcgtgtgc actttatctc
    10921 tgccttgcac ggcagtggtg ttggtaactt gtttgaatca gtacgtgaag cgtatgacag
    10981 ctccacccgt cgtgtgggga cctctatgct gacgcgcatc atgacgatgg ctgttgaaga
    11041 tcaccaaccg ccgctggtac gcggtcgtcg tgtgaagctg aaatatgccc acgctggtgg
    11101 ttataacccg ccgattgtgg tgattcacgg taatcaggtg aaagacctgc ctgattccta
    11161 caagcgctac ttgatgaact acttccgcaa atcgctggac gtaatgggat cgccgattcg
    11221 tattcagttc aaagaagggg aaaacccgta tgcgaacaag cgtaacaccc tgacgccaac
    11281 ccagatgcgt aaacgtaagc gtctgatgaa gcacatcaag aaaaataaat aattatccgt
    11341 aagtatttta tctgccattc cctctatgaa tgtagaggga atggctaccg gaggaattca
    11401 tatggaactg cattgtccaa aatgtcagca tgtgcttgat caggataacg gccatgcgcg
    11461 ttgcccatcc tgcggggaat ttattgaaat gaaagctctt tgtccagact gccatcaacc
    11521 gttgcaggtg ttaaaagcct gcggtgcagt agattacttc tgtcagcacg ggcatggctt
    11581 gatatcgaaa aaacgcgttg aattcgtgct ggcttaatga acctttttac gcgatttttt
    11641 taccggctgg atgtttttta cttcactttc tatccagccg tcttccagac gtgtggttag
    11701 catttcaccc gttttaactt gcttcacttt tttcagtaca ttgccgtcag tagcagtagt
    11761 aacgctgtat ccacgcgcca gcgttgacag tgggcttacg gcttcgaggt gcgttactgc
    11821 attaccgaaa cgttcacgcg tggcgctaag ctgtgcgcgc aggatttctg ctaaacgata
    11881 ttccagttgc tgaatgcgcg tttgcgtgcg atgaatcttc ggctgtggat tttgctgatt
    11941 cagccgctgt gttaatcgct gctgctgttg cccggcacgc ttaagctgat tttccagcgc
    12001 aaagctcatc cgtttttgca ggcgttcaag catggtttgc tggcgtgcca gccggagctg
    12061 cggatgctgc tgctgcaagc gatgatgaat ctgcgtaaag cgacgtgtac ggttggcgag
    12121 ataataatcc atcgccatct ccagccgttg acgggccgat tgcacctggc gcagtaactc
    12181 ttgctgatta cggctcacta cttcagcggc ggcagacggc gttggcgcac gcagatcggc
    12241 aacaaaatcg gcaatggtca catccgtctc atgcccgacg gcgctgacga ccggaatgcg
    12301 gctggcaaaa atcgcccgcg ctacgcgttc gtcgttaaaa ctccataaat cttccagcga
    12361 accgccgccg cgcccaacga tcaacacgtc gcactcattg cgctgattcg ccagttcaat
    12421 ggcgcgaaca atttgccccg gcgcgtcatc gccctgaacg gaggtggggt agatgatcac
    12481 cggtagagaa ggatcgcgac gttttaacac atgcaaaata tcatgtagcg cagcaccggt
    12541 ttttgaggtg atcacaccaa cgcaatgcgc aggggaggga agtggttttt tgtattgctg
    12601 atcgaacaaa ccttcagcct gcaacttcgc tttgagctgt tcgtacttct gttgcagcag
    12661 cccttcaccg gccggctgca tactctcaac gattatctgg tagtcgccgc gcggctcgta
    12721 gagcgtaata ttggcgcgaa ctaaaacttg ttgcccatgc tgcggacgga aggtgacccg
    12781 gcggttgctg ttgcggaaca tcgcgcagcg tacctgggcg gtgtcgtctt tgagtgtaaa
    12841 gtaccagtga ccggaagccg gttgtgtgaa attagagatt tcgccgctga tccaaacctg
    12901 tcccatctca tgctcaagca gcagacgaac cgtttgattc aggcgactaa cggtaaaaat
    12961 tgcaggggat tgagaaggta acatgtgagc gagatcaaat tctaaatcag caggttattc
    13021 agtcgatagt aacccgccct tcggggatag caagcatttt ttgcaaaaag gggtagatgc
    13081 aatcggttac gctctgtata atgccgcggc aatatttatt aaccactctg gtcgagatat
    13141 tgcccatgct acgtatcgct aaagaagctc tgacgtttga cgacgttctc ctcgttcctg
    13201 ctcattctac cgttctgccg aatactgctg acctcagcac ccagctgacg aaaactattc
    13261 gtctgaatat ccctatgctt tccgcagcaa tggataccgt aacggaagcg cgcctggcta
    13321 ttgctctggc tcaggaaggc ggtatcggct ttatccacaa aaacatgtcc attgaacgcc
    13381 aggcagaaga agttcgccgt gtgaaaaaac acgaatctgg tgtggtgact gatccgcaga
    13441 ctgttctgcc aaccacgacg ctgcgcgaag tgaaagaact gaccgagcgt aacggttttg
    13501 cgggctatcc ggtcgttacc gaagaaaacg aactggtggg tattatcacc ggtcgtgacg
    13561 tgcgttttgt taccgacctg aaccagccgg ttagcgttta catgacgccg aaagagcgtc
    13621 tggtcaccgt gcgtgaaggt gaagcccgtg aagtggtgct ggcaaaaatg cacgaaaaac
    13681 gcgttgaaaa agcgctggtg gttgatgacg aattccacct gatcggcatg atcaccgtga
    13741 aagacttcca gaaagcggaa cgtaaaccga acgcctgtaa agacgagcaa ggccgtctgc
    13801 gtgttggtgc tgcagttggc gcaggtgcgg gtaacgaaga gcgtgttgac gcgctggttg
    13861 ccgctggcgt tgacgttctg ctgatcgact cctcccacgg tcactcagaa ggcgttttgc
    13921 agcgtatccg tgaaacccgt gctaaatatc cggatctgca aatcatcggc ggcaacgtgg
    13981 caacagctgc aggtgcacgc gctttggcag aagctggttg cagcgcggtt aaagtcggta
    14041 tcggccctgg ttctatctgt acgactcgta tcgttactgg cgtaggtgtt ccgcagatca
    14101 ctgccgttgc tgacgcagtt gaagccctgg aaggcaccgg tattccggtt atcgcagatg
    14161 gtggtattcg cttctccggc gacatcgcca aagctatcgc cgctggcgca agcgcggtga
    14221 tggtaggttc catgctggcg ggtactgaag aatctccggg tgaaatcgaa ctctaccagg
    14281 gccgttctta taaatcttac cgtggtatgg gttccctggg cgcgatgtcc aaaggttcct
    14341 ctgaccgtta tttccagagc gataacgctg ccgacaaact ggtgccggaa ggtatcgaag
    14401 gtcgcgtagc ttataaaggt cgcctgaaag agatcattca ccagcagatg ggcggcctgc
    14461 gctcctgtat gggtctgacc ggctgtggta ctatcgacga actgcgtact aaagcggagt
    14521 ttgtacgtat cagcggtgcg ggcattcagg aaagccacgt tcacgacgtg accattacta
    14581 aagagtcccc gaactaccgt ctgggctcct gattctcttc gcccgatttc atgtcgggcg
    14641 atttatatta tctgtttcac ttgcctcgga ataagcgtca atgacggaaa acattcataa
    14701 gcatcgcatc ctcattctgg acttcggttc tcagtacact caactggttg cgcgccgcgt
    14761 gcgtgagctg ggtgtttact gcgaactgtg ggcgtgggat gtgacagaag cacaaattcg
    14821 tgacttcaat ccaagcggaa ttattctttc cggcggcccg gaaagtacta ctgaagaaaa
    14881 cagtccgcgt gcgccgcagt atgtctttga agcaggcgta ccgatattcg gcgtttgcta
    14941 tggcatgcag accatggcaa tgcagttggg cggtcacgtt gaagcctcta acgaacgtga
    15001 atttggctac gcgcaggtag aagtcgtaaa cgacagcgca ctggttcgcg gtatcgaaga
    15061 tgcgctgacc gcagacggta aaccgctgct cgatgtctgg atgagccacg gcgataaagt
    15121 taccgctatc ccgtccgact tcgtcaccgt agccagcacc gaaagctgcc cgtttgccat
    15181 tatggctaac gaagaaaaac gcttctatgg cgtacagttc cacccggaag tgactcacac
    15241 ccgccagggt atgcgcatgc tggagcgttt tgtgcgtgat atctgccagt gtgaagccct
    15301 gtggacgcca gcgaaaatta tcgacgatgc tgtagctcgc atccgcgagc aggtaggcga
    15361 cgataaagtc atcctcggcc tctctggtgg tgtggattcc tccgtaaccg caatgctgct
    15421 gcaccgtgct atcggtaaaa acctgacttg cgtattcgtc gacaacggcc tgctgcgtct
    15481 caacgaagca gagcaggttc tggatatgtt tggcgatcac tttggtctta acattgttca
    15541 cgtaccggca gaagatcgct tcctgtcagc gctggctggc gaaaacgatc cggaagcaaa
    15601 acgtaaaatc atcgggcgcg ttttcgttga agtattcgat gaagaagcgc tgaaactgga
    15661 agacgtgaag tggctggcgc agggcaccat ctaccctgac gttatcgaat ctgcggcttc
    15721 tgcaaccggt aaagcacacg tcatcaaatc tcaccacaac gtgggcggcc tgccgaaaga
    15781 gatgaagatg ggcctggttg aaccgctgaa agagctgttc aaagacgaag tgcgtaagat
    15841 tggtctggag ctgggcctgc cgtacgacat gctgtaccgt catccgttcc cgggaccagg
    15901 ccttggcgtt cgtgttctgg gtgaagtgaa gaaagagtac tgtgacctgc tgcgccgtgc
    15961 tgacgccatc ttcattgaag aactgcgtaa agcggacctg tacgacaaag tcagccaggc
    16021 gttcaccgtc ttcctgccag tacgttccgt tggcgtaatg ggcgatggtc gtaagtatga
    16081 ctgggttgtc tctctgcgtg ctgtcgaaac catcgacttt atgaccgcac actgggcgca
    16141 tctgccgtac gatttcctcg gtcgcgtttc caaccgcatt atcaatgaag tgaacggtat
    16201 ttcccgcgtg gtgtatgaca tcagcggcaa gccgccagct accattgagt gggaatgatt
    16261 ttgatccctg cactatgaat gaacaaaacc ctccgttact acagagggtt ttttatcttc
    16321 aagaatcata ggcttgaagt tactaacatc gattaattaa accagctgtc cgatttgttc
    16381 tcttctgctt tgcccacgct tttcatcaga tcgcgaccgc cttcagtcat atttctgttt
    16441 gcgtcagctt cagattgcac cacatcggtt tgcgcagctt tgtgcttcag ttcctgatcg
    16501 ataaattcgt tttcgcgctt aacgcgggct tcttctttcg ccagcgccag tttttgtttc
    16561 tgaatctcta agctgcgtag ctcatcttca taactttgat cgcgtttttt gtccgcagtg
    16621 gcttcggcgt ccagtttatc ctgacgagct ttcttatttg ccgctgccgt tgccgctctt
    16681 ttgttcgcgg cggcctgggc atttgcgcga cgttgcttct cttgctggat ttccctgttg
    16741 cgctccgcga cccattcgtc atgctgcctt tgctcttcat ttttaccttg ctgttccgct
    16801 tctgcgacag cagagagttg atcctgcaat gatgaggcta tagccggata gcttaaggag
    16861 gctaagatgg cgcaaagaaa aactttcttc atgactcctc ctgattatta gctcttttca
    16921 ggacatttgg tatttggctg aatacgcgtt tcgttatacg ttgtggtaat aacaacggct
    16981 aaacctgtcg taaactggca ctctttaccc tcctgggtgg aggtatacac tttggtgcct
    17041 tccttatatg ttaaagaaac accttccact aaggttttat cattcaccat agaacccgct
    17101 gccgcgccta cagctccgcc gccaactgcc cctgccgtcg ttccggaatt gctgccagaa
    17161 ccgacgttgt ggccgataac accgccagcg actgcgccaa taagcgcgcc gaaggcttgt
    17221 gcgttccgtt tattttggga gttgtctacg gcaacttttg cgggaagaat ggaaataata
    17281 ttaacggttt tagtttcttg tttggtattc agttgatcgg tttgataaac atcggcggca
    17341 tgatcgtcag catttgactg gcatcctgcc agagtgaatg acgctaacat tgccacaggc
    17401 agaagacatt ttttaaattt catcactatt ccttgttata cacattacga aaaataagac
    17461 atttaagatc atcaaaagag gaaagtaaat gtattgcgta taaagggata gattaaattt
    17521 attaaaattc acgcagggaa taatttataa aaatatatca ttacaacata tatattaatt
    17581 ttatatttat ttcgcagcca aacaggataa acatcagtca ggacaaagac gtaaaattta
    17641 agatgaaaga atatctgccg ctttgataat cccaaagagc aaaagcatcc gacagcgaag
    17701 tgccgttatg ttctgtgaaa gtggagaagg taaccgctaa cgttgtaccg gtgattcgac
    17761 ggagagtgtg gattgctgag cttctttttt gctctgatgg tgctgccagg caccgacgga
    17821 cgagtagata aaacggccaa agaagaagat aaagctgatg agcagtacga tacgggtcat
    17881 gcgactgtta aatcggtgtc gttttcgcat actagttgcc tgactcacaa aaggttcctt
    17941 gaagtatgtc ccacgctgtg gacggtactt acattaaggc acaacaggac aaaatggtca
    18001 attcttcgtt atgtaaaaaa gcgtcagtgg atacatattt taatgttatg gaagttaatt
    18061 taattattta caatgatgat gtaataatga tgaattaaca tgataaacgt aataattcat
    18121 taatcagggt tattttgttt gatatatatc aattggaact ttcatatgac acttagaatc
    18181 aatgctctct atagtgatga ataatcatca ttcgaagtca ggtgggatgc ctgtctgaat
    18241 acacctcctt caggatgtgg gggattctgc tgagcatcta tgaaactgaa tgcaacttat
    18301 ataaaaatac gtgataaatg gtgggggctt ccgctgttcc tgccttcttt aatcttgccc
    18361 attttcgccc acattaatac tttcgcgcat atttcttccg gtgaggtttt tctcttttat
    18421 ctgcctttgg cactgatgat cagcatgatg atgtttttca gctgggcggc attgccaggg
    18481 atcaccttag ggatttttgt ccgcaaatat gcagagctgg gtttttacga aacgctctca
    18541 ttaacggcta attttattat cattatcatt ctctgttggg gcggttacag ggtctttact
    18601 ccccggcgta acaacgtttc acatggtgat acccgtttaa tttcccagcg tatattctgg
    18661 cagatagtgt ttcctgcaac gctgtttctg atacttttcc agtttgctgc atttgtagga
    18721 ttactggcga gcagagaaaa tctggtcggt gtcatgcctt ttaacctcgg gactttaatc
    18781 aattatcagg ccttactggt gggtaatctg atcggtgtcc cgctgtgcta cttcatcatt
    18841 cgggtagtgc gaaatccatt ttatttacgt agctattatt cgcaattaaa acagcaggtt
    18901 gatgccaaag tcaccaaaaa agagttcgcg ctctggctac tggcattagg tgctttattg
    18961 ttgctgttat gcatgccgtt aaatgaaaaa agcacaattt ttagcaccaa ttataccttg
    19021 tcattattgc tgcccctgat gatgtgggga gcaatgcgct atggttataa gctgatttcg
    19081 ctgctctggg cggtcgtgtt gatgatcagc atccacagct atcaaaatta cattcccatt
    19141 tatcctggct ataccacgca gctgaccata acctcctcca gttatctggt attctctttt
    19201 attgtcaatt atatggctgt actggcaact cgtcagcgag cggtagtcag acgcattcag
    19261 cggcttgcgt atgtggaccc ggtggttcat ctgccaaatg ttcgcgccct gaatcgcgcg
    19321 ttgcgtgatg ccccctggtc tgcgctttgt tatttacgca tccctggcat ggaaatgctg
    19381 gttaagaact atggcatcat gctgcggatt caat
//
LOCUS       DAJTQY010000077        19260 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.077, whole genome shotgun sequence.
ACCESSION   DAJTQY010000077 DAJTQY010000000
VERSION     DAJTQY010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19260
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.077"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            31..330
                     /locus_tag="OO349_002813"
     CDS             31..330
                     /locus_tag="OO349_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HCR5867815.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGMVTIKGVAERRITY
                     RREGYEYDCVMPVYQFRRDFTLVQAAPRSKPTSREKARANIQEIKKMLNVFRGKK"
     gene            327..2075
                     /locus_tag="OO349_002814"
     CDS             327..2075
                     /locus_tag="OO349_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCR5867816.1"
                     /translation="MKLAPNVKKQPRGIKHKDTEVIIFAGSDAWSHAKQWQEQDGPAS
                     GDNVPPVWLGPNQLAELDALKIVPDGKKRVRLYQAGELDLVETKKIGQKLAAADIQDA
                     NFYPEGMHVQKCENWRRYLNAERENIAAGLTMPEQKNTQLAQMADSERAQLLAERFDG
                     VCVHQESEIVHVWRGGVWCPVSTMELSREMVAIYSEHRATFSKRVINNAVEALKVIAE
                     PMGEPSGDLLPFANGALDLKTGEFSPHTPENWITTHNGIEYTPPAPGENIRDNALNFH
                     KWLEHAAGKDQRKMMRICAALYMIMANRYDWQMFIEATGDGGSGKSTFTHIASLLAGK
                     QNTVSAEMTSLDDAGGRAQVVGSRLIVLADQPKYTGEGTGIKKITGGDPAEINPKYEK
                     RFTAVIRAVVLATNNNPMIFTERAGGVARRRVIFRFDNIVSEAEKDRELPEKIAAEIP
                     VIIRRLLANFTDSEKARALLLEQRDGDEALAIKQQTDPVIEFCQFLNFLEEARGLMMG
                     GGGDSVKYTTRNSLYRVYLAFMAYAGRSKPLNVNDFGKAMKPAAKVYGHEYITRKVKG
                     VTQTNAITTDECDAFL"
     gene            2364..2642
                     /locus_tag="OO349_002815"
     CDS             2364..2642
                     /locus_tag="OO349_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707047.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5867817.1"
                     /translation="MHTSGKFNKSFKKRRDRTEPKYRALDMTEHALKVAIRTIDRHAG
                     EGYAKAHPELISAFMTTTAANFATLTEREIAEAEQVTTINVKTGEVES"
     gene            2639..2944
                     /locus_tag="OO349_002816"
     CDS             2639..2944
                     /locus_tag="OO349_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5867818.1"
                     /translation="MSEPKFGEKFYKHNGRITILQISAATPGWWVETDEGSSPVASWA
                     LCVLSYPDRDVYQDILPVISTDKGMKPVDIKNMGFQCVMLTEKMMEEMKKNNAGSLH"
     gene            2954..3115
                     /locus_tag="OO349_002817"
     CDS             2954..3115
                     /locus_tag="OO349_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HCR5867819.1"
                     /translation="MTAQIAAYGRLVGDPLVKQSSKGTPEPPTENYADLRNAVNRTYA
                     DMGLSKLAE"
     gene            3257..3481
                     /locus_tag="OO349_002818"
     CDS             3257..3481
                     /locus_tag="OO349_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309616.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5867820.1"
                     /translation="MPMKFDEILKQRDKYHADNMETMNITDYRAFLETGALIEKDHHG
                     FVRCALSGEMLAVNPEQTDALIEFLKGIRD"
     gene            3782..4939
                     /locus_tag="OO349_002819"
                     /pseudo
     CDS             3782..4939
                     /locus_tag="OO349_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309617.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
     gene            4979..5551
                     /locus_tag="OO349_002820"
     CDS             4979..5551
                     /locus_tag="OO349_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HCR5867821.1"
                     /translation="MGEKIMKSMEIRSSEITTSGAGTLTGYVVRWDKLSELLWGEFYE
                     KFQRGAFTEWLAAGNDVRGLYEHDHSMLLGRTRSGTLKLEEDETGLRFELTPPDTSTG
                     RDVIELVKRGDISGMSFGFRSRKDVWDTTTDPCVRTVLVAELYEITVTSVPAYPDSGV
                     ELARRSLYEQHPEKMPRADNRRWWADLAGV"
     gene            5553..6764
                     /locus_tag="OO349_002821"
     CDS             5553..6764
                     /locus_tag="OO349_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCR5867822.1"
                     /translation="MWPFRRKKEQRSMTLDEFMALAGTSNTGAGEYVSSGTAESLPAV
                     MNAVTVISDAVATMPCYLYLVRNEKGKEAREWLDSHPVDHILNERPNAWQTPYQFKRM
                     MIRYCLLSGNAYAVIQWGRDGFPAALHPYPPQSVNVEQTGDHNWRYCITDAYTGNTRN
                     YLPWEVLHLRYSTDDGFMGRSPVTICRESLGLGLAQQRHGASVMRDGMMAAGVITSGE
                     WLDGVKGKQALAALERYKGARNAGKTPILEGGMSYQQLGMSNQDAEWLASRRFTIEDI
                     ARMFNVSPIFLQEYSNSTYSNFSEASRAFLTMTMRPWLANFEQQIKNALLVASPVPGI
                     RYQVEFDSADLLRATPGERFATYERGIKSDVMCPNEAREREGLSPRDGGDEFSQAWKQ
                     EVKISEGEKPE"
     gene            6761..7099
                     /locus_tag="OO349_002822"
     CDS             6761..7099
                     /locus_tag="OO349_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HCR5867823.1"
                     /translation="MNIGRLRDRITIQTLKQTRDITGEILETWEDGHTLWASVNMVSS
                     KEAISSGAELAIGTVRIWIRYRKDINATSRIKVSTGPLAGRVLNIIGQPLPDAARTRL
                     EILCREGAEK"
     gene            7096..7392
                     /locus_tag="OO349_002823"
     CDS             7096..7392
                     /locus_tag="OO349_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HCR5867824.1"
                     /translation="MTEELITLEEVKLHCRIDGDDEDQLISGYIAASLEACQIHIGRR
                     FDDGLAFTPAIKIGCMMFIAHLYENRQIVADNAKTRVPMTIGALWTAYRDVGGY"
     gene            7392..7832
                     /locus_tag="OO349_002824"
     CDS             7392..7832
                     /locus_tag="OO349_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707053.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR5867825.1"
                     /translation="MPWQPLRRCTEPGCNRRVKSGKCEEHRRAAWRAEDARRGHRRAR
                     GYSRQWDKYRAMYLSKNPLCVRCLEKGIYTPAVVVDHIIPINGGDDVLFWPEWNHQPL
                     CQACHNQKTKWLDPATKSKRAAGGFREEEERAANRNNWMYDADE"
     gene            7816..7998
                     /locus_tag="OO349_002825"
     CDS             7816..7998
                     /locus_tag="OO349_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5867826.1"
                     /translation="MTLMNEREQNRLIRGLIRQRDAWKTQETGHKDKASGRAERITAK
                     RLTDRDREVMECFRNR"
     gene            8054..8344
                     /locus_tag="OO349_002826"
     CDS             8054..8344
                     /locus_tag="OO349_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HCR5867827.1"
                     /translation="MDDIAVKQWREKSRQLAEQGDLTPADWSNLELYCVNYSIYRKAV
                     ADLAARGFSIVNSQGGESRNPALSAKSDAERVMIKMASLLGFDPISRRKNPP"
     gene            8364..10025
                     /locus_tag="OO349_002827"
     CDS             8364..10025
                     /locus_tag="OO349_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCR5867828.1"
                     /translation="MTAWNKYAEDVKTGKIPACKRLKQAVKRYFSDLKSPLYTFDREV
                     VERFIAFSRVCPHVKGPMRGRPIELEPWQQFAFACILGFKVKATGRRKYTSAFIEVPR
                     KNAKSTTAAILANWFLIMENGQQDIYTAAVSRDQARIVFDNARQMCLLSRPLRRRVNI
                     QAHKVIHPKSNSLLKPLAAKAATIEGTNPSLSIVDEYHLHPDNGVYSALELGMGARPE
                     GLLFAITTSGSNVVSACKQHYDYCCQILDGEEVNDSIFVLIYELDDENEVDDPAMWIK
                     ANPNIDVSVDREKLASTIQKARGIPSQWVEMMTKRFNIWCQGTTPWMGNGAWAECAGT
                     FTEEDLHGQECYAGLDLSSTSDISSVCYAFPVGKTIMLISRHYLPEFQLQNPANKNRA
                     VYRQWAKAGWIRTTPGDCIDYDRIRDDIMQDAEKFNIRLVGFDTWNATHLRTQLQGAG
                     FEVEPFPQTYLRFSPAAKSFEVFVNRRVIVHRGDPVLSWSMSNVVMQSDANANIKPNK
                     KKSPNKIDPSVAALMAFGTFQAEHEDFAFDISDSHRQKLEEFSGV"
     gene            10039..10320
                     /locus_tag="OO349_002828"
     CDS             10039..10320
                     /locus_tag="OO349_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5867829.1"
                     /translation="MTEAEILRLIRRVSGISQQADEQTTQPDSVTAENYARVVAEVMR
                     RDGIQLNDADMRDIRIRVLEMLAYNRRVELYREKEKITYHWKKPERLRR"
     gene            complement(11117..12577)
                     /gene="phoQ"
                     /locus_tag="OO349_002829"
     CDS             complement(11117..12577)
                     /gene="phoQ"
                     /locus_tag="OO349_002829"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HCR5867830.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAQWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            complement(12577..13248)
                     /gene="phoP"
                     /locus_tag="OO349_002830"
     CDS             complement(12577..13248)
                     /gene="phoP"
                     /locus_tag="OO349_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HCR5867831.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            complement(13417..14787)
                     /gene="purB"
                     /locus_tag="OO349_002831"
     CDS             complement(13417..14787)
                     /gene="purB"
                     /locus_tag="OO349_002831"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HCR5867832.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQHRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            complement(14791..15432)
                     /gene="hflD"
                     /locus_tag="OO349_002832"
     CDS             complement(14791..15432)
                     /gene="hflD"
                     /locus_tag="OO349_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707061.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HCR5867833.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSCQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            complement(15468..16574)
                     /gene="mnmA"
                     /locus_tag="OO349_002833"
     CDS             complement(15468..16574)
                     /gene="mnmA"
                     /locus_tag="OO349_002833"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HCR5867834.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIKVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            complement(16628..17089)
                     /gene="nudJ"
                     /locus_tag="OO349_002834"
     CDS             complement(16628..17089)
                     /gene="nudJ"
                     /locus_tag="OO349_002834"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HCR5867835.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            complement(17099..17752)
                     /gene="rluE"
                     /locus_tag="OO349_002835"
     CDS             complement(17099..17752)
                     /gene="rluE"
                     /locus_tag="OO349_002835"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HCR5867836.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKHENQPTRVILF
                     NKPYDVLPQFTDETGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            17924..19174
                     /gene="icd"
                     /locus_tag="OO349_002836"
     CDS             17924..19174
                     /gene="icd"
                     /locus_tag="OO349_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HCR5867837.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
BASE COUNT         5014 a         4727 c         5159 g         4360 t
ORIGIN      
        1 taaagaacaa cacaccggag aataaaaata atgaaactta aatattctgg cttaactgcc
       61 agtggcaaca ctcaccctaa atttacgcgc ggtgatattt accgcgacca gtacggcggc
      121 atggtaacga ttaaaggcgt ggcagaacgg cgcatcacct accgccgtga aggttacgaa
      181 tatgattgcg tgatgcctgt ttatcaattc cggcgtgatt tcacgctggt acaggcagcg
      241 ccccgcagta aaccaaccag cagggagaaa gcacgcgcca atattcagga aataaaaaag
      301 atgcttaacg tattcagggg taaaaaatga aactggcacc gaacgtaaaa aaacagccac
      361 gcggaataaa acacaaagac acagaggtaa ttatctttgc gggtagtgat gcctggtcgc
      421 acgcgaaaca atggcaggag caggatggcc ccgcatccgg cgataatgtg ccgcctgtgt
      481 ggcttgggcc aaatcagctt gccgaacttg atgcactgaa aattgttccg gatggaaaaa
      541 aacgcgtaag gctgtaccag gccggagaac tggatttggt ggagaccaaa aagattggtc
      601 agaagctggc ggcggcagat attcaggacg caaattttta ccccgaagga atgcacgtcc
      661 agaagtgtga aaactggcgg cgctatctga atgctgagcg tgaaaatatt gccgcagggc
      721 ttaccatgcc ggagcagaaa aatacgcaac tggcacaaat ggcagacagt gagcgcgcgc
      781 agctgcttgc cgagcgcttt gatggcgttt gtgtgcatca ggaaagtgaa atcgttcacg
      841 tatggcgcgg cggggtatgg tgtccggtca gcacaatgga acttagccgc gaaatggtgg
      901 cgatctattc agagcacagg gccactttca gcaagcgcgt aatcaataac gccgtggaag
      961 cgttaaaagt tattgccgaa cccatgggcg agccgtccgg cgatttgctg ccgttcgcca
     1021 atggtgcgct tgacctgaaa acgggggaat tttccccgca cacgccggag aactggatca
     1081 ccacgcacaa cggcattgag tacacgccac cagcaccagg ggagaacatc cgcgataacg
     1141 cgctaaactt tcataaatgg cttgagcacg cagccggaaa agaccagcgc aagatgatgc
     1201 gtatatgtgc cgcgctgtac atgattatgg cgaaccggta cgactggcag atgtttattg
     1261 aggccaccgg agacggcggg agcggtaaaa gtacattcac acacatagcc agccttctgg
     1321 cagggaaaca gaacacggta agcgctgaaa tgacatcgct tgatgatgct ggtggacgtg
     1381 cgcaggttgt cgggagtcgt cttatcgtcc tggctgacca gccgaaatat acaggcgaag
     1441 gaacgggcat caagaaaatc acgggcggtg accccgcgga aattaacccg aaatacgaaa
     1501 agcgtttcac ggctgtaatc agggcggtgg tgctggcgac caacaataac ccgatgatat
     1561 tcaccgaacg ggccggaggt gtggcacgtc gtcgcgtgat tttccgtttc gacaatatcg
     1621 ttagtgaggc cgaaaaagac agggagctac cggaaaagat tgcggctgaa atccctgtca
     1681 ttatccgccg attgctggcg aactttaccg actctgagaa agcacgggct ttactactgg
     1741 aacagcgtga cggtgatgaa gctctggcaa taaagcaaca gacggatccg gttattgagt
     1801 tttgccagtt cctgaatttt ctggaggaag cacgcggcct gatgatgggc ggcggtggcg
     1861 attcagtgaa gtacacgacc agaaacagcc tttaccgcgt ctatctggcg tttatggcgt
     1921 acgcaggcag gagcaaaccg ctaaacgtaa atgactttgg caaggctatg aagccagccg
     1981 cgaaagttta cggacatgaa tatattacgc ggaaagttaa gggagtaacg cagactaacg
     2041 caataacaac agacgagtgc gacgcgtttt tataattttt tgtaattgct gtctaccctg
     2101 tctacctgag taaagaaaaa tacatttaat tcagtgcatt aagttaggta gatagccttt
     2161 ttttactgtc tacccactat ctaccctctc tacctgattt tacctgaatc agacagggag
     2221 gtagacacgg ggtagatagt ggataaaagc actctacccc gctgaaagca gcgccattac
     2281 tggcatgagg gccactaagg tagataaggt agacaagggg aggcacaact caaaactttt
     2341 taaacgaggg ggtaaaaata aatatgcaca cttcagggaa atttaataaa tcattcaaaa
     2401 aacgcagaga cagaacagaa ccgaaatatc gcgcgttaga catgacagag cacgctttaa
     2461 aggtggcaat cagaacgata gaccgccacg cgggggaagg atacgcgaaa gcacatcccg
     2521 aactgataag cgcattcatg accacgacgg cggcaaattt tgccacgctg acagagcggg
     2581 agattgccga agcggaacag gtaacaacaa tcaacgttaa aaccggagag gtggaatcat
     2641 gagcgaacca aaatttggtg aaaaatttta caagcacaac ggacggatca cgattctgca
     2701 aataagcgca gctacaccag gctggtgggt tgaaacagac gaaggatcct cacccgtggc
     2761 atcatgggcg ctttgtgtgc ttagttaccc tgaccgcgat gtgtatcagg atattctgcc
     2821 tgtgatatcc actgacaaag gcatgaaacc cgtagacatc aaaaacatgg ggtttcagtg
     2881 cgtaatgctc actgaaaaaa tgatggaaga gatgaaaaag aacaatgccg gatcgcttca
     2941 ctgaggtggc aatatgacag cacaaattgc agcttacggg cggctggtgg gcgacccgct
     3001 ggtaaaacag agcagcaagg gcacaccgga gccgccaacg gagaactacg cagacctgag
     3061 aaatgcagta aatcgcactt acgcagacat ggggctgagc aaattagcag agtaggaata
     3121 tcagaaccac accacgcagc cggagcaatc cggctttttt tgcgtcattt gaaacggtat
     3181 ggagcactac tggcttttcg tcacggtccg gcatagttac tatctgaaac aaacagacac
     3241 aacagaggaa aacgcaatgc cgatgaaatt tgatgagata cttaaacagc gtgataaata
     3301 ccatgctgac aacatggaga cgatgaatat cactgattac cgcgcatttc tggaaacggg
     3361 ggcgctgatt gaaaaggatc atcatggttt tgttcgttgc gcgctatccg gtgaaatgct
     3421 ggcagtaaat cctgaacaga cagatgcatt gatagaattt ctgaaaggga tcagagactg
     3481 agcacccata cagcacatac agccggagca atccggcttt ttgttatttt ttgtaaatta
     3541 tttgttcgtg gttgttccac gttgttcact gagcggatcg gcatatttta cccgaactga
     3601 atcatgatta ttctcgcccg tggtgccagg acgctggggc cactttcccg cctgttaatg
     3661 tgctcgccaa tattcattac caggcgggaa aacgatcggt gcgattactg atttccttat
     3721 gaaaaacggt tgagtgtttg ccgcgtcctg gagttcctta cttaacccca ggactttttt
     3781 tatgcagaga ataatcgaat tacgccagca gaaaaccgcc attaaaaatc agatgcgcga
     3841 catgctggag aacgctgaaa aagaaaaccg cagtcttaac gatgctgagg gcacaaaatt
     3901 tgacgaatta cgcgctaaag ctgaatccct cgataaagac atttcccgcc ttgaagccat
     3961 tgcagacgaa gagcgcagca agccaggtaa aagcagccag accactgacc ccgcagaact
     4021 acgcaactac attctgaccg gtgaaacccg cgcattaagt acaggcgttc ccgctgatgg
     4081 tggttatacc gttatccctg aactgaacac cgaaatcatg cgaatgctgg cggatgaatc
     4141 caccatgcgt cgcatctgta ccgtgaagaa aatcagcagc aacgagttta agcagcttgt
     4201 ttccgctggc ggtgcgaccg ttaaccacgg tgaagagggt aaggcacgcg aacagaccag
     4261 caccccgcag attaacgagg tgagcattaa gctgtatccg gtctatgcgt acccgcgcac
     4321 cacacaggaa atcgtggatt tttccgatgt ggacatcctt tcatggctga cgggtgagat
     4381 tggcgacacc ttcacggaaa ccgaagaaag cgatctggtt gtgggcgacg gtgacaaaaa
     4441 agcaaaaggt tttttatccg taccccgtgc agagaagaac gacaaagagc gtgattttgg
     4501 tacgttgcag gtaattaaac cttccgaatc tctggcgtgg acatctgcgg acccgctgat
     4561 cgacctgaaa tttgcattac gtaaaaaata ccgcaaaaac gcggtctgag tggttaactc
     4621 cacgacggcg gcaaaacttc agaaggtgaa gaacgcgaac ggtgattata tctggcgcga
     4681 ccgtttacag acgggtgatc ctgatacgtt gctgggcctt ccggtcgaat atctggagtt
     4741 tatgcctgat aacgttattg ccctgggtga cttcaaacgc ggttactaca ttgttgatca
     4801 cgaaacaggt gttcgcacca gaccggacaa cctgacagaa ccaggattca tcaaaatttt
     4861 cacgcaaaaa tatttaggcg gtggcgtggt ggattcgaac gcgatcaaga ttctggcact
     4921 gccggaagac gacgattaac agcatacaga aggggctaaa aagccccttt agtgttttat
     4981 gggtgaaaaa attatgaaga gtatggaaat ccggtcatcg gaaatcacca ccagcggagc
     5041 cggtacgctg acgggctacg ttgttcgctg ggataagctt tcagaactgt tatggggtga
     5101 gttttacgaa aaattccagc ggggggcgtt tactgaatgg cttgcggcgg gtaatgacgt
     5161 tcgcggcctg tatgagcatg accacagcat gttactgggg cgcacccgtt ccggcacgct
     5221 gaaactggaa gaggacgaaa cagggttacg ctttgaactg accccaccgg ataccagtac
     5281 agggcgcgac gttatcgaac tggttaagcg cggggacatc tcggggatga gctttggctt
     5341 tcgctcccgt aaggatgtat gggataccac aacagatcca tgcgtgcgca ccgtgctggt
     5401 ggcggaactg tacgaaatta ccgttacatc ggtaccggct taccccgatt ccggcgtgga
     5461 gctggcccgc cgttccctgt atgagcagca ccccgaaaaa atgccgcgtg cggataatcg
     5521 ccgctggtgg gcggatttag cgggggtgtg atatgtggcc tttcagacga aaaaaagagc
     5581 agcgcagcat gacgcttgat gaatttatgg cgctggccgg cacatcgaac acgggggcgg
     5641 gtgagtacgt atcatctggg acagcggaat cactccccgc cgtcatgaac gccgtcacgg
     5701 tcatctctga cgcggtggct accatgccgt gttacctgta cctggtacgc aatgagaagg
     5761 ggaaggaggc ccgcgaatgg cttgattctc atccggtcga tcacatcctc aacgagcgcc
     5821 cgaacgcgtg gcagacaccc taccagttta agcgaatgat gatccgctac tgcctgttaa
     5881 gtggtaatgc ttatgcggtt attcagtggg ggcgtgatgg ttttccggcg gctttacatc
     5941 cttacccgcc gcagtcggtg aacgtggagc agacaggtga tcataactgg cgctactgca
     6001 tcactgacgc ctacaccgga aacacccgca actatttacc gtgggaggtt ctccaccttc
     6061 gttactccac ggatgacggt tttatggggc gctcacctgt aaccatctgc cgcgaatcgc
     6121 tggggcttgg gctggcccaa caacgccacg gcgcgagcgt gatgcgtgat ggcatgatgg
     6181 cggcaggggt tatcacgtca ggcgaatggc tggacggcgt gaagggcaaa caggcattag
     6241 ccgcactgga acgctacaaa ggggccagaa acgccggaaa aacgcccatc cttgaagggg
     6301 ggatgagcta tcagcagctg ggcatgagta atcaggatgc cgaatggctg gcctcccgtc
     6361 gcttcaccat tgaagacatc gcccgaatgt tcaacgtctc gccgattttt ttgcaggaat
     6421 acagcaacag cacctacagc aacttcagcg aggcaagccg cgcatttctc accatgacga
     6481 tgcgcccgtg gctggcgaac tttgagcagc agataaaaaa cgccttgctg gtggcctcgc
     6541 ctgtacctgg tatccggtat caggtggagt ttgacagcgc ggacctgtta cgggccacac
     6601 ctggcgaacg ctttgccacc tatgagcgcg gcataaaatc cgacgttatg tgtccgaacg
     6661 aagcccgcga acgtgaaggg ctgtcaccgc gtgatggtgg tgatgagttc agccaggcat
     6721 ggaaacagga agtaaaaatc agcgagggag aaaaaccgga atgaacatag ggcgactgcg
     6781 tgacaggata acgattcaga ccctgaaaca gaccagggat ataaccggcg aaatactcga
     6841 aacgtgggag gacggtcaca cactctgggc aagcgtgaac atggtcagca gcaaggaggc
     6901 catttcatcg ggcgcagagc tggcgattgg taccgtaagg atctggatac ggtaccggaa
     6961 ggacatcaac gccaccagcc ggataaaggt cagtacgggg ccgctggcgg ggcgtgtact
     7021 gaatatcatc gggcagccgc tgccggatgc cgccaggaca cgccttgaaa ttctttgtcg
     7081 tgagggcgcg gaaaaatgac agaagaactt atcaccctgg aagaagtgaa actccattgc
     7141 cgcatcgatg gcgacgatga agaccagtta atcagcggat acattgccgc atcgcttgag
     7201 gcgtgccaga tacacatagg caggcgcttt gatgacgggc tggcgttcac gccagccata
     7261 aagattggct gcatgatgtt tatcgctcac ctgtatgaaa atcgccagat tgtcgcggat
     7321 aacgcaaaaa cacgcgtacc catgacgata ggcgcactct ggacggctta ccgtgatgtg
     7381 ggggggtact gatgccgtgg caaccattaa gacgatgcac agaaccaggc tgtaacaggc
     7441 gcgttaaatc cggcaagtgt gaggagcaca ggcgggcggc atggcgtgca gaggatgcca
     7501 gacgaggaca ccgccgcgcg cgtggatatt ccagacagtg ggacaaatac cgcgccatgt
     7561 atctgagtaa aaacccgtta tgcgtgcgat gccttgagaa ggggatatat acgcccgccg
     7621 tggtggtgga tcacattatc ccgattaatg gcggtgatga tgttctcttc tggcccgaat
     7681 ggaaccatca accattgtgc caggcgtgtc ataaccagaa aacgaaatgg cttgatccgg
     7741 caacaaaaag caagcgtgcc gcaggtggat ttcgtgaaga ggaagaacgg gctgctaacc
     7801 gcaataactg gatgtatgac gctgatgaat gagcgggaac aaaaccgcct tatccgtgga
     7861 cttataaggc agcgtgacgc atggaagaca caggaaacag ggcataaaga taaagcgtca
     7921 ggacgcgcag aacgcatcac agcgaagcga ttaaccgacc gtgaccgcga ggttatggaa
     7981 tgtttccgca atcgctgatg aggctgtatg acggggtggg gggcgttttc aggacaaacc
     8041 cgaccccgcc tacctggatg atatcgccgt gaagcagtgg cgggaaaaat cgcggcagct
     8101 tgcggaacag ggagacctga cccccgccga ctggagcaat ctggaactgt attgcgtcaa
     8161 ctactccatt taccggaaag ccgttgcaga ccttgcggcg cgcgggttca gcattgttaa
     8221 cagtcagggc ggcgagagca gaaaccccgc attaagcgca aaatccgacg ctgaaagagt
     8281 gatgataaaa atggcctcct tgctcggttt tgacccgata agccgccgta aaaatccacc
     8341 gtaaacagaa gaagaggacg agcttgaccg cctggaataa gtacgcagaa gacgtaaaaa
     8401 cgggcaaaat tccggcctgt aaacggttaa aacaggctgt taaacggtac ttttcggacc
     8461 ttaaaagccc cctttacacg ttcgatcgtg aggttgtgga gcggtttatt gccttttcca
     8521 gggtgtgccc gcacgtaaaa ggaccgatgc gtggcagacc cattgagctg gagccgtggc
     8581 agcagttcgc ctttgcgtgc atcctcggct ttaaggttaa ggccaccgga cggcgcaaat
     8641 acacgagcgc ctttatcgaa gtgccgcgca aaaatgcgaa atccacgacc gccgcgattc
     8701 tggctaactg gtttctgatt atggagaacg gtcagcagga tatctacacc gcagcggtga
     8761 gccgtgacca ggcgcggatc gtgtttgata atgcgcgtca gatgtgcctt ttatcccgac
     8821 cgttacgcag gcgggtgaat attcaggcgc ataaggtgat acacccgaaa agcaacagcc
     8881 tgttaaagcc gctggcagca aaagcggcaa ccatcgaggg gacaaacccc agtctttcca
     8941 tcgtggacga ataccaccta caccccgata acggcgttta ttccgcgctt gagctgggga
     9001 tgggcgcacg tcccgaaggg ctgttatttg ccattaccac atcgggcagt aacgtcgttt
     9061 cagcctgtaa acagcactat gattattgct gccagatcct ggacggcgaa gaggtcaacg
     9121 attcaatttt tgtactgatt tacgaactgg acgacgaaaa cgaggttgat gatccggcga
     9181 tgtggataaa ggcgaaccct aatattgatg tttccgtgga tcgtgaaaaa ctggcctcaa
     9241 ccatccagaa agcgcggggt attccgtcgc agtgggtgga gatgatgaca aagcgattta
     9301 atatctggtg tcaggggact accccgtgga tgggtaatgg tgcatgggct gagtgtgccg
     9361 gaacgttcac ggaggaagat ttacacggtc aggagtgtta cgcggggctg gacttatcat
     9421 caaccagcga tatttccagc gtatgctatg cctttccggt cggtaaaacc attatgctga
     9481 tttcccgtca ttatctgccg gagttccagc tacagaaccc cgccaataaa aaccgcgctg
     9541 tctatcgcca gtgggctaaa gcgggctgga tacgcacaac gccaggtgac tgcattgatt
     9601 acgaccggat cagagatgac atcatgcagg atgcggagaa atttaatatc aggctggtgg
     9661 gctttgatac gtggaacgcc acgcatctca ggacgcaatt acagggggct ggttttgagg
     9721 tggagccgtt cccgcaaacc tacctcagat tcagtccggc ggcgaaatcg ttcgaagttt
     9781 ttgttaaccg cagggtgatt gtgcatcgtg gcgatccggt gttgtcttgg tcgatgagta
     9841 acgtcgtgat gcagagtgac gcgaacgcca atatcaagcc gaacaagaaa aaatcaccga
     9901 acaagataga cccgagcgta gcggcgctga tggcgtttgg tacattccag gcagagcatg
     9961 aggattttgc ttttgatata agcgacagcc accgccagaa actggaagaa tttagcgggg
    10021 tgtaatgagg tcagcagcat gacagaagcc gaaatactgc gattaatccg ccgcgtctct
    10081 ggaatcagcc agcaggctga cgaacagacc acgcagccgg acagcgtgac agccgaaaat
    10141 tatgcgcgtg ttgttgctga ggtgatgcgc cgtgatggta tccagcttaa tgatgcggat
    10201 atgcgcgaca tacggatccg cgttctggaa atgctggcct acaatcgccg cgttgaactg
    10261 tatcgggaga aggagaaaat cacgtaccac tggaagaagc cggagcggtt acggcggtaa
    10321 ctcactgaga ttaaagcaaa gcgcaaaatt gcgctggcta catccctcac atctgaggac
    10381 tgttaaccag caggccggaa tatattcaga aattacgaaa ttttcgcagt ttaattactc
    10441 gtcagcttaa tcgctaaccc gctctgatat tttcggaaac ctcaatttga ggaagtcggc
    10501 gaggtaactg tttgatattc ccgatagcgc aaaaatgcgc ggtcgggcag aatgaccacc
    10561 acctcaatcg tgacggagag tgacaaccag tgacacaccg tgacaaaaag acggtttaac
    10621 cgttcattaa aaatcgacgt gattttgtgc tttccggatg acgtggccaa catcattttt
    10681 tagcgaaaaa ttctgattaa ggtcactggc tgaacggtca taatgaccac gcagaccatc
    10741 gaggcaacca tagcgatcat cgttacggtt ggagcttttc cgccattggt ggaaaaggtg
    10801 cacatctgga tcatcattac ggtgatcaag ggtatgtaaa acccaccagc ctgattaaag
    10861 gttaaccgga aaaaaaatcc ggtagccccg ttatggggat ccccatatcg acattaacgc
    10921 ccctcatgaa ttggtgcgct tccccctgga aagattaccc gcctgtatat ttcttgtgtc
    10981 tatttgttcc acgttgtttc atacagtgca ccgaacggtg tagttaatgg tgtagtcaat
    11041 ttgcgatttt tggcactttt tcaatgaaaa ttgtttgatc gaatcaatga cttgatgtag
    11101 tggtaaaagg acatatttat tcatctttcg gcgcagaatg ctggcgacca aaaatcacct
    11161 ccatccgcgc accgcccagc atgctctctc cggcgacgat tttaccctca tattgctcgg
    11221 tgatttcgcg ggctaccgcc agccctacac cttgcccagg gcgtaaagta tcaacccgtt
    11281 gaccacggtc gaaaatgacc tctcgcttgc ttaatggaat accggggcca tcatcctcga
    11341 ccacaatata gagatgctcg tcggtttgcc ttgcagaaat ttcgacaaac tcgaggcaat
    11401 atttacaggc attatccagc acgttgccca tcacctcgac aaaatcgttc tgctcaccga
    11461 caaagctgat ctctggcgaa atatcgagag agatattgac ccctttgcgt tgatacactt
    11521 tgttcagcgc tgaggtgaga ttgtccagca gtggggcgac cggatgcagc tcgcggctga
    11581 gcaatgtccc gccgcgcata ctggcacgat gcaggtagta gccaatttgc tgtgaaatgc
    11641 ggctgatttg ctccagcatt accggctcag catcactgac gctcatcttt tcactacgca
    11701 gagaacgcag cgtactttgc agcaccgcca gtggcgtttt cagactatgg gtcaggtcgg
    11761 tgagcgtcgt acggtatttg tcgtaacgtt cgcgttcact ttttaacaat cggttcaggt
    11821 ttcgtaccag actggtcagt tctcgcgttg tggctggatt gagcaattcg cggttatgtt
    11881 cttccagttc gcggacttct tttgccaggg cttcgatggg gcgtaaactc caccaggcgg
    11941 cgacccacag cagcgggatc actaacagca gattggctga gagcacatag ataaaccagc
    12001 tccagaccat ataggaactt tttagctcca ccggaatggt atccaccacc acaatggtta
    12061 attttggcat ccgcgatgtt gccgggtaga cgtttaccgc caccgagtgg gtcatctccg
    12121 cgtcgtcatc atcttcccgc acttcctgca actgttgctg tatcgaatga tctccactca
    12181 gcaagaggct ggtatcgtta acatccgctt caatttcatg aaaaccattc gatttcagcc
    12241 agtcaggctg gatcattttc atcagccagg gcacgtcacg ttgcgcccat aaaagctgcc
    12301 cgttctcatc ataaattagc gtcatggtgg ggctttgctt gtcgatattt tcgggtaact
    12361 cgacatgcaa cttattgttt tcccactgcg caagggtata gaacagattg ctctcgccac
    12421 gtaacagccg aaacgtagtt ttatcgaaac tgacgctata accgatcagc gcgaccattc
    12481 cgtaggcaag cgaaagcacc agtactaccg ctgccgttgc caacagaaaa cgtacccgca
    12541 gcgagagcgg gaaaaaaaga cgcagtaatt ttttcatcag cgcaattcga acaggtagcc
    12601 ctggccgcga acggtggtaa tgacttcttg cggatattgt gcctgaattt ttttgcgcag
    12661 acgtcccatc agtacatcaa tggtatggct ttcccgcagc tccgcatccg gatagagttg
    12721 tagcattaac gaatctttgc tgaccacttt gccattattg cgtatcaacg tttccataat
    12781 ggtgtattcg aacgcggtca gtttgatcac ttcgtcatta atagataatt cacggcgaga
    12841 gagatcaacc tgaaacgggg ggagcgaaat gacctgtgaa gccagaccgc tattacgccg
    12901 cattaatgcc tgcattcgcg ccatcacctc ttcaatatga aacggtttag tcacgtaatc
    12961 atcagcaccg gcacttaata cttcgacttt gtcctgccag ctttcacggg cggttaatac
    13021 cagaatcggc agtgaaacat cgttgctacg ccagcggcga atcagtgaca gaccgtcctc
    13081 gtcaggcaat ccgagatcga caatcgcaat atccggtaaa tgttcattga gataataatc
    13141 ggcttctttg gcatcttctg cgtcatcgac ctgatgacca gcatcctgaa tctgaacttt
    13201 aaggtggtga cgtaacaacg cattgtcttc aacaaccagt acgcgcattt ttatttctcc
    13261 ctgtcttaat tattaaaata gtgtaacgcg attatgtgtt tatgggggta aacattaaat
    13321 aaaccagcgg ggaggggagg taaagtgaaa aaagaaaaag tggataatct taataagcag
    13381 gccggacagc atcgccatcc ggcactgatt tgaggcttat ttcagctcat caaccatcgt
    13441 gatagctcga ccaatatagt tagccggcgt catcgctttc aggcgggctt tctcttcttc
    13501 tggcaacgcc agaccatcga taaactgctt catgccttcg gcgtcaacgc gcttaccgcg
    13561 agtaagctct ttcagcttct cgtacggttt ttcgatgcca tagcgacgca taactgtctg
    13621 gattggttct gccagcactt cccagttgtg atccagctca tccagcagat ggtcacggtt
    13681 cacttccagt ttgctcacgc ctttcagggt ggattgatac gcaatcagcg cataaccgat
    13741 acccacgccg aggttacgca gcacggtgga gtcggtcagg tcacgctgcc agcgggaaac
    13801 cggcagtttg cttgccagat gctgcaatac cgcgttggaa aggcccagat tcccttcgga
    13861 gttttcgaag tcgatcgggt taactttatg cggcatggtg gaagaaccaa tctcaccagc
    13921 aatggttttc tgtttgaagt ggttaagggc gatataaccc cagacgtcac ggtcaaagtc
    13981 gatcagaatg gtgttgaagc gcgcaacgca atcaaacagt tcggcaatgt agtcgtgcgg
    14041 ttcaatctgg gtagtgtacg ggttccattg aatacccagc gaggtgacga actcttcgct
    14101 gaactgatgc cagtcaactt ccgggtaagc ggcgatgtgg gcgttatagt taccgaccgc
    14161 gccgttgatt ttgccgagga tctccacctg gttaagctgg cggtgctggc gctccatacg
    14221 gtaggcgacg tttgccatct ctttaccgat ggttgacggc gtggctggct gaccgtgggt
    14281 acgagacagc agcgggatat cgcggtactg aacggcgaga tctttaatgc catcaatcag
    14341 ttgacgccag tacggcagga tcacttcatc acgcgcggtt ttcagcatta atgcgtggga
    14401 gaggttattg atatcttccg aagtacaggc aaagtggatg aattcagaaa ccgcgtgcag
    14461 ttccgggatc tccgccactt tttctttcag gaaatactca accgctttaa cgtcgtggtt
    14521 agtggtacgc tcgatggttt tgatgcgtgc ggcatcttct tcgctgaaat tggcgacaat
    14581 tgcatcaagg taaccgattg cgtcggcagc aaaagcagga acttccttga tcgctgcgtg
    14641 cgcggccagt ttttgcagcc aacgtacttc aacttgtaca cggaatttca gcaaaccata
    14701 ttcgctgaaa atcccgcgca gcgcgctgac tttatcgccg tagcgtccat cgacagggga
    14761 aacggcggtc agtgaggata attccataga tcacaactcc ggggttaaat gagcaagaat
    14821 ttgttttgcc tgagtggtca ggcgattacg agaaaacatc agttgtagac gtccgccgcc
    14881 gacctggtgc cagagcacgg cggcgcgaat gcctgccagc agggttgcgc gaactttcgc
    14941 ctgcacttgt gggctttgca gtacagcagg ggaaccggtg acctgaatgc gcgggccaag
    15001 cgggctgatc acatcaacat agatagcagc catcgcgctc atcagcgttt cggactgtaa
    15061 atcgaagtgt tcgagctggc gttgcaggcc gttgatccgg ttgcccagag tgtcgagcgc
    15121 gcctttcgct gaggagagtt tgcgctcaag caccatcaag ctgagtgtgt agcgggttaa
    15181 ttcggcgttt aagccctggc aactgctggc attgagcacg ccgagcaggg tttccagccc
    15241 gacgcgcagg ttggcttcgc taccgccaaa aaccgccagc gtcgagctgg ggttcatatc
    15301 aataatactg ttgagtgaga cgtgtagcgc atcggcatca caatgcccct ggtgagcgag
    15361 ttgttgcacc aggcgtgccg actgacaaat accggccagg gcgagggtga tgtcatagta
    15421 attctttgcc acgttcactg cttccttgtt ttaagtaaag ataataatca gaccggcagc
    15481 ggcagacgct gctcaataat accgccaccg aggcacactt caccgttata gaagacggca
    15541 gactggcccg gcgtcacggc ggcaaccggc tcatcgaaaa tcactttaat gcgatcgtcg
    15601 tccagcgcct tgacggtgca cggaatgtcg gtctggcgat agcgggtttt taccgtgcaa
    15661 cgcatagtgc cggtgaatgg ttcgcgatcg acccagtgca gctgctgggc aatcaacccg
    15721 acagacatca gccgcgggtg ttcatggccc tgagcgacaa ccagaatgtt gttttcgacg
    15781 tctttgtcca ccacatacca cggttcttcg gtgccttctt tggtgccacc gatacccaga
    15841 cctttacgct gaccgagagt gtgatacatc agcccctggt gctcaccaat ttcatcgcca
    15901 tcgacggtaa tgattttgcc cggttgcgcc gggagataac ggcccaggaa ctcgcggaat
    15961 ttacgttcgc caatgaagca aatgccggta gagtcttttt tcttcgcggt gaccagacca
    16021 agatcttcag caatcttacg cacctgcggt ttttccagtt cgcctaccgg gaacaggctt
    16081 tgcgcaatct gctcatggct gagcgtataa aggaagtagc tctggtcttt attgctgtcc
    16141 agaccacgca gcaggcggct cttgccatcg acatcggccc gacgtacgta atgaccggta
    16201 gcgatataat cggcacctaa atcttcggcg gcaaattcga ggaaggcttt aaatttgatc
    16261 tccttgttgc acagaatatc cggattcggc gtgcgaccgg ctttatattc ggcaaggaac
    16321 agttcgaaga cgttgtccca gtactcggca gcaaagttaa cggtgtgcag ttcaatgccg
    16381 agcttgtcgc agaccgcctg ggcatcagcc agatccgctg ccgctgtgca atattcctca
    16441 ccgtcgtctt cttcccagtt cttcataaac aggccttcga cctgatatcc ctgttgttgc
    16501 aacagccagg cagaaacgga ggaatcgaca ccgccggaca tgccgacgat tacttttttt
    16561 gcggtttcag acattggatc actcacgaca ttgaacttca aggcggcgta ttctatcacg
    16621 cagcctttta gatgacaccc tttgtaaaag gccagttaaa atcgccaatc atctccagcg
    16681 gataacgttg cccgctttga taacaacgaa tactttccgc caccagcggc gaacgaagat
    16741 ttgacgcctg taaaatttct tcggcgctga cccaacggca gcagtcgata tcgctgtcat
    16801 gaggctgggt cgggcatatt tgctcaagct caatggcaaa gaggaaacgc aaaaacggcg
    16861 ttttatctgg cgcaatccac tgatgcatac gaataaagtg ttgcggctgc gcgctgatgc
    16921 cggtttcttc ccacagttca cgggcggcgg cttccactaa ggtttcatcg gcttccagat
    16981 gcccggcagg ttggttccat aacgctttac cattaatcgt ctcttcaacg actaaaaatt
    17041 tgccttctgc gtgcaccacg caagcaacgg taacgtgcgg tttaaacatt ttgcatcctt
    17101 aatctgtcac ttctcgccat tcgccattgg caagattatc caaagagtaa tctcccatcg
    17161 catagcgaat cagtcgcagc gtggggaaac caacatgggc ggtcatgcgg cgcacctggc
    17221 gattacgtcc ttcatataag gtgatcttca gccagctggt gggaatactt ttgcgttcac
    17281 gaattggtgg attccgcggc cataaccacg cgggttcgtc aaccagctcc gcgcctgcgg
    17341 gcagggtagg gccatcattt aaggttacgc cattgcgcaa ggcttcaagt gcgtcttgtg
    17401 tgggaatacc ttccacctgc acataataga tttttccggt gcgtttaccc ggctgggtta
    17461 aacgcgcctg caacgcgccg ttattggtca gcaccagcaa cccttcgcta tcgcggtcaa
    17521 ggcgacctgc tgcataaacg ccctgaaccg ggatgaattc ttttaatgtt ttgcgtccgg
    17581 tttcatcggt gaactgcgga agaacatcgt agggtttatt gaagaggatc acacgcgtgg
    17641 gctggttttc atgtttacgc ctggtagaac gttgtgagct gaatcgctta acctggtgat
    17701 ttctaaaaga agttttttgc atggtatttt cagagattat gaattgccgc attatagcct
    17761 aataacgcgc atctttcatg acggcaaaca atagggtagt attgacaagt caattacaaa
    17821 tcattaacaa aaaattgctc taaagcatcc gtatcgcagg acgcaaacgc atatgcaacg
    17881 tggtggcaga cgagcaaacc agtagcgctc gaaggagagg tgaatggaaa gtaaagtagt
    17941 tgttccggca caaggcaaga agatcaccct gcaaaacggc aaactcaacg ttcctgaaaa
    18001 tccgattatc ccttacattg aaggtgatgg aatcggtgta gatgtaaccc cagccatgct
    18061 gaaagtggtc gacgctgcag tcgagaaagc ctataaaggc gagcgtaaaa tctcctggat
    18121 ggaaatttac accggtgaaa aatccacaca ggtttatggt caggatgtct ggctgcctgc
    18181 tgaaaccctt gatctgattc gtgaatatcg cgttgccatt aaaggcccgc tgaccactcc
    18241 ggttggtggc ggtattcgtt ctctcaacgt tgccctgcgt caggaactgg atctctacat
    18301 ctgcctgcgt ccggtacgtt actatcaggg cactccaagc ccggttaaac accctgaact
    18361 gaccgatatg gttatcttcc gtgaaaactc ggaagacatt tatgcgggta tcgaatggaa
    18421 agctgactct gccgacgccg agaaagtgat taaattcctg cgtgaagaga tgggcgtgaa
    18481 gaaaattcgc ttcccggaac attgcggtat cggtattaag ccgtgttctg aagaaggcac
    18541 caaacgtctg gttcgtgcag cgatcgaata cgcaattgct aacgatcgtg actctgtgac
    18601 cctggtgcac aaaggcaaca tcatgaagtt caccgaaggc gcgtttaaag actggggcta
    18661 ccagctggcg cgtgaagaat ttggcggtga actgatcgac ggcggcccgt ggctgaaagt
    18721 taaaaacccg aataccggca aagagatcgt cattaaagac gtgattgctg atgcattcct
    18781 gcaacagatc ctgctgcgtc cggctgaata tgatgttatc gcctgtatga acctgaacgg
    18841 tgactacatt tctgacgctc tggcagcgca ggttggcggt atcggtatcg cccctggagc
    18901 aaacatcggt gacgaatgcg ccctgtttga agccacccac ggtactgcgc cgaaatacgc
    18961 cggtcaggac aaagtaaacc ctggctctat tattctctcc gctgagatga tgttacgcca
    19021 tatgggctgg actgaagccg cagacctgat tgttaaaggt atggaaggcg cgatcaacgc
    19081 caagaccgta acctatgact tcgagcgtct gatggaaggc gctaaactgc tgaaatgttc
    19141 agagtttggt gatgcgatca tcaagaatat gtaatcacta catgtgttaa atatttaaac
    19201 gggcgtataa cacgcccgtt gttttattta tgtggatatt attaatagca tatcgagcat
//
LOCUS       DAJTQY010000078        17396 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.078, whole genome shotgun sequence.
ACCESSION   DAJTQY010000078 DAJTQY010000000
VERSION     DAJTQY010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17396
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.078"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(44..775)
                     /gene="rlmB"
                     /locus_tag="OO349_002837"
     CDS             complement(44..775)
                     /gene="rlmB"
                     /locus_tag="OO349_002837"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HCR5867838.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(955..3396)
                     /gene="rnr"
                     /locus_tag="OO349_002838"
     CDS             complement(955..3396)
                     /gene="rnr"
                     /locus_tag="OO349_002838"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HCR5867839.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3435..3860)
                     /gene="nsrR"
                     /locus_tag="OO349_002839"
     CDS             complement(3435..3860)
                     /gene="nsrR"
                     /locus_tag="OO349_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HCR5867840.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4065..5363)
                     /gene="purA"
                     /locus_tag="OO349_002840"
     CDS             complement(4065..5363)
                     /gene="purA"
                     /locus_tag="OO349_002840"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HCR5867841.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPAELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5467..5664)
                     /locus_tag="OO349_002841"
     CDS             complement(5467..5664)
                     /locus_tag="OO349_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HCR5867842.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5746..6750)
                     /gene="hflC"
                     /locus_tag="OO349_002842"
     CDS             complement(5746..6750)
                     /gene="hflC"
                     /locus_tag="OO349_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HCR5867843.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6753..8012)
                     /gene="hflK"
                     /locus_tag="OO349_002843"
     CDS             complement(6753..8012)
                     /gene="hflK"
                     /locus_tag="OO349_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HCR5867844.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8098..9378)
                     /gene="hflX"
                     /locus_tag="OO349_002844"
     CDS             complement(8098..9378)
                     /gene="hflX"
                     /locus_tag="OO349_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HCR5867845.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKAMGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRACTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9454..9762)
                     /gene="hfq"
                     /locus_tag="OO349_002845"
     CDS             complement(9454..9762)
                     /gene="hfq"
                     /locus_tag="OO349_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HCR5867846.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9848..10798)
                     /gene="miaA"
                     /locus_tag="OO349_002846"
     CDS             complement(9848..10798)
                     /gene="miaA"
                     /locus_tag="OO349_002846"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HCR5867847.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMVIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10791..12638)
                     /gene="mutL"
                     /locus_tag="OO349_002847"
     CDS             complement(10791..12638)
                     /gene="mutL"
                     /locus_tag="OO349_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HCR5867848.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFSHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAAREPVAPRYSPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12648..13985)
                     /gene="amiB"
                     /locus_tag="OO349_002848"
     CDS             complement(12648..13985)
                     /gene="amiB"
                     /locus_tag="OO349_002848"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HCR5867849.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVAHKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQYEKLSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14004..14465)
                     /gene="tsaE"
                     /locus_tag="OO349_002849"
     CDS             complement(14004..14465)
                     /gene="tsaE"
                     /locus_tag="OO349_002849"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HCR5867850.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14437..15984)
                     /gene="nnr"
                     /locus_tag="OO349_002850"
     CDS             complement(14437..15984)
                     /gene="nnr"
                     /locus_tag="OO349_002850"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HCR5867851.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKLGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPSEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            15983..17122
                     /gene="queG"
                     /locus_tag="OO349_002851"
     CDS             15983..17122
                     /gene="queG"
                     /locus_tag="OO349_002851"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HCR5867852.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGALPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17105..17158)
                     /locus_tag="OO349_002852"
     CDS             complement(17105..17158)
                     /locus_tag="OO349_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5867853.1"
                     /translation="MRFYFYSQAVDESGVTR"
BASE COUNT         3946 a         4960 c         4370 g         4120 t
ORIGIN      
        1 aaaaaccatc caaatctgga tggcttttca taattcagag gaactagctg cgctgacgaa
       61 ccgcttcaaa taagcaaatt ccggttgcaa ccgaaacgtt cagggaagaa acacttcctg
      121 ccattgggat gctgatcaac tcatcgcaat gttcacgggt caggcgacgc ataccttcac
      181 cttccgcgcc catcaccagc gccaggcgtc cggtcatttt gctctgatag agtgtatgat
      241 ccgcctcgcc tgccgtaccg acgatccaga tattctcttc ctgcaacata cgcatggtgc
      301 gcgcaaggtt agtcacccga atcagcggaa cgctttctgc cgcgccgcag gctacttttt
      361 tcgccgtagc gttgagctgt gcggagcgat ctttcggcac aatcaccgca tgaacgcctg
      421 cggcgtccgc actacgcagg cacgcgccga ggttgtgcgg atcggttacg ccgtcgagga
      481 tcagcaggaa cggttgatcg agcgaagcga tcagatccgg cagatcgttt tcctgatact
      541 gacgtcctgg cttcacgcgg gcgataatgc cctgatgcac ggcaccgtcg cttttctcgt
      601 cgagatattg gcggtttgcc aactggataa ccacgccctg agactcaagg gcgtgaatca
      661 gcggtaacag acgtttatct tcacggcctt ttaaaataaa gacttcctga aaacgttcag
      721 gggcgcgctc cagcagggcc tgcactgcgt ggatgccgta aatcatttcg ctcattaatg
      781 tactcgttgt ttacgttttg cctgatgcac tacgtttatc cggcctacat gatctctgca
      841 atatattgaa tttgcgtgat tatgtaggcc ggataaggcg ttcacgccgc atccggcatg
      901 aacaaagcgc attttgtcag caatctaacc ctcttttttt aaagagggta ttgatcactc
      961 tgccactttt ttcttcgccg cacgcttcgc tttggtcgct gcggctattt tctgcgtttt
     1021 cgccgatggc tttttcgctt ttctcgcgtc tttcttcgcc gctttcggct tcgttttttt
     1081 ctcaccgcgg aaggcgctgt ctggctcaaa gtttaccttt ttaccgacct gacgacgctt
     1141 gccgcctttt ttgcctgcat cgcctttttt cgctttctcg cgcgccgttt taccgacgtt
     1201 gcgcggtgcg cgttcactgg agatcagact aaagtcgatt ttgcgctcgt ccatattaac
     1261 cgcttcgacg cgaacttcca cgcgatcgcc caggcgataa gtctggccgc tggattcccc
     1321 catcaggcgt tgccctacct ggtcaaagcg atagtagtca ttgtccagcg aagagacatg
     1381 gaccagacca tcaatgaaca agtcgtccag acggacgaag aagccaaagc cagtgacgct
     1441 ggaaattacg cctttaaaga cgttacctac ctggtcgagc atgaagtcac acttcagcca
     1501 gtcagcgaca tcgcgcgttg cttcgtcggc acgacgttcc gccatcgaac agtgctgacc
     1561 cagttgcaac atctcttcca tcgaataatg gtagccgccg gtttcagtgg tgttgccctg
     1621 atgcccctgc tctttcgcca gcagatattt aatggcgcgg tgcagcgtca ggtcaggata
     1681 acgacgaatc ggcgaagtaa agtgcgcata ggactgcaat gccagaccga agtgaccacg
     1741 gttttctgga tcgtaaatcg cctgtttcat cgagcgtagc agcatggttt gcagcatttc
     1801 tgcatcagga cggtcggcaa ccgactccag cagttccgcg tagtcacgcg gttccggctt
     1861 gttaccaccc ggcagctcca gccccagctc cgccagcact gaacggaaag aggtaatcgc
     1921 ttcggtgctc ggcttgtcgt gaatacggaa cagtgccggt tctttcgctt tctcaacgaa
     1981 acgcgccgcc gagatattcg ccagaatcat gcactcttca attaatttgt gcgcgtcgtt
     2041 acgctgggtc tgttcgatac gttcaatacg gcgttcagcg ttgaaaatga acttcgcttc
     2101 ttcgctctca aatgagatcc caccgcgttc ttcacgggct ttatccagca ctttatagag
     2161 gttatgcaac tcttcgagat gcttaaccag cggggcgtac tgctcgcgca gatcctgatc
     2221 gccctgcaga atatgccaga ctttggtgta ggtcagacgc gcgtgagagc tcatcaccgc
     2281 ttcgtagaat ttgtagcccg tcaggcggcc tttcgacgaa accgtcatct cgcacaccat
     2341 acacaggcgg tctacctgcg ggttgagcga acacaggccg ttagagagca cttccggcag
     2401 catcgggata acctgcgaag ggaagtacac cgacgtgcca cggttacgcg cttctctgtc
     2461 cagcggcgtt ggcggacgca catagtagct gacgtcggca atcgcgaccc ataaacgcca
     2521 gccgccgccg cgttttttct cgcagtaaac tgcatcgtca aagtcacggg cgtcttcgcc
     2581 atcaatggtg accagcggta aatcgcgcaa atcgacacgg cccgcttttg cttcttccgg
     2641 cacttcttct ttcagccccg caacctgttg ctcaacagcc tgcggccaga tatacggaat
     2701 ttcatgggta cgcagagcga tatcaaccgc catgccggtg cccatattgt cgcccagcac
     2761 ttcgacgatt ttacccaccg ctttggtgcg gcgagtcgga cgctgagtca gttcgactac
     2821 gaccacaaag cccatccgcg cgcccatgat ctgatcgggc gggattaaga tatcgaagct
     2881 cagacggcta tcgtcaggaa ccacaaagcc gacgcccgct tcggtaaagt agcgaccaac
     2941 aatctggctg gtttttggca ccagtacgcg gacaatacgc gcttcacgac gacctttacg
     3001 gtcagcaccc agcggctgcg ccagcacctg atcgccatga atgcaggttt tcatctgctc
     3061 gctggagaga tacaaatcat ctttacgccc ttcaacccgc agaaagccgt agccatcacg
     3121 gtgaccaata acggtacctt tcaccaggtc gaggcgttcc ggcagcgcat agcactgacg
     3181 acgagtgaag accagttgac catcgcgctc catcgcgcgc aggcggcgac gcaggccttc
     3241 aagctgctct tcgccttcaa tgtgcagttc taccgccagc tcatcacggc tggccggttt
     3301 ttcacgtttg gttaaatgtt cgaggataaa ttcccgacta gggatgggat tcgcgtattt
     3361 ttcagcttcg cgttcctgga aaggatcttg tgacatctcg gttcctccgt tgtcatctct
     3421 gatgaagatt ttcgtcactc caccagcaat aatttataaa gcggttgatt ctcttcaacc
     3481 aaatcggcaa gcgtgtagtt atccagttcc gtaagaaaac tttgcacggc cttagaaagc
     3541 gcctgtttca accgacaggc aggtgtaatg tggcaaaact cactgctgca attcaccagc
     3601 gataagggct ccagctcgcg caccacatca ccaatacgta tcgcactcgc cggtttaccc
     3661 aggcgaatgc cgccattttt tccacgaaca gcagtcacgt agccggcacg actaagttga
     3721 ttgattattt tgaccatatg attacgggag acgccgtaga cgtcagtcac ttcagaaata
     3781 ctggtcatcc gcccttctgg caatgacgcc atgtagatca gcgcacgtaa tccgtaatca
     3841 gtgaaactcg ttaactgcac atcaacctca aaagggaaat cgggaaaaaa tacatttata
     3901 ttgatgataa accagccaca agctgtgtcg ctaatttatt tcagttcggg gaaggaaaaa
     3961 agcgaggatt taacactgtg tcggatagcg taatggctta accgacacag caatgtatca
     4021 cactgttagc ccggcaggca aaatatctgc caggcgtacc agaattacgc gtcgaacggg
     4081 tcgcgcagaa tcatggtttc agtacgatcc ggaccggtag agatgatatc gatcggcaca
     4141 ccggtcagct cttcaatacg cttgatgtag ttcagcgccg cctgcggcag gccgctacga
     4201 tctttcacgc cgaaagtgga ttcagaccag cccggcatgg tttcgtaaat cggctctaca
     4261 cctttccagt cgtcagctgc cagcggagtg gtagtcactt cgcgaccatc cggcatacgg
     4321 taagccacgc agagtttaac ctctttcagg ccatccagaa cgtccagttt agtcaggcag
     4381 aagccagaca gggagttcag ctgtaccgca cgacgaacgg caacggtgtc cagccagccg
     4441 gtacgacgac gacgacccgt agttgcaccg aattcgttac cctgcttgca gaggaactcg
     4501 ccagtttcat caaacagttc ggccgggaaa ggacctgcac ctacacgagt tgagtaagct
     4561 ttgaggatac ccagaacgta atcaacataa cgcgggccca ggccggaacc ggtcgccacg
     4621 ccaccagcag tggtgttgga agaagttacg tacggataag taccgtggtc gatatccagc
     4681 agcgtaccct gcgcaccttc gaacatgacg aaatcgccac gctgacgcgc ctggtcgagc
     4741 aggtcagaaa cgtcaaccac catagaagtc aggatgtcgg caacagccat cgtatcatcc
     4801 agaactttct ggtaatcaac cgcttcagct ttgtagtagt taaccaactg gaagttgtga
     4861 tattccatca cttctttcag tttttcagcg aaggtttctt tgtcgaaaag gtcgccaaca
     4921 cgcagaccgc gacgtgccac tttatcttca taagcaggcc cgataccacg accggtggtg
     4981 ccgatcgctt tcgcgccacg cgctttctca cgcgcgttat ccagagcaac gtgataatca
     5041 aggatcagcg gacatgcttc agacagcagc agacgctcac gaacggggat gccacggtct
     5101 tccagttctt tcatctcttt catcagcgcg gccggagaca gcacaacacc gttaccgatg
     5161 atgctggtta cattctcgcg gagaatacct gatggaataa gatggagaac ggttttttca
     5221 ccgttgatta cgagagtatg gcctgcgttg tgaccgccct ggtagcgtac aacatattta
     5281 gcccgttcag tcagaagatc gacgatctta cctttacctt cgtcacccca ttgggtgccc
     5341 agtacgacga cgttgttacc catttttcaa aatcaccgtt tgcttaaaaa tggattctac
     5401 catcgctttt tcagagttac agcacttttt gcactcaaaa atgacctcga tcagtctgtt
     5461 ttttgttcag ccaatcgttt tcctcaacat gtagtagacc acaacgcccg caaccacaag
     5521 tccaccgcca aaacgacgta aaatattatc gggcaaattg gtcatcgcag agatcatctt
     5581 cttccatgcc ttcgggtaaa gcatcggccc taaaccttcc agtaccaaaa ccagggcaag
     5641 cgccagccag attgtcgaat tcattcatta tccttataga aaaagaaaac caccgacatc
     5701 cctgaggatg cggtggcttt attgacctgt accgcagtcg ttatattaac gcgttgcgga
     5761 agtcggcgtc ttcatgtagc ggaagaaatc gctatccggg ctcatgacca tcacgtcctg
     5821 attgccagag aagctgttct cataagcacg caggctacgg atgaatgcgt agaagtccgg
     5881 atctttactg aatgcatcag caaacagttt ggctgcttcg gcatcgcctt caccacgcat
     5941 aatgcggccc tgacgctcag cttctgccag cgttctggtc acttcatagt cggcagtcgc
     6001 gcgcagtttt tccgcttctt cctgaccttg tgaacggtga cgacgcgcta ccgcttcacg
     6061 ctcggcgcgc atacggttgt agatcgcttc agacacttcg gtcggcaggt tgatctgctt
     6121 gatacgcaca tcgacaactt caatacccag cgccgccata ctgttcgggt tgatgaccgg
     6181 aactttgccc ttcgtctcag ccgttacgcg ctctgccgct tcggcaatgg cgttatctgc
     6241 tgccggggta gtaacttcat cttctgtacc cgcagaaccg gagttcagcg cgtcacgtac
     6301 ttcgagggtc agacgaccac gggaatcggt gacgatatct ttcacgtcca ggcgaccaat
     6361 ttcagaacgc agacggtcag agaacttacg tttcaacagc acttccgctt gcgaaatgtc
     6421 gccaccaccc gttgccaggt agtaacggct gaaatcgctg atgcgccatt tgatgtaaga
     6481 gtcgacgatc aggtctttct tctctttggt cacaaagcgg tcggcctggt tgtccatggt
     6541 ctgaatacgt gcgtcgagca ttttcaccgt ttcaatgaac ggtatcttga aatgcagacc
     6601 aggctcataa accaaaggtt tgttgtcatc gtcacgcagt accttaccaa aacgcagcgt
     6661 aataccgcgc tcaccttctt tgacgacaaa gacagacatg taaagcacta ccagcacgat
     6721 gatgataatc gcgataactg acttacgcat cgttattccc cctgacgctg gtagtcgtta
     6781 cgctgcgcgt tggcgcggcg ttggtccata atatcgccct gactggtgga cgacgtgttg
     6841 cttgctccac tggttgtgga ggaagaggct ggcggcagac gcagcagatt gctggcaccg
     6901 ttatcgctct tcgccgcagg ggcgttacca cctttcagca tctggtctaa cggcagaacc
     6961 atcaggttgc cacctttatc gttaaccagc actttgcggg tgttacccaa cactttttcc
     7021 atcgtctcga tatacagacg ctcgcgagta atttccggcg cggctttata ttccggcaga
     7081 agtttagcaa agcgcgccac ttcaccctga gcttccagga tggtctgggc cttgtacgca
     7141 cgcgcctctt cgaggatacg ttgcgcctga ccgttcgcac gcggctgaac ttcgttggta
     7201 tacgcttctg cttcacgaat gtattgctgt tcgttttcac gcgcggcaat cgcatcgtca
     7261 aacgccgctt ttacttcttc cggcggacga gcagcctgga agttgacgtc cagcagcgtg
     7321 atacccatgt catacggacg aatcgtctct tccagttcgc gctgagtatc gctacgaatc
     7381 acggtacgac cttccgtcag aatgcggtcc atggtgtatt taccgataac tccacgcagg
     7441 gcgctgtcgg tagcctgacg caggctgtca tccgggctgg tcacgctata cagatatttt
     7501 tccggattgg tgacgcggta ctgcacgttc atctcaacgc gcactacgtt ctcgtccgac
     7561 gtcagcatca caccagaagc ggccagttca cgcacggctt ccacgttcac cggtttgact
     7621 tcgtcgataa acgtcggttt ccagttcaga cccggctcaa ccagatggct gaatttacca
     7681 aagcgtgtta ccacgccgcg ttcggcttct ttaatggtat agaaaccact ggccgcccag
     7741 ataatgacaa tcgctgccgc tgcgatggta acgacacgac cgccaagctg cgggcgcggg
     7801 ccttgcgatg aactgccacc gccagatccg gtgcctttac cgccgcccag accaccgagc
     7861 tttttgctca gtttgcggaa gatatcatct aaatcaggtg gcccttgatc gcgaccgcct
     7921 ttgtttccat ttccctcaga gttgccgcca ggtttgctgc ttccccacgg gtcgcggtct
     7981 tgtccgttat taccgggctg attccacgcc atgtttgtgc tccatatttg ttatgcggtg
     8041 atccctgttg tctttcgacg ccagaggata tgactccacg cttcagacgc tacgccgtta
     8101 gatcaggtaa tcgatcaacg ccggttcttg tttacagagg cgacgccagt caacgatcgg
     8161 catacgaact tgcagactta cgctgccgtc ctcctccatc cactcttttt ctattgcctg
     8221 aagctgataa aaacgactcc tcagacgccc ttcctgcggt ggcagacgca atgtatgctg
     8281 cgccacctcg ccggaaagcc gctccgtcaa agcctgaaaa agctgtggta tccccgctcc
     8341 ggtctgtgcg gaaagccaga cacggatcgg tttgttctct tcgtcccgat caatacgcgg
     8401 ttcgaaatct tccagcatat cgatcttgtt catcaccagc agggttggga tctcgtgagc
     8461 gtcgatctct tcaagaaccg tattcaccgc ttcgatgttt tcttgtacac gcacatccgc
     8521 cgcatcaatg acgtgcagca gtaatgtggc ttgccgcgtc tcttgtaacg tggctttaaa
     8581 tgccgccacc agatcgtgcg gcaggtggcg aataaaccct acggtatctg caagtacggt
     8641 ttcaccgaca tctgcaacgt caatacgccg caacgtcggg tcgagggtgg caaacaactg
     8701 gtctgccgcg tagacccgcg cttcggtgat gcgattgaaa agggtagatt taccggcgtt
     8761 ggtatatccc accagcgaaa cagtaggaac gtcggctttg atacgcgatt gccgcccctg
     8821 ctcacgctgc ttttcaactc tttccaggcg cgactgtatc tgcacgatgc gattacgcaa
     8881 caaacgacgg tcggtttcga gctgggtttc acccggacca cgcaaaccta tcccgccttt
     8941 ctgtctttca aggtgggtcc agccacgcac caggcgcgta gccagatggc gcagctgcgc
     9001 cagctcaacc tgcaacttac cctcatgggt acacgcacgt tgggcgaaaa tatctaaaat
     9061 aaggccggtg cggtcgataa cacgacactc gcacaaacgc tccaggttac gctcttgcgc
     9121 cgggctcagg gcatggtcaa aaagaacgac cgaagcaccc atcgctttga cagcttccgc
     9181 aatttcaact gctttacctt cacctacaaa atactttggg tgcggcgctt tacggctacc
     9241 ggtaatcacc tgcaatgctt cgacaccggc ggaagagacc agagattcaa actcctggag
     9301 gtcttccata tctttgtctt gcgtaaaata gatgtgtacc agtaccgcct gctcaccagc
     9361 atcataacgg tcaaacaagc gtataaccct ctaaatagat cagcggggaa cgcaggatcg
     9421 ctggctcccc gtgtaaaaaa acagcccgaa accttattcg gtttcttcgc tgtcctgttg
     9481 cgcggaagta ttctgcgcgc tgctaccatg atggtagtta ctgctggtac cgccaccggc
     9541 gttgttactg tgatgagaaa ccgggcgaga tgggacaaca gtagaaatcg cgtgcttgta
     9601 aaccatctgg ctgaccgtgt ttttcaacag gatcacgaac tgatcaaaag actcgatttg
     9661 cccttgcagc ttaataccat tcaccaaata aatagaaact ggaacacgtt cccgacgcag
     9721 tgcgttcagg aacggatctt gtaaagattg ccccttagcc attctctctt ttccttatat
     9781 gcttatttgt actttgaacc tttcgattct gaaaaaattg cgcacgatac gtctcaattg
     9841 tacacattca gcctgcgata gcaccaacaa cctgtaatac ttcgtcacgc gcctgttctg
     9901 gtttttcact gtcaagccag tgaacccctt cccaaccacg cagccaggtt atctgccgct
     9961 tcgccaactg tctcgtggcg caaacacctc gataaaccat ttcatcgtat gagatttcgc
    10021 cttcaaggta agaccacatc tggcgataac ccacgcaacg aatggaaggc aagtccgtat
    10081 gcaaatctcc tcgggcaaaa agcgcccgga cttctgcttc aaaacctgaa gccaacatct
    10141 gatgaaaacg ctgctcaatg cgttgatgga gcagttcacg gctcgccggg gcgatggcga
    10201 actgatgcac ctgatacggt agagcgtctc ctgacgtttg cgtcagttcc gttaaagttt
    10261 tacccgaaat gaaaaaaact tccagtgccc gggaaagcct ttgtggatca tttggatgaa
    10321 tccttgctgc cgcaaccgga tctacctcct gaagttgacg atgcaatgac tcccaacctt
    10381 gctctgccgc ctgttgctca attctggccc gcacttccgg gtctgccgac ggtagcggcg
    10441 acaacccttc cagcaatgcc ttgaaataca acatcgtacc gcccactaac agtgggatcc
    10501 gccccgccgc ggtgatatcg gccatttccg ccagcgcatc gcggcgaaaa tcagcagccg
    10561 agtaagcctg cgacggatcg cgaatatcca gcaatcggtg cggcgcggcg agtaactctt
    10621 cagcgttcgg cttcgccgtc ccgataacca tccctttgta aataagggca gaatcaacgc
    10681 ttatcaactc tactggtaaa attttacgca gctcaatggc taacgccgtt ttaccggagg
    10741 ccgtcggccc catcaaaaaa atcgccttag gcaggctcgc cttactgata tcactcatct
    10801 ttcagggctt ttatcgccgg atgtaaatca acagattgta acagaccacc cggcggcgtt
    10861 ttcacaagtt gcggacataa ccgttccacg tccgccagca gggttatggc ctgtgccatt
    10921 gaccactgcg catgttcgct catcagattt cgtgcaatcc actgcgcaat attgccaggt
    10981 tcgaatacgg actgcttcgc caggtagcct atcagttcag gaatcaagat ttgtaaattt
    11041 tgttggcgta agggtaaagg cacggccctg atggtcacat gctgtgcatc tgactggaaa
    11101 tcaataccca attccgctag ggcagactgc gctttttcta atgccgattt ttcttcgcca
    11161 gaaactttta gccgcaacgg aatcagcagc ggctgggcgc aaacgggcgc ttcacccggc
    11221 gtcaattgtg cctgacgcag ccaacgttct gccactggca aggataaaag tgaaatgttg
    11281 ccatcgcgct ccagcaacgc acagtcggaa tggacgatag tcagtacccg accaaaactc
    11341 tgactgttcg ccgcaagtgc aggttcctgc ggttccggcg cttttggttt ttgcatcggc
    11401 gcgggcgttt gcaaaagctg gcgatacact tcaccttgct gtttctggta gcctggctgc
    11461 gcattcggcc agggggcagc cggacgactg cctgaggcgg gcgcaggaga gtagcgcgga
    11521 gctaccggct cacgagctgc cggttctgca aagtgattgc gccccgccgc cacgcggttt
    11581 tccgggatgg cacgcggtgc gggttgtggt tcatcgtcca gcggtagcgg cgtttccagt
    11641 tgctgttgta gcacgctcag cacgccctga tagataaaat catgcaccag acgcgactga
    11701 tggaaacgca cttcgtgttt ggcggggtgt acgttgacgt ccacctgatg cgggtcgatc
    11761 tccagataca acacaaatgc cggttgctga tcggccccca gtttgtcttc gcaggcctgg
    11821 cggatcgcat gattgatcag gcgatcgcgc atcatgcgac cattcacgta gcaatactga
    11881 atttccgcca gtgcgggcgt agtgtgattt ggatcggcca cccagccgcg cagggtgaga
    11941 tcgccgtgtt gccattcaat cgccagcgct tgttcgagaa aagcggtgcc gcaaatcgcg
    12001 cctaagcgcc gttctttttg cccgccttcc ggcactgcgc ggtactgacg cacaatttta
    12061 ccgttatgcg acaggttgat cgtgacgtcg aaacgcgcca gcgcaatgcg gcggatgatt
    12121 tcatcaatat ggctaaattc ggttttctcg gtgcgcagga atttgcgccg cgccggggtg
    12181 ttgtagaaca gatccagcac ctccagcgtc gttcccaccg gatgcgctgc cggtttaacc
    12241 gtcacgtcca tgtcgcgtcc ttcggcatag gcctgccagg cttcctgctg ttctgcggtg
    12301 cgtgaagtga gcgtcaggcg ggaaaccgaa ctgatactcg ccagcgcctc accgcgaaag
    12361 cccaggctga taatggcttc gagatcgtcc agagaggcga ttttactggt ggcatgacga
    12421 gccagcgcca gcgccagctc atcttttttg ataccgcagc cgttatcacg aatgcggata
    12481 agtttcgccc caccgcgttc gatatcaata tcgatacgcg tcgcacctgc atcgaggctg
    12541 ttttccacta gttctttgac taccgacgca ggtcgctcga ccacctcacc tgcggcaatc
    12601 tggttcgcca gttgtggcgg taagacctga attggcatca atcgtcctta gtttggcagc
    12661 gtgcgatctg gcgtcgtcac cgtactggca gtttgtgccg ttgcaccctg cggcgcagat
    12721 tgcatcggat gcgcaaggaa gtaattgcgc aggcctttgt aaatggcttc tgccagctgt
    12781 tgttggtaat cgtcgctcgc cagcaaacgt tcttcgctgt tgttgctgat aaaaccggtt
    12841 tcgaccagta ctgatgggat atccggcgag cgcagaacgc caaggctggc gtgttctggt
    12901 cgacgtttat gtatttcgcc aatgcgttgc aactgactga tcatactggt cgctacatca
    12961 taccctaccc gctgggaatg accgaactgt aaatccagca ccgcctggct taaatagggg
    13021 tcagactgac tgttcgccag cacatcaccc gccccaccca gcagctccga cagtttctcg
    13081 tactgctcca gccagctggc catttcactg ttggcgcgac ggttagagag cacccatacg
    13141 gaagcgccag tcgcactacg gttcggtgcg gcatcagcgt gaatcgacac gaggaaattg
    13201 gcgttttgct tatgtgccac atcactgcgc cccatcaccg agataaagta atccccgtca
    13261 cgggttaaaa cgcctttaaa catcggatcg tcattgagca aagtacgcaa tttacgcgcg
    13321 atggcgatgg tgacattttt ctcccgcgta ccaccggggc cgatagcgcc agggtcctga
    13381 ccgccgtgtc cggcatcaat agcgatgata attttatcgc cagtgttagc cgtcgcgcgc
    13441 gctgccggac gcgttaccgt attactgctg ataacacccg tagtgcggtt actttccgtt
    13501 ttaaacggat tgcgcgccgg ttcgctgacg cgcggtgcga caaccgcagg cgtttcaacg
    13561 cgtttcgcaa ccacgggcgg cggaggaggc ggtggcggca catcggcgtt aatcgtaaag
    13621 acgacagtgt aattgctgcc attctgccgc ttcaccgctt cggttttacc gttttcggta
    13681 agatcgacca ccagccgtag cgtttgtgca tctttaggcg ttccagagcg aatcgccttc
    13741 accagattat tgccgctgaa caacaacggc agtccctgaa tcacgcccgt ttgtttgata
    13801 tcgagcgcca cggtgcgttt gctttgatgg ctaaacgcat aatcaggatc gccaataaaa
    13861 ctcaacgtta tccgcgcctg ttgattaccg ttagaaacct gaatatcaga gagcgtcgcg
    13921 gcacccaccg gcgtgcacag cagcagcagc gtcgctacca accaatttct gatgcgatac
    13981 atcatcccgc cacctttcaa aggttaaccg gctaaacgcg ccagcaacaa ttcacccgca
    14041 gaggaaaccg cactcacgcg cgcctcacgg ccttgtgcct gataatcaat gtgtatttcg
    14101 acatccgggt caggaagaac acctgtacct tgttgtggcc actccaccag gcagatggca
    14161 tcgttggcaa aataatcgcg gatccccata aactccagct cctcgggatc ggcaaggcgg
    14221 tacaaatcaa agtgatagac cattaagttg tcgagcgtat agggttcgac cagcgtataa
    14281 gtggggcttt tgacattacc ctgatgaccc agagcctgta aaaagccccg gctaaaggtg
    14341 gttttacctg cgcctaaatc gccatacaga tagattacgg ttgcgccatc gcaggctttc
    14401 gctacccgct cgcccaggtc taatgttgcc tgctcatcag ggagcggaat tactcgattc
    14461 atcatggttt ttatcagtca cttccgggtt aacaatacgc tgtagcgtgg aaaagagatc
    14521 ggttgccagc atcccgcgcg ttccaaaacg cgccgccagt acgtcagctg ccgcaccgtg
    14581 cgcgacacag cctgcacagg ctgcatcata cggcgacagt ttttgcccaa gcaatgcgcc
    14641 aataatacca gagagcacat cgcccatgcc gccgctcgcc atgcctgcat ttccggcatc
    14701 aataatgcct aaagcgtcag gatgggcggc gaccacggtt ccggcacctt tcagcaccgc
    14761 tacgccgcca taacgttgta ccagacgttt ggcgcaatgt aagcggtcac tttcaatttc
    14821 agcgacggaa cagcctaaca accgtgcggc ctcgctagga tgcggcgtga tcacgcgatt
    14881 gtgacgctta tcgggattga ttgccagcag gttcaatgca tcggcatccc acaacatcgg
    14941 tttgcgaaaa ttctcaactt tttgcagggc ttttttcccc cactcttgct ggcccagacc
    15001 gggaccaatc accaccacat cggcccattc caggctttcg gtaagagagt ccatagtcag
    15061 ttcatgcacc atcaattctg gtcgtgcagt cagcagcggc gcaatgttct cactgcgggt
    15121 cagtactcgg actaaaccag caccagcacg tagcgccgct tcccccgtca tacgaatagc
    15181 ccccgccgtg ccgtgatcgc caccgataat caccagccgc ccgtgatcgc ctttatgcga
    15241 agtcgggcga cgcggtttca gccagtgaga aagttgttct gccgaaaacc gctgaatttt
    15301 cgtctcctga cctgccagcc agctatccag ccccagtgag tcaaaatgca gttgtcctgt
    15361 aacatcccgc gcttttccag tgagcaagcc tagtttcagc gcaataaaag tgatggtgtg
    15421 atcggcgttg atcactgcgc ctggcgtagc gccggtttca gccagcaggc cggaagggat
    15481 atcaaccgcc acaatcggcg caggatggga attagcgtgg tcgattaact ggctaatgga
    15541 ttcgcggggc gcttgctgca agccggtacc gagcagcgca tcaacaatca gatctaccga
    15601 ttcgggccag acaatattcg aagcatggat ctcgccaccc gcgtttaacc atgcttcgcg
    15661 tgccagcgcg gcctcttccg gcaacggttt gtcgctctcc tgggccaaca acgtgacctc
    15721 aatgccgacc gctttggcca gtcgcgcgac cacgtagcca tcgccgccgt tattaccatg
    15781 accgcacagc accagccagt ggcgggcgtc aggatacgcc gaacgacaca cctggaatgc
    15841 ggcctcgcca gcgcgaagca tcagctcata gagtgtgagc cccagcacat ctgccgcctc
    15901 gcgttctccg cggcggatat cgtcggcgta ccagacggtg tgtggtatac ttacggggtt
    15961 tttcttcatt gtatggtccg tcatgtcaga gcccctcgat ctcaatcagt tagcgcaaaa
    16021 aattaaacag tgggggctgg aactgggctt tcagcaggta ggtattaccg ataccgatct
    16081 cagcgagtcc gagcccaaac tgcaagcatg gctggacaaa caataccacg gcgaaatgga
    16141 ctggatggca cgtcacggta tgctgcgcgc tcgcccccat gagttattac ccggtacgct
    16201 gcgtgtaatc agcgtgcgga tgaattacct tcctgctaac gccgcatttg ccagcacgct
    16261 gaaaaacccc aaactcggct atgttagccg ttatgcgctg ggccgtgact atcacaaact
    16321 tctgcgcaac cgactcaaaa agctgggcga gatgattcag caacattgtg tttcgctgaa
    16381 ttttagaccg tttgtcgatt ctgcgcctat tctcgagcgc ccgttagctg aaaaagctgg
    16441 gctcggctgg acaggtaagc actcacttat cctcaatcgc gaggccggtt cgttcttctt
    16501 tttaggcgaa ttgctggtcg atattccgct gcccgtggat caaccagtcg aggaaggatg
    16561 cggcaaatgc gtggcctgta tgacgatttg cccgaccggt gccatcgtcg agccatatac
    16621 cgtcgatgct cgccgctgta tctcttatct caccatcgaa cttgaagggg cgctcccgga
    16681 agagttgcga ccgttaatgg gaaaccgtat ttacggttgc gatgactgcc agcttatctg
    16741 cccgtggaat cgctattcgc aactcactac agaagacgat ttcagcccgc gtaagccgct
    16801 acacgcaccg gaactcattg agttatttgc ctggagcgaa gagaagtttt taaaagtcac
    16861 ggaaggatcg gcgattcgcc gtataggtca cctgcgctgg ctgcgtaata tcgccgtggc
    16921 attaggcaat gccccttggg atgaaacgat tttgacagcg ctggaaagtc gtaaaggtga
    16981 gcacccactt ctcgatgagc acatagcgtg ggcgattgcg cagcaaatag agagacgaaa
    17041 tgcgtgcata gtcgaagtgc aattgccgaa aaaacagcgt ctggttcggg tgattgaaaa
    17101 agggttaccg cgtgacgcct gattcatcca cagcttgtga ataaaaataa aaacgcattg
    17161 caattcaaga cgaataaaat cgtcaagtga tcgactcgac aaaacggaat cttgattttt
    17221 aacttattta tcaataggtt aaaaaatacc attcaccgtg cgaagtttct ttgaaattaa
    17281 gattacagcc tgaggctgtg gataactctg tttacaagag tttgtcagag acaacaaaaa
    17341 ccagcaataa cgcatcgcgt cgctgtagat attttattga gagaagaatt tggagc
//
LOCUS       DAJTQY010000079        17223 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.079, whole genome shotgun sequence.
ACCESSION   DAJTQY010000079 DAJTQY010000000
VERSION     DAJTQY010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17223
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.079"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(71..862)
                     /gene="mazG"
                     /locus_tag="OO349_002853"
     CDS             complement(71..862)
                     /gene="mazG"
                     /locus_tag="OO349_002853"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HCR5867854.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     VNEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(958..3192)
                     /locus_tag="OO349_002854"
     CDS             complement(958..3192)
                     /locus_tag="OO349_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HCR5867855.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(3240..4541)
                     /gene="rlmD"
                     /locus_tag="OO349_002855"
     CDS             complement(3240..4541)
                     /gene="rlmD"
                     /locus_tag="OO349_002855"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCR5867856.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVAVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHDVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILAPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            4598..7354
                     /gene="barA"
                     /locus_tag="OO349_002856"
     CDS             4598..7354
                     /gene="barA"
                     /locus_tag="OO349_002856"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HCR5867857.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISCVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSIQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQR
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLAGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(7586..8926)
                     /gene="gudD"
                     /locus_tag="OO349_002857"
     CDS             complement(7586..8926)
                     /gene="gudD"
                     /locus_tag="OO349_002857"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCR5867858.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDASGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(8947..10287)
                     /locus_tag="OO349_002858"
     CDS             complement(8947..10287)
                     /locus_tag="OO349_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCR5867859.1"
                     /translation="MATQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKSFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAIFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPCGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(10289..11641)
                     /gene="gudP"
                     /locus_tag="OO349_002859"
     CDS             complement(10289..11641)
                     /gene="gudP"
                     /locus_tag="OO349_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HCR5867860.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPVQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQSTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(12075..12524)
                     /locus_tag="OO349_002860"
     CDS             complement(12075..12524)
                     /locus_tag="OO349_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HCR5867861.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(12542..13324)
                     /gene="truC"
                     /locus_tag="OO349_002861"
     CDS             complement(12542..13324)
                     /gene="truC"
                     /locus_tag="OO349_002861"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HCR5867862.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRYLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(13324..13653)
                     /locus_tag="OO349_002862"
     CDS             complement(13324..13653)
                     /locus_tag="OO349_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HCR5867863.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWGLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(14275..14820)
                     /gene="syd"
                     /locus_tag="OO349_002863"
     CDS             complement(14275..14820)
                     /gene="syd"
                     /locus_tag="OO349_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HCR5867864.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            14888..15736
                     /gene="queF"
                     /locus_tag="OO349_002864"
     CDS             14888..15736
                     /gene="queF"
                     /locus_tag="OO349_002864"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HCR5867865.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            15848..17212
                     /gene="ppnN"
                     /locus_tag="OO349_002865"
                     /gene_synonym="ygdH"
     CDS             15848..17212
                     /gene="ppnN"
                     /locus_tag="OO349_002865"
                     /gene_synonym="ygdH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HCR5867866.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
BASE COUNT         4216 a         4659 c         4134 g         4214 t
ORIGIN      
        1 tgtttttgac gcaaatcaag cgcttgtcat ttaaaaaatg acacaaatgg cgcttgaccg
       61 cgtaattccc ttagagatca atttcctgcc gttttacctg ttgccagact tcttccattg
      121 tttcgaggtc aacacctgtc atttccagtc cacgcgcggc aacaatacgc tccacttcgc
      181 gaaaacgacg ctcgaatttt tcgttcactt tttgcaatgc gatttctgct ttcgtcccta
      241 aatggcgagc cagattaacc gtggcaaaca gcaggtcccc catttcctcc tccagtttag
      301 cctggtcgac aacagcctgc cgcgcctcgt acatcacctc gtcgatctct tcgtagactt
      361 tatcaaccac cgggccaagc gtcgtccagt cgaagccaac gttggcgcaa cgtttctgga
      421 ttttttgcgc ccgcattaaa gccggtaaac tacgaggaat atcgtccagc gccgaatgct
      481 gcgctttctg cgcgcgctct tcggttttga tttgctccca acgggcaagc acttcactac
      541 tgttttcggc agaactatca gcaaaaacat gcggatggcg acgctctaat ttatcgctaa
      601 tagcagcgca aatatcatta aagtcaaagc gcccttcttc ctgagccatt tgcgcgtaaa
      661 acaccacctg gaatagcaga tcgcccagtt caccgcgaag atcgtcaaaa tcttcacggg
      721 cgatggcgtc cagcacttcg taggtttctt caagggtgta aggcgcaatg gtggcaaatg
      781 tctgctcttt atcccacggg cagccgtttt ccggatcgcg caggcgctgc ataatagtga
      841 gcaaacggtc gatttgattc attgaattgt cctgaaaatt gcgggtctgt caggtgtaat
      901 accttcgagt aaaagatatc gctggatacg gctgacgcca tatccagcta tgtaagacta
      961 actcccgtgc aaccgacgcg cgtcgataac atccggcacc tggttgagtt tacccagcac
     1021 gcgccccagc acttgcaggt tgtaaatctc aatggtcatg tcgatggtcg ccagttgctg
     1081 tttggtgtcg ctacggctgg caacgccaag cacgttcacc ttctcgttgg cgagaatggt
     1141 cgtgatatca cgtaacaacc cactacgatc attagccacc acgcggacca ccagcgaata
     1201 tccggcggag tagctctcac cccataccgc gtcaacaatg cgttctggcg catgggagcg
     1261 cagttccgcc agttgttcgc aatcggcgcg gtgtactgaa ataccgcgtc cctgggtaat
     1321 gaagccgaca atctcatctc caggaatcgg ctggcagcag cgcgcgatgt ggtgcatcag
     1381 gttaccaaca ccttcaacca ccacacgacc gttatcttta ctgcggtttt gcggtgtgta
     1441 gcttttttgc tgaagctgtt tcagcgcggc ggcgtcctgc tcttcggcac tcggcttatt
     1501 aaattgcgat tgcaggaagt tcaccatctg attgagacgg atatccccgc caccaatcgc
     1561 cgccagcaac tcgtcgacat cattgaagtt gtaacgcggc agcagatgtt tttctgcttc
     1621 tttcaggctg atccccagat gttccagctc gtcgtcaagg atttgccgcc cagccagaat
     1681 gtttttgtca cggtcctgtt tacggaacca ggcgtgaatt ttcgaacgcc cacggctggt
     1741 tgtgacgtaa ccgaggtttg ggtttaacca gtcacggctg gggttcggct gtttctgggt
     1801 gataatttca atctggtcgc ccatttgcag ctggtaggtg aacggcacaa tgcgcccgcc
     1861 aatttttgcc ccgatgcagc ggtgtccgac atcactgtgg atgtggtaag cgaagtccag
     1921 cggcgttgat cccgcaggca aatcaacgac atcacctttc ggcgtaaaga catacacccg
     1981 gtcgtcaaac acctgactac gtacttcgtc gagcatttcg ccggaatcag ccatctcttc
     2041 ctgccacgca atcagtttac gcagccaggc aatccggtct tcatgtcccg aacgtgcgcc
     2101 gccagctgcc gcgccctctt tatatttcca gtgcgcagca acacccaact ccgcatcttc
     2161 atgcatctgt ttggtgcgga tttggatctc aacggttttt ccacccggcc ccagaaccac
     2221 ggtatgaata gactgataac cgtttggttt cgggttagcg acgtaatcgt caaactcatc
     2281 cggcaggtgg cgatagtgag tgtgcactat ccccagtgcg gcataacaat cctgtaaacg
     2341 ctcggcgaca atacgtaccg cacgcacatc aaacagctca tcgaaggcga ggttcttttt
     2401 ctgcattttg cgccagatgc tgtagatgtg tttcggacga ccatacactt cagctttaac
     2461 gccttcagct ttcatctcag cgcgcagatg gccgacaaac tcttcgatat agtgttcgcg
     2521 gtcgagacgg cgttcatgca gcagttttgc gatgcgtttg tattcggtcg ggtggagata
     2581 acggaagcag taatcttcca gctcccattt cagttgccca atcccaagac ggttcgccaa
     2641 cggcgcgtag atattggtgc actcttttgc cgccagtaca cgttcatctt ccggcgcatc
     2701 ttttacttcg cgcagatgag caatacgctc cgccagtttg atgactacgc agcgaaaatc
     2761 atcgaccatc gccaataaca tccggcgaac gttatcgacc tgttcggagg aaacagaatc
     2821 agtgtgcgtc gctttcagct ggcggatcgc cgccatatca cgcacgccgt gaataaggtt
     2881 aacaaccgac ttaccgacgc tctcacgcag cacatcttcg ctgactacgt tggcatcagc
     2941 cagagggaac agcagcgccg cccgcagcgt gtcaatgtcc atacttaatg tcgagaggat
     3001 ctccaccatc tcaacaccac gccacaataa cagactggca tccggatgcc cctgcgtctg
     3061 ttgcagacaa tacgcccagg tttcggctaa gcactcacac gacttctggc tggtaatacc
     3121 cagacttgcg atccattttt ccggatcaaa ttcaccagcc ttattgatat gtgcacttct
     3181 taccgcaacc atcgtcctct cctttaggga ccagacctgc cgaaatcggc aaatcgtaac
     3241 tatttaacgc gcgagaaaag taccatcgat tccagatgtc ccgtgtgtgg gaacatatcc
     3301 agcatcgcca gtcgcgcaat ggtatatcct gcttttaata acgcttcgct atcccgagcc
     3361 agcgttgcag ggttacagga tacataaact atacgaatag gttccagttt tataatttgc
     3421 tgcataacac ctgcggcacc tgctcgcgcc gggtccagca acactttatc gaagccgttt
     3481 ttcgcccacg gctgctttgt gacatcttct tcaagatttt cgtgataaaa cgtcacattc
     3541 tgtaagccat taagacgcgc attctgctgg cctttttcca ccagcgcagg aacaccttct
     3601 acaccgacca cactggcagc ttgtgtcgcc aatggcagtg taaagttgcc cataccgcag
     3661 aacagatcca gtacgcgatc ttcaggttgc acatccagcc attccaacgc acgcgctacc
     3721 attttttggt tcacacccgc attgacctga ataaaatcgc gcgggctaaa agttaagcgc
     3781 aacccgtttg agtcatacca gggcatctca ccagagacgg tttcgagtat ctcactatcg
     3841 ggggcgagat acagatccag gccttcagaa tgcgaaaagc gttccagttt ttcgcgatct
     3901 gccgaactta gcggtgcggt atggcgcaaa atcatcagcg tgccgctggt tgcctgtacc
     3961 agttcaacat gaccaagatg gcgcatagct tgtaagctgc ccagacatgc cctgactttg
     4021 ggcagcaatg cttcaagttg gggcgctaaa atggggcatt gtttgacgtc gacaatgtca
     4081 ctggagcccg ctttgcgaaa ccccatctga agttgctgtg ttttcggtaa gtagttcaga
     4141 cttaaacgcg cgcggcggcg atagccccag ggaacatcgg cgatcacttc agagacatcg
     4201 tgtttcatta atcgggcgag tgccgcactt ttgcttcgct gctgtaaatc cacgctggcg
     4261 tgttgttgct gacagccgcc gcatacgcca aaatgaggac agcgtggcgt ttcgcgttcc
     4321 gggctatcgc ttaaccggcg tacgacttta gcgcgggcat actgtttttt atcttcagta
     4381 acagcaactt ccgcgttttc ctgcggcaat aatccgggga taaataacgt tttgccgtta
     4441 tgtcgcgcca cgccctgacc aaaagagtcg aggtcgttga ctgaaacggt tatgatctga
     4501 cgcgtcgtcg tgcgtcgttt tgcagagtag aattgcgcca ttggcgagac tttctcaatt
     4561 taacagtgtg accttaattg tcccataacg gaactccatg accaactaca gcctgcgcgc
     4621 acgcatgatg attctgatcc tggcaccgac cgtccttatt ggtttattgc tgagtatctt
     4681 tttcgtcgtg catcgctata acgacttgca gcgtcaactg gaagatgccg gtgctagcat
     4741 tattgagccg cttgcagttt ctactgaata tggcatgagc ctgcaaaatc gcgaatctat
     4801 cggtcagtta ataagcgtac tgcatcgtcg ccattccgat attgttcgcg cgatttcggt
     4861 ttatgatgaa aataaccgac tctttgtcac ctccaatttt catcttgatc cctcatcaat
     4921 gcagctcggc agcaacgtgc cgtttcctcg ccagctcact gtcactcgtg acggcgatat
     4981 tatgatcctc cgcacgccga ttatttctga gagttactcc cccgacgaat cgcccagtag
     5041 cgatgccaaa aatagtcaaa atatgttggg atatattgcg cttgagctgg atcttaaatc
     5101 ggttcgcttg cagcaatata aagagatctt tatttcctgc gtgatgatgc tgttttgtat
     5161 cggtattgcg cttatttttg gctggcgctt aatgcgcgat gtaaccggtc cgattcgcaa
     5221 catggtgaat accgtcgacc gcatccgtcg cgggcaactc gacagccggg tggaaggatt
     5281 tatgctcggc gagctggata tgctgaaaaa cggtatcaac tcgatggcaa tgtcgctggc
     5341 tgcttatcac gaagagatgc agcacaatat cgaccaggcg acgtccgatc tgcgtgaaac
     5401 gctggagcag atggaaattc agaacgttga gttggatctg gcgaaaaagc gcgcccagga
     5461 agcggcgcgt attaaatccg agtttctggc aaatatgtca cacgagctgc gtacaccact
     5521 gaatggtgtt attggcttta cccgcctgac gctgaaaaca gaattaacac caacgcagcg
     5581 cgatcacctg aatacgattg aacgttcggc aaataatttg ctggcaatta ttaatgatgt
     5641 tctcgacttc tcgaaactgg aagcaggtaa gctgattctg gaaagtattc cattcccact
     5701 acgcagcacg ctggatgaag tcgttactct gctggcacat tcttctcacg ataaagggtt
     5761 ggaactgacg ctcaatatta aaagcgacgt gcctgataac gtgatcggcg acccactgcg
     5821 attacagcaa atcatcacta acctggtggg gaatgcaatt aaattcaccg agaatggcaa
     5881 cattgatatt ctggtagaaa aacgtgcgct gagtaatacc aaagtgcaga ttgaagtgca
     5941 gattcgggat accggcattg gtattcctga acgcgatcaa tcgcgcttat tccaggcctt
     6001 ccgacaggct gatgccagta tttcccgccg tcatggtggc accgggctgg ggctggtgat
     6061 tacacaaaaa ctggttaatg aaatgggcgg cgatatttcg ttccatagcc agccgaatcg
     6121 cggttcaact ttctggttcc acattaatct cgatctgaac ccgaacatta ttatcgaagg
     6181 gccatccatc cagtgcctcg caggtaagcg cctggcctat gtcgaaccaa actccgcagc
     6241 agcgcaatgc acgctggata ttttaagtga aacgccgctg gaagtggttt atagcccaac
     6301 gttctccgcg ctgcctcccg cgcattacga catgatgttg ttaggcatcg cggtgacctt
     6361 ccgcgagccg ctaacaatgc aacatgagcg attagcgaaa gcggtatcga tgaccgattt
     6421 cctgatgctg gcacttcctt gccatgcaca agttaatgct gaaaaactca agcaagatgg
     6481 tatcggcgcg tgtctgctga aaccattaac acctacgcgc ctgttgcctg ccctgacgga
     6541 attttgtcat cacaaacaaa acacgctttt gcctgtaacc gatgaaagta agctggcaat
     6601 gacagtcatg gcggttgatg acaaccccgc taacctgaaa cttatcggcg cattgctgga
     6661 agatatggtg caacatgtgg aactttgcga tagcgggcat caggcagttg aacgggcgaa
     6721 acagatgccg ttcgatttga tcttaatgga tattcaaatg ccggacatgg atggcattcg
     6781 ggcctgcgaa ctcatccacc aactcccgca tcagcgacaa acgccggtta tcgcggtaac
     6841 ggcgcatgca atggccgggc aaaaagagaa gctgcttggc gcagggatga gcgattatct
     6901 ggcgaaaccg attgaagaag agcgattgca taatttgttg ttgcgctaca agcctggcag
     6961 cggtatttcg tctcgcgtcg tgacgcccga agtcaacgaa attgtggtga acccgaatgc
     7021 gaccctcgac tggcaactgg cactacgcca ggcagcagga aaaaccgatt tagcgcgcga
     7081 tatgctgcaa atgttactcg atttcctgcc tgaagttcgc aacaaagttg aggaacagct
     7141 ggctggagaa aacccggaag gcctggttga tttgattcat aaactgcatg gcagttgcgg
     7201 ctatagcggc gtaccacgta tgaagaatct ctgccagctg atcgaacaac agctacgtag
     7261 cggtactaaa gaagaagatt tggagccgga gctgctggaa ctgttggacg agatggataa
     7321 tgtcgcgcgc gaagccagca aaattctcgg gtaacggacg tcttgtcgag tagcaatcgg
     7381 ctttttatga cgctggtttc agattggtga caaagtgcct gtgtttatgc cggatgcggc
     7441 gttaacgcct tatccggcct acaaaaccaa ttaaattcaa tgaattaaaa aatgatgtag
     7501 acctgataca tagcacaaca ggctattttg cgtttagcat cagtctcaaa ccggctccag
     7561 atagagccgg ttttggtttt ctgtcttaac gcaccatgca cgggcgcttg ttatcgaacg
     7621 tccagccagg aatcagatac tgcattccca tcgcatcgtc acgcgcgcca agcccgtgtt
     7681 tctgatacag ctcatgggct ttcatcactt gatccatatc gatttctaca cccagccccg
     7741 gtttttctgg cacctgtacc agcccgcctt tgatctcaaa cggttctttg gtcaggcgct
     7801 gattaccttc ctgccagatc cagtgcgtat caatagcagt aattttaccc ggtgcagcgg
     7861 cggcaacatg ggtaaacatc gccagggaaa tatcgaagtg gttgtttgag tgtgaacccc
     7921 aggtcaggcc aaattcatgg cacatttgcg ccacacgtac cgaaccttgc atagtccaga
     7981 aatgcggatc cgccagcggg atatcaacgg attgtaacga gagcgtatgg cccatctgcc
     8041 gccagtcggt ggcgatcata ttggttgcag tcggcaggcc agtcgcgcga cggaactctg
     8101 ccatcacttc acgcccggag aaaccttgct ccgcaccaca cggatcttct gcataagcca
     8161 gcgaaccttt caggtattta ccaattttaa ttgcttcgtt cagcgaccag gcaccgttag
     8221 gatcgagcgt aatacgcgcc tgcgggaagc gttgcgccag tgccacaata gactcggctt
     8281 cttcttcccc ggccagtaca ccgcctttca gtttgaaatc gttgaagcca tatttttcat
     8341 acgccgcttc cgccaggcgc accaccgcat ccggcgtcat cgcttcttca tgacgcaggc
     8401 gataccagtc gcatgagtca tccggctggc tttgatacgg cagaggtgtg gctttgcgat
     8461 taccgacgaa gaacagataa ccgagcattt cgacttcgct acgctgttga ccatcgccca
     8521 gcagcgatgc cacgtttacc cccagatgct gccccagcag atccagcatt gccgcttcta
     8581 tcccggtaac tacatgaata gtggtacgca aatcaaatgt ctgcaaaccg cgcccactag
     8641 catcacgatc ggcaaaagta ttacgcacca gcgtcagaac gtttttgtat tcacccagcg
     8701 ttttacctac caccagcgga atcgcatctt ccagcgtttt acggattttc tcgccgccgg
     8761 gaatttcccc tacgccagtg tgaccggaat tatctttgat aatcacaata ttacgcgtga
     8821 agaacggtgc gtgtgcacca ctcagattca tcagcatact gtcatgaccc gccaccggga
     8881 tgacctgcat ttcagtaaca acaggcgtcg taaattgaga actcatattt atgtcctttt
     8941 tcagaatcaa tgacggccga aaacgggacg tttacggtca aaggtccagc cggggatcag
     9001 gtactgcatc ggacctgcgt cgttacgcgc accgcaaggc agacgtttat aggcctcatg
     9061 tgccttttgt acctgttccc agtccagttc cacgcccaga ccgggcgcat caggaacggc
     9121 aatttttccg tttttaatct ccagcggatt tttggtcagg cgacaatcgc cctcctgcca
     9181 aatccagtgg gtatcgatag cggtaggatt acccggtgcc gccgcgccca catgggtaaa
     9241 tatcgccaga gagatatcga aatggttgtt ggaatggcag ccccaggtca gcccccagtc
     9301 gtcgcaaagc tgcgccacgc ggactgcacc ggaaagcgtc cagaagtgcg gatcggcaag
     9361 tggaatatct accgcattga gcatcaccgc atgacccatt tcgcgccagt tggtggcgat
     9421 catgttagtc gcgacgggca agccggtcgc ccgtcgaaac tccgccatca cttcacgtcc
     9481 ggagaagccc tgttctgcgc cgcatggatc ttcggcatag gtaagaacat cattcagccc
     9541 tttgcataaa gaaatggctt catcaagcag ccatgcaccg ttgggatcaa cggtaatccg
     9601 cgcatccggg aagcttttct tcaatgcacg aacagtgtcg atttcttgct cgccaggtaa
     9661 caccccgccc ttaagtttga aatctttaaa gccatagcga tcctgtgagg cttccgccag
     9721 acgcacaacg gcttcgctgt tcatcgcttt ctgatggcgc aactgatacc actcatggtt
     9781 gcccggcgta ttttccagat aaggaagatc ggttttggtc cgatcaccta tataaaacag
     9841 ataaccgagg acggtaatag tctcgcgttg cttgcctggc cccaacagtt cgcagaccgg
     9901 aacattcagc gccttaccta gcaggtcaag caaggcggct tccagcgccg ccacggcatt
     9961 aacgcgcaat tcaaaagtcc aggcaccttt gccgaaggta tcaaaatcgg ctgcctgatt
    10021 acctttatgt acctgctgaa ccactttatt caggcgcgca acttcctggc ccagaaccat
    10081 cggaatagca tcgaccagtg tttgataaat cacctctccg cccggcgctt caccaatgcc
    10141 ggtatgcccg gcgttatcgg tgagtaccac gatattgcgg gtgaagtatg cgttatgcgc
    10201 accaccaata ttgagcaaca tgctgtcatg tccggcgacc ggaatgactt tcatatcagt
    10261 aataacagga ctggattgtg tcgccatctt attgccccgc gacaggtttc agctcgatac
    10321 gcttgatatc gcccaccagc accaggtagc tcagtaccgc gattagggca tgaacaccaa
    10381 cataaatcag cgccccatta aacgagccag tcgtgccaac gatataacca attgcgattg
    10441 gcgtgacgat gccagaaatg ttaccgaaca tgttgaacag gccaccggaa agaccgctga
    10501 tctcttttgg cgcggtatct gccattactg cccaacccag cgccccgatg cccttaccga
    10561 agaaggccag cgccataaag ccgatgatca tccactcaac gttgacgtag ttgcagaaca
    10621 ccatcaccat cgacagcaac atgcccatta cgatcggtgt tttacgcgca atgttcagcg
    10681 atcccgtgcg gcgcatcagc cagtcggaaa taattccacc cagcacaccg ccgataaaac
    10741 cgcaaaccgc cggaacggaa gccacaaagc ccgctttcag aatcgacatc ccacgcgcct
    10801 gcaccagata aaccggaaac caggtaatga aaaagtaagt cagggcgttg atacagtact
    10861 gaccgatata aacgccgatc atcatccgtg acccgagcag ctgtttgatc tgcccccact
    10921 tcacgctgaa cggcacttta actttggtgc tttgctgatc catattgatc agcgcgccac
    10981 ccgcggcgat atactccagc tctttctgat ttacccccgg atgttgattt ggctcgtgga
    11041 tgactttcaa ccagataaag ctgatgacaa tgcccagacc gcccataaag aagaagacgt
    11101 gtgaccagcc cacttcatgc gtcagccagc ccataatcgg cgcgaagatc accgttgcga
    11161 agtattgagc ggagttaaaa atcgacaccg ccgttcccct ttcctgcacc ggaaaccagg
    11221 ccgcaacaat gcgactgttg ccggggaaag atggcgcttc agcaagcccg accaggaagc
    11281 gcagagtaaa cagggcaacg ataatgccga atccactaaa gatatcgacg aagccttgca
    11341 gcaaggtaaa catcgaccag ataaagatcg accagaagta gacgcgtttt gaaccaaaac
    11401 gatccagcaa ccaaccacca gggatctgcc cgataacata agcccatgag aaggcagaga
    11461 acacatagcc cattcccacg ggatcaaggc cgatatcttt ggccatttcc gaaccggcaa
    11521 tagaaagcgt agcgcggtcg ccgtagttga aggatgtgac gataaacaac atcaccacta
    11581 tccagtaacg agcattcgtg cgtttttcca cactgctcgc agcctgactt aaagaactca
    11641 ttgttgcact cctgaaaatt cgcgttagcc acgctcactc tggactgcga catcgccagg
    11701 aaatcagagg tgacgtaggg tgttttttgc cgtttttata ggtcgttcgc cgaatacggc
    11761 gcgtgtttat atctggcaat agcagtataa aaagcgcgcc atagcggctc accgtgcaac
    11821 aacacaacat taatgcgttc aatgaggccc gattttggca ttagccctgg acggtggaat
    11881 ccacttcacg gaaatgaaaa caagaacaag aaaggaaggg taaaaacgaa gaaataaaga
    11941 agagtatgaa atggatcgct tgactctagg caaacgccag taaaaatccg cgctatgaag
    12001 cagtttttac tggcatttgc ctgaaaagat tcgattcagc accgctaaaa cgacatttac
    12061 cgctcgctga catatcagga caacagcgtg ccccactgtt cgacccacgg atttgattcc
    12121 gtttccggtt ccgggttctc actggcgtca atcaacagca tttcgccaac ccgctgagcg
    12181 ctctgttcct gcaacaaggc atcgaactgt ttgccgccat tgcaaaaatt tacataactg
    12241 ctgtcgccga gcgcaatcac gccataacgc agattcggct ggaagcccag actatctttg
    12301 attccctgaa agagcggcac aatgctatca ggaaggtcgc cctgcccggt cgtggacgta
    12361 accaccagaa catacttatc ctgatagggc agccagtcgc ttaattcagg atcttcaaat
    12421 accgttgctt tgtggccctg tgcggtcaga attgcttctg cttcttccgc cactaacagc
    12481 gaatttccgt acatggtgcc gacaaaaata ccaatttccg ccataccgtt ttctccctgg
    12541 attaggaact tatctctcca tcctgacccg atggcgcact gaactcaacc ctttcatttt
    12601 caggaagcag accgcgccag ccaaattgtg ataacgcctg catccaggtg tcgtccaggc
    12661 ccgcgtgaat agtcagcggc tcgccagtaa aaggatgcgt caatgacaac tgactggcat
    12721 gtagcattaa ccgctggagg ccaaaatgct cagcaccgct gcgattctgg cgtaaatcgc
    12781 catgtttgct atcgccaata atcggatgac gcaaatgggc aagatatcgg cgaagctgat
    12841 gtttgcgtcc ggttttcggc tccagttcca ccaggccgta gcgcgtggtc gggtaacgtc
    12901 cggtcgctac cggcatttct acggtcgcca gaccgcgata atgcgtcact gctggctgcg
    12961 ggcctttatc ttcgcgggca aatttatcag cgattttgtc cagttcttcc accagtggat
    13021 aatccaacac cgcttcttcc atcaaccagc cgcgcacaat cgcatggtaa cgtttctgga
    13081 tttggtgctg ttcaaactgt tgtgccagca gccgtccggc ctcgctggat aatcccatca
    13141 acaacacacc agaagtgggt cggtccagac gatgagcagt aaaaacatgc tgtcctatct
    13201 ggtcacgcac ggtttgcatg accactactt tctcgtcgcg atccagccag ctacggtgaa
    13261 ccagccagcc ggagggttta tttaccgcaa ccagccattc atcctgatag agtatttcca
    13321 gcattagctc gcatcatccg caaaaagagc atccagtttt tccaactcag ccagaataag
    13381 cgcgcgttgc ggatggtccg tcgccagcgc catttcataa tagggtgcaa cagcaaaagc
    13441 gcccggtaac ggctggttgt tatttaacaa atcgtgcatt cgcgggatca gcccccactg
    13501 caaccactcc agtggttcca tggtgtccat aaagaacggt tgggtactat taaattgatg
    13561 cggctggggt tcatcgtttc gccagtgctg atgttcacgc agtaacgctt caagcgcctg
    13621 caactggaga cgaacgcggt catgagtggt cataaagcaa cctcaataag aaaaactgcc
    13681 gcgaaggata gcaggaataa aaaaagggag cactgtattc acagtgctcc cggttcgttt
    13741 cgcagcattc cagctacttt tgttgctccc tgctcatcct tgacaacttt tcctctggcc
    13801 ttgcggccaa tcgttcatcc tgaactattg cttcctgctc acaccacccc gatgtgatac
    13861 ttcatcctga agtgtccctg gccttcctga cccaccgaat catcctgacc ggttctcatt
    13921 ctccatcctg gaggtgtcct ttaacgcgtc ctgcgtcatc ctcttcgctt catccagaag
    13981 cctttccctg aaacaccatc ctggtgtgtc ctgcagaagt gtcatcatcc tgatgttcac
    14041 ttcgttgtct gactccctgt cgacgaagat agaatcgtct ttttcgcgaa gtcttacaag
    14101 gcgcttacag acaatgcctt aaggaaaatt tcatatgaaa gttaagaatt aacaatcaac
    14161 attttgattt taaatgaaaa taacgattct tatcttttta agtattcgct tatttcctgg
    14221 cgatctccta cagagtctgt aagagatctc tcacaaccag atggggtatt tggattacag
    14281 aagaggctta agttgattaa ggaattccgc aagatttgag gctaaatgcg tccgtttacg
    14341 tgttcccagc gtctctttgc acacttcacc ggagagatta cacaccgaga tgacctctaa
    14401 ctcttcttcc agcgtcgcga taaatagcgt gggcggcagt tttagccttt tctgagtcac
    14461 cagatgacca atcagatttt cctgcacccg acggaagtcg tcttcactcc aggtttgcag
    14521 caatgtcagt ttgatatcgc caaactgcgc gtgcatatcc ccggcaaact gagtcgtata
    14581 aaaggtatga attgtgggtt gtatcacaat atcaaaagcg cgttcaaccg cgtttacatt
    14641 ttgttccccg gtaaacggct ggggttgcca gtatacggca tcttcggtag ttgaaataat
    14701 gcacggtgaa ggaacaccgt acaattcctc acttaacggc cagcttttat gctcttcatg
    14761 ccacgcatcg cagtagcgtg cagtaaaatc tttcagggct tgtgcggtca aatcgtccac
    14821 taatttctct cttcacttaa accagataca cttgtcgttt agtttatctg gtttatgacg
    14881 gtgaaacatg tcttcttatg caaaccatca ggcacttgcg ggcctgactc ttggaaaatc
    14941 aaccgattac cgggatacct atgacgccag cctactgcaa ggcgttccac gcagcctgaa
    15001 tcgcgacccg ctgggtctga aagcggataa cctgcctttt cacggtacgg atatctggac
    15061 gctgtatgaa ctttcctggc tgaatgcgaa aggtttgccg caggtcgctg tcggtcatgt
    15121 tgaacttgat tacaccagcg taaatctgat tgagtcgaag agttttaagc tctatctcaa
    15181 cagttttaac cagacgcgtt ttaataactg ggatgaggtg cgccagacgc tggagcgcga
    15241 cttaagcact tgcgctcagg gtaagattag cgtggcgtta tatcgtcttg atgaactgga
    15301 aggccagccg ataggtcatt tcaatggcac ttgcattgat gaccaggata tcactatcga
    15361 taactatgaa ttcactactg actatctgga gaatgccacc agcggtgaaa aagtagtgga
    15421 agagacgctc gtcagccacc tgctgaaatc aaactgcctg atcacccatc aaccagattg
    15481 gggttcgatc caaattcagt atcgtggacg ccaaattgac agagaaaaac tgctgcgtta
    15541 cctggtctca ttccgtcatc acaacgagtt ccacgaacag tgcgtggaac gcatctttaa
    15601 tgacctgtta cgcttctgcc agccagagaa attgagcgtt tacgcacgtt atacccgtcg
    15661 tggcggtctg gacattaacc cgtggcgcag taatagcgat tttgtcccat cgaccacaag
    15721 actggttcgg caataaattt tttctcaatt ttgcgtgctg gattcacgca gaaggttgtg
    15781 aaaggtcatc aggcagggct attgtaatca aagggaatga cgatattcgt cccataagga
    15841 gtttttcttg attacacata ttagcccgct tggctccatg gatatgttgt cgcagctgga
    15901 agtggatatg cttaaacgca ccgccagcag cgacctctat caactgtttc gcaactgttc
    15961 acttgccgta ctgaactccg gtagtttgac cgataacagc aaagaattgc tgtctcgttt
    16021 tgaaaatttc gatattaacg tcctgcgccg tgaacgcggc gtaaagctgg aactgattaa
    16081 tcccccggaa gaggcttttg tcgatgggcg aattattcgc gctttgcagg ccaacttgtt
    16141 cgcggttctg cgagacattc tcttcgttta cgggcaaatc cataataccg ttcgttttcc
    16201 caacctgaat ctcgacaact ccgtccacat cactaacctg gtcttttcca tcttgcgtaa
    16261 cgctcgcgcg ctgcatgtgg gtgaagcgcc aaatatggtg gtctgctggg gcggtcactc
    16321 aattaacgaa aacgagtatt tgtatgcccg tcgcgtcggg aaccagctgg gcctgcgtga
    16381 gctgaatatc tgcaccggct gtggtccagg agcgatggaa gcgccgatga aaggtgctgc
    16441 ggtcggacac gcgcagcagc gttacaaaga cagtcgtttt attggtatga cagagccgtc
    16501 gattatcgcc gctgaaccgc ctaacccgct ggtcaacgaa ttgatcatca tgccggatat
    16561 cgaaaaacgt ctggaagcgt ttgtccgtat cgctcacggc atcattatct tccctggcgg
    16621 tgtgggtacg gcagaagagt tgctgtattt gctgggaatt ttaatgaacc cggccaacaa
    16681 agatcaggtt ttaccattga tcctcaccgg cccgaaagag agcgccgact acttccgcgt
    16741 actggacgag tttgtcgtac atacgctggg cgaaaacgcg cgccgccatt accgcatcat
    16801 cattgatgac gccgctgaag tcgctcgtca gatgaaaaaa tcgatgccgc tggtgaaaga
    16861 aaatcgccgt gatacaggcg atgcctacag ctttaactgg tcaatgcgca ttgcgccaga
    16921 tttgcaaatg ccgtttgagc cgtctcacga gaatatggct aatctgaagc tttacccgga
    16981 tcaacctgtt gaagtgctgg ctgccgacct gcgccgtgcg ttctccggta ttgtggcggg
    17041 taacgtaaaa gaagtcggta ttcgcgccat tgaagagttt ggtccttaca aaatcaacgg
    17101 cgataaagag attatgcgtc gtatggacga cctgctacag ggttttgttg cccagcatcg
    17161 tatgaagttg ccaggctcag cctacatccc ttgctacgaa atctgcacgt aatctcccct
    17221 ccc
//
LOCUS       DAJTQY010000080        17176 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.080, whole genome shotgun sequence.
ACCESSION   DAJTQY010000080 DAJTQY010000000
VERSION     DAJTQY010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17176
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.080"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            <1..188
                     /locus_tag="OO349_002866"
     CDS             <1..188
                     /locus_tag="OO349_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HCR5867867.1"
                     /translation="YEWQKDVDLHQVLWSRSRLGERPKGQGITGADHFWFGHTPLRHR
                     VDIGNLHYIDTGAVFGG"
     gene            complement(213..404)
                     /locus_tag="OO349_002867"
     CDS             complement(213..404)
                     /locus_tag="OO349_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HCR5867868.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(509..745)
                     /locus_tag="OO349_002868"
     CDS             complement(509..745)
                     /locus_tag="OO349_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HCR5867869.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            complement(863..2302)
                     /gene="rsmF"
                     /locus_tag="OO349_002869"
     CDS             complement(863..2302)
                     /gene="rsmF"
                     /locus_tag="OO349_002869"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HCR5867870.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTTEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASWVKVLHA
                     NISRCGISNVALTHFGGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNREENEAVCRWLKETYPDEVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQAAASVGLNWDGNLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIAHASPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTSNA"
     gene            complement(2382..5002)
                     /gene="yebT"
                     /locus_tag="OO349_002870"
                     /pseudo
     CDS             complement(2382..5002)
                     /gene="yebT"
                     /locus_tag="OO349_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
     gene            complement(4971..6254)
                     /gene="yebS"
                     /locus_tag="OO349_002871"
     CDS             complement(4971..6254)
                     /gene="yebS"
                     /locus_tag="OO349_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HCR5867871.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDAITGSMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGLCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            6384..6881
                     /gene="msrC"
                     /locus_tag="OO349_002872"
     CDS             6384..6881
                     /gene="msrC"
                     /locus_tag="OO349_002872"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HCR5867872.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFARFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            6978..7676
                     /gene="proQ"
                     /locus_tag="OO349_002873"
     CDS             6978..7676
                     /gene="proQ"
                     /locus_tag="OO349_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HCR5867873.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            7696..9744
                     /gene="prc"
                     /locus_tag="OO349_002874"
     CDS             7696..9744
                     /gene="prc"
                     /locus_tag="OO349_002874"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HCR5867874.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNAQWDSKVKFDELSLKLTGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLSKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            9936..10817
                     /gene="htpX"
                     /locus_tag="OO349_002875"
     CDS             9936..10817
                     /gene="htpX"
                     /locus_tag="OO349_002875"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HCR5867875.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(10863..12236)
                     /locus_tag="OO349_002876"
     CDS             complement(10863..12236)
                     /locus_tag="OO349_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5867876.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMATGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRARA"
     gene            12413..13204
                     /gene="kdgR"
                     /locus_tag="OO349_002877"
     CDS             12413..13204
                     /gene="kdgR"
                     /locus_tag="OO349_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HCR5867877.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLQCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(13347..13586)
                     /locus_tag="OO349_002878"
     CDS             complement(13347..13586)
                     /locus_tag="OO349_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5867878.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLEITDCPLLRKSNIVVDNG"
     gene            13745..13888
                     /gene="mgrB"
                     /locus_tag="OO349_002879"
     CDS             13745..13888
                     /gene="mgrB"
                     /locus_tag="OO349_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HCR5867879.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            13963..14250
                     /locus_tag="OO349_002880"
     CDS             13963..14250
                     /locus_tag="OO349_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HCR5867880.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
     gene            14829..14972
                     /locus_tag="OO349_002881"
     CDS             14829..14972
                     /locus_tag="OO349_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HCR5867881.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            14985..15194
                     /gene="cspE"
                     /locus_tag="OO349_002882"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             14985..15194
                     /gene="cspE"
                     /locus_tag="OO349_002882"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCR5867882.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            15360..16169
                     /gene="rlmA"
                     /locus_tag="OO349_002883"
     CDS             15360..16169
                     /gene="rlmA"
                     /locus_tag="OO349_002883"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HCR5867883.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(16166..16732)
                     /gene="mntP"
                     /locus_tag="OO349_002884"
     CDS             complement(16166..16732)
                     /gene="mntP"
                     /locus_tag="OO349_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HCR5867884.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGAPLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIITTALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
BASE COUNT         4532 a         4154 c         4337 g         4153 t
ORIGIN      
        1 tttatgaatg gcaaaaggac gttgatttgc atcaggtctt gtggagccgc tcgcgattag
       61 gtgaacgccc aaaagggcag ggaattacag gtgctgatca tttctggttt ggtcatacac
      121 cgttgcgaca tcgcgtggat attggcaacc tgcattatat tgataccggt gctgtctttg
      181 ggggctaact gactcttgtg caactgcaat aattaaaaat caccatactc ctgtgctggt
      241 cgccagaaac catctataaa atcctcaatc ggaaaacaac cgccatggcg gatccgttga
      301 tcgctcatag aataaagaca ctgctgttcc gtgttgtaga catccacaac aatatcttca
      361 caaccgccat ctaggtagca aacaaaaagt accagcgcga acatttcatc cccgaagtgt
      421 ggtgccgtac cgttaagttt aggagagatt ttacaacggg ggaataacca ggacaaataa
      481 cccgccatgc cgacgggttc tttttggatc aggcaagacg cataatccag gcatcagatt
      541 gttgatcgta acgggtacgg taaaggacag agtgttcgcc atttagcagg gcagggatac
      601 tggtttcagc atcccaggca tccagttgca tacataaatc ggggtcagat ttacaaggaa
      661 tggttaacgt tcgatcaccc ggcgattcac cgtgtaattc gccgtcgtcg atttcaaaag
      721 cgcctatgcg cacactggtt ttcatcttcg tgctctccat ttagctgctg attgtagtac
      781 gaccagtccg atcgcacaat ttttcagcct agttgaggcc ggcgaatgtg ccagtcaaaa
      841 agtgcgcttt ttttgtgcgc tgtcatgcgt tactggtaaa aagtttcccg tcccgcacca
      901 gttcacgcgg gtagctgttt ttcagtcgcg aaccaatccg tttggctaaa ccaatcggct
      961 gatgctggaa agtaaccaac acgtcatccg ccactggcgc ggcttgcggg taaacatcgc
     1021 gcccgcgata ccactcctcc gcttcctgcg gtgtcagttc aaaagcgttt acattgtcgg
     1081 gggaggcatg ggcaataacc gcttcatgct gccagcgata acctttgtta tgcgtttcgg
     1141 caagtttaat ccctaagcga gaaaatcgga ctttaccgat cagggcttca atgcctaccg
     1201 ggaacaacca cagttctttg tcgcgttgcc agagtcgcag gtttccatcc cagtttaagc
     1261 caacacttgc agccgcctga cgaatttgtc cggcttcgcg atctttcacc gggctgaacg
     1321 ggaaattgcc cactttgtat tttggggcgg gtaaggcggg gatcgcctga gttttacgca
     1381 gacgagcaac gaagaagcct tcgcagtcgt aaatttgtgg gaaaacatgc aaaaaacctt
     1441 cttcggtcag cgccttgttt gcaccaggga agagatcgcc aagtggtaaa aactctactt
     1501 cgtcggggta agtctctttc agccaccggc aaacggcttc gttttcttcc cggtttaagg
     1561 tacaggttga gtaaaccagc gtaccgccag gacgtaatgc atgaaaggcg ctgtcaatca
     1621 gttcccgttg ggtagctgcg atttcctgat tgctttcggg tgaccagttt tttagcgcat
     1681 cgggatcttt acgcaccacg ccttcgccgg agcagggagc gtccagcaaa atggcatcaa
     1741 acatttctgg cactgccgca ccaaacacgc ggccaccaaa atgtgtgagc gcaacattac
     1801 tgatgccaca gcggctgata ttggcatgta acacttttac ccaactggcg gaaaactcat
     1861 tggcaaggat tgccccttcg ttattcatcc gcgcggcaat ttgcgtcgtt tttgagcctg
     1921 gcgcagcagc gacatccatc acccgctgtg gtgcattacc gtcagcaaac aaggcggcga
     1981 cgggcaacat tgaactggct tcctgaatat aaaacaggcc acttaaatgc tcggtggtac
     2041 tacccaatgg caatgcatct tcattgtcgc gttcaatcca gaaaccttct tcacaccacg
     2101 gaattggcgt aagcgtccag ccataaggag cggttaattg caggaaatca gcaacggaga
     2161 ttttcagcgt attaacgcga atgctgcggc gcaacgggcg ctgacaggcg gcaagaaaat
     2221 catcaaatga gagcgtcgaa ggcatagctt cgcgcatttg tgtcagaaac gcgtccggga
     2281 aataaacggt gtgttgggcc acgagcatgt accactggca aaaaaacagg cgcgcagttt
     2341 agcataagcg ctccggcgca ggcacgccgg agcagtgggc attatttggg aagcgcagtt
     2401 ccccattcac gccactcttt cggttcactt tcctgcaaca ggaagtgttt gccttcttgg
     2461 gctttcggtg caagtggcgt acccggaggc gtggcgaagg cgatgccgcc acggataaat
     2521 tggttaaagg tgccggtttt cactacgccg cccgtcagac caaagtccag actgtaaccc
     2581 gatgccaacc agaagacgga attgttacgc accaggtgtt gatagcgttt actgatgcgc
     2641 atcgcaatca tcacgcgatc tgacaatgtc cccagcgtca ttccggtaac cgtacctact
     2701 tccagaccac ggaacagcac aggcgtaccg atgcctaacg aaccggcttc cggcgcttca
     2761 acaataatgc ttaaaccatc taggtaacgc gaatcagtaa tggtggcctc ttctaattca
     2821 aagtcgcgac gaggattgcc acggcctggt tcgacgttga tatacggctg gaggatagta
     2881 tctagatgct caacgccagc tgccgagatt tgcggtgtga ccactgagaa gcgcgtacca
     2941 ccgcgagcaa aggtctggac atattccgga tagagcaccg cctttgcctg tacttcattg
     3001 cgtgcggtaa tcagatccag cgtctggatt tgcccgatat caataccgag atagcgaatt
     3061 ggcatcccga ccgccagttt tccggcatcg aaagcgtgaa gcgtaatctg cctgccaacc
     3121 gcacgggccg ctgtttcgga agcatacaga attcgcttgt cgcctttacg ctgactggcg
     3181 ctggcaccac tgaggttatc gaagctaatg gcccccttta atgctctgga gagcggggat
     3241 gcctgtacgg tcaggccact accattcagc tgaactttcg ccccgccttc tgcccagaac
     3301 acgctgttgc tggtcaaaag gttgcgatat tccggtttaa tgtgcagatc gatatcgaac
     3361 gcgttagctc gcggacgcac ggtaataact tcaccaactt caaatttacg gtacagcact
     3421 accgatcctg cctgcacatc cggcagcgtc tttgcactca aactcacggt tgtggtgggt
     3481 aaatcgctaa ggctgttctc cagcgctttt tccagattgg catacagtgg atagctggct
     3541 ttcatctcgc ctttatcgcc cggcagaata cgtatcccgc cgttaatcca ttctgaggcg
     3601 ctggcaccga gaaactcaac gccatccagc cccaccttca cgtcgacacg gctgttgacg
     3661 acaaatttgc tatcgccttt taccagttct cgatgctgag gctcgatggc gacggtaaag
     3721 gtgacgcctt tgctggtgag tttacgatcg ataacctggc ctacctgcac gccgtgaaga
     3781 atgagcggct gacccgcatc aataccgtaa ctttccggtg ctgtcagggt cagtgtcaga
     3841 acgtccggct cttgcagcag ggctttttcg cccggcacaa caacgaactc tttgcgtggc
     3901 tcgccatcgc cgggcaccaa ctcaaaggtt tttccggtca gcagggcgct gagattagca
     3961 ttgctaaggg ataatttcgg gttgcgtaat tcgatgcggg tattttcacg aagcagggat
     4021 caacggtcat ttccccagtg actttaccac caggatttaa atccagttta gtcagctgtc
     4081 cgacttccag cccctgatac attaacggcg tcgagtcggc ggttaatcct gctccactcg
     4141 gcagttccag ttttattatt actccacgct ggctgtgggc cagatcttca tacagaccaa
     4201 aggtatcttc cgcctcggca ggtttcgact cttcaggtga atcgaaggca atcgcaccgt
     4261 taaccagtgc cgccagactt tccagtttca ccttcgcgcc actgatactg acgttggcat
     4321 caacgccgga aacgtttcag aaacggctac cttttttcac cagatcggta aaacgccgct
     4381 cgatcaggac atcaatcacc acgccttgct tgttgggatt gatggcatag tcgtagactt
     4441 ttcccaccgg gatcttgcgg aaatagacca atgaaccgct gttcagcgaa ccgagagcgg
     4501 gggcttgcag gtggatcatc agatcgccat tgtccagccg atatttcggt tgggtatcga
     4561 gcgcgacaaa gtgatcctgc tctttacctt tacccggcat catgccgata tagttcccac
     4621 cgacgagggc gtccagcccg gagacacctg ccaacgatgc ttttggcgtc atcagccaga
     4681 actgagtctc ttcgcgcagc gcatctttca tatcggactt gatgctgacc ttgacttcaa
     4741 tcttacgaag atcgtcgctg aggctgatat cctgcactgt tccgacttca acgccctgat
     4801 aacgaacagg cgtacggccc ggaacaatac catccgccga cataaagtcg atggtgacgg
     4861 tattaccccg gtcctgataa ctgtcccaaa tcagccaacc ggcaatcatt agcgcgatga
     4921 aaggcagcag ccagaaaggt gagatacggc gtttattttt aatctgcgct tcagtcgtcg
     4981 aagcgggcgt ttcctgactc atgtgcatcc caaagtaagc ggctgtccag ccattccaca
     5041 gcaagaatag tcaaaattac ggcagcgcca aaataaaacg cagccggtcc catagtaaaa
     5101 gcgaggatct gatcgcgatt aatcagcgac atggttaaag atatgacaaa caggtcgagc
     5161 atcgaccagc gtccaatcca ggtcaccatc cgcagtaaca gaatgcgtgt gcgtaaacct
     5221 tgctggcatt taaaatgaat gctcaacagt aaagtgaaca tgacgatcac tttagtaaac
     5281 ggtaccagaa tactggcgat aaacacgatt cccgcaaccg caatgttgct acttgccagc
     5341 gacataattc cggaaagaat cgtatcttcc tgccggccac cattcagata aatgatagaa
     5401 ataggcaata ggttggctgg taacaacaaa acgattgacg ctaacagcgc cgcccagcat
     5461 ttttgcaaac tatgacgacg acgcaggcgt agcgggatat ggcaacgcgg gcagagacca
     5521 cgctgatcag gatagccggt aaaatggcac ccaagacaga cacgaagttt ctcgtcccta
     5581 cgcgtagcgg ggcgctgcgg ataatatcgc tcccacagct cctcaacatt aagatgtgac
     5641 aacgtcaccg tcgttaaaat caccaacgcc acaaaagaga acaagccgac acccgcctgg
     5701 atatgggcat aatcctgtac ctttatagaa gcaacgccaa tgccgaccag gtagatatcc
     5761 agcattaccc actctttaag tcgctcaagc atcagcagta ccggacgtaa attcattccc
     5821 agtcggttac caaaccataa atatgctatg gaggacacca gaatgagggg ggcaccaata
     5881 acgcagaaaa agaccattga tcccgttatc gcatcgccct gtttggtcat ttgccagatg
     5941 ccttgcatga cgttggcgtc aatacgaatg cctaacagcc agatatgcaa cagcggttcg
     6001 ccccaggcaa acggcataag caacagcatg gtgaaggcca ttgccgccag gcgcgttagc
     6061 gaccagtcgc gcccgtcgcg aatttttgcc tgacagcgcg gacaataggc actttgatga
     6121 gaatttatct cgggcaggct aaacagcatg tcacattgcg ggcaacgttg gtaatcacca
     6181 cgcggcagtt cctcgccgat tgccctcact gttatctttt ttgtcggcgt aatttgtggt
     6241 gtgttaagag ccatgacgtg ccaaatgtaa taagaattaa cattaatctt aacccatgat
     6301 gggaatgatc ttgagcatta acgcatttaa tgcttatttt aataggctgg cagttccagg
     6361 taagacaacc aagtgattaa gtaatgaaca aaacagaatt ttacgcggat ttaaatcgcg
     6421 actttaacgc gctgatggcg ggagaaacca gttttctggc aacgcttgcg aacaccagtg
     6481 cgttgttata cgagcgtctc actgacgtta actgggcagg tttttatttg cttgaggacg
     6541 atacactggt actcggacca tttcagggca aaattgcctg tgtccggata cccgtcgggc
     6601 gcggggtgtg cggcacagcg gttgcccgca atcaagtgca gcgtatcgag gatgttcacg
     6661 cgtttgacgg gcatattgcc tgtgatgcgg cgagtaattc tgaaattgtt ctgccgctgg
     6721 tggtgaaaaa tcagattatt ggtgttctcg acatcgatag taccgtcttc gctcgcttta
     6781 cagacgagga cgagcaaggc ttacgtcagc ttgtggcaca gcttgaaaaa gtgcttgcaa
     6841 cgacggatta caaaaaattc tttgcgagcg tcgcaggata atcaacggat aacgtagcat
     6901 ttactggtag cgtcattata atgacgcctg ttcatgcctg cgcttgttgg ctacgtccgt
     6961 tgtaatcagg aaatttcatg gaaaatcaac ctaagttgaa tagcagtaaa gaagtaatcg
     7021 cgtttctggc cgaacgtttt ccccactgtt tcagtgcgga aggtgaagcg cgtccgctga
     7081 aaattggtat ttttcaggat ttggtcgatc gtgttgccgg ggaaatgaac ctgagcaaaa
     7141 cgcaattacg atccgcttta cgtctctaca cttcgagctg gcgttatctt tacggtgtta
     7201 aacccggcgc aacgcgtgtc gatcttgacg gcaacccatg cggtgagctg gacgagcaac
     7261 atgtagagca tgctcgcaag cagcttgaag aagcgaaagc gcgtgttcag gcacagcgtg
     7321 ctgaacagca agcgaaaaaa cgcgaagctg ccgcagctgc tggtgagaaa gaagacgcac
     7381 cgcgccgcga acgcaagcca cgtccgacca cgccacgccg caaagaaggc gctgaacgta
     7441 aacctcgtgc gcaaaagccg gtagagaaag cgccaaaaac agtaaaagca cctcgcgaag
     7501 aacagcacac cccggtttct gacatttcag ctctgactgt cggacaagcc ctgaaggtga
     7561 aagcgggtca aaacgcgatg gatgccaccg tattagaaat caccaaagac ggcgtccgcg
     7621 tccagctgaa ttcgggtatg tctttgattg tgcgcgcaga acacctggtg ttctgaaacg
     7681 gaggccgggc caggcatgaa catgtttttt aggcttaccg cgttagctgg cctgcttgca
     7741 atagcaggcc agaccttcgc tgtagaagat atcacgcgtg ctgatcaaat tccggtatta
     7801 aaggaagaga cgcagcatgc gacggtaagt gagcgcgtaa cgtcgcgctt cacccgttct
     7861 cattatcgcc agttcgacct cgatcaggca ttttcggcca aaatctttga ccgctacctg
     7921 aatctgctcg attacagcca caatgtgctg ctggcaagcg atgttgaaca gttcgcgaaa
     7981 aagaaaaccg agttaggcga tgaactgcgt tcaggcaaac tcgacgtttt ctacgatctc
     8041 tacaatctgg cgcaaaagcg ccgttttgaa cgttaccagt acgctttgtc ggtactggaa
     8101 aagccgatgg atttcaccgg caacgacact tataaccttg accgcagcaa agcgccctgg
     8161 ccgaaaaacg aggctgagtt gaacgcgcag tgggacagta aagtcaaatt cgacgagtta
     8221 agcctgaagc tgacaggaaa aacggataaa gaaattcgtg aaaccctgac acgccgctac
     8281 aaatttgcca ttcgtcgtct ggcgcaaacc aacagcgaag atgttttctc gctggcaatg
     8341 acggcgtttg cgcgtgaaat cgatccgcat accaactatc tttccccgcg taataccgaa
     8401 cagttcaaca ctgaaatgag tttgtcactg gaaggtattg gcgcagtgct gcaaatggat
     8461 gatgactaca ccgttatcaa ttcgatggtg gcaggtggtc cggcagcgaa gagtaaagct
     8521 atcagcgttg gtgacaaaat tgtcggtgtt ggtcaaacag gcaagccgat ggttgatgtg
     8581 attggctggc gtcttgatga tgtggttgcc ttaattaaag ggccgaaggg cagtaaagtt
     8641 cgtctggaaa ttttacctgc tggtaaaggg accaagaccc gtactgtaac gttgacccgt
     8701 gaacgtattc gtctcgaaga ccgcgcggtt aaaatgtcgg tgaagaccgt cggtaaagag
     8761 aaagtcggcg tgctggatat tccgggcttc tatgtgggtt tgacagacga tgtcaaagtt
     8821 caactgcaga aactggaaaa acagaatgtc agcagtgtga tcatcgacct gcgtagcaat
     8881 ggcggtgggg cgttgaccga agcggtatcg ctctcaggtc tgtttattcc tgcgggtccc
     8941 attgttcagg tccgcgataa caacggcaag gttcgtgaag acagcgatac cgacgggcag
     9001 gtgttctata aaggcccgct ggtggtactg gttgaccgtt tcagtgcttc ggcttcagaa
     9061 atctttgccg cggcaatgca ggattacggt cgtgcgctgg ttgtgggtga accgacgttt
     9121 ggtaaaggca ccgttcagca ataccgttca ttgaaccgta tttacgatca gatgttacgt
     9181 cctgaatggc cagcgctggg ttctgtgcag tacacgatcc agaaattcta tcgcgttaac
     9241 ggcggcagta cgcaacgtaa aggcgtaaca ccagacatca tcatgccgac gggtaatgaa
     9301 gaaacggaaa cgggtgagaa attcgaagat aacgcgctgc cgtgggatag catcgatgcc
     9361 gcgacttatg tgaaatcagg agatttaact gcctttgaac cggagctgct gaaggaacat
     9421 aatgcgcgta tcgcgaaaga tcctgagttc cagaacatca tgaaggatat cgcgcgcttc
     9481 aacgctatga aggacaagcg caatatcgtt tctctgaact acgctgtgcg tgagaaagag
     9541 aataatgaag atgatgcgac gcgtctggcg cgtttgaacg aacgctttaa acgagaaggt
     9601 aaaccggagt tgaagaagct ggatgatcta tcgaaagatt accaggagcc ggatccttat
     9661 ctggatgaga cggtgaatat cgcactcgat ctggcgaagc ttgaaaaagc cagacccgcg
     9721 gaacaacccg ctcccgtcaa gtaatatcaa tcaggcacaa gaaattgtgc ctgatttttt
     9781 aacagcggca agatgccgta aatcagatgc tacaaaatgt aaagttgtgt ctttctggtg
     9841 acttacgcac tatccagact tgaaaatagt cgcgtaaccc atacgatgtg ggtatcgcat
     9901 attgcgtttt gttaaactga ggtaaaaaga aaattatgat gcgaatcgcg ctcttcctgc
     9961 taacgaacct ggccgtaatg gtcgttttcg ggctggtact gagcctgaca gggatacagt
    10021 cgagcagcgt tcaggggctg atgatcatgg ccttgctgtt cggttttggt ggttccttcg
    10081 tttcgcttct gatgtccaaa tggatggcat tacgatctgt tggcggggaa gtgatcgagc
    10141 aaccgcgtaa cgaaagggaa cgttggctgg tcaatactgt agcaacccag gctcgtcagg
    10201 cggggatcgc tatgccgcaa gtggctatct accatgcgcc ggacatcaac gcttttgcaa
    10261 ccggtgcgcg ccgtgatgcc tctctggttg ctgtcagcac cggtttgctg cagaacatga
    10321 gcccggatga agccgaggcg gtaattgctc acgaaatcag ccacatcgcc aatggtgata
    10381 tggtcaccat gacgctgatt cagggcgtgg tgaacacctt cgttatcttt atttcccgta
    10441 ttctggcgca gcttgccgcg ggttttatgg gcggaaatcg tgatgaaggt gaagagagca
    10501 acggcaaccc gctgatctac tttgcggttg caacggttct ggaactggtg tttggtattc
    10561 tggcgagcat tatcaccatg tggttctcgc gtcatcgtga attccatgct gatgccggtt
    10621 cggcaaaact ggttggtcgc gagaaaatga ttgccgcgct gcagcgcctg aaaaccagct
    10681 atgaaccgca agaagcaacc agcatgatgg ctttctgcat taacggtaag tcgaaatcgc
    10741 tcagtgagtt gttcatgact cacccgccgc tggataaacg tattgaagct ctgcgtacgg
    10801 gtgaatacct gaagtaatat gaagtaaata ctaaaaagcg cgtcctgatc gacgcgcttt
    10861 ttttatgccc tggctcgtgg ctgagtgata cgtaaaccac tgacacaggc agcaatcact
    10921 gccagaatag ccgcagccat cagcgagacg tgcgtaccat tatcaccaaa ctgatttagc
    10981 atcagcgcca ccagcgccgc gccactactc tgacccagta gacgagccgt tcctaacatg
    11041 ccactggccc caccgctacg ttcgcgcgga gcggaggtaa taatggtgtg gttattgggt
    11101 gactggaata agccaaatcc agcgccacat aagatcatcg gccagataat attgatatcc
    11161 gcaggtgacg cgggcagcag aaccagggaa aaaagccccg tagccatgat gaacaaccct
    11221 aaagcaccca gcaatcctgc atgtacgcgt tcaatcaaat agcctgccag cggagccatc
    11281 accatcgttg ctaacggcca cggtgtcaga agtaaacctg tttcgacttc actacgcccg
    11341 agcacggttt gcaggtaaaa gggcagggaa accattgcca gcatttgtgc gcagaaagag
    11401 caaacagatg tgcaaataga aagtgaaaac agcgggatac gcagtaaatc caccggtagc
    11461 agcggtacgg gaagagaaag ctggcggcga ataaagaaaa taccaacaac aaccattacc
    11521 accagttccg cagcaattaa cgtcagcgat tgcccctgag cgaaaccact caacgcagtg
    11581 ataagcaggc cgaaggttaa cgcgttcatc acggcgctgg gcaggtcgaa acggggttta
    11641 ctggcgcgag aaccattggg tggcagaaaa cgcatcgcca gaagcagggc gataatacct
    11701 aacggtacgt tgattaaaaa taaccatttc caggatgcga tggagaggat tgctgcagca
    11761 attgtcggcc cggcagcaga agagacggca acaataaacg agtttatgcc catccctctc
    11821 cccagaaaac gttgtggata gatcaggcgg ataagtgcgg tattaacgct catcaacgct
    11881 gcaccgccga aaccttgtat gacacgcgca agggtgagca tttgcagcga atcagaaagg
    11941 gcgcagaaca gtgaagacaa cagaaaaacg accagaccgc atttataaat acgtcgatag
    12001 ccaaacatat cgcccagaaa cgaaaacgag agcagggaga tgacaatggc gatttgatag
    12061 gcgttcacta cccagatgga actggctggt gtggcatgaa ggtccgtggc aattgttggc
    12121 agggcgacgt tggcgattgc gccgtcaagg acagccatcg aaataccaat cacaatggtt
    12181 aatatcgcac cgtatcgctg gggcaatggc aggccgtcgg cctgaacttt tggcatagga
    12241 attttatatc tttggtgaat aatcaacagg ggtatgattc tagcactgat attcacggtg
    12301 gatgtcgcag aattgtaact aaccggcaaa agatggattg cgaccaccct ggtgcgaaat
    12361 tataataaaa aacagttctg atttttataa aacactcgca atgaggtgat aaatggctaa
    12421 cgcagatctg gataaacagc ctgattctgt atcttccgtg ctaaaagttt ttggcatttt
    12481 gcaggcgctg ggtgaagagc gcgaaatagg gataaccgag ctgtcgcagc gcgtcatgat
    12541 gtcaaaaagc accgtttatc gctttttaca gaccatgaaa accttaggtt atgtggcgca
    12601 ggaaggggag tcggagaaat attccctgac cctgaaattg tttgaactgg gcgctcgcgc
    12661 gttacaaaac gtcgatttaa ttcgtagcgc agatatccag atgcgtgaga tctcccgcct
    12721 gaccaaagaa actatccacc tcggcgcact ggacgaagac agtattgttt acattcacaa
    12781 aattgactct atgtacaatt tgcgcatgta ttcacggatt gggcgtcgta atccgctgta
    12841 cagcaccgcg attggtaagg tactgctggc atggcgcgat cgcgatgaag tgaagcaaat
    12901 tcttgagggc gtggagtata aacgcagtac cgagcggacc atcaccagta cagaagcgtt
    12961 attacccgtt ctggaccagg tgcgcgagca ggggtatgga gaagataacg aagagcagga
    13021 agaagggctg caatgcattg cggtaccggt atttgatcgc tttggcgtgg tcattgccgg
    13081 tttgagcatc tccttcccga cgttgcgttt ctctgaagag cgtttacagg aatatgtcgc
    13141 aatgttgcat accgcagcgc gcaaaatttc tgcccaaatg ggttatcacg actatccgtt
    13201 ctgatgagag taagaacctg tcggaatatc aaacagacag gttctttatt tagcatgaga
    13261 aaaataaagt tgaaggtgcg ttatattaaa cgcgcttgct ataagaatat tttactcatg
    13321 agtgagaatc tggttattta ttacccttaa ccattatcga ccacgatatt gcttttacgt
    13381 aacagtgggc aatctgttat ctccaagaaa ccacttttag tgtgcaagta ttgtaccgtg
    13441 ctggtgcctc tggcagtcag ataggtacat tgcaaaccta atcctgcggc attctctttg
    13501 cttccaatca aaacgccata tccgctgagt aataatccta tccataccag tgctatcagc
    13561 ataactgtgc gaatgatgaa tcgcattaca atctcttctc tttttatgtt cgcttaatcg
    13621 tagcggcaat atgcgctgaa gcaagctact cattccgaaa aagcacgaat atcgacatag
    13681 ttaggcgctg tttaactaac gcatgctagt ttaatgacat aaggtaggtg aagcggagat
    13741 tggagtgaaa aagtttcgat gggtcgttct ggttgtcgtg gtgttggctt gcttgctgct
    13801 ttgggcgcag gtattcaaca tgatgtgcga tcaggatgta caatttttca gcggaatttg
    13861 tgccattaac cagtttatcc cgtggtgata tcattttttc agcactagat tttcttcctc
    13921 tccagcagtg gtagaatagc gcctctactc agaggtggtg aaatgaacga agttgtaaat
    13981 tcaggcgtga tgaacattgc gtctttggtt gtatcggtgg tggttcttct tatcgggctc
    14041 atcttgtggt tttttatcaa tcgtgccagt tcacggacta acgaacagat tgaactgctt
    14101 gaggcgttgc tggatcagca aaaacgtcaa aatgcactgt tacgtcgttt gtgcgaagca
    14161 aacgaaccag agaaagcaga taaaaagacc attgagagtc aaaaatcggt tgatgacgaa
    14221 gatattattc gcctggtcgc cgaacgataa tccttcctca atcttgagta accccgtcgt
    14281 tgaagggtta ctcaattcat cctgattata cgcactgtca tggtgcaact gcttcaaatt
    14341 aaaactctgg tctgacttca aataattatc atattattta ttagcgaaac gtttctctct
    14401 tgattttttt gctcactcat caatttttct tattttaaat ttacaatccc tttggggatt
    14461 gacttctctt tagggtaatt gatagccgtt aactgactgt tttatgagaa aaagtgatat
    14521 aactttttat tcattgcata gcagaaaatg tgatattgca cgcactatgt aataacttct
    14581 ccctctggcc tggaacaact gaacttattg aactatgtta gaaactacgc cagtttaagt
    14641 atctgcctga actggcaagg ttaagcacaa tgatatatcg gcgcgtattc cgttgcataa
    14701 gtgtgcaaaa aaagtggaag acgtatcgag atttgtgcgt ctgatcgaga catgtttaaa
    14761 aatggcttgc cataattaac gttgtatgtg ataaaagatt tcgggttaaa cgaggtacag
    14821 ttctgtttat gtgtggcatt ttcagtaaag aagtcctgag taaacacgtt gacgttgaat
    14881 accgcttctc tgccgagcct tatattggtg cctcatgcag taatgtgtca gttttatcta
    14941 tgttatgcct gcgggcgaag aaaacaatct aaggaatttt tcaaatggca aagattaaag
    15001 gtcaggttaa gtggttcaac gagtctaaag gttttggctt cattactccg gctgatggca
    15061 gcaaagatgt gttcgtacac ttctccgcta tccagggtaa tggcttcaaa actctggctg
    15121 aaggtcagaa cgttgagttc gaaattcagg acggccagaa aggtccggca gctgttaacg
    15181 taacagctat ctgatcgaat ccactgatct gaagtgtgaa tacgcttcaa tctcgctata
    15241 aagcctcgtc gaatgcgagg ctttttacta tgctttatct tcgctcctgg cgttcggata
    15301 tttgcccgcc gcgtgattcg cgttacactt gcggccttta gtatcctgct ggagttgtca
    15361 tgtcgttttc ctgtccactt tgccatcagc ctctttcgcg tgaaaaaaac agctatatct
    15421 gtccccaacg acatcagttt gatatggcga aagaagggta tgtcaatctg ctgcccgttc
    15481 agcataaacg gtctcgtgat ccgggcgaca gcgcggaaat gatgcaagca cgccgcgcat
    15541 tcttagatgc cggacattat cagccgctgc gtgatgcaat tgtcgcccaa ctgcgggaac
    15601 ggcttgatga aaaggccacg gcggtgctgg atattggctg tggtgaaggg tattacacac
    15661 acgcatttgc cgatgcgttg cccgaaatca ccacgtttgg cctggatgtc tcgaaggtag
    15721 cgataaaagc ggcggcgaaa cgctatccgc aggtcactct ttgtgtcgct tccagccacc
    15781 gtttgccgtt ttccgatacc agtatggacg ccataatacg tatttacgcg ccgtgtaaag
    15841 cagaagaatt agcacgagta gtgaagcccg gcggctgggt cattactgcc acgccgggac
    15901 cgcgacattt gatggagctg aaggggctga tttacaatga agtacatctt catgcacctc
    15961 atgcagaaca actggaaggt tttacattac agcagagtgc ggagttgtgt tatccgatgc
    16021 gtcttcgcgg tgatgaagcc gtcgcattat tgcagatgac gccgtttgcc tggcgtgcga
    16081 agccagaagt ctggcaaaca ctggcagcaa aagaagtgtt cgactgccag acggacttta
    16141 atattcacct ctggcagcgt tcttattaac cgtggaagtg cgtccagagg atctggacgc
    16201 cgatgccgat cagcaccagc ccgccgagaa tttccgcttt tttcccaata attgagccga
    16261 taaagcgacc aaccatcatc cctaatgtcg acataatcaa ggttgcacaa ccaatggcca
    16321 atgcggtcgt gataatgttg acctgaagga aagcaagacc aacacccaca gccatggcat
    16381 ccaggctggt ggcaatcgcg gtggttacca gtagccagaa accgtgccga cggcgcggct
    16441 cttcatcttc atcatctgcg ccacgaaaac cctcaataat cattcgcccg ccgaggaata
    16501 tcagcagcac aaacgcaatc cagtgattcc attcaaggac aaaccggctg gctaacatgc
    16561 ccattcccca gccgatcagc ggcgtcaggg tttcgacggc accaaaaata aggccggttc
    16621 gcaatgcttc agaaaatttc ggtttatgga ggggggcacc tttaccgatt gatgcagcaa
    16681 atgcatccat cgacatacca aacgcaagaa gaacagtagc agtgatattc atgacaacgt
    16741 cctgaccggg gtatccataa aacacatcat cgcccccagc aaacatgcat acatgaacaa
    16801 caagccgcga tgggttttcg cgttcatgca ggcatagcga ttgatgtgca tatggtctcg
    16861 cctgactgaa agtcttcagt ccgtacgtgc cacgttttgc aacgagtatg ttgacacgta
    16921 catttccgga atctggaaat cggctactcc ccaaatgagg gcgcaacctt aacatatttt
    16981 gagaatataa aacaactgtt ggaatatatt atttcattgc tttattgata acgattttca
    17041 tttatgttta tatatcaatg aaacgttaaa ataaataaca aataagggaa gagataatat
    17101 agcctaagct atgtctgatt attaaaaata cactaaacat agctttggct aaattcataa
    17161 gttattgagt tgatac
//
LOCUS       DAJTQY010000081        17052 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.081, whole genome shotgun sequence.
ACCESSION   DAJTQY010000081 DAJTQY010000000
VERSION     DAJTQY010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17052
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.081"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(<1..213)
                     /locus_tag="OO349_002885"
     CDS             complement(<1..213)
                     /locus_tag="OO349_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HCR5867885.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVD"
     gene            523..2295
                     /gene="aspS"
                     /locus_tag="OO349_002886"
     CDS             523..2295
                     /gene="aspS"
                     /locus_tag="OO349_002886"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HCR5867886.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADGMGALLLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            2413..2865
                     /gene="nudB"
                     /locus_tag="OO349_002887"
     CDS             2413..2865
                     /gene="nudB"
                     /locus_tag="OO349_002887"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HCR5867887.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            2894..3634
                     /locus_tag="OO349_002888"
     CDS             2894..3634
                     /locus_tag="OO349_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR5867888.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            3669..4190
                     /gene="ruvC"
                     /locus_tag="OO349_002889"
     CDS             3669..4190
                     /gene="ruvC"
                     /locus_tag="OO349_002889"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HCR5867889.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGMGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLTRGRLR"
     gene            complement(4192..4794)
                     /locus_tag="OO349_002890"
     CDS             complement(4192..4794)
                     /locus_tag="OO349_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HCR5867890.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGQLLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            4863..4928
                     /gene="yobI"
                     /locus_tag="OO349_002891"
     CDS             4863..4928
                     /gene="yobI"
                     /locus_tag="OO349_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HCR5867891.1"
                     /translation="MYIFITHFFTEYVIFKYLLPI"
     gene            5067..5678
                     /gene="ruvA"
                     /locus_tag="OO349_002892"
     CDS             5067..5678
                     /gene="ruvA"
                     /locus_tag="OO349_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HCR5867892.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDTSSETLIREALRAAL"
     gene            5687..6697
                     /gene="ruvB"
                     /locus_tag="OO349_002893"
     CDS             5687..6697
                     /gene="ruvB"
                     /locus_tag="OO349_002893"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HCR5867893.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGAQEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(6844..7629)
                     /gene="znuB"
                     /locus_tag="OO349_002894"
     CDS             complement(6844..7629)
                     /gene="znuB"
                     /locus_tag="OO349_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HCR5867894.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQQVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(7626..8381)
                     /gene="znuC"
                     /locus_tag="OO349_002895"
     CDS             complement(7626..8381)
                     /gene="znuC"
                     /locus_tag="OO349_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HCR5867895.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNCPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            8460..9392
                     /gene="znuA"
                     /locus_tag="OO349_002896"
     CDS             8460..9392
                     /gene="znuA"
                     /locus_tag="OO349_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HCR5867896.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            9408..10730
                     /gene="mepM"
                     /locus_tag="OO349_002897"
     CDS             9408..10730
                     /gene="mepM"
                     /locus_tag="OO349_002897"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HCR5867897.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            10850..11821
                     /gene="lpxM"
                     /locus_tag="OO349_002898"
                     /gene_synonym="msbB"
     CDS             10850..11821
                     /gene="lpxM"
                     /locus_tag="OO349_002898"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707715.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCR5867898.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVGWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IERRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(11952..13394)
                     /gene="pyk"
                     /locus_tag="OO349_002899"
     CDS             complement(11952..13394)
                     /gene="pyk"
                     /locus_tag="OO349_002899"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HCR5867899.1"
                     /translation="MSKRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(13522..14391)
                     /gene="hexR"
                     /locus_tag="OO349_002900"
                     /gene_synonym="yebK"
     CDS             complement(13522..14391)
                     /gene="hexR"
                     /locus_tag="OO349_002900"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HCR5867900.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            14728..16203
                     /gene="zwf"
                     /locus_tag="OO349_002901"
     CDS             14728..16203
                     /gene="zwf"
                     /locus_tag="OO349_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HCR5867901.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKVGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            16438..17052
                     /locus_tag="OO349_002902"
                     /pseudo
     CDS             16438..17052
                     /locus_tag="OO349_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
BASE COUNT         4352 a         4203 c         4434 g         4063 t
ORIGIN      
        1 atcaaccggc tgttttaatg cttcggcgta atcggcagac cagccaactc gcaccagaaa
       61 cacgggctga ccgctggcgc gaaatttcgc cgccagcttc ccggcgcgat taactacctc
      121 atcggcagta tgtggaccac cggcaaaagg taagatgcct tcttgtaaat cgatcaccac
      181 caacgcagtg gttttagcat taagttcaag catgctgact cctgaaaaaa caaaatcgtg
      241 cctcacacct taatgcgatt agccgatggc ggaacgacaa attttgttaa tttttatgag
      301 ttcgggcaac acctttatag caaactggcg tagagtctga tttcaggccc gatataaagt
      361 ggtaacgagg cgttccagcg cgggccaggc tcttacggtg cggcagaatt tccagtataa
      421 tagccgcctt ttttcattca gttgtgacat acagctaacg ctgcgacttg gtcacctgcg
      481 cagcaggggg catccgccgg cagctaagtt aagggatatc tcatgcgtac agaatattgt
      541 ggacagctcc gtttgtccca cgtggggcag caggtgactc tgtgtggttg ggtcaaccgt
      601 cgtcgtgatc ttggtagcct gatcttcatc gatatgcgcg accgcgaagg tatcgtgcag
      661 gtatttttcg atccggatcg tgcggacgcg ttaaagctgg cctctgaact gcgtaatgag
      721 ttctgcattc aggttacggg caccgtacgt gcgcgtgacg aaaaaaatat taaccgcgat
      781 atggcgaccg gcgaaattga agtgctggcg tcctcgctga ctatcatcaa ccgcgcagat
      841 gttctgccgc ttgactctaa ccacgtcaac accgaagaag cgcgtctgaa ataccgctac
      901 ctcgacctgc gtcgcccgga aatggcgcag cgcctgaaaa cccgcgctaa aatcaccagc
      961 ctggtgcgcc gttttatgga tgaccacggc ttcctcgaca tcgaaactcc gatgctgacc
     1021 aaagccacgc cggaaggcgc gcgtgactac ctggtgcctt ctcgtgtgca caaaggtaaa
     1081 ttctacgcac tgccgcaatc cccacagttg ttcaaacagc tgctgatgat gtccggtttt
     1141 gaccgttact atcagatcgt taaatgcttc cgtgacgaag acctgcgcgc tgatcgtcag
     1201 cctgaattta ctcagatcga tgtggaaact tctttcatga ccgcgccgca agtgcgtgaa
     1261 gtgatggaag cgctggtgcg tcatttgtgg ctggaagtga agggcgtgga tctgggcgat
     1321 ttcccggtaa tgacctttgc ggaagcagaa cgccgttatg gttctgataa accggatctg
     1381 cgtaacccga tggaactgac cgacgttgcc gatctgctga aatctgttga gtttgcagta
     1441 tttgcggggc cggcgaacga tccgaaaggc cgtgttgccg ctctacgtgt tccgggtggc
     1501 gcatcgctga cccgtaagca gatcgacgaa tacggtaact tcgttaaaat ctacggcgcg
     1561 aaaggtctgg cttacatcaa agttaacgaa cgcgcgaaag gtctggaagg tatcaacagc
     1621 ccggtagcga agttccttaa tgcagaaatc atcgaagcca tcctggatcg tactgccgcg
     1681 caagatggcg atatgatttt cttcggtgcc gacaacaaga aaattgttgc cgacgggatg
     1741 ggcgcactgc tcctgaaagt gggtaaagac cttggtctga ccgacgaaag caaatgggca
     1801 ccgctgtggg ttatcgactt cccgatgttt gaagacgacg gtgaaggcgg cctgacggca
     1861 atgcaccatc cgttcacctc accgaaagat atgactgctg cagaactgaa agctgcaccg
     1921 gaaaatgcgg tggcgaacgc ttacgatatg gtcatcaatg gttacgaagt gggcggtggt
     1981 tcagtacgta tccataatgg tgatatgcag cagacggtgt ttggtattct gggtatcaac
     2041 gaagaggaac agcgcgagaa attcggcttc ctgctcgacg ccctgaaata cggtactccg
     2101 ccgcacgcag gtctggcatt cggtcttgac cgtctgacca tgctgctgac tggcaccgac
     2161 aatatccgtg acgttatcgc cttcccgaaa accacggcgg cagcgtgtct gatgactgaa
     2221 gcaccgagct ttgctaaccc gactgcactg gctgagctga gcattcaggt tgtgaagaag
     2281 gctgagaata actgatatga ctcaaataca cgaaatcatt cgcgttgcat caaggcggca
     2341 actgagtgaa ctcccatgag catagataac tatgtgaatg ggatgagcga aggcagtcaa
     2401 cgaagaggca gcgtgaagga taaagtgtat aagcgtcccg tttcgatctt agtggtcatc
     2461 tatgcacaag atacgaaacg ggtgctgatg ttgcagcggc gtgacgatcc cgatttctgg
     2521 cagtcggtaa ccggcagcgt ggaagagggt gaaaccgcgc cgcaagctgc catgcgcgaa
     2581 gtaaaggaag aggttaccat tgatgttgtc gctgaacaac tgaccttaat tgactgtcag
     2641 cgcacggtag agtttgaaat tttttcacat ttacgtcatc gctatgcgcc gggcgtgacg
     2701 cgtaatacgg aatcatggtt ctgtcttgcg cttccgcacg agcggcagat cgttttcact
     2761 gaacatctgg cttacaagtg gcttgatgcg cctgctgcgg cggcgctcac taagtcctgg
     2821 agcaaccggc aggcgattga acagtttgta attaacgctg cctgaacagg cagggctatc
     2881 tggagatatt tttatggcag gtcatagtaa atgggccaac accagacatc gtaaagctgc
     2941 gcaggatgct aagcgcggta aaatcttcac taaaatcatt cgtgagctgg taaccgcggc
     3001 taagctgggc ggtggcgatc cggacgctaa cccgcgtctg cgtgcggcgg tggataaagc
     3061 actgtctaac aacatgaccc gtgacacact gaaccgcgca attgcacgtg gtgtgggcgg
     3121 tgatgatgat gcaaacatgg aaaccatcat ctacgaaggt tacggtcctg gcggcacggc
     3181 aatcatgatt gaatgtctgt ctgacaaccg caaccgtacc gttgctgaag tgcgtcacgc
     3241 attcagcaaa tgtggcggta acctcggcac tgacggttcc gtagcttatc tgttcagcaa
     3301 aaaaggcgtg atctccttcg agaaaggcga cgaagacacc atcatggaag cagctctgga
     3361 agcaggtgct gaagacgttg tgacatatga tgacggcgcg attgatgtct acactgcatg
     3421 ggaagaaatg ggtaaagtgc gcgacgctct ggaagcggca ggtctgaaag cagacagcgc
     3481 ggaagtttcc atgatcccgt ccaccaaagc tgatatggat gcagaaaccg cgccgaaact
     3541 gatgcgtctg atcgatatgc tggaagattg cgacgacgtg caggaagttt accataacgg
     3601 tgaaatctct gatgaggttg cagcgactct ctgatgatgc ctgctaaaca gcaaaacgga
     3661 gacgcgtgat ggctattatt ctcggcattg acccgggttc gcgcgtgacc ggctacggcg
     3721 tcatccgcca ggtaggtagg caactgtcct acctgggtag cggatgcata cgcaccaaag
     3781 tggatgattt accgtctcgt ctgaagctca tctatgcggg cgtgacggaa atcatcaccc
     3841 agttccagcc tgattatttc gccattgaac aagtctttat ggcgaagaac gctgactcag
     3901 ccctgaaact gggccaggcg cgcggcgtgg cgattgttgc ggcggtgaat caggaattgc
     3961 cggtatttga atacgcggca cgtcaggtaa agcaaacggt ggtaggtatg ggtagtgccg
     4021 aaaaaagcca ggtgcagcat atggtccgca ccttgctgaa actgcccgct aatccacagg
     4081 cggatgccgc cgatgcgctg gcgattgcca tcacccactg ccacgttagt cagaatgcga
     4141 tgcagatgag cgaatcgcgg ctgaacctga cgagagggcg actgcgttaa gttataccgc
     4201 ctcggtcagt tccggctgag gcgtttcaac tccctccgcg tgaatcaaca ccaaacccgg
     4261 atgatgccac aaactggctg gcgtgacggt tttacgctcc cacggaatag aacctaagaa
     4321 ccagaatttc cagaaagtga gttttgcatt cggattgcta tttaacagtt gtccaaacgt
     4381 ctctatttca cccaccggaa tacatagcgc ctctttataa atatcaaaga caaattttga
     4441 acagaactgg cgcgaagatt cgtatttaaa acccgtgtgg taaagtttgc gtagccggga
     4501 gggaacctgt tcaacaattc gttgtttttg ttgttctgtc agcccagcgt ctaatcgctt
     4561 tatagcatag cgttgattag acgaacgttt aataaaacga gatagcgtgg tgatggttga
     4621 aaggggaacc cggctttctg caaccaggaa gtcttcaccg ttatgaccga taatgatccc
     4681 gacgtgatta ctccagcaat ttgatgcagc tgatatttga ccaaataagg cagcacctat
     4741 acatgtaaag acgatatctc caatttcata ttcagcaggg taattaatat tcacgttgtc
     4801 attccattga aatagatacg aatatacgta atagcataaa aaattatttt tcaggagtcg
     4861 caatgtatat ttttattacg catttcttca ctgaatatgt aatattcaaa tatttgcttc
     4921 caatataacc tgttgaataa attatactgt gccatttttc agatcatcgg gacacctcgc
     4981 aagttttctt catccttcgc tggatatcta tccagcattt ttttatcata cagcattatt
     5041 tttgattcat tacgcaggag cgtcatgtga taggcagact cagaggcatc atcattgaaa
     5101 aacaaccccc gctggtgtta attgaagtgg gcggcgtagg ctatgaagtg catatgccga
     5161 tgacctgttt ttatgaactc cctgaagcgg gtcaggaagc gatcgttttc acccactttg
     5221 tggtgcgtga agacgcgcaa ctgctgtacg gttttaacaa taaacaagaa cgcacgttgt
     5281 tcaaagagtt gatcaaaacc aacggcgtcg gcccgaagtt ggccctggcg atcctctccg
     5341 gaatgtcagc gcagcagttc gttaatgccg ttgagcgtga agaagtgggg gcactggtga
     5401 aactgccggg tattggcaaa aaaaccgccg aacgcttgat tgttgaaatg aaagaccgat
     5461 ttaaaggttt gcatggcgat ctctttacgc cagccgccga cctggtactc acgtctcctg
     5521 ctagcccggc gaccgacgat gctgaacaag aagcagttgc cgcgctggtg gcgctaggct
     5581 ataaaccaca agaagcaagc cgcatggtga gcaaaatcgc tcgccctgac accagcagtg
     5641 aaacattaat tcgcgaagcc ctacgcgccg cgttatgagg taaaggatga ttgaagcaga
     5701 ccgtctgatt tctgccggta ccactttgcc ggaagatgta gcagatcgcg ccattcgccc
     5761 taaattactg gaagagtatg ttggtcagcc gcaggttcgt tcacagatgg agattttcat
     5821 caaagcagcg aaactgcgcg gcgatgccct cgatcatttg ttgatttttg gtcctccggg
     5881 gttgggtaaa actacgcttg ccaacattgt cgccaatgaa atgggcgtta atttacgcac
     5941 gacttctggt ccggtgctgg aaaaggcggg cgatttggct gcgatgctca ctaaccttga
     6001 accgcatgac gtgctgttta ttgatgagat ccaccgttta tcgccagttg ttgaagaagt
     6061 gctgtacccg gcaatggaag actaccaact ggatatcatg attggtgaag gtccggcggc
     6121 acgctccatt aaaattgatt tgccgccgtt taccctgatt ggcgcaacca cgcgcgcagg
     6181 ttcgctgaca tcaccgttgc gcgaccgttt tggtattgtg caacgtctgg agttttatca
     6241 ggtgccggat ctgcaatata tcgtcagtcg cagtgcacgc tttatggggc ttgagatgag
     6301 tgatgacggt gcgcaggaag tagctcgtcg cgctcgcggt acgccacgta ttgccaaccg
     6361 tctgctgcgt cgagtgcgtg atttcgccga agtgaagcac gatggcacca tctcggcaga
     6421 tatcgctgct caggcgctgg atatgttgaa tgtcgatgct gaaggtttcg attatatgga
     6481 ccgcaaattg ttgctggcgg taatcgataa gttctttggt gggccggtag gtctggataa
     6541 cctggcggca gccattggcg aagaacgtga aaccattgaa gatgtactgg aaccttattt
     6601 gattcagcaa ggctttttgc agcgtacacc gcgcgggcgt atggcgacga cgcgggcgtg
     6661 gaatcacttt ggcataacgc cgccagaaat gccgtaagtc ggattggtcg ctcgcgccgc
     6721 atccgacact ggcagtgtgt ctgatgcgac actgttcgcg tcttatcagg ccagccagag
     6781 atagcgaacc gtaggtcgga taaggcgctc gcgccgcatc cgacaaatgt gttcagcgat
     6841 agattagctg gcctgctttt tcatcatact gataataaat aacagtgccg cacatagcac
     6901 caccgacgga cccgccggcg tatcgtaaaa cgcggaaaag gttaaaccgc cagttactgc
     6961 caccatcccc accaaaacag cgacaccagc catctgttcc ggcgtacggg caaagcgacg
     7021 cgcagtagct gcaggaataa tcagcaacga agtaataatc aacgcaccga caaatttcat
     7081 cgctacacca atcgtcaatg ccgtcaccag catcaacaac aatttcactt gctgtaattt
     7141 cacaccatca acaaacgcca gatccgggct aatcgtcatc gacagcaaat tgcgccattg
     7201 ccagaacaaa atagccacca cgatgaccac gccaatcgca atagagatga gatcttctgg
     7261 cgtcactgcc agtaaatcac cgaacaggta agccatcaaa tcaacacgaa tgttagacat
     7321 cagactaacg accactaggc ccagcgacag ggcactgtgc gccataatcc ctaataacgt
     7381 gtcgatcgcc agctgtggac gcttctccag ccataccaga ccgcccgcca gcagcagcgt
     7441 aacggcaatc accgcataga atggattcac gtccagcaac aaaccaaacg cgacaccaag
     7501 taatgaggca tgagccagcg tatcaccgaa ataagacata cgacgccaga ctacaaacga
     7561 acccagcgga cccgcggcac aggcgagcat gatcccggct aaccaaccgg gaaataataa
     7621 ttcaatcatg agcgatcatt tccccgacgc aaaacaattc gtccctgtaa atcgtgacga
     7681 tgattatgat gatggcgata gatacccagt tgttcagcac cacgaggacc aaacattgaa
     7741 ataaactccg gatgcaggga aacaacttcc ggtgtgccgg aacaacaaat gtggtgattc
     7801 aggcacagca cttcatcggt ttttgccatc accagatgca gatcgtgaga aaccattaaa
     7861 acgccacaat ccagttcacg acgcaactgg tcaataaggt catataacgc cacttggcca
     7921 tttacatcca cgccttgagt gggttcatcc agcactaata attgcgggca atttaacaat
     7981 gctcgcgcta atagtacacg ctgcgtttcg ccaccagaga gcttttgcat cggtgcgtta
     8041 atcaggtgcc cggcctggac acgtttcagt gcaggcaaaa tatcttcttt atgtgtacca
     8101 gggcgtaagc gtaaaaaacg gtttacggtc agtggcaacg tggtgtcgag atacagcttc
     8161 tgcggtacat agccgatgcg cagttttccg ttgcgcttga taaccccttc atcgggtgtt
     8221 accagcccga gcactacccg taccagtgtc gacttacctg cgccatttgg cccaagtaaa
     8281 gtcaaaattt ttccaggttt aagttccagc gacacatcag agaggacgcg gcgttggcca
     8341 aaagaaaccg agacattttc cagggaaacc agacttgtca tgttaatttt agtcttgcag
     8401 tagtcatgaa atgttataat atcacacttc tcatattcat tacgatgatt ggtcgcatta
     8461 tgttacataa aaaaacgctt cttttcgcag cattatccgc cgctctctgg ggaggtgcaa
     8521 cacaggccgc agatgccgcc gttgtcgctt cgcttaaacc cgttgggttc atcgcttctg
     8581 ccattgctga tggtgtaaca gaaacggagg ttttactgcc tgacggcgct tcagaacatg
     8641 attattcact gcgtccatcg gatgtaaaac gcttacagaa cgcggactta gtcgtttggg
     8701 ttggcccgga gatggaagcg tttatgcaaa aaccggtaag caaattacca gaagcgaagc
     8761 aggtaacgat tgcgcagctt gaagatgtga aaccgctact gatgaaaagt attcacggcg
     8821 atgatgatga tcacgaccac gcggaaaaaa gtgacgaaga tcaccatcac ggcgatttca
     8881 acatgcatct ttggctttcc ccagagatag cgcgggctac agcggttgca atccatggaa
     8941 aattagtgga acttatgccg caaagtcgag ccaaacttga cgccaacctg aaggattttg
     9001 aggcacaatt ggcctcaacc gaaacgcagg ttggtaacga gctcgcgccg ctcaagggga
     9061 aaggttattt cgtttttcac gatgcttacg gctatttcga aaaacagttc ggactgacac
     9121 cgcttggtca ttttaccgtt aaccctgaga ttcaacctgg cgcgcagcgt ttacatgaaa
     9181 taagaacaca gttggttgag caaaaagcaa cctgcgtttt tgctgagcca cagttcaggc
     9241 cagcggtcgt tgagagcgtc gcacgaggga catccgttcg tatgggaacg ttggatcccc
     9301 tggggacgaa tatcaaactg ggtaaaacaa gctattcaga attcctgagt caattagcca
     9361 accagtatgc gagctgcctg aaaggagatt aatgaggaag tgattacgtg caacagatag
     9421 cccgctctgt cgccttggcg tttaataatt taccgcgacc acaccgcgtt atgttggggt
     9481 cgctcaccgt tcttactctg gccgtcgctg tctggcggcc ctatgtttat caccgtgatg
     9541 ccacgccaat tgtcaaaacc attgagctgg aacagaacga aattcgttcg ctcttacctg
     9601 aagccagtga gccgattgat caagctgcac aagaagatga agccattccc caggacgaac
     9661 tggatgacaa aatcgccggt gaagcgggcg tgcatgaata tgttgtttcc actggcgata
     9721 cgctaagcag cattctcaat cagtatggta ttgatatggg tgatatcacc caacttgctg
     9781 ctgccgacaa agaattgcgt aacctgaaaa tcggtcaaca actctcctgg acattaaccg
     9841 cggacggcga actgcagcgc ctcacctggg aagtgtctcg tcgtgaaacc cgaacctatg
     9901 accgtactgc cgctaacggt tttaaaatga ccagcgaaat gcagcaagga gagtgggtta
     9961 acaatctgct gaaaggtacc gtcggaggga gctttgttgc cagcgccaga aacgccggtt
    10021 taaccagcgc cgaagtgagc gcagtgatta aagccatgca gtggcaaatg gatttccgca
    10081 aactgaaaaa aggcgatgaa tttgcggtat taatgtctcg agaaatgctt gatggtaaac
    10141 gtgagcaaag ccagctgctg ggcgtacgtt tgcgttcaga aggtaaagat tattacgcaa
    10201 tccgcgctga ggatggcaaa ttctacgatc gtaacggtac tggtctggcg aaaggattct
    10261 tgcgattccc gacggcgaaa cagttccgta tctcgtctaa ctttaacccg cgtcgaacta
    10321 atccggtgac aggtcgcgtt gcaccacaca gaggtgttga tttcgccatg ccacaaggta
    10381 cgccagtgct ttcagtgggt gacggtgaag tggtggttgc caaacgcagt ggcgcagcag
    10441 gttattatgt ggctattcgt catggtcgca gctacaccac gcgttatatg cacttgcgca
    10501 agattctggt gaaaccggga cagaaggtga aacgtggcga ccgtatcgcg ctttccggta
    10561 ataccggacg ttcaactggg ccgcatctgc actatgaagt atggataaac cagcaggccg
    10621 taaacccgct gacggcaaaa ctgccgcgta ccgaagggct gaccggctcc gatcgtcgcg
    10681 aattcctggc gcaggccaaa gagattgtgc cgcagctacg gtttgattaa ttaacatcca
    10741 ttcgcagccg gtacgcagtc agtaccggct ttttttattt ggtgcggggc aagttgtgcc
    10801 gctacactat caccagattg atttttgcct tatccgaaac tggaaaagca tggaaacgaa
    10861 aaaaaataat agcgaataca ttcctgagtt tgataaatcc tttcgccacc cgcgctactg
    10921 gggagcatgg ctgggtgtag cagcgatggc gggtatcgct ttaacgccgc caaagttccg
    10981 tgatcccatt ctggcacggc tgggacgttt tgccggacga ctgggaaaaa gctcacgccg
    11041 tcgtgcgtta atcaatctgt cgctctgctt tccagaacgt agtgaagctg aacgcgaagc
    11101 gattgttgat gagatgtttg ccaccgcgcc gcaagcgatg gcaatgatgg ctgagttggc
    11161 aatacgcggg ccggagaaaa ttcagccgcg cgttggctgg caagggctgg agatcatcga
    11221 agagatgcgg cgtaataacg agaaagttat ctttctggtg ccgcacggtt gggccgtcga
    11281 tattcctgcc atgctgatgg cctcgcaagg gcagaaaatg gcagcgatgt tccataatca
    11341 gggcaacccg gtttttgatt atgtctggaa cacggtgcgt cgtcgctttg gcggtcgtct
    11401 gcatgcgaga aatgacggta ttaaaccatt catccagtcg gtacgtcagg ggtactgggg
    11461 atattattta cccgatcagg atcatggccc agagcacagc gaatttgtgg atttctttgc
    11521 cacctataaa gcgacgttgc ccgcgattgg tcgtttgatg aaagtatgcc gtgcgcgcgt
    11581 tgtaccgctg tttccgattt atgatggcaa gacgcatcgt ctgacgattc aggtgcgccc
    11641 accgatggat gatctgttag aggcggatga tcatacgatt gagcggcgga tgaatgaaga
    11701 agtcgagatt tttgttggtc cgcgaccaga acaatacacc tggatactaa aattgctgaa
    11761 aactcgcaaa ccgggcgaaa tccagccgta taagcgcaaa gatctttatc ccatcaaata
    11821 aaaaagcctc tcgcgaggag aggccttcgc ctgatgataa gttcaagttt gcttcagaat
    11881 attcgaaatc tgttgaacta tcattgaact ataggccgga tgtggcgttt tcgccgcatc
    11941 cggcaacgta cttactctac cgttaaaata cgcgtggtat tagtagaacc cacggtactc
    12001 atcacgtcgc cttgggtgac aatcaccagg tcaccagaca tcaagtaacc tttatcgcgc
    12061 agcagattaa ccgcttcgct ggcagctgct acgccgtcat tagcgctatc aaagtgcacc
    12121 ggcgtaacgc cacgatagag agcagtcagg ttcagcgtac gttcatggcg cgacatggcg
    12181 aaaattggta gaccagagct gatacgggag gtcatcagcg cggtacgacc cgattcggtc
    12241 atggtaatga tcgccgtaac gcccttcagg tggttagctg cgtacattgc cgacatggca
    12301 atagcttctt ccacattgtc gaactgaacg tccagacggt gtttagaaac gttgatgctc
    12361 gggatttttt ccgcacccag gcaaacgcgc gccatggctg caacagtttc tgacggatac
    12421 tgcccagcgg cagtttctgc agacagcatc acagcgtcag taccatccag aacggcgttt
    12481 gctacgtcca tgacttctgc acgcgtcggc atcgggttag taatcattga ctccatcatc
    12541 tgggtcgccg tgattaccgc tcggtttagc tgacgcgcac gacggatcaa cgctttctga
    12601 atgccgacca gttccgggtc gccaatttcc acaccgaggt cgccacgtgc aaccattacc
    12661 acgtcagagg cgaggatgat gtcatccatt gcatcctggc tgcaaacggc ttccgcacgt
    12721 tcaaccttgg caacaatttt cgcatcacat cctgcatcgc gtgccaggcg acgggcatag
    12781 ttcagatctt cgccacagcg tgggaaggag acagccaggt aatctacgcc aatcaacgcc
    12841 gcagtcttaa tgtctgcttt gtctttttcg gtcagcgctt cagccgacaa accgccgcca
    12901 agtttgttga tacctttatt gttggagagg ggaccaccga cggtgacttc ggtgaacact
    12961 ttcatgccct gaacttccag tacttttaac tggacgcgac catcgtccag cagcaggatg
    13021 tcaccaggca cgacgtcagc aggcaggcct ttgtagtcga taccgacttt ttctttgtcg
    13081 ccttcacctt tacccaggtt ggcgtcgagc aggaatttat ccccaatatt gaggaaaact
    13141 ttgccttctt taaaggtgga tacacggatt ttgggcccct ggaggtcacc cagaatagcc
    13201 acatgacgcc ccagttttgc ggcaatctca cgaactttat ccgcgcgcat tttgtgatct
    13261 tcaggcgagc cgtgagaaaa gttcatacgt acaacgttgg cacccgccgc gataactttt
    13321 tcaagattat tatcgcgatc tgttgctggg cctaacgtgg taacgatttt tgttctgcga
    13381 agccttttgg acatgtaata ctccgttgac tgaaacaacc aggtgttgct tgaacatgaa
    13441 atccgaatga aataacgccg cgatggcgat aaattaatct cattcggatg tcgtataaga
    13501 ttaggacagt gacagtcgct tttagcgatc gtcacttaaa ttaagtaact gcttatcaaa
    13561 acgcgattcc ttcagcgctt ccttgacccg cttcaagtta tctctgaatt ttgcaccgcg
    13621 tcgcaaagta aatcctgtcg ccagcacatc tatcacggtc agctgtgcaa gtcgagaaac
    13681 catgggcata taaatgtcag tatcttccgg tacgtcgagg gtaattgcca gcgttgcttc
    13741 ccgggcgagc ggggtacccg cagaggtgag ggcaatcacc atggcgtcgt tttcgcgtgc
    13801 cagctgcgcc agctcgacca gattttttgt tcttcctgtg tgagaaatca gcaccaccac
    13861 gtctccgtcg ctacaattca tacaactcat gcgttgcagc acgatatcat cggagtacac
    13921 caccggaaca ttaaaacgaa agaacttatt catcgcatcg tgggcaacgg cggcagaaga
    13981 gcctaatccg aaaaaggcga ttttttttgc ctgagtgagc aagtcgacgg cgcggttgat
    14041 ggcagattta tccagtgaat gacggacatg atcgagcgtt gccattgcgg actcaaatat
    14101 tttccccgtg tatgattcaa cgctgtcatc ttcattgacg ttgcgattaa catagggagt
    14161 gccattcgcc agactttgtg ccagatgaag tttaaaatca ggaaaaccgc gcgtgtccat
    14221 gctgcgacag aaacgattca ccgtcggttc gctaacattg gcttccagcg ccagagcagc
    14281 aatactcgaa tggatcgcgt tatcgggcga agccagaatg acctcggcaa ctttgcgctc
    14341 tgatttgctc aaatgttcca gctgagactg gattttttcc agcatattca tgatgtaaag
    14401 agactcacgg gtaatgacga tttccgcact gaaagaaatc gaaatgcagt tttgtcagat
    14461 attacgcctg tgtgccgtgt taatgacaaa agcagataaa aaagttgtta tttttttcat
    14521 aacatgatca gtgtcagatt tttaccgaat ggaaaacgat gattttttta tcagttttgc
    14581 cgcactttgc gcgcttttcc cgtaatcgca cgggtggata agcgtttaca gttttcgcaa
    14641 gctcgtaaaa gcagtacagt gcaccgtaag aaaattacaa gtataccctg gcttaagtac
    14701 cgggttagtt aacttaagga gaatgacatg gcggtaacgc aaacagccca ggcctgtgac
    14761 ctggtcattt tcggcgcgaa aggcgacctt gcgcgtcgta aattgctgcc ttccctgtat
    14821 caactggaaa aagtcggtca gctcaacccg gacacccgga ttatcggcgt agggcgtgct
    14881 gactgggata aagcggcata taccaaagtt gtccgcgagg cgctcgaaac tttcatgaaa
    14941 gaaaccattg atgaaggttt atgggacacc ctgagtgcac gtctggattt ttgtaatctc
    15001 gatgtcaatg acactgctgc attcaaccgt ctcggcgcga tgctggatca aaaaaatcgt
    15061 atcactatta actactttgc catgccgccc agcacttttg gcgcaatttg caaagggctt
    15121 ggcgaggcaa aactgaatgc taaaccggca cgcgtagtca tggagaaacc gctggggacg
    15181 tcgctggcga cctcgcagga aatcaatgat caggttggcg aatacttcga ggagtgccag
    15241 gtttaccgta tcgaccacta tcttggtaaa gaaacggtgc tgaacctgtt ggcgctgcgt
    15301 tttgctaact ccctgtttgt gaataactgg gacaatcgca ccattgatca tgttgagatt
    15361 accgtggcag aagaagtggg gatcgaaggg cgctggggct attttgataa agccggtcag
    15421 atgcgtgaca tgattcagaa ccacctgcta caaattctct gcatgattgc gatgtctccg
    15481 ccatctgacc tgagcgcaga cagcatccgc gatgaaaaag tgaaagtact gaagtccctg
    15541 cgccgcatcg accgctccaa cgtacgcgaa aaaaccgtac gcgggcaata tactgcgggc
    15601 ttcgcccagg gcaaaaaagt gccgggatat ctggaagaag agggcgcgaa caaaagcagc
    15661 aatacagaaa ccttcgtggc gatccgcgtc gacattgata actggcgctg ggccggtgtg
    15721 ccattctacc tgcgtactgg taaacgtctg ccgaccaaat gttctgaagt cgtggtctat
    15781 ttcaaaacac ctgaactgaa tctgtttaaa gagtcgtggc aggatctgcc gcagaataaa
    15841 ctgactatcc gtctgcaacc tgatgaaggc gtggatatcc aggtactgaa taaagttcct
    15901 ggccttgacc acaaacataa cctgcaaatc accaagctgg atctgagcta ttcagaaacc
    15961 tttaatcaga cgcatctggc ggatgcctat gaacgtttgc tgctggaaac catgcgtggt
    16021 attcaggcac tgtttgtacg tcgcgacgaa gtggaagaag cctggaaatg ggtagactcc
    16081 attactgagg cgtgggcgat ggacaatgat gcgccgaaac cgtatcaggc cggaacctgg
    16141 ggacccgttg cctcggtggc gatgattacc cgtgatggtc gttcctggaa tgagtttgag
    16201 taatatcagc gcttatcctt tatggttatt ttaccggtaa catgatcttg cacagattgt
    16261 agaacaattt ttacactttc aggcctcgtg cggattcacc cacgaggctt tttttattac
    16321 actgactgaa acgtttttgc cctatgagct ccggttacag gcgtttcact cataaatcct
    16381 ctgaatgaaa cgcgttgtga atcatcctgc tctgacaact caatttcagg agcctttatg
    16441 aatccacaat tgttacgcgt aacaaattga atcattgaac gttcgcgcga gactcgctct
    16501 gcttatctcg cccggataga acaagcgaaa acgtcgaccg ttcatcgttc gcagttggca
    16561 tgcggtaacc tggcacacgg tttcgctgcc tgccagccag aagacaaagc ctctttgaaa
    16621 agcatgttgc gtaacaatat cgccatcatc acctcctata acgacatgct ctccgcgcac
    16681 cagccttatg aacactatcc agaaatcatt cgtaaagccc tgcatgaagc gaatgcggtt
    16741 ggtcaggttg cgggcggtgt tccggcgatg tgtgatggtg tcacccaggg gcaggatgga
    16801 atggaattgt cgctgctaag ccgcgaagtg atagcgatgt ctgcggcggt ggggctgtcc
    16861 cataacatgt ttgatggtgc tctgttcctc ggtgtgtgcg acaagattgt cccgggtctg
    16921 acgatggcag ccctgtcgtt tggtcatttg cctgcggtgt ttgtgccgtc tggaccgatg
    16981 gcaagcggtt tgccaaataa agaaaaagtg cgtattcgcc agctttatgc cgaaggtaaa
    17041 gtggaccgca tg
//
LOCUS       DAJTQY010000082        17006 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.082, whole genome shotgun sequence.
ACCESSION   DAJTQY010000082 DAJTQY010000000
VERSION     DAJTQY010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17006
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.082"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(175..1071)
                     /gene="yhaJ"
                     /locus_tag="OO349_002903"
     CDS             complement(175..1071)
                     /gene="yhaJ"
                     /locus_tag="OO349_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HCR5867902.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFAGK"
     gene            1176..1877
                     /locus_tag="OO349_002904"
     CDS             1176..1877
                     /locus_tag="OO349_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HCR5867903.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDTCPQRENPLIQKLALNMDKQQLIASPEGAMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            1900..2064
                     /locus_tag="OO349_002905"
     CDS             1900..2064
                     /locus_tag="OO349_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5867904.1"
                     /translation="MSKKSTKKRQPAKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(2276..3586)
                     /locus_tag="OO349_002906"
     CDS             complement(2276..3586)
                     /locus_tag="OO349_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708914.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HCR5867905.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTHQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITAIMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(3614..4945)
                     /locus_tag="OO349_002907"
     CDS             complement(3614..4945)
                     /locus_tag="OO349_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HCR5867906.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTDMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSLFLAFS"
     gene            complement(5220..6584)
                     /gene="tdcG"
                     /locus_tag="OO349_002908"
     CDS             complement(5220..6584)
                     /gene="tdcG"
                     /locus_tag="OO349_002908"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCR5867907.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPCRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(6656..7045)
                     /locus_tag="OO349_002909"
     CDS             complement(6656..7045)
                     /locus_tag="OO349_002909"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HCR5867908.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(7059..9353)
                     /gene="tdcE"
                     /locus_tag="OO349_002910"
     CDS             complement(7059..9353)
                     /gene="tdcE"
                     /locus_tag="OO349_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HCR5867909.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKSEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(9387..10595)
                     /gene="tdcD"
                     /locus_tag="OO349_002911"
     CDS             complement(9387..10595)
                     /gene="tdcD"
                     /locus_tag="OO349_002911"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HCR5867910.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENAHVICVVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(10621..11952)
                     /gene="tdcC"
                     /locus_tag="OO349_002912"
     CDS             complement(10621..11952)
                     /gene="tdcC"
                     /locus_tag="OO349_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HCR5867911.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTLSMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(11974..12963)
                     /gene="tdcB"
                     /locus_tag="OO349_002913"
     CDS             complement(11974..12963)
                     /gene="tdcB"
                     /locus_tag="OO349_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HCR5867912.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(13062..14000)
                     /gene="tdcA"
                     /locus_tag="OO349_002914"
     CDS             complement(13062..14000)
                     /gene="tdcA"
                     /locus_tag="OO349_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HCR5867913.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
     gene            14190..14534
                     /gene="tdcR"
                     /locus_tag="OO349_002915"
     CDS             14190..14534
                     /gene="tdcR"
                     /locus_tag="OO349_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HCR5867914.1"
                     /translation="MTGITIFYGDNIIRYVVNTKKGLRPYFKQLPDNYQAKFELNLMS
                     KFSNFIINKPFSAINTAARHIFSRYLLENKYLFYQYFKISNTGIDHLEQLINVNFFSS
                     DRTSFCECNRFP"
     gene            14790..15329
                     /locus_tag="OO349_002916"
     CDS             14790..15329
                     /locus_tag="OO349_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5867915.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTVKRKFAEIVSTN
                     DVNKIYSISNELRRVLSAITALNFYQGDVPSVMIRIQPENMSPFIIDISTGEHDDYII
                     QTLDVGTFAPFGEQCTCSAVNKKELECIKETISKYCAKFTQKEAILTPPRHFNKTCIT
                     SDCWQILFFSPDHFNNDFY"
     gene            15351..15698
                     /locus_tag="OO349_002917"
     CDS             15351..15698
                     /locus_tag="OO349_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5867916.1"
                     /translation="MFPVSSIGNDISSDLVRRKMNDLPESPIGNNLEALAPGIEKLKQ
                     TSIQMVTLLNALQPSGKCIITGDFQKELAYLQNVILYNDSSLRLDFLGYNTQIIQRSD
                     NTCELTINEPLKN"
     gene            15867..16517
                     /locus_tag="OO349_002918"
     CDS             15867..16517
                     /locus_tag="OO349_002918"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5867917.1"
                     /translation="MANTILNHFKLDSTNFTNANMFQVNFVSSTQNATISWDYLLKIT
                     PVLISISDMYSEEKIKFVESCLNELGDITEEQLKIMRFAIIKSIPRATLTDKLENELT
                     KEIYKSSSKINNCLNRIKLPEMKEFSSKKIYDYIDTIIEDYENIKENAYLVVPQINYT
                     MDLNIEDSSSEELIPSRKTKILRTMALRSGNITHMKHITQRINILPERTIRIITTF"
BASE COUNT         4683 a         4339 c         3788 g         4196 t
ORIGIN      
        1 tgttgacaaa gtgcgctttg tttatgccgg atgcggcgta aacgccttat ccagcctaca
       61 aattgccaaa attcaatata ttgcagtgac tgcgtaggcc tgataagcgt agcgcatcag
      121 gcagttttgc gtttgtcagc agtcagaagc accttgtgaa aggtgcttaa tagattattt
      181 tccagcgaac aactttggaa tttcccgcag acaccaggat ttcgcttccc ccatactgtc
      241 acgacgccag gccataataa tatcgatctc gctggtcgat tccgggctga cgacacgcaa
      301 ccgcccttcc gcaatatctt tttcgaccat cggatacggc atcgtcgcca cgccaagccc
      361 cgccagtaat gcctgacgtt tatcttcaat cgtgctcacc gttaagcgcg gctgtttgtc
      421 cagcagctgt acggtcaaca ccgggcgctc acgagcggta tccgctaccg caattccacg
      481 atatttcaca cgcgtcactt cagataacgg ttccggctcc tgatgaatcg ggtgatcagg
      541 cgcggcaacg tagacgttca ttaacgtata gagcttgcgc gagttgatct ccgacgagga
      601 acgaaaatgc atatccggcg cgataacgat atccgcccgc ccctgctcca gccgttccca
      661 cgcccccgcc agcacttctg tgatgattgc cagttgggta ttggcttttg ccgccagttt
      721 gtcgattaac gggaaaaagg caggtgtcgg taccagcgct tcggtcacaa tggtgagatg
      781 cgtttcccaa ccgcgcgcga gagcttccgc atcagtagtc agtttatctg cggcttccag
      841 caaaacgcgc cctcgctcca gcaacatcct cccgacattg gtgaatttgg tgcgatggcc
      901 cgagcggtca aacagcacca catccagctc ttcttccagt ttttgcatgg tgtagctaag
      961 tgcggaaggc acgcgtccca gctcatccgc tgccgccgca aaactgcccc ggcgatcgat
     1021 cgcatccata acccgtagtg cttccagcgt taatgccctt tctttggcca tttcgttctc
     1081 attcaggaaa tttgaacata ccgggcagaa tatctggcta acaatgtagc gtccagcccc
     1141 ttaccataaa aggaagtaaa gagaggtcaa gaattatgat tactacccga actgccaggc
     1201 agtgtggaca agcagactac ggatggttgc aggcccggta tactttttcc tttggacact
     1261 acttcgaccc gaaattgtta ggctatgcct ccctgcgtgt gcttaaccag gaagtgctgg
     1321 ccccaggtgc cgcctttcag ccgcgaacct atcccaaagt cgatatttta aatgtgattc
     1381 tggatggaga agcagagtat cgcgacagcg aaggcaatca tgttcaggcc agcgccggag
     1441 aggcgttgct gctctctacc cagccgggtg tcagctatag cgaacacaat ctcagcaaag
     1501 acaaaccgtt aacgcgaatg cagctttggc tggatacctg cccgcagcga gagaatccgc
     1561 tgattcaaaa gctggcgctt aatatggaca agcagcaatt aatcgcctcg ccagagggtg
     1621 cgatgggaag cctgcaatta cgccaacaag tgtggctgca ccatatcgtg ctcgacaaag
     1681 gcgaaagtgc gaatttccag ttgcatgggc cacgcgcgta tttgcaatcg attcacggga
     1741 aatttcatgc gcttacgcat catgaagaga aagcggcgct aacctgcggt gatggggcgt
     1801 ttattcgtga cgaggctaac attacgctgg ttgccgattc cccactgcgc gctttgctga
     1861 tagatttgcc tgtctagttg tttttacagg gagatgatga tgagtaaaaa atcgaccaaa
     1921 aagcgccagc cagcgaagcc cgtggtggcg aaagaacctg ctcgcaccgc caaaaatttt
     1981 ggctatgaag agatgttgag cgagctggaa gctatcgtcg cggatgctga aacgcgttta
     2041 gccgaggatg aagctaccgc gtaaagacag gttgccggat gcggcgtaaa cgccttatcc
     2101 ggcctacaaa acacaacaac atcaacaaat taaacacccc gtaggccgga taagatgcgc
     2161 cagcatcgta tccggcatca ggtacaaaac acaacaacat caacaagttg agcaccccgt
     2221 aggccggata aggtgcgcca gcatcgcatc cggcacaata ctcaaaacct ggcgtttatc
     2281 tggccttgct cgccataatc tcgataatct gccgatccgt ttgctgcatc gaatggcttg
     2341 ctaacgcaca caggttggca atcgactgct caacatcatg cgccacgatc ccttcattgc
     2401 cggtcacggc ggtatcatcc agcgccatta acaccgcttt ccacgcagcc gaagcactgg
     2461 tcgaaacctt catcgcgcag ctgttcgacg caccatcgca aatcatgccg ctgacatcgc
     2521 cgatcatact gctgatcgcc atcgagatag tttcataacg cccatccacc agccatgcca
     2581 tcccggcggc ggcccccatt gctgcggtcg ttgcggcaca aagtgcagac aaacgcggta
     2641 actggttatg gatgtaaatt gcgctcaaat gagaaagcat cagcgcacgc gccagtcgtt
     2701 catcatccgc tccgaagtgt tctgctacaa ccaccacagg cattattgca gtgatcccct
     2761 ggttacccga gccggagtta ctcatagccg gaagcgtagc gccgcccata cgcgcatcgg
     2821 atgccgcgct ggtacgaatc acaatggatg aagagagatc tttcgccagc aaaccgcgct
     2881 cgcactgttt ttccagcgtc gcgccaatat gcagccccca cttaccgctc aaaccttcct
     2941 gcgataacgc acaatttagc ttcgcggaat cgagaataaa gcggatcgcc gcaaatggga
     3001 cttcattgac gaacttcagg atctcagcca gcgtcgttct ggaaagcacc gtcagcggcg
     3061 actcttgctc gccctctgcc acacacgcct ggtgggtaaa caccacacca ttgtgcgtct
     3121 caatatgcac aatgttggta tgcccgccga cgatggtgac acacgcccac ttctcaccgt
     3181 tccagacttt ggcgcgtgag aagaggattt cattgcaagg ttcctggatc ttaacagaga
     3241 ctttccccgc cgccagcagt gctttggcat cggcaattgc ctgcgcagtt gcgtctttca
     3301 gcacttccag cccggcgttg gcatttccac ctaacgcccc cagcgccgcc gcaatcggca
     3361 gccccaccat tcccgtgccg ggaacggtga cgcccagacc gttcttcatc agatttggcg
     3421 aaacccaggc ttctacacgt tcaaccggac cttccagttc tgccgcagca accgccgccg
     3481 ccagcgccag tgaaatcggt tcagtacatc ccagcgccgg ttttacttcc tcctgaacgg
     3541 cgaggatgta acgctgccat aacggattta aagtcgaatc aaacatatta aaaaccttaa
     3601 aatttcaggt aaatcaagaa aatgccagga acagagaaac acaaagcaac aaaccagtga
     3661 cgataatcag gtacagagac atccctttgt atttgtgcaa tgccggtact ttgtaaacca
     3721 gccacgccgg gatcaggcac cctaccatgc cgaaaatcgg gctacagata gaggtgaagc
     3781 tcaacaccgg tgcgttcagt acgatggcgc tccaggccag caaaatggcg aaaatcatga
     3841 tgccgcgctg aacaagattt tcgttaatct tctcggcagg catcttgcga cgcaggatgt
     3901 tcattacgat cccttgcgtt gcttcgcgaa agcctaaata gacgccaaag aatgcagtca
     3961 tgacagcaaa gatattgaga atgacgctga ccactttcac ccacgctgcg ccgtcaccgc
     4021 taataaactg tgcggcaatc gccagcgcgg aaatattctg ctcgtaggct tttaccgctt
     4081 cgtcatgtcc catcgccagc gtgaacgaca cggcgtagaa aaagacggtg acaaacaaaa
     4141 tgccaaacgc gatattcatt gcccgcaatg ctttatgccg cgccacttca attgattttt
     4201 cccgcgagcg ataagagatc accatcgggc ttaacgtctg gataaacaga atcgacgtca
     4261 gggtaaacgg cagcgtaata atggcgtttt tcaccagcag ccccagcggc ggtagcgaac
     4321 cgacgttata cagatgccac attcccacca tcgacacgcc cagcgcagcg actaccagca
     4381 gcttggtcag caccatgtcg gtcgaaattt tgaataacaa tttctcgccg cgtgaagaga
     4441 tcgccaccag aatgcaaatc agtaccagac cataaaaggg actgtctgac agcaaccctt
     4501 ccgtcacgcc gaaggtatgc agataggaag cactatcgtt ggtgatggcg gtggaataga
     4561 cgaacatcca aatcaccagc attacgaaat agagcgcacc taacaggatg ccccagtttt
     4621 tacctaaata accgctaatg acactcgggt aatctttaca ttctggtgat tctgccagcg
     4681 tattaataaa caaccgctga aacagataca ttgccgggta accaatcacc gatgagagca
     4741 aaaataccca caatcccatc aaaccgacct gcaccgggag aaaaacaatc cccgcgccaa
     4801 tcgccatccc gatactcata atcacccagc cggtgtcagt actgtcgaat ttgatcgctt
     4861 ctcgccactc gctctcactc attccagcac gacccgccgg ggtcgaagcg tctgcaatga
     4921 cgcctttatt cgatgcaatt tccataattt ctcgctcaat attttgtagg gcttattatt
     4981 tttttccgag ccgcatcaag gcgatatgcg gtctacgttt ttgcaggcga gttattagaa
     5041 taaagaaatg atacgcgcga ggtcagagaa aatcttcaca atcaagactt taaaataacg
     5101 gagatggata agaattttct actaaattaa tcgcaagaga atattcattc tctatttgcg
     5161 gcagatcaca aaaaaaaggt gcacatttgt gcacccaagg atgaaagctg acagcaatgt
     5221 cagccgcaga ccactttaat ggccagtcct ccgcgtgatg tttcgcggta tttatcgttc
     5281 atatctttgc cggtttcata catcgtctcg atcactttat cgagtgaaac acgcggtgcc
     5341 gaggtgcggc gcatcgccat ccgcgcggcg tttactgctt tcacggcatt aatggcatta
     5401 cgttcaatgc acgggatttg tacctgtccg gcaaccggat cgcaggtcag cccaaggtta
     5461 tgctccatcg cgatttccgc cgcattgcat acctgcgccg gactgccgcc cagtagttca
     5521 gttaaccctg ccgctgccat tgaacaggcc acgccaatct ccccctgaca gccgacctcc
     5581 gcgccagaga tggaggcgtt cattttatac agcgcgccaa tagcccccgc tgccagaaaa
     5641 tagcgggcaa ttgaccgctc gtttaccgga cgacggaact tatcgtaata agccagcact
     5701 gccggaataa tgccgcacgc accgttagtc ggtgccgtta ccacgcgccc gccagctgcg
     5761 ttttcttcac taaccgccag cgcgtacatg ttgatccagt cgatgacatt catcggatcg
     5821 ttagagatgt tatcgctaga aaccagctga cgacgcagcg ctacggcacg acacggcaca
     5881 ttgagcggac caggcagcac gccttcagtg ttcatcccgc gttcaatacc gtcatgcatc
     5941 acttgccaga tacgggcaaa ccctgcgtca atttccgctt tgctgcgcag cgccagctcg
     6001 ttgtgcatca tcaggccaga tatagacagg ccgttgtaat cacacatttt cagcagttca
     6061 cctgctgagt ggaaatcgta aggtacggac gtttcgacat cgtgcgacag gccgaagtgt
     6121 tcttcttcga caataaaccc gccgccgaca gagtaatagg ttttacttaa tagcgcttcc
     6181 tgtcccttcc aggcagtgat ccgcattccg ttctcatggc gaggcaacat ttcgggatgg
     6241 aagataatgt tctttgctac aggaaaatca acaatatgcg caccagatgc cactggcagc
     6301 cgcccgctgc gcgttactaa ctctataaat gcagggatct catcaatgac aacatcctgc
     6361 ggactgtttc ctgccagtcc catgataatg gcgacatccg tggcatggcc tttgcccgtc
     6421 agtgacaacg acccgtacag atcgaccaca atatggctcg tcgcggttaa taagccgcta
     6481 ctttccagcc gatcaataaa actttttccg gcattcattg gccccacggt atgcgaactg
     6541 gagggaccaa tcccaatttt gaaaatatcg aatgcactaa tcatatccac accctcggat
     6601 tgccgttcag tgaagtggag cggaacgacc ttgcgaccgt cccgctcacg aggctttacg
     6661 cactacgtac tgcgatagct tcaatttcca gcttcacatc tttcggcaaa cgcgcaacct
     6721 gcacacagct ccgggtcggg taggtcgcct gatgctcatc gaagaactgc ttatagactt
     6781 cattgatggt ggcaaaatca ttcagatcgg tgatgaacac ggtcatcttg atgatatcgc
     6841 ccacgctcag cccggcagca accacgatcg ctttgacgtt ttcgaggctt aaacgtgcct
     6901 gatcttgcac atcagccggg atctcaccgg tctgcgggca aaccggaatt tgcccggagg
     6961 tgaagaccat gctgcctaaa tcaacgccct gaacataagg gccgattgcg cctggggcac
     7021 gttgcgtttc gataatcttt ttcatacatc ctccggcgtc agagcgcctg ggtaaaagta
     7081 cgtgaaataa catcctgttg ctgttcacgg gtcagtgcgt tgaagcgcac ggcgtagcca
     7141 gagacacgga ttgtcaggtt aggatatttt tccgggtgct cgatggcatc cagcagcatt
     7201 tcccgattca ttacgttgac gttgaggtgt tgaccgcctt cgacatccgc ttcgtggtgg
     7261 aaatacccat ccagcaggcc aacaaggttg gttttacgta ctggatcttc tttgcccagc
     7321 gccgcaggaa cgattgagaa ggtgtacgag atcccatctt tggcgtaggt gaacggcagt
     7381 ttcgccaccg acgtcaatga ggccacggca cctttgcggt cacgaccatg catcgggtta
     7441 gcgcccggcg cgaacggtgt tccggcgcga cgaccgtccg gcgtattacc ggttttctgg
     7501 ccgtacacca cgttagaagt gatagtcaga atcgactggg tagggacggc gttgcgatag
     7561 gttggcagcg ctttaatttt cttcataaag cgttcaacca ggtcgcaggc aatgctgtct
     7621 acgcgctcgt cgttgttgcc gtactgcgga tattcaccgt cgatttcaaa gtccaccgcc
     7681 aggccgtttt cgtcacggat tggtttcacg cgggcatatt tgatggcaga cagggagtcc
     7741 gtcgccaccg acaggcccgc gatgccgcat gccatagtgc gatagacatc acgatcgtgc
     7801 agcgccatca gcgaagcttc gtagctgtac ttgtcgtgca tgtagtgaat gatattcagc
     7861 gcgctgatgt actgcaccgc cagccagtcc atgaagtgat cgaggctgtc catcactttg
     7921 tcgtaatcca gcacgtcgtc catcagcggt gctgttttcg gcccgacctg aatcttcagc
     7981 ttctcgtcca ccccgccgtt aattgcgtag agcagcgttt tcgccaggtt agcgcgtgca
     8041 ccaaagaact gcatttgctt accaatcacc attgggctga cgcagcaggc aatcgcgtaa
     8101 tcgtcgctgt tgaagtcagt acgcatcaga tcgtcatttt catactgcaa ggaagaggtg
     8161 acgatcgaca cctgcgcggc atattttttg aaggcaatcg gtaattcttc cgaccaaaga
     8221 atggtcaggt taggttccgg tgccggcccc atagtgtgca gggtgtgcaa atagcggaag
     8281 gagtttttgg tcaccagcgt acgaccgtcc agccccatcc cgccgatcac ttccgtcgcc
     8341 cagattgggt cgccggagaa cagcgaatca aattccggtg tacgcaggaa gcgtaccata
     8401 cggatcttca tgatgaagtg atcgatcagt tcctgtgcct gctgctcatt gaggacgcca
     8461 gctttaaagt cgcgctcaat gtagatatcc aggaacgatg ccgtgcgccc cagcgacatc
     8521 gcgccgccat tttgcgattt cactgccgcc agataagcga agtagagcca ctgcaccgct
     8581 tcctgtgcat tctgcgccgg gcgagagata tcaaagccat atttcgccgc catttcctga
     8641 atctgcaaca gcgcatgacg atgctctgcc agctcctcac gcagacggat ggtggcttcc
     8701 agatcctcgc ctttttccag acgagactgg agatcggcaa attgcagttc gcgttcacgc
     8761 accagataac tgatgccata cagcgctacg cggcgatagt caccgataat gcgcccacga
     8821 ccatagccat ctggtaaacc ggtcagcacg ccagatttac ggcagcgcag catatccggt
     8881 gagtaaacat caaatacgcc ctggttatgg gttttacgca gatcggtaaa cagatattca
     8941 aattcactgt ccatttcacg gccataggcg tggaatgaac ttttaatcat attaatgcca
     9001 ccgaacgggt gtagcgcacg tttcaacggc gcatccgttt gcaggccaac aattttttcc
     9061 agcggctggt taatatatcc cgcatcatga gcggtaattg tggtggcaat attggtatcg
     9121 aaatcaaccg gcgcgtgggt tgcgttttcg atacggatgc cttccattac cttttcccac
     9181 aattccgtgg tggcaggcgt cgcttcggcg agaaaagatt catcgccttc atacggtgta
     9241 tagttatgtt gaataaaatc gcggacatta atttcgcttt tccagtccgt acctttaaag
     9301 ccaagccatg cgtcagcgta cagcttatcg ctggtatcaa tatctacctt catgaaaaat
     9361 aatctctcta caatacttca actaaattat gcaaattctg cgggcgcgtt aactttacct
     9421 aaatgaatgg catccaaagc aatcattttt tcttcgttag tcggaataac gacgcaaatg
     9481 acatgcgcat tttcactgga aacaattcgc tcgccaaaag agttagatcg attattcatt
     9541 tctgtatcaa tctctacgcc taatacagcc aaatgttcca tgaccagacg acgaattaaa
     9601 cttgaattct ctcctattcc gccggtgaat ataattccat ccagccgacg taatgaagct
     9661 gcatgtccgg caatatgacg ggcaattcgg tgaacaaagg ttttaattgc cagttgcgcg
     9721 cgttcgtgac cttcatgcca ggctttttcc agaacacgta aatccgaaga aagaccggaa
     9781 atacctaata atcccgactc tttattcact acgcgttcca ggtcacccag gctctggttg
     9841 gtttggctgg cgacccagga catcgcacca aagtcgacat cgccactgcg ggtacccatc
     9901 atcaagcctt ccagcggcgt cattcccatt gaggtgtcaa cactctgacc gttgcgaact
     9961 gcgcagattg acgcgccatt gccaagatgc gccacaacca ggccggagtc atcttccgcc
    10021 agattcagca gcgaatgtgc gcgctgggaa acatagcggt gcgacgtgcc gtggaaacca
    10081 taacggcgta cacctaactc ttcataatat ttccacggca ggccgtataa ataagcttcc
    10141 ggagccatcg tctggtggaa actggtatca aataccgcca cctgagttac gcccggaaat
    10201 aattgctgcg ccgattcaat accacttaaa tttgcgtaat tatgcagggg tgccagtgga
    10261 gaaacgcgac ggatattatc aatgacttca tcggtaataa tggcggactc ggtaaaaata
    10321 ctgccgccgt gagcgatgcg gtggccaatt aaggccacgc tgtcatttaa attccgtttt
    10381 tccagttcaa atgcaattgc cttcaatgca ccttcgtagc tgtggtgagc cagcggtgct
    10441 ggctctcccc catttacgga taagaatgca ttttccgagt taataccgtc ggcaatacct
    10501 gacattaata cttcacaatc gctggcatcg agtacggaaa acttaatcga agacgaacca
    10561 cagttaataa ccaaaacaac cggaaattca ttcatctctt ttctcatcct gagttacgga
    10621 ttaaaacagt ttgtatacga tgttcaggat ggtcagcaga ccaatcacgg taacaaacac
    10681 gttatccaga cgaccacggt atttcgccag agacggcgct ttacggatag catacatcgg
    10741 caacaggcac agcagggatg cgataatcgg tgcgcccatg gcttcaatca ggtcgaggat
    10801 gttcgggttg gcgtaggcaa caacccaggt ggagcccatg atgaagatca tgctgagagt
    10861 attcagttta cccagcgaca ctttggtttt gtcaccttta taaccgaact tcagaatcag
    10921 accattcaag ccttccagcg tccccagata gtgaccgaag aaagatttga agatagccac
    10981 gagtgcgatg atggaagccg catattccag tgtaatcgcg aacgttgttt tcgtaccggt
    11041 catggacgca aagtggttag ccagataaga aagcactgga atattctgcg ctttggcttc
    11101 cgccatgttg gccggagaca gagtaaacag gcagctaaag gcaaagaaca tcaccactgc
    11161 aaccatcagc atgctggcac gagaaatgat ttgggaacat ttacgttcgg tgaagtcgcg
    11221 accgaagtct ttctcatact cttcacgttt agaaaccacg aaggaagaga cgattggcga
    11281 gaagttaaag gagaaaacca tgatggaaat ccccagccag acagtgatca ggataccgtc
    11341 atgaccggtt aacgacagcg aaccgaggtc aacctggtcg ataactgcag agttccagta
    11401 agggatcagc gacagagaaa tcagcaccag gctggcgata aacggccata ccaggtagct
    11461 catcacttta accatcagat ccttaccaaa ccagatgacg aaagccatca gcagcaacag
    11521 gaacagcgcc acaaagccgc gattcagcgg tgcaaatccg agctggtttt cccagaacgt
    11581 cataaaggta ttggtaatag taacgccata aatccacagc agtgggcaaa tcgcgaagaa
    11641 gtacaggaac gtgataacca cgccgccagt tttaccaaaa tgctcttcca ccgtttccgt
    11701 aatgttgccg gaagggttag agccagaaag acacagacgc gccagcgccc ggtggcaata
    11761 aaatgcgatg gggtatgcca ataccaacat cagaagaatc gggatcagtc cgccaaaacc
    11821 tgcgcggata gggaagaaca gcaccccggc gccgattgcc gtaccaaaca agcctaacgt
    11881 ccatgtggta tctgatttac gccaggacga ttgttttgtc tggctggata caatgctatc
    11941 tgaagtactc atatcctatc ctcaacgaat taattaagcg tcaacgaaac cggtgatttg
    12001 agagacgcga gaaagatcga tattgccgcc ggaaataata ctgacggttt ttctgttttg
    12061 aatatattgg tctaatttac cgcttaataa tgcagcacat gccagagcgc ctgcgccttc
    12121 ggtgacgact ttattgcgct gaattaaggc aatcatactg tttctgattt cgtcttcgct
    12181 gaccagcacg atgtcatcga ctaattcacg aacgatttcg taagttaaat tacccgggcg
    12241 ggagacatca caaccatccg ccagggtgcc ggtagttcgg tgcgtggtta tttctccgga
    12301 gtggaaagaa gccgccatgc cgtgaacgtt ttctgactgt acgccaataa cacgaatggt
    12361 cgggttaata gatttaattg ccaccgcaat accagcaatt aaaccaccac caccaattgg
    12421 cacaatcacg ttatcgacat catagagatc ttccataatt tccagaccaa tcgttccctg
    12481 gccagcaatc actttcggat catcgtaagg tgggataaaa atacgacctt ccatttcgac
    12541 aatttcgctc actttagcga tagtgtcgtt gaagttatca ccatgcagaa cgacttctgc
    12601 ggagtagtcg cacgttgccg ctactttgga ttttggcgca ccttttggca tcaccacttt
    12661 accgtcgata cccagcatcg cgcaggagag cgaaacccct tgcgcatggt tgcccgcaga
    12721 acaggccacc acgcctttgc gtttttccgc atcggtcagt gaacttaatt tattaaatgc
    12781 gccacgaatt ttaaatgaac ccgtacgctg catattttca aacttcagga atatttcacc
    12841 tttgcaacgt tcactaaaat agttggagcg aggcatgcct gttttataaa ttcgcccagc
    12901 cagtcgttgt ttcgcttcaa taatatcatc aatagcaacc ggcagatcgt atgtaatatg
    12961 cattataaaa cctcttcgcc tgaattaaat atgtaggtaa ccgtaaccga cacctgcaaa
    13021 acagacaggt ggattattta tgtttaaata aaaacaaaca actaaccaac ttctattaat
    13081 tgccttcgtc tacacccatt ataagatgaa tactctttgg ctaattccac caaaaccgat
    13141 gctgcttttt taatacgata atttttcgac cataccgcgg catattgtgc cacaggtaat
    13201 gtttcttcaa ccggaatagt aataaattga ttagaaccaa aaggtgacgt catatcacaa
    13261 ggaattacag ttaagaaatc agcattgaga acaagattat aaattgtcac gactgagtcg
    13321 gttttaacga tgttttcaat actgatgcca tttctttgta acgtagtaag cagttcgctg
    13381 tagtacccca tattagtttg tggcaacacc cactgttcgt tcttcaacga ctccagcgtg
    13441 gtggtgccgg tgcatgttcg ggacttactg gctaccagca caaactcgga ctcgaacagc
    13501 ggctcaacat gtaaatcctg aagcttcatt tctgcactta acgtaccaat cgcaaaatcc
    13561 agtcgaccgt cgcggattgc cggtaagaac gaagacagtt gcgcttcata catagaaacc
    13621 tgcgctttcg ggaacacctc tttgaacttg ttgatcatcc ctgacataaa agtaaaacca
    13681 atcaatgaag gaaaaccaaa tgagacttcc accaccgctt cagaagacat accgcttatc
    13741 tcattaacca tatttttcat ttcacgggta atggattcgg aacgggagag taacaattga
    13801 ccggcaggtg ttaatgttac accggtattt ttccgcacca ctaattccac accaaaataa
    13861 tcttcaatat cgttaatgat tttactgacg gccggttgag ttaaccctaa ttcttttgca
    13921 gccgagccga tagagccact tctaatgact tcctgaaaga ctaccaggtg ctgcgttttc
    13981 ggaagaagaa tagtgctcat aacgacctac gttaattacc tcattgacgg catgaagtgt
    14041 atcaaaatga aataaacagg atatgtgcga ccactcacaa attaactttc attactttcc
    14101 aaggtatcgt tattacaatt aaatatcttt aaatatcaac aagttaaagc ataaaaatca
    14161 gcataaaccc tgatttttat caaaaaaaaa tgacggggat aaccatattt tatggcgata
    14221 acatcattcg ttatgtggtt aatacaaaaa aggggctgag accatatttc aagcaattac
    14281 cggataatta ccaggcaaaa tttgagttaa atttaatgag caagttctca aattttatta
    14341 taaataaacc attttcagcg ataaatactg cggcacgtca cattttttca cgctatttgt
    14401 tggagaacaa atatttattt tatcaatatt ttaaaatttc gaatacaggt attgatcatc
    14461 tcgaacaatt gattaacgtc aactttttct cttctgacag gacgtcattt tgtgaatgca
    14521 atcgttttcc ataaattctt ctcccctcat aggcgacgaa tagcattttg tgttgaggat
    14581 cacaaaacga ataattgctg gtcgccgcga taaggtcaga cgaagtcaac aagggggatt
    14641 ttcactgtgc tttacatcag cgtaggacac agaatgttgc ttcgtttact gtaacgccgg
    14701 gtaaatgagc gttttttgat agtgcgtaag tgtcgtgcga ggaaaataag catctattat
    14761 tgtttgtcat gacgacaagg caggcatcaa tgaaaggctt cccaattgca catatttttc
    14821 acccttcaat cccgccaatg cacgcagtgg ttaacaatca caatagaaat attgattatt
    14881 ggacggtaaa aagaaagttt gcagaaattg tctccaccaa tgacgttaat aaaatttaca
    14941 gtataagtaa tgaactccgg agagtattat ctgcaattac tgcattgaat ttctatcaag
    15001 gcgatgttcc ttctgtcatg atccgaatcc aaccggaaaa tatgagtcca ttcatcatag
    15061 atatttctac aggagaacat gatgattata tcatacaaac attagatgta ggcacttttg
    15121 caccttttgg tgaacaatgt acttgctcag ccgtcaataa aaaagagctg gaatgtatta
    15181 aggagacgat ttctaagtat tgcgcaaagt tcacccaaaa agaagccatc ttaacacccc
    15241 ctaggcactt taataaaacc tgcattactt cagattgttg gcaaattctc tttttttcac
    15301 ccgatcattt taataatgat ttttattgat tatttataaa ggataaaata atgttccctg
    15361 tttcatcaat tggtaatgat attagtagtg atttagtccg tcgaaaaatg aatgaccttc
    15421 ctgaaagccc cataggaaat aacctcgaag cactagcacc aggtatagaa aaactaaaac
    15481 agacctctat tcaaatggtc actttactta acgcgttaca accaagtgga aaatgcatta
    15541 tcactggtga ttttcaaaaa gaattagcgt acttacaaaa tgtaattctt tataatgact
    15601 cgtctcttcg tctggatttt ttaggttata acacccaaat tattcaacga tcggacaata
    15661 cctgcgaact taccattaat gaaccgttaa aaaactagga aatatctaca ggcaatatca
    15721 atgttaattg cccattaaaa gatatttaca atgaaatcag gaggttgaac gtaatcttta
    15781 gttgtgggac tggagatata gttgatctat cctctctgga cttacgtaat gtcgatttag
    15841 attattatga tttcacagat aaacatatgg ctaatactat tctaaatcat tttaaattgg
    15901 attcaacaaa ttttactaat gccaacatgt ttcaggttaa ttttgttagt tcaacacaaa
    15961 acgccacaat ctcctgggat tatttactaa aaataacgcc tgttttaata agcattagcg
    16021 atatgtattc tgaagaaaaa atcaagtttg tcgaaagttg tttaaatgag cttggagaca
    16081 ttaccgaaga acaattaaaa attatgagat ttgcaattat aaaatctata ccaagggcaa
    16141 ctcttacaga taaattagaa aatgaattaa caaaagaaat atataaaagc tcatcgaaaa
    16201 tcaacaattg cttgaacaga attaaattac cagagatgaa agaattctca tcaaaaaaaa
    16261 tatatgatta catcgataca atcattgaag attatgaaaa tatcaaagaa aatgcttatc
    16321 tggtcgtccc ccaaattaat tatactatgg atttaaacat agaagactct agctcagaag
    16381 agttaatacc ctcgagaaag acgaaaattc tccggacaat ggctttgagg tcggggaata
    16441 taacacatat gaagcatata actcagagaa tcaatatttt accagagagg actatacgta
    16501 taattacgac cttttaaatg caatatagac ttaataactt cttacaaagt ctattattaa
    16561 taccgtagat attcatctta ccctgtgcac caaaagcaaa aacttatcga cgcgggtttg
    16621 aaactgatgg caaccgcaaa aatgcctgat gcgctacgct tatcaggcct acgccatctc
    16681 tgcaatatat tgaatttgcg tgcttttgta ggcaggataa ggcgttcacg cagcatccgg
    16741 catgaataaa gcgcactcaa caatctgaaa cccgccggaa cggtttatta cgtacatcag
    16801 gtgaaactga ccgataagcc gctttctttt gggtatagtg tcgtggacag tcattcatct
    16861 ttctgcccct ccaaaagcaa aaacccgccg aagcgggttt ttacgtatat caggtgaaac
    16921 tgaccgataa gccgctttct tttgggtata gcgtcgtgga cagtcattca tctttctgcc
    16981 cctccaaaag caaaaacccg ccgaag
//
LOCUS       DAJTQY010000083        16034 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.083, whole genome shotgun sequence.
ACCESSION   DAJTQY010000083 DAJTQY010000000
VERSION     DAJTQY010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16034
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.083"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(<1..1708)
                     /gene="speA"
                     /locus_tag="OO349_002919"
     CDS             complement(<1..1708)
                     /gene="speA"
                     /locus_tag="OO349_002919"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HCR5867918.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPVE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQ"
     gene            complement(1717..1848)
                     /gene="yqgB"
                     /locus_tag="OO349_002920"
     CDS             complement(1717..1848)
                     /gene="yqgB"
                     /locus_tag="OO349_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HCR5867919.1"
                     /translation="MKKKPVAQSERQHTLLENPCAYGLLSQFQAATVVNCFTLNKII"
     gene            1984..2199
                     /locus_tag="OO349_002921"
     CDS             1984..2199
                     /locus_tag="OO349_002921"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5867920.1"
                     /translation="MGITSAGMQSRDAECGERVFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            2503..3657
                     /gene="metK"
                     /locus_tag="OO349_002922"
     CDS             2503..3657
                     /gene="metK"
                     /locus_tag="OO349_002922"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HCR5867921.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            4094..5488
                     /gene="galP"
                     /locus_tag="OO349_002923"
     CDS             4094..5488
                     /gene="galP"
                     /locus_tag="OO349_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HCR5867922.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGIGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            5565..6062
                     /locus_tag="OO349_002924"
     CDS             5565..6062
                     /locus_tag="OO349_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HCR5867923.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAIYRCVHCGE
                     QLVAK"
     gene            6157..6864
                     /gene="endA"
                     /locus_tag="OO349_002925"
     CDS             6157..6864
                     /gene="endA"
                     /locus_tag="OO349_002925"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HCR5867924.1"
                     /translation="MYRYLSIAAVVLSAAFSGPTLAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            6944..7675
                     /gene="rsmE"
                     /locus_tag="OO349_002926"
     CDS             6944..7675
                     /gene="rsmE"
                     /locus_tag="OO349_002926"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003853382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HCR5867925.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            7688..8638
                     /gene="gshB"
                     /locus_tag="OO349_002927"
     CDS             7688..8638
                     /gene="gshB"
                     /locus_tag="OO349_002927"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HCR5867926.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTCYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            8747..9310
                     /locus_tag="OO349_002928"
     CDS             8747..9310
                     /locus_tag="OO349_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HCR5867927.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIAGRWREAAKLIGVDILTMPGVAGHA"
     gene            9310..9726
                     /gene="ruvX"
                     /locus_tag="OO349_002929"
     CDS             9310..9726
                     /gene="ruvX"
                     /locus_tag="OO349_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HCR5867928.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(9902..10882)
                     /locus_tag="OO349_002930"
     CDS             complement(9902..10882)
                     /locus_tag="OO349_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HCR5867929.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQRVGEGRL"
     gene            10900..11604
                     /gene="yggS"
                     /locus_tag="OO349_002931"
     CDS             10900..11604
                     /gene="yggS"
                     /locus_tag="OO349_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HCR5867930.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNEQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            11622..12188
                     /gene="yggT"
                     /locus_tag="OO349_002932"
     CDS             11622..12188
                     /gene="yggT"
                     /locus_tag="OO349_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HCR5867931.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVA
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFEVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYAINMGVAEVLQATGNMLLPGLWMAL"
     gene            12185..12475
                     /locus_tag="OO349_002933"
     CDS             12185..12475
                     /locus_tag="OO349_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HCR5867932.1"
                     /translation="MSAVTVNDDGLVLQLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            12483..13076
                     /locus_tag="OO349_002934"
     CDS             12483..13076
                     /locus_tag="OO349_002934"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HCR5867933.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            13069..14205
                     /gene="hemW"
                     /locus_tag="OO349_002935"
     CDS             13069..14205
                     /gene="hemW"
                     /locus_tag="OO349_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HCR5867934.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            14519..15482
                     /locus_tag="OO349_002936"
                     /pseudo
     CDS             14519..15482
                     /locus_tag="OO349_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            15527..16030
                     /locus_tag="OO349_002937"
     CDS             15527..16030
                     /locus_tag="OO349_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HCR5867935.1"
                     /translation="MIFNRLKLAVDRVIAAFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAG
                     SVIVTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGVVMMFYALCFINQSIQQLK
                     QPAQEAS"
BASE COUNT         3957 a         4136 c         4054 g         3887 t
ORIGIN      
        1 cctgatatgc accgaccata aagaaaccga gcatcggcgg attctctgga tcgtactccg
       61 gcattggcat tgtcgtggca ataccatcac catcaatata gtggtcgata gcaccgtcag
      121 agtcacaggt aatatccagc agcacagcgc gacgttccgg cacttgatcc agcccttcca
      181 gcggtagaac cgggaacaac tggtcgatcc cccatgcgtc cggcattgac tggaacagcg
      241 agaaattgac gtacattttg tccgccatac gttcctgcag ttcgtcgatt atcggacgat
      301 gcgcacggtt ttgcggatcc agttgcttct gcacttcatg gcacatgctc agatagagtt
      361 gttctgccca tgcacgttct tgcaggctaa aggtgccgga agagtagccg atatgaatgt
      421 cgtgcagatc catctggctg tcgtgtaacc attcacgcaa agaacggcga gttcccggtt
      481 cgtgcatttc ctgccaggtt tcccacatgc tttgcagcgc acgcggcgca tcttctacgg
      541 gcgcggtcgg caccgtatat tcgttacgtt ccacgccgat gatattcgac accagcacgg
      601 tatggtgcgc agtcaccgca cggcccgatt cggtgattac cgtcggatgc ggcagaccat
      661 tttcttcaca cgcatcgcca atcgcccaga tgatgttgtt ggcgtattca ttgaggccat
      721 agtttacgga gcagtcagac tgcgaacggg tgccttcata atccacgccc agaccgccgc
      781 cgacgtcgaa gcactgaata ttgacgccca gcttgtgcag ttccacatag aaacgcgcgg
      841 attcacgaac gcctgtcgcg atatcgcgaa tattcgccat ctgcgaaccg aggtggaagt
      901 gcagtagttg caggctgtcg agacgcccgg cttcacgcag ggtttcaacc agttgcagta
      961 cctgagtcgc agccaggccg aacttcgatt tttccccgcc ggaggactgc catttacccg
     1021 agccctgcga agccagacgt gcacgcacgc ccagacgagg aacgacattc agacgttctg
     1081 cttcatccag cacaatggcg atttctgaca tcttctcaat gaccagatag accttgtgcc
     1141 ccatcttctc gccaattaat gccaagcgga tatattcgcg gtctttataa ccgttgcaga
     1201 cgatgacgct acgagtcatg ccagcatgtg ccagcactgc catcaactcg gctttggaac
     1261 cggcttccag acccagcggt tcacccgaat gaatcaggga ctcgatcacg cggcggtgct
     1321 ggttaacttt gatcggataa acaaggaagt aatcgccgtt atagccgtag gattccctcg
     1381 cacgtttgaa cgcggcgtta atggaacgca aacggtgctg caggatctgt gggaaacaga
     1441 acagtgcagg cagacgctga ccctgtgctt cacgagtttt cactaactgc gcgagatcga
     1501 cgcgagcttc cgggacgtcc gggtccgggc acacgctgat gtggcccagc tcgttaacgt
     1561 catagtagtt attgccccac caggcaatat tgtaagtacg cagcatcttg ctggcttcct
     1621 gggagctcat tgcaacctcc tgcatggagc gtagtacacc gtgttcgccc gctgacgaag
     1681 gcaaacccat agacatgtcg tcagacatag cgaacctcaa attattttat taagtgtaaa
     1741 acagttaacg actgtcgcag cctggaactg cgataacaac ccataagcac atggattttc
     1801 cagcagtgta tgctgacgct ccgactgcgc aaccggtttc tttttcataa cattattaag
     1861 cacataaccg aacgtaagtg tgacagttcg gcgaaaccac gagaaaactc ttgtttttac
     1921 aagagcgccc ttgttcagtc ctcagtaact gtaaccagct cttgaatcct gagaagcgcc
     1981 gagatgggta taacatcggc aggtatgcaa agcagagatg cagagtgcgg ggaacgagtc
     2041 ttcaccagaa cggtgagaca ggttaagcag cagacaacgg ttcattattt cgtatcacct
     2101 ccacggccgc ctgttaagac gaacccacaa gccaaaactc tgatttcaac ccggctggaa
     2161 gtggcaacac gaaagaaacg tcgtgtgctt tttatttaag ccgcgcgccg cgttttatac
     2221 cccacaatgg cagaaaattg caaaagataa atacgcagaa tgccggcatt gtcaggaaaa
     2281 atttccagcc acgtttttaa cagaatgaga cacgattcaa aaaaaagtgg aaatcgggtg
     2341 aagaattgac ctaaaatagc catccagatg ttaatccatc cataccgatt aacactcaga
     2401 ctgccagtgt ttttaacctg cagagtcgtg gtaggatccg ctaccacaga aaatccacac
     2461 aacagtttga gctaaccaaa ttctctttag gtgatattaa atatggcaaa acaccttttt
     2521 acgtccgagt ccgtctctga agggcatcct gacaaaattg ctgaccaaat ctctgatgcc
     2581 gttttagacg cgatcctcga acaggatccg aaagcacgcg ttgcttgcga aacctacgta
     2641 aaaaccggca tggttttagt tggcggcgaa atcaccacca gcgcctgggt agacatcgaa
     2701 gagatcaccc gtaacaccgt tcgcgaaatt ggctatgtgc attccgacat gggctttgac
     2761 gctaactcct gtgcggttct gagcgctatc ggcaaacagt ctcctgacat caaccagggc
     2821 gttgaccgtg ccgatccgct ggaacagggc gcgggtgacc agggtctgat gtttggctac
     2881 gcaactaatg aaaccgacgt gctgatgcca gcacctatca cctatgcaca ccgtctggta
     2941 cagcgtcagg ctgaagtgcg taaaaacggc actctgccgt ggctgcgccc ggacgcgaaa
     3001 agccaggtga ctttccagta tgacgacggc aaaatcgttg gtattgatgc tgtcgtgctt
     3061 tccactcagc actctgaaga gatcgaccag aaatcgctgc aagaagcggt aatggaagag
     3121 atcatcaagc caattctgcc cgctgaatgg ctgacttctg ccaccaaatt cttcatcaac
     3181 ccgaccggtc gtttcgttat cggtggccca atgggtgact gcggtctgac tggccgtaaa
     3241 attatcgttg atacctacgg cggcatggcg cgtcacggtg gcggtgcatt ctctggtaaa
     3301 gatccatcaa aagtggaccg ttccgcagcc tacgcagcac gttatgtcgc gaaaaacatc
     3361 gttgctgctg gcctggccga tcgttgtgaa attcaggttt cctacgcaat cggcgtggct
     3421 gaaccgacct ccatcatggt agaaactttc ggtactgaga aagtgccttc tgaacaactg
     3481 actctgctgg tacgtgagtt cttcgacctg cgcccatacg gactgattca gatgctggat
     3541 ctgctgcacc cgatctacaa agaaaccgca gcatacggtc actttggtcg tgaacatttc
     3601 ccgtgggaaa aaaccgacaa agcgcagctg ctgcgcgatg ctgccggtct gaagtaatct
     3661 ttcttcacct gcgttcaaag gccagcctcg cgctggcctt tttcttttac ctcaccgcgg
     3721 tggataggcg ttcacgccgc atccggcaaa aaaactgccc gcacaataac atcattcttc
     3781 ctgatcacgt ttcacggcaa attaccatca caactgaaac cgattacacc aaccacacca
     3841 gcaaaatatt tgtaatattt tcatattatt attcagcttt cacagttgtt acatttcttt
     3901 tcagtaaagt cttaattgca gatgacagcg tttaatctat gatgatataa ctcaattatt
     3961 ttcatgccct taaatcataa ctaagataaa tgttagtgta agcgattaca ctgatgtgat
     4021 ttgcttcaca tctttttacg tcgtactcac ctatcttaat tcacaataaa aaataactat
     4081 attggagggc atcatgcctg acgctaaaaa acaggggcgg tcaaacaagg caatgacgtt
     4141 tttcgtctgc ttccttgccg ctctggcggg attactcttt ggcctggata tcggtgtaat
     4201 tgctggcgca ctgccgttta ttgcagatga attccagata acttcgcaca cgcaagaatg
     4261 ggtcgtaagc tccatgatgt tcggtgcggc agtcggtgcg gtgggcatcg gctggctctc
     4321 ctttaaactc gggcgcaaaa agagcctgat gatcggcgca atcctgtttg ttgccggttc
     4381 actgttctct gcggctgcgc caaacgttga agtactgatt ctttcgcgcg ttctgctggg
     4441 gctggcggtg ggtgtggcct cttataccgc accactgtac ctgtcagaaa ttgcgccgga
     4501 aaaaattcgc ggcagtatga tctcgatgta tcagttgatg atcactatcg ggatcctcgg
     4561 tgcttatctt tctgataccg ccttcagcta caccggtgca tggcgctgga tgctgggtgt
     4621 gattatcatc ccggcaattt tgctgctgat tggtgtcttc ttcctgccag acagcccacg
     4681 ttggtttgcc gccaaacgcc gttttgttga tgccgaacgc gtgctgctac gcctgcgtga
     4741 caccagcgcg gaagcgaaac gcgaactgga tgaaatccgt gaaagtttgc aggttaaaca
     4801 gagtggctgg gcgctgttta aagagaatag caacttccgc cgcgcggtgt tccttggcgt
     4861 actgttacag gtaatgcagc aattcaccgg gatgaacgtc atcatgtatt acgcgccgaa
     4921 aatcttcgaa ctggcgggtt ataccaacac caccgagcaa atgtggggga cagtgattgt
     4981 cggcctgacc aacgtacttg ccacctttat cgcaatcggc cttgttgacc gctggggacg
     5041 taaaccaacg ctaacgctgg gcttcctggt gatggctgct ggcatgggcg tactcggtac
     5101 aatgatgcat atcggtatcc actctccgtc ggcgcagtat ttcgccatcg ccatgctgct
     5161 gatgtttatt gtcggttttg ccatgagtgc cggtccgctg atttgggtac tgtgctccga
     5221 aattcagccg ctgaaaggcc gcgattttgg catcacctgc tccaccgcca ccaactggat
     5281 tgccaacatg atcgttggcg caacgttcct gaccatgctc aacacgctgg gtaacgccaa
     5341 caccttctgg gtgtatgcgg ctctgaacgt actgtttatc ctgctgacat tgtggctggt
     5401 accggaaacc aaacacgttt cgctggaaca tattgaacgt aatctgatga aaggtcgtaa
     5461 actgcgcgaa atcggcgctc acgattaatc tcctcaggct tcctcccgtc gcggaggaag
     5521 ccgcctcttg cagtcatctt ttcttcgctc tatcctctgc cgctatgaaa acatcccgtc
     5581 tccctatcgc catccaacag gccgttatgc gccgcctgcg ggaaaaactc gcccaggcca
     5641 acctgaagct agggcgtaac tacccggagc caaaactctc ttacacccag cgcggaacct
     5701 ccgccggaac agcctggctg gaaagctatg aaatccgcct caatcccgtt ttgctgttgg
     5761 aaaacagtga agcttttatt gaagaagtgg taccgcacga actggcacat ttgctggtat
     5821 ggaaacattt cggccgcgta gcgccacatg gcaaagagtg gaagtggatg atggaaagcg
     5881 tgctgggcgt tcccgcccgt cgtacgcatc agttcgaact gcaatccgtg cgtcgcaaca
     5941 ccttccccta ccgctgtaag tgccaggaac atcagcttac cgtacgccgc cataatcgcg
     6001 tagttcgtgg cgaggccatc tatcgctgtg ttcactgcgg tgaacagctg gttgcgaaat
     6061 aaccacctga actatcagga actttcctga tctggctgat tgcataccaa aacaactttc
     6121 gctacgttgc tggctcgttt taacacggag taagtgatgt accgttattt gtctattgct
     6181 gcggtggtac tgagcgcagc attttccggc ccgacgttgg ccgaaggtat caatagtttt
     6241 tctcaggcga aagccgcggc ggtaaaagtc cacgctgatg cgcccggtac gttttattgc
     6301 ggatgtaaaa ttaactggca gggcaaaaaa ggcgttgttg atctgcaatc gtgcggctat
     6361 caggtgcgca aaaatgaaaa ccgcgccagc cgcgtagagt gggaacacgt cgttcccgcc
     6421 tggcaattcg gtcaccagcg ccagtgctgg caggacggtg gacgtaaaaa ctgcgctaaa
     6481 gatccggtct atcgcaagat ggaaagcgat atgcataacc tgcagccgtc agtcggcgag
     6541 gtgaatggcg atcgcggcaa ttttatgtac agccagtgga atggcggcga aggccagtac
     6601 ggtcaatgcg ccatgaaggt cgatttcaaa gaaaaagctg ccgaaccacc agcgcgtgca
     6661 cgcggtgcca ttgcgcgcac ctacttctat atgcgcgacc aatacaacct gacactctct
     6721 cgccagcaaa cgcagctgtt caacgcatgg aacaagatgt atccggttac cgactgggag
     6781 tgcgagcgcg atgaacgcat cgcgaaggtg cagggcaatc ataacccgta tgtgcaacgc
     6841 gcttgccagg cgcgaaagag ctaacctaca ctagcgggat tctttttgtt aacccctacc
     6901 ccacgcgtac aaccgcgtgg ggagacgacg cggattttta actatgcgta tcccccgcat
     6961 ttatcatcct gaaccactga ccagccattc tcacatcgcg ctttgcgaag atgccgccaa
     7021 ccatatcggg cgcgtactgc gcatggggcc ggggcaggcg ttgcaattgt ttgacggtag
     7081 caaccaggtc tttgacgccg aaattaccag cgccagcaaa aaaagcgtgg aagtgaaggt
     7141 gctggaaggc cagatcgacg atcgcgaatc tccgctgcat attcacctcg gtcaggtgat
     7201 gtcgcgtggt gaaaaaatgg aatttactat ccagaaatcg atcgaactcg gtgtaagcct
     7261 cattacgcca cttttttctg agcgctgcgg cgttaaactg gatagtgaac gtctgaacaa
     7321 gaagcttcag cagtggcaga agattgcaat tgctgcctgt gagcagtgtg gtcgtaaccg
     7381 ggtgccggaa atccgtccag cgatggatct ggaagcctgg tgtgcagagc aggatgaagg
     7441 actgaaactg aatcttcacc cgcgcgccag taacagcatc aatacgttgc ctttaccggt
     7501 tgaacgcgtc cgcctgctga ttggcccgga aggcggttta tcggcagatg aaattgccat
     7561 gactgcccgc tatcaattta ctgatatcct gttgggacct cgcgttttgc gtacagagac
     7621 aactgcgctc accgccatta ccgcgctaca agtacgattt ggcgatttgg gctaacggag
     7681 aagaataatg atcaagctcg gcatcgtgat ggaccccatc gcaaacatca acatcaagaa
     7741 agattccagt ttcgctatgt tgctggaagc acagcgtcgt ggttacgaac ttcactatat
     7801 ggagatgggc gatctgtatc tgatcaatgg cgaagcccgc gcccataccc gcacgctgaa
     7861 cgtgaagcag aactacgaag agtggttttc gttcgtcggt gaacaggatc tgccgctggc
     7921 cgatctcgat gtgatcctga tgcgtaaaga cccgccgttt gataccgagt ttatctacgc
     7981 gacctatatt ctggaacgtg ccgaagagaa agggacgctg atcgttaaca agccgcagag
     8041 cctgcgcgac tgtaacgaga aactgtttac cgcctggttc tctgacttaa cgccagaaac
     8101 gctggttacg cgcaataaag cgcagctaaa agcgttctgg gagaaacaca gcgacatcat
     8161 tcttaagccg ctggacggta tgggcggcgc gtcgattttc cgcgtgaaag aaggcgatcc
     8221 aaacctcggc gtgattgccg aaaccctgac tgagcatggc acttgctact gcatggcgca
     8281 aaattacctg ccagccatta aagatggcga caaacgcgtg ctggtggtgg atggcgagcc
     8341 ggtaccgtac tgcctggcgc gtattccgca ggggggcgaa acccgtggca atctggctgc
     8401 cggtggtcgc ggtgaacctc gtccgctgac ggaaagtgac tggaaaatcg cccgtcagat
     8461 cgggccgacg ctgaaagaaa aagggctgat ttttgttggt ctggatatca tcggcgaccg
     8521 tctgactgaa attaacgtca ccagcccaac ctgtattcgt gagattgaag cagagtttcc
     8581 ggtgtcgatc accggaatgt taatggatgc catcgaagca cgtttacagc agcagtaacc
     8641 caccttagcg agaaggatct cgttgagact ctgagtgaca gcgcccttct ttccacgcat
     8701 actgggcgct gttgcttttt tgaaccagga aacagaacct ctgacaatga atttacagca
     8761 tcactttctt attgccatgc ctgctctcca ggatccgatt ttccgtcgtt ccgtggtcta
     8821 catttgcgaa cataatacca atggtgcaat ggggatcatc gtcaacaagc cgctggaaaa
     8881 tctcaaaatt gaagggattc tggaaaagct gaagatcacg ccggagccgc gtgatgaatc
     8941 aatccgtctg gataaaccgg ttatgctcgg cggcccactg gctgaagatc gcgggtttat
     9001 tttgcatact ccgccctcca attttgcttc cagcattcgc atttcagaca acacggtgat
     9061 gaccacttcc cgcgatgtgc tggaaacgct cggcaccgat aaacaaccgt ctgacgtatt
     9121 ggtggctctg ggctatgcct cctgggaaaa aggtcaactg gaacaagaaa ttctcgataa
     9181 cgcgtggcta acggccccgg cggatctgaa tattctgttc aaaacgccga ttgccggccg
     9241 ctggcgcgag gcggcaaaac tgattggtgt ggatattctc accatgcctg gtgtggcagg
     9301 acacgcctga tgagtggaac cttactcgcc ttcgacttcg gcaccaaaag cattggcgta
     9361 gcggtcggcc aacgcattac cggcaccgct cgccctttgc ctgcaattaa agcgcaggac
     9421 ggtacgccgg actggaacct catcgaacgt ttactgaaag agtggcagcc tgacgaaatc
     9481 atcgtcggtt tgccgctgaa tatggacggc accgaacagc cgttaaccgc cagagcgcgc
     9541 aaatttgcca accgtattca tggtcgtttc ggtgttgaag taaagctcca tgacgagcgt
     9601 cttagcactg tggaagcccg ttccggtctt tttgaacagg gcggctatcg ggcgctcaac
     9661 aaaggcaaag ttgactctgc ctctgcggtt attattctcg aaagctattt cgagcaggga
     9721 tattaaggcg atctaaaagc ctggcggata gtcgttaccg ccatcttgcc ggatgcggcg
     9781 tgaacgcctt atccagcaca catctggcag cggctgtagg tctgataaga cgcgttagcg
     9841 tcgcatcaga catttattgc ctttataggc cctataaact tgcgcatcgg gcatggcaac
     9901 gtcacaaacg cccttccccc acccgctgct gataactctg ctgaaacgtt aacatcccca
     9961 cctgctgccc ggtttgaata acatgcggta actggtgggt tttcccttcg cgaatcaaat
    10021 tccccaccgc gggagtgtta atcagtaatt caaacagcgc cacgcgtcct tcctgtttat
    10081 ccacttccag cttttgtgac agcactgccc gtaaactacc cgcaagttga ttacgtacgg
    10141 gatctttttc ctgcgccgga aatgaatcca ccagtcgctc aactgcctgc gcggcaccac
    10201 gtgtatgtaa ggttgccagc accagatgcc cggtttctgc cgccgtcagc gccagacgga
    10261 ttgtctcact gtcacgtagc tctccgagca aaatcacatc aggatcttcc cgcaatgcag
    10321 cccgcaaccc cgatgcgaac gtcatacagt gcaaaccaat ttcccgctgc tggatcaaac
    10381 atcgctggct ggcatagaga tattccacag gatcttccag cgtcagaata tgcgcatcgg
    10441 catgttgatt aagatagcca accatcgccg ccagcgtggt agatttgcca ctccccgtag
    10501 cccccgtcac cagaatcagg ccattctcgc tcttgagtaa ttccggcaat accggtggtg
    10561 caccaagctg ttcgagctgt ggacagtgcg aaggtaacaa ccgtaatgcc agagaaatac
    10621 cttgccgttg cgcgaacgca ctgccacgca accgctggtt ttccgccagc gacacggcaa
    10681 aatccagctg gccattttcc agcaatattg cccgctgatc gtcatccagc cactcccgca
    10741 gtagctcttc gacgtccggc gcatcaaacg gcgcagcttc cattctgcca cgaatgcgcc
    10801 atcgtgcggg ccaggcgctg cacaggtgta gatccgagac gttatgcttt acactaaggg
    10861 ccacaatttc ttccatattc atactaagat cctcggaaaa tgaacgatat tgcgcataac
    10921 ctggcacagg tccgggacaa aatctcagcg gctgcaacgc gttgcggccg ttctccagaa
    10981 gaaattacgc tgcttgcagt cagtaaaaca aaacctgcga gcgccatcgc agaagccatt
    11041 gatgccgggc agcgtcaatt tggtgaaaac tacgttcagg aaggggtaga taaaattcgc
    11101 cactttcagg aactgggcgt aacaggatta gaatggcatt ttattggccc gttgcagtct
    11161 aataaaagcc gcctagtggc agagcatttc gactggtgtc ataccatcga ccgtttgcgc
    11221 attgctaccc gcctcaacga gcagcgcccg gcagaacttc cccctcttaa cgtactgatt
    11281 caaattaata ttagtgatga aaacagtaag tccgggattc aactggcaga actggacgag
    11341 ctggcagcgg cggtcgctga actaccgcgt ttacgtctgc gcgggctgat ggcaatccct
    11401 gcgcctgagt cagaatatgt aaggcagttt gaagttgcac gccaaatggc tgtagcattt
    11461 gccggactga aaacgcgcta cccgcatatc gacacgctct ctctgggaat gtcggacgat
    11521 atggaagccg ccattgcggc aggtagcacg atggttcgta tcggcactgc aatttttggt
    11581 gcgcgtgatt actctaaaaa ataaggaatt aaaggaacgc catgaatacg ttgactttcc
    11641 tgctttcaac ggtcattgag ctgtatacaa tggtgctgtt attacgcatc tggatgcagt
    11701 gggctcattg tgatttttac aaccccttct cacagtttgt agtgaaggta gcgcagccaa
    11761 ttatcgggcc actgcgccgc gttattccgg caatgggtcc cattgacagc gcctcgctgc
    11821 tggttgccta tattctcagt tttatcaaag ccattgtgct gtttgaagtg gtgaccttcc
    11881 tgccaatcat ctggattgcc ggtttactga ttctgctgaa aaccatcggc ttgctgattt
    11941 tctgggtcct gctggtgatg gcgattatga gctgggtaag ccagggtcgt agcccgattg
    12001 aatacgtgct gattcagctg gccgatccgc tgctgcgccc gattcgccgc ttgctaccgg
    12061 caatgggtgg gattgatttc tcgccgatga tcctcgttct gctgctgtat gccatcaata
    12121 tgggtgtcgc agaagtatta caggcaaccg gaaacatgct gctgccgggg ctgtggatgg
    12181 cgttatgagt gccgtaacag ttaatgatga cggtctggtt ttacagctct atattcagcc
    12241 gaaagccagc cgtgattcta ttgtcggttt acatggcgac gaagttaaag tcgccattac
    12301 cgcgccgccg gttgacggcc aggccaacag tcatctggtg aagtttctcg gtaagcaatt
    12361 ccgggttgcc aaaagccagg tggtgattga aaaaggcgaa cttggccgcc acaaacaaat
    12421 taaaatcatt aatccgcaac aaatcccgcc agaaatcgcg gcgttaatta attaggtatc
    12481 ctatgcaaaa agttgtcctc gcaaccggca atgccggtaa agtgcgtgag ctggcgtcgc
    12541 tgcttagcga ctttggtctt gatatcgtgg cccaaacaga cctcggcgtt gattccgctg
    12601 aagaaaccgg cctaaccttt atcgaaaacg cgattctgaa agcgcgccat gcggcaaaag
    12661 tgaccggttt accggcaatt gccgacgact ctggtctggc ggtagatgcg ctgggtggcg
    12721 cgccgggtat ttactccgcg cgttattccg gtgaagacgc gaccgatcaa aagaatctgc
    12781 aaaaactgtt ggaaacactg aaagacgtac cggacgacca acgtcaggcg cgtttccact
    12841 gcgtgctggt atatctgcgt cacgcggaag atccgactcc gctggtatgc cacggcagct
    12901 ggccgggcgt gattactcgc gaaccagcgg gcactggtgg ctttggttat gatccaatct
    12961 tcttcgtacc ttccgaaggg aaaaccgccg ccgaactgac ccgcgaagaa aagagcgcca
    13021 tttcccaccg tggtcaggcg ttgaaactgc tgctggacgc tttacgtaat ggttaaatta
    13081 ccgccgctga gtctctacat tcacatcccg tggtgcgtgc agaaatgccc gtactgcgat
    13141 ttcaactctc acgcgttgaa aggagaagtg ccgcacgacg attatgttca gcatctgctt
    13201 tgcgatctgg acaatgatgt ggcttacgct cagggccgtg aagtaaagac aatctttatt
    13261 ggcggtggta cgccgagcct gctttccggc ccggcgatgc aaacgctgct agacggcgtg
    13321 cgtgcgcgtt tgccgctggc agcggatgca gaaattacta tggaagcgaa cccaggcacg
    13381 gtagaagcgg atcgctttgt cgattatcag cgtgctggtg taaaccgcat ctctattggt
    13441 gtgcagagtt ttagcgaaga aaagctgaaa cgacttgggc gtattcatgg cccgcaagaa
    13501 gcgaaacgag cggcaaagct ggcgagcggt ttagggttac gtagctttaa ccttgatttg
    13561 atgcatggac taccggatca atcactggaa gaggcgcttg gcgatctacg ccaggccatt
    13621 gaactgaatc cgccgcatct ttcctggtat caactgacta tcgaacctaa tacgctgttt
    13681 ggctcgcgac cgccggtgct gccggacgat gacgcgctgt gggatatttt cgaacagggg
    13741 catcagttat taaccgcagc gggttatcag caatatgaaa cttccgctta cgccaaaccc
    13801 ggttatcagt gccagcacaa tctcaactac tggcgctttg gtgactacat cggtattggc
    13861 tgcggcgcgc acggcaaagt gaccttcccg gatgggcgca ttctgcgtac cactaaaacg
    13921 cgtcatcctc gtggttttat gcaaggaagg tatctggaaa gccagcgtga tgtcgaagcc
    13981 gcagataagc cgtttgagtt ctttatgaat cgcttccgtc tgctggaggc cgcgccgcgc
    14041 gtggagttta ttgcgtatac cgggctttgc gaagatgtga ttcgcccaca gttagacgag
    14101 gcgattgccc agggttatct caccgaatgt gcggattact ggcagataac ggaacatggt
    14161 aaattattct taaactcact tcttgaatta ttcctcgctg agtaatcatt aatgcgcctg
    14221 cgggcgcatt aatcacttct tttatccccc cgttaattaa cctcggcaga aatatatttc
    14281 ttactgaata aatatcgacg acatttatta tatatattta gtgaatattt actaatcatt
    14341 cattgattat aaatatgtac aacctggcgc acaaatttaa tatttaattt ctgactttat
    14401 ttcaagttgt gataaacata gacaagttgt cagacaggtt aaccaatttg tcgcacaaat
    14461 cactgataag cacaattaga tatcaaccaa tttccatcct tcaacaggga gtttttttat
    14521 gaaaatgagg tcatttaccc gctcattagt ctgcgcatcc ttgctggctc tggttagcac
    14581 tggcgtaaac gccgcagaaa aagtggcgtt aaagttagca tacaacctgg aaagaagtca
    14641 tgttgtacat cagagttttg aagagctggc gaaagaggta aaacagctgt caaaaggtaa
    14701 tatggtgatc cgtatttatc ccagcagcca gatgggtaat gcgcgagaaa ctatggaatt
    14761 gttgcaaaat ggcgcactgg atatgaccaa aggttccgcc agtgatctgg agtcttttga
    14821 taatatttat gctatctata atttgccgtt cttattaagt tgtttttggt gaagttggta
    14881 aagaaattat ggactcaaca aaagacaaag gcttttttgc tttatcagct tatgtagcgg
    14941 gtactcgcag tttctatgcg aaaaaaccga ttacaaagcc agaagatctt aaaggactga
    15001 aaattcgtgt tcagccaagt ccgaccacca tcaaaatgat tgaactgatg ggtggttcac
    15061 ccactccaat ttcatttggc gaagtttata ctgcgatgca gcaaggtgta gtagatggcg
    15121 cagaaaataa cgtgccttcc tggatgcaga ctcgacatat tgaaattgcc aaagtttttt
    15181 ctgaagatga acacgcctca attcctgact ttttggttat ctccaccaaa acatggaata
    15241 aattaacccc agaacagcaa caaatcctcg aaacagcggc gaagaaatcc gaagcttatc
    15301 aacaaaaatt gtgggaaaaa attgacgctg acacacgtgc tcaggcgaaa gctatggggg
    15361 gtgaaattgt aaaagtggat aaagcgccat tccgtaaagc cgtgcaacct ctgtttgacg
    15421 acttcaaaaa agatccaaaa caagctgctt tgttagagaa attcgacaac gctgctcaat
    15481 aatattccgg gccagcctct ggccctgatt ttccaggatg cttacaatga tatttaaccg
    15541 cctgaaactt gcggtagacc gcgtcattgc cgcgttttct gtcgccgtaa tgcttgctct
    15601 ggttgtatgc gtagtctggc aagtatttag ccgctacgta cttaaccagc caagtacatt
    15661 aaccgatgag ctggcacgct ttttgatgat ctgggtagga ttattaggtg ctgcatatac
    15721 cgtcggcgcg cagcgacatc tctctattga tttatttgct ctcgccctga ataagcgcaa
    15781 acaattactt cttagtatag tcattaacgt tctcatttta ggttttgccg gttcggtcat
    15841 tgtgactggt ggcctgaaat taattgataa aacactggca acatcacagg tatctgccgc
    15901 tatgcaaata ccgatgggat acgtatatat cattctgcca ttaagtggag tggtaatgat
    15961 gttttatgcc ttgtgtttta ttaatcaaag cattcaacaa ctgaaacaac ctgcgcagga
    16021 ggccagttaa tgga
//
LOCUS       DAJTQY010000084        15803 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.084, whole genome shotgun sequence.
ACCESSION   DAJTQY010000084 DAJTQY010000000
VERSION     DAJTQY010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15803
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.084"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            <1..293
                     /gene="tuf"
                     /locus_tag="OO349_002938"
     CDS             <1..293
                     /gene="tuf"
                     /locus_tag="OO349_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR5867936.1"
                     /translation="IKPHTKFESEVYILSKDEGGRHTPFFKGYRPQFYFRTTDVTGTI
                     ELPEGVEMVMPGDNIKMVVTLIHPIAMDDGLRFAIREGGRTVGAGVVAKVLG"
     gene            476..670
                     /gene="bfd"
                     /locus_tag="OO349_002939"
     CDS             476..670
                     /gene="bfd"
                     /locus_tag="OO349_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HCR5867937.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            743..1219
                     /gene="bfr"
                     /locus_tag="OO349_002940"
     CDS             743..1219
                     /gene="bfr"
                     /locus_tag="OO349_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HCR5867938.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(1216..1683)
                     /locus_tag="OO349_002941"
     CDS             complement(1216..1683)
                     /locus_tag="OO349_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HCR5867939.1"
                     /translation="MDTTLPFLILYACLSALLFFWDAKHGLLPDRFTCPLLWSGLLFY
                     QVCHPDGLADALWGAIVGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHSWAFL
                     PRLVFLAASFACGAVVIGLLMRGKESLKNPLPFGPFLAAAGFVIGWDSLLIGR"
     gene            2064..2375
                     /gene="rpsJ"
                     /locus_tag="OO349_002942"
     CDS             2064..2375
                     /gene="rpsJ"
                     /locus_tag="OO349_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HCR5867940.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2408..3037
                     /gene="rplC"
                     /locus_tag="OO349_002943"
     CDS             2408..3037
                     /gene="rplC"
                     /locus_tag="OO349_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HCR5867941.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            3048..3653
                     /gene="rplD"
                     /locus_tag="OO349_002944"
     CDS             3048..3653
                     /gene="rplD"
                     /locus_tag="OO349_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HCR5867942.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLQKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3650..3952
                     /gene="rplW"
                     /locus_tag="OO349_002945"
     CDS             3650..3952
                     /gene="rplW"
                     /locus_tag="OO349_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HCR5867943.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3970..4791
                     /gene="rplB"
                     /locus_tag="OO349_002946"
     CDS             3970..4791
                     /gene="rplB"
                     /locus_tag="OO349_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HCR5867944.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4808..5086
                     /gene="rpsS"
                     /locus_tag="OO349_002947"
     CDS             4808..5086
                     /gene="rpsS"
                     /locus_tag="OO349_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HCR5867945.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            5101..5433
                     /gene="rplV"
                     /locus_tag="OO349_002948"
     CDS             5101..5433
                     /gene="rplV"
                     /locus_tag="OO349_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HCR5867946.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VQVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5451..6152
                     /gene="rpsC"
                     /locus_tag="OO349_002949"
     CDS             5451..6152
                     /gene="rpsC"
                     /locus_tag="OO349_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HCR5867947.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            6165..6575
                     /gene="rplP"
                     /locus_tag="OO349_002950"
     CDS             6165..6575
                     /gene="rplP"
                     /locus_tag="OO349_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HCR5867948.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6575..6766
                     /gene="rpmC"
                     /locus_tag="OO349_002951"
     CDS             6575..6766
                     /gene="rpmC"
                     /locus_tag="OO349_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HCR5867949.1"
                     /translation="MKAKELREKSIEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6766..7020
                     /gene="rpsQ"
                     /locus_tag="OO349_002952"
     CDS             6766..7020
                     /gene="rpsQ"
                     /locus_tag="OO349_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HCR5867950.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            7185..7556
                     /gene="rplN"
                     /locus_tag="OO349_002953"
     CDS             7185..7556
                     /gene="rplN"
                     /locus_tag="OO349_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HCR5867951.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7567..7881
                     /gene="rplX"
                     /locus_tag="OO349_002954"
     CDS             7567..7881
                     /gene="rplX"
                     /locus_tag="OO349_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HCR5867952.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7896..8435
                     /gene="rplE"
                     /locus_tag="OO349_002955"
     CDS             7896..8435
                     /gene="rplE"
                     /locus_tag="OO349_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HCR5867953.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8450..8755
                     /gene="rpsN"
                     /locus_tag="OO349_002956"
     CDS             8450..8755
                     /gene="rpsN"
                     /locus_tag="OO349_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HCR5867954.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8789..9181
                     /gene="rpsH"
                     /locus_tag="OO349_002957"
     CDS             8789..9181
                     /gene="rpsH"
                     /locus_tag="OO349_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HCR5867955.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            9194..9727
                     /gene="rplF"
                     /locus_tag="OO349_002958"
     CDS             9194..9727
                     /gene="rplF"
                     /locus_tag="OO349_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HCR5867956.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9737..10090
                     /gene="rplR"
                     /locus_tag="OO349_002959"
     CDS             9737..10090
                     /gene="rplR"
                     /locus_tag="OO349_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HCR5867957.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            10105..10608
                     /gene="rpsE"
                     /locus_tag="OO349_002960"
     CDS             10105..10608
                     /gene="rpsE"
                     /locus_tag="OO349_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HCR5867958.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10612..10791
                     /gene="rpmD"
                     /locus_tag="OO349_002961"
     CDS             10612..10791
                     /gene="rpmD"
                     /locus_tag="OO349_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HCR5867959.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10795..11229
                     /gene="rplO"
                     /locus_tag="OO349_002962"
     CDS             10795..11229
                     /gene="rplO"
                     /locus_tag="OO349_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HCR5867960.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            11237..12568
                     /gene="secY"
                     /locus_tag="OO349_002963"
     CDS             11237..12568
                     /gene="secY"
                     /locus_tag="OO349_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HCR5867961.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12600..12716
                     /gene="rpmJ"
                     /locus_tag="OO349_002964"
     CDS             12600..12716
                     /gene="rpmJ"
                     /locus_tag="OO349_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HCR5867962.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12863..13219
                     /gene="rpsM"
                     /locus_tag="OO349_002965"
     CDS             12863..13219
                     /gene="rpsM"
                     /locus_tag="OO349_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HCR5867963.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            13236..13625
                     /gene="rpsK"
                     /locus_tag="OO349_002966"
     CDS             13236..13625
                     /gene="rpsK"
                     /locus_tag="OO349_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HCR5867964.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13659..14279
                     /gene="rpsD"
                     /locus_tag="OO349_002967"
     CDS             13659..14279
                     /gene="rpsD"
                     /locus_tag="OO349_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HCR5867965.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14305..15294
                     /gene="rpoA"
                     /locus_tag="OO349_002968"
     CDS             14305..15294
                     /gene="rpoA"
                     /locus_tag="OO349_002968"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HCR5867966.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15335..15718
                     /gene="rplQ"
                     /locus_tag="OO349_002969"
     CDS             15335..15718
                     /gene="rplQ"
                     /locus_tag="OO349_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HCR5867967.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
BASE COUNT         4070 a         3721 c         4204 g         3808 t
ORIGIN      
        1 ccatcaagcc gcacaccaag ttcgaatctg aagtgtacat tctgtccaaa gatgaaggcg
       61 gccgtcatac tccgttcttc aaaggctacc gtccgcagtt ctacttccgt actactgacg
      121 tgactggtac catcgaactg ccggaaggcg tagagatggt aatgccgggc gacaacatca
      181 aaatggttgt taccctgatc cacccgatcg cgatggacga cggtctgcgt ttcgcaatcc
      241 gtgaaggcgg ccgtaccgtt ggcgcgggcg ttgttgctaa agttctgggc taattacacg
      301 ttaattagtt ttgaattgaa aagggcgctt cggcgccctt tttgctgccc gtaatttgag
      361 ctcgaaatat ttagtcgtaa ttttgctcgc tgaactatgg cgcaaataaa actgtactat
      421 tgtaatcata accattctca tttacacttt gtgcagaatt tgaacgggag caatcatgta
      481 cgtttgtttg tgtaatggtg taagtgacaa aaaaattcgc caggcagtcc gtcagtttca
      541 tccgcaatct ttccaacaat tgcgtaagtt tattcctgtg ggaaatcaat gcggtaagtg
      601 cattcgcgcc gcgcgcgaag tgatgcagga cgagttgatg cagatgccag aatttaaaga
      661 gattgcctga agcccacgct tattttttga catcctgcta gccccatcta cgctctaata
      721 gtggaagcgg agggactata aaatgaaagg tgatgttaaa atcataaatt atctcaataa
      781 actattggga aatgagcttg tcgcaatcaa tcagtatttt ctccatgccc ggatgtttaa
      841 aaactggggc ctgatgcgtc tcaatgacgt cgaataccat gaatccatcg atgagatgaa
      901 acacgctgat aaatatatcg agcgtatttt gtttttagaa ggaatcccca acttacagga
      961 tttaggcaaa ctggggattg gtgaagatgt cgaggagatg ctgcaatctg acctgcgatt
     1021 agaactggaa ggcgcgaagg atctgcgtga ggctatcgca tatgccgaca gcgttcatga
     1081 ctatgttagc cgcgatatga tgatcgagat ccttgctgat gaagaaggtc atatcgactg
     1141 gctcgaaact gaactggatc tcatcggtaa aatcggccta caaaattacc tgcaatcaca
     1201 aattaaagtt aaagattaac ggcctatcag caaactatcc cagcctataa cgaaacccgc
     1261 agccgccaga aatggcccaa aaggcagcgg gttttttaat gattcttttc ctctcatgag
     1321 cagaccaata actacggctc cgcaagcgaa tgaggcagca aggaaaacca atcgtggtaa
     1381 gaaggcccat gaatgccagg cgccaagggc ggcgagaaac ttcacatcac catagcctaa
     1441 tccttcttta tgtcgcagta tgcgataccc ccagtaaatg acagcgaatg tgccgtaacc
     1501 aacaattgcc ccccataacg catcggctaa accatcagga tgacaaactt gatagaacaa
     1561 aagacctgac cagagtaacg ggcacgtaaa tctgtcaggt aacaagccat gctttgcatc
     1621 ccagaaaaaa agtagggctg ataaacaagc gtacaaaatt agaaagggaa gggtagtgtc
     1681 cataaaacat cctctgtaat tggagtatca ccatcgaata actccggatg ctcagcaaca
     1741 gagagagagc gctgatttgt taacctgatt ccggtgaaaa acgctatctt cacaacctta
     1801 caaatttcgt ggaatgcagc tcgcaatctg cgcgttgcga ttaatttcta cccgatgctt
     1861 gcgtcaggct cagatttgcg tataatgcgc gggcttgtcg tagttgacag caggttcaat
     1921 ctgaacctca gttagccgaa tttggctacc taacaatgct cccaatcggg gagctacgta
     1981 agaacggtta cactctccca tcaatcgtaa tgggtctgag gagtaatcat tttcgtttat
     2041 aaaataattg gagctctggt ctcatgcaga accaaagaat ccgtatccgc ctgaaagcgt
     2101 ttgatcatcg tctgatcgat caagcaaccg cggaaatcgt cgagactgcc aagcgcactg
     2161 gtgcgcaggt ccgtggtccg atcccgctgc cgacacgcaa agagcgcttc actgttctga
     2221 tctccccgca cgtcaacaaa gacgcgcgcg atcagtacga aatccgtact cacttgcgtc
     2281 tggttgacat cgttgagcca accgagaaaa ccgttgatgc tctgatgcgt ctggatctgg
     2341 ctgccggtgt agacgtgcag atcagcctgg gttaatcagg tcattgagcg attgagaggt
     2401 tgaaacaatg attggtttag tcggtaaaaa agtgggtatg acccgtatct tcacagaaga
     2461 cggcgtttct atcccagtaa ccgtaatcga agttgaagca aaccgcgtta ctcaggttaa
     2521 agacctggct aacgatggct accgtgctat tcaggtgacc accggtgcta aaaaagctaa
     2581 ccgtgtgacc aagcctgaag ctggccactt cgctaaagct ggcgtagaag ctggccgtgg
     2641 tctgtgggaa ttccgcctgg ctgaaggcga agagttcact gtaggtcaga gcattagcgt
     2701 tgaactgttt gctgacgtta aaaaagttga cgtaactggc acctctaaag gtaaaggttt
     2761 cgcaggtacc gttaagcgct ggaacttccg tacccaggac gctactcacg gtaactcctt
     2821 gtctcaccgc gttccgggtt ctatcggtca gaaccagact ccgggcaaag tgttcaaagg
     2881 caagaaaatg gcaggtcaga tgggtaacga acgtgtaacc gttcagagcc ttgacgtagt
     2941 acgcgttgac gctgagcgca acctgctgct ggttaaaggt gctgtcccgg gtgcaaccgg
     3001 tagcgacctg atcgttaaac cagctgtgaa ggcgtaagga gatagcaatg gaattagtat
     3061 tgaaagacgc gcagagcgcg ctgactgttt ccgaaactac cttcggtcgt gatttcaacg
     3121 aagcgctggt tcaccaggtt gttgttgctt atgcagctgg tgctcgtcag ggtactcgtg
     3181 ctcagaagac tcgtgctgaa gtaactggtt ccggtaaaaa accgtggcgc cagaaaggca
     3241 ccggccgtgc gcgttctggt tctatcaaga gcccgatctg gcgttctggt ggcgtgacct
     3301 ttgctgctcg tccgcaggac cacagtcaaa aagttaacaa gaagatgtac cgcggcgcgc
     3361 tgaaaagcat cctgtccgaa ctggtacgtc aggatcgtct gatcgttgtc gagaagttct
     3421 ctgtagaagc gccgaaaact aagctgctgg cacagaaact gaaagacatg gctctggaag
     3481 atgtgctgat catcaccggt gagctggacg aaaacctgtt cctggctgcg cgcaacctgc
     3541 agaaggttga cgtacgcgat gcaactggta tcgacccggt tagcctgatc gccttcgaca
     3601 aagtcgtaat gactgctgat gctgttaagc aagttgagga gatgctggca tgattcgtga
     3661 agaacgtctg ctgaaggtgc tgcgtgcacc gcacgtttct gaaaaagcgt ctactgcgat
     3721 ggaaaaatcc aacaccatcg tactcaaagt tgcgaaagac gcgaccaaag cagaaatcaa
     3781 agctgctgtg cagaaactgt ttgaagtcga agtcgaagtc gttaacaccc tggtagttaa
     3841 agggaaagtt aaacgtcacg gacagcgtat cggtcgtcgt agcgactgga aaaaagctta
     3901 cgtcaccctg aaagaaggcc agaatctgga cttcgttggc ggcgctgagt aagtcggagg
     3961 agtaatacaa tggcagttgt taaatgtaaa ccgacatctc cgggtcgtcg ccacgtagtt
     4021 aaagtggtta accctgagct gcacaagggc aaaccttttg ctccgttgct ggaaaaaaac
     4081 agcaaatccg gtggtcgtaa caacaatggc cgtatcacca ctcgtcatat cggtggtggc
     4141 cacaagcagg cttaccgtat tgttgacttc aaacgcaaca aagacggtat cccggcagtt
     4201 gttgaacgtc ttgagtacga tccgaaccgt tccgcgaaca tcgcgctggt tctgtacaaa
     4261 gacggtgaac gccgttacat cctggcccct aaaggcctga aagctggcga ccagattcag
     4321 tctggcgttg atgctgcaat caaaccaggt aacaccctgc cgatgcgcaa catcccggtt
     4381 ggttctactg ttcataacgt agaaatgaaa ccaggtaaag gcggtcagct ggcacgttcc
     4441 gctggtactt acgttcagat cgttgctcgt gatggtgctt atgtcaccct gcgtctgcgt
     4501 tctggtgaaa tgcgtaaagt agaagcagac tgccgtgcaa ctctgggcga agttggcaat
     4561 gctgagcata tgctgcgcgt tctgggtaaa gcaggtgctg cacgctggcg tggtgttcgt
     4621 ccgaccgttc gcggtaccgc gatgaacccg gtagaccacc cacatggtgg tggtgaaggt
     4681 cgtaactttg gtaagcaccc ggtaactccg tggggcgttc agaccaaagg taagaagacc
     4741 cgcagcaaca agcgtactga taaattcatc gtacgtcgcc gtagcaaata attttagagg
     4801 ataagccatg ccacgttctc tcaagaaagg tccttttatt gacctgcact tgctgaagaa
     4861 ggtagagaaa gcggtggaaa gcggagacaa gaagcccctg cgcacttggt cccgtcgttc
     4921 aacgatcttt cctaacatga tcggtttgac catcgctgtc cataatggtc gtcagcacgt
     4981 tccggtattt gtaaccgacg aaatggtcgg tcacaaactg ggtgaattcg caccgactcg
     5041 tacttatcgc ggccacgctg ctgataaaaa agcgaagaag aaataaggta ggaggaagag
     5101 atggaaacta ttgctaaaca tcgccatgct cgttcttctg ctcagaaggt tcgccttgtt
     5161 gctgacctga ttcgcggtaa gaaagtgtcg caggctctgg atattttgac ctacaccaac
     5221 aagaaagcgg ctgtacaggt caagaaagtt ctggaatctg ccattgctaa cgctgaacac
     5281 aacgatggcg ctgacattga cgatctgaaa gttacgaaaa ttttcgtaga cgaaggcccg
     5341 agcatgaagc gcattatgcc gcgtgcaaaa ggtcgtgcag atcgcatcct gaagcgcacc
     5401 agccacatca ctgtggttgt gtccgatcgc tgagactctg gagactagca atgggtcaga
     5461 aagtacatcc taatggtatt cgcttgggta ttgtaaaacc atggaactct acttggtttg
     5521 cgaacaccaa agaattcgct gacaacctgg acagcgattt taaagtacgt cagtacctga
     5581 ctaaggaact ggctaaagcg tccgtatctc gtatcgttat cgagcgtccg gctaagagca
     5641 tccgtgtaac cattcacact gctcgcccgg gtatcgttat cggtaaaaaa ggtgaagacg
     5701 tagaaaaact gcgtaaggtc gtagcggaca tcgctggcgt tcctgcacag atcaacatcg
     5761 ccgaagttcg taagcctgaa ctggacgcaa aactggttgc tgacagcatc acttctcagc
     5821 tggaacgtcg cgttatgttc cgtcgtgcta tgaagcgtgc tgtacagaac gcaatgcgtc
     5881 tgggcgctaa aggtattaaa gttgaagtta gcggccgtct gggcggcgcg gaaatcgcac
     5941 gtaccgaatg gtaccgcgaa ggtcgcgtac cgctgcacac tctgcgtgct gacatcgact
     6001 acaacacctc tgaagcgcac accacttacg gtgtaatcgg cgttaaagtg tggatcttca
     6061 aaggcgagat cctgggtggt atggctgctg ttgaacaacc ggaaaaaccg gctgctcagc
     6121 ctaaaaagca gcagcgtaaa ggccgtaaat aaggagcgtc gctgatgtta caaccaaagc
     6181 gtacaaaatt ccgtaaaatg cacaaaggcc gtaaccgcgg tctggcgcag ggtacggatg
     6241 ttagcttcgg cagcttcggt ctgaaagctg ttggccgtgg tcgtctgact gcccgtcaga
     6301 tcgaagcagc acgtcgtgct atgacccgtg cagttaagcg tcaaggtaag atctggatcc
     6361 gtgtgttccc ggacaaaccg atcactgaaa agccgctggc agtgcgtatg ggtaaaggta
     6421 aaggtaacgt ggagtattgg gttgccttga ttcagccggg taaagtcctg tatgaaatgg
     6481 acggtgttcc ggaagagctg gcccgtgaag cattcaagct ggcagcagcg aaactgccga
     6541 ttaaaaccac ctttgtaact aagacggtga tgtaatgaaa gcaaaagagc tgcgtgagaa
     6601 gagcattgaa gagctgaaca ccgagctgct gaacctgctg cgtgagcagt tcaacctgcg
     6661 tatgcaggct gcaagtggcc agctgcaaca gtctcacctg ttgaagcaag tgcgtcgcga
     6721 tgtcgcacgc gttaagactt tactgaacga gaaggcgggt gcgtaatgac cgataaaatc
     6781 cgtactctgc aaggtcgcgt tgttagcgac aaaatggaga aatccattgt tgttgctatc
     6841 gaacgttttg tgaaacaccc gatctacggt aaattcatca agcgtacgac caaactgcac
     6901 gtacatgacg agaacaacga atgcggtatc ggtgacgtgg ttgaaatccg cgaatgccgt
     6961 ccgctgtcca agactaaatc ctggacgctg gttcgcgttg tagagaaagc ggttctgtaa
     7021 tacagtacac tctctcgata cgaataaacg gctcagaaat gagccgttta ttttttctac
     7081 ccatatcctt gaagcggtgt tataatgccg cgccctcgat atggggattt ttaacgacct
     7141 gattttcggg tctcagtagt agttgacatt agcggagcac taaaatgatc caagaacaga
     7201 ctatgctgaa cgtcgccgac aactccggtg cacgtcgcgt aatgtgtatc aaggttctgg
     7261 gtggctcgca ccgtcgctac gcaggcgtag gcgacatcat caagatcacc atcaaagaag
     7321 caattccgcg tggtaaggtc aaaaaaggtg atgtgctgaa ggcggtagtg gtgcgcacca
     7381 agaagggtgt tcgtcgcccg gacggttctg tcattcgctt cgatggtaat gcttgtgttc
     7441 ttctgaacaa caacagcgag cagcctatcg gtacgcgtat ttttgggccg gtaactcgtg
     7501 agcttcgtag tgagaagttc atgaaaatta tctctctggc accagaagta ctctaaggag
     7561 cgaatcatgg cagcgaaaat ccgtcgtgat gacgaagtta tcgtgttaac cggtaaagat
     7621 aaaggtaaac gcggtaaagt taagaatgtc ctgtcttccg gcaaggtcat tgttgaaggt
     7681 atcaacctgg ttaagaaaca tcagaagccg gttccggccc tgaaccaacc gggtggcatc
     7741 gttgaaaaag aagccgctat tcaggtttcc aacgtagcaa tcttcaatgc ggcaaccggc
     7801 aaggctgacc gtgtaggctt tagattcgaa gacggcaaaa aagtccgttt cttcaagtct
     7861 aacagcgaaa ctatcaagta atttggagta gtacgatggc gaaactgcat gattactaca
     7921 aagacgaagt agttaaaaaa ctcatgactg agtttaacta caattctgtc atgcaagtcc
     7981 ctcgggtcga gaagatcacc ctgaacatgg gtgttggtga agcgatcgct gacaaaaaac
     8041 tgctggataa cgcagcagca gacctggcag caatctccgg tcaaaaaccg ctgatcacca
     8101 aagcacgcaa atctgttgca ggcttcaaaa tccgtcaggg ctatccgatc ggctgtaaag
     8161 taactctgcg tggcgaacgc atgtgggagt tctttgagcg cctgatcact attgctgtac
     8221 ctcgtatccg tgacttccgt ggcctgtccg ctaagtcttt cgacggtcgt ggtaactaca
     8281 gcatgggtgt ccgtgagcag atcatcttcc cagaaatcga ctacgataaa gtcgaccgcg
     8341 ttcgtggttt ggatattacc attaccacta ctgcgaaatc tgacgaagaa ggccgcgctc
     8401 tgctggctgc ctttgacttc ccgttccgca agtaaggtag ggttactaaa tggctaagca
     8461 atcaatgaaa gcacgcgaag taaaacgcgt agctttagct gataaatact tcgcgaaacg
     8521 cgctgaactg aaagcgatca tctctgatgt gaacgcttcc gacgaagatc gttggaacgc
     8581 tgttctcaag ctgcagactc tgccgcgtga ttccagcccg tctcgtcagc gtaaccgctg
     8641 ccgtcaaaca ggtcgtccgc atggtttcct gcggaagttc gggttgagcc gtattaaggt
     8701 ccgtgaagcc gctatgcgcg gtgaaatccc gggtctgaaa aaggctagct ggtaattgtc
     8761 accaattgaa tcacgggagt aaagacagat gagcatgcaa gatccgatcg cggatatgct
     8821 gacccgtatc cgtaacggtc aggccgcgaa caaagctgcg gtcaccatgc cttcctccaa
     8881 gctgaaagtg gcaatcgcca acgtgctgaa ggaagaaggt tttattgaag attttaaagt
     8941 tgaaggcgac accaagcctg aactggaact tactctgaag tatttccagg gcaaagctgt
     9001 tgtagaaagc attcagcgtg tcagccgccc aggtctgcgc atctataaac gtaaagatga
     9061 gctgccgaaa gttatggcgg gtctgggtat cgcagttgtt tctacctcta aaggtgttat
     9121 gactgatcgt gcagcgcgcc aggctggtct tggtggcgaa attatctgct acgtagccta
     9181 atcggaggaa aaaatgtctc gtgttgctaa agcaccggtc gttgttcctg ccggcgttga
     9241 cgtaaaaatc aacggtcagg ttattacgat caaaggtaaa aacggcgagc tgactcgtac
     9301 tctcaacgat gctgttgaag ttaaacatgc agataatacc ctgaccttcg gtccgcgtga
     9361 tggttacgca gacggttggg cacaggctgg taccgcgcgt gccctgctga actcaatggt
     9421 tatcggtgtt accgaaggct tcactaagaa gctgcagctg gttggtgtag gttaccgtgc
     9481 agcggttaaa ggcaatgtga ttaacctgtc tctgggtttc tctcatcctg ttgaccatca
     9541 gctgcctgcg ggtatcactg ctgaatgtcc gactcagact gaaatcgtgc tgaaaggcgc
     9601 tgataagcag gtgatcggcc aggttgcagc ggatctgcgc gcctaccgtc gtcctgagcc
     9661 ttacaaaggc aagggtgttc gttacgccga cgaagtcgtg cgtaccaaag aggctaagaa
     9721 gaagtaaggt aacactatgg ataagaaatc tgctcgtatc cgtcgtgcga cccgcgcacg
     9781 ccgcaagctc caggagctgg gcgcaactcg cctggtggta catcgtaccc cgcgtcacat
     9841 ttacgcacag gtaattgcac cgaacggttc tgaagttctg gtagctgctt ctactgtaga
     9901 aaaagctatc gctgaacaac tgaagtacac cggtaacaaa gacgcggctg cagctgtggg
     9961 taaagctgtc gctgaacgcg ctctggaaaa aggcatcaaa gatgtatcct ttgaccgttc
    10021 cgggttccaa tatcatggtc gtgtccaggc actggcagat gctgcccgtg aagctggcct
    10081 tcagttctaa ggtagaggtg taagatggct cacatcgaaa aacaagctgg cgaactgcag
    10141 gaaaagctga tcgcggtaaa ccgcgtatct aaaaccgtta aaggtggtcg tattttctcc
    10201 ttcacagctc tgactgtagt tggcgatggt aacggtcgcg ttggttttgg ttacggtaaa
    10261 gcgcgtgaag ttccagcagc gatccagaaa gcgatggaaa aagcccgtcg caatatgatt
    10321 aacgtcgcgc tgaataacgg cactctacaa caccctgtta aaggtgttca cacgggttct
    10381 cgcgtattca tgcagccggc ttccgaaggt accggtatca tcgccggtgg tgcaatgcgc
    10441 gccgttctgg aagtcgctgg ggttcataac gttctggcta aagcatatgg ttccaccaac
    10501 ccgatcaacg tggttcgtgc aactattgat ggcctggaaa atatgaattc tccagaaatg
    10561 gtcgctgcca agcgtggtaa atccgttgaa gaaattctgg ggaaataaac catggcaaag
    10621 actattaaaa ttactcaaac ccgcagtgca atcggtcgtc tgccgaaaca caaggcaacg
    10681 ctgcttggct tgggtctgcg tcgtattggt cacaccgtag agcgcgagga tactcctgct
    10741 attcgcggta tgatcaacgc ggtttccttc atggttaaag ttgaggagta agagatgcgt
    10801 ttaaatactc tgtctccggc cgaaggctcc aaaaaggcgg gtaaacgcct gggtcgtggt
    10861 atcggttctg gcctcggtaa aaccggtggt cgtggtcaca aaggtcagaa gtctcgttct
    10921 ggcggtggcg tacgtcgcgg tttcgagggt ggtcagatgc ctctgtaccg tcgtctgccg
    10981 aaattcggct tcacttctcg taaagcagcg attacagccg aagttcgtct atctgacctg
    11041 gctaaagtag aaggcggtgt tgtagacctg aacacgctga aagcggctaa cattatcggt
    11101 atccagatcg agttcgcgaa agtgatcctg gctggcgaag taacgactcc ggtaactgtt
    11161 cgtggcctgc gtgttactaa aggcgctcgt gctgctatcg aagctgctgg cggtaaaatc
    11221 gaggaataag tagcagatgg ctaaacaacc gggattagat tttcaaagtg ccaaaggtgg
    11281 cttaggcgag ctgaaacgca gactgctgtt tgttatcggt gcgctgattg tgttccgtat
    11341 tggctctttt attccgatcc ctggtattga tgccgctgta cttgccaaac tgcttgagca
    11401 acagcgaggc accatcattg agatgtttaa catgttctct ggtggtgctc tcagccgtgc
    11461 ttctatcttt gctctgggga tcatgccgta tatttcggcg tcgatcatta tccagctgct
    11521 gacggtggtt cacccaacgt tggcagaaat taagaaagaa ggggagtctg gtcgtcgtaa
    11581 gatcagccag tacacccgtt acggtactct ggtgctggca atattccagt cgatcggtat
    11641 tgctaccggt ctgccgaata tgcctggtat gcaaggcttg gtgattaacc cgggctttgc
    11701 attctacttc accgctgttg taagtctggt cacaggaacg atgttcctga tgtggttggg
    11761 cgaacagatc actgaacgag gtatcggcaa cggtatttca atcattatct tcgccggtat
    11821 tgtcgcggga ctcccgccag ccattgccca tactatcgag caagcgcgtc aaggcgacct
    11881 gcacttcctc gtgttgctgt tggttgcagt attagtattt gcagtgacgt tctttgttgt
    11941 gtttgttgag cgtggtcaac gccgcattgt ggtaaactac gcgaaacgtc agcaaggtcg
    12001 tcgtgtctat gctgcacaga gcacacattt accgctgaaa gtgaatatgg cgggggtaat
    12061 cccggcaatc ttcgcttcca gtattattct gttcccggcg accatcgcgt catggttcgg
    12121 gggcggtact ggttggaact ggctgacaac aatttcgctg tatttgcagc ctgggcaacc
    12181 gctttatgtg ttactctatg cgtctgcaat catcttcttc tgtttcttct acacggcgtt
    12241 ggttttcaac ccgcgtgaaa cagcagataa cctgaagaag tccggtgcat ttgtaccagg
    12301 aattcgtccg ggagagcaaa cggcgaagta tatcgataaa gtaatgaccc gcctgacctt
    12361 ggttggtgcg ctgtatatta cctttatctg cctgatcccg gagttcatgc gtgatgcaat
    12421 gaaagtaccg ttctacttcg gtgggacctc actgcttatc gttgttgtcg tgattatgga
    12481 ctttatggct caagtgcaaa ctctgatgat gtccagtcag tatgagtctg cattgaagaa
    12541 ggcgaacctg aaaggctacg gccgataatt ggtcgcccga gaagttacgg agagtaaaaa
    12601 tgaaagttcg tgcttccgtc aagaaattat gccgtaactg caaaatcgtt aagcgtgatg
    12661 gtgtcatccg tgtgatttgc agtgccgagc cgaagcataa acagcgccaa ggctgatttt
    12721 ttcgcatatt tttcttgcaa agttgggttg agctggctag attagccagc caatcttttg
    12781 tatgtctgta cgtttccatt tgagtatcct gaaaacgggc ttttcagcat ggaacgtaca
    12841 tattaaatag taggagtgca tagtggcccg tatagcaggc attaacattc ctgatcataa
    12901 gcatgccgta atcgcattaa cttcgattta tggcgtcggc aagacccgtt ctaaagccat
    12961 cctggctgca gcgggtatcg ctgaagatgt taagatcagt gagctgtctg aaggacaaat
    13021 cgacacgctg cgtgacgaag ttgccaaatt tgtcgttgaa ggtgatctgc gccgtgaaat
    13081 cagcatgagc atcaagcgcc tgatggatct tggttgctat cgcggtttgc gtcatcgtcg
    13141 tggtctcccg gttcgcggtc agcgtaccaa gaccaacgca cgtacccgta agggtccgcg
    13201 caaaccgatc aagaaataat cggggtgatt gaataatggc aaaggcacca attcgtgcac
    13261 gtaaacgtgt aagaaaacaa gtctctgacg gcgtggctca tatccatgct tctttcaaca
    13321 acaccatcgt gactatcact gatcgtcagg gtaacgcgtt gggttgggca acagccggtg
    13381 gttccggttt ccgtggttct cgcaaatcca ctccgtttgc agctcaggtt gcagcagagc
    13441 gttgcgctga cgccgtgaaa gaatacggca tcaagaatct ggaagttatg gttaaaggtc
    13501 cgggtccagg ccgcgaatct actattcgtg ctctgaacgc cgcaggtttc cgcatcacta
    13561 acattactga tgtgactccg atccctcata acggttgtcg tccgccgaaa aaacgtcgcg
    13621 tataacgcct cgttttccag gtttgttgga gaaagaaaat ggcaagatat ttgggtccta
    13681 agctcaagct gagccgtcgt gagggcaccg acttattcct taagtctggc gttcgcgcga
    13741 tcgataccaa gtgtaaaatt gaacaagctc ctggccagca cggtgcgcgt aaaccgcgtc
    13801 tgtctgacta tggtgtgcag ttgcgtgaaa agcaaaaagt tcgccgtatc tatggtgtgc
    13861 tggagcgtca gttccgtaac tactacaaag aagcagcacg tctgaaaggc aacaccggtg
    13921 aaaacctgtt ggctctgctg gaaggtcgtc tggacaacgt tgtataccgt atgggcttcg
    13981 gtgccactcg tgcagaagca cgtcagctgg ttagccataa agcaattatg gtaaacggtc
    14041 gtgttgttaa catcgcttct tatcaggtta gtccgaatga cgttgtaagc attcgtgaga
    14101 aagcgaagaa gcagtctcgc gtgaaagccg ctctggagct ggctgagcag cgtgaaaagc
    14161 caacttggct ggaagttgat gctggcaaga tggaaggtac gtttaagcgt aagccggagc
    14221 gttctgatct gtctgcggac attaacgaac acctgatcgt cgagctttac tccaagtaaa
    14281 gcttagtacc aaagagagga cacaatgcag ggttctgtga cagagtttct aaaaccgcgc
    14341 ctggttgata tcgagcaagt gagttcgacg cacgccaagg tgacccttga gcctttagag
    14401 cgtggctttg gccatactct gggtaacgca ctgcgccgta ttctgctctc atcgatgccg
    14461 ggttgcgcgg tgaccgaggt tgagattgat ggtgtactac atgagtacag caccaaagaa
    14521 ggcgttcagg aagatatcct ggaaatcctg ctcaacctga aagggctggc ggtgagagtt
    14581 cagggcaaag atgaagttat tcttaccttg aataaatctg gcattggccc tgtgactgca
    14641 gccgatatca cccacgacgg tgatgtcgaa atcgtcaagc cgcagcacgt gatctgccac
    14701 ctgaccgatg agaacgcgtc tattagcatg cgtatcaaag ttcagcgcgg tcgtggttat
    14761 gtgccggctt ctacccgaat tcattcggaa gaagatgagc gcccaatcgg ccgtctgctg
    14821 gtcgacgcat gctacagccc tgtggagcgt attgcctaca atgttgaagc agcgcgtgta
    14881 gaacagcgta ccgacctgga caagctggtc atcgaaatgg aaaccaacgg cacaatcgat
    14941 cctgaagagg cgattcgtcg tgcggcaacc attctggctg aacaactgga agctttcgtt
    15001 gacttacgtg atgtacgtca gcctgaagtg aaagaagaga aaccagagtt cgatccgatc
    15061 ctgctgcgcc ctgttgacga tctggaattg actgtccgct ctgctaactg ccttaaagca
    15121 gaagctatcc actatatcgg tgatctggta cagcgtaccg aggttgagct ccttaaaacg
    15181 cctaaccttg gtaaaaaatc tcttactgag attaaagacg tgctggcttc ccgtggactg
    15241 tctctgggca tgcgcttgga aaactggcca ccggcaagca tcgctgacga gtaaccggat
    15301 cacaggttaa ggttttactg agaaggataa ggtcatgcgc catcgtaaga gtggtcgtca
    15361 actgaaccgc aacagcagcc atcgccaggc tatgttccgc aatatggcag gttcactggt
    15421 tcgtcatgaa atcatcaaga cgactctgcc taaagcgaaa gagctgcgcc gcgtagttga
    15481 gccgctgatt actcttgcca agactgatag cgttgctaat cgtcgtctgg cattcgcccg
    15541 tactcgtgat aacgagatcg tggcaaaact gtttaacgaa ctgggcccgc gtttcgcgag
    15601 ccgtgccggt ggttacactc gtattctgaa gtgtggcttc cgtgcaggcg acaacgcgcc
    15661 gatggcttac atcgagctgg ttgatcgttc agagaaagca gaagctgctg cagagtaatc
    15721 tgaagcaatg taaaaaaacc cgccccggcg ggttttttta tacccgtagt atccccactt
    15781 atctacaata gctgtactct ttt
//
LOCUS       DAJTQY010000085        15481 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.085, whole genome shotgun sequence.
ACCESSION   DAJTQY010000085 DAJTQY010000000
VERSION     DAJTQY010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15481
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.085"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(<1..73)
                     /locus_tag="OO349_002970"
     CDS             complement(<1..73)
                     /locus_tag="OO349_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="MliC; membrane-bound lysozyme inhibitor of c-type
                     lysozyme; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor"
                     /protein_id="HCR5867968.1"
                     /translation="MTMKKLLIIILPVLLSGCSAFNQL"
     gene            complement(171..1280)
                     /gene="anmK"
                     /locus_tag="OO349_002971"
     CDS             complement(171..1280)
                     /gene="anmK"
                     /locus_tag="OO349_002971"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HCR5867969.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAVLLLGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANS"
     gene            1554..2021
                     /gene="slyB"
                     /locus_tag="OO349_002972"
     CDS             1554..2021
                     /gene="slyB"
                     /locus_tag="OO349_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HCR5867970.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            complement(2068..2502)
                     /gene="slyA"
                     /locus_tag="OO349_002973"
     CDS             complement(2068..2502)
                     /gene="slyA"
                     /locus_tag="OO349_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HCR5867971.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            2703..2939
                     /locus_tag="OO349_002974"
     CDS             2703..2939
                     /locus_tag="OO349_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HCR5867972.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            2942..3799
                     /locus_tag="OO349_002975"
     CDS             2942..3799
                     /locus_tag="OO349_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR5867973.1"
                     /translation="MSIKTIKYFSTIIVAIVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGELLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFIFAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQLVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            3799..5796
                     /locus_tag="OO349_002976"
                     /pseudo
     CDS             3799..5796
                     /locus_tag="OO349_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
     gene            complement(5797..6318)
                     /gene="sodC"
                     /locus_tag="OO349_002977"
     CDS             complement(5797..6318)
                     /gene="sodC"
                     /locus_tag="OO349_002977"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HCR5867974.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGTGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(6399..7295)
                     /locus_tag="OO349_002978"
     CDS             complement(6399..7295)
                     /locus_tag="OO349_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HCR5867975.1"
                     /translation="MVQRITIAPEGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RNHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(7344..7583)
                     /locus_tag="OO349_002979"
     CDS             complement(7344..7583)
                     /locus_tag="OO349_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HCR5867976.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            7686..8285
                     /gene="nemR"
                     /locus_tag="OO349_002980"
     CDS             7686..8285
                     /gene="nemR"
                     /locus_tag="OO349_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HCR5867977.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLAELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            8322..9419
                     /gene="nemA"
                     /locus_tag="OO349_002981"
     CDS             8322..9419
                     /gene="nemA"
                     /locus_tag="OO349_002981"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HCR5867978.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMALQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAAFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQLKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            9500..9907
                     /gene="gloA"
                     /locus_tag="OO349_002982"
     CDS             9500..9907
                     /gene="gloA"
                     /locus_tag="OO349_002982"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HCR5867979.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            10010..10657
                     /gene="rnt"
                     /locus_tag="OO349_002983"
     CDS             10010..10657
                     /gene="rnt"
                     /locus_tag="OO349_002983"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HCR5867980.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            10750..15208
                     /locus_tag="OO349_002984"
                     /pseudo
     CDS             10750..15208
                     /locus_tag="OO349_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
     gene            complement(15252..>15481)
                     /locus_tag="OO349_002985"
     CDS             complement(15252..>15481)
                     /locus_tag="OO349_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5867981.1"
                     /translation="AYTSQRCACCGHTAKENRLSQSKFRCQVCGYTANADVNGARNIL
                     AAGHAVLACGEMVQSGRSLKQEPTEMIQATA"
BASE COUNT         3546 a         4097 c         4071 g         3767 t
ORIGIN      
        1 ccagttgatt aaaggcgcta caaccggaga gaaggaccgg caaaatgata attaacagtt
       61 ttttcatagt catatcccga agactttcct ggtccggagg gcaaataccc tccttaatct
      121 tccaagtgta acggcagaca cgataagaaa aaattcagtt aattctgata tcacgagttg
      181 gcggggaaaa tagcccccag taccgtctcc tggcttgcgc cagtgacgga aggcagattt
      241 cctggtaatc ccgccagcgt ccgccaggca agccaggcga aagccaatgc ttccatgtca
      301 tcgccactaa tgccaacggc atcggtggtg gtgacttctg tgcctagcag taataccgcc
      361 agacgcgcca tgagtagcgg attacgacta cctccaccac ataccatcaa tcgttcgcag
      421 ccaccgctca acaaaacttg ttcagaaatg gtcacggcgg tgagttctgc cagtgtggcc
      481 tgaacatcac ggggatcaac acccggaaaa tggcgtaaat ggcgctccag ccagccgtag
      541 ttaaagtatt cgcgtccggt gcttttcggt gcaggttgcg agaaatacgg gtcgctgagc
      601 atattttgca gcagtgggag aataacttta cccgcccgtg cccactcggc atctttatcg
      661 taaggtttac ctgcctgacg ccagatccag gcatccatca gcatgttacc aggaccggta
      721 tcgtagccgc caaccggctg cccaggaatg agcagtgaca gattggcgat gccgccaata
      781 ttgagcacca ttcgtcgctc ggttgggtga gccagcaagg catgatggaa cgcaggtacc
      841 agcggtgcgc cttgtcctcc caaggcaata tcgcgacggc gaaaatcacc gacaaccgta
      901 attccggtgc gtgccacaat ttgattgtta tcgccaatct gcaaagtgtg tggtgccacg
      961 cccgtcggtt catgccagac ggtttgaccg tgacagccga tcgcaactat atctctcgcc
     1021 tgcaggtttt gttccttaag caaggcattg accgcatcag caaaaagtcg cccgagttga
     1081 gtatcaagct gtccaaactg cgaaagtgta agctgctggc cctggcaaat atccagtaca
     1141 gcctgtttca gagacaccgg gatcggccag ctcaagcttg ccagctgtgc gacccggtgt
     1201 tcatcaattg tcgccaacac aacatcaaca ccatcaaggc tggtgcctga cataacgcca
     1261 ataaagcggc ccgatttcat agttcatcct ttttcaatct gacgtttgcg caccactcaa
     1321 acataaactt ttcgtgaata ccatgcggaa tgaccgattt ttaccgttgg tagtaaaaca
     1381 ttatcttcaa atcaataatc atcatgaatg ttttgtttat aattggttga tcctactttc
     1441 attatgattt gctcatattt ggtagaacat gtaaccatgg attcacatat gccatatact
     1501 ttgaccatga gggatgcttg cgtggcgttt catggtgaac aggagatttt tcaatgatta
     1561 aacgcgtatt ggttgtttca atggtaggtc tgtctcttgt cggttgtgtt aataacgaca
     1621 ccctgtcagg ggatgtttat accgcttctg aagcgaaaca agtacagaat gtcagctatg
     1681 gcaccatcgt taacgtacgt ccggtacaga ttcagggcgg tgatgattcc aacgttatcg
     1741 gtgcaattgg cggtgctgtt cttggtggtt tcctggggaa tactgttggt ggcggaaccg
     1801 ggcgttctct ggctactgca gcaggcgctg ttgcaggtgg cgtagctggt cagggcgtac
     1861 agagtgcaat gaacaaaacg cagggtgtcg agctggaaat tcgtaaagac gatggtaata
     1921 ccatcatggt ggtacagaaa caaggcaaca ctcgtttctc tccgggccaa cgtgtcgtac
     1981 tggccagcaa tggcagtcag gtgaccgttt ctccgcgcta aataagtttg cgtgtggtca
     2041 ggttactgac cacacgcccc cttcatttca ccctttggcc tgtaactcaa tgatattatg
     2101 ctcaagtttt gcgatgagcg taatcagttg ctccagttcc tctgcggaga tgccatgtaa
     2161 tatttccgcg cgggttttgt taataacagc ttccatttcg ctgatcagtg gctctgcctt
     2221 ttccgtcagt ttaatacgtt tagcccgacg atcgctggca caagtttgac gcgaaattaa
     2281 ccctttttct tccagttggt ccagagtacg gaccagtgat ggctgctcga tgccaatcgc
     2341 ttttgccagt tgaatttgcg actggtctgg aggtaactga tggatattgt gtaacgtaac
     2401 ccaatgggtt tgtgttaact ccagcggttt caggcgatgg tctatcagag cacgccatat
     2461 gcgcaccaac cgtgccagat cagaacctag tggcgattcc aatttcatct ccttataatt
     2521 agcttgctaa gatattatgc ggcttttaga atagtgtgca gcaattgtat tgctaaaaca
     2581 aatgtattgc tgcatttggt taccgtcaga catatttttc agaaattgcg cgtaaatttt
     2641 tcgcacttaa agaatattta ttaatctaac gcaatatatt cggtcgtaaa ggaatctact
     2701 ttgtgaagtt tatgctcaat gcaacaggat tgcccttaca agatctggtg ttcggtgcgt
     2761 ccgtctattt tcctccgttt ttcaaagcat tcgcgtttgg attcgtcatc tggcttgtcg
     2821 tacaccgcct gcttcgtggc tggatctacg ccggcgatat ctggcatccc ttgttaatgg
     2881 atttatcgct gtttgcgatt tgcgtttgcc ttgctctggc aatactgatt gcgtggtaac
     2941 tatgtcaatt aaaacaatta agtatttctc aacaatcatt gtagcgatag ttgcggttct
     3001 tgccggatgg tggctgtgga attattacat gcaatctccg tggacacgcg acgggaaaat
     3061 acgcgcagaa caggtttcta tcaccccaca ggtgtcggga cgtattgttg agctgaatat
     3121 aaaagacaac caactggtta acgcggggga acttctgctc acgatcgata aaacgccctt
     3181 tcagatcgcc gagctgaacg cacaggctca gctggcgaaa gcgcaatctg acctcgcaaa
     3241 agccaataac gaagcgaatc gtcgtcgtca tctctcacaa aattttattt ttgccgaaga
     3301 actcgatacc gctaacctca atgttaaagc gatgcaggcc agtgttaatg ccgcacaggc
     3361 gacgctgaaa cagacacaat ggcaactggc gcaaacggaa attcgcgccc cggtgagtgg
     3421 atgggtgact aacctcacta cccgcatcgg cgattacgct gacaccggga aaccgctgtt
     3481 tgcccttgtc gacagccact cgttttacgt cattggttat tttgaagaaa ccaagttgcg
     3541 ccatatccgc gaaggtgcac cagcacaaat taccctctat agtgataaca aaacgttaca
     3601 gggtcacgtt tccagtatcg gtcgggcgat ttatgatcag agcgttgaaa gtgactcaag
     3661 ccttattccg gatgtgaaac ctaacgtccc ctgggttcgt ctcgcccaac tcgttcccgt
     3721 ccgctttgcg ctggataaag ttccaggcga tgtcacgctg gtgtcgggca ccacctgtag
     3781 cattgccgta ggtcaataat gaacgcatcg tcatggtcct tgcgcaattt gccctggttc
     3841 agggccacgc tggcgcaatg gcgttatgcg ttacgcaata ccattgccat gtgtctggcg
     3901 ctgacggttg cctattattt gaatctggat gaaccctatt gggcgatgac ctcggctgca
     3961 gtggttagct ttcccaccgt tggcggtgtc atcagcaaaa gcctcggacg catggctggc
     4021 agtttactgg gagccattgc ggcactgctt cttgccggac atacgctcaa tgagccgtgg
     4081 ttttttctat tgagcatgtc ggcgtggctt ggcttttgta cctaggcctg tgcgcacttc
     4141 acgaataacg tcgcgtatgc atttcaactg gcgggctaca cggctgccat catcgccttt
     4201 ccgatggtta atattactga ggccagccag ctgtgggata tcgctcaggc gcgcgtttgc
     4261 gaggtgatag tcggtatttt gtgcggcggc atgatgatga tgatcctgcc tagcagttcc
     4321 gatgctactg cccttttaac tgcattgaaa aacatgcacg cccgattact ggaacatgcc
     4381 agtttactct ggcagcctga aacaaacgat gccattcgtg cagcacatga gggggtgatt
     4441 gggcagatac tgaccatgaa tttgctgcgt atccaggctt tctggagcca ctatcgtttt
     4501 cgccagcaaa acgcgcgcct taatgcgcta ctccaccagc aattacgtat gaccagtgtc
     4561 atctccagcc tgcgacgtat gttgctcaac tggccctcac cgccaggtgc cacgcgagaa
     4621 attctcgaac aattgctgac ggcgctcgcc agttcgcaaa cagatgctta caccgtcgca
     4681 cgtattatcg ccccgctacg cccgaccaac gtcgccgact accggcacgt cgccttctgg
     4741 cagcgactac gttatttttg ccgcctttat ctgcaaagta gtcaggaatt acatcgtctg
     4801 caaagcggtg tagatgatca tgccagactc gcacggacat ccgctctggc tcgtcatacc
     4861 gacaacgccg aagctatgtg gagcgggctg cgtacatttt gtacgttgat gatgattggc
     4921 gcatggagta ttgcatcgca atgggatgcc ggtgccaatg cattaacgct ggcagcaatt
     4981 agctgcgtac tctactccgc cgtcgcagca ccgtttaagt cgttgtcact gctgatgcgc
     5041 acgctggtgt tacttttggt caaatttggc ctgatggtcc agattagcga tctgtggcaa
     5101 tttttactgt ttctctttcc actgctggcg acaatgcagc ttcttaaatt gcagatgccc
     5161 aaatttgccg cactgtgggg gcaactgatt gtttttatgg gttcgtttat cgctgtcact
     5221 aatcccccgg tgtatgattt tgctgatttt cttaactata atctggcaaa aatcgttggc
     5281 gttgcgctgg cttggctagc gttcgccatt ctgcgtccag gatcggatgc tcgtaaaagc
     5341 cgccgccata ttcgcgcgct gcgccgggat tttgtcgatc agctaagccg ccatccaaca
     5401 ctgagtgaaa gcgaatttga atcgctcact tatcatcacg tcagtcagtt gagtaacagc
     5461 caggatgcgc tggctcgccg ttggttatta cgctggggtg tagtgctgct gaactgttct
     5521 catgttgtct ggcaattgcg cgactgggaa tcgcgttccg atccgttatc gcgagtacgg
     5581 gataactgta tttcactgtt gcggggagtg atgagtgagc gtggcgttca gcaaaaatca
     5641 ctggcggcca cacttgaaga attacagcgg atttgcgaca gccttgcccg tcatcatcaa
     5701 cctgccgccc gtgagctggc ggcaattgtc tggcggctgt actgctcgct ttcgcaactt
     5761 gagcaagcac caccgcaagg tacgctggcc tcgtaattac ttaattacac cacaggcata
     5821 gcgttcaccg ccaccgccca gcggtttagg ttgatcggac atattatcgc cgccaacgtg
     5881 gaccatcagc gccttgtctt tgatttcatc cagtgatttc agacgaggcg cgatgacggc
     5941 atcggtagct ttgccgtcat tattgacgac cagtgcaggc agatcgccta aatgcccggt
     6001 accttctggc ccttcatgtt taccggtatt ttgtggatca agatgcccgc ctgcggattc
     6061 cgcggcgctg gctttgccat ctttggtggc tggctggcag cttcctttgg catgaatatg
     6121 gaagccatgt tcaccggggg gtaatgcttt cagatcgggt gaaaactcca gacctttatc
     6181 ggtttcagta atggtgacgc taccaattga ctgcccaacc ccttgcgacg tgacgaggtt
     6241 catctcgact ttttcactgg cagcttgtgc gccggtcgca acaaccagcg ccagaatggc
     6301 cagactaaaa cgtttcatag gacctccgtt cattgtgcat tcctgaataa gtgtaaacca
     6361 gtgacacatt tttgaacggc ggctattcct aaaagtgctt acggtacgtc gtaccccagt
     6421 gcagctttac ggatacgaaa ccattgttga cgggtcattt tcagtgtttc tgcttcgaca
     6481 gctgcccgta cgcgctcaat tttacctgag ccgataattg gcagcggctg cgacggtaaa
     6541 cgtaataccc aggcgtaaac cacctgttca atcgagcccg cgtttaactc ctccgccacc
     6601 acagccagtt catcacgcag cggctggaaa taatcatcat taaacagacg accaccacca
     6661 aggcaggacc acgccatcgg acgaacacgc agttgttgta gttggtcgag cgtgccatcc
     6721 agcagtaacg gctgatgcac cggggatatt tccacctgat tagtggcaag ggtaaacggc
     6781 agacgtgatt gcaacagggc aaattgcgca ggcgtaaagt tcgatacgcc aaaatgacgc
     6841 actttgccgc tctgatgcag atgtttgaac gcgtccgcca cttcatcggc atccattaac
     6901 gggtctggtc ggtggattaa cagcagatcc agatgatcgg tcgcgagatt aattagcgac
     6961 tgttcggcgc tcttaatgat gtgattgcgg tcagtgatgt aatgaccaat gacgttttct
     7021 tcacgcgcgg tcgtcgcgat accgcattta ctgacgattt ccatccgttc acgcaggtga
     7081 ggtgccagtt tcagtgcctc gccaaacgcc gcttcgcact gatagccacc ataaatatca
     7141 gcatggtcca cggtggtcac gccgagatcc agatgctctt caataaaact gaccagctgg
     7201 cgggcggaca tattccagtc catcaatcgc cagtagccca tcacaaaacg ggaaaactcc
     7261 gggccttccg gcgcaatagt aatacgctga accataatcg cttcctctta tcagatataa
     7321 gaggagtata cgcaagatta ggttcaaaag agtgatggtt gctccggttc gtctgatgac
     7381 gctggcttat ttgcgcgtaa tttgcgcatt aatcgctgcc gacaaaggcg cagcacctct
     7441 tgtttttcgc catcgctcat tttattccag ttaaaacgct catcccgact acgaaaacag
     7501 ccgcgacaaa acccgcgttc gtcagactgg caaattcccc ggcacgggct ctggacggga
     7561 aagaactcta attgctccgc cacctcgccc tcctcagata agattattac cattattgaa
     7621 gctgttaatg tccaaagtag caactttgct tgcactagac cgactggtct actacactcc
     7681 aacgcatgaa caaacacacc gaacatgata ctcgcgaaca tctcttggcg acgggcgagc
     7741 aactttgcct gcaacgtgga ttcaccggga tggggctaag cgaattacta aaaaccgctg
     7801 aagtgccgaa agggtccttc tatcactact ttcgctctaa agaagcgttt ggcgttgcca
     7861 tgcttgagcg tcattacgcc gcatatcacc agcgactggc tgagttgctg caatccggcg
     7921 aaggtaacta ccgcgaccgc atactggctt attaccagca aacactgaac cagttttgcc
     7981 aacatggaac catcagtggt tgcctgacag taaaactctc tgccgaagtg tgcgatctgt
     8041 cagaagatat gcgcagcgcg atggataaag gtgctcgcgg cgtgatcgcc ctgctctcgc
     8101 aggctctgga aaatggccgt gagaaccatt gtttaacctt ttgtggcgaa ccgctacaac
     8161 aggcacaagt gctttacgca ctatggcttg gcgcgaatct gcaggccaaa atttcgcgca
     8221 gtttcgagcc actggaaaac gcgctggccc atgtaaaaaa cattattgcg acgcctgccg
     8281 tttagcaggc attttttatc accagacgac cgggagcctt tatgtcatct gaaaaactgt
     8341 attccccact gaaagtgggc gcgatcacgg cggcaaaccg tatttttatg gcaccgctga
     8401 cgcgtctgcg cagtattgaa ccgggtgaca ttcctacccc gttgatggcg gaatactatc
     8461 gccaacgtgc cagtgccggt ttgattatta gtgaagccac acaaatttct gcccaggcaa
     8521 aaggatatgc aggtgcgcct ggcatccata gtccggagca aattgccgca tggaaaaaaa
     8581 tcaccgctgg cgttcatgct gaaaatggtc atatggcctt gcagctgtgg cacaccggac
     8641 gcatttctca cgccagcctg caacctggcg gtcaggcacc ggtagcgcct tcagcactta
     8701 gcgcgggaac acgtacttct ctgcgcgatg aaaatggtca ggcgatccgc gttgaaacat
     8761 ccatgccgcg tgcgcttgaa ctggaagaga ttccgggtat cgtcaatgat ttccgtcagg
     8821 ccattgctaa cgcgcgtgaa gccgcttttg atctggtaga gctccactct gctcacggtt
     8881 atttgctgca tcagttcctt tccccttctt caaaccatcg taccgatcag tacggcggca
     8941 gcgtggaaaa tcgcgcacgt ttggtactgg aagtggtcga tgccgggatt gaagaatggg
     9001 gtgccgatcg cattggcatt cgcgtttcgc caatcggtac tttccagaac acagataacg
     9061 gcccgaatga agaagccgat gcactgtatc tgattgaaca actgggtaaa cgcggcattg
     9121 cttatctgca tatgtcagaa ccagattggg cggggggtga accgtatact gatgcgttcc
     9181 gcgaaaaagt acgcgcccgt ttccacggtc cgattatcgg cgcaggtgca tacacagtag
     9241 aaaaagctga aacgctgatc ggcaaagggt taattgatgc ggtggcattt ggtcgtgact
     9301 ggattgcgaa cccggatctg gtcgcccgct tgcagctcaa agctgagctt aacccacagc
     9361 gtgccgaaag tttctacggt ggcggcgcgg aaggctatac cgattacccg acgttgtaat
     9421 ccaacattgc gagcggcgta aagccgccgc tatactaaaa caacattttg aatctgttag
     9481 ccattttgag gataaaaaga tgcgtcttct tcataccatg ctgcgcgttg gcgatttgca
     9541 acgctccatc gatttttata ccaaagtgct gggcatgaaa ctgctgcgta ccagcgaaaa
     9601 cccggaatac aaatactcac tggcgtttgt tggctacggc ccggaaaccg aagaagcggt
     9661 gattgaactg acctacaact ggggcgtgga taaatacgaa ctcggcactg cttatggtca
     9721 catcgcgctt agcgtagata acgccgctga agcgtgcgaa aaaatccgtc aaaacggggg
     9781 taacgtgacc cgtgaagcgg gtccggtaaa aggcggtact acggttatcg cgtttgtgga
     9841 agatccggac ggttacaaaa ttgagttaat cgaagagaaa gacgccggtc gcggtctggg
     9901 caactaatct cctgccgggc gtgaactcat cgcgcccgca tctttactgc atcgacaagt
     9961 aatatttgtc ataatgcgcg ctgcaattta tccgtattaa gagaatcaga tgtccgataa
    10021 cgctcaactt accggtctgt gcgaccgttt tcgtggtttt tatcctgttg tgatcgatgt
    10081 tgaaacagcc ggatttaacg ccaaaaccga tgcgctgctt gagattgccg ccatcaccct
    10141 gaaaatggat gaacaaggct ggctgatgcc ggacaccaca ttacatttcc acgtcgaacc
    10201 atttgtcggc gcaaatttgc aaccagaagc cctcgccttc aacggcattg acccgaacga
    10261 tcccgatcgc ggcgcggtca gcgaatacga ggcgctgcac gaaattttta aagttgtacg
    10321 taaaggtatt aaagcgagcg gctgtaaccg cgccattatg gtggcgcaca acgccaattt
    10381 tgatcacagc tttatgatgg ccgccgcaga acgcgcctcg ctgaaacgta acccgttcca
    10441 ccctttcgcc acttttgaca ctgctgcact ggccgggctg gcactcggac aaaccgtatt
    10501 gtcaaaggct tgccagaccg ctggcatgga cttcgacagc acccaggcgc actccgcgct
    10561 gtacgacacc gaacgcactg ctgtgctgtt ttgtgaaatc gtcaaccgct ggaaacgtct
    10621 gggaggctgg ccgctacctg ccgccgaaga ggtgtaatcg agtcgatgcc tgatgacatg
    10681 caatgattca ggcatctata gtgaggctat tccacgcatc aagcatgata ttcacgggga
    10741 atagcgttaa tggcagataa tccagaccct tcatcgctcc tgccggacgt gttttcaccg
    10801 gcgacccgcg actggtttct tcgcgccttt aaacagccga ccgctgtcca gtcgcaaacc
    10861 tggcatgtgg cggcgcgagg cgaacatgcg ctggtgattg caccgactgg ctccgggaaa
    10921 acgctggcag catttctcta cgccctcgat cggctcttcc gcgaaggcgg cgaagatacc
    10981 cgcgaggcgc ataagcgtaa aacctcacgc atcctctata tttcaccgat aaaagccctg
    11041 ggcaccgacg ttcagcgcaa cttgcagatc ccgttgaagg gtattgccga tgaacggcgg
    11101 cggcgcggcg aaacggtaat tattgatgaa gtccacgcgg tagcgggcag taaacgtggt
    11161 gcgcatctgg cgttaagtct ggagcggctc gatgcgctgc tccacacctc agcacagcga
    11221 attggccttt ctgccactgt gcgctcagcc agcgatgtgg cagcatttct tggtggcgat
    11281 cgcccggtta cggtagtcaa cccgcccgca atgcgccatc cgcagatacg aattgtcgta
    11341 ccggtcgcca atatggatga tgtctcatcg gtcgccagcg gcaccggcga agacagccat
    11401 gccggccggg aaggctccat ctggccatat attgaaacgg gtatccttga tgaagtgttg
    11461 cgccatcgct cgaccattgt ctttactaat tcacgcgggc tggcggaaaa actgacagca
    11521 cgattaaatg agctttacgc cgcacgctta cagcgttccc cgtctatcgc cgttgatgcg
    11581 gcccatttcg agtcgacctc cggcgcaacc tctaaccgtg tacaaagtag cgacgttttt
    11641 attgcccgct cacaccacgg ctccgtctct aaagaacaac gagcaatcac cgaacaggcg
    11701 ctgaaatcgg gtgaattacg ctgcgtggtc gcaacctcca gtcttgaact ggggattgat
    11761 atgggcgcgg tggatctggt gattcaggtg gcaacgccgc tttctgttgc cagtgggtta
    11821 caacgcattg gtcgcgccgg acatcaggtt ggcggtgtat ctaaagggct gtttttcccc
    11881 cgtacccggc gtgatttagt cgattccgca gtcattgtag agtgtatgtt cgcaggcagg
    11941 ctggaaaacc tgacaccacc gcataatcct ctcgacgtcc ttgcgcagca aaccgttgcc
    12001 gccgcggcga tggatgcatt acaggtagac gaatggtact cccgcgtacg ccgtaccgca
    12061 ccgtggaaag atctgccaag acgtgttttt gacgccacgc tggatatgct ttccgggcgc
    12121 tatccctctg gcgatttttc tgcttttcgc cccaaactgg tctggaacag ggagaccggg
    12181 atattgaccg cccgacctgg cgctcaattg ttggcggtta ccagcggcgg caccattccg
    12241 gatcgtggca tgtatagcgt gttattaccc gaaggtgaag aaaaggccgg ttcgcggcgg
    12301 gtgggtgaac tggatgagga gatggtatat gagtcgcggg tgaacgacat tatcactctc
    12361 ggcgctacct catggcggat ccagcaaatc acccgcgatc aggtgattgt gactcctgct
    12421 ccgggtcgtt ctgcccagct ccccttctgg cgtggtgaag gtaacggacg tccggctgaa
    12481 ttaggcgaga tgatcggcga ttttcttcat ttactggcgg atggcgcgtt cttttccggg
    12541 actattcccc cgtggctggc agaagaaaat acgaacgcca atattcaggg gttgattgac
    12601 gagcaacgca acgcgacggg catcgttccg gggagtcgcc atctggtcct cgaacggtgc
    12661 cgtgatgaaa ttggcgactg gcgtattatt ttgcactctc cctatggaag acgggtgcat
    12721 gaaccctggg cgctggcgat tgccgggcga atacatgccc tatggggcgc tgacgcgtct
    12781 gtggtcgcca gtgatgacgg cattgttgcg cgcattcctg acactgatgg taaattgccc
    12841 gacgccgcga tttttttgtt tgaaccagaa aagttgctgc aaattgtccg cgaggcggta
    12901 ggcagctcgg cacttttcgc cgcccgtttt cgcgaatgcg ccgcgcgggc attattaatg
    12961 ccgggccgca cgccgggcca tcgcaccccg ctttggcaac aacggctgcg cgccagtcag
    13021 ttgctggaaa tcgctcaggg atatccggat tttccggtca ttctcgaaac cctacgcgaa
    13081 tgtctgcaag atgtttatga tcttcccgca ctggaacgtt tgatgcgtcg cctgaacggt
    13141 ggcgaaattc aaatatccga tgtaacgacc actacgccct cgcctttcgc cacaagttta
    13201 ttgttcggct atgtcgcgga atttatgtac cagagcgacg ccccgctggc agagcgccgg
    13261 gcatccgtac tgtcgctgga cagcgagtta ctgcgcaatc tactcggaca ggtcgatccg
    13321 ggggaattac tcgacccgca ggtcattcgc caggtggaag aagagttgca acgactggct
    13381 cctggtagaa gagcgaaagg tgaagaagga ttgttcgacc tgctgcgcga actggggcca
    13441 atgaccgttg aagacctggc gcaacggcat acaggcagca gtgaagaggt tgcgtcgtat
    13501 ctggaaaatc ttcttgcagt aaaacgaatc ttcccagcga tgattagcgg acaggagcgt
    13561 cttgcctgta tggatgatgc cgccaggctg cgtgatgccc tcggcgtacg gctaccagag
    13621 tcattgccag agatttattt acatagagtc agttacccgc ttcgcgacct ctttctgcgc
    13681 tatctccggg ctcatgctct ggtcacggct gaacaactgg ctcatgagtt tagtctcggt
    13741 attgccattg tcgaagagca gcttcagcaa ctgcgtgaac agggtctggt gatgaatctg
    13801 caacaaaaca tctgggtgag cgatgaagta tttcgtcgtc tgcgtttgcg ctcgctgcaa
    13861 gccgccagag aagcgacgcg tcccgttgca gccacgacct atgcgcgatt gctgctggaa
    13921 cgtcagggcg tattacccgc caccgatggt agcccggcgc tctttgcctc aacatctcca
    13981 ggcgtttatg agggcgtaga tggcgtgatg cgggtgatcg aacagcttgc cggagtcggt
    14041 ttacccgcct cactctggga aagccagatc ctgcctgccc gcgtacgcga ctattcgcca
    14101 gaaatgctcg atgaattact ggcaaccgga gcggttatct ggtcgggaca aaaaaaagct
    14161 gggtgaagat gacggcctgg tggcactgca tctacagaaa tatgccgcag aatcgttcac
    14221 tcccgccgaa gcggatcagg cgaatcgttc ggcgctgcag caagcgataa tcgctgttct
    14281 ggctgacgga ggagcctggt ttgcacaaca aatcagccaa cggatacgcg ataaaatcgt
    14341 cgaatcggtt gattcctcgg cactgcaaga ggcgttatgg gcgctggtct ggcaaggcgt
    14401 cattaccagc gatatttggg caccgttacg cgccctcacc cgcagcagtt ccaacgcacg
    14461 cacctcaact cgccgcagtc accgggctcg tcgtgggcgt cctgtctatg cacaacccgt
    14521 ctcgccgcgg gtatcttaca acacaccaaa tctggctgga cgctggtcgt tattgcaggt
    14581 ggagccacta aacgataccg aaaggatgct ggcgctggcg gaaaatatgc tcgaccgcta
    14641 cggcatcatc agtcgtcagg cggtgatagc cgaaaatatc cctggcgggt ttccatcgat
    14701 gcaaacactt tgtcgaagta tggaagactc cgggcgaatt atgcgaggtc gttttgtaga
    14761 aggtctgggt ggcgcgcaat tcgctgaacg tctgactatt gaccgattgc gcgatctggc
    14821 gacacaagcc acgcaaacac gccactatac accagtggcg ctctccgcca acgatccggc
    14881 taatgtgtgg ggaaatcttc tgccctggcc tgcacatccg gcaacgctgg taccaacgcg
    14941 tcgggcgggt gcgctggtgg tcgtttctgg cggcaaattg ttactctatc tggcgcaagg
    15001 cggcaaaaaa atgctggtct ggcaggaaaa ggaggaatta ctcgccccag agattttcca
    15061 ggcgctgacc accgcactgc gtcgcgaacc aaggctgcgc tttacgctaa cagaagtgaa
    15121 tgatcttccg gtccggcaaa cgccgatgtt tacgctgctg cgtgaggcgg gattttcaag
    15181 ttcgccacaa gggctggatt ggggatagtt gacatcctcc acgccctgaa agacgtggtt
    15241 ccctgctacg ttcaagctgt cgcctgaatc atttcggtgg gttcctgctt caacgagcgg
    15301 cctgactgca ccatctctcc acaggcaaga acggcgtgcc ccgccgctaa aatgttacga
    15361 gcgccattta catcggcgtt cgctgtatat ccacatacct ggcatctgaa tttactttgt
    15421 gacaggcggt tttctttcgc tgtatgacca cagcacgcgc aacgctggct tgtgtacgct
    15481 g
//
LOCUS       DAJTQY010000086        15410 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.086, whole genome shotgun sequence.
ACCESSION   DAJTQY010000086 DAJTQY010000000
VERSION     DAJTQY010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15410
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.086"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(<1..1175)
                     /locus_tag="OO349_002986"
     CDS             complement(<1..1175)
                     /locus_tag="OO349_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCR5867982.1"
                     /translation="MALSDVKVRSAKPEAKAYKLTDGEGMVLLVHPNGSKYWRLRYRF
                     GGKEKMLALGKYPEVSLADARARRDEARKLLANGVDPSENKKAVKVEQEQEAITFEVV
                     ARDWHASNQKWSASHSARVLKSLEDNLFTAIGKRNIAELKTRDLLVPIKAVESSGRLE
                     VAARLQQRTTAIMRFAVQSGLIDYNPAQEIAGAVATAKRQHRAALELNRIPELLHRID
                     HYSGRPLTRLAVELTLLVFIRSSELRFARWSEIDFETAMWTIPGEREQLEGVKHSQRG
                     SKMRTPHLVPLSRQALSILEKIKSMSGNRELIFVGDHDPRKPMSENTVNKALRVMGYD
                     TKVEVCGHGFRTMACSSLIESGLWSRDAVERQMSHQERSSVRAAYIHKAEHLGERRL"
     gene            complement(1374..1458)
                     /locus_tag="OO349_002987"
     tRNA            complement(1374..1458)
                     /locus_tag="OO349_002987"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1422..1424),aa:Leu,seq:caa)
     gene            1654..2673
                     /gene="ahr"
                     /locus_tag="OO349_002988"
     CDS             1654..2673
                     /gene="ahr"
                     /locus_tag="OO349_002988"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HCR5867983.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            2802..4304
                     /locus_tag="OO349_002989"
     CDS             2802..4304
                     /locus_tag="OO349_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HCR5867984.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLTRLKNIGVNDWQPHANPVVVWDIFG
                     EKGHTVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVFVQLGNRVQHALRAFTPKDQKAV
                     KTAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(4423..5505)
                     /gene="lptG"
                     /locus_tag="OO349_002990"
     CDS             complement(4423..5505)
                     /gene="lptG"
                     /locus_tag="OO349_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HCR5867985.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMVIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEVLGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTDLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(5505..6605)
                     /gene="lptF"
                     /locus_tag="OO349_002991"
     CDS             complement(5505..6605)
                     /gene="lptF"
                     /locus_tag="OO349_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HCR5867986.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            6872..8383
                     /gene="pepA"
                     /locus_tag="OO349_002992"
     CDS             6872..8383
                     /gene="pepA"
                     /locus_tag="OO349_002992"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HCR5867987.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            8517..8960
                     /gene="holC"
                     /locus_tag="OO349_002993"
     CDS             8517..8960
                     /gene="holC"
                     /locus_tag="OO349_002993"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HCR5867988.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGSAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            8960..11815
                     /gene="valS"
                     /locus_tag="OO349_002994"
     CDS             8960..11815
                     /gene="valS"
                     /locus_tag="OO349_002994"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HCR5867989.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDICESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGANVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(11869..13065)
                     /locus_tag="OO349_002995"
     CDS             complement(11869..13065)
                     /locus_tag="OO349_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 domain-containing protein"
                     /protein_id="HCR5867990.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICMVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLVGCV
                     LTLLLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSLKGFWWVTFFLPILMAIGMGTV
                     FFISTKMLHANSSSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTAYCIKYAILAFLALLLFLAVAGYIIFDQILNEYDSSGYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGVM
                     PSLPFL"
     gene            13258..13761
                     /locus_tag="OO349_002996"
     CDS             13258..13761
                     /locus_tag="OO349_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5867991.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIASVIRQVYAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYNGEVVGGGGIAPLTGSEADICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(13807..14223)
                     /gene="rraB"
                     /locus_tag="OO349_002997"
     CDS             complement(13807..14223)
                     /gene="rraB"
                     /locus_tag="OO349_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HCR5867992.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            14385..15398
                     /gene="argF"
                     /locus_tag="OO349_002998"
     CDS             14385..15398
                     /gene="argF"
                     /locus_tag="OO349_002998"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCR5867993.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     LNN"
BASE COUNT         3976 a         3932 c         3966 g         3536 t
ORIGIN      
        1 atcaacctac gctccccaag atgttccgcc ttatggatgt aagctgctcg cacagagcta
       61 cgctcctggt gactcatctg ccgctctacc gcatccctcg accataatcc cgactcaatc
      121 aacgaactac aagccatcgt cctgaagcca tgcccacaaa cctcgacctt cgtgtcatac
      181 cccattacgc gcaaagcctt gttcaccgta ttctcactca tcggcttacg tggatcgtga
      241 tcgcccacaa aaatcagctc tcgatttccg ctcatgcttt tgatcttttc caaaatactt
      301 agggcctgcc gcgacaaagg tacaaggtgt ggtgtccgca tcttcgagcc acgctgagaa
      361 tgcttgactc cttccagttg ctcacgctca cctgggattg tccacatagc cgtttcaaaa
      421 tctatttctg accagcgggc aaaacgcagc tcgctcgaac ggatgaaaac caataaagtg
      481 agttcaaccg ccagtcgagt taacggtctg ccagaatagt gatctatgcg atgaagtaat
      541 tcaggtatac ggttcagttc aagagctgca cggtgctgtc ttttcgccgt agcaaccgca
      601 cctgcaatct cttgcgcggg gttgtagtcg attaagccgc tctgaacggc aaagcgcata
      661 atcgcggtag tacgctgctg taaacgggcg gcaacttcga gccgcccgga tgactcgact
      721 gctttaatgg gtacaagaag atcccgcgtc ttcagttccg caatgttccg cttaccgata
      781 gcagtaaaga gattatcctc cagacttttc aaaacacgag cgctatgcga tgccgaccac
      841 ttctgattgc tggcgtgcca gtctctggcg actacttcaa acgttatcgc ctcttgttcc
      901 tgttctacct taacagcttt cttgttttca ctgggatcga caccattagc taaaagctta
      961 cgagcctcat cccggcgtgc cctggcatcc gccaacgaca cttcggggta tttccccaat
     1021 gccagcatct tctctttgcc gccaaagcga taacgtagcc gccagtattt ggagccgtta
     1081 gggtgaacca gcaaaaccat gccttcaccg tcggtcagct tataggcttt tgcttcaggc
     1141 ttagccgaac gaaccttcac atcactcaga gccatgatga gtatcctttc aagggttctg
     1201 tgtgggtaca aacattatcg aaccgggaaa tacccgcagt tgtacccgca tcagtaagtt
     1261 gatgtagatt gaatcaggtt gacttaggtt gagtgaaaaa gcgaggaaag ccttgcggat
     1321 actggatttt aggcacaaaa aaagacgtcc gttgacgtct attgatgttc cgatggtgcc
     1381 gaaggccgga ctcgaaccgg cacgtatttc tacggttgat tttgaatcaa ctgcgtctac
     1441 cgatttcgcc acttcggcac tgaagaggta tgcggaaaac gttgtggatt atacctgtcg
     1501 cacgccacca tgcaagcgcc agcttgcacc cacctcgcta agtgctgaaa ttttcagcat
     1561 tacactcagc gatatgtaac ctttgtcaca ctccaggcac cccgccctgc catgctctac
     1621 acttcccaaa caacaccaga gaaggcccca aaaatgtcga tgataaaaag ctatgccgca
     1681 aaagaagcgg gcggcgaact ggaagtttat gagtacgatc ccggtgagct gaagccacaa
     1741 gatgttgaag tgcaggtgga ttactgcggg atttgtcatt ccgatctgtc gatgatcgat
     1801 aacgaatggg gattttcaca atatccgctg gttgccgggc atgaggtgat tgggcgcgta
     1861 gtggcactcg ggagcgccgc gcaggataaa ggtttgcagg tcggtcagcg tgtcgggatt
     1921 ggctggacgg cgcgtagctg tggtcactgc gacgcctgta ttagcggtaa tcagatcaac
     1981 tgcgagcaag gtgcggtgcc gacaattatg aatcgcggtg gctttgccga gaagttgcgt
     2041 gcggactggc aatgggtgat tccactgcca gaaaatattg atatcgagtc cgccgggccg
     2101 ctgttgtgcg gcggtatcac ggtctttaaa ccactgttga tgcaccatat cactgctacc
     2161 agccgcgttg gggtaattgg tattggcggg ctggggcata tcgctataaa acttctgcac
     2221 gcaatgggat gcgaggtgac ggcctttagt tctaatccgg cgaaagagca ggaagtactg
     2281 gcgatgggtg ccgataaagt ggtgaatagc cgcgatccgc aggcactgaa agccctgtcg
     2341 gggcagtttg atctcattat caatactgtg aacgtcagcc tcgactggca gccttatttt
     2401 gaggcgctga cctatggcgg taatttccat acggtcggtg cggttctcac gccgctgtct
     2461 gttccggcct ttacgttaat tgcgggcgac cgcagcatct ctggttctgc taccggcacg
     2521 ccttatgagc tgcgaaagct gatgcgcttt gccgcccgca gcaaggttgc gccgacaacc
     2581 gaactgttcc cgatgtcgaa aattaacgac gccattcagc atgtacgcga tggtaaagcg
     2641 cgctaccgag tagtcctgaa agccgacttc tgatcccttt gccgggtgct gtcgcccggc
     2701 acttaaatct ctttggaagg cgtctcgcaa tctgctgaat ttagcggtga agagtgcaca
     2761 gccgttgcct atactccagg caggaaactg gaggaaatct catgagcgaa cccctgttaa
     2821 ttgcccgcac gccggacaca gaactgtttt tactgccggg aatggctaac cgtcacgggc
     2881 tgattactgg cgcaacgggg acgggtaaaa ccgttacgct gcaaaaactg gcagagtcat
     2941 tgtcggaaat cggcgtgccg gtgtttatgg ctgatgtgaa aggcgatctg accggtatcg
     3001 cgcaggcagg aacggcgtcg gaaaaactgc tcacaaggct taaaaatatc ggcgtcaatg
     3061 actggcaacc gcatgccaat ccggtggtgg tgtgggatat ctttggcgag aaaggccaca
     3121 cggtgcgggc gacggtttcg gatctggggc cgctgttgct ggcgcgactg ttgaatctca
     3181 acgatgtgca atctggcgtg ctgaatatta tcttccgcat tgcggacgat cagggattgt
     3241 tgctgctcga ctttaaagat ttgcgggcga ttacccagta catcggcgat aacgccaaat
     3301 ccttccagaa tcagtacgga aatatcagta gcgcatcggt tggtgccatc cagcgcggat
     3361 tactgtcgct ggaacagcaa ggcgcagcac acttctttgg tgagccgatg ctggatatca
     3421 aagactggat gcgcaccgat gccaacggta aaggcgttat caatatcctc agcgccgaga
     3481 aactttatca gatgccgaaa ctgtacgccg ccagcctgct gtggatgctt tcagagttgt
     3541 atgaacaatt gccggaagcg ggcgatctgg agaaaccaaa actggtgttt ttcttcgacg
     3601 aggcacatct gctgtttaac gatgcaccgc aggtactgct ggataagatt gagcaggtga
     3661 taaggcttat tcgctcaaaa ggcgtgggcg tctggttcgt ttcgcaaaac ccgtctgata
     3721 ttccggataa tgtgttcgtc cagctcggta atcgtgttca gcacgctttg cgggctttta
     3781 cgccaaaaga tcagaaagca gtgaagacag cggcgcaaac catgcgggcc aatccggcat
     3841 ttgataccga aaaggcaatc caggaactgg ggaccggcga ggcgttaatc tcgtttctcg
     3901 atgcaaaagg aagtccttct gtggtggaac gggcgatggt gatcgcacct tgttcgcgaa
     3961 tggggccggt gacggaagat gagcgtaatg gcctgattaa tcactccccg gtgtatggca
     4021 aatatgaaga tgaggtggac cgcgagtccg cctatgagat gctgcaaaaa ggctttcagg
     4081 ccagtaccga gcagcaaaat aatccccccg tgaaagggaa agaggtggcg gtggatgacg
     4141 gtattcttgg tggattgaag gatattttgt ttggcactac cggaccacgc ggcgggaaga
     4201 aagatggtgt ggtgcaaaca atggcgaaaa gcgccgctcg ccaggtgacg aatcagattg
     4261 tacgcgggat gttggggagt ttgctggggg ggagaaaaag ataagtcaga cgtgatctgt
     4321 gcccctcacc ctaaccctct ccccaaaggg gcgaggggac cgttcgaact cgtatttgac
     4381 tttgtcaaca ataataaagg gagctttcgc tcccttgtta gattacgatt ttctcattaa
     4441 cagccacagg ctgattaaga agaagctggc acttggcaac agtgcgccga tgatcggcgg
     4501 gatgccgtaa accaacgtca gcgggccgaa gatctggtcc agtacgtaga agacaaaacc
     4561 gaagctgata cctgtgacca cacgcacgcc catcggtacg ctacgcagtg ggccaaagat
     4621 aaacgacagc gccatcagca tcatcaccgc cacggatagc ggctggaaga ttttgctcca
     4681 catgttgagc tgataacgtc cggcatcctg accgctcgac ttcagatact tcacgtagtt
     4741 gtgcaaacca ctgatggaga gtgcatccgg gtccagcgcc accacgccca gtttgtctgg
     4801 cgtgaggtcg gttttccagg tgccgctcac cgtctgcgaa ccggtaatct gtttcggatt
     4861 ggtcagatca gattcatcaa cctgcgacag acgccagact ttatgttccg ggtcaaactt
     4921 cgcggtagcg gcataacgta cggattgcag acgacgattc tcgttaaagg cataaatact
     4981 gatgccacct aacacctcgt cacctttaac ccgctcaatg tagacgaagt tgttgccatc
     5041 tttcgcccat aaaccttgct gggtagagag caacgagccg ccgtacatcg cctgcgcacg
     5101 gtagttacgc gccatctgtt cgccctgcgg cgcgacccat tcgccaatca ccatcgtcag
     5161 caagaccagt ggaatggcgg ttttcatcac cgacagcgct acctgcatac gggtaaaacc
     5221 agaagcctgc atcaccacca gttcgctgcg ctgcgccagc atcccaagac caagcaacgc
     5281 cccaagaaga gccgccatcg ggaagaagat ctgcacatct ttcggcacgc tcagcaaggt
     5341 atacattcct gcgcctaacg cgtcgtaact cccctgcccg gcttttttca gctgatcgac
     5401 aaatttgata atgcccgaca gcgacaccag catgaacagc gtcatcatga tggtggtgaa
     5461 aatagtttta ccgatatagc ggtcaagtac gccaaaaggt tgcatcacac cgctccttta
     5521 cgcgaaaaac tggcgcgcag gcggcggacc ggcacggtgt cccaaaggtt gagaacaatc
     5581 gccaaagcca gataaatcag gttaacggtc cacatccaca gtgtcgggtc cagcttacct
     5641 ttaccgccgt tcgatttcag ggaggtctgg atcaggaaga aaagtagata cagcagcatg
     5701 gctggcagca tcgacagtac gcgtccctga cgcgggttaa ccacgctcaa cggtacaacc
     5761 ataagtgcca tcataaacac ggtgaatacc aacgtgatac gccagttcag ttctgcgcga
     5821 gcacgatcgg tgtcagtgtt ccacaatgtg cgcatgtcca tctggtcggt atcgttcggg
     5881 tcgagcgcca ccgcctggtg accaatgatc gcctgataat cctggaaatc cgtaatgcgg
     5941 aaatcacgta acagtgcagt gccttcgaag cgcgttccct ggttgagagt gacgacctgg
     6001 gagccgtcgc gcagctgggt taaatgtccg gaatcggcca ccaccacaga aggacgtgca
     6061 ttaccttttg gtcgaatttg tgcgaggaac acatctttga aatcgctgcc gtcaacgctt
     6121 tcgatgaaca gcaccgagct gccattagtc gcttgctgga attgcccttg cgccagcgcc
     6181 gccatgccag ggttcgcttt cgcttctgct aacacttcat cctgatgacg cgatgaccac
     6241 ggtcccgccc acatcacgtt aaccgccgcg acgattgccg tgaataccgc aaggatcatt
     6301 gccgctttca ccagaaccgc tttgctcaag ccgcaggcat gcattaccgt aatttcactt
     6361 tcggtataca gtttgcccag cgtcatcagc agcccgagga acaggcttaa tggcaggata
     6421 agctgcgcca tttccggcac gcccaacccg agaagggaga gcaccagatt cgccggaata
     6481 tcgccgtcaa ccgctgcgcc gaggatcctc actaactttt gacagaagaa gatcaaaagc
     6541 aagatgaaga gtatcgccag ctggcttttg agcgtctccc gcaccagata tcttatgatt
     6601 atcactttaa atacgcccgt aaaaactcgt cttttgcagg attttagctt gtttcatggc
     6661 ttaaacgtca tttattctct tgagtcgtcg aaatcgtcgc taagataatt atactcaacg
     6721 gattcacctc tcagattttg ttctgacgtg ccaatgccgt aataacgtta agattaacac
     6781 gaagtcatcg caacagcgga catgagttac gaaagcttgc aattctatct gtagccaccg
     6841 ccgttgtctt taagattcag gagcgtagtg catggagttt agtgtaaaaa gcggtagccc
     6901 ggagaaacag cggagtgcct gcatcgtcgt gggcgtcttc gaaccacgtc gcctttctcc
     6961 gattgcagaa cagctcgata aaatcagcga tgggtacatc agcgccctgc tacgccgggg
     7021 cgaactggaa ggaaaaccgg ggcagacatt gttgctgcac catgttccga atgtactttc
     7081 cgagcgaatt ctccttattg gttgcggcaa agaacgtgag ctggatgagc gtcagtacaa
     7141 gcaggttatt cagaaaacca ttaatacgct gaatgatact ggctcgatgg aagcggtctg
     7201 ctttctgact gaactgcacg ttaaaggccg taacaactac tggaaagtgc gtcaggctgt
     7261 cgagacagca aaagagacgc tctacagttt cgatcagttg aaaacgaata agagcgaacc
     7321 gcgtcgtccg ctgcgtaaaa tggtgttcaa cgtgccgacc cgccgtgaac tgaccagcgg
     7381 tgagcgcgcg atccagcacg gtctggcgat tgccgccggg ataaaagcag cgaaagatct
     7441 cggtaatatg ccgccgaata tctgtaacgc cgcttatctc gcctctcagg cgcgccagct
     7501 ggctgacagc tacagcaaga atgtcatcac ccgcgttatc ggcgaacagc agatgaaaga
     7561 gctgggaatg cactcttatc tggcggtcgg tcagggttca caaaacgaat cgctgatgtc
     7621 ggtgattgag tacaaaggca acgcgtcgga agatgcacgc ccaatcgtgc tggtgggtaa
     7681 aggtttaacc ttcgactccg gcggtatctc catcaagcct tcagaaggca tggatgagat
     7741 gaagtacgat atgtgcggcg cggcggcagt ttacggcgtg atgcgtatgg tcgcggagct
     7801 gcaactgccg attaacgtta ttggcgtgct ggcaggctgt gaaaacatgc ctggcgggcg
     7861 tgcctatcgt ccgggcgatg tgttaaccac catgtccggt caaaccgttg aagtgctgaa
     7921 caccgatgcc gaaggccgcc tggtactgtg cgacgtgtta acttacgttg aacgttttga
     7981 gccggaagca gtgattgacg tggcgacgct gaccggtgcc tgcgtgatcg cgctgggtca
     8041 tcatattact ggtctgatgg cgaaccataa tccgctggcc catgaactga ttgccgcgtc
     8101 tgaacaatcc ggtgaccgcg catggcgctt accgctgggt gacgagtatc aggaacagct
     8161 ggagtccaat tttgccgata tggcgaacat tggcggtcgt cctggtgggg cgattaccgc
     8221 aggttgcttc ctgtcacgct ttacccgtaa gtataactgg gcgcacctgg atattgcagg
     8281 aaccgcctgg cgttctggta aagcaaaagg cgcaaccggt cgtccggtag cgttgctggc
     8341 acagttcctg ttaaaccgcg ctgggtttaa cggcgaagag taagtttgcg ttttaccctc
     8401 accctaaccc tctccctaag ggagagggaa ccgatcgagc gcaatagaaa ttcattgctc
     8461 atctttcagg gtgaggaatg cagcaatatt taaatccacc acaagaagcc ccatatatga
     8521 aaaacgcaac gttctacctt ctggacaatg acaccaccgt cgatggctta agcgccgttg
     8581 agcaactggt gtgtgaaatt gccgcagaac gttggcgcag cggtaagcgc gtactcatcg
     8641 cctgtgaaga tgaaaagcag gcttaccggc tggatgaagc cctgtgggcg cgtccggcag
     8701 aaagctttgt tccgcataat ttagcgggag aaggaccgcg cggcagtgcg ccggtggaga
     8761 tcgcctggcc gcaaaaacgc agcagcagcc cgcgcgatat attgattagt ctgcgaacaa
     8821 gctttgcaga ttttgccacc gctttcacag aagtggtaga cttcgttcct tatgaagatt
     8881 ctctgaaaca actggcgcgc gaacgctata aagcctaccg cgtggctggt ttcaacctga
     8941 atacggcaac ctggaaataa tggaaaagac atacaaccca caagatatcg aacagccgct
     9001 ttacgagcac tgggaaaagc agggctactt taagcctaat ggcgatgaaa gccaggaaag
     9061 tttctgcatc atgatcccgc cgccgaacgt caccggcagt ttgcatatgg gtcacgcctt
     9121 ccagcaaacc atcatggata ccatgatccg ctatcagcgc atgcagggca aaaacaccct
     9181 gtggcaggtc ggtactgacc acgccgggat cgctacccag atggtcgttg agcgcaagat
     9241 tgccgcagaa gaaggtaaaa cccgtcacga ctacggccgc gaagctttca tcgacaaaat
     9301 ctgggaatgg aaagcggaat ctggcggcac cattacccgt cagatgcgcc gtctcggcaa
     9361 ctccgtcgac tgggagcgtg aacgcttcac catggacgaa ggcctgtcca atgcggtgaa
     9421 agaagttttc gttcgtctgt ataaagaaga cctgatttac cgtggcaagc gcctggtaaa
     9481 ctgggacccg aaactgcgta ccgctatttc tgacctggaa gtggaaaacc gcgaatcgaa
     9541 aggttcgatg tggcacatcc gctatccgct ggctgacggc gcgaaaaccg cagacggtaa
     9601 agattatctg gtggttgcga ccactcgccc ggaaaccctg ctgggcgata ccggtgtagc
     9661 tgttaacccg gaagatccgc gttacaaaga tctgattggc aaatatgtca ttctgccgct
     9721 ggttaaccgt cgtattccga tcgttggcga cgaacacgcc gacatggaaa aaggcaccgg
     9781 ctgcgtgaag atcaccccgg cgcacgactt taacgactat gaagtcggta aacgtcacgc
     9841 cctgccgatg atcaacatcc tgacctttga cggtgatatc tgtgaaagcg cccaggtgtt
     9901 cgataccaaa ggtaacgaat ctgacgttta ttccagcgag atcccggcag agttccagaa
     9961 actcgagcgt tttgctgcac gtaaagcagt cgttgctgcg gttgatgcac tcggcctgct
    10021 ggaagaaatt aaaccgcacg acctgaccgt tccttacggc gaccgtggcg gcgtggttat
    10081 cgaaccgatg ctgaccgacc agtggtacgt gcgtgccgat gtactggcga aaccggcagt
    10141 tgaagcggtt gagaacggcg acattcagtt cgtaccgaag cagtacgaaa acatgtactt
    10201 ctcctggatg cgcgatattc aggactggtg tatctctcgt cagctgtggt ggggtcaccg
    10261 tatcccggca tggtatgacg aagcgggtaa tgtttatgtt ggccgcaacg aagaagaagt
    10321 gcgcaaggaa aataacctcg gtgccaacgt tgccctgcgt caggacgaag acgttctcga
    10381 cacctggttc tcttctgcac tgtggacctt ctctaccctt ggctggccgg aaaataccga
    10441 cgccctgcgt cagttccacc caaccagcgt gatggtatct ggtttcgaca tcatcttctt
    10501 ctggattgcc cgcatgatca tgatgaccat gcacttcatc aaagatgaaa atggcaaacc
    10561 gcaggtgccg ttccacactg tttacatgac cggtctgatt cgtgacgacg aaggtcagaa
    10621 gatgtccaaa tccaagggta acgttatcga cccgctggat atggttgacg gtatttcgct
    10681 gccagaactg ctggaaaaac gcaccggcaa tatgatgcag ccgcagctgg cggacaaaat
    10741 ccgtaagcgc accgagaaac agttcccgaa cggcattgag ccgcacggca ccgatgccct
    10801 gcgcttcacc ctggcggcgc tggcgtctac cggtcgtgac atcaactggg atatgaagcg
    10861 tctggaaggc taccgtaact tctgtaacaa gctgtggaac gccagccgct ttgtgctgat
    10921 gaacacagaa ggtcaggatt gcggcttcaa cggcggcgaa atgacgctat cgctggcgga
    10981 ccgctggatt ctggcggagt tcaaccagac tatcaaagcg taccgcgaag cgctggacag
    11041 cttccgcttc gatatcgccg caggcatttt gtatgagttc acctggaacc agttctgcga
    11101 ctggtatctg gagctgacca agccggtaat gaacggtgga acagaagccg aactgcgcgg
    11161 tactcgccat acgctggtga ctgtactgga aggtctgctg cgcctcgcgc atccgatcat
    11221 tccgttcatc accgaaacca tctggcagcg tgtgaaagta ctttgcggta tcactgccga
    11281 caccatcatg ctgcagccgt tcccgcagta tgatgcatct caggttgatg aagccgcact
    11341 ggccgacacc gagtggctga aacaggcgat cgttgctgta cgtaacatcc gtgcagaaat
    11401 gaacatcgcg ccgggcaaac cgctggagct gctgctgcgt ggttgcagcg cggatgcaga
    11461 acgtcgcgta aatgaaaacc gtggcttcct gcaaacgctg gcgcgtctgg aaagcatcac
    11521 cgtgctgcct gccgatgaca aaggtccggt ttccgttacc aagatcatcg acggcgcaga
    11581 gctgctgatc ccgatggctg gcctcatcaa caaagaagat gagctggcgc gcctggcgaa
    11641 agaagtggcg aagatcgaag gtgaaatcag ccgcatcgag aacaagctgg caaatgaagg
    11701 ctttgtcgcc cgcgcaccgg aagcggttat cgcgaaagag cgtgagaagc tggaaggtta
    11761 tgcagaagcg aaagccaagc tgattgaaca gcaggctgtt atcgccgcac tgtaatcaac
    11821 acagtgatga aaacgaaggc cggagcatgc tccggccttt tttatctctt acagaaatgg
    11881 taaagaaggc ataactcccc gagaaatact ggctaagaag cctttatctg gttgttcttc
    11941 tttattgatt aaaggaaggt catccaaatc gcccagcaaa tacgtatttt ttgcctgcca
    12001 gtcaatcatc catattttca gcagtgggta agccagacca cccgtaatcc cggatatcac
    12061 caccaacgca cacatgcgat aaagcatacc gtggtacgtt aaagttgagc gaaaacgaat
    12121 acgcccatca ttcagtgaca ggttgctcat aaaatgattt cgcaacgaca ccgttaaata
    12181 actggtgcta acagcaatcc caaaataata aattaactgc gcgattatca ttttacgttg
    12241 catttccata aattgctgta aattctcaat atcatcattt gcatacccag aactatcata
    12301 ctcatttaat atttgatcga agataatata accagcaaca gcgagaaaaa gtaataaagc
    12361 taaaaatgcg aggatggcat attttataca atacgcagta tctaatttca ccttgaaacg
    12421 atgtataccg aaactggtat tgctccagag aaaactcatt accaggctat ataaagtacc
    12481 attaaaaata ccaatggaaa caataccaac tattgccatc agaaccacag atataataac
    12541 actacttgaa ctattggcat gtagcatctt tgtcgagata aagaaaacag tccccatccc
    12601 aatggccatt aaaatcggca agaaaaaggt tacccaccag aaccctttca aagagcaatt
    12661 aaaactaaat cttacgccgt tgagactggt cattaaggcc tgataacgca gtccttttgc
    12721 tgccataaat ataagcaaaa ccaacagtaa caaagtcaaa acacagccaa caagcggcat
    12781 atctgctgac actgtcatta agattgcgaa atagaaaaaa acaaaaacaa gacaactaac
    12841 aaaaacattc ccaccggtaa ttccataaga aaatcgttgt ccgttaactt ccatattagc
    12901 ataaagataa cgcttacatt tcattaatgc ccatggtaaa tagataccta gcgttataat
    12961 cgttaacaac acattcacca tacaaataag aaaatatctc tcacctgtac catgaaaaac
    13021 aaaagaatgg gacggaacat ccatttcatt aataacttga gccataaaaa tcccctgaat
    13081 taaaaaatcc attgatataa tttatagata cagacagtaa aatgttctgc cacgttacaa
    13141 tactcttatc agtaataggc tggcaaaaac tttattgctt tataagacgc ttgcgcacgt
    13201 aagaagagag tggtattaaa caaatgtata attactttat ttttattggg ttaaactatg
    13261 aataacattg cgccgcaatc acctgtaatg cgtcgcctga cgctgcagga taatcctgct
    13321 atcgccagcg tcatccgtca ggtttacgca gagtatggtc ttaccgccga taaaggctac
    13381 accgttgccg atccgaatct cgacgaactg tatcaagtgt acagccagcc tggccatgct
    13441 tattgggtgg ttgaatacaa tggcgaagtg gtcggcggcg gcgggattgc gccattaaca
    13501 ggtagtgaag cggacatttg cgaactgcag aaaatgtact ttcttcccgc tattcgcggt
    13561 aaagggctag cgaaaaaact ggccttaatg gcgatggagc aggcacgaga gatgggcttc
    13621 aaacgctgct atctggaaac gaccgctttt ttaaaggaag ccattgcgct ttatgagcat
    13681 ttgggctttg agcatatcga ctatgcgctt ggctgcacgg gccatgtcga ttgtgaagtg
    13741 cggatgctac gcgaactcta aaaaaatgcc cgctagtatg tgctatcggg cattttaatc
    13801 aattaattag tggcgaaccc cgtcatcgtc ttcatcgaca aaatcttcat cgtcgccatc
    13861 ttcgccgtta ggatcttcaa agtaagtgcc ccaaccgtcg tactcaacgt caaatttctc
    13921 cgccagcgtc atcagttgtt caacctgggc atcgatcaga tcggcattca acgcgcactc
    13981 gctgaggatg tcgcagcaaa tcacgatatc accatcttca acttccagct cttctggatc
    14041 ggtcacttcg taaccgagtt taaacgcttc aactgccgct ttttccagag tttccagatc
    14101 gtctgcggaa agatggtgtt cgatggtgta cagcgcgtcc ggatcgctgc catcttccag
    14161 taattcttca ataatcaaac gtgtttcttc acgctgttct tccagttgtt ccgggtttgc
    14221 catggctcat tcctcttaat gtcctgccga tactcttatt gtcacacacc actgacattg
    14281 cctccacctt tgtgacaaag atttatgctt tagacttgca aatgaataat aatacacata
    14341 aattgaattt taattcattg aggcgttagc cacaggaggg atctatgtcc gggttttatc
    14401 ataagcattt cctgaaatta ctcgatttca cgccagctga actcaacagc ctgctgcagt
    14461 tagccgcgaa gctgaaagct gataagaaaa gcggtaaaga agaagccaaa ctcactggta
    14521 aaaacatcgc gctcatcttc gaaaaagact cgactcgtac ccgatgctct ttcgaagttg
    14581 ccgcatatga ccagggggct cgcgttactt atcttggccc aagcggtagt cagattggtc
    14641 ataaagagtc gattaaagac actgcccgcg tgcttggtcg catgtatgac ggtattcagt
    14701 atcgcggcta tggtcaggag attgtcgaaa cactggcgga atacgctggc gtgccggtat
    14761 ggaacggcct gaccaatgag tttcacccca cgcagttgct ggcggatctc ctcaccatgc
    14821 aggagcattt acccggtaaa gcattcaacg aaatgacgct ggtctatgca ggtgacgcac
    14881 gtaacaacat gggcaattcg atgctcgaag ctgcggcgct taccggtctg gatttgcgtc
    14941 tggtcgcgcc acaggcatgc tggccagaag ctgcgctggt tacggaatgc cgcgccctgg
    15001 cacaacaaaa tggcgggaat attacgctga cggaagatgt agctaaagga gttgaaggtg
    15061 ctgactttat ctataccgat gtatgggtgt cgatgggtga agcaaaagaa aaatgggctg
    15121 agcgcattgc attactgcgt gattatcagg tgaacagcaa gatgatgcag ttgaccggta
    15181 acccggaggt caaattcctc cattgcctgc ccgcgtttca tgacgaccaa acgacgcttg
    15241 gcaaaaaaat ggcggaagag tttggcctac atggcggaat ggaagtgact gatgaggtct
    15301 tcgaatctgc cgccagcatt gtatttgatc aggcggaaaa ccgcatgcat accatcaaag
    15361 cggtgatggt cgcgacgctc agtaaattaa ataattaata tctctataaa
//
LOCUS       DAJTQY010000087        15343 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.087, whole genome shotgun sequence.
ACCESSION   DAJTQY010000087 DAJTQY010000000
VERSION     DAJTQY010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15343
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.087"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(173..1198)
                     /gene="tdh"
                     /locus_tag="OO349_002999"
     CDS             complement(173..1198)
                     /gene="tdh"
                     /locus_tag="OO349_002999"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HCR5867994.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNASKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(1208..2404)
                     /gene="kbl"
                     /locus_tag="OO349_003000"
     CDS             complement(1208..2404)
                     /gene="kbl"
                     /locus_tag="OO349_003000"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HCR5867995.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            complement(2679..3536)
                     /gene="yibB"
                     /locus_tag="OO349_003001"
     CDS             complement(2679..3536)
                     /gene="yibB"
                     /locus_tag="OO349_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HCR5867996.1"
                     /translation="MKSSTTIITAYFDIGRGDWTANKGFREKLARSVDVYFSYFERLA
                     ALENEMIIFTSPDLKPRIEAIRNGKPTTVIVIDIKKKFRYIRSRIEKIQKDESFTNRL
                     EPRQLKNPEYWSPEYVLVCNLKAYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLK
                     IWDFPFDENKMHLFTIKKGLTVTSQQQAFDFMIGNHVYIIGGAIVGSQHKWKEFYKLV
                     LESQKITLNNNIVDDDQGIFVMCYYKRSDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQ
                     ALRIFLSRK"
     gene            3840..4772
                     /gene="rfaD"
                     /locus_tag="OO349_003002"
     CDS             3840..4772
                     /gene="rfaD"
                     /locus_tag="OO349_003002"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HCR5867997.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            4782..5828
                     /gene="rfaF"
                     /locus_tag="OO349_003003"
     CDS             4782..5828
                     /gene="rfaF"
                     /locus_tag="OO349_003003"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HCR5867998.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            5832..6824
                     /gene="rfaC"
                     /locus_tag="OO349_003004"
     CDS             5832..6824
                     /gene="rfaC"
                     /locus_tag="OO349_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HCR5867999.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            6821..8029
                     /gene="rfaL"
                     /locus_tag="OO349_003005"
     CDS             6821..8029
                     /gene="rfaL"
                     /locus_tag="OO349_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HCR5868000.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEVFKAII
                     ENTLLCTIAIPVILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVSIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMMLYCAILKECIKNGVKNKYRSPYNAYYIILLSFIGYFVIRGNVEQIEPNLLGVY
                     AGLLLAMKNK"
     gene            complement(8065..9207)
                     /locus_tag="OO349_003006"
     CDS             complement(8065..9207)
                     /locus_tag="OO349_003006"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HCR5868001.1"
                     /translation="MVDKIIFTVTPIFSIPPRGAAAVETWMYQVAQRTNFPNRIVCIK
                     NPGYSNYTFVNDNCSIHRVGFSRIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESV
                     IVVHNSMKLYTQIRKLAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLAN
                     ADIEIVPNGIDLETYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKL
                     AAAHKNLKLVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMYCYYPL
                     ADLVVIPSQFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSI
                     SSDILKTLANPELTAVAKQGQDFVFDHYSWDGVTQRFEEVIHNWFE"
     gene            complement(9216..10229)
                     /locus_tag="OO349_003007"
     CDS             complement(9216..10229)
                     /locus_tag="OO349_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--(galactosyl) LPS
                     alpha1,2-glucosyltransferase"
                     /protein_id="HCR5868002.1"
                     /translation="MDFKHLTQFKDIIELDKRPVKLDERETFNVSWGIDENYQVGAAI
                     SIASILEKNKQNKFTFHIIADYLDKEYIELLSQLATKYQTVIKLYHIDSEPLKALPQS
                     NIWPVSIYYRLLSFDYFSARLDSLLYLDADIVCKGSLNELIALEFKDEYGAVVIDVDA
                     MQSKSAERLCNEDFNGSYFNSGVMYINLREWLKQRLTEKFFDLLSDESIIKKLKYPDQ
                     DILNLMFLHHAKILPRKYNCIYTIKSEFEEKNSEYYTRFINDDTVFIHYTGITKPWHD
                     WANYASADYFRNIYNISPWRNIPYKKAVKKHEHKEKYKHLLYQKKFLDGVFTAIKYNV
                     MKG"
     gene            complement(10254..10961)
                     /gene="rfaY"
                     /locus_tag="OO349_003008"
     CDS             complement(10254..10961)
                     /gene="rfaY"
                     /locus_tag="OO349_003008"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HCR5868003.1"
                     /translation="MIYNKTINGLKVFIKDNDPFYEQVLNDFLTCRVKTLKVFRSIDD
                     TKVILIDTARGPLVLKVYAPKHKMTERFLKSCIKKDYYENLIYQTDRVRGEGIQSIND
                     YFLLAERKTLNFAHYYIMLIEYIEGVGLNEYLEISEDLKDQLSESIKELHQHGMVSGD
                     PHKGNFIVSEKGLRLIDLSGKKTTAVLKAKDRIDLERHYNIKNELKDFGYTYLIFKKK
                     IKKVIRDVKVKLGLKSK"
     gene            complement(10987..11994)
                     /gene="waaO"
                     /locus_tag="OO349_003009"
                     /gene_synonym="rfaI"
     CDS             complement(10987..11994)
                     /gene="waaO"
                     /locus_tag="OO349_003009"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HCR5868004.1"
                     /translation="MSQLNDSDIILFEYNFHYQNIRSKNTLDIAFGIDRNFLFGCGVA
                     IASILLNNREISCEFHVFTDYISDKDKLYFSDLAKQYNSRINIYVINCDKLKSLPSTK
                     NWTYATYFRFIIADYFYHKHEKILYLDADIACKGSIKELLDYQFSTNEIAAVVAERDI
                     EWWQNRASVLTTPQLASGYFNAGFLLINIDEWNLNNISSKAIEMLRDPDWVSKITHLD
                     QDVLNVLLNGKVKFISEKYNTRYSINYELKDKVDNPVNDDTVFIHYVGPTKPWHEWAD
                     YPVSRSFLIAKAASPWSKEDLLKPVNSNQYRYCAKHKFKQKHYMAGIFNYLKYYKEKC
                     F"
     gene            complement(12037..12834)
                     /gene="rfaP"
                     /locus_tag="OO349_003010"
     CDS             complement(12037..12834)
                     /gene="rfaP"
                     /locus_tag="OO349_003010"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HCR5868005.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPGRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            complement(12827..13951)
                     /locus_tag="OO349_003011"
     CDS             complement(12827..13951)
                     /locus_tag="OO349_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCR5868006.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFEVIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGADRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            complement(13948..15006)
                     /gene="rfaQ"
                     /locus_tag="OO349_003012"
     CDS             complement(13948..15006)
                     /gene="rfaQ"
                     /locus_tag="OO349_003012"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HCR5868007.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
BASE COUNT         4377 a         3446 c         3146 g         4374 t
ORIGIN      
        1 ctgtgagata ttatttttac aggtgagaga gtcacttcag ggtagagaga taatgagaac
       61 agctatataa aagatcagcg caattacgaa ctctttatta cactgacagg attttacata
      121 attaacagat tatcctcaga taaaagggct ggaataccag cccttgttcg tgttaatccc
      181 agctcagaat aactttcccg gactggcccg aacgcatagc gtcaaagccc ttctggaaat
      241 catcgataga gaaacgatgg gtaatgatcg gcgagagatc gaggccagac tgaatcagcg
      301 ccgccatctt gtaccaggtt tcaaacatct cacgaccgta aatgccttta atgaacaagc
      361 ctttaaagat cactttggtc cagtcgatag acatatcaga cggtggaata cccagcatcg
      421 caatacgacc gccgtggttc atggtatcga gcatggtacg aaacgctggc ggcgcgccgg
      481 acatttccag accgacatca aagccttcgg tcatgcccag ttcagccatc acatcattaa
      541 ggttttcttt cgcgacgtta accgcacggg tgatacccat tttgcgcgcc agctcaaggc
      601 ggtattcgtt aacatcggtg atcaccacat tgcgtgcacc aacgtgtttc gccaccgccg
      661 cagccataat acctatcgga cctgcaccag aaaccagcac atcttcgccc accagatcga
      721 acgacagcgc ggtatgcacg gcgttaccga agggatcaaa aatggaagcc aggtcgtcgg
      781 agatattgtc ggggattttg gaagcgttga acgccggaat caccagatat tcggcgaagc
      841 agcccggacg gttaacaccg acaccaatgg tgttgcggca cagatgggta cgaccgccac
      901 gacagttgcg acagtgaccg caggtgatat ggccttcgcc agaaacgcga tcgccgattt
      961 tgaagccttt cacttcctga ccaataccta ccacttcacc gacatattca tggcccacga
     1021 ccatcggcac cgggatggtt ttttgcgacc actcatccca gttatagatg tgaacgtcag
     1081 tcccgcagat ggctgtttta cggattttaa tcagcagatc gttatgcccg agttccggta
     1141 cagggacgtc ggtcatccag atgccctctt ccgctttcag tttggataac gctttcatct
     1201 cacatcctca ggcgataacg cccagttgtt taccaatacg cgtgaatgct tctactgcac
     1261 gcgtaatttg ctcaggggta tgcgccgcag acatctgggt acgaatacgc gcctgacctt
     1321 tcggaacgac cggatagaag aaaccggtga cgtaaatgcc ctctttttgc agctctcggg
     1381 caaatttctg cgccactacc gcatcaccaa gcatgaccgg aataatggcg tgatcggctc
     1441 ccgccagggt aaagcccgcc gccgacattt gctcacggaa ctgacgcgcg ttcgcccaca
     1501 gacggtcacg caactcgcta cccgcttcaa ccatctccag cactttgatg gaagcggcga
     1561 caattgctgg tgccagcgag ttggagaaca ggtacggacg agaacgctgc cgcagccact
     1621 caaccacttc tttgcgcgcc gcggtataac caccagaagc cccgcccagc gctttaccga
     1681 gcgtgccggt gatgatgtcg acacgcccca tcacatcgca gtattcatgg gaaccacgac
     1741 cattttcacc gacaaaaccg accgcgtggg agtcgtctac catcaccagg gcatcgtatt
     1801 tatctgccag atcgcaaacg cccttcagat tggcaatcac gccgtccatc gagaacacac
     1861 catcggtggc aatcagcaca tgacgcgcac cggcttcacg cgcttctttc agacgagctt
     1921 caagctcctg catatcgttg ttggcatagc gatagcgttt agctttacac agacgcacac
     1981 catcaataat agacgcgtgg ttcagtgcgt cggagataat ggcgtcttcc gcgcccagca
     2041 gcgtttcaaa caggccaccg ttagcatcaa agcaggaaga gtagagaatc gcatcttcca
     2101 tccccaggaa tgccgccagt ttttgttcaa gctctttatg gctgtcctga gtgccgcaaa
     2161 taaaacgcac cgaagccatg ccgaaaccgt gagaatccat tcccgccttt gctgccgcaa
     2221 tcagatccgg atgattcgcc agcccgagat agttgttggc acaaaagtta atgacgtggc
     2281 ttccatcagc cacggtgata tctgcttgct gcgcagacgt aataatgcgc tcttctttaa
     2341 acaacccttc cgcccgtgcg gtttccagat cgttggttaa ctgctgataa aattctccac
     2401 gcattgcgat tctccagaca gggcaaattc cagcacatat tacccaaact tataggtcgc
     2461 gacgagatga tgcgttaaca cttctgcaaa attcaggata aataacgtgt atcttgatgt
     2521 ctctcaacac ctacagatag tagaaaaact gtacagtttt ggctgagcat aatgatgagt
     2581 gattttactg cattgatata aaaagaaaaa atgatgccaa agcatcattt ttcctatctc
     2641 agttggcatc aaaacgctac ccactaatgg agggaaacct attttcttga aaggaaaatt
     2701 ctcagcgcct gcatttttgc ccctaaagtg ttactcctga agcagcgaaa caaatcgaac
     2761 cattttcctc tccccagata gtttaggttg aagagatcgg aacgtttgta atagcacatg
     2821 acaaaaatgc cctggtcatc gtccacaatg ttattgttta gtgttatttt ttgactttcc
     2881 agaactaatt tatagaattc tttccattta tgctgtgaac caacaattgc gcccccaata
     2941 atatatacat gattgccaat catgaaatca aaagcctgtt gttgagatgt aacagtaagc
     3001 ccttttttta tagtgaaaag atgcatctta ttttcatcaa atgggaaatc ccaaattttc
     3061 aatcctcgag ttacatttgg cttacggcaa tagccaaaat ctatccacgc aacaagaggc
     3121 gttttcacta aacccatgtt gatagcttta tttacaaaat aggccttaag gttacatacc
     3181 aaaacatact ctggtgacca gtactctggg ttttttaatt gtcgaggctc aagtctattt
     3241 gtaaatgact catctttttg aattttttcg atacgacttc tgatatatct aaatttcttt
     3301 tttatatcga taacgataac cgttgttggt tttccgttgc gaatcgcttc aatccttggt
     3361 ttcaaatcgg gagaagtgaa aataatcatc tcattttcaa gtgcggccag acgctcaaag
     3421 taactaaaat aaacatcaac tgaacgagct agtttttcac ggaacccttt attagcagtc
     3481 caatcccctc taccaatgtc aaaatatgca gtgataattg tggtggatga tttcattact
     3541 aataagacca ttaatataaa aaaagaatta tatcataaat attaacagaa tgtatactgt
     3601 atattatcca ttcaaatgat tccgttattt atcatacatc ggaatattga tactaaagca
     3661 ctattcacat gcaaaaccaa catccgccat gaaggactag ctaaaaccca aactagtttg
     3721 ttgcaattag catccttgca cctctatgta aagggctgaa gggattcgga tgtgatggta
     3781 tgattacaga cattcgtgtc tgagattgtc tctgactcca taattcgaag gttacagtta
     3841 tgatcatcgt taccggcggc gcgggcttta tcggcagcaa catcgttaag gccctgaatg
     3901 ataaaggcat caccgatatt ctggtggtgg acaacctgaa agacggcacc aagtttgtga
     3961 acctggtgga tctggatatc gcggactata tggataagga agacttcctg atccagatta
     4021 tggctggcga agagttcggt gatgtcgaag cgattttcca cgaaggcgca tgctcttcca
     4081 ccaccgagtg ggatggcaag tatatgatgg ataacaacta tcaatactcc aaagagctgc
     4141 tgcactactg tctggagcgc gaaatcccgt tcctgtatgc ctcttccgca gccacctacg
     4201 gcggacgcac ctccgacttt attgaatccc gcgagtacga aaaaccgttg aatgtctacg
     4261 gttactcaaa attcctgttt gatgaatatg ttcgtcaaat cctgccagaa gcgaactcgc
     4321 agattgttgg cttccgctat ttcaacgttt atggaccgcg tgaaggccat aaaggcagca
     4381 tggcgagcgt cgctttccat ctcaacaccc agcttaataa cggtgaatca ccgaagctgt
     4441 tcgaaggtag cgagaacttc aaacgtgact tcgtctatgt gggcgacgtg gcagatgtaa
     4501 acctgtggtt cctggaaaat ggcgtttccg gtatcttcaa cctcggcacc ggtcgtgcag
     4561 aatccttcca ggcagtagca gatgctacgc tggcttatca caagaaaggc cagatcgaat
     4621 acattccgtt cccggataag ctgaaaggcc gctaccaggc gttcactcag gcagatctga
     4681 caaatctgcg cgcggcgggt tacgacaaac cgttcaaaac cgttgctgaa ggtgtaacgg
     4741 aatacatggc ctggctgaat cgcgacgcat aagagctctg catgaaaata ctggtgatcg
     4801 gcccgtcttg ggttggcgac atgatgatgt cgcaaagtct ctatcgcacg ctccaggcgc
     4861 gctatcccca ggcgataatc gatgtgatgg caccggcatg gtgccgtcca ttattatcgc
     4921 ggatgccgga agttaacgaa gctatcccta tgcctctcgg tcacggagcg ctggaaatcg
     4981 gcgaacgccg caaactgggt catagcctgc gtgaaaagcg ctacgaccgc gcctacgtct
     5041 taccaaactc cttcaaatct gcattagtgc ctttcttcgc gggtattcct catcgcaccg
     5101 gctggcgcgg cgagatgcgc tacggtttac tcaacgatgt acgcgtgctc gataaagaag
     5161 cctggccgct aatggtggaa cgctatgtcg cgctggccta tgacaaaggc attatgcgta
     5221 ccgcacaaga tctgccgcag ccattgttat ggccgcagtt gcaggtgagc gaaggtgaaa
     5281 aatcatatac ctgtaatcaa ttttcgcttt catcagaacg tccgatgatt ggcttttgcc
     5341 cgggtgcgga gtttggtccg gcaaaacgct ggccacacta ccactatgcg gaactggcaa
     5401 agcagctgat tgatgaaggt tatcaggtgg ttctgtttgg ctctgcgaaa gatcatgaag
     5461 cgggcaatga gattcttgcc gctttgaata ccgagcagca ggcatggtgt cggaacctgg
     5521 cgggggaaac acagcttgat caagcggtta tcttgattgc agcctgtaaa gccattgtca
     5581 ctaacgattc tggcctgatg cacgttgcgg cggcgctcaa tcgtccgctg gttgccctgt
     5641 atggtccaag tagcccggac ttcacaccgc cgctatccca taaagcgcgc gtgatccgtc
     5701 tgattaccgg ctatcacaaa gtgcgtaaag gtgacgctgc ggagggttat caccagagct
     5761 tgatcgacat tactccccag cgcgtactgg aagaactcaa cgcgctattg ttacaagagg
     5821 aagcctgacg gatgcgggtt ttgatcgtta aaacatcgtc gatgggcgat gttctccata
     5881 cgttgcccgc actcactgat gcccagcagg caatcccagg gattaagttt gactgggtgg
     5941 tggaagaagg gttcgcacag attccttcct ggcacgctgc cgttgagcga gttattcctg
     6001 tggcaatacg tcgctggcgt aaagcctggt tctcggcccc cataaaagct gaacgcaaag
     6061 cgtttcgtga agcgctacaa gcagagaact atgacgcagt tatcgacgct caggggctgg
     6121 taaaaagcgc ggcactggtg acacgtctgg cgcatggcgt aaagcatgga ttggactggc
     6181 aaaccgctcg cgaaccttta gccagcctgt tttacaattg taagcatcat attgcaaaac
     6241 agcagcacgc cgtagaacgc acccgcgaac tgtttgctaa aagtttgggc tatagcaaac
     6301 cgcaaaccca gggcgattat gctatcgcac agcattttct gacgaacctg cctacagatg
     6361 ctggcgaata tgccgtattt cttcatgcga cgacccgtga tgataaacac tggccggaag
     6421 aacactggcg agaattgatt ggtttactgg ctgattcagg aatacggatt aaacttccgt
     6481 ggggcgcgcc gcatgaggaa gaacgggcga aacgactggc ggaaggattt gcttatgttg
     6541 aagtattgcc gaagatgagt ctggaaggcg ttgcccgcgt gctggccggg gctaaatttg
     6601 tagtgtcggt ggatacgggg ttaagccatt taacggcggc actggataga cccaatatca
     6661 cggtttatgg accaaccgat ccgggattaa ttggtgggta tgggaagaat caagtagagt
     6721 gtcgttctac aagtatgtct cttgcagatt tgccagctca aaccgttttt caaaatttaa
     6781 atcttgaaat aataaccaat aagttgacat cggagataag atgacctcaa cattattttt
     6841 ctctctcgag aaaaaaaact ggatagcgta ctggaacaga gctctcgtat tcttattcat
     6901 taccacctat tttttgggtg ggattacaag gtataaacat cttattgtta ttcttatgac
     6961 aataacgaca atcgtctatc tctgcaaacg gccaaaacac tatctctcac tatttaaaac
     7021 atttcttttt ggtagtgttg ccatattaac tattgctgca ttgctgtcac ttcttcaatc
     7081 ccctgatgca ggtgctagca tgaaggaagt tttcaaagct attattgaga atactttact
     7141 atgcacaata gcaataccgg tcatattgag agacgagaaa agagaagatg tcgaaaaaat
     7201 cgttttcttc tcatttatta gtgcgttggg cttacgctgt ttttctgaat tgattaccta
     7261 ttataaggac tatcaacaag ggataatgcc attcgcagat tatagacacc gtagcatttc
     7321 tgactcgatg gtctttttat tccctgcatt gttaaatctc tggcttatca aatcagcaaa
     7381 ataccgcatt tcttttgtgg ttctaagcgt tatttttatt tttctgatat taggaacttt
     7441 atccagaggg gcctggcttt ccgtgttagt cattggatta atatggattc tgatgtttaa
     7501 acaatggaag ttactattag taggagtaat ggtttccatc attgcattgt cggttatttt
     7561 cacacataag gagatgaccg caaagctaac gtataaactt caacaaacta atagttctta
     7621 tcgctatgca aatggtactc aaggcagcgc actcgatcta atattagaaa atcctgttat
     7681 tggttatggt tacggtaacg ttgcatataa agatgtctat aataaacgtg tcattgatta
     7741 tccagaatgg acctttagac aatcaatagg gccacataat tttgcgctat tcatctggtt
     7801 tggcactggt ttattagggc tggtaagtct tatgatgcta tactgtgcaa tattgaaaga
     7861 gtgtataaaa aatggcgtca agaataaata tcgctcacca tataatgcat attatataat
     7921 cttactatct tttataggtt attttgttat ccgtggaaac gtagaacaaa ttgaaccaaa
     7981 tttattaggc gtttacgccg gcttattatt agcgatgaaa aacaagtaaa aaataaaaaa
     8041 ggctgcataa tgcggccttt ttatttattc aaaccaatta tgaataacct cttcgaatct
     8101 ttgagttacg ccatcccaac tgtagtgatc aaacacaaag tcctgacctt gctttgcaac
     8161 tgctgttaat tcaggattcg ctaacgtttt taaaatatcg ctgcttatgg agtctgcagt
     8221 cataggttct tttaaatgga atcccgtagt attttctttg acaaattccg tcatcccccc
     8281 acgcgtactc actaaaacag gcttaccagc acccatagct tcaattgcca ccatgcaaaa
     8341 tggttcctgg aactgggaag gaatgacgac taaatctgcc agtgggtaat aacaatacat
     8401 cttttcaggc ggtatgcttc ctaacataat gcaacgatcc ttcaaacgtt ttgcaatttc
     8461 cctgacattg cgttgatatg cacctttgtc acttttactc atttctgtat aatcaccaat
     8521 cacaactaat tttaaatttt tgtgagcagc ggccaatttt tcgaatgatt gcatcaacag
     8581 caatatgcct ttgtcaggga cgatacgccc agcataaaaa ataattttct cttcaggaga
     8641 gatattaagt tcgcttctag tgatcggctg aaaattagat tgataggttt ctaaatcaat
     8701 tccatttggt acaatctcaa tatctgcatt tgctaaataa gattggtaat actttttcaa
     8761 gtataaacta gggactatca ttttaacatt ttgctcaagc aattttggtt caaatgcatt
     8821 atgcatatgt ataaccactc ttgcctgagg agctagctta cgaatttgag tgtatagctt
     8881 catgctatta tgaactacaa taacactttc tttcgttata ggaaaatcat gtgcaatatt
     8941 caatattctc tgtgaatatg gaagaggatc aagtcttgtc catttttgaa ataaccgctt
     9001 atatatgcga ctgaatccaa ccctatgaat actacaatta tcattaacaa aggtgtaatt
     9061 tgagtatcct ggatttttga tacaaacaat acgatttgga aaattggtac gctgagcaac
     9121 ctgatacatc cacgtttcaa cagcagccgc tccgcgagga ggaattgaaa aaataggagt
     9181 aaccgtaaat attattttat caaccatctc gtatattaac ctttcataac attatattta
     9241 attgctgtaa aaacaccatc gagaaatttt ttctggtaaa gcaagtgttt atatttttct
     9301 ttgtgctcat gttttttaac agctttttta taaggtatat ttctccatgg tgatatatta
     9361 taaatattac gaaaataatc tgcagaggcg tagttcgccc aatcatgcca tggcttagtt
     9421 ataccagtat aatgtatgaa gacagtgtca tcattaataa accgggtgta atattcacta
     9481 tttttttctt caaattctga ctttatagta taaatacaat tatattttct cggtaatatt
     9541 ttagcatgat gtagaaacat taagtttaaa atatcttgat ccgggtactt taattttttt
     9601 ataattgact catctgataa tagatcaaag aatttttccg ttagtctttg ttttaaccat
     9661 tcccgtaaat taatatacat tacaccagag ttaaaatagc taccgttaaa atcctcatta
     9721 cacaaacgct cagcgctttt actttgcata gcatctacat caattaccac tgccccatat
     9781 tcatctttaa actctaatgc tattaactcg ttcaatgaac ccttacagac gatatcagca
     9841 tcaagatata ataatgaatc caatcgcgca gaaaaataat caaatgaaag caaacgataa
     9901 taaatagata ctggccagat atttgattga ggtagcgcct tcaatggctc agaatcaata
     9961 tgatataatt taattactgt ttgatacttc gttgctaatt gtgataataa ttcaatatac
    10021 tctttgtcta agtaatcagc gattatgtga aaggtaaatt tgttttgttt attcttttca
    10081 agaattgaag caattgaaat cgcagcccca acctggtagt tctcatcaat accccatgag
    10141 acattaaacg tttcccgttc atcaagttta acggggcgct tgtccagttc aattatatct
    10201 ttaaattgag taagatgttt aaaatccaat ttaattaccc ttcatcataa tcattatttg
    10261 ctcttcaatc ctagtttgac ttttacatcc ctgattactt ttttaatttt ctttttgaat
    10321 ataagatatg tatagccaaa gtctttcaat tcatttttta tattatagtg ccgttcaaga
    10381 tcaatgcgat cttttgcttt taaaacagcg gttgtttttt tacctgaaag gtcaattagt
    10441 cttaaacctt tttcggatac tataaaattt cctttatgag gatcgccaga caccattcca
    10501 tgctgatgca actcttttat cgattctgat aattgatcct tcagatcttc agaaatttcc
    10561 aaatactcat ttaacccgac accttcaata tactcaatga gcataatata ataatgagcg
    10621 aaatttaatg ttttacgttc agctagcaaa aaatagtcat taattgattg gatgccttca
    10681 ccacgtaccc gatcggtttg ataaattaaa ttttcataat aatctttttt tatgcaagat
    10741 tttaagaaac gttcggtcat tttatgcttt ggcgcataaa ctttaagtac aagaggtcca
    10801 cgagcagtgt caataagaat gactttagta tcatcaatag aacgaaaaac ttttaatgtt
    10861 ttaaccctgc aagttaaaaa gtcatttaga acttgctcat aaaagggatc attatctttt
    10921 atgaaaactt tcaacccatt tatcgtttta ttatatatca taatgaatta ttttaacctt
    10981 aaatctttag aagcattttt ctttataata ctttaaataa ttaaaaatgc ctgccatata
    11041 atgcttttgt ttaaatttat gttttgcaca ataccgatac tgattgctat ttacaggttt
    11101 aagtaaatct tctttactcc acggagaagc tgctttggcg atcaaaaaac tacgtgaaac
    11161 cggataatca gcccactcat gccagggttt tgtaggtccc acatagtgta taaacacggt
    11221 gtcatcattg actggattat caactttgtc ttttaattca tagttaatac tatatcgggt
    11281 attatatttc tccgaaataa acttcacttt accattcaat aatacattca gtacatcttg
    11341 atcaaggtgg gtgattttac ttacccaatc tgggtcacgc aacatttcaa tagcttttga
    11401 cgaaatgtta tttagattcc actcatcaat atttatcagt aaaaaaccag cattgaaata
    11461 tccagaagct aactgtggtg tagttaatac cgaggctcga ttttgccacc attcaatatc
    11521 tctttcagct acaactgcgg caatttcatt agtagaaaat tgataatcta agagttcttt
    11581 aatactaccc ttgcaagcaa tatctgcatc aagatatagt attttttcat gtttatgata
    11641 gaaataatct gcaattataa atcgaaaata tgttgcgtaa gtccagtttt tcgtgcttgg
    11701 taatgacttc agcttatcac aattgataac ataaatatta attcttgaat tatattgttt
    11761 tgctaaatca gaaaaatata atttgtcttt atcactgata taatctgtga aaacatgaaa
    11821 ttcacaagag atttctctat tgtttaatag aatagatgcg atggctacac cacatccaaa
    11881 aagaaaattt ctgtcaatac caaatgctat atcaagagta tttttagatc ttatattttg
    11941 ataatgaaaa ttatactcaa aaaggatgat gtcactatca ttgagttgag acatatttat
    12001 tctgtatcca aaattttggc aaaaataagt tacgggttac aacgattttc gaatcgttct
    12061 ttccctgatt tttgtggctt ttgcttctgc ttgcgacagc agtccttgtt cctgcttgag
    12121 aatgtcttta agcggggcgg caaaatacac cttcataaag cgccagatat cccgctgagt
    12181 caggccaata ttcattgaag aaaaataaag ccctataaga tctttatcgc gccaacgacc
    12241 tggaacgcgc gtgcgaagct gcgcccggtg taggtcaatt accgaaattt ttaactcctc
    12301 ttccttaccg gaaaaaggca agtgcagcag gaaatgacag atataacagt cacgatggtt
    12361 aatgcccgca gcatgcatat cgcgcaccat cgtcgcgaca cgcttaataa gcatacgctt
    12421 tacgcgaaca tctggtgggt tggtcgccca gtcagcacag taatcttcca gactgatggt
    12481 tggtgtcaga tcttcggtaa taataaacga ggttctggtc agcggattca tgcctttttc
    12541 gccgaatgcc accccataca tagtatcaac gccgacatcc cgcagtcgat gaatcgcatt
    12601 ccattcgcgg tctgcgccta atactggcat ccgcaatgag agtaaatttt tgattatctc
    12661 tttcagggtc gtgccgcgat gccatttgag aaaatagctt tttcccgcca tttcaaagcg
    12721 cagagtacgg cgagtttcca gttcacgaaa tacctcaccc tgcaaggttt taacttcctc
    12781 aaaaggatct ttgccgcgcc ataacgtggc aaacggctct ttaagttcaa ccatccagac
    12841 cacccgttat gatatccgcc gctttctctg gcagactgta taaatcttgt gtatcagcat
    12901 aatgtcgcgc attttccgcc caagcctggc gcaatgaaga ttgcgttaac gctttgcgta
    12961 aaatctcatt caatgtttcc tggcggaatg gctcagcaat agcctcgccg caattagcgt
    13021 cgacaatata atgcgcatag ccacaaacgg cagttgttag taccggtaat cctgcagtaa
    13081 tcgcttccag cagcacaatt cccgccgctt cctggtaggc aggatgcagt aataaatccg
    13141 ccgccgccat taattccgag acatcgttgc gccccgagaa gaagtgaaca ttactgcgca
    13201 cgccgcgttt ttctgccagt gcctcaaatt ttcgcggttt atcctgccca acaacatata
    13261 gcaatgtgtt gtggcgcagc gaatccggta acgaagcaag tgcttcaatg gaacgatcgg
    13321 cacctttacg cgtgaagtct gaaccgacct gcaacaataa atattgttgt tcggttattc
    13381 cattcttctt acggaagatt tcacggctat tggctggctg ctggctatat ttacgatcag
    13441 gataaatccc cggtggcaga atatgaaaac gctccgcttc agtctgataa tgtttctgga
    13501 aatcggcgat ttgcttatct gtcagcatca gcagctgtgt cggcttgccc tgttcgaagg
    13561 ttgcccgctc aaaggcggca taatggcgat aacgtgacgt caggcgatag aaaaagcctt
    13621 tttcctgcgc tactttctcg gcataacaaa catcagcggc ataataaacg tccagccccg
    13681 gcattttgtt gaatccaacg actttatcga cgggatgttc gcgtaaatgt ttttgcaccc
    13741 aggcaaaata ctccgcattg cgcccgtgat tggtatgcga tttaaccggc actttgatca
    13801 cttcaaatac atcagggcat tcgccttccc acgactgggt ataaacccga acatgatgac
    13861 ctcgggcggc gactgtctga gcaatacgca taaaatcgcg ctgcaaaccg ccaaagggaa
    13921 aatatttata taaacaaaaa gcaacgatca taacgacgta cctgtcgtgg aggagggcag
    13981 taatttatcg acagcagcaa tgacatctgc cgccggaata accgaaagat acatctcatt
    14041 tcggtcacgc tgatcgcgcg ttggcatttc ccggtaatct cccgcccaga attgaatcat
    14101 gttatttgac cagggacgcc agaaaatatg atctgttgcg ccaaacagcg atatcagcgg
    14161 cgtattaact gcagcggcaa tatgcgccgg tgcggaatca acgccaataa acagctgcgc
    14221 atgatcgatt aacgcaccaa gttccgggaa ggtcaccttt ccagccagcg ccgttactgg
    14281 tggcgtctgg catccctgcg caatttcatt gacgcaggcc agatcgtctt tatctgggcc
    14341 ggacgtcaga acgacttcat aaccacgagc atgtaaggca tcaatcacag cggaaaactt
    14401 ggcgttgtcc cagcatttga agatttgccg cgccgtaggt tggataacca cataattttg
    14461 tccaacacca gcgtgatcaa gttcgcgacg catacgtttc cagcttgcag gcgggtaact
    14521 catggttgtc tgcttcacca acgaatcaag tcccaatggt gtcagcacgg ataagttact
    14581 ttccaccaca tttccaccct gcaacggcac caaatgggtg aaacttttac gccaaaaagc
    14641 agactgccga tgatgataat cctgggaaat tttcacacga gcatttaata agcgaaccag
    14701 tatagcaacc atccattgat cggtaagatt gacgataagg tcatacttat tggcacgtaa
    14761 taccttgatg agatgaaaaa agttggcaat tttttctgag gcttttgctt ttttattttt
    14821 tatgccgtag agcgcgttaa tctctggatt ttcagacagg atcgggatgg tgtcctgata
    14881 aagcagcaca tcgatttttg cgtcagggta attttttttc agagaactaa tgacgggagt
    14941 agttaataac atatccccat gaaaacgcat cttaatgagc aaaattcttc gaaatggctt
    15001 atccacaagt aactcttttt gttgagatta tccgattaag gtgaagcaga aaaagcacgc
    15061 taccgcccca ggctcaacag ctacctgaat actgataccg cactgatatt gacgcgctag
    15121 tgtatcactt ctttcgcggc aatccgaccc gaatgatttt taagcacatt gttgattttc
    15181 aataaagaaa tcaatcaaat aaaatcatta gcaaccatat tctttcactg ttgctctgcg
    15241 attccttcct gcacgtacag cagaaatcaa tgaatcgcat aaaaatacaa aaaaagtata
    15301 gatgttcagc aagaattggc gggtaaattg ctatttaagt caa
//
LOCUS       DAJTQY010000088        15102 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.088, whole genome shotgun sequence.
ACCESSION   DAJTQY010000088 DAJTQY010000000
VERSION     DAJTQY010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15102
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.088"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            <1..181
                     /gene="yjbD"
                     /locus_tag="OO349_003013"
     CDS             <1..181
                     /gene="yjbD"
                     /locus_tag="OO349_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HCR5868008.1"
                     /translation="RVLTNSETNSIKKEYIDKLMVEREAEAKKARQLKKKQAYKPDPE
                     ASFSWSANTSTRGRR"
     gene            complement(314..1186)
                     /gene="rluF"
                     /locus_tag="OO349_003014"
     CDS             complement(314..1186)
                     /gene="rluF"
                     /locus_tag="OO349_003014"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HCR5868009.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRYLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            1534..2481
                     /locus_tag="OO349_003015"
     CDS             1534..2481
                     /locus_tag="OO349_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HCR5868010.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGMVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDIALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIIGALRGKYINCLVTNSSTAELLLK"
     gene            2551..3357
                     /locus_tag="OO349_003016"
     CDS             2551..3357
                     /locus_tag="OO349_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5868011.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSTKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            3367..3774
                     /locus_tag="OO349_003017"
                     /pseudo
     CDS             3367..3774
                     /locus_tag="OO349_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709810.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIA"
     gene            3774..4268
                     /locus_tag="OO349_003018"
     CDS             3774..4268
                     /locus_tag="OO349_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HCR5868012.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            4334..5131
                     /locus_tag="OO349_003019"
     CDS             4334..5131
                     /locus_tag="OO349_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HCR5868013.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALCWMNVGAAQSPDSALASIISAILVIVGQQNIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQLQATASTALDQLDD"
     gene            5142..5930
                     /locus_tag="OO349_003020"
     CDS             5142..5930
                     /locus_tag="OO349_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCR5868014.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRLHLVFFNTTPAVCGPVIGAEIDDGTINGIKVGLMGPLAGVGDPL
                     VWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQLGFRKGVNIVSDM
                     GGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGSTVTMTVQNILDQL
                     CPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            5982..7211
                     /gene="sorE"
                     /locus_tag="OO349_003021"
     CDS             5982..7211
                     /gene="sorE"
                     /locus_tag="OO349_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HCR5868015.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVTTNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVDGEATHVVIPNEVMEQYCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNATDAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLAHAEAISHD"
     gene            7593..8282
                     /gene="pepE"
                     /locus_tag="OO349_003022"
     CDS             7593..8282
                     /gene="pepE"
                     /locus_tag="OO349_003022"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HCR5868016.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTEKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVLLEAGHRF"
     gene            complement(8373..10004)
                     /locus_tag="OO349_003023"
     CDS             complement(8373..10004)
                     /locus_tag="OO349_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HCR5868017.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(10224..13907)
                     /gene="metH"
                     /locus_tag="OO349_003024"
     CDS             complement(10224..13907)
                     /gene="metH"
                     /locus_tag="OO349_003024"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HCR5868018.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLA
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGDDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKADDTANAQQAEWRSWDVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRIAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFAIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTCKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            14107..14931
                     /gene="iclR"
                     /locus_tag="OO349_003025"
     CDS             14107..14931
                     /gene="iclR"
                     /locus_tag="OO349_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HCR5868019.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELSQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
BASE COUNT         3592 a         3982 c         3936 g         3592 t
ORIGIN      
        1 tcgcgtactg accaactccg aaaccaacag cattaagaaa gagtacatcg ataaattaat
       61 ggtcgagcgt gaggctgaag cgaaaaaagc ccgccaattg aagaaaaagc aggcttataa
      121 accggatcct gaagcgtcat ttagctggtc agcaaatacg tcgacgcgcg gcaggcgcta
      181 atgaacaatc aacgcctgag attttcactc gtctgtgtgg acacaatctt cccctcaccc
      241 taaccctctc cccgaagggg cgaggggact gtccgagctc tttttggacg ttttcatcag
      301 tctgaggcgt taatcagcgt cccttctttt tacgccccgg cgaggtaaaa cgcttaccgt
      361 tggacgccgg gcgaccgcct ttttccgccg ttttttccat cttaacgact ggacgtttga
      421 tgcccgctgt tttcggtttg gccttcgctt taggttttgc ctcggaagag gaattttcga
      481 tgagcttaaa gaggtcgatt aactcatcgt cggttaaata gcgccactcc cccagcggaa
      541 tgccgcttaa gctgacgttc ataatgcgcg tacgttccag ctttttcact tcatagccga
      601 aatgctcgca catgcgccgg atctgacggt tcagcccctg cactagggta atgcggaaga
      661 caaacggcgc ttcttttttg actttgcact ttttcgtcac cgtcccgagg atcggcaccc
      721 ccgcccccat accacgaata aattcatcgg taatcggttt atcgaccgtc accagatact
      781 ctttctcatg atcgttaccc gcacggagga tcttattcac cagatcgccg tgattggtga
      841 ggaaaatcag cccctgggag tctttatcca ggcggccaat cgggaacacg cgtttgctgt
      901 ggttaacgaa atcgacaatg ttatcgcgct cgccatcttc ggtggtgctg acaataccga
      961 cgggcttgtt cagggcgata agtaccaaat cttcggcttc ccgaggctca atcaactgac
     1021 cgtttacttt cacaatgtcg ccgggtttca cctgatcgcc aatggtggct cgcttgccat
     1081 taaggaacac attgccttgc tcgatatagc gatccgcttc gcggcgtgag caaattccgc
     1141 tttcgctgat gtatttattt aaacggactg atgagtcggg cagcataaat tctcctgtaa
     1201 aacgcggaat ataccgtacc tcttagcggg gaaaaacagg gttatgcggc aacgtcgaaa
     1261 ttaaatgtga tctgccacgc cttttatcac aaaagtgtct ccggaagtac gatgcaaatt
     1321 tgcctggcaa aataaggata atcaacatat tacgaatcgc tgcacacctg tgctgatgac
     1381 tactacgcag agaaacaaat gtgcaagaga aagcgctatt tctccccatg caaagagtga
     1441 aaaatcgctt tgaaaggccc tcatgatggg gttatatcag atcaagcggt gagaaagcgt
     1501 atttgcacaa atgtgcagaa ccaggaggcg gcaatggaaa acagtgacga tatccgtttg
     1561 attgtgaaga ttgcccaact ctattacgaa caggatatga cgcaggcgca aatcgcgcgc
     1621 gaactgggta tttaccgcac caccatcagc cgcttgctta aacgaggccg cgatcaggga
     1681 atggtcacca tcgccatcaa ctatgactac aacgaaaatc tctggctgga gcagcaactg
     1741 aagcaaaagt ttggcctgaa agacgttgtg gtggtgtcgg gaaatgatga ggatgaagag
     1801 actcaactgg cgatgatggg gttacacggc gcgcaactgc tggatcgctt gctggagcct
     1861 ggcgatattg tcggtttttc ctggggtcgc gcggtgagcg cactggttga aaacttgccg
     1921 caggcggggc aatcgcggca gttaatctgc gtaccaatta ttggcggacc gtccggtaaa
     1981 ctcgaaagcc gctatcatgt aaacacatta acctacagcg cggcagcgaa gctgaaaggg
     2041 gaatcgcatc tcgcggattt tccggctctg ctggataacc cattaattcg taatgggatc
     2101 atgcagtctc agcactttaa aaccatctct gcctactggg ataatctgga tatcgccctg
     2161 gtgggaattg gctcaccggc cattcgcgac ggcgctaact ggcatgcgtt ttatggtggt
     2221 gaagagagtg acgacctgaa tgcccgccag gttgctggcg atatttgctc gcgctttttt
     2281 gatattcacg gcgcaatggt tgaaacgaat atgagcgaaa aaacgctctc tatcgaaatg
     2341 aataaattaa agcaggcgcg atattccatt ggcattgcca tgagtgaaga aaaatacagc
     2401 ggaattattg gtgcactgcg tggaaaatat attaattgtc tggtaacgaa tagcagcaca
     2461 gctgaactat tactgaaata aaaatcttcc cgatgtttac cgtaataaaa attacggcgg
     2521 gtccctatta tctaaaatca ggagtctgtt atgcaaacgt ggttaaattt gcaggataaa
     2581 atcattattg tcaccggcgg cgcatccggt attggtctgg cgattgtaga ggaattatta
     2641 gcacaaggcg cgaatgtaca gatggtcgat attcacggtg gcgatggtca atatgaaggt
     2701 cataaaggct atcagttttg gccgaccgat atttctagca caaaagaggt aaatcatacg
     2761 gtagcggaaa ttatccagcg ttttggtcgc atcgacggtc tggtcaataa cgccggggtc
     2821 aatttcccgc gtctgctggt cgatgagaaa gcgcctgccg ggcagtatga actcaacgaa
     2881 gctgcattcg aaaaaatggt caatatcaac cagaaaggtg tttttctgat gtcgcaggcg
     2941 gtggcgcgac agatggtcaa acaacatgat ggcgtgattg tgaatgtttc ctcagaaagt
     3001 gggctggaag gctcagaagg acaaagctgt tacgccgcta ccaaagccgc gctcaatagc
     3061 ttcacgcgct cctggtcgaa agagctgggt aaacacggta tccgtgtggt cggtatcgcg
     3121 cccgggattc tggaaaaaac cgggctacgc acgccggaat atgaagaggc gctggcgtgg
     3181 acgcgcaata tcaccgtcga gcagctgcgt gaaggctata ccaaaaacgc cattcctatt
     3241 gggcgcgccg gaagattagc agaagtagct gattttgttt gttatctgtt gtctgaacgc
     3301 gccagctaca tcaccggagt aaccactaac attgcgggcg gcaaaacgcg cggctaagga
     3361 ggctttatgg tcaacgccat tttttgtgcc cacggcaaat tagcttgcgc catgctggaa
     3421 tcggttcaga tggtttacgg caacgccaat gttgaagccg ttgcatttgt gcccggtgaa
     3481 aacgccggag acattgcggt aaagctggag aagttagtaa gcattcacaa tcaagatgag
     3541 tggctgattg ccgtggattt acagtgcggc agcccgtaga atgcggcagc aatgcttgcg
     3601 atgcgcaatc ctcgtttgcg ggtaatcagc ggcctttctc tgccgctggc gctcgaactg
     3661 gttgataacc aggacagcat gaatgttgat gaactgtgtg aacacctgac gcaaatcgca
     3721 aagcaaacct gcgttgtctg gaaacaactg gcaacgacag aggaggattt ctgatgaaca
     3781 ttacgctcgc ccgcattgat gaccgtctga tccacggcca ggtcactacc gtctggtcga
     3841 aagtggctaa cgcccagcgc attattatct gcaatgacga agtttataac gatgaagtgc
     3901 gccggacatt attgcgtcag gctgcgccac cgggaatgaa ggttaacgtg gtcaatattg
     3961 aaaaagccgt cgcggtttat cacaacccgc aatatcagga cgaaaccgtt ttttatcttt
     4021 tcactcgccc acaggatgcc ttagcgatgg ttcgccaggg ggtaaaaata ggcaccctga
     4081 atattggcgg catggcctgg cggcccggta aaaaacagtt aaccaaagcg gtttcattag
     4141 atgatgatga tattaacgca tttcatgagt taaataatct tggtgtcata ctggatttac
     4201 gcgtcgtagc gtcagatccc tcaatcaata ttatcgacaa aataaacgaa cagttaatcg
     4261 caaattaaat aatagcgcct gtgtacatat gctttaacag gcatggatga cgacacttaa
     4321 aaggtgacaa atcatggaaa taagtaccct acaaatcatt gctatatttc ttttttcctg
     4381 tattgccgga atgggcagcg tgctggatga atttcagact catcgcccgt taattgcctg
     4441 tacggtgatt ggtttaattc tcggtgattt aaaaaccgga ataatactcg gcggtacgct
     4501 ggaattgata gctctctgct ggatgaacgt cggcgcggcg caatctccgg attctgcact
     4561 cgccagcata atctccgcca ttctggttat cgttggtcag caaaacatcg ccaccggaat
     4621 cgccatcgcg ttgcctgtgg ctgcggcagg ccaggtgctg acggtgtttg cccgtaccat
     4681 caccgtagtg ttccagcacg cggcggataa agcagcagaa gaggcgcggt ttcgcactct
     4741 cgatattctg catgtctccg cgcttggcgt gcaggcgctg cgcgtcgcca ttccggcact
     4801 gattgtctca ctgttcgtca gcgccgatat ggtcagcaat atgttaaacg ccattcccga
     4861 atttgtgacc cgaggactac agattgctgg cggttttatc gtggtggtcg gttacgccat
     4921 ggtgctacgt atgatgggcg tgaaatattt gatgcctttc tttttcctcg gtttcctcgc
     4981 aggtggctac ctcgatctca gcctgctggc gttcggtggc gtcggcgtga tcatggccct
     5041 ggtctacatc cagctaaatc cacagtggcg taaagctgaa cctcaactcc aggccaccgc
     5101 ctccaccgcc cttgaccaac ttgacgatta acggagccat catggaacag agaaaaatta
     5161 cacgcagcga tctggtgagc atgtttctgc gctccaacct gcaacaggcg tcctttaact
     5221 ttgaacgtat tcacgggctg ggcttttgct acgacatgat ccccgccatc aagcgacttt
     5281 acccattaaa agaggatcag gttgcggcgc tcaggctaca cctagtgttc ttcaatacca
     5341 cgccagccgt atgtggcccg gtcatcggcg cggaaattga tgacggtacc atcaacggca
     5401 tcaaagtcgg tctgatggga ccattggcag gagttggcga tccactggtc tggggaacgc
     5461 tgcgcccgat taccgccgcg ctcggcgcat ctctggcact ttcgggcaac attctcggcc
     5521 cgctgctgtt cttctttatt ttcaacgcgg tgcgtctggc gatgaagtgg tatggcctac
     5581 agctcggctt tcgcaaaggg gtgaatatcg tcagcgatat gggcgggaat gtgctgcaaa
     5641 aactcaccga aggcgcgtcg attctcggac tgtttgtgat gggcgtgctg gtgaccaaat
     5701 ggacgtcaat caacgtaccg ttggtggttt cacaaacgca tgccgccgat ggctccaccg
     5761 tcaccatgac cgtgcagaac attctcgacc aactttgccc tggtttgctg gcgctcggtc
     5821 tgacgctact aatggttcgt ctgctcaaca aaaaaattaa cccggtatgg ctgattttcg
     5881 ccctgtttgg cttagggatt atcggcaatg ctctgggctt cctgtcctga ttcttcgccc
     5941 cggcacgact gccgaggcca tcgctcaaca tgaggtggtt tatgaaaaca acagctctgc
     6001 gtctttatgg taaacgtgat ttacgcctgg aaacctttga ccttcctgaa atgcaggagg
     6061 atgaaatcct cgcaacggtg gtcactgaca gcctgtgcct ctcttcctgg aaagaggcca
     6121 atctgggtga aaaccataaa aaagtacccg acgatgtgac gaccaaccca atcatcatcg
     6181 gccacgaatt ttgcggcgat attctggccg tgggtaaaaa gtggcagcac aaattccagc
     6241 cgggtcagcg ttatgtgatt caggccaacc tgcaactccc cgaccgcccg gattgccccg
     6301 gctactcctt cccgtgggta gacggcgaag cgacgcatgt ggttattccc aacgaggtca
     6361 tggaacaata ttgcctgctg gcatacgacg gcgaaaccta ttttgaaggc tcactggttg
     6421 aaccgctttc ctgcgtgatt ggcgcgttca acgccaacta tcatcttcag gaaggtagtt
     6481 ataaccacac gatggggatt cgcccgcaag ggcgcacgct gatcctcggc ggcaccggac
     6541 caatgggact gttggcgatt gattatgcgc tgcatggacc agttaacccg tcgctgctcg
     6601 tcattaccga taccgacaac gacaaattga gttatgcgcg caagcactat ccatcagaac
     6661 cgcaaacact gattcattat ctcaatgcca ccgatgcagc atttgatacg ttaatggcgc
     6721 tgagtggcgg tcacggcttc gatgatattt tcgtctttgt gcctaatgaa ggactggtga
     6781 ctctcgcctc ttccttgctg gcgacagatg gttgcctgaa tttcttcgcc ggaccgcagg
     6841 ataaacattt cagcgcgcca attaatttct acgatgtgca ttatgcattt acccactacg
     6901 tgggcacgtc aggcggcaat accgacgaca tgcgcgcggc ggtcaaactg atcgaagaga
     6961 aaaaagtgca ggccgcaaaa gtggtaacac atattcttgg gctgaatgcc gcgggcgaaa
     7021 ccacgcttga attgcctgcc gtcggcggcg gcaaaaagct ggtgtatacc gggaaatacc
     7081 tgccgctgac gtcactcacg cagattcagg atcaagcact ggcggcgatt ctggcgcgtc
     7141 atcaggggat ctggtccggt gaggcggagc aatacctgct cgcccatgca gaggcaattt
     7201 cccatgatta atcgcgctac ttaactctgc atgtcgctgg ctggtcgccc cagcaacttc
     7261 gcgaccggat gtgcggctga ttgctgaagc agcacaattt gcacgagaag gataaaaaat
     7321 atcagcgcct taaggggcgc tcaaattggt ggcaaagtac acattgttta tgccggatgc
     7381 ggaatgaagc cttattcggt ctacataatt ttgaaaatcc aatatattgc agttatctgg
     7441 taggccaggt aagcgtagcg catcaggcaa tgctgtgttt gtcatcagtc tcagcgcccg
     7501 gtgcaatacg tgacatcgtg agccatgtca gtaagtggtt aaattcgcag tggtagcatg
     7561 ccttccgtga tttcttacta accggagaag taatggaact gcttttattg agtaactcga
     7621 cgctgccggg taaagcctgg ctggaacatg cactgccgct aattgctgaa cagttgcagg
     7681 gtcgccgctc agcggtgttt atccctttcg ctggcgtaac gcagacctgg gatgattaca
     7741 cagagaaaac ggctgcggtt ctcgctccgc tgggtgtttc tgtcaccggt attcatagcg
     7801 ttgtcgatcc cgttgccgcg attgaaaatg cggaaattgt gattgtcggc ggcggtaata
     7861 ctttccagtt gctgaaacag tgccgcgagc gcgggctgct ggctccaatt actgacgtgg
     7921 ttaaacgtgg cgcgctgtat attggctgga gcgcaggcgc taaccttgct tgcccaacca
     7981 ttcgtaccac caacgatatg ccgattgtcg atccgcaagg tttcgatgcg ctaaatctgt
     8041 tcccgttgca aatcaacccg cacttcacca acgcgctgcc ggaaggccat aaaggtgaaa
     8101 cccgtgagca gcgtattcgc gaactgctgg tcgtcgcgcc agaactgacg attattggtc
     8161 tgccggaagg taactggatc acagtgagta aaggtcacgc tacgctgggt ggcccgaaca
     8221 ccacttatgt gtttaaggct ggtgaagaag cggttctgct ggaagctggt caccgttttt
     8281 aagtgaattg ccggatgcat gatccggcat cttgttcata gtgcctgatg cgacgcttga
     8341 cgcgtcttat caggcctaca ggattcacaa tattaatact cgtcccgtcc ttcatcttca
     8401 tccggctgtt ccagcacact gtaagccacc gaacaaaaca gcgagttcag gcgctgcata
     8461 tctcccagta agcctaaatg tagcgaactg gtttcaatgc tctgtacgtt ttgctgatgc
     8521 aggcgatcga cgtgggcgtg ggaatagcgg cgattaagaa tgcgaaaacg atgtttgctg
     8581 cgacgcaaac gacgagcgct ggtgacatcg ccagagaaga acaccgacat tgccagcttt
     8641 aaattactga gcaattgctc atagagcgca tccagttcct tcaacccatc aagcgaaaat
     8701 gcccgccgtg ctgccagcga tttatcggca atttcgctgc ccatgcgctc gacgatatcg
     8761 gaggcctgtt caaggttgag cgacatttcg atgatctccg cccagcggcg cgactcttcc
     8821 tccgccaact cttcttttgg catccgcgcc agatacagct taatggcggt gtagagaacg
     8881 ttgatatcat ctgccagctt acgcagctct ttctcctgcc gtggctcgcc gtgcatcact
     8941 ttattcagcc cttccatcat ctgttccatg gcgtcgccaa tgcgcagggt ttcgcgcgcg
     9001 gcgttcgcca gagcaagcgt gggcgtgtcc agcgcgctga catccagatg tttaggccgt
     9061 agctgggtat ccagttccgg ttcatcgcga ataatcgttt tgcaaaaccg tgccatcggg
     9121 tcaacaaatg gcagcatgac caggcaacgt acaaggttgt agaagacgtg gaaatagatc
     9181 accagttccg cttttggcaa tgacagcttc cccattgtct ctgccagcaa atggacaaac
     9241 ggcaggataa tcaggctacc caccagctta aacagcagac tacccagcgc gacacggcgg
     9301 gctgcggcat tggcggcact gttgttgagc atcgccagca ggccggaacc gaggttagct
     9361 ccaatcacca gacagagcgc cacggggaag gagataatgc ctgcagcggt cagagtcgcg
     9421 gtcagcagta cagcagcaag gctggagtag ctgataatgg cgaacatcgc gccaatcagc
     9481 gcatccagca gaatatcgcc ggtcagcgag gcaaagatca cctgaacgcc gtttgcctgg
     9541 gtgatcggcg ttacggcctg cacaatcaac tccagcgcca gcaaaatcag cccaagacca
     9601 ataccgacgc ggcccagttg cccggcgcgt gactgtttgc gtccgaggaa aaaaatcacg
     9661 ccgataaaaa taagtaacgg tgacagccag gataagtcga aggtgagaat acgcgccatt
     9721 agcgccgtcc cgacatctgc ccccagcaca atgaccagag ccggtgcgag ggctaccaga
     9781 tcctgagcga cgaacgaggt caccagcatg gtggtggcat tactgctctg taccagtgcg
     9841 gtaacgccga tccccgcgca aaaggcgagc ggcttttttt cgacgctccg gctaaggaca
     9901 gtacgcaaac gcgcgccgaa gacgcgcatt acgccggttc gaacaatatg agtcccccag
     9961 accagcaggg cgacggcaga aagcaggtga agcagcgtta acacgagttc aggttctcct
    10021 tatcgttata tgttcctgaa gcaattgaat gaccaaagga ccggaaacac gctgactggc
    10081 atctttactg tgccttttga cattcgtttt tccggctaag attattccag cctgtttcca
    10141 gtataagtgt ttcatattaa taaagagaca gggcactcat taagcgccct gtttgttgta
    10201 aggaaaagtg acagatttgt gaatcagtcc gcgtcatacc ccagattcgg tgccagccag
    10261 cgctcaacat cggagacgct cataccttta cggcgggcat agtcttcaac ctgatcgcgc
    10321 tgaatttgcg ccaccgcgta gtacttgctg tccggatgac tgaaatacca acccgaaacc
    10381 gatgcaccag gccacatcgc gaaagattct gtgagtttca tgccagtatg tttttccact
    10441 tccagcagct cccagatggt ggctttttcc gtatgttccg ggcaggccgg atagcccggt
    10501 gccggacgga tgccctggta gttttcgcgg atcagttctt cgttgctgag gttctcgttc
    10561 ggcgcatagc cccagtagac tttacgcaca cgctcatgga gatactccgc aaaggcttcg
    10621 gctaaacggt cggcaagcgc tttcaccatg attttgttgt aatcatcgtg ctgcgcttca
    10681 aaggcatcag ccagtgcgtc ctcttccagc ccgccagtca cggcaaatgc gccgatgtaa
    10741 tctgctttac cagaaagctt cggcgcaacg aagtcagcga gacagtagtt agcgaatccc
    10801 gttttttcgg tctgttgacg cagatggtgg ctgacgttga tcacatgggt gcgcgtttcg
    10861 tcacggtaga tttcaatgtc atcgcccaca cggtttgccg ggaacaggcc caccacgcca
    10921 cgcggattca gcgttttctc ggcgcttaat ttatccagca tgtcgttggc gtctttaaac
    10981 agccgctgcg cctcaacgcc caccacttca tcttccagaa tgcgcggata cttcccggcc
    11041 agcgaccagg tcataaagaa cggtgtccag tcgatgtaat tacgcagcgt ttcgatgctg
    11101 gcttccactt cctgcacgcc gagacggtgc gccaccggcg gcgtgtaagc ctgccagtca
    11161 aatgcgaaat cgttatcgcg cgccgcttcc agcgtgaccg gtggtgtgcg tggtttcttg
    11221 cgcccgtgct gaatacgcac ggtttcgtac tccttgcagg tacgagcgac aaaatcatca
    11281 cgctgagtat cggaaagcag cgccgctacc acaccaacgg tgcgcgaggc attctgcaca
    11341 tacaccgtag ggccgctgta gttctgctcg attttcaccg ccgtgtgcgc ttttgaggtg
    11401 gtcgcgccgc caatcagtaa cggaatggcg aagccctgac gctccatctc tttcgccacg
    11461 ttaaccatct cgtccagcga cggcgtgata agccccgaaa ggccaatcaa atcagcattc
    11521 acttctttgg cgatacggag aattttttcc gcaggcacca taacgccgag atcgacaatt
    11581 tcgtagttgt tacattgcag caccacacca acgatatttt taccgatgtc gtggacatcg
    11641 cctttcacgg tggcgatcac catcttgccg ttggttttac cctgctcttt gctggcttca
    11701 ataaacggtt cgagatacgc cactgcctgt ttcatgacgc gcgccgattt gaccacctgt
    11761 ggcaggaaca ttttgccttc gccaaacaga tcgccgacca cgttcatgcc gtccatcaac
    11821 gggccttcaa tcacttcaat cgggcgcgta gcctgctggc gggcttcttc agtatcctgc
    11881 tcgataaact cggttatgcc tttgaccagc gagtattcca gacgtttatt cacgtcccac
    11941 gagcgccact ccgcttgctg ggcgttagca gtgtcgtccg ctttactgcc gcgatatttc
    12001 tcggcaagct ccagtaaacg ctcagtgcca tcgtcacgac gattaagaat cacatcttcc
    12061 accgcgtcgc gcagttcagc gggtaggtcg tcgtaaatcg ccagttgccc ggcgttgacg
    12121 atccccatat ccatgccatt gcgaatagcg tagtagagga acactgcgtg aatggcttcg
    12181 cgcaccggat cgttgccacg gaatgagaaa gaaacgttag atacgccgcc ggaaatcagc
    12241 gcgtgcggca gttcgcgttt gatgtcttca cacgcgccga taaagtcctg cgcgtagttg
    12301 ttgtgctctt caataccggt ggcgaccgcg aagatgttcg ggtcgaagat gatatcttct
    12361 ggcgggaagc caacctcttc ggtgaggatt ttgtacgccc gacggcaaat ctcgattttc
    12421 cgtgcgcgag tatcggcctg tccctgttcg tcaaaggcca tcaccaccac tgccgcaccg
    12481 tagcgacgca acaatttcgc gtgatggata aaggcgtcga cgccctcttt catcgagata
    12541 gagttaacaa tgcctttgcc ctggatacac ttcagacctt tttcaatgac atcccatttt
    12601 gaggagtcga tcataatcgg cacgcgagcg atatccggtt caccggcaat cagattgaga
    12661 aaacgcacca tcgccgcttc ggcatcgagc atcccttcat ccatgttgat atcgataatc
    12721 tgcgcgccgt tttccacctg ctgacgcgcc acatccagcg cctcgctgta tttctcttct
    12781 ttgatcaggc gcttgaactt agcagagccg gtgacgttgg tgcgttcacc cacgttcaca
    12841 aacaggctat cgtcgccaat gttcagcggc tccaggccag acaaacggca ggctacggga
    12901 atttccggca gtttgcgcgg cgctaatcct tctactgcac gactcatcgc tgcaatatgt
    12961 tgcggcgtgg tcccgcagca accaccgacg atattgagaa agcccgcttg cgcccattca
    13021 cgtatctgtt ttgccatcgt gtcggcgtcg agatcgtatt caccaaaggc gttaggcagc
    13081 ccggcgttcg ggtgggcggt gacgtagcat tccgcaatcc gtgatagttc ctgcacgtac
    13141 tggcgcagtt catcgggccc cagcgcacag ttcaggccaa aggtcagcgc ttcggcgtgg
    13201 cgcaatgagt tgtaaaatgc ttcggtggtc tgcccggaga gcgtgcgccc ggaggcgtcg
    13261 gtgatggtgc cggagatcat aatcggcagc tcaacgccca gcgcttcaaa ctccgttttc
    13321 accgcaaaga ccgccgcttt ggcgttaagg gtgtcgaaaa cggtttcaat caggatcaga
    13381 tccgcaccac cttccaccag cgctttggtg gattcgcgat aggccgccgc cagctggtca
    13441 aaagtgatgt tgcggaatgc cggatcgttg acgtccggag aaatagacgc cgtgcggttg
    13501 gttgggccaa gaacaccggc aacgtagcgc ggtttctccg gcgtgcgcgc ggtccactcg
    13561 tcagcacagg cgcgcgccag tttcgccgcc gcaaagttga tttccgccga cagggattcc
    13621 atctggtaat ccgccatcgc aatggtcgtg gagttgaagg tgttggtttc gatgatatcc
    13681 gcgcccgctt caaagtaggc gttgtggata gcggcgatca cttccggttt actgagtacc
    13741 agcaggtcgt tgttgccttt gaggtcgcat ggccagtcgg caaagcgttc accacgaaaa
    13801 tcggcttcgt tcagtcgata gctctggatc atggtgccca taccgccgtc caacaccaga
    13861 atacgttcat ttaactgcgc acgcagttgt tccactttgc tgctcacact tgctcccgac
    13921 acgctcaacc cagatttaat aaaaattcaa caaaccatac tggaataaac gcatctgtgg
    13981 taaaagcgac caccacgcaa catgagattt gttcaacatt aactcatcgg atcagttcag
    14041 caactattgc attagctaac aataaaaatg aaaataattt ccacgataca gaaaaaggag
    14101 gctgtcatgg tcgcacccat tcccgcgaaa cgcggcagaa aacccgccgt tgccaccgca
    14161 ccagcgactg gacaggttca gtctttaacg cgtggcctga aattactgga gtggattgcc
    14221 gaatccaatg gcagcgtggc actcacagag ctgtcgcaac aagccgggtt acccaattcc
    14281 acgacccacc gcctgctaac cacgatgcaa cagcagggtt tcgtgcgtca ggtcggcgaa
    14341 ctgggacact gggcaatcgg cgcacatgcc tttatggtcg gcagcagctt tctccagagc
    14401 cgtaatttgt tagcaattgt tcaccctatc ctgcgcaatc taatggaaga gtctggcgaa
    14461 acggtcaata tggcggtgct tgatcaaagc gatcacgaag cgattattat cgaccaggta
    14521 cagtgtaccc acctgatgcg aatgtccgcg cctatcggcg gtaaattgcc gatgcacgct
    14581 tcaggtgcgg gtaaagcctt tttagcccaa ctgagcgaag aacaggtgac gaagctgctg
    14641 caccgcaaag ggttacatgc ctatacccac gcaactctgg tgtctcctgt gcatttaaaa
    14701 gaagatctcg cccaaacgcg caaacggggt tattcatttg acgatgagga acatgcgctg
    14761 gggctacgtt gccttgcagc gtgtattttc gatgagcacc gcgaaccgtt tgccgcaatt
    14821 tctatttccg gaccgatttc acgtattacc gatgatcgcg tgaccgagtt tggcgcgatg
    14881 gtgattaaag cggcgaagga agtgacgctg gcgtacggtg gaatgcgctg actttttctg
    14941 gcgggcagag gcaatattct gcccatcata cctgagtggc aatagaataa gggtgtctgt
    15001 taatcgcatt gacgccaaaa taacttaatg tcatacactt cacacacttt tattaactca
    15061 gcattatttt taaacatcaa accacttaat tattaacaaa at
//
LOCUS       DAJTQY010000089        15050 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.089, whole genome shotgun sequence.
ACCESSION   DAJTQY010000089 DAJTQY010000000
VERSION     DAJTQY010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15050
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.089"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(<1..176)
                     /locus_tag="OO349_003026"
     CDS             complement(<1..176)
                     /locus_tag="OO349_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5868020.1"
                     /translation="MVANINLIKQESYSVVNLEKQLMSNKSPKQTLLNQFSLCLKMEG
                     EEQLCDCVNELKHG"
     gene            complement(592..1533)
                     /gene="fabY"
                     /locus_tag="OO349_003027"
                     /gene_synonym="yiiD"
     CDS             complement(592..1533)
                     /gene="fabY"
                     /locus_tag="OO349_003027"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HCR5868021.1"
                     /translation="MYHLQVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMVVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(1578..2015)
                     /gene="dtd"
                     /locus_tag="OO349_003028"
     CDS             complement(1578..2015)
                     /gene="dtd"
                     /locus_tag="OO349_003028"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HCR5868022.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(2012..2884)
                     /locus_tag="OO349_003029"
     CDS             complement(2012..2884)
                     /locus_tag="OO349_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HCR5868023.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(2878..3477)
                     /gene="yihX"
                     /locus_tag="OO349_003030"
     CDS             complement(2878..3477)
                     /gene="yihX"
                     /locus_tag="OO349_003030"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HCR5868024.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(3576..4361)
                     /locus_tag="OO349_003031"
     CDS             complement(3576..4361)
                     /locus_tag="OO349_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR5868025.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEKKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            complement(4395..5291)
                     /gene="yihV"
                     /locus_tag="OO349_003032"
     CDS             complement(4395..5291)
                     /gene="yihV"
                     /locus_tag="OO349_003032"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HCR5868026.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDAGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDTK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATSGDLAESVRFASGVAALKCT
                     RPGGRAGLPDCDQTRSFLSLFV"
     gene            5459..6355
                     /gene="yihU"
                     /locus_tag="OO349_003033"
     CDS             5459..6355
                     /gene="yihU"
                     /locus_tag="OO349_003033"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HCR5868027.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRNVLFGENGVCEGLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGHGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            6379..7257
                     /gene="yihT"
                     /locus_tag="OO349_003034"
     CDS             6379..7257
                     /gene="yihT"
                     /locus_tag="OO349_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HCR5868028.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKALSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLCASQRLNDHI
                     NMPWVILSSSVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            7274..8515
                     /gene="yihS"
                     /locus_tag="OO349_003035"
     CDS             7274..8515
                     /gene="yihS"
                     /locus_tag="OO349_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HCR5868029.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYALVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTAYALYTVTGD
                     RQYETWYQTWWEYCIKYLMDYENGSWWQELDADNKVITKVWDGKQDIYHLLHCLVIPR
                     IPLAPGMAPAVAAGLLDINAK"
     gene            8653..9555
                     /locus_tag="OO349_003036"
                     /pseudo
     CDS             8653..9555
                     /locus_tag="OO349_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709679.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
     gene            9754..11790
                     /gene="yihQ"
                     /locus_tag="OO349_003037"
     CDS             9754..11790
                     /gene="yihQ"
                     /locus_tag="OO349_003037"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HCR5868030.1"
                     /translation="MDTPRPQLLDFQFHQNNDSFTLHFQQRLILTHNKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDQGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRITSFGKRVMWNWKWNSENYPQLDSRIKQWYQEGVQFLAYI
                     NPYVASDKDLCEEAAQHGYLAKDASGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGVSAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTTVFT
                     TLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAHTYSLKYQYLLGRDILVAPVHEEGRS
                     DWTLYLPEDNWVHAWTGEAFRGGEVTVNAPIGKPPVFYRADSEWAALFASLNSI"
     gene            11836..13210
                     /locus_tag="OO349_003038"
                     /pseudo
     CDS             11836..13210
                     /locus_tag="OO349_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            13253..14656
                     /locus_tag="OO349_003039"
     CDS             13253..14656
                     /locus_tag="OO349_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5868031.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLCIGTLYLLKLYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFCLPVKTTIATTLFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPVTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEKKTASVTL"
     gene            14724..>15050
                     /gene="ompL"
                     /locus_tag="OO349_003040"
     CDS             14724..>15050
                     /gene="ompL"
                     /locus_tag="OO349_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="porin involved in the transport of small molecular
                     weight solutes (up to 600 Daltons) across the cell wall;
                     specific substrate still unknown; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpL"
                     /protein_id="HCR5868032.1"
                     /translation="MKKINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLINDKSISS
                     GGAVYLD"
BASE COUNT         3699 a         3874 c         3744 g         3733 t
ORIGIN      
        1 aaaccatgct tcagttcgtt aacacaatcg cacagttgct cctcaccttc cattttaagg
       61 caaagtgaaa attgatttaa tagcgtttgc ttcggtgatt tattactcat tagttgtttt
      121 tctaaattaa caacagaata actttcttgt ttaatgagat taatattggc aaccattata
      181 gcctccttcc cacggtttaa gagcaaacat acaacataat gattcataac atacagagca
      241 gtaaatgaat gatactgaca atgtatgcca gcccattatt tttgcataat atttgtgagc
      301 tatagatttt aaaaatattt atgaaaaaag tatggcacca gagggggttt tgactatcaa
      361 aaatcgctca aacctgaact aatatgatga gttaagcatt atctgcaata attgccactc
      421 agctctacaa ataatattta aatgctcctg aacagtgatg tagcaagaca cgtatggtgc
      481 caacgaagaa taaaaattta taaagttctt attcaatata accggaatag tcagggactg
      541 atgtaaatga ccggaatgaa tgagagaggc agcaccgccc ctctccataa gctactcttc
      601 ttcgttcccg ccctcttcat acgggccaaa tggcttcgcg ggcagaacga tatacgtgcc
      661 ttcaaacact gcacccggcg tctcgtcgcc aaagatttcg acctgcatct gcacccgtgc
      721 ttttcgtccg cgcgccagac ggtcgagatc gccgcttaag gcaccaaggt cggctaccgc
      781 atggggttta ccgctaatcg gtttgctgta gcggatatgc gcatccgcca gaataatcgt
      841 tccgccgagg tggcgttcac gcagcatcag ccagataagc ccccaaccgg tgagcgtcgc
      901 cagcgagaat aaactcccgg caaacagcgt atggtgcgga ttctgattgc cggtttccgg
      961 catggtggtg ataaattttt gcccggtata ttgttgaata cgcacgccca ttttttcact
     1021 aagcgggata tgttcgtacc acgcctgttg cagctgcgcg caccagtcgc cgcgatgcaa
     1081 aatatcatcc agagtggcga cgggcttaat catcaaaaaa tggcgaatcg gcgtggtggt
     1141 tggcgtggtg atttctccct gattaacaaa ccccagcttg gcgaaaaact ccaccgcgtc
     1201 ttcacgggcg ctacaggtca cgcgcttaac gccttcctga cgcgccaccg actccagggt
     1261 catcgccatc agcgttccta accctttgtc ctgcacgtcc gggtgaacga ccataaagcg
     1321 aatggacgct tcattgtcgg cattaatata caatcggcct accgccacca gattaccctg
     1381 ctcgtcgacg accatctggt gatgcgccat cgcatcccac gcgtcgcgtt ccgaaccttt
     1441 tggttgatgc aggggcttac gcaacatttc ccagcgaaac tgatagtaac gctctaactc
     1501 ttcttctgtt tgtggaacct gaaggtgata catagcgata ctctctcttg tttcccgtga
     1561 caaccctgga agctggctca tacctgcaac cagaatgtca cggggccatc attgaccagc
     1621 gatacctgca tatcggcagc gaagcgtcct gtttgcgtgt tcatctcttg ctggcggcag
     1681 cgttcgacga aatagtcata taacgcctct gcgcgatccg gtgatgcgcc tttggagaaa
     1741 cttgggcgca tcccccgttc ggtatctgcg gcgagggtaa actgggaaac caccagtaca
     1801 ctgccgcccg cctgttgcac gttgagattc atcttgcctt cggcatcgct aaagatgcgg
     1861 tagccgagca cacgctcgca cagacggttt gctttctgtt cgtcgtcatc cttttcgaca
     1921 cccaataaca ccaaaagtcc cgcgccaatt tcgcccgtca cttctccctc cacggtgacg
     1981 ctggcacggg ttacgcgttg aattaatgca atcatggttc gtcgtcttct tcttgttcag
     2041 ctgcttgttt tagtttgcgg tattccccga gagtgacagt aatttccgcg ccaagcaaga
     2101 cgatacacca tgtccagtag acccaaacaa agagaatggg gatcaccgcc agcacaccgt
     2161 aaatgagctg atatgacggg aacatggtga tataaagcgc gaaacctttc tttcctgctt
     2221 cgaacaggag tgcggcgaca aacgcgccga caatcgcgtc gcggttaggt acgcggatgg
     2281 taggaacaat gctgtacagc aaccagaagg agatccacga caacagcaac ggaaaaatac
     2341 gcagcacgtt gtcgatgaca gtattgagat cgctcgccca gcgcagggag agcaaatagg
     2401 aactgatcgc cagactggcc cctgccagca gcggccccag cgttaaaatc atccagtaca
     2461 cggcgaacga gtaaattttt ggtcgcgctc gtttactgcg ccagatggta ttcaacgcgc
     2521 tatcgatgga gtacatcaac aataacgcgg tgacgatcag cccgcacgcc ccaacggcgg
     2581 tcatcttgtt ggaattagca acaaattgtt cgatataccg ctgaataaca tcgccagtag
     2641 caggcagaaa gttggcaaaa ataaagtgac gtaactgaat gctgacgtcg gaaaacatgg
     2701 gaaaagcggc gaaaagcgca aaaacaacgg caaccagcgg cactaatgag agcaacgaca
     2761 cataggcaag gttacctgcc agggttgtca tgttgtcctc atcaatacgt tgccagagta
     2821 gttttagcca ggcccatagt ggacgggtac ggtgcctggc tttgtcctga atggttttta
     2881 gcataacacc ttcgcgaaat agtccgggat ggtggtttta tctttcacca gaatactggt
     2941 aatgcctagc tgattagctc cttctatatt atcggcgtta tcgtcgaaaa agaccgtatc
     3001 gctgggtgaa aaaccttccg cctgcaaaac atgctggtaa attcgtgctt caggtttgcg
     3061 catccccaga tcttgcgaca gatagatatg gtcagcagca tcacgaattt ccgggtattc
     3121 ttccggccag aaggtggtat gcaggcggtt ggtattggaa agcaccacca cgcgatgccc
     3181 ctgctcacgc agtttatgca tgatggcgat cacttccggg cgcagcgcaa caaacaccgc
     3241 ctgccagccg tgagagaact gctcgtagct tagcggtaga gccatctcat gacacagcgc
     3301 ctctgcgaac gcttcatcgc taatttcccc acgctcatgc tgatgaaacg cctcccccat
     3361 atgaaaactc ttcttaagcg atgccagcgg aatacgcgtt aaatcgctcc aggctcccag
     3421 cacacggtta aagtcgatat cgacaatcac attacctaaa tcaaagatat agagcatttt
     3481 tgcctccttt cgcgccacaa gaaattaact gtagcgggaa aggatgggtt tggctatgtg
     3541 gcaaggtatg aagccaggtt aacggtcaca aaaatctacg cgtcttcctg gggaagaatg
     3601 atttcaattt ggctgtcttg taagcgtgat tttagcgcag cgggcggcag ctcgtcggta
     3661 aagagcgcag tgacctgtgc cacgttacca atttcaaccg ccgccgaagc atgatactta
     3721 gtgtgatcgg cgaccagcag aatatttctc gcgtgcgcca tcatcgtttt caccacgtta
     3781 gcttcgttta catcaaactc catcaacgcg ccatcgctct caatcgcccc aacgcttgtt
     3841 accagataat cagcacgaaa atcagccaca aaggacgccg ctgaagggcc aatgatcccg
     3901 ctattatgag agcgcaacgt accgccgggc accatcactt caaagcgcgg gttgtggtaa
     3961 agaatatgcg ccacacgcag gctgttggtg attatccgca aatgattatg gttaagtaac
     4021 gcccgggcaa catgctcaac agtcgtacca atggtgataa atattgttga accatcagga
     4081 atatagtctg ccaccgcttc ggcaatcgct ttttttttct cggtttgcga aacctcacgc
     4141 tgctcgaacg ccgtattaac gacgctggaa gcccgacccg cgccaccgtg atggcgcgta
     4201 atcaggcctt gctcgcttaa tttacgaata tcccggcgga ccgtctgcgt ggagacatcc
     4261 agcagatttg ccagctcatc aatattcata tacccacgct cggcgatcag catgaggagt
     4321 tggtcgtgcc gcgggttacc ggttagttcg gtaaggctca tgaaaattcc tcgaaaaacc
     4381 atcaccctgg cattttatac aaaaagtgac aaaaaagatc gggtttgatc acagtcaggg
     4441 agcccggctc gtccaccggg acgtgtgcat tttaacgccg ctacaccgct ggcgaagcgg
     4501 actgactccg ctaaatcccc acttgttgcc agcgccaccg ccaaagcgcc gtgaaaaaca
     4561 tcacccgcac ctgtggtatc taccacatca actttgaagg ccggttgatg ctgacgccca
     4621 ccattttcca gccagtcgca gcctgcgcta ccctgggtca catagacatg accatttgtg
     4681 agcgtttgtg cctgttttag cgcactggcc atctctttca cgcccgttaa gcgcgccaga
     4741 cccggttctg aaaaggccgc gtgatcgctt aatgccacca gctcactgat atcctgcggc
     4801 gtaatgtccc cgtccagaac ggtcatcaca cccgcctgac gggccagggt gaaggctttt
     4861 ttagcgccgt cgtgccagcg tacatctgcc agcacaacat cccactgaga gaaatcaatt
     4921 tcctccaacc actctgcgtc aggcagcaga tccgggctgg ggtagttaat gattatccgc
     4981 tcgcctttgg tatccaccat gatggcggat tgcgaagatt tcgcctggtt ataccgtttg
     5041 gtgtaacggg tgttaacgcc ccaggattcc agctctgcca gcaggctgtt gcccgcgtcg
     5101 tcatcaccta cgcgaccaat aaaatcgacc tgcgccccca gccttgccgc cgcaaccgct
     5161 gccgtcgccg ctggcccgcc accaacttcc gtataatttc tcgccacgta tttaccgctc
     5221 tccgtcggta acccttccac gtaatagatg cgatccatca cggttatacc tacacaagca
     5281 acacgaatca tggtcattcc ttaaacattt tgggatgcgt tcattttaat ttcaccaatt
     5341 gtttaaaaag tgacggctgt caattttttg acgataaatt acaaattcga tcaaaaacag
     5401 acaagcaaag catgtcataa atgacaaaaa gtgacatcac tgtattcagg agagggttat
     5461 ggcagcaatc gcgtttatcg gtttaggaca aatgggttcg ccaatggcga gcaatttatt
     5521 gcagcaaggg catcaacttc gcgtctttga tgtgaacgcc gaagctgtgc ggcatctggt
     5581 agacaaaggt gcgactcccg ccgccaaccc ggcgcaggcc gctaaagatg ccgaatttat
     5641 cattaccatg ttgccgaatg gcgatctggt gcgcaacgtg ttgttcggtg aaaacggcgt
     5701 ttgcgaaggc ttatctaccg atgcgctggt cattgatatg tccaccatcc atccgctgca
     5761 aaccgataaa ttgattgccg atatgcaagc caaaggcttc agcatgatgg atgttccggt
     5821 aggccgtact tctgcaaatg ccattaccgg tactctgtta ctgctggctg gcggcaccgc
     5881 tgaacaagtt gaacgtgcca caccgatcct gatggcgatg ggcagtgagt tgatcaacgc
     5941 tggcggtccg ggcatgggga tccgcgttaa gctcatcaac aactacatga gcatcgcgct
     6001 caatgcgctt tcggcagaag ccgccgtttt gtgcgaagcc ctgaatcttc ccttcgatgt
     6061 tgccgtcaaa gtgatgagcg gtaccgccgc cggtaaaggt cacttcacca cttcctggcc
     6121 aaacaaagtc ctcagcggag atctttctcc cgccttcatg atcgatcttg cccataagga
     6181 tcttggcatc gcccttgatg tcgccaacca gctgcatgtg ccaatgccgc tgggggccgc
     6241 ctcacgggag gtttatagcc aggcgcgcgc agcgggtcac ggtcgccagg actggtccgc
     6301 cattctggaa caggtccgtg tcagtgccgg gatgactgcc aaagtaaaaa tgtaacgact
     6361 ggataaagga ataaatgaat gaataagtac accatcaacg acattacgcg cgcatcgggc
     6421 ggttttgcca tgctggcggt cgatcagcgc gaagccatgc gcatgatgtt tgccgcggct
     6481 ggggcacccg ctccggtagc cgatagcgtt ttaactgatt tcaaagttaa cgctgcaaag
     6541 gccctctcgc cttatgcctc ggcgattctg gtagatcaac aattctgcta ccgccaggtg
     6601 gttgagcaaa acgcgattgc caaaagttgc gccatgattg tcgccgccga tgagttcatt
     6661 cctggcaacg gtattccggt cgatagcgtg gttattgacc gcaaaatcaa tccgctacag
     6721 atcaaacaag acggtggcaa agccttaaaa ctgctggtgc tgtggcgtag tgatgaagat
     6781 gcgcagcaac gtctggatat ggtgaaagag ttcaacgaac tgtgccactc acacggtctg
     6841 gtaagcatca ttgagccagt cgtccgccca ccgcgtcgtg gcgataaatt cgatcgcgaa
     6901 caagcgatca tcgatgccgc caaagagctg ggcgacagtg gcgctgacct ctacaaagtt
     6961 gaaatgccgc tttatggcaa aggtccgcaa caagagcttc tctgtgcttc acaacgtctg
     7021 aatgaccata tcaatatgcc atgggtgatc ctctcttcca gtgtcgacga aaaactgttc
     7081 ccgcgtgccg tacgcgtggc aatgacggcg ggcgcatcgg gattcctggc aggccgtgca
     7141 gtctgggcat cggtcgtcgg tttaccagac aacgagctga tgctccgtga cgtttgcgca
     7201 ccgaaattac aacaacttgg cgatatcgtc gacgaaatga tggctaaacg ccgctaagat
     7261 gaggataaaa ggaatgaaat ggtttaacac cctaagccac aaccgttggc tggaacagga
     7321 aaccgaccgc atctttgatt ttggtaaaaa ttccgtagtg ccgactggtt ttggctggtt
     7381 aggcaataaa gggcaaatca aagaagagat gggcacccat ctgtggatca cagctcgtat
     7441 gttgcacgtt tattccgttg ctgcggcgat gggtcgacct ggcgcttacg cgttggttga
     7501 tcacggtatt aaagccatga acggcgcact gcgtgataaa aaatatggcg gctggtatgc
     7561 ctgcgtgaat gacgagggcg tggtggatgc ctccaaacag ggctatcaac acttctttgc
     7621 tctgctgggt gccgccagcg ccgtcacaac gggtcacccg gaagcgcgca agctgctcga
     7681 ttacaccatt gagattatcg agaaatattt ctggagcgaa gaagagcaga tgtgcctgga
     7741 gtcctgggac gaagccttca gcaaaaccga agagtaccgc ggcggcaatg ccaatatgca
     7801 cgcggtggaa gctttcttga ttgtttatga cgtcactcac gacaaaaaat ggctggatcg
     7861 cgcgattcgc gtggcttccg tgattatcca cgacgtcgcc agaaataatc attatcgcgt
     7921 taacgaacac ttcgataccc agtggaatcc gctgccggat tacaacaaag ataacccggc
     7981 gcatcgcttc cgcgcgttcg gaggtacacc aggccactgg atcgaatggg gccgtttaat
     8041 gctgcacatc catgctgccc tggaagcccg ttgcgaacaa ccaccagcat ggctgctaga
     8101 agatgccaaa ggtctgttta acgccaccgt gcgcgatgcc tgggcacccg atggagcgga
     8161 cgggattgtt tataccgttg actgggaagg aaaaccggtg gtccgcgaac gtgtacgttg
     8221 gcctatcgtc gaagcaatgg gtacggccta cgcgctctac accgtcactg gcgatcgcca
     8281 gtatgaaacc tggtatcaaa catggtggga gtactgcatt aagtacctga tggactatga
     8341 aaatggttcc tggtggcagg agctggatgc ggacaataag gtcatcacca aagtctggga
     8401 cggcaaacag gatatttatc acctgctgca ttgcctggtg atcccgcgta tcccgttagc
     8461 gcccggcatg gctccagcgg ttgcagcggg tctgctggat attaatgcga aataactgat
     8521 ctgaaaatag ggtgcgggtt ttcccctcac cctaaccctc tccccaaagg ggcgagggga
     8581 ccgtccggtg cagtttttgg gtttgacatc agcctcacgg gaggctgtat gtcgttaatt
     8641 aaggtgcctt gtatgcaaat caccaatatg cattgcagtg gacagaccgt ttcacttgcc
     8701 gcgggggatt atcacgcgac cattgtgacc gtcggtgctg gtctggctga attaaccttt
     8761 cagggttgtc atttagtcat cccgcacaaa cccgaagaga tgccgctggc gcatctgggt
     8821 aaagtgctta ttccgtggcc gaaccggatc gccaacggct gttaccgtta tcaggggcag
     8881 gaatatcagt tacccattaa tgaacatagt tcaaaagccg ctattcacgg gctgcttgcc
     8941 tggcgagact ggcaaattag tgaattaacc accaccagcg ttaccctgac agcttttctg
     9001 ccgcccagtt acggctatcc gtttatgctg gcttcacaag ttgtttattc gctcaacgca
     9061 cacacgggtc tttccgttga gatcgcaagc cagaatatcg gcactgtcgc agcgccttat
     9121 ggcgtgggta ttcatcctta tttaacctgc aacttaacat ctgttgatga atatctgttc
     9181 cagcttccgg caaactaggt ttatgccgtc gatgaacacg taaatccgac aacgcttcac
     9241 cacgttgatg agctggattt aaatttcacg caggcgaaaa aaatagccgc gacaaaaatc
     9301 gaccacacct ttaaaaccgc taatgatttg tgggaaatga ccattaccca tccacagcag
     9361 gcgctttcgg taagcctttg ttccgatcaa ccctgggtgc aggtttatag cggtgaaaaa
     9421 ttacagcgtc agggactggc agttgaacct atgagttgcc cgcctaacgc ctttaattca
     9481 ggtatcgact tattattact ggaaccagga aaaccgcacc gcctgttttt taatatttac
     9541 ggacagagaa aataacttgt attgattagc agtcaaaatc attgaagaac atttcactcg
     9601 ctaaaggcaa cattatgtat cttcaattta ttaagttaaa aacagctcag cttctgtaaa
     9661 gtttttatat atcagtttta tgagaatcat tttacataag cctgtaataa caggaaggca
     9721 acaatctcgt tctcaactct atggagatta gttatggata cgccacgtcc acagttatta
     9781 gattttcaat ttcatcagaa taacgacagt tttaccctac attttcaaca acgtcttatt
     9841 ttaacccata acaaagataa tccttgttta tggattggct caggtatagc ggatatcgat
     9901 atgttccgcg gtaatttcag cattaaagat aaactacagg agaaaattgc gcttaccgac
     9961 gccatcgtca gccagtcacc ggatggttgg ttaattcatt tcagccgtgg ttctgacatt
    10021 agcgccacgc tgaatatctc tgccgacgat cagggccgtt tattgctgga actacaaaac
    10081 gacaacctta accacaaccg tatctggctg cgccttgccg ctcaaccaga ggaccatatc
    10141 tacggctgcg gcgaacagtt ttcctacttc gatctgcgcg gcaaaccgtt cccgctatgg
    10201 accagtgaac aaggcgttgg tcgcaacaaa caaacctatg tcacctggca ggccgactgc
    10261 aaagaaaatg cgggcggcga ctattactgg actttcttcc cacagcctac gtttgtcagc
    10321 acgcagaagt attactgcca tgttgataac agttgctaca tgaacttcga ctttagcgcc
    10381 ccggaatacc atgaactggc gctgtgggaa gacaaagcta cgctacgttt tgaatgtgct
    10441 gacacataca tctccctgct ggaaaaatta actgccctgc tgggacgcca gccagaactg
    10501 cccgactgga tttatgacgg ggtaacgctc ggcattcagg gcgggacgga agtgtgccag
    10561 aagaaactgg acaccatgcg taacgcgggc gtgaaggtta acggcatctg ggcgcaggac
    10621 tggtctggga tccgtataac ctcttttggc aagcgcgtga tgtggaactg gaagtggaac
    10681 agcgaaaact acccgcaact ggattcacgc attaagcagt ggtatcagga gggcgtgcag
    10741 ttcctggcct atatcaaccc gtatgttgcc agcgataaag atctctgcga agaagcggca
    10801 caacacggct atctagcaaa agatgcctct ggcggtgact atctggtgga gtttggcgag
    10861 ttttacggcg gcgttgtcga tctcactaat ccagaagcct acgcctggtt caaggaagtg
    10921 atcaaaaaga acatgattga actcggctgc ggcggctgga tggctgactt cggcgagtat
    10981 ctgcccaccg acacgtacct gcataacggc gtcagtgccg aaattatgca taacgcctgg
    11041 cctgcgctgt gggcgaagtg taactacgaa gcccttgaag aaacgggcaa gctcggcgag
    11101 atcctcttct ttatgcgcgc cggttctacc ggtagccaga aatactccac catgatgtgg
    11161 gcgggcgacc agaacgtgga ctggagtctc gacgatggcc tggcgtccgt agtcccggcg
    11221 gcgctgtcgc tggcaatgac cggacatggt ctgcatcaca gcgacattgg cggttacacc
    11281 accctgtttg agatgaagcg cagcaaagag ctgctgctgc gctggtgcga tttcagcgcc
    11341 ttcacgccga tgatgcgcac ccacgaaggt aaccgtcctg gcgacaactg gcagtttgac
    11401 ggcgatgcgg aaactatcgc ccattttgcc cgtatgacta ccgttttcac caccctgaaa
    11461 ccttatctga aagaggccgt cgcgctgaat gcgaagtccg gtctgccggt catgcgcccg
    11521 ctgttcctgc attacgaaga cgatgcacat acctattccc tgaaatatca gtacctgtta
    11581 ggccgcgaca ttctggtcgc tccggtacac gaagaaggtc gcagcgactg gacgctctat
    11641 ctgccggagg ataactgggt acatgcctgg acgggtgaag cgttccgggg cggggaagtt
    11701 accgttaatg cgcccatcgg caagccgccg gtcttttatc gcgccgatag cgaatgggcg
    11761 gcactgttcg cgtcgttaaa cagcatctaa tcacatccgc ccgtggggaa actcatgggc
    11821 aaagggagaa catcgatgag tcacatcaca acggaagatc cagcaacttt acgcctgccc
    11881 tttaaagaga aactctctta cggtattggc gacctggcct ctaacatcct gctggatatc
    11941 ggtacgcttt atcttttgaa gttttatacc gacgttctgg ggctgccagg cacctatggc
    12001 ggcattatct ttttgatttc aaaattcttt actgcgttta ccgatatggg taccggcatt
    12061 atgttggatt cccgacgcaa gatcggtcca aaaggtaagt tccgtccttt tattctgtat
    12121 gcgtcattcc cggtcacctt actggcgatc gccaactttg tcggcacacc gtttgatgtc
    12181 accggtaaaa cggtgatggc cactattctg tttatgcttt acggactgtt tttcagcatg
    12241 atgaactgct cctacggcgc aatggtcccg gcaatcacca aaaaccccaa cgaacgcgcc
    12301 tcactggcgg catggcgtca gggcggcgct acgctgggcc tgctgctgtg cacggtggga
    12361 ttcgtgccag ttatgaatct tatcgaaggt aatcagaaac ttggctatat cttcgccgcc
    12421 acgctgtttt cactgttcgg cctgctgttt atgtggatct gctactcagg cgtgaaagag
    12481 cgttatgtcg aaacccaacc aaccaatccg gcgcaaaagc ctggcctgtt gcaatctttc
    12541 cgcgcaattg ccggtaaccg cccactgttc attctgtgta ttgccaacct ctgcacctta
    12601 ggggcgttta acgtcaagct cgccattcag gtctattaca cccagtacgt acttaacgat
    12661 cccatcctgt tgtcgtatat gggatttttc agcatgggct gtattttcat cggcgtgttc
    12721 ctgatgcctg gcgcagtcag acgttttggt aagaagaagg tctatatcgg cggcctgctg
    12781 atttgggtgc tgggcgatct gctcaactat ttcttcggcg gcggctcggt cagctttgtg
    12841 gcgttctcct gcctggcgtt cttcggctca gcgcctgaac tgggcgctgg tttccgacac
    12901 cgtcgagtac ggcgagtggc gcaccggcgt gcgttcggaa ggcacggtct acaccggctt
    12961 caccttcttt cgcaaagtgt ctcaggcgct ggctggtttc ttccccggct ggatgctgac
    13021 gcaaattggc tatgtgccga acgtcgcaca ggctgaccac accattgaag ggttacgcca
    13081 gttgatcttc atctacccaa gcgcactggc ggtagtcacc attgtggcga tgggttgctt
    13141 ctacagcctg aacgagaaga tgtatgtccg cattgtggaa gagatagaag cccgtaaacg
    13201 cacggcgtaa taataataaa cgacgccctg cggggcgtta taaggagtga ttatgtctga
    13261 ccataatcca ctgacattaa aactgaatct gcgggaaaaa atcgcctatg gtatgggcga
    13321 cgtcggttcg aatttaatgc tctgcatcgg tactctgtat ctcctcaaac tttataccga
    13381 tgaattaggg atgcctgctt actatggcgg gattatcttt ctggtcgcga agttttttac
    13441 cgcgtttacc gatatgctca ccggattttt actcgactcg cggaaaaata ttgggcctaa
    13501 aggcaaattt cgtcccttta ttttatatgc agcagttccg gcggcgttaa ttgcaacgct
    13561 ccagtttatc gccaccacct tttgtttgcc ggttaaaacg acgattgcca ccacgctgtt
    13621 tatgatgttt ggactttcat acagtctgat gaactgctcg tatggtgcga tgatcccggc
    13681 aattaccaaa aacccgaatg agcgcgcgca gctcgcggct taccgccagg gtggtgcaac
    13741 catagggcta ttgatttgta ccgtggcgtt tattccgttg cagtcgcttt tttctgactc
    13801 aaccgtcggt tatgcctgtg cggcacttat gttctccatt ggcggcttta tttttatgat
    13861 gctgtgctac agaggcgtca aagagcatta tgtggacaca gcgccaaccg gacataaagc
    13921 cagtattctc aaatcttttt gcgcgatatt tcgtaatcca ccattgctgg ttttatgcat
    13981 tgctaacctg tgtactctgg cggcatttaa tatcaaactg gcgattcagg tctattacac
    14041 ccagtatgtg ctgaatgata ttaatttatt gtcgtggatg ggattcttca gtatgggatg
    14101 catcctcgtc ggcgtattac tggtgccagt aactgtaaaa tgttttggta aaaaacaggt
    14161 ttatttagct ggcatggtgc tatgggcggt gggtgatata ctgaattatt tctggggaag
    14221 taactctttc actttcgtca tgttctcttg tgtcgccttt tttggcacgg cctttgtaaa
    14281 cagcctgaac tgggcactgg taccagatac cgtagattac ggtgaatgga aaaccggtat
    14341 tcgtgccgaa ggttctgttt ataccggtta taccttcttt cgtaaaattt ctgccgcact
    14401 tgctggcttc ttgccaggca ttatgctgac gcaaattggt tatgttccca acatcgcgca
    14461 aagcgatgcg acattacagg gtttgcgtca gctcattttt atctggcctt gtgcactggc
    14521 aattattgct gcattgacga tgggattctt ttacacactc aatgaaaaac ggtttgcatt
    14581 gattattgag gaaatcaacc aacgcaaaaa taaagagatg gcgacagaaa aaaaaacggc
    14641 ttccgtaacg ttataaatgt caatagccgc tatttccatc ctggtgggtg gcggcctccc
    14701 tacgtttaaa aaatggactt attatgaaaa agattaatgc aataatttta ttatcttctc
    14761 tgacctctgc ctcggtattt gctggcgcat acgtcgaaaa cagggaggca tataatcttg
    14821 cctccgatca gggcgaggtt atgctacgtg tgggttataa cttcgatatg ggcgcgggta
    14881 ttatgttaac caatacctac acctttcagc gagaagatga actaaaacat ggctataatg
    14941 aaattgaagg ttggtatcct ttattcaaac caaccgataa attaaccatc cagcccggtg
    15001 gcttaattaa tgataagagt atcagttcgg gtggtgcagt gtatctggac
//
LOCUS       DAJTQY010000090        15032 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.090, whole genome shotgun sequence.
ACCESSION   DAJTQY010000090 DAJTQY010000000
VERSION     DAJTQY010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15032
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.090"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            <1..238
                     /locus_tag="OO349_003041"
     CDS             <1..238
                     /locus_tag="OO349_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HCR5868033.1"
                     /translation="LLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMP
                     ALSEKIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            248..886
                     /locus_tag="OO349_003042"
     CDS             248..886
                     /locus_tag="OO349_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HCR5868034.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCSDSSDVDNDGDGTFYATVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRFGSGKKESYSSGGFTTRKASTVSRGGYGRSSSARGHWGG"
     gene            889..2052
                     /locus_tag="OO349_003043"
     CDS             889..2052
                     /locus_tag="OO349_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCR5868035.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            2136..3761
                     /locus_tag="OO349_003044"
     CDS             2136..3761
                     /locus_tag="OO349_003044"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HCR5868036.1"
                     /translation="MHWQTHTIFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTAGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCV"
     gene            complement(3878..4153)
                     /gene="yjfN"
                     /locus_tag="OO349_003045"
     CDS             complement(3878..4153)
                     /gene="yjfN"
                     /locus_tag="OO349_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HCR5868037.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(4302..4631)
                     /gene="bsmA"
                     /locus_tag="OO349_003046"
     CDS             complement(4302..4631)
                     /gene="bsmA"
                     /locus_tag="OO349_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HCR5868038.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            4813..5562
                     /gene="yjfP"
                     /locus_tag="OO349_003047"
     CDS             4813..5562
                     /gene="yjfP"
                     /locus_tag="OO349_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR5868039.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNKIVAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            complement(5559..6314)
                     /gene="ulaR"
                     /locus_tag="OO349_003048"
     CDS             complement(5559..6314)
                     /gene="ulaR"
                     /locus_tag="OO349_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HCR5868040.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLG
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(6422..7486)
                     /gene="ulaG"
                     /locus_tag="OO349_003049"
     CDS             complement(6422..7486)
                     /gene="ulaG"
                     /locus_tag="OO349_003049"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HCR5868041.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEIKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            7841..9238
                     /gene="ulaA"
                     /locus_tag="OO349_003050"
     CDS             7841..9238
                     /gene="ulaA"
                     /locus_tag="OO349_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HCR5868042.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASLIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVHWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWVVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            9254..9559
                     /gene="ulaB"
                     /locus_tag="OO349_003051"
     CDS             9254..9559
                     /gene="ulaB"
                     /locus_tag="OO349_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HCR5868043.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            9569..10033
                     /gene="ulaC"
                     /locus_tag="OO349_003052"
     CDS             9569..10033
                     /gene="ulaC"
                     /locus_tag="OO349_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HCR5868044.1"
                     /translation="MKLHDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            10047..10697
                     /gene="ulaD"
                     /locus_tag="OO349_003053"
     CDS             10047..10697
                     /gene="ulaD"
                     /locus_tag="OO349_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HCR5868045.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            10707..11543
                     /gene="ulaE"
                     /locus_tag="OO349_003054"
     CDS             10707..11543
                     /gene="ulaE"
                     /locus_tag="OO349_003054"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HCR5868046.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETN
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKARMAKAGMVE
                     AA"
     gene            11543..12229
                     /gene="ulaF"
                     /locus_tag="OO349_003055"
     CDS             11543..12229
                     /gene="ulaF"
                     /locus_tag="OO349_003055"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HCR5868047.1"
                     /translation="MQKLKQQVFEANMDLPRYVLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     EIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSYLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(12358..12633)
                     /locus_tag="OO349_003056"
     CDS             complement(12358..12633)
                     /locus_tag="OO349_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR5868048.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            12960..13355
                     /gene="rpsF"
                     /locus_tag="OO349_003057"
     CDS             12960..13355
                     /gene="rpsF"
                     /locus_tag="OO349_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HCR5868049.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            13362..13676
                     /gene="priB"
                     /locus_tag="OO349_003058"
     CDS             13362..13676
                     /gene="priB"
                     /locus_tag="OO349_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HCR5868050.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            13681..13908
                     /gene="rpsR"
                     /locus_tag="OO349_003059"
     CDS             13681..13908
                     /gene="rpsR"
                     /locus_tag="OO349_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HCR5868051.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            13950..14399
                     /gene="rplI"
                     /locus_tag="OO349_003060"
     CDS             13950..14399
                     /gene="rplI"
                     /locus_tag="OO349_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HCR5868052.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(14470..>15032)
                     /locus_tag="OO349_003061"
     CDS             complement(14470..>15032)
                     /locus_tag="OO349_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HCR5868053.1"
                     /translation="TMNGDNSFGWNHEATLDNIHAQKYNKAYAGARLTAELKYLLQDV
                     ESFEPNSKYTIHEVVLGPGYSTPDYTGQTIGYVVTLPAQMPNCWSSELPTIDLYIDQL
                     HTVTGVSNALGFIIAALLNAYSDLPHDLKIGLRSLSNSAAIYSGLGFERVPQDRDIRS
                     DRMHLTPANHPDLWTQENGEWIYLRN"
BASE COUNT         3512 a         3665 c         4050 g         3805 t
ORIGIN      
        1 attgttaggt tacgttatcc cattatccag tgcggcaatc aatgcggtga gtattccaga
       61 ctatttcgcc tggggcggga ttgcactggt gattcagtta ctcgtttttg ctggtgtcag
      121 actttatatg cccgcattaa gcgaaaaaat tattaatcac aataccgcag caggaatgtt
      181 tatgggaacc gccgcgctgg ctggcggtat ttttaacgca gcttgtatga catggtaatg
      241 gacgatcatg gccagaaaac gcaaatcaag aaataacagt aaaattggtc acggagcgat
      301 cagtcgcatt ggtagaccga ataatccttt tgaaccgcgt cgcaatcgct acgcacaaaa
      361 atacttaacg ttggcgctaa tgggcggtgc cgcttttttc gtattgaaag gttgtagcga
      421 tagtagcgat gtcgataacg acggcgacgg aacattttac gcgacagtgc aggattgtat
      481 tgatgacggt aataatgccg acatttgcgc gcgtggctgg aacaacgcca aagcggcatt
      541 ttatgccgat gttccaaaga atatgactca gcagaactgt cagtctaagt acgaaaattg
      601 ctactacgac aatgttgaac agagttggat cccggtcgtt tctggatttt tgttaagccg
      661 ggttattcgt aaagatcgcg atgagccgtt cgtctataac agcggcggat cctcatttgc
      721 ttcccgcccc gtctggcgca acacttccgg tgattactcc tggcgcttcg gttctggcaa
      781 aaaagagtct tactcttcag gcggctttac caccagaaaa gcgtcgaccg tttcgcgcgg
      841 cggctatggt cgttcttcca gcgcccgtgg gcattgggga ggctaatcat gctgagacac
      901 aacgttcctg tgcggcggga tctggaccag atcgccgcta acaacggttt cgactttcat
      961 atcatcgaca atgaaatcta ttgggacgag agtcgggctt accgttttac tctgcgccag
     1021 attgaagagc agatcgaaaa accgactgcg gaactgcatc agatgtgcct tgaggtggtg
     1081 gatcgcgcgg ttaaagacga agagatcctg acgcaactgg cgatcccgcc gttgtactgg
     1141 gatgtgatcg ccgaaagctg gcgcgcacgc gatccttcgc tgtatggccg gatggatttt
     1201 gcctggtgtg gcaatgcgcc ggtgaagctg ctggagtaca acgccgatac gccgacctcg
     1261 ttgtatgagt cggcttattt ccagtggttg tggctggagg atgcccggcg cagcggcatt
     1321 attccgcgtg atgccgatca gtacaacgcc attcaggaac gcctgatttc gcgctttagt
     1381 gagctttaca gtcgggaacc gttttatttt tgctgctgtc aggacaccga tgaagacagg
     1441 agtaccgtgc tgtacttgca ggactgcgcc cagcaggcag ggcaggagtc gcggtttatc
     1501 tacattgaag atctcggttt gggcgtcggc ggcgtactga ccgatcttga tgataatgtc
     1561 atccagcgtg catttaagct gtatccgctg gagtggatga tgcgtgacga taacggtccg
     1621 ctgctgcgca agcgtcgcga gcaatgggtg gagccgttat ggaaaagtat cttgagtaat
     1681 aaagggctaa tgccgctgct ttggcgcttc ttccctggtc atcctaacct tcttgcatcc
     1741 tggtttgagg gtgaaaaatc gcagattgcc gctggcgaaa gctatgtgcg taaaccgatc
     1801 tactcgcgcg aaggcggaaa cgtcaccatt ttcgacggtc agaataacgt cgttgaccac
     1861 gctgatggtg attacgccga tgaaccgatg atctaccagg cgtttcaacc tctgccgcga
     1921 tttggcgata gctacacact catcggtagc tggattgtcg atgatgaagc gtgcggaatg
     1981 gggatccgtg aagataacac actgatcacc aaagacacct cacgtttcgt tccgcattac
     2041 attgctggat aagaatgttt tagcaatctc tttctgtcat gaatccatgg cagtgaccat
     2101 actaatggtg actgccattg atggagggag acacagtgca ctggcaaact cacaccattt
     2161 ttaatcaacc tataccatta aataacagca acttatacct gtctgatggc gcgctttgcg
     2221 aagcggtaac gcgtgaaggt gctggctggg atagcgattt tctagccagt attggtcagc
     2281 agttaggaac ggctgaatcc cttgaactgg ggcggctggc gaatgtaaac ccgcctgaac
     2341 tgttgcgcta cgatgcccag gggcggcgtc tggacgatgt tcgtttccat cccgcatggc
     2401 acctgctgat gcaggcgtta tgtaccaatc gggtgcacaa tcttgcctgg gaagaagacg
     2461 ctcgctccgg cgcatttgtg gcgcgcgcgg cgcgttttat gttacatgcg caggttgagg
     2521 ctgggtcgtt atgtccaata acgatgacct ttgccgccac gccattgttg ttacagatgt
     2581 tacccacgcc gtttcaggac tggaccacgc cgctgctgag cgatcgctac gattctcact
     2641 tattgccagg tgggcaaaaa cgcggtttgt tgattggcat gggaatgacg gaaaagcagg
     2701 gcggttccga tgtcatgagc aacaccaccc gtgcagagcg tttggaagat ggctcttatc
     2761 ggctggtggg gcataaatgg tttttctcgg tgccgcaaag cgatgcgcat ctggtgctgg
     2821 cgcagaccgc gggcggtctg tcctgctttt ttgtgccgcg ctttttgcct gacgggcaac
     2881 gcaacgcgat tcgcctcgag cggctgaaag ataagctggg taatcgctct aacgccagtt
     2941 gcgaagtgga gtttcaggat gccattggct ggttattggg gcaggaaggg gaaggaattc
     3001 gtctgatcct gaaaatgggg gggatgacgc gttttgattg cgccctgggt agccatgcca
     3061 tgatgcgccg tgcattttcg ctggcgattt atcatgcaca tcaacgccat gtttttggta
     3121 atccattgat ccaacagccc cttatgcgtc atgtcttaag tcgcatggca cttcagcttg
     3181 aagggcaaac ggcgttgctg tttcgtcttg cgcgagcgtg ggaccggcgt gccgatgcca
     3241 aagaagccct gtgggcgcgt ttatttacgc ctgcggcaaa atttgtgatc tgcaaacgag
     3301 gtatgccgtt tgtggccgaa gcgatggagg tgctgggcgg cattggttat tgcgaggaga
     3361 gcgagctgcc gcggctttac cgggagatgc cggtaaacag tatttgggaa ggttccggca
     3421 atattatgtg cctggatgtg ttgcgcgttc tcaataagca agcgggcgta tacgacttat
     3481 tgtcggaagc atttgtggaa gtgaaagggc aggatcgcta ttttgatcgc gcggttcgtc
     3541 gtttacagca gcagctgcgt aagccagctg aagaactggg gcgagagatt actcatcagc
     3601 tattcctgct gggctgcggt gcgcaaatgt tgaaatatgc ttctccgcca atggcgcagg
     3661 cgtggtgtca ggtgatgtta gatacgcgcg gcggcgtacg gttgtcagag cagatccaga
     3721 atgatttatt gctgcgggcg acggggggag tgtgtgtgta agcgtatacg actgatgcga
     3781 cgctggtttc gattaactaa atgaaatatg tgaaaattgt aggccggaca aggcgctcgc
     3841 gccgcatccg gcattgttca tttgctgtag atgacactca tgcatacagt atcgcctgta
     3901 cgcgccagtt atcaggctgc tggtcttcgt acatggtaat aatgcggtac catgacgcac
     3961 cttgttcatc ggcctttaac gccacgatac gttccacgtc ctgcggatcc cctgaaatat
     4021 tgctaactga gatctgacct atttcattca ggcccgtcac gcagtcagca ctggcgaatt
     4081 ctgcagactg tgccgtggcg ctcgcaggat aagtcactaa ttgcagcgag ggtgaggcaa
     4141 gtaattgttt catcgtcagc tccatttttc gtttccccac atgttgcagg gcattccatc
     4201 gcggtaataa acgcccaagc ttccagccgg taatttattg tgcacaggta aacgcatagg
     4261 gacgcaaagc aatgtaaagc tgactatgta agttgtctga tttatttacg gtacaaaatg
     4321 gcttgtgaat accactgtcc tgtcacgatg gtttcgtcca ccatgacgac aacgtaataa
     4381 tcagcttttg cagcgacagc ttttgctctg atttctgcta atgcgtcatc cggagaaccc
     4441 cgaaccatcg tgcttacgct acctattcgc tgtaaccctt gcgtctggtc gcggcgaatc
     4501 tcttgcggat ggtccgttac tggcggtgct ggctgtggcg taccttgcag tgcgctacag
     4561 gcacttaaca tcaacaccaa taataaactg gcaaaccggt aaataacgct attacgtttc
     4621 ctgctaacca tagtgtagtg ccttattaat ttaactttgg gggaatgttc ccgaattgtt
     4681 acctggtacg cgattttcga atgaaaacgt gacgaaagcg taacccacat ctcaacatta
     4741 tgaactaata ccgccagcag aaacttgtgc taaggatttc ttcatgtcat accagacaaa
     4801 aaggagagac agatgattga aatagaatca cgcgagctgg cagatattcc cgttcttcat
     4861 gcttatcctg tcgggcaaaa agataccccg ttaccgtgcg taatttttta tcacggcttt
     4921 acttcatcca gcctggtgta tagctatttt gccgttgcgc tggcgcaggc tggtttgcgg
     4981 gtgatcatgc cggatgcgcc cgatcacggt agccgtttta gtggcgacga agcgcggcgg
     5041 ttaaatcaat tctggcaaat cttgctacaa agtatgcagg aattcactac tttacgtgcg
     5101 gcaatagccg aagaaaactg gctgcttgat gaccgtctgg cagtcggtgg cgcgtcgatg
     5161 ggcgcgatga cggcactggg aattaccgct cgccacccca cggtgaaatg taccgccagc
     5221 atgatgggat cgggctattt tacatcgctc gcccgttcac tgtttccacc gctgatacct
     5281 gaaacggcag cacagcagaa tgaattcaat aaaattgtcg cgccgctggc agagtgggaa
     5341 gcgacaaacc acctggaaca acttggggac agacctctac tgctgtggca tggcctcgac
     5401 gatgacgttg tgcctgccga cgaatcacta cgtttgcagc aggccttaag cgagacggga
     5461 cgggataaac tgctaacctg ttcatggcag ccaggcgtgc gtcaccgtat tacgcctgag
     5521 gcgttagatg ctgccgtgac atttttccgc cagcatcttt aaacacgcag aatgctgacc
     5581 ccttgcgctt ccagttgttg caggatttcc gggttagcat ttttgccggt gatcagcata
     5641 tcgatttgat cggcacggct aaaaagcatt cccgcgcgtt cgccaatctt actgctatca
     5701 accagtacca ccagtttccc taccacgctc agcatcttct gctctgccat tgctgtcagc
     5761 atatcggttt tatacaaccc ttctgcggtc agcccttttc cgctggtaaa catccagtgc
     5821 ccggcataga gactgttttc gctgccctgc gggcttaaag tgatggactg acttttgttg
     5881 tactgtccac ccataatgat cacgctgtca tgttcttgat cgatcaggta atttgccagc
     5941 ggtagataat tagtgatgat ttgcactggc ttgccacaca tttcccgccc aagcagaaac
     6001 gcggtggagc cgcagttgat gactacgctt tcgcccggat taaccagctg cgaggccgct
     6061 ttagcaatac gtactttttc atcgtgattc tgcgcctgat gcagattcat cggcgtccag
     6121 cgcgggcgct gttgggtaat agcttctgcg ccattgcgca cctttttcag tttgccgctt
     6181 tcgccaagtt tattgatatc gcgtcgcgca gtggcaggcg aaattcccag acgctcaacg
     6241 actttctcaa cggtcacaaa gcccaattgt gcgagcattt ccaggaggat ttgatgtctt
     6301 tgtgcttcag tcatgagcta ttccgataaa atttgatttt tttagatgat atttgaaata
     6361 gccaggaaat actacgccgg atagcgcgaa cttctccacg ctatccggta taaacgacag
     6421 attacaggaa tgacttgaac ggcagatccg gttcaatagt gaagcaatca tcgaaaccgc
     6481 gcggatagtg gtactcgaag ttgtctttat ccagcggcca ggtaaatttg ccgccaacct
     6541 gccagataaa cggcttgaag ccatacttca gacgatcttt tttgatctcc cacagcacgc
     6601 ggatctcttg cggatcggcc tggaagtttg accagatgtc gtggtggaac gggataacca
     6661 ctttcgcatt cagggcttca cccatacgca gcatatcggc gctggtcatt ttgtcggtaa
     6721 taccgcgcgg gttctcacca tacgatccta acgccacatc gatctgatgt tcgttaccgt
     6781 gcttcgcata atagttagag tagtgggaat cgccactgtg atatagggaa ccgccagggg
     6841 ttttgaacag gtagtttact gcgcgatcgt ccatgccatc tggcagtacg ccagccgctt
     6901 tttgatcggc aggcagggtg atcagtgcag tacggtcgaa agcgtcaagc gcatgaattt
     6961 caatgtcttt cacttttaca acatcgcccg gtttgaccac gatgcaacgc tctttcggta
     7021 cgccccagcc aatccacaaa tcaacgcagg ttttcggtcc aatgaatggt acgtcatcag
     7081 cacagttctg catcacggca gcagcgacgt taacgtcgat atgatcgttg tgatcgtgag
     7141 tcgccagtac cgcgtcgatc tggcgaatcg caaacggatc aagaacaaac ggggtggtgc
     7201 gtaggtttgg ctgcagtttt ttcacgccag ccatgcgctg catctggtga ccctgtttca
     7261 ttaacgggtt accgtgactt tgtttgccag tgccgcacca gaaatcaacg caaacgttgg
     7321 tgccaccttc tgatttcaac cagatcccgg tgcagcctag ccaccacatc gcaaatgtgc
     7381 caggggcgac ctgctcttgt tcaatttctt cattcagcca gctaccccac tccgggaaag
     7441 tgctcaggat ccaggattct cgggtgatac ttttcacttt actcatcgcc atttaccttc
     7501 atgatagttc aattcgaatc aacatgtgat tggttttgat taatcctgac actattcctt
     7561 caggaaggca atgaccattt tttgactttt tgccagggaa gttgttgttg attttgagtg
     7621 tggaaagatt taatggaatg tgtagttaaa ttaactaaat gaatttaaat aaataattgt
     7681 ttcgttgtgt gaatcacgct ctatccgtgt ggaattattt gcgggtcgcg ccacatttaa
     7741 tcataaataa tcttgttgtg attacttttg aaaattagag tgagtacaca acattccggg
     7801 tgtgcggaat acccggttac ctcttcttca ggagatcgtt atggagatcc tctacaacat
     7861 ctttaccgtg ttttttaacc aggtcatgac caatgccccg ttgttgctgg gtattgtgac
     7921 ctgtctgggc tacatcctac tgcgcaaaag tgtcagcgtt attattaaag gcacgattaa
     7981 aaccataatt ggtttcatgt tgttgcaggc agggtccggc atcctcacca gcaccttcaa
     8041 accggtggtg gcgaaaatgt ccgaagtcta cggcattaac ggcgcaattt ccgatactta
     8101 cgcgtcgatg atggcaacca tcgaccgcat gggcgatgcc tatagctggg taggttacgc
     8161 ggtgttgtta gcgctggcgc tgaacatctg ttacgtgctg ctgcgtcgca ttaccggtat
     8221 tcgtaccatc atgctgactg gtcatatcat gttccagcag gcggggctga ttgccgttac
     8281 gttgtttatt ttcggctact ccatgtggac aaccattatc tgcacggcga ttctggtttc
     8341 gctctactgg ggcattacct ccaacatgat gtacaagccg actcaggaag tgacggacgg
     8401 ctgcggtttc tccatcggtc accagcaaca gtttgcatca ttaattgcct ataaagttgc
     8461 gccgttcctc ggcaaaaaag aggaaagcgt tgaagacctc aaactgccag gctggctgaa
     8521 tattttccac gacaacatcg tctccacggc gattgtcatg accatcttct ttggtgccat
     8581 tctgctctcc ttcggtatcg acaccgtgca ggcgatggca ggcaaagtgc actggacggt
     8641 ctatatcctg caaaccggtt tttccttcgc ggtggcgatc ttcatcatca ctcagggtgt
     8701 gcgcatgttt gtggcggaac tctctgaagc gtttaacggt atctcccagc gcctgatccc
     8761 tggtgcggtt ctggcgattg actgtgcggc tatttatagc ttcgctccga acgccgtggt
     8821 ctggggcttt atgtggggca ccatcggtca gctgattgcg gttggcatcc tggtcgcctg
     8881 cggttcctcg atcctgatta tcccaggctt tatcccgatg ttcttctcca acgccaccat
     8941 cggcgtgttc gctaaccact tcggcggctg gcgtgcggcg ctgaagattt gtctggtgat
     9001 ggggatgatc gaaatctttg gttgcgtctg ggtggtgaaa cttaccggta tgagtgcctg
     9061 gatggggatg gcggactggt cgattctggc accgccgatg atgcagggct tcttctccat
     9121 cggtatcgcc tttatggccg tcatcattgt aattgcactg gcttatatgt tcttcgctgg
     9181 ccgcgcactg cgcgcagaag aagatgcaga aaaacaattg gcagaacagt ctgcttaata
     9241 aggagttttg attatgaccg tacgtattct ggctgtgtgt ggcaacggac aaggcagttc
     9301 catgatcatg aagatgaaag tggaccagtt tttaacccaa tcaaacattg accacacggt
     9361 aaatagctgc gcggttggcg aatacaaaag cgagttgagc ggcgcggata tcatcatcgc
     9421 ttctacgcac attgcgggcg aaatcaccgt gaccggcaac aaatacgtgg ttggcgtgcg
     9481 caacatgctc tctcctgccg actttggccc gaaactgctg gaagtgatca aagcgcattt
     9541 cccgcaggat gtgaagtaag gacgcgccat gaaattacat gattcgctgg cggagaataa
     9601 atccatccgc ctgcaggctg aagctgagac atggcaggac gcagtaaaaa tcggcgttga
     9661 cctgctggtg gcggcggatg ttgtcgagcc gcgttactac caggcgattc tggatgcggt
     9721 tgaacagcac ggtccgtact ttgtgctcgc accaggcctg gcaatgccgc acggtcgccc
     9781 ggaagagggc gttaagaaaa ccggttttgc gctggtgacg ctgaaaaagc cgctggagtt
     9841 caaccacgaa gataacgacc cggtggatat cctcatcacc atggcggcgg tcgatgccaa
     9901 cacgcaccag gaagtgggca tcatgcagat cgtcaacctg tttgaagatg aagaaaattt
     9961 cgaccgttta cgtgcctgcc gtaccgagca ggaagtactg gatctcattg accgcaccaa
    10021 cgcggcagct taagaaggaa ttgaagatgt cattaccgat gttgcaagtc gcgctggaca
    10081 accagactat ggatagcgcc tacgaaacca ctcgcctgat tgccgaagaa gtcgacatta
    10141 tcgaagtggg taccattctg tgcgtgggcg aaggcgtgcg tgcggttcgc gatctgaaag
    10201 cgctctaccc gcacaaaatc gtgctggcag acgccaaaat tgccgatgcg ggcaaaattc
    10261 tttctcgtat gtgcttcgaa gccaacgctg actgggtgac cgtgatttgt tgtgcggata
    10321 tcaacaccgc caaaggtgcg ctggacgtag caaaagagtt taacggcgac gtgcagatcg
    10381 aactgaccgg ttactggacc tgggaacagg cgcaacagtg gcgcgacgca ggtattcagc
    10441 aggtggttta tcaccgcagc cgtgacgcgc aggccgctgg cgtggcgtgg ggtgaagccg
    10501 atatcactgc gatcaaacgt ctttccgata tgggcttcaa agtcaccgtc accggtggcc
    10561 tggcgctgga agatctgccg ctgttcaaag gcattccaat tcatgtgttt atcgccggtc
    10621 gcagcatccg tgatgccgct tctccggttg aagccgcgcg tcagttcaaa cgttccatcg
    10681 ccgaactgtg gggctaagga gcggatatgt tgtccaaaca aatcccgctt ggcatctatg
    10741 aaaaagcgct ccccgccggg gagtgctggc tggaacgcct gcgactggca aaaacgttgg
    10801 gcttcgattt tgtcgagatg tcggtagatg aaactaacga gcgcctgtcg cgcctcgact
    10861 ggagccgcga acagcgtctg gcgctggtga atgctattgt tgaaaccggc gtgcgtgtgc
    10921 cgtccatgtg cctttctgcc catcgtcgtt tcccgttggg cagcgaagat gacgcggtgc
    10981 gggcgcaggg gctggagatt atgcgtaaag ccatccagtt cgcccaggac gttggcattc
    11041 gcgtgatcca gctggcgggc tatgacgttt actatcagga agccaataac gaaacgcgtc
    11101 gtcgtttccg tgacggcctg aaagagagcg ttgagatggc gagccgcgcg caggtgacgc
    11161 tggcgatgga gatcatggat tatccgttga tgaactccat cagcaaggcg ctgggttacg
    11221 cgcactatct caacaacccg tggttccagc tctacccgga tatcggcaac ctgtcggcgt
    11281 gggacaacga tgtgcagatg gagttgcagg ccggaatcgg gcatatcgtc gcggtgcatg
    11341 tgaaagacac caaaccgggc gtcttcaaaa acgtgccatt tggcgaaggt gtggtggatt
    11401 tcgaacgttg cttcgaaacg ctcaaacaga gtggctattg cgggccgtac ctgattgaga
    11461 tgtggagcga aacggcggaa gacccagcgg cagaagtggt gaaagcgcgc atggccaaag
    11521 caggcatggt ggaggcggca taatgcaaaa gctaaaacag caggtgtttg aagccaacat
    11581 ggatctgccg cgctatgtgc tggtgacctt tacttggggc aacgtcagcg ctatcgaccg
    11641 cgaacgcggg ctggtggtga tcaagcccag cggcgttgcc tacgaaacca tgaaagcaga
    11701 cgatatggtg gtggtcgata tgagcggcaa ggtggtggaa ggggagtatc gcccatcttc
    11761 cgacactgcg acgcatctcg aactctaccg tcgttacccg tcgcttggcg gcattgtcca
    11821 tacccactcc actcatgcca ccgcatgggc gcaggcgggg ctggcgatcc cggcattagg
    11881 caccacgcac gctgactact tctttggtga tatcccgtgt acgcgtggat taagcgaaga
    11941 agaggtgcag ggtgagtatg aactgaacac tggcaaagtg attatcgaaa cgctgggcaa
    12001 cgccgagccg ctgcatacgc cggaaattgt ggtgtatcag cacggaccgt tcgcctgggg
    12061 gaaagatgcc cacgacgcgg tgcataacgc ggtagtgatg gaagaagtgg cgaaaatggc
    12121 gtggattgcg cgcagtatta atccacaact caatcatatc gacagctacc tgatgaataa
    12181 acacttcatg cgtaagcacg gtcctaacgc ttattacggg cagaagtaaa acacgcgctg
    12241 cggaaatttc cttcctcggg agataactgg tctaattccg cagccgtttt tcaaaaaaag
    12301 ccccctgcga agggggcaaa gcaaactatg gcaatgtttc gttggttata cctggtgcta
    12361 gcgataaata tccgcgctgg cgtgcatatt gccgttactc cccggttccc gcatcagaat
    12421 tacgtggtag tacgttgcgc cttgcgcatc tgtttcttca tttaatgcct gacgtgcttc
    12481 gctttcagtg gcgaaattat gattgatata aatcacgcct aagctttgca catcgtccat
    12541 atttctggcc tggtggttat taatttcaat ggctgcccat gtatttgcac ttagcaaaag
    12601 cacagccaga agggctaaaa ctcgactgaa catagatacc tcctcgacgg ctgactttgt
    12661 gtgctctcct ctgtgatgat cttctgattt aattttaatc aatgataaag aagttgatgg
    12721 tgaccatttc tgatgcagtt gttcaaaaaa acactgtgac ggaatgtgat gaacttcaaa
    12781 tcagcggttt agaggttatt tgcgaaaggg gagatttatt tctgctccgc ccttgagttt
    12841 agcggggtat gccagtacta taacgcgtca tttttcagcc gacctttaac acgttccttg
    12901 cctccccggg attcggctga cccagacagg aggctgaata atccgtaagg agcaattcga
    12961 tgcgtcatta cgaaatcgtt tttatggtcc atcctgacca gagcgaacag gttccgggca
    13021 tgatcgagcg ctacactgct gccatcactg gtgcagaagg caagatccac cgtctggaag
    13081 actggggccg ccgtcagctg gcttacccga tcaacaaact gcacaaagca cactacgttc
    13141 tgatgaacgt tgaagctccg caggaagtga tcgatgagct ggaaactacc ttccgcttca
    13201 acgatgccgt tatccgcagc atggttatgc gtaccaagca cgctgttacc gaagcatctc
    13261 cgatggttaa agcgaaagac gagcgccgtg agcgtcgcga tgatttcgca aacgaaaccg
    13321 ctgatgatgc tgaagctggg gattctgaag agtaatttct gatgaccaac cgtctggtgt
    13381 tgtccggcac cgtgtgcagg actccccttc gaaaggtcag tccatcagga attcctcact
    13441 gccagttcgt gcttgagcat cgttctgtgc aggaggaagc cggctttcac cggcaggcat
    13501 ggtgtcaaat gcccgttatt gttagcggac acgaaaacca ggccattact cacagtataa
    13561 cggtcggcag tcgcataacc gttcaggggt tcatttcatg ccacaaggca aagaacggac
    13621 tgagcaaaat ggttttgcat gccgagcaga ttgaattgat agattctgga gactagccat
    13681 atggcacgtt atttccgtcg tcgcaagttc tgccgtttca ccgcggaagg cgttcaagag
    13741 atcgactata aagatattgc tacgctgaaa aactacatca ccgaaagcgg taagattgtc
    13801 ccaagccgta tcaccggtac ccgtgcaaaa taccagcgtc agctggctcg cgctatcaaa
    13861 cgcgctcgct acctgtccct gctgccgtac actgatcgcc atcagtaatc ggtcacggtc
    13921 cattaatacg actttgagag gataaggtaa tgcaagttat tctgcttgat aaagtagcaa
    13981 acctgggtag cctgggtgat caggtaaacg ttaaagcggg ctatgctcgt aacttcctgg
    14041 taccgcaggg taaagctgtt ccagctacca agaaaaacat tgaattcttc gaagcacgtc
    14101 gcgctgaact ggaagctaaa ctggctgaag ttctggcagc tgctaatgct cgcgctgaga
    14161 aaatcaatgc actggaaact gttaccatcg cgtctaaagc tggcgacgaa ggtaaactgt
    14221 tcggttccat cggtactcgc gacatcgctg acgctgtaac tgcagctggc gttgaagtgg
    14281 ctaagagcga agttcgtctg ccgaacggcg ttctgcgtac cactggcgaa cacgaagtga
    14341 gcttccaggt tcacagcgaa gtattcgcga aagtgatcgt aaacgtagta gctgaataat
    14401 tcgttattca acgagacgta aaaacgccga ccattggtcg gcgttttgct ttctattttt
    14461 cgtcaggcat taatttcgca agtagatcca ttcaccattc tcttgtgtcc ataagtcagg
    14521 atggtttgct ggcgttagat gcatacgatc actccttatg tctcggtcct gcggcactcg
    14581 ctcgaaacct aaacccgagt agattgcggc tgaatttgat aaggaacgta gaccaatttt
    14641 caaatcatgg ggtaaatcag aatacgcgtt tagcaatgca gcaatgatga atccgagggc
    14701 attagaaacg cctgtaacag tgtgaagttg gtcgatataa agatcgattg ttggtagttc
    14761 agatgaccag caattaggca tttgggcagg aagggttaca acgtatccta tcgtttggcc
    14821 ggtatagtct ggagtactat aaccaggtcc caaaacaacc tcgtgtattg tgtatttact
    14881 gttgggttcg aaggattcga catcttgcaa taaatatttc agttctgcgg ttaacctcgc
    14941 acccgcgtaa gctttattgt atttctgggc gtgaatatta tctaatgtag cttcatggtt
    15001 ccatccaaaa gaattatcac cattcattgt ta
//
LOCUS       DAJTQY010000091        14920 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.091, whole genome shotgun sequence.
ACCESSION   DAJTQY010000091 DAJTQY010000000
VERSION     DAJTQY010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14920
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.091"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(100..525)
                     /gene="arsC"
                     /locus_tag="OO349_003062"
     CDS             complement(100..525)
                     /gene="arsC"
                     /locus_tag="OO349_003062"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HCR5868054.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(538..1827)
                     /gene="arsB"
                     /locus_tag="OO349_003063"
     CDS             complement(538..1827)
                     /gene="arsB"
                     /locus_tag="OO349_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HCR5868055.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(1881..2234)
                     /gene="arsR"
                     /locus_tag="OO349_003064"
     CDS             complement(1881..2234)
                     /gene="arsR"
                     /locus_tag="OO349_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HCR5868056.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKIQAIVRN
                     LARQNCSGDSKNICS"
     gene            2977..3060
                     /locus_tag="OO349_003065"
     CDS             2977..3060
                     /locus_tag="OO349_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HCR5868057.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(3114..4466)
                     /gene="gorA"
                     /locus_tag="OO349_003066"
     CDS             complement(3114..4466)
                     /gene="gorA"
                     /locus_tag="OO349_003066"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HCR5868058.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(4538..5380)
                     /gene="rlmJ"
                     /locus_tag="OO349_003067"
     CDS             complement(4538..5380)
                     /gene="rlmJ"
                     /locus_tag="OO349_003067"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HCR5868059.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSGFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLSWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            5583..7625
                     /gene="prlC"
                     /locus_tag="OO349_003068"
     CDS             5583..7625
                     /gene="prlC"
                     /locus_tag="OO349_003068"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HCR5868060.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAGLAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELEQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDGCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            7633..8385
                     /gene="rsmJ"
                     /locus_tag="OO349_003069"
     CDS             7633..8385
                     /gene="rsmJ"
                     /locus_tag="OO349_003069"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HCR5868061.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(8434..9903)
                     /gene="dtpB"
                     /locus_tag="OO349_003070"
                     /pseudo
     CDS             complement(8434..9903)
                     /gene="dtpB"
                     /locus_tag="OO349_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
     gene            complement(10221..10655)
                     /gene="uspA"
                     /locus_tag="OO349_003071"
     CDS             complement(10221..10655)
                     /gene="uspA"
                     /locus_tag="OO349_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HCR5868062.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            11049..11384
                     /gene="uspB"
                     /locus_tag="OO349_003072"
     CDS             11049..11384
                     /gene="uspB"
                     /locus_tag="OO349_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HCR5868063.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(11455..12954)
                     /gene="pitA"
                     /locus_tag="OO349_003073"
     CDS             complement(11455..12954)
                     /gene="pitA"
                     /locus_tag="OO349_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HCR5868064.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWFSLQFL"
     gene            13186..14385
                     /locus_tag="OO349_003074"
     CDS             13186..14385
                     /locus_tag="OO349_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCR5868065.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQVGRRVLLIDNGKILGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLDLNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVCDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLASIVK"
BASE COUNT         3713 a         3789 c         3928 g         3490 t
ORIGIN      
        1 gaatttgcac ggttttgtag gtcggataag acgcgttagc gtcgcatccg acataaacaa
       61 agcgcacttt accaactatc ggcagtggat atcgccgctt tatttcaggc gcttacccgc
      121 ttcatcaacc actttctcgc catcttcctt ggagaatgcg cctttttgcg catctggcag
      181 aatttccagc accacttctg aagggcggca caggcgagtt cccagcggcg tcaccacaat
      241 cgggcgatta atcagaatcg ggtgctgaag cataaagtcg attaaccgat cgtcagtaaa
      301 tttatcttcc gcaaggccca gctcctcata cggttcgacg tttttacgca gcagcgcgcg
      361 tacggaaatc cccatatcgg caatgagttt gaccagttca tcgcgcgttg gcggagtttc
      421 cagataatgg ataatggtcg gttctgtgcc gctgttgcgg atcatctcca gcgtattacg
      481 cgacgtgccg caggccgggt tgtgataaat ggtaatgttg ctcatatcag tatctcatta
      541 caaagtgaaa gagagacgta gcgccagcgc agccagcgtc acaaacagca caggcagggt
      601 catgataatc cctgtacgga agtaatatcc ccagctgata gtcatattct tctgcgaaag
      661 tacgtgcagc cagagcagcg ttgccaggct accaatcggg gtaattttcg gccccaaatc
      721 gcagccaatc acgttggcat aaaccatcgc ttctttgata acgccagatg ccgtgctgcc
      781 atcaatggac aacgcgccaa ccagtaccgt cggcatattg ttcataatag aagagaggaa
      841 ggcggtgagg aatccggtgc cgagcgtcgc ggcccacagg ccgttatccg ccagcacgtt
      901 gagtacgcca gaaagatatt ccgttaatcc ggcattgcgc aggccataaa ccaccagata
      961 catgccgagc gagaagatga caatctgcca gggggcaccg cgcaggactt tacccgtatt
     1021 aatcgcatga ccgcgtttag cgacgacaaa taatatcagt gcgcccacag ctgcaatggc
     1081 gctcaccgga atgccgagcg gttccaagac gaaaaatccc accagcagaa gcagtaaaac
     1141 aacccagcca gttttgaacg tagcaggatc tttgatcgct tctgcgggag atttcagcag
     1201 cgccatatcg tagttctgcg gaatatcttt gcgaaaatag agatgtagca tcaccagcgt
     1261 ggcaacaatc gcggcgatat ccaccggcac catcaccgag gcgtattcgc gaaagccgag
     1321 gccaaagaaa tcagcggaaa cgatattcac caggttggag acaataagcg gcaggctggc
     1381 ggtatcggca atgaatccgg ccgccatcac gaacgccagc gtcgtgcctt tactgaatcc
     1441 taaagccagc agcatggcga tgacaatcgg cgtcaaaata agcgccgcgc catcattggc
     1501 aaacagggcc gcaacggcgg caccgagcag gacaatccag gtaaacagca agcgaccgcg
     1561 tccattgccc cagcgtgaaa cgtgcagcgc tgcccattca aaaaagccgg actcatccag
     1621 cagcaggctg atgataatga cggcaataaa cgccgccgtc gcgttccaga cgatattcca
     1681 caccaccgga atatcacccg gatgaaccac gcccgtaacc aacgccagta ccgcgccgag
     1741 cgttgcactc cagccgatgc ctaaaccttt cggctgccag ataaccaata cgatggtcag
     1801 gacaaagata gcgcctgcca gtaacataat gcctcccgga taaaacacat gtgcaaatcc
     1861 atatgtgttt agctaaattt ttaactgcaa atgttcttac tgtctccgga acagttttgt
     1921 ctggccaggt tgcggacaat tgcctgaatc ttttcctgtt cacatcgcca ggcttcatca
     1981 ataattttcg ccgcccatgc tggaatatgc ggcgataagc ggtaatgaac ccacttacct
     2041 tgcttgcggt ccagcaatag cccgctttca cgcagcaatg ccagatggcg ggatatcttg
     2101 ggctgcgact gatcgagggc agtgcagaga tcgcagacgc ataactctcc cagttcgcta
     2161 agcagtaaaa cgatgcccag acgggtttca tcagcaagaa ttttgaacaa ttggatgggt
     2221 aacagaaatg acatattgcg ctcctgattg ttgcaggtag tgtctctctt cgaagcggat
     2281 aagtcaaaaa catatatgac ttaacgaatg tgtaagtgca gaggaaggta ataggtgtga
     2341 attttgagtt ggctattcat ttgaaaggcg gtttgaatca ggacgtaaaa aaaccgactt
     2401 tgcgtcggtt ttttactctt cagcctgagt tggtggctct ggctggagtg agaatgccca
     2461 catctaacgg ttcaatacta ttagccagtg gctaacattg caagcgttag cgatttcttc
     2521 agctggagaa gggacagttt gctatacatg gtggtgaaag aggatttata tactttcatc
     2581 gggtcgctgc cgtaccaagc cttgtccgaa tccggacgta aaaaaaccga cttcgcgtcg
     2641 gtttttttac tcttcagccc agagttggtg gctctggctg gagtgagaat gccatcatct
     2701 aacgcatcaa tgctaatacc atactgaaac tattgcaagg ttatgctggt tatttagcct
     2761 aattacactg tatgattatc cagttagctc tgaggcattt tcactctggc aatgcgcata
     2821 aacgctttca aagtcctggt cagaagtacg ggtggtgccg ttaactgatg ctctggccgg
     2881 agtgagattg cccacatcta acaatgaggc atgcgccgtg acaggcagtg gatgagtaag
     2941 cggatgcatt ctcactccat cgcatggaga aaacgggtga ttgataaagc aatcatcgtt
     3001 cttggggcgt taattgcgct gctggaactg atccgctttc tgcttcagct tctgaactga
     3061 tagcggaaac gtaattaagg gctaagagca cactactctt agccctttaa catttaacgc
     3121 atggtcacga actcttctgc cgccgttggg tgaatggcga cggtattgtc gaagtctttt
     3181 ttggttgcgc ccatcttcag cgccaccgcg aagccctgca acatttcgtc cataccaaag
     3241 ccaatgccgt gaataccgac aatcttctct tctggcccaa cgcacaccag cttcatgcgg
     3301 cacggctggc ggtgagtggt gacggcggta tacatcgcgg tgaaagagga tttatacact
     3361 ttcacctgat cgtcgccgta ctgctcgcgc gcctgcggtt ccgttaaacc aacagtacca
     3421 atcggcggat ggctgaagac cacggtcgga atgttgctgt aatccagatg ctcatccggc
     3481 ttgttattaa acaggcgttc agagagacgg cgacccgctg caactgccac cggtgtcagt
     3541 tccactgcac ccgtgttatc gcccaccgcg taaatacctt caatattggt gttttgatat
     3601 ttatcgacga cgatatagcc tttttcgttg gttttaacgc cagcggcttc gaggttgatg
     3661 ttgtcattgg caggctcgcg accaatcgcc caaatcaggc aatccaccgt ttcactgcga
     3721 ccatcttcca gctccagcgt caggctacca tcggcatttt tcactaccgc tttcgggatg
     3781 gcgttggtat gcaactgtgg gccttcggcg ttcatcactt cgaccagcgt ttcagagatc
     3841 atcgggtcga agctgcgcag cggcgcatgt ttacgcacaa acagatgcgt tttcgcgccg
     3901 aggccgttaa tcacgccagc cagctcaacg gcgatgtaac ccgcgccaac aaccgccacg
     3961 cgctctggca aagcaggaag ggcgaagaag ccatcggaat caataccgta ttccacgcct
     4021 ggaatatccg ggtggctcgg acgaccgccg gtggcgatca gaatatgatc ggccgtgatg
     4081 gtttcgccgt taacttccag cgttttggca tcaacgaagc gggcaaagcc tttgattaca
     4141 tcaacgttat ttttaccgag cacgttttca taggaagtat gaatacggtc gatataggcg
     4201 gtacggctgg cgatcaacgt ttcccagttg aacttgttga tggtggtatc gaaaccataa
     4261 tcagggccgt acatatggat cgcttcacgg atttgcgccg cgtgccacat cacttttttc
     4321 ggcacgcagc cgacgttcac gcaggtgccg cccagctctt tggcttcaat cagtgcgcat
     4381 ttctggccgt acatagccgc gcggttgatg gaggcgatac cgccgctgcc gccgccgatg
     4441 gcgatgtaat catagtgttt agtcatgaca tattgtcctt atcgttgatt accgcgattg
     4501 tagcgcgaga cgtaataggt gccagcaatg gctgcaatta ctccggcacg atccagctta
     4561 cggtggcgtg cccggtacct gccggaacca gtttgctgtg cagccacgac agcacgttat
     4621 tcatctgctg ttccagcttc cacggcgggt taatcacaat catgccggaa gcggtcatgc
     4681 cacggcgatc gctgtctggc agcaccgcca gttcaatttg cagaattttg cgaatgccgg
     4741 tcgcttccag gtcgtggatc atgcgcttaa tttgctggcg cagcaccacc ggataccaca
     4801 atgcgtaagt gccagtggcg aaacgtttgt aaccttctgc tatcccgctg accaccgcct
     4861 gatagtcggt tttcatttca tacggcgggt cgataaggat taaaccacgg cgagaaaccg
     4921 gcggcagttt ggccttaagt tgctggaaac cgtcggcttt ttcgacgcgt gcacggctgt
     4981 ctttctgaaa tccagaacgc agcaacgggt aatcgctcgg gtgcagttcg gtcagttgca
     5041 ggctgtcttg ttcgcgcagt agctggcgag caatcaacgg cgaaccgggg tagtaacgca
     5101 actggccgct acggttgaag tgttttacca cattgatgta cgcctccagt tctgcgggca
     5161 aatcgtcctg ctgccagata cgggcgatac cttcgagata ttcgccggta cgctcggcat
     5221 gttcgctacc taactgataa cgcccggccc ctgcgtgggt gtcgagatag agaaacggtt
     5281 tatctttctc tttcagcgac tcgatgatca ggctctgaac ggtatgttta aggacgtcgg
     5341 cgtggttgcc agcgtgaaag ctgtggcgat aactgagcat gggtaaaggt gttccggtaa
     5401 gtaaaaattg gcccgtattc ggggcgcaca aaagcctatc aggacagtat accgaaaaga
     5461 ggccgccgcc gcgaaagcgt aacgtttctc attgaaattc actacactta accccatgct
     5521 acacacatta tgtaaagcgc ctgttgagcg cttccttaac ctctttaacc aggactgcgc
     5581 taatgacgaa tccgttactg actccctttg aattgcctcc gttttctaaa attctcccgg
     5641 aacatgtcgt tccagccgtg actaaggcat tgaacgactg ccgcgaaaat gtggagcgcg
     5701 tagtagcgca aggggcaccg tacacctggg aaaatctctg ccagccgttg gcggaagtgg
     5761 acgatgtttt ggggcgtatc ttctccccgg tcagccacct gaactcggtg aaaaatagcc
     5821 cggaactgcg tgaagcgtac gaacaaaccc tgccgctgct gtcggaatac agcacctggg
     5881 tagggcaaca tgaagggctg tataaagcgt accgcgacct gcgcgatggc gatcattacg
     5941 ccacgctgaa cacggcgcag aagaaagcgg ttgataacgc cctgcgtgat tttgaactct
     6001 ctggcatagg tctgccgaaa gagaaacagc agcgttacgg cgaaattgct acccgtcttt
     6061 ctgaactggg caaccagtac agcaacaacg tcctcgatgc gacgatgggc tggaccaaac
     6121 tcgttaccga cgaagcgggg ctggcgggga tgccagaaag cgcgctggct gcggcaaaag
     6181 cccaggccga agcgaaagag ctggaaggct acctgctgac gctggatatc ccaagctacc
     6241 tgccggtaat gacctactgc gacaaccagg ctctgcgcga agagatgtat cgcgcttaca
     6301 gcacccgcgc ctccgatcaa ggcccgaatg ccggtaagtg ggataacagc aaggtgatgg
     6361 aagagatcct cgcgctgcgt cacgaactgg cgcaactgct gggctttgaa aactacgcct
     6421 ttaaatccct tgccactaaa atggcagaaa acccgcagca ggtgctggat ttcttaaccg
     6481 atctggcaaa acgcgcgcgt ccgcaaggcg aaaaagagct ggagcaactg cgtgccttcg
     6541 ctaaagccga atttggtgtc gatgagttgc agccgtggga tatcgcttac tacagcgaaa
     6601 aacagaaaca gcacctctac agcatcagcg acgagcagct gcgtccgtac ttcccggaaa
     6661 acaaagcggt taacggcctg tttgaagtgg tgaaacgtat ttacggcatc accgctaaag
     6721 agcgtaaaga tgttgatgtc tggcatccgg atgtacgttt cttcgaactg tatgacgaaa
     6781 ataacgaact gcgcggcagc ttctacctcg atctgtatgc ccgtgaaaac aagcgcggcg
     6841 gggcgtggat ggatggctgc gtaggccaga tgcgtaaagc tgatggttct ctgcaaaaac
     6901 cggtcgcgta tttgacttgt aacttcaacc gcccggtaaa tggtaaaccg gcgctgttca
     6961 ctcacgacga agtgatcacc ctgttccacg agttcggtca cggcctgcac catatgctga
     7021 cccgcatcga aaccgctggt gtttccggta tcagcggtgt gccgtgggat gcggtcgaac
     7081 tgccgagtca gtttatggaa aactggtgct gggagccgga ggcgctggcg tttatctctg
     7141 gtcactatga aaccggcgaa ccgctgccga aagagttgct ggataaaatg ctggcggcga
     7201 agaactacca ggcggcgctg tttattctgc gtcagctgga gttcggcctg tttgatttcc
     7261 gccttcatgc cgagttccgc ccggatcagg gggcaaaaat cctcgaaact ctggcagaaa
     7321 tcaagaaact ggttgccgtg gtgccatctc cgtcctgggg ccgtttcccg cacgctttca
     7381 gccatatttt cgccggtggt tatgcggcag gttactacag ctacctgtgg gctgacgtac
     7441 tggcggcaga tgctttctcg cgctttgagg aagagggcat tttcaaccgt gaaaccgggc
     7501 agtcgttcct cgacaacatt ctgagccgtg gcggttcaga agagccgatg gatctgttca
     7561 aacgcttccg tggtcgtgaa ccgcagctgg atgcgatgct ggagcattac ggcattaagg
     7621 gctgatcatt cagtgaaaat ctgcttaatt gatgaaacag gcgccggaga cggcgcctta
     7681 tctgttctgg cggcccgctg ggggctggag cacgatgaag acaacctgat ggcgctggtg
     7741 ttaacgccgg aacatctgga attgcgcaag cgtgatgagc caaaacttgg cggcatcttt
     7801 gttgattttg ttggcggagc gatggcgcac cgacgcaaat tcggcggtgg tcgcggtgag
     7861 gcggtggcga aagcggtggg cattaaaggc gattatttgc cggatgtggt ggacgccacc
     7921 gcagggctgg ggcgcgatgc ctttgtactg gcttcagtcg gctgccgcgt gcggatgctg
     7981 gagcgtaatc cagtggttgc cgcgctactc gacgacggcc tggcgcgtgg ttatgcggat
     8041 gcggaaatcg gcggctggtt gcaggagcgg ttgcagttaa ttcacgcctc cagcctgacg
     8101 gcgctaaccg atattacccc acgcccgcag gtggtctatc tcgatccgat gttcccgcat
     8161 aagcagaaaa gcgcgctggt gaaaaaagag atgcgtgtct ttcagtcgct ggtggggccg
     8221 gatcttgatg ccgatggatt actggagcct gcacgcttgc tggcgaccaa acgcgtggtg
     8281 gtaaagcgcc cggactacgc gccaccgctg gcgaatgtcg ccacgccaaa cgcggttgtg
     8341 actaaagggc atcgctttga tatctatgca ggtacgccag tgtaaaagta aagcagggta
     8401 gcgataacgc taccctgctt tctgcaagaa taattaatgg ctttccggcg tcgcaatcat
     8461 gcgtttcagc cacggcacca tcaacagcat cactactgca acgcccagcg tgaccagacc
     8521 aatcttacca aacacgttgg tatagacggg caacgtctca agcggatcgg taatgttgtc
     8581 cggcaccgcg gtaaatgttg ccacatagcc gcccagcaag aacgcggcag cctgcgtcag
     8641 gaaccacatc ccgagaataa agcccatcaa atgctgcggc accagggcag caatcatcgc
     8701 caggccaagg gcgctaataa acaattcacc caggctctag aataaataca ccagcacgat
     8761 aaaccatggc gatgtcagcc cctggtcatc cgcaaaccac attcccgcag cagcagccgt
     8821 caaaaagccc agtgagcaca taaacatgct gagagtaaat ttcatcggca tcgagaggtc
     8881 tttgcctttg ttacccagat gcgtgtaaat gcctgccagt attgggctgg cgagtaccac
     8941 ccagaacggg ttaagcgcct ggaagctgac cgggttgatg gaaaaaccga gaatttcatg
     9001 atgcacgttg ttgatggcaa agaagttcag cgatgttggc atctgggcgt agagaatgta
     9061 aaacaccacc gcttcgagca tcaggacaaa ggcgacaaac attttattgc gcccggtttt
     9121 atccagcttg aatgcctgac gaaagaagat gatagtgacg acgatggaga gaacaatcag
     9181 caccagattg gcgacttcta cgttgtgcat cagccatgca catacgaaga tcatcaccac
     9241 gctgccaagt aatacgtaca acagtttgct gaagctcatc ggcttgaagt cgggttcaga
     9301 accaatgtct ttcaccattc cacgacaggc gatgtaaacc agtaatgcga taattaaccc
     9361 cgccccgcac aggttgtagg tgactgaata accgaatcta tcagcgatca caggggccag
     9421 cgataacgct atcaacgagc cgatattgat cgacatatag aacagggtga atgcgccatc
     9481 aagccgcgga tctttcggcg gatagcactt cgaaagcaag ctggctgggt tagctttaaa
     9541 caggccgtta ccgacagcga tagtccccag ggcgatgaaa atcaggtcag gcttaagtag
     9601 cgacatgccg gtcatgaagt agccaatcgc cagcacaagt gctccaagaa caatggtgcg
     9661 tttggtcccc aacaggtggt cgccgacata gccgccaatg gaaatgaggc catagaccag
     9721 tgcagcaaaa gcaccaaaag tgacaaaagc ctgctcttgc gagaatccaa gctgtttaac
     9781 gaagaaaacc gccagtacgc cctgcacgcc gtagtagccg aatcgctccc ataactcgac
     9841 aaaaaagatc atgaaaaatg ggcgaggttg ctgcagcatc cccatgggtg ttgttgtatt
     9901 aatatttatt aaccttccaa taccatcctg aaaagtatga acgcgtaagg gataacgcgc
     9961 gaaaaagttt acatctgtca gagattctta atattgatgc ggtaaataga ttaatacact
    10021 attacaacag aaaataacca gctgatcaca ctaatgatgg aaaatatgat ggtgttaaat
    10081 tttgtattgg caggagattt tgattgcgta cgcagatcgt aggccagata aggggtttac
    10141 gctgcatcag gtaacaaggc gcgccaaaaa gcaaaacgcc cgcatatgcg ggcgttcagg
    10201 aacacgtcgt ggagggatga ttattcttct tcgtcgcgta gcggaacaat cagcatatca
    10261 acgtgaacgg tgttgatcag ctgacgtgcg gaagacatca gtttgctcca gaagtcctgg
    10321 tggtgaccac aaaccaccag atccatatcg tatttcttga ttgcatcgac cagaacctgg
    10381 cctaggtcgc cgctgccgct cagggtttca gtgattggat agcctgcatt agtggaaagc
    10441 tcggtcagcg catgatgtgt ctcttcagag atgcgtttct gcatatcacc cagattcaca
    10501 tcaataagcc cggtgtatag gtcagagtag tttacatcta cgtggatcag agaaactttc
    10561 gcattgtagg ggcgagccat agagactgct ttctctacca gaactttgct ttccggggag
    10621 agatcgactg cgatgagaat gtgtttataa gccatagtgt tactccttcc ataaagttgt
    10681 cgatgactgg ccagctagcg tttcttgtgc cgttacgtca cccgcgtcct gcgaactacg
    10741 tgcgcggggc taacccagcc gtaaagattc agtgttaaga ccatccttac cttatagcga
    10801 cccggatgat ccgtcaatcc gccttgctta cccgttagat aaacaaattt tcgacatgaa
    10861 tattatattg atagtggtta accttctgga aaaaaaacaa cctgatctcc tacactatct
    10921 atagagccgc tcggatgttg gtcaccacaa aagcggtttc tccaggacct ggtgttaact
    10981 gattggcagc gtatcgggag cggtagcccc ggggtggaac tccgcgggca ggtcgccggg
    11041 gaggaggtat gataagcacc gtcgcattat tttgggcttt atgtgtcgtt tgcattgtta
    11101 acatggcgcg ctatttctca tcactacgcg cgttgttagt ggtactgcgt aactgcgatc
    11161 cattgctcta tcaatatgtt gatggagggg gcttttttac ctcacatggc caacccaaca
    11221 aacaggtgcg tctcgtttgg tatatctatg cccaacgtta tcgcgatcat cacgatgatg
    11281 agtttattcg ccgctgtgag cgggtgcgtc ggcagtttat tctgactagc gcattgtgtg
    11341 gtctggtggt ggtcagcctg attgcattga tgatttggca ttaagcactt cagcaataat
    11401 aacgtataaa aaaagcgggc cagtaaactg acccgctcgt tttggtgcgt acgattacag
    11461 gaactgcaag gagaaccagt acagcccgcc ggaaagtagt accgcagccg gaagggtaaa
    11521 cacccaggcc atcagaatac tggttacggt tttacgctgt aagccgccgc catctaccac
    11581 catcgtcccc gcgacagaag aggagagtac gtgagtggtg gaaaccggca tcccggtata
    11641 actcgccagg ccgattgaca ctgccgccgt catctgggca gacatcccct gagcgtaggt
    11701 catgcctttc ttaccgattt tctcaccgat agtcgttgcc acacgacgcc agccaatcat
    11761 cgtaccgata cctaacgcca gcgcgaccgc catgatgatc cacaccggcg catactcgat
    11821 ggtgctaagc atgtcggact tcagtttctt caacaggcgc tgatcgtcag cactcacacc
    11881 aggcatcttc accactttgt cgatagtgtc agaaacgcac agcataatgc ggcgcatctg
    11941 gctacgttga tcaagcgaca gcttgtcgta gctttccaca tcggtggtca acataccttt
    12001 caggcggttg agcgcgttaa tggtattcga cggatggcag tggaactccg caggttgcgt
    12061 tgcgccagct tccggagccg gtactaactg atcagcaccg gtagcctgtt tgagcagcgc
    12121 aggatgctgc tcaaagtaag cttcgacgtt gttgatggca tcacgggtac gggtgatttc
    12181 gtagccagtg gcattcatgt tcaccacgaa gcctgctggc gcgacgccaa tcaataccaa
    12241 cataaccaga ccaatgcctt tctgaccatc gttcgcgccg tgcgaaaacg ccacgccgat
    12301 agcggaaagg atcagcgcga tacgcgtcca gaacggcggc tttttcttgc cgtctttctt
    12361 ttcacgctcc gctggggtca ggtggatacg ggcgcgtttc ttggtgccgc tccagtagcg
    12421 acgcagcaag aaaatcagac cgccagcaaa caccaggccg acaatagggg aaacgatcag
    12481 agaaccgaaa atacttaata ctttcgggat attgagtgca tccaccactg acgtcccggt
    12541 catcaacgca ttggttaaac caatcccgat gatcgcgcca atcagcgtat gagagctgga
    12601 tgcaggtaaa ccaaagtacc aggtacccag gttccagata atcgccgcca gcaacataga
    12661 gaacaccatg gcaaggccat gagacgatcc catattaagc agcagatccg tcggcagcat
    12721 atgcacaatg gcataggcaa cactcagacc acccagcaaa acacccaaaa agttgaacac
    12781 cgccgccata accacggcga gctgagaacg catcgcgcgg gtatagataa cggttgccac
    12841 ggcgttggct gtgtcatgga aaccattgat ggcttcgtag aacagcacaa aagccagtgc
    12901 aagcaataat aacagcccgg tatgcaaatc caggccagca aacaaatgta gcataggacg
    12961 ttacgccatt ttgaggacat gaacgcggcg cattatcagt gactttcgcg gcgcgggcaa
    13021 agtgaaatat agactttttt tgatttgcct cctgtatgga tttcactcaa aaaataatta
    13081 tcttatataa ttcaggcaaa tacttccttt tagtaatatt gatgctggtg cgaccactga
    13141 ggaatcttta caattcacgc ccgttttttc taagaggagc gcaacgtgga aaggtttgat
    13201 gccattatta taggcgctgg tgcggcgggt atgttctgtt ctgcgctggc aggtcaggta
    13261 ggacgccggg ttctgctgat cgataatggt aaaatactag ggcgcaaaat ccttatgtct
    13321 ggcggtgggc gctgcaactt taccaacctt tatgtcgaac caggcgctta tctgagccag
    13381 aatccgcatt tttgtaagtc tgcactcgca cgttttaccc agtgggattt cattgatctg
    13441 gtcaataaac acggtatcgc ctggcacgag aaaacgttag ggcaactctt ctgcgatgac
    13501 tccgcgcagc agattgtcga catgctggtg gatgagtgcg agaagggcaa tgtaaccttc
    13561 agattgcgta gcgaagtgct gagtgtggcg aaggatgaaa caggcttcac gcttgatctg
    13621 aacggcatga ctgtcggttg cgaaaagctg gtcatcgcga ccggtgggct gtcaatgccg
    13681 gggctgggcg catcgccgtt tggttataag attgccgaac aatttggcct caacgtgctg
    13741 ccgacccgcg cgggtctggt gccattcact ctgcataaac cgttgctcga agagttacag
    13801 gtgctggcgg gcgtggcggt gccttccgtg attaccgctg aaaacggcac cgttttccgt
    13861 gagaacttac tcttcaccca ccgcggctta tctggaccgg cggtgttgca gatttcaagc
    13921 tactggcaac cgggtgaatt tgtcagtatc aatctgctac cggatgtgga cctcgaaacc
    13981 ttcctgaatg agcagcgtaa cgcacatccg aatcaaagcc tgaaaaacac actggcggtt
    14041 catctaccga agcggttggt tgaacgctta cagcaactcg ggcaaatccc ggatgtttcg
    14101 ctaaaacagc tcaacgtgtg tgaccaacag gcactgatta gcacattgac cgactggcgc
    14161 gtacaaccca acggcactga aggctatcgc actgccgaag tgacgctcgg cggcgtggac
    14221 accaacgaac tttcttcacg gacgatggaa gcgcgcaaag tgcctgggct gtacttcatc
    14281 ggcgaagtga tggacgttac cggctggctg gggggctata acttccagtg ggcgtggtcg
    14341 agtgcatggg cttgtgcgca ggatttggcg tcaattgtga aataaccatg acgttttccc
    14401 gtcagccgtt tgtatatgtc gttattaagt cattttggta cggttctgca tccgacattc
    14461 tggtgttaac tcgctgtttt gttagcgaat taacgctatt gccgttgtgg ttattttata
    14521 gtgtatttat tctcagagaa tatatattac tgaaataaaa aacacaaaga ataaccaaag
    14581 gaaattattt gaaacgtaaa aggtatatat ttttaatctg taaaatttaa aatcacctcc
    14641 tctggattga atcaacttgc tctatttaag acgattgttt ttacattttc accacctgta
    14701 tcaggtggtg tttttttatt ggcggcaatg cctttatctt atttttatta cgttgtaaat
    14761 atggctattc ctaaatgaaa tttgatttgc tcagtttatt tgtgatgtca ggggagtgct
    14821 tgccatgaga gtcgtgatat tattattcat catttttagg taatgctgcc aacttactga
    14881 tttagtgtat gatggtgatt ttaaggtgct tgcgtggctt
//
LOCUS       DAJTQY010000092        14565 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.092, whole genome shotgun sequence.
ACCESSION   DAJTQY010000092 DAJTQY010000000
VERSION     DAJTQY010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14565
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.092"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            <1..581
                     /locus_tag="OO349_003075"
     CDS             <1..581
                     /locus_tag="OO349_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CbrC family protein"
                     /protein_id="HCR5868066.1"
                     /translation="GALTFKYHPKPLETGAFEQDKTVECDCCEQQTSVYYSGPFYCVD
                     EVEHLCPLCIADGSAAEKFAGSFQDDASIEGVEFEYDEEDEFAGIKNTYPDEMLKELV
                     ERTPGYHGWQQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGIRNSDLA
                     KCLQKGGDCQGYLFRCLHCGKLRLWGDYFVVI"
     gene            complement(635..1357)
                     /locus_tag="OO349_003076"
     CDS             complement(635..1357)
                     /locus_tag="OO349_003076"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCR5868067.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASV
                     LQLHPSLMVIADKAAAAELAFG"
     gene            complement(1359..2530)
                     /locus_tag="OO349_003077"
                     /pseudo
     CDS             complement(1359..2530)
                     /locus_tag="OO349_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709476.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
     gene            complement(2557..4173)
                     /gene="bglH"
                     /locus_tag="OO349_003078"
     CDS             complement(2557..4173)
                     /gene="bglH"
                     /locus_tag="OO349_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HCR5868068.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMIDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTHTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIARNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNNIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRLEIRFYATYI
                     KALENELDGFTFEDNKDAQFAVGAQAEIWW"
     gene            complement(4259..5653)
                     /gene="bglB"
                     /locus_tag="OO349_003079"
     CDS             complement(4259..5653)
                     /gene="bglB"
                     /locus_tag="OO349_003079"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HCR5868069.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWLEDGKGITTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYYRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMLYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKAYHSLIP
                     EAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQIDPVGLRVLLNTLWDRYQKPLFIVENGLGVKDSVEADGSIQDDYRIAYLNDHLVQV
                     NEAIADGVDIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKKSFGW
                     YAEVIKTRGLSLKK"
     gene            complement(5672..7549)
                     /gene="bglF"
                     /locus_tag="OO349_003080"
     CDS             complement(5672..7549)
                     /gene="bglF"
                     /locus_tag="OO349_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HCR5868070.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFGFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADAPGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISEMIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHRGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTLMLH
                     FITSKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(7683..8504)
                     /gene="bglG"
                     /locus_tag="OO349_003081"
     CDS             complement(7683..8504)
                     /gene="bglG"
                     /locus_tag="OO349_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HCR5868071.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRLISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMHLVSAQ
                     MSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILEHASIN
                     DSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRKEH"
     gene            complement(8791..9516)
                     /gene="phoU"
                     /locus_tag="OO349_003082"
     CDS             complement(8791..9516)
                     /gene="phoU"
                     /locus_tag="OO349_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HCR5868072.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            complement(9531..10304)
                     /gene="pstB"
                     /locus_tag="OO349_003083"
     CDS             complement(9531..10304)
                     /gene="pstB"
                     /locus_tag="OO349_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HCR5868073.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSCADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(10396..11286)
                     /gene="pstA"
                     /locus_tag="OO349_003084"
     CDS             complement(10396..11286)
                     /gene="pstA"
                     /locus_tag="OO349_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HCR5868074.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(11286..12245)
                     /gene="pstC"
                     /locus_tag="OO349_003085"
     CDS             complement(11286..12245)
                     /gene="pstC"
                     /locus_tag="OO349_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HCR5868075.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(12332..13372)
                     /gene="pstS"
                     /locus_tag="OO349_003086"
     CDS             complement(12332..13372)
                     /gene="pstS"
                     /locus_tag="OO349_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HCR5868076.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(13620..>14565)
                     /locus_tag="OO349_003087"
     CDS             complement(13620..>14565)
                     /locus_tag="OO349_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5868077.1"
                     /translation="GYLITANENKVGTTFNTTVTNGSSYLGRCHCDTGNVGEFPYIYY
                     TSKINQALTYAGVHSNINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYN
                     CNRIEPLSISSGAKAIVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADV
                     YIRGDITAPQSCEINNLKPVCFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLGI
                     LNTDDISTSFYATEPNTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMDKSTVY
                     GEKSGSVTFSAAPASLTGARPAPGQFTATATITVEIVR"
BASE COUNT         3757 a         3718 c         3316 g         3774 t
ORIGIN      
        1 tcggggcgct tacattcaaa tatcatccca agccactgga aacaggcgca tttgaacagg
       61 ataaaaccgt agagtgcgat tgctgtgaac aacagacgtc agtttattac tcgggtccct
      121 tttattgcgt tgatgaagtt gaacatctct gtccgttgtg tattgcggac ggttctgctg
      181 cagaaaagtt tgcaggtagt tttcaggatg atgccagcat agaaggtgtt gaatttgagt
      241 atgatgaaga ggacgaattt gccggtatta agaacacata tcctgatgaa atgctgaaag
      301 agttggttga acgcacgcca ggttatcatg gatggcaaca ggaattctgg cttgcgcatt
      361 gtggcgattt ctgtgctttt atcggctatg tgggctggaa tgatataaaa gatcgcctcg
      421 atgaatttgc caaccttgaa gaagattgtg agaatttcgg tattagaaat tctgatctag
      481 ctaaatgcct gcaaaagggt ggtgattgtc agggttatct cttccgctgt ctccactgcg
      541 gcaagctgag actgtggggt gattatttcg tagttattta aataatgaga acaggccgga
      601 gcgtaattca tacatccggc cttatttctt cagcttaacc gaacgctaat tctgcggcag
      661 cggctttatc agcgattacc atcagcgaag gatgcagctg caacacggaa gctggaacat
      721 cttcggtaac cggaccttgc agcacatttt tcagtgcctg cgctttacct gcgccactga
      781 caattatcag caagtttttc gccgccatga tgcttttcgg ccccatcgtc acgtagctgt
      841 caggcactaa cgaaaagtcg ccgcccaact caccatgcgc cacaatatca accatctctc
      901 cctggatcgg gaactccacg gtttgctcat ggaaatgggt cgtattcggc aaattgccac
      961 aaaaatgacc atctgcacct aatcccagca ccaccaaatc caggccgcct tcacgcgcca
     1021 gtttctgatc atgctcgcgg tagttatcaa tagtgagctt ctggatattc tcttctttga
     1081 tccccgcagg ggtgaaaaac agattacgca gattggtaat cgttacgcct tctccctctt
     1141 tgccgcgaaa gggaatttca tcgaaattat agaaatagca gttatcgtac caaggcttac
     1201 ctttaaccag ggtggtgagg tattcataca tgcctttggg cgtgctaccg gcggtaattg
     1261 ccaggttaac acgacgcgtc ttcgacatat aaccaagcag atggtgtgcc gcgacacggc
     1321 tcatttcctg gtaatcttcg gtaatgatta atttcatctt aatgccttat tttaaatttc
     1381 tgtacgaagg ctgcataggc aggccgccag acatccattt catggttcag gccgggatat
     1441 tcctggtaat caaacttaat ttttttctgc tcaagctcag ttttcagccc ggcgatatcc
     1501 ttgccggtta cgacatcttt atcaccaacg accacagtaa aattacgtag ttgctggtta
     1561 atagctgccg gatcgttcag ccgggccgcg acaccttcat ccggtacggt tgtcgtggta
     1621 acaccactga atgtggccag ccaaagcttt ccagatgatt cattccggaa accagcgcct
     1681 ggtacccgcc ttgtgaaagc cctgccagcg cgcgaccatc ggcatcttta cggacactaa
     1741 aacgcttgct aatcagcggg ataatatcgt tcatcagttc gcgatctgcc gctttagcat
     1801 tcagcggata aaagacttta cgtctttcct gaggcacgaa atcttcggga ataatgccct
     1861 tcgcatcggt ttcggtatcc gggatcacca ccagcatcgg tttaattttc ccttcagcca
     1921 gcaggttatc catgatttgc gggatacgcc cctgatcgat agcggaacgt ccggtatcac
     1981 caaaaccgtg atagaaatag agcactggca aaggctcgcc catgccggtg tatcctggcg
     2041 gggtccagac atacatctga cgttcagatt gcaatgcgtt ggagtggtaa gttatggcga
     2101 tcaaatcacc atgcgcaaca gaacgcgtat ccagataact gcctggcacc agaatcatac
     2161 tggagttaac ctggcgctga ggcttagtca ttgcggtgcc tgtatcaatg ctgcgtacac
     2221 catcaacatt gaaaaaatac tcgtacaggt tgcctttcag gataggtgtg cgccacgacc
     2281 agactcctgc ttcgtctttg gtcatcgggt gaatattgtc cggaaccgga acacccacca
     2341 ctacagagac attttttgcc ccaggggcaa agtagcgaaa ggtcacgctg ttatcggcat
     2401 tgacctgagt cacatactgt ttcaccggga tagtgggtgc cgggctggct ggcatatccg
     2461 ctgctatggc ccactgagca gaaataccgc tggcaatagc cagcgttaaa gcagcaattt
     2521 ttatattcat cgtaatattc ctttcattaa gttccattac caccagattt cagcctgggc
     2581 accgacagca aactgggcgt ctttattgtc ttcgaaggtg aagccatcca gttcgttttc
     2641 cagggctttg atatacgtgg cgtagaaacg aatttccaga cgcgaggtca acatactggt
     2701 attgacttta aaggtatgga agagcgtggt tttataacca gactcattaa atttattact
     2761 attcgcatct ttgttttgct gggtgaaata gcccagttca acacctgtct ggttatattg
     2821 gtcccaaata taggccgggc gaacaaccgc acgaatagat tcgaaatcag agtgtgcgcc
     2881 tgtttcgtag ctataaatat tgttaccgaa ggagtaaaca atcgcgttag ccacaataaa
     2941 atgatcgccg atataggctt cgccctgcga ggtcagacga actgctgttc cgccggtgtg
     3001 atcaccataa taacgaccat taaaggtggt aaatggactt gcgccagcat aacggccaaa
     3061 gttcctggcg atagagttat tcgcaaccag gaaggagaat tcgttgaagc cacctttatc
     3121 aaatttctgc gttaaagatg tgccgaacat ccaggtatct ttccagtcat aatacccgtt
     3181 attatcctga ttatcttttt ccgatgcgct ttcgtttgcc gtgacataac gaccactcac
     3241 cattaaggtc gctttatccc ataacgggat atctttatag cgtaaatcaa tagtatgggt
     3301 attaatctgc tgtttgtttt gcaggctgcg atcgtaatca tcgacatctt cgcgaaccag
     3361 cgcgatatca attttacccg gaccgacttt ccagttttcc agacctacac ccgctgcagc
     3421 atcagtacgc tgcgttttcc agtccagcat ctggatttca attttcggcg caccgtgttt
     3481 acccacccag aaatcagcct ctggcgcaaa gggcaggaaa cctttggtgg taacgtacat
     3541 atcggagaac tgcataaagt tctcgccacc ggcattatcg ccaaaccagc cggtagagta
     3601 ctgctgacca acgttaccat ctatcatcac aacggcatca acccgtttgc cgttttcgtt
     3661 gtagacacgt tgttttaact gcaaatcaaa ccagccggag tattcgttac caaagcggcc
     3721 cagagaacca atcgcccatg atttaggtga accatgagag gcggtccccc aaccagaacg
     3781 gtaatagccg ttgtaactaa agccaatttc atctttcaca aacttactga aatctttcag
     3841 agtcatggaa gaataaatcg agcctgtctc actggcattt ttctcttcat ttttgaccag
     3901 tacagcatca ggttttgcgg cactactggt cgggaacgga ttggcggcat acccttgatc
     3961 attcgtactt acgctacgat tcaccgtcgc tggcgtatat tttttcttct cttcatcttt
     4021 atactttttg agttcgcttt gcgtttctct taacgctttt tctaataact caaggcgttg
     4081 ttccaccgtt aatgattcag ccaatgattg tgcagaaaag gctaacggtg ccattaataa
     4141 gatggcagag gtaataagat ttcgtctaaa cataatatcc ctttatggtg caaagaaaga
     4201 attaacgtat cgcatccaga ctgttctgaa tgcgacgata attaaggtgc tttattggtt
     4261 atttttttaa tgacagcccc cgcgttttga tcacttctgc ataccatccg aagcttttct
     4321 tacgcgttct tgtgaggctt ccttcgccat tatcatcacg atccacataa ataaagccgt
     4381 agcgcttaga catttgtgaa tgagatgcac tgactaaatc aattggcccc caactggtgt
     4441 accccataat atccacacca tcggcaatcg cttcatttac ctgtaccagg tgatcgttta
     4501 aataggcaat tcgataatcg tcctgtatcg aaccatccgc ttcaacgctg tcttttacgc
     4561 ctaatccatt ctcgacaata aataacggtt tttgataacg atcccaaagc gtatttaaca
     4621 gaacccgtaa tccaaccgga tcaatttgcc acccccactc tgaacttttc agatgcggat
     4681 tggggatcat attcagtatg ttgccctgcg catttttatt aatgctttcg tcgtgggaaa
     4741 cacaaccagt catgtaataa ctaaaagaga tgaaatctac agtatgtttt aaatcttctg
     4801 cgtcactttc agtcatctca atggtgatat tgtggtcgcg gaagaaacgc tgcatatagc
     4861 cgggatattg gccacgcgcc tgaacatcac caaagaacat ccagcgccgg ttctcttcca
     4921 tggcctgcaa catatcctgt ggctggcagg tgagggggta aaccagccca ccgagaagca
     4981 tattgccgat tttcgcttcg gggatcaggc tatgataggc tttaactgcc cgcgcactgg
     5041 caaccagttg atgatggatc gcctgataaa cttccgcctc gccactctct tctgccagcc
     5101 ccacgcccgt gaatggcgcg tgtaacgaca tattgatttc attaaacgtc agccataacg
     5161 ctactttatg ttggtagcgg gtaaagaccg tgcgggcgta atgctcgaag tgatcgatga
     5221 ccgcccgatt agcccaaccg ccgtagtttt tcaccagccc atatagcatt tcgtaatggg
     5281 ataacgttac cagcggcttg atccccgcct gcgccatttc atcaaacagc cgatcgtaaa
     5341 acgctaaccc cgcttcattc ggttcggctt cgtcgccctg agggaaaatt cgcgcccagg
     5401 caatggaaat acgcagacag gtgaagccca tctcggcaaa taacgcgata tcttccgggt
     5461 aacggtaata aaaatcgatg gcgacatctt tgatattctc tttccccagg atgcgcggtt
     5521 ccatttttcc cattacgcca tgaggctgta aatctgaggt cgtgatccct ttgccatctt
     5581 ccagccaggc accttccacc tgattggcag ctgttgcacc accccaaaga aatgtttctg
     5641 gaaatgcttt cataattaac tccttttatc gttagcgaat gatggataac agcggttcac
     5701 ctgcgcttat ctgcgccgtg ccgtggggta atacgtctgt aaaatcatcg ctattactga
     5761 ttaataccgg cgtcgtcaga tcaaatccgg cctcgcgaat agcagggata tcaaaagaaa
     5821 tcagccgatc gcctgtattg accttgtcac ccacgttgac gtgagcggaa aagaatttgc
     5881 cgtccagttt tacggtgtcg ataccgacat gaatcaggat ctccacacca tcatctgact
     5941 caatgccaat ggcgtgtaat gtggcgaaca acgaagcaat tcgacccgca accggagaac
     6001 gcacttcacc aaccgagggc agaatggcga tacctttacc caacaggcca ctggcaaacg
     6061 tggtatcagc gacgtgaatg agcggcacaa tctctcccgt catcggtgaa cagataccgc
     6121 cctgctcagg tggtgtaata acctctggtg ttttctcttg cggggcaccc tgcgctggct
     6181 gacgtttact ggtgatgaaa tgaagcatca acgtaccaac aaatgcgcaa cctatggcaa
     6241 tgacaccgcc aataacgctg gcccagacgg tgaaatcaat ccccgttgac gggatggtct
     6301 gcatgaaggt gaaaatactt ggcaaaccaa aggagtaaac tttcgtttgc gcgtagccaa
     6361 tgatggtggc ccccaatgct ccactgatac aggcgataac aaaggggtac ttacgcggca
     6421 ggttgacgcc atataccgct ggttcggtga taccaaacag acccgtcaac gccgctgatc
     6481 ccgccaccac ttttttctgc gcatcgcgtt cgcagaggaa gacgccgagc gccgccccga
     6541 cctgcgccat aatggcgggc attaacagcg ggatcatggt gtcgtagccc agcacggtga
     6601 agttattgat acacagcggc accaggcccc tgtgcagtcc gaacatgacg aagatttgcc
     6661 agaagccgcc cattaccgcg cccgcaaatg caggaaccgc ctgataaagc cagagataac
     6721 cggcggcaat catttcgctt atccaggttg atagcggccc caccagcaga aaggtgacgg
     6781 gtgtgataac catcagacat agcaatggtg tgaagaaatt tttgattgcc gacggtaacc
     6841 acgcattaag tcggcgttcc agaatgctgc acaaccaggc agaaaaaata atgggaataa
     6901 ccgatgacga gtaattcaac aatgtgaccg gaatacccag gaaatccagc cccggcgcat
     6961 ccgctttttg cccgttctcg aaggcagtca gaattaatgg atgcactaac gctccaccaa
     7021 tcaccatggc agtaaatgga ttaccgccga agcgtttgcc cgcggtgtat cccaggatta
     7081 tcgggaagaa cccaaacaag gcatcactgg cgctgaataa aattaaataa gtaccacttt
     7141 gttcggtcgt ccactgaaaa gtgagcgcca gagccagcat acctttcagg atcccggttg
     7201 ccgccatcag accgatcaga ggcgtaaaaa tacctgaaat aacataaaca aagcggttta
     7261 gcagattacc tttctcatca ttttccggtg cctgttgcgc tttttcgcca aggcctgcca
     7321 cgctgttaac cgccaggaag acatcggcca catggttacc tatgaccacc tgaaactgac
     7381 caccgctttc caccaccata ataataccgg gggtcttttt cagtacctca gcttgcgctt
     7441 tgctttcatc ctttaactta aaacgtaatc gcgttgcgca atgcatcaga ctcacaatgt
     7501 tatctgcgcc cccgactcct gcgactattt ttctggctaa ctccgtcata acttgccctc
     7561 taccgctttg cggcaaaact ccaaaaaaaa acctgaaaaa aacggcctga cgtgaatcaa
     7621 gcaatttttt tcaggttttg cccgcttagt gcggtaacaa tcctttactc agtaataata
     7681 tttcagtgtt ctttgcgcac gcgctctata tttatggcta aaaacataat ctctgcgggt
     7741 gaaattttac gttgatactg caaaccaata aaaatggcga tccgttccgc acattgccat
     7801 gcttgcgggt aattttgttt tactgcttgt tgtaatgatt catcactatc gttaattgaa
     7861 gcatgttcaa gaatacgcca agataaaaac ttcagatgtg taaccagtcg ctgataactc
     7921 aagctttctt cctggtaatt aaggctgaac tgaaatttta ttaattgcag catttcgcgc
     7981 attaactgcg tgacacctgc aacatcctcc atatttccgc tcatttgggc actgaccaga
     8041 tgcatggcaa taaagcccac ttcatctttc ggtaactgca cgcccaaccg tttatcaata
     8101 atggttaacg cttcttcccc tagctggaac tctttcgggt aaagccgctg gatatcccac
     8161 agcaacacgt tttgctgaaa gcgtttaatc gcaaactggc aatggtcagt tagcgagata
     8221 taaatactgt cctgtaattt tcccaagcgc tcctgcgcta aagagataag acgatcacag
     8281 gttgccatca cctcaagagg aatatgactt aagagttcgc ttaatcgccc gttcagttca
     8341 tgactgctca aggcatactc tttttctatt ccacttgagt taattctttc gccagcgcgt
     8401 ttttgaaagc caattccgcg ccccatgacg actttttccc gctgttgatc atcaataacc
     8461 accacaacat tattgttgag aattttggtg atttgcatgt tcatagcaag gacctttttt
     8521 ataaacaaaa aaaaaccgac ttcaccagta ttctctggtt atgtcaggtt ttgcctgcga
     8581 atgcagtaac aatccagtca tttattaatg gtttttataa cgaacatcca ggttcggaaa
     8641 ttaatttaat tgcgtgcaga gaaaatagca atgcgctatt gataaaaata tgaccatgct
     8701 cgcagttatt aactttatgt aattttagga atttataaag ttatatataa caaatcccaa
     8761 taattaagtt attgggattt gtctggtgaa ttatttgtcg ctatcttttc ccgccagcag
     8821 tttatccagc tcatcaccgc ctacatgacg gaaatcctga cccttcacgt agtagaagat
     8881 aaactcacaa atattctggc agcggtcgcc gatacgttcg attgaacgcg cacagaacag
     8941 cgcggtaagt acgctcggaa tagtgcgcga atcttccatc atgtaggtca tcagttgacg
     9001 aacaatacct tcgtattcct gatcgacttt tttatcttca cgataaatac gtaccgcttc
     9061 gtcaatgtcc atccgcgcga acgcgtccag cacgtcgtgc agcatctgga tggtatgacg
     9121 gcccagcgac tccagactta ccagcaacgg ctgatgctgc tgggagaatt tctccagcgc
     9181 agtacggcag attttgtccg ccacgtcgcc aatacgctcc agctcggcaa tggttttact
     9241 gatcaccata accagtcgca ggtcgctcgc cgtcggctga cgtttggcga taatgcgcac
     9301 gcaggcttca tcgatcgcca cttccatcat gttgacgttc ttgtcgcctt cgatcacgcg
     9361 cttcgccaga tcgctgtcct ggttatgcat cgcggtgatt gcatcagaaa gctgctgctc
     9421 caccatgccg cccatggtca tcacctgcgt gcggatactt tccagttcgg cgttgaactg
     9481 gccggaaata tgtttattaa gattgagact gtccataacg cactcctgaa tcaaccgtaa
     9541 cgaccggtga tgtagtcttc tgtttgtttc ttcgctggct tggtgaacag atcgtccgtg
     9601 ttgctgaact caatcaattc gcccaggtac ataaatgccg tgtggtcgga acaacgcgca
     9661 gcctgctgca tgttgtgggt gacgatcacc acggtgtaat cctgcttcag ttcggtgatc
     9721 agctcttcaa tacgcccggt agagatgggg tcgagcgccg aacacggttc gtcgagcagc
     9781 agcacttccg ggcgaatggc gataccacgc gcaatacaca gacgctgttg ctgaccacca
     9841 gagagagagt aaccgctctg gtgcaattta tctttggttt cgttccacaa tgcggctttg
     9901 gtcaatgccc actgcacgcg ctcgtccatg tcggcacagg agagcttctc aaacagacga
     9961 acgccaaagg cgatgttgtc gtagatggac atcgggaacg gcgtcggttt ctggaacacc
    10021 atgcccactt tcgcacgcag cagggcgata tcctgagagt tggtcaggat gttgtcgcca
    10081 tcaagcagaa tttcaccttc cgcacgctgc tccgggtaca gttcaaacat tttgttgaag
    10141 gtacgcagca gcgtcgattt accgcagccg gacggcccga taaacgccgt aacctggttt
    10201 ttggcgatat ccaggttgat gtttttcagg gcatggaatt tgccgtagta gaagttcaaa
    10261 ttacgaacct gaattttact cggggcagtt tcaaccatac tcattgcaat cttttcctca
    10321 tcgtacctga tgcttttcgc taatcaggct taccgtaggg ccgggtaagg cgttcacgcc
    10381 gcatccggca aagtgttaac cgtgtttatt cttcgcaaaa acaacgcgcg ccagaatgtt
    10441 cagcagcagg acgcacaggg taatgatcaa taccccggcc caggccagtt gttgccattc
    10501 cgcaaacggg ctcatcgcaa acttaaagat cgtcaccggc aggttggcga tcggctgcat
    10561 catgtccgtg ctccagaact ggttggagag cgcggtaaac agcagcggcg cggtttcacc
    10621 ggcaatacgg gcaatcgcca gcaggatacc ggtcataatc ccggacaccg acgctttcag
    10681 cgtaatcgcg gagatcatct tccacttcgg tgtacccagc gcataagccg cttcacgcag
    10741 gctgtccggc accagtttca gcatgttctc agtggtgcgg ataacgatcg gcacctgcaa
    10801 cagcgccagc gcaattacgc ccgcccagcc ggagaagtgc tccatctgtg ccaccacgat
    10861 ggtgtagaca aacagaccaa ccacaatcga cggcgcggag agcagaatgt cgttaatgaa
    10921 gcgaatcact tctgccagcc aggatttacg accatattcc gccagataaa tccccgccat
    10981 aatgcccagc ggcgtaccga ataccgtggc ccacaaaatt aacagcccgc tacccgccag
    11041 ggcgttcgcc agaccaccac cttccgtatt gggcggcggc gtcatttcag tgaacagcgc
    11101 cagcgacata ccgtcgatac cgcgagtgat ggtggacatt aaaatccaga tcagccagaa
    11161 caggccgaag gccatcgtcg ccatcgagag cgtcagcgca atacggtttt tgaggcggcg
    11221 acgcgcctgc attttgcggc gagattcagc cagcgccgca gtggtttgca tttcaaccat
    11281 agccattagc gtgccccctc attcttagcc aggcgcataa tcataaactt cgatgcggcg
    11341 aggacgatga aggtaatcac aaacaggatc aggcccagtt ccatcagtgc ggcaacgtgc
    11401 agaccggatt ccgcttccgc aaattcgttc gccagcgcag aggtgatact attgcccggc
    11461 atgtacagcg aggcgctgtc gagctggtag gtgttaccga tgataaaggt caccgccatg
    11521 gtttcaccga gcgcgcggcc cagccccagc atgatgccgc cgataacacc atttttggtg
    11581 aacggaagaa cgatacgcca gataacttcc caggtggtgc agccaatacc gtaggccgac
    11641 tctttcatca tcaccggggt ttgttcgaac acatcacgca ttaccgccgc aatgtacgga
    11701 ataatcatga tggcgaggat cacgcctgcc gcgaggatac cgataccaaa tgcggggcca
    11761 gagaacagcg cgccaacaat cgggatattc gacatgatat tgccgaccgg ctcctgaaag
    11821 taaacggcga acagcggcgc aaagataaac aggccccaca tgccgtaaac gatacttgga
    11881 atggctgcca gcagctcaat ggcgataccc agcgggcgtt tcagccagcc aggcgcaagc
    11941 tcagtcagga acagggcgat accgaaactc accgggacgg cgatcagcag cgcgataaac
    12001 gaagtcacca acgtaccgta gatcggcacc agcgccccgt agatatcgtt cggtgcatcc
    12061 cactctttgg tccataggaa agccagacca aatttctgaa tgctcggcca ggaggagatg
    12121 atcagagaga caataatgcc acccaacatc aatagcacaa tcagcgccgc cagttttacc
    12181 agcacgctga aaattatgtc gcccttttta cccggtgggt taaaagcggg cttggttgca
    12241 gccataagtt actcttcagt taaacgcgtt tacaaagtca ttttgtaatg ccggatgcgg
    12301 cgtaaaacac cgtacccggc ctggagtttt attagtacag cggcttaccg ctactgtctt
    12361 taatattggt cttccacgca gcgcgaacct gttcaactac actatccggc aggctggcgt
    12421 aatccaggtc gttcgcctgt ttagccccgg ttttgtacgc ccagtcgaag aatttcagca
    12481 cctctgtgcc ttgttctggt ttcttctgat ctttgtggat cagaatgaac gtggtagagg
    12541 taataggcca tgcatcttcg cctttctggt tggtcagatc ctgagcgaag gttttgctcc
    12601 agtctgcacc ttttgctgca ttagcgaagt tttcttcggt cggactaacc ggtttaccat
    12661 cagcggagat cagtttggtg tacgccaggt tgttctgctt cgcgtaagca tattcaacat
    12721 aaccaattgc acccggcaga cgctgaacga acgcggcgat accgtcgtta cctttaccgc
    12781 ccagaccgat cggccatttt acggtagagc cagtaccaac gttgtttttc cactcttcgt
    12841 tcactttcgc caggtagctg gtgaagacga aggaagtccc ggagccatct gcgcggcgta
    12901 ctacagcaat gttttgtgaa ggcagtttca gacccggatt cagtttggcg atggcttcat
    12961 catcccactt cttgattttg cccaggtaga tgtcgccgag ggttttacca tccagcacca
    13021 gttcgccaga cttcagccct ggaatgttaa ccgccagcac cacgccgcca atcacggtcg
    13081 ggaactggaa cagaccttcc tgagccagtt tttcgtcaga cagcggcgcg tcagaggcac
    13141 caaaatcaac ggtattagcg ataatctgtt ttacgccacc ggaagaaccg ataccctggt
    13201 agttaacttt attaccggtt tctttctggt aagtgtcagc ccatttggca tacaccggcg
    13261 cagggaaggt tgcacctgca cctgtcaggc ttgcttctgc aaacacagag aaagcactca
    13321 tcgataaggt cgcggcgaca acagttgcga cggtggtacg cataactttc ataatgtctc
    13381 ctgggaggat tcataaagca ttgtttgttg gctacgagaa gcaaaatagg acaaacaggt
    13441 gacagttata tataaggaat atgacatttt tatgacagaa aggaattacc aaataactta
    13501 ctgattttaa atgtgaagat gaaaatccca catgccatct ttttgtcatg atgcatttaa
    13561 aagtgataaa gaaatgggtg agatgacaac gatgtgatga acaatcgttg tcagacctat
    13621 tagcgcacaa tctcaacagt tatcgtcgct gttgcagtaa attgccccgg ggctgggcga
    13681 gctccagtaa ggctcgcagg agcagctgaa aaagttacac tacctgattt ttcaccatac
    13741 actgttgatt tatccatgtc tgttggcaac tcaccaccgt tcactttgat ttccttatta
    13801 tttttgtcgt aaatttttat ccccacgtta gagtttgaag ttacgaccat tgaattgtcc
    13861 gtattgggtt cggtagcata aaaagacgta ctgatatcat cggtattcag tatccccagg
    13921 ttttcacact caatagtgac tgttttagta attttatgtg ttgttaccgc acttccaaca
    13981 acagatgaaa aatctgcagc tggtatttct ttaaaatcaa aacaaactgg ctttaaatta
    14041 tttatttcgc aactttgcgg agctgtaata tcgccccgaa tataaacatc agccatcggc
    14101 tgtgagtaat ccaccggggt gtcacggcta attgttccat acaatgtcac tatctttgtt
    14161 tcaggaataa tcaattttcc tgcaaatgtc ttcttgatat aaaaatatac tatcgcttta
    14221 gccccactgg atatacttaa aggttcaatg cggttgcaat tgtactttgt attaccagac
    14281 gggttgttag catgatattc aaaaggtgca ttcacatacc caaccccaag aatatctata
    14341 gcaatcccaa catcaaggtt cggattaaga tcataataat taatattaga gtgaacccca
    14401 gcataagtta atgcctggtt tatttttgac gtataataga tataggggaa ttctcctacg
    14461 ttaccagtat cacaatgaca gcgtccaaga tatgaggatc cattagttac agtggtgtta
    14521 aatgtcgttc caaccttatt ttcgttcgcg gtaataagat atccc
//
LOCUS       DAJTQY010000093        14513 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.093, whole genome shotgun sequence.
ACCESSION   DAJTQY010000093 DAJTQY010000000
VERSION     DAJTQY010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14513
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.093"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(111..377)
                     /locus_tag="OO349_003088"
     CDS             complement(111..377)
                     /locus_tag="OO349_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HCR5868078.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPSLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            651..4175
                     /gene="nifJ"
                     /locus_tag="OO349_003089"
     CDS             651..4175
                     /gene="nifJ"
                     /locus_tag="OO349_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HCR5868079.1"
                     /translation="MITIDGNGAVASVTFRISEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFAAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPSG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRQEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACPGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAANLQKRYDFLAQMAGKAEKSNTD"
     gene            4542..5671
                     /gene="ompN"
                     /locus_tag="OO349_003090"
                     /pseudo
     CDS             4542..5671
                     /gene="ompN"
                     /locus_tag="OO349_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
     gene            5812..6246
                     /gene="uspF"
                     /locus_tag="OO349_003091"
     CDS             5812..6246
                     /gene="uspF"
                     /locus_tag="OO349_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HCR5868080.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            6422..7357
                     /gene="ttcA"
                     /locus_tag="OO349_003092"
     CDS             6422..7357
                     /gene="ttcA"
                     /locus_tag="OO349_003092"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HCR5868081.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGTTKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(7486..8853)
                     /gene="dbpA"
                     /locus_tag="OO349_003093"
     CDS             complement(7486..8853)
                     /gene="dbpA"
                     /locus_tag="OO349_003093"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HCR5868082.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVEQSALSLHGDLE
                     QRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTARAG
                     NSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVTLEAEMATLCIDGGKKAK
                     MRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKTCR
                     VRLLK"
     gene            complement(9331..10314)
                     /gene="zntB"
                     /locus_tag="OO349_003094"
     CDS             complement(9331..10314)
                     /gene="zntB"
                     /locus_tag="OO349_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HCR5868083.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRFGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            10569..11801
                     /gene="dgcM"
                     /locus_tag="OO349_003095"
                     /pseudo
     CDS             10569..11801
                     /gene="dgcM"
                     /locus_tag="OO349_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
     gene            complement(11822..12385)
                     /gene="smrA"
                     /locus_tag="OO349_003096"
     CDS             complement(11822..12385)
                     /gene="smrA"
                     /locus_tag="OO349_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HCR5868084.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRQEGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMV
                     FSFIQQALADGLRNVLIIHGKGREDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(12639..14093)
                     /locus_tag="OO349_003097"
     CDS             complement(12639..14093)
                     /locus_tag="OO349_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HCR5868085.1"
                     /translation="MMSMRKHIAFASMCMGLFIAQLDIQIVSSSLNEIGGGLSAGKDE
                     MAWLQTSYLIAEIIVIPLSGWLSRVFSTRWLFTLSAGIFTLMSIACGLAWNIQIMILF
                     RALQGAAGASMIPLVFTMAFIYYQGKELGLAAAVVSALASLSPTLGPTLGGWLTDNLD
                     WRWLFYINILPGIYLVLSIPFLVNFDKPDLSLLKVADYPSIILLAMTLGCLEYTLEEG
                     ARWGWLDDNTILLTSVLALVSFILFAARTLTISNPIMDLHAFKDKNFTLGCFFSFSGG
                     VGIFSTVYLIPVFLGQIRGLNAEEIGFAVCTTGIFQLFSVPFYFWLSKKINLRWLLMA
                     GLGGFVFSMYLFTPITHEWGWQELLFPQAIRGISQQFAMAPIVTLTLGGIPKERLKLA
                     SGVFNLTRNLGGAIGIALCGSILNNRTNFHFSRMGEKMVSVPHTVNDFISRSALFFNR
                     SRSDQTSEILASTKLLSQLMLREAQTMAFSVPFC"
     gene            complement(14090..>14513)
                     /locus_tag="OO349_003098"
     CDS             complement(14090..>14513)
                     /locus_tag="OO349_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR5868086.1"
                     /translation="FSICSRQAAVVRAPIDGIVANRSAHTGSWVEGGTSLVSLVPVSE
                     LWVDANYKENQIAGMKPGMKAEIRADILKGEVFHGHIESLSPATGASFSLIPIENATG
                     NFTKIVQRVPVRIAFDDAKELKQLLRPGLSVTVSVDER"
BASE COUNT         3871 a         3661 c         3430 g         3551 t
ORIGIN      
        1 accattagcg aaatgctgaa agaaggtgca caagtggatc tgaccgcgaa ccagttaacg
       61 ctggcgaccg cgaaacagac attaacttat aagctggcgg atttaatgaa ttaatagctg
      121 ccacagctcc cggcggcaag tgactgttcg ctacagcgtt tgccgttggg taatgcacac
      181 atcccaatcg ccgtaccatc cagttgacgg gcaacagaaa gcgaaccgcc gatcattgca
      241 caatttgctt ctccactact ggacatcgac gcttttaaac ttggcgctac gtgcgccgca
      301 gtcgcctgct gaacaggctc actactacac gccgacaaca ataaagcggc acaccctacc
      361 caaaacgctg ctcgcatctc ttcttcctct gatcttcaag ccaaacgaca ccgccataaa
      421 taataggcag cacagagggc ggcgtcgaga gctgtcctgc gcgttgcccc gcatttttac
      481 ttttttatgg ctattttttt gccctctgtt tgatcaaaac attcattact ctgatgtggg
      541 ggacacaaaa gcgaaaatgc agaagaaagc catttgctaa aattgaagga ttactactgg
      601 gcgcgcagca atttcgtgcg cccctcattt gcgcaatgta agggtgtcat atgattacta
      661 ttgacggtaa tggcgcggtt gcttcggtga cgtttcgcat cagtgaagtt atcgccatct
      721 accctattac ccccagttcc acgatggcag aacaggctga tgcctgggcc ggaaacggct
      781 tgaagaacgt ttggggagac acaccacgcg tggttgaaat gcagtcggaa gcgggtgcta
      841 tcgctaccgt gcatggcgct ttgcagacgg gtgccctttc aacatcgttt acgtcatcgc
      901 agggtttgct gctgatgatc ccgacgctgt acaaactggc aggcgaactg acgccatttg
      961 tcctgcatgt agcggcacgt accgttgcca cacatgcact ttctattttt ggcgatcatt
     1021 ccgacgttat ggcggtgcgc cagacgggtt gcgcgatgtt gtgtgcggct aacgtccagg
     1081 aagcgcaaga ctttgctctc atttcgcaca tcgcaacgct gaagagccgc gtgccattta
     1141 ttcatttctt tgatggtttt cgcacgtccc acgaaatcaa taaaattgtc ccgctggccg
     1201 atgacactat tcttgatctc atgccgcagg ccgaaattga tgctcatcgc gcccgggcgc
     1261 tcaacccgga acatccggtg atccgcggta cgtccgccaa tcctgacact tatttccagt
     1321 ctcgcgaagc caccaatccc tggtacaacg cggtctatga ccatgttgaa caggcgatga
     1381 atgatttcgc tgccgcgaca ggtcgtcagt atcagccgtt tgagtattac gggcatccgc
     1441 aagcggaacg ggtgattatc ctgatgggct ctgccattgg cacctgtgaa gaagtggttg
     1501 atgaattgct aacccgtggc gaaaaagttg gcgtgctgaa agttcgtctg taccgtccct
     1561 tctccgccaa acacttgctg caagctctgc cgggatccgt acgcagcgtg gcggtactgg
     1621 acagaaccaa agaacccggt gcccaggcag aaccgctcta tctggatgtg atgaccgcac
     1681 tggcagaagc ctttaataat ggtgagcgcg aaaccctgcc ccgtgtcatt ggtgggcgct
     1741 atggtctttc atccaaagaa tttggcccgg actgtgtact ggcggtattt gccgagctca
     1801 acgcggctaa acctaaagca cgctttacgg ttggtattta cgatgatgtg accaatctgt
     1861 cactgccgtt gccggaaaac actctgccaa actcggcgaa acttgaagcc ttgttttatg
     1921 gccttggtag tgatggcagc gtttccgcaa ccaaaaacaa tatcaagatt atcggtaatt
     1981 ccacgccgtg gtacgcacag ggctattttg tttacgactc caaaaaagcg ggcggcctga
     2041 cggtttctca ccttcgcgtg agcgaacagc cgattcgttc cgcttatctc atttcccagg
     2101 ctgattttgt tggctgccac cagttgcagt ttatcgataa atatcagatg gctgagcgtt
     2161 taaaacctag cggcattttc ctgcttaata cgccgtacag cgcagatgaa gtgtggtcgc
     2221 gcttgccgca ggaagttcag gccgtgttaa accagaaaaa agcgcgcttc tatgtgatta
     2281 acgcggcgaa aatcgcccgc gaatgtggcc tggcggcccg tattaatacc gtcatgcaga
     2341 tggctttttt ccatctgacg caaattctgc ctggcgatag cgccctcgca gaattgcagg
     2401 gtgcgattgc caaaagttac agtagcaaag gtcaggatct ggtggagcgc aactggcagg
     2461 ctctggcgct ggcgcgtgaa tccgtagaag aagttccgtt gcaaccggta aatccgcaca
     2521 gcgccaatcg accgccagtg gtttccgatg ccgcccctga tttcgtgaaa accgtaaccg
     2581 cggcgatgct cgccgggctt ggtgatgccc tccccgtttc ggcgctgccg ccagacggca
     2641 cctggccgat gggcaccact cgctgggaaa aacgcaatat cgccgaagag atccccatct
     2701 ggaaagagga actctgtacc caatgtaacc actgcgtcgc cgcttgccca cactcagcta
     2761 ttcgcgcaaa agtggtaccg cctgaagcga tggaaaacgc ccctgccagc ctgcattcgc
     2821 tagatgtgaa atcgcgtgat atgcgcgggc agaaatatgt cttgcaggtg gcaccggaag
     2881 attgcaccgg ttgtaacctg tgcgtcgaag tttgcccggc gaaagaccgt cagaatccag
     2941 agattaaagc catcaatatg atgtctcgcc aggaacatgt cgaagaagag aaaatcaatt
     3001 acgatttctt cctcaacctg ccagaaatcg accgtagcaa actggaacgt attgatattc
     3061 gtacatcgca gctgattaca ccgctgtttg aatattcagg tgcttgcccc ggttgtggcg
     3121 agacgccgta tattaaattg ctgactcagc tctatggtga ccggatgttg atcgctaacg
     3181 ccaccggctg ttcttcaatt tatggcggta acctgccctc tacaccgtat accaccgatg
     3241 ccaacggtcg tgggccggca tgggcgaact ctctatttga agataatgcc gaatttggcc
     3301 ttggtttccg cctgacggtc gatcaacacc gtgtccgcgt gctgcgtctg ctggatcaat
     3361 ttgccgataa aatcccggcg gaattactga cggcgttgaa atcagacgct acgccagagg
     3421 ttcgtcgaga acaggtcgca gctttacgcc agcaactcaa cgatgttgcc gaagcacatg
     3481 aactgctgcg tgacgcagat gcactggtgg aaaaatcaat ctggctgatt ggtggcgacg
     3541 gctgggctta cgatatcggc tttggcggtc tggatcatgt attgagtttg acggaaaacg
     3601 tcaacattct ggtgctggat acgcaatgct attccaacac cggtggtcag gcgtcgaaag
     3661 cgacaccgct gggtgcagta actaagttcg gcgagcacgg caaacgtaaa gcgcgtaaag
     3721 atcttggcgt cagtatgatg atgtacggtc atgtttatgt ggcgcagatt tctctcggcg
     3781 cgcagctgaa ccagacggtg aaagcgattc aggaagcgga agcgtatccg ggaccatcgc
     3841 tgatcattgc ttatagcccg tgtgaagagc atggttacga tctggctctc agccacgacc
     3901 agatgcgcca actcactgct accggcttct ggccgctata tcgctttgat ccgcgtcgtg
     3961 ccgatgaagg caaactgccg ctggcgctgg attcacgccc gccgtcagaa gcactggaag
     4021 aaacgttact tcacgagcag cgtttccgtc gtctgaattc gcagcagcca gaagtggcag
     4081 aacagttatg gaaagatgct gcagctaatt tgcaaaaacg ctatgacttc ctagcacaaa
     4141 tggccggaaa agcggaaaaa agcaacaccg attaaatgct ctggataagg attatccaat
     4201 tctaaaaaaa aagcccggac gactgttcgg gcttgtcttt ttatatgttg gaaaatcagt
     4261 ggcaatgcaa tggcccaaca gaaaataaaa tctgtgacaa taaaggcata taacccgcgc
     4321 agaataacgt ataggaaatt aaaagtataa tttttatttt gtatgtatta tcttcgatac
     4381 attcaccaga cttattatca tttcagagga attatttgat taaggtttta cttaaggcgt
     4441 aacaaatgat atttatcggc taactgaact tctcctttag gatgttttca ctccccgaaa
     4501 tggggataaa aggcaaataa aataactaaa ggatttattc aatgaaaaga aaagtactgg
     4561 cacttttaat tcctgccctg ctcgccgcag gtgctgcaca tgcagccgaa gtttataata
     4621 aagacggcaa caaattagat ctgtatggca aagttgatgg cctgcattat ttttctgata
     4681 attcagcgaa agatggcgac cagagctatg cgcgtctggg ttttaaaggc gaaacccaaa
     4741 ttaacgatca acttactggc tacggtcaat gggaatacaa tattcaggca aacaacactg
     4801 aatcttcaaa aaaccagtca tggacccgtc tggcatttgc cggactgaaa tttgcagatt
     4861 acggttcttt cgattacgga cgtaattatg gcgtaatgta cgacatcgaa ggctggaccg
     4921 atatgctgcc tgaatttggc ggtgactctt ataccaatgc agacaacttt atgactggtc
     4981 gagccaatgg cgtcgcgact tatcgtaata ctgatttctt cggtctggta aatggtctga
     5041 acttcgcggt gcagtatcaa ggtaacaacg aaggagccag taatggtcag gaaggcacca
     5101 ataacgaacg cgatgttcgt catgaaaacg gtgacggctg gggtctttcc acaacatatg
     5161 atttaggcat gggctttagc gctggtgcgg catacacctc ttctgaccgc accaatgacc
     5221 aggttaacca tactgcggcg ggtggtgata aagcagacgc gtggactgct gggctaaaat
     5281 acgatgctaa caatatttac ctagcaacca tgtattcaga aacgcgtaat atgaccccgt
     5341 ttggcgacag cgattatgca gttgcgaaca aaacccagaa ctttgaagtt actagtacca
     5401 gtttgatttt ggtctgcgtc cggcagtctc tttcctgatg tctaaaggcc gtgaccttca
     5461 cgctgctggt ggtgcagaca acccggcagg tgttgatgat aaagatctgg ttaaatacgc
     5521 cgatgttggc gcgacttact atttcaataa aaacatgtcc acctatgttg actataaaat
     5581 caacctgttg gatgaagatg acagtttcta cgctgccaac ggcatctcta ccgatgatat
     5641 tgtcgcttta ggtctggttt atcagttcta aatcctcctg cccgctgtta tggcgggctt
     5701 tttctgctta ttcttcctcc tttgatctaa attaaaaatg tgaactccgt cattacacaa
     5761 aaagtgtcat ctggcgttac actttatgcg gatactaaaa caggaggttt tatgaacaga
     5821 acgattcttg tccctatcga tatttcagat tcagaattaa ctcaacgcgt gattagccac
     5881 gttgaggcag aggcaaagat tgatgatgca gaggttcatt tcctgacggt aataccttca
     5941 ctgccctact atgcctctct gggtttagcg tattccgcag aattaccggc aatggatgac
     6001 ctgaaagcgg aagccaaatc gcaactggaa gagatcatta aaaaatttaa actgccaacc
     6061 gacagagtgc atgtccatgt tgaggaaggc tcgcccaaag accgcattct ggaattggcg
     6121 aagaagatcc ccgctcatat gatcatcatt gcttcccatc gaccggatat caccacttat
     6181 ctgctcggtt ccaacgccgc agctgtagtg cgtcacgcag agtgctccgt gctggttgtg
     6241 cgctgacact aacgcccgca cattgctgcg ggctttttga ttcatttcgc aaatgtgctg
     6301 acattttccc ctctaatccg taccatacac gccacagttt ttatatcaga tttcttatgc
     6361 tgagcgttcc ggcatgacgc atactcttct gatgccatat aacgaattga gtcgctttta
     6421 aatgtcgcaa aatcaagaaa ttagtaagaa agaacaatac aacctgaaca aattacaaaa
     6481 acgtctgcgt cgtaacgtgg gcgaagccat tgctgacttc aatatgattg aagaaggcga
     6541 tcgcatcatg gtttgcctct ccgggggtaa agacagctat accatgctgg agattctgcg
     6601 caatttgcag caaagcgcgc caatcaattt ttcgctggtg gctgttaacc tcgatcaaaa
     6661 gcaaccgggc ttcccggaac acgttctgcc cgagtatctt gaaaagctgg gcgttgagta
     6721 caagattgtt gaagagaaca cttacggtat cgtgaaagag aagattccag agggcaaaac
     6781 cacttgctca ctgtgttctc ggctacgtcg cggtatcctt tatcgcaccg caacggaact
     6841 ggggacgacg aagatcgcgt tgggtcacca tcgtgacgat atcctgcaaa cgttgttctt
     6901 aaatatgttc tacggcggca agatgaaagg tatgcctccg aaactgatga gcgatgatgg
     6961 caaacatatc gttattcgtc cactggccta ctgccgcgag aaagatattc agcgatttgc
     7021 cgatgcaaaa gcgttcccga ttattccgtg caacctgtgc ggttcacagc ctaacctgca
     7081 acgtcaggtg attgctgaca tgttgcgtga ctgggataaa cgttatccag ggcgtatcga
     7141 gacgatgttc agcgcgatgc agaatgtggt gccgtcgcat ctgtgcgata ccaacctgtt
     7201 cgatttcaaa ggcattaccc acggttctga agtggttaac gggggtgatc tggcgtttga
     7261 tcgcgaagag atcccactac aaccggcggg ctggcagcca gaagaagatg aaaatcagtt
     7321 ggatgagtta cggctgaatg tggttgaagt gaaataacca agatagcgcc cgatgcgcaa
     7381 gcgtatcggg ctactcttat ggaggccgga taagacgcgg ccagcgtcgc atccggcaat
     7441 cccgaataag atgtttactc ttgcacccgg caattcaaca tttcattatt ttaataaccg
     7501 cacccggcac gtttttcctt taatcttccc gccctgtaac tgtttccatg ctttatgagc
     7561 aacagcctga cggaccgcga catagacatg cgccggatgc acggcgattt tgccaatatc
     7621 tgcgccatca agcccgatat ctcctgtcag tgcacctaat acatcacccg ggcgcatttt
     7681 ggcttttttc ccgccatcga tacacaacgt tgccatttct gcttccagcg tcacaatgga
     7741 actattagct ggcggcgttt gccagttaag ttttatctgc aacatgtcag aaatgatatt
     7801 ggcccgctgt gcttcttccg gagcacagaa actgatcgcc agaccgctat ttcctgcacg
     7861 agctgtacga ccgatgcgat gtacatgaac ttcagggtcc cacgccagct caaagttcac
     7921 caccagctca agcgatttaa tatccagacc acgcgcagca acatcagtcg caaccagtac
     7981 acgggcgcta ccgttagcaa aacgtaccag ggtctgatcg cgttgctcca gatcgccgtg
     8041 taacgacaat gcactttgct ctacttcatt cagcgcgtcg cagacagcct ggcaatcttt
     8101 tttggtattg caaaacacca cacaagagga tggctgatgc aagcttaata accgttgcaa
     8161 cagaggaatt ttgcctttac tggatgtctc ataaaattgt tgttcaatgg gtggcaaagc
     8221 atctgttgag tcaatttcaa tcgccaaagg atcgcgttgc actcgtccgc tgattgcggc
     8281 gatggcttcc ggccaggttg ccgaaaacag aagcgtctgt cgagatgcag gcgcaaaacg
     8341 gatgacatca tcaatggcat cgctaaatcc catatccagc atgcggtcgg cctcatccat
     8401 caccaaagta ttcagcgcat ccagtgatac cgtgcctttt tgcaagtgat ccagcaaacg
     8461 acccggcgtt gccacgataa tatgcggcgc atgttgcagc gaatcacgct gcataccgaa
     8521 cggttgacca ccgcacaacg tcaaaatttt ggtatttggc agaaaacgcg ccagccgacg
     8581 caattcacct gccacctgat ccgccagttc acgcgtagga cacagcacta aagcctgggt
     8641 ttgaaatagc gacgcatcaa tttgctgtaa caagccgagg ccaaaagccg ccgttttgcc
     8701 gctgccggtt ttcgcctgca cgcgaacatc ttttccggca aggatcgccg gaagcgcggc
     8761 ggcctgcacc ggcgtcatgg ttaaataacc caactcatta aggttcgtga gttgggcggg
     8821 aggcaaaaca ttcagggtag aaaaagcggt cacaatctat tctcgtggtc atcgacgcaa
     8881 agttagcagg cgcgtatcct cgcagatcta cgctcacgat gcgacaattt aatcggttct
     8941 tcatcgggtg gtgggtcagg catgggttgc gggcgaggga tcggatcggg cactggaaca
     9001 ggatcgccag gaatcggttc agggacagga atttgcaaat aaataagtgt cgtcatattt
     9061 ccctccggtc attgggtgga ctcttaaagg gtagacgctg ataaataaca ggcaaaaaaa
     9121 agccgactca tcaaagtcgg cgtcgtacga atcaattgtg ctatgcagta attcaaaaaa
     9181 ggaagtaaga caatatggag cgcaacgccc atcgcttgac gttgcattca cctgcaaggg
     9241 agatattgcc ctgaatgggt agagagttta ttgacttcgc tcaaactttg cggcgttttt
     9301 gtatacagac agccggaaaa attgcttttg ttacaaccat ttactacggt gcaaccataa
     9361 agcaacacca ccaataagaa caactaacag aatacaaaaa attgaaaatc cgaattgcca
     9421 cccgccgcca gggatcccac caaggttgac gccaaataac ccggtcagaa aggtactggg
     9481 taaaaagacc attgccatca acgacattgt ataggtacga cgagctaaat tttcctgcat
     9541 cacctgagcg atttcatccg ccatcacgcc agtccgtgct atacaggcgt cgatttcgtc
     9601 aaggccgcgc ccaaggcgat cggcaatatc ctgcatccga cggcgttggt catcgctcat
     9661 ccacggcaaa cgttcactgg caagacgagc ataaacatca cgttgcggtg ccatatagcg
     9721 acgcatcaca attaattgtt tgcgcagcag agccaggaat ccacgcggtg gaatttgctg
     9781 atcaaggaga ttatcttcaa ggtcgataat tttatcgtgc agctgctcga taaattcact
     9841 ggaatgatcg gtcaacgcat cgcacacatc caccagccat cccccgcaat cggtcggacc
     9901 cgtgccctct tccagatcgc tcaccacatc gtccagcgcc agcactttgc gttgtcgggt
     9961 cgaaacaatt aaccgcccgt ccatatatac acgcatggcg accagttgat cggggcgttc
    10021 atcggtgctg ccgtttatac agcgcaatgt aatcagcgtg ccttcaccga aacggctgac
    10081 tcggggccgt gtgctctcgc ccgccagtgc atcacgtacg ttattgggaa gcagcggtgt
    10141 tgtcgccagc cattgggcgc tatcatggtg tacataatta aggtggagcc agcagggatg
    10201 cgcttcatca atcacatctg tattttccag cggtttaacg ccgcctctac catccagcat
    10261 ccaggcaaat actgcatccg ggacattaac gtccgatccc ttaatcgctt ccacagtgcc
    10321 tccatcatca acgcattatt ttgtagtcta gccttctggc cctgttacgc aacatctcat
    10381 caccccatta ccctgaaatg attaataaaa ttctgtctaa attgaataca aaaagcaaaa
    10441 tgcttttccg tatacaaacc gtgtgaagtg ttaaatagcg tctatcatta tcagaattat
    10501 ctgatcatat gacgtggctt ttttgcgatc ggatagcaac aaaaattgat aaaaataacg
    10561 ggatctcaat gattacgcac aacttcaata ccctggactt actcaccagt cctgtctgga
    10621 ttgtttcgcc ctttgaggaa cagttaattt atgccaatag cgcggcgcga ctgttgatgc
    10681 aagacctcac gtttagtcag ctacgaaccg gaccctattc cgtctcctca caaaaagaac
    10741 tgccgaaata cctctccgat ctgcaaaacc aacacgatat tatcgaaatc ctcactgttc
    10801 agcgtaaaga agaggaaaca gcattaagct gtcggcttgt tttgcgagag ctgacagaaa
    10861 cagaaccggt gattattttc gaaggtatcg aagcgccggc aacgctgggt ttaaaagcca
    10921 gtcgctcggc aaattatcag cgcaaaaaac aaggttttta tgcgcgcttt tttctgacta
    10981 actctgcacc aatgttgttg attgacccgt cacgagatgg acaaatcgtc gatgctaacc
    11041 tcgccgcgct caatttctat ggttataacc atgaaacgat gtgccagaaa catacctagg
    11101 aaataaatat gcttgggcgt cgcgtcatgc ctatcatgca tgaaatttcg catttacccg
    11161 gcggtcataa ccctttgaat tttgttcata aactggcgga tggatctact cgtcatgtgc
    11221 agacctatgc cggaccgatt gaaatttatg gcgacaagct catgttatgt attgtgcatg
    11281 atattactga gcaaaaacgg ctggaggagc agctggaaca tgctgctcac catgacgcga
    11341 tgaccggatt actgaatcgg cgacagtttt atcacattac ggaaccaggc caaatgcagc
    11401 atctcgccat cgctcaggat tacagcttgt tgctcatcga caccgatcgt tttaagcaca
    11461 ttaacgatct ctatgggcat tctaaaggtg atgaggtgtt atgcgccctc gcccgccccc
    11521 tcgaaagttg cgctcgcaaa ggcgatttgg tgtttcgttg gggaggcgaa gagtttgtct
    11581 tattgctacc aagaacccca ctggataccg cgctttcgct ggctgaaact atccgcgtaa
    11641 gcgtggcaaa agtgagtatt tcgggcttac cacgctttac cgtcagcatt ggtgtggcgc
    11701 atcacgaagg aaatgaaagc atcgatgaac tgtttaaacg cgttgatgat gctttgtatc
    11761 gggcgaaaaa tgatggacgc aaccgcgtgc tggcggcata agccgcggat gcgtctcgag
    11821 atcaacgact gcgcttagcg tggcgctccc agttttcttg cttcgcctgc gccgttttac
    11881 gtagtgcgac gtaacacgcc ccgctgccgc catgatgcgg tagcgcggtg caatatgcct
    11941 gaacatcatc aaattcggtc agccagcgcg ccacatagct gcggacaata ttggcatgtg
    12001 atttatcttc ccgcccttta ccatgaataa tcagcacatt acgcaaacca tccgccaggg
    12061 cttgttgaat gaaactgaac accattttgc ggcactcttc caccggctgg cgcaaaagat
    12121 tcaggctcgc ctgttgcgga tatttaccac tgcgcagctt atccagcacc ccatgctgca
    12181 acccttcctg ccgaaactcc agcggctgac ttagcgggat gatgtcgaga aatccggtag
    12241 tgaggaaatt atcaagctgc agcgtgtcga tacgctgcgg cgcacgttgg ttacgcgttg
    12301 gatgccagtg gacatctgta gcacgtttca gcggctggac atcttccatg gcgtcaagaa
    12361 acagcgattt gtcgtcaagg ttcatgtaac ctcctatgca ccttcacacg tcgtgattca
    12421 gacgggttaa ttgttcatac ttactgcatc gttcccttca cagattgaaa caatactcta
    12481 cttcatacct gtgagtatct gcgcaggcgg ggcattttat actagcctgt tagcagtatc
    12541 gaacagttta gcccgagttt aattcagcac aggctccgat caggatgatt tattcatagc
    12601 aggaaccaac aaaaatgcga taaataacag accactgatt agcagaaagg taccgaaaat
    12661 gccatagttt gcgcctccct cagcatcaat tgactcaata atttggttga agccagtatt
    12721 tccgatgtct ggtcacttct actgcgatta aaaaataaag cacttctgga aatgaagtca
    12781 ttcaccgtat ggggaacaga aaccattttt tcacccattc tcgaaaaatg aaaattagtt
    12841 cgattattga gaatagagcc acacagggca ataccaatcg cgcccccaag attacgtgtt
    12901 aaattaaata caccagaggc taacttcagc cgttcttttg gaattccccc cagcgttaat
    12961 gttacgatgg gggccatcgc aaactgctgc gaaatccctc taattgcctg tggaaacagt
    13021 aattcctgcc atccccattc gtgggttata ggggtaaaaa gatacattga aaacacaaat
    13081 ccgcccagcc ccgccatcaa cagccatcgc agatttattt ttttacttaa ccagaagtaa
    13141 aagggaacag aaaataactg gaaaatacct gttgtgcaaa cggcaaaacc tatctcttcg
    13201 gcatttaatc cgcgaatctg ccccaaaaac acaggtatta aatatacagt agagaatatc
    13261 ccaacccctc cagaaaaaga aaaaaaacat cccagagtga aatttttatc cttaaaagca
    13321 tgaagatcca ttatcggatt ggatattgta agtgtacgcg cagcaaataa aataaaactc
    13381 accaatgcca gaactgatgt cagcaaaata gtattatcat ccagccatcc ccagcgcgct
    13441 ccttcttcaa gggtatattc cagacatccc agagtcatgg ccaacaggat aatactcgga
    13501 taatcagcaa ctttcagtaa agagagatca ggtttatcaa aattaaccag aaaaggaata
    13561 ctcaaaacta aatatatgcc aggtaatata ttgatataaa atagccatcg ccagtcgagg
    13621 ttatcagtga gccagccccc cagagttggc cctagcgtcg gtgacagtga ggccagcgca
    13681 ctgactaccg cagcagccaa ccccaactct ttaccctggt aataaataaa cgccatcgtg
    13741 aaaactaaag ggatcatgga tgcgccagca gctccctgta aagcacgaaa caaaatcatg
    13801 atctgaatat tccatgcaag tccacacgcg atactcatta aagtaaatat tcccgcggat
    13861 agagtaaaaa gccaacgagt ggaaaatacc cgtgacagcc acccagagag cggaataaca
    13921 ataatctcag caattaaata gctggtctga agccaggcca tttcgtcttt acctgcggac
    13981 agaccaccac ctatttcatt tagggatgac gatacaattt gaatatcaag ctgagcaata
    14041 aataatccca tacacatact ggcgaaagca atatgtttac gcatagacat catctctcat
    14101 caacactgac ggtgacggac agtcccgggc gaagaagttg ctttaattcc tttgcgtcgt
    14161 caaatgcaat ccgtaccggg acacgctgaa caattttggt gaaattgcca gtggcatttt
    14221 ctatcggaat caagctgaaa ctggctcctg tcgctgggga aaggctttca atgtgaccgt
    14281 gaaacacttc ccctttgaga atatcagcac ggatctcagc cttcattcct ggcttcattc
    14341 ccgctatttg gttttcttta taatttgcat caacccacaa ctcactgact ggtactagtg
    14401 aaactaacga tgtgccgcct tccacccaac tgcctgtatg ggcgctgcgg tttgcgacaa
    14461 tgccatcaat cggcgcacga accacggcag cctgacggct gcaaattgaa aac
//
LOCUS       DAJTQY010000094        14475 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.094, whole genome shotgun sequence.
ACCESSION   DAJTQY010000094 DAJTQY010000000
VERSION     DAJTQY010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14475
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.094"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(337..582)
                     /locus_tag="OO349_003099"
     CDS             complement(337..582)
                     /locus_tag="OO349_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5868087.1"
                     /translation="MKYKEVACGKQRMHWLALQFVDENNNPVSGLNVQLEYHPRAMAA
                     ELALWARGRHTQFDPTPPPPPVGVTDSQGLVRFDDLY"
     gene            complement(1227..1302)
                     /locus_tag="OO349_003100"
     tRNA            complement(1227..1302)
                     /locus_tag="OO349_003100"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1267..1269),aa:Phe,seq:gaa)
     gene            complement(1408..2115)
                     /locus_tag="OO349_003101"
     CDS             complement(1408..2115)
                     /locus_tag="OO349_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HCR5868088.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLITPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            2513..4648
                     /gene="speC"
                     /locus_tag="OO349_003102"
     CDS             2513..4648
                     /gene="speC"
                     /locus_tag="OO349_003102"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HCR5868089.1"
                     /translation="MKSMNIAASSEVVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGTTPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LTIIQLGTYDGTVYNARQVIDTIGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQLVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSSLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(4697..5953)
                     /gene="nupG"
                     /locus_tag="OO349_003103"
     CDS             complement(4697..5953)
                     /gene="nupG"
                     /locus_tag="OO349_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HCR5868090.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(6155..7234)
                     /gene="mltC"
                     /locus_tag="OO349_003104"
     CDS             complement(6155..7234)
                     /gene="mltC"
                     /locus_tag="OO349_003104"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HCR5868091.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQNTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(7299..7574)
                     /locus_tag="OO349_003105"
     CDS             complement(7299..7574)
                     /locus_tag="OO349_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HCR5868092.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(7602..8654)
                     /gene="mutY"
                     /locus_tag="OO349_003106"
     CDS             complement(7602..8654)
                     /gene="mutY"
                     /locus_tag="OO349_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HCR5868093.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMAHFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAATNNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRHWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            8815..9534
                     /gene="trmB"
                     /locus_tag="OO349_003107"
     CDS             8815..9534
                     /gene="trmB"
                     /locus_tag="OO349_003107"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HCR5868094.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            9534..9860
                     /locus_tag="OO349_003108"
     CDS             9534..9860
                     /locus_tag="OO349_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HCR5868095.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKIVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEECKLDEVRTSEL
                     FDVWWD"
     gene            10044..10763
                     /locus_tag="OO349_003109"
     CDS             10044..10763
                     /locus_tag="OO349_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HCR5868096.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGDSSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            10939..11985
                     /gene="ansB"
                     /locus_tag="OO349_003110"
     CDS             10939..11985
                     /gene="ansB"
                     /locus_tag="OO349_003110"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HCR5868097.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINADCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            12102..13063
                     /locus_tag="OO349_003111"
                     /pseudo
     CDS             12102..13063
                     /locus_tag="OO349_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708727.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
     gene            complement(13164..>14475)
                     /locus_tag="OO349_003112"
     CDS             complement(13164..>14475)
                     /locus_tag="OO349_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HCR5868098.1"
                     /translation="DSYIAITLFGCFFVLVFIGVPISFSIGIATVASMLLMFPWDIAA
                     ITVSQRLANGLDNFALLAIPFFIFAGTLMNSGGIAIRLINLAQVMVGRVPGSLGHVNV
                     LANMMFGSISGSAVAAAAAVGGTLNPIQTKEGYDPAFSTAVNVSSCITGLLIPPSNVL
                     IVFSLTAGGVSVASLFMAGYLPGILMGLAVMIVCGIIAKRRGYPLSERATFAQACKAF
                     LDALPSLLLVFIVMGGILGGILGGIFTATEASAIAVVYTFILSVLIYREVKWRHLPKL
                     ILESVVTTSIVLLLIGFSVGMSWAMTNADIPYMISDALMGISDNPLIILLLINIVLLI
                     VGIFMDMTPAVLIFTPIFLPIAQELGMDPVHFGIMMVANLCIGLLTPPVGSALFVGCS
                     ISGVKIQHLIKPLLLFYAALLVALMMITYIPQISLFIPQLLGLM"
BASE COUNT         3795 a         3472 c         3721 g         3487 t
ORIGIN      
        1 gctgaggagt ccccagaaaa gaagacaggc atagagtttt gtgatctact gattgcgcta
       61 acaaattcaa tgagatcacc tctatgcctg cccgttaagt atgtcaggct tttttccgta
      121 atgctctaac ttccacgcat tggtttctga catcaaataa ggctatgact ttctctccct
      181 tttaatctca ttctgatcta aaaccatatc ttaaaaaaat gcatgggtat tttaattatt
      241 ttgatgttga tctaacgcac tcaaggacca ttttattacc gattatccat tctatttaac
      301 tgttgcgata taaatgtttg ttttaggtgc tttaattcag tacaagtcat caaaccgtac
      361 cagaccctga ctgtcagtga caccgacagg tgggggcggc ggtgtggggt caaactgagt
      421 gtgtctgcct ctggcccaca gagccagctc tgccgccatt gcccgtggat gatactccag
      481 ctgcacgttg agaccggaaa ctgggttgtt gttctcatcc acgaactgca gcgccagcca
      541 gtgcatccgc tgcttaccgc aggcgacttc tttgtacttc attttgtgac tcaggcaggt
      601 gactgtcgtg ttgcctgttg cgtccatttt gcggttaaac ccttttaatt caatttgtta
      661 aattgctttc caccttttgc ggggcgcgac ttaaccaaaa tattcactgt tatcataata
      721 tgcaatgggc tggaggttgc aggtcattta gggattttct gtaagccccg ttttgcagaa
      781 cgttctatct ccgattgaga tcctgtctct cggtattacc ggtagtctgg ccataatccc
      841 ctatggatca tattgatata gtaggcataa cacccaaaag ttgactaaag ctaaatcaat
      901 cagagtgaaa aatgttcggt ttgatgtagg gggggactga ggaggggcca acgcaggtgg
      961 tatgcgtttt acttcagacc ggacaaagac cggatgtatc cgtgcagtaa tgaatccgtt
     1021 agccaggtgc tcatgtggaa atagtgtcta ttggggattt gaagtttgac agaaaaggcc
     1081 acagtgacca gaagggggct atttagaaga aatgttcagg ctggatggaa gaggtaaagc
     1141 agtgacatca gcattcaaag tgaccggata tctaccagaa ccccagaaag caaaaaacca
     1201 gcccgtaggc tggttcttct aaatagtggt gcccggactc ggaatcgaac caaggacacg
     1261 gggattttca atcccctgct ctaccgactg agctatccgg gcaacggggc gcattaaacc
     1321 tgattcgcct cgtctcgtca atcaaatttc ttcaatttac tgcagactgc acaatctatc
     1381 atcactttgc cgctattgca cgcattgtca ggcaaaccag gcggtccagg cggcggaaag
     1441 cggcattgcc agaaggagtg ctggaagcat gttgaccacc gggaacattt taatgccaca
     1501 gatgcgtaat ccggttgcca gcagcaataa accgcctacg gcgctgaagt ctgccatcat
     1561 cgacggtgtg atcagcggta atatcagcgc ggcagcccac gccagcgtta actggatgat
     1621 cagtaatggg atactaatta ccgacaccgc aatacccagc gagcaggcga agatcatcgc
     1681 cgtaaagaaa tcaagaaatg acttggcgat taaaatactc ggatcgccgg tcatcccttc
     1741 gttcatcgcc ccgaagatcc cggtgccgct ggcgcaaaac aggacaataa tcgcgacata
     1801 attctgaata aaagattcat gcgctggctt cttacgtgag tgacgaaaca gattttgtgc
     1861 tttggcgact gctgtattga cacctttctc cagcaaacaa atttcgccaa ttagagcgcc
     1921 aagtagcgta gctaaaacca tcgctggaag gttggcgcat ttcacaacca gtaaaatacc
     1981 aatccccagc gatgccagac caaaaataga tgtcatggag acgcggatac gttccggtaa
     2041 gcgttggctg agcagtgcgc cgagaacgcc acccagtaag actgcactag cgttgataaa
     2101 agggccgatg accacaagag ttcctgttag ctgtattgtt tgattttctt attatgaact
     2161 ttttgccttg ttgatggctt gtctggtcgc aaagtcctgg cttgcacgcc ttagcgaaag
     2221 gtgccatgat tgcgcgaatt ttctcctctc tgtacggagt ttgcccgatg cacgccatct
     2281 ccttacattc tctcgcttat cgccgtttcg cgcgaaacgt ttccctttct atgttactgc
     2341 tcatgcggtg aagttaacgc acgctcactg caggacaaca gtaaaatcag agcgtttctg
     2401 cttttactga tgtctggcgg tcggagctgg tgaccagttt gacccatatc tcatggggca
     2461 gggttttcca ccttgtccgg tattcttact tccccgaaac gggtttgcgc ttatgaaatc
     2521 aatgaatatt gccgccagta gtgaagtggt atcccgactt tcttctcatc gccgcgtggt
     2581 ggcgttggga gatactgatt ttacggacgt cgcggcagtc gtcattaccg ctgcggatag
     2641 tcgcagtggc attcttgcgt tgcttaagcg caccggtttt catctaccgg tgtttttgta
     2701 ttccgaacat gctgttgaat tacctgcggg cgttacggcg gtaatcaacg gcaacgagca
     2761 gcagtggctg gagctggaat ccgcagcctg tcagtatgaa gagaatttgc tgccaccgtt
     2821 ttatgacacg ctgacgcagt acgttgagat gggcaacagc acctttgctt gccctggaca
     2881 tcaacatggt gcgttcttta aaaagcatcc tgccggacgc catttttacg atttctttgg
     2941 tgagaacgtc tttcgcgccg atatgtgtaa cgctgacgta aaattgggcg atctgcttat
     3001 tcatgaagga tcggcgaaag atgcgcagaa attcgcagcc aaagtctttc atgccgataa
     3061 aacctatttt gtgctgaacg gcacatcggc agcgaataaa gtggtgacga atgcgctgtt
     3121 aacgcgtggc gatctggtgc tcttcgaccg taacaaccat aagtcgaatc atcacggcgc
     3181 gctgattcag gcggggacga cgccggtcta tctggaagct tcacgtaacc cgtttggttt
     3241 cattggcggt attgatgcgc actgttttaa tgaagagtat ctgcgccagc aaattcgcga
     3301 cgttgcgcca gaaaaagccg acctgccgcg cccgtttcgc ctgacgatta ttcagctggg
     3361 aacctatgac ggcactgtct ataacgcccg tcaggtgatc gataccattg ggcatctgtg
     3421 tgattacatt ctgtttgatt ccgcgtgggt cggttacgaa cagtttatcc cgatgatggc
     3481 ggatagctcg ccgctgctgt tagaacttaa cgaaaacgat ccggggattt ttgtgaccca
     3541 gttggtgcac aaacagcagg ctggattctc acagacgtcg cagatccata aaaaagataa
     3601 ccatatccgc ggacaggcgc gtttttgccc gcataagcgg ttgaataacg cctttatgct
     3661 ccatgcttct accagccctt tctatccgct gtttgctgca ctggatgtta acgccaaaat
     3721 tcatgaaggg gagagtgggc gtcggctgtg ggctgagtgc gttgagttgg gtattgagtc
     3781 gcgcaaggct attcttgcgc gctgtaagct gttccgcccg tttatcccgc ccgttgttga
     3841 tggcaaattg tggcaggatt atccgacatc agtgttagcc agcgaccgcc gttttttcag
     3901 ttttgagccg ggggcgaagt ggcacggctt tgaaggatat gccgcggatc agtattttgt
     3961 tgatccgtgc aagctgttac tcactacacc aggtatcaat gccgaaaccg gcgaatatag
     4021 cgactttggc gttccggcga cgattctggc gcactatctg cgtgagaacg gcattgtgcc
     4081 ggagaagtgc gatctcaact ccattctgtt cttattaact ccggcggaaa gccacgagaa
     4141 gctggcacaa ctggtggcga tgctggcgca atttgaacag catattgagg atgactcgtc
     4201 gctggctgag gtgttgccga gcgtttataa caaatatccg gtgcgctatc gcgactacac
     4261 cctgcgccag ttgtgtcagg agatgcacga tctgtatgtc agtttcgacg tcaaagacct
     4321 acaaaaagcg atgttccgcc agcagagttt cccgtcagtg gtgatgaacc cccaggatgc
     4381 gcatagcgct tatattcgcg gtgaagtgga gttggtgcgg attcgtgatg ccgaagggcg
     4441 aattgcggca gaaggggcgt tgccttatcc acctggcgtg ctttgcgtgg tacccgggga
     4501 agtctggggt ggggcggttc aacgttattt ccttgcgctg gaagaaggcg tgaatttgtt
     4561 gccgggattt tcgccggagt tgcaaggtgt ctatagcgaa accgatgcgg atggcatgaa
     4621 acgattgtac ggttatgtgt tgaagtaaga ataaaaaaac gggtcacctt ctggcgaccc
     4681 gtttttcttt gcgtaattag tggctaaccg tctgtgtgcc tgtcgggaca cgaacgtgtt
     4741 tatatttgaa catcgccatg aacgcgaagg ccagaaccac ggaataacca gcgaaaatca
     4801 accatacggt ctgccagtcg gtaatgccgt tttgggtgta catctcaaca actttaccgc
     4861 tcacgatgcc gccgaggata cagccgaagc cgttagtcat catcaggaac atcccttgtg
     4921 cactggcgcg aattgccggg ctaacttctt tttcgacaaa caccgaacca gagatgttga
     4981 agaagtcgaa tgcgcaaccg taaacgatca tcgacagtac cagcagtaca gtaccgaacg
     5041 gagtcgggtc gccgtaagca aacagcgcaa aacgcaggat ccacgccaca atactgatca
     5101 tcattacgtt cttaataccg tagcggctta agaagaacgg gatggtcaga atgaacaggg
     5161 tttcagagat ctgcgaaatc gacatgatga ttgacgcatg ctgcacgatg aagctgctgg
     5221 caaacatcgg atctttgtcg aagctgtgca ggaaggtatt accgaacatg ttggtaatct
     5281 gcagttccgc gcccagcagc attgagaaga tgaagaagat tgccatacgc ttgtttttaa
     5341 acagcgcgaa tgcatcgagg cccagcaggg ttgtccagct ctgattcgct tgctgtttag
     5401 caaccggaat atgcggcaga gtcagggtaa acagaaccag aatggcggaa agtgctgcgc
     5461 caatatacag ctgcatgtgg cttaattcga agccagacag gctcaccacc cacattgcca
     5521 tgataaagcc gatggtgccc cagatacgga ttggcgggaa gtcagtaacg atatccatcc
     5581 cggcattttg caggcgatag taagagatgg tgttgattaa cccaagcgtt ggcatataag
     5641 caaacgagtt aatcaatatc acaaggaaca tcgcttccgg cgtcgtgacc tgtgccgcca
     5701 tgaacagcgt gatagcgcca atggtgtggc aaatggcata tacccatttc gcacttaacc
     5761 atttgtcggc cacaatcccc agcagcgcag gcataaatac cgcggcgata cccagtgagc
     5821 tataaactgc gccaatagaa gcaccgtcaa acttcagggt aacaaacata taggagccga
     5881 gggtcgtcag ccaacttccc cacagacaga actgcagaaa agagaggatt ttcagctgca
     5941 gcttaagatt catgttaatt tcctcacatc gtgatgcgga tgagtgtttg aaaaagcaca
     6001 tttgaccgtg cgagatgatg ggattctatc aacttcgcgg actggttttt tgttacctgt
     6061 ggcaaataat tgcaaacatt cccaatttgc aaaacgaaat tgtgatgtgg ataacatttt
     6121 tgcccctgag catcgtcagg ggcggttaat ggaattatcg gcggcggtag gatttttgcg
     6181 cggtattcac tttataaaga taacggcgag attccgcaga gggatggcgg gtcgtcagcg
     6241 tctgataaac atcgcccggc gtcatggtgt taataatatt ggcagcctga atcttatcat
     6301 tcgaaaagac tcgcagcacg ctgcctgcgc cgccgttata ggcggtgatg acggcataac
     6361 gccgcgatgt tgggttatca attccgccga gataaacatt gttcagcatc gccagatacg
     6421 cggtgccggt atcaatattg ctggcaggat caaacaagaa actgcggctc ggcgtgccgg
     6481 atttcccctg cgagcggaac acatctttcc cggcagtatt ttgtaccacc tgcattaatc
     6541 ccagcgcatc ggaacggctg accgcatacg ggttaaagga agattcagtc tgcataattg
     6601 ccagaatcag cgactcatca acgccatatt tccgtgacgc ctggcggacc atgccgagat
     6661 atttgtgcgc acgtttatca aggtggttcg gcaccatgtt aatggtgacg ctgtagatga
     6721 tacgtagccc gttgctgcgg cttttcagac ggtttttcag cagataatcc gcgaagttgc
     6781 ttgcgcgacc ttcccagcga atcggctgcc cggtgttgtc caccacctga ccgtaaagga
     6841 aaggttcttt cgaaattgta atatcatcaa cgtcggaata gagatcgacc gaactcggat
     6901 cgtcacccat cagcaacgtt ttgataattg cccggcgcaa atgcgcggca ggttctgtcc
     6961 cggcgatggt ttcgatagta atcgtaccgt catcgaagtt gatgtggctg cgggtctgat
     7021 attgatcggt gtatttcacg tagtccttag gaccggcgat caccacctct ttgaagcccc
     7081 agatgttctc aatattgtgg gcaaattgcc ccatcagaat atcaaaaccg ttggtatctt
     7141 tgacccaggc ttcgttatag gtatcgcctt ttttggtcgt cgaacaggag atgagcaacg
     7201 gcgcaatcaa agccagcgcg agatattttt tcatcattcc gggtgcgtgt tgtgtttgtt
     7261 tttatgcaag ctgccggagg cgtgctccgg cactgttttt attttttatc ttccggcgta
     7321 tagccctcga tatgcacctc tttaccctcg aacaggaagt tgaccatctc ctgctccagc
     7381 agcttgcggt gctcggcatt catcatgttg agtttctttt cattaatcag catggtttgt
     7441 ttgtgctgcc actgcgccca ggcttctttg gagatctcgt tatagatgcg ttttcccagc
     7501 tcgccggggt acagctgaaa atcctgacct tctgcttcac gttgcaggaa agtacaaaaa
     7561 atcgttctgc tcataaatca tcctctttat cgactcacgc gctaaaccgg cgcgccagtg
     7621 cgtaactgct gtaataaacg ctccacggga gccgccaggc caactgacgg cggttgcgct
     7681 aagttatacc agagcgcatt gccttcatcc atgcagccgg tgaatgacga cacgggaagc
     7741 cacataggca caatatctaa gtggaaatgg ctgaaggtat ggcgaaacgc ggtcagttgc
     7801 gtcaggttat cggcagcaat ctgccgttgt gccagccaat gccgcaaact ttcttcgtcg
     7861 gcaaactgcg ggaaacagta taaaccgccc cacaatccgc tcggcggacg ctgcgccagc
     7921 aatacttcat cttcgtgctg taacaggaga aagtagccgg tgcgctccgg cagcgtctgt
     7981 ttcggttttt tgcccggata aagcgaccag ctattgttgg tggcggcaat acatccgttt
     8041 tgtagcggac agagcgaaca tttcggcttc gaacgcgtgc aaatcatcgc gcccaaatcc
     8101 atcatcgcct gattaaaccg ttccacgccg accgcgggcg tcacctgctc gctcaaactc
     8161 catagtttat tctcgacctc ttttttccca ggccagccgc ttacagcata gcagcgcgcc
     8221 agcacgcgtt tgacgttacc gtcgagaatc ggaaagtgct tacccagaga aagcgagaga
     8281 atcgcgcctg cggtggaacg cccgacgccc ggtaacgccg cgacttcttc aaaggtttcc
     8341 gggaatttac caccgtgtaa ggtcgccact tgttgtgccg ctttatgcag gttgcgcgcg
     8401 cgggcgtaat agccaagccc ggtccacaag tggagaactt cgtcgagcgg cgcattggcg
     8461 agatcggtca ccgtcgggaa gtgcgccata aagcgttcaa aataggggat aacggtcgca
     8521 acctgagttt gttgcaacat cacttctgag agccatactt tgtagggcgt cttgtcaatt
     8581 tgccagggca gcgttttccg cccgtattta tcgtaccagt ccagaacctg ggctgaaaat
     8641 tgcgacgctt gcatggtcac cgacttcact gttgttgggg gcaagattgc agcacagcga
     8701 cggcagggtg taaaccggaa ctttccgcag ccacggcctt atcatgcttg catccggcaa
     8761 ctaactttgg ataatgcccg ttttcagaac actttcacaa gcgactaaat ctttatgaaa
     8821 aacgacgtca tttcaccgga atttgatgaa aacggccgcc cactgcgccg tatccgtagt
     8881 tttgtgcgcc gccaggggcg actgaccaaa ggccaggaac atgcgctgga aaactactgg
     8941 ccggtgatgg gcgttgagtt cagtgaagat atgctggatt tccccgcgct ttttggccgt
     9001 gaagcgccgg tgacgcttga gattggtttt ggcatggggg cgtcgctggt ggcaatggct
     9061 aaagatcgcc ccgagcagga cttcctcggc attgaagtgc attcaccggg cgttggtgcg
     9121 tgcctggctt ctgcgcatga agaaggttta agcaacctgc gcgtgatgtg tcacgatgcg
     9181 gttgaagtgc tgcataaaat gattcctgac aattcattgc gcatggtgca gctctttttc
     9241 cctgacccgt ggcacaaagc gcgccataat aaacgccgta tcgttcaggt gccgtttgcc
     9301 gaactggtaa aaagcaaact gcagctgggg ggcgtattcc atatggcgac cgactgggaa
     9361 ccttatgcgg aacatatgct tgaagtgatg tcttctattg acggttataa aaacctgtca
     9421 gagagcaatg attacgtacc gcgtccggca tcacgtccgg tgacgaaatt tgaacaacgt
     9481 ggtcatcgtc ttggtcacgg agtatgggac ttaatgttcg agagggtgaa ataatggcaa
     9541 agaaccgtag ccgtcgtctg cgtaaaaaaa tgcacatcga cgaattccag gaattaggat
     9601 tttcggtggc atggcgattc ccggaaggta catcggaaga acagattgat aaaatcgttg
     9661 atgactttat taacgaggtt atcgaaccga acaaactggc ctttgacggc agcggttatc
     9721 tggcctggga aggtctgatc tgcatgcagg aaatcggcaa atgcaccgaa gaacatcagg
     9781 cgattgtgcg taagtggctg gaagagtgca aactggatga ggtacgcacc agcgaacttt
     9841 tcgacgtttg gtgggactaa gaaagcatac gggcgatgac aaatgcaaaa ctgcctgatg
     9901 cgctacgctt atcaggccta cagagatgca cgatcgagta ggcgggataa ggcgtttacg
     9961 ccgcatccgg catggaaaac gcgtactttg ttatcaatct ggggccagca tatgctggcc
    10021 tgatttgttc ttgagggaag actatgatgc gcaaaatgct gctggcggca gcactttcag
    10081 tgacggcaat gaccgctcac gccgactacc agtgcagcgt cacgccgcgt gacgatgtga
    10141 ttgtcagccc gcaaaccgtg caggtgaagg gcgaaaacgg caatctggtg atcacgccag
    10201 acggcaacgt gatgtataac ggtaagcaat attccctgaa tgccgcccag cgcgagcagg
    10261 cgaaggatta tcaggctgaa ctacgtagca cgctgccgtg gattgatgaa ggcgcgaaaa
    10321 gccgcgtcga gaaagcccgt attgcgctgg ataaaattat cgttcaggag atgggcgaca
    10381 gcagcaaaat gcgcagccgt ctgaccaaac ttgatgcgca gcttaaagag cagatgaatc
    10441 gcattatcga aacgcgcagc gatggcctga cgttccacta taaagccatt gatcaggttc
    10501 gcgccgaagg ccagcaacta gtgaatcagg caatgggcgg aattttacag gacagcatta
    10561 atgaaatggg cgcgaaagcg gtgctgaaaa gcggcggtaa cccattacag aatgtgctgg
    10621 gaagcttggg cggtctgcaa tcctcaatcc aaaccgagtg gaaaaagcag gaaaaagatt
    10681 tccagcagtt tggcaaagat gtttgtagcc gcgttgtgac tctggaagat agccgcaaag
    10741 ccctggtcgg gaatttaaaa taatcctcta ttttaagacg gcataatact tttttatgcc
    10801 gtttaattct tcgttttgtt acctgcctct aactttgtag atctccaaaa tatattcacg
    10861 ttgtaaattg tttaacgtca aatttcccat gcaaagctaa gggataatgc gtagcgtcca
    10921 cgtaactgga ggaatgaaat ggagtttttc aaaaagacgg cacttgccgc actggttatg
    10981 ggttttagtg gtgcagcatt ggcattaccc aatatcacca ttttagcaac cggcgggacc
    11041 attgccggtg gtggtgactc cgcaaccaaa tctaactaca cagcgggtaa agttggcgta
    11101 gaaaatctgg ttaatgcggt gccgcaacta aaagacattg cgaacgttaa aggcgagcag
    11161 gtagtgaata tcggctccca ggacatgaac gataatgtct ggctgacact ggcgaaaaaa
    11221 attaacgccg actgcgataa aaccgacggc ttcgtcatta cccacggtac cgacacgatg
    11281 gaagaaactg cttacttcct cgacctgacg gtgaaatgcg acaaaccggt ggtgatggtc
    11341 ggcgcaatgc gcccgtccac gtccatgagc gcagacggtc cattcaacct gtataacgcg
    11401 gtagtgaccg cagctgataa agcctccgct aatcgtggcg tgctggtggt gatgaacgac
    11461 accgtactgg acggtcgcga tgtcaccaaa accaacacca ccgacgtagc gaccttcaag
    11521 tctgttaact acggtcctct gggatacatt cacaacggta agattgatta ccaacgtacc
    11581 ccggcacgta agcacaccag cgatacgcca ttcgatgtct ctaagctgaa tgagctgccg
    11641 aaagtcggca tcgtttataa ctacgctaac gcatccgatc ttccggctaa agcactggta
    11701 gatgcgggct atgatggcat cgttagcgct ggtgtgggta atggtaacct gtataaatcc
    11761 gtgttcgaca ccctggcaac cgccgcgaaa aacggcactg cagtagtgcg ttcttcccgc
    11821 gtaccgacgg gtgctaccac tcaggatgct gaagtggatg atgcgaaata cggcttcgtc
    11881 gcctctggca cgctgaaccc gcaaaaagcg cgcgtcctgc tgcagctggc tctgacgcaa
    11941 accaaagatc cgcagcagat ccagcagatt ttcaatcagt actaatcacc tcgtcccggc
    12001 atcgtgccgg ggctttttca cttcagactt acgtccattg ccaattttca ttaccctaat
    12061 gataatcacc ggaataaatt attccgcgcg agggttttcg ggtgaaaaag caatggattg
    12121 ttggcacggc gctgcttatg ttgatgactg gtaatgcctg ggcagatggc gaaccgccaa
    12181 ctgaaaatat cttaaaagat caattcaaaa agcagtatca cggcattctc aagcttgatg
    12241 ccatcagctt aaaaaatctt gatgctaagg gtaatcaggc cacttggtca gcggaaggcg
    12301 atgtctcttc cagtgacgat ctctatacct gggtcggtca gttggcagat tacgaactgc
    12361 tcgaacagac ctggacgaaa gataaaccgg taaaattctc ggcgatgtta accagtaaag
    12421 gaacgcccgc gtctggctgg tcggtgaact tttactcttt tcaggcggca gccagcgatc
    12481 gtgggcgggt ggtcgacgat atcaaaacga ataataaata tctgatcgtg aatagcgaag
    12541 atttcaatta tcgctttagt cagcttgaat ctgcgttgaa tacccagaag aactcgattc
    12601 ctgcgctgga aaaggaagtg aaagcgcttg ataagcaaat ggtggcggcg cagaaagcag
    12661 cggatgcata ttggggaaaa gatgcaaacg gtaagcaaat gacccgcgaa gaagccttta
    12721 aaaaaattca ccaacagcgt gatgagttta ataaacagaa cgatagcgag gcgttcgcgg
    12781 ttaaatatga caaagaggtt tatcaaccgg cgattgcggc atgtcataaa cagagtgaag
    12841 agtgttatga agtgccgatt cagcagaaac gagatttcga tatcaacgaa caacggcgac
    12901 agacttttct gcaatcacaa aaactcagcc gtaaattgca ggatgactgg gtaacgctcg
    12961 aaaagggcaa tatccgttaa cgatgaaagt ttcagaaata aacagtaaaa aagtcgcgat
    13021 tttgatgaaa attgacgata tcaaccaagc taatgaacgc tagaaaaaag ataccgaaca
    13081 actgcggcgc aatggggtga ttaagtaact catgttaagt gcctttgccg gataatataa
    13141 aaagttatcc ggcaaattat tttttacatt agccctagta gctgtggaat gaacaaggat
    13201 atttgcggaa tgtaagtgat catcatcaac gctactagca atgcagcata aaatagcagc
    13261 aatggtttga ttaaatgttg aatcttcaca cccgagatag aacaaccaac aaatagcgca
    13321 ctacccaccg gcggtgttaa cagcccaata caaaggttgg ccaccatcat aataccgaaa
    13381 tgtactgggt ccattcccag ctcttgtgca atcggcaaga agatcggtgt aaagattagt
    13441 acagccggtg tcatgtccat aaagatgccg acaattaata agacgatatt aatcagcagc
    13501 aatatgatca gtgggttatc agaaatcccc atcaatgcgt cgctgatcat ataaggaata
    13561 tcagcattag tcattgccca ggacatgccc acggaaaaac cgatcaacag taatacaatc
    13621 gaagtggtaa caaccgactc cagaatcagt ttgggtaaat ggcgccattt tacttcccgg
    13681 taaatcagca ccgagagaat aaatgtgtag acaacggcaa ttgctgacgc ttcagttgcg
    13741 gtaaaaatgc cgcccagaat gccgcccaga atgccgccca tcacaatgaa caccagaagc
    13801 aaacttggta aggcatccag aaacgcttta caggcctgag caaaggttgc tctttctgaa
    13861 agcgggtagc cacggcgttt agcaataatg ccgcagacta tcattaccgc aagacccatt
    13921 aagatccctg gcaaatatcc cgccataaac agcgatgcta cagaaacgcc gccagctgtc
    13981 agggaaaaca caattaacac gtttgagggc ggtatcagta atccagtaat acaagatgag
    14041 acgtttacag cggtagaaaa tgccggatca taaccttctt tggtctgaat gggattcagt
    14101 gtgccaccaa cggcagcagc tgccgcaacg gctgaaccag aaatggaacc aaacatcata
    14161 ttcgccagga cgttcacatg accaagcgaa ccaggaacgc ggccaaccat tacctgggcg
    14221 aggttaatca aacgtatcgc aataccgccg ctgttcatca gtgttccggc aaagatgaaa
    14281 aaggggattg ccaacagggc aaagttatcc agtccattag ccagtcgttg cgaaacagtg
    14341 attgccgcga tatcccaggg aaacatcaaa agcattgaag ctacagtggc aataccgatt
    14401 gagaacgaaa tgggcacacc aataaagacc agcacaaaaa aacagccaaa cagtgtaatc
    14461 gcgatataac tatcc
//
LOCUS       DAJTQY010000095        14250 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.095, whole genome shotgun sequence.
ACCESSION   DAJTQY010000095 DAJTQY010000000
VERSION     DAJTQY010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14250
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.095"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(<1..104)
                     /locus_tag="OO349_003113"
     CDS             complement(<1..104)
                     /locus_tag="OO349_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin"
                     /protein_id="HCR5868099.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGG"
     gene            complement(145..600)
                     /gene="csgB"
                     /locus_tag="OO349_003114"
     CDS             complement(145..600)
                     /gene="csgB"
                     /locus_tag="OO349_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
                     /protein_id="HCR5868100.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
     gene            1353..2003
                     /gene="csgD"
                     /locus_tag="OO349_003115"
     CDS             1353..2003
                     /gene="csgD"
                     /locus_tag="OO349_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HCR5868101.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            2008..2397
                     /gene="csgE"
                     /locus_tag="OO349_003116"
     CDS             2008..2397
                     /gene="csgE"
                     /locus_tag="OO349_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HCR5868102.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTDDLAHDEF"
     gene            2422..2838
                     /gene="csgF"
                     /locus_tag="OO349_003117"
     CDS             2422..2838
                     /gene="csgF"
                     /locus_tag="OO349_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HCR5868103.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            2865..3571
                     /locus_tag="OO349_003118"
                     /pseudo
     CDS             2865..3571
                     /locus_tag="OO349_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001571243.1"
                     /note="involved in the stability of the curlin proteins
                     during assembly; involved in the secretion of the major
                     curlin subunit CsgA across the outer membrane;
                     frameshifted; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
     gene            complement(3704..4195)
                     /locus_tag="OO349_003119"
     CDS             complement(3704..4195)
                     /locus_tag="OO349_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HCR5868104.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(4297..4851)
                     /gene="ycdY"
                     /locus_tag="OO349_003120"
     CDS             complement(4297..4851)
                     /gene="ycdY"
                     /locus_tag="OO349_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HCR5868105.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(4875..5612)
                     /gene="ycdX"
                     /locus_tag="OO349_003121"
     CDS             complement(4875..5612)
                     /gene="ycdX"
                     /locus_tag="OO349_003121"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HCR5868106.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMISTIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPLER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(5667..6605)
                     /gene="ghrA"
                     /locus_tag="OO349_003122"
                     /pseudo
     CDS             complement(5667..6605)
                     /gene="ghrA"
                     /locus_tag="OO349_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
     gene            6840..6927
                     /locus_tag="OO349_003123"
     tRNA            6840..6927
                     /locus_tag="OO349_003123"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6874..6876,aa:Ser,seq:gga)
     gene            complement(7077..7358)
                     /locus_tag="OO349_003124"
                     /pseudo
     CDS             complement(7077..7358)
                     /locus_tag="OO349_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            complement(7640..8644)
                     /locus_tag="OO349_003125"
     CDS             complement(7640..8644)
                     /locus_tag="OO349_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISSfl8 family transposase"
                     /protein_id="HCR5868107.1"
                     /translation="MQNITLIGIDLGKHSFHIHCQDKSGKALLRKKFTRTKLMQFLAT
                     CPPSVVVMEACAGAHFMARCISDFGHETKLISPQFVRPFVKSNKNDFVDAEAICEAAS
                     RPSMRFVQPRTETQQAMRALHRVRESLIRDKVKTTNQIHGFLLEFGISLPIGDAVIKR
                     LSLVLAEHEIPEYLSRLLVRLHTHYFYLVEQIAELESELSQSINADDTAQRIMTIPGV
                     GPITASLLSSQLGDGKQFSCSRDFAASTGLVPRQYSTGGKSTLLGISKRGDKNLRRLL
                     VQCARSFIMRLERKHGKLAEWVREQLNKKHSNVVTCALANKLARIAWAITTQKKEYQA
                     "
     gene            complement(8716..9276)
                     /locus_tag="OO349_003126"
                     /pseudo
     CDS             complement(8716..9276)
                     /locus_tag="OO349_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            complement(9361..9558)
                     /locus_tag="OO349_003127"
     CDS             complement(9361..9558)
                     /locus_tag="OO349_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5868108.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCGSGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            complement(9570..10061)
                     /locus_tag="OO349_003128"
     CDS             complement(9570..10061)
                     /locus_tag="OO349_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5868109.1"
                     /translation="MMKLALTLEADSVNVQALNIGRIVVDVDGIELAELINMVCDNGY
                     SLRVVDESDQTSAECTPPFATLTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHF
                     TGSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPGLPT
                     FDW"
     gene            complement(10058..10432)
                     /locus_tag="OO349_003129"
     CDS             complement(10058..10432)
                     /locus_tag="OO349_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HCR5868110.1"
                     /translation="MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFV
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            complement(10479..10853)
                     /locus_tag="OO349_003130"
     CDS             complement(10479..10853)
                     /locus_tag="OO349_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HCR5868111.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLYYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRYQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPAPATTS"
     gene            complement(11016..11237)
                     /locus_tag="OO349_003131"
     CDS             complement(11016..11237)
                     /locus_tag="OO349_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HCR5868112.1"
                     /translation="MRIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(11300..11776)
                     /locus_tag="OO349_003132"
     CDS             complement(11300..11776)
                     /locus_tag="OO349_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HCR5868113.1"
                     /translation="MQQLSFLPGNMTPGERSLILRALQTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            complement(11792..12265)
                     /locus_tag="OO349_003133"
     CDS             complement(11792..12265)
                     /locus_tag="OO349_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HCR5868114.1"
                     /translation="MKTLSQNTTASACAPETGLQQLVATPVPDEQRISFWPQHFGLIP
                     QWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMNGN
                     RAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            complement(12607..13428)
                     /locus_tag="OO349_003134"
     CDS             complement(12607..13428)
                     /locus_tag="OO349_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HCR5868115.1"
                     /translation="MRLASRFGYAANQIRRDRPLTHEELMHYVPSIFGEDRHTSRSKR
                     YAYTPTITVLESLQREGFQPFFACQTRVRDPGRREYTKHMLRLRRAGQITGQHVPEII
                     LLNSHDGSSSYQMLPGYFRAICTNGLVCGQSLGELRVPHRGNVVEKVIEGAYEVVGVF
                     DRIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLW
                     SAYQTIQENMLKGGISGRSAKGKRIHTRAIHNIDTDIKLNRALWVMAETLLESLR"
     gene            complement(13528..13701)
                     /locus_tag="OO349_003135"
     CDS             complement(13528..13701)
                     /locus_tag="OO349_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HCR5868116.1"
                     /translation="MSRLLPEVPFSREQAVVITTAYRNVFIDDDQGTHFRRVIRNAET
                     DAGRFASDGLLRQ"
     gene            complement(13821..>14250)
                     /locus_tag="OO349_003136"
     CDS             complement(13821..>14250)
                     /locus_tag="OO349_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5868117.1"
                     /translation="SPDTNAATVNQTLKCDSKARADQPGACPTTYELYEGDATYRAAI
                     DKALNPVGLNGMFGEDGYMDGPDGGAYPVNINGTTWVEGGGCKAHACGWDYIVTLYNP
                     KTHKVVGYYYNIDPGYLIWFGETGVHEFAYLVRDYVNKTN"
BASE COUNT         3642 a         3490 c         3411 g         3707 t
ORIGIN      
        1 ccaccaccgt ggttaccgcc gccgccgtac tgaggaacaa cacctgccag agcgctaccg
       61 gagaatacga ttgctgcaat tgctgctact tttaaaagtt tcatgtaaaa cccccatcgg
      121 attgatttaa aagtcgaatg gtaattaacg ttgtgtcacg cgaattgcca tttgcgactg
      181 tctctgcact acaattgccg ttttttgagt accatactgt gtaatatttg ctttattacc
      241 agaacctttc tggataatca tcgcagtatt accataagca ccttgcgaaa tactggcatc
      301 gttggcactg cccgcctgat caatatatgc aaggttataa tctcctgtct ggtcaatctt
      361 tgcccgattg ctactacctt cttgcgcaac aaccgccaaa agttttgagc ctccctgccg
      421 taactgagca ctattattag tcccagcttg accaattatg gctgcctgat taaatgaaga
      481 cttactcaat tcatttaccg cgaagttata ttctgaatta gctaaatcat aacctgctgc
      541 ggctgcaatc ccaggcgcac ccagtattgt taacatcata aacaacaatt tgtttttcat
      601 gttgtcaccc tggacctggt cgtacattta agaaattaaa tcatttcaac ttggttgtta
      661 acgcaacctg tattttgtta acgctgcgtt acgatggaaa gtatgtctgc ggaaatattt
      721 ttaataactc acccgcgcgt tgtatttttc tttttttgtt cataccaaag tattaaaacc
      781 cgcgtaaata cgctgttatc tacgcaaaaa atattttgtt ttctcttaaa tctccgtttt
      841 ccgctatcaa aaatcaccag acagtcattc ttcttgcccg tcgctgattg ctgcgatatg
      901 tcttgcgcac aagccgtgac aaacctcgtc tttttgcagt aaaaaaattg tccacggagg
      961 tgtggagaaa aaacaaaaac gttttacatg acgaaaggac tacaccgaaa tatttttata
     1021 tgtattatta gtaagttagc accatttgta tgatttttta aaattgtgca ataaaaacca
     1081 aatgtacaac ttttctatca tttctaaact taataaaacc ttaatgttaa cattttaata
     1141 taatgagtta cattttgtta catgtttaac acttgattta agatttgtaa tggctagatt
     1201 gaaatcagat gtaatccatt agttttatat tttacccatt tagggctgat ttattactac
     1261 acacagcagt gcaacatctg tcagtacttc tggtgcttct attttagagg cagctgtcag
     1321 gtgtgcgatc aataaaaaag tggggtttca tcatgtttaa tgaagtccat agtattcatg
     1381 gtcatacatt attgttgatc actaaacctt ctttgcaggc gacagctctc ttgcagcacc
     1441 ttaaacaatc gctagcaatt acaggaaaat tacataatat tcaacgttct ctggacgata
     1501 tctcttcagg ctctattatt cttctggata tgatggaagc ggataaaaag cttatccatt
     1561 attggcagga tactttgagc aggaaaaaca acaatatcaa aatattgttg ctaaatacgc
     1621 ctgaagatta cccgtaccgc gacattgaaa actggcctca tatcaacggc gttttttatg
     1681 ccatggagga tcaagaacgt gttgtcaatg ggttgcaagg cgtcctgcgt ggcgaatgct
     1741 actttacgca aaagcttgcc agttacctga ttacacattc tggtaactat cgttataaca
     1801 gcacggaatc agccctcctt actcatcggg aaaaagagat cctgaataag ctgcgtatcg
     1861 gcgcgtctaa taacgagatc gctcgttcgt tgttcatcag cgaaaatacg gttaaaacgc
     1921 atctttataa tcttttcaag aagatagccg taaaaaaccg gacacaagcg gtttcctggg
     1981 caaacgataa cctcaggcga taaagccatg aaacgttatt tacgctggat tgtggcggca
     2041 gaatttctgt tcgccgcagg gaatcttcac gccgttgagg tagaagtccc gggattgtta
     2101 actgaccata ctgtttcatc tattggccat gatttttacc gagcctttag tgataaatgg
     2161 gaaagtgact atacgggtaa cttaacgatt aatgaaaggc ccagtgcacg atggggaagc
     2221 tggatcacaa taacggtcaa tcaggatgtt attttccaga cttttttatt cccgttgaaa
     2281 agagacttcg agaaaactgt cgtctttgca ctgattcaaa ctgaagaagc actaaatcgt
     2341 cgccagataa atcaggcgtt attaagtacg gacgatttgg cgcatgatga attctaaata
     2401 aaaaattgtt cggaggctgc aatgcgtgtc aaacatgcag tagttctact catgcttatt
     2461 tcgccattaa gttgggctgg aaccatgact ttccagttcc gtaatccaaa ctttggtggt
     2521 aacccaaata atggcgcttt tttattaaat agcgctcagg cccaaaactc ttataaagat
     2581 ccgagctata acgatgactt tggtattgaa acaccctcag cgttagataa ctttactcag
     2641 gccatccagt cacaaatttt aggtgggcta ctgtcgaata ttaataccgg taaaccgggc
     2701 cgcatggtga ccaacgatta tattgttgat attgctaacc gcgatggtca attgcagttg
     2761 aacgtgacag atcgtaaaac cggacaaacc tcgaccatcc aggtttcggg tttacaaaat
     2821 aactcaaccg atttttaagc cccagcttca taaggaaaat aatcatgcag cgcttatttc
     2881 ttttggttgc cgtcatgtta ctgagcggat gcttaaccgc cccgcctaaa gaagccgcca
     2941 gaccgacatt aatgcctcgt gctcagagct acaaagattt gacccatctg ccagcgccga
     3001 cgggtaagat ctttgtttcg gtatacaaca ttcaggacga aaccgggcaa tttaaaccct
     3061 acccggcaag taacttctcc actgctgttc cgcaaagcgc cagggcttac aaaacctgct
     3121 taacgagcgc aaggttattc gtgcggcaca agaaaacggc acggttgcca ttaataaccg
     3181 aatcccgctg caatctttaa cggcggcaaa tattatggtt gaaggttcga ttatcggtta
     3241 tgaaagcaac gttaaatcgg gcggtgttgg ggcaagatat tttgggatcg gtgcagacac
     3301 gcaataccag ctcgatcaga ttgccgtgaa cctgcgtgtc gtcaatgtga gtaccggtga
     3361 gatcctctcc tcggtgaaca ccagtaagac gatactttcc tatgaagttc aggccggggt
     3421 tttccgcttt attgactacc agcgcttgct ggaaggggaa gtgggttaca cctcaaacga
     3481 acccgttatg ctgtgtctga tgtcggctat cgaaacaggg gtcattttcc tgattaatga
     3541 tggtatcgac cgtggtctgt gggatttgta aaataaagca gaacggcaga atgacattct
     3601 ggtgaaatac cgccatatgt cggttccacc ggaatcctga cagataaaaa tgccggaatt
     3661 aataatccgg catttactgt ttaagcgaaa gcgtgagtgc ccctcacgct ttatttttga
     3721 tttcctgctc acggtgagcc gtctttgcac tacgcgccgc cagccacagg ccgctgtttt
     3781 tcattgcgta accaaatacc agcccaagcg ccagcgaagg taacaccagt ttccagtcac
     3841 cctgcccggc aaacgtcgcg catgcgccta taaacgtccc cggaacaaat gaaagcagca
     3901 gctgtttggc ctgaatacac atcagaaacg cgacaatacc ggttatgaca taaccgagaa
     3961 tttccagatg tggtgccagt gcactaccgt aaataatgac catcgcccac accacgccac
     4021 taagcagggt tgcagcggag atcgccagcc ctttcagccc accttgcggg caagcaaagt
     4081 aggccgtaca gcccaggaag cccgcccagc ttagtaagcc caaggaaaca gccacccatc
     4141 cccagatacc ggagagaatg cctgttgtga ttgcaataga gagaagtatg ttcatgccgc
     4201 gcatgatagc agaaaacgcg gccatgaatg agatcgagca cacattttat gcaacaacaa
     4261 attatctgtt gcagttatga gtgacgatga tcacatttac tcttcagaat cttcttccag
     4321 ctcgtcccac attgcactaa tggcatcacg ggttagcggt gccatggtgc gccagaaagg
     4381 cgtggttgca tgggcctcca ctttgccgag gaacgcacca caccagggta acagatactc
     4441 actgaacagt gtttccagtg cttcgctctc atcttccgtt gactgatctt ccagccagga
     4501 agccgcaagc agcaatgtgc cgatgtgatc ggctggcgta tccgccagtg gcatccctcg
     4561 ttcggaaaga aaagcgcgca cttccgcttc cgttgcgtcc tcaacccatg cgctacgata
     4621 tggcggcacg gcacattcat cgccgataaa caacgcattg taatcggcag agacttgcgt
     4681 catatcacag cttttctgta aacgcgtcag taactcatcc tgctccagtg gccagttcgc
     4741 agccagtttc ccctcacgaa tcagagtaaa cagcggcacc agtaaaggat cttgcggttg
     4801 gcggtaatag agcgaaccca gcacacgaca gaggatagaa aactcgttca ttaatatatt
     4861 tccattacta gtaattaaag gtctgcaaat tccgcaatcg gtgccatacc gcgagattca
     4921 aggaagttca gtaagcggcg cggagaaaca ttcaaaatgc gctctagcgg aaaatctacc
     4981 gcgtcgagga ttttaagaca ctcttcaaat tcccccatgg taaacgcggt gtgagaatcc
     5041 gagcctaatg ccacccaacc tccagcatca cgtaccgctg cagctaccgc acgacagttg
     5101 tcttcactgc ccttacgtga gtgtaaaaat gaggaattat tgatttccag cgccacctga
     5161 tgtttcgcgg ctgcttcagc aacggcttta acatctattt catatttcgg atttccggga
     5221 tggcttatta tatgcacatt gccgctggcg atagtggaga tcatcgcttg tgtattggtc
     5281 gctttgtcat gtggcgcaaa aaccggctca tgaaaaccgg caataatcag atccagcgag
     5341 tcaaacattt taccgctgca gtcaatttca ccatcaacat ttttaatgtt agcttcaatg
     5401 ccgcgcagga tccctacccc atcaaccact cgcggccaga tacgcatgtt aatgaagtgc
     5461 cagtgatgcg gcgcatcttc catatccggg ccatgatcgg tgatcgcaaa aagtttaatg
     5521 cccttttgtt tggcctgggc aatgtaatca ctcaatgtgc tatatgcatg tgtgctggca
     5581 acggtatgca tatgaaggtc gacgggatac ataactctct cctgtattca attcccgcca
     5641 aggatagcag gaatcctgat gctttattag tagccacgtg cgcggtcgac ttgcccgcag
     5701 accctctccc ctttttcgag ctgggcaata gtgcgagaaa tgtactccac agcttcagcg
     5761 ggacgggtaa tcgcggcgac atgtggtgtt atcgtcacgc gtggatgttg ccagagcgga
     5821 ctttcaggcg gtaagggttc acgattaaaa acatccagca ttgcgccttt aactttcccg
     5881 ctatccagcg ccgcgagcag gtcatcttcc acaacatgaa caccacgcgc caggttgagg
     5941 agatacgcgc catcaggtaa tttttcgagt aattgttgat taataatgcc gacggtttca
     6001 ggggtattcg gtaacaaatt aatcaatacc cgacattggc tcagaaatgc agacagttct
     6061 tcccgtccgg caaagctttg cacgccaggc cacgatttac gggttcgact ccagcaacgc
     6121 agcggaaagc gctaggtttg cagactctga gcaactttac agcccagtac gcctgcgccc
     6181 aaaatgccga tggtaaaatc ttcccgatga tattcaggca gcggttgcca atgcgaacta
     6241 ttttgctgga tgcgataatc gtcaaaacgt cgaaaccaat gcagcacctg actgacagca
     6301 tattcctgca tttgctcgcc cataccggta tcttccaggc gaaaaagtgg aacagaaggg
     6361 ttcagcattt cagggtgtgc ctgtagcttg ctcaaaatag aatcaacacc ggccccgagt
     6421 gcgaacaccg ctttaagatc gcgccctgcc agcatttcaa caggaggatg ccagactaaa
     6481 acataatcag cagagtcatt atctccgctt ttccatgccc tgactcttgc ctgaggaata
     6541 gctttgcgca gtgcctcaat ccaccattgg gtatcgaacg ttgggtgata aaagatgata
     6601 tccatactga ctcccgaaaa gcgttgtgcg aaatttattc gcacttatcg ttatgatcta
     6661 caaaggccac cagcataaca aatccgtggt cggtggcaaa aaaagcagat ttcgcttatt
     6721 aaaaccacac attgattgaa tgtttgaata aacgcgcgat tttttcaaaa agtttgttga
     6781 cctcaggtca tgatttccct aaattagcgc ccgttccagc aagacaggaa cgacaatttg
     6841 gtgaggtgtc cgagtggctg aaggagcacg cctggaaagt gtgtatacgg caacgtatcg
     6901 ggggttcgaa tccccccctc accgccagat tataagtgca tgttattgtt taacatgcgc
     6961 ttttttattt ttccttccag acagtatgcc atcgggtatg ccatctgaaa ccggtttata
     7021 tgccccgcga tcatatctta tcggacaatt tccgatcttt gagcatactg gcaaagctat
     7081 actgccagca tatcaggata gtgttttgcg ataatatcat tcagtctgtc aaccagttct
     7141 ggttttctga acgtgatatg cgcggagccc ttcttaaagt atctgatgct gaacatctca
     7201 tcttcatagc tctctttacc ctgaacagac tgaatgtgat catccagacg aagggtgatg
     7261 tttccccggt tgtcggggat tggtttaccg ctgaacaggt gcaacattct ttccagatcg
     7321 gcgagtcggt cagcctgttg tccggtgata agatgaaatc tatggtcacc cccgtttttg
     7381 caacactgat ttttgattaa tggttggctt gcttaaatct atccggcgtc tctgtgggca
     7441 aggatgcccg cgccccgatg agttccgcac ctgataatcc taaaaatccc ctcggcttca
     7501 gtgagccatt tcatctgtta ggtttttaat caggtcgatt tgccgttcat gtcatcaata
     7561 atcagtcttc gcaaaaccag aaaggtaact tgtatcttat tgtttaaaat aaactaattg
     7621 agttatatca gcatctatat caggcctgat attctttttt ctgagtagtg attgcccagg
     7681 caattcgagc cagtttattt gccagagcgc aggtaaccac attcgaatgc ttcttgttga
     7741 gttgctcccg aacccactca gcaagttttc catgcttgcg ttcaagtcgc attatgaatg
     7801 agcgggcaca ctgaaccagt aaccggcgta aatttttatc acctcgctta ctgataccca
     7861 acagagtcga ttttcctcca gtgctatact gccggggaac aagtcctgtg gaagctgcaa
     7921 aatccctgct gcatgagaac tgtttcccgt caccaagttg cgacgatagt aagctggcag
     7981 taatcggacc aacaccagga attgtcataa tgcgctgagc ggtatcatca gcgttgattg
     8041 actgacttaa ttctgattcc agctctgcta tttgctcaac aaggtaaaag tagtgggtat
     8101 gcaacctcac caaaaggcgg ctcagatatt cggggatttc gtgttcagcc aacacgagag
     8161 acaaccgttt tattacagca tcacctatcg gcaggctgat tccaaattcc agcaagaaac
     8221 cgtggatctg gttggttgtt ttcaccttat ctctgatgag cgattctctg acccggtgta
     8281 acgccctcat ggcctgctgg gtctcagttc ttggctggac aaaacgcatt gatggtcgtg
     8341 aagccgcttc gcatatggct tctgcatcaa caaaatcatt cttattactt ttgacaaacg
     8401 ggcgaacgaa ctgtggagaa atcagcttag tttcatgccc aaaatcagag atacagcgag
     8461 ccataaagtg tgcacccgca caggcttcca ttacaacaac tgagggcgga catgtcgcca
     8521 gaaactgcat cagtttagta cgggtaaatt ttttacgcag aagagcctta ccagacttgt
     8581 cttgacaatg aatgtggaag gagtgctttc cgagatcgat accaataagt gtgatgttct
     8641 gcatgatggt ctctccgtta aagggattta ccttaacagc gtactgctgt taaggtgggg
     8701 gtgaccatat cattatcccc accggtccca tctcaccaga ttgttgacga taattttact
     8761 gccaaattta caggggcaat tggttttgta attccagttc agccctctga agacgttgat
     8821 cacccctcgc tcaaacacct catccttatt ctggtgcagt tgttcaaatg tgctcagaat
     8881 gttcgcttcg ctgatttccg gaaaattatc gtactccagt gaccggtacc atgtatcacg
     8941 ggcctgcgta tccattaatg acagcatccc ggaacgttgc atcaggtcac gccagatttc
     9001 gcgatcaaga ttccgggtaa tggctttcat tgctgtttcg ggtttctcca tcagccagca
     9061 gccgcagcga aaatcctgtt tcatggccca gtccggggca gttttacctc cgatgctttc
     9121 cgtcagcacc gagatttcag ccagttgtcg gataagtgtg tcgatttgca acagtgtact
     9181 ctttcgtaca gccagaatgt ggcgaatgct ggttgagcag ataacgtccg tatgcccgtt
     9241 gaccacttcc ggacgggaga ttgtgatatc tgacataatc gatattcctt tttgcagata
     9301 aatgaaaacg ccagccctgt gcaggctggc gtcagaggaa tgaagtctgg tgagtgagct
     9361 tcaccggttc tggtgcagaa gctgcagatg acgcagggta cggacatcct cccgggcctg
     9421 ttcgatacag ggcaacagtg ctgctgaatc cgttttatcc tcatcgttgt caaagataat
     9481 tcccgatccg cagtcgatat tgtcctgcag ccacgcaatc agaatatcca gcgctgtttc
     9541 cgtggttaat gatttcatgt tgttcctggt taccagtcga aagtgggtaa acccggcagg
     9601 cattcagcgc tggcatccag atgaatgaga ctgacgccat aacgccggat gagtgtgacg
     9661 accagacgac ggaacgtttt tgacagcccc aggcgtttaa gccgcagaac cgggtatgac
     9721 cacgcatcgg tacgtaacag atatccgcta cctgtaaaat gaatccattc tgattcaccg
     9781 aagtcactgg tctggtgtga cagcgagtac aaccaggcgt tgtccgtttc cgtgatatgt
     9841 gcggtactgc agcgtatgcc ggtaagggta gcaaatggtg gcgtgcattc tgctgaggtc
     9901 tggtcagatt catcaacaac acgaagggag tagccgttat cgcagaccat gttaatcagt
     9961 tcagcgagct caataccatc gacgtcaacg acaatgcgcc ctatgttcag tgcctgtacg
    10021 ttaacgctgt cggcttccag tgtcagggcc agtttcatca tttcgcctcc ggatacttac
    10081 ccagggtaat gttatttacc gttctgtaat tgtcgcgggt catcaggccg gtcgcccggc
    10141 gagcccggag gatatcaata ctgtttatta actgagagcg gggacaggtg ctgaatcccg
    10201 gctggtcggt acgcaccagc gcgtattttt ccacgagaaa attaactgca tcacacagtg
    10261 aaatgcctgc ctcaatatgc tgctcaatca cacgttcatc gacaaacggt gtgtcattca
    10321 gtgtgaggcc ataatgctgg tccagcagtc ggatgagcag tgtctgccag atggtgacgg
    10381 gagacgggca gcgagatacc tcccgtacat gtgtgtcgga taatgttttc atcttaagtt
    10441 ctctgtaact ggtagtgcta aaggtgagag cttcggggtt atgaggtggt tgcgggtgct
    10501 ggtgtcggat aaacagccag ataaacgtaa ccacaggagc cgagggtgtc ggcttcgcag
    10561 gtcagccctt ttgcatacag cgtgacggta tgctgatatc ggggattcag ttcaccgctg
    10621 gtgagcatga gttccagttg tttcatcagc agcggaaagg cctggtccag atggtatgca
    10681 tctgcgtcgc tgaagcggcc tctgataccg gcgcggtcgg caaggtaata caggcggtta
    10741 ccctcttgca ccagacgagc gccaaaacag ggcctcactg tgcagggaag cccccaccag
    10801 gggcggtcgt gattgtcatc gggatgagtt atcccggaga gtttgtctga cacgataaaa
    10861 tcctctctga aaagtggtta agaatggttt aacgatgaca ggtcagccgg cgcgctgacc
    10921 ggtttcggtg aagatgtatg cattgatgag aatggcctca tccacagcgg cgtctgaggt
    10981 catctggtcg tactcgtttc ccgggtgacg gaacatcagt tcagggtgac actcatcagc
    11041 cggacatacg ggccaaaact gtccttacgg cgttctgcaa acacggcaag aacgccggga
    11101 atatcctgca cttcacgacc agtatacgct tcagcactgc cgtgccagcg atacttaccg
    11161 gtacagaacg gaaaaagacg ggatgcagga tgctgttggt gaatacgcat ggcttcacca
    11221 cgggtgatga ttctcatgat gtgatacctc tgaagacaga agataaaagt gaaaacaggt
    11281 gtgatgtggt tgtgacgggt taaagcagac catgttcggc aaaggagaaa acctggttgc
    11341 caccgactat cagatggtcc ggcacccgga tatccaccag gcccagtgcc tgtaccagac
    11401 gttcggtgat aagccggtct gccttactgg gtgtgacttc accggacggg tgattgtgcg
    11461 ccagcaccac ggcagcggca ttgtggtaca gggcgcgttt aatcacttcc cggggatgaa
    11521 cttccgtacg gttgatggtg ccggtgaaga gggtttcacc ggcaatcagc tgattctggt
    11581 tgttcagata cagcacccgg aactcttccc gctccagtcc cgccatgttc agaatcagcc
    11641 attcccgtgc cgcacgggtg gaggtgaagg ccacgccggg ttcatgaaga tggcggtcca
    11701 gggtttgcag ggcccgcaga atgagactgc gctcgccggg cgtcatgttt ccgggcagaa
    11761 aggaaagctg ctgcattgtc cctgcctcca ttcagtcgat gatgcgcata atggcgctgc
    11821 attccggatg ctgcagggcg taatcccgca gccggtaata atggaccgtc atggcataac
    11881 actccgtacg acaggcatga tgactgtacg tcatcagaca ggcagcaatg ccggcggctt
    11941 ccgggctcat ttcagcgcgg ttaccgttca tggcattgaa cagtacccat gtttcgtcat
    12001 catcgtcatc cggttcgggt gccataaatg ccccgccgtt gttcagggtg tacagattcc
    12061 agataccacc gcagtagtct tcacacagac ggtccatcca gccgaagaca cggggttcca
    12121 gggtgaccca ctgtggaatg aggccaaaat gctgcggcca gaagctgatg cgctgttcat
    12181 cagggacggg agtggcaacc agttgttgca ggccagtctc cggtgcacag gccgaagcgg
    12241 tggtgttctg agacaatgtt ttcatgtatg aaatccttgt aatgtgaata aatgagaggg
    12301 agtaaaggtc gggagctggc aacatcacgt tcatcactga ccgtgatgac ccggtaatat
    12361 cagggatgaa tgatattcgg ctgaaattcc ggacagccgt acgcgtaacc gttccggtca
    12421 cagacggatg gccagatgcc attgcctgta tactcagtct gcacagactg atttctccgg
    12481 gacggtaaag cagacgggta caccaggcgc acaggtcatc atcagaaatg gaacgacacc
    12541 ccggcagcgc ctccggggcg tcgggtgatt ctggtgcctg aatcacgcct tcagggacat
    12601 gctgcctcag cgcaggctct ccagcagcgt ttctgccatt acccacaatg cgcggttgag
    12661 cttaatatcg gtgtcgatgt tgtgaatggc acgggtgtgg atacgttttc cttttgcact
    12721 gcggccggaa atcccgcctt tcagcatatt ctcctggatg gtctgatatg cgctccacag
    12781 gtctttaccg taatcctccc tgcgtcgtgg tgtcagaatg tcagcggtgg tgactggctg
    12841 atgttcgtca ccataacggt aagtcagtgc cgcctgtgcc agcgcctggc gtgccggtgg
    12901 cggcaggacc agcgactgca tggcatcacg cttctcctct atccggtcaa acacgcccac
    12961 cacctcgtaa gccccttcaa taactttctc caccacattt ccccggtgtg gaacacgcaa
    13021 ttctcccaga gactgaccgc agaccagtcc attggtacaa atggcacgaa aatatccggg
    13081 taacatctgg tagctggatg aaccgtcatg agagttgagc agaataattt cagggacatg
    13141 ctgaccggtt atctgtccgg cccgccgcag acgcagcatg tgttttgtgt attcccggcg
    13201 acccgggtca cgcacacgtg tctggcaggc gaagaatggc tgaaagcctt cccgctgcag
    13261 gctttccagt acggtgatgg tgggagtgta cgcatagcgt ttactgcggg aagtatgccg
    13321 gtcttcaccg aaaatactgg gaacatagtg catcagttct tcgtgtgtca gcggacggtc
    13381 acggcgtatc tggtttgcag cataaccaaa acgactggct agtcgcataa tttgctcctt
    13441 atcggtggtt aagatttact ggtgtaataa atgaaaaagc cacgtctccc ggagaagacg
    13501 cggcctgatg gacgaaatta atgaggttta ttgtctgaga agaccgtcac tggcgaacct
    13561 tccggcgtca gtctctgcgt tgcggataac ccgacggaaa tgagttccct ggtcatcgtc
    13621 gataaacaca ttgcggtaag ctgttgtgat gacaacagcc tgctcgcgtg agaaagggac
    13681 ttcgggcaat aaacgtgaca tacaaccaga cctgtatttg gttccctgaa aatataagtc
    13741 cctgtcatca tggcagagcc gtatttactt ttgatatcaa taaattaata acgcgaagat
    13801 gaaccgcata acttacgcct ttaatttgtt ttgtttacgt agtccctgac caggtacgcg
    13861 aattcgtgaa cgccagtttc accaaaccaa atcaggtaac cagggtcgat gttgtaatag
    13921 taaccaacga ctttatgcgt ttttgggtta tagagtgtta cgatatagtc ccagccgcag
    13981 gcgtgggctt tacaaccgcc accttctacc caggtcgttc cgttaatgtt tacaggatac
    14041 gcaccaccat caggaccatc catatagccg tcttccccaa acattccgtt taacccaaca
    14101 gggtttaatg ctttgtcgat agcagctctg taggtagcgt caccttcgta taactcgtac
    14161 gttgttggac atgcaccagg ctggtcagcc cgggcttttg agtcgcattt aagtgtctga
    14221 tttacagtgg cggcgtttgt gtcaggactt
//
LOCUS       DAJTQY010000096        13965 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.096, whole genome shotgun sequence.
ACCESSION   DAJTQY010000096 DAJTQY010000000
VERSION     DAJTQY010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13965
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.096"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            192..2039
                     /gene="ldtD"
                     /locus_tag="OO349_003137"
     CDS             192..2039
                     /gene="ldtD"
                     /locus_tag="OO349_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR5868118.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPIKGTRWLYSSKPYALSTPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLLYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWEVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADDRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2220..2768
                     /locus_tag="OO349_003138"
     CDS             2220..2768
                     /locus_tag="OO349_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HCR5868119.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2795..3442
                     /gene="gloC"
                     /locus_tag="OO349_003139"
     CDS             2795..3442
                     /gene="gloC"
                     /locus_tag="OO349_003139"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HCR5868120.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3665..4855)
                     /gene="aspC"
                     /locus_tag="OO349_003140"
     CDS             complement(3665..4855)
                     /gene="aspC"
                     /locus_tag="OO349_003140"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HCR5868121.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLSIDGILEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSEIVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCESI
                     VAVL"
     gene            complement(5040..6128)
                     /gene="ompF"
                     /locus_tag="OO349_003141"
     CDS             complement(5040..6128)
                     /gene="ompF"
                     /locus_tag="OO349_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HCR5868122.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            complement(6729..8129)
                     /gene="asnS"
                     /locus_tag="OO349_003142"
     CDS             complement(6729..8129)
                     /gene="asnS"
                     /locus_tag="OO349_003142"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HCR5868123.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(8298..9500)
                     /gene="pncB"
                     /locus_tag="OO349_003143"
     CDS             complement(8298..9500)
                     /gene="pncB"
                     /locus_tag="OO349_003143"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HCR5868124.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            9766..12381
                     /gene="pepN"
                     /locus_tag="OO349_003144"
     CDS             9766..12381
                     /gene="pepN"
                     /locus_tag="OO349_003144"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HCR5868125.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEEGALVISNLPERFTLKIINEISPA
                     ANTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNG
                     NRVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDR
                     GNLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKY
                     VLARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSD
                     LGSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMI
                     HTLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVT
                     VKDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVN
                     SVLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRW
                     DAAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVN
                     EMAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLR
                     NACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKW
                     HQDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAF
                     HAEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLE
                     NLSGDLYEKITKALA"
     gene            complement(12588..13355)
                     /gene="ssuB"
                     /locus_tag="OO349_003145"
     CDS             complement(12588..13355)
                     /gene="ssuB"
                     /locus_tag="OO349_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HCR5868126.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLHLLAGLETPTAGDVLAGTTPLAEIQDDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAAGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKISLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(13352..>13965)
                     /gene="ssuC"
                     /locus_tag="OO349_003146"
     CDS             complement(13352..>13965)
                     /gene="ssuC"
                     /locus_tag="OO349_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HCR5868127.1"
                     /translation="HLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQML
                     RNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARSYG
                     LSGIPLFIHVILPDALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREFLQ
                     TDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATI"
BASE COUNT         3553 a         3562 c         3525 g         3325 t
ORIGIN      
        1 ataacggcgc gcttttcttt aaacttcttt acattaggtt atgtaaaaac gtatcggcgt
       61 ttatatactg aagataagcc tgatgagtaa caggcttgct cgtcatactt tcgtgagtat
      121 tggcgttgta caggcaagtc gtaaaataac agcctggcta ttcagagtat gataaaaaca
      181 gggggcaagg gatgttgctt aatatgatgt gtggtcgtcg gctgtcggca atcagtttgt
      241 gcctggccgt aacattcgct ccactgttca atgcgcaggc cgatgagcct gaagtaatcc
      301 ctggcgacag cccggtggct gtcagtgaac agggcgaggc actgccgcag gcgcaagcca
      361 cggcaataat ggcggggatc cagccattgc ctgaaggtgc ggcagaaaaa gcccgcacgc
      421 aaatcgaatc tcaattaccc gcaggttaca agccggttta tcttaaccag cttcaactgt
      481 tgtatgccgc acgcgatatg caacccatgt gggaaaaccg tgatgctgtt aaagccttcc
      541 agcaacagct ggcagaggtg gcgattgctg gtttccagcc gcagtttaat aaatgggtag
      601 agttactgac cgatcctggt gttaacggga tggcacgtga cgtggtgctc tctgatgcga
      661 tgatgggcta tctccatttc attgcaaata ttccgatcaa aggcactcgc tggctatata
      721 gcagtaaacc ttatgcgctt tcaacgccgc cgctttcggt gattaaccaa tggcagctag
      781 cgctggataa aggtcaattg cctacgtttg ttgcaggact ggcaccgcag catccgcaat
      841 atgcggcgat gcatgaatcg ttactggcct tactcagtga caccaaaccg tggccccaac
      901 tgaccggcaa agcaacgttg cgcccagggc agtggagtaa cgacgtaccg gcgttgcgcg
      961 aaatattgca acgcacaggc atgttggacg gggggccgaa aattactcta cctggcgatg
     1021 acacgccaac tgacgcggta gtcagcccat ccgctgttac tgttgaaaca gcagaaacta
     1081 agccgatgga caagcaaacg acgtctcgta gtaaacctgc gcctgccgtt cgtgccgcct
     1141 acgataatga actggtggaa gccgttaaac gttttcaggc atggcaagga ttgggagcag
     1201 atggtgctat tggcccggca acgcgtgact ggttaaacgt aacgcccgcc cagcgtgctg
     1261 gcgtgttggc actcaacatc cagcgattgc gcttgctgcc aacagagctt tctaccggga
     1321 tcatggttaa cattccagcc tattcgctgc tctactatca gaacggcaat caggtgctgg
     1381 attcgcgagt cattgtcggt cgccccgatc gcaaaacgcc gatgatgagc agtgcactta
     1441 acaacgtagt ggtaaacccg ccgtggaacg taccaccaac tctggcacgc aaagatattc
     1501 tgccgaaagt gcgcaacgat ccgggatatc tcgaaagcca tggctatacg gtgatgcgcg
     1561 gctggaacag cagagaagcg attgacccat gggaggttga ctggtctaca atcacggcct
     1621 cgaatttacc gtttcgcttc cagcaggctc caggcccacg gaactcgctg gggcgctata
     1681 aattcaatat gccgagttca gaggccattt atttgcatga cacgccgaac cacaatctgt
     1741 tcaagcgtga tacacgcgca ttgagctcag gctgtgtacg agtgaataaa gcttccgatc
     1801 tggcgaatat gctgttgcag gatgcaggat ggaatgacaa acgtatttct gatgcgctga
     1861 agcagggtga tacacgttac gtcaatattc ggcagtcgat tccggtgaat ctctactacc
     1921 tgacggcctt tgttggtgca gatgatcgta cccagtatcg tacagatatt tacaattatg
     1981 atctgcctgc gcgatccagc tcgcaaatcg tatcgaaagc ggaacaatta atcaggtaaa
     2041 tgaagtagtt cggggaaatc aattgtcgta ataattagca tgattggggg cgattctctg
     2101 cagcccccgt cactgctggg gttgagtcat cttgacgtct gctttacggg cggttaaggt
     2161 gcctcttgtg cgccagaagt gcatataaac gataacattg agctgtagac ttgattatca
     2221 tggacaaatt cgacgctaat cgccgcaaat tgctggcgct tggtggcgtt gcactcggtg
     2281 ccgccatcct gccgacccct gcgtttgcaa cactctctac cccacgcccg cgcattttga
     2341 cactcaataa tcttcatacc ggagagtcaa tcaaagcgga gtttttcgat ggcagaggct
     2401 atattcagga agaattggca aaacttaacc attttttccg cgattaccgc gcgaacaaaa
     2461 taaagtccat cgacccagga ttattcgacc agttgtatcg cctgcaaggg ttgttaggca
     2521 cgcgcaaacc ggtgcaactc atttccggtt atcgttccat tgataccaac aatgaactac
     2581 gcgcccgcag ccgtggagta gcgaagaaaa gctatcacac taaaggccag gcgatggatt
     2641 tccatattga aggtatcgcg ttaagcaata ttcgcaaagc cgcgttatct atgcgcgcag
     2701 gtggtgtagg atattaccca cgtagtaact ttgtgcatat tgataccggg ccagcacggc
     2761 actggtagta atcgcttaac gaaacagggg cagtatgaac tatcgtatta ttccggtcac
     2821 cgcattctcc cagaactgtt cattgatctg gtgtgaacaa acccgcctgg ccgcactggt
     2881 cgatcctggc ggcgatgcgg aaaaaatcaa acaggaagtt gatgacagcg gcctgacact
     2941 gatgcagatc ctgctgacgc atggtcatct ggaccacgtt ggcgcagcgg cggaactggc
     3001 gcaacattac ggcgtgccgg tttttggccc ggaaaaagaa gatgagttct ggctgcaagg
     3061 cttgcctgcg caaagtcgta tgtttggtct ggaagagtgc cagccgctga cgccagatcg
     3121 ttggctgaac gaaggcgata ccatcagcat agggaatgtg actttacagg tgttacattg
     3181 ccctgggcat acgccgggtc atgtcgtgtt ttttgatgat cgggcaaagc tgctgatttc
     3241 tggcgatgtt attttcaaag gcggagtagg gcgcagtgac ttcccgcgtg gcgatcataa
     3301 tcaactgatt tcttcaatca aagataaatt gctgccactg ggggatgacg tgacatttat
     3361 tccgggtcac ggaccattat ctacacttgg ttatgagcgc ctgcacaatc ccttcctgca
     3421 agacgaaatg cccgtctggt aaaggcgcat aaaaaagtcc acttttaatg cgggcctgga
     3481 tttctggcaa agtgcgcttt gtttatgccg gatgcggcac gagcgcctta tccggcctac
     3541 aaaatcgtgc aaattcaaaa tattgcaggg gacgcgtagg cctgataagc gtagcgcatc
     3601 aggcaatgtt gcgtttgtca tcagtctcag cccgcttttc agcgggcttc attgttttta
     3661 acgcttacag cactgccaca atcgattcgc acagcggagc catgttatct ggtgtcatcc
     3721 cggccacatt tacgcgacca gaagcaacag catatacgcc aaactcttcg cgcagacgca
     3781 gcacttgttc ttttgtcaga ccactgaagg agaacatgcc gttctgtttg atgataaagc
     3841 tgaagtcgcg gtttgcacct ttttcctgca gcgtattgac gaacaactga cgcatacgct
     3901 gaatacgctg gcgcatatca gtcagctctt gttcccaaat cgcacgtaat gcatcgttgc
     3961 tcaggatggt ggcaacaaca gaagcgccgt gtgctggagg gttagagtag ttagcgcgaa
     4021 tcgccgcttt catttggctg aatgcgcgat caacgatttc actgtcggca gcaaccagag
     4081 tacaagcgcc aacacgctcg ttgtacaggc caaagttttt agagtaggaa ctggcaacaa
     4141 tcagctcttt atgcatagcc gcgaaagcgc gcagtccttc agcatcttct tccagaccac
     4201 gggcaaaacc ctggtaagcg aagtcaaaca gcggtaacca gcctttctca acggagagtt
     4261 gtgccagtgt ctgccattgt tccagcgtag ggtcgatacc ggttgggtta tggcagcagc
     4321 catggaacag cactacgtcg ccagcctgag cttcgttcag actgttaatc agtgcatcga
     4381 agtcaagggt gtgattttcc gcatcataat aagcgtattc acgaacttcc agacctgcag
     4441 agttaaagac gctcttatgg ttcggccagc ttgggttgct cacccacaca cgcttaacgc
     4501 tggtattttt tgctaggaag tcggcagcca cgcgtagtgc gccagtgcca cccggagtct
     4561 gtgccgtgcg agcacgtttg tcattgatca gggcgctacc tttaccaaac agcagttcct
     4621 gagtgcagcg accaaattca aggatgccgt caatgctgag gtaatttttg gtggtttcat
     4681 tttcgagcag atactgttca gcctttttca cgctggtcag taccggggtt ttgcccgtct
     4741 catctttata gacaccaatc ccgaggttaa ttttgccggg acgttcatcg gcacgaaaca
     4801 gatcggccag gcccagaatc gggtcggcag gagcggcggt aatgttctca aacatgacga
     4861 ggttccatta tggttacaga agggaagtcc gctatcaggg taacgggaga tttacaaaat
     4921 tccaactatt actgatgaaa acgcaggctg tttttgcaag acgtgagatt gctctggaag
     4981 gcataaaaaa aacaggacca acgtcctgtt ttttcggcat ttaacaaaga ggtgtgctat
     5041 tagaactggt aaacgatacc cacagcaacg gtgtcgtctg aacctacgcc cagtttgttg
     5101 tcagaatcga tctggttgat gatgtagtca acataggtgg acatgttttt gttgaagtag
     5161 taggttgcgc ccacttcaaa gtagttcacc agatcaacat caccgatacc ttctacgtct
     5221 ttcgctttag atttggtgta agcgatggac ggacgcagac cgaaatcgaa ctggtattgc
     5281 gcaactaaca gaacgtcttg cgttttgttg gcgaagccgc tggtgtttgt aaatttatta
     5341 gtgatcggcg tagcgttacg ggtttcaccg tagttcgctg ccaggtagat gttgttcgcg
     5401 tcgtacttca gaccagtagc ccactgttca gcttttttac cgttgccaag aggttgagct
     5461 tcttgcaggt tggtacggtc agctgcacca taagcaccaa cgataccaaa gccttcgtat
     5521 tcgtagctga tagaaccgcc aacaccgtcg ccgttagaac ggcgtgcagt gtcacgctcg
     5581 tttttaccca ggtactgaac agcgaagttc aggccatcaa ccagaccaaa gaagttggag
     5641 ttacgatagg tagcaacgcc gccaacacga ccaacgaaga agtcatcgct gtatgcagta
     5701 tcaccaccaa attctggcag catatcggtg taacccagtg catcataaac cacaccgtag
     5761 ttacggccgt aatcgaaaga accaacgtca gcgtatttaa gacccgcgaa tgccagacgc
     5821 gttttgttac cagtttgagc gtcagcgcct tcagagttgt taccctggaa gttatattcc
     5881 cactgaccat aaccggtcag atcggaattg atttgagttt cccctttaaa accaagacgg
     5941 gcataggtca tgtcgccatt gccaccgtaa ctgttttcac cgttaccctt ggaaaaataa
     6001 tgcagaccaa cagctttacc gtacagatct actttgttgc catctttgtt atagatttct
     6061 gcagcgtttg cagtacctgc tactaacaga gcagggacga tcactgccag aatattgcgc
     6121 ttcatcatta tttattaccc tcatggtttt tttatgacac ctgccactgc cgtcaataag
     6181 ttctgtcaat aaaaatttac ggaactattg atgagagttt ggtgtcttta tgtgtctgca
     6241 ggcatctttc cattcaaact aacgtttcgc taccgtgaaa gtgctacaaa gataaagatt
     6301 tagtttcaaa aagaaaaaat atgtaaccaa aagtaaaaat taaggagctt tgtgaacacc
     6361 gtcatatttc catagaaacg tgatgatatt tacagcaatt tttatctatt tatatgattt
     6421 acttatattt aaattaacta aacggaaatt ttgtttctga tggaaacttt atcgacctgg
     6481 cacaaaatcc tctttcagac atccagaatg ccaaaaaata gtatgaaaat tgtgctatca
     6541 gctaagaaaa aactaatccg caataagaat gcgggttatt ttttgtgcgt aaatgttgca
     6601 atgtaatatt ccgtaacagg atgatcgtta tggcgggggt gcaaatagat tgcttgactt
     6661 aacaaacaga taacttgaca gaaaggataa aaaagagcca gcaggcgctg gctctttgag
     6721 gaagaaaatc agaagctggc gttgcgcgga gtacgtggga acggaatcac atcacgcacg
     6781 ttttgcacgc cagttacgta agcaatcagg cgttcaaaac caagaccgaa acctgaatgc
     6841 ggaacagtac cgtagcgacg cagatcgcga taccaccagt aatcttcttt attcaggccc
     6901 atttccagca tacgctcgtc cagaacgtcc agacgttctt cacgctggga gccaccaatg
     6961 atctcaccga tgcccggagc cagaacgtcc atagccgcaa cggttttacc gtcttcgttg
     7021 aggcgcatat agaacgcttt aatatctttc ggatagtttt taaccactac cggtgcttta
     7081 aagtgttctt ccgccagata acgctcatgc tcagaagaga gatcgacgcc ccagtaaacc
     7141 gggttttcaa acttcctgcc gcagttttcg agaatggtca ctgcgtcggt gtaatccacc
     7201 tgcgcgaaat cggcttcaat gaagcgttcc agacgtgaaa cggcatcttt atctacgcgt
     7261 tcagcgaaga atttcatgtc gtctgcgcgt tcttcgagaa ccgctttgaa gacatatttc
     7321 agcatggctt cagccagacc cgcgatatcg ttcaggttag caaacgccac ttccggctcc
     7381 agcatccaga attccgccag gtgacggctg gtgttggagt tttcagcacg gaaagtcggg
     7441 ccgaaggtat aaattttgga taatgcgcaa gcgtaggttt cgccgttcaa ctggccagat
     7501 acggtcagga aagactcttt accaaagaag tctttgtcga aatccacttt gccctgatcg
     7561 ttacgcggca ggttttccag atccagcgta gaaacgcgga acatttcgcc tgcaccttcg
     7621 gtatcagatg cggtaatcag tggcgttgaa acccagaaga atccctgctc gttaaagaag
     7681 cgatgcagcg cctgcgccag cgtatggcga acgcgcgcga cggcaccaat caggtttgtg
     7741 cgcggacgca ggtgagcgac ttcacgcaga tactcaatgc tgtggcgttt tgccgccatc
     7801 gggtaagtgt ctggatcttc aacccaacca gcaacttcaa ccttgctggc ctgaatttca
     7861 aattgttgcc cctggcccgg cgatgccacg actttacccg tcacaatgac cgagcagccg
     7921 gtggtcagac gcaggacgtc ttcattgtaa ttgggcagag aattattgat gacagcctgt
     7981 acaggatcaa agcaggaacc gtcataaacg gcgaggaagg agatgccagc ttttgaatct
     8041 cggcgggtac gtacccatcc gcgtacggtg acttcgctgt caacggctac gcggccctgg
     8101 agtacgtcgg ctacaggcac aacgctcata atattctctc tgttaatagt cggaaaaaat
     8161 aaacacttgt ccacccgtaa atggggggga tacctatgtt acctggcatc tgcaatcaga
     8221 caagcagaat tcgcaaatgc agcgaaagat ttcggaatta aaggtaaaaa agggagccga
     8281 ttagctccct gatgatatta actggctttt ttaatatgcg gaaggtcgaa cgctttgcgc
     8341 agcgcccgaa caaacgcttt atcatggcag atagttttgc cagggctgtc agaaagtttc
     8401 gccaccggtt taccgttaca ctctaccaac ttaatcacaa tattcagggg ttttacctgg
     8461 gggatatcgc aggtcaggcg agtcccaata ccaaaactta attgcacgcg ggaagagaag
     8521 tggcggtata gctcaaccgc tttgcgtaaa tccagattgt cagagaaaac cagcgtttta
     8581 ctctgtggat caattcccag cttttcataa tgtgcaatgg ctttttcacc ccattcaacc
     8641 gggtcgccag agtcatgacg caggccctga taccgactag cgaactcgac accgaaatca
     8701 cgcaggaaag catccatagt gatgcagtcg gttaatgcaa tgccaagttg gtcgggatac
     8761 tcttccagcc aggcagcaag tgcagctcgc tggctgttgg ctagatccgg gctgatttgc
     8821 tgatgtgcct ggaaccattc gtgtgcctgt gttcccatcg gcgtgaggga aagccgacgc
     8881 gccagatcgt agttgctggt gcccacgaac caggattcct gttgcagacg cttaacgatg
     8941 gtttcttgta cttcgcgaga aaaacggcga cgggtgccaa aatccatcag atggaagcgc
     9001 gacatatcaa gaccggcggt taacgccgag aagtcgacta atttgctttc cagcgtgtcg
     9061 agggcttgcg caacgtcggc ctgcggtgag cgatagcgat gtaccatttc actgataacc
     9121 gccagcaaag gaacttccca gaggatgact tcacgccacg gaccgcttaa gcgaatatcc
     9181 agcttgccat tatcgttgga cacggtgact tgttccgggt taaagcggaa ctcgcgtaac
     9241 cagttaagat agtcggcctt aaagaaaggc agggcagaga gccactgata ttcatcatcc
     9301 tgcaggcgca ggtgctgcat cgcctgaatc tgttcacgaa tagcatcggc ataaataccc
     9361 agcagatcgt cacctcggca acgaaactcc gccgcgacat gcacatcgta atagtgatga
     9421 aacacggctt gctgcatatg caacttataa gcatctgtat ccagcaacga gtgcagaaca
     9481 ggagaagcga attgtgtcat aggtgcgcag tagcgtcctc gtacaggagc gtttagtaca
     9541 ataaacatct tcaggaactg ctggagtata ccttgtttcg caatttattg aaccccgatc
     9601 acaccatatg ccacctttct ggtcgatggc attacgcggt gtatgttata aaaatgtagc
     9661 aataaaggcg tttgtacctg aaaagatgaa gattctgcat agcgcgattt acgcaacagg
     9721 aatagactga acaccagact ctataaaaga tgctaaaggt tatttatgac tcaacagcca
     9781 caagccaaat accgtcacga ttatcgtgcg ccggattacc agattactga tattgacttg
     9841 acctttgacc tcgacgcgca aaagacggtc gttaccgcgg tcagccaggc tgtccgtcat
     9901 ggtgcatcag atgctccgct tcgtctcaac ggcgaagacc tcaaactggt ttctgttcat
     9961 attaatgatg agccgtggac cgcctggaaa gaagaagaag agggcgcact ggtcatcagt
    10021 aatttgccgg agcgttttac gcttaagatc attaatgaaa taagcccggc ggcgaatacg
    10081 gcgctggaag ggctttatca gtcaggcgat gcgctttgca cccagtgtga agccgaaggt
    10141 ttccgccata ttacgtatta tctcgaccgc ccggacgtgc tggcgcgttt taccaccaaa
    10201 attattgccg ataaaatcaa atatcccttc ctgctttcca atggtaaccg cgttgcgcaa
    10261 ggcgaactcg aaaacggacg ccattgggta cagtggcagg acccgttccc gaaaccgtgc
    10321 tacctgtttg cgctggtggc aggcgacttt gatgtactgc gcgatacctt taccacgcgt
    10381 tctggtcgcg aagtagcact ggagctgtac gtcgatcgcg gcaaccttga tcgcgcgccg
    10441 tgggcgatga cctcgctgaa aaactccatg aaatgggatg aagaacgctt tggcctggag
    10501 tatgacctcg acatctatat gatcgtcgcg gtggatttct tcaatatggg cgcaatggag
    10561 aataagggtc tgaatatctt taactccaaa tatgtgctgg cccgcaccga caccgccacc
    10621 gacaaagatt acctcgatat tgaacgcgtt atcggccatg aatatttcca taactggacc
    10681 ggtaaccgag tgacctgccg cgactggttc cagctcagcc tgaaagaagg tttaaccgtc
    10741 ttccgcgatc aggaattcag ctctgacctt ggttcccgcg cagttaaccg catcaataat
    10801 gtgcgcacca tgcgcggatt gcagtttgcg gaagacgcca gcccgatggc gcacccaatc
    10861 cgcccggaca tggtcattga gatgaacaac ttctacaccc tgaccgttta cgagaagggc
    10921 gcggaagtga tccgtatgat ccacaccctg ctgggcgaag aaaacttcca gaaagggatg
    10981 caactctatt tcgagcgtca tgatggtagc gcagcgacct gtgacgactt tgtgcaggcg
    11041 atggaagatg cgtcgaatgt tgatctctcc catttccgcc gttggtacag ccagtccggt
    11101 acgccggttg tgaccgtcaa agacgactac aatccggaaa ctgagcagta caccctgacc
    11161 atcagccagc gcacgccagc cacgccggat caggcagaaa aacagccgct gcatattccg
    11221 tttgccatcg aattgtatga caacgaaggt aaagtgatcc cgttgcagaa aggcggtcat
    11281 ccggtgaatt ccgtgctgaa cgtcacccag gcggaacaga cctttgtttt tgataatgtc
    11341 tacttccagc cggtgcctgc gctgctgtgc gaattctctg cgccagtgaa actggaatat
    11401 aagtggagcg atcagcaact gaccttcctg atgcgtcatg cgcgtaatga tttctcccgt
    11461 tgggatgcgg cgcaaagttt gctggcaacc tacatcaagc tgaacgtcgc ccgtcatcag
    11521 caagggcagc cgctgtctct gccggtacat gtggctgacg ctttccgcgc ggtgctgctc
    11581 gatgagaaga ttgatccggc gctggcggca gaaatcctga cgctgccttc tgtcaatgaa
    11641 atggctgaac tgttcgatat catagacccg attgctattg ccgaagtacg cgaagcactc
    11701 actcgtactc tggcgactga actggcggat gagctgctgg ctatttacaa cgcgaattac
    11761 cagagcgagt accgtgttga gcatgaagat attgcaaaac gcactctgcg taatgcctgc
    11821 ctgcgcttcc ttgcttttgg tgaaacgcat ctggctgacg tgctggtgag caagcaatac
    11881 catgaagcaa acaatatgac cgatgcgctg gcagcgcttt ctgcggcggt tgctgcacag
    11941 ctgccttgtc gtgacgcgct gatgcaggag tacgacgata agtggcatca ggacggtctg
    12001 gtgatggata aatggtttat cctgcaagcc accagcccgg cggcgaatgt gctggagacg
    12061 gtgcgtggtc tgttgcagca tcgctcattt accatgagca acccgaaccg tattcgttcg
    12121 ttgattggtg cgtttgcggg cagcaacccg gcagcgttcc atgccgaaga tggcagcggt
    12181 taccagttcc tggtggaaat gcttaccgac ctcaacagcc gtaacccgca ggtagcttca
    12241 cgtctgattg aaccgctgat tcgcctgaaa cgttatgatg ccaaacgtca ggagaaaatg
    12301 cgcgcggcgc tggaacagtt gaaagggctg gaaaatctct ctggcgatct ttacgagaag
    12361 ataactaaag cactggcttg atacttttga gccaaaatgc ctgatgcgac gcttgacgcg
    12421 tcttatcagg cctacaactc cgttgaaatt gctggttttg taggctggat aaggcgttca
    12481 cgccgcatcc ggcagccgtg cccaactgcc tgatgcgatg cttgacgtgt cttatcaggc
    12541 ctacaagtcc gttgaaaatg ctggttttgt gcgccacatc cggcatatta accatgacgc
    12601 cgaatcagtt gttcagaatg cccacgccgc attacccgtt gcaatacttc cgcttccagc
    12661 tccgctaatc gcaccgaccc taatcgacgc ggacggggga tatccactgt caaatccaga
    12721 ctaatttttc cctcttcaat taacaacacc cggtcagcca ttgctaccgc ttcgctcaca
    12781 tcatgcgtca ccaacagtac ggtaaagccg tgctgctgcc aaagtgacac aatcaaatcc
    12841 tgcatctcga gtcgcgttaa ggcgtccagt gccccgagcg gttcatcaag caacaataaa
    12901 cccggtcgat gaatcaacgc tcttgccagc gccacacgct gtttctgccc gccagaaagt
    12961 gcggcaggcc attccccggc gcgattctcc agtcctgccg cagccaacgc ccgacgcgcg
    13021 gcatcgcgcc actgaccttt aaggcctaac ccaacgttat caatcaccga tttccacggc
    13081 agcagacgcg catcctgaaa catcattcgc gtatcatcct gaatttcagc cagcggtgtg
    13141 gtgcccgcta acacatcgcc tgcggttggc gtttccagac cggccagcag gtgcagcagg
    13201 gtacttttac caccaccgct gcggcccacc accgccacaa actgacctgc cggaatatgt
    13261 aaatccagtt ggttcaggac gatattttcc gcgtaatgtt tgcttactgc attgagcaac
    13321 aatggtgtgc cctggttcag acgagcagta ttcatatcgt ggcctccttc aaatgataag
    13381 ccgggttcca gcgcaaccag aggcgctcta acagctgcgc gctgacgtca gcaagtttgc
    13441 cgagcagggc gtaaagaata atagcgacta ccaccacgtc tgtttgcaaa aactctcgcg
    13501 cattcatcgc cagataacca atgccggaat tggcagaaat ggtttcggca acaatcagcg
    13561 tcagccacat caggcccaac gcaaaacgca cgccgaccat aattgagggc agggcatcgg
    13621 gcaggatcac atggataaac agcggtatgc cggataatcc atagctacgc gccatctcca
    13681 ccagcccgcg atcgatatta cggatgccat gccaggtgtt gatataaatg gggaacagcg
    13741 taccgagcgc caccagaaag attttcgcgg attcatcaat gccaaaccac aaaatcacca
    13801 gcgggatcag cgccagatgc ggcacgttgc gcaacatctg aattgaggta tccagcagcc
    13861 tttctcccca gcgcgacagt ccgctaatca gtcccagaat caggcccagc gatccgccaa
    13921 ttgaaaagcc aatcagcgcc cgccaggaac tgatcgccag atgct
//
LOCUS       DAJTQY010000097        13955 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.097, whole genome shotgun sequence.
ACCESSION   DAJTQY010000097 DAJTQY010000000
VERSION     DAJTQY010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13955
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.097"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            295..1548
                     /gene="kch"
                     /locus_tag="OO349_003147"
     CDS             295..1548
                     /gene="kch"
                     /locus_tag="OO349_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HCR5868128.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLLKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
     gene            complement(1603..1776)
                     /locus_tag="OO349_003148"
     CDS             complement(1603..1776)
                     /locus_tag="OO349_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HCR5868129.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            1919..3379
                     /gene="cls"
                     /locus_tag="OO349_003149"
     CDS             1919..3379
                     /gene="cls"
                     /locus_tag="OO349_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCR5868130.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAH
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            3414..3743
                     /locus_tag="OO349_003150"
     CDS             3414..3743
                     /locus_tag="OO349_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HCR5868131.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(3796..4800)
                     /gene="oppF"
                     /locus_tag="OO349_003151"
     CDS             complement(3796..4800)
                     /gene="oppF"
                     /locus_tag="OO349_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HCR5868132.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(4797..5810)
                     /gene="oppD"
                     /locus_tag="OO349_003152"
     CDS             complement(4797..5810)
                     /gene="oppD"
                     /locus_tag="OO349_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HCR5868133.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            complement(5822..6730)
                     /gene="oppC"
                     /locus_tag="OO349_003153"
     CDS             complement(5822..6730)
                     /gene="oppC"
                     /locus_tag="OO349_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HCR5868134.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTPGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            complement(6745..7665)
                     /gene="oppB"
                     /locus_tag="OO349_003154"
     CDS             complement(6745..7665)
                     /gene="oppB"
                     /locus_tag="OO349_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HCR5868135.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            complement(7751..9382)
                     /gene="oppA"
                     /locus_tag="OO349_003155"
     CDS             complement(7751..9382)
                     /gene="oppA"
                     /locus_tag="OO349_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HCR5868136.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLRYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            complement(10120..10767)
                     /gene="ychE"
                     /locus_tag="OO349_003156"
     CDS             complement(10120..10767)
                     /gene="ychE"
                     /locus_tag="OO349_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HCR5868137.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            11244..13919
                     /gene="adhE"
                     /locus_tag="OO349_003157"
                     /gene_synonym="adhC"
     CDS             11244..13919
                     /gene="adhE"
                     /locus_tag="OO349_003157"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HCR5868138.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
BASE COUNT         3660 a         3454 c         3244 g         3597 t
ORIGIN      
        1 gatccaatat ttaagggcac ggtttttgca accgtgttct tgtttggcct ttccttatag
       61 ccggtcagtg cattcactac cacaagcaga taataacttt atctgtattt ctttatatga
      121 tgtgagcatg ttcaggaaaa aagccctttc ctgacgcaga gttgctcact caaaaccttt
      181 gattactgcg gataaagtca tctactattg tttaacacat tcacgcgctg catgtatgtt
      241 cagaaaatgt gcacgctaag ataattaaac aaaattgtgg ttacagaggt atcagtgagt
      301 cactgggcta cattcaaaca aacagcaaca aatttatggg tgaccctacg gcacgacatt
      361 ctcgcgctgg ccgtcttttt aaatggattg cttattttta aaacaatcta tggtatgtcg
      421 gtcaatttgc ttgatatttt ccatatcaaa gcattttcag agttggatct ctccttgctg
      481 gcaaacgccc cactatttat gctcggcgtc tttcttgtcc tgaactccat tggcttactg
      541 ttccgggcaa agctcgcatg ggcaatcagt atcattttgt tgttgatagc gctaatttac
      601 accctgcatt tttatccctg gctgaaattt agtattggat tttgcatttt tacgctggtg
      661 tttttgctga tactgcgcaa agacttctcc cacagtagcg ccgcagccgg gacaattttt
      721 gcatttatta gtttcacgac attactgttt tactcaacct acggtgcgct ttatttaagc
      781 gaaggtttta atccgcgaat agaaagtttg atgaccgcgt tctatttttc gatagaaacc
      841 atgtcaaccg tcggctatgg agatattgtc cctgtttctg aatcagcacg attgttcact
      901 atttcggtca ttatttccgg cattaccgtt tttgccacat ctatgacctc aatttttggt
      961 ccgcttatcc gcgggggatt caacaaactt ttaaaaggaa acaatcatac aatgcatcgt
     1021 aaagatcatt ttattgtttg cggacattcg attctcgcca tcaatacgat cctgcaactg
     1081 aatcaacgcg gacaaaacgt aacggttatc agcaacttgc ctgaagatga tatcaagcaa
     1141 cttgagcaac gcttaggcga taacgctgat gttatccccg gtgacagtaa tgacagttca
     1201 gtattaaaga aagcgggaat cgatcgttgc cgggccattc tggcgctgag tgataacgat
     1261 gcagataacg cgtttgttgt actctcggca aaagatatga gcagtgatgt caaaacagtt
     1321 ctcgccgtca gtgatagcaa aaacctgaat aagattaaga tggtacatcc ggatatcatt
     1381 ctctcaccgc aactgtttgg cagcgaaatt ctggcgcgag ttttaaatgg tgaagagatt
     1441 aataatgata tgctcgtttc aatgttgttg aactcaggtc atggtatttt cagcgataac
     1501 gatgaacaag aaacgaaagc tgacagtaaa gaatcggcgc aaaaatagag tttaaagacg
     1561 gaagagtaaa cttattgggc accgcaatgg tgcccagtac atttagatat tgtagatcgc
     1621 aaacaataac gagttacgct gtttgtttag aatgcacttc ctgatgctgt ggatacgcat
     1681 atttcggcgc gattgcccct caagccaacg cgatttacgt cggctagcct gacgctgcat
     1741 gcgccagcgc cccacttccg ttctactccg cttcatgtta actactctat gcaataacag
     1801 aatggtcatt atacccccgc ccccgacgct gaccagtgct tttcccgcgt ttttatttgc
     1861 cagatgaatc catatgcgta aactcataac aatgcgcttt caaaaggatt tctaatctat
     1921 gacaaccgtt tatacgttgg tgagttggtt ggccattctg ggatactggt tgctcattgc
     1981 aggcgtaact ttacgcattc taatgaaacg acgcgcagtt ccctccgcga tggcctggct
     2041 gttgattatt tacattctgc cgttagtcgg aattattgcc tatcttgccg ttggcgagct
     2101 ccatttaggc aaacgccgcg ctgagcgcgc cagagcgatg tggccttcca ccgcaaaatg
     2161 gcttaacgac cttaaagcct gtaagcatat cttcgccgaa gaaaatagca gtgtcgctgc
     2221 gccattattc aagctttgcg agcgtcgtca ggggatcgct ggggtcaaag ggaatcagct
     2281 acagctgatg accgagtcag atgatgtgat gcaggcgtta atccgcgaca tccagctcgc
     2341 gcgccataat attgagatgg tgttttatat ctggcagccc ggcggcatgg cggaccaggt
     2401 ggctgaatca ttaatggcgg ctgcacggcg tggtattcat tgccgattga tgctcgactc
     2461 cgccgggagt gtggcttttt tccgcagccc gtggcccgag ctaatgcgta atgccggtat
     2521 tgaagtggtc gaagccttaa aggtcaatct gatgcgtgtg tttttacgcc gtatggacct
     2581 gcgccaacat cgcaagatga tcatgatcga taattacatc gcgtacaccg gcagcatgaa
     2641 tatggtcgat cctcgctact tcaaacaaga tgcgggcgta gggcaatgga ttgatctgat
     2701 ggcgcgtatg gaaggcccca tcgccaccgc gatggggatt atttattcct gcgactggga
     2761 gattgaaacc ggaaaacgta ttctgccgcc accaccagat gtcaatatta tgccgtttga
     2821 acaggccagc ggtcacacca ttcacacaat tgcttctggc cccggctttc cggaagatct
     2881 cattcaccag gcattattga ctgcggctta ttcggcgcat gaatatttga tcatgaccac
     2941 gccctacttt gtgccaagcg atgatttact tcatgcgatt tgcacggcgg cgcagcgcgg
     3001 ggtggatgtc agtattatcc ttccgcgaaa aaatgactcg atgctggtcg gctgggccag
     3061 tcgcgcattc tttacggaac tgcttgctgc tggggttaaa atttatcagt ttgaaggcgg
     3121 gttactgcat accaagagcg tgctggtcga tggcgaacta agtctggttg gcaccgttaa
     3181 ccttgatatg cgtagtctgt ggctgaattt cgagattacc ttggcaatcg acgataaagg
     3241 ttttggtgca gaccttgccg ccgttcagga cgattatatt tcgcgttcac gtctgctcga
     3301 tgcccgttta tggctaaaac gtccattatg gcaacgtgtc gccgagcgac tgttttactt
     3361 cttcagcccg ttgctgtaaa acgtcgccaa cagaggttaa acaggtagtg actatggata
     3421 tggatctaaa caatcgcctg actgaagatg aaacgcttga gcaggcctac gatatttttc
     3481 tcgaactggc tgccgacaat ctcgatccag ctgatgttct gctgttcaat cttcagtttg
     3541 aagagcgcgg cggcgcggag ttattcgatc cggcggaaga ctggcaggaa catgttgatt
     3601 tcgatttgaa cccggacttt tttgccgaag tggtgattgg tctggcggac agtgaagatg
     3661 gtgaaatcaa cgatgttttc gcccgcattc tgttatgccg cgaaaaagat cacaaacttt
     3721 gccatatcat ctggcgggaa taaataatcg cttttagcgt gaaaaagggc tgacaactgt
     3781 cagcccttat tgtttttaaa gcggatcgac tttcaggcaa gaaacggcgt gtctgaaact
     3841 cccctccaga acaggacgtg ttttggcgca ctccggaccg gcaatcgggc aacgggtacg
     3901 gaaaacacaa ccggaaggcg ggttgatcgg cgacggtaat tccccttcca gtaactggat
     3961 ggttttgttc ttctccagat ccggatcagg tatggggact gccgacatca atgccctggt
     4021 gtaaggatgt agtggattgt ggtagacctc atcataggtc cccagttcta ctgcatggcc
     4081 gagatacatc accaacacac gatcggaaat gtgttttacc acggccaagt catgagcaat
     4141 aaaaattaat gacaatccca tctcacgttg cagctgctgg agcaggttaa ccacctgcgc
     4201 ctgaattgac acgtccagcg ccgaaaccgg ctcatcgcag ataatcagct tcggttcaag
     4261 aataagagca cgagcaatcc cgatacgctg gcactgccca ccggagaact catgcggata
     4321 gcggttaatc aggttaggca ataacccgac tttcagcatc atcgccttca cgcgctcgcg
     4381 aacttcctgg cgtgacattt tcggatgata agtacgcagt ggttcagcga tgatctcgcc
     4441 gatggtcata cgcgggttta gcgatgccaa cggatcctgg aaaatcatct gaatatcact
     4501 gcgaacggca cgccattcat cgggcttcat gcccagcaac tctttaccta accaggcaac
     4561 atgaccgtcg gtcgccttga ccaaaccgat gatggcgcga gcaaaggtgg acttaccgca
     4621 tcccgattcc cctaccacac ctaatgtttc cccttcatac aggcgaagag tgacaccatc
     4681 gacggctttg agcgttttcg gcggttgcca gaaccactgt ttgccatctt tgatttcaaa
     4741 gtgcacttta agatcggcga tttcaaggag gacttttctt ccttcagtta cagcattcat
     4801 aacagttcct ccaccggttt aaagcaagca cgcagacggc caggcgtaaa ctcttccagc
     4861 ggcggtgcgc tactacaaat ttccatcgca tgtggacaac gtggctggaa cgggcaacct
     4921 ttcggtaatc gcagcaagtt tggcggatta cccgggatgg tcaacattgt ttcaccttcc
     4981 gcatcaagac gcggcaccgc gttgagcaaa ccgatagaat aaggatgaac gggttgataa
     5041 aagacatcgc gagcattgcc atattccatc gtgcgcccgg cgtacattac cagcactttg
     5101 tcacagatcc ccgccaccac gccaagatcg tgggtgatca taatgatggc agtattaaat
     5161 tcccgcttca gttcattcaa tagcgtcatg atctgcgcct gtacggtgac gtccagcgct
     5221 gtagtgggtt catccgcaat cagcaactta ggtcgacata gcaatgccat cgcaatcatg
     5281 actcgctgac gcatgccgcc agaaaattcg tgcgggtaca ttttcatgcg tttacgtgct
     5341 tccggcattt ttaccgcatc gagcatccgc accgactctt caaacgcctc agctttgctc
     5401 atgttcttat gcagcatcag cacttccatc aactgctcac cgacgcgcat atagggattc
     5461 aacgaagtca ttgggtcctg aaaaatcatt gagatttgtt cagcgcgcag tttattgagt
     5521 tcacgctctg gcaaattgag gatttcacgc ccattgaagg ttgccgatcc gccaatgcgt
     5581 ccgttggcag ccaacaggcc catcaacgca aatgcagttt gcgatttacc cgaaccagac
     5641 tcacctacaa tgcccagcgt ttctccggca cgtagggaaa aatttaaatc attgaccgcc
     5701 gtgacgtcgc cgtccggggt actaaaggtg acacgcaaat ctttcacgtt cagcagtgcg
     5761 tcagcctgtt gttgtgcgag cggcacagtt gcagtttcaa ttacgctcat ggctgcactc
     5821 cttaacgatc tttcgggtcg agggcatcac gcaagccatc gccgataaag ttgaaacaaa
     5881 acagtgtcac cacaaggaat cccgctggga acaacagtaa ccatggagag acttccatcg
     5941 agttcgcgcc atcactcagc aatgcccccc agctgcttaa cggctcttgc gtacccaacc
     6001 ccaggaagct aaggaaagat tcaaagagga tcatgctggg caccagtagc gatgcgtaga
     6061 ccaccaccac accgagtacg ttcggcacaa tgtggcgaat aacaatgccc ggcgtcgata
     6121 caccgccaac ttgtgccgcc tcaataaact ctttgcgctt cagactcagg gtttgcccac
     6181 gcacaatacg agccatatcc agccaggaaa ccatgccaat cgccacgaaa atcagcagga
     6241 tgttttggcc gaaaaaggtc accagcaaaa tgacgaagaa catgaatggg aaggagttga
     6301 ggatttccag cagacgcatc attaccgaat ccactttacc gcccagataa ccggaaagcg
     6361 aaccataaag tgtccccacg accactgcca ccagtgccgc agcaacaccg accatgagtg
     6421 agatacgccc gccaatcgca acgcgcacaa gcaggtcgcg accggatgag tcagtaccaa
     6481 agtagtgacc ggactccata tccggggcgc tggacatcat cgcccagtca gtatcgtcat
     6541 aggcaaactg ggaaagcatc ggtgccagga ttacaaataa cgcgatcagc accagcacta
     6601 tcagactggc aaccgccgca cggttatgca taaaacgtcg acgtgcgtcc tgccacaagc
     6661 tgcgcccttc gacctccagc ttttcactga aattttccag cgtctcgctg tttttcttac
     6721 ttaacatcat cgcgagctcc agtatcagta acggattttc gggtcgataa ccgcatatag
     6781 cacatcgaca atggcattaa acaaaatggt taacgcacca accaggatgg tcaggcttaa
     6841 cactaaggaa tagtcacggt tcaatgcgcc attaacgaac agttgcccaa tccccggcaa
     6901 accataaatg gtttcgatga ccatagaacc ggtaataatg ccgacaaatg cggggcccat
     6961 ataggagagc acgggtaaca gagcaggttt taatgcgtgg cgtaaaatga tccgccgcat
     7021 aggtaaccct ttcgcccggg cagtacgaat aaagttggag tgtaatactt caatcataga
     7081 gccacgggta atacgcgcaa tactggcgat ataggcgagt gacaacgcca ccatcggcaa
     7141 tatcatgaat ttaagcgccc caccattcca gccaccgccc ggcagccaat gcaaaatgat
     7201 cgcaaatatc atgactaata atggcgcaac cacaaaactg gggataacaa ccccggtcat
     7261 tgccagcccc atcacggtat agtcccattt ggtgttttgt tttaatgcgg caataacgcc
     7321 agcactaaca cccagtatta ccgcaaggaa aaatgcggcg gctcccagtt tggcagaaac
     7381 gggaaaactg gatgccacca ggtcattgac cgaataatct ttatatttaa acgatggacc
     7441 gaaatcgcca tgcgccagtt gtttcaggta gctgaaatac tgtgtcatga tcggatcatt
     7501 aagatgatat ttcgcttcga tattggccat cacttctggc ggtaaagtac gttcgccggt
     7561 aaaagggctt cccggcgcga ggcgcatcat aaagaacgaa atagtaataa gaataaatag
     7621 cgtcggaatc gcttccagac aacgacgtag aataaatttt aacattgccc taccttctgg
     7681 cctgtgcctt ctgtaatgcg ataaaaatca gacaccgtgg agcaggacac tcctgcccca
     7741 cgtattgcca ttagtgcttc acaatgtaca tattccgggt ataggtatta tccagcggat
     7801 ctttgccggt atagccacca acccacggtt tcaccagacg cgcattcacg tagtaataaa
     7861 caggaacaat ggccgaatcc ttatccagct gttgttctgc tttagtgtac agagctgtgc
     7921 gctgcgcctc gtcagtcact ttcagcgttt ccgccataat gctgtcaaag gccgggctct
     7981 tataatgcgc ggtattcatc gagctgttcg acagcatggt gttcaggaag gaagttggtt
     8041 cgttgtagtc tgcacaccag cctgcacggg ccacatcaaa agtgccctgg tgacgggtgt
     8101 cgaggaacgt tttccactcc tggttaacca gtttgacgtt taccccaatg tttttcttcc
     8161 acaatgaaga ggcagcaatc gccagctttt tatgcagatc ggaggtgtta taccgcaggt
     8221 tgatggtcaa cggtttgtct gcggtataac ccgcttcagc cagcagtttt ttcgcttctt
     8281 cgttacgttt ttcctggctc cagccaaacc attctggctg agtcaatttt gcgccatcag
     8341 tatacggtgg agtataacca taggcgggca tgtcgccctg cgctttcact ttattaacaa
     8401 tgatatcgcg gtccatacct agtttcagtg cggtacgcac acgcacatcg ttgaatggcg
     8461 gtttctggtt gttaatttcg taatagtaag tgcacaggta tggatcaacg tgaacttcgt
     8521 ccgggatctc ttttttcagc ttctggaaca attcgatcgg catgttgtta tacgtcatgt
     8581 cgatttcacc actacggtag cggttgacat cggtaacttc agaagcaata ggcaaatagg
     8641 ttacctggtt aataacggtt ttcgcgttgt tccagtaggt cgggctgcgt tcaagaacga
     8701 ttcgttcgtt tacgacccaa tcttttaagg tataggcacc gttggtgacg atattaccag
     8761 gctgggtcca tttttcgccg aatttttcga tagcggcttt tggcaccggt gaagttgatg
     8821 ggtgaacaag taatttatag aagtacggaa cgggttcact taaggtgact tctaatgtgt
     8881 gatcatcaat agctttcacg ccgagatcgg taatcggttt tttcccttca agaatttcat
     8941 caataccggc gatatgccca tattgcagat aactggcata cggagaagca gtgttcggat
     9001 caacagaacg ttgccagcta tacacaaagt cttgtgctgt gactggcgtg ccatcagacc
     9061 atttcgcatc tttacgcaaa tggaaggtcc agactttcgc gtctttatta tcccaggatt
     9121 cagcgacgcc aggtgctgga tgaccgtcaa gatcgctgac cagtaagcct tcaaacaggt
     9181 ctcggctgat attagactcc ggaacacctt caattttgtg cggatctaat gactgaactt
     9241 ctgaaccatt gttacgtacc agtgtttgtt tttccgccag tgtgacgcct gcgggtacat
     9301 cagctgccag cgcgacattc cctgccatta gcgcagccag aacgccagct gctactaaac
     9361 ttctcttggt gatgttggtc attgtttttt ggactccctc attataatta ctggtattaa
     9421 ccagcatgtg taatcccgga ttgggaccct gcacaagctc ggagactttc tgctgtcagg
     9481 ttactttatc gttacgtatt actatcaccg actttattta ttctggtgat tcagacgatc
     9541 accttatgtc gattcgtact gtgtctcccc tgtcgagaca cattattctt tcgcgacgct
     9601 ctttaattgt taattattct cattaccaat aaagaatcgt ctggcggttg cccggaaagt
     9661 accaaatgcg gttcttactc gccaatacat tttgcaaaat tgttaagcga ttctctttta
     9721 ttgaccgtat cagaatctat agcttgcatc catgccttaa caaacatctt tcaattcaat
     9781 caaatagaga ttggctaagc atgtctatct tttctgatta cagttccagt agtgaaatgc
     9841 acaataattt aacaattgat tattatttag cactttcctc taccaaaggc agtggaatta
     9901 ctaatatcat ttcaattatt cttcagcaag cccaggatta tgatgtggcg aaaataacat
     9961 gatggtgagg agaaacagtt aaggaaatcc ttataactaa ctgtaatttg agaagaatat
    10021 ttttacctat tccttttgct tttgataact gcgcgggaac aaagtaaaaa taatgacgaa
    10081 gtaaacggag catggcagct ccgtttcatt tgaaaggaat taattaagca ggccggggaa
    10141 aatcccctta ataccagtaa cgataaattc aatccccaat gccatcagca atagccccat
    10201 aatacgcgta atcacgttga tgccggtctg gcgtaaaacc cgtaccagcc acggtgccat
    10261 gcggaacaat ccccagcaac ataaagcgaa caatgcaata gccacaaaga aaccaaacag
    10321 atagctaatg ctgtgataac gcgtacccca gacgatggta gaactgatcg cccctggccc
    10381 cgccatcaac ggcaacgcca atggcaccac accaatactt tcacgtaccg cggtttctga
    10441 tttttcttgc ttgttctgtt tatcctcgcc aagcttgccg ctgatcatcg acatcgctat
    10501 cgtcaccacc aggatgcccc cggcgatacg gaacgaatca attgatatac caaaaagttg
    10561 tagaatcgtg tcgccgagaa aaagcgagat ccacaagata atggccacag acaggttggc
    10621 tgtaaggtta gttttgtttc gcgctgccgc tgtctgataa ctggtcatgc tgataaagac
    10681 gggaataatc cctaccgggt tgaccagcgc aaataacccg atgaaaaatt tgaagtaaac
    10741 gggaaaatca aaaagggtct gaatcacggt tcgctccgaa gcaaaagccg gataatgtta
    10801 gccataaata aggttgaaaa gacgcgctga caatacgcct tttgacagca tttttcacct
    10861 cctaactact taaaattgct atcattcgtt attgttatct agttgtgcaa aacatgctaa
    10921 tgtagccacc aaatcatact acaatttatt aactgttagc tataatggcg aaaagcgatg
    10981 ctgaaaggtg tcagctttgc aaaaatttga tttggatcac gtaatcagta cccagaagtg
    11041 agtaatcttg cttacgccac ctggaagtga cgcattagag ataataactc taatgtttaa
    11101 actcttttag taaatcacag tgagtgtgag cgcgagtaag cttttgattt tcatcggtta
    11161 agcaaatcat caccgcactg actatactct cgtattcgag cagatgattt actaaaaaag
    11221 tttaacatta tcaggagagc attatggctg ttactaatgt cgctgaactt aacgcactcg
    11281 tagagcgtgt aaaaaaagcc cagcgtgaat atgccagttt cactcaagag caagtagaca
    11341 aaatcttccg cgccgccgct ctggctgctg cagatgctcg aatcccactc gcgaaaatgg
    11401 ccgttgccga atccggcatg ggtatcgtcg aagataaagt gatcaaaaac cactttgctt
    11461 ctgaatatat ctacaacgcc tataaagatg aaaaaacctg tggtgttctg tctgaagacg
    11521 acacttttgg taccatcact atcgctgaac caatcggtat tatttgcggt atcgttccga
    11581 ccactaaccc gacttcaact gctatcttca aatcgctgat cagcctgaag acccgtaacg
    11641 ccattatctt ctccccgcac ccgcgtgcaa aagatgcaac caacaaagcg gctgatatcg
    11701 ttctgcaggc tgctatcgct gccggtgctc cgaaagatct gatcggctgg atcgatcaac
    11761 cttctgttga actgtctaac gcactgatgc accacccaga catcaacctg atcctcgcga
    11821 ctggtggtcc gggcatggtt aaagccgcat acagctccgg taaaccagct atcggtgtag
    11881 gcgcgggtaa cactccagtt gttattgatg aaactgctga tatcaaacgt gcagttgcat
    11941 ctgtactgat gtccaaaacc ttcgacaacg gcgtaatctg tgcttctgaa cagtctgttg
    12001 ttgttgttga ctctgtttat gacgcagtac gtgaacgttt cgcaacccac ggcggctatc
    12061 tgttgcaggg taaagagctg aaagctgttc aggatgttat cctgaaaaat ggtgcgctga
    12121 acgcggctat cgttggtcag ccagcctata aaattgctga actggcaggc ttctctgtac
    12181 cagaaaacac caagattctg atcggtgaag tgaccgttgt tgatgaaagc gaaccgttcg
    12241 cacatgaaaa actgtccccg actctggcaa tgtaccgcgc taaagatttc gaagacgcgg
    12301 tagaaaaagc agagaaactg gttgctatgg gcggtatcgg tcatacctct tgcctgtaca
    12361 ctgaccagga taaccaaccg gctcgcgttt cttacttcgg tcagaaaatg aaaacagctc
    12421 gtatcctgat taacaccccg gcttctcagg gtggtatcgg tgacctgtat aacttcaaac
    12481 tcgcaccttc cctgactctg ggttgtggtt cctggggtgg taactccatc tctgaaaacg
    12541 ttggtccgaa acacctgatc aacaagaaaa ccgttgctaa gcgagctgaa aacatgttgt
    12601 ggcacaaact tccgaaatct atctacttcc gccgtggctc cctgccaatc gcgctggatg
    12661 aagtgattac tgatggccac aaacgtgcgc tcatcgtgac tgaccgcttc ctgttcaaca
    12721 atggttatgc tgatcagatc acttccgtac tgaaagcagc aggcgttgaa actgaagtct
    12781 tcttcgaagt agaagcagac ccgaccctga gcatcgttcg taaaggtgca gaactggcaa
    12841 actccttcaa accagacgtg attatcgcgc tgggtggtgg ttccccgatg gacgccgcga
    12901 agatcatgtg ggttatgtac gaacatccgg aaactcactt cgaagagctg gcgctgcgct
    12961 ttatggatat ccgtaaacgt atctacaagt tcccgaaaat gggcgtgaaa gcgaaaatga
    13021 tcgctgtcac caccacttct ggtacaggtt ctgaagtcac tccgtttgcg gttgtaactg
    13081 acgacgctac tggtcagaaa tacccgctgg cggactatgc gctgaccccg gatatggcga
    13141 ttgtcgacgc caacctggtt atggacatgc cgaagtccct gtgtgctttc ggtggtctgg
    13201 acgcagtaac tcacgccatg gaagcttatg tttctgtact ggcatctgag ttctctgatg
    13261 gtcaggctct gcaggcactg aaactgctga aagaatatct gccagcgtcc taccacgaag
    13321 ggtctaaaaa tccggtagcg cgtgaacgtg ttcacagtgc agcaactatc gcgggtatcg
    13381 catttgcgaa cgccttcctg ggtgtatgtc actcaatggc gcacaaactg ggttcccagt
    13441 tccatattcc gcacggtctg gcaaacgccc tgctgatttg taacgttatt cgctacaatg
    13501 cgaacgacaa cccgaccaag cagactgcat tcagccagta tgaccgtccg caggctcgcc
    13561 gtcgttatgc tgaaattgcc gaccacctgg gtctgagcgc accgggcgac cgtactgctg
    13621 ctaagatcga gaaactgctg gcatggctgg aaacgctgaa agctgaactg ggtattccga
    13681 aatctatccg tgaagctggc gttcaggaag cagacttcct ggcgaacgtg gataaactgt
    13741 ctgaagatgc attcgatgac cagtgcaccg gcgctaaccc gcgttacccg ctgatctccg
    13801 agctgaaaca gattctgctg gatacctact acggtcgtga ttatgtagaa ggtgaaactg
    13861 cagcgaaaaa agaagccgct ccggctaaag ctgagaaaaa agcgaaaaaa tccgcttaat
    13921 cagtagcgct gtctggcaat ataaacggcc ccttc
//
LOCUS       DAJTQY010000098        13798 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.098, whole genome shotgun sequence.
ACCESSION   DAJTQY010000098 DAJTQY010000000
VERSION     DAJTQY010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13798
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.098"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(<1..816)
                     /gene="wcaD"
                     /locus_tag="OO349_003158"
     CDS             complement(<1..816)
                     /gene="wcaD"
                     /locus_tag="OO349_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HCR5868139.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISIKKLMIALGIGAGLTAFNYLFGQSLDAGKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDALILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFRVGVVIAFPYISTRLGDLG"
     gene            complement(791..2008)
                     /gene="wcaC"
                     /locus_tag="OO349_003159"
     CDS             complement(791..2008)
                     /gene="wcaC"
                     /locus_tag="OO349_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HCR5868140.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLNSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREILALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(2005..2493)
                     /gene="wcaB"
                     /locus_tag="OO349_003160"
     CDS             complement(2005..2493)
                     /gene="wcaB"
                     /locus_tag="OO349_003160"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HCR5868141.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(2496..3335)
                     /gene="wcaA"
                     /locus_tag="OO349_003161"
     CDS             complement(2496..3335)
                     /gene="wcaA"
                     /locus_tag="OO349_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HCR5868142.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            complement(3428..5590)
                     /gene="wzc"
                     /locus_tag="OO349_003162"
     CDS             complement(3428..5590)
                     /gene="wzc"
                     /locus_tag="OO349_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HCR5868143.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITVVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEEEKAKLNGRVTAMPKNQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIIDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGVKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(5593..6036)
                     /gene="wzb"
                     /locus_tag="OO349_003163"
     CDS             complement(5593..6036)
                     /gene="wzb"
                     /locus_tag="OO349_003163"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HCR5868144.1"
                     /translation="MFNNILVVCVGNICRSPMAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMDKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(6042..7181)
                     /gene="wza"
                     /locus_tag="OO349_003164"
     CDS             complement(6042..7181)
                     /gene="wza"
                     /locus_tag="OO349_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HCR5868145.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALANAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            7490..7639
                     /locus_tag="OO349_003165"
     CDS             7490..7639
                     /locus_tag="OO349_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5868146.1"
                     /translation="MVIEYWLANNAGGSVTANGIAGKVTIRQLAIFKNYIKYHSIWFL
                     GVSLG"
     gene            7840..9423
                     /locus_tag="OO349_003166"
     CDS             7840..9423
                     /locus_tag="OO349_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCR5868147.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAKTASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            complement(9875..11728)
                     /gene="asmA"
                     /locus_tag="OO349_003167"
     CDS             complement(9875..11728)
                     /gene="asmA"
                     /locus_tag="OO349_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HCR5868148.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQSQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGKVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(11750..12331)
                     /gene="dcd"
                     /locus_tag="OO349_003168"
     CDS             complement(11750..12331)
                     /gene="dcd"
                     /locus_tag="OO349_003168"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HCR5868149.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(12423..13064)
                     /gene="udk"
                     /locus_tag="OO349_003169"
     CDS             complement(12423..13064)
                     /gene="udk"
                     /locus_tag="OO349_003169"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HCR5868150.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            13382..>13798
                     /locus_tag="OO349_003170"
     CDS             13382..>13798
                     /locus_tag="OO349_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HCR5868151.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLVSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLAFGSAIVPPLLG"
BASE COUNT         3193 a         3640 c         3494 g         3471 t
ORIGIN      
        1 acctaaatcg cccagacggg tggaaatata aggaaacgcg attaccacac caacccggaa
       61 tacagccaga gaaatcaatg ctaacggtaa ttttttctta atcgcctctt tattcagata
      121 ttgaaatgcc cactccagca aataaaacag gataaaggtc ataacccctg aaaacgatcc
      181 ggataatatt atccctgcga gaatcagggc atctgtttta ggcgttttga taccaaactg
      241 tttgatgctg agccaaattg agattaatgc cagagcgaaa aatgccggtt cgaaataaag
      301 tgctgtcgtg cgcttgccgc cgaatttaat gaaattcagc acatagctgt tgctgtaaat
      361 cagatatttc gaaatcgact ccataatact gctgccaccg gtgaggataa tttgcgccat
      421 ctccaccgcc gccagcgcca ccaccagtcc gaccaccaga taaaagaaac gtaatatctt
      481 acgatggttg tgtggcgaaa ttgttttaaa acgaatactc cacaccatgc caataatgat
      541 cacaatatag acaaacagca tagttgaagt gacgtattta ccggcatcca acgactgacc
      601 aaacaggtag ttaaacgccg tgagtcccgc gccaatccct aaggcaatca tcaatttttt
      661 aatgctgatg cgttctaaaa acagcagtaa caagacaggt aaaaaagtga cgatggtgat
      721 ggggaaactt tcgccaagct gggcgatttt gacgttaacc agcaggtaga tcagtggcag
      781 cagcaggtag ctacagattc tgatagaagt tgacatactc ctccagcatc tgttgtccac
      841 tgtaggcggc gcgactgcgt tggctgaact cagccagcgt ggtaccaaat atcgcctgcg
      901 cgatttccgg tttgcttaac tgtaccagtt gcagcacctc ttcttcactg acggttttac
      961 caccggattt ttgcagcact tcccgcgccg catcgctatg ggtggcaatc accggcacgc
     1021 caatcgatag cgcctcacac aaaatcagcg ggtagttatc gacacgagaa ctgaacacca
     1081 gcgcatccat ctgattgagc gcgctcatca acttgcgctt gtcagtttca aagccgtgat
     1141 taaccacgtt gccagcggtg aacggcgaga acttaccaaa ggtatgcagt tcgattttgt
     1201 cgcccagcgc catcatctca cgcaccagtt gctggttagt tttgccgtcg taacgcaggt
     1261 catgcgccac cactgcgatt ttcggcttgc cctgagtttc gcgcaccgga ggcagatctg
     1321 ccagaatcgc ttcggttgcc atatcaatgc cattattgat aatccggcaa cgccctggcc
     1381 cgtacagact attgaaagcg tcagccacat gctggctggg ggaaataaac tgacagccca
     1441 gcgccagtat ctcgcggaat aactggcgtt tgcccgccac cagctggtgc gcgcgatcaa
     1501 tcttcaccgg cggatagtta tttaaggtcg ggcatttctg gcagcccgtt ttccagcctt
     1561 cgcaaccgtc ggtaaaggcg cagcgtccgg ttacactcca gtggtcgtgc agcgtccaga
     1621 ccagggtgac atccggttta tggtttttca ctttttcgca aaagcgcacc acgctgttaa
     1681 gattcaacca gtagctgtgc agcacatgaa aatgcaggac caccggaccc ggtgtgcgag
     1741 taatggtgcg atataactca ttgaaattgc cgaacagatc gcgattaaac agacgaaaca
     1801 acgcaatgtt cgccatcgcg gtcatccgcg gcgtatgttt gatgacctgc ggatagttct
     1861 gatggctgac gctctctttg ccgcctttgc cgtagccgta gacaaaatgt gacgccagcc
     1921 cctgttgcag cgcacgctgg tggagatcta acgccacacc tgctgccccg ccttccgcca
     1981 gtcgcacatt aaattgcaga atattcattt aatcaccttc actcgcgctt tttctcccac
     2041 caccagcgcg ttgtccggga cagagtcgag caccacgctg cccgcaccca cggtgacgtt
     2101 gttaccaagc gtgatatcac caagaataat gacgttggca ccgagttcga cgccgttgcc
     2161 aatgtgtgga catgccatgt tatcggcacc ccgattgccg atagtgacgc cgtggcgaat
     2221 ggtgaaatca tcccccgcca ccacgttttt attgatcact acggcgtaac cgtgatggat
     2281 agtaaagcgg cggccaatgg tcgcggcggc ctggatttca taaccgaaaa agcattcggt
     2341 gataatgcga tacagcacca gcagcggggc cgcccacaga ttgttgagga cgttcttttt
     2401 gcgccacacc gaacaaaaat gagcgacgcg ataggcaagt accatgcagc acgggcgtaa
     2461 actccagctg ttggcgcgca gatcttccag catctttagc gcccccgaat accatcagcc
     2521 agacgtttgc cgttacgcac cgacagcagc gtcaacaagg tgcgccaggt catacgttta
     2581 ttgcggatct ggtagagggt aaacagctga tattttttac tggcgcggtc gaatttgtct
     2641 ttgtgcttgc ggtaaaagtg gaagtatccg gagaattttt tcggtgacga ggtgatctgc
     2701 atttcgccgt gattgatatg caggatctgc gtcgcctctt ccactttcca cggttcgccg
     2761 tactccacca ccatccgcag gaatatgtcg taatcctgtg ctgctttcag ttcggtatcg
     2821 aacaaacact ctttgaaacg ccatgcccag gtaaagacct gattgccaat gatattacgt
     2881 ttatagaaca ggcggcgtga gtacggcgat ttgggataca gtggtaagct cgccggttgg
     2941 gaataaactt ctccctggca aacgtagtcg ttagcgtaca aaaaggcgtg tgtgaccagt
     3001 tgctgtttat gggcgaggaa gacgctcagg cggttgggcg tccattcatc atcatcatcg
     3061 atcccggtga tgtattcccc ttgcgccagc ataatcgcct ggttacgcac cgcgcacgcc
     3121 ccgctgttaa tgtcgttgtg gatgtaagtg atacgcggat cgttgagggc agtgacgtac
     3181 tgttgcagtt gttcccagga ggtggagcaa tcatccacga tgatcatctc ccagttgctg
     3241 tagtcctggc gcaggaccga ttttatcgcc cgaatcgcca gttgttggcg gttccaggtt
     3301 ggcatataga ttgagatcag cggattgtct ttcataggtt gttctcccgg cttatgagct
     3361 ggtttgcttg ccggatgcgg cgtgagcgcc ttatccggcc tacggggcgg tgcgaatgca
     3421 ggcgtcgtta tttcgcatcc gacttatatt cgtattcgta atagccataa tcctgatacg
     3481 cgctggcgcg gcggaagatg gagttcagaa tcaccccttt caccggaata ccgttttgct
     3541 caaagcggct cagactggtt tccacttctt tcaatgtgtt gaccgcataa cgcgccacca
     3601 ttaacgtggt tccgacatga cgaccaacaa ttgccgcatc ggtcactgcc agaatcggcg
     3661 gcgtatcaat caacaccagg tcatagtttt tactcgccca gttcaccagt tcggcaaagc
     3721 gttcgctcat taacagttca gaaggatttg gcggcacctg accgcgcggg atcaggtcaa
     3781 atttggcaat agaggtcggt ttagcagcgg tagtaatatc gccctgacca atcagaatct
     3841 ccgacagccc attgacatta ttggtgccca acagctcgtg ggtgtagcct ttgcgcatat
     3901 cgcagtcgat caacaacacg cgtttattgg tctggctgat caccgccgcc aggttggcgc
     3961 agacaaaggt tttaccgatt gacgggctaa ccccggtcat catcaacaca ttgttctgcg
     4021 cctgcatcat cgcgaagtgc agactggtac gcagactacg gatggcttca atcgccagat
     4081 cggttggatt ccccactgcc agtagctggc tctgtttata gcgtttaacc cctttgatgg
     4141 ttttgacgct atcgcgcgct ttctgccact ccgacagcgg gatgctggca tagacgctga
     4201 ttccgtgttc ttccagcact tgcgggcttt cgatgccgcg attaaacaac gagcgcagca
     4261 gcacacccac gatagagagc atcaggccaa ggataatcgc gccgaggata atcagccctt
     4321 tcttcggttt tagcacgcca ggctgagtga ttgccgggtc aataatgcgc acatcgccga
     4381 cggtgctggc ttcggtgatt ttcagctcct gctctttatt cagcagttgc atatagacct
     4441 gctgaccaga ctcgacatcg cgggtcagac ggacaatttc ctgctggttt ttcggcatcg
     4501 ccgttacgcg accgttaagt ttggctttct cttcttccag cgcctgacgt ttctccagca
     4561 gcgtgcggta cgccgggtga actttggtgt acagcttgga gatttccgcc tctttaaagg
     4621 tcagttcgtt caactgcgcg tcgatgttca ccatcgaatc gagcaccgct ttcgcttcaa
     4681 gcggcagatc aacagaatct ttatcctgac ggaaggcatt cagtttgttt tcggcaacat
     4741 cgaggcggct acgtacttcc ggtaactgtt gcgcgaggaa agcgaggctt ttcgacgctt
     4801 ccgccgattt gcgctcaata ttttgttcct gatagttacg agcgatgctg ttaagaatgt
     4861 cgcggatctg ttcgcgatct tcaccggtat aagtcaggct cagtacgcct gcgtctttgc
     4921 cgttctccgt taccgtcagg ctgttttgca gttggttgat catccccaac gtggagtatt
     4981 tggtgacggt aaactcactg cccgggcggg cgtgaatggc ttcaaccatc agcgtgacgc
     5041 cttctttttt cagcatctgg cccgcttgcc cacgggcgct aaagccgcca tcactgctca
     5101 gggtgtagtt tttgtcatcc agcacattaa gcgtaaacac ctgatccgcc atctctttcg
     5161 ggcggttaaa ggtagtcact ttcaccgtct cgttctgacg ccccatcagg cgatcccagc
     5221 ccgccccgaa aatcgggaac gtgtttttgc tcaccgcaat atcgaggtcg agatcgtcca
     5281 ccgttttacc aagcaccaga cgcgaacgaa tcaactggat ctcggcgtcc gatgcaggcg
     5341 gtttgttggc taatgccgag ccgatatcct gcaccagcga attgccgctg ttttgctcga
     5401 tttgtaccag tgcgtcggca ctataaatcg gcgtggcgaa gaaagtgtaa accacggcac
     5461 agagggcaaa tacgacggtg atgccaatca cccaccagcg cgcttcaatg acggtgccga
     5521 ccaggcgacc aatatcgatt tcatcactgc ccgttaccgg agcggcatgt tgttttactt
     5581 tttctgtcat tcttatacct gctctgcgtt caatgcctgc gcccactggc gggcagaccg
     5641 ttcaagtaat gtgtacaccg ctgcaaacgt ttcccggctt ttgcgatacg gatcggggat
     5701 ttcacattcg ttatcccagt gaccaaacag catcactttg ccgcgcatct ccggtgccat
     5761 ctcgcataag cgttcgatat ggcgcttatc catggtcaaa atcaggtcat agttgcgaca
     5821 caggcggcgg ctgatttgtc gtgcacagtg gccttccaga gacagttgat gttctgcggc
     5881 gacgctgata gcggtaggat cagcgccctt accgaccagc gcgccgagtc cagcggactc
     5941 cactttcagt tccgggtgat aacgttgcag taagcgttcc gccatcgggg aacggcaaat
     6001 attgccgaca cagacaacta agatgttgtt aaacatgacg attaccagtt atgaatgtcg
     6061 ctggctgtat ccgtcatgta acggacaccg ctaatagttg gcagcaactg attgatcaga
     6121 cggttccagc gggaaaccgg ggcggtggtg acatacacca cgtcataagg ttgcaggcgg
     6181 aattctgtcg ccatcaccag cgacgtcgca tcggacatat ccagctggta gatattggca
     6241 atcttgccgt tacgcccgcc ctcgcctttc agcggacgaa tgacaaagat gccgctggcg
     6301 ttggaggtgg tcatgtcgat accttcggca ttggccagag cttcagtcag ggtcatgccg
     6361 ctaaagtcca ttttcagggt gctctgtttc ttcacttcac ccatcacaaa tactttcaga
     6421 tcatcattac gcggcacgta gagaatatcg ccgggataaa gcagtcggtt ctgattgagg
     6481 tcgccgtttt gcatcagcgc ctgtaaagaa atgcgttctt cacgaccatt gtgtgttagc
     6541 accacgttgc gccagtcagc ggtgtcggtc aggccacctg cggcgttgat ggcgtcgaga
     6601 atggtcagtg gtacgttggt gatcgcctgt tgaccggatt tattcacctg acctgagata
     6661 taggcctttt gtgagcggaa ggcggcgata ttaacgtcca cctgcgggtc agcgatgtac
     6721 gtcgctaagc gcccggtaat atcactgcgg atttcagcga gcgttttccc gactacgtga
     6781 accttgccga tatacgggta aaacatagtg ccgtcaggct gtacccagtt gccggtgtcg
     6841 ctggagctgc ggtactgacc agctggcgtg gtgagttccg ggtgatccca gacggtgaca
     6901 ttaagaacgt cccccggccc gacgcgatac tggtaattcg cgatctcgct ttccagcgtc
     6961 atattggggc gcgctacatt cgggcgtggg cgtaattggt caatcaggcg cggggtcagc
     7021 ggataaacat tcaccatttt gtcgagatcg aaatcagcgt cctgctgttt gatgacgtct
     7081 ttgcccatcg tcgacatatt gctgcccgga agtactgtgc aaccgcttat caaggttact
     7141 gacaccaata atggcatcaa tttcattttg gatttcatca ttgtttattt atcactttgg
     7201 cagagtaatt atcctgtgca ctattaatag caatgtcgcc atgcacattt accttgcagt
     7261 taattgaata aaaatttaac tggcatcagt cctaaaaaaa ttgatttcat ccgcaggcta
     7321 ttgacagaat aattcagact ggtctttcag gcatccagac acgctaccgc ccctggcttt
     7381 ttagctacca atacactgat tgagcttaat ttttcaaacc ctctctgcat acaatcgttg
     7441 atgagaaagg gttattgcgg aaattaactt ccgaatataa ggtgacatta tggtaattga
     7501 atattggctc gccaataatg caggaggaag tgttacagct aacggaatag caggcaaggt
     7561 aacaattcgg caattggcta tttttaagaa ttatattaag tatcattcaa tatggttttt
     7621 aggagtttct ttaggttgac aatatttaat attgcgtctc cacatgcgat atttcttaaa
     7681 taatgtttta ttattaccgc ttttaattca gggataatgt gaggttatta ccctcaaata
     7741 atatgagata aatagtgctg caacattgca tttttgcccc gatttatcca tgatcgaatt
     7801 gtgacatttg tcatacaacg aataggtttt gtacttacta tggaatggat tgccgatccg
     7861 tctatctggg ccgggttaat cacgctgatt gtgatcgaac tggtcctcgg cattgataac
     7921 ctggtcttta ttgccatcct cgccgaaaaa ctaccgccga agcagcgcga ccgcgcacgg
     7981 gttaccgggc tgctgctggc gatgttaatg cgcctgttac tgctagcgtc aatctcctgg
     8041 ctggtcaccc tgactcaacc gctgttcagc ttccgctcgt ttacctttag cgcccgcgac
     8101 ttaatcatgc tgtttggtgg tttcttcctg ttgttcaaag ccacgatgga gctgaacgaa
     8161 cggctggaag ggaaagacag caataatccc acacaacgca aaggtgcgaa gttctggggc
     8221 gtggtgacgc aaattgtggt actggacgcc attttctcac tcgactcagt gattaccgcc
     8281 gtcgggatgg tcgaccattt actggtcatg atggccgccg tggttatcgc tatcagcctg
     8341 atgttgatgg ccagcaagcc gttaacgcaa tttgttaaca gtcacccgac catcgttatt
     8401 ctctgcttaa gcttcctgtt gatgattggc tttagcctgg tggcagaagg tttcggcttc
     8461 gtcattccga aaggctacct gtacgctgcc attggtttct cggtgatgat cgaggcactc
     8521 aatcagttgg ctatctttaa ccggcgacgt tttctttccg ctaatcagac gctacgccag
     8581 cggactaccg aagcggtaat gcgcctgctt agcgggcaaa aagaagatgc ggaactggac
     8641 gccaaaaccg cgtccatgct ggtggatcat ggaaaccagc agatctttaa tccgcaggaa
     8701 cggcggatga ttgagcgggt acttaatctt aaccagcgta ccgtaagcag cattatgacg
     8761 tcgcgccacg atattgagca tatcgatctc aacgcgccgg aagaagaaat ccgccagttg
     8821 ctggagcgaa atcagcatac gcggctggtg gttaccgacg gtgatgacgc agaagatttg
     8881 ctcggtgttg ttcacgttat cgacctgcta caacagtcac tgcgcggcga accgctcaac
     8941 ctgcgggtgt tgattcgcca gccgctggtg ttcccggaaa ccttgccgtt gttaccagcc
     9001 ctggagcagt tccgtaatgc ccgcacgcac tttgcttttg tggtggatga gtttggctcg
     9061 gtggaaggga ttgtgacatt aagtgacgtc actgaaacca ttgccggtaa cttaccgaac
     9121 gaagtggaag agatcgacgc ccgccatgat attcagaaga atgcggacgg ttcctggacg
     9181 gcgaatggtc atatgccgct ggaggatctg gtgcaatatg tgccgctgcc gctggatgaa
     9241 aaacgtgaat atcacaccat tgccgggctg ttgatggagt atttgcagcg tattccaaaa
     9301 cctggcgaag aagttcaggt gggggattat ttgcttaaaa cgttgcaggt ggaaagccat
     9361 cgcgtgcaga aggtgcagat tataccgctg cgtaaggatg gcgagatgga gtacgaggtg
     9421 tgatggtatc ggccatttta cccaatgctg cgtaacgcct tataacttca gcaatgaagc
     9481 gttgtctgtg gtaaacggac atcacagcgg gggtgtgggt ccggctgtaa atcgccgaat
     9541 cgtttcctgt aggctggggc agcccacatg gatgtgggct gagggcgcgt tttacaggga
     9601 tgttacctcg cgcccgtacc cgatagccga aaggaataag atgagggaac cgcgccagcg
     9661 gcgatttacc gccgggagcc cgggttgcca gggtggtggc gattgagcca ccctggcacg
     9721 ttcaccggct tgagcgttcc atagtagcga ggaacatcaa gtgaacggaa cagccttcga
     9781 tcttacatat tcagaatata atagtgccgc cgcgaacgcc tgatgcgacg ctttacgcgt
     9841 cttatcaggc ctacaggaca ggtcacctgg taacctacat cttctccagc aacttcttca
     9901 catctttgcc attgcgggaa tctttattcc gctcggccca gtcattcagg cgacgtttcg
     9961 cttcgtcctg tagatgtttg cgcagtaact gatccacttg caggctgtaa ttgagttgct
    10021 gccagttgcc ataaacccgc agcggtactg gcgtttcttt gaggaaatca atcagtttgc
    10081 tttccccgtt ccagccaccc acgacccgaa tatcaaactg ggtgtcgcag gtttgatctg
    10141 ccagattcaa catgccttcc cctgtcagcg ccagcacagg cgattgacct tgcatgtcgt
    10201 ttaacgtcac gacgccatcc ttcaacgtca aatcggtggt aaagcggtca agccgcgtca
    10261 cgttatcgaa gttttcagcg gccttcacat caccgccatt acgctctacc gcttgctgaa
    10321 tcatctgctg gaagttcatc ccttccatac gtgtgtcggt catctcaaca tgcgcctgtc
    10381 cttgccagtt gtggcggaat gcgtcggcat ctatgtcagc accggagaaa tcaccagcca
    10441 gtgacatttt tccggtcaac gaaatcggat agttaaacgc cttcagaatg gtaccaatct
    10501 caacgttctc cagccgtggc tggaagttta tccgcggatt tattgatgtc gcgtccagcg
    10561 tgcccggcag tgaaaccttt ccaccgttaa gcttgccctg cagttgagta atttccagca
    10621 aacccgactt gttggtcatt tgcgtggcaa catctgtaaa attcattccg cgccagcgca
    10681 cgttactggc ctgcaacaaa atatcagctg taaagccttg cagtccctga taggccggtt
    10741 catcaatacg cgaagaaatg accgggcgcg gcaacgttga ttggctctgc ccctgctgcg
    10801 cggcaccgtt ttcaccattc gcggtttcat taagcgggat gaggttgtca agattcagtt
    10861 gcgggaattg cagtctcagc tgccattccg gtttctctgt cagcgtgacc tgtgcttgtc
    10921 cgctcagcgt actgtcattg gcggtcaaac taatttggtt aaatgaaagg cgtttatgtg
    10981 actcctgcca ctgggactgg aaactcccct gcccctgaat accttgtttt ggtaaatcgg
    11041 caccctgcaa ctgccagtta atttgttcaa tagccgctgt taaatcatgc ggataatcag
    11101 aagcatctac cgtaccgtta agggatatcg tgagatcgcg ctgatcgcga ttaacccgcc
    11161 cggagaactc aaatgagcca cgatgttggg gatcttgttc catttgcagg cggatattgc
    11221 ggattgtcac ctgctcgtca tcttcatgct ggaacaccag aacgctgtcc gccaccttaa
    11281 gactggatat atcaaacgac catccgcgat cgtctgacag atccggcaag gtattgtcgc
    11341 gcggtgcaac cggagcgtct tcactgcgca ccgcttccgt ctgcggcgtc agttggatca
    11401 ctgccccttt tagcatcacc tgcttaacgc tcagttgatg actcagtagt ggtaaaagcg
    11461 ccacgtccag acgcatgttg tcggcgcgaa ccagtggctg gcttgcgccc tgggcggtga
    11521 gagacattcg cccggagagg atactaagct gcggccagac gtgccaacgc agtggcccgt
    11581 cgagctgcaa ttgataaccg ctacgtgcag caacttgctt gaccatatag tcgcggaaat
    11641 cattcggatt caccagcaac actaacgcag ataacccggc caccagcacg accaagagta
    11701 tcatcagcgt cgtcagaaat cgtctcatgg tatcctcaat gggcctgaat tagtctttat
    11761 cgattcggct ggctaccgcg ccctgctggt tgcgatattt cgcatcttca cggcggttgt
    11821 aaggtcgcgc cgccgggccg gaaagcggct caaagctcag cgcaccaatt aacatgcccg
    11881 gacgcagcgc cagcggcagc ttaccggagt tgtagaactc cagcacaatg caaccagacc
    11941 agcccggatc gatacggtgc gcggtgacgt gcaccatcag ccccagacgc gccaaagagg
    12001 aacgcccgtc cagccagccc accagatcgg ctggcagcgt caccgactcc agtgtcaccg
    12061 ccagcgccag ctctcctggg tgaagataga acgcctcgcc ctcgtcgaga acgatctcat
    12121 cgctcatcac gcggtcaagc gcggcgctca cttcatcttt gggaccgctc agatcgataa
    12181 acgctgccgt gtgaccacgg aaggtacgaa atttattgcc caggcgtaca tccaccgtcg
    12241 cgccgttaat acgctccact ggcggacgtg ggttgatcga caaacggcct tcatcaagcc
    12301 aggcttcaat atctcggtca cacagacgca tggcattttc tcctttcgca catcactccc
    12361 ttaacgccaa ttacgtcaag ggcataccaa cttatcactg aacggtacac aatttatcaa
    12421 gcttattcaa agaactgact tattttcgct ttcaatatat cgatcgcgat gcggtttttc
    12481 ccgccgcgcg gcacgataat gtccgcatat tgtttagaag gctcaatgaa ttgcaggaac
    12541 atcgggcgca cggttttttg atattgcgcc atcacggaat ccattgaacg cccacgctcg
    12601 ttaacgtcac gcttgatgcg gcgcatcagg cagatatcca gcggggtatc aacgaaaatg
    12661 gagaagttaa gttcgtcacg caaacgcgca tccgtcagca acaaaatgcc ttcgagaatg
    12721 atgaccttct tcggctcaac cgtcaccgtt tctttcatac gcgtatgttc aacatagctg
    12781 taaaccggca ggtcaattgc cgagccgcgt ttcaacgctt gtaaatgctc aagcaacagg
    12841 ctgtgatcca tcgcgctggg atggtcgtag ttggtcttaa cgcgttcttc catcgacaga
    12901 tggctttgat ctttgtaata gcagtcttcg ggaattacgc cgatgtgttc atcaccgact
    12961 tgctcacgta attcacgata aagggtactg gcaataagac tcttgccgga agccgatgcg
    13021 ccagcgatac cgataatgac gcactgatga gactgatcag tcatatattt agcgacctga
    13081 ttaacctgga tgttaggaag ggggcgacga agcgccaaac gcggcaatta tagggatttc
    13141 atccgtctga taccagtcga atagcgttgc cgcgcgctca gagttaattg ttgacaaaga
    13201 attcccggtg gcaaattacg ttgatcagtt ttatgtaagg taaaaaatgt tatacgcagt
    13261 tgcgcaaatt atccgccttt acgtcacttt atgagcaatt cgcatataaa atgtaaaact
    13321 tttgtactag cataaacaca gaaacgaata ctggcgacct ggccttgcgg ataaagcggt
    13381 aataagcaaa caatcacagc atgtattaat tgccctgccc cacccgctgc ttcacctggt
    13441 cagtttaggt ttagtctcgt ttatctttac ccttgtctcg cttgagcttt cgcagtttgg
    13501 cacccaactc gccccactgt ggttcccgac gtccatcatg atggtggcgt tttatcgcca
    13561 tgccgggcgc atgtggccgg gaattgcgct gagctgttcg ctgggaaata tcgccgcatc
    13621 catcctgctt ttttccacca gctcgctgaa catgacctgg acgaccatca atattgttga
    13681 agccgtggtc ggggcagtgc tgctacgtaa attgctgccg tggtataacc ccttgcaaaa
    13741 tctggctgac tggctgcgtc tggcattcgg cagcgccatt gttccgcctc tgttgggg
//
LOCUS       DAJTQY010000099        13535 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.099, whole genome shotgun sequence.
ACCESSION   DAJTQY010000099 DAJTQY010000000
VERSION     DAJTQY010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13535
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.099"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(<1..893)
                     /locus_tag="OO349_003171"
     CDS             complement(<1..893)
                     /locus_tag="OO349_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5868152.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPG"
     gene            1134..1934
                     /locus_tag="OO349_003172"
     CDS             1134..1934
                     /locus_tag="OO349_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCR5868153.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            2012..2782
                     /locus_tag="OO349_003173"
     CDS             2012..2782
                     /locus_tag="OO349_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR5868154.1"
                     /translation="MTTQSHHDHVEKQFSSQACEYLTSTVHASGRDLQRLAVRLADYP
                     GASALDMGCGAGHASFVAAQNVSAVVAYDLSAQMLDVVAQAAEARQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRMIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFCSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(2830..4188)
                     /gene="mltD"
                     /locus_tag="OO349_003174"
     CDS             complement(2830..4188)
                     /gene="mltD"
                     /locus_tag="OO349_003174"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HCR5868155.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDDDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAN
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(4260..5015)
                     /gene="gloB"
                     /locus_tag="OO349_003175"
     CDS             complement(4260..5015)
                     /gene="gloB"
                     /locus_tag="OO349_003175"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HCR5868156.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAITANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVITTPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTALQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSIFPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINGINEETLLQQPEERFAWLRSKKDRF"
     gene            5049..5771
                     /locus_tag="OO349_003176"
     CDS             5049..5771
                     /locus_tag="OO349_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR5868157.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDICLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            complement(5768..6235)
                     /gene="rnhA"
                     /locus_tag="OO349_003177"
     CDS             complement(5768..6235)
                     /gene="rnhA"
                     /locus_tag="OO349_003177"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HCR5868158.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            6300..7031
                     /gene="dnaQ"
                     /locus_tag="OO349_003178"
     CDS             6300..7031
                     /gene="dnaQ"
                     /locus_tag="OO349_003178"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HCR5868159.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAVF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            7164..7240
                     /locus_tag="OO349_003179"
     tRNA            7164..7240
                     /locus_tag="OO349_003179"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7198..7200,aa:Asp,seq:gtc)
     gene            complement(7371..8417)
                     /locus_tag="OO349_003180"
     CDS             complement(7371..8417)
                     /locus_tag="OO349_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImpA family type VI secretion system protein"
                     /protein_id="HCR5868160.1"
                     /translation="MEIFLSDPISAECPCGPDLEYDPEYLLLFTRAAPREEAQYGDFV
                     STPENINWAELERDAHRLLMRSKDIRILVVLLRCRIQQAGARGLSEALTLLETLCSTY
                     PDAIHPQLLATEDITAEDAAVARSNALAALLDHEGVMADIRGITLSNNAAMRLQVRDV
                     ERSLSALRPADALAPESVRQQLADLEARGTLPLDAFRQAAETTERLQRHARETLNDQA
                     PDFSRLTQLLALLPGAVQSTTPEILPQPQAEQPENATIVHTEQMQAEQIALPVHIAEE
                     ISPMTGAEPRQIRDRNDALERLRVIRRWFEHSEPSSPTIPLLRQAERLVGKRVSEVIN
                     EIPVELLEKWDALE"
     gene            complement(8428..9413)
                     /gene="tssG"
                     /locus_tag="OO349_003181"
                     /pseudo
     CDS             complement(8428..9413)
                     /gene="tssG"
                     /locus_tag="OO349_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601590.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
     gene            complement(9410..11293)
                     /gene="tssF"
                     /locus_tag="OO349_003182"
     CDS             complement(9410..11293)
                     /gene="tssF"
                     /locus_tag="OO349_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HCR5868161.1"
                     /translation="MDPRLLEYYNRELSYLRETGAEFAARHPKVAARLGMQGTDIADP
                     YVERMVEAFSFLTARTQLKIDAEFPRFTQRLLEVVSPNYVTPTPSMSVAQLHPDTEEG
                     DLAKGFTVPRDTAFFSAIPEGESTACQFRSSQDVTLWPLAIEEARLTAAPPDMPALHR
                     YLPANIHVAGALRITLRTFGELTFSQLAGLDRLPFYLCGEERTASHLLELLHTSAIAT
                     LAGIPGHFDGALDVNLQQPVMYEGLEPDQGLLPLAWNVFHGHNLLHEYFACPERFYFF
                     TPTGLSAGLQKIDGGVAEIVILLNRLPPDWLIHQTNAAQFSLFCTPVINLFPRVTARI
                     DVTHSTTEQHLVVDRTHPLDYEVFSVQEVEGLETDTTRKMAFRPLYHTRNNDEGNHGR
                     YFSLRREPRRLSENARRYGTRTPYTGSEVFLSLVDQYEAPYPENLRHITITAMVTNRD
                     LPCLIARNGRDDLTVDAAIPVAGVGLIRPPRSPQPPLAEREMAWRLIRQLSFNYLPLA
                     DLDHRTGGQALRDLLNLFIPAHDSPQSRQVRSLIGCKTTPVTRRLPGSGLLVYGRGVS
                     CELTVDEEGFSGISPYLFGLVLEHYIARHVSINTFSQMTLHSMQRGKIMTWPVRAGQR
                     GSV"
     gene            complement(11309..11803)
                     /gene="tssE"
                     /locus_tag="OO349_003183"
     CDS             complement(11309..11803)
                     /gene="tssE"
                     /locus_tag="OO349_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HCR5868162.1"
                     /translation="MSASRQRPGNHLLPTLFDRLCDDAPNQKRDHGISVSPVQIKEII
                     RRDLSFLLNTVSHEDDIDAARYPYAAASVLNYGLPPLAGSFLYEHKWDDIRRAILRTI
                     TRFEPRLKASTLQIIPLQDERRQSGHNTLQFEIRGEILTQPYPTAFRVRSALDMEQSR
                     ITFF"
     gene            complement(11800..12630)
                     /locus_tag="OO349_003184"
                     /pseudo
     CDS             complement(11800..12630)
                     /locus_tag="OO349_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233830.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="impE family protein"
     gene            complement(12617..13531)
                     /gene="tagK"
                     /locus_tag="OO349_003185"
                     /pseudo
     CDS             complement(12617..13531)
                     /gene="tagK"
                     /locus_tag="OO349_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804008.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagK"
BASE COUNT         3101 a         3524 c         3612 g         3298 t
ORIGIN      
        1 ccgggagctg tttttacatg ctcgctaagg aaatcgataa aagcccggat gcgcgtactt
       61 accgcacggt cgctgtaata gacggcactg aatggcattt ccactggcaa cactttatct
      121 gccattaact ccaccaattc tccgcgagcg atttctttgt cgatcatgta gtcggacaaa
      181 cacgcaatcc cgttgccact caggcaaagc tgtttcagtg tttccccact attggatgac
      241 aaaccgtact tcacctcatg taattgtcca tcactacagg ctatcggcca ggtattgagg
      301 gaagcgggtt cagtgaatcc caggcaaaca tgttgcttta aatcgtcgat cgtttctggc
      361 ttcccgtagc gggaaatata atcgggggag gcgataattt ttcgataact gttaaataac
      421 ggcctggcac gtaagctgga gtccgttaac gtaccagcgc gtatcgcgac atccactttt
      481 ctttcgatca aattaataat cgtttcggag gagactagcg ataaagtgac ttccggatag
      541 cgttcacgga aaggcttaat taacggcatc agaaagtgca gcaccactgg agttgcggca
      601 tcgatccgta acagtccacg cggtgtatta cgcgtctcca taatttctga ttctgccgct
      661 gccatctcct gcaaaattga ctgtacgcga cgaaaataac gctcgccttc ttccgtcagg
      721 ctaagttgcc gcgtggtccg attaagcagg ctaacgccaa gtttcatttc cagctttttc
      781 accgcccggc ttaccgctga gtttgcttgc cctaattgtt ccgctgcccg gctaaagctg
      841 ccgctttcga cgaccgaaac aaaaatggcg agttcttccg acgtggcttt catttttgct
      901 cctgttgcaa aatagatgag atattttgaa tttatttgtc attaaaccat caggatgcgt
      961 gatatgtcat ccgatttaat gttctcaata atgagcaaat ttctgacagg tgtaagcact
     1021 tgcttacata acaatataca attgctcgtt gaaagagtga gctaaaatcc ctataacagt
     1081 agaaccctcc cgagtgcgga agggttgacg taatagaggt ttcaaagtca aaagtgcgaa
     1141 aaaacaccta tgccatgcgc tatgttgccg gacaacctgc ggaaaggatc ttaccgccgg
     1201 ggtcttttgc gagcatcggc caggcattac cacctggaga accgttaagt accgaagagc
     1261 gtattcggat cctggtgtgg aacatataca aacagcaacg cgctgaatgg ttgtcggtat
     1321 taaagaacta cggcaaagat gcacatctgg tgttattgca ggaagcgcag acaacgccag
     1381 agttagtaca gtttgcgacc gctaactatc ttgccgccga tcaggtgccc gctttcgtgc
     1441 tgccacaaca tccttccggt gtaatgaccc tttcggcggc gcatccagtg tattgctgcc
     1501 cgttacgtga acgagaaccc attttgcgtc tggcgaagtc ggcactggtg acggtctatc
     1561 cattacctga cacccgcctg ttgatggtgg ttaatataca cgccgtcaac ttcagtctgg
     1621 gcgtggatgt ctatagtaag cagttacttc ctattggtga tcagatagct caccacagcg
     1681 gcccggtcat tatggcggga gatttcaatg cctggagccg tagaaggatg aacgcgttat
     1741 atcgctttgc gcgggaaatg tcgctgcgcc aggtgcgttt taccgatgat cagcgccgtc
     1801 gggcgtttgg ccgcccgctc gattttgttt tctaccgtgg tctgaacgtc agcgaagctt
     1861 ctgtattggt tacgcgcgct tccgatcaca atccgctact cgttgaattc agtcccggca
     1921 agcctgataa ataaggtatg tcaggtctgc cacagggcag accaacgttt ggcgctgtcc
     1981 ttttatactc aaacaacaaa aggacagcgc aatgacaaca caatcccacc atgaccacgt
     2041 agaaaagcag tttagctctc aagcctgcga atatctgacc agtaccgtac atgcatccgg
     2101 acgagattta cagcgcctgg cggtgcgtct ggctgattat cctggtgcaa gtgcacttga
     2161 tatgggctgc ggagcagggc atgccagctt tgttgctgcg caaaacgtga gcgcggtggt
     2221 ggcgtatgac ttatctgccc aaatgctgga tgtcgtggca caagctgccg aagcccggca
     2281 actgaaaaat atcaccaccc gccagggata tgccgaaagt ctgccatttg ccgataacgc
     2341 atttgatatt gttatcagcc gttattctgc ccatcactgg catgatgttg gtgcagcact
     2401 gcgtgaagtg aaccggatat tgaaacctgg cggtaggatg attgtgatgg acgtaatgtc
     2461 tccgggtcat ccagtgcgcg acatctggtt acagacggta gaagcattac gcgatacctc
     2521 tcacgtacga aactacgcca gcggtgagtg gttgaggtta atcaatgaag ccaatctgat
     2581 agttgataat ttaattacag ataagttacc gctggaattt tgttcatggg tcgcgagaat
     2641 gcgtacgcca gaagcgttag tagacgctat tcgcatttac caacagagcg catcgacaga
     2701 ggtgagaacg tattttgcct tgcagaatga cggctctttc accagtgata tcatcatggt
     2761 agaagcacat aaagcggcat aaataaaaaa ggcaccgggg gaatcggtac ctttttatta
     2821 tctggtttgt caggaatctg gcatgttgtt gtttttcaca aacaacgtca gcttgtcgcc
     2881 tggttgcaga ttcgcagtgt cgctgttcca gcgcatcaca tctttgatgt tcacgccgtg
     2941 gcgtttagca atgcttgaaa gcgaatcgcc tttgcgcaca cgatacgtaa tgctatcgct
     3001 gttgtttgcc agtcgctgtg cgctactgcc tgcaccaatc gtcaaacttt ggcctggctt
     3061 cagcttagat ccgcgcagtt tgttccactg ctgcaaatct ttggtgctta cgccgagacg
     3121 tgaagcgatg cttgaaagcg tgtcgccaga gcgtacggtg taaacacggc tgttaagcgg
     3181 cgtattgttg gcaaccagcg tcgactgtac agcagcaatt tcgcctgaag cgagagattc
     3241 acgcagttga tctgcatgct tctttggcac catcacgtac tgcggcccac ttgcgcccag
     3301 cgtggagcct ttcacgccag cgttgaatgt cttcagcttg ctgacggaaa tccccgccat
     3361 atctgcaacc ttcgccattt caaccgggct actcaggtgc acacgcgcca gagcacggct
     3421 ttcatcggtc gttggcagac gtacgccata acgcttgctg tttttgagaa tatcactcaa
     3481 tgccagcatt ttaggcacgt actgcttcgt ttcctgcggc aacggtaacg accagaagtc
     3541 cgtggatttc ccacgcgctt tgttcgtttt aattgccttc atgacccgac cttcgccgct
     3601 gttataagcc gctacggtca gaagccagtc gtcgtcaaac attttgttca gacgctgcat
     3661 catattcagc gcggcagttg ttgaagcaac aacatcgcga cgcgcgtcat aattgcgggt
     3721 ctgtttcaaa ccataattgc gccccgtgct cggaatgatc tgccagatgc ctgcggcatt
     3781 ggcgccagac gttgcgtgag gatcaaaagc gctctccact atgggtagta gtaccagttc
     3841 cataggcatg ttacgttttt taacttgccc tgctatccag tacatatacg gctctgcccg
     3901 taaagttaca tcgtggagat agctcttatt gcgtaaatat ttctgtttct gttcgcgaat
     3961 ccggtcattt tccggaattc ccatctttag ctcgtcgcca atgaaagccc acaagtcacc
     4021 atctggcgcg atagacgtcc catcgtccat ccatcgtgcc tgacttgtaa actttgctgc
     4081 ttccccttga ccagctgcag aaaggctctg tgcgtgctgt tgaacgttgc cggtactctg
     4141 gcaacccacg agcaggacag aggcgagtaa tatcgctttt gccttcatgt gtgtgtcaat
     4201 agttgcttaa aagacgaccg atcataacgg cgaacggagc cgatgacaag aaagttttat
     4261 cagaacctat ctttctttga ccttaaccat gcaaaacgct cttcaggttg ttgcaataat
     4321 gtttcttcat taattccatt aattaaatca atatcttccg ttcttaaaaa aacattaatt
     4381 tgccgctcat ttttcagaat tacgggtagt gttatttgat tttttgcccg taactcctta
     4441 actttacgat aataatcatt tatggacaaa tcgtgcggaa aaatactcaa agcaaacttc
     4501 atatttgata acgtatattc atgagcacaa cataccaatg tatcgtcagg taacgcactt
     4561 agctttttaa gtgattgata catttgtaaa gctgtccctt caaacaaccg accacaccca
     4621 ccagaaaaca gcgtgtcgcc gcaaaataga taaggtttac tgaagtaaca gatatgtcct
     4681 aaagtgtgac ccggtgtggt aattacacta aattcatgcc ctaaaacgaa ggcagtttcg
     4741 ccatctttga ctacctgtgt tgttccctta tcttgtgtct cttgtggacc atacaccaca
     4801 atttgcggaa acttttccac cagttctttt acgccgccaa cgtgatcgtg gtgatggtgg
     4861 gttagaaata tggcctccgg ttgccagtta ttggcggtaa tggcgtttaa tactggctct
     4921 gcgtctccgg gatcgacaat caggcagcga cctgcctcat cattcaaaac ccagatgtaa
     4981 ttgtcatcaa aggcgggaat actgttaaga ttcatagatt acctctcagt gtaaaacgga
     5041 aggttgtgat gaaaccggca agagtccctc aaactgtcgt ggctcctgat tgctggggcg
     5101 atttgccctg gggagagctt tatcgcaagg cgctggagcg ccagctcaac ccgtggttca
     5161 ctaaaatgta tggttttcat ctgcttaaga ttggcaattt aagcgcagaa atcaattgcg
     5221 aagcgtgcgc ggtttctcat caagtgaatg tttctgcgca aggaatgccc gtccaggtgc
     5281 aggcggatcc acttcatctt ccttttgccg ataaatccgt tgatatttgt ctactggcac
     5341 ataccttgcc gtggtgcacc gatccgcatc gtttattacg tgaagccgat cgggtactga
     5401 ttgatgatgg ctggctggtg attagtggct tcaaccccat cagtttaatg ggattacgca
     5461 aacttgtgcc ggtattgcgc aaaacctcgc cctataatag ccggatgttt actctgatgc
     5521 ggcaactgga ctggctctct ttgttgaatt ttgaagtgct gcacgccagc cgtttccacg
     5581 ttctcccgtg gaacaaacac ggaggaaaac tattgaatgc gcatattcct gcgcttggtt
     5641 gcttacaact tattgttgcc cggaaacgga ctattccttt aacgctaaat ccgatgaaac
     5701 agagtaaaaa caagccacga attcgccagg cggttggagc cacccggcaa tgtcgtaaac
     5761 cacaggctta aacttcgact tggtagcccg tatcttccag tgtgggattc atcgccgcgg
     5821 cacgagccag ttcatcacag cgttcgtttt ccgggtgtcc ggcatggcct ttaacccatt
     5881 cccatttgat ttgatgctgc cccaatgcag catcaagacg ttgccagaga tcgacatttt
     5941 ttactggttt tttgtctgct gttttccagc cacgtttttt ccagttatgg atccactggg
     6001 tgataccctg gcggacatac tggctgtcgg tactcaaaat gacttcgcaa tgttctttta
     6061 acgcctccag cgcgacaata gcggccatca actccatacg gttgttggtg gtgcgggtgt
     6121 agcctgcgct aaaggttttc tcgcgtccgc gatagcgtaa aatagcgccg taacccccag
     6181 gtccaggatt acccagacac gaaccatcgg tgaaaatttc tacctgttta agcatctctg
     6241 gtagacttcc tgtaattgaa tcgaactgta aaacgacaag tctgacataa atgaccgcta
     6301 tgagcactgc aattacacgc cagatcgttc tcgataccga aaccaccggt atgaaccaga
     6361 ttggtgcaca ctatgaaggc cacaagatca ttgagattgg tgctgttgaa gtagtgaacc
     6421 gtcgcctgac gggcaataac ttccatgttt atctcaaacc cgatcggctg gtggatccgg
     6481 aagcctttgg cgtacatggt attgctgatg aatttttgct cgataagccc acgtttgccg
     6541 aagtagccga tgagtttatg gactatatcc gcggcgcgga gttggtgatc cataacgcag
     6601 tgttcgatat aggctttatg gactacgagt tttcgttgct taagcgcgat attccgaaga
     6661 ccaatacctt ctgtaaggtc accgatagcc ttgcggtggc gaggaaaatg tttcccggta
     6721 aacgcaacag cctcgatgcg ctatgtgccc gctacgaaat agataacagt aaacgaacgc
     6781 tgcacggggc attactcgat gcccagatcc ttgcggaagt ttatctggcg atgaccggtg
     6841 gtcaaacgtc gatggctttt gcgatggaag gagagacaca gcagcaacaa ggtgaagcaa
     6901 caattcagcg cattgtacgt caggcaagta agttacgcgt tgtttttgcg acagatgaag
     6961 agctggcagc acatgaagcc cgtctcgatc tggtgcagaa gaaaggcgga agctgcctct
     7021 ggcgggcata aatacatgta aaaggcgcta aaaatagcga cttgggcgat ttttgcagca
     7081 aacgattcaa aagatgagaa aaatcgttga cgaaggtcga ggcaatccgt aatattcgcc
     7141 tcgttcccaa cggaacacaa cgcggagcgg tagttcagtc ggttagaata cctgcctgtc
     7201 acgcaggggg tcgcgggttc gagtcccgtc cgttccgcca atattcagaa agcccgaatc
     7261 agtaatgatt cgggctttcg tcgttttggt gaagtgagaa tttttcactc cgaagtaaaa
     7321 cggatatgat ttaaaaaatg gccagacatt ataaattctg gccacttctg ttactccagc
     7381 gcatcccatt tctccagcag ttctactgga atttcattta ttacctccga gacgcgtttt
     7441 ccgaccaggc gttctgcctg gcgcagcaaa ggaatggtcg ggctgctcgg ttcgctgtgc
     7501 tcaaaccagc gacggatgac acgcaggcgt tccagcgcgt cgtttcggtc gcggatctgc
     7561 cggggttctg cgccggtcat cggtgatatt tcctccgcga tatgcaccgg tagcgcaatc
     7621 tgctctgcct gcatctgttc agtatgaacg atggtggcgt tttccggctg ctcagcctgt
     7681 ggttgtggca ggatctccgg cgtggtgctc tgcactgctc ccggtaacag ggccagcagt
     7741 tgcgtcagac gggagaagtc cggtgcctgg tcattcagcg tttcacgcgc atggcgttgc
     7801 agccgctcag tggtctctgc tgcctggcga aaggcatcca gcggcagtgt gccgcgcgct
     7861 tccaggtctg ccagttgctg gcgtaccgat tctggtgcca gcgcatccgc cgggcgtaat
     7921 gcagatagcg aacgctccac atcccgcacc tgaaggcgca tggcagcatt atttgacagt
     7981 gtaatgccac gaatatccgc cattacacct tcgtggtcca gcaacgccgc cagcgcgtta
     8041 ctgcgtgcca ccgcagcatc ttcggcggtg atatcttcag tcgcgagcag ttgaggatgg
     8101 atggcgtccg ggtacgtgga gcacaacgtt tccagcagcg tgagcgcctc cgacagtccc
     8161 ctggcaccgg cctgctggat acggcagcgc agcaggacaa ccagaatgcg gatatctttg
     8221 ctgcgcatca gcagacgatg ggcgtcccgc tcaagctccg cccagttaat gttttccggc
     8281 gtactgacaa aatcgccata ttgcgcttct tcacgcggtg cggcgcgggt gaacagcagc
     8341 aggtattccg gatcgtactc cagatccggg ccgcaggggc attccgccga aattgggtca
     8401 gacaggaata tttccattta attatcctca gtgcgccagg tactgttctg gttcgaaaat
     8461 catgcccgtc accgggttgt cgtgcatggc tttacccgcc catgcagtcc aacccagttg
     8521 gtacggacca ccggtaacag ccggaggggc ggcgtggggc tttagctgta gctcaatttc
     8581 ccagcagtat tcataaccac taaaggcgcg gaccagttct gtcaggacgg gcaggttatt
     8641 gctgccaggc agaaaacgtc ggtaatcctc cagcgtcagc gggccgataa ccagccggaa
     8701 cttatactgc atgtccggca cggcctggcc gatgagtgca ccattgccaa taacggaaga
     8761 gggggcaggc atccccagcc gggtgatctc atcgtccgcc acggctatcc agtgcaggac
     8821 atactcctgt atccggaaag gtacaccgaa atagtgcgcc agcgtggtgg ccagaccgtc
     8881 cggattgcgg gactcacgca ccagatgggc cgatgccgcc agacgagcgt gatctggcaa
     8941 cgggctgtca gcgctctccc gcaaatcctg cccgctgagg ctggcgacat aaaaggcaaa
     9001 gcggtcatgt tgcggtttat ccagaccgcc cccagcggac tgggcgctgc gccatgcctg
     9061 gtaaaactgc gtcagccagc ggtgatgaaa aagatcagaa aaacgggtca gcgtcggctt
     9121 tcagtccggt tacgcgccag ttcggtgtag tgcagcggca gtggaccgtt cggcccccac
     9181 agcccgaggc tgtacagact gatgtgcagg cgcccttcct gccagcccac actggcgatt
     9241 tctcgtggcg caaaggtcag cgcgggcgtt tgtccgaggc gaaatttttc catccgtggt
     9301 tgccaggtgt tgcccgccgg aacggtacac aactgcacat ccacgcggcg catcaggttc
     9361 agaaaaccgt aacgccaggg gggggcctgc gcctgagtga aatcgttgct catacgcttc
     9421 cccgctgtcc cgccctgaca ggccaggtca taattttgcc gcgctgcatg ctgtgcagcg
     9481 tcatctgcga gaaggtatta atggaaacat ggcgggcgat atagtgttcc agcaccaggc
     9541 cgaacagata cggactgatg ccggaaaaac cttcttcatc caccgtcagt tcacaactga
     9601 cgccacgccc atagaccagt agtccggaac cgggcagacg gcgggtgacc ggggtcgttt
     9661 tacaaccaat caggctgcgc acctggcgcg attgcggact gtcatgagca ggaataaaca
     9721 ggttcagcag atctcgcaac gcctggccgc cggtacggtg atccagatcg gccagcggca
     9781 gataattaaa agataactgg cggatcaacc gccatgccat ctcacgttca gccagcgggg
     9841 gctgcggcga gcgtggcggg cggataagcc ctacgcccgc caccgggatg gctgcatcca
     9901 cggttagatc gtcccggcca ttgcgggcaa taagacaggg cagatcgcgg ttagtgacca
     9961 tggccgtgat ggtgatgtgg cgcagatttt ccgggtacgg cgcttcatac tggtcaacca
    10021 gcgacaggaa cacttccgag cccgtatagg gggtacgggt gccgtaacgg cgggcgtttt
    10081 ctgataagcg gcggggctcc cggcgcaggg aaaaataacg tccgtgattg ccttcatcat
    10141 tattgcgggt gtggtagagc gggcgaaagg ccatcttccg tgtggtgtcg gtttccagcc
    10201 cttccacttc ctggaccgag aacacctcat aatccagcgg atgggttctg tccaccacca
    10261 gatgctgttc cgtcgtgctg tgcgtgacat cgatccgggc ggtgacgcgg ggaaacaggt
    10321 tgataaccgg ggtacagaac aggctgaact gtgccgcatt tgtctggtgg atcagccagt
    10381 ccggcggcag gcggttcagc aggataacaa tctccgccac gccaccgtca attttctgta
    10441 atccggcaga caggcctgtc ggggtgaaaa aatagaagcg ttccggacag gcaaaatatt
    10501 catgcagcag gttatgaccg tgaaacacgt tccacgccag cggcagcagc ccctgatccg
    10561 gctccaggcc ttcgtacata acgggctgtt gcagattcac atccagcgcg ccgtcaaaat
    10621 gccccggaat gccagccagg gtcgcgatgg cgctggtatg tagaagttct aacaggtgtg
    10681 aggccgtacg ctcctcgccg cagagataaa acggcagcct gtccagtccg gcaagctgac
    10741 tgaacgttag ttcaccaaag gtgcgcaggg taatacgcag cgccccggcg acgtgaatgt
    10801 tggccggcag gtagcggtgc agggcaggca tatccggtgg cgcggcggtc agtcgcgcct
    10861 cttcgatggc gagcggccac agcgttacat cctggctgct gcgaaactgg caggcagtgc
    10921 tttcgccttc cggaatggcg gagaaaaagg ctgtgtcgcg cgggacggta aatcctttcg
    10981 ccagatcgcc ttcttccgta tcgggatgca actgcgccac gctcattgag ggcgtgggtg
    11041 tgacgtaatt ggggctgacc acttccagca ggcgctgggt gaaacggggg aattcagcgt
    11101 caatttttag ctgcgtgcgg gcggtcagga agctgaaggc ctcgaccatt cgctctacat
    11161 acggatcggc aatatccgtt ccctgcatgc ccagccgggc ggcgactttg ggatgacggg
    11221 cggcaaattc ggctcccgtt tcccgcagat agctcagttc gcggttgtag tattccagta
    11281 gccgtggatc catcggtctc tctctgaatt aaaaaaacgt aatgcgactc tgctccatat
    11341 ccagcgcgct acgtacccgg aatgcagtcg gatacggctg cgtgaggatc tcgccgcgaa
    11401 tttcaaactg taatgtgtta tggccactct ggcggcgctc atcctgaagt gggatgattt
    11461 gcagagtgga cgctttcagc cggggttcaa accgggtgat ggttcgcaaa atggccctgc
    11521 ggatatcgtc ccacttgtgc tcatacagaa agctgcccgc cagtggcggc aggccgtagt
    11581 taagtacgga cgctgccgcg tacggataac gtgcagcatc aatatcatct tcatggctga
    11641 cggtgttgag cagaaatgac aaatcccggc ggatgatctc cttaatctga accggactga
    11701 cgctgatacc gtggtcgcgt ttctggtttg gggcgtcgtc acacaggcgg tcaaacagcg
    11761 tgggcagcag gtgattgcct ggacgctggc gggaagcgct cacgcgtcgc ttccttcagc
    11821 ggtgttaaag gtacaggtgg acaaatccag caggctgata tcgccgtgac cggtgagcca
    11881 caccctctga cccagcgcct gtaccagtgt ctctcccgga ccgtcctgcc agaccgtttc
    11941 ccggcacaga cgcagggagt cggcagcctt ttcagagccg ctgtagcggg gaaacagcta
    12001 ggcactgtgt gtatcgccat tgttcagggt gacgttgacg ggtttccaga tcaaatcgat
    12061 cagatgcgca ggccgcggcg atttcagcga acagatttgt gaaaacggca gccagatata
    12121 tgctccgccc gcgatcagtt cgagtaccgg accggtacgg gagtcgctgt cgctaatcca
    12181 gtcaaatgcg ccgccattcc actgcccgga agtgtctgtt atggcctcaa gcgcgcggct
    12241 gcggtgagca tcagcttcct gcgcttcacc acgggcgtta caggcgaggg cggtcagcag
    12301 atcctccatc caggcgggcg gcgggagaat aacaccgggc cgctgttcgc cctgaaagca
    12361 ggcgtggcgg tatatctcac agcggatcag ctcgccaaat acctgcgctt cgcgagtata
    12421 atttgcgtcc atccgggcgc atagctggat ctgttgcagt gcccgcgacc agtcgcctgc
    12481 cacgcacaat aactgaaaca ggctgtggcg gcgggaggcc tgcgctgggt gcatcctgac
    12541 ctgttgctct gccatctgaa tgccctccac cacggaataa tcccgcagca gcgccgacaa
    12601 ggtaatcggt agctcgttat tgtttgtcat gatatatctc tccatgttgt gccgggttta
    12661 tccggtaatg gctgtcaatg ccaatgatgc gatgctcttt ccgtgtcaga tctggcagta
    12721 cggtgttatg cgctgtttcc ggggcgtatt ccggggaaag taaatgcagg atatcagggg
    12781 gagtcttttc gacaggccag atagtttcat cttcgccagt ggcatccagt acattgagaa
    12841 tagcgtcaat gcttaaccca ccagtgacca tatcctgcaa tgtctcggct tcggtgacca
    12901 ggtcacgcaa tgaatccaga tcctgcgagg agggctcttc gttaaaagga aaaggatcag
    12961 catggcgatt aagatcttgt tcgggagata acaacgcctc gcggtattcc tgataaagtc
    13021 gggtcagggc atttcctttt tccgcacctt ctccggtgga ggaggctcca tgaacaggaa
    13081 tgatatcgaa tggattttgt gatgtcagat gctgctgcct gaaccaatcc agatccagat
    13141 attcggttat tgcttccgac gtgatggcag tctgcgatat cgtttttact gtgctcaggt
    13201 tcggagatga tggattgcca tgtacaaggc actatgaaga taacccccat tctatcaggt
    13261 cgccttcatt cagacgcata cggtgatgag gttccacaat ctggtcgttt accgtacagc
    13321 aatattcggt agactgattg ataatccacc atgcttcttc gtggcgggtc agagtgaggg
    13381 caatctttac atcatcattc accttaaaaa caggaatcca gggagaggta acggtgaaga
    13441 taatttgtgt tccttcagtc gcgtaaattg tgttatcggc gatcccgtgc gcctgtattt
    13501 tttggagtcc ccacgtaata tttggtttca ttgtg
//
LOCUS       DAJTQY010000100        13528 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.100, whole genome shotgun sequence.
ACCESSION   DAJTQY010000100 DAJTQY010000000
VERSION     DAJTQY010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13528
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.100"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(<1..830)
                     /locus_tag="OO349_003186"
     CDS             complement(<1..830)
                     /locus_tag="OO349_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase family protein"
                     /protein_id="HCR5868163.1"
                     /translation="MAISDTKLRTIYGKPYSGPQEVADADGLSVRISPKGVIQFQYRY
                     RWHGKPNRLGLGRYPSLSLKDARQITADLRKLYFSGTDPRTYFEEKVENSMTVAQCLD
                     YWFDNYVSTTLREKTQALYRLTVMKRMHDAFPNRPASSITVKQWVDLLTEEEKDNPRR
                     ARQVLSQLRSAISWCMRRQLIDSCAIMSIQPRDFGSRAEVGDRVLSYHELAKIWLAIE
                     RSRASTSNKLLHQMLMLWGARLSELRLATKTEFDLLDNVWTVPKEHSKMGNVIRRPIF
                     "
     gene            complement(1036..1755)
                     /locus_tag="OO349_003187"
                     /pseudo
     CDS             complement(1036..1755)
                     /locus_tag="OO349_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
     gene            complement(1771..2103)
                     /gene="cusF"
                     /locus_tag="OO349_003188"
     CDS             complement(1771..2103)
                     /gene="cusF"
                     /locus_tag="OO349_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HCR5868164.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVV
                     KGVDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(2127..3509)
                     /gene="cusC"
                     /locus_tag="OO349_003189"
                     /pseudo
     CDS             complement(2127..3509)
                     /gene="cusC"
                     /locus_tag="OO349_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706419.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
     gene            3666..4349
                     /gene="cusR"
                     /locus_tag="OO349_003190"
     CDS             3666..4349
                     /gene="cusR"
                     /locus_tag="OO349_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HCR5868165.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTLSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            4339..5781
                     /gene="cusS"
                     /locus_tag="OO349_003191"
     CDS             4339..5781
                     /gene="cusS"
                     /locus_tag="OO349_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HCR5868166.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIIIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFARDAIPDKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKRQVAGDPLMLRRALSNLLSNALRYTP
                     TRETIVVRCQTVDHQVQVSVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVITLPA"
     gene            5931..8168
                     /gene="nrfB"
                     /locus_tag="OO349_003192"
                     /pseudo
     CDS             5931..8168
                     /gene="nrfB"
                     /locus_tag="OO349_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706416.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage adsorption protein NrfB"
     gene            8155..11123
                     /gene="nfrA"
                     /locus_tag="OO349_003193"
                     /pseudo
     CDS             8155..11123
                     /gene="nfrA"
                     /locus_tag="OO349_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
     gene            11124..12014
                     /locus_tag="OO349_003194"
     CDS             11124..12014
                     /locus_tag="OO349_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HCR5868167.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRFQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQTGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDMLYFSLRYLPVAHGILEY"
     gene            12197..12958
                     /gene="envY"
                     /locus_tag="OO349_003195"
     CDS             12197..12958
                     /gene="envY"
                     /locus_tag="OO349_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HCR5868168.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKKLTCVKPWSRLATPVIACHSRTPEVFRLAT
                     NHSKQQSSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYSLTPLNYLAKQREKVMW"
     gene            complement(13004..13080)
                     /locus_tag="OO349_003196"
     tRNA            complement(13004..13080)
                     /locus_tag="OO349_003196"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13044..13046),aa:Arg,seq:tct)
     gene            13323..>13528
                     /locus_tag="OO349_003197"
     CDS             13323..>13528
                     /locus_tag="OO349_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HCR5868169.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFL"
BASE COUNT         3303 a         3506 c         3427 g         3292 t
ORIGIN      
        1 tcgaagattg gacggcggat aacattaccc atcttgctat gctctttcgg tacggtccat
       61 acgttgtcca gcaggtcaaa ttctgtcttt gttgccagcc taagctctga gagcctcgcc
      121 ccccacagca taagcatctg atgaaggagc ttatttgacg tagacgcacg gcttctttca
      181 atagcaagcc aaatcttagc cagttcgtga tacgacagta cccgatcccc tacctcagcg
      241 cgggagccga agtcccttgg ctggatgctc ataattgcgc aactatctat caactgacgc
      301 cgcatgcacc aactaattgc tgatcttagt tgacttagca cctgccttgc tcggcgtgga
      361 ttatcttttt cttcttcggt aagcaggtca acccattgct taaccgtgat agaagatgcc
      421 ggacgattag ggaaggcgtc atgcatgcgc ttcataaccg ttaatcgata aagtgcctgg
      481 gtcttttctc tgagagttgt agagacgtag ttgtcgaacc agtagtcgag acactgggcg
      541 accgtcatgg agttctccac cttctcttca aaataggtgc gtggatccgt tcctgagaaa
      601 tagagctttc gcaagtcagc agtgatctgt ctggcatcct tcaaagacag ggatgggtat
      661 cgaccaagcc caagtcgatt aggcttgcca tgccagcgat agcggtactg gaactggatg
      721 acccccttcg gtgaaattcg tacgctgagg ccatcagcat cagccacttc ttgtgggcca
      781 gaatatggtt taccataaat agtacgcagt tttgtgtcgc ttatagccat aaataatatt
      841 tggtacgctc agaaataatg gtttggtaca catctggtac acaatatcac atggacaaca
      901 gcgcacagta aacaactata tttgacgttc gtagacatac tcaggtgaaa aaagggttgt
      961 ttttcgagtt ataacagaca gttaattaac tacagtggac aatgctagac aatgacagac
     1021 acagataagc aacctacctt cctctttcgc gaccacgtta tctttggcga tattcattcc
     1081 cgcgcgcaga tcaaacgcgg tgatcacgcc atcaatgggc gctttgagcg taaagcgagt
     1141 ctggattttt tgcgtggcga tcaggtggcg aatatccgcc tccggcattc ccgccagtcg
     1201 cagccgctca agaatgcctt cagtctgggt cgccgtaccg ccggtttcgc gcaacagtaa
     1261 atactcactc tgcgcttcca cccagtcggg aatggtcagg tcgagaagcg gtgtgccctt
     1321 ttgtacttta tcgcccacgg taagcggata caccttgtcg ataaacccgg cagcgcgagc
     1381 ctgcacaatg gcatactgat actcgttgta actgacattc gccgggaaac tctgggcaaa
     1441 agtcagcggc ccgcgcgtga cggtagccgt tttcaccccc agattctgcg tctgagtcgg
     1501 gtcaatgcgc acaccagacg cagaactctc ttcatcggca tatttcggca ccagatccat
     1561 atccataaac ggcgatttac ctggtttatc gaaccgcgta ttgggataca tcgggtcgta
     1621 ccagaataag attttacgtt ctgcggtcgg cattttttct gtgtgcggtt ccgcctggtc
     1681 aaaccaggta aaacctgccg ctgaaataat accgcccgcg atcatgctgc cgataataag
     1741 cgcgattttt ttcatctcat taaacctggg ttactggctg actttaatat cctgtaataa
     1801 agaaaggttg ccctgctgga caaaattaaa cgccactttg tcgccggttt taatttcact
     1861 cattttcgtc tgcggggtga tggtaaagcg catggtcatc tccggccagt tgacggcagc
     1921 aatcggatcg tgatggatgg tgattttttt gctttccaga tcaacacctt ttaccacacc
     1981 ggtggcacta atgacctgtg gttgtgcttc gctcatggtt tcatgatgat gttcgttagc
     2041 ctgggcatta aagccaataa cggtaaacag actgaacatt gcgacttgca gtgctttttt
     2101 catttattct ctcctggagt taaaaattat tgctgccaac cgccccctaa ggcggtatac
     2161 aaataaattt cgttaacctg acgggcataa ttcaggtcaa gtaaagtttg tcgggttgca
     2221 aataaagaac gctcggcatc cagcacttcc agataactta ccgcgccgtg ctgatataat
     2281 gcccgcgccc gttgcagggt aatttgcagc gacgccagat aacgctgctg ggcgctgatt
     2341 tgatcgtcca ggctttgacg taatgccagc gcatctgcca cctctttaaa ggcgttctgg
     2401 attttctgtt cataattcac caccgactgc tgctggcgaa tttcggcgat atccagattg
     2461 gcctggttgc gtccggcatt aaaaatgggg atctcaattt taggaataaa attccacatc
     2521 ccgctgctag cgttaaataa tgacgacaga tcgctgctgg cggttgatat tccgctggtc
     2581 aggctgatag acgggaaaaa tgccgcacgc gcagcaccaa tattagcatt agccgccatt
     2641 aacgcgtgtt cagcttccat aatatccggg cgctgcaata agatttgcga cgacaagccc
     2701 gccggtaatt taacgctttg caggctgccg ctgtttactg tctgcgcttg cggtagcttg
     2761 ccgtagcttc ccaataacag ttgcaatgca ttattcgcct gcgccagttc cccctgacgt
     2821 ttagcgatgt cactgcgggt actttctatc accccgcgag cctgttccag cgccagaaca
     2881 ttgctgctac cggtcaacag ttgtttttcg acaaacgcat atgactgctg ataattacgc
     2941 agcgtttctt cggctatttg caattgcgca tacgccagct gctgattgaa atagctttgc
     3001 gcgacattag aaaccagcaa aatatgcacc gcgcgctggg cttcctcagt ggctaaataa
     3061 ttttggcgct cggcttcgct catgttcttt aagcgaccga aaaaatcgag atcaaagctg
     3121 gcgttaaggc cagtcgagaa ctcccgcgtc gtggctgaat cgcctttaag attgccgctc
     3181 cagctgccgc tgccctagcc attgagctgt gggtagcggt cggcatcggt cagacgatat
     3241 tgcgcccgtg cctcctgcac tttcagcgtc gccatgcgca aatcccggtt attcaccagc
     3301 gcctcgctaa tcagcgtctt cacctgatta tcaacaaaaa aggtgcgcca gcccacgttc
     3361 tggtagttat ccgctgcgtt aaccaggccg ttctggctga gtgagaactg ctgcggtacg
     3421 ggcattaccg gacgctgata atccggtgcc agtgaacaac cagttagcgc aagggccaca
     3481 caaaatggca gaagtttaca aggagacata ggctcataat ttctggtgat tttatgtcgc
     3541 caactttact cgccaggctc tgattttccg gtgacaggaa aatgacaaaa ttgtcatttt
     3601 gccaataagc gattgccatc tgatcccgct actctagaat tgcccgggca acatgcggag
     3661 gaaatatgaa actgttgatt gtcgaagatg aaaagaaaac cggagaatac ttgaccaaag
     3721 ggttaaccga agccggtttt gtggtcgatt tggccgacaa cgggctgaat ggctaccatc
     3781 tggcgatgac cggtgattat gacctgataa tcctcgatat tatgctgcca gacgtgaacg
     3841 gctgggatat cgtgcgcatg ttgcgctccg ccaataaagg gatgccgatt ctgttgctta
     3901 ccgcgcttgg caccattgaa catcgcgtca aggggctgga gttgggggca gatgactacc
     3961 tggtgaaacc gttcgctttt gctgaactgc tggcgcgggt gcgcactctc ctgcggcgcg
     4021 gggcggcggt gattatcgaa agtcagtttc aggttgccga tctgatggtc gatctcgtca
     4081 gccgcaaagt caccctcagc ggcacgcgca tcactctgac cagtaaagaa tttactctgc
     4141 tggagttttt tctccgccat cagggcgaag tgctgccccg ctcgcttatc gcctcgcagg
     4201 tatgggacat gaatttcgac agcgacacta acgccattga tgtggcggtg aagcggctac
     4261 gcggcaaaat cgacaacgac tttgagccga agctgattca aaccgtgcgc ggcgtgggtt
     4321 acatgcttga ggtgccggat ggtcagtaag ccatttcagc gcccgttttc gctggcaacc
     4381 cgcctgacct tttttatcag cctggccacc atcgcggcgt ttttcgcctt tgcatggatc
     4441 ataatccact cagtaaaagt gcattttgcc gagcaggaca ttaatgattt aaaagagatt
     4501 agcgccaccc ttgaacgggt actaaatcac cctgacgaaa cgcaagcccg acgcttaatg
     4561 acgctggaag atatcgtcag tggttattcc aacgtgttga tttccctggc agatagccac
     4621 ggcaaaacgg tgtatcactc ccccggtgcg cctgatatcc gcgagtttgc acgtgatgcc
     4681 atacccgata aagacgcgcg gggtggcgag gtgtatctcc tttccggccc gacgattatg
     4741 atgccaggcc acggtcacgg gcatatggaa cacagcaact ggcggatgat taacttgccg
     4801 gttggcccgt tggtggacgg caaaccgatt tatacgctct acatcgcgct gtcgatcgat
     4861 tttcatcttc attacataaa tgatttgatg aataaactta ttatgaccgc atcggtaatc
     4921 agcatcctga tcgtctttat cgtgctgttg gcggtacata aaggtcacgc gccgatccgc
     4981 agcgtcagcc gtcaaatcca gaatatcacc tcgaaagatc tcgacgttcg ccttgacccg
     5041 cagaccgtgc cgattgagct ggaacagctg gtactgtcgt tcaaccatat gatcgagcgt
     5101 attgaggatg tctttactcg ccagtccaat ttctcagcgg atatcgccca cgaaattcgc
     5161 acaccaatta ctaatctcat tacgcaaacg gaaatcgccc tcagccagtc gcgcagccag
     5221 aaggagctgg aagatgtgct ctactctaat ctcgaagagc tgacgcgaat ggcgaaaatg
     5281 gtcagcgata tgctgtttct cgctcaggcc gataacaacc agctaatccc cgaaaagaaa
     5341 atgctcaacc tggcggatga agtcggcaaa gtgttcgatt ttttcgaggc gctagcggaa
     5401 gatcgcggcg tggagttgcg gtttgttggc gacaaacgtc aggttgcggg cgatccgttg
     5461 atgctgcgtc gggcgctaag caacctgctt tctaatgccc ttcgttatac gccaaccaga
     5521 gagacaattg tagtacgctg tcagacggtc gatcaccagg tgcaagtttc cgtcgaaaac
     5581 cccggtacgc ccattgcgcc cgagcactta ccgcgattgt ttgaccgttt ctatcgcgtt
     5641 gacccctccc gccagcgaaa aggtgaaggt agcggtattg ggctggcgat agtgaaatcg
     5701 attgttgtcg cgcataaagg cacggttgcg gtaacgtcag atgcgcgggg gacaaggttt
     5761 gttatcacat tacccgctta attattcagc aaacggcaac ttttataacc agtgtaaaaa
     5821 taacgtgccg caatagtcat acatattaat ggtaaaaagc tgtcacaatt cataaaaaac
     5881 cttaatatac gccaccctaa acataaccag cgttaatgta aggtttttgt gtggactggc
     5941 ttcttgatgt ttttgctacc tggctctacg gcttaaaagt aatcgcgata acgttagcgg
     6001 tcatcatgtt catcagcggg ctggacgatt tttttattga tgtcgtctac tgggtacgcc
     6061 gcattaaacg caagttgagt gtttatcgcc gctacccgcg aatgagttac cgcgaactgt
     6121 ataaaccaga tgaaaaaccg ttagcgatta tggttccggc gtggaatgaa acgggcgtca
     6181 tcggcaatat ggccgagctg gcggcgacca cgctcgacta cgaaaactat catatctttg
     6241 ttggcaccta ccccaacgac cccgatactc aacgtgatgt tgacgaagtg tgcgctcgct
     6301 ttccgaacgt gcataaggta gtctgcgcgc gtcctggtcc caccagcaaa gccgactgcc
     6361 tgaacaacgt gctggacgcc atcacccagt ttgaacgtag cgccaatttc gcttttgctg
     6421 gttttattct gcatgacgcc gaagatgtga tttcaccgat ggaattgcgt ctgttcaact
     6481 atctggtcga gcgtaaagat ctgattcaga tcccagtgta tccgtttgaa cgcgaatgga
     6541 cgcacttcac cagcatgact tacattgatg agttttcaga actgcatggc aaagatgttc
     6601 cggtgcgtga agccctcgcc ggacaagtgc ccagcgcagg cgtcggcacc tgtttcagcc
     6661 gccgcgccgt gaccgcactg ttagctgacg gtaacggtat tgctttcgac gtgcagagtc
     6721 ttactgaaga ttacgacatt ggcttccgcc tgaaagaaaa aggtatgacg gaaatttttg
     6781 tccgttttcc ggtggtggac gaagccaaag aacgcgagca gcgtaaattt ttacagcacg
     6841 cacggacgtc aaacatgatc tgcgtgcgcg aatatttccc cgataccttt tcgactgcgg
     6901 ttcgacaaaa atctcgctgg atcatcggca ttgttttcca gggctttaaa acccataaat
     6961 ggacctccag cctgacgctg aactactttc tctggcgcga ccgcaaaggg gcaatcagta
     7021 actttgtcag cttcctcgcg atgctggtga tgatccagct tttgctgttg ctggcgtatg
     7081 aaagtttgtg gcccgatgcc tagcatttcc tttctatttt tagcggcagc gcatggttaa
     7141 tgaccctgct gtggctaaac tttggcttga tggttaaccg catcgtgcag cgagtgattt
     7201 tcgtcactgg ctactacggc ctcacgcagg gcctgctttc cgtcctgcgt cttttctggg
     7261 gcaacctgat taactttatg gctaactggc gcgtgctgaa acaggttctt cagcacggcg
     7321 atccgcgtcg cgtcgcgtgg gataaaacaa cgcatgactt ccccagcgtc actggcgata
     7381 cccgctcgtt gcgcccgtta ggtcaaattc tgctggaaaa tcaggtcatc actgaagaac
     7441 aactcgatac agcactgcgt aatcgcgtcg aaggtctacg cctgggcggt tcaatgctga
     7501 tgcaggggct gattagcgcc gagcagctgg cacaggcgct ggcagagcaa aacggcgtgg
     7561 cgtgggaatc catcgatgcc tggcagatcc cgtcctcgct gattgccgaa atgccagcct
     7621 ccgtagcgct gcattatgca gtactgccgc tgcgtctgga aaatgatgag ttaattgtcg
     7681 gcagtgaaga tggtattgac ccggtttcgc tggcggccct gacgcgtaaa gtcggacgca
     7741 aagtgcgtta cgtcattgtt ctgcggggac aaattgtcac agggttacgc cactggtatg
     7801 cacgccgacg cggtcacgat ccgcgggcaa tgttgtacaa tgcggttcag catcagtggc
     7861 tcacggaaca gcagaccggt gaaatctggc ggcaatatgt gccgcatcag ttcctgttcg
     7921 ccgaaatact gaccacgctc ggtcatatta atcgttcagc aattaacgtg ttgttattgc
     7981 gccatgaacg cagttctctg ccgctcggca agtttttggt caccgaaggc gttatcagcc
     8041 aggaaacgtt ggatcgcgtc ctgacaattc aacgcgaatt acaagtttcg atgcaatcac
     8101 tattactcaa agcaggttta aacacagaac aggttgcgca actggagtcc gaaaatgaag
     8161 gagaataacc ttaatcgcgt catcggatgg tctggtttac tgctgacgtc tttattgagt
     8221 accagcgcac tcgcagacaa tatcggcacc agcgcagaag agctggggct gagcgattat
     8281 cgccattttg ttatttatcc ccgtctcgat aaggcgctga aggcacagaa aaataacgac
     8341 gaagcaaccg ccatccgcga atttgaatat atacaccaac aggtgccgga taatattccg
     8401 ctgactttat accttgcgga agcctatcgt cattttggtc atgatgaccg ggcgcggctg
     8461 ttgcttgagg atcaactgaa acgtcaccca ggagatgccc gacttgagcg cagtctggcg
     8521 gctattccag ttgaagtgaa aagcgttacg actgttgaag aactgcttgc ccagcaaaaa
     8581 gcgggcgacg ctgcgccgac cctgcgttgt cgcagtgaag tcgggcagaa taccctgcgg
     8641 ctggcacaat tacctgtcgc cagagcgcaa ctgaacgatg cgacgtttgc tgcatcgccg
     8701 gaaggaaaaa cgctgcgaac cgatctgctg caacgggcaa tctacctgaa acaatgatcc
     8761 caggcagata cgctatacaa tgaagcacgc cagcagaaca cattaagcgc ggcagaacgc
     8821 cgtcagtggt ttgacgtgct tcttgccggg cagctggacg atcggatcct ggcactgcaa
     8881 tcacagggga tcttcactga tcctcagtca tatattactt acgcgaccgc gctggcttat
     8941 cgtggcgaaa aagcacgcct ccagcattat ctcattgaaa ataagccgct gtttaccacg
     9001 ggcgcacaag agaaaagttg gctctatctg ttatctaaat acagcgccaa ccccgttcag
     9061 gcattggcga attatacggt gcagtttgcc gataaccgcc agtatgttgt tggcgcgacg
     9121 ctaccggtgc tgttaaaaga aggtcagtac gacgcagcgc aaaaactgct cgccaccctc
     9181 cccgccaatg aaatgcttga ggagcgttat gctgtcagcg tggcgacccg taacaaggct
     9241 gaatctctgc gtctggcacg attgctgtat cagcaagaac cgacaaatct tccccgcctg
     9301 gatcaactga cctggcaact gatgcagaac gagcagtcac gcgaagctgc cgatttattg
     9361 ctgcaacgct atcctttcca gggcgatgcg cgtgtcagcc agactttaat ggcgcgactg
     9421 gcgtctctgc tggaaagtca tccttaccta gcaacgccgg cgaaggtggc gattttatcg
     9481 aaacccttac cgctggcgga gcaacgtcag tggcaaagtc agttgccggg tattgcagat
     9541 aattgcccgg caatagttcg cttgctgggc gatatgtcgc cttcctacga tgctgccgcc
     9601 tggaaccgtc tggcaaagtg ttatcgggac acgctacccg gtgtggcgtt gtatgcatgg
     9661 cttcaggccg aacaacgaca accgagcgcc tggcaacatc gtgcggtagc ctatcaggcg
     9721 tatcaggttg aggactacgc caccgcactg gcggcctggc agaaaatcag tcttcacgac
     9781 atgagtaatg atgatctgct cgccgccgcc aacaccgctc aggcagcggg aaatggcgcg
     9841 gctcgcgatc gctggcttca acaggcagaa caacgtgagc tgggaaacaa tgctcaatac
     9901 tggtggctgc atgcgcgacg ttatatccca gctcagccag aactcgcact gaacgatctc
     9961 acgcgctcaa tcaatattgc cccttctgcc aactcttacg ttgcgcgggc gacaatttat
    10021 cgccaacgtc ataatgttcc ggccgcggtg agtgatttgc gcgccgcgct ggaactggaa
    10081 ccaaataata gcaacaccca ggcagcgctt ggttacgcct tgtgggatag cggtgatatc
    10141 gcacagtcgc gggaaatgct cgaacaggcg cataaagggc ttccggacga tccggcactg
    10201 atccgacaac tggcctacgt gaaccagcgt ctggatgaca tgcctgcgac gcagcactac
    10261 gcccggctcg tgattgatga cattgataat caggcataac cccactgacc ccagagcaaa
    10321 atcagcaacg cttcaatttc cgccgtttgc atgaggaggt cggtcgccgc tggacgttca
    10381 gtttcgattc ttccatcggc ttgcgttccg gcgcaatgag taccgctaac aataatgtcg
    10441 gcggtgcagc gcctgggaaa agctatcgta gctacggaca actggaagcc gagtaccgca
    10501 tcggacgcaa tatgctgctg gaaggcgacc tgctctcagt ttatagccgc gtctttgccg
    10561 ataccggaga aaacggggtg atgatgccgg tgaaaaatcc gatgtccggc accggtctgc
    10621 gctggaagcc gctgcgcgat cagatctttt tcctcgccgt cgaacagcag ttgccgctga
    10681 acgggcaaaa tggcgcatcc gataccatgc tgcgcgccag cgcctcattc tttaatggcg
    10741 gcaaatacag cgacgaatgg cacccgaacg gttcaggctg gtttgcccaa aacctgtacc
    10801 tcgatgcggc gcaatatatc cgccaggata ttcaggcgtg gacggcagat tatcgcgtca
    10861 gctggcatca gaaggtagct aacggacaga ctattgagcc ttacgctcac gttcaggaca
    10921 acggctatcg tgataaaggc actcagggcg cgcagcttgg cggtgtcggg gtccgctgga
    10981 atatttggac cggcgagacg cactacgacg cctggccgca caaagtcagt ctcggcgtcg
    11041 agtatcaaca tacctttaag gcgattaatc aacgtaacgg agagcgcaac aacgcgtttc
    11101 tcaccattgg agtgcactgg taaatgcgta agttcatttt cgtattgctg acactgcttt
    11161 tggtcagccc tttttccttt gcgatgaaag gtattatctg gcaaccacaa aaccgagata
    11221 gtcaggttac cgatacccag tggcaggggc tgatgagtca gttacgtttt caaggcttcg
    11281 ataccctggt tttgcaatgg acccgttacg gcgatgcatt tacccagcca gaacagcgca
    11341 cgttattgtt taagcgtgcc gcagctgcgc aacagactgg cctgaagctg attgtcgggc
    11401 tgaacgccga tccggaattt tttatgcacc agaaacagtc gtctgcagcg ctggaaagct
    11461 atcttaatcg cctgctggct gccgatctcc agcaagccag attatggagc gccgcgcctg
    11521 gcataacgcc ggatggctgg tacatcagcg cggaaattga cgacctgaac tggcgcagcg
    11581 aagccgcccg tcaacctttg ctaacatggt taaacaacgc gcagcggctg attagcgatg
    11641 tttcagcaaa accggtttat atcagtagtt ttttcgccgg aaacatgtcg cccgatggct
    11701 atcgccaact gctggaacaa gttaaagcaa ccggagttaa tgtctgggta caggatggca
    11761 gcggcgtgga taaacttacc gctgaacagc gtgaacgtta tctacaggcc agcgccgatt
    11821 gccaaagctc cgccccagca agcggcattg tttatgaact gtttgtcgcc ggcaaaggca
    11881 aaacctttac agcgaaaccg aaaccggacg cagaaattgc ctcgctgtta gcgaaacgtt
    11941 cctcttgcgg taaagacatg ctctatttct ctctgcgcta tttgcccgtc gcgcacggca
    12001 ttctcgagta ttaaatcccc tccaggtaag tcgggtacga cctggcttac ctctttcgct
    12061 ctcgtcagaa ttccatcaag attaattgct aaaaagcatg ctaaagaaag aagatcgtta
    12121 tcggcgaacg ggccgatcac tcatgcagga agcatagatc ctttgagcgg agtttctgat
    12181 atctgaggag tgcgaaatgc aattgagcag cagtgaacct tgcgtggtga tcctgaccga
    12241 aaaagaggta gaggtaagcg tcaataacca tgctacgttt acccttccga aaaactacct
    12301 ggccgccttc gcgtgcaaca ataacgtcat tgaactctca acgttaaatc acgtattaat
    12361 cacccacatc aaccgtaaca tcatcaacga ttatctgttg tttttaaata agaaattaac
    12421 ctgtgtaaag ccctggtcgc ggctggcaac cccggttatc gcttgtcata gccgtacacc
    12481 ggaagtgttc cggctagcca ccaaccacag caagcagcaa tccagcaaac cctgcgaagc
    12541 ggagttgacg cgcgcattac tttttaccgt attgtctaac tttcttgagc aatcgcggtt
    12601 tattgcccta ctgatgtata tcttacgcag cagcgtccgc gacagcgtct gccgcattat
    12661 tcaaagcgat attcagcatt actggaatct gcgaattgtc gccagttcgc tatgtttaag
    12721 ccccagcctg ctcaaaaaga aattaaaaaa cgaaaatacc agctatagcc agattgtcac
    12781 agagtgtcgt atgcgttacg ccgtacagat gttattgatg gataacaaaa atatcactca
    12841 ggtggcgcaa ttatgtggct atagcagcac gtcgtacttt atctctgttt ttaaggcgtt
    12901 ttacagcctg acaccgttga attatctcgc caaacagcga gaaaaagtga tgtggtgaag
    12961 ggcaaagcgg aaacggataa gacgggcata aatgaagaag aaatggcgcg ccctgcagga
    13021 ttcgaacctg cggcccacga cttagaaggt cgttgctcta tccaactgag ctaagggcgc
    13081 gttgataccg caatgcggtg taatcgcgtg aattatacgg tcaacccttg ctgagtcaat
    13141 ggcttttgat ctggttgctg aacaagtgaa cgaccgcgtc tgattttctg atttatttcg
    13201 ctatagcggc aaacaaacgc acaccgctgc gcgtctgaat caagaaaacc cgtattttca
    13261 tgtatcaaag tacaatttac cgccctaacg gaaaattgtc cgctcctatg agactggtaa
    13321 ctatgaaacc aacgtcggtg atcattatgg atactcatcc tatcatcaga atgtctattg
    13381 aagttctgtt gcaaaaaaac agtgaattgc agattgtcct gaaaacggat gattaccgca
    13441 taaccatcga ttatctccga acccgtcctg ttgatttaat cattatggat atagacttgc
    13501 ccggaacaga cggttttacc ttcctgaa
//
LOCUS       DAJTQY010000101        12905 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.101, whole genome shotgun sequence.
ACCESSION   DAJTQY010000101 DAJTQY010000000
VERSION     DAJTQY010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12905
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.101"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(128..1579)
                     /gene="uxaB"
                     /locus_tag="OO349_003198"
     CDS             complement(128..1579)
                     /gene="uxaB"
                     /locus_tag="OO349_003198"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HCR5868170.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(1806..3223)
                     /locus_tag="OO349_003199"
                     /pseudo
     CDS             complement(1806..3223)
                     /locus_tag="OO349_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
     gene            complement(3377..3721)
                     /locus_tag="OO349_003200"
     CDS             complement(3377..3721)
                     /locus_tag="OO349_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HCR5868171.1"
                     /translation="MQYLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVV"
     gene            complement(3721..4647)
                     /gene="glsB"
                     /locus_tag="OO349_003201"
     CDS             complement(3721..4647)
                     /gene="glsB"
                     /locus_tag="OO349_003201"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HCR5868172.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINTGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(4711..5646)
                     /locus_tag="OO349_003202"
     CDS             complement(4711..5646)
                     /locus_tag="OO349_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001580861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HCR5868173.1"
                     /translation="MRGAVPIILAGNGYLLKHAPNVMGCAQLIAQVFKDAGIPQGVYG
                     WLNADNDGVSQMIKDSRIAAVTVTGSVRAGAALKKCVLELGGSDPFIVLNDADLELAV
                     KAAVAGRYQNTGQVCAAAKRFIIEEGIASAFTERFVSAAAALKMGDPRDEENALGPMA
                     RFDLRDELHHQVEKTLAQGARLLLGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGP
                     VAAITIAKDAEHALELANDSEFGLSATIFTTDETQARQMAARLECGGVFINGYCASDA
                     RVAFGGVKKSGFGRELSHFGLHEFCNIQTVWKDRI"
     gene            5726..6841
                     /gene="yneK"
                     /locus_tag="OO349_003203"
     CDS             5726..6841
                     /gene="yneK"
                     /locus_tag="OO349_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HCR5868174.1"
                     /translation="MVTPVSISNYISLPDDFPARNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNTNSATKKIFNNLDNLSYIQRTSFRGNDTLYNEKVQFKLTYPVKNGRHKENI
                     EFQVVINLSPIYLDNFRHDGEINIFCAPNPKPVTMGRVFQTGVERVLFLFMNDFIEQF
                     PMINLGAPIKRAHTPHIEPLPPDHHTAADYLRQFDLLVLNFISRGNFVILPRLWNNSE
                     VHRWFVNKDPNLITAILDITDSELKEDLLQSLMDSLGSNKHVQPEVCICFLSLLAEQE
                     SPHFQDLFLFFANMLLHYHQFMNPNESDLNDVLMPASLSDDKIIKHMARRTLKLFVKN
                     ETPPKVTHEDLVKNRPRSPVRPPIPATAKTPDLPERH"
     gene            6991..8181
                     /gene="ydeA"
                     /locus_tag="OO349_003204"
     CDS             6991..8181
                     /gene="ydeA"
                     /locus_tag="OO349_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HCR5868175.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALVTLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(8206..8871)
                     /gene="marC"
                     /locus_tag="OO349_003205"
     CDS             complement(8206..8871)
                     /gene="marC"
                     /locus_tag="OO349_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HCR5868176.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            9083..9517
                     /gene="marR"
                     /locus_tag="OO349_003206"
     CDS             9083..9517
                     /gene="marR"
                     /locus_tag="OO349_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HCR5868177.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAEICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            9537..9920
                     /gene="marA"
                     /locus_tag="OO349_003207"
     CDS             9537..9920
                     /gene="marA"
                     /locus_tag="OO349_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HCR5868178.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            9952..10170
                     /gene="marB"
                     /locus_tag="OO349_003208"
     CDS             9952..10170
                     /gene="marB"
                     /locus_tag="OO349_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HCR5868179.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(10201..11100)
                     /gene="eamA"
                     /locus_tag="OO349_003209"
     CDS             complement(10201..11100)
                     /gene="eamA"
                     /locus_tag="OO349_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HCR5868180.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVVSLIFDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVGS"
     gene            11364..12482
                     /gene="ydeE"
                     /locus_tag="OO349_003210"
     CDS             11364..12482
                     /gene="ydeE"
                     /locus_tag="OO349_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HCR5868181.1"
                     /translation="MATLPFMTIYLSRQYSLSVDLIGYAMTIALTIGVVFSLGFGILA
                     DKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLFFALINCAYSVFATVLKAWFADNL
                     SSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSINLPFWLAAICSAFPMLFIQIWVK
                     RSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLASFVSGAFASCISQYVMVIADGDF
                     AEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRALMTAGTLCFVIGLVGFIFSGNNLL
                     LWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKASYFSAQSLGWLGAAINPLVSGVVL
                     TSLPPSSLFVILALVIIAAWVLMLKGIRARPWGQPALC"
     gene            12609..12704
                     /gene="mgtS"
                     /locus_tag="OO349_003211"
     CDS             12609..12704
                     /gene="mgtS"
                     /locus_tag="OO349_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HCR5868182.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
BASE COUNT         3399 a         3296 c         2950 g         3260 t
ORIGIN      
        1 gtcaagctta caacattaac aggcgctgca tgtgacacct gttaatgata aagggatata
       61 ggatgttgta actaacatag tgaaataaga aacccggtcg aaaccgggtt cagaagtaac
      121 ggtgttatta gcacaacgga cgtacagctt cgcgcatccc tttttcaaga atcgcatcca
      181 ggtcattagc aacctgctcg accagaccag gcacttgcgt caggtcctgc tcccagtggt
      241 ctttttctgc caacacaatc gctaccagtt cctgggtacc gataacacgg tcacgatgct
      301 ggctccagag ttgctgataa cgttccagcc agtgtgcatc gtcctgtacc ggatatgttt
      361 ctccgttacg ctcaccacga tagaacgcaa tcaatgccgc taatgcgaaa gtaaggcgcg
      421 ccggaagtgt gccatttgcc ttctgccctg ccagcagctg cggcaggatg cgggtgcgga
      481 acttggtcat accgttgagc gcgatagaca gcagctgatg cttaatgtac gggttacgga
      541 aacgcccggt gactgcactg gcgaaagatt ccagttcatc acgaggcaaa tccagtaccg
      601 gaataatttc ttcgtagatg gctttttcaa cgaatgcgca aatttcagca tcgttcatcg
      661 cttcacctac ggtatccagc cctgcctgga acgccaccgg caccagcgcg gtgtgcgcac
      721 cgttgaggat tgccactttg cgttctttat acggtttaat atcgtcaaca atcagcacgt
      781 tgagcggata tttgtccagg cgcagctcag tcgctaagga tttcggcccc tgaatcacaa
      841 acaggtaaaa gtgttcagcg gtgtcgagga aaccatcgtg ataacccagt tcttcttcca
      901 gtttagccac ttcatcgcgc ggataaccgg taacgatacg gtccaccagc gtagaacaga
      961 agctgttagc ctgatccagc cattgaataa atgcttctgg cagtgcccac tcttgcgcat
     1021 agcgcagcac cagttcacgc aacgcgtcgc cattgtagtc aatcaactca cacggaatga
     1081 tgatccaacc tttatccagc gcaccgttga aatggctaaa gcgttcgaac agcagacggg
     1141 tcagttttgc cggatagctt accgctggcg cgtcatcgaa tttatcgccc gcatggtagc
     1201 tgatacccgc ttcggtggtg ttagagaaaa caaagcgcat ttccgggttg tgcgccagtt
     1261 tcaggaattc atcgtattca ctgtagacgc tgatttcacg attaaccgag cgaatcagac
     1321 gcgcgtcgct gaccgcttcc cccttctcat tcaggccacg gataatggtg gtgtacagac
     1381 catcctgcgt gctcagtgac ggcgggaatg aagtttcaat cggacgaaca acgaccacgc
     1441 cagaattcag atcggtgtgc tcattcagga gatcgatttg ccagtcaaca aaggcgcgca
     1501 ggaagttacc ttcaccaaac tgaatgatac gttctggata ctgtgcaccg ggaaaatcgc
     1561 gacgatttag tgttttcaca atgggttccc ttctgattag tcatacaacc tgtttgaatt
     1621 ggtgcgacag gttagcaaac tttaatacgc cgaacccctg ttttgatcaa ctcctgatga
     1681 ttaatgagca gttttatgag aaaagtgtgg cgcggatcat ggtttaatcg aggaaatacg
     1741 ccttttcctg gatcataaag tggtagaaca cattgcattc aaatcgcgcg gaatgaataa
     1801 agatgtcaga caacttcctc accgtaacgc atagtgctgg tacggttgcg cccatctttc
     1861 ttcgagcgat acagacacgt atcggcttcg accatcaatt tattaaatca tcggtcagcg
     1921 ttcggtacga tgcgcgacca ctaccgacgc caatacttac cgtgagataa agcgtttttt
     1981 gttgccaggt gaatggttgc agttcaacgc ctttacggat tttttccgcc attagcagac
     2041 catcgacagg attcaccgac ggcactgcaa cagcaaattc ttcgccgccc atgcgcgcca
     2101 ccagcccctt atcaccgaca atcttctgaa tatgccgggc aaacacgctt aacactttat
     2161 cgccacattc atgtccatag ttatcgttaa tgcttttgaa atagtcgata tcaagcagca
     2221 tgaccgtcag atgttgtgtc tgtttcagcg atggactttt cagcgcctca taaagaccgg
     2281 accgtgagta aacctgagtc agaaaatcaa agtcggctcg cagcgcaact tgcttcatta
     2341 gcaaattgat cgctgccacg ctaaaagaaa ccataattgg gcatatcgcc atcgtggcaa
     2401 taccgagacg tgcggagaac atttccggaa tggagaacgg cgaaccgacc gagatatcaa
     2461 tcaccgaatt tgccaccagt acgatttcca ccgcaccggt gacaaaggtc aacagacatg
     2521 ttacctgcgg cgtatagcgc actgcacacc agattaatgc aggcagcggg aacgccagac
     2581 tccccgctcc gccaatgact accgaggcaa taaccgacac aataagcgcg atggcgggca
     2641 tcatctgttc tgctttaaag cgcggcagca ctccaggaat agccaacgtc agcatacaag
     2701 gcacgatcaa cacgcccgtt gagaattgct cactgaacca gtccgcaagc aaaggccaga
     2761 aatccagact atcaatactg accgaaccaa tcgccccgac aatagcgcat aataatgcgg
     2821 caatcagaca gtaattaaat agccgtaaag cacttactgg ctcatactta tttttcccca
     2881 gacgcttatc ccgcacgacc agtaaggcga cggtaacaat aaacatcata ttggagaaat
     2941 tgataaccag tgaaaccagt ccccattcgg tggttatggc atcataaacc agcatcgcca
     3001 cataactgat cgcatagtaa tgcagtcgat taagccagac atagcgggca aacacccccg
     3061 ccattacgcc attcagcggc cagaaaagag aaagttcgtg aaccagccgt aactctgcac
     3121 caataaaata gaacagcgtg gttagcgcaa aaatagcgat gctattacgt agcagatgac
     3181 cttcctggaa caaacggaaa gttgaaatgg ggtgtacatg cattaattat ctgtccatac
     3241 aattgcgggt ggcaattatc tcaacgtgat aagtaaattc tcataaagga tagcatgttc
     3301 gctaacagcg gcggcaaata aaggtaccct gggaatgcta ttttatcgtt ttccgcaatt
     3361 ctcatggctg attcatctat acaaccagcc agtgatagat taccgccacg atataacgtt
     3421 gttgttcttc gcttaacgaa acaccctgcg gaatgttatg ccacttagcc agacaaccgc
     3481 gacagcaggt cgcggtggcg tgctgagcga taaacaccgg atgcccgcgc atgggggttt
     3541 gcttaccgtc attagccggt aatgccggag caagacgttt agcgacaaaa tccgccgcat
     3601 gttgttcgat gactggcgcg cctttctcca ggcaatactg acgctctttc atgcccagac
     3661 gaaagcgaga gcgaaatttt gaacgggata aacgcgcgaa gagtggatcg agatactgca
     3721 ttaataaacc gaacgcccta actgtttcgt caattgttca aggacggcga tacccgcaag
     3781 cgagttacct gcatcatcca gttccggact ccagactgcg atggccattt catgcggaac
     3841 aatcgccaca ataccgccac caacgccaga tttcgccggt agtcccaccc gccaggcaaa
     3901 ctcccccgcg ttctggtaca taccactggt cgccatcagc gcgttaattt gccgcgcctg
     3961 cattggcgtc accacaggtt catcaatatg aatagctttc ccctgattag ccagaaagac
     4021 aaacgtccgg gccagctcta cacagctcat tttcagagcg cagtaatgaa agtagttttg
     4081 cagaacggtt gtcacgtcat gatggaaatt gccaaacgac ttcatcagcc aggcgatagc
     4141 cgcatttcgc gcggaatgtt caaattcgga acgcgctacc accgtatcgt atgaaatatc
     4201 agacacaccg cttaagccgc gcacgacttc cagcatacgt tgccgtggtg cgcttagtcg
     4261 cccttgcaac atatcgcaga ccaccagcgc accggtatta atgaacggat tacgcggaat
     4321 accctgttcc atttccagtt gcactaaaga attgaacggt gatccagacg gatctttgcc
     4381 gacgcgttgc cagatttcct cttcggagta atgacgcatg gcgacaacga gactcagcac
     4441 tttggaaata gactgaatgg aaaaacgttc ttgcgcgtct ccggcctgaa aaagctgtcc
     4501 gtcaacggta cagatagcaa tccccaatcg ggaaccgtct actgtagcca gcgccggaat
     4561 ataatccgcg actttaccct gaccaatgag cggccgcact tgccgcaaga tgttttctaa
     4621 aattgcatta tccatggcga ctgccacttt ctactcctgg accgcaggtc tgaaaagacc
     4681 tgcgagtata tcagagctga atatgtcgcg tcagatccgg tctttccaca ctgtctggat
     4741 attacagaat tcgtgtaagc cgaaatggga aagctcacga ccaaagccac tctttttcac
     4801 gccaccaaag gccactcgcg cgtcgctggc acaataacca ttgataaaca ccccaccgca
     4861 ttccagacgt gccgccatct gtctggcctg tgtttcgtcg gtggtaaaaa tggtcgctga
     4921 aaggccgaac tcactatcat tagccagttc cagcgcatgt tctgcatctt tcgcaatggt
     4981 gattgcagca acggggccaa acatttcttc ccgaaacgcg gtcatttctg gggtaacatt
     5041 cgccagaacc gttggcggat agtagttacc tgctccagcc atcttttccc cgcccagtaa
     5101 caaacgcgca ccctgcgcca gggttttctc cacctgatga tgcagctcat cacgtaaatc
     5161 aaaacgagcc attggtccga gagcgttctc ttcatcacgg ggatcgccca ttttcaaggc
     5221 tgccgcagct gacacaaaac gttcggtaaa tgccgaagca attccctctt cgataataaa
     5281 gcgttttgcc gctgcacaaa cctgtccggt attctgataa cgtccggcta ccgccgcttt
     5341 aaccgccagt tccagatcgg catcgttaag cacaataaat ggatccgaac cgcccagttc
     5401 cagtacgcat tttttcagtg ccgctccagc acgaacactt ccggtcaccg tgacagcagc
     5461 aatgcgcgag tctttaatca tctgactgac accgtcgttg tcggcattca gccagccata
     5521 tacgccttgc gggattcccg catctttaaa cacttgggca atgagctgag cacagcccat
     5581 cacattcggc gcatgtttaa gtaagtagcc gttacctgca agaatgatgg gaacagcgcc
     5641 acgcatcacc tgcctttgat agttcttgca gctcaaatgc cctatatagt gatgttccga
     5701 tgacttatga ctatatgggg caaatatggt tacgccagta agcatcagca attacatatc
     5761 tcttcctgat gattttcccg ctcgtaatat tgcacctcaa gtaaaagaag ttttaaagga
     5821 ttttattgat gcactcagta ccataatatg tgatgaagag tggcgcacgt ctttaaacac
     5881 caactcagcc acaaaaaaga tatttaacaa ccttgataac ttatcatata ttcagaggac
     5941 ttcttttcgg ggtaacgaca cactatacaa tgaaaaggtt cagtttaaac tcacttatcc
     6001 tgtcaagaat ggaagacaca aagaaaatat tgaatttcag gtagtaataa atttaagccc
     6061 tatttattta gataattttc gccatgatgg agaaattaat attttttgcg ctcccaaccc
     6121 aaagcctgtc actatggggc gcgtatttca gaccggcgtc gagcgtgtgc tctttctgtt
     6181 tatgaatgat tttattgaac aatttccaat gatcaacctt ggcgccccca tcaaaagagc
     6241 gcatacacca catattgaac ccctgcctcc ggatcaccat accgctgcag attacttacg
     6301 ccagtttgat ttgcttgtcc tgaattttat ctctcgcggt aattttgtca tactcccccg
     6361 attatggaat aactctgagg ttcacagatg gttcgtcaat aaagatccta acttgatcac
     6421 cgccattctc gacataacgg acagcgaatt aaaagaggat ttattgcaaa gcctgatgga
     6481 ttcattaggt tctaacaaac atgtacaacc cgaagtctgc atctgcttct tatccctttt
     6541 agcagaacaa gaatctcctc attttcagga cttgttttta tttttcgcca atatgttatt
     6601 gcactatcac caatttatga atcccaatga aagtgatctg aatgacgtgt tgatgccagc
     6661 atcattaagt gatgataaaa ttatcaaaca tatggcgcgc aggaccctca aactgtttgt
     6721 aaaaaatgaa acgccgccaa aagttactca cgaagacctg gtgaaaaaca ggcctcgctc
     6781 ccctgtcaga ccacctatac ccgcaaccgc caaaacgcca gacctccctg aacgtcatta
     6841 aaccgtgatg ttaccgactc tctgactcgg aacagaatca gcgtcagaga aacggaaaac
     6901 gcgatccaga tcacaaatgc attgtattca catcattaac cgttttaaga tcatttcatc
     6961 actttttcgc aactcacccg ataatctgtt atgacaacaa acactgtttc ccgcaaagtg
     7021 gcgtggctac gggtcgttac gctggcagtc gccgccttca tcttcaacac caccgaattt
     7081 gtccctgttg gcctgctctc tgacattgcg caaagttttc acatgcaaac cgctcaggtc
     7141 ggcatcatgt tgaccattta cgcatgggta gtagcgctaa tgtcattgcc ttttatgtta
     7201 atgaccagtc aggttgaacg gcgcaaatta ctgatctgcc tgtttgtggt gtttattgcc
     7261 agccacgtac tgtcgttttt atcgtggagc tttaccgttc tggtgatcag tcgcattggt
     7321 gtggcttttg cacatgcgat tttctggtcg attacggcgt ctctggcgat ccgtatggct
     7381 ccggccggga agcgggcaca ggccttgagt ttaattgcca ccggtacagc actggcgatg
     7441 gtcttaggtt taccgctcgg gcgcattgtg gggcagtatt tcggctggcg aatgaccttc
     7501 ttcgcgattg gtattggggc gcttgttacc cttttgtgcc tgattaagtt acttccctta
     7561 ctgcctagtg agcattccgg ttcattgaaa agcctcccgc tattgttccg ccgcccggca
     7621 ttgatgagca tttatttgtt aactgtggtg gttgtcaccg cccattacac ggcatacagc
     7681 tatatcgagc cttttgtgca aaacattgcg ggattcagcg ccaactttgc cacggcatta
     7741 ctgttattac tcggtggtgc gggcattatt ggcagcgtga ttttcggtaa actgggtaat
     7801 caatatgcgt ctgcgttggt aagtacggcg attgcgctgt tgctggtgtg tctggcactg
     7861 ctgctacctg cggcgaacag tgaaatacac ctcggggtgc tgagtatttt ctgggggatc
     7921 gcgatgatga tcatcgggct tggtatgcag gttaaagtgc tggcgctggc accagatgct
     7981 accgacgtcg cgatggcgct attctccggc atatttaata ttggaatcgg ggcgggtgcg
     8041 ttggtaggta atcaggtgag tttgcactgg tcaatgtcga tgattggtta tgtgggcgcg
     8101 gtgcctgctt ttgctgcgtt aatatggtca atcattatat ttcgccgctg gccagtgaca
     8161 ctcgaagaac agacgcaata gttgaaaggc ccattcgggc cttttttaat ggtacgtttt
     8221 aatgatttcc aggatgccgt taataataaa ctgcacaccc atacatacca gcaggaaccc
     8281 catcaggcgg gagatcgctt caatgccacc cttgcccacc agccgcataa ttgcgccgga
     8341 gctgcgtagg cttccccaca aaataaccgc caccaggaaa aagatcagcg gcggcgcaac
     8401 catcagtacc caatcagcga aggttgaact ctgacgcact gtggacgccg agctaataat
     8461 catcgctatg gttcccggac cggcggtact tggcattgcc agtggtacaa aggcaatatt
     8521 ggcactgggt tcatcttcca gctcttccga cttgcttttc gcctccggtg aatcaatcgc
     8581 tttctgttgc ggaaaaagca tccgaaaacc gataaacgcg acgattaagc cgcctgcaat
     8641 tcgcagaccg ggaatcgaaa tgccaaatgt atccatcacc agttgcccag cgtaatacgc
     8701 caccatcatg atggcaaata cgtacaccga ggccatcaac gactgacgat tacgttcggc
     8761 actgttcatg ttgcctgcca ggccaagaaa tagcgcgaca gttgttaatg ggttagctaa
     8821 cggcagcaac accaccagcc ccaggccaat tgctttaaac aaatctaaca ttggtggttg
     8881 ttatcctgtg tatctgggtt atcagcgaaa agtataaggg gtaaacaagg ataaagtgtc
     8941 actctttagc taaccttgca tcgcattgac caaaacttga accgatttag caaaacgtgg
     9001 catcggtcaa ttcattcatt tgacttatac ttgcctgggc aatattaccc cctacaacta
     9061 attacttgcc agggcaacta atgtgaaaag taccagcgat ctgttcaatg aaattattcc
     9121 attgggtcgc ttaatccata tggttaatca gaagaaagat cgtctgctta acgagtatct
     9181 gtctccgctg gatattaccg cggcacagtt taaggtgctc tgctctatcc gctgcgcggc
     9241 gtgtattact ccggttgaac tgaaaaaagt gttgtcggtc gacctgggag cactgacccg
     9301 tatgctggat cgcctggtct gtaaaggctg ggttgaaagg ttgccgaacc cgaatgataa
     9361 gcgcggcgta ctggtaaaac ttaccaccag cggcgcggaa atatgcgaac aatgccatca
     9421 attagttggc caggacctgc atcaagaatt aacaaaaaac ctgacggcgg acgaagttgc
     9481 aacacttgag catttgctta agaaagtcct gccgtaaaca aaaaagaggt atgacgatgt
     9541 ccagacgcaa tactgacgct attaccattc atagcatttt ggactggatc gaggacaacc
     9601 tggaatcgcc actgtcactg gagaaagtgt cagagcgttc gggttactcc aaatggcacc
     9661 tgcaacggat gtttaaaaaa gaaaccggtc attcattagg ccaatacatc cgcagccgta
     9721 agatgacgga aatcgcgcaa aagctgaagg aaagtaacga gccgatactc tatctggcag
     9781 aacgatatgg cttcgagtcg caacaaactc tgacccgaac cttcaaaaat tactttgatg
     9841 ttccgccgca taaataccgg atgaccaata tgcagggcga atcgcgcttt ttacatccat
     9901 taaatcatta caacagctag ttgataacgt gacaacgtca ctgaggcaat catgaaacca
     9961 ctttcatccg caatagcagc tgcgcttatt ctcttttccg cgcagggcgt tgcggaacaa
    10021 accacgcagc cagttgttac ttcttgtgcc aatgtcgtgg ttgttccccc atcgcaggaa
    10081 caaccaccgt ttgatttaaa tcacatgggt actggcagtg ataagtcgga tgcgctcggc
    10141 gtgccctatt ataatcaaca cgctatgtag tttgttctgg ccccgacatc tcggggctta
    10201 ttaacttccc acctttaccg ctttacgcca ccgcaagcca aatacattga tatacagccc
    10261 ggtcataatg agcaccgcac ctaaaaattg cagacccgtt aagcgttcat ccaacaatag
    10321 tgccgcactt gccagtccga ctacgggcac cagtaacgat aacggtgcaa cccgccaagt
    10381 ttcatagcgt cccagtaacg tcccccagat cccataacca acaattgtcg ccacaaacgc
    10441 cagatacatc agcgacaaga tggtggtcat atcaatagta accagactgt gaatcatgga
    10501 tgcggaacca tcgaaaatca gcgagacaac aaagaaggga atgattggga ttaaagcgct
    10561 ccagattacc agcgacatca ccgccggacg cgttgagtgc gacatgatct ttttattgaa
    10621 gatgttgcca cacgcccaac taaatgctgc agccagggtc aacataaagc cgagcatcgc
    10681 cacatgctga ccgttcagac tatcttcgat taacaccagt acgccaaaaa tcgctaaggc
    10741 gatccccgcc aattgtttgc catgcagtcg ctccccgaaa gtaaacgcgc caagcacgat
    10801 agtaaaaaac gcctgtgcct gtaacaccag cgaagccagt ccagcaggca taccaaagtt
    10861 aatggcgcag aacagaaagg caaactgcgc aaaactgatg gttaatccat accccaagag
    10921 caaattcagt ggtactttcg gtcgtgcaac aaaaaagatg gccggaaaag cgaccagcat
    10981 aaagcgcaaa ccggccagca tcagcggtgg catgttatga agccccactt tgatgaccac
    11041 aaaatttagc ccccatacga ccactaccag tagcgccaac accccatctt ttcgcgacat
    11101 tctaccgcct ctgaatttca tcttttgtaa gcaatcaact tagctgaatt tacttttctt
    11161 taacagttga ttcgttagtc gccggttacg acggcattaa tgcgcaaata agtcgctata
    11221 cttcggattt ttgccatgcg tccgatggtt aataacaacg acgaggcaag aagggcgctg
    11281 gtagagcgtc gtagggataa gttcatttgt ctgtccggca gtgcgtttcg ttatgtttta
    11341 ttttagagat gtaaagaaat agcatggcta cgctgccatt tatgaccatt tacttgagtc
    11401 gccagtacag cctgagtgtc gatctaatcg gttatgcgat gacaattgcg ctcactattg
    11461 gcgtcgtttt tagcctcggt tttggtatcc tggcggataa gttcgacaag aaacgctata
    11521 tgttactggc aattaccgcc ttcgccagcg gttttattgc cattccttta gtgaataacg
    11581 tgacgctggt tgtgctcttt tttgccctca ttaactgcgc ctattctgtt tttgctactg
    11641 tgctgaaagc ctggtttgcc gacaatcttt cgtccaccag caaaacgaaa atcttctcaa
    11701 tcaactacac catgctaaac attggctgga ccatcggtcc gccgctcggc acgctgttgg
    11761 tgatgcagag catcaatctg cccttttggc tggcagctat ctgttccgcg tttcccatgc
    11821 ttttcattca aatatgggta aagcgcagcg agaaaatcat cgccacggaa acaggcagtg
    11881 tctggtcgcc gaaagtttta ttacaagata aagcactgtt gtggtttacc tgctctggtt
    11941 ttctggcttc ttttgtaagc ggcgcatttg cttcatgcat ttcacaatat gtgatggtga
    12001 ttgctgatgg ggattttgcc gaaaaggtgg tcgcggttgt tcttccggtg aatgctgcca
    12061 tggtggttac gttgcaatat tccgtgggcc gccgacttaa cccagctaac atccgcgcgc
    12121 tgatgacagc aggcaccctc tgtttcgtca tcggactggt cggttttatt ttttccggca
    12181 acaacctgct attgtggggt atgtcagctg cggtatttac tgtcggtgaa atcatttatg
    12241 cgccgggcga gtatatgttg attgaccata ttgcgccgcc aggaatgaaa gccagctatt
    12301 tttccgccca gtctttaggc tggcttggtg ccgcgattaa cccattagtg agtggcgtag
    12361 tgctaaccag cctgccgcct tcctcgctgt ttgtcatctt agcgttggtg atcattgctg
    12421 cgtgggtgct gatgttaaaa gggattcgtg caagaccgtg ggggcagccc gcgctttgtt
    12481 gatttaagtc gaacacaata aaaatttaat tcagccttcg tttaggttat ctctgctaat
    12541 atctttctca ttgagatgaa aattaaggta agcgaggaaa cacaccacac cataaacgga
    12601 ggcaaataat gctgggtaat atgaatgttt ttatggccgt actgggaata attttatttt
    12661 ctggttttct ggccgcgtat ttcagccaca aatgggatga ctaatgaacg gagataatcc
    12721 ctcacctaac cggccccttg ttacagttgt gtacaagggg cctgattttt atgacggcga
    12781 aaaaaaccgc cagtaaaccg gcggtgaatg cttgcatgga tagatttgtg ttttgctttt
    12841 acgctaacag gcattttcct gcactgataa cggatcgttg acacagtagc atcagttttc
    12901 tcaat
//
LOCUS       DAJTQY010000102        12806 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.102, whole genome shotgun sequence.
ACCESSION   DAJTQY010000102 DAJTQY010000000
VERSION     DAJTQY010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12806
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.102"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(<1..128)
                     /locus_tag="OO349_003212"
     CDS             complement(<1..128)
                     /locus_tag="OO349_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR5868183.1"
                     /translation="MRITLQWDINEMSYFYSASTNGFYSTEFHGTNIPDDAVEISES"
     gene            complement(100..891)
                     /locus_tag="OO349_003213"
     CDS             complement(100..891)
                     /locus_tag="OO349_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005061891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5868184.1"
                     /translation="MAINNFKPFALDPNANVTSQADWEALPALLSGFTAGKASSAQVN
                     KAIRQASFIAAALAQYTANKSRLDVLDDGDLNGFISKMGTAFGKDFQALDATLSALAG
                     LATGANKLPYFTGNDTAAQTDLTSVGRDIIGKNTIADILTYLGLGEAAKRNVGTGANQ
                     IPDMSLFASSNTATAAAQKFPSGLILQCGQLNGSPNVSSTYGMRFPMTFSRVLAVTVT
                     LNVTGASGQPTVSATNVHNTGFDITVSPGSGYGSSADAYYIAMGY"
     gene            complement(891..1571)
                     /locus_tag="OO349_003214"
     CDS             complement(891..1571)
                     /locus_tag="OO349_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
                     /protein_id="HCR5868185.1"
                     /translation="MSKYTELITNYHATKPLFFDHIDLSTRPLIDVSSTMSGLITAFD
                     IDTAVGVQLDILGLWIGRSRIVSQPISGVYFSWDTDGLGYDQGIWKGPYDPDSGYTTL
                     SDETYRIILKAKIAINNWDGRNDSLPPILDAATEGSGLRMQIVDNQDMTISVWVFPET
                     DISDVSLELIAAIKQGYLTVKAAGVWAGDVETPSVEAPSEGSKFFGFDMDNEYIGGFD
                     VGAWGTIL"
     gene            complement(1568..2767)
                     /locus_tag="OO349_003215"
     CDS             complement(1568..2767)
                     /locus_tag="OO349_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001197067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HCR5868186.1"
                     /translation="MALNLDTLGLSATVTAQGISAPDYQTILDTLTSYFRQIYGSDAY
                     LEPDSKDGQMVALVALAVHDANNTAIGIYNSFSPTTAQAAALSSNVKINGITRKVATN
                     STADLLLTGTAGTTITNGSARDKNGIIWNFPASVAIGVDGTVLVTATCANSGSVAALA
                     GTITTINTPTRGWVSVTNPVAATVGSPAETDAELRIRQGQSVALPSITPFEGVDGAIA
                     NVAGVTRHKLYENDTGATDSNGLPPHSISAIVDGGDVTEIAQTIRGSKGQGTATYGKT
                     SVTVPDTYGNPHVISFSRSTDVPIFVAITLKVFTGYTSQIGEQIKQAVADYINGLTIG
                     DDVLLSRIYSPANLGVVSGGNARYYDITDLLIGKSSGSVSASNIDIAYDASASCSTAN
                     ISITVTS"
     gene            complement(2768..3121)
                     /locus_tag="OO349_003216"
     CDS             complement(2768..3121)
                     /locus_tag="OO349_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001270645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5868187.1"
                     /translation="MRYRREDTEGDYTFGQGDDTFLIDSPECVAQAVKTRFELWRGQW
                     FLDLTEGTPYVQSVLGKQRSDVYILAIRERIQDTPGVLSILSFDTNYDGTSRRVTFTS
                     SIDTIYGQTTVTSEA"
     gene            complement(3121..3861)
                     /locus_tag="OO349_003217"
     CDS             complement(3121..3861)
                     /locus_tag="OO349_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HCR5868188.1"
                     /translation="MPIPTQSQIGGEQQTAQAIADSVSTQMRVAMPGIIQSFDPETVT
                     CTVEVALRGIVGDGSTELKPLVDVPVIFPRGGGCTLTFPVKEGDECLLIFADRCIDFW
                     WQSGGVQETVDPRQHDLSDAFAIVGPQSQARKISGISTSAAQLRTDDGAAFVEVAAGH
                     NITIKTPGQLTATAEGGTTITSPTITLNGNVTINGNLSQGMGESGGTATMLGPVMVTN
                     DVTASGISVATHKHGGVQTGGGTTGGPQ"
     gene            4084..4374
                     /locus_tag="OO349_003218"
     CDS             4084..4374
                     /locus_tag="OO349_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000506882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HCR5868189.1"
                     /translation="MGFRFRKRIRIAPGLAINISKSGVSTSIGGKGSTINIGKKGVKM
                     TNGLPGTGLSHTTNLYSPGKSTEKKQLTHKQKIIRNILFVIIVFIIIKAQYF"
     gene            complement(4398..4742)
                     /locus_tag="OO349_003219"
     CDS             complement(4398..4742)
                     /locus_tag="OO349_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5868190.1"
                     /translation="MRHLLCTVTVFAVLISAPAFADQQCGDFKIHWADDGLARINGAK
                     PEMQKITFLKNKGDYNNIKMDWRMATDQPGRWVGLEYINRNGKAILNAQWLQASMDSP
                     RQYATYDCRKVK"
     gene            complement(4755..5819)
                     /locus_tag="OO349_003220"
     CDS             complement(4755..5819)
                     /locus_tag="OO349_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001567585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5868191.1"
                     /translation="MSLNWMRHFELQLLDQNGQGVSLSDFKVTFQIEWADTRWPRVAN
                     VKIYNLSTDTTNKILGQEFAKIRIIAGYDGIAPDVDASQVGVSREISPDQIGQVNGQN
                     YGLIFDGDIRFTVTGKDNITDSWVLIQAIGDHEAFLYATTITTLAAGYTVADLHRATM
                     QDFNAFGVTQGITGDFPDTVFPRGRAIYSSSRNVMDNIAAQCKATWQLVDGQVQMVPE
                     DKYIHEAIVLNANTGLIGMPQQTMGGGVNVRCLINPNIRINGLIQLDQASVYRATLGN
                     SEIAQSPGRITETEENGNRVLTGTTSQAASIATDGVYIVKAIDYTGDTRGQAWYMDLM
                     CFARGARDLYSPKAMQGTTN"
     gene            complement(5822..6127)
                     /locus_tag="OO349_003221"
     CDS             complement(5822..6127)
                     /locus_tag="OO349_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006120161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5868192.1"
                     /translation="MQFNEIPLSPDNQQFRVLLGNTTYTLRIIWRDAAGWIMDVMDSG
                     GAALLSGVPLLTGVNLLRQYPQLGIDGALVVATDKGAPDEPTKTNLGTYSHLIFVQE"
     gene            complement(6129..6731)
                     /locus_tag="OO349_003222"
     CDS             complement(6129..6731)
                     /locus_tag="OO349_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006120162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5868193.1"
                     /translation="MDILSAIFRQQSRRIGILIPSVVVSEKHSDALEITEHPVEKPTT
                     NSASGFIADHAYKRPSEVTMECGFAGGGSLLDFIDTSSIGLSAGLSPKETYQQLLDLQ
                     SSRVPFDVVTGKRVYSNMLVRAIEVTTDKTSENVLNCTLTLREVIMSQTQSVSVADKS
                     DMQDGVSTSAVQNSGMKSTTPPNESLLSQLGGSVTSAFGG"
     gene            complement(6731..8746)
                     /locus_tag="OO349_003223"
     CDS             complement(6731..8746)
                     /locus_tag="OO349_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000016305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HCR5868194.1"
                     /translation="MSGNADTIKDFLVSLGFDIDQAGANKFEAVLKGVTANVLKVGAV
                     VKGAALSIVGFTTQIANGLDKIYWASQRTGASVQGIKALGYAASQTGASAESAMSSLE
                     GLAGFMRSNPGAEGFLNRLGVQTRDASGKMRDTAAIFTGVGQKLNNMPYYRAKQYAQM
                     LGIDENTLMAMRRGMGQLSSEYALTAKRIGFNAESAAKQSNIFMTSMRNLTMTLGQAK
                     DKIGSNLAGGLAGSIDNFRRQILDNWPKIEAVITKIIKGILWAGDAITRVLWRTGQAV
                     EGVIAWFKKLNPATQQLIALFSGLLVAWRLLNTAFMSSPLGMITTLIIALGLLLDDYQ
                     TWKEGGKSLIDWGKWKTEIDQAVKMIGDLKKTVTDLTKALAKLLGIDPKSWSLKWDFS
                     NFISQMGEFGKMLNMIADLLNAIKDGRWADAASIGKQMLNQGSENPSAMPMVTDSANG
                     TADWIKEHWGFDPRSVGRTVRGWFGDDEPEQLGQSVKRPQPTKAGSELLGWMQPMFTN
                     LEQLYRLPEGLLRSVAITESGGNQFAVSGAGAKGLFQFMDGTARDMGLRGNDVFDPEK
                     SAQAAAKYLSQLLRQNGGDLSKALASYNWGIGNVKRYGMGLMPQETRNYIPKVMSNMP
                     TSAPVIQQETNINIHGVFDPREAARLTVDRQKGVNSQLTQQLPAGPR"
     gene            complement(8924..9349)
                     /locus_tag="OO349_003224"
     CDS             complement(8924..9349)
                     /locus_tag="OO349_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5868195.1"
                     /translation="MEFEIKGVKYRTAKLSVFEQLKVSRKLLPVLAGMVSDFRSVQEK
                     ISSKDTEGAMATILPKIANAVSDLSDGDVDAILFPCLSVVSREHMKGWVPVCQHGEMA
                     FDDIDLLTMLQLVARVVADSLGNFLQGLPTSETPTPLAE"
     gene            complement(9353..9793)
                     /locus_tag="OO349_003225"
     CDS             complement(9353..9793)
                     /locus_tag="OO349_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3277 family protein"
                     /protein_id="HCR5868196.1"
                     /translation="MATYSFLDVTASLTGPTGVIDLGQGSANSEEGITQTMGGNKNTM
                     TIGADGEVMHSLHADKSGTITVTLLKTSPVNKKLSLAYNAQSQSSATWGNNVIVIRNT
                     ASGDISTARSCAFQKQPDFNNAKEGGTVAWVFDCGKIDQLLGEF"
     gene            complement(9804..10949)
                     /locus_tag="OO349_003226"
     CDS             complement(9804..10949)
                     /locus_tag="OO349_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3383 domain-containing protein"
                     /protein_id="HCR5868197.1"
                     /translation="MAQGLPVSNVVNVDVIMSPRAASGRNFGALLILGPSTIIPVSER
                     IRRYSAAEDIGKDFGVESPEYKAAQVFFSQSPKPQEVFVGRWVKTKGDSEQATPETLE
                     QAVNAMLDYTSWYGLGIADDEDIPDADWLKVAAAIESSSVSRILAITTSDEKCLQTAS
                     SDDLASKLKTAGYSRSFIQYSSGNKYAALSAFGRAFTVNFNGSNTAITLKFKQEPGVG
                     YETLTVSQASALDAKNCNVFVYYANDTAILQQGVMANGDFFDERHGLDWLQNYVQTNL
                     YNLLYTSTTKIPQTDAGVTRLLSNVEQSMDQSVTNGLVAAGVWNGGPIGQLNSGDTLT
                     KGYYVYAQPLSEQAQADREARKAPLIQVACKLAGAVHYADVQINVVR"
     gene            complement(10953..11510)
                     /locus_tag="OO349_003227"
     CDS             complement(10953..11510)
                     /locus_tag="OO349_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5868198.1"
                     /translation="MGELRLSNNTSTERGWLIPTSGDPDYDEALDRLLSQWMRNVSGL
                     SAGMVRPRWQKEQPPLLPVETNWCAFGVIGWSGDDSPAFTRQTDDGSQLWRHETIECM
                     ASFYGPAGMVYASRFRDGISVPQNNAALNALGLSLGDYTGLFPELINQQWVRRYDMTV
                     RLRRKVVREYGIKSLVEAPVIFFGD"
     gene            complement(11485..11874)
                     /locus_tag="OO349_003228"
     CDS             complement(11485..11874)
                     /locus_tag="OO349_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001687820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail adaptor"
                     /protein_id="HCR5868199.1"
                     /translation="MPTLDVTDVLFDPDFCDFNLWVTRRVQTVDEDGIGSDSEVKKQF
                     AGVVTVDRSLENRRMQAGQVISGAILIVTTKRLTQGQTGRDADIVTYQGRDYRVTFVD
                     PYTAYGAGFVQAHCELMPFDGGTPVEQ"
     gene            complement(11861..12415)
                     /locus_tag="OO349_003229"
     CDS             complement(11861..12415)
                     /locus_tag="OO349_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5868200.1"
                     /translation="MSFKSGVTTRVDNAQAILDALRSLTKKDVLVGIPEEDSEREDVP
                     FGNAGIGYVNEYGSPAQNIPPRPHLIPGVKSVEEQTVPQLKAAAQAALDGNAAGAERA
                     LNRAGTLAANGVRRYMTITGFTPLADSTVEARARRGRKGATLELARRVAGESPGTDLA
                     KPLIDTGQYRRAITHVVRDKDADS"
     gene            complement(12412..>12806)
                     /locus_tag="OO349_003230"
     CDS             complement(12412..>12806)
                     /locus_tag="OO349_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4054 domain-containing protein"
                     /protein_id="HCR5868201.1"
                     /translation="SLPAVSDFRRDFPQFADPAKYPEAQIQFRLNLADELLSENVTGK
                     DLFPYFAGLFVAHYMTLWAADSRAMLVGGPGGSTNGVQSSKSVDKVSVSYDTSATLNP
                     DAGFWNNTRYGAEFYQLITMFGAGGRQL"
BASE COUNT         2958 a         3546 c         3194 g         3108 t
ORIGIN      
        1 gattccgaga tttccactgc atcatcagga atattggtgc cgtgaaattc agtcgaataa
       61 aatccgtttg tcgatgcaga ataaaaatat gacatttcgt taatatccca ttgcaatgta
      121 atacgcatca gcagatgaac catatcctga accgggcgac actgtaatat caaatccagt
      181 gttatggaca tttgtcgccg atacagtcgg ctgccctgac gcgccagtaa cgttcaatgt
      241 aactgtgaca gcaaggactc ttgagaatgt catcgggaac ctcatcccgt atgttgaaga
      301 tacattcggg gagccattca actgaccgca ctgtaaaatt aatccagacg gaaatttttg
      361 cgcagcagcc gttgcggtat tacttgacgc gaacaggctc atatcaggta tctgattcgc
      421 ccctgtacct acattccttt tagccgcttc tcccaaacca aggtatgtga gaatgtcagc
      481 aatagtattt ttcccaataa tgtcacggcc aacagaagtt aaatcagtct gcgctgctgt
      541 atcatttcca gtgaaatatg ggagtttatt tgcacctgtt gcgagcccag ctaatgccga
      601 cagcgtggca tcaagcgcct ggaaatcttt cccgaaagcg gtccccattt tggatataaa
      661 cccgttcagg tctccatcat caagcacatc cagcctgctt ttgttggcgg tgtactgcgc
      721 caacgctgcc gcgataaagc tggcttgccg aatagctttg ttgacctgtg cgctggatgc
      781 tttacctgcc gtaaaccctg aaagcagagc cggaagtgct tcccagtcag cttgtgaggt
      841 gacgttagcg ttcggatcaa gcgcgaaagg tttaaagttg tttattgcca ttagagtatt
      901 gtcccccatg ctccaacatc gaacccgccg atgtattcgt tatccatatc aaacccaaag
      961 aatttagagc cctcggatgg tgcttctacc gaaggcgttt caacatcacc ggcccatacg
     1021 ccagctgctt taacggtgag atagccctgt ttgatagcgg caatcagttc aagagacaca
     1081 tcagaaatat cagtctcggg gaatacccaa accgatatcg tcatgtcctg gttgtcgaca
     1141 atttgcatcc tcaggcctga gccttcagtc gcagcgtcaa ggatgggagg cagagagtcg
     1201 ttccgaccgt cccagttgtt gatagcgatt ttcgctttca gaatgatgcg gtacgtctca
     1261 tcgcttagcg tcgtatagcc agaatcagga tcatatggcc ctttccagat gccctggtca
     1321 tacccaagcc cgtcagtgtc ccagctgaaa taaactccgc taattggctg gctgactatg
     1381 cgactgcgtc cgatccacag accgaggatg tcgagctgta caccgacagc agtatcaata
     1441 tcgaaggctg ttataagccc tgacatagtg ctggacacat caatcagcgg gcgggtgctc
     1501 agatctatat ggtcaaaaaa gagtggcttg gtagcgtggt agttagtgat cagttcggtg
     1561 tatttgctca tgaggtcacc gtgatactga tattcgcggt gctacaggac gcagaagcat
     1621 cataggcaat atcaatgttt gatgccgata cgctgccaga cgacttaccg atcagcaggt
     1681 cggtaatatc gtaatagcgg gcattcccgc cgctcacaac gccgaggttt gccggggaat
     1741 aaatacggct cagcagaacg tcgtcgccaa ttgttaggcc atttatataa tcggcaacag
     1801 cctgtttaat ctgctcgccg atttgagagg tatagccggt aaaaactttc agggtaatgg
     1861 ctacgaaaat tggcacatcg gtagagcgcg aaaaactgat gacgtgtgga ttaccgtaag
     1921 tatccggcac cgtgacagaa gttttaccgt aagttgcggt tccctgccct ttactccccc
     1981 ggattgtttg ggctatctcg gtaacatccc ctccatcgac gatggcggaa atagagtgtg
     2041 gcggcagccc gttgctgtcg gttgccccag tgtcgttctc atatagtttg tgacgtgtca
     2101 cgccagcaac attagcgata gcaccgtcga caccttcaaa cggtgtgatc gatggtagcg
     2161 cgacgctttg cccctgccga atgcgcagct ctgcgtcggt ttcggctggt gaaccgacag
     2221 tagccgcaac tggattggtt accgacaccc aacctcgggt cggggtgttg atagtggtaa
     2281 tagtcccggc cagcgccgca accgaaccgc tattcgcaca tgtggccgtc accagcacag
     2341 taccatcaac gccgatcgct acacttgcgg gaaaattcca gataatgccg tttttatccc
     2401 gtgcggagcc attcgtgata gtcgtgcctg ccgtaccggt taacagaagg tcagcagtag
     2461 agtttgtcgc tacttttcgc gtgatcccgt taattttcac attgctgcta agcgctgcgg
     2521 cctgcgctgt cgtcggtgaa aaagagttgt agatcccgat agcggtgttg ttagcgtcat
     2581 gcacggcaag agccaccagc gcgaccatct gcccgtcttt gctgtctggt tcgaggtagg
     2641 catcactacc gtaaatctgc ctgaaatagc tggtcagtgt atctaggatt gtctggtaat
     2701 caggcgcact aatcccctgg gcggttaccg ttgccgatag ccccagcgtg tcgaggttca
     2761 aagccattta tgcctcgctt gttacagtcg tctggccgta gattgtgtca atggaggaag
     2821 tgaaggtgac gcgacggctg gtgccgtcat aattggtatc gaaggaaaga atcgacagaa
     2881 cgcccggtgt atcctgtatg cgttcgcgta tagccaggat gtagacatct gatcgctgct
     2941 tcccaagcac tgactgaaca tacggcgtgc cttccgtcag atcgagaaac cactgaccgc
     3001 gccacagctc gaaacgggtt tttacggcct gggcgacaca ttccggactg tcgataagga
     3061 aagtatcgtc accctggccg aaagtgtaat cgccttcagt atcttcgcga cggtatcgca
     3121 ttattgcggc cctccggtag ttcccccgcc agtctgtact ccgccatgtt tatgcgtggc
     3181 gacactgata ccagaagctg ttacgtcatt cgttaccatc accggcccaa gcatcgtcgc
     3241 agtaccgccg ctttcgccca ttccctgaga caagttgcca ttaatcgtta cgttgccgtt
     3301 cagcgtgata gtcggggatg tgattgtcgt tccaccttca gccgtagctg taagctggcc
     3361 cggtgtttta atggtgatgt tatgtcctgc ggcgacttct acaaacgccg caccatcatc
     3421 ggttcgcagc tgcgcggcgc tggtactgat accgctgatt ttccgtgctt gcgactgcgg
     3481 gccaacgatg gcgaacgcat cagataagtc atgctggcgc gggtcgacgg tctcctgaac
     3541 gccgccgctc tgccaccaaa aatcgatgca acggtcagca aaaatcagga ggcactcgtc
     3601 gccttctttt accggaaagg tcagcgtgca accgccgccg cgcgggaaga tgaccggcac
     3661 atccaccagc ggttttaatt cggtggatcc atcgccaaca ataccgcgta gcgccacctc
     3721 tactgtgcag gtaacagtct caggatcgaa cgactgaatg atgccgggca tcgctacgcg
     3781 catctgggta gacaccgaat cggcaatggc ctgcgcggtc tgctgctcac cgccgatctg
     3841 tgattgagtt ggaattggca taaaaccccc ataaaaaaac cagccgaagc tggtttggta
     3901 tcaattatct cttagtaact aagctacatc agtgtgaagt aaggcttttc cgctgcaatg
     3961 gcacacattc acaaggtgta ttaggatatg tttgcaacaa gtaagcattg aaaatcaatt
     4021 tcagccatga agccgccata gagtggacct gcaccatcgc attattgact acaggagtaa
     4081 caaatgggat ttagatttcg taaaagaatc cggattgcgc ccggactcgc gatcaacatt
     4141 agtaaaagcg gagtaagcac ttcaattggt ggtaaaggtt ccaccattaa catcgggaaa
     4201 aaaggcgtaa agatgacaaa tggccttccc ggcacggggc tgtcgcatac cactaacctt
     4261 tactctcccg ggaaatcgac agaaaaaaaa caacttactc ataagcaaaa aataattaga
     4321 aacatcctgt tcgtaattat tgtgtttata ataattaaag ctcaatattt ttgacgcatg
     4381 cccgcctatc tggcgggcta ttttactttt cggcagtcgt atgttgcata ctgacgcggc
     4441 gaatccatgc tggcttgcag ccactgtgcg ttgagaatgg ctttgccgtt tcgattgatg
     4501 tactcaagac caacccatct tccaggctga tcggtagcca tacgccaatc catcttgata
     4561 ttgttataat cgcctttgtt tttcaggaag gtgatttttt gcatttctgg ctttgcgcca
     4621 ttgattctgg ctaagccatc atctgcccaa tggattttaa aatcaccaca ttgctgatcc
     4681 gcaaaagcgg gtgctgatat aagcaccgcg aacacggtaa cagtgcaaag taaatgtctc
     4741 acggccccac ctcactaatt cgtcgtccct tgcattgctt tcgggctata aagatcacga
     4801 gcgccacgcg caaaacacat caaatccatg taccacgcct gacctctggt gtcgccagta
     4861 tagtcgatag ctttgacgat ataaacgcca tccgtcgcaa tgctggcagc ctgtgacgtc
     4921 gtgccggtca gcacacggtt gccgttctct tctgtttcgg tgatacgccc gggcgactgt
     4981 gctatttcgc tattgccgag cgtggcgcgg tacaccgaag cctgatcgag ctggataaga
     5041 ccattaatac ggatgtttgg gtttatcagg catcgcacgt ttactccgcc acccatcgtt
     5101 tgttgcggca taccgatcag gccagtattg gcattcaaca caatggcttc gtgaatatat
     5161 ttatcctccg gcaccatctg gacctgacca tccaccagtt gccatgtcgc tttgcactgc
     5221 gcagcaatat tatccatcac gttgcggctg gatgagtaaa tcgcgcggcc acgaggaaac
     5281 acggtatcag gaaaatcgcc ggtaatgccc tgtgtcacgc cgaacgcgtt gaaatcctgc
     5341 atcgtcgccc ggtgcagatc cgcaacggta tatccagcgg caagcgtggt gatggtggtc
     5401 gcatagagga acgcttcgtg atcaccaatg gcctgaatca acacccagga atccgtaatg
     5461 ttgtccttcc cggtgacggt gaagcgaata tcaccgtcaa aaatcaggcc gtagttctga
     5521 ccgttcacct gccctatctg gtctggtgaa atctcccggg acacaccaac ctggctcgca
     5581 tcaacatccg gcgctatacc gtcatacccg gcaatgatgc gaattttggc aaactcctgc
     5641 ccaagtatct tgttcgtggt atcggtcgaa aggttgtaaa ttttcacgtt cgccacgcgc
     5701 ggccagcgtg tgtctgccca ctcgatctgg aacgtgacct taaagtcaga cagggaaacg
     5761 ccctgcccgt tctggtccaa cagttgcagc tcaaaatggc gcatccagtt aagagacatt
     5821 tctactcctg tacgaaaatg aggtggctgt atgtgccgag gttggttttg gtgggctcgt
     5881 ctggtgcgcc cttatcggtc gccaccacca gcgcgccatc aatgccaagc tgtggatatt
     5941 gtcgtaaaag gtttacgccg gtcagtagag gtacgccaga gagaagcgcg gcaccgccgc
     6001 tatccatcac gtccataatc cagccagccg catcacgcca gatgatcctg agtgtatacg
     6061 tggtattgcc cagcaaaact cggaactgct gattgtcagg agaaagcggt atttcgttaa
     6121 actgcatatc atcccccgaa tgctgatgta acgcttccgc ccagctggct cagcaaggat
     6181 tcgtttggcg gtgtagtgga tttcatcccg gaattctgca ccgccgatgt gctgacgcca
     6241 tcctgcatat ctgatttatc tgcaacgcta acgctctgcg tctgcgacat gatcacttca
     6301 cgcagggtaa gcgtgcagtt cagcacgttc tcgctggttt tatccgttgt cacctcgatg
     6361 gctcgcacca gcatattgct gtaaacccgc tttccggtca ctacatcgaa cggcacccta
     6421 gaggactgga gatccagtag ttgctgatag gtctctttcg ggctaagtcc ggcgctgagg
     6481 ccgattgacg atgtatcaat gaagtccagc aacgaaccgc cacctgcgaa gccgcattcc
     6541 atcgtgactt cgctggggcg cttatacgca tgatcggcga tgaaacccga cgcgctattc
     6601 gttgttggct tctccaccgg gtgctcagta atttcgagcg catcagaatg cttttcagaa
     6661 acaaccacgc tggggattaa tattccaatt cgccgggatt gctggcgaaa aattgctgat
     6721 aaaatatcca ttatctcggt cctgcgggga gttgctgggt taactgtgaa ttcacgccct
     6781 tttgacggtc aacagtcaaa cgggcagcct cgcgcggatc gaaaacgccg tggatgttaa
     6841 tattcgtttc ctgctgaatc accggggcgc tggtgggcat attgctcatt actttcggaa
     6901 tgtagttgcg cgtttcctgc ggcattagcc ccattccata acgcttaaca ttcccgatcc
     6961 cccagttata tgatgccagt gctttgctaa ggtctccgcc gttctgccgc aacagctggc
     7021 tcaggtactt agcggctgcc tgagctgact tttccgggtc gaatacatca ttcccacgca
     7081 ggcccatgtc gcgcgccgtg ccatccataa actgaaacag acctttagcg ccagcgcctg
     7141 aaacggcgaa ctgattaccg cccgattccg ttatggccac gctgcgcaat aacccctccg
     7201 gaagccggta gagctgttcc aggttggtaa acatcggctg catccatccc agcagttcag
     7261 agcctgcttt ggttggctgt ggccgcttaa ctgactggcc gagctgctca ggctcatcat
     7321 caccgaacca gccgcgcacc gttcggccca cgctgcgagg atcgaatccc cagtgctctt
     7381 taatccagtc ggcggttccg ttggcgctgt ctgttaccat cggcatcgct gacggatttt
     7441 cgctgccctg attaagcatc tgtttgccga tgctggcggc atcagcccag cggccatctt
     7501 taatggcgtt gagcaggtcg gcgatcatgt tcagcatctt gccgaactca cccatttgcg
     7561 aaatgaagtt gctaaaatcc cactttaggg accatgactt ggggtcaata ccgagcaact
     7621 tagccagcgc ttttgtcagg tccgtaacag tctttttcag gtcaccaatc attttgacgg
     7681 cctgatcaat ttcagtcttc catttccccc agtcaatcag gcttttgcca ccttctttcc
     7741 acgtctggta atcatccaag agcagaccaa gtgcaataat aagcgtcgtt atcatgccca
     7801 agggtgatga catgaaagcg gtatttagca gccgccatgc aaccaacagc ccactgaaca
     7861 atgcgataag ctgctgcgtg gctgggttca gctttttgaa ccaggcgatc acaccctcaa
     7921 cagcttgccc agttcgccat aacacgcggg taatcgcgtc acctgcccag agaattcctt
     7981 tgatgatctt cgtgatgacc gcttcaatct tcggccagtt gtcgagtatc tgcctgcgga
     8041 agttatcaat actgccagca aggccaccag ctaggttaga gccaatcttg tctttcgcct
     8101 gtccaagcgt catcgtcaga ttacgcatgg aggtcatgaa aatattggac tgtttagccg
     8161 ctgactcagc attaaaacca atacgctttg ccgtcaacgc gtactcagaa ctgagctgcc
     8221 ccatccctct gcgcatcgcc atcagcgtgt tttcatcgat gccgagcatc tgcgcgtatt
     8281 gcttcgcgcg gtaatacggc atgttgttga gcttttggcc aacgccagta aagatggcag
     8341 cagtatcacg catctttccg ctggcatcgc gggtctggac accaagacgg ttcaggaacc
     8401 cttccgcccc cggattgcta cgcatgaagc cagccaaccc ttcgagggag gacatagccg
     8461 actcggcgct ggcaccggtt tgcgatgcgg cgtatcccag cgctttgatg ccctggacac
     8521 tggcccccgt ccgctgggat gcccagtaaa ttttatccag accgttcgcg atctgagtgg
     8581 taaatccgac aatgctcagc gctgcgcctt tcaccaccgc gccgaccttc agaacgttcg
     8641 cggtaacgcc tttcagcacg gcttcaaact tattagcgcc agcctgatcg atatcgaatc
     8701 ccagcgaaac aaggaaatct ttaatcgtat ctgcgttacc gctcattggc cgctctccat
     8761 ttatctaccc gggcgtcgtt atcctcgcgc atgtcgaggt agtcattgag aagcgcgatg
     8821 cggcaaagat ctaccgcacc gctgttaagg tctttctggt caatatggaa ggcaagcgcc
     8881 gggcgaagaa taaaatcttc accgcccggc aggctgttga aggttattcc gctagcgggg
     8941 tgggcgtctc gctggtaggg agtccttgca aaaaatttcc cagcgagtcg gcgaccaccc
     9001 gcgccaccag ttgcagcatg gtaagcaggt cgatatcgtc aaacgccatt tcgccatgct
     9061 ggcagaccgg cacccagcct ttcatgtgct cgcgtgaaac aacggaaaga caggggaaca
     9121 ggatagcgtc cacgtcgcca tcactcagat cggacacagc attggcaatc tttggcagga
     9181 tagtagccat cgcgccttcg gtgtctttgc tgctgatctt ctcctgaacg ctccggaagt
     9241 ccgaaaccat cccggccaga accggcaaca gcttgcggga caccttcagc tgttcgaaaa
     9301 cgctgagctt tgcggtgcga tatttcacgc ctttaatttc gaattccatg cgttaaaact
     9361 ccccgagaag ctggtcaatc ttgccgcagt cgaataccca ggcgacggtt ccgccctctt
     9421 tagcgttatt gaaatcaggc tgtttctgga atgcacacga acgcgcagta gaaatatcac
     9481 ccgatgccgt gttgcgaatg acgatcacgt tattgcccca ggtggcagag gactggcttt
     9541 gcgcgttata cgccagagac agctttttat tcaccgggga ggttttgagt agcgtcaccg
     9601 taatggtgcc tgacttatcg gcgtgcaggc tgtgcatcac ctcgccatcg gcaccgatag
     9661 tcatggtgtt tttgttgccg cccatggtct gggtgatacc ttcctcagag ttcgcagaac
     9721 cctgaccaag atcgataacg ccggtcggcc cggtgagcga cgcggttaca tcgagaaaag
     9781 aataagttgc catttatcgc tccttagcga accacgttga tctgcacatc ggcataatga
     9841 actgcgccag ccagcttaca ggccacctgg attaacggtg ctttgcgcgc ttcacggtcg
     9901 gcctgcgcct gttcggacag cggttgcgca tacacgtaat agcctttggt cagcgtatcg
     9961 ccggaattca gctgtccgat agggccacca ttccacacgc cagcagctac cagaccgttc
    10021 gtgacggact gatccatgga ctgttcaacg ttggaaagca gacgggtcac accggcatca
    10081 gtctgcggaa ttttggtggt gctggtgtaa agcaggttat agaggttggt ctgaacgtag
    10141 ttctgcaacc agtcgagccc gtggcgctcg tcgaagaagt caccgttcgc catgacaccc
    10201 tgttgcagga tcgccgtgtc gttggcgtag tacacgaata cattgcagtt ttttgcatca
    10261 agtgccgatg cctggctgac tgtcagtgtt tcatacccga cacccggctc ctgcttaaac
    10321 ttgagcgtaa tcgcggtatt actgccattg aaattaaccg tgaatgcccg gccaaatgca
    10381 gataacgcag cgtatttatt acccgatgaa tattgaataa aactgcgtga atatccggcg
    10441 gttttcagtt ttgatgccaa atcatcgctg gatgcagtct gcaggcattt ctcatcgctt
    10501 gtcgtaatcg ccagaatacg gcttacagaa gaggattcga tcgccgcagc cactttcagc
    10561 cagtctgcat ccggaatatc ttcatcgtct gcaatcccca gcccatacca tgaagtataa
    10621 tcgagcatgg cattcacagc ctgctccagc gtctcaggcg tggcctgttc gctgtctccc
    10681 ttcgttttca cccaacgacc aacaaaaacc tcctgaggtt tcggtgattg tgagaaaaac
    10741 acctgcgcag ccttatattc tggtgattcc acgccaaaat cttttccaat atcttccgcg
    10801 gcagaataac ggcgaatgcg ctcacttacc ggaatgattg tggacgggcc gagaatgagt
    10861 aatgcaccaa aatttcgccc tgatgctgca cgcggcgaca tgatcacatc aacattaaca
    10921 acgtttgata caggcaagcc ctgtgccata gcttaatctc cgaaaaagat gactggtgct
    10981 tccaccagcg atttaatacc gtactcgcgc acaaccttcc ggcgcagacg caccgtcata
    11041 tcgtagcggc ggacccattg ctgattaata agttcaggga agagacctgt gtaatcgcca
    11101 agagacagcc ccagcgcatt cagtgctgcg ttgttctgcg gtacagatat accgtcacga
    11161 aaccgggacg catacaccat ccccgccgga ccataaaacg aagccataca ctcaatcgtt
    11221 tcatgccgcc agagctgaga gccatcatcg gtctgtctgg tgaatgccgg actgtcatca
    11281 cctgaccatc cgataacccc aaacgcacac cagttcgttt caaccggtag cagtggcggc
    11341 tgctctttct gccagcgcgg gcgaaccatc ccggcagaca gaccggaaac gttacgcatc
    11401 cactggctta acagcctgtc gagcgcttcg tcataatccg gatcgccact ggttggtatt
    11461 aaccatccgc gctctgtact ggtgttattg ctcaaccgga gttcccccat caaacggcat
    11521 caactcacaa tgcgcctgaa cgaatccggc cccataagct gtatacgggt cgacgaaggt
    11581 cacacgataa tcacggccct gatacgtcac gatatcggca tcacggccag tctgtccctg
    11641 cgtcagtcgc ttagtcgtca caatcagaat tgcaccgctg attacctgcc ctgcctgcat
    11701 acggcggttt tccagagagc gatcaacagt tacgactccg gcaaactgct ttttaacttc
    11761 actgtcgctg ccgatcccgt cctcatccac cgtttgcact cggcgtgtta cccacaaatt
    11821 gaagtcgcaa aaatcggggt caaaaagcac atctgttaca tcaagagtcg gcatctttat
    11881 ccctcacaac atgggtaata gctctgcgat attgcccggt gtcaattaat ggtttcgcca
    11941 gatcggttcc gggagattcg ccagcaacac gccgagcaag ttccagtgtt gcccccttgc
    12001 gcccccgacg agcccgggct tcaacggtgc tatcagcaag cggtgtaaag ccggtaatgg
    12061 tcatgtaacg cctgacgcca ttcgcggcca gcgttccggc gcggttaagc gctctttccg
    12121 cacccgccgc atttccatca agcgcagcct gcgccgctgc tttgagctgc ggcaccgtct
    12181 gttcctctac ggatttaacg ccggggatca ggtgcgggcg tgggggtatg ttttgcgctg
    12241 gtgagccgta ttcgttgacg taaccgatcc cggcattacc aaacggaaca tcctcacgct
    12301 cgctgtcttc ttccgggatg cccaccagca catccttttt ggttagcgac cggagcgcat
    12361 ccagaatggc ctgagcgtta tcaaccctcg ttgttacacc acttttgaaa ctcatagctg
    12421 gcgaccgccc gcaccgaaca tcgtgatcag ctgataaaat tcagcgccat atcgggtgtt
    12481 attccagaag cctgcgtcag ggtttagcgt cgcgctggtg tcatagctga cgcttacctt
    12541 gtcaacggac ttggaggact gaacaccatt ggttgaaccg cccgggccgc caaccagcat
    12601 tgcccgacta tctgccgccc atagcgtcat gtagtgagcc acgaacaacc cggcaaagta
    12661 cggaaacaaa tctttgccgg tgacgttttc gctcagcagt tcatcggcca gattcagacg
    12721 aaactggatt tgcgcttcgg gatatttggc agggtcagca aactgcggga agtcgcggcg
    12781 aaaatcactt accgctggca ggcttt
//
LOCUS       DAJTQY010000103        12695 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.103, whole genome shotgun sequence.
ACCESSION   DAJTQY010000103 DAJTQY010000000
VERSION     DAJTQY010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12695
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.103"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(100..738)
                     /gene="rutR"
                     /locus_tag="OO349_003231"
     CDS             complement(100..738)
                     /gene="rutR"
                     /locus_tag="OO349_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HCR5868202.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQT
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            1026..2117
                     /gene="rutA"
                     /locus_tag="OO349_003232"
     CDS             1026..2117
                     /gene="rutA"
                     /locus_tag="OO349_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HCR5868203.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIAQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSIPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQATEQTGRDVGSYVLFMVIADETDDATRAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLSALTQEVA"
     gene            2117..2809
                     /gene="rutB"
                     /locus_tag="OO349_003233"
     CDS             2117..2809
                     /gene="rutB"
                     /locus_tag="OO349_003233"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HCR5868204.1"
                     /translation="MTTLTARPEAITFDPQQSAQIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEHFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            2821..3207
                     /gene="rutC"
                     /locus_tag="OO349_003234"
     CDS             2821..3207
                     /gene="rutC"
                     /locus_tag="OO349_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HCR5868205.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFYIQCGLVKPDALVEIATIAHIAK"
     gene            3215..4015
                     /gene="rutD"
                     /locus_tag="OO349_003235"
     CDS             3215..4015
                     /gene="rutD"
                     /locus_tag="OO349_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HCR5868206.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIERYAVVGHALGALVGMQLALDYP
                     ASVTMLVSVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPTACSS
                     ELHAALPDSQKMVMPYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            4025..4615
                     /locus_tag="OO349_003236"
     CDS             4025..4615
                     /locus_tag="OO349_003236"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HCR5868207.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLQELYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            4626..5120
                     /gene="rutF"
                     /locus_tag="OO349_003237"
     CDS             4626..5120
                     /gene="rutF"
                     /locus_tag="OO349_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization flavin reductase protein
                     F"
                     /protein_id="HCR5868208.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            5141..6469
                     /gene="rutG"
                     /locus_tag="OO349_003238"
     CDS             5141..6469
                     /gene="rutG"
                     /locus_tag="OO349_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HCR5868209.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSYAAWFGLPHFSTPGFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNANLIMVAVTF
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(6551..6658)
                     /locus_tag="OO349_003239"
     CDS             complement(6551..6658)
                     /locus_tag="OO349_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5868210.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGKSSHGKSDN"
     gene            7031..7627
                     /gene="wrbA"
                     /locus_tag="OO349_003240"
     CDS             7031..7627
                     /gene="wrbA"
                     /locus_tag="OO349_003240"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCR5868211.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            7648..7875
                     /locus_tag="OO349_003241"
     CDS             7648..7875
                     /locus_tag="OO349_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HCR5868212.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(7913..9154)
                     /gene="agp"
                     /locus_tag="OO349_003242"
     CDS             complement(7913..9154)
                     /gene="agp"
                     /locus_tag="OO349_003242"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HCR5868213.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGIVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPMITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRASAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(9447..10703)
                     /locus_tag="OO349_003243"
     CDS             complement(9447..10703)
                     /locus_tag="OO349_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccE family protein"
                     /protein_id="HCR5868214.1"
                     /translation="MGSNIHGISCTANNYLKQAWNDIKNEYEKNQTYSITLFENTLVC
                     FMRLYNELRRKVNEEDTPCLECESLEKEFEEMQNDNDLSLFMRTLRTNDTQIYSGVSG
                     GITYTIQYVQDVDIVRVSLPGRGSEPITDFKGYYWYGFMEYIENINACDDVFSEYCLD
                     DENMSIQPEQINMPGISDLDTGIDLSGISFIQSEINKTYGLKYAPVDGDGYCLLRAIL
                     VLKEHEYSWALGSHKTQKQVYEEFIKIVDKQTIEALVDTAFYNLREDVKTLFGVDLQS
                     DNKIQGVGSFMSWSFLFFKKQFIDSCLNDKKCILHLPEFIFNDNKNLLALDTDTSDRI
                     KAVKNFLAVLSDSICSLFIVNSNVASISLGNESFSTDEDLEYGYLTNTGNHYDVYLPP
                     ELFSQAYKLKNKEMNAQLDYLNRYAT"
     gene            10964..11884
                     /gene="cbpA"
                     /locus_tag="OO349_003244"
     CDS             10964..11884
                     /gene="cbpA"
                     /locus_tag="OO349_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HCR5868215.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGTKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            11884..12189
                     /gene="cbpM"
                     /locus_tag="OO349_003245"
     CDS             11884..12189
                     /gene="cbpM"
                     /locus_tag="OO349_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HCR5868216.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(12282..>12695)
                     /gene="torD"
                     /locus_tag="OO349_003246"
     CDS             complement(12282..>12695)
                     /gene="torD"
                     /locus_tag="OO349_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HCR5868217.1"
                     /translation="TLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQEIKR
                     LLVEAGMETSGNFNEPADHLAIYLELLSYLHFSLGEGTVPARRIDSLRQKTLTALRQW
                     LPEFAARCRQYDSFGFYAALSQLLLVLVECDHQNR"
BASE COUNT         3033 a         3244 c         3198 g         3220 t
ORIGIN      
        1 tcgctgagtg tgaataccgc tgccgctggc ggtaacgcca gtttaatgac gatcggttaa
       61 acaggtagcc ggaggatgtt acaacctcct ccgtcatctt taacgtggtc gaatcccctc
      121 aataataatc cgctgcacgt tttcaaccgt ttgattgaaa aatacctcat cgcgcaacgt
      181 cgcgcctgtc accgcctcca cctgaggggc gaaatcggcg taatgttgag tggaagccca
      241 aatcataaaa atcaaatgct gcggatcaat cggcgcgagt ttgccgcttt tgacccaacc
      301 ggcaatcagc gccgatttct catcaattaa tgccttcaaa tcgcccgtca gttcatccat
      361 taacaacggc gcgcctgcca gcatctccat acagaacagg cgcgaagcct gcggataatc
      421 gcgtgagact tccagcttca gacggatgta ctcttttatc gccgccagcg gggcgaaatc
      481 ttcacgaaac gcttttaacg gtgccagcca gatatcgaga atctgccgca gcacggcaat
      541 atacagcgcc tcttttgacg ggaagtaata cagcagattg gtttttgaaa cacccgccaa
      601 ctctgcggtc tgctccagtc ttgtgccgtg aaaaccgaat tgtgaaaaag tgtccagtgc
      661 tgcgctaaga atcgcttttt tcttcgcgct tactgcgcgc gaacgtttac ccgttgtttt
      721 cactgcgcct tgcgtcatgc gctctcccct cttgtagatt ggtaacagga tagcaaaacc
      781 cccgcccggc ctcgaccttc tgcactctca tcgcgcacgg atgcatgttt tatgtgcaac
      841 tgttttgacc gtttagtcca ctttttacca gatattttag ataacacaat gctaacacat
      901 taataacatt cactttttaa aaactggcat ccgctttgca aacaagccaa tacgcggctg
      961 aaaaggatat gcaggatgca gcgccccgtc tgactttcac tttacgagac gaagagaggt
     1021 tgatgatgaa aattggcgta ttcgtaccta ttggcaacaa cggctggctc atttcgaccc
     1081 acgcgccgca gtacatgccg acctttgaac tgaataaagc cattgcgcaa aaagcggagc
     1141 attaccattt cgatttcgcc ctgtcgatga tcaaactgcg tggctttggc ggcaaaactg
     1201 agttctggga tcacaacctt gagtcgttca ccttgatggc gggtctggca gccgtgacct
     1261 cgcgcattca gatttacgcc actgctgcca ccttaacgtt acctccggca atcgtcgccc
     1321 gtatggccgc aaccatcgac tccatctctg gcgggcgttt tggcgtcaac ctcgtgactg
     1381 gctggcaaaa gcccgagtat gagcagatgg gtatctggcc tggcgatgac tatttctccc
     1441 gtcgttacga ctatctcacc gaatatgttc aggtgctgcg cgacctgtgg ggcacgggga
     1501 aaagcgattt taaaggcgat tttttcacca tgaatgattg tcgcgtcagt ccgcaaccga
     1561 gtatccccat gaaagtgatc tgcgccgggc aaagcgacgc tggcatggcg ttctccgccc
     1621 agtatgccga tttcaacttc tgtttcggca aaggcgtaaa tacacccacg gctttcgccc
     1681 cgaccgctgc gcggatgaaa caggccacag agcaaaccgg acgcgacgtt ggctcttatg
     1741 tgttgtttat ggtgattgcc gatgaaaccg acgatgccac tcgcgccaaa tgggaacact
     1801 acaaagcggg cgcggatgaa gaggcgttaa gctggctaac cgaacaaagt cagaaagata
     1861 cccgctccgg tactgacacc aacgttcgtc agatggccga tcccacttcg gcggtaaaca
     1921 tcaatatggg gacgttagtc ggttcttacg ccagtgtcgc gcgcatgtta gatgaagtcg
     1981 caagcgtgcc tggtgcagaa ggcgtgctgt tgaccttcga tgattttctg tcgggaatcg
     2041 aaaccttcgg cgagcgcatt caaccactga tgcagtgccg cgcccatctc tctgcgctga
     2101 ctcaggaggt ggcatgatga caaccttaac cgctcgaccg gaagccatta ccttcgatcc
     2161 gcagcaaagt gcgcagatcg tggtggatat gcaaaacgct tatgccacgc caggcggcta
     2221 cttagatctc gccgggtttg atgtctcaac cactcgcccg gtcattgcca acattcaaac
     2281 cgctgtgacc gcagcgcgag cggcagggat gttgatcatc tggtttcaaa atggctggga
     2341 tgaacagtat gtcgaggctg gcggccccgg ctcaccgaat tttcataaat cgaacgccct
     2401 gaaaaccatg cgtaagcagc cgcagctgca ggggaaattg ctggcgaaag gctcctggga
     2461 ttatcaactg gtggatgaac tggtgccgca gcctggcgat attgtgctgc cgaagccgcg
     2521 ctacagcggt ttcttcaata cgccgctgga cagcatttta cgcagccgtg gaatacgcca
     2581 tctggttttc accggtatcg ctaccaacgt ctgcgtcgaa tcgacgctac gcgatggctt
     2641 ttttctggag catttcggcg tggtgctgga agacgcaaca caccaggcgg ggccggaatt
     2701 tgcacagaaa gccgcgttgt tcaatatcga aacctttttt ggctgggtca gcgacgtcga
     2761 aacgttctgc gacgcgcttt ctcccacgtc ctttgctcgt atcgcttaag gagtttagcg
     2821 atgccaaaat ccgtaattat tcccgctggc agcagcgcac cgctggcccc cttcgttccc
     2881 ggcacgctgg ctgatggcgt ggtgtatgtc tccggtacgc tggcttttga tcaacataat
     2941 aacgtgctgt ttgccgatga cccaaaggcg caaacccgcc acgttctgga aactatccgc
     3001 aaggtgatcg agacggcggg tggcacgatg gcggatgtga ccttcaacag catctttatt
     3061 accgactgga aaaattacgc cgcgattaac gaaatctacg ccgagttttt tccgggtgat
     3121 aaaccggcgc gattctacat tcagtgcgga ctggtaaaac ctgacgcgct ggtggaaatc
     3181 gccacaattg cgcatatcgc caagtgaggc cgcgatgaaa ctttcactct cacctccccc
     3241 ttatgctgat gcacccgtag tggtgttgat ttcgggtctt ggcggtagcg gcagttactg
     3301 gttaccgcaa ctggcggtgc tggagcagga gtatcaggta gtctgttacg accagcgcgg
     3361 caccggcaat aatcccgaca cactggcaga agattacagt atcgcccaga tggcagcgga
     3421 actgcatcag gcgctggtag ccgcagggat tgagcgttac gcggtggtcg gccatgcgct
     3481 cggtgcgctg gtggggatgc agctggcgct ggattatccc gcgtcggtaa ctatgctggt
     3541 cagcgttaac ggctggctac gaataaacgc ccatacgcgc cgctgttttc aggttcgcga
     3601 acgattactg tatagcggcg gcgcgcaagc atgggtggaa gcgcagccgt tgttcctcta
     3661 tcccgccgac tggatggcgg cccgcgcacc tcgcctggag gcagaagacg cgctggcact
     3721 ggcgcatttt cagggcaaaa ataatttact acgtcgactt aacgccctca aacgcgctga
     3781 ctttagtcac catgcggatc gcatccgctg cccggtgcaa atcatctgcg ccagtgatga
     3841 tctgctggtg ccaacagcat gttccagtga acttcatgcc gccctgcccg atagccagaa
     3901 aatggtgatg ccctatggcg gacacgcctg caacgtgacc gatcccgaaa cgtttaatgc
     3961 tctgttactc aacgggcttg ccagcctgtt acatcaccgt gaagccgccc tgtaaggaat
     4021 tgctatgaac gaagccgtta gcccaggtgc gcttagcacc ctgttcaccg atgcccgcac
     4081 tcacaacggc tggcgggaga cacccgtcag cgatgagacg ttacaggagc tttatgccct
     4141 gatgaaatgg gggccgacat cagctaactg ttctccggca cggatcgtgt ttatccgcac
     4201 tgcagaagga aaagaacgtc tgcgcccggc gctttccagc ggcaatctgc aaaaaaccct
     4261 gaccgcgccc gtcaccgcta tcgtcgcctg ggacagtgaa ttttatgaac ggttaccact
     4321 actgtttccc cacggtgatg cccgcagttg gtttacctcc agcccacaac ttgccgaaga
     4381 aacagcgttt cgcaacagtt ccatgcaggc ggcttatctg atcgtcgctt gccgtgcgct
     4441 gggactggat accggcccga tgtcgggttt tgaccgtcaa cacgttgacg acgccttttt
     4501 tgcgggcagc acgctgaaga gcaatctgct gattaatatc ggctatggcg atagcagcaa
     4561 actttttgcg cgcctgccac gtctgtcctt tgaagaagcc tgcgggctgt tgtaaggagc
     4621 aaactatgaa catagttgat caacaaacct ttcgcgatgc gatgtcctgt atgggcgcgg
     4681 cggtcaatat catcaccacg gacggtccag ccgggcgcgc cgggttcacc gccagcgccg
     4741 tctgcagcgt gaccgacacg ccgcccacat tactggtgtg cctgaatcgt ggggcgtccg
     4801 tctggccggt attcaatgaa aatcgaacgc tgtgtgtaaa tacactgagc gccgggcagg
     4861 agccgctttc aaatcttttt ggcggcaaaa cgcccatgga acaccgcttt gccgccgccc
     4921 gctggcagac gggtgtgacc ggatgcccgc aactggaaga ggctctggtt tcgtttgact
     4981 gccgtatcag ccaggtggtg agcgtcggca cccacgacat tctgttttgc gccatcgaag
     5041 cgattcatcg tcacaccaca ccctacgggc tggtgtggtt cgatcgcagt tatcacgcgc
     5101 tgatgcgccc tgcttgttaa cctccttaag gagacagctg atggcaatgt tcggttttcc
     5161 tcactggcag ttaaaatcga cctctacaga gagcggcgtg gtcgcgccgg atgaacgatt
     5221 gccgtttgct cagacggcag taatgggcgt tcaacacgcg gtggcgatgt ttggcgcgac
     5281 ggtgttaatg ccgattctga tggggctgga tcccaatctt tccattttaa tgtcggggat
     5341 cggcacgtta ctgtttttct ttattaccgg cgggcgcgtc cccagttatc tcggctccag
     5401 cgcggctttt gtcggcgtgg tgattgccgc gacgggcttt aacggtcagg gcattaaccc
     5461 gaatatcagc atcgcgctgg gcgggattat cgcctgtggg ctggtttata ccgtcattgg
     5521 cctggtggtg atgaaaattg gtacacgctg gattgaacga ctaatgccgc cggtggtgac
     5581 gggtgcggtg gtgatggcga ttggcctgaa cttagcgcct atcgcagtga aaagtgtatc
     5641 ggcttcggcc tttgatagct ggatggcggt aatgacggtg ctgtgtattg ggctggtggc
     5701 ggtgtttacc cgtggcatga tccagcggtt attgattctc gtaggattaa ttgtcgcctg
     5761 tctgctgtat ggcgtgatga ccaacgtttt agggctggga aaagccgtcg attttactct
     5821 cgtcagctat gctgcctggt tcggtctgcc gcatttttcc actcctggtt ttaatggcca
     5881 agcgatgatg ttgattgcgc ccgtggcggt gattctggtg gcggaaaact taggtcatct
     5941 caaggcggtc gccgggatga ccggacgcaa tatggacccg tatatggggc gagcattcgt
     6001 cggtgatggt ctggcaacga tgctttccgg ctctgtcggc ggtagcggcg tcaccaccta
     6061 tgctgaaaat atcggtgtga tggccgtgac aaaagtctac tcgacgctgg tgtttgttgc
     6121 agctgccgtc atcgctatgc tgttgggctt ttcgccgaaa tttggcgcgc tgatccatac
     6181 cattccggcc gcggtgattg gtggtgcatc aattgttgtg ttcgggctga ttgccgtcgc
     6241 aggcgcaagg atatgggtac aaaaccgtgt cgatttaagc cagaacgcta atttgattat
     6301 ggtcgcagta accttcgtgc tgggcgcggg tgattttgct ctcacgcttg gcggttttac
     6361 gttgggaggg atcggtacag caacctttgg cgcgatttta ctcaatgcgt tattaagcag
     6421 aaggttggtt gacgttccac cgccagaagt tgttcatcag gagccataaa cagaaaaccg
     6481 ccgggttacc ggcggtgatg actttttgac gagcttaaga aaaaaatgca ttcgtcattg
     6541 attagcccgg ctagttgtcg cttttgccgt gactgctctt gccacctttt ttacctgctt
     6601 ctgatgctct ttcgcggtct tctgcaaaat tgccggaacc gcctcgatgg tttgccatta
     6661 ctgacctccg tctgggtata gacatcatat tgcatagaat taacgcggcg tatggcggct
     6721 gccatatttt cgctacgtgg gactaagctt agtgaactga cgcggacaga cccgcgtttg
     6781 gtaatatcct gcaacagctt cttagcgaga atagatatca tctccaataa ttatccataa
     6841 gccgctctta tgattaaaaa aacgcgatga gataaaaatg tgtcattttg cgacaaaatt
     6901 acgtgcttgt gaaagttgtt ataaatcaaa taagtggttg tgaaatttgc actctgaaaa
     6961 ggacgtctta tctttaaata agtggtagcg aatcgctacg gaatagagat aacacgagga
     7021 gtggttagaa atggctaaag ttctggtgct ttattattcc atgtacggac atattgaaac
     7081 gatggcacgc gcagtcgctg agggtgcaag caaagtggat ggcgcagaag ttgtcgttaa
     7141 gcgtgtaccg gaaaccatgc cgccgcaatt atttgaaaaa gcaggcggta aaacgcaaac
     7201 tgcaccggtt gcaaccccgc aagaactggc cgattacgac gccattattt ttggtacacc
     7261 tacccgcttt ggcaacatgt ccggtcaaat gcgtaccttc ctcgaccaga cgggcggcct
     7321 gtgggcttcc ggcgcactat acggaaaact ggcgagcgtc tttagttcca ccggtactgg
     7381 cggcggtcag gaacaaacca tcacatccac ctggacgacc cttgcgcatc acggcatggt
     7441 gattgtcccc attggctacg cagcgcagga attatttgac gtttcacagg ttcgcggcgg
     7501 tacgccgtac ggcgcaacca ccatcgcagg cggtgacggc tcacgccagc caagccagga
     7561 agaactgtct attgctcgtt atcaagggga atatgtcgca ggtctggcag ttaaacttaa
     7621 cggctaatct tcaacaggag gatacgcatg ccaactcaag aagcgaaagc tcatcacgtc
     7681 ggtgaatggg catctctgcg caatacgtcg ccggaaatag ccgaggccat tttcgaagtc
     7741 gccgggtacg acgaaaaaat ggcagaaaag atttgggaag aaggtagtga tgaagtgtta
     7801 gtcaaagcct ttgctaagac cgataaagat tcgctttttt ggggcgaaca gaccatcgaa
     7861 cgtaaaaacg tttaaccagc gactcccccg cttctcgcgg gggagttttc tgttatttca
     7921 ccgcctcatt caacacgcta tcaaacttat ccatcgggca gaaaccgttg gcatctatcg
     7981 ggcaaccgct taattccagc gtcacgcgct gcgcaggtgc ctgcagggtt aacgcatcgg
     8041 cattacgtaa ctgttccgca ctctggtaca catattcaat tttcatcaaa tcgcgattgg
     8101 ctttgctgtc atgccaacgc tggaaaacga ttttgccgcc aatcggcgtg cgttcgttct
     8161 ggtcatgcaa ctgatacggt ttgaaatcca gcgccgttaa cagagaggct atgttggagt
     8221 cgtgcccaac taacaccgtg attttcggag cactggcgcg atcggtgacc agggctttgt
     8281 cgatataact gaccagcggt ttcgcaacat tgcgcgccac ttccggtgag gtaaacagac
     8341 tgtcctggta accgtttttc agcttcgaca ataccttcca ctgctggtca gatttgattt
     8401 ctccccaggc cacctgatcc atcggaaaac cttcgtagta ttgcaaagta aacgcatcta
     8461 ccagcgagtt gccgactttc agcggcccgg aaacacctgg ttcttgttga tacttggcgc
     8521 taaaggtatt tttgccatcc accagcgaac actgttgttt ctctttacag gcaggggaat
     8581 ctttatagtt aacgattttt tccagtagct ggtagctgtc ggtaagctgg agtttgctga
     8641 gctctttctc cattgccgcc accgcctgtt cactgaatgc ggcggaatca tcggtgatca
     8701 tcgggttaaa ggttgggtcc atagtgccca ttttttcctg gtgatgcaca ggaatatcac
     8761 acccagggaa tgcaccagta ataaagaact gggcggtagc gacggtacgt tgcagactat
     8821 tggcataggc ataaacggtg tccggcggcg ggcattcccc cgatttcact atcccctgct
     8881 ctgccagcca ttcacgcatg taatggccca tatacacttc gagcacgcca cctttggtgg
     8941 tgagttgccc accggggacg tcccattcgg gccatttatt cggcgtcgac tgctccagca
     9001 cactgccatt gttcgccagc ggcgcacgta agttatggcg gctcatcatg agcacttgct
     9061 gtagctgata gccttccggt acggtttgtg cctgagcatt tgaagcgagt aaaactatcc
     9121 ctgccacagt tgcggcgatt agcgttttgt tcattcctgg cacctctttt gttatcaatc
     9181 attaaagagt gtgacagaaa tatgactttt ttccgggaac aggttcgcaa aatggcttgt
     9241 tgaacgcctg tttttctata tacatcttca aaaacaggca aggtttgacg caaactatgc
     9301 ctgattagcc cctgcctcgc gtacgccgct gataattgca cctgtggcta aacagaatat
     9361 ctgcggcgtt accaatacag agacaatatt taccaataaa aaagtgtgag actaataaca
     9421 atggttttta gcatatgcct ttgccattaa gttgcataac gatttaaata gtcaagttgc
     9481 gcattcattt ccttattttt taacttataa gcctgagaaa aaagttcagg agggaggtaa
     9541 acatcataat gattgccagt gtttgttaaa taaccatact caagatcttc atctgttgaa
     9601 aaggattcat ttcccaagga gattgatgcc acattactat taacaataaa taatgagcaa
     9661 atgctatctg aaagaacagc aagaaaattc ttcaccgctt taatcctatc cgacgtgtcg
     9721 gtatctaaag caagcaaatt cttgttatca ttaaatataa actcgggtaa atgcagtatg
     9781 cattttttgt cattcaagca actatcaatg aattgttttt taaaaaacag aaagctccat
     9841 gacataaaac tacccacacc ctgaattttg ttgtcagatt gcagatcaac gccaaataat
     9901 gtctttacat cttccctgag attatagaat gccgtatcaa caagagcctc gatcgtttgt
     9961 ttatcaacaa ttttaataaa ctcttcgtat acttgctttt gcgtcttatg gctgcccagc
    10021 gcccatgaat actcatgttc ttttaaaacc agtatggctc ttaacagaca atatccatcg
    10081 ccatctactg gagcgtattt taatccatat gttttattaa tttcactctg aataaaagat
    10141 atgccagaca aatcaatacc agtatctaaa tcagatattc ccggcatatt tatctgttct
    10201 ggctggatac tcatattttc atcatctaaa caatactcag aaaaaacatc atcacacgca
    10261 ttaatgtttt caatatattc cataaagcca taccaataat aacctttaaa atctgtgata
    10321 ggctctgaac ctctgcccgg caaggacact ctaacaatat caacatcttg aacatattgt
    10381 atagtatatg taatacctcc tgaaacccct gaataaattt gtgtatcatt agtacgcaat
    10441 gttctcataa ataatgatag atcattatca ttctgcattt cctcaaattc tttttctagt
    10501 gattcacatt ccagacatgg agtatcctct tcatttactt tacgtctgag ttcattgtac
    10561 aaccgcataa aacacaccag tgtgttttca aaaagcgtga ttgaatatgt ttgatttttt
    10621 tcgtactcat tttttatatc gttccaggcc tgtttcaaat aattatttgc agtgcaacta
    10681 attccatgta tattactacc catatatagc gtctataaaa tttaataaat aatgacgccc
    10741 tagttaaact taaagtgcct ggttcaacta tcaaaaatcg ctcacccttt ttcacctgtt
    10801 taaaatatgt tcagcaaccc atcttgatgg cgacctcctc tccgcgatga tttcaataac
    10861 atattctgtg ttggcatatg aaattttgag gattacccta cacttatagg agttacctta
    10921 caggggttcc ttcaatttgt gttgatttac gcgagataac gctatggaat taaaggatta
    10981 ttacgccatc atgggcgtga aaccgacgga cgatctcaag acaatcaaga ccgcctatcg
    11041 tcgactggcc cgtaaatacc atcctgatgt cagcaaagaa ccggatgccg aagcccgctt
    11101 caaagaggtc gctgaagcct gggaagtgtt aagtgatgaa caacgtcgcg ctgagtatga
    11161 tcagatgtgg caacatcgca acgatccgca atttaaccgt cagttccatc atggcgacgg
    11221 tcagagtttt aatgccgaag attttgacga tatcttctcg tcaattttcg gtcagcatgc
    11281 ccgccagagc cgtcaacgcc ccgccgcacg cggccacgat attgaaatcg aagtggcggt
    11341 attcctcgaa gaaacgctta ctgagcataa gcgtaccatc agctataacc tgccggttta
    11401 taacgccttt ggcatgatcg aacaggaaat cccgaaaacg ctgaatgtga agatcccggc
    11461 aggcgtcggc aatggtcaac gcatccgcct gaaaggccag gggacgccgg gcgaaaacgg
    11521 cggtccaaat ggcgatttgt ggctggtgat tcatattgcg ccacatccgc tgttcgatat
    11581 tgtcggccag gatctggaaa ttgtggtgcc ggttagcccg tgggaagcgg cgctgggtac
    11641 taaagtcacc gttccaacac tgaaagaaag cattttgctg actatcccgc caggcagcca
    11701 ggccgggcaa cgattgcgcg ttaaaggcaa aggtctggtg agcaaaaaac agaccggcga
    11761 tctgtatgcg gtactgaaaa tcgtgatgcc gccgaaaccg gatgaaaaca ctgccgcgct
    11821 gtggcagcaa ctggcagacg cccagtcgtc ttttgatcca cgtaaagatt gggggaaagc
    11881 ataatggcta atgttacggt gacttttact attaccgaat tttgcctgca taccggcatc
    11941 tctgaagagg agttgaatga aattgtcggt ttgggggtgg ttgaaccgcg tgagatccag
    12001 gaaacaacct gggtatttga cgaccatgcc gccattgtgg tgcaacgcgc ggtacgcctg
    12061 cgtcatgaac tggctctgga ctggccgggg atcgcggtgg cgctgacgtt aatggatgat
    12121 attgcgcacc tgaagcagga aaaccgcctg ctacgccagc ggctttcccg gtttgtagct
    12181 catccgtgat gtgtgtttgc cggatgtgat gacgcatccg gcaactgcgc cataatgcct
    12241 gatgcgacgc taaacgcgtc ttttcaggcg cataaacgac gttatctgtt ttggtggtcg
    12301 cactccacta acaccagcaa taactggctt agtgccgcgt aaaaaccaaa gctgtcatac
    12361 tgtcgacaac gcgcagcaaa ctctggtaac cattgccgca gcgccgtcag tgttttttgc
    12421 cgcaaactgt cgattcttcg cgcaggaacg gtcccctctc ccagcgaaaa atgcagataa
    12481 ctcaacaact cgagatagat cgccagatga tctgccggtt cgttgaaatt gccactggtt
    12541 tccatccctg cctcaactaa caagcgttta atctcttgct cgtcctgttt gtaggccgat
    12601 gcatacggca gcgccgcttg tttgtcagtc atcagaaaca ggccgcaaaa gtcagcggcc
    12661 agttccagac gggcatcgtc acgtactgtc agcgt
//
LOCUS       DAJTQY010000104        12414 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.104, whole genome shotgun sequence.
ACCESSION   DAJTQY010000104 DAJTQY010000000
VERSION     DAJTQY010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12414
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.104"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            <1..1199
                     /gene="edd"
                     /locus_tag="OO349_003247"
     CDS             <1..1199
                     /gene="edd"
                     /locus_tag="OO349_003247"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HCR5868218.1"
                     /translation="ALLESEAASYHAPGTCTFYGTANTNQMVVEFMGMQLPGSSFVHP
                     DSPLRDALTAAAARQVTRMTGNGNEWMPIGKMIDEKVVVNGIVALLATGGSTNHTMHL
                     VAMARAAGIQINWDDFSDLSDVVPLMARLYPNGPADINHFQAAGGVPVLVRELLKAGL
                     LHEDVNTVAGFGLSRYTLEPWLNNGELDWREGAEKSLDSNVIASFEQPFSHHGGTKVL
                     SGNLGRAVMKTSAVPVENQVIEAPAVVFESQHDVMPAFEAGLLDRDCVVVVRHQGPKA
                     NGMPELHKLMPPLGVLLDRCFKIALVTDGRLSGASGKVPSAIHVTPEAYDGGLLAKVR
                     DGDIIRVNGQTGELTLLVDEAELAAREPHIPDLSASRVGTGRELFSALREKLSGAEQG
                     ATCITF"
     gene            1236..1877
                     /gene="kdgA"
                     /locus_tag="OO349_003248"
                     /gene_synonym="eda"
     CDS             1236..1877
                     /gene="kdgA"
                     /locus_tag="OO349_003248"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HCR5868219.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(1933..3111)
                     /gene="purT"
                     /locus_tag="OO349_003249"
     CDS             complement(1933..3111)
                     /gene="purT"
                     /locus_tag="OO349_003249"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HCR5868220.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSKSLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVVLATSESVVDAIERAKHAAGQVKVQG
                     "
     gene            3245..3535
                     /gene="yebG"
                     /locus_tag="OO349_003250"
                     /pseudo
     CDS             3245..3535
                     /gene="yebG"
                     /locus_tag="OO349_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
     gene            3602..3958
                     /locus_tag="OO349_003251"
     CDS             3602..3958
                     /locus_tag="OO349_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HCR5868221.1"
                     /translation="MKKRGAFLGRLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            4285..4944
                     /locus_tag="OO349_003252"
     CDS             4285..4944
                     /locus_tag="OO349_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HCR5868222.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVSGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDANERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            5154..7214
                     /gene="ptrB"
                     /locus_tag="OO349_003253"
     CDS             5154..7214
                     /gene="ptrB"
                     /locus_tag="OO349_003253"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HCR5868223.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPKVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDTLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(7211..7873)
                     /gene="exoX"
                     /locus_tag="OO349_003254"
     CDS             complement(7211..7873)
                     /gene="exoX"
                     /locus_tag="OO349_003254"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HCR5868224.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSLLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGLKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERYPGYLRWLFNNLNSMSPELRLTLKHYLE
                     NT"
     gene            complement(7897..8553)
                     /locus_tag="OO349_003255"
                     /pseudo
     CDS             complement(7897..8553)
                     /locus_tag="OO349_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
     gene            complement(8655..8885)
                     /gene="holE"
                     /locus_tag="OO349_003256"
     CDS             complement(8655..8885)
                     /gene="holE"
                     /locus_tag="OO349_003256"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HCR5868225.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            9024..9398
                     /gene="yobA"
                     /locus_tag="OO349_003257"
     CDS             9024..9398
                     /gene="yobA"
                     /locus_tag="OO349_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HCR5868226.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            9402..10274
                     /gene="copD"
                     /locus_tag="OO349_003258"
                     /pseudo
     CDS             9402..10274
                     /gene="copD"
                     /locus_tag="OO349_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
     gene            10287..10628
                     /locus_tag="OO349_003259"
     CDS             10287..10628
                     /locus_tag="OO349_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HCR5868227.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(11021..12097)
                     /locus_tag="OO349_003260"
                     /pseudo
     CDS             complement(11021..12097)
                     /locus_tag="OO349_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
     gene            complement(12063..12344)
                     /locus_tag="OO349_003261"
     CDS             complement(12063..12344)
                     /locus_tag="OO349_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HCR5868228.1"
                     /translation="MNLVTLKTWGKLRYPDNPPSISTLRRWARNGNIYPAPELHGRSY
                     RVVPEAFYINPNKVDTDITHHQPNGRQGRDSPLMEKLKHAAEKIRSQFA"
BASE COUNT         3079 a         3032 c         3137 g         3166 t
ORIGIN      
        1 tggccttact ggagtcagaa gccgcgtctt accatgcgcc gggaacatgt actttctacg
       61 gtactgccaa caccaaccag atggtggtgg aatttatggg gatgcagttg ccaggctctt
      121 cttttgttca tccggattct ccgctgcgcg atgctttgac cgctgccgct gcgcgtcagg
      181 ttacacgcat gaccggtaat ggtaatgaat ggatgccgat cggtaagatg atcgatgaga
      241 aagtggtggt gaacggtatc gttgcactgc tggcgaccgg tggttccact aaccacacca
      301 tgcacctggt ggcgatggcg cgcgcggccg gtattcagat taactgggat gacttctctg
      361 acctttctga tgttgtaccg ctgatggcac gtctctaccc gaacggtccg gccgatatta
      421 accacttcca ggcggcaggt ggcgtaccgg ttctggtgcg tgaactgctc aaagcaggcc
      481 tgctgcatga agatgtcaat acggtggcag gctttggtct gtctcgttat acccttgaac
      541 catggctgaa taatggtgaa ctggactggc gggaaggggc ggaaaaatca ctcgacagca
      601 atgtgatcgc ttccttcgaa caacctttct ctcatcatgg tgggacaaaa gtgttaagcg
      661 gtaacctggg ccgtgcggtt atgaaaacct ctgccgtgcc ggttgagaac caggtgattg
      721 aagcgccagc ggttgttttt gaaagccagc atgacgttat gccggccttt gaagcgggtt
      781 tgctggaccg cgattgtgtc gttgttgtcc gtcatcaggg gccaaaagcg aacggaatgc
      841 cagaattaca taaactcatg ccgccacttg gtgtattatt ggaccggtgt ttcaaaattg
      901 cgttagttac cgatggacga ctctccggcg cttcaggtaa agtgccgtca gctatccacg
      961 taacaccaga agcctacgat ggcgggctgc tggcaaaagt gcgcgacggg gacatcattc
     1021 gtgtgaatgg acagacaggc gaactgacac tgctggtaga cgaagcggaa ctggctgctc
     1081 gcgaaccgca cattcctgac ctgagcgcgt cacgcgtggg aacaggacgt gaattattca
     1141 gcgccttgcg tgaaaaactg tccggtgccg aacagggcgc aacctgtatc actttttaag
     1201 acgacaaatt tgtaatcagg cgagagaaaa ctctgatgaa aaactggaaa acaagtgcag
     1261 aatcaatcct gaccaccggc ccggttgtac cggttatcgt ggtaaaaaaa ctggaacacg
     1321 cggtgccgat ggcaaaagcg ttggttgctg gtggggtgcg cgttctggaa gtgactctgc
     1381 gtaccgagtg tgcagttgac gctatccgtg ctatcgccaa agaagtgcct gaagcgattg
     1441 tgggtgccgg tacggtgctg aatccacagc agctggcaga agtcactgaa gcgggtgcac
     1501 agttcgcaat tagcccgggt ctgaccgagc cgctgctgaa agctgctacc gaagggacta
     1561 ttcctctgat tccggggatc agcactgttt ccgaactgat gctgggtatg gactacggtt
     1621 tgaaagagtt caaattcttc ccggctgaag ctaacggcgg cgtgaaagcc ctgcaggcga
     1681 tcgcgggtcc gttctcccag gtccgtttct gcccgacggg tggtatttct ccggctaact
     1741 accgtgacta cctggcactg aaaagcgtgc tgtgcatcgg tggttcctgg ctggttccgg
     1801 cagatgcgct ggaagcgggc gattacgacc gcattactaa gctggcgcgt gaagctgtag
     1861 aaggcgctaa gctgtaaaag tcaaaatgcc cgatctagga tcgggcattt ttgtagcgtt
     1921 tttttgccgg gtttaaccct gtacttttac ctgtccggcg gcgtgcttcg cgcgttcaat
     1981 ggcgtcaaca acactctctg aagtagccag taccaccccc agacgacggc tgccatcaat
     2041 ttccggctta ccaaataaac gaatctgcaa atctgcgcct acggcattct gcacattatc
     2101 aaacgtgaca ttctgactgg tcagttgtgg cagaataacg gcagaagctg caggaccata
     2161 ctgacggatc ccgccaacag gaagtccgag gaaggcacgt acatgcaggg caaactctga
     2221 gagatcttga gaaattaacg tcaccatccc ggtatcatgt ggacgagggg agacctcact
     2281 gaaaatcacc tcatcaccac agacaaacag ctcgacacca aacagaccat aaccgcccag
     2341 tgccagcacc actttacggg caatctcctg cgcacgttca agggcaagcg ggctcatttg
     2401 ctgtggttgc caggattcac ggtagtcgcc atcttcctgg cgatgaccta ctggtgcaca
     2461 gaaatggacg ccatccaccg cgctgacggt tagcagggta atttcgaagt caaacttaac
     2521 gacgccttca acaattacgc ggcccgctcc ggcgcgaccg ccttgctgag cgtattccca
     2581 tgcctgagca agttgctctg cagagcgaat aaacgtctgc cccttgccgg aagagctcat
     2641 caccggtttt acaatgcagg gatagccaat ggcggcaacc gcctcgcgga aaaggctttt
     2701 gctatcggca aaacgataag tggaagtggg cagctgcagc tcttctgccg ccaggcgacg
     2761 gataccctcg cgattcatcg ttaatttcgt tgcgcgagcg caggggacaa cattcagtcc
     2821 ttcctcttca agttggatca gcatatcggt ggcaatagct tcgatctccg gcacgatata
     2881 atgtggtttt tccagttcaa ccacacggcg taatgcatca ccatcaagca tattaatgac
     2941 atgggagcga tgcgcgacat gcatggctgg tgcgtcggca tagcgatcga cggcaatcac
     3001 ctctacgccg agacgctgac actcgattgc cacttcttta cccagttcac cggagcctaa
     3061 taacatcacg cgagttgctg ccggacgcag cgcagtgcct aataacgtca taacgaatat
     3121 cccctgatta attccgctcg cagtataaac gaaaacgttt gcgtgtgtct ttattgctga
     3181 tgttgatttc tcaaccgaaa agaaatatac tgtataaaat ctcagttatt atgagaggct
     3241 gcaaatggcg gttgaagttt aatacgtagt cattcgtgag ggcgaagaga aaatgtcgtt
     3301 taccagcaaa aaggaagccg atgcttatga caaaatgctt gatacggcgg atctgcttga
     3361 cacctagctg acaaattctc cagtgcaaat ggaagacgag caacgtgaag ccctttcgct
     3421 atggctggca gaacaaaaag aggtgctgag caccattctg aaaaccggca aattaccgtc
     3481 cccacaggtc gtcggtgctg aatccgaaga ggaagatgcc tcacacgcgg cctgaagcaa
     3541 cttgcgctcc tgacaatttg taccatgctt tctcctgaat cattgtgcta tggagaaaaa
     3601 catgaaaaaa agaggggcgt ttttagggcg gttgttggtt tctgcctgcg catcagtttt
     3661 cgctgccaat aatgaaacca gcaagtcggt cactttccca aagtgtgaag gtctggatgc
     3721 tgccggaatt gccgcgagcg taaaacgtga ttatcaacaa aatcgcgtgg cgcgctgggc
     3781 agatgatcaa aaaattgtcg gtcaggccga tcccgtggcc tgggtcagtt tgcaggacat
     3841 tcagggtaaa gatgataaat ggtcagtacc gctaaccgtg cgtggtaaaa gtgccgatat
     3901 tcattaccag gtcagcgtgg actgcaaagc gggaatggcg gaatatcagc ggcgttaatg
     3961 gcttctgata attcgattga ccgttggcgg atgcggcgcg agtgccttgt ccgtcccaga
     4021 acgcaccaca atcgtaggcc tgagaagacg caacagcgtc gcatcgggca atcagcatca
     4081 aacaatgcca ctcaaaacga caaactactt tcacctaaaa tacccttatc acttgatata
     4141 cagtaaatac cttacctggc gcgaatttct ttgaataacg gcctaccgcc ccgcttattt
     4201 gcgcatcttt tgtctcgata tacattttct gacatcctca tccgctatgc tacaggcact
     4261 aaaaatggtt attgaggagc aaatatggct aactggttaa atcaactgca atccctgctt
     4321 gggcaaagca gttcttctac ctcctcgtct gcggatcagg gattgggcaa actgttagtg
     4381 tcaggcgcat taggcggact ggctgggctg ctggtcgcaa ataaatcagc acgtaaactt
     4441 cttacgaaat atggcaccaa cgcgttactg gttggcggcg gagcggtagc gggtacggtg
     4501 ctgtggaata aatacaaaga taaaattcgc gcggcgcatc aggacgaacc gcagtttggc
     4561 gcgcaaagta cgccgctgga tgagcgtacg gaacgtttga tccttgcgct ggtctttgcc
     4621 gctaaaagtg atggtcatat tgatgccaat gaacgtgcgg caatcgacca gcaattgcgt
     4681 gaagccggcg tggaagagca ggggcgtgta ctcattgagc aggcaatcga acaaccgctg
     4741 gatccacaac gcctggctac cggggtccgc aatgaagaag aggcgctgga aatctatttc
     4801 ctgagctgcg cggctattga tattgaccat tttatggaac gcagttacct gaatgcactg
     4861 ggtgacgcgc tgaaaatccc ccaggatgtt cgtgaaggca ttgaacgcga tctcgaacag
     4921 caaaaacgca ctttggcgga ataattccgc tctctggata gcctgaagtt gactatttcg
     4981 ggctatccag aaccgcacag ccatttgaac aggctgattt tctctgtctg cataacattc
     5041 gatatccgcg ccattacctg tcatcatcta agcaatgacg accctgtttc gcttgcatcc
     5101 ccggtgagtt ttgccaccct tataagatgt ttccaaccag aaagaacaat aacatgctac
     5161 caaaagccgc ccgcattccc cacgccatga cgcttcatgg cgatacgcgc atcgataatt
     5221 actactggct gcgggacgat acgcgttctc agccaaaagt cctggactac ctgcaacaag
     5281 aaaatagtta cggtcatcgg gtgatggcct cacaacaagc cttgcaggat cgcatcttaa
     5341 aggaaatcat cgaccgcatt ccgcaaagag aagtttctgc gccctacata aaaaatggct
     5401 accgctatcg gcatatttat gaaccaggct gtgaatatgc tatctaccag cgtcaatcgg
     5461 cattcagcga agagtgggat gagtgggaaa cattgctcga tgccaacaag cgcgcagctc
     5521 atagtgagtt ttattcgatg ggcggaatgg cgattacgcc cgataacacc attatggcgc
     5581 tggcagaaga ttttctttcc cgacgccagt acggcattcg ttttcgtaat ctggaaacag
     5641 gtaactggta cccggaactg ctggataacg ttgaacccag ctttgtctgg gcaaatgact
     5701 cctggacttt ctactatgtt cgcaagcatc cggtgacgct gctgccttat caggtctggc
     5761 gtcacgccat cggtacgcca gcatcgcaag atacactgat ctacgaagaa aaagacgata
     5821 cctattatgt cagcctgcat aaaacgacgt cgaagcacta tgtcgtcatt catctggcca
     5881 gcgccaccac cagtgaagtt cgcctgctgg acgcggaaat ggccgatgcc gagccgtttg
     5941 tttttctgcc gcgccgcaaa gatcacgaat acagccttga tcactaccag catcgttttt
     6001 atctgcgttc caatcgccac ggcaaaaact ttggcttata ccgtacccgt atgcgtgatg
     6061 agcaacagtg ggaagagtta attccgccac gcgaaaacat catgctggaa gggtttacgc
     6121 tgtttaccga ctggctggtg gttgaagagc gtcagcgcgg gttaaccagt ttgcgccaaa
     6181 ttaaccgcaa gacccgggaa gtcattggca ttgcctttga tgatccggcc tatgtgacct
     6241 ggattgccta caatccagaa cctgaaaccg cgcgattgcg ttatggttat tcttccatga
     6301 ccacaccaga cactttgttt gaactggata tggataccgg tgagcgtcgt gtattaaaac
     6361 aaacagaagt gcctggtttt gatgcggcga attaccgcag tgaacacctg tggatagtcg
     6421 cccgtgatgg cgtcgaagtt ccggtttcgc tggtctatca tcgcaaacat tttcgcaaag
     6481 gacacaaccc gttgctggtg tatggctatg gttcttacgg cgcaagtatt gatgccgatt
     6541 tcagttttag ccgcttgagt ttgttagatc gtggctttgt ctacgccatt gtccatgttc
     6601 gcggcggtgg tgagctgggg caacaatggt acgaagacgg taaatttctg aagaagaaaa
     6661 atacgtttaa tgattatctt gatgcctgcg atgcattgtt aaaactgggc tatggctctc
     6721 cttcgctctg ttatgcgatg ggcggaagtg cggggggcat gttgatgggc gttgcaatta
     6781 atcaacgccc ggaattattc cacggcgtta tcgcccaggt accgtttgtt gatgttgtaa
     6841 caacgatgct tgatgaatca attcctctta ccactggtga gtttgaagag tggggaaatc
     6901 cgcaggatcc gcaatattac gagtacatga aaagctacag cccgtatgac aacgtcaccg
     6961 cacaggctta tccgcattta ctggtaacga ccggtttaca cgattctcag gtgcaatatt
     7021 gggaaccggc aaaatgggtc gctaaattgc gcgagctgaa aaccgatgac catcttttat
     7081 tgctctgtac cgacatggac tcaggccatg gcggtaaatc tggccgcttt aaatcgtacg
     7141 aaggcgtagc gatggaatat gcttttctgg tcgcgctggc gcagggaaca ttacccgcta
     7201 cgcctgcgga ttaagtattt tccagataat gtttcagtgt taaacgcagc tccgggctca
     7261 tgctgttcag gttattaaat aaccagcgca gatagcccgg atagcgttcg gcaacgtcgg
     7321 aaaccgcttt gccacggtat ttgccaaagg tgaaggtcgt catcaacgac ggacgtccgg
     7381 tgatatcggc catctgttct gccgtccagc cggaggtgtt cataatatcg ataagcaacg
     7441 cggcggtgat ataacagtca tacaacgcgc ggtgatgatg caggcccggc ggtgtctgta
     7501 cattgagctt gcgtgtttta tataacgcca tattgctgta cttgagccct ggccacaaac
     7561 gacgggccag tttcatagtg caaatccact caccgggcat ctcaggcagt acgcggcggt
     7621 caaagctggc gttgtgcgcg acataccatt cactaccgta atagtgtggg atcacatctt
     7681 caatccacgg tttatcggcg accatggctt cggtgatgcg atgaatcgcc atcgcttgtg
     7741 gactaatagg acgatcgggg cgcaccagga ggctcatggg gttgacgatt tttccgtcaa
     7801 tgacatcaac agaggcaatc tcaacgatcc ctccctgcaa accgcaggtt tctgtatcga
     7861 taatgcgcaa catggcatgc tccaggccga aaaagcctag cgtaatggaa tgatatcgcc
     7921 ttgccaaccc tgtgatgaac gctgaccgac taacaataat gaaccacgat cggcgcgaac
     7981 gatgagacga ccatattcat cccatagtgc actgttacca cgggcatttg ccatcagcac
     8041 ggcaatcgag tactgatgtg aaaaaaattg taagcgacgg gccgacgcca gcaggtcggg
     8101 ttctcccaga cattgccctg tcgtaaacag tgaacaggtc gggtccatat ccataccttg
     8161 cggttgttca tcgaccaccg tgatggttct ggaacgacgg cccagacatg caccatgact
     8221 ctgatggtag ttcccaggcg tttttcgcca cggtgcgaac accgcaattc cacgaataaa
     8281 gcgatcgtta tattcaacgg gaaggccggc aatgatggtc attcgctagg tcgttgcggc
     8341 ataacaaagc gggtctaaca gtgaaagatc gggtggggca ggaagggcac gtcgcgaata
     8401 atcacacccc agtaaagaaa gcgatggaaa aactaacagc tgacattgtt gcctggcggc
     8461 ggccctcaca aactccagat gatgagctac ctgttcggtg agtgaggttt tacgaggctc
     8521 atattgcgct gctgcaactt tccagaacga cataatgact tccttttcaa aatgactgca
     8581 aattatgggg gcggtttaga aaatactcat aatttagcag gcgttatgta agaaagtgta
     8641 actctgtata agttttattt aagtttgggc tcgtaaggta aacgtgacag attgaccgaa
     8701 gccaaacggt gggcaataag ccgctcgcga aaccagctgc gcaaatgttc tggctgttca
     8761 cgttcaaccg cttcagcgat caccggcata ttgtagcgtt ctttaaaagc caccccggcc
     8821 gccgccaaat cgacattcac tttatccatt tctgtttgat ccagtttagc cagattcttc
     8881 agcattctta atctcctcat cattcgcggg caaaagttac taagagtgag cctggatggc
     8941 aagccggaat tgatgatcct cgactcagac attagacaag gttatgctct gtaaaaaagt
     9001 tattacaaaa aggaatagat aatatggctt caactgcacg ctcccttcgc tacgcgctgg
     9061 caatcctgac cacttcgttg gtgacacctt ccgtctgggc acatgcgcat ttaacgcatc
     9121 agtatcctgc ggcaaacgcg caagtgacag ctgccccgca ggcaatcact ttaaacttct
     9181 cggaaggcgt tgaaacagga ttcagtggtg caaaaatcac ggggccaaaa aacgaaaata
     9241 ttaaaacatt accagcgaag cggaatgagc aggaccaaaa gcaactgatt gttccactgg
     9301 ccgattcgct gaaacccgga acctacaccg ttgactggca tgttgtttcg gtggacgggc
     9361 ataaaacgaa aggacactac acctttagcg tgaaataaat catgctggcg tttacctgga
     9421 tcgcgctgcg atttatccat ttcacctcgt tgatgctggt ctttggcttt gcaatgtatg
     9481 gtgcctggct ggccccctta atgattcgtc gcttgctgac gaagcgttct ctgcgtttac
     9541 aacagcacgc tgccgtctgg agtttgatca gtgccactgc aatgctcgcc gttcagggcg
     9601 ggttgatggg aacgggatgg acagatgtat tttcgccaaa catttggcag gctgttttgc
     9661 aaacgcagtt tggtggcgtc tggttatggc aaattgttct cgccctcgta acgttgatcg
     9721 tcgcccttat gcaaccgcgg aatatgccac gtttgctgtt tatgctcact accgcgcaat
     9781 ttattctgct ggcgggagtg gggcatgcga cgctgaatga aggggtaaca gcgaaaatcc
     9841 atcagactaa tcacgcgatt cacctgattt gtgcggcggc gtggtttggg ggattactgc
     9901 cggtgctctg gtgtatgcaa ctcatcaaag gccgttggcg acactaggct attcaggcgc
     9961 tgatgcgttt ttcctggtgc gggcattttg cggtgatcgg cgtactggca agcggcgtgc
    10021 ttaatgcatt gttgattacc ggatttcccc ccacgcttac gacttactgg ggccaactct
    10081 tattgctcaa agccatcctg gtgatgatta tggtggtcat tgcgctggct aatcgttatg
    10141 ttcttgtacc gcgcatgagg caggatgaag atcgtgcggc accctggttt gtgtggatga
    10201 ctaaactcga atgggcgata ggtgcggtag tgctggtgat catcagcctg cttgcgactc
    10261 tcgaaccttt ttgatggatt acgaaaatga tgaaaaaaag tattctggcg tttctgttac
    10321 tcaccagttc tgcagcggcg ctggcggctc cacaggtgat aaccgtgagt cgttttgaag
    10381 tgggtaaaga caaatgggcg tttaatcgcg aagaggtaat gctgacttgc cgaccgggta
    10441 atgctttgta tgtcatcaac ccaagtaccc tcgtgcagta tcctttaaac gatatcgcac
    10501 aaaaggaagt tgccagtggg aagaccaatg cccaacccat ttcggtgatt cagattgatg
    10561 atcctaacaa tcccggcgaa aaaatgagtc tggcaccgtt tatagaacga gctgaaaaac
    10621 tctgttaatt acctaaaata gccttttgat ttccaataaa aaaaccgcct cagttctttc
    10681 accagaacgg gcggttttta acatttaagc tgatgagcac cgcgcttttt attgaccatt
    10741 ttgcacgcaa actggaaaac ctggcgtcgt catctattct taaagggcaa ggcaactaag
    10801 cctgcattaa tgccaacttt tagcgcacgg ctctctccca agagccattt ccctggaccg
    10861 aatacaggaa tcgtattcgg tcttttttta attgtattta aaatcaatta gttgcaaacg
    10921 tctccccgaa attccccgaa atttactcga atttctgtat tccggtcttt tttggttata
    10981 tcacaaccaa aatacattta acaatccatt tacgttaaaa tcagagcagt aagtacgttt
    11041 tttctctctc atcaagatac atttttgttg tcttctccga tgtgtggcca agtagacgct
    11101 gagcaaattc ttctccacat atttctttgt acaatcgtcc agccaggctt ctgatttcgt
    11161 gaaaagttgg tgggttttca ctgaacagga tacctgttaa ttttctggct gcgacaaatt
    11221 tctttgtcag gccgtccggg tgaatgctgc cgtcagggct gttttttcta atcccggcac
    11281 tgattagata atccccccta cttaccatgc ggcactgttc aactactgca ccaagtcgta
    11341 aaccagcgac aggaaggctg agtgacaggg ggatagcaat catcattcct gtcttaattt
    11401 gcctgatgtg gagacgatca tcataaatat cactgaaccg catattcgtt atgtcttcgc
    11461 gacgttgtcc tgttacaagg gctaagtcca tagctaatgg gaaccatgcc ggaagttgat
    11521 ctgctgcctc cctgatgcag ttgtatgtct ttagtttcag ccgttctctt gtaactacta
    11581 ttttcggtgc tcttgttggc gttactggat tttgagatat acgtccttca acaatggctt
    11641 cgcggaacat atcagataac acagaacgca ttgatcctgc catcgtgttt ttccctcctt
    11701 caatctacaa atcaagaaac tcggcgatat ggcgagtggt tatttctgtc agtaaaaccc
    11761 ctcctaattt ttcttttatt gtctccagtt gatttacccg agttttataa gtatttctgg
    11821 acgcttttct ccttataaga atcgttttgt aacgttcaat ccagcctgcc atagtaaatg
    11881 agtcgacccc tttaagcttt tcaattaggg cagcaggaga gtagtttttg tatatataat
    11941 gatttgcttc aattgcctgc gctactgcat ctcttcttga aattttacct agtgtaaatt
    12001 cttctttcgt cagagggttg cgccagtaat atgctttgtc tctccttcga tatgttaagt
    12061 ttctaggcaa attgggatcg tattttttcc gctgcatgtt ttaacttctc cattaacgga
    12121 ctgtctctcc cttgtcgccc attaggctga tggtgtgtta tatcggtatc aaccttattt
    12181 gggttgatat agaaagcctc cggaaccacc ctgtaactcc tcccgtgtag ttcaggtgca
    12241 ggataaatgt ttccattcct tgcccatcgt ctcagcgttg atattgatgg tgggttatcc
    12301 ggatatctga gttttcccca cgttttgagt gtcacaagat tcattgccat acctctcacg
    12361 atatgaccgc cagtaaatat acagaatact ggcgggtgtg gttgattttt aata
//
LOCUS       DAJTQY010000105        12220 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.105, whole genome shotgun sequence.
ACCESSION   DAJTQY010000105 DAJTQY010000000
VERSION     DAJTQY010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12220
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.105"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(<1..493)
                     /locus_tag="OO349_003262"
     CDS             complement(<1..493)
                     /locus_tag="OO349_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5868229.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIAL
                     LSSA"
     gene            complement(524..1216)
                     /gene="fimC"
                     /locus_tag="OO349_003263"
     CDS             complement(524..1216)
                     /gene="fimC"
                     /locus_tag="OO349_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HCR5868230.1"
                     /translation="MMTKIKLLMLMIFYLIISASAHAAGGIALGATRIIYPADAKQTA
                     VWIKNSHTNELFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVSSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            complement(1436..1978)
                     /gene="fimA"
                     /locus_tag="OO349_003264"
     CDS             complement(1436..1978)
                     /gene="fimA"
                     /locus_tag="OO349_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HCR5868231.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIASSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
     gene            2459..3325
                     /gene="folD"
                     /locus_tag="OO349_003265"
     CDS             2459..3325
                     /gene="folD"
                     /locus_tag="OO349_003265"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HCR5868232.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            3327..3539
                     /gene="ybcJ"
                     /locus_tag="OO349_003266"
     CDS             3327..3539
                     /gene="ybcJ"
                     /locus_tag="OO349_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HCR5868233.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            3647..4156
                     /locus_tag="OO349_003267"
     CDS             3647..4156
                     /locus_tag="OO349_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR5868234.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKGLLFAGIILAMLPDADVLSFKFG
                     VAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSLLDSV
                     TTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTAYGHQVIISELMWVWLPGMLL
                     MGMLWWRRR"
     gene            complement(4192..5577)
                     /gene="cysS"
                     /locus_tag="OO349_003268"
     CDS             complement(4192..5577)
                     /gene="cysS"
                     /locus_tag="OO349_003268"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HCR5868235.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            5751..6245
                     /gene="ppiB"
                     /locus_tag="OO349_003269"
     CDS             5751..6245
                     /gene="ppiB"
                     /locus_tag="OO349_003269"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HCR5868236.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVDGMDVVDKIKGVATGRNGMHQDVPKEDVIIESV
                     TVSE"
     gene            6248..6970
                     /gene="lpxH"
                     /locus_tag="OO349_003270"
     CDS             6248..6970
                     /gene="lpxH"
                     /locus_tag="OO349_003270"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HCR5868237.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            7088..7597
                     /gene="purE"
                     /locus_tag="OO349_003271"
     CDS             7088..7597
                     /gene="purE"
                     /locus_tag="OO349_003271"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HCR5868238.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAVEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNGWRKAQTDEVL
                     ENPDPRGAA"
     gene            7594..8661
                     /gene="purK"
                     /locus_tag="OO349_003272"
     CDS             7594..8661
                     /gene="purK"
                     /locus_tag="OO349_003272"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HCR5868239.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWSLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYACGVIWAQSKFS"
     gene            complement(8798..9691)
                     /locus_tag="OO349_003273"
     CDS             complement(8798..9691)
                     /locus_tag="OO349_003273"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCR5868240.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRSKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(9688..10335)
                     /locus_tag="OO349_003274"
                     /pseudo
     CDS             complement(9688..10335)
                     /locus_tag="OO349_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
     gene            10338..11570
                     /gene="allC"
                     /locus_tag="OO349_003275"
     CDS             10338..11570
                     /gene="allC"
                     /locus_tag="OO349_003275"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HCR5868241.1"
                     /translation="MTHSRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFKK
                     RMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWLA
                     IDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNSF
                     ADAMKACGFTLPNAPLTPRQAIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTVT
                     LNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNVV
                     PGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKELV
                     ATLTELCEREKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITDL
                     AEGVKTLALMLYQLAWQK"
     gene            11581..>12220
                     /gene="allE"
                     /locus_tag="OO349_003276"
     CDS             11581..>12220
                     /gene="allE"
                     /locus_tag="OO349_003276"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HCR5868242.1"
                     /translation="MGYLNNVTGYREDLLANRAIVKHGNFALLTPDGLIKNIIPDFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLFSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVY"
BASE COUNT         2969 a         3156 c         3117 g         2978 t
ORIGIN      
        1 gagcgctact caacaacgct atctgaggaa tagtgatgtt caggcgcatc gctgcaaaat
       61 caaagttaat tgtggcatca ggtacagctt tcagtaaatt gatgcattta ttgttttgct
      121 gctgggctaa ctcgggaaat gcactgctat taaggccaat tcgttcaagg agtacctggt
      181 taaaacaggg cattaaccca cctgatttat cacctgtatt ttctgttgtc gattcaaata
      241 cgatatcctg tgaaccaatg aactcatcat tacgccagag atccacccga tacacgcccg
      301 caggttgatg attacctctc tcaaagcgcg ataaatcagc aacggatgtg ccattctcta
      361 ataaaaatgc cgggttgaaa taactttccg catagcttaa aaaggagggt aaaatggcaa
      421 gagatgaata acaaattccg gccagacacc aggtatacct ctgcggaata tccgtagtag
      481 tgggtatttt cataattagc tctgatattg tcagatgatt cggttagttt aagttcactt
      541 ctctgaccgg ggttactgaa ccataatcat taacggtctg aaatttcagc tttccctgca
      601 ctcctgaggg taagggaatt tgtgaactaa ctctgggtgc cgccatagca ttccccaact
      661 tttggctgcc cacttgcagg ttaacgaggg tgacataaaa aggtgatgga ttatgaacat
      721 tgatatagtt accggatcgc gaaaacttga gtgtgtccgg cgcttctgcg ggtaattctt
      781 gtaattgaat tggacggaga aataatttca tgcgcgataa aatcgccagt tgcaaaacat
      841 tcctgccgtt caatgcattt ttatctaccg aagggatcgt cttaacattc atccagaaca
      901 gagactcacg atctgccgcc agcggtggac cggtgtaaat aatacgcaaa gtattttcgc
      961 ttttgggttc actaacaaat agtggcggtg taatgatgaa tgacttttct tttacaccgc
     1021 tgctgttttc aatccacgaa ttgaccagaa agagctcatt ggtatggcta tttttaatcc
     1081 ataccgcagt ctgtttagca tcagcgggat aaataatacg cgtggcacct aatgcgatcc
     1141 ctccggcagc atgggcgctg gccgaaatga ttaaataaaa tatcatgagc atcaataact
     1201 ttattttagt catcatagtt aatacgcctt gtgtattcct gacggcaaaa ataaggagta
     1261 taaagaaatg attgctttgg cttcttacaa cacatcattc ccttttcatt taataaagcg
     1321 gcatgataca aagtgaacgt ggtttcgatt tacattgcca ccataccgct tcagacaacc
     1381 ggtacattac tttgttataa gacgtgatat ataattcaaa acaacgtggt tttgattatt
     1441 cgtatctcat aataaaagtc gcgtcagcat ttgcttgccc tgctgatgca cttgtaccgg
     1501 tgcctttata acgtgcagaa aaatgaagaa cgttggtccc ggggatcaag ttctggttgg
     1561 ttgagaagct attcccatca ggtttgagaa ttgcggatgt attatcaagt atttcaatac
     1621 cgacacccgt tgctgttgtt gtatttgtac tggatgcaat ggccaataaa ttatcattga
     1681 ttgcatcagc ctgaccagaa aatgcaacgg cagcattagc ggcaacaaca ggatcgcagt
     1741 cgttcaactg aatggtgaat ggaattaatg cagaggtatt accaacagca ttgaaaatat
     1801 cggtacgata ttgaccgagt gtgacaacct ggtctgccga gtcagtattc actgaacagg
     1861 cagcattcac cagttcgcct tcaaagtgaa ttgtaccgcc atctacaacg gcagcataac
     1921 tacccgtagc ggcgaatagt gcggcagcca gcgcagacga aataaatctt aatttcatat
     1981 atattccttc aatctcattt atcgactcca catccgtata taaccgatta ctttatttaa
     2041 gacactgata gtagtaaaat tcctttttat cctctaagaa tgtcttaatt gaaaatatgc
     2101 actctattct aaaaaataga gagccccgtt agatgaatac ttccgcgcaa aatatattca
     2161 acacaaatat agacctgaag cggtaaatta ccaggctgaa aattcttttt atattgtcag
     2221 gtatttctta aattatctta atccttaaac aaggaaataa atcagttcca gatttacaac
     2281 gccatcatgt acgaaaaatg aagctttcaa tctcagcgac ctcagcgacg gtgcgcctaa
     2341 ccttcgcaag aggtcgcttc acgcgataaa tctgaaacga aacctgacag cgcgccccgc
     2401 ttctgacaaa atagacgcat ccccttcgac ctacgtaaca gatggaatcc tctctctgat
     2461 ggcagcaaag attattgacg gtaaaacgat tgcgcagcag gtgcgctctg aagttgctca
     2521 aaaagttcag gctcgtattg cagccggact gcgggcacca ggactggccg ttgtgctggt
     2581 gggtagtaac cctgcatcgc aaatttatgt cgcaagcaaa cgcaaggctt gtgaagaagt
     2641 cgggttcgtc tcccgctctt atgacctccc ggaaaccacc agcgaagcgg agctgctgga
     2701 gcttatcgat gcgctgaatg ccgacaacac catcgatggc attctggttc aactgccgtt
     2761 accggcgggt attgataacg tcaaagtgct ggaacgtatt catccggaca aagacgtgga
     2821 cggtttccat ccttacaacg tcggtcgtct gtgccagcgc gcgccgcgtc tgcgtccctg
     2881 caccccgcgc ggtatcgtca cgctgcttga gcgttacaac attgatacct tcggcctcaa
     2941 cgccgtggtg attggcgcat cgaatatcgt cggtcgccca atgagcatgg aactgttgct
     3001 cgcaggttgc accaccaccg tgactcaccg cttcactaaa aatctgcgtc atcacgtaga
     3061 aaatgccgat ctgttgatcg ttgccgttgg caagccaggc tttattcccg gtgactggat
     3121 caaagaaggc gcaattgtga ttgatgtcgg catcaaccgt ctggaaaatg gcaaagttgt
     3181 gggcgacgtc gtgtttgaag acgcggctaa acgcgcctca tacattacgc ctgttcccgg
     3241 cggcgttggc ccgatgacgg ttgccacgct gattgaaaac acgctacagg cgtgcgttga
     3301 atatcatgat ccacagggtg agtaacatgg cgacattttc tttaggtaaa catccgcacg
     3361 ttgagctgtg cgacttgctg aaactggaag gctggagcga aagcggcgcg caggcgaaaa
     3421 tcgcgattgc cgaaggccag gtgaaagtcg acggtgcggt tgaaacgcgc aaacgctgca
     3481 aaatcgtcgc cggtcagaca gtgagttttg caggacacag cgtacaggtt gttgcctgat
     3541 ccctttcccg gcctgtatca acaggccggt tctcttttca gacttttgcc ctctacgccc
     3601 cgttcatcca tgctgatagc atcgacattt tgccgaagcg accatcatgc caaccgttat
     3661 tacgcacgcc gccgttcccc tttgcattgg tttagggttg ggttcgaaag gtctgttatt
     3721 tgccggaatc atcctggcga tgctgcccga tgccgacgta ttgtcgttta aatttggcgt
     3781 tgcttacggc aatgtttttg gtcatcgcgg ttttacccat tcgctggtgt ttgcgtttgt
     3841 tgtcccgctc ttatgtgtgc tgattggacg tcgatggttc agggctgggc tgattcgctg
     3901 ctggctgttt ttaaccgtct cgttgctatc gcacagctta ctggattcgg taaccactgg
     3961 cggtaagggc gttggctggc tgtggccgtg gtcagatgaa cgctttttcg ctccctggca
     4021 ggtgattaaa gtcgcgccgt ttgcattgtc tcgttacacc acagcgtacg ggcatcaggt
     4081 gattatttcc gaattgatgt gggtgtggtt gccggggatg ctactgatgg gaatgttgtg
     4141 gtggcgcaga cgataaccgg atgcgaaaac tcgcatccgg caattgcgca attacttacg
     4201 acgccaggtg gtcccttgcg ggccatcttc cagcacgatc cccatctcgt taagacggtc
     4261 acgtgccgca tctgccgccg cccagtcttt cgctttacgg gcatccagac gctgttgaat
     4321 taacgcttca atctcagcca cttcgctgtc gtctgcctgc gcgccgcttt gcaggaacgc
     4381 ttccggttct tgctccagca ggcccaatac agcggaaagt ttacgcaggt gagatgccat
     4441 tgcattcgcc gctgccatat cttctgcttt cagacggttc acttcacgcg ccatatcaaa
     4501 cagcacggaa taggcttccg gggtgttgaa atcgtcgtcc atcgcttcaa taaagcgcgc
     4561 ttcaaacgct tcgccaccgg caggcgcaac ggttttatct gtgccgcgca gcgcagtgta
     4621 gaggcgctcc agcgccgcac gcgcctgctt caggttctct tcgctgtagt tcagctggct
     4681 gcgatagtgg cccgacatca ggaagtaacg cacggtttcc gcgtcgtagt atttcagcac
     4741 atcgcgcacg gtaaagaagt tacccagcga tttggacatc ttctcgcggt caaccatcac
     4801 cattccggag tgcatccagt agttcacata ctgaccatca tgggcacagg tggactgcgc
     4861 gatttcgttc tcgtggtgcg ggaacatcag gtctgaaccg ccgccgtgga tatcaaagtg
     4921 gttacccagc tgcttgcagt tcatggccga acattcaatg tgccagccag gacgacccgc
     4981 gccccacgga gacggccagc tcggttcgcc ctctttcgac atcttccaca gaacgaagtc
     5041 catcgggttg cgtttgtcat caaccacgtc aacgcgcgcg ccagcctgca gctggtcgag
     5101 atcctgacgc gacagcacgc cataagttgg atcggtcggg acgtcgaaca tcacgtcgcc
     5161 gttgtccgcc acataagcgt gacctttggc gatcagttgt tcagtgagtt caataatttc
     5221 tgcgatatgg tgcgtcgcgc gcggctccat atccgggcgc agaatgttca aagcatcaaa
     5281 atctttgtgc atttcggcga tcatgcgatc caccagcgcc acaaagcttt cgccattttc
     5341 attggcgcgt ttgatgattt tgtcgtcgat atcggtaatg ttgcgcacat acttcagttt
     5401 atagccgagg aaacgcagat agcgcgcaac cacgtcaaaa gcaacaaagg tacgcccgtg
     5461 accgatatga cagagatcgt aaacggtgat tccacacacg tacatgccga cttccccggc
     5521 gtgaataggc ttaaattcct ctttttggcg tgtcagagta ttgaagattt ttagcatcga
     5581 agattccgtt tagacatgtg tgggtaattg agttgcgtat aatacccata tttcccgcgc
     5641 gaatcagcat acattgcgtg atgatccgat cgtgcggtta tgctataaca ccaccctata
     5701 tatgacccga actgggttga agcaccaatc aaacggaaca ggatgcaaaa atggttactt
     5761 tccacaccaa tcacggcgat attgtcatca aaacttttga cgataaagca cctgaaacag
     5821 ttaaaaactt cctggactac tgccgcgaag gtttttacaa caacaccatt ttccaccgtg
     5881 ttatcaacgg ctttatgatt cagggcggcg gttttgaacc gggcatgaaa caaaaagcca
     5941 ccaaagaacc gatcaaaaac gaagccaaca acggcctgaa aaatacccgt ggtacgctgg
     6001 caatggcacg tactcaggct ccgcactctg caactgcaca gttcttcatc aacgtggttg
     6061 ataacgactt cctgaacttc tctggcgaaa gcctgcaagg ttggggctac tgcgtgtttg
     6121 ctgaagtggt tgacggcatg gacgtggtag acaaaatcaa aggtgttgca accggtcgta
     6181 acggtatgca ccaggacgtg ccaaaagaag acgttatcat tgaaagcgtg accgttagcg
     6241 agtaatcgtg gcgacactct ttattgcaga tcttcatctc tgcgtggaag aaccggcgat
     6301 caccgccggt tttctgcgtt ttttagcggg ggaagcccgc aaggccgacg cgctgtatat
     6361 tcttggcgat ctgtttgaag catggattgg cgacgacgat cccaacccac tccatcgcca
     6421 gatggcggca gcgatcaaag cggtgtccga ttccggcgtt ccctgttatt tcattcatgg
     6481 caaccgtgat tttctgctcg gcaaacgctt tgcccgtgaa agcggcatga cattattgcc
     6541 ggaagaaaag gtgctcgaac tttatggtcg ccgggtgtta attatgcatg gcgacacgct
     6601 gtgcaccgat gacgcgggtt atcaggcttt tcgcgccaaa gtccacaaac cctggctgca
     6661 aatgctattc ctcgccctgc cgttgtttgt gcgcaaacgc attgccgcgc gaatgcgcgc
     6721 gaacagcaaa gaagccaaca gcagtaaatc gctggcgatc atggacgtta accaaaacgc
     6781 tgtggtcagt gcgatggaaa aacatcaggt gcaatggcta atccacgggc atacccatcg
     6841 cccggcggtg catgaactta tcgccaacca gcaacctgct tttcgcgtgg tactgggtgc
     6901 ctggcatacg gaaggttcga tggtgaaagt cacggctgat gacgttgagc tgattcattt
     6961 tccgttttaa aaaacccaca actttgctga tttcacagcc acgcaaccgt tttccttgct
     7021 ctctttccgt gctattctct gtgccctcta aagccgagag ttgtgcacca caggagtttt
     7081 aagacgcatg tcttcccgca ataatccggc gcgtgtcgcc atcgtgatgg ggtccaaaag
     7141 cgactgggct accatgcagt tcgccgtcga aatcttcgaa atcctgaatg tcccgcacca
     7201 cgttgaagtg gtttctgctc accgcacccc cgataaactg ttcagcttcg ccgaaagcgc
     7261 cgaagagaac ggttatcagg tgattattgc gggcgcaggc ggcgcagcgc acctgccagg
     7321 catgattgcc gccaaaacgc tggtgccggt gctgggcgtg ccagtacaga gcgccgcact
     7381 gagcggtgtc gatagcctct actccatcgt acaaatgccg cgcggcattc cggtgggtac
     7441 gctggcgatt ggtaaagctg gcgcggcaaa cgcggcatta ctggcagcac aaattctcgc
     7501 gactcacgat aaagagctac accagcgtct gaatggctgg cgcaaagccc agaccgacga
     7561 agtgctggaa aacccggacc cgcgaggtgc ggcatgaaac aggtttgcgt cctcggtaac
     7621 gggcagttag gccgtatgct gcgtcaggca ggcgaaccgt taggcattgc tgtctggccg
     7681 gtcgggctgg acgctgaacc ggcggcggtg ccttttcaac aaagcgtgat taccgctgag
     7741 atcgaacgct ggccggaaac cgcattaacc cgcgagctgg cgcgtcatcc ggcctttgtg
     7801 aaccgcgatg tgttcccgat tattgctgac cgtctgactc agaaacagct tttcgataag
     7861 ctccacctgc cgaccgcgcc gtggcagtta cttgccgatc gcagcgagtg gcctgcggtg
     7921 tttgatcgtt taggtgaact ggcgattgtt aagcgtcgca ctggtggcta tgacggtcgc
     7981 ggtcaatgga gtttacgcgc caatgaaacc gaacagttac cggcagagtg ttacggtgaa
     8041 tgtattgtcg agcagggcat taacttctct ggtgaagtat cgctggttgg cgcgcgcggc
     8101 tttgatggca gcaccgtgtt ttatccgctg acgcataacc tgcatcagga cggtattttg
     8161 cgcaccagcg tcgcttttcc gcaggccaac gcacagcagc aggcgcaagc cgaagagatg
     8221 ctgtcggcga ttatgcagga gctgggctat gtgggcgtga tggcgatgga gtgttttgtc
     8281 accccgcaag gtctgctgat caacgaactg gctccgcgtg tgcataacag cggtcactgg
     8341 acacaaaacg gtgccagcat cagccagttt gagctgcatc tgcgggcgat taccgatctg
     8401 ccgttaccgc aaccagtagt gaataatccg tcggtgatga tcaatctgat tggtagcgat
     8461 gtgaattatg actggctgaa attgccgctg gtgcatctgc actggtacga caaagaagtc
     8521 cgtccggggc gtaaagtggg gcatctgaat ttgaccgaca gcgacacatc gcgtctgact
     8581 gcgacgctgg aagccttaat cccgctgctg ccgccagagt atgcctgcgg cgtgatttgg
     8641 gcgcagagta agttcagtta agcaggtgct catttgattg ccggatgcgg cgtaaacgcc
     8701 ttatccggct taccgctccg gtacccattg taggcctgat aagacgcgtc aagcgtcgca
     8761 tcaggcatgg tgcaccgatt gccggatgct ctcatcacta cagcgaaata caggtccccg
     8821 cttcgcccgc cagcgtctct tcaattcgcg ataacgcccc aatccacgcg ggtttactac
     8881 ggcttctgac ataaccactc accgccgtta ccttcggccc catcgaacca tcggctttgg
     8941 caaatggagc taactcatcc ggtgtggcat ggcgaatggc acgttgctgc ggcgttcccc
     9001 agttttcata taccgcatca gcatcggtga ggatcaccag tccatcggca ttaatctgct
     9061 cggcgagcaa cgcagcggcg agatctttat caatcaccgc ttcactccct gccccatctt
     9121 ccgtcacagg cacaccgccg ccgccactgc aaatcaccac atgcccctct ttgagcaaca
     9181 actcgatggc ttcgctgtcg agaattttac gcggttgcgg agacgccacc acccggcgca
     9241 aatatttacc atcacgtttc atctgccagc cgtaagccgc ttccagtgcc tcttgttctt
     9301 ctggctgata aaccggacca ataaatttct ctggctgcaa aaacgccgga tcatcaggcg
     9361 aaacctcaat gcgcgtcagc accgtcgtaa caggcggcat ctgcggctgt gcgctcaagc
     9421 tctgcgccag catatagcca atcatcccct ggctttccgc caccagtaca tccagcggat
     9481 acggttctac ctctttccac gccagattct gtaacgccag cagccccacc tgcggcccgt
     9541 tgccgtgaac aatcgccagc cgataagaac gggccaggcg tgccagcgcg ggtacagcac
     9601 tggcgatatt gcgatattga ttttccgccg tcagcgcctc accgcgctgg agtaaggcgt
     9661 tgcccccaag agctacaacc agtgttttca tggttttcct tgtaataatt gttggccaag
     9721 ccagaacccc agcagcgtat ctgcccctga cgtatgcccc agcgccagca gcgaatcaat
     9781 cgcaacagcg gtacgtttcg ggcaactcag agcatggaca aagtgcagga gtggcgaggc
     9841 gaaatatcct tgcgcggcat aacgtaaata actgacgctc accgctgtgg taacgagttg
     9901 aagattgtcg gaacaggcaa aaaacgggcg accggcgcgc gcatctaaag cgccataata
     9961 ccaggccgcc agcagcattc cgctcaacgt gtcatcatga cttggcgtta atccggggcc
    10021 tttacccagc cagtaccgcc agtcggtctt aacgccattg agcgcggcct gaaaacagtg
    10081 acgaaactgg cgtaattcag cgggcagcgg atcgcttgcc gccatcgcca gtggcccgaa
    10141 aagtccggtt tcctccgcgc gttgcatcca tgcagctgca agcggttgag gatgcgaagg
    10201 cggcgtaata cgcagcaaac aataacgctg tggctgttta accgtgaaac gcccgaggcg
    10261 aatcccttga gccacaacct gtggtcgttc attgccgcat aatccaccgc ataacgcatc
    10321 gaattgcgtg cggcgaaatt acacattccc gtcaagctat agaagaaacg ctgccctggc
    10381 tttcctcttt tggcgctgac ccaacgggtg ggatgacccg tttactttat tcgccggaat
    10441 ggctggaaac ccagcagcaa tttaaaaaaa gaatggcagc aagtgggctg gaaacacgtt
    10501 tcgatgaagt ggggaattta tacggtcgcc tgagtggcac cgaatatcca caggaagtgg
    10561 ttctgagcgg ttcgcatatc gataccgtgg ttaacggcgg taacctcgac gggcaattcg
    10621 gcgcgctggc ggcgtggctg gcaattgact ggttgaaaac gcaatacggc gcaccgttac
    10681 gtacggtcga agtggtggcg atggcagaag aagaaggcag ccgcttcccg tatgtcttct
    10741 ggggcagtaa aaatatcttt gggctggcga atcctgacga cgtgcggaat atctgtgatg
    10801 ccaaaggaaa tagttttgcc gatgcgatga aggcttgcgg atttactctg ccgaacgccc
    10861 cactaactcc gcgtcaggct ataaaagcct ttgttgaact gcatattgaa cagggctgtg
    10921 tgctggaaag taatgggcaa tcaattggcg tggtgaatgc aattgtcggg cagcgtcgct
    10981 atacggtgac gctgaacggc gaatcaaacc atgcaggcac cacgccgatg ggttatcgtc
    11041 gtgatacggt ttacgctttc agtcgcattt gccatcagtc gatcgaaaaa gcgaaaaaga
    11101 tgggcgatcc gctggttctg acctttggca aagtagagcc gcgcccgaat acggtaaatg
    11161 tggtgccggg taaaaccacg ttcaccattg attgtcgtca taccgacgct gccgtgctgc
    11221 gcgatttcac ccaacagtta gaaaacgaca tgcgggcgat ttgcgatgaa atggacattg
    11281 gtattgatat cgatttatgg atggacgaag aacccgtgcc gatgaataag gagctggtcg
    11341 ccaccctgac agaattgtgt gaaagagaaa aactgaatta ccgggtgatg cacagtggtg
    11401 ccgggcacga cgcgcaaatt ttcgcgcctc gcgtaccaac ctgcatgatt tttatcccca
    11461 gcatcaacgg gatcagccat aacccggcgg aacgcaccaa tattactgac cttgccgaag
    11521 gggtcaaaac gttggcactc atgctttatc aacttgcctg gcagaaataa ggagtcataa
    11581 atgggatatt taaataacgt caccggttac cgcgaagatt tactggctaa ccgtgcgatt
    11641 gttaaacacg gtaatttcgc actgttaacc ccggatggtc tgataaaaaa tattattccg
    11701 gactttgaaa attgtgacgc gacaatcctc tccacgccaa agctgggtgc ctcttttgtt
    11761 gattatctgg tcacactgca tcaaaacggt ggcaaccaac agggcttcgg tggcgaaggc
    11821 attgaaacgt tcctgtatgt gatctctgga aatatcactg ccaaagccga aggcaaaaca
    11881 tttgccttaa gcgaaggtgg ctatctttat tgcccaccag gctccttaat gacgtttgtt
    11941 aacgcccagg ccgaagacag ccaaatcttt ttatataagc gccgctatgt tccggtagaa
    12001 ggccatgcac cgtggctgtt ttctggcaat gccagcgaac tggaacgcat tcactatgaa
    12061 ggtatggatg atgtgatcct gctggatttt ctgcccaaag agttaggttt tgatatgaac
    12121 atgcatatcc tctcttttgc accaggtgcc agccacggtt atatcgaaac acacgttcag
    12181 gaacatggtg cctatattct ttccggtcag ggggtttata
//
LOCUS       DAJTQY010000106        12062 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.106, whole genome shotgun sequence.
ACCESSION   DAJTQY010000106 DAJTQY010000000
VERSION     DAJTQY010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12062
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.106"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(4..380)
                     /gene="rnpB"
                     /locus_tag="OO349_003277"
     ncRNA           complement(4..380)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="OO349_003277"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(413..1558)
                     /gene="garK"
                     /locus_tag="OO349_003278"
                     /pseudo
     CDS             complement(413..1558)
                     /gene="garK"
                     /locus_tag="OO349_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
     gene            complement(1655..2545)
                     /gene="garR"
                     /locus_tag="OO349_003279"
     CDS             complement(1655..2545)
                     /gene="garR"
                     /locus_tag="OO349_003279"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HCR5868243.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGAIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(2575..3345)
                     /gene="garL"
                     /locus_tag="OO349_003280"
                     /pseudo
     CDS             complement(2575..3345)
                     /gene="garL"
                     /locus_tag="OO349_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
     gene            complement(3361..4695)
                     /gene="garP"
                     /locus_tag="OO349_003281"
     CDS             complement(3361..4695)
                     /gene="garP"
                     /locus_tag="OO349_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HCR5868244.1"
                     /translation="MILDTVDEKRKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVICFVLTALWIKLIHNPTDHPRMSEEELKFISE
                     NGAVVDMDHKKPGSAAASGLKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            5070..6641
                     /gene="garD"
                     /locus_tag="OO349_003282"
     CDS             5070..6641
                     /gene="garD"
                     /locus_tag="OO349_003282"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HCR5868245.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVEIASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMHCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            complement(6693..7517)
                     /gene="agaR"
                     /locus_tag="OO349_003283"
     CDS             complement(6693..7517)
                     /gene="agaR"
                     /locus_tag="OO349_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HCR5868246.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKYTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGDHASSL"
     gene            7766..9046
                     /gene="kbaZ"
                     /locus_tag="OO349_003284"
     CDS             7766..9046
                     /gene="kbaZ"
                     /locus_tag="OO349_003284"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HCR5868247.1"
                     /translation="MKHLTEMVRQHKAGKTNAIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCR
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKASALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            9069..9542
                     /gene="agaV"
                     /locus_tag="OO349_003285"
     CDS             9069..9542
                     /gene="agaV"
                     /locus_tag="OO349_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HCR5868248.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            9553..10332
                     /locus_tag="OO349_003286"
     CDS             9553..10332
                     /locus_tag="OO349_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HCR5868249.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGDTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAATW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            10322..11131
                     /locus_tag="OO349_003287"
     CDS             10322..11131
                     /locus_tag="OO349_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HCR5868250.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDRSIAAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRG"
     gene            11218..11652
                     /locus_tag="OO349_003288"
     CDS             11218..11652
                     /locus_tag="OO349_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR5868251.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGDQSQFIAIDFPETSSAALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            11649..>12062
                     /locus_tag="OO349_003289"
     CDS             11649..>12062
                     /locus_tag="OO349_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCR5868252.1"
                     /translation="MTHVLRARRLLTEEGWFDDHQLRVADGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHP"
BASE COUNT         2878 a         3250 c         3075 g         2859 t
ORIGIN      
        1 aacaggtgaa actgaccgat aagccgggtt ctgtcgtgga cagtcattca tctaggccag
       61 caatcgctca ctggctcaag cagcctaccc gggttcagta cgggccgtac cttatgaacc
      121 cctatttggc cttgctccgg gtggagttta ccgtgccacg gactgttacc agccgcgcgg
      181 tgcgctctta ccgcaccctt tcacccttac ctgatcccgc ttgcacgggc catcggcggt
      241 ttgctctctg ttgcactggt cgtgggtttc ccccccaggc gttacctggc accctgccct
      301 atggagcccg gactttcctc ccctccgccc gtctcccccg aagaggacga cgacgaagcg
      361 gcgactgtct ggtcagcttc ggcgcgcagt atagagggtt tgcgcgccct tgtcaccccg
      421 tgttgcgcat tccaatcgcc agtgtcgcgg caatattacg tgaagcacgg cagatattgt
      481 cataagcccc gcggaatgct tcgtccaacg tacctatgct ggtcaatacg ctgaagaccg
      541 catcaatgcc atgctgatgt acaacgccaa catcatcggt caggctaccc gcaatgccaa
      601 tcaccggttt atggtacttc ttcgccacgt ttgcgacgcc aatcggtacc ttgccgtgaa
      661 tactctggct gtcaatacgc ccttcaccgg tgatcaccag cgtacaatcg tgaatatgtt
      721 cctccagatt cagcgccgta gtgacgattt caataccact tttcagttcc gcaccaagaa
      781 acgccatcag caccgcaccc ataccgcccg cagctcctgc gccggggaca tcttttacat
      841 caacatgcag cgctttttta atgacatcgg catagtgaga gaggttattg tccagctcaa
      901 caatcatcgc ttcactggct cccttttgtg ggccaaagat gcgcgatgcg ccgttatcgc
      961 ccaccagcgg attggtgaca tcacaagcga cgcgaatgac gcaatctttt aagcgcggat
     1021 cgaggccgga aatatcaata tcattcagag tattaagact accgccgcca aaaccaattt
     1081 cattgccgtt ggcgtcgcat aatttcgccc ccagcgcctg taccatgcct gcgccgccat
     1141 catttgtagc gctaccgcca atgccgataa taatgtttgt cgcgccgctc tccagcgcct
     1201 acaggattaa ctcgcctgtg ccgcgtgaag tggtcacgag tggatcgcgt ttttccgcag
     1261 gtaccagctc cagcccactg gccgccgcca tttcaataaa cgcggttttg ccatcgccgg
     1321 agatccccca actggcattc actttctcgc ccagcggccc tgtaacccag gcgtgacgtt
     1381 cagaaccctg ggtggctgca atcatcgctt ccaccgttcc ttcgccaccg tcggcaaccg
     1441 gaatagaaac gtactgtgca tcaggaaaaa tttcccgaaa tcctttttct atcgcctgcg
     1501 caacctcgct ggcagataaa ctttctttat aagagtctgg ggcgattacg attttcatac
     1561 ctatgcctgt taccacatga cgccggaggg cgtttctctt attcggcctg gattccaggc
     1621 ccggattgca atacgccatc cgggcacgac gtcattaacg agtaacttcg actttcgcca
     1681 gtttttcgta gtagcacgcc agggcgctat gatccgccgt tcctaaacca tctgctcgca
     1741 gtgcctgcat catctccata accgcagctg tgagcggcag ttgcgcgccg acgccgtggg
     1801 aagtatccag cgcatttgcc agatccttaa tatgcagatc aatacggaag cccggcttga
     1861 agttgcggtc catcaccatc ggcgctttgg catccagcac ggtactgccc gccagtccac
     1921 cgcgaattgc ctgatacacc aggtccgggt taacgcccgc tttagttgcc agcgttaacg
     1981 cttctgacat cgcggcaata ttcagcgcca caatgacctg attcgccagt ttggtgacgt
     2041 tacctgcacc aattgccccg gtatgcacca cggaacccgc cattgctttc atcaaatcat
     2101 agtatttgtc gaaaatagcc ttgtcgccgc ccaccatcac tgacagcgta ccgtcgatgg
     2161 ctttcggttc accgccgctc accggagcat ccagcatatc aatgcctttc gctttcagcg
     2221 cttcgctgat ttcacggctt gccagcggtg cgatagaact catatcgatc aataccgtac
     2281 ctggcttcgc gccttcaata atgccattct cacccagcgc cacctctttc acatgagggg
     2341 agtttggcag catggttatg atgacgtcgc actgttcagc gatcgcttta gccgtagagg
     2401 ctgtttctgc acctgcagca atcacgtcag caatagcttc tgggttacgg tcagcaacca
     2461 ccagcgagta acctgctttc agaaggtttt tactcattgg tttacccata atccccaggc
     2521 caataaaacc aactttcata gtcatatcaa tcatctctct tgttgcggtg gtggttattt
     2581 tttaaaggta tcagccagtt tatgagtggc agagcggaag acgccgagat cgctgccgac
     2641 agccacaaac gtcgcgcccc attccagata acgacgcgca tcggcttcga ccggcgcgag
     2701 gataccgctg ggtttgccgt gcgcgctggc acggttaaaa atgtgctgaa ttgctttttg
     2761 tacatccggg tgtgatgcat tgccgagatg gcctaatgcc gcggccagat cgctggggcc
     2821 gacgaagatg ccgtctacgc cttcggtagc ggcaatggca tcgacgttat ctacgccctg
     2881 ctgactttct atctggacca gaatagtgat gttcttgttc gactgagcga aataatccgc
     2941 cacggtgcca aacatattgg cgcggtgaga aacggagacg ccgcgaatgc cttccggtgg
     3001 gtaacgggtt gatgccaccg cctgctctgc ttcctctttt gtttctacaa aaggaatcag
     3061 gaagttatag aaaccgatat ccagaagacg cttaataatt tccggctcgt tggtcggcac
     3121 tcacactact ggcgcgctgg cgctgccttt caaggccatt aactgcggaa taaacgtgga
     3181 gatatcgttt ggcgcatgtt cgccatccag caccagccag tcaaacccag ccaaaccaag
     3241 aacttcagtg ctaatcgggt tagagagtgc tgaccagcaa ccaatttgta cctgtttcgc
     3301 agccagtgcg gctttgaatt tattcgggaa aacatcgtta ttcatcgctt atacctttgc
     3361 ttatttctgc aattccatac gtttaatgtc gccaactacg aagaggtagc agaccatcgc
     3421 catcagcgct gaacatccca cgaaaaccag tgctgcattg aaggagtgca gttcacttac
     3481 caggtagcca atcaccagtg gagtgacaat ggaggcaaca ttgccaaaga cgttaaagac
     3541 gccgccacag aggccaacaa tctctttcgg cgcggtgtca gaaatcaccg gccagcccag
     3601 agcaccaaat cctttgccaa aaaaagccag cgccatcagc atgaccacca gcgtggtgtt
     3661 gttggtgtag ttacataaga tgatggtgga agccagcaac attcccagca caatcggtag
     3721 cttacgtgcc agggtcaggg ataaaccgcg tttgatcaga taatccgaga agacacctcc
     3781 cagcacgccg cccgcaaaac cacacagtgc cggaatcgag gcgaccagac ccactttcag
     3841 aatcgacatg cctttttcct gcaccagata aatcgggaac caggtgagga agaaccaggt
     3901 gatggtgttg ataaaatatt gtccgaaaaa tacgcccaac atcatgcggt tagagagcaa
     3961 ttgcttgatg taatgcagtt tgagaccgct tgctgccgcg ctgcccggct ttttgtggtc
     4021 catatcgacc accgcgccat tttcagagat aaacttcagc tcttcctcag acatacgtgg
     4081 gtgatctgtc gggttatgaa tcaacttgat ccacagcgcc gtcagcacaa aacaaatcac
     4141 ccccataacg gtaaagacgt gctcccatcc ccaggcgaaa gtcagccagc caagcagagg
     4201 cgaaaagagc gccagcgaga aatattgtgc cgagttaaag atggcggagg cagtaccacg
     4261 ttctttcgtc gggaaccagg cggcgacaat acgggcgttc gccgggaatg atggagcttc
     4321 cgagaatccc agcataaagc gcataaagaa catggagatc cctgcccagg ccagcgggaa
     4381 catatcaaca aagccttgca ggaaggtgaa cagcgaccag aaaaagaggc tgtaggtgta
     4441 aacttttttc gagccaaact tatcaagcaa ccagccgccg gggatttgca tcagcaagta
     4501 ggcccagcca aaagcggaga agatgtaacc catcgaaacc gcacttaact gcaactcttt
     4561 tgccacttcg gtaccagcaa tagacagcgt tgcacgatcg gcgtagttaa cggcggtaac
     4621 aataaaaata atcagtaata ttaaatagcg ggtatgcaca cctttccttt tttcgtcaac
     4681 ggtgtccaga atcattttat ttacctcggg tacttatgct gatttttatt attatgggga
     4741 aggtgttatt tataagtttc atttatgccg taacgacaat gaactcggga attagtataa
     4801 gcagcgcgag aataataatc attgtgcaaa tgctaattta attaatactt tttaaatatt
     4861 attttgagca tatgcacata aggttgcgcg ctaaagcaca gatttgcgct ttaccttgcc
     4921 gggcggcact gcaatccctg aaatgattga cattgatcac atttctgcgt ttaaactcct
     4981 gacattctta tttcacccaa tgaaatcatt tatttttaaa tgagaccagg tcctcatttt
     5041 aataacccct ggctggagaa tattgcacaa tggccaacat cgaaatcaga caagaaacgc
     5101 caactgcgtt ttatataaaa gttcacgaca cagataatgt ggcaattatt gttaatgata
     5161 atggcctgaa agcaggaacg cgttttccgg atgggctgga attaattgaa catattcccc
     5221 aggggcataa agtcgcattg ctggacattc cggctaatgg tgaaattatt cgttatggcg
     5281 aagtgattgg ttacgccgtg cgtgcaatcc cacgcggaag ctggatcgac gaatcaatgg
     5341 ttgtactacc ggaagcgccg ccgttacaca cgctgccact ggcaaccaaa gtcccggaac
     5401 ccttaccgcc gctggaagga tacacctttg agggctatcg caatgccgat ggcagcgtgg
     5461 gcaccaaaaa cctgctcggc atcaccacca gcgtccactg tgtggcaggc gtggtggact
     5521 atgtagtaaa aatcattgaa cgcgatctgc taccgaaata cccgaacgtc gatggcgtgg
     5581 tggggctgaa tcatttgtac ggttgtggcg tggcgattaa cgcaccggca gcagttgtac
     5641 ctattcgtac cattcacaat atttcgctga accctaactt tggcggcgaa gtaatggtga
     5701 ttggcctggg ttgtgaaaag ttgcagcctg agcgcctgct gactggaacg gatgatgtgc
     5761 aagctattcc agtagaaatc gccagcattg tcagtttgca ggatgaaaag catgtcggtt
     5821 ttcagtccat ggtcgaggat attttgcagg tcgccgaacg ccatctacaa aaactgaatc
     5881 aacgtcagcg agaaacctgt ccggcttcag aactggttgt cggcatgcat tgcggtggca
     5941 gcgatgcgtt ttctggcgta acggcaaacc cggcagttgg ctatgcgtct gatctactgg
     6001 tgcgctgcgg cgcaacggtg atgttttcag aagtcacgga agtgcgtgac gcgatccatc
     6061 tgctgacacc acgcgcagtg aacgaagagg tcggcaaacg gctgctggag gagatggagt
     6121 ggtacgataa ctatctcaat atgggaaaaa ccgaccgcag cgccaaccct tcgccgggca
     6181 acaagaaagg cggtctggca aacgtggtag agaaagcgct cggctccatt gctaaatcgg
     6241 gcaaaagtgc aattgttgaa gtgctgtcgc ccggtcaacg cccgactaaa cgcggattaa
     6301 tttacgccgc gacgccagcc agcgattttg tctgtggcac gcaacaggtg gcttcgggta
     6361 tcaccgtgca agtgtttacg accggtcgtg gtacgccgta cggcctgatg gcggtacccg
     6421 tcattaaaat ggcaacccgc accgagctgg cgaaccgctg gtttgattta atggatatta
     6481 atgcgggcac catcgctacc ggcgaagaaa ctattgaaga ggtgggctgg aagttgttcc
     6541 actttattct cgacgtcgcc agcgggaaga agaaaacctt ctcggatcaa tggggactgc
     6601 ataaccagct ggcggtgttt aacccagcac cggtgacctg ataattcaat tcgtattatg
     6661 cccgatagca cacgtttatc gggcatattg agtcacagcg aagacgcgtg atccccgacc
     6721 agaatcactt caaccccagc ctttcgcagt ccttccagac tatccgcagg aatgccttca
     6781 tcaacaatga tcatgtcgat acgttgagta tcaatgatct tatgtaaact ggaacgattg
     6841 aatttactgg aatcggtgac cacgatgatc cgttccgcaa cttcgcacat ccgacggttt
     6901 aaacgggctt catcttcatt atgtgtgctg acgccgcgct ccagatcgat cgcatctaca
     6961 ccaagaaaca gcatatcgaa gtggtaattt tgcagcgatt gctcagcctg atcgccgtaa
     7021 aaagattgcg actgacggcg caaatgcccg ccggtcatca gcagctcaac gccttccgct
     7081 tccagcaacg cattagccac gttcataccg ttggtcatcg caattacgtc agtgtacttg
     7141 cgcatcagac gagcaatctc aaaagtggtg gtcccggaat cgaggatcac ccgatgacct
     7201 ggctgaatca actcaacggc agctttcgca acgctgcgtt tcatcgcggt gttcagtgcg
     7261 cttttatctt ccactgatgg ctcgactgac ggcgtcgtgc tatcgcagat caacgcgcca
     7321 ccataggcac gcacagcgat cccctgcttt tccagaaacg ccagatcatt gcggatcgtc
     7381 acagtagata cgccatacaa tgccgacaga tcgttaacct gcacactccc ttgctgtcgc
     7441 agacgctgaa tgatctgttc tcgtcgctcg ctggtgcctg tcactcgctt ctcacctgaa
     7501 gcgtcggtat tactcatagt aagtcctttc gtaaaacttt cgtttcattt cgttttgcct
     7561 attaacgcct ttctattaag caaatgcaag cccaccttgc ccattggcgc aagctactct
     7621 cgtttcagtg actttcatta cgtttctttt gtgaatcaga tcagaaaact attatctttc
     7681 gttttatttt tatctcacca tgacgcagta tcaattgaaa caaaacgaaa gatcaatatc
     7741 gcagtaatct gaactggaga ggaaagtgaa acatctgaca gaaatggtga gacagcacaa
     7801 agcgggcaaa acaaatgcaa tttatgccgt ttgttccgca catccgctgg tgctggaggc
     7861 tgcaatccgc tacgccagtg caaaccaaac gccgttactg attgaagcaa cctccaatca
     7921 ggtagaccag ttcggcggtt ataccggaat gacgcccgcc gattttcgcg gctttgtttg
     7981 tcagctcgcc gactcgttga atttcccgca ggatgcgctg attctgggtg gtgaccatct
     8041 ggggccaaac cgctggcaaa acctgccagc cgctcaggca atggccaatg ccgatgattt
     8101 gattaaaagc tacgttgcgg caggattcaa aaaaatccac cttgattgca gcatgtcctg
     8161 tcaggacgat ccaattccct taactgatga catcgtggct gaacgcgccg cccgtctggc
     8221 gaaagtggcg gaagaaacct gtcgcgaaca ctttggcgaa gccgatctgg agtatgtcat
     8281 tggtaccgaa gtgccggtac ctggcggcgc gcatgaaacc ttaagcgagc tggcggtcac
     8341 cacgccggat gccgcccgcg ccacgctgga agcccatcgt cacgcctttg aaaagcaagg
     8401 tttgaatgcc atctggccac gcattattgc cctggtggtt caacccggcg tcgaattcga
     8461 tcacaccaac gttattgatt atcagcccgc caaagcgagc gccttaagcc agatggtcga
     8521 aaactacgaa acgctgattt tcgaagcgca ctctaccgat tatcaaacgc cgcaatctct
     8581 gcgccagctg gtgattgacc actttgccat tctgaaagtt ggcccggcgc tgaccttcgc
     8641 cctgcgtgaa gccctttttt ctctggcggc gattgaagaa gaactggtac cagcgaaagc
     8701 ctgttccggt ctgcgtcagg tgctggaaaa cgtgatgctc gaccgcccgg aatactggca
     8761 aagccactac cacggtgacg gcaacgcgcg tcgtctggcg cgtggttaca gctactcaga
     8821 tcgcgtgcgc tattactggc cggacagcca gattgatgac gctttcgctc atctggttcg
     8881 taatctggcg gattcaccaa ttccgctacc gctcatcagc cagtatctgc cgctgcagta
     8941 cgtgaaagtt cgctccggcg agctgcaacc gacgccacgg gaactcatta taaaccatat
     9001 tcaggacatc ctggcgcagt accacacagc ctgtgaaggc caataagcaa aacaaagagg
     9061 aacatgctat gccaaatatt gttttaagcc ggattgatga acgcttgatt cacggtcagg
     9121 tcggcgttca gtgggtcgga tttgcggggg caaatctggt gctggtagcc aacgatgagg
     9181 ttgccgaaga tccggtacaa caaaacctga tggaaatggt actggcagaa gggatcgccg
     9241 tacgtttctg gacgctgcaa aaagttatcg acaacattca tcgcgccgcc gatcgacaga
     9301 aaatcctgct ggtttgtaaa acacccgccg atttcctgac gctggtgaaa ggtggcgttc
     9361 cggtgaatcg cattaacgtt ggcaatatgc actacgccaa tggcaaacaa caaatcgcca
     9421 aaacggtttc tgtggatgcg ggcgatatcg cagcatttaa cgacctgaaa gccgctgggg
     9481 tggaatgctt cgttcagggc gtcccgacag agcctgctgt ggacctcttt aaattacttt
     9541 gagggattca tcatggaaat cagcctgttg caggcatttg cgttgggcat tatcgccttt
     9601 atcgctggcc tagatatgtt taacggctta acccatatgc accgcccggt ggtcctcggc
     9661 ccgttggtcg ggctggtact tggcgatctg cataccggaa ttttaaccgg cgatacgctg
     9721 gaactagtgt ggatggggct ggccccgctg gcgggcgcac agccgcctaa cgtgattatc
     9781 ggtactatcg tcggcacggc gtttgccatt actactggcg tgaaacccga tgtcgcggtc
     9841 ggtgtcgccg tacctttcgc tgtcgcagta cagatgggga ttaccttcct gttctcggtg
     9901 atgtctggcg tgatgtctcg ctgcgaccgg atggcggaga acgccgatac tcgcggcatt
     9961 gaacgcgtga actatctggc actgctggca ctcggcactt tctattttct ctgcgctttc
    10021 ctgcctatct acttcggcgc ggaacatgca aaaaccatca ttgatgtcct gccgcaacga
    10081 ttaatcgacg gcctcggcgt cgcgggcggc atcatgccag caatcggttt tgccgtgctg
    10141 ctgaaaatca tgatgaaaaa cgtctacata ccctacttca tcctgggctt tgttgccgca
    10201 acctggctca agttaccggt gctggctatt gctgccgccg cgctggcgat ggcgctgatc
    10261 gatctgctgc gtaaatctcc tgaaccgact caacctgcgg cacagaaaga ggaatttgaa
    10321 gatggcatct aatcaaacca ccctgccgaa cgtcagcgaa aatgaagaaa cactgctgac
    10381 tggcgtcaat gaaaacgtgt atgaagatcg gagcattgcc gcggagctga cgaaaaaaga
    10441 tatcaaccgc gtcgcctggc gttccatgct gttacaagcg tcttttaact acgaacgtat
    10501 gcaggcttct ggctggttgt acggtctgct gcctgctctg aaaaagatcc acactaataa
    10561 acgcgacctg gcgcgcgcca tgaaggggca tatgggtttc ttcaataccc atccgtttct
    10621 ggtgacgttt gttatcggca ttatccttgc gatggagcgt tctaagcagg acgttaacag
    10681 tattcagagc accaaaattg ccgtcggtgc gccgcttggc gggattggcg atgcgatgtt
    10741 ctggctaacg ctactgccga tttgtggcgg gataggcgca agcctcgcgc tacaaggctc
    10801 tattcttggc gcagtcgtct ttattgtgct gttcaacgtg gtacacttgg ggctgcgttt
    10861 tggtctggcg cattatgctt accgcatggg cgtggcggcg attcccctta ttaaagcgaa
    10921 taccaaaaaa gtcggccatg ctgcgtcaat cgttggaatg acggtaatcg gcgcactggt
    10981 ggcaacctat gttcgtttaa gcaccacgct ggaaatcacg gcgggcgacg cagtagttaa
    11041 gttacaggct gatgttatcg acaaactgat gcctgccttc ttaccgctgg tctacaccct
    11101 gaccatgttc tggctggtac gccgcggcta gagtccgctg cgcctgattg cggttaccgt
    11161 ggttctaggg atcgtcggta aattctgcca tttcctttaa atacaatgag gtattagatg
    11221 ttaagtatta ttttgacagg gcatggcggg tttgccagtg gcatggaaaa agcaatgaag
    11281 caaatcctcg gcgatcaatc gcagtttatt gccatcgatt ttcccgaaac ctcaagcgcc
    11341 gcgctgctta catcgcagct tgaagaggcc atcgcccaac tggattgtga agacggcatt
    11401 gtttttctga cggatttgct gggcggcacg ccgtttcgcg tagcttcaac gctggcgatg
    11461 caaaaaccgg gctgtgaagt gattaccggc accaatttgc aactgctact ggagatggtg
    11521 ctggagcgcg aagggttaag cggtgaagaa ttccgtgtac aggcgctgga gtgtggacac
    11581 cgagggctga ccagtctggt ggacgagtta ggccgctgcc atgaagaatg tccggtcgag
    11641 gaaggaatat gacacacgtt ctgcgcgcca gaaggctgct gactgaagag ggatggttcg
    11701 atgaccatca gttgcgtgtt gctgacggtg tcatcgcagc aatcgaaccg attccagtgg
    11761 gcgtgactga acgcgatgcg gaactgctct gccccgctta catcgacacc catgtacacg
    11821 gtggtgcggg cgttgatgta atggatgacg cgccggatgt actcgacaag ctggcaatgc
    11881 acaaggcacg cgaaggtgtc ggcagttggt taccgaccac cgtaaccgcg ccgcttaata
    11941 ccattcatgc ggcgctgaaa cgtattgctc aacgttgcca acgcggcgga cctggtgcgc
    12001 aagtgctggg gagttatctc gaaggaccgt acttcacgcc gcagaataaa ggcgcgcatc
    12061 cg
//
LOCUS       DAJTQY010000107        11923 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.107, whole genome shotgun sequence.
ACCESSION   DAJTQY010000107 DAJTQY010000000
VERSION     DAJTQY010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11923
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.107"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(<1..2338)
                     /locus_tag="OO349_003290"
     CDS             complement(<1..2338)
                     /locus_tag="OO349_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HCR5868253.1"
                     /translation="MDTQRFQSQFHWHLSFKFSGAIAACLSLSLVGTGLAKADDSLPS
                     SNYAPPAGGTFFLLADSSFSSSEEAKVRLEAPGRDYRRYQMEEYGGVDVRLYRIPDPM
                     AFLRQQKNLHRIVVQPQYLGDGLNNTLTWLWDNWYGKSRRVMQRTFSSQSRQNVTQAL
                     PELHLGNAIIKPSRYVQNNQFSPLKKYPLVEQFRYPLWQAKPVEPQQGVKLEGASSNF
                     ISPQPGNIYIPLGQQEPGLYLVEAMVGGYRATTVVFVSDTVALSKVSGNELLVWTAGK
                     KQGEAKPGSEILWTDGLGVMTRGVTDDSGTLQLQHISPERSYILGKDAEGGVFVSENF
                     FYESEIYNTRLYIFTDRPLYRAGDRVDVKVMGREFRDPLHSSPIVSAPAKLSVLDANG
                     SLLQTVDVTLDARNGGQGSFRLPENAVAGGYELRLAYRNQVYSSSFRVANYIKPHFEI
                     GLALDKKEFKTGEAVSGKLQLLYPDGEPVKNARVQLSLRAQQLSMVGNDLRYAGRFPV
                     SLEGSETVSDASGHVALNLPAADKPSRYLLTVSASDGAAYRVTTTKEILIERGLAHYS
                     LSTAAQYSNSGESVVFRYAALESSKQVPVTYEWLRLEDRTSHSGELPSGGKSFTVNFA
                     KPGNYNLTLRDKDGLILAGLSHAVSGKGSTAHTGTVDIVADKTLYQPGETAKMLITFP
                     EPIDEALLTLERDRVEQQSLLSHPANWLTLQRLNDTLYEARVPVSNSFAPNITFSVLY
                     TRNGQYSFQNAGIKVAVPQLDIRVKTDKTHYQPGELVNV"
     gene            complement(2272..2895)
                     /locus_tag="OO349_003291"
     CDS             complement(2272..2895)
                     /locus_tag="OO349_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HCR5868254.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSRLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGLSSQYLPPEMTLTPEQRQLAQNW
                     NQGSGKNGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            complement(2892..4580)
                     /locus_tag="OO349_003292"
                     /pseudo
     CDS             complement(2892..4580)
                     /locus_tag="OO349_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
     gene            complement(4729..7356)
                     /gene="gyrA"
                     /locus_tag="OO349_003293"
     CDS             complement(4729..7356)
                     /gene="gyrA"
                     /locus_tag="OO349_003293"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HCR5868255.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            7503..8225
                     /locus_tag="OO349_003294"
     CDS             7503..8225
                     /locus_tag="OO349_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HCR5868256.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            complement(8353..10284)
                     /gene="yfaL"
                     /locus_tag="OO349_003295"
     CDS             complement(8353..10284)
                     /gene="yfaL"
                     /locus_tag="OO349_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005046855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HCR5868257.1"
                     /translation="MEITANVDTRSTTTEHGRDIEMRADGEVAVDAGVDTQWGALMAD
                     SSGQHQDEGSTFTKTGAGTLELTASGTTQSAVRVEEGTLKGDVADIFPYASSLWVGDG
                     ATFVTGADQDIQSIDATSSGTIDISDGTVLRLTGQDTSVALNASLFNGDGTLVNATDG
                     VTLTGELNTNLETDSLTYLADVTVNGDLTNTSGAVSLQNGVAGDTLTVNGDYTGGGTL
                     LFDSELNGDDSVSDQLVMNGNTAGNTTVVVNSITGIGEPTSTGIKVVDFAADPTQFQN
                     NAQFSLAGSGYVNMGAYDYTLVEDNNDWYLRSQEVTPPSPPEPDPTPDPDPTPDPDPT
                     PDPAPTPAYQPVLNAKVGGYLNNLRAANQAFMMERRDHAGGDGQTLNLRVIGGRYHYT
                     AAGQLAQQEDTSTVQLSGGLFSGRWGTDGEWMLGAVGGYSDNQGDSRSNMTGTRADNQ
                     NHGYAVGLTSSWYQHGNQKQGAWLDSWLQYAWFNNDVSEHEDGVDRYHSSGIIASLEV
                     GYQWLPGRGVVIEPQAQVIYQGVQQDDFTAANHARVSQSQGDDIQTRLGLHSEWRTAV
                     GVTPTLDLNYYHDPHATEIEEDGSTISDDAVKLRGEIKVGVTGNISQRVSLRGSVAWQ
                     KGSDDFAQTAGFLSMTVKW"
     gene            complement(10297..11892)
                     /locus_tag="OO349_003296"
     CDS             complement(10297..11892)
                     /locus_tag="OO349_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000183628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AidA family protein"
                     /protein_id="HCR5868258.1"
                     /translation="MTNNASGGAVFLQQGAEFSLLPENETGMTLFANNTVTGEYNNGG
                     AIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAVDLRVTNAINNIANDGKGG
                     AIYTINNDVYLSDDVFNNNQAYTSTSYSDGDGGAIDVTDNNSDSKHPSGYTIINNTAF
                     TNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSAAGYGDGPSTAAGG
                     FMYLGLSEVTFDIADGKTLVIGNTENDGAVDSIAGTGLITKTGSGDLVLNADNNDFTG
                     EMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDQYQNQAELNVGSTQQTF
                     VHALTGFQNGTLNIDAGGNVTVNQGSFAGIIEGAGQLTIAQNGSYVLAGAQSMALTGD
                     IVVDDGAVLSLEGDAADLTALQDDPQSIVLNGGVLDLSDFSTWQSGTSYNDGLEVSGS
                     SGTVIGSQDVVDLAGGDNLHIGGDGKDGVYVVVDASDGQVSLANNNSYLGTTQIASGT
                     LMVSDNSQLGDTHYNRQVIFTDK"
BASE COUNT         2757 a         3308 c         2947 g         2911 t
ORIGIN      
        1 cgacattgac cagttcacca ggctggtaat gggttttgtc cgttttcacc cggatatcaa
       61 gctgaggaac ggcaactttg atcccggcgt tctgaaaact gtactgacca ttacgggtat
      121 acagcaccga aaaagtgatg ttaggcgcaa aggaattgct cactggtacc cgggcttcat
      181 acagggtatc gtttaaacgt tgtagcgtta gccagtttgc cggatgcgaa agcagcgact
      241 gctgttccac gcgatcgcgt tccagcgtca ataacgcttc atcaattggc tccgggaaag
      301 taatcagcat cttcgcggtt tcgcctggct ggtacagcgt tttatccgcc acgatatcta
      361 ccgtaccagt atgcgccgtg ctgcccttac cgctgacggc atgacttaac ccagcgagaa
      421 ttaagccgtc tttatcgcgt aatgtcagat tgtagttgcc aggtttagcg aaattgacgg
      481 taaaggattt gccgcctgac ggtagctctc cgctatggct cgtgcggtct tcgagacgca
      541 accattcata cgtaacagga acctgttttg aagattccag cgcggcataa cggaacacaa
      601 ccgactcgcc gctattactg tattgtgcgg cggtacttaa agagtaatgc gccaggccgc
      661 gttcaatgag gatctctttg gtggtggtga cgcgatacgc cgcgccgtca ctggcggaga
      721 cggttaacaa atagcggctc ggtttatcgg cggcggggag atttaacgcc acatgaccgc
      781 tggcgtcgga caccgtttcg ctgccttcca gcgacacggg gaaacgtccg gcataacgca
      841 aatcgttacc gaccattgat aattgctgag cgcgcaaact taactgcacg cgggcatttt
      901 ttaccggctc gccatccggg tagagcagtt gcagtttgcc gctgaccgct tcgccagttt
      961 tgaactcttt tttgtcgaga gctaaaccaa tctcgaaatg tggcttgatg tagtttgcca
     1021 cgcgaaaact gctgctatag acctgattgc ggtaagcaag acgtaactca taacctccgg
     1081 caacggcatt ttctggcagg cggaaacttc cctgcccgcc attgcgcgca tccagcgtga
     1141 catcgacggt ttgcaacaga ctgccgttag cgtccagcac cgaaagcttc gccggggcgc
     1201 tgacgatggg ggatgaatgc aacggatcgc ggaactcgcg gcccatcact ttaacatcga
     1261 cacgatcgcc tgcgcgatat agcgggcgat cggtaaaaat atacaagcgg gtgttgtaga
     1321 tttcgctttc gtagaagaag ttctcggaga caaaaacgcc gccttcagca tccttaccca
     1381 gaatgtatga acgttctggc gatatatgtt gtaactgcaa agtaccgctg tcatcggtca
     1441 ccccgcgggt catcacgcca agaccgtcag tccacaagat ctctgagccg ggcttcgctt
     1501 caccctgttt tttacccgcg gtccatacca gaagctcgtt acctgacact ttgctaagcg
     1561 ccacggtatc ggaaacaaac accaccgtcg tcgcccgata cccacctacc atcgcctcga
     1621 cgaggtacag ccccggctct tgttggccga gaggaatata aatattaccc ggctgcggcg
     1681 agatgaaatt gctggatgcg ccttccagtt ttaccccttg ctgcggctcg accggtttag
     1741 cctgccatag tggataacgg aactgttcca ccaagggata ttttttcagc ggggaaaact
     1801 ggttgttctg tacataacgg gaaggtttaa taatggcatt gccgagatgt aattcgggta
     1861 atgcctgagt cacattctgc cgtgactgag aagagaaagt acgctgcatc acgcggcgag
     1921 atttgccgta ccagttatcc cacagccagg tcagcgtatt gttcagcccg tcgcccagat
     1981 attgcggttg caccacaatg cgatgcaggt ttttctgctg gcgcaaaaat gccatcgggt
     2041 caggaatacg atataggcga acgtccacgc cgccgtactc ttccatctga tagcgccgat
     2101 aatcacgccc cggcgcttcc agtcgcactt tcgcctcttc actgctgcta aaactgctgt
     2161 cagcaagcaa aaagaatgtt cccccggcgg gcggcgcata gttactggaa ggaagcgaat
     2221 catcagcttt agcaagccct gttcccacaa gagacaggga caagcacgct gctatcgcgc
     2281 cagaaaattt aaacgataaa tgccaatgaa attgggattg gaatcgttgg gtatccatcg
     2341 ggtgtccttc catgtcataa gttgttgcag actgactgcg cgcattccgt tgtcagtttt
     2401 cgtggcgctt ccggtgtggt agatgacgta acgccccatc cagaccatta agtgctgggc
     2461 atcgccctga tcgaaaaaaa tcatatcgcc aggcagcgcc tggtttatgt cttggccaat
     2521 aaactggctg ttgtactgaa tcaaattaat cgcggtcacg taggggccgt ttttcccgct
     2581 cccctgattc cagttttgcg ccagttgacg ctgttcaggt gttagcgtca tctctggcgg
     2641 caaatactgg ctagataaac cgttactttt tagccattta ctgtcgtgaa ctttcagcgt
     2701 ctcgttcgcc gcaaatcgca ccaggcccgc acaatcctgc tgataccagc gtggacttgg
     2761 cccctggcgg agctgttctt gtgcaatgcg tacaaaccag gcgcgaaaca gcctggattg
     2821 ttcgacattc agcatttcgc tatgggcaac aacacaacac agccagcaaa tcagcgccag
     2881 cagcccgtgt ctcatagcgg ctgccaggtt atgggtagcc actgccaggc gacaccgggt
     2941 tccatctggg ccagcttcat gacataacgc ggttgttgag ataaagcgtc cagcttcggc
     3001 attaataaag tttgtgcggc gttataaaaa accggttcga gattcttcgg cagactggtc
     3061 aacgtttcgt tacgcagcag tttcgccacg ccttgtggat tgatatagag cggaacgatg
     3121 ccatcagtgg gtattacatc caccattgcc gggcgggttt tattcagtgt ttgcagggcg
     3181 ttattaacca gcgtgtcatc gagggagaaa agcagcgttt tgttttgcat cgccagcgac
     3241 acacggaaaa aataatccga cattaattga tcgggttgcg ccgcctgcgc tttcggatac
     3301 tgaccgtatc gggaactcac ttcgcgacac cagatttgcg cttcgccctg ctgagtctgg
     3361 cttaccggca aaacaccttc tggcgctttg ctttcgtgcg caccaatatt ttgcgtaaac
     3421 agtttccctg gcaattgcgc ctgttcggca gtgccatcaa actgaccgac aaacagcggg
     3481 gtttgcaatt ttgagtcttc ataccagcac agccccgcgg caccgtctaa cgccccattc
     3541 agcttgtcgt tttcctggct gatgtgcgaa agcatctctt cggcaatacc gtgtgaatac
     3601 ggcaccgcca cacagaagct ggctccggca ggcatactgt tccagaccgg cgtaaaatcg
     3661 aaactggcat ctacgctggc ggattcatca tttaacgcca caaaactgtg ccagccgtcg
     3721 ttacccattt cgaagcgtac gccagcaaaa gaaggcatta accgctggta gccaaaaccc
     3781 agcaacctgg cgctgaccac gatgcgcagg cgtactggcg ttttttcggc tgtacgctct
     3841 tccaggccaa agctggcttg ccagcgtttt ttaccgctca acaaatcact tgcgatcgcc
     3901 gtggcttcgg tatcctgctg atcatcttta aacaacatat ccgtactgga aaacaccagc
     3961 atcttgtcct gatatgtcgc gaacatcagg gcgttattgc cgttatagcg caactgataa
     4021 acaggaatag tttcactatt tatcttgatg ctactgattt ccgttttgct taactagctg
     4081 tcgctggtag cggcaaacaa caatggctcc agcagtttgc ttaaaccact gcgctggatc
     4141 agcaccatgt aatgtgaaag atggccttgt ttatcgtgcc atagcgccgc ctgcgcgggc
     4201 tgatctaaga gtgacgaaaa gagcttatct ttcagcgtga gatcgtgttc atagacaatg
     4261 cggcgaatgc tgccttcaat gcccagacga tcggcatgat tctgataata gaaaacgaaa
     4321 tcttcgctca gaacatcgtg gagaaacgga atggtgagga gatctttggg aagctggctc
     4381 agagagtcgc tgtctagaaa gaggtccggc tcattgagat cgatttgcag attgttgtgc
     4441 accaccagcg gcgacaacgt tttttctggc ccactgccag catattgcaa cgcccagacg
     4501 ccagcggaaa gcagtgctat tgcgccaaaa cctacaagac catagaaccg ccagcctttc
     4561 gccttttttt caccactcat tgccacattc cttgtgtata gccagccatt ttttacgggc
     4621 acagccaaac tttaccgcgc cctaatacga caaaagccca gactttgcag cctggacttt
     4681 tcaattcaaa caagggagat agctcccttt tggcatgaag aagtaaaatt attcttcttc
     4741 tggctcgtcg tcaacgtcca cttccggagc aatttcatca tccccttccg cggcactgcc
     4801 gtcgatggta tccagatctt cctcgtcaac cggttcagca acacgctgca gacccactac
     4861 gttttcatct tccgcagtac ggatgaggat cacgccctgg gtgttacggc ccacgatgct
     4921 gatttctgaa acgcgagtac gtaccagcgt accggcatcg gtgatcatca tgatctggtc
     4981 gcagtcatct acctgcaccg cgccaaccac taaaccgtta cgttcggtaa ccttgatgga
     5041 gataacccct ttcgtcgcac gcgacttggt tgggtattcc gccactgcag tacgtttacc
     5101 gtaaccgttt tgcgttgcgg tgaggattgc gccatcgcca cgaggcacga tcagagagac
     5161 gactttatcg ccttcaccta agcgaatacc gcgcacacca gtggtattgc agcccatcgc
     5221 acggacagaa gactctttaa agcgcaccac tttaccttcg gcggagaaca gcattacttc
     5281 atcttcgcca ctggtcagat caacgccgat cagctcatcg ccgtcaacca gtttgatcgc
     5341 cactttaccg gcggtacgca gacggttgaa ttcggtgagg acggttttct tcacagtacc
     5401 gttagcggtc gccatgaaga ctttcacgcc ttcttcaaac tcggtcaccg gcaggatcgc
     5461 ggtgatacgt tcgtcctgtt ccagcggtag caggttgacg atcggacgac cgcgcgcgcc
     5521 acgagtggct tccggcaact ggtaaacttt catcgaatag acgcgaccac ggctggagaa
     5581 gcacagaata tggtcgtgcg tgttcgccac cagcagtcgg tcgataaagt cttcttcttt
     5641 aatacgtgcg gcagatttac ctttcccgcc acgacgctgc gcttcgtatt cagaaagcgg
     5701 ctgatattta acgtagccct ggtgagagag cgtcacgacc acatcttcct gggtgatcag
     5761 atcttccagg ttgatgtctg cgctgttggc ggtgatttca gtacgacgtt tgtcaccgaa
     5821 ctgttcacga accagctcca gctcttcgcg gatcacttcc atcagacgat cggcgctacc
     5881 aagaatacgc aacagttccg cgatctgatc cagcagctct ttgtattcgt cgagcagttt
     5941 ttcgtgctca aggccggtca gtttctgcaa acgcagatcc agaatcgcct gagcttgctg
     6001 ttcggtcagg tagtacagac catcacgcac gccgaactca ggctccagcc attccggacg
     6061 cgcagcatcg tcgccagcac gttccaacat ggcggcaacg ttgcccagct gccacggatt
     6121 agcaaccagc gcagttttcg cttctgcagg cgtcggcgca tgacggatca gttcgatgat
     6181 cgggtcaatg ttcgccagcg ccacggctaa tgcttcaagg atatgagcac gatcgcgagc
     6241 tttacgcagt tcgaaaatag tacgacgggt caccacttca cggcggtgac gaacaaacgc
     6301 cgcgatgatg tctttcaggt tcatgatctt cggctgacca tggtgcaatg ccaccatgtt
     6361 gataccgaaa gaaacctgca actgggtctg ggagtagagg ttgttgagca caacttcacc
     6421 gaccgcatcg cgtttcactt caatcacgat gcgcataccg tctttgtcag actcgtcacg
     6481 cagcgcgctg atgccttcca cgcgtttttc ttttaccagt tccgcaatct tctcgatcag
     6541 gcgcgctttg tttacctgat acggaatttc gtggacgata atggtttcac gtccggtttt
     6601 ggcgtcaact tccacttcag cacgggcgcg gatatatacc ttgccgcgac cggtacggta
     6661 agcttcttca ataccgcgac gaccgttaat gattgccgcc gtcgggaagt ccggccccgg
     6721 gatgtgttcc atcagccctt caatgctgat gtcttcatca tcgatatacg ccagacaacc
     6781 gttgatgact tccgtcaggt tgtgcggcgg gatgttggtt gccataccta cggcgatacc
     6841 ggaagaaccg ttcaccagca ggttaggaat tttggttggc atgacgtcgg gaattttttc
     6901 cgtgccgtca tagttatcaa cgaaatcgac cgtctctttt tcgagatcgg ccatcagttc
     6961 atgggcaatt ttcgccagac ggatttccgt ataacgcatt gccgccgcag agtcgccgtc
     7021 gatggaaccg aagttaccct gaccgtctac cagcatgtaa cgcagcgaga atggctgcgc
     7081 catacggacg atcgtgtcat aaaccgccga gtcaccatgg ggatggtatt taccgattac
     7141 gtcaccaacg acacgggcag attttttata ggctttgttc cagtcattgc ctagtacgtt
     7201 catggcgtaa agtacgcgac ggtgtaccgg cttcaggcca tctcggacat ctggcagcgc
     7261 acggccaaca atgaccgaca tcgcataatc cagataggag ctctttagct cttcctcaat
     7321 gttgaccggt gtaatttctc tcgcaaggtc gctcatctaa ccgctatccc tctactgtat
     7381 cccggattca aaggtcgcaa attataacac agccgcgcag tttgaggtaa acctatacgc
     7441 tttattcaca tccaatgcct gatatactcg tttgtcttgc caattacgga gtagaagtgc
     7501 caatgaatgc cgaaaaatcg ccggaaaacc ataacgtaga ccacgaagag atcgctaaat
     7561 ttgaagccgt cgcctcccgc tggtgggatc ttgaaggtga gttcaaaccg ttgcaccgca
     7621 ttaacccgct gcgtctgggc tatattgccg agcgtgctgg cggtttattt ggcaaaaagg
     7681 tgctcgatgt cggttgtggc ggcggcattc tggccgagag tatggcgcgc gaaggcgcga
     7741 cggtgaccgg tctggatatg ggctttgagc cattgcaggt ggcaaagctg cacgcgctgg
     7801 aaagcggcat tcaggtggat tacgtgcagg aaaccgtgga agagcacgcg gcaaaacatg
     7861 ccgggcagta tgatgtggtg acctgcatgg agatgctgga gcacgtaccc gatccgcagt
     7921 cagtggtcag agcctgtgcg caactggtga aaccaggcgg cgatgtcttt ttctcgacgc
     7981 ttaaccgcaa cggcaagtca tggctgatgg cggtggttgg cgcggaatat attttgcgca
     8041 tggtgcccaa aggtacgcat gatgtgaaga agtttattaa accggcagag ctgcttggct
     8101 gggtggatca gaccagttta aaagagcgac atataaccgg gctacattac aacccgatca
     8161 ctaattcctt taagctcggc cccggcgtgg atgtgaacta tatgctgcac acgcagaata
     8221 agtgaggttg atgtttggcc gcgccaatgc ctgatgcgac gcttgcagcg tcttatcagg
     8281 cctacaaatg ctctccgtag gccagataag gcgtttacgc cgcatccggc aattgtgatg
     8341 gtcagacagc gattaccatt tcaccgtcat cgacaaaaac cctgccgtct gggcaaaatc
     8401 atcactccct ttctgccacg ccacgctgcc gcgcagggac actcgctgac tgatattgcc
     8461 cgtgactccc acttttattt caccccgtag cttcaccgca tcgtcactga tagtgctgcc
     8521 atcttcttca atttccgtcg catggggatc gtgatagtaa ttcagatcta atgttggtgt
     8581 gacaccaacc gcggtacgcc attcgctgtg taaacccagc cgcgtctgaa tatcatcccc
     8641 ctgcgattgt gacacgcgcg catggttagc ggcggtaaaa tcatcctgct gcacgccctg
     8701 ataaatcacc tgcgcctgcg gttcaatcac cacaccacgc cccggtaacc actgataccc
     8761 gacttccagc gaggcgataa tccccgacga atggtaacga tccacgccat cttcatgttc
     8821 ggaaacatca ttgttaaacc acgcgtattg cagccagcta tccagccagg ccccctgctt
     8881 ctgattaccg tgctgatacc agcttgaggt cagcccaacg gcataaccgt ggttctggtt
     8941 atcggcgcga gttccggtca tattcgagcg gctgtcgccc tgattatcac tgtagccacc
     9001 aaccgcccca agcatccact cgccatccgt gccccagcgc ccgctaaaca agccgccgct
     9061 aagctgcacc gtagaagtgt cttcctgctg agccagttgc cctgccgccg tgtaatgata
     9121 acgtccgccg ataacacgta aattcagcgt ctgaccatcg ccaccagcgt gatcgcgtcg
     9181 ctccatcata aacgcctgat ttgccgcccg caggttattg agataaccgc caactttggc
     9241 attcagcacc ggctggtaag caggcgtagg ggccgggtcg ggggttgggt caggatccgg
     9301 cgtgggatca ggatcgggag tcgggtctgg ctcaggtggc gatggcggcg ttacttcttg
     9361 cgatcgcaga taccagtcgt tgttatcttc cactagcgtg tagtcatacg ctcccatgtt
     9421 gacgtagcca ctgccagcca gactgaactg tgcattgttt tgaaactgcg tgggatcagc
     9481 tgcgaaatca accactttaa tgcctgtcga tgtcggctca ccaatccctg taatggagtt
     9541 aaccaccaca gttgtgttgc cagcagtatt accgttcatc accaattgat cgcttaccga
     9601 gtcatcgccg tttaattcgc tgtcgaatag tagcgtaccg ccgccggtat aatcaccgtt
     9661 taccgtcagc gtatcgccag cgacgccatt ttgcaggcta accgcaccgg acgtattggt
     9721 cagatcacca ttaaccgtca cgtcagcaag ataggtcagg ctgtcagttt caaggttggt
     9781 attaagctcg cctgtcaacg tcacgccatc ggtagcattt accagcgtcc catcgccgtt
     9841 aaatagtgag gcattaaggg cgacggaagt atcctgcccg gtcagacgca aaaccgtacc
     9901 atcgctgatg tcgatagtgc cgctggaagt agcatcaatt gactgaatat cctgatccgc
     9961 gccagtaacg aacgttgccc catcaccaac ccacagtgac gaagcataag gaaagatatc
    10021 cgcaacatcg cctttcaggg tgccttcttc gacacgtacc gccgactgcg ttgtaccgct
    10081 ggcggtcagc tccagcgtac ccgcccccgt tttagtaaat gtgctaccct catcctgatg
    10141 ctgcccgctg ctgtcagcca tcagtgcgcc ccactgcgtg tctacccccg catcaactgc
    10201 cacttcaccg tcggcgcgca tttcaatatc acgcccatgc tcagtcgtcg tagagcgagt
    10261 atcgacattg gcagtaatct ccatcacgct ttcttgttac ttatcggtaa agataacctg
    10321 gcggttatag tgggtatctc caagctgcga gttgtcgctc accatcagcg taccggaggc
    10381 gatttgtgtt gtgcccaaat aactattatt gtttgccaga cttacctgcc cgtcgctcgc
    10441 atcgaccacc acgtagacgc catctttccc gtcgccgccg atatgcaaat tgtcgccacc
    10501 tgcaagatct accacatcct gactgccgat aaccgttccg ctgctgccac tgacttcaag
    10561 gccatcattg tatgatgtgc cgctctgcca agtggagaaa tcagagagat cgagcacacc
    10621 gccgtttaac acgatcgact gcggatcgtc ctggagagcg gtaagatctg ccgcgtcgcc
    10681 ttccagcgaa agcaccgcac catcatcaac gactatatcg ccggttagcg ccatcgactg
    10741 cgcccctgcc agcacgtagc tgccgttttg cgcaatggtg agctgaccag caccttcgat
    10801 gatgccagca aaactgccct gattaacagt aacgttgcca ccagcatcga tatttaaagt
    10861 gccattctga aagcccgtca atgcgtgcac aaaagtttgt tgggtcgagc caacgtttag
    10921 ctcagcctga ttctgatact gatcaatact ccctatcgtc agaccgtagc agtcttgcgg
    10981 atcgtcctgg caatgcgtat cgccgacatt catcagggag ttgctgcgac ccagggtaac
    11041 ttcaccgttt tcaatctgca tctcaccagt aaagtcattg ttatctgcat taagtaccag
    11101 atcgccggaa cctgttttgg tgattaaccc ggtaccagca atagagtcaa cagctccgtc
    11161 attctctgta ttgccgataa ccagcgtttt tccgtcggca atatcaaagg taacttcact
    11221 taagccgaga tacataaagc cacccgccgc agtggaggga ccatctccat agcctgctgc
    11281 gctattgttc tcatcgacta acacgccgcc gttctggctg tagctgtcat caacagaaat
    11341 atcaataaga tagggagccg tcgcgctatt ggtatatatc gccccgccat aaccttcggc
    11401 tgtgttattt gtaaaggcgg tgttatttat tatcgtataa cctgaaggat gcttgctgtc
    11461 gctattatta tcggtaacat cgattgcccc gccatcgcca tcactgtaac ttgttgatgt
    11521 atatgcctgg ttattgttaa aaacatcatc acttaaatag acgtcattat taatggtata
    11581 aattgcgcca cctttgccat cattagcgat attattgatg gcgttagtga cacgtaaatc
    11641 gacggcacca gtatcattag taccagaaga atagattgcg ccaccatagc cgcctgcgac
    11701 gttaccggaa aaaataacat cagtaagatt cagcgttgag ttttctttag caaatatggc
    11761 cccgccgtta ttatattctc ctgtaacggt gttattggca aacagagtca ttccagtttc
    11821 attttctggt aatagtgaaa attccgctcc ttgttgcaaa aatacggccc caccgctggc
    11881 attattggtc atatccgaaa aaaccagcca ttgcccatcg gcg
//
LOCUS       DAJTQY010000108        11715 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.108, whole genome shotgun sequence.
ACCESSION   DAJTQY010000108 DAJTQY010000000
VERSION     DAJTQY010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11715
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.108"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            127..996
                     /locus_tag="OO349_003297"
     CDS             127..996
                     /locus_tag="OO349_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HCR5868259.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQDNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(1199..1552)
                     /locus_tag="OO349_003298"
     CDS             complement(1199..1552)
                     /locus_tag="OO349_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HCR5868260.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1690..3336)
                     /gene="groL"
                     /locus_tag="OO349_003299"
     CDS             complement(1690..3336)
                     /gene="groL"
                     /locus_tag="OO349_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HCR5868261.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3380..3673)
                     /locus_tag="OO349_003300"
     CDS             complement(3380..3673)
                     /locus_tag="OO349_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HCR5868262.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3949..5205
                     /gene="yjeH"
                     /locus_tag="OO349_003301"
     CDS             3949..5205
                     /gene="yjeH"
                     /locus_tag="OO349_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HCR5868263.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTLGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPASIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLVGLWLFLPKRKTPENGITT"
     gene            complement(5221..5697)
                     /gene="fxsA"
                     /locus_tag="OO349_003302"
     CDS             complement(5221..5697)
                     /gene="fxsA"
                     /locus_tag="OO349_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HCR5868264.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            6034..7470
                     /gene="aspA"
                     /locus_tag="OO349_003303"
     CDS             6034..7470
                     /gene="aspA"
                     /locus_tag="OO349_003303"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HCR5868265.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7588..8889
                     /gene="dcuA"
                     /locus_tag="OO349_003304"
     CDS             7588..8889
                     /gene="dcuA"
                     /locus_tag="OO349_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HCR5868266.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            9005..9343
                     /gene="cutA"
                     /locus_tag="OO349_003305"
     CDS             9005..9343
                     /gene="cutA"
                     /locus_tag="OO349_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HCR5868267.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            9319..11016
                     /gene="dsbD"
                     /locus_tag="OO349_003306"
     CDS             9319..11016
                     /gene="dsbD"
                     /locus_tag="OO349_003306"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HCR5868268.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADEQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            11053..11628
                     /locus_tag="OO349_003307"
     CDS             11053..11628
                     /locus_tag="OO349_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR5868269.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
BASE COUNT         2813 a         3024 c         2882 g         2996 t
ORIGIN      
        1 tttctgttta tatagagtga gcacggctat ttatgttatt ttaacaacta ttactaccaa
       61 gcccatttat ctggcttata aattaaaatt aattatttaa ttgagaaatg gttagggaga
      121 acctacatgg ccataagcat caagggtgtt aataccggtg ttattcgcaa gagtaataat
      181 ttcattgcac tggcgctgaa aattaaagaa cctcgcaata aagaatcgtt gttctttatg
      241 tctgtcatgg aactgcgcga cctgctgatt gcgctggaaa gtcgtctgca tcaaaagcat
      301 aaactggatg ctgctgcacg ccttcagtat gagcaagctc gcgataaagt cattaagaaa
      361 atggcagaaa acatcccaga aattctggtt gatgagctta aaaatgccga tattaatcgt
      421 cgggttaaca cgctggaact taccgataat caaggcgaaa atcttacctt cgtattaacg
      481 ctacatgacg gtagcaaatg tgagttagtc gtcaatgagt tgcagattga aatgctggcg
      541 cgagcaatca ttcatgcaat taataatgcg gaaatgcgtg aactggcatt acgtattacc
      601 tcactgttag atttcctgcc gttatacgac gttgactgcc aggacaatgg caatctggaa
      661 tatgacacct attcccaacc ggaatggaaa cataacttgt tcgaccatta tctggcggtg
      721 ctttaccgct ttaaagatga aagtggcaaa gaacaattta gcggcgcagt ggtgaaaacg
      781 cgtgaagcaa cgccgggtaa agaaatagag gcgatcactc gccgaatgct tgatttcagc
      841 ccacgactga aaaaactcgc gggtgtgcca tgtcaggtct atgttcgcac cgtagcggcg
      901 aataacgccc aaccgctaac ccaggatcaa tgcttacgcg cgctgcatca cttacgtgta
      961 caatccacca gcaaaactgc accacaggcg aaataatctc atctttgcta atgcgaggct
     1021 ggagccatcc agcctgaaat gactgataac gtgatcgttg gtcatgccgg atgcggcgta
     1081 aacgccttat ccggcctaca aattctcaag cattcaatca gttgcaatgc ctgctgtagg
     1141 cctgataagc gaagcgcatc aggcaataat acgtttgtca cccagcctct ggatcacatc
     1201 agttcgggca cttataaacc tgaccgataa tctggctatc cgtcgggaca aaactggaca
     1261 acattccctg cgacgggctg ctgatgccat aaatcacgtt accgcccatt gcagccgcct
     1321 ggttgcgcag atcgtttgct gcgccgcgca tagaaccgcc ctcttctccg tgttgcccgg
     1381 aaagccagtt gctttgctta cctgtcgcag taccaatcag ctggcactct gcgcctggct
     1441 gctcgtccac aatgcgtaca ctctgaccgg cagcactcaa ttcgttgctg gagctacaac
     1501 ccgccatcag tagcgcggca ccgacaatcc ctgctaagta ttttacgtgc atgttattcc
     1561 ccataatcaa tgagctggac gccactcgcg tcatccgtgt ctgaatctta tactagaaag
     1621 atgaccaaaa gaaaaacccc cagacatttc tgcccggggg tttgtttatt tctgcgaagt
     1681 gcagggcaat tacatcatgc cgcccatgcc acccatgccg cccataccgc cagcagcgcc
     1741 taagtcagct gcatcgtttt tcggcaggtc ggtaaccatg cattcggtgg tgatcatcag
     1801 gccagccaca gaagctgcgt actgcagagc agaacgggtt actttggttg ggtccaggat
     1861 acccatgtcg atcatgttgc cgtattcttc ggttgctgcg ttgtaaccgt agttgccgtc
     1921 gccgccttta acggtgttag caacaacaga cggttcttcg ccgcagttca ggacgatctg
     1981 acgcagcgga gcttccattg cacgcagtgc aactttgata cccacgttct ggtcttcgtt
     2041 ctgaccacgc aggtcagcca gtttagacgc tacgcggatc agcgcaacac caccaccagc
     2101 aaccacgcct tcttctaccg cagcacgggt cgcgtgcagg gcatcttcaa cgcgtgcttt
     2161 tttctctttc atttcaactt cggtagcagc acctactttg ataactgcaa cgccgcctgc
     2221 cagtttcgct acgcgctcct gcagtttttc acggtcgtag tcagaagttg cttcttcaat
     2281 ctgctgacgg atctgagcaa cacggccctg gattgcagct tcttcaccca cgccatcgat
     2341 gatagtggtg gtgtctttgt tgatcacaac acgtttagcc tgacccaggt cttccagggt
     2401 tgctttttcc agctccatac cgatctcttc agagattacg gtaccgccag tcagggttgc
     2461 gatatcctgc agcatagctt tacgacgatc gccgaagccc ggagctttaa ccgcagcaac
     2521 tttcacgatg ccacgcatgg tgttaacaac cagagttgct agcgcttcgc cttcaacatc
     2581 ttcagcgatg atcagcagcg gtttgcctgc tttggcaaca gcttccagaa ccggcagcat
     2641 ttcgcggatg ttggagattt tcttgtcagc cagcaggatg aacgggcttt ccagttctac
     2701 tgcgccagtt tccggcttgt tgatgaagta aggagacagg tagccacggt cgaactgcat
     2761 accttcaacc acgtccagtt cgtcctgcag accggtaccg tcttcaacgg tgataacgcc
     2821 ttctttaccg actttgtcca tcgcttcagc gatcagttta cctacggttt cgtcggagtt
     2881 agcggagatg gtaccaacct gagcaatcgc tttagaatca gagcacggta cggacagcgc
     2941 tttcagttct tcaactgcag cggtaaccgc tttgtcgata ccacgtttca ggtccatcgg
     3001 gttcatgccc gcagcaacag ctttcagacc ttcagtgatg atagcctgag ccagtacggt
     3061 tgcagtggtg gtaccgtcgc ctgcagcgtc gttcgctttg gaggcaactt ctttcaccat
     3121 ctgcgcaccc atattttcga acttgtcttc cagttcgatt tcacgagcaa cggaaacacc
     3181 atctttggtg atggtcggtg caccgaaaga tttatccaga actacgttac ggccttttgg
     3241 accgagggta actttcactg catctgccag tacgtttacg ccgcgcagca ttttcacacg
     3301 agcgtcgtta ccgaatttta cgtctttagc tgccattatc tttattcctt aaattcgtat
     3361 gttcagtgtc gtgcgcggat tacgcttcaa caattgccag aatgtcgctt tcggacatga
     3421 tcaacacttc ttcattgtcg atcttctcgg atttcacacc gtagccatcg ttgaaaataa
     3481 cgatgtcgcc aactttcacg tccagcggct tcacttcgcc attttcaagg atacggccat
     3541 tgccgacagc cagcacttcg ccgcgggtgg atttagccgc tgcagagccg gtcagaacga
     3601 tgccgccagc agatttagtt tcaacttctt tacgcttgac gatcacgcga tcatgcaatg
     3661 gacgaatatt cattgataac tctcctttga gaaagtccgt atctgttatg ggtgacgccg
     3721 gagcttacgt ggtttcccgg ctggtgacca gagaaatggg gatgaggctt cgccccttca
     3781 agggggaaaa agaaaaaaaa ttctgatcag cacaaaatcg ggtgaaaacc ctgattcacc
     3841 tcacatttca tcgcaatttc ttcatcgccg tcataagcga atctgattgt gctaccatcg
     3901 aaaatctacg cagttgccca aaatttgggc gcaatcggat atcaacccat gagtggactc
     3961 aaacaagaac tggggctggc ccagggcatt ggcctgctat cgacgtcatt attaggcact
     4021 ggcgtgtttg ccgttcctgc gttagctgca ctggtagcag gcaataacag cctgtgggcg
     4081 tggcccgttt tgattatctt agtgttcccg attgcgattg tgtttgcgat tctgggtcgc
     4141 cactatccca gcgcaggcgg cgtcgcgcac ttcgtcggta tggcgtttgg ttcgcggctt
     4201 gagcgagtca ccggctggct gtttttatcg gtcattcccg tgggtttgcc tgccgcacta
     4261 caaattgccg ccggattcgg ccaagcaatg tttggctggc atagctggca actgttgttg
     4321 gcagaactcg gtacgctggc gttggtgtgg tatatcggta ctctcggtgc cagttccagt
     4381 gctaatctac aaaccgttat tgccggactt atcgtcgcac tgattgtcgc tatctggtgg
     4441 gcgggcgata tcaaacctgc gagtatcccc ttccccgcgc caggaaatat cgaacttacc
     4501 gggttatttg ctgcgttatc agtgatgttc tggtgttttg tcggtctgga agcatttgcc
     4561 catcttgcct cggaatttaa aaatccagag cgtgattttc ctcgtgcttt gatgattggt
     4621 ctgctgctgg caggattagt ctactggggc tgtacggtag tcgtcttaca cttcgacgcc
     4681 tatggtgaac aaatggcggc ggcagcatcg cttccaaaaa ttgtagtaca gttgttcggt
     4741 gtaggagcgt tatggattgc ctgcgtaatt ggctatctgg cctgctttgc cagtcttaac
     4801 atttatatac agagcttcgc ccgcctggtc tggtcgcagg cgcaacataa tcctgaccac
     4861 tacctggcac gcctctcttc tcgccatatc ccgaataatg ccctcaatgc ggtgcttggc
     4921 tgctgcgtgg tgagcacgtt ggtaattcat gccttagaga tcaatctgga cgctcttatt
     4981 atttatgcca atggcatctt tattatgatt tatctgttat gcatgctggc aggctgtaaa
     5041 ttattgcaag gtcgttatcg actactggcg gtggttggcg ggctgttatg cgttctgtta
     5101 ctggcaatgg tcggctggaa aagtctctat gcgctgatca tgctggtggg gttatggctg
     5161 tttctgccaa aacgaaaaac gccggaaaat ggcataacca cataatccgg cgtttcgaca
     5221 ttaatcctgg cgatcgtctt tatgatcaag gcggtcgcgg tcatcatcct ttcgctgata
     5281 ctcaccatca aaagtattac cgccaccggt cccggcgcta aaaccgccgc ctggcatgcg
     5341 agaaaagcgc aaatgtggca tcaacttcac tgtcagatgc ttttgcaccg gcggcaataa
     5401 aagcagaaga ccgaggaagt cggtgaaaaa gcccggtaat aaaagcagca aaccggcaat
     5461 aatcagcgaa acacttttaa tcatctccgc cgcagggttt tcacccgccg ccattttttg
     5521 ctgcatcagc acgaaattct taaagccctg gttacgtacc agtgacatac cgataactga
     5581 cgtgaatata accagcacga gggtcagcaa tacccccaat acatgggcaa cctgaataaa
     5641 gattgaaatc tcaatataga cataaaggaa aatggcaata aaaggtaacc agcgcaaagg
     5701 tttctcctgt aatagcagcc ggttaaaccc ggctacctga atgggttgcg aatcgccttc
     5761 agcttatatt gtggtcatta gcaaaatttc aagatgtttg cgcaactatt tttggtagta
     5821 atcccaaagc ggtgatctat ttcacaaatt aataattaag gggtaaaaac cgacacttaa
     5881 agtgatccag attacggtag aaatcctcaa gcagcatatg atctcgggta ttcggtcgat
     5941 gcaggggata atcgtcggtc gaaaaacatt cgaaaccaca tatattctgt gtgtttaaag
     6001 caaatcattg gcagcttgaa aaagaaggtt cacatgtcaa acaacattcg tatcgaagaa
     6061 gatctgttgg gtaccaggga agttccagct gatgcctact atggtgttca cactctgaga
     6121 gcgattgaaa acttctatat cagcaacaac aaaatcagtg atattcctga atttgttcgc
     6181 ggtatggtaa tggttaaaaa agccgcagcc atggcaaaca aagagctgca aaccattcct
     6241 aaaagtgtag cgaatgccat cattgccgca tgtgatgaag tcctgaacaa cggaaaatgc
     6301 atggatcagt tcccggtaga cgtctaccag ggcggcgcag gtacttccgt aaacatgaac
     6361 accaacgaag tgctggccaa tatcggtctg gaactgatgg gtcaccagaa aggtgaatat
     6421 cagtacctga acccgaacga ccatgttaac aaatgtcagt ccactaacga cgcctacccg
     6481 accggtttcc gtatcgcagt ttactcttcc ctgattaagc tggtagatgc gattaaccaa
     6541 ctgcgtgaag gctttgaacg taaagctgtc gaattccagg acatcctgaa aatgggtcgt
     6601 acccagctgc aggacgcagt accgatgacc ctcggtcagg aattccgcgc tttcagcatc
     6661 ctgctgaaag aagaagtgaa aaacatccaa cgtaccgctg aactgctgct ggaagttaac
     6721 cttggcgcaa cagcaatcgg tactggtctg aacacgccga aagagtactc tccgctggca
     6781 gtgaaaaaac tggctgaagt cactggcttc ccatgcgtac cggctgaaga cctgatcgaa
     6841 gcgacctctg actgcggcgc ttatgttatg gttcacggcg cgctgaaacg cctggctgtg
     6901 aagatgtcca aaatctgtaa cgacctgcgc ttgctctctt ctggtccacg tgccggcctg
     6961 aacgagatca acctgccgga actgcaggcg ggctcttcca tcatgccagc taaagtaaac
     7021 ccggttgttc cggaagtggt taaccaggta tgcttcaaag tcatcggtaa cgacaccact
     7081 gttaccatgg cagcagaagc aggtcagctg cagttgaacg ttatggaacc ggtcattggc
     7141 caggccatgt tcgaatccgt tcacattctg accaacgctt gctacaacct gctggaaaaa
     7201 tgcattaacg gcatcactgc taacaaagaa gtgtgcgaag gttacgttta caactctatc
     7261 ggtatcgtta cttacctgaa cccgttcatc ggtcaccaca acggtgacat cgtgggtaaa
     7321 atctgtgccg aaaccggtaa gagtgtacgt gaagtcgttc tggaacgcgg tctgttgact
     7381 gaagcggaac ttgacgatat tttctccgta cagaatctga tgcacccggc ttacaaagca
     7441 aaacgctata ctgatgaaag cgaacagtaa tcgtacaggg tagtacaaat aaaaaaggca
     7501 cgtcagatga cgtgcctttt ttcttgtgag cagtaactta aaaataacaa tctaatatca
     7561 acttgttaaa aaacaaggaa ggctaatatg ctagttgtag agctcatcat agttttgctg
     7621 gcgatcttct tgggcgccag attgggggga ataggtattg gttttgcagg cggattgggg
     7681 gtgctggttc ttgccgctat tggcgttaaa cccggtaaca tcccgttcga tgtcatttcc
     7741 attatcatgg cggttatcgc cgctatttct gccatgcagg ttgctggcgg tctggactat
     7801 ctggttcatc agacagaaaa gctgctgcgc cgtaacccga aatacatcac gatcctcgca
     7861 ccgatcgtga cctatttcct gactatcttt gctggtactg gcaacatctc tctggcgaca
     7921 ctgccagtta tcgctgaagt tgcgaaggaa caaggcgtta aaccttgccg tccgctctct
     7981 actgcagtgg tatccgcgca gattgcgatc accgcatcgc caatctcagc ggcagtggtt
     8041 tacatgtctt ccgtgatgga aggtcatggc atcagctacc tccatctgct ctccgtggtc
     8101 atcccgtcca ccctgctggc ggttctggtg atgtccttcc tggtcactat gctgttcaac
     8161 tccaaactct ctgacgatcc gatttatcgc aagcgtctgg aagagggctt ggttgaactg
     8221 cgcggtgaaa agcagattga aatcaaatcc ggtgcaaaaa cgtccgtctg gctgttcctg
     8281 ctgggcgtag ttggcgtggt tatctatgca atcatcaaca gcccaagcat gggtctggtt
     8341 gaaaaaccgc tgatgaacac caccaacgca atcctgatca tcatgctcag cgttgcaact
     8401 ctgaccaccg ttatctgtaa agtcgatacc gacaacatcc tcaactccag caccttcaaa
     8461 gcaggtatga gcgcctgtat ttgtatcctg ggtgttgcgt ggctgggcga tactttcgtt
     8521 tccaacaaca tcgactggat caaagatacc gctggtgaag tgattcaggg tcatccgtgg
     8581 ctgctggccg tcatcttctt ctttgcttct gctctgctgt actctcaggc tgcaaccgca
     8641 aaagcactga tgccgatggc tctggcactg aacgtttcac cgctgaccgc tgttgcttct
     8701 ttcgctgcgg tgtctggtct gttcattctg ccgacctacc cgacgctggt tgctgcggta
     8761 cagatggatg acacgggtac tacccgtatc ggtaaattcg tcttcaacca tccgttcttc
     8821 atcccgggta ctctgggtgt tgccctggcc gtttgcttcg gcttcgtgct gggtagcttc
     8881 atgctgtaat gatcaatcgc ggggcgttca cgccccgctt tctttcccgc cgactaacat
     8941 ccttcccccg tccgttgtat agtgacctct ctcttgcggt tccatctgtt cttgcgaggt
     9001 gtttatgctt gatgaaaaaa gttcgaatac cgcgtctgtc gtggtgctat gtaccgcacc
     9061 ggatgaagcg acagcccagg atttagccgc caaagtgctg gcggaaaaac tggcggcctg
     9121 cgcgaccttg atccccggcg ctacctctct ctattactgg gaaggtaagc tggagcaaga
     9181 atacgaagtg cagatgattt taaaaactac cgtatctcac cagcaggcac tgctggaatg
     9241 cctgaagtct catcatccat atcaaacccc ggaacttctg gttttacctg ttacacacgg
     9301 agacacagat tacctctcat ggctcaacgc atctttacgc tgattctgct actttgcagc
     9361 acttccgttt ttgccggatt attcgacgcg ccgggacgtt cacaatttgt ccccgcggat
     9421 caagcctttg cttttgattt tcagcaaaac caacatgacc ttaatctgac ctggcagatc
     9481 aaagacggtt actacctcta ccgtaaacag atccgcatta cgccggaaca cgcgaaaatt
     9541 gccgacgagc agctgccgca aggcgtctgg catgaagatg agttttacgg caaaagcgag
     9601 atttaccgcg atcggctgac gcttcccgta accatcaacc aggcgagtgc gggagcgacg
     9661 ttaactgtca cctaccaggg ctgtgctgat gccggtttct gttatccgcc agaaaccaaa
     9721 accgttccgt taagcgaagt ggtcgccaac aacgcagcgt cacagcctgt gtctgtttcg
     9781 cagcaagagc agcacaccgc gcaattgccc ttttccgcgc tctgggcgtt gttgatcggt
     9841 attggtatcg cctttacgcc atgcgtgctg ccaatgtacc cactgatttc tggcatcgtg
     9901 ctgggtggta aacagcggct ctccactgcc agagcattgt tgctgacctt tatttatgtg
     9961 caggggatgg cactgactta cacggcgctg ggtctggtag ttgccgccgc agggttacag
    10021 ttccaggcgg cgctacagca cccatacgtg ctcattggcc tcgccatcgt ctttaccttg
    10081 ctggcgatgt caatgtttgg cttgtttact ctacaactcc cctcttcgct gcaaacacgt
    10141 ctcacgttga tgagcaatcg ccaacagggc ggctcacctg gcggtgtgtt tgttatgggg
    10201 gcgattgccg gactgatctg ttcaccatgc accaccgcac cgcttagcgc gattctgctg
    10261 tatatcgccc aaagcgggaa catgtggctg ggcggcggca cgctttatct ctatgcgttg
    10321 ggcatgggcc tgccgctgat gctaattacc gtctttggta accgcttgct gccgaaaagc
    10381 ggcccgtgga tggaacaagt caaaaccgcg tttggttttg tgatcctcgc actgccggtc
    10441 ttcctgctgg agcgagtgat tggtgatgta tggggattac gcttgtggtc ggcgctgggt
    10501 gtcgcattct ttggctgggc ctttatcacc agcctacagg ctaaacgcgg ctggatgcgc
    10561 gtggtgcaaa taatcctgct ggcagcggca ttggttagcg tgcgcccact tcaggattgg
    10621 gcatttggtg cgacgcatac cgcgcaaact caaacgcatc tcaactttac acaaatcaaa
    10681 acggtagatg agttaaatca ggcgctcgtt gaagccaaag gcaaaccggt gatgttagat
    10741 ctttatgccg actggtgcgt cgcctgtaaa gagtttgaga aatacacctt cagcgacccg
    10801 caggtgcaaa aagcgttagc agacacggtc ttacttcagg ccaacgtcac ggccaacgac
    10861 gcacaagatg tggcgctgtt aaagcatctt aatgtccttg gcctaccgac aattctcttt
    10921 tttgacggac aaggccagga gcatccacaa gcacgcgtca cgggctttat ggatgctgaa
    10981 accttcagcg cacatttgcg cgatcgccaa ccgtgaacga cacttgcagt gggaaagacg
    11041 gaggaaaata gcgtgcaacg tgaagatgta ctgggagaag ccctgaaatt attagaatta
    11101 caagggattg ccaacaccac gctggagatg gttgctgaac gtgtggatta tccactggac
    11161 gagctacgcc gcttctggcc agacaaagag gcaatcctct acgatgcgct gcgttatctt
    11221 agccaacaga tagatgtctg gcgtcgtcag ctgatgctgg acgaaacgca aaccgccgaa
    11281 caaaagctgc tggcacgtta tcaggcgtta tcggagtgcg ttaaaaacaa ccgctatccg
    11341 ggctgtctat ttatcgctgc ctgtacgttt tatcccgatc ctggccaccc tattcatcaa
    11401 ctggccgatc agcaaaaaag cgctgcctac gatttcaccc acgaactgtt aaccacgctg
    11461 gaagttgacg atccggcgat ggtagcaaag cagatggaac tggtgctgga aggctgttta
    11521 agccgaatgc tggtgaatcg tagccaggcg gatgtcgaca ctgcacatcg gctggcggaa
    11581 gatatcctgc gcttcgcccg ctgccgccag ggtggtgcat tgacctgaca gaaacacaga
    11641 aaagaagcga tttgccgcaa tcttaagcag ttgaatcgct tttactgaaa ttaggttgac
    11701 gaaatgtgca gatta
//
LOCUS       DAJTQY010000109        11691 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.109, whole genome shotgun sequence.
ACCESSION   DAJTQY010000109 DAJTQY010000000
VERSION     DAJTQY010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11691
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.109"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            360..419
                     /gene="ibsE"
                     /locus_tag="OO349_003308"
     CDS             360..419
                     /gene="ibsE"
                     /locus_tag="OO349_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HCR5868270.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(539..1972)
                     /gene="hldE"
                     /locus_tag="OO349_003309"
     CDS             complement(539..1972)
                     /gene="hldE"
                     /locus_tag="OO349_003309"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HCR5868271.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVFYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(2020..4860)
                     /gene="glnE"
                     /locus_tag="OO349_003310"
     CDS             complement(2020..4860)
                     /gene="glnE"
                     /locus_tag="OO349_003310"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HCR5868272.1"
                     /translation="MKPLSSPLQQYWQTVVERLPELLAEESLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQPPQADEWQHYSVWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPLLQSRSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWGQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRNRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERDLVRASWQKWLVEE"
     gene            complement(4883..6184)
                     /gene="ygiF"
                     /locus_tag="OO349_003311"
     CDS             complement(4883..6184)
                     /gene="ygiF"
                     /locus_tag="OO349_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HCR5868273.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRRHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEI
                     WPNGELPADLASRVQSLFSTDFYREKWLVEINGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELVLAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLMRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            6426..7046
                     /locus_tag="OO349_003312"
     CDS             6426..7046
                     /locus_tag="OO349_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HCR5868274.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            7110..8348
                     /gene="cca"
                     /locus_tag="OO349_003313"
     CDS             7110..8348
                     /gene="cca"
                     /locus_tag="OO349_003313"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HCR5868275.1"
                     /translation="MKIYLVGGAVRDALLGLSVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIRTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREGWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(8511..9332)
                     /gene="bacA"
                     /locus_tag="OO349_003314"
     CDS             complement(8511..9332)
                     /gene="bacA"
                     /locus_tag="OO349_003314"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCR5868276.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(9423..9791)
                     /gene="folB"
                     /locus_tag="OO349_003315"
     CDS             complement(9423..9791)
                     /gene="folB"
                     /locus_tag="OO349_003315"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HCR5868277.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            9896..10513
                     /gene="plsY"
                     /locus_tag="OO349_003316"
     CDS             9896..10513
                     /gene="plsY"
                     /locus_tag="OO349_003316"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HCR5868278.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPISWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(10526..11457)
                     /gene="ttdR"
                     /locus_tag="OO349_003317"
                     /gene_synonym="dan"
                     /pseudo
     CDS             complement(10526..11457)
                     /gene="ttdR"
                     /locus_tag="OO349_003317"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
BASE COUNT         2756 a         3335 c         2887 g         2713 t
ORIGIN      
        1 catactgatt gtgttgttac tcgtaagttt cgcagcttat taacagccaa tcagaggggg
       61 gagaaatcct ccctcaccct tgttccttta ctttaggttg aaaaaaaatc agcgtcaaga
      121 aggtttcatt aacacatatc cctggcccaa aaagccgggg attttttata tctgcgttcc
      181 gctaaaaggt gcaaatgctc aggacgttgc agcgttttgc gtgaccgctc ggggaaggca
      241 aaactgcttc agggaaagcg ttgcgcgggg tccggcgctc atcaacaatc ggggagtcag
      301 caaggggctg aaacgggaaa gcccctcccg aggaaggggc cttatagaag gaaagggtta
      361 tgatgaagtt cgtcatcata ctggttgtgt tgttactgtt aagtttcccg acttactaac
      421 aactgatcag aggggggaga aatcctccct tacccttgtt cctttacttt aggttgaaaa
      481 aacaacagcg tcaataggcc tgcctgctac gaagcgagat ctgtgaaccg ctttccggtt
      541 agcctttttt atcctgttgg atcttcttga tgatgttagt ggtcgagcaa ccgtcttcga
      601 agttgagcac cagcacttcg ccgccgttgg cccagacttc tttactcccg gcgatctctt
      661 ctggtttata gtcgccgccc ttcaccagta aatctggcag gatcccggcg atcaagcgct
      721 gcggcgtgtc ttcttcaaac gacaccaccc agtcgaccgc ttccagtgcg cccagcacaa
      781 tcatacgctg ttcgagcggg ttaacggggc gagaatcccc tttcagccgt ttggtggagg
      841 catcgctgtt cacagcgaca atcaagcggt cacccagttt gcgggcattt gccagataga
      901 agacgtgtcc ggcgtgcagg atgtcaaaga cgccattggt catcaccact ttttcaccgc
      961 gtttacgcgc tgccgctacg gccagcttca gttcctcttc ggtcatcacg ccaaagccgg
     1021 tatctgcgcg cccacgcact gcattttcca gctcgatcgg cgaaaccgtg gaggttccca
     1081 gtttgccgac caccacgcca gccgccgcat tggcaaagaa gcaggcttct tccagcgaat
     1141 tacccgctgc cagcgttgcc gccaggacgc caatcaccgt gtcgcccgca ccggtaacgt
     1201 catacacttc ctgcgcctgg gttggcatat gcagcggcgc tttacccggt tgcagcagcg
     1261 acataccctg ttcggaacgg gtcactaaca gagccgagag ttcgtaatcg gcaatcagtt
     1321 tcatgccgcg ctcaacaatc tcttcttcgg tcttacattt accgacaaca gcttcaaatt
     1381 ccgagagatt cggtgtcagc agcgtagcgc cgcggtagcg ctcaaaatcg gtaccttttg
     1441 gatcaatcag caccggaacg cccgctttac gcgccagttg gatcatctgc tgtacgcttg
     1501 ccagcgcacc tttggcgtag tcagaaagca ccagcgcgcc aatcgaactc agcgcctgat
     1561 taatccgctc gtgcagcggc tgcggatcaa caccttcgaa accttcttca aaatccagac
     1621 ggatcagctg ttggttgcgg gaaagtaccc gtaatttggt gatggtcgga tgcgtcggta
     1681 cagaaacgaa gtcgcatttg acgttgacgt cggccagaga tttactcagc gcgcgcgctg
     1741 catcgtcaat gcccgtcaac ccgaccaggc gtgcattagc accgagagaa gcgatattca
     1801 tcgccacgtt agccgcgccg cccggacgtt cttcgatggt attcacttta accacgggca
     1861 ccggcgcttc cggcgagata cgactggtgg ggccgtacca gtaacgatcc agcatcacat
     1921 caccaaccac catcactcct gcacgttcaa actctggcag cgttactttc attcctgtct
     1981 cctgagagat tcaaaatttg cgcgcgataa taccatactt cattcttcca ccagccactt
     2041 ctgccagctt gcccgcacca gatcacgctc tgcggtgaag caatcctccg acacatggcc
     2101 cggcaattcc tgtaatgcca gatgatgaag ttcatcgcgc agcgtcgtgt aagcacgggt
     2161 cagcgccatc gcttcctgct cttccataat gtcgttttgc gccagcagct ccagaatacg
     2221 cacgttgtct gaccagcgcg ttaacttcgg tttttcatga gcgtagcgca acaccagata
     2281 ttgggtaata aattcgatat cggtaattcc gccttcatcg gctttgatat caaagcgatt
     2341 acgatgttta ttgccgagat gagcgcgcat tttctcgcgc atttcccgca cttccgtttg
     2401 cagagtttta ccttctcgcg gcagtgtcat aatctcacga cgcaccgcgt caaagtgcgc
     2461 agtgagctgc ggatcgccgt acactacacg cgcacgcacc agcgcctgat gttcccacgt
     2521 ccaggcctcg tttttctgat aatcggcaaa tgcttctgcg gatgtcacca gcattcccgc
     2581 cgccccggac ggacgcagtc gagcatccac ttcatacaaa atgccggaag aggtacgcgt
     2641 actgaacaga tgcataatgc gttgcgccag acgcagataa aactgccgcc cgtcgatttc
     2701 ccgctcgccg tcagtcatcg catccattgg gcaatcatgg aggaagataa ggtcaagatc
     2761 ggaactgtag cctaactccc agccgcccag cttgccgtag ccgaccaccg caaaaccgcg
     2821 cccttcacgt tcgttgaggt gatttggctt accgtagcgg gccaccattt gaccccacgc
     2881 ctgctgaacg acggcatcta tcatagcttc tgccagccag gttaagtgat cgctcacttt
     2941 cattaccggt agcgtaccgg cgatatccgc tgccgcaatg cgtaacagct gcgcctgttt
     3001 gaactgacgc agcgcctcaa gctgttgttc ttcgtcatct tccggcacgc gcagcaaata
     3061 ctggcgcagt tcatcacggt aggcatcggt cgccgtcggc tgataaaggg tatttggatc
     3121 gagcaattca tccagcaata atggataacg cgctagctgg ctggcaatca tcggcgatgc
     3181 ggcacacaga gaaatcaaat gtttaagcgc cgcagggaat tcactgagca attctaaata
     3241 ggttgtgcgg gtaacaatcc ccaccagcaa ggcggtaatg cgcgacagcg tgacagcagc
     3301 gtcttcacgc gcacagacat cacttagcag atgtggcatc agatggtcga gcacctgacg
     3361 tcctcgcggc ccgatagtac gcttatccag ctctttgcgg aaatcagcaa tcagcgttag
     3421 gacctgtttg cgatcatcct cgctaagatg cgccagcact ggcgtagtgt catcttcctg
     3481 caacgcatcc tgccacagct cacgccactg ttccgacagc gactcttcct gagtttcact
     3541 ttcgtcgtcg ccaatcaatt cattaaacac ccggcgcaca ttggtcatat gtgcggtcag
     3601 cgccccggtc agttgcggcc agtcagcaaa gtccatcgcc cacgccagcc gcgcacggtt
     3661 aagctcatca gaaggaagcg tttgggtttg ttcgtcgtta atgctttgca gcaggttttc
     3721 cagacgccgc aggaacagat atgccactcg caattgttca gcatcgtttt cagaaagcag
     3781 atgcagcgcg gcaatggcgc tgagcgttgg cagtaaagag cgcgactgca gcaacggttc
     3841 gcgcccaccg cgaatgagct ggaacacctg aacgataaat tcaatttcgc gaatgccgcc
     3901 tgcgccgagt ttgatattgt cggtcaaacc acgtcgacgc acttcacggg caatcatccc
     3961 tttcatgttg cgcagcgact gaatcacgct gaaatcgatg taacgacgga aaacaaacgg
     4021 gcgcagcatc gcacgcaact cgttagcata gacgccttcg ctatcgccca taatccgcgc
     4081 tttgaccatc gcgtaacgct cccagtcgcg tccctgctcc tggtaataat cctccaacgc
     4141 ggcaaagctc agcaccagcg ggccactttc gccaaacgga cgcagtcgca tatccacgcg
     4201 atagacgaag ccatcctgcg ttggttgatc cagcacttta atcagccgct gccccatgcg
     4261 ggtaaaaaac tgcgcgttat ccagttcccg gcgtccgccc tgcgtacaac catgttccgg
     4321 ccaggcaaaa atcagatcga tatcagagga gaaattcagt tccccgccgc ccagcttacc
     4381 catgcctaag atcagcagcg gttgcgcttc gccctgcgca ttgcacggcg ttccccactc
     4441 gcggcagcag gcgtcataca gccagtcacg cgccgcaaca atcagcgtct ccgccagata
     4501 actgagctgc tgcaatatgc tctcttcagt aaccagtgcc agcgtttgcg cccatgcgat
     4561 gcgcaccata atgcgccgcc ggaatagccg cagctcgcgc attaacccgg cttcgtcgct
     4621 cacattactg agcgcctcct gcaaccacac gctgtaatgt tgccattcgt cagcctgtgg
     4681 cggttggctt tccagttccg tcagccactc tggatgcgca atcacgctgt cctgcacaaa
     4741 atcactgaaa gtaagtactg acttcgcctg tgcactaagt gattcctcgg ctaaaagctc
     4801 tggcagccgc tcaacaacgg tctgccagta ctgctgtaac ggtgaagaga gcggcttcat
     4861 aagcgatttt atccttgcct gattaacgtt ttccactgtg caaccagaac ggttcctgat
     4921 tgtttgcctc attacggaaa tgttcaattt caatgcgttg cccggtcgca atagcgtgac
     4981 gcagcccctg ccagttctcc agccaggctt gcgcgacgac aggatcatag taacccgcca
     5041 gcaacagtat tgagtcaata tcacgcatca ggcgtggcaa ctggtcacgg tagcgatcgc
     5101 ctaacggctg gcagaaaacg cttttcagtt cagcggcatg gcgggaaaga tggatatcgg
     5161 caaagcgttt gaaggagtcg ctaattttgc cctgcgcttt ggcatctaaa aacggctgcc
     5221 atgctttgct taccagccat tcggtcaacg ccagcttcgc cattgccgtt tcggtagagt
     5281 agaccgccgt cacggcagaa accgcagaag cgatggtcgc ttcgcattga gtcagcaaat
     5341 cacgtaagtg agtactcgct ttacgcggca caatgccacc gaacagcatc agtgtatgac
     5401 ggactaggct aatggctgcc agcacctgct ctttcgccgc atcgttgccg cgtacccaca
     5461 gttcttcatg atactgccat tgcgctaaca ccagctcgag cgccgcttcc agcccctgtt
     5521 ccacatcggc ttttgccgca acatgcaaaa tggtggtcgg tttgatttca cgcgccggat
     5581 tgccctgcgc cagatgatag ccacgcgccg ctttgctcag gctgccctgg cgtaatccgg
     5641 tttgcgatac cagttggttc gccagtttca gcaccgcgcg cgtgtcgccg ctaagcagct
     5701 ccagttccag ctcgcagata ggctcagcaa attcacccgc tttcacttcc ccctggtcga
     5761 gagcgatttc aattcggctg ccattgatct ctactagcca tttttcgcga taaaaatcgg
     5821 tgctaaacag cgactgcacg cgggaagcga gatcggcagg caattcgccg ttcggccaga
     5881 tttccgtcgg taactgcgcc aggtcgagcg tcggttcgct caacgccacg ttatattccg
     5941 ggcgctggtg taagccgcct gtcactcttc ctgcaacttt catggtcatc tcatagcgac
     6001 cgttttcgcc acgaatacgt aagcccatat cgtgcctacg cagccagtta tccggcgttt
     6061 cgtagtaaat attcagcaac tgcacggggt catggtgctc gccgcccagc gtattgagat
     6121 ggtcacgcaa cgcctcaacg gcactgtgat taacaataaa ctttaattcg atttcctgag
     6181 ccatggcctt tacttatcgg ttatgtcaca taagtgacga tgaacggcga acttaatgcg
     6241 atcttttttg tcagtagata gtattttgcg ccaaattgcc atgcaacggg caatttgacg
     6301 ggcgtaaaag tttgaagcag tggcaaggcc tgacacagag gattccgatt gatgtcgaat
     6361 gctttgcgta gcgacactga tgccactact atcgttccac tttccatgac aataacgaca
     6421 gcctgatgcc aaaattacgc ctgatcggat taactttact cgcacttagc gcgactgccg
     6481 tctcacacgc tgaagaaacg cgctatgttt ctgacgaact gaatacctgg gtccgtagcg
     6541 gtccgggaga tcattatcgc ctcgtgggca cggttaacgc cggcgaggaa gtgaccttat
     6601 tacaaactga cgccaacacc aattatgccc aggtgaaaga cagctctggc cgtaccgcct
     6661 ggatcccgtt gaaacaactt agcactgagc caagcctgcg ctcccgtgtg ccagatctgg
     6721 aaaatcaggt caaaaccctg accgataaac tcaccaatat cgataacacc tggaatcagc
     6781 gcaccgcaga aatgcagcaa aaagtggcgc agagcgacag cgtgatcaac gggttaaaag
     6841 aagaaaatca gaaactgaaa aacgagctga ttgtcgcgca gaaaaaggtc gatgccgcca
     6901 gcgtacagct ggatgacaaa cagcgcacca tcatcatgca atggtttatg tatggcggtg
     6961 gcgtgctggg gcttggcttg ctgctcggtc tggtactgcc gcacctgatc ccaagccgca
     7021 aacgcaaaga tcgctggatg aactaaatcg ccttcttcgt tgcactgtct tattattagg
     7081 aattgtgtat ttgacgaagg gatgatggcg tgaagattta tctggtcggt ggtgctgttc
     7141 gggatgcatt gttaggacta tcggtcaaag acagagattg ggtggtggtc ggcagtacgc
     7201 cacaggagat gctcgacgcg ggctaccagc aggtaggccg cgattttcct gtgtttctgc
     7261 atccgcaaac gcatgaagag tatgcgctgg cacgtaccga acggaaatcc ggttccggtt
     7321 acaccggttt tacttgctat gccgcaccgg atgtcacgct ggaagatgat cttaagcgtc
     7381 gcgatctgac cattaatgcg ctggcccagg acgataacgg cgagattatc gacccgtaca
     7441 acggtctggg cgatctgcaa aatcgtctgt tgcgccatgt ttcccccgct tttggcgaag
     7501 atccgttacg cgtactgcgc gtggcgcgtt ttgctgcgcg ttatgcccac ctcggttttc
     7561 gtattgccga tgaaactctg acgctgatgc gcgagatgac ccatgcgggt gaactggaac
     7621 acctgacgcc tgaacgggta tggaaagaga cggaaagcgc cctcaccacc cgcaatcccc
     7681 aggtgttctt ccaggtactg cgcgattgcg gcgcacttcg cgttttattc ccggaaattg
     7741 acgcactgtt tggcgttccg gcacctgcca ggtggcatcc ggaaatcgac acgggtattc
     7801 ataccttaat gacgctctca atggcggcga tgctgagtcc gcaggttgat gtccgtttcg
     7861 caactttatg ccacgatctc ggtaaaggac tgacgccgcc agaactttgg ccgcgtcatc
     7921 atgggcatgg cccggcgggt gttaagttag tggaacaact atgccagcgt ctgcgcgtgc
     7981 caaatgaaat tcgcgattta gccagactgg tggctgagtt tcacgatctc atccgcacct
     8041 tcccaatgct gaacccaaaa accatcgtca aattatttga ttccatcgat gcctggcgta
     8101 aaccgcagcg tgtcgagcaa ctggcgctga ccagcgaggc tgacgtgcgc ggcagaaccg
     8161 gttttgaatc agcggactac ccgcaaggcc gttggttgcg cgaaggctgg gaagtggcgc
     8221 agtcagtgcc gacaaaagcg gtcgttgaag cgggatttaa aggcgtggag attcgcgagg
     8281 agctgacccg acggcggatt gcggcggtag ccagctggaa ggaacaacgt tgcccaaagc
     8341 ctgactgagt ataaaactaa acgttagcac gaaccgtccc ctcgcccctt cggggagagg
     8401 gttagggtga ggggaacaga ccagcaccgg tgcgaacaat cacccccacc ccggccctct
     8461 ccctacagag cgagggagaa aacccggcct gctcccgaac cttttctcaa tcaaaagaac
     8521 acgacataca ccgcagccgc cacaataaag cgataaatgg cgaacgggat aaacgaaatg
     8581 cgcttaatca attgcaggaa ggttttaatc gctatcagcg ccaccacaaa agcggtgata
     8641 aacccaacgg caaacatcgg gatatcgccg gttgtcagga agccccagct tttgtagaga
     8701 tcgagcgccg ttgcgcccat catcatcggt accgccagca ggaacgagaa ctcggaagca
     8761 gcgtaacggc tcacgcccat cagcatccca cctgaaatgg tcgccccgga acgggagaaa
     8821 cccggccaca gcgccagaca ctggaaacag ccaatcataa acgcctgacg ataggtcata
     8881 tcatcaagac ccggcgcacg cggctctttc ggcttcaggc attcggcggc aatcagcaac
     8941 aaaccgccaa cgaccagcgc atacatcaca tttatcgggt taaacaatga cttaatcgtg
     9001 tcgtggaata atagccccag taccaccgcc ggaatcatcc ccagcaaaat gtggatcagc
     9061 gttaaacgac ctttgctttc accttcgtgc tgcaacgggc ggccaaagtg gatgccaatc
     9121 aggccaaaca gacgccgcca gaacatcact actaccgcca gaattgatcc taactggatc
     9181 acaacttcaa aggttttcgc cgtgtcgccc tcaaacccca acaagtgacc gacaataatc
     9241 atatggcccg tgctggatac cggcagaaat tctgtcaatc cttcgaccac acccaatatt
     9301 gccgctatca gcagcgagtg catatcgctc atcaataaac ccctaaatta ttaaaatgta
     9361 ccgcttgtcc gaactactgc agtatgacca ggttataacc gtttggttta acagctgtaa
     9421 aattaattat tttctttcag attattgcca cgctcaatga ttacgccaac attcgccgcc
     9481 cgcgccactg cgcctggctt gctgagtttg atacgcaccc acggcgagtt gaagcgtgct
     9541 aacagcagct ccgccacctc ttcagccacg cgttccacca gcgcaaaacg cgccccctcg
     9601 acgtggctga ccaccgtttc tgcaatgtca gcgtaactga ggcaatccgc cacatcatca
     9661 cttttcgccg ctttacggtt atcccatgcc atttcgatat cgaacactaa cttctgttcg
     9721 atggtctgtt cccagtcgta aacaccaata gtggtgatta ccgaaagttg ctctataaat
     9781 acaatatcca tcacgtcctg cctgcttttt ggctaaccgg ataccacttc cggcgaaatg
     9841 tgcgtattat ccacagattc atcgttgaac acgaattttc aaaacggaac agcttatgag
     9901 tgcaatcgcg cctggaatga tcctcatcgc gtacctctgc ggctccattt ccagtgccat
     9961 tctggtttgc cgcttgtgtg ggctgcccga tccgcgaacc agcggctccg gcaatccagg
    10021 cgcaaccaat gtgttacgta tcggtggcaa gggagcagcc gtagcagtac tgattttcga
    10081 cgttctgaaa ggaatgttgc ctgtctgggg cgcgtatgaa ttaggtgtca gccccttctg
    10141 gctaggctta attgccatcg ccgcctgtct tggacacatc tggcccgttt tcttcggttt
    10201 taaaggagga aaaggcgttg ctaccgcttt tggagcaatc gcaccaatta gctgggatct
    10261 caccggagta atggcgggaa cctggttact gaccgtgcta ttgagcggat actcgtcgct
    10321 gggagcgatt gtcagtgcac tgattgctcc gttttatgtc tggtggttta agccacaatt
    10381 caccttcccg gtttcgatgc tctcttgcct gatcctgctg cgtcatcatg acaacatcca
    10441 gcgtctgtgg cgtcgtcagg agacaaaaat ctggacgaaa ttcaaaagaa agcgcgaaaa
    10501 ggatcccgag tgatttctgg tggatctaca tgacctgata gccttcatcg ggcttgccca
    10561 gccgttgctg gcaccatgcc gccagaaatt ccgcgcagac acgaatttca tgctgcgata
    10621 gagcagctcc cggtaaacag cccagatatt ggcgctttgt gcatactccg gcaatacttg
    10681 caccagtttg ccactctcca gaaacggcaa cacatcccac tcggaacgca gcataatccc
    10741 tttgccctcc agcgcccatt gcaacactat ctcgccgcta ttggaggaaa gatgcccgct
    10801 aaccttcacc gattttttct cctgaccgtt ccccaactcc catattccgt gggtcatatc
    10861 gcgctctttg gtcaccagac agtcatgacg acttaattcc tgtaatgatt gtggctgcgg
    10921 atatttttgc agatactcag gtgctgcgca taatattctt ttatttttcg ttaacagatg
    10981 cgcaatataa taatcaggaa tttcgtcatt aatacgaata tccagatcaa tattatcctg
    11041 cactaaatca atttgccgat cgaacaattc aaaatgcacc tgtaactcag gataattgcg
    11101 cataagttcg gtaatggctg gcgcaatatg gctgcgccca aaaccaaaac tgcaaccaat
    11161 acgaatcatc ccctccgggc gcgttttgat ttgcgtgacg tcatcgacca gtcgctgata
    11221 ttgggtgagg atttccagcg catgttcgta acaacgttgg ccactttcag tcagcgccac
    11281 gccgcgagcg gagcggttca gcagcgttgt tgccagcgta ttttcaagga tttggatccg
    11341 cttggtgaca aatgcaggcg tttgcccaag cgtcgccgct gcagcactaa aactcccgct
    11401 gtggacgatt tctaccagta cctgcaagtc tttggctaaa ggccagctat tcagcatcgt
    11461 taattatccg cagttgtgat aagcgcagtg tatttcgcaa aacattgcca ttccctgctc
    11521 tggcgcaata ttcattctta ttttcgcgtt gattcacgaa ctgttaatta catattcacg
    11581 gtggcaaaaa atataaaacc acatttttag tgttagtttg tggcggtgaa tttttccaga
    11641 caaatacaaa aactggagtt gccatgatga gcgaaagtaa taagcaacag g
//
LOCUS       DAJTQY010000110        11669 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.110, whole genome shotgun sequence.
ACCESSION   DAJTQY010000110 DAJTQY010000000
VERSION     DAJTQY010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11669
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.110"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            597..794
                     /locus_tag="OO349_003318"
     CDS             597..794
                     /locus_tag="OO349_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5868279.1"
                     /translation="MEITPQQLVEFTIPDGRGQQARTLPDMLSSDAGPLSYPLIYSSK
                     VMIISLFDSTVMPDKYRHNGK"
     gene            complement(744..1937)
                     /locus_tag="OO349_003319"
     CDS             complement(744..1937)
                     /locus_tag="OO349_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5868280.1"
                     /translation="MSPGIEDTPQKPLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFATDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            2073..3797
                     /gene="iucA"
                     /locus_tag="OO349_003320"
     CDS             2073..3797
                     /gene="iucA"
                     /locus_tag="OO349_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HCR5868281.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            3798..4745
                     /gene="iucB"
                     /locus_tag="OO349_003321"
     CDS             3798..4745
                     /gene="iucB"
                     /locus_tag="OO349_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase"
                     /protein_id="HCR5868282.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            4745..6487
                     /gene="iucC"
                     /locus_tag="OO349_003322"
     CDS             4745..6487
                     /gene="iucC"
                     /locus_tag="OO349_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucC"
                     /protein_id="HCR5868283.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            6484..7821
                     /gene="iucD"
                     /locus_tag="OO349_003323"
     CDS             6484..7821
                     /gene="iucD"
                     /locus_tag="OO349_003323"
                     /EC_number="1.14.13.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase"
                     /protein_id="HCR5868284.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFAHNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAASLTHYTANIQE"
     gene            7827..10022
                     /gene="iutA"
                     /locus_tag="OO349_003324"
     CDS             7827..10022
                     /gene="iutA"
                     /locus_tag="OO349_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HCR5868285.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IAAGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFIGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            complement(10573..10716)
                     /locus_tag="OO349_003325"
     CDS             complement(10573..10716)
                     /locus_tag="OO349_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5868286.1"
                     /translation="MEHRSVIHDELAIDHDAILQCAEEVLHHCVIQTVSFVRHILCYS
                     MGL"
     gene            10966..>11669
                     /locus_tag="OO349_003326"
     CDS             10966..>11669
                     /locus_tag="OO349_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR5868287.1"
                     /translation="MNKIYSLKYSAATGGLIAVSELAKRVSGKTNRKLVATMLSLAVA
                     GTVNAANIDISNVWARDYLDLAQNKGIFQPGATDVTITLKNGDKFSFHNLSIPDFSGA
                     AASGAATAIRGSYSVTVAHNKKNPQAAETQVYAQSSYKVVDRRNSNDFEIQRLNKFVV
                     ETVGATPAETNPTTYSDALERYGIVTSDGSKKIIGFRAGSGGTSFINGESKISTNSAY
                     SHDLLSASLFEVTQWDS"
BASE COUNT         2961 a         2900 c         2993 g         2815 t
ORIGIN      
        1 ggagtgaaac aaaggcagct tatcgttgtt tttcaaacga taaagtctct gctaataaaa
       61 tcatgactcc tcacaaagaa aatatcactg aaagagctca gcagtttaaa agagttttag
      121 ttttacagga tacaacagaa cttaattatt ctggtcagaa agaaaaacaa ggagttggtc
      181 ccaaaaaaca taaagatgaa agaaacttat ttcttcatcc tcaactcgtc atcagtgagt
      241 caggtgtttg cctcggtgtt tatgatgact atcagtggtt cagggatgaa ttaaaaacca
      301 gtaaaaatac ccgacaagaa atctgcaatg atttacttca taaaaaacat gtcagtgaaa
      361 aagaaacctg gcgttgggtt gaaggatata ataaagcaac agaactggct cgccaatgcc
      421 ctgataccca tgtattgtca atttctgatc gtgaaggcga tttttatgac ctgtttgaac
      481 gcgcagcgca gactcccgga ataaaagctg actggcttgt cagaatgaaa tttaataaca
      541 gagcaacgct gaatattaat ggtaaacggg atcataggtt gttgcacgaa agaatcatgg
      601 agattacacc gcagcaactg gttgaattca ctatacctga tgggcggggg cagcaagcca
      661 gaacactgcc tgacatgctt tcatcagacg caggtcctct ctcatatcct ctgatttact
      721 ccagtaaggt gatgatcata tctttatttg atagtacggt aatgccagat aaatacagac
      781 acaatggtaa ataatatagt tgcaaataat gctgccataa tcagcgcgat tgcgacacct
      841 gttttcgata ccagcggcac cagcaaagcg cacagtccat agccaagtgt gtgactggta
      901 gcgagccagc ccgcgcctgc gccatcagcc agcttatcat tcagtagaag ttgataagcg
      961 ggtgttgcca gagctgctcc aaatgacagc actgcacagc ccgggtaaaa caaccatagc
     1021 tgttcggaga gcatgatagc cagaccacca ctcatcaaca ctccggcact caggagcagg
     1081 gccaccggag tcaggcgctg cggacggagt accccgaact gcgcgataag cgcagctact
     1141 gcggacagac ccaacagcca ggccacctgt tggctaatgg cggtggtatc cgtagcgaac
     1201 tggcgagtaa gggcaggcga aagtccaagc tgcatcatgc tcaccgccgc agccagtaat
     1261 attgcgcaaa gcagataagg cagacaatcc cgcttcagac tgacgctctt gcactccggt
     1321 gtgggctgtg gtggtgtgcc gggcaaccgc agcagcatca gcagcgccag cactggggct
     1381 gccatcagca gccccagtgg cgccagagga tgaatggcca gcattgctgc cgcgcacagc
     1441 ggcccgaata gcctgccgca actcaggccg gagctgatgg ttgccagagc ggccatgcga
     1501 ttcccttctc cagctctctg caatgcccag acctgacagg ctggcaccat tgccgacacg
     1561 gtcagcccgt aggcgatccg ggcgatgatt aatattccca gtcccaccgc tgttgttatg
     1621 gcgctggtag ccatcagcac gcttcccagc cctagcaggg taaagcttat gccatatcct
     1681 cccagagccc acaacaccac aggtttactg ccagtacggg aaatttgctt tccccaccac
     1741 ggggacgatg gcagaaacag cattgagcca agcatcagca gggcagccca tacagacaga
     1801 ctcagatttg tctgtatgac cagaacaggg agcacaacca gcagaccgtt ctgtccgata
     1861 ccgagaagcc cggcactgaa cgcaagtggc cagcaggaca gtggtttttg gggcgtatct
     1921 tcgatccctg gtgacattat aatgtttcaa ctccatgtat taattgtgtt tatttgtaaa
     1981 attaatttat ctgacaataa catttattgt tgataatgag aatcattatt gacataattg
     2041 ttattatttt actgtgtggg agctgtttga ctatgaccct gccctctgaa aaaccagcca
     2101 cagatgtggc tgcgcagtgc ttcctgaatg cactgattcg tgaaaccaca gactggaaac
     2161 tgacagaata cccgccagac gaattgctca tcccgctgga tgagcagaaa tcgctccatt
     2221 tcagagtggc ttatttctcc ccaacccagc atcaccgctt tgcatttcct gcacgtctgg
     2281 tcacggcatc aggcagttat cctgtcgact ttaccactct ctcccggctt attattgata
     2341 agctacgcca tcaactgttt ctgcccgttc ccctctgcga aactttccac cagcgcgtgc
     2401 tggaaagcca tgtccatacg caacaggcaa ttgatgcccg tcatgactgg gccgctctgc
     2461 gtgaaaaagc gttgaatttt ggcgaggctg agcaggcgct gctgacagga cacgctttcc
     2521 accctgcgcc taagtctcat gaaccgttta accggcggga ggctgaacgc tacctgcctg
     2581 atatggcacc ccacttccca ctgcggtggt tttcggtgga taaaacgcaa atcgccggtg
     2641 aaagtttgca tctcaacctt caacagcggc tgacgcgatt tgccgcagag aatgcgcctc
     2701 agttactcaa cgaattaagt gacaaccaat ggctgttccc gttgcacccg tggcaggggg
     2761 aatatctttt gcagcagggg tggtgccagg cacttgttgc taaagggctg attaaagact
     2821 taggtgaggc cggcacgtcg tggctgccga ccacctcttc ccgttccctc tactgtgcca
     2881 ccagccgcga tatgatcaag ttctccctga gcgtacggct gaccaactcc atccgtactc
     2941 tgtccgtgaa agaagtgaag cgtgggatgc gcctggcacg cctggctcaa accgacggct
     3001 ggcagatgct acaggtccgc ttcccgactt tccgggtaat gcaggaggat ggctgggccg
     3061 ggctgctcga tcttaacggc aacatcatgc aggaaagtct gtttgccctg cgtgaaaatc
     3121 tgctggtgga tcagccgaaa agccagacta acgtactggt ctccctgact caggccgcac
     3181 ctgatggcgg tgattcgctg ctggtttcgg cggtaaaacg cctgagcgat cgcctcggta
     3241 tcactgtgca acaggccgcc catgcatggg ttgatgcata ctgtcagcaa gtgctaaagc
     3301 cgctgtttac ggctgaagcg gattacggcc tggtgctgct ggcgcatcag caaaatattc
     3361 ttgtccagat gcttggggat ctgccggtcg gatttattta ccgtgactgt cagggcagcg
     3421 cttttatgcc tcacgcgaca gactggctcg attctattgg cgaggcgcag gcggaaaata
     3481 ttttcaccca tgagcagttg ctgcgctatt tcccttatta cctgctggtt aactccactt
     3541 ttgctgtgac cgctgcgctg ggggctgccg ggctggacag cgaatcgaat ctgatggctc
     3601 gtgtacgagc atcgctggct gaagtgcgtg atcaggtgac tcataaaacc tgtctcaact
     3661 acgtgctgga aagtccgtac tggaacgtaa aaggtaactt tttctgttat ctgaacgatc
     3721 ataacgagaa caccattgtt gacccttcgg tgatctattt cgatttcgct aacccgctgc
     3781 aggctcagga ggtctgaatg tctgaggcaa acattattca cagcagatat ggactgcgct
     3841 gtgaaaaact cgacaagccc ctgaatcttg gctggggact ggacaatagc gcggtgttgc
     3901 actgtcccgg ggagctgccg acagggtggc tgtgcgatgc gctggatcag atatttatcg
     3961 ccgcaccaca actgtcagca gtggctctcc cctgggccga atggcgtgag gagccacagg
     4021 cgctgacgct ttttgggcag gtaaaaagcg acattatcca tcgtactgcc ttctggcagt
     4081 taccgttatg gttgagttca ccggcaaacc gggcctccgg tgaaatggtt tttgatgcag
     4141 agcgtgagat ttatttcccg cagcgcccac cccgtccaca gggtgaggtt taccgccgtt
     4201 acgatccacg tattcgcagg atgctgagtt tccgcattgc cgatcccgtt tctgatgcag
     4261 aacgtttcac ccgctggatg aatgatccgc gcgttgagta tttctgggag caaagtggct
     4321 cactggaggt acagaccgcc tatctggagc gccagctcac cggtaaacat gcattcccgc
     4381 tgattgggtg cttcgacgat cggccattca gctatttcga aatctactgg gcggcggaag
     4441 accgcattgg tcgtcactat tcgtggcagc cctttgaccg tggcctgcat ctgctggttg
     4501 gtgaacagca atggcgcggg gcccactatg tgcaaagctg gctgcgcggg ctgacacatt
     4561 acctgctgct ggatgagccc cgtacgcagc gcacagtact ggagccacgc accgataacc
     4621 agcgcctgtt ccgccatctt gagcctgcgg gataccggac aattaaagag ttcgacttcc
     4681 cacataagcg ctcgcgcatg gtgatggcgg atcgtcatca cttcttcacg gaggtcggtc
     4741 tgtaatgaat cacaaggact gggattttgt taaccgtcgg ctggtggcaa aaatgttgtc
     4801 tgagatggag tatgagcagg ttttccacgc ggaatctcag ggcgatgacc actactgcat
     4861 taacctgccg ggagcacaat ggcgcttcat cgctgaacgt ggtatctggg gctggctgtg
     4921 gattgatgcc caaacgttgc gctgcacgga cgaaccagta ctggcgcaga cgctgctgat
     4981 gcagctaaag ccggtgctgt caatgagtga tgccactgtt gcagagcata tgcaggattt
     5041 gtatgccacg ctactgggcg acctgcaact gctgaaggcc cgtcgcggac tgagtgccag
     5101 tgacctgatt aatcttgatg ccgatcgtct gcaatgcctg ctgagcggtc atcctaaatt
     5161 cgtttttaat aaaggtcgcc gtggctgggg caaagaggcg ctggaacgat atgcgccaga
     5221 gtataccaac accttcagac tgcactggct ggcggtaaaa cgtgaacata tgatctggcg
     5281 ctgcgataac gatctggata ttcagcagtt gttgactgcc gcaatggatc cgcaggagtt
     5341 tacacgcttc agtcaggtct ggcaggaaaa cgggctggat cacaactggc tgccgctgcc
     5401 ggtacatccg tggcagtggc agcaaaaaat cgccacagac ttcatcgctg attttgccga
     5461 aggcaggatg gtgtctctcg gcgagtttgg cgaccagtgg ctcgcccagc agtcgctgcg
     5521 taccctgacc aacgccagcc gccggggagg gctggatatc aagctgccgc tgaccatcta
     5581 caatacctca tgctaccggg ggatccctgg cagatatatc gctgccggac cactggcttc
     5641 acgctggctg caacaggttt ttgcgaccga cgccaccctg gtgcaaagcg gcgcagtgat
     5701 ccttggtgaa ccggctgcag gctatgtgtc ccatgaaggc tatgccgcgc ttgcccgggc
     5761 tccctatcgc taccaggaaa tgcttggtgt tatctggcgg gagaatccgt gccgctggct
     5821 gaaaccggat gaaagtcctg ttctgatggc aacactgatg gagtgcgacg aaaacaatca
     5881 gccgctggca ggcgcatata tcgaccgctc cggactggac gctgaaacct ggcttacgca
     5941 attgttccgg gtggttgtgg ttccgctgta tcacctgctt tgccgctacg gtgtcgcgct
     6001 tattgcacac ggacaaaata taacgctcgc catgaaagag ggggtaccac agcgtgttct
     6061 gctgaaagac ttccagggcg atatgcggct ggtgaaagaa gcgtttcccg aaatggactc
     6121 tttgcctcag gaggtgcgtg atgtcacatc ccgtctgagt gcggactact taatccatga
     6181 tttgcagacg ggtcacttcg tgacagtact gcgttttatt tcgccactga tggttcgtct
     6241 tggcgtacct gaaaggcgat tttatcaact gctggcagca gtgttgagtg attacatgaa
     6301 caaacatcca caaatggcag agcgttttgc gcttttctca ctcttcaggc cacaaatcat
     6361 tcgcgtggtg ctgaacccgg taaaactgac ctggccggac ctggatggcg gcagtcgcat
     6421 gctgccgaat taccttgaga atctgcaaaa tccgctgtgg ctggtaactc aggaatatga
     6481 atcatgaaaa aaagtgtcga ttttattggt gtcgggacag ggccatttaa tctcagcatt
     6541 gctgcattgt cacatcagat cgaagaactg aactgtctct tctttgacga acatcctcat
     6601 ttttcctggc atccgggtat gctggtaccg gattgtcata tgcagaccgt ctttctgaaa
     6661 gatctggtca gtgcagttgc acctacaaat ccctacagct ttgttaacta tctggtgaag
     6721 cacaaaaagt tctatcgctt ccttacaagc aggctacgta cagtatcccg tgaagagttt
     6781 tctgactatc tccgctgggc tgctgaagat atgaataacc tgtatttcag tcataccgtt
     6841 gaaaatattg atttcgacaa aaaaagtcga ttgtttctgg ttcagaccag tcggggagaa
     6901 tattttgccc acaatatctg ccttggtaca ggaaaacaac cttatttacc accctgtgtg
     6961 aagcatgtga cacaatcctg tttccatgcc agtgaaatga atcttcgtcg gccggacctg
     7021 agtggaaaac ggataaccgt ggttggtgga ggacagagtg gtgcagacct gttccttaat
     7081 gcattacgcg gggaatgggg agaagcggcg gaaataaact gggtctcacg gcgtaataat
     7141 tttaacgcac tggatgaggc tgcttttgct gatgagtatt ttacacctga atatatttca
     7201 ggcttctccg gactgaagga agatattcgc catcagttac tggatgagca gaaaatgaca
     7261 tcggatggca tcactgccga ttctttactg accatttatc gtgagttgta ccaccgtttt
     7321 gaagttctga gaaaaccaag aaatatccgt ctgctaccca gtcgctcggt aacaactctg
     7381 gaaagtagtg gtccgggctg gaggttattg atggaacatc atctggatca gggcagagag
     7441 agcctggaaa gtgatgtggt gattttcgcc acaggttacc gttctgcatt gccacaaata
     7501 cttccctcac tgatgccctt gatcaccatg cacgataaga acacctttaa agtgcgtgat
     7561 gacttcactc tggaatggag tggcccgaaa gaaaataaca tctttgcggt caacgccagc
     7621 atgcaaactc atggcatcgc cgaaccccag ctcagcctga tggcctggcg atctgcacgt
     7681 attcttaatc gcgtactggg gcgtgattta ttcgatctca gtatgccgcc cgcactgatt
     7741 cagtggcgca gcggcagccg gaaaaaacca cagccggagg ctgcttcttt aactcactat
     7801 acagcaaata ttcaggaata acgatgatgc gcaaaaagta tatgccccgg gctcttggtc
     7861 cgctgcttct tgtcgtgctg tcaccagctg tcgctcagca aaacgatgat aatgagatca
     7921 tagtgtctgc cagccgcagc aatcgaactg tagcggagat ggcgcaaacc acctgggtta
     7981 tcgaaaatgc cgaactggag cagcagattc agggcggtaa agagctgaaa gacgcactgg
     8041 ctcagttaat ccccggcctt gatgtcagca gccagagtcg aaccaactac ggtatgaaca
     8101 tgcgtggccg cccgctggtt gtcctgattg acggtgtgcg cctcaactct tcacgttccg
     8161 acagccgaca actggactct gtcgatcctt ttaatatcga ccatattgaa gtgatctccg
     8221 gcgcgacggc cctgtacggt ggcgggagta ccggagggtt gatcaacatc gtgaccaaaa
     8281 aaggtcagcc ggaaaccatg atggagtttg aggctggcac aaaaagtggc tttaacagca
     8341 gtaaagatca cgatgagcgc attgccggtg ctgtctccgg cggaaatgac catatctccg
     8401 gacgtctttc cgtggcatat cagaaatttg gcggctggtt tgacggtaac ggcgatgcca
     8461 ccctgcttga taacacccag accggcctgc agcactccaa tcggctggac atcatgggaa
     8521 ccggtacgct gaacatcgat gaatcccggc agcttcaact gataacgcag tactataaaa
     8581 gtcagggaga cgacaattac gggcttaatc tcgggaaagg cttttccgcc atcagcggga
     8641 gcagcacacc atacgtcagt aaggggctga attctgaccg cattcccggc actgagcggc
     8701 atttgatcag cctgcagtac tctgacagtg atttcctggg acaggaactg gtcggtcagg
     8761 tttactaccg cgatgagtcg ttgcggtact acccgttccc gacggtaaat gcgaataaac
     8821 aggcgacggc tttctcctcg tcacagcagg ataccgacca gtacggcatg aaactgactc
     8881 tgaacagcca actgatggac ggctggcaaa tcacctgggg gctggatgct gagcatgagc
     8941 gctttacctc caaccagatg ttcttcgatc tggctcaggc aagtgcttcc ggagggctga
     9001 acaaccataa gatttacacc accgggcgct atccgtcata tgacatcacc aatctggcgg
     9061 ccttcctgca atccagctat gacattaatg atatttttac cgttagcggt ggcgtacgct
     9121 atcagtatac tgagaacagg gtagatgatt tcatcgacta cacgcagcaa cagaagattg
     9181 ctgccgggaa ggcgatatct gccgacgcca ttcctggtgg ttcggtagat tacgataact
     9241 ttctgttcaa tgctggtctg ctgatgcaca tcaccgaacg tcagcaggca tggttcaatt
     9301 tttcccaggg ggtggcattg ccggatccgg ggaaatatta tggtcgcggc atctatggtg
     9361 cagcagtgaa cggccatctt cccctgacaa agagcgtgaa cgtcagcgac agtaagctgg
     9421 aaggcgtgaa agtcgattct tatgaactgg gctggcgctt tatcggtgac aacctgcgga
     9481 ctcaaatcgc ggcatattac tcgctttcca ataagagcgt ggaaaggaat aaagatctga
     9541 ccatcagtgt gaaggacgac aggcgccgta tttacggcgt ggaaggtgcg gtggactacc
     9601 tgatcccgga tactgactgg agtaccggtg tgaacttcaa tgtgctgaaa accgagtcga
     9661 aagtgaacgg tcaatggcaa aaatatgacg tgaaggaatc aagtccatcg aaagcgacag
     9721 cttacattaa ctgggcgccg gaaccgtgga gtctgcgtgt acagagcacc acttctttcg
     9781 acgtaagcga tgcagagggt aacgatatta atggttacac taccgtcgat tttatcagta
     9841 gttggcagct tccggtggga acactcagct tcagcgttga gaacctcttc gaccgtgact
     9901 ataccactgt ctggggacag cgtgcacctc tgtactacag cccgggttac ggccctgctt
     9961 cactgtacga ctacaaaggc cggggccgaa cctttggtct gaactactca gtgctgttct
    10021 gacggggatg cttttgcaac aagggtatgc tgtaaacaac atggtccttg gtggccatgt
    10081 tgtttacagc cagtataata tacagcctta acaagatatt ccgtctccgg aatgggctgt
    10141 tcaccactga ataacattat gtctgtttta tggatctttg actgaaattt atcgcatatc
    10201 ctcatttcat tgtccgtctg aaaactaacc ttacaagtaa gcgtcgtctc agcaccgtct
    10261 ggcagatcct gaaattcctg agaggatgat ctgcgcccaa tatcctgagc cggaactttg
    10321 ctgcaatgaa ggccaacgaa aaatggggga gtatgttata gagtacttgc tacagggtaa
    10381 aaagaggtac ctgtcgctgg ttctccgttc agtttaaccg ggagattttc tcctgcgcct
    10441 gtcataaagt ccggtaatgg agattgttaa tacgatggtg cacgatgcat cgtcagtgcc
    10501 cggcccggag gatgccccgt agctacatac ggatcagggt tggcaatatc gaatggcagg
    10561 ctataggcaa agttaaagcc ccatggagta gcacaaaata tgccgcacaa aggaaacggt
    10621 ctgaataacg cagtgatgga gaacttcttc ggcacactgc aaaatcgcat cgtggtcgat
    10681 cgctaattcg tcgtgaatca ctgaacgatg ttccatgcga acagatattc gatcatatcg
    10741 aaatattaaa cattacccct ccccacattg ggcaaaaaaa aacaaaaaac gatcaattaa
    10801 cgatcaatta gtattgattc acataatctc tcatcattaa tgagaacatt gtcacatatt
    10861 tacaatatag aactgttact tttagcaagc tgttagtaac caaccgttac taataatttt
    10921 aattcttaat actttgttca ttcaataaat gagttgagag aatatatgaa taaaatatac
    10981 tcccttaaat atagtgctgc cactggcgga ctcattgctg tttctgaatt agcgaaaaga
    11041 gtttctggta aaacaaaccg aaaacttgta gcaacaatgt tgtctctggc tgttgcaggt
    11101 acagtaaatg cagcaaatat tgatatatca aatgtatggg cgagagacta tcttgatctt
    11161 gcacaaaata aaggtatttt ccagcccgga gcaacagacg taacaatcac tttaaaaaac
    11221 ggagataaat tctctttcca taatctctca attccggatt tttctggtgc agcagcgagt
    11281 ggcgcagcta ccgcaataag aggttcttat agtgttactg ttgcacataa caaaaagaac
    11341 cctcaggccg cagaaactca ggtttacgct cagtcttctt acaaggttgt tgacagaaga
    11401 aattccaatg attttgagat tcagaggtta aataaatttg ttgtggaaac agtaggtgcc
    11461 accccggcag agaccaaccc tacaacatat tctgatgcat tagaacgcta cggtatagtc
    11521 acttctgacg gttcaaaaaa aatcataggt tttcgtgctg gctctggagg aacatcattt
    11581 attaatggtg aatccaaaat ctcaacaaat tcagcatata gccatgacct gttaagtgct
    11641 agtctatttg aggtcaccca atgggactc
//
LOCUS       DAJTQY010000111        11631 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.111, whole genome shotgun sequence.
ACCESSION   DAJTQY010000111 DAJTQY010000000
VERSION     DAJTQY010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11631
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.111"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(282..357)
                     /locus_tag="OO349_003327"
     tRNA            complement(282..357)
                     /locus_tag="OO349_003327"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(322..324),aa:Ile,seq:cat)
     gene            916..3168
                     /locus_tag="OO349_003328"
     CDS             916..3168
                     /locus_tag="OO349_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5868288.1"
                     /translation="MFSIKPGPGNLPIDNPTLLSWNITDGDLNSKFNTLEYLNCITNI
                     INACGVYPQDLKDREIISTFHAEKVINDLLKNDYKISLSPDTTYRELNKAAQSSITAP
                     DRIGEGKTWVYQRDTMVERGDNSGVHQYGPAEHFTHIISDKPSPKDKYVAYAINIPDY
                     ELAADVYNINVTSPSGQQETFKILINPEHLRQTLERKSLTAVQKSQCEIITPKKPGEA
                     ILHAFNATYQQIRENMSEFARSHYGYIQIPPVTTFRADGPETPEEEKGYWFHAYQPED
                     LCTIHNPMGDLQDFIALVKDAKKFGIDIIPDYTFNFMGIGGSGKNDLDYPSADIRAKI
                     SKDIESGIPGYWQGQVLIPFIKDPVTKERKQIHPEDIHLTAKDFEASKDNISKDEWKN
                     LHALKEKSLNGMPKTTPKSDQVIMLQNQYVREMRKYGVRGLRYDAAKHSKHEQIERSI
                     TPPLKNYNERLHNTNLFNPKYHEKTVMNYMEYLVTCQLNEEQMSSLLYERDDLSAIDF
                     SLLMKTIKAFSFGGDLLTLASKPGSTISSIPSERRILININHDFPNNGNLFNDFLFNH
                     QQDEQLAMAYMAALPFSRPLVYWDGQVLKSTTEIKNYDGSTRVGGEAWLNKGCSTYQQ
                     LYNEFHALYIDKAGIWSAFEGVFATKNVLAFSRGDSVNINHSPHDGLVIINKGNEEVE
                     GTWPNKLQPGIYKNMGSNSVNIIINNTRKIIPPCKVFTLRGGSLNINIPRRSALLLGK
                     TGEPPNYLYL"
     gene            3504..4481
                     /gene="csiD"
                     /locus_tag="OO349_003329"
     CDS             3504..4481
                     /gene="csiD"
                     /locus_tag="OO349_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation induced protein CsiD"
                     /protein_id="HCR5868289.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            4501..5769
                     /gene="lhgO"
                     /locus_tag="OO349_003330"
     CDS             4501..5769
                     /gene="lhgO"
                     /locus_tag="OO349_003330"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HCR5868290.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSQLEMERM
                     RALWERTAANGIEREWINAVELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILSSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            5792..7240
                     /gene="gabD"
                     /locus_tag="OO349_003331"
                     /pseudo
     CDS             5792..7240
                     /gene="gabD"
                     /locus_tag="OO349_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
     gene            7248..8534
                     /gene="gabT"
                     /locus_tag="OO349_003332"
     CDS             7248..8534
                     /gene="gabT"
                     /locus_tag="OO349_003332"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HCR5868291.1"
                     /translation="MRRNSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGRE
                     YLDFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGD
                     FAKKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGEYHGRTHYTLALTGKVNPYSAG
                     MGLMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIMIEPVQGEGGFYA
                     ASPAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGF
                     PLAGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANDLGQKLK
                     DGLLAIAEKHPEIGDVRGLGSMIAIELFEDGDHSKPDAKLTAEIVARARDKGLILLSC
                     GPYYNVLRILVPLTIEDAQIRQGLEIIGQCFDEAKQ"
     gene            8772..10172
                     /gene="gabP"
                     /locus_tag="OO349_003333"
     CDS             8772..10172
                     /gene="gabP"
                     /locus_tag="OO349_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HCR5868292.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAD
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDMPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLDPWLTWLVISFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            10193..10855
                     /gene="csiR"
                     /locus_tag="OO349_003334"
     CDS             10193..10855
                     /gene="csiR"
                     /locus_tag="OO349_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HCR5868293.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLQ
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLPRDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(10856..11305)
                     /gene="kbp"
                     /locus_tag="OO349_003335"
     CDS             complement(10856..11305)
                     /gene="kbp"
                     /locus_tag="OO349_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HCR5868294.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(11389..11547)
                     /locus_tag="OO349_003336"
     CDS             complement(11389..11547)
                     /locus_tag="OO349_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HCR5868295.1"
                     /translation="MGFWRIVITIILPPIGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
BASE COUNT         2992 a         2855 c         2900 g         2884 t
ORIGIN      
        1 tccggaacaa tttgaaaacc agaacctcgc ttaggcctgt gtccatatta cgtgggtagg
       61 atcaatttgc ccgcttggca tagcgggcat tttttccagg tacttttgaa tgagtactga
      121 tgaataaata cattgcagtg gcgtgccacg taccaaaaca ccagccctca ttcgaaacca
      181 cccaccgcac ttcttccttg aaatggcgtt agtcatgaaa tatagaccgc catcgagtac
      241 cccttgtacc cttaactctt cctgatacat gaatgatgat ttggtggccc ttgctggact
      301 tgaaccagcg accaagcgat tatgagtcgc ctgctctaac cactgagcta aagggcctta
      361 agtatgcaat aacaatactt ataaaccacg caataaacat gatgatctta tgatgtaaat
      421 aacagatttt tatgcgttcc cattcacctt gggtcgtgat tacacgcgac ataaaacccg
      481 acaccgcctc cattcgcaaa gtcgatactc gcagtcaaca agcaaatgtt aataattagc
      541 actatctata gttatcatcg attcaatgat actttgtaat gattttgtat ctaataatat
      601 aactttatta cattagctga agagttttcg catcattatg atatctgtta cttttcactc
      661 cataaaaata aacttcatat agcaatatat tctttcatag atcttattct gctaatcatt
      721 agtttcgtat gcgcgatttt tgacagttgc ctctccagac cacatcgata attaaaaaac
      781 agatttaaac attatccttt tccatataga tattggataa aaataggaca tctgtttgca
      841 attactttca caacaattaa acatttttat gttttcgtat acatcatatt actctaccat
      901 tagaggaacg ttattatgtt ttctataaaa ccaggacctg gaaatttacc tatcgacaac
      961 cccacattgt tatcatggaa cattactgat ggggatctaa attccaaatt taatacatta
     1021 gaatatctaa actgtataac aaatattatt aatgcttgtg gagtttatcc tcaggattta
     1081 aaagacagag aaattatatc aacttttcac gcagaaaaag tcattaatga tctgttaaaa
     1141 aacgattata aaatttccct ttctccagat acaacttacc gagagctgaa taaagctgca
     1201 cagagtagca ttacagcgcc agacaggata ggagaaggga aaacatgggt ttatcaacga
     1261 gatacaatgg ttgaaagagg tgataacagt ggtgttcatc agtatggtcc agctgaacat
     1321 ttcactcaca ttatatctga caaaccgtcc ccaaaagata agtatgttgc atatgctatt
     1381 aacattcctg actatgagct agcagccgat gtatataata ttaacgtgac gtcaccttcc
     1441 ggacagcaag aaacatttaa aatactaatc aatccagaac atctacggca aacacttgag
     1501 cgtaaatctc ttactgctgt tcagaaatca caatgtgaaa tcatcacccc caaaaaacct
     1561 ggcgaagcga ttcttcatgc ttttaatgcc acctaccagc aaatcaggga aaatatgtct
     1621 gagtttgcac gttcccatta tgggtatata caaatccctc cagtgacaac tttccgcgcc
     1681 gacggaccag aaactcccga agaagaaaaa ggttactggt ttcacgctta tcaacccgaa
     1741 gatctttgta ccatccataa tccaatgggg gatttgcagg attttatcgc attggttaaa
     1801 gatgctaaaa aatttggtat cgatatcatt cctgattata cctttaactt tatgggaatc
     1861 ggggggagtg gtaaaaatga cctggattat ccctctgctg atatacgagc gaagatcagt
     1921 aaagatatag aaagtggtat ccctggctat tggcaaggtc aggttttgat tccattcatt
     1981 aaagatccag taacaaaaga acgtaaacaa atccatccag aagatataca tctcactgca
     2041 aaagacttcg aagcaagtaa agataacatc tctaaggatg aatggaaaaa cctccatgca
     2101 ttaaaagaaa agagtttaaa tggaatgcct aaaacaaccc ccaaaagtga ccaggttatt
     2161 atgttgcaaa atcaatacgt tcgtgaaatg cgaaaatatg gcgtacgagg tttacgttat
     2221 gatgcagcaa aacactcaaa acatgaacaa atagaaagat caataacccc accgcttaaa
     2281 aattataatg agcgattaca caatactaac ttatttaacc caaaatatca tgaaaaaacc
     2341 gttatgaatt acatggaata tctggtaact tgtcagttga atgaagaaca aatgtcatcg
     2401 ctgctttatg aaagagatga tttaagcgcc attgattttt cattgctcat gaagacgata
     2461 aaagcctttt cattcggggg agatctccta acccttgcat caaaaccggg ttccacaatc
     2521 tcaagcatcc catcagaaag acggatattg attaacatta accacgattt tcctaacaat
     2581 ggcaatcttt tcaatgactt tctatttaac catcaacaag atgaacaatt agcaatggca
     2641 tatatggccg ctctcccgtt cagcaggcct ttagtttact gggatggcca ggtattaaaa
     2701 tcaacgactg aaattaaaaa ttatgatggg tccacgcgtg tcggtggtga ggcgtggctt
     2761 aataaaggtt gctctaccta tcagcagctc tacaatgaat tccacgcatt atatatagat
     2821 aaagcaggaa tatggagcgc atttgagggt gtatttgcaa ctaaaaacgt tctggccttt
     2881 agtcgtgggg attctgtgaa cattaatcac tctcctcatg atggactagt tataataaat
     2941 aaaggaaacg aagaagttga aggtacctgg cctaacaaat tgcaacctgg aatatataaa
     3001 aacatgggga gtaatagcgt taacattatt attaataata cccgaaaaat tatcccccct
     3061 tgtaaagtat ttacgcttag aggcggatct ctaaatatca atattcctag gcgtagcgct
     3121 cttcttttag ggaaaacagg agaaccgccg aactatctct atttataatt taatgttata
     3181 tctgccccga taaaacgggg cagataatat gtttagttta ctaacggtca ttttgtagtg
     3241 aagccattta ctgtttttta tcgaccagat aatctgttct ctaatgttaa ctccccctaa
     3301 cctgttgctt tagttattca tttcctgtct cactttgcct taatacccta cgttaaatgt
     3361 tactaatttg ttgcttttga tcacaataag aaagcaatat gtcgcttttg tgcgcatttt
     3421 tcagaaatgt agatattttt agattatggc tacgaaatga gcatcgccat gtcaccctac
     3481 atctcataag aggatcgctt ctgatgaatg cactgaccgc cgtacaaaat aacgctgtcg
     3541 attcaggcca ggactatagc ggattcactc tcatcccgtc ggcgcaatcc ccgcgtctgc
     3601 tggaactcac cttcaccgaa cagacgacca aacagtttct cgaacaggtt gccgaatggc
     3661 ccgtgcaggc gctggagtac aaatcgtttc tgcgttttcg ggtaggcaaa attcttgacg
     3721 atctgtgtgc gaatcagctg caaccattgc tgttgaagac cctgctaaac cgtgctgaag
     3781 gtgcgctgtt gatcaatgcg gtaggtatcg atgatgtggc gcaggcagat gagatggtga
     3841 agctggcaac ggcggtagcg catctgattg gtcgctcaaa tttcgatgcg atgagcggtc
     3901 agtattacgc gcgtttcgtg gtgaaaaatg tcgataactc agacagctat ctgcgtcagc
     3961 cgcaccgcgt gatggaactg cacaacgacg gcacgtatgt cgaagagatc accgattacg
     4021 tgctgatgat gaaaatcgat gagcaaaaca tgcagggtgg aaattcgttg ctgctgcatc
     4081 tcgatgactg ggaacatctg gaccactatt tccgccatcc actggcgcgt cgcccgatgc
     4141 gctttgccgc gccgccgagc aaaaacgtca gcaaagatgt tttccatcca gtgttcgacg
     4201 ttgatcaaca gggtcgcccg gtgatgcgct atatcgacca gttcgtccag ccaaaagatt
     4261 ttgaagaagg cgtgtggttg agcgagcttt ccgatgccat tgaaaccagc aaaggcattc
     4321 tttctgtacc cgttcccgtt ggcaaattcc tgttgataaa caacctgttc tggctgcacg
     4381 gtcgcgaccg ctttactccg cacccggatc tgcgccgtga actgatgcgt cagcgtggct
     4441 atttcgctta cgccactcac cactaccaga cgcatcagta agcgcaaagg aattgagcgg
     4501 atgtatgatt ttgtgattat tggcggcggc atcatcggca tgtcgaccgc catgcaactg
     4561 attgatgttt atccggatgc ccgcattgcg ttgctggaaa aagagtccgg cccggcctgt
     4621 caccagacgg gccacaacag cggcgtgatc catgccgggg tctattacac gcccggcagc
     4681 ctgaaggcgc agttttgcct ggcggggaac cgtgccacca aagccttttg cgatcaaaac
     4741 ggcattcgct atgacaactg cggcaagatg ctggtcgcca cgtctcagct cgaaatggaa
     4801 cggatgcgcg ccttatggga acgcacagcg gcgaacggta tcgagcgcga gtggataaac
     4861 gccgtggaac tgcgcgagcg cgaaccgaat atcaccgggc ttggtggcat ttttgtgccg
     4921 tccagcggca ttgtcagcta ccgcgaagtg acggcggcaa tggcaaaaat cttccaggcc
     4981 agaggcggcg agattattta taacgccgaa gtcagcgccc tcagtgagca taaaaacggc
     5041 gtggtgatac gcacccgtca gggcggtgaa tatgaagcat cgacgctgat tagctgttcc
     5101 gggctgatgg ctgaccggct ggtgaaaatg ctcggcctcg aaccgggctt tattatctgc
     5161 ccgttccgtg gcgagtattt ccgccttgcg ccggagcata accagattgt taaccacctg
     5221 atttacccca ttcccgaccc cgcgatgcca tttttgggcg tgcatctcac ccgcatgatc
     5281 gacggcagcg tgaccgtcgg gccaaacgcg gtgctggctt tcaaacgcga aggctatcgc
     5341 aagcgcgact tctcatttag tgacacgctg gagattttga gctcgtcggg gattcgccgg
     5401 gtgctgcaaa accatctacg ctcaggactg ggcgagatga aaaactcgct gtgcaaaagc
     5461 ggctatctgc ggctggtgca aaagtattgt ccccggcttt cgttaagcga tctccagccc
     5521 tggcccgccg gtgtgcgggc gcaggcagta tcgccggacg gcaagctgat tgacgatttt
     5581 ctgtttgtca ccaccccacg cacgatccac acctgcaatg cgccctcccc ggcagcgaca
     5641 tcagcaattc ctattggtgc gcatattgtc agcaaggtac aaacgctgtt ggcaagccag
     5701 agtaaccccg gacgcacgct gcgagcggca cgtagtgtgg atgccttaca cgccgcattt
     5761 aatcaataac ctttgaaaac aggacgtaac aatgaaactt aacgacagta acttattccg
     5821 ccagcaggcg ttgattaacg gggaatggct ggacgccaac aatggtgaag tcatcgacgt
     5881 caccaatccg gcgaacggcg acaagctggg tagcgtgccg aaaatgggcg cagatgaaac
     5941 ccgcgccgct atcgacgccg ccaaccgcgc cctgcccgcc tggcgtgcgc tcaccgccaa
     6001 agaacgcgcc accattctgc gcaactggtt caatttgatg atgcagcatc aggacgattt
     6061 agcgcgcctg atgaccctcg aacagggtaa accgctggct gaagcgaaag gcgaaatcag
     6121 ctacgccgcc tccttcattg agtggtttgc tgaagaaggc aaacgtattt atggctacac
     6181 cattcctggt catcaggccg ataagcgcct gattgttatc aagcagccga ttggcgtcac
     6241 cgccgccatc acgccgtgga actttccggc ggcgatgatt acccgtaaag ccggtccggc
     6301 gctggcggca ggctgcacca tggtgctgaa gcccgccagt cagacgccgt tctctgcgct
     6361 ggcgctggcg gaactggcga ttcgcgcggg cattccggct ggcgtgttta acgtggtcac
     6421 cggttcggca ggcgcggtcg gtaatgaact gaccagcaac ccgctggtac gcaaactgtc
     6481 gtttaccggt tagaccgaga ttggccgcca gttaatggaa cagtgcgcga aagacatcaa
     6541 aaaagtgtcg ctggagctgg gcggcaacgc gccgtttatc gtctttgacg atgccgatct
     6601 cgataaagcc gtggaaggcg cgctggcctc gaaattccgc aacgccgggc aaacctgcgt
     6661 ctgcgccaac cgtttatacg tgcaggacgg cgtgtatgac cgctttgccg aaaaattaca
     6721 gcaggcggtg agcaaactgc acatcggcga cgggctggag aaaggcgtca ccatcgggcc
     6781 gctgatcgat gaaaaagcgg tagcaaaagt ggaagagcat attgccgatg cgctggagaa
     6841 aggtgcgcgt gtggtttgcg gcggtaaagc gcacgaacgc ggcggcaact tcttccagcc
     6901 aaccattctg gtggacgttc cggccaacgc caaagtgtcg aaagaagaga cgttcggccc
     6961 cctcgcccca ctgttccgtt ttaaagatga agccgatgtg attgcacaag ccaatgacac
     7021 cgagtttggt cttgccgcct atttctacgc ccgtgattta agccgcgtct tccgcgtcgg
     7081 cgaggcgctg gagtacggca tcgtcggcat caataccggc attatttcca atgaagtggc
     7141 cccattcggc ggcatcaaag cctcgggtct gggtcgtgaa ggttcgaagt atggcatcga
     7201 agattactta gaaatcaaat atatgtgcat cggtctttaa ctggagaatg cgaaggaaca
     7261 gcaataaaga gttaatgcag cgccgcagtc aggcgattcc ccgtggcgtt gggcaaattc
     7321 acccgatttt cgctgaccgc gcggaaaact gccgggtgtg ggacgttgaa ggccgtgagt
     7381 atcttgattt cgcgggcggg attgcggtgc tcaataccgg gcacctgcat ccgaaggtgg
     7441 tggccgcggt ggaagcgcag ttgaaaaaac tgtcgcacac ctgcttccag gtgctggctt
     7501 acgagccgta tctggagctg tgcgagatta tgaatcagaa ggtgccgggc gatttcgcca
     7561 agaaaacgct gctggttacg accggttccg aagcggtgga aaacgcggta aaaatcgccc
     7621 gcgccgccac caaacgtagc ggcaccatcg cttttagcgg cgagtatcac gggcgcacgc
     7681 attacaccct ggcgctgacc ggcaaggtga atccgtactc tgcgggcatg gggctgatgc
     7741 cgggtcatgt ttatcgcgcg ctttatcctt gcccgctgca cggcataagc gaggatgacg
     7801 ctatcgccag catccaccgg atcttcaaaa atgatgccgc gccggaagat atcgccgcca
     7861 tcatgattga gccggttcag ggcgaaggcg gtttctacgc cgcgtcgcca gcctttatgc
     7921 agcgtttacg cgctctgtgt gacgagcacg ggatcatgct gattgccgat gaagtgcaga
     7981 gcggcgcggg gcgtaccggc acgctgtttg cgatggagca gatgggcgtg gcgccggatc
     8041 ttaccacctt tgcgaaatcg atcgcaggcg gtttcccgct ggcgggcgtc accgggcgcg
     8101 cggaagtgat ggacgctgtt gcaccgggcg gtctgggcgg cacctatgcc ggtaacccga
     8161 ttgcctgcgt ggctgcgctg gaagtgttga aggtgttcga gcaggaaaat ctgctgcaaa
     8221 aagccaacga tctggggcag aagctaaaag acggattgtt ggcgatagcc gaaaaacacc
     8281 cggagatcgg cgacgtacgc gggctgggat cgatgattgc catcgagctg tttgaagatg
     8341 gcgatcacag caaaccggac gcaaaactca ccgccgagat cgtggcacgc gcccgcgata
     8401 agggcctgat tcttctctcc tgcggcccgt attacaacgt gctgcgcatc cttgtaccac
     8461 tcaccattga agacgctcag atccgtcagg gtctggagat tatcggtcag tgttttgatg
     8521 aggcgaagca gtagcaccgc ccccatgcag gaggcgacgc tgcgcgtctt gtccggccta
     8581 cgagaatcca agtcggataa ggcgtttacg ccgcatccga caatctgtac gtgaacagga
     8641 ataaatctat gtcggccgga taaggcggag ccgctctccg gcaaaaagaa tcaataacaa
     8701 ttaaacgcgt gacccgacgc gggaaatgtc ggggcgctct cccaagtgac acactttcga
     8761 gaggattcag gatggggcaa tcatcgcaac cacacgagtt aggcggcggg ctgaaatcac
     8821 gccacgtcac catgttgtct attgccggtg ttatcggcgc aagtctgttt gtcggttcca
     8881 gcgtcgccat cgccgaagcg gacccggcgg tattactggc ctatctgttc gccggattac
     8941 tggtggttat gattatgcgg atgctggcgg aaatggcagt tgctacgccc gataccggtt
     9001 cgttttccac ctatgccgat aaagccattg gccgctgggc gggctatacc atcggctggt
     9061 tgtactggtg gttttgggta ctggttatcc cactggaagc caacatcgcc gccatgatcc
     9121 tgcactcgtg ggttccaggc attcccatct ggttattttc cctcgtcatt accctcgcct
     9181 taactggcag taatttatta agcgttaaaa actacggcga atttgagttc tggctggcgc
     9241 tgtgcaaagt catcgctatc ctggccttta ttttccttgg tgcagtcgca attagcggtt
     9301 tttaccctta tgccgaagtg agcgggatct caagattgtg ggatagcggc ggctttatgc
     9361 ccaacggttt cggtgcggta ttaagcgcga tgttgatcac catgttctcg tttatgggcg
     9421 cagaaattgt caccattgcc gccgcggaat ccgacatgcc ggaaaaacat attgtccgcg
     9481 ccaccaactc ggttatctgg cgtatttcta tcttctattt gtgctctatt tttgtcgtcg
     9541 tggcattaat accgtggaat atgccggggc tgaaagccgt tggatcttat cgctcggttc
     9601 tggaattgct caatattccc catgcgaaat taatcatgga ctgtgtgata ttactttccg
     9661 taaccagttg cctgaactcg gccctgtata ccgcctcaag gatgctctac tccttaagcc
     9721 gtcgcggtga tgctcccgcg gtaatgggta aaatcaaccg cagtaaaacc ccgtacgtgg
     9781 cggtgttact ctccaccggc gcagcattct taacggtggt ggtgaactat tacgcgcctg
     9841 cgaaggtatt taaatttctg atcgacagct ccggcgctat cgccctgctg gtttatttag
     9901 tcatcgccgt ttcacagttg cggatgcgca aaattctgcg agcagaagga agcgaaattc
     9961 gcttgcgaat gtggcttgat ccctggctca cctggctggt catcagcttt attacctttg
    10021 tgttggtagt gatgctattc cgtccggcgc aacagttaga agtgatctcc accggcttat
    10081 tagcgatagg gattatctgt accgtgccaa ttatggctcg ctggaaaaag ctggtattgt
    10141 ggcaaaaaac acccgttcat aatacgcgct gataatgatc aggagtcaca ccatgaccat
    10201 tacgtctctg gatggctatc gctggctgaa gaacgatatt attcgcggta attttcaacc
    10261 ggatgaaaaa ttacggatga gtttactgac atcgcgttat gcacttggcg ttgggccgtt
    10321 gcaggaagca ctttctcaac tggtggcgga acggctggtc acggtggtga atcaaaaagg
    10381 gtatcgggtg gcgtctatgt cagagcagga gctgctcgat attttcgacg cccgcgccaa
    10441 tatggaagcg atgctggtca gtctggcgat tgcccgcggt ggcgatgaat gggaggcaga
    10501 cgttctcgca aaagcgcatc tgctcagtaa gctggaagcc tgtgacgcca gcgagaaaat
    10561 gcttgatgag tgggatctgc gtcatcaggc gtttcataca gcaattgtgg cgggctgtgg
    10621 ttctcactat ttgctgcaaa tgcgtgaacg gttgtttgat ctggcggcgc gttatcgatt
    10681 tatctggctg cggcgaacgg tgctttcggt ggaaatgctg gaggataaac acgatcagca
    10741 ccagaccctg actgcggcgg tactgccgcg agatacagcg cgcgccagtg agttaatgcg
    10801 ccagcattta ctgacgccaa ttcccattat ccagcaggcg atggctggca attaactact
    10861 cttccggaat acgcaacact tgccccggat aaattttatc cgggcttttt agcatcggtt
    10921 tattcgcttc gaagatttta ttgtacagat tagcgttacc gtagacctgt ttggaaatgg
    10981 cactcagagt gtcgccagac ttaacggtat aaaactggct ggcagtggct ggtgtcgccg
    11041 ttttcacctg atcatcaaca ctggcaatac cggaaatatt ccccaccgca acaaggattt
    11101 tctccttcgc ctcctgactc aggccgtcac cagtgaccgt cgctttgccg tcggcaattt
    11161 gaatgttcac tttatcggca tcaggtatac cggttttgct cagatgctcc tgcaccttct
    11221 tcgcctgatc gtctttatcg tgctgacctg taaccgcatc ccagagtttt tctccggcat
    11281 ctttaacaaa attgaacaga cccatagcta cctcattcgt taacggaaac actcagaaag
    11341 tgtagcagag gaacgcggcg cgttaacgcc gcgggggtga gcacaggttt aatcgcgggt
    11401 ttgcacccag aacgcgtgaa tcaaaccggg gatataccca agcagtgtca gcagaatatt
    11461 aataatgaac gcccaaccga accctttacc gagcagcacg ccgatcggcg gcagaatgat
    11521 ggtgatgacg attctccaga aacccatatg tactccctat aagaaaatta ctcattgttt
    11581 aaaaagagat tttatctctt aagcgtagta attttagcgg agcctgccag t
//
LOCUS       DAJTQY010000112        11419 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.112, whole genome shotgun sequence.
ACCESSION   DAJTQY010000112 DAJTQY010000000
VERSION     DAJTQY010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11419
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.112"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            56..796
                     /locus_tag="OO349_003337"
     CDS             56..796
                     /locus_tag="OO349_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HCR5868296.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGNGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTIASKDVMTRTVKISGVGRPYLAEEGTETVG
                     VTGLTVAPASVSVKAGATTTLTFTVKPDGASDKAISVHSSDPQTASVTLSGLVATVKG
                     VKQGSVSIVGMTSDGEFVAVAAVTVSAP"
     gene            855..1241
                     /locus_tag="OO349_003338"
     CDS             855..1241
                     /locus_tag="OO349_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HCR5868297.1"
                     /translation="MFLKQDTFNYEKQSVVLSELSGLQRIEYLTFVQQRTAKFDAQEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNAEQSQDVETLCASIMTTWSYDALGAGAER
                     VLSLSGMGTIENAGDDDHEALTPEKS"
     gene            1250..1579
                     /locus_tag="OO349_003339"
     CDS             1250..1579
                     /locus_tag="OO349_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HCR5868298.1"
                     /translation="MQFVMRLAREFRRADWRRMLSDMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            1551..4289
                     /locus_tag="OO349_003340"
     CDS             1551..4289
                     /locus_tag="OO349_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR5868299.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAASDAERSSARMQ
                     RFMERQTQAARQTMQAASSAATAASAHAQTVEKNARAHERMAREVEQTRLRVDALNQK
                     MREEQAQARAAQLGVSSAAEVYIRKMERAGKATHSLGLKSAAARRELGVLISQMARGN
                     FGALRGSGITLANRAGWTGALMSPKGMMTGGVIGGLVAAVLGLGKAWHDGRKEGEKFN
                     RQLALTGHYAGVTVGQLWKLSRAISGNGITQHAAAGALAQVVGSGAFHGNDIGMVAKA
                     AAQMERSVGQSVSDTINQFKRLKDDPVNAAKALDNALHFLNATQLEQIRVLGEQGRSS
                     DAARIAMSALAEETGKRTSDIDNNLNALGSTLQTLSDWWKQFWDAAMNIGREDSLDAQ
                     IDALQEKIQRAKKYPWTNASTQVEYDQQRLNDLQEKKRRKDLQDAKAQAERNYQEQQK
                     RRNAENAALNRMNETEAARHQREIARINAMQYADQAVRDAAIQRENERYEKAIKKNTR
                     ATRNDEATRLLLQYSQQQAQVEGQIAAARQSAGIATERMTEAHKQLLALQQRISDLDG
                     KKLTADEKSVLARKNELIQALTLLDVKQQELQKQTALNDLRKKTVQLTSQLADKERAL
                     REQHNLDIATAGMGDKQRQRYQAQLRIRQEYRQQLQQLENDSRQKGTYGTEDYRRAEE
                     VLKGSLKRQLNENKRYWQELEVAQGDWKNGAMRAFQNFTADADNAAGTAEQMFTVAFS
                     SAGNALATFCTTGKLNFKSFTSSLLSDMARIMAQMAMMQAVKGVGSLFGFTTNADGGV
                     YQSADLSRYSGTVVNRPTFFAFAKGAGVMGEAGPEAILPLRRGADGKLGVVADTGGSG
                     MVMFAPQYNIEINNDGTNGQIGPAALKVVYDLGKKAAADFMQQQARDGGRLSGAYR"
     gene            4289..4618
                     /locus_tag="OO349_003341"
     CDS             4289..4618
                     /locus_tag="OO349_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5868300.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVTIRVRKGEHPSLKAFLERHGGVRAFQWTPPYDWKLIRVVCRKWSASVGALWVTVTA
                     DFEQVVN"
     gene            4618..5316
                     /locus_tag="OO349_003342"
     CDS             4618..5316
                     /locus_tag="OO349_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HCR5868301.1"
                     /translation="MQDIRQETLNECTRAEQSARVELWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGSGFELNGRGCAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLATPTETDG
                     AVFPGRIMLANTCMWTYRSDECGYTGRAVADEFDKPTTDIRKDKCSKCMRGCELRNNT
                     GNFGGFLSINKLSQ"
     gene            5321..6064
                     /locus_tag="OO349_003343"
     CDS             5321..6064
                     /locus_tag="OO349_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5868302.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPEGDRYLPSENISGEPE
                     ERFRMAPEDWLRAQMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHREWHASAFTGICNDLAAASTFV"
     gene            6001..6603
                     /locus_tag="OO349_003344"
     CDS             6001..6603
                     /locus_tag="OO349_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HCR5868303.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLSD
                     GWYQVRIAGRDAGETELSARLNEPLANGAVIHIVPRLVGAKSGGVFQAVLGAAVMAVA
                     IWMPGVGIMASNLLFSLGASMTLGGVAQMLAPKARTPRTQTTDNGKQNTCFSSLDNMV
                     AQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            6664..10144
                     /locus_tag="OO349_003345"
                     /pseudo
     CDS             6664..10144
                     /locus_tag="OO349_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
     gene            10212..10811
                     /locus_tag="OO349_003346"
     CDS             10212..10811
                     /locus_tag="OO349_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HCR5868304.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHQSTLSAGYLHASTNAPG
                     SDDLNGINVKYRYEFTDTLGLVTSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     LSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDNRHSNTSLAW
                     GAGVQFNPTESVAIDLAYEGSGSGDWRTDGFIVGVGYKF"
     gene            10875..>11419
                     /locus_tag="OO349_003347"
     CDS             10875..>11419
                     /locus_tag="OO349_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004981847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCR5868305.1"
                     /translation="MAVKISGVLKDGAGKPVVNCAIELRARRTSPTVVAHVVATCVTD
                     NNGAYVIEAEPGYYEVALHCNGWQPTRVGDIDVAPTDAPGTLNAFLNAPKDGDLRPEV
                     MKRFEEMVAQAQQSAGAAAGNAQQTAQDVAAAATARDDAQRFAEKARQDATVTAEDRK
                     ATAEDVTSTGANAAAAGQSAQ"
BASE COUNT         2681 a         2692 c         3678 g         2368 t
ORIGIN      
        1 tgggcgatgg cggaaatcac gtaccagatc acgtacacga attaaggagg tggtaatgac
       61 cacaccaaat ccactggcaa agacgaaagg tgcggggacg acgttctgga tgtataccgg
      121 caacggcgat gcgtttgcga accctttgtc ggacactgac tggctgcgtc ttgcgatggt
      181 gaaggatctg caacctggcg aaatgaccgc tgatgcagaa gatgacactt atctcgatga
      241 tgaagatgca gactggaaaa cgacaaccca ggggcagaaa tccgttggtg atacttcggc
      301 gacgctggcc tggcgtccgg gtgacagcgg gcagaaaaaa ctggttcagt tgttcgactc
      361 cggtgaagtc tgcgcgtttc gtatcaaata tcccaacggc actgttgatg ttttccgcgg
      421 ctggctgagc tcactgggta aaaccattgc ctcaaaagac gtgatgaccc gcactgtgaa
      481 aatcagcggt gtggggcgtc cgtatctggc agaggaaggc actgaaaccg tgggtgttac
      541 cggactgacg gtggcaccgg catctgtcag tgtcaaagcg ggagcaacca ccacactgac
      601 ctttacagta aaacctgacg gggccagtga caaagcgatc agtgtgcatt cgtcagatcc
      661 tcagactgct tcggtgaccc tgagcgggct tgtggccacg gtgaaaggcg tgaagcaggg
      721 cagtgtcagc atcgtgggca tgacctctga cggagagttt gtggcagtgg ctgcggtgac
      781 cgtcagcgca ccataacagg acgatactca tcattgcccc ggttatccgg ggctttttgc
      841 atccggagaa catgatgttt ctgaaacagg atacgtttaa ttatgaaaaa cagtccgtgg
      901 tgctcagtga gctgtccggg ctgcagagaa ttgaatatct gacgtttgtt cagcagcgaa
      961 cggcaaagtt tgatgcacag gagggagaac tgccggaggc tgaacgacag attgcttttc
     1021 tgcgtatggg aatggatatc aatgcctggc tggtttcccg ctcactgtgg aatgctgagc
     1081 agtctcagga tgttgagacg ctttgcgcat ccattatgac aacatggtcg tatgatgcgc
     1141 tgggcgcggg ggcggagagg gttctgtcgc tgagcggtat ggggaccatt gagaatgccg
     1201 gggatgatga tcatgaggcg ctgacgccgg aaaagtcctg acgcgggaaa tgcagtttgt
     1261 catgcggctt gcccgggagt tccggcgggc agactggcgg cggatgctgt cggatatgtc
     1321 ggccactgag cttggtgagt ggggcgatta tttccggatg cagagcttca gtgatgtgtg
     1381 gatggatgcg cagtttgcct cgctgaaggc attgatcgtg agaatggtgt ccggcagcag
     1441 cgatgctgcg gtggctgatt tcagcctttt accggaagag aacgggatac cggagcgaac
     1501 ggacgaagaa ctgatgcatc ttggggaagg tatttccgga ggtgtgcgtt atggaccaga
     1561 tagccaacct ggtcattgat ttggggattg atgcggcaga gtttaaaaat gaaattcccc
     1621 gtatcaaaaa ccttctgaat ggtgcagcca gcgatgcaga acggtcttct gcccgtatgc
     1681 agcgttttat ggagcgtcag actcaggcgg cccggcagac aatgcaggcg gcttcttcgg
     1741 ctgcaacagc agcatcagcc catgcgcaga cggtggagaa gaatgcacgg gctcatgaac
     1801 gcatggcccg tgaggtggaa caaacccgtc tgcgcgtgga tgccctgaat cagaaaatgc
     1861 gcgaggaaca ggcgcaggcc agggcggctc agctgggggt aagcagtgct gcagaagtgt
     1921 atatccgcaa aatggagcgg gcaggaaaag ccacacattc gctggggctg aaaagtgcgg
     1981 cagcccggcg ggagctgggg gtgttaatca gtcagatggc gcgcggcaat tttggtgcgc
     2041 tgaggggatc cgggataacg ctggctaacc gtgccggatg gacaggcgca ctgatgtcgc
     2101 cgaaaggcat gatgactggc ggcgttattg gcggacttgt cgcggcggtc ctgggtctgg
     2161 gtaaagcctg gcatgacggc cggaaggagg gcgagaaatt taaccgtcag ctggcgctga
     2221 cgggacatta tgccggtgtc actgtcgggc agttgtggaa actcagccgg gccatatccg
     2281 ggaatggtat cacgcaacat gcggcagccg gtgcgctggc acaggtggtg ggcagtgggg
     2341 catttcatgg aaacgatatt ggtatggtgg cgaaagctgc cgcacagatg gagcgatcgg
     2401 ttggccagtc ggtcagcgat accataaatc agtttaagcg gctgaaggat gatcctgtaa
     2461 atgccgcgaa ggcgctggac aatgcgctgc attttttgaa tgccactcag cttgagcaga
     2521 tacgcgtcct tggggagcag gggcggtcca gtgatgcggc ccggatcgcc atgtctgcgc
     2581 tggcagagga aaccggtaaa cgcacatctg atattgataa taatctcaat gcgctgggta
     2641 gtacgctgca aaccttgtct gactggtgga agcagttctg ggatgcggcc atgaatattg
     2701 gtcgtgaaga ctcgctggat gcgcagattg atgcgttaca ggaaaaaatt cagcgcgcga
     2761 aaaaatatcc gtggacaaac gcctccacac aggtggagta cgatcagcag cgtcttaacg
     2821 atcttcagga gaaaaaacgc cggaaggatt tgcaggatgc aaaagcgcag gcagaacgga
     2881 attaccagga gcaacagaaa cgccggaatg ctgaaaatgc cgcgctgaac cggatgaatg
     2941 aaacggaagc tgcacgacat cagcgggaaa ttgcgcgtat taatgccatg cagtacgccg
     3001 accaggctgt cagggatgcg gcgatacagc gtgaaaatga acgttacgaa aaagccatta
     3061 agaaaaatac acgggcaacc cgtaatgatg aggccacccg gttattgctg cagtacagtc
     3121 agcagcaggc acaggtggaa gggcagattg ctgctgccag acagtcagca ggcattgcca
     3181 ctgaaaggat gactgaagcg cataaacagc ttctggccct gcagcagcgc atcagcgacc
     3241 tggacgggaa aaagctgacg gcagatgaaa agagtgtgct ggcccgtaag aatgagctga
     3301 ttcaggcgct gacgctgctg gatgtgaaac agcaggagct gcagaaacag acagcgctta
     3361 acgacctgag aaaaaaaacg gttcagctga ccagccagct ggcagacaaa gaacgtgcac
     3421 tgcgtgagca gcacaatctg gatattgcca ctgcaggtat gggggataag cagcggcagc
     3481 gctaccaggc acagttgcgc atccggcagg aataccggca acagttgcaa cagcttgaga
     3541 atgacagtcg ccagaaaggc acttacggga cggaggacta ccggagggct gaggaggtgc
     3601 tgaaggggag cctgaagcga caactgaatg aaaacaaacg ctactggcag gaactggaag
     3661 tggcgcaggg cgactggaaa aacggtgcca tgcgggcgtt tcagaatttt acggcggatg
     3721 cggataatgc ggcgggaacg gcagaacaga tgtttacagt ggcattcagc agtgccggta
     3781 atgcactggc gacattctgt accaccggta agctgaattt taaatccttc acctcttccc
     3841 tgttgtcaga tatggccaga attatggcac agatggccat gatgcaggcg gtaaagggcg
     3901 tcggttcttt attcggcttc acgactaatg ctgatggcgg tgtttaccag tctgctgatt
     3961 tgagtcgcta cagtggcacg gtggttaacc gtccgacgtt ttttgctttt gcaaaaggcg
     4021 cgggtgtgat gggggaagcg ggacctgaag ccattctgcc actgcgtcgc ggtgctgatg
     4081 gtaagctggg ggttgtggcg gatactggtg gttcaggcat ggtgatgttt gccccgcagt
     4141 acaacattga gatcaataac gacggcacga acgggcagat aggtccggct gccctgaagg
     4201 tggtttatga cctcgggaaa aaagcggccg cggactttat gcaacagcag gcccgtgatg
     4261 gtggccggtt aagtggagca taccggtaat ggagacgttt cactggaaag tgcgcccgga
     4321 tatgaatgtg gtatcagagc cgaaagtggt gacagtgaag ctgggcgatg gttatgaaca
     4381 gcgtcgtgcg gcgggactga ataaccagtt gtcgacttac agcgtgacga tacgtgttcg
     4441 taaaggtgaa cacccatctt taaaagcctt tctggaacgg cacggtggcg tccgcgcatt
     4501 tcagtggacg ccaccttatg actggaaact gatcagggtg gtttgtcgta aatggtcggc
     4561 aagcgtgggg gcgttatggg tgactgtaac ggccgatttt gaacaggtgg tgaactgatg
     4621 caggatatcc ggcaggaaac actgaatgaa tgcacccgtg cggagcagtc agcccgggtg
     4681 gagctctggg aaatcgatct gacagaggtc ggtggtgagc gttatttttt ctgtaatgag
     4741 cagaacgaaa aaggtgagcc ggtcacctgg caggggcggc agtatcaggc ataccccatt
     4801 caggggtcgg ggtttgaact gaacggcagg ggctgtgctg cccgtccgac actgacggtc
     4861 tctaacctgc acggcatggt caccgggatg gcggaagatc tgcagagtct ggtcggcgga
     4921 acggtggtcc ggcgtaaggt ttacgcccgt tttctggatg cggtgaactt cgtcaacgga
     4981 aacagcgacg ccgatccgga acaggaggtg atcagccgct ggcgcatcga gcagtgcagc
     5041 gaactgagcg cggtcagtgc ctcttttgtg ctggccacac caacggagac ggatggcgcg
     5101 gttttcccgg ggcgtatcat gctggcgaat acctgtatgt ggacttaccg ttctgatgag
     5161 tgtggttaca cgggcagggc agtggctgac gagttcgaca aaccaacaac ggatatccgg
     5221 aaggacaaat gcagcaagtg tatgcgcggg tgtgagttgc gcaacaatac cggtaatttc
     5281 ggcggtttcc tttccatcaa taaactttct cagtaaatcc atgacacaga cagaatcagc
     5341 gattctggcg cacgcccggc gatgtgcgcc agcggagtcg tgtggcttcg tggtgagaac
     5401 gccggaggga gacaggtatc ttcccagcga gaatatctcc ggtgagccgg aggaacggtt
     5461 ccggatggct ccggaggact ggctgcgggc acaaatgcag ggtgagattg tggcactggt
     5521 ccacagtcat cccggtggtc tgccctggct gagtgaggct gaccggcggc tgcaggtgca
     5581 gagtgatttg ccgtggtggc tggtctgccg gggggcgatt cacaagttcc gctgtgtgcc
     5641 gcatctcacc gggcggcgct ttgagcacgg ggtgacggac tgttacacgc tgttccggga
     5701 tgcttatcat ctggcgggga ttgagatgcc ggattttcat cgcggggatg actggtggcg
     5761 tcacggtcag aatctctatc tggataatct ggaggccaca gggctgtatc aggtgccgtt
     5821 gtcatcagca caaccgggcg atgtgctgct gtgctgtttt ggttcatcgg tgccgaatca
     5881 tgccgccatt tactgcggcg atggtgagct gctgcaccat attcctgaac aactgagtaa
     5941 acgagagagg tataccgaca aatggcagcg acgcacacac tccctctggc gtcaccggga
     6001 atggcacgca tctgccttta cggggatttg caacgatttg gccgccgcat cgaccttcgt
     6061 gtgaaaacgg gggctgaagc catccgcgca ctggccacac agctcccggc gtttcgtcag
     6121 aaactgagtg acggctggta tcaggtgcgc attgccgggc gtgatgcagg tgaaaccgaa
     6181 ttgtctgccc gtcttaatga gccgctggca aatggtgcag tgatccacat agtaccgcgt
     6241 ctggtgggag ctaaaagtgg cggtgtgttt caggcggtgc tgggggcagc tgttatggcg
     6301 gttgctatat ggatgccggg ggtaggaatt atggcgagta atctgctgtt ttctctcggt
     6361 gccagtatga cgcttggcgg tgttgcacag atgctggcac cgaaagccag aactccccgt
     6421 acacagacaa cggataacgg caaacagaac acctgtttct cctcactgga taacatggtt
     6481 gcccagggca atgttctgcc tgttctgtac ggtgaaatgc gcgtggggtc gcgggtggta
     6541 tctcaggaga tcagcacggc agacgaaggg gatggtggtc aggttgtggt gattggtcgc
     6601 tgatgcaaaa tgtttcatgt gaaaccgcct gcgggcggtt ttgtcgttta tggagcgtga
     6661 ggaatgggta aaggcagcag taaggggcat accccgcgcg aagcgaagga caacctgaaa
     6721 tccacgcagt tgctgagtgt gattgatgcc atcagcgaag ggccggttga cggtccggtg
     6781 gatggattaa aaagcgtgct gctgaacggt acgccggtcc tggatagcga ggggaagaca
     6841 aacttttccg gtgttacggt ggtgttccgc gccggcgagc aggagcagac accgccggag
     6901 gggtttgaat cttccggctc agagactgtg ctgggtacgg aagtgaaata cgacacgccg
     6961 atcacccgga ccatcacgtc ggcaaacatt gatcgtctgc gcctgacctt cggtgtgcag
     7021 gcactggtgg aaaccacctc aaagggggac cggaatccgt cggaagtccg cctgctggtt
     7081 cagatacagc gtaacggtgg ctgggtgacg gaaaaagaca tcaccattaa gggcaaaacc
     7141 acttcgcagt atctggcctc ggtggtggtg ggtaacctgc cgccgcgccc gtttaatatc
     7201 cggatgcgca ggatgacacc ggacagcacc acagaccagc tgcagaacaa aacgctctgg
     7261 tcgtcgtaca ccgaaatcat cgatgtgaaa cagtgctacc cgaacacggc gctggtcggc
     7321 gtgcaggtgg attcagagca gttcggtaac cagcaggtga gtcgcaatta tcatcttcgc
     7381 gggcgcattc tgcaggtgcc gtcgaactat aacccgcaga cgcggcaata cagcggtatc
     7441 tgggacggaa cgcttaagcc agcatacagc aacaacatgg cctggtgtct gtgggacatg
     7501 ctcactcatc cgcgctacgg catggggaaa cgtcttggtg cggcagatgt ggacaaatgg
     7561 gcgctgtatg tcatcggcca gtactgcgac cagtcagtgc cggacggatt tggcggcacg
     7621 gagccgcgca tcacctgtaa cgcttacctg accacacagc gtaaggcgtg ggatgttctc
     7681 agtgatttct gctcggcgat gcgctgtatg ccggtatgga acgggcagac gctgacgttc
     7741 gtgcaggacc gaccgtcgga taaagtgtgg acctataacc gcagtaatgt ggtgatgccg
     7801 gatgatggcg cgccgttccg ctacagcttc agcgccctga aggaccgcca taatgtcgtt
     7861 gaggtgaact ggattgatcc ggacaacggt catgagacgg cgacagagct tgtggaggac
     7921 acgcaggcca ttgtccgtta cggtcgtaac gtcacgaaga tggatgcctt tggctgtacc
     7981 agccggggac aggcgcaccg cgccgggctg tggctgatta aaacggaact gctggaaacg
     8041 cagaccgtgg acttcagcgt cggcgcagaa gggcttcgcc atgtaccggg tgatgtcatt
     8101 gaaatctgcg atgatgatta tgccggtatc agcaccggtg gtcgcgtact ggcggtgaac
     8161 agccagaccc ggacgctgac gctcgaccgt gaaatcacgc tgccgtcctc cggtaccacg
     8221 ctgataagcc tggttgacgg aaatggcaat ccggtcagcg tggaggttca gtccgtcacc
     8281 gacggcgtga aggtgaaagt gagccgggtt cctgacggcg ttgcagaata cagcgtgtgg
     8341 gggctgaagc tgccgacgct gcgccagcgc ctgttccgct gcgtgagtat ccgtgagaac
     8401 gacgacggca cgtatgccat caccgccgtg cagcatgtgc cggaaaaaga ggccatcgtg
     8461 gataacgggg cacactttga cggtgaccgg cgcggcacgg tgaatggtgt cacgccgcca
     8521 gcggtgcagc atctgaccgc cgaagtcacc gcagacagcg gggaatatca ggtgctggcg
     8581 cgatgggaca cgccgaaggt ggtgaagggt gtgagcttcc tgcttcgcct gaccgtggca
     8641 gcggacgacg gcagtgagcg gctggtcagc acagccagga cgacggaaac cacataccgc
     8701 ttcaggcagc tggcgctggg gaattacagt ctgacagtcc gggcggtaaa tgcccggggg
     8761 cagcagggcg atccggcgtc ggtatcgttc cggattgcgg caccggcagc gcctgtcact
     8821 attgaactga taccggggta ttttcagata acggtggtcc cgaaactggc tgtatatgac
     8881 ccgacggtgc agtttgagtt ctggttttcg gaaaagcgga ttgcggatat caggcaggtt
     8941 gaaaccagcg cccgctatct tggcacggcg ctgtactgga tagccgccag tatcaatatc
     9001 aggccgggcc atgattatta tttttacgtt cgcagtgtga acaccgttgg caaatcggca
     9061 tttgtggagg ccgtcggtcg ggcgagcgat gatgcggaag gttatctgag tttttataaa
     9121 gggttgatca ataaaacgca tctcggcaag gagttgtgga cgcagattga taacggtcag
     9181 cttgcgccgg acctgactga aatcaggacg tccattacga atgtcagcaa tgaaatcacg
     9241 caaaccgtca ataaaaaact ggaaaatcag agtgctgcaa tccagcagat acagaaagtt
     9301 caggttgata caaataataa cctgaacagc atgtgggctg tgaagctgca acagatgaag
     9361 gacggacgcc tttatattgc gtggtatcgg tgccggtatt gagaatacgc cagcaggaat
     9421 gcagagtcag gtgctgctgg cggcagacag gattgcgatg attaatcctg cgaatggcaa
     9481 cacaaagccg atgtttgttg gtcagggcga tcagatattc atgaacgacg tgttcctgaa
     9541 acgcctgacg gctccgacca ttaccagcgg cggtaatcct ccggcatttt ccctgacacc
     9601 tggcggacgg ctgacggcga aaaatgccga tatcagcggt aacgtgaacg cgaactccgg
     9661 gacgctcaac aacgtcacga ttaataagaa ctgtcgggtt ctgggaaaat tgtccgcgaa
     9721 ccagattgaa ggcgatctcg ttaaaacagt gggcaaacct ttcccacggg actcccgggc
     9781 accggagagg tggccatcag ggaccattac cgtcagggtt tatgacgatc agccgtttga
     9841 tcggcaaatt gttattcccg cggtggcgtt tcgcggtgct aaacatgagc ggaagaataa
     9901 caatatttat tcgtcatgcc gcctgatagt gaagaaaaac ggtgctgaaa tttataaccg
     9961 aacgaccctg gataatacgc tgatatatac gggtgttatt gatatgcctg ccggtcacgg
    10021 tcacatgacg ctggagtttt ctgtatcggc atggctggta aatggctggt atcccacagc
    10081 aagtatcagc gatttgctgg ttgttgtgat gaagaaatcc acagcaggta tcagtatcag
    10141 ctgaatttta taacccagat acgggcacca gaaatggtgc cttttttatt gcagaaaagc
    10201 gagaggtaat tatgcgtaaa ctttatgctg ccattttgtc cgcagccatc tgtctggctg
    10261 tatccggtgc gcctgcatgg gcgtctgaac atcagtccac gctgagcgcg gggtatcttc
    10321 atgcctcgac gaacgctccc ggcagtgatg atctgaacgg gattaacgtg aaataccgtt
    10381 atgagtttac ggacacgctg gggctggtga cgtcattcag ctatgccaac gctgaagatg
    10441 agcaaaaaac gcattacagt gatacccgct ggcatgagga ttccgtgcgt aaccgctggt
    10501 tcagcgtgat ggcggggctg tctgtgcgcg tgaatgaatg gttcagcgcg tatgcgatgg
    10561 cgggcgtggc ttacagccgt gtgtcgacct tctccgggga ttatctccgc gtaactgaca
    10621 acaaggggaa aacgcacgat gtgctgaccg ggagtgatga caaccgccac agcaacacgt
    10681 ctctggcgtg gggggctggc gtgcagttta acccgaccga atccgtggcc attgaccttg
    10741 cttatgaagg ttccggcagt ggcgactggc gcactgacgg tttcatcgtg ggtgtcggtt
    10801 ataagttctg attagccagg taacacagtg ttatgacagc ccgctggttc aggcgggctt
    10861 tttgtggggt gaatatggca gtaaagattt caggtgtact gaaagatggg gcgggtaaac
    10921 ctgtcgtaaa ttgtgcgatt gaactgcggg ccagaagaac cagtccgacc gttgtggcac
    10981 acgttgttgc cacttgcgtg acggacaata acggtgctta tgtgattgag gctgagccgg
    11041 ggtattacga ggttgcgctt cactgtaacg gctggcagcc aacccgtgtc ggggatattg
    11101 atgtggcacc gactgatgca ccggggacac tgaacgcgtt tctgaatgca ccaaaggatg
    11161 gtgatttacg tccggaggtg atgaagcgct ttgaggaaat ggtggcgcag gcgcagcaga
    11221 gtgccggggc tgcagccgga aacgcacagc agacggcgca ggatgtggcg gcagccgcaa
    11281 cggcccgtga tgatgcacaa cgttttgcgg agaaagcccg acaggatgca accgtcacag
    11341 ctgaggacag aaaggccact gcggaagatg tgacaagcac aggagcaaat gcagccgcag
    11401 ccggacagag cgcacagga
//
LOCUS       DAJTQY010000113        11370 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.113, whole genome shotgun sequence.
ACCESSION   DAJTQY010000113 DAJTQY010000000
VERSION     DAJTQY010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11370
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.113"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            404..2044
                     /gene="trg"
                     /locus_tag="OO349_003348"
     CDS             404..2044
                     /gene="trg"
                     /locus_tag="OO349_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HCR5868306.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEAFDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVNVFRLNKHSVSAEPRGAGEPVSFATV"
     gene            complement(2082..3005)
                     /locus_tag="OO349_003349"
     CDS             complement(2082..3005)
                     /locus_tag="OO349_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5868307.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSN
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSTSLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            3222..4565
                     /locus_tag="OO349_003350"
     CDS             3222..4565
                     /locus_tag="OO349_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR5868308.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQLATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMTPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            4790..6445
                     /gene="opgD"
                     /locus_tag="OO349_003351"
                     /gene_synonym="mdoD"
     CDS             4790..6445
                     /gene="opgD"
                     /locus_tag="OO349_003351"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HCR5868309.1"
                     /translation="MDRRRFIKGLMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGISP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            6585..6809
                     /locus_tag="OO349_003352"
     CDS             6585..6809
                     /locus_tag="OO349_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HCR5868310.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            6872..7408
                     /gene="rimL"
                     /locus_tag="OO349_003353"
     CDS             6872..7408
                     /gene="rimL"
                     /locus_tag="OO349_003353"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HCR5868311.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(7403..8383)
                     /locus_tag="OO349_003354"
     CDS             complement(7403..8383)
                     /locus_tag="OO349_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5868312.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVYVQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            8507..9499
                     /gene="tehA"
                     /locus_tag="OO349_003355"
     CDS             8507..9499
                     /gene="tehA"
                     /locus_tag="OO349_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HCR5868313.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCMFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            9496..10089
                     /gene="tehB"
                     /locus_tag="OO349_003356"
     CDS             9496..10089
                     /gene="tehB"
                     /locus_tag="OO349_003356"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HCR5868314.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFTFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            10392..11060
                     /locus_tag="OO349_003357"
     CDS             10392..11060
                     /locus_tag="OO349_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HCR5868315.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATGKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMK
                     EAIAQRKPVVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTM
                     DTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFD
                     VNKK"
     gene            complement(11078..11356)
                     /gene="tnpA"
                     /locus_tag="OO349_003358"
                     /pseudo
     CDS             complement(11078..11356)
                     /gene="tnpA"
                     /locus_tag="OO349_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311304.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT         2931 a         2652 c         2858 g         2929 t
ORIGIN      
        1 tttcaaaagg gtattttggc tttgacatat taggggctat tccatttcat cgtccaacaa
       61 aatgggtgca gtacagttga tagaaatatc ggtcggggcc ttcgtctttc tgattcccgg
      121 ttagcctgaa aacagaaagt ctcaggcacc cgcaggcatc ctatgaggtt tccttaggga
      181 cgaaaataat cacttcacga aattgcgtgc tgttttccag aatttttcgt cattcgggtt
      241 agccagttta gccattcgtt actctcttca ttccaatagc attaattttc tctgcaataa
      301 ttgttgtaaa aatgtgacgc aaagaggttt ttggtcataa gtaattaccg tcaagtgccg
      361 atgactttct atcaggagta aacctggacg agagacaacg gtaatgaata caactccctc
      421 acagcgatta ggttttttgc atcacatcag gctggttccg ttatttgcct gcattcttgg
      481 cggtatctta gttctattcg cattaagttc tgctctggct ggctatttcc tctggcaggc
      541 cgatcgcgat cagcgtgatg ttactgcgga gattgagatt cggaccgggt tagcgaacag
      601 ttcagatttt ttgcgttcag cccggatcaa tatgattcag gccggggctg cgagtcgtat
      661 tgcggaaatg gaagcaatga agcgaaatat tgcgcaagcc gaatcggaga ttaaacagtc
      721 gcagcaaggt tatcgtgctt atcagaatcg accggtgaaa acacctgctg atgaagcctt
      781 cgacactgaa ttaaatcaac gctttcaggc ttatatcacg ggtatgcaac ctatgttgaa
      841 atatgccaaa aatggcatgt ttgaagcgat tatcaatcat gaaagtgagc agatccgacc
      901 gctggataat gcttataccg atattttgaa caaagccgtt aagatacgta gcaccagagc
      961 caaccaactg gcggaactgg cccatcagcg cacccgcctg ggtgggatgt tcatgattgg
     1021 cgcgtttgtg cttgccctgg tcatgacgct gataacattt atggtgctac gtcggatcgt
     1081 cattcgtcca ctgcaacatg ccgcacaacg gattgaaaaa atcgccagtg gcgatctgac
     1141 gatgaatgat gaaccggcgg gtcgtaatga aatcggtcgc ttaagtcgtc atttacagca
     1201 aatgcagcat tcactgggga tgacagtagg gactgttcga cagggcgcgg aagagattta
     1261 tcgtggcacc agcgaaattt cagctggcaa tgcggacctg tcatctcgca ccgaagaaca
     1321 agcggcggct atcgaacaaa ctgccgccag catggagcaa ctcactgcga cggtgaaaca
     1381 gaatgcggat aacgcgcatc atgccagcaa actggcgcaa gaggcttcta ttaaagccag
     1441 cgatggcggg cagacggttt ccggtgtagt aaaaacgatg ggcgctatct ccacgagttc
     1501 gaagaaaatt tctgagatca ccgccgtcat caacagtatt gctttccaga cgaatattct
     1561 ggcactgaat gctgccgttg aagccgcgcg agcgggtgag caagggcgtg gatttgccgt
     1621 tgtcgccagc gaagtacgga cactcgcaag ccgcagcgct caggcagcga aagagattga
     1681 aggcttgatc agtgaatcag tcaggttaat tgacctgggg tcggatgagg tggcaacggc
     1741 agggaaaacc atgagcacta ttgttgatgc cgtcgcgagt gtcacacata tcatgcagga
     1801 aatcgccgcc gcctcggatg aacaaagtag aggcataact caggttagcc aggcgatttc
     1861 tgaaatggat aaggtgacgc aacagaatgc ttctctggta gaagaggcct cagcagcggc
     1921 ggtgtccctt gaagaacagg ctgcacgatt aactgaggcg gtgaacgtat tccgtctgaa
     1981 caaacattct gtgtcggcag aacctcgcgg agcgggtgaa ccagttagtt tcgctacggt
     2041 gtgaaaatgt tcaaggaggg atcgacagat cccttcacct ttcagaacgg cattgatttt
     2101 cgaatagcgt taatcatcaa ctggcaacca gaagagaacg tcgcatctac gcgggtcagt
     2161 attccaatcg gttcgcctgc accatgtccc ggaacaggca gggccaccag cgtggcatga
     2221 cgcaggtcgt cttttacagc gccagaaggg acaaaccaca cgtaatcgta ttcaacggta
     2281 agttgacgag atagcgaagt agacagcgtt tcgatacaac ccgaaggaat tttacagccc
     2341 tggctctgca ctaatgcatc tgaatgctgg cgtggcgcag tgccttctgg tgatacaacg
     2401 accggccatt ccagcacccg gcttagcgtt acgttctcct gaagtagcgg gtgattaggg
     2461 cggacaacca gcttcaacga ttcaagaaac agcagttcgt aattaagccc ggtcatcagt
     2521 tcaggatctg acatccgacc aatgccgata tcgatttccc cggtttttaa acccgccaga
     2581 atcatagggt tactcattgt cgcaacttgc aaggtcgtct ctttttgttg ctgatgaaac
     2641 tgacctataa ccgaaggtaa tatccccagt gccgcagtag gtagtgcacc aaccctgacg
     2701 acatcattat taagaccttc tttacgatga agcgactgtc cggcagtgtt gatggcgtca
     2761 agaactctga ctgcatgcgt taaaaattgt tcgccgggta aggtaagttg cgccccctga
     2821 cgaccacgct caaacaagcg agcgcccgtc agctgctcca gttcattcaa tgtattagag
     2881 agcgcaggtt gactcaaatt aagggtttca gctgcgcgcc ccaaagttcc ttgttgtgcg
     2941 acagctacga atgtatgaag gtggcgcaaa cggatgcgct gactaaacag actatttttt
     3001 tccataagcg atgttaaaaa cgaagcggtg tcgctgacaa gtgaagttgt ttgattatga
     3061 taacttgatt gcaaaatatt attaataatt aaagcaatta tgttacagca aaatggataa
     3121 tattgatgtt ttcgcggcga gatcacagtt tgtaaattct tcccgccaga gtgaatgcgg
     3181 ttacctacac tccagattac tgaccactgg aggcagacac tatggcgaac agcatcacgg
     3241 cggatgagat tcgggaacag ttttcgcagg caatgtcagc catgtaccag caagaagttc
     3301 cgcaatatgg cacgctgctg gaactggtag ctgatgtgaa tctggctgtg ctggaaaaca
     3361 atcctcaact gcacgaaaaa atggtaaatg cagacgagct ggcgcgactg aatgttgaac
     3421 gtcatggggc gattcgcgtt gggactgcac aagagcttgc tactcttcgg cggatgtttg
     3481 ccattatggg gatgtacccg gtgagctatt acgatctctc gcaggcaggg gtgccggtac
     3541 attcgacagc atttcggccc attgatgatg cttctctggc gcgtaatccc ttccgcgttt
     3601 ttacatcctt actccgcctt gagcttatcg agaacgaaat tttgcgccag aaagcggcgg
     3661 agattctgcg tcagcgcgat atcttcaccc cacgttgtcg acaactgtta gaggaatatg
     3721 agcagcaggg cggttttaac gaaacacagg cacaggagtt tgtgcaggaa gccctggaaa
     3781 cgtttcgctg gcaccagtta gcaacggtag atgaagaaac ctatcgcgcc ttgcacaacg
     3841 aacatcggtt gattgctgat gtggtctgtt ttcctggatg ccatatcaac cacctgacgc
     3901 cacgtacgct ggatattgac cgggtgcagt cgatgacgcc tgaatgcgga attgaaccca
     3961 aaattctcat cgaggggccg ccgcgccgcg aggtaccgat tttactacgc cagaccagct
     4021 ttaaagcact ggaagagacg gtgttgtttg cggggcagaa acagggcacg cataccgcgc
     4081 gctttggtga aattgagcag cgtggcgtgg cattaacgcc gaaagggcga caactgtatg
     4141 atgatcttct gcgtaacgct ggaaccgggc aggataatct cactcaccaa atgcatttac
     4201 aggaaacctt ccgcactttt cctgacagtg agtttttaat gcgtcagcaa gggttggcat
     4261 ggttccggta ccgtctgacg ccttcgggtg aggcgcatcg tcaggcgatt catcctggag
     4321 acgatccaca gcccttaatt gaacgtggtt gggtagtggc gcaacccatc acctatgaag
     4381 atttcttgcc cgttagcgcg gcggggatct tccagtcaaa tctgggtaat gaaacgcaga
     4441 cacgcagtca cggtaatgcc agtcgcgaag catttgagca ggcgttgggt tgtccggttt
     4501 tggatgagtt ccagctttac caggaagcgg aagaacgcag taaacgtcgc tgtggtttgc
     4561 tttaaattct gaccatccgc ctttgcaaaa atttgcctga ttttacaaac gaatcaggct
     4621 catcccatcg acataaaaaa aatgccgatt tatgcatatt ctctcagttc aacaattgga
     4681 ttattaataa atattgtcta gagtgagcgg tcataaataa gcactttctt gccgctgaaa
     4741 acgaccagcg cgggaccatt cacaacacca gaaggactca ctttcaggta tggatcgtag
     4801 acgatttatt aaaggtttaa tggctatggc cgccgtgtgc ggtaccagcg gcattgcttc
     4861 tcttttttct caggcagcat tcgcggcaga ttctgatatt gccgacgggc aaacccagcg
     4921 ttttgacttc tccattctac agtcaatggc gcacgactta gcgcaaacag cgtggcgtgg
     4981 tgcgcctcgt ccgttacctg acacgctggc gacaatgacg ccgcaggctt ataacagtat
     5041 tcaatacgac gccgaaaaat cgctctggca taacgttgag aaccgtcaac tggacgctca
     5101 gttcttccat atgggaatgg gattccgtcg ccgcgttcgt atgttttctg tagatccagc
     5161 aacacatctg gcgcgtgaaa ttcactttcg cccggagttg ttcaaataca acgatgcagg
     5221 tgttgatacc aaacaattag aagggcaaag cgatctcggc tttgccggtt ttcgcgtgtt
     5281 taaagccccc gaactggcgc gccgtgatgt agtatcattt ctcggcgcga gttatttccg
     5341 cgccgttgat gatacatatc aatacggttt gtcggcccgc ggcctggcga tcgacactta
     5401 caccgacagt aaagaagagt tccccgactt taccgccttc tggtttgata cggtaaaacc
     5461 gggggcaact acctttaccg tttatgcgtt gctcgatagc gccagcatta ctggtgccta
     5521 taagttcact atccattgtg agaaaagtca ggtgattatg gatgtggaaa atcacctgta
     5581 tgcgcgcaaa gacattaaac agctgggcat ttcgccgatg accagtatgt tcagctgcgg
     5641 tactaatgaa cgtcggatgt gcgatacaat tcatccgcaa attcatgact ctgatcgtct
     5701 gtccatgtgg cggggcaacg gcgagtggat ttgccgtccg ctgaataatc cgcaaaaatt
     5761 gcagttcaat gcttacaccg acaacaaccc gaaagggttt ggtttattgc aactggatcg
     5821 tgacttctcc cattatcagg acattatggg ctggtataac aaacgcccaa gtctgtgggt
     5881 ggaaccgcgt aacaagtggg gtaagggcac catcggcctg atggaaatcc caacaacggg
     5941 cgaaacgctg gataacattg tctgcttctg gcagccagaa aaagctgtaa aagcaggtga
     6001 tgagtttgca ttccagtatc gtctgtactg gagtgcgcaa ccgcctgttc attgcccatt
     6061 agcgcgcgtt atggcgacgc gtaccggcat gggcggtttc ccggaaggtt gggcgccagg
     6121 tgaacactat cccgaaaaat gggcgcgtcg ttttgccgtc gatttcgttg gtggtgatct
     6181 gaaagctgcc gcgccaaaag gcattgagcc ggtgattacg ctttccagtg gggaagcgaa
     6241 gcaaatcgaa attctctata ttgaacccat cgatggttat cgtattcagt ttgactggta
     6301 tccgacttcg gactccactg atccggtcga tatgcggatg tatctacgtt gtcaggggga
     6361 cgctatcagt gaaacatggc tgtatcagta tttcccgcca gcgccggata aacgtcagta
     6421 tgttgacgac cgcgtgatga gttaatcgtt ttttcttcgg caccttcttc gggaggtgcc
     6481 gtctggttaa acacgatccc gctcgcattt ttccctaagt taaatgagta atctgatggt
     6541 gtgtatttca gatacaccgt gtcagccact aacagggagt gcgtatgttt ccagaatacc
     6601 gagatttaat atcccgtctg aaaaacgaaa atcctcgctt tatgtccttg ttcgataaac
     6661 acaataaact tgatcatgaa attgccagaa aggaaggttc cgacggtcga gggtacaatg
     6721 cggaagtggt ccgcatgaaa aaacaaaagc tacagttaaa agatgagatg ctcaaaatcc
     6781 tgcagcagga gagcgtcaaa gaggtgtaaa ctttcctaag ccgccagcca ggcggctttt
     6841 ttaacaacag catggattga ctggagataa gatgactgaa acgataaaag taagcgaatc
     6901 acttgaatta catgctgttg cggaaagtca cgtcacacct ctttatcagt taatctgtaa
     6961 aaataaaacc tggttacagc agtcgctaaa ctggccgcag ttcgttcaga gtgaagagga
     7021 cacgcgaaaa acggtgcagg gtaatgtgat gttgcatcaa cgcggctatg ccaaaatgtt
     7081 catgattttc aaagaagatg aacttatcgg cgttatctcg tttaatcgta ttgaaccact
     7141 gaataaaact gctgaaatcg gctactggct ggacgaatct catcaggggc aggggatcat
     7201 ttctcaggcg ctgcaggcat tgattcatca ttacgctcag tctggtgaac ttagacgctt
     7261 cgtgatcaaa tgtcgggtgg acaatccgca aagcaaccag gtcgctttgc gcaatggttt
     7321 tatccttgaa ggttgcctga aacaggctga gttcctgaat gatgcctatg atgatgtgaa
     7381 cttatacgcg cgtattatcg attcataata accctgccag cggcgttcgc gtaatgcgct
     7441 cctcgccgtt aatgactttt ggaccacgga catgaaccgt gtcgccatca aaggcttcaa
     7501 cgacggcgtg gtcggttaac tccacatggt tttcaattat caccgcacct gtaatacggc
     7561 tctcaccttg tattacaacg tgctcgtcga gcagaatcgg tccaccacgt accacggcat
     7621 ttccgccaat caagacatga tgttttaaca cacaattacc ttccacaatg gcatattccg
     7681 ccacctgcga actgtaatga atcgtcggaa tggcatcttc ttctatgcca gctttcacct
     7741 gcgcgtgacc gtagacttta gcgcaatcgc atagccagac gttgttctct tcattacctt
     7801 caacactggc aaaatcaaaa acttcggcgc gatgttcaat aaaagcatac cggacgacgg
     7861 catcgccata aatttgtgcc tggtggacaa tacgcgaggc gctaaccctt gcgcgatcat
     7921 aaatttggag caggagttga tggtcgggcg ttaagccttg tgcggcgacg atcatagaat
     7981 gttgatcaat taaggcgtga ccaaatattc tacattgcac gtaaactaat gagtcatgaa
     8041 tggtgacact gtcacttata tatgcaccct ggctgatttc actattatcg atccagacgt
     8101 tatccgttgc gtaaacctct ccccacagca cgctggtgcc ggtgatacgc gtattaccgg
     8161 aaatcaccgt tccgccgaac gcaatagcat tctggtcgta aatccagcaa tttccttcct
     8221 gtgctaacac cgtttcgcga tcgatccagc cgccagcggt tcctgctatc acatcgttaa
     8281 aatcgctaat ggcgataatc tgccgtaata acacactttt tttagtgcca tcttcctgat
     8341 aactaaaggc tcgctgttct tcacttaggc gatatttgcg catagcgttt tcccacaggt
     8401 gacttactat aaccgtagca aattctgcgg ctctggctat gctcgagaaa attccataaa
     8461 atgcatttca aatatacttt ataaattaaa caaaatgagt aagaagatgc agagcgataa
     8521 agtgctcaat ttgccggcag gctactttgg tattgtgttg gggacgatag ggatgggatt
     8581 tgcctggcgc tatgccagcc aggtttggca ggtcagccac tggttagggg atgggctggt
     8641 gattctggcg atgatcatct ggggattatt gactagcgca tttattaccc gactcatacg
     8701 ctttccgcat agcgtgctgg cggaagttcg ccatccagtg ctgagcagtt ttgtgagttt
     8761 gtttccggca acgacgatgc tggtggcgat tggttttgtt ccgtggtttc gcccactggc
     8821 ggtgtgcatg ttcagttttg gtgtcgtggt tcagttggct tatgccgcct ggcaaactgc
     8881 gggattatgg cgcggatctc accctgaaga agctaccacg cctggactgt atctgccgac
     8941 agttgccaac aactttatca gcgcaatggc ctgtggtgcg ttgggctaca ccgacgccgg
     9001 tctggtgttt ttaggcgcag gcgttttctc atggctaagc cttgaaccgg tgatcttgca
     9061 gcgtctgcgc agttcgggag aattacccac ggcactgcgg acatcactcg gcattcagct
     9121 cgctcctgcg ctggtggcct gtagtgcctg gctgagcgtc aacggcggcg agggtgacac
     9181 gctggcgaaa atgcttttcg gttatggact gctgcaactg ctgtttatgc tacgtctgat
     9241 gccatggtat ctctcccagc catttaatgc ttcattctgg agtttctcgt tcggcgtatc
     9301 tgcactggca accaccggtt tgcatctggg gagtggcagc gataatggat ttttccatac
     9361 gctggcggtg ccgctgttta tctttaccaa ttttattatt gcaatactgc tcatccgtac
     9421 ttttgcgctt ctgatgcagg gaaaattgtt agtcagaacc gagcgcgccg ttttaatgaa
     9481 agcagaggac aaagaatgat cattcgtgac gaaaactatt ttactgataa atatgaatta
     9541 acccgcacac actctgaagt actggaagcg gtgaaagtgg ttaaaccggg taaaacgctg
     9601 gatctgggct gtggcaatgg tcgtaacagt ctttacctgg cagccaatgg ttatgatgtt
     9661 gacgcatggg ataaaaatgc catgagtatc gccaacgtcg agcgcattaa atccattgaa
     9721 aatctggata atttacacac ccgggtcgtt gatctgaata acctcacatt tgatggacag
     9781 tacgatttta ttctttcgac tgtggtgctg atgttccttg aggctaaaac catcccaggg
     9841 ttgattgcca atatgcaacg ttgcactaaa cctggtggtt acaacctgat tgtggcggcg
     9901 atggataccg ctgattatcc atgtaccgtc ggcttcacgt ttgccttcaa agagggagaa
     9961 ttacgtcgat attacgaagg ctgggagagg gtgaaataca atgaagacgt cggcgagctg
    10021 caccgcaccg acgccaacgg taatcgtatt aaactgcgtt tcgccacgat gctggcacgt
    10081 aaaaaatgac ccggtaagca caaaacgcgt gaaaattccc cacgctgaga tgatttactg
    10141 ttcttctttt cggtaagcat attttttatc gaagggatgt gaaattaatc acagtagtcg
    10201 aagtttttta gcagcttaac ttactgaaat ttaactactg atgattgact tagccccttt
    10261 ttcggcattg actatgtcgt ctgaaaaggg gctgaaaaat ttattttcac caacactttt
    10321 tttgccacaa cacgaagcgg cgctttttgc tataacttag aaagtaatat aatcatctca
    10381 ggaaactatt catgcgtacc acatcatttg cgaaagttgc agctttatgc ggcttgttgg
    10441 ctctgtctgg ttgtgcctct aaaatcaccc agccagataa atattctggt tttttaaaca
    10501 attactctga tttaaaagaa acaacctcag caacaggtaa acctgtttta cgttgggtag
    10561 acccgagttt tgatcaaagc aaatatgaca gcatcgtctg gaacccaatc acttattatc
    10621 cggtaccaaa accttctacc caggtagggc agaaagttct ggataaaatt ttgaactata
    10681 ccaacaccga aatgaaagaa gcgatagcgc agcgtaaacc agtggttacc accgctgggc
    10741 cgcgtagtct gattttccgt ggggccatta ccggtgtaga taccagcaaa gaagggctgc
    10801 aattctatga agtggttcct gttgcattag tggttgcggg gacgcaaatg gctacaggcc
    10861 accgtaccat ggatactcgc ctctattttg aaggtgagct gattgatgca gcgactaata
    10921 aaccggttat caaagtcgtt cgtcagggcg aaggtaaaga cctgaataac gaaagtacgc
    10981 caatggcttt cgaaaatatt aaacaagtta ttgatgacat ggcgaccgat gccaccatgt
    11041 ttgacgttaa caaaaagtag ttccagacgc gccatcgtta gatggcgctt tttatccggt
    11101 gcgccgtaaa accccatcct tcagggcggg gatataaggc gcggttttcc acctgaccag
    11161 gtgtttgctg ttgttcaatg tattggcgga tgacggatat tggcgcacct ccgcagctac
    11221 tggcaaaata gccaggactc cacaaaacgc ctttgtagta atacctgact gcaatatctg
    11281 gtcgatctcg tcgcagtaac ctacccgata cgcctttgag gctgtttacc agactggata
    11341 tcgccagttt gggaggttag ttaattaaca
//
LOCUS       DAJTQY010000114        10963 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.114, whole genome shotgun sequence.
ACCESSION   DAJTQY010000114 DAJTQY010000000
VERSION     DAJTQY010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10963
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.114"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            <1..241
                     /locus_tag="OO349_003359"
     CDS             <1..241
                     /locus_tag="OO349_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5868316.1"
                     /translation="NGSVRYCQYTSMFGASYRYSGAGVGYQVASVVGGGFTPFIAAAL
                     ITYFAGNWHSIAIYLLAGCLISAMTALLMKDNQRS"
     gene            343..1797
                     /gene="amn"
                     /locus_tag="OO349_003360"
     CDS             343..1797
                     /gene="amn"
                     /locus_tag="OO349_003360"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HCR5868317.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGKYITIGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSADLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDIWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
     gene            2140..2856
                     /locus_tag="OO349_003361"
     CDS             2140..2856
                     /locus_tag="OO349_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR5868318.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPADLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
     gene            complement(3312..3387)
                     /locus_tag="OO349_003362"
     tRNA            complement(3312..3387)
                     /locus_tag="OO349_003362"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3352..3354),aa:Asn,seq:gtt)
     gene            complement(3488..5115)
                     /gene="yeeO"
                     /locus_tag="OO349_003363"
                     /pseudo
     CDS             complement(3488..5115)
                     /gene="yeeO"
                     /locus_tag="OO349_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
     gene            5120..5195
                     /locus_tag="OO349_003364"
     tRNA            5120..5195
                     /locus_tag="OO349_003364"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5153..5155,aa:Asn,seq:gtt)
     gene            complement(5233..6183)
                     /gene="cbl"
                     /locus_tag="OO349_003365"
     CDS             complement(5233..6183)
                     /gene="cbl"
                     /locus_tag="OO349_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HCR5868319.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEF
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGSPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFCWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRTDDAFTRKGLPA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLEFCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(6285..7202)
                     /gene="nac"
                     /locus_tag="OO349_003366"
     CDS             complement(6285..7202)
                     /gene="nac"
                     /locus_tag="OO349_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HCR5868320.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVAILEGELN
                     QQLLIRTKRGVTPTDAGKIIYTHARAILRQCEQAQLAVHNVGQSLSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQNVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            7530..7605
                     /locus_tag="OO349_003367"
     tRNA            7530..7605
                     /locus_tag="OO349_003367"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7563..7565,aa:Asn,seq:gtt)
     gene            complement(7661..8596)
                     /gene="ldtA"
                     /locus_tag="OO349_003368"
     CDS             complement(7661..8596)
                     /gene="ldtA"
                     /locus_tag="OO349_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR5868321.1"
                     /translation="MMRRVNILCSFALLFASQNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKPGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQHRSGMPVQISSGSRQMF"
     gene            complement(8658..9737)
                     /gene="cobT"
                     /locus_tag="OO349_003369"
     CDS             complement(8658..9737)
                     /gene="cobT"
                     /locus_tag="OO349_003369"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HCR5868322.1"
                     /translation="MQIIADLLNTIPAINSAAMSRAQRHVDGLFKPVGSLGKLEALAI
                     QLAGMPGVNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGIAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMDMRLGEGSGAALAMSIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(9749..10492)
                     /gene="cobS"
                     /locus_tag="OO349_003370"
     CDS             complement(9749..10492)
                     /gene="cobS"
                     /locus_tag="OO349_003370"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HCR5868323.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGAPLAALFSVLVLALMTGEFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLLGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(10489..>10963)
                     /gene="cobU"
                     /locus_tag="OO349_003371"
     CDS             complement(10489..>10963)
                     /gene="cobU"
                     /locus_tag="OO349_003371"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HCR5868324.1"
                     /translation="SQVLYIATSQILDDEMAARIEHHRQSRPEHWRTVERWQHLDELI
                     HADINPNEAVLLECVTTMVTNLLFDYGGDKDPDEWDYQAMEQAINAEIQSLIAACQRC
                     PAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQQLAAAANEVWLVVSGIGVKIK"
BASE COUNT         3044 a         2792 c         2372 g         2755 t
ORIGIN      
        1 aaatggtagt gtccgctatt gccagtacac ctcaatgttt ggtgccagtt atcgctacag
       61 tggtgctgga gtcggttatc aggttgccag tgtggttggc ggtggattta caccttttat
      121 tgccgctgca ctcatcactt actttgccgg gaactggcat agcatcgcca tttatttgct
      181 ggctggatgc ctgatttctg caatgaccgc tttattgatg aaagacaatc aacgctcgtg
      241 atagcctggc gaagatcatc cgatcttcgc cttatacttt tgtttcacat tcctgtgaca
      301 tactatcgga tgtgcggtaa ttgtatggaa caggagacac acatgaataa taagggctcc
      361 ggtctgaccc cagctcaggc actggataaa ctcgacgcgc tgtatgagca atctgtagtt
      421 gctttacgca acgccattgg caaatatatt acaattggcg aattacctga tgaaaacgcc
      481 cgtaaacaag gtctttttgt ctatccatca ctgaccgtaa cctgggacgg tagcacaaca
      541 aatcccccca aaacgcgcgc atttggtcgt tttactcacg caggcagcta caccaccacg
      601 attactcgcc ctactctctt tcgttcgtat ctgaatgagc aactaacgtt gctttatcag
      661 gattatggtg cgcatatctc tgtgcaaccc tcgcaacatg aaatccctta tccttatgtc
      721 atcgatggct ctgaattgac acttgatcgc tcaatgagcg ctgatttaac tcgctacttc
      781 ccgacaacag aactggcgca aatcggcgat gaaactgcag acggcattta tcatccaacg
      841 gaattctccc cgctatcgca ttttgatgcg cgccgcgtcg atttttccct cgcacggttg
      901 cgccattata ccggtacgcc agtagaacat tttcaaccgt tcgtcttgtt taccaactac
      961 acacgttatg tggatgaatt cgttcgttgg ggatgcagcc agatcctcga tcctgatagt
     1021 ccctacattg ccctttcttg tgctggcggg aactggatca ctgccgaaac cgaagcgcca
     1081 gaagaagcca tttccgacct tgcatggaaa aaacaccaga tgccagcatg gcatttaatt
     1141 accgctgatg gtcagggtat tactctggtg aatattggcg tgggaccgtc caatgctaaa
     1201 accatctgcg atcatctggc agtgctacgc ccggatatct ggttgatgat tggtcactgt
     1261 ggcggattac gtgaaagcca ggccattgga gattatgtcc ttgcacacgc ttatttacgc
     1321 gatgaccacg ttcttgatgc ggttctgccg cccgatatac ctattccgag cattgctgaa
     1381 gtgcaacgtg cgctttatga cgctaccaag ttagtcagcg gcaggcctgg tgaggaagtc
     1441 aaacagcggc tgcgtacagg tactgtggta accacagatg acaggaactg ggagttacgt
     1501 tactcagctt ctgcacttcg ttttaactta agccgggccg tagcaattga tatggaaagt
     1561 gcaaccattg ccgcgcaagg atatcgtttc cgcgtgccat acgggacact actgtgtgtt
     1621 tcagataaac cgttacatgg tgagattaag cttcccggcc aggctaaccg tttttatgaa
     1681 ggcgctattt ccgaacacct gcaaattggc attcgggcga tcgatttgct gcgcgcagaa
     1741 ggcgaccgac tgcattcacg taaattacga acctttaatg agccgccgtt ccgataataa
     1801 gttatgaacc ttcttatcag aaatagatgt gaaggattaa gtaagacatc tggcaaatag
     1861 cctgcaataa acgcgggctg cttgcttcct tgggcagatg cgagttttat tatcgtctta
     1921 atgatttcca catattaaaa gcaagtatgc tttcaaaaca caattataaa aaatcccgcc
     1981 aacaacatta gtttttataa aattaaatat aagattatgg ctttagaata tttttatttc
     2041 taatagacga gatttttcct gttatgatat aatatgccga attaacacat gttaacgatt
     2101 taccagtaat gtaaataaat tttcgaggag atcattccag tgggacgtaa atgggccaat
     2161 attgttgcta aaaaaacggc taaagacggt gcaacgtcta aaatttatgc aaaattcggt
     2221 gtagaaatct atgctgctgc caaacaaggt gaacccgatc cagaattaaa cacatcttta
     2281 aaattcgtta ttgaacgtgc aaagcaggca caagttccaa agcacgttat tgataaagca
     2341 attgataaag ccaaaggcgg cggagatgaa acgttcgtac agggacgtta tgaaggcttt
     2401 ggtcctaatg gctcaatgat tattgctgag acattgactt caaatgttaa ccgtacgatt
     2461 gctaacgttc gcacaatttt caataaaaaa ggcggcaata tcggagcggc aggttctgtc
     2521 agctatatgt ttgacaatac gggtgtgatt gtatttaaag gaacagaccc tgaccatatt
     2581 tttgaaattt tgcttgaagc tgaagttgat gttcgtgatg taactgaaga agaaggtaat
     2641 attgttattt atactgaacc tgctgacctt cataaaggaa tcgcggcgct aaaagcagct
     2701 ggaatcactg agttctcaac aacagaatta gaaatgattg ctcaatctga agttgagctt
     2761 tccccagaag atttagaaat ctttgaaggg cttgttgatg cccttgaaga tgacgacgat
     2821 gtacaaaaag tttatcataa cgtcgcaaat ctctaattat attttaaaga aatctgtctt
     2881 tatggcagat ttctttaatc tcatataatt cttataaaaa atataatatt taactcgtca
     2941 tattgcttat aaaaaatccc cgtccccaga gaaataatat ttattaaaat ggcaggtctt
     3001 ctttttctga ttaccgaaga caaagtggca attacgtata gtttcccgat aaagacgcga
     3061 cagctacgtc ccgcataagg catttttctc tttatctttg tacggtactt catggaacag
     3121 aatttttgac cttgtgaatc gtgatatctg ttggggaggg gcaatttgct cactgaagcg
     3181 tgagactcga ttaagcgcac gaaacacaga aatcaaaaaa cccggccact tttttacaaa
     3241 gtggccgggt aaaaataatt tttatttttt aactgtcctg agtctcatag agatgtctca
     3301 aaactaaaat ttggctcctc tgactggact cgaaccagtg acatacggat taacagtccg
     3361 ccgttctacc gactgaacta cagaggaatc gtgagaacga ggcgaatatt agcgatgccc
     3421 acccacaatg tcaaagcctg ttttttaaat ttgaaatcgt ttgctgaaat aatctgcatt
     3481 ttgtcgttta ttccgacaca actggctttt tttcacactt ttgcggctcg gatcgagggt
     3541 atttccacag ccaacgtcca gtaaccattc gccagtaaaa cagcacggcc cgcacagccc
     3601 agtcggcaaa cattcccatc cagacaccaa ccacacccca gccaagcatg attccctgca
     3661 cataaccgac tacaacccgg caaccccaca tgctcaacat agaaacccac atggcgtaac
     3721 gggcatcacg agcaccctta aatccagcgg gtagcaccca tgaggcggac caaataggca
     3781 taaataaagc atttagctaa atcagaatca caacgacgtg tttaacctgt ggatcctggg
     3841 tgtaaaacga tgccataacc ccggcaaagg gagccgttag ccaggcgatg gccgttaatc
     3901 caagagtgga cagccagaac acatgccgca actgaatctc tgcttgcgct atctgcccta
     3961 cccccaacct tcggcctgta atgatcgtag aagcagaacc gagcgcactt ccgggtaagt
     4021 tgataagagc cgcaattgag aacgcgataa aatttccggc aataacgctg gtccccatcc
     4081 cggcaacgaa catttgggtt aataaccgac cactggtaaa taacactgat tcgacactcg
     4141 ctggaatacc aatccccatg acttcccaga taatgctaaa attcagcggt ttaaaatagc
     4201 tctttaacga aatccttagc gcaggattaa aaccaatcgc cagcacccac aaaattgcaa
     4261 ctgcgccaat ataacgagaa atggttaaac ccaacccagc cccgaccagg agaaaaggcc
     4321 gtaaatcaat atgccgctaa taataatatt aagaatattc aggctaccgt taatcagtag
     4381 cggtattttc gtattccctg caccacgaag tgccccgcta ccaataagag tgatggcagc
     4441 ggctggataa ctgagtacgg tcagctccag ataagtcaac gccagtgctt taacttctgt
     4501 cgtggcatca cccgcgacga aatcaataat ttgttcgcca aaatgatgaa ttagcgttgc
     4561 caacagtacg gcaaacaacg tcatgatcac caatgactgc cgcgtcgcca ccctcgctcg
     4621 tcgtcgatcc cgcttaccga gactaaatgc tacaacgaca gtagtaccaa gatcgatagc
     4681 tgcaaaaaaa gccataatga ccatattgaa gctgtccgcc aaccccacgc cggccatcgc
     4741 atcttttccc agccaactga ccagaaaagt gctcaggccc cccatcaaca ggacacaggc
     4801 gttctccatg aagataggaa cagcaagcgg ggttatctcg cgctagaaca acactttgta
     4861 gctcttgcgt ttagagtgcc agcgagtgcc gtgaacaacc tggcgtaaag cagaggagat
     4921 attcaaagcc gaccttaatt gcagaaagtg aaaccacatt tcaaatgatg agggagaatc
     4981 agcaaagctg caaagatttt cgccaacaaa ttgtctgcaa atgcaacaaa ctgttgatag
     5041 aaacggcaaa cagttgggaa atttaaaaat cgggtttgac aaaatatttt tcgccgttaa
     5101 gatgtgcctc aacagcgatt cctctgtagt tcagtcggta gaacggcgga ctgttaatcc
     5161 gtatgtcact ggttcgagtc cagtcagagg agccaaattt ctgaaaagcc cgcttttata
     5221 gcgggctttt tgctatatct gataatcaat ttcctcttca ctgttttcca tcacctggcg
     5281 cttgatatcc tcaactgaca gtcctgcatt acaaaattcc agaaagcgcc aaacatagtt
     5341 acgctgaagt tgtcctcgct tcagtcccaa ccagacagta ttagcatcga aaagatgccg
     5401 cgtatccagg cggattaaat tctcttcctc ttgttcgccg ctggattgtt cggcaactaa
     5461 tccgatccca agcccaagag caacataggt tttaatgaca tcagaatcct gtgcacttaa
     5521 tacaatatct gccggcaaac ctttgcgggt aaatgcgtca tcagtgcgtg agcgccccgt
     5581 aattccctgt cggtaagtaa ttaacggcca cttcgctatt gattccagcg tcaatggtgt
     5641 aatttgcgtt aagggatgat cgagtggaac aagcaaacta tggtgccaac aaaaccacgg
     5701 gaaggcgacg agctgcgggt cattactcaa acgctcgctg gcgataccaa tatcagcttc
     5761 gccattttgc aacaacgtcg caatttcctg tggcgacccc tggattagct cgagccgaac
     5821 ctccgggaaa agttcgcgaa aagctttaat gacctctggc aagctataac gtgcctgagt
     5881 atgcgtcgtt gcaatagtga gaacaccaga cgtatcgttg gtaaacaggt ctgcaagccg
     5941 acgaacatta ctggcttcat tcagaatacg ttctgcaatg accagtaatg ctttgcccgg
     6001 ttcagtcatc cccagcagtc gcttacctcg tcgaacaaat atttcgatgc caaattcatc
     6061 ctccagttcc cgaatatgac ggctaacgcc tgactgtgag gtaaaaagca tattcgcaac
     6121 ctctgtcagg ttgtaatcct gacgtgcagc ctcgcggatt atctttagtt gttggaaatt
     6181 cacggtaaac tccgggcagt tcagatatcc cgttattgtt aaagtctaat gcccggcata
     6241 acaaataata aaaacccgca tcttattcca tcccgatata acgcttagct caccaattgc
     6301 cactgccttt tttccatcac tggagaacta atcactgaca ttaacaactc tttcactgcc
     6361 tgtgcctgtg gtgacaagtt tgctctggcg ggtaaattta aggacaaaga gagactcatt
     6421 gaaggagtgg taatgcgtga catccaccca tttactgcgc cacataacga acgcgcagcc
     6481 gattcaggta atactgcaac gcccatgccg ctggcaatcg ctgcggtaag cgtggcaata
     6541 gactcaattt caccaatgac ttttgccgtg agccgccgta gggaaaaagc ctcatcaaca
     6601 cgaagtctaa cagcgctgta atcactgggg agaaaaaggt tcatttgcgc aatagcatta
     6661 acatcaacgt tttgccccgg gcaatcttga gttcctacca ggaaaagatc ttctttcagc
     6721 aaagcctgac tggatacccc agccacaggg gaatgctcat aaatcaccgc catatcgagt
     6781 tggtgattta tcagtttttc gttaagcact gcaccactat tttcatgaag atagataacg
     6841 acctccggga attcagcgcg aaccgcctgt aataagggca tggtgatgga tgacgcagcg
     6901 gttcctggcg caaagccaat cgagacttgc cccgataatg actgaccaac gttatgcacc
     6961 gccagttggg cctgctcaca ctgacgtaaa atggcccgtg catgggtata gataattttt
     7021 ccggcgtctg ttggcgtaac gccccgtttt gtgcgaatca aaagctgttg atttaactca
     7081 ccttccagta tggcaacctg ctggctgagc gctggttgtg cgatatgcaa tacttcagca
     7141 gcctgggtca ggctaccaat atctacaatt tttacgaagt atttcaggcg tctgaagttc
     7201 atgttgcctc cggtttttaa gaatcggccc aagtgccgcc attacttaca accagtatgc
     7261 aagatgcctg ccagttctat tttggcgttg atgcgcaagc taaccaactg tcaaataaga
     7321 gattatgata gattcgtcat tggccctttt tatcagcagt cgcattcacc tgccctataa
     7381 aaggagggta tgcaccacga tggttcatta cccaataaga ttgaaagctc accactttgt
     7441 tgaaattggc agcaaacaaa caaaaaaaat gcatttcacc ctttgacatc accatgcact
     7501 gccattaata tgcgccccgt tcacacgatt cctctgtagt tcagtcggta gaacggcgga
     7561 ctgttaatcc gtatgtcact ggttcgagtc cagtcagagg agccaaattc aaaaaagcct
     7621 gctttcgagc aggcttttta ctttctaatt accaacgctc ttaaaacatc tgtcttgaac
     7681 cagaactaat ttgcacaggc attcccgatc gatgttgcaa cgcagcattt gcgcgattta
     7741 catcaacttc ttgcccgttg ataaacgccc gcaaagatgg ggttaccggc aatggcactt
     7801 ttcggtcaga ctcatattct gcacgattgc gcgacagcgg ctcatgaact tccagccact
     7861 ttgaaccatc cggttccgtt gtgtatttca ctggctgatc aataatttgt acacgagtgc
     7921 ctacaggaac attatcaaac agatatttga tatcgtcatt acgcagacga atacagccct
     7981 gacttacacg gagcccaata ccaaaattgg cattggtacc gtggatggca tacaacctgc
     8041 caatataaat cgcgtacagc cccatggggt tatcgggccc cgcaggaaca aatgcgggca
     8101 aactctcccc tcgtttcgca tattcgcgcc gggtgttcgg cgttggcgtc caggttggtg
     8161 cttcttgttt acgttcaacg gtagtcaccc agttacgcgg ggtttctcgc ccagcctggc
     8221 cgataccaat aggaaagact tccactgtat tactgtctgg cggatagtaa taaagacgca
     8281 tttcagcgac gttaaccaca atccctttac gaacagtggc gggcaaaatc agttgctgcg
     8341 gaatggtgag ttgtgagcca ggcttcggca aaaaaacatc agcgcccgga ttcgcttcca
     8401 gcatattact taacccttgc ccgtattgtg cggcaaaagt ctccagcggc tgagtattgt
     8461 gatcaggaac agttacagta agcgactgcc ccactaaacg gctaccctct ggaggtaatg
     8521 gataagttac cgccaggcta ttttggctgg caaaaagcag agcaaatgag caaagaatat
     8581 ttacacgacg catcatgtcc ctttcctaag tcgcgaatac tatccgataa gtatagcttt
     8641 tatcagactt ttcgttttta gctgttcaaa tcagaagtcg tattccccgg tagaacaata
     8701 ttactggcag caagttcgcc catgttgttg tatatcgcac aagcagcttc gatgatggac
     8761 atcgccagag ctgcaccact cccctcacct aaacgcatat ccatattgag ataaggctcc
     8821 agccccaaat gcgagagcgc tatacgcgcg cctttttctg ccgacaagtg agaaggaatg
     8881 agatacggtt tgattgcagg agacatctgg caggctgcga gcgcagcagc ataagaaaga
     8941 aatccatcca gcaacacggg taaaccgcag gaagcagcac ctaacatcac tccagctatt
     9001 ccgaccaaat caaatccacc cacttttgcc aggacatcaa caccatcctg aggatttggt
     9061 tgattcaacg taatcgcccg acgcacaaca tcaattttat tagccagttt atctgtcggc
     9121 aggtttgcgc caatcccaac cacttcttca ggatcccggc cagtgattgt gctgactatt
     9181 gccgctgccg gtgtcgtgtt tgccatcccc agttcaccta caccaaacag cgtgacaccg
     9241 tttttcgcca gctcccgcgt ataacatatg acgtccaaaa gcaacttttc agcctgacgg
     9301 cgactcattg ccggagctga agcaatattg ccgctacctc gtgcgacacg catgttgata
     9361 agcccgggga taggctcagc agtatcaata ccaacatcaa ttacgtggac gttagcgccc
     9421 gcttgtgctg ccagcacaca cacgccggtt gttccacggg tcatattttc agcctgtatg
     9481 gctgtcactt cttttggaga aatagcgacc ccttcctccc agacgccgtg atcggcacac
     9541 ataaccagta ccgctttttt gcccacatgc ggtatgccat tcacccccgg cattcctgcc
     9601 agttgtatgg caagcgcctc cagctttcct aggctaccaa caggtttgaa taacccgtca
     9661 acatgccgtt gtgcacgcga catagcggca gaattgatgg ctgggatcgt attcagtaaa
     9721 tcggcaatta tttgcatgtc acgtttcttt atagcagtgc cagcaggaag accagttcac
     9781 caagctctat ggctgctccc agcgtatcgc ccgtttgccc gcccagcgta cgtttgagaa
     9841 gctgaccgag gatgaaaatc gccaccatcg tcaccaccat agcagccaca ccatgcatac
     9901 ccagcaataa tacggcagca aaaatagcgg ctaagccgag tgtgacgcag gtttgtcgcc
     9961 catcaatttt gccaatgaac acattgccaa gcccttcttc ccgcgcataa cgatgacgat
    10021 acatcaataa tgcggcagtc ccgcggctga ccgcacatgc tgccgccagc gatgcaagga
    10081 tcggttcgcc acgcagagcc aactcgctta acaccagaat ctttgccagt accacaaaaa
    10141 tcaatgccag accgccgtgg gtgcctaaac ggctatcacg catgatttcc aacatgcgat
    10201 cgcgactacg tgcagaaaat acgccatcac aggtatcagc gaggccgtcc agatggaatt
    10261 ccccggtcat cagcgccagc acaagtacgc taaacagtgc cgccagtggt gcgccacacc
    10321 atgcctgcag caccatgaag accagcccgc taatcgcgcc aagcaataat ccaatcaaag
    10381 gaaaagtaat aataccgcga gaataatgct cgaaatccag tccctgggac cagcgacgcg
    10441 gtacaggcag acgcgtaata aatgagagca tcgcccaaaa taatttgctc atttgatttt
    10501 tactccaata cccgaaacca ccagccatac ttcatttgcc gcagcggcca actgctgatt
    10561 tacccgcccg gcaatatcac gaaaatgtcg tgccagacga ctctccggca caatccccat
    10621 tcccacttcg ttagtcacta atacaacctt tgcggggcaa cgttggcagg cagcaatcaa
    10681 cgactgaatc tcagcattaa tcgcctgttc catcgcctga taatcccatt catcagggtc
    10741 tttatcgccg ccataatcaa acaacagatt agtcaccatt gtggtaacgc attccagcaa
    10801 cacagcctca ttcgggttaa tgtctgcatg aattaattca tcaagatgtt gccagcgctc
    10861 cactgtgcgc cagtgctccg ggcggctttg ccgatgatgt tctatccgtg cagccatctc
    10921 atcatcaagg atttgcgagg tagcgatata cagaacctgt gaa
//
LOCUS       DAJTQY010000115        10924 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.115, whole genome shotgun sequence.
ACCESSION   DAJTQY010000115 DAJTQY010000000
VERSION     DAJTQY010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10924
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.115"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            152..502
                     /locus_tag="OO349_003372"
     CDS             152..502
                     /locus_tag="OO349_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HCR5868325.1"
                     /translation="MLILDSRPVHASRPHSEAIRDAQRKKPKVPVHAVLTATNPLIRF
                     IGSDDMTQNRELFQVWLQKLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPE
                     IGAVPAIPQQSSLF"
     gene            555..950
                     /locus_tag="OO349_003373"
     CDS             555..950
                     /locus_tag="OO349_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HCR5868326.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            991..1734
                     /gene="cmoA"
                     /locus_tag="OO349_003374"
     CDS             991..1734
                     /gene="cmoA"
                     /locus_tag="OO349_003374"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HCR5868327.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            1731..2702
                     /gene="cmoB"
                     /locus_tag="OO349_003375"
     CDS             1731..2702
                     /gene="cmoB"
                     /locus_tag="OO349_003375"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HCR5868328.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     TVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(2867..5296)
                     /gene="torZ"
                     /locus_tag="OO349_003376"
     CDS             complement(2867..5296)
                     /gene="torZ"
                     /locus_tag="OO349_003376"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HCR5868329.1"
                     /translation="MALTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMSVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHERIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQASWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAVWTAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQRANACAGNSALVYIEKYTGNAPK
                     LTAFDKPAVQA"
     gene            complement(5321..6421)
                     /locus_tag="OO349_003377"
     CDS             complement(5321..6421)
                     /locus_tag="OO349_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HCR5868330.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHASIAADHYTVNAWPSIA
                     KGMGARTSMSENEPDILTRYFQYNAKDITEKQ"
     gene            complement(6809..7555)
                     /gene="cutC"
                     /locus_tag="OO349_003378"
     CDS             complement(6809..7555)
                     /gene="cutC"
                     /locus_tag="OO349_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HCR5868331.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(7569..8135)
                     /locus_tag="OO349_003379"
     CDS             complement(7569..8135)
                     /locus_tag="OO349_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR5868332.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSEKMINGRPICLFKLHEPVQVAHWQFSNVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            8351..10084
                     /gene="argS"
                     /locus_tag="OO349_003380"
     CDS             8351..10084
                     /gene="argS"
                     /locus_tag="OO349_003380"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HCR5868333.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQIRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDTAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            10261..10749
                     /locus_tag="OO349_003381"
     CDS             10261..10749
                     /locus_tag="OO349_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HCR5868334.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDTSAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGAKKLDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
BASE COUNT         2669 a         2788 c         2605 g         2862 t
ORIGIN      
        1 atgggctttt aatcctatcg tttattgctt gcatagtaga atctaaccat ccatcactca
       61 tagctaaccc tcggaaatct tacatttctg tctatcataa ccactagcat cacctttagg
      121 aaccatctga aacgggaggt gtatttagac cttgctaatc ttagacagcc gcccggttca
      181 tgcatcacgt ccacacagtg aagctattcg cgacgctcaa cgaaaaaaac ctaaagttcc
      241 ggtacatgct gtactgacgg cgacaaatcc gctgatccgt tttatcggta gtgatgatat
      301 gacgcaaaac cgggaattat ttcaggtctg gttacaaaaa ttagcgcagt ggcatcagac
      361 cactacgcct tatctttttt tacatacgcc agatattgcc caggccccgg aactggtaca
      421 taccctgtgg gaagacttac gtaaaacgct tccagagatc ggagcagttc cggctattcc
      481 acagcaatct tctcttttct gaatttgcca cctatcatag acaggtgcca tcggccattt
      541 taaagggagt ttgtatggta agcgcgctat atgccgtttt aagtgcgttg ttattaatga
      601 agttctcttt tgatgtcgtt cgcctgcgaa tgcagtaccg cgttgcctat ggtgacggtg
      661 gttttagcga actgcaaagc gctattcgca ttcatggtaa cgcggtggaa tatattccca
      721 tcgcgattgt gctgatgctg tttatggaaa tgaatggcgc agaaacctgg atggtgcata
      781 tttgcggcat cgttttgctt gctggtcgtc tgatgcatta ttacggtttt catcaccgtc
      841 tgtttcgctg gcgacgttcc ggcatgagcg ccacctggtg tgcgctgttg ctgatggtgc
      901 tggcgaatct ttggtatatg ccctgggagt tggttttctc cctgcgttag cgcacaatac
      961 gccactttct ttttcccgga tttttacgtt atgtctcacc gcgacacgct attttctgcc
     1021 cctatcgcca gactgggcga ctggaccttt gatgaacggg tcgctgaagt cttcccggat
     1081 atgatccagc gttccgttcc cggctattcc aatattattt ccatgattgg tatgttagcc
     1141 gagcgcttcg ttcaacctgg tacgcaggtt tacgatctgg gttgttctct gggcgcggcg
     1201 acgctctcgg tgcgtcgcaa cattcatcat gataattgca aaattattgc catcgacaac
     1261 tccccggcga tgattgaacg ctgccgtcgt catattgacg cctataaagc ccctacgcca
     1321 gtagacgtca ttgaaggtga tattcgcgat atcgccattg aaaacgcatc gatggtggtg
     1381 ctgaatttta ccctgcaatt cctggaacct tccgagcgcc aggcattact ggataaaatt
     1441 tatcaaggac tgaacccggg cggcgcgctg gtgctttcgg aaaaattcag tttcgaagat
     1501 gccaaagttg gtgaactgtt gttcaacatg caccacgact ttaaacgtgc caacggttac
     1561 agcgaactgg agatcagcca gaagcgcagc atgctggaaa acgtgatgct gaccgattcc
     1621 gtggaaaccc ataaagcacg cctgcataaa gccggttttg agcatagcga gctgtggttc
     1681 cagtgcttta actttggttc actggtggca ttaaaagcag aggacgctgc atgatcgact
     1741 ttggtaactt ttattctctg attgccaaaa atcatctttc acactggctc gaaacgctgc
     1801 ccgcgcagat tgctaactgg cagcgcgagc agcagcacgg gctgtttaag cagtggtcca
     1861 acacggtgga atttctgcct gaaattaaac cgtatcgtct ggatttattg catagcgtaa
     1921 ccgccgaaag cgaagagcca ctgagcaccg ggcaaattaa acgcattgaa acgcttatgc
     1981 ggaacctgat gccatggcgc aaagggccgt tctcactgta tggcgtcaac atcgataccg
     2041 aatggcgttc cgactggaaa tgggatcgtg ttctgcccca tctttctgat ttaaccggac
     2101 gcaccattct ggatgtcggc tgcggcagcg gttatcacat gtggcgcatg attggcgcag
     2161 gcgcgcacct cgcggtgggg atcgacccta cgcaactgtt tctgtgccag tttgaagcgg
     2221 tgcgtaaact actgggtaac gatcagcgcg cacatttgtt accgttaggt attgaacagc
     2281 ttccggcact gaaagccttt gataccgtct tttcgatggg cgtgctttac catcgccgtt
     2341 cgccgctgga gcatctctgg cagttgaaag atcaactggt gaatgagggt gaactggtgc
     2401 tggaaacgct ggttattgat ggcgatgaga acacggtgct ggtaccaggc gatcgttacg
     2461 cgcaaatgcg taatgtctat ttcatccctt ccgcgctggc gctgaaaaac tggctgaaga
     2521 agtgtggttt tgtagatatc cgcattgtgg atgtgtgcgt taccactact gaagagcagc
     2581 gacgcactga atggatggtt accgaatctc tgtccgattt cctcgaccca catgatccga
     2641 gtaagacagt tgaaggttat cctgcgccta aacgcgcggt gctgattgcg cgcaagccgt
     2701 aaaggtctgg tattactgcc ggatgcggcg tgaacgcctt atccggccta caaacccttg
     2761 ctaattcaat ctattgcagg gactatgtag gcctgataag catagcgcat caggcagttt
     2821 tacgtttgca taacctcagc gcccgtttcc gggcgctatt cacttcttac gcctgaacag
     2881 caggcttatc aaatgccgtt aacttcggtg cgttacccgt atatttttcg atatacacca
     2941 gcgcagagtt accggcacag gcatttgccc gctgcgagct ggggatatcc gccgttaaca
     3001 cgttcgcact gccgttttta cacaagccgt tttccagatc tggccatgca ccttcatgca
     3061 cgcataccac gccttttttg atcccgtcag tcaccaccgc gcctgtcaaa atctgaccac
     3121 gtttgttcca gacacgcacc agatcgccat tcgcaatacc aaatcgagca gcatcttcgg
     3181 tgtgaatagt gatcggttca cgatcggcaa ccgcatattt tttacgcagt tccgcatagt
     3241 taagctgact gtgtaaacgg tgtgccggat gtgcggtcag aagctgcaac tgcttctcgt
     3301 cggcggtacc cttccactca tcaggcgcaa gccaggttgg gtgtgccggg caatccttat
     3361 agccaaattt ttccagcgtt ttggagtaaa tctcaatttt gccgcttggc gtacccagcg
     3421 catttttcac cggatcggcg cggaaatcac cataacgaac gtactgttcg ttcttctcgc
     3481 tgcggcgcat ttcgatcagt ttattttgct gccagaaggc attaaacatt ggcatagtga
     3541 cgcgttgcgc acgggcccct ttctgagcgg catcatagaa aaatttcagc cacgccattt
     3601 catctttacc ttcggtatag atctcttttc cgccaggttt gagtaattcc gcaagatcgg
     3661 caaacacgtc aaaatcgtta cgcgcttcaa attgcggagc aacagcctgc ttcatcggca
     3721 caatatgctg gttgctgtaa tcaccggtca tcgtcaagtc attgcgctca aacgatgtgg
     3781 tgatcggtaa tacgatatct gcgtgtttgg ctgctgcggt ccagtagcat tcagaaacga
     3841 cgatcatctc cggtttctgc caggctttga tcagacgatt agtatcctgg tgatgggtaa
     3901 agttaccgcc acccgcccac cagatcattt tgatattcgg atacgtttgt tctttaccgt
     3961 tatgctgata tttgccgcca ggattttcca gcgcatcgac gatacgggca acaggaatcg
     4021 ccgtcattcc gccatcatca gcggcctcgc tggcctgtcc ggcaatcgcc gcagacattt
     4081 ccggcagcac gccgccaaca cgcgtcgggt tgccaccatt ggagtaatga taagagaaac
     4141 caaaaccgcc gcccggtgta ccaatctgcc ctaacatcgc cgccagcgtc accagcatcc
     4201 agtgtttctg ttcaccatat tgctggcgct gaattcccca gcctgccatc agcattgtgc
     4261 gattcgccgc catcagttca gcgagtttga ctatctgcgc ttccggtacg ccagtaattt
     4321 ccgccgtcca gaccgcgctc ttcggcgtat tatcgctctt acctgtcaga tactcttcaa
     4381 actgcggata cccggtagtg tatttttcaa ggaacacttt atcgtgtttg ccttgtgtca
     4441 tcagggtatg cgcaatccct aacatcagtg ccacgtcggt gcccatattc ggcgcgatcc
     4501 aggtggcatt atcgccaaag aattcgatgg tttcggagcg gataggatca atggcaatca
     4561 ctggtttgcc agattttttc agctgatgga agtattccag cccttgctca tcggtactgc
     4621 tccaggcaat ttttaaggta ttcagcgggt tcattcccca cagcaccaca acctggctgt
     4681 tttccagaat cagcggccag gaggcctgct gttcatacac ctctacagaa ccgaccacat
     4741 gcggcatgat cacctgtgct gccccggttg aataatcgcc gctatgcccg gaataaccgc
     4801 ccgccaggtt catataacgt tgcagtaagg tttgcgcttt atgcaacacg ccagaagagc
     4861 gccagccgta agatccggca aaaatggccg atggtccgtt agctttacga atacgctcat
     4921 gttgctcatg aatcagtttt aatgcctgtt cccagctcac ctgtacatag gtatcttcgc
     4981 cacgaccttt cgccggttgc agtggattgt cgagatagct ttttctcacc atcggatgct
     5041 gaatacgcgc cgtggtgtgt acctgatccg ccgccgtaga ctgtaaggag ttcggtatgg
     5101 ttttcgccag cgcgcctgtc gaagaaacaa tcttgccgtc cttcacttct acgctcatcg
     5161 ctccccaacg tcccgcggtg aggattttac cgcccttctc ttctgcccat gcggggagcg
     5221 gtgctgccga tgtcaccacc agcgctccag cggcaatacc gctgtgttta ataaattcac
     5281 gtcttgttaa tgccataact tcctccctga tcaacgagga tcactgtttc tcggtaatat
     5341 ctttggcgtt gtactggaaa taccgcgtta aaatgtccgg ttcgttttcg ctcatgctgg
     5401 ttcgtgcccc cattcctttg gcaatggacg gccacgcatt gacggtgtaa tggtcggcgg
     5461 caatagaggc atgacaacca gcgcaatagg tatcgtcaag tttttcagcg tattgccata
     5521 gcggtttacg gtccgctaat gcgggatcgg taagcgcacc ctgtaaagac gcctgacgcc
     5581 attgattgcc gtattcgtca gcctgccatt ccccgtttac cgtgagcgcc ttgatacctt
     5641 cttcacttaa tgtggctagc gccagccgtt gacctgccgc caggtagagc gtgttttcac
     5701 tgccctgcat ttgataaccc tgcaacagaa cgatcggctg tttgccactg gcatcaacga
     5761 cggtgagatc ggtaccagga ttcacggtag ccagctcgcc tatgcgagaa gttttgaaag
     5821 gataaatatg tgcgccatta gtcactgaag tagcggcctg actttccagc tcatgcgccg
     5881 cgttgtcatc catttttatt tctggcggaa aatgggcaat gcctttatga caatcgatac
     5941 aggtttcgcc gcccttttgt gctttgttat gcattttctg cgcagattca ctttgcgagg
     6001 caatatccat ggcatcaaaa gaatggcaac tacggcacgt tgcagagtca gttgctttta
     6061 attctttcca tactgtttcg gccatttcct ggcgatgagt ttcgaactta tcgtcactgt
     6121 ctattttgcc gctaacaaat tcatgataaa tatctttaga tgcttttaat ttagcaaata
     6181 aataatccat ccctgacttt ggaatatggc aatcggcaca ttccgcacgt atccctttct
     6241 ggttcgaaaa gtggacagtt ccctgatatt cctcaaaagg tttactcatc gagtggcagg
     6301 aaaggcaaaa tgctgtatcc gacgttttat gtaaggcttt ttgcgccagc aataacccgc
     6361 cgccaccaac cacaaccgct atcagcaaaa acaataaccc aatgcgtttt ttccctcgta
     6421 tagattcctc taaataaatt ccagaacacc gaaatcattg tataattaaa tacacaacga
     6481 attaaccgct tgcataactc ggcacaacac tgcctgaaat aatcgataaa gaatatgatt
     6541 tattacaatg taatcattaa ttgctaagga ataacccagt tgctatttag aatatatatt
     6601 tatcagtttt agcaatttaa atcccataat caatgtgaat ataaaaaata tttttagcac
     6661 aacgctatgc gcactgtcac ccgctcaaca gatcacggtt atcgttcgtt ttttatactg
     6721 ctcagggata aaaaaaggcc cctgttgaaa ttgcaggggc ctggtacgag caagcatcat
     6781 attgggcgac atgatgcaac ggtaaaaatc atttggcctg atggcgttca atgattcctt
     6841 tcatttcagc aaccgccgcc ccgtctacga tataacgcga atactcgtcc gcgtgttcat
     6901 ctgatgacat ggacaatcct tgattacgat aacgcatcgg tgaggcttgc cacgctcccg
     6961 cggagctatg gacttccagc actccggcat cgaggaagtg gtgcaggttt tctgcacgga
     7021 ctcctgctcc agccataatg attggagcat cacgatgggc aataagttcc ataatttttg
     7081 ataaaccttg cagtgcgtct gatttttgcc ctgatgtcag tacccgggca atgcccaatt
     7141 ccgcaagatt attgagtgta tataaagggt tagcgcacat atcgaaggcg cgatgaaaag
     7201 tcactgccag cggaccggca gcagccatta ttttttccat tcgtggcata tcgacattcc
     7261 cgtcaacatc gagaacgccc gtcaccagtc caggaaaacc taattcgcgg accgtgcgca
     7321 catcctcaag aatggcggca aactcaccgt cgctgtagca aaaatcacca ccgcgtgggc
     7381 gaattatcgg atgcacaggg atcgtcaccc gctggcgcac ggatttcagt acccccaacg
     7441 acggcgttaa gcccccctct tttggggctg cgcataattc aactctgtct gcgccgtttt
     7501 gctgcgccgt tagtgcacat tccatgctgt aacagcaaat ttccagtaat gccatttttg
     7561 ctccttaatt acgctgactg ctcgctggca acgatctctt caatggacca cggatgaaac
     7621 ttaatagttg ttttgccatc ggtgaccgcc agcgtcggat tcggtaatcg ctcatgctca
     7681 ccttttggtg aactggtttt aactgagatc cccggtaggc tgagtccttc atcagaaagc
     7741 aatgccagcg cacgggcatt cagcgtttct ggatcacccg gcagaacgat ttcaatatgt
     7801 tcccaacctt cgtgtgggta acgtttttcc ccgggccacg gtagctccac attggaaaac
     7861 tgccagtgcg caacctgtac cggttcatgc aatttaaaca gacaaatcgg tctgccattg
     7921 atcatttttt ctgacaaaag ctcgccacac tgttcaaaac cgcgacgcca gcgctcagcg
     7981 gtgacgtttt gatggcaacg caaagaaatg tgatcggcag tcagcggagt gatatccaac
     8041 cccagacggc gggaaagttc atctaatgcg tgggtaaatc gcggtaaatc cgatgcaata
     8101 tcctgcagct cgtcgataga ttgccagttc gccataatca ctcttcgtct ttcagtaaaa
     8161 gcgttaattt accctgttgc cctgtgccaa ccaaccgctg atttcacgcc gcttctgatg
     8221 caatagtgaa aacggcaata cgccacgcgc acgttgctga cgaaaacagc catttgcagt
     8281 atactcccgc cctaatttct ttaactggtg cgggcaattt ttgctcgctt catcaatgta
     8341 aggtattccg gtgaatattc aggctcttct ctcagaaaaa gtccgtcagg ccatgattgc
     8401 ggcaggcgcg cctgcggatt gcgaaccgca gattcgtcag tcagcaaaag ttcagttcgg
     8461 cgactatcag gctaacggca tgatggcagt tgctaaaaaa ctgggtatgg caccgcgaca
     8521 attagcagag caggtgctga ctcatctgga tcttaacggt atcgccagca aagttgagat
     8581 cgccggtcca ggctttatca acattttcct tgatccggca ttcctggctg aacatgttca
     8641 gcaggcgctg gcgtccgatc gtctcggtgt tgctacgcca gaaaaacaga ccattgtggt
     8701 tgactactct gcgccaaacg tggcgaaaga gatgcacgtc ggtcacctgc gctctaccat
     8761 tattggtgac acagcagtgc gtactctgga gttcctcggt cacaaagtga ttcgcgcaaa
     8821 ccacgtcggc gactggggca ctcagttcgg tatgctgatt gcatggctgg aaaagcagca
     8881 gcaggaaaac gccggtgaaa tggagctggc tgaccttgaa ggtttctacc gcgatgcgaa
     8941 aaagcattac gacgaagatg aagagtttgc cgagcgtgca cgtaactacg tggtaaaact
     9001 gcaaagcggt gacgaatatt tccgcgagat gtggcgcaaa ctggtcgaca tcaccatgac
     9061 gcagaaccag atcacttacg atcgtctcaa cgtgacgctg acccgtgatg acgtgatggg
     9121 cgaaagcctc tacaacccaa tgctgccagg aattgtggcg gatctcaaag ccaaaggtct
     9181 ggcagtagaa agcgaagggg cgaccgtcgt attccttgat gagtttaaaa acaaggaagg
     9241 cgaaccgatg ggcgtgatca ttcagaagaa agatggcggc tatctctaca ccaccactga
     9301 tatcgcctgt gcgaaatatc gttatgaaac actgcatgcc gatcgcgtgc tgtattacat
     9361 cgactcccgt cagcatcaac acctgatgca ggcatgggcg atcgtccgta aagcaggcta
     9421 tgtaccggaa tccgtaccgc tggaacacca catgttcggc atgatgctgg gtaaagacgg
     9481 caaaccgttc aaaacccgcg cgggtggtac cgtgaaactg gcagacctgc tggatgaagc
     9541 cctggagcgt gcacgtcgtc tggtggcaga aaagaacccg gatatgccag ctgacgagct
     9601 ggaaaaactg gctaacgcgg ttggtattgg tgcggtgaaa tatgcggatc tctccaaaaa
     9661 ccgcaccacg gactacatct tcgactggga caacatgctg gcgtttgagg gtaataccgc
     9721 gccatacatg cagtatgctt acacgcgtgt attgtccgtg ttccgtaaag cagaaattaa
     9781 cgaagagcaa ctggctgcag ctccggtaat catccgtgaa gatcgtgaag cgcaactggc
     9841 agctcgcctg ctgcagtttg aagaaaccct caccgtggtt gcccgtgaag gcacgccgca
     9901 tgtaatgtgt gcttacctgt acgatctggc cggtctgttc tctggcttct acgagcactg
     9961 cccgatcctc agcgcagaaa acgaagaagt gcgtaacagc cgtctgaaac tggcacaact
    10021 gacagcgaag acgctgaagc tgggtctgga tacgctgggt attgagactg tagagcgtat
    10081 gtaatcgatt tttcgtgaga gtgaagcctg atcagggtta gccgatcagg cttttttatt
    10141 gccatctaaa tgtattctga aaacggacat gccattgttt tctcactgtt gagtaagagg
    10201 ccagaagcgt aatatccagg cccaggaaaa cgataacggt tcaatttaag gaataccgaa
    10261 gtgtttaaat ttcttgtatt aacattaggc attatctctt gccaggctta cgcagaagat
    10321 acggttatag taaacgacca tgacacttca gccatcaaag attgttggca aaaaaattca
    10381 gatgatgata ctgacattaa cgtgatcaaa tcatgcctgc gacaagaata caatctcgtc
    10441 gatgcgcaat taaataaagc ctatggtgaa gcttatcgtt atatagaaca agtgccacgc
    10501 acaggtgcaa aaaaacttga tactgaacaa cttaacttgc ttaaaaaatc acagcgagcc
    10561 tggctggatt ttagggacaa agaatgtgaa ttaatccttt caaatgagga tgttcaggat
    10621 ttaagtgacc cctattctga atcagaatgg ctatcatgta tgatcataca gaccaatacg
    10681 cgaactcgcc agttgcagct ataccgtaac tctgaagatt tttatccaag ccctttgaca
    10741 agaggataat tcacatcttt ttggcatgtt ttgttgcaag ctattcctga taaatgattg
    10801 caacaaaaca tccagccttt tgactgagtt attaaacata ctcgcgagcg cgtaattttt
    10861 tgtctttcag cgataattca caatcacttc attccgttgt acctttagcg gtggaattaa
    10921 ccgc
//
LOCUS       DAJTQY010000116        10901 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.116, whole genome shotgun sequence.
ACCESSION   DAJTQY010000116 DAJTQY010000000
VERSION     DAJTQY010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10901
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.116"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(152..874)
                     /gene="baeR"
                     /locus_tag="OO349_003382"
     CDS             complement(152..874)
                     /gene="baeR"
                     /locus_tag="OO349_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HCR5868335.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLAYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDVPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            complement(871..2274)
                     /gene="baeS"
                     /locus_tag="OO349_003383"
     CDS             complement(871..2274)
                     /gene="baeS"
                     /locus_tag="OO349_003383"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HCR5868336.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            complement(2271..3686)
                     /locus_tag="OO349_003384"
     CDS             complement(2271..3686)
                     /locus_tag="OO349_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5868337.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHISVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            complement(3687..6752)
                     /gene="mdtC"
                     /locus_tag="OO349_003385"
     CDS             complement(3687..6752)
                     /gene="mdtC"
                     /locus_tag="OO349_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HCR5868338.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLTPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNISIPKTFFPEQDTGVLMGGIQADQSI
                     SFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQQIID
                     RLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRKKLAT
                     LPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIYQPMN
                     QYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGLSAAS
                     TISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILIIAAI
                     ATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLIGIVK
                     KNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSGGDGS
                     ELRQPLEITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            complement(6753..9875)
                     /gene="mdtB"
                     /locus_tag="OO349_003386"
                     /pseudo
     CDS             complement(6753..9875)
                     /gene="mdtB"
                     /locus_tag="OO349_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
     gene            complement(9875..>10901)
                     /gene="mdtA"
                     /locus_tag="OO349_003387"
     CDS             complement(9875..>10901)
                     /gene="mdtA"
                     /locus_tag="OO349_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HCR5868339.1"
                     /translation="YLTGLGTITAANTVTVRSRVDGQLMALHFQEGQQVKAGDLLAEI
                     DPSQFKVALAQTQGQLAKDKATLANARRDLARYQQLAKTNLVSRQELDAQQALVSETE
                     GTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQISSGDTTGIVVITQTHPI
                     DLVFTLPESDIATVVQAQKAGKPLMVEAWDRTNSKKLSEGTLLSLDNQIDATTGTIKV
                     KARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNEGHFVWVLNSENKVSKH
                     LVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVEAQSATTPEEKATSREY
                     AKKGARS"
BASE COUNT         2510 a         3070 c         3003 g         2318 t
ORIGIN      
        1 ctaaaggtgc tgtttactga ttaaagtatg agcgattttc tgatgcctgc cagggcggag
       61 agataatgtc agtttgctga ccatgcgtgg aataatgttg ccggatcggg tctgaacgcc
      121 atctccggct aacaaagtaa tgtcgctaaa actaaacgat gcggcaggcg tcggcttccc
      181 agcggtaacc aacgccgtaa acggcgcgga taaatgactg ttcggcgtcg agagattcca
      241 gcttgcggcg cagattttta atgtggctgt caatggtgcg gtcggttact acgcggtagt
      301 cgtcataaag atgattgagc aattgctcgc gggagaacac ttttcctggt tcgtgagaga
      361 gcgttttaag cagacgaaat tccgcaggcg tcaggtcaag cattttaccg cgccatgaag
      421 cctgaaaacg accttcgtcg ataatcaacg ggctttcggc atcctgttgc tgaagttcgc
      481 gttgcggttt acaacggcgc aaaatggttt tgacacgcgc taccacttcc cgtgggctgt
      541 acggcttgca gatgtagtca tcggcgccaa tctccagccc cagcaggcgg tcgatctctt
      601 cgatttttgc cgtcaccatc acgatcggaa catcggaaaa acgacgaatt tcccggcata
      661 atgtcaggcc atcggtgcca gggagcatca gatccaacag gatcagatcc ggtggtgtct
      721 ggcgcacata cgccagtacc tgatcgccgt ggctgataag cgtcggcgca tagctcgcag
      781 cacgcagata atcaatgagc aactgcccca gcttcggttc atcttccacg atcaaaatac
      841 gcggtgtgtt ttcgtcgatt ggtaactcgg tcatacttct ctctgtaaat cccgttccag
      901 cggtaactct actgtaatgc ttaccccgcc aaaaggcgaa tgggcggcaa taatgcggcc
      961 attatgggct tcaacaatgt tcaggcaaat cgccagcccc agcccggaac cgccgctggc
     1021 gcggttgcgg gaaccttcgg tgcgataaaa acgttcaaac aatttttgta gctgatcgtc
     1081 actgacacct ggcgcactgt cggcaaaggt caggcgcacc gttttgtcgt gctgctcggc
     1141 agagattttc aggctgccgc cgctgtcagt gtagcgcagg ctgttttcca gtaagttatt
     1201 gaataactgc attaaacggt cgcgatcgcc aaatacggtg atactgtctg gcagggaaaa
     1261 ttgcagtttc aggccacggc tggcgaatcg ttcgcgaaat gcgccgcccg ccacttccag
     1321 cagtgggatc aaatctaccg gtgctttttg ataggcgaga gcgccttcat cagacatcga
     1381 caactgatga agatcgtcaa ccagtttggt cagtgtaccg acctccgcct gtaaagacgc
     1441 caccgtctcc ggcgtgaatt tgcgcacgcc atcctgaatg gcttccagtt caccgcgcag
     1501 caccgctaat ggcgtacgca gttcgtgaga aatatcggcc ataaaatcgc gccgcatttg
     1561 ctggtttttc tccagcgtgc tggcgagctg gttgaagtct tgcgccagtt tgcccagttc
     1621 atcttcactg gtgggcgtta cgcgggtagt gaaatcgccc gccgccagtt tgtgcgtgcc
     1681 atcgacaagt cgttttaccg gtgccagcaa accgcgcgcc agcagaaagg tggcgagcgc
     1741 cgcgagtaac gttgccaggg cgacgatcag ccagctggtt tgccgctgtt gtttatcgaa
     1801 attgatatca gtattgcggg tcagccgctc aacaggggag gcaatcaccg cgcccacttc
     1861 cgcaccgttg accagaatgg gtcgccgtgt accgtcaggt ggaatcggcg ctcgcggacc
     1921 aaccagtact ttgttgtttt gatcgaccac ccagaactgg gtacgccagc cgtgcggcgg
     1981 catacccggg ccgggtttat cttccgaatt atcgtgttca aatgaacgca ggatctgaaa
     2041 gacaaagcga tcgttgttgc gcaggaagcg ccagttacca tgctgcgcat actgctcgcc
     2101 aagcgcatca cttaacagct gtaatcgctg ttcattgccg tgtttgatgt aatcaataaa
     2161 gccacgctca aaactgatac gcaccgccca gtgcatactg atcagcaaga caatgcaggt
     2221 ggcgaaaatc gccagaaaca gtttgccggt aataccgggt cgccagaact tcattgcgcg
     2281 ctcctttttc gccgcgaaat agctacattt tgatgcgtat cgttcggcac tctggcaaag
     2341 atgaacgccg gaagggcgat gataaacgcc atgctaagcc aggtgtacat aaagacggtt
     2401 tgtgtggtgc cgctgtcgac gctgatatgc tgcgaaccaa aaagccccag caacagcccg
     2461 gcgatggtga cgccgatact catcgacaat tgcataatca tcgacagcag gctgtttccg
     2521 ctgctcgcca gattgtccgg gagatctttc agcgtcaggg tgttcatgga ggagaaacgg
     2581 gtcgagttga ccatcccttg taaaaacagg acgaacggca aaacgtagta ccagcccaac
     2641 agagcggtag tcataaacaa cagggtgacc agcgacagac ccagcgtggt cgctaccagt
     2701 acccgacgat aaccaaagcg attcaccacc tgtaccacaa ttcgcttcat tcccatgctg
     2761 ccaagcacca tcgggatcat catcagtccg gcatgaaacg gcgagaaacc gaggccaatt
     2821 tgcaggaaaa ccggtgtcat aaagggcaac atgccactgc caatacgtcc ggcaaagctc
     2881 cccgccaggc ccagcgaaaa ggtacgagtg cggaacagtt tcagactgaa cagggcgcgg
     2941 ttgttatttc tggcgtgcag cagataaagc accagtgcca ccacgccaac tgcgaccagg
     3001 ccagcaatcg ccagcggcga taaacctgta cctttactgc cgtccagcgc cagggttaat
     3061 accgccatgc caaccgccag caataaaaag ccggaaagat caaagcgccg cgtctgcatg
     3121 gtgtagttcg gcattaacat caatgtggcg atcgcaccga taatccccac cggaatgttg
     3181 atcaaaaaga tccagtgcca cgacgcgtat tctaccagca gaccgccgag cgccggaccg
     3241 agcagcggac cgacctgacc gggtaacgtg acaaaggtca tcgccgccat atattgctcg
     3301 cgcggtacga ttttcatcac cgtcaacctg ccgaccggca ccatcatcgc accgccaacg
     3361 ccctgtaacg cgcgtgccag caacagttcg ttcagcgtgc cggaaagcgc gcaaaacagt
     3421 gaaccgagag taaacagcac gatggcggta aagaaaatat tgcgcacgcc gactttgtcc
     3481 gccagccagc cgctggcggg cagcatcacc gccacggtca gcacataaga gacaatgacc
     3541 atgtgcatat gcaacggact ttccccgagg ctttgcgcca ttgagggaag ggcggtgttt
     3601 acgatggtgg tgtccagcga ctgcataaag aagccgaaag ccacaatcca taattgccag
     3661 cgggtgctgt cgggaagatc tgtcatttac tcggttaccg tttgtttagg tttacgcgaa
     3721 aaacgcagcc gcagacggtc gaaaaagaga tacaccaccg gcgtggtata cagcgtcagg
     3781 agctggctca tcaccagacc gccgacaatg gtgatctcca ggggttgccg cagctccgag
     3841 ccgtcaccgc ccgacaatac cagcggcagc gcgccaaaca gcgccgccag ggtggtcatc
     3901 ataatcgggc gaaaacgcag cagacaggcc tggaaaatag cttcctgcgg cgtcaggtta
     3961 ccgtgccgtt gggcttcaag cgcaaaatcg accatcataa tggcgttttt cttcacgatg
     4021 ccgattaata gcatgatccc tatcagggcg attaggctga acggggcatt gaacagctcc
     4081 agcgccaaca gcgctccaac gcccgccgag ggcagggtgg agagaatcgt cagcggatgt
     4141 acgtaactct cgtaaaggat acccagcacg atatacaccg tggcgatggc ggcgataatc
     4201 aggatcacct gcgagttcat cgtctcctgg aaaacctgcg ccgtgccggc aaaactgccg
     4261 cgcaccgtcg aaggcacacc aagctgggtc attgcgcgat cgatcgccgc actggcgtcc
     4321 gagagcgatt ttccggtcgg caggttgaac gagatggtcg aggccgccga taatccctga
     4381 tgattcaccg atagtggggc attcgccggt tgccatttag cgaaatacga cagcgggatc
     4441 gctttgcctt cgttattgat aacgaacatt ttttccagcg cactgatgtc ctgggtatag
     4501 cgcggatcca cttccatcac cactttatac tggttcatcg gctggtaaat ggtcgagatt
     4561 tgccgctgac cgaaggcgtt atttaacaga ctgttggcgg cctgtacgtc gattcccagc
     4621 cgtgccatgg tgtcgcggtc gtaaaccaga ttcatctccg cgccgttatc ctgctgatcg
     4681 gagttcacgt ccgccagttc cggcaacgtc gccagttttt tgcggatttt cggctcccat
     4741 tcgcgcagtg ccgccaggtc gtcggataac aacgtgtact ggtagctggc gttcgactga
     4801 cgcccaccaa cgcgaatatc ttgtaccgcc atcaggaaca gattcgcccc cggttctttc
     4861 gccagcttca cgcgcagacg gtcgataatt tgctgcgccg tttcgctgcg ttcgtcgcgt
     4921 ggcttgaggg tgataaacat catcccactg ttaactcgtg aaccgccggt aaagccggtg
     4981 acattatcca ctgccggatc gtcacggata attttcatga aatcctgcaa tttgccgcgc
     5041 atcgcctgaa acgaaatact ctgatccgcc tgtatcccac ccatcagtac gccggtgtcc
     5101 tgctccggga agaaggtttt cgggatcgag atattcagcg caatggtgcc aagcagcacc
     5161 acgcccacca gacgggtatg attgagcacc catttcagcg acttgccgta gccttgttgc
     5221 agggctacca acatgcggcc aaaaccacgc agtcgctttt gctcacgcgg cttgctggct
     5281 tttagcatcc agccgcacat cattggcgtt agcgtcaggg aaaccagcaa cgaaatacca
     5341 atggcgacag aaagcgtcac ggcaaactcg cgtaacagtc ggcccggcaa tccgcccatc
     5401 aacagcagcg gcaggaacac cgccaccagt gacagactca tcgacagcac cgtaaagccg
     5461 acttcgcgag taccttgcag cgcggcctgt agtggtttca tccccgcttc cagatgacgg
     5521 gcaatgtttt ccagcaccac aatggcgtca tccaccacga aaccagtagc aatggtgagc
     5581 gccatcagcg aaagattatt gagactgaat ccgcacaggt acatcgccgc aaacgtacca
     5641 atcagcgaaa ccggcaccgc aacggcggga ataatagtgg cgcgacccga gcgcaggaat
     5701 aaaaagacca ccagaatcac cagcgccacc gagataatca gcgtttgctc gacttcttcc
     5761 agcgaggcgc gaatggtggg ggagcgatcc tgggcaattt gcagatcaat cgccgccgga
     5821 atggtctcct gcaactctgg taattttgcc cggatgctgt ctaccgtctg gataatattg
     5881 gcttccggca gtttgcggat catcagtaaa atagccggtt tggcgttggt catcccggcg
     5941 ttgcgcacat cctgcactga gtcagtcacc gtcgccacat cgcccagacg aaccgcgccg
     6001 ccgttgttgt agtgaataat cagcggctga tattcagcgg cggttttcag ttcatcgttg
     6061 gtctggatct gccagcggtg agtgccatct tccagcgccc cctgcggttt acgcacgttg
     6121 gcattgctga tggcagtgcg tacgtcgtcc agcgacacgc cctgattaaa cagcgcctgc
     6181 ggggtcagcc cgacgcgcac ggcgggcagc gagctgccac cgacatcgac atcaccgaca
     6241 ccgtcgattt gcgaaatcgt cggagccagc tgcgtcgagg cgaaatcgta cagttcaccc
     6301 tgcgaataag tatcggatgt cagcgtgagg atcataattg gcgcatccga cgggttcgct
     6361 ttgcgatagg tcgggcggct gggcatcccg ctgggcagca aactttgtgc agcgttgatc
     6421 gccgcctgca catcacgcgc tgcgccgttg atatcccggt caaaatcaaa ctgcaaaata
     6481 atgcgcgtgc tgccgagcga actgctggag gtcatttcac taaccccggc aatgcgccca
     6541 agtgagcgct ccagcggcgt ggcaacggaa gacgccattg tctccggcga cgcaccgggc
     6601 agcgaggcgc tgaccataat caccggaaaa tcgacctgcg gcagcggggc gaccggcagc
     6661 atacggaaac ccagtatgcc gcacagggta atggcgaccg acagtaaaat cgtcgccacc
     6721 gggcggtaaa tgaagagggc aaaaaacttc acttacgcct cctcttcatg gcgagcaaag
     6781 cgacttttgg tccacaatgc caggcggtcg aacagcagat aaatcaccgg cgtggtgaac
     6841 agcgtcagca cctggctgac aatcagaccg ccgaccatgc cgatacctaa cggacgacgc
     6901 agttccgcgc cgaccccggt actcaacatc agcggcagcg cgccaagcag agccgccagg
     6961 gtggtcatca ggatcggacg aaaacgcaac agacaagcct ggtagattgc atcgcgcggc
     7021 gacatgcctt gctcgcgctc agcagccagc gcgaagtcga tcatcatgat ggcgttcttc
     7081 ttcacaatac cgatcagcaa aataatgccg ataatcgcaa tcacatccag ttcgctaccg
     7141 gcaatcatta atgccagcag cgcgccgacc cctgcggtgg gtagcgtcga gagaatggtg
     7201 atcgggtgaa taaagctctc gtacagaatg ccgagcacga tatacatcgc caccaccgcc
     7261 gcgacaatca gccagacagt gctgcccagc gccgactgga aggcgagggt gctgccctag
     7321 aattgcgtgg tgatatccac cggcagattc agcgtctttt cggtgtccat aatcgcctgc
     7381 accgcatcgc ccagcgaata gttatccggc acgttaaagg agatggttgt taccgggaac
     7441 tgatccagat ggttgatgga aagcggcgca aaacgctgct caattttggc aattgagctt
     7501 aacggcacca cgccaccgtc gctgctggtc aggcgaatgg tatccagcgc cgcgaggcct
     7561 ggggtatttt cggtattgtg ctccagcacc acgcgatact ggttagcctg agtataaata
     7621 gtggaaatca gtcgctgacc aaacgcgttg tacagggcgt tatcgacgtc cgccatgctg
     7681 atccccagac ggctggcgct gtcgcgatca acattgacat acgccaccag ccctttgtcc
     7741 tgccagtcgc tggagacatc agaaagctgt ggcagttgct ggagtttttc catcaactgt
     7801 ggcacccagg tactgagcgc atccagtgac gtggcctgca aggtaaactg gtactgggtg
     7861 cggctgacct gagtatcgat ggtcaggtcc tgcgttggtt gcaggaagag atcgacgccc
     7921 ggcactttat ctaccgccgt ttgcagacgg gcgatgactt tttgcacccg atcatcacgt
     7981 tcatccaacg gtttgaggtt gatttgtaaa cgtgcactat tcagcgacgg gttagtgcca
     8041 tcaacgccaa caaatgaggt caggctttgc actgccggat cctgcaaaat cacgtccgcg
     8101 acctggcgtt gtcgctgggc catattggca aaggagctgg attgcggtgc ctgcaaagtg
     8161 ccctgaataa tgccgttgtc ctgtaccggg aagaaacctt tcggaatgaa cacccacagc
     8221 agcacgctaa gcagcagcgt gctgagtgcc acgcttaagg tcagccacgg atgattcagc
     8281 actttcgcca gtccacgacc ataggcggcg attatcctgt cgaacatttt ttccgaggca
     8341 cgggagaagc ggttctgttt acgcaacgac tcctggctga gcatccgcgc gcacatcatc
     8401 ggtgtcaggg tcagcgacac caccgctgag atcaaaatcg ctaccgccag ggtaatagca
     8461 aattcgcgga acagtcgccc gacgatatcg cccataaaca gcagtgggat caacaccgca
     8521 atcagtgaga aggtcagcga gataatggta aagccgattt cacctgcgcc cttgagcgcc
     8581 gccgccaacg gtttttcgcc tttttcgata tagcgggaaa tgttttcgat caccacgatg
     8641 gcgtcatcga ccacgaatcc ggtggcgata gttaacgcca tcagtgtcag gttattgatt
     8701 gaaaaatcga gaaacaccat aaccgcgaaa gtgccgatta acgacagcgg tacagcaaca
     8761 ccgggaatga tggtcgccgg aatattgcgc aaaaacaggt agataatcat gactaccagc
     8821 gcgatagcca tcatcaattc aaactgagta tcatcgacgg atgcgcggat attggtggtg
     8881 cgatcggaaa gcactgtcac cttcaccgat ttcggcagac tctcagtgag ctgtggcagc
     8941 atctgccgaa tgctgtcggc ggtggagata atgttagcac cgggctggcg ctgaacattc
     9001 atcacaatgg cctgttcttt gttcgcccac gcgccgagcc agctgttttc tgcaccttgc
     9061 tctacagttg cgacatcgcc cagacgaatt ggcgcgccgt tctggtaggc gatgattagc
     9121 tggcgatact cttcggcgga ttgcatctgg tcgttcgcgg aaagcgtgac cgcacgggaa
     9181 gggccgtcga ggctaccttt tgccgagtta acgttagcgc cggtaatggc ggtgcgcacg
     9241 gtttcgctgg tcaggccgag ggcggcaatc gcctgagcgt taagtttgac gcgaacagcc
     9301 ggacgctgac cgccggaaag cgtcaccagg ccgacgccgg aaatctgcga gattttctgc
     9361 gcgacgcggg tttccaccat atcttccact tgcgtcatcg gcatggcggt tgaggtgacg
     9421 gcgagcgtca tgatcggcgg atctgccggg ttcactttgc tgtaaaccgg cgggttaggc
     9481 agatcgctcg gcaacaagtt ggtcgcagcg ttaatcgcgg cctgcacttc ctgctcggcg
     9541 acattgagcg gtaatgttag ctggaactgc aaagtgataa ctgacgcacc gccggaactt
     9601 tgcgacgaca tctgtttcag gccagacatc tgcccgaact ggcgttcgag cggcgcggta
     9661 acggcagagg tcatgacatc cgggctggca cctgggtaga gcgtgaccac ctgaatggtc
     9721 ggatagtcca cttccggcag cgccgaaacg ggcagggcgc gataaccgat aatcccggcg
     9781 agtaagatcg ccaccatcag cagcgtggtg gcgacaggac gcataataaa caggcgcgac
     9841 gggccgcctg tgctgctcgg gggtaacacc tgcatcagga gcgtgctcct tttttcgcgt
     9901 attcgcggct ggtggctttc tcttccggag tggtggcgct ctgggcttcc accacttcca
     9961 ctttcgcccc ttcggtcagg cgatcaatgc cgtctgtcac cacgcgatcg cccgcagaaa
    10021 tacctgcacg gatcaccact ttctgactgt cctgaatgcc cggcgtcacc agatgtttgc
    10081 tgaccttgtt ttcgctattc agcacccaga caaaatggcc ttcattgccc atttgcaggg
    10141 cggctgttgg gatcactacg gcgttttgtt cggtgtcgac taacatgcgc gcgttaacaa
    10201 actgattggg aaacagcgca tcatcctgat tattaaagcg tgctttcact ttaatcgtac
    10261 cggttgtggc atcgatttgg ttatcgaggc ttaacagcgt gccttcactt aatttcttcg
    10321 aattggtgcg atcccaggct tctaccatca gcggttttcc ggctttttgc gcctgcacta
    10381 cagtagcgat atcgctttcc ggcagggtaa agactaaatc gataggatgc gtctgggtga
    10441 tcaccacaat tccggtggta tcaccactgg agatttggtt accaacatca acttgcttga
    10501 gaccaacgcg accatcgact ggtgcggtga tgcggctcca gtcgagttgc aactgcgcgc
    10561 tggcgacgct tgcttcatca gccttaatgg tgccttcggt ttcactgacc agcgcctgtt
    10621 gggcatccag ctcctgacgg gagacgagat tggtttttgc cagttgctga taacgcgcca
    10681 gatcacggcg ggcgttagca agcgtggctt tatcttttgc cagttggccc tgggtttgtg
    10741 ctaatgcaac tttgaactgg ctggggtcaa tttctgccag taaatcgcct gctttgacct
    10801 gctggccttc ctggaaatgt aacgccatca gttgaccgtc cacgcggctg cgcaccgtaa
    10861 cagtattagc ggcggtaatg gtgccaagcc cggtgaggta a
//
LOCUS       DAJTQY010000117        10756 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.117, whole genome shotgun sequence.
ACCESSION   DAJTQY010000117 DAJTQY010000000
VERSION     DAJTQY010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10756
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.117"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(160..432)
                     /locus_tag="OO349_003388"
                     /pseudo
     CDS             complement(160..432)
                     /locus_tag="OO349_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300094.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
     gene            complement(526..1088)
                     /locus_tag="OO349_003389"
                     /pseudo
     CDS             complement(526..1088)
                     /locus_tag="OO349_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004714555.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
     gene            complement(1292..1498)
                     /locus_tag="OO349_003390"
     CDS             complement(1292..1498)
                     /locus_tag="OO349_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HCR5868340.1"
                     /translation="MVYKSDSDELAVLRAENTRLVSLLEAHGIEWRRKPQSPVQCVFV
                     LSTDEKVALFRRLFRGRDDVWALR"
     gene            complement(1605..2168)
                     /locus_tag="OO349_003391"
     CDS             complement(1605..2168)
                     /locus_tag="OO349_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HCR5868341.1"
                     /translation="MYNANPNYKMDFMILKDVNEHMDGMFQRFSKLLPFRIDFAYRKD
                     TPSFGHSCKHSMCMEIYRLLSETQTMLAGYYWVMEYTPDKGLHIHFIGYLDGQRHKNS
                     YQISRQLGDIWRRITEGEGYFHLCRAKDKYPVRIDHVIHYSDKSAVDDLRYALSYLAK
                     QDQKEHGIILGRSRLPEKSNRGRPRHN"
     gene            complement(3543..5378)
                     /locus_tag="OO349_003392"
     CDS             complement(3543..5378)
                     /locus_tag="OO349_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5868342.1"
                     /translation="MRTFTNMLYDICTVLGLFKEGENPAHKRKSTNFEMHQKFWDQRY
                     NEISRIIDAEGVFSQEQRRIIYARYEHFYYMMNSYPVHSTLKPEFLRSYCLRTFGVIF
                     LVVDMYNTYRPENDSAFYYHIYNFLQKSYCPCLDHADTESDEAAVKRYLREYLAELGF
                     NKEDFHENGKLYALGKYTGTIRKDNGKSKSLMQQYIMAIKNEYKKDYREKKLDKDELE
                     KVLRNIDKFYNAFYSLSVLLDIQRKTKILQSLAYYLRVLVREGLWIHGLYGYAAQYLY
                     DFTSFDTTPYAKKLLEMFYKFQNSAEGTLSRYSVSLDDKSQEYIFRLKDLVFNINDKN
                     GCDDAYLKKIISYFGQLQNEAVHVTSCYETLAVYICLIRKNKINDVLQHYDDMERKGL
                     FGELPSGYVRGALSLLRTALEVKVNRKNIKYGSLFYWLDHVKAYQDAFIEKIPLIDPV
                     YKEGEIQYDANNFTLMRVIKMYNCMLEKISTKPYIAPPYITGLLDDVEKVLDKINILI
                     DKEYVYDGKTLAEVIMENKVLSSRERKETMIGLFTGSKKYTLLQCVEKLGVLVHYVKS
                     PVDEIKNVMMLYGDKAENRNRRRMIYDALTIICEDDIRNNPPELS"
     gene            5479..5766
                     /locus_tag="OO349_003393"
     CDS             5479..5766
                     /locus_tag="OO349_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000070930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCR5868343.1"
                     /translation="MSNQTGQLYTPSRILRMAELTRLLGISRSTIYVKINPASKYHDP
                     TFPKPIRLGSASVGWRASAIDEWLMLHTAPAWSEPENKLKELNDDDKTITP"
     gene            5738..7267
                     /locus_tag="OO349_003394"
     CDS             5738..7267
                     /locus_tag="OO349_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HCR5868344.1"
                     /translation="MMTTKLSLPELDTTKLPDRCQALLDEMHEETGIRREILLSVMLT
                     VMAASVQDTHEVELSGGQRTSLQIFMCLSSASGSGKTSACAKLIAPVHETEEELHQAY
                     IDDKKNYDRMMEMWTTDKKILERRYKKEIERSPENAAAARAALEKCIANKPEPPVQQV
                     LIVNDATPEGIALKLSQSPSLLLLSDEGGTILDKRFERKSALYNTLWSGQPVNVERAS
                     RPGFRLNDVRLTILILTQPVIFNKFFTLTGDQIRGNGFLARLLFCEPGAIKIMTTEPH
                     TATPVVTQQCASCFRKKSFGSQIRDSLRASRERRAKSKERICMTLSNAASRALERFHK
                     ENMDTVRQNPHMGTFEDIIVRKREQVVRIAALLELEKDPDSTVITLESTNSAIYLIEF
                     YFKHLIYKLESLREISPAEKLDKWLQKRIITTAGYIFQKSYILQYAPYALRKKCVLDE
                     ALDILAEQRKIRIDDNLVVYIGNTITPSELAKKLNIPAFDAGVFICDHQNILKYHRNR
                     L"
     gene            complement(7437..8654)
                     /locus_tag="OO349_003395"
     CDS             complement(7437..8654)
                     /locus_tag="OO349_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000279856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5868345.1"
                     /translation="MAVLTDTKARHIKPDDKPLPHGGITGLTLHPSSVKGRGKWVFRY
                     VSPVTQKRRNAGLGTYPEVSIAEAARTARIMREQLAAGDDPLEIKKAESEKVVIPTFA
                     DAARRVHAELSPGWENPKHVRQWLSTLENYAFPQLGAKTLDSITAADVAETLRPVWLT
                     LSETASRVKQRIHVVMQWGWAHGFCVANPVDVVDHLLPQQTRGRDEHQPAMPWRQLPL
                     FVATSVYSDEPYNVTRALLLMVILTATRSGEARGMRWAEIDFHKRVWTIPAERMKARL
                     QHRVPLSRQAIYILENIRGLHDELVFPSPRKQQILSDMVLTSFLRKKKAVSDIPGRVA
                     TAHGFRSTFRDWCSEQGYSRDLAERALAHTLKNKVEAAYHRTDLLEQRVPMMQAWADY
                     VMSQLAKNTKEFA"
     gene            complement(9021..10007)
                     /locus_tag="OO349_003396"
     CDS             complement(9021..10007)
                     /locus_tag="OO349_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5868346.1"
                     /translation="MIPDYLTFIRFQDKRNLIYIYAIGLILIGFYWKNAGFTFPSEDI
                     GVVSGILALVLYNFIFDLKAYWAYKCVTKNIDFSWFKKKQNHKIELFLTQPLVAGFLS
                     LIMLSAMSWGLYQLLPSLYALFLISLLGPLVIFLLFRMIRTSYVKQVAISVAKKVKYK
                     SLTRYVLLSVCISTVVNLLTISPLRNSDSFVTEGQWLTFKSIIALLILCGVVLAINLF
                     FLRFSKRPAFLGRFFLQEIDLFFSSENTLSTFFAKPLWLRLFILRVIEMMWITLVSVL
                     ATLVEWRIWFEAYFLLCYVPCLIYYFFHCRFLWHNDFMMACDMYFRWGHFNK"
     gene            complement(10004..10532)
                     /locus_tag="OO349_003397"
                     /pseudo
     CDS             complement(10004..10532)
                     /locus_tag="OO349_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309324.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
     gene            10599..>10756
                     /locus_tag="OO349_003398"
     CDS             10599..>10756
                     /locus_tag="OO349_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5868347.1"
                     /translation="MTKTVSTSKKPRKQHSPEFRSEALKLAERIGVTAAARELSLYES
                     QLYNWRSK"
BASE COUNT         3349 a         2470 c         2038 g         2899 t
ORIGIN      
        1 acgtgggtgg actggtataa caatcgacga ttgctggaaa ggctgggcca tactcctccg
       61 gcagaagcag aaaaagctta ttatgcttcc atcggaaacg atgatctggc agcctgagtt
      121 cacagataaa acactctcca ggaaacccgg ggcggttcac ttacaccaga catcgttatt
      181 aagtagcagc accataagat aaggagtggc atcgttagcc acagcttccc tactccagag
      241 gtaatataaa ggggtgggct cgactgattt atctttacgt cgcttacact gcaaatgttc
      301 agaaattagt ctatgcagct caccagtaaa atcagcaatc agagaaggaa ttgccttatt
      361 cataccaggg caaggtatta atttaaactg taataattta attttaggat gtgtagcagc
      421 agcccgatac agagttgcaa ggacacactt ttgccagagg gcgttacagg aaagcttaat
      481 gtttgattct gtatacataa taaatcacct tatagttaca ataggtcaaa aaccgctgta
      541 gccaaagtta cgctggcctg atgctttagt gtcgagcttc gtcaaataat ccagacgtac
      601 caataagcgc tggtactgct ctgggaaatt aggatcgtta atatccagga tgtaacgtcc
      661 attagcaggg aaatgaacta atccctggtg atcttccagt tccaacccta aggcactgta
      721 ccaggctccg gtaattaacc ctctcagggt gtcagagcgc tcatagtttc ccagatggta
      781 ataggcgtct ttgttaaaaa aaggcagacg tgataatgac atttaaagga ctgcgtatat
      841 tctttcgccc agataataca aaccgacgtt gcgtaggtgc gtttgccttg ttgtttttta
      901 cggtgttgat ctgcattaat tttcgcattc agcgagttac ggaaacgaga tatctctccg
      961 gggatcaggt tagggaaaca ggtaatgata tccccgttgt caatcacagg agggtaatgg
     1021 agatcccccc gtattgctgt tattctggaa gtgtttttaa tattcgtatt gatagttctc
     1081 agagatatat cacactctag atattttaat taagtatcag ttcgatatcg gcatattgta
     1141 cactacctca ggtagatgat gagatcagat acagggatca actgacggtg agcgcagtcc
     1201 ccacatttta tccagggctt actgcatatt cccaacagcc attcgttagc caggtagacg
     1261 aataccctga tttgccgctg gttttgcttt cctatcgaag ggcccataca tcatcacgcc
     1321 cacgaaacaa ccgacgaaat aacgcaactt tctcatccgt ggataatacg aaaacgcact
     1381 gcacagggct ctgcggttta cgtcgccatt caatcccatg agcttcaagt aatgagacca
     1441 gccgggtatt ttctgcccgc agtactgcta gttcatcgct gtcagattta taaaccatat
     1501 gcgttatcac ttgtacctgt ctcccggcgg caccacgact gaaattcgtg tgaaggcaaa
     1561 tccacgcgat aacttacgat agttcggaca taagccccca taattcagtt atgtcgtggt
     1621 cgaccccggt tacttttttc gggaagcctg ctacggccca gaataatacc gtgttctttc
     1681 tgatcctgtt tagccagata gctaagggcg taacgtaagt cgtctaccgc agatttatca
     1741 ctatagtgaa tcacgtgatc gatacgcacc gggtatttgt ctttagcccg gcacagatga
     1801 aaataaccct ccccttccgt gatccgcctc cagatatctc ccagttgcct ggatatctga
     1861 taagagttct tgtgacgttg tccatccagg tagccgataa aatgaatatg aagccctttg
     1921 tctggtgtat attccattac ccagtaatat cccgccaaca tcgtctgggt ttcgctaagt
     1981 aaacggtata tttccataca catactgtgc ttacaggaat gcccgaaact gggcgtgtct
     2041 ttcctgtaag caaaatcaat tctgaaaggt aacagtttag aaaaacgttg aaacatacca
     2101 tccatgtgtt cattcacgtc tttcagaatc atgaaatcca ttttgtaatt gggattggca
     2161 ttatacattt tatagtcctt actttataaa actcacagga agagagaatt acgtaaatac
     2221 tgagagtaga gaaacgccgt accacggcat taataaacga agtaaaccag cagcacagac
     2281 aacctgataa aactgttttg tattaccgca ttactgtgat gaggtaatac ttcagaatac
     2341 agtgagactc tcttaaactg agaaccagta cgaattacaa tggaaaaaca cacctcatag
     2401 aattgagttt caggaagcac cactaaattc tttacaggaa gtaatctgac attcacaaaa
     2461 ctcagtatcg gctagctaca ggcaacatcg ctattaatat tgtcgaacca ttaagtaaag
     2521 ccgagagaga ccacgggtat ttaatcatta gcataaaaga ataatggtat acagaacata
     2581 cccacacacc ataagaagat tgttccatac agaaaagatc atatttacct atacctataa
     2641 actgtaacgg agtcataaca cgctctaata gtgttccgat acgtaaccag tgttaagtac
     2701 gtacaggaca acattacaca cataattcac catacagctt cccccatatg cttcactgcg
     2761 tagtgtatgg agatatatgc attacacaca tacagaatac ttagcataaa gaggagtgac
     2821 attaccacgt aaagagataa gttatacaac aaacatacaa acaatacggc acagacacac
     2881 aataccataa ctccaactaa taatactaat taaccttaat tatttatggt atatgaaata
     2941 aatgtgattc tcccggaact aactaaccta accagacaga tgatcactgt ataaaaactt
     3001 cagttaatac tcatttgttt tgtcgactca gtatataatg agtgaccagt aatcaggata
     3061 atcacactta cataacctgt aaaaaagaac aagaagaata agccactaac ataatgcaaa
     3121 atggcatctg aatcgatcgc agtttggcat tcgaatcgat catgatttgg cccaatccgt
     3181 gatcgaatag agcggaatac gcaacataat tcaccagaca agagcttaca ccgaattaca
     3241 gattatgtat taatgtcaaa actggcatat tttacagata caatgaacat gcaacccatc
     3301 aatctaatag gggcggtatg tacatctgcc catgaatggg atggcgctaa gaatacaaaa
     3361 caattagaca tagatcacaa aaacacacca ttctaattga aaaacaaaac aaacaaataa
     3421 atcatactat tatagaattt tctcttgcca gacatagata atacccctgc taattttgct
     3481 aatggttagc aaaattagca gggtatataa agattattga cccaaaacac actcagatga
     3541 attcacgaca gctcaggagg gttattcctg atgtcatctt cacaaataat ggttaacgca
     3601 tcatatatca ttctgcgtct gttcctgttt tcggccttat cgccatacaa catcatcaca
     3661 tttttaattt catcgaccgg tgatttcaca tagtgaacta atacaccaag cttctcaaca
     3721 cattgcagta aagtatattt cttactgcct gtgaaaagcc ctatcatagt ttctttacgt
     3781 tcgcgactgg ataatacttt gttttccata atgacttctg ccagcgtctt accatcataa
     3841 acatactctt tatctatcag aatatttatc ttatccagca ccttctctac gtcatcaagc
     3901 aaacctgtaa tatatggggg cgcaatatat ggtttcgtac tgatcttttc aagcatacaa
     3961 ttatacatct taataacacg catcagggta aaattgttcg catcatactg tatttcaccc
     4021 tccttatata caggatcaat aagtggtatt ttttcaataa atgcatcctg ataggctttt
     4081 acatggtcaa gccaataaaa caacgagcca tactttatat tttttctatt tactttaacc
     4141 tccagtgctg tccgaagcaa cgataatgcc cccctgacat atcctgaagg taattcacca
     4201 aacaatcctt ttctttccat gtcatcataa tgctgcagca catcattaat tttatttttc
     4261 cttatcagac aaatgtacac cgcaagtgtt tcgtagcagc tggtcacatg aacagcctca
     4321 ttctgcaact gcccaaaata gcttataatc tttttcagat atgcgtcatc acaaccattc
     4381 ttatcattaa tattaaatac aagatctttc agacggaaaa tatattcctg agatttatca
     4441 tcaagtgaaa cactataacg agacaatgtt ccttctgcac tgttttgaaa cttataaaac
     4501 atttccagta atttttttgc ataaggcgtt gtatcaaagc tggtaaagtc ataaagatat
     4561 tgcgcagcgt aaccatacaa accatgaatc cagagtcctt ctctcaccaa cactctgaga
     4621 taatatgcaa ggctttgaag tatcttcgtc tttcgttgta tatcaagtaa gacacttaat
     4681 gaataaaatg cattatagaa tttatcaata ttcctgagca ctttctccag ttcatcctta
     4741 tccagcttct tctcccggta atccttttta tattcatttt tgatagccat gatatattgt
     4801 tgcatcaggc tcttgctttt cccgttatcc ttccgtatag ttcctgtata tttccccaga
     4861 gcatacagct ttccattttc atgaaaatct tccttgttga atcccagttc agccagatat
     4921 tcccgaagat aacgtttaac agcagcttca tcgctttctg tatcagcgtg atcaagacaa
     4981 ggacaatacg atttctgtaa aaaattataa atatgataat aaaaagcaga gtcattctct
     5041 ggtctgtatg tattatacat atctacaaca agaaaaataa ccccaaaagt gcgcagacaa
     5101 tagctgcgaa ggaattcagg ttttagtgtg gaatgcactg gataagaatt catcatgtaa
     5161 taaaaatgtt catatcgggc atatatgatc cgccgttgct cctgactgaa caccccttca
     5221 gcatcaataa tacgactgat ctcattataa cgttgatccc agaatttttg atgcatttca
     5281 aaattggtac tcttccgctt gtgtgccgga ttctcgcctt ccttaaacaa ccccaaaaca
     5341 gtacaaatat catacaacat atttgtaaat gtgcgcatat gctgtgctct ccggttggtt
     5401 tgcattttat cgcagaggac attctgagct taacgtatca aactgacaca acgacatgca
     5461 atgcatatgg agaacaatat gtcaaatcaa accggacagc tttacacgcc ttcacgcatc
     5521 ctgcgtatgg ctgaacttac ccgactgctg ggtatatcaa gatctactat ttacgtgaaa
     5581 ataaatccgg cctcgaaata ccacgaccct acctttccca aaccaataag gcttggtagt
     5641 gcatccgtag gctggcgggc atctgccatc gatgagtggc tgatgttaca tacggcacca
     5701 gcctggtctg aacctgaaaa caaactaaag gaacttaatg atgacgacaa aactatcact
     5761 ccctgaactt gacacaacta aactgcctga caggtgccag gcattacttg atgaaatgca
     5821 tgaagaaaca ggaatacgcc gtgaaatact tctgtctgtt atgctgacgg tcatggctgc
     5881 ctcggtccag gatacgcatg aagttgaact ttccggaggg caacgtacca gccttcagat
     5941 atttatgtgt ctttcatcag catcaggcag tggaaaaacc tctgcctgcg caaaattaat
     6001 cgctcctgtt catgaaacag aagaagaact acatcaggca tatatcgacg ataaaaaaaa
     6061 ctatgaccga atgatggaaa tgtggacaac agataaaaaa atcctggagc ggagatataa
     6121 aaaggaaatt gaaagatccc cggaaaatgc tgctgctgca cgagcagcac ttgaaaagtg
     6181 tatagcaaat aaacccgaac ctcccgtaca acaggttctc atcgtgaatg acgcgacacc
     6241 agaagggata gctttgaaac tcagccagtc accctctctt ctgttgctgt ctgatgaagg
     6301 aggtactatt ctggataagc gtttcgaacg caaatcggca ctgtacaaca cattatggag
     6361 tgggcaacca gtcaacgttg aacgggcatc cagacccggg ttccggctga atgatgtccg
     6421 tctcactata cttatactga ctcaaccggt aatatttaat aagtttttta ctctcactgg
     6481 cgatcagatc cggggcaatg gctttctggc ccggttgcta ttttgcgaac ccggagcaat
     6541 taaaataatg acgacagaac cgcatactgc cactcctgtt gtgacgcaac aatgtgcaag
     6601 ctgttttcgt aagaaaagtt ttggcagtca gatcagagac tctctgagag catccagaga
     6661 aagacgtgca aaaagtaaag agcgcatctg catgacatta tcaaatgcag catcccgcgc
     6721 actggagcgc tttcacaaag aaaatatgga tactgtcaga cagaatcctc atatgggtac
     6781 atttgaagac attattgtca gaaaaagaga gcaggttgtc aggatagcag cacttctgga
     6841 actggagaaa gatccagact cgacagtaat aacgctggaa agtactaata gcgccattta
     6901 tctcattgaa ttttatttca agcatcttat atataaactt gaatcccttc gggaaatatc
     6961 cccggctgag aaacttgata aatggcttca gaaaagaatt atcacaacag caggttacat
     7021 attccagaaa agttatattt tacaatatgc cccatatgct ctcaggaaaa aatgtgttct
     7081 ggatgaagca cttgacatac tggcggaaca aaggaaaata aggattgatg ataacctggt
     7141 tgtttatata ggaaacacga ttacaccatc cgaactggca aaaaaactaa atattcctgc
     7201 attcgatgcc ggagttttca tttgtgatca tcaaaacata ctgaagtatc accgaaacag
     7261 actatagata ctatcggaca aaaaataaat tatcacaagt gtattattcg ttataataat
     7321 gaataatcgt tatctgagtg caatgccagg ttttcattac gaactggcat tgcatttatt
     7381 tttcaaaaaa gaaagcagta accatttggc tactctgagt atgccagtcc atgaaactaa
     7441 gcgaattcct tagtgttttt agcaagttga gacatcacat aatccgccca tgcctgcatc
     7501 atcggtacac gctgctccag tagatcagta cgatgatatg ccgcctcaac cttatttttc
     7561 agcgtatgag cgagcgccct ttccgccaga tcccgcgaat acccctgttc actacaccag
     7621 tccctgaatg ttgagcgaaa accatgtgcc gtggcaactc gccccggaat gtcactgacg
     7681 gctttctttt tacgcagaaa acttgtcaac accatatcgg aaaggatctg ctgctttctg
     7741 ggtgaaggga acaccagttc atcatgcagg ccacgtatat tttccagaat gtaaatagcc
     7801 tgccgggata aaggaacacg atgctgtagc ctagctttca ttctttctgc aggtatagtc
     7861 catacccgct tatgaaaatc aatttcagcc cagcgcattc ccctggcttc gcccgagcga
     7921 gttgctgtaa gtatcaccat taataacagt gcgcgggtaa cattataagg ttcatcacta
     7981 tacacactgg tcgccacaaa aagcggtaac tgcctccagg gcattgcggg ttggtgttca
     8041 tcacgtcctc ttgtctgctg aggaagcaaa tggtcaacca catcaacagg atttgccaca
     8101 caaaaaccgt gcgcccatcc ccactgcata acaacatgaa tgcgctgttt aacccggctt
     8161 gccgtttctg acaaggttaa ccagactgga cgcagtgttt ctgccacgtc cgcagccgta
     8221 atcgaatcca gcgtttttgc tcccagttga ggaaacgcgt aattctcaag cgtcgataac
     8281 cactgcctta catgctttgg attctcccat ccaggagaca gttctgcatg tacacgcctg
     8341 gctgcatcgg caaatgttgg gataacgact ttctcagatt cagccttttt aatctccaga
     8401 ggatcatcac ctgcagcaag ttgctctcgc attatccggg cagtacgtgc agcttcagca
     8461 atactgacct ctgggtaagt tcccaatcca gcattacgtc ttttttgtgt caccggactt
     8521 acataacgaa aaacccattt cccccgcccc tttactgaag aaggatgaag ggtcagtccg
     8581 gtaattcccc catggggcaa tggtttgtca tcaggtttga tatgtcttgc tttcgtatcc
     8641 gtcaatactg ccatacgcta attcccgtca ctctggtatg ccatccagta tgccattagt
     8701 ccctcgcttc actaagaata cattagataa catcggacaa caaaaagtgt aatacattgt
     8761 ttataaagaa attatataac acccctggat taagtcagat ttatttcagg cggtccccct
     8821 caccgccata tttaaagaag agcgcgtacg aaagtacggg cttttttttc gtatattgta
     8881 cacaccgggt gcccccgccc acgtctgtta gggcgagggg aaattgtgca catcaactgt
     8941 ctggttactc agtcaacaat caaaatgcgt aagatagtac agaacatata tgcgttatct
     9001 ggtcacttcc tgtaacttcc tcacttatta aaatgccccc aacggaaata catgtcacat
     9061 gccatcataa aatcgttatg ccagaggaat cgacaatgga aaaaatagta aattaagcac
     9121 ggtacatagc agagtaaaaa ataggcttca aaccaaatcc gccattccac aagcgttgcc
     9181 aataccgaca ccagcgtaat ccacatcatt tcaataaccc gcaatatgaa taaccgaagc
     9241 caaagcggct tggcaaaaaa ggtcgacaac gtattttcac tggagaaaaa cagatcgatt
     9301 tcctgcaaaa aaaacctgcc cagaaaagcg ggccgcttgg agaagcgcag aaaaaacaga
     9361 ttaatcgcca acactacgcc acaaagaatg agcaatgcaa ttatcgattt aaacgttaac
     9421 cactgcccct ctgtcacaaa agaatcactg ttacgcaacg ggctgatagt aagcaggtta
     9481 acaaccgttg agatgcacac cgaaagcagc acatagcgag tcagactttt atattttact
     9541 tttttcgcta ctgaaatagc gacctgcttg acataactgg tgcggatcat ccgaaacagc
     9601 agaaagatga ccaacggccc aagtaacgaa atcaggaaca gcgcatataa cgagggtaga
     9661 agctggtata gcccccaact cattgcactc aacatgatta acgacagaaa tcctgccacc
     9721 agaggttgtg taagaaataa ttctattttg tggttctgct ttttcttaaa ccacgaaaaa
     9781 tcgatattct tcgtgacgca tttataagcc cagtacgcct tgagatcaaa aataaaatta
     9841 tacagcacca gggccagaat cccactaact acaccaatat cctctgatgg aaaagtaaac
     9901 cctgcatttt tccaataaaa gcctatcaga ataagtccaa tagcataaat gtatatcaga
     9961 tttcgtttat cctgaaagcg aataaaagtt aaataatcag gaatcatttg aatacctttg
    10021 catcactaaa tgctttacga caatgcataa ctcgttgctg atccacgcca gcaaaatcat
    10081 ttaacatatt ccccaaatag ggcttattac ttatttcaag gaaagctacc ggggttggca
    10141 tattcagaat atgtatcgag agattcagtc catttcaccg tatgagttaa gtgtaatgag
    10201 agattttctt tgataattga cggagcgacc tcgctttttg ctgttacgtt catcagaacc
    10261 tgatgctctg gcgaagcgat atccagtccg gcaagataat cgcgcattgc ctgaacgccg
    10321 tcttccatca aacgcgtgtg ccaggcaccg ctcacaccga gtttaaccgg ttcgtatcca
    10381 gcggccatca gcagcgtggt aaattcattc aacgcagcct gcgttccccc aataacctgc
    10441 tggcgcggcg tgttatcaca gctaatatcc agcgcaatgc ctgattccgt aatcatcgtc
    10501 tgcagttgtt cgcggttgat gcctttaacc gcctgatctt acccagcaat agtggacacg
    10561 cggctaagtg agtaaactct cagtcagagg tgacttacat gacaaaaaca gtatcaacca
    10621 gtaaaaaacc ccgtaaacag cattcgcctg aatttcgcag tgaagccctg aagcttgctg
    10681 aacgcatcgg tgttactgcc gcagcccgtg aactcagcct gtatgaatca cagctctaca
    10741 actggcgcag taaaca
//
LOCUS       DAJTQY010000118        10693 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.118, whole genome shotgun sequence.
ACCESSION   DAJTQY010000118 DAJTQY010000000
VERSION     DAJTQY010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10693
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.118"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(79..1428)
                     /gene="lysC"
                     /locus_tag="OO349_003399"
     CDS             complement(79..1428)
                     /gene="lysC"
                     /locus_tag="OO349_003399"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HCR5868348.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLELGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     gene            1953..3602
                     /gene="pgi"
                     /locus_tag="OO349_003400"
     CDS             1953..3602
                     /gene="pgi"
                     /locus_tag="OO349_003400"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HCR5868349.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHISEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            4064..4306
                     /locus_tag="OO349_003401"
     CDS             4064..4306
                     /locus_tag="OO349_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5868350.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAESAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTITTTTTSTQ"
     gene            4420..5058
                     /locus_tag="OO349_003402"
     CDS             4420..5058
                     /locus_tag="OO349_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HCR5868351.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            5055..5792
                     /locus_tag="OO349_003403"
     CDS             5055..5792
                     /locus_tag="OO349_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HCR5868352.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            5792..7888
                     /locus_tag="OO349_003404"
     CDS             5792..7888
                     /locus_tag="OO349_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HCR5868353.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYIDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAHKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(7935..8213)
                     /locus_tag="OO349_003405"
     CDS             complement(7935..8213)
                     /locus_tag="OO349_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5868354.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            8214..8357
                     /locus_tag="OO349_003406"
     CDS             8214..8357
                     /locus_tag="OO349_003406"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5868355.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYP
                     AYG"
     gene            8483..8893
                     /gene="psiE"
                     /locus_tag="OO349_003407"
     CDS             8483..8893
                     /gene="psiE"
                     /locus_tag="OO349_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HCR5868356.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(8937..10412)
                     /gene="xylE"
                     /locus_tag="OO349_003408"
     CDS             complement(8937..10412)
                     /gene="xylE"
                     /locus_tag="OO349_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HCR5868357.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKISAVLFFISGVGS
                     AWPELGFTSINPDNTVPIYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGRRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQTESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQASGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
BASE COUNT         2717 a         2730 c         2768 g         2478 t
ORIGIN      
        1 gtccgttaaa ctcaggctac ctcatagtga caagaaaatc aatacggccc gaaatatagc
       61 ttccaggcca tacagtattt actcaaacaa attactatgc agtttttgca ccacctgctc
      121 ggcatcttcg ccgggcacca ggaagcacag gttatggctg gatgcgccgt aacaaatcat
      181 gcgaatgttg aacggttcca gtacgccgaa tacctctttg ccaacgccgc aggcttttga
      241 caggtcattg ccaatcaacg cgaccagcgc cagaccttct tccacctcca cccggcacag
      301 tgcggaaagc tccatcagca gagattgcgt cagcaacgta tcgccagtgg aggttgaacc
      361 ggtggtatca agggttaatg ccacgctcac ttctgacgtg gtgattaagt ctaccgaaat
      421 attatgccgc gcgaggatgc cgaaaacttc cgcgaggaag ccgcgagaat gcagcatatt
      481 caggctgtgc aaagtgagca gagtctgatt gcgacgaagc gccagcgcgc ggaacagcgg
      541 cggattttca gttttattgc acaccagcgt accacctgcg cgtgggtctt tgctggagcc
      601 gacaaagacc gggatatcgc tgcgtactgc gggtagcaac gttgccggat gcagtacttt
      661 tgcaccaaaa gttgccatct ctgccgcttc ggcaaacgcg atttcatcaa tgcgttttgc
      721 tgcggaaact acgcgtggat cggtggtgta gatgcccggg acgtcggtcc agatatcaac
      781 acgagatgcg tgtaaagcct ccgccagcaa ggctgccgta taatcgctgc ctccacggcc
      841 aagcgtcgtt gtacgacctt tattttcgct accgataaat ccctgggtga tcactaagcc
      901 ttcattgagg cgtgggagca gctgcagcgc ggccagttcc gccagcgcgg ctacatctgg
      961 ctctgcacga ccaaatcggt cgttggtacg catcacttta cgtacatcaa accactgtgc
     1021 ctgaacatcg cgttcgcgca ggatttcgac aaacagcagg gtcgacatca gctcgccgtg
     1081 gctgaccagc tcatctgtca gcgccggaga cgttgccagc gccgccgctt ctgccagaac
     1141 agtaatgttc tccagcagac gttcaatctc ttcacggata acgttcgggt aacgcagacg
     1201 ttccagaatg gcaaactgga tgttgcgaat agcgtcgagt ttttcgaatc gctcgccaag
     1261 ttccagccct tccgctaaag cgaccagcag attagtgata ccagcagaag ccgagaggac
     1321 tactaaacgc acgttggcat cagaaagcac aatatcagcg ctgcggttca tggcgtcaaa
     1381 atcagctacg ctggtaccgc caaatttgga gacaacaatt tcagacataa ctacctcgtg
     1441 tcaggggatc cattttcagc cttggcacaa gggaagagcg gaagacgggt gggcgcagag
     1501 cgatacttcg ctactatttt cacccagaag tgctccacca cttgcgaaac gcccgactgc
     1561 gaacgcttct ggtgacaacc caggggattc agcccctgta gccgatgatg aacgtggcca
     1621 gccgttcaat cacctcggcg atgcaccccc tcaggtgtta tcacaggact ggctcctcca
     1681 acaccgttac ttgggcaacg cgcctcttct ggcctgcgct agcgcaggta gtacatttat
     1741 aaataaaggg tgagcggggc ggttgtcaac gacggggtca tgcggatttt tcatccactc
     1801 ctggcggtca gtagttcagc taataaatgc ttcactgcgc taagggttta cactcaacat
     1861 tacactaacg gcactaaaac catcacattt ttctgtgact ggcgctacaa tcttccaaag
     1921 tcacaattct caaaatcaga agagtattgc taatgaaaaa catcaatcca acgcagaccg
     1981 ctgcctggca ggcactacag aaacacttcg atgaaatgaa agacgttacg atcgccgatc
     2041 tttttgctaa agacggcgat cgtttttcta agttctccgc aaccttcgac gatcagatgc
     2101 tggtggatta ctccaaaaac cgcatcactg aagagacgct ggcgaaatta caggatctgg
     2161 cgaaagagtg cgatctggcg ggcgcgatta agtcgatgtt ctctggcgag aagatcaacc
     2221 gcactgaaaa ccgcgccgtg ctgcacgtag cgctgcgtaa tcgtagcaat accccgattc
     2281 tggttgatgg caaagatgtt atgccagaag tcaacgcggt gctggagaag atgaaaacct
     2341 tctcggaagc gattatctcc ggtgagtgga agggttatac cggcaaagca atcactgacg
     2401 tagtgaacat cgggatcggc ggttctgacc tcggcccgta catggtgacc gaagcactgc
     2461 gtccgtacaa aaaccacctg aacatgcact ttgtttctaa cgtcgatggg actcacatct
     2521 cggaagtgct gaaaaaagta aacccggaaa ccacgctgtt cctggtagca tctaaaacct
     2581 tcaccactca ggaaaccatg accaacgccc atagcgcgcg tgactggttc ctgaaagcgg
     2641 caggtgatga aaaacacgtt gcgaaacatt ttgcggcgct ctcaaccaat gccaaagccg
     2701 ttggcgagtt tggtattgat actgccaaca tgttcgagtt ctgggactgg gtcggcggcc
     2761 gttactcttt gtggtcagcg attggcctgt cgattgttct ctccatcggc tttgataact
     2821 tcgttgaact gctttctggc gcacacgcaa tggacaagca tttctccacc acgcctgccg
     2881 agaaaaacct gcctgtcctg ctggcgctga ttggcatctg gtacaacaat ttctttggtg
     2941 cggaaactga agcgattctg ccgtatgacc agtatatgca ccgtttcgcg gcgtacttcc
     3001 agcagggcaa tatggagtcc aacggtaagt atgttgaccg taacggtaac gttgtggatt
     3061 accagactgg cccgattatc tggggtgaac caggcactaa cggtcagcac gcgttctacc
     3121 agctgatcca ccagggaacc aaaatggtac cgtgcgattt catcgccccg gctatcaccc
     3181 ataacccgct ctctgatcac caccagaaac tgctgtctaa cttcttcgcc cagaccgaag
     3241 cgctggcgtt tggtaaatcc cgcgaagtgg ttgagcagga atatcgcgat cagggtaaag
     3301 atccggcaac gcttgactac gtggtgccgt tcaaagtgtt cgaaggtaac cgcccgacca
     3361 actccatcct gctgcgcgaa atcactccgt tcagcctggg tgcgttgatt gcgctgtatg
     3421 agcacaaaat ctttactcag ggcgtgatcc tgaacatctt caccttcgac cagtggggcg
     3481 tggagctggg taaacaactg gcaaaccgta ttctgccaga gctgaaagat gataaagaaa
     3541 tcagcagcca cgatagctcg accaatggtc tgattaaccg ctataaagcg tggcgcggtt
     3601 aatcatcatg cgtgcctgat gccgatatgt aggccggata aggcgtttac gccgcatccg
     3661 gcaaccggag cctgatgcca atatcgacat taatgcatag tatgaggagt taaataacct
     3721 taaaaacaat gccgacaata agtcggcatt gttttatcag ataaatcccc ttgtctgtga
     3781 tttaacggaa atcataccgt gaggttaatc ctaaaataga tttttaatcg ttgtttattt
     3841 cggagaatac gcagattaat tacttttgtt tttattttaa gcttatgatt tttattgtta
     3901 tttaaatata agttgaaact tatatttgat attcattcca attatcctaa aactccatcg
     3961 ctaattcccc gcgccgtaat tcgcatgctt tagttgtgta tactcgatcc cgcccgaaat
     4021 gtttttgggt aaatctccat tcattcaatg aagggaaatt gttatgaaaa aagttctgta
     4081 tggcattttt gccatatctg cgcttgcggc gacttctgcg tgggctgcac ctgtacaggt
     4141 gggcgaagcg gcagagtcgg cagcaacgtc ggtttcggcg gggagttcct ccgcgaccag
     4201 cgtcagcacc gtaagctcgg cggtgggtgt cgcgctcgcg gcaaccggtg gcggtgatgg
     4261 ttctaatacc gggaccatca caaccacgac caccagtacc cagtaataaa gtatgtatcc
     4321 ccaaaataat ttgagtcatt gcatctgtgg cttgaagtat gaagggattt accataacca
     4381 cactccggtg tggttattct gcccctctgg agaagagtcg tgaagcgacc tgcactcatt
     4441 cttatctgcc tgctattaca ggcctgttca gccacgacta aagagctggg caattcactg
     4501 tgggacagtc tgttcggcac gccaggcgta cagctgacgg acgatgatat tcaaaatatg
     4561 ccctacgcca gccagtacat gcagcttaat ggcgggccgc aattgtttgt ggtgctggcc
     4621 ttcgctgaag acggacaaca aaaatgggtc actcaggatc aagccactct tgtcacacaa
     4681 catggtcgtc tggtgaagac tttgcttggc ggcgacaacc tgattgaagt gaataacctt
     4741 gccgccgacc cgctgattaa acccgcacaa attgttgatg gtgcaacatg gacgcgcacg
     4801 atgggctgga ccgagtacca gcaggtacgc tacgccaccg cacgctcagt cttcaaatgg
     4861 gatggcaccg ataccgtcaa ggtcggcagc gatgaaaccc tggttcgcgt gctggacgaa
     4921 gaagtctcca ccgaccaggc gcgctggcat aaccgctact ggatcgacag cgaagggcaa
     4981 attcgccagt cggaacagta tctcggcgcg gattatttcc cggtgaaaac cactctcatc
     5041 aaggcggcaa aacaatgatt aaacaaacta ttgtcgcgtt gcttttgagt gtgggagcgt
     5101 catcggtctt cgcggcagga accgtcaagg tgttcagcaa tggcagcagt gaggccaaaa
     5161 cgctaacggg cgcagagcat ttaatcgatc tggtagggca acctcgactg gcaaacagct
     5221 ggtggcctgg tgcggtgatt agcgaagagc tggcaacggc ggcagcattg cgtcagcagc
     5281 aggcgttgct gacacggctg gcagaacagg gcgcagattc cagcaccgac gatgccgccg
     5341 ccattaacgc cttacgccag caaatccagg cattgaaagt gacaggcagg caaaaaatca
     5401 atcttgatcc cgatatcgtc cgcgttgccg aacgcggtaa cccgccgttg cagggcaact
     5461 acacgctgtg ggtcggacca ccgccgtcca cggtcacgtt gttcgggctt atcagccatc
     5521 ctggcaatca gccattcact ccaggtcgcg acgtggcgag ctatctctct gaccagagcc
     5581 tgctcagcgg tgcggatcgc agctacgcgt gggtcgttta cccggacgga cgcacgcaaa
     5641 aagcgccggt ggcttactgg aacaagcgtc acgtagagcc gatgcccggc agcattattt
     5701 atgttggcct cgcagattcc gtctggagtg agacgcctga tgcccttaac gccgacattc
     5761 ttcagactct gacgcagcgg ataccccaat aatgaaaaaa agacatctgc ttagcttact
     5821 ggcgctgggc attagcacag cttgctacgg cgaaacatat cctgcgccca ttggtccgtc
     5881 gcagtcggat ttcggtggcg taggattatt acaaactccc accgcgcgta tggcacggga
     5941 aggggagttg agtctgaact atcgcgataa cgatcagtac cgttattact cagcttcagt
     6001 gcaactcttc ccgtggctgg aaacaacgct gcgctacacc gacgtgcgca cccggcagta
     6061 cagcagcgtc gaagcgttct ctggcgatca aacgtataaa gataaagcct tcgatctcaa
     6121 actgcgtttg tgggaagaga gttactggct gccgcaagtg gcggttggtg cgcgggatat
     6181 cggcggtacg gggctgtttg atgcggaata tcttgttgcc agcaaagcct gggggccgtt
     6241 cgattttacg ctcggcctgg gctggggata tctgggcacc agcggtaatg taaaaaatcc
     6301 gctctgttca gccagtgata aatattgcta tcgcgataac agctacaaac aggcgggatc
     6361 tatcgacggt agccagatgt tccacggtcc tgcctcactg tttggcggcg tggaatacca
     6421 gacgccctgg caaccgctgc gtctgaaact ggagtatgaa ggtaataatt atcagcagga
     6481 ttttgccggg aagctggagc aaaaaagtaa gtttaacgtc ggggcgattt atcgcgttac
     6541 cgattgggcc gacgttaacc ttagctatga acgtggcaac acctttatgt ttggcgtcac
     6601 gctgcgcacc aactttaacg atctgcgccc gtcctacatc gataacgccc gcccgcaata
     6661 tcaaccgcag ccgcaggatg ccattttgca gcattcggta gtggcgaatc agttaacgtt
     6721 gctgaaatac aacgccggac ttgccgatcc gcagatccag gcgaaaggcg atacgctgta
     6781 tgttaccggc gagcaggtga aatatcgtga ttcgcgcgaa gggatcatcc gtgctaatcg
     6841 gatcgtgatg aacgatctgc cggatgggat caaaacgatc cgcattacgg aaaatcgcct
     6901 taacatgccg caagtgacga cggaaaccga tgtcgccagc ctgaaaaatc atctcgccgg
     6961 agagccgttg ggccacgaaa cgacgctggc gcacaaacgc gtcgagccag tggttccgaa
     7021 gtccaccgag cagggctggt atatcgacaa atcacgcttt gatttccata tcgatccggt
     7081 gctgaaccag tcggtcggtg gcccggaaaa cttttacatg tatcagctgg gcgtgatggg
     7141 aacggcagat ttgtggctga cggaccatct actgaccacc ggcagcctgt ttgcaaatct
     7201 tgccaacaac tacgacaagt ttaactacac caatccaccg caggactcgc acttaccgcg
     7261 cgttcgtacc catgtgcgcg agtatgtgca gaacgatgtc tatgtgaata acctgcaagc
     7321 caactacttc cagcatctgg gcaacggctt ctacggtcag gtctatggag gttatctcga
     7381 aaccatgttt ggcggtgcgg gggccgaagt gttgtatcgt ccgctggaca gtaactgggc
     7441 gtttggtctg gatgccaact acgttaaaca gcgcgactgg cgtagtgcaa aagatatgat
     7501 gaaattcacc gactacagcg tgaaaaccgg acatctgacc gcctactgga cgccatcttt
     7561 cgcccaggac gtgttggtga aagccagcgt cgggcagtat ctggcagggg ataaaggcgg
     7621 cacgctggag atcgctaaac gctttgacag cggcgtggtg gtgggtggct atgccacgat
     7681 caccaacgtt tcgaaagagg agtacggcga aggggacttc accaaaggcg tgtatgtctc
     7741 ggtaccgttg gatctcttct cgtctggccc gacacgcagc cgtgcggcga ttggctggac
     7801 gccgctgacg cgtgacggtg gtcagcaact tgggcgtaag ttccagttgt atgacatgac
     7861 cagcgaccgt agcgtaaatt tccgctaagt catgggaaag gtgccagttt tcgcactcac
     7921 tggcaccctc attcttaatg acctgttgag ccatcaggaa cgcagttcac ccccggttta
     7981 cgatctggtg tgtaaaggcg aaaacattct gtcttcctgt ccgtcgatat cggttgcagg
     8041 tctggttgcc tgagcgactg ggccatcatt gggctggaga cattgctttg cggtaggtcg
     8101 gctttatcag cagtaccgag cggaccagca taagcaggaa gaacagagac tgataacatc
     8161 aaagcagcaa aataaggctt catttttacc acctttatca ggttacgttt catttgttcc
     8221 agaggaacat tgtcgatttt tcgcgcattg ctggtggctg ggaatcacct gaatgggtga
     8281 tttttgaatt accggctttg gtgcggtttg tcttgccgga tgcgccgcca ggcgcggctt
     8341 atccggccta cgggtaggta tatccggctt tggtggaggc gcgctccaaa tccaggttga
     8401 acaaaacata cacaaaaaat atagatctcc gtcacatttt tgcgttatac aggaagctcg
     8461 ccactgtgaa ggaggtactg ctatgacgtc actctctcgt ccgcgcgtgg agtttatctc
     8521 caccattttg cagaccgtac tcaatcttgg cctgctgtgc ctcggcctga ttttggttgt
     8581 cttcctcggc aaagaaacgg tgcatctggc tgatgtgctg ttcgcgccag aacaaaccag
     8641 caaatatgag ctggtagaag gactggtggt ttactttctc tatttcgaat ttatcgcgct
     8701 gattgtgaag tactttcagt ccggttttca cttcccgcta cgttactttg tctatattgg
     8761 gatcaccgcc attgtgcgct tgatcatcgt cgatcataaa tcgccactgg atgtgttgat
     8821 ctactcggct gcgatcctgt tactggtgat caccctgtgg ctgtgtaatt cgaaacggct
     8881 gaagcgggag taaaaagaca gcacgccgaa atggcgcggc gtgctggaca ggaagattac
     8941 agcgtagcag tttgttgtgt tttcttcgtt tccggttccc agagcgcttc cagctcctca
     9001 agggttttac ctttggtttc cgggacaaat ttccacataa acagtgctgc cagaacgccc
     9061 atacaaccgt aaatccagta ggagaaaccg ttgtggaaat gggccaccag ccaggagttt
     9121 ttgtccatca tcgggaaggt ccaggagacg aagtagttcg ccagccactg ggccgccacc
     9181 gcgattgcca gcgctttacc acgaatagca ttcgggaaga tttccgacag cagtacccag
     9241 cataccggac cccaggacat ggcaaaggcg gcaacataga acagcatcga cagtagcgcc
     9301 acaatacccg atgcctgagt gtaaaacgcg gtaccgaggc taaacatacc gattgccatt
     9361 ccgagtgcgc cgataatttg cagtggctta cgaccaaatt tatccacagt cataattgcc
     9421 agtacggtaa aggtgaggtt gataactccg acaataatgg tctgcaacag cgcgatatcc
     9481 gtgctggccc ccagcgtttt gaacacttcc ggcgcgtagt acagcaccac attgatgccg
     9541 acaaattgct ggaagatgga gagcataacg ccgattacaa tcacgcccac gccaaacatc
     9601 agcagacgac caccggtttt gcggccatga tccagggagt gtttaatttc ctgtactgcc
     9661 tgagttgcaa gcgtgttgcc cataattttg cgcaggatac tttccgtctg ttcttgcttg
     9721 ccgcgtgaca tcagccagcg aggactttct ggcacggtat acagcagcat taagaacagc
     9781 agtgcaggga tacattccga ggcaaacata taacgcctgc cgtcagtatt cagccagctg
     9841 gcatcaccgg aacgggcaat aaaatagttt acgcagtaaa ctaaaagttg cccgaaaata
     9901 atcgcaaact ggttaaatga aaccagtttt ccgcgaatat gagctggagc cagttccgca
     9961 atatacattg gtgaaagcat tgaggctaaa ccaacgccaa taccgccaat aatacgataa
    10021 ataacaaatt ccgggacata acctgccaga taaataggta ctgtgttgtc cgggtttata
    10081 gaggtaaaac caagttctgg ccaggcagaa cctacaccag aaataaaaaa caggacagca
    10141 gaaatcttaa gtgaatcacg acggccgaag cggttactgc aataaccacc gagggcaccg
    10201 ccgatgatgc aaccaatcag agcgctggct acgcaaaatc ctaacaggga gttggcagcg
    10261 gattcactta agttttgtgg agcaacaaag acggtattga gtgactcaac agtaccggaa
    10321 ataacggcgg tgtcgtagcc aaataataaa ccacctaatg tagcgactaa ggtaatcgaa
    10381 aatatataac tggaattata ctgggtattc attcagacct gccttagacc attctgatct
    10441 tttatcagtg cattgatgtt ggaattgtca ggcagttaag gcaattatca ttttttacaa
    10501 tgcgtttacg taatgtcttt tctgtgatct taattgtgat aattatcaaa aattgatgaa
    10561 aacaatacta ttgccgtgac tcagagcacg aaagagaatt atcgtaagtg ggaaaacaaa
    10621 taacgtaaaa ataataagtt ctatgatggg aatattaacc ggcgaacgat tcagattgca
    10681 gacgaaagaa aaa
//
LOCUS       DAJTQY010000119        10629 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.119, whole genome shotgun sequence.
ACCESSION   DAJTQY010000119 DAJTQY010000000
VERSION     DAJTQY010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10629
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.119"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            51..1541
                     /locus_tag="OO349_003409"
     CDS             51..1541
                     /locus_tag="OO349_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HCR5868358.1"
                     /translation="MDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAIEAAQKS
                     TPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGIPLTETF
                     LPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQNRGFDY
                     FMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPFMLYLAY
                     NAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQYDNTII
                     LFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKLISAMDF
                     YPTALDAADISIPKDLKLDGVPLLPWLQDKKQGEPHKNLTWITSYSHWFDEENIPFWD
                     NYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTDLQQKDN
                     LAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            1593..2750
                     /locus_tag="OO349_003410"
     CDS             1593..2750
                     /locus_tag="OO349_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCR5868359.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIEASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(2716..2838)
                     /locus_tag="OO349_003411"
     CDS             complement(2716..2838)
                     /locus_tag="OO349_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5868360.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            3041..4726
                     /locus_tag="OO349_003412"
                     /pseudo
     CDS             3041..4726
                     /locus_tag="OO349_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            4764..7124
                     /locus_tag="OO349_003413"
     CDS             4764..7124
                     /locus_tag="OO349_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HCR5868361.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMYFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRLDWLTSHIDENNKSAFNQGSSGSTYFSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQNLKTPYNDELAMGLQQKIDKNVIARAS
                     EAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHIRQVDINPQIVFS
                     YIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFTHQPS
                     GLVWANTLAWQETRKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSWTPQF
                     LKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            7181..9964
                     /locus_tag="OO349_003414"
     CDS             7181..9964
                     /locus_tag="OO349_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR5868362.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNDLRYMI
                     YPHAHPKDQVNLWLQIHTGSLQEEDNELGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYISYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFVEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGAKVILAKSAGEEQKLQIIAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQLMYETRYADDRTKLLQENQIAQFTA
                     ADALAADRQLFSSPADITFVIVGNVAEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDEQLTLANEVMVKRLTKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIINSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
     gene            10325..>10629
                     /locus_tag="OO349_003415"
     CDS             10325..>10629
                     /locus_tag="OO349_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR5868363.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCV"
BASE COUNT         3151 a         2343 c         2296 g         2839 t
ORIGIN      
        1 cgccgactga atacagtacc aaaggaaagc caaatattat cgtactaacc atggatgatc
       61 ttggttatgg acaacttcct tttgataagg gatcttttga cccaaaaaca atggaaaatc
      121 gtgaagttgt cgatacctac aaaataggga tagataaagc cattgaagct gcacaaaaat
      181 caacgccgac gctcctttca ttaatggatg aaggcgtacg ttttactaac ggctatgtgg
      241 cacacggtgt ttccggcccc tcccgtgccg caataatgac cggtcgagct cccgcccgct
      301 ttggtgtcta ttccaatacc gatgctcagg atggtattcc gctaacagaa actttcttgc
      361 ctgaattatt ccagaatcat ggttattaca ctgcagcagt aggtaaatgg cacttgtcaa
      421 aaatcagtaa tgtgccggta ccggaagata aacaaacgcg tgactatcat gacaacttca
      481 ccacattttc tgcggaagaa tggcaacctc aaaaccgtgg ctttgattac tttatgggat
      541 tccacgctgc aggaacggca tattacaact ccccttcact gttcaaaaat cgtgaacgtg
      601 tccccgcaaa aggttatatc agcgatcagt taaccgatga ggcaattggc gttgttgatc
      661 gtgccaaaac acttgaccag ccttttatgc tttacctggc ttataatgct ccgcacctac
      721 caaatgataa tcctgcaccg gatcaatatc agaagcaatt taataccggt agtcaaacag
      781 cagataacta ctacgcttcc gtttattctg ttgatcaggg tgtaaaacgc attctcgaac
      841 aactgaagaa aaacggacag tatgacaata caattattct ctttacctcc gataatggtg
      901 cggttatcga tggtcctctg ccgctgaacg gggcgcaaaa aggctataag agtcagacct
      961 atcctggcgg tactcacacc ccaatgttta tgtggtggaa aggaaaactt caacccggta
     1021 attatgacaa gctgatttcc gcaatggatt tctacccaac agctcttgat gcagccgata
     1081 tcagcattcc aaaagacctt aagctggatg gtgttccctt gctgccctgg ttgcaagata
     1141 agaaacaagg cgagccacat aaaaatctga cctggataac ctcttattct cactggtttg
     1201 atgaggaaaa tattccattc tgggataatt accacaaatt tgttcgccat cagtcagacg
     1261 attacccgca taaccccaac actgaggact taagccaatt ctcttatacg gtgagaaata
     1321 acgattattc gcttgtctat acagtagaaa acaatcagtt aggtctctac aaactgacgg
     1381 atctacagca aaaagataac cttgccgccg ccaatccgca ggtcgttaaa gagatgcaag
     1441 gcgtggtaag agagtttatc gacagcagcc agccaccgct tagcgaggta aatcaggaga
     1501 agtttaacaa tatcaagaaa gcactaagcg aagcgaaata actaaacctt catgcggcgg
     1561 atttttccgc cgccttattg agcgagatag cgatgcacgt tacagccaag ccctccagtt
     1621 ttcaatgtaa tctcaaatgt gattactgtt tttaccttga aaaagagtcg cagtttactc
     1681 atgaaaaatg gatggatgac agcactctga aagagttcat caaacaatat atcgaagcgt
     1741 ctggcaatca ggtctatttt acctggcaag gcggtgaacc cactctggct ggcctggatt
     1801 ttttccgtaa agttattcac tatcaacaac gctatgcagg ccaaaaacgt atttttaatg
     1861 cattacaaac gaatggcatt ttattgaata atgaatggtg tgcctttctc aaagaacatg
     1921 aatttctggt tggtatctcg atcgatggcc cccaggagtt acatgaccgt tacagacgca
     1981 gtaattcagg taacggtact tttgcaaaag tgatagcagc catcgagcgt ctgaaatcat
     2041 atcaagtaga gtttaatacg ttaaccgtca ttaataacgt taatgtccat tatcctcttg
     2101 aggtttatca ttttttaaaa tctatcggca gtaaacatat gcaatttatc gaattgctag
     2161 aaaccgggac gccgaatatt gatttcagtg gtcatagtga gaacacattc cgtatcattg
     2221 atttttctgt gcctcccacg gcttatggca agtttatgtc aaccattttt atgcaatggg
     2281 ttaaaaacga tgtgggtgaa attttcattc gtcagtttga aagctttgtc agccgttttt
     2341 tggggaatgg gcatacaagt tgtattttcc aggagtcctg caaggataat ctggttgttg
     2401 aaagtaatgg agacatttac gaatgcgacc attttgtcta tccacagtac aaaattggaa
     2461 acattaataa atctgaactc aaaacgatga acagtgtaca actgacagcg caaaaaaaac
     2521 ggatttcagc gaaatgtcag caatgtgcat ataaacctat ctgcaatggc ggttgtccta
     2581 agcatcgtat tactaaagta aacaatgaga ctgtttctta tttttgcgaa ggttataaaa
     2641 tccttttttc aaccatggta ccttatatga acgccatggt tgagttagct aagaacagag
     2701 taccgcttta ccacattatg gatgttgcaa aacaaatgga gaataattaa taacgctctc
     2761 ttttaactcc attcttagcc tccttcttat gaccttaaca aggcacccga cataaattcc
     2821 tgtcgggtgt ttcgtcatca tcatcttccc ttgcgaaaat cctggcttac gtggtatgcg
     2881 tcagggcatt agcaataccc ttgattatca atagcataac cataaccact gaaaattaaa
     2941 aatttctata tatttcatat tgttaaatga tgcttaaata aatttcatca gtaaatcatt
     3001 gccatagata atcattggat ggcacaatca attttcgcta atgataacca ttcccattac
     3061 gttttgtatg ttgatagcaa agtatctctg tctgctgaag cccttttggc tgcgtaagaa
     3121 taataaaact tcagtgttgt taatcattat tatcctcgca atgatccttg gcgtggttaa
     3181 aattcaggtc tggttaaacg actggaataa tgattttttc aatgcgctaa gccagaaaga
     3241 aaccgataag ctttggcagc tcgttctttg gttccctgcc ctgctgggga tctttgtatt
     3301 aatatccgta aacaaaacat ggttaattaa actactgact attcgctggc gcgaatggtt
     3361 aacagattac taccttaatc gctggtttgc agataaaaac tactacttca cgcagatcta
     3421 cggcgagcat aaaaatacag ataaccctga ccaacgtatc gctgaagaca ttctcttact
     3481 gatcaacaaa acgctcagtc tttcatttgg cttcatccag tcacttagca tgctgatcac
     3541 ctttaccgtt attctttggc aaagcgcggg tacgctctca ttcactgttg gtggaacaga
     3601 atggaatatc caggggtata tggtctatac cgtcgtgctc attgtgatcg gtggaacttt
     3661 atttacccat aaggtgggta aacgtattcg tccgcttaat gtggaaaaac aacgtagcga
     3721 agcgacattt cgaactaatc ttgtgcagca taataagcag gcagaactga ttgcgctaag
     3781 caatgctgag tctctacaac gccaggagtt gcgcgacaat tttcatacca ttaaagagaa
     3841 ctggcatcgt ttaatgaatc gtcagcggtg gcttgattac tggcaaaata tttattcgcg
     3901 ctcgcttagc gttcttccct actttctgtt attgccgcag tttattagcg ggcagatcaa
     3961 tctgggcgga ctgatgaaat cgcgccaggc atttatgcta gtatcgaaca atttaagctg
     4021 gtttatttat aaatatgacg aacttgctga actggctgcg gttatcgatc gcttgtatga
     4081 gttccatcaa ctcactgaat agcgccctac gaataagcct aaaaattgcc aacatgcggt
     4141 acaagtggct aatgcgagta ttcgtacgcc tgataataag atcatattag agaacttgaa
     4201 ctttcatgtt tcgccaggca aatggctatt actgaaaggc tactctggtg cgggaaaaac
     4261 cacactgctt aaaacattat cccactgctg gccgtggttt aaaggtgata tttcttctcc
     4321 tgctgacagt tggtatgtgt cacaaacacc gttaatcaaa agcggcttac tgaaagagat
     4381 tatttgtaaa gcacttcccc tgcccgtaga cgataaatcg ttgagcgaag tactgcatca
     4441 ggttggtctg ggaaaattgg ctgcgcgtat tcatgaccac gatcgctggg gagatattct
     4501 ttccagcggc gaaaaacaac gtatcgccct ggcacgatta attttacgac gtccgaaatg
     4561 gatatttctt gacgaaacta cctctcatct tgaggaacaa gaggctatac gcttactgcg
     4621 tttagtgcgt gaaaaactac ccacaagcgg cgtcattatg gttacacatc aacccggtgt
     4681 ctggaacctg gctgatgata tttgtgacat tagcacggtt ttataacaac aataaaagcc
     4741 ctgctgtacg gagatataaa taaatgaagc gagttcttat tcctggcgtc attttatgtg
     4801 gcgctgatgt ggcgcaggcc gtcgatgaca aaaacatgta catgtatttt tttgaagaga
     4861 tgacggtcta tgctcctgtc cctgtacccg taaacggcaa cacgcattac accagtgaaa
     4921 gcatcgagcg tttaccgacc gggaatggca atatcagcga tctgctgaga accaaccctg
     4981 cggtacgcat ggattcaacg caaagtacct cgttgaacca gggagatatt cgccctgaga
     5041 aaatctctat tcacggtgcg tcgccctacc agaatgccta tttgattgac ggtattagtg
     5101 caactaataa cctgaaccca gcgaatgagt ccgatgccag tagtgcaacc aatattagcg
     5161 ggatgtcaca ggggtattat cttgatgtca gcttactgga caatgtgacg ctttatgaca
     5221 gttttgtgcc ggttgaattt ggtcgcttca atggcggggt aattgatgca aagatcaaac
     5281 gcttcaacgc tgatgatagc aaggtgaaat tgggttatcg cactacgcgt ttggactggt
     5341 taacatcgca tatcgatgag aataacaaga gcgcatttaa tcaaggttct tcaggaagta
     5401 cctatttctc tccagatttt aaaaagaact tttatacctt gtcgtttaat caggagctgg
     5461 ctgataactt tggcgttacc gccggtttat cgcgccgcca gtctgatatc acccgcgcgg
     5521 attatgtttc gaatgacggc attgtcgccg gtcgggcaca gtataaaaac gttatcgata
     5581 ctgcattgag caaatttacc tggtttgcca gcgaccgctt tacccacgat ttaaccttaa
     5641 aatataccgg ctccagccgt gattataata ccagcacctt cccgcagtct gatcgcgaaa
     5701 tgggtaataa atcctatggt ctggcatggg atatggatac gcaactcgca tgggccaaac
     5761 tacgtaccac cgttggttgg gatcatatta gtgattatac ccgtcacgat catgacatct
     5821 ggtacaccga actttcatgt acatatggtg atattacagg gcgttgtacc cgtggcggat
     5881 taggacacat ttcccaggct gtagataatt acaccttcaa aacacgcctg gactggcaaa
     5941 aattcgccgt gggtgatgtt tcgcatcaac cctacttcgg cgcggaatac atctattccg
     6001 atgcatggac tgaacgccat aaccagtctg aatcctatgt gattaatgct gcaggaaaga
     6061 aaactaacca taccatttac cataaaggta aaggcagcct gggaattgac aactacacgc
     6121 tgtatatggc ggatcacatt agctggcgga atgtgtcgtt aatgcccggt gtgcgttatg
     6181 actatgacaa ctatctgtca aaccacaata tctccccgcg ctttatgacg gaatgggata
     6241 tttttgctga tcaaacctca atgattaccg ccggttataa ccgttactat ggcgggaata
     6301 ttcttgatat gggattacgt gatatccgca atagctggac ggaatcggta tcaggtaata
     6361 aaaccctgac gcgttatcag aatttgaaaa cgccttataa cgatgaactg gcaatgggat
     6421 tgcagcaaaa aatcgataag aacgttattg cacgcgcaag tgaagcgcat gatcaaatca
     6481 gcaaaagcag tcgtaccgac agcgcgacta aaaccaccat tactgaatat aacaacgatg
     6541 gcaaaaccaa aacgcattcg tttaacctca gttttgaact ggccgaaccc ctgcatatcc
     6601 gccaggtaga tattaacccg caaattgtct ttagctatat caagagcaag ggcaacttgt
     6661 cgttaaacaa tggttatgag gagagcaata ccggtgataa ccaggtggtt tataacggta
     6721 atctggtctc ttacgatagc gttccagtgg cagattttaa taacccatta aagatctcct
     6781 taaacatgga tttcacgcat caaccgagcg ggttagtgtg ggcgaatacg ctggcctggc
     6841 aagaaacgcg taaagctcgc attatccttg gtaagacaaa tgcgcaatac atcagcgaat
     6901 attcagatta caagcagtat gttgacgaaa aactggatag cagcctgacc tgggacaccc
     6961 gcttgtcctg gacgccacaa tttctgaaac aacaaaacct gacgatcagt gccgatattc
     7021 tcaatgtact ggatagcaaa accgctgttg atacaacgaa caccggtgtg gcgacctacg
     7081 ccagtggccg tactttctgg cttgatgtca gcatgaaatt ttaatagttc ggattttact
     7141 taaccgggta aattcgcccg gttttcgcat ggagataatt atgagaaacc tctgtttctt
     7201 actgacgtta gtggcaactc tgttgctccc cgggcggctg attgccgccg ccttaccgca
     7261 ggatgaaaag ttaattaccg ggcaactgga caatgacttg cgatatatga tttatccgca
     7321 tgctcatcca aaggatcagg taaatttatg gctgcaaatt cataccggtt cattgcagga
     7381 agaagacaat gagctcggcg tggctcattt tgtagaacat atgatgttta acggcacaaa
     7441 aacatggccg ggtaataaag tcatcgaaac atttgagtca atgggcctgc gttttggtcg
     7501 cgatgttaat gcctatatca gctatgacga aacggtgtat caggtgagtt tgccgactac
     7561 gcagaaacaa aatctgcaac aagtgatggc aatcttcagt gaatggagta atgccgcaac
     7621 ctttgaaaaa ctcgaagtag acgctgaacg tggcgtaatt actgaggaat ggcgtgccca
     7681 tcaggatgcg aaatggcgca cctctcaggc gcgccgccct ttcctgctgg ctaatacccg
     7741 taatttagac cgtgaaccta tcggcctgat ggatactgtc gccacggtca cgccggcaca
     7801 attgcgccaa ttttatcaac gctggtatca accaaataat atgaccttta tcgtggtcgg
     7861 cgatatcgac agtaaagaag cgctggcgct gataaaggat aatttaagta agcttccggc
     7921 taacaaagca gctgaaaatc gcgtctggcc gacaaaagcc gaaaaccacc tgcgctttaa
     7981 tatcattaat gataaagaaa accgggtgaa cggcatcgca ctctattatc gcctgccaat
     8041 ggtacaagtg aacgatgagc aaagctttgt cgaacaagct gaatggagca tgttagttca
     8101 gctgttcaat caacgtctgc aggaacgcat acagtcgggc gagttgaaga ctatttctgg
     8161 cggcactgcg cgcagcgtta aaattgcacc cgattatcag tcgctgtttt tccgtgtaaa
     8221 tgcacgagac gataatatgc aggatgctgc gaatgcatta atggcagagt tggcaaccat
     8281 tgatcagcat ggtttttctg ctgaagaact cgatgatgtc aaatctaccc gcctcacctg
     8341 gctgaaaaat gcggttgatc agcaagctga acgtgattta cgtatgctga ccagtcgcct
     8401 ggcatccagc tcattaaata atacgccgtt cttgtcgccg gaagagacat atcaactttc
     8461 gaaacgtctg tggcagcaaa ttaccgtgca aagtctggcg gaaaaatggc agcagttaag
     8521 aaagaaccag gacgcatttt gggagcaaat ggtaaacaat gagcttgccg ccaaaaaagc
     8581 attgtctcct gcggctatcc tggcgctgga aaaagagtac gccaacaaaa agctggcggc
     8641 ttacatcttc ccaggcagaa atttatcgtt aacagtagac gctgacccac aggcggaaat
     8701 tagcagcaaa gaaacgctgg ctgagaatct gacatcatta acactttcca atggtgccaa
     8761 ggttattctg gcaaaatccg cgggtgaaga gcaaaagcta caaattattg ccgtatcgaa
     8821 taaaggcgat ttaagtttcc ctgcgcagca aaaatcactt atcgcgctgg caaataaagc
     8881 agttagcgga agcggcgttg gcgaactctc ctcttccagc ctgaaacgct ggagtgcgga
     8941 aaactcggta accatgagca gtaaagtcag tggcatgaat acgttgctct ccgttagcgc
     9001 gcggactaat aaccctgaac ctggtttcca gttgattaac cagcgaatca cccacagcac
     9061 gattaacgac aatatttggg catcgctaca aaatgctcaa attcaggcgt tgaaaacgct
     9121 cgaccagcgt ccagcggaga aattcgccca gctgatgtat gagacgcgct atgctgatga
     9181 ccgcacgaaa ttactgcaag aaaatcagat tgcacagttt actgccgcag atgcgctggc
     9241 tgccgatcgc cagttgtttt catctccagc ggatatcacg tttgtcattg tcggtaatgt
     9301 cgcagaagac aaactcgtgg cgttaattac gcgttactta ggctcaatca aacactctga
     9361 ttcgccatta gccgcaggta aaccattaac tcgcgcgacg gacaacgcat cggttactgt
     9421 aaaagaacaa aatgaacctg tggcacaggt ttcacagtgg aagcgttatg attcccggac
     9481 acctgttaat ctggcgacgc gtatggcgct cgatgctttt aacgtcgcac tggcaaaaga
     9541 tctacgcgtt aatattcgtg aacaggcatc tggggcatac agcgtttctt ctcgcctctc
     9601 ggttgatcct caggctaaag atatcagtca tttgctggct tttacttgtc aaccagaacg
     9661 acatgatgaa cagttaacgt tagcgaatga agtgatggtt aagcgtttga ctaaggggat
     9721 cagtgagcaa gaactgaacg aataccagca aaacgttcag cgcagcctcg atatccaaca
     9781 gcgtagcgtt caacaattag cgaacactat tataaatagt cttattcaat atgacgatcc
     9841 tgcagcatgg actgagcagg agcaattgtt gaaacaaatg acggtagaga atgttaacac
     9901 tgccgttaaa caatatcttt ctcatccggt aaatacttat accggagtat tattgccaaa
     9961 ataataacag ccccgtcaac acatcgttgg cggggatttt agcaatattc gctattttta
    10021 tgtaataatt ttataaatgc gttcaaaata ataaccaagt actaatagtg atattttaat
    10081 gcctgatttt tacgtgataa ttcaggaggc acagaaagcg cataaaaata acaacataaa
    10141 acacctttcc accacccaag aattctctat tacattgttt ttaatgaaaa aatatcattc
    10201 caacaatatc tcacgataaa taacattagg attttgttat ttaaacacga gtcctttgca
    10261 cttgcttact ttatcgataa atcctacttt tttaatgcga tccaatcatt ttaaggagtt
    10321 taaaatggat aagaagcaag taacggattt aaggtcggaa ctactcgatt cacgttttgg
    10381 tgcgaagtct atttccacta tcgcagaatc aaaacgtttt ccgctgcacg aaatgcgcga
    10441 cgatgtcgca tttcagatta tcaatgatga attatatctt gatggcaacg ctcgtcagaa
    10501 cctggccact ttctgccaga cctgggacga cgaaaacgtc cacaagttga tggatttatc
    10561 cattaacaaa aactggatcg acaaagaaga atatccgcaa tccgcagcca tcgacctgcg
    10621 ttgcgtaaa
//
LOCUS       DAJTQY010000120        10540 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.120, whole genome shotgun sequence.
ACCESSION   DAJTQY010000120 DAJTQY010000000
VERSION     DAJTQY010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10540
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.120"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            556..687
                     /locus_tag="OO349_003416"
     CDS             556..687
                     /locus_tag="OO349_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001344347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA invertase"
                     /protein_id="HCR5868364.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNAFICAGSELIVARMGSWPEVVMA"
     gene            947..1942
                     /gene="pgl"
                     /locus_tag="OO349_003417"
     CDS             947..1942
                     /gene="pgl"
                     /locus_tag="OO349_003417"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HCR5868365.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESVLPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDYSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(1983..2936)
                     /locus_tag="OO349_003418"
                     /pseudo
     CDS             complement(1983..2936)
                     /locus_tag="OO349_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473949.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            3120..4172
                     /locus_tag="OO349_003419"
     CDS             3120..4172
                     /locus_tag="OO349_003419"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HCR5868366.1"
                     /translation="MKKIPCVMMRGGTSRAAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            4248..5681
                     /locus_tag="OO349_003420"
     CDS             4248..5681
                     /locus_tag="OO349_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HCR5868367.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKLIAYLLIGKIGNTMLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AMALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            5864..8044
                     /locus_tag="OO349_003421"
     CDS             5864..8044
                     /locus_tag="OO349_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HCR5868368.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMASGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAGNVSELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQR
                     VIGGLANIAEEYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTT
                     FKGYVIHEDAPVTEITLYMESLTAEEREIIKAGSLINFNKNRQM"
     gene            complement(8185..9468)
                     /locus_tag="OO349_003422"
     CDS             complement(8185..9468)
                     /locus_tag="OO349_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HCR5868369.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEALNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(9603..10484)
                     /locus_tag="OO349_003423"
     CDS             complement(9603..10484)
                     /locus_tag="OO349_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5868370.1"
                     /translation="MTARINSDNSVTLHSWLDRYEKILASRGIKQKTLINYMSKIKAI
                     RRGLPDAPLEDITTKEIAAMLNGYIDEGKAASAKLIRSTLSDAFREAIAEGHITTNPV
                     AATRAAKSEVRRSRLTADEYLKIYQAAESSPCWLRLAMELAVVTGQRVGDLCEMKWSD
                     IVDGYLYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCKEILGGETIIASTRREPLS
                     SGTVSRYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQHLLGHKSDTMA
                     SQYRDDRGREWDKIEIK"
BASE COUNT         2590 a         2420 c         2628 g         2902 t
ORIGIN      
        1 gtaattgtgc tgatatgtgg aaataaacag gatgaaatgt ttctgagcat attaatttct
       61 cgcatatgga tatggaatta gattgtgaat aaattgtatt taatgtatct ctcctaatga
      121 ggagggtgtt tcagaatatt cgccataagg gaatgagtgt tatatcatgc tttctaccct
      181 ggtaagtata tttgcttttt tcgtatattt ggttcatctt tttttgatgt tttacatatt
      241 cacaatgcag aggatttata ccagaggaca ttagtcttga gtaagatact acaacatctc
      301 caactatttt agccagtctg actacttgac ttggtaatat ttgttgctaa taatcatgta
      361 taaaataagc ttatatacat agtaacttca gcaaaggatt tagtaatgat caaaatcata
      421 tctcaatgta ctgttttttt ctttaaaaaa acagtacatt ctattttata atgactgtgt
      481 attcccatgg aaggagatca cccaattctc ccccccgact tttgacgcag tgtcttgtta
      541 acagggggat tgaccatgct tattggctat gtacgcgtat caacaaatga ccagaatacc
      601 gatctacaac gtaatgcgtt tatttgtgcc ggaagtgagt tgattgttgc gcgaatggga
      661 agttggccag aagttgttat ggcatagtgt ttacggtcgt tgatacaggt aaacaaaagc
      721 ggggagagtc atactccctg aatagtcgcg aaaatatctg tggcattgtc aaattcgtgt
      781 cggtgactaa tattttagta atatgccatt cgaaatggag tgctaactta ttgatttaat
      841 tgatgtgcga tattgtattt tatcgtgttt tgttgtctgt tttttatcca gtaagtgttg
      901 ttatatgtat gaaagataag ggatttatca tttttgaggt gtgttcatga agcaaacagt
      961 ttatatcgcc agccctgaga gccagcaaat tcacgtctgg aacctgaatc atgaaggcgc
     1021 actgacgctg acacaggttg tcgatgtgcc ggggcaggtg cagccgatgg tggtcagccc
     1081 ggacaaacgt tatctctatg ttggtgttcg ccctgagttt cgcgtcctgg cgtatcgtat
     1141 tgccccggac gatggcgcac tgacctttgc cgcagagtct gtgctgccgg gtagtccgac
     1201 gcatatttcc accgatcacc aggggcagtt tgtctttgta ggttcttaca atgcgggtaa
     1261 cgtgagcgta acgcgtctgg aagatggcct gccagtgggc gtcgtcgatg tagtcgaggg
     1321 gctggacggt tgccattccg ccaatatctc accggacaac cgtacgctgt gggttccggc
     1381 attaaagcag gatcgcattt gcctgtttac ggtcagcgat gatggtcatc tggtggcgca
     1441 ggaccctgcg gaagtgacta ccgttgaagg ggccggcccg cgtcatatgg tattccatcc
     1501 aaacgaacaa tatgcgtatt gcgtcaatga gttaaacagc tcagtggatg tctgggaact
     1561 gaaagatccg cacggcaata tcgaatgtgt ccagacgctg gatatgatgc cggaaaactt
     1621 ctccgacacc cgttgggcgg ctgatattca tatcaccccg gatggtcgcc atttatacgc
     1681 ctgcgaccgt accgccagtc tgattaccgt tttcagcgtt tcggaagatg gcagcgtgtt
     1741 gagtaaagaa ggcttccagc caacggaaac ccagccgcgc ggcttcaatg ttgattacag
     1801 cggcaagtat ctgattgccg ctgggcaaaa atctcaccac atctcggtat acgaaattgt
     1861 tggcgagcag gggctactgc atgaaaaagg ccgctatgcg gtcgggcagg gaccaatgtg
     1921 ggtggtggtt aacgcacact aaccgctgat ttaaccggcg cagtctctcc tgcgccggtg
     1981 tattaactta tctcctgtaa cgcatgtctc tggcgttcga cgatattggt ccacaaattg
     2041 tctttatcgt cagtccacaa attaatcagc aaggaaaaaa agcgttcagc tgccggagaa
     2101 agcacggcat ctttgcggcg aataatcccc aatgtccgac gtataacggg ttccaccagt
     2161 gggataccaa taatcgaaga atagggcgca tggggcattg ccagaccagg aagcgccgaa
     2221 atccccagtc ctgcctccac tagtcctaat gacgtcgaaa gatgacgcac ttcgtaaaac
     2281 cagtccagct tccagggctt gtcggctagc tgttgttcta tcaacaatcg gttgccgctg
     2341 gaggaacgta cgccaatcat tttgtaaccc actaactcct gccattctac aagctacttt
     2401 ttggccaatg gatggtcacg ccgacaggcc agcacgaatg gctcgttgac cagtggggta
     2461 aaatcaatgg atgaatttgt gacgttgttc atgtttatgc caaagtctga ttcgttacac
     2521 agcaccgatt ccatgcaatt attcgtgcct tgttccagaa tccgcacttt aatattggga
     2581 tacagctcat taaatttacc gattgccagc ggtaaaaaat aaaatactgc ggtcggaata
     2641 cacgctaatg tcaccatatc acgatgataa gcattcatat cacgaatatt aaaaagcgtt
     2701 tcatcgaatt tctttattaa ttcccgcgcc tccgggagca acctttttcc tgcttttgtt
     2761 aacgtaacct tgcgagttgt acgctcaaat agttgaatat gtaaatcttc ctccatcttt
     2821 tttattcttc gcgttaatgc aggttgcgta atattgagta atttagcggc attgttaaat
     2881 gaactggact ctgccagtat gacaaatgcc ttcatacttg ataattcatg cttcattatt
     2941 actccggaaa atggaagcga cgattttggg gggctggtcg ttaaaaattt taactgcatt
     3001 tagccaactt aaattaatga aaaaatgtta ttaatcgttg agctaaagtc attagagatg
     3061 ctttgtcctt aatgtaacca tatcgcaata agttatgttt ttaaattgag ggcattatta
     3121 tgaaaaaaat accctgcgtg atgatgcgag gtggaacctc gagggccgcg ttcctgttag
     3181 cggaacattt acccgaagat caaacgcagc gcgataaaat attgatggca attatgggtt
     3241 ccggtaacga tctggaaatt gacggtattg gcggcggtaa tccactgacc agtaaagtcg
     3301 ccattattag ccgttccagc gatccgcgtg ctgatgtcga ttatctgttt gctcaagtta
     3361 tcgtccatga gcaacgtgtc gataccacgc ctaactgcgg caatatgctg tctggcgtag
     3421 gggcattcgc cattgaaaat ggtttgattg cagcgacttc gccagttacc cgtgtacgta
     3481 tccgcaacgt caatacgggt acgttcatcg aagctgatgt gcaaacgcca aatggtgttg
     3541 tcgagtacga gggtagcgcc agaattgacg gcgtaccggg tactgccgca ccggttgcgc
     3601 tcactttcct gaatgccgct ggaacgaaaa ccggaaaagt tttcccgact gataatcaga
     3661 ttgattattt tgacgatgtc ccggtgacct gtatcgatat ggcgatgcca gtcgtcatta
     3721 ttccggctga atatctggga aaaacaggtt atgaattacc ggcggaactg gatgccgaca
     3781 aagcattatt agcccgcatt gaatctatcc ggctacaagc gggtaaagca atgggcttag
     3841 gtgatgtcag taatatggtt atccctaaac ctgtgcttat ttctccagcg cagaaaggcg
     3901 gggcaattaa tgtgcgttat tttatgccgc attcttgcca tcgcgcgctg gcgataaccg
     3961 gtgctattgc tatttccagt agttgtgcat tggaaggcac cgtcacccga caaatcgtcc
     4021 cttctgtagg atatggcaat atcaatattg aacaccccag tggtgcgctc gacgttcatt
     4081 taagtaatga aggtcaggat gccacgacgt tacgcgcatc tgttattcgg acaaccagaa
     4141 aaatattttc cggtgaagtt tatcttccct gaaaaaattc gttgtcagga taaggacaat
     4201 caataaagga cttctgtatg agtcatacag aaagaacagg attttaaatg aataagaaat
     4261 cgttatggaa gctaattctg atattagcga tcccatgtat tattggtttt atgccagctc
     4321 cggcaggatt aagcgaactg gcgtgggtgc tttttggtat ttacctggcg gccattgtgg
     4381 gtctggttat caagcctttc ccggaacctg tcgtactgtt aattgccgtt gctgcctcaa
     4441 tggtggtggt cggtaactta tccgacggtg cgtttaagac caccgccgta ttaagcggtt
     4501 actcttcagg tactacctgg ctggtgttct cggcgtttac cttaagcgcc gcatttgtga
     4561 ccaccggttt aggtaaactt attgcctatc tgctgattgg taaaatcggt aacaccatgc
     4621 tgggtctggg ttacgttacg gtattcctcg atctggtact ggctccggca acaccgtcta
     4681 acaccgcgcg tgcgggcggc attgtgttac cgatcatcaa cagcgtggcg atggctttgg
     4741 ggtccgaacc ggaaaaaagt ccgcgtcgtg tcggacatta cctgatgatg tccatttaca
     4801 tggtcaccaa aaccaccagc tatatgttct ttaccgcaat ggcggggaac attctggcgc
     4861 tgaaaatgat caacgacatt ctgcacctgc aaattagctg gggtggatgg gcgctggccg
     4921 ccggattgcc gggcatcatt atgctgctgg tcaccccgct ggtaatttac accatgtatc
     4981 caccagaaat taaaaaggtg gataacaaaa ccatcgctaa agcgggcctt gccgaactgg
     5041 gaccgatgaa aatccgcgaa aaaatgctgc tcggtgtctt tgtgctggcg ctactgggct
     5101 ggattttcag taagtctctg ggggttgatg aatccactgt ggcaatcgtt gttatggcga
     5161 ccatgctgct gctgggtatc gttacctggg aagacgtggt taaaaataaa ggcggctgga
     5221 ataccttaat ctggtacggc ggtattatcg gcttaagctc cttattatcg aaagttaaat
     5281 ttttcgaatg gttagctgaa gtctttaaaa ataacctggc atttgatggt cacggtaacg
     5341 ttgctttctt cgttattatt ttcctcagca tcatcgtgcg ttatttcttc gcttccggta
     5401 gtgcctatat cgttgctatg ttacctgtat ttgccatgct ggcgaacgtc tccggcgcac
     5461 cgttaatgtt aaccgcgctg gcactgttgt tctccaactc ctatggcggc atggttactc
     5521 actatggcgg cgcggcaggt ccggtcatct ttggcgtggg ttacaacgat attaaatcct
     5581 ggtggttggt cggtgcggta ctgacgatat taaccttctt ggtgcatatc accctcggcg
     5641 tgtggtggtg gaatatgctg atcggctgga acatgctgta aatatactcg tcatacttca
     5701 agttgcatgt gctgcgtctg cgttcgctca ccccagtcac ttacttatgt aagctcctgg
     5761 ggattcactc acttgtcgcc ttcctgcaac tcgaattatt tagagtatat ccatttatta
     5821 tctttctgcg cacttcacgg tgcgcagata tctggagcat ttgatgatca agttatctga
     5881 aaaaggcgtg tttctcgcca gtaataacga aataattgcc gaagaacatt tcaccggcga
     5941 aattaaaaaa gaagaagcaa aaaaaggcac tattgcctgg tctattctct cttctcataa
     6001 tacgtccgga aatatggata aacttaaaat taagtttgat tcattagcct ctcacgatat
     6061 tacctttgtt ggtattgtac agaccgctaa agcgtccgga atggaacgtt tcccgctgcc
     6121 gtatgtgctg accaactgcc ataactcact ctgcgccgtc ggcggcacca ttaacggtga
     6181 tgaccatgtt tttggtttat cggcagctca gcgttatggc ggtatttttg tgcctccgca
     6241 tattgcggtc atccatcaat atatgcgtga gatgatggct agcgggggca aaatgatcct
     6301 cgggtcagac agccacaccc gttacggtgc attagggaca atggcagtcg gtgagggtgg
     6361 cggtgagttg gtaaaacagc tgcttaatga cacctgggat atcgactatc cgggcgtggt
     6421 tgcggtgcat ctgaccggaa aacctgcgcc gtatgtgggg ccacaggatg tggcgctggc
     6481 tatcattggc gcggtgttca aaaacggtta cgtcaaaaac aaagtcatgg agttcgttgg
     6541 accgggcgtt agcgcgctct ctaccgattt ccgtaacagc gttgacgtga tgaccacgga
     6601 aaccacctgt ttaagctccg tctggcaaac tgatgaagaa gtgcataact ggctggcgct
     6661 gcacggtcgc ggccaggatt actgccagct taaccctcaa ccgatggcgt actacgatgg
     6721 ctgcatcagc gttgatttaa gcgccatcaa accaatgatt gcgctgccgt tccacccgag
     6781 caacgtgtat gaaatcgaca cactgaacca gaacctgacc gacattctgc gtgagattga
     6841 aattgagtcc gaacgcgtgg cgcacggtaa agccaaactc tcgctgctgg ataaagtgga
     6901 aaatggtcgc ctgaaagtgc agcaggggat tatcgcgggc tgttctggcg gtaactacga
     6961 aaacgtcatc gcggcggcga atgcactgcg cggtcaatcc tgtggcaatg acaccttctc
     7021 gctggcggtt tacccgtcat cacagccggt gtttatggat ctcgccaaaa aaggtgtggt
     7081 agcagatttg attggcgcag gcgcaatcat cagaaccgca ttctgtggcc catgctttgg
     7141 cgcgggcgat acgccaatca acaacggttt gagtattcgc cacaccacgc gtaacttccc
     7201 gaaccgcgaa ggctctaagc cagccaatgg gcagatgtca gcggtggcgt tgatggacgc
     7261 tcgttctatc gctgcgactg cggcaaacgg tggctattta acctctgcca gcgaacttga
     7321 ttgctgggac aacgtgccgg agtacgcctt cgatgtaacg ccgtataaaa accgtgttta
     7381 tcagggcttt gtgaaagggg caactcagca accgctgatt tacggaccga acattaaaga
     7441 ctggccggaa ttgggtgcgc tgactgacaa tatcgtcctg aaagtgtgct cgaagatcct
     7501 cgacgaagtc accaccaccg acgaactgat tccttccggt gaaacctctt cttatcgttc
     7561 aaatccgatt ggtctggcgg ggaatgtcag cgagctgaca gaggtgtttg cgcgcattaa
     7621 gcagattgct ggtcaggagc atattgatcc gctgcaaact gaaattggca gcatggtcta
     7681 tgcggtgaaa ccaggcgatg gttctgcgcg tgaacaggcg gcgagctgcc agcgtgtgat
     7741 tggcggtctg gcgaatattg ccgaggagta cgcgactaaa cgctaccgtt ctaacgtcat
     7801 caactggggg atgttaccgc tgcagatggc ggaagtgcca acctttgaag tgggggatta
     7861 catttacatc cctggcatta aagcggcgct ggataatccg ggtacgacgt ttaaaggtta
     7921 tgtgatccat gaagatgcgc cggtaacgga aattacgctt tacatggaaa gcctgacggc
     7981 tgaagagcgc gagattatca aggcgggtag tttgattaac ttcaataaaa accgtcagat
     8041 gtaaaaagcg ccatgtgaat gtaggtcgca ttcggcactt attgtcggat gcgatgctgg
     8101 cgcatcttat ccgacctaca aatcgcatcg aaccgtaggc cggataaggc gtttacgccg
     8161 catccggcaa atagttaata gctcttactt cttcgcctct gcaaccactt tgctgcccac
     8221 gccgcggtta ttgtattccc acatgcggtt gtagttagtg tcattcagat tgcgctgtat
     8281 ttcgtcgtta tcatctacgc tgccggtatt acccgcaaac ggacgattag agatcaccgc
     8341 atcggcccac ggtttagccg tgttaaaacc ttcgttgatg gcgctatcac ggatcaccac
     8401 ctgaccgttg gtattagcat caacatccag cgagcggccc agttgcgcca cgccatcacc
     8461 ggaagcattg aaacggctgt ttacggcgag gaaaccgtaa tagatgttag acagcgtagc
     8521 cggtgcaaac acatatgctt cttgctgggt acgtgagttc accacgcgga attcggtgtt
     8581 atcgaacacc actgcgccgc gaccagaaac gatatccaca tccccttcaa tatagctgtt
     8641 ggtcaccagc gtacgcggct gacggttggt ttccagacgg ttctgcacac cgctgttggt
     8701 gacaaagaag gtgttctgac gaccgagaat gttaacgttg ttaatctgta cttggtcacc
     8761 gtcagtacgc agtgctaccg ccggatggtt acctgcatct acgctatcgc ccagcgtgtt
     8821 ttcgatggtc agattttgca gttgcagtcc attgttttgt gaccagaaga ccgcagaaca
     8881 cagaacaccg atactgtcgc tgcgtttgct ctggcagcta tcgtacatat accacgctgg
     8941 tttacctggc atatatttgc cgcgcgggtt gacgtcgtga cgccagtcgg cagggctcat
     9001 gcccccatca agggaaagcc caatcttcac atcaatcggt ttttcacctg taccgtacag
     9061 agtgattcca cctggagcag cagggacata caccgttccc tgatactccc caggcatcac
     9121 ggcaatatac tggcgcttgt tggtgcgctt gataattgcc gcatctaccg ccgcctgaat
     9181 cgtggtatgc gttacacctt gagtacccgc cgggccgaca acaaagtcag gttgcgcagg
     9241 cagggttatc ggggacggat tccacgctgc agcacctggt gtcagggatg caaaatagtg
     9301 ttgcgcatcg aaattcagcg cttcttttgc cgacagaatc gggcgagaag aggtaccagg
     9361 cgcagtttga tcagaaggac gttgatcggg tggtgttgag ctacaggcgg tcagcgtcac
     9421 gccaaaagcc aatgccagcg ccagacggga aactgaaaat gtgttcacag gttgctccgg
     9481 gctatgaaat agaaaaatga atccgttgaa gcctgctttt ttatactaag ttggcattat
     9541 aaaaaagcat tgcttatcaa tttgttgcaa cgaacaggtc attatcagtc aaaataaaat
     9601 cattatttga tttcaatttt gtcccactcc ctgcctctgt catcacgata ctgtgatgcc
     9661 atggtgtccg acttgtgccc gagaagatgt tgagcaaact tatcgcttat ctgcttctca
     9721 tagagtcttg cagacaaact gcgcaactcg tgaaaggtag gtggatcccc ttcgaaggaa
     9781 agacctgatg cttttcgtgc gcgcataaaa taccttgata ctgtgccgga tgaaagcggt
     9841 tcacgacgag tagatgcaat tatggtttct ccgccaagaa tctctttgca tttatcaagt
     9901 gtttccttca ttgatatccc gagagcatca acatgcaatg ttgtagggat ggcaattttt
     9961 acgcctgttt tgctttgctc gacataaaga tatccatcta cgatatcaga ccacttcatt
    10021 tcgcataaat caccaactcg ttgcccggta acaacagcca gttccattgc aagtctgagc
    10081 caacatggtg atgattctgc tgcttgataa attttcaggt attcgtcagc cgtaagtctt
    10141 gatctcctta cctctgattt tgctgcgcga gtggcagcga ccgggtttgt tgttatatgg
    10201 ccttcagcta ttgcctctcg gaatgcatcg ctcagtgttg atctgattaa cttggctgat
    10261 gccgccttgc cctcgtctat gtatccattg agcattgccg caatttcttt tgtggtgatg
    10321 tcttcaagtg gagcatcagg cagccccctc cttattgctt taattttgct catgtaattt
    10381 atgagtgtct tctgcttgat tcctctgctg gcgaggattt tttcgtagcg atcaagccat
    10441 gaatgtaacg taacagaatt atcactgttg attctcgctg tcagaggctt gtgtttgtgt
    10501 cctgaaaata actcaatgtt ggcctgtata gcttcagtga
//
LOCUS       DAJTQY010000121         9942 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.121, whole genome shotgun sequence.
ACCESSION   DAJTQY010000121 DAJTQY010000000
VERSION     DAJTQY010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9942
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.121"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            446..1504
                     /gene="citC"
                     /locus_tag="OO349_003424"
     CDS             446..1504
                     /gene="citC"
                     /locus_tag="OO349_003424"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HCR5868371.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISKSVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTCVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAAPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1519..1815
                     /gene="citD"
                     /locus_tag="OO349_003425"
     CDS             1519..1815
                     /gene="citD"
                     /locus_tag="OO349_003425"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HCR5868372.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILHARLEALLARASGIPALPWEDCQ"
     gene            1812..2720
                     /gene="citE"
                     /locus_tag="OO349_003426"
     CDS             1812..2720
                     /gene="citE"
                     /locus_tag="OO349_003426"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HCR5868373.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHAHRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2731..4263
                     /gene="citF"
                     /locus_tag="OO349_003427"
     CDS             2731..4263
                     /gene="citF"
                     /locus_tag="OO349_003427"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HCR5868374.1"
                     /translation="MTQKIEQSQRQERVAAWNRRTECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMNVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVNRVGDAAKIGAGTTRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAV
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDRQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            4267..4818
                     /gene="citX"
                     /locus_tag="OO349_003428"
     CDS             4267..4818
                     /gene="citX"
                     /locus_tag="OO349_003428"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HCR5868375.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHAWLKHHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHRVTALRALATKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            4793..5671
                     /gene="citG"
                     /locus_tag="OO349_003429"
     CDS             4793..5671
                     /gene="citG"
                     /locus_tag="OO349_003429"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HCR5868376.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSRLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMATNGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPTDLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            5722..7184
                     /gene="citT"
                     /locus_tag="OO349_003430"
                     /pseudo
     CDS             5722..7184
                     /gene="citT"
                     /locus_tag="OO349_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
     gene            7298..8104
                     /gene="rna"
                     /locus_tag="OO349_003431"
     CDS             7298..8104
                     /gene="rna"
                     /locus_tag="OO349_003431"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HCR5868377.1"
                     /translation="MKAFCRNAALLAVSLLPLSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKTDFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            8333..8743
                     /gene="rnk"
                     /locus_tag="OO349_003432"
     CDS             8333..8743
                     /gene="rnk"
                     /locus_tag="OO349_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HCR5868378.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(8974..>9942)
                     /locus_tag="OO349_003433"
     CDS             complement(8974..>9942)
                     /locus_tag="OO349_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR5868379.1"
                     /translation="GDCFFCRLQQYAACENTNAGKGAALNKKQIPAPAALFGYSHLYG
                     GVPGGQAEYVRVPKGNVGPFKVPPLLSDDKALFLSDILPTAWQAAKNVQIQQGSSVAV
                     YGAGPVGLLTIACARLLGAEQIFVVDHHPYRLRFAADRYGAIPINFDEDSDPAQSIIE
                     QTAGHRGVDAVIDAVGFEAKGSTTETVLTNLKLEGSSGKALRQCIAAVRRGGIVSVPG
                     VYAGFIHGFLFGDAFDKGLTFKMGQTHVHAWLGELLPLIEKGLLKPEEIVTHYMPFEE
                     AARGYEIFEKREEECRKVILVPGAQSAEAAQKAVSGLVNAMPGGTI"
BASE COUNT         2344 a         2675 c         2505 g         2418 t
ORIGIN      
        1 gatacgcagc ggaaatgcca gtctttggaa aaatgcaaac tgtttattct cgttaagctg
       61 caacattgtt ttccttgcgg gttgtcccga gcctctttga cggatgtaat taaaatgttg
      121 aatcaaaata catagatatt aattatgatc gtggcaaatt ttattcatta aatcaattaa
      181 atcacttaaa cctgcgagtc atttcgtaat tacattaatt aaaaaactta aaaccattaa
      241 ataaataaaa aaccactaac tctatgtgaa ataaatcaaa atttcacgcc gaaatactcc
      301 ttaggatgta tagcgaaagg agaaaaagat atacctcgat cacccccctt tctcccaagt
      361 gaaaataaaa ggttatcagt ttgcaacatt gaacaacatt cgttgcaaat cgataacaac
      421 atgcaccttc aggatactat ttattatgtt cggcaatgat attttcaccc gcgtaaaacg
      481 ttcagaaaat aaaaaaatgg cggaaatcgc ccaattcctg catgaaaatg atttgagcgt
      541 tgacaccaca gtcgaagtat ttattaccgt aacccgcgat gaaaagctta tcgcgtgcgg
      601 tggaattgcc ggaaatatta ttaaatgcgt tgctatcagt aaatccgttc gcggtgaagg
      661 actggcgctg acattagcca ctgaactgat aaacctcgcc tatgagcggc acagcacgca
      721 tctgtttatt tataccaaaa ccgaatacga ggcgctgttc cgccagtgcg gtttttccac
      781 gctgacctgc gtacccggcg tgatggtgct gatggaaaac agcgccacgc gactgaaacg
      841 ctatgccgaa tcgctgaaaa aatttcgtca tccagggaac aagattggct gcattgtgat
      901 gaacgccaat ccctttacga atggtcaccg ttatctgatt caacaagctg cggcacagtg
      961 cgactggttg catctgtttt tagtcaaaga agattcttca cgcttcccct atgaagaccg
     1021 gctggatctg gtgttaaaag gcaccgccga tattccacgc ctgactgtgc atcgcggctc
     1081 agaatacatc atctcccgcg ctacgttccc ttgctacttc attaaagaac agagcgtcat
     1141 taaccattgt tacaccgaaa ttgatctgaa gattttccgt cagtacctcg ctcccgcgct
     1201 tggtgtaact caccgctttg tcggtactga acccttttgt cgcgttaccg cccagtacaa
     1261 ccaggatatg cgctactggc tggaaacgcc gactatctcc gcagcgccca tcgaactggt
     1321 tgaaattgag cggctgcgtt accaggagat gccgatatcc gcttcccggg tacgtcaact
     1381 gctggcgaaa aacgatctca cggctatcgc gccgctggtc cctgcagtca cgctgcatta
     1441 tttgcagaac ctgcttgagc actcccgcca ggacgcggca gctcgtcaaa agacccccgc
     1501 atgagaaaca ggtgaaaaat gaaaataaac cagcccgccg ttgcaggcac ccttgagtct
     1561 ggggatgtga tgatacgcat cgccccactc gatacgcagg atatcgacct gcaaatcaat
     1621 agcagcgttg agaaacagtt tggcgatgca attcgcacca ccattctgga cgttctcgcc
     1681 cgctacaacg tgcgcggcgt acagctgaat gtcgatgaca aaggcgcact ggactgcatt
     1741 ttacatgcac gactggaagc cctgctggca cgcgccagcg gtatcccggc tctgccatgg
     1801 gaggattgcc aatgatttcc gcttcgctgc aacaacgtaa aactcgcacc cgccgcagca
     1861 tgttgtttgt gcctggtgcc aatgccgcga tggtcagcaa ctccttcatc tacccggctg
     1921 atgccctgat gtttgacctc gaagactccg tggcattacg tgaaaaagac accgcccgcc
     1981 gcatggttta tcacgcgctg caacatccgc tgtatcgcga tattgaaacc attgtgcgtg
     2041 tcaacgcgct ggattccgaa tggggtgtta acgacctgga agccgtcgtt cgcggtggtg
     2101 cggacgttgt gcgtctgccg aaaaccgata ccgctcagga tgttctggat attgaaaaag
     2161 agatcctgcg tatagaaaaa gcctgtggtc gtgaacccgg cagcaccggc ctgctggcgg
     2221 cgattgaatc tccgctgggc attacccgcg cagtggaaat cgctcacgct tccgagcgtt
     2281 tgatcggtat cgccctcggt gcagaagact atgtgcgcaa cctgcgtaca gaacgctccc
     2341 cggaaggaac tgaactgctg ttcgcacgct gttccatttt gcaggccgcg cgctctgcgg
     2401 gtattcaggc gttcgatacc gtctattccg acgctaacaa cgaagccgga ttcctgcaag
     2461 aagccgccca catcaaacag ctgggctttg acggcaaatc gctgatcaac ccgcgtcaga
     2521 ttgatctgct gcacaacctc tacgcaccga cccagaaaga agtggatcac gcccaccgcg
     2581 tcgtagaagc cgctgaagcc gccgctcgcg aaggcctcgg cgtggtttcc ctgaacggca
     2641 agatggtgga cggtccggtt atcgatcgcg cccgtctggt gctctcccgt gcagaacttt
     2701 ccggcatccg cgaagaataa ggcaatcaaa atgacgcaga aaattgaaca atctcaacga
     2761 caagaacggg tagcggcctg gaatcgtcgc actgaatgcg atcttgccgc tttccagaac
     2821 tcaccaaagc aaacctacca ggctgaaaaa gcgcgcgatc gcaaactgtg cgccaacctg
     2881 gaagaagcga ttcgtcgctc aggtttacag gacggcatga cggtttcctt ccatcacgct
     2941 ttccgtggcg gtgacctgac cgtcaatatg gtgatgaacg tcatcgcgaa gatgggcttt
     3001 aaaaacctga ccctggcgtc cagctccctg agtgattgcc atgcgccgct ggtagaacac
     3061 attcgccagg gcgtggttac ccgcatttat acctctggcc tacgcggtcc actggcggaa
     3121 gagatctccc gtggtctgct ggcggaaccg gtgcagatcc actctcacgg cggtcgtgtg
     3181 catctggtac agagcggcga actgaatatc gacgtggctt tcctcggcgt cccgtcctgt
     3241 gatgaattcg gtaatgccaa cggctacacc ggtaaagcct gctgcggctc cctcggctat
     3301 gcaatggttg atgccgacaa cgcaaaacag gtcgtgatgc ttaccgaaga actgctgcct
     3361 tatccgcata atccggcaag cattgagcaa gatcaggttg atttgatcgt caaagttaat
     3421 cgcgttggcg atgctgcaaa aatcggcgct ggtactaccc gtatgaccac taacccgcgc
     3481 gaactgctta ttgcccgtag cgctgcggat gtgattgtca actctggcta cttcaaagaa
     3541 ggtttctcca tgcaaaccgg caccggcggc gcttcgctgg cggtaacccg tttcctggaa
     3601 gacaaaatgc gtagccgcga tattcgcgcc gacttcgccc tcggcggtat taccgcgacg
     3661 atggttgatc tgcacgaaaa aggtctgatc cgcaaactgc tggatgtgca gagctttgac
     3721 agccatgctg tgcaatcgct ggcccgtaac cccaatcaca tcgaaatcag cgccaaccag
     3781 tacgctaact ggggttcgaa aggcgcatcg gttgatcgtc tcgacgtggt ggtactgagc
     3841 gcgctggaaa ttgacaggca gttcaacgtt aacgtgctga ccggttctga cggcgtactg
     3901 cgtggcgctt ccggtggtca ctgcgatacc gcgattgcct ctgcgctttc catcatcgtc
     3961 gcgccactgg tacgcggtcg tattccgact ctggtggata acgtgctgac ctgcatcacc
     4021 ccgggctcca gtgtcgatat tctggtcaca gaccacggta tcgcagttaa cccggcacgt
     4081 ccggaactag cagaacgtct gcaggaagcg ggcattaaag tggtttccat tgagtggctg
     4141 cgcgaacgtg cgcgtctgct gaccggtgaa ccacagccga ttgaattcac cgaccgcgtc
     4201 gttgccgttg tgcgttaccg cgatggctcg gtgatcgatg ttgtgcatca ggtgaaggaa
     4261 taagccatgc acctgcttcc tgaactcgcc agccaccatg cggtgtcaat tcccgagctg
     4321 ctcgtcagcc gggatgaaag gcaagcacgg caacacgcct ggctcaagca ccatcctgtt
     4381 ccactggtct cctttaccgt ggttgcgcct gggccgatta aagacagcga ggtcacacgc
     4441 cgaattttta atcatcgcgt gacagccttg cgtgccttag ctacaaagca gggctggcaa
     4501 attcaggagc aggctgcact ggtttccgcc agcgggccgg agggcatgtt gagcattgcc
     4561 gccccggctc gcgacctcaa gctcgccacc attgagcttg aacatagtca tcctctcggg
     4621 cggttatggg atatcgatgt cctgacgccc gaaggcgaaa ttctctcccg ccgcgactat
     4681 tcactgccgc ctcgccgctg cctgttgtgc gagcaaagcg cagccgtctg cgcgcgtgga
     4741 aaaacccatc aactgaccga tttactcaac cgcatggagg cactgctgaa cgatgtcgat
     4801 gcctgcaacg tcaactaaaa ccacaaagct tgcgacgtca ttaatcgatg agtacgccct
     4861 gctgggctgg cgcgccatgc tgactgaagt caatctgtca ccgaaaccag gcctcgtgga
     4921 tcgcattaac tgcggtgcgc acaaagatat ggcgctggaa gatttccacc gcagcgcgct
     4981 ggcgattcag ggctggttac cccgtttcat tgaatttggt gcctgtagtg cagaaatggc
     5041 accggaagcg gtactccacg gattacgccc aattggtatg gcttgcgaag gtgatatgtt
     5101 ccgcgccact gcgggcgtaa acacgcataa aggcagcatt ttttctttag ggctgctatg
     5161 tgcggcaatt ggccgtttgc ttcagctcaa ccagccggtt acgccaacaa ccgtttgttc
     5221 tacggcggca agtttctgcc gtggcctgac cgatcgcgaa ctgcgtacca ataattcacg
     5281 actgacggca ggtcaacggt tgtaccagca gcttggcctt accggcgcac gcggtgaagc
     5341 cgaagcaggt tatccactgg tgatcaacca cgccttgccg cattacctca ctctgctgga
     5401 tcaggggtta gatcctgaac tggcattgct cgataccttg ctcctactga tggctaccaa
     5461 cggcgatacc aacgttgcat cgcgcggtgg cgaggggggc ctgcgctggc tacagcgcga
     5521 ggcgcaaaca ttattgcaaa aagggggcat tcgaaccccc accgatctcg attatctccg
     5581 gcagttcgac agggagtgta tcgaacgaaa tctcagtcca ggcggcagtg ccgacctgct
     5641 gatccttacc tggtttttag cacagattta attatttaag cacttgataa atttggaaat
     5701 attaattttc ggagaacccg tatgtcttta gcaaaagata atatatggaa actattggcc
     5761 ccactggtgg tgatgggtgt catgtttctt atccctgtcc ccgacggtat gccgccgcag
     5821 gcatggcatt acttcgctgt gtttgtggca atgattgtcg gcatgatcct cgagccaatt
     5881 ccggcaacag cgatcagttt tattgcggtt actatttgcg ttattggcag taattacctg
     5941 ctctttgatg ccaaagaatt agctgaccca gcgtttaatg cgcaaaaaca ggcgctgaaa
     6001 tggggcctgg ctggtttttc cagcaccacg gtatggctgg tatttggcgc atttattttt
     6061 gcattagggt atgaagtttc cgggttaggt cgtcgcattg cccttttcct ggtgaaattc
     6121 atggacaaac gcacgctgac gttgggttat gcgattgtca ttatcgacat tctgctggca
     6181 ccgtttacac cgtccaacac cgcgcgtacc gggggtacgg tttttccggt cattaaaaac
     6241 ctgccgccgt tgtttaaatc attcccgaac gatccgtccg cgcgtcgtat tggcggctat
     6301 ttgatgtgga tgatggtcat tagtaccagt ctgagttcgt ccatgtttgt caccggtgcg
     6361 gaccaaacgt gctgggtctg gagttcgtca gcaaaattgc cggtatccag attagctggt
     6421 tgcagtggtt cctctgcttc ctgccggttg gggttatctt gcttatcatt gcgccgtggc
     6481 tttcctacgt gctgtacaaa ccggaaatca cacacagtga agaagtggca acctgggcgg
     6541 gtgatgaact aaaaaccatg ggtgcgctga cacgcagaga gtggacgctg attggccttg
     6601 tattgctcag cttaggtttg tgggtatttg gcagtgaagt cattaatgct actgcggttg
     6661 gtctgctggc agtttcgcta atgctggctc tgcacgttgt gccgtggaaa gacattaccc
     6721 gctataacag cgcatggaac acgctggtca acctggcaac tctggttgtg atggctaacg
     6781 gcctgactcg ttctggtttt attgactggt tcgccggtac catgagtacg cacctggaag
     6841 gattctcacc aaacgcaacg gtgattgtac tggttctggt gttctacttt gcacactacc
     6901 tgtttgccag cctgtctgcg cacaccgcaa ccatgctgcc ggttattctg gccgtcggta
     6961 aaggtattcc gggcgtacca atggaacaac tgtgtatcct gctggtgctg tctatcggta
     7021 tcatgggctg tctgacgccg tatgcaaccg gtcctggggt gattatttac ggctgtggct
     7081 atgtgaaatc aaaagattac tggcgtcttg gcgcaatctt cggggtgatt tacatctcta
     7141 tgttgctgtt ggttggctgg ccgattctcg ccatgtggaa ctaatacgtt cgccaaacgc
     7201 tgaataaatc atcacgcccg ccaggtaagc ccacctggcg ggctttttta tgatttaata
     7261 gatagtattc agtgtatgta tgagttccac acccattatg aaagcattct gccgtaacgc
     7321 cgcgttgctc gcggtttctc tgcttccctt atcttctgcc aacgccgtag cgttgcaggc
     7381 aaaacagtat ggcgattttg atcgctatgt cctggccctc tcctggcaaa ccggattttg
     7441 ccagagtcaa cacgatcgaa atcgtaacga acgagatgaa tgtcgcctgc aaaccgaaac
     7501 gaccaacaaa actgattttc tgaccgtaca tggtctgtgg ccaggattgc ctaaatcggt
     7561 tgctgcccgt ggtgttgatg aacgccgctg gatgcgcttc ggttgcgcta ctcgcccaat
     7621 cccgaatcta ccagaagcac gcgccagccg aatgtgttca tcgccggaaa ccgggttatc
     7681 actggaaacg gccgctaaac taagtgaagt catgccagga gctggcggac gttcctgcct
     7741 ggaacgctac gaatatgcca aacacggtgc ctgctttggt tttgatccgg acgcatactt
     7801 cggtacgatg gtacgcctga atcaagaaat taaagagagc gaagccggaa aattccttgc
     7861 ggataattac ggtaaaacag tgagccgccg tgactttgac gccgcctttg ccaaaagctg
     7921 gggaaaagag aacgtgaaag cagttaagct aacgtgccag ggtaaccctg cgtatttgac
     7981 tgaaattcag atctcgatca aagctgacgc catcaacgct ccgctttctg caaactcatt
     8041 cttgccacaa cctcacccag gtaactgtgg caaaaccttt gtgattgata aagcgggtta
     8101 ttaatcctct gatttacaaa acacgctaat tcgtcttcca taaccatgcc gattaagaat
     8161 tatacataat gcgctgcatt gaggaattcc ctgaatgtga cgcaaatcac ttcaaagagt
     8221 gagcaacagt cagtatcatc atgtgagtta aaccctcgcc gcctgacggt gagggttttc
     8281 ttttgggtat gtttatctgc gacactcgca gtaccgacga catggagtaa aaatgtccag
     8341 accaactatc atcattaacg acctggatgc cgaacgcatc gatattctgc tggagcaacc
     8401 cgcctatgct ggtttgccaa tcgccgacgc gttaaacgca gagttggatc gcgcccaaat
     8461 gtgttcgcca gaagagatgc cacacgacgt ggtgacaatg aacagccggg ttaaattccg
     8521 caatcttagc gatggcgaag tgcgtgtgcg cacgctggtg tatccggcaa aaatgaccga
     8581 tagcaatact cagctttccg ttatggctcc ggtaggtgcc gcactgctgg ggctgcgcgt
     8641 tggcgattcc attcactggg aacttccggg cggcgttgca acccaccttg aagtgctgga
     8701 actcgaatac cagccagaag ctgctggcga ctacctgctt taatcccatc tgcacaagcg
     8761 ataactcgct ttgcccagag gatgcactcg catcctcttt acgcaaaatc agcaattttt
     8821 cctggttctc actgttcact tttgctgcaa aattattccg tttcgtttgt gggaataacc
     8881 atggcagaag cattttacat cctgatagga tttctgatta tggcggcgat tatcgtcatg
     8941 gctgtccttt acctcgaaaa ccactcctga cgatcatatt gttccccccg gcatcgcatt
     9001 caccagacct gaaaccgcct tctgcgccgc ctctgcgctt tgtgcaccgg gtaccagaat
     9061 caccttacgg cactcctctt cacgtttttc gaaaatctca tatccccggg cggcctcttc
     9121 aaacggcata tagtgggtaa caatttcttc tggtttcagt aatcctttct caattaacgg
     9181 taataattct cccagccatg cgtgaacgtg ggtctgtccc attttaaacg tcaacccttt
     9241 atcaaaggcg tcgccaaaca ggaaaccgtg aataaatcca gcgtagacgc ccggtacgct
     9301 aacaatgccg ccacgcctga ccgccgcaat acactggcgc aacgctttac cgctgctgcc
     9361 ctccagtttc aggttagtca gcaccgtttc cgtggtgctg cctttcgctt caaaaccgac
     9421 ggcgtctatt actgcatcca cgccccggtg acctgccgtt tgttcaataa ttgactgtgc
     9481 cggatcgcta tcttcatcga aattaatcgg gatcgcgccg tagcggtcag cggcgaaacg
     9541 caagcggtag ggatgatgat caacaacaaa aatctgttcc gcaccgagca accgtgcaca
     9601 ggcgattgtc aacaatccca caggaccagc accatagact gcaacgcttg aaccttgttg
     9661 gatctgcaca ttttttgctg cctgccatgc cgttggcaga atatcagaaa ggaaaagggc
     9721 tttatcatct gaaagcaaag gtggtacttt aaacggcccc acattccctt tcgggacgcg
     9781 gacatattcc gcctgcccgc caggaacgcc accatacagg tgactataac caaacaatgc
     9841 cgcgggagca ggtatctgtt ttttattgag cgcagcgcct ttacccgcat tggtattttc
     9901 gcaggcggca tattgctgta atcgacagaa aaaacagtcg cc
//
LOCUS       DAJTQY010000122         9928 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.122, whole genome shotgun sequence.
ACCESSION   DAJTQY010000122 DAJTQY010000000
VERSION     DAJTQY010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9928
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.122"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            <1..339
                     /locus_tag="OO349_003434"
     CDS             <1..339
                     /locus_tag="OO349_003434"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5868380.1"
                     /translation="LSSNNEYDLFKNRETSLWKRKVPESAALAMTGTVYKLFGNRLLN
                     ARNQLDKNLLEMAMQMVYGKDMVTKSGDILIDIQLNKDGSLQSKHYTLDVGEVINSYN
                     IDPLCECFKY"
     gene            527..1642
                     /locus_tag="OO349_003435"
     CDS             527..1642
                     /locus_tag="OO349_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5868381.1"
                     /translation="MFNHTTKTADNIEHNLQLSFLVGSRPSITNKYSAKDVGPDILAL
                     PVNVYHADFKIKINNAVEKASSIVLCAVQKNLHAARKVLSGIIGNVPKNNKPKTIIFD
                     LRFLSAIKQKVFLGSEKPLFVKHTAMMREACKELPQSVEYVPLACEAHSHRSVALAKT
                     VAAPIMATGVGLALMYATSSGPYYAQSLSGSPDIEMIKNRMAELFNQSDISVRNKYSP
                     AFDKWNELVDKLNHERNTVPFACLTTILATAINETLEGDTNVAVVMGCKSAKDRTISI
                     VLGNSMLQTLLEKRLADSGEIENLFDQQGYFSCDSLTPEELMMLKDLFDIRVLHVSNK
                     FNVGLQGNINTDVLQDSFFKNVDFIHEYNSYTVGMGV"
     gene            2321..2539
                     /locus_tag="OO349_003436"
     CDS             2321..2539
                     /locus_tag="OO349_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HCR5868382.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            2757..2999
                     /locus_tag="OO349_003437"
     CDS             2757..2999
                     /locus_tag="OO349_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5868383.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(3329..4258)
                     /gene="fdhE"
                     /locus_tag="OO349_003438"
     CDS             complement(3329..4258)
                     /gene="fdhE"
                     /locus_tag="OO349_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HCR5868384.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQKVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(4255..4890)
                     /gene="fdoI"
                     /locus_tag="OO349_003439"
     CDS             complement(4255..4890)
                     /gene="fdoI"
                     /locus_tag="OO349_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCR5868385.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHQNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(4887..5789)
                     /gene="fdoH"
                     /locus_tag="OO349_003440"
     CDS             complement(4887..5789)
                     /gene="fdoH"
                     /locus_tag="OO349_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HCR5868386.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(5802..8852)
                     /gene="fdnG"
                     /locus_tag="OO349_003441"
     CDS             complement(5802..8852)
                     /gene="fdnG"
                     /locus_tag="OO349_003441"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(8265..8267),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCR5868387.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNHEYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            9046..9879
                     /gene="fdhD"
                     /locus_tag="OO349_003442"
     CDS             9046..9879
                     /gene="fdhD"
                     /locus_tag="OO349_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HCR5868388.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGRLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         2490 a         2396 c         2543 g         2499 t
ORIGIN      
        1 ttgtcgagta ataatgaata tgacctgttt aaaaatagag aaacctcact gtggaaacgc
       61 aaagtaccag aaagcgcagc gctggcaatg acgggaacag tatataaatt atttggtaat
      121 cgcttactca acgcccgcaa tcagttagat aaaaatctcc tcgaaatggc tatgcaaatg
      181 gtttatggca aagatatggt gacaaagtcc ggggatatat tgattgatat acaattaaat
      241 aaggatggca gtctccagag taagcactac acacttgatg taggtgaagt tataaacagt
      301 tataatattg accctctttg tgaatgcttc aagtattaat caaacttact tacatgataa
      361 aaatcctggt gacgttatta gcctgtatgg cactttagat aacattcccc ttgtagttaa
      421 ggaatgcacg ggtaaaaatc atcaaattaa tgttaaagcc aaattgccac aagaacgaag
      481 tgaaaaagtt gaggatatgt gtgaaagaat gaaaggtggc atctctatgt ttaaccacac
      541 cacaaaaaca gctgataata tagagcataa tttgcagcta agttttctcg tgggttcacg
      601 tccgtcgatc accaataaat actcagcaaa agatgtaggt ccagatatac ttgcattgcc
      661 cgtcaacgta tatcatgcgg attttaaaat taaaatcaat aatgcagtgg agaaggcttc
      721 atcaattgtg ttatgtgctg tgcaaaaaaa tctccatgca gcaaggaagg ttcttagcgg
      781 gataatagga aatgtgccaa aaaataataa accaaaaaca attatttttg atttgcgttt
      841 tcttagtgca attaagcaga aagttttttt gggcagtgaa aaaccgttgt ttgtaaagca
      901 taccgcaatg atgagggagg catgtaagga attaccgcag tcagtggaat atgttccttt
      961 agcctgtgaa gcgcatagcc atcgcagcgt cgcccttgcc aaaaccgtgg ctgcgcccat
     1021 catggcgaca ggtgtgggct tggcattaat gtatgcaaca tcatcaggac cgtattatgc
     1081 gcaaagtctt tctggttcgc cggatatcga gatgataaaa aatcgcatgg ctgaattgtt
     1141 taaccagtcg gatatttctg tgcgcaacaa atatagcccg gcttttgata aatggaatga
     1201 actggtagat aaattaaatc acgaaagaaa tacagttccg tttgcgtgtt tgaccaccat
     1261 tttagcgacg gcgataaatg aaactcttga gggggatacg aatgttgccg ttgtgatggg
     1321 gtgtaaatct gcgaaagatc gcacgatatc cattgtcctt ggcaactcga tgttgcaaac
     1381 actccttgaa aaacgcctcg ccgatagtgg agaaatagaa aatctttttg atcaacaggg
     1441 atattttagt tgcgattcat taacacccga agaattaatg atgttaaaag acctttttga
     1501 tattcgagtt cttcatgtct ctaacaaatt caatgttgga ttgcaaggta atatcaacac
     1561 tgatgtttta caagattcgt tttttaaaaa tgtcgatttc attcatgaat ataactcgta
     1621 taccgtaggg atgggagtct gacgagtaaa actcattagg attatcagaa ttcggacaat
     1681 gcattgcaaa aatgacatac aaagagagat gtgtgcaggt acttagccgc gaggggcatg
     1741 gcgaagccat acagaaagta ccagtattca gaactccagt gaaagcggca tagtggccgc
     1801 ctgctcagga gttttgctat gacccgtaat cgtgctgaac gtcgccaccc tatgatgcga
     1861 ttaaaaaaac tcatcgtcgc gctgatgatt gacgtcaatg ctatcggatg atgccggggg
     1921 tgttccggaa ggaattcctt gtttcccgaa ctgttaccaa caaaattata acgatgagca
     1981 agtaaagacc ggttggtggc agtactgacc ggtctgtgat gaaagattaa tttcacctta
     2041 aatcctggtg ctgttaggat gctgacgttt ttcggctaca ccattcagat tatgcaaata
     2101 aatatccctg aatataatat attcatagca cccataactc atcctgggcg ataactaaac
     2161 catttttgag taataaatga aacaactgca acgctcttat tatttctgga aaacgcctcg
     2221 caaaaacaaa atgacatgca aaaatccgca tttttttaaa tgcttttccg gcgtaatttt
     2281 cgcacataca ttaatgagta atatatgtaa ggtggtggta atggcgatga atacggtttt
     2341 tcttcattta tcagaagagg caattaaacg gctgaacaag cttcggggct ggcgtaaggt
     2401 ttcacgatct gcaattttac gcgaagcggt tgagcaatat ctggagcggc agcaatttcc
     2461 ggtgcgtaaa gcaaagggag gcaggcaaaa gggggaggta gttggtgttg atgatcagtg
     2521 taaggagcat aaggaatgaa aaaaattttt cattcttgaa tataaaaaac agatgccctt
     2581 attctggtat taatacaagg ctgttttact tgaacttata ataactgcaa ctgttacatc
     2641 gtatctggaa aacgcctcgc aaaaataaaa aatgatgcgt aaatgagctt tctttaaaga
     2701 ttcggcaagg gtacttttct ctccatagat tcaaaaatga tattggcgag atatttatga
     2761 actctctggc aggtatagat atgggcagaa ttttactcga tttatcgaat gaggtgatta
     2821 agcaacttga tgatctcgag gtgcagcgta atcttcctcg tgcggaccta ttaagggaag
     2881 cggtagatca atacctgata aatcaatcgc aaacagcaag aaccagtgtt cctggcatct
     2941 ggcaagggtg tgaggaagat ggtgtcgaat atcagcgtaa gctgcgcgag gaatggtaat
     3001 gggaaaaaat gatgtcaatc agattgctga caacgtgcgc gttgttcatg ccggatgcgg
     3061 cgtgaacgcc ttatccggcc tacaaagtca tgcaaattca atatattgaa gtttattggt
     3121 aggcctgata agcgcagcgc atcaggcaat catacgcttg tcatcagtga catacccata
     3181 accttatcga tgtgttggag ggaagaacga ggcaaacaga caattgcggc atgatcttga
     3241 atgggtaacg cggaatacga gagatggtgt cgtcacaccc tatgagctgt aatccgggcg
     3301 acctgcgcca cccggaaaac gtcagagatt actctccttc acccggaaac aggaacgggt
     3361 taatggaact acgggcgtag ccttcttgct ccattcgcgc gtccagtacc agagaggcga
     3421 ggtcatctgc cacggcttca actttcggtt ctttttcctg ataaagaatc ttcaggtaag
     3481 tgccgcagtc atcgcagctt tcggctttaa tcgcggcctg ttcgtcatcc agcgaccagt
     3541 aatgcagttt gccgctctgc tcgcagttgc tgcattttac gcgcactacg tgccattcgg
     3601 tttcacacag gttgcagtgc aggtaacgca gaccctgagt ggtgccaatt tgcaccatgc
     3661 tggacaccgg catagagcca cataccgggc aatattgacg ttgttcgccg tattcagcgc
     3721 gggctttgcc ggggatcaga ttggccatct gcgcccagta gagcgacagc gcagcccaga
     3781 taaacggcgc tttatcgctg ctgacggacg agaaatcaga ggcaaacagc gcgctggcca
     3841 tatcttccag ctcctgagtc gatgccttct ccagattctc aatcactgcc agcgccgggc
     3901 cgctcatttc aggtttcagc tcagcaatta gcgccatcag cagcttttgc cagtgcttat
     3961 cacgcggcag aacgtgaata tccagcgggg gcttgccctg tgcgctggct tctttaatgc
     4021 gcgcggtcag atccatctcc agcggatggt cgtacagcac cactttctgg gcgtgggcga
     4081 taagcgcagc aaagcgcagg taatcaccca gcggattatt ttctgccagc tcgcgcagac
     4141 gctcggcgcg gcggttgtat aaattcttga gccgagggaa caataacggc ggaatcatat
     4201 ccgccgtacg tttctcgctc gaacccagct catcttgcgg gattatgcga atactcattc
     4261 agctttcttt tccgttgtct tgcggacctc acggtaccag cgcgggtgat gtttcttcgc
     4321 ccaggcgctg gtaacccatc cttccaccat ggcggtaatc gtgcctttca cccaaagggc
     4381 ggcgtagata tgcaccatga taaccacaat taacgccact gcggcaaatg aatgcagcat
     4441 taacgcgaat cggatcaccg ggattgagaa agcaggcgca aaataaggac gccagataat
     4501 cacgccgctc accagcagca gaaccaggaa aataatcgcc gcccagaaaa cgcatttctg
     4561 accgaagtta taacgcccgg tgtcacctac ttcctcgttg acgacgatct tacgaatatt
     4621 cttcgcccaa aagatatcat cccgattgat taggttttga tgccagtaac ggaaaaacat
     4681 gatgatgaac gaggcaaaca taaccacgcc gacaaacggg tgcagaattc gcgccagctg
     4741 cggtgtgccc atgatttgca tcaaccagtt gaaggacggg aacaaaaagc ccagcccgct
     4801 caccgccgcc aggatgaagc agaaggcggt gatccagtgg ttgatacgtt ccggcgcggt
     4861 gtagcgcacg atggtgtcac gtcgtttcat ttgcgctcct cgtctttctc ttcgtgcaga
     4921 ttattctctt cctcatccgc acggttcgga ccgacaccca cgtagtggaa gatactggcc
     4981 gcaaaggtag ccgcaaagcc aacagccgcg agcggtttcc agatgccttt ccagaatttc
     5041 acggtttcgc tgatttccgg gttctccggc aagccatgat acagatttgg cttgtcagca
     5101 tggtgcagca cgtacatgac gtgtgtacca ccgacgcctg ccggatcgta cagacccgca
     5161 ttgtcgtaac cgcgggtttt cagctcagca acgcgctcgc tcgccagcgt tttcatcgac
     5221 tctttcgtgc cgaagtgaat cgcgcccgtt gggcaggtct tcacgcaggc tggttcttgc
     5281 ccaaccacca cgcggtcaac gcacagcgta catttgtaga cgcggttgtc ttccgggttg
     5341 aggcgcggaa tgtcgaacgg acagcccgca atgcaataac cgcagccaat gcactgctcg
     5401 gactggaagt cgacgatacc gttggcatac tgaataattg caccttccgc cgggcacgct
     5461 ttcaggcagc ctggatcgga acagtgcata cagccgtctt tgcggatcag ccattccagt
     5521 ttgtcgttct gctccacttc cgagaagcgc attaccgtcc acgatttggc gcttaaatca
     5581 ttggggttgt cgtacacccc aatgttattg ccgacggtat cgcgaatgtc gttccactct
     5641 gaacaagcca cctgacaggc tttacagccg atacaggtgg taacgtcgat gagtttcgcc
     5701 acttcttcct ggaagtcccg cgcctgaggc gcgggggtca gaccgttagt cgcggaacga
     5761 cgaatgatat cttgagattg ataagccata tgtcgtctcc gttacacctt ttccacattc
     5821 acaaggaagg acttaaactc cggcgtctgc gtgttcgcat caccaacgaa tggcgttaaa
     5881 gtattggcaa taaagccttt tttagctacg ccttcatagc cccagtgaat cggaataccg
     5941 atggtatcga tatctttgcc gttcgctttc agcgtgcgaa tacgtttggt caccaccgct
     6001 ttggctttga tatagccacg gttggaggag actttcacgg tatcgccctg ggcaatgcca
     6061 agtttattcg ccagcgactc cccgatttcc acaaactgct ctggctgcaa aatcgcgttc
     6121 aacagcgcgt gtttggtcca gtagtggaag tgctcggtca gacgataggt ggttccgaca
     6181 tacgggaact tatcggcttt acccaatgct tcggcgtcgt ctttaaagat acgtgcagct
     6241 gggttcgaga taacgtttgg atgcagcggg ttagttccca gcggcgtttc aaacggctcg
     6301 tagtgttccg ggaacggacc ttccgccatc ttatcgaggg caaacagacg ccccatgcct
     6361 tcctgctgca tgataaacgg cccgacgccg ctacccggag gcgctgcgct gtagtccgga
     6421 atatcccagc cggtccactt agtgccgtcc catttcagca actgacgctt cggatcccac
     6481 gggttaccct gcggatctgc ggaggcgcgg ttatacagaa tgcggcggtt aagcggccat
     6541 gcccatgccc agcccagcgt gttaccgagg ccagacggat cggcgttatc acgacgcgcc
     6601 atctggttgc cttccggcgt ccagctaccg gcgaaaatcc agcagccaca ggaggttgta
     6661 ccgtcatcgc gcaattgggc gaacgagcta agttgttggc ctttcttgac gataaccgca
     6721 ccggttgccg gatcggtaat atcggccagc gccttaccgt tgctctccat cgctacttct
     6781 tccgacttcg gctcatgcgg aatggcgtag ttccaggtca tgttcagcac ctgatccggg
     6841 ttcgcgccac cctgttcggc atacatcttg cgcaagcgca ggaagatacc ggagaggatc
     6901 tcaccgtcag tcagcgcaat ccccggggcg tccgcacctt tccagtgcca ctgcaaccag
     6961 cgacctgagt taacgattga accgttctct tctgcgaagc aggtcgatgg cagacggaac
     7021 acttcggtct ggatcttcga cgagtcaact tcgttcagct caccgtggtt ctgccagaag
     7081 ttagaggttt cagtgttcag tgggtcgatg gtgacgagga acttcagttt cgacagacaa
     7141 ccgatcactt tgtttttgtt cgggaatgag gcaacagggt taaagccctg gcagatatag
     7201 ccattgacct tgccctcttt catcatctcg aagtattgca ggacgtcgta gcctttatcc
     7261 cacttcggca accagtcaaa gccccagcta ttttccgccg tcgctttatc accaaagaag
     7321 gccttcatca tagagacgaa gaatttcggg tagttgcccc agtagtttac ctggccttcc
     7381 agcagcggtt ttggcgtgtt ggcggtaagg taggtttgca gatcggtctg cttctcgctt
     7441 ggcagcgtca tgtaacctgg cagactctgc gacagtagcc ccaggtccgt cagcccctga
     7501 atattggagt gaccgcgcag ggcgttaacg ccgccgccgg ccatccccat attgccgagc
     7561 agcagctgga tcatcgccat cgtacgaatg ttttgcgcac caacggagtg ttgcgtccag
     7621 ccgagggcat acaggaacga ggcagtttta tcgtgagcac tggtttctgc gatgtattcg
     7681 cagactttca ggaacgcgtc ttttggcgta ccacagatgt tttcaaccac atctggcgtg
     7741 tagcgggaaa cgtgctgttt cagcaagttc cacacgcagc gcggatgttg cagcgtggta
     7801 tcgcgtttgg cgaagccgtt ttcgtccagt tcataagtcc aggaggattt atcgtacttg
     7861 cgtttttccg cgtcgtagcc ggtgaacagg ccatcttcaa agccgtaatc ctcacgcacg
     7921 atcaggctgg cgttggtata ggcttcggtg tattcgtggt tgaatttttc attgttcagc
     7981 aggtacagca atacgcctga caggaaagca atgtcagtac cggaacgaat aggggcatag
     8041 tagtcagcta ccgccgccgt acgcgtaaag cgaggatcga tcacaatcag cttcgcgccg
     8101 ttgtgaattt tggcttccat cgcccagcgg aacccgaccg ggtgagcttc agcggcgtta
     8161 ccgcccatca ccacgacgag gttggcgttc ttgatgtcga cccagtggtt ggtcatcgca
     8221 ccgcgaccaa atgttggagc aagacttgct accgttggtc cgtgtcagac acgcgcctgg
     8281 ttgtcgaccg cgagcatacc cagcgcgcgg gagaattttt gcgttaaata gccggtttcg
     8341 ttactcgacg cggaagcaca cagcatcccg gtggagagcc agcggttaac agtcacgcct
     8401 tcggcgtttt gcgcaatgta gttagcatcg cggtcttctt tcatcagttt ggcgatgcga
     8461 tcaaacgcct cttcccaact gatttgctgc catttatcag aacctggcgc acggtattcc
     8521 ggaaacttca gacggctttc ggagtggatg aaatccacca ggccagcgcc tttcggacaa
     8581 agtgcaccgc ggttgaccgg gtgatccgga tcgccttcga tatggaagat agatgctttg
     8641 gcgttttttg ctccgtcacc gaggctgtac atcaacagcc cacagcctac ggaacaatag
     8701 gtgcaggtat tacgggtttc gcgggtgcgc agcagtttat actgccgggt ttccgcgagt
     8761 gctacgctgg gtgcaaaacc cagtgccgct gccgtggtgc ctgccatacc gccagcgcag
     8821 atcttaaaga actgccttct gctgacctgc atggattgct ccttgtttcg acattgtcac
     8881 gtcccattta cattcgcttg ctgcgtgtgc agggagtggg agttattttt ctttgcggaa
     8941 ggggccgcaa aggtccagaa ttggctcaat ttccctccat ctaggaaggg tttgtaacag
     9001 aataccataa tgttggtgtg tatgttctta tctggttaag agaaagtgaa aaaaacacag
     9061 cgaaaagaaa tcgaaaatgt gacaaatatc acaggtgttc gtcaaattga gttatggcgg
     9121 cgtgatgatt tacaacaccc acggttagat gaggtcgcgg aagaagttcc cgttgcgctg
     9181 gtctacaacg gcatttcgca tgtggtgatg atggcgtcgc ccaaagacct tgagtacttt
     9241 gcgctcggtt tttcgctttc cgaggggata atagaaagtc cgcgcgatat cttcggcatg
     9301 gatgtcgttc cttcctgtaa tggtcttgaa gtacaaattg agctttccag ccgccgcttt
     9361 atggggttga aggagcgccg ccgggcgctg gcgggacgta cgggctgtgg cgtatgcggc
     9421 gtggagcaac ttaatgacat cggaaaaccg gtgcagccgc taccgttcac ccagacgttt
     9481 gatctcaaca aactggatga tgcattacgt catctcaacg attttcagcc agtggggcga
     9541 ctgactggtt gtactcacgc cgctgcctgg atgttgccat ctggcgaact ggtcggcggg
     9601 catgaagacg tgggtcgcca tgtggcgctg gacaaactgt taggccgccg gtcacaagaa
     9661 ggggaaagct ggcagcaagg tgcggtactg gtttccagtc gtgccagtta tgaaatggtg
     9721 caaaagtcgg cgatgtgcgg cgtagagatt ttgtttgcgg tgtctgccgc gaccacgctt
     9781 gctgtagaag tggctgagcg ctgtaatctg acactggtag gtttttgtaa accgggtcgg
     9841 gcaacggttt atacccatcc gcagcgtttg agcaattaat taaaataagt cctgcgatat
     9901 atattaaatt gcaggactta ttcatttc
//
LOCUS       DAJTQY010000123         9910 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.123, whole genome shotgun sequence.
ACCESSION   DAJTQY010000123 DAJTQY010000000
VERSION     DAJTQY010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9910
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.123"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(151..891)
                     /gene="pflA"
                     /locus_tag="OO349_003443"
     CDS             complement(151..891)
                     /gene="pflA"
                     /locus_tag="OO349_003443"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HCR5868389.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(1097..3379)
                     /gene="pflB"
                     /locus_tag="OO349_003444"
     CDS             complement(1097..3379)
                     /gene="pflB"
                     /locus_tag="OO349_003444"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCR5868390.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLSYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(3434..4291)
                     /gene="focA"
                     /locus_tag="OO349_003445"
     CDS             complement(3434..4291)
                     /gene="focA"
                     /locus_tag="OO349_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HCR5868391.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWCQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            complement(4697..6457)
                     /locus_tag="OO349_003446"
     CDS             complement(4697..6457)
                     /locus_tag="OO349_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory protein YcaO"
                     /protein_id="HCR5868392.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLSENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLETEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEDFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            6587..7213
                     /locus_tag="OO349_003447"
                     /pseudo
     CDS             6587..7213
                     /locus_tag="OO349_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
     gene            7412..8500
                     /gene="serC"
                     /locus_tag="OO349_003448"
     CDS             7412..8500
                     /gene="serC"
                     /locus_tag="OO349_003448"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HCR5868393.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            8571..9854
                     /gene="aroA"
                     /locus_tag="OO349_003449"
     CDS             8571..9854
                     /gene="aroA"
                     /locus_tag="OO349_003449"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HCR5868394.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGLSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLDSNDIVLTGEPRMKERPIGHLVDALRQGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
BASE COUNT         2522 a         2370 c         2480 g         2538 t
ORIGIN      
        1 gaagaacttc acgcgagcta ttatcagtcg ttattatctc aggtacggga tatggtagat
       61 gcactataag atgtgttaaa aacgctgtag cagaatgaag cgcggaataa aaaagcggca
      121 actcaataaa gttgccgctt tacgaggaaa ttagaacatt accttatgac cgtactgctc
      181 aagaatgcct ttcacgcgtt ccatggtctc tttcttcggt ggtttaacac catcgagttt
      241 gtattcttca cccatcgcca cccatttgtg tttgcccagt tcgtggtagg ggagaagttc
      301 gattttctca acgttaccca tatcacgggt aaattcaccg aggcgatgtg ccgaatcgtc
      361 atcgtcagac cagcctggga caacaacgta gcggatccac accttcacat ttttgttcgc
      421 cagatattta gcgaactcca gcgtgcggtg gttggaaact ccaaccagat tttggtggat
      481 ctcgtcgttc atctgtttga gatcgagcat taccaggtcg gttacttcca gcagttcatc
      541 aatcaccgga tcgtaacgac gaacaaaacc gttggtatcc agacaggtat gaatgccttc
      601 ttttttacag gcgcggaacc agtcacgaac aaactcagct tgcaggattg cttcaccgcc
      661 ggatgcggta acgccgccgc cggaagcgtt cataaagtgg cgataggtca ccacttcctt
      721 catcaaatct tcaacggtaa cttctttacc gccatgcgtg tcccaggtgt cgcggttatg
      781 acaatacagg cagcgcatca ggcagccctg gaaaaaggtg ataaagcgaa tacctgggcc
      841 gtctacggtt ccacaggatt caaaggagtg aatgcgacca ataactgaca ttgcggtgtt
      901 tctccagatg tggcccatct gaggccgtgt tggtgcgcag ctcgaaggct acgtcgagtc
      961 tgttttggca gtcaccttaa agtatagata gctgacaaaa aaggctctcg tgctaaaaaa
     1021 ggccccactt tcgtggagcc tttattgtac gctttttact gtacgctttt tactgtacga
     1081 tttcagtcaa atctaattac atagattgag tgaaggtacg agtaataacg tcctgctgct
     1141 gttctttagt cagcgagttg aaacgtactg cgtagccaga tacacggatg gtcagctgcg
     1201 gatatttttc cgggttttcc atcgcgtcga gcagcatttc acggttcatc acgttaacgt
     1261 tcaggtgctg accaccttcg atggatgctt cgtggtggaa gtaaccatcc atcagaccag
     1321 ccaggttggt cttacgaact tcgtcgtctt tacccagtgc gttcggaacg atagagaagg
     1381 tgtaggagat accatcttta gcgtaagcaa acggcagttt agcaacggaa gtcagagacg
     1441 ctacagcacc tttctggtca cgaccgtgca tcgggttagc acccggtccg aacggcgcgc
     1501 cagcacgacg accgtctggg gtgttaccag ttttcttacc atacacaacg ttagaagtga
     1561 tggtcagaac agactgagtc gggatagcgt cacggtaggt gtgcagtttc tgaattttct
     1621 tcatgaaacg ttctaccagg tcaacagcca ggtcatctac acgcggatca ttgttaccaa
     1681 actgcgggta ttcgccttcg atttcgaagt cgatagccag accgtcttcg tcacgaatcg
     1741 gtttaacttt cgcatatttg attgcagaca gggagtcagc agcaacggac agaccagcga
     1801 taccacacgc catggtgcgg ataacgtcac ggtcgtgcag cgccatcaga gaggcttcgt
     1861 agctgtactt gtcgtgcatg tagtggatga tgttcagtgc agtgatgtac tgtttagcca
     1921 gccagtccat gaagtgatcc atgcgctcca tcacttcatc gtagctcagg agatcgcctt
     1981 tgatcggttc agacttcgga ccaacctgca ttttcagttt ttcgtcaacg ccgccgttga
     2041 ttgcgtacag catggttttc gccaggtttg cacgcgcacc gaagaactgc atttgtttac
     2101 caacgatcat cgggcttacg cagcaagcga tagcgtagtc atcgttgttg aagtccggac
     2161 gcatcaggtc atcgttctca tactgcagag aagaggtgtc gatggacact ttagcggcga
     2221 atttcttgaa gttcagcggc agtttttcag accacagaat ggtcatgttc ggttccggag
     2281 acggccccat agtgtacagg gtgttcagga aacggaagct gtttttggta accagggtac
     2341 gaccatcgag gcccatacca ccgatagatt cggttgccca gattgggtcg ccagagaaca
     2401 gttcatcgta ttccggagta cgcaggaagc gaaccatacg cagtttcatg accaggtggt
     2461 caaccatttc ctgcgcttct tgttcggtga tcttgccagc tttcaggtca cgttcgatgt
     2521 acacatccag gaaggtggag gtacgaccga aggacattgc agcaccgttc tgagacttaa
     2581 cagcagccag gtagccgaag taagtccact ggatagcttc ctgagcgttg gtagccggac
     2641 cagagatgtc gtagccgtat ttcgcagcca tttctttcat ctgacccaga gcgcggtgct
     2701 gttcagcgat ttcttcgcgc agacggatag tctgttccag gtttacgccg ttttccagat
     2761 cagcctgtag agaggtgaac tgagcgtatt tgtctttcat caggtagtcg ataccgtaca
     2821 gcgcaacgcg acggtagtca ccgatgatac ggccacggcc ataagcatct ggcagaccgg
     2881 tcagaacacc ggatttacgg caacgcagga tgtccggagt gtaaacgtcg aacacgccct
     2941 ggttgtgagt tttacggtat tcagtgaaga tttttttgat catcgggtcc agttcgcggt
     3001 tgtacgcttt gcaggaacct tcgatcattt tgataccacc gaacgggata agagcacgtt
     3061 tcagcggagc ttcagtctgc agaccaacaa ctttttccaa cgctttattg atgtagccag
     3121 cgtcgtgaga ggtgatggtg gaagcaacag cggtgtcaaa atcaactggc gcgtgagtgc
     3181 ggttttccag tttaacacct tccattactt tgtcccacag ggtggtggtc gcttcagtag
     3241 cgccagccag gaaggactcg tcaccctcgt acggcgtgta gtttttctga atgaagtcac
     3301 ggacgtttac ttcattctgc cagtcacctt tggtaaaacc ttcccaggct gtggctaact
     3361 tttcattaag ctcggacatg taacacctac cttcttaagt ggatttttta tttactgcgt
     3421 acttcgacaa ccattaatgg tgatcgtttt cacgcaggta aatgacccag tatgtcaacc
     3481 caaccaacaa accaccgccg ataatattac cgatcgtaac cgggatcagg ttatcagtga
     3541 tgaagttcat cacggtcagg tgagaaaaat tttccggtgc agaaccgaca gcggtccaga
     3601 attccggaga tgcgaagtcg cggattacaa tacccatcgg gatcataaac atgtttgcga
     3661 tactgtgctc aaaaccgctg gcaacaaaca tcgcgaccgg cagcaccata atgaacgctt
     3721 tgtccatcag gctgcggcca gaataactca tccatactgc cagacatacc atcaggtttg
     3781 ccaggatacc aagacagacg gcctcaataa aagtatggtg cactttgtgg tcggcggttt
     3841 gtaggacgtt tagtccccat tgaccatttg cggtcatata ctcgccggaa agccacatta
     3901 aaagtacaaa cagcagtgcg ccgaccaggt tgccaaaata gacatttagc cagtttttag
     3961 ccaactgaca ccaggtgatg cgcccactcg ccttagcaac aacaatcaac actgtggaag
     4021 taaagagatc ggctccgcag acaacacaaa gaatcagccc cagagagaag caaatgccgc
     4081 caaccagttt tgccatgccg aagggcattg tgcctgtgcc agtggttgct gtgatataga
     4141 agacgaatgc gattgagatg aaaacaccgg cggtaatcgc cagatagaaa gtcttaagcg
     4201 gatgtttcgt tgctttatag acacccgcct cttcggccac tttggccatt gcagcaggaa
     4261 gtaaaagatc aaaagggttg tcagctttca cactaactct ctctttatta agtcggcgac
     4321 gagatactaa caaagcatta tagatgagaa attgatatag atcatatctc gcctggctta
     4381 taggcccgta actcgcatgg tttttatgca aatacggagt aactatttga ttatccaaat
     4441 aaaaataaat tttaaaaatt aacaaatgag ttgaattttt tccgcatcct ccgctaaaac
     4501 agttaattaa aagggagcat caggcgaata aagtaacaat atcgatcgta tttattaaat
     4561 acaaattacc gatatttaac cttataatta caattatttt attaatgcaa atatatgtaa
     4621 agcggggcat taaaaaaacg ccccgtaata taactcagac taatcattaa gcctacattg
     4681 cgtaggctat ttgattttat tttgcccaga atgctgcttt ggcgcgctgc agcttttcgt
     4741 aggccttcaa caacgactga tgtgcagcaa acgcgtgcag atcgctatct accggttgca
     4801 ggccgtaaaa cgccgcttcg ccgctcattg ccgcactggc ggcttctacg gcatctgcgc
     4861 cgtacatgcg aacaaacgca ttcagatatt gcagcggctg gcgatcttct tcctgtgcca
     4921 gtaataacaa cgtttgcagg cagcgataat agttggcgcg ttccggacta aatactgatg
     4981 agttaaactc catcgtccat tcggtccaga ccagagcctg ttccagatcg ccaccagcca
     5041 gcgccagcat ggcttttaat tcaccgatac gcagggtgta ccaaccgtta tccgacccgg
     5101 tcgccagacc caacagctca cgcacgcggg taaagtcatc aaaatcttct tcatccagtt
     5161 gctcgatgag gttcaggtaa tcttcttttt cccactcgct gcctggtagc gaaagaatcg
     5221 tttcacgtaa atggctgccc atactgttat tcgcgagcca cagatcttca gccggataaa
     5281 tatcggacat gccaggcacg ataatacggc aagcataaac gcccagatgc tcgtaatcgg
     5341 caatataaac ttctttatct tctttgttga agatagccat cagcgtagca aactcttctt
     5401 ccgtggtgcc ggagaaattc cagtccacaa acggataatc ggcatcctgc ttgaacaggt
     5461 cccaggagat taaaccgctg gaatcgataa agtgcgtttc gaggttggta tgttcagcga
     5521 cttcttcatc atcgaaggtt ggcggagtaa acacatccaa atctttcagg ccacgacctt
     5581 gcagcagctc ggtcacggta cgttccagtg ctacgccaaa atcaggatgc gcaccgaaag
     5641 aggcaaagca ggtgccgttt gcaggattaa acagtaccac gcaaatcacc ggatactggc
     5701 cgccaaggga accatcatat gcgaagatcg ggaaaccttc tgtttccagt gtttcgatcg
     5761 cttcaaccac cgctgggtaa cgcgccagca cgtctgccgg gatttccggc aagctgatgc
     5821 tttcagcaat aatgcggttt ttcacgtagc gttcgaaaac ttcggacaac ccctgaacgc
     5881 gtgcttcgtt gcgggtattg ccagcggaca taccgttaga aacgtacagg ttaccaatga
     5941 tattcatcgg aatataaacg gtctgattgt cggactggcg cgtaaacggc agaccgcaaa
     6001 taccacgatc ttcgttaccg gattgtaggt caatcagcat actgccggtc aattcattct
     6061 ccggatcgta aaatgcgcgc agacggtcat cgagcagccc ttctggcaca tcgtcatttt
     6121 cgctcagtgg gaaccatttt tcgttgggat aatgcacaaa tggaccgttg gcgatggttt
     6181 cgcccagcca gaaatcagca aaaaagtagt tggttgagag acgctcgaaa tattcaccga
     6241 gtgcagaagc cagcgccgct ttcttggttg cgcctttacc gttggtaaaa cacagtgcgc
     6301 actctttgtc gcgaatatgt acagaccaga cgttaggcac gggattcagc caggaggcct
     6361 cttcaatctg aaagccgagg tctgaaagtt tttgctggaa gcgagcgatg gaatcttcca
     6421 gagcggcatc tttgccgggg ataaatgttt gcgtcatgaa aatcacttta gtcgtacgga
     6481 aagcgcgcaa taatacgggt tttatctcaa aggcgctatc accgccgcca tgccgatgaa
     6541 ctgttgacta tgcttttagc ggataaacca cgttaagagc ataaaaatga aggcgttcga
     6601 tctccaccgt atggcatttg ataaagtgcc ttttgatttc cttggcgaag ttgcactacg
     6661 tagtctttat acctttgtac tggttttttg ttcctcaaaa tgaccggaag acgcggtgtg
     6721 cgacagatgt cgctgtttga agtgttaatc attctcacgc tgggttcagc ggcgggagat
     6781 gtggcgtttt atgatgatgt gccgatggtg ccggtactta tcgtctttat cactctggcg
     6841 ttgttatacc gcctggtaat atgatggcga gctggcctgg tcgaaactca ataactccaa
     6901 catgacggaa tttgagttct ttatggagct acgattgcgt ggcgtggagc agctggggca
     6961 ggtacgtctg gcgattctcg aaaccaacgg gcaaatcagt gtctatttct ttgaagatga
     7021 caaggtgaaa ccgggtttac ttattttacc cagtgattgt acgcagcgtt acaaagtggt
     7081 gccggagtcg gcggactatg cctgcattcg ttgtagtgaa atcattcata tgaacgcggg
     7141 ggaaaaacaa ttatgtccgc gctgtgcaaa tccagaatgg acgaaggcaa gtcgggcaaa
     7201 acgggtgacc tgacagtaaa aacatcggct ttttgctaat aatccgagag attcttttgt
     7261 gtgatgcaag ccacattttt gccctcaacg gttttactca ttgcgatgtg tgtcactgaa
     7321 tgataaaacc gatagccaca ggaataatgt attacctgtg gtcgcaatcg attgaccgcg
     7381 ggttaatagc aacgcaacgt ggtgagggga aatggctcaa atcttcaatt ttagttctgg
     7441 tccggcaatg ctaccggcag aggtgcttaa acaggctcaa caggaactgc gcgactggaa
     7501 cggtcttggt acgtcggtga tggaagtgag tcaccgtggc aaagagttca ttcaggttgc
     7561 agaggaagcc gagaaggatt ttcgcgatct tcttaatgtc ccctccaact acaaggtatt
     7621 attctgccat ggcggtggtc gcggtcagtt tgctgcggta ccgctgaata ttctcggtga
     7681 taaaaccacc gcagattatg ttgatgccgg ttactgggcg gcaagtgcca ttaaagaagc
     7741 gaaaaaatac tgcacgccta atgtctttga cgccaaagtg actgttgatg gtctgcgcgc
     7801 ggttaagcca atgcgtgaat ggcaactctc tgataatgcg gcttatatgc attactgccc
     7861 gaatgaaacc atcgacggta tcgccatcga cgagacgcca gacttcggca aagatgtggt
     7921 ggtcgccgcc gacttctctt caaccattct ttcccgtccg attgacgtca gccgttatgg
     7981 tgtaatttac gctggcgcgc agaaaaatat cggcccggct ggcctgacaa tcgtcatcgt
     8041 tcgtgaagat ttgctgggca aagcgaatat cgcgtgtccg tcgattctgg attattccat
     8101 cctcaacgat aacggctcca tgtttaacac gccgccgaca tttgcctggt atctatctgg
     8161 tctggtcttt aaatggctga aagcgaacgg cggtgtagct gaaatggata aaatcaatca
     8221 gcaaaaagca gaactgctat atggggtgat tgataacagc gatttctacc gcaatgacgt
     8281 ggcgaaagct aaccgttcgc ggatgaacgt gccgttccag ttggcggaca gtgcgcttga
     8341 caaattgttc cttgaagagt cttttgctgc aggccttcat gcactgaaag gacaccgtgt
     8401 ggtcggcgga atgcgcgcgt ctatttataa tgccatgccg ctggaaggcg ttaaagcgct
     8461 gacagacttc atggttgagt tcgaacgccg tcacggttaa tgccgaaatt ttgcttaatc
     8521 cccacagcca gcctgtgggg tttttatttc tgttgtagag agttgagttc atggaatccc
     8581 tgacgttaca acccatcgct cgtgtcgatg gcactattaa tctgcccggt tccaagagcg
     8641 tttctaaccg cgctttattg cttgcggcat tagcacacgg caaaacagta ttaaccaatc
     8701 tgctggatag cgacgacgtg cgccatatgc tgaatgcatt aacagcgtta gggttaagct
     8761 atacgctttc agccgatcgt acgcgttgcg aaattatcgg taacggcggt ccattacacg
     8821 cagaaggtgc cctggagttg ttcctcggta acgccggaac ggcaatgcgt ccgctggcgg
     8881 cagctctttg tctggatagc aatgatattg tgctgaccgg tgagccgcgt atgaaagagc
     8941 gcccgattgg tcatctggtg gatgcgctgc gccagggcgg ggcgaagatc acttacctgg
     9001 aacaagaaaa ttatccgccg ttgcgtttac agggcggctt tactggcggc aacgttgacg
     9061 ttgatggctc cgtttccagc caattcctca ccgcactgtt aatgactgcg cctcttgcgc
     9121 cggaagatac ggtgattcgt attaaaggcg atctggtttc taaaccttat atcgacatca
     9181 cactcaatct gatgaagacg tttggtgttg aaattgaaaa tcagcactat caacaatttg
     9241 tcgtaaaagg cgggcagtct tatcagtctc cgggtactta tttggtcgaa ggcgatgcat
     9301 cttcggcttc ttacttcctg gcagcagcag caatcaaagg cggcactgta aaagtgaccg
     9361 gtattggacg taacagtatg cagggtgata ttcgctttgc tgatgtgctg gaaaaaatgg
     9421 gcgcgaccat ttgctggggc gatgattata tttcctgcac gcgtggtgaa ctgaacgcta
     9481 ttgatatgga tatgaaccat attcctgatg cggcgatgac cattgccacg gcggcgttat
     9541 ttgcaaaagg caccaccacg ctgcgcaata tctataactg gcgtgttaaa gagaccgatc
     9601 gcctgtttgc gatggcaaca gaactgcgta aagtcggcgc ggaagtggaa gaggggcacg
     9661 attacattcg tatcactcct ccggaaaaac tgaactttgc cgagatcgcg acatacaatg
     9721 atcaccggat ggcgatgtgt ttctcgctgg tggcgttgtc agatacacca gtgacgattc
     9781 ttgatcccaa atgcacggcc aaaacatttc cggattattt cgagcagctg gcgcggatta
     9841 gccaggcagc ctgaatgaac aacgggcaat aaatagccaa atctttcttt atcaaaacgt
     9901 cggcacattg
//
LOCUS       DAJTQY010000124         9626 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.124, whole genome shotgun sequence.
ACCESSION   DAJTQY010000124 DAJTQY010000000
VERSION     DAJTQY010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9626
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.124"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(16..999)
                     /gene="kdgT"
                     /locus_tag="OO349_003450"
     CDS             complement(16..999)
                     /gene="kdgT"
                     /locus_tag="OO349_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HCR5868395.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(1259..1879)
                     /gene="sodA"
                     /locus_tag="OO349_003451"
     CDS             complement(1259..1879)
                     /gene="sodA"
                     /locus_tag="OO349_003451"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HCR5868396.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            2164..3198
                     /gene="rhaT"
                     /locus_tag="OO349_003452"
                     /pseudo
     CDS             2164..3198
                     /gene="rhaT"
                     /locus_tag="OO349_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
     gene            complement(3195..4061)
                     /gene="rhaR"
                     /locus_tag="OO349_003453"
     CDS             complement(3195..4061)
                     /gene="rhaR"
                     /locus_tag="OO349_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HCR5868397.1"
                     /translation="MAEVATVAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDF
                     CELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKL
                     NLDWQGAIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVPFANEMAELLFGQ
                     LVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQ
                     FRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMT
                     PSQWRHLNSQKD"
     gene            complement(4117..4953)
                     /gene="rhaS"
                     /locus_tag="OO349_003454"
     CDS             complement(4117..4953)
                     /gene="rhaS"
                     /locus_tag="OO349_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HCR5868398.1"
                     /translation="MTVLHSVDFFPSCNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            5241..6710
                     /gene="rhaB"
                     /locus_tag="OO349_003455"
     CDS             5241..6710
                     /gene="rhaB"
                     /locus_tag="OO349_003455"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HCR5868399.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMPQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPDEMCSEIQAACRETAQPIPGSDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIELMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            6707..7966
                     /gene="rhaA"
                     /locus_tag="OO349_003456"
     CDS             6707..7966
                     /gene="rhaA"
                     /locus_tag="OO349_003456"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HCR5868400.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPRQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKAILSQRG"
     gene            8208..9032
                     /gene="rhaD"
                     /locus_tag="OO349_003457"
                     /pseudo
     CDS             8208..9032
                     /gene="rhaD"
                     /locus_tag="OO349_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312856.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
     gene            9042..9356
                     /gene="rhaM"
                     /locus_tag="OO349_003458"
     CDS             9042..9356
                     /gene="rhaM"
                     /locus_tag="OO349_003458"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HCR5868401.1"
                     /translation="MIRKAFVMQVNPDAHEEYQSRHNPIWPELEAVLKSHGVHNYAIY
                     LDKARNLLFAMVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
BASE COUNT         2352 a         2515 c         2489 g         2270 t
ORIGIN      
        1 attaaggaag caggtttatt tcaccgtacc taaaatttcg attttcgctg ctctggcttt
       61 gactttacgt gaccagatag aggtaagaat tggcaccaga atcgaagtca caatgaccgc
      121 cgtcgctacc agcgaagttg ctgccggagc catcggttta aacgcaggca ccatttctgc
      181 aatcagcaca ggtgtcgcta ccgcggcccc tgcggaactg gaagcggcaa ttccggcagt
      241 gccatcgcca ccgccaatca atttatcggc gataatcaac ggaataccgg tcacgataat
      301 tactgccaca cccaacagga tccccagcaa accagtctgg gcaatcacag tcaaatcaat
      361 ggtattgccc agcgcgaagg caaagaatgg aatcagcgtt tgcaccgctt tgctgaaaaa
      421 ttctcgtaat tcagggtcaa gattccccag ggcaaagccc accaggaacg gtaatactgc
      481 gccgacgaaa acatgcggtt caaacgaggc aatcccggca gtgcccagaa taatcatcgt
      541 catgagcggc ccggactcca acgacatcaa cacaaatgcc ccagcttctt ctttcgtacc
      601 gtactgctgc atgatggaag cgtaaagtcc gccgttggtc atatccatcg ccgccaccag
      661 cgccagcgtt gaaagtccgg caaagaatcc aacttcaaca ccatgttccg gaataatgcg
      721 cgaggcaatt gccgcaacca cccacgcgac ggcaatttta gttaccacca gcgtaccgga
      781 tttacgcagt accgttcccg tcgcgcttaa ttttattgac gcccccatgc aaaaaaacca
      841 caccgccaga atgggcaccg taccggtaat catcccgttg gtgaatgatc caaaatattt
      901 ccccgccccc ggcgagaagg tgtggcacag tgcgccaagg aatagcggga cgagcatcat
      961 cccccccggg attttctcaa tcgagcgttt tatctgcatt tccatcacct gtcggcatat
     1021 ttttaagggc tgatgcgtaa ttcctcaatg gagcatcagt gttgatggaa acgaagatag
     1081 acattcattt aatcaataaa agtgatcaaa atcacatcaa ttgaaacgct gttttatttt
     1141 tataagacca ttgtttttat ttttaaattg atcacaaaaa aaccaccgcc gttggcgatg
     1201 gttcattttt taagctgata tgcggcctat acgcctcatt gcagcaggcg gcaaatgatt
     1261 attttttcgc cgcaaaacgt gccgctgctt cgtcccagtt caccacgttc cagaactctt
     1321 tgatgtagtc cggacggcgg ttctggaatt tcaggtagta agcatgttcc cacacatcca
     1381 ggcccagaat cgggaagccg gaagcgccag aaatagcttc acccatcagc ggagaatcct
     1441 ggttagcagt agaaaccacc gccagtttat cgcctttcag caccagccat gcccagccgg
     1501 aaccaaagcg ggaagctgcc gctttttcaa attctgcttt gaagttatca acggagccga
     1561 agtcacgttc gatagccgct ttcaggtcac cctgcagggt ggtgcctttt ttcagacctt
     1621 tccagaacag gctgtggtta gcgtgaccgc cagcgttgtt gcgcagtacg gttttcttgt
     1681 ctgctggcag ctggtccagt ttggtgatca gctcttcaac cggcaggttg gcaaattctg
     1741 gcaggctttc cagcgccgcg ttggcgttgt ttacgtaggt ctgatggtgt ttggtgtggt
     1801 ggatttccat ggtctgctta tcgaagtgcg gttccagggc atcgtaagca tacggcaggg
     1861 atggcagggt atagctcata ttcatctcca gtattgtcgg gcggccgatt gttaatgccg
     1921 cgtaagcagt tggttcatta tagttaatta aatgatattg aaaatgatta tcaatgccgt
     1981 acttttcgta agggtatggt tttgcaggaa aatgcccgag atgtgaagca aatcacccac
     2041 ttaatgccgt gattgccagt aaatcgacaa cggcggcaac aggcgaaagg ttaatcgaca
     2101 gcacgatttt tacactcatc tcgtcggaga tgtgaagcga cgaaaaatga tgaggataag
     2161 aagatgagta acgcgattac gatggggata ttttggcatt tgatcggcgc ggccagtgca
     2221 gcctgttttt acgctccgtt caaaaaagta aaaaaatggt catgggaaac catgtggtca
     2281 gtcggtggga ttgtttcgtg gattattctg ccgtgggcca tcagcgccct gttactaccg
     2341 gatttctggg cgtattacag ctcgtttagt ctctctacgc tgctgcctgt ttttctgttc
     2401 ggcgctatgt gggggatcgg taatatcaac tatggcctga ccatgcgtta tctcggcatg
     2461 tcgatgggaa ttggcatcgc cattggcatt acgttgattg tcggtacgct gatgacgcca
     2521 attatcaacg gcaatttcga tgtgctgatt aacaccgaag gcggacgcat gacgttactc
     2581 ggcgttctgg tggcgctgat tggcgtaggg attgtaactc gagccgggca gttgaaagag
     2641 cgcaagatgg gcattaaagc cgaagagttc aatctgaaaa aagggctggt gctggcggtg
     2701 atgtgcggca ttttctctgc cgggatgtcc tttgcgatga acgccgcaaa accgatgcat
     2761 gaagccgctg ccgcacttgg cgtcgatcca ctgtatgttg ctctgccaag ctatgttgtc
     2821 atcatgggcg gcggcgcgat cattaacctc ggtttctgtt ttattcgtct ggcaaaagtg
     2881 aaggatttgt cgctaaaagc cgacttctcg ctggcaaaac cgctgatcat tcacaatgtg
     2941 ttactctcgg cactgggcgg gttgatgtgg tatctgcaat tctttttcta tgcctagggc
     3001 cacgcccgca ttccggcgca gtatgactac atcagttgga tgctgcatat gagtttctat
     3061 gtattgtgcg gcggtatcgt cgggctggtg ctgaaagagt ggaacaatac aggacgccgt
     3121 ccggtaacgg tgttgagcct cggttgtgtg gtgattattg tcgccgctaa catcgtcggc
     3181 atcggcatgg cgaattaatc tttctgcgaa ttgagatgac gccactggct gggcgtcatc
     3241 ccggtttccc gggtaaacac caccgaaaaa tagttactat cttcaaagcc acattcggtc
     3301 gaaatatcac tgattaacag gcggctatgc tggagaagat attgcgcatg acacactctg
     3361 acctgccgca gatattgatt gatggtcatt ccagtctgct ggcgaaattg ctgacgcaaa
     3421 acgcgctcac tgcacgatgc ctcatcacaa aatttatcca gcgcaaaggg acttttcagg
     3481 ctagccgcca gccgggtaat cagcttatcc agcaacgttt cgctggatgt tggcggcaac
     3541 gaatcactgg tgtaacgatg gcgattcagc aacatcacca actgcccgaa cagcaactca
     3601 gccatttcgt tagcaaacgg cacatgctga ctactttcat gctcaagctg accgataacc
     3661 tgccgcgcct gcgccatccc cacgctacct aagcgccagt gtggttgccc tgcgctggcg
     3721 ctaaatcccg gaatcgctcc ctgccagtca agattcagct tcagccgctc cgggcaataa
     3781 ataatattct gcaaaaccag atcgttaacg gaagcgtagg agtgtttatc gtcagcatga
     3841 atataaaaga gatcgccacg ggtaatgcga taaggacgat cgttgagtac atgcagacca
     3901 ttaccgcgcc agacaatcac cagctcacaa aaatcatgtg tatgttcggc aaagacatct
     3961 tgcggataac ggtcagccac agcgactgcc tgctggtcgc tggcaaaaaa atcatctttg
     4021 agaagtttta actgatgcgc caccgtggct acctcggcca gagaacgaag ttgattattc
     4081 gcaatatggc gtacaaatac gttgagaaga ttcgcgttat tgcagaaagc catcccgtcc
     4141 ctggcgaata tcacgcggtg accagttaaa ctctcggcga aaaagcgtcg aaaagtggtt
     4201 actgtcgctg aatccacagc gataggcgat gtcagtgacg ctggcctcgc tgtggcgtag
     4261 tagatgtcgg gctttcatca gtcgcaggcg gttcaggtat cgctgaggcg tcagtcccgt
     4321 ttgctgctta agctgccgat gtagcgtacg cagtgaaaga gaaaattgat ccgccacggc
     4381 atcccaattc acctcatcgg caaaatggtc ctccagccag gccagaagca agttgagacg
     4441 tgatgcgctg ttttccaggt tctcctgcaa actgctttta cgcagcaaga gcagtaattg
     4501 cataaacaag atctcgcgac tggcggtcga gggtaaatca ttttcccctt cctgctgttc
     4561 catctgtgca accagctgtc gcacctgctg caatacgctg tggttaacgc gccagtgaga
     4621 cggatactgc ccatccagct cttgtggcag caactgattc agcccggcga gaaactgaaa
     4681 tcgatccggc gagcgataca gcacattggt cagacacaga ttatcggtat gttcatacag
     4741 atgccgatca tgatcgcgta cgaaacagac cgtgccaccg gtgatggtat agggctgccc
     4801 attaaacaca tgaatacccg tgccatgttc gacaatcaca atttcatgaa aatcatgatg
     4861 atgttcagga aaatccgcct gcgggagccg gggttctatc gccacggacg cgttacaaga
     4921 cggaaaaaaa tccacactat gtaatacggt catactggcc tcctgatgtc gtcaacacgg
     4981 cgaaatagta atcacgaggt caggttctta ccttaaattt tcgacggaaa actacgtaaa
     5041 aaacgtcgat ttttcaagat acagcgtgaa ttttcaggaa atgcggtgag catcacatca
     5101 ccacaattca gcaaattgtg aacatcatca cgttcatctt tccctggttg ccaatggccc
     5161 attttcctgt cagtaacgag aaggtcgcga attcaggcgc tttttagact ggtcgtaatg
     5221 aaattcagca ggatcacatt atgacctttc gcaattgtgt cgccgtcgat ctcggcgcat
     5281 ccagtgggcg cgtgatgctg gcgcgttacg agcgtgaatg ccgcagcctg acgctgcgcg
     5341 aaatccatcg ttttaacaat gggctgcata gccaaaacgg ttatgtcacc tgggatgtgg
     5401 atagcctgga aagtgccatt cgccttggat taaacaaggt gtgcgaggaa gggattcgta
     5461 tcgatagcat tgggattgat acctggggcg tggactttgt gctgctcgac caacagggtc
     5521 agcgtgtggg cctgcccgtt gcttatcgcg atagccgcac caatggcctg atgccgcagg
     5581 cacaacagca actcggcaaa cgcgatattt atcagcgtag cggcatccag tttctaccct
     5641 tcaatacgct ttatcagttg cgtgcgctga cggagcaaca acctgaactt attccacaca
     5701 ttgctcacgc tctgctgatg ccggattact tcagttatcg cctgaccggc aagatgaact
     5761 gggaatatac caatgccacc accacacaac tggtcaatat caatagcgac gactgggacg
     5821 agtcgctact ggcgtggagc ggggccaaca aagcctggtt tggtcgcccg acgcatccgg
     5881 gtaatgtcat tgggcactgg atttgcccgc agggtaatga gattccggtg gtcgccgttg
     5941 ccagccatga taccgccagc gcggttatcg cctcgccgtt aaacggttca cgcgccgctt
     6001 atctctcttc tggcacctgg tcattgatgg gcttcgaaag ccagacgcca tttaccaatg
     6061 acacggcgct ggcagccaac atcaccaatg aaggcggggc ggaaggtcgc tatcgggtgc
     6121 tgaaaaatat tatgggctta tggctgcttc agcgagtgct tcaggaacgg caaatcaacg
     6181 atctcccggc gcttatcgcc gcgacacagg cacttccggc ctgccgcttc atcatcaatc
     6241 ccaatgacga tcgctttatt aatcctgacg agatgtgcag cgaaattcag gctgcgtgtc
     6301 gggaaacggc gcaaccgatc ccgggaagtg atgctgaact ggcgcgctgt attttcgaca
     6361 gtctggcgct gctgtatgcc gatgtgttgc atgagctggc gcagctgcgc ggtgaagatt
     6421 tctcgcaact gcatattgtc ggcggcggct gccagaacac gctgctcaac cagctatgcg
     6481 ccgatgcctg cggtattcgg gtgatcgccg ggcctgttga agcctcaacg ctcggcaata
     6541 tcggcatcga gttaatgacg ctggatgaac tcaacaatgt ggatgatttc cgtcaggtcg
     6601 tcagcaccac cgcgaatctg accaccttta cccctaatcc tgacagtgaa attgcccact
     6661 atgtggcgca gattcactct acacgacaga caaaggagct ttgcgcatga ccactcaact
     6721 ggaacaggcc tgggagctgg cgaaacagcg tttcgcggcg gtggggattg atgtcgagga
     6781 ggcgctgcgc caacttgatc gtttacccgt ttcaatgcac tgctggcagg gcgatgatgt
     6841 ttccggtttt gaaaacccgg aaggttcgct gaccgggggg attcaggcca caggcaatta
     6901 tccgggcaaa gcgcgtaatg ccagtgagct acgtgccgat ctggaacagg ctatgcggct
     6961 gattccgggg ccgaaacggc ttaatttaca tgccatctat ctggaatcag atacgccagt
     7021 ctcgcgcgac cagatcaaac cagagcactt caaaaactgg gttgaatggg cgaaagccaa
     7081 tcagctcggt ctggatttta acccctcctg cttttcgcat ccgctaagcg ccgatggctt
     7141 tacgctctcg catcccgacg acagcattcg ccagttctgg attgatcact gcaaagccag
     7201 ccgtcgcgtt tcggcctatt ttggcgagca actcggcaca ccatcggtga tgaacatctg
     7261 gatcccggat ggtatgaaag atatcaccgt tgaccgtctc gccccgcgtc agcgtctgct
     7321 ggcagcactg gatgaggtga tcagcgagaa gctaaaccct gcgcaccata tcgacgccgt
     7381 tgagagcaaa ttgtttggca ttggcgcaga gagctacacg gttggctcca atgagtttta
     7441 catggggtat gccaccagcc gccagactgc gctgtgcctg gacgccgggc acttccaccc
     7501 gactgaagtg atttccgaca agatttccgc cgccatgctg tatgtaccgc agttgctgct
     7561 gcacgtcagc cgtccggttc gctgggacag cgatcacgta gtgctgctgg atgatgaaac
     7621 ccaggcaatt gccagtgaga ttgtgcgtca cgatctgttt gaccgggtgc atatcggcct
     7681 tgacttcttc gatgcctcta tcaaccgcat tgccgcgtgg gtcattggta cacgcaatat
     7741 gaaaaaagcc ctgctgcgtg cgttgctgga acctaccgct gagctgcgca agctggaagc
     7801 ggcgggcgat tacactgcgc gtctggcact gctggaagag cagaaatcgt tgccgcggca
     7861 ggcggtctgg gaaatgtatt gccaacgtca cgatacgcca gcaggtagcg aatggctgga
     7921 gagcgtgcgg gcttatgaga aagcgatttt gagccagcgt gggtaaacac tgccggatgc
     7981 ggcacgagcg ccttatccgg cctacgggtc gaatgtccat gcgggctgtc accctcaccc
     8041 cggccctctc cctgaaaggg cgagggagaa aaacatagcc tcgtgcggca acggagctga
     8101 acaccgctcc ggacagtccc ctcgccccta cggggagagg gttagggtga ggggaacaag
     8161 ccgcaccatg tctaaacaag gcaacctaat ttacaggaac acagaacatg caaaacatta
     8221 cccagtcctg gtttgtccag ggaatgatca aagccaccac cgatgcctag ctgaaaggct
     8281 gggacgagcg caacggcggc aacctgacgc tgcgcctgga tgacgccgat atcgcgcttt
     8341 atcacgacaa cttccaccca caaccgcgct atattccgct cagccaaccc atgcctttac
     8401 tggcaaatac gccgtttatt gtcaccggct ccggcaaatt cttccgtaac gtccagctcg
     8461 atcctgcagc taacttaggc gtcgtaaaag tcgacagcga tggcgcgggc taccatattc
     8521 tctgggggtt aaccaacgaa gccgttccaa cttctgaact tccggctcac tttctctccc
     8581 actgcgagcg cattaaagcc accaacggta aagatcgggt gatcatgcac tgccacgcca
     8641 ccaacctgat cgccctcacc tatgtactgg aaaacgacac cgcggtcttt actcgccaac
     8701 tgtgggaagg cagcaccgaa tgtctggtag tattcccgga tggcgttggc attctgccgt
     8761 ggatggtgcc cggcacggac gaaatcggcc aggcgaccgc acaagagatg caaaaacatt
     8821 cgctggtgtt gtggcccttc cacggtgtgt tcggttccgg accgacgctg gatgaagcct
     8881 ttggtttaat cgacaccgca gaaaaatcag cacaagtatt agtgaaggtt tattcgatgg
     8941 gcggcatgaa acagaccatc agccgtgaag agttgatagc gctcggcaag cgcttcggcg
     9001 ttacaccact agccagtgcg ctggcgctgt aaggagcaaa catgatccgc aaagcctttg
     9061 tcatgcaggt aaaccccgac gcccacgaag agtatcagag tcggcataat cccatctggc
     9121 cggaactgga agcagtgttg aaatctcacg gtgtgcataa ctacgccatt tacctcgaca
     9181 aagcgcgtaa tctgctgttt gccatggtag agattgaatc tgaagaacgg tggaatgcgg
     9241 tagccagtac tgatgtttgc caacgttggt ggaaatatat gaccgatgtt atgcccgcta
     9301 acccagataa cagcccggtg agtagcgagc tgcaagaagt gttttacctg ccgtaattgc
     9361 ccaatgtgcc ggagatgcct ccggcacaat attttaaatt tcgatttgaa aaattaaaca
     9421 tattacatct cccggtaagt tatatttccc tgatacattg tgagtaaatc acaaaaataa
     9481 tgaataaccc attaatgatt catgtggttt atttaaataa ccattatgtg cattactccg
     9541 caaatctgac ctttcactct gtctcaaatt gtcattgttt accgacatat cgcctctcat
     9601 cacttaatcg ctccaccatt acaatt
//
LOCUS       DAJTQY010000125         9597 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.125, whole genome shotgun sequence.
ACCESSION   DAJTQY010000125 DAJTQY010000000
VERSION     DAJTQY010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9597
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.125"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(8..328)
                     /gene="psiF"
                     /locus_tag="OO349_003459"
     CDS             complement(8..328)
                     /gene="psiF"
                     /locus_tag="OO349_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HCR5868402.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQALKGDDRNKFMSACL
                     KKAA"
     gene            complement(447..1862)
                     /gene="phoA"
                     /locus_tag="OO349_003460"
     CDS             complement(447..1862)
                     /gene="phoA"
                     /locus_tag="OO349_003460"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HCR5868403.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVNDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTYTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH
                     AHASQVVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(1963..2223)
                     /gene="iraP"
                     /locus_tag="OO349_003461"
     CDS             complement(1963..2223)
                     /gene="iraP"
                     /locus_tag="OO349_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HCR5868404.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPCQ"
     gene            complement(2405..2608)
                     /locus_tag="OO349_003462"
     CDS             complement(2405..2608)
                     /locus_tag="OO349_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5868405.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWQCG
                     EFFSLNYKLTKRIYFIAFIYNKG"
     gene            2686..3780
                     /gene="ddlA"
                     /locus_tag="OO349_003463"
     CDS             2686..3780
                     /gene="ddlA"
                     /locus_tag="OO349_003463"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCR5868406.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(3804..4016)
                     /locus_tag="OO349_003464"
     CDS             complement(3804..4016)
                     /locus_tag="OO349_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HCR5868407.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGVLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            4276..4584
                     /locus_tag="OO349_003465"
     CDS             4276..4584
                     /locus_tag="OO349_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HCR5868408.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLAGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(4643..5737)
                     /gene="yaiW"
                     /locus_tag="OO349_003466"
     CDS             complement(4643..5737)
                     /gene="yaiW"
                     /locus_tag="OO349_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HCR5868409.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVYSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPRKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(5750..6970)
                     /gene="sbmA"
                     /locus_tag="OO349_003467"
     CDS             complement(5750..6970)
                     /gene="sbmA"
                     /locus_tag="OO349_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HCR5868410.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVAHITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            7322..8479
                     /gene="ampH"
                     /locus_tag="OO349_003468"
     CDS             7322..8479
                     /gene="ampH"
                     /locus_tag="OO349_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HCR5868411.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITHPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPRIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(8480..8983)
                     /locus_tag="OO349_003469"
     CDS             complement(8480..8983)
                     /locus_tag="OO349_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5868412.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     RATLLSMIDWNEELRSRIGVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAI
                     NRLPSEY"
     gene            complement(9071..>9597)
                     /locus_tag="OO349_003470"
     CDS             complement(9071..>9597)
                     /locus_tag="OO349_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR5868413.1"
                     /translation="LSYSHFNNDLSATMSNGTYVDGSTNSDAWGFGLKTGYDFKLGDA
                     GYVTPYGSISGLFQSGDDYQLSNDMKVDGQPYDSMRYELGVDAGYTFTYSEDQALTPY
                     FKLAYVYDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDV
                     DQDWSANVGVKYTW"
BASE COUNT         2382 a         2448 c         2283 g         2484 t
ORIGIN      
        1 tcaggtatca ggcggctttc ttgaggcagg cactcataaa cttattacga tcatcacctt
       61 tcagagcttg ttgcgttgct tgattattac attcacgcat cttttgctgc tgtggcgtca
      121 aacttttttc accaggcgca gacttgctgt tcttcaggca atcactcatg taggtcttac
      181 gagcatcccc tttcaacgcc tgcgccgtcg cctgctgatt acaggaggtc atacgctgtt
      241 gttgtggggt taaggttctc tcggcagcgc cgacggtggt taaaaaaacc agaccaaaaa
      301 gcaaggtaac cagtaatgtt attttcatag caccatccct cttcatgttt taaccatgag
      361 cgtttgcgcc cgtgatctgc cattaagtct ggttgctaac agcaaaaaaa ccacccggca
      421 gcgaaaattc actgccgggc gcagttttat ttcagcccca gagcggcttt catggtgtag
      481 aagagatcgg tctggtcggt cagtccaaca acattggcgg catgcgggcc atacgccgca
      541 atacgcaact gactgccggt atgttcttgt gaatcctctt cggaattccc gtaactcatc
      601 accatcactg cgccatcttt agtatttagc gcctgagtga ggcccggcgc tttggtatcc
      661 ggcgcaacaa cctggctggc gtgggcgtga tcagcggtga ctatgaccag cgtgttaccc
      721 tcctttttag cgaattccag cgcccgttgt acggcttcat cgagatcgac cgtctcgcca
      781 atttgcccac aagggttcgc agcgtgatcc tgtttatcga ttgacgcacc ttcaacttgc
      841 aggaaaaatc ctttctcatt tttactcaac aattcaatgg ctttgtcggt catctgtgcc
      901 agggttggta cactgtcatt acgttgcgga ttaggcgtat aggtgactgc gggcttatcg
      961 atattgccgt ggtacgttgc tttcggccct agccagcgca ctggcatatt gccgtcagca
     1021 aacaggccaa gcaggggttt ttgctgattc gcttccgtca ccgaattcag tgaggcagca
     1081 tcgttcacca actgataacc acgcgcctgt gcctgttcac gcagcgtttt tccctgccat
     1141 tcaccagcgg ttgccgtttc agcaaaggtt tttgcgccgc cgccaagcgt aacgtcggca
     1201 cgagcgttaa gcagctgttc ggtaatcgat ccttttccgc ctttttccag agcgttaccc
     1261 ggacattttt cactggtcgc gctcggaccg tagcatttgc gcgaagtcac atgtgccacc
     1321 agcgcagcgg gcgtggcatc ctgcaactct gcggtagaaa cgttaccggt cgccagacct
     1381 gcggcttttg ccatttccag aatcgttggg tgatcttttt cgtgaatatc gacgcccagc
     1441 gcgccgttat aggttttgac accggttgac caggcggttg ctgatgcagc cgagtcggtg
     1501 acgtagtccg gtttgccagt ttttttattc agcgcatagt gagtgtattg cccggtaagc
     1561 ggtaaggcat cgataccttt aaaaaagccg cccgcacctt cggcataatt acgtgcggca
     1621 gtaatttccg aatcccccat cccatcgcca atcagcaaaa taatattttt tgcaggttta
     1681 tcgctaagag aatcacgcag agcggcggtc tgatcacccg ttaagcggcg agcaccgccg
     1741 ggtgcagtaa tatcgccctg agcagcccgg ttttccagaa caggcatttc tggtgtccgg
     1801 gcttttgtca caggggtaaa cagtaacggt aagagtgcca gtgcaatagt gctttgtttc
     1861 actttatttt ctccatgtac aaatacatta aaaaataaaa acaaagcgac tataagtctc
     1921 ggccgtgaca actttatgac agctgttgaa aagattaact ttttactgac aaggatgctt
     1981 caataacttc tttacgtaat cgcgcagcag ctctgtatcg tcgtcaggaa tgctggcatc
     2041 gggctttacc tcgtacagcg ccccctctac ctgatcaatc aaccgctgtt ggtcattttg
     2101 cgccatattg cgaagcattg cagtgacgat aatctccaaa gcttctacct gcgcacacag
     2161 ttctttcgac tcttcttctt tttgggcaag cttaaataac aactcagcaa tgagattttt
     2221 catgtctgta tttccttatc caaagtatgg agaagttaac attatgtttc acaacttcat
     2281 agtgtctgga agtattattt ttatgacaaa ggaaatactt tgcgcaaata ttttttgttt
     2341 gattaccagc gaaacgtttc tcttatttaa ataattaccc acacaaaata taggctttta
     2401 attatcaacc tttattataa ataaaagcta tgaaatatat tctcttcgtt aacttataat
     2461 taagagaaaa aaactcaccg cattgccatt taagccagga ataaattagc ttcccgttca
     2521 tcatttttgt gatcttgcgc acatctttga gataacgcac tttcttcacc agtacgttgc
     2581 acgctttgtc agcagaagat tgcaacaatc tttcagaagg ccgcgaatcc cccgttttgt
     2641 cgtggtaatg tatgcacttt gtgttagacg ggataggttt ttaagatgga aaaactgcgg
     2701 gtaggaatcg tttttggtgg taaatcagcg gaacatgaag tgtctctgca atcggcaaaa
     2761 aacattgtcg atgccattga taaaagtcgc ttcgacgttg tgctgctggg cattgataaa
     2821 caagggcaat ggcacgtcag cgatgccagc aattatctgc taaatgcaga cgatcctgcc
     2881 catattgcgt tgcgcccttc ggcgaccagc cttgcgcagg tgccaggtaa acatgagcat
     2941 cagcttatcg acgcgcaaaa cggtcagccg ttgccgacgg tggatgtcat tttcccgatt
     3001 gtccacggta cgctgggcga agatggttcc ttgcagggaa tgctgcgggt cgccaattta
     3061 ccgtttgtag gttctgatgt tctggcttca gcagcctgta tggataaaga tgtcaccaaa
     3121 cgtctgctac gcgatgccgg gctgaacatt gcgccattta ttaccctgac gcgcgctaat
     3181 cgtcacaaca tcagttttgc cgaagtggag tctaaactgg ggttaccact gtttgtaaaa
     3241 ccggctaatc agggctcttc tgttggtgtc agcaaagtaa ccagtgaaga acagtacgca
     3301 attgccgtcg atctggcgtt cgagttcgat cataaagtga tcgttgagca agggatcaaa
     3361 ggtcgtgaga tcgaatgcgc agttctgggc aacgacaatc cgcaagccag cacctgtggc
     3421 gagatcgtac tcaccagcga tttctatgcc tacgacacca agtacattga cgaagatggc
     3481 gcgaaagtgg tagttccggc agccattgca ccagagatca acgataagat ccgggcgatt
     3541 gccgttcagg cttatcagac gttgggatgc gcaggcatgg cgcgtgtaga cgtgttttta
     3601 accccagaga acgaagtggt gatcaacgag atcaacacac tgcctggctt caccaacatc
     3661 agtatgtatc cgaagctgtg gcaagccagc ggtctgggtt acaccgatct gatcacacgt
     3721 ttgattgagc tggcgctgga gcgtcacgct gcggataacg cattgaaaac cacaatgtaa
     3781 tattttaacg cccgataata ttcttattgg gcgttttcag tttcttcatc gcgacgacga
     3841 ataatcaacc ccgccagcca gaaactaatc acccacgtca ccagaccgac ggcataggtc
     3901 tgccaacctt ttgcttcaaa tcccagtacc cccaccacgc cattaagaat gaatataagg
     3961 ccaatggcga acgcatagta gtgccagtca cggcggattt ttacaggcag attcatggca
     4021 gctccgttaa cgaaaaacaa catccttgca tattctgtaa caaccgtctg tggtgtagac
     4081 aacattttcg taattattaa taaatttaac ttaaggttgc cattttgtga ctcacaacga
     4141 agattcacgc ttcgggaata ttcccaacat caatttacca tgattctgat attgacaatc
     4201 taaatgacct gtcagacttg ccataagccg ctggtatcac accagaagag aagcagatga
     4261 cttccggagg tttttatggc tgatttcacc ctgtcaaaat cgctgtttag cggaaaatat
     4321 cgcaatgcct cttcaacgcc tggcaacatt gcctatgcgt tgtttgtgct gttttgcttt
     4381 tgggctgggg cgcaattgct gaacctgtta gtgcatgcgc ccggcgtcta tgagcgttta
     4441 atgcaggtcc aggaaacagg tcgcccacgg gtggaaattg gtttaggtgt cggcaccatt
     4501 ttcgggctga tcccgttttt agcaggctgc ctcatttttg cagtggtggc gctatggctg
     4561 cactggcgac atcgccgcca gtaaaattta cgttgccaga tgcggcctga aacgccttat
     4621 ctggcctgac attcctgttt gatcactgtt tcatacaacg cgcccgccgt tcgtctacgc
     4681 gcttcgcaaa ccaggccgta gtcaggttgc gggtgatttt cgggctttcc agttgaatgc
     4741 caggcaacat ttcgcggggt agtgatttac ccgttttcgc ttcggcaagt tgataaactt
     4801 tcttgtacag cgccgtctcc tcaaaagaga agctgtcacc tttctctaac tgacggcgaa
     4861 tttcgctgtc gctcatcccc agttttcccg ccagtttgcg cgttgccagt tcggttttcc
     4921 tgggttcttt actgtcgtaa cgaattaaat caccgtccag tgccagcttc acgccgctgg
     4981 ccttactgac tgcgttctga aacgcggcat tacggctggc gtaccagcca gcgttaaaat
     5041 cagcaaagcg gtatatcggt gcgctatagc tggcgggata attcagtaaa tggtaagtac
     5101 caaaccacaa cccgccgcgg cgactgaaga cttcctgacg aactgtaccg tccattttcc
     5161 acggataccc tttggtatgc tgttcggcaa aagcaatgct gacctgcatc ggcccaccgg
     5221 tgcgcaccgg attaagcgaa ccaaacagcg tctgccccat tggtaccatg ctgatcagat
     5281 cgtcaaaaat cgcgctcaat tgcttttctg tacgcaccga atctaaccgt tcgctataac
     5341 ttttgccgtt tggcgatttg atttttagcg cggtatgaac cagaaaagca ggaatgtgca
     5401 tccgttcagc acgacggtca atctcttgcc aggcgatctt gcttaagccc ggaacggccg
     5461 gatctgcctg ataatttgac tcctgctgcg ccaccgccag caccgagtag acgttttcca
     5521 gcgtcggtgc cagcccctga ctttcaaagg tggtggcgag atctttcgcc caggcatcgc
     5581 ggtctttcac gctcgcaggc atcttctgcc gcacgacgct cgctacatct atcgcttttt
     5641 cgcccttttt caacggttgc ggagcctgac tactacaccc cgctaaaacc agaaccgcca
     5701 acaatgacag cgaagataga tgattgacgc gcgacattac ttctcctttt tagctcaagg
     5761 tatgggttac ttcctgaatt ttgtcaccat ccagctcatg ttcaaagctg cgcagacgtt
     5821 tgtagataga catcaactca accagtgtgg tccatgagtt aatcaggtac tggaaagcac
     5881 cgcgaacctg accaaaaacg ttggtaatct gcgtcatcag gccgagcgta atcgtaccgg
     5941 caacaattga cggaaacagc aagaacaaac cgaaaacgtt atcgacctgc aaatagagaa
     6001 tgcgggcgat gttgaaatac atatagtgaa aatagaggcg aaaatagttt ttccgtacgg
     6061 cgctaaacag ctcgcgtacc gtaggcggcg tcgcgcgcgt ggcatcgtct tcaccataaa
     6121 ccagctcttt acggtaggcg gcctctactc gctggttttt aaactccagc cccggcagtt
     6181 tgatccctac cactgccagc aatccggtcc ccatcagcga ccagacgata gcggcaatca
     6241 ccagaccata cggaatgtgc ccgataatcg gcagctccgg cacatgcgcg gagagcgtta
     6301 ccagcaccgg caggaaggcg atcaacgtca ttatggcgtt gataaaactg acgcccatat
     6361 tctccagcgt tgaagcaaaa cgcatggtgt cttcctgcac acgctgtgcg gcaccttcga
     6421 tatgacgcag ttgttgccag ttcgccatgt aatattcgtt catcgccgta cgccagcgga
     6481 acacgtagtg actgacaaag aagttgttca gtacactgat caccactgcg atcagcgcaa
     6541 tccccagaaa gacgcccact tcgcggtaaa attgttcgat agtgacttta tgcggcgaac
     6601 ttagcgcggt ttgaatcaga tcatagaacg gcgcatacca ggcgttgacg gcgaccccga
     6661 cttccaccaa aaaccaagtg acgaagataa tcagcgcagt accgagtatt gaccagtatt
     6721 gccaacggtg cgggctgtag ataaaccaga aaaatgcaaa aagtcctacg caaacaatgt
     6781 agtaagcgta aaaaatcagg aaatccaacg accagaaacg cgcggcgcta atcgggatct
     6841 gcccggaagc gccggtgata tgtgccaccc agtcaccccc accggcttgc cagaagataa
     6901 cggcaatcaa tgcccaaaca aaggccgaga gaaaaaacgt tcccggcttt gggaaaaaag
     6961 acttaaacat atgcactcct gctaacttct tatcgtttta acaactgctg tgtattaacc
     7021 gcatgaccgc tacaaagcgt cagccaaatg aaaccgccca ttgtgaccca tagcaaggcg
     7081 cttagttcgc cagcctgacc ataagaattg tttcgacgga tttcacaaac caaagattgt
     7141 ttacataagc ggattaacac gcgcaggatc acaaatgcag gcattggtga gcaggaaaaa
     7201 gatcgtgttt acgcgccgtt acgtttttaa cactattatt gcactaattc gccctttgca
     7261 atccatcaat gagtagtata aatacgctca gttaccttca ttcaatctat ggacaccacc
     7321 gttgaaacgt agtctgcttt tttctgccgt gctgtgtgcg gcgtcattga cctccgtcca
     7381 tgcggcgcag ccgatcactg aaccggagtt tgcctctgat attgtcgatc gttatgccga
     7441 tcatattttt tacggcagcg gtgccacggg gatggcgctg gttgttatcg acggtaacca
     7501 gcgcgtcttt cgtagttatg gcgaaacgcg acctggtaat aacgttcgcc cgcagctgga
     7561 ttcggtcgtg cgtattgctt ccctcaccaa actaatgacc agtgaaatgc tggtgaaatt
     7621 gctcgaccag gggaccgtaa agttaaacga tccgctaagt aaatatgctc cgccaggcgc
     7681 acgcgtgccg acctacaatg ggacgcccat taccctagtg aatctggcaa cccataccag
     7741 cgccctgccc cgtgaacagc ccggtggcgc ggcacatcgt ccggtgtttg tctggccaac
     7801 gcgcgagcaa cgctggaaat acctttctac ggcgaagctg aaagccgcgc caggcagtca
     7861 ggcggcgtac tctaaccttg cgttcgacct gctggcagat gcactggcga atgcgtccgg
     7921 caagccttat acccagttgt ttgaagagca gattacccat ccattgggga tgaaagacac
     7981 cacctacacc ccctcaccgg atcagtgccg ccgtttaatg gttgctgagc gcggtgccag
     8041 tccgtgcaat aacacgctgg cggcaattgg cagcggcggc gtctattcca cgcctggcga
     8101 tatgatgcgc tggatgcagc agtatttgtc atctgatttt tatcagcgca gtaaccaggc
     8161 agatcgcatg cagacgttga tttatcagcg cgcgcagttt acgaaagtga ttggcatgga
     8221 tgtccccggc aaagcggatg cgcttggttt aggctgggta tatatggccc cgaaagaggg
     8281 tcgtccgagg attattcaga agacaggcgg tggtggcggt ttcattacct atatggcgat
     8341 gatcccgcag aagaatattg gcgcgtttgt ggtggtcact cgttcgccgt tgacgcgctt
     8401 taaaaatatg agcgatggca tcaatgatct ggtgaccgag ctaagcggga ataaaccgtt
     8461 ggttatcccc gcatcctgat taatactctg aaggcaaacg gttgatagcg accagtttgc
     8521 ctttattcat ttcgatatag ccgcctttac gcaaagcagc gagaacttct gcgacgacag
     8581 aacgcgatat gcgtgtacgt tgatggatat aattcatcac accaatacgt gatcgcaatt
     8641 cttcattcca gtcaatcatt gataataatg ttgcgcgggt ttgtttggct ggtagatgat
     8701 atcccgtaca atttccttct gatattaatt tgtatggtat atcgtttttc attaaaccat
     8761 cagccagccc cataatataa ggagcctggg taataccgat aagtacgttt tcttctctac
     8821 gcagagagat aactccttct aaaataacga aagtatcttc gttattgaca tcactggaaa
     8881 atataacttg cttttcatta ttaaactcga agcgcgtacc gtatctggac aaacatttat
     8941 cgagcttacc aaattcctga agaggtttaa ctacagataa catttgcgcg tcctttgcag
     9001 taatgcccgt caaatccttg acgggcatta tttagattaa attaccagta tttcttcgga
     9061 gtgaagaata ttaccaagta tatttaacac ccacgttcgc ggaccagtct tgatctacgt
     9121 caccaccacc gaggtagtta gcatcggtat aggcgctgaa gttcttggtg aagctgaact
     9181 gagtacccag accaacacgt accgcagacc cttcagtacc gttatcgatg gaatcaccgt
     9241 tcacatcgtt atcgttgtta gagtcgtcgt agacgtaagc cagtttgaag tacggagtca
     9301 gagcctgatc ttcgctgtag gtgaaggtat aacctgcatc tacacccagt tcataacgca
     9361 tgctgtcgta aggctgaccg tcaactttca tgtcgttgct cagctggtag tcatcaccag
     9421 actggaacag accagaaatg ctgccgtaag gagtcacgta accagcatca cccagtttga
     9481 agtcgtaacc ggttttcaaa ccgaagcccc aagcgtcgga gttggtgcta ccgtcaacgt
     9541 aagtaccgtt gctcatggtt gcagacaggt cgttgttgaa gtgagagtag ctcaagc
//
LOCUS       DAJTQY010000126         9493 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.126, whole genome shotgun sequence.
ACCESSION   DAJTQY010000126 DAJTQY010000000
VERSION     DAJTQY010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9493
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.126"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(<1..190)
                     /locus_tag="OO349_003471"
     CDS             complement(<1..190)
                     /locus_tag="OO349_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HCR5868414.1"
                     /translation="MNMMRIIYIGLSGVGMMFSSMASGHDAGGLQSPTCGVVCDPYIC
                     VNSDGISPELTRKYLGEKA"
     gene            710..1405
                     /gene="minC"
                     /locus_tag="OO349_003472"
     CDS             710..1405
                     /gene="minC"
                     /locus_tag="OO349_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HCR5868415.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALKDKIAQAPTFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTMPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            1429..2241
                     /gene="minD"
                     /locus_tag="OO349_003473"
     CDS             1429..2241
                     /gene="minD"
                     /locus_tag="OO349_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HCR5868416.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            2245..2511
                     /gene="minE"
                     /locus_tag="OO349_003474"
     CDS             2245..2511
                     /gene="minE"
                     /locus_tag="OO349_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HCR5868417.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(2883..3227)
                     /locus_tag="OO349_003475"
                     /pseudo
     CDS             complement(2883..3227)
                     /locus_tag="OO349_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(3262..3381)
                     /locus_tag="OO349_003476"
     CDS             complement(3262..3381)
                     /locus_tag="OO349_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCR5868418.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(3342..3527)
                     /locus_tag="OO349_003477"
     CDS             complement(3342..3527)
                     /locus_tag="OO349_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5868419.1"
                     /translation="MSRRYSSATITLVILFFRRPHCLKISVRQYYLKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            3628..3801
                     /locus_tag="OO349_003478"
     CDS             3628..3801
                     /locus_tag="OO349_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5868420.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            3803..4147
                     /locus_tag="OO349_003479"
     CDS             3803..4147
                     /locus_tag="OO349_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HCR5868421.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            4151..4486
                     /locus_tag="OO349_003480"
     CDS             4151..4486
                     /locus_tag="OO349_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HCR5868422.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(4543..6864)
                     /locus_tag="OO349_003481"
     CDS             complement(4543..6864)
                     /locus_tag="OO349_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR5868423.1"
                     /translation="MQGKNTIVTTGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSACACVSSLDEESTADTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAGDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAAIAKIYNGGTVTLKNTSAVAHQGSGIVLESSINGQEATVDILSGSSL
                     RSANEILYHKNETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDDN
                     THTYLSLSDNSTWDIKADSTVSNLTVYNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LHLRTELDDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPTVAPTPVLRP
                     EAGSYVANLAAANTLFVMRLNDRAGETRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGGDLLTGGFTDSDSWRLGVMAGYARDYNLTHSSVSDYRSKGSVRG
                     YSAGLYATWFADDISKKGAYIDSWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGVSNLGEIKLGVNGN
                     LNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(6981..7190)
                     /locus_tag="OO349_003482"
     CDS             complement(6981..7190)
                     /locus_tag="OO349_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5868424.1"
                     /translation="MKLKKLPGFSLGLIALAVGNAYAEQFIQHDEYKHAVAIKDGKVK
                     DIDNALVITTGNGSYGYPLQERVVS"
     gene            complement(7941..9464)
                     /locus_tag="OO349_003483"
     CDS             complement(7941..9464)
                     /locus_tag="OO349_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCR5868425.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECDLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVVPDSNLLLAPAIDTVNRLPILLYQSQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTGDVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIACAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLGYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVMVE"
BASE COUNT         2659 a         2203 c         2080 g         2551 t
ORIGIN      
        1 cggctttttc gccgagatat ttccttgtta actctgggga aatgccatct gagttcacac
       61 aaatatacgg gtcgcaaaca acgccgcacg tcggagattg aagtccgcca gcgtcatgtc
      121 cagacgccat tgatgagaac atcatcccca cacctgacaa tccgatataa ataatacgca
      181 tcatattcat agcacatcct gataatccaa ccgtacaaca cttatgctca tttcgtcatc
      241 aatccgtaat caaaaccgct gacctggacg agttacgatg acctgacaga agtatcagac
      301 gtcttctgcg gtttctatga tagagcaatt ctgtggcgga tattaagata acgggtatct
      361 gttctttaat gatgctgcaa aaaatggaaa gttaagaaag cctggcgtgg tgaaaaaata
      421 cacttgcctc aatactggat atttaaacag gtctggagat gctgtcacga ttagggtgcg
      481 gaaaaaggaa gcgtaaataa gaagaatcaa aagtacgacg gcaatgggtt gattgacaag
      541 ggtatttttt aagctatgaa tcagcgccat ttatcacaga atagactttt actctgaata
      601 aatgggaggg tgacttgcct caatataatc cagactataa catgccttat agtcttcgga
      661 acatcatcgc gcgctggcga tgattaatag ctaattgagt aaggccagga tgtcaaacac
      721 tccaatcgag cttaaaggca gtagcttcac tttatctgtg gttcatctgc atgaggcaga
      781 acctaaggtt atccatcagg cgctgaaaga caaaatcgct caggccccca catttttaaa
      841 acatgccccc gttgtactca acgtcagtgc actggaagac ccggtaaact ggtcagcgat
      901 gcataaggcg gtttcggcaa ccggtttgcg ggttattggc gtaagtggct gcaaagatgc
      961 gcaacttaaa gccgaaattg aaaaaatggg gctgcctatc ctgacggaag gaaaagaaaa
     1021 agcgccacgt ccagctcccg caccgcaggc tccagcgcaa aatacaatgc cggtcacaaa
     1081 aacgcgttta atagataccc cggtgcgttc cggtcagcgt atttatgctc cacaatgtga
     1141 tctgattgtt acaagccacg ttagcgctgg ggccgaattg attgccgatg ggaacattca
     1201 tgtctatggc atgatgcgcg gtcgtgcgct ggcaggggca agtggtgacc gggaaacgca
     1261 aatattttgt acgaacctga tggcggaatt ggtgtccatc gcaggtgaat actggctgag
     1321 tgatcaaatc ccagcagaat tttatggcaa agcggcgcga ctgcagttag tcgaaaacgc
     1381 tttgaccgtt caaccgttaa attgatccct ttttaacaag gaatttctat ggcacgcatt
     1441 attgttgtta cttcgggtaa agggggtgtt ggtaagacaa cctccagcgc ggccatcgcc
     1501 actggtttgg cccagaaggg aaagaaaact gtcgtgatag attttgatat cggcctgcgt
     1561 aatctcgacc tgattatggg ttgcgaacgc cgggtcgttt acgatttcgt caacgtcatt
     1621 cagggcgatg caacgctaaa tcaggcgtta attaaagata agcgtactga aaatctctat
     1681 attcttccgg catcgcaaac acgcgataaa gatgccctca cccgtgaagg ggtcgccaaa
     1741 gttcttgatg atctgaaagc gatggatttt gaatttatcg tttgtgactc cccggcaggg
     1801 attgaaaccg gtgcgttaat ggcactctat tttgcagacg aagccattat taccaccaac
     1861 ccggaagtct cctcagtacg cgactctgac cgtattttag gcattctggc gtcaaaatca
     1921 cgccgcgcag aaaatggcga agagcctatt aaagagcacc tgctgttaac gcgctataac
     1981 ccaggccgcg taagcagagg tgacatgctg agcatggaag atgtgctgga gatcctgcgc
     2041 atcaaactcg tcggcgtgat cccagaggat caatcagtat tgcgcgcctc taaccagggt
     2101 gaaccggtca ttctcgacat taacgccgat gcgggtaaag cctacgcaga taccgtagaa
     2161 cgtctgttgg gagaagaacg tcctttccgc ttcattgaag aagagaagaa aggcttcctc
     2221 aaacgcttgt tcggaggata agttatggca ttactcgatt tctttctctc gcggaagaag
     2281 aacacagcca acattgcaaa agaacggctg cagattattg ttgctgaacg ccgtcgcagc
     2341 gatgcagaac cgcattatct gccgcagttg cgtaaagata ttcttgaggt catttgtaaa
     2401 tacgtacaaa ttgatcctga gatggtaacc gtacagcttg agcaaaaaga tggcgatatt
     2461 tctattcttg agctgaacgt gaccttaccg gaagcagaag agctgaaata agcccgctgt
     2521 aaaaacgcat ttatcttcaa ggcagagtca tttctgcctt gagtttttca tccctctcat
     2581 ccacgttgtg gtaaagcggc gagtattctt gctgatactc ctcattgcta tttccacctc
     2641 cccctttaca ccttaaggct gttcccacgc acagaacaaa atgtttatgc ttggttgagt
     2701 aaataaccat gttattagtg tgtatatact gggagatgat aaccccgcaa tttcattatt
     2761 cattgttctc ccataggatg aggtttcccg ccgtaactgg cgggcttttt ttgccccaga
     2821 attttctctt tcaacatcct gtaaacgaaa actgcgccga agcgcagttt aatcgttgat
     2881 ggtcagaacg accagttcac accagccacc gcgttccacg gggactccac accggcacca
     2941 tggctatacc ccaccccgaa atgcccgctt aacgtactgc tgaatgaggc tttaatacct
     3001 gcctggtata ttccacgtct gcccgacaaa tcattgacaa aattaccgtc actattcact
     3061 ttcacccgat tatcatcgac aaattctttg cgcacagccg ccttcaggca gggttcaatt
     3121 tccataccgt tccccagacg catgttgtaa ctcagcgttg cacccagttc acgatatata
     3181 ctgcgggtat cgactgattt cgattccatg ccattggata aatgatagta gcgtctaagt
     3241 gacctaagct ctgctgcaac atcagcccgc aggcaccaga ccaggggatt catcctgaag
     3301 agacagcgca ggtgtattgt gttcaccgct caccaaagac atcatgatga aatgatgata
     3361 tcccgcataa gaaagaggca ttttttacac gcagtgcgct gaagtgtagt tggataaaaa
     3421 atcactcttt tcagataata ctgtcgtacg cttatcttta gacaatgagg ccgtcggaaa
     3481 aataaaatga ctaatgtaat cgttgctgat gaatatcttc tgctcacata aaaatcacac
     3541 aataatattg ataaatcaga ttatcttacc tttacaacat tgcgttaaca gtcaatgaca
     3601 cttcttgaat aaaaatattg tcaatcaatg agttattcct catttaagat tattctcatt
     3661 cacgtaaaaa acataattcc tatcatcaca gccacgctca ttttgaatta tctgaataat
     3721 agcgagcgtt ctttagttaa gcagatctta attgaagacg aaattattgt gtgtgcaact
     3781 tacttaatac cggacatcta agttgaagaa aaaaatactt gccttcgggc ttatctcagc
     3841 attgttctgc tccacgccag cgatggcgga tatgaaccgc accacgaaag gtgcattgct
     3901 aggcgcaggc gttggtttac ttactggcaa tggtgttaat ggcgtactga aaggtgcagc
     3961 tgttggcgct ggtgttggtg cagtaacaga aaaaggccgc gacggtaaaa atgcgcgtaa
     4021 aggtgcaaaa gtgggtgccg ccgttggtgc tgtcactggc gttctgaccg gcaatggcct
     4081 cgaaggggcg attaaaggtg ccgtgattgg cggtacgggt ggtgcaattc tgggtaaaat
     4141 gaaataagga atgatgatga aaaaatttgc tttactggcg ggcctgtttg ttttcgcacc
     4201 aatgacctgg gcgcaggact acaatattaa aaacggtttg ccgtcagaaa cctatattac
     4261 ttgcgcagaa gccaacgaaa tggcaaaaac tgacagcgca caggttgcag aaattgttgc
     4321 ggtaatgggt aatgccagcg ttgccagccg tgatttaaaa attgagcaat ctccggaact
     4381 gagcgccaaa gtggtagaaa aactgaatca ggtttgcgct aaagacccgc aaatgctgtt
     4441 gatcacggca attgatgaca ccatgcgtgc tatcggcaaa aaataatatt tcatatgtac
     4501 cgcgtcgatg aatacgacgc ggttttttag ctaacggcgg gatcagaact tatatttcag
     4561 gcccaccatc actgcggtgt cattgtagcc attatcaccc agctgcacgc ccacattccc
     4621 ccagacgctg gccgctggat tcaggttgcc atttacgcca agtttaattt cccccaaatt
     4681 actcacccca tcttgttcga ctttcacacc gttcattgag acagcaaaat ctttactgtt
     4741 atggatccag ttcatttcaa caaaaggttc aaagtcgtca ccgtgcggac cgctgttttt
     4801 ctcctgagta cgaataaagg tgcggaagcc gagtcgggtc tggatgttgt tatttgcgtc
     4861 attctcaata cgtgagccat tggcttccgt gtgcgcatta tgatcgacgc ccatccagat
     4921 agcctgtgcc tgtggctgga agatccacgt atatttcgcc gcttccagac caaaggattt
     4981 attcagggca aaaccgtaac ccgcttccag cgagacggtt gcacctttcg cgctatagga
     5041 ttcataggct aattcatccc ctttcacgga gtttttaaac cagctatatt gcgcccagga
     5101 gtcaatgtat gcgccttttt tactgatgtc atcggcaaac caagtggcat acagccctgc
     5161 gctatagcct ctgacactcc ctttcgaacg ataatccgac acgctggaat gagttaagtt
     5221 gtagtcgcgg gcataaccag ccatcactcc caaacgccaa ctgtcactat cggtaaaacc
     5281 accggttaac agatcgcccc ctaactgcga gacgtagcga tgcgaggtcg ttctcaactg
     5341 tccgttgctg tcacgccagg cattatgccc gccaatttga cgtagccaaa gtcggcttga
     5401 acgctcctgt tcagttacag gatcgatgta gcgcgtttca cccgcacggt cgttcagacg
     5461 cataacaaaa agagtgttag cggctgccag gttggcgacg taacttccag cttcggggcg
     5521 caggacgggg gtaggcgcaa cagtaggcgc tgaacttcct ccggagtttg tttcaccgcc
     5581 gctcgttgcc tggaagttag tcagatacca gtttttattg gtcgcttcgg tattacctcg
     5641 ggttaatgaa tattcgtagg caccggcgaa aatcctcgaa tccttaataa attccccatt
     5701 tgattccccc tcaacgctga taatctctat cccatttaac gtgtaagctc cgctgcctcc
     5761 tgcattggta actttaactc gggttgttcc agaggtattt ccattaataa cgactttatc
     5821 cgtagctgaa ttatcatcat ccaattcagt tctgagatgc aatacgccat tattaccaac
     5881 gtaattttca gttattgtta atcttgttgg ttcgacatcc cgtccatctg cccgggaaat
     5941 atagaccgta ctattataaa ccgtgaggtt gctcaccgtt gagtcagctt taatatccca
     6001 ggtactatta tctgaaagcg acagataggt atgagtgtta tcatctgttg aaatattagc
     6061 tgaacccgtt atttttgaat tagttgcatc gacggtcaaa tgacctttaa tattattgat
     6121 aaaaacatct gcagccgatg atacctcact atccgtaatg gtcacgttac tcgtctcatt
     6181 tttatggtag aggatttcat ttgctgaccg tagtgagcta ccagataaaa tatctaccgt
     6241 tgcttcctga ccatttatgg aagactccag cacaattcca gacccctgat gcgcgactgc
     6301 agatgtattt ttcagcgtca ccgtgccgcc attataaatt ttagcaatcg cggcgtcagg
     6361 acccgttgta taaatttctc cctggttaaa tgaaactgtc ccctggcggg cagcgatggc
     6421 atgagcattg tccccatgcg tggtgatatc aacatcggtt aatgtaacgc tggcattggt
     6481 ataatcaagc gtgtcaccag ccttgacgac agtgccatta gcgtacgcgg cataactttc
     6541 tgttccatgg gttgtgatct ccccttttgc catatccagt tttgctatgg cattattttc
     6601 attacttcct gtatcggcag tactttcttc atcaagagaa gatacacacg cacacgcgct
     6661 tccgggagat gcacacgcaa gaacgccgac tgcgtgatta cccgcagtaa caataaatgt
     6721 atcatctccg tcagaaaaag agggggtaac cgaaccgtca gagttgactc ttattatggt
     6781 gtcagtattc aggtttccac tcgtttgact tagcaaacca attgagtaat cccccgttgt
     6841 gacaatcgtg tttttaccct gcaaaataac agcaccgcca aattcactta cgattgcgct
     6901 agctgttaat gctgggctta gaggactatt gtcagaagga tataaaccgc ccgttgttac
     6961 aatgacgcca ttgttaatgg ttaacttact accctctcct gcaacggata cccgtaactt
     7021 ccattacccg ttgttataac tagcgcatta tcaatatctt ttacttttcc atcttttatt
     7081 gccacagcat gtttatattc atcgtgctga ataaactgtt cagcatatgc attacccacc
     7141 gccagagcaa taagtcccaa actaaacccg gggagttttt tcagtttcat agattaacta
     7201 tccattcatt ggattaacaa acattcctgg tcaaataacc tatgagctca gattaccaaa
     7261 acggagggta aagacaaaaa caaaagcaca aaaaatttaa atagataaac agcgacaata
     7321 tataaatgat aaataagtcg gcattatcat ttttcacctt attttcatga cattgatcac
     7381 tttggggtga ctattttgtt taacaaataa ctcttcgaca tcaaaattat taatagttgc
     7441 catggtaatt atttcggtga tatataaaaa caactgtgat aacaccaagg ttgacaacta
     7501 tttgcgcaat aattcctttc cttcaatgat ccaggcgttc actgccagca acccattcag
     7561 ccgcatactg acagtgagaa actgaaagat tattgctgat ccctgtggct aacgagtctt
     7621 tctcttaaat cgattaacca gattgatcgc cagccaaaca agggcattaa gcaaatttat
     7681 cgtcacaagc acttctccca gcccggtact gggttttgca ataaggatga ccaggagggt
     7741 gatcagcata aatgcaatgg aaaagatgat atagagagtg aaataatcca gattattact
     7801 attattcatg ctactaatgg caatcctgtc aaaactttca aacaattata gcaaacaaaa
     7861 taatgaacaa tagtaacaag aggcaacgag ctaattagct gtctataatt acaccgcact
     7921 atggccttgc tgatcaattt tcactcaacc ataaccttca ccttcggttt agaaaggata
     7981 attttgacca ggtcgatgta tgtaacaggt ttatagaaat aataaccctg ctgaaatgtg
     8041 atattatttt ggttcagata gccaagttgt tctttcgttt cgacaccttc agcgacgata
     8101 ctgatcgaaa gtttacgcgc cagatccaat acacaatcca gaattcgggt tgattctggg
     8161 tctgcgttta cgcggccaac gaaactgtga tcaattttga tataatcaat atgcaggtca
     8221 tgaagataag aaagccctga gtagccagta ccgaaatcat ccagcgcgat gacaaaacca
     8281 ttttcatgca gaatgttcaa ccgctgaacc agactttcat caacattcaa tggctcacgc
     8341 tcggtgactt ccagaacaag gtttaaatcg cggcgggtaa aactgtcacg gaaatttaaa
     8401 cactcgtcga caaacgtcgg cgaaataata tgcgaagcgc taaaattaat tccaatatga
     8461 aaaccttccg gcagtttact cgcgatagcg ttcatctgtc tggcaacctg attcatcagg
     8521 ctttgtgtaa gcggaacgat taatccggat ttttcagcaa gtggaataaa tgccgcgggt
     8581 gaaatatatc caccgtgagg ttgtttccag cgggctaaca cctcaactcc ccgcaatgtc
     8641 ccttcccgac catttaccac aggttggtaa aaagggacga tctccccatt atttattgcc
     8701 cgtcgcagga tctcttcagg cgttgcgctt ttattgaaat atctatcaag caggaaggca
     8761 gcggcgcagg cgattaacag aataaatatt aaaatcccaa agcctttatc gataagtctg
     8821 tagaagctaa agagtggcgg cgggttaaag ataatagtga aatgatattt tgtggagtga
     8881 accttctcgg tatagggata atgtccatta agcgtcatca catcacccgt tggtccaata
     8941 atgtcatccg ccacgcgtaa tacatacctt acccctttca agggtacatt aagcgccccg
     9001 cgaatatgtt gatcacttat cgtaaccaaa atgcgcgtat ctgcaaattg gctctgatag
     9061 agcaggatag gtaatctatt aacggtgtcg attgccggag ccaacagtaa attactgtcc
     9121 gggacaacag gaatacgaga cagcaggacc cgattcccag gcagggatgt acaccagact
     9181 attccctgtt tgagaatgat gattgtgcgc aggtgaggtt tcagagctgc ttcagtgcca
     9241 agctgatatt gctcctcgag gtcgcattcc ttcccggcaa tgttaaccgc tgtttgagtc
     9301 gcctgtgacg cttcttcaag tatatgattg atattgttgg cggcatatct tgctcccgcc
     9361 aggtactctg cttttgcgga ataccagagc tggatgttca aaatagcgac accggtaata
     9421 aacaagcata tcgcaacgag gatgggtatg agtgtattgc gcatttgctt gtcccggcca
     9481 aagggtttaa tac
//
LOCUS       DAJTQY010000127         9398 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.127, whole genome shotgun sequence.
ACCESSION   DAJTQY010000127 DAJTQY010000000
VERSION     DAJTQY010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9398
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.127"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(383..1360)
                     /gene="epmA"
                     /locus_tag="OO349_003484"
     CDS             complement(383..1360)
                     /gene="epmA"
                     /locus_tag="OO349_003484"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HCR5868426.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1685..3493
                     /gene="frdA"
                     /locus_tag="OO349_003485"
     CDS             1685..3493
                     /gene="frdA"
                     /locus_tag="OO349_003485"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HCR5868427.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAINMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTIPTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LSHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANNKEKANG"
     gene            3486..4220
                     /gene="frdB"
                     /locus_tag="OO349_003486"
     CDS             3486..4220
                     /gene="frdB"
                     /locus_tag="OO349_003486"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HCR5868428.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4231..4626
                     /gene="frdC"
                     /locus_tag="OO349_003487"
     CDS             4231..4626
                     /gene="frdC"
                     /locus_tag="OO349_003487"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HCR5868429.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4637..4996
                     /gene="frdD"
                     /locus_tag="OO349_003488"
     CDS             4637..4996
                     /gene="frdD"
                     /locus_tag="OO349_003488"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCR5868430.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            5059..6192
                     /locus_tag="OO349_003489"
     CDS             5059..6192
                     /locus_tag="OO349_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCR5868431.1"
                     /translation="MFKTTLCALLITASYSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            6281..6814
                     /gene="blc"
                     /locus_tag="OO349_003490"
     CDS             6281..6814
                     /gene="blc"
                     /locus_tag="OO349_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCR5868432.1"
                     /translation="MRLLPLVAAATAAFLVVACSFPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6811..7128)
                     /gene="sugE"
                     /locus_tag="OO349_003491"
     CDS             complement(6811..7128)
                     /gene="sugE"
                     /locus_tag="OO349_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HCR5868433.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7304..7450)
                     /gene="ecnB"
                     /locus_tag="OO349_003492"
     CDS             complement(7304..7450)
                     /gene="ecnB"
                     /locus_tag="OO349_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HCR5868434.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7561..7686)
                     /gene="ecnA"
                     /locus_tag="OO349_003493"
     CDS             complement(7561..7686)
                     /gene="ecnA"
                     /locus_tag="OO349_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HCR5868435.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            complement(7738..8304)
                     /gene="efp"
                     /locus_tag="OO349_003494"
     CDS             complement(7738..8304)
                     /gene="efp"
                     /locus_tag="OO349_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCR5868436.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8346..9374
                     /gene="epmB"
                     /locus_tag="OO349_003495"
     CDS             8346..9374
                     /gene="epmB"
                     /locus_tag="OO349_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HCR5868437.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKEHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         2377 a         2422 c         2416 g         2183 t
ORIGIN      
        1 aaggcgaatg gaataaagaa taacaatgca gaaaatatat aaaagggaat cgcactataa
       61 cccattaagt aataagccga tgggctattg gcaaatccaa atacggaagt aaagatcatc
      121 aatatgagtc ctatcagact catctttttt atcgtgtgag gcatgaaacc atccttcatt
      181 tcagcgcgtg tataacacgt gcccactgaa ctgcactgcg caacattgag caggcagcgt
      241 aaaaaccaat acagaattga tacttggatg atatcaaata tccagtctta aattgtggcc
      301 tttaacggaa aaatgaaaac atatgctgca gaatggcgct tatcacgcca ttcttcgctg
      361 ttaattcagt aatttttcag aattatgccc ggtcaacgct aaaggcgatg acttcagcca
      421 gtgtctccgc gcccagcgcc aacatcacca gacgatcaac acctaatgcc acgccggaac
      481 agtcaggcat accaactttc aaggcttcaa tcagattctg gtcaatgggg tgctgcggca
      541 aaccgcgcgc cgcgcgctta cggttatctt gttcaaagcg ttgttgctgc tcacgggcat
      601 ccgttaattc atggaaacca ttcgccagct caataccttt ataataaacc tcaaagcgtt
      661 cagcgacccg atgatcttcg gtactgattt gcgccagtga tgcctggctg gctggaaagt
      721 ggtacacaaa ggtcggtttt tctttaccaa tatttggctc tacgccaaag gtaaacagca
      781 attgtagcaa cgtgtcgcgg tcttcttcgg tatcagcaac attgctcaaa tccagtttcg
      841 ctgcgacttc ccgcagttgc gttttgtcgg cagagagcgg gtcaatttcc agataacgca
      901 agaaagcttg ttgataagaa aggctttctg ctgccgggca gtccagcacc tgttgtaaga
      961 gatcgtccac ctcgttcatc aaccggtaca tatcatagtg cggtcgatac cactccagca
     1021 tagtgaactc agggttgtga taacgcccca tctcttcatt acggaagctg cggcacagct
     1081 ggaataccgg cccacaacca gcaaccagca ggcgtttcat atggtattcc gggctggtca
     1141 ttaaccagag attcatcccc tgcgaatgcc cggggccaac gaaacgtgtc tcaaacggga
     1201 ccaaatgaat atcggttacc gtcgcctggc tcatacaagg cgtctccacc tccagcactc
     1261 cacgatcggc aaagaaacga cggatctccg ccataatcgc cgcgcgtttt aataagttag
     1321 gaatggatgc gctcggctgc caggatgccg tttcgctcat agttaaatct ccagtttttg
     1381 acaagggcac gaagtctact cgcaacgcga cggcgagaca aattttacgc aggaatcaaa
     1441 cagcggtggg cagtgactaa aaaaagcacg atctgatggt ttagtaatta aattaatcat
     1501 cttcagtgat aatttagccc tcttgcgcac taaaaaaatc gatctcgtca aatttcagac
     1561 ttatccatca gactatactg ttgtacctat aaaggagcag tggaatagcg ttcgcagacc
     1621 gtaactttca ggcacttacc ctgaagtacg gggctgtggg ataaaaacaa tctggaggaa
     1681 tgtcgtgcaa acctttcaag ccgatcttgc cattgtaggc gccggtggcg cgggattacg
     1741 tgctgcaatt gctgccgcgc aggcaaatcc aaatgcaaaa atcgcactaa tctcaaaagt
     1801 atacccgatg cgtagccata ccgttgctgc agaagggggc tccgccgctg tcgcgcagga
     1861 tcatgacagc ttcgaatatc actttcacga tacagtagcg ggtggcgact ggttgtgtga
     1921 gcaggatgtc gtggattatt tcgtccacca ctgcccaacc gaaatgaccc aactggaact
     1981 gtggggatgc ccatggagcc gtcgcccgga tggtagcgtc aacgtacgtc gcttcggcgg
     2041 catgaaaatc gagcgcacct ggttcgccgc cgataagacc ggcttccata tgctgcacac
     2101 gctgttccag acctctctgc aattcccgca gatccagcgt tttgacgaac atttcgtgct
     2161 ggatattctg gttgatgatg gtcatgttcg cggcctggta gcaataaaca tgatggaagg
     2221 cacgttggtg cagatccgtg ctaacgcggt cgttatggct accggcggtg cgggtcgcgt
     2281 ttatcgttac aacaccaacg gcggcatcgt taccggtgac ggtatgggta tggcgctaag
     2341 ccacggcgtt ccgctgcgtg acatggaatt cgttcagtat cacccaaccg gtctgccagg
     2401 ttccggtatc ctgatgaccg aaggctgccg tggtgaaggc ggtattctgg tcaacaaaaa
     2461 tggctaccgt tatctgcaag attacggcat gggcccggaa actccgctgg gcgagccgaa
     2521 aaacaaatat atggaactgg gtccacgcga caaagtttct caggccttct ggcacgaatg
     2581 gcgtaaaggc aacaccatcc ccacgccgcg tggtgatgtg gtttatctcg acctgcgtca
     2641 cctcggcgag aaaaaactgc atgaacgtct gccgttcatc tgcgaactgg cgaaagcgta
     2701 cgttggcgtc gatccggtta aagaaccgat tccggtacgt ccgactgcac actacaccat
     2761 gggcggtatc gaaaccgatc agaactgtga aacccgcatt aaaggtctgt tcgccgtggg
     2821 tgaatgttcc tctgttggtc tgcacggtgc aaaccgtctg ggctccaact ccctggcgga
     2881 actggtggtc ttcggccgtc tggccggtga acaagcgaca gagcgtgcag caactgccgg
     2941 taatggcaac gaagcggcaa ttgaagcgca ggcagctggc gttgaacaac gtctgaaaga
     3001 tctagttaac caggatggcg gcgaaaactg ggcgaagatc cgcgacgaaa tgggcctggc
     3061 aatggaagaa ggttgcggta tctaccgtac gccggaactg atgcagaaaa ccatcgacaa
     3121 gctggcagag ctgcaggaac gcttcaagcg cgtgcgcatc accgacacct ccagcgtgtt
     3181 caacaccgac ctgctctaca ccattgaact gagccacggt ctgaacgttg ctgaatgtat
     3241 ggcgcactcc gcaatggcac gtaaagagtc ccgcggcgca caccagcgtc tggacgaagg
     3301 ttgcaccgag cgtgacgacg tcaactttct caaacacacc ctcgccttcc gcgatgctga
     3361 tggcacgact cgcctggagt acagcgacgt gaagattact acgctgccgc cagctaaacg
     3421 cgtttacggt ggcgaagcgg atgcagccga taaggcggaa gcagccaata ataaggagaa
     3481 ggcgaatggc tgagatgaaa aacctgaaaa ttgaggtggt gcgctataac ccggaagtcg
     3541 ataccgcacc gcatagcgca ttctatgaag tgccttatga cgcaactacc tcattactgg
     3601 atgcgctggg ctacatcaaa gacaacctgg caccggacct gagttaccgc tggtcctgcc
     3661 gtatggcgat ttgtggttcc tgcggcatga tggttaacaa cgtgccaaaa ctggcatgta
     3721 aaaccttcct gcgtgattac accgacggta tgaaggttga agcgttagct aacttcccga
     3781 ttgaacgcga tctggtggtc gatatgaccc acttcatcga aagtctggaa gcgatcaaac
     3841 cgtacatcat cggcaactcc cgcaccgcgg atcagggtac taacatccag accccggcgc
     3901 agatggcaaa gtatcaccag ttctccggtt gcatcaactg tggtctgtgc tacgccgcgt
     3961 gcccgcagtt tggcctgaac ccagagttca tcggtccggc tgccattacg ctggcgcatc
     4021 gttataacga agatagccgc gaccacggta agaaggagcg tatggcgcag ttgaacagcc
     4081 agaacggtgt atggagctgt actttcgtgg gctattgctc cgaagtctgc ccgaaacacg
     4141 tcgatccggc tgcggccatt cagcagggca aagtagaaag ttcgaaagac tttcttatcg
     4201 cgaccctgaa accacgctaa ggagtgcaac atgacgacta aacgtaaacc gtatgtacgg
     4261 ccaatgacgt ccacctggtg gaaaaaattg ccgttttatc gcttttacat gctgcgcgaa
     4321 ggcacggcgg ttccggctgt gtggttcagc attgaactga ttttcgggct gtttgccctg
     4381 aaaaatggcc cggaagcctg ggcgggattc gtcgactttt tacaaaaccc ggttatcgtg
     4441 atcattaacc tgatcactct gacggcagct ctgctgcaca ccaaaacctg gtttgaactg
     4501 gcaccgaaag cggccaatat cattgtaaaa gacgaaaaaa tgggaccaga gccaattatc
     4561 aaaagtctct gggcggtaac tgtggttgcc accatcgtaa tcctgtttgt tgccctgtac
     4621 tggtaaggag cctgagatga ttaatccaaa tccaaagcgt tctgacgaac cggtattctg
     4681 gggcctcttc ggggccggtg gtatgtggag cgcaattatt gcgccggtga tgatcctgct
     4741 ggtgggtatt ctgctgccac tggggctgtt tccgggtgat gcgctgagct acgagcgcgt
     4801 tctggcgttc gcgcagagct tcattggtcg cgtattcctg ttcctgatga tcgttctgcc
     4861 gctgtggtgt ggtttacacc gtatgcacca cgcgatgcac gatctgaaaa tccacgtgcc
     4921 tgcgggcaaa tgggttttct acggtctggc tgctatcctg acagttgtca cgctgattgg
     4981 tgtcgttaca atctaacgca tcgccaatgt aaatccggcc cgcctatggc gggccgtttt
     5041 gtatggaaac cagaccttat gttcaaaacg acgctctgcg ccttattaat taccgcctct
     5101 tactccacat ttgctgcccc tcaacaaatc aacgatattg tgcatcgcac aattaccccg
     5161 cttatagagc aacaaaagat ccccggtatg gcggtggcgg taatttatca gggtaaacct
     5221 tattacttta cctggggcta tgcggacatc gccaaaaagc agcccgtcac acagcaaacg
     5281 ttgtttgagt taggttcggt cagcaaaaca tttacgggcg tgcttggtgg cgacgctatt
     5341 gctcgagggg aaatcaagtt aagcgatccc gcaacaaaat actggcctga acttaccgct
     5401 aaacagtgga atgggatcac actattacat cttgcgacct acaccgctgg cggcctgcca
     5461 ttgcaggtgc cggatgaagt gaaatcctca agcgacttgc tgcgcttcta tcaaaactgg
     5521 cagcctgcat gggctccagg aacacaacgt ctgtatgcca actccagtat cggtttgttc
     5581 ggcgcactgg ctgtgaagcc gtctggtttg agttttgagc aggcgatgca aactcgtgtc
     5641 ttccagccac tcaaactcaa ccatacgtgg attaatgtac ctcccccaga agaaaagaat
     5701 tacgcctggg gatatcgcga aggtaaggca gtgcatgttt cgccaggggc gttagatgct
     5761 gaagcttatg gtgtgaagtc gaccattgaa gatatggccc gctgggtgca aagcaattta
     5821 cccccccttg atatcaatga gaaaacgctt caacaaggga tacaactggc acaatctcgc
     5881 tactggcaaa ccggcgatat gtatcagggc ctgggctggg aaatgctgga ctggccggtc
     5941 aatcctgaca gcatcattaa cggcagtgac aataaaattg cactggcagc acgcccggta
     6001 aaagcgatta agcccccaac tcctgcagta cgcgcatcat gggtacataa aacgggggcg
     6061 accggcggat ttggtagcta tgtcgcgttt attccagaaa aagagctggg tatcgtgatg
     6121 ctggctaaca aaaactatcc caatccagcg agagtcgccg ccgcctgtca gattctcaac
     6181 gctctacagt aaaatttcat cgggtccgaa ttttcggacc ttttctgcgc ttttccttgc
     6241 tgtcatctac acttagaaaa aaaccagtaa ggaaacattt atgcgcctgc tccctctcgt
     6301 tgccgcagcg acagctgcat ttctggtcgt tgcctgtagt tttcctacgc cgccgcgtgg
     6361 cgtgaccgta gtgaataatt tcgacgccaa acgttatctc ggtacctggt atgagattgc
     6421 ccgttttgat caccgttttg aacgtggact ggaaaaagtc accgcaacat acagcctgcg
     6481 tgatgacggc ggcctgaatg tcattaataa aggctataac cctgacagag gaatgtggca
     6541 gcagagtgaa gggaaagcgt actttaccgg cgcaccaact cgcgctgcgc tgaaagtgtc
     6601 attctttggt cctttctatg gcggttataa cgttattgca ctcgatcggg aataccgcca
     6661 tgcgctggtt tgcggcccgg accgcgacta cctgtggata ctctcccgca cgccaaccat
     6721 ttctgacgaa gtgaaacagg agatgctggc agtcgcgacc cgggaagggt ttgatgtcag
     6781 taagtttatt tgggtacagc agcccggtag ttagtgagtg ctgagtttca gaccaataat
     6841 ccccaatacg attaacgcca gactcgccag acgcatcggg ttagcggact caccgagcag
     6901 cacaatgccg gtgatggccg cgccgactgc gccaataccc gtccacacgg cataagccgt
     6961 ccctaccggt aacgatttca tcgcccaggc aagtagcgcc atactgacta tcatcgccgt
     7021 cacggtaata acactcggcg tcaaacggct aaagccgtgg gtatatttca ggccaacggc
     7081 ccataccact tccagcagac cagcaataac taagataatc caggacatat caggctccgg
     7141 aaatttgggg ccgtccccgg tgaaaagagg cgtttgcagg tcgtcctgca aagctgatgg
     7201 ttgaacggat attttgccag gtggcagggg aatttcaact attaccttta tgacgatcgc
     7261 agaaaaaaca aacggcacga cactgctgtc gtaccgttat tgcttattgc tgcgctttcg
     7321 ttgctgcgcc agaaatcgcg ttaccgccat cagaaatgtc ttcaccaacg ccacgcgtgg
     7381 tgttgcaggc agttaatact gttgaaagca ccagaacaga aaagatcgct gcaattgtct
     7441 tcttcaccat aacgtcttcc ttttaggtaa tgttatttat gtttgcctat agcaaattaa
     7501 taatagacaa catcgccaaa aatgatggaa atctgatgat ttttcggaag agaagaaaaa
     7561 ttagctggca gcgcgagaga tggagttacc gagatgtttg atgtcttcgc cgaaaccgca
     7621 agcggtgtta cagcccgtga gcagcgtgct ggcaagcaaa acaagaacga taaggcgttt
     7681 catcatctct gacctgaata agtgatggtg cagcctgcag gccgcaccac aaccgcatta
     7741 cttcacgcga gagacgtatt caccagagcg ggtatccact ttgatgactt cgccgatttg
     7801 tacgaacagc ggaactttaa ccacagcgcc agtagacagg gtagccggtt tgccgccagt
     7861 acctgcggta tcacctttca ggcccggatc ggtatcaacg atttccagtt caacgaagtt
     7921 cggcggagta acggagatcg gctgaccatt ccacagagtt acgatacact ctgcctgatc
     7981 cagcagccat ttagcattgt cgccaattgc ttttgcatca gcagacagct gctcgaaagt
     8041 ttcgttgttc atgaagtgcc agaactcacc gtcgttgtac aggtaagtca ggttcatatc
     8101 gacaacatca gcgccttcag cggaatcagt agatttgaag gttttttcta cgcgagtacc
     8161 ggtcagcaga cgacgcagtt taacgcgagc aaatgcctgg cctttacccg gttttacgaa
     8221 ttcactcgct tcaaccgcgt aaggttcgcc gtctaacatg attttaagac cagcacgaaa
     8281 atcgttgcta tagtacgttg ccataaggcc ctctgaaatt tgttaattgg tagctaagcc
     8341 acaaaatggc gcatattgta accctaaata ccccatccag agaagattgg ttaacgcaac
     8401 ttgccgatgt tgtgaccgat cctgatgaac ttctgcgtct tttgaatata gacgcggacg
     8461 aaaaactgtt ggccggacgc agcgccaaaa agctgtttgc cctgcgtgtg ccccgctcat
     8521 ttatcgatcg catggagaaa ggcaatccgg acgatcctct tttgcgtcag gtacttacct
     8581 cgcaagatga gtttgtcgtc gcgtccggat tctccaccga cccactggaa gaacagcaca
     8641 gcgtagtgcc tggtttgttg cataaatacc acaaccgggc gcttttgctg gtcaaaggcg
     8701 gctgcgcggt aaattgccgc tattgcttcc gtcgccactt cccctatgcc gaaaatcagg
     8761 gcaacaagcg taactggcag actgcacttg agtatgttgc tgcgcatccg gaactggacg
     8821 agatgatttt ctccggcggc gatccgctga tggcgaaaga gcacgagctg gactggttgc
     8881 tcacacaact ggaagccatc ccgcatatca aacgtctgcg gattcacagc cgtctgccga
     8941 ttgtgatccc ggcacgtatc accgaggcgc tggttgaacg ctttgcccgt tctacgctgc
     9001 aaatcttgct ggtgaatcac atcaatcatg ccaatgaggt agatgaaacg ttccgtcagg
     9061 cgatggctaa attgcgccgt gtcggtgtca ccctgcttaa ccagagcgtt ctgttacgtg
     9121 gtgtgaacga taacgcacaa acgttggcaa acctgagtaa tgcgttgttc gatgccggcg
     9181 tgatgccgta ttacctgcat gtgctagata aagtacaggg cgcggcgcat tttatggtga
     9241 gtgatgacga agcacggcag attatgcgtg agttgctgac attggtgtcg ggatatctgg
     9301 tgccgaaact ggcgcgagaa attggcggcg aacccagcaa aacgccgctg gatctccagc
     9361 tacgccagca gtaactgatt aaacagggaa gttaaaca
//
LOCUS       DAJTQY010000128         9337 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.128, whole genome shotgun sequence.
ACCESSION   DAJTQY010000128 DAJTQY010000000
VERSION     DAJTQY010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9337
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.128"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            162..1697
                     /gene="gadC"
                     /locus_tag="OO349_003496"
     CDS             162..1697
                     /gene="gadC"
                     /locus_tag="OO349_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HCR5868438.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            1828..3147
                     /locus_tag="OO349_003497"
     CDS             1828..3147
                     /locus_tag="OO349_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 10 protein"
                     /protein_id="HCR5868439.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            3445..3486
                     /locus_tag="OO349_003498"
     CDS             3445..3486
                     /locus_tag="OO349_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5868440.1"
                     /translation="MVQLVDLARCVSF"
     gene            3524..4669
                     /gene="dosC"
                     /locus_tag="OO349_003499"
     CDS             3524..4669
                     /gene="dosC"
                     /locus_tag="OO349_003499"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HCR5868441.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIALAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKVLLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRILNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSFLSQKRLVKSKILGAGSSRKLAVS"
     gene            4594..5211
                     /locus_tag="OO349_003500"
                     /pseudo
     CDS             4594..5211
                     /locus_tag="OO349_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            5469..5801
                     /gene="ddpX"
                     /locus_tag="OO349_003501"
                     /pseudo
     CDS             5469..5801
                     /gene="ddpX"
                     /locus_tag="OO349_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
     gene            5815..6711
                     /locus_tag="OO349_003502"
     CDS             5815..6711
                     /locus_tag="OO349_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HCR5868442.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLMR
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTSV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            6708..7694
                     /locus_tag="OO349_003503"
     CDS             6708..7694
                     /locus_tag="OO349_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5868443.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGEDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIAGEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGGTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQHCACWYPQQEVISV"
     gene            7687..8613
                     /locus_tag="OO349_003504"
                     /pseudo
     CDS             7687..8613
                     /locus_tag="OO349_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(8669..9100)
                     /gene="osmC"
                     /locus_tag="OO349_003505"
     CDS             complement(8669..9100)
                     /gene="osmC"
                     /locus_tag="OO349_003505"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HCR5868444.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            complement(9273..>9337)
                     /locus_tag="OO349_003506"
     CDS             complement(9273..>9337)
                     /locus_tag="OO349_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5868445.1"
                     /translation="KSVELHDKVIGHYLNIKHYQ"
BASE COUNT         2284 a         2278 c         2336 g         2439 t
ORIGIN      
        1 acctgataac gtttaacggt aacggtgtcc cgaaacgaac ccgtttcggg ataatttcca
       61 aagtctgttc actggcatta gcaacggaaa atattgttct gaatacgctt cagaacaaaa
      121 caggtgcggt tccgacagga ataccgtttt agggggataa tatggctaca tcagtacaga
      181 caggtaaagc taagcagctc acattactcg gattctttgc cataacggca tcgatggtaa
      241 tggctgttta tgaataccct accttcgcaa catcgggctt ttcattagtc ttcttcctgc
      301 tattaggcgg gattttatgg tttattcccg tgggactttg tgctgcggaa atggccaccg
      361 tcgacggctg ggaagaaggt ggtgtcttcg cctgggtatc aaatactctg ggtccgagat
      421 ggggatttgc agcgatctca tttggctatc tgcaaatcgc cattggtttt attccgatgc
      481 tctatttcgt gttaggggca ctctcctaca tcctgaaatg gccagcgctg aatgaagacc
      541 ccattaccaa aactattgca gcactcatca ttctttgggc gctggcatta acgcagtttg
      601 gtggcacgaa atacacggcg cgaattgcta aagttggctt cttcgccggt atcctgttac
      661 ctgcatttat tttgatcgca ttagcggcta tttacctgca ctccggtgcc cccgttgcta
      721 tcgaaatgga ttcgaagacc ttcttccctg acttctctaa agtgggcaca ctggttgtat
      781 ttgttgcctt cattttgagt tatatgggcg tagaagcttc cgcaactcac gtcaatgaaa
      841 tgagcaaccc cgggcgcgac tatccactgg ctatgttact gctgatggtg gcggcaatct
      901 gcttaagctc tgttggcggt ttgtctattg cgatggtcat tccgggtaat gaaatcaacc
      961 tctccgcagg ggtaatgcaa acctttaccg ttctgatgtc ccatgtggca ccggaaattg
     1021 agtggacggt tcgcgtgatc tccgcactgc tgttgctggg tgttctggcg gaaatcgcct
     1081 cctggattgt tggtccttct cgcgggatgt atgtcacagc gcagaaaaac ctgctgccag
     1141 cggcattcgc taaaatgaac aaaaatggcg taccggtaac gctggtcatt tcgcagctgg
     1201 tgattacttc tatcgcgttg atcatcctca ccaataccgg tggcggtaac aacatgtcct
     1261 tcctgatcgc actggcgctg acggtggtga tttatctgtg tgcttatttc atgctgttta
     1321 ttggctacat tgtgttggtt cttaaacatc ctgacttaaa acgcacattt aatatccctg
     1381 gtggtaaagg ggtgaaactg gtcgtggcaa ttgtcggtct gctgacttca attatggcgt
     1441 ttattgtttc cttcctgccg ccggataaca tccagggtga ttctaccgat atgtatgttg
     1501 aattactggt tgttagtttc ctggtggtac ttgccctgcc ctttattctc tatgctgttc
     1561 atgatcgtaa aggcaaagcg aataccggcg tcactctgga gccaatcaac agtcagaacg
     1621 caccaaaagg tcacttcttc ctgcacccgc gtgcacgttc accacactat attgtgatga
     1681 atgacaagaa acactaacca tgtctgaatg gttataagac aagggagcga taattcatcg
     1741 ctcccttttt cgtgcttgct gtcgtattga cttaaccggg ataagtacga gaatgagcgc
     1801 acatctgttt accggaaacc agcacatatg gatatctgct cccgaaacaa gaaattaacg
     1861 attagaagac cagcgatact ggttgcattg gcacttttac tgtgtagttg taaaagcacg
     1921 cctccagagt ccatggtgac accaccagca ggttcaaagc caccagccac gacgcaacaa
     1981 tcgtcacaac cgatgcgtgg catctggctg gccacggttt ctcgcctcga ctggccaccg
     2041 gtttcctcgg ttaacattag taatcccacc agccgagccc gtgtacaaca acaggcgatg
     2101 atcgacaaac tggatcatct ccaacgtctc ggcataaaca cggtcttttt ccaggtcaag
     2161 ccggacggta ccgccctgtg gccatcgaaa attttgccgt ggtccgatct tatgaccggt
     2221 aagattggtg aaaatccggg ttacgatccg ctgcaattca tgctcgacga agcccacaag
     2281 cgtgggatga aagtacacgc ctggtttaac ccctatcgcg tatcggttaa tacgaagccc
     2341 ggtactatca gggaactgaa tagcactctg tctcaacaac cggcgagcgt ctatgtgcaa
     2401 caccgtgact ggatcagaac gtctggcgat cgctttgtcc tcgacccggg catccctgag
     2461 gttcaggact ggatcacatc aatagtcgct gaagtggttt cccgctatcc ggtagatggc
     2521 gtgcagtttg acgactattt ctatactgaa tcaccgggtt cacggctaaa tgataacgaa
     2581 acgtaccgta aatacggagg cgcatttgcg tcaaaagcag actggcggcg caacaatact
     2641 cagcagttaa ttgcaaaggt atcacacacc attaaaagca ttaagccggg agtcgaattt
     2701 ggcgttagcc cggcaggcgt gtggcgtaac cgatcacacg atccgctcgg ttccgatacc
     2761 cgaggcgcgg cagcctatga cgaatcctac gcagacaccc gtcgatgggt ggaacaagga
     2821 ttgctggatt acattgctcc ccaaatttac tggccgttct cacggagtgc cgcgcgttat
     2881 gacgtgttgg caaaatggtg ggcggatgtc gttaaaccaa ccaggacccg cctgtatatc
     2941 ggtatcgcct tctataaagt gggtgaacct tcaaagatag agccagactg gatgattaac
     3001 ggcggcgtac cggaactgaa aaagcagctc gatcttaacg atgcggtacc agaaattagc
     3061 ggcaccatct tgttccgtga ggactatctg aataaaccgc agactcaaca agcggtcagc
     3121 tatctgcaaa gtcgttgggg cagttaaact accatgctta ctttgtaaca agccggtgct
     3181 ttacccaccg gcttgttaca ccttgtgaaa tatcccacta gtaatcatgc ttacataagt
     3241 caaattaacc acgcaattaa ccacacttag ttaaacagct ataaatccct aataaaaatg
     3301 tatgaatatg aaaaaccata cgatattcgc tgtcaccgaa aaataattac gatgggctat
     3361 actcttctga agaacagtag cgtagattga aactgtctta agtcgcttat ttcttttcag
     3421 tatatcttca tatttcagga gaatatggtg cagttagtcg atctcgcacg ttgcgtttcg
     3481 ttttgattcc gtctgatttc ccagccttat aagggtgtga attatggaga tgtattttaa
     3541 aagaatgaaa gatgagtgga ccggacttgt cgaacaggca gatcctctca ttcgtgctaa
     3601 agccgcagaa attgcccttg cgcatgctca ttatctgagt atcgagtttt atcgaattgt
     3661 ccgcatcgac ccgcatgccg aagaattctt gagtaatgaa caagttgagc ggcagttgaa
     3721 aagtgcgatg gaacgctgga ttattaacgt gctttctgcc caggttgacg atgtcgaaag
     3781 gctaatacaa atccagcata ccgtcgcgga agtgcatgcc cgcataggaa ttccggtaga
     3841 aattgtcgag atggggtttc gggtgctgaa aaaaatcctc tatccggtaa tcttctcttc
     3901 ggattattcc gccgcagaaa aacttcaggt ctaccatttc tcgattaaca gtattgatat
     3961 cgcgatggaa gtgatgaccc gcgcgtttac ctttagtgac agtagtgcct caaaggaaga
     4021 tgaaaactat cgtatcttct cgttactgga aaacgccgaa gaagaaaaag aacggcaaat
     4081 agcctcaata ctttcatggg aaatagatat tatctataaa gtcctgctgg attctgattt
     4141 aggcagcagt ttgcctttaa gccaggctga ttttggcctg tggtttaacc ataaaggtcg
     4201 acattatttt agcggcattg ccgaagtggg ccatatctcc cgtctgattc aggatttcga
     4261 cggtattttc aatcaaacca tgcgtaacac caggattttg aataacagaa gtttgcgggt
     4321 aaaatttcta ttacagataa gaaataccgt atcgcagatt attaccttgc tgcgtgaatt
     4381 gtttgaagaa gtatcgcgcc acgaagtcgg tatggatgta ctgacgaaat tacttaaccg
     4441 ccgtttcctg ccgactatct tcaaacgcga aattgcccat gccaaccgga ccggtacacc
     4501 gctgtcagtg ctgattattg acgttgataa attcaaagag atcaacgata cgtggggcca
     4561 taacactggc gatgaaattc tgcgtaaagt ctcattcctc tcgcagaaga gattggtgaa
     4621 atcgaaaata ttgggcgctg ggtcatcgcg gaagcttgcc gtcagttagc agaatggcgt
     4681 agccagaata ttcatatccc ggcgttatcc gtgaacttgt cggcgctgca ctttcgcagt
     4741 aatcagctgc ctaatcaggt gtctgatgca atgcaagcct ggggtattga cggccaccag
     4801 ctgacggtgg aaatcacgga aagcatgatg atggaacacg ataccgaaat ctttaagcgc
     4861 attcagatcc tgcgtgatat gggcgtgggt ttatcggtag atgattttgg cacgggcttt
     4921 tccggattat cccgcttagt cagtcttccg gtaacggaaa tcaaaattga caaaagcttt
     4981 gtcgatcgtt gtctgacgga aaaacgcatc ctggccttac ttgaagccat taccagcatt
     5041 gggcaaagcc tcaatttaac cgtcgtggcg gaaggggtcg aaaccaaaga gcaatttgag
     5101 atgctacgta agatccactg tcgcgttatt cagggatatt tcttttcccg cccccttccc
     5161 gccgaagaaa ttccaggctg gatgagcagc gtgttaccgc tgaaaatctg acaaattcct
     5221 ctcgcccgca ctcgcgggtt ttcctttaag gtgacactgt cacttaaata ctgtgatttc
     5281 agctacgatt ccgggagatt ccttccttaa ctttccctct tgcaccaaaa ctgcactacg
     5341 ctgcacaaat gttgatcaaa agttaaaaaa tataacttta agccagcatt ctgccgctta
     5401 tcccggcatg gcatgagatc tgcataagcg gaaagcgcag caatttttgt cttatacagg
     5461 aaccgtttat gtcggatacc accgaactgg ttgatttagc cgtgatcttc cctgatctgg
     5521 agatcgaatt gaaatatgcc tgcgttgata acatcacagg taaagctatt tatcagcaag
     5581 cgcgttgtct gctacataaa gatgcgatta ccgcgctggc gaaaagtatc agtatcgccc
     5641 agctgtcagg cttacaactg gtgatttacg atgcgtatcg cccacaacaa gcacaggcga
     5701 tgttgtggca agcctgccca gacccgcaat atgttgttga tgtgacggtc ggttctaatc
     5761 acagccgtgg cacggcgctc gacctgacgc ttcgtgatga cgtggaggtg gtgaatgatg
     5821 ctaagcgagg aaacgtccgc cgtacgccca caaaaacaaa cgcgatttaa cggtgcaaaa
     5881 ctggtatgga tgctgaaagg cagtccgctc accgtgaccg gcgcagtcat cattgtatta
     5941 atgcgactga tgatgatttt ttcaccgtgg ctggcgacgc atgatcccaa cgccattgat
     6001 ttaaccgccc gccttttgcc gccttctgcg gcgcactggt ttggcaccga tgaagtggga
     6061 cgcgatctgt ttagccgcgt actggtcggc agtcagcaat caattctcgc cggattagtg
     6121 gtggtcgcca ttgcgggtat gattggttcg ctactcggat gtctatccgg tgtgcttggc
     6181 ggacgcgcag acgccatcat catgcgcatc atggacatta tgctgtcgat tccttcgttg
     6241 gtactgacaa tggcactggc tgccgctctc gggccgagtt tatttaacgc catgctggcg
     6301 attgctattg tgcgaattcc cttttatgtg cgcttggcgc gggggcaagc attagttgta
     6361 cgccagtata cctatgttca ggcggcgaaa acctttggcg cgtctcgttg gcatctgatc
     6421 aactggcata ttttacgtaa ctccctaccg ccgctgatcg tgcaggcatc gctggatatc
     6481 ggtagcgcga ttttaatggc cgccacgttg ggatttattg gcctaggtgc tcaacaaccg
     6541 agtgctgaat ggggcgcgat ggtagcgaat ggtcgcaact atgtactcga tcagtggtgg
     6601 tattgcgcat ttccgggggc agcgattttg cttacctccg tcgggtttaa tctctttggt
     6661 gatggtattc gcgatctgct tgacccgaaa gcaggaggaa agcagtcatg acccaacccg
     6721 ttctggacat tcaacagctg catttgagtt tccccggttt taacggtgat gttcacgcgc
     6781 tcaacaatgt gtccttgcag attaaccgcg gcgaaattgt cggtctggtg ggagaatccg
     6841 gctcaggtaa atcagtcacc gcaatgctga ttatgcgtct gctaccgacg ggcagttatt
     6901 gcgtacatcg ggggcagatt tcactgctgg gagaagatgt tcttaacgct cgggaaaaac
     6961 agcttcgtca gtggcgcggc gcacgagtgg cgatgatctt tcaggaaccg atgaccgccc
     7021 ttaatccgac acgtcgaata ggtcttcaga tgatggacgt aatccgccat catcaaccaa
     7081 taagtcgtcg ggaagccaga gctaaagcga ttgccctgct ggaagagatg caaatcccgg
     7141 atgccgtgga agttatgtcg cgctatccgt ttgagctttc aggtggtatg cgccagcggg
     7201 taatgattgc gctggcattc tcctgcgagc cgcaattgat tattgccggc gaaccgacga
     7261 cggcgctgga cgtcacagta cagttgcagg tactgcgttt gcttaaacat aaagcacgcg
     7321 ccagtggaac tgcggtactg ttcatcagcc atgatatggc cgtggtgtcg caactgtgcg
     7381 atagcgttta cgtgatgtat gccggaagcg taattgaaag cggcgggacg gcagacgtta
     7441 tccatcatcc ccggcatccg tataccattg gtttgctgca atgcgcacca gaacatggag
     7501 taccacgcca gccattaccc gccattccag ggacggtacc aaacctcacc catttgcctg
     7561 acggctgcgc ttttcgcgat cgttgctatg cggcaggtgc acagtgtgaa aacgtcccgg
     7621 cgctgacagc gtgtggtgac aacaaccagc actgcgcctg ttggtatccg cagcaggagg
     7681 tcataagtgt ctgacacgtt attaacgtta cgcgacgtcc atatcaattt tccggcccgt
     7741 aaaaactggc ttggtaaatc tacggaacat gttcatgcca ttaatggtat tgatttacag
     7801 atccgccgtg gtgaaacctt agggatcgtc ggcgagtcag gctgcggcaa aagcaccctc
     7861 gcacagcttt taatgggcat gctgcaaccg agccacgggc agtacatccg ttcaggctca
     7921 caacgcatta tgcagatggt gtttcaggac ccgctctctt cgcttaatcc gcgcttaccg
     7981 gtgtggcgca tcatcacaga atcgctctgg atagctaagc gtagtagtga acaacagcgg
     8041 cgagcgttgg cagaggagtt ggctgtgcag gtgggtattc gtccggagta tctcgaccgc
     8101 ctgcctcatg cgttctccgg cgggcagcgg caacgcatcg ccattgccag agcactctct
     8161 tcgcagcctg acgtgattgt gcttgatgag ccaacctcgg cgctggatat ctccgtgcag
     8221 gcgcagatcc tcaatttgct ggtaacgcta caggaaaatc acgggctgac ttatgtgctg
     8281 atttcacaca atgtctcggt gatacgtcat atgagcgatc gggtggcggt gatgtatctc
     8341 gggcagattg tagaactggg cgacgcgcaa caggtgttga ctgcttcggc acatccatac
     8401 acccgattat tgctggattc cctccccgcc attgataaac cgctggagga agaatgagca
     8461 ttacgtaaaa cggatctgcc gggaaaccgc acgttgccgc aaggctgttt tttctacgaa
     8521 cgttgccctc tggcaaccca cggatgtgaa gtccggcaat cattagcaat aagggaggac
     8581 ggacgtgagc tccggtgctg gcgggcgctg tagagatgct aacaacgcat caggcattca
     8641 cgcgcctgac gcgtcatccg gcaatgcttt acgatttcaa ctggtaatcc agcgtaattt
     8701 ccgctttcag tacctgagag accgggcatc ctgcttttgc tttctggatg atgccgtcaa
     8761 aggtagaggc atcaataccc ggcaccgcaa cttcactctt cagtgcgatt ttcgtaatcg
     8821 caaaaccggc atccacttta tccagcgaca catcggcggt ggtatcaatt gatgttggcg
     8881 tgaatcccgc ttcccccagc attaatgaaa gcgccattga gaaacatgcg gcatgcgctg
     8941 cgccaatcag ttcttcaggg ttggttcctt tttcgccttc aaaacgcgtg ttaaatccat
     9001 acggctgttg gttcagcacg ccgctctcgg tggatactgt tcccttcccg cgtttgatat
     9061 cgccttccca gtgtgcctga cctttcttat ggattgtcat tgttgctctc ctgtgggctt
     9121 aaaaaggaaa ttatagacta cgtgagaaac agcacgagga taagcaggat attccgaata
     9181 aaatacggcg aaaatattac ttctgcactt aaattaaatt cccacaatag ataaccggga
     9241 atatatatct caagagaggt aatgactcca acttactgat agtgttttat gttcagataa
     9301 tgcccgatga ccttgtcatg cagctccacc gattttg
//
LOCUS       DAJTQY010000129         9153 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.129, whole genome shotgun sequence.
ACCESSION   DAJTQY010000129 DAJTQY010000000
VERSION     DAJTQY010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9153
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.129"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            <1..923
                     /locus_tag="OO349_003507"
     CDS             <1..923
                     /locus_tag="OO349_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5868446.1"
                     /translation="GIGEAPFMPAGVKSITDWYAQKERGTALGIFNSSTVIGQAIAPP
                     ALVLMQLAWGWRTMFVIIGVAGILVGICWYAWYRNRAQFVLTDEERTYLSASVKPRPQ
                     LQFSEWLALFKHRTTWGMILGFSGVNYTGWLYIAWLPGYLQAEQGFSLAKTGWVAAIP
                     FLAAAVGMWVNGIVVDRLAKKGYDLAKTRKTAIVCGLMMSALGTLLVVQSSSPAQAVA
                     FISMALFCVHFAGTSAWGLVQVMVSETKVASIAGIQNFGSFVFASFAPIVTGWVVDTT
                     HSFNLALVIAACVTFTGALCYFFIVKDRIE"
     gene            1175..2092
                     /gene="oxyR"
                     /locus_tag="OO349_003508"
     CDS             1175..2092
                     /gene="oxyR"
                     /locus_tag="OO349_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HCR5868447.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWENRECVLMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(2075..3475)
                     /gene="sthA"
                     /locus_tag="OO349_003509"
     CDS             complement(2075..3475)
                     /gene="sthA"
                     /locus_tag="OO349_003509"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HCR5868448.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIESCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            3796..4446
                     /gene="fabR"
                     /locus_tag="OO349_003510"
     CDS             3796..4446
                     /gene="fabR"
                     /locus_tag="OO349_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HCR5868449.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            4446..4805
                     /locus_tag="OO349_003511"
     CDS             4446..4805
                     /locus_tag="OO349_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HCR5868450.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMSVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4845..5933)
                     /gene="trmA"
                     /locus_tag="OO349_003512"
     CDS             complement(4845..5933)
                     /gene="trmA"
                     /locus_tag="OO349_003512"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HCR5868451.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRAQNLNVHLIGRATKTKIALD
                     QDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELYCGNG
                     NFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNGVREF
                     NRLQGIDLKSYQFETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLETLSQ
                     THKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      6047..6237
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            6302..8146
                     /gene="btuB"
                     /locus_tag="OO349_003513"
     CDS             6302..8146
                     /gene="btuB"
                     /locus_tag="OO349_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HCR5868452.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTAIRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRIEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            8091..8948
                     /gene="murI"
                     /locus_tag="OO349_003514"
     CDS             8091..8948
                     /gene="murI"
                     /locus_tag="OO349_003514"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCR5868453.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         2094 a         2217 c         2469 g         2373 t
ORIGIN      
        1 tgggtattgg cgaagcgcca tttatgcctg cgggagtaaa gtcgatcacc gactggtatg
       61 cacaaaaaga acgcggcacg gcgctgggga tttttaactc atctaccgtt atcggtcagg
      121 ctatcgcacc tcctgctctt gtattgatgc agctggcatg gggctggcgg acgatgttcg
      181 ttatcatcgg cgtggcaggg attctggttg ggatctgttg gtacgcgtgg tatcgcaacc
      241 gggcgcagtt tgtcctgact gacgaagaac ggacgtatct ctccgcgtcg gttaaaccgc
      301 gtccacagct gcaatttagc gagtggctgg cgctgtttaa gcatcggaca acctggggga
      361 tgattttagg tttctctggc gtcaattata ccggttggct ttacattgcg tggttacccg
      421 gttatttgca agccgaacaa ggtttcagtc tggcgaaaac cggttgggta gcggcgattc
      481 ctttcctcgc ggcggcggtc gggatgtggg ttaacggtat tgtggtcgat cggctggcga
      541 aaaaaggcta cgacctggcg aagacgcgta aaacggctat tgtctgcggt ttgatgatgt
      601 cggcattagg cacgttgctg gtcgtgcaat cttcctcgcc agctcaggcg gtagcgttta
      661 tctcaatggc gctgttctgc gtgcatttcg cgggaacgtc tgcatgggga ctggtgcagg
      721 tgatggtgtc agaaacaaaa gtggcttcca tcgccgggat tcaaaacttt ggcagttttg
      781 tttttgcttc ctttgctccg atcgtaaccg gttgggtagt ggataccaca cactcgttta
      841 atctggcgct ggttatcgcg gcctgtgtga cgttcaccgg ggcgctgtgt tacttcttta
      901 ttgtcaaaga tcgcattgag taatcgttac tatcaggctc tcttgctgtg ggcctgtaga
      961 ataaaaaaaa gcggatcctg gagatccgca aaagttcacg ttggctttag ttattcgagt
     1021 tgagaaactc tcgaaacggg cagtgacttc aagggttaaa agaggtgccg ctccgtttct
     1081 gtgagcaatt atcagtcaga atgcttgata gggataatcg ttcattgcta ttctacctat
     1141 cgccatgaac tatcgtggcg atggaggatg gataatgaat attcgtgatc ttgagtacct
     1201 ggtggcattg gctgaacacc gccattttcg gcgtgcggca gattcctgcc acgttagcca
     1261 gccgacgctt agcggccaaa ttcgtaagct ggaagatgag ctgggcgtga tgttgctgga
     1321 gcggaccagc cgtaaagtgt tgttcaccca ggcgggaatg ctgctggtgg atcaggcgcg
     1381 taccgtgctg cgtgaggtga aagtccttaa agagatggca agccagcagg gtgagacgat
     1441 gtccggaccg ctgcatattg gtttgattcc tacggttgga ccatacctgc taccgcatat
     1501 tatcccgatg ctgcaccaga catttccaaa gctggaaatg tatctgcatg aagcacagac
     1561 ccaccagtta ctggcgcaac tggacagcgg caaactcgat tgcgtgatcc tcgcgctggt
     1621 gaaagagagc gaagcattca ttgaagtgcc gttgtttgat gagccaatgt tgctggctat
     1681 ctatgaagat cacccgtggg agaaccgcga atgcgtactg atggccgatc tggcagggga
     1741 aaaactgctg atgctggaag atggtcactg tttgcgcgat caggcaatgg gtttctgctt
     1801 tgaagccggg gcggatgaag atacacactt ccgcgcgacc agcctggaaa ctctgcgcaa
     1861 catggtggcg gcaggtagcg ggatcacttt attgccagcg ctggctgtgc cgccggagcg
     1921 caaacgcgat ggggttgttt atctgccatg cattaagccg gaaccacgcc gcactattgg
     1981 cctggtttat cgtcctggct caccgctgcg cagccgctat gagcagctgg cagaggccat
     2041 ccgcgcaaga atggatggcc atttcgataa agtgttaaaa caggcggttt aaaccgttta
     2101 acgcagctac ccgataggct tccgccatcg tcgggtagtt aaaggtggtg ttgacgaagt
     2161 actcaatagt gttgccgcca cctttctgtt ccataatcgc ctgaccgata tgaataattt
     2221 cggcagcgcg ctcgccaaag cagtgaatac ccagaatctc ttttgtttcc cgatggaaca
     2281 aaattttcag cgtgcccacg ttcatgccga cgatttgtgc acgtgccaga tgtttgaact
     2341 gggcgcggcc cacttcatac ggcactttca tcgcggtcag ctgctgttcg gttttgccca
     2401 cagagctgat ttccgggatg gtgtaaatgc cggtagggat atcttcaatc agatgtgcgg
     2461 tggcttcgcc ttttaccagc gcctgcgcgg caatgcgccc ctggtcatag gccgctgatg
     2521 ccaggctcgg ataaccaatc acgtcgccca ccgcgtaaac gtgcggctgt gcggtctgat
     2581 acatgctgtt gaccttcagc tgtccgcgac tgtcagtttc cagcccaatg ttctgtaacg
     2641 ccagcgaatc ggtattaccg gtacgaccgt tggcatagag caggcagtca gctttcagtt
     2701 ttttacccga cttcagatgc atgatcacac cgtcgtcaca gctttcgatc ttctcgtact
     2761 cttcgttgtg acgaatcact acgccactgt tccagaagtg gtaggagaga gaatctgaca
     2821 tctcttgatc gagaaatgcc agcaggcgat cgcgggtgtt gatcagatcc acttttacat
     2881 ccataccgcg gaagatcgac gcatattcac agccgatcac tccagcacca tagataagta
     2941 catggcgcgg ttcgtggtgc atgctaagaa ttgagtcgct gtcgtaaatg cgtggatggg
     3001 tgaaatcaac atctgttgga tgatatggac gagagccgca ggcaataaca aatttttcag
     3061 cggttagtgt ttcaacgctg ccgtccgggc aatccagcgc caacgtatgc tcgtcaacaa
     3121 agcgagcgtt cccctgcaat atttcacagt gattacgttc gtaaaatccc tgacgcatgc
     3181 gcgtttgttg attaatcacg ttatcggcat ggttaaggat atcggcaaaa gaagagcgga
     3241 gcagtcggga atggtcgctg tatagtgggt tttgattgaa ttctataatg cggctgacgg
     3301 cgtgacggag agctttcgac gggatggtgc cccagtgggt gcaaccgccg ccaacatttt
     3361 gataacgctc gataactgcg acgcgcgcac cttgcttaac caggcccatt gcagcgcctt
     3421 cgccgccggg gccggaacct attactatgg catcgtaatc gtaggaatgt ggcatggtag
     3481 ggcttacctg ttcttataca taaaagcaac agaatggtaa cattctatcg cgggtaagcc
     3541 aattgatccc cgtcatttat ctggctatat cctgagcggc ctttgctttg tctgtttctt
     3601 acttttgccc tgacgtttta ttggattttt atcgacgata ctctccgttt aagcggcagg
     3661 tttccgctgt acgtaaaaga accggccagt aaatatgttt tattgcgtta ccgttcattc
     3721 acaatactgg agcaatccag tatgttcatt ctctggtata gtgccagcag tacttttggc
     3781 aaggattcag acatcgtgat gggcgtaaga gcgcaacaaa aagaaaaaac ccgccgttcg
     3841 ctggtggaag ccgcatttag ccaattaagt gctgaacgca gcttcgccag cctgagtttg
     3901 cgtgaagtgg cgcgtgaagc gggcattgct cccacctctt tttatcggca tttccgcgac
     3961 gtagacgaac tgggtctgac catggttgat gagagcggtt taatgctacg ccaactcatg
     4021 cgccaggcgc gtcagcgtat cgccaaaggc gggagtgtga tccgtacctc ggtctccaca
     4081 tttatggagt tcatcggtaa taatcctaac gccttccggt tattattgcg ggaacgctcc
     4141 ggcacctccg ctgcgtttcg tgccgccgtt gcgcgtgaaa ttcagcactt cattgcggaa
     4201 cttgcggact atctggaact cgaaaaccat atgccgcgtg cgtttactga agcgcaagcc
     4261 gaagcaatgg tgacaattgt cttcagtgcg ggtgccgagg cgttggacgt cggcgtcgaa
     4321 caacgtcggc aattagaaga gcgactggta ctgcaactgc gaatgatttc gaaaggggct
     4381 tattactggt atcgccgtga acaagagaaa accgcaatta ttccgggaaa tgtgaaggac
     4441 gagtaatgaa acaagcaaat caagatagag gtacgctgct gctggcgttg gttgctggtt
     4501 tatcgattaa tggtactttc gcagcgctgt ttagctccat tgtgccattt tctgtattcc
     4561 cgattatttc cctggtgctg acggtttact gcctgcatca acgttatctt aatcgcacca
     4621 tgtcggtagg cttgccgggt ctggcagctg cctgttttat tctcggcgta ctgctgtaca
     4681 gcacggtagt tcgtgcggaa tatccggata tcggctctaa cttcttcccg gcagtactct
     4741 ccgtcattat ggtgttctgg attggcgcga agatgcgtaa ccgtaagcag gaagttgctg
     4801 agtaatcggt atcatgccgg atgctggcgc atccggcatg ggttttactt cgcggtcagt
     4861 aatacgccgc actccatatg gtgcgtgtag gggaactgat caaacagagc cagacgttcg
     4921 accttgtgcg tctggcttaa tgtttccaga tttttgcata acgtttccgg attgcaggag
     4981 atatacagaa tacgtggata cgcctgcacc attttctcgg tttcactgtc cagaccgctg
     5041 cgcggtgggt cgacaaaaat ggtttcgaac tgatagctct ttaagtcgat cccttgcagg
     5101 cggttaaact cgcgcacacc attcatcgcc tgagtaaatt cttctgccgc catacgaata
     5161 atttgtacgt tatcaatatg gttagcggca atgttgtatt gcgcggcagc gactgacggt
     5221 ttggcgattt cggtggctaa tacccgatca aaattgcgcg ccagcgctaa tgaaaagtta
     5281 ccgttgccgc agtacagctc cagtaaatcg ccttttgagc ctttggttac gtccagcgcc
     5341 cattccagca tctgaatatt catcgccgcg ttcggctggg taaagctgtt ttctacctga
     5401 cgatagatca tctctttccc tgcgaccggc agacgctcgt cgatgtaatc ttgatccagc
     5461 gcgattttgg ttttcgttgc ccgaccaatc agatgcacat tcagattctg cgcgcgcagt
     5521 gcttccgctt cctgacgcca ctcatcatcc agctttttat ggtacagcag ggaaaccacc
     5581 gcctgattac tcagcgtggt gaggtaatca atctggaaca acttgtggcg cagaacggga
     5641 ttattacgca cacccgcaat catcgccgtc atcaactggt tgatgagttc actggcggcg
     5701 gggaagctat ccacgcggat gcggcttttg gtttgttgat cgaaaatgat gtgatacagg
     5761 tcatcgccat cgtgccagat gcggaactcc gcgcgcatcc ggtaatgact gaccggcgag
     5821 cgaaacactt ccggaaccag gtcagaaaac ggtgccatca tactttgcaa acgtaccact
     5881 ttttcggcta actgcgcttc atactgttct gttggaaggt gttcgggggt catgatgtat
     5941 cctgaaaaat taaagtacgc ggcgattgta gggattgctc atcagatgtc cagatcttga
     6001 tgaattccta tttgtgagct acgtctggac agtaacttgt tacaacctgt agcatccact
     6061 tgccggtcct gtgagttaat agggaatcca gtgcgaatct agagctgacg cgcagcggta
     6121 aggaaaggtg cgatgattgc gttatgcgga cactgccatc cggtgggaag tcatcatctc
     6181 ttagtatctt agatacccct ccaagcccga agacctgccg gccaacgtcg catctggttc
     6241 tcatcatcgc gtaatattga tgaaacctgc ggcatccttc ttctattgtg gatgctttac
     6301 aatgattaaa aaagcttcgc tgctgacggc gtgttccgtc acggcatttt ccgcttgggc
     6361 acaggatacc agcccggata ctctcgtcgt tactgctatc cgttttgaac agccgcgcag
     6421 cactgtgctt gcaccaacca ccgttgtgac ccgtcaggat atcgaccgct ggcagtcgac
     6481 ctcggtcaat gatgtgctgc gccgtcttcc gggcgtcgat atcacccaaa acggcggttc
     6541 aggtcagctc tcatctattt ttattcgcgg tacaaatgcc agtcatgtgc tggtgttaat
     6601 tgatggcgtc cgcctgaatc tggcgggggt gagtggttct gccgacctta gccagttccc
     6661 tattgcgctt gtccagcgta ttgaatatat ccgtggaccg cgctccgctg tttatggttc
     6721 cgatgcaata ggcggggtgg tgaatatcat cacgacgcgc gatgaacccg gaacggaaat
     6781 ttcagcaggg tggggaagca atagttatca gaactatgat gtctctacgc agcaacaact
     6841 gggggataaa acacgggtaa cgctgttggg cgattatgcc catactcatg gttatgatgt
     6901 tgttgcctat ggtaataccg gaacgcaagc gcagacagat aacgatggtt ttttaagtaa
     6961 aacgctttat ggggcgctgg agcataactt tactgatgcc tggagcggct ttgttcgcgg
     7021 ctatggctat gataaccgta ccaattatga cgcgtattat tctcccggtt caccgttgct
     7081 cgatacccgt aaactctata gccaaagttg ggacgccggg ctgcgctata acggcgaact
     7141 gattaaatca caactcatta ccagctatag ccatagcaaa gattacaact acgatcccca
     7201 ttatggtcgt tatgattcgt cggcgacgct cgatgagatg aagcaataca ccgtccagtg
     7261 ggcaaacaat gtcatcgttg gtcacggtag tattggtgcg ggtgtcgact ggcagaaaca
     7321 gactacgacg ccgggtacag gttatgttga ggatggatat gatcaacgta ataccggcat
     7381 ctatctgacc gggctgcaac aagtcggcga ttttaccttt gaaggcgcag cacgcagcga
     7441 cgataactca cagtttggtc gtcatggaac ctggcaaacc agcgccggtt gggaattcat
     7501 cgaaggttat cgcttcattg cttcctacgg gacatcttat aaggcaccaa atctggggca
     7561 actgtatggc ttctacggaa atccgaatct ggacccggag aaaagcaaac agtgggaagg
     7621 cgcgtttgaa ggcttaaccg ctggggtgaa ctggcgtatt tccggatatc gtaacgatgt
     7681 cagtgacttg atcgattatg atgatcacac cctgaaatat tacaacgaag ggaaagcgcg
     7741 gattaagggc gtcgaggcga cagccaattt tgataccgga ccactgacgc atactgtgag
     7801 ttatgattat gtcgatgcgc gcaatgcgat taccgacacg ccgttgttac gccgtgctaa
     7861 acagcaggtg aaataccagc tcgactggca gttgtatgac ttcgactggg gtattactta
     7921 tcagtattta ggcactcgct atgataagga ttactcatct tatccttatc aaaccgttaa
     7981 aatgggcggt gtgagcttgt gggatcttgc ggttgcgtat ccggtcacct ctcacctgac
     8041 agttcgtggt aaaatagcca acctgttcga caaagattat gagacagtct atggctacca
     8101 aactgcagga cgggaataca ccttgtctgg cagctacacc ttctgaacca cgtcccaccg
     8161 tgctggtgtt tgactccggc gtcggtgggt tgtcggtcta tgacgagatc cggcatctct
     8221 taccggatct ccattacatt tatgctttcg ataacgtcgc tttcccgtat ggcgaaaaaa
     8281 gcgaagcgtt tattgttgag cgagtggtgg caattgtcac cgcggtgcaa gaacgttatc
     8341 cccttgcgct ggctgtggtc gcttgcaaca ccgccagtac cgtttcactt cctgcattac
     8401 gcgaaaagtt cgacttcccg gttgttggtg tcgtgccggc gattaaacct gctgcacgtc
     8461 tgacggcaaa tggcattgtc ggattactgg caacccgcgg aacagttaaa cgttcttata
     8521 ctcatgagct gatcgcgcgt ttcgctaatg aatgccagat agaaatgctg ggctcggcag
     8581 agatggttga gttggctgaa gcgaagctgc atggcgaaga tgtttctctg gatgcactaa
     8641 aacgtatcct acgcccgtgg ttaagaatga aagagccgcc agataccgtt gtattgggtt
     8701 gcacccattt ccctctacta caagaagaac tgttacaagt gctgccagag ggaacccggc
     8761 tggtggattc tggcgcagcg attgctcgcc gaacggcctg gttgttagaa catgaagccc
     8821 cggatgcaaa atctgccgat gcgaatattg ccttttgtat ggcaatgacg ccagaagctg
     8881 aacaattatt gcccgtttta cagcgttacg gcttcgaaac gctcgaaaaa ctggcagttt
     8941 taggctgatt tggttgaatg ttgcgcggtc ggaaaattat tttaaatttc ctcttgtcag
     9001 gccggaataa ctccctataa tgcgccacca ctgacacgga acaacggcaa acacgccgac
     9061 gggtcagcgg ggttctcctg agaattccga cagagaaagc aaaaataaat gcttgactct
     9121 gtagcgggaa agcgtattat gcacaccccg cgc
//
LOCUS       DAJTQY010000130         9061 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.130, whole genome shotgun sequence.
ACCESSION   DAJTQY010000130 DAJTQY010000000
VERSION     DAJTQY010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9061
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.130"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            <1..1292
                     /locus_tag="OO349_003515"
     CDS             <1..1292
                     /locus_tag="OO349_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TniQ family protein"
                     /protein_id="HCR5868454.1"
                     /translation="YAPFVGKERRDEAIRLMEYQAQGAVHLMLGVAASRVKSDNRFRY
                     CPDCVALQLNRYGEAFWQRDWYLPALPYCPKHGALVFFDRAVDDHRHQFWALGHTELL
                     SDYPKDSLSQLTALAAYIAPLLDAPRAQELSPSLEQWTLFYQRLAQDLGLTKSKHIRH
                     DLVAERVRQTFSDEALEKLDLKLAENKDTCWLKSIFRKHRKAFSYLQHSIVWQALLPK
                     LTVIEALQQASALTEHSITTRPVSQSVQPNSEDLSVKHKDWQQLVHKYQGIKAARQSL
                     EGGVLYAWLYRHDRDWLVHWNQQHQQERLAPAPRVDWNQRDRIAVRQLLRIIKRLDSS
                     LDHPRATSSWLLKQTPNGTSLAKNLQKLPLVALCLKRYSESVEDYQIRRISQAFIKLK
                     QEDVELRRWRLLRSATLSKERITEEAQRFLEMVYGEE"
     gene            1293..2909
                     /locus_tag="OO349_003516"
     CDS             1293..2909
                     /locus_tag="OO349_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator"
                     /protein_id="HCR5868455.1"
                     /translation="MVRLATFNDNVQVVHIGHLFRNSGHKEWRIFVWFNPMQERKWTR
                     FTHLPLLSRAKVVNSTTKQINKADRVIEFEASDLQRAKIIDFPNLSSFASVRNKDGAQ
                     SSFIYEAETPYSKTRYHIPQLELARSLFLINSYFCRSCLSSTALQQEFDVQYEVERDH
                     LEIRILPSSSFPKGALEQSAVVQLLVWLFSDQDVMDSYESIFRHYQQNREIKNGVESW
                     CFSFDPPPMQGWKLHVKGRSSNEDKDYLVEEIVGLEINAMLPSTTAISHASFQEKEAG
                     DGSTQHIAVSTESVVDDEHLQLDDEETANIDTDTRVIEAEPTWISFSRPSRIEKSRRA
                     RKSSQTILEKEEATTSENSNLVSTDEPHLGGVLAAADVGGKQDATNYNSIFANRFAAF
                     DELLSILKTKFACRVLFEETLVLPKVGRSRLHLCKDGSPRVIKAVGVQRNGSEFVLLE
                     VDASDGVKMLSTKVLSGVDSETWRNDFEKIRRGVVKSSLNWPNSLFDQLYGQDGHRGV
                     NHPKGLGELQVSREDMEGWAERVVREQFTH"
     gene            complement(2965..3117)
                     /locus_tag="OO349_003517"
                     /pseudo
     CDS             complement(2965..3117)
                     /locus_tag="OO349_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069623.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(3140..3517)
                     /locus_tag="OO349_003518"
     CDS             complement(3140..3517)
                     /locus_tag="OO349_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5868456.1"
                     /translation="MSAAKALAYALCDKLESKKVDEVVRNLVSSGWNIREVAWEASSF
                     DHEMPYFDTGRDFDIGFPDTLSSKIEFLTANGSIVQARARLHFKQALIFAKASKHVAA
                     LKNSLDYYYGSSVIPPQNHGGYL"
     gene            complement(3927..4298)
                     /locus_tag="OO349_003519"
     CDS             complement(3927..4298)
                     /locus_tag="OO349_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5868457.1"
                     /translation="MSYEPPRDGYTNGYIVDVMYDNRPSKYPAVFRTYVRIPDYVELD
                     NAEELADAIIREVELKEAWLTRVLGNSADPDASVGKWDRRLAQCGGIDEMSSGVQATY
                     FSPNGQLVGLVAYIDAEWMDE"
     gene            complement(4359..4793)
                     /locus_tag="OO349_003520"
     CDS             complement(4359..4793)
                     /locus_tag="OO349_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5868458.1"
                     /translation="MRDLLKVVLSLVLAMASGQALADLPIVLVDEARLPYDYSPSNYD
                     ISPSNYDNSSSNYDNSRNGNRRLIYSANGSRTFAGYYVIANNGTTNFFSTSGKRMFYT
                     PKGGRGVYGGKDGSFCGALVVINGQFSLALTDNGLKIMYLSN"
     gene            complement(4869..5657)
                     /gene="aadA1"
                     /locus_tag="OO349_003521"
     CDS             complement(4869..5657)
                     /gene="aadA1"
                     /locus_tag="OO349_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR5868459.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     gene            complement(5715..6239)
                     /gene="sat2"
                     /locus_tag="OO349_003522"
     CDS             complement(5715..6239)
                     /gene="sat2"
                     /locus_tag="OO349_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HCR5868460.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            complement(6334..6807)
                     /locus_tag="OO349_003523"
     CDS             complement(6334..6807)
                     /locus_tag="OO349_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HCR5868461.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            7139..7675
                     /locus_tag="OO349_003524"
     CDS             7139..7675
                     /locus_tag="OO349_003524"
                     /inference="COORDINATES: protein motif:HMM:NF024886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
                     /protein_id="HCR5868462.1"
                     /translation="MSNSPFLNSIRTDMRQKGYALKTEKTYLHWIKRFILFHKKRHPQ
                     TMGSEEVRLFLSSLANSRHVAINTQKIALNALAFLYNRFLQQPLGDIDYIPASKPRRL
                     PSVISANEVQRILQVMDTRNQVIFTLLYGAGLRINECLRLRVKDFDFDNGCITVHDGK
                     GGKSRNSLLPTRLIPAIK"
     gene            7790..8116
                     /locus_tag="OO349_003525"
     CDS             7790..8116
                     /locus_tag="OO349_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5868463.1"
                     /translation="MFVFPSSTLCNHPYNGKLCRHHLHDSVARKALKAAVQKAGIVSK
                     RVTCHTFRHSFATHLLQAGRDIRTVQELLGHNDVKTTQIYTHVLGQHFAGTTSPADGL
                     MLLINQ"
     gene            complement(8304..8543)
                     /locus_tag="OO349_003526"
     CDS             complement(8304..8543)
                     /locus_tag="OO349_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HCR5868464.1"
                     /translation="MTPFRYNSDLTSGSLQTRECRIITGLLLQELDEAAWDKAMYKEN
                     VLQKRTQSTVRRISSALRKRLEHLSSDFWAFAFLC"
BASE COUNT         2465 a         2001 c         2119 g         2476 t
ORIGIN      
        1 tatatgctcc gtttgtaggc aaggagcgcc gagacgaagc tattcggtta atggagtacc
       61 aagcgcaagg tgcggtgcat ttaatgctag gagtcgctgc ttctagagtt aagagcgata
      121 accgctttag atactgccct gattgcgttg ctcttcagct aaataggtat ggggaagcct
      181 tttggcaacg agattggtat ttgcccgctt tgccatattg tccaaaacac ggtgctttag
      241 tcttctttga tagagctgta gatgatcacc gacatcaatt ttgggctttg ggtcatactg
      301 agctgctttc agactacccc aaagactccc tatctcaatt aacagcacta gctgcttata
      361 tagcccctct gttagatgct ccacgagcgc aagagctttc cccaagcctt gagcagtgga
      421 cgctgtttta tcagcgctta gcgcaggatc tagggctaac caaaagcaag cacattcgtc
      481 atgacttggt ggcggagaga gtgaggcaga cttttagtga tgaggcacta gagaaactgg
      541 atttaaagtt ggcagagaac aaggacacgt gttggctgaa aagtatattc cgtaagcata
      601 gaaaagcctt tagttattta cagcatagta ttgtgtggca agccttattg ccaaaactaa
      661 cggttataga agcgctacag caggcaagtg ctcttactga gcactctata acgacaagac
      721 ctgttagcca gtctgtgcaa cctaactctg aagatttatc tgttaagcat aaagactggc
      781 agcaactagt gcataaatac caaggaatta aggcggcaag acagtcttta gagggtgggg
      841 tgctatacgc ttggctttac cgacatgaca gggattggct agttcactgg aatcaacagc
      901 atcaacaaga gcgtctggca cccgccccta gagttgattg gaaccaaaga gatcgaattg
      961 ctgtacgaca actattaaga atcataaagc gtctagatag tagccttgat cacccaagag
     1021 cgacatcgag ctggctgtta aagcaaactc ctaacggaac ctctcttgca aaaaatctac
     1081 agaaactgcc tttggtagcg ctttgcttaa agcgttactc agagagtgtg gaagattatc
     1141 aaattagacg gattagccaa gcttttatta agcttaaaca ggaagatgtt gagcttaggc
     1201 gctggcgatt attaagaagt gcaacgttat ctaaagagcg gataactgag gaagcacaaa
     1261 gattcttgga aatggtttat ggggaagagt gagtggttag gctagctaca tttaatgaca
     1321 atgtgcaggt tgtacatatt ggtcatttat tccgtaactc gggtcataag gagtggcgta
     1381 tttttgtttg gtttaatcca atgcaagaac ggaaatggac tcgatttact catttgcctt
     1441 tattaagtcg agctaaggtg gttaacagta caacaaagca aataaataag gcggatcgtg
     1501 tgattgagtt tgaagcatcg gatcttcaac gagccaaaat aatcgatttt cctaatctct
     1561 cgtcctttgc ttccgtacgc aacaaggatg gagcgcagag ttcatttatt tacgaagctg
     1621 aaacaccata tagcaagact cgttatcaca tcccacagtt agagctagct cggtcattat
     1681 ttttaattaa ctcctatttc tgtcgaagct gtttgagcag taccgcttta cagcaagagt
     1741 tcgacgttca gtatgaggtt gagcgagatc atttagagat aaggatctta cccagttcat
     1801 cgtttcctaa aggggcgtta gagcagtcgg ccgtagtgca gcttttggtt tggttgtttt
     1861 cggatcaaga tgttatggat tcgtatgaaa gtatttttag gcactatcaa caaaatagag
     1921 aaattaagaa cggcgttgaa agctggtgct ttagctttga ccctccgccc atgcagggtt
     1981 ggaaattaca tgtaaaagga cgttcttcta acgaggataa ggattattta gttgaggaaa
     2041 tagtaggttt agaaatcaac gctatgcttc ctagcacaac agctattagc catgcctctt
     2101 ttcaggaaaa ggaggcaggt gatggtagta cgcagcacat agcggtttca acagagtcag
     2161 ttgttgatga tgagcatcta cagttggacg atgaggaaac agccaatata gacacagaca
     2221 cacgagtcat agaggctgag ccgacatgga taagttttag tagacctagt cgaattgaaa
     2281 aatctcgcag ggcaagaaaa agtagccaaa ctattttaga aaaagaagaa gcaacaacaa
     2341 gtgaaaatag taatttggtt agtactgatg agccacactt aggtggtgtc ctagcagcgg
     2401 cagatgtggg tgggaagcag gatgcaacca attacaactc tatttttgct aatcgatttg
     2461 ctgcttttga tgagctactt tcaattctaa aaactaaatt tgcatgtcgg gtgctttttg
     2521 aagaaacctt ggttttgcca aaagttgggc gtagccgatt acatctgtgt aaagatggct
     2581 caccaagagt gattaaagcc gttggggtgc aacgtaatgg cagtgaattt gtattgctag
     2641 aggtggatgc atcggatggg gtgaaaatgc tttctaccaa agtgttgagt ggcgttgata
     2701 gcgaaacatg gcggaatgat tttgaaaaga tacggcgtgg agtggtgaag agctcattga
     2761 attggccaaa tagtttgttt gatcaattat atggacaaga cgggcataga ggggtgaatc
     2821 atccaaaggg gttgggggag ctgcaagtat cgagagagga tatggaaggg tgggctgaga
     2881 gagtggttag agagcaattt acgcattaaa ggaatgactg aaagagcctg taaacccttt
     2941 tgtgtaagtg cttttgccgg tcagttaaag gtggccattt aaacggtcac caaattcgat
     3001 cataaaacgg ttcatggccg gcttccagtt gcggatcggc atcgtccatt tcttggtcgc
     3061 cgcctggata gccaggtaca ccaccttcat cgctgattcg tccgtaggga acaccttgag
     3121 ttcatattaa tggaattttc tacaaatagc ctccgtggtt ttgagggggg attacagacg
     3181 atccatagta gtaatccaat gagttcttga gcgcggcgac atgtttggac gccttggcaa
     3241 aaattagagc ctgcttgaag tgcaggcgag cacgtgcttg gacgattgat ccattcgcgg
     3301 tcaaaaactc aatcttggat gacagcgtgt caggaaatcc aatatcgaag tcccgccccg
     3361 tgtcaaagta gggcatttca tgatcaaagg acgaagcttc ccaggccact tccctaatat
     3421 tccagccgct agaaacaaga tttcgtacga cttcatcaac ttttttgctt tccaacttgt
     3481 cacaaagtgc ataggcaaga gctttggcgg cagacacgtg cgtttcctct taaacatagg
     3541 tcgaacggat acccgaggca aagcggagca ttagtcccaa agcatctccg gtagatccgg
     3601 aggctccata ctgaaggcat ggcatcttgg gcattgatat ttgaaacttc tctctagata
     3661 gcagggattt cttggccttg cctcatgctg atagcttgta aatatcacac gatccttagc
     3721 cccacaaagt gagcatactc cgtagctttc atttatagaa atcgtcgtta gattgtcgca
     3781 gcatgtgcac gttccgacta tttgctcagg aagcatcgcc ataccttggc ctaggtaaag
     3841 aagatcagac tcgtagccac atcggtggca actgagcttg tacgcaatgc ccatctggtt
     3901 gtctcacctg aatcgtaacg tcagagctat tcatccatcc attcggcatc aatgtatgcc
     3961 actaagccga ccagctgacc gtttggactg aagtatgttg cctgcacccc acttgacatc
     4021 tcatcaatac caccgcactg cgctaggcgc cggtcccact tgcccacaga agcgtcagga
     4081 tcggctgagt tccccaagac ccttgtgagc catgcctcct ttaactcaac ctctcgaatg
     4141 attgcgtcgg ccagctcctc cgcgttatct agttcgacat agtctggaat tcggacgtac
     4201 gttctgaaca cagctggata cttacttggg cgattgtcat acatgacatc aacaatgtac
     4261 ccgtttgtgt aaccgtctct tggaggttcg tatgacacta gtggttcccc tcagcttgcg
     4321 actagatgtt gaggcctaac attttattag agagcaggct agttgcttag atacatgatc
     4381 ttcaggccgt tatctgtcag ggcaagcgaa aattggccat ttatgacgac caatgccccg
     4441 cagaagctcc catctttgcc gccatagacg ccgcgccccc cttttggggt gtagaacatc
     4501 cttttgccag atgtggaaaa gaagttcgtt gtcccattgt tggcaatgac gtagtagccg
     4561 gcgaaagtgc gagacccatt tgcgctatat ataagcctac gatttccgtt gcgactattg
     4621 tcgtaattgg atgaactatt atcgtagttg cttggagaaa tgtcgtagtt ggatggggag
     4681 tagtcatagg gaagacgagc ttcatccact aaaacaattg gcaggtcagc aagtgcctgc
     4741 cccgatgcca tcgcaagtac gaggcttaga accaccttca acagatcgcg catagtcttc
     4801 cccagctctc taacgcttga gttaagccgc gccgcgaagc ggcgtcggct tgaacgaatt
     4861 gttagacatt atttgccgac taccttggtg atctcgcctt tcacgtagtg aacaaattct
     4921 tccaactgat ctgcgcgcga ggccaagcga tcttcttgtc caagataagc ctgcctagct
     4981 tcaagtatga cgggctgata ctgggccggc aggcgctcca ttgcccagtc ggcagcgaca
     5041 tccttcggcg cgattttgcc ggttactgcg ctgtaccaaa tgcgggacaa cgtaagcact
     5101 acatttcgct catcgccagc ccagtcgggc ggcgagttcc atagcgttaa ggtttcattt
     5161 agcgcctcaa atagatcctg ttcaggaacc ggatcaaaga gttcctccgc cgctggacct
     5221 accaaggcaa cgctatgttc tcttgctttt gtcagcaaga tagccagatc aatgtcgatc
     5281 gtggctggct cgaagatacc tgcaagaatg tcattgcgct gccattctcc aaattgcagt
     5341 tcgcgcttag ctggataacg ccacggaatg atgtcgtcgt gcacaacaat ggtgacttct
     5401 acagcgcgga gaatctcgct ctctccaggg gaagccgaag tttccaaaag gtcgttgatc
     5461 aaagctcgcc gcgttgtttc atcaagcctt acggtcaccg taaccagcaa atcaatatca
     5521 ctgtgtggct tcaggccgcc atccactgcg gagccgtaca aatgtacggc cagcaacgtc
     5581 ggttcgagat ggcgctcgat gacgccaact acctctgata gttgagtcga tacttcggcg
     5641 atcaccgctt ccctcatgat gtttaactcc tgaattaagc cgcgccgcga agcggtgtcg
     5701 gcttgaatga attgttaggc gtcatcctgt gctcccgaga accagtacca gtacatcgct
     5761 gtttcgttcg agacttgagg tctagtttta tacgtgaaca ggtcaatgcc gccgagagta
     5821 aagccacatt ttgcgtacaa attgcaggca ggtacattgt tcgtttgtgt ctctaatcgt
     5881 atgccaagga gctgtctgct tagtgcccac tttttcgcaa attcgatgag actgtgcgcg
     5941 actcctttgc ctcggtgcgt gtgcgacaca acaatgtgtt cgatagaggc tagatcgttc
     6001 catgttgagt tgagttcaat cttcccgaca agctcttggt cgatgaatgc gccatagcaa
     6061 gcagagtctt catcagagtc atcatccgag atgtaatcct tccggtaggg gctcacactt
     6121 ctggtagata gttcaaagcc ttggtcggat aggtgcacat cgaacacttc acgaacaatg
     6181 aaatggttct cagcatccaa tgtttccgcc acctgctcag ggatcaccga aatcttcata
     6241 tgacgcctaa cgcctggcac agcggatcgc aaacctggcg cggcttttgg tacaaaaggc
     6301 gtgacaggtt tgcgaatccg ttgctgccac ttgttaaccc ttttgccaga tttggtaact
     6361 ataatttatg ttagaggcga agtcttgggt aaaaactggc ctaaaattgc tggggatttc
     6421 aggaaagtaa acatcacctt ccggctcgat gtctattgta gatatatgta gtgtatctac
     6481 ttgatcgatc aggcttttgt atatctcccc accacctgaa acaatgacat gatccgttat
     6541 tttctttagg ttggttaaag catctttaat tgatggaaag atcaatacgt tctcattgtc
     6601 agatgtaaaa cttgaacgtg ttacgaccgc atactttcgg ttgggtaatg ctcccattga
     6661 ttcaaaagtc ttgcgtccaa ccaacagcca ttggttatag gtaatagctt taaacaggag
     6721 ctgttcacct ttggcactcc atggaatatc agggccattc ccgataactc cattcttcga
     6781 tatagctacc attagtgata gtttcacaat tcttcctcag aggttaacat tttattagta
     6841 agcatgctcg ttttgtcacc cgctgatgct taccgttaat taatataatc acttgtatta
     6901 aatagacttt ctttaaaaat acaagacact ctgttattac aaatcgtgca tgccgtctat
     6961 cctgcttgca cgatgcacat tatctcaccc aaagaactca taacatttta atttatttag
     7021 cattaaatta aaactctacc ggtaaacaag catctctagg cgtaaaaaac aggcactttt
     7081 ttaaatccca gctagaatag gctgtatagg cagacagttg caagacaagg agcgttttat
     7141 gtctaacagt ccatttttaa attctatacg cacggatatg cgacaaaaag gttatgcgct
     7201 gaaaactgaa aaaacttacc tgcactggat taagcgtttt attctgtttc acaaaaaacg
     7261 tcatcctcag accatgggca gtgaagaggt caggctgttt ttatccagct tagcaaacag
     7321 cagacatgta gccataaaca cgcagaaaat cgctttaaat gccctagctt ttttgtacaa
     7381 caggttttta caacagccgt tgggcgatat tgattatatc cctgcaagca agcctagacg
     7441 gctaccctct gttatctctg caaatgaagt gcaacgcatt ttgcaggtta tggatactcg
     7501 caaccaagtt atttttacgc tgctgtatgg tgcaggtttg cgcattaatg aatgcttgcg
     7561 tttgcgggtt aaagattttg attttgataa tggctgcatc actgtgcatg acggtaaggg
     7621 tgggaaaagc agaaacagcc tactgcccac gcgcctaatc ccagcaataa aataactcat
     7681 tgagcaagcg cggcttattc agcaagacga caacttacaa ggcgtagggc catcgctgcc
     7741 ttttgcttta gatcacaaat acccttctgc ttatcgacaa gcggcgtgga tgtttgtctt
     7801 tccctccagc acgctctgca accacccgta taacggcaaa ttatgccgcc atcatctgca
     7861 tgactccgtt gcgcgaaagg cattgaaggc agccgtacaa aaagcaggca tcgttagcaa
     7921 gcgtgtcact tgtcatacat ttcgtcactc gtttgctacg catctattac aagcggggcg
     7981 tgatattcgc actgtgcaag aactcttagg gcataacgat gttaagacca cgcaaatcta
     8041 tacgcatgtg ttgggtcagc attttgccgg caccaccagt cctgcggatg gactgatgct
     8101 acttatcaat cagtaaaagt agataaatat ctttatgtag ccctgagtag cccctgatac
     8161 tttttatggt gaaaaagata aacaatcata gaggggataa atatgagcca gttctaccat
     8221 tgttcagata agtttaagca agatactgtc aatcacagtg accattcatg gtcattacac
     8281 ttcctccagt tgccagacaa tgcctaacat aaaaacgcaa aagcccaaaa gtcagagctt
     8341 aggtgttcta ggcgttttct aagcgctgaa gaaatacgtc ttaccgtaga ttgcgtgcgt
     8401 ttctgaagca cattttcctt atacatggct ttatcccagg cagcttcatc aagctcttgg
     8461 agtaacaagc cagtaataat gcgacactct cgggtttgta gcgagccgct ggtgagatcg
     8521 gagttatagc ggaaaggggt catttgtaat tatttgtggg tggaggatag ttaacttatt
     8581 attgtagttg gaagaggctg ctttccaaac attagcacct cgtaatgctt gttactgtat
     8641 attaatttat gctcttagcg catgttaatg ggctattaca atgcataatg tccacatcaa
     8701 gataactgtg gcttaacacc tgtacaagcg aaaaaaatca atcacctgtc cggaaatacg
     8761 ggatcactgt aaagtgttgg tgaggcagtc taggaggggg atttaatttg ggatactgat
     8821 gaaaggatta tgatcagttg gatataggat cgtactaagc tgagcggcag gttagctaga
     8881 ttagttaacc agataagtga aatctagttc caaactattt tgtcattttt aattttcgta
     8941 ttagcttacg acgctacacc cagttcccat ctattttgtc actcttccct aaataatcct
     9001 taaaaactcc atttccaccc ctcccagttc ccaactattt tgtccgccca cagggctgcc
     9061 g
//
LOCUS       DAJTQY010000131         9014 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.131, whole genome shotgun sequence.
ACCESSION   DAJTQY010000131 DAJTQY010000000
VERSION     DAJTQY010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9014
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.131"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(97..1473)
                     /gene="trkA"
                     /locus_tag="OO349_003527"
     CDS             complement(97..1473)
                     /gene="trkA"
                     /locus_tag="OO349_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HCR5868465.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(1495..2784)
                     /gene="rsmB"
                     /locus_tag="OO349_003528"
     CDS             complement(1495..2784)
                     /gene="rsmB"
                     /locus_tag="OO349_003528"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HCR5868466.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRRQEELLAEFNTRDARYLHPSWLLKRLQKAYPEQ
                     WQSIAEANNQRPPMWLRINRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRIYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNQPGAEEGDGFFYAKLIKK"
     gene            complement(2830..3777)
                     /gene="fmt"
                     /locus_tag="OO349_003529"
     CDS             complement(2830..3777)
                     /gene="fmt"
                     /locus_tag="OO349_003529"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HCR5868467.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPIKVLAEEEGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINIHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(3792..4301)
                     /gene="def"
                     /locus_tag="OO349_003530"
     CDS             complement(3792..4301)
                     /gene="def"
                     /locus_tag="OO349_003530"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCR5868468.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            4449..5573
                     /gene="dprA"
                     /locus_tag="OO349_003531"
     CDS             4449..5573
                     /gene="dprA"
                     /locus_tag="OO349_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HCR5868469.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLQATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATCGVTITSGLARGIDGVVHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLFEQGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            5545..6018
                     /locus_tag="OO349_003532"
     CDS             5545..6018
                     /locus_tag="OO349_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HCR5868470.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            6047..6589
                     /locus_tag="OO349_003533"
     CDS             6047..6589
                     /locus_tag="OO349_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HCR5868471.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPVCGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            6594..7166
                     /gene="tsaC"
                     /locus_tag="OO349_003534"
     CDS             6594..7166
                     /gene="tsaC"
                     /locus_tag="OO349_003534"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HCR5868472.1"
                     /translation="MNNNLQGDAIAAAIDVLNEKRVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDVQRETIFSCWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYDKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            7171..7989
                     /gene="aroE"
                     /locus_tag="OO349_003535"
     CDS             7171..7989
                     /gene="aroE"
                     /locus_tag="OO349_003535"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HCR5868473.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNVFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            7986..8243
                     /locus_tag="OO349_003536"
     CDS             7986..8243
                     /locus_tag="OO349_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HCR5868474.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(8219..8773)
                     /locus_tag="OO349_003537"
     CDS             complement(8219..8773)
                     /locus_tag="OO349_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HCR5868475.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         2257 a         2188 c         2247 g         2322 t
ORIGIN      
        1 ctaacaaatg ttaagccatt ttcctgccag gaatgaattt aattaatcct tgataatcaa
       61 atggaaataa taaggcgtca ttagacgcct tattaattac aagaagaaag ggcttggctg
      121 gaagagtctt tcgacgtcgg taataaactt tttatctgtg aggaacataa ttacgtgatc
      181 gccttgctca atgcgcagat tgtcattggc aatcatcacg tcgtttccac gtaccaccgc
      241 tccaataatc gttcctggcg gtagcttgat ttcgtcaatg actctgccga caacgcgtga
      301 ggtgctttca tcaccgtgag caacggcttc aatagcttct gctacgccgc ggcgcaatga
      361 ggaaacacca acaatatctg cttttcgcac atggctaagc aacgcagaaa tagttgcttg
      421 ttgtggtgaa attgcaatat cgatcacgct cccctgaacc agatccacat aagcgcgacg
      481 ctggatcaat accatcacct ttttcgcacc catacgtttg gcaagcatgg cggacatgat
      541 attggcctcg tcatcgttgg tgacggcaat aaacagatca acttgatcga tatgttcttc
      601 ggccagcagt tcttgatccg acgcatcacc aaaaaagacg atcgtattct gcaacttttc
      661 cgccagttcg gcagcgcgct gctgattacg ttcgatgagt ttaacgctgt aatctttttc
      721 cagacgacgc gccagccctg caccgatatt accgccacca accagcatga tccgtttata
      781 cggtttttcc agtcgctgta attcactcat caccgcgcgg atatgctgtg aagcggcaat
      841 aaagaacact tcatcaccag cttcaacaat ggtcgaacct tgtggacgaa tggggcgatc
      901 gtggcggaaa attgctgcca cacgagtatc gatatgtggc atatgttcgc gcatggtcga
      961 aagtgcatta ccaatcagcg ggccgccata ataggcttta actacagcga ggctgacttt
     1021 accctcagcg aagttcacca cctgcaatgc gcctggatac tcaatcagtc ggtaaatatt
     1081 atcgataacc aactgctctg gtgcgatcag atgatcaatc ggtacagcat ctgaatgaaa
     1141 tagcttatcg gcatcgcgta cgtagtctgg tgagcggatg cgagcgatgc gattaggggt
     1201 gttgaaaagt gagtaggcta cctggcaggc aaccatattg gtttcatctg aactggttac
     1261 agcaaccagc atatcggcgt cgtcggcacc tgcctcccgc aatacgcgtg gatgagagcc
     1321 atgcccctgc acgactcgca ggtcaaattt atcctgcaag gtccgcagac gctcaccgtt
     1381 ggtatcgaca acagtaatat cgttgttctc gccaaccagg ttttccgcca gtgtgccgcc
     1441 aacctggccg gcacccagaa tgataatttt catcagtcgc gacccgttat ctcatcactt
     1501 tttgattagc ttagcgtaaa agaagccgtc gccctcttcg gcaccaggct gattttgttt
     1561 acccggttgc tctggtgttc ctgtttcgca aagttcggca tcagcggtac gttgcaaaaa
     1621 ggctttaatc tgcaggctat tctcttccgg taacaccgaa caggtggcat agaccagggt
     1681 tccaccggat tttaaatgcg gccaaatggc gtcgagaatt tcagactgca attgcgcgag
     1741 ttccgggata tcgcgatcgc gacgtaacca tttaatatct ggatggcgac gaatcacccc
     1801 cgttgctgaa caaggcgcat ctaataaaat gcgatcaaac tgttgctcgc cacaccattg
     1861 ggaagggtaa cggccatcac cttgtttcac ggttgccttc ataccaaggc gttttaaatt
     1921 gtcgtaaatg cgagagaggc gctgttcgtc aatatcaacc gcaacaacct gcgcttctgg
     1981 tgccacctca aggatatgcg ttgttttacc gccgggggcg gcacaaagat ccaagatgtg
     2041 ttcaccgttt tgtggcgcaa gccaggtcat gcaaccttgt gctgatgcat cctgaacggt
     2101 aacccatccg tcttcaaaac caggtagcgc atgaacaggt gcaggtgttt ccagccgtac
     2161 agcatcaggg taatccgcat gcgggaaacc tttcattcct gcttcatcca gcaatgcaag
     2221 ccagctgtcg cgggaatgat gcgtacgatt aatacgcagc cacattggcg gacgctggtt
     2281 attggcttcg gcgatggatt gccactgctc tggatacgct ttttgcagac gcttcagcaa
     2341 ccaggaagga tgcagataac gtgcatcacg ggtattaaac tcggctaata actcttcttg
     2401 ccgacgctgg aactggcgta atacaccgtt aatcaaccct ttaagttgcg gacgcttaat
     2461 tgcgatagcg ccttcaaccg tttcagccag cgcagcatga ggtggaatgc gggtataaag
     2521 cagttgatac aaaccaacca taatcaggta atgcacagtg cgctgtttgc cggtcatcgg
     2581 acgggccatt aacttattaa tcagccagtc taactgcgaa agcgtacgca gtacgccaaa
     2641 gcacaactct tgaagaagtg ctttgtcttt atcggaaact ttttgctgga gcggtggcag
     2701 aatgttgctt aatgattgcc cttgctcgac gacttgttca acggcctggg ccgccatgct
     2761 acgtaaatta cgttgttttt tcataagtat aaaaatgctc ggcaagaccg ggcttagaag
     2821 agtggactat cagaccagac ggttgcccgg aacaaaccat tcccgacgag agttcaggag
     2881 gtcttgcgcg ctcatcgctt tcttacccgc aggttgtaac gagagcaggt tcaggatgcc
     2941 atcaccagtc gcaacctgaa tgccttgttt gttggcttca aggatcgttc ctggtgcagc
     3001 gttggttgcc gtatcaatga ccgacgcttt ccagacttta accggctgtc cttcaatttc
     3061 cagccagctc attggccatg ggttgaaagc gcgaatgcag cgttcaagct gtgctgccga
     3121 aagtgaccag tcaatacgcg cttcttcttt actcaacttc tcggcgtaag tgacaagagt
     3181 ttcgtcctga acttctggtt tcgccgtgcc gtctgccaat tgtttcaacg tggtgataag
     3241 cccttgtggg ccaagctctg ccagcttgtc gtacagcgta ccactggtat cttctgcagt
     3301 aatcgggcag gagagcttat agagcatgtc accggtgtct aaaccaacat ccatttgcat
     3361 aatggttaca ccagtttctg catcacccgc ccatagtgag cgttggattg gtgcagcacc
     3421 gcgccagcgt ggcagcagtg aaccatgaat gttgatacag ccaagacgcg gcatctccag
     3481 cacagcttta ggcagaatta aaccataggc gacgacgacc ataacatcag cctgcaaatc
     3541 ggcgaccagt tgctggtttt cttgtggacg cagggaaaca ggttgaaaaa cgggcagacc
     3601 ttcttcctca gccagaactt taatcgggct gggcatcagt tttttaccgc gtcctgccgg
     3661 tcggtctggc tgggtgaaca cgccaacgac gttatgacca gaagacaaca gcgcgtcgag
     3721 atgacgcgct gcaaagtcag gtgtacccgc aaaaataata cgtagtgatt ctgacacgtt
     3781 agttcttatc cttaagcccg ggctttcaga cgatccagtt tttcaacttt ctgacgaatt
     3841 cgttgttgtt tcagcggtga cagataatcc ataaacagtt tgccaaccag gtgatccatc
     3901 tcatgctgaa tacagatagc taacagaccc tctgcttcca gttcaaatgg tttaccgtcg
     3961 cggtcaaggg cgcgaatttt aactttctct gcgcgcggca ctaaagcacg ttgttcaggg
     4021 atcgacaggc aaccttcttc aatgcctgtt tcgccgcttt tttctaaaag ctccggattg
     4081 attaacacca gccgttcgtc acggttttcc gaaacatcaa taacaatgat acgttgatgg
     4141 atatcaacct gggttgccgc caggccaata ccttcttctg cgtacatcgt ctcgaacata
     4201 tcatcgacga tacgctgaat ttctgcattc acttcttcta ccggtttagc aactttgcga
     4261 agccgctcgt ccggaatatg taacacttgc aaaactgaca taaatctcca gagatgtgtt
     4321 caggagttag aaagattatt tcttctattc tagacaaatc cccctctgat tgacagcatc
     4381 acttgattga cagcatcact gaccaatcgc aaagattgct aaggcttgct tatggcaggg
     4441 agataaggat ggtcgataca gaaatttggc tgcgtttaat gagcatcagc agcttgtacg
     4501 gcgatgatat ggtccgtatc gctcactggc tggcaaaaca gtcgcatatt gatgcggttg
     4561 tattgcagca aacagggctt acattgcggc aggcacaacg ctttctttca tttccacgaa
     4621 agagtatcga aagctcactt tgttggttgg agcaacccaa ccatcattta atccctgcgg
     4681 acagcgaatt ttatcctcct caacttcagg cgacgacaga ttaccccggc gcactgtttg
     4741 ttgaaggaga actgcacgcg ctgcattcat ttcagcttgc cgtagtgggg agtcgggcgc
     4801 attcatggta tggcgagcga tgggggcgat tattttgcga aactctggcg acgtgtggag
     4861 tgacaattac gagtggactg gcgcgtggaa tcgatggtgt ggtgcataaa gcggccttac
     4921 aggtaaatgg cgtcagcatt gctgtattgg ggaatggact taataccatt catccgcgtc
     4981 gccatgcccg actggctgcc agtctgtttg aacagggtgg cgctctcgtc tcggaatttc
     5041 ccctcgatgt tccacccctt gcttacaatt tcccacgaag aaatcgcatt atcagtggtc
     5101 taagtaaagg tgtactggtg gtggaagcgg ctttgcgcag tggttcgctg gtgacagcac
     5161 gttgtgcgct tgagcagggg cgtgaagttt ttgccttgcc aggtccaata gggaatccgg
     5221 gaagcgaagg gcctcactgg ttaataaaac aaggtgcgat tcttgtgacg gaaccggaag
     5281 aaattctgga aaacttgcaa tttggattgc actggttgcc agacgcccct gaaaattcat
     5341 tttattcacc agatcagcaa gacgtggcat tgccatttcc tgagctcctg gctaacgtag
     5401 gagatgaggt aacacctgtt gacgtcgtcg ctgaacgtgc cggccaacct gtgccagagg
     5461 tagttactca actactcgaa ctggagttag caggatggat cgcagctgta cccggcggct
     5521 atgtccgatt gaggagggca tgccatgttc gacgtactaa tgtatttgtt tgaaacctat
     5581 attcacactg aagctgagtt gcgtgtggat caagacaaac ttgaacagga tcttaccgac
     5641 gcaggttttg agcgagaaga tatctacaat gccctgctat ggctggaaaa acttgctgat
     5701 tatcaggaag ggttggcaga accgatgcaa ctggcctcgg atcctctctc catgcgtatt
     5761 tatacaccgg aagagtgtga acgactggat gccagctgcc gtgggtttct gcttttcctt
     5821 gagcagattc aggtgctcaa ccttgaaact cgtgaaatgg tgatagagcg agtgctcgcg
     5881 ctggataacg cagagttcga actggatgat ctgaaatggg tgatcctgat ggtgttgttc
     5941 aacatcccgg gctgcgaaaa tgcgtaccag caaatggaag aattactctt tgaagtgaat
     6001 gaaggtatgc tgcattaatc atttctttaa ttcagcataa gttgttatgg caaaatcagc
     6061 actgttcacg gtgcgtaata atgagtcctg cccaaagtgc ggggctgaac tggttattcg
     6121 atccgggaaa cacggtccgt ttcttggatg ctcacagtat ccggcgtgtg actacgtccg
     6181 tcctctgaaa tcttcagcgg atggacatat tgtcaaagtt ctggaggggc aggtttgccc
     6241 cgtatgtggc gcaaatctgg tattacgcca gggacgcttt ggtatgttta ttggttgcag
     6301 taactaccct gaatgcgaac ataccgaact tatcgataaa ccggacgaaa cagcaattac
     6361 atgcccccaa tgtcggacgg gccatctggt ccagcgccgc tcccgttatg gcaaaacatt
     6421 tcactcctgt gatcgctacc cggagtgtca atttgccatt aacttcaaac caatagctgg
     6481 agaatgccct gagtgtcatt atccgctact catcgaaaag aaaaccgcgc agggtgtaaa
     6541 acacttttgt gccagtaaac aatgtggaaa gccggtttcg gcggaataat aacgtgaata
     6601 ataacctgca aggagacgct atcgcagctg cgatagatgt cctcaatgaa aaacgtgtca
     6661 tcgcctatcc aacggaagcc gttttcggtg tcgggtgcga tcctgatagc gaaacagcag
     6721 tgatgagact gttggagtta aaacagcgtc cggttgataa ggggctgatt ttaatcgcgg
     6781 caaattacga gcagcttaaa ccctatattg atgacaccat gttgactgac gtgcagcgtg
     6841 aaaccatttt ttcctgctgg ccaggtcctg tcacctttgt ctttcccgcg cctgcgacaa
     6901 caccgcgctg gttgacgggc cgctttgatt cgcttgctgt acgagtcacc gaccatccgt
     6961 tggtggttgc tttgtgccag gcttatgata aaccgctggt ttctaccagt gccaacttga
     7021 gtggattgcc accttgtcga acagtagacg aagttcgcgc acaatttggc gcggcgttcc
     7081 cggttgtgcc tggtgaaacg ggggggcgtt taaatccttc agaaatccgc gatgccctga
     7141 cgggtgaact gtttcgacag gggtaacata atggaaacct atgctgtttt tggtaatccg
     7201 atagcccaca gcaaatcgcc attcattcat cagcaatttg ctcagcaact gaatattgaa
     7261 catccctatg ggcgcgtgtt ggcacccatc aatgatttca tcaacacact gaacgttttc
     7321 tttcgtgctg gtggtaaagg tgcgaatgtg acggtgcctt ttaaagaaga ggcttttgcc
     7381 agagcggatg agcttactga acgggcagcg ttggctggtg cagttaatac cctcaagcgg
     7441 ttagaagatg gacgcctgct gggtgacaat accgatggtg taggcttgtt aagcgatctg
     7501 gaacgtctgt cttttatccg ccctggttta cgtattctgc ttatcggcgc tggtggagca
     7561 tctcgcggcg tactactgcc acttctttcc ctggactgtg cggtgacaat cactaatcgg
     7621 acggtatccc gcgcggaaga gttggctaaa ttgtttgcgc acactggcag tattcaggcg
     7681 ttgggtatgg acgaactgga aggtcatgag tttgatctca ttattaatgc aacatccagt
     7741 ggcatcagtg gtgatattcc ggcgatcccg tcatcgctca ttcatccagg catttattgc
     7801 tatgacatgt tctatcagaa aggaaaaact ccttttctgg catggtgtga gcagcgaggc
     7861 tcaaagcgta atgctgatgg tttaggaatg ctggtggcac aggcggctca tgcctttctt
     7921 ctctggcacg gtgttctgcc tgacgtagaa ccagttataa agcaattgca ggaggaattg
     7981 tccgcgtgaa tcaggccatc cagtttccgg acagggaaga gtgggacgag aataaaaaat
     8041 gtgtatgttt tcccgctctc gtgaatggta tgcaactgac atgcgcgatc tctggcgaga
     8101 gtctggcgta tcgctttact ggagatacgc cagaacagtg gttagcgagt tttcgtcagc
     8161 atcgctggga cctggaagaa gaagcggaaa acttaattca ggaacaaagt gaagatgatc
     8221 aaggctgggt ctggttaccc tgatccagat attcgtcctt ccatttcacg taattattcg
     8281 cggaatagcg taacccagcc ttctcttcat cacttaacgg gcggatctgt ttgacggggc
     8341 taccgagata cagatatccg ctctccagcc gtttattttg tgggaccaga ctacccgcac
     8401 caatcatcac atcatcttct actattgcgc catcaagtaa aattgagccc atcccaacca
     8461 aaactcgatt gccaatggtg cagccgtgga gcatcacctt gtgaccaaca gtgacatctt
     8521 cgccaatggt taatgggttg ccatctgggt tgtacgagga tttatgagtg acatgcaaca
     8581 tactgccatc ctggatattg gtgcgtgctc cgatctgtac ataatgtaca tctccacgaa
     8641 tcacaacgag cggccagatc cccacatcat cagccagacg aacgtcacca atcacgacac
     8701 tgctatcgtc gatcattacg cgctgaccga tttgtggaaa aagatcgcgg tatgggcgta
     8761 aaacatcaga catacttacc tcagcaataa atgatttact aatgactttg ggggcattat
     8821 tggccttgtg caagtctttt agtatgcaaa aaagcaccgt tttgtgtgcg attgcagcaa
     8881 aaagggtgaa aaaacaacaa acagaaaaaa agatcaaaaa aatacttgtg caaaatattg
     8941 ggatccctat aatgcgcctc cgttgagacg acaacgtgaa acacttcaca ggatggtcgg
     9001 aacaacgaag agaa
//
LOCUS       DAJTQY010000132         8989 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.132, whole genome shotgun sequence.
ACCESSION   DAJTQY010000132 DAJTQY010000000
VERSION     DAJTQY010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8989
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.132"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            146..2330
                     /gene="espL4"
                     /locus_tag="OO349_003538"
                     /pseudo
     CDS             146..2330
                     /gene="espL4"
                     /locus_tag="OO349_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
     gene            complement(2299..4036)
                     /gene="aceK"
                     /locus_tag="OO349_003539"
                     /pseudo
     CDS             complement(2299..4036)
                     /gene="aceK"
                     /locus_tag="OO349_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
     gene            complement(4220..5523)
                     /gene="aceA"
                     /locus_tag="OO349_003540"
                     /pseudo
     CDS             complement(4220..5523)
                     /gene="aceA"
                     /locus_tag="OO349_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
     gene            complement(5553..7154)
                     /gene="aceB"
                     /locus_tag="OO349_003541"
                     /pseudo
     CDS             complement(5553..7154)
                     /gene="aceB"
                     /locus_tag="OO349_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
     gene            complement(7423..8352)
                     /gene="metA"
                     /locus_tag="OO349_003542"
     CDS             complement(7423..8352)
                     /gene="metA"
                     /locus_tag="OO349_003542"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HCR5868476.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYLRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            8509..8952
                     /locus_tag="OO349_003543"
     CDS             8509..8952
                     /locus_tag="OO349_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCR5868477.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNEREQPVGFMLLSGQHMDALFIDPDVCGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
BASE COUNT         2356 a         2168 c         2167 g         2298 t
ORIGIN      
        1 cgcaagatct cattccaccg aaacttttac ggctgcatta tgagattgag cgatttatga
       61 tattttttta gttgtaatca tattaaattt tttcactgtt ctgatagtta aaattcaaga
      121 catcaataaa caatgagata tttaaatgat tactcgtatt cctcgtagtt ctttctctgc
      181 aaatattaat aatacagccc agacaaatga acaccaaacc ctgagtgaat tgttttataa
      241 agaactcgag gataaatttt ctggcaagga gctggcgacg cctctattaa aaagcttctc
      301 agagaactgt agacaaaatg ttcgtcatat ctttagcaac aaggattttg tcattaaatt
      361 ttccacgtcc gtcttacaag ctgataagaa agaaattacg ataattaata aaaacgaaaa
      421 cacgacactc actcaaacca ttgctacaat atttgaagaa tacctaatgg aaattttacc
      481 tcaacgctca gacactcttg ataaacaaga attaaaccta aaatcagata gaaaagaaaa
      541 agaattccca agaattaaac ttaatggtca atgttatttc ccggggcgcc ccaaaaccgt
      601 atagtatacc gacacattgc tgcacaatat attaatgata tttatcagaa tgttgattac
      661 aaaccccatc aagatgatta ctcttcagct gaaaaatttc tcactcactt caacaaaaaa
      721 tgcaaaaacc agactttggc gttgatttcc agccgtcctg aggggcgttg cgttgctgcc
      781 tgcggtgatt tcgggctagt tatgaaagca tattttgaca agatggaatc aaatggcatc
      841 agtgttatgg cagccatatt actggtggat aaccatgctt tgacggtccg gctaagaata
      901 aagaacacaa ctgaaggatg tacccattac gtggtttcgg tttatgatcc taatgtaact
      961 aacgataaaa taagaattat gagcgaaagc aaagaggata ttaaacacta ttctctgatg
     1021 gattttatga atgtagatta tagcctcctg aaatggtcaa atgatcatgt tattaaccaa
     1081 tctgttgcaa taattccagc acttccgaaa gaacagctat tgatgttaaa aggaactgtg
     1141 gatgaaataa cccctccatt atcaccagca acgatgaatt tgctaatggc aattggtcag
     1201 aatcaccaac tcacgcaact gatgattcag ctccagaaaa tgccagaact acatagaaca
     1261 gaaatgttga ctgcctataa tagtattaac ctccccggtt tatatttggc tataaattat
     1321 ggcaatgcgg atatcgttga gactattttc aattcattgt cagaaacagg atatgaaggg
     1381 ttactctcga aaaaaaatct catgcatatt ctggaggcaa aagataaaaa tggtttttct
     1441 ggattatttt tagcgatatc acgtaaggat aaaaatgttg taacctcgat tctgaacgtc
     1501 ttacctaaac tggccacaac acatcattta gataatgaac aggtgtataa attcctgagt
     1561 gccaaaaata gaacgtccag ccatgtttta taccatgtta tggcgaatgg tgatgccgac
     1621 atgctgaaaa tttttttggt cgcgttacct ttgttaattc gcacatgtca tttgactaaa
     1681 gaacaggtac tcgatctcct gaaggcaaag gatttttatg gttgcccaag actatacctg
     1741 gcgatgcaaa atggacatag cgatatcgtg aaagttattc tcgaagcatt gccctgtctg
     1801 gcccaggaaa ttaacatttc agcttccgat attgtagatc ttctgaccgc taaaagtctt
     1861 gcgcgcgaca cgggtttgtt tatggccatg cagcgcggac atatgaacgt tattaatact
     1921 atttttaacg cattacccac tctttttaat acgtttaaat tcgataaaaa aatatgaagc
     1981 ccctcctcct ggcaaataat tctaatgaat atcccggttt gttttcagcg atacagcata
     2041 aacaacaaaa cgttgtagag acggtttatc ttgctttatc tgaccatgca cgcctgtttg
     2101 gatttaccgc tgaagatatt atggattttt ggcaacacaa agcgccacaa aaatactctg
     2161 cctttgagtt ggcttttgaa tttggtcacc gggttattgc tgaattaatc cttaatacat
     2221 taaataagat ggctgaaagc tttggcttta cggataaccc tcgatacatt gcggagaaaa
     2281 attatatgga agctttactc aaaaaagcat ctccccatac cgtacgctaa atctttgcct
     2341 gcgccgatac gcataaacat cttccacatg cccttcacgt atgcggtttt gtagtgcgcg
     2401 ccagtaatca gcgcggaaca ggtcggcgtg catctcttca aacagcggac caatacgcgg
     2461 gtcggcgcat agccagtggc gaaactcttc cgggaaaaca tcgcccggcg agacgctgta
     2521 ccacggttcg ctggcaagtt cgtcttccgg atagcgcggc ggcgggatgt cgcggaaatt
     2581 cacttccgtc atgtagcaaa tttcatcgta atcataaaaa accacacgcc cgtgacgggt
     2641 gacaccgaag tttttaaaca gcatgtcgcc agggaaaatg ttagcagcgg caagctggcg
     2701 aatagcgtta ccgtattctt caatggcgtc gcgcaactgc tgaccttcca cttgttccag
     2761 ccagatattg agcggcacca tccgccgctc gattatagag atggcgaatt acaatctgct
     2821 caccaagatc ggtgattttt tccgctgctt cctggagcag taattccatt aataccgggg
     2881 aaatatgccg cttctccagc acaaagtttt caaactcctg ggtgtccgcc attcggccca
     2941 cacggtcgtg ctctttcacc agttgatagc aggcacgaac gtgagcggca gacatctctt
     3001 tctgcggcgc gaacttgtct ttgatgactt tgaatacccg atcaaagccc ggcagcgtaa
     3061 acaccaacat caccattcca cgaatacccg gcgcttcaat gaactgctca ttacagccct
     3121 gtagataaac gagatattcg cggtagcttt cggttttggc gtgcttctgg cagccgatag
     3181 ccatatacaa ttcagcagtg gttttacctg gcagaatttc ccgtagccac tcgaccagcg
     3241 ctgcgggcag cggtgcataa accataaaat aagaacgcgc aaagccaaaa acaatgctcg
     3301 cttcggcggt cgtcgtcagg caggtatcaa taaataactc gccgtcgtcc gtctggtgga
     3361 tcggcagtaa gaagggcaac gtgccggaag gcgtgatcag tttgcccacc agccaggcgg
     3421 ctttattgcg gtaaaacagt tcgttcgcca cctgtaaatg actttccgcg aggttgtctg
     3481 tccccagcgt ttccgtcaga tggcgaatga tgtaatgaat gtcacggctt ttattctgcc
     3541 agcgcaggcg cagcggcagg tcgctgataa cgcgcatcag tagagattcc cagccgtgat
     3601 cggggtgaaa gtctttcgcc agcgggcggg gaatggtacg aaagcggcgc tctggctgag
     3661 agctaaagat aaaaagccgc tcgggagtaa gcgagcggtg gtcaaataac cgacagtaca
     3721 cggagttaaa aaagctctcc gcaatctcga agcgcgggta atccggcaac agccgcgtgt
     3781 aatgctcttt aacgcgcagt aaaaatgccg cgtccgtact ttggccgtta gtaatgcagc
     3841 gcagttgctc cacgaccaga ccaacgtgat gatcgtaaag atggatacgg tttttcatcg
     3901 cctgctggac agcatgccag tcggcctgtt cgaaacgctg ctgcgccccg gaagtcactt
     3961 cgaggaatcg accatactga gcgtcgaagc cttgcaaaat ggtttgagca atcagtaatt
     4021 ccaggccacg cggcatttag cgccctcatc aggagcagag aattgccgga tgcggcgtaa
     4081 acgccttatc cagcctacgt tcggcaacgg ttgtaggcat gataagacgc gcaagcgtcg
     4141 catcaggcat cgtgcaccga ttgccggatg cggcgtaaat gccttatccg gcctacagtc
     4201 agcaacgttt gtaggttgct tagaactgcg attcttcagt ggagccagtc agcgcggtga
     4261 ctgaagacgt gccgccctga ataatagtcg tcactttatc gaagtaacct gtacccactt
     4321 cctgctggtg agatacgaag gtatagccat ctttcgcggc ggcaaattcc ggctgctgca
     4381 ctttctcaac atagtgcttc ataccttcgc cctgcgcata agcgtttgcc aggtcgaaca
     4441 tgttaaacca catgctgtgg atacctgcca gggtgatgaa ctggaacttg tagcccatat
     4501 ccgacagctg ctgctggaag ctggcaatag ttttgtcgtc gaggtttttc tgccagttga
     4561 acgacggcga gcagttataa gccagcagtt tgcccggata tttcgcgtgg atagcttgtg
     4621 caaagcgacg cgccagttcc agatccggcg tggaggtttc acaccagacc aggtcagcat
     4681 atggcgcata cgccaggcca cggctgatcg cttgctcaat gcccgcatga gtacggaaga
     4741 agccttcact ggtacgctcg ccggtaataa attcgctgtc atacgggtcg caatctgagg
     4801 tgatcagatc cgccgcatca gcatcggtac gtgcaaccag cagggttgga acgcccgtca
     4861 cgtcagctgc cagacgcgcc gcgaccagtt tctgaatagc ttcctgagtt ggcactaaaa
     4921 ctttgccgcc catgtgaccg catttcttca ctgacgccag ctgatcttcg aagtgaactg
     4981 ccgcagcacc ggcttcaatc atcgctttca tcagttcaaa ggcattcagg acaccgccaa
     5041 aaccggcttc cgcatcggca acgatcggca ggaagtaatc gacatagcgc ggatcgcccg
     5101 gctcaatgcc cacggaccat tggatctgat cggcacgacg gaaggtgttg ttgatccgct
     5161 ccaccacagc tggcaccgag tttgccggat agagcgactg atccggatac atgctggccg
     5221 ccaggttagc gtccgccgct acctgccatc ccgacagata gactgcttca atacccgctt
     5281 tcgcctgttg cagcgcctga ccgccagtca gtgcaccgag gctgttgatg tagctttttt
     5341 cgactcaccg tgcagcagac gccacatttt cgctgcgccc agttgcgcca gcgtgcattc
     5401 aggattgact gaaccgcgta atttcaccac atcttccgca ctgtatgggc gagtaatgcc
     5461 ttcccaacgc ggttgagtcc actctttctg taattcttca atttgttgtg tacgggtttt
     5521 catgtgcaga tgctccatag ttatgtggtg gtttacgcta acaggcggta gcctggcagg
     5581 gtcaggaaat caattaactc atcggaagtg gtgatctgtt ccatcaagcg tgcggcatcg
     5641 tcaaaacggc cctgggagaa acgttcttcg cccagttcgc tggcaatgac tttcatctct
     5701 tcgcccagca tctggcggaa caaggctttg gtcaccggtt tgccattgct caacgttttt
     5761 tgatgatgga tccactgcca gatcgaggta cgggaaattt cagccgtcgc cgcatcttcc
     5821 atcaggccat aaatcggcac gcagccgttg ccggagatcc acgcttcgat gtactgcaca
     5881 gccacgcgaa tgttagcgcg cataccttct tcggtgcgtt caccatcaca aggtgccagc
     5941 agctgatcgg cagtaatcgg cgcgtcttgt tcgcgcaata cttcaagctg atttttacgg
     6001 gagccgagaa tgtcgttgaa taccgccatt gccgtgtccg caaggcctgg gtgagcgatc
     6061 catgtgccat cgtgaccgtt attggcttcc agcgctttat ccgcttttac tttgttgagc
     6121 acctggttat tacgctcttc atctttgctc ggaataaacg cggccatgcc gcccatcgca
     6181 aaagcaccgc gtttatggca ggttttaatc agcaggcgtg agtaagcatt caggaatggt
     6241 ttatccatcg tcacggcctg tctgtctggc aggacacgat cgggatagtt tttcaacgtt
     6301 ttgatatagc tgaagatgta atcccagcga ccgcagttca gaccaacaat atggtcgcgc
     6361 agcgcgtgaa ggatttcgtc catctggaac acggcgggca gcgtttcaat cagcaacgtc
     6421 gccttgatgg tgccgcgcgg cagattaaag cgatcttctg catagctgaa gacttcgctc
     6481 caccaggccg cttcctgcta ggactgggtt ttcggcagat agaaataggg accgctgccc
     6541 tttgccaaca gtgcctgata gttgtggaag aaatagagcg caaaatcaaa caggctaccg
     6601 gggattgcct caccacgcca ggtgacatgt ttttccggca agtgcagacc gcgtacccga
     6661 caaatcaaaa ccgctggatt gggcttgagc tgataaattt tgcctgcttc attggtatag
     6721 ctgatggtac cgttaaccgc atcgcgcagg ttaatttgcc cgtcgatcac tttgttccag
     6781 tctggtgcca gtgaatcttc gaaatcggcc ataaagactt tcacattggc gttcagtgcg
     6841 ttgatcacca tcttgcgctc taccgggcca gttatctcta cgcggcggtc ttctaagtcc
     6901 gcaggaatcc cgcgaatttt ccaatcagca tcgcgaatgg aagccgtttc cgaaataaaa
     6961 tcaggcaacg ttccgttatc aatatcttgc tgctgctgaa tgcgcgctgc cagaagttta
     7021 ttgcgttgtg gcgtaaaatg cgtcaccagc tcagtcagaa attctaccgc ttcggcagta
     7081 agaatttgct tctcctgctc gccatacggc cttgtgaaag ccagttcatc ggttgttgtt
     7141 gcctgttcag tcatcgtgca gctcctcgtc atggatccga aaacttcccc tctgtgaacg
     7201 aaggaacgtt gtgcgctttt cgttcagcac aactaagact actcatttaa aatgcaaaat
     7261 caaaaacaat ttccatttta attaaaaata cttgataact tattgataac aatatgaata
     7321 aaatttattc acttgctgag gtgcgtttcg gaaataaaaa aggcacccga aggtgcctga
     7381 ggtaaggtgc tgaatcgctt aacgatcgac tatcacagaa gattaatcca gcgttggatt
     7441 catgtgccgt agatcgtatg gcgtgatctg atagacgtaa tagttgagcc agttggtaaa
     7501 cagcaaatta ccgtgactac gccagctcgc tcgcggtttg ttttgcggat cgttgtgcgg
     7561 gaaatagtta tacggtacat ccgggtctag tccggcttcc acatcgcgta aatattcctg
     7621 cgccagcgtt tgcgcatcat attcgggatg gcccgtcaca aaggcaatgc gcttatcttt
     7681 gctggcaaac agatatgcat ccccttcttc cgtctctgcc agaatttcca gatcggtgta
     7741 atcacgaatc aacgctgccg gaaagtcagc atagcgcgaa tgcggtgcca ggaatgaatc
     7801 atcaaagcca cgcgtcagaa gcgcatgagg atggagaata tgatgctcgt aaacgccaga
     7861 gagtttgtcg gtgcgagttt gcttaggaat gccgtagagg atattgagcg cggcctgtac
     7921 cgcccagcag acaaacagcg tcgaggtgac gtgatctttc gaccactcca gcacctgttt
     7981 gatctgcggc cagtaagcga catcattaaa ctccaccagg cccagcggcg caccggttac
     8041 aatcagaccg tcaaagttct gttcctgaat atcttcaaag ttacagtaga agttgttcag
     8101 atgctctgcg ggcgtgttgc gcgattcacg ggaatcgatg cgcaacagct gaatatcgac
     8161 ctgcaaaggt gagtttgaaa gcaggcgcag aaactgattt tcagtttcaa tcttcttcgg
     8221 catcaggtta aggatcagaa ccttaagtgg acgaatttcc tgaccagacg cacgagaagt
     8281 tgtcatcaca aagacgtttt cttcacgcaa gaaattgacg gcgggtagct cgtccggcac
     8341 acgaatcggc ataacctgat tacctcacta catacgctta tacgtttaga catccagata
     8401 gctgaagata accagaaaat ttgccaatgt cgagcctgca tgttgaaggt gagaaagttt
     8461 caaggaaaag gctacgttag aatataagaa tgacgaaaag gagagaaaat ggttattagt
     8521 attcgccgct cacggcatga ggaaggggag gaactcgttg cgatttggtg tcgatctgtc
     8581 gatgccaccc acgatttttt atcagcagag tatcgggccg agctggaaga gctggtgcgt
     8641 tcctttctgc cggaagcacc gttgtgggtc gcggttaatg aacgggagca gccggttggg
     8701 tttatgttgt taagtgggca gcatatggat gcgctgttta tcgatcctga tgtgtgcggc
     8761 tgcggcgtgg gtcggatgct ggtggagcat gcgctctcga tggcaccgga actgacaacc
     8821 aacgttaatg agcaaaatga gcaggcggtt gggttctata agaaggtggg ttttaaggtt
     8881 acgggacgct ctgaggtgga cgatttgggg aaaccgtatc cgttgctgaa tctggcgtat
     8941 gtgggggaat aagccgcccc tgccgtgcct gcccaagctg gagaagacg
//
LOCUS       DAJTQY010000133         8897 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.133, whole genome shotgun sequence.
ACCESSION   DAJTQY010000133 DAJTQY010000000
VERSION     DAJTQY010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8897
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.133"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            1..2887
                     /locus_tag="OO349_003544"
                     /pseudo
     CDS             1..2887
                     /locus_tag="OO349_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            complement(2997..3845)
                     /gene="alkA"
                     /locus_tag="OO349_003545"
     CDS             complement(2997..3845)
                     /gene="alkA"
                     /locus_tag="OO349_003545"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HCR5868478.1"
                     /translation="MYILNWQPPYDWSWMLGFLAARAVSGVETVADSYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLLDLQCNPQIVNGALGKLGAAR
                     PGLRLPGSVDAFEQGVRAILGQLVSVAMAAKLTARVAQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGTLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFLGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            3979..5331
                     /gene="yegD"
                     /locus_tag="OO349_003546"
                     /pseudo
     CDS             3979..5331
                     /gene="yegD"
                     /locus_tag="OO349_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
     gene            complement(5343..7283)
                     /gene="yegI"
                     /locus_tag="OO349_003547"
                     /pseudo
     CDS             complement(5343..7283)
                     /gene="yegI"
                     /locus_tag="OO349_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
     gene            complement(7280..8041)
                     /locus_tag="OO349_003548"
     CDS             complement(7280..8041)
                     /locus_tag="OO349_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HCR5868479.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVAWLNDQQPLLVMFL
                     ADGAGSVSQGGEGAMLAVNEAMAYMSQKVQGGELGLNDILATDIVLTVRQRLFAEAEA
                     KELAVRDFACTFLGLISSANGTLIMQIGDGGVVVDFGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTERVHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(8038..8697)
                     /locus_tag="OO349_003549"
     CDS             complement(8038..8697)
                     /locus_tag="OO349_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCR5868480.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNTGL
                     VTFRDELLADSLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPIDEWQAAANKVFRGEEDKRFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
BASE COUNT         2174 a         2357 c         2319 g         2047 t
ORIGIN      
        1 tggttgtcct gctgacgccc ggagacgatc ctctcagggc atttttgata tgggtactgt
       61 cagaatccat cggcgctctg gcactggtgc cgctgggatt gttatttaaa ccacactatc
      121 tgctgcgcca tcgcaaccca cggttgcttt ttgagtcgct gctcacgtta gccatcacac
      181 tgacgttaag ctggctttcg atgctgtatc tgccgtggcc ttttactttc attattgtgc
      241 tgttgatgtg gagcgccgtg cgcctgccac gaatggaagc ctttttgatc ttccttacca
      301 cggtgatgat ggtgtcgctg atgatggccg cggatccctc cctgcttgct acgccgcgta
      361 cgtacctgat aagccatatg ccgtggctac cgtttttgct gatcctgctg cccgccaaca
      421 tcatgaccat ggtgatgtat gcctttcgtg cggaacgcaa acacatttcc gaaagcgaaa
      481 cccgctttcg gaacgcgatg gaatattccg ctatcggtat ggcgttagtg ggcaccgagg
      541 gacaatggct gcaatccaac aaagcactct gccagtttct cggttacagt caggaagagc
      601 tgcgcggact cacctttcag caactgacct ggccggagga tctcaataaa gatctccaac
      661 aggttgaaaa gctgataagc ggtgaaataa acacctattc aatggaaaaa cgctactaca
      721 accgcaatgg cgatgttgtc tgggcgttgc ttgccgtctc actggtgcgc cacacggatg
      781 gcacgccgct ctattttatc gctcagattg aagacattaa cgagctaaaa cgcaccgaac
      841 aggtgaatca gcaactgatg gagcgcatca cgctggctaa cgaagcgggc gggattggca
      901 tctgggagtg ggagctgaag ccgaatattt ttagctggga taagcggatg ttcgagctgt
      961 atgaaattcc tccgcatatc aaaccgaact ggcaggtgtg gtacgagtgc gtgctgccgg
     1021 aagatcgcca gcatgccgaa aaagtgattc gttgcaatca cgctcgccct ttaagctgga
     1081 atttcgcatt gccgtgaaag atggcatccg ccatatccgc gccctcgcca accgggtact
     1141 gaataaagaa ggcgaagtcg aacgcctgct cggcattaat atggatatga ccgaggttaa
     1201 acagcttaac gaggcattgt ttcaggaaaa agagcgcctg cacattacgc tggattccat
     1261 cggtgaagcc gtagtctgta ttgatatggc gatgaaaatt acctttatga atccagcggc
     1321 ggagaagatg agcggctgga cgcaggaaga agcattaggt gttccgctcc tgacggtgtt
     1381 gcatattact tttggcgaca acggaccatt aatggagaac atttacagtg ccgacacctc
     1441 acgttccgcg attgaacaag atgtggtgtt gcactgccgg aacggcggca gttacgacgt
     1501 gcattacagt attacgccgt taagtactct ggacggcagc aatataggct cggtactggt
     1561 gattcaggac gtcaccgaat cacgcaaaat gctgcgccag ctgagctaca gcgcctccca
     1621 tgatgcactg acgcatctcg ccaatcgcgc cagttttgag aagcaactgc gtatcctgct
     1681 gcaaacggta aacagtacac atcagcgaca tgccctggtg tttatcgatc ttgatcgctt
     1741 taaagcggtg aatgacagcg ccgggcatgc ggcgggtgac gctttactgc gcgaactggc
     1801 gtcgttgatg ctgagtatgc tgcgctccag cgacgtgctg gcgcgactcg gtggtgatga
     1861 atttggtcta ctgttgccag actgtaatat cgaaagtgcg cgttttatcg ctacacgcat
     1921 tatcagtgcc gtgaatgatt atcactttat atgggaaggc cgtgtgcatc gggtaggtgc
     1981 cagtgccggg attaccttga ttgatgacaa caatcatcag gcggcagaag tgatgtcgca
     2041 ggctgatatc gcctgttatg cctccaaaaa tggtggacgg ggccgggtga cggtttacga
     2101 accacagcaa gctgccgcac atagcgaacg ggcggcgatg tcgcttgatg aacagtggcg
     2161 gatgattaaa gagaatcagt tgatgatgat cgcccacggt gtcgcttcgc cgcggatccc
     2221 ggaagcgcgt aatttgtggc tgatttcact taagctctgg agttgcgaag gcgagattat
     2281 tgatgaacaa aaatttcgtc gtagcttcag cgatccggca cttagccatg ctcttgaccg
     2341 acgggtattc cacgaatttt tccagcaggc cgcaaaagcg gttgccagta aaggcataag
     2401 catcgccctc cccctttccg ttgccggttt gagtagcgcc acgctggtga atgatctgct
     2461 tgagcagctg gaaaatagcc ctctaccacc acggttatta catctgatta ttccggctga
     2521 agcgatttta gatcacgcag aaagcgtgca aaaactgcgg ctggcgggat gtcggatagt
     2581 gctcagccag gtgggccgcg atctgcaaat cttcaactcg ctgaaagcga atatggcaga
     2641 ttacctgcta cttgatggtg agttatgcgc caacgtgcag ggtaatttga tggatgagat
     2701 gctgattacg attattcagg ggcacgctca gcgactcggg atgaaaacca tcgccgggcc
     2761 agtcgtttta cccttagtga tggatacgct ttctggcatc ggcgtcgatc tgatttatgg
     2821 tgatgtgatt gccgatgccc aaccgctgga tttgctggtg aatagcagtt atttcgcgat
     2881 taactgagag agcgtttgcc cattgcctga tgcgatgcta acgcgtctta tcaggcctac
     2941 aaatcgctca ttccgcaggc aggataaggc gctcgcaccg cttccggcga ccaacgtcat
     3001 gcttcgtctg gttgccagcc ttccgtatac cagatatgca acagcgcata agaacgccag
     3061 ggcttccagc gctcggcata acggcggatt tgtgccggtg tcatccccag aaatcgctgt
     3121 ttaatcagat aatcatccgg cagaaaaaca tctttcgcct gccagccgcg caaggcgaaa
     3181 taattcgccg tccagcgccc gatacccgga aaagtttgca gcgttttcat cgcctgctcc
     3241 acatcgcccg gtattgtcat tggtaaggtg ccctccagcg ccgcatttgc cagatgaatc
     3301 agcgcctctg cccgtttcaa cggcatacct aacgctttta atgcctgcgg gtcggctgct
     3361 gccagccgct gaggcgtggg gaagcagata tactccggaa aatcatccag ccgttcgcca
     3421 taaagctgtg ccactctggc ggtcaatttt gccgccatcg ccacgctcac cagttggcct
     3481 aaaatcgccc gcacgccctg ctcaaaagca tcaacactgc cgggtaaacg caatccgggc
     3541 cgcgccgcgc ctaacttgcc caacgcaccg ttaacaatct gcgggttaca ttgcagatca
     3601 agcaggcggc tcattttcgc cagacactca gcggcaacgg gttctaaacc tgcacttaaa
     3661 tttatgtgca gagtatggcg ggctatatcc ggaatagcag tcaccacgcc gcgatattcg
     3721 cccactgcca gactacgggc ataataactg tccgcgaccg tttccacacc actcaccgca
     3781 cgggcggcga gaaatcccaa catccacgac cagtcatacg gcggctgcca gttcaggata
     3841 tacatcgcat ctccttattc atccgctttc agcataaacg ttattcagac gttacgcttt
     3901 gctttcatat tccggttgtc gcgacggcaa catttcgcta aagtcacgcc ccttcttcac
     3961 cggcatgggg attatttcgt gtttattggt tttgattacg gtacagcaaa ctgttcagtg
     4021 gcggtcatgc gtgacggtaa accgcatttg ctaaaaatgg aaaacgacag cacgctgctg
     4081 ccttcaatgc tttgcgcgcc aacgcgtgaa gcggtaagcg aatggctgta ccgccatcat
     4141 gacgttccgg cagacgacga tgaaacgcag gcgttgttac gacgggcgat tcgttataac
     4201 cgcgaagaag atatcgatgt tacagcgaaa agcgtgcagt tcggtctttc ctcactggca
     4261 cagtacattg atgatccaga agaagtgtgg tttgtgaaat caccaaaatc gttcctcggt
     4321 gccagcggct taaaaccgca gcaggtagcg ctgtttgagg atctggtctg cgcaatgatg
     4381 ttgcacattc gccaacaggc gcaggcgcag ctgccagaag cgattactca ggcggtgatt
     4441 ggtcgcccga tcaacttcca ggggctgggc ggcgatgaag caaacgccca ggcgcagggg
     4501 attctggaac gcgcggcgaa gcgtgccggg tttaaggatg tggtattcca gtacgagccg
     4561 gtagcggctg ggctggatta cgaagccacc ttgcaggaag aaaaacgggt gctggtggtg
     4621 gatatcggcg gtggtacgac tgactgttca ttgctgctga tggggccgca gtggcgttcg
     4681 cgtctcgatc gtgaagccag tctgctgggt cacagtggtt atcgtattgg cggtaacgat
     4741 ctggatatcg ccctagcgtt taaaaacctg atgccactgc tgggtatggg tggcgaaacc
     4801 gaaaaaggca tcgccctgcc gatcctgccg tggtagaatg cggttgccat caacgacgtt
     4861 cctgcacaga gtgatttcta cagtagtgcc aacggtcgtc tgcttaacga tctggtacgc
     4921 gatgcccgcg aaccggaaaa agtggccctg ttacagaaag tctggcgtca gcgtttaagc
     4981 tatcgcctgg tgcgtagcgc agaagagtgc aaaattgctc tttcaagcgt agcggaaacc
     5041 cgcgcctcac tgccgtttat cagcaatgaa ctggctacgc tgattagcca gcgagggctg
     5101 gaaagcgccc tcagccagcc actggcgcgg attctggaac aggtgcaact ggccctggat
     5161 aacgcccagg aaaaaccgga cgttatctac ctgaccggcg gtagcgcccg ttctccgctg
     5221 attaaaaaag cgctggcaga acagttgccg ggcattccga ttgcaggcgg cgatgacttt
     5281 ggctccgtca ccgccgggct ggcacgctgg gcggaagtgg tatttcgtta aaagaaaatg
     5341 gctcagcagc ggcttaaatc agcctgcgcc tgagccagtt tttctgccgc ctgacgtaac
     5401 ggttccatca acggcatggt ccgtgctgga gcatgaaggc ggaatcgctg caactctgtc
     5461 gcgccgacag tcaacgtcga ttccagccga tgtcgtttgg cggccatttt cgccattacc
     5521 gcctgtctgt ctgctggcgt taccgcttcg ttcggcctga acacaaaacg gcgttcacag
     5581 ctcgctttcc agtcgatgac cgcctgggtc agatgatcac caaacccttt aacttgctta
     5641 acgccacgac gggtaacacc tgctgctgtt tcaataccaa aagaccataa cgccgcttta
     5701 cgcgcagggc caacgccggg aatagaggca acatcaataa aaaatccctc cagaaacttc
     5761 tacttctgcc gttcccttgc agtgtcatga agtgctgcca gagcgagttt cttttcttcc
     5821 ggtaacccga gaatttcgtc cttcattttt tcgagcatcg tccgtttggc gataaaacct
     5881 tccaggccgc ctacctgctg gatctggcgg actaaatggt catagtccat tttcgcacga
     5941 ttaaatgcct cacggcgttg ctgaacttct gctttgtacg ctttgcttgt cagactgcca
     6001 ataatccaga tagccgccag cacaggaacc aaaataatat aacgcggttc tgcctgaagg
     6061 ccgcaaagca gcagcgataa tgctgacagt gcgatctcaa gcagaatgat gtattcgcgc
     6121 cgtaacaggc ctaaaggaag cggcctgcca gtcggttgga aatgatcggg taatggcagt
     6181 tgcaatgctg gcggtgctac tgacgccatc accatcgccc agacttttgc cagcacaaaa
     6241 tccccgccgg tggtaatgac ctcttcgccg agatcaataa aatagataac gccttgatta
     6301 tccagcgtac accacgggca gtcggtcaaa tgagcgggat aaacatgcat tgccgaaacg
     6361 ggacatttct ttaactgttg gcgcagagaa tcaagtgccg ctacccacgc tttagccgtc
     6421 ggacgcccgg ttgccacgcc actttccgtg aatgcctgct gaaacatggc ttcaacatca
     6481 ctcggtaaca tcgatagcgg aatcgatcgt ggcggcggtt ttaaaccacg tcgctgatta
     6541 tctgacgcgt aggcataacg gaaatgggta atatccgtct ccagcgcatt acccgcatca
     6601 gagataagcg gcacaccaga ataaggatgc cggccaccaa acaagacgtg aaaaatcagc
     6661 aacgcaaggc caaaattacc gtgattttct gtgcgttcaa agccgacaaa tgacgacaag
     6721 gtttgtagct ctggcggcgt aaaatgcgac acgccgactt cgcataaatg cagtgtgcca
     6781 ttggcgttaa tctgaaagga gtcgctatca atcaacacca ctttgctgtc gcgacctacc
     6841 ataaaactgt tctggtttac gtcacccacg acatgcccgt gctcgtgaac cgtagcaaaa
     6901 gatgaagcaa tattgcgcgc aacatagagt agaaaatccc acgcacaatg agggtaacgt
     6961 tgacgacgat gtgccgggct atagatcata tgaatcggtt ctttaccaga aacttttggc
     7021 atcataaaac cgataacttt tccgcctcgt ccaccgtgaa gcgttgcctg cggccaggcg
     7081 acataattca acaattgcgc gtcagctgtc gcagccataa aggcaagttt gtcctgtttt
     7141 aaggcgggtg gcggcgtgtg ataaatcttg gcgacgctat cgacaaactc attgatatca
     7201 taaaccgcgc cttcaccgcc tttgcccagt tcacggcctg gcgtaacgca ctcaccagta
     7261 gcagtatata aagtgggttt catggtaacc acagcgccag tgccaacgtt ttatcatcat
     7321 ccgtacgctc gtttaccgcc gggctggaca aaaactgctt taacaattca ggtaataaat
     7381 cgagttgttc ctgcgttgct gacgccagtc cattgaaaaa cggggtaaag aaaggcacat
     7441 gcggagaatt atccaacata tttaacgcca gtcgctggat accatccgta aatgctgcaa
     7501 ctttatgcac acgctcagtg ctggtgaaag tttccagtct ggaaacggca tcttcatcgg
     7561 taatgaagtg cgtcatattg gcatattcac ccaccatcgg cgtaagcggc aattgcagac
     7621 cgtgaccaaa atccaccacc acgccgccat cgccaatctg cataatcaag gtgccattag
     7681 ccgatgaaat caatcccaga aacgtgcagg caaaatcgcg aactgctaat tctttggctt
     7741 ccgcctcggc aaaaagtcgc tgacgaacgg tcagcacaat atccgtggcg aggatatcat
     7801 taagccccaa ttccccaccc tgcacttttt gcgacatata agccatcgct tcattgacgg
     7861 cgagcatcgc gccttcgccc ccctgcgaaa cgctacctgc gccatcagca aggaacatca
     7921 ccagtaatgg ctgctggtca tttagccagg caacctgcat ctggcaggca tcctggcaag
     7981 gcaagtccgc gctgatgtgc gacgttccaa cagcggatgc ataaaccaga cgccagctca
     8041 cactgacgtc cagccttttg gcgcttccag cacaacttcc gttcccggcg tggagcggga
     8101 aaccgaacgc agcgaactgg ataaccagct aaacagttca cggaattgta atccttgcag
     8161 aggcaaaggc tgacgaacgc tgatttgtgc cagagtcttc atatccgccc cctgaacacc
     8221 gatggaaaag aaagcaaacc ttttatcttc ttccccccga aacactttgt tggctgcagc
     8281 ctgccactca tcgattggtg caccatcggt aatcaggaaa atccacgggc gataatacga
     8341 aataccattt gcacgatact cacgtttccg ctcctctacc atatccaggg ctttggtaat
     8401 ggccgcaccc attggtgtat cgccctgggc aaacaggatg ggcggaaaaa aattagcggc
     8461 gctggtaaag ggttgttcca catgcaccgg gccgaacgtc acaatgccga gttccactct
     8521 ttttaaagcc agcgaatcgg caagcagctc atcgcgaaag gtaaccaatc cggtgttaag
     8581 ttcattaata ggtctgccac tcatcgagcc agaaacatcc agcagcagaa tgcaggggca
     8641 acgtggttcc gggttactgg caaaatcgct ggtggcaaac gtgatttgtt cgctcataag
     8701 aaatcctttc ttagagaatt attgaggtcc ggtatcatac cgactgcaat aatgaaataa
     8761 gagcaaacga agaaactttc agaattagtc ccggcggcgc agaaaatttt gctattcgcc
     8821 tcgcaagaac ggagagccgt cgattgacac taagggcgga gtgacataat ttcaggagtg
     8881 agggttaggg agaggtt
//
LOCUS       DAJTQY010000134         8676 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.134, whole genome shotgun sequence.
ACCESSION   DAJTQY010000134 DAJTQY010000000
VERSION     DAJTQY010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8676
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.134"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            <1..305
                     /locus_tag="OO349_003550"
     CDS             <1..305
                     /locus_tag="OO349_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HCR5868481.1"
                     /translation="LIARWELTAEQAVAQQLKNQVSKGNLIDTGFCIFALSKLAMALS
                     STLDSIPLSMQRQFPDLTPRHIDHLKILIAKGANQCARAGDKLPDLLDEYIRTTTE"
     gene            277..2205
                     /locus_tag="OO349_003551"
     CDS             277..2205
                     /locus_tag="OO349_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HCR5868482.1"
                     /translation="MNISEQQLSNMMVAVTIALQPLVRVLPVTAVEWADQNYYLPKES
                     SYGEGEWKTLPFQVAIMNCMGNDEIRTVNLIKSARVGYTKMVLGVIGYFIEHKSRNTL
                     LFQPTDSAAEDFMKSHVEATIRDVPCLKKLSPWLGRKHRDNTLTLKRFSSGVGFWCLG
                     GAAAKNYREKSVDVVCYDELSSFEPDVEKEGSPTLLGDKRIEGSVWPKSIRGSTPKIK
                     GSCQIEKAANESAHFMRFYVPCPHCGEEQYLKFGDDATSFGLKWEKGKPETVYYLCEH
                     NGCVIRQSELDQTGGRWICDNTGMWTRDGLTFFSASGNEIPPPRSITFHIWTAYSPFT
                     TWVQIVCDWLDALKDPNGVKTFVNTTLGETWEEAVGEKLDHQVLMDKVVPYTATVPVR
                     VVYLTAGIDSQRNRFEMYVWGWAPGEEAFLVDKIIIMGRPDEEETLLRVDAAINKKYR
                     HADGTEMTISRVCWDIGGIDGEIVYQRSKKHGVFRVLPVKGASVYGKPVITMPKTRNQ
                     RGVYLCEVGTDTAKEILYARMKADPTPVNEATSYAIRFPDNPEIFSQTEAQQLVAEEL
                     VEKWEKGKMRLLWDNKKRRNEALDCLVYAYAALRVSVQRWQLDLAVLAASRKDATAQP
                     TLEELAAKLSGGVNGYSR"
     gene            2189..2392
                     /locus_tag="OO349_003552"
     CDS             2189..2392
                     /locus_tag="OO349_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5868483.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLNELN
                     RAINDAESVLGTTRRRRPLGVRL"
     gene            2389..3982
                     /locus_tag="OO349_003553"
                     /pseudo
     CDS             2389..3982
                     /locus_tag="OO349_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            3972..5477
                     /locus_tag="OO349_003554"
     CDS             3972..5477
                     /locus_tag="OO349_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HCR5868484.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSVPQ
                     QQVQLDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADTQVRGVLLDIDSPGGQATGAFDCADMIYRLRQLKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMGHVSYAGHLAQAGVDITLIYAGSHKVDGN
                     QFESLPAKVRQDMQQRIDAARRMFAEKVAMYTGLSVDAVMGTEAAVFEGQSGIEAELA
                     DELINASDAISVMAATLNTHDTGGTMPQLTATEAAAQENQRVMGILTCQEAKGREQLA
                     TMLAGQQGMSVEQARAILAVAAPQQSGVSAQSEADRIMACEEAKGREQLAATLAAMPE
                     MTVEKARPILAASPQANAGPSLRDQIMALDEAKGAEAQAEKLAACPGMTVENARAVLA
                     AASGRAEPVSASTTAMFEHFMANHSPAAVQGGVPQTSADGDADVKMLMAMP"
     gene            5514..5861
                     /locus_tag="OO349_003555"
     CDS             5514..5861
                     /locus_tag="OO349_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HCR5868485.1"
                     /translation="MVTKTITEQRAEVRIFAGNDPAHTATGSSGISSATPALTPLMLD
                     EDTGKLVVWDGQKAGHVVGILVLPLEGTETVLTYYKSGTFATEAIHWPERVDTHKKAN
                     AFAGSALSHAALP"
     gene            5919..6947
                     /locus_tag="OO349_003556"
     CDS             5919..6947
                     /locus_tag="OO349_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HCR5868486.1"
                     /translation="MGLFTTRQLLGYTEQKVKFRALFLELFFRRTVNFHTEEVMLDKI
                     TGKTPVAAYVSPVVEGKVLRHRGGETRVLRPGYVKPKHEFNYQQAVERLPGEDPAQLN
                     DPAYRRLRIITDNLKQEEHAIVQVEEMQAVNAVLYGKYTMEGEQFETVEVDFGRSVGN
                     NITQAGGTEWSAQDRDTFDPTHDLDAYCDFASGTINIAIMDGKVWRLLNGFKLFREKL
                     DTRRGSNSQLETAVKDLGAVVSFKGYYGDLAIVVAKTSYVAEDGTEKRYLPDGTLVLG
                     NTAAEGIRCYGAIQDAQALSEGVVASSRYPKHWLTVGDPAREFTMTQSAPLMVLPDPD
                     EFVVVQVK"
     gene            6999..7385
                     /locus_tag="OO349_003557"
     CDS             6999..7385
                     /locus_tag="OO349_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HCR5868487.1"
                     /translation="MATKEENLKRLCELAERLGREPDVSGSAADIAQRVAELEEELGD
                     AGEVAEPDTYSPSKDDPTAHKGESVPEQPGIVTGDETGQVTVVALATLHTKCNGSVIF
                     VSPGTSFRVSAEVAASMAAGGLAKRQ"
     gene            7397..7774
                     /locus_tag="OO349_003558"
     CDS             7397..7774
                     /locus_tag="OO349_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5868488.1"
                     /translation="MDGFDNLFDAALAGVNEVILRDMGISAVITSGELEGTHLTGVFD
                     DPESISFVAGGIRLEDSSPCLFVKTADISQLRRQDTLTIGDDSFFVDRITPDDGGCCY
                     IRLRRGSPAPQNNARMRRYDEGT"
     gene            7761..8345
                     /locus_tag="OO349_003559"
     CDS             7761..8345
                     /locus_tag="OO349_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5868489.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQTSAWAVNRVAAKIVSVATRQVAQ
                     NTVAGDNQVKGIPLKTVRERVRLLKASPSGKMYARMRVNRGNLPAIKLGTAQVRLARS
                     RHGSNSRHRGSVLKVGKYLFRDAFIQQLANGRWHVMRRIDGKNRYPIDVVKIPMSGPL
                     TQAFEDARDRIIAAEMPKQLGYALKQQLRLHLSK"
     gene            8342..>8676
                     /locus_tag="OO349_003560"
     CDS             8342..>8676
                     /locus_tag="OO349_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5868490.1"
                     /translation="MNRHTQIRQSVLARLREQCGDSATFFDGLPAFIDAQELPAVAVW
                     LSDAQYTGKMTDEDDWQAVLHIAVFIRAQAPDSELDMWMESTIFPALNDVPALSGLID
                     TLNPLGFNY"
BASE COUNT         2011 a         2055 c         2669 g         1941 t
ORIGIN      
        1 tactcattgc cagatgggaa ctgaccgcag aacaggctgt cgcacagcag ttaaaaaatc
       61 aggtgtcaaa aggaaatctt atcgataccg gattttgtat ttttgccctt agtaagctgg
      121 cgatggcttt atccagtacg cttgactcca ttcctttgtc catgcaacga cagttcccgg
      181 atttaacgcc gcgtcatatt gatcatctga aaatccttat tgctaaggga gcgaatcagt
      241 gcgcgcgtgc gggggataaa ttaccggatt tactcgatga atatatcaga acaacaactg
      301 agtaatatga tggtggcagt cactatcgca ctgcagccac tggtcagggt attgccggtg
      361 acggctgtag aatgggccga tcaaaattat tacctgccta aagaatcgtc atacggtgag
      421 ggggaatgga aaacgctgcc gtttcaggtc gccatcatga actgcatggg caatgacgag
      481 attcgtacag ttaacctgat taaatctgcc cgtgttggtt atacaaaaat ggtgcttggt
      541 gtgatcgggt attttattga gcacaaatcc cgcaacactc ttctttttca gccgacggac
      601 tcagctgctg aagattttat gaagtctcac gtggaagcaa caattcgtga tgttccttgc
      661 ctgaaaaaac tttctccctg gctgggccgg aaacaccggg ataatacgct cacactgaag
      721 cgtttttcct ctggtgtggg gttctggtgt ttgggtggtg ctgctgccaa aaactaccgt
      781 gaaaaatctg tcgatgtggt ctgctatgac gaactctcct cgtttgaacc ggatgtggaa
      841 aaagaaggtt cgccaacact gcttggcgat aaacgtattg aaggttcggt atggccaaaa
      901 tccatacgcg gttcaacgcc aaaaattaaa ggctcctgtc agattgaaaa agctgcgaat
      961 gaatctgcgc atttcatgcg gttttatgtc ccttgccctc attgcgggga ggagcagtat
     1021 ctgaagtttg gcgatgatgc gacgtcgttt ggcctgaaat gggagaaggg taaaccggaa
     1081 acggtgtatt acctgtgtga acataatggc tgcgtgatcc gtcagtctga acttgatcag
     1141 accggggggc gctggatttg tgacaatacc gggatgtgga cgcgtgacgg tctgacgttt
     1201 ttcagtgctt cgggtaatga aattccgccg ccgcgctcca tcactttcca tatatggacg
     1261 gcgtacagtc cgttcaccac ctgggtacag atagtctgtg actggctgga tgcactgaaa
     1321 gatcccaacg gcgtgaaaac ttttgtgaac accacgctgg gcgagacctg ggaagaggcc
     1381 gtgggcgaaa aactcgatca ccaggtactg atggataagg ttgtgcctta cacggcaacg
     1441 gtgcctgtcc gggtggttta tctgacggcg ggcattgact cgcagcgaaa ccgttttgag
     1501 atgtatgtct gggggtgggc tccgggagag gaagcctttc tggtggataa aatcatcatc
     1561 atgggacgtc ctgatgagga agagacgctg ttacgtgtgg atgcggcgat caataaaaaa
     1621 taccgccatg cagacggtac cgaaatgacc atttcccgtg tctgctggga tatcgggggg
     1681 atcgatggcg aaatcgtgta tcagaggtca aaaaaacacg gtgttttccg ggtactgccg
     1741 gtaaaaggtg cctctgtcta tggcaagccg gtgatcacca tgccgaaaac ccgcaatcag
     1801 cggggtgtgt atctgtgtga ggtggggacg gataccgcaa aagaaattct ctatgcccga
     1861 atgaaggccg atcccacccc tgtgaatgag gccacatcgt atgccatccg ctttcctgat
     1921 aatccggaga ttttttcgca gacagaggca caacaactgg tggcggagga actggtggaa
     1981 aagtgggaaa aaggaaagat gcgtctgttg tgggataaca aaaagcggcg taacgaagcg
     2041 ctggactgcc tggtgtatgc ctatgcggca ttacgtgtgt ccgtgcaacg ctggcaactt
     2101 gatctggctg tgctggcggc atcccgcaag gatgcaactg cacaaccgac ccttgaagaa
     2161 ctggcagcga agctgtccgg aggagtgaat ggttacagtc gctgaactgc aggcgctgcg
     2221 tcaggcgcgc cttgatttat taaccggtaa acgggttgtg tcggtacaga aagacggtcg
     2281 caggatcgaa tatacggcag cgtccctgaa tgagcttaac cgtgcgatca acgatgcgga
     2341 gtcggttctg ggtacgaccc gacgccgccg accgctggga gtgaggttat gaaacgaacg
     2401 cctgtcctga ttgatgtgaa cggcgttccg cttcgtgaga gtctcagcta caacggaggc
     2461 ggcgcaggat ttggcgggca aatggcagag tggttaccac cggcgcagag tgccgatgcg
     2521 gccctgttgc ctgcattgcg tctggggaat gcccgggcag atgatctggt gcgcaataac
     2581 ggtatcgcgg ctaatgcggt ggccctgcat aaggatcata tcgtcgggca catgtttctt
     2641 atcagctacc gtctgaactg gcgttggctg gggatgcggg agaccacggc aaaaagtttt
     2701 gtcggggagg tggaagcagc ctggtcggaa tacgcagaag gaatgtttgg cgagatcgac
     2761 gtggaaggga aacgcacgtt tacggaattt atccgtgaag gggtgggcgt gcatgccttt
     2821 aacggcgaaa tctttgtgca gccggtctgg gatacggaaa ccacgcagtt attccgtacg
     2881 cgttttaaag ccgtgagtcc gaaacgggtg gacacgccag gacacggtat ggggaaccac
     2941 tttctgcggg ccggggttga ggtcgatcgg tatgggcggg cgattgcgta ccatatctgt
     3001 gaggatgatt ttccgttctc tggtgccgga cgctgggagc ggatcccgcg tgagcttccc
     3061 accgggcgtc cggccatgct gcatattttt gagccggtgg aggacgggca gacccgtggg
     3121 gccaatcagt tttacagcgt aatggagcgg atgaagatgc tggactccct gcaggccacg
     3181 cagcttcagt cggccattgt gaaggcgatg tatgcagcca caatcgaaag tgaacttgat
     3241 accgaaaagg catttgaata tatcgcgggg gcaccgcaag gtcagcagga taatccgctt
     3301 attaatattc tggagaagtt cacccgctgg tatgacacga ataacgtgac gctgggcggt
     3361 gttaaaattc cgcacctttt ttcctggcga tgcgctgaac ctgcagacag cgcaggattc
     3421 agacaatgga ttttcggcgc ttgaacaggc gctgctgcgg tatattgcag ccggtcttgg
     3481 cgtttcctac gaacagttgt cccgtgatta ctcgaaggtc agttactcaa gtgcccgtgc
     3541 ctccgccaat gagtcgtggc gctattttat ggggcggcga aaatttattg cggcccggct
     3601 ggccacgcag atgttttcct gctggctgga agaggcgctt cttcggggga ttattcgtcc
     3661 gccacgggcg cgttttgatt tttaccaggc gcgttcagcc tggtcacggg cagagtggat
     3721 tggctccgga agaatggcca ttgacgggct caaggaggtc caggaatcgg tgatgcgcat
     3781 tgaggccgga ctgagcacgt atgagaaaga actggcactg atgggcgaag attatcagga
     3841 cattttccgc cagcaggtca gggaatccgc agagcgcgaa aaagccggac tctcacgtcc
     3901 ggtgtggata gcccaggcgt atcagcagca gatagcggag agtcgcaggc cggaagagga
     3961 gacaacatca cgtgagacgt aatctttcac atattatcgc tgcggcattc aatgagccgc
     4021 tgcttctgga gcccgcctat gcgcgggttt tcttttgcgc gctcgggcgt gagatgggcg
     4081 cagcaagcct ttcggtaccg caacagcagg tacagcttga cgctcccggg atgctggctg
     4141 aaacggacga gtacatggcc ggaggcaaac gacctgcccg tgtttaccgg gtggtgaacg
     4201 gtattgctgt actgccggtg accggcacgc tggtacaccg gctgggtggc atgcggccat
     4261 tttccggaat gactggctat gacggcattg tcgcctgtct tcagcaggca atggcggaca
     4321 ctcaggtacg gggcgtactg ctggacattg acagtcctgg cgggcaggcc accggcgcgt
     4381 ttgactgcgc tgacatgatt taccgtctcc ggcagctgaa gcctgtctgg gcactgtgca
     4441 atgacacggc ctgttcagcc gccatgttac tggcatcggc ctgctcccga cggctggtta
     4501 cccagacatc ccgtatcggc tccattgggg tgatgatggg ccatgtcagc tatgccggtc
     4561 atctggcaca ggccggtgtg gatatcacgc tgatttacgc cggatcgcac aaggtggatg
     4621 gcaatcagtt tgaatccctg ccagcaaagg ttcgccagga catgcagcag cgcattgatg
     4681 cggcgcgccg gatgtttgct gaaaaagtgg cgatgtatac cggtttatct gtggatgcgg
     4741 ttatgggaac agaggccgcc gtttttgaag gtcagtccgg tattgaggcc gaactggcgg
     4801 atgaattaat caatgcgtcg gacgccatca gcgtgatggc tgcgacgctg aacacacacg
     4861 atacaggagg cactatgccg caattaactg caacggaggc tgccgcgcag gagaaccagc
     4921 gagtgatggg gatcctgacg tgtcaggaag cgaaaggacg tgaacaactg gccacgatgc
     4981 tggcagggca acagggtatg agcgttgagc aggcccgggc gattctggcc gtggcggcac
     5041 cgcaacaatc tggtgtatct gcgcagagtg aagccgatcg cattatggca tgtgaagagg
     5101 cgaaaggacg tgaacaactg gcggcaacgc tggcggcgat gccggagatg acggtggaaa
     5161 aagcccgtcc gattctggct gcttcaccgc aggcgaatgc cggaccctca cttcgtgatc
     5221 agattatggc cctggatgag gcaaaagggg cagaggcgca ggctgaaaaa ctggcggcct
     5281 gtccggggat gaccgtggag aatgcccggg ctgtactggc ggcagcctca ggcagggcag
     5341 aaccggtttc tgcatccaca accgccatgt ttgaacattt catggcgaac cattcaccgg
     5401 cagcggtaca gggtggcgtg ccacagacgt cagcagacgg tgatgcggac gtgaaaatgc
     5461 tcatggccat gccatgaagt cagtgctgac catcaatatg aggtttttac aatatggtaa
     5521 cgaaaaccat cactgaacag cgtgcggaag tacgtatttt tgccggtaat gatccggccc
     5581 ataccgccac aggcagcagc gggatttcct cggcaacacc ggctctgacg cccctgatgc
     5641 tggatgagga taccgggaaa ctggtggtct gggacggaca gaaggcaggc catgtggttg
     5701 gcatactggt actgccgctt gaaggtacag agacggtgct gacgtattac aagtcgggga
     5761 cctttgcgac ggaggccatc cactggcctg agagagtgga tacacacaaa aaggcgaatg
     5821 cctttgccgg cagtgccctg agtcacgcag cgctgccgta acattttatc aggtcactgc
     5881 ggtagcctga ctgatttctg aatgaaagga actgatttat gggattgttt acgacccgcc
     5941 agttactcgg ttataccgaa caaaaagtta aatttcgtgc gctgtttctg gagctgtttt
     6001 tccgccgtac ggtgaatttc cataccgaag aggtaatgct ggacaaaatt accggaaaaa
     6061 cgccggtggc ggcctatgtc tccccggttg ttgaaggaaa ggtgttgcgt caccgtggcg
     6121 gtgaaactcg tgtactgcgc ccgggctacg ttaagccgaa acacgaattt aattaccagc
     6181 aggcggtgga gcgtcttccc ggtgaagatc cggcccagct gaacgacccg gcttaccgtc
     6241 gcctgcgtat catcaccgat aacctcaaac aggaagagca cgccattgtc caggtggaag
     6301 agatgcaggc ggtgaatgcc gtgctgtatg gcaaatacac catggaagga gagcagtttg
     6361 aaactgttga ggtcgatttt ggccggtcgg tgggaaataa cattacgcag gcaggaggga
     6421 cggagtggag cgctcaggac cgcgatacct ttgatccgac gcatgatctg gatgcgtact
     6481 gtgattttgc ttccggcacc atcaatattg ccattatgga tggtaaggtc tggcgtctgc
     6541 tgaatggctt taagctgttc cgcgaaaaac tggatacccg tcgtggctca aattcacagc
     6601 tggaaacggc agtgaaagac ctgggggcgg tggtgtcctt caaagggtat tatggtgatc
     6661 tggcaatcgt ggtggcaaaa acgtcctatg tggcagagga cggtaccgaa aaacgttatc
     6721 tgccggacgg cacactggta ctgggaaata cggccgcaga gggtattcgt tgctacggtg
     6781 ccattcagga tgcgcaggca ttgtctgaag gcgtggtggc gtcttcccgt tatccgaaac
     6841 actggctgac cgtgggtgat ccggcgcgtg aattcaccat gacgcagtcc gcgccgctga
     6901 tggtgctgcc ggatccggat gagtttgtgg tggtgcaggt gaaataatcc gtgagcggga
     6961 gctaaatgct cccgtttttc attcaatcag gagcgggtat ggctacgaaa gaagaaaacc
     7021 tgaaacgtct ttgtgagctg gctgagaggc tggggcgtga accggatgtc tccggtagtg
     7081 cggcagatat tgcgcagcga gtggcggaac tggaggaaga gcttggagat gcgggggagg
     7141 tcgcagagcc ggatacgtac tcaccatcga aagatgatcc gaccgcgcat aaaggcgagt
     7201 cagttcctga acagccaggc attgttaccg gagacgaaac ggggcaggtt acggtggtgg
     7261 cactggcgac actgcataca aaatgtaatg gttcagtgat atttgtttca ccgggaacgt
     7321 cgtttcgtgt ttctgctgag gtggctgcca gcatggcggc aggtggtctg gcaaaaaggc
     7381 agtaacagga gatgtcgtgg acggtttcga taatctgttt gatgctgcgc ttgccggtgt
     7441 caatgaagtc attctccgtg atatggggat cagcgccgtc attacgtccg gagaacttga
     7501 ggggacacac ctgacagggg tttttgatga tcccgaaagt atttcttttg ttgccggggg
     7561 gatccggctt gaggattcct caccgtgcct gtttgtgaaa acggcagata tatcgcagtt
     7621 acgccgtcag gatacgttaa ctatcgggga tgattctttc tttgtggatc gtattactcc
     7681 ggatgacggg ggatgctgtt atatccggtt aaggcggggc agtcctgccc cacagaataa
     7741 tgccagaatg aggcgatatg atgaagggac ttgagaatgc catccgcaat ctgaacagcc
     7801 tcgatacccg catggtgcca cagaccagcg catgggcggt aaaccgtgtg gcggcaaaga
     7861 ttgtttctgt cgccacacgg caggttgcgc agaataccgt tgccggggat aaccaggtga
     7921 aaggtattcc cctgaaaacg gtcagggagc gtgtcaggct gcttaaagcc agcccctcag
     7981 gaaaaatgta tgcccgcatg cgtgttaacc ggggtaacct gcccgccatt aagctgggta
     8041 ccgcacaggt cagactggcc cgctcccggc atggcagcaa ctcacgtcat cgtggcagcg
     8101 tactgaaagt ggggaaatac cttttccggg atgcgtttat tcagcaactg gcgaatggtc
     8161 gctggcatgt gatgcggcgt attgacggca aaaatcgtta ccccattgat gtggtgaaaa
     8221 tcccgatgtc cggaccgctg acacaggcat ttgaagatgc ccgcgaccgc atcattgctg
     8281 cggaaatgcc gaaacagctg gggtatgccc tgaaacaaca actgaggtta catctgagta
     8341 aatgaaccga cacacacaaa tccgtcagag cgtgctggca cgccttcggg aacagtgtgg
     8401 agacagcgcc acgttttttg acgggcttcc ggcatttatt gatgcgcagg aactgcctgc
     8461 cgtggcggtg tggctgagtg atgctcagta caccggaaaa atgacggatg aagatgactg
     8521 gcaggctgtt ctgcatattg ccgtcttcat ccgggcacag gcaccggatt cagagctgga
     8581 tatgtggatg gagagcacca ttttcccggc cctgaatgat gtaccggcac tttccggact
     8641 catcgacacc ctgaacccac tcggttttaa ctatca
//
LOCUS       DAJTQY010000135         8637 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.135, whole genome shotgun sequence.
ACCESSION   DAJTQY010000135 DAJTQY010000000
VERSION     DAJTQY010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8637
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.135"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(770..1966)
                     /gene="hemY"
                     /locus_tag="OO349_003561"
     CDS             complement(770..1966)
                     /gene="hemY"
                     /locus_tag="OO349_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HCR5868491.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1969..3174)
                     /gene="hemX"
                     /locus_tag="OO349_003562"
     CDS             complement(1969..3174)
                     /gene="hemX"
                     /locus_tag="OO349_003562"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCR5868492.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKIATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTSAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPQGD
                     TPAAAPQGE"
     gene            complement(3196..3936)
                     /gene="hemD"
                     /locus_tag="OO349_003563"
     CDS             complement(3196..3936)
                     /gene="hemD"
                     /locus_tag="OO349_003563"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HCR5868493.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPQLPTYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTVRGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3933..4874)
                     /gene="hemC"
                     /locus_tag="OO349_003564"
     CDS             complement(3933..4874)
                     /gene="hemC"
                     /locus_tag="OO349_003564"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HCR5868494.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLCALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGEAPA"
     gene            5261..7807
                     /gene="cyaA"
                     /locus_tag="OO349_003565"
     CDS             5261..7807
                     /gene="cyaA"
                     /locus_tag="OO349_003565"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HCR5868495.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNLPPANQDHDTPLLQQYFS"
     gene            complement(7847..8167)
                     /gene="cyaY"
                     /locus_tag="OO349_003566"
     CDS             complement(7847..8167)
                     /gene="cyaY"
                     /locus_tag="OO349_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HCR5868496.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDRDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(8215..>8637)
                     /locus_tag="OO349_003567"
     CDS             complement(8215..>8637)
                     /locus_tag="OO349_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HCR5868497.1"
                     /translation="QFHLITGNIAQYKDRLYVTKLSTFGEDQLEIIPLDTPDLVIDRS
                     FNSGKQHAYFIRQLRSKSLQIIPVNGSLTKNDRFAYDDRNVYTWTDTEVRITPSPCPA
                     KTRVREENVREVQNRDIIIPVTDESCRNAAAEVQTLKP"
BASE COUNT         1966 a         2234 c         2220 g         2217 t
ORIGIN      
        1 cagttgacgg ggtatctgct gcgctggcca tgagaggaag agcggcggcg acagcaacaa
       61 caagacgttt gggtgaaaac gaaaattcca tgcaaaatgc tccggtttca tgtcatcaaa
      121 atgatgacgt aattaagcat tgataattga gatccctctc cctgacagga tgattacata
      181 aataatagtg acaaaaataa attatttatt tatccagaaa atgaattgga aaatcaggag
      241 agcgttttca atcctacctc tggcgcagtt gatatgtaag gcaggtttat tatatcgcgt
      301 tgattattga tgctgttttt agttttaacg gcaattaata tatgtgttat taattgaatg
      361 aattttatca ttcataataa gtatgtgtag gatcaagctc aggttaaata ttcactcagg
      421 aagttattac tcaggaagca aagaggatta cagaattatc tcataacaag tgttaaggga
      481 tgttatttcc cgattctctg tggcataata aacgagtaga tgctcattcc atctcttatg
      541 ttcgccttag tgcctcataa actccggaat gacgcagagc cgtttacggt gcttatcgtc
      601 cactgacaga tgtcgcttat gcctcatcag acaccatgga cacaacgttg agtgaagcac
      661 ccacttgttg tcatacagac ctgttttaac gcctgctccg taataagagc aggcgttttt
      721 ttatgcatca gaaaggtccc ggaggtgctt tcctccgggt gagaaggaac tactgtggcg
      781 ggttattctg caacgttaac atcaaaccgt cgcgacgcat cgctgcagct tcttccggct
      841 tgtgcagtct gtccagcgcg tcggcaagcc atgcgtaatc gtaggcgtcc ggacgttgtt
      901 tcagcgccgc gcggaaggcg agcgatgctt cctgccattc accgtgcttc atcagtgact
      961 gacccaatgt gctccacaac agcgggcgat caccgacgtt tttgatttgc tggcgtagca
     1021 ctttttccag ctgttccggg ttattggttt tcagacgcgg gatcggcagc agtaggcgat
     1081 cgtcatactg gcgtttcagg ccgtcgatga taatttgctg ggcagtatca tgatcgtcac
     1141 attcaataag atgttccgcc attgccacct gcaacgctac ctgatgacgc gttttccggc
     1201 tttggttttt ccaccagtta cgcaaacctt cactaccgtt atcggcacgc gcctgatcca
     1261 tcaggccaat ccatgcctgt tgttccagca ttgcacgatg ttcttcatca ccaacatggg
     1321 ctttcgccat tgatgggatg atatccagca gagaactcca tgcccctgtg cggatatacg
     1381 cctgttccgc cagacgtaat acttcaggat ggcgtggcgt aacttccagc agcttatcca
     1441 cgccgtggcg tgcagcatgg ttttcattac gggccagttg cagacgtaca cgggtgattt
     1501 ctaccggaat ggtgtcattg ccggccagct ccgctgcgcg ttccagatgt tggttggcgc
     1561 gtgcttcatc accacgttgt tgcgcggctt cggcagccag tagatagttc accaccggtt
     1621 gttcagcgtg atcggcattt ttcgccatca gcttttcaac ttgctgataa tcaccttccg
     1681 ccagtttcag cagcgcctgt tcggtctgct tacgcgcacg gcgacgctta cgtccgacaa
     1741 accatccacg ggtgtgcgcg ccagtgcgga agatccgccg cagtagccac tcaatggcaa
     1801 acagcactac catcgccaga atcaatatga tcgccaggcc cgtgacgctg gtttcgatat
     1861 tgtagttgtc ggtctggatc agcacataac cctgatgacc ggcaatcatc gggccaacca
     1921 ctatccccgc aatcagcaaa acaaagagta ataacacttt tagcatggtt attctccttg
     1981 cggcgcggct gccggagtat caccttgcgg tgcaggtgca ggtgcaggtg caggtgcagg
     2041 tgcaggttta gcttccgttg tccccgctgc cggttgtgcc agcaggttac gcacgcgagt
     2101 ctgcatcagt ttttccagca tcgcctggct ttgcagggtt tccggaagat ccatcgagat
     2161 attttgctgg cttaactggt ccacctcgtc gaggaacgct ttggtggtgg catcatcagt
     2221 atcgtagtaa gcacgtaccc aggtggagac gttctccagc gcctggcgat aagtctcttc
     2281 ctgatgacgc ggtacagctt gtgctgcgac cagcaggcga gagcgaatat tttcgcgcag
     2341 atagatatcc tgatttggcg ctaacagcgg tacggcggtg tcatcacgac ggcgaatcgt
     2401 aatgaagttg tccataaagt tctgccagct tttttgcaga ttgatacgcc attcgctgat
     2461 ggaactggaa agctcttcac cgtctgaatc catcggcgaa ccatcgctgt cattatcggc
     2521 cagacgcagg ttatctacct gatttgaaag ctgattaagc ttaaggatga tgccgtcata
     2581 atccacctgc gatactgcag aaaggctggc gatatcatcg gtaattgccc gacgaacggt
     2641 aatcagactc gggtcattca tatccgccag gctggcgtct gcacttttca gcaacgctgc
     2701 agaggtcgtg acgtcctgat cgctccacag cttccgtccg gcgagtttca ccagaaaatc
     2761 ggcctgagcc agcagccagg ttttagcatc gctgccggaa atggtggcga tcttttgttg
     2821 gacttcatcc aactgttttg ccagcgtttc ttgctgacga ttcgcctgct taagctgcgc
     2881 agcttgttgc ttaataatgc cttccagctc ggctttttgg ctctcctggg ctttttgcaa
     2941 tgccgtcagt tggttagcca gggcatcgct ggtggcggtc tgattgacgg cctgttgttt
     3001 accccagcca tacaaaccga cacccgccgc cagagcaata gcgatagcca ccgcgctgag
     3061 aatcaatgca gtattgttct tactcttttt ttctgttgcg acaggttgtg acgtggtgtc
     3121 cacggcctcc ctggtctctt caaccacggc ggaggttttt tcttgttccg tcattatggc
     3181 ttcctgttat gagagttatt gtaatgcccg taaaagcgca tcgttgtcag cgttatcggc
     3241 gaccttaatg tcttgccagc ccagttcccg ggcgagtttc gccaaacgct cactgacgac
     3301 caatagtcga cagtgtagta accagtgctc acgataccat tgtgggatca gcgaccagag
     3361 ttgctgcaac atttcaccgc tggtaacaac gaccatcgtc acctcgcggg attgccagcg
     3421 catcgcttct tctgcaccat cgtaatggat tgcgcatcgt tgataacatt cacaaaaagt
     3481 gacctcagca ccgcgcaccg tcagggtatc cccaattagc tcacgaccgc cattgccacg
     3541 taatatcagc gcacgtttgc ccgcaatatt ttgtaattca ggtaattgta gcaagacttc
     3601 gctgatttcc cgatcctgtg ggtagagaat cttctgtccg cttacggtat gtagcgccag
     3661 tgcggtggtg cgtccaatgg cgaaataagt aggtagttgg ggccatttac gatcttgctg
     3721 atgcagctgt gattgggcaa aagcaaccgc gtgttgcgag agggcaaaca acagatcgct
     3781 ctcccccagc gctgccagtt gatcagcaag ttgcggtaat tgtcgacccg gagaaaactc
     3841 aatcagcgga aaatgccagg ccacctgccc cagtgtgcgc agacggctca ctaactcttc
     3901 tccagcggga gacgggcggg tgaccaggat actcatgccg gggcctctcc gttatagact
     3961 tcagcgagga tctcgcgcgc gccgttattc agtagctctt ctgccagcga aatccccatt
     4021 tgttcggcat cttgcggcgc accgcggcgt tcaccgcgaa taatctgcga accgtccggc
     4081 gcgccgacca gcgcacatag ccagatttcg ccatcaataa gctcggcgta gctaccaatt
     4141 ggcacctgac atccgccttc gagacgggta ttcatggcgc gttctgcggt aacgcgcagt
     4201 gcagtttcgt ggtgattcag cgcggcaagc agctcgcgag tgcgtgtatc atcaaggcgg
     4261 cattcaatac cgactgcacc ttgtcctacc gccggaagag aaatctcggg tggtaacgcg
     4321 gcgcgaatgc gggactccag acctaaacgt tttagtccgg ctacggcaag aatgatggca
     4381 tcgtattcgc cgttatctag tttgctcagg cgagtgccga cgttgccgcg cagggagcgg
     4441 ataatcagat ccgggcggcg ttcagccagt tggcactggc gacgtaaact ggacgtcccg
     4501 acgatactgc ctgctggtaa cgcatccaga ctgtcatagt tattggacac aaaggcatcg
     4561 cgaggatctt cacgctcgca aatagtgacc agccccagac cttgcgggaa ttcaaccggc
     4621 acatctttca ttgaatgtac ggcgatatca gcgcgattct ccaggagcgc gacttccagc
     4681 tcttttacaa ataagccttt tccgcctact ttcgccagcg gcgtatcaag aatcacatcg
     4741 ccgcgcgtca ccatcggtac cagttcaacg accaggcccg gatggctcgc catcaacttg
     4801 tctttgacat agtgtgcctg ccagagtgca agtgggcttt ggcgtgtggc aattcttaaa
     4861 acattgtcta acatgcttgt taccgtcatt atcatccgtg gtccatccta acatccttgc
     4921 cagagtgatg tcagtgttgt ggtgaaacgt agacgcctgc gcaaaccgta aaatgaggtc
     4981 tggcagtgga tcctgacagg cgtttcacgc cgtcgtaata aggaatttac agagaataaa
     5041 cggtgctaca cttgtatgta gcgcatcttt ctttacggtc aatcagcaag gtgttaaatt
     5101 gatcacgttt tagaccattt tttcgtcgtg aaactaaaaa aaccaggcgc gaaaagtggt
     5161 aacggttacc tttgacatac gaaatatccc gaatgccgcg tgttaccgtt gatgttggcg
     5221 gaatcacagt catgacgggt agcaaatcag gcgatacgtc ttgtacctct atattgagac
     5281 tctgaaacag agactggatg ccataaatca attgcgtgtg gatcgcgcgc ttgctgctat
     5341 ggggcctgca ttccaacagg tctacagtct actgccgaca ttgttgcact atcaccatcc
     5401 gctaatgccg ggttaccttg atggtaacgt tcccaaaggc atttgccttt acacgcctga
     5461 tgaaactcaa cgccactacc tgaacgagct tgaactgtat cgtggaatgt cagtacagga
     5521 tccaccgaaa ggtgagcttc caattactgg tgtatacacc atgggcagca cctcgtccgt
     5581 agggcaaagt tgttcctctg acctggatat ctgggtctgt catcaatcct ggctcgatag
     5641 cgaagagcgc cagttactac aacgtaaatg cagcctgctg gaaagctggg ccgcctcgct
     5701 gggtgtggaa gtcagcttct tcctgattga tgaaaaccgc ttccgtcata atgaaagcgg
     5761 cagcctgggg ggcgaagatt gtggctccac ccagcatata ctgctgcttg acgaatttta
     5821 tcgtactgcc gtgcgtctcg ccggtaagcg tattctgtgg aatatggtgc cgtgcgacga
     5881 agaagagcat tacgacgact atgtgatgac gctttacgcg cagggcgtgc tgacgccaaa
     5941 tgaatggctg gatctcggtg gcttaagctc gctttctgct gaagagtact ttggtgccag
     6001 cctttggcag ctctacaaga gtatcgattc cccatacaaa gcggtgctga aaacactgct
     6061 gctggaagcc tattcctggg aatacccgaa cccacgtctg ctggcgaaag atatcaaaca
     6121 gcgtttgcac gacggcgaga ttgtatcgtt tggcctcgat ccatactgca tgatgctgga
     6181 gcgtgttact gaatacctga cggcgattga agatttcacc cgtctggatt tagtacgtcg
     6241 ctgcttctat ttaaaagtgt gcgaaaaact cagccgtgaa cgcgcctgtg taggctggcg
     6301 tcgcgcagtg ttgagccagt tagtgagcga gtggggttgg gacgaagctc gtctggcaat
     6361 gctcgataac cgtgctaact ggaagattga tcaggtgcgt gaggcgcaca acgagctgct
     6421 cgacgcgatg atgcagagct accgtaatct gatccgcttt gcgcgtcgca ataaccttag
     6481 cgtctccgcc agtccgcagg atatcggcgt actgacgcgt aaactgtatg ccgcgtttga
     6541 agcattgcca ggtaaagtga cgctggtaaa cccgcagatt tcacccgatc tctcggaacc
     6601 gaatctgacc tttatttatg tgccgccggg ccgggctaac cgttcaggtt ggtatctgta
     6661 taaccgcgcg ccaaatattg agtcgatcat cagccatcag ccgctggaat ataaccgtta
     6721 cctgaataaa ctggtggcgt gggcatggtt taacggcctg ctgacctcgc gcacccgttt
     6781 gtatattaaa ggtaacggca ttgtcgattt gcctaagttg caggagatgg tcgccgacgt
     6841 gtcgcaccat ttcccgctgc gcttacctgc accgacaccg aaggcgctct acagcccgtg
     6901 tgagattcgc catctggcga ttatcgttaa cctggaatat gacccgacag ctgcgttccg
     6961 caatcaggtg gtgcattttg atttccgtaa gctggatgtc ttcagctttg gcgagaatca
     7021 aaattgcctg gtaggtagcg ttgacctgct gtaccgcaac tcgtggaacg aagtgcgtac
     7081 gctgcacttc aacggcgagc aatcgatgat cgaagccctg aaaactattc tcggcaaaat
     7141 gcatcaggac gccgcaccgc cagatagcgt ggaagtcttc tgttatagcc agcatctgcg
     7201 cggcttaatc cgtactcgcg tgcagcaact ggtttctgag tgtattgaac tgcgcctttc
     7261 cagcacccgc caggaaaccg ggcgtttcaa ggcgctgcgc gtttctggtc aaacctgggg
     7321 cttgttcttc gaacgcctga atgtatcggt acagaagctg gagaacgcca tcgagtttta
     7381 tggcgctatt tcgcataaca aactgcacgg cttgtcagtg caggttgaaa ccaatcacgt
     7441 caaattaccg gcggtggtgg acggctttgc cagcgaaggg atcatccagt tctttttcga
     7501 agaaacgcaa gacgagaatg gctttaatat ctacattctc gacgaaagca accgggttga
     7561 ggtgtatcac cactgcgaag gcagcaaaga ggagctggta cgtgacgtca gtcgcttcta
     7621 ctcgtcatcg cacgaccgct tcacctacgg ctcaagcttc atcaacttca acctgccgca
     7681 gttctatcag attgtgaagg ttgatggtcg cgaacaggtg attccgttcc gaacaaaatc
     7741 gattggcaac ctgccgcccg ccaatcagga tcacgatacg ccgctattac agcagtattt
     7801 ttcgtgatga gcgtgccgga aagcgaggct tatccggcat gcaatcttag cggaaactga
     7861 ctgtttcacc cgcctgttgc gtcgccgcct gttccagcaa atcccagaag gtttcgccgc
     7921 tgcgatcgca aatccattca tcgcctttca ggtcaaaatg gtagccgccc tgtttggttg
     7981 ccagccacac ctggtgcagc ggctcctggc ggttaataat gattttgctg ccgttttcaa
     8041 aggtaatggt cagtacgccg ccgttgattt cgcagtcgat atcgctgtcg ccatccctgt
     8101 cgtccagacg ctcttcaatg gtcagccaca gttgatcagc caggcgatga aattcactgt
     8161 cgttcattgt tgtatcctgt ttttaagtga tggcggcagt atagcggcat ggggtcaggg
     8221 cttcaaagtt tgcacctctg ctgctgcgtt ccggcatgat tcatccgtca ccggaataat
     8281 gatgtctctg ttttgaactt cacgtacgtt ctcctctctg acacgagttt tcgccgggca
     8341 gggggagggc gtaatcctta cctctgtatc ggtccatgta taaacattgc gatcgtcata
     8401 agcgaagcga tcgtttttag ttagcgaacc gttaactgga ataatttgca agctctttga
     8461 ccgtaattgg cggataaagt aagcatgctg tttgccgcta ttaaatgagc gatcaatgac
     8521 caggtctggc gtatccagcg ggattatctc aagctggtct tcaccaaatg tcgataattt
     8581 tgttacatac agccgatctt tatattgcgc aatattgcca gtaatcagat ggaattg
//
LOCUS       DAJTQY010000136         8395 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.136, whole genome shotgun sequence.
ACCESSION   DAJTQY010000136 DAJTQY010000000
VERSION     DAJTQY010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8395
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.136"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            3..816
                     /gene="ttdA"
                     /locus_tag="OO349_003568"
                     /pseudo
     CDS             3..816
                     /gene="ttdA"
                     /locus_tag="OO349_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000445032.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
     gene            813..1418
                     /gene="ttdB"
                     /locus_tag="OO349_003569"
     CDS             813..1418
                     /gene="ttdB"
                     /locus_tag="OO349_003569"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HCR5868498.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            1466..2929
                     /gene="ttdT"
                     /locus_tag="OO349_003570"
     CDS             1466..2929
                     /gene="ttdT"
                     /locus_tag="OO349_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HCR5868499.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPVPAGLENHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVTLIVVFYLLRY
                     FFASATAYTCALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(2972..3985)
                     /gene="tsaD"
                     /locus_tag="OO349_003571"
     CDS             complement(2972..3985)
                     /gene="tsaD"
                     /locus_tag="OO349_003571"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HCR5868500.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            4223..4438
                     /gene="rpsU"
                     /locus_tag="OO349_003572"
     CDS             4223..4438
                     /gene="rpsU"
                     /locus_tag="OO349_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HCR5868501.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            4549..6294
                     /gene="dnaG"
                     /locus_tag="OO349_003573"
     CDS             4549..6294
                     /gene="dnaG"
                     /locus_tag="OO349_003573"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCR5868502.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            6489..8330
                     /gene="rpoD"
                     /locus_tag="OO349_003574"
     CDS             6489..8330
                     /gene="rpoD"
                     /locus_tag="OO349_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HCR5868503.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT         2027 a         2253 c         2252 g         1863 t
ORIGIN      
        1 cagtgaataa gttgacagag attgtcgcta actttaccgc catgatttct acccgaatgc
       61 ctgatgacgt ggtggataaa ctaaaacagc taaaggatgc cgaaacgtcg tcgatgggga
      121 aaattatcta ccatacgatg ttcgacaaca tgcaaaaagc gattgacctg aatcgtcctg
      181 cctgtcagga caccggggag attatgttct tcgttaaagt cggttcccgc ttcccactgc
      241 ttggcgagct gcaaagcata ctcaaacaag ccgtggaaga ggcaaccgtc aaagcgccac
      301 tacgtcacaa tgcggtagaa atttttgacg aagtaaacac cggcaaaaat accggtagcg
      361 gcgtaccgtg ggtcacctgg gacatcatcc ccgacaatga cgatgcggaa atcgaagttt
      421 acatggcagg cggcggctgc acgctacctg gccgctcgaa agtgttaatg ccgtcagaag
      481 gctacgaagg cgtggtgaaa ttcgtcttcg aaaatatctc caccctcgcc gtaaacgcct
      541 gtccaccggt actggtgggc gtgggcatcg ccacctcggt ggaaaccgcc gccgtactct
      601 cgcgtaaagc cattttgcgc ccgattggca gccgccatcc caatccaaaa gcggcagaac
      661 tggagctacg cctggaagaa ggactcaacc gtctggggat tggtccacaa gggctgaccg
      721 gcaacagttc agtgatgggc gcatcgtcgc ggcacgctgc tggttcatgc cgatctcacc
      781 tttgaaaatc tgtctcacac ccggagcgcg ttatgaaaaa gatcctgaca accccgatca
      841 aagctgaaga tctgcaagat attcgcgtcg gcgatgtgat ctacctgacc ggtacgctgg
      901 tgacctgccg cgacgtttgt caccgccgtt tgatcgaact gaaacgtccg atcccttacg
      961 atctcaacgg caaagcgatt ttccacgctg gccccatcgt gcgcaaaaac ggtgacaaat
     1021 gggagatggt ctccgtcggc ccgacaacca gtatgcgtat ggaaagtttt gaacgtgaat
     1081 ttattgagca gaccggcgta aaactggtgg ttggcaaagg tggtatgggg ccgctgaccg
     1141 aagaaggctg ccagaaattc aaggcgttac atgtgatttt cccagcaggc tgcgcggtgc
     1201 tggcggcaac ccaggtagaa gagattgaag aagtgcactg gacagaactc gggatgccgg
     1261 agtcactgtg ggtctgccgg gtcaaagagt tcggcccgct gattgtctct attgataccc
     1321 acggcaacaa cctgatagcc gaaaacaaaa agctgttcgc cgaacgccgc gatcccatcg
     1381 tggaagagat ctgcgagcac gtccattaca tcaaataacc ctcccggaga ggctcacccc
     1441 tctccttttt cgcaggcata acacgatgaa accttccact gaatggtggc gatatcttgc
     1501 gccgctggcg gtcatcgcca ttattgctct acttccggtt cccgcagggc tggagaacca
     1561 tacctggctc tactttgccg tttttactgg cgtgatcgtt ggactgatcc tcgaacccgt
     1621 gccgggtgcc gtggtggcga tggtgggtat ctccatcatc gccatactct ctccctggct
     1681 gctgttcagc cccgaacagc tcgcccagcc aggctttaag ttcaccgcca aatccctctc
     1741 ctgggcagtt tctggttttt ctaattcggt tatctggctc attttcgccg cctttatgtt
     1801 tggcacaggc tatgaaaaaa ccgggcttgg acgccgcatt gcgctgattc tggtgaaaaa
     1861 gatggggcat cgcacgctgt ttctcggcta tgcggtgatg ttctccgagc tgatcctggc
     1921 acctgtaaca ccgtccaact cggcgcgtgg tgcgggtatt atctatccca tcatccgtaa
     1981 cctgccaccg ctctatcaat cacaaccaaa cgacagcagt tcgcgcagca ttggctcgta
     2041 catcatgtgg atggggattg ttgccgactg cgtgaccagc gccattttcc tgacggcgat
     2101 ggcacctaac ttgctgttaa ttggactgat gaaaagcgca tctcacgcca cgctgagttg
     2161 gggcgactgg ttcctcggga tgttgccgct cagcatttta ctggttctgc tggttccctg
     2221 gctggcttac gtgctgtacc cgccggtact gaagtctggt gatcaggtgc cgcgctgggc
     2281 agagacggaa ctgcaggcaa tgggcccgct ctgttcgcgt gaaaaacgga tgctggggct
     2341 gatggtaggc gcgctggtgc tgtggatttt cggcggtgat tatatcgatg ccgcgatggt
     2401 cggttacagc gtggtggcgc tgatgctgct tctgcgcatt atcagttggg acgacattgt
     2461 cagtaataaa gcggcatgga acgttttctt ctggctggcc tcgcttatca ccctcgcgac
     2521 cggactcaac aataccggtt ttattagctg gtttggcaaa ctgttagcag gcagcttaag
     2581 tggttattcg ccgacgatgg tgatggtgac gttgattgtg gtgttttatc tactgcgcta
     2641 ctttttcgcc agcgccacgg cgtatacctg cgccctcgca ccgatgatga tcgccgccgc
     2701 gctggcgatg ccggaaatcc cgctgccggt gttctgcctg atggttggcg cggcaattgg
     2761 tctggggagc attcttacac catacgccac cggacccagt ccgatttact acggtagtgg
     2821 ttatctgcca acggcggatt actggcgact gggggcgatt tttgggctga tattcctcgt
     2881 gttgctggtg attaccggct tactgtggat gcccgtggtg ttgctttaag tgaggacggt
     2941 gccggaaatt gctctccggc acccaatagt tttacgcagc cggtaactcc gccagcggcc
     3001 agcgcggacg cacgctaaca ccgagatccg ccgtcgcgcc tgctttaaac cgcaccattc
     3061 cggcataggc gatcatcgcg ccgttatcag tacaaaattc cggacgcgcg tagaacactt
     3121 cgccgcggcg ttttttcatc atttcagcca gcttcgcccg taacgtgcgg ttagcactca
     3181 cgccgcccgc catgaccagt cgcttaaagc ccgtctgatc caacgctcgc ttacacttaa
     3241 tcatcaacgt atcgaccacc gcatcttcaa aggcgcgggc gatatcagca cgcgtctggt
     3301 cgtcggtgcc gttgtcacga atcgtgttcg ccgcaaaggt tttcagacca gaaaagctga
     3361 aatccagccc cggacggtcg gtcatcggac gcgggaaaac aaagcgcccg gcagtaccct
     3421 gagccgccat tttcgacagt aacggtccgc caggataatc cagccccagc agcttcgcgg
     3481 ttttatcaaa cgcttcaccg gcggcatcat cgatagactc gccgagcagc tcgtactgac
     3541 caatgccagt cacgctgatt aactgcgtat gaccgccgga caccagcagc gcgacaaacg
     3601 gaaattccgg cgggttatct tccagcatcg gcgctaacag atgcccttcc atatggtgta
     3661 ccgggattgc cggaacgttc caggcaaacg ccagagaacg ccccacggtt gcgccaacca
     3721 gcagtgcgcc gactaatcca gggcctgcgg tataggccac agcatcaatg tcttttgccg
     3781 ttaaaccaga ctcctttagc gccgcctgga tcaacggtac ggttttacgc acatgatcgc
     3841 gggaggccag ttcaggcacg acgccgccgt agtcagcgtg caatttcacc tgactataca
     3901 attggttggc taacaaacct ttttcatcgt cgtaaatggc gatgccggtt tcatcgcagg
     3961 aagtttcaat acccagtaca cgcatgactt gttttacctc gctttattac cgcgcagtgt
     4021 aggaccaatg cgggttgatg taaaactttg ttcgcccctg gagaaagcct cgtgtatact
     4081 cctcaccctt ataaaagtcc ctttcaaaaa aggccgcggt gctttacaaa gcagcagcaa
     4141 ttgcagtaaa attccgcacc attttgaaat aagctggcgt tgatgccagc ggcaaaccga
     4201 attaatcaaa ggtgagaggc acatgccggt aattaaagta cgtgaaaacg agccgttcga
     4261 cgtagctctg cgtcgcttca agcgttcctg cgaaaaagca ggtgttctgg cggaagttcg
     4321 tcgtcgtgag ttctatgaaa aaccgactac cgaacgtaag cgcgctaaag cttctgcagt
     4381 gaaacgtcac gcgaagaaac tggctcgcga aaacgcacgc cgcactcgtc tgtactaatt
     4441 ccccgagagc gttgctctcc gatcagaccg agttgtagtt gtaaggccgt gcttccgaaa
     4501 ggaatgcgcg gcttattttc gtttatgaat tgctaaaaat cggggcctat ggctggacga
     4561 atcccacgcg tattcattaa tgatctgctg gcacgcactg acatcgtcga tctgatcgat
     4621 gcccgtgtga agctgaaaaa gcagggcaag aatttccacg cgtgttgtcc attccacaac
     4681 gagaaaaccc cgtcattcac cgttaacggt gagaaacagt tttaccactg ctttggatgt
     4741 ggcgcgcacg gcaacgcgat cgacttcctg atgaactacg acaagctcga gttcgtcgaa
     4801 acggtcgaag agctggcagc aatgcacaat cttgaagtgc catttgaagc aggcagcggc
     4861 cccagccaga tcgagcgcca tcagaggcaa acgctttatc agttgatgga cggtctgaat
     4921 acgttttacc aacaatcttt acaacaacct gttgccacgt ctgcgcgcca gtatctggaa
     4981 aaacgcggat taagccacga ggttatcgct cgctttgcga ttggttttgc gccccccggc
     5041 tgggacaacg tcctgaagcg gtttggcggc aatccagaaa atcgccagtc attgattgat
     5101 gcggggatgt tggtcactaa cgatcaggga cgcagttacg atcgtttccg cgaacgggtg
     5161 atgttcccca ttcgcgataa acgcggtcgg gtgattggtt ttggcgggcg cgtgctgggc
     5221 aacgataccc ccaaatacct gaactcgccg gaaacagaca ttttccataa aggccgccag
     5281 ctttacggtc tttatgaagc gcagcaggat aacgctgaac ccaatcgtct gcttgtggtc
     5341 gaaggctata tggacgtggt ggcgctggcg caatacggca ttaattacgc cgttgcgtcg
     5401 ttaggtacgt caaccaccgc cgatcacata caactgttgt tccgcgcgac caacaatgtc
     5461 atttgctgtt atgacggcga ccgtgcaggc cgcgatgccg catggcgagc gctggaaacg
     5521 gcgctgcctt acatgacaga cggccgtcag ctacgcttta tgtttttgcc ggatggcgaa
     5581 gaccctgaca cgctggtacg aaaagaaggt aaagaagcgt ttgaagcgcg gatggagcag
     5641 gcgatgccac tctccgcatt tctgtttaac agtctgatgc cgcaagttga tctgagtacc
     5701 cctgacgggc gcgcacgttt gagtacgctg gcactgccat tgatatcgca agtgccgggc
     5761 gaaacgctgc gaatatatct tcgtcaggaa ttaggcaaca aattaggcat acttgatgac
     5821 agccagcttg aacgattaat gccaaaagcg gcagagagcg gcgtttctcg ccctgttccg
     5881 cagctaaaac gcacgaccat gcgtatactt atagggttgc tggtgcaaaa tccagaatta
     5941 gcgacgttgg tcccgccgct tgagaatctg gatgaaaata agctccctgg acttggctta
     6001 ttcagagaac tggtcaacac ttgtctctcc cagccaggtc tgaccaccgg gcaactttta
     6061 gagcactatc gtggtacaaa taatgctgcc acccttgaaa aactgtcgat gtgggacgat
     6121 atagcagata agaatattgc tgagcaaacc ttcaccgact cactcaacca tatgtttgat
     6181 tcgctgcttg aactgcgcca ggaagagtta atcgctcgtg agcgcacgca tggtttaagc
     6241 aacgaagaac gcctggagct ctggacatta aaccaggagc tggcgaaaaa gtgatttaac
     6301 ggcttaagtg ccgaagagcg atcgggaagc ccccgacagc cgcactgaga ggcagcggca
     6361 aatatataag tacgccctcg taattatcgt tggcggtaaa caaccgttgg atttcagcgt
     6421 taacggctga aggacatcgg gtcaatcgcc caacaccaac ctcatgaaat aagtgtggat
     6481 accgtcttat ggagcaaaac ccgcagtcac agctgaaact tcttgtcacc cgtggtaagg
     6541 agcaaggcta tctgacctat gccgaggtca atgaccatct gccggaagat atcgtcgatt
     6601 cagatcagat cgaagacatc atccaaatga tcaacgacat gggcattcag gtgatggaag
     6661 aagcaccgga tgccgatgat ctgatgctgg ctgaaaacac cgcggacgaa gatgctgccg
     6721 aagccgccgc gcaggtgctt tccagcgtgg aatctgaaat cgggcgcacg actgacccgg
     6781 tacgcatgta catgcgtgaa atgggcaccg ttgaactgtt gacccgcgaa ggcgaaattg
     6841 acatcgctaa gcgtattgaa gacgggatca accaggttca atgctccgtt gctgaatatc
     6901 cggaagcgat cacctatctg ctggaacagt acgatcgtgt tgaagcagaa gaagcgcgtc
     6961 tgtccgatct gatcactggc tttgttgacc cgaacgcaga agaagatctg gcacctaccg
     7021 ccactcacgt cggttctgag ctttcccagg aagatctgga cgatgacgaa gatgaagacg
     7081 aagaagatgg cgatgacgac agcgccgatg atgacaacag catcgacccg gaactggctc
     7141 gcgaaaaatt tgcggaacta cgcgctcagt acgttgtaac gcgtgacacc atcaaagcga
     7201 aaggtcgcag tcacgctgcc gctcaggaag agatcctgaa actgtctgaa gtattcaaac
     7261 agttccgcct ggtgccgaag cagtttgact acctggtcaa cagcatgcgc gtcatgatgg
     7321 accgcgttcg tacgcaagaa cgtctgatca tgaagctctg cgttgagcag tgcaaaatgc
     7381 cgaagaaaaa cttcattacc ctgtttaccg gcaacgaaac cagcgatacc tggttcaacg
     7441 cggcaattgc gatgaacaag ccgtggtcgg aaaaactgca cgatgtctct gaagaagtgc
     7501 atcgcgccct gcaaaaactg cagcagattg aagaagaaac cggcctgacc atcgagcagg
     7561 ttaaagatat caaccgtcgt atgtccatcg gtgaagcgaa agcccgccgt gcgaagaaag
     7621 agatggttga agcgaactta cgtctggtta tttctatcgc caagaaatac accaaccgtg
     7681 gcttgcagtt ccttgacctg attcaggaag gtaacatcgg cctgatgaaa gcggttgata
     7741 aattcgaata ccgccgtggt tacaagttct ccacctacgc aacctggtgg atccgtcagg
     7801 cgatcacccg ttctatcgcg gatcaggcgc gcaccatccg tattccggtg catatgattg
     7861 agactatcaa caaactcaac cgtatttctc gccagatgct gcaagagatg ggccgtgaac
     7921 cgacgccgga agaactggct gaacgtatgc tgatgccgga agacaagatc cgcaaagtgc
     7981 tgaagatcgc caaagagcca atctccatgg aaacgccgat cggtgatgat gaagattcgc
     8041 atctggggga tttcatcgag gataccaccc tcgagctgcc gctggattct gcgaccaccg
     8101 aaagcctgcg tgcggcaacg cacgacgtgc tggctggcct gaccgcgcgt gaagcaaaag
     8161 ttctgcgtat gcgtttcggt atcgatatga acaccgacca cacgctggaa gaagtgggta
     8221 aacagttcga cgttacccgc gaacgtatcc gtcagatcga agcgaaggcg ctgcgcaaac
     8281 tgcgtcaccc gagccgttct gaagtgttgc gtagcttcct ggacgattaa tcggtaggcc
     8341 ggatcaggcg ttacgccgca cccggcacta gtctctctgc acaaacgcca ccttt
//
LOCUS       DAJTQY010000137         8176 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.137, whole genome shotgun sequence.
ACCESSION   DAJTQY010000137 DAJTQY010000000
VERSION     DAJTQY010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8176
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.137"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            <1..1589
                     /gene="maeA"
                     /locus_tag="OO349_003575"
     CDS             <1..1589
                     /gene="maeA"
                     /locus_tag="OO349_003575"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCR5868504.1"
                     /translation="RNFNLLGLLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQ
                     DTNETLFYRLVNNHLDEMMPVIYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDIL
                     QNVPNHNIKVIVVTDGERILGLGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDV
                     GTNNQQRLNDPLYMGWRNPRITDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMP
                     LLNRYRNEICSFNDDIQGTAAVTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEM
                     IIAQTQREGLSEEAARQKVFMVDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDV
                     LSLLDVVRNVKPDILIGVSGQTGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQD
                     IIAWTEGNALVATGSPFNPVVWKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEML
                     MSASETLAQYSPLVLNGEGLVLPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQ
                     QAIDDNFWQAEYRDYRRTSI"
     gene            1723..2733
                     /gene="adhP"
                     /locus_tag="OO349_003576"
     CDS             1723..2733
                     /gene="adhP"
                     /locus_tag="OO349_003576"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HCR5868505.1"
                     /translation="MKTAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAPSSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMDADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RH"
     gene            2879..3163
                     /locus_tag="OO349_003577"
     CDS             2879..3163
                     /locus_tag="OO349_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCR5868506.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVVVSRLRRLVTQ"
     gene            complement(3391..4044)
                     /gene="fdnI"
                     /locus_tag="OO349_003578"
     CDS             complement(3391..4044)
                     /gene="fdnI"
                     /locus_tag="OO349_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HCR5868507.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(4037..4921)
                     /gene="fdnH"
                     /locus_tag="OO349_003579"
     CDS             complement(4037..4921)
                     /gene="fdnH"
                     /locus_tag="OO349_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HCR5868508.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(4934..7981)
                     /gene="fdnG"
                     /locus_tag="OO349_003580"
     CDS             complement(4934..7981)
                     /gene="fdnG"
                     /locus_tag="OO349_003580"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(7394..7396),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCR5868509.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPKYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGILRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETQAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
BASE COUNT         1946 a         2205 c         2082 g         1943 t
ORIGIN      
        1 gccgtaactt caacctgctg gggttactgc cggaagtggt cgaaaccatc gaagaacaag
       61 cggaacgagc atggatccag tatcagggat tcaaaaccga aatcgacaaa cacatctacc
      121 tgcgtaacat ccaggacacc aacgaaaccc tcttctaccg tctggtaaac aatcatcttg
      181 atgagatgat gcctgttatt tataccccaa ccgtcggcgc agcctgtgag cgtttttctg
      241 agatctaccg ccgttcacgc ggcgtgttta tctcttacca gaaccgccac aatatggacg
      301 atattctgca aaacgtgccg aaccataata ttaaagtgat tgtggtgact gacggtgaac
      361 gtattctggg gcttggtgac cagggcatcg gcgggatggg cattccgatc ggtaaactgt
      421 cgctctatac cgcctgtggc ggcatcagcc cggcgtatac ccttccggtg gtgctggatg
      481 tcggaacgaa caaccaacag cggcttaacg atccgctgta tatgggctgg cgtaatccgc
      541 gtatcactga cgacgaatac tatgaattcg ttgatgaatt tatccaggct gtgaaacaac
      601 gctggccgga cgtgctgttg cagtttgaag actttgctca aaaaaatgcg atgccgttac
      661 ttaaccgcta tcgcaatgaa atttgttctt ttaacgatga cattcagggc actgcggcgg
      721 taacagtcgg cacactgatc gcagccagcc gcgcggcagg tggtcagtta agcgagaaaa
      781 aaatcgtctt ccttggcgca ggttcagcgg gatgcggcat tgccgaaatg atcatcgccc
      841 agacccagcg cgaaggatta agcgaggaag cagcgcggca gaaagtcttt atggtcgatc
      901 gctttggcct gctgacggac aagatgccga acctgctgcc tttccagacc aaactggtgc
      961 agaagcgcga aaacctcagt gactgggata ccgacagcga tgtgttgtca ctgctggatg
     1021 tggtgcgcaa tgtaaaacca gatattctga ttggcgtctc aggacagacc gggctgttta
     1081 cggaagagat catccgtgag atgcataaac actgtccgcg tccgatcgtg atgccgctgt
     1141 ctaacccgac gtctcgcgtg gaagccacac cgcaggacat tatcgcctgg accgaaggta
     1201 acgcgctggt cgccactggc agcccgttta atcctgtggt atggaaagat aaaatctacc
     1261 ctatcgccca gtgtaacaac gcctttattt tcccgggcat cggcctgggt gttattgctt
     1321 ccggcgcgtc acgtatcacc gatgagatgc tgatgtcggc aagtgaaacg cttgctcagt
     1381 attcgccgct ggtcctgaac ggcgaaggtc tggtactacc ggaactgaaa gatattcaga
     1441 aagtctcccg cgcaattgcg tttgcggttg gcaaaatggc gcagcagcaa ggcgtggcgg
     1501 tcaaaacgtc tgctgaagct ttgcaacaag ccattgacga taatttctgg caagccgaat
     1561 accgcgacta ccgccgtacc tccatctaag cctgcacccg gtagtgaagg ctaccgggct
     1621 atttccctct ccctttttca gatctcatcc atactgggta gtggcgaata aatctcattt
     1681 gcctcacctg ctatgcagaa catcatccga aaaggaggaa ctatgaagac tgcagttgtt
     1741 acgaaggatc atcatgttga cgttacggat aaaacactgc gctcactgaa acatggcgaa
     1801 gccctgctga aaatggagtg ttgtggtgta tgtcataccg atcttcatgt taagaatggc
     1861 gattttggtg acaaaaccgg cgtaattctg ggccatgaag gcatcggtgt ggtggcagaa
     1921 gttggcccag gtgtcacctc attaaaacca ggcgatcgtg ccagcgtggc gtggttttac
     1981 gaaggatgcg gtcattgcga atactgtaac agtggtaacg aaacgctctg ccgttcagtt
     2041 aaaaatgccg gatacagcgt tgatggcggg atggcggaag agtgcatcgt ggtcgccgat
     2101 tacgcggtaa aagtgccaga tggtctggac tcggcggcgc ccagcagcat tacctgtgcg
     2161 ggggtcacca cctacaaagc cgttaaactg tcaaaaattc gtcccggtca gtggattgct
     2221 atctacggtc ttggcggtct gggtaacctc gccctgcaat acgcaaagaa tgtctttaac
     2281 gccaaagtga tcgccattga tgtcaatgat gagcagttaa aactggcaac cgaaatggat
     2341 gcagatttag cgattaactc acgcaccgaa gacgccgcca aaattgtgca ggagaaaacc
     2401 ggtggcgctc acgctgcggt ggtaacagca gtagctaaag ctgcgtttaa ctcggcagtt
     2461 gatgctgtcc gtgcaggcgg tcgtgttgtg gctgtcggtc tgccgccgga gtctatgagc
     2521 ctggatatcc cacgtcttgt gctggatggc attgaagtgg tcggttcgct ggtcggcacg
     2581 cgccaggatc taaccgaagc cttccagttt gccgccgaag gcaaagtggt gccgaaagtc
     2641 gccctgcgtc cgttagcgga catcaacacc atctttaccg agatggaaga aggcaaaatc
     2701 cgtggccgta tggtgattga tttccgtcac taagaggtct ttgctgcgac tcccatgttc
     2761 gggtcgcagc atcgcacact ctccaacatg aaatggctaa atggattatt gttgcgtagg
     2821 aaggaacaat aatgatagtg ttcccagaga agtaacgcta ctgagagtaa agacactaat
     2881 gaaaatggcc aatcatcccc gcccggggga cattattcag gaatcactgg acgaacttaa
     2941 tgtcagcctg cgcgagtttg ccagagcaat ggaaattgcg ccctcaacgg caagccgatt
     3001 gctgaccgga aaagcagctt tgacgccaga aatggcaatt aaactttccg tggtgatcgg
     3061 cagttcgccg caaatgtggc tgaatctgca aaacgcctgg agtctggcag aggcagaaaa
     3121 aacggtggtt gtgtcgcgat tacgccgtct ggttacgcaa taaaaaatgg caccttataa
     3181 agcgccattt gaaactgatg acaaacgcaa cattgcctga tgcgctacgc ttatcaggcc
     3241 tacacgttca cttcaatcta ttgtatttga agggttttca tgccggataa ggcattcacg
     3301 acgcatccgg gatgaacaaa gcccacgttg tctacaatat gaaaatggcg ccttttacag
     3361 cgccattttc agcttaaagt tcaaaagaga ttatatccct tcttcactct cttttttcgc
     3421 ttctgccttc tcgatttcac gataccagcg cggatggtgt ttcttcgccc agcgacggct
     3481 caccttccct tcgatcatcc ctttaatcga ccctttcacc cagaatgcca tatacatatg
     3541 gatcaggatg gcgtggatca ggatgatacc cgcagccgcg tggatcagca ggctgtagcg
     3601 aacaacctgc atcgggaagt actgcgcaaa gtacggacgc cagataatca ccccggtcac
     3661 cagcagcacg aaaatcatgc tcatgatcga ccagaacatc atcttttgcc cggcgttgta
     3721 cttaccaaca tccgccactt tgtgctcatt gccttttaat acttcgacaa tgttcaacag
     3781 ccacggaata tctttcttat ccgggatgtt gtgatgcaca aaacgcacaa acataaacat
     3841 cagtgcgacg aaaatcgcaa tgccgaagaa ggggtgcaaa atgcgtccca tctgcggcgt
     3901 accgaaggtt tgcgtcagcc attgcagcgt cgggaagaag aacgaaatcc cggacagcgc
     3961 caccaggaag aagcaaatca ccacggtcca gtgacaggcg cgatcaataa atttggtgcg
     4021 cacaatcatt ttcgacttac tcatgatgat cctcctcgtc atcgtccact tccttattcg
     4081 ggccaatacc gatgtagtgg aaaatcaacc cggcaaaagt ggcaataaag ccagccgctg
     4141 ccagcggttt caacgcgcct ttccacaggc ttaccgaggt gtcgatcttc ggatctttcg
     4201 gcagaccgtg atacagctcc ggctgatcgg cgtgatgcag cacgtacata acgtgcgtac
     4261 caccgacccc ttccgggttg tagacgccag catgttcgta accacgcgct ttcagtttcg
     4321 ccacgcgctg ttccgccagc tccagcatct ccttcttggt gccgaagtgg atagccccgg
     4381 tcggacaggt tttcacacaa gccggttcct ggccgacgct gacgcgatcg acgcagagcg
     4441 tgcatttata tacccggtta tcctctttgt tgaggcgcgg aatattaaac ggacacccgg
     4501 caatgcagta accacagccg atgcagtttt ccgactggaa atcgacaatc ccgttagcgt
     4561 actgaatgat tgcaccagca gacgggcacg ccttcaggca gcccggatct tcacagtgca
     4621 tacagccgtc tttacggatc agccactcca gcttgccatt ctgttcggtt tcgctaaagc
     4681 gcatcaccgt ccaggacttg gcgctcagat cggcggggtt atcgtaaacc ccgacgcagt
     4741 gccccacttc atcacggatg tcgttccact ccgaacacgc cacctgacag gctttacagc
     4801 cgatacaggt ggaaacgtcg ataagtttcg cgacttctgc tttgtaatca cgcacctgag
     4861 aaggcggcgt gatggagtta gttgcggacc ttttgataat atcctgcgtt tccatagcca
     4921 tctgttcgcc cccttacgcc ttctcgatgt tgactaagaa cgctttatat tccggcgttt
     4981 gcgagtttgc atcaccgaca ttcggcgtca gagtgttagc gatataacct ttacgcgcga
     5041 caccctcaaa gccccagtgg attggaatac ccaccgtttc aacctgctga ccgtttacat
     5101 tcagcggttt cagacggcgc gttaccactg ccaccgcgcg gataaagcct cgcttgctgg
     5161 agacagtgac acgatcgcca ttattaatgc ctttcgccgc cgcctgcgtt tcgctgattt
     5221 ccacaaactg ttccggctga gcaattgcgt tgagcaacgc gtgcttggtc caggtgtgga
     5281 agtgctcggt cagacgatag gtcgtaccca catacgggaa ctgctctttt ttacccatcc
     5341 gcagcgcgtc ttgttcatac agacgaacaa ccgggttaga caccacgttc ggatgcagcg
     5401 ggttagtgcc cagcggcgtt tcaatcggct cgtagtgttc cgggaacgga ccttccgcca
     5461 ttttgttgat ggcaaacagg cgtcccatcc cttccggctg cataataaac ggcccggttg
     5521 gcgtacccgg tgcggcattg ccgaagtccg gaatatcgtt acccgtccac ttgctgccgt
     5581 tccactggat cagcatccgt ttcggatccc acggtttacc gttgatatcc gccgaagcac
     5641 ggttgtagag tacgcgacgg ttgagcggcc acgcccaggc ccatcccagc gtattcccca
     5701 gaccggacgg gtcggagtta tcgcggtttg ccatctggtt accctgctct gtccagctac
     5761 cggtgtagat ccagcaagaa gatgcggttg taccgtcatc acgcagatgc gcaaagctac
     5821 tcagcaactg acctttcttc gcaatcagca cgccattagc atcatagaga tcttccaacg
     5881 catagccgtt gttctcttta gctacttcgt cagattgcgg ttcgtgcggc tgcttgtagt
     5941 tccagctcat cttcatcagc ggttctacgc ctttaccacc ttcggcctgg tacaactcgc
     6001 gcagatgatg gtagataccc gccagaattt cgccgtcgtt acgcgcttcg cccggcgcgt
     6061 cctgaccttt ccagtgccac tgcaaccagc gaccggagtt agcgatagaa ccatcctctt
     6121 cagcaaagca ggtcgaaggc agacggaata cttcagtctg aatagacgcc ggatcgacat
     6181 cgttcgactc gccgtggttc tgccagaagg tagaggtttc agtcaccagc ggatcgataa
     6241 ccaccatgta cttcagtttg ctcaggcagc tcaccacttt gtttttatcc gggaaggacg
     6301 caaccgggtt aaagccctgg cagaaataac cggtaacttt gccttcatcc atcatgttga
     6361 aatacttgat gacgtcgtag gtctggtccc acttcggcag ccagtcatag ccccagttgt
     6421 tctctttctg cgcggcatcg ccatagaaag atttcatcag gctaacgaag aacttcggat
     6481 agttgctcca gtagttcacc tgatcagcca gcgtcgcttt cggcgtgttc gcttccagat
     6541 acgactgcaa atcaacctgt ttttctgacg gcagcgtcag ataacctggc aggctggtag
     6601 agagcagacc taagtcagtc agcccctgaa tgttggagtg accacgcaat gcgttcacgc
     6661 cgccaccggc catacccatg ttaccgagca gcaactggat catcgccata gtacggatgt
     6721 tctgcgcacc cacggtgtgc tgcgtccagc ccagcgcgta caggaaggtg gttgtgcgat
     6781 ccggtgcgct ggtggaggcc agcacttcac acactttcag gaagtcggct tttggcgtac
     6841 cacagatgtt ttcaacaacg tccggcgtgt agcgggaaac gtgcgctttc agcaggttcc
     6901 acacacagcg cggatgagtc agtgtttcat cgcgtttcgc atagccgttt tcatcgaact
     6961 gatagttcca ggacgatttg tcgtactggc gtttttcagc gtcataaccg ctgaacaggc
     7021 catcttcgaa agcaaaatca tcacgcacca gtaggctggc gttggtgtaa tgtttaacgt
     7081 attcggcgtt gattttgttg ttttcgatca ggtagcgcaa aatgccagac aggaacgtaa
     7141 tgtccgtacc ggaacgaata ggtgcgtaaa tatccgccac agaagcggta cgcgtaaaac
     7201 ggggatcgac aacgatcaag gttgcatcgt tgttgttttt cgcttccatc gcccagcgga
     7261 aaccgacggg atgcgcttca gcagcgttac cgcccattac cattacgacg ttagcgtttt
     7321 tgatatccac ccagtggttg gtcatagcac cgcgaccaaa tgttggagca agacttgcta
     7381 ccgttggtcc gtgtcagacg cgcgcctggt tgtctaccgc cagcatcccg agggagcggg
     7441 caaatttctg ggtcagcatc ccggtttcgt tgctggcacc ggaggcacac agcataccgg
     7501 tagaaagcca acggtttacc gttacgccct gctcgttctt ttcaataaag ttagcgtcac
     7561 ggtcagcttt catcagcttc gcaatacggg agaatgcttc ttcccagcta atgcgctgcc
     7621 atttgtcaga acctggcgca cgatatttcg ggtagcgcag acggttttca ctgttgacgt
     7681 aatccagcaa accggcccct ttcgggcaca gcgcaccacg gcttaccgga tgatccgggt
     7741 caccttcaat gtgataaatc gcttctctgg cgttttttgc gccatcaccc aggctataca
     7801 tcaatagccc gcaacctacg gaacagtatg tgcaggtgtt acggatctct ttagcgcgta
     7861 ataatttgta gtttcgcgcc tgagccagtg cttgcttcgg ggcaaagccc aatgccgcta
     7921 ccgttgttcc agccataccg cccgcgcaga ttttaaaaaa ttgtctgcga ctgacgtcca
     7981 ttgctttcct cttttttcag gggtattact gcgcgaggaa actaacagta aaattctgaa
     8041 tctttttgcg cgaaatcaaa aagaacacgt gtttgccaca aaataatccg cctgcgcaaa
     8101 ttgacctacc tcaatagcgg tagaaaaacg caccactgcc tgacaggcca gttaaaaaaa
     8161 tgctataaaa ttcagc
//
LOCUS       DAJTQY010000138         8022 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.138, whole genome shotgun sequence.
ACCESSION   DAJTQY010000138 DAJTQY010000000
VERSION     DAJTQY010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8022
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.138"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            <1..864
                     /locus_tag="OO349_003581"
     CDS             <1..864
                     /locus_tag="OO349_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coat protein"
                     /protein_id="HCR5868510.1"
                     /translation="GWDFVSDAERLMFSRELNRDMGISYFLNPDDYRKAGRNLVDGDI
                     FGRVPEEAYRNGTIQRQIAGFDEILRSPKLPAVTKSTATGVTVSGAQKFKPQAYTLDT
                     DGNKENVDNRVATVTVSSTTGFKRGDKISFIGVKFLSQMAKNVLTDDATFSITRVIDG
                     THIEITPKPIALDDASLTKEEKAYANVNTSLADTTPVNVLNVATTTANVFWADDSIRL
                     LSQPIPVTHELFAGMKTSSFSIPGIGVNGIFATQGDINTLSGKCRIAVWYSACAVRPE
                     AIGVGLPNQTA"
     gene            909..1133
                     /locus_tag="OO349_003582"
     CDS             909..1133
                     /locus_tag="OO349_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5868511.1"
                     /translation="MTHMIFRHGDMKKWKGVGYDFEIVKAEELQEYLDAGWFSHPDDL
                     LKDVAEPEEKQCKKPGRKPKAAADEPDNEG"
     gene            1111..1611
                     /locus_tag="OO349_003583"
     CDS             1111..1611
                     /locus_tag="OO349_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RmuC"
                     /protein_id="HCR5868512.1"
                     /translation="MNLTTKGDLVLAALRKLGVASNATLTDVEPQSMEDGVNDLEMMM
                     AEWLGGDASPGINVGYIFADADVAPDPGDEHGLSNNAINAVIFNLACRIAPDYALEAS
                     AKLITTARYGKERLVKLSAMDRAKAAKCKSGYPNRMPVGSGNQLAKWNGWNYFHRKEP
                     CDNGSE"
     gene            1611..3029
                     /locus_tag="OO349_003584"
     CDS             1611..3029
                     /locus_tag="OO349_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization protein gp10"
                     /protein_id="HCR5868513.1"
                     /translation="MPIQQLPLMKGVGKDFRNADYIDYLPVNMLATPKEILNSSGYLR
                     SFPGIAKRSDVNGVSRGVEYNMAQNAVYRVCGGKLYKGESEVGDVAGSGRVSMAHGRT
                     SQAVGVNGQLVEYRYDGTVKTVSNWPTDSGFTQYELGSVRDITRLRGRYAWSKDGTDS
                     WFITDLEDESHPDRYSAQYRAESQPDGIIGIGTWRDFIVCFGSSTIEYFSLTGATTVG
                     AALYVAQPSLMVQKGIAGTYCKTPFADSYAFISNPATGAPSVYIIDSGQVSPIASASI
                     EKILRSYTADELADGVMESLRFDAHELLIIHLPRHVLVYDASSSANGPQWCVLKTGLY
                     DDVYRAIDFVYEGNQITCGDKLESVTGKLQFDISSQYDKQQEHLLFTPLFKADNARVF
                     DLEVESSTGVAQYADRLFLSATTDCINYGREQMIEQNEPFVYDKRVLWKRVGRIRKNV
                     GFKLRVITKSPVTLSGAQIRIE"
     gene            3029..3826
                     /locus_tag="OO349_003585"
     CDS             3029..3826
                     /locus_tag="OO349_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5868514.1"
                     /translation="MADSNLNVPVIIQATRLDTSVLPRNIFSQSYLLYVIAQGTDVGN
                     VANKANEAGQGAYDAQVRNDEQDVILADHEQRISAAEATLVNHEERIRQAESTLQDHE
                     TRIAQNESDISSLDTRVQSLESQVSDHETRIDALEYATTRKKSEVVYSGVSVTIPTAP
                     TNLVSLLKTLTPSSGSLAPFFDTVNNKMVVFNENKTLLFKLSIVGTWPSGTANRSMQL
                     TFSGSVPDTLVSSRNAATTTDNILLATFFSVDKDGFLATTIKIIAEQ"
     gene            3826..4281
                     /locus_tag="OO349_003586"
     CDS             3826..4281
                     /locus_tag="OO349_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HCR5868515.1"
                     /translation="MITFKPTRNIDLIEAVGNHPDIIAGSNNGDGYDYKPECRYFEVN
                     VHGQFGGIVYYQEIQPLTLDCHAMYLPEIRGFSKEIGLAFWRYILTNTTVQCVTSFAA
                     RKFRHGQMYCAMIGLKRVGTIKKYFKGVDDVTFYSATREELIDFLNHGR"
     gene            4284..4892
                     /locus_tag="OO349_003587"
     CDS             4284..4892
                     /locus_tag="OO349_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HCR5868516.1"
                     /translation="MLYAFKLGRKLCGEEPYCPEKGGKGGSSDKSAKYAAEAQKYAAD
                     LQNQQWQTIMKNLAPFTPLAEQYVNQLQNLSSLEGQGQALNQYYNSKQYKDLAGQARY
                     QSLAAAEATGGLGSTATSNQLATIAPTLGQSWLSNQMSNYNNLANVGLGALQGQANAG
                     QTYANNMSSIAQQSAALASLLGTSTPWGAGIGAGIGLLGSLF"
     gene            4902..6233
                     /locus_tag="OO349_003588"
     CDS             4902..6233
                     /locus_tag="OO349_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HCR5868517.1"
                     /translation="MATWQGTNGGLLAGIGGVNSNAPSVNDIGNTLQLIRQNNDIERS
                     GANNVGLTALQGLSGIAGVFQQEKQAQRQKEFQQAYANAYASGDRGALRQLATQYPDQ
                     IESVRKGMGFIDEEQRNSIGTLAAGARLASSSPEAMQSWLQNNAGELARVGVNPHDVA
                     QMYQQNPRQFGEFVDHLGMNSLGPEKYFDLQDKMQGRQVTMRGQDLDSQTAARNQAIT
                     MRGQDIQANLGQQRINLDAETNRINNENKRLDRMLSAETNDLKRQEIQSRIAANNQQL
                     QQKQQALNDGYKDGINTLTTSMFTLNDIVSSPSLKSITGLRGVIPNVPGSQAADTQAR
                     LDTFKSQAYLTAVQAMRGMGALSDAEGKKLDQAVGSLQNSQSEESFRRNAGVILNTLN
                     QKRNEAVGKYVQQNGIKRVEAPQASIDYLKQHPELSIDFINRYGYLPSLGQ"
     gene            6234..7964
                     /locus_tag="OO349_003589"
     CDS             6234..7964
                     /locus_tag="OO349_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HCR5868518.1"
                     /translation="MANYRDLLEQAGARYGVPEGLMTALGAKESSYNPAAVSSAGAVG
                     LTQVMPGTWRDMGYTDEQMQNPEYQADAGARYLAKMYQQFGNWRDALQAYHDGPGNVM
                     KAKRGEYTPGPEGRGYVDDRFAQWAGDPVTDSTVEQRATSAKVHPQQDPNNPFAQLEV
                     QSSEQVSASGVQSDPNNPFAQIEQQAASQQPPQPVSSVAPKPVQQQTVNQANNAPTRE
                     EPSLMQQAGDWLTGGQSAGQIAEQAGRGLVNIPFDVLQGGASLINAISQGLGGPKVLD
                     DVYRPVDRPTDPYAQAGESIGGYLVPGAGVAGNMVIGSLADAANQRGDFAENAAINAG
                     LSIATHGLINGVTRGVRGASNIISGNKTSAQRATTAPTETSPFSGDAAAATNPAVHAA
                     EARVAQGVPMTPATKNPEEVVRTVAAQKRPNLASSLDELDINPQAEVLESAERLNVDS
                     LLPSHFSGNEQYKAVEQAIKSRAGSALQVQENEAIRQLAQGAGEIIDRVSGAKDALGM
                     SDKFIDTVNGRMSALMKRSDQLYRNVEKAMPAGAKIDAPSTRSMLKQVAEDLGGMKNL
                     DPIEKRVEVA"
BASE COUNT         2126 a         1962 c         2098 g         1836 t
ORIGIN      
        1 ggctgggatt ttgtgtctga tgcagagcga ctgatgttct cccgtgagct aaaccgcgac
       61 atgggcatca gttacttcct gaaccctgac gattaccgca aagcaggccg caacctggta
      121 gatggtgaca tcttcgggcg cgttcctgaa gaagcatatc gaaacggtac tattcagcgt
      181 cagattgctg gctttgatga aattcttcgc tcaccgaaac ttccggcagt taccaagtca
      241 accgcgactg gtgtaactgt ttctggtgcg cagaagttta agccgcaggc atacaccctt
      301 gataccgatg gtaacaaaga gaacgtcgac aaccgtgttg caacggtgac cgtatcctcc
      361 accaccggat ttaagcgcgg cgacaaaatc agcttcattg gtgtgaagtt cctgtctcag
      421 atggcgaaga atgtgctgac tgatgacgcg actttctcaa tcacccgtgt gatcgatggt
      481 actcacatcg aaatcacgcc gaagccgatt gcgctggatg acgcgtcact gacaaaagaa
      541 gagaaggctt acgctaacgt aaacacctct cttgctgata ccactccggt aaacgttctg
      601 aacgtggcaa caaccaccgc taacgtgttc tgggctgatg actcaatccg cctgctgtct
      661 cagccgatcc cggtaaccca tgaactgttt gctggcatga aaacgtcttc cttcagcatt
      721 ccaggtattg gtgttaacgg catcttcgca acgcagggtg atatcaacac tctgtctggt
      781 aagtgccgta ttgctgtgtg gtattcagca tgtgctgtac gaccagaggc aattggtgtt
      841 ggtctgccta accagaccgc gtgataacca gagggagctt cggctcccgt ttttatctgg
      901 agacaagcat gacacacatg atctttcgtc atggcgacat gaagaagtgg aaaggcgttg
      961 gctacgactt tgaaatcgtg aaagccgaag agcttcagga atatctggat gctggctggt
     1021 tttcacatcc tgatgacctt ttgaaggatg ttgcagagcc agaagaaaaa cagtgtaaaa
     1081 agcctggtcg aaaacctaag gcggcagcag atgaacctga caacgaaggg tgatttagtt
     1141 cttgcggcat tacgtaagct cggtgttgca tcaaatgcca cgttaaccga tgtcgaaccg
     1201 cagtctatgg aagacggcgt caacgacctt gaaatgatga tggctgaatg gcttggcggt
     1261 gatgcgtcac ctggtatcaa cgttggctac atttttgctg atgcagatgt tgctccggat
     1321 ccgggcgatg agcacggttt atcaaataac gctatcaatg ccgtcatttt caaccttgcc
     1381 tgccgaattg ctccagatta tgcgctggaa gcgtctgcaa aacttataac cactgccaga
     1441 tacgggaaag agcgactcgt caaactgtct gcaatggaca gggcaaaagc agctaaatgt
     1501 aagtccggtt atccaaaccg tatgcctgtt ggtagtggta accagttggc gaagtggaat
     1561 ggttggaatt acttccaccg aaaggaacct tgcgataacg ggagcgaata atgcctattc
     1621 agcaacttcc gcttatgaaa ggtgtcggca aagacttccg aaacgccgac tatatcgact
     1681 atctgccagt gaatatgctg gctacaccca aagaaatcct gaacagcagc ggatatcttc
     1741 gctcattccc gggcattgcc aaacgttctg acgtgaacgg tgtatctcgc ggcgtcgagt
     1801 acaacatggc gcagaatgct gtttatcgcg tgtgtggcgg caagctgtac aaaggagaaa
     1861 gtgaagtcgg tgatgttgcc ggaagtggtc gtgtatcaat ggcgcatggt cggacatcac
     1921 aggcggtagg cgttaatggt caactggtcg agtatcgcta tgatggcacg gtcaaaaccg
     1981 tctcaaactg gcctacagac agtggattca ctcagtatga gttaggttca gttcgcgaca
     2041 ttacgcgctt acgtgggcgt tatgcgtggt caaaagacgg aactgattca tggtttatca
     2101 ctgaccttga agacgaatct catcctgacc gttacagcgc acaatatcgt gccgagtctc
     2161 agcctgacgg catcatcggt atcgggacat ggcgagactt catcgtctgc tttggttcat
     2221 cgactattga atatttctcc ctgactggcg caaccaccgt tggtgccgct ttgtatgttg
     2281 cacaaccatc gctgatggtg cagaaaggca ttgccggaac ttactgcaaa acgccattcg
     2341 ctgattcgta tgcgtttatc agcaatccgg caacgggtgc gccgtctgtg tatatcatcg
     2401 actccggtca ggtgtcacca attgccagcg cgagcattga gaaaatcctc cgctcctaca
     2461 ctgctgatga actggctgat ggtgtgatgg aatcgctgcg atttgatgct catgagttgc
     2521 tgattatcca ccttccgcgt cacgtcctcg tgtacgacgc atcttcaagc gccaatggcc
     2581 cacaatggtg tgtgctgaaa acaggtctgt atgacgatgt gtaccgcgct atcgacttcg
     2641 tttacgaagg caatcagata acgtgcggcg ataagctgga atctgttacc gggaaactgc
     2701 aattcgatat cagcagccag tacgacaagc aacaggaaca cctgctgttt actccactct
     2761 tcaaagcgga taacgccaga gtgttcgacc ttgaggttga atcgtcaact ggcgttgcgc
     2821 agtacgccga ccgccttttt ctctctgcaa ccactgactg catcaattac gggcgtgagc
     2881 agatgattga gcagaatgaa ccgttcgttt acgacaagcg tgttttgtgg aagcgagtag
     2941 ggcgcatcag gaaaaatgtc ggcttcaaat tgcgcgttat cacgaagtca cctgtcactc
     3001 tgtctggcgc tcagataagg attgagtaat ggctgattcg aatctcaatg tgccggtaat
     3061 catccaagct acgcggctcg atacatcagt ccttccacgc aatatcttct cgcagtcgta
     3121 tctgctttac gttatcgcac agggcactga tgttggtaac gtggctaaca aagccaacga
     3181 ggccggacag ggcgcttatg atgcacaggt caggaacgat gagcaggatg tgattctcgc
     3241 tgaccatgag cagcgaattt ctgctgcgga agcaacgctt gttaatcatg aggagcgaat
     3301 cagacaggca gaatcaactc ttcaggacca tgaaacacga atagctcaga atgaaagcga
     3361 tatttcgtcg cttgacacaa gagttcagtc gctggagtcg caggtttcag accatgaaac
     3421 gcgcatcgat gctctggagt atgcaaccac acgcaagaag tcagaggttg tttactctgg
     3481 tgtatctgta accatcccga cagcgccgac caaccttgtt agcctgctga aaacgctcac
     3541 accgtcatcc ggctcgttgg caccattctt cgacaccgtt aacaacaaga tggttgtgtt
     3601 caacgagaac aaaaccctgc tcttcaagtt gtcgattgtc ggcacgtggc ccagcggaac
     3661 cgccaacagg tcaatgcagc taaccttttc cggctctgtt cctgacacac tggtcagcag
     3721 tcgtaatgcg gcgacaacaa ccgacaacat cctgttagct acgttcttca gcgtggataa
     3781 agacggcttt cttgccacaa ccatcaagat aatcgcggag cagtgatgat tacattcaaa
     3841 ccaacgcgaa acatcgacct gatagaagca gtcggaaatc accctgacat tatcgccggg
     3901 agcaacaacg gtgatggata cgactacaaa cctgaatgcc gttactttga ggtgaacgtg
     3961 cacggtcagt ttggcggcat tgtttactat caggagattc agccgctgac attagattgc
     4021 cacgccatgt acctgccaga gattcgcggc ttcagcaagg aaattgggct ggcgttctgg
     4081 cgatacattc tgactaacac caccgttcag tgcgtaacat cgttcgctgc acgcaaattc
     4141 cgacacggtc agatgtactg cgcaatgatt ggccttaagc gtgtaggaac catcaagaaa
     4201 tacttcaaag gcgtggatga cgtgactttt tacagcgcca cacgcgaaga actaatcgac
     4261 ttcctgaatc acgggagata gccatgttat atgcatttaa gctgggcaga aaactgtgcg
     4321 gcgaggaacc ttattgccct gaaaaaggcg ggaaaggtgg cagttctgat aaaagcgcaa
     4381 agtatgcagc agaagcccag aagtatgctg cagacctgca aaatcagcag tggcagacga
     4441 tcatgaaaaa ccttgctccg ttcacgcctc ttgcggagca gtatgttaac cagcttcaga
     4501 acctttccag tttagaaggt caggggcagg cacttaatca gtattacaac tctaagcagt
     4561 ataaagacct tgcagggcag gcgcgttacc agagtcttgc tgctgcggag gcgacgggtg
     4621 gacttggttc gacagccaca agcaatcaac tggctacgat cgcgccgaca ctcggtcagt
     4681 cttggttatc aaatcagatg agcaattaca acaatctggc aaacgttggg cttggtgcgc
     4741 tgcaaggtca ggcaaacgcc gggcagacgt acgccaacaa catgagcagc attgcacagc
     4801 aaagcgcagc tcttgccagc cttttgggaa catcaacgcc ttggggcgct ggtattggtg
     4861 ctggtatcgg attgcttggc tcgttgtttt aaggggtaat catggctact tggcaaggaa
     4921 caaacggcgg attgttggct ggtatcggcg gcgtcaactc aaacgctccg agcgtaaatg
     4981 acatcggcaa tacgcttcag cttatcaggc agaacaatga tattgagcgt tcaggcgcta
     5041 acaatgttgg gctgactgct ttgcaaggtc tttcaggtat tgcgggtgtt tttcagcagg
     5101 aaaagcaggc tcagcggcag aaagaatttc agcaggcgta cgctaatgct tatgcgtctg
     5161 gtgatcgcgg tgctttgcgt cagttggcta ctcaatatcc agaccagatt gaatccgttc
     5221 gtaaaggcat gggattcatt gatgaagagc agcgtaattc tatcggcacc ttagcggctg
     5281 gcgcacgcct tgcgtcatcg tctccagaag caatgcaatc atggctgcaa aacaacgccg
     5341 gtgagttagc tcgtgttggc gttaatcctc atgacgtcgc tcagatgtac caacagaacc
     5401 cgcggcagtt cggcgaattt gtcgatcacc tggggatgaa cagtctcggg cccgaaaaat
     5461 actttgacct acaggataaa atgcagggtc gccaggttac catgcgcggt caggatctgg
     5521 attcgcaaac cgccgctcgg aatcaggcaa tcacaatgcg cggacaagat atccaggcga
     5581 atttaggtca gcagcgcatt aatctggacg cagaaacaaa ccgcattaac aacgaaaata
     5641 agcgccttga ccggatgcta tcagcagaaa ctaacgacct gaagcgccag gaaatacaga
     5701 gccgcatagc agccaacaac cagcagttgc agcagaagca gcaagcgcta aatgatggct
     5761 acaaagacgg catcaacacc ctcacaacca gcatgttcac tctgaacgat atcgttagtt
     5821 ctccttcact taagagcatt acaggcttac gtggagtaat ccccaacgtt ccaggctcac
     5881 aggctgcaga cactcaggca cgacttgata cctttaaatc acaagcatac ctgacagcgg
     5941 ttcaggccat gcgaggcatg ggcgcacttt ctgatgccga gggcaaaaag ctcgaccagg
     6001 ctgttggttc gctgcagaac tcgcagagcg aggagtcctt tcgtcgcaac gctggcgtca
     6061 tcctgaacac gctcaaccag aagcgtaatg aggcggttgg taagtacgtt cagcaaaacg
     6121 gtatcaagcg agtggaagcg cctcaggctt ctatagatta cctgaagcag caccccgagc
     6181 tgtcaatcga cttcattaat cgctacggat atcttccatc tttggggcag taaatggcta
     6241 attaccgtga tttgttagag caggctggcg cacgttacgg tgtgccagaa gggttgatga
     6301 ctgcactggg tgccaaggag tcttcttaca accctgccgc agtaagctcc gccggggctg
     6361 taggattgac tcaggtcatg cctgggacat ggcgtgatat gggttatacc gatgagcaaa
     6421 tgcaaaaccc cgaatatcag gctgacgctg gcgcgcgcta tctggcaaag atgtaccagc
     6481 agtttggtaa ctggcgtgac gctcttcagg cttatcacga cggtcccggc aacgttatga
     6541 aggcaaagcg tggtgaatat acgccaggac ctgaaggccg cggttacgtt gatgatcgct
     6601 ttgctcaatg ggctggtgac ccggtgacag actcaacagt cgaacagcgc gccacctctg
     6661 caaaggtaca tcctcagcaa gaccctaaca acccgtttgc acaactggaa gtacagtcat
     6721 ccgaacaagt atcggcatca ggtgtgcagt cagacccaaa taatccattt gctcagattg
     6781 agcagcaggc agccagtcag cagccacctc aacccgtaag ttctgtcgca ccgaaacctg
     6841 ttcagcaaca aacagttaat caggccaata atgcaccaac acgggaagaa ccatcattga
     6901 tgcaacaagc tggcgattgg ctcactggtg gtcaaagtgc agggcaaatt gcagaacagg
     6961 ctggtcgtgg tctggtaaac ataccatttg acgtattgca gggtggcgca agtctgatta
     7021 atgcaatcag tcaggggctt ggggggccaa aagtattgga tgatgtttat cgtccagtag
     7081 acagaccgac agacccctac gcgcaagctg gagagtcaat aggcggttat cttgttcctg
     7141 gagcaggtgt agctggaaac atggtcattg gttctctcgc tgacgcggcg aatcaacggg
     7201 gtgattttgc cgaaaatgcc gctattaatg ccggacttag cattgctacg catggcctga
     7261 taaatggcgt tacccgtggt gttcgtggtg catcaaatat aattagtggc aataaaacat
     7321 ctgcacagag agcgaccact gcgccaacag aaacatcacc attctccggt gatgccgctg
     7381 cagcaacaaa tcctgcggtt catgccgcag aggcaagagt agcacaaggt gtaccaatga
     7441 cgcctgcgac gaagaaccca gaggaagtcg ttcgcacagt agcagcacaa aaaaggccaa
     7501 atctcgcttc atcgcttgat gaactagata tcaatcctca ggctgaagtt ctggagtctg
     7561 ctgaaaggct taatgttgat tcattactcc cttcacactt ttccgggaac gagcaataca
     7621 aggcagttga gcaagcaatc aagtcccgtg cgggttctgc tctacaggtg caggaaaatg
     7681 aagcaatcag gcagctagca cagggcgcgg gtgagataat tgatcgcgtt tccggtgcaa
     7741 aagatgctct tggtatgagc gacaagttta ttgatacggt caatggaaga atgtctgcgc
     7801 tgatgaaacg aagcgaccag ctttatcgca atgttgaaaa ggcgatgcct gcaggtgcaa
     7861 aaattgatgc gccatcaaca aggtcaatgc tcaaacaggt ggcagaagat cttggcggga
     7921 tgaaaaacct tgaccctatt gaaaagagag ttgaggtagc ctgagtttaa cggacactcc
     7981 ttcctgaaat agaatggcat cagaaggagc taataatgag ca
//
LOCUS       DAJTQY010000139         7916 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.139, whole genome shotgun sequence.
ACCESSION   DAJTQY010000139 DAJTQY010000000
VERSION     DAJTQY010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7916
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.139"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            237..740
                     /gene="dps"
                     /locus_tag="OO349_003590"
                     /gene_synonym="pexB"
     CDS             237..740
                     /gene="dps"
                     /locus_tag="OO349_003590"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HCR5868519.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            1144..1890
                     /gene="glnH"
                     /locus_tag="OO349_003591"
     CDS             1144..1890
                     /gene="glnH"
                     /locus_tag="OO349_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HCR5868520.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFSTEPK"
     gene            2029..2688
                     /gene="glnP"
                     /locus_tag="OO349_003592"
     CDS             2029..2688
                     /gene="glnP"
                     /locus_tag="OO349_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HCR5868521.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            2685..3407
                     /gene="glnQ"
                     /locus_tag="OO349_003593"
     CDS             2685..3407
                     /gene="glnQ"
                     /locus_tag="OO349_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HCR5868522.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALGNVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     NPQVLIKNPPSQRLQEFLQHVS"
     gene            3668..5892
                     /gene="ybiO"
                     /locus_tag="OO349_003594"
                     /pseudo
     CDS             3668..5892
                     /gene="ybiO"
                     /locus_tag="OO349_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
     gene            complement(5889..6815)
                     /gene="rlmF"
                     /locus_tag="OO349_003595"
     CDS             complement(5889..6815)
                     /gene="rlmF"
                     /locus_tag="OO349_003595"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HCR5868523.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVNFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            7091..7351
                     /locus_tag="OO349_003596"
     CDS             7091..7351
                     /locus_tag="OO349_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR5868524.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMLGTATIYK"
     gene            7616..>7916
                     /locus_tag="OO349_003597"
     CDS             7616..>7916
                     /locus_tag="OO349_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HCR5868525.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQANADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPG"
BASE COUNT         1934 a         2025 c         1978 g         1979 t
ORIGIN      
        1 cgtatttata tatcgaatta tgcaaaagca tatttattcc gaaaattcct ggcgagcaga
       61 taaataagaa ttgttcttat caatatatct aactcattga atctttatta gttttgtttt
      121 tcacgcttgt taccactatt agtgtgatag gaacagccag aatagcggaa cacatagccg
      181 gtgctatact tagtctcgtt aattactggg acataacatc aagaggatat gaaattatga
      241 gtaccgctaa attagttaaa tcaaaagcga ccaatctgct ttatacccgc aacgatgtct
      301 ccgacagcga gaaaaaagca acagtagagt tgctgaatcg ccaggttatc cagtttattg
      361 atctttcttt gattaccaaa caagcgcact ggaacatgcg cggcgctaac ttcattgccg
      421 tacatgaaat gctggatggc ttccgcaccg cactgatcga tcatctggat accatggcag
      481 aacgtgccgt gcagctgggc ggtgtagctc tggggaccac tcaagttatc aacagcaaaa
      541 ccccgctgaa aagttacccg ctggacatcc acaacgttca ggatcacctg aaagaactgg
      601 ctgaccgtta cgcaatcgtc gctaatgacg tacgcaaagc gattggcgaa gcgaaagatg
      661 acgacaccgc agatatcctg accgccgcgt ctcgcgacct ggataaattc ctgtggttta
      721 tcgagtctaa catcgaataa atccatcgct gatggtgcag aactttagta cccgataaaa
      781 gcggcttcct gacaggaggc cgttttgttt tgcagcccac ctcaacgcac ttatttagtg
      841 catccatctg ctatccccag ctgattaagt aaattttttg tatccacatc atcacacaat
      901 cgttacataa agattgtttt ttcatcaggt tttacgctaa ataatcactg tgttgagtgc
      961 acaattttag cgcaccagat tggtgcccca gaatggtgca tcttcagggt attgccctat
     1021 aaatcgtgca tcacgttttt gccgcatctc gaaaaatcaa tgagttgcaa aattggcacg
     1081 attttttcat atatgtgaat gtcacgcagg ggatcgtccc gtggatagaa aaaaggaaat
     1141 gctatgaagt ctgtattaaa agtttcactg gctgcactga ccctggcttt tgcggtttct
     1201 tctcatgccg cggataaaaa attagttgtc gcgacggata ccgccttcgt tccgtttgaa
     1261 tttaaacagg gcgataaata tgtgggcttt gacgttgatc tgtgggctgc catcgctaaa
     1321 gagctgaagc tggattacga actgaagccg atggatttca gtgggatcat tccggcactg
     1381 caaaccaaaa acgtcgatct ggcgctggcg ggcattacca tcaccgacga gcgtaaaaaa
     1441 gcgatcgatt tctctgacgg ctactacaaa agcggcctgt tagtgatggt gaaagctaac
     1501 aataacgatg tgaaaagcgt gaaagatctc gacgggaaag tggttgctgt gaagagcggt
     1561 actggctccg ttgattacgc gaaagcaaac atcaaaacta aagatctgcg tcagttcccg
     1621 aacatcgata acgcctatat ggaactgggc accaaccgcg cagacgccgt tctgcacgat
     1681 acgccaaaca ttctgtactt catcaaaacc gccggtaacg gtcagttcaa agcggtaggt
     1741 gactctctgg aagcgcagca atacggtatt gcgttcccga aaggtagcga cgagctgcgt
     1801 gacaaagtca acggcgcgtt gaaaaccctg cgcgagaacg gaacttacaa cgaaatctac
     1861 aaaaaatggt tcagtactga accgaaataa taacgctaca cctgtaaagc gcactggcag
     1921 ttccctctcc cccctgggga gaggattagg gtgaggggcg caaacccgct ccggggccat
     1981 taattaccct gaatttgatt atttacacca cggtaacagg aacaacatat gcagtttgac
     2041 tggagtgcca tctggcctgc cattccgctt ctgattgaag gtgccaaaat gaccctgtgg
     2101 atttcggtcc tcggtctggc tggcggtctg gtaatcggat tgctggcagg ttttgcacgc
     2161 accttcggtg gttggatagc caaccacgtc gcgctggtct ttattgaagt gatccgcggc
     2221 acacctatcg tcgtccaggt gatgtttatc tatttcgccc tgccgatggc gtttaacgac
     2281 ttacgcatcg acccatttac tgcggcggtg gtcaccatca tgatcaactc cggcgcgtac
     2341 attgcggaaa tcacgcgtgg tgcggtgctg tctatccaca aaggttttcg tgaagcagga
     2401 ctggcgctcg gtctttcacg ttgggaaacc attcgctacg tcattttacc gctggcactg
     2461 cgtcgtatgc tgccgccgct gggtaaccag tggatcatca gcattaaaga cacctcgctg
     2521 tttattgtta tcggcgtggc ggaactgacc cgtcaggggc aagaaattat tgccggtaac
     2581 ttccgcgccc ttgagatctg gagcgccgtg gcggtgttct atctgattat taccctggtg
     2641 ctgagcttta ttctgcgtcg tctggaaaga aggatgaaaa tcctgtgatt gaatttaaaa
     2701 acgtctccaa gcactttggc ccaacccagg tgctgcacaa tatcgatttg aacattgccc
     2761 agggcgaagt cgtggtgatt atcgggccgt ccggttccgg taaatcgacc ctgctgcgct
     2821 gcatcaacaa actggaagaa atcacctccg gcgatctgat tgtcgatggc ctgaaggtca
     2881 acgatccgaa agttgacgag cgcctgattc gccaggaagc aggtatggtg ttccagcagt
     2941 tttacctctt cccgcatctg acggcgctgg gaaacgtcat gtttggtccg ctacgcgtgc
     3001 gtggcgcgaa caaagaagag gcggaaaaac tggcgcgtga gctgctggcg aaagtcggtc
     3061 tggcagaacg tgcacatcac tacccttccg aactttctgg tggtcaacag cagcgtgtgg
     3121 cgattgcccg cgcgctggcg gtgaagccga aaatgatgct gtttgatgaa ccgacttccg
     3181 ctcttgaccc ggaactgcgc catgaagtgc tgaaggttat gcaggatctg gctgaagaag
     3241 ggatgacgat ggtgatcgtg acccacgaaa tcggttttgc cgagaaagta gcttcgcggc
     3301 tgatctttat cgacaaaggc cggattgcgg aagatggcaa tccgcaggtg ttgatcaaga
     3361 acccgccgag ccagcgcttg caggaatttt tgcagcacgt ctcttaataa gacacattgc
     3421 ctgatgcgct acgcttatca ggcctacagg atatctggca acttattaaa attgcatgaa
     3481 cttgtaggac ggataaggcg ttcacgccgc atccggcaaa aaagcccgca cgttgtcagc
     3541 aacctgctta atatcccttc ctccctttca cccgaaaggg aggcacacca gattcctctc
     3601 atttaaaatc gcccctcccc cagcatctat acttatcttt ttgctctgtt ttctcactgg
     3661 aggagtcatg cggtggatcc tgttcatcct cttctgcctg ctgggcgcac ctgcccacgc
     3721 ggtatccata cccggcgtta caaccacaac gacaacggac tcaacgactg aaccggcccc
     3781 ggaaccggat atcgaacaaa aaaagcggcc tatggcgcac tggcggatgt gctggataac
     3841 gacacctcgc gtaaagagtt gatcgaccag ttgcgtactg tcgccgcgac gccccctgcg
     3901 gaaccggtcc cgaagatcgt gccgccgacg ctggttgaag agcaaaccgt gctgcaaaag
     3961 gtcaccgaag tcagccgcca ttatggtgaa gccctttccg cccgcttcgg gcaactttat
     4021 cgcaatatca ccggctcccc gcataagcca tttaatccac aaaccttcag caatgcgctg
     4081 agccattttt caatgttagc ggtattagtg tttggttttt actggctgat tcgcctgtgc
     4141 gcactgccgc tatatcgcaa aatgggtcag tgggcgaggc aaaaaaatcg tgagcgcagc
     4201 aactggttgc agcttccggc gatgattatt ggggcgttta ttatcgacct gctgttactg
     4261 gccctgacat tatttgtcgg ccaggtatta agcgacaacc tgaatgcagg cagtcgcacc
     4321 atcgctttcc aacaaagttt gtttctcaac gcctttgccc tgattgaatt tttcaaagcc
     4381 gtactacgcc tgattttttg cccaaacgtg gcggagctgc gcccgttcac gattcaggac
     4441 gagagcgccc gttactgggg tcgtcgcctg agctggttaa gcagcctgat tggctatggc
     4501 ctgattgtgg ccgtgccgat tatctctaat caggtgaatg tacagatagg tgcgctggcg
     4561 aacgtcatca ttatgctgtg catgaccgtc tgggcgttgt acctgatctt ccgaaataaa
     4621 aaagagatta cccagcatat gctcaacttt gcggagcatt cgctggcttt tttcagcctg
     4681 tttatccgcg cctttgcgct ggtgtggcac tggctggcaa gcgcctattt tatcatgctg
     4741 tttttctttt cgttgttcga tccgggcaac agtctgaaat ttatgatggg tgcaacggtg
     4801 cgcagcctgg cgattattgg tatcgcagcg tttgtttccg gtatgttttc ccgctggctg
     4861 gcgaaaacca tcaccctctc gccacatact cagcgtaact atccggagct acaaaaacgg
     4921 ctgaatggct ggctttcggc ggcgctgaaa acggcgcgta ttctgaccgt ctgcgtggca
     4981 gtaatgttgc tgttgagcgc atggggattg ttcgatttct ggaactggct gcaaaacggc
     5041 gcggggcaga aaaccgtaga tatcctgatc cgcatcgcac tcattctttt cttctcggcg
     5101 gttggctgga cggtgctcgc cagtttgatc gaaaaccggc tggcttcgga tattcatggc
     5161 cgcccgctac ccagcgcccg tacgcgtacc ctgctgacgc tgttccgtaa cgcgctggcg
     5221 gtgattatca gtaccatcac catcatgatt gtgttgtcgg aaatcggcgt caatatcgcg
     5281 ccattgctgg caggtgccgg ggcattaggt ctggctatct cgtttggttc gcaaacgctg
     5341 gtaaaagata ttatcaccgg ggtatttatt cagtttgaaa acggcatgaa cactggagat
     5401 ttggtgacta tcgggccgtt gaccggcaca gtggaacgga tgtcgattcg ctccgtgggc
     5461 gtgcgccagg ataccggggc gtatcacatc attccgtggt cttcgataac cacctttgct
     5521 aacttcgtcc gcggtattgg ttcggtagtg gcaaactacg atgttgatcg ccatgaagat
     5581 gctgataaag ccaatcaggc actgaaagat gcggtaacgg aattgatgga aaacgaagaa
     5641 attcgcgggc tgattattgg tgaaccgaac tttgccggga ttgtcggctt aagcaatacc
     5701 gcgtttacac tgcatgtttc gttcaccacg ctgccactca aacagtggac ggtacgcttt
     5761 gccctcgaca gccaggtgaa aaaacatttc gacctggcgg gcgttcgcgc gccagtgcag
     5821 acttatcagg tgctgcctgc tccgggcgcg accccggctg aaccgctgcc gccgggggag
     5881 ccaacgcttt aacgttggcg attaacaaaa cggcggcgct gctcgtcgtt cataaaggtc
     5941 caggcaataa agcgactctg cttttgcccc tgggccatct ctttttttac caccttcacc
     6001 gcgcccacgt cggtcagagc acgatacaac ggcggtaagt tttcaccacg agataccagc
     6061 gaggtaaacc acatcacctg cttcgcgaag cctttgctct cttcaatcat ctttttgata
     6121 aagttgactt caccgccttc acaccacaac tcctgttgct gaccaccaaa gttcagtgca
     6181 tcgtctttgt tcagccccag gttacggcgt ttacgctcac tacctgcccg cgccgcagcg
     6241 gcggaatcgt ggaatggcgg gttacacaag gtcgcgtcgt attgctcgtt tttgtggatg
     6301 atgccgttaa aaatcgcccc actctctttt tgccgacgca gacgaatggc gcggttaagc
     6361 cccggattag cactgataat cgcctgcgca ctgcttaacg cctggctgct ggtttcacta
     6421 ccggtaaaac gccagccata ttcatgtacg ccaatcagcg gataaataca gttcgcacca
     6481 acgccgatat ccagaatgct ggcattcgct ggaattgttc cgcttgcttc agccagtaaa
     6541 tcggcaaggt gatgaatata atccgcccgg cccggtactg gtgggcagag aaaaccgtcg
     6601 gggatatccc agttcgctac ggcgtaaaaa tgggccagca acgccttatt gagcgccttc
     6661 accgccagcg gattggcaaa gtctacgctt tgctcccctg cgggtgtaag cgtgaggaat
     6721 tgcctgagtt caggattgac ctgacaaagc gtggcgagat cgtagcggct gtgatgacgg
     6781 ttgcgcggat gcaaccccgg tttctgggcg gacatagcat tctcctttaa gcagcggcgt
     6841 aagatacccg cataacgcga cagggtaaat atctggctgc gtttatcatt ccccaaaacc
     6901 ttaacatggc tatggtttat ggcaattcat cataaaaatc agcattatgc atgtcattga
     6961 tttgtcttat tttgtaaaaa tatatcgttc aaaaaataag catatctctg tcaatttttt
     7021 gcgtcatttg catcgcgcag gcgacctaca cttatcaggc actacccgtt gtatttatga
     7081 ggatgtcatt atgaaaaagt gcctcacact actgattgcc accgtcctga gcggaatctc
     7141 tctcacggct tatgccgcgc aaccgatgag taacctggac agcggtcagc tacggcccgc
     7201 cggtaccgtt tcggcaactg gcgcatcaaa cctaagcgat ctggaggata aactggcaga
     7261 aaaagcgcgc gaacaaggcg cgaaaggtta tgtcattaat tccgcaggcg gaaatgacca
     7321 gatgttaggt actgcaacca tctacaaata accccaccaa cgtaactgca ttcgcctctg
     7381 gatgcagttt tcgcctgttt tgtaatcaaa aaacatcaaa tatgaacgca atgtaaataa
     7441 atgtatttct ttttcgcgca atgggtgata gaaaatcgca tcaagtgata atgcttatca
     7501 aaattactat cacttgcacg aacactatca cgggattaac agtggcatcg catccgcaga
     7561 gatgctttct cgtggcagtg aaaatttcaa catataagaa aaagtcacct gcaaaatgga
     7621 aaacaatcgc aatttccctg ccagacaatt tcattcgctc acgttctttg ccggtctttg
     7681 tattggtatt acgcctgtgg cacaggcact cgccgccgaa gggcaagcta acgcggatga
     7741 cacgctggtt gtcgaagcat caacgccttc gctttatgcg ccacaacaat ctgccgatcc
     7801 gaaattctcg cgtccggtag cggatactac ccgcacgatg acggtgattt ctgaacaagt
     7861 gattaaagat cagggcgcaa ccaaccttac cgacgcgctc aaaaacgtcc ccggcg
//
LOCUS       DAJTQY010000140         7910 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.140, whole genome shotgun sequence.
ACCESSION   DAJTQY010000140 DAJTQY010000000
VERSION     DAJTQY010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7910
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.140"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(123..953)
                     /locus_tag="OO349_003598"
     CDS             complement(123..953)
                     /locus_tag="OO349_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-1"
                     /protein_id="HCR5868526.1"
                     /translation="MKNTIHINFAIFLIIANIIYSSASASTDISTVASPLFEGTEGCF
                     LLYDASTNAEIAQFNKAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGM
                     EIWNSNHTPKTWMQFSVVWVSQEITQKIGLNKIKNYLKDFDYGNQDFSGDKERNNGLT
                     EAWLESSLKISPEEQIQFLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGA
                     GFTANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL
                     "
     gene            1163..2176
                     /gene="intI1"
                     /locus_tag="OO349_003599"
     CDS             1163..2176
                     /gene="intI1"
                     /locus_tag="OO349_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HCR5868527.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            2379..2729
                     /locus_tag="OO349_003600"
     CDS             2379..2729
                     /locus_tag="OO349_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="HCR5868528.1"
                     /translation="MEVVAEGVETPDCLAWLRQAGCDTVQGFLFARPMPAAAFVGFVN
                     QWRNTTMNANEPSTSCCVCCKEIPLDAAFTPEGAEYVEHFCGLECYQRFQARASTATE
                     TSVKPDACDSPPSG"
     gene            2855..3415
                     /locus_tag="OO349_003601"
     CDS             2855..3415
                     /locus_tag="OO349_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR5868529.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
                     GEQKTKLAREFGISRETLYQYLRTDQ"
     gene            3418..6384
                     /locus_tag="OO349_003602"
     CDS             3418..6384
                     /locus_tag="OO349_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs3 family transposase"
                     /protein_id="HCR5868530.1"
                     /translation="MPRRSILSAAERESLLALPDSKDDLIRHYTFNDTDLSIIRQRRG
                     PANRLGFAVQLCYLRFPGVILGVDELPFPPLLKLVADQLKVGVESWNEYGQREQTRRE
                     HLSELQTVFGFRPFTMSHYRQAVQMLTELAMQTDKGIVLASALIGHLRRQSVILPALN
                     AVERASAEAITRANRRIYDALAEPLADAHRRRLDDLLKRRDNGKTTWLAWLRQSPAKP
                     NSRHMLEHIERLKAWQALDLPTGIERLVHQNRLLKIAREGGQMTPADLAKFEPQRRYA
                     TLVALATEGMATVTDEIIDLHDRILGKLFNAAKNKHQQQFQASGKAINAKVRLYGRIG
                     QALIDAKQSGRDAFAAIEAVMSWDSFAESVTEAQKLAQPDDFDFLHRIGESYATLRRY
                     APEFLAVLKLRAAPAAKNVLDAIEVLRGMNTDNARKLPADAPTGFIKPRWQKLVMTDA
                     GIDRRYYELCALSELKNSLRSGDIWVQGSRQFKDFEDYLVPPEKFTSLKQSSELPLAV
                     ATDCEQYLHERLTLLEAQLATVNRMAAANDLPDAIITESGLKITPLDAAVPDTAQALI
                     DQTAMVLPHVKITELLLEVDEWTGFTRHFTHLKSGDLAKDKNLLLTTILADAINLGLT
                     KMAESCPGTTYAKLAWLQAWHTRDETYSTALAELVNAQFRHPFAGHWGDGTTSSSDGQ
                     NFRTASKAKSTGHINPKYGSSPGRTFYTHISDQYAPFHTKVVNVGLRDSTYVLDGLLY
                     HESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGDTKLYIPKGDAAYDALKP
                     MIGGTLNIKHVRAHWDEILRLATSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRI
                     ERTLFILDWLQSVELRRRVHAGLNKGEARNALARAVFFNRLGEIRDRSFEQQRYRASG
                     LNLVTAAIVLWNTVYLERAAHALRGNGHAVDDSLLQYLSPLGWEHINLTGDYLWRSSA
                     KIGAGKFRPLRPLQPA"
     gene            6451..6828
                     /locus_tag="OO349_003603"
     CDS             6451..6828
                     /locus_tag="OO349_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5868531.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSG
                     QLHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGG
                     RINDHIVNYYLHGESLSKLASGI"
     gene            complement(7029..7688)
                     /locus_tag="OO349_003604"
     CDS             complement(7029..7688)
                     /locus_tag="OO349_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HCR5868532.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
BASE COUNT         1753 a         2241 c         2133 g         1783 t
ORIGIN      
        1 cttccctcat gatgtttaac gtttgaaata aggggcgccg agcgccagcg aggggagcca
       61 aaagcttgct tttggccgtc ccgacttgat tgaagggttg ggcgattttg ccattagatt
      121 ttttataaat ttagtgtgtt tagaatggtg atcgcatttt tcttggcttt tatgcttgat
      181 gttaaattcg accccaagtt tcctgtaagt gcggacacaa aaacatattt atgtcctgat
      241 ttgcttataa taaacccttc aaaccatccg ttttgtaagg ttctatttgc tgtgaatcct
      301 gcaccagttt tcccatacag ttttgtacta ttatccagat cttgtagata catgttctct
      361 atggtgtttt ctatggctga gtttttaact gggagattgt gattaataat tttacgcagg
      421 aattgaattt gttcttctgg tgaaattttt aagctacttt cgagccatgc ttctgttaat
      481 ccgttgtttc tttctttatc tccagagaag tcttgatttc cataatcaaa atctttgaga
      541 taattcttga ttttatttaa tccaattttt tgggttattt cttgcgaaac ccaaacaaca
      601 gaaaattgca tccacgtctt tggtgtatga ttgctgttcc agatctccat tcctttgggg
      661 gttttatccc atttgaatat ggttttctga tctattattt ccgcatcaaa tgccataagt
      721 gataatgcga tcttgaaagt tgaatctggt gccatttgcg ttgcacactt tgctttattg
      781 aattgagcaa tttcagcgtt tgtggatgca tcgtaaagta aaaaacaacc ttcagttcct
      841 tcaaataatg gagatgcaac agtagagata tctgttgatg cactggcgct gctgtagata
      901 atatttgcaa ttattaaaaa aatagcgaag ttgatatgta ttgtgttttt cataataagt
      961 attggtttgg taaagggctt aattttaacg gctaacaatt aatgaggctc cgggttcgcc
     1021 caactttgtt ttagggcgac tgccctgctg cgtaacatcg ttgctgctcc ataacatcaa
     1081 acatcgaccc acggcgtaac gcgcttgctg cttggatgcc cgaggcatag actgtacccc
     1141 aaaaaaacag tcataacaag ccatgaaaac cgccactgcg ccgttaccac cgctgcgttc
     1201 ggtcaaggtt ctggaccagt tgcgtgagcg catacgctac ttgcattaca gcttacgaac
     1261 cgaacaggct tatgtccact gggttcgtgc cttcatccgt ttccacggtg tgcgtcaccc
     1321 ggcaaccttg ggcagcagcg aagtcgaggc atttctgtcc tggctggcga acgagcgcaa
     1381 ggtttcggtc tccacgcatc gtcaggcatt ggcggccttg ctgttcttct acggcaaggt
     1441 gctgtgcacg gatctgccct ggcttcagga gatcggaaga cctcggccgt cgcggcgctt
     1501 gccggtggtg ctgaccccgg atgaagtggt tcgcatcctc ggttttctgg aaggcgagca
     1561 tcgtttgttc gcccagcttc tgtatggaac gggcatgcgg atcagtgagg gtttgcaact
     1621 gcgggtcaag gatctggatt tcgatcacgg cacgatcatc gtgcgggagg gcaagggctc
     1681 caaggatcgg gccttgatgt tacccgagag cttggcaccc agcctgcgcg agcagctgtc
     1741 gcgtgcacgg gcatggtggc tgaaggacca ggccgagggc cgcagcggcg ttgcgcttcc
     1801 cgacgccctt gagcggaagt atccgcgcgc cgggcattcc tggccgtggt tctgggtttt
     1861 tgcgcagcac acgcattcga ccgatccacg gagcggtgtc gtgcgtcgcc atcacatgta
     1921 tgaccagacc tttcagcgcg ccttcaaacg tgccgtagaa caagcaggca tcacgaagcc
     1981 cgccacaccg cacaccctcc gccactcgtt cgcgacggcc ttgctccgca gcggttacga
     2041 cattcgaacc gtgcaggatc tgctcggcca ttccgacgtc tctacgacga tgatttacac
     2101 gcatgtgctg aaagttggcg gtgccggagt gcgctcaccg cttgatgcgc tgccgcccct
     2161 cactagtgag aggtagggca gcgcaagtca atcctggcgg attcactacc cctgcgcgaa
     2221 ggccatcggt gccgcatcga acggccggtt gcggaaagtc ctccctgcgt ccgctgatgg
     2281 ccggcagcag cccgtcgttg cctgatggat ccaacccctc cgctgctata gtgcagtcgg
     2341 cttctgacgt tcagtgcagc cgtcttctga aaacgacaat ggaggtggta gccgagggtg
     2401 tggaaacacc cgactgcctt gcgtggttgc ggcaggcggg ttgcgacacg gtgcagggtt
     2461 tcctgttcgc caggccgatg ccggcggcgg ccttcgtcgg cttcgtcaac caatggagga
     2521 acaccaccat gaacgccaat gaaccgagca ccagttgctg cgtgtgctgc aaggaaatcc
     2581 cgctcgatgc cgccttcacg ccggaagggg ccgagtacgt ggagcatttc tgcgggctgg
     2641 agtgctatca gcgcttccag gcgcgggcca gcactgcgac cgaaaccagc gtcaaaccgg
     2701 acgcttgtga ttcgccgccg tcaggttgag gcatacccta acctgatgtc agatgccatg
     2761 tgtaaattgc gtcaggatag gattgaattt tgaatttatt gacatatctc gttgaaggtc
     2821 atagagtctt ccctgacatt ttgcagggaa ttccatgact ggacagcgca ttgggtatat
     2881 cagggtcagc accttcgacc agaacccgga acggcaactg gaaggcgtca aggttgatcg
     2941 cgcttttagc gacaaggcat ccggcaagga tgtcaagcgt ccgcaactgg aagcgctgat
     3001 aagcttcgcc cgcaccggcg acaccgtggt ggtgcatagc atggatcgcc tggcgcgcaa
     3061 tctcgatgat ttgcgccgga tcgtgcaaac gctgacacaa cgcggcgtgc atatcgaatt
     3121 cgtcaaggaa cacctcagtt ttactggcga agactctccg atggcgaacc tgatgctctc
     3181 ggtgatgggc gcgttcgccg agttcgagcg cgccctgatc cgcgagcgtc agcgcgaggg
     3241 tattgcgctc gccaagcaac gcggggctta ccgtggcagg aagaaatccc tgtcgtctga
     3301 gcgtattgcc gaactgcgcc aacgtgtcga ggctggcgag caaaagacca agcttgctcg
     3361 tgaattcgga atcagtcgcg aaaccctgta tcaatacttg agaacggatc agtaaatatg
     3421 ccacgtcgtt ccatcctgtc cgccgccgag cgggaaagcc tgctggcgtt gccggactcc
     3481 aaggacgacc tgatccgaca ttacacattc aacgataccg acctctcgat catccgacag
     3541 cggcgcgggc cagccaatcg gctgggcttc gcggtgcagc tctgttacct gcgctttccc
     3601 ggcgtcatcc tgggcgtcga tgaactaccg ttcccgccct tgttgaagct ggtcgccgac
     3661 cagctcaagg tcggcgtcga aagctggaac gagtacggcc agcgggagca gacccggcgc
     3721 gagcacctga gcgagctgca aaccgtgttc ggtttccggc ccttcaccat gagccattac
     3781 cggcaggccg tccagatgct gaccgagctg gcgatgcaaa ccgacaaagg catcgtgctg
     3841 gccagcgcct tgatcgggca cctgcggcgg cagtcggtca ttctgcccgc cctcaacgcc
     3901 gtcgagcggg cgagtgccga ggcgatcacc cgtgctaacc ggcgcatcta cgacgccttg
     3961 gccgaaccac tggcggacgc gcatcgccgc cgcctcgacg atctgctcaa gcgccgggac
     4021 aacggcaaga cgacctggtt ggcttggttg cgccagtctc cggccaagcc aaattcgcgg
     4081 catatgctgg aacacatcga acgcctcaag gcatggcagg cactcgatct gcctaccggc
     4141 atcgagcggc tggttcacca gaaccgcctg ctcaagattg cccgcgaggg cggccagatg
     4201 acacccgccg acctggccaa attcgagccg caacggcgct acgccactct cgtggcgctg
     4261 gccaccgagg gcatggccac cgtcaccgac gaaatcatcg acctgcacga ccgcatcctg
     4321 ggtaagctgt ttaacgctgc caagaataag catcagcagc agttccaggc gtcaggcaag
     4381 gccatcaacg ccaaggtacg tctgtacggg cgcatcggtc aggcgctgat cgacgccaag
     4441 caatcaggcc gcgatgcgtt tgccgccatc gaggccgtca tgtcctggga ttcctttgcc
     4501 gagagcgtca ccgaggcgca gaagctcgcg caacccgatg acttcgattt cctgcatcgc
     4561 atcggcgaga gctacgccac cctgcgccgc tatgcaccgg aattccttgc cgtgctcaag
     4621 ctgcgggccg cgcccgccgc caaaaacgtg cttgatgcca ttgaggtgct gcgcggcatg
     4681 aacaccgaca acgcccgcaa gctgccagcc gatgcaccga ccggcttcat caagccgcgc
     4741 tggcagaaac tggtgatgac cgacgccggc atcgaccggc gctactacga actgtgcgcg
     4801 ctgtccgagt tgaagaactc cctgcgctcg ggcgacatct gggtgcaggg ttcacgccag
     4861 ttcaaggact tcgaggacta cctggtaccg cccgagaagt tcaccagcct caagcagtcc
     4921 agcgaattgc cgctggccgt ggccaccgac tgcgaacaat atctgcatga gcggctgacg
     4981 ctgctggaag cacaacttgc caccgtcaac cgcatggcgg cagccaacga cctgccggat
     5041 gccatcatca ccgagtcggg cttgaagatc acgccgctgg atgcggcggt gcccgacacc
     5101 gcgcaggcgc tgatagacca gacagccatg gtcctgccgc acgtcaagat caccgaactg
     5161 ctgctcgaag tcgatgagtg gacgggcttc acccggcact tcacgcactt gaaatcgggc
     5221 gatctggcca aggacaagaa cctgttgttg accacgatcc tggccgacgc gatcaacctg
     5281 ggcctgacca agatggccga gtcctgcccc ggcacgacct acgcgaagct cgcttggctg
     5341 caagcctggc atacccgcga cgaaacgtac tcgacagcgt tggctgaact ggtcaacgct
     5401 cagtttcggc atccctttgc cgggcactgg ggcgatggca ccacatcatc atcggacgga
     5461 cagaatttcc gaaccgctag caaggcaaag agcacggggc acatcaaccc aaaatatggc
     5521 agcagcccag gacggacttt ctacacccac atctccgacc aatatgcgcc attccacacc
     5581 aaggtggtca atgtcggcct gcgcgactca acctacgtgc tcgacggcct gctgtaccac
     5641 gaatccgacc tgcggatcga ggagcactac accgacacgg cgggcttcac cgatcacgtc
     5701 ttcgccctga tgcacctctt gggcttccgc ttcgcgccgc gcatccgcga cctgggcgac
     5761 accaagctct acatcccgaa gggcgatgcc gcctatgacg cgctcaagcc gatgatcggc
     5821 ggcacgctca acatcaagca cgtccgcgcc cattgggacg aaatcctgcg gctggccacc
     5881 tcgatcaagc agggcacggt gacggcctcg ctgatgctca ggaaactcgg cagctacccg
     5941 cgccagaacg gcttggccgt cgcgctgcgc gagttgggcc gcatcgagcg cacgctgttc
     6001 atcctcgact ggctgcaaag cgtcgagcta cgccgccgcg tgcatgccgg gctgaacaag
     6061 ggcgaggcgc gcaatgcgct ggcccgtgcc gtgttcttca accgccttgg tgaaatccgt
     6121 gaccgcagtt tcgagcagca gcgctaccgg gccagcggcc tcaacctggt gacggcggcc
     6181 atcgtgctgt ggaacacggt ctacctggag cgtgcggcgc atgcgttgcg cggcaatggt
     6241 catgccgtcg atgactcgct attgcagtac ctgtcgccac tcggctggga gcacatcaac
     6301 ctgaccggtg attacctatg gcgcagcagc gccaagatcg gcgcggggaa gttcaggccg
     6361 ctacggcctc tgcaaccggc ttagcgtgct ttattttccg ttttctgaga cgacccctat
     6421 tatggcgaag gtatcattca ggaagcgttg atgaaggatt atctctgtaa aaagttattc
     6481 aaccggcttt ccggtaccct ggtgatcaga gcgcgctgcg gcaataacat tactggcctg
     6541 gcatgttgca atattcttta tccctcgccc cgatacagcg gtcagctgca tattaaagag
     6601 ctgtatgttt ctcagtgcga tcgaaataag ggcacgggta aagcgataat gcgctttata
     6661 gcccggcttg cgcttgaaca ggaatgcctt agccttagct ggaacgctga aaaatccaac
     6721 cccggcgcta accgttttta tcaggctctg ggaggcagaa taaatgatca tatcgtcaat
     6781 tattacctcc acggggagag cctgagcaaa ctggcctcag gcatttgaga agcacacggt
     6841 cacactgctt ccggtagtca ataaaccggt aaaccagcaa tagacataag cggctattta
     6901 acgaccctgc cctgaaccga cgaccgggtc gaatttgctt tcgaatttct gccattcatc
     6961 cgcttattat cacttattca ggcgtagcaa ccaggcgttt aagggcacca ataactgcct
     7021 taaaaaaatt acgccccgcc ctgccactca tcgcagtact gttgtaattc attaagcatt
     7081 ctgccgacat ggaagccatc acaaacggca tgatgaacct gaatcgccag cggcatcagc
     7141 accttgtcgc cttgcgtata atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc
     7201 atattggcca cgtttaaatc aaaactggtg aaactcaccc agggattggc tgagacgaaa
     7261 aacatattct caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca
     7321 tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat
     7381 gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc
     7441 accagctcac cgtctttcat tgccatacgg aattccggat gagcattcat caggcgggca
     7501 agaatgtgaa taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag
     7561 gccgtaatat ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc
     7621 tcaaaatgtt ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt
     7681 ttctccattt tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt
     7741 agtgatctta tttcattatg gtgaaagttg gaacctctta cgtgccgatc aacgtctcat
     7801 tttcgccaaa agttggccca gggcttcccg gtatcaacag ggacaccagg atttatttat
     7861 tctgcgaagt gatcttccgt cacaggtatt tattcggcgc aaagtgcgtc
//
LOCUS       DAJTQY010000141         7897 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.141, whole genome shotgun sequence.
ACCESSION   DAJTQY010000141 DAJTQY010000000
VERSION     DAJTQY010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7897
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.141"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(<1..941)
                     /gene="hprS"
                     /locus_tag="OO349_003605"
     CDS             complement(<1..941)
                     /gene="hprS"
                     /locus_tag="OO349_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HCR5868533.1"
                     /translation="MKRLSITVRLTLLFIFLQSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHSDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIILCSVLSPLLIRTGLREIKKLSGVTEALNYNDSRVPVEVNALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQIRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLN"
     gene            complement(941..1612)
                     /gene="hprR"
                     /locus_tag="OO349_003606"
     CDS             complement(941..1612)
                     /gene="hprR"
                     /locus_tag="OO349_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HCR5868534.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLKMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            1745..2158
                     /gene="hiuH"
                     /locus_tag="OO349_003607"
     CDS             1745..2158
                     /gene="hiuH"
                     /locus_tag="OO349_003607"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HCR5868535.1"
                     /translation="MLKRYLVLSVATAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            2267..3271
                     /gene="msrP"
                     /locus_tag="OO349_003608"
                     /pseudo
     CDS             2267..3271
                     /gene="msrP"
                     /locus_tag="OO349_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
     gene            3272..3907
                     /gene="msrQ"
                     /locus_tag="OO349_003609"
     CDS             3272..3907
                     /gene="msrQ"
                     /locus_tag="OO349_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HCR5868536.1"
                     /translation="MRLTAKQVIWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLAALLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQSMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLLSLFNRLRKQAHNKLSL"
     gene            4165..4815
                     /gene="zinT"
                     /locus_tag="OO349_003610"
     CDS             4165..4815
                     /gene="zinT"
                     /locus_tag="OO349_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HCR5868537.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDTNVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYRKGYP
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            complement(5893..6162)
                     /locus_tag="OO349_003611"
     CDS             complement(5893..6162)
                     /locus_tag="OO349_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR5868538.1"
                     /translation="MLPQHSDIEIAWYASIQQEPNGWKTVTTQFYIQGFSEHIAPLQD
                     AVDLEIATEEENSLLEAWKKYRVLLNRVDTSTAPDIEWPEEPDTM"
     gene            6219..6887
                     /locus_tag="OO349_003612"
     CDS             6219..6887
                     /locus_tag="OO349_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR5868539.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            7203..>7897
                     /locus_tag="OO349_003613"
     CDS             7203..>7897
                     /locus_tag="OO349_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR5868540.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPDFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTLLQQTA"
BASE COUNT         2284 a         1770 c         1587 g         2256 t
ORIGIN      
        1 ttattgagag aaagcgaatc cagtttcacc aaaacattgt ttttgtctgc cctggcaaga
       61 aacagtatat tctctgttaa ccgcgaaata ttttccagct cttcaatgtt tccggcaatt
      121 gttttttgat attcagcgat acttctgatt tgactgagcg taacctgatt ctgacccagt
      181 aatgcattaa ttggcgttct aagttcatga gcgagatcgt cagcaaattg actaaggcgc
      241 tcaaaatctt tgactaaagc atgatgcatt ttattcaacg cctgcccaag aggttttagt
      301 tctctcggta atgcgttaac ctcaaccggc actcggctat cgttataatt cagcgcttcc
      361 gttacgccac tcaacttttt gatctctcgc aaccccgtcc tgattaacaa cggacttaat
      421 actgagcaca gtataatggc gacaatgcaa attataatgc tattaatttt atactgttca
      481 agcatattat gtctggctga agccaattta gccacagtaa ccgttaatga tggcgaaact
      541 tcatcaatgt taattcgtaa agcatctatc tctgtatcat taataatgct tctgtaaatg
      601 cctgctgcgc tgattgtctc actagcaggt atgttattta acatgtcatc actgacattt
      661 gtccggttaa caattttatt gatgccatca ctatgaatga tcaaaatatc ctgactaaca
      721 tccatcatcc ggttaaagta cacaggtaac gtatctggat ttaccccatc aattaacaac
      781 tgcttgatct gcgctgtccg gttaatgagt gttgtatcat cgcgccattt caactcactt
      841 gccaggccat tatagagagt ccatacaatt ccagcgccag caacagactg cagaaatata
      901 aaaagcaagg ttaaacggac ggttatagat aatcttttca ttattttttt accgctacga
      961 atgaatagcc catcccccgg attgtggcga ttagcttttc aggaaaagga tcatcaactt
     1021 ttgcgcggag cctgcgaatg gcgacatcca ccgtattggt atcactatca aagttgattc
     1081 cccaaatttc actcgcaata accgttctgg gtataatctc gccagctctg gaggccagta
     1141 gccaaagtaa ctgaaactcc ttgcgcgtca gtgtaatact gatattgtcc ctgctgacac
     1201 tttgactgac agagtccatt tttaagccac tgatttctaa tgttgaattc agagcgtgat
     1261 gttgccttaa ttgtgcccga acccttgcta gcaactcaga aaatgaaaaa ggttttacca
     1321 gataatcatt tgccccactg tccaaccctc tgactctgtc atcgacagaa tcccttgcag
     1381 taaggcaaat aacaggggtt tgctttgccg ttcttaacgt ttgtaagatc tgccagccat
     1441 ccatacccgg aagcataata tccagaatga tcaatgcata atcatccttc agcgcaagat
     1501 aaagcccatc tctgccatca gaaacggcat caatgacata acccgcttcg gaaagcccct
     1561 gcgttaccca ttcctgggtc ctttgattat cttcaataag tagaatcttc atgaaaataa
     1621 tatgccataa atcatatgtt atagaaacaa cctggttcat tacaaaattg taatgctgct
     1681 gtaaggttac cctggacgtt ttttcgctat cctcaaaact cattcacatg acaaggatat
     1741 aaacatgtta aagcgttatt tagtactctc ggtagcaacg gcagcatttt cattaccttc
     1801 tttggtttat gccgcacaac aaaacattct tagcgtgcac attttgaacc agcaaacagg
     1861 aaaacccgct gccgacgtga cagtcactct tgaaaagaaa gcggataacg gctggttaca
     1921 acttaatacc gccaaaacag ataaggatgg acgaattaag gcactgtggc ccgagcaaac
     1981 tgcgactact ggcgattacc gtgtcgtatt taaaaccggg gactatttca agaaacaaaa
     2041 tcttgaaagt ttcttccctg agattcccgt tgaatttcat attaataaag tgaacgagca
     2101 ttatcatgtg cctttgttac tcagccaata tgggtattca acctatcgtg gcagttaatg
     2161 cgtaaatatg taaaagctga tctctgctac tgttagggag gtgactccct gacagattaa
     2221 cagtaaacag ctcttgttgg ttaacgacaa aaaagtgtga tggcttatga aaaagaagca
     2281 atttttaaaa gaaccagatg ttacggccga gtcggtattc tttatgaagc gtcggcaggt
     2341 gttaaaagca ctgggcatca gcgcagctgc attttctttg cctcacgctg cgcatgccga
     2401 tctgcttagc tggtttaaag ggaacgatcg tccgcccgcc cccgccggaa aaccgctgga
     2461 gttcagcaag cctgccgcct agcaaaataa cctgccactg acgccagcag ataaagtctc
     2521 cggttataac aacttctatg aattcgggct ggataaagac gatcccgcgg ctaatgctgg
     2581 tagcctgaaa accgatccat ggacactgaa aatcagcggc gaagtggcaa aaccattgac
     2641 cctcgatcat gatgatttaa cccgtcgctt cccgctggaa gagcgtattt atcgtatgcg
     2701 ctgcgtggaa gcgtggtcga tggtggtgcc gtggattggt tttccgctac acaaattact
     2761 ggcgcttgcc gaacccacca gcaatgcgaa gtatgtcgct ttcgaaacga tttatgcgcc
     2821 ggaacagatg cctggccagc aggaccgctt tatcggcggc gggctgaaat atccttatgt
     2881 cgaaggattg cgtctcgacg aagcaatgca tccgctcaca ctgatgaccg taggtgttta
     2941 tggcaaggcg ttaccaccgc aaaatggcgc gccggtgcga ctgattgtgc cgtggaaata
     3001 tggctttaaa gggattaaat cgatcgtcag tattaagctg acccgcgagc gtccgccaac
     3061 cacctggaat ctggcagcgc ctgacgaata cggtttttac gccaacgtta atccgcatgt
     3121 tgatcacccg cgctggtcac aggctaccga acgatttatt ggttcaggcg gcattctcga
     3181 tgttcagcgc cagccaacac tactgtttaa tggttacgcc gaccaggtgg catcgctgta
     3241 tcgtggcctg gatttgcggg agaatttcta aatgcgtctg acagcaaaac aagtgatatg
     3301 gctgaaagtt tgcctgcatc ttgccggatt gttgccgttt ctctggctgg tctgggcgat
     3361 caatcacggt ggactgggtg ccgatccggt gaaagatatt cagcatttta ccggtcgcac
     3421 tgcactgaaa tttttgcttg cggccttgtt aatcacacct ctggcacgct acgcaaaaca
     3481 gccgttattg atacgcactc gccgcctgtt aggcttatgg tgctttgcct gggcgacact
     3541 gcatttaacc agttacgcat tgctggagtt gggcgtgaat aatctggcgt tattaggtaa
     3601 ggagttaatt acccggcctt atttaacgtt aggaattatc agctgggtta ttttgcttgc
     3661 tttagcgttc acttcaaccc agtcgatgca gcgaaaactc ggcaaacact ggcaacagtt
     3721 gcataacttc gtctatcttg tcgcgatcct ggccccaata cattatctgt ggtcagtgaa
     3781 gattatttca ccgcagccac tcatctacgc tgggctggct gtactgcttt tagccttacg
     3841 gtataagaag ttgctttccc ttttcaaccg gttacgcaag caggcccaca acaaattatc
     3901 tctataactt catattgccg acaaacttcg cgttatcccg gccggatgcg gcgcgagcgc
     3961 cctatccggc ccacacagat cgcatcaagt tatgtttcgt ttgtcatcaa cctttcatca
     4021 ccagaataat ttattttatt tcaataagat agttcaatac acctgagaat accatgctct
     4081 catttcctaa tgagttgttg cattttttca tatgttacaa tataacatta cacataatgt
     4141 acattaactc tggaggaaat tgttttggcg attcgtcttc ataaactggc tgttgcttta
     4201 ggtgtcttta ttgttagcgc tcctgccttt tcgcatggtc atcattctca tggcaaaccc
     4261 ttaacagagg tcgaacaaaa agctgctaat ggtgtttttg atgataccaa tgtacaaaac
     4321 cgaacgctca gtgactggga tggagtctgg caatccgttt accctttact gcaaagtggc
     4381 aaacttgatc ccgtctttca gaagaaagcg gatgcagata aaaccaaaac atttgctgaa
     4441 attaaagatt attatcgcaa aggttatccg acagatatcg aaatgattgg cattgaggac
     4501 ggcattgtcg aattccatag aaataatgaa acaacatcct gtaaatatga ttacgatgga
     4561 tacaaaatac tcacctataa atcaggcaag aaaggcgttc gttacttatt cgaatgtaaa
     4621 gatcctgaaa gcaaagcccc taaatatata caatttagcg atcatataat tgcaccacga
     4681 aaatcatctc attttcacat ttttatgggt aatgattccc agcaatcttt attgaatgaa
     4741 atggaaaact ggccaacgta ttatccctat cagttgagta gcgaagaagt ggtcgaggag
     4801 atgatgtctc attgattttt ttgcgctgac ttcagttatt taacagaaca gtgcctggta
     4861 gtaacaacaa taatttacca agacaaataa gtaagacaaa ctcagacacg ctcatacttg
     4921 agtcgtgtct tttttattat gtctgtagaa aaaaactgag aaaaaggcta acaataaata
     4981 cacaactcag ccatacagac tggcagcgca aatgtttcca ggagcaagtc atctgtcatg
     5041 gtaaagctaa ccagaaatat aggttaattt tggtgtttaa acctttttta cttgttagta
     5101 tgatacaaag gctttcaaaa aagctgggaa attcaacaac tcttggaaat tatggacttt
     5161 gtcccgcggc tttaatctga cggccgcgtt ccttttttgg ctcaattatt gaccccttct
     5221 ctacgcaact tcagttcccg ccagcaactt tgcggcagtt tcataggatc aatgtctaaa
     5281 agaataatgg tgaccgataa gaaaacgact gaataactgt agattttcgc tcgaaacctt
     5341 cctgtcagct ccatagcgaa tcaagcgctg aatgtcacag tatcgaacag aaaacagtga
     5401 cgatctaacc cttcaagaat attctacgat tgttctgttt aggaaaagca aggcgggaag
     5461 tcgggagata agtcattgat aaaatggcgg agagaggggg atttgaaccc ccggtagagt
     5521 tgcccctact ccggttttcg agaccgatga tacagagtcg aaaaatcaaa gagttaaaaa
     5581 tttttcttgg aaaaaatgtc ttaaaatcgt acacgaaaaa tcaatgaatt acataacatt
     5641 aaagaagaat attccaagcc ttcttgagtc caccacctgt tttcaggtgt cattcagacg
     5701 tgatttgccc cagattggga aagcacgctt ttttctcagc ttattccgtt ttgcctgtgt
     5761 gttgaataat tccactgcag ttatataaaa tcggtaacgg ctggaaatca ttcaatactc
     5821 gcactatcga aagtttacca gccaaccgca gcacgtcctg catacgtcgt gtctgcggtt
     5881 tttctttttc gcttacattg tgtctggttc ttccggccac tcaatatcag gtgcagttga
     5941 tgtatcaaca cggttcagca acacccgata cttcttccag gcttccagca acgagttttc
     6001 ttcctccgtt gcgatctcca gatctacagc atcctgaagt ggcgcaatat gctcactgaa
     6061 tccctggatg tagaactgtg tggtgacggt cttccagcca ttcggctcct gctgtatcga
     6121 agcataccag gctatttcaa tatcgctatg ctgcggcagc atttaacccc ttgtaattca
     6181 tcgccataat tgatttaatt cacaaataaa actataatat ggtgaaatta atgaaaaaaa
     6241 acacagatga tggggctaaa atttacacac cacttaccct aaagctttat gactggtggg
     6301 ttttgggagt atcaaatcgg cttgcatggg gatgtcctac aaaggaacac cttcttccac
     6361 actttctgga acatttaggt aacaaccatc tggatattgg tgttggaact gggttttacc
     6421 ttactcacgt acctgagagt agtctgatat ctttaatgga tttgaacgaa gctagcctga
     6481 acgcggcatc tacaagggct ggggaatcaa aaattaaaca taaaattagc catgatgttt
     6541 ttgaacctta tcctgcggcg ttacatggtc aatttgattc catttccatg ttttaccttc
     6601 ttcactgcct gcctggaaat atatctacaa aaagctgtgt aatacgcaat gcggcgcagg
     6661 ccttaactga tgatggaact ctatacggag ccacaattct tggcgatgga gttgtgcaca
     6721 atagcttcgg tcaaaaactg atgcgcattt acaatcagaa aggcatcttt tcaaacacaa
     6781 aagattccga agaaggctta acacatatac tctcagagca tttcgagaat gttaaaacca
     6841 aggttcaagg tactgtagta atgttttccg cttcagggaa aaaatagcat ccaaccgcag
     6901 cacgttcttg cttaagacgt gctacggttt cctttacgat ttggggttgc gactttacct
     6961 ctattgataa tgcattccgg cagaacgttc aaatatgaac gtacgatatt taactaaccg
     7021 aaaaacaaaa tatagcacag gcgagacatt taccatctaa gaaaatagta tcgttttttt
     7081 acagttaaat caacatcact tccttaaaat gaaaacaata aatttcaatc ctgaaacatt
     7141 ttatttcagc ttattattcc ccagtatcac attttatcag gatatccttc tgcgaggtta
     7201 ttatgcttcc tgtaaataat ccccccctat ccactggaaa cgtctctttt tacagaacta
     7261 catcaatcga caatgttcac aataattatc tctccgaatg ggttgaatgg actaaaaaca
     7321 gcatttccgg agaaaacagg gaaactgctt ttacccggct ccaattatgt ctggagaaca
     7381 gtgaaacatc gttggactta tcttgtttag gtctcagatc tctaccacga ttgcctgaca
     7441 atcttgatga aattaatgta agcaataacc aactatcaat gctccccgag ctaccaaggg
     7501 cattgaaaga gctgaatgca agcagtaatc aattatctgc acttcctgaa ttaccagtgt
     7561 cgctggaata tataaatgtg agtgataacc atttgttcgc acttcctgaa ttacctgcgt
     7621 cactagaata tattaatgta agtgacaatc acctgtctgt acttccgagg ttaccaatgt
     7681 cattggaatt acttgatgca gccagaaatg ctttggaagt aataccagat tttccagaaa
     7741 gagatgatca tattataaga atattctggc ttaatcagaa ccggatcacg gcaattccgg
     7801 aaagcatact tggcctcagt tctgatagcg ttgtcaatct tagagaaaat caactatctc
     7861 ccagaataat gcaaactttg ttacaacaaa ccgccca
//
LOCUS       DAJTQY010000142         7804 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.142, whole genome shotgun sequence.
ACCESSION   DAJTQY010000142 DAJTQY010000000
VERSION     DAJTQY010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7804
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.142"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            1..1331
                     /gene="yjeM"
                     /locus_tag="OO349_003614"
                     /pseudo
     CDS             1..1331
                     /gene="yjeM"
                     /locus_tag="OO349_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
     gene            complement(1482..4793)
                     /gene="mscM"
                     /locus_tag="OO349_003615"
     CDS             complement(1482..4793)
                     /gene="mscM"
                     /locus_tag="OO349_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HCR5868541.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNIRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMELVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWASDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIMALMMFDNLDDREFSGS
                     LGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVAILAS
                     AVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAEMLAQ
                     RARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLWSEIH
                     SAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLELAIL
                     QHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFGLQEI
                     FANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNKAFIT
                     EQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFLVDLQ
                     QGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNGKQTG
                     RTLTSAGKGRQAGSL"
     gene            complement(4815..5783)
                     /gene="psd"
                     /locus_tag="OO349_003616"
     CDS             complement(4815..5783)
                     /gene="psd"
                     /locus_tag="OO349_003616"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HCR5868542.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(5880..6932)
                     /gene="rsgA"
                     /locus_tag="OO349_003617"
     CDS             complement(5880..6932)
                     /gene="rsgA"
                     /locus_tag="OO349_003617"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HCR5868543.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            7027..7572
                     /gene="orn"
                     /locus_tag="OO349_003618"
     CDS             7027..7572
                     /gene="orn"
                     /locus_tag="OO349_003618"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HCR5868544.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEVYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
BASE COUNT         1766 a         2025 c         2112 g         1901 t
ORIGIN      
        1 ttaatgatgg ctgaaatggg agctgcttat cgcaaagaag aaggtggtat ctattcctgg
       61 atgaataata gtgtcggacc acgttttgcc ttcattggta cgtttatgtg gttttcctct
      121 tatatcatct ggatggtgag tacctccgcg aaagtttggg taccgttctc aacattcctc
      181 tatggtagtg acatgaccca gcactggcgt attgccggac tggagcctac gcaggtggtt
      241 ggtctgctgg cagtagcatg gatgattctg gtcaccgtcg ttgcttcaaa ggggattaat
      301 aaaattgccc gcattactgc ggtgggcggt attgcagtaa tgtgtctgaa tttagtattg
      361 ctgttagtaa gcattactat tttgttatta aatggtgggc atttcgcgca ggatattaat
      421 ttccttgcat caccgaaccc gggttatcag tccggtctgg caatgctatc gtttgtggta
      481 tttgctattt ttgcctatgg cggaattgaa gcggttggtg gtctggtcga taaaacggaa
      541 aatccagaaa agaactttgc caaaggtatt gtttttgccg ctattgttat ttcaatcggt
      601 tattcgctgg caatattttt atggggcgtc agcacaaact ggcagcaggt attaagtaat
      661 ggttccgtta accttggcaa tattacctat gtgctgatga agagcctcgg ggtgacgctg
      721 ggtaacgcac tgcatttgtc acctgaagcg tcattgtcgc tgggcgtatg gtttgcgcgt
      781 attaccggac tttcgatgtt cctcgcttat accggtgcgt ttgctattca ccgctgaaag
      841 ccatcatcca ggggacgccg aaagcgttgt ggccggaacc gatgacgcgc ctgaatgcga
      901 tggggatgcc gtctatcgcc atgtggatgc agtgcgggtt ggttactgtc ttcattctgc
      961 tggtttcgtt tggtggcggt accgcatcgg cgttctttaa caagctgacg ctgatggcga
     1021 acgtgtctat gacgcttcct tacctgttcc tcgcgctggc tttcccgttt tttaaagcac
     1081 gtcaggatct cgacagacca tttgtgattt tcaaaacgcg tatgtcggca atgattgcga
     1141 cggtggttgt cgttctggtg gtgacatttg cgaacgtctt caccattatt cagcctgtgg
     1201 ttgaagccgg agactgggac agcacattgt ggatgattgg cggccctgtc ttcttctcgc
     1261 tgttagcgat ggcgatttac cagaactatt gcagtcgcat ggcgaataaa cctgagttag
     1321 ctctcgactg atcatatgaa atcctgcaac tcatcgtctc tccggcgatg agttgtcagg
     1381 aagtaatgcg tatggatgat actctccagg gccggtgata actgaagatg atgttaagct
     1441 gctaaatgag ccacgttgac atcatttggc agcttcatgc attacaaact tcccgcctga
     1501 cgacctttgc ccgcagacgt cagcgttctc cccgtttgtt taccgttgag gctttccaga
     1561 cgcatctgga agggcgggaa tggcatatcg ataccgtggg cgtggaagcc tgccagaatc
     1621 agctggtgga tctcatggcg tagcggcata cggtgaccca tctcagcggc gtaaatacgc
     1681 agctcgaaaa tctgaatccc ctgttgcaga tccaccagga agacttccgg tgccgggttg
     1741 tcgatcacca gcgagcagcg acgcgctgcg gtgagcagga tttccgtcac ttcttcactg
     1801 ttggcatcgg caggggccgg aatcgtcaac actacgcgcg tgaccgagtc agagagcgac
     1861 cagttgataa actgctcggt aataaacgcc ttgttcggca cgattatctc tttacggtcc
     1921 cagtcgctga tggtggtggc gcgggtgtta atttttgtca cgctaccagt aagatcgcgg
     1981 atcgttaccg tatcgccaat gcgaatcggt ttttcgaaca ggatgatcag gccagagata
     2041 aagttggcga aaatttcctg caaaccaaaa ccgagaccaa caccgagcgc ggcaaccagc
     2101 cactgcaatt tcgaccactc aataccaatc attgagaagc cgaccagccc gccaatcagc
     2161 atcagcagat attttgtgat ggtggtgatg gcgtaacccg tacccggcgt taaatccagg
     2221 tgctgcaaaa tcgccagttc cagcagcgcg ggcaggttgc gcaccagctg cgtggtgatg
     2281 ataaatacca gaatggcaat cagcaccgca ccgagggtaa ttggctccag actttctacg
     2341 ccctgtaccg tggaggtgac atcccacagc gaaatatttt cgaggaagcc gaaagcggaa
     2401 tggatttctg accacagcac aatgaccgaa agcagggcga tcaacatcaa aattgagcgc
     2461 accagccgca aggattgcgc actgatggca tcgagatcga cttcgctttc atcgacttca
     2521 attgctcctt ccgggctact gtgatgatgc gcttcctctt cgccacgcgc acgttgcgct
     2581 aacatctctg cccggcgatg cttcgcccgg tcaaacgcca gcctgcgacg ctggatcagc
     2641 atccagcggc ggataacgtg ataaaccacc agtagcagga accagatggc aaccgaggtt
     2701 tcaagcctcg ctaacagtgc ctgcgccgtt gccagataac ccaccgccga cgccagaatg
     2761 gcaaccagtg gtgcgccaat catcatgttc cacagcatat ggttggtaat gttgtcgccg
     2821 ctgccttctt tgttgagata caacggaata ccggcttttt tcaggcttag ggtgaccacc
     2881 gccagcgcac cgcaaatgag gataaagcaa agccgtccca gcgaaccgga aaattcgcgg
     2941 tcgtcgaggt tatcgaacat catcagcgcc ataatcagcc cgatgctcat caggtagtag
     3001 cgcatcccac gggaaacacg ttcgcgcggc cagccaaaat gagcgataaa caagccgttc
     3061 gggcgggcaa aggtggcgca aatcatcact acccacagca gcggcacggt ggccgttaca
     3121 ccatcgccaa tcgcgaccgc cagcggataa ggccacgcct cgcgcaagcc gtaacccagc
     3181 gtcatccaca gcaccggtaa cggtgacgcg acgagaatcg accagaaaag agtgcgcaac
     3241 gtcagccaga agtgatcctg agtcactttg ccgactttcg ccgccgaacg ttcaagaaaa
     3301 cgggtgaaat agcggcgcga gtaaatactg caaccgacca gaatcaacgc gccaaacagc
     3361 ggcaaaattg tctctttgct ggtcagcatc atcacactgg ctttgcccaa ctgactgaag
     3421 gtgtccagcg aaatgagacg acgcagatcc tgggcgattt ccagcggcca ggcgatggtc
     3481 atcgggcgca cgtcagaggc ccagaacaga tagcggtgcg ttgcttcgtt cacctctttc
     3541 agcgcatcct ccagttgccc gttggagact ttcagcttgg tcagttccaa tagtagcgtg
     3601 tcgccaccct gcaacaatga gttcagcaac tcacgctgag tgcgcagttg tgcttccaga
     3661 atacggtttt gctcggcggt cagcggctga ccgtcggcct gatggatttg ccgtagcagc
     3721 ggctgtttat taagtaaatc ctcataacgt aaccgttgca cacgcaactg cgccatttcg
     3781 gtatcaagct gttgtggttt tggcatttct ggtagccgtg ccacctgcgc ccgcagcgct
     3841 tcgccgagca ggttggacga tcccagccat tgcgactgtt cacgcagcgt attcaacgcc
     3901 tgccggacct gtaacgtctg gctggcagcc tgacgctgtt gcgaggcaac gagctccatc
     3961 cgctgcgcct gttgattcag cgctgccgat agttcgcggt taattttgaa ttgcgcgacg
     4021 atatctttcg gcaaatcggc actgttttct gccagtaatt cggtactttc cagcgcccgc
     4081 tccgcctcaa gctgacgttg gctattcaac tgattacgta aggcctgcaa atacgcatcc
     4141 agttgctggc tctctttttc cgccagctct gagcgtaagc gcgctaattc ctggcggtta
     4201 ttggcagaca gctgcgccag ctccagttca tcaacgagcg ccttaagacg tgcagagtca
     4261 gactgcaacg cgaaattttg tgcctgattg agcggagtat tgccggtaag cgttcccagg
     4321 cggcgctcga tctcatttaa ctgacggcgg gcgtcggttt gctgttgcgg cagttgattc
     4381 agcgaatcgg caatctcgcg ggctcgctcc tgctcttgct gggcctgacg gcttttatcc
     4441 agcaactggc tgctgacctg gagaatttcc tgattcagcg cgtcggtaga cattcccggc
     4501 gacacgctgc gcggctcgtc acgaatgttg tttaattgtg cgcgcagagt agcggagagt
     4561 ttcggataat tatcgataac ttgctgatat tgtttgatgc gctcaaggga accttttcgt
     4621 tcctcaagcg catttaaggc agactggagc gcctctacga cttccggctg tgcgggtttc
     4681 gccgcttttg cctgctccag ttcctgagtg atttgtttgc tatcgggggc cgtcgcggcg
     4741 tacgcccccc aactgaggca ccaggccatc agaaaagtga taatcaggcg cacgtcagcg
     4801 tttcctttga tggattagac ctggtctttt ttatcgtcaa ccaatgggct ggcgtcgtgt
     4861 tctgcttcga tctcttcagc aggaagtggg gcgggttcag cgtctggcgt aacaaaggtt
     4921 tcggtagata ctgccagcgg ctggccaatt ttcgtgacag acaggctttc cagttgctca
     4981 accagattca ctttacccgg tgcaaacagg ttgataacgg tggaaccgag tttaaagcga
     5041 cccatttcct ggcctttcag cagtgccaca gaaccgtcgt tttccccggc aggccaggtc
     5101 cagcgcttga tgataccttc gcgcggcggc gtaatggtgc ccgcccagac cgtctcaatg
     5161 ctgccaacaa tcgtcgctcc gaccagaatc tgcgccattg ggccaaattc ggtatcgaaa
     5221 aggcaaatca cgcgttcgtt acgggcaaac agattcggca cgttctgagc ggtgagatgg
     5281 ttaacggaaa agagatcgcc cggcacgtag atcatctcac gcagaatacc gttgcacggc
     5341 atgtgtacgc gatggtagtc acgcggggag aggtaagtgg tcacaaacgt accgttgcgg
     5401 aacaggtccg ccatcagata gttgcctgcc agcagggctt cgaggctgta gttgtggcct
     5461 ttggcttgca ggattttatc ttcttcgatt ttacccaact ggctgataac gccatcggca
     5521 ggcatgacca gtacattcgg atcggtatcg attgggcgta cttcgtcacg cagcggacgg
     5581 acaaagaatt cgttaaaggt gcggtagctg gcggtgtccg gcttttgcgc ctctttcatg
     5641 tcgaccttgt agtatttaac gaacagatcg ataaccagtt ttgtcagcca tcctgcccgc
     5701 ttgcttgcgc cccaacccgc caggcgagta agccatagtt tcggcagaat gtactgtagc
     5761 gaaagtttaa atgaatttaa caaggtagcc tccaggccat tgttttgtcg ttcctgatcc
     5821 ggccaacatg ccggatcctg aaaaaaaggg gacgattcta acgacggtta gcttaattgt
     5881 cagtcatccg tatcagaaaa gtttttacgc gtttttacct gcgccatgct ttccagaata
     5941 cggtgatagt tttcgaaacg ggtttccgcg attttccctt cctcaaccgc ttcccggata
     6001 gcgcagcccg gatcggtatc gtgtttgcaa tcgcgatatt tacacagacc taaatagtca
     6061 tggaattcga caaagccctg agtgatttgt tccggctcca ggtgccagag gccgaactca
     6121 cgcacaccag gggaatcaat cacatcaccg ccgtgcggga agtgatacag ccgagcggcg
     6181 gtcgtggtgt gctgaccgag ccccgagttg tcagagacat cgttggtcag gatctctttt
     6241 tgcagcccca gcaacgcgtt cagcaggctg gatttaccga cgccagactg cccggcaaaa
     6301 atgctgatgc gcccggtcaa cgcctcttcc agcggtttca gcccatcctg agtatggctg
     6361 gaaaccatca atacgcgata accgatgttg cggtagatat ccatctgctc gttgacgaac
     6421 gccatgcctt cgtcgtccag caggtctatc ttgttgagca cgataatcgg ctcaatctgc
     6481 aaggtttcgc aggccaccag gtaacggtcg ataatattga gcgacagctc cggcaaaatg
     6541 gcggagacaa tgacaatctg gtcgatgttg gcggctatgg gtttcacgcc gtcgtagaaa
     6601 tccggacgcg ttaacaccga ggtgcgctca tgcaccgctt ccacgatccc tttgacattt
     6661 acgccttccg ccgccggttt acccggacgc cagactacgc ggtcgccggt taccagcgaa
     6721 cggatggtac ggcgaatatt gcagcggtga acgtcgccat cggcggattc cacatcagcg
     6781 tgcataccaa agcggctgat gacgatacct tcatcaggct cgccaaacag attgtcgtcg
     6841 tagtcgggct tctccttaga cgttttaaga cgacgctggt gattggcgtt cacgcggcgc
     6901 tgctggcctt tggagagttt atttttactc aatcgtactg actcctggtc gcccctgatg
     6961 ggcaaaacat ctatgataca cgcaattgtg gatcaatata gtcactgtga atgggtggaa
     7021 aatagcatga gtgccaatga aaacaacctg atttggatcg atcttgagat gaccggtctg
     7081 gatcccgagc gcgatcgcat tattgagatt gccacgctgg tgaccgatgc caatctgaat
     7141 attctggcag aagggccgac cattgcagta catcagtccg atgaacagct ggcgctgatg
     7201 gatgactgga acgtgcgcac ccataccgcc agcgggctgg tagagcgcgt gaaagcgagc
     7261 acgatgggcg atcgggaagc tgaactggca acgctcgaat tcttaaaaca gtgggtgcct
     7321 gcgggaaaat cgccgatttg cggtaacagc atcggacagg accgtcgttt cctgtttaaa
     7381 tacatgccgg agctggaagt ctacttccac taccgttatc tcgatgtcag caccctgaaa
     7441 gagctggcgc gccgctggaa gccggaaatt ctggatggtt ttaccaagca ggggacgcat
     7501 caggcgatgg atgatatccg tgaatcggtg gcggagctgg cttactaccg cgagcatttt
     7561 atcaagctgt aaaatttttc ctggtcacgt aagcgccgga atccacttgc ggcggcgtga
     7621 ttttgacgct aaatcgaacg tcttgctgat aatttcagca cttgagataa aaacgcaaaa
     7681 aaaacttttt tggggggttg cagagggaaa gatttctcgt ataatgcgcc tcccgtaacg
     7741 acgcagaaat gcgaaaatta cgaaagcaaa attaagtagt acgcgggaat agctcagttg
     7801 gtag
//
LOCUS       DAJTQY010000143         7781 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.143, whole genome shotgun sequence.
ACCESSION   DAJTQY010000143 DAJTQY010000000
VERSION     DAJTQY010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7781
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.143"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            55..588
                     /gene="ymdB"
                     /locus_tag="OO349_003619"
     CDS             55..588
                     /gene="ymdB"
                     /locus_tag="OO349_003619"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HCR5868545.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYSYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            530..2011
                     /gene="clsC"
                     /locus_tag="OO349_003620"
     CDS             530..2011
                     /gene="clsC"
                     /locus_tag="OO349_003620"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HCR5868546.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEVILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(2019..3176)
                     /gene="mdoC"
                     /locus_tag="OO349_003621"
     CDS             complement(2019..3176)
                     /gene="mdoC"
                     /locus_tag="OO349_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HCR5868547.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            3570..5105
                     /gene="mdoG"
                     /locus_tag="OO349_003622"
     CDS             3570..5105
                     /gene="mdoG"
                     /locus_tag="OO349_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HCR5868548.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPRGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKITEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            5098..7641
                     /gene="mdoH"
                     /locus_tag="OO349_003623"
     CDS             5098..7641
                     /gene="mdoH"
                     /locus_tag="OO349_003623"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HCR5868549.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
BASE COUNT         1926 a         1863 c         2058 g         1934 t
ORIGIN      
        1 ttaaccaaaa agcctgctgt acacttaaga aacaagaagg tgtaaggagg cgttatgaaa
       61 acgcgtattc atgttgtgca gggtgatatt accaaactgg ccgttgatgt gattgtgaat
      121 gcggctaatc cgtcattaat gggaggcggc ggcgtcgatg gggccattca tcgcgcagcg
      181 ggtccggccc tgctggatgc ctgtttaaaa gtcaggcaac agcagggcga ttgccctacg
      241 gggcatgccg ttattacgct tgcaggcgat cttcccgcta aagccgtagt acacaccgtc
      301 gggccagtct ggcgtggtgg ggaacaaaac gaagaccagc ttttgcagga tgcctatctg
      361 aatagcctac gactggtggc ggcaaacagc tatacgtcag tggcttttcc tgcaatcagt
      421 actggggttt atagttaccc tcgtgcggca gcggctgaaa tcgcagttaa aaccgtttca
      481 gaatttatta cccgtcacgc tttacccgaa caggtatact ttgtctgtta tgatgaagaa
      541 aacgcccacc tctacgaaag actccttacc caacaaggag atgaatgatt tgccccggct
      601 ggcgagcgcg gtgctgccac tgtgttcgca acatcccggt cagtgtggtc ttttccctct
      661 ggagaaaagt ctggatgcgt ttgccgcccg gtatcgtctg gccgaaatgg cagagcatac
      721 gctggatgtt cagtattaca tctggcagga cgatatgtcg gggcggttac tgttttccgc
      781 cctgttagcc gcagcaaagc gtggcgttcg cgtccgtttg ttgctggacg acaacaatac
      841 gcccggactt gacgacattt tgcgcttgct tgacagtcat ccacgcattg aagtccggct
      901 ttttaatcct ttctcgtttc gcttgctacg tccgcttgga tatatcaccg acttttcccg
      961 tctcaatcgc cggatgcaca ataaaagttt cactgtcgat ggcgtggtta ccctagtggg
     1021 aggacgaaat attggtgatg cctattttgg tgcaggggaa gagccacttt tttcggattt
     1081 agatgtcatg gcaataggcc ccgtggtaga ggatgttgcc gatgatttcg cccgctactg
     1141 gtattgcaaa tcggtttcac ccttacagca ggtgctggat gtcccggaag gtgaaatggc
     1201 ggatcgcatc gagttacccg cctcctggca taacgatgcc atgacgcatc gttatttacg
     1261 caaaatggaa tccagtccat ttataaatca tctggttgat ggaacgttgc cgcttatctg
     1321 ggcgaagaca cgtttattaa gtgatgatcc ggcgaaaggg gagggcaagg caaaacggca
     1381 ttcactgtta ccgcagcgcc tgttcgatat catgggctca cccagtgaac gcatcgatat
     1441 tatctcttcc tattttgtac cgacacgcgc aggtgtggcg caactcttac ggatggtgag
     1501 aaaaggggta aagattgcga tcctaaccaa ttctcttgcc gctaacgatg ttgctgtcgt
     1561 ccatgccgga tacgcgcgct ggcgcaaaaa attgctccgc tatggcgtgg aattatatga
     1621 actcaagccg acgcgtgaac aaagcagtac gttacacgat cgcggcataa ccggtaattc
     1681 cggtgccagc ctgcatgcta aaacttttag catcgatggt aaaacggtgt ttatcggctc
     1741 tttcaatttc gatccgcgtt caacattgct caatactgaa atgggcttcg tgatagagag
     1801 cgaaacgctg gcacagttaa ttgataaacg ctttattcag agccagtacg atgcggcctg
     1861 gcagctccgt ctggacaggt ggggacggat caactgggtt gatcgtcatg caaagaaaga
     1921 ggttattctc aaaaaagaac ccgccaccag tttctggaag cgggttatgg tcagactggc
     1981 gtcgatattg cccgtggaat ggttattgta aatgtggctt aacgggctgg tgctttatcg
     2041 ttctcacgct taacaaccgg tttaccagag aacaaaaact tcagtaacgg gatgcgtaaa
     2101 tgaatttcat acagaattat cgcaatccct actacgaata tcaggccaca gagaaaacca
     2161 agccagttgg aggtgatgtg cggtgtaatg tatgcgccga aaaacagcgt taacgggtgg
     2221 tgaaccagat agataaacag cgatgcgtta acaaaataag tcacccgcgc tgactggaag
     2281 ttaagcaaac ggtggccaaa ggagaagacc acattcacca tccacaggcc gaggaccatg
     2341 gtgatcaccg actcggtttc gtacatccag gcatcgccac tgccatagcg ctggttgagt
     2401 aaataagcga caaacgccaa tgctgcagcg agggtacagc cacgagacgg cgtggtaaac
     2461 aaggctttaa gatgagggaa aatgaaagcc agtgcgccga ggataaagaa cggtaaataa
     2521 aatagcgttt gcatgacaat aaaattgaac atgccattac tcagaatggg tggatacaca
     2581 ataaaaatcg ttcttcttat taccgcatag ccgatgccga ggcataaaaa aatcaccgat
     2641 agttttacca tcgagaattt tttattcgtt ttatcagaat tttctaaatt atttctgatg
     2701 cgcttaaata tccatacaca cagcgtcgtc atgactacta acaccagtaa aaaccacagg
     2761 tgtgatatta attcccaggc caacgtatta tatttgtcat acaatgatag cccaggccaa
     2821 ctttccgctt tccctttgac atattgcagc ataataaatt gcggtaatgt cagtaggggg
     2881 atggctgtta acatcgggat acctacacgt tcgacacgta ctttccacca ttttttcaag
     2941 ggatagcgta aaaaaagcat gtaggaaaag tatccggata taacgaaaaa tacctgcatg
     3001 cggaacgagt ggatgaagtc attaaaaagg gttagccaca atgacggttc ggcgctattc
     3061 acatgccatg tatggctcga atagattaaa gaaatatgaa aagggatccc taacaacatc
     3121 agccaggcgc ggatggagtc gaggaaatat tcacgttgcg cgggtactgg attcatatat
     3181 ggttaactaa tctcggattt ttcgtcttat ccctgtcggg ttatgccttt aggcttgttg
     3241 ccatagcgac accgacctga ccgcgccagg cgcaggcttc aaggttttta tgcatagcat
     3301 catcgctacc actaaccaga atggaagcgt ctgtaagacg gttgataaat aaatttgctg
     3361 gcaaacccta cacgaagtcg atgcttctgt ctttaggaga agcacggaaa gtgaaaacgg
     3421 ttgcaatcag gtgcttaatc catgagccag cgtgctgaac gataccggga ttcagttgtc
     3481 ggaatggctg gttatccatt aaaatagatc ggatcgatat aagcacacaa agggggaagt
     3541 gcttactaat tatgaaacat aaactacaaa tgatgaaaat gcgttggttg agtgctgcag
     3601 taatgttaac cctgtataca tcttcaagct gggctttcag tattgatgat gtcgcaaagc
     3661 aagctcaatc cttagccggg aaaggctatg aggcgcctaa aagcaacttg ccctccgttt
     3721 tccgcgacat gaaatacgcg gactatcagc agatccagtt taatcatgac aaagcgtact
     3781 ggaacaatct gaagacccca ttcaaactcg agttctacca tcagggtatg tacttcgata
     3841 ccccggtcaa aataaatgaa gtgactgcca ccgcagtcaa acgaatcaaa tacagcccgg
     3901 attatttcac tttcggcgat gttcagcatg acaaagacac ggtaaaagac cttggttttg
     3961 ccggttttaa agtgctttac ccgatcaaca gcaaagataa aaacgatgaa atcgtcagca
     4021 tgctcggggc cagctatttc cgcgtgattg gtgcaggtca ggtttatggc ctttctgccc
     4081 gcggcctagc aattgatacc gctttgccat cgggtgaaga atttccacgc ttcaaagagt
     4141 tctggatcga gcgtccaaaa ccgactgata aacgtttaac catttatgca ttgcttgact
     4201 cgccgcgtgc gacaggtgct tacaaattcg tggttatgcc agggcgtgac acggttgtgg
     4261 atgtgcagtc gaaaatctat ctgcgcgata aagtcggcaa actgggggtt gcaccgttaa
     4321 ccagtatgtt cctgtttggg ccgaaccaac cgtcgcctgc aaataactat cgtccggagt
     4381 tgcacgactc taacggtctg tctatccatg ctggtaatgg cgaatggatc tggcgtccgt
     4441 tgaataaccc gaaacattta gcggtcagca gcttctccat ggaaaacccg cgaggctttg
     4501 gtctgttgca acgaggtcgt gatttctccc gctttgaaga tctcgatgat cgttacgatc
     4561 tccgtccaag cgcatgggtg actccgaaag gggagtgggg caaaggcagc gttgagctgg
     4621 tggaaattcc aaccaacgat gaaaccaacg ataacatcgt cgcttactgg acgccggatc
     4681 agctgccgga gccgggtaaa gagatgaact ttaaatacac catcaccttc agccgtgatg
     4741 aagacaaact gcatgcgcca gataacgcat gggtgcaaca aacgcgtcgt tcaacggggg
     4801 atgtgaagca gtcgaacctg attcgccagc ctgacggtac tatcgccttt gtggtcgatt
     4861 ttaccggcgc agagatgaaa aaactgccag aggatacccc ggtcacagcg caaaccagca
     4921 ttggtgataa tggtgagata gttgaaagca cggtgcgcta taacccggtt accaaaggct
     4981 ggcgtctggt gatgcgtgtg aaagtgaaag atgccaagaa aatcactgaa atgcgtgctg
     5041 cgctggtgaa tgccgatcag acgttgagtg aaacctggag ctaccagtta cctgccaatg
     5101 aataagacaa ctgagtacat tgacgcaatg cccatcgccg caagcgagaa agcagcattg
     5161 ccgaagactg atatccgcgc cgttcatcag gcgctggatg ccgaacaccg cacctgggcg
     5221 cgggaggatg attccccgca aggctcggta aaggcgcgtc tggaacaagc ctggccagat
     5281 tcacttgctg atggacagtt aattaaagac gacgaagggc gcgatcagct taaggcgatg
     5341 ccagaagcaa aacgctcctc gatgtttccc gacccgtggc gtaccaaccc ggtaggccgt
     5401 ttctgggatc gcctgcgtgg acgcgatgtc acgccgcgct atctggctcg tttgaccaaa
     5461 gaagagcagg agagcgagca aaagtggcgt accgttggta ccatccgccg ttacattctg
     5521 ttgatcctga cgctcgcgca aactgttgtc gcgacctggt atatgaagac cattcttcct
     5581 tatcagggtt gggcgctgat taatcctatg gatatggttg gtcaggattt gtgggtttcc
     5641 tttatgcagc ttctgcctta tatgctgcaa accggtatcc tgatcctttt tgcggtactg
     5701 ttctgttggg tgtccgccgg attctggacg gcgttgatgg gcttcctgca actgcttatt
     5761 ggtcgcgata aatacagtat atctgcgtca acagttggcg atgaaccatt aaacccggag
     5821 catcgcacgg cgttgatcat gcctatctgt aacgaagacg tgaaccgtgt ttttgctggc
     5881 ttgcgtgcaa cgtgggaatc agtaaaagcc accgggaatg ccaaacactt tgatgtctac
     5941 attctgagtg acagttataa cccggatatc tgcgtcgcag agcaaaaagc ctggatggag
     6001 cttatcgctg aagtcggtgg cgaaggtcag attttctatc gccgccgccg tcgccgcgtg
     6061 aagcgtaaaa gcggtaatat cgatgacttc tgccgtcgct ggggcagcca gtacagctac
     6121 atggtggtgc tggatgctga ctcggtaatg accggtgatt gtttatgcgg cctggtgcgc
     6181 ttgatggaag ccaacccgaa cgccgggatc attcagtcgt cgccgaaagc gtccggtatg
     6241 gatacgctgt atgcgcgctg tcagcagttc gcgacccgcg tgtatgggcc actgtttaca
     6301 gccggtttgc acttctggca acttggcgag tcgcactact ggggacataa cgcgattatc
     6361 cgcgtgaaac catttatcga gcactgcgca ctggctccgc tgccgggcga aggttccttt
     6421 gccggttcaa tcctgtcaca tgacttcgta gaagcggcgt tgatgcgccg tgcaggttgg
     6481 ggggtctgga ttgcttacga tctcccgggt tcttatgaag aattgccgcc taacttgctt
     6541 gatgagctaa aacgtgaccg ccgctggtgc cacggtaacc tgatgaactt ccgtctgttc
     6601 ctggttaagg gtatgcaccc ggttcaccgt gcggtgttcc tgacgggcgt gatgtcttat
     6661 ctctccgctc cgctgtggtt tatgttcctc gcgctctcta ctgcattgca ggtagtgcat
     6721 gcgttgaccg aaccgcaata cttcctgcaa ccacggcagt tgttcccggt gtggccgcag
     6781 tggcgtcctg agctggcgat tgcacttttt gcttcgacca tggtgctgtt gttcctgccg
     6841 aagttattga gcattttgct tatctggtgc aaaggaacga aagaatacgg cggcttctgg
     6901 cgcgttacat tatcgttgct gctggaagtg ctgttttccg tgctgctggc tccggtacgc
     6961 atgctgttcc atacggtctt cgttgtcagc gcgttccttg gctgggaagt ggtgtggaat
     7021 tcaccgcagc gtgatgatga ctccacttca tggggcgaag cgttcaaacg ccacggctca
     7081 cagctgctgt tggggttagt gtgggctgtt gggatggcgt ggctggatct gcgtttcctg
     7141 ttctggctgg caccgattgt cttctcgttg atcctgtcac cgtttgtttc ggtgatttcc
     7201 agccgtgcca ccgttggtct gcgcaccaaa cgctggaaac tgttcctgat cccggaagag
     7261 tattcaccgc cgcaggtgct ggttgatacc gatcggttcc ttgagatgaa tcgccaacgc
     7321 tcccttgacg atggctttat gcacgcagtg tttaacccgt catttaacgc tctggcaacc
     7381 gcaatggcga cagcgcgtca ccgcgccagt aaggtgctgg aaatcgcccg tgaccgccac
     7441 gttgaacagg cgcttaacga gacgccagag aagctgaatc gcgatcgtcg cctggtgctg
     7501 ctaagcgatc cggtgacgat ggcccgtctg catttccgcg tctggaattc ccctgaaaga
     7561 tattcttcat gggtgagtta ttacgaaggg ataaagctca atccactggc attgcgtaaa
     7621 ccggatgcgg cttcgcaata aaaacgtagt tgcctgatgc gctacgctta tcaggcctac
     7681 atcgttcctg caatttattg attttgcaag attttgtagg tcggataagg cgttcacgcc
     7741 acatccggca aaaacaacga gccaataaaa ataccggcgt t
//
LOCUS       DAJTQY010000144         7744 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.144, whole genome shotgun sequence.
ACCESSION   DAJTQY010000144 DAJTQY010000000
VERSION     DAJTQY010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7744
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.144"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            <1..1014
                     /gene="ydfJ"
                     /locus_tag="OO349_003624"
     CDS             <1..1014
                     /gene="ydfJ"
                     /locus_tag="OO349_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
                     /protein_id="HCR5868550.1"
                     /translation="ILLVTLRIIQGLGAGAEISGAGTMLAEYAPKGKRGIISSFVAMG
                     TNCGTLSATAIWAFMFFILSKEELLAWGWRIPFLASVVVMVFAIWLRMNLKESPVFEK
                     VNDSNQPTAKPAPAGSMFQSKSFWLATGLRFGQAGNSGLIQTFLAGYLVQTLLFNKAI
                     PTDALMISSILGFMTIPFLGWLSDKIGRRIPYIIMNTSAIVLAWPMLSIIVDKSYAPS
                     TIMVALIVIHNCAVLGLFALENITMAEMFGCKNRFTRMAISKEIGGLIASGFGPILAG
                     IFCTMTESWYPIAIMIMAYSVIGLISALKMPEVKDRDLSALEDAAEDQPRVVRAAQPS
                     RSL"
     gene            1103..2563
                     /locus_tag="OO349_003625"
     CDS             1103..2563
                     /locus_tag="OO349_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCR5868551.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLEHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYGMMLQEQAP
                     TLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(2599..2802)
                     /gene="ydfZ"
                     /locus_tag="OO349_003626"
     CDS             complement(2599..2802)
                     /gene="ydfZ"
                     /locus_tag="OO349_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HCR5868552.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(2980..3666)
                     /gene="ydfH"
                     /locus_tag="OO349_003627"
     CDS             complement(2980..3666)
                     /gene="ydfH"
                     /locus_tag="OO349_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YdfH"
                     /protein_id="HCR5868553.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(3755..4501)
                     /gene="ydfG"
                     /locus_tag="OO349_003628"
     CDS             complement(3755..4501)
                     /gene="ydfG"
                     /locus_tag="OO349_003628"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HCR5868554.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELTDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWSNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            4638..6683
                     /gene="dcp"
                     /locus_tag="OO349_003629"
     CDS             4638..6683
                     /gene="dcp"
                     /locus_tag="OO349_003629"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HCR5868555.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELITRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQLRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLHRQWRGKAPQIMPMLQHRGLNI"
     gene            complement(6728..7246)
                     /locus_tag="OO349_003630"
     CDS             complement(6728..7246)
                     /locus_tag="OO349_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HCR5868556.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
     gene            7522..>7744
                     /gene="ydeI"
                     /locus_tag="OO349_003631"
     CDS             7522..>7744
                     /gene="ydeI"
                     /locus_tag="OO349_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HCR5868557.1"
                     /translation="MKFQSIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISHK"
BASE COUNT         1942 a         1812 c         1954 g         2036 t
ORIGIN      
        1 atcttgctgg tgacgctgcg tattattcag ggattgggtg caggtgcgga aatttccggt
       61 gccggtacga tgctggcgga atatgcgcca aaaggtaagc gcggaattat ctcctcattt
      121 gtggctatgg gaactaactg cggcacctta agcgcaacgg caatctgggc ctttatgttc
      181 ttcattctca gtaaagagga actgctggcg tggggatggc gtataccgtt cctagcgagc
      241 gttgtcgtga tggtctttgc tatctggttg cgtatgaatc tgaaagaaag cccggtcttt
      301 gagaaggtta acgacagcaa ccaaccgaca gcaaaacctg cacctgctgg tagcatgttc
      361 cagagcaaat ccttctggct ggcaacaggg ctgcgttttg gtcaggcggg taactccggt
      421 ttaattcaga ctttccttgc aggctattta gtacagacgt tattgtttaa caaagcaatt
      481 ccaacagatg cattgatgat cagttcgatt ctcggcttta tgaccattcc gttccttggt
      541 tggttatccg ataaaattgg tcgccggatc ccgtatatta ttatgaatac ctctgcgatt
      601 gtgctggcgt ggccaatgct ttctatcatc gtagataaaa gctatgcccc gagcaccatt
      661 atggttgcac tgattgtgat tcataactgt gcggtgctgg gattatttgc tctggaaaac
      721 attaccatgg cagaaatgtt cggctgtaaa aaccgcttca cccggatggc tatttcaaaa
      781 gaaattggtg gtcttatcgc ttccggtttt ggtcctatcc tggcgggtat tttctgcacc
      841 atgacggaat cctggtatcc gatcgccatt atgatcatgg catattcagt gattggttta
      901 atctctgcgc tgaaaatgcc agaggtgaaa gaccgtgatt taagtgcgct ggaagacgct
      961 gcggaagatc aaccgcgtgt tgtaagagct gcgcaacctt ccagaagtct gtaaaccctc
     1021 aatcccttct cttaccggag aggggatttt tattcatata acaaaacata tagcttgcca
     1081 tatttaaatt taaggaattg ttatgggaaa taatttgtta tcagcaaaag cgacactccc
     1141 tgtttatgat cgtaataacc tggccccaag aattgttcat ttaggctttg gtgcatttca
     1201 ccgtgcgcat cagggcgtgt atgccgatat tcttgctaca gaacatttca gtgactgggg
     1261 atattatgag gtcaacttaa tcggcggcga acagcaaatt gccgatttac aacagcaaga
     1321 taatctttat accgttgcgg aaatgtcggc cgatgcgtgg acggctcgcg tcgttggcgt
     1381 cgttaaaaaa gccttgcacg tacagatgga tggcttagaa accgtgttgg ctgcgatgtg
     1441 tgaaccgcaa atcgcgattg tctctctgac aatcaccgaa aaagggtatt tccactctcc
     1501 ggcgaccgga cagttaatgc tcgagcaccc gatggtcgct gccgacgtgc aaaatcccca
     1561 ccagccgaaa acagcaacag gggtgattgt tgaggcgctg gctcgccgta aagcggcagg
     1621 acttcccgca tttaccgtca tgtcatgtga caacatgcca gaaaacggtc atgttatgcg
     1681 tgacgttgtc acttcctacg cgcaagccgt tgatgtaaaa ctggcacaat ggatcgaaga
     1741 taacgtgact ttcccatcaa caatggtgga ccgtattgtg cccgcagtga cagaggatac
     1801 gctggcgaaa atcgaacaac ttaccggtgt gcgcgatcct gctggcgttg cctgtgaacc
     1861 tttccgccag tgggttatcg aagataactt tgtagccgga cgtccggaat gggaaaaagc
     1921 gggagccgaa ctggttagtg atgtgctgcc ttatgaagag atgaagttgc gcatgctcaa
     1981 cggcagtcat tcattcctgg catatctggg ttatcttgct ggatatcagc acattaatga
     2041 ctgtatggaa gatgaacatt atcgtcatgc ggcgtatggc atgatgttgc aggaacaagc
     2101 gccgacgctg aaagtgcagg gcgttgattt gcaagattac gctaaccgat taattgcacg
     2161 ctatagcaac ccggcgttac gtcatcgaac ctggcagatt gcgatggatg gcagccagaa
     2221 attgccacag cggatgttgg attctgttcg ctggcatctg gcgcatgaca gcaagttcga
     2281 tctgctggcg ctgggcgtcg cgggttggat gcgttatgtc ggtggtgttg atgaacaggg
     2341 aaatccgata gaaatcagtg acccactgtt acctgttatt cagaaggctg tacaaagtag
     2401 tgccgaaggg aaagcgcgcg tccagtcatt gctggcgatt aaggcaatct ttggtgatga
     2461 tttgccagac aatagcttgt ttactgcaaa agtgacggaa gcgtacttgt ctttattagc
     2521 gcatggcgcg aaagcgaccg tggcgaaata ttccgtgaag taaaaaaagc aatgccgcca
     2581 tcggcggcat tcacacactt agttcatgcc gagacgaatc aggtccagag gtgccagttt
     2641 ctcttcacaa ccttcaacaa ctacggtttt tccgcggcgg atttgctcag cggtcagacc
     2701 ttcagtatca atcgacagga tcttgccagt gtgaccggtg ccgctaacca taacacggct
     2761 gccagtggtg attgcgttac ggttacgatc gtaggtcgtc ataggtgttt tctcctttct
     2821 gatttacagt cgaagcccac gtaacgggcg caatataaat actcctgtcg agtgaaatat
     2881 tttttgtttt tgatcaagct cacatctttt ttgatgcatg catgagccgt aaggcggggg
     2941 gaaaagcctc tcatcccgga tgggatgaga gtaaatgaat tactcttcgc taaaccagtc
     3001 gctgttttcc tggcggatct ggcgcacgga ttcgctgatt tcctgcaaat gttgcgtcat
     3061 tgcacgttct accgcatcgc catcacgttt ttgcagggca gagaaaatat caagatgctg
     3121 gcgtaacagc atttctggtg gagaaacatg gtcgaaactc atatagcgca cgcgatcaac
     3181 ggtcgctttc aggttctcaa tggtatccca cgccagttga cagtcggcaa tctgcgtcag
     3241 gagttgatgg aagttgtcat caagttcaaa aaaatcatcc agttgcttgc gctcaatggc
     3301 aatgcgttgc tggtgaagat tttgttccag ttggtagcac tggctttcgg taatcatgct
     3361 cgccgcccga cgcgccaccg cgcactcaat ggcctgacgg ataaaactgc cgttgcgcac
     3421 ctgggccatg gaaattttgt tgacgtagct gccacgctgc ggacgaattt gaatcaggcc
     3481 gttttccgcc agtttaataa aggcttcacg aaccggctgg cgtgacacat tgaaacgaac
     3541 agaaacttct ttttccgaca acggtgtgcc tggtgcaatc aggcaatgga caatgtcgcg
     3601 acgaagaata cgataaatct gctgattgac aggctgtgtg ggattaagtt gcgtttcgac
     3661 ggtcattcgt tcttgcttgt gagtgagtta actgcatgag tctaccactt ttttgtgaca
     3721 agcaataacc cggcagttac gccgggtata aaaattactg acggtggaca ttcagtccgg
     3781 catagctttg ggtaaccggc atcatttcca gggtattgat attgacgtga gcaggcagcg
     3841 ttgacaccca ccagacggct tcgctgacat cttctggcgt caatgcaacg gtattttgat
     3901 aggttttttc cgctttaccg tcatcgcctt taaagcggac attggaaaac tcggtgccac
     3961 ccaccagacc cggttcgatg tcggtgacgc gcaccgccgt accatgcaga tccgtacgca
     4021 gattcaggct aaactgacga acaaacgctt tcgtcgcacc gtaaacgtta ccaccggcat
     4081 acggccagct gcctgccgtt gagccaatgt taataatatg accatgatta cgttcaacca
     4141 tacccggtaa gacggcgcgc gtcatatata ctaggccttt gttgttggta tcaatcatcg
     4201 tttcccagtc ttcaacgctg gctttatgcg caggctccat gcccaacgcc aggccggcat
     4261 tatttaccag gatatcaata ttgctccact cggcagggag cgatgccagc atctcttcaa
     4321 tagcggcgcg gttgcgaacg tccagttggg cgatatacag attatctccc agttcgtctg
     4381 ttaactcctg caaccgctcc tggcgacggc cagtggcgat aactttatgc ccttgttgaa
     4441 taaaacgacg agtaatgcat tcaccaaaac ctgccgttgc tccagttact aaaacgatca
     4501 tctcactgtt cctcaacgct tttgtgtgtc aataacatag cacgtgatgc ttaagagcgg
     4561 gtaatactgg tttgcaagat tgcactccgt tatcagtaag cctacgctat ggaacacctc
     4621 gcgttcagga gaagaaaatg acaacaatga atcctttcct tgtgcaaagc acactgccgt
     4681 atctggctcc tcattttgat caaattgcca atcatcacta tcgcccggca ttcgatgagg
     4741 gaatgcaaca aaagcgggca gaaattgctg ccatcgcgct taacccgcaa acgcctgatt
     4801 tcaacaatac tattctggca ctggaacaaa gcggagaatt aattacccgc gttaccagcg
     4861 tcttttttgc gatgactgcg gcgcatacca atgatgaatt acagcgtctt gatgaacagt
     4921 tttccgctga actggcggaa ctggctaatg atatctatct gaacggtgaa ttattcgcgc
     4981 gggtagatgc tgtctggcag ctccgtgaat ccctggggct tgatagtgaa tccatccgcc
     5041 tggtggaggt tattcatcaa cgttttgtcc ttgccggagc caaacttgca caagctgata
     5101 aagcaaaatt aaaagtactg aatacagaag ctgcgaccct gaccagccag tttaaccagc
     5161 ggttacttgc agcaaataaa tccggcggtc tggttgtgaa cgatatcgcg cagctggcag
     5221 gaatgagtga gcaagagatt gcgctggcgg cagaggcggc tcgcgagaaa ggtctggata
     5281 acaaatggct gattccgctg ctgaatacca cccaacaacc ggcgcttgcc gaaatgcgcg
     5341 atcgtgcgac gcgtgaaaaa ctgtttattg cgggctggac gcgagcggaa aaaaatgatg
     5401 ccaatgatac ccgcgctatc attcaacgtc tggtagagat tcgcgcacag caggcgaaac
     5461 tgcttggttt tcctcattat gccgcatgga aaatcgccga tcagatggca aaaacgccag
     5521 aagcagcact caactttatg cgggaaattg ttccagcggc gcgtcaacgt gcgagcgatg
     5581 aattagcctc catacaggcg gttatcgata agcagcaggg cgggtttagc gcgcagccgt
     5641 gggactgggc attttatgcg gaacaggtac ggcgggagaa atttgatctt gatgaggcgc
     5701 agctcaagcc atattttgaa ttaaacacgg tgttaaatga aggtgtattc tggaccgcga
     5761 atcagctctt cggtattaag tttgtcgaac gttttgatat tccggtctac catcctgacg
     5821 ttcgtgtgtg ggaaattttt gatcataatg gcgtgggact ggcgttattt tacggtgatt
     5881 tcttcgcccg tgattcaaaa agcggcggtg catggatggg caattttgtt gagcaatcaa
     5941 cgcttaatga aacgcatccg gtaatttata acgtctgcaa ttatcagaaa cccgctgccg
     6001 gtgagcctgc gttgttactc tgggatgatg taataacctt attccatgaa tttggtcata
     6061 cgctgcacgg cctttttgcc cgccagcgtt atgccacgct ttccggcacc aacacgccgc
     6121 gtgattttgt cgaatttccg tcgcaaatca acgaacactg ggcaacgcat ccgcaggtat
     6181 tcgctcgcta cgcccggcat tatcagagcg gggcagcaat gcctgacgaa ctgcaacaga
     6241 aaatgcgtaa tgccagcctg ttcaacaaag ggtatgagat gagcgaactg cttagcgccg
     6301 cacttctcga tatgcgctgg cattgcctgg aagaaaacga agcaatgcag gatgtcgatg
     6361 attttgaatt gcgggcgctg gtggcggaaa atatggatct tcctgctata ccgccacgct
     6421 atcgcagcag ttatttcgcc catatttttg gtggcggata tgctgcaggt tattacgctt
     6481 atctgtggac gcaaatgttg gccgatgatg gttaccagtg gtttgttgag cagggcggat
     6541 taacgcgtga aaatgggcag cgttttcgcg aggcgatcct ttccagaggt aacagcgaag
     6601 atctggaacg cctgcatcga caatggcgtg gtaaggctcc tcagattatg ccgatgctgc
     6661 aacatcgtgg cttgaacata taaacaccac caatggctgg cctggagata tcgccaggcc
     6721 agcctggtta tagcagtaat tgcagcagct gggcgagggc aaacctcacc gctaaagcta
     6781 ataccgtttc gcagtcgcca gcaaaatgca taaccgcagt gtagttctgg cctttaatat
     6841 gccacgcgaa ccagacggta cccgctggcg taccatcttc accgccctcc ggtccgccgt
     6901 agccggtaat ggcaatactg acatcagcat ccgcacgctc tatggcacct gttgccattt
     6961 ctgctgccac tttctcactc accgcagaat atcgttcaag agactgctgg cttacgctga
     7021 ggattttcat ctttgcctga tcggtgaaag taacaaagcc tgcaccgtaa aatttgggtg
     7081 tatcttcagc tgcacacagg gcgctagcca acttaccgcc ggtgcatgat tcggcggtag
     7141 ttaagcgtaa ttgatccgcg ataagtcttt gactcaacgc ggatgtcagg ttttcaatag
     7201 tgtcggtatc tgcgagttgt acaattttat ctttattaat gttcactgtt ttttgtccac
     7261 gctattatat gtaaaggtga tttgcttttt ctgtatgctc gtccaatata ataattttca
     7321 aacatataaa tccgattatt ctgaaaacat gtttaagact cttcaatctt cttaacttta
     7381 gtgagtcaaa ttctctgaat atttgacagc tagtgggctg attttaaatg ccttataaca
     7441 attggcagta ttcttatttg gcaattataa aaatagcaac cagacttaat aagtcaaacc
     7501 aaacgcaatt aaggagttag tatgaaattt cagtccattg tattagcaag ttttcttgtc
     7561 atgccttatg cgttggcaga tgaccagggc ggtttaaaac aggatgcagc gccaccgccg
     7621 cctcatgcaa tagaagatgg ctatcgcgga accgatgatg caaaaaaaat gaccgttgat
     7681 ttcgcaaaaa acatgcacga tggcgcttcg gtttcactac gcggtaattt gatttctcac
     7741 aaag
//
LOCUS       DAJTQY010000145         7554 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.145, whole genome shotgun sequence.
ACCESSION   DAJTQY010000145 DAJTQY010000000
VERSION     DAJTQY010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7554
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.145"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            1..1158
                     /gene="rbsA"
                     /locus_tag="OO349_003632"
                     /pseudo
     CDS             1..1158
                     /gene="rbsA"
                     /locus_tag="OO349_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
     gene            1163..2128
                     /gene="rbsC"
                     /locus_tag="OO349_003633"
     CDS             1163..2128
                     /gene="rbsC"
                     /locus_tag="OO349_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HCR5868558.1"
                     /translation="MITQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALTLGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            2153..3043
                     /gene="rbsB"
                     /locus_tag="OO349_003634"
                     /pseudo
     CDS             2153..3043
                     /gene="rbsB"
                     /locus_tag="OO349_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            3169..4098
                     /gene="rbsK"
                     /locus_tag="OO349_003635"
     CDS             3169..4098
                     /gene="rbsK"
                     /locus_tag="OO349_003635"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HCR5868559.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPSLVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            4102..5094
                     /gene="rbsR"
                     /locus_tag="OO349_003636"
     CDS             4102..5094
                     /gene="rbsR"
                     /locus_tag="OO349_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HCR5868560.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(5060..6487)
                     /gene="mdtD"
                     /locus_tag="OO349_003637"
     CDS             complement(5060..6487)
                     /gene="mdtD"
                     /locus_tag="OO349_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HCR5868561.1"
                     /translation="MSDKKKRSMSGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLAYALSNSLPQL
                     VVFRVIQGIGDAMMMPVARPALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRIYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSMPNRVPSESE"
     gene            complement(6510..7202)
                     /locus_tag="OO349_003638"
     CDS             complement(6510..7202)
                     /locus_tag="OO349_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709569.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HCR5868562.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWITEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         1922 a         1828 c         1987 g         1817 t
ORIGIN      
        1 aaccatgtat gtcgaagcgg ataaattgct ggctaaactt aacctgcgct ttaaaagcga
       61 caagttagtg ggcgatcttt ccatcggtga ccagcaaatg gttgaaatcg ccaaagtgct
      121 gagctttgag tcgaaagtca tcattatgga tgaaccgacc gatgcgctga ccgataccga
      181 aaccgaatcc ctgttccgcg tcatccgcga gctgaaatcg caaggccact gtattgtcta
      241 tatctcccac cgcatgaaag aaatcttcga gatttgcgat gacgttaccg tttttcgtga
      301 tgggcaattt attgctgagc gcgaagtggc atcactgacc gaagattcgc tgattgagat
      361 gatggtgggc cgcaagctgg aagatcaata tccacacctg gacaaagcgc caggagatat
      421 ccgcctgaaa gtcgataatc tctgcggacc tggcgttaac gatgtctctt ttactttacg
      481 caaaggcgaa attcttggcg tctctggttt gatgggcgca ggtcgtaccg aactgatgaa
      541 agtgctctac ggcgcactgc cgcgcaccag cggttacgtc accctggatg ggcatgaagt
      601 cgttacccgt tcaccgcagg atggcctggc aaacggcatt gtgtatatct ccgaagaccg
      661 taaacgtgac ggtttagtgc tgggcatgtc agtaaaagag aacatgtcgc tgacagcgct
      721 gcgctacttc agccgcgctg gcggcagttt gaagcatgcc gatgaacagc aggctgtgag
      781 tgatttcatt cgtctgttta atgtgaaaac gccgtcgatg gaacaggcaa ttggtctgct
      841 ttccggtggc aatcagcaaa aagtggcgat tgcccgcggt ctgatgacac ccccaaagta
      901 ttgatccttg atgagcctac ccgtggcgta gatgtcggcg cgaaaaaaga gatctatcaa
      961 ctgattaacc agttcaaagc cgatggcttg agtatcattc tggtgtcatc ggagatgcca
     1021 gaagtattag gcatgagcga tcgcatcatc gtcatgcatg aagggcatct cagcggggaa
     1081 tttactcgtg agcaggccac ccaggaagtg ttaatggctg tcgctgtggg caagcttaat
     1141 cgcgtgaatc aggagtaaaa aaatgataac ccagactgtc tctggtcgcc gttatttcac
     1201 gaaagcgtgg ctgatggagc agaaatcgct tatcgctctg ctggtgctga tcgcgattgt
     1261 ctcgacgtta agcccgaact ttttcaccat caataactta ttcaatattc tccagcaaac
     1321 ttcggtgaac gccattatgg cggtcgggat gacgctggtg atcctgacgt cgggcataga
     1381 cttgtcggta ggttctctgt tggcgctaac cggcgcagtt gctgcatcta tcgtcggcat
     1441 tgaagtcaat gcgctggtgg ctgtcgctgc tgctctcacg ttaggtgccg caattggtgc
     1501 ggtaaccggg gtgattgtag cgaaaggtcg cgtccaggcg tttatcgcta cgctggttat
     1561 gatgctttta ctgcgcggcg tgactatggt ttataccaac ggtagcccag tgaataccgg
     1621 ctttactgag aacgccgatc tgtttggctg gtttggtatt ggtcgtccgc tgggcgtacc
     1681 gacgccagtc tggatcatgg ggattgtctt cctcgcggcc tggtacatgc tgcatcacac
     1741 gcgtctgggg cgttacatct acgcgctggg cggcaacgaa gcggcaacgc gtctttctgg
     1801 tatcaacgtc aataaaatca aaatcatcgt ctattctctt tgtggtctgc tggcatcgct
     1861 ggccgggatc attgaagtgg cgcgtctctc ctctgcacaa cccacggcgg ggactggcta
     1921 tgagctggat gctattgctg cggtggttct gggcggtacg agtctggcgg gcggaaaagg
     1981 tcgcattgtt gggacgttga tcggcgcatt aattcttggc ttccttaata atggattgaa
     2041 tttgttaggt gtttcctcct attaccagat gatcgtcaaa gcggtggtga ttttgctggc
     2101 ggtgctggta gacaacaaaa agcagtaata acgactacag gacatcttga atatgaacat
     2161 gaaaaaactg gctaccctgg tttccgctgt tgcgctaagc gccaccgtca gtgcgaatgc
     2221 gatggcaaaa gacaccatcg cgctggtggt ctccacgctt aacaacccgt tctttgtatc
     2281 gctgaaagat ggcgcgcaga aagaggcgga taaacttggc tataacctgg tggtgctgga
     2341 ctcccagaac aacccggcga aagagctggc gaacgtgcag gacttaaccg ttcgcggcac
     2401 caaaattctg ctgattaacc cgaccgactc cgacgcagtg ggtaatgctg tgaagatggc
     2461 taactaggcg aacattccgg ttatcactct tgaccgccag gcaacgaaag gtgaagtggt
     2521 gagccacatt gcatctgata acgtactggg cggcaaaatc gccggtgatt acatcgcgaa
     2581 gaaagcgggt gaaggtgcca aagttatcga gctgcaaggc attgccggta catccgcagc
     2641 ccgtgaacgt ggcgaaggct tccagctggc cgttgctgct cacaagttta atgttcttgc
     2701 cagccagcca gcagattttg atcgcaccaa aggtttgaac gtaatgcaga acctgttgac
     2761 cgctcatccg gatgttcagg ctgtattcgc gcagaatgat gaaatggcgc tgggcgcgct
     2821 gcgcgcactg caaactgccg gtaaatcgga tgtgatggtc gtcggatttg acggtacacc
     2881 ggatggcgaa aaagcggtga atgatggcaa actagcagcg actatcgctc agctacccga
     2941 tcagattggc gcgaaaggcg ttgaaaccgc agataaagtg ctgaaaggcg agaaagttca
     3001 ggctaagtat ctggttgatc tgaaactggt tgttaagcag tagttttaat caggttgtat
     3061 gacctgatgg tgacataaat acgtcatcga cagatgaacg tgtaatataa agaaaagcag
     3121 ggcacgcgcc accctaacac ggtggcgcat tttatggaca tcccgaatat gcaaaacgca
     3181 ggcagcctcg ttgttcttgg cagcattaat gctgaccaca ttcttaatct tcaatctttt
     3241 cctactccag gcgaaaccgt aaccggtaac cactatcagg ttgcatttgg cggcaaaggc
     3301 gcgaatcagg ctgtggctgc tgggcgtagc ggtgcgaata tcgcgtttat tgcctgtact
     3361 ggcgatgaca gcattggcga gagcgttcgc cagcagctcg ccactgataa catcgatatt
     3421 actccggtca gcgtgatcaa aggcgaatca acaggtgtgg cgctgatttt tgttaatggc
     3481 gaaggtgaga atgtcatcgg tattcatgcc ggcgctaatg ctgctctttc cccatcgctg
     3541 gtggaagcgc aacgtgagcg tattgccaac gcgtcagcgt tattaatgca gctggaatca
     3601 ccactcgaaa gtgtgatggc agcggcgaaa atcgcccatc aaaataagac tatcgttgcg
     3661 cttaacccgg ctccggctcg cgaacttcct gacgaactgc tggcgctggt ggacattatt
     3721 acgccaaacg aaacggaagc agaaaagctc accggtattc gtgttgaaaa tgatgaagat
     3781 gcagcgaagg cggcgcaggt actgcatgaa aagggtatcc gtactgtact gattacttta
     3841 ggaagtcgtg gtgtatgggc tagcgtgaat ggtgaaggtc agcgcgttcc tggattccgg
     3901 gtgcaggctg tcgataccat tgctgccgga gataccttta acggcgcgtt aatcacggcg
     3961 ttgctggaag aaaaaccatt gccagaggcg attcggtttg cccatgctgc cgctgcgatt
     4021 gccgtaacac gtaaaggcgc acaaccttcc gtaccgtggc gtgaagagat cgacgcattt
     4081 ttagacaggc agaggtgacg cttggctaca atgaaagatg ttgcccgttt ggcgggcgtt
     4141 tctacctcaa cagtttctca cgttatcaat aaagatcgct tcgtcagtga agcgattacc
     4201 gccaaagttg aagcggcgat taaagaactc aattacgcgc catcagctct ggcgcgtagc
     4261 ctcaaactca atcaaacaca taccattggc atgttgatca ctgccagtac caatcctttc
     4321 tattcagaac tggtgcgtgg cgttgaacgc agctgcttcg aacgcggtta tagtctcgtc
     4381 ctttgcaata ccgaaggcga tgaacagcgg atgaatcgca atctggaaac gctgatgcaa
     4441 aaacgcgttg atggcttact gttactgtgt accgaaacgc atcagccttc gcgtgaaatc
     4501 atgcaacgtt atccgacagt gcctaccgtg atgatggact gggctccgtt cgatggcgac
     4561 agcgatctta ttcaggataa ctcgttgctg ggcggagact tagcaacgca atatctgatc
     4621 gataaaggtc atacccgtat cgcctgtatt accggtccgc tggataaaac tccggcgcgc
     4681 ctgcggttgg aaggttatcg ggcggcgatg aaacgtgcgg gtctcaacat tcctgatggc
     4741 tatgaagtca ctggtgattt tgaatttaac ggcgggtttg acgccatgcg ccaactgtta
     4801 tcacatccgc tgcgtcctca ggccgtcttt accggaaatg acgctatggc tgttggcgtt
     4861 taccaggcgt tatatcaggc agagttacag gttccgcagg atatcgcggt gattggctat
     4921 gacgatatcg aactggcaag ctttatgacg ccaccattaa ccactatcca ccaaccgaaa
     4981 gatgaactgg gggagctggc gattgatgta ctcatccatc ggataaccca gccgaccctt
     5041 cagcaacaac gattacaact tactccgatt ctgatggaac gcggttcggc atagatttac
     5101 gctgtctttt gatcaaatta ttaccatcgg ttgttttcag aagcatgaac attgctgctg
     5161 aagcaacagt aataatgccc atcgtgataa acgtatagtg gaattgttcg acagtcgttg
     5221 tgccttccat cccttcataa atgcgaagga cggccgcact tacagcaacg cctaaactaa
     5281 tcgacagttg ctgcgtgacc gccagaacac tgttaccgct gctggcgttg tcatcggtca
     5341 gatcggcaag tgtgatggta ttcatcgcgg taaactgcgt cgacatagcc atccctaata
     5401 taaacaacgg caagatcagc atccatatcg ccattgccgg tgattgcaaa gagaactgag
     5461 cgatcattag cccaataatc accgtgatcc ccactaatgt atggcgatag cccagacgac
     5521 gtaagacttg ggtaaccatc gattttgcaa taatggaacc taacgctgtc ggtgccatca
     5581 tacagccggc aataaacgcc tgataaccaa atcctacctg taacatcaat ggcataagga
     5641 acggtacaca gccggtcccc agacgggttg caatattgcc tacgataccg atcgagaaag
     5701 tgcgggtttt aaataaatct aatgaaatta atgggtttgg cgtacgtcgc gcatggagaa
     5761 tatagagaag cagtaacccg atgctggtga caattaccgt caaggcaatc cagctggcga
     5821 caatcttttc cccgaatagc tctattccgc ttgaaaagag aacaaggctg aggccaaaca
     5881 gcaaaaagcc agtgatatcg aatctgcgtc gtgcggtggt gaaattgggc atatgtttgc
     5941 gcgcgtaaag aaggcccgca atacctatgg ggatattaat taaaaatatc cagtgccagg
     6001 ttgcccaggt gaccagcacg ccgccaagaa cggggcctaa aattggcccc accagacccg
     6061 gcatggcgac aaaattcaat accggaagaa gttcattacg aggataagca cgcagtaagg
     6121 ccggccgagc aacaggcatc atcattgcgt cgcctatccc ttgaataacc cggaagacaa
     6181 ccagctgtgg tagcgaatta gaaagtgcgt aggccagaga acccaacgtg aacagactca
     6241 cggcaagggt aaaaatgcga cgcgtaccga agcgatcggc tagccatccg cttaccggaa
     6301 taagcatcgc caccgtcagc gtataactga tgatggctga ttgcatcgcg agaggagaac
     6361 gattaaggct atgagcgatt gcgggtaagg cggtattcag aatagtggca tcaagtgcct
     6421 gcatgaagaa ggccatcgcc gcgatccacg gcaaacccga catactacgc ttctttttat
     6481 cgctcattca atgtcctgtt atcgggttat cacttatcag gtgagcgtag cagcgcctga
     6541 caagctttaa atgccgcgtc gccatcgctt tggataatcg catcgacaat cgcctggtgc
     6601 agatccagct ttatcactgt gtcgctggta attgacgtga agtaagtgtg ataaaccgaa
     6661 tggaatagcg aggcgaatga ggtcaaaaac ggattggcgc tcatttcata gatatgctca
     6721 tgccaggcca tatcgacttc gatccagcgt tcacggcgaa agttctcttt taatgccgcc
     6781 atttcggcca ttaacgtatt gagatgcgcc ttctgttccg cggtgccaac cgttgctgcc
     6841 agtaggcagg cttgcggctc cagacagata cgcataacca gaaagtgatc gatgacctga
     6901 tgaaagttct cttctgttat ccaccaagta agcaattcct gatcaagaaa attccagttc
     6961 gattgtggca tgacccgagt accaattcgc ggtcgcggta aaaccatccc ttttgccgtt
     7021 aacgttttga ccgcttcgcg taccgctgta cgactcactc caaattgctc gcccagctca
     7081 atctcaccgg gcaaaatggt gccgggttca tattcacctt ttaagatccg ttgcgccagc
     7141 ttctcagcca gaacatacga aaggtttttc tgtgcagcta actgttgtgc gcttaaaggc
     7201 attacttatc ttcctttttc tttttattcc tccttagtat gccaccagga agtgtgatta
     7261 cggttgcaaa aacggcaaat tgcttgtttt atggcacatt aacggggtat tttgctgaaa
     7321 aaatgcgcgg tcagaaaatt attttaaatt tcctcttgtc aggccggaat aactccctat
     7381 aatacgccac cactgacacg gaacaacggc aaatacgccg ccgggtcagc ggggttctcc
     7441 tgagaattcc gacagagaaa gcaaaaataa atgattgact ctgtagcggg aaagcgtatt
     7501 atgcacaccc ccgcgccgct gagaaaaagc gaagcgcact gctctttaac aatt
//
LOCUS       DAJTQY010000146         7364 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.146, whole genome shotgun sequence.
ACCESSION   DAJTQY010000146 DAJTQY010000000
VERSION     DAJTQY010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7364
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.146"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            185..838
                     /gene="nfsB"
                     /locus_tag="OO349_003639"
     CDS             185..838
                     /gene="nfsB"
                     /locus_tag="OO349_003639"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HCR5868563.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNKRKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNTTLTEV"
     gene            946..2193
                     /gene="ybdG"
                     /locus_tag="OO349_003640"
     CDS             946..2193
                     /gene="ybdG"
                     /locus_tag="OO349_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HCR5868564.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(2261..3637)
                     /gene="pheP"
                     /locus_tag="OO349_003641"
     CDS             complement(2261..3637)
                     /gene="pheP"
                     /locus_tag="OO349_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HCR5868565.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGYGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(3738..6881)
                     /gene="cusA"
                     /locus_tag="OO349_003642"
     CDS             complement(3738..6881)
                     /gene="cusA"
                     /locus_tag="OO349_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HCR5868566.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPKMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMPFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRN"
     gene            complement(6893..>7364)
                     /gene="cusB"
                     /locus_tag="OO349_003643"
     CDS             complement(6893..>7364)
                     /gene="cusB"
                     /locus_tag="OO349_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HCR5868567.1"
                     /translation="ESIAWLVKDASQFTLTVPARPDKTLTIRKWTLLPGVDAATRTLQ
                     LRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALIDTGNEQRVITVDADGRFVPK
                     RVAVFQASQGVTALRSGLAEGEKVVSGGLFLIDSEANISGALERMRSESATHAH"
BASE COUNT         1800 a         2083 c         1815 g         1666 t
ORIGIN      
        1 agcgttactt cgcgatctga tcaacgattc gtggaatctg gtggttgatg gtctggctaa
       61 acgcgatcaa aaaagagtgc gtccaggcta aagcgggaat ctatagcgca tttttctcgc
      121 ttaccatttc tcgttgaacc ttgtaatctg ctggcacgca aaattactta cacatggagt
      181 ctttatggat atcatttctg tcgccttaaa gcgtcattcc actaaggcat ttgatgccag
      241 caaaaaactt accccggaac aggccgagca gatcaaaacg ctactgcaat acagcccatc
      301 cagcaccaac tcccagccgt ggcattttat tgttgccagc acggaagaag gtaaagcgcg
      361 tgttgccaaa tccgctgccg gtaattatgt gttcaacaaa cgtaaaatac ttgatgcctc
      421 gcacgtcgtg gtgttctgtg caaaaaccgc gatggacgat gcctggctga agctggttgt
      481 tgaccaggaa gatgccgatg gccgctttgc cacgccggaa gcgaaagccg cgaacgataa
      541 aggtcgcaag ttcttcgccg atatgcaccg taaagatctg catgatgatg cagagtggat
      601 ggcaaaacag gtttatctca acgtcggtaa cttcctgctc ggcgtggcgg cgctgggtct
      661 ggacgcggtg cccatcgaag gttttgacgc cgcaatcctc gatgcagaat ttggtctgaa
      721 agagaaaggc tacaccagtc tggtggttgt tccggtaggt catcacagcg ttgaagattt
      781 taacgctacg ctgccgaaat ctcgtctgcc gcaaaacacc accttaaccg aagtgtaatt
      841 ctctcttgcc gggcatctgc ccggctattt cccctcagat tctcctgatt tgcataaccc
      901 tgtttcagcc gtcatcatag gctgctgttg tataaaggag acgttatgca ggatttaata
      961 tcccaggttg aagatttagc gggtattgag atcgatcaca ccacctcgat ggtgatgatt
     1021 ttcggtatta tttttctgac cgccgtcgtg gtgcatatta ttttgcattg ggtggtactg
     1081 cggaccttcg aaaaacgtgc catcgccagt tcacggcttt ggttgcaaat cattacccag
     1141 aataaactct tccaccgttt agcttttacc ctgcagggga ttatcgtcaa tattcaggcg
     1201 gtattctggc tgcaaaaagg caccgaagcg gcagatattc tgactacctg cgcgcagttg
     1261 tggatcatga tgtatgcgct gctttcagtc ttctcgttgc tggatgttat tttgaatctg
     1321 gcgcagaaat tcccggcggc atctcagtta ccgctgaaag ggatatttca ggggattaaa
     1381 ctgatcggcg caatcctggt cggcattttg atgatctcgc tgctgattgg tcagtcgcca
     1441 gcgattctga tcagcggtct tggtgcaatg gctgccgtgc tgatgttggt atttaaagat
     1501 ccgattcttg gtctggtggc aggtattcag ctttccgcga acgatatgct gaaactgggc
     1561 gactggctgg aaatgccgaa atacggcgcg gatggcgcgg tgatcgatat tgggttaacc
     1621 accgtcaaag tgcgtaactg ggacaatacc attaccacta ttcccacctg gtctctggtt
     1681 tctgactcct ttaaaaactg gagcgggatg tcagcatctg gcgggcgacg tattaagcgc
     1741 agtatcagta ttgatgtcac cagtattcgt tttcttgatg aagacgaaat gcaacgtctg
     1801 aataaagcgc atttgttaaa gccttattta accagccgcc atcaggaaat taatgagtgg
     1861 aatcgccagc aaggttctac ggagtcggta ttaaacctgc gccgaatgac caatattgga
     1921 acctttcgtg cctatctgaa cgaatatctg cgtaaccatc cgcggattcg taaagatatg
     1981 accttaatgg tacgccaact ggctccaggt gataacggtt taccgctcga gatctacgcg
     2041 tttactaaca ccgtggtgtg gctggaatat gaaagcattc aggctgatat attcgatcac
     2101 atatttgcga ttgtcgaaga gtttggtctg cgacttcatc agtcgccaac cggcaatgat
     2161 attcgctctc tggcgggtgc atttaagcag taattaaaaa aaccgctctc atcgaatgga
     2221 tgagagcgga catcgttttg tcggatgtag cgtgaatgcc ttatttccga cgcagcgttt
     2281 taaatgccac aaacaggaat acaatccaca ccggcagcag gatcgctgac aagcgcatat
     2341 catccatcgt gcacatcagc agcaaaatca tgccgaggaa ggcgatgcaa agatagttgc
     2401 cgaacggata gagcagcgcc ttaaactgtg tttcacgccc ctgacgtcgc atcgccgcgc
     2461 gaaaacgcag atgcgccagg cagatcataa tccagttcaa cagcaacgtt gctaccacca
     2521 gcgccatcag cagaccaaac gctttttgcg gcagcagata gttgattaac accaccagcg
     2581 aagtgatcgc tccggaaagc atcagcgagt taatcggcac accgcgacgg ctgacgcgag
     2641 tcaaaaactt cggcgcatta ccctgcacag aaaggccaaa cagcatgcgg ctgttagagt
     2701 aaaccccgct gttatacacc gacagcgatg ccactagaat gacgaagttc agcgcagaag
     2761 ctaccacatt gctgtcgaga ttatggaaaa tcatcacaaa cgggctactg ttggatttca
     2821 cttccaccca cggatagagt gccagtaaaa ccaccagtga accgatataa aacagcagga
     2881 tgcgatacac cacctgattt accgcttttg gaatgctttt ttccggatcg cgcgcttcag
     2941 cggctgtaat cccaatcagc tccagaccgc cgaaggagaa cataattacc gccagcgaca
     3001 aaatcagccc attccagccg gtggcgaaga aaccaccgta gcgccagagg ttgtcgatac
     3061 tggctttctc gccgccgtaa ccagaaaaca gcagccacag gccaaagccg atcataccga
     3121 tgatcgccag cactttaatc agcgcaaacc agaactcggt ttcgccatat aagcgcacgt
     3181 tcaccaggtt aacggcgttg atgataataa agaaggcggc agcccaaatc cacgttggaa
     3241 catccgggaa ccagtactgc atatagatgc ccgcagcggt cagctctgcc attcccacca
     3301 gcacgaacat tacccagtag ttccagccag agaggaagcc cgcaaacggt ccccagtatt
     3361 tataggcaaa gtgggcaaat gaaccggata ccggctcttc gaccaccatc tcgccaagct
     3421 ggcgcataat caggaaagcg atgatcccgg cgacgccgta gcccagcaat acagccggac
     3481 ccgccatctg aatcgccggg ccaatgccaa gaaacagacc agtaccaatt gcgccaccca
     3541 gcgcaatcag ttgaatatga cggttatgta atccgcgatg aagcgtcggc tcttgattcg
     3601 acgcagtatc ttccgatacg gttgacgcgt ttttcacgcc tttcccctgt gtgtcttttt
     3661 tgttgagggg caccttttaa catttagtgc ccatcgtcgc aagacacaat ccacacggtt
     3721 aaacgaggta tcctgcttta attccgtacc cgatgtcggt gcagccacat cagcttatac
     3781 gccgccggga taataaacag cgacagcaaa ggtgcggtga tcatgccgcc aatcatcggt
     3841 gcggcaatcc ggctcatcac ctctgaacca gcccccgttc cccacagaat cggcagcaga
     3901 ccggcgataa tcaccgccac cgtcatcgct ttcgggcgca cgcgcaggac cgcgccgtga
     3961 tacaacgcct catccagctt ctgctcgctg aatgtttgtg gattattcaa cgacggctcg
     4021 gcctctatgg cgtgacgtaa atacatcagc atcaccacgc caaattcggc ggcgaccccg
     4081 gcaagggcaa taaagccggt acccgtcgcc acagaaagat gaaagcccat ccaccacagt
     4141 aaccagatgc cgcccaccag cgcaaacggt acgctgctga taatcagcaa cgcttcgccc
     4201 acccgacgga atgccagata caacagcacg aagatgatca tcagtgtcat cggcaccatg
     4261 agtttcagct tatggttggc gcgctccagt agctcgaact gcccggagaa tgccacgctg
     4321 gtgcccggtt ttaactgcac tttttcagct atcgcttttt gcaaatcgtg aaccaccgac
     4381 accatgtcac gatcgcgggc atcgatataa atccagctcg tcgggcgcgc attctcggtt
     4441 ttcagcatcg acggtccggt agagacttta acgtcggcca cgtctgccag ggtgatttgc
     4501 tgcttcatcg gcgtcaggat cggcaactgg cgcagtgcct gcggactatc gcgccagctt
     4561 tgcggataac gcagattaat tggataacgg gcaatccctt ccaccgtttc gccaaccatc
     4621 gccccgccca ccgcagaagt cacaaacaac tgcacatccg ccaccgtcat accgtaacgt
     4681 gcggcttttt cacggttaat ctcaacgttg atatagcgcc caccttccag ccgctcagca
     4741 agcgcagaag ctacgcctgg caccgttcgc gccacttctt caatttgctc agccatcgcg
     4801 tcgatatccg ccagcacagt gccggaaact ttaatgccga tggggctttt aatgccggtt
     4861 gagagcatat cgatacggtt acgaattggc ggcacccaca gattcgccag ccccggcaga
     4921 cgcacggtgt tatccagttc ctcaatgatt ttgtccattg tcatgcctgg ccgccactga
     4981 tcctgtggct taagctggat ggtcgtttct accatctcca gcggagcaga atcggtggcg
     5041 gtttccgctt tcccggtttt gccaaatacc cgcgccactt caggtacgct cataattagc
     5101 ttgtcggttt tttgcagcat actcgccgcc tctgctgcgg aaatccccgg cagcgtcgat
     5161 ggcatataca acaagtcgcc ttcattgatc tgcggtaaaa attccccgcc aactttattg
     5221 agcggccaga gaaccgtcag caccgaaagc gccgccacca gcagcgtggt tttcggccag
     5281 tgcagtactt tcagcaacag cggatgataa acacgaatca aaaagcgatt gagcggatta
     5341 ctgctttccg gcggaatttt gccacggatc cagtaaccca tcaggatagg gatcactacg
     5401 atcgccagca gcgccgcacc cgccatcgca tacgttttgg tgaacgccaa cgggccaaac
     5461 agacgccctt cctgcccttc cagggtgaag atcgggataa acgacaacgt gataatcagc
     5521 agactgataa acagcgccgg ccccacttca acagacgcat cggtgatcac ctgccagcgc
     5581 gttttattat ccagcgtggc gtcaggatgc tggtgctgcc actcttccag ccgtttatgc
     5641 gcattctcga tcatgacgat agcggcatcg accatcgccc cgacggcaat cgcaatgcca
     5701 cccagcgaca taatattggc attcagcccc tggaagggca tgacaataaa agcaatacac
     5761 aaccccagcg gcaacgaaat aatcgccacc agcgccgagc gcacatgcca gagaaacagc
     5821 gcgcagacta ccgccaccac aataaactct tccagcaact tgccgctgag gttgtcgatg
     5881 gcccggtcaa tgagctggct gcggtcgtat gttgtaacta tctccacgcc ttccggcaga
     5941 ctacttttca gcgtttccag tttgtccttc acggcggcga tcacttctcg ggcgtttttg
     6001 ccggatcgca ggatcaccac cccgcccgcc acttcgcctt cgccgtttag ttcggcaatg
     6061 ccccggcgca tcttcgggcc aatctggacc ttcgcaacat cgcgcagata aacgggcacg
     6121 ccattttcac tggcttttaa aacgatgtga ttaaagtcgt cgagcgtttg tagatagccg
     6181 ctggcgcgca ccatatattc tgcttccgcc agttcgattg acgaaccgcc cgcttcctgg
     6241 tttgaagcat ccagcgcgct ttttacttcg gcgagactga tgccatactg cgccaggcgc
     6301 tgaggatcga taaccacctg atactctttc accacaccgc ctaccgacgc cacttccgca
     6361 acgtcaggaa tggttttcag ctcatatttg agaaaccagt cctgcaatga gcgtaaatcg
     6421 gcaagatcgt gcttaccgct gcgatccacc agtgcatatt catagatcca gccaacaccc
     6481 gtggcatctg gtcccagctc ggcgctgact cccgcaggca gcttaccctg tacctggttg
     6541 aggtactcca gcacccgcga acgcgcccag tacggatcgg tgccatcttc gaaaatgaca
     6601 tacacataag agtcgccaaa ctgcgagaaa ccacgcacag tcttcgcgcc aggcaccgac
     6661 aacatggtgg tggttagcgg ataagtcacc tgattttcaa cgatttgcgg tgcctggccg
     6721 ggatagctgg ttttaataat cacctgcaca tcggagagat ccggcaacgc atccactggc
     6781 gtattaatga tggtccaggt gccccagatg ctcagaaaca acgcgcccat cagcaccaaa
     6841 aaacggttcg ccaccgagcg acgaataatc cattcaatca ttggttattc cctcaatgcg
     6901 catgggtagc actttcagag cgcatccgct ccagtgcgcc agaaatattg gcttcggaat
     6961 caatcaggaa caggccgccg gaaaccacct tttcaccttc cgccagacca gagcgtaatg
     7021 cggtgacgcc ttgcgatgcc tggaaaacag caacgcgttt cggtacaaag cgcccgtcgg
     7081 catcaacggt aatcacccgc tgttcgttgc cggtatcaat cagcgcctgt gacggaatga
     7141 gcagcatcgg ttcgctggcg gtgttgagtt gcaaccaggc gttcatgccc ggttttagcg
     7201 cctcgtcggc gttgtcgact tccagacgca gctgcagcgt gcgggtcgcg gcatccacgc
     7261 caggtagcag cgtccatttg cggatggtga gtgttttatc cggtcgcgcc ggaacggtga
     7321 gggtaaactg cgaggcatct ttcaccagcc aggcgatgga ctcc
//
LOCUS       DAJTQY010000147         7015 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.147, whole genome shotgun sequence.
ACCESSION   DAJTQY010000147 DAJTQY010000000
VERSION     DAJTQY010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7015
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.147"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(<1..124)
                     /locus_tag="OO349_003644"
     CDS             complement(<1..124)
                     /locus_tag="OO349_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4102 domain-containing protein"
                     /protein_id="HCR5868568.1"
                     /translation="MAISDTKLRTIYGKPYSGPQEVADADGLSVRISPKGVIQFQ"
     gene            306..1730
                     /gene="sbcB"
                     /locus_tag="OO349_003645"
     CDS             306..1730
                     /gene="sbcB"
                     /locus_tag="OO349_003645"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HCR5868569.1"
                     /translation="MTDTDKQPTFLFHDYETFGTHPALDRPAQFAAIRTDDEFNVIGE
                     PEVFYCKPADDYLPRPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGYN
                     NVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGL
                     PSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMA
                     LIDVPQMKPLAHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMADLAGDISPLLELDID
                     TLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNLK
                     ILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPA
                     LDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRHQVFTPEFLQGYADELQMLV
                     QQYADDKEKVALLKALWQYAEEIV"
     gene            complement(1773..2000)
                     /locus_tag="OO349_003646"
     CDS             complement(1773..2000)
                     /locus_tag="OO349_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HCR5868570.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(2014..3072)
                     /gene="yeeE"
                     /locus_tag="OO349_003647"
     CDS             complement(2014..3072)
                     /gene="yeeE"
                     /locus_tag="OO349_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HCR5868571.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISAQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYLFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYTTEHNSIAETFNLSVWPLVTVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGVTSPTANILQFLVAGDVKYINWGVFLVLGIFVEAFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(3251..4609)
                     /gene="plaP"
                     /locus_tag="OO349_003648"
     CDS             complement(3251..4609)
                     /gene="plaP"
                     /locus_tag="OO349_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HCR5868572.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANVNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(4599..4661)
                     /gene="yoeI"
                     /locus_tag="OO349_003649"
     CDS             complement(4599..4661)
                     /gene="yoeI"
                     /locus_tag="OO349_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HCR5868573.1"
                     /translation="MGQFFAYATVITVKENNHVA"
     gene            complement(4822..5805)
                     /locus_tag="OO349_003650"
     CDS             complement(4822..5805)
                     /locus_tag="OO349_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5868574.1"
                     /translation="MKPLLDVLMILDAIEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAIQRKIIMTSLSFSCHFPPI"
     gene            complement(5851..6675)
                     /locus_tag="OO349_003651"
     CDS             complement(5851..6675)
                     /locus_tag="OO349_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5868575.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLQPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(6758..7012)
                     /gene="yoeB"
                     /locus_tag="OO349_003652"
     CDS             complement(6758..7012)
                     /gene="yoeB"
                     /locus_tag="OO349_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HCR5868576.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
BASE COUNT         1904 a         1831 c         1637 g         1643 t
ORIGIN      
        1 actggaactg gatgaccccc ttcggtgaaa ttcgtacgct gaggccatcg gcatcagcca
       61 cttcttgtgg gcccgaatat ggtttaccat aaatagtacg cagttttgtg tcgcttatag
      121 ccataaataa tatttggtac gctcagaaat aatagtttgg cacacatctg gtacacaata
      181 tcacatggac aacagcgcac agtaaacaac tatatttgac gttcgtagac atactcaggt
      241 gaaaaaaggg ttgcttttcg agttataaca gacagttaat taactacagt ggacaatgct
      301 agacaatgac agacacagat aaacaaccta ccttcctctt tcacgattac gaaacctttg
      361 gcacgcaccc cgcgttagat cgccctgcac agttcgcagc cattcgcacc gatgacgaat
      421 tcaatgtcat cggcgaaccc gaagtctttt actgcaagcc cgcggatgac tatttacccc
      481 ggcctggagc agtattaatt accggtatta ccccgcagga ggcacgggcg aaaggagaaa
      541 acgaagccgc gtttgccgcc cgtattcact cgctttttac cgtaccgaag acctgtattc
      601 tgggctacaa caatgtgcgt ttcgacgacg aagtcacacg caacgttttt tatcgtaatt
      661 tctacgatcc ttacgcctgg agctggcagc atgataactc gcgctgggat ttactggatg
      721 ttatgcgtgc ctgttatgcc ctgcgcccgg aaggaataaa ctggcctgaa aatgatgacg
      781 gtctaccgag ctttcgcctt gagcatttaa ccaaagcgaa tggtattgaa catagcaacg
      841 cccacgatgc gatggctgat gtgtacgcca ctattgcgat ggcgaaactg gtaaaaacgc
      901 gtcagccacg cctgtttgat tatctcttta cccatcgtaa taaacacaaa ctgatggcgt
      961 tgattgatgt tccgcagatg aaacccctgg ctcacgtttc cggaatgttt ggggcatggc
     1021 gtggcaatac cagctgggtg gcaccgctgg catggcatcc tgaaaatcgc aatgccgtaa
     1081 ttatggcgga tttggcagga gacatttcgc cattactgga gctggatatc gacacattgc
     1141 gcgagcgttt atataccgca aaagccgatc ttggcgataa cgccgccgtt ccggttaagc
     1201 tggtgcatat caataaatgt ccggtactgg cccaggcgaa tacgctacgc ccggaagatg
     1261 ccgaccgact gggaattaat cgtcagcatt gcctcgataa cctaaaaatt ctgcgtgaaa
     1321 atccgcaagt gcgtgaaaaa gtggtagcga tattcgcgga agccgaaccg tttacacctt
     1381 cagataatgt ggatgcacag ctttataatg gctttttcag tgacgccgat cgtgcagcaa
     1441 tgaaaattgt gctggaaacc gagccgcgta atttaccggc actggatatc acttttgttg
     1501 ataaacgaat tgaaaagttg ttgttcaatt atcgggcacg caacttcccg gggacgctgg
     1561 attatgccga gcagcaacgc tggctggagc accgccacca ggtcttcacg ccagagtttt
     1621 tgcagggtta tgctgatgaa ttgcagatgc tggtacaaca atatgccgat gacaaagaga
     1681 aagtggcgct gttaaaagca ctttggcagt acgcggaaga gatcgtctaa tttgaagccg
     1741 ccgccgctgg tacggcggag gacaatgttg agtcaggctt tttgaacggt gatgctccac
     1801 gctgcatcgc caatctgctg ataatcggtg atggcatgtc cttcttctgc cgcccactgt
     1861 gggattgctt cagttgcctg ggtgcaatca aactcaatta ccaactcatc gccgctggcc
     1921 atttctgcca gtgctgcttt tgcttctatg agtggaaacg gacagacctg cgtcaccaca
     1981 tccagctttt taatcgccat ctttacacct tacttaattt gccgcagcag ttgccagtcg
     2041 cgccttacgc tgcggtcgaa catacacaag ccaggacgca gtccagactc cgagaatcat
     2101 aaatacaagg ccaatccagc cctgccaggt catcattgca gtcataacca gtccattacc
     2161 gatagagcaa ccacctgcaa tactggcacc gaagcccatc agtacaccac ctaacccgct
     2221 acgtaatgtt gtttgtgcat cagctgcgcg aacgcggaac tcacggctcg ctttggctgc
     2281 aataaatgcc tccacgaaga tccctaacac caggaaaacg ccccagttaa tgtatttcac
     2341 gtcacccgcg accagaaatt gaagaatatt ggccgttggg gaagtgactc ctaacccaaa
     2401 catgcgtccg gttgcttcac tcaggggcca ggctaaaagc gcaatcaaac cgatgagtac
     2461 agcagtgaca aagggatgcc agcgtttttc aaacagaata tgagcgatcc cggttcggcg
     2521 cggcggtaag gtcgcgactt tgagttttgg cttcttcaac tctttcatca ccacccagag
     2581 cgttatcacc agcaaaacgg taaccaacgg ccacacagac aaattaaaag tctcagcaat
     2641 agagttatgt tcagtagtgt agtgctgcaa ggtttgattt aaaccactgg cgtgtggaga
     2701 acgcatcacc gcactcatca ccatataagt gaaaagcgcg atccaactgc cgatcaatcc
     2761 ctcacccgcg cgataccagg tcccggtggc acatccaccc gccagaacaa ttcccagccc
     2821 gaaaagatac ccacctataa cagtaccaag ccacgggaac gccccggctt cgtaagtcag
     2881 taggcccgcc tgaattaacg caaaaacccc cacgctttgt gccgaaattg caatcagcaa
     2941 ggcgtaaaac atgcgattat ttttcgcgat atacatatcg cgaaaaccac cagtcaggca
     3001 aaaacgccca cgctgcatga caaatcccag cagagcacca caaattagcc cgcttaatat
     3061 cattgaaaac ataaattaat taaccagatg aatgttaatg gggaaatttt tcatgactgg
     3121 tggaatgcag accaataacc aaattctcta aattagaaca aatggttatt aatgaggatt
     3181 tcgataaaaa agaataaaaa aaccggagcc aaatgttcat tcgactccgg tttattatta
     3241 gaaaagatga ttatgctacg tcttcgtact gcggaaccgg attacggaag ctcttggtga
     3301 cgcaagccag gtaaatcaga ccgatagcag cccagatcag acccagaacc attgagcttt
     3361 cttccaggtt aacccacagc gcaccaacgg tcagcgcacc acacatcggc aggaacagat
     3421 actggaagtg atctttcagc gtcttgttac gcttctcacg gatccagaac tgcgagatga
     3481 ccgacaggtt aacaaaggtg aacgccacca gcgcaccaaa gttaatcagc gccgttgcca
     3541 ttaccaggtc gaagttgatt gccagcaggg caatcgcgcc aaccaggatg atgttcatcg
     3601 ccggagtacg ccatttcggg tgtacataac cgaagaagct tttcgggaac acgccgtcac
     3661 gacccattac gtacatcaga cgcgctacgc ctgcgtgcgc cgccataccg gacgccagta
     3721 cggtaatggt ggagaagatc agcgcgccaa cctggaacgc tttacccgca acgtacagca
     3781 taatttcagg ctgtgacgca tccggatctt taaagcgaga gatatccggg aagtacagct
     3841 gcaggaagta agttgcaaag atgaagatca tgccgccaat cagcgcggtc aggaaaatcg
     3901 cacgcgggat cacgcgctct gcatctttgg tttcttccga cagattgctg atgccgtcaa
     3961 agccggtaaa ggagaagcac aggattgtcg ccccggtaat catcgggata acatgcgcat
     4021 cgccagacca gaacggacgc gtgctcgcca gcgtaccagc gccttcacct tcaaatacgc
     4081 cataaacaac catgcccaga atcaccgcga tcagcactac ctgcaacacg acgattacag
     4141 tgttgacgtt cgctacggat ttcagactac gcaggttaaa ggcggtcata aaggccacca
     4201 gcgccaccac aaacatccac gatggaatgg aaggcaccag agcttcaaaa tagattttcg
     4261 ccagcagaat gttgatcatc ggcgcgaaca gatagtcgag cagagaagac caacccacca
     4321 taaagccgac agtcgggcta atggatttct gggcgtaagt gtatgcagag ccagcagaag
     4381 gatagcggcg aaccagcttc ccgtagctca gagccgtaaa caggatcgca atcaatgcga
     4441 acgcataggc tgtcggcaca tgaccatccg tgaggcctga aacgatacca aatgtatcaa
     4501 acagcgtcat cggctgcata taggctagac ccatcattac aaccggaact aacgtaagcg
     4561 ttttacgcaa ttccacgcga gaggtgtttg gagtaacgtt atgcgacatg gttattctcc
     4621 tttacggtga taaccgtcgc gtaagcaaaa aattgcccca tttttttgga ttcctcagcg
     4681 acaacaactg tcgattttta gtaaatatct atccggtacg aagcccggcc tcttggtatg
     4741 aatgattggt ttgaagcaaa aaataaccga cgctaattaa acgtcggttt ttaatcattt
     4801 tttgacagcg gcgcattgtg cctaaatggg ggggaaatga caagagaatg agaggcttgt
     4861 cataataatt tttctttgaa tggctgattt tccgtcatca gattttgcat aaacaccggc
     4921 gatcgcacta tttgctaaaa tttcatctcg ccaccagccg ctggcaagcc ctgcggtctg
     4981 ttcattccag ccaatccaga caggttcaaa gagagtttgg gcgaccactt tcttctcgat
     5041 taacgcgcca ctatcgagaa aacgttgcgc cagataccgt ggtaaatagc cgcagccaag
     5101 gccgctaatt tgcagctcca gtttggtttt aaaatcgaaa acggtaatgg cttcttgttc
     5161 gtcgagaagt tgtgaggccg tagaagcgcc tgcctgcgca gtgtccccca ccacaatcgc
     5221 ccgatatcgt ttaatgatac gacggtttaa cggttcttct tcctgggcta acggatgatg
     5281 aggcgcgacc gcaaagactt gttcaagatc gcccaaccgc gagaaaccaa actcactgga
     5341 agatggtggc tcatgcatcg cgccaacgat aatatccgct cgcccctgag tcagggcatc
     5401 ccaggaacca ccgagtacgc cattgataaa cttcaggcgc gtcacgctat gatgttgata
     5461 aaaagcttca atgagaggcg caagcagaga aaaaggaaac gtgtcgtcca cgccaatcac
     5521 cagctcgttt tcccagcctt catgcagttt gatcgcctgc ttttccagtt ctcgcacggt
     5581 atgcagaact tcccgccctt tctctaataa catttttccg gtgcgggtga atttagcgcg
     5641 gtgtccgcta cgatcaagca attgaatatt taggtcgctt tccagcttgt gaacggtgta
     5701 actcagagcg gacggcgtct tgtagagttt ggctgacgcc gccgcaaaac tgccctcttt
     5761 ttctatggca tcgaggatca tcaacacatc cagcagtggt ttcatacacg cccccttgca
     5821 gtgcatgcgg cgtcctgctg gcgtgatcgt ttactccaac ggcattacca gcggatcggg
     5881 atactggtat tcaaatccca gttcattaca aatccgactg ccatcaataa tcttaccttt
     5941 gccgctgtcc agactatttc tgaactgcgg aggttgcagc cccagtaaac gggccatctg
     6001 cggatagaaa acattacgcg cagggtgagc tggcgcacat atattataga tgtgtccgcc
     6061 tttaggtgcc tgtaacaaca gagtgatagc gccaataaca tcttctaaat ggactaaatt
     6121 aacaccatgt tcaccatcag gggcggtttt tccggcaaag aagcgtcccg gatgacgtcc
     6181 cggtccgacc aggcccgcaa gacgcagaat atcgaccgaa gtaccgggta aattgtgcag
     6241 ccagtcttcg agttcttcta atactcgtcc actgttggtt accggattac gcggggtagt
     6301 ttctttaacc gtgccttgcg cgtcgccata gacagatgtt gagctggtaa aaataattcg
     6361 gggaatacga tgggccagcg cgctatccac taactcttgt accgcttgta aatagaactc
     6421 atcgccgggg ccgctacgac gtgccggaag cgtaatgacc agcgcatcgg catccataag
     6481 ggcatccaga tcgtcagaat cgcaaactaa ctcaggctcc atgcgaagca gatagctatc
     6541 aatgccactc attcgggccg cttcgacacc atcttgtgtg gttttactcc cggtgacttg
     6601 ccagcctcgc gctgaaagtg acatcgccag cggcatacct aaccacccta acccgacaat
     6661 tgcgaccttt ttcatccgtt atctcctgat acaccttaga tctataaggc tacgctagcg
     6721 tatcaaaact gacaattcat tctatgaatg aatctgttca ataatgataa cgacatgctg
     6781 caatgagcag tgaatcatca gtaaccgcgt ataccagacg gtgctcctct gtaatgcgtc
     6841 gggaccagaa acctgacaaa ttatgtttca ggggttctgg tttcccctta ccttcaaatg
     6901 gtgttctgcg ggtatctttg ataagttcat tgatcttttt aacaattcgc ttatctgttt
     6961 cctgccagta cagataatca tcccatgatt cctcagacca gattagtttc actca
//
LOCUS       DAJTQY010000148         6977 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.148, whole genome shotgun sequence.
ACCESSION   DAJTQY010000148 DAJTQY010000000
VERSION     DAJTQY010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6977
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.148"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(90..992)
                     /locus_tag="OO349_003653"
     CDS             complement(90..992)
                     /locus_tag="OO349_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HCR5868577.1"
                     /translation="MARLFTPSESKYYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWR
                     HLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIDPEYIAAVSACSMREGIVLYNNE
                     GTPIWACANVDARAAREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSD
                     IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLLDLTTRAWKPALLDMAGLRADIL
                     SPVKETGTLLGVVSSQAAELCGLKAGTPVVVGGGDVQLGCLGLGVVRPAQTAVLGGTF
                     TDNKQCTLSEARHQLPITPFRMPS"
     gene            complement(1071..2024)
                     /gene="lsrR"
                     /locus_tag="OO349_003654"
     CDS             complement(1071..2024)
                     /gene="lsrR"
                     /locus_tag="OO349_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HCR5868578.1"
                     /translation="MTINDSAISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            2273..3808
                     /gene="lsrA"
                     /locus_tag="OO349_003655"
     CDS             2273..3808
                     /gene="lsrA"
                     /locus_tag="OO349_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HCR5868579.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGLAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFSRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     DLSGKTSELSTDDIIQAITPAVREKSLSASQKLWLELPGNRPQHAVGTPVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNGKEINKLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGSNQQKILIAKCLEASPQALIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLFISSDMEEIELMADRVYVMHQGEITHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            3802..4830
                     /gene="lsrC"
                     /locus_tag="OO349_003656"
     CDS             3802..4830
                     /gene="lsrC"
                     /locus_tag="OO349_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HCR5868580.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGALVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLLGIS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFILNQTGTGLEMKAIAACVLGGISLLGGSGAFIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            4830..5822
                     /gene="lsrD"
                     /locus_tag="OO349_003657"
     CDS             4830..5822
                     /gene="lsrD"
                     /locus_tag="OO349_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HCR5868581.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            5834..6857
                     /gene="lsrB"
                     /locus_tag="OO349_003658"
                     /pseudo
     CDS             5834..6857
                     /gene="lsrB"
                     /locus_tag="OO349_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
     gene            6884..>6977
                     /locus_tag="OO349_003659"
     CDS             6884..>6977
                     /locus_tag="OO349_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="involved in autoinducer 2 transport and processing;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase LsrF"
                     /protein_id="HCR5868582.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFTLKGCGA"
BASE COUNT         1607 a         1768 c         1834 g         1768 t
ORIGIN      
        1 tagctaaatt gagatgtccg tctctgacta aatagacatg tccgcgctac agtctcacca
       61 cagcgatcac tgctttcaaa ggatgggact catgacggca tacggaacgg agtaatcggc
      121 aactgatgcc gggcctcgct gagcgtgcac tgcttattat ccgtgaacgt gccgccaaga
      181 accgcggttt gtgccggacg cacaacgcct aacccaaggc aaccaagctg cacgtcgcct
      241 cctccaacga ccaccggagt gcccgccttc agaccgcaga gttccgccgc ttgtgaactt
      301 accacgccca gcaatgtgcc ggtttctttg acaggagaaa gaatatcggc acgcaggcca
      361 gccatatcca gcaatgcagg tttccaggca cgggtggtta gatcaagaag tcccgtggtg
      421 ccagcgttag agggatccac cgccagttcg ccgctgagca tataggccag ccagtcgctg
      481 atcatggtga tggttgatgc ctgacggtaa atatcggaac gatggtgcgc cagccaaagt
      541 aatctgggga tggcacttaa agccagtgtt tgtccggtcg ctcgataaac ttcgttttca
      601 aaggtattgt tgtgcagttc tttaagttcg ctaacttcgc gtgccgctct ggcatccaca
      661 ttggcgcagg cccagatcgg tgttccttca ttgttatata aaacaatgcc ttcacgcata
      721 gaacatgccg aaacggcagc gatatactcc gggtctatgc cggcgttgtg cagcgcctgg
      781 cgcatacact cacacgccag ttgccagttt ttgttgagat caaattccat agaaccagga
      841 acgtccggta ctgccagatg ccgccactcc gcctgtccca ctgctatttg attgccttcc
      901 aggtcgaata tcacagcccg aatacttccg gtgcctgcat ccagcgccat caggtagtac
      961 tttgattctg aaggggtaaa gagtcgagcc attagattat cctcggctta tgcgttctgt
     1021 ctgttcctct atacgttctc catcattccc ggtaataagg tcatgcaaat ttaactacgt
     1081 aaaatcgccg ctgctgtgtc ctgatcggta accagtgcgt tgatataacc gcctttcatt
     1141 gcagcggcaa ttgcttcggc tttattttct ccccctgcca cgccaacccg gacgggtatg
     1201 gtcttcagcg cgcttaaagg taagccaatc agttcgttat gtattttgat atccgtgaca
     1261 acgtcacctt ttgcatcaaa aaagtagcct aaaatgtcgc caaccgcccc ttttcggcca
     1321 atcattaact gttcgccctg gctgatataa ccggagcgaa tgattgtcgc gtcgtcttgt
     1381 tgactcacgg ctccaatgcc gacaatcgcc acatccgctg cttgcgcggc tagcagaaca
     1441 tctttgacgc aattttcatt ttttagcgta cgggcaatgt cagcggagga tgcccgcaac
     1501 ggagccggaa taatattcac actgcacgcc gcgttaagct gcccgatgcc cgtcatataa
     1561 gaaccgacgc caccggagag cgtgaccagg cgaatttgct gtgacgaaat aaaaccactt
     1621 aagcgttgca gcgtattcat ggttgcctcg ccaaaaccaa tcgccagcat ctgttgtggt
     1681 tgaagtaaac tcatcaacat atgcgccgcg cctatcccta gtcgcccgcc gacatcagca
     1741 tccgcaagcc cagggatcac ccggacatgt tgcagcgaaa actgacgacg taattgagtt
     1801 tcatattcca gacagccttc aaagcgagaa ttaatctgta cgcgaataat gccggactga
     1861 tgccctttct ccagcaagcg cgacactttc aaacgggtca ggccgagacg atcgctgatc
     1921 tcgctctggg tcagcccgtc gtgatagtaa aaccacgcga tccgcgcgac ctgttcttct
     1981 tcacacattc cctgttctga aattgccgaa tcgttgattg tcataattca ttcttcactt
     2041 tgaacatatt taaatcttta atgcaattgt tcagttcttg cgcatttata tctgtgatgg
     2101 caaccacagt ttgactctac gagcatgaac aaacgcaacc gtgaaaatca aaatagcata
     2161 aattgtgatc tattcgtcgg aaatatgtgc aatgtccacc taaggttatg aacaaattaa
     2221 aagtagaaat acatttgttc aaaactcacc tgcaaaactg aacgggggaa atatgcaaac
     2281 gagtgatacc cgcgcgttac cgctactttg cgcccgctcg gtttataaac agtattcagg
     2341 ggtcaatgtc ctgaaaggca tcgattttac gttgcatcag ggggaggtcc acgccctgct
     2401 cggcggcaat ggtgccggta aatcgacgtt aatgaagatt attgccggta ttacccctgc
     2461 tgatagcggt acgctggaga ttgggggcaa caactacgcc agattaacgc cagttcatgc
     2521 tcatcagctg ggcatttatc tcgttcccca ggaaccgctg cttttcccaa gcctgtcgat
     2581 aaaagaaaac atcctgtttg ggctggcaaa aaaacagctc tccatgcaga aaatgaagaa
     2641 cttgctggcg gcgctgggct gccagtttga tctgcatagt ctggcaggat cgctggatgt
     2701 cgccgatcgc caaatggtgg aaatcctccg cgggctgatg cgcgactcgc ggattctgat
     2761 cctcgatgaa cctaccgcct cgcttacccc tgcggaaacc gaacgcttgt ttagtcgctt
     2821 gcaagagctg cttgctactg gcgtgggtat tgtttttatc tcgcataagc tgccggaaat
     2881 tcgccagatt gccgatcgaa ttagcgtgat gcgcgacgga accatcgact taagcggcaa
     2941 aaccagcgaa ctgtctaccg acgacattat tcaggccatc accccagcgg tacgggaaaa
     3001 atcgctctct gccagccaaa aattatggct ggagttacct ggtaaccgcc cacaacatgc
     3061 cgtcggaacg ccggtgctga cactggaaaa tctgactggc gaaggcttca ggaatgtcag
     3121 cctgacgctc aatgccggag aaattctggg cctggctggg ctggtgggag ccggacgcac
     3181 agaactggcc gagacgctct atggtctgcg tactttgcgt ggcggacgca ttatgctgaa
     3241 tggtaaagag atcaataaat tatccactgg agaacgttta ctgcgcggtc tggtttatct
     3301 gccggaagat cgccagtcat ccggactgaa tctcgatgct tcgctggcct ggaacgtctg
     3361 cgcccttact cataaccttc gtggattctg ggcgaaaacc gcgaaagata atgccactct
     3421 ggaacgttat cgtcgggcgc tgaatattaa atttaaccaa ccggaacaag ctgcacggac
     3481 attatccggt agcaaccagc aaaaaatcct cattgccaaa tgcctggaag cttcgccgca
     3541 agcattgatt gtcgatgagc cgacgcgcgg cgtggatgtc tcggcccgta atgatatcta
     3601 ccagctgttg cgcagcatcg ccgcacagaa tgtggctgtg ctgtttatct cctctgatat
     3661 ggaagagatc gaactgatgg cagatcgtgt gtatgtgatg catcagggcg aaattaccca
     3721 ctctgcactg accgggcgcg atattaatgt cgagactatt atgcgcgttg ccttcggcga
     3781 tagtcagcgt caggaggcat catgctgaag tttattcaga acaaccgtga aatcacggca
     3841 ttgctggcgg tggtgctgct gtttgtatta cccggttttc tcgaccgcca gtatttaagt
     3901 gtgcaaacgc tgaccatggt ttatagcagc gcgcaaatcc tgatcctgct ggcaatgggc
     3961 gcgacgctgg taatgcttac gcgcaatatt gatgtttcag tgggttcgat taccggaatg
     4021 tgcgcggtgc tgttggggat gttactgaac gcaggatatt cactacctgt tgcctgtgtc
     4081 gcgactttac tgcttggttt gctcgcggga tttttcaacg gtgccctggt cgcgtggcta
     4141 aagatccctg ccattgttgc cacccttggc acgttagggt tgtacagagg catcatgttg
     4201 ctgtggactg gcggtaaatg gattgaaggg ttacccgccg aactgaaaca gctctccgcc
     4261 ccgctgctgc ttggcatttc agcaattggt tggttgacga taattctggt ggcatttatg
     4321 gcctggctgc tggcgaagac ggcgtttgga cgcagttttt atgccacggg cgataattta
     4381 cagggcgctc gtcaactggg cgttcgtacg gaagccattc gcattgtggc attttcgttg
     4441 aacggctgca tggcggcact ggcgggaatt gtgtttgctt cgcagattgg ttttatcctc
     4501 aaccagaccg gtaccgggct ggagatgaaa gcaattgcag cctgcgtgct gggcggcatt
     4561 agtttgctcg gtggttccgg tgcgttcatc ggtgcggtac tcggcgcatg gttcctgacg
     4621 cagatcgata gcgtactggt gctgttgcgc attccggcat ggtggaatga ttttatcgcg
     4681 ggtctggttc tgctggcggt gctggtgttt gatggacgcc tgcgttgtgc tctggaacgt
     4741 aatctacggc ggcaaaaata tgcccgcttt atgacgccac cgccatccgt taaacccgct
     4801 tcgtcaggta aaaaacggga ggccgcataa tgcgtattcg ctacggttgg gaactggctc
     4861 ttgccgcact gctcgttatt gagattgtcg catttggtgc aattaacccg cgaatgttag
     4921 atctcaatat gttgctgttc agcaccagtg actttatctg cattggcatt gtcgctctac
     4981 cgctgacgat ggtgattgtc agtggcggga tcgatatttc gtttggttcg accatcggcc
     5041 tctgcgccat tgcattgggc gtactgtttc aaagtggtgt gccgatgccg ctggcgatac
     5101 tcctgacctt actgctcggc gcattgtgcg ggctgatcaa cgccggatta attatctata
     5161 ccaaagttaa cccgctggtg attacgcttg gcacgctgta tctgtttgcc ggaagcgctc
     5221 tgctgctttc cggtatggcc ggagcgacgg ggtacgaagg tattggtgga ttcccgatgg
     5281 cgtttacaga tttcgctaac ctggatgtgc tgggactccc cgttccgctg attatcttcc
     5341 tgatatgtct cctcgttttc tggctctggc tgcataaaac ccatgccgga cgtaatgtgt
     5401 ttttgattgg gcaaagcccg cgcgtggcgc tttatagcgc gattccagtt aaccgcacct
     5461 tatgtgcgct ctatgccatg acggggctgg cgtctgcggt cgccgctgtg ctgctggtat
     5521 cgtattttgg ttcagcacgt tccgatctcg gtgcgtcgtt tctgatgccc gccatcaccg
     5581 ccgtggtgct tggcggggcc aatatttatg gtggttccgg ttccattatc ggcaccgcca
     5641 ttgcggtttt attagtggga tatttgcagc aaggtttgca aatggcagga gtgccaaatc
     5701 aggtgtccag cgccctttcc ggtgcgctgc ttatcgtcgt tgtcgtaggt cgttccgtta
     5761 gcctgcatcg ccagcaaatt aaagagtggc tggcgcgtcg ggccaataac ccattgccat
     5821 aaaggatatc ttcatgacac ttcatcgctt taagaaaatc gccttactta gcgctcttgg
     5881 cattgccgca atctctatga atgtgcaggc cgcagagcgt attgcattta ttcccaaact
     5941 ggttggcgtg ggatttttta ccagcggtgg caacggcgca caacaagcgg gtaaagagct
     6001 gagcgttgat gtgacctacg acgggccgac agaacccagt gtttctggtc aggtacagtt
     6061 gattaataac ttcgtcaatc aaggttataa cgccattatt cgtttctgcg gtttcgcctg
     6121 atggcttgtg tccggcactg aaacgcgcca tgcaacgtgg tgtgagagtg ctgacctggg
     6181 actctgatac taaaccggag tgccgctctt actacattaa tcagggaacg cccgcccagt
     6241 tgggaggtat gttggtggat atggcggcgc gtcaggtgaa taaagacaaa gccaaagtcg
     6301 cgtttttcta ctcaagcccc accgttacgg accaaaacca gtgggtgaaa gaagcgaaag
     6361 cgaaaatcgc caaagagcat ccaggctggg aaattgtcac tacgcagttt ggctataacg
     6421 atgccactaa atcgttacaa accgcagaag gaatattaaa agcgtatagc gatctcgacg
     6481 ccattatcgc ccccgatgcc aacgccctgc ccgctgccgc acaagccgca gaaaacctga
     6541 aaaatgacaa agtagcgatt gtcggattca gtacgccaaa tgtgatgcgc ccgtatgtag
     6601 agcgcggcac ggtgaaagaa tttggactgt gggatgtggt tcagcaaggc aaaatttcag
     6661 tgtatgtcgc ggatgcatta ttgaaaaaag gatcaatgaa aacgggcgac aagctggata
     6721 tccagggcgt aggtcaggtt gaagtctcgc caaacagcgt tcagggctat gactacgaag
     6781 cggatggtaa tggcatcgta ctgttaccgg agcgcgtgat attcaacaaa gagaatatcg
     6841 gcaaatacga tttctgatgt gcattactta accggagtaa gttatggcag atttagacga
     6901 tattaaagat ggtaaagatt ttcgtaccga tcaaccgcaa caaaatatcc cttttaccct
     6961 gaaaggttgc ggtgcgc
//
LOCUS       DAJTQY010000149         6951 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.149, whole genome shotgun sequence.
ACCESSION   DAJTQY010000149 DAJTQY010000000
VERSION     DAJTQY010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6951
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.149"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(225..1067)
                     /gene="purU"
                     /locus_tag="OO349_003660"
     CDS             complement(225..1067)
                     /gene="purU"
                     /locus_tag="OO349_003660"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HCR5868583.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            complement(1117..1575)
                     /locus_tag="OO349_003661"
     CDS             complement(1117..1575)
                     /locus_tag="OO349_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707139.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HCR5868584.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMTGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            1688..2593
                     /gene="rssA"
                     /locus_tag="OO349_003662"
     CDS             1688..2593
                     /gene="rssA"
                     /locus_tag="OO349_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HCR5868585.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            2685..3698
                     /gene="rssB"
                     /locus_tag="OO349_003663"
     CDS             2685..3698
                     /gene="rssB"
                     /locus_tag="OO349_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HCR5868586.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            3900..4808
                     /gene="galU"
                     /locus_tag="OO349_003664"
     CDS             3900..4808
                     /gene="galU"
                     /locus_tag="OO349_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCR5868587.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(4952..5365)
                     /gene="hns"
                     /locus_tag="OO349_003665"
     CDS             complement(4952..5365)
                     /gene="hns"
                     /locus_tag="OO349_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HCR5868588.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            5970..6587
                     /gene="tdk"
                     /locus_tag="OO349_003666"
     CDS             5970..6587
                     /gene="tdk"
                     /locus_tag="OO349_003666"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HCR5868589.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
BASE COUNT         1833 a         1524 c         1700 g         1894 t
ORIGIN      
        1 gaaccttcga agtcgatgac ggcagattta cagtctgctc cctttggccg ctcgggaacc
       61 ccaccacggg gtaatgcttt ttactagcct gctcccttat cgggaagcgg ggcgcatcat
      121 atcaaatgac gtgccgctgt aaagtgttac gttgagaaaa atgaactggt tgcgtaattt
      181 tcatccgtaa cggattaaag gtaaccagtt atttttgctg gcgattaaag aataatcgtc
      241 cgattaccgt aaacaaagac gcgttgcgcc agtactttgt atagcgcacg acttaagacg
      301 tttttctcga cgtcacgacc tgcgcgcatc atatcttcag ctgtgtaggt atgatcgaca
      361 tgaataacgt cctgcatgat gattgggcct tcgtccagat tgtcattcac atagtgagcg
      421 gttgcgccaa taatcttcac accacgttca taggcctggt gataaggacg tgcgccgata
      481 aacgcaggca ggaatgaatg gtgaatattg atgattttat tcgggaagcg tgccacaaat
      541 tccggcgtta atacccgcat atacttcgcc agcaccacat agtcaggttg ataagcatca
      601 atggcgtccg ccatcttttg atcgtgctcg ttgcggctta acccttcatg gcttaccagc
      661 tcaaacggaa tatcaaaacg ctcaaccaga gaacgtaaag tatcgtggtt accaataact
      721 gccgcgattt cgacatccag accgccgtaa ttggctttca tcaacaaatc gccaaggcaa
      781 tgcgcttctt tagtgaccag aatcactatc cggcgacgac cggcaggatt cagctcacgc
      841 acggagcctt ctggcaatgc gctatcgaga tccgccagca gggtggaatc attaaaaatc
      901 ccttccagtt ccgtgcgcat aaaaaagcgc ccggtacggt gatcaacaaa ttcattgttc
      961 tgtacgatat ttaactcgtg cttgtagcaa atattggtaa tacgtgcgat cagacctttt
     1021 tggtccggac aaatagtacg cagaacttta cgttggagtg aatgcattgc tggaaaaacc
     1081 ttgttgagag tgtttgctaa accgtaccgt caaccattat tggccgcagc actttttaaa
     1141 ttttttacct gaaccacaag ggcagggatc gttgcgacca aactgcggac gtgtaccgtc
     1201 aatatagtac cattgaccgt tttctttcaa aaatcgcgaa cgttcaatta ttgcacctgt
     1261 tttgccacct tcggtaaatc gagcaacaaa actgacaaat ccgatattgt cggcgtcctg
     1321 ccaacaatgt tcaaatacgg tcaatcccaa ccattcagta tgcgcaaatc cggtcatcaa
     1381 ttcggcacgt aacgccgctg ctccacaaga gggatgccag gtctttatta aataatcagc
     1441 gtcttgcatc acaaaagcgc agtaacgcga acgcatgaga tgttctggat caggtgcaac
     1501 cttttcacca gacacataag ggtggcaaca taggctatac tcgacagcac taccacaggg
     1561 acaaagctga gacacaaata atctccctgg aaacaataac ggcgtattaa ccgcctgagt
     1621 agcactatgt taaccgagca gtagcgatgt ggctacgatt gcattccagg ggaatcttgc
     1681 gggaataatg agaaagataa aaatagggct ggcgctggga tctggcgcgg cgagaggttg
     1741 gtcgcatatt ggcgttatta atgcgctaaa aaaagtgggt attgaaattg atatcgttgc
     1801 aggatgttca attggttcgc tggtgggcgc ggcctatgca tgcgatcgat tatctgcgct
     1861 ggaagattgg gtaacctctt tcagttattg ggacgtttta cgcctgatgg acctctcctg
     1921 gcagcgcggt gggttactgc gcggcgagcg tgtcttcaat caatatcgcg aaataatgcc
     1981 ggaaacagag atcgaaaatt gttcccgtcg ctttgcggct gttgccacca atttaagtac
     2041 ggggcgtgaa ttatggttta ctgaaggcga tctccatctt gctattcgcg catcatgcag
     2101 tattccagga ctcatggcac ccgttgcaca taacggctac tggctggttg atggggcagt
     2161 agttaaccca attcctattt ctcttacgcg tgcattgggg gctgatattg tgatagcggt
     2221 cgacctgcag cacgatgctc atttgatgca acaagatctg ctctccttta atgtcagtga
     2281 agaaaatagc gagaatggtg attctctacc gtggcatgcg cgtctgaaag aaaggttagg
     2341 cagcataacg acacgtcggg ctgtgacagc gccaacggca acagagatta tgaccacgtc
     2401 tatccaggtg ttggagaacc gccttaaaag gaaccgcatg gcaggtgatc cgcccgatat
     2461 tctgattcaa cctgtttgcc cgcaaatatc tacgcttgat ttccatcgcg cgcacgctgc
     2521 cattgcggcc ggacagctgg cagtggaaag gaaaatggac gaacttttgc cgttggtacg
     2581 caccaacatt tgaccagaat ttttatctac acttaagtta attctgacag gcgcaggtgg
     2641 caatagcatg ccactattga gtaaagccag tcaggggaga gaacatgacg cagccattgg
     2701 tcggaaaaca gattctcatt gttgaagatg agcaggtatt tcgctcgctt ctggattcat
     2761 ggttttcctc attgggagcg acaacggtac tggcggctga tggggtggat gcccttgagt
     2821 tgctgggagg tttcactcca gacctgatga tatgtgatat cgcgatgcca cgaatgaacg
     2881 ggcttaaact gctggagcat atacgtaaca gaggcgacca gaccccagtt ctggtgatat
     2941 ctgccactga aaatatggca gatattgcca aagcgttacg tctgggcgtt gaagatgttt
     3001 tgctgaaacc agttaaagat ctgaatcgct tgcgcgagat ggtttttgcc tgtctctatc
     3061 ccagcatgtt taattcgcgc gttgaggaag aggaaaggct ttttcgcgac tgggatgcaa
     3121 tggttgataa ccctgccgca gcggcgaaat tattacagga actacaaccg ccggttcagc
     3181 aggtgatttc ccattgccgg gttaattacc gtcaattggt tgccgcggac aaacccggcc
     3241 tggtgcttga tattgccgca ctttcggaaa acgatctggc attttattgc cttgatgtca
     3301 cccgagctgg acataatggc gtacttgctg ccttgttatt acgcgcattg tttaacggat
     3361 tattacagga acagcttgca caccaaaatc aacggttgcc agagttgggc gcgttattga
     3421 agcaggtaaa ccatttactt cgtcaggcca atctgccggg gcagtttccg ctattagttg
     3481 gctattatca tcgcgaactg aaaaatctca ttctggtttc tgcgggtctg aatgcgacgt
     3541 taaataccgg cgaacaccag gtgcaaatca gtaatggtgt tccgttaggc actttaggta
     3601 acgcttattt gaatcaattg agccagcgat gcgatgcctg gcaatgccaa atatggggaa
     3661 ccggtggtcg actgcgcttg atgttgtctg cagaatgagc aaacgataac gcgggctaaa
     3721 tttgcattac ctgctaatgt cggctggtgg tactatcgtc gccattcgta taagtaattg
     3781 tcttaattat gctatctcgc ctccttttca gaacttagcc ccttcggggt gctgatatac
     3841 tgggatgcga tacagaaata tgaacacgtt caaaacacga acagtccagg agaatttaaa
     3901 tggctgccat taatacgaaa gtcaaaaaag ccgttatccc cgttgcggga ttaggaacca
     3961 ggatgttgcc ggcgacgaaa gccatcccga aagagatgct gccacttgtc gataagccat
     4021 taattcaata cgtcgtgaat gaatgtattg cggctggcat tactgaaatt gtgctggtta
     4081 cacactcatc taaaaactct attgaaaacc actttgatac cagttttgaa ctggaagcaa
     4141 tgctggaaaa acgtgtaaaa cgtcaactgc ttgatgaagt gcagtctatt tgtccaccgc
     4201 acgtgactat tatgcaagtt cgtcagggtc tggcgaaagg cctgggacac gcggtattgt
     4261 gtgctcaccc ggtagtgggt gatgaaccgg tagctgttat tttgcctgat gttattctgg
     4321 atgaatatga atccgatttg tcacaggata acctggcaga gatgatccgc cgctttgatg
     4381 aaacgggtca tagccagatc atggttgaac cggttgctga tgtgaccgca tatggcgttg
     4441 tggattgcaa aggcgttgaa ttagcgccgg gtgaaagcgt accgatggtt ggtgtggtag
     4501 aaaaaccgaa agcggatgtt gcgccgtcta atctcgctat tgtgggtcgt tacgtactta
     4561 gcgcggatat ttggccgttg ctggcaaaaa cccctccggg agctggtgat gaaattcagc
     4621 tcaccgacgc aattgatatg ctgatcgaaa aagaaacggt ggaagcctat catatgaaag
     4681 ggaagagcca tgactgcggt aataaattag gttacatgca ggccttcgtt gaatacggta
     4741 ttcgtcataa cacccttggc acggaattta aagcctggct tgaagaagag atgggcatta
     4801 agaagtaaca tccgtatcgg tgttatccac gaaacggcgt tgagcaatcg acgccgtttt
     4861 tttatagctt attcttatta aattgtctta aaccggacaa taaaaaatcc cgccgctggc
     4921 gggattttaa gcaagtgcaa tctacaaaag attattgctt gatcaggaaa tcgtcgaggg
     4981 atttaccttg ctcatccatt gcttttttga ttacagctgg agtacggcct tggccagtcc
     5041 aggttttagt ttcgccgttt tcgtcaacgt agctatattt tgccggacgc tgagcacgtt
     5101 tagctttggt gccagattta acggcagcaa ggctattcag cagttcgttc gggtcaatac
     5161 cgtcagcgat cagcatttcg cgatattgct gcagcttacg agtgcgctct tcaacttcag
     5221 cagcagccgc gctttcttct tcgcgacgtt cgttaacaac aacttctaat ttttccagca
     5281 tttcttccag cgtttcaagt gtacattctc ttgcctgcgc acgaagagta cggatgttgt
     5341 tcagaatttt aagtgcttcg ctcattgtag taatctcaaa cttatattgg ggtggtttgt
     5401 tgaggtaata atagagcctt aaattcagtt gtgcaatagc caggaatgta aggaattcaa
     5461 aattgttctt tattttgtgc cgccaataaa tatcttttca taaaattagc cagaaaagac
     5521 gcggcatata gccctattta caccgatgat ttcgcagcac gtgaggttaa aacttcctga
     5581 ttcatgtcac attttatggg gagattatcg taggctgacg acctttcagt cttctgtatt
     5641 agttgtgttt acgagaattc cctattaagc gaatgatgaa aagtagaaca gtcgcaataa
     5701 gagcatggac ttagtattgc actatctcct ggaggtcaac agagggctat tacttgcgca
     5761 acaggttaaa gattgtgaat agttaccagc agtcatttac ccgcttataa caagcgaggc
     5821 agttgtaatg atagctcaga aggattatgc aaggcttcgt aagggagaac gcatataccc
     5881 acttctgtgc atactgttga gctgaaaaac tgacgaatta tgataaactc cagccaactt
     5941 tatttcatat cattgagggc ctgtggctga tggcacagct atatttctac tattccgcaa
     6001 tgaatgcggg taagtctaca gcattgttgc aatcttcata caattaccag gaacgcggca
     6061 tgcgcactgt cgtatatacg gcagaaattg atgatcgctt tggtgccggg aaagtcagtt
     6121 cgcgtatagg tttgtcatcg cctgcaaaat tatttaacca aaattcatca ttatttgatg
     6181 agattcgtgc ggaacatgaa cagcaggcaa ttcattgcgt actggttgat gaatgccagt
     6241 ttttaaccag acaacaagta tatgaattat cggaggttgt cgatcaactc gatatacccg
     6301 tactttgtta tggtttacgt accgattttc gaggtgaatt atttattggc agccaatact
     6361 tactggcatg gtccgacaaa ctggttgaat taaaaaccat ctgtttttgt ggccgtaaag
     6421 caagcatggt gctgcgtctt gatcaagcag gcagacctta taacgaaggt gagcaggtgg
     6481 taattggtgg taatgaacga tacgtttctg tatgccgtaa acactataaa gaggcgttac
     6541 aagtcggctc attaacggct attcaggaaa ggcatcgcca cgattaataa gaatttcttt
     6601 actgacaggg tgagcagggc acttttatcc tgtcagttcg ttttacgcac ttcttccggg
     6661 ctatataccc ttctcggcag ttttttaacg ccgctatacg cctcacaggg ctcttaagca
     6721 ccgacgttga cttgtgacct gtaaagtaca atatccctgt gtttaggcgt tatacatcgt
     6781 cgcaaatatg atgaaggcta atgctgtcgg tttatggaaa agttgctttg ggtaaacaaa
     6841 aaaacggccc cagaaggggt aatgccagtc agttaagcaa ctgactggct ctttttcgga
     6901 gctgtggggt atttccaggg cctctccttt accactctcg ggaaggccct t
//
LOCUS       DAJTQY010000150         6913 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.150, whole genome shotgun sequence.
ACCESSION   DAJTQY010000150 DAJTQY010000000
VERSION     DAJTQY010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6913
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.150"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(210..590)
                     /locus_tag="OO349_003667"
     CDS             complement(210..590)
                     /locus_tag="OO349_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCR5868590.1"
                     /translation="MRDIQQVLERWGAWVANNHEDVTWSSIAAGFKGLIPSKVKSRPQ
                     CCDDDAMVICGCMARLNRNNNDLHDLLVDYYVFGMTFMTLARKHGCSDGYIGKKLQKA
                     EGVVEGMLMMLGVRLEMDKYIEHP"
     gene            complement(583..783)
                     /locus_tag="OO349_003668"
     CDS             complement(583..783)
                     /locus_tag="OO349_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000111769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninH"
                     /protein_id="HCR5868591.1"
                     /translation="MSVKIQTIPELLIQTRGNMTEVSRMLNCNRATVRKYAEDKEGKG
                     HAIVDGVLIVHRGWDRGKDSDA"
     gene            complement(878..1135)
                     /locus_tag="OO349_003669"
     CDS             complement(878..1135)
                     /locus_tag="OO349_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5868592.1"
                     /translation="MNNQTMTFTPEQLRKHAQEMLRHAEQLEKTGITKDAIRKDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETRVGKFEKLVQEALR"
     gene            complement(1132..2523)
                     /locus_tag="OO349_003670"
     CDS             complement(1132..2523)
                     /locus_tag="OO349_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCR5868593.1"
                     /translation="MTTPVWRNDDLEGAVIGAFFLRGADPEVMDILATLPADVFSVRA
                     YQDIYTGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALRRNA
                     ALRDAEKTLNEALQKLRDAHTCEAAEDALKDAQNMMVTLSTGKGVIQPVHIDDVLPEV
                     VERVECRNQGLEKSRTLMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDIIDKV
                     TEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVAHFEDEDWTRFSQGVGRMTG
                     RNIWMVDQANLAIDEICATTKHHLIKYPETALVVVDYLGLIKTRTTGRHDLAVGEISK
                     GLKGLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRDEVY
                     NPDTQARGIAEINITKQRNGSLGTIYRRFYNGHFLPVDQESARVLSTPMKPGNPRRYS
                     NKRTDSSKMERFF"
     gene            complement(2520..3398)
                     /locus_tag="OO349_003671"
     CDS             complement(2520..3398)
                     /locus_tag="OO349_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR5868594.1"
                     /translation="MMTFNLREQQKRLQARMDELRAEIAFAQKGEKPWPYRSCLMREG
                     RGYCEKHGEYHTHILVWSDRNGEDREEISCCPDCLIAEANDLTMELSSIKAEELTDNA
                     GIALRFRDCEFDNYLEVNPGAARNLAACRRYAENWPDMLENGTSLVMTGSCGTGKNHL
                     AVAMAKHIIRNYLASVEITDVMRLTRAVKNCWRNDSEKTADEVIERYASMDLLIIDEV
                     GVQFGSAAEMAILQEIINARYESILPTILISNLSPEELWAFISPRIADRITDGGRNWL
                     SFNWPSYRSRIRGVAA"
     gene            complement(3409..4317)
                     /locus_tag="OO349_003672"
     CDS             complement(3409..4317)
                     /locus_tag="OO349_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR5868595.1"
                     /translation="MRPSDLLLDFGHPVAYYPGLVKYMGSPHAVIFFGQIFYWQDKAH
                     AAEGVHKTREEIQHETGLTFEQQAVARKHLVSRGILVETNKRLEHKMFYRIDCERLNE
                     IINENNQFSRNGETRFRETVKPNFAEEGKPSPRTRETPRRGEGKTNFDLTENTTEITS
                     ENTTESKNTIGASADASAPARSSRQEYSPEFEQAWQEYPKRAGGNSKSAAFKAWKARL
                     REGIKPEAMLDGVKRYAAWVRATGNTGTQFVKQAATFFGPDRHFEDFWQQPAAPRGGR
                     QRQVDVLAGLGAMSDKFGKSSDKLTF"
     gene            complement(4304..4537)
                     /locus_tag="OO349_003673"
     CDS             complement(4304..4537)
                     /locus_tag="OO349_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000621187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5868596.1"
                     /translation="MIRHIVNFLYHRYNHCPRVGQWFTTSNGYVLRVCLVSTESQKVV
                     CQVQGRTHTLSYPLVAFQPGKMFKRLGGGYASV"
     gene            complement(4534..5550)
                     /locus_tag="OO349_003674"
                     /pseudo
     CDS             complement(4534..5550)
                     /locus_tag="OO349_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005114998.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
     gene            5482..5745
                     /locus_tag="OO349_003675"
     CDS             5482..5745
                     /locus_tag="OO349_003675"
                     /inference="COORDINATES: protein motif:HMM:NF017757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCR5868597.1"
                     /translation="MRARYQHLVERMKVYGSNLGEPHTSAFGDGLFELRIKGNDGIAR
                     VFYCTLTGKRIIMLHSFVKKAQKTPPAERKKAETRMKEVKHDW"
     gene            5735..6043
                     /locus_tag="OO349_003676"
     CDS             5735..6043
                     /locus_tag="OO349_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5868598.1"
                     /translation="MTGKRTLPTMTHDEMAAKWMEDPAFKAEYDAIADEFALLDEMLA
                     ARKEAGLTQAEVAERMGTKATAITRMESNLASGISGPSFATLKKFARATGKKLQIRFV
                     "
     gene            complement(6085..6261)
                     /locus_tag="OO349_003677"
     CDS             complement(6085..6261)
                     /locus_tag="OO349_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HCR5868599.1"
                     /translation="MRRHRRSFTDIICENCKYLPTKRSRNKRKPIPKESDVKTFNYTT
                     HLWDIRWLRHRARK"
     gene            complement(6258..6668)
                     /locus_tag="OO349_003678"
     CDS             complement(6258..6668)
                     /locus_tag="OO349_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HCR5868600.1"
                     /translation="MKQTIFLRTKQQQQAAINAILSTPLDKDKPVTIRITDYKRNLDQ
                     NAKFHAMLADIARQVQWCGKWLKPEQWKVLLISGHAVATKQEADVLPGLEGECVNIRE
                     SSAQMSVKRMASLIEYTTAWAIGQGVRFTDRRYE"
     gene            complement(6665..>6913)
                     /locus_tag="OO349_003679"
     CDS             complement(6665..>6913)
                     /locus_tag="OO349_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001000126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5868601.1"
                     /translation="WWSKDKSQFTGDDELKEFAWVIWQASRAAIELDIDWPESNDDFW
                     KDGEEGAYAMGHEDGKDKTVIAVMKAIRAAGIKEKNFR"
BASE COUNT         1655 a         1869 c         1588 g         1801 t
ORIGIN      
        1 ctaaagggcc ggaggcagaa taataaccat atgtcatcac atctgcaaac tcatctgacc
       61 accagcgtgt ttaacgtcct gtaccgtttt tcaggcataa aaaacccgcc cggaggcggg
      121 tttaagctgt gtggcgaagc aaccactctt aacagaatat cccgtttttt acgtacgtaa
      181 aatattttct agaaagtcgc cccttaccat cagggatgtt caatatattt gtccatttct
      241 aaccggacac ccaacatcat caacattcct tccaccaccc cctcggcttt ttgtagcttc
      301 tttcctatat aaccatctga gcatccatgc ttccgcgcca gcgtcatgaa tgtcatccca
      361 aacacgtagt agtcaaccag caagtcatgt agatcgttgt tgttccggtt aaggcgggcc
      421 atacacccac atataaccat cgcgtcatcg tcacagcatt gcgggcggga ttttactttt
      481 gaagggatta atcccttaaa accggcagca atggacgacc aggtcacatc ctcatgatta
      541 ttagccaccc acgcgcccca gcgctcaaga acctgctgaa tatcacgcat cactgtcttt
      601 acccctgtcc catccacgat gaacaatcag aacaccatca acaatggcgt gccctttgcc
      661 ctctttatct tcagcatatt ttcttaccgt ggcgcgatta cagttcagca ttcgtgaaac
      721 ttcggtcata ttacctcgtg tctggataag cagttccggt atcgtttgaa ttttgacgct
      781 catcaaatac tctccggttc ggtgattttt atcccgggct ttccaccagg gacataatga
      841 tcgcgcacaa gccagggcaa aacaaactca tggcgcatca gcgcagcgcc tcctgcacca
      901 gtttttcaaa ctttccgact ctggtttcca gctctgccac acagtccacc agctcatcta
      961 ctgctttctg tgcgcgatgc ttcgcctgca tcagttcccg aagcgcgggt accatatctt
     1021 tacggatagc atcttttgtt atgcccgttt tttcgagttg ttcagcatga cgcagcattt
     1081 cctgcgcgtg tttacgcaat tgttcagggg taaaagtcat tgtctggttg ttcaaaagaa
     1141 acgctccatc ttactgctgt cagttcgttt attactgtat ctgcgcggat tgccgggctt
     1201 catgggagtg gaaagcaccc gtgcactttc ctggtccaca ggcagaaaat gtccgttata
     1261 aaaacgccgg taaatcgttc ccagagaacc gttacgttgt ttcgtgatat tgatttctgc
     1321 gatgcccctg gcctgcgtat ccgggttgta cacttcatcc ctgtaaagca tcagaatgat
     1381 gtctgcatcc gcctctattt ctccggaatt tttcaggtct gagttcatgg gacgtttatt
     1441 gggtctggac tccacaccgc gggagagctg gctcagcgca atcaacggaa aaccaccgga
     1501 ttttgccagg cctttaagcc cctttgagat ttcacccacg gcaaggtcat gacgccccgt
     1561 ggttcgggtt tttatcagcc cgagataatc aaccaccacc agcgccgttt ccggatattt
     1621 aatcaggtgg tgtttcgttg ttgcgcatat ctcatcaatg gccaggttcg cctggtccac
     1681 catccagata ttgcgcccgg tcatccgccc caccccttgt gagaaacgcg tccagtcttc
     1741 atcttcaaag tgagccacag atttcaggcg tgatactggc atccctccag ccgcagacac
     1801 catacgttca ccaatctgga tgttcgccat ctccatggtg aacagaagca caccatgccc
     1861 ctgctcagtc accttgtcga tgatgtccag cgcaagttcg gttttcccca tcgaaggacg
     1921 ggcggcaatg aataccaggt ctccgggctc cataccgcct gtttttgcgt ccagttcatc
     1981 aataccggtc atcaacgtcc tggatttctc cagcccctga ttccggcatt caacacgctc
     2041 aaccacttcc ggaagcacat catcaatatg taccggctga atgacgccct ttcctgtcga
     2101 cagtgtgacc atcatgttct gcgcatcctt cagggcatct tcagctgctt cacaggtatg
     2161 cgcatcacgt aatttctgca gcgcctcatt cagtgttttt tctgcatcgc gcagtgcggc
     2221 attgcgccgc aacgctgcaa catagtgctc cagtgaagac ttcacccagg ttttacgccc
     2281 ggtatcagta atcaccgggg caagttccgg catctcatta cacaacagca cggggtcaat
     2341 cactcctgaa acacgggcct gtctgcagat gcctgtatag atatcctgat acgctcgtac
     2401 agaaaaaacg tccgctggca gtgtggccag aatatccatc acttcaggat ctgctccgcg
     2461 cagaaagaac gcgccgatga cagcgccttc aaggtcatcg ttacgccata ctggagttgt
     2521 catgcagcca cacctctgat acgagaacgg taactgggcc agttaaacga caaccagttg
     2581 cgtcccccgt ctgtgatcct gtcggcaatt cggggactga tgaacgccca caactcttcc
     2641 ggtgagaggt tactgataag aatggtgggc aagatacttt cgtacctggc gttgataatt
     2701 tcctgcaaaa tagccatttc agccgcgctg ccaaactgaa cgccaacttc gtcgatgatc
     2761 agcaaatcca ttgacgcata acgctcaata acttcatccg ctgttttttc gctgtcattc
     2821 cgccagcaat ttttcacagc acgggtaagg cgcatcacgt cggtgatctc cacactggcc
     2881 agatagttac ggatgatgtg ttttgccata gccacagcca gatgattttt tccggtacca
     2941 caactgccgg tcataacaag actggtaccg ttctccagca tatctggcca gttctccgca
     3001 tagcggcgac aggccgcaag atttctggct gcgccaggat taacctccag ataattatca
     3061 aactcgcagt cccgaaaacg cagggcaatt ccggcgttat cagtcagctc ttccgccttg
     3121 atggacgaca gctccatggt caaatcgttg gcctcagcga ttaagcagtc agggcagcat
     3181 gaaatttctt ctctgtcctc gccattacga tcgctccaca ccagtatatg cgtgtgatat
     3241 tcgccatgtt tttcgcaata tccgcgacct tcacgcatca ggcaggaacg ataaggccat
     3301 ggcttttcgc ccttctgagc aaatgcaatc tctgcccgta actcatccat tcgcgcctgt
     3361 agtctttttt gttgttcacg caggttaaac gtcatcatcg ctgtcacctc agaatgtcag
     3421 tttgtcactg gatttaccga atttgtcaga catggctccc aggccagcca ggacatcgac
     3481 ctgtcgctgt cgcccacctc tgggagcggc tggctgttgc cagaagtctt cgaagtgacg
     3541 atcgggtcca aagaacgtcg cagcctgctt cacgaactgt gtgccggtat ttcctgtggc
     3601 acgtacccag gcagcatagc gtttcacgcc atcaagcatg gcttcaggtt ttattccctc
     3661 cctgagccgg gctttccagg ctttgaaggc tgcggatttt gagttaccac cagcacgttt
     3721 tggatattcc tgccaggcct gttcaaattc cggtgaatat tcctgtcggg aagaacgcgc
     3781 tggtgcagac gcgtcagcgg atgcgccaat agtgttttta ctctctgtag tattctctga
     3841 agtaatctct gttgtattct ctgtaagatc gaaattggtt ttcccttctc cgcggcgagg
     3901 ggtttcccgt gtccgcggtg aaggctttcc ctcctccgcg aaattgggtt ttacagtttc
     3961 ccgaaaacgg gtttccccat ttcgggaaaa ctgattgttt tcattgataa tttcattaag
     4021 gcgctcacaa tctatacggt agaacatttt gtgctcaaga cgcttgttgg tttcaaccaa
     4081 aatgcctctg gacacaagat gcttacgcgc tacagcctgt tgttcaaatg taagtccggt
     4141 ttcgtgttgt atctcttcac gcgttttatg tacgccttcc gctgcatgtg ctttatcctg
     4201 ccagtaaaaa atctgaccaa agaaaataac agcgtgcgga cttcccatgt atttaacgag
     4261 cccagggtaa taagcaaccg gatgtccaaa atcgagcaga agatcagacg gacgcatagc
     4321 cacctcccag gcgtttaaac atttttccgg gctgaaacgc caccagcgga taactcaggg
     4381 tatgagtacg tccctgaacc tggcagacaa ccttctggct ttcggtactg accaggcaaa
     4441 cccgcagaac gtagccgttg ctggtggtga accactgccc cacacggggg caatggttgt
     4501 atcggtgata caggaaatta acgatgtggc ggatcatggg cgcacctcct tgtcagaacc
     4561 attcagcctg gaatcaacaa gtgcagcacc aaaaacagca tcacctacac ggtcgtacag
     4621 tttgccagcc agcggagatt caacggcctt aagcattggg taaagctggc ttgtccagat
     4681 ttgatggatt tcacgcaaat gcaggtatac gcctctggcg ttttgtgcga cagatgacat
     4741 gtcagccgca ccagctcctg ataagctcct ctccatctgg ttaaaggcat tgatgtatgc
     4801 ctctttgaac cgggcggcac gtttaccagt gaagcccata gcaaggaagg cgaagccgcc
     4861 gcgggtaatt tggtagcagg gaagtttgcg agtaccgccg ttgggctggc gtaccaaaat
     4921 tgatgtctcc gcaaaattgc gggcacaaaa ctctggagaa caatccaaaa tgcggatctt
     4981 tttcagaaca tcgtcatgac gtttagagaa gaagttggca acagccaggg atgaagtaac
     5041 agcctaacca tcaacgatgg caatttcagg ttgagagagg gttgggagag tagtcatggt
     5101 gacagcccct atgttgaatt caaagaactc accacatggg acgccaatca cagaggtggt
     5161 gagacgtaca gggttggcgt aaccggtcaa catagaaccc ggcgcatctt gcgatgtccc
     5221 tgcacgcccc accataattt gggcgtagca atgctcatga cacgaaaaaa ccgcatgagc
     5281 gcggttatgc tctatattga atttcaggac gccaatcccg gcccccgctt tataaggtgc
     5341 agagacagtg taacgtcccg aaattgcaga atcaatatat tgtgcagata taacgatttt
     5401 gttatgtttt gctgtatgaa ctacactatc gaatactaca gtgaagaggt ccgactggag
     5461 gtcgatcagc ttccattgag tatgcgtgcc agataccagc atcttgttga acgcatgaag
     5521 gtatacggca gcaatctcgg agaacctcac accagcgcct ttggtgacgg gcttttcgaa
     5581 ctcagaatta aaggcaacga tgggatcgcg cgtgtttttt actgcactct gacagggaaa
     5641 cgcatcatca tgctgcatag ttttgtaaag aaagcgcaga aaacaccgcc agccgaacgc
     5701 aagaaagctg aaaccagaat gaaggaggtt aagcatgact ggtaaacgca ctctccccac
     5761 catgacacac gacgaaatgg cagccaaatg gatggaagac ccggctttta aagcagaata
     5821 cgacgctatc gctgacgaat tcgcactgct tgatgaaatg ctggcagcac gcaaagaagc
     5881 tggcttaact caggctgaag ttgccgagcg aatgggaaca aaagcgaccg cgatcaccag
     5941 aatggaaagt aatctcgcat caggtatcag cggcccatca tttgccacac taaaaaaatt
     6001 cgcccgcgct actggaaaaa aactccagat ccgcttcgtt taaccacacc gcgccgtcat
     6061 tctggcggcg caccggataa atagtcattt cctcgcacga tgtcttagcc accggatatc
     6121 ccacaggtga gtcgtgtagt tgaaggtttt tacgtcagat tcttttggga ttggcttgcg
     6181 tttatttctg gagcgtttcg ttggaaggta tttgcagttt tcgcagatga tgtcggtgaa
     6241 acttcgtcga tgtcgcctca ttcatacctc ctgtcggtaa atctgacacc ctgaccaata
     6301 gcccaggctg ttgtgtactc gatcagactt gccatgcgct tcacgctcat ctgcgcgctg
     6361 ctttcgcgaa tgttgacgca ttctccttca aggccgggca aaacatcagc ttcctgtttt
     6421 gttgccactg catgaccgct gatcaacaaa accttccatt gttctggttt taaccatttg
     6481 ccgcaccatt gaacctgacg agcgatatcc gccagcatcg cgtgaaattt tgcgttctgg
     6541 tcaagatttc gcttgtagtc agtaatgcgg atggtgactg gcttgtcttt atcgagagga
     6601 gttgagagga tggcatttat tgcggcttgc tgttgttgct tagttcggag gaagattgtt
     6661 tgcttcatcg aaaattcttc tctttaattc cagcggctct gatagctttc attactgcaa
     6721 ttaccgtttt gtccttccca tcctcatgcc ccatcgcata agcaccttct tcaccatctt
     6781 tccaaaagtc gtcattcgat tcgggccagt cgatatccag ttcaatagct gctcgcgatg
     6841 cctgccatat cacccaggca aactctttta attcatcgtc tcctgtgaac tggcttttgt
     6901 cttttgacca cca
//
LOCUS       DAJTQY010000151         6857 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.151, whole genome shotgun sequence.
ACCESSION   DAJTQY010000151 DAJTQY010000000
VERSION     DAJTQY010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6857
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.151"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(54..533)
                     /gene="xdhC"
                     /locus_tag="OO349_003680"
     CDS             complement(54..533)
                     /gene="xdhC"
                     /locus_tag="OO349_003680"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HCR5868602.1"
                     /translation="MNHSETITTECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGTVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(530..1408)
                     /gene="xdhB"
                     /locus_tag="OO349_003681"
     CDS             complement(530..1408)
                     /gene="xdhB"
                     /locus_tag="OO349_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HCR5868603.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAATFIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(1419..3716)
                     /gene="xdhA"
                     /locus_tag="OO349_003682"
     CDS             complement(1419..3716)
                     /gene="xdhA"
                     /locus_tag="OO349_003682"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HCR5868604.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALTEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRICSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIDWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            4131..4886
                     /locus_tag="OO349_003683"
     CDS             4131..4886
                     /locus_tag="OO349_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HCR5868605.1"
                     /translation="MSAGRLNKKSLGIVMFLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            4965..5038
                     /locus_tag="OO349_003684"
     tRNA            4965..5038
                     /locus_tag="OO349_003684"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4997..4999,aa:Gly,seq:ccc)
     gene            5153..6757
                     /locus_tag="OO349_003685"
     CDS             5153..6757
                     /locus_tag="OO349_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR5868606.1"
                     /translation="MIQLSSSHKLPAVYYLYQRNGVYYFRLRVRQSNNDRMTSVSLRT
                     KDRRTAMAHSRHIKAALKAIHADRPNATYEEMREHLKDIAECELSMGRSDLFEPDMRD
                     IYRDQYGELGESLTDALASEPLSIDQHRYINEALKVLKACMRRIEAGDSQPLIDYVDR
                     FNDIDRQDDQADSVSLSVSAPEVKPEVTPSITIASLFEQYEAENSQNWKPATLRENKA
                     SHAALIEIFDHLGLNADANRADMLKVRDVLQQLPKNRKQRFKDAPLADLLSREDKTDC
                     LDVVTINNKYLIKMAAVFRWAVRNDLIKKNMTEGLELKVPQRKASGARNAFSTEQVGQ
                     LLVAAKAYSQKTSGKPYHYYVTALAAITGARLNEIAQLQVKDVRTTEAGTVYIHINED
                     DSSLPGKSIKNAHSDRCVPLVDGAYGFILADFMALVETRRGADGDDAMVFDGLRLMKN
                     GYGEQVSKWFNRTLLPKVLVDRSGLAFHSFRHTVATQLKQHGVELAYAQAIMGHSSGS
                     ITYDRYAKEVEVDRLVNVMADVYKET"
BASE COUNT         1708 a         1686 c         1788 g         1675 t
ORIGIN      
        1 tgttaaacac ataacctcag tgaatccggc ctgaattcag gccggatatc cttttacttc
       61 gttttctcgc aatccagaac tgtatttaca atcatctgat accccgtgca gcgacaaaga
      121 tttcccgcca gtccgcgacg aatttccgta atggttaatg gcttctcgcg tggtttcgcc
      181 agcattgccg tggtagccat aatcaggcca ggcgtacaaa acccgcactg cactgtgccg
      241 gatttcgcat aagcctgctg aacatgagaa agttttccgc ctttcgcttc accttccagc
      301 gtgcggatct cttttccttc agcccaggcg gcaaggtata agcaactgtc tattgctgtg
      361 ccgtcgacca acaccgtaca ggcaccacat tcacccacgc agcacccttg tttgacactt
      421 agcagtcctt gttcgcggag taattccgag agcggcgtgc ctggtgcggc gtgaagctga
      481 aaaggcatcc cgttaatggt gcattcggtg gtaattgttt cgctgtgatt cattgcaatt
      541 ttccccccgc cgcggcgacg gcttcgctaa tcactttttt ggtcatcgtc tggatgagat
      601 gcagacgaaa ctctttactg gcccgccatg aagaacgcgg ggcgacatct tgcaggacag
      661 attcgctgat agcttccagc gtttgcaggt ttaatggcgc attttgtgca gtctgttcgg
      721 catgttggca gcgaatcggc gttggcgcgg caacaccaaa tgccaggcgt aattcgctga
      781 aattgccgtt atccagtcgg caatgtgcgg cgcagccaat cgttgaaata tccattgcgt
      841 cgcgcatggc atatttaaaa tgcgcgctgc ccgcgtgttc tttcggctgt ggcggaaaat
      901 gaaaggcgac gaggatttcg tcatgctcaa gagacacttt gcccggcccg gtgtgaaagc
      961 cattaatcgg gacgaaacga acaccgcgcg gggagtggat ctccagtttc gcgtcataaa
     1021 ttagcgttgg cgtggcagaa tctgcgctgg tggcaccgtt gcaaatattt ccaccgtagg
     1081 tagcgacgtt acggatctgc ggtccagcaa tgaacgtggc cgcagcacat aacgccggga
     1141 gatgacgttg agttatagga tcttctatta gctgggtaaa tgtcgttgca gagccgatac
     1201 gtagcgagcc atcttccgcc agcgtaattc cccgcagctc cgccagatta tgaatatcaa
     1261 caatatggcg ataacggtca ttgtgatggt ggagctgaat cagtacgtca gtgccaccgg
     1321 cgagcagttt ggcctgcggg ttgtcagcca gcaggttgat ggcatcggca agggttgctg
     1381 cgcgatggta agaagcaaaa tcaaacatga tgttatcctc aaatcaatcc tgccagatgg
     1441 aactcttcat ataaccgttt tggcgtcagc ggcagtgtat tgattgcaac accggtagcc
     1501 atcttcaccg cgttacgaat agcagcggca acaggaatta tgggtggctc acccagtgac
     1561 ttatgtccgt atgcggattg cggctcattg atttcgacga acgcgctttc cagttgtggc
     1621 agatccggca tggtcggcat tttgtaatcc agcagattgg ggttacggac cacgccgctt
     1681 ttagcatcga tgatcatctc ttcaaatagc gcccagtcaa tgcccattcc cattccgccg
     1741 tgtacctgac cttctgccag cagtggatta agaatatgcc ctgaatcatg aacgttgagg
     1801 atgcggttga tggtgacttt gcacagcgca atatcgaccg tcagatcaac aaaggtacag
     1861 ccaaacgccg gtgggttagt ggtggttttg atggagcttt cagcagagag ctgcccgccg
     1921 cgttcagggt ggtagaaagc gtccatcgcc aaatctttta acgacattaa cggctcttcc
     1981 ggtcgttcaa ccagcacgat atggcctttt atcagggtca aattcatcgc tgactgatgt
     2041 agcatgactg cggcgtgagc gatgattttc tcttttaata atagtgccgc actgcgcagc
     2101 gcaggcgcgg caacatagct ctggcgtgag gcaaatgcgc cgggatcgaa cggcgtaacg
     2161 tcggtatctt gtgttgaaat aacgcgaacg tcgctgaccg gaacccccac ggtttctgcc
     2221 accatttgcg agaagacggt gtcggcaccc tgaccgattt ccgtcgcgcc gctttgcacg
     2281 ttgatggttc catcctgatt catcagaagg cgcgcgcctg ctatttctac gccgacaggc
     2341 caggtgttag aggtgtagct aaaacaggcg acgccaacgc cgcggcgcaa attgccttgc
     2401 tggttctggc attctgcacg gcgtttttcc cattcaaaga ttttccggcc tttttcaaga
     2461 cactccggca accctgcgct gcaaatacgt ttgcccgtga gcggattagc atctccttcg
     2521 cgggcggcgt tgcgtaaacg aatttcaaca ggatcaatac ctaacgctgt cgcggcgtca
     2581 tcaagcatag actcaacggc aaatacgact tgtggcgcgc cataaccacg catcgcacca
     2641 gccgagggga ggttggtata gcaggtcttt gaactgtaag cgtaggcaca acgaggataa
     2701 aggtaagcga ctttattccc ccccgcagaa gcgatggagt gcccgtgaga tgcataagcg
     2761 ccggtgttag acagaacatc cagactataa cctttcaagg ttccgtcgcg gttcacgccc
     2821 atttgcccgt caatagtaaa agcgtggcgg gtacgggttg cgaggaaaca ctcttcacgg
     2881 ctaagggaaa ctttcaccgg aatgccgcca agcttgctgg tcaggaatgc cgccattggc
     2941 tcttccagta catcctgttt attaccaaaa ccgccaccga caaacggttt gatgactcgt
     3001 acgcatgacc agggaatatc cagcgcctga ccaaccacgc ggcgaacaat gtgcgggatc
     3061 tgggtgctgg aaacgatggt aattcgcgag tcatcctcca tccacgccag cgatgttacg
     3121 ctttccatgt gacaatgttg aataacggga gtctgatagt gcccctgtac ctggtagtcg
     3181 gcggcatcga ttgtttgttg gacattgccc gtcgacatcg tgctttgttt cagtaaattg
     3241 ccgccgttat ggattggtgc agcgtcttct gtcagcgccg cttctggcgt ggtgataacg
     3301 ggtaattctt gccactcaat gctgaccaat tgcgccgctt tttctgccgt gagttcatcg
     3361 cgggccacga cgatggcaac ggcgtcacca tgatgacgaa catggcgagt tagcagtgcg
     3421 cgatcggcgg tatcgcgctt gttttcgtca agtgtccagg catgcccagc tgtagcgaat
     3481 ggaatatcag gcacatcctc ccaagtaaaa atcgccagta cgcccggtaa acttctggct
     3541 tgttcatcat taatacttac ggcataacca tgtgcgatag ggctacgtac atatttcgcg
     3601 taacacatgc ccgccataac ataatcgtca gtatatcgtg cccgcccggt gaccttagca
     3661 atggcatcga cgcgcatgca tgattcaccc gtagcggttg cttcccgcgc ttccatagaa
     3721 atcccctcga tttgaacatt tggttcttat ggcaaagatt tagtctaagc aggcaagatt
     3781 tacgccagaa acgacgataa gcgttagggg aggaaggaag tgtgatgacg ttaaaaaaac
     3841 acagatatga tacgaaacag gactctgaat agggacagtg atacgtatca atatgagtgg
     3901 cgataaaatc aatgtgataa atcaggatta ttacgcatca ggatgggaaa tagggtaaaa
     3961 agtcaggaac ttcgatacct ctacagcgaa tattttgctg atgatacttc cctttgccaa
     4021 atgaaattaa cctctttctc tattaccacg tttttccaga agcaagagat tgggtatcaa
     4081 gaggctggct gctatgatat ggcgtcttgt tttttaagcg aggaaagatt ttgagtgcgg
     4141 gacgcctgaa taaaaaatct ctgggtatcg tgatgttttt atcggttgga ctgctattgg
     4201 cgggctgttc gggtagcaaa tcatccgata caggaacata ttccggctcc gtttacaccg
     4261 tgaaacgggg ggatacgcta tatcgtattt cgcgcaccac gggaaccagc gtaaaagagc
     4321 tggcgcgact gaacggcatt tccccccctt acaccattga agttggtcag aaactaaaac
     4381 tgggtggggc gaaaagtagc agtagtacac gtaaatcaac cgccaaatca acgactaaaa
     4441 ccgcatcggt tacaccgtca tcagcggtac cgaaatcttc ctggccgcca gtagggcaac
     4501 gttgttggtt atggccaacg acagggaaag ttatcatgcc gtattcgaca gcagatggcg
     4561 gcaataaagg gattgatatc tcagctccac ggggtacacc tatttacgcc gcgggtgcag
     4621 gaaaggtggt gtatgtgggc aaccagctgc gtggctacgg taatctcatc atgattaaac
     4681 acagtgaaga ttacattacg gcttacgccc ataatgacac gatgctggta aataatgggc
     4741 aaagcgtgaa ggctgggcaa aaaatcgcca ctatggggag cacggatgcg gcatctgttc
     4801 gcctgcattt ccagattcgt taccgtgcaa cggcaattga tccgctacgt tacttgccgc
     4861 cgcagggcag caagccaaaa tgctgatggc gaattaatca gcagtcagca gcgcggcact
     4921 tgctaaggag agcgtaaggt ttataatgcc ttacgcatct cgaagcgggc gtagttcaat
     4981 ggtagaacga gagcttccca agctctatac gagggttcga ttcccttcgt ccgctccatg
     5041 acctttctta aagtactcca acctactgaa tcctaaggga tatggcttta agcctccttg
     5101 cagagttact tactgtaacc cactgtgtaa ccctctcagg gcatacagac atatgattca
     5161 gctatccagt agtcacaaac ttccagcagt ctactatctc taccagcgta acggcgtcta
     5221 ctacttccgt cttcgggtca gacaatcaaa caatgataga atgacctcag tatcattacg
     5281 gaccaaggat agacgcaccg ctatggctca ctcaaggcac atcaaggcag cactcaaggc
     5341 aatacacgca gacagaccca acgctaccta tgaggagatg agggagcacc ttaaggacat
     5401 cgctgagtgt gaactgtcta tgggccgttc agatctcttt gagcctgaca tgagggatat
     5461 ctaccgggac caatacggag aacttggtga gagccttacc gatgctctgg ctagtgagcc
     5521 gctaagtatt gaccagcatc gttacatcaa tgaggccctg aaggtgctaa aagcctgtat
     5581 gaggcgtatc gaagcgggag atagtcagcc tctgatagat tacgtcgatc gttttaatga
     5641 tattgatcgg caagatgacc aagcagactc tgtgtcccta tctgttagtg ctccagaagt
     5701 aaaaccagaa gttacgccta gcattaccat agcgagcctc tttgagcaat acgaagcgga
     5761 gaactctcag aactggaagc ctgcaacctt aagggaaaac aaagcgtcac acgctgcgtt
     5821 aatcgaaata ttcgaccatc tgggtctcaa tgcagacgct aacagggctg acatgcttaa
     5881 ggtccgtgat gtgctccagc agttacctaa gaaccgtaag cagcgcttta aggatgcccc
     5941 acttgctgac ctgttgagcc gtgaggataa gacagattgt ctggatgtcg ttaccatcaa
     6001 caacaagtat cttattaaga tggctgctgt ctttaggtgg gctgtgcgca atgacttgat
     6061 taaaaagaac atgactgagg gtctggagct taaagtccct cagcgtaaag cctcaggggc
     6121 ccgtaacgcc ttctctacgg agcaggtggg gcaactactg gtcgcagcta aggcatactc
     6181 tcagaagacc tctggtaagc cctaccacta ttacgttacg gctttggctg ctatcactgg
     6241 ggcgagactc aatgaaatag cccaactaca ggtcaaagac gtcaggacca ctgaggcggg
     6301 aaccgtttat attcatatca atgaagacga tagtagtcta cccggtaaga gcatcaagaa
     6361 cgctcatagt gaccgctgtg tgcctctggt cgatggggcc tatggtttta tattggctga
     6421 ctttatggcg cttgtagaga cccgtagagg cgctgacggt gatgacgcta tggtgttcga
     6481 tggtctgcgt ctcatgaaga acggttacgg tgagcaggtg agtaaatggt ttaatcggac
     6541 gttgttgcct aaagtcctcg tagaccgtag cggcttagcg tttcactcgt ttagacatac
     6601 agttgcgacc cagttaaaac aacatggggt cgagttggcg tatgcacagg cgatcatggg
     6661 gcatagttca gggtctatta cgtacgaccg ttatgctaaa gaggtcgagg ttgataggct
     6721 ggtcaatgtg atggctgatg tttataagga gacctagagg agaccatagt ctaggatgac
     6781 acttactaca gacctgtgtg gtcatgtttt tgactcatgg ttttctctac ttgtcaaggg
     6841 ataattaata cgactca
//
LOCUS       DAJTQY010000152         6619 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.152, whole genome shotgun sequence.
ACCESSION   DAJTQY010000152 DAJTQY010000000
VERSION     DAJTQY010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6619
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.152"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            64..2496
                     /locus_tag="OO349_003686"
     CDS             64..2496
                     /locus_tag="OO349_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HCR5868607.1"
                     /translation="MAELNLSNLTEADIITKCVMPAILNAGWDNTTQIRQEVKLRDGK
                     VIVRGKVAARRTVKSADIVLYHKPGIPLAVIEAKANKHEIGKGMQQGIEYARLLDVPF
                     VFATNGDGFIFRDATAAEGECLEKQITLDDFPSPAELWQKFCLWKGYTQAQLPVITQD
                     YYDDGSGKSPRYYQLQAINKTIEAVSNGQNRVLLVMATGTGKTYTAFQIIWRLWKSKN
                     KKRILFLADRNILVDQTKNNDFQPFGTAMTKVSGRTIDPAYEIHLALYQAITGPEEDQ
                     KAFKQVAPDFFDLIVIDECHRGSASEDSAWREILDYFSSATQIGLTATPKETHEVSST
                     DYFGDPVYVYSLKEGIEDGFLAPYKVVRVDIDVDLQGWRPTKGQTDLNGEVIDDRIYN
                     QKDFDRTMVIDERTELVARTITDYLKRTNPMDKTIVFCNDIDHAERMRRALVNLNPQQ
                     VKKNDKYVMKITGDDEIGKAQLDNFINPKKPYPVIATTSELMTTGVDAKTCKLVVLDQ
                     NIQSMTKFKQIIGRGTRIDERYGKLWFTILDFKKATELFADERFDGIPEKVMDTTPED
                     IADPESDFEEKLEEISEHDDEQVTGVDEPPAPPYQVTDTDDVGPFPEEDEKKIRKFHV
                     NGVAVGVIAQRVQYYDADGKLVTESFKDYTRKTLLKEYASLDDFTRKWQDADRKEAII
                     HELEQQGIIWEVLAEEVGKDLDPFDMLCHVVYGQPPLTRKERAENVHKRNYFTKYSEA
                     AQAVLDNLLDKYADAGVQEIESIQVLKLKPFDSMGTLPEIIKTGFGDRNGYNQALSEL
                     ENEIYQLPPRSA"
     gene            2563..4032
                     /locus_tag="OO349_003687"
     CDS             2563..4032
                     /locus_tag="OO349_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HCR5868608.1"
                     /translation="MSISSVIKSLQDIMRKDAGVDGDAQRLGQLSWLLFLKIFDAQEE
                     ALELEQDNYQYPIPQRYLWRSWAANAQGITGDSLLEFVNDDLFPALKNLTAPIDKNPR
                     GYVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIYEQILKDLQSAGN
                     AGEFYTPRAVTRFMVDRVDPKLGESIMDPACGTGGFLACAFDHVKNKYVKSVADHQTL
                     QQQIHGVEKKQLPHLLATTNMLLHGIEVPVQIRHDNTLNKPLSSWDEQLDVIVTNPPF
                     GGTEEDGIEKNFPAEMQTRETADLFLQLIVEVLAKNGRAAVVLPDGTLFGEGVKTKIK
                     KLLTEECNLHTIVRLPNGVFNPYTGIKTNLLFFTKGQPTKEIWFYEHPYPAGVKNYSK
                     TKPMKFEEFQAEIDWWGNEADGFASRVENEQAWKVSIDDVIARNFNLDIKNPHQAETV
                     SHDPDELLAQYAKQQAEIQTLRNQLRDILGAALSGKEVN"
     gene            4032..5693
                     /locus_tag="OO349_003688"
     CDS             4032..5693
                     /locus_tag="OO349_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCR5868609.1"
                     /translation="MSVEKLIVDHMETWTSALQTRSTAGRGSSGKIDLYGIKKLRELI
                     LELAVRGKLVPQDPNDEPASDLLKRIAAEKAELVKQGKIKKQKPLPEISEEEKPFELP
                     EGWEWVHLPDIYCSISESSRKIKSSEILPEGKYPVIEQSQEFISGYCNNECLLIKLNN
                     PVIVFGDHTRNIKFIDFDFVVGADGVKILSPILICERFFFWQLRSFKLDVRGYARHFK
                     VLNSCLFALPPIAEQERIVEKVSSLMSLCDQLEQQSLTSLDAHQQLVETLLGTLTDSQ
                     NTAELAENWARISEHFDTLFTTEASVDALKQTILQLAVMGKLVPQDPNDEPASELLKR
                     IAQEKAQLVKEGKIKKQKPLPPISDEEKPFELPEGWEWCRIGNIVNIKSELVSPKDYL
                     NLYQVAPDIIEKGTGRVISKRTVKESGVKGPNSRFYKGQIVYSKIRPSLSKVFLAEYN
                     GLCSADMYPLDCYINPNYLLKYILSIPFLMQVKKAENRIKMPKLNSDSFYNIIVAIPP
                     YNEQQAIFDKINSIEAVCNGLISYIGIYHKTQLHLADALTDAAIN"
     gene            5914..6255
                     /gene="symE"
                     /locus_tag="OO349_003689"
     CDS             5914..6255
                     /gene="symE"
                     /locus_tag="OO349_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HCR5868610.1"
                     /translation="MTDTHSIAQPLEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTAVDVKVMEGCIVLTAQPAAAEESELMQSLRKVCKLSARKQRQVQEF
                     IGVITGKQKVA"
     gene            complement(6302..>6619)
                     /locus_tag="OO349_003690"
     CDS             complement(6302..>6619)
                     /locus_tag="OO349_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5868611.1"
                     /translation="LFDHLRDEVMRLDAGITQEVLKLYIAFKAETNFVDVVPQKSRLR
                     LSLNMQFHELVDPKGIAKDVTNVGRWGNGDVEIGFSDLAQLPYIMGLIRQAFEKQMES
                     ALV"
BASE COUNT         1950 a         1581 c         1526 g         1562 t
ORIGIN      
        1 tcatagccag gctattgatt ttatgtacaa tcagaatcgt cctgataaaa gcgagaaccc
       61 cccatggcgg aactgaacct aagtaacctg acggaagcag acatcattac caaatgcgtt
      121 atgccagcca ttctcaatgc aggctgggac aacacaacgc aaatcagaca ggaggtcaaa
      181 ctccgggacg gtaaagtcat cgtacgtggt aaagttgcgg cacgcagaac ggtaaaatct
      241 gctgacatcg tgctgtacca caagccaggc attcccttag ccgtgattga agcaaaagcc
      301 aacaaacatg aaattggcaa agggatgcaa cagggcattg aatatgcgcg cctgctggac
      361 gttccttttg tttttgccac caacggtgat ggctttatct tccgcgatgc caccgcagcc
      421 gaaggtgaat gccttgaaaa gcaaataacg ctggatgact tcccctcccc cgctgaactc
      481 tggcaaaaat tctgcctctg gaaaggatac acacaagctc agcttccggt gattactcag
      541 gactattacg acgatggcag cggcaaatcg cctcgttatt atcaacttca ggcaatcaac
      601 aaaaccattg aagccgtctc caacgggcaa aaccgcgttc tgctggtcat ggcgaccgga
      661 acagggaaaa cctataccgc attccagatc atctggcgcc tgtggaaatc aaaaaataaa
      721 aaacgcattt tgttccttgc cgatcgcaac attctggtcg accaaaccaa aaataatgat
      781 ttccagccat ttggtacggc aatgaccaaa gtcagcggac gcaccattga tcccgcttat
      841 gaaattcacc tcgcgctcta tcaggctata actggcccgg aggaagacca aaaagcgttt
      901 aaacaagtcg caccagattt cttcgatctg atcgtgatcg acgaatgcca tcgcggcagc
      961 gcatccgaag acagcgcctg gcgagagatc cttgattatt tcagttccgc cacccaaatt
     1021 ggcttaaccg ccacgccaaa agagacgcat gaagtctcca gcacggatta cttcggcgat
     1081 ccggtttacg tctactcact aaaagaaggg atcgaagacg gcttcctcgc tccttataaa
     1141 gttgtccgtg ttgatattga tgtcgacctg caaggctggc gcccaaccaa agggcaaact
     1201 gacttaaacg gcgaagtgat tgacgatcgt atctataacc agaaagattt cgatcgcacg
     1261 atggtgatcg acgaacgcac agaactggtt gccagaacca ttaccgacta cctcaagcgc
     1321 accaatccga tggataaaac catcgtcttc tgtaacgaca tcgatcatgc agaacgtatg
     1381 cgccgcgccc tggttaatct caacccgcag caggtgaaaa agaacgacaa atacgtcatg
     1441 aaaatcaccg gcgatgatga aattggcaaa gctcagttgg ataacttcat caacccgaaa
     1501 aaaccgtacc cggttatcgc gaccacttca gagctgatga ccactggtgt ggatgctaaa
     1561 acctgcaaac tggtagtact agaccagaac atccagtcga tgaccaaatt caagcaaatt
     1621 atcggtcgtg gtacacgcat cgacgaacgt tacggcaaac tctggtttac catcctcgac
     1681 tttaaaaaag ccaccgaact gtttgccgat gagcgtttcg atggcattcc cgaaaaagtc
     1741 atggatacca caccagagga tatcgccgat ccagaatctg attttgaaga gaaactcgaa
     1801 gaaatcagcg aacatgacga cgaacaggta acaggcgttg atgaaccgcc tgcgccacca
     1861 taccaggtta ccgataccga tgatgtcggc ccatttccgg aagaagacga gaagaaaatc
     1921 cgcaagtttc acgtcaacgg tgtagcggtg ggcgttattg cacagcgcgt tcagtattac
     1981 gacgccgacg gtaaactggt taccgaatcc tttaaagatt acacccgcaa aacactgctc
     2041 aaagaatatg cctcgctgga tgactttacc cgcaagtggc aggacgccga tcgcaaagaa
     2101 gcgatcattc acgagctgga gcaacagggg atcatctggg aagtactggc agaagaagtc
     2161 ggtaaagatc tcgacccgtt cgacatgctt tgccacgtag tgtatggtca gccgccgtta
     2221 acccgcaaag agcgcgccga gaacgtgcac aagcggaact acttcacaaa atactctgaa
     2281 gcagcgcaag ccgtgctcga taatctgctg gataaatacg ccgatgcggg cgtacaggag
     2341 atcgaaagta ttcaggtgct gaaacttaaa ccattcgaca gcatgggcac cttaccggag
     2401 attattaaaa ccggatttgg cgaccgtaac gggtataatc aggcgctcag cgagctggaa
     2461 aacgaaatct accaattacc gccccgctct gcttaacaac tctcttttgc gccttctgca
     2521 accatcatgt tgcagaaggc cttgtttttc atggaataca acatgtctat cagctcagta
     2581 atcaaatcat tacaagatat tatgcgcaaa gatgccggtg tggacggcga tgcgcagcgt
     2641 ctcggtcagc tctcctggct gctgtttttg aaaatcttcg atgcccagga agaggcgctg
     2701 gaactggagc aggataacta tcaatatccg atcccacagc gttatttgtg gcgcagttgg
     2761 gccgccaacg cgcagggcat taccggtgat tccctgctgg aattcgttaa tgatgatctg
     2821 tttccggcgt tgaaaaacct cactgcgcct atcgataaaa acccacgcgg ctacgtggtg
     2881 aagcaggcgt tcagcgatgc ctataactat atgaaaaacg gtacgctact gcgccaggtg
     2941 atcaacaagc tgaacgaaat tgactttacc agcgccagcg aacgccatct gtttggtgat
     3001 atttacgaac agatccttaa agatctgcaa tctgcgggca atgcgggcga attctatact
     3061 ccacgcgccg tcactcgctt tatggtggat cgcgttgatc cgaaactcgg cgaatccatt
     3121 atggacccgg cctgcggtac gggcggtttt cttgcctgcg cgttcgatca tgtaaagaac
     3181 aaatacgtga agagcgtcgc cgatcatcag acactgcaac aacagatcca cggtgttgag
     3241 aaaaaacagc ttccgcacct gctggcgacc accaatatgc tgctgcacgg cattgaagta
     3301 ccggtacaaa ttcgtcacga caacaccctg aataaaccgc tttcctcctg ggatgagcaa
     3361 ctggatgtca ttgttaccaa cccgccgttt ggtggcacgg aagaagacgg tattgagaag
     3421 aactttccgg cagagatgca aacccgcgaa acggcggatc tgtttctgca actgattgtg
     3481 gaagtactgg cgaaaaacgg tcgtgcggcg gtggtattgc cggatggcac actatttggc
     3541 gaaggtgtta aaaccaaaat caaaaagcta cttaccgaag agtgcaacct gcataccatc
     3601 gtgcgtttac cgaatggtgt gtttaatccc tataccggca ttaaaaccaa cctgttgttc
     3661 tttaccaaag gtcagccaac caaagagatt tggttctatg aacatccgta tccggcgggc
     3721 gtgaaaaact acagcaaaac caagccgatg aagtttgaag agtttcaggc ggagatcgac
     3781 tggtggggta acgaggccga tggttttgcc agccgcgtcg agaatgagca agcgtggaaa
     3841 gtcagcattg atgacgtgat tgcgcgcaac ttcaatctgg atattaaaaa cccacatcag
     3901 gcggaaaccg tcagccatga tccggacgaa ctgttagcgc agtatgcaaa acagcaggcg
     3961 gagatccaga cgctgcgtaa tcaactgcgc gatattcttg gcgctgcgct gtctggcaag
     4021 gaggttaact aatgagtgtg gaaaagctga tcgttgatca tatggaaacc tggacctcgg
     4081 cgttgcaaac ccgttccacc gccgggcgcg gcagctccgg taagattgat ttgtatggta
     4141 ttaagaaatt gcgtgagctg attctggaac tggcggtgcg cggtaaactg gtgccgcagg
     4201 atccgaatga tgaaccggcg tcggacctgc tgaagcgtat tgcggcggaa aaagcagagc
     4261 tggtgaagca aggcaaaatt aaaaagcaaa aaccactgcc agaaattagt gaggaagaga
     4321 agccgtttga attgccggag ggatgggagt gggtgcacct ccctgatatt tattgctcaa
     4381 tttctgagtc atctagaaaa ataaaatcct ccgaaatact acctgaggga aaatatcctg
     4441 ttatcgagca aagccaagaa tttatttctg gttattgcaa caatgaatgt ctgttaataa
     4501 agttaaataa tccagttata gtttttggtg atcacacaag gaatattaaa tttattgatt
     4561 ttgattttgt tgtcggcgct gacggtgtga agattttatc acctatatta atttgtgagc
     4621 gtttcttttt ttggcagtta cgatcattta aattagatgt aagaggatac gcgagacatt
     4681 ttaaagtatt aaatagctgc ttatttgcat taccccccat tgcagaacaa gagcgaatag
     4741 ttgaaaaagt tagctctttg atgtccctct gcgaccaact agaacagcaa tccctgacca
     4801 gtctggacgc acatcagcaa ctggttgaaa ccctgttggg aacgcttaca gacagccaaa
     4861 acaccgcgga actggctgaa aactgggcgc gtattagcga gcatttcgac accctattta
     4921 ccaccgaagc cagcgtggat gcgttaaaac agaccattct gcaactggcc gtaatgggca
     4981 aacttgtgcc gcaggatccg aatgacgaac ccgcctctga actgctcaaa cgtattgcgc
     5041 aggaaaaagc tcaattggtg aaagaaggga aaataaaaaa acaaaaacca ctgccgccaa
     5101 ttagcgatga ggaaaaaccg tttgaacttc cggaagggtg ggaatggtgt cgtattggta
     5161 atatagttaa tattaaatct gaattagtat caccaaaaga ttatttaaat ttatatcaag
     5221 tagcacctga tattattgaa aagggcactg ggagagtgat atcaaagaga acagtgaaag
     5281 aatcaggagt caaagggcca aatagtcgct tttataaagg acaaattgtt tattctaaaa
     5341 tccgcccgtc attatccaaa gtttttttag cagagtataa tggtctttgc agtgctgata
     5401 tgtatccatt agactgttac attaatccaa attacttatt gaaatatatc ctttccattc
     5461 catttcttat gcaagtcaaa aaagcagaga acagaataaa aatgccaaaa cttaattctg
     5521 acagttttta caatattatt gttgcgattc ccccctataa tgaacaacaa gcaatatttg
     5581 ataagataaa ttctattgaa gcagtatgca atggtcttat ttcttatatt gggatttatc
     5641 ataaaaccca actgcatctt gcagacgcac tcactgacgc agcgataaac taattccctc
     5701 gccctcacaa aaccaataaa gcggctaaat agccgcttta ttcacatcac caaaatttat
     5761 atttcacgct aattttcatc ttattttcat taatttttag ctggactttc cctgtattta
     5821 ctgatgatgt atccaggtat ttagcacggt gcggatgtgc gccaacacac tcgcaccgct
     5881 aatcacaatc actatttata gagaatagta atcatgactg acacgcattc tattgcacaa
     5941 ccgttagaag cagaagtctc cccggcaaat aatcgtcaat taaccgtgag ttatgcgagt
     6001 cgttacccgg attacagccg tattcccgcc atcaccctga aaggtcagtg gctggaagcc
     6061 gccggttttg ccaccggcac ggcggtagat gtcaaagtga tggaaggctg tattgtcctc
     6121 accgcccaac ccgccgccgc cgaagagagc gaactgatgc agtcactacg caaggtgtgt
     6181 aagctgtcgg cgcgtaaaca aaggcaggta caggaattta ttggggtaat tacaggtaaa
     6241 cagaaagtcg cctgatacat tgacccggta gcgccagtgc taccgggtat tattcgccgc
     6301 gttataccaa cgcgctctcc atctgttttt caaatgcctg acgaattaat cccataatgt
     6361 aaggaagttg tgcgaggtcg ctgaaaccaa tttctacatc gccattaccc cagcgcccaa
     6421 catttgtcac atctttggca atacctttcg gatcgaccag ttcatgaaac tgcatattaa
     6481 gcgacaatcg cagtcggctt ttttgcggca caacatcaac aaaattcgtt tccgctttaa
     6541 acgcaatata tagcttcaat acttcctgcg tgatccctgc gtccaggcgc ataacttcat
     6601 cgcgaagatg atcgaataa
//
LOCUS       DAJTQY010000153         6581 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.153, whole genome shotgun sequence.
ACCESSION   DAJTQY010000153 DAJTQY010000000
VERSION     DAJTQY010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6581
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.153"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(97..2100)
                     /locus_tag="OO349_003691"
     CDS             complement(97..2100)
                     /locus_tag="OO349_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5868612.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     SALIATFSDGVRTQLANGQALKEAQCTYGASGMCRHRVMLVLSYQRLCATAQPTGKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKSITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLSGISQPLIHYEAAFSRAQQAAERCSWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARSDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQEDSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASASAPYAIERMAALLQQTDDPVCLVSGFVSFVDGQLTLEPQVMMTKTRAWALDAET
                     APVVVSLSSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAINQAELLANDLTA
                     VGFYRLAHVLAQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            complement(2097..3233)
                     /locus_tag="OO349_003692"
     CDS             complement(2097..3233)
                     /locus_tag="OO349_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCR5868613.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSSGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIEFPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAMEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALINVGAQIAAMTPGELASWLAENLQS"
     gene            complement(3226..5505)
                     /locus_tag="OO349_003693"
     CDS             complement(3226..5505)
                     /locus_tag="OO349_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5868614.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQADRRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQEESQTLLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFA
                     QLRGDSPGDALNRQREAFMARWIGWAMQQNNGDVLVVCGGWHAPALAKMWRECPQVIN
                     TPELPSLADAVTGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWQWGLQQAGEQLLKT
                     VLTRLRQHKLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFNPNGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEERWKLTRPLSQHAAL
                     IEAACFGATLQEAARHKLEADMLDAGGIGSITTCLSQAASAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVQLPGVSLSAAVALLERHSQAIHVLALDRGATLGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPAFIAGFSSHLNQLS
                     DADFTNALPDLRAAMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALASLQHWGVFHV"
     gene            complement(5516..>6581)
                     /locus_tag="OO349_003694"
     CDS             complement(5516..>6581)
                     /locus_tag="OO349_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HCR5868615.1"
                     /translation="QRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAARAFILGDSA
                     QNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATSISGDAGLTIQG
                     GASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITRTPLEVQDC
                     LLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKRRFDFETVF
                     PIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEKKTSMDTLT
                     AIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRARLRRYFEQR
                     VATHKEAHWQAYYQARHRLP"
BASE COUNT         1502 a         1816 c         1771 g         1492 t
ORIGIN      
        1 actggtgact ctcaccaggg gctatatagg atatgcctaa taccgtggcg tgagcagtcc
       61 ggaactggag tagaactctt agtaaaaagc actatttcat ccttgttgct gaagcatggg
      121 gaataattgt tcgcaaagca aaacaccgtt attcattgct tctacccgtg cctcgctttc
      181 tgtattacga aattgtgcca acacatgtgc cagccgataa aaacccaccg cggtgaggtc
      241 attcgccagc aactctgcct gattaatagc gctctgttcc tgatagcgcc agccgttatg
      301 gagcagttga ataagtaacg cctggcagcg catcagcaac tgatgagcag tagacggcac
      361 aggcagaacg ctggcagaag atagtgatac caccacaggc gcagtttctg cgtccagcgc
      421 ccaggcacga gtctttgtca tcattacctg tggttccagt gtcaattgcc catcaacaaa
      481 actgacaaag ccagaaacca gacacacggg gtcgtctgtt tgttgcaaaa gcgccgccat
      541 gcgttcaatg gcataaggcg cgctggctga ggctggcagt gataacgtca gcagattatc
      601 ttcaccttcg ccgctgatta cctgcgcatc cagcgtctgg cggctgctat cccaaccgag
      661 cgaaatacac tcagcgaccg gcagaataaa taagttatcg acctgattaa gaggccgtat
      721 gcaggcgggg ggacgctggc gtaaatattc ccgcaaagcc acaatgcccg gctggcgtaa
      781 cggcgcgctc aacatttgcc aggcatcagg cgacagcggt acaacgctgc ttaagcggtt
      841 gcgggtagct aacagcagct cgccatcggc actgcgtttt gctgcttgtg aaacaatttg
      901 cccacccgcc agtgcgccag cctgaaaact aaacagccga cgcgtagctg ccggtgagtc
      961 ttcctgttca cttcgcggcc aactgcgcga aaggtgcaaa atactgccgg tgtcgggatc
     1021 ggtaaaccag atgcgtaaac cataatgctc aatatcctgc cagcaacgca tacctaaaga
     1081 caccagccgc agatgatcaa gctttgcttc tccggcaatg ccagagccaa cgaccgtgcg
     1141 ccacggcaca ggaggaactt caccaacact gtcgctccgg gccatctctt gtgcgcaatt
     1201 taatcgactg tttaatgccg caagctgacg taagcattct ccggcatgat agtggctggc
     1261 gcgggcgtgg aaggcatcaa cgctggcgcg cagttgccgt agcgattcac tcacccatcg
     1321 ccagctgcag cgttccgctg cttgctgcgc gcggctgaaa gcggcctcgt agtgaataag
     1381 cggctggctg atgccgctca gccataatgc ctggcttagt tgttgaacat attgacgaca
     1441 cgttttgcct tcttcgctgg caaacggatc gtcagatgat gtgacgtgtt cgctgcgcat
     1501 ctgccagatt aaatgagtca attccgcttg ctgcgctttt gcctggacaa aggcctgtac
     1561 tgccagtacg acatgttcgc aaagtgtgcc ttcaatacaa tcacaacggg cgaaacgaat
     1621 actgctgcgg gaataaaaac gcacatcgct catcggtaag cgggcagagg gaatttcacc
     1681 cggcgcacag aacaactcaa tggtgatgct tttagcgacc agcgcctgtg cgcgtttgcg
     1741 ggtggcatcg ggaagggtag ccagttcttc cagccagatt gccggatccc attcctcttc
     1801 ttttcccgta ggctgggcgg tagcacaaag tcgttgataa cttaacacca gcatcacgcg
     1861 atgacggcac atgccgctgg ccccataggt gcactgagcc tctttcagtg cctggccgtt
     1921 cgccagctgg gtacggacac cgtcactgaa ggtggcgatt aaagcgctgt tctcatggct
     1981 gatttccggg acgttgccat tttccagttc cttaaggctg cgcttaacaa aaccggcatt
     2041 gcttaacgcc gtcagtgcct gcggtgtcag ttctaataat tccggacgta gtgaattcat
     2101 gactgaagat tctccgcaag ccatgatgcc agctcgcccg gtgtcatggc ggctatttgt
     2161 gcgccgacat taatcagcgc ctgggccgta tcgcggtcat agcaaggtgt tgctgtgcta
     2221 tcgagcgctg ccagtcccag cactttgatg ccgctctgga cacacttttt cacttggtgc
     2281 gtcagcaatg atgatgaacc cccttcataa aaatcgctca cgaggataat gacgcttttt
     2341 gcaggttgtt caataagttg ccgaccatac tccatggcac tggcgatatt ggttccgccg
     2401 cctaattgca ctttcattaa taactctacc ggatcggcaa cgtctgccgt gagatcaacg
     2461 acgcttgtgt caaacgccac cagatgggta cgaatgccgg gtaactgcca taaacaggcc
     2521 gccatcaccg cagagtggat caccgaatcg accatcgatc cgctttgatc aaccagtaag
     2581 accagttgcc attgctcgct ttggcgttta atgcggctgt taaagcgggg gaattcgata
     2641 tacaacttgc cgtgttgcgg gtgccagtgt tgcaggttgg cgcgcagagt acttttgaaa
     2701 tcaaagtttc gcgccagtgg aataaatgag cggcgacgac gatcgcggac accagaaaaa
     2761 gcctgacgaa cttcctttgc cagtcgcgcc ataatttctt ccacaacctg gcgcacaatc
     2821 ctgcgggcgg cagccagtac ttcggggttc atcagatgtt tggtgtgcaa aacggcgcgt
     2881 agcaggcttt cggaaggctg catacgttcc agcacgtcga gatttgtcac cacatcttca
     2941 atgccgtagc gcagtacggc atcgctttcc agccgctcaa tcacctgttg cggaaacagc
     3001 gtgtgaatac tgttgatcca ctcaggagtg gtgagatttg agccacctaa tccactagag
     3061 cgttcaccac gctggagccg ttcaggatcg cgcccataca gccactccag cgcgtgatct
     3121 atctgccggg cgttgtcatc cagcccacaa agcgtcgttt ctgccgcttc gccaagaatt
     3181 aatcgccagc gttgtagctc acgggtggtc agaagatcgt tcagttcaga catggaaaac
     3241 tccccagtgt tgcagcgatg ccagtgcctg ctgttcgagt tgttgatgat gtgcaatggc
     3301 ttgcggtgga caatgcaacg gcatttgcag tgccgaaacg ggaagttgcg ccagttgata
     3361 atgctcaagc acctgatgcg ccagcgtccc gcgttctcgt ggcggtagcc aggccattgc
     3421 cgcgcgtaaa tcgggcaggg cattggtaaa atcggcatca cttagttgat ttaaatgact
     3481 gctgaaaccg gcgataaatg ccggctgaca ggccagttga tgacgggcta atgccagcaa
     3541 accgtgcagc gcctcgccgg attgtgccgg ggataactgc gccagcatcg tcagcgccgc
     3601 ttcggcacta gcgttgggat gttccagacg catcagcgca ccaagagtcg cgccgcgatc
     3661 cagtgccagg acatgaatcg cctgactgtg tcgctccagc aaggctaccg ccgcagaaag
     3721 cgaaacgccg gggagttgca caccgctatg tagatcgcgc aggatatgac acaacgcttg
     3781 ccagctatgc aagtgagcgt gaaactcctt ttcatccggt ctgccgttgg attcacacag
     3841 ccacagcgtg cgatcgatag ccgcgcataa cgtcgtttgt aatatctgcg cgccttgcat
     3901 accgctaatt tcatccagcc gccataaggc atatagcact tccagcgcct ggcccatttc
     3961 ggcaaattga ttttcctggg cgattaataa tgtgagttgc tccagtaatt gttgactgaa
     4021 ggacgccaga cccgctgacg ccgcttggct aagacatgtg gtaatgctgc cgattccacc
     4081 cgcgtccagc atatcggctt ctaatttatg gcgtgcggct tcctggagtg tggcaccaaa
     4141 gcaggcggcc tcaatcaatg ccgcatgctg gctaagcggg cgggttaatt tccagcgttc
     4201 ttcaccgttg cctgcaagtg tcagtgtgct tccctgctgg cgtacaatcc ctgggatctc
     4261 cagtattgcc agccgatgta acacctgact ttgcgctagt ccattggggt taaagcgatt
     4321 taaggtaagc tccgccggaa gagagattgc ggtacgttcc agttcgcaag tgacatcttt
     4381 tggcagaggc ggttgtggcg tagaaggggc aagtttaccg aatccgtcac ccgctaatgt
     4441 gtctatcaac gttagcagaa tcggatcggt atcgggatga ataacgccgc gatagctcca
     4501 cggcaacggc gcattcaggg cttctttaat cagcgatcct gctatggcat ccagccagtc
     4561 agtgcgtaac ggtagtgtat gaccgcgcaa ctgtgccagt gccatcgcat gcagatgagc
     4621 ggcagccata tccgcggtcg aagcaggcaa cttgtgctgg cgcaaacggg tgagaaccgt
     4681 ttttagcagt tgttctcccg cctgctgtaa gccccactgc cagcaccagt tttgccagac
     4741 cggggcaggc attcctgaaa ggtatcctgc cagcacatca aggcgctttt cactgtaggg
     4801 ggtgagataa caacctgtaa cggcatctgc cagcgacggc aattctggcg tgttaattac
     4861 ctgcggacat tcgcgccaca tttttgccag tgccggagcg tgccagccac cgcagacaac
     4921 taacacgtcg ccattatttt gctgcatcgc ccatccaatc cagcgggcca taaaggcttc
     4981 gcgctgacga tttagagcat cgccggggga gtcgccccgc agttgggcaa aatagtgcgc
     5041 cagtgcagag ggtaatgcag tttgctggct ttcatcttcg aacaagtgat cccacagggt
     5101 gtcgctgtta tccatgcggg tggcacgcag cagcaaggtc tggctttcct cttgcgtatc
     5161 aggcgaatcg tcctcttctt cactctgcgc ccagcaaggc aaatcgatga agtaagtttg
     5221 tgcctgaata cgacgatcgg cttgtagcgc ctgccactcc ggcgaaaatt cagcaaatgg
     5281 cgtccaggca ccacgcccgg gggctgcacc gtcctgatac tggcaataac tgtaaatagc
     5341 taccggaagc tggtgggcta aaaacagttc gtctacccgg tcattaaaat cagcagggcc
     5401 ttcaatcaac acgtatcgtg gccgctggct ttcgattaac gatttcacca gccgggcgca
     5461 ggccggacta tgatgccgga tgccgacaat taacggctcg ctcatgcatc tttcctcacg
     5521 gcaggcggtg gcgggcttga taataagcct gccagtgagc ttctttatgt gtggcgacac
     5581 gctgttcgaa gtaacggcgc agacgagcgc gatcttcttc gttatctttg acgatggttc
     5641 cggcaatgca gtccaccagg tcggctggct cacctgcgcg attcgctaaa aaccaggcgc
     5701 ggacgcctac ggcgtgggcc acgttcacgg cttcagcggt ggacataatc gctgttagtg
     5761 tatccattga cgttttcttt tcgccgttgg cacgcagatc gcggaaggtg cggaccagta
     5821 actccagtac cgcgtcaggc actttatgcg gaataccgct atgggccagc aaacgcgccg
     5881 aagcactggc gacaagctcc agctcctggg cgaaatccat aatcggaaag acagtttcaa
     5941 aatcgaagcg acgctttagc gcagcactca tttcattgac gccgcgatcg cgggtattgg
     6001 cagtggcaat aatattaaag ccttctcgcg catagagctg actggcttca ccagtgagtt
     6061 ccggcaccgt catcaccctg tcagaaagca tacctaacag acagtcttgc acttccagcg
     6121 gtgtacgcgt tatctcttca aaacggacga ttttgccgtc gcgcattccc tggtaaagcg
     6181 gggcagggac cagcgcttcc gttgatggac catgattgat aagcagggcg tagttccagc
     6241 cgtacttgat ttgatcttcg gtagtagatg cgccgccctg gatggttaac ccggcatcgc
     6301 cgctaataga ggttgccagt aattcagaca ggagagattt tgcggtgcca ggttccccca
     6361 ccaacatcaa accgcgtccg gtggcaagag tgactaacat acgttcgaca gcggagggac
     6421 taatcaccac tttacggcta atattttgcg cgctatcgcc aaggataaaa gcacgggccg
     6481 caggcaaact taactgccaa ccgggcgggc aaggtgcgtt atcattttgt tttaattttg
     6541 ccagttcatc ggcgtataac acagcagcag gcggacgctg c
//
LOCUS       DAJTQY010000154         6408 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.154, whole genome shotgun sequence.
ACCESSION   DAJTQY010000154 DAJTQY010000000
VERSION     DAJTQY010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6408
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.154"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            59..1385
                     /gene="pgaC"
                     /locus_tag="OO349_003695"
                     /pseudo
     CDS             59..1385
                     /gene="pgaC"
                     /locus_tag="OO349_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415541.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine synthase"
     gene            1387..1800
                     /gene="pgaD"
                     /locus_tag="OO349_003696"
                     /pseudo
     CDS             1387..1800
                     /gene="pgaD"
                     /locus_tag="OO349_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309294.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     biosynthesis protein PgaD"
     gene            complement(1850..2638)
                     /gene="phoH"
                     /locus_tag="OO349_003697"
     CDS             complement(1850..2638)
                     /gene="phoH"
                     /locus_tag="OO349_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HCR5868616.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMSGVEA
                     IGMARDSRDTSPILARNEAQLHYLQAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDLLVRRLGASFMQYCLRPEIG
                     KVETAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(3246..4511)
                     /gene="efeB"
                     /locus_tag="OO349_003698"
     CDS             complement(3246..4511)
                     /gene="efeB"
                     /locus_tag="OO349_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HCR5868617.1"
                     /translation="MQYKDENGVNEPSRRRLLKGIGALAGSCPVAHAQKTQSAPGTLS
                     PDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLSQG
                     GAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKMTR
                     FPNDSLDAALCHGDVLLQICANTQDTVNHALRDIIKHTPDLLSVRWKREGFISDHAAR
                     SKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQFR
                     VEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLANPR
                     TAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALEEY
                     VKPIGGGYFFSLPGVKDANDYLGRALLQV"
     gene            complement(4517..5644)
                     /gene="efeO"
                     /locus_tag="OO349_003699"
     CDS             complement(4517..5644)
                     /gene="efeO"
                     /locus_tag="OO349_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HCR5868618.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(5702..>6408)
                     /locus_tag="OO349_003700"
     CDS             complement(5702..>6408)
                     /locus_tag="OO349_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HCR5868619.1"
                     /translation="TSRSRQAAYSGIFINETTGEFPQKEQELFEGIVAVIAVVILTWM
                     VFWMRKVSRNVKVQLEQAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQ
                     DVGIWPPLGAMLGLATAVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRA
                     FHEAGLWNHFQEIAFDMSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALV
                     AFALPPRAGATASRSA"
BASE COUNT         1540 a         1690 c         1545 g         1633 t
ORIGIN      
        1 aaccaacctg aaatagacct tattcgtcct gagttttcaa cagcctggta tccgaaaaat
       61 gattaatcgc atcgtatcgt tttttatatt atgtctggtg ttatgcatac ccctatgcgt
      121 agcgtacttt cactctggtg aactgatgat gaggttcgtt ttcttctggc cgttttttat
      181 gtccattatg tggattgttg gcggcgtcta tttctgggtc tatcgtgaac gccactggcc
      241 gtggggagaa aacgcaccag ctccccagtt gaaagataat ccgtctatct ccattatcat
      301 tccctgtttt aatgaggaga aaaacgttga ggaaaccata cacgccgctt tagcacagcg
      361 ttatgagaac attgaagtta ttgccgtaaa tgacggttca acagataaaa cccgtgccat
      421 cctggatcgc atggctgcac aaattcccca tttgcgggtc attcatctgg cgcaaaaaca
      481 ggggaaagcc attgcgctta aaaccggagc tgccgcggcg aaaagtgaat atctggtgtg
      541 cattgatggc gatgcgttat tagaccgcga tgcggcggca tatattgtgg aaccgatgtt
      601 gtacaacccg cgtgtgggtg ccgtaaccgg taatcctcgt attcgaacac gttctaccct
      661 ggtgggtaaa attcaggttg gcgagtattc ctcaattatt ggtttgatca agcgaaccca
      721 gcgtatctat ggaaacgtat ttaccgtttc cggtgttatt gccgcatttc gtcgcagcgc
      781 cctggcagaa gtgggttact ggaatgacga tatgatcacc gaagatattg atattagctg
      841 gaagctgcag ttgaatcagt ggacgatttt tttacgagcc acgggcgctg tgctggatat
      901 taatgcctga aacgttaaaa gggctgtgga aacagcgcct gcgctgggct cagggcggtg
      961 cagaagtatt cctcaaaaat atgacaaggt tgtggcgcaa agaaaacttt cgaatgtggc
     1021 cgctgttttt tgaatactgc ctgacgacaa tatgggcctt cacctgcctg gtcggtttca
     1081 ttatttacgc agtccaactt gccggtgtac cgttaaatat tgaattgaca catatcgctg
     1141 cgacacatac tgccggaata ttattgtgta cgttatgttt actgcaattt attgtcagcc
     1201 tgatgatcga gaatcgctat gagcataatc tgacttcatc gcttttctgg attatttggt
     1261 tcccggttat tttctggatg ctgagcctgg caacgacatt ggtatcattt acacgagtca
     1321 tgttgatgcc taaaaagcaa cgcgcccgtt gggtaagtcc tgatcgcggg attctgagag
     1381 gttaatatga acaatttaat tattacgacc cgacaatcac cagtacgttt actggttgat
     1441 tatgttgcca caaccatctt gtagacatta tttgctttgt tcatattctt attcgccatg
     1501 gatctgctga cgggttatta ctggcaaagc gaggccagaa gccgacttca gttctatttt
     1561 ttgctggcag tggcgaatgc cgtcgtgtta attgtctggg cgctgtacaa taagctgcgt
     1621 tttcaaaaac agcagcatca tgcagcctac caatatacgc cgcaagaata tgcagagagc
     1681 ttagcaatac ctgatgagct ctatcagcaa ctacaaaaaa gccacaggat gagcgtacac
     1741 ttcaccagcc aggggcaaat aaaaatggtt gtttcagaaa aagcgctagt ccgggcataa
     1801 acacccaaaa caaagcccgg ttcgcccggg ctctgcaccg ataatacact taactgtatg
     1861 catgcagcgt acgttggcaa agtgccgaac gtacgcaatc ctcttttccg aaccggacga
     1921 tcccaaccat ttcatcttct tcgaaacgtt ccagcgcgtc acttaatccg gagcacacgc
     1981 cgcgaggcaa atcgcattgc gtgatatcac cgttgacgat aaccgtcacg ttctccccga
     2041 ggcgggttaa aaacattttc atttgcgcgg cagtcacatt ctgcgcctcg tcaagaatga
     2101 caactgcatt ttcaaaggta cgtccacgca tataggcgaa cggcgcggtt tccaccttcc
     2161 ctatttccgg tcgcaggcag tactgcataa aggaagcccc taagcgccgg accagcaagt
     2221 cgtagaccgg gcgaaaatag ggagcaaact tttctgcgat atcaccaggt aagaagccaa
     2281 gatcttcatc ggcttgcaga actggacggg tgacgataat cctgtcgaca tccttatgta
     2341 tcagggcctc tgccgctttt gctgcgctga tccaggtttt tccgcacccg gcttcgcccg
     2401 tggcgaatat cagctgctta ctctcaatag cctgcagata gtgcaattgc gcttcatttc
     2461 gcgcaaggat gggcgaggta tcgcgactgt cgcgggccat accaatggct tctacgccgc
     2521 tcatctgcac aagcgaggtg accgattctt cttcacgctg cttatggctg cgcgaatccc
     2581 gtctcagcac acgttttgcc tcgcgacgag ctttgatcac tgctttttgt cttcccatgg
     2641 agagcacctt gagttgtttg tattcatcac acgcgccgtt ggcagcgcga ttatgcgcac
     2701 gaacatcaga gggttggctt ccttgtaagc catagtttgc ttttggataa aatgccgaaa
     2761 aacggctacg cacaccgttt gcggcgtcgg taacacgagt aaagaaagga agtgagaaaa
     2821 atacagagtg acgaagttgc taccggagga gaaatctccg cgagtggtgg agcgttgctt
     2881 aacaaaacat tgtccagaac aggacgttac catccgcgat ctccatagtg actgactatc
     2941 actgccggga acttccgctg ctacttaata agtacaacag aactcgcatt cattgcaaca
     3001 atatattcac ttatatttaa cgataaacac ccatttcggt gacattaatt tctactggaa
     3061 agatgacaga gtgatgacag tgatggaaaa agctgtgtgc tttccgcagg attcgcaggg
     3121 ctggtaaaat gtgcctcgtg tttgccggat tcggcgcgaa cgccttaccc gacctacaag
     3181 tgattaataa tttgtgcaca aaactcatgc ccgataaacc tgcgcatcgg gcatatttgc
     3241 gtcgttcaaa cctgcaatag cgcccttccc agataatcgt tcgcgtcctt cactcccggc
     3301 agcgaaaaaa aataaccgcc gccgataggt ttaacgtatt cctccagcgc ttcgccattg
     3361 agcctttttt gtactgtcag gaagcctttt tccagatcgt gttggtagca gacaaacagc
     3421 aaccccatat ccagttgtcc ggagttggtg acccccaacg aataactgta gccgcgacgc
     3481 agcatcaggc tggactcact ctccgccgtg cggggattcg ccagccggat atggctgtcc
     3541 agcgcgatca ccttcccttc cgggtcgctg gcgtaatcag gcacatcatg ctcatgctgc
     3601 attcccagcg gcgcaccggt ttgcttatca cggccaaaaa tcgtctgctg ttctttcagc
     3661 ggcgttctgt cccaaaattc cactcgaaac tgaatcaagc gtactgcctg atagctgcca
     3721 ccgattgtcc acgcaggctc ctgctgatct gccgttaccc acaccacttt ttgcatcaac
     3781 ttatcattct ggctatcggg attggcagtg ccgtctttga aacccagcaa attaatcggc
     3841 gtctctttgc ctttactacg cgccgcgtga tcggaaataa acccttcccg cttccagcgc
     3901 acactgagca aatccggcgt gtgtttgatg atatcgcgca gcgcatggtt caccgtgtcc
     3961 tgggtgttgg cgcaaatctg tagcaacaca tcaccatgac ataacgccgc atccagcgag
     4021 tcgttgggga aacgcgtcat cttctgcagc ttttttggca tctgtggcgc aaggccaaag
     4081 cgctcatcaa acaatgagtg acccaccgat aacgtgatgg tgagattatc gggcgcaatg
     4141 tagccgccaa gaatgccgga atcgagtggt ggcaggcgcg gatttggcgt ttctggtgct
     4201 gctccgccct gactcagaaa agcaaaacgc tgagtcaaca aacgaaacaa ccgctcaaga
     4261 tcggctttat cgctggcaag cacatcaaac gccaccagca tcattgcggc ctgttgtggc
     4321 gtcaggatcc ctgcctgatg ctcaccataa aacggctgtt tctcattgcg tgcatccggt
     4381 gaaagcgtgc ccggtgcact ttgcgttttt tgtgcatgag cgaccggaca acttcccgcc
     4441 agcgcaccaa tccctttcag taaacgtcgg cgtgacggtt cattcacgcc gttttcatct
     4501 ttatactgca taacgcttaa tccagtccca gcacaccgcg aagttgcgcc agatcttccg
     4561 ccagcgcagt aatcggccct ttcagcgcat tccggtcggc atcggtcaat ttgtcgtagg
     4621 tttcaaaacc gtctttagta cggtatttcg ccagaatggt atcgaccttt ttaaagttgg
     4681 catcgacttt tgccaacagt tccgggttgg ctttttgcag ttgtggacgc agcagatcga
     4741 caattttctg cgagccttca acgttagcct ggaaatccca cagatcggtg tggctgtagc
     4801 gatcttcttc accgctgatt ttgctggctg ccacttcctc aatcagtccg gctgcgccgc
     4861 cgaccacttt tgaaggtggg aaagccagtt cactgatgcg tttttgcaaa tcgaccacat
     4921 cggtataaag ctgctcagcg tactgatcca tccctttggt ggtgttgtcg ccaaacaatg
     4981 ctttttccag acggtggaaa ccagtgaatt ttgggtcggc ggctttttgc tcgtaatcat
     5041 cttcacgggc gtcaatgctg ccatccagat cggagaacag ttcagcaatc ggttcaatac
     5101 gctcatagtg ctggcgcgtc ggcgcataca gtgctttcgc tttttcgata tcgcctgctt
     5161 taatcgcgtc ggtaaaggct ttggtgtcgg tcaccagctg cgtggtttcc gccatgacat
     5221 acgctttata tgcagtaatt gcaccaccaa gacttaacag cgcatcactt tgcgccgcat
     5281 ccgccgttgc ctcacctttg acgatcaact tccctttcgg gttagtcagc agaccgcagg
     5341 tcatatcgta ttcgccaggc tgtaaattcg ccgtcatttt ctggctaaaa ccaggggcga
     5401 tattttcccg ctcttccacc accatcacgc ctttgaggat ctcccactcc agcgccttct
     5461 ggctatggtt ctgaataatg aactgtgttt tcccggcgtt aaccgtaatg gtcatcggtt
     5521 cgcactgctt atccgtcacg gtcactttga cctgcggcac atcagcggcg ttagccataa
     5581 aagcagaaga aaacagcgca gccacgctca actgcaatgc gttacggcgg aagttaatgg
     5641 tcatgacgag actatccctt taaagtatgt tgtaactaag caagagtttg cgtcgtattt
     5701 gttacgcgga gcgagacgct gtcgcccctg cgcgtggtgg cagagcaaat gccaccagcg
     5761 ccgggatgag ataaataaac cagacggcga cttcgctgac gctcggtgct tcctgatagc
     5821 caaaaatccc ttccatcagc gtgccaaaca gcgagtgagt tgagaggacc gcgctcatat
     5881 cgaaggcgat ttcctgaaag tggttccaca atccggcttc atgaaatgcg cgaatggcac
     5941 cagccgccag ccctgcggcg acgaagagaa taaacaggct ggtccattta aaaaatgcac
     6001 caagattgag gcgaataccg ccccagtaga gcaggaagcc gagcaccacg gcagtagcaa
     6061 gaccgagcat tgcacccagc ggcggccaga tcccgacatc ttgttgaaat gccgccagca
     6121 ggaaaaagac cgactccagc ccttcccttg caacggcaaa aaagaccatc atcaccagcg
     6181 cccagccatg atgatttcca cgctgcaatg cgctatcgac tgcctgttcc agttgcactt
     6241 tgacgttgcg cgacactttg cgcatccaga aaaccatcca ggtaaggatc accacggcga
     6301 tcaccgccac gataccttca aacagttcct gctctttttg cggaaattcg ccggtggttt
     6361 cgttaatgaa gatccccgag taggcagcct gacggctgcg gcttgtca
//
LOCUS       DAJTQY010000155         6403 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.155, whole genome shotgun sequence.
ACCESSION   DAJTQY010000155 DAJTQY010000000
VERSION     DAJTQY010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6403
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.155"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(13..1227)
                     /gene="rspA"
                     /locus_tag="OO349_003701"
     CDS             complement(13..1227)
                     /gene="rspA"
                     /locus_tag="OO349_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HCR5868620.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPRLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNR"
     gene            complement(1433..1759)
                     /locus_tag="OO349_003702"
     CDS             complement(1433..1759)
                     /locus_tag="OO349_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HCR5868621.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLSLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            1894..2235
                     /locus_tag="OO349_003703"
     CDS             1894..2235
                     /locus_tag="OO349_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HCR5868622.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            2301..2830
                     /gene="speG"
                     /locus_tag="OO349_003704"
                     /pseudo
     CDS             2301..2830
                     /gene="speG"
                     /locus_tag="OO349_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226795.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
     gene            complement(2833..3543)
                     /locus_tag="OO349_003705"
     CDS             complement(2833..3543)
                     /locus_tag="OO349_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HCR5868623.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            3651..3956
                     /locus_tag="OO349_003706"
     CDS             3651..3956
                     /locus_tag="OO349_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HCR5868624.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYNRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            4155..4478
                     /locus_tag="OO349_003707"
                     /pseudo
     CDS             4155..4478
                     /locus_tag="OO349_003707"
                     /inference="COORDINATES: protein motif:HMM:NF016750.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5034..5612
                     /locus_tag="OO349_003708"
                     /pseudo
     CDS             5034..5612
                     /locus_tag="OO349_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606644.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
BASE COUNT         1706 a         1628 c         1501 g         1568 t
ORIGIN      
        1 ttatcttact ccttacctgt tccacagcgt gccatcttcc agacgtgcga ctggtagata
       61 agcaggttca tagggatatt tcgccgccag cttttcatcg aattcgatac caagacccgg
      121 tttgtctccc ggatgcatat agccgttatc gaaagtccag ttgtgcggga agacttcgag
      181 catttgttcg gaataaccca tgtattcctg gacaccgaaa ttggggaccc acagatcaaa
      241 gtgcagcgcc gcagccatgc agactggtga caaatcggaa ggaccgtgtg agccagtacg
      301 tacctgatac agcgaagcaa aatcggcaat ccggcgcata ccggtaattc cgcctgcatg
      361 ggtcagcgtg gtgcggatat aatcgatgag ttgctcttca atcagttgtt tgcagtccca
      421 gatgctgttg aagacttcac ccactgcgat gggtgtgacg gtatgttggc gaatgagacg
      481 gaagcattct tggttttccg ctggcgtcgg gtcttccatc cagaacatgc gataatcttc
      541 aatgctttta ccaaagcgcg ccgcttcaat aggcgttaag cgatggtgca tgtcatgcag
      601 caaatgttca ttaaaaccaa acttgttacg taccgcgtca aacaatttcg gcatgaaatc
      661 gaggtatttc tccgtcgacc acagctgctc ttccggccac tgtcctttgg ttgcgggttc
      721 ataagccaga cctttacctt tcgacatgcc gtaggtggtt ttcataccag ggattccgca
      781 ctgcacgcgg atggctttga atcccagctc ctgatgacgg gcataatcat ccagagcttc
      841 atcaatactg tgaccggtgg tatggcaata aaccatcacc ccttcacgag acgcgccgcc
      901 gagtaactgg taaagcggca tgttggcagc tttggcttta atatcccaca gcgccatatc
      961 aaccgctgaa atggccgaca tcgtaaccgg accgcgacgc cagtaagcac ctttatagaa
     1021 aaactgccag atatcttcga tacggtgcgc atcgcgacca ataagccgcg gacaaagatg
     1081 atcctgcaaa taagaggcca cggaaagctc acgtccattg agggtggcat ccccaaggcc
     1141 cgtaataccg tcctcagtgg tgatttttaa tgtgacgaaa ttacgccccg gacaggtaac
     1201 aaaaacttca gcctttacga tcttcatgtt cgattccttg catcgcttgt cgtgatgcat
     1261 gaaatctacg caactgagct actgccatac aagtatgaag atcgaaaaaa gccggagtga
     1321 tcacaaaaaa aggcgtatat ttgcgctgtg aatggttgac aaaagatgaa atagaatacc
     1381 ttttgtcagc tgacacttcc tcgtatctta ttgataaaat ggatttatgt tcctacgtgc
     1441 gcccccagcc cgcaacaatg atcaacattc cgcaaagcgc aatcaacgca cccgtccagt
     1501 cataaagact tagtttcacg ccatccacaa cgcgcagcca catcaatgcc gtgcagacat
     1561 aaacgccacc ataagccgcg taaacacgcc cactcgccgc aggatgcaac gttaacaacc
     1621 agacaaacag cgccagtgaa atccccgccg gaagcaacag ccagatactg gcgtttcgtt
     1681 ttaaccacaa ccagggcaga aagcatccaa taatttcaca cagcgcagta gcaaaaaata
     1741 gtaacgttgt tttaatcatc tttgtcactt attgacatca tgtatagtta tagggcgaca
     1801 taatatcatc aatataaaca tcctcctggt acgttttgcg ttcgcagtgg atggtgtaga
     1861 atcaccttta atcattcata cagggaatga attatgaaaa tcactctcag caaacgaatc
     1921 ggcctgctcg cttttctgct gccttgcgca ctggcattga gcacaactgt tcatgccgaa
     1981 actaacaaac tggtgattga gtctggcgac agtgcacaaa gccgccagca cgccgctatg
     2041 gaaaaagagc aatggaatga cacgcgcaat ctgcgccaga aagtgaataa acgcactgaa
     2101 aaagagtggg ataaagccga cgccgctttt gataaccgcg ataaatgtga gcaaagcgcc
     2161 aacatcaatg cctactggga gcccaatact ttgcgctgcc tggaccgtcg aactggccgc
     2221 gttattaccc cctaacctgt tattgattta aggaatgtaa ggacacgtta tgccaagcgc
     2281 ccacagttaa gctacgcccg ctggagcgtg aagatttacg ctatgtacat caactcgaca
     2341 ataacgccag tgtgatgcgt tactggtttg aggaacccta cgaagccttt gttgaactct
     2401 ctgatctgta tgataagcgc atattcacga tcagagcgaa cggcgctttg tggtggaatg
     2461 tgacggcgaa aaagccggtc tggtggagct ggtggaaatt aaccatgttc atcgccgggc
     2521 agaatttcag ataattatct ccccggagta tcaggggaaa ggtctggcaa cccgtgccgc
     2581 caaattagca atggactatg gcttcaccgt tctcaatctc tataagctgt atctgatcgt
     2641 cgataaagag aatgaaaaag cgattcacat ttaccgcaag cttggcttta cggttgaagg
     2701 tgaattgatg catgagtttt ttattaatgg tcaatatcgt aatgccattc gcatgtgtat
     2761 attccagcat cagtatctgg cagagcacaa aacaccgggt cagactctcc tgaagccgac
     2821 cgcacaatag cattaataat aatcgatcgt atttttgatg gtgtaaaccc gttcgacggc
     2881 gggttttact ccttcatcaa caatgatcag ctgacagtcc actgggttgg cgtgactgtc
     2941 atattcacag ctctgtttta cattaccaac tcgttgatta tttagtaaag taaccgctgt
     3001 gtaatctaat tttttgatcg gatccgttga tggcgtggcg ctgacagata atgttttgtc
     3061 gttacttttc gtggttttac ccagcggata cccctgatca tcatagcgat attccatctg
     3121 catttgtttg ctgctggctt taatcacgaa gccattatca tcggtttccc aactcacccc
     3181 ggcagaaggt aattctgcta gctggcattt tccctgtaaa cgtactctct tctccagcgt
     3241 ctcggcatca cggtaataat tggcgtccag taccagagcg accacggtat tattttccag
     3301 atccagtaat tcgagtgaat caaaacagcc ttcttccgac aaagtcccag aaacacgttt
     3361 cgtcacttca ccttgctcat ccattaatgt ctgagtgaaa tcttttaccg gaccacgcag
     3421 cggatcaaaa tcgaattcat tagagaaact ggccatctca ggggtaaatg aaagtgttac
     3481 ctctgtgcgg tcacatcctg tgaggaatat cgcgagtaag catggtagta atttgtattt
     3541 cacaacagtc accagagagt agagatgatt ctcaatcata gtagcaaata cagtacttta
     3601 cacgttaaat gctatgctta aagaagttat cttcgcgtaa ggagcttatg atgaaactct
     3661 caacctgctg tgccgcgctt ctgctcgccc tcgcctcacc cgcggtactc gccgcgccgg
     3721 gatcttgtga gcgcatacag agcgatatat cacagcgcat tatcaataat ggcgtacccg
     3781 aaagcagctt cacgttaagt attgtaccca atgaccaggt tgatcagcct gattcccagg
     3841 tcgtcgggca ttgcgctaat gatacgcata aaattctcta caaccgcacc accagcggta
     3901 acgtctctgc tcccgcgcag tctacccagg atggcgcgcc tgccgaaccg cagtaacaca
     3961 ttatcgactg aacgccggat atgacacatc cggcgatttg aacatacaac ataatctcac
     4021 cttattactc atacccttct attgatgtgg attaataatt cttaacccaa aatgggtaga
     4081 ctccctctat tgttagcgcg ctaaatattc aatatataaa catttatata acaataaaga
     4141 acagggagtg agttatgtcc aaaaatgacc gaatggtggg catcagccgc agaacactcg
     4201 ttaaatcaac cgcgataggt tctctggcgc tggctgcagg cggtttttct ttaccgttca
     4261 ccctgcgcag tgcagcggca gcggtacaac aggccagcga aaaagtgatc tggggttcct
     4321 gttccgtcaa ctgtggtagc cgctgtgcac ttcgtctaca tgttaaagat aatgaagtga
     4381 cctgggtgga aactgacaat accggcagcg atgagtacgg caaccatcag gtacgcgcct
     4441 gtttgcgcgg tcgctccatc cgccggcgta ttaatcatcc cgatcgcttg aatgacccaa
     4501 tgaaacgcgt gggtaaacgc ggtgaaggca aattcgaacg gattagctgg gatgaagccc
     4561 tggatactat cgccagtagc ctgaaaaaaa ccgtcgaaca atatggcaat gaagctgtat
     4621 atattcagta ctcttcgggg attgttggcg gcaatatgac ccgctcttcg ccatcagcct
     4681 cggcggtcaa acgcctgatg aactgctacg gcggttcact caatcagtat ggctcctaca
     4741 gcaccgcgca aatttcctgt gccatgccct acacctacgg cagtaatgat ggcaacagca
     4801 ccacggatat tgaaaacagc aagctggtcg tgatgtttgg taacaacccg gcagaaaccc
     4861 gcatgagcgg tggtggcatc acttatcttc ttgaaaaagc gcgcgagaaa tcgaacgcca
     4921 aaatgattgt tatcgatccg cgatataccg atacggctgc cggtcgtgaa gaggaatggc
     4981 tccctattcg cccgggcacc gatgccgcgc tggttgcggg tattgcctgg gtattgatta
     5041 acgaaaatct cgttgatcaa ccttttctcg ataaatactg cgtcggctat gacgaaaaaa
     5101 ccttacccgc agatgcaccc aaaaatggtc actataaagc ctatattctt ggtgaaggtg
     5161 acgataaaac agcaaaaacg ccgcagtggg cttcgcaaat taccggtatc ccggtagacc
     5221 gtatcatcaa actggcgcgt gaaattggca caacaaaacc cgcttatatc tgctagggct
     5281 gggggccgca acgccaggca aacggcgagc tgactgcacg cgctattgct atgttaccta
     5341 ttttgacggg caatgtcggc atcagcggcg gaaatagtgg cgcgcgtgaa tcgacctata
     5401 ccattacgat agaacgccta ccggtgctgg ataatccggt caaaacgtca atctcctgct
     5461 tcagctggac ggatgctatc gatcatggcc cgcaaatgac ggcactccgc gacggtgtcc
     5521 ggggcaaaga taaactggat gtgcccatta agttcatctg gaacttcaag gacttgaagc
     5581 ggacccttag taggcctgag gtcctgcatt gagtcaagac cttgtgtcat tctttgtgcc
     5641 agacttgtgt cattagacgg gatgtctaga cggctggtca gcccccccgg tggcctcggt
     5701 ctggacttga ggtgggccta tggggggaag tctgctggct taatgtatta gatggtcatt
     5761 cacatttttc tgtcagaaac catcggtccc actgtgagtc caaacctaag gacctagaag
     5821 tccacccaca gccttagaca gagtgtcgaa cacatccaca ccgagtcgac cagagaggac
     5881 catgtagaga cccacagaga ggaacacgag tttatagacc agagtcagga gggacttcag
     5941 gtctgcggaa gtcatagaca taaagcccct atctgttatg ttatattatt atattaacaa
     6001 caggtccaga ccattggtcc agaccatagt tagttaatca cagtgatagc ccaccacctg
     6061 aagtcctgct gatattcagt ccagacttac ctctgctcat ggtcttacct ccctcggtct
     6121 cctgaccttg ggtatcgact acagcttgct gagcgtcatc tggaggagct acaggaggcg
     6181 ggaccgaagc cccaccttga gtcccaccac cacctgtcag gcccttggcg atggacctga
     6241 agcctttact aatgctctta cccattggtc ttacgcttcg gtcttggtga acttcaggcg
     6301 tttgactaca ggcaggacgg cagtagctga gccagtgccc atcttgacgg tcgtagcagc
     6361 ttcagcgaca acgaacactt taccgaggat ttccaagtcg tgg
//
LOCUS       DAJTQY010000156         6356 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.156, whole genome shotgun sequence.
ACCESSION   DAJTQY010000156 DAJTQY010000000
VERSION     DAJTQY010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6356
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.156"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            <1..371
                     /locus_tag="OO349_003709"
     CDS             <1..371
                     /locus_tag="OO349_003709"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5868625.1"
                     /translation="TTLMSWDAVLYDLRSEKIISKDNYLTDLNRRHLELVLHTNPNPS
                     GSVTKILRTIFTKQVKSLGPELCHFLHHELTKKRYEVWHHYDVLHYQSSSFSRSQFNA
                     LINLLAQQSRFADLKLSNYK"
     gene            complement(459..1103)
                     /gene="cheZ"
                     /locus_tag="OO349_003710"
     CDS             complement(459..1103)
                     /gene="cheZ"
                     /locus_tag="OO349_003710"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HCR5868626.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTHMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(1114..1503)
                     /gene="cheY"
                     /locus_tag="OO349_003711"
     CDS             complement(1114..1503)
                     /gene="cheY"
                     /locus_tag="OO349_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HCR5868627.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(1518..2567)
                     /gene="cheB"
                     /locus_tag="OO349_003712"
     CDS             complement(1518..2567)
                     /gene="cheB"
                     /locus_tag="OO349_003712"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HCR5868628.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLATISAGQAIRI"
     gene            complement(2570..3430)
                     /gene="cheR"
                     /locus_tag="OO349_003713"
     CDS             complement(2570..3430)
                     /gene="cheR"
                     /locus_tag="OO349_003713"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HCR5868629.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEDRVWSAAASTGEEPYSIAMTLADTLGTVPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQTTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(3449..5050)
                     /gene="tap"
                     /locus_tag="OO349_003714"
     CDS             complement(3449..5050)
                     /gene="tap"
                     /locus_tag="OO349_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HCR5868630.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNHGE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSHEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMTDIVSSVTRVND
                     IMGEIASASEEQRRGIEQVAQAVSQMDQVTQQNSSLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(5096..>6356)
                     /locus_tag="OO349_003715"
     CDS             complement(5096..>6356)
                     /locus_tag="OO349_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HCR5868631.1"
                     /translation="TELIDYLDYGNTGAYFAQPTQGMQNAMGEAFAQYALSSEKLYRD
                     IVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLTPLAKIIAHICEIAGGNLA
                     NTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIYAGTREIAAGNTDLSSRTE
                     QQASALEETAASMEQLTATVKQNADNARQASQLAQSASDTAQHGGKVVDGVVKTMHEI
                     ADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVAGEVRNLASRSAQ
                     AAKEIKALIEDSVSRIDTGSVLVESAGETMNNIVNAVTRVTDIMGEIASASDEQSRGI
                     DQVALAVSEMDRVTQQNASLVQESAAAAAALEEQVSRLTQAVSAFRLAASPLTNKPQT
                     PSRPASEQPPAQPRLRITEQDPNWETF"
BASE COUNT         1441 a         1837 c         1524 g         1554 t
ORIGIN      
        1 acactacgct aatgagttgg gatgccgtgc tctatgatct acgtagtgaa aaaattatca
       61 gtaaagataa ttacttgacc gacttaaacc gcagacatct tgaacttgtt cttcatacta
      121 accccaatcc aagcggttcg gtaacgaaga ttttacgcac tatttttact aagcaggtaa
      181 agagtttagg gccggaactc tgtcatttct tgcaccatga actgacgaaa aaacgttatg
      241 aagtatggca tcactacgat gtactgcact accagtcatc gtcattttct cgttcacaat
      301 ttaatgcatt gataaattta ctggcgcaac aatcacgctt cgctgatcta aaattatcaa
      361 actacaaata accatcctcc acgtactccc tgaacgatac ccgccaccct gctatcggct
      421 tggcatcatc gcgcaggccg aagttcaggc aatacaaatc aaaatccaag actatccaac
      481 aaatcgtcca cctgatcctg actggctacc acgccggctt tgctggtatc gacctgaggt
      541 ccattaagca aactctggtt ttcacgtttt ggacgcgact cctgttccgg gatgttttcc
      601 aacagcacca tcagcaactg gcgttcgatc tcctgaatga catccatcat ccgcttaata
      661 acctgcccgg tgagatcctg aaaatcctgc gccatcatga tttccagcag ttgcgcgtta
      721 gtaaagctgg tatgcgcggg tacatctgcc agaaattgtc gtgtatctgt taccagttca
      781 cgggcgtcgg caaggtcaat cggatcggca aaccagtcat cccaacgttg ggttaacgct
      841 tttgctgatt tctccatttg atcctgatgc ggttgtgacg cctcaacact gttcagcgcc
      901 cgctccgcag cctgggcggt catctgcaca acatagtaca aacgatcgcg cgcatcgggg
      961 atggcttccg ccgcttcggc aatggcctga tccagcccca gttcccgcaa actgtcgcgc
     1021 agcatatgcg tcaggctgcc gatgcgcgca atgatatcgc cagctgaatg ctcgtcagca
     1081 ggtttgattg atggttgcat catagtcgca tcctcacatg cccagtttct caaagatttt
     1141 gttgagtttt tcctccagcg tcgcggcggt aaatggcttc accacatagc cactggcccc
     1201 cgcttgcgcc gcagcaatga tgttctcttt cttcgcttct gcagtcacca ttaacactgg
     1261 caatgccgac atcgcgccat ccgcacgaat tgttttcagc aattccaggc catccatatt
     1321 gggcatgttc cagtcggaga taacaaatcc ataaccgcct gcctgcaact tattgagagc
     1381 gtcgacacca tcttccgctt cctcaacatt attgaatccc agctctttca gcaggttacg
     1441 cactatgcgt cgcatggtgg aaaagtcatc cacaaccaaa aatttaagtt ctttatccgc
     1501 catttcacac tcctgattta aatacgtatc gcctgtccgg cactaattgt tgccaacatt
     1561 tgctggctta cctggctaag atcgaccact tcgcagacac cacccatatt gatggcctcg
     1621 cgcggcatgc cgaacaccac gcaacttgct tcgttttgcg caagggtcca tgcccccgcc
     1681 tgacgcatcg ccaacattcc cgccgcgccg tcgttgccca taccggtcag gatcacccca
     1741 accgcattac gccccgcctg tttggcgaca gaatggaaca acacatctac cgaaggccga
     1801 tgacggttaa ccgccgggcc gtcgtgaatt ttgatttgat aatttgcgcc actacgcgcc
     1861 agttccatat gccgatcgcc cggcgcaata taggcatgcc ccggcaggac acgttctccg
     1921 tcttcggctt ctttaacccc gatctggcaa agcttattaa gtctgtcggc aaaagagcgg
     1981 gtgaaaccgg gcggcatatg ttgggtaatt aacaatgccg ggctggaaag cggcaacggt
     2041 tgcagtacgt gacgaattgc ctcagttcca cccgttgaag caccaatcgc aatcagtttt
     2101 tcagaactca acaacggccc tgccttcagc gttgtcggtg ctgacaatgg cttatgtgct
     2161 gcaaggctcg cctttgctgc cgtacgcacc ttttcagcaa tcatttcgct atacgccagc
     2221 attccttcgc gaatacccag ttgcggtttg gtgacaaaat ctattgcccc cagctccagc
     2281 gcgcgcagcg tgacttctga tcctttgccg gtcaaggaag aaaccatcac aacgggcatt
     2341 ggacgcaaac gcattaattt ttcgaggaaa tccagtccgt ccatccgcgg catttcaaca
     2401 tccagcgtca gcacatcggg attgaatttc ttaatcaagt cacgcgcaac cagcggatca
     2461 ggcgcggtcg ccaccatttc catgtcgcta tggctgttga tgatttctgt catgatctgg
     2521 cgcatcagtg ccgaatcatc gacagataac accctgattt tgctcatcgt taatccttac
     2581 ttagcgcata caccgtctga ccacgcagcg tgaagcggcg ctcgaggtgg ctaaagtttt
     2641 cagagtgacc cgcaaacagc aatccgtcgg gtttaaggag cggaacaaag cggcgcaaaa
     2701 tctcctgctg ggtagtttga tcgaagtaga tcatgacgtt acgacagaag atcgcatcaa
     2761 acggccccgg cacggtgtac tgtttcgcca gtaaattcag cggggcaaaa tcaacatagt
     2821 tcgccagctc ctgacgcacg cgtaccagcc cttcatgcgg tcccgtccct cgcatgaaat
     2881 accgttgcag ttgctgcggc gtcaggtttt tcaactcttc atggcgatag ataccgcttc
     2941 tggctttttc cagcacttcg gtgtcgatat cactggcaaa cactttccag cgtccgggca
     3001 cggtgcccaa tgtgtcagcc agagtcatcg caatgctgta cggctcttcg ccggtcgaag
     3061 ccgccgcgct ccatacgcga tcctcgccag aacggcgacg tgcgtgatcc gcgagcagag
     3121 ggaaatgatg agcctcacgg aaaaatgccg tcagattcgt ggtcagcgaa ttgataaacg
     3181 cctgccactc accgctgtgc tgattagatt ccagcaagtt cagataatga ccgaaatccg
     3241 tcagtcccag cgaacgcaaa cgacgaacca gtcggttgta aaccatgtcg cgtttatggt
     3301 cagccagaac gatcccggct cgttgataga tcaattgact tatccgccga aaatgcgcgt
     3361 cggaaagcgc caggcgctcg gtcatctgta acaataaaga cgtttgccca cagggcagag
     3421 atgaagtcat agcgccttct caatcacttc aggataccac tggcgcaatt tgtaactgcg
     3481 ccgactcatg tcgtgctact tcatgttctt caagggtaaa taccgccaca cgcgacgaaa
     3541 gatggtcggc ctgattcgcc agttgttccg ttgccaccgc cgcttcttct accagcgagg
     3601 agttctgctg agtcacctga tccatctggc tgacagcctg tgcaacctgc tcaatccccc
     3661 gccgttgttc ttccgacgcc gaagcaattt ctcccataat gtcgttcacg cgggtcaccg
     3721 aactgacaat atcggtcatg gtcgcggcgg cgttattcac cagtttcgaa ccctgctgga
     3781 cacgattgac tgactcttcg atcagccctt tgatctcttt tgctgcctgc gcgctacggc
     3841 tggcaagatt gcgcacttca cctgccacta ccgcaaaacc acgcccctgc tctccggcgc
     3901 gagccgcttc cactgccgca ttcagggcca gaatattggt ctggaaagca attccgtcga
     3961 taacgctgat aatgtcgcca attttttgcg agctggtggc gatctcctgc atggtgtgag
     4021 tcatggtact gacctgaaca ccgcccgcct gcgccgttgt cgcggcattt tttgccagtt
     4081 ccgacgcctg tcgtgcgtta tcggcgtttt gccctaccgt ggcggttaat tgctccatac
     4141 tggcggccgt ttgtgccagc gatgccgcct gctgttcggt acgacttgag agatcgttat
     4201 tgcctgcgac aatctccgcg atcccaatgt gcatctcatg gcttccctta cgcacatcac
     4261 tcaccgtccc acgcaaagcc tgctgcatgg tcttcaggct ggcaaaaatg gcggtgatct
     4321 cattacgacc atataccgca atcggacgcg ccagattacc cgcagcaatg ctgtcaaaat
     4381 ggctaccgat aatggccagt ggttgaacaa tcatcttgcg cgtccaccac agcgcactgc
     4441 tgatgtagat cgccgcaaca ataatcatgc tgataaacac cagcgccgaa atctgatagt
     4501 tacgctggct ttgcgcactg gcggcttcca gcacatggtt gatctccagc tgccaggcct
     4561 caaagttaac gtcaaacgca ttctgtgatc cctgcaccgg cgcagtgagg aaatccgaaa
     4621 gttgattact ttcaagccag gtcgcctgat gttcgagatc gttgtgccag cgggcaaaac
     4681 ttttctccgt ctctttttgc aatgccctga cgtgctcgtt gcccgcagtc atcgccataa
     4741 aacttttaaa cagcgtggtg gattgcgtca gactggcgcg cgccgtcgtc atcaacgttt
     4801 taatgtcatc cgccggatag ctaagcgctg tcagagtgcc tgctttgttc agcgcggtac
     4861 tggcctgtaa cattaccgcc cgagtttgcg ctaatgccgc acgttgctga ttgctctgct
     4921 cgccgtgatt cagtcgttgc aaatcgtcgc gaaatgccca aaaagacatg ccgttactgc
     4981 caatctgcaa gatcccgcag agaatcaaaa ttaaaaacag cgtggtcgaa attcgaatac
     5041 gattaaacat caacgctccc catcaggcgg caatgaccgc gttagtaaaa actcgtcaaa
     5101 atgtttccca gtttggatct tgttcagtaa ttcgcagtcg tggctgtgcc ggtggttgct
     5161 cactggcagg acgggatggt gtttgcggtt tattggtgag tgggctggct gccagacgga
     5221 acgcggaaac cgcttgcgtt aaacgactca cctgttcttc cagcgcagct gcggcggcag
     5281 ctgattcctg caccagcgat gcgttctgtt gcgtgacgcg atccatttcc gaaaccgcca
     5341 atgcgacttg atcgatgcca cggctctgtt catccgatgc cgatgcaatc tcgcccataa
     5401 tgtcagtcac gcgagtgaca gcattgacga tattgttcat tgtttccccg gcgctttcga
     5461 ccagcaccga accggtatca atgcgcgaga cggagtcttc aatgagggct ttgatctctt
     5521 ttgccgcctg ggcgctgcga ctggcaagat tacgcacttc acccgccacc acggcaaaac
     5581 cacggccctg ttcacccgca cgcgcggctt caaccgcggc attcagcgcg aggatattag
     5641 tctggaaggc aataccgtcg ataacgctga taatgtcggc aattttcttc gaactatcgg
     5701 cgatctcatg catcgttttc actacgccat ccaccacttt gccgccgtgc tgggcggtgt
     5761 cggaggcact ttgcgccagt tgcgaggcct ggcgggcgtt atcggcgttt tgcttcactg
     5821 tcgcggtgag ctgctccatg ctggcggcag tttcttccag cgcggatgcc tgctgttcag
     5881 tacgggagga aagatcggtg ttgcccgccg caatttcacg ggtaccggca tagatggcat
     5941 ctgaaccttc gcggacatga gtgacggtgt cagtcaaaga gcgttgcata tgtgaaacgc
     6001 tctgcgccag gtcacccatt tcactgcgcc cgtcaatggt cagggtattc gccaggttac
     6061 caccggcgat ttcgcaaatg tgagcaataa tttttgccag cggagtaagc aacatacggc
     6121 gaatgccgta ccacgccacc agcagaatca ataccaccac cagcgcgata accgccagtt
     6181 gccactgggc aaatcggtaa tcatctgcgt tgtcagtgac gatatcgcga tacagttttt
     6241 cactgctgag ggcgtactga gcaaacgctt cgcccattgc attttgcatt ccctgggttg
     6301 gctgagcgaa ataagctcca gtattgccat aatcaagata atcaatcagt tcagtt
//
LOCUS       DAJTQY010000157         6340 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.157, whole genome shotgun sequence.
ACCESSION   DAJTQY010000157 DAJTQY010000000
VERSION     DAJTQY010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6340
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.157"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            7..438
                     /locus_tag="OO349_003716"
     CDS             7..438
                     /locus_tag="OO349_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HCR5868632.1"
                     /translation="MALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKASKDIQFA
                     VQSGPMLMENGVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKA
                     KLNVEQLLYLGGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            complement(585..2747)
                     /gene="uvrD"
                     /locus_tag="OO349_003717"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(585..2747)
                     /gene="uvrD"
                     /locus_tag="OO349_003717"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HCR5868633.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAEI
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTMHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(2831..3547)
                     /gene="yigB"
                     /locus_tag="OO349_003718"
     CDS             complement(2831..3547)
                     /gene="yigB"
                     /locus_tag="OO349_003718"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HCR5868634.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAELEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(3547..4443)
                     /gene="xerC"
                     /locus_tag="OO349_003719"
     CDS             complement(3547..4443)
                     /gene="xerC"
                     /locus_tag="OO349_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HCR5868635.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDAAMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(4440..5147)
                     /locus_tag="OO349_003720"
     CDS             complement(4440..5147)
                     /locus_tag="OO349_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HCR5868636.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(5144..5968)
                     /gene="dapF"
                     /locus_tag="OO349_003721"
     CDS             complement(5144..5968)
                     /gene="dapF"
                     /locus_tag="OO349_003721"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HCR5868637.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLDEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(6005..6208)
                     /locus_tag="OO349_003722"
     CDS             complement(6005..6208)
                     /locus_tag="OO349_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5868638.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
BASE COUNT         1407 a         1813 c         1648 g         1472 t
ORIGIN      
        1 cagaaggtgg cgttaaatct cgcttcaggt gaagggaatt tctttatccg tcctggcggc
       61 gtgttttatg tcgcgggaga taaagtcggc atcgttcgtc tggatgcttt taaagccagt
      121 aaagatattc agtttgcggt gcagtcagga ccaatgttga tggaaaacgg tgtaattaat
      181 ccgcgtattc atcccaacgt cgcctcacgt aaaattcgta acggtgttgg gattaataaa
      241 catgggaatg ccgtgttttt gttgagccag caggcgacaa atttttatga ttttgcctgt
      301 tatgccaaag cgaaactgaa cgttgagcag ctgctttatc ttggtggcac gatttcacat
      361 atgtatatga aaggcggggc aatcccgtgg caacgctatc cttttgtcac catgatttcc
      421 gtcgagcgga aaggttagaa tgatagcttt gtgtcagata ctaaatatgc tgctgatgcc
      481 ggatgcggcg taaacgcctt atccggccta catgacgttg caatttattg aatctgaatg
      541 attttttagg ccaaataagg tgcgcagcac cgcatccggc aacgttacac cgactccagc
      601 cgggcgtaag ctgccaccag ccatttgatt ccctggccct ggaatgccac ctgcaaacgg
      661 ctatgttcac cgctgccttc catattgacg atggtgcctt caccaaactt agcgtggcgt
      721 acacgctggc cgagcttgta gccgctgtcg ttctcgacca tcggcgtacc catccgctga
      781 tggctgaccg ggcggcttac cgtggcgcgc aggcgcacct cttccacaca ctcttccggc
      841 aactcgccaa taaagcgcga cgggcgatgg taaacctctt tgccatacag acggcgagtt
      901 tccgcgtagg tcagcgtcag tttctgcatc gcgcgggtta cgccaacgta ggccagacgg
      961 cgttcttctt ccagacgccc gccttcatcc agcgacatct ggcttgggaa catgccctct
     1021 tccataccga cgataaacac ctgcgggaac tccagacctt tcgccgagtg catggtcatc
     1081 agttgtaccg catcctgcca ggtatccgcc tgcccttcgc ctgcttccag cgccgcatgg
     1141 gataggaacg cctgcagcgg cattaaatct tcgtcttctt cgttgtagct gaactggcgc
     1201 gttgccgtca ccagttcctc taagttttcg atacgcgtct gacctttttc gcccttctcc
     1261 tgctcgtaca tggtacgcag gccggagtct ttaattaccc ggtcagtctg tacatgcagc
     1321 ggcatatcgg cagtttcctg cgctaaggcg tcgatcagtt ccataaaccg ctgtaaggcg
     1381 ctggcagcac gtccggcgag ggctttttcc tgcaacagtt cacgacatgc ctgccagagt
     1441 gttaactggc gatcgcgcga tgtctgacgt accacgtcca gcgtccggtc accaataccc
     1501 cgcgttggcg tattcaccac acgctcaaag gccgcgtcgt cgttacggtt ggcaatcagg
     1561 cgcagatacg agagcgcatc tttgatttcc tggcgttcga agaagcgcat cccaccgtaa
     1621 atacggtacg gcatactcgc ctgcaacagt gcttcttcca gcacacgcga ctgggcgtta
     1681 ctgcggtaga gaatggcgca ctcggcaagc gccccgccgt tgtcctgcca ggttttgatg
     1741 cggttaacca caaaacgcgc ttcatcgagt tcgttaaaag cgcaatagag ggaaataggc
     1801 tcaccgtccg cgccatcggt ccacagtttt ttacccagac gcccgttatt gttttcaatc
     1861 agggcgttag cggcgctcag aatattgctg gtagagcggt agttttgctc cagacgaata
     1921 atttcggcac cggggaaatc attaaggaaa cgctgaatat tctccacctg cgccccgcgc
     1981 cagccgtaga ttgactggtc gtcatcaccg acgatcatca ctttgccggt gtcgcccgcc
     2041 agcaggcgga tccacgcgta ctgaatgttg ttggtatcct ggaattcgtc caccaggata
     2101 ttggtaaaac gttcgcggta gtgttgtagg atatgcggct tgttaagcca cagttcgtga
     2161 gcgcgcagca gcagctcggc gaagtccacc aggcccgcgc gatcgcacgc ttcctgatac
     2221 gcctgataca ccttctgcca ggtctgctcc accggattac cgtagctttg aatatgatgc
     2281 ggacgcaggc cttcatcttt ctggctgttg atgtaccaca ttgcctggcg cggcggccac
     2341 tgcttctcgt cgaggttcat ggctttgatc agacgcttaa gcaggcgtag ctggtcttcg
     2401 ctatcgagga tctggaaatc ctgcggcaga ttggcgtcca tatggtgcgc acgcagcagg
     2461 cggtgcgcca gcccgtggaa ggtgcctacc cacatgccgc cctggctggt gcccatcagt
     2521 tgtccgatac gatgacgcat ctccgccgcc gctttgttgg taaacgtcac agccataatc
     2581 gagtatggcg agcagttttc cacgctcatc agccaggcga tacgatgcac cagtacgcgc
     2641 gtcttaccac tgcccgcgcc cgccagcacc agaaggttgc tgcgtggcgc ggccaccgct
     2701 tcgcgttgtt tgtcattaag gctgtcgagc aggtaagaaa cgtccattgg caccgccgcg
     2761 taaaaatagg ttgggcggag aagcgcaacg ccctccgcca aaaagctggg tatatataca
     2821 gatttgctga ttatatcagc gaggtcagag atgccaaccg ggaaatttcc agatgtggca
     2881 gtaaacggct gtcccaggtt tgcatcagat cgccattttc cggtctgatc caacaagcct
     2941 gcattccgct gcgaattgcc ccacccacgt cagtggtgag atcgtcccca acatgtaaga
     3001 tctcgccgat cggcacgttg agtttttccg cagccaaaaa gtacatatcg ctgaacggtt
     3061 ttgagcgccc gtgcgggcca gcgcgcagca caaactcaaa ataatccccc aaaccaaaca
     3121 gttccggctg ggcgttaccg ttggtgatcg ccaccagcgg ccatttcttc gccagctgtt
     3181 ttaaggtgtc gtgagtttgc tgcgggacgt cgattcggct gcgccatttg gcaaagttaa
     3241 tcattgctgc gtgtgcgcct gcactggctt cttcggcact cagcccggcg tcgagcatcg
     3301 cttgttcaat cgaacgaaaa cgccaacgtg tcacgtcgtg ataaatctcg agttccgctt
     3361 cccgtaccgc ctggcgcagg cgttgcagat cttcattctg gaagctgcgc agcgccggat
     3421 gataattttg cacaaaggta agcgcctctc gctcggtgcg caaaatcacc ggacggttat
     3481 cgtaaagggt atcatccagg tcaaaggtga gcgccgagat gcgccccaaa ggccggtaaa
     3541 aacgcattat ttcccccgtt tggcgcgtgg atgcgccgca tcgtacaccg aggcaaggtg
     3601 ttgaaaatca agatgagtat agatttgcgt ggtggagagg ttggcatgac ccagtagctc
     3661 ctgcacacca cgaagatcgc cgctcgactc cagcatatgc gtagcgaacg agtgacgtaa
     3721 tttatgtggg tgaacgtgat tattcagccc ttgttttatg ccccattcgg caaagcgttt
     3781 ctgcacatta cgcgcggaga tacgcttgcc cagtttcgac agaaaaagcg cgtcgtcttc
     3841 gctaccaaac aggtcgcgca aatcaagcca gtgctcaatc cacgccacag cgttgcgacc
     3901 aatcggcagg cggcgctctt tgctgccttt ccccatcacc cacacttcgc cggactccag
     3961 gtcgaggtgt ttgatatcca gccccaccag ctcggaaaga cgcagacccg cgccgtacat
     4021 cacttccagc attgcacggt cgcgtacagc gaggggatcg ttgatatcaa tatccagcag
     4081 ccgattcata tcgtcgacgt cgatattttt cggcagatga cgcggcgctt tcggtgccga
     4141 aactcctttc gccgggttag ctttgagttc gttctggctg accagccagt caaaaaagct
     4201 acgtagcgca gaaagccgta acgccagact tgctgctccc agccctttac ggcgactgcg
     4261 tacagcaaaa ttgcgtacca tcgctgcatc gcattgttgc cagctttgca ggccgttttc
     4321 gctggcaaaa ttgatgatcg cctcaagctg acgctggtag ttaagtaggg ttatcgggct
     4381 aagctgacgc tccacgctca gataacgtag gtagcgttct acatcggtgt gtaaatcggt
     4441 catacgcgtt caatccaacg ctccagaagc tccggcaaca tcagcgcaat ttcatgaagt
     4501 aactgcgttc cctgcccttg ttgatagtga ctggcatcgc gactggtaaa cagcacaaca
     4561 cccaaatcag catcgcttcc cagcatcgac atcgccaccg atcccaccgc tttcgcttcc
     4621 ggtagcacca ccagcagctc cggtccgtta agcggcccaa gatagtgctg ttcctgcccc
     4681 aaacgctgaa tacgcagcgg ttcgaaagac tgacggctta atgccagatg agtgtggttc
     4741 gacggcgcac ctaagcgcca gcgatctgga aacaggcgca gactcgcacc agctaggccg
     4801 agatcgcgcg cccagcggtg aaagcgcatc agcatatcgt cgagactgct ggcggcggtg
     4861 agactgcgct gcaggtagag taggcgataa aacagccctt cgttggcgat agcctgctcc
     4921 atcaacaacg ccatgttctc ttccagaaca tgaatgtgat tacgtgcgcg ggccatgtgc
     4981 cactcgacca acgaaacggt gccgcgtacc ggatgcggca cacgtatcgc ttctactgcg
     5041 cgcgcattgc ggataaaaaa ctcaggattt ttaatcagat aatcgacaac cgcccggtca
     5101 tcaagctccg tgagtgtttc ctgcagttct tcccctggtt gcttcataga tgaataaatc
     5161 catcgtagac atgtaccgcc gggccagtca tatataacgg gtgacccgga cctttccagg
     5221 cgatatcaag acgaccgccg gggagttcca cgcgtacttc ttcgtccagc aaaccttgct
     5281 gaatccctac cgccaccgcc gcacacgcgc cgctgccgca ggcctgggtt tctcctgccc
     5341 cacgctcata aacgcgtaaa cgaatatgct cgcgcttaac cacttgcata aaaccgatat
     5401 tggcgcgctc cggaaaacgc tcgtgacttt ccagaacagg accaagcgtt tctaccgccg
     5461 cggtatcgac atcatcgacc tgaatcacgc aatgcggatt tcccatcgac accacgccgc
     5521 ataagattgt ctgctcggcg gcgcgcataa tataggtctt ttccgctttg ttagcgcgaa
     5581 acggcacggc ggaaggttcg aagttgggtt cgcccatatt tacgcggacc agatcatcat
     5641 cggtgacggt cagaaccatc cgcccgttgg cggtgctgac gcggatatca cgcttattgg
     5701 tcagtccttt cagacgcaca aaacgggcaa agcagcgcgc accgttaccg cactgcgcca
     5761 cttcactgcc atcagcattg aaaatgcgat agtgaaaatc cagttcagga tcatacggcg
     5821 gctcaaccac cagcagctgg tcaaacccta cccccaggtg ccgatccgcc aggcgacgaa
     5881 tcagctccgg tgaaaaaaag acattctgcg ttaccgcgtc gacgaccata aaatcgttgc
     5941 caaggccatg cattttcgag aactgcatca tttactccaa tcacgcgggt acagaaactg
     6001 acttttagta attcacctgg gatggaccat cgccagtggc gcgatcgttt ttatccggca
     6061 ccgtggattg cgtttgcgtc tctaccggtt tggtcggcgg cggtgcgttt ttatctgcag
     6121 gcgggaaata gagcggacct ttcagaccgc agcccgtcag gctgaagaga gtaagtaata
     6181 cagtgagtgc cttaaacacg tttttcatta tggattgcct gtaaagttca tcgctttctg
     6241 ctttctgctt tctatcatcg caggagaagg cgtaaaagca agcgtttagc gactctttat
     6301 gaagtctgaa aagtgaggaa atgctgcgag tataacgctt
//
LOCUS       DAJTQY010000158         6270 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.158, whole genome shotgun sequence.
ACCESSION   DAJTQY010000158 DAJTQY010000000
VERSION     DAJTQY010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6270
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.158"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(<1..666)
                     /locus_tag="OO349_003723"
     CDS             complement(<1..666)
                     /locus_tag="OO349_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol phosphorylceramide synthase"
                     /protein_id="HCR5868639.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDLNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSSTFEQRRLVHRLILATVMACCG
                     FFLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAVR
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKLDQRRIK
                     IALP"
     gene            complement(656..2413)
                     /locus_tag="OO349_003724"
     CDS             complement(656..2413)
                     /locus_tag="OO349_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HCR5868640.1"
                     /translation="MENSRIPGEHFFTTSDNTALYYRHWPTLQPGAKKVIVLFHRGHE
                     HSGRLQHLVDELAMPDTAFYAWDARGHGKNSGPRGYSPSLMRSVQDVDEFVRFAVSDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIVSDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFICRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGA
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQE
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLELIAGVLTSHKDGKPWVMRVRSQGEMDSLVRNAGFDKCT
                     QRIDVWGIFTVSMAVRRDN"
     gene            complement(2429..3313)
                     /locus_tag="OO349_003725"
     CDS             complement(2429..3313)
                     /locus_tag="OO349_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005142702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCR5868641.1"
                     /translation="MSGNTFRVVNFSKLINRFLLWRLPERKGDEVTLRIRTWWGIVIC
                     FSLVISGPRWMTLTFFTLISFLALKEYCTLISVHFSRWLYWVIPLNYLLIGFNCFELF
                     LLFIPLTGFLILATWRVFVGDPSGFQHTVSAIFWGWIMTVFALSHAAWLLMLPTINIQ
                     GGALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLLGGVITTMIASLI
                     IGPLLTPLNTLQALLAGLLIGISGFCGDVVMSAIKRDIGVKDSGKLLLGHGGLLDRID
                     SLIFTAPVFFYFIRYCCY"
     gene            complement(3324..3929)
                     /locus_tag="OO349_003726"
     CDS             complement(3324..3929)
                     /locus_tag="OO349_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCR5868642.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLTALALSLFTGL
                     LLVLVAQPILFLLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGVRSYAGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILFICTAINRCRSVLLMSAER"
     gene            complement(4100..>6270)
                     /locus_tag="OO349_003727"
     CDS             complement(4100..>6270)
                     /locus_tag="OO349_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
                     /protein_id="HCR5868643.1"
                     /translation="KQQVEQAINSSVNLVPFGLSASDWKVHRGDLVVEGNIESNQKLI
                     VLGNLTVKGNISTFSLSNPWVILGNVTATNIVTDSPLLITGSINASGLVFIDSYYDNP
                     STIKGSINARGVFINDIIAPVVASSTNSEFMVRASDKNDTENVKKALMIINPDAYYWG
                     LINDEDALKEIFKRSNIRMAGNVCNQMKKEALFRPKPSPELVQELQMLDEGNVAAFEG
                     RDIATFDLAIMRTLPRLKGISANLRKQLINSNDEQTIESMARYMPDNEILELTDQQLG
                     YQPVVLGLLDREPLSVEIMTRMSRLPDGVGPLNLALRENLPLDIVMTLAKRDWDMIIQ
                     ELYKDAWLLPESIIDGYIRSDDSSIRQVGAGGQLTYNQAMQLANDSSNNVVTSLAFKL
                     AEMKHHGQLLRMTPQESDKVAAYLYQKFENDDDLIRVLFLALPDNLQFNFVKRMEKKS
                     PAYFCCRDMQVIHSDAALQRLLTRFNDPEGWSNLAKNQYLSTSMKQKIWQRALSHRKN
                     NPKADSDAYETSADMILSELISHGEVDDQMLLNAAALIRLEDWDFLESALVSWDNLPA
                     VVLKELQQNTPRNDIWAKFFLRQENSSRAQVDEALRVYYALDPDALAQLDVLAKQPDR
                     IWWSTLAKSNLTFFKFGALSNRHTPSAALAAEIDPEWWIVAMNNPRFPVDVLKARLKR
                     DPLLALELVNPELDLVRQLALNGKTRAIREQAMRKLDELY"
BASE COUNT         1836 a         1568 c         1316 g         1550 t
ORIGIN      
        1 tggcagtgct attttgattc gacgttgatc aagtttctga taattccaac gacggtcgac
       61 gggcaccatc cagtcaatca acatacctac cgccagccct gtgatgacat caataaaatg
      121 atgctgccat gtcgtcagcg tcgaaatggc gatgagtaaa aaccatccgc cacagacttt
      181 acgccacctc acagccagat gctgacgaaa gtgacgccaa agtagccagc agagaataat
      241 atgcagcgaa ggagactggt tataaggcag atcaaacagt tcaagttgcg aaaatagcca
      301 tcctgtcacc ccactcactt caggacggat aaaactaaac ttcagcgggt agagaaaaaa
      361 accgcagcag gccattaccg ttgccagaat aagccggtgg acaagtcggc gttgttcgaa
      421 tgtcgagcta caaacgaaca gcgaaaatcc atataaaaga tccagactcc agtaaggaac
      481 aatagtccag ggaagaaaag ggatcgccgt ttcccaaccg aatacctgac tggggatatc
      541 atggctgtta aggtcctgaa ccgcggtgaa ctgattaaga gatccatagg tgaagaagaa
      601 aaacggggcc agcaacaata accagccagc gccttgtagc aatacgttcc gacgttcagt
      661 tatcacgacg caccgccatc gaaaccgtaa aaatgcccca cacatcaatc cgttgagtgc
      721 atttatcaaa tccggcatta cgcacgagcg aatccatctc cccttgcgaa cgcacgcgca
      781 ttacccacgg tttaccatct ttatgactgg ttaacacccc ggcaatcagc tccagttgtg
      841 ggtgccacgg ctgcccggtg tagatgagaa tgccacccgg ttcgatggca ttggctagac
      901 ctgcgagtga gttttttacc tgctcatttt cgggaaaaag ctcatatagg ccagagacaa
      961 tcgccagcgt aggccgcgga gttaacgcgc tgagttcctc cgggttaaac gcatcgccct
     1021 gttcaaaacg cacccgccca gacattcccc gttgggcaat catctcttgc ccctgtgcaa
     1081 catttaactc actgtaatca cgtaacaaaa tatcgcttac ggcaggctcg tttgccagcg
     1141 catccagtac atagcgtcca tgccctgcgg caatgtcaac cacgcggacg gctaaacctt
     1201 tggcgtggag atcggcaacg gcttgtttaa tcaatatttg caaatgggtt ttgcgctggc
     1261 gaataccgcg ccatccaaca ctgttcaggt agattttgtc gattaaacgc ccgaatgcgt
     1321 tactgccttg cggttgattt tgatagacat aatccaggga actgcctgaa tcaaagccgg
     1381 tactcattcc gagatgcagg cccgcagagt gcgcaccgaa caatttcatc gcctttcgca
     1441 taaagcgata cgctaaatca accggcgata atggcacggg tccaccagaa aggagccgcc
     1501 atcgatccgc tgatggtcct gtgcggtctt catgctgata atcaaatttt tgcgatttgt
     1561 tagcatataa gcggcagata aagctttgca ttttttcaaa tgcctgcgcc ctgttctctt
     1621 cacccaatgt gtcgtgataa aagcctggca gcagatgcag ctctttcaga gggctacgta
     1681 atctctgata aaaatcaatc tgcggttggc gatgcaccac atagtcatca cctgatatca
     1741 gaagttgcgt ggggagcgta atcgccgccg catcgctaac aatacgttca gacgttttat
     1801 agagatcgag caagatattg acggcaatcg cccgtgtgat cagcggatca ttattgaaac
     1861 tcgccacccg ttgccgatcg tgggtcaaat agcgcccttt cacataggaa ttaataaaaa
     1921 atagaccacg cagacgatgc cataacgcca gcgcaggacg tgccagcggc acatacaatt
     1981 taaccttaaa ggccggagaa gccagcacca gcccacgaat tgcaggtgca taatcatgaa
     2041 cccatgtggc aacaagcact gcgccgacgc tttgcgcgat cactaccacc tcttccagtc
     2101 cgacctggct gtcgctgaca gcaaaacgga caaattcatc gacatcctgc actgagcgca
     2161 taagagacgg gctataacca cgcggccccg aattttttcc atgcccccgg gcatcccagg
     2221 cataaaaagc agtatcaggc atcgccagtt catcaacgag atgttgtaga cgaccagaat
     2281 gttcatgccc gcgatgaaat aagacgatga cctttttcgc ccctggctgt aaagtgggcc
     2341 agtgccgata atacaacgct gtattatcac tggtggtaaa aaaatgttcc ccagggatgc
     2401 gtgaattttc cattttctct attttccttc agtaacagca gtagcgaata aaataaaaaa
     2461 ataccggagc ggtgaaaatt aatgagtcaa tcctgtcaag gagtccgcca tgtcctagta
     2521 atagttttcc actatcttta acaccaatat ctcgtttgat ggctgacatc actacatcgc
     2581 cgcaaaaacc actaatacca attaacaaac ccgctaataa tgcctgtaat gtatttagcg
     2641 gtgtcagcaa cggtccaata atcagtgacg caatcatggt ggtgattacg ccccccagca
     2701 gaccttccag cgtttttccc gggctgactt tggggaccac ttttcttctg ccgcaggatt
     2761 tcccccataa atactgtgca atatcgtttg actcggttaa cgccaaaaga aataacacca
     2821 gtaacgcccc gccctggata tttatggttg gcaacattaa tagccaggcg gcatgactca
     2881 aggcaaaaac ggtcattatc catccccaaa aaatggcgct cacggtatgc tgaaaaccgg
     2941 aggggtcacc cacaaaaact cgccaggtgg ctaatatcag aaacccagtc aaaggtataa
     3001 ataacagaaa cagctcaaag caattaaaac cgataagcaa atagttaaga ggaataaccc
     3061 aatataacca acgcgaaaaa tgtacggata taagcgtaca atattctttc aatgccagaa
     3121 aacttatcag cgtaaaaaac gtcaacgtca tccagcgtgg gcctgaaatc accagtgaaa
     3181 aacaaatgac gatgccccac caggtacgaa tacgtaatgt aacttcatcc ccttttctct
     3241 caggtaaccg ccatagcaga aaacggttta tcagcttgct aaaattaaca acgcgaaaag
     3301 tgttgccaga gatttttcca gcattatctt tcagcgctca taagaagcac actccgacaa
     3361 cgattaatcg cagtacagat aaacagaatt gaagcaatgc tccataaaag attattccat
     3421 tgcatccact gtggataaat ggcaacggcc agaccccaca gaccaaatat taacgcgcga
     3481 tcacttttgc caaatggacc ggcataactg cgaacaccgt taatcgtctg cgcgagtaaa
     3541 ccgcaaaact cggtcaatat ggtgcaaaat aacatgagta tcacgagtga tgcgttactt
     3601 tccggtaaaa ataaaaacgg taaatagagc gcaatatcgg aaataacatc gccagtctca
     3661 ttcaaaatcg cccccagacg tgtttgctga ttgcactcac gcgccaacat gccatccagc
     3721 gcattgagcg ccatacggat aaaaagcacg atgggcaata gcaaaaagag gatgggttgt
     3781 gccaccaaca ccaacagcaa tccggtaaaa agagaaagcg ccagtgccgt aagagtgatg
     3841 tgattcgctg taacgtggtg cttataaagc caaaacatcg tcggccttaa cagcgactga
     3901 aagagcggtt ttatctggta gagcgtcatt atcaatcctt gataattcgg tttgcgctat
     3961 ggtgcatcaa ttcccctgac ggagatatcg caacacgccg cttcattaaa aattcagaat
     4021 atatcaaggt attgggggct cactgtcagg aatgattatc cccaataaat caagtagtta
     4081 aaaattatta cgctgttatt caatacaact catcaagttt cctcatcgct tgttcccgta
     4141 ttgcgcgcgt cttaccgtta agcgccagct ggcggactaa atccagttca gggttaacaa
     4201 gctctaacgc cagcaaagga tcacgtttca gcctcgcctt caatacatca actggaaagc
     4261 ggggattatt catcgccaca atccaccact cgggatcaat ttctgccgcc agtgccgcag
     4321 agggggtgtg gcggttgcta agtgcgccga acttgaaaaa tgtgagattg cttttcgcca
     4381 gtgtactcca ccatatacga tccggttgtt ttgccagtac atccagttgt gctaacgcat
     4441 cggggtcgag tgcataataa acacgtaatg cttcatcaac ctgcgcacgg gagctatttt
     4501 cctgcctcag aaaaaacttc gcccaaatat cattgcgtgg cgtgttttgc tgtaattcct
     4561 tgagaacaac agcgggtaaa ttatcccaac taactaatgc actctctaaa aagtcccagt
     4621 cttccaaacg tatcagggcg gcggcattta atagcatctg gtcatcaact tcgccgtggc
     4681 taatcagctc agacaaaatc atatcagcgc tggtttcata tgcatctgaa tctgctttcg
     4741 gattattttt ccgatgcgat aaagcacgtt gccaaatttt ttgtttcatc gacgtgctca
     4801 gatactgatt tttcgccaga ttactccacc cctcaggatc gttgaaacgt gtcaataacc
     4861 gttgtaaagc agcgtcagag tgaattacct gcatatcccg acagcaaaag taggcagggg
     4921 attttttctc catcctttta acaaaattaa actgtaagtt atctggcaat gctaaaaata
     4981 acacacgtat tagatcatcg tcattctcga atttttggta taaataggct gcaactttgt
     5041 cactctcttg tggcgtcatc cgcaataatt gaccatggtg tttcatctct gccagcttga
     5101 acgctaagct tgtgacaaca ttgtttgatg aatcgttcgc caactgcatt gcctgattgt
     5161 aagtgagttg tcctccagca ccgacctgac gaatagagga atcatcactg cggatatagc
     5221 catcaataat ggattcgggc aataaccacg catctttata aagctcctga atgatcatat
     5281 cccaatctct tttcgccaga gtcatgacga tatccagagg gagattttca cgtagcgcaa
     5341 gattcaacgg accgacacca tcaggtagac gactcattcg cgtcattatt tcgacagaga
     5401 gcggttcacg gtcgagcaac cccagaacaa caggctggta acccaattgc tgatcggtca
     5461 actccagaat ctcattgtca ggcatatatc tcgccatact ttcaatcgtt tgttcatcat
     5521 tgctattaat aagttgtttg cgtaggttag ctgaaattcc tttaagcctt gggagagttc
     5581 gcattatggc aagatcaaat gtcgcaatat ctcgtccttc aaatgcagca acgttgcctt
     5641 catccagcat ttgcaattcc tgcactaact caggagaagg cttagggcga aacagcgctt
     5701 cttttttcat ctgattacag acattccctg ccatgcgaat attgcttcgc ttaaaaattt
     5761 ctttcagagc atcttcatca tttattagac cccaataata tgcatcagga tttattatca
     5821 tcagcgcttt tttgacattt tcagtgtcat ttttgtcact cgcacggacc atgaattcac
     5881 tatttgtcga agacgcaaca acaggtgcaa ttatgtcatt gataaatacc ccacgcgcat
     5941 taatactccc cttaatcgta gacggattat cgtaatatga gtcgataaat accagtccac
     6001 ttgcatttat cgaaccggta attaacagtg gtgaatccgt aactatattc gttgccgtca
     6061 cgttaccgag aataacccat ggattagaaa gagagaaagt ggaaatatta cctttgactg
     6121 tcaaattgcc aagaacaatc aatttttgat tactctctat attaccttct accactaaat
     6181 cgccacgatg cactttccag tccgatgcag ataaaccgaa ggggacaaga ttcacgctac
     6241 tgttgatagc ttgttcaact tgttgtttat
//
LOCUS       DAJTQY010000159         6255 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.159, whole genome shotgun sequence.
ACCESSION   DAJTQY010000159 DAJTQY010000000
VERSION     DAJTQY010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6255
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.159"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            376..1200
                     /gene="gadX"
                     /locus_tag="OO349_003728"
     CDS             376..1200
                     /gene="gadX"
                     /locus_tag="OO349_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HCR5868644.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTNRSAEGIRL"
     gene            1568..2296
                     /gene="gadW"
                     /locus_tag="OO349_003729"
     CDS             1568..2296
                     /gene="gadW"
                     /locus_tag="OO349_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HCR5868645.1"
                     /translation="MTHVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2659..5772)
                     /gene="mdtF"
                     /locus_tag="OO349_003730"
                     /pseudo
     CDS             complement(2659..5772)
                     /gene="mdtF"
                     /locus_tag="OO349_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417971.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
     gene            complement(5797..6054)
                     /locus_tag="OO349_003731"
                     /pseudo
     CDS             complement(5797..6054)
                     /locus_tag="OO349_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
BASE COUNT         1657 a         1456 c         1477 g         1665 t
ORIGIN      
        1 acctgataac ataacgttgt aaaaaccgaa tgcccagcct ttaaaaaaac agctgggcat
       61 tcggttgctt attaatcgca ataatatatt ggctgtctat tcatcgtgtt gataagatat
      121 atgttatatg aatgtttatt aaatgtagcg attatcttta atgtcacgaa aagggattat
      181 ttgcttgcta ttaatttcac tgcgttctta aacacgtttt tgtattataa atccagtcat
      241 cctgcccgta gaacatcaca cattatcatc ctgttctccc gcttcgttta aatttattta
      301 tcaatcaatt tgacttaaga gggcggcgtg ctacattaat taacagtaat atgtttatgt
      361 aatattaagt caactatgca atcattacat gggaattgtc taattgcgta cgcaagacat
      421 aaatatattc tcaccatggt taatggtgaa tatcgctatt ttaatggcgg tgacctggtt
      481 tttgcggatg caagccaaat tcgagtagat aagtgtgttg aaaattttgt attagtatca
      541 agggatacgc tttcattatt tctgccgatg ctcaaggagg aggcattaaa tcttcatgca
      601 cataaaaaaa tttcttcatt actcgttcat cactgtagca gagatattcc tgtttttcag
      661 gaagttgcgc aactatcgca gaataagaat cttcgctatg cagaaatgct acgtaaaaga
      721 gcattaatct ttgcgttgtt atctgttttt cttgaggatg agcactttat accgctgctt
      781 ttgaacgttt tgcaaccgaa catgcgaaca cgagtttgta cggttatcaa taataatatc
      841 gcccatgagt ggacactagc ccgaatcgcc agcgagctgt tgatgagtcc aagcctgtta
      901 aagaaaaaat tgcgcgaaga agagacatca tattcacagt tgcttactga gtgtagaatg
      961 caacgtgctt tgcaacttat tgttatacat ggcttttcaa ttaagcgagt cgcagtgtcc
     1021 tgtggatatc acagcgtgtc gtatttcatt tacgtctttc gaaattatta tgggatgacg
     1081 cccacagagt atcaggagcg atcggcgcag ggattgccga accgtgactc ggcggcaagt
     1141 attgttgcgc aagggaattt ttacggcact aaccgttctg cggaaggaat aagattatag
     1201 agttttactc agacataaaa aaaacccggc ataggggacc gggaagagga tagtctgccg
     1261 tctccagact aataaaccgt tataacactc cctgttggca cgggaaactt tgtgctctca
     1321 gtaagttaaa tataactttt actggaaata agatcagcca tttttttata aacataagct
     1381 atacgctgtg cgaaaatata ttcgtgctgc atttacttat tatcaattaa ctgttatgca
     1441 aaactacttt gtggataaat tttggtccta ccaaatctgg cagtttttgc gctaagaaac
     1501 agtctgtcat catttcatta gtatactgaa attgaaataa tcgcagtatg aaatataagg
     1561 gataatcatg actcatgtct gctcggtgat cctcattcgt cgttcattcg atatttatca
     1621 tgaacagcaa aaaatatcgc tgcataacga gagtatcctg ctgctggaga aaaatttggc
     1681 agacgatttt gcgttttgtt caccggatac gcgacgactg gatatcgatg agctgacagt
     1741 ttgccattac ttacaaaata ttcgtcagct accacgcaat ttagggttac atagcaaaga
     1801 ccgtctgtta attaaccagt caccccccat gccgctggtg acggcgattt ttgatagctt
     1861 caatgaatcc ggggtaaatt caccgatact gagcaatatg ctctaccttt cctgtttatc
     1921 gatgttttct cataagaaag aactgatccc cttacttttc aatagcatca gcactgtttc
     1981 aggaaaagtt gaacgcctta ttagctttga tatcgccaaa cgttggtatc tgcgcgatat
     2041 cgcggaaaga atgtatacca gcgagagtct catcaaaaaa aagttgcagg atgaaaatac
     2101 ctgtttcagt aaaatattac tcgcttccag gatgtcgatg gccagacgat tactcgagtt
     2161 acgtcaaatt cctctgcata ctattgcgga aaaatgtggc tatagcagta cgtcgtactt
     2221 tataaacaca tttcgacaat attatggtgt aacgccacat cagtttgcgc aacattcgcc
     2281 aggtaccttt tcctgacata ttttgcattt gaatattggt caggatctca cacctgcttc
     2341 atgtgaaact cttccctgat gatttctgcc gggctaccgg ctagttctct ttcgcagttt
     2401 gccaggcggt ataggtattt ttaggaggaa aagactcggc atgtttggga ttattaagct
     2461 gacaattcat acgattacgg gaatgtgggt cagtattgtg ttgttcaaat tgatgacgaa
     2521 tggctggagt ggtttctact ttcagtgctg tgtactaagc ctggtattcc tgacggttag
     2581 ctggttgttg agtggtgaat ggttagcagg aaagagtaag gctgaacctt cacgttcaac
     2641 cttactctca tttacacgtt acgctttttt aaagcgggca aagagatgtt ccactacaac
     2701 gaaaaagacc ggaacgaagt aaattgccag cactgttgcg gcaaacatcc cgcccatcac
     2761 gccggtacct accgcgtttt gcgcgccaga accggcacca tgactgataa ccagcggcag
     2821 cacgccgaga ataaaggcca gagaggtcat caggattggg cgtaaacgca tccgcgccgc
     2881 ttcgatgatt gcctctatcg gcgttttccc ttctttctgc atcatctcaa cggcaaattc
     2941 gacaatcagg atggcgttct tggcggaaag cccgatggtg gtcagcaaac caacctggaa
     3001 gtagacgtca ttacttaagc cgcgcagatc ggtggccagt aatgcgccaa cgacgcctaa
     3061 cggaacaacc aacatcaccg agaacggaat tgaccagctc tcatagagtg cggcgagggc
     3121 gaggaacacc acgaccagtg aaatggcata cagtgcagga gcctggtttg aggataacgc
     3181 ttcctgatac gacagtccgg tccatgagta gccgacgcct gccggaagtt tagcgaccag
     3241 gtctgccata aatttcatag cgtcaccggt acttttcccg gccgccgctt cacctaaaat
     3301 ctccattgac gggatgccgt tgtagcgttc caatcgcggt gaaccatagg tccattcggt
     3361 agacgagtag gcagaaagcg gtgccatcgt gccagaggcg ttgcgtacat accattggtt
     3421 gatgttatcc ggcaacatac ggaacggcgt gcctgcctgg acatacactt ttttcacccg
     3481 cccctggttg aggaagtcgt tcacgtagct gctgccgaag gcggtggaaa ttgtctggtt
     3541 gatatcagac agcgccacac ccattgcctc agctttcgcg gcgttgacgt tcactttgaa
     3601 catcggcgta tcttccaggc cgttcgggcg taccccggtg acctggtcag gcgattgcgc
     3661 tgccagtgat aacagctcgt ttcgtgccta ggttagtttt tcgtgcccca ggttaccgtt
     3721 gtccagcagt tccatatcaa aacctgacgc ggtacccagt tcagccaccg cgggtaagtt
     3781 gaacgggaag acgacggctt tattgatact gcttaacgca atcattgccc gctgaatgat
     3841 cgcggtaacc gagttttcct caccgacacg ttcagaccac ggcttgagac tgataaacgc
     3901 caggccgttg ttttgcccct ggccgctgaa gccaaagccg ccaacggtaa acaccgactg
     3961 aacattatct ttctcttttg taagataata atccgtcacc tgttgcagta ctttcgtggt
     4021 gttaaccatg gtggcaccgg aaggtaactg cgcggtggtc ataaataccc cctgatcctc
     4081 ttctggtaag aaagaggtcg gtgtacgcag gaacagcacc gccatcccgg cgcaaatcag
     4141 caggtagacc accatgtagc gaccggtaca acgcaacagc gagcgggtgc tatcggtata
     4201 gtgctgagtt gatttttcaa acagcgtgtt gaagcgtgcg aacagggcgt taggtttgtg
     4261 accgccttcc ggcgtggctt tcagaatggt ggcgcacagg gcaggggtca ggctcattgc
     4321 cacaaatact gaaagccgca tggaggagat cagcgtgatg gagaactggc ggtagatctc
     4381 cccggttgca ccgctcataa aggccatcgg cataaacact gcagaaagaa caacggcaat
     4441 cccgaccagc gcacgttgga tctgccccat cgatttatgc gtcgcttcct tcggcggtag
     4501 cttatcttcc gcaatgacac gctcgacgtt ctccaccacc acgatggcgt catccaccag
     4561 taaccctatc gccagcacca tcccgaacat agtcaacgtg ttgatggtga aaccgaccgc
     4621 cgacaagatc gcaaacgtcc cgagaataac caccggtacg gcaattgtcg ggatgatggt
     4681 ggcacggaaa ttctgcaaaa acagataaat gaccaggaag acgaggatga tagcctcaac
     4741 cagtgttttg aaaacttcct gaatagaaat ttcgataaac ggcgtggtgt cgtaaggata
     4801 aaccgtcttc agacttgcgg ggaaataggc tgataagcgg ttcagctcct ctttgactgc
     4861 ccgcgaggta tccagggcgt ttgctccggc agccagtttg atggcgatcc cggcagcagg
     4921 tttgccgtta tagcgcgcca cggtggaata atcttccgcc ccaagttcga cgcgggcgac
     4981 atcacgcagc agcacttgcg aaccatcttg ctgaactttc aacaggattt tgccaaattc
     5041 ttccggcgtt tgcagacgcg tctgcacaat gatcgaggcg tttagttgct ggtctgccgc
     5101 ctgtggcatg ccacccagtt gaccaccaga aatctggttg ttttgcacct taatctggga
     5161 aataacatcg gaaggtacca ggttatattt attgagtttt tgcgggtcca gccagatacg
     5221 catggcgtac tcggaaccaa agagctgtac gctaccaacg cccgcggtac ggcttagcgg
     5281 gtctttgata ttagacgcta catagtccgc gatatcgtac tggttgaggc tgccgttatc
     5341 agaaataaac gccgctacca tcaggatatt actgctcgac ttatcgacgc taatcccctg
     5401 ttgctgcacc gcttcaggta atgaaggcat agcgagttgc agtttatttt gcacctgaac
     5461 ctgtgcgata tcaggagatg tcccagtctc gaaggtcaga gtgatagagg cattacccgc
     5521 cgcatcactg gttgaagaca tgtacatcag gccatcaagc ccattcatat tttgctcaat
     5581 cacctgagtg accgagtctt ctaccgtttg cgcatcggca cccggatagg tagcgctgac
     5641 ggtaatggtc ggtggcgcaa tctgcggata ctgcgcaacc ggtaagttca tgatcgccag
     5701 accacctgca agcatcataa taatggcaag tacccaggca aaaaccgggc gatcaataaa
     5761 atagttagcc atgaaagtcc ccttaagcct gcaacgttat tgtttcgatt cggtgctggc
     5821 gttttcctgg ctggaggaaa ttgctcgtgc tttgataccc ggacgaatgc gttgcaaacc
     5881 ggaaacgatc acccgatcgc cagcctgcaa gccagaggtg acaacccact ggtcgccgat
     5941 ggctttgctg gcttcaattt cgcgtagctg cacgacatcg tctttatcca gaatgagcgc
     6001 cgttgcttta ccctgggcgt tgtgggtgac gccttcctgc ggcaccagta atataaagcg
     6061 cgcaaaatta ttcaagatat ttttgaaaat cattcaagat aggctatcgc gtgtgatagc
     6121 aaaaacattc aagataaaaa tgcttgatta ttaagcaatc aggaatggga gtattaagtt
     6181 tcgtgtttcc agtaaaacca gagagtattg aaatagctat cacctaactt tatcaggcga
     6241 tagctggtag atgat
//
LOCUS       DAJTQY010000160         6252 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.160, whole genome shotgun sequence.
ACCESSION   DAJTQY010000160 DAJTQY010000000
VERSION     DAJTQY010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6252
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.160"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(<1..263)
                     /locus_tag="OO349_003732"
     CDS             complement(<1..263)
                     /locus_tag="OO349_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HCR5868646.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITVTAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFV"
     gene            484..912
                     /gene="uspG"
                     /locus_tag="OO349_003733"
     CDS             484..912
                     /gene="uspG"
                     /locus_tag="OO349_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HCR5868647.1"
                     /translation="MYKAIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(1033..2598)
                     /gene="ahpF"
                     /locus_tag="OO349_003734"
     CDS             complement(1033..2598)
                     /gene="ahpF"
                     /locus_tag="OO349_003734"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HCR5868648.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGNIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(2843..3406)
                     /gene="ahpC"
                     /locus_tag="OO349_003735"
     CDS             complement(2843..3406)
                     /gene="ahpC"
                     /locus_tag="OO349_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HCR5868649.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            3778..4524
                     /gene="dsbG"
                     /locus_tag="OO349_003736"
     CDS             3778..4524
                     /gene="dsbG"
                     /locus_tag="OO349_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HCR5868650.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFSDPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGCKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            4734..5636
                     /locus_tag="OO349_003737"
     CDS             4734..5636
                     /locus_tag="OO349_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5868651.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLIAKSAGVDDIQMEIDEK
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNYHRDFLNLKEIKLEHPLPSIKLY
                     ISYNKSSLNNLVFSRFIDRLNESF"
     gene            5783..5986
                     /locus_tag="OO349_003738"
                     /pseudo
     CDS             5783..5986
                     /locus_tag="OO349_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
     gene            complement(6005..>6252)
                     /locus_tag="OO349_003739"
     CDS             complement(6005..>6252)
                     /locus_tag="OO349_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR5868652.1"
                     /translation="GLEGSMSRRGNCHDNAVAESFFQLLKRERIKKKIYGTREEARSD
                     IFDYIEMFYNSKRRHGSSEQMSPTEYENQYYQRLGSV"
BASE COUNT         1673 a         1512 c         1397 g         1670 t
ORIGIN      
        1 atgacgaacg gaattaccac ccggtcgcct ttttgcaaat tttttacgtc cttcccggtt
       61 tcaacgactt cccccataaa ttcatgacca aaaatatcgc catgtttaac ctggggtatt
      121 ttgcctcgat aaagatggag gtcagagcca cagatcgccg ttaccgtaat acgcagaata
      181 atatcatctg cctgttcaat gcccggatcg ggaacatttt ctacctgaac gtgatgtggg
      241 ccgtgataag tcaatgcttt catgcaacct ccagagacag ttcaaaaaag agtcagtctt
      301 taagggtagc tatcacagcg ggaaaacggg cggtaccgca ggttttggga gaattcctag
      361 tgttcagtga gaatatatta atcacagaaa ccgttcgtaa ggaaaaagaa ccagacaaat
      421 atcagggata aatattataa tcatggagaa tgatttgttc atgattaaca gggagaaagg
      481 gttatgtata aggcaatcat tatgccagtt gatgtatttg aaatggaatt gagcgacaaa
      541 gctgttcgcc acgctgaatt cctcgcccag gatgacggag ttattcatct acttcacgta
      601 ctacccgggt cagccagcct gagcctgcac cgttttgccg ctgatgtgcg tcgttttgaa
      661 gagcatctgc aacatgaagc agaagaacgt ctgcaaacga tggtcagcca cttcaccatc
      721 gatccttccc gcattaaaca acatgtccgt tttggtagcg tgcgggatga agtcaatgag
      781 ttggcagaag aactgggggc tgatgttgta gttatcggtt ctcgcaaccc atcgatttcg
      841 acccatctgt taggttctaa cgcctcgagc gtgatccgcc acgccaatct gcctgtactg
      901 gtcgttcgct aacagataaa agtatttatg ccgccctgcc tggaacatcc cggcagggct
      961 ttttaatgcc ataaaaaagc cgccaggttt gaccccggcg gctaagcaat tgcaggtgaa
     1021 tcttacttct tcttatgcag ttttggtgcg aatcaggtag tcaaaagcac tcagagaggc
     1081 tttggcacct tcgccagtgg cgatgatgat ctgcttgtac ggaaccgtcg tacagtcacc
     1141 cgctgcgaac acgcctttga cgttggtttc acatttcgca tcaatgataa tctcgcccat
     1201 gcggttacgt tcgactgcgc cttccagcca gttggtgttc ggcagcagac caatctggac
     1261 aaaaataccg gccagttcga tgttgtgaat attgccgctg acacgatctc gatattccag
     1321 accaacgact ttgctgccgt cgcctttcac ttccgtggtt tgcgcattca gaataatgtc
     1381 gacgtttttc aggctgcgca gtttgtcctg cagaacctgg tcggctttca tttctggcgc
     1441 aaattccagc agcgttacgt gctcaacgat acccgccagg tcgattgccg cttccacgcc
     1501 ggagttaccg ccgccgataa ccgctacgcg tttgccttta aacagcggtc cgtcgcagtg
     1561 cgggcagtag gtcacgcctt tagtgcgata ctgatcttcg cccggaacgt tcatgttgcg
     1621 ccattttgca cccgtcgcca cgataatgct gcgtgctttc agtaccgcgc cagaagctgt
     1681 ttcaatctgg tgtagaccac cttcaaccgc tgccgggatc agtttgctgg cgctctggct
     1741 gtcgatcaca tcaacgtcgt attcatcaac gtgaactttc agtgcgcctg ccaacttctg
     1801 cccttcagtc ttcggtacag aaatgtagtt ttcgatatca acggtatcga ggatctgacc
     1861 accaaaacgt tcgcccatca gaccggtacg gatgccttta cgtgcggagt aaattgctgc
     1921 cgctgcaccc gccgggccgg aaccaacgat taatacgtca taagcatcac gcttgttcag
     1981 ctcttctgcc gcacgttttt ccgcgccagt atcaattttg gcaacgattt cagtcaacgt
     2041 catgcggccc tgaccaaact ctttcccgtt tacgaacact gccggaacgc ccatcacgtt
     2101 gcgatcggtg atttcgttct ggaaggtgcc gccgtcaatt gcagtgtgct tgatgcgcgg
     2161 gttcaatacg ctcatcaggt tcagcgcctg caccacgtcc gggcagttat ggcaagagag
     2221 cgagtaatag gtttcgaatt caaaatcacc gtcaatatgg cgaatctgct ccagcagaga
     2281 ctgcgcttct ttcgacggat gaccaccggt ccacagcaac gccagtacca gcgaggtgaa
     2341 ctcgtggccc agcggggagc ctgcaaaacg tggtccctgg ttggaacctg ggttggtgat
     2401 caggaaagac ggcttacgca ctggcaagct gttatcttct ttaaaggtga ctttgtctga
     2461 cagttctgcg atttcagcca acagttcctt gatttctgcc gatttagcgc tgtcatccag
     2521 cgtggcaatt aactcaacag gcttggtcaa tttctcaagg taagccttga gttgagtttt
     2581 catatttgtg tcgagcatgt ttatctcctg ggctttaaac atcatcatgc aagcggcctc
     2641 tgcggctacc tggatgcagc ttgcatcatg gtgcggggaa gaaaatcggg tgccgcagca
     2701 cccgaagaat taggtgaatt tccgaagtat ttcgcgtcag agcaaggcga ctagcctgtg
     2761 aatccccggg agcttacaca agtaagtgac cggggtgagc gggcgacgcc aacgccgcta
     2821 tggcgtgaaa gacgaaggaa atttagattt taccaaccag gtccagagac ggagccagag
     2881 ttgcttcacc ttctttccat ttagccgggc aaacttcacc tgggtgagaa gctacgtact
     2941 gagctgcttt gattttacgc agcagatcag acgcgtcacg gccaatgcct tcagcggtaa
     3001 cttcgattgc ctggatgata ccctgcgggt caacaacgaa ggtcgcacgg tcagccagac
     3061 cttcatcttc acgcatgttg tcgaagttac gggtcagggc gccagtcggg tcgccgatca
     3121 tcgcatattt gattttagcg atggtttcag agctgctgtg ccatgctttg tgggtgaagt
     3181 gagtatcggt agatactgcg tatacgtcta cgcccagttt ctgcagttct tcgtagtggt
     3241 cagcaacgtc acccagttcg gtcgggcata cgaaagtaaa gtcagccggg tagaagaaga
     3301 agacgctcca gcggccttcg gtatcttttt cggtgatttc gatgaattcg ccgtttttga
     3361 atgcctggtt tttaaaaggt ttaattttgg tgttaatcaa ggacatctat acttcctccg
     3421 tgttttcgat gagatgtaag gtaaccaatt tttgccgatt cggctaatgc gtttccatta
     3481 tcaaatcgat aagttttacc ttacaacctt cgtaagacaa ctttgtgaac tttgcaccgc
     3541 cagcggcgat aaaaagtaaa atggccccct ttccaggtga ccgtgtaatc tgaactaccc
     3601 tttggtatct tcagcgcctg cctgcaggct gaggtgttgc ccttacaaat gcaacaacga
     3661 catggattac aacaccctca taaacaaagg gcaatcacct gatctaagct cttacctatg
     3721 acagtgatag gttatgcctt ttactcgact tttgcactga ctgaaaagaa caaattaatg
     3781 ttaaaaaaga tacttttact ggctctgctt cctgcaatcg ccttcgcaga ggaacttcct
     3841 tccccagtaa aagcgattga aaaacagggc attacaatca tcaaaacgtt cgatgccccc
     3901 ggaggaatga aaggttatct cggaaagtat caggatatgg gcgtcaccat ctacctgact
     3961 cccgatggta agcacgctat ctctggttac atgtacaacg agaaaggtga aaacctgagt
     4021 aacacactta tcgaaaaaga aatttacgca ccagccggac gcgaaatgtg gcaacggatg
     4081 gaacaatccc actggctcct cgacggtaaa aaagatgcgc cggtcattgt ctacgtcttc
     4141 tccgatccgt tctgcccata ttgtaaacag ttctggcagc aggcgcgccc gtgggtagat
     4201 tctggcaaag tgcaattaag aacattgttg gttggggtta tcaagccaga aagcccggcg
     4261 acagcagcgg caattcttgc ctccaaagat cccgcaaaaa cctggcaaca atatgaagcc
     4321 tctggttgca agcttaagct aaacgtgcct gcaaacgtaa gtacagagca aatgaaagtg
     4381 ttaagtgaca atgagaaact gatggacgat ctgggggcaa atgtcacgcc ggctatctat
     4441 tacatgagta aggaaaatac gctgcaacag gccgtggggt tgcccgatca gaaaacgctt
     4501 aatatcatta tgggaaataa ataaaaggta attcatggat ggttgaacta tatccctgct
     4561 tttcaaccat cctaaaaaag catgaatttc tcgctctacc ctaagtggct gtgctatttt
     4621 agaaaaactt aatcctgagt taagcaaaaa aacataatca ataaaaatac atacgcaaaa
     4681 aacattgact aagtgaatat accatggaag aaaaatataa accggagtag tgtatggcca
     4741 atctctacga cttgaaaaag ttcgacttaa atcttcttgt catatttgag tgtatttacc
     4801 agcatttaag cattagcaaa gctgctgaat cactatatat caccccatca gccgtgagcc
     4861 agtcgttaca acgcttacgt gcgcaattta atgacccttt attcattcgc tcaggaaaag
     4921 gtatcgcgcc gacgacaaca gggttgaatc tgcatcacca tcttgagaaa aacctcaaag
     4981 gccttgaaca aacaattaat atagtcaata agtccgaact aaagaaaaac tttattatct
     5041 acggccctca gctcatttct tgctctaata atagtatgct gatccgctgc ctacggcaag
     5101 atgcctctgt cgagattgag tgccatgata tcctcatgtc agcagagaat gctgaagaac
     5161 tgttggttca ccgcaaagcc gatctggtga tcacccaaat gccagtgatc agccgttccg
     5221 tcatttgcat gccattgcat acaattcgca atactctaat ttgtagtaat aagcatccgc
     5281 gaattactga caatagcact tatgaacaaa ttatggcaga agagttcacc caattaatag
     5341 ccaaatcagc aggagttgat gatattcaaa tggaaataga cgagaagttt atgaatcgca
     5401 aaatttcatt tcgtggttcc tctttgctga caataattaa tagcattgca gttacagatt
     5461 tactgggaat agtcccctat gaactttata actaccaccg tgactttctt aatttaaaag
     5521 agataaaact agaacatccg ttaccttcaa taaagcttta catttcctat aataaatctt
     5581 ctttaaataa tttggtgttc tcccgattta ttgaccgtct aaatgagagt ttttgatata
     5641 taactataga cagctactat aactttcatc tatttattca cagcgcgaat gaaaaataaa
     5701 acactctgct ttacagtgtt tactttataa ttaacagtct tatctcactg ttcaccctgt
     5761 aacgttcaga gagcgtcttc agatgtctat ttataaaatt cctcttccgc tcaatattct
     5821 ggaagctgca cgagagcgta ttacctagac actcaacacc ctaccccgcg tttgtgtctc
     5881 attttctggc ggcaaagact ctggtttaat gctgcatctg acagccgaac ttgcccgaca
     5941 aatgggcaaa aaaatctgcg ttttgtttat caactgggag gcgcaatgaa tcgccacgga
     6001 taatctagac acttccgagc cgttgataat actggttttc atattctgtc ggtgacatct
     6061 gttcgctaga accatgccga cgcttactgt tataaaacat ttcgatgtaa tcaaaaatat
     6121 cactgcgggc ttcttcccgc gttccgtaga tctttttctt tatccgttca cgtttcaaca
     6181 actggaaaaa actttctgca accgcattat catggcagtt accgcgacgg ctcatgctac
     6241 cctccaggcc gt
//
LOCUS       DAJTQY010000161         6222 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.161, whole genome shotgun sequence.
ACCESSION   DAJTQY010000161 DAJTQY010000000
VERSION     DAJTQY010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6222
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.161"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(85..900)
                     /gene="sul2"
                     /locus_tag="OO349_003740"
     CDS             complement(85..900)
                     /gene="sul2"
                     /locus_tag="OO349_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCR5868653.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            2089..2238
                     /locus_tag="OO349_003741"
     CDS             2089..2238
                     /locus_tag="OO349_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5868654.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPV
                     GREAL"
     gene            complement(3005..3445)
                     /locus_tag="OO349_003742"
     CDS             complement(3005..3445)
                     /locus_tag="OO349_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HCR5868655.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
     gene            3663..4052
                     /locus_tag="OO349_003743"
     CDS             3663..4052
                     /locus_tag="OO349_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5868656.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            4096..4500
                     /locus_tag="OO349_003744"
                     /pseudo
     CDS             4096..4500
                     /locus_tag="OO349_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(4607..5443)
                     /locus_tag="OO349_003745"
     CDS             complement(4607..5443)
                     /locus_tag="OO349_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HCR5868657.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(5443..>6222)
                     /locus_tag="OO349_003746"
                     /note="aph(3'')-Ib"
     CDS             complement(5443..>6222)
                     /locus_tag="OO349_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HCR5868658.1"
                     /translation="GESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASRRGELAGER
                     DRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAWPSMGQQLG
                     AVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLLARVERELP
                     VRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLALMIANAEE
                     NWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
BASE COUNT         1438 a         1624 c         1642 g         1518 t
ORIGIN      
        1 aaaaaaaata ttagttcgat tcaatggagg ttccttcagt tttctgatga agcgcgaata
       61 tagagaaata tcccgaatgt gcagttaacg aattcttgcg gtttctttca gcgccgccaa
      121 taccgccagc ccgtcgcgca aggggcgcgg ctcgtgtgtg cggatgaagt cagctccacc
      181 tgcggcggcg gcaagctctg cagcgagtgt cgcggccccg acatcccccg gaccacggcc
      241 tgtgagcgcg cgcagaaagg atttgcgcga aacagacaga agcaccggca aatcgaagcg
      301 cagccgcaat tcatcgaacc gcgccagcac cgagagcgag gtttcgggag cagcccccag
      361 aaaaaacccc atgccgggat caaggacaag gcggttgcgt ttgataccgg cacccgtcag
      421 cgccgcgatg cgcgcgtcaa agaacgccgc aatgtgatcc atgatgtcgc cagcgggtgc
      481 ctcgcgccga tctgcctgcc cgtcttgcac cgaatgcata acgacgagtt tggcagatga
      541 tttcgccaat tgcggataga acgcagcgtc tggaaaaccg cgaatatcat tgagataggc
      601 cacaccacgc gacaaggcat aggcttgcgt cgcgggttga taactgtcga gcgagacggg
      661 aatgccatct gccttgagcg cgtccagcac cggcgcgata cgcgcgattt ctgtgtcgga
      721 cgaaacaggc gcggcgtcgg gattgctgga tgccggaccg aggtcgatca catctgcccc
      781 ctcggccatc agcttacgcg cctgcgcaat ggctgcgtct ggcgccagat accggcctcc
      841 atcggagaaa ctgtccgagg ttatgttgac gatgccgaaa atgatgagcg atttattcat
      901 gggggcttct ataataataa taatcgagca tgagtctcat acggatgctc gggtcgaaag
      961 ggaatcccca ggcgagtaac ctgtttgcgg tgatccgttt catgatgatg tatctgtagc
     1021 attttggaaa taaatatatc atctctcaat aagtagatat atttatcttt tttgcttctt
     1081 tggtgctgca agtcaccaat acgagggaaa cctgttacgg agcggtttaa ccgtaatcaa
     1141 cagtgactgg tgtcatgttc ggaagattaa ttgatagtaa tctacaaagg atcttcttga
     1201 gatccttttc aatgaatcgt aatctcttgc tctgtaaacg aacaaaccgc ctggggaggc
     1261 ggtttgttcg aaggttaagt cagttgggga actgcttaac ccggtaacag ggttcacgag
     1321 accgccatta ccaaaactgt tctttcagtg tagccgcagt caggcctcta cttcaagacc
     1381 tcttaatcta tcttctcgtg cactcctgtt actaatggct gctgccagtg gcgttttgtc
     1441 gtgtctttcc gggttggact caagttgata gttaccggac aaggcgcagc ggtcggactg
     1501 aacggggggt tcgtgcatac agtccagctt ggagcgaact gccttcacgg aactgagtgt
     1561 caggcgtgga atgagaaacc gcggccataa cagcggaatg acaccggtaa accgaaaggc
     1621 aggaacagga gagcgcacga gggagctacc agggggaaac gcctgggatc tttatagtcc
     1681 tgtcgggttt cgccaccact gatttgagcg tcagattttg cgatgcttgt caggggggcg
     1741 gagcctatgg aaaaacggct tcgccgtggc cttatctctt ctttgctaag tgtcttcctg
     1801 gtatcttcca ggaaatttcc gtccccgtcc gtaagccggt accgctcgcc gcagccgaac
     1861 gaccgagcgt agcgagtcag tgagcgagga agcggaatat ctcctgtatc gcatattctg
     1921 ctgacgcgcc ggtgctgcct tttttctcct gccacatgaa gcacttcact gattttcgca
     1981 atcgtggcaa catagtaagc cagtatacac tccgctagcg ctacatgact ggttcagggc
     2041 tgcgccccga aacccgctaa caacctctca gctactggat atcaccccat gccatctgaa
     2101 tatgatgaac aactggattc gactgatacg cagcttcccg gcgaatatga cctttttgat
     2161 ttaatggcgc aggttactga agagaacctg catgcccctg tggattttgg gtcgcctgtc
     2221 ggccgggaag cgttatagct ttttcgcttg cccgcgtacg tggtaatact ccggtattac
     2281 cttttatttc tttattttca tgtaattaca gtggattttt aatacccggg tattactcgt
     2341 gaaaacgaaa tattcctttt ttttctttta attcatgcag ttgcgatgaa aatcgcggct
     2401 gcgttaggtg tatgaccatt ttgaggttga aaaataatca accatggcgg gtgatttacg
     2461 caggttaagg ggagatcgtg acaaaaaaag cgttcctcac tgtatcgttc atgcctgcat
     2521 gaacgggtta ttcttcctga ctacagaatt acatcgctcc ttcagttcat acatcacttt
     2581 tacatcactt atacatcact tttacatcac ttatacatca cttttacatc attccttcag
     2641 ctcatacgtc actttcatcc tctgtgccat ttgtacatca cccccttcct gaacgtgctg
     2701 acagcgggtt ttccggctgc gccggcaacg cttcacggcc acgcaataaa ttgcgtcccc
     2761 gttcaccgtt ttcggatggg aagaacgccg gaaacaggac ttaccccggc tgaatggtgt
     2821 gaatttccgc gctatgcact tgtgcgcata ctcatgcatg ccgtaaaaac agagcctgcg
     2881 cgtttctggc gggttttcgg gtggtttgtt gcctgtttta ccggtttccc gtcagaaacg
     2941 ccctgagggc ctctcaggcg gtgctgataa cagatgttat ggtaaatcag agtcacctgc
     3001 actgttactc tgaaccggag cgaaacagcc gcgaccagaa tccccgcttt ttttcctgtg
     3061 ccagcgcgtc agccagacgg ctaatttcgg cctgtagttg ttccctctcc tcatttcgca
     3121 ggcggttttc tgcttcccgc gccatcttgt cttcaagcat tagggtaaca gtctgctgca
     3181 gagcgtccag tttctgctga atgacgtcta gctgagcatt ttccgcctgg ctaacgacag
     3241 atttggagtg tccctccttg tgcctttttg gtgtctcaga agtgcgcagc tcgccatagg
     3301 cccgaatcag ttcactggtc tcgaactccc ggcgatcatt ttttcctgtg cgatagctga
     3361 caaggccttt agccatgtca cgatataatt gcgagcgcga ctttcctgtt aatttcagtg
     3421 tctcttgaac tgtgtgccat gccataaaag tgacagtgtc tcctgtgtgt accgtattgt
     3481 aacagcagaa catattactg ttttaacaca aaaggtctga ggctgcccgt gaggcgtgaa
     3541 aaatacacaa aaaacctgtt gacggtgtgg ttttgacggg cgtagtattt cgaatactgt
     3601 atataaacac attgttataa atacagtttt tgctgttttt aaaagtcaaa gaggataatt
     3661 ttatgagtac agagttaaat agcggtactg tcggtggttt tggtcacatg ggaccaaatg
     3721 acccctacaa tgatggtcat agcggctctg gaagttcggg aggtagtttt aacggtggtg
     3781 gtaatggtgg ccatgataat agtagtggtt catcttatac tgcatccttt ggtctcatca
     3841 ctggccgaca cccgataact gtcccaggta cgaagaatat taattctgtc gatctttctg
     3901 ctgtcattaa taacattata catcatgaag gtgcacgcaa taacatgtac ctagatactg
     3961 cgaacaatgt aagagtggga gtgacgggca ctggctggca atgtctagca acggcaggca
     4021 tttcggctga gggtaaaaga actttccgct aagcgataga ctgtatgtaa acacagtatt
     4081 gcaaggacgc ggaacatgcc tcatgtggcg gccaggacgg ccagccggga tcgggatact
     4141 ggtcgttacc agagccaccg acccgagcaa acccttctct atcagatcgt tgacgagtat
     4201 tacccggcat tcgctgcgct tatggcagag cagggaaagg aattgccggg ctatgtgcaa
     4261 cgggaatttg aagaatttct ccaatgcggg cggctggagc atggctttct acgggttcgc
     4321 tgcgagtctt gccacgccga gcacctggtc gctttcagct gtaagcgtcg cggtttctgc
     4381 ccgagctgtg gggcgcggcg gatggccgaa agtgccgcct tgctggttga tgaagtactg
     4441 cctgaacaac ccatgcgtca gtgggtgttg agcttcccgt ttcagctgcg tttcctgttt
     4501 ggggtcgttt gcgggaaggg gcggaatcct acgctaaggc tttggccagc gatattctcc
     4561 ggtgagattg atgtgttccc aggggatagg agaagtcgct tgatatctag tatgacgtct
     4621 gtcgcacctg cttgatcgcg gccgcgatag ctagatcgcg ttgctcctct tctccatccg
     4681 cgttccaagc tgcggaaagg cacccataag cgtacgcctg gtcgagcagg cgacgcggat
     4741 cgacgtccag cgcacgagag aatgcgtccg ccatctgtgc aatgcgtcta ggatcgagac
     4801 aaaggtcgtc tctgtcagcc ggatcgtaga acatattggc ggcgccaaag cccacttcac
     4861 cgaccagacc gacgggatct atcaccagcc agccgcgact ggagaacatg atgttttcat
     4921 gatgcagatc gccatgtagc ccacgcagtt ccgaggcatt gctcatcatt tgatcggcta
     4981 taatcgccgc gtggacgtag tcagtttgac aacctgcgtt ttgatcatcg cgcgcccgct
     5041 gaaacaaagc tgcaaagcga tcccggatcg ggagaagggc agaaggcagg ggttcctcag
     5101 atgcggcata cagcttcgcc attagttccg ctgcaatttc ggtcgcctgg tagtcgccgt
     5161 gctcggcaac gatgtgagag agcattcgct ccccggcata ttcgagcaac atcagattgt
     5221 tctcacgacc gagcaaccgg actgctcccc tcccattgcg ccataccaga tagtcggccc
     5281 cgcgcagttc atcagcaatg tcttctatag gtttcaatcc cttgacgatt gcaggagtcc
     5341 cgtctggcaa tgaaactttc caaacgaggc tggaaaaggt gtccgcaatg agaacaggtt
     5401 gcgaaacgtg ccaatgagca ggaaaaacag gcggcatgaa catcaacccc aagtcagagg
     5461 gtccaatcgc agatagaagg caaggcgttc gcggtcgggg gcttcgatcc ccaatacatt
     5521 gaataggaca gcgaaggcgc gctctgcttc atctggcgct gcccagttct cttcggcgtt
     5581 agcaatcatg agtgccaaat cggcatagcg atctgctgtt ccgagccgcc caaggtcgat
     5641 cagacccgtg cattgaagag ttttagggtc caccatgaag ttcggcatgc agggatcacc
     5701 atggcaaaca accatatcgg tgcgctcttg gtcgagccgc accggtagct ctcgttcgac
     5761 acgagccaaa agatcgagct gcggcgtact cttgtcctcg tccggtaaga agtcgggatt
     5821 gacggcattg cgggacacca catcaacggc gcgtccgaac attcgcgaca gcctgcgctc
     5881 aaacggacat tgatcaaccg ataggctgtg aacagcgcca agttgctgcc ccattgacgg
     5941 ccacgctttg agcaaatccg ctccagacag atcagccgcc ggtactcccg gaattgccgt
     6001 tatcaccaag catgcaccct cctgttcctc ctgccagttg atgacctcgg ggcaagccac
     6061 acctcgacct ttgagccaaa tgaggcggtc acgctctcca gcgagctcac cgcggcggga
     6121 agcaggtgcg attttcgcga aggcatgccc gtcaccacgt cgaaaaacaa aatcaccaga
     6181 ttctccgcct ctgacaggca accagtcaga atgcgattca cc
//
LOCUS       DAJTQY010000162         6211 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.162, whole genome shotgun sequence.
ACCESSION   DAJTQY010000162 DAJTQY010000000
VERSION     DAJTQY010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6211
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.162"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(228..431)
                     /locus_tag="OO349_003747"
     CDS             complement(228..431)
                     /locus_tag="OO349_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5868659.1"
                     /translation="MNETELKHVIALLLEDAKRLQQLEPNAGTGARIWLAKEALESGD
                     YDSEEAFYKAEGRAGYSPGLGGV"
     gene            complement(433..588)
                     /locus_tag="OO349_003748"
     CDS             complement(433..588)
                     /locus_tag="OO349_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HCR5868660.1"
                     /translation="MNTIDLGNNESPVYGVFPNQDGTFTAITYTKSKTFKTEAGARRW
                     LGRHSGE"
     gene            complement(791..1198)
                     /locus_tag="OO349_003749"
     CDS             complement(791..1198)
                     /locus_tag="OO349_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5868661.1"
                     /translation="MKNETFGARLLHRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPAILTEDQKELLQLFDALPES
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
     gene            1275..1502
                     /locus_tag="OO349_003750"
     CDS             1275..1502
                     /locus_tag="OO349_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR5868662.1"
                     /translation="MLKIDAIAFFGSKTKLANAAGVRLASIAAWGELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKAKRPGKVIHENQA"
     gene            1486..1908
                     /locus_tag="OO349_003751"
     CDS             1486..1908
                     /locus_tag="OO349_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5868663.1"
                     /translation="MKIRHEHIESVLLALAAEKGQAWVANAITEEYLRQGGGELSLVP
                     GKDWNNQQNIYHRWLKGETKAQREKIQKLIPAVLAILPRELRHRLCIFDTLERRALLA
                     AQEALSTAIDAHDDAVQAVYRKAHFSGGGSPGDSVVVH"
     gene            1986..2769
                     /locus_tag="OO349_003752"
                     /pseudo
     CDS             1986..2769
                     /locus_tag="OO349_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309971.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2776..3522
                     /locus_tag="OO349_003753"
                     /pseudo
     CDS             2776..3522
                     /locus_tag="OO349_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            3537..3959
                     /locus_tag="OO349_003754"
     CDS             3537..3959
                     /locus_tag="OO349_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HCR5868664.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGRVTIRQLSDETGISRASVG
                     RLCIELVASGDVYNSGYGLFPSEQARKDWQSARKKLSRVKVRKPVVVDPDLIWSLPDG
                     EIRRYDRRLNIICRECRKSEAMQRVLAFYQGNFQEAIL"
     gene            4017..4373
                     /locus_tag="OO349_003755"
     CDS             4017..4373
                     /locus_tag="OO349_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5868665.1"
                     /translation="MEIKPEDELSNIVLFPIKEDDPRNQVNFLYKPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRRNIEKLIQIE
                     RNAKARIRRATKSRTE"
     gene            4542..5207
                     /locus_tag="OO349_003756"
     CDS             4542..5207
                     /locus_tag="OO349_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HCR5868666.1"
                     /translation="MTVADFKRPKLELPNGANKLLLHSCCAPCSGEVMEALQASGIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNDCGRRAVAHYPGMVYWD
                     YNWRKQGGSSRMIEISKREKFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKE
                     EKE"
     gene            5435..5590
                     /gene="hokD"
                     /locus_tag="OO349_003757"
     CDS             5435..5590
                     /gene="hokD"
                     /locus_tag="OO349_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HCR5868667.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYESRK"
     gene            5840..6127
                     /locus_tag="OO349_003758"
     CDS             5840..6127
                     /locus_tag="OO349_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5868668.1"
                     /translation="MKIEELREIFSENGLYAVRVENGAIVSHCRIRCLQSQQRKSGAV
                     LFYFCNGLLTDGFILREDEFVTSLRVLKKIGFKAGFLLLLKNKLIYNLEQD"
BASE COUNT         1641 a         1350 c         1661 g         1559 t
ORIGIN      
        1 agccagagga ttacgaaggg cttcataaat atatgctctc actaggattc aggagaacga
       61 tcccccatgg agatggttcg tataatcaac tcccggacgg aacttatgtt tccgaaaagg
      121 gcggtgatat ttatcaaatt cgcagccaga tatctgacta tgcagaccga ctatccgggt
      181 atcgcgcgtc tgtttttgtt tgcgaattca gtcaatgcgc atggtattta taccccgcca
      241 agacccggtg aatatcctgc acggccttct gctttgtaga aggcttcttc gctatcataa
      301 tcgccagatt ccagcgcttc tttagccagc cagatgcgtg ccccagtgcc tgcatttggc
      361 tccagttgct ggaggcgttt tgcatcttct aggagtagag cgataacgtg ttttaattct
      421 gtctcgttca ttttactcac ctgaatgtct tcccaaccaa cgacgtgcgc cagcttcggt
      481 tttaaacgtt ttgcttttgg tatacgttat ggcggtgaat gtgccgtcct ggttggggaa
      541 cacgccgtac accggagatt cgttgttgcc aagatcgata gtattcatgt tgaccccatt
      601 tccccttaac gccggggtag cggaactgtt tgctgagaac accgtgcggt gtcttgatga
      661 ataaaattta gaataaccta aggagggtga tcaagatttt tatgtagaaa aacctaagtt
      721 ttttgatgta aaaaacacaa gtgtttgaaa gtttgtgctt tttattacag ggtgtggaga
      781 aaaaagggga ttatttgttt gcgcttcttt tgcgagcttt gagtagttct tcaaaaagtt
      841 tgttgaaatt ttcaactcga gcacgcatct ctgacaacag ggccttttgc tctgactcag
      901 gcagtgcgtc gaacagttga agtaactctt tttgatcttc tgtcagaatg gctggctgat
      961 tatccgggat cggttcgcct ggttgtttat cttcatcccc aaaaagaagc caagtcggtg
     1021 agcactgaag cgcttggctc agtgcgaata atcttttccc cgccggctgt gtttcatctc
     1081 tttcccattg agaaattgtt acgtgagcca ctttgaccag cttacctaat gcggcctgag
     1141 acagttttaa ttttttacgc ctatgtaaga ggcgagcacc gaaggtttcg tttttcatat
     1201 tagggaattc taatttttct tgacttaggt ttctctacgg cctggtttcc ttaggaaaat
     1261 ctaaggagct cgatatgttg aaaattgatg ctatagcgtt ttttggcagc aaaacaaagc
     1321 ttgccaatgc cgcaggagtt aggttggcaa gcattgctgc atggggggaa ctggttcctg
     1381 aaggtcgcgc gatgcgcctg caagaggcat ctggcgggga acttcagtac gaccccaaag
     1441 tttatgacga atatcgtaag gcaaaacgac ctgggaaggt gattcatgaa aatcaggcat
     1501 gagcacatcg aatcagtgct gttagccttg gcagccgaaa aagggcaggc gtgggtcgct
     1561 aacgcaatta ctgaagaata tctgcggcag gggggcggcg aattgtctct ggtaccaggc
     1621 aaggactgga acaatcagca gaatatctat caccgttggt tgaaaggtga aacgaaagcg
     1681 caaagggaaa aaattcagaa actgatccct gcggttctgg caattcttcc gcgtgagctg
     1741 cgtcaccgac tctgtatctt cgataccctg gaacgccgtg cattactggc ggcgcaggaa
     1801 gcgttgagta cggcaattga tgcgcatgat gatgctgtcc aggccgttta ccggaaagca
     1861 catttcagcg gtggtgggtc gcccggcgat tctgtcgtag tgcattgatt gaaattaatc
     1921 gtgccggatt gttttgttcg gtatcagtta aatgtaacgc tgcgagcgtt acaaggtgaa
     1981 aacaaatggc ttcaaactgg ataaagctcg aggttattac gccggataag ccggaaatat
     2041 tcaggcttgc tgagattctg aatattgatc cagatgccgc attagggaag gttattcgct
     2101 tctgggcatg ggcggatcaa caaatgatag acggtaatgc agattgtaac gctcgcggcg
     2161 ttacaaaaag tgcaatagat cgcatcactt ttatgtctgg ttttgctgat gcgttaattc
     2221 aggttggatg gctggtcgaa aatgacggtg ggctttctct acctaacttt gaacgtcata
     2281 acggaaaaag ctctaaaaaa cgggcggtta caaacgagcg agtaacaaaa atacgcgaac
     2341 tgaaacgaaa aggtaacgct gccagcgtta cacaaacgga tcaaaaagcg ttaccagtgg
     2401 aagaggaaga ggaagaggaa gatctaaata ctgatctccc cctaaatccc cctcgccaaa
     2461 aacgagcgtc taaaaaattc gagccggagg ctattgagct gcccgattgg ttgccggaaa
     2521 cactctggca tgagtgggtc cggttcagac aggcattgcg aaaaccgatt cgaacggagc
     2581 agggcgatac gggaactgga aaaattccgt cagcagggtt ttacacctga gcaggtgatt
     2641 cgacacagca tcgccaatga ataccagggc ctgttcgcgc cgaaaggtgt tcggcctgag
     2701 acgttgctcc gacaggttaa caccgtctcg ttgccggaca gtgcgatccc gccaggcttc
     2761 agggggtaac ggaccatgaa aaatattgcg acaggcggcg ttctggaacg catccgccga
     2821 ctgaccccgc aacatgtaac cgccccattc agaacggtag cggagtggcg cgagtggcaa
     2881 cttgctgaag gccagaaacg tagcgaggag atcaaccgcc tgaatcgcca gttgcgggtg
     2941 gaaaaaattc tgaatcgctc aggcatccag ccgttgcacc gtaaatgctc gtttgcgaat
     3001 taccaggtgc agaacgacgg ccagcgatac gcgttaagcc aggcgaaatc catcgccgat
     3061 gaactgatga ccgggtgtac aaattttgcg ttcagcggaa aacctgatac cgggtagaat
     3121 cacttagcgg cagctatcgg gaatcgcctg ctgaaagacg gtcagacagt gattgtggtt
     3181 accgtggctg atgttatgag cgccctgcac gccagctatg acgatgggca gtcaggcgaa
     3241 aaatttttgc gggagctgtg cgaagtggat ctgctggttc ttgatgaaat tggcattcag
     3301 cgcgagacga aaaacgagca ggtggtgctg caccagattg ttgatcgccg gacagcgtcg
     3361 atgcgcagcg tggggatgct gacaaacctg aactatgagg tgatgaaaac attgctcggc
     3421 gagcgggtga tggatcgcat ggtcatgaac ggcgggcgct gggttaattt taactgggag
     3481 agctggcgtc cgaatgttag ccattcgagg gttgttaagt agtttcagga ggatttatgg
     3541 cgaaaccttt tactcccgaa cagcgggaag aactgaagac gcgaattgtg gaactcgtgc
     3601 atcaggacgg tcgggtcacg attcggcagt tgtccgatga aacaggtatc agtcgtgcgt
     3661 ctgtcggtcg cttatgcata gaactggtcg caagtggtga tgtatataat tctggctacg
     3721 gcttattccc gtctgaacag gctcgcaagg actggcaaag cgcccgcaaa aaactctcga
     3781 gagtaaaggt gaggaaaccg gttgttgttg atccggacct tatctggtca ttacctgacg
     3841 gagaaatacg ccgctacgac aggcgcctga atataatctg tcgcgagtgc cggaagagcg
     3901 aagctatgca gcgtgtactg gctttctatc agggtaattt tcaggaggcg atactgtgaa
     3961 tgaaattagc tatcaggctt caattaccgc tggcattcgc atcaaaggag aggagcatgg
     4021 aaataaaacc agaagatgag ttaagcaata tcgttttatt tccgataaaa gaggatgacc
     4081 ctcgtaatca ggttaatttt ctttataagc catcggaaag accatattgc catcacgcct
     4141 ctgtacgggt tgacgaaaaa gagcgtcagg tccgctgtaa aatctgcggt gcggttgtgg
     4201 agccgtttga ctggatgctc tctgtggcaa aaagagaaac cagactggca gatgatgtaa
     4261 ggctattgcg ccaggaggaa caggaaaggc ggagaaatat agaaaagctg atacagattg
     4321 agcgtaacgc gaaagcgcgg atacgcaggg cgacaaaatc cagaactgaa taattaaatt
     4381 tagcactgtt aaaaatttaa tccttaaccg gagggatttc tgcaccctca aatcatcagg
     4441 agaccgcccg aaagggcggg gagcagtcac acatctgttt ccgatagccc cgttctaatg
     4501 ctacactctt tgatattttt atgacctcaa taaacatatt tatgacagtt gctgatttca
     4561 aacggcccaa attggagctc ccaaacgggg caaacaaact actactgcac tcttgctgtg
     4621 ctccatgttc cggtgaggtg atggaggcgc ttcaggcctc gggaatcgac tacaccatct
     4681 ttttctacaa cccgaacatt catcctcaga aagagtattt aattcgtaag gatgagaata
     4741 ttcgctttgc tgaacaacac ggcgtgccgt ttattgatgc tgattacgac acagacaact
     4801 ggtttgaacg agccaaagga atggaatggg agcccgaacg agggatccgt tgcaccatgt
     4861 gttttgacat gcgttttgag cggacagcgc tgtacgccgc tgaaaatggt ttcagtgtga
     4921 tcagcagttc actgggcatt tcacgctgga aaaatatgca gcaggttaac gactgtgggc
     4981 ggcgagccgt cgcgcattat ccgggcatgg tgtactggga ttataactgg cgcaagcagg
     5041 gcggctcgtc ccgtatgatt gaaatcagca agcgcgaaaa attctatcag caggaatatt
     5101 gtggctgtgt gtattctctg cgcgatacca atctacaccg caaatctcag ggacgccctc
     5161 ttatcaaaat tggtcaactc cactacggta aagaagagaa ggagtgattt tatggggcac
     5221 ctttctgatt gatttcatat tggcgaggta agtagaatga ctgcgggtgc ttgaggctat
     5281 ctgcttcagg catgaacacc aaaaggcaga tagagaaaag ccccagttaa cattacgcgt
     5341 ccggcaagac gcttaacatt aatctgaggc caatttcatg ctttgcacat gtaggttagc
     5401 ctcttacatg ccgaaaggca aggagaagca ggctatgaag cagcaaaagg cgatgttaat
     5461 cgccctgatc gtcatctgtt taaccgtcat agtgacggca ctggtaacga ggaaagacct
     5521 ctgtgaggta cgaatccgaa ccggccagac ggaggtcgct gtcttcgtag actacgaatc
     5581 caggaagtaa gagtgaccgg gtggggagct gatcccatcc ccgcccacct ctgatgtgtc
     5641 aggcatcctc aacgcacccg cacttaaccc gcccatcgct gtgatctctc agcgtttcgg
     5701 cgggtttttt gttgtttatt tccggtgaat ttgattcgcg caccttcgca gatagaatcg
     5761 actcacttaa gtagcgcgca gggagaagag ggatggaccc cgaacagggg agagctattt
     5821 atctggaagg attctgaaga tgaaaatcga agaattgcgt gaaattttta gtgaaaatgg
     5881 cctctatgct gtgcgcgttg agaatggagc gattgtcagc cattgccgca ttagatgttt
     5941 gcaatctcaa caaaggaaga gcggtgctgt gttattttat ttttgtaatg gacttctgac
     6001 ggacggtttt attttgcgtg aggacgaatt tgtcacatca ttacgggttt tgaaaaagat
     6061 tggttttaag gctggttttc tgcttttgct gaagaataaa ctcatctaca atcttgagca
     6121 ggattgaact cctgctgtgt aacaccgtgc caccggagaa agccgatggc acatatacaa
     6181 ctggtcaaac aaacctcttc cggattactt c
//
LOCUS       DAJTQY010000163         6065 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.163, whole genome shotgun sequence.
ACCESSION   DAJTQY010000163 DAJTQY010000000
VERSION     DAJTQY010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6065
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.163"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(<1..1448)
                     /locus_tag="OO349_003759"
     CDS             complement(<1..1448)
                     /locus_tag="OO349_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCR5868669.1"
                     /translation="MKAIDNIFKYIPSNHKDKHSDKVNNHQHHSKVDKTHRSKIVEID
                     KLDNDSQIDNDFGMHIIYFLQHGHWRVNEHSLQMEKIWFYNSEPSIDIQEYNSFADNT
                     TNTFIFTIIPDNNHVIKLSSPITVKIECKDGYYFINFSGDKSDIIYKVNGLSIIPENF
                     FTLLSGNFKADWRWDVSKETFPKDKFDSYVKSVFSKIDFYKQCGVINPQNANTAYFGD
                     TDGRVGAVLYSLLVSGHIGIREKGWSLLCELLKHEDMASSAYIHKNNKLLYNLLNTRD
                     MILNELHQHVFLKDDAITPCIFLGDHTGDRFSTIFGDKYILTLLNSMRNMEGNKDSRI
                     NKNVIVLAGNHEINFNGNYTARLENYKLSAGDTYNLIKTLDVCNYNSETKVLTSHHGI
                     IRNEENQCYCLGALQVPFNQMKDPIDPEELANICNKKHKQHMDDHLLHLIRSNAIAPT
                     PVYENYFNNTTAFRPKPEDIFKCGQTLKKTKQ"
     gene            1833..2864
                     /locus_tag="OO349_003760"
                     /pseudo
     CDS             1833..2864
                     /locus_tag="OO349_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 family protein"
     gene            complement(2951..3427)
                     /locus_tag="OO349_003761"
     CDS             complement(2951..3427)
                     /locus_tag="OO349_003761"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5868670.1"
                     /translation="MTNKKMYFILILVFTLLQVCFFALWKARDGSTTSLECTSTLTRN
                     AKTDHSLYYSANLSVILKKDGSGSFTIVGLTDEDTPRKFSHSYFFTYKIDSNGRISGN
                     AKAKVSGLENQIKDENFRLNFLDASLTGKGNARLSKFNNVYIFSIPGLIINTCAPI"
     gene            complement(3420..4184)
                     /locus_tag="OO349_003762"
     CDS             complement(3420..4184)
                     /locus_tag="OO349_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HCR5868671.1"
                     /translation="MERRCFFIINDAIIFDPNKCSLSNVNTQDEINLPATAGCILAVL
                     LESNGGAVDRNSIIDSVLERFSFDLSNNTLNQYISLLRKSFKNIGVEENVILTIPKVG
                     FYISENITISKQSYDDSHLQEDVVEKVERKRSIPLFRILISLVVVIVIIEIFAVAFKD
                     TYSITDYPLVKSGKIDTCDLYLSENLNEIGLAKSQIEAAEISKKHLPCVPGANFIYDV
                     NSMHYLLGEGQRYLSRCIKSSNDETYAICSEVLLYD"
     gene            4430..5191
                     /locus_tag="OO349_003763"
     CDS             4430..5191
                     /locus_tag="OO349_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR5868672.1"
                     /translation="MLSKITQELLKMNNVLLMRPDDSEFMIPDDWSGLNAKGITYEQD
                     NRVTESFLPFKSFYCSPVCTTVELISQNNCFAMGIRYLLEKKRDLRLNHFKAFSHKCE
                     TKPDVLIMTGCTVADTYSIFSRIVEENIMKTMTPIVIITGHHSEDLCSLASKLGGVKI
                     ISPTEKIAKIENAINDSFNSKPKKIRFLTDILSPQQIETMKMVAQGRSVNEISAAMNI
                     SIKTVYAHKVRAINKLGIKKKVYEALFYKALIQFN"
     gene            complement(5298..5798)
                     /locus_tag="OO349_003764"
     CDS             complement(5298..5798)
                     /locus_tag="OO349_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5868673.1"
                     /translation="MLAIMLAGTAFASQAGTLVSQGTEASANLTLTKPIVVNNTIQPV
                     KGVYSGTLTAWTPLATGIVGASDGQSHDYAVTFPDDIYAESSTSADAVISGDNNPDHK
                     LKVSLTTLEQDPPSAASEEIGGKRYMMLKNTGTGGAYRVVSHMKEQVVEPDSYTIRTQ
                     AYIYAE"
BASE COUNT         1875 a         1188 c         1066 g         1936 t
ORIGIN      
        1 ttctgtttag tttttttgag cgtctgtcca catttgaaga tatcttcagg ttttggtctg
       61 aaagcagtag tgttattaaa ataattttca tatacaggtg tgggtgctat agcattgctt
      121 ctaattagat ggagaagatg atcatccatg tgttgtttat gttttttatt acatatattt
      181 gctaattcct caggatcaat tgggtctttc atctgattaa atggaacctg caaagcaccg
      241 agacaataac attgattttc ttcatttctt attattccgt gatgactggt taaaacttta
      301 gtttctgagt tataattaca aacatcgagc gttttaatta agttatacgt atcaccggca
      361 gagagtttat aattttctag tcgtgcagta tagttaccat taaaattgat ttcatgattt
      421 ccagccagaa cgatgacatt tttattaata cggctatctt tattaccctc catatttcgc
      481 atagaattta gcaaagtaag gatatattta tcaccaaaaa tagtactaaa tctatcgcct
      541 gtatgatccc caaggaagat acaaggggtt atcgcatcgt cctttaaaaa aacatgttga
      601 tgcaactcat tcaatatcat gtctctcgta tttaataaat tgtagagcaa tttattattt
      661 ttatgtatgt atgcagatga agccatgtct tcgtgtttta ataattcaca taataaactc
      721 caaccctttt ccctgatgcc tatatgccct gaaacgagca atgaataaag tacagcgcct
      781 actcttccat ctgtatcgcc aaaataagcc gtgttggcat tttgtgggtt aatcacacca
      841 cattgtttat aaaagtctat tttagaaaac acagatttaa catagctatc aaacttatcc
      901 ttaggaaacg tctctttaga gacatcccaa cgccagtcag ctttaaaatt accgctaaga
      961 agagtaaaga aattttcagg tatgatagaa agcccattaa ctttataaat tatgtctgat
     1021 ttatctccgg agaaattaat aaaataataa ccatccttac actcaatctt aactgtaatg
     1081 ggagacgata attttatcac atggttattg tctggtataa ttgtaaagat aaacgtgtta
     1141 gtagtattat cggcaaaact attatattct tgaatatcta tagatggctc actattgtaa
     1201 aaccatattt tctccatctg aagactatgt tcatttaccc tccagtgacc atgttgcagg
     1261 aaatagatga tatgcatgcc aaaatcgtta tctatttgcg aatcattatc cagcttatca
     1321 atttcaacta tctttgaacg atgtgtttta tcaaccttgc tatggtgttg atgattatta
     1381 actttatctg aatgcttatc tttatggttt gaagggatgt acttaaatat attgtcaatt
     1441 gctttcataa ataacccgcg atatttttga cctgttagta aaaaataaat gatacatgtc
     1501 tgaaagacat atttacgacg atataaaaca ataatactta tatgttcgct gattttaagc
     1561 accgagacta tccggtcttt tactaaataa tggaaaacaa tccatccctg gaagacaatt
     1621 gtaaaaaaaa acaacagaat aagaattaat cttaggataa atatttattt atcatggcct
     1681 tttgaccgct aaaaaatggc actcggcaca gtaaaccccg ctatagttat gctatgcatt
     1741 tgtctttaat gatatctcgt cgttatatta acaggctgaa attatcatta atgcacattt
     1801 caatattagc agggatacct gaggagttta aagtgaacat atatatcggg tagcttttca
     1861 aattaatccc tttgattata ggcttaattt gcatcgcgtt aggtggcttt gtgctggaaa
     1921 gttcagggca acgtcacggg tcatgtgctg atttctctgg cggccatatg cctggcatta
     1981 ttcactaccg catttattat catttcgcaa ctcacgcgcg gcgttaatac gttttacaat
     2041 acattgttcc ccattattgg ctatgcgggg tcaattatca ccatgatatg gggttgggca
     2101 ctgttagcag gtaatgatgt gatggcagac gagtttgttg ccggccatgt tatttttggc
     2161 gttggtatga ttgccgcatg tgtatcgacg gtggcagcgt cgtccggtca ctttctgctc
     2221 attcccaaaa atgcagcggg gagcaagagc gacggaacac cggtacaggc ttattcttca
     2281 ttaatcggta attgcctcat tgccgttccc gttttactca ccctgctcgg tttcatttgg
     2341 tctattacgc tgttacatag tgccgacata actccgcatt atgtcgcctt gggttaaccg
     2401 caatctgtgc ctgtctaatt ggcctagtgg ccacaatcgt ccatcaaaca cgtaatacgt
     2461 tctcatccaa agagcactgg ctgtggtgtt attgggttat ttttctcggc tcagtcacgg
     2521 tgctgcaggg gatatacgtc ttagtcagtt ccgatgcaag cgcccgactg gctcccggca
     2581 ttattctcat ttgcctcgga atgatctgtt acagcatatt ctcaaaagtc tggctactgg
     2641 cactggtctg gagacgtacc tgttcgttag ccaacagaat accgatgata ccggtattta
     2701 cctgtctgtt ttgcttgttt ttgtcatcat tcctcgcgga aatcgcacaa actgagctgg
     2761 actactttat tccatctcgg gttctggttg gattaggcgc agtatgtttt acattgttct
     2821 ctattgtttc aatattagag gccggttctg cgaaaaaaaa ttaaaattta cataatccca
     2881 gcgttattca tatcttaacg ctggtttttt acgaacttag tgaaataatg tagaatataa
     2941 aattataatt ttatattgga gcacatgtgt taatgatcaa ccccgggata ctaaaaatat
     3001 agacattatt aaacttgctt agcctcgcat tgcctttacc tgttaaagat gcatcgagaa
     3061 aattgagtct gaagttctca tcctttatct gattttccag tcccgaaact ttggccttag
     3121 cattacctga gatccgtccg ttactatcta ttttgtaggt aaaaaaatac gaatgggaaa
     3181 atttcctcgg tgtatcttca tcagttaagc caacaatcgt aaaactaccg ctaccatctt
     3241 tttttaaaat gactgaaagg ttagcagagt agtataaaga atgatctgtt ttagcatttc
     3301 ttgttaacgt cgacgtacat tcaagagacg ttgtgctacc atcacgagcc ttccagagag
     3361 caaaaaaaca cacctgtagc aatgtgaaaa caagtattaa tataaaatac attttcttat
     3421 tagtcatata atagcacctc tgaacaaatt gcataagttt cgtcattaga ggattttata
     3481 catcttgata aataccgctg cccttcccca agcaaataat gcatactatt tacatcatat
     3541 ataaaatttg cacctggcac acagggtaaa tgctttttcg aaatttcagc ggcctctatt
     3601 tgagatttag cgaggccaat ctcgttaaga ttctctgaaa gatataaatc acaggtgtca
     3661 atcttacctg atttaaccaa tggataatcc gttatactgt aggtatcttt aaaagccacg
     3721 gcaaatattt caataataac aattactacc acaagcgaaa tcaaaatgcg aaaaagagga
     3781 atactccttt tgcgctctac tttttcaaca acatcttctt gtaaatgaga atcatcataa
     3841 gattgtttac ttattgttat gttttcactg atgtaaaagc ctactttggg tatcgtaaga
     3901 atcacatttt cttcaacacc aatattttta aaacttttgc gtaacaggga tatatattgg
     3961 ttcaatgtgt tgtttgacag atcaaatgaa aatctctcca aaacgctatc aatgattgag
     4021 ttcctgtcaa ccgctccacc attactttcc agtaacactg ccaaaataca ccccgccgta
     4081 gctggcaaat ttatctcatc ctgggtattt acattcgaga gcgagcattt gttcggatca
     4141 aaaataatcg cgtcattgat aatgaaaaaa catctccgct ccatgcaggc aacaacacaa
     4201 aataagattt tgtcatttta gcctattcct ggtggaactg acacaagaaa ctaaaataca
     4261 aatataaccc tgaaaaagtc atataaaact tatatccaca gaataaaaaa tcaaacatgt
     4321 cagttttatc aatataaagt atcgcaaggc gaaaagtgat gcaagaatac tcctaagtaa
     4381 atgaaagcgc accacatgaa attatcaaca catgtaattt catcattagg tgctatcgaa
     4441 aataactcag gagctactta agatgaataa tgtcttactc atgagacccg atgactcaga
     4501 atttatgata cctgatgact ggtcagggct aaacgcaaaa ggcattacat acgaacaaga
     4561 taatagagtg acagagtctt ttctgccttt taaatctttt tattgttcac cggtgtgcac
     4621 gacagttgaa ctcatctcac aaaataattg ttttgctatg ggtatccgct accttctgga
     4681 aaagaaaaga gacttaaggc taaatcactt taaagccttc tctcataaat gcgagacaaa
     4741 acctgacgtg ttaattatga ccgggtgcac cgtcgctgat acttatagta tattctcacg
     4801 cattgttgaa gaaaatataa tgaaaacgat gacacctatc gtcatcatta ctgggcatca
     4861 ctcagaagat ctttgttctc tggcatcgaa actgggagga gttaaaataa tatcaccgac
     4921 agaaaagatt gcaaaaatag aaaacgctat caatgattct tttaacagca agcctaaaaa
     4981 aataagattt ttaacagata tactctctcc tcaacaaatt gagaccatga aaatggttgc
     5041 gcagggtaga tccgttaatg agatttcagc cgcaatgaat attagcatca agaccgtgta
     5101 tgcacataaa gttcgtgcta tcaataagct gggaattaag aaaaaagttt atgaagcgct
     5161 tttttataaa gcgctaattc agttcaactg atgtaaaaat caccctcgct taataaataa
     5221 tatattctat gtcttttttt ataagttaaa aaaggcggga tatcaaaccc cgccatgaaa
     5281 ttaactaatc atctgcatta ttctgcgtag atataagcct gggtacggat ggtgtaagag
     5341 tctggctcaa caacctgttc tttcatatgg gaaacgacgc gataggcacc acctgttcca
     5401 gtatttttca gcatcatata acgcttaccg ccaatctctt cggaggcagc tgaaggagga
     5461 tcctgctcaa gcgttgtcag ggaaactttc agtttatggt ccgggttatt gtcaccagaa
     5521 attactgcat ccgctgaagt actgctctcc gcataaatat catccgggaa agttacagca
     5581 tagtcgtggc tctgcccatc agatgcgcca acaatacctg ttgccaaagg cgtccatgca
     5641 gttaacgtac cgctatatac ccctttcact ggttgaatgg tattattaac aacgataggc
     5701 ttggtcaaag taagattagc tgacgcttca gtaccctggc ttactaaagt tcccgcctga
     5761 gaagcaaaag ctgtccccgc cagcatgatt gctaacaatg ttttcttcat tatattttct
     5821 ccatgatttt catttacact gcaaaatgca gtttctttct ttgcatttac gtgctatcgc
     5881 gaaaatgctt ccaccaaata atggaaacat cttcacaaaa ataaattatg agtttcttaa
     5941 tttagaaact ataaacatgt cgccgctatc tggtataacc ctgatgcact tttcttcgca
     6001 ggaagttggt attttgcgat acaggattga tctttagaat aaccccattt cacattaaac
     6061 tcgcc
//
LOCUS       DAJTQY010000164         6058 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.164, whole genome shotgun sequence.
ACCESSION   DAJTQY010000164 DAJTQY010000000
VERSION     DAJTQY010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6058
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.164"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            382..1410
                     /gene="torT"
                     /locus_tag="OO349_003765"
     CDS             382..1410
                     /gene="torT"
                     /locus_tag="OO349_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HCR5868674.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHFTVQASMPLKAKRV
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNALDDPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(1383..2075)
                     /gene="torR"
                     /locus_tag="OO349_003766"
     CDS             complement(1383..2075)
                     /gene="torR"
                     /locus_tag="OO349_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HCR5868675.1"
                     /translation="MPHHIVIVEDKPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDYCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            2162..3334
                     /gene="torC"
                     /locus_tag="OO349_003767"
     CDS             2162..3334
                     /gene="torC"
                     /locus_tag="OO349_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HCR5868676.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHARQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            3334..5874
                     /gene="torA"
                     /locus_tag="OO349_003768"
     CDS             3334..5874
                     /gene="torA"
                     /locus_tag="OO349_003768"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HCR5868677.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRRATAAQAAT
                     EAVISKEGILTGSHWGAIRAMVKDGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYEQLKAKVAAGEVEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAHTLYSENLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGIDAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIPIA
                     RFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVIAI
                     DNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDIFR
                     ELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFDHP
                     QMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGGPG
                     SQKYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFINPQDASARGIRNGDVVR
                     VFNARGQVLAGAVVSDRYAPGVARIHEGAWYDPDKGGEPGALCKYGNPNVLTIDIGTS
                     QLAQAASAHTTLVEIEKYNGTVEQVTAFNGPVEMVAQCEYVPASQVKS"
     gene            5871..>6058
                     /gene="torD"
                     /locus_tag="OO349_003769"
     CDS             5871..>6058
                     /gene="torD"
                     /locus_tag="OO349_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="TorD; involved in the biogenesis of torA; acts on
                     torA before the insertion of the molybdenum cofactor and,
                     as a result, probably favors a conformation of the
                     apoenzyme that is competent for acquiring the cofactor;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HCR5868678.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLTAAVNDLENSIAT"
BASE COUNT         1471 a         1516 c         1679 g         1392 t
ORIGIN      
        1 tccatttcgg tcccagttag gacgctttct catcaagcca gtccgcccac cactgcatca
       61 tctccctacg cttatcgaga tactgagcat ggttgtaaat tcaattaacc cagtgcgttg
      121 acaaaaaatt agcgcaagaa gacaaaaatc accttgcgct aatgctctgt tacaggtcac
      181 taataccatc taagtggttg attcatagtg actggatatg ttgtgttttg tcgcgttttt
      241 cagtctatta tttaatcatt aagtaatcta atgctatgat ttttaaatta tttatgtgat
      301 tttgtcatcc tttaggtgaa aaaggttgag tcgcaaagtg gaatgcatct agcataaagc
      361 cttattattg atgaggctat catgcgcgta ctgctatttt tacttctttc ccttttcatg
      421 ttgtcggcat tttcggctga taacctgttg cgctggcatg atgcgcagca tttcacggtg
      481 caagcctcta tgccgcttaa agccaaacgc gtatggaaac tgtgcgcgct ttatcccagc
      541 ctgaaagatt catactggtt atcgttgaac tatggtatgc aggaggctgc tcgccgctac
      601 ggtgtggatt taaaagtgct ggaggcaggc ggctacagcc agttggctac ccagcaagca
      661 caaatcgacc agtgtaaaca gtggggcgca gaggccattt tgctcggtag tagcacgacg
      721 tcatttcccg acctgcaaaa gcaggtagca aatctgccgg tgatcgaact ggtaaatgct
      781 cttgatgatc cccaggtgaa aagccgcgtt ggtgtaccct ggtttcagat gggctatcaa
      841 cctgggcgat atttggtgca atgggcgcac ggtaaaccac tgaacgtgct gctgatgccc
      901 ggacccgata acgccggggg cagtaaggag atggtcgagg gttttcgcgc agccattgcc
      961 ggaagcccgg tgcgtattgt tgatattgcg cttggtgata acgatattga aatccagcgc
     1021 aacctgttgc aggagatgct ggaacgccac ccagaaatcg acgtcgttgc cggaacggcc
     1081 attgcggcag aggcggcaat gggtgaaggg cgtaacctga aaacgccgct taccgtggtg
     1141 tcgttttatc tttcacatca ggtgtatcgc gggctgaagc ggggaagagt gattatggct
     1201 gccagcgatc aaatggtctg gcagggggaa ctggcagttg agcaggccat caggcaatta
     1261 caggggcaat cggtgtctga taatgtcagc ccaccgattt tagttctgac gccgaaaaat
     1321 gccgaccgag aacatatccg ccgctcgctg tcaccggggg gatttcgtcc ggtctatttt
     1381 tatcagcaca catcagcggc taagaaataa ccttcaccat gttgcgtcac cagtaaatcc
     1441 gcgctgagtt tatgacgtaa acgacgaatt aacacatcga cggtgcgcag gtcagggttt
     1501 tccacccgac gcgcagaaag catacgcagc agacgctcac ggctgagaat ttcgcccgga
     1561 ttagtcacaa atgccaccaa catttcatac tctgcgcggg tcagtttaat cggctcgcca
     1621 tcccgctcca gcgtatggcg cgacacattc aggcaataac ccgcaaagcg atagcagtag
     1681 tcctgagtgt gcggttgagc ttgtcgcgcg aggtcgattc gccagagcag atttttcacc
     1741 cgtactacca gttcgcgcag ttccagcggt ttggtgacgt aatcgtctgc gcccatttcc
     1801 agcccaacaa tacggtcaat ccgatcgctg cgtccggtaa ccagaataat ccccaccgtt
     1861 gagcgttctc gcagggcgcg ggttaacatc aggccatttt catcgggtaa gttgatatcc
     1921 agcagaatta aatctaccgg ctgattctgc ataatttccc gtagcccggc accgctcgcc
     1981 gtaacggaaa cggtataccc ctcctgagtg aagtaggatt gtaatcgcgc ctgggtaacc
     2041 ggcttatctt caacaataac aatgtgatgt ggcatcagag ggttttactc attctgttca
     2101 tatctgttca tatttgctca ttaagatcgc ttcactaaac cataattcta caggggttat
     2161 tatgcggaaa ctctggaacg cgctacgccg acccagtgct cgttggtcgg tactggcgct
     2221 ggtcgcaatt gggattgtga ttggcattgc gctgattgta ttgccacacg ttgggatcaa
     2281 agtcaccagc acaaccgaat tttgtgtcag ttgccacagt atgcaaccgg tgtatgaaga
     2341 atataaacag tcggtgcatt tccagaacgc ctccggcgtg cgagctgaat gccatgactg
     2401 tcatatcccg ccggatattc caggcatggt gaagcgcaaa ctggaagcga gcaacgacat
     2461 ttaccagacc tttattgccc actccattga tacacctgaa aaattcgaag ccaaacgcgc
     2521 ggaacttgcc gagcgtgaat gggcgcgaat gaaagaaaac aactcggcaa cctgtcgctc
     2581 ctgccataac tacgatgcga tggatcatgc caggcagcat cctgaagcgg cacgtcagat
     2641 gaaggtggca gcgaaagata atcaatcctg catcgactgt cataaaggta ttgcccacca
     2701 gttaccggat atgagtagcg gcttccgtaa gcagttcgat gagctgcgcg ccagtgctaa
     2761 tgacagtggt gacacgctgt actctattga tattaagcca atttatgcgg cgaaaggcga
     2821 taaagaagcc tctggttctc tgctgcctgc ttcggaagtg aaagtcctta aacgtgacgg
     2881 cgactggctg caaattgaaa ttaccggctg gacggaaagc gccggacgtc agcgtgtact
     2941 cacccaattc ccaggtaaac gcatctttgt tgcctcgatt cgtggtgatg tgcagcagca
     3001 ggtgaaaacg ctggagaaaa ccaccgttgc cgacaccaat accgagtgga gcaagttgca
     3061 ggccaccgcg tggatgaaga aaggcgacat ggtgaacgat atcaaaccga tctgggccta
     3121 tgcggattcg ttgtacaacg gcacctgtaa ccagtgccac ggcgcaccgg aaatcgccca
     3181 ctttgacgct aacggttgga tcggcacgct caacggcatg attggcttta ccagtctcga
     3241 taaacgtgaa gaacgcactt tgttgaaata tctgcaaatg aatgcgtctg acaccgcagg
     3301 taaggctcac ggcgataaga aggaagaaaa ataatgaaca ataacgatct ctttcaggca
     3361 tcacgtcggc gttttctggc acaacttggc ggcttaaccg tcgccgggat gctggggccg
     3421 tcattgttaa cgccgcgccg tgcaactgcg gcgcaagcgg cgactgaggc tgtcatctcg
     3481 aaagagggca ttcttaccgg gtcgcactgg ggggctatcc gcgcgatggt gaaggatggt
     3541 cgctttgtgg cggcaaaacc gttcgaactg gataaatatc cgtcgaaaat gatcgccgga
     3601 ttgccggatc acgtacacaa cgcggcacgt attcgttatc cgatggtacg cgtggactgg
     3661 ctgcgtaagc gccatctgag cgacacctcc cagcgcggtg ataaccgttt tgtgcgcgtg
     3721 agctgggatg aagccctcga catgttctat gaagaactgg aacgcgtaca gaaaactcac
     3781 gggccgagtg ccttgctgac cgccagtggt tggcaatcga cggggatgtt ccataacgct
     3841 tcggggatgc tggcgaaagc tattgccttg catggtaata gcgttggtac gggcggagat
     3901 tactctaccg gtgctgcgca ggtgatcctg ccgcgcgtag tcggttcaat ggaagtgtat
     3961 gaacagcaaa cctcctggcc gctggtattg cagaacagca aaaccattgt gctgtggggc
     4021 tccgatttgc tgaaaaacca gcaagcgaac tggtggtgcc cggatcacga tgtttatgaa
     4081 tattacgagc agctaaaagc gaaagtcgcc gccggtgaag ttgaggtcat cagcatcgat
     4141 ccggttgtca catccaccca tgagtatctg gggcgcgagc atgtgaagca cattgcggtt
     4201 aacccgcaaa ctgacgtgcc gctgcaactg gcgctggcac atacgctgta cagtgaaaac
     4261 ctgtacgaca aaaacttcct cgctaactac tgtgtgggtt ttgagcagtt cctgccgtat
     4321 ctgctgggtg agaaagacgg tcagccgaaa gatgccgcat gggctgaaaa actgaccggc
     4381 attgatgccg aaaccattcg tgggctggcg cggcagatgg cggcgaacag aacgcaaatt
     4441 attgctggct ggtgcgtgca gcgtatgcag cacggtgaac agtgggcgtg gatgattgtg
     4501 gttctggcgg cgatgctggg gcaaattggc ctgccaggtg gtggttttgg ctggcactac
     4561 aacggcgcag gcacgccggg gcgtaaaggc gttattctga gtggtttctc cggctctacg
     4621 tcgattccgc ctgttcacga caacagtgac tacaaaggct acagcagcac tattccgatt
     4681 gcccgtttta tcgatgcgat cctcgaaccg gggaaagtga tcaactggaa cggtaaatcg
     4741 gtaaaactgc cgccgctgaa aatgtgtatt tttgccggaa ctaacccatt ccatcgccat
     4801 cagcagatca accgcattat tgaaggctgg cgcaagctgg aaacggttat cgccatagat
     4861 aaccagtgga cctcaacctg ccgctttgcc gatatcgtgc tgcctgcgac cacgcagttt
     4921 gagcgtaacg atctcgacca gtacggcaac cactccaacc gtggcattat cgccatgaaa
     4981 caggtggtgc cgccgcagtt cgaggcgcgc aacgactttg atattttccg cgagctgtgc
     5041 cgtcgcttta atcgtgaaga agcctttacc gaagggctgg acgaaatggg ctggctgaaa
     5101 cgcatctggc aggaaggtgt acaacaaggc aaaggacgcg gcgttcatct gccagcgttt
     5161 gatgacttct ggaataacaa agagtacgtc gagtttgacc atccgcagat gtttgttcgc
     5221 caccaggcat tccgcgaaga tccggatctc gaaccgctgg gcacgccgag tggcctgatt
     5281 gagatctact cgaaaaccat cgccgatatg aactacgacg attgtcaggg gcatccgatg
     5341 tggtttgaga aaatcgaacg ctcccacggt gggcccggct cgcagaagta tccgttgcat
     5401 ctgcaatctg tgcatccgga tttccgactt cactcgcagc tatgtgagtc ggaaacgctg
     5461 cgtcagcaat atacggtagc gggtaaagag ccagtgttca ttaacccgca ggatgccagc
     5521 gcgcgcggta ttcgtaacgg tgatgtggta cgcgtcttta acgctcgcgg tcaggtgttg
     5581 gcaggagcag tagtttctga ccgctatgca cccggcgtgg cgcgaattca cgaaggggca
     5641 tggtacgatc cagataaagg cggcgagcct ggtgcgctgt gcaaatacgg taaccccaac
     5701 gtgctgacca tcgacatcgg gacttcgcag ctggcgcagg cggccagtgc gcacactacg
     5761 ctggtggaaa ttgagaagta caatggaaca gtggagcagg tgacggcgtt taacggcccc
     5821 gtggagatgg tggcgcagtg cgaatatgtt cccgcgtcgc aggtgaaatc atgaccacgc
     5881 tgacagcaca acagattgcc tgtgtttacg cctggctggc gcagttgttc tcccgtgaac
     5941 tggacgatga acaactgacg caaatcgcca gtgcgcagat ggctgaatgg ttttcgttgc
     6001 tgaaaagcga accgccgctc actgcggcgg tgaatgatct ggaaaacagt attgccac
//
LOCUS       DAJTQY010000165         6041 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.165, whole genome shotgun sequence.
ACCESSION   DAJTQY010000165 DAJTQY010000000
VERSION     DAJTQY010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6041
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.165"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(<1..218)
                     /locus_tag="OO349_003770"
     CDS             complement(<1..218)
                     /locus_tag="OO349_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5868679.1"
                     /translation="MTAYLYRMPVGIAGAISRPQDLTVEPVVLKSDNAFAAYGLAGKY
                     DADGFFVPLADGDTADKVKGIYVRPYPTT"
     gene            complement(223..1443)
                     /locus_tag="OO349_003771"
     CDS             complement(223..1443)
                     /locus_tag="OO349_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HCR5868680.1"
                     /translation="MKYFFNTRLGETRYQLADGSLLCKDVPIGRTGKQLYGAADLPNL
                     KPDKLGEIVVTRSPEQVFHPATLASFEGMSITILHPEDENGNVRLVNPENWKELAVGH
                     LQNVRRGTGDHSDLMLADLIVKDENAIQLIEDGLREVSCGYDAEYEQTEPGKAEQVDI
                     TGNHVALVPKGRAGNRCAIGDRDTMANQKKSWWTRMRTAIKTGDADTMNELVESAPAS
                     VTGDEGDLPQGVNLNINLSPQQPLPDKVPEMGGGPTGDSDDDLKTLLKALLAKLEGNA
                     TGDNDNKPDDNPTGDGEDDEEETTITGDSAWRAEVIVPGIDLSRKMKPTVFKREVLAS
                     ADKTLVRQVVGDADIRKLPKQSVDMAFNAVSEIAKGRNTRSTTGDAQRPNMGMTSIAS
                     LNKQNAEFWSNRKG"
     gene            complement(1458..2195)
                     /locus_tag="OO349_003772"
     CDS             complement(1458..2195)
                     /locus_tag="OO349_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HCR5868681.1"
                     /translation="MATNRQSRQAEIRYRTSLRQIARAVGDIVNGRYDGSNDSVTEIM
                     DALERYSEIITPWATKVAENFTADIARQNEKQWRQHSRNISAELRNMVDRAPVGQVMK
                     SIVAEQIKYIKSLPLEAADRVYDIQNKAIEAVVAGGRAEPFAKEIAASGDVSRSRANL
                     IARTELGRATGALDQARALSIGSNGYIWRTAEDGDVRHSHREMEGKFVEWGKPPTLDG
                     MTGHAGELPNCRCYKEIVFPNPHSYLA"
     gene            complement(2083..3549)
                     /locus_tag="OO349_003773"
     CDS             complement(2083..3549)
                     /locus_tag="OO349_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
                     /protein_id="HCR5868682.1"
                     /translation="MARNKQATRRTAQATADGYENFVARVGMQTPNQHSASTYRANFT
                     SRNRMLVEWSYRSSWIIGEAVDAIPDDMTRKGIRITSEIDAKDRGILESQLDELQIWD
                     ALNDVLKWSRLYGGAVGFIMIEGQAPMTPLRLETIGEGKFKGILPLDRWMINPVLTRR
                     IKEMGPDLGKPEFYDVVTTATGIPAWRIHHSRLIRFDGVTLPFQQKMTENEWGMSVVE
                     RIWDRLTAFDSATVGAAQLVYKAHLRTYSVEKLRELIALGGPAFEALLKNIDLIRQFQ
                     SNEGMTLMDSRDKFETHQYSFSGLDDILSQFAEQISGAVGIPLVRLFGQSPKGFSTGD
                     ADLANYYDRVSSLQERRLRLPMRRILDIMHRSELGKPLPDDFTFEFNPLWQMSDVDRS
                     TVAVNTTNAISTALGDGLMTRKAAMTDLRENSDVTGIGASITDEDIENAEDEAPPGIG
                     ELGDKPPEPPGGDPISNEPTADSAGGRGYRKWALRWFK"
     gene            complement(3549..5171)
                     /locus_tag="OO349_003774"
     CDS             complement(3549..5171)
                     /locus_tag="OO349_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerL protein"
                     /protein_id="HCR5868683.1"
                     /translation="MPLPFPFDFKHPDYQMVFEWRMERLQRIRQNPEILPALKQFYRT
                     NPAQFIIDWGMTTDPRNIDYGLPVTIPFLLFPKQEEWIHWIMERWGNRENGITEKSRE
                     MGLSWTAIGLACSLCLFNKEMVIGFGSRKEEYVDSTGDPKALFWKARKFVETLPVEFR
                     GSWDEKKHAPYMRVEFPETGAVIKGEAGDNIGRGDRTTLYLVDESAFLQRPLLIDAAL
                     SQTTRCRIDLSSVNGMANPFAQKRHGGKIPVFTFHWRDDPRKDEEWYRRECEKIDNPV
                     VVAQELDLNYSASAEGVLIPSEWVQAAVDAHIKLGIQPTGKRLGAMDVADEGRDKNSF
                     STRHGFLLENVREWSGVGSDIYQSVEKVFGFCEQDNLEEFRFDEDGLGAGVRGDARAI
                     NELRNAARRPSILATPFRGSGAVFDPDDEAVRGDNGQAARLNKDFFANAKAQSWWRLR
                     KLFQNTWRAVVEGMDYNPDEIISISSSMALKDKLIIELSQPTYSINGVGKIVIDKQPD
                     GTRSPNLADSVMISYAPMNSALNIWELLGRQA"
     gene            complement(5174..5746)
                     /locus_tag="OO349_003775"
     CDS             complement(5174..5746)
                     /locus_tag="OO349_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001703551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HCR5868684.1"
                     /translation="MFRRCDLKQLTPKQELFCREYLKDLNATQAAIRAGYSEKTARAT
                     GSENLSKPDIADRIAELKAERNEEVGIDAAYVLRRLTEIDQMDVLDILLANGELKQIK
                     DWPKVWRTTLSGMDVVEMASADSAALLKKIKWPDKVKNLELLGRHVSVQAFKDNVKNE
                     VTGADGGPVRTEITNLTPEQAAEAYRKMMG"
     gene            complement(5730..>6041)
                     /locus_tag="OO349_003776"
     CDS             complement(5730..>6041)
                     /locus_tag="OO349_003776"
                     /inference="COORDINATES: protein motif:HMM:NF024784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR5868685.1"
                     /translation="KDLVVRHKCDNPSCVNPDHLIIGTVADNNRDRSRRGRNSYPDRS
                     GEKNGMAKLKECDVICIRREYVRGSVGNGLRSLAKRYGVSTTMIADIVNHRNWSNVSE
                     V"
BASE COUNT         1269 a         1720 c         1535 g         1517 t
ORIGIN      
        1 gtggtcggat aagggcgcac gtagatcccc ttcaccttgt ctgcggtatc gccatctgcc
       61 agcggcacga aaaaaccgtc agcatcgtat ttaccagcca gcccataggc agcaaaggcg
      121 ttatcggatt taaggaccac cggttcgacg gttaagtcct gcgggcgaga gatagccccg
      181 gcaatgccaa caggcatccg gtacagatat gcagtcattg gattatcctt tgcggttaga
      241 ccagaattca gcgttttgtt tgttcaggga agcgatgctg gtcatgccca tgtttggacg
      301 ttgtgcatcg cccgtggtgc tgcgggtgtt tcgccctttg gcaatctcag acacagcgtt
      361 aaacgccata tcgaccgatt gcttaggcaa tttgcggata tccgcatcac cgacaacctg
      421 gcgaaccagc gttttgtcag cagaagccag aacctcgcgt ttgaacacgg tcggtttcat
      481 cttacggctc agatcgatac ccggaacgat aacttcggca cgccaggctg agtcaccagt
      541 aatcgtggtt tcctcttcat cgtcctcgcc gtcaccggtc ggattatcgt caggtttatt
      601 atcgttatcg cccgtggcat ttccttccag cttagccagc agggctttca gtaatgtttt
      661 gaggtcatca tcactgtcgc cggttggacc tccgcccatc tctggtactt tgtccggtag
      721 cggttgctgc ggggacaggt tgatgttgag attaacgccc tgcggcaaat ccccctcatc
      781 tcctgtaacc gatgcgggag ccgactccac cagttcgttc atggtgtcag cgtcacccgt
      841 tttgatggcc gtgcgcatgc gggtccacca gcttttcttt tgatttgcca ttgtgtctct
      901 gtctccaatt gcacaacgat ttccggctct gcctttgggg acaagagcca catggtttcc
      961 ggtaatatcg acctgctcgg ctttacctgg ctcggtctgc tcgtactccg cgtcatagcc
     1021 gcacgacact tcacgcaggc catcttcgat aagctgaatg gcgttttcgt ctttgacgat
     1081 aaggtcagcc agcatcaaat cagagtggtc acccgtcccg cgccgcacat tctgaagatg
     1141 cccgacagca agctctttcc agttctcggg atttaccagc cgcacattcc cgttttcatc
     1201 ttcaggatgc aggatcgtga tgctcatccc ttcgaatgag gcgagcgtgg ccggatggaa
     1261 tacctgctca ggagagcgcg ttacgactat ctcaccgagc ttgtcgggtt tgaggtttgg
     1321 cagatcggca gcgccgtaga gctgtttacc cgttcgacct atcggcacgt ctttgcacag
     1381 cagcgagccg tcagccagct gatagcgggt ttcccccagc cgggtattga aaaaatattt
     1441 catggtttac ctgcgattca ggcgagataa gaatgagggt tgggaaaaac gatttcttta
     1501 taacagcgac aattcgggag ctcgccagcg tgaccggtca tgccatcaag cgttggaggt
     1561 ttgccccatt cgacaaactt accttccatc tcccgatgag aatgccggac gtcgccatct
     1621 tcggctgtac gccagatata accattcgag ccgattgaca gcgcacgcgc ctgatcgagc
     1681 gcgccggttg cacgtccaag ctcggtacgg gcgataaggt tcgctcgtga gcgtgacacg
     1741 tcaccggacg ctgctatctc tttcgcgaat ggttcagcgc ggccaccagc cacaacggcc
     1801 tcgatggcct tgttctgaat gtcatacacc cgatcggcgg cctcaagagg cagtgacttg
     1861 atatacttaa tttgctcggc gacgatggat ttcatcacct ggcctaccgg ggcgcggtcg
     1921 accatgttgc gcagctctgc gctgatgttc cggctgtgct gacgccactg cttttcattc
     1981 tggcgtgcaa tgtcggcggt aaagttctca gcaaccttcg tcgcccaggg ggtgatgatt
     2041 tcgctgtagc gctccagcgc atccattatt tcggtaacgc tatcatttga accatcgtag
     2101 cgcccattta cgatatcccc gaccgcccgc gctatctgcc gtaggctcgt tcgatatcgg
     2161 atctccgcct ggcggctctg gcggtttgtc gccaagttcg ccgatgcctg gcggcgcttc
     2221 gtcttcggca ttctcgatat cctcgtcggt aatggatgcc ccgatgccgg tgacgtcaga
     2281 gttttcgcgc aggtcggtca tcgccgcctt acgcgtcatc aatccgtcgc ccagcgcggt
     2341 actaatcgcg ttggtggtgt ttacggccac cgttgatcgg tcaacgtctg acatttgcca
     2401 tagcgggtta aactcaaacg tgaaatcgtc cggcagcggc tttccgagtt ccgagcggtg
     2461 cataatgtcc agtatccggc gcatcggcag ccgtaagcgg cgctcctgca atgagctcac
     2521 ccggtcgtaa tagttggcga ggtctgcatc accagtagag aagcctttcg gggattgacc
     2581 gaacaaccgc accagcggga taccaacggc accgctaatc tgctcagcaa actgcgaaag
     2641 aatgtcatcc agaccactaa agctgtactg gtgggtttcg aacttatccc gcgagtccat
     2701 gagcgtcata ccttcattgc tctggaactg gcggatcagg tcgatgttct tcagcaacgc
     2761 ctcgaacgcc gggcctccaa gcgcgataag ctcgcgcaac ttctccacgc tataggtacg
     2821 cagatgtgct ttgtagacca gctgcgccgc gccgacagtg gcgctgtcga acgcagtaag
     2881 ccgatcccag atacgctcta caaccgacat tccccattcg ttttcggtca tcttctgctg
     2941 gaatggcagc gtcaccccgt cgaagcgaat caggcggctg tgatggatgc gccaggccgg
     3001 gatgcccgtt gcagtggtca ccacgtcgta aaactcaggt ttgccgagat ctggccccat
     3061 ctctttaatg cggcgggtca ggaccgggtt aatcatccag cggtcgagcg ggagaatgcc
     3121 cttaaacttg ccttctccaa tggtttcgag ccgcagcggg gtcattggtg cctgcccctc
     3181 gatcatgatg aagccgaccg cgccgccgta gaggcgagac catttcagca cgtcgttcag
     3241 cgcatcccag atttgcaact catccagttg tgattcgaga atgccacgat cttttgcatc
     3301 aatttccgaa gtgatgcgaa tgcctttgcg ggtcatatca tccgggatag catcgaccgc
     3361 ttcgccgata atccaggacg aacgatagga ccattccacc agcatgcggt tacgactggt
     3421 gaaattagcc cggtaggtgg atgctgagtg ctggttaggt gtctgcatcc ccacgcgggc
     3481 aacaaagttc tcatagccat cagcggtggc ctgtgccgtt cgccgcgtgg cttgtttgtt
     3541 tcgtgccatc aggcctgtct ccctagcagc tcccagatgt tcagggctga attcattggc
     3601 gcgtagctga tcatcaccga gtcggcaagg tttggcgacc gggttccatc aggctgttta
     3661 tcaataacga tttttcccac accattaatg gaataggtcg gctgcgaaag ctcgatgatg
     3721 agtttatctt tgagtgccat gctactgctg attgagatga tttcgtccgg gttgtaatcc
     3781 ataccttcaa ccacggcgcg ccaggtattc tgaaaaagtt tacgtaaccg ccaccagctc
     3841 tgggctttgg cgttagcgaa gaagtccttg ttcaggcggg cggcctgtcc gttgtcaccg
     3901 cgcaccgctt cgtcgtccgg atcaaatacc gcgccactac ctcgaaacgg tgtggcgagt
     3961 attgacggtc ggcgcgcagc gttacgcagt tcgttgatag cgcgtgcatc gccgcgaacg
     4021 ccagcgccca acccgtcctc gtcaaagcga aactcttcga ggttgtcctg ttcgcaaaag
     4081 ccgaaaacct tctcgacgga ctgataaatg tcgctgccca caccggacca ttcccgcaca
     4141 ttctccagga ggaagccatg acgggtggaa aaggaatttt tgtccctgcc ttcgtcggcg
     4201 acgtccatcg cgcccagtcg cttgcctgtt ggctggatgc ccagcttgat atgcgcgtcg
     4261 acagcagcct gtacccattc ggatggaatc aggacgcctt ccgctgatgc actgtagttc
     4321 aggtcaagtt cctgtgccac caccaccgga ttgtcgattt tctcgcattc cctgcgatac
     4381 cactcttcat ccttgcgagg atcatcccgc cagtggaatg tgaataccgg tatcttcccg
     4441 ccatgacgct tctgagcgaa cgggttcgcc atgccgttaa ctgaactcag gtcaatacgg
     4501 caacgcgtcg tttgtgacaa cgccgcatca atcagcagag gacgctgaag gaatgcagac
     4561 tcatcaacca gataaagcgt ggtacggtca ccacggccaa tattatcgcc agcctcacct
     4621 ttgataacgg caccagtttc aggaaactca acgcgcatgt atggtgcgtg cttcttttcg
     4681 tcccacgaac ctcgaaactc tacaggtagt gtttccacga acttgcgcgc cttccagaac
     4741 aatgctttcg ggtcaccggt gctgtcgacg tattcctctt tacgggagcc gaaaccgata
     4801 accatttctt tgttgaagag acaaagcgag catgccagcc cgatcgcggt ccaactgagc
     4861 cccatttcac gggatttttc ggtaatacca ttctcccgat taccccagcg ttccataatc
     4921 cagtggatcc actcctcctg cttagggaag agtaaaaacg gaatggtcac cggcaggcca
     4981 taatcaatat tacgcgggtc cgttgtcatg ccccagtcga tgatgaactg agccggattg
     5041 gttcggtaaa actgttttag tgcaggcaat atttcaggat tctggcgaat gcgctgtagg
     5101 cgttccatcc gccattcaaa aaccatctgg taatcaggat gtttaaaatc gaaggggaat
     5161 ggtaacggca tacttagccc atcatttttc tatacgcctc tgcagcctgc tccggcgtta
     5221 agttggtaat ttctgttctg actggtcctc cgtcagcacc agtcacttca tttttgacat
     5281 tgtctttaaa cgcctgaaca gaaacatgac gcccgagcaa ctcaaggttt ttaaccttat
     5341 caggccattt gattttcttc aggagagcag cactatctgc tgatgccatc tcgacgacat
     5401 ccattcctga tagcgttgta cgccacacct taggccagtc tttaatctgc ttcagttcgc
     5461 cattggcaag cagaatgtca agcacgtcca tctgatcgat ttcagtcagc cgtcgcaata
     5521 cataggcggc gtctatacct acctcttcat tgcgttcagc ctttagctca gcaatgcgat
     5581 ctgctatgtc aggttttgac aggttctcgc taccagtggc gcgagcagtt ttctcgctgt
     5641 aacccgccct gatagctgcc tgagtggcgt ttaaatcttt taaatattcc ctgcagaaca
     5701 gttcctgctt aggtgtgagc tgtttcaaat cacacctccg aaacattgct ccaatttcta
     5761 tgattaacga tgtcagcaat catggtagtg gatacgccat atctctttgc taaagaccta
     5821 agcccattac ctacactccc gcgcacatac tcgcggcgaa tgcagatgac atcgcactct
     5881 tttaatttcg ccattccgtt cttttcacct gaacggtcag ggtatgagtt tctgcctcgc
     5941 cttgacctgt cacgattatt gtcagcgaca gtacctataa tcagatggtc aggattcacg
     6001 catgatggat tatcgcattt atgcctaaca acaaggtcct t
//
LOCUS       DAJTQY010000166         6028 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.166, whole genome shotgun sequence.
ACCESSION   DAJTQY010000166 DAJTQY010000000
VERSION     DAJTQY010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6028
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.166"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            86..862
                     /gene="exuR"
                     /locus_tag="OO349_003777"
     CDS             86..862
                     /gene="exuR"
                     /locus_tag="OO349_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HCR5868686.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1207..1869
                     /locus_tag="OO349_003778"
     CDS             1207..1869
                     /locus_tag="OO349_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5868687.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1873..2256
                     /gene="mzrA"
                     /locus_tag="OO349_003779"
     CDS             1873..2256
                     /gene="mzrA"
                     /locus_tag="OO349_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HCR5868688.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2403..2757
                     /locus_tag="OO349_003780"
                     /pseudo
     CDS             2403..2757
                     /locus_tag="OO349_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
     gene            2795..3100
                     /locus_tag="OO349_003781"
     CDS             2795..3100
                     /locus_tag="OO349_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HCR5868689.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3103..3507
                     /locus_tag="OO349_003782"
     CDS             3103..3507
                     /locus_tag="OO349_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HCR5868690.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3497..3796
                     /locus_tag="OO349_003783"
     CDS             3497..3796
                     /locus_tag="OO349_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HCR5868691.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            3982..4278
                     /locus_tag="OO349_003784"
     CDS             3982..4278
                     /locus_tag="OO349_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCR5868692.1"
                     /translation="MKKLEDVGVLVAHILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLLTAFLFHSNFAEGVNSLMFMKNLTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            4348..5334
                     /gene="yqjG"
                     /locus_tag="OO349_003785"
     CDS             4348..5334
                     /gene="yqjG"
                     /locus_tag="OO349_003785"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HCR5868693.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            5627..5995
                     /locus_tag="OO349_003786"
     CDS             5627..5995
                     /locus_tag="OO349_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCR5868694.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTIIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEQE"
BASE COUNT         1524 a         1470 c         1519 g         1515 t
ORIGIN      
        1 tcatcaaatt tatatatgac caatggcata cccatgaata aagtggtatg ataacttcgt
       61 ccgcattcca ctccgggaga cgcatatgga aatcactgaa ccacgccgtt tgtatcaaca
      121 acttgctgct gacctgaaag agcgcattga acagggcgtc tatctggtgg gtgataaact
      181 gcctgccgaa cgctttattg ccgatgaaaa gaacgtcagc cgaacggtag ttcgtgaagc
      241 catcatcatg ctggaagttg aaggctatgt ggaggtgcgt aaaggttcag gtattcatgt
      301 ggtttccaac cagccgcgtc atcagcaggc ggctgacaat aatatggaat tcgccaatta
      361 cggtccgttt gagttgcttc aggctcgcca gctcatcgaa agtaatattg ccgagttcgc
      421 ggcaactcag gtaacgaaac aagacatcat gaaactgatg gccattcagg aacaggcgcg
      481 cggcgaacaa tgcttccgtg attccgagtg ggatttacag ttccacattc aggtagccct
      541 ggcgacgcag aactccgccc tggcggccat cgttgaaaaa atgtggaccc agcgtagcca
      601 taacccgtac tggaaaaaac tacacgaaca cattgattcc cgtaccgttg ataactggtg
      661 tgatgaccac gatcaaattc tcaaggcgtt aattcgcaaa gatcctcatg ctgctaagct
      721 ggcaatgtgg cagcatctgg aaaacaccaa aatcatgtta tttaacgaaa ccagcgacga
      781 tttcgagttc aatgccgacc gctatctgtt cgccgaaaac ccggtagtgc atctcgatac
      841 ggccaccagc ggcagcaaat gaaattcctg tccgacaggc gctgcaatgc gcctgtttgt
      901 ctcgtcggta agcgaaaagt ataaagtgtc agcctgtgta aatcctctcg ccaccctccc
      961 ctgcaatcag caaaatcaga ctccacggac atgtaatttt gataacgaac aacgttgacc
     1021 tttgttacaa ttagattcaa tttgaattta tgcttttgaa tgctttctta tctcacgttt
     1081 taacagggaa tagttcaggc tgtgttgatg tatcaaaccc gcagaacata ccaaaacagc
     1141 aataacattg cggtagtgca tcttttaaaa ccagcgtggc gttaaccgat tcaccaggaa
     1201 taatgaatgg aacttttgac ccaattgctg caagccctgt gggcgcagga ttttgaaacc
     1261 ctggccaatc catcgatgat tggcatgttg tattttgtct tgtttgtaat tttgttcctt
     1321 gaaaatggct tgcttccggc ggccttttta ccgggcgaca gtttactggt attggtcggc
     1381 gtgttgattg cgaaaggcgc gatgggttac ccgcaaacga ttctgctgct gaccgttgcc
     1441 gccagcctcg gttgctgggt cagctatatt caggggcgat ggctgggcaa tacccgcacc
     1501 gtacaaaact ggctatctca tttacccgcg cattatcatc aacgcgcaca ccatcttttt
     1561 cataaacacg gtttatcggc gctgttaatt ggtcgcttta ttgcatttgt cagaacactg
     1621 ctgccgacga ttgccgggtt atcagggctg aataacgcgc gctttcagtt cttcaactgg
     1681 atgagcggtc tgctgtgggt attgatcctg acaactctgg gttacatgct cggcaaaacg
     1741 ccggtatttt taaagtacga ggaccagctg atgtcatgcc tgatgctgct cccggtggtg
     1801 ctgctggtgt ttggcctggc aggttctctg gtcgtgttat ggaaaaagaa atatggaaat
     1861 cgggggtaag ggatgcaaat acctcgcatg tcgcttcgcc agctagcctg gtccggcact
     1921 gttttacttc tggtcggcac gctgttgctg gcctggtccg cggttcgcca gcaagagtct
     1981 acgctggcga ttcgtgccgt tcatcaaggc acaacgatgc cagacggttt ttcgatctgg
     2041 catcaccttg acgctcatgg cattcctttc aaaagtatca cccccaaaaa cgacactctg
     2101 ttaattacat ttgactccag cgaccagagc gccgccgcaa aagcggtcct cgacagaaca
     2161 ttgccccatg gctacatcat tgcgcagcag gacaataaca gtcaggctat gcagtggctg
     2221 acccggttac gggataactc tcatcgcttc ggataacttc ctgttatccg aaacatatca
     2281 ctcactttgg tgatttcacc gtaactgtct atgattaatg aggcggtggt taacactacc
     2341 gcttcgttgt ctggacaatc gttcctttgt aataggtccg aatcacaatg gaaggttcaa
     2401 gaatgaaata ccgcatcgct ttagctgttt ctctctttgc tcttagtgcc ggtagttatg
     2461 ccactaccct gtgtcaggaa aaggagcaaa atatccttaa ggagatcagc tatgccgaaa
     2521 aacaccaaaa ccagaatcgt attgacggtc tgaataaagc cctgagtgaa gtccgggcca
     2581 actgttcaga tagccagctg cgtgccgatc atcacaagaa aatcgcaaag cagaaagatg
     2641 aggtggcgga acgccagcaa gatttagccg aggcgaagca aaaaggcgat gccgatagaa
     2701 cggaaactgg cagaagcgca ggaagagctg aaaaagctgg aagcgcgcga ctactaactc
     2761 acaatagtca ctacttactc acctggagaa aactatgtcg aaagaacaca ctacggaaca
     2821 tctgcgtgct gagttgaaat ccctttccga tacgctggaa gaggtgctta gctcatctgg
     2881 cgagaagtcg aaagaagagt tgagtaagat tcgtagcaaa gcggagcagg cactgaaaca
     2941 gagccgttat cgcctgggtg aaaccggtga tgccatcgcc aaacaaaccc gtgtcgcggc
     3001 ggcgcgtgct gatgagtatg tgcgtgaaaa tccgtggacc ggcgtgggaa ttggcgctgc
     3061 aatcggtgta gtgctcggcg ttctgctgtc gcgtcgttaa ttatggcgga cactcatcac
     3121 gcgcaagggc ccggtaaaag cgttctgggc atcgggcagc gaattgtttc catcatggtt
     3181 gaaatggtgg aaacacgtct gcggctggcg gtggtggagc tggaagagga aaaagctaat
     3241 ctctttcaac ttttactgat gctgggcctg acgatgcttt tcgctgcatt tggtcttatg
     3301 agcctgatgg tgctaattat ttgggcggtt gatccgcaat atcgcctgaa tgcgatgatt
     3361 gccaccaccg tggtgttgct gctactggca ctgattggcg gtatctggac gttacgtaaa
     3421 tcgcgtaaat ccaccttgct gcgccataca cgccatgagc tggcaaacga tcggcaactg
     3481 ctcgaggagg agtcccgtga gcagtaaagt cgaacgtgaa cgacgtaagg cgcaactgct
     3541 tagccagatc cagcaacaac ggctggatct ttccgccagt cgtcgtgaat ggctggaggc
     3601 aacaggcgct tacgatcgtc gctggaatat gctgctaagt ctgcgctcct gggcgctggt
     3661 tggcagtagc gtgatggcga tctggacgat tcgccatcct aatatgctgg tccgctgggc
     3721 cagacgcggt tttggcgtat ggagcgcctg gcgtctggtt aaaacgaccc tcaagcagca
     3781 acagcttcgc ggttaacttc cctctggccg gagcaattcc ggccttatcc ctcaaatttt
     3841 ttgaatattt ttgacagttt tccttgctaa caatcatcat tcaccacgtt tatgattctc
     3901 tccatcgaca gcaacgacgc taatagcgcg cccttgcaca aaaaaacaat cagcagcctg
     3961 agtggcgcag tggagagtat gatgaaaaaa ttagaagatg ttggtgtact ggtagcgcac
     4021 attttaatgc cgattctgtt tattaccgca ggctggggaa aaatcaccgc ttactctggt
     4081 acccaacaat atatggaagc aatgggtgtc ccgggtttta tgctgctgac ggcattttta
     4141 tttcacagca actttgctga aggcgtcaac tcgctgatgt tcatgaaaaa cctgacaatt
     4201 tctggcggat tcctgctgct ggcaattacc ggtccgggcg cgtatagcat cgaccgcctg
     4261 ctgaataaaa agtggtaagc acgcgctata cttaacgata aaaaacgagg aggaagctcc
     4321 tcgtttttgc tattggagga gagaaaaatg ggtcaactga ttgacggcgt ctggcatgac
     4381 acctggtacg ataccaaatc taccggcggt aaatttcaac gttcagcttc cgcatttcgt
     4441 aactggctca ctgccgatgg cgcacctggc cccactggca caggcggttt tatcgcagag
     4501 aaagatcgtt atcatctcta tgtttcactc gcctgcccgt gggcgcaccg cacgctgatc
     4561 atgcgcaaac tcaaaggact ggaaccgttt atttccgttt ccgtagtgaa cccgctgatg
     4621 ctggaaaacg gctggacctt tgatgacagt tttccgggag caaccggaga tacgctctat
     4681 caacatgaat ttctgtatca gctttatctc cacgccgatc cacactacag cggacgagtt
     4741 actgttcccg tgctgtggga caaaaagaac cacaccatcg tcagcaacga atcagcagaa
     4801 atcatccgca tgttcaatac cgcgtttgat gcgctgggcg caaaagcggg tgattactac
     4861 ccaccagccc tgcaaacgaa aattgacgaa cttaacggct ggatttatga caccgttaac
     4921 aacggcgtgt ataaagctgg ttttgccacc agccagcaag cctacgacga ggcggtggcg
     4981 aaagtgtttg aatcgctggc gcgactggag cagattttag gccagcatcg ttacctgacc
     5041 ggcaaccagc taaccgaagc cgatattcgc ctgtggacta cgctggtgcg ttttgatcca
     5101 gtgtatgtga cccacttcaa gtgtgataag caccgcatca gcgattacct gaatctgtat
     5161 ggcttcctgc gcgatatcta ccagatgccg gggatcgccg aaactgtcaa tttcgatcac
     5221 atccgtaatc attacttccg cagccataag accatcaacc ctacggggat tatttcaatt
     5281 ggtccgtggc aggatctcga tgaaccgcat ggacgagatg ttcgcttcgg ttaaaaaaag
     5341 gcaccttacg aggtgcctct gataattagc taagtgcatg ttgttcatgt cggatgcggc
     5401 gtgaacgcct tatcaaacct acaaagcgtt tatattcaat atgttacaat gaccgcgtag
     5461 gccagataag cgtagcgcat ctggcgattt tgcgcttgcc atcagtctta gcgcgccttc
     5521 acggcacttt attaattcct tgaacgaata tttactgcca tttcattcac gtctattctt
     5581 aatttgctgc tttaaaaaca agtgattgag caaaattgag gcaaaaatgg actggtatct
     5641 gaaagtacta aaaaattatg tcggtttccg gggtcgtgcg cggcgcaaag agtactggat
     5701 gtttattctg gtcaacatca tctttacgtt cgtgctgggg ctgctggata aaatgttagg
     5761 ctggcaacgc gctggtggcg aaggcatcct gacgataatc tacggtattc tggtgttttt
     5821 accgtggtgg gcggttcagt tccgccgcct gcacgacacc gaccgctcgg cgtggtgggc
     5881 actgctattc ttaatcccgt ttatcggctg gcttattatc atcgtcttta actgccaggc
     5941 gggtacgcca ggcgaaaacc gctttggacc agatccaaaa ctggaacaag aataatgatt
     6001 aagcaccttg taaaaggtgc ttcaaatt
//
LOCUS       DAJTQY010000167         6016 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.167, whole genome shotgun sequence.
ACCESSION   DAJTQY010000167 DAJTQY010000000
VERSION     DAJTQY010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6016
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.167"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            65..583
                     /gene="mddA"
                     /locus_tag="OO349_003787"
     CDS             65..583
                     /gene="mddA"
                     /locus_tag="OO349_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HCR5868695.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGHWLDLTFMQL
                     QLDERTEPDAIG"
     gene            580..1029
                     /locus_tag="OO349_003788"
     CDS             580..1029
                     /locus_tag="OO349_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR5868696.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMARITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(1030..1263)
                     /locus_tag="OO349_003789"
     CDS             complement(1030..1263)
                     /locus_tag="OO349_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
                     /protein_id="HCR5868697.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(1349..1522)
                     /gene="ortT"
                     /locus_tag="OO349_003790"
     CDS             complement(1349..1522)
                     /gene="ortT"
                     /locus_tag="OO349_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HCR5868698.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            1717..1812
                     /gene="yncL"
                     /locus_tag="OO349_003791"
     CDS             1717..1812
                     /gene="yncL"
                     /locus_tag="OO349_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HCR5868699.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            complement(2214..3023)
                     /locus_tag="OO349_003792"
     CDS             complement(2214..3023)
                     /locus_tag="OO349_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HCR5868700.1"
                     /translation="MKVQLYKFTEDKNKTLTFRWTKKHFEFCMDNKIFLNHKGKKSYK
                     ERNLFLFSKGDKITIEDNVIAEEYSTMPVKNFSSVGAFSFPTCHFSGNIRIGRFCSIA
                     SNVKIMGGNHPLNRFTTHMMTYNGEFDKFAMSEFERSWTLKPFITKPENPIIGNDVWI
                     GNDVVLKGGIAIGDGAVIAANSVVTKDVPPYAIVAGVPAKIIRFRFDSNVIDELLRIK
                     WWNYNYSDLPDNNKCDDINYFVEEMNRLISNGNIQERDYKKFNLSEVFRGL"
     gene            complement(3233..4657)
                     /gene="patD"
                     /locus_tag="OO349_003793"
     CDS             complement(3233..4657)
                     /gene="patD"
                     /locus_tag="OO349_003793"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HCR5868701.1"
                     /translation="MQHKLLINGERVSGEGEKQPVYNPATGDVLLEIAEVSAEQVDAA
                     VRAADAAFAEWGQTTPKARAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINILFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(4679..5473)
                     /locus_tag="OO349_003794"
     CDS             complement(4679..5473)
                     /locus_tag="OO349_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5868702.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            complement(5463..5810)
                     /locus_tag="OO349_003795"
                     /pseudo
     CDS             complement(5463..5810)
                     /locus_tag="OO349_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
BASE COUNT         1566 a         1528 c         1346 g         1576 t
ORIGIN      
        1 gttgcttcgg gctgttttcg atagtattga taacatcatc atcattattt tattgaggcc
       61 agacatgtcc atccgttttg cccgcaaagc cgactgtgct gccattgcgg aaatttataa
      121 ccacgccgtg ttatatacgg cggctatctg gaatgaccaa acggtggatg cagataaccg
      181 cattgcctgg tttgaagcgc ggaccatagc aggttatccg gtgctggtaa gtgaagaaga
      241 tggcgtagtt acgggatatg cctcgtttgg cgactggcgt agtttcgatg gttttcgcca
      301 taccgtggaa cattcggttt atgtccatcc cgatcatcag ggcaaaggtc tggggcgtaa
      361 attgttaagt cgattgattg atgaagcgcg ggattgcggg aagcatgtca tggtcgccgg
      421 gatcgaatcg caaaatcagg cttcgctgca tctgcaccag tcgctgggat ttgtcgtcac
      481 cgcacaaatg ccgcaggtag gcactaaatt tggtcattgg ctggatctga catttatgca
      541 gttgcaactc gacgagcgca ctgaaccgga cgcgattgga tgaatcagtc gctcaccctt
      601 gcttttttga ttgccgccgg aattggtctg gtagtccaga acacgctgat ggcgcgcatc
      661 acccagacct cctctaccat tctcatcgcc atgttgctga actcactggt ggggattgtg
      721 ctctttgttt ctattttatg gtttaaacaa ggcatggcgg ggtttggcga actggtgtcc
      781 agcgtgcgct ggtggacact tatacctggc ttgctgggtt cgttttttgt cttcgccagt
      841 atcagcggat atcagaatgt gggagcggca acaactattg cggtactggt cgccagccag
      901 ctgattggcg gtctggtgct ggatatcttc cgcagtcatg gcgtaccact acgggcgttg
      961 ttcggcccaa tatgtggcgc aattttactt gtggttggcg cctggttggt ggccagacgc
     1021 tcattttaat cagaggatgg tgccgccttt ggttaactgt tcgtggcgcg cttccatatc
     1081 ttctttatgc ttgcgaccgt gatgagcaat agcggtgcgc aaacgctgct gctgggtgta
     1141 gcgatcttcc cgacttaact ccgcgtcatc gctcagggcg attaataatt cgttcatatg
     1201 ggcaatgacg ctctcttcaa tcgcggcatc cacgcgcgcg atgacttcat ccagatgcga
     1261 catactttct ccttagccac ttgtgaaatt tatcgattaa tccaaatgat ataagtctgc
     1321 gtggtgccgt caagccagtg agtaagattt aaaacagtga aaacaacagc gccaccggga
     1381 aactcatcgg ccatgtggcc cccaccagaa aggcgcttaa gaaacggatg cgtttcttat
     1441 cgtgagaaag aaaccaggtg ataagaaatg cgattgctgc cataaccgca taaaaaacaa
     1501 gcatatgttg atagagagac atgtacgggc gtccagccat atgaataaaa cgcgcgcaaa
     1561 tatgcgccat tttttgacgt gcatcaagtt ttttcaccag tttgagaaaa aatcatcaaa
     1621 cagcaacttg cccttatcca tcttcagaat gagtattatt gcggattttc ttaacccgta
     1681 ctatacacat aggggcaatg ataaaaggtg gcaaaaatga atgtttccag tagaactgta
     1741 gtactgataa atgtctttgc tgctgttggt ttgtttactc ttatctctat gagatttggc
     1801 tggtttattt gatgtagtga ttttgcacgc agcacgtact gtcagagaaa tcaactctct
     1861 gacagtacgc aagatcttct catactctcc aagtttgcaa cattatcctt aatatttact
     1921 tctttttgct ggcatctaca ttgaaatgag tagaaaacat ccccctactg tcgtctatac
     1981 aaacagctta ctgattcaaa cggaatcttg ttttcacatt tttctatcac gaatacttta
     2041 tacgcgcggg agtctataaa tgctttatac tttcaaggaa taacgtaaat ataaaaagat
     2101 ggcgtaaagt atatgcatgg caggatgttt gcaacctcct tgatgacgct gccccattga
     2161 aattgcaaac cccgccctgg ttatcttgcc catttatcat gcaaaacaga taattatagg
     2221 cctctgaata cctcagaaag attgaatttt ttatagtccc tctcttgaat atttccattt
     2281 gatatcaatc gattcatctc ttcaacaaaa taatttatat catcacactt attattatcc
     2341 ggaagatctg agtagttata attccaccat tttattctca acagttcatc aataacattt
     2401 gaatcaaacc taaatctgat tatttttgca ggaactcctg caactatggc atatgggggc
     2461 acatctttgg taacaacgga attggcagca ataacagcac catcacctat tgcgatgcca
     2521 cctttcaata caacatcatt tcctatccag acatcatttc ctataatcgg attttcaggt
     2581 ttggtaatga atggtttcaa tgtccagcta cgttcaaatt cactcatagc aaacttatca
     2641 aattcaccgt tataagtcat catgtgagta gtaaaacgat tcaaaggatg atttccaccc
     2701 attattttta catttgatgc aatactacaa aatcgaccta tacgtatatt ccctgaaaaa
     2761 tggcaggtcg gaaatgaaaa agcgccgaca ctggagaaat ttttcacagg cattgttgaa
     2821 tattcttcag caataacatt atcttcaatt gttatcttgt caccttttga gaaaagaaat
     2881 aaattccttt ctttatacga cttcttcccc ttatgattta aaaagatttt attatccata
     2941 caaaactcaa agtgtttttt cgtccatctg aaggtaaggg ttttattttt atcttccgtg
     3001 aatttgtaca attgaacttt cattgttaat acttctactt ataaagtatt agtttaataa
     3061 cgatactcta aagccttatg ctaatcaact gtgacaggat gaaaaatcgc catcaaacat
     3121 ttagcctaat ttttacgcca gttattaatg taatcattct aatacttaac cgccattaaa
     3181 ctcagaacct aaaacgatag tggccgtcct ataacagcca ctaacatcta acttaatgtt
     3241 taaccatgac gtggcggacg acggtgtaat cctccagccc ataaagtgac atatccttgc
     3301 cgtaaccaga aagtttctgc ccaccgtgcg gcatttcact taccagcatg aaatgggtat
     3361 tgacccaggt acaaccatat tgcagccgtg cgctgacgcg atgcgccctg cccacatctt
     3421 tcgtccatac cgaagatgca agtccgtact ggctgtcatt cgcccagttc accacctgtt
     3481 cttcgttgtc gaagggcgta acactcacta ctggaccaaa tacctctttt tgcacgatgg
     3541 catcgtcctg taatgcgcca gccagcagcg tcggcgcata gtaatagcca ttacccttgc
     3601 gcttttcacc gccagtgatc actttgatgt gccctgtcgc tttcgcctct tctactgcct
     3661 tgctgacgcg ttcgagatgc gccagcgagc ttaaaggtcc aagctccgta gactcgtcat
     3721 ccggcgcacc agattttaac gttgccaccg cagcacccag tttttccacc agcgtatcgt
     3781 aaatgccttt ttgcgcgtag atccgacaag ccgcagtaca atcctgtcca gcattgtaat
     3841 agccaaatgt acgtacacct tcgaccactg cttcaatatc cgcatcatca aaaacaatca
     3901 ctggcgcttt gccaccaagt tccatatgag tacgcttaat ggacgacgcg gtatggctga
     3961 tgatgtgctc gccggtggcg atagagcccg tcagcgacac catccgcact ttgggatgac
     4021 cggtcagcgg atcacccacc gttttgcctc tgccaaacag tatgttaatc acgcctgccg
     4081 ggaagatatc tttcgccagc tctgccaact tcaacgcggt cagcggggta atttctgatg
     4141 gtttaagcac tacgcagttc cctgccgcca gcgccggagc aagtttccac gcggccatca
     4201 tcagcggata attccacggt gcgatagaag ccacgacccc caacggatcg cgacggatca
     4261 tcgaagtatg accttcaaga tattcacctg ccgccagacc attcagacag cgcgccgcac
     4321 ccgcgaaaaa gcgaaaaaca tcgacaatcg ccgggatttc atcattgaac gcactatgca
     4381 gcggtttgcc acaattacgg gattccagtt cggcaaaaac ctgaccattt tcttcgataa
     4441 catccgccag tttcagcagg cattccgcac gcgctttcgg tgtggtttgc ccccattcag
     4501 caaatgctgc atctgccgca cgcacagcag catcgacctg ctctgcggat acctcggcaa
     4561 tctccagtaa aacgtccccc gttgccggat tatagacagg ctgtttttcc ccttcgccgc
     4621 taacccgttc tccgttaatc agtaacttat gttgcatagc ttattcctgt atcagtggtt
     4681 attgaccatt gtcgccttcg cgggttagcc accaggcccc caggatcggc aaggttgtta
     4741 ccaacataac cagcagtgcc accacgttgg ttaccggtac atcacgcggt cgcccaagct
     4801 gattgagcaa ccacaacggt aacgttcgtt catgacctgc cgtaaaggtc gtaacgatga
     4861 tttcatcgaa cgacaaggca aacgccagca ttcctcctgc cagtaacgcc gaactgagat
     4921 tcggcaacac tacgtagcgg aaggtttgcc agccattggc cccaagatcc attgacgcct
     4981 caaccagact ccacgaggtg cggcgaaaac gggcgatgac attgttaaac accaccacta
     5041 cgcaaaaagt cgcatgaccg accacgatgg tgaaaaatcc cggctccaga ttgatggttt
     5101 taaaggcggt taatagcgcc agaccagtga caatgcccgg cagcgcaatg ggcagcagca
     5161 ataacagcga aatggcgttt ttgccgaaaa agtctcgtct ccacagcgcg gcagctgcca
     5221 gcgtccctaa caccagcgca attaatgtcg ccagcgccgc cactttaagt gacagcgtca
     5281 cggcatcaag aatatcacta cgctgtgctg ccacgctaaa ccagcgcagc gtcaggccct
     5341 gcggtggaaa actaaacgcc gcatcttcag tgttaaacgc ataggcggcg attatcagga
     5401 tgggaaaatg taggaaaaca acgccgcccc aggccgccag tttgaggaaa aacggtgcgc
     5461 gttcagagtg catcgaacgc tcccagacgt ttcacgaacg ctaggtacag tgcgatgaga
     5521 ataatcggca ccagggtgaa tgccgccgcc atcggcatat tgccaatcgc cccctgctgg
     5581 gaataaacca tattgccgat aaaatatcct ggaggaccaa ccagctgcgg gacgataaaa
     5641 tcgcccagtg tgagtgagaa ggtaaagata gagccagcgg caatacccgg gattgccagc
     5701 ggcagcacca catagcgaaa ggtttgtcgt ggacgtgcgc cgagatcagc cgacgcctgc
     5761 aacaatgacg gcggcaaacg ctcaagcgcc gcctgaacgg gcaggatcat cgagtaggca
     5821 gcctgacggc tgcggcttgt catggtctgc aattaccgtt ataaaaacag gcaatgtcat
     5881 tgtctttcag gtggttatat ggcccgttca gctaaacccc gtaaacgtaa acccgcctct
     5941 caaagttcct gagtcgtgac ccgttcgaat gcgtgctgtg tggcggccgg atggtatacc
     6001 gccgggccat tgcggg
//
LOCUS       DAJTQY010000168         6000 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.168, whole genome shotgun sequence.
ACCESSION   DAJTQY010000168 DAJTQY010000000
VERSION     DAJTQY010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6000
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.168"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            376..1284
                     /locus_tag="OO349_003796"
     CDS             376..1284
                     /locus_tag="OO349_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HCR5868703.1"
                     /translation="MKAPVSLTSFRPQKSLAIAIGVLAVVVLPFLSYYTVNEGERGIL
                     LRYGKIVKVAEPGLGFKIPFMESVEKISTRNQAVVYQGLQAYSRDQQPAQMTVSVSFH
                     IKPSEAGAVYTTYNTIEALKDRLIVRQLPTQLENIFGQYTAISAVQDRTKLVQDLQNA
                     MRKAVVGPVVIDGVQIENIDFSDAYEKSIENRMKAEVAIATRKQNLETEKIQAQIAVT
                     QAQAEADSKLAAAKVEAETIRVRGAAEAETIRLKSAAEAEAIRLRGEALRDNPGLVAL
                     TTAERWDGKLPDTMIPGSTVPFISTK"
     gene            complement(1330..3321)
                     /gene="tkt"
                     /locus_tag="OO349_003797"
     CDS             complement(1330..3321)
                     /gene="tkt"
                     /locus_tag="OO349_003797"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCR5868704.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKALL"
     gene            3599..4357
                     /gene="loiP"
                     /locus_tag="OO349_003798"
                     /gene_synonym="yggG"
     CDS             3599..4357
                     /gene="loiP"
                     /locus_tag="OO349_003798"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HCR5868705.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLHQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRISADGIK"
     gene            complement(4563..5483)
                     /gene="speB"
                     /locus_tag="OO349_003799"
     CDS             complement(4563..5483)
                     /gene="speB"
                     /locus_tag="OO349_003799"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HCR5868706.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(5619..>6000)
                     /locus_tag="OO349_003800"
     CDS             complement(5619..>6000)
                     /locus_tag="OO349_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5868707.1"
                     /translation="VGMNYQLYVRNYSRNTNYEIVATADGLDVLNGKQGSLNNNGYIV
                     NAGDSLVIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGPAQN
                     ALPPCSGQAFPADNNGYAPPPCRK"
BASE COUNT         1469 a         1568 c         1552 g         1411 t
ORIGIN      
        1 attatcctta ttttattttt agggtaccgt ccggcggtaa aaataaaata ctcgtgcggt
       61 ttaatgaaag caattgggta cgcgagaaga aaaaccccac aaaccaaaat aataaaacct
      121 taaataacaa caatatgaaa ttgatatttt taaagtcaac tttgtatttg ttatgttcat
      181 cacaaaatca acattttgtt tgttattgta tttgtgatgc atatctctat ttatttattg
      241 aatgttcgaa aacatacttt tctaatgcaa tgttttttcg ctatacccct ttatagattc
      301 tgaattctca cctgtcggct gttttctcct catctcatgt gacatattcc catactttac
      361 tcaaggaaga ttgtcatgaa agcacctgtt tcattaacct catttcgccc acaaaaatct
      421 cttgctatcg ccattggcgt gcttgccgtg gttgtgctgc catttctctc gtattacacc
      481 gttaatgaag gtgaacgcgg cattttgctg cgctacggaa aaatcgtaaa agttgccgaa
      541 ccggggctgg gttttaagat cccttttatg gaaagcgtgg agaaaatttc cacgcgtaat
      601 caggctgtgg tctatcaggg attacaggcc tacagccgcg accagcaacc ggcacaaatg
      661 acagtatcag tcagcttcca cattaaacct tcagaagcag gcgcggttta taccacctac
      721 aacactattg aagcattaaa agatcgtcta attgtccgtc agttgccaac tcagctggag
      781 aacattttcg gccaatatac cgccatcagc gccgtacagg atcgtacaaa attggttcag
      841 gatctgcaaa atgccatgcg caaagccgtt gttgggccag tggtaattga cggggtccag
      901 attgaaaata ttgatttttc tgacgcttat gaaaagtcga ttgaaaaccg aatgaaagca
      961 gaagtcgcca ttgccacgcg taagcaaaat ctggaaacag agaaaatcca ggcgcaaata
     1021 gcggtaactc aggcccaggc agaagcagac agtaaactgg cggcagccaa agtagaagcg
     1081 gaaactattc gcgtaagagg cgcggcagag gctgaaacta ttcgtttaaa aagtgcggct
     1141 gaagcagaag caatccgcct gcgcggtgaa gctctgcggg ataatcccgg acttgtagcc
     1201 ctcaccactg ccgaacgttg ggacggtaaa ctgcccgata ccatgatccc cggcagcaca
     1261 gttccgttta tctctactaa gtaaaaaaag ggccgctttc gcggtccttc tcacaaaacg
     1321 tcttactgat tacagcagcg cttttgcttt cgcaacaacg ttatcaacag tgaagccgaa
     1381 ctcttcaaac agcagctctg ccggagcaga ttcaccgaag gtggtcatgc cgacgatagc
     1441 gccgttcagg ccaacatact tgtaccagta gtcagcaata cccgcttcta ccgcaacgcg
     1501 tgcagtaacc gctttcggca gtacggattc acggtaagcc gcatcctgct tgtcaaatgc
     1561 gtcggtagac ggcatggaca ccacgcgcgc tttcacgcct tcggcagtca gtttttcgta
     1621 ggcagcaacc gccagttcaa cttcagaacc ggtggcgatg aagatcagtt ccggctgacc
     1681 agcacagtct ttcagcacat aaccaccgcg cgcgatgttt gccagctgct cttcagtacg
     1741 ttcctgctgc gccaggttct gacgggagag gataagcgca gtcgggccgt cctgacgctc
     1801 aacgccgtat ttccacgcga ccgcggattc aacctggtca cacggacgcc atgtagacat
     1861 gttcggggtc acgcgcagag aagcgacctg ctctaccggc tggtgagtcg ggccatcttc
     1921 gcccagaccg atggagtcgt gggtgtaaac catcacctgg cgctgtttca tcagcgcagc
     1981 catacgtacg gcgttacgtg cgtattccac gaacatcagg aaggtggagg tgtacggcag
     2041 gaagccaccg tgcagggaga taccgttagc aatcgcggtc ataccgaact cgcgaacacc
     2101 gtagtggatg tagttacctg cagcatcttc gttgattgct ttagaaccag accacagggt
     2161 caggttagac ggtgccaggt cagcagagcc gccgaggaat tcaggcaaca gcgggccgaa
     2221 cgcttcgata gcattctgcg acgctttacg gctggcgatt ttcgccggat tagcctgcag
     2281 tttagcgata aactctttcg ctttggcgtc gaagtcagac ggcatttcgc ctttcatacg
     2341 gcgggtaaat tcagccgctt cctgcggata agctttcgcg taagccgcaa acttctcatt
     2401 ccatgcagat tctttcgcct ggcctgcttc tttcgcatcc cactgagcat agatttcaga
     2461 cgggatttcg aacggtgcat atttccagcc cagctgttcg cgggtcaggg caatttcagc
     2521 gtcgcccagc ggcgcaccgt gggagtcgtg agtaccggct ttgttcgggg aaccgaaacc
     2581 gatgatggtt ttgcacatca gcagggacgg tttgtcagtc actgcgcgcg cttcttcaac
     2641 tgcgcgtttg atggatgccg cgtcatgacc gtcgatgtcg cgaataacgt gccagccgta
     2701 agcttcgaaa cgcattgctg tgtcgtcggt gaaccagcct tcaacgtgac catcgataga
     2761 aataccgttg tcatcgtaga acgcaatcag tttacccagc ttcagcgtac ccgccagaga
     2821 gcaaacttcg tgggagatgc cttccatcat gcagccgtcg cccatgaagg cgtaggtgta
     2881 gtggtcgaca atgtcgtggc ccggacggtt aaactgcgcc gccagcgttt tttctgcaat
     2941 cgccataccg actgcgttgg caataccctg acccagcgga ccggtggtgg tttccacacc
     3001 agcggtataa cctacttccg ggtggcccgg agttttagag tgcagctgac ggaagttttt
     3061 cagttcttcc atcggcagat cgtaaccggt gaggtgcagc aggctgtaga tcagcatgga
     3121 gccgtggccg ttggacagca cgaagcggtc acggtcagcc caggacggat tctgcgggtt
     3181 gtgtttcagg aaatcacgcc acaggacttc ggcaatgtca gccataccca taggggcacc
     3241 cgggtgaccg gatttggctt tctgtactgc gtccatgctc agcgcacgaa tagcattggc
     3301 aagctcttta cgtgaggaca ttttgactcc agatcggatg atgaagggca cgcccttaac
     3361 gacttgacga cagcgcgttt tgggctacgc cggaaaattt gccaacaatt taccgcaagc
     3421 cgcgcgtcat gtacatggaa catccttttg ccgcttcaga aatctctgga tcatgctcgc
     3481 atgttgcgca atctgctcgc ccgtccgctg cgcttttcct tatactgaga ctgagcgtcg
     3541 attcacctgc aaacggcgca tttttagaat aatcctgacc ttgtgcggaa gagaaaacat
     3601 gaaaattcgc gccttattgg tagcaatgag cgtggcaacg gtactgaccg gttgccagaa
     3661 tatggactcc aacggactgc tctcatcagg agcggaagct tttcaggctt acagtttgag
     3721 tgatgcgcag gtgaaaaccc tgagcgatca ggcatgtcag gagatggaca gcaaggcgac
     3781 gattgcgcca gccaatagcg aatacgctaa acgtctgaca actattgcca atgcgctagg
     3841 caacaatatc aacggtcagc cggtgaatta caaagtgtat atggcgaagg atgtgaacgc
     3901 ctttgcaatg gctaacggct gtatccgcgt ctatagcggg ctgatggata tgatgacaga
     3961 taacgaagtc gaagcggtga tcggtcacga gatggggcac gtggcgttag gccatgtgaa
     4021 gaaagggatg caggtggcgc ttggtacaaa tgccgtgcga gtagctgcgg cctctgcggg
     4081 cgggattgtc ggcagtttat ctcaatcaca acttggtgat ctgggcgaga aattagtcaa
     4141 ttcgcaattc tcccagcgtc aggaagcaga agccgatgat tattcttacg atcttctgca
     4201 ccaacgcggc atcagcccgg caggtcttgc caccagcttt gaaaaactgg cgaagctgga
     4261 agaaggccgt caaagctcaa tgtttgacga ccatcctgca tccgccgaac gtgcccagca
     4321 tattcgcgat cgcatcagcg cggatgggat taagtaaagc ctagtggtgt cgaacgtagg
     4381 tcagataagg cgttcacgcc gcatctgaca ttaatggcac atttgttacc ttgtgcgcat
     4441 tgccggatgc gatgctggcg catcttatcc ggcctacggg tgccgaacgt aggtcggata
     4501 aggcgttcac gccgcatccg acattaatgg cacgttttac ccgtgcgcat cgcatctggt
     4561 gcttactcgc cctttttcgc cgcctgaata tacagcatct ccagcgccag cgtcgccgca
     4621 gccagagcgg tgatttccga ctgatcgtat gccggagcca cttccactac gtccatccca
     4681 acgatgttga gatctttcag gccgcgtacc agtttaatgg cgcgatcgga ggtcaggccg
     4741 ccaatcactg gcgtaccggt tcccggcgcg aaagcaggat ccaggcagtc gatatcaaaa
     4801 gtcaggtaaa ccggcatatc acccacaatc tgtttcacct gggcgataac gtcatccacg
     4861 ctgcgatcgt tcacctggca ggcgtccagc acggtaaagc cgttgtcttt atcaaactcg
     4921 gtacgaatac caatctgcac ggaatgattc gggtcgatca gaccttcttt cggcgcggta
     4981 tagaacatag tgccgtggtc aaattcacaa ccgttcgcat aggtatcggt gtgggcgtca
     5041 aagtgtacca gcgccatttt gccgaaatgc ttcgcatgag cacgcagcag cggcagcgta
     5101 acaaagtggt caccaccgaa ggagagcata cgcttaccgg cagccagcag cttctcggcg
     5161 tgcgcctgca gcttttcgct catctcacgg gcatcgccaa aggcatatac cagatcgccg
     5221 cagtccacga cgttcagacg ctcacgcatg tcgaaattcc acgggaagcg gttgtgttcc
     5281 caggccagat tcgtcgaaac ctgacggatc gctgccggac cgtggcgacc acccgcacga
     5341 ccagaagtgg ccatatcgaa cggcacgcca gtaatcaccc agtctgcatc gctgtcatac
     5401 ggctggaagt tcatcggcag gcgtaaaaaa ccaaaggcat tggaaaccag tgagttatcg
     5461 tattgatgac ctaaggtgct catggatgga ctcctattac aaagtcgata taaaaaaacc
     5521 cttccgcgtc gttaggcccg acgaggaagg gttggatttg cgaaacacaa gtttgtgcta
     5581 attatcgcct taaatgggcg aggcatcaag cgaggttgct atttccgaca cggtggcggc
     5641 gcatagccat tgttatctgc cggaaaggct tgtccagagc acggcggcag cgcgttttgc
     5701 gccgggcctt gtaattcaaa ggcggcaaag ccgattaccc cggtattacg cacatcgcct
     5761 tgcgccgaat tggctgcgta ggaatcagca acgttggcga actgaaaagc cgcttcagta
     5821 tgtttatctt tgcggaatcc tttaatcact aacgaatctc cggcattcac aatatagccg
     5881 ttgttattca gcgaaccttg cttgccgttc agcacatcca ggccatcagc cgtagcaacg
     5941 atctcgtagt tggtgttacg actgtaatta cggacatata attgataatt cattccgact
//
LOCUS       DAJTQY010000169         5901 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.169, whole genome shotgun sequence.
ACCESSION   DAJTQY010000169 DAJTQY010000000
VERSION     DAJTQY010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5901
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.169"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            <1..1207
                     /gene="narU"
                     /locus_tag="OO349_003801"
     CDS             <1..1207
                     /gene="narU"
                     /locus_tag="OO349_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HCR5868708.1"
                     /translation="KIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRWPVF
                     STAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAKQGS
                     ALGINDGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWVPLL
                     AIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFAMLA
                     KTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLFLTL
                     PGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQAQRE
                     AVTETAATLGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLTWLV
                     YGRRKFSQK"
     gene            1289..5029
                     /gene="narZ"
                     /locus_tag="OO349_003802"
                     /pseudo
     CDS             1289..5029
                     /gene="narZ"
                     /locus_tag="OO349_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
     gene            5026..>5901
                     /gene="narH"
                     /locus_tag="OO349_003803"
     CDS             5026..>5901
                     /gene="narH"
                     /locus_tag="OO349_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCR5868709.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCNNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQQIDDYY
                     EPFTFDYEHLHSTPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRN
                     FEAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYD
                     ADRIEEASSTEREVDL"
BASE COUNT         1373 a         1587 c         1588 g         1353 t
ORIGIN      
        1 taaaatcggt tttaatttta ctaccgatca actcttttta ttaaccgcat taccctccgt
       61 ttctggcgca ttattgcgtg ttccctactc ctttatggtg cctatattcg gtggacgccg
      121 atggccggtt tttagtactg caatcctgat tattccttgc gtctggctcg gaattgccgt
      181 gcaaaatccg aatactcctt ttgggatatt tatcgttatc gctttgctat gcggttttgc
      241 aggtgcaaac tttgcttcga gcatgggcaa tatcagtttc ttctttccaa aagccaaaca
      301 agggagcgct cttgggatta atgacggatt aggaaactta ggtgtaagtg taatgcagct
      361 ggttgcaccg ctggtcattt ttgtacctgt atttgccttt ctcggcgtca atggcgtacc
      421 gcaggccgac ggttcggtga tgtcgctggc gaatgccgca tggatttggg taccgctact
      481 ggcgattgcc acgatcgccg cctggtcagg gatgaatgat atcgccagtt cacgcgcctc
      541 aattgccgac cagctccctg tcttacaacg cctgcatctc tggctgctga gcctgcttta
      601 ccttgccacc ttcggttcgt ttatcggttt ttctgcgggt tttgccatgc tggcaaaaac
      661 ccagttcccg gatgtgaata ttctgcgcct ggcgttcttt ggcccattta ttggtgccat
      721 cgcgcggtcg gttggtggtg ctatttccga taaattcggc ggcgtgcggg tgacgttgat
      781 caactttatt tttatggcga ttttcagtgc cctgctgttc cttaccttac cgggcacagg
      841 ctccggtaat ttcatcgcct tttacgccgt atttatgggg ctgtttctga ccgcgggtct
      901 gggaagtggt tctactttcc agatgatcgc cgtcatcttt cgccagataa ccatttatcg
      961 ggtgaagatg aaaggcggta gtgatgagca agctcagaga gaagccgtca ccgaaacggc
     1021 ggcaacgctg ggctttatct cagccattgg cgcagtgggc ggctttttta ttccgcaggc
     1081 atttggcatg tcgctcaata tgaccggctc tccggtcggc gcgatgaaag tgtttttaat
     1141 cttctacatc gtttgtgtgc tgctgacctg gctggtttat ggtcggcgga agttcagcca
     1201 aaaataagag atcgatgcct gatgtacaac catgtcgcat ccgacaaggc atcagatccc
     1261 aattcattcc tggagcagga gtcatgtcat gagtaaactt ttggatcgct ttcgctactt
     1321 caaacaaaag ggcgaaacct ttgccgatgg tcacggacag gtgatgcata gcaaccgcga
     1381 ctgggaggac agctatcgcc agcgttggca gttcgacaaa atcgtgcgtt ccacccacgg
     1441 cgttaactgt acaggctcct gtagctggaa aatctacgtt aaaaatggtc tggtgacctg
     1501 ggaaatccaa cagaccgact acccgcgcac tcgccctgac ctgcccaatc atgaacctcg
     1561 cggctgcccg cgtggcgcaa gttactcctg gtatctttac agcgctaacc gcctgaaata
     1621 cccgctcatt cgtaaacgac tgattgaact gtggcgcgaa gccctcaaac aacacagcga
     1681 tccggtactg gcgtgggcat cgattatgaa cgatccgcaa aagtgcctga gctacaaaca
     1741 agtgcgtggg cgcggcgggt ttatccgctc caactggcag gaactaaacc agctgattgc
     1801 cgccgctaac gtctggacca tcaaaaccta cggcccggat cgcgttgccg gtttctcgcc
     1861 gatcccggcg atgtcgatgg tttcttacgc cgccggaacg cgttatctgt cgctgcttgg
     1921 cggcacctgt taaagtttct acgactggta ttgcgacctg ccgcccgcct cgccgatgac
     1981 ctggggcgag caaaccgacg taccggaatc tgccgactgg tataactcca gctacatcat
     2041 cgcttggggg tctaacgtac cgcagacacg tacgccggac gcccacttct ttaccgaagt
     2101 acgctacaaa ggcactaaaa ccatcgccat tacccctgac tactctgaag tggccaaatt
     2161 gtgcgaccag tggctggcac cgaaacaagg cactgatagc gccctggcga tggcaatggg
     2221 gcatgtgatt ttaaaagagt ttcatctcga taatcccagc gactacttta tcaactactg
     2281 ccgccgctac agcgacatgc cgatgctggt aatgctggag cctcgcgacg atggtagcta
     2341 cgttcccggg cgcatgatcc gcgcatctga cctggtggat ggactgggcg aaagcaacaa
     2401 tccgcagtgg aaaaccgtag cagttaatac cgcaggtgaa ttggtagtgc cgaacggttc
     2461 gattggtttc cgctggggag aaaaaggcaa atggaatctg gaatccattg ccgccggtac
     2521 ggaaaccgaa ttgtcgttaa ccctgctcgg tcaacatgac gctgttgcag gcgtggcctt
     2581 cccctacttt ggcggcattg aaaatccgca ttttcgcagc gtaaaacaca atccggtgct
     2641 ggtgcgccaa ttgcccgtta aaaacctgac gttagtcgat ggcaacacct gtccggtggt
     2701 cagcgtttat gatttggtac tggcgaatta cggcctcgat cgcgggctgg aagatgaaaa
     2761 cagcgcgaaa gattacgctg aaatcaaacc gtacacccca gcctggggtg agcaaattac
     2821 cggcgtaccg cgccagtata ttgaaaccat cgcccgcgaa tttgccgata ccgcccataa
     2881 aacgcatggg cgctcaatga ttatcctcgg cgcaggtgtt aaccactggt atcacatgga
     2941 catgaactac cgcgggatga tcaatatgct gatcttctgc ggttgtgtcg ggcaaagcgg
     3001 tggcggctgg gcacactatg tcggccagga aaaactgcgc ccacaaaccg gctggttgcc
     3061 actggccttt gcgctcgact ggaaccgacc accgcgccag atgaacagca cctcgttttt
     3121 ctacaatcat tccagccaat ggcgctatga aaaagtctct gctcaggagt tactttcacc
     3181 gctcgccgat gccagtaagt acagcggtca tctgattgat ttcaacgttc gcgccgaacg
     3241 tatgggctgg ctaccttctg cgccgcagct ggggcgtaac ccgctcggga ttaaagctga
     3301 agccgacaag gccggattat cccccacaga atttaccgcc caggcgctga aatcgggcga
     3361 tttacgtatg gcctgcgaac aaccagatag cggcagcaat catccgcgta atttgtttgt
     3421 ctggcgctct aacctgcttg gctcctccgg caaaggccac gagtatatgc agaagtatct
     3481 gctggggacc gaaagcggga ttcagggcga ggaactcggt gccagcgacg ggatcaaacc
     3541 ggaagaagtc gagtggcaaa ctgcggcgat tgaaggcaag ctcgacctgc tggtgacgct
     3601 cgacttccgc atgtccagta cctgcctgtt ctccgatatc gttctgccca ccgccacctg
     3661 gtatgaaaaa gacgatatga acacctcgga tatgcatcca tttattcatc cgctttctgc
     3721 ggcggtcgat ccggcctggg aatcacgcag cgactgggaa atctacaaag gtattgccaa
     3781 agcattttcg caagtgtgcg tgggccatct tggcaaagaa accgacgtgg tattacaacc
     3841 actgctgcat gactctccgg cagagctctc acagccgtgt gaagtgctcg actggcgcaa
     3901 aggcgaatgc gatctgatcc cgggtaaaac cgcgccgaat attgtggcgg tggagcgcga
     3961 ctaccctgct acgtatgaac gctttacctc gctcgggcca ttgatggaca aacttggcaa
     4021 tggcggtaaa gggatttcgt ggaataccca ggatgaaatc gatttcctcg gcaaactcaa
     4081 ttacaccaag cgtgatggcc cggcacaggg gcgtccgctg attgacaccg ccattgacgc
     4141 ttcagaagtg attctggcac tggcaccgga aaccaacggt catgttgcag tcaaagcgtg
     4201 gcaggcgctg ggcgagatca ccgggcgcga acatacccat ctggcgctgc acaaagagga
     4261 cgagaagatt cgctttcgcg atattcaggc gcagccgcgt aaaattatct ccagccccac
     4321 atggtccggt ctggaaagcg atcacgtatc ctataacgcg ggatacacca acgttcatga
     4381 gttaattccg tggcgcacgc tgtcgggacg ccagcagctc tatcaggatc atccgtggat
     4441 gcgtgctttt ggtgaaagcc tggtggctta tcgcccgcct atcgacaccc gtagcgtcag
     4501 tgagatgcgc cagataccgc caaacggctt ccaggaaaaa gcacttaact tcctgacgcc
     4561 gcaccagaaa tggggcattc actcaaccta cagtgaaaac ctgctaatgc tgacgctctc
     4621 tcgcggtgga ccgattgtct ggatcagcga aacagatgcc cgagaactga ccattgtcga
     4681 taacgactgg gtggaagtat ttaacgccaa cggcgcgctg actgcccgcg cggtggtcag
     4741 ccaacgtgta ccgccgggca tgaccatgat gtatcacgcc caggaacgca ttatgaatat
     4801 tcctggttcg gaagtaactg gcatgcgcgg cggcattcat aactcggtca cccgcgtttg
     4861 cccgaaaccg acgcatatga ttggcggtta cgcgcagctg gcctggggct ttaactacta
     4921 cggcaccgtc ggctcgaacc gcgacgaatt catcatgatc cgcaagatga agaacgttaa
     4981 ctggctggat gatgaaggtc gcgatcaggt acaggaggcg aaaaaatgaa aatacgttcg
     5041 caagtcggca tggtgcttaa cctcgataaa tgtatcggct gccatacctg ttcggtgacc
     5101 tgtaacaacg tctggaccgg acgcgaaggc atggagtacg catggtttaa caacgtcgaa
     5161 accaaaccgg gcattggtta tccgaaaaac tgggaagatc aggaagagtg gcaaggcggc
     5221 tgggtccgcg atgtgaatgg caagatccgt ccgcgtctgg gcagcaagat gggcgtaata
     5281 accaaaatct tcgccaaccc ggtggtgccg cagcagattg atgattacta cgaacctttc
     5341 accttcgact acgaacattt gcatagcaca ccggaaggca aacatatccc tactgctcgc
     5401 ccgcgttcac tgattgacgg caaacggatg gacaaagtga tctggggccc aaactgggaa
     5461 gaactgctgg gcggcgagtt tgaaaaacgt gcccgcgacc gcaacttcga ggccatgcaa
     5521 aaggagatgt acgggcagtt tgaaaacacc ttcatgatgt acctgccgcg cctgtgcgaa
     5581 cactgcctca atcccagctg cgtggcgacc tgcccaagcg gcgctatcta caaacgcgaa
     5641 gaagacggca ttgtgctgat tgatcaggat aaatgccgtg gctggcgttt gtgcattagc
     5701 ggttgtccgt acaaaaaaat ctacttcaac tggaaaagcg gcaagtcaga aaaatgtatc
     5761 ttctgttatc cgcgaattga gtcaggccaa cccaccgtgt gctcagaaac ctgcgtgggt
     5821 cgcatccggt atctgggcgt gctgctttac gacgccgacc gcatcgagga agcgtcgagc
     5881 accgagcgcg aagttgacct c
//
LOCUS       DAJTQY010000170         5863 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.170, whole genome shotgun sequence.
ACCESSION   DAJTQY010000170 DAJTQY010000000
VERSION     DAJTQY010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5863
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.170"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            <1..378
                     /locus_tag="OO349_003804"
     CDS             <1..378
                     /locus_tag="OO349_003804"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5868710.1"
                     /translation="IIRWLLKKRTNGSVRYCQYTSKVAMIYIEQLELIHKSGDVLYPV
                     KITRKSSGKTAFHLVPFGLNKTHDLLEVEDASEAIRLVIDERHSIRCSTLTATITNKK
                     GKRIKRTGIYSIKGVNIKEYNVR"
     gene            complement(454..918)
                     /locus_tag="OO349_003805"
     CDS             complement(454..918)
                     /locus_tag="OO349_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HCR5868711.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSAIENEQTIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(915..1790)
                     /gene="nanK"
                     /locus_tag="OO349_003806"
     CDS             complement(915..1790)
                     /gene="nanK"
                     /locus_tag="OO349_003806"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HCR5868712.1"
                     /translation="MTILAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVEMYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(1787..2476)
                     /locus_tag="OO349_003807"
     CDS             complement(1787..2476)
                     /locus_tag="OO349_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HCR5868713.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(2524..4014)
                     /gene="nanT"
                     /locus_tag="OO349_003808"
     CDS             complement(2524..4014)
                     /gene="nanT"
                     /locus_tag="OO349_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HCR5868714.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(4124..5017)
                     /gene="nanA"
                     /locus_tag="OO349_003809"
     CDS             complement(4124..5017)
                     /gene="nanA"
                     /locus_tag="OO349_003809"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HCR5868715.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(5139..>5863)
                     /gene="nanR"
                     /locus_tag="OO349_003810"
     CDS             complement(5139..>5863)
                     /gene="nanR"
                     /locus_tag="OO349_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HCR5868716.1"
                     /translation="SRPLARKKLSEMVEEELEQMIRRREFGEGEQLPSERELMAFFNV
                     GRPSVREALAALKRKGLVQINNGERARVSRPSADTIIGELSGMAKDFLSHPGGIAHFE
                     QLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQSLDNNAAFIRSDVDFHRVLAEIP
                     GNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVSYQQHIAIVDAIRRHDPDEADRAL
                     QSHLNSVSATWHAFGQTTNKKK"
BASE COUNT         1450 a         1625 c         1490 g         1298 t
ORIGIN      
        1 atcatcagat ggctgcttaa aaaaagaaca aatggtagtg tccgctattg ccagtacacc
       61 tcaaaagttg ccatgattta cattgaacag cttgaattaa tccataaaag tggtgatgtg
      121 ttgtatccag taaaaataac cagaaaatca tcaggaaaaa ctgccttcca ccttgttcca
      181 ttcggcttaa ataagactca cgaccttcta gaggtggaag atgcttcaga agcaattcgg
      241 ctggtgattg atgaacgtca ttccattcgc tgcagtacac ttacagcaac tattacgaat
      301 aaaaagggca agcgaattaa gcggacaggt atatacagta tcaagggtgt taacataaaa
      361 gaatacaacg tcaggtaatg gcacagagcg gtccgtcagt tcaggtttgg ctctgtgcca
      421 aagatgggtc aggtcacacc tgatctaata aatttaagcc attaaatcag ccttaacctt
      481 caccaccacc tttttaatct cacccggctc gccaacaacg catcccggct tatgcggctc
      541 tcctggcata aacacggcga acattcccgg tttcaggatg atggtttgct cgttttcaat
      601 ggcgctacaa agctggtaat catcttcatg gtggaactct tcacattggc gcgcagtgcc
      661 tgccatgcca aacagaatcc gttcctcacc gtttaataac agctggatat caatgtattg
      721 ctcgtgcaat tccgcttttt tctcgacggg cgattgagtg ttaaacgtca tgacattcat
      781 aaaaatattg tcgccctgta attcgtaacg acccggcgct ttttcttgcg gtctggcagc
      841 taaggccagc gttaacgcgt cctgtaacgc aggatgtaac ccagcagacg gtaatgactg
      901 tacttcaccc atcatcataa tttttctccc tgggccaaca gcgcagcccc aagtaaacct
      961 gcatcatggc ggtaatgcgc cgccagtaaa tcaacatgaa atgccgctgg ctcctgcgcc
     1021 agatacattt ccaccaacgc cagataccct tctgccagac caacgctgcc accaaccacc
     1081 acgcactggc aatcagttgt ggctttaata tcagcgatca gccttgcaag cgtacgtgcg
     1141 gagcgatgaa tcagttgctg cgcctgctca tcgccctgcc cggcgcgtgt gaaaatagtt
     1201 ctcgcatccg cgccagccag ctcgccctgc gctgccgccg caatgccgcg accagaagca
     1261 attgcttcca cgcaacctgt gcgtccacag ccgcagactg ggccgtgtgg atcggcaagc
     1321 gtatgcccga tgtgccccgc cagaccgcca gggccggtaa gcagtttgcc gccgctcact
     1381 acaccgccgc cgacgccagt ggaaacggtg ataaagacca tatcggttat atcgccttcc
     1441 agcgcctgat actctgccca tgctgcggcc tgcgcgtcgt taatggcaat ggtcggcaaa
     1501 ttggtaagtt gttccagcgt tttgactaac ggaaagtgta gcaatccacc gagattatgc
     1561 ggattcagcg ccagcaaact gccgtcgcga ataattccgg ttgaagcgat ggcaacccgc
     1621 tgcgcatgag cttgcaacgg agagactaat gcggataagg catcacgcaa ggcttctggt
     1681 gtctggctgg ctggcgtagg aagttcacga cgatcgcgga tctgcccgtc agcgccaatc
     1741 agcgcggcgg caagtttagt accgccgata tcaatcgcca gtatggtcat agcaccgcct
     1801 ttttcatcgc tgtgttgtac cactgacaaa tgtgttcaag acgcgtaatg gcagaaccga
     1861 ccgtcaccgc ccacgcgccg tggcgcatcg catctgccgc ctgagcaggc gtgttgtaac
     1921 gcccttcggc aatcacccga catccggctt cgctcaacgt tttcaccagc gccagatccg
     1981 gctcttctgg cgtttcaggc gtggtatagc cagaaagcgt agtgccaata atttcggctc
     2041 ccagcttttg gcatgccagg ccgtcttccg gcgttgagca gtcggtcatc gccagtaaac
     2101 cgtgatgatg gatacgtgcc agcagcgttt caacaggcac cggacgcggg cggtcggtgc
     2161 cgtcaatggc gataatgtcc gcgcccgcct gcgccagcgc atcgacatct tcaatatagg
     2221 ccgtgatgcg taccggagaa tcctccagat cgcgtttcac aattccaata atcggcacgc
     2281 tcaccaccgc acgcgtggct tgcagatttg ccacaccttc gatgcgaatg gcaaccgcgc
     2341 ccgcctgctc tgccgctaat gccatggctg cgacgatttc gggtttatcg agcgggctgt
     2401 caggaaccgg ctggcaggag acaatcaggc caccgttagc agcgattttt tgatccagtt
     2461 gtgcaagtaa cgacatacat cttcccttag cgaaaggccc ggtacataga ccgggcaaca
     2521 ggattaactt ttggttttga ctaaatcgtt tttggcgctg ccaaatggca cggcaccgct
     2581 aaatggtttg ccgtcgatag cgtcatgagt acgcaatgct tccgggcgca gccaacgctg
     2641 aacgcgggaa ggcatatcca gaccaatcag caggatcacc acaaatgtca ggctgaacga
     2701 gagcgatgcc agcgcagtac ccagatccag acgttgagcg atcaacgcgc cgatgattgg
     2761 ggccagtgcg ccacccaatg cgccaacgtt gtaggtaaag cccaggcccg ctgcacgctg
     2821 gtcggtatcg aaataaccgc caatcagttt cggtaagatc ccggcgatcc cttgtccaag
     2881 catttgctgg aagaacagta acagaccgag cacccagacg tttgcgccgc caatcgcaaa
     2941 taccggaata atcagcagct gcgaggccag caggctacaa acgtacgctt tgcgggttcc
     3001 cagccagtca ccgaggaagc cacctacgca gcatcccacg gccgcgccaa agccactaaa
     3061 gaacagcaca ttggcgacag tatgcgggtt ataagccaga tcggttttca gatacgttgg
     3121 cagcagcgcc tgaatcggcc atgagtagag gaaagcaaac aacacgacca ccatcagcat
     3181 tacgcccgtt ggccagcgtt tacccgtact ttgcaccata aagctgataa agattgcggc
     3241 gcataacagc ccgagaacag cgacgattgc agcattttgc aggttaccgg cgaagcagaa
     3301 ccacagcgca gtagccgccg ccagtgtcat tacgatattg gcaatgcgat gttcaccacg
     3361 gtagagaata tccaccattg tgcgtactgg tgctttacct gcgtgtttct ctttccagtc
     3421 ttccgcttcc gggatgtttt tacgcagcca gagagcaaag atgattggca aaatgccgat
     3481 aaagaacagc gcacgccaac cccagaccgg aaccaccagg ctatagacct gagcggcaac
     3541 gacggccccc acagagaagc ctgaaatcaa aaaaccactg gctttgttac gcagatgttt
     3601 tggccagctt tcaatgacat aggtggcgct ggaaccgtat tcacccgcca tccccatgcc
     3661 gatgaccaga cgagcgataa acatggtgat gtagcctggc gcaaagccgc aggccagcgt
     3721 cccggccgag aagagaacga tgctggtgac cattgccaga cgacgcccgt agcggtcacc
     3781 catagcgccg agcatcaggc cgccgaacca gcgagagata aaggctgcgg agatcagact
     3841 tgccgcctgc accgtcgtca gcccgaattc accctgtact tcggtgagta ccagggcgat
     3901 taaaacgaaa tcaaaaccgt caagcagata tcccaaccag gcagcggaaa atgcgcgcca
     3961 ttgtgcacgg ttgagatggc gataccacgg gatattctgg gttgtagtac tcattgtgag
     4021 tctcccgcgg tgggcgatgc ccacacgctt tggtatgaaa attgtagggt acagatgcgt
     4081 ttatttcccc tcacccggta ggggcgagcg aggggaaaac aactcacccg cgctcttgca
     4141 tcaactgctg ggccagcgcc ttcagttctg gcagatattt ttcatctacc ggtccaaacg
     4201 gtttgcggca cagcggcaca gaaacgacat ccatataatg caggacagtt ttcaggccgc
     4261 ggaatacgcc cgttttgatc agtaaatcaa tgactttgtt gcactcagtt tgcagtttct
     4321 gcgcggtctg gatatcgcct tctttcagcg ccttcacgat cccctgatat cgccagccca
     4381 tgatgttgta agtactgccg ataccaccat cagcgcctgc cagcaagccg gaagcgaaga
     4441 tttcgtcgta accgttatag agcaccagat caggatgttc acgacggatc tgttccatct
     4501 gatagagatc gccagaagtc tgtttcagcg caccgacgcc aggcaacgta accagggtgt
     4561 tgatctgatc cagggtcagt tttaccccac tcagggctgg aatgttgtac accaccatcg
     4621 gcaaaccatc cgctgaatca ataattgccc gatagtgatc gcagtgttct tcaaagctga
     4681 aaggatagta gaacggcgtg acggcggaga cggcatcgaa gccataacgt ttagccgatg
     4741 ccgcaagttg ttggctttcg gcggtgctga cgcaaccgac gtgggcgatg agtttaatct
     4801 tacctttcgc ctcttcggcg acgatttcca gtacctgttc acgctcggaa aggctttgta
     4861 caaaggcttc gccggtcgaa ccacccacgt ataaaccgtc gatgccctgc tgaatattga
     4921 actgaaccag gcggcgcaga ctcgctttat ccagtgcttg ttgttgatca aaaggagtca
     4981 ggagtgcagc cattacgcca cgtaaattcg ttgccataaa tacctctgaa gtgatgcttg
     5041 tctgataaac gatatacctt tatacctgtt ataccagatc aattaagcaa caccccatac
     5101 agaaagctta taatgcgatc tgcttcacta aagtggcatt atttcttttt gttggtggtc
     5161 tgaccgaaag cgtgccaggt agcagagacg ctgttgagat gcgattgcaa cgcacgatcg
     5221 gcttcgtcag gatcatgacg gcggatcgca tcaacgatcg caatatgctg ttgataacta
     5281 acgttgttat gttcgtgcag tgcctgatcg gtaaccgttg ggcgtgcggc aataagccag
     5341 tcgagcaggg caacgtggat cgccatgaag attgggttac cggggatctc cgccagcacg
     5401 cggtggaaat caacgtctga acgaatgaat gccgcgttgt tatccagcga ctgactgttg
     5461 atttccagtg cttttgccag caaatcgatt tgctcatcgg tggcgtgttc agccgcatag
     5521 cgcaccaggc tggattcaaa gaacagacgt aattgttcga aatgggcaat cccaccagga
     5581 tgagaaagga aatctttcgc catgccggaa agctcgccga tgatagtgtc cgcagaaggt
     5641 cgagagacac gagcgcgttc gccgttgttt atttgcacca gacctttgcg ttttaacgct
     5701 gccagcgctt cacgcacaga aggacgcccg acgttaaaga acgccatcag ttcgcgttca
     5761 gacggtaatt gttcaccttc gccaaattca cgacggcgga tcatctgttc cagctcttct
     5821 tccaccattt cggagagttt tttacgcgcc agcgggcggc tac
//
LOCUS       DAJTQY010000171         5758 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.171, whole genome shotgun sequence.
ACCESSION   DAJTQY010000171 DAJTQY010000000
VERSION     DAJTQY010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5758
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.171"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(<1..402)
                     /locus_tag="OO349_003811"
     CDS             complement(<1..402)
                     /locus_tag="OO349_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tar ligand binding domain-containing protein"
                     /protein_id="HCR5868717.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNFDEKYKNYYTAL"
     gene            complement(547..1050)
                     /gene="cheW"
                     /locus_tag="OO349_003812"
     CDS             complement(547..1050)
                     /gene="cheW"
                     /locus_tag="OO349_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HCR5868718.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(1071..3035)
                     /gene="cheA"
                     /locus_tag="OO349_003813"
     CDS             complement(1071..3035)
                     /gene="cheA"
                     /locus_tag="OO349_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HCR5868719.1"
                     /translation="MSIDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASYDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQLPR
                     RIILSRLKAGEVDLLEEELGHLTTLNDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKIATPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(3040..3966)
                     /gene="motB"
                     /locus_tag="OO349_003814"
     CDS             complement(3040..3966)
                     /gene="motB"
                     /locus_tag="OO349_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HCR5868720.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRLHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(3963..4850)
                     /gene="motA"
                     /locus_tag="OO349_003815"
     CDS             complement(3963..4850)
                     /gene="motA"
                     /locus_tag="OO349_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HCR5868721.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGGVHALGSADRPAAELGVLIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(4977..5555)
                     /gene="flhC"
                     /locus_tag="OO349_003816"
     CDS             complement(4977..5555)
                     /gene="flhC"
                     /locus_tag="OO349_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HCR5868722.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRARTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(5558..>5758)
                     /locus_tag="OO349_003817"
     CDS             complement(5558..>5758)
                     /locus_tag="OO349_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HCR5868723.1"
                     /translation="LPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGI
                     MLSTRLLNDVNQPEEALRKKRA"
BASE COUNT         1341 a         1553 c         1407 g         1457 t
ORIGIN      
        1 taacgctgtg taatagtttt tatatttttc atcaaaatta cggctggtag cgaccatttc
       61 aggtaacggt gccatgcttt tgaatttttt ataatgcgtc gcggcctggg ccaatgtttt
      121 cctagcgcta tcgagcaatt caactttggc gttactttgt tgattggagg aatccatcat
      181 catccgcacc gctgaacgac tcaggttaat gcgcgtttgc agcattaaat cccaggttga
      241 cgtcagctcg ccctgctgtt cccgtaattg attggaaacc acaaagctct tctggctatg
      301 gtgaagggaa gaaaaaaaca aactgccgga aataagctgt aacagtgcga atacccccag
      361 caccattacc aacagcgtga ctacgcggat acggttaatc ataaggcacc ttcctgaaaa
      421 caagttgatc tcgttatcgg caagaagggg ggaaacttta ttgctgatgc cacccgccgc
      481 gaaattgaaa taaaaaaccc gatgcgcaga tcatcgggtt catttcaatt gaggaaatcg
      541 ggagaattac gccacttctg acgccgcgct atctaacagc gccatctctt cgctgttcag
      601 cagtttttcg atgttcacca gaatcaacat acggtcgccc agtgcgccca gtccagtgag
      661 atattctgtt gaaagcgtca cggcaaactc tggtgccgga cgaatttgct ccgccgtcaa
      721 tgaaagcacg tctgaaacgc cgtcaaccac gatgccgacc acccgctgtc cgagattcag
      781 gacgataact accgtgttgt cgttatagtc cacatccacc tggctgaact taattcgtaa
      841 gtcaacaatc ggcacaataa cgccgcgcag attcgtgacg cctttgataa acgctggcgt
      901 gttcgcaatc cgtgttacct gatcgtagcc acggatctcc tgcactttca ggatatcaat
      961 accgtactct tcatcaccaa gggtaaatac cagaaattcc tggcctgacg gctcgctggc
     1021 cagctttgtt acattcgtca taccggtcat attgttacct ttttactcat tcaggcggcg
     1081 gtgttcgcca tacgttgttc gcggtttatc gcctgcaagg cggagacatc aacaatcagt
     1141 gccacgctgc cgtcgccaag aatggtcgca gcagaaatgc cggggacttt gcgatagtta
     1201 ctttcaaggt ttttaaccac aacctggtgt tgaccaatta attgatccac cagcaaggcg
     1261 tagcggcgac cgccactttg taagatcacc acaattccct gggtggcttc ggttttcgcg
     1321 cccgcgacgt tgaacacttt ccacagttcg acgatgggca gatattcacc ccgcacttcc
     1381 agcacccgct cgccgccggc cagtggatgg agatcggctt cacggggttg cagtgattcc
     1441 ataacagcat tcagcggcag aatgaaaact tcatccgcaa cgcgtacgga catgccgtcg
     1501 aggatggcca gcgtcagcgg cagtaaaatg cggatcgtag tgccagtacc ctgcttcgac
     1561 tggatttcga catgaccgcc catctcctgg atattacgtt taacgacgtc catgccgacg
     1621 ccgcgcccgg agacgtcggt gacctgctct gccgtggaga agcctggtgc aaatatcagc
     1681 attgcaactt cgtcgtcgct catgttttcg ctgacagtca aaccttgcga ggccgctttt
     1741 gccagaattc gctcacggtt tagccccgcc ccatcgtcgg tcacttcaat gcaaatgttg
     1801 ccgccctgat gttcggcaga cagaattaaa tttccgacgc tgtttttacc tgcggcgagc
     1861 cgtttttctg gcagttcaat accgtgatcg aggctattgc gtaccaggtg ggtcagcggg
     1921 tcgataatgc gttctatcag gctcttgtcg agctcggtgg aactgcccac cagcgtcagt
     1981 tctacctgct tgccgagttt tcccgccaga tcacgcacca gccggggata gcgactaaag
     2041 acatattcca tcggcatcat gcgaatcgac atcactgatt cctgcaaatc acgggcgtta
     2101 cgttgtaact gccccatgct ggtaatcaaa tcaccatgat taaccgggtc cagttcgctg
     2161 gaacgctggg caagcatgga ctgggtgata accagctcgc cgacgaggtt aattaattga
     2221 tcaacctttt ctaccgctac acggatgctg gtggattcac tgctgcgcgt cgttttttcc
     2281 cgctccacgc ggccggttgg cgcttgttcg gctgccagtt taagcacagg tggggtagct
     2341 atttttggcg aaacttctac tgtttcaaag gtaatctgat cggcttcaat cacaaaacag
     2401 agtaccgctg tgatgtcatc ttcggcgata tcgcccggta atattgccga gagagaatcc
     2461 gcccctttca ccacgtcatt taacgttgtc agatgcccca gctcttcttc cagcaggtcg
     2521 acttcgccgg ccttcaggcg cgaaaggata attcgtcgcg gcaactgact gcgactttgc
     2581 tcatcttgcg gttcactttt ggcaaccaca cttaatcggg tcactgcgga tggcgtttcg
     2641 cctttcgctt ctaatgccag ttgacgcaag gcctggcaga tataatcgta gctggcggca
     2701 tccggctctt gcgactgttt ataagcgtcg agctgttctt gcatgatgtc cttcgtttcc
     2761 aaaaacagat tgataatgtc ggtgttgagt tgcatctcac ctcgtctggc ttcatcgagc
     2821 aggttttcca tcagatgcgt ggtttcctgc aaaacgctga agccaaaagt tcctgcccct
     2881 cctttgatcg agtgggcagc ccgaaagatg gcattcaatt gttcggcatc tggcgcttcc
     2941 ggctgcaaaa ccagcagatg ctgctccata tcagccaaca gttcgtccgc ttcatcaaaa
     3001 aatgtctgat aaaaatcgct tatatcgatg ctcacgctgt cacctcggtt cggctgatgg
     3061 cattgtggga acactgacct gtggtgcaac ctcaggtttt tctagggcgc ttactggctc
     3121 attctggctt tcggcgtttt catgcaaaat ggcctgttcg gcttgtttgt tcagtaccaa
     3181 caggctgatg cgacggttga cggcatcatc aggtccgcga tcgcttaagc gcatcgttgc
     3241 cgccatgccg acgacacgta acactttgcc gctatccaac cctccgacca tcagttcgcg
     3301 gcgggatgca ttggcccgat cggcagaaag ctcccagttg ctgtaacctt tctcaccgct
     3361 ggcgtaggga aaatcatcgg tatgacctga aaggctaata cggttgggaa taccgttcag
     3421 cacaggcgcg atggcgcgca gaatgtcgcg catatagggt tcgacatcgg cactgccggt
     3481 tctaaacatc gggcgattct ggctatcgat gatctgaata cgtagacctt cctggaccag
     3541 atcgattttg agatggagac gtaacgcccg cagtttcgga tcggactcta tcaactggtc
     3601 gagatcaccc cgcaatttcc gcaatcgact ttgctccatg cgttttttca gctcttcgat
     3661 gttcggctgc ttattcactt ccccctggct ctgggtgtaa tcatcaccac cgccgggaat
     3721 tgggctttca ctattggaaa tgcgatcgcc gcccgtaacc gctgtcgcca gtggagtccg
     3781 gaagtactcc gcaatctgaa tcaactcttt tgggctggag atggagatca gccacatcac
     3841 caaaaaaaag gccatcatcg cagtcataaa gtcggcataa gcaatcttcc acgatccatg
     3901 tgctgccccg tggcttttgg ctttgcgtcg tttgacgaca ataatcggat gcgcttgatt
     3961 cttcatgctt cctcggttgt cgtctgttgt tgcggatttt tcaccgcacg cacatgctct
     4021 tccagttcaa taaacgacgg acgttcgctg gaatagagcg ttttgcgacc aaactcaacg
     4081 gcgataggcg gtgcgtaacc gttcagatta gaaagcagag tgactttgac gcactgcatc
     4141 attttgctgg tttcggcgct tttctgacgt aaaacactcg ctagtgggga aataaatccg
     4201 taagccaata aaataccgag gaaagtcccc accatcgcat gtgcgataag cacccccagc
     4261 tcggcggcag gacgatcggc tgaacctaac gcgtgaacgc cccccattac agccgcgaca
     4321 ataccaaacg ccggaagtga gtccccgacc agcgccagac tgttcgccgg gacttctgcc
     4381 tcgctttcgt gcgtctcaat ctcttcatcc atcagggctt cgatttcgaa ggtgttcatg
     4441 tgaccgctga taatcaggcg cagataatcg acgataaaat caagcatgac gctatccgcg
     4501 aggatgcgtg ggtagctggc gaagatctcg ctctcacggg gattttcaat atcacgttcc
     4561 agcgaaaaca tccccatctg ccgcgatttc gccatcaacc gataaagcag agccagcaga
     4621 tccatataca ttgctttggt gtatttggag cgacgaaaca gcaacggcag cgctttcagc
     4681 gtgcctttaa tcgctttgcc attattgccg acgataaacg acccaatccc tgcaccggca
     4741 ataatcacca gttcagcggg ttgatagagt gctccaaggc ttccaccggt catcaaataa
     4801 ccgccgaaaa ctgtaccgag aacaaccagg taacctaata agataagcac gacatcatcc
     4861 ttccactgtt gaccatgaca ggatgttcag tcgtcaggcg ttaacgcgcg attggggaaa
     4921 aaaaaagcag cggtacgtcg ttaccgctgc tggaatgttg cgccacaccg tatcagttaa
     4981 acagcctgta ctctctgttc atccagcagt tgtgggataa tatcggcagg attctgggaa
     5041 agtttacgtc tttttactgc ccgggatggc ggttgacata agctgcaggc aaagctgcca
     5101 acaggctggt gagcgtgggt aataaaattg ccgccgcagc agttgcagct ggaaagttgc
     5161 agtaatccac tttcaacaaa ccgcaccaat gtcctggcac gggttaatgc cagcagtggt
     5221 ccttcttctg cttgtgggca ctgttcaagg tataaacggt aggctttgat caccgcatcg
     5281 acgccattac acaaaccggt tttcagtaaa aactgccatg cattacagaa catcgaagca
     5341 tgaacgtttt gttcccaggt cataaaccag tcggttgaga atggcagcat gcctttcggc
     5401 ggtgggcttc cgcgcagttc tttataaagt tttatcaggc gtccgcgact taactgtgtt
     5461 tcgctttcca gcatctgcaa acgagcgccc agggtgatca attccattgc cagctgaata
     5521 tcccgcgctt cctgaacaat gcttttttca ctcatgatca ggcccttttc ttgcgcagcg
     5581 cttcttcagg ctgattaaca tcattcagca agcgtgttga gagcatgatg ccggtatgaa
     5641 tttgctggag atcgtcaacg cgggaatctt gcgtcaactg agtaatcgtc tggtggctgt
     5701 caaaacggaa gtgacaaacc agctgattgg tttctgccag cttaaccatt tgcggaag
//
LOCUS       DAJTQY010000172         5702 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.172, whole genome shotgun sequence.
ACCESSION   DAJTQY010000172 DAJTQY010000000
VERSION     DAJTQY010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5702
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.172"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(<1..219)
                     /locus_tag="OO349_003818"
     CDS             complement(<1..219)
                     /locus_tag="OO349_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HCR5868724.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQ"
     gene            complement(216..1124)
                     /locus_tag="OO349_003819"
     CDS             complement(216..1124)
                     /locus_tag="OO349_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCR5868725.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAVFSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            1320..2087
                     /locus_tag="OO349_003820"
                     /pseudo
     CDS             1320..2087
                     /locus_tag="OO349_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
     gene            complement(2138..2790)
                     /gene="pphB"
                     /locus_tag="OO349_003821"
                     /pseudo
     CDS             complement(2138..2790)
                     /gene="pphB"
                     /locus_tag="OO349_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
     gene            complement(2896..5457)
                     /gene="mutS"
                     /locus_tag="OO349_003822"
     CDS             complement(2896..5457)
                     /gene="mutS"
                     /locus_tag="OO349_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCR5868726.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRSNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            5533..>5702
                     /locus_tag="OO349_003823"
     CDS             5533..>5702
                     /locus_tag="OO349_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5868727.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIVSIG
                     EPFFFRVIVPFK"
BASE COUNT         1339 a         1480 c         1470 g         1413 t
ORIGIN      
        1 ctgttgcaac cagctcagag ccgccagcga ttgctgtgtg gcttcaacca ccggacagga
       61 gcgcgttttc aggctgatca ccagtgcgtc gattgcttcc ggcattttac ctgttggaac
      121 accgttaatt tgtaccgttg gtagaccgtt ttccaccaga aaactggcga tatccgtcgc
      181 gccggtaaaa tcatcggcga taacgccaat cttgatcatg atttcgctcc cggtagagtg
      241 atgccagaga aaatcttgat aactgcgcta tcgtcttctt tcccgtaacc cgcgttactg
      301 gcgctggtga acatattcaa tgctgttgag gccaatggca gcgggaagtg cagggctttg
      361 gctgtatcgg caaccagacc aagatcctta acaaaaatat cgacggctga atgcggggtg
      421 taatcgccat ccaccacatg acgcatccgg ttttcgaaca tccaggaatt tccggcggca
      481 ttggtcacga cgtcatacat cacatccagc gggatccccg cacgggctgc aagtgccatc
      541 gcttcggctc cggcagcaat atgtacgccc gctaacaact ggtgaataat ttttacggtc
      601 gaacctagtc ccggttctgc acctatgcga taaacttttc cggcaacggc ttccagcacg
      661 ggtgccagtc gttcaaaggc aatatcgcta ccggaggcca tgacagtcat ttcaccgtta
      721 gcggctttta ctgcaccacc agaaactggc gcatccagca tttccagatc gaatccagcc
      781 agagcggtag caatttcttg cgcatcagca ctagcgatag tggaagaaac cattactgcc
      841 gtaccgggtt tcagatgttg tgcaacgcct gtttcaccaa acagcacctg tttaacctgg
      901 gccgcattga ccaccagcac cagcagagcg tccagttttt cggcaaacgt cgcggcgtta
      961 tcagaaaccc cgcaagcacc tgcctctttc aacgtagcgc aggcattgct gttcaggtct
     1021 gcgccccagg tagaaaaaac tgcgcggaca catgacagtg ctgctcccat tcccattgac
     1081 cctaagccaa cgataccgac atgaaactca gatcccgttt tcatctgctc tccttgttaa
     1141 tttaagtgat attttgtttg atattgtgaa cataagcgct gggagataac gatatggtga
     1201 gatgattcac ataaattaac attttgtgtt attttatgtg aactaagcgt tagttgcgcc
     1261 gcgcacgttt cgcaggcaaa tagcgtagaa tgtcagcagg acaaaggaag gagcaaaagt
     1321 tgatacccgt agagcgtcgc caaatcatcc ttgagatggt agctgaaaaa ggcattgtca
     1381 gtattgctga actaacggac agaattaatg tgtcacatat gaccattcgt cgggatttac
     1441 aaaaactgga gcagcaggga gccgttgtgc tggtgtccgg aggcgtctag tctccgggac
     1501 gcgtggcgca tgaaccttct catcaggtaa aaactgcgct ggcaatgacg caaaaagcgg
     1561 ctattggcaa gctggcggca agtcttgttc tgccgggaag ttgtatctat ctggatgcgg
     1621 gaacgaccac gttagcgata gcacagcatc tgattcacat ggagtcactg actgtggtca
     1681 caaacgattt cgttattgcg aactacttgc tcgacaacag taattgcaca attattcaca
     1741 ctggcggtgc agtgtgtcgg gaaaaccgtt cctgtgtcgg ggaagccgct gcgaccatgc
     1801 tgtgcagcct gatgattgat caggctttta tttctgcatc gtcatggagt gtgcggggga
     1861 tttctacgcc agcggaagat aaagtcacgg tgaaacgggc gattgccagt gccagccgcc
     1921 agcgagtttt ggtctgtgat gcgacgaaat atggtcaggt ggcgacatgg ctggcgttac
     1981 cattaagcga gtttgatcag attattacag acgacggttt gccggagagt gccagtcgcg
     2041 cgctggcgaa gcaggatctc tctttgctgg tagcgaaaaa tgaataatgg cctgcaataa
     2101 catctggtta ctcatgcttc acagaagaag catgagacta ccttatttta taaaatgaca
     2161 gccgcccact tttcggcgat ccggtatcaa tataaatctg gttagcgaac gtctgaatgt
     2221 tatcaaacat catatgtcca aatataaaat aatcagcgcc gtttatttgt tgtaactcgc
     2281 cattaagcga tttctgcaca cgataaacag gccagagtaa ttcgctctcc gctatttctt
     2341 tgccaaagag atattcatca cccggataat ctgcatgtgc gatgacatat tttattatgt
     2401 cgttagtgat ttcaataata tgtggaaggt gatggaattt cagcaacaga tctattgcct
     2461 cttgttgcaa tcatttaaat cgaaaaacca gtcaccaccg ctggcaagcc acatattgcc
     2521 atcgccggtc gcgaacgcat ccagcgccat tgcttcgtgg ttgcctttaa cggagataaa
     2581 ccagggctgg tttagcaggc gcaggacgtc aagactctcc ggcccacgat caatattatc
     2641 gccgacagaa ataagtaagt cggtttcggg gaaaaaagag agttgatgta agcgggattg
     2701 taataactga tattcaccat gaatatcacc aacgacccat atatggcgat agtgatgggc
     2761 attgattttt tggtagcgtg tagatggcat ggttttaccc tgtaaaataa gctttcctat
     2821 tatacagggt atttttattt gattcgtcag ttgtcgttaa tattcccgat agcaaaagac
     2881 tatcgggaat tgttattaca ccagactctt caagcgataa atccactcca gcgcctgacg
     2941 cggagtcagt gaatccgggt cgagattttc cagtgcttcg accgcaggcg aagtttcttc
     3001 tggcactgac agcaaagaca tctgcgtacc atccacttgc gtcgcggcgg cgttcggcga
     3061 aatgctttcc agctcacgca gtttttgccg tgcgcgctta ataacctctt tcggtacgcc
     3121 cgccagagca gcaaccgcca gaccgtagct tttgctcgcc gcgccgtcct gcacgctgtg
     3181 cataaaggcg atagtgtcgc cgtgctccag cgcatcgaga tgtacgttgg cgacgccttc
     3241 cattttctcc ggtagctggg tcagctcgaa atagtgggtg gcaaacagcg tcaatgcctt
     3301 aatcttattc gccagatttt ctgcgcacgc ccacgccagc gacagaccat cgtaggtgga
     3361 cgttccacgc ccgatctcat ccattaacac cagactgtat tcggtggcgt tatgtaaaat
     3421 attggcggtt tcagtcatct ccaccataaa ggttgagcgc ccggaagcca ggtcatctgc
     3481 cgcgcctacg cgggtaaaga tgcgatcgat aggtccaatc tcgacttttt gtgccggtac
     3541 atagctgccg atgtaggcca tcagcgcaat cagtgcggtc tggcgcatat aggtactttt
     3601 accgcccatg ttcgggccgg taataatcaa catacggcgc tgcggcgaca gattcagcgg
     3661 gttagcgata aatggctcat tcagtacttg ttcaactacc ggatggcgac cttcggtaat
     3721 gcgaatgccc ggtttatcaa tgaaggtcgg gcaggtgtag ttcagggtat aggcccgttc
     3781 cgccaggtta accagcacgt cgagttccgc cagcgcgctc gcgctctgtt gcaacgcttc
     3841 cagatgcggc aacagcaggt cgaacagctc ttcataaagc tgtttttcca gtgccagtgc
     3901 tttgcctttt gaggtgagaa ctttatcttc gtactctttt agctctggaa tgatgtagcg
     3961 ctcggcgttt ttcagcgtct ggcgacgcat gtagttgatg ggtgccagat ggctttgccc
     4021 acggctgatt tgaatgtagt agccgtgcac cgcattaaag ccaactttta gcgtgtccag
     4081 gccggtacgt tcacgctcgc ggacttccag acgctccaga taatcggtcg cgccgtcagc
     4141 cagcgcgcgc cactcatcca gctcttcgtt atagcccgat gcgataacac caccgtcgcg
     4201 taccagcacc ggcggtgtgt cgatgattgc tcgctccagc agatcgcgca gctcggcaaa
     4261 ctcgcccatc ttctcacgta gcgcctgtac cggtgcacta tcgacagttt ctaactgcgc
     4321 acgcagctcc ggcagttgct ggaaagcgtg gcgcatacgg gccagatcgc gtgggcgagc
     4381 agttcgtaaa gccagacgtg ccagaatacg ttccaggtcg ccgacctgac gcagtaccgg
     4441 ctgcaactcg gcggtgaaat cctgcaatgc gccaatagtt tgctggcgct caagcaacac
     4501 gcgggtatcg cgcactggca tatgcagcca gcgtttcagc atacgactac ccatcggcgt
     4561 gacggtgcag tcgagcacag aagccagcgt attttccgca ccgcccgcca ggttctgagt
     4621 aatttccagg ttacgacgtg tcgcggcatc cataatgatg ctgtcctgct cacgttccat
     4681 ggttatagaa cgaatatgcg gcagggtcgt gcgttgggta tctttcgcat actgcaacag
     4741 acaaccggca gcacaaagtc cgcgcggcgc gttctcgacg ccaaaaccga ccagatcgcg
     4801 ggtgccaaat tgcagattca actgctggcg cgcggtgtcg atttcaaact cccacagcgg
     4861 gcgacggcgc aggccgcgac ggccttcaat cagcgacatc tcggcgaaat cttctgcata
     4921 cagcagttcc gccggattag agcgttgcag ctctgccgcc atcgtttcgc ggtcggccgg
     4981 ttcgctcagg cgaaaacgcc cggagctgat atccagcgtc gcgtagccga aacctttgct
     5041 gtcctgccag atagccgcca gcaggttgtc ctgacgctcc tgtaacaggg cttcatcgct
     5101 gatggtgcct ggcgtaacga tacgcacaac tttgcgctca accggacctt tgctggtcgc
     5161 cggatcgcca atttgttcgc agatggcaac ggactctccc tgattcacca gtttggcgag
     5221 atagttttcc accgcatggt agggaatccc cgccatcggg atcggctctc ccgccgaagc
     5281 accgcgtttg gtcagtgaaa tatccagcag ttgcgacgcg cgttttgcgt cgtcataaaa
     5341 cagttcataa aaatcaccca tccggtaaaa cagcaggatc tcgggatgct gggctttcag
     5401 cttgagatac tgctgcatca tgggcgtatg ggcgtcgaaa ttttctattg cactcatggg
     5461 gttatgtccg gttccctgat attaaatggg gtgtgatggt ttttattttc gttgtaatca
     5521 cataaggcgc acatgatacc ggagttaatc atttcagggg aagaagcgaa cggcgagttc
     5581 ttgatgctgg ttccgggaaa gatgctgttt tgcagctttt gcgtgatgct tatgaagcaa
     5641 gtacgcaaaa ttgtatcgat tggcgaaccc ttcttcttta gagtcatagt gccctttaag
     5701 at
//
LOCUS       DAJTQY010000173         5692 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.173, whole genome shotgun sequence.
ACCESSION   DAJTQY010000173 DAJTQY010000000
VERSION     DAJTQY010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5692
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.173"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(25..591)
                     /gene="satP"
                     /locus_tag="OO349_003824"
     CDS             complement(25..591)
                     /gene="satP"
                     /locus_tag="OO349_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HCR5868728.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(740..1453)
                     /gene="msyB"
                     /locus_tag="OO349_003825"
     CDS             complement(740..1453)
                     /gene="msyB"
                     /locus_tag="OO349_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HCR5868729.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSILM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSTYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            complement(1479..1883)
                     /locus_tag="OO349_003826"
     CDS             complement(1479..1883)
                     /locus_tag="OO349_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HCR5868730.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            2260..4176
                     /gene="dnaK"
                     /locus_tag="OO349_003827"
     CDS             2260..4176
                     /gene="dnaK"
                     /locus_tag="OO349_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HCR5868731.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTIPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            4265..5395
                     /gene="dnaJ"
                     /locus_tag="OO349_003828"
     CDS             4265..5395
                     /gene="dnaJ"
                     /locus_tag="OO349_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HCR5868732.1"
                     /translation="MAKQDYYEILGVSKTAEEREIRKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(5499..5651)
                     /gene="mokC"
                     /locus_tag="OO349_003829"
     CDS             complement(5499..5651)
                     /gene="mokC"
                     /locus_tag="OO349_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HCR5868733.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
BASE COUNT         1496 a         1412 c         1495 g         1289 t
ORIGIN      
        1 ccgagcgggg ggatctcaag acaattagtg ggattcacca atcggcagaa cggtgcgacc
       61 aaactgctcg ttcagcactt cacccatcgc cagatagatt gcgctggcac cgcagatcag
      121 accaatccag ccggcaaagt ggatgatcgc ggcattaccg gcaatgttac cgatcgccag
      181 cagggcaaac agcacggtca ggctaaagaa aacgaattgc aggacgcgcg cgcctttcag
      241 agtgccgaag aacataaaca gcgtaaatac gccccacaga cccaggtaga caccaaggaa
      301 ctgtgcattt ggcgcatcgg tcagacccag tttcggcatc agcagaatcg caaccagcgt
      361 cagccagaaa gaaccgtaag aggtgaatgc ggttaaaccg aaagtgttgc cttttttgta
      421 ctccagcaga ccagcaaaaa tttgcgcgat gccgccgtag aaaatgccca tggcaagaat
      481 aataccgtcc agagcgaaat aacccacgtt gtgcaggtta agcagaatgg tggtcatgcc
      541 gaagcccatc aggcccagcg gtgccggatt agccaactta gtgttgccca taattcctca
      601 aaaatcatca tcgaatgaat ggtgaaataa tttccctgaa taactgtagt gttttcaggg
      661 cgcggcataa taatcagcca gtggggcagt gtctacgatc ttttgagggg aaaatgaaaa
      721 ttttccccgg tttccggtat cagacctgag tggcgctaac catccggcgc aggcaggcga
      781 tttgcagtac ggctggaatc gtcacgcgat aggtgctgcc gctgaccgct ttaaccccat
      841 ttaatgctgc gcccacaggg cctcccagcc ccgcgccgcg cagcaaacca tgcccaagta
      901 cgctcattgc tgcgtgggtg cgtaaaatgc gggtcagttg gctggaaagc aaatgcgaca
      961 caccttttgc caataatttg tctttcatca gcagcggcag cagctcttcc agctcattca
     1021 ccctggcatc gaccgcgtgc agaaactcct gcttatgttc ctcgtccatt ttcttccagg
     1081 tattacgcag aaattgttcc agtaactgtt gctcaatttc aaacgtagac atctctttgt
     1141 cggctttcag cttcaatcgc tttgaaacat cgagcaaaat ggcccgatac aatttaccgt
     1201 gtccgcgcag tttgttggcg atactatcgc caccaaaatg ctgtaattct ccggcaatca
     1261 gctgccagtt gcggcgatgt tgctcgggat gcccttccat cgatttaaac agttcgttgc
     1321 gcatcagtat gctggagagg cgagttttgc ctttttcatt atgggtgagc aatcgggcga
     1381 aatttgccaa ctgttcctca ctacaatgct gaagaaaatc cagatctgaa tcattcaggt
     1441 aattaacatt cattttttgt ggcttctata ttctggcgtt agtcgtcgcc gataattttc
     1501 agcgtggcca tatccgatga gttcaccgta tgaccggaaa aggtgatttt tgagacgcag
     1561 cgtttattgt cgttatcgct gttaatgttg atccagtcag tggtttgccc ttcttttatt
     1621 tctgaaggaa tattcaggct ctgactggcg ctacgggcgg ctttgaaata aaccgatgca
     1681 ccacttaact gtaaatcgcc atggtcggca gagagttgta tgcgtttcac aatgcgacaa
     1741 acaggaagtt tcagcgccag atcgttggtt tcgttacgcg gcattgcaat gacgccgagg
     1801 agtttatggt cgtttgcctg cgccgtgcag cacagcatca ggctaatcgc caggctggcg
     1861 gaaatcgtaa aaacggattt cataaggatt ctcttagtgg gaagaggtag ggggatgaat
     1921 acccactagt ttactgctga taaagagaag attcaggcac gtaatctttt ctttttatta
     1981 caattttttg atgaacgcct tggctgcgat tcattcttta tatgaataaa attgctgtca
     2041 attttacgtc ttgtcctgcc atatcgcgaa atttctgcgc aaaagcacaa aaaatttttg
     2101 catctccccc ttgatgacgt gggttacgac cccatttagt agtcaaccgc agtgagtgag
     2161 tctgcaaaaa aatgaaattg ggcagttgaa accagacgtt tcgcccctat tacagactca
     2221 caaccacatg atgaccgaat atatagtgga gacgtttaga tgggtaaaat aattggtatc
     2281 gacctgggta ctaccaactc ttgtgtagcg attatggatg gcaccattcc tcgcgtgctg
     2341 gagaacgccg aaggcgatcg caccacgcct tctatcattg cctataccca ggatggtgaa
     2401 actctggttg gtcagccggc taaacgtcag gcagtgacga acccgcaaaa caccctgttt
     2461 gcgattaaac gcctgattgg ccgccgcttc caggacgaag aagtacagcg tgatgtttcc
     2521 atcatgccgt tcaaaattat tgctgctgat aacggcgacg catgggtcga agttaaaggc
     2581 cagaaaatgg caccgccgca gatttctgct gaagtgctga aaaaaatgaa gaaaaccgct
     2641 gaagattacc tgggtgaacc ggtaactgaa gctgttatca ccgtaccggc atactttaac
     2701 gatgctcagc gtcaggcaac caaagacgca ggccgtatcg ctggtctgga agtaaaacgt
     2761 atcatcaacg aaccgaccgc agctgcgttg gcttacggtc tggacaaagg cactggcaac
     2821 cgtactatcg cggtttatga cctggggggt ggtactttcg atatttctat tatcgaaatc
     2881 gacgaagttg acggcgaaaa aaccttcgaa gttctggcaa ccaacggtga tacccacctg
     2941 ggtggtgaag acttcgacag ccgtctgatc aactatctgg ttgaagaatt caagaaagat
     3001 cagggcattg acctgcgcaa cgatccgctg gcaatgcagc gcctgaaaga agcggcagaa
     3061 aaagcgaaaa tcgaactgtc ttccgctcag cagaccgacg ttaacctgcc atacatcact
     3121 gcagacgcga ccggtccgaa acacatgaac atcaaagtga ctcgtgcgaa actggaaagc
     3181 ctggttgaag atctggtaaa ccgttccatt gagccgctga aagttgcact gcaggatgct
     3241 ggcctgtccg tatctgatat cgacgacgtt atcctcgttg gtggtcagac tcgtatgcca
     3301 atggttcaga agaaagttgc tgagttcttt ggtaaagagc cgcgtaaaga cgttaacccg
     3361 gacgaagctg tagcaatcgg tgctgctgtt cagggtggtg ttctgactgg cgacgtaaaa
     3421 gacgtactgc tgctggacgt taccccgctg tctctgggta tcgaaaccat gggcggtgtg
     3481 atgacgacgc tgatcgcgaa aaacaccact atcccgacca agcacagcca ggtgttctct
     3541 accgctgaag acaaccagtc tgcggtaacc atccatgtgc tgcagggtga acgtaaacgt
     3601 gcggctgata acaaatctct gggtcagttc aacctagatg gtatcaaccc ggcaccgcgc
     3661 ggcatgccgc agatcgaagt taccttcgat atcgatgctg acggtatcct gcacgtttcc
     3721 gcgaaagata aaaacagcgg taaagagcag aagatcacca tcaaggcttc ttctggtctg
     3781 aacgaagatg aaatccagaa aatggtacgc gacgcagaag ctaacgccga agctgaccgt
     3841 aagtttgaag agctggtaca gactcgcaac cagggcgacc atctgctgca cagcacccgt
     3901 aagcaggttg aagaagcagg cgacaaactg ccggctgacg acaaaactgc tatcgagtct
     3961 gcgctgactg cactggaaac tgctctgaaa ggtgaagaca aagccgctat cgaagcgaaa
     4021 atgcaggaac tggcacaggt ttcccagaaa ctgatggaaa tcgcccagca gcaacatgcc
     4081 cagcagcaga ctgccggtgc tgatgcttct gcaaacaacg cgaaagatga cgatgttgtc
     4141 gacgctgaat ttgaagaagt caaagacaaa aaataatcgc cctataaacg ggtaattata
     4201 ctgacacggg cgaaggggaa tttcctctcc gcccgtgcat tcatctaggg gcaatttaaa
     4261 aaagatggct aagcaagatt attacgagat tttaggcgtt tccaaaacag cggaagagcg
     4321 tgaaatcaga aaggcctaca aacgcctggc catgaaatac cacccggacc gtaaccaggg
     4381 tgacaaagag gccgaggcga aatttaaaga gatcaaggaa gcttatgaag ttctgaccga
     4441 ctcgcaaaaa cgtgcggcat acgatcagta tggtcatgct gcgtttgagc aaggaggcat
     4501 gggcggcggt ggttttggcg gcggcgcaga cttcagcgat atttttggtg acgttttcgg
     4561 cgatattttt ggcggcggac gtggtcgtca acgtgcggcg cgcggtgctg atttacgcta
     4621 taacatggag ctcaccctcg aagaagctgt acgtggcgtg accaaagaga tccgcattcc
     4681 gactctggaa gagtgtgacg tttgccacgg tagcggtgca aaaccaggta cacagccgca
     4741 gacctgtccg acctgtcatg gttctggtca ggtgcagatg cgccagggat tcttcgctgt
     4801 acagcagacc tgtccacact gtcagggccg cggtacgctg atcaaagatc cgtgcaacaa
     4861 atgtcatggt catggtcgtg ttgagcgcag caaaacgctg tccgttaaaa tcccggcagg
     4921 ggtggacact ggagaccgca tccgtcttgc gggcgaaggt gaagcgggcg agcatggcgc
     4981 accggcaggc gatctgtacg ttcaggttca ggttaaacag cacccgattt tcgagcgtga
     5041 aggcaacaac ctgtattgcg aagtcccgat caacttcgct atggcggcgc tgggtggcga
     5101 aatcgaagta ccgacccttg atggtcgcgt caaactgaaa gtgcctggcg aaacccagac
     5161 cggtaagcta ttccgtatgc gcggtaaagg cgtcaagtct gtccgcggtg gcgcacaggg
     5221 tgatttgctg tgccgcgttg tcgtcgaaac accggtaggc ctgaacgaaa agcagaaaca
     5281 gctgctgcaa gagctgcaag aaagcttcgg tggcccaacc ggcgagcaca acagcccgcg
     5341 ctcaaagagc ttctttgatg gtgtgaagaa gttttttgac gacctgaccc gctaacctcc
     5401 ccaaaagcct gcccgtgggc aggcctgggt aaaaataggg tgcgttgaag atatgcgagc
     5461 acctgtaaag tggcggggat cactccccgc cgttgctctt actcggattc gtaagccgtg
     5521 aaaacagcaa cctccgtctg gccagttcgg atgtgaacct cacagaggtc ttttctcgtt
     5581 accagcgccg ccactacggc ggtgatacag atgacgatca gggcgacaat catcgcctta
     5641 tgctgcttca ttgctctctt ctccttgacc ttacggtcag taagaggcac tc
//
LOCUS       DAJTQY010000174         5650 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.174, whole genome shotgun sequence.
ACCESSION   DAJTQY010000174 DAJTQY010000000
VERSION     DAJTQY010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5650
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.174"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            850..3135
                     /gene="nrdA"
                     /locus_tag="OO349_003830"
     CDS             850..3135
                     /gene="nrdA"
                     /locus_tag="OO349_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HCR5868734.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPTIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            3369..4499
                     /gene="nrdB"
                     /locus_tag="OO349_003831"
     CDS             3369..4499
                     /gene="nrdB"
                     /locus_tag="OO349_003831"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HCR5868735.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            4499..4753
                     /gene="yfaE"
                     /locus_tag="OO349_003832"
     CDS             4499..4753
                     /gene="yfaE"
                     /locus_tag="OO349_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HCR5868736.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEL"
     gene            complement(4807..5457)
                     /gene="inaA"
                     /locus_tag="OO349_003833"
     CDS             complement(4807..5457)
                     /gene="inaA"
                     /locus_tag="OO349_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HCR5868737.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYARHAVSPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
BASE COUNT         1448 a         1479 c         1344 g         1379 t
ORIGIN      
        1 ctgaaaggct ttgcctgctg gcgtgacaac tcgctttgat atcatatccc tggcataaat
       61 caatggccgc cgcgacaccg ttagcagaga aaagagatgc aatcattgac gggagtagag
      121 ataaatactc cttgcataga aagataatcc gcatattaat ctgaccatca ttttatataa
      181 gacggcatat ttaaacgcat tatacaatat gggttatctt tcttttgtaa attgtccaac
      241 ccgatgcaat ggctattttg ttattatttg atgaataata tcagtgcgtc ataattcaag
      301 ttaataacct taagggatac cagttatatt taaactaaat taaagtaatg aataattttc
      361 ttataatata aggtaaatta acaaaatggc ttcgcattta acaataaccg aatagaaaac
      421 aaccatttcg acatcaacaa tcgcttacat tcgcttatat attgaccaca actgatacat
      481 cagattatgt gatgactcgt gcttagatca atttttgcaa tcattagcaa aaagattaat
      541 aaaccatcta tatcaattta tccaacctat tctgccgttc aagaaatcgc cgaacagtta
      601 tttttaacaa atttttctct tcccattgac tttcccggac accttgtctg acctaaggtg
      661 cgcgaaagcc actttttcct tcctgagtta tccacaaagt tatgcacttg caagagggtc
      721 attttcacac tatcttgcag tgaatcccaa acataccccc tatatatagt gttctaagca
      781 gcttccctta ctacaggtag tctgcatgaa actattgcgg aaagaattcc aaaaacaggt
      841 acgacataca tgaatcagaa tctgctggtg acaaagcgcg acggtagcac agagcgcatc
      901 aatctcgaca aaatccatcg cgttctggat tgggcggcag aaggactgca taacgtttcg
      961 atttcccagg tcgagctgcg ctcccacatt cagttttatg acggtatcaa gacctctgac
     1021 atccacgaaa ctattatcaa ggctgccgca gacctgatct cccgtgatgc gccggattat
     1081 cagtatctcg ccgcgcgcct ggcgatcttc cacctgcgta aaaaagccta cggccagttt
     1141 gagccgcctg cgctgtacga ccacgtggtg aaaatggtcg agatgggcaa atacgataat
     1201 catctgctgg aagactacac ggaagaagag ttcaagcaga tggacacgtt tatcgatcac
     1261 gaccgtgata tgaccttctc ttatgctgcc gttaagcagc tggaaggcaa atatctagta
     1321 cagaaccgcg tgaccggcga aatctacgaa agcgcccagt tcctttatat tcttgttgcc
     1381 gcgtgcctgt tctcgaacta cccgcgtgaa acgcgcctgc aatacgtgaa gcgtttttat
     1441 gacgcggttt ccacatttaa aatttcgctg ccgacgccaa tcatgtccgg cgtgcgtacc
     1501 ccgactcgtc agttcagctc ctgcgtactg atcgagtgcg gtgacagcct ggattccatc
     1561 aacgccacct ccagcgcgat tgtgaaatat gtttcccagc gtgccgggat cggcatcaac
     1621 gccggacgta ttcgtgcgct gggcagcccg attcgcggtg gcgaagcgtt ccataccggc
     1681 tgtatcccgt tctacaaaca tttccagaca gcggtgaaat cctgctctca gggcggtgtg
     1741 cgcggcggtg cggcaacgct gttctacccg atgtggcatc tggaagtgga aagcctgctg
     1801 gtgttgaaaa acaaccgtgg ggttgaaggc aaccgcgtgc gtcatatgga ctacggggta
     1861 caaatcaaca aactgatgta tacccgcctg ctgaaaggtg aagatatcac cctgttcagc
     1921 ccgtccgacg taccggggct gtacgacgcg ttcttcgccg atcaggaaga gtttgaacgc
     1981 ctgtatacca aatatgagaa agacgacagc atccgcaagc agcgtgtgaa agccgttgag
     2041 ctgttctcgc tgatgatgca ggaacgtgcg tctaccggtc gtatctatat tcagaacgtt
     2101 gaccactgta atacccatag tccgtttgat ccgaccatcg cgccagttcg ccagtctaac
     2161 ctgtgcctgg aaattgccct gccgaccaaa ccgctgaacg acgtcaacga cgagaacggt
     2221 gaaatcgcgc tgtgtacgct gtctgctttc aacctgggcg caattaatag cctggatgaa
     2281 ctggaagagc tggcgattct ggcggttcgt gcacttgacg cgctactgga ttatcaggat
     2341 tacccgatcc cggccgccaa acgtggagcg atggggcgtc gtacgctggg tattggtgtg
     2401 attaacttcg cttactatct ggcgaagcac ggtaaacgct actccgacgg cagcgccaac
     2461 aacctgacgc ataaaacctt cgaagcgatt cagtattacc tgctgaaagc gtctaatgag
     2521 ctggctaaag agcaaggcgc gtgcccgtgg tttaacgaaa ccacttacgc gaaagggatc
     2581 ctgccgatcg atacctataa gaaagatctg gatgccatcg ctaatgagcc gctgcattac
     2641 gactgggaag cattgcgtga atcaatcaaa acgcacggcc tgcgtaactc cacgctttct
     2701 gccctgatgc cgtccgagac ctcttcgcag atctctaacg ccactaacgg tattgaaccg
     2761 ccgcgcggtt acgtcagcat caaagcgtcg aaagacggta ttttgcgcca ggtggtgccg
     2821 gactacgagc acctgcacga cgcctatgag ctgctgtggg aaatgccggg taacgatggc
     2881 tacctgcaac tggtaggtat catgcagaaa tttatcgatc agtcgatctc tgctaacacc
     2941 aactacgatc cgtcacgttt cccgtcagga aaagtgccga tgcagcagtt gctgaaagac
     3001 ctgctcaccg cctacaaatt cggggtcaaa acgctgtatt atcagaacac ccgtgacggc
     3061 gctgaagacg cgcaggacga tctggtaccg tcaatccagg acgatggctg cgaaagcggc
     3121 gcatgtaaga tctgatattg agatgccgga tgcggcgtaa acgccttatc cggcctacgg
     3181 ctcggtttgt aggcctgata agacgcgcca gcgtcgcatc aggctccggg tgccggatgc
     3241 agcgtgaacg ccttatccgg cctacggctc ggatttgtag gcctgataag acgcgccagc
     3301 gtcgcatcag gcacaggatg cggcgtaaaa tgccttatcc ggcattaaac tcccaacagg
     3361 acacactcat ggcatatacc accttttcac agacgaaaaa tgatcagctc aaagaaccga
     3421 tgttctttgg tcagccggtc aacgtggctc gctacgatca gcaaaaatat gacatcttcg
     3481 aaaagctgat cgaaaagcag ctctctttct tctggcgtcc ggaagaagtt gacgtctccc
     3541 gcgaccgtat agattaccag gcgctgccgg agcacgaaaa acacatcttt atcagcaacc
     3601 tgaaatatca gacgctgctg gattccattc agggtcgtag cccgaacgtg gcgctattgc
     3661 cgcttatttc tattccggaa ctggaaacct gggtcgaaac ctgggcgttc tcagaaacga
     3721 ttcattcccg ttcctatact catatcattc gtaatatcgt taacgatccg tctgttgtgt
     3781 ttgacgatat cgtcaccaac gagcagatcc agaaacgtgc ggaagggatc tccagctatt
     3841 acgatgaact gatcgaaatg accagctact ggcaccttct gggcgaaggc acccacaccg
     3901 ttaacggtaa aaccgtaacc gttagcctgc gcgagctgaa gaaaaaactg tatctctgcc
     3961 tgatgagcgt taacgcgctg gaagcgatcc gcttctacgt cagctttgct tgttcctttg
     4021 cctttgcaga acgcgaactg atggaaggta acgccaagat tattcgcctg attgcccgcg
     4081 atgaagccct gcacctgacc ggtactcagc atatgctgaa tctgctgcgc agcggcgcgg
     4141 atgatccgga gatggcggaa attgccgaag aatgtaagca ggagtgctat gacctgtttg
     4201 tccaggcggc acagcaagag aaagactggg cggattatct gttccgtgac ggttcgatga
     4261 ttggtctgaa taaagatatt ctctgccagt acgttgaata catcaccaat atccgcatgc
     4321 aggcagtcgg tttggatctg ccgttccaga cgcgctccaa cccgatcccg tggatcaaca
     4381 cctggctggt gtctgataac gtgcaggttg ctccgcagga agtggaagtc agttcttatc
     4441 tggtcgggca gattgacgca gaagtggaca ccgacgattt gagtaacttc cagctctgat
     4501 ggcccgcgtt accctgcgca tcactggcac acaactgctg tgccaggatg aacacccttc
     4561 ccttctggcg gcgctggaat cccacaatgt ggcggttgag taccagtgcc gcgaaggtta
     4621 ctgcggctcc tgtcgcaccc gactggtcgc aggtcaggtg gactggattg ccgaaccgtt
     4681 agcctttatt cagccggggg aaattttgcc ctgttgttgc cgggcaaaag gcgatattga
     4741 aatcgagttg tgatcgggta ctagcgccag atacgacgct tacgcgtatt atctggccta
     4801 caacgattac atggcgtagt aatacgcttt cacctgctcc cagtccgctt tagggatcgg
     4861 ctccagatat ttttccagct ggcggaagtc atgattaatc gctttatcgc gacgcaagcg
     4921 gcgacgactt ttctccagat caagaaaacc agcttctgcc ttaccttctg ttttcacata
     4981 gatatggcga acatagcaac agccatgctg acgattaacg ctatgcattt tcttaaacgc
     5041 cagtgccact gctttcaaca tggcttgccg tacttcgtca gaataaggcg atactgcatg
     5101 ccgggcatac cagtcagcaa tgctgatgaa ccccgccata tcttcagtca ccagcaacgc
     5161 gcgccattca ccctcaattt tcaccgcttc accaaaaacg atcttcggca cgatgacacc
     5221 ggcccgttcc agttctttaa ttaccgcaac ctcacggaca atcgttggtc ggccgaacgg
     5281 ataacgtact gaatgaaaca gatgatgcgt catgcgcttt acatacagct ttttgccgtt
     5341 gcgctcgacg cattgcaccc cgctcatacc attacggcga tagttaggct cttcaaccca
     5401 gtcgccctct gttgcccacc agtgattaaa ttcgtcgtac tttgctgaaa ctgccatacc
     5461 ctatcgcctg tcatttttat taacgacaat gactataggt ggttacctga ggaaaatctt
     5521 aatgaaacgt gtcgtattaa tgaatttctt ttgccgctac aggtgtgaca ccgcttttgc
     5581 ccttcatttg cagcgcatag attccaccag ccactaccac taatagtgcg cccagcagat
     5641 aaaatgtgaa
//
LOCUS       DAJTQY010000175         5579 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.175, whole genome shotgun sequence.
ACCESSION   DAJTQY010000175 DAJTQY010000000
VERSION     DAJTQY010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5579
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.175"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(1..279)
                     /locus_tag="OO349_003834"
     CDS             complement(1..279)
                     /locus_tag="OO349_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5868738.1"
                     /translation="MAHDTKFYNSDNSAAPASRHGRRSHAFKSDGYQHDPCTEEQAEW
                     LIQRYRRHGYEIKKALSLDYRHWIIFVRLPYSERPPRPSCTFQQRIWR"
     gene            220..405
                     /locus_tag="OO349_003835"
     CDS             220..405
                     /locus_tag="OO349_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5868739.1"
                     /translation="MPAGRGGRVIRIVKFGIVRHLFSLLAQQVPVVQADGWIVNQNDQ
                     KKQNPPKRVKCGCVEDA"
     gene            complement(447..602)
                     /gene="hokD"
                     /locus_tag="OO349_003836"
     CDS             complement(447..602)
                     /gene="hokD"
                     /locus_tag="OO349_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HCR5868740.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            complement(1254..1670)
                     /locus_tag="OO349_003837"
     CDS             complement(1254..1670)
                     /locus_tag="OO349_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5868741.1"
                     /translation="MTELTKEQLIEEAKLKIAITKCHPNSGMARVEGELFKIARASLE
                     AEPIAWRYRYVKKGVMDSQGELWVGDWKYVPKKEDCNDRPNYEIQALFTAPPVPVTSE
                     ELVKAVHFYEQLKRENPPASGNQINGLTMSVKRPAN"
     gene            1697..1837
                     /locus_tag="OO349_003838"
                     /pseudo
     CDS             1697..1837
                     /locus_tag="OO349_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
     gene            1837..2877
                     /locus_tag="OO349_003839"
     CDS             1837..2877
                     /locus_tag="OO349_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5868742.1"
                     /translation="MGRRRKNPEHEKLPPNVYPNKYSYVWKPTSRESVTLTAIKDGLA
                     ALWKKYEETVNNRDRAMTFGRLWEKFLASAYYSDLSPRTQKDYLQHQKKLLAVFGKVP
                     ADSIKPEHIRRYMDKRGEQSKTQANHEKSSMSRVYSWGYERGYVKANPCAGVSKFKAK
                     NRERYVTDKEYQAVLSVAPLPVFIAMEIAYLCAARVSDVLSLKWEQIGNDGIFIQQGK
                     TGKKQIKAWSPRLQAAIEKAKQLPTSAYVISNQYGNRYMYKGFNEMWVEARNRAGKIS
                     GILTDFTFHDLKAKGISDYEGSSRDKQLFSGHKTEGQVLIYDRKVKVSPTLDVPLPEN
                     IPRKYSKCDFCH"
     gene            complement(2905..2994)
                     /locus_tag="OO349_003840"
     tRNA            complement(2905..2994)
                     /locus_tag="OO349_003840"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2958..2960),aa:Ser,seq:cga)
     gene            3088..3885
                     /gene="mtfA"
                     /locus_tag="OO349_003841"
     CDS             3088..3885
                     /gene="mtfA"
                     /locus_tag="OO349_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HCR5868743.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVALAE
                     RFLQQKRLVPLQGFELNSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYVASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            3986..4061
                     /locus_tag="OO349_003842"
     tRNA            3986..4061
                     /locus_tag="OO349_003842"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4019..4021,aa:Asn,seq:gtt)
     gene            4223..5485
                     /locus_tag="OO349_003843"
                     /pseudo
     CDS             4223..5485
                     /locus_tag="OO349_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT         1504 a         1323 c         1292 g         1460 t
ORIGIN      
        1 ttacctccag atccgttgct ggaatgtgca ggacggacgc ggtggccgtt cggaataagg
       61 gagcctgacg aagattatcc agtgacgata atcgagactg agggctttct taatctcgta
      121 tccgtgtctg cggtagcgct gaatcagcca ttcagcctgt tcttcggtgc aggggtcgtg
      181 ctgatacccg tcagatttga atgcatgaga acgccgcccg tgcctgctgg caggggcggc
      241 agagttatcc gaattgtaaa atttggtatc gtgcgccatc tgttttctct gctggcgcag
      301 caggtgccag ttgttcaggc tgacggatgg attgtaaacc agaacgacca gaaaaaacaa
      361 aacccgccga agcgggttaa gtgcgggtgc gttgaggatg cctgacacat cagcggtggc
      421 gagggatttc tcccccgccg ggtctcttac tcctcaggtt cgtaagctgt gaagacagcg
      481 acctccgtct ggccggttcg gattcgtacc tcgcagaggt ctttcctcgt taccagtgcc
      541 gtcactatga cggttaaaca gatgacgata agggcgatta acatcgcctt ttgctgcttc
      601 atagcctgct tctccttgcc tttcggcacg taagaggcta acctacatgt gttcagcatg
      661 gattgagcct cagattaatg ttaagcgtct tgcaggacgc gtaatgttaa ctggggcttt
      721 tctctatctg ccttttggtg ttcatgcctg agacagatag cctcaagcac ccgcagtcat
      781 tctacttaac taagatttcc ctgcaaaccg tttttgtccg gcacagtaaa tatccaacta
      841 aaccaatagc gttcgctgta tttaccgcca gtattcaatg cacatgaccg ccatgaacac
      901 ccctaaaaaa agggcattta tatgtccaaa cattaatatc aaaacatcaa ttttttccat
      961 ataccttgct gtgaagatga tgggcataca tgatgcgaac aaccagaacg caacaaacaa
     1021 aaactgcaat gcgtttttca ttattccccc tacaatcaat gtgcaataac atttaaacac
     1081 acctcaattt ggccggacat ataaatatct aaaccagaaa aaatcactta catagcgtta
     1141 caaactcttt agtctaaata ctcatcgtaa aacattcccc atacttatca gtccgttccg
     1201 cgccaggtag cttattgcct tatctggcaa cctataatca ggtttccgct ttttcagttg
     1261 gctggtcgtt taaccgacat agttaaccca ttaatctggt tgccggatgc tggtggattt
     1321 tcgcgtttta attgttcata aaagtgcaca gctttaacca gttcttctga tgtaaccggg
     1381 actggtgggg cagtgaataa ggcctgaatt tcatagttcg gcctgtcgtt acaatcctct
     1441 tttttcggta catatttcca gtcaccaacc cacaactccc cctgagagtc cataacacct
     1501 tttttcacgt agcgatatcg ccacgctatc ggctctgctt ccagcgatgc ccgtgcaatt
     1561 ttgaataact cgccctctac tcgcgccatc cctgaattgg ggtggcattt cgtaatcgct
     1621 atttttaatt tggcttcttc gattaattgt tcttttgtta attcagtcat tttcattacc
     1681 gccctggtaa tgctgccacc agcgaaaatg gttaatggaa aacgggcttc cgttctatac
     1741 caatcgtagt ggcaaaccga ttgtcagccg ggatctattt acctgcaata aaactttacc
     1801 accacgcgag gtagagccga attttggtgc gatctgatgg gaagacgaag gaaaaatcct
     1861 gaacacgaaa aattacctcc aaatgtatac ccaaataaat atagttatgt atggaaacca
     1921 acatccagag aatctgtaac actaaccgcc atcaaggatg gtttagctgc tttatggaaa
     1981 aagtatgagg aaactgtaaa taatcgcgat cgtgcaatga cattcggtcg cttgtgggaa
     2041 aaattcctcg ccagcgccta ttacagtgac ctcagtccaa gaacacaaaa agattatctg
     2101 caacatcaaa aaaagttgct tgccgtattc ggtaaggtgc cggcagattc cataaaacca
     2161 gaacacatcc gtcgatacat ggacaagaga ggggaacaga gtaaaacgca agccaaccat
     2221 gaaaaaagca gtatgtcccg cgtttacagt tgggggtatg agcgagggta cgtgaaggct
     2281 aacccatgtg caggtgtaag taaattcaag gccaaaaacc gcgaacgata tgtaaccgac
     2341 aaagaatacc aggcagtatt aagcgttgca cctcttcctg tttttatcgc aatggaaatt
     2401 gcctatctgt gtgcagcgag ggtttccgat gtgttatcgc tgaaatggga gcagattgga
     2461 aacgacggga tctttatcca gcaagggaaa acaggaaaaa aacagataaa agcatggagt
     2521 ccacgattac aggcggcgat cgaaaaagca aaacagttac caacatccgc ctatgtaatc
     2581 agcaatcaat acggcaaccg atatatgtac aaaggcttta acgaaatgtg ggtagaagca
     2641 agaaatcgcg caggcaaaat ttcaggtatt ttaaccgact tcacctttca tgatctgaag
     2701 gcgaaaggaa tttcagacta tgaaggaagc agtcgggata agcaactttt ctctggtcac
     2761 aaaaccgagg ggcaagtgct aatctatgac aggaaggtta aagtttcacc gacacttgat
     2821 gtcccgttac ctgaaaatat tccaagaaaa tattccaagt gtgatttttg tcactgactt
     2881 aatgatgtat aagtgattga attttggcgg agagaggggg atttgaaccc ccggtggagt
     2941 tgcccccact ccggttttcg agaccggtcc gttcagccgc tccggcatct ctccgttcag
     3001 atggttgtca tgatgccagg aaatttggca ttttaacagt ccctgtccgt gcaattttgt
     3061 tcaagtgacg agtttgcgag caaaacgatg attaagtggc cctggaaagt acaagaatca
     3121 gcacatcaaa ctgcccttcc ctggcaggaa gcactatcga tccccctttt aacgtgtctg
     3181 acagaacagg aacaaagcaa attggtcgct cttgccgaac gttttttaca gcaaaaacgg
     3241 cttgttcctt tacagggctt tgagctgaat tcattaagaa gctgccggat agcacttcta
     3301 ttttgcctgc ccgttctgga gttaggactg gaatggctgg atggttttca tgaagtctta
     3361 atttatcctg cgccatttgt ggtcgatgat gaatgggaag acgatatcgg tctggtgcat
     3421 aaccaacgta ttgttcagtc aggtcagagc tggcagcaag ggcctatcgt tttgaactgg
     3481 ttggatatac aagattcttt tgatgcttct ggttttaacc tgattattca tgaagtcgct
     3541 cataagctgg acacccgtaa cggcgatcgc gccagcggag ttccctttat ttcgttgcgt
     3601 gaggttgctg gctgggaaca cgatcttcat gctgcaatga acaacattca ggaagaaatc
     3661 gaattagttg gtgagaatgc ggcgagcatt gatgcttatg ttgccagtga tcctgctgaa
     3721 tgttttgccg tactttctga atatttcttt agcgccccag aactttttgc tcctcgtttc
     3781 ccttcattgt ggcaacgttt ctgtcaattt tatcaacaag atcctttgca gagactgcat
     3841 cacgctaatg atacagactc gttttcagcg acgaatgttc attaactaac aactttgaag
     3901 attaattaac caattgaaat ggcttatgaa atttagtgtt gacagacaag gtaccgctaa
     3961 gtaatatgcg ccccgttctc acgattcctc tgtagttcag tcggtagaac ggcggactgt
     4021 taatccgtat gtcactggtt cgagtccagt cagaggagcc aattttctgt tttcatgcat
     4081 ccttgcgaat ctttatctga tgttgattca acaggttagt gtgaaaactc ttctcggtgc
     4141 attttgattt taccctacgc atcgggaaaa attggtggtc aaatctggga tcaggttagt
     4201 tcgataatgg agtgaccccc atatatccct taccgacgca aaaatccgca ccctcaagcc
     4261 ttctgataaa ccctttaaag tctccgattc tcacggtctg tatctgctgg tcaagccggg
     4321 tggctcccgc cactggtatc tcaaataccg tattagcggt aaagaatccc gcattgcgct
     4381 gggtgcctat ccagccatct ccctgtctga tgcgcgacag caacgtgaag gtatccgtaa
     4441 aatgctggcg ctgaatatca acccggtaca gcagcgggct gctgaacgtg gctcacgaac
     4501 accggagaaa gtttttaaaa acatggcgct ggcgtgacat aaaagtaaca ggaaatggtc
     4561 gcagaacacc gccgaccgtc tgcttgccag cctgaacaat cacatctttc cggtcatcgg
     4621 gaacctacct gtatcagaac ttaaaccccg tcatttcatt gacctgctga aagggatcga
     4681 ggaaaaaggt ctgctggagg ttgcgtcccg cacacggcag cacctgagta acataatgcg
     4741 ccatgcggtc catcaggagt taatcgatac gaaccctgca gcaaaccttg gcggcgtgac
     4801 cacacctcct gtcagacggc actatcctgc cctgccgctg gagcggctgc ctgaactgct
     4861 tgaacgtatt ggggcatatc atcagggccg tgaactgacc cggcatgccg ttctgctgat
     4921 gctgcatgtg ttcattcgct ccagtgaact gcgtttcgcc cgctggtcag agattgattt
     4981 cacaaaccga gtctggacga tacccgcgac gcgagaaccc attattggcg tgcgttattc
     5041 cggccgcggg gcaaaaatgc gaatgccgca tatcgtcccc ctctcagaac agtccatcgc
     5101 cattctgaaa cagattaagg atatcaccgg taataatgaa ctgatcttcc ccggcgacca
     5161 taacccgtat aagccaatgt gtgaaaacac ggtcaataag gcactgcggg tgatgggtta
     5221 cgacacgaaa aaggatatct gcggtcacgg cttccgggca atggcatgca gtgcgctgat
     5281 ggaatcgggt ttatgggcaa aggacgcagt agaacgccag atgagtcatc aggagcgcaa
     5341 taccgtgcgc atggcttata ttcataaggc agagcacctg gaagcccgca aagcgatgat
     5401 gcagtggtgg tcggattatc tggaagcatg ccgagaatct tatgcaccgc cttatacaat
     5461 tggtaaaaat aagtttatcc catagtatac taacgaatcc cacactctgg caaattacca
     5521 ccacctctac aggaggtggt tctatataga cttttatcaa cttcatgacg aaaaacacc
//
LOCUS       DAJTQY010000176         5559 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.176, whole genome shotgun sequence.
ACCESSION   DAJTQY010000176 DAJTQY010000000
VERSION     DAJTQY010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5559
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.176"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            <1..643
                     /locus_tag="OO349_003844"
     CDS             <1..643
                     /locus_tag="OO349_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
                     /protein_id="HCR5868744.1"
                     /translation="VVGACIVLMELMMMVQLWWSVWLCWPVLSLLIIGRGCGGLGAIT
                     TGKDSQGKLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLRAFPRHIPAQN
                     AVLDVTFEFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCA
                     LGLSRSALVVAAWLLCYGHCKTVDEAIIFIRARRSHIVLKEEHKAMLKLWENR"
     gene            complement(697..1302)
                     /gene="azoR"
                     /locus_tag="OO349_003845"
     CDS             complement(697..1302)
                     /gene="azoR"
                     /locus_tag="OO349_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HCR5868745.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWSEKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            1503..5405
                     /gene="hrpA"
                     /locus_tag="OO349_003846"
     CDS             1503..5405
                     /gene="hrpA"
                     /locus_tag="OO349_003846"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HCR5868746.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHCVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DSALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVTIKLFDNPLEQKQAMWNGLRRLLLLNISSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
BASE COUNT         1377 a         1359 c         1540 g         1283 t
ORIGIN      
        1 tgtcgtaggc gcgtgcattg tgttgatgga gctaatgatg atggttcagt tatggtggtc
       61 agtctggtta tgttggccag tattatcgct actcattatt ggccgtgggt gcggtgggct
      121 tggcgcgata acaacaggga aagatagtca ggggaaactc ccgcccgccg tttactggct
      181 gacattgccc tggcgcatcg ggatgtggct atctatgcgt tggttttgtc gtcgcctgga
      241 gccggtgagc aaaatgactg ctggtgttta tttaagggcg tttccacgac atattccggc
      301 acagaatgcg gttctggacg tcacctttga attccctcgt ggacgagcga caaaagatcg
      361 actctatttc tgtgtaccga tgttggatct ggtggttccg gaagaggggg agctccgaca
      421 ggccgtggcg atgctggaaa cattacgcga agagcaaggc agcgttctgg tccattgcgc
      481 attgggatta tcgcgcagtg cgctggtagt ggcggcatgg ctgttatgtt acggacactg
      541 taaaacagtt gatgaagcga ttatctttat tcgagccaga cgctcgcata ttgtgcttaa
      601 ggaagagcac aaagcgatgt tgaaattatg ggaaaacagg taagtggatt gagatgtgga
      661 ctgaatatct acagtccaca tcaagaccgt gtccggttat gcagcaacaa tgctgtcgat
      721 ggctgctttt gcgtcagact gtgctttcgc tgccatttcc ggaccgtatg cgatcccttc
      781 ggcgaagaca aatttcacat cggtaatgcc gataaagccg aggaacgtgg acagatacgg
      841 cgtcaccagg tccgttggtc catctttgtg gatcccaccg cggctggtaa taacgatggc
      901 ttttttaccc gttaccagac cttccggacc gttctcggta tagcggaaag taacgcctgc
      961 gcgtgcaacc aggtcaaaat agtttttcaa ctgagttgag atgttgaagt tatacatcgg
     1021 tgccgcaata acgataacgt cgtgggcttt cagctcggca atcagctcat cggaaagcgc
     1081 cagagcttcc tgctgacgcg gagtcagcgg cgcatcgctc ggacgcagag cgccaaccag
     1141 ttcgccatcc agtaccggaa tcggatttgc agctaggtcg cgaacggtga tttcatcagc
     1201 ggagtgcttt tcgctccatt gttcaacaaa ataatcggac aactgattag actgagagta
     1261 ccctgccagg atgctggatt taagaactaa taccttgctc atggtgtttc cttatagatg
     1321 tttgaatgga cgatgccccg ttgcttgctg acactttatt cacaatcctg ccacagagat
     1381 agcgcaataa atcgaagtct atgttcgaat ttattgaaca acgcatagaa agccgcgatg
     1441 tggtactcta tatctatcat ttaaaagaaa attaatcagg cagactactg cccactaacg
     1501 ttatgacaga acaacaaaaa ttgaccttta ctgccttgca acagcggctg gattcgctga
     1561 tgctgcgcga cagactgcgt ttttctcgcc gtctgcattg tgttaagaag gttaaaaatc
     1621 ctgatgcaca acaggccatt ttccaggaga tggcgaaaga gattgaccag gcggcaggga
     1681 aagtcctgct gcgtgaagcg gcacgaccgg aaatcactta tcctgacaat ttaccggtta
     1741 gtcagaaaaa acaggacata ctcgaagcga ttcgtgatca ccaggtggtg atcgtcgccg
     1801 gggaaaccgg ctccggtaaa acgactcagc taccgaaaat ctgtatggag ctggggcgcg
     1861 gtattaaagg gctgatcggt catacccagc cgcgtcgcct ggcggcacga acggtggcca
     1921 accgtattgc ggaagagctg aaaacggagc cgggcggttg catcggttac aaagtgcgtt
     1981 tcagcgatca cgtaagtgat aacacgatgg tcaagctgat gaccgacggg atcctgctgg
     2041 cggagatcca gcaagaccgc ctgctgatgc agtacgacac catcattatt gacgaagcgc
     2101 acgaacgcag cctgaatatc gattttctgc tcggctattt gaaagagttg ctgccgcggc
     2161 gtcctgacct aaaaatcatt atcacttccg cgactatcga cccggaacgc ttttcgcgcc
     2221 actttaataa tgcgccgatt attgaagtct ccggtcggac ctatccggtg gaagtgcgct
     2281 atcgcccgat tgttgaagaa gccgatgaca ccgagcgcga tcagttgcag gcgatttttg
     2341 acgccgtaga cgaactaagc caggaaagcc ccggcgacat tctgatcttt atgagcggcg
     2401 agcgggaaat tcgcgatacc gccgatgcgc tgaacaagct gaacttacgc cataccgaaa
     2461 tcttgccgct ttatgcgcgg ctttcgaaca gcgaacagaa ccgcgtgttc cagtcgcaca
     2521 gcggacggcg cattgtgctg gcgaccaacg tcgcggaaac ctcgctgacc gtaccgggga
     2581 ttaaatacgt catcgacccc ggtacagcgc gtatcagccg ctacagctat cgcaccaaag
     2641 tgcagcgttt gccgattgaa cccatttcgc aggcgtctgc caatcagcgt aaaggccgct
     2701 gtggtcgtgt gtccgaaggg atctgtattc gtctctattc cgaagacgat ttcctctcgc
     2761 gcccggagtt taccgatccg gagattctgc gtaccaacct ggcctcggtt attttgcaga
     2821 tgaccgcgct ggggctgggc gatatcgctg cgttcccgtt tgtcgaagca ccggataaac
     2881 gcaatatcca ggatggcgtg cgtctgctcg aagaactggg cgcgatcacc acggatgaac
     2941 aggccagcgc ctataaactg acgccgctcg gtcgccagct ctcgcagttg cctgtcgacc
     3001 cacgtctggc gcgtatggtg ctggaagcgc aaaaacatgg ctgcgtgcgt gaggcgatga
     3061 ttatcacgtc cgcgctctcc atccaggatc cgcgcgaacg tccgatggac aaacagcagg
     3121 catcggacga aaaacatcgt cgcttccacg acaaagaatc cgacttcctc gcgtttgtaa
     3181 atctgtggaa ttatcttggc gagcagcaaa aggcgctttc ttccaacgcc ttccgtcgcc
     3241 tgtgtcgtac cgattacctc aactatctgc gcgtgcgcga atggcaggat atctacaccc
     3301 agttgcgcca ggtggtgaaa gaacttggca ttccggtcaa cagcgaaccg gcggagtatc
     3361 gtgaaattca catcgcctta ctgaccggtt tgctttccca tatcggcatg aaagatgccg
     3421 ataaacagga atataccggt gcacgtaacg cgcgtttctc catcttcccc ggttctggtt
     3481 tattcaaaaa gccgccgaaa tgggtaatgg tggcggaact ggtagaaacc agccgcctgt
     3541 gggggcgcat tgctgcgcgt atcgacccgg aatgggtaga acccgttgct cagcatttga
     3601 ttaaacgcac ctacagcgaa ccgcactggg aacgggcgca gggcgcggtg atggcaacgg
     3661 aaaaagtcac tgtttatggt ttgccgattg ttgctgcgcg caaggtcaac tacagccaga
     3721 tcgattcggc gttatgtcgt gaactcttta ttcgccacgc tctggtggaa ggtgactggc
     3781 agacgcgtca cgcattcttc cgtgaaaacc tgaaactgcg ggccgaagtg gaagagctgg
     3841 aacacaaatc acgtcgccgc gatattctgg ttgatgacga aacgctgttt gagttctacg
     3901 accagcgcat cagccacgat gtaatctccg ctcgtcactt cgatagctgg tggaaaaaag
     3961 tcagccgcga aacgcctgat ttgctcaact ttgaaaagag catgttgatc aaagaagggg
     4021 cggaaaaaat cagcaagctg gattacccga acttctggca tcagggcaat ctcaagctgc
     4081 gtttgagcta tcagtttgag ccaggcgcgg acgcggacgg tgtgaccgta catattccgc
     4141 tgccgttact taaccaggtt gaggaaagcg ggtttgaatg gcagatcccc ggtctgcgcc
     4201 gcgaattggt gattgctctg attaaatcgt tgccgaaacc ggtacgccgt aattttgtgc
     4261 ctgcaccaaa ctacgccgaa gcgtttttag gccgcgtcac accgctggag ttaccgttgc
     4321 tcgacagcct tgagcgcgag ttacggcgga tgaccggcgt taccgttgac cgcgaagact
     4381 ggcactggga tcaggtgccc gatcacctga aaatcacctt ccgcgtggtg gatgacaaaa
     4441 acaagaagct aaaagaaggg cgctcgctac aagatctgaa agatgcgctg aaaggcaaag
     4501 tgcaggaaac gctgtccgcg gtggcggatg acggtatcga gcagagcggc ttacatatct
     4561 ggagttttgg tcagctgccg gaaagttacg aacagaagcg tggcaactat aaagtgaagg
     4621 cgtggccggc gctggtggat gaacgcgaca gcgtgacgat caaactgttt gataacccgc
     4681 tggagcaaaa acaggcaatg tggaatggtc ttcgccgtct actgctgcta aatatttcct
     4741 cgccgatcaa gtatttacat gagaagttac caaacaaagc caagctgggg ctgtacttta
     4801 acccgtatgg caaagtgctg gagctgatcg acgactgtat ctcctgcggt gtggatcaac
     4861 tgatcgacgc caatggtggc ccggtctgga cggaagaagg ctttgctgcg ctgcatgaaa
     4921 aagtgcgcgc cgaactgaat gacacggtgg tggatattgc gaagcaggtc gagcaaatcc
     4981 tcactgcggt gttcaatatc aacaaacgtc tgaaagggcg ggtggatatg accatggcac
     5041 tggggctttc tgatattaaa gcgcagatgg gcgggttggt atatcgcggt tttgtcactg
     5101 gtaacggctt caaacggctg ggcgacacgc tgcgatattt gcaggcgatt gaaaaacgac
     5161 tggaaaaact ggcggttgat ccacatcgcg atcgtgcaca gatgctgaaa gtcgaaaacg
     5221 tccagcaggc gtggcagcaa tggatcaaca aactgccgcc tgcacgtcgt gaggatgaag
     5281 acgtgaaaga gatccgttgg atgatagaag agctgcgcgt tagttacttc gctcaacaac
     5341 ttggtacgcc ttatccgatt tcagataagc gtattttgca ggcgatggag cagattagcg
     5401 gttaaccctg ctatttgcct aataaatcta aaacccggta agcatttagc gccgggtttt
     5461 ttattaattc taaaacggta aggacaaaaa ttcaggaatt cagaaaaata caattctcag
     5521 ctgcaagatg aataatgttt atctacagca tttccttaa
//
LOCUS       DAJTQY010000177         5400 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.177, whole genome shotgun sequence.
ACCESSION   DAJTQY010000177 DAJTQY010000000
VERSION     DAJTQY010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5400
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.177"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            <1..497
                     /locus_tag="OO349_003847"
     CDS             <1..497
                     /locus_tag="OO349_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="COX aromatic rich motif-containing protein"
                     /protein_id="HCR5868747.1"
                     /translation="EIAFPANTPVYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHL
                     IANEPGTYDGISASYSGPGFSGMKFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEK
                     LAAPSEYNQVEYFSNVKPDLFADVINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHA
                     ESAH"
     gene            519..2510
                     /gene="cyoB"
                     /locus_tag="OO349_003848"
                     /pseudo
     CDS             519..2510
                     /gene="cyoB"
                     /locus_tag="OO349_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
     gene            2500..3114
                     /locus_tag="OO349_003849"
     CDS             2500..3114
                     /locus_tag="OO349_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HCR5868748.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            3114..3443
                     /locus_tag="OO349_003850"
     CDS             3114..3443
                     /locus_tag="OO349_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HCR5868749.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            3455..4345
                     /gene="cyoE"
                     /locus_tag="OO349_003851"
     CDS             3455..4345
                     /gene="cyoE"
                     /locus_tag="OO349_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HCR5868750.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVTSGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPSVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            4530..4841
                     /locus_tag="OO349_003852"
     CDS             4530..4841
                     /locus_tag="OO349_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCR5868751.1"
                     /translation="MPGKTAALYDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            4976..5374
                     /locus_tag="OO349_003853"
     CDS             4976..5374
                     /locus_tag="OO349_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5868752.1"
                     /translation="MRNSDCAAKALIDRQSGELIGICTFTAYSLERQRVSGVLQGSQP
                     SEIGVVRLVMLGGARKYQKRGFGQDLLCDFFEHVKKIHPALPIKGVYLDADPAAINFY
                     ARLGFVQLSATPNAFGAVPMFLAIQHILAA"
BASE COUNT         1181 a         1342 c         1424 g         1453 t
ORIGIN      
        1 atgaaatcgc tttcccggcg aacactccgg tgtacttcaa agtgacctcc aactccgtga
       61 tgaactcctt cttcattccg cgtctgggta gccagattta tgccatggcc ggtatgcaga
      121 ctcgcctgca tctgatcgcc aacgaacccg gtacttatga cggtatctcc gccagctaca
      181 gcgggccggg cttctcaggc atgaagttca aagctattgc aacaccggat cgcgccgaat
      241 tcgaccaatg ggtcgcaaaa gcgaaacagt cgccgaatac catgtctgac atggctgcgt
      301 tcgaaaaact ggccgcgcct agcgaataca accaggtgga atatttctcc aacgtgaaac
      361 cagacttgtt tgccgatgtg attaacaagt ttatggctca cggtaagagc atggacatga
      421 cccagccaga aggtgagcac agtgcacacg aaggtatgga aggcatggac atgagccacg
      481 cggaatccgc ccattaaagg ggttgaggaa gaataaagat gttcggaaaa ttatcacttg
      541 atgcagtccc gttccatgaa actatcgtca tggttacgat cgctggcatt attttgggag
      601 gtctggcgct cgttggcctg atcacttact tcggtaagtg gacctacctg tggaaagagt
      661 ggctgacctc cgtcgaccat aaacgcctcg gtatcatgta tatcatcgtg gcgattgtga
      721 tgttgctgcg tggttttgct gacgccatta tgatgcgtag ccagcaggct cttgcctcgg
      781 cgggtgaagc gggcttcctg ccacctcacc actacgatca gatcttcacc gcgcacggcg
      841 tgattatgat cttcttcgtg gcgatgcctt tcgttatcgg tctgatgaac ctggtggttc
      901 cgctgcagat cggcgcgcgt gacgttgcgt tcccgttcct caacaactta agcttctggt
      961 ttactgttgt tggcgtgatt ctggttaacg tttctctcgg cgtgggcgaa tttgcgcaga
     1021 ccggctggct ggcctatcca ccgctatcgg gaatagagta cagtccggga gtcggtgttg
     1081 attactggat atggagtctc cagctatccg gtataggtac gacgcttacc ggtatcaact
     1141 tcttcgttac cattctgaag atgcgcgcac cgggcatgac catgttcaag ataccagtat
     1201 ttacctaggc atcactgtgc gcgaacgtac tgattattgc ttccttccca attctgacgg
     1261 ttaccgtcgc gttgttgacc ctggatcgct atctgggcac ccatttcttt accaacgata
     1321 tgggtggcaa catgatgatg tacatcaacc tgatttgggc ctggggccac ccggaagttt
     1381 acatcctgat cctgcctgtt ttcggtgtgt tctccgaaat tgcggcaacc ttctcgcgta
     1441 aacgtctgtt tggttatacc tcgctggtat gggcaaccgt ctgcatcacc gtgctgtcgt
     1501 tcatcgtttg gctgcaccac ttctttacga tgggtgcggg cgcgaacgta aacgccttct
     1561 ttggtatcac cacaatgatt atcgccatcc cgaccggggt gaagatcttc aactggctgt
     1621 tcaccatgta tcagggccgc atcgtgttcc attctgcgat gctgtggact atcggtttta
     1681 tcgtcacctt ctcggtgggc ggtatgactg gcgtgctgct ggcagtaccg ggcgcggact
     1741 tcgttctgca taacagcctg ttcctgattg cgcacttcca taacgtgatc atcggcggcg
     1801 tggtcttcgg ctgcttcgca gggatgacct actggtggcc taaagcgttc ggtttcaaac
     1861 tgaacgaaac ctggggtaaa cgcgcgttct ggttctggat catcggcttc ttcgttgcct
     1921 ttatgccgct gtatgcgctg ggcttcatgg gcatgacccg tcgtttgagc cagcagattg
     1981 acccgcagtt ccacaccatg ctgatgattg cagccagcgg tgcagtactg attgcgctgg
     2041 gtattctctg cctcgttatt cagatgtacg tttctattcg cgaccgcgac cagaaccgtg
     2101 acctgactgg cgacccgtgg ggtggccgta cgctggagtg ggcaacctct tccccgcctc
     2161 cgttctataa ctttgccgta gtgccgcacg ttcacgaacg tgatgcattc tgggaaatga
     2221 aagagaaagg cgaagcgtat aaaaagcctg accactatga agaaattcat atgccgaaaa
     2281 acagcggtgc aggtatcgtc attgcagctt tctccaccat cttcggtttc gccatgatct
     2341 ggcatatctg gtggctggcg attgttggct tcgcaggcat gatcatcacc tggatcgtga
     2401 aaagcttcga cgaggacgtg gattactacg tgccggtggc agaaatcgaa aaactggaaa
     2461 accagcattt cgatgagatt actaaggcag ggctgaaaaa tggcaactga tactttgacg
     2521 cacgcgactg cccacgcgca cgaacacggg caccacgatg caggcggaac caaaatcttc
     2581 ggattttgga tctacctgat gagcgactgc attctgttct caatcttgtt tgctacctat
     2641 gccgttctgg tgaacggcac cgcaggcggc ccgacaggta aggacatttt cgaactgccg
     2701 ttcgttctgg ttgaaacttt cttgctgttg ttcagctcca tcacctacgg catggcggct
     2761 atcgccatgt acaaaaacaa caaaagccag gttatctcct ggctggcgtt gacctggttg
     2821 tttggtgccg gatttatcgg gatggaaatc tatgaattcc atcacctgat tgttaacggc
     2881 atggggccgg atcgcagcgg cttcctgtca gcgttctttg cgctggtcgg cacgcacggt
     2941 ctgcacgtca cttctggtct tatctggatg gcggtgctga tggtgcaaat cgcccgtcgc
     3001 ggcctgacca gcactaaccg tacccgcatc atgtgcctga gcctgttctg gcacttcctg
     3061 gatgtggttt ggatctgtgt gttcactgtt gtttatctga tgggggcgat gtaatgagtc
     3121 attctaccga tcacagcggc gcgtcccatg gcagcgtaaa aacctacatg acaggcttta
     3181 tcctgtcgat cattctgacg gtgattccgt tctggatggt gatgacaggg gctgcctctc
     3241 cggccgtaat tctgggaaca atcctggcaa tggcagtggt acaggttctg gtgcatctgg
     3301 tgtgcttcct gcacatgaat accaaatcag atgaaggctg gaacatgacg gcgtttgtct
     3361 tcaccgtgct aatcatcgct atcctggttg taggctccat ctggattatg tggaacctca
     3421 actacaacat gatgatgcac taagagcggc ggttatgatg tttaagcaat acctgcaagt
     3481 aacgaaacca ggcatcatct ttggcaacct gatctcggtg attgggggat tcctgctggc
     3541 ctcaaagggc agcattgatt atcccctgtt tatctacacg ctggttgggg tgtcactggt
     3601 tgtgacgtcg ggttgtgtgt ttaacaacta catcgacagg gatatcgaca gaaagatgga
     3661 aaggacgaag aatcgggtgc tggttaaagg cctgatctct cctgctgtct cgctggtgta
     3721 cgccaccttg ctgggtattg ctggctttat gctgctgtgg tttggcgcga atccgctggc
     3781 ctgctggctg ggggtgatgg gctttgtggt ttatgtcggc gtctatagcc tgtacatgaa
     3841 acgccactca gtctacggca cattgattgg ttcgctctcc ggcgctgcgc cgtcggtgat
     3901 tggctactgt gcagtaaccg gcgagtttga tagcggcgca gcgatcctgc tggctatctt
     3961 cagcctgtgg cagatgcctc actcctatgc catcgccatt ttccgcttta aggattacca
     4021 ggcggcaaac attccggtat tgccagtggt aaaaggcatt tcggtggcga agaatcacat
     4081 cacgctgtat atcatcgcct ttgccgttgc cacgctgatg ctctctcttg gcggttacgc
     4141 tgggtataaa tatctggtgg tcgccgcggc ggttagcgtc tggtggttag gtatggctct
     4201 gcgcggttat aaagttgctg atgacagaat ctgggcgcgc aagctgttcg gcttctctat
     4261 catcgccatc actgccctct cggtgatgat gtccgttgat tttatggtac cggactcgca
     4321 tacgctgctg gctgctgtgt ggtaacaaaa cctctctatt aaaaaggtgc tacggcacct
     4381 tttttattgt ctgagatcag agatacggct aataaccgta tctcttgaca gagtgagcag
     4441 atgcacataa attaatcaaa cggttgatag ccgtattatg tatgagatcg actccctacg
     4501 atttcattca gttaatcaaa gaagtcataa tgcctggaaa aacagccgct ctatacgatg
     4561 tcgataaaac gctgaaaaat gcgcgggtcg aactcaaaac tagtcccgac gccaaaaaca
     4621 aacttcgtga agctgcgcaa gccgttgggg tagatttaag cgcttttatt ttgagcgctg
     4681 ctatggaacg cgccgaaagt gtgcttgata accaacgtcg ccgtgagctt tcgaatcaaa
     4741 gctgggaact gatgaaccaa ctcatcgctg aacctgctca accgacgctc gccctcaagg
     4801 cgttaatgaa aaggaaaaac agcgatggtc gacaagcatg aagagattac tctgcccata
     4861 gtcctctcct gtaattatca gtctgatatt acttatcctg ggcaaaaaca gtttgattgc
     4921 ggtaaccctg ttatcgataa atttgtacgc gctacgcgca tcgctaaaga aaagtgtgcg
     4981 taatagcgac tgtgcggcta aagcacttat tgacagacaa agtggtgaac tgattggcat
     5041 ctgtactttt acggcatatt cgctggaaag acaacgcgtt tctggcgtcc ttcagggttc
     5101 acaaccttca gaaattggtg ttgtcagatt agtcatgttg gggggagcgc ggaagtatca
     5161 aaagcggggc tttggtcagg acctactatg tgattttttt gaacatgtaa aaaaaattca
     5221 cccggcatta ccaattaaag gggtttatct tgatgctgac cctgccgcca ttaactttta
     5281 tgctcgtctc ggctttgttc agctttcagc gacaccaaat gcttttggtg ctgtacctat
     5341 gtttttggcg attcagcata ttctcgcggc ttagattatt atcgccaccc gccagggcaa
//
LOCUS       DAJTQY010000178         5339 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.178, whole genome shotgun sequence.
ACCESSION   DAJTQY010000178 DAJTQY010000000
VERSION     DAJTQY010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5339
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.178"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(35..772)
                     /gene="bamD"
                     /locus_tag="OO349_003854"
     CDS             complement(35..772)
                     /gene="bamD"
                     /locus_tag="OO349_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HCR5868753.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRIEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            907..1887
                     /gene="rluD"
                     /locus_tag="OO349_003855"
     CDS             907..1887
                     /gene="rluD"
                     /locus_tag="OO349_003855"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HCR5868754.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRSGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1884..2615
                     /gene="pgeF"
                     /locus_tag="OO349_003856"
     CDS             1884..2615
                     /gene="pgeF"
                     /locus_tag="OO349_003856"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HCR5868755.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2745..5318
                     /gene="clpB"
                     /locus_tag="OO349_003857"
     CDS             2745..5318
                     /gene="clpB"
                     /locus_tag="OO349_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HCR5868756.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKATGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         1311 a         1290 c         1455 g         1283 t
ORIGIN      
        1 ctcaagggct gccgttttgt gtttcaggtt tctgttatgt attgctgctg tttgcggcga
       61 tgattttcgc tactttttca gcttgcgcat tcatctgcat ctgacggtat gcattttcca
      121 tcagcggcag cgcatcacgc gtagcctggg tatccgggta gtcgcgcaac atgccttcta
      181 tgcggttaac gacggcaacc catgcgccac gttctgtata gtactcggcc acggagtatt
      241 catatttcgc cagacgatct ttcaggaata ccagacgttt ggtggcatcg gtagtgtact
      301 gactgttcgg atagccgcgc accagtttgg aaaagtcact aaacgcagct cgtgcatgtt
      361 gaggatcgcg atcgctacgg tcaacgccaa agaacccttg caacgcactg tcatccagcg
      421 ccatattggt caggccacgc atgtacatga cataatcgat attcggatgg gtcgggttaa
      481 ggcgaataaa acgatcgatg gcagcctgcg ctaacggcaa atcggcgttt ttatagtagg
      541 cgtagatgag atccagctgc acctgctgcg aatacggacc aaacggatag cgattatcta
      601 acgcttccag ttgcgttatt gcctgtctcc agttaccgtc ctgcagcttt tgttgtgcag
      661 tcgcgtaaat ttcatttggc ggattatcag gtacttcttc ctttgacccc gagcaacccg
      721 ccaaaaacag gcttagtgtg gcggctgcca ccagatattt catgcgcgtc atgacgtttt
      781 gactttcctc aaaatgtaat acgggagatt ctctgttcct gcacccggtt aagaccagct
      841 acaatagcac actatattaa acggcaaagc cgtaaaaccc caacgataaa cgaagaagca
      901 gtatatatgg cacaacgagt acagctcact gcaacggtgt ccgaaaacca actcggtcaa
      961 cgcttagatc aggctttggc cgaaatgttc ccggattatt cacgttcgcg aataaaagaa
     1021 tggatcctcg accagcgcgt gctggtaaac ggcaaagttt gtgataagcc gaaagaaaaa
     1081 gtattgggtg gcgagcaggt tgccatcaac gctgagattg aagaagaagc gcgttttgaa
     1141 ccgcaggata tccctctgga tatcgtctat gaagatgaag atatcatcgt cattaataaa
     1201 ccgcgcgacc tagtggtaca tcctggcgcg ggtaacccgg atggcacggt actgaatgcg
     1261 ttgcttcatt actatccgcc cattgccgat gtaccacgtt cgggcatcgt ccatcgtctg
     1321 gataaagaca ccactggcct gatggttgtg gcaaaaaccg ttccggctca gacgcgttta
     1381 gtcgaatctt tgcaacggcg tgaaattact cgtgagtatg aagcggtggc gattggtcat
     1441 atgaccgctg gcggtacagt agatgaaccc atcagccgcc acccgaccaa acgtactcac
     1501 atggcggtgc atccgatggg caaaccggcg gtgactcact atcgcatcat ggaacacttc
     1561 cgtgtgcaca cgcgtctgcg gttgcgtctg gaaactggac gtacgcacca gatccgcgtg
     1621 catatggccc atatcactca tccgctggtg ggcgatccgg tttatggtgg ccgtccgcgt
     1681 ccgccaaaag gtgcttcgga agcatttatc tccacgctgc gtaagtttga ccgccaggcg
     1741 cttcatgcaa ccatgctgcg tctttatcac ccgatctccg gcatcgaaat ggaatggcat
     1801 gcgcctattc cacaagatat ggtggagctg attgaggtga tgcgcgccga tttcgaagag
     1861 cataaggatg aagtggactg gttatgagta agctgattgt cccgcagtgg ccgctgccaa
     1921 aaggtgttgc ggcctgtagc tccactcgta tcggcggcgt gagcttgccc ccgtatgact
     1981 cactcaacct cggtgcccat tgtggcgata acccggatca cgttgaggag aatcgcaagc
     2041 gactttttgc tgcgggcaat ttgccttcta aaccggtctg gcttgagcag gtacacggca
     2101 aagatgtgct taagcttact ggcgaacctt atgcctcaaa acgagcagat gcctcttata
     2161 gcaatacgcc tggcacggtt tgcgcagtga tgactgccga ctgcctccct gtgctgtttt
     2221 gcaatcgagc gggaacggaa gtcgccgccg ttcatgctgg ctggcgtgga ctgtgcgcag
     2281 gcgtgctgga agagacggtt tcctgttttg ctgataaacc tgaaaacatt ctcgcctggt
     2341 tagggccggc aattggtcca cgcgcgttcg aagtgggggc ggaggttcgc gaggcgttta
     2401 tggcagtaga cgctaaagca agtgcagctt tcattcagca tggtgataag tatctggcgg
     2461 atatttatca gcttgcccgg cagcgtctgg cgaacgtggg tgttgagcaa attttcggcg
     2521 gcgaccgttg tacatatacg gaaaatgaga ctttcttctc ttatcgtcgc gacaagacca
     2581 ccggtcgtat ggcaagtttc atttggctga tataacctaa agaatcaaga cgatccggta
     2641 cgcgtgattt tcttttcaca ttaatctggt caataacctt gaataattga gggatgacct
     2701 catttaatct ccagtagcaa ctttgatccg ttatgggagg agttatgcgt ctggatcgtc
     2761 ttactaataa attccagctt gctcttgccg atgcccaatc acttgcactc gggcacgaca
     2821 accaattcat cgaaccactt catttaatga gcgccctgct gaatcaggaa gggggttcgg
     2881 ttagtccttt attaacatcc gctggcataa atgctggcca gttgcgcaca gatatcaatc
     2941 aggcattaaa tcgtttaccg caggttgaag gtactggtgg tgatgtccag ccatcacagg
     3001 atctggtgcg cgttcttaat ctttgcgaca agctggcgca aaaacgtggt gataacttta
     3061 tctcgtcaga actgttcgtt ctggcggcac ttgagtctcg cggcacgctg gccgacatcc
     3121 tgaaagcaac aggggcgacc accgccaaca ttactcaagc gattgaacaa atgcgtggag
     3181 gtgaaagcgt gaacgatcaa ggtgctgaag accaacgtca ggctttgaaa aaatatacca
     3241 tcgaccttac cgaacgagcc gaacagggca aactcgatcc ggtgattggt cgtgatgaag
     3301 aaattcgccg taccattcag gtgctgcaac gtcgtactaa aaataacccg gtactgattg
     3361 gtgaacccgg cgtcggtaaa actgccatcg ttgaaggtct ggcgcagcgt attatcaacg
     3421 gcgaagtgcc ggaaggattg aaaggccgcc gcgtactggc gctggatatg ggcgcgctgg
     3481 tggctggggc gaaatatcgc ggtgagtttg aagaacgttt aaaaggcgtg cttaacgatc
     3541 ttgccaaaca ggaaggcaac gtcatcctgt ttatcgacga attacatacc atggtcggcg
     3601 cgggtaaagc cgatggcgca atggacgccg gaaacatgct gaaaccggcg ctggcgcgtg
     3661 gtgaattgca ctgcgtaggt gccacgacgc ttgacgaata tcgccagtac atcgaaaaag
     3721 atgctgcgct ggaacgtcgt ttccagaaag tgtttgttgc cgagccttct gttgaagata
     3781 ccattgcgat tctgcgtggc ctgaaagaac gttacgaatt gcaccaccat gtgcaaatta
     3841 ctgacccggc aattgttgca gcagcgacgt tgtctcatcg ctacattgct gaccgtcagc
     3901 tgccggataa agccatcgac ctgatcgatg aagcagcatc cagcattcgt atgcagattg
     3961 actcaaaacc agaagaactc gaccgactcg atcgtcgtat catccagctc aaactggaac
     4021 aacaggcgtt aatgaaagag tctgatgaag ccagtaaaaa acgcctggat atgctcaacg
     4081 aagaactgag cgacaaagaa cgtcagtact ccgagttaga agaagagtgg aaagcagaga
     4141 aggcatcgct ttctggtacg cagaccatta aagcggaact ggaacaggcg aaaatcgcta
     4201 ttgaacaggc tcgccgtgtg ggggacctgg cgcggatgtc tgaactgcaa tacggcaaaa
     4261 tcccggaact ggaaaagcaa ctggaagccg caacgcagct cgaaggcaaa actatgcgtc
     4321 tgttgcgtaa taaagtgacc gatgccgaaa ttgctgaagt gctggcgcgt tggacgggga
     4381 ttccggtttc tcgcatgatg gaaagcgagc gcgaaaaact gctgcgtatg gagcaagaac
     4441 tgcaccatcg cgtcattggt cagaacgaag cggttgatgc ggtatctaac gctattcgcc
     4501 gtagccgtgc ggggttggcg gatccaaatc gcccgattgg ttcattcctg ttcctcggcc
     4561 caacgggtgt ggggaaaact gagctttgta aggcgctggc gaactttatg tttgatagcg
     4621 acgaggcgat ggtccgtatc gatatgtccg aatttatgga gaaacactcg gtgtctcgtc
     4681 tggttggtgc gcctccggga tatgtcggtt atgaagaagg tggctacctg accgaagctg
     4741 tgcgtcgtcg tccgtattcc gtcatcctgc tggatgaagt ggaaaaagcg catccggatg
     4801 tcttcaatat tctgttgcag gtactggatg atgggcgtct gactgacggg caggggagaa
     4861 cggtcgactt ccgtaatacg gttgtcatta tgacctctaa cctcggttcc gatctgattc
     4921 aggaacgctt cggtgaactg gattatgcgc acatgaaaga gctggtgctc ggtgtggtta
     4981 gccataactt ccgtccggaa ttcattaacc gtattgatga agtggtggtc ttccatccgc
     5041 tgggtgaaca gcacattgcc tcgattgcgc agattcagtt gaaacgtctg tacaaacgtc
     5101 tggaagaacg tggttatgaa atccacattt ctgacgaggc gctgaaactg ctgagcgaga
     5161 acggttacga tccagtctat ggtgcacgtc ctttgaaacg tgctatccag cagcagatcg
     5221 aaaacccgct ggcacagcaa atactgtctg gtgaattggt tccgggtaaa gtgattcgcc
     5281 tggaagttaa tgaagaccgg attgtcgctg tccagtaatg ataaaacgag cccttcggg
//
LOCUS       DAJTQY010000179         5226 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.180, whole genome shotgun sequence.
ACCESSION   DAJTQY010000179 DAJTQY010000000
VERSION     DAJTQY010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5226
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.180"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            146..1072
                     /gene="tauA"
                     /locus_tag="OO349_003858"
     CDS             146..1072
                     /gene="tauA"
                     /locus_tag="OO349_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5868757.1"
                     /translation="MAFIAFQAQAVNVTVAYQTSAEPAKVAQADNTFAKESGASVDWR
                     KFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKLGNSEALVVKKTI
                     SKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGLVEIVNLQPPAIIAAWQRGDIDG
                     AYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHPEVVKAFAKSAID
                     AQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQQQTAELTGPVNK
                     AIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            1085..1852
                     /gene="tauB"
                     /locus_tag="OO349_003859"
     CDS             1085..1852
                     /gene="tauB"
                     /locus_tag="OO349_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HCR5868758.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSDGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLRLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            1849..2676
                     /gene="tauC"
                     /locus_tag="OO349_003860"
     CDS             1849..2676
                     /gene="tauC"
                     /locus_tag="OO349_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HCR5868759.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            2673..3524
                     /gene="tauD"
                     /locus_tag="OO349_003861"
     CDS             2673..3524
                     /gene="tauD"
                     /locus_tag="OO349_003861"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HCR5868760.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSWLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(3630..4604)
                     /gene="hemB"
                     /locus_tag="OO349_003862"
     CDS             complement(3630..4604)
                     /gene="hemB"
                     /locus_tag="OO349_003862"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HCR5868761.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
BASE COUNT         1201 a         1313 c         1454 g         1258 t
ORIGIN      
        1 tcgaagcatt gagcattttt aactcgtttt atcttgaatg caatcggcgc ataacttatt
       61 gaatcccatc aaatcccgtc tttttttacc cattcccggt tatcttgaat aaattaccta
      121 gtcatataac acacttcttg ccgcactggc attcatcgct tttcaggcac aggcggtgaa
      181 cgtcaccgtg gcgtatcaaa cctcagccga accggcgaaa gtggctcagg ccgacaacac
      241 ctttgctaaa gagagcgggg cgagcgtgga ctggcgtaag tttgacagcg gagccagcat
      301 cgtgcgggcg ctggcttctg gcgacgtgca aatcggcaac ctcggttcca gcccattagc
      361 ggttgcagcc agccaacagg tgccgattga agtcttcttg ctggcgtcaa aactgggtaa
      421 ctccgaagcg ctggtggtaa agaaaactat cagcaaaccc gaagatttga ttggtaaacg
      481 cattgccgta ccgtttatct ccaccaccca ctacagcctg ctggcggcgc tgaaacactg
      541 ggggattaaa ccagggctag tggagattgt gaacctgcag ccgcccgcga ttatcgccgc
      601 atggcagcgg ggtgatattg atggtgctta tgtctgggca ccggcggtta acgccctgga
      661 aaaagacggc aaggtgctga ccgattctga acaggtcggg cagtggggtg cgccaacgct
      721 ggacgtctgg gtggtgcgca aagattttgc cgagaaacat cctgaggtcg tgaaagcgtt
      781 cgccaaaagc gccatcgatg cacagcaacc gtacattgct aaccctgacg cgtggctgaa
      841 acagccggaa aacatcagca aactggcgcg tttaagcggc gtgcctgaag gtgacgttcc
      901 ggggctggtg aagggaaata cctatctgac gccgcagcaa caaacggcag aactgaccgg
      961 accggtgaac aaagcaatca tcgacaccgc gcagtttttg aaagagcagg gcaaggtccc
     1021 ggctgtagcg aatgattaca gccagtacgt tacctcgcgc ttcgtgcaat aaaaggaggc
     1081 gcagatgctg caaatctctc atctctatgc cgattatggc ggcaaaccgg cactggaaga
     1141 tatcaacctg acgctggaaa gcggcgagct actggtggtg ctggggccgt ccggctgcgg
     1201 taaaaccacc ctgctgaatc tgattgccgg ttttgtgcct tatcagcatg gcagcattca
     1261 actggcgggt aagcgtattg agggaccggg agcagagcgt ggcgtagttt ttcagaatga
     1321 agggctgcta ccgtggcgca atgtacagga caacgtggcg ttcggcctgc aattggcagg
     1381 tatagagaaa atgcagcgac tggaaatcgc gcaccagatg ctgaaaaaag tggggctgga
     1441 aggcgcagaa aaacgctata tctggcagct ttccgatggt caacgtcagc gggtggggat
     1501 tgcccgcgcg ctggcggcga atccccagct gttattactc gacgaaccgt ttggtgcgct
     1561 ggacgccttc acccgcgacc agatgcaaac tctgctgctg aaactctggc aggagacggg
     1621 caagcaggtg ctgttgatta cccacgatat agaagaagcg gtgtttatgg cgactgaact
     1681 ggttctgctt tcatccggcc ctggccgtgt gctggagcgg ctgcggctca actttgctcg
     1741 ccgctttgtt gcgggagagt cgagccgcag catcaagtcc gatccacaat tcatcgccat
     1801 gcgcgaatat gttttaagcc gcgtatttga gcaacgggag gcgttctcat gagtgtgctc
     1861 attaatgaaa aactgcattc gcggcggctg aaatggcgct ggccgctctc gcgtcaggtg
     1921 accttaagca ttggcacgtt agcggtttta ctcactgtat ggtggacggt ggcggcgctg
     1981 caactgatta gcccgctgtt tttgccgccg ccgcaacaag tactggcaaa actgctcacc
     2041 attgccggac cgcagggatt tatggacgcc acgctgtggc agcatctggc agccagcctg
     2101 acgcgcattg tgctggcgct actcgcggcg gtggtgatcg gtattccggt cgggatcgcc
     2161 atggggctaa gcccgacggt gcgcggcatt ctggatccgg taatcgaact ttatcgtccg
     2221 gtgccaccgc tggcttattt gccgctgatg gtgatctggt ttggtattgg tgaaacctcg
     2281 aagatcttac tgatctattt agcgattttt gcgccggtgg cgatgtcggc gctggcaggg
     2341 gtgaaaagcg cgcagcaggt tcgcattcgt gccgcccagt cgctgggtgc cagccgtgcg
     2401 caggtgctgt ggtttgtcat tttgcccggc gcgctgccgg aaatcctcac cggattacgc
     2461 attggtctgg gtgtgggctg gtctacgctg gtagcggcgg agctgattgc cgcgacacgc
     2521 ggtttaggat ttatggttca gtcagcgggt gaatttctcg cgactgacgt ggtactggcg
     2581 gggatcgcgg tgattgcgat tatcgccttt cttttagaac tgggtctgcg cgcgttacag
     2641 cgccgtctga cgccctggca tggagaagta caatgagtga acgtctgagc attaccccgc
     2701 tggggccgta tatcggcgca caaatttcgg gtgccgacct gacgcgcccg ttaagcgata
     2761 atcagtttga acagctttac catgcggtgc tgcgccatca ggtggtattt ctgcgcgatc
     2821 aagctattac gccgcagcag caacgcgcgc tggcccagcg ttttggcgaa ctgcatattc
     2881 accctgttta ccctcatgcc gaaggggttg acgagatcat cgtgctggat acccataacg
     2941 ataatccgcc ggataacgac aactggcata ccgatgtgac atttattgaa acgccacccg
     3001 caggggcgat tctggcagct aaagagttac cttcgaccgg cggtgatacg ctctggacca
     3061 gcggtattgc ggcctatgag gcgctctctg ttcccttccg ccagctgctg agctggctgc
     3121 gtgcggagca tgatttccgt aaatcattcc cggaatacaa ataccgcaaa accgaggagg
     3181 aacatcaacg ctggcgcgag gcggtcgcga aaaatcctcc gttgctacat ccggtggtgc
     3241 gaacgcatcc ggtgagcggt aaacaggcgc tgtttgtgaa tgaaggcttt actacgcgaa
     3301 ttgtcgatgt cagcgagaaa gagagcgaag ccttgttagg ctttttattc gcccatatca
     3361 ctaaaccgga gtttcaggtg cgttggcgct ggcaaccgaa tgatattgcg atttgggata
     3421 accgcgtgac tcagcactat gccaatgccg attacctgcc acagcggcgg attatgcatc
     3481 gggcgacgat ccttggggat aaaccgtttt atcgggcggg gtgatacgag tgcggacggt
     3541 tccctcgccc ccttggggag agggttaggg tgagggggcg ttcactgtac tttcaacagg
     3601 ttaactcccc ctttctgaga ggaaaaaaat taacgcagaa tcttcttctc agccaaatcc
     3661 agcgcaaagt agctgaaaat cagatccgca cccgcacgct taattgaacc taagctttcg
     3721 agcacgactt tctcttcatc tatagcaccc gccagcgcgg cgaacttaat catcgcgtac
     3781 tcaccgctca cctgatacgc gccaatcggc aattcagtac gttcacgcag ctcacgcacg
     3841 atgtcgaggt acgctccagc aggtttaacc atcaggcagt ctgcgccctg ggcttcatcc
     3901 agcagtgact cacgaatcgc ctcgcgacgg ttcattgggt tcatctgata gcttttgcgg
     3961 tcgcctttta atgcgcttcc tgcagcttca cggaacgggc cataaaagga agaggcgaac
     4021 ttggtcgaat acgacataat cgccgtatct ttaaagcctg cagcgtccag cgcctgacga
     4081 atcgcctgta cctggccgtc catcgcggcg gaaggggcga tgaagtctgc gcctgcagca
     4141 gctgcaacca cggcttgctt gcctaaattt tccagagtcg cgtcgttgtc gacgccatgc
     4201 tcgcacagca caccgcagtg accgtgagaa gtgtattcac agaaacaagt gtctgacatg
     4261 acgatcattt ctggcacagt ctgcttgcag atgcgcgaca tacgcgccac cagtccatct
     4321 tcccgccagg cgtcgctgcc ggtttcatcg gtatggtgag agatgccaaa agtcattacg
     4381 gaacggatac cggcgttggc gatgcgttca atttcgcgtg ccagatgttt ctctggaatg
     4441 cgcatcacgc ctggcatggc ttcaacggct ttgtagtcgt caatttcttc ttcaacaaag
     4501 atcggcaaca ccaggtcgtt aaggctaagt gttgtctctt caaacatagc gcgcagcgca
     4561 ggagatttgc gcaggcgacg agggcgttgg attaagtctg tcatggtctg cctgatgttt
     4621 gtggaattaa ggtgcacagt atacctgaag cggggcagga gtgttttacg aaagttgtcg
     4681 cgatgttgac aagaagagaa tgaaagagag accaggacat ctttgtaata gaaatagcaa
     4741 taatttcata ttcactgaaa atatttttaa tctttattta tagctcttgg ttattatttt
     4801 ttggcgtttg gttgtgctgc tataaattga ataattcaaa ctttgttgca tattgataag
     4861 atatttatct ggtttgtgtt tttgatattt ataacgcgta cgcatcaaaa ataacattat
     4921 ctgcaacata ctgaaatatc gtccttttat caatttgatg cgtgtttttc tccataaatt
     4981 atatatgtcc acattcggac ttaggggaaa gaataattga accattcgtc tgtaacgcag
     5041 cataatcgtt agcgcgaaac ataatatgtt ttctatgcat tgataattga tggatcaact
     5101 tattacgtcc ctgaggaggg atgacaaatg cactcctgga aaaagaaact tgtagtatca
     5161 caattagcat tggcttgcac tctggctatc acctctcagg ctaatgcagc gaccaacgat
     5221 atttct
//
LOCUS       DAJTQY010000180         5163 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.181, whole genome shotgun sequence.
ACCESSION   DAJTQY010000180 DAJTQY010000000
VERSION     DAJTQY010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5163
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.181"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            <1..221
                     /locus_tag="OO349_003863"
     CDS             <1..221
                     /locus_tag="OO349_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5868762.1"
                     /translation="LYRMFADKGLVVAQYIRNRRLDFCADAIRHAADDEKLAGIGFHW
                     GFSDQSHFSTVFKQRFGMTPGEYRRKFR"
     gene            complement(393..722)
                     /locus_tag="OO349_003864"
     CDS             complement(393..722)
                     /locus_tag="OO349_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HCR5868763.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTEINAERKASYQQRAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(727..912)
                     /locus_tag="OO349_003865"
     CDS             complement(727..912)
                     /locus_tag="OO349_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HCR5868764.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(909..3548)
                     /locus_tag="OO349_003866"
     CDS             complement(909..3548)
                     /locus_tag="OO349_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HCR5868765.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLADIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWLYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLNVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRNPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            3756..4745
                     /gene="ldhA"
                     /locus_tag="OO349_003867"
     CDS             3756..4745
                     /gene="ldhA"
                     /locus_tag="OO349_003867"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCR5868766.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSHPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            4856..>5163
                     /gene="hslJ"
                     /locus_tag="OO349_003868"
     CDS             4856..>5163
                     /gene="hslJ"
                     /locus_tag="OO349_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HCR5868767.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     "
BASE COUNT         1305 a         1322 c         1205 g         1331 t
ORIGIN      
        1 gtttgtaccg aatgtttgcc gataaaggtt tggtagtcgc gcaatatatt cgtaaccgtc
       61 gtctcgattt ttgtgcagat gcgattcgcc atgccgcaga tgatgaaaaa ctggcaggca
      121 tcggctttca ttggggattt tctgaccaga gtcatttttc aacggtattt aagcaacgct
      181 ttgggatgac gccaggcgag tatcgacgta aattccgcta actaattggc gttgcatgta
      241 ctgcaacgcc aattagttaa aacagatatt tgactatgca tttttcaggg cgaaaggcaa
      301 tttgacagga gtttttgaat attcaccttg tttcaggaaa atagatcaat cgctttctcc
      361 tgtagcgtta tcccgatatt gcggttttgc tattaaaact ttcgcaccca tttaccgttt
      421 atcccttgta catattcccc cggtttggcc cgagctacga gcttttgccc ggcgagtttc
      481 gcgatatcat ctacagacac attattttgc tttgcccgtt gttggtaact cgctttacgt
      541 tcggcattaa tttcggttac taatttctct gtttcagcat ccgttttcag cgcaaccaga
      601 taaccataaa atgtttcacc tacccgcccc tgagttctgg cttcatccag agtcaatgcc
      661 attacattgc tgcttaccag cccaacaaga aacgcacaaa gaagtaatgt tttcttcatc
      721 atcgcctcag aaaagatcgc tacgagtttc aagcagctct tcgacatctt tgtctgcctt
      781 gatgatgatc tcatgctcaa ttttaacgtt catattgata gtgatcggtt ccttaggtgc
      841 agcgacttca atgcgaggcg tacagccaac cagcataaaa gatgacgtca acgcagccag
      901 taaaattttc attgtttttc ctcacactct tttccttgcg gacagtcatt tcccggcaga
      961 cgtgcgttct gctccaacca tgcctggaga ttatcgccaa agcgtaaact gcgccacagc
     1021 gtaaaaatat tctcttcatg atggtaatta aggtttaccg taccgctttt accatcaacg
     1081 cgactggtac ctgtaatgtt ggcctgcatg gttaataaac cgagattatc taagttaatt
     1141 tttgtcgatg accggctaat ttccatatag cgcaaccagt taattgctga acccgcagtc
     1201 atattgtctt tcaccaccgc atctgcggta tctttatcca gccgcaaggt cattggcccg
     1261 ctatttgcca gccaaccatc tttcactatc catttttcgt tgtttagcca taacggcaat
     1321 gcaccactaa atgccccgga catggcgaat tgtttcggat tgacggcgct aaccagttcg
     1381 ctggatgaga gattattcag ccgcaacagc gccgggtcat gttgcggcat acgtaattgt
     1441 ttcatcagca cgttaccgcc taacacatcg acgctaacat cagtcaacag caagggttct
     1501 tcttcagtcc acggataacg cccttgcaaa tcagccgtaa tatttttcgc tgtcaccaga
     1561 ttaatcactt cggcaattcg caacgtaacg gggccgcgag tccccagatg ccaggctcca
     1621 tcggcaaaac ggaaaggcag gacaaaatcg acaccgttaa cctgattatc tggcatccag
     1681 gcactaccgc ctttcaacac gccgtgccct cccgcgcgga atccttgttc aggcgcagca
     1741 gaaaatgcaa cctgagcata cagttcacca tcgcgtaagt tcatcttcca gtcgggtggc
     1801 accagcggct ggaatacagt cagtgattgt tttggccacc aggcgttacc gcgcagacga
     1861 ataccgtccc agcgaccatt tacccgaacc gggccaatct caccagcatg taaatcgcct
     1921 ttaaagagga aataggtcgg attgcgccca tcgacgctaa attttaaggt tgatggcggt
     1981 aacacactgc cgccagtgaa cagcgtttgc ccggcgtcca gtgacagcgc gccgctgaag
     2041 gagggatgct gtgcgtcacg tacccagacg acgggcttgt cgagaattaa tcgcggcttt
     2101 tctaccgtca tcgtaccgta ttgtaactgg tcgaaaccgg tggagagatc ggtcagcgta
     2161 atcgtgctgt catgccactc accttttcct gcgacatccc aggtggcatt catcggtgta
     2221 aaactccctt ttccccagta gcgccactgc cagcggccag cgtcagggag aaaatcattc
     2281 gccagcccat ccatatgcag cacgaaatcg cccagttcat tttcatgcgc ctgcaaaatg
     2341 gcctgcaaac gtccgtcaac accacgttgg gtgactttta cacccgctaa aggccagcgg
     2401 atttcatcga tgtccagcga atcgatgact cttccctttg aacgaagtaa cgcgcctggc
     2461 tcaaaggtca gcgttgggtc agacagactt ccacttagct gcgcaggtaa acgggcatat
     2521 aaaatgaggt ccgcctgttt cgcttcaccg gtcagctgca gaggcagttg actgttatcc
     2581 atgcttaatt ttcctgggcc aaaattaagc accgcgttgc ccttacccgc ttgcccctgg
     2641 gtcagcacat tcagtcgtcc gctgaccaga gcgttctcaa gccctgcctg ccagttgtcg
     2701 actttgacac ccagtcggcc actcaaagga aaacctgcat acagccagct ccagcgacca
     2761 tcgcttacgg tcaattgttg acgagtaatt tgccacggca aatcgagcaa cggatcgccg
     2821 ttatcccgtg ccagcacgat caattgcccg ctattttcct gccagtccag ctcggcatcc
     2881 accagtgacg gttcctgcgg caagtttaac gtcgcagtag catgcccact tacaggaagt
     2941 ccatccggca cgagcggcat agtaaattcc cccaccagtt taaccggcgg ctgattttca
     3001 aacgcgacga catccagttc gctgactgta agttgttgcc ctttcagctg gccttgaaat
     3061 ttaacttttt cgccctgata acgcagttgc tggatatcag aggttaatgc gagagagagt
     3121 tttccctgcc attcctgcca gggagaaaaa atcagtttat cgatattgat ccaggtgtta
     3181 ggcagcatgg actgccactg cgcgagggtt tttggagcag ctggcgattg ctccgtctgc
     3241 ggcaatttcg ccagacaagc agaatcaagt tctaccgtgc cgacgttcaa taaccagcgg
     3301 ctgggatgtg aaaggctggc gttggtgata tgcgcaagct gacaatctcc caccagataa
     3361 cggagatcgg gaatgattaa acctttacgc gtaatgcgtg ggctttcatc taatgcaata
     3421 cgtgtcccga gcggtagcca gatgtccgcc agcgtgggaa cccacagccc gagcgtcatc
     3481 agcagcgtca acggcacaag aataatcagt aataacagcg cgagaacggc tttatattta
     3541 cccagcatgg gtagttaata tcctgattta gcgaaaaatt aagcattcaa tacgggtatt
     3601 gtggcatgtt taaccgttca gttgaaggtt gcgcctacac taagcatagt tgttgatgaa
     3661 tttttcaata tcgccatggc tttcaattat atttgaaatt ttgtaaaata tttttagtag
     3721 cttaaatgtg attcaacatc actggagaaa gtcttatgaa actcgccgtt tatagcacaa
     3781 aacagtacga caagaagtac ctgcaacagg tgaacgagtc ctttggcttt gagctggaat
     3841 tttttgactt tctgctgacg gaaaaaaccg ctaaaactgc caatggctgc gaagcggtat
     3901 gtattttcgt aaacgatgac ggcagccacc cggtgctgga agagctgaaa aagcacggcg
     3961 ttaaatatat cgccctgcgc tgtgccggtt tcaataacgt cgaccttgat gcggcaaaag
     4021 aactggggct gaaagtagtc cgtgttccag cctatgatcc agaggccgtt gctgaacacg
     4081 ccatcggtat gatgatgacg ctgaaccgcc gtattcaccg cgcgtatcag cgtacccgtg
     4141 acgctaactt ctctctggaa ggtctgaccg gctttactat gtatggcaaa acggcaggcg
     4201 ttatcggtac cggtaaaatc ggtgtggcga tgctgcgcat tctgaaaggt tttggtatgc
     4261 gtctgctggc gttcgatccg tatccaagtg cagcggcgct ggaactcggt gtggagtatg
     4321 tcgatctgcc aaccctgttc tctgaatcag acgttatctc tctgcactgc ccgctgacac
     4381 cggaaaacta ccatctgttg aacgaagccg ccttcgatca gatgaaaaat ggcgtgatga
     4441 tcgtcaatac cagtcgcggt gcattgattg attctcaggc ggcaattgaa gcgctgaaaa
     4501 atcagaaaat tggttcgttg ggtatggacg tctatgagaa cgaacgcgat ctgttctttg
     4561 aagataaatc caacgacgtg atccaggatg acgtattccg tcgcctgtct gcctgccaca
     4621 acgtgctgtt taccgggcac caggcattcc tgacagcaga agctctgacc agtatttctc
     4681 agactacgct gcaaaactta agcaatctgg aaaaaggtga aacctgcccg aacgaactgg
     4741 tttaatcttg cagctcccct gcaatccagg ggagctgatt cagataatcc ccaatgacct
     4801 ttcattctct attcttaaaa tagtcctgag tcagaaactg taattgagaa ccacaatgaa
     4861 gaaagtagcc gcgtttgttg cgctaagcct gctgatggcg ggatgtgtaa gtaatgacaa
     4921 aattgctgtt acgccagaac agctacagca tcatcgcttt gtgctggaaa gcgtaaacgg
     4981 taagcccgtg accagcgata aaaatccgcc agaaatcagc tttggtgaaa aaatgatgat
     5041 ttccggcagc atgtgtaacc gctttagcgg tgaaggcaaa ctgtctaatg gtgaactgac
     5101 agccaaaggg ctggcaatga cccgtatgat gtgcgctaac ccgcagctta atgaactcga
     5161 taa
//
LOCUS       DAJTQY010000181         5156 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.182, whole genome shotgun sequence.
ACCESSION   DAJTQY010000181 DAJTQY010000000
VERSION     DAJTQY010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5156
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.182"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            188..445
                     /locus_tag="OO349_003869"
                     /pseudo
     CDS             188..445
                     /locus_tag="OO349_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
     gene            complement(495..1445)
                     /gene="uspE"
                     /locus_tag="OO349_003870"
     CDS             complement(495..1445)
                     /gene="uspE"
                     /locus_tag="OO349_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HCR5868768.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTTMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1597..2349)
                     /gene="fnr"
                     /locus_tag="OO349_003871"
     CDS             complement(1597..2349)
                     /gene="fnr"
                     /locus_tag="OO349_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HCR5868769.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(2544..3059)
                     /gene="ogt"
                     /locus_tag="OO349_003872"
     CDS             complement(2544..3059)
                     /gene="ogt"
                     /locus_tag="OO349_003872"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HCR5868770.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(3070..3213)
                     /locus_tag="OO349_003873"
                     /pseudo
     CDS             complement(3070..3213)
                     /locus_tag="OO349_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
     gene            complement(3603..4418)
                     /locus_tag="OO349_003874"
     CDS             complement(3603..4418)
                     /locus_tag="OO349_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707689.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5868771.1"
                     /translation="MTITLQPKASPGHHIIGLDSEHLNGGTVPWHKHLYAQLLYPAEG
                     VVRVWAGESVWLVHASSALWLPPQMPHKFVATGNVLLKTVLVSEAESETLGTVCFMTG
                     ISPLLRELLIAINQLPPSQSTTDKQQLRFSALETLILQEIKMGVKMSLELPWPNDERL
                     QQLCENLLNNQGYLPTLDNLADKINVSSRTLMRLFVKETGLTFRHWVQQMHVISAVTL
                     LDDGYSLTKIAHRLGYASAESFGNMFKRRTGYSPGKFTRRLTMHNYAITRQMI"
     gene            4515..>5156
                     /locus_tag="OO349_003875"
     CDS             4515..>5156
                     /locus_tag="OO349_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR5868772.1"
                     /translation="MIISKKQLIGVVAIGILLTGVVFFIWRVSKGRFIQTTDDAYIGG
                     NITTVASKVSGYISAIEVRDNQSVKKGDIILRLDDRDYRANVARLEAKIKSSKANLEG
                     IQATITMQQSIIQSASETWQAVKHEEQKRLRDTERYEKLAQSAAISQQIIDNARFDYQ
                     QVAAKERKAANDFLVEKQRLAVLSAQEENVRASIEEVQAALTQALLDLEYTLVH"
BASE COUNT         1376 a         1175 c         1226 g         1379 t
ORIGIN      
        1 tattttaccg cgacaattca ttcagaacat caatagtcag ggaaggaagt agcaacatta
       61 gctaactaat caatgcgttg attgtaaatc cagctaagag gtgaggtttt cagagcagac
      121 aacggtgaaa cgtcatggta ttgttacgtt taggtaacaa gaaatttgtc tgcacaagga
      181 ttacatcatg attatggcga aactgaagtc agcgaaaggg aagaaatttc tctttggttt
      241 gttggcggtt ttcattattg cggcgtcggt tgtgactcgc gcgaccatcg gcggcgttat
      301 agaacagtac aatattccgc tgtctgagtg gacgacatca atgtatgtga tttagtcatc
      361 gatgattttt gtttatagcc tggtctttac tgtgttgctg gcaatcccgt tgggaattta
      421 tttccttggc ggcgaagagc agtaagtaaa aaataggccc gataactcgg gccttgtcag
      481 ttattgaaga gtcgttaatc gtcttcttcg tcatccagtt caacgggtgt ctgatactgg
      541 tcaggtttaa tgaccagcag gtcgcagcga agatgatcaa tcacctgttc cgccgtgttg
      601 ccgaggaatg ctgctgaaat accggtgcgt ccgaccgtgc ccagaaccac aattcccgcc
      661 tgtaaatgct ccgccaaatc aggaatcacc tcttcgggca gacctttttc tacgtgcgtc
      721 atgttttcat taatgccgaa tttctgccgc agggctttca ttgccagcaa atgttgccca
      781 cgaatggcat cgttataaac gctcgggtca aattccggca gttcaatcgc gatattaatt
      841 ggcgttaccg gataagcgcc aaccagatga acttcggtat ggttgacttg ttctgccagt
      901 tcgatcgtct ctttgaccag tttttcattg agcgcattat gatacggctc ttcactggcg
      961 agattcaccg ccaccagcgc cttgcctcct tccggccacg gctggtcttt caccatccac
     1021 accgggcttg ggcatttgcg taacagatgc cagtccgttg gcgtaaaaat caccgcttcc
     1081 agacggtcat gttggtgcgc catttttagc accaaatcgt gtccgccgct gatcacttcc
     1141 tgaatgatgg cttcgaaagg acggttatgc cagaccactt taatttcaat gggaacgcca
     1201 gcattgagat aatattttgc ctgctcgtgg atccaggctg tacgctggct gatgacgccc
     1261 tgacgcatag tggtacgttc gtccggggag agcagggtgg tcatttcgta tgagaagtca
     1321 tagatcggca aaaaggcttt aattttgcca ccaatccgtt gatgtaaata aacagctcgc
     1381 cgcaatgctg gttggtcgtc ctggttagga tcgataacaa cgagcatgtt ctgatacata
     1441 gccatacagg gtctccttac aacaactgtc aacgcagttt gtaattaaaa gattaaccca
     1501 tatctggtga atgaaacagt gatgaacctt ctgccagatc aataaatcag aaaaatttaa
     1561 tgatatgaca gaaggatagt gagttatgcg gaaaaatcag gcaacgttac gcgtatgacc
     1621 agcaagctgg gccagcgcat cgttattttc gatggtgatg tatttacctt tgactgccag
     1681 cataccgctt ttctggaagc gacccagcag acggctgatg gtttctaccg tcaggcccag
     1741 atagttacca atatcgccac gagtcatcgt caggcggaat tcacgagggg agaagccgcg
     1801 ttgggcaaaa cgacgggaca ggttgtagat gaatgcagcc agacgttcct cggcattttt
     1861 cttcgacaac agcaagatca tgtcctgatc gcctttgatt tcaccgctca tcagacgcat
     1921 catctgctga cgcagattcg gcattttacc ggacaaatcg tccagcgttt cgaacggaat
     1981 ttcacatacc atcgaggttt ccagcgcctg agcgaagctc gggtgatggc cgctgccgat
     2041 ggcgtcaaat cccactaggt cgcctgctaa atggaaacca gtgatttgct cgtcgccttg
     2101 ctcagtgatt gtataacttt taatcgtacc ggagcggatg gcataaagcg atttaagttc
     2161 atcaccagcc ttaaacagcg tctggccttt ctgaataggc ttcttccgct caatgatatt
     2221 atcaagctga tcaagctcat gttcgttgag tgtgaacggg atgcaaagct ggctgatgct
     2281 gcaatcctgg caatggatag cacaaccgcc agactgaatg cgccgtataa ttcgcttttc
     2341 cgggatcata ggtctgctca agccgtaatt gatatttgtc aattttaaca tctttttagg
     2401 gagcaagtaa gtctaagcaa accttaacag cagagaattc cgatattaga tgtaaatata
     2461 tgtctatcta tttgaaactc cttaagttgt caagggtaac tttacataaa agtgtgaaca
     2521 agctggcaca aattgtttaa tgtttacagc aaaagataac cttcgtggcg caataaccac
     2581 tcttttcgct gaactccgcc tgcatatccg gtcatggtgc cgtttcggcc aataacccga
     2641 tggcaaggta cgacgatgct gatgggattc gatccgtttg ccgcaccaac ggcacgcgcc
     2701 gcgccagggc ggcccaattg ttcagccagt tggccgtaat gcattacctg cccgcagggg
     2761 atagtgcgta gtgttttcca gacttcgcgc tgaaatggcg tcccccccgt ggcagtggga
     2821 agcgtatcaa taatgctaag attaccggca aaatattcac gaagcttgtc gcttaaacca
     2881 cctgggttgg tggcagaaat gcgctcatag ccttctttgc gataatggat gtccagcagc
     2941 tgcaccatgc gttcgctgta ctcttcccat tcaaccgccc gcaggcgaaa ttgctcatcg
     3001 caaatcaccc acagtggacc cagtggcgtg gcaatttttt cttcaagtaa tctcagcatc
     3061 cgttctctct taagacaaac gtgggtaaat acccggacct atcggcagac cgacaagata
     3121 ccacgccagc aacatcagca gccataccac caaaaagata agtggatagg gcaagactaa
     3181 cgaatagtaa gtacccagtt tcgcgtctgg tttgtcgcag tctgatagca taaagcgatt
     3241 ttttaatttt tctgcctgaa gaaatgaggg caagccatca catggatcga gggtgggata
     3301 attccagtca gtgaaatttt accgtaggct atctgttatt tgattctgtg gcgtgatagc
     3361 cgccagacac cactgaactc gtcactcatt ccccaatccc ggcgagaatg tggttattta
     3421 cagagctaaa aaatgaataa aaaaatagag cctgtcgaca tccgccagac tctacagtac
     3481 acacagcagt gcatcctcgt cttaaatccg gcctggggtc ctggtgaccg gtgacagtgt
     3541 tataacggtt ttacccatcc gttgccaatt ttcttcagtg acagatttca cgaaaatata
     3601 agttaaatca tttgtcttgt gattgcataa ttatgcatcg ttaatcgacg agtaaattta
     3661 ccgggcgagt agcccgttcg acgcttaaac atattgccga acgattcagc tgaggcatag
     3721 cccagacgat gggcaatttt ggtcaacgaa tatccatcat caagaagcgt cactgcagaa
     3781 ataacgtgca tttgctgaac ccagtggcga aatgttaatc ccgtctcttt aacaaataag
     3841 cgcatgagtg tacggctact gacgttaatt ttatccgcta gattatccag tgttggcaga
     3901 tacccttggt tgtttagcaa attctcacaa agctgctgta gccgctcatc atttggccaa
     3961 gggagttcaa gtgacatttt cacccccatt tttatttcct gaagtatcag tgtctcgagg
     4021 gctgaaaaac gtaactgttg tttatctgtg gtactttgcg acggtggaag ttggttaatt
     4081 gcaattagta attcccttaa caggggtgaa atacccgtca tgaagcaaac tgttcccagc
     4141 gtttcacttt ccgcttctga aaccagtacg gtttttaaaa gtacgttgcc tgtcgcaaca
     4201 aacttatgcg gcatttgtgg gggcagccaa agcgcgctgc tggcatggac tagccagacg
     4261 ctttctccag cccatacacg aacgactcct tcagccgggt aaagcaactg ggcatagaga
     4321 tgtttatgcc agggaactgt acctccattc agatgttccg aatccagtcc gataatgtga
     4381 tgccctggtg atgctttggg ttgcagtgta attgtcattt acagtaggta ttttgtcaga
     4441 aatcatcaaa taagccatgt taaattttaa atatgacgta tgcaagtttc cggatatata
     4501 cggtgtaaag agtgatgata atttctaaaa aacagcttat tggggtggta gcgatcggta
     4561 ttcttttgac tggcgttgtc ttttttatct ggagggtaag caaaggaaga tttatacaga
     4621 cgacggatga tgcttacatt ggtggtaata tcaccacggt ggcaagtaaa gtttctggtt
     4681 atatttctgc tatagaggtg cgcgataatc agagcgtaaa aaaaggcgat attatcctgc
     4741 gtctcgatga tcgggattat cgtgcaaatg tcgccaggct tgaggcaaaa ataaaaagta
     4801 gcaaagccaa tctcgaaggc attcaagcca ctattacgat gcagcagtcg ataattcaaa
     4861 gtgccagtga aacatggcag gcagtaaaac atgaggagca aaagcgcctg cgagatactg
     4921 aacgttatga aaaactcgca caatcagcgg caatatcgca acaaattatt gataacgcca
     4981 ggtttgacta ccagcaggtc gcagcaaagg agcgcaaagc cgcgaacgat ttcctggtgg
     5041 agaagcaacg gctggcagtt ttatctgctc aggaagaaaa cgtacgtgcg agtatcgaag
     5101 aagttcaggc cgctttgact caggcactac tggatctgga atataccctt gttcac
//
LOCUS       DAJTQY010000182         5023 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.183, whole genome shotgun sequence.
ACCESSION   DAJTQY010000182 DAJTQY010000000
VERSION     DAJTQY010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5023
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.183"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            47..121
                     /gene="tnaC"
                     /locus_tag="OO349_003876"
     CDS             47..121
                     /gene="tnaC"
                     /locus_tag="OO349_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HCR5868773.1"
                     /translation="MNILHVCVTSKWFNIDNKIVDHRP"
     gene            342..1757
                     /gene="tnaA"
                     /locus_tag="OO349_003877"
     CDS             342..1757
                     /gene="tnaA"
                     /locus_tag="OO349_003877"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HCR5868774.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYRKEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            1848..3095
                     /gene="tnaB"
                     /locus_tag="OO349_003878"
     CDS             1848..3095
                     /gene="tnaB"
                     /locus_tag="OO349_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HCR5868775.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNIDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            3227..4402
                     /gene="mdtL"
                     /locus_tag="OO349_003879"
     CDS             3227..4402
                     /gene="mdtL"
                     /locus_tag="OO349_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HCR5868776.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTFSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            4377..>5023
                     /gene="yidZ"
                     /locus_tag="OO349_003880"
     CDS             4377..>5023
                     /gene="yidZ"
                     /locus_tag="OO349_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HCR5868777.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTQLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDT"
BASE COUNT         1145 a         1209 c         1255 g         1414 t
ORIGIN      
        1 ctgagtgtaa taatgtagcg gcgtgtcttt cgaggataag tgcattatga atatcttaca
       61 tgtatgtgtg acctcaaaat ggttcaatat cgacaataag attgtcgatc accgccctta
      121 atttaccctt ctgtagccat caccagagcc aaaccgatta gattcaatgt gatcgattag
      181 tttgctatat cttaattttg ccttttgcaa aggccatctc tcgtttattt acttatttta
      241 gtaaatgatg gtgcttgtat atatacatgg tgaattaatg gatattgcag atgtaatatt
      301 cacagggatc actgtaatta aaataaatga aggattatgt aatggaaaac tttaaacatc
      361 tccctgaacc gttccgcatt cgtgttattg agccagtaaa acgtactact cgcgcttatc
      421 gtaaagaagc aattattaaa tccggtatga acccgttcct gctggatagc gaagatgtgt
      481 ttatcgattt actgaccgac agcggcaccg gggcggtaac ccaaagtatg caggcagcga
      541 tgatgcgcgg cgacgaagcc tacagcggca gccgcagcta ctatgcgtta gccgagtcag
      601 tgaaaaatat ctttggttat caatatacta ttccgactca ccagggccgt ggcgcagagc
      661 aaatctatat tccggtactg attaaaaaac gcgagcagga aaaaggcctg gatcgcagca
      721 aaatggtggc attctctaac tatttctttg ataccacgca gggccatagc cagattaacg
      781 gctgtaccgt gcgtaacgtc tatatcaaag aagccttcga tacgggcgtg cgttacgact
      841 ttaaaggcaa ctttgacctc gaaggattag aacgcggtat tgaagaagtt ggcccgaata
      901 acgtgccgta tatcgttgca accatcacca gtaactctgc aggtggtcag ccggtttcac
      961 tggcaaactt aaaagcgatg tacagcatcg cgaagaaata cgatattccg gtggtaatgg
     1021 actccgcacg ctttgctgaa aacgcctatt tcatcaagca gcgtgaagca gaatacaaag
     1081 actggaccat cgagcagatc acccgcgaaa cctacaaata tgccgatatg ctggcgatgt
     1141 ccgccaagaa agatgcgatg gtgccgatgg gcggcttgct gtgcatgaaa gacgacagct
     1201 tctttgatgt gtacaccgag tgcagaaccc tttgcgtggt gcaggaaggc ttcccgacat
     1261 atggcggcct ggaaggcggc gcgatggagc gtctggcggt aggtctgtat gacggcatga
     1321 atctcgactg gctggcttat cgtatcgcgc aggtacagta tctggtcgat ggtctggaag
     1381 agattggcgt tgtctgccag caggcgggcg gtcacgcggc attcgttgat gccggtaaac
     1441 tgctgccgca tatcccggca gaccagttcc cggcacaggc gctggcgtgc gagctgtata
     1501 aagtcgccgg tatccgtgcg gtagaaattg gctctttcct gttaggccgc gatccgaaaa
     1561 ccggtaaaca actgccatgc ccggctgaac tgctgcgttt aaccattccg cgcgcaacat
     1621 atactcaaac acatatggac ttcattattg aagcctttaa acatgtaaaa gagaacgcgg
     1681 cgaatattaa aggattaacc tttacctacg aaccaaaagt attgcgtcac ttcaccgcaa
     1741 aactgaaaga agtttaatta atactacaga gtggctataa ggatgttagc cactctctta
     1801 ccctacatcc tcaataacaa aaatagcctt cctctaaagg tggcatcatg actgatcaag
     1861 ctgaaaaaaa gcactctgca ttttggggtg ttatggttat agcaggtaca gtaattggtg
     1921 gaggtatgtt tgctttacct gttgatcttg ccggtgcctg gtttttctgg ggtgccttta
     1981 tccttatcat tgcctggttt tcaatgcttc attccgggtt attgttatta gaagcaaatt
     2041 taaattatcc tgtcggctcc agttttaaca ccatcaccaa agatttaata ggtaacacct
     2101 ggaacattat cagcggtatt accgttgcct tcgttctcta tatcctcact tatgcctata
     2161 tctctgctaa tggtgcgatc attagtgaaa cgatatcaat gaatttgggc tatcacgcta
     2221 atccacgtat tgtcgggatc tgcacagcca ttttcgttgc cagcgtattg tggataagtt
     2281 cgttagccgc cagtcgcatt acctcattgt tcctcgggct gaagattatc tcctttgtga
     2341 tcgtgtttgg ttctttcttc ttccaggtcg attactccat cctgcgcgat gccaccagca
     2401 ccactgcggg aacgtcttac ttcccgtata tctttatggc tttgccggtg tgtctggcgt
     2461 catttggttt ccacggcaat attcccagcc tgattatttg ctatggcaaa cgcaaagata
     2521 agttaatcaa aagcgtggta tttggttcgc tgctggcgct ggtgatttat ctcttctggc
     2581 tctattgcac gatggggaat attccgcgcg aaagctttaa ggcgataatc tcctcaggcg
     2641 gcaacattga ttcgctggtg aaatcgttcc tcggcaccaa acagcacggc attatcgagt
     2701 tttgcctgct ggtgttctct aacttagctg ttgccagttc gttctttggt gtcacgctgg
     2761 ggttgttcga ttatctggcg gacctgttta agattgataa ctcccacggc gggcgtttca
     2821 aaaccgtgct gttaaccttc ctgccacctg cgttgttgta tctgatcttc ccgaacggct
     2881 ttatttacgg gatcggcggt gccgggctgt gcgccaccat ctgggcggtc attattcccg
     2941 cagtgcttgc aatcaaagct cgcaagaagt ttcccaatca gatgttcacg gtctggggcg
     3001 gcaatcttat tccggcgatt gtcattctct ttggtataac cgtgattttg tgctggttcg
     3061 gcaacgtctt taacgtgtta cctaaatttg gctaaatcct tcaagaagcc agccattcgc
     3121 tggcttcttg cctctcagga aatcacttat gtccaaatgg caactcgcct tatcctcctt
     3181 caccacgtat gctttgcgtc accttactat caggacgctt tagcccatgt cccgcttttt
     3241 gatttgtagt tttgccctgg ttttacttta tcccgccggg attgatatgt acctcgttgg
     3301 tttaccgcgc atcgccgccg atctcaatgc cagcgaagcg cagttgcata ttgcgttctc
     3361 cgtatatctg gcggggatgg cagctgcgat gctatttgcc ggtaaagtgg ccgatcgttc
     3421 agggagaaag ccggtcgcca tacccggcgc ggcgctattt attattgcct cggtgttctg
     3481 ttcactggct gaaaccagca cgttatttct tgcaggccga tttctacagg ggttgggcgc
     3541 aggctgttgt tacgtggtgg cgttcgccat tttgcgcgac acgctggatg atcgacgtcg
     3601 ggctaaagtg ctgtcattac tcaacggtat tacctgcatc attccggtgt tagcgccagt
     3661 gctcggacat ctgattatgc ttaaattccc gtggcagagt ctgttctggg cgatggcaat
     3721 gatgggcatc gcggtactga tgttgtcttt gtttatttta aaagaaacgc gcccagcgtc
     3781 ccccgctgct tcggacaaac cacgagaaaa tagcgagtcg ctgcttaatc gttttttcct
     3841 cagccgtgtt gttatcacca ccctcagcgt ttcggtgatc ctcactttcg tcaatacatc
     3901 gccggtattg ctgatggaaa tcatggggtt tgagcgcgga gaatacgcca ccattatggc
     3961 gctgaccgct ggcgtcagca tgaccttttc attctcgacg ccatttgcgc tgggaatttt
     4021 taagccacgt acgttgatga tcacctcgca ggtgttattc cttgcggcgg ggatcaccct
     4081 tgccgtttca ccttcccatg cggtttctct gtttggtatc acgctgattt gcgccggttt
     4141 ctcggtaggt tttggcgtag cgatgagtca ggcgttagga ccattttcat tacgcgcggg
     4201 cgtagccagc tcgaccttag gtattgcgca ggtttgcggt tcgtcactgt ggatttggct
     4261 ggccgcggtg gttggtatcg gcgcatggaa tatgctgatc gggattctga ttgcctgtag
     4321 catagtgagc ctgttgctga ttatgttcgt cgcgcctgga cgccccgttg ccgctcatga
     4381 agaaatccat caccacgctt gatctcaatc tgctgctctg tctgcaactg ctgatgcagg
     4441 agcgcagcgt gaccaaagcg gcgaagcgga tgaacgtgac cccttcagcg gtgagtaagt
     4501 cgctggcaaa gttaagagcg tggtttgacg acccgctctt tgtgaactca ccgctggggc
     4561 tgtcgcccac acagctaatg gtcagcatgg agcaaaatct ggcggaatgg atgcaaatga
     4621 gcaatctgct gctggataaa ccgcaccacc agacaccgcg cggcctgaag tttgagttgg
     4681 cggcggaatc gccgctgatg atgatcatgc ttaatgcgct gtcgaaacgg atctaccaac
     4741 gttacccgca ggcgaccatc aaactacgta actgggatta cgattcctta gatgccatta
     4801 ctcgtggtga agtggatatt ggtttttccg gtcgcgaaag ccatcctcgc tcgcgggagc
     4861 tgttaagctc gctaccgtta gccattgatt atgaagtgct gtttagtgat gtaccctgcg
     4921 tctggttacg caaagatcat ccggcactgc atgaaacgtg gaatctggac acctttttac
     4981 gttatccgca tatcagcatt tgctgggaac agagcgacac ctg
//
LOCUS       DAJTQY010000183         5013 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.184, whole genome shotgun sequence.
ACCESSION   DAJTQY010000183 DAJTQY010000000
VERSION     DAJTQY010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5013
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.184"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(<1..220)
                     /locus_tag="OO349_003881"
     CDS             complement(<1..220)
                     /locus_tag="OO349_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5868778.1"
                     /translation="MIKPFLMTVAILTSCCAQAKILTNEEIVSRFISNVKTSADNDVD
                     LDTDGFRQLRISCPSPSASGAIILRKASY"
     gene            complement(288..2237)
                     /locus_tag="OO349_003882"
     CDS             complement(288..2237)
                     /locus_tag="OO349_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR5868779.1"
                     /translation="MADSFQLKAIITAVDKVSAPLKGMQRQLKGFKKEFASLSLGAAG
                     AGTAVLGALALPVKSAIALESKMADVRKVVDGLDTPEAFKAMTEQVRDLSTELPMSAE
                     GIAEIVAAGGQAGIARDELMQFTDDAVKMGVAFDTTAEESGQMMAQWRTAFKLTQGEV
                     AGLADKINYLGNTGPASAKKISDVVTRIGPLGSVAGVASGEIAAMGATIAGMGVESEI
                     AATGIKNFMLSLTARDSATKSQKKVLRSLRISPKKLAADMQKDARGAMLHVLDSLAKV
                     PKEKQAAVLKALFGKESLGAIAPLLTNLDLLRTNFNRVADAQQYGGSMQKEYAARAAT
                     TENQLLLLQNQINAISSTLGETFLPSLNEGIKEMKPFLEEVRTFVRENPEVVKTIAKT
                     GAALLTMGVAIGTLTRITKIMGSVMNMTPAKGLIALLVGGAYLIIDNWETVGPVVKKV
                     WQEVDQVVRAMGGWEQAVKTIATVSALYIGVKAVASIRAATVAQNQWTTAAGKTGLKL
                     KGLGKISLIGGLLELGMMAQEFEKEHPWLVKNFVADALNSGFGLNDKFDEWGKQFHDF
                     VYDMTGWQMPRGDGYLSPDKRYTPNVSLERNQLLSLASSPATRSELKVTFDNAPPGMR
                     VIDLPKTGDPFMKITHDVGYSPFKR"
     gene            complement(2322..2657)
                     /locus_tag="OO349_003883"
     CDS             complement(2322..2657)
                     /locus_tag="OO349_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HCR5868780.1"
                     /translation="MAMNVTEIVLKKPVTAHNEMLHVLELREPTYDEIEALGFPFIIS
                     GEGSIKLDSQVALKYIPLLAGIPCSSAAQMAKLDIFKTSMQILRFFTQSETGSTSGND
                     STMLPGSGN"
     gene            complement(2657..3013)
                     /locus_tag="OO349_003884"
     CDS             complement(2657..3013)
                     /locus_tag="OO349_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HCR5868781.1"
                     /translation="MAKIAGTCFFKVDGQQLSLTGGIEVPMNTNVRDDIVGMAGDVDY
                     KETWRSPYVKGTFKVPKNFPVDKITTSDQMTITAELANGMVYVLSAAWLHGEANHNAE
                     EGTADLEFHGEEGGYQ"
     gene            complement(3013..4509)
                     /locus_tag="OO349_003885"
     CDS             complement(3013..4509)
                     /locus_tag="OO349_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HCR5868782.1"
                     /translation="MTISFNTVPSNTLVPLFYAEMNNSAANTAVTSAPALLIGHASND
                     AAIEVNSLVLMPSADYARQICGAGSQLARMVEVYRQTDPFGELYVIAVPEARGAAATV
                     RVTVAGEAEESGTLSLYVGRSRVQVPVVNGDDATAVATAIKEAVNGVITLPFTASSDA
                     GVVTLTARHKGLYGNELPVCLNYYGSGGGEILPAGLRVVTEVGAAGSGAPDLTAAVAA
                     MGDEAFDFIGLPFNDAASINMMMTEMNDSSGRWSYARQLYGHVYTAKLGTLSELVGAG
                     DMHNQQHITLAGYEKETQSPVDELVASRLAREAVFIRNDPARPTQTGELVGMLPAPKG
                     KRFIMTEQQTLLSHGVATAYVEGGTLQIQRSVTTYKKNAYGVADNSYLDSETLHTSAY
                     VLRKLKSVITSKYGRHKLANDGTRFGPGQAIVTPAVIKGELLATYRQMERAGIVENYD
                     LFKQYLIVERDADNPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
     gene            complement(4506..4667)
                     /locus_tag="OO349_003886"
     CDS             complement(4506..4667)
                     /locus_tag="OO349_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HCR5868783.1"
                     /translation="MFVKPLKGRSVPDPARGDLLPSEGRNVEESSYWFRRIAAGDVVR
                     VKQDKAKKS"
     gene            complement(4677..>5013)
                     /locus_tag="OO349_003887"
     CDS             complement(4677..>5013)
                     /locus_tag="OO349_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5868784.1"
                     /translation="RDEKGQWAAYDAVHDVRRELWKALLGWMPDPQGGEIVYVGGTLL
                     DLNRYELYYQFDFTAKYEITEEDTRQAEDVNALPDLSLLSIDVDYIDPGTGPDGDIEH
                     HLEMRFPQN"
BASE COUNT         1166 a         1488 c         1104 g         1255 t
ORIGIN      
        1 cataagaagc ttttctcaat ataatcgcac cgctggcaga aggggatggg cacgatatcc
       61 tcagttgtct gaatccatct gtatccaagt caacgtcatt atctgcagat gttttaacgt
      121 tactaataaa tcgtgaaaca atctcctcat ttgttagtat tttcgcctga gcacagcaag
      181 aggttaatat tgcaaccgtc attaaaaatg gctttatcac aaaacaaact ccattgttag
      241 catgttacgc gataattttg cctcatcttt caccacaaaa aaagaactca ccttttaaat
      301 ggagaatacc caacatcatg ggtgattttc ataaagggat cgcctgtttt cggcaaatcg
      361 ataacgcgca ttccaggcgg cgcgttgtca aacgtcacct taagttcgct gcgggttgcc
      421 ggtgaagatg caaggcttaa cagctgattc ctttccagtg atacattcgg ggtgtagcgt
      481 ttatcgggag ataaataccc atcaccacgt ggcatctgcc atccggtcat gtcatagaca
      541 aaatcatgga actgtttgcc ccactcgtcg aacttatcat tcagaccaaa tccactgttc
      601 agtgcatcag ccacaaaatt tttaacaagc cagggatgtt ctttttcaaa ctcctgcgcc
      661 atcatgccca gttcaagcaa tccaccaatc agactgatct ttccgaggcc ctttaatttc
      721 agccctgttt ttcctgccgc cgttgtccat tgattctggg cgactgttgc cgcacgaata
      781 cttgccaccg ccttcacacc aatatacagg gcagaaaccg ttgctattgt ttttactgcc
      841 tgttcccatc cccccattgc tctgacaacc tgatcaacct cctgccagac ttttttcaca
      901 accggaccga ccgtttccca gttatcaatt atgagatatg caccaccgac gagaagcgca
      961 attaatccct ttgccggcgt catattcatc acgctaccca tgatttttgt tatgcgtgtc
     1021 aatgtgccaa tcgccacgcc catcgtcagt aatgccgcac cagttttcgc gatggttttt
     1081 acgacctcag gattttctct gacaaacgtg cgcacttctt ccagaaaagg cttcatctct
     1141 tttatgcctt cattgagtga aggcaggaag gtttccccca gcgtggaaga aatcgcattg
     1201 atctggttct gcagcagcaa caactgattt tctgtcgtcg cggcacgggc ggcatattct
     1261 ttctgcattg agccgccata ctgctgcgca tccgccacac gattaaagtt ggttcgcaac
     1321 aaatccagat tcgtgagcag cggcgcaatg gcccccaggg attccttccc gaacagcgct
     1381 ttcagcacag cagcctgttt ttctttcggc acttttgcca gagaatccag tacgtgcagc
     1441 atggccccgc gggcgtcttt ctgcatatcc gccgccagtt ttttcgggct aatgcgcaac
     1501 gagcggagca cttttttctg cgatttggtg gcagaatccc tcgctgtcag cgacaacata
     1561 aaattcttta tccccgtcgc agcaatttct gactccaccc ccattccggc aatggttgcc
     1621 cccattgcgg caatctctcc ggaggccaca cccgcaacac tgcctaaagg gccaatacgg
     1681 gtcacaacat cagaaatctt tttcgcactt gcaggaccgg tattgccaag atagttaatc
     1741 ttatccgcaa gccctgccac ctctccctgt gtcagtttaa aggcagtgcg ccactgcgcc
     1801 atcatctgac cggattcttc tgccgtggtg tcaaaggcca cgcccatctt cacggcatcg
     1861 tcagtaaact gcatcagctc gtcacgggcg ataccagcct gaccacccgc cgccacaatt
     1921 tcggcgatac cttccgccga catgggcagt tctgttgaca ggtcgcgcac ctgctccgtc
     1981 attgccttaa acgcttccgg cgtatccaga ccatccacca ctttccggac atccgccatt
     2041 tttgattcaa gggcaatggc agatttgacc gggagcgcca gcgcccccag tactgcggtt
     2101 ccggcacccg ctgcgcccag tgacaggctg gcaaactcct ttttaaaacc tttcagctgg
     2161 cgctgcattc ccttcagcgg tgccgatacc ttatccacgg cagtgatgat cgccttcagc
     2221 tgaaaactgt cagccatgct tcatctcctc gttaatgcgg acggcctcgg cttccagttc
     2281 tgtaaactgc gaaacagcca cccgtcgcag ctccagtgga ttcagtttcc agaaccaggc
     2341 aacattgtag agtcgtttcc ggaggtgctt cccgtctccg actgggtaaa aaaacgcagg
     2401 atctgcatgc tggtcttaaa aatatccagt tttgccatct gcgccgccga tgaacacggg
     2461 atccccgcca gcaacgggat atatttcagt gccacctggc tgtccagttt aatactgcct
     2521 tcaccggaaa taatgaaagg aaaacccagc gcctcgattt cgtcatacgt gggctcgcgc
     2581 aactccagca catgcagcat ttcgttatgc gctgtcaccg gtttttttaa aactatttct
     2641 gtaacgttca ttgccattac tgataccctc cctcttcgcc gtggaattca agatctgccg
     2701 tgccttcttc ggcattatga ttagcctctc cgtgcagcca tgccgccgaa agcacataca
     2761 ccatgccgtt tgccagctca gcggtaatcg tcatctggtc tgaggtggta attttgtcga
     2821 ccggaaagtt tttgggcact ttaaacgtgc ccttaacgta aggtgaccgc caggtctcct
     2881 tgtaatccac atcccctgcc atgccgacga tgtcatcccg gacattggtg ttcatcggca
     2941 cttcaatgcc acctgtcagc gataactgct gaccatccac tttaaaaaaa caggttccgg
     3001 cgatctttgc cattatgctg actcctctga atactgaaga cggaactggt taaccaccgc
     3061 aaagacacgt aactgattaa cataatccgg cgggaacagc gtattcagtc ggttcgggtt
     3121 atctgcatca cgctcaacta tcagatactg tttaaacaga tcgtaatttt ccacaatacc
     3181 ggcacgctcc atctgacgat atgtcgccag aagttcccct ttgataacgg caggagtgac
     3241 aatcgcctgc cccggaccaa aacgggtacc atcatttgcc agcttgtgac gtccgtactt
     3301 gcttgtgatg accgatttca gtttgcgaag aacatatgca ctggtatgca gagtttcact
     3361 gtccagatag ctgttgtctg ccacgccata cgcattcttt ttgtaggtgg ttacggaacg
     3421 ctggatctgc aacgtgccgc cttccacata agccgtcgcc acgccgtgag ataaaagggt
     3481 ctgctgctct gtcatgatga atcgcttacc tttcggtgcc ggaagcatcc ccaccagctc
     3541 ccccgtctgt gtcggacggg caggatcatt ccggataaat accgcttcac gggcaaggcg
     3601 actggcaacc agttcatcga caggcgactg ggtttctttt tcgtaaccgg caagcgtgat
     3661 atgttgctga ttatgcatat ctccggcacc aaccagctct gacagcgttc ccagttttgc
     3721 ggtatagaca tgcccgtata actggcgcgc ataactccag cgaccgctgc tgtcattcat
     3781 ttcggtcatc atcatattga tggaggcggc atcgttgaac ggcagaccga taaagtcgaa
     3841 tgcctcatcg cccatagcag caacagcggc ggtaagatca ggcgctccgc taccggcagc
     3901 accgacttcc gtcaccaccc gaagccctgc tggcaaaatc tcaccaccac cagaaccata
     3961 ataattcaga cagaccggta actcattacc atacagcccc ttatgacggg cagtcagcgt
     4021 caccacgcct gcatcagatg acgccgtaaa cggcagggtg ataaccccgt ttaccgcttc
     4081 cttaatcgcg gtggcaaccg cagtggcatc atcgccattc accacaggca cctgtacacg
     4141 ggagcgcccg acatacagac tcagggtgcc gctttcctct gcttctccgg caaccgtcac
     4201 cctgaccgtc gccgccgccc ctctggcttc cggtactgca ataacataca gttcaccgaa
     4261 aggatctgtc tgacggtaga cttccaccat acgcgccagc tggctccccg ccccacaaat
     4321 ctggcgggca taatctgccg atggcatcag caccaggctg ttaacctcaa tggctgcatc
     4381 gttgctggca tgcccgatca gtaatgcagg cgcgctggtc accgctgtat ttgccgcaga
     4441 attgttcatc tcggcgtaga acaacggcac cagcgtattc gacggaacag tattaaaact
     4501 tatcgtcatg attttttagc cttatcctgt ttaacacgta ccacatcacc cgccgctata
     4561 cggcggaacc agtagctgct ttcttccaca tttcgccctt cggaaggcaa aaggtctcca
     4621 cgggcagggt caggaaccga ccgccctttc aggggtttca caaacatgag ggactcttaa
     4681 ttctgaggga aacgcatttc cagatggtgc tcaatgtcac catccgggcc agtaccagga
     4741 tcgatgtaat ccacatcaat actcagcagg gataaatccg gcagggcatt cacgtcctct
     4801 gcctgtcgcg tgtcttcttc cgtgatttca tacttcgccg taaaatcaaa ctggtaatac
     4861 agttcgtaac ggttcagatc cagcagggtg ccaccgacat aaacaatctc gcccccttgc
     4921 gggtccggca tccatccaag cagggctttc cagagttctc tccggacatc atgaacggcg
     4981 tcataggctg cccactgccc tttttcatca cgc
//
LOCUS       DAJTQY010000184         4997 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.185, whole genome shotgun sequence.
ACCESSION   DAJTQY010000184 DAJTQY010000000
VERSION     DAJTQY010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4997
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.185"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            <1..239
                     /locus_tag="OO349_003888"
     CDS             <1..239
                     /locus_tag="OO349_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HCR5868785.1"
                     /translation="LSPVVMLAKSVTAQTHMAWSDADNQQVKLSMPELEELAAAMVQA
                     QVDRNDEIYRRQRELKEELNSLKDLNSVRNFIVE"
     gene            complement(440..1297)
                     /gene="sitD"
                     /locus_tag="OO349_003889"
     CDS             complement(440..1297)
                     /gene="sitD"
                     /locus_tag="OO349_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HCR5868786.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
     gene            complement(1294..2151)
                     /gene="sitC"
                     /locus_tag="OO349_003890"
     CDS             complement(1294..2151)
                     /gene="sitC"
                     /locus_tag="OO349_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HCR5868787.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            complement(2148..2975)
                     /gene="sitB"
                     /locus_tag="OO349_003891"
     CDS             complement(2148..2975)
                     /gene="sitB"
                     /locus_tag="OO349_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HCR5868788.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREAR"
     gene            complement(2975..3889)
                     /gene="sitA"
                     /locus_tag="OO349_003892"
     CDS             complement(2975..3889)
                     /gene="sitA"
                     /locus_tag="OO349_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HCR5868789.1"
                     /translation="MHSIKKVTMLLGCLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
     gene            complement(4362..>4997)
                     /locus_tag="OO349_003893"
     CDS             complement(4362..>4997)
                     /locus_tag="OO349_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR5868790.1"
                     /translation="PLSKTEFNALMESEKGQSQNLWKFAVYSGLRHGELAALAWEDVD
                     FEKGIVNVRRNLTILDMFGPPKTNAGIRTVTLLQPALEALKEQYKLTGHHRKSEITFY
                     HREYGRTEKQKLHFVFMPRVCNEKQKPYYSVSSLGTRWNAAVKRAGIRRRNPYHTRHT
                     FACWLLTAGANPAFIASQMGHETAQMVYEIYGMWIDDMNDEQIAMLNARLS"
BASE COUNT         1332 a         1218 c         1196 g         1251 t
ORIGIN      
        1 gcctgtcccc ggtagtcatg ctggcaaaat ctgtcacagc acaaacacat atggcgtgga
       61 gcgatgccga taatcagcag gtgaaactgt cgatgccgga actggaagaa ctggcggcag
      121 caatggtgca ggcgcaggtc gatcgcaacg atgagattta tcgccgtcag cgggagctga
      181 aggaagagct gaatagtctg aaggatttga attcggttag gaattttatc gtggaataac
      241 agaagctgcg gcacgtcgta tgcaggaacg tgccgcggtt ggctggtaaa ctttcgatag
      301 tgcgagtatt gaatgatttc taggcgttat cgattttacg tattttttgc atgatagaat
      361 tcgtacctcc tcccaacgac cttccatggc tttccggact tctgtaggct ggcggacgat
      421 gctgtcctcg gatgttgtgc taattgggtg ataacagatc ctgtgtatgg gcgtttccct
      481 ttgtgcgtgc gttgatgctg gtaacggtaa acgtcatgat gaacacgacc gcgaataaga
      541 cgacaatcgt cggagctggt gcgctgtcga taaaaaagga cagccagaca ccagtcattg
      601 acactattac cgatatgcca gtcgccagta ggagcgcaat atgaaagcgt tgtgtcagga
      661 gtactgcaat ggcgccaggc gcaatgagca gagaaataga gagaatgata cctactgctt
      721 tcagcgtcgc cacaatggtc agggaaacca tgcacagcag cccataatgc agccatctcg
      781 tatgcaaacc gcttacctgc gcctgttgat aatcaaagct gaacagcaaa aaatcccgcc
      841 attttacgct aataacaagt gtaaccagcc cggcaataat caccgtctgg attatatcgc
      901 cgatggttat cccgagcatg tcgccgaaaa gaatatggtc aagatgtacg tctggtttaa
      961 ttgcaatata caagatcaga cctgcggcaa acatgccaga aaaaacaatc cccataactg
     1021 tatcttgctt gattcggctg ttgtctttca ggtatcccgt cgccaccgcg cagaatactc
     1081 cggcgacgaa tgcccctgta gccagaggta accccagaat ccaggtaagc acgatgccag
     1141 gaaaaaccgc atgactcatc gcgtcaccca tcagtgccca acctttcagg accagaaaga
     1201 ccgacagcag cgcacagggt atggaaacta ccagtgaaat cagcagtgcg tgactcataa
     1261 aggtgaactg cagtggctcc agtaataatg ccatcatgag cgatcctcca gcgctttatg
     1321 cgcacgccga cgatttgcca aaagtccatg ggtgggcgca aatacaaatg ctaaaagaaa
     1381 cagcagtgtc tgcgccacca cgatgattcc acctgtcgcg ccgtccagaa agtaactgac
     1441 ccacgcgccg aggaaactgg ttacactgcc aatagttaca gcaattatga gcaggcgggg
     1501 aaaacgatcg gttaacaacc atgctgttgc accgggagtc accaccagac agatcaccag
     1561 aaacgcacca accgtttgca gcgccgcaac ggtcgaaacc gacagcaggg tgaagaagag
     1621 gatttttaat ctgcccggat gtaatccaat agcacgggcg tgattctcgt cgaaaaaggt
     1681 caccataaga tctttccatt tgaaaaaaag cacgatgatc gagagaatgc caatgatagt
     1741 gagttgcagg atatccgccg gatcgattgc cagaatattg cccaggacga tggtctgaat
     1801 gttcacggat gtcgggttca atgacaccat aaacagcccc aggccaaaaa aggacgagaa
     1861 gatcaggccg atgatggcat cttcttttaa gcgggtgcgt tggttaagaa acagcatgct
     1921 gcccgctgcc agtccgccgg aaaagaaggc tcccagagaa aacggaagcc ccagcatata
     1981 tgcccccgca acacccggga caatagagtg cgacagcgca tcgccaatca gcgaccagcc
     2041 ttttagcatc agatagcatg acaggaaagc gcagagaccg ccgaccattg ccgagaccca
     2101 catcgcattg agcatatatt catagctgaa gggttccagt agcacgttca tcttgcttcc
     2161 ctctgcaccg ctgacggtcg atgtgccaca aaagggcgtt catcgtcagt aataatgctt
     2221 tcttccgagc cgttaagtgt gacatggcgc agtacgccgc taaaagccag ctccaggttc
     2281 tcggctgtaa aagtggtgtc tgtcggcccg cttgccagga cggtgccttt gaccatgacc
     2341 gtataatcgc aaaatgtcgt gactgagccg agattgtgcg ttgagacaag catcgttttg
     2401 ccttccgcgc gtaactccct caacaggctg atgattttcg cttcggtttt cacatcaacg
     2461 ccggtaaaag gttcatccag caggatcaca tcaccctgct gtgcaattgc tctcgccaga
     2521 aagacgcgtt tcttttgtcc accggaaagc tcgccgattt gacgatggcg aaaatccacc
     2581 atatcgacac gttccagcgc atcggtgaca atctgacgat ccctcttttt ggctatacgc
     2641 aacataccca tatgcccata gcggcccatc atcaccacat cttccaccag tacaggaaat
     2701 gaccagtcaa cctcttctga ctgagggacg taggcaacca ggtttttctg tagcgcctgt
     2761 cgcgtgggaa tacccagaac agatatcttt ccgctggtca gacgcacaaa tcccataatc
     2821 gctttaaaca acgtcgattt tccggaaccg tttaccccaa ccagagcggc aatagatcca
     2881 cttggtactg tgaaggatgc atcgcgcagt gctgtgtgcc cgttacgcca ggtgacagta
     2941 acatcattaa cgacaatgcc tgcagattgc atcattattt ctcccttttc ccggctttaa
     3001 ttccctgtac cagcgtactg gtagtaacct taagaaggtc gatatacgta ggtaccgggc
     3061 cgttttctgt gctcagggaa tcgacataga gcacaccgcc gtagtgtgcg ccggtttcac
     3121 gcgcaacctg acgcgctggt ttatcggaaa tcgtactctc gctaaagact gccgggatat
     3181 gatttttctt aactatatca acaaccttac gtacctgctg cggtgttcct tgttgatcgg
     3241 cattaatcgg ccacagataa agctctttta gccccaaatc gcgtgcgaga taagaaaaag
     3301 ccccttcact ggtgaccatc catcgctgat tctcagggag ttccgtaatc tgcttacgca
     3361 ggggggcaag ggtttgggta atcttggctt tataagtatc ggcattacgt tggtaggttt
     3421 gtgcatttgc cggatcgtat tttatcaacg catcacgaat attatcgacg taaatcagag
     3481 cattatctgg cgacatccat gcatggggat taggtttgcc ctcatagggt ccttcggtga
     3541 tccctactgg cgtcacaccc gaagagacaa ttacttctgg aaccccattg agatgctggt
     3601 aaaagcgttg gaaccacaat tccagattca taccattggc gagaatcagt tgtgccccct
     3661 gcgcacgttt aatatcgcca ggggtaggct gatactcatg aatttctgca ccaggcttgg
     3721 ttatggatga gacttctgca gcatctccag ccacgttttt ggccatatcc gcgatgatgg
     3781 taaatgttgt aatgaccttg aatttttcag ttgcacttgc ctgaaatgcg atcgagcagg
     3841 tgagggcgag gcaccccaag agcatggtta ctttttttat tgagtgcata atttagtacc
     3901 tatagtgagc aaaattagaa caaacatagc aacggctatg ttttatagca taacctatac
     3961 aaaatgaaaa taattatcat taacagcttt gaggcctaaa acagggatta ggttgtttcc
     4021 tgctggcgac atgatattga ggaaaaaagt agagcagcta ttaggctttg aaatgacttt
     4081 atgaggaaaa ttgttgatgg caaaaatggg gcaaaacgct gtataagggg caaaaaaggg
     4141 gcaaaaaagg ggcaaaaaag agtgggttat cgtagcttat tgttgtcgct gataatgttt
     4201 aacatattga aaaataaata aaatacttat gagtcagata gttgtgattt ttgcccttac
     4261 ttgttcaggt cgtattgttc tttctttgta atttgttgat tttcttgcat tatttcagtt
     4321 ctctggtact aaatggggca aattgggggc aaactttgca actacgataa ccgcgcattc
     4381 aacatggcta tctgttcgtc gttcatgtca tcaatccaca taccgtaaat ttcatacacc
     4441 atttgcgcag tttcatgccc catctggctg gctataaatg ccgggttcgc tcctgccgtc
     4501 aacagccagc aggcaaaagt atgtcgcgtg tggtacggat tacggcggcg aataccagca
     4561 cgttttactg ctgcattcca tcttgtaccc aaactgctta ccgagtaata aggtttctgt
     4621 ttttcgttac acaccctggg catgaaaaca aaatgcagtt tttgtttttc agttctgccg
     4681 tactcccgat gataaaaagt gatttcgctt ttgcgatgat gcccggtcag tttgtattgc
     4741 tccttcagtg cttcaagagc cggctgcagt aatgttaccg tccggatccc ggcatttgtt
     4801 tttgggggac cgaacatatc aagtatcgtc aggtttcttc tgacattcac aattcccttc
     4861 tcgaaatcca catcctccca cgccagagct gccagttccc cgtgacgaag cccggagtaa
     4921 acggcaaatt tccacaagtt ttggctctgt cctttttcac tttccattaa tgcattgaat
     4981 tctgttttag ataacgg
//
LOCUS       DAJTQY010000185         4974 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.186, whole genome shotgun sequence.
ACCESSION   DAJTQY010000185 DAJTQY010000000
VERSION     DAJTQY010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4974
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.186"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(187..771)
                     /gene="kptA"
                     /locus_tag="OO349_003894"
     CDS             complement(187..771)
                     /gene="kptA"
                     /locus_tag="OO349_003894"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HCR5868791.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADINKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGDRHYVHLSADEATARKVGARHGSPVVLTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAVEFLEWSFMPTGVQKQ"
     gene            1021..1704
                     /locus_tag="OO349_003895"
     CDS             1021..1704
                     /locus_tag="OO349_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HCR5868792.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFPGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGVGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            1701..2162
                     /locus_tag="OO349_003896"
     CDS             1701..2162
                     /locus_tag="OO349_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HCR5868793.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLSGEAATVLLAALMSMGGAVGVAASLATADALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            2175..3347
                     /gene="iadA"
                     /locus_tag="OO349_003897"
     CDS             2175..3347
                     /gene="iadA"
                     /locus_tag="OO349_003897"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCR5868794.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPNCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALKPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNMSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            3412..4323
                     /gene="hypT"
                     /locus_tag="OO349_003898"
                     /gene_synonym="qseD"
     CDS             3412..4323
                     /gene="hypT"
                     /locus_tag="OO349_003898"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HCR5868795.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGGDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMVRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDEQVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(4316..4708)
                     /gene="iraD"
                     /locus_tag="OO349_003899"
     CDS             complement(4316..4708)
                     /gene="iraD"
                     /locus_tag="OO349_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HCR5868796.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(4705..4788)
                     /gene="idlP"
                     /locus_tag="OO349_003900"
     CDS             complement(4705..4788)
                     /gene="idlP"
                     /locus_tag="OO349_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HCR5868797.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
BASE COUNT         1175 a         1303 c         1241 g         1255 t
ORIGIN      
        1 ggtatgaccg gaagcgacgc tgattccagt ccccaactga ccgcctgccg taaatcactt
       61 taatttcagc agttgttatt aaaggatacc cttttacgag ggtatccctt cagactgata
      121 acaaacgcta cattgccgga tgtgctgcgc ttaccaggcc tatataaccc ctgcaatata
      181 ttgaatttat tgtttttgta ccccagtcgg cataaacgac cactcaagaa actcaacagc
      241 aaccgttgat gtcagccaga ccccgttttc cgcttgccag aaagggatgc ctcgtttcgc
      301 catttcctgt gctttgacgg ttaagacaac cggcgatccg tgtcttgctc ccactttgcg
      361 tgccgttgct tcatcagcag agagatgcac gtaatgacga tcccctgcaa tcagcccctg
      421 tttttttatc tcgtcaagga aacggcttgc cgtaccgtgg tagagaaact gtggcggtgt
      481 tttttcggca aaggaaatag cgacctgcga agtcgaatgc ccctgaaccg cgcggatgca
      541 cctaccatca ctggaataac taaaacgctt tttatcgctg gttgcgacca cggtatccag
      601 caatgcacgg gttaatcgct tgcccgcttt ttgggcacaa agtatgagct tattgatatc
      661 cgcccatcct tcacggtcca gtacgatacc aatcgcctcc ggtttgtgtc ggaggacaaa
      721 acttaaaaac ttgctcgtat ccgccagctc tttttcgttg tactttgcca tcgcgttttc
      781 gtccctggat tttctgtttt tatttactaa tgtcattgtc actgggttta tgaattttct
      841 gcaactcaat cctgcgcaga accttttcca cacagcgtaa tttttcccct atgcaaaaaa
      901 cgcatagcca gaaacatact tgcattttcc ccatacccac atggctgata aggtttttca
      961 gttccaggaa ttttcctggc tcaagcaaca cacaaacaca aaaaaacaat ggggattgtt
     1021 atgacacaac aaggggatgc tgtagccggg gagctggcga cagaaaaagt gggcattaaa
     1081 gggtatctgg ctttttttct caccataatc ttttttcccg gtgttttttc cggaacagac
     1141 agctggtggc gcgtttttga tttttccgtt ctgaacggat cgtttgggca actgcctggt
     1201 gcgaatggag ccaccacctc atttcgcggg gtgggtggcg cgggggctaa agatggcttt
     1261 ctctttgcgc tggagctggc accgtcagtc attctctctc tggggattat ttcaattacc
     1321 gatggtcttg gtggattacg cgccgcgcag cagttaatga cacctgtgct aaaaccgttg
     1381 ttgggcattc cagggatctg ctcgctggca ctgatcgcta acctgcaaaa cacggatgcc
     1441 gccgccggta tgaccaaaga gctggcgcag gaaggtgaaa ttaccgaacg cgataaagtg
     1501 attttcgccg catatcaaac cagcggtagc gccatcatca ccaactactt ctcttccggc
     1561 gtcgcggtgt ttgcctttct gggcacgtcc gtaatcgtac ctttagccgt catcctggtg
     1621 tttaaatttg tcggagccaa cattttgcgc gtctggctta acttcgaaga acgtcgcaac
     1681 ccaacgcaag gagcgcaagc atgacaactc aggtccgcaa aaacgtcatg gatatgttta
     1741 tcgatggcgc gcgccgtggc tttactatcg ccacgaccaa cctgctgcca aacgtggtga
     1801 tggcgtttgt catcattcag gcgctgaaaa ttaccggcct gctcgactgg gtggggcata
     1861 tctgtgaacc cgtaatggcg ctgtggggat tatccggtga agcggcaacc gtccttttag
     1921 ccgcattaat gagtatgggc ggcgcagtcg gtgttgccgc cagtctggca accgctgacg
     1981 cattaaccgg ccacgatgtc accgttttac tgcccgcaat gtatttaatg ggcaacccgg
     2041 tgcaaaatgt cggtcgctgc ctggggacag ccgaagtgaa tgccaaatat tacccgcata
     2101 tcattacggt ctgcgtgatt aacgcattgc tgtcgatctg ggttatgcag cttattgttt
     2161 aacaaggagt taccatgatt gattataccg cagccggttt taccctgctg cagggagcgc
     2221 atttgtatgc gccggaagat cggggaattt gcgatgtcct cgtcgctaac ggcaaaatta
     2281 tcgccgttgc cagcaatatc ccttctgaca ttgtaccgaa ctgcacggtt gtcgatctca
     2341 gcgggcagat cctctgccca ggttttattg atcaacacgt ccatttgatt ggcggtggcg
     2401 gcgaagcagg tcccacgacg cgcacgccgg aagtggcgct aagtcgcctg acggaagcgg
     2461 gcgtcacgtc agtggttggt ctgctgggca ccgactctat ctctcgccac ccggaatccc
     2521 tgctcgccaa gacccgtgcg ctcaatgaag aaggcatcag cgcctggatg ctgaccggcg
     2581 cttatcatgt cccttcccgc actattacgg gttccgtgga aaaagacgtg gcgattatcg
     2641 atcgtgtgat tggcgtgaaa tgcgccatct ctgatcaccg ttctgccgca ccggacgttt
     2701 atcacctggc taatatggcg gcagaatccc gcgttggcgg tttgctcggc ggtaaacctg
     2761 gcgtcaccgt gttccacatg ggcgacagta aaaaggcgtt aaagcctatt tatgacctgc
     2821 tggaaaactg cgatgtgccg atcagcaagc tgctgccgac ccacgttaac cgcaacgtac
     2881 cgttgtttga gcaggcgctg gagttcgcgc gcaaaggcgg caccatcgat atcaccagca
     2941 gcattgacga accggtcgcc cctgccgaag gtattgcccg cgccgttcag gcgggtattc
     3001 cgctggcacg cgtcaccctc agctccgacg gcaacggtag ccagccgttc ttcgatgacg
     3061 aagggaattt aacccatatc ggtgttgcag gttttgaaac gttgctggaa accgtgcagg
     3121 tgctggtcaa agactatgat ttcagtatca gcgatgccct gcgcccgctc accagtagcg
     3181 tagccggttt ccttaacatg agcgggaaag gcgaaattct gccaggcaat gacgcagact
     3241 tactggtcat gacgccagaa ttgcgcattg agcaggtata cgctcgcggc aaactgatgg
     3301 tcaaagacgg caaagcctgc gtgaaaggaa cgtttgaaac ggcttaaacg gttgtcagcc
     3361 gccctaatgg acgtagaatg cccccaaggg cggctgacag agtaaaacgt aatggatgac
     3421 tgtggtgcgg ttttgcataa tattgaaacc aaatggcttt atgattttct gactctggaa
     3481 aaatgccgca atttttccca ggcggcagtc agtcgcaacg tctcgcaacc ggcattcagc
     3541 cgccgcatcc gtgcactgga acaggcgatt ggcgttgaat tgtttaaccg ccaggtgacg
     3601 ccactgcaac tctcagaaca agggaaaatc ttccattcgc agatccgcca tctgttgcaa
     3661 cagttagaaa gcaaccttgc agagctgcgt ggcggcggcg attacgcgca acgtaaaatc
     3721 aagatagccg ctgcacactc tctttccctc ggtctgttac cgtccattat cagccagatg
     3781 ccgccgctct ttacctgggc aattgaagcc attgatgtcg atgaagcggt cgataaactg
     3841 cgtgaagggc aaagtgactg tattttttcc tttcacgacg aagatttgct ggaagcgccg
     3901 tttgaccaca ttcgcttatt tgaatctcaa ttgttccctg tctgcgccag tgacgaacac
     3961 ggagaagcac tttttaacct cgcgcagcca cactttccgt tactgaatta cagccgcaac
     4021 tcctacatgg tgcgattgat taatcgcacc ctgacgcgcc acagtgagtt aagtttcagc
     4081 accttttttg tctcttcgat gagcgagctt ttaaagcagg ttgccctcga cggctgtggg
     4141 attgcctggc tgccggagta tgccatccaa caagaaattc gcagcgggca gctcgttgtg
     4201 ctgaatcggg acgaacaggt gatcccgatt caggcctacg cataccggat gaatacccgc
     4261 atgaatccag ttgccgaacg cttctggcgt gaactgcgcg agctggagat tgtgcttagc
     4321 tgacattctc aagcgtcgca ctgcgccaac cgccattatc ccagaccaga tcaaacgtta
     4381 gcggttcatc gtcgtggtac agcattatct caaggctgat cgccagagtg tggcagtacg
     4441 cttctttcaa attaacctgc aacgccgcaa tccgtggctc atgaaataac agtgcagaac
     4501 gcagctgacg gcaataccag ctctggagat gtttgtcgct cgggttatgc acttgccact
     4561 cgggcatgcc tgaaacaagt aatgacggta atgccgaatg aggagaatta aaaagagtga
     4621 ggagatcgct gcataccgat ttacgcagca atgacgtttt attgcctgaa atttcaggta
     4681 aaacagcctg aagtgattgg cgcatcattt tgcgcactcc tgacgtttag caacatatgc
     4741 ggcttgccct gaacaccggg caccactgta ttgatgctca ttttccatag ccattccctt
     4801 ttgttcatta catccatgtc agtctgagac aggcctgaat cctgtcagca aagtataaaa
     4861 ccttacttgc ctatgaatat ctactgactt ttcaggaaaa aagtcatgat ttgataaaaa
     4921 tcatcaaact caaaaaaaaa acgctagtat attctgaata ttccgaatag ttac
//
LOCUS       DAJTQY010000186         4868 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.187, whole genome shotgun sequence.
ACCESSION   DAJTQY010000186 DAJTQY010000000
VERSION     DAJTQY010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4868
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.187"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            <1..971
                     /locus_tag="OO349_003901"
     CDS             <1..971
                     /locus_tag="OO349_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR5868798.1"
                     /translation="AEMFVFLAVELSLLFIVISAGVSLIRQKVPDHKIQQMMGARKGR
                     GYLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLNPIIVGLMWMTF
                     GWKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCAPAKTSPGTTYTPIK
                     VSCCSPAAKAIENPVVTCCSTKAVVSINPIKLATKDALQQFKDVLPYLLLGVLIGSFI
                     YGFIPSAWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMTKGMGLGALMAL
                     IIGSAGASLTEVILLKSMFRMPMIAAFLTVILGMAILMGYLTQLLF"
     gene            1552..2775
                     /locus_tag="OO349_003902"
     CDS             1552..2775
                     /locus_tag="OO349_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5868799.1"
                     /translation="MSIDFTPGIINTYHGDIYNCTTNTDNVKTPDTPKWPCDNWEEQQ
                     PINSTFSGEGYNPEQFDLAQQQLQQINACHTNTTYTNADYSKVVVQLVSLINNIETIS
                     STPLTQQTQSILNQIHNIRYEKDKDSVCQIIVVVNPEPNNPIITKILVEEGIPERFSV
                     QTVSSDNKNFAGQRADLPTDIRDIQSLYLKMAKLYIEHSKNENRMEALAGCDFIDFNM
                     TGQDMSKLVLTLSKFYFEDLLNINFTDANLFNTIFSHKENPIPKLHKYEQHLDKQING
                     LFSTLLTINDNSLRAKAEIASRIIDFLEAKVVNLSFDDILKYKQEFKKICYKQLQEFT
                     TPSLYNKIQKWATMSKNEFIAFHYETLQPEKISYPFYLKRDLPNEKDINYGVEIEIPS
                     GKRIRLSNHYQNIIP"
     gene            3022..3588
                     /gene="slp"
                     /locus_tag="OO349_003903"
     CDS             3022..3588
                     /gene="slp"
                     /locus_tag="OO349_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HCR5868800.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
     gene            3744..4274
                     /locus_tag="OO349_003904"
     CDS             3744..4274
                     /locus_tag="OO349_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5868801.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(4316..4868)
                     /locus_tag="OO349_003905"
                     /pseudo
     CDS             complement(4316..4868)
                     /locus_tag="OO349_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="MgtC/SapB family protein"
BASE COUNT         1556 a         1000 c          905 g         1407 t
ORIGIN      
        1 ccgcagagat gtttgtgttt ctcgccgtcg agctttcgtt gctgtttata gtgattagtg
       61 ccggtgtcag cctgataaga caaaaggtgc cagaccataa aatccagcag atgatggggg
      121 ccagaaaagg gagaggctat ctcctggctg ctctgttggg agccgttacc ccgttctgta
      181 gttgctcgac aatacccatg ttacgtggat tgttgtcagc gaaagccggg tttggtccga
      241 ctctcacttt tttatttgtt tcaccattac ttaatcccat tatcgtcgga ctaatgtgga
      301 tgacctttgg ttggaaagtc accttgttgt acgcgattat cgcagccggc gtctccgttc
      361 ttgccagtat tatcctggat tctctggggt ttgaacgtca tatcattgcc agtaaaagct
      421 catcagcaaa ttgttgtgct ccagcaaaaa cttcgccggg aacgacatat acgcccataa
      481 aagtgagttg ttgtagtcca gcggctaaag ccattgagaa ccccgtagtt acctgttgca
      541 gtaccaaagc tgtagtaagt attaatccca taaaactagc caccaaagat gcgttgcaac
      601 agtttaaaga tgtactgcca tatcttttgt taggagtatt aataggatct tttatttatg
      661 gctttattcc ctcagcgtgg attgccgctc atgcaggagc agataatccc ttcgccatcc
      721 cattaagcgc cgttgtcggt attccgctgt atatccgggc tgaggcggtt atccctctgg
      781 catctgttct gatgacaaaa ggaatgggtc tgggagcatt aatggcatta atcatcggca
      841 gtgccggcgc aagcctgacg gaagtgatat tacttaaatc aatgttcaga atgccgatga
      901 tagctgcatt cctgacggtt atattaggta tggctatctt gatgggctat ttgactcaat
      961 tgctatttta aatagatgcc agcagtaaac tataagatgc atagttagtg ccatatcctt
     1021 ctaagtagaa aatcatagac aggataatcc ttaaaaatca caaatgattt aactaatgca
     1081 ttgagaatga tgacggctcg gtcaacaagt catcaacaga caataaccac gtaatcatgc
     1141 ttctctgaca ttttatcgga gaagcaatga ctctttgtct cagtaccctc ctccttttta
     1201 aaaggcagat ttcagtcgct aatacaacac atttttaaat acactttaac ctggttcatt
     1261 attacacatc taaaacatca caaaaatatt acttcacaca ctatcggaga acataataac
     1321 tttttaaaat gagcgttttt tgatagttgt taacgttttc ctttacgata ctatcactgc
     1381 ccttttttta tacacaaata ttaccgggtg gaaatgaaag ggaatttaaa ggtaattata
     1441 tcaggaagat ataacgcatt atatttattg tgtgtagaag aaaatccatt cctgttctaa
     1501 ttatccaatt taaaaacatt agcataaaac agatgatgaa taaggaatgt tatgtcaatt
     1561 gactttaccc caggtataat aaatacatat cacggcgata tttataactg cacaacaaat
     1621 accgataatg ttaaaacgcc agacacaccg aagtggcctt gtgataattg ggaagaacaa
     1681 caacccatta attccacatt ctctggagaa ggatacaacc ctgaacaatt tgatttagcg
     1741 caacaacaac tccaacaaat taatgcatgc cacacgaaca ccacatatac aaatgcagac
     1801 tactcaaaag ttgtggttca acttgtaagt cttattaata acattgaaac gataagttcg
     1861 acaccactta cgcaacagac acaatcaata cttaatcaga ttcataacat tagatacgaa
     1921 aaggataagg attctgtatg tcaaatcatt gttgtcgtta atccagaacc caacaatcca
     1981 atcataacta aaattctcgt agaagaagga atacccgaaa gattcagtgt ccaaaccgtt
     2041 tcttccgata ataaaaattt tgctgggcaa cgagctgact taccaactga tataagagac
     2101 attcaaagct tgtacttaaa aatggcgaag ctgtatattg agcacagtaa aaatgaaaat
     2161 aggatggaag cccttgctgg atgcgacttt attgatttca acatgacagg gcaagatatg
     2221 tccaagctag tcctaacctt atcaaaattt tattttgaag atctacttaa tataaatttc
     2281 actgacgcaa atttatttaa cactattttt tcacacaagg aaaatcccat ccccaaacta
     2341 cataaatatg aacaacatct tgacaaacaa atcaatggtt tgttttctac attgttaact
     2401 attaatgaca actctttacg cgcgaaagcg gaaattgctt cgagaataat tgatttctta
     2461 gaggcaaagg tagtgaatct ttcatttgat gacatattaa aatataagca agagttcaaa
     2521 aaaatatgtt ataaacaact tcaagagttt acaacaccct ctctatacaa taaaattcaa
     2581 aaatgggcaa caatgtctaa aaatgaattc atagcatttc attacgagac acttcaaccc
     2641 gaaaagattt catacccctt ttatttaaag agagatctgc ctaatgaaaa agatataaat
     2701 tatggggtgg aaatagaaat accatctggc aaaagaattc gcttatcaaa tcattatcag
     2761 aatataatac cataatattg attattagca ctttataatc attgaataaa aatagatttt
     2821 atgtaatttt aaaacaaagg gttatattat tgggcgatgg atactcatac cacgccccaa
     2881 aataacggaa ttgaactaaa cagaagggat ataaacatca gacaggttta cgttactatc
     2941 aggcatatca cctcagaatc agatgaaaac tataaagaaa tatctattat ggttttaata
     3001 tttgttgata aggatagtaa catgaacatg acaaaaggtg cactcatcct cagcctttca
     3061 tttttgcttg ccgcatgtag ttcaattccg caaaatatca aaggcaataa ccaacctgat
     3121 attcaaaaaa gttttgttgc tgttcataac cagccggggt tatatgttgg tcaacaagcg
     3181 cgctttggtg ggaaggttat caacgttatc aatggcaaaa cggatacgtt gttagaaatc
     3241 gctgtattac cgttggatag ctatgcgaag cctgatattg aagccaacta tcagggccga
     3301 ctgctcgcca gacaaagcgg cttccttgat ccagtgaact atcgtaatca ctttgttacc
     3361 atcctcggta ccattcaggg tgaacaacca ggctttatca ataaagtccc gtataacttc
     3421 ctggaagtga atatgcaggg catccaggtg tggcatttga gagaagtggt taataccacc
     3481 tataacctgt gggattacgg ctatggtgca ttctggccgg aaccgggctg gggtgcgcct
     3541 tactacacca atgcggtgag tcaggtaaca cctgagctgg tcaaataaca ccaccgaaag
     3601 atgcagatgc tttctcagca tctgcatcat gtattacata aaattaatat acagtaagct
     3661 aactattatt attataagcc ctgtcctgtt aattaccttt ggcaaactga ttataaagtt
     3721 aatgtccgca ccaggagtcg gttatgtttc ttataattac cagggatacg atgttcttca
     3781 ccgcgatgaa aaacattctg agtaaaggta atgtcgttca tatacagaac gaagaagaga
     3841 tcgacgtaat gttgcatcag aatgccttcg tcattattga tacattaatg aataatgtat
     3901 ttcattctaa ttttctcact caaattgaac gattaaaacc tgtccatgtc attgttttct
     3961 ccccctttaa tattaaacgc tgtctgggga aagtaccggt gacctttgtt ccgcggacta
     4021 tcactatcat tgattttgtc gcactcatca atggcagtta ctgctctgtg cctgaagcga
     4081 atgtgtcact ttcgcgcaag caacatcagg ttctgagctg cattgcgaat caaatgacaa
     4141 ctgaagatat tctggagaaa ctgaaaatat cgctaaaaac gttctactgc cataaacaca
     4201 atatcatgat gatcctcaat cttaagcgga tcaatgagct ggtacgccat cagcatattg
     4261 attatctggt gtgaatttca ggcttacggt gagtctggct acgctgccac acagattagc
     4321 taattgaaac gcctttcacc cctgccatac cttttaataa tcgcaacagg tcttctatca
     4381 acgttgtggc atgaagctgg atatcaatgg cgaccacttc atgatcttca ttttcctgca
     4441 acgaaactaa atcagttttt attttttgct gcttaaacca gtcgagcatt gataccgtat
     4501 taccgttcac taatgtcagc tgtaaatggt aatttttgtt catcaggcgg aaggttaatt
     4561 gatggaagac ttccagcacc aacaaggtca tcactgaacc ataaataccc agttcgtaca
     4621 taccgctgcc aataaccata cctatgcggc ggtcacccag atatccgctg ccgtcgtcag
     4681 acctacaata ttttggttac gaacgagaat gttacctgca ccgataaacc cgacgcccgt
     4741 caccacctga gcagcaatac ggctggggtc gagtccgacg tgatctaaag acagcacgtc
     4801 agcaaaacca tatttcgaaa caatcataaa cagggcgctt cccatgccaa ttaatacatg
     4861 tgtgcgta
//
LOCUS       DAJTQY010000187         4811 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.188, whole genome shotgun sequence.
ACCESSION   DAJTQY010000187 DAJTQY010000000
VERSION     DAJTQY010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4811
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.188"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            141..545
                     /locus_tag="OO349_003906"
     CDS             141..545
                     /locus_tag="OO349_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000512977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HCR5868802.1"
                     /translation="MGISLNHGVHAKVSTPVHLRKARTCYDHLAGEVAVKIYDSLCQQ
                     QWITENGSMITLSGIQYFHEMGIDVPSKHSRKICCACLDWSERRFHLGGYVGAALFSL
                     YESKGWLTRHLGYREVTITEKGYAAFKTHFHI"
     gene            complement(523..1146)
                     /gene="tetR(B)"
                     /locus_tag="OO349_003907"
     CDS             complement(523..1146)
                     /gene="tetR(B)"
                     /locus_tag="OO349_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HCR5868803.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            1228..2433
                     /gene="tet(B)"
                     /locus_tag="OO349_003908"
     CDS             1228..2433
                     /gene="tet(B)"
                     /locus_tag="OO349_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HCR5868804.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(2546..3139)
                     /gene="tetC"
                     /locus_tag="OO349_003909"
     CDS             complement(2546..3139)
                     /gene="tetC"
                     /locus_tag="OO349_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HCR5868805.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            3272..3643
                     /locus_tag="OO349_003910"
     CDS             3272..3643
                     /locus_tag="OO349_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5868806.1"
                     /translation="MQLRYDDKQFHTTVIKDVLLWIEHNLDQSLLLDDVANKAGYTKW
                     YFQRLFKKVTGVTLASYIRARRLTKAAVELRLTKKTILEIALKYQFDSQQSFTRRFKY
                     IFKVTPSYYRRNKLWELEAMH"
     gene            complement(3653..>4811)
                     /locus_tag="OO349_003911"
     CDS             complement(3653..>4811)
                     /locus_tag="OO349_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS4-like element ISVsa5 family transposase"
                     /protein_id="HCR5868807.1"
                     /translation="LHLKRLNSLTLACHALLDCKTLTLTELGRNLPTKARTKHNIKRI
                     DRLLGNRHLHKERLAVYRWHASFICSGNTMPIVLVDWSDIREQKRLMVLRASVALHGR
                     SVTLYEKAFPLSEQCSKKAHDQFLADLASILPSNTTPLIVSDAGFKVPWYKSVEKLGW
                     YWLSRVRGKVQYADLGAENWKPISNLHDMSSSHSKTLGYKRLTKSNPISCQILLYKSR
                     SKGRKNQRSTRTHCHHPSPKIYSASAKEPWVLATNLPVEIRTPKQLVNIYSKRMQIEE
                     TFRDLKSPAYGLGLRHSRTSSSERFDIMLLIALMLQLTCWLAGVHAQKQGWDKHFQAN
                     TVRNRNVLSTVRLGMEVLRHSGYTITREDLLVAATLLAQNLFTHGYALGKL"
BASE COUNT         1290 a          910 c         1034 g         1577 t
ORIGIN      
        1 tggcgaatat atcagcttca acggcgagca gtcatttatc taaattatta gattgccagc
       61 taatcacagt agtagctcaa ggcaagcatc gttattttcg gctagcagga aaagatattg
      121 ctgaattgat ggaaagtatg atggggatct ccttaaacca tggcgtacat gccaaagttt
      181 ccacgccagt gcatttacga aaagcacgta cttgctatga tcatttagct ggcgaagttg
      241 ccgttaagat ctatgattcc ctttgtcaac agcaatggat cactgaaaat ggttcaatga
      301 tcacattaag tggtattcaa tattttcatg aaatgggaat tgacgttcct tccaaacatt
      361 cacgtaaaat ctgttgtgcg tgtttagatt ggagtgaacg ccgtttccat ttaggtgggt
      421 acgttggagc cgcattattt tcgctttatg aatctaaagg gtggttaact cgacatcttg
      481 gttaccgtga agttaccatc acggaaaaag gttatgctgc ttttaagacc cactttcaca
      541 tttaagttgt ttttctaatc cgcatatgat caattcaagg ccgaataaga aggctggctc
      601 tgcaccttgg tgatcaaata attcgatagc ttgtcgtaat aatggcggca tactatcagt
      661 agtaggtgtt tccctttctt ctttagcgac ttgatgctct tgatcttcca atacgcaacc
      721 taaagtaaaa tgccccacag cgctgagtgc atataatgca ttctctagtg aaaaaccttg
      781 ttggcataaa aaggctaatt gattttcgag agtttcatac tgtttttctg taggccgtgt
      841 acctaaatgt acttttgctc catcgcgatg acttagtaaa gcacatctaa aacttttagc
      901 gttattacgt aaaaaatctt gccagctttc cccttctaaa gggcaaaagt gagtatggtg
      961 cctatctaac atctcaatgg ctaaggcgtc gagcaaagcc cgcttatttt ttacatgcca
     1021 atacaatgta ggctgctcta cacctagctt ctgggcgagt ttacgggttg ttaaaccttc
     1081 gattccgacc tcattaagca gctctaatgc gctgttaatc actttacttt tatctaatct
     1141 agacatcatt aattcctaat ttttgttgac actctatcat tgatagagtt attttaccac
     1201 tccctatcag tgatagagaa aagtgaaatg aatagttcga caaagatcgc attggtaatt
     1261 acgttactcg atgccatggg gattggcctt atcatgccag tcttgccaac gttattacgt
     1321 gaatttattg cttcggaaga tatcgctaac cactttggcg tattgcttgc actttatgcg
     1381 ttaatgcagg ttatctttgc tccttggctt ggaaaaatgt ctgaccgatt tggtcggcgc
     1441 ccagtgctgt tgttgtcatt aataggcgca tcgctggatt acttattgct ggctttttca
     1501 agtgcgcttt ggatgctgta tttaggccgt ttgctttcag ggatcacagg agctactggg
     1561 gctgtcgcgg catcggtcat tgccgatacc acctcagctt ctcaacgcgt gaagtggttc
     1621 ggttggttag gggcaagttt tgggcttggt ttaatagcgg ggcctattat tggtggtttt
     1681 gcaggagaga tttcaccgca tagtcccttt tttatcgctg cgttgctaaa tattgtcgct
     1741 ttccttgtgg ttatgttttg gttccgtgaa accaaaaata cacgtgataa tacagatacc
     1801 gaagtagggg ttgagacgca atcgaattcg gtatacatca ctttatttaa aacgatgccc
     1861 attttgttga ttatttattt ttcagcgcaa ttgataggcc aaattcccgc aacggtgtgg
     1921 gtgctattta ccgaaaatcg ttttggatgg aatagcatga tggttggctt ttcattagcg
     1981 ggtcttggtc ttttacactc agtattccaa gcctttgtgg caggaagaat agccactaaa
     2041 tggggcgaaa aaacggcagt actgctcgga tttattgcag atagtagtgc atttgccttt
     2101 ttagcgttta tatctgaagg ttggttagtt ttccctgttt taattttatt ggctggtggt
     2161 gggatcgctt tacctgcatt acagggagtg atgtctatcc aaacaaagag tcatcagcaa
     2221 ggtgctttac agggattatt ggtgagcctt accaatgcaa ccggtgttat tggcccatta
     2281 ctgtttgctg ttatttataa tcattcacta ccaatttggg atggctggat ttggattatt
     2341 ggtttagcgt tttactgtat tattatcctg ctatcgatga ccttcatgtt aacccctcaa
     2401 gctcagggga gtaaacagga gacaagtgct tagttatttc gtcaccaaat gatgttattc
     2461 cgcgaaatat aatgaccctc ttgataaccc aagagggcat tttttacgat aaagaagatt
     2521 tagcttcaaa taaaacctat ctattttatt tatctttcaa gctcaataaa aagccgcggt
     2581 aaatagcaat aaattggcct tttttatcgg caagctcttt taggtttttc gcatgtattg
     2641 cgatatgcat aaaccagcca ttgagtaagt ttttaagcac atcatcatca taagctttaa
     2701 gttggttctc ttggatcaat ttgctgacaa tggcgtttac cttaccagta atgtattcaa
     2761 ggctaatttt ttcaagttca ttccaaccaa tgataggcat cacttcttgg atagggataa
     2821 ggtttttatt attatcaata atataatcaa gataatgttc aaatatactt tctaaggcag
     2881 accaaccatt tgttaaatca gtttttgttg tgatgtaggc atcaatcata attaattgct
     2941 gcttataaca ggcactgagt aattgttttt tatttttaaa gtgatgataa aaggcacctt
     3001 tggtcaccaa cgcttttccc gagatctcat ctattgaaac agcttgatag cctttttcaa
     3061 caaacaatat tcgtgctgag ttaaccagtg attgataggt actcttaaaa ttttcttgtt
     3121 gatgattttt attttccatg atagatttaa aataacatac cgtcagtatg tttatggtat
     3181 catgatgatg tggtcgtgac aatcttaaga acatttaggt tattttatgt atattgaaca
     3241 gcattctcgc tatcaaaata aagctaataa catccaatta agatatgatg ataagcagtt
     3301 tcatacaacg gttatcaaag atgttctatt atggattgaa cataatttag atcagtcttt
     3361 actgcttgat gatgtggcga ataaagcggg ttataccaag tggtattttc agcggctgtt
     3421 caaaaaagta acaggggtca cactggctag ctatattcgt gctcgtcgtt tgacgaaagc
     3481 ggctgttgag ttgaggttga cgaaaaaaac tatccttgag atcgcattaa aatatcaatt
     3541 tgattcccaa caatctttta cacgtcgatt taagtacatt tttaaggtta caccaagtta
     3601 ttatcggcgt aataaattat gggaattgga ggcaatgcac tgagagatcc cctcataatt
     3661 tccccaaagc gtaaccatgt gtgaataaat tttgagctag tagggttgca gccacgagta
     3721 agtcttccct tgttattgtg tagccagaat gccgcaaaac ttccatgcct aagcgaactg
     3781 ttgagagtac gtttcgattt ctgactgtgt tagcctggaa gtgcttgtcc caaccttgtt
     3841 tctgagcatg aacgcccgca agccaacatg ttagttgaag catcagggcg attagcagca
     3901 tgatatcaaa acgctctgag ctgctcgttc ggctatggcg taggcctagt ccgtaggcag
     3961 gacttttcaa gtctcggaag gtttcttcaa tctgcattcg cttcgaatag atattaacaa
     4021 gttgtttggg tgttcgaatt tcaacaggta agttagttgc tagaacccat ggctcctttg
     4081 ccgacgctga gtagatttta ggtgacgggt ggtgacaatg agtccgtgtc gagcgctgat
     4141 tttttcggcc tttagagcga gatttataca atagaatttg gcatgagatt ggattgcttt
     4201 tagtcagcct cttatagcct aaagtctttg agtgactaga tgacatatca tgtaagttgc
     4261 tgataggttt ccagttttcc gctcctaggt ctgcatattg tacttttcct cttactcgac
     4321 ttaaccagta ccaacccagc ttctcaacgg atttatacca tggcacttta aagccagcat
     4381 cactgacaat gagcggtgtg gtgttactcg gtagaatgct cgcaaggtcg gctagaaatt
     4441 ggtcatgagc tttctttgaa cattgctctg aaagcgggaa cgctttctca taaagagtaa
     4501 cagaacgacc gtgtagtgcg actgaagctc gcaataccat aagtcgtttt tgctcacgaa
     4561 tatcagacca gtcaacaagt acaatgggca tcgtattgcc cgaacagata aagctagcat
     4621 gccaacggta tacagcgagt cgctctttgt ggaggtgacg attacctaac aatcggtcga
     4681 ttcgtttgat gttatgtttt gttctcgctt tggttggcag gttacggcca agttcggtaa
     4741 gagtgagagt tttacagtca agtaatgcgt ggcaagccaa cgttaagctg ttgagtcgtt
     4801 ttaagtgtaa t
//
LOCUS       DAJTQY010000188         4716 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.189, whole genome shotgun sequence.
ACCESSION   DAJTQY010000188 DAJTQY010000000
VERSION     DAJTQY010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4716
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.189"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            <1..282
                     /locus_tag="OO349_003912"
     CDS             <1..282
                     /locus_tag="OO349_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5868808.1"
                     /translation="SSEQKLSFYLSGATTDSSRQVFANTAPDATKASGVGVTLMRNGK
                     ILATGENVSLGTVNKSKVPLGLSATYGQTGNKVSAGTVQSVIGVTFIYE"
     gene            616..2895
                     /gene="ydeP"
                     /locus_tag="OO349_003913"
     CDS             616..2895
                     /gene="ydeP"
                     /locus_tag="OO349_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HCR5868809.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRHDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFPPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            3143..3346
                     /gene="safA"
                     /locus_tag="OO349_003914"
     CDS             3143..3346
                     /gene="safA"
                     /locus_tag="OO349_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HCR5868810.1"
                     /translation="MHATTVKNKITQRDNYKEIMSAIVVVLLLTLTLIAIFSAIDQLS
                     ISEMGRIARDLTHFIINSLQDWK"
     gene            3414..4175
                     /gene="ydeO"
                     /locus_tag="OO349_003915"
     CDS             3414..4175
                     /gene="ydeO"
                     /locus_tag="OO349_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HCR5868811.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFNENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQRHTGMNTGNTMSALAI"
     gene            4577..>4716
                     /locus_tag="OO349_003916"
     CDS             4577..>4716
                     /locus_tag="OO349_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HCR5868812.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SD"
BASE COUNT         1353 a         1004 c         1046 g         1313 t
ORIGIN      
        1 tcaagcgaac aaaagctctc attttatctt tcgggtgcca ccactgacag tagccgacaa
       61 gtgtttgcta acacagcacc tgatgcaaca aaagcatcgg gagtgggtgt cactctaatg
      121 cgtaatggaa aaatactggc tacgggagaa aatgtctccc tggggacagt aaataaaagc
      181 aaagtacctc tgggtttaag cgcaacctat ggacaaacag gaaacaaggt ttcagccgga
      241 accgtccagt cagtcattgg tgtcactttt atctatgaat aattgtgatt ttgaccttag
      301 tctatgcgct ccattcacga aaattgcgcg gataattatt ttgtgaaggc tattatccta
      361 cacctgtaag aaaatccgcg cacaattgaa gccagagtct tattttttat attgaaaaat
      421 atgttaattt aaacactatg agagatgatg cttaacttat cgtatttaat ctattgttta
      481 acgataatag attaaaaata gaaagagagt agtcccttac tcttatatat ccatgttggc
      541 gataatccct ttgtatgtac tgtgcatcat cgctattaca aatcctaata attcatttcc
      601 acacaggata agtagatgaa gaaaaaaatt gaatcctacc agggtgctgc aggtggttgg
      661 ggtgctgtta aatccgtagc gaatgcagta cgtaagcaga tggatatacg ccatgatgtt
      721 attgccatgt ttgacatgaa taagccagag ggctttgact gtccgggttg tgcatggcca
      781 gatcctaagc acagtgcgtc attcgacatt tgtgaaaacg gcgcaaaagc aatcgcctgg
      841 gaagtcacgg ataagcaggt aaatgcctct ttctttgctg agaatacggt tcaatcatta
      901 cttacctggg gagatcacga gcttgaggct gcggggcgtc tcactcagcc tttgaaatat
      961 gatgccgtca gcgactgtta caagccatta agctggcaac aagctttcga cgaaattggc
     1021 gcacgccttc aaagctatag tgatcccaat caggttgaat tctatacttc gggccgcact
     1081 tccaatgaag ctgcctttct ttatcagctt tttgcccgtg aatacgggag caataacttt
     1141 cccgactgct ccaacatgtg ccatgaaccg acaagcgtgg gtttggcagc gagtatcggt
     1201 gtaggtaaag ggaccgtgtt gctggaagac tttgagaagt gcgatttagt catttgcatt
     1261 gggcataacc ctggtacaaa ccaccctcgc atgctgactt cgttgcgcgc tttagtgaaa
     1321 cggggagcga aaatgatcgc catcaatcct ctacaggaac gtggcctgga gcgatttacc
     1381 gcaccgcaaa acccgtttga aatgctgacg aactctgaga ctcagttggc cagtgcctac
     1441 tacaacgtgc gcattggtgg cgatatggcg ttgctcaagg ggatgatgcg cctgttaatt
     1501 gagcgcgatg atgctgcaag tgccgcaggt cggccctcat tgcttgatga cgaatttatt
     1561 caaacgcata ccgtcggctt tgacgagcta cgccgtgacg ttctcaattc cgagtggaaa
     1621 gatatcgaac gtatttctgg actaagtcag acacaaatcg ccgaactggc tgacgcatat
     1681 gccgctgccg aacgcaccat tatctgttac ggaatgggga tcactcagca cgaacatggt
     1741 acccagaacg tgcagcaact ggtcaatttg ctgttgatga aaggtaacat tggcaagcct
     1801 ggtgcgggta tctgcccact acgtggtcac tctaatgtac agggcgaccg aaccgtcggt
     1861 atcaccgaga aaccgtctgc agagtttctg gctcgcctgg gtgagcgcta tggcttcccc
     1921 ccacctcatg cacctggaca tgctgcaatt gccagcatgc aagcaatatg tacggggcag
     1981 gctcgagcat tgatctgcat ggggggcaac tttgcactgg caatgccaga tcgggaagcg
     2041 agcgctgtac cgttaacgca attagatttg gcggtacacg tagccactaa gcttaaccgc
     2101 tctcatctgc tgaccgcacg gcatagctat attctgccgg ttctggggcg tagcgagatt
     2161 gatatgcaaa aaagcggtgc gcaggcggta accgttgagg attcaatgtc gatgattcat
     2221 gcctcgcgtg gcgtgttaaa acccgccggt gtaatgctga aatcagagtg tgcagtggtc
     2281 gcgggaatcg cgcaggcagc actaccccag agcgtggtag cctgggagta tctggtggaa
     2341 gattatgatc gcattcgcaa tgacattgaa gctgtgctgc cagagttcgc cgactataac
     2401 cagcgcatcc gtcatcccgg tggttttcac ctgataaatg cagctgctga aaggcgctgg
     2461 atgacgccgt caggtaaggc taatttcatt accagcaaag ggctgttaga agatccctct
     2521 tcagcgttta acagtaagct ggtcatggcg acagtacgca gccacgatca gtacaacacg
     2581 acgatttatg gtatggatga tcgctatcga ggggtattcg gtcaacgaga tgtggtcttt
     2641 atgagtgcta aacaagctaa aatttgccgt gtaaaaaacg gcgaaagagt taatcttatt
     2701 gcgcttacgc cagacggtaa gcgcagctca cgccgcatgg atagattaaa agtggtcatt
     2761 taccctatgg ctgaccgctc actggtgacc tattttccag aatcgaatca catgctaaca
     2821 cttgataacc acgatccatt aagtggcatt cctggctata aaagtattcc ggttgaatta
     2881 gaaccatcaa attaatgcct cttctcattt cttctgctgt catccgcaca gcagaagaat
     2941 tcctcattga ctattattgc gcaatttgct cacatggatt aaattaaact acatactata
     3001 agatataaac ttctgcctac agctgtaaga aactccgctc agtactgaag caccagtcct
     3061 atttcttctt ttctccagcc tgttatatta agcatactga ttaacgactt ttaacgttat
     3121 ccgctaaata aacatatttg aaatgcatgc gaccacagtg aaaaacaaaa tcacgcaaag
     3181 agataactat aaagaaatca tgtctgcaat tgtggttgtc ttattactga cacttacgtt
     3241 gatagccatt ttttcggcaa ttgatcagct gagtatttca gaaatgggtc gcattgcaag
     3301 agatcttaca catttcatta tcaatagttt gcaagactgg aaataaacag caaattataa
     3361 atgtgaaatg tttaaaaggt atcgataaaa ctttattgtt ttaaggagat aaaatgtcgc
     3421 tcgtttgttc tgttatattt attcatcatg ctttcaacgc taacatttta gataaagatt
     3481 acgccttctc cgacggcgag atcctgatgg tagataacgc tgttcgtaca cattttgaac
     3541 cttatgagcg gcattttaaa gaaatcggat ttaatgaaaa taccattaaa aaatatctac
     3601 aatgcactaa catccagaca gtgacggtgc ctgttcctgc gaagttttta cgtgcttcaa
     3661 atgtaccgac tggattgctt aatgaaatga ttgcttatct caactcggaa gaacgcaatc
     3721 atcataattt ttcagaactt ttgctttttt cttgcctgtc tatttttgcc gcatgcaaag
     3781 gtttcattac actattaact aacggtgtgc tatccgtttc cgggaaagtg agaaatattg
     3841 tcaacatgaa gctggcgcac ccatggaagc tgaaagatat ttgtgactgc ctgtacatca
     3901 gtgaaagcct gttgaagaaa aaactaaagc aagagcaaac gacattctca cagattctat
     3961 tagatgccag aatgcagcac gcaaaaaatt tgatacgcgt agaaggttca gtcaataaaa
     4021 ttgccgaaca atgtggttat gccagtacat cttattttat ttatgcgttc cgcaaacatt
     4081 tcggcaacag tccgaagaga gtttctaagg agtaccgttg tcaaaggcac acgggtatga
     4141 atacgggcaa cacgatgagt gctttagcta tttgattatt tgctaacgag tagtcaacca
     4201 cgcacgctac gtaagaatta tatggggcag ccattccctg ccccgcgttg tttttaggcg
     4261 atatatttat tgaaataaat aagtgacatc catcacatat ttatgcactt gcataacctg
     4321 ttgcatgatt atttatgatc tcaattcggc attttgtcag taaaatgcaa taatttatta
     4381 aatatcaata aattagttgt ttatcggcga gaaattactt aatagaacag aaagtaatgt
     4441 caacgcttta tggactgtta tttccctttt tttagctaaa tctgctatct ctttatgtga
     4501 ctaacttcac ttacatccac ttatttctct tcgtaaaatt acttcggaat taagtacaat
     4561 aagaagagga acatttatga agtctgcatt aaagaaaagt gtcgtaagta cctcgatatc
     4621 tttgatactg gcatctggta tggctgcatt tgctgctcat gcggcagatg atgtaaagct
     4681 gaaagcaacc aaaacaaacg ttgctttctc agactt
//
LOCUS       DAJTQY010000189         4636 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.190, whole genome shotgun sequence.
ACCESSION   DAJTQY010000189 DAJTQY010000000
VERSION     DAJTQY010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4636
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.190"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            <1..311
                     /locus_tag="OO349_003917"
     CDS             <1..311
                     /locus_tag="OO349_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Involved in anaerobic NO protection; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HCR5868813.1"
                     /translation="LDNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQ
                     YYNQLHQLPLVALPLPFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA
                     "
     gene            468..1217
                     /locus_tag="OO349_003918"
     CDS             468..1217
                     /locus_tag="OO349_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HCR5868814.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            1239..1805
                     /gene="yieF"
                     /locus_tag="OO349_003919"
                     /gene_synonym="chrR"
     CDS             1239..1805
                     /gene="yieF"
                     /locus_tag="OO349_003919"
                     /gene_synonym="chrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HCR5868815.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(1859..3196)
                     /gene="adeP"
                     /locus_tag="OO349_003920"
     CDS             complement(1859..3196)
                     /gene="adeP"
                     /locus_tag="OO349_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HCR5868816.1"
                     /translation="MSHQHTTQTSGQRMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANSETLMSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            3361..4017
                     /gene="yieH"
                     /locus_tag="OO349_003921"
     CDS             3361..4017
                     /gene="yieH"
                     /locus_tag="OO349_003921"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HCR5868817.1"
                     /translation="MSRIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTEAEHVYRAEVARLFDSAIEGAGALLSAITAPMC
                     VVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVENCI
                     LVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWDITA
                     "
     gene            4084..4557
                     /locus_tag="OO349_003922"
     CDS             4084..4557
                     /locus_tag="OO349_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CbrB"
                     /protein_id="HCR5868818.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLVIIQ
                     VLPLFLLLSITTGAIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELFENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
BASE COUNT         1238 a         1162 c         1138 g         1098 t
ORIGIN      
        1 cgctggacaa tgtgttacag gagctggggc gcgaacgcac gattgctatg agcctgccgg
       61 aattcgagca gtcactgttt atggcagcgc aacccgacaa tctgctactg gcgaccgcgc
      121 cgcgctactg tcagtactac aatcaactcc atcaactgcc gttggttgct cttcctctcc
      181 cgtttgacga aagccagcaa aaaaagctgg aagttccttt taccctgctg tggcataaac
      241 ggaacagcca taatccgaag atcgtctggt tacgggaaac cattaaaaac ctttacgcgt
      301 cgatggcata accgaatcgt atgaaacgcg acccaatttc acaataaaat gtaaaaaagt
      361 tgtaataagc ttgtctgaat cgaactttta gccgctttag tctgtccatc attccagtaa
      421 atgattactc ttgtattcat aatggaccat taagcatgga gcgaaaaatg gcgactcact
      481 ttgcccgagg gattttaacg gaaggacatc tgatttctgt tcgtctcccc tcccagtgtc
      541 atcaagaagc ccgaaacatt ccccctcatc gtcaaagccg ttttctggcg tccagaggtt
      601 tactcgcaga actgatgttc atgctgtatg gcattggcga attgccggaa atcgtcaccc
      661 tgccgaaagg taaaccggtt ttcagtgata aaaatttgcc ttcgttttct atttcctatg
      721 ccgggaatat ggttggcgtg gcgttaacaa ccgaaggtga atgtggcctc gatatggaac
      781 tacagcgtgc gacgcgcggg tttcatagcc cacacgcgcc cgataaccac accttttcca
      841 gcaatgaatc gctatggatc agtaaacaaa acgatcctaa cgaagcgcgg gcgcagctca
      901 ttacgctgcg ccgaagcgtg ctaaaactaa ccggtgatgt tttgaatgac gatccgcgcg
      961 atctgcagct gctgcccatt gccggacgcc tgaaatgtgc tcatgtaaat catgtagaag
     1021 cgttatgcga cgcggaggac gtgctggtgt ggtccgtggc ggtcacgccc gcgattgaaa
     1081 agctcagtgt ctgggagtta gatggcaaac acagctggaa aagcctgccg gatattcaca
     1141 gccgcgccaa caatcctacc agccggatga tgcgttttgc ccagctctct accgtgaagt
     1201 ctttttcgcc aaattgatag acaaccacag gagtcattat gtctgaaaaa ttgcaggtgg
     1261 ttacgttact ggggagcctg cgcaaaggct catttaatgg catggttgca cgtaccctgc
     1321 cgaaaattgc tccggcgagc atggaagtca atgcgttacc ttccattgcc gacattcccc
     1381 tgtatgacgc tgacgtacag caggaagaag gttttccagc aacggttgaa gctctggcgg
     1441 aacagatccg tcaggctgac ggtgtggtga tcgtcacgcc ggaatataac tactcggtac
     1501 cgggtgggct gaaaaatgcc atcgactggc tttcccgcct gccggatcaa ccgctggccg
     1561 gtaaaccggt gttgattcag accagctcaa tgggcgtgat tggcggcgcg cgctgtcagt
     1621 atcacctgcg ccagattctg gttttcctcg atgcaatggt gatgaacaag ccggaattta
     1681 tgggcggcgt gattcagaac aaagttgatc cgcaaaccgg agaagtgatt gatcagggta
     1741 cgctggacca cctgaccggg caattgaccg catttggtga gtttattcag cgagttaaga
     1801 tctaaataaa aaacccgcca gtaatcatgc atggcgggtt tttaatgcgc tatcgatttt
     1861 aatgagcgtc gataaataca atcttcagga taaacagcag cgcaacgatg attacgcacg
     1921 ggctaaggtc acgcagacgc ccggtaccaa tcttcatcac gcagtaggag ataaagccca
     1981 gcgcaatacc ttcggtaatc gaaaagctga acggcatcat cacggcggta ataaacgccg
     2041 gaacagattc agtaagatcc tgccagttca cgcgagcaag acttgaggtc atcaacacgc
     2101 caacgtagat caacgcgcca gctgcagcgt agcctggcac cattcccgcc agcggcgaca
     2161 gaaagataac cagcaggaac agcagaccaa caaccactgc cgtcagaccg gtacgaccgc
     2221 caaccgatac gccggaagag gactcaatat aagccgtaac ggaagaagta ccgataaacg
     2281 aaccggtcac ggaagagata ctgtcgacat acagcgcctg cttcatgcgc gggaatttcc
     2341 ccttctcatc cgccagacct gctttatcgg tcacgccaat cagcgtaccg gaggagtcaa
     2401 acaagttgac caacatgaaa gagaaaatca cccctgccag cccgaggtta aacgacccgg
     2461 ctaaatctac atgacccaca actgtcatta cgctcggcgg cgcagaaacg atgccattgt
     2521 agtgcacatc acccagcatc cagcccagca gcgtcgtcac cacgatagaa accagcaccg
     2581 ctgcgtgaat gttgcgcgag gccagaatag caatgatgaa aaagccgagg atacccagaa
     2641 gtacgctgtg agaagtcaga ttaccgatgc tcatcagcgt ttccgagtta gcgacaatca
     2701 cacctgcgtt tttcagcccc atcatgccga taaacaggcc gataccgctg gtaataccca
     2761 cacgcagact taccggaatg ttagcaatca tccagtaacg aacgcggaaa atggtcaaaa
     2821 gaagcagtcc gacagcgccc cagaagattg cgcccatacc gatctgccac ggcagcccca
     2881 ttgcctggac aaccacaaaa gcgaaaaagg cgttaagccc catcgcagga gccagcgcca
     2941 ccggaaggtt tgcaaacaat cccatcataa tgctaccgaa agcagcaatc agacaggttg
     3001 ttacgaagac ggcactggtg tccatgccag caacgcccag aatttgtggg ttaacaaata
     3061 cgatgtagac catcgtcagg aaggtggtga acccggcaat cacttcagtt cgtgccgtag
     3121 taccatgttc gcgcagttta aaaacacgtt ccagcatcct ctgaccagaa gtctgggtgg
     3181 tatgttgatg actcattatc tatttccgaa caaggaggga aaatccgtcg ctatcgtata
     3241 ccaaaatgcg acaataggcg cgtttgtgag agactttttt attggattta cttatacggc
     3301 aacgattgcg ttgcgcaaat cggctctacg aaaacgttaa actgattaaa aaggaaaggc
     3361 atgtcccgga tagaagcggt atttttcgac tgcgacggta cgctggtcga cagtgaagtc
     3421 atttgctccc gcgcatatgt aacgatgttt caggaatttg gtattacgct cgatcctgaa
     3481 gaggtattca aacgtttcaa aggtgtaaaa ctgtacgaaa ttatcgatat tgtttccctt
     3541 gaacatggtg ttacgttagc gaaaacagaa gctgaacacg tttaccgtgc agaagtcgct
     3601 cggctgttcg attcagccat cgaaggggct ggagcgctcc tgtcagcgat cactgcgcca
     3661 atgtgtgtgg tatctaacgg cccaaataac aaaatgcagc attctatggg caagctgaat
     3721 atgttgcact acttcccgga taaactgttc agcggctacg atattcagcg ctggaagcca
     3781 gacccggcgt taatgttcca tgcggcaaaa gcgatgaatg taaatgtaga aaactgcatt
     3841 ctggttgatg actcagttgc cggtgcgcaa tctggtatcg acgcaggtat ggaagtgttc
     3901 tacttctgcg ccgacccgca caataagccg atcgttcacc cgaaagtcac cacctttacc
     3961 catctttcgc agttaccgga actgtggaaa gcgcgtggtt gggatattac ggcatagttc
     4021 ttcacactcc cttcacttac cccgcttaaa ttggcgctca aaggtaagta aagggagttt
     4081 gatatgtctg tttcacgtcg ggtaatacat cacggacttt attttgcggt tttaggaccg
     4141 ttaattggtg ttctgtttct tgtcctctac atattcttcg caaaagaacc gctggttctt
     4201 ttggtaataa tacaggtact tccattgttt ctcttattgt cgattactac gggagctatt
     4261 cctgcattgt taaccggcgt gatggtcgcc tgtctgccgg aaaagatcgg gtcacagaaa
     4321 aattatcgtt gtctggctgg tggcataggt ggcgtcgtta tcaccgagat ctattgtgca
     4381 gttattgtac atattaaggg catggcttcc tcggagttgt ttgaaaacat tctttctggt
     4441 gacagtctcg ttgtccgcat cattcctgca ttgctggcag gtgtggtgat gagcagaatc
     4501 attacccgtc tgcccggatt ggatatttca tgtcctgaaa cagactcttt aagttaagcg
     4561 ggatacttta tctttgggct actcaaaagc agacaggatg ttactatgac tcaaaatatc
     4621 aggccgttac cccaat
//
LOCUS       DAJTQY010000190         4611 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.192, whole genome shotgun sequence.
ACCESSION   DAJTQY010000190 DAJTQY010000000
VERSION     DAJTQY010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4611
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.192"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            225..2648
                     /gene="pgaA"
                     /locus_tag="OO349_003923"
                     /pseudo
     CDS             225..2648
                     /gene="pgaA"
                     /locus_tag="OO349_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309297.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine export
                     porin PgaA"
     gene            2657..4150
                     /gene="pgaB"
                     /locus_tag="OO349_003924"
     CDS             2657..4150
                     /gene="pgaB"
                     /locus_tag="OO349_003924"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000945570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase PgaB"
                     /protein_id="HCR5868819.1"
                     /translation="MLRNGNKYLLMLVSIIMLTACISQSRTSFIPPQDRESLLAEQPW
                     PHNGFVAISWHNVEDEAADQRFMSVRTSALREQFAWLRENGYQPVSIAQIREAHRGGK
                     PLPEKAVVLTFDDGYQSFYTRVFPILQAFQWPAVWAPVGSWVDTPVDEQVKFGDEMVD
                     REYFATWQQVREVARSRLVEVASHTWNSHYGIQANSTGSLMPVYVNRAYFTDHARYET
                     AAEYRERIRLDAVKMTEYLRTKAEVNPHVFVWPYGEANGIAIEELKKLGYDMFFTLES
                     GLANASQLDSIPRVLIANNPSLKEFAQKIITVQEKSPQRIMHIDLDYVYDENRQQMDR
                     NIDVLIQRVKDMQISTVYLQAFADPDGDGLVKEVWFPNHLLPMKADIFSRVAWQLRTR
                     SGVNIYAWMPVLSWDLDPTLTRVKYLPTGEKKAQIHPEQYHRLSPFDDRVRAQVGMLY
                     EDLAGHAAFDGILFHDDALLSDYEDASAPAITAYQQAGFSGSLSEIR"
     gene            4154..4611
                     /gene="pgaB"
                     /locus_tag="OO349_003925"
                     /pseudo
     CDS             4154..4611
                     /gene="pgaB"
                     /locus_tag="OO349_003925"
                     /inference="COORDINATES: protein motif:HMM:NF026233.1"
                     /note="in Yersinia this protein is involved in biofilm
                     formation and hemin adsorption; related protein PgaB in
                     Escherichia coli is an outer membrane N-deacetylase
                     involved in poly-beta-1,6-N-acetyl-D-glucosamine (PGA)
                     export; incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase"
BASE COUNT         1318 a         1033 c         1061 g         1199 t
ORIGIN      
        1 atttatgccg tattcctgaa gatcctcatc attggaatgg attttcgggc gagaaaagga
       61 ttttatatgg acactctgct catcatttct tcttctcatc atcaacaatt cacgtctctc
      121 ttccgcgttt aataacggat tatgaggtgc aaaaatatct ttcttttcag ttacctgtaa
      181 ttagatacag agagagattt tggcaataca tggagtaata caggatgtat tcaagtagca
      241 gaaaaaggtg cccgaaaacc aaatgggctt tgaaacttct tactgccgca tttttagcag
      301 cgagtcccgc ggcgaagagt gctgttaata acgcctatga tgcattgatt attgaagctc
      361 gcaagggtaa tactcagcca gctttgtcat ggtttgcact aaaatcagca ctcagcaata
      421 accaaattgc tgactggtta cagattgcct tatgggccgg gcaagataaa caggttatta
      481 ccgtttacaa ccgctaccgt catcagcaat taccagcgcg tggttatgca gctgtcgccg
      541 tcgcttatcg taacctgcaa caatggcaaa actcgcttac actgtggcaa aaggcgctct
      601 ctctggagcc gcaaaataag gattatcaac ggggacaaat tttaaccctg gcagatgcgg
      661 gtcactatga tactgcgctg gttaaactta agcagcttaa ctctggagca ccggacaaag
      721 ccaatttact cacagaagcc tatatctata aactggcggg gcgtcatcag gatgaattac
      781 gggcgatgac agagtcatta cctgaaaatg catctacgca acaatatccc acagaatacg
      841 tgcaggcatt acgtaataat caacttgctg ccgcgattga cgatgccaat ttaacgccag
      901 atattcgcgc tgatattcat gccgaactgg tcagactgtc gtttatgcct acgcgcagtg
      961 aaagtgaacg ttatgccatt gccaatcgcg ccctcgccca atacgctgca ttagaaattc
     1021 tgtggcacga taacccagac cgcactgccc agtaccagcg tattcaggtt gatcatcttg
     1081 gcgcgttatt aactcgcgat cgttataaag acgtcatttc tcactatcag cgattaaaaa
     1141 agacggggca aattattccg ccctaggggc aatattgggt tgcatcggct tatctcaaag
     1201 atcatcagcc gaaaaaagca cagtcaataa tgaccgagct cttttatcac aaggagacca
     1261 ttgccccgga tttatccgat gaagaacttg cggatctctt ttacagccac ctagagagtg
     1321 aaaattatcc gggcgcgcta actgtcaccc aacataccat taatacttcg ccgcctttcc
     1381 ttcggttaat gggcacgcct acgagcatcc cgaatgatac ctagttacag gggcattcgt
     1441 ttctctcaac cgtagcaaaa tatagtaatg atcttcctca ggctgaaatg acagccagag
     1501 agcttgctta taacgcacca ggaaatcagg gactgcgcat tgattacgcg agtgtgttac
     1561 aagcccgcgg ttggcctcgt gcagcagaaa atgaattaaa aaaagcagaa gtgatcgagc
     1621 cacgtaatat taatctggag gttgaacaag cctagacagc attaacgtta caagaatggc
     1681 agcaggcagc tgtcttaacg cacgatgttg tcgaacgtga accgcaagat cccggcgttg
     1741 tacgtttaaa acgtgcggtt gatgtacata atcttgcaga gcttcgtata gctggttcaa
     1801 caggaattga tgccgaaggc ccggatagtg gtaaacatga tgtcgactta accaccatcg
     1861 tttattcacc actgctgaag gataactggc gcggttttgc tggattcggt tatgccgatg
     1921 gacaatttag cgaaggaaaa gggattgttc gcgactggct ggcgggtgtt gagtggcggt
     1981 cacgtaatat ctggctcgag gcggagtacg ctgaacactt tttcaatcat gagcataaac
     2041 ccggcgcgcg cctgtctggc tggtatgatt ttaatgataa ctggcgtatt ggttcgcaac
     2101 tggaacgcct ctctcaccgc gttccattac gggcaatgaa aaatggtgtt acaggcaaca
     2161 gtgctcaggc ttatgttcgc tggtatcaaa atgagcggcg taagtacggt gtctcctggg
     2221 ctttcactga tttttccgac agtaaccagc gtcatgaagt ctcaattgag ggtcaggaac
     2281 gcatctggtc ttcaccatat ttgattgtcg atttcctacc cagtctgtat tacgaacaaa
     2341 atacagaaca cgatacccca tactacaacc ctataaaaac gttcgatatt gttccggcat
     2401 ttgaggcaag tcatttgtta tggcgaagct atgaaaatag ctgggagcaa atcttcagcg
     2461 caggtgttgg tgcctcctgg caaaaacatt atggcacgga tgtcgtcacc caactcggct
     2521 acgggcaacg cattagctgg aatgacgtga ttgatgctgg cgcaacgcta cgctgggaaa
     2581 aacgacctta tgacggtgac agagaacaca acttatacgt tgaattcgat atgacattca
     2641 gattttaagg ataaatatgt tacgtaatgg aaataaatat ctcctgatgc tggtgagtat
     2701 aattatgctc accgcgtgca tcagccagtc aagaacatca tttataccgc cacaggatcg
     2761 cgaatcttta ctcgccgagc aaccgtggcc gcataatggt tttgtggcga tttcatggca
     2821 taacgttgaa gacgaagctg ccgaccagcg ttttatgtca gtgcggacat cagcactgcg
     2881 tgaacaattt gcctggctgc gcgagaacgg ttatcaaccg gtcagtattg ctcaaattcg
     2941 tgaagcacat cgaggaggaa aaccgctacc ggaaaaagca gtagtgctga cttttgatga
     3001 cggctaccag agtttttata cccgcgtctt cccaattctt caggccttcc agtggcctgc
     3061 tgtatgggcc cccgtcggca gttgggtcga tacgccagtg gatgaacaag taaaatttgg
     3121 cgatgagatg gtcgatcgag aatattttgc cacgtggcaa caagtgcgag aagttgcgcg
     3181 ttcccggctc gttgaggtcg cttctcatac atggaattct cactacggta ttcaggctaa
     3241 ttccaccggc agcttaatgc ctgtatatgt aaatcgtgca tattttactg accacgcacg
     3301 gtatgaaacc gcagcagaat accgggaaag aattcgtctg gatgctgtaa aaatgacgga
     3361 atacctgcgt acaaaggctg aggtaaatcc acacgttttt gtttggcctt atggcgaagc
     3421 gaatggcata gcgatagagg aattaaaaaa actcggttat gacatgtttt tcacccttga
     3481 atcaggtttg gcaaatgcgt cacaattgga ttccattccg cgagtattaa tcgccaataa
     3541 tccctcatta aaagagtttg cccagaaaat tattaccgta caggaaaaat caccacaacg
     3601 gataatgcat atcgatcttg attacgttta tgacgaaaac cgccagcaaa tggatcgcaa
     3661 tattgatgtg ctaattcagc gggtgaaaga tatgcaaata tcaaccgtgt atttgcaggc
     3721 atttgctgat cccgatggtg atgggctggt caaagaggtc tggtttccaa atcatttgct
     3781 accaatgaaa gcagatattt ttagtcgggt tgcctggcaa ttacgtaccc gctcaggtgt
     3841 aaacatctat gcgtggatgc cggtattaag ctgggattta gatcccacat taacgcgagt
     3901 aaaatactta ccaacagggg agaaaaaagc acaaattcat cctgaacaat atcaccgtct
     3961 ctctcctttc gatgacagag tcagagcaca agttggcatg ttatatgaag atcttgccgg
     4021 acatgctgct tttgatggca tattgttcca cgatgatgct ttgctttcag attatgaaga
     4081 tgccagtgca ccggctatca cggcttatca gcaagcaggc tttagcggga gtctgagcga
     4141 aattcgataa aacccggagc aatttaaaca gtgggcccgc tttaaaagtc gtgcgttaac
     4201 tgacttcact ttagaactta gtgcgcgcgt aaaagccatt cgcggtccac atattaaaac
     4261 tgcacgaaat atttttgcac ttccggtaat acaacctgaa agtgaagcct ggtttgcaca
     4321 gaattatgct gatttcctaa aaagctatga ctggaccgct attatggcta tgccttatat
     4381 ggaaggtgtc gcagaaaaat cggctgacca atggttaata caattgacca atcaaattaa
     4441 aaacatccct caggctaaag acaaatctat tttagaatta caggcacaaa actggcagaa
     4501 aaatggtcag catcaggcta ttccttcgca acaactcgct cactggatga gcctattaca
     4561 actgaatgga gtgaaaaact atggttatta tcccgacaat tttctgcata a
//
LOCUS       DAJTQY010000191         4560 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.193, whole genome shotgun sequence.
ACCESSION   DAJTQY010000191 DAJTQY010000000
VERSION     DAJTQY010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4560
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.193"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            294..854
                     /locus_tag="OO349_003926"
     CDS             294..854
                     /locus_tag="OO349_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE4 domain-containing protein"
                     /protein_id="HCR5868820.1"
                     /translation="MTTVQFLYLLIIERDLQPTDWCSFMEKDYLRISSTVLVSLLFGL
                     ALVLVNSWFNQPGVEEVVPRSTYLMVMIALFFIDTVAFIFMQLYFIYDRRQFSNCILS
                     LAFLSCLIYFVKTVIIIQQIIEGRLTSSVVQNDIAIYYLFRQMSLCILIFLALMNKVS
                     ENTKQRNLFSKKMTLCISLFLFLGGR"
     gene            797..1186
                     /locus_tag="OO349_003927"
                     /pseudo
     CDS             797..1186
                     /locus_tag="OO349_003927"
                     /inference="COORDINATES: protein motif:HMM:NF028467.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MASE4 domain-containing protein"
     gene            1166..1723
                     /locus_tag="OO349_003928"
                     /pseudo
     CDS             1166..1723
                     /locus_tag="OO349_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001420326.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
     gene            1821..3430
                     /locus_tag="OO349_003929"
                     /pseudo
     CDS             1821..3430
                     /locus_tag="OO349_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309299.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(3531..4022)
                     /locus_tag="OO349_003930"
     CDS             complement(3531..4022)
                     /locus_tag="OO349_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FidL"
                     /protein_id="HCR5868821.1"
                     /translation="MTQRYFLFSGIILCAFIAAILSHIAFHHSNEPAEQNISCNAHVI
                     NFTDHTKMDQYFSLNMISENNTGNMYLTGAYTVDGKKMGFIRRYVSFTYKKFRDTVYL
                     TTVKIQKIQKDDNISDEILENITSDFFIKADTSINYYVTRQNDYNYIFSTGRTPRFIC
                     ITH"
     gene            complement(4027..4203)
                     /locus_tag="OO349_003931"
     CDS             complement(4027..4203)
                     /locus_tag="OO349_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001411776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR5868822.1"
                     /translation="MLSPIIEKLQKMEVNCKIHPYIYIATNDSMSGFYAFTCSQMLNS
                     ESPNNCNSLYYKAE"
BASE COUNT         1401 a          728 c          901 g         1530 t
ORIGIN      
        1 aatcccaatg cctttttcaa aataaattag gattaaaata attaaatcag taaattccga
       61 tgcatgattt cggatttttc gaaagtcctg atgaaaggct gcgctacggc actcacggat
      121 ttaattgtta ttcctaatgt atctatgaat taatgtttta taaggatttt ttataaccat
      181 ttataagtat tttcaagcct ggcttgttgc aaacaatgta taaagcactt aggcaataat
      241 aattacattc agcaactatc atcatcggta ttgtttgtgg gcggaatttc atattgacaa
      301 cagtacagtt cttatatcta ttaataatag aaagagatct acaacctaca gattggtgta
      361 gctttatgga aaaagactat ttgagaatta gtagtactgt attagtgagc ttattgtttg
      421 ggcttgcttt ggttttggtg aatagttggt ttaatcagcc aggcgttgag gaagttgttc
      481 cacgatcaac ctatctgatg gtaatgattg ctttattttt tatcgatact gttgcattta
      541 tttttatgca gttgtatttc atttatgacc gtaggcaatt ttcaaactgt atacttagtt
      601 tggcttttct gagttgtttg atttactttg ttaaaaccgt cattatcatt cagcaaatta
      661 ttgaggggcg tttgacaagc agtgttgtcc aaaatgacat tgcaatctat tatttgtttc
      721 gtcagatgag tttgtgcata ttaatatttc tggcattgat gaataaagtt agtgaaaaca
      781 caaaacagcg caatttattt tccaaaaaaa tgactttgtg cataagtttg tttttgtttt
      841 tggggggccg atagttgctc acatactttc tagtcattat gagagctaca atttacacat
      901 tgccgagtta accaatgaaa atggtcaggt ggtttggaaa gcctcatatg ttaccataat
      961 gattttcatg tggttaacct tgctttcggt taatctatac tttaatggtt tacgctatga
     1021 tatctggaat ggagtgacgg tgattgcttt ttgtgccgta ctgtacaata tctctctgtt
     1081 atttatgagc aggtatagcg tgtccacttg gtatattagt cgcactattg aagtagtcag
     1141 taagttaacc gttatggtaa tatttatgtg ccatatattc agtgcgctac gagtaacaaa
     1201 gaacattgca catcgcgatc ccttaaccaa tatatttaac agaaattatt tttttaatga
     1261 actgacagtt caatcagcat cagccaaaaa aacgccttat tgtgtcatga ttatggatat
     1321 cgaccacttc aaagaagtca acgacacctg agggcatccg gttggcgatc aggtgataaa
     1381 aacagtggtg aatatcattg ggaaaagcat acgaccagat gaccttttag cgcgcgtcgg
     1441 cggcgaagag tttggcgtct tgctgacgga catcgatact gaacgtgcga aagctttagc
     1501 ggaaaggatt cgggaaaatg ttgagcgttt aactggcgat aatcctgaat acgctatacc
     1561 acaaaaagtg acgattagta ttggcgctgt tgtgactcag gaaaatgcgt taaacccgaa
     1621 tgagatctat cgactggctg ataacgcact atacgaggcg aaagagaccg ggcgtaataa
     1681 ggtggttgtg agggatgtgg tgagtttttg tgagtcacca taaagcggca ttttagcggg
     1741 tgattttggt aagcgctaaa tagctattcg cagacttgcg taacaatttt cagaaatatc
     1801 atagtactat atgatcttaa atgcttatca ttgatcaatc tgctatcacc cgcttttact
     1861 ggcaattgta ctataatttc ttaggttgca tcaccctgat gcgtggggaa acatgtatgg
     1921 tattcaacaa aaaaatgttt gtactcatta taataccagg cattctgggg gtgttactat
     1981 cgtttgctat gtcaatattt caaatgaata gagatacgac aattaccgcc ggtattctgt
     2041 taaaacagct tgataatgtt acgcaaattg tccagcatac gacaaagctt acctctattc
     2101 tggtgatgaa gccctgcaaa gacattcttg aacagttgat tgctaacgga gcattgactc
     2161 cctatgttcg aacaacgggt cttatagaaa ataatttcca aatatgttct tcagttagcg
     2221 gtttcaaaaa aatgaatgtt aacgatgttt acggtacgag ctttcacaat aaaaataaag
     2281 aatcacgtat agtatcaatt agcggtacaa gttttgtgcc aggtaaaaca gcaatagtgt
     2341 tccttatgcc aatagggaat gatatgacag cttttagtat tgtagagtca cgatatatct
     2401 atgatctaat ggatgttctt gatgatgaga atgatgattc attttctctt cgttttactg
     2461 aagggcctgc aattattagc ggtgtaaata ataatgacag gttatatatg ttaaaaagag
     2521 attttaattc tgcgatcagt caggctcgtc ttacagttac tacgccgatg atatcgttat
     2581 atccctatat cattagcaat gtgctttata ttttgccgtt atctattatt ctctctttta
     2641 ttctctattt cttatggcag cactggatgt cacggaaaat gtcgctggca gaagaaatta
     2701 aaaaaggaat gtcatccgga gaattttctg tgcattatca accagtgtgc gacacgacaa
     2761 ccaaagcgtg tctgggggtt gaagcgttaa tgcgctggca acgcgaagat ggtaaaaata
     2821 tatctccggt agtttttatc cgcgctgcag aagaggaaaa tttaataata ccgttaacta
     2881 aacatctctt tgaactgatt attcaggatg tacaaagctg gaaagtgaaa aagccgtttc
     2941 accttgggat aaatattgcc gcggaacatc ttgcacatcc tgactttgtt ggtgatgttt
     3001 tacatattaa aaatgcaata tctgataaat ttaatattgt ccttgagatt accgaacgca
     3061 atctggttga agacactgat cttgcattac agaaaataaa tgaattaagg atccatggct
     3121 gtgaatttgc agtcgatgat tttggcacgg gatattgttc ccttggcctt ctgcaaaagt
     3181 tgtctgttga ttatcttaaa atagataaaa gctttataga cacacttact acagcggaag
     3241 acgaaacgcc agttttagat attattatta agctgagtaa tcggctcaat ttgataacga
     3301 ttgcagaagg tgtcagtact cctcaccagc agaatatctt attcataata acgtgacatt
     3361 agttcaggga tacttatatg aaaaaccaat gaaagcgaca gagttttatc agtggtatgt
     3421 aaacagataa ataattaaaa atattggaat ggttttgtat tgtcttaaaa tataaacaaa
     3481 gcaatcagaa tgttattaac tgatgacaaa cgccaagatc aacaatgctg ttagtgagta
     3541 atacatatga atcgtggtgt cctgcctgta gagaatatgt agttataatc attttgacgc
     3601 gtaacataat aattaattga agtgtctgct tttatgaaga aatcagaagt gatattttca
     3661 aggatttcat cgctgatgtt atcatctttt tgtatttttt ggattttaac tgtggtgaga
     3721 taaacggtat cacggaattt cttgtatgta aaactcacat atcggcggat gaagcccatt
     3781 ttttttccat ctaccgtgta tgcgcccgtg agatacatgt ttcctgtgtt attttcagaa
     3841 atcatattta acgaaaaata ttgatccatt tttgtatgat cggtgaaatt aataacatgg
     3901 gcattacatg atatgttttg ctctgctggt tcattggaat gatgaaacgc aatatggctc
     3961 agaattgctg caataaaagc acataaaatt attcccgaaa acagaaaata tctttgagtc
     4021 atatgattac tccgctttat aatagagaga gttgcaatta tttggtgatt cagagtttaa
     4081 catttgacta caggtgaaag cataaaaccc gctcatgcta tcatttgttg caatgtaaat
     4141 gtaaggatgt attttgcagt tgacttccat tttctgcaat ttttcaataa ttggtgatag
     4201 catgtctacg gaattaaagt aattattatc atagaaataa ctgcatcctt cctgagtagg
     4261 gtgttgagca taatttgaga aagcatctat tttttttata gtgtgagatt tgctttgaaa
     4321 agccatatcg aaaaaataag aagacatata aactatcccc catgccgcaa aaaaaaacta
     4381 taagtaatgt attctttgtg gtaaatatac cttttgcaac gtaattttta tttatatttt
     4441 cagtgtgggt gccattctct tcactctcaa cactttcatt ctcattgtga ttattgtcga
     4501 gaacagttat gttgatttct tcattgattt tgaagccttt ccgtggcacc gttctgataa
//
LOCUS       DAJTQY010000192         4551 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.194, whole genome shotgun sequence.
ACCESSION   DAJTQY010000192 DAJTQY010000000
VERSION     DAJTQY010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4551
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.194"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            <1..1224
                     /locus_tag="OO349_003932"
     CDS             <1..1224
                     /locus_tag="OO349_003932"
                     /inference="COORDINATES: protein motif:HMM:NF027197.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HCR5868823.1"
                     /translation="FPYGIFQQRNVLASWLATGCGVALYLALTARTRSLTLICLLSLY
                     PLSTALVLTQSRVGLLGLFCVIVLAALADRPRWRGRPLSGLLRLIFVASLLCWCVGIS
                     NYAMPGETTADFTHSSSNDQRIRILKGAISLIQQRPLTGWGLGSFEATFPQALEDVGV
                     TNIESDTATHPHNELLFVTAEGGIVALFGFLLLSGVWLWPCICRLKMTNNNAHHQCEK
                     WFIALMGLPLVIHMMTEYPLYTSVPHLMLLLLLFRIGMPENVMNQVRIPKTVRLGILP
                     VIVLIMTAALVILVAGFDAQRELTRAEADMNEGFIPRLPEHSWKYLTQAERLERDQHL
                     LIANKPGFVRDTESMSLFTVWGNKWLAIHNDADVSAAMIFIAHRRGDYLTAEKIRKRA
                     ARVFVNDERFTRGGN"
     gene            1224..1898
                     /locus_tag="OO349_003933"
     CDS             1224..1898
                     /locus_tag="OO349_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5868824.1"
                     /translation="MVNDLTYKKLESTNINCNILSPENTTWTNVTVIGLDLFAICGVC
                     SLLKRVGGVKLQVFTSLPTDMNVLTDSSIVIWVKMRHDGLPELAGHIVKVCRRYRLMR
                     QLVISDAIPEGMTNDIGPLSRVWLTHGSAKVDILLSSLRKVLNAHCLPGPLFIKRLGR
                     VQWRVLLLRAKGISTRKIAEICGIGLKTVSAHESAIRERLGTRNKSEYVWLVRNAQLI
                     QQAIPA"
     gene            2426..3187
                     /locus_tag="OO349_003934"
     CDS             2426..3187
                     /locus_tag="OO349_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCR5868825.1"
                     /translation="MKNFNVLTKVVFCLSFLSSAFPTYAADDVKVTQNTSVFKLKLSA
                     TRLIYEQGSDGAAITVTNPQDWPMLVQSKVFEEDKVTAAPFIVTPPLFRLNPGQQNKM
                     WVVQTAPALQDREKLHWLCATGITPKKSDVWAEKDKEKIAENVNLNIQVNVHTCIKLI
                     TRPSGIEGKMVNASEKLSWKKQGDKIVAVNQSPFYINLSSLQVGGSKIEKLDYIAPFS
                     QHEFVMPKTSGQDIKWSVITDEGGESRLFISHLQS"
     gene            3231..4310
                     /locus_tag="OO349_003935"
     CDS             3231..4310
                     /locus_tag="OO349_003935"
                     /inference="COORDINATES: protein motif:HMM:NF025320.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005093492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5868826.1"
                     /translation="MFYRLSLILIMALLAGQLSAQEWSFDSSQLEGNVSADTVAMFNQ
                     GEQLPGNYRVEIYLNGEKVDVGEFPFHRPESPEEKELVPCLTVDDLIHYGIKIDKSSS
                     DTDNKKNQCFKWNSIEGLKVNYDFDSQRVQITVPQLYLQDKKSSLAPVSLWNEGVAAF
                     RMVYQTNIDISKQNDNQSTTRNSRYGRFTPGFNLGAWRFRSSVTWSKELGQSERWQRG
                     YMWFERGINAIKSRLTLGESYTSSEVFDSIPFRGGMLATDDAMTPPEDSYYTPVVHGI
                     AQSEAQVIIKQNGQIIFTRSVPPGPFALDNLPTLAVGGELDVTVRESNGEEQYFSVPF
                     QTPAIALHEGYFKYSVMGGNIKKKV"
     gene            4307..>4551
                     /locus_tag="OO349_003936"
     CDS             4307..>4551
                     /locus_tag="OO349_003936"
                     /inference="COORDINATES: protein motif:HMM:NF012786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCR5868827.1"
                     /translation="MKSSPVSVGEITAMYGLPYGVTVYGGIQNATHFNAISTGIGISL
                     GLLGSLSTDITRSIANLYYGNKYRIRYSKSISDFGTQ"
BASE COUNT         1249 a          864 c         1119 g         1319 t
ORIGIN      
        1 tttccgtatg gcattttcca gcagcgaaat gttcttgcga gctggctggc aacaggttgc
       61 ggcgtagcgt tgtatctggc tttaacggcg cgtactcgtt ctctgacgtt gatatgtttg
      121 ttgtcgttat accctctgtc tacagcttta gtccttacac aaagccgtgt cggccttctt
      181 ggtttatttt gcgtcatcgt gctggccgct ctcgcggaca gaccccgctg gcgcggacgt
      241 ccccttagcg gtctgttgcg cttgatcttc gtcgcctcct tgctgtgctg gtgtgtcggt
      301 atctcaaact atgccatgcc aggggagaca acggctgact ttacgcattc atcatcgaat
      361 gaccagcgta tacgtattct gaaaggtgcc ataagtctta ttcaacagcg ccccttaacc
      421 ggttgggggt taggcagctt tgaagcgacc ttcccgcaag ccttagagga tgtgggcgtg
      481 acaaacatag agagtgatac ggctacgcat ccccataacg aactgctgtt cgtaacggct
      541 gaaggtggca tcgtcgcctt gtttggtttt ttgctgctat caggcgtttg gctgtggccg
      601 tgtatatgcc gcctgaaaat gacaaacaat aacgcgcacc atcaatgtga aaagtggttt
      661 attgccttaa tggggttgcc tttagtcatt catatgatga ccgaatatcc tctctatacg
      721 tctgttcccc atttgatgct gttgctgctc cttttccgca tcggaatgcc ggaaaacgtg
      781 atgaatcagg tacgtatacc taaaacagtc cggttgggta ttctgcctgt cattgtgctc
      841 attatgaccg ccgcgctggt catcctggta gcgggatttg atgcacagcg ggaactgacc
      901 agagctgaag ctgatatgaa cgaaggattt atccctcgcc ttccggaaca tagctggaaa
      961 tacctgacgc aggcagaacg tcttgagcgc gatcagcatc tgcttattgc gaataaaccg
     1021 gggttcgttc gcgatacgga aagcatgtct cttttcactg tgtggggaaa taaatggttg
     1081 gcaatccaca atgatgcaga cgtaagcgct gcaatgatat tcattgcgca caggcgcggt
     1141 gattatctga cagctgaaaa aataagaaaa cgcgcagctc gggtatttgt aaatgacgaa
     1201 cgttttactc gcgggggaaa ctaatggtta acgatctgac gtataagaaa ttagaaagca
     1261 caaacatcaa ctgtaatatt ctctcgccag aaaataccac atggacaaat gtcacggtga
     1321 ttggcctgga tctttttgcc atttgcggcg tgtgcagttt gctcaagcgt gttgggggag
     1381 tgaaacttca ggttttcact tccttaccca ctgatatgaa tgttctgact gacagtagca
     1441 tcgtgatatg ggtaaaaatg cgtcacgatg gtttgcctga attagccgga catattgtga
     1501 aggtttgtcg tcgataccgt ctgatgcggc aactcgttat tagcgatgct attcctgaag
     1561 gaatgacgaa tgatatcggg cctctttccc gagtatggct tactcatggg agtgcaaaag
     1621 tggatatttt attatcttca ttacggaagg tgttaaatgc tcattgtttg cctggtcctt
     1681 tgtttataaa acgcctggga agagtgcaat ggagggttct tttactccgc gcgaagggga
     1741 ttagcacacg taaaattgca gaaatctgtg gtattggcct caagaccgtt agcgcccatg
     1801 agtccgcaat tcgagaacga ttaggcacca ggaataaaag tgagtacgtc tggttagtac
     1861 gtaatgctca acttatacaa caggcgatac ccgcgtaggg aagagatgcg tcaaggtatg
     1921 aaagatgtaa caatatgcga acaaagcaaa aagtataatt gatgcgtcat agataactct
     1981 ctttttcgat gacgaaatcg ctgagcatgc gtggtgaggt tagtaataga tgggtgctat
     2041 gttttgccac gggtttgtag ctgtgaaacc tggaaaaaaa taagaattaa aaatatttaa
     2101 agagagttaa taaggttctg gttgtaattg tcttggtcaa aaaaaagata ttgtcaatgt
     2161 tgttttttaa attttcaggt tttggttaaa tatttgctta caaatatagg tgattttttt
     2221 attgtaggct tttgaaggct attataactg tgcgctaaat taatgcgttg aataaaagaa
     2281 ataacaaaat taagaaaagc catatttacg atatgggcaa cataaatata atctaaaaaa
     2341 gagtaaggcg ttgtaggttg gttccgcgtt cattaccttg ttagtgaacg cggatttatc
     2401 agttgtcgaa atggttttta taataatgaa aaattttaat gtactaacca aagtcgtatt
     2461 ttgcttgtcg tttttatcaa gcgcatttcc cacctatgct gcggatgatg tcaaagtgac
     2521 tcaaaacact tcagttttta aactgaagct tagtgcaacg cgattaatct atgaacaagg
     2581 gtcggatggc gcagcgataa ctgtgacaaa tccgcaggat tggccgatgc ttgtgcagag
     2641 taaagtcttt gaagaagata aagtaactgc agcgcccttt attgtcactc cgccactgtt
     2701 caggcttaat ccaggccagc aaaataaaat gtgggttgtg cagactgcgc cagctttaca
     2761 agacagggaa aaattacact ggctatgcgc caccggcatt acgcctaaaa agagtgatgt
     2821 ttgggccgag aaagataaag aaaaaattgc tgaaaatgtg aacctgaaca tccaggttaa
     2881 cgtacatacc tgcataaaac ttattaccag gccatctggt attgaaggca agatggttaa
     2941 tgcgagtgag aagttatcgt ggaaaaagca gggtgataaa attgtcgcgg tcaatcagtc
     3001 gccgttctac atcaaccttt cttctttaca ggttggcggt agcaagattg agaaactaga
     3061 ttatattgcg ccgttttcgc aacatgagtt tgttatgcca aaaactagcg gccaggatat
     3121 taaatggtct gtgattactg atgaaggtgg tgagagtcgt ttgtttatat ctcatttgca
     3181 atcataatgt attacatttt tattgaatat ttttatgcca tgcatattcc atgttttatc
     3241 gactgtcatt aattttgata atggccttac ttgcgggtca gttaagtgcc caggagtggt
     3301 catttgattc ttcacagttg gaaggaaacg ttagtgcgga cactgtggca atgtttaatc
     3361 agggagagca actccccgga aattatcgtg tggaaattta tcttaacggt gaaaaagttg
     3421 atgttgggga gtttcccttt catcgtccgg aatcccctga agaaaaagag cttgttccat
     3481 gtttaaccgt tgatgacctt atccactatg gcattaaaat tgataaaagc agcagtgata
     3541 ctgacaataa aaagaatcag tgttttaaat ggaactctat tgaaggtttg aaagtaaatt
     3601 atgattttga tagccagcgc gtacagataa ccgttccaca actgtatctg caagataaaa
     3661 aaagtagttt agcaccagtg tcgttatgga atgagggtgt agctgcattc cgcatggtgt
     3721 atcagacaaa tattgatatt tccaaacaaa atgataatca atctaccacg cgtaattctc
     3781 gttatggcag gttcactcca gggtttaatc ttggtgcctg gaggtttcgt agttcagtga
     3841 catggagcaa ggaacttggc cagtcagaaa gatggcaaag gggttacatg tggtttgagc
     3901 gaggcataaa tgcgattaaa agccgattaa cgcttggaga atcttatacg tcgtctgagg
     3961 tgtttgacag catacctttt cgcggcggga tgttagcgac cgacgatgcg atgactccgc
     4021 cggaggacag ctactacaca cctgtggtgc atggaatagc acaaagtgag gcgcaggtta
     4081 ttattaaaca aaatgggcaa attattttta cccgttctgt gccgccaggg ccttttgcgc
     4141 tggataattt accaacgctg gccgttggcg gggagcttga tgttacagtg agagagagta
     4201 atggcgagga gcagtatttt tccgtgccat ttcaaacgcc ggccatcgct ctgcatgaag
     4261 gatatttcaa atatagtgtg atggggggga atataaagaa gaaggtataa aaagctcccc
     4321 tgtctccgtg ggcgaaatca cggcgatgta cgggcttcct tatggcgtca cggtgtatgg
     4381 agggattcaa aacgccactc attttaatgc aatatctacg ggtattggca tttctctggg
     4441 gctgttaggg agtttatcga cagatattac gcgttcgatt gcgaatttat attatgggaa
     4501 taaatatcgt attcgttata gtaaatccat tagtgatttt ggtactcaat t
//
LOCUS       DAJTQY010000193         4462 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.195, whole genome shotgun sequence.
ACCESSION   DAJTQY010000193 DAJTQY010000000
VERSION     DAJTQY010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4462
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.195"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            <1..527
                     /locus_tag="OO349_003937"
     CDS             <1..527
                     /locus_tag="OO349_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HCR5868828.1"
                     /translation="TTGRAEATILVDIAHQFWHIPHEKIWIEDQSTNCGENARFSITL
                     LNQAVERVHTAIVVQDPTMQRRTMATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVI
                     FINQLQGLWPVERYLSLLTGELPRLHDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKH
                     DAVLIEAMESRSLR"
     gene            724..2163
                     /gene="aldA"
                     /locus_tag="OO349_003938"
     CDS             724..2163
                     /gene="aldA"
                     /locus_tag="OO349_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HCR5868829.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLCKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNLLGEALQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(2205..3206)
                     /gene="gap"
                     /locus_tag="OO349_003939"
     CDS             complement(2205..3206)
                     /gene="gap"
                     /locus_tag="OO349_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCR5868830.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     ADKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            3395..3925
                     /gene="cybB"
                     /locus_tag="OO349_003940"
     CDS             3395..3925
                     /gene="cybB"
                     /locus_tag="OO349_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HCR5868831.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            4170..4343
                     /locus_tag="OO349_003941"
     CDS             4170..4343
                     /locus_tag="OO349_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5868832.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
BASE COUNT         1142 a         1030 c         1118 g         1172 t
ORIGIN      
        1 gcaccactgg cagagcagaa gcgaccatcc tggtggatat cgctcatcag ttctggcata
       61 ttccgcatga aaaaatctgg attgaagacc agtcaacaaa ctgcggtgaa aacgcacgct
      121 ttagcatcac gctattgaat caggccgtag aacgagttca tacggctatc gttgtacagg
      181 accccaccat gcagcggcgc acgatggcaa cgttccgccg tatgaccggg gacaatcccg
      241 atgcaccacg ctggttaagt tatcccggat tcgttcctca gttaggaaat aacgcagaca
      301 gtgtaatctt tattaatcag ttacaaggat tatggccggt tgagcgttat ctctcactac
      361 tcactggcga gctgccgcgt ttacacgatg atagcgatgg ctacggtccc cgcgggcgag
      421 attttatcgt tcacgttgat tttccggcag aagtcatcca tgcatggcaa acgctgaaac
      481 atgatgcggt gctcatcgag gcgatggaaa gtcgctcgtt acgttaaaaa ttgcccgttt
      541 gtgaaccact tgtttgcaaa cgggcatgac tcctgacttt tatttctgcc ttttattcct
      601 tttacacttg tttttatgaa gccctccaca gacttgtcct ttcccgatgc cgtctctctg
      661 atgattgatg ttaattaaca atatattcac cgaaaacaaa catataaatc acaagagtcg
      721 cccatgtcag tacccgttca acatcctatg tatatcgatg gacagtttgt tacctggcgt
      781 ggagacgcat ggattgatgt ggtaaaccct gctacagagg ctgtcatttc ccgcataccc
      841 gatggtcagg ccgaggatgc ccgtaaggca atcgatgcag cagaacgtgc acaaccagaa
      901 tgggaagcgt tgcctgctat tgaacgcgcc agttggttgt gcaaaatctc cgccgggatc
      961 cgcgaacgcg ccagtgaaat cagtgcgctg attgttgaag aagggggcaa gatccagcag
     1021 ctagctgaag tcgaagtggc ttttactgcc gactatatcg attacatggc ggagtgggca
     1081 cggcgttacg agggcgagat tattcaaagc gatcgtccgg gagaaaatat tcttctgttt
     1141 aaacgtgcgc ttggtgtgac taccggcatt ctgccgtgga actttccgtt cttcctcatt
     1201 gcccgcaaaa tggctcccgc tcttttgacc ggtaatacca tcgtcattaa acccagtgaa
     1261 tttacgccaa acaatgcgat tgcattcgcc aaaatcgtcg atgaaatagg ccttccgcgc
     1321 ggcgtgttta accttgtact ggggcgtggt gaaaccgttg ggcaagaact ggcgggtaac
     1381 ccaaaggtcg caatggtcag tatgacaggc agcgtctctg caggtgagaa gatcatggcg
     1441 actgcggcga aaaacatcac caaagtgtgt ctggaattgg gtggtaaagc accggctatc
     1501 gtaatggacg atgccgatct tgaactggca gtcaaagcca tcgttgattc acgcgtcatt
     1561 aatagtgggc aagtgtgtaa ctgtgcagaa cgtgtttatg tacagaaagg catttatgat
     1621 cagttcgtca atctgctggg tgaagcgttg caggcggttc aatttggtaa ccccgctgaa
     1681 cgcaacgaca ttgcgatggg gccgttgatt aacgccgcgg cgctggaaag ggttgagcaa
     1741 aaagtggcgc gcgcagtaga agaaggggcg agagtggcgt tgggtggcaa agcggtagag
     1801 gggaaaggat attattatcc gccgacattg ctgctggatg ttcgccagga aatgtcgatt
     1861 atgcatgagg aaacctttgg cccggtgctg ccagttgtcg catttgacac gctggaagat
     1921 gctatctcaa tggctaatga cagtgattac ggcctgacct catcaatcta tacccaaaat
     1981 ctgaacgtcg cgatgaaagc cattaaaggg ctgaagtttg gtgaaactta catcaaccgt
     2041 gaaaacttcg aagctatgca aggtttccac gccggatggc gtaaatccgg tattggcggc
     2101 gcagatggta aacatggcct gcatgaatat ctgcagaccc aggtggttta tttacagtct
     2161 taatgagtga aagaggcgga ggttttttcc tccgcctgtg cgcgtcagag tttagcgaat
     2221 ttttcgaggg tgcgaataag ctgcgtgacg aagccatatt cgttatcgta ccaggcgacc
     2281 gttttcacca gttgtaaatc gcccacggcg gtaatttccg tttgcgtggc atcaaacact
     2341 gaaccgaaat ggctgccaat gatatcggaa gagactattt cttcatcggt ataaccaaat
     2401 gactcgttat tggtggttgc ttgtttaagt gcgttattca cctcttcggc agtcactttt
     2461 tttccgagaa tggacaccag ctcagtgacc gaacctgttt tcaccggcac gcgttgcgca
     2521 tgacctttca gtttgccgct cagttccggg atcaccagac caatggcttt tgccgccccc
     2581 gtagtgtggg gaatgatatt ttctgccgct gcgcgtgaag cacgtaaatc tttaccacgc
     2641 gggccatcca ccagtgactg ggtgccagta taggcatgaa tggtcgtcat cgtgccgact
     2701 tctatcccga aactgtcatg caaggctttg gccatcggcg caaggcagtt agtggtgcat
     2761 gacgccacgg aaacaatggt gtcgttgcca tccagagtgt cgtcattaac gttataaacg
     2821 atggttttca tttcaccggc aggagcggaa atcaacacct tcttcgcacc agcatcaaga
     2881 tgcgcctgcg atttatcggc ggaggtataa aagccagtac attcgacaat gatttctgcc
     2941 cctttcgctt tccacggaat atttttagcc tctttttcgg cgtaaaccgc gatacttttc
     3001 ccatcaacga taagtgaatc ttccgtaaaa tcaacgctcc aggggaacgg accatagttt
     3061 gaatcatgtt tcagcagata ggcgagaatt tttggggaag taagatcatt aatagcgaca
     3121 acgtctatgt tgcttttgac ttcaagtaaa cgacgcaaca ccagtcgacc gatacgacca
     3181 aaaccgttaa taccaacttt actcatggtt ttctcctgtc aggaacgttc ggatgaaaat
     3241 tgatcctttc caggcttaga ccaggattgt gggatgggca atctccattc ttacagtgaa
     3301 acgtaacgta ctgaaaacgg gtgaacaata tttaatgaaa ttttgagaaa agcccgttat
     3361 gttaacggaa aattatgtta aagcaggaaa tgttatggaa aataaatact caaggttaca
     3421 aatcagcatt cactggctgg tctttttgct ggttatcgca gcgtattgcg caatggagtt
     3481 tcgtggtttc ttcccacgta gcgatcggcc gctcatcaac atgattcatg tttcctgtgg
     3541 catctcaatt ctcgtgctga tggtggttcg tcttttgtta agactgaaat acccaacccc
     3601 gccgattata cctaagccga aaccgatgat gacgggactg gcgcatttgg gacatttggt
     3661 gatttatctc ctgtttattg cgctgccagt gattggtttg gtgatgatgt ataaccgggg
     3721 caacccgtgg tttgcgtttg gtttgacgat gccttacgct tcagaggcca atttcgaacg
     3781 ggttgatagc ttaaagtcgt ggcatgagac gctggcgaat ctgggatatt ttgtcatcgg
     3841 gttgcacgct gcggctgcac tggcacacca ctatttctgg aaggacaaca cacttctacg
     3901 catgatgccg cgtaaacgtt cctgaaggat atttaaagaa aacgcctgta ctaaaaccga
     3961 cccgtggtac aggcgaagaa tacgggtcta catcggaagc gcctattata tttatttgta
     4021 tgacaaataa aaccataatc cttgcccata cgtccatctg gcttattttt aatcaattca
     4081 cccgatcttt gatctcatca acggtatcaa aataaaccag cgtataacgt tcaaatcgca
     4141 cataagattc gataaaccat ggaggttata tgaaaaaact ggcacttatt ttgtttatgg
     4201 gaacgcttgt ttccttttat gccgatgccg ggcgcaaacc ctgttctggt tcgaaagggg
     4261 ggatctcaca ctgtacggca ggcggcaaat ttgtctgtaa tgatggttct attagtgcat
     4321 cgaaaaaaac atgcactaac tgaagtgtaa aaggggtgcc atgagaaaat gaatctgcta
     4381 gtcagatgcg cggggaaaat ccccgcgctt gcccttacct ggacgtgcag gccatgagcg
     4441 cagcaacctc cttatcaccg tc
//
LOCUS       DAJTQY010000194         4328 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.196, whole genome shotgun sequence.
ACCESSION   DAJTQY010000194 DAJTQY010000000
VERSION     DAJTQY010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4328
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.196"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(<1..904)
                     /locus_tag="OO349_003942"
     CDS             complement(<1..904)
                     /locus_tag="OO349_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HCR5868833.1"
                     /translation="MNRRRKLLIPLLFCGAMLTACDDKSAENAAAMTPEVGVVTLSPG
                     SVNVLSELPGRTVPYEVAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELN
                     SAKGSLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAV
                     EQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQD
                     FLRMKEEVASGQIKQVQGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIF
                     PNPNGDLLPGMYVTALVDEGSRQNV"
     gene            964..1242
                     /locus_tag="OO349_003943"
     CDS             964..1242
                     /locus_tag="OO349_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5868834.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNQVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(1243..1770)
                     /gene="gadE"
                     /locus_tag="OO349_003944"
     CDS             complement(1243..1770)
                     /gene="gadE"
                     /locus_tag="OO349_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HCR5868835.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(2569..3141)
                     /gene="hdeD"
                     /locus_tag="OO349_003945"
     CDS             complement(2569..3141)
                     /gene="hdeD"
                     /locus_tag="OO349_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HCR5868836.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            3396..3728
                     /gene="hdeA"
                     /locus_tag="OO349_003946"
     CDS             3396..3728
                     /gene="hdeA"
                     /locus_tag="OO349_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HCR5868837.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            3844..4170
                     /gene="hdeB"
                     /locus_tag="OO349_003947"
     CDS             3844..4170
                     /gene="hdeB"
                     /locus_tag="OO349_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HCR5868838.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNENDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            4234..>4328
                     /locus_tag="OO349_003948"
     CDS             4234..>4328
                     /locus_tag="OO349_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="inner membrane protein involved in cell
                     density-dependent acid resistance; part of the acid
                     fitness island (AFI) of E. coli; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HCR5868839.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAG"
BASE COUNT         1275 a          923 c          926 g         1204 t
ORIGIN      
        1 atacattctg gcggctacct tcatccacta atgccgtgac atacatgcca ggcagcaagt
       61 caccatttgg gttggggaaa atcgcccgta acgtcacgga gcccgtggtt tcatcaaccg
      121 tcgggtcgga gaatttcagc gtgccggtct ggctgtaacg tttaccattt tccagattga
      181 gctgtactgg cgtactgccc tgaacctgtt tgatttgccc actggcgacc tcttctttca
      241 tgcgtaagaa atcttgcacc gactgcgtga gatcgacata aatcgggtcc agacgttgta
      301 cggtaaccag cgaatctgcc tgattagcgg taacgagtgc gccgacggtc accgacgatt
      361 tcccgctgac gcccgtaatc ggcgaggtga cattcgcgta ttgcagattg atcgtcgcct
      421 gttcaacagc cgctttggcg acggtgacat tggcttctgc ttcattcagc tgggtgcgcg
      481 cggtgtcgta atcctgacgg ctaacgtagt tggtcttcag caacgatgcc tggcggttaa
      541 aggtgatgcg ggcattgctg gcggtagaga gcgctttcgc cagcgagcct ttggcggagt
      601 ttagctcggc ctgtaaaggt gcaggatcaa tctgatacag cgaatcgccc tggttcactt
      661 tatcgccttc gataaagttg cgtttaatga taataccgcc cacctgggga cgtatctcgg
      721 caacttcata aggaacggtt ctaccgggca attcgctcaa cacattgacc gaaccagggg
      781 agagtgtgac gacaccgacc tcaggcgtca tggcggcggc gttttccgcc gatttgtcat
      841 cgcaggcggt gagcatcgcg ccgcagaata acaacggtat taacagcttt cttcttctgt
      901 tcattttagt ccctgaaaat tcttgagaaa taacggttct ttaaattgcg cgcgaggaga
      961 gatatgaggg atttacagac cagcggcata gtggggttgt cagccagtaa ggtgggatac
     1021 aggcacagtg atcgacatgg tgaggtcaac gacaaaaaaa atgttgccgt tctgccaaca
     1081 gttcctgcca gcattcgggc aacgaagggt tctactggtg gatacacata ccaggggaat
     1141 caagtcgttt tagcctctcc tttagtaatc accgggggta atgaaatcag catctgcatc
     1201 cctcgtcatg ccagccatca atttcagttg cttatgtcct gactaaaaat aagatgtgat
     1261 acccagggtg acgatgtcgc tcatacgtct tagtttcagc ttgtacataa tgttgaattt
     1321 ctgaatcttt aatgtttgtt gcgaaatatt gagcgtcgac gtgatattgc tttcattttt
     1381 atttttcaga atcagttgca taattttgaa ctgattatgt gaaaaacata attgtgaatt
     1441 cttatggggc aagtgtttac cataagtaat ctcaagaaga tttttgatac tttctttgcg
     1501 gctaacaaaa taaataggcg ctttagcttt tagtttactg atgtgataag gtgccaaaac
     1561 aataattctc tcggcatcta atttctccag aaatttaatc gcttcttcat caaggatatg
     1621 attgtgatag gtatcaatga taaccttgaa gggtttattg cctacttttt tgatctctga
     1681 cagggaattg atttttatca tttcgtgatt atctttcaac tgccaaaagc cctgtaaaag
     1741 aaaagaatct ttcgtcatga gaaaaatcat aacttgctcc ttagccgtta tcgtttcgaa
     1801 catgtcatcc ttttcattat ttacatcctt gtccgaatcg ttgttcaata tagtaaacgc
     1861 ctataagaac tttgcccttc gtgaactaat acgtctatta acactaatca atacttcaga
     1921 tttatccatc acgcactcca tatattgatc ctaagtaaga ttcctggttg ttatcagctt
     1981 gtaaatagta aacaaaaagt taacgtttgc ggattactgc ccaagaataa gttatcaata
     2041 accagtttgt gatctctgaa gaatattact aaagttaaaa tgtaatccga tttaaatatc
     2101 gagtctcctt gtttcgactt aagctggcaa ttggattgcc agctttcttt taacgcattg
     2161 aaattacctg agtaaataac gcattcatgt cttcaggttt ttatatagcg tctggagaca
     2221 cggatattta tgcaatgtaa tcaacgccag ttgcaaaaaa atgaacccgg attacttatt
     2281 taaccgccca tagcatcaac gcttacggaa ctctttgttc actttcccac agcactttta
     2341 aaacattaaa cctgactacg gaaaatatca gccatgataa tgtttgaaat ggctaatttg
     2401 ccatggagtg aaaaaaatta gatgaaattc agtaggttga aataatcact agcaggtaat
     2461 tatttcaatg atagtgcgca attgatctac aacactgcgt agcggagaga gtattaatcg
     2521 gatcatagtc acatcaagtg actatgatcc gggtgacaac cggggtaatt attgctgctt
     2581 aacgaacaaa ctggcgaagc tgaacaggct ggcggcgcta aatatcagtt caattcccac
     2641 cagtgtggaa accagcgtta cagacaccat cggcgttgca ccaaggaata tccaggcaat
     2701 gacgatatcc agcacaccaa taacgagctg tagccagctg cctttcattg aacgctgacg
     2761 ataccaactc atcaggcgaa taacccctgc aacacagaac aaaccggcaa taaatgccgc
     2821 aatggcaaaa atgcccagct ccggtgcacg gatgaagaaa tagccgatca ataaataggc
     2881 gactgcgacg aggaaaccgg ataataccgg ccagaaatta tgactgcggt tgctgaataa
     2941 cccgacaata agcgcaatac ccgagcagat taataatgca cccactactg tgcttaaaat
     3001 atcgccagag acgaacggga aactgataca cagcaacccg acgataaaca gcagcacggc
     3061 aataaactgg attgctctgc gatgcttttt cagcatctcc agatcaaact tcaaaattgt
     3121 tgccttatct atatataaca tagaaccacc ctataaaatt aagaagaaaa tcccctgcta
     3181 tcaatctatg ccaaaaacgc gtctaagaat gcagtcgatt taataaaaat ttcctaattg
     3241 cagtatctga tgcatctgta actcattgta ttgaaataaa aatatctgat tttgatattt
     3301 tccatcaaca tgacatatac agaaaaccag gttataacct cagtgtcgaa attgattcgt
     3361 gacggctctt tcactttata gttgaggata ttacgatgaa aaaagtatta ggcgttattc
     3421 ttggtggtct gcttcttctg ccagttgtga gcaatgcagc ggatgcgcaa aaagcagctg
     3481 ataacaaaaa accggtcaac tcatggacct gtgaagattt cctggctgtg gacgaatcct
     3541 tccagccaac tgcagttggt tttgctgaag cgctgaacaa caaagataaa ccagaagatg
     3601 cggttttaga tgttcagggt attgcaaccg taaccccagc tatcgttcag gcttgtactc
     3661 aggataaaca agccaacttt aaagataaag ttaaaggcga atgggacaaa attaagaaag
     3721 atatgtaatt ccgggaatgc gttacatcgt acttccttgc atattgaaca ggccggaata
     3781 tcttctttaa aagcagctat tcctcctgtt catatataat ctctatattg aatgggttac
     3841 aaaatgaata tttcatctct ccgtaaagcg tttattttta tgggcgctgt agcggctttg
     3901 tcactggtga acgcacaatc tgcgctggca gccaatgaat ctgctaaaga tatgacctgc
     3961 caggaattta ttgatctgaa tccaaaagca atgaccccgg ttgcatggtg gatgctgcat
     4021 gaagaaacag tatataaagg tggcgatacc gttactttaa atgaaaacga tctcactcaa
     4081 attcctaaag tgatcgaata ctgtaagaaa aacccgcaga aaaatttgta taccttcaaa
     4141 aatcaagcat ctaatgactt gccgaattaa tgaggtacaa gtaaaaagga gtagcaagtt
     4201 gagccatctt gctgctcctt tttgcatttt tatatgacag cagaatttat tatacgtctt
     4261 atactcgcgg caattgcctg tggcgctatt ggcatggaaa ggcaaatgcg cggcaaagga
     4321 gcagggtt
//
LOCUS       DAJTQY010000195         4326 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.197, whole genome shotgun sequence.
ACCESSION   DAJTQY010000195 DAJTQY010000000
VERSION     DAJTQY010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4326
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.197"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(<1..160)
                     /locus_tag="OO349_003949"
     CDS             complement(<1..160)
                     /locus_tag="OO349_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HCR5868840.1"
                     /translation="MDQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYL"
     gene            complement(310..471)
                     /locus_tag="OO349_003950"
     CDS             complement(310..471)
                     /locus_tag="OO349_003950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5868841.1"
                     /translation="MILRHRTGPAFQAIKDSLAVGLNALTLTDITKNATYGVEIESLV
                     LEINAPASS"
     gene            complement(468..656)
                     /locus_tag="OO349_003951"
     CDS             complement(468..656)
                     /locus_tag="OO349_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HCR5868842.1"
                     /translation="MAFKHYDVVRAAPPSDLAEKLTHKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVVVSGATEPE"
     gene            916..1251
                     /gene="iraM"
                     /locus_tag="OO349_003952"
     CDS             916..1251
                     /gene="iraM"
                     /locus_tag="OO349_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HCR5868843.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNLIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
     gene            complement(1532..1640)
                     /locus_tag="OO349_003953"
                     /pseudo
     CDS             complement(1532..1640)
                     /locus_tag="OO349_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001416037.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
     gene            complement(2163..2239)
                     /locus_tag="OO349_003954"
     tRNA            complement(2163..2239)
                     /locus_tag="OO349_003954"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2203..2205),aa:Arg,seq:tct)
     gene            complement(2341..2416)
                     /locus_tag="OO349_003955"
     tRNA            complement(2341..2416)
                     /locus_tag="OO349_003955"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2381..2383),aa:Ile,seq:cat)
     gene            complement(2561..3382)
                     /locus_tag="OO349_003956"
     CDS             complement(2561..3382)
                     /locus_tag="OO349_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCR5868844.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESIVFTNKIQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRASWEKTVKKFYDALVTRFDIEEAWAERQLKKVTR"
     gene            complement(3397..3753)
                     /locus_tag="OO349_003957"
     CDS             complement(3397..3753)
                     /locus_tag="OO349_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR5868845.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQ
                     RLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMH"
     gene            complement(3766..>4326)
                     /locus_tag="OO349_003958"
     CDS             complement(3766..>4326)
                     /locus_tag="OO349_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCR5868846.1"
                     /translation="AELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPG
                     EQTATSILKQRTKNLAPLPHAHQQQNPQQEKTVVSIAVDPESPESFMKRPKRRRWVNE
                     KYKRWVKTQPCACCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCREHHNELHADPLA
                     FEEKHGSQVDLIFRFLDHAFATGVLG"
BASE COUNT         1188 a         1179 c          856 g         1103 t
ORIGIN      
        1 tcagatatgt cagcagccca aacagcagac tccccagcac gcctattgcc gcccactgag
       61 acggggaaac cctgtccagc aactgcagga accagtagcc cgttcccacc gctgacgtgg
      121 tgtatgacac acctgttgtg attttttcca tctggtccat accccgtctc ccgttatccg
      181 gaagctgaca acaataaaaa agccaccagt taactactga tggctctgat aactcatgca
      241 agcgtctcag acgacccact gacactaccg gtgagtttaa cgataccttc catttgactg
      301 gctcactttt tatgatgatg ccggtgcatt tatctccagc accagacttt ctatctcaac
      361 gccatacgtt gcatttttgg taatatccgt cagcgtcagt gcatttagtc ccactgccag
      421 actgtctttt atggcctgga atgccgggcc agtacgatga cgtagtatca ctccggctca
      481 gttgcaccac tgaccaccac atcaccttct gctgcaatcg cctgcatcag ggtataaggg
      541 gttatggcca ccggactacc aaacggctgc cagccctctt tcagtttatg tgtcagcttt
      601 tccgcaagat ctgacggcgg cgccgccctg acaacatcat agtgtttaaa tgccatggtt
      661 ctttccaaca tctgaaaaat aattctttaa aatacctgac atgtaataca gaaaaaacac
      721 aaaaccatac cttaaataaa aacctgatta tcaagcagat atgcatggat aaactacaag
      781 acgagatata aaccaccctg catttaaata aacaataaac aacatcagaa aaataattct
      841 gctctatggt ttaattcaaa aatatcattt atacttttca gaacatcacc agcaaggcat
      901 aaacaaggaa actaaatgaa gtggattgtg attgatacag ttatccagcc atcatgcgga
      961 atatcttttt cagtcatatg gagtaaaata aaattaataa tctggtatca atcggatgct
     1021 ttcttacctc ctgaaagtat atttacactg actcacacag gcatcatgct caataacaaa
     1081 gtgctacctg taaccattta caacgtagta ccattcaata aaacattctg gaatttaatc
     1141 aaaaacagcc aggaatgccc tacaaataca gataacgtat tgaatgaatg ctttaataac
     1201 cgttgcactc tgcaaatatg tccttatgga ctaaaacaac aaagtccata aggagtttac
     1261 tcacatctga caaaatcaat ataaacagcc cctccggaga ggggctggag agtggcgcta
     1321 tgtgccattc catggtgccg ggtgcctccc ggtgaattca gtaccagcac ctgaatccgc
     1381 gattatccca tatacctact cgctgattgc ccctccgcac agggggattc accatgccag
     1441 tttcttttaa caaactcccc gcaaaccaga caacagtcaa ccgcctgaat tgtgaagtat
     1501 ttaaaaattt ctcccgctaa ctgatacccg gctaacagtc tggcgttttc tttttcagca
     1561 acgggaaagc aacaaccacc acacccgcca ccagcacacc gtcagccggc actgacatta
     1621 tccggctgct gcaatgccat taacaaaaac agtaagcaat cactttttac cgtaacaggt
     1681 gataatccag atatgtatct accccagatg agtaatccga agttcatcca taccacaggt
     1741 cctggctatt ctgttgtact cctgaacaag agcaaataat tctgaattag caaccatgaa
     1801 ctcatcgcaa accctctgta tagcatcact attcagaata ataacgtctc ttcccgaaag
     1861 acgatcagga gtacagaaca aaactgtcaa acggctgaag gcctttgctc gtgctgcatt
     1921 gactatatca atacgctgcc taaggatgaa acaccccgac gcctcatcaa tattcactct
     1981 acccacacca tatgaatgat aaatacttaa tgctgaaaaa accattagac cgtataacaa
     2041 atactcaatc aatacttaac agaactttta tttttgacaa atatataata ttttcaacaa
     2101 tatcctgagc caggtatatt tcagtataag gctctgccgg aaggaatctg gaagaatgaa
     2161 tatggcgcgc tgtactggat tcgaaccagt gaccgattgc ttagaaggca attgctctgt
     2221 ccggctgagc taacaacgct gaataccgat aatggaccgc catcggggac ccgccccccg
     2281 caccaacaac cctgttatcg tgtcgtctgc tcttcctgat aagctaatgg cggtttgtga
     2341 tggtggccct tgctggattt gaaccagcga cctggcgatt atgagtcgct cgctctcacc
     2401 actgagctaa agggccggga gcagaataat aacggtccgt aattaatttc gcaataaaaa
     2461 aacccgctca gcggcgggtt gtagaaactc ttctaacgcc aggcataaaa cgcccatcat
     2521 tatgacgaat ttaccacaga ttccggaaaa atcaaccttg ttacctagtt acctttttta
     2581 actgccgctc agcccatgct tcttcaatat caaaccgggt caccagcgca tcataaaatt
     2641 tcttaactgt tttttcccat gacgcgcgtg ttatctggtt tgtcacctcg catatagcat
     2701 taaatgcctc cgttgatggt agtctttcat agccacgacc accacaacgc tggcagtctc
     2761 tgataacagg cataccacgt tttaccgact cttcacgatg aatggcgaca ccacgcccac
     2821 ggcaatcctt acaggcggtg gaaacctcac cctttccgcc acactccgga caggcaactt
     2881 ttaccacctc cctgactttt ttccattctt cccagtaaga cggatacaca cctttcgtac
     2941 actttgccca taccggcggc ttaccatccg gatactggat cttgtttgta aaaactatgc
     3001 tttcaataaa tttttcccca tagcaacaag ggcactgctt tttactcgct gcgctgcggg
     3061 cataatcctc aaaagcgtac gaagccataa tgcgcatcac taccggtttt atttctgccg
     3121 gaagttttct caacgccgcc acacgatcgc accgactgag tgcataatct gccagtaatt
     3181 ctgttgcccg cgccctgtca ttcatactga tgcccatttt ccccaggaac gcagaaaaac
     3241 ccatctcagc ccgattctgt gtcatgccct gcgcggccat cacatcagtg atactcagcg
     3301 catcttttga cgttgaggcc gatgcatcgg tcaggccagg ggattttggg gagtagtatt
     3361 tcggtaaatc ttccagtttc attttttgac ctgctcttaa tgcattatgg ggtaaatctt
     3421 cacccccaga cgtccaccag atactggctg accacgaacg atattgattt catcaaactg
     3481 ctcatcgtcc attaacactc ccgcatgcgt cagcgcatcc agcggtgctt tcaggatatt
     3541 gtccaggtcg cgacgacgct tatccggtgg ctctgcaatc acctttatcg ccagccttcc
     3601 ggacaggctt aatttcagcc gctgctggcg aacaataagc gccacagccc ggcgataacg
     3661 ctttccctcc tccgagataa aatatgtgct gccacggcgt cgccagtaag tgttcaccgt
     3721 cggcgggtaa ggtaaaacca aatctatgag catcagtcac ctcttttacc caagcacgcc
     3781 agttgcaaag gcgtgatcaa gaaaacgaaa aattaaatca acctgagaac catgcttttc
     3841 ttcgaacgcc agaggatccg catgaagctc gttgtgatgc tcccgacaca gcggtagcgt
     3901 gaaaatatcg tgagatttgg tccccattcc gccctgacca tgaccaatca ggtgatgggg
     3961 atcgtcggct ggcttaccac aacacgcaca cggctgtgtc ttcacccagc gtttgtattt
     4021 ctcgttaacc cagcggcgac gtttaggtcg tttcatgaaa gattccggag actcaggatc
     4081 aacggcaatg ctgaccaccg tcttttcctg ttgtgggttc tgttgctggt gggcgtgagg
     4141 cagcggcgca agattttttg tgcgctgttt cagtatgctg gtggcggtct gctctcccgg
     4201 tacgatgtcg ctttcacggt acattgagcg gattttttcc gcacgcaacc ccagcgaacg
     4261 acgtaatacc gcttccggta gcgcgtccgc cacctgattg cggaccgccc accaggataa
     4321 ttcagc
//
LOCUS       DAJTQY010000196         4279 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.198, whole genome shotgun sequence.
ACCESSION   DAJTQY010000196 DAJTQY010000000
VERSION     DAJTQY010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4279
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.198"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(<1..221)
                     /locus_tag="OO349_003959"
     CDS             complement(<1..221)
                     /locus_tag="OO349_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5868847.1"
                     /translation="MNNVFLYRMPVGIAGAVSRPQDLTVEPVVLKSDNAFAAYGLAGK
                     YDDDGFFVPLADGDTADKVKGIYVRPYPTT"
     gene            complement(225..1445)
                     /locus_tag="OO349_003960"
     CDS             complement(225..1445)
                     /locus_tag="OO349_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HCR5868848.1"
                     /translation="MKYFFETRLGETRYRLADGSLLCKDVPIGRTGKQLYGADDLPKL
                     KPDKFVEIVVTRSPEQVFHPATLASFEGMSITILHPEDENGNVRLVNPENWKVLAVGH
                     LQNVRRGTGEQSDLMLADLIVKDESAIQLIEDGLREVSCGYDAEYEQTEPCKAEQVDI
                     TGNHVALVPKGRAGNRCAIGDRDTMANQKKNWWNRMRAAIKTGDADTMNELVESAPAS
                     VTGDEGDLPQGVNLNINLSPQQPLPDKAPEMGGDPTGDSDDDLKTLLKALLAKLERNA
                     TGDNDNKPDDNPTGDGEDDEEETTITGDSAWRAEVIVPGIDLSRKMKPTAFKREVLAS
                     ADKTLVRQIVGDADIRKLPKQSVDMAFNAVSEVAKGRNTRATTGDAQRLNMGMTSIAS
                     LNKQNAEFWANRKG"
     gene            complement(1449..2192)
                     /locus_tag="OO349_003961"
     CDS             complement(1449..2192)
                     /locus_tag="OO349_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HCR5868849.1"
                     /translation="MTNRRSRQAEIRYRTSLRRIARAVGDIVNGRYDGSNDSITEIIA
                     ALERYSEIITPWATKVAENFTADLTRQNEKVWRQHSKNISRELRNLVESAPVGQVMQS
                     IIAEQVKYIKSLPLEAADRVYDIQNKAIEAVVTGGRAEQFAKEIASTGDVAKSRADLI
                     ARTELGRATGALDMARAIAIGSDGYIWRTADDGDVRDSHDHMKGKFVRWDSPPTLDGM
                     TGHAGELPNCRCYKEIVFVRVPFAMKRAA"
     gene            complement(2083..3549)
                     /locus_tag="OO349_003962"
     CDS             complement(2083..3549)
                     /locus_tag="OO349_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
                     /protein_id="HCR5868850.1"
                     /translation="MARNKQALRRTVQATADGYENFIARVGMQTPNQHSASTYRANFT
                     SRNRMLVEWSYRSSWIIGEAVDAIPDDMTRKGIRITSEIDAKDRGILESQLDELQIWD
                     ALNDVLKWSRLYGGAVGFIMIEGQAPMTPLRPETIGKGKFKGILPLDRWMVDPALTRR
                     IKDMGPDLGKPEFYDVVTTATGIPAWRIHHSRLIRFDGVTLPFQQKMTENEWGMSVVE
                     RIWDRLTAFDSATVGAAQLVYKAHLRTYSVEKLRELIALGGPAYEALLKNIDLIRQFQ
                     SNEGMTLMDSRDKFETHQYSFSGLDDILSQFAEQISGAVGIPLVRLFGQSPKGFSTGD
                     ADLANYYDRISSLQERRLRLPVRRILDIMHRSELGKPLPDDFTFEFNPLWQMSDVDRS
                     TVALNTTNAISTALGDGLMTLKAAMTDLRENSDVTGIGASITNEDIENAEDEAPPGIG
                     EPDDEPQEPSGGNPVSNQPTQDSEGGRRHRKWSLRWFK"
     gene            complement(3549..3782)
                     /locus_tag="OO349_003963"
     CDS             complement(3549..3782)
                     /locus_tag="OO349_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HCR5868851.1"
                     /translation="MDELISIDSRCPLLEKLKLELTTPHRDFDRNGRVMVESKKDLAK
                     REIPSPNVADAFIMAFAPIDTSLDIWEQLGRQA"
     gene            complement(3770..4249)
                     /locus_tag="OO349_003964"
     CDS             complement(3770..4249)
                     /locus_tag="OO349_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5868852.1"
                     /translation="MDAHKTLNFEPSGRKRIGFDVADSGTDKCANVYRHGSVVFWADE
                     WKAKEDELLKSCQRTYQAALEREADIVYDSIGVGASAGAKFSEINADRKSENAYARRV
                     NYQRFNAGAGVHEPDDEYNGIPNKDFFANLKAQAWWLTVSEIRLTPLTTENSILWMS"
BASE COUNT          908 a         1211 c         1062 g         1098 t
ORIGIN      
        1 gtggtcggat aagggcgcac gtagatcccc ttcaccttgt ctgcggtatc accatctgcc
       61 agcggcacga aaaaaccgtc atcatcgtat ttaccagcca ggccataggc agcgaaggcg
      121 ttatcggatt taaggaccac cggttcgacg gttaagtcct gcgggcgaga gacagccccg
      181 gcaatgccaa caggcatccg gtacagaaat acattattca ttttttaccc tttacggttt
      241 gcccagaatt cagcgttttg tttgttcagg gaagcgatac tggtcatgcc catgtttagg
      301 cgctgtgcat cgccggtggt ggcgcgggtg tttcgccctt tggcaacctc agacacggca
      361 ttaaacgcca tgtcgaccga ttgtttcggt aatttgcgga tatccgcatc accgactatc
      421 tggcgaacca gcgttttgtc agcagaagcc agaacctcgc gtttgaacgc ggtcggtttc
      481 atcttacggc tcagatcgat acccggaacg ataacttcgg cacgccaggc tgagtcacca
      541 gtaatcgtgg tttcctcttc atcgtcctcg ccgtcaccgg tcggattatc gtcaggctta
      601 ttatcgttat cgcccgtggc atttctttcc agcttagcca gcagggcttt cagtaatgtt
      661 ttgaggtcat catcactgtc gccggttgga tctccgccca tctctggtgc tttgtccggt
      721 agtggttgct gcggggacag gttgatgttg agattaacgc cctgcggcaa atccccctca
      781 tctcctgtaa ccgatgcggg agccgactcc accagttcgt tcatggtgtc ggcatctcct
      841 gtcttgatgg ccgcacgcat gcggttccac cagtttttct tttgatttgc cattgtgtct
      901 ctgtctccaa ttgcacaacg atttccggct ctgcctttag ggacaagagc cacatggttt
      961 ccggtaatat cgacctgctc ggctttacat ggctcggtct gctcgtactc cgcgtcatag
     1021 ccgcacgaca cttcgcgcag gccatcttcg ataagctgaa tggcgctttc gtctttgacg
     1081 ataaggtcag ccagcatcaa atcagactgc tcacccgtcc cgcgccggac attctggagg
     1141 tgcccgacag caagcacttt ccagttctcg ggatttacca gccgcacatt cccgttttca
     1201 tcttcaggat gcaggatcgt gatgctcatc ccttcgaatg aggcaagcgt ggccggatgg
     1261 aatacctgct caggagaacg cgtgacgact atttcaacga acttatcggg tttcagtttt
     1321 ggcaggtcat cagcaccata gagctgctta cctgttcgtc ctatcggcac gtctttgcac
     1381 agcaacgagc cgtcagccag gcggtatcgg gtttccccga gcctggtctc aaaaaagtat
     1441 ttcatgggtt atgctgccct tttcattgcg aatggaacgc gaacaaacac gatctcttta
     1501 tagcagcggc agtttggcag ctcgcccgcg tggccggtca tgccgtccaa agttggaggt
     1561 gaatcccagc ggacgaattt acctttcatg tgatcgtggg aatctcggac atcgccatca
     1621 tcggctgtac gccaaatata accatccgag ccaatagcta tcgctcgggc catatccagc
     1681 gcgcccgttg ctcttcccag ttccgttcgg gcaatcagat cggccctaga cttagcaaca
     1741 tcgccggtag atgcaatctc tttagcaaac tgctccgccc gaccaccggt gacaacagcc
     1801 tctatcgctt tgttctggat gtcgtacacc ctgtctgcgg cctcgagagg cagagatttg
     1861 atgtacttga cctgttcggc gatgatggat tgcatcacct ggcccacagg agcgctttcc
     1921 acaagattgc ggagctcgcg actgatgttc ttgctgtgtt gccgccaaac tttctcgttc
     1981 tgccgggtta ggtccgcagt aaagttttcc gcgacctttg tcgcccaggg ggtgatgatt
     2041 tcactgtaac gctccagcgc cgcaataatt tccgtgatac tgtcatttga accatcgtag
     2101 cgaccattta cgatgtctcc gaccgccctc gctatcctgc gtaggctggt tcgataccgg
     2161 atttccgcct gacggctcct gcggttcgtc atcaggttcg ccgatgccgg gcggcgcttc
     2221 atcttcggca ttctcgatgt cctcgttggt aatggatgcc cctatgccgg ttacgtcaga
     2281 attttctcgc aaatcagtca tagcggcttt cagtgtcatc agaccatcac ccagcgccgt
     2341 actgattgcg ttggtggtgt ttaacgccac cgttgagcga tcgacatcag acatttgcca
     2401 gagcgggtta aactcaaacg tgaaatcgtc cggcagcggc ttgccaagtt ccgaacgatg
     2461 catgatgtcc agtatccgcc gcaccggaag acgtaaacgc ctctcctgca acgaactgat
     2521 gcgatcgtaa tagttggcaa ggtctgcatc accggtagaa aatcctttcg gggactgtcc
     2581 gaacaaccgc accagtggga taccaacagc gccactaatc tgttctgcaa actgcgaaag
     2641 gatgtcatcc agaccactga agctgtactg atgggtttca aacttatccc gcgagtccat
     2701 gagcgtcatg ccttcattac tctggaactg tcgaatcagg tcgatattct tcagcaacgc
     2761 ttcatacgca ggaccaccaa gtgcgataag ctcgcgtagc ttctccacgc tgtaggtacg
     2821 caaatgcgcc ttgtagacca gctgcgccgc gccgacagta gcgctgtcga acgcggtaag
     2881 acgatcccag atacgctcta caaccgacat tccccattcg ttctcggtca tcttctgctg
     2941 aaatggcaac gtgacgccat caaagcgaat caggcgactg tgatgaatgc gccaggcagg
     3001 aattcccgtt gctgtggtca ccacatcgta aaactcaggt ttacccaggt ccggccccat
     3061 atctttaatg cggcgggtca gtgccgggtc gaccatccag cggtcgagcg ggagaatccc
     3121 cttaaacttg cccttaccga tggtttcggg tcgcagcggg gtcattggtg cctgcccctc
     3181 aatcatgatg aaacccaccg cgccgccgta gaggcgcgac catttcagca cgtcattcag
     3241 cgcatcccag atttgcaact catccagttg tgattcgaga atgccacgat cttttgcatc
     3301 aatttccgaa gtgatgcgaa tgcctttgcg ggtcatatca tccgggatag catcgactgc
     3361 ttcgccgatg atccaggatg aacgatagga ccattccacc agcatgcggt tacgactggt
     3421 gaaattagcc cggtaggtgg atgctgagtg ctggttaggt gtctgcatcc ctactcgggc
     3481 aataaaattc tcataaccat cagctgtggc ctgcacagtt cgccgcaggg cttgtttgtt
     3541 tcgtgccatc aggcctgtct ccccagctgt tcccagatat ccagcgatgt atcgattggc
     3601 gcgaaggcca taatgaatgc atcagcaacg tttggtgacg gtatctcgcg ttttgcgagg
     3661 tcttttttac tttcgaccat cacacgtccg ttacggtcga aatcacgatg aggtgttgtc
     3721 agttccagtt tcagcttttc aagcaacgga caacgagaat ctatgctgat cagctcatcc
     3781 acaggatact gttctccgtt gttaatggcg ttaaacgtat ttctgaaacg gtcagccacc
     3841 atgcctgagc cttaagattt gcgaaaaagt ctttgttggg gatgccgttg tactcgtcat
     3901 ctggctcatg cacaccagca ccggcgttaa acctctggta attcacacgt cgcgcgtatg
     3961 cgttctcgct cttccggtca gcgttaattt cagagaattt agcaccggca gacgcaccaa
     4021 caccgataga gtcgtaaaca atatctgctt cacgctccag cgccgcctga taagtacgct
     4081 ggcagctctt cagtaattca tcttctttgg ccttccattc gtcggcccag aaaacaacgg
     4141 atccgtgacg gtaaacgtta gcgcacttat ctgtaccact gtcagccacg tcaaagccaa
     4201 tacgctttct tccactgggt tcgaaattta acgttttgtg cgcatccact gcggcttcta
     4261 tccaagacag tttgatgat
//
LOCUS       DAJTQY010000197         4242 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.199, whole genome shotgun sequence.
ACCESSION   DAJTQY010000197 DAJTQY010000000
VERSION     DAJTQY010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4242
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.199"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            29..910
                     /gene="yddG"
                     /locus_tag="OO349_003965"
     CDS             29..910
                     /gene="yddG"
                     /locus_tag="OO349_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HCR5868853.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYTAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            1165..2265
                     /gene="ompC"
                     /locus_tag="OO349_003966"
     CDS             1165..2265
                     /gene="ompC"
                     /locus_tag="OO349_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCR5868854.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGLATYRNYDF
                     FGLIEGLNFAAQYQGKNERTDNSHLYGADYTRANGDGFGISSTYVYDGFGIGAVYTKS
                     DRTNAQERAAANPLNASGKNAELWATGIKYDANNIYFAANYDETLNMTTYGDGYISNK
                     AQSFEVVAQYQFDFGLRPSLAYLKSKGRDLGRYADQDMIEYIDVGATYFFNKNMSTYV
                     DYKINLIDESDFTRAVDIRTDNIVATGITYQF"
     gene            2429..2914
                     /locus_tag="OO349_003967"
     CDS             2429..2914
                     /locus_tag="OO349_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HCR5868855.1"
                     /translation="MIMTNINTACVKNNASYQLNNALPNKETISSNFCERLEQWGNKS
                     VNNGEERAIAVERIKEAYNSNMASLDLSYLDLSELPPIPSTVNTLNLENNCLTCLDLA
                     DNASLVNINLSVNKINTITFPKEPKLENIYIDHNNLESLDLKNQHSLVNLEAQNNSLK
                     K"
     gene            3024..4013
                     /locus_tag="OO349_003968"
     CDS             3024..4013
                     /locus_tag="OO349_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HCR5868856.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQGRNVSITHISMN
                     NNALRNIDIDRLSSVTYFSAAHNQLEFVQLESCEWLQYLNLSHNQLTDIVAGNKNELL
                     LLDLSHNKLTSLHNVLFPNLNTLLINNNLLSEIKIFYSNFRNVQTLNAANNQLKYINL
                     DFLTYLPSIKSLRLDNNKITHIDTNNTSDIGTLFPIIKQSKNLNFLNVSGKNN"
BASE COUNT         1316 a          875 c          805 g         1246 t
ORIGIN      
        1 taacggcaag agagacaaaa cagcgagcat gacacgacaa aaagcaacgc tcatagggct
       61 aatagcgatc gtcctgtgga gcacgatggt aggattgatt cgcggtgtca gcgaggggct
      121 cggcccggtc ggcggcgcag ctgctatcta ttcattaagc gggctgctgt taatcttcac
      181 ggttggattt ccgcgtattc ggcagattcc gaaaggctat ttactcgccg ggagtctgtt
      241 attcgtcagc tatgaaatct gtctggcgct ttccttaggg tatgcggcga cccgtcatca
      301 ggcgattgaa gtgggtatgg tgaactatct gtggcccagc ctgacaattc tctttgccat
      361 tctgtttaat ggtcagaaaa ccaactggct gattgtacca ggattattat tagccctggt
      421 cggcgtctgc tgggtgttag gcggtgagaa tgggttacat tatgatgaaa tcatcaataa
      481 tatcaccacc agcccattga gttatttcct ggcgtttatt ggtgcgttta tctgggcagc
      541 ctattgcaca gtaacgaata aatacgcacg cggatttaac ggaattaccg tttttgtcct
      601 gctaacggga gcatgtctgt ggatttacta ttttctcacg ccacaaccag aaatggtatt
      661 tagcacgccc gtcatgatta aactcatctc tgcggcattt accttaggat ttgcttatac
      721 tgcatggaat gtcggtatat tgcatggcaa tgtcaccata atggcggtag gctcgtattt
      781 tacgcctgta ctttcctcag cgcttgcagc cgtgctgctc agcgccccgc tgtcgttctc
      841 gttctggcaa ggcgcactaa tggtctgcgg cggttccctg ctctgctggc tggcgacacg
      901 tcgtggttaa aataattgcc gggtctacgg cccggcagtt aatcagataa attccgacaa
      961 tattaaaaaa taaatttcaa ataccagtaa ttcaccttat atctcatttc gacttcgatc
     1021 acatatcgat aaataatatt attctatttt tctcattgtt tttaatcacc attctatttt
     1081 aaaaacagta tgtcatttat aaatgacgcc gtttaaaatt cgtttagaaa atacgtttaa
     1141 cgataattaa aaggattctg ttaaatgaaa ttaaaaatag ttgcggtggt tgtaactggt
     1201 ttgttagctg cgaacgtagc acacgctgcc gaagtctata acaaggatgg taataaactc
     1261 gacctttatg gcaaggttac cgctctacgt tattttactg atgataagcg tgacgatggt
     1321 gataaaactt atgcccgtct cggctttaaa ggagaaacgc aaatcaatga tcaaatgatt
     1381 ggttttggtc actgggaata tgattttaaa ggctataacg atgaagccaa cggctcgcgc
     1441 ggcaacaaga cccgtctggc ctatgctggt ttaaaaatta gtgaatttgg ctctctggac
     1501 tatggtcgta actacggtgt cggctacgac attggttcat ggaccgatat gttgccagaa
     1561 tttggtggcg atacctggag tcagaaagat gtcttcatga cataccgtac caccggtcta
     1621 gcaacctatc gcaactacga tttctttggc ttaattgaag ggctgaactt tgccgcgcaa
     1681 tatcaaggca aaaatgaacg tactgacaac agtcatcttt atggtgctga ctacacgcgt
     1741 gccaacggtg acggtttcgg tatctcctca acttatgttt atgatggctt tggtatcggt
     1801 gcggtatata ccaaatccga tcggacaaat gcgcaggaaa gagccgcagc taatcctctc
     1861 aatgcctccg gtaagaatgc agaactatgg gccacaggta taaagtatga tgccaacaac
     1921 atctactttg cagctaatta cgatgaaaca ttaaacatga ccacctatgg ggatggttat
     1981 atctctaaca aagcacaaag ttttgaagtg gtggcgcaat atcaattcga cttcggcttg
     2041 cgcccatcac tcgcttacct gaaatcgaaa ggcagagatc tgggccgcta cgccgaccag
     2101 gacatgattg agtatatcga cgttggtgcg acgtatttct tcaacaaaaa tatgtcgacc
     2161 tatgttgatt ataaaatcaa cctgattgat gaaagcgact ttacccgtgc cgtagatatt
     2221 cgcaccgata acatcgtcgc tacgggcatt acctatcagt tctaacgtcc tctcctgacc
     2281 agcccttctc tgggctggtt tttgatttaa gtgctgctgt atgactcctt aagtaaaaat
     2341 gagcaattct tgatatttct ggtcattcac ctcaattaat attattccat atcctccggg
     2401 gacatcttac tataatattt attgatgagt gattatgacc aatattaata cagcttgtgt
     2461 aaaaaataat gccagttatc aacttaataa cgcattacct aacaaagaaa ccatctccag
     2521 caatttttgt gaacgactag aacaatgggg taataaatcg gttaataatg gtgaagaaag
     2581 agcaattgcc gtggagcgaa ttaaagaagc ttacaattcg aacatggcat ctcttgattt
     2641 gtcatatctt gatttgagtg aattaccgcc tatcccctcg acagttaata cgttaaatct
     2701 ggaaaacaat tgtctaactt gtcttgactt agctgacaat gccagtctcg ttaatatcaa
     2761 cctcagcgtt aataaaatta acacgataac ctttccaaaa gaaccaaaac tggaaaacat
     2821 ttatattgat cataacaatc tggaaagttt agatttaaaa aatcagcatt cattggttaa
     2881 tctggaagcg caaaataata gcctaaaaaa atgatatttc tgatagttat aaactgaaat
     2941 ttcttaatct tgattataac aagctagcat cgctggatgt ctcccggcaa gaatctctga
     3001 ttgagttaag tgcccatcac aatatgatca ctgaccttat attacacaat catcccagga
     3061 tgaaaacaat cactttaaac gacaaccata ttgcacattt aaacgccaaa aacactacaa
     3121 aactggaata tttaaactta agcaataaca atttactgcc aaccaatgac attgatcaac
     3181 taatatcatc aaaacatctt tggcatgtat tagttaacgg catcaacaat gatccacttg
     3241 cacaaatgca gtactggact gcagtaagaa atataattga tgacactaat gaagtgacca
     3301 ttgatttatc aggacttaat ttaaccactc aaccaccagg gctgcaaaac ttcacctcta
     3361 tcaatcttga taataaccaa ctcacacatt ttgatgcaac caactacgat agactcgtaa
     3421 agctaagtct gaatagtaat gctcttgagt caataaattt tcctcaaggc agaaatgtaa
     3481 gtattacaca tatatctatg aataataatg ctctcagaaa tattgatata gataggcttt
     3541 catcagttac ttattttagt gcggcacata atcaactaga gtttgtgcaa ttagaatctt
     3601 gcgaatggct gcaatacctg aatctcagcc ataatcaatt aactgatatt gttgcaggta
     3661 ataaaaatga actcttactg ctggatctat cccataataa actaacaagt ttacacaatg
     3721 tcttatttcc caacttgaat acgttactta ttaacaacaa tttgctttct gaaattaaaa
     3781 tattctatag caacttccgc aatgttcaga cattaaacgc tgctaacaac cagttgaaat
     3841 atataaatct tgatttcctg acttatcttc catctatcaa aagtttaaga ctggacaata
     3901 ataaaataac ccacattgat actaataata catccgatat tggaacttta ttccccataa
     3961 taaaacagag caaaaactta aattttttaa atgtttctgg gaagaacaat tgacctacta
     4021 tgcagctcat gttatttaat ttgttttccc cagcacttaa gcttaatact ggcctggcaa
     4081 ttctttcgcc tggtgcattt gaagttcact ctgacggaat agatgcggat aacgaattgt
     4141 ttcactatac tattaaaaaa gcatataccc catataatat acacacttac aagacagagg
     4201 aagttgtaaa ccagaggaat ataaaagtta aaaacatgac ct
//
LOCUS       DAJTQY010000198         4183 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.200, whole genome shotgun sequence.
ACCESSION   DAJTQY010000198 DAJTQY010000000
VERSION     DAJTQY010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4183
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.200"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OO349_003969"
     CDS             complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OO349_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR5868857.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(324..399)
                     /locus_tag="OO349_003970"
     tRNA            complement(324..399)
                     /locus_tag="OO349_003970"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(364..366),aa:Thr,seq:ggt)
     gene            complement(406..480)
                     /locus_tag="OO349_003971"
     tRNA            complement(406..480)
                     /locus_tag="OO349_003971"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Gly,seq:tcc)
     gene            complement(597..681)
                     /locus_tag="OO349_003972"
     tRNA            complement(597..681)
                     /locus_tag="OO349_003972"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(645..647),aa:Tyr,seq:gta)
     gene            complement(690..765)
                     /locus_tag="OO349_003973"
     tRNA            complement(690..765)
                     /locus_tag="OO349_003973"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(730..732),aa:Thr,seq:tgt)
     gene            1127..2077
                     /gene="coaA"
                     /locus_tag="OO349_003974"
                     /gene_synonym="panK"
     CDS             1127..2077
                     /gene="coaA"
                     /locus_tag="OO349_003974"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HCR5868858.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHVVEEVRLRK"
     gene            complement(2106..3071)
                     /gene="birA"
                     /locus_tag="OO349_003975"
     CDS             complement(2106..3071)
                     /gene="birA"
                     /locus_tag="OO349_003975"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HCR5868859.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGEMLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQFLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3068..4096)
                     /gene="murB"
                     /locus_tag="OO349_003976"
     CDS             complement(3068..4096)
                     /gene="murB"
                     /locus_tag="OO349_003976"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HCR5868860.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1081 a         1039 c          929 g         1134 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcgcgagc agcaccgccg taggttttag ccagtacggt agtgattgca
      121 gcggtcagcg ttgttttacc atggtcaacg tggccgatag taccgacgtt aacgtgcggt
      181 tttgtacgtt caaacttttc tttagacatc gattgtccct ctaagacacg gataaatcgg
      241 tgatatcacc acatcaacca ggcaacatgc ccgacttgtt gaatgcaata aacagaagga
      301 aaaaacaggg aggagaaaag aagtggtgct gataggcaga ttcgaactgc cgacctcacc
      361 cttaccaagg gtgcgctcta ccaactgagc tatatcagca catcttggag cgggcagcgg
      421 gaatcgaacc cgcatcatca gcttggaagg ctgaggtaat agccattata cgatgcccgc
      481 atcctagaac tcggctacct gattttcatt ctgcactgaa tatcgagaga agctctcttt
      541 attcgagccg gtaagtgaac ttatcgtctc gggctacgcc atcgcgtggc cgaaattggt
      601 ggtgggggaa ggattcgaac cttcgaagtc tgtgacggca gatttacagt ctgctccctt
      661 tggccgctcg ggaaccccac cggacttgat ggtgccgact accggaatcg aactggtgac
      721 ctactgatta caagtcagtt gctctaccta ctgagctaag tcggcatcaa gtagcgcgca
      781 ttctatggag acatgcgagt tcatgcaact aaaaaattgc ataatttgtt ttattggtca
      841 cattttatgc tacacgatga agaaacagcc gaacggaggg cagattagca cactttttca
      901 acatcattgt gctcaacaat gcgctcctgc taaaccataa ttctttttat cagatggaat
      961 atctgtcaca ttgcttttca acgatagctt cctggcagag attttttctt attattcctc
     1021 cccatctggt gttaccctcc tgcccattaa cccattcaac agaactgtga cgcgccatgg
     1081 caaatatcgc tttgccgata gagctatgac cgccagaaac atgcttatga gtataaaaga
     1141 gcaaacgtta atgacgcctt acctacagtt tgaccgcaac cagtgggcag ctctgcgtga
     1201 ttccgtacct atgacgttat cggaagatga gatcgcccgt ctcaaaggta ttaatgaaga
     1261 tctctcgtta gaagaagttg ccgagatcta tttacctttg tcacgtttgc tgaacttcta
     1321 tataagctcg aatctgcgcc gtcaggcagt tctggaacag tttcttggta ccaacgggca
     1381 acgcattcct tacattatca gtattgctgg cagtgtcgcg gtggggaaaa gtacaaccgc
     1441 ccgtgtattg caggcgctat taagccgttg gccggaacat cgtcgtgttg aactgatcac
     1501 tacagatggc ttccttcacc ctaatcaggt tctgaaagaa cgtggtctga tgaagaagaa
     1561 aggcttcccg gaatcgtatg atatgcatcg cctggtgaag tttgtttccg atctcaaatc
     1621 cggcgtgcca aacgttacag cccctgttta ctcgcatctt atttatgatg tgatcccgga
     1681 tggagataaa actgttgttc agcctgatat tttaattctt gaagggttaa atgtcttaca
     1741 gagcgggatg gattatccac acgatccaca tcatgtattt gtttccgatt ttgtcgattt
     1801 ttcgatatat gttgatgcac cggaagactt acttcagacg tggtatatca accgttttct
     1861 gaaattccgc gaaggggctt ttacagaccc ggattcctat tttcataact acgcgaaatt
     1921 aactaaagaa gaagcgatta agactgccat gacattgtgg aaagagatca actggctgaa
     1981 cttaaagcaa aatattctac ctactcgtga gcgcgccagt ttaatcctga cgaaaagtgc
     2041 taatcatgtg gtagaagagg tcagactacg caaataattt gcaggggagc gaatactccc
     2101 ctttcttatt tttctgcact acgcagggat atttcaccgc ccatccaggg ttttattatt
     2161 ccatcctgct caagtaataa agccccctgt ttgtctattc cgcgtgaaat gccaaatatt
     2221 tctttatcac caatgataag tttcactggg cgattaataa aattatccag cttttcccag
     2281 cgcgacagat aaggtgccaa tccttcttgt tcgaagagtt ccaacgcagc acgtaattca
     2341 cgtattagca tggccgccaa cgtattacga tcaagattga tccccgcttc ctgcagcgtg
     2401 atccacccct gattaacgac actctcttca acacggcgca ttgccatgtt gatcccggct
     2461 ccaatgacta tttgcgccgc atcgccagtt ttgccagtca gctccacaag aatgcctgcc
     2521 agcttgcgat cctgcagata gaggtcatta ggccatttga cacgaacttt atctgctccc
     2581 agctttcgta atacttccgc catcacgata ccgataacca gacttaaacc aatcgccgcc
     2641 gccgggcctt gttccagacg ccagaacatc gacaaatata agtttgcgcc aaaaggcgaa
     2701 aaccatttcc gcccccggcg accacggcca gcctgctggt attctgcaac acaggcatcg
     2761 cccgatttaa gctctccgat acgatcaaga aggaactgat tcgtggagtc aataactggc
     2821 agcacggcta cactaccgcc atccagctga cccaatatct gttcagcatt aagtaactgg
     2881 atgggctcag gcaggctgta tcctttaccc ggaacggtaa agacatcaac gccccagtca
     2941 cgcagtgtct gaatgtgttt attaatagcc gcccggctca ttcccagcat ttcacccaac
     3001 tgctcgccag agtgaaattc accgttcgct aacagggcaa tcaatttcag tggcacggtg
     3061 ttatccttca tgaaattgtc tccactgcgc tcacttcacc tgatgcacca ataaagcgga
     3121 cttcaggctc aagccagaca ttaaattttt cacccacttt ttgtcttaca tggtgtgcca
     3181 gttgcaccac atcttcgctt tttgcattgt cttcattaat gagaactaac gcctgttgac
     3241 ggtgcaccgc agccccaccc atttgcatcc cttttagctg gcactgatca ataagccaac
     3301 ctgctgccag ttttactgaa ccacccgcct gggggtaatt tggtgctgtt ggaaattgcg
     3361 ccagtaatgc tttagccgtt tcggcagata caacagggtt tttgaagaaa ctaccggcat
     3421 tgccattcac ttttggatca gggagtttgg tggtgcgcat atgacacacc gcattaaata
     3481 cttgctgtgg cgttactgtt gtaggatcca gacgagttaa gtcaccatac gttagtacag
     3541 gttgccactc ttttggcaga cgcagaccta cggctacaat ggcgaagcga tcctggtatt
     3601 catgtttaaa aatactgtcg cgatagccaa aacggcactc tttggcagtt aagcgcactt
     3661 gcttgcctgt cgccagttca acacaatcaa cataagcgca aactcgctgt aattctacgc
     3721 cataagcacc aatattctgg ataggtgatg agccgacaca accaggaatt aatgccagat
     3781 tttccagacc aggcatacct tcctgcaaag tgtattttac caggcgatgc cagttttctc
     3841 cggctcctac atgtaaatac cacgcatcag gttcatcatg aatttcgata cctttgatcc
     3901 ggttgatgat caccgtgccg cgatagtctt ccagaaaaag tacattactt ccttcaccca
     3961 gaataagaac gggttgtcct tctgcggttg catgctgcca ggcattgagt aattgttgtt
     4021 cgtcttcggc acatacaatg tgctgagcat tatgatcaat gccaaatgtg ttccagggtt
     4081 ttaaggagtg gttcatagct gctttcctga tgcaaaaacg aggctagttt accgtatctg
     4141 tggggggatg gcttgtagat atgacgacag gaagagtttg tag
//
LOCUS       DAJTQY010000199         4168 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.201, whole genome shotgun sequence.
ACCESSION   DAJTQY010000199 DAJTQY010000000
VERSION     DAJTQY010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4168
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.201"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(<1..334)
                     /gene="flhe"
                     /locus_tag="OO349_003977"
     CDS             complement(<1..334)
                     /gene="flhe"
                     /locus_tag="OO349_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HCR5868861.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     ARQPASGLMTLVAWRYQLIGPTPAGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGG"
     gene            complement(334..2411)
                     /gene="flhA"
                     /locus_tag="OO349_003978"
                     /pseudo
     CDS             complement(334..2411)
                     /gene="flhA"
                     /locus_tag="OO349_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(2404..3551)
                     /gene="flhB"
                     /locus_tag="OO349_003979"
                     /pseudo
     CDS             complement(2404..3551)
                     /gene="flhB"
                     /locus_tag="OO349_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
     gene            3787..>4168
                     /locus_tag="OO349_003980"
     CDS             3787..>4168
                     /locus_tag="OO349_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCR5868862.1"
                     /translation="MEWQQLLNCNRRKDNLKKTNIAFLHPLASGRQEIERDYDRLLFA
                     APTRRLADKTQVFPLDQNDSIRTRLTHSHEVANFARGIGMRLAFEMKEHIFGEIPTGI
                     VVERDVPALLAAIGLAHDLGNPPFG"
BASE COUNT         1037 a         1208 c         1023 g          900 t
ORIGIN      
        1 caccgcctgg cacttcccag ataaatcgca acggttctgc tgccgctata ccggaaaagg
       61 ccacggtggt accgctctgc ccctctaatt cgacacaacg agattgcgaa cacaagcgaa
      121 cccgcagtcc tgcaggtgtc gggccgataa gctgataacg ccacgccacc agcgtcatca
      181 atcctgaagc aggttgtcgc gcagaaagag gtgcagacga catcgactca ccgcgatgat
      241 ttagcgtaat tccgacacta cttgcctgcc acatcccctc cccggcggct tgcaccagca
      301 gcggaaacaa taatatcgct aataaggttc tcatcattta ccgccaattg tcgccgtcat
      361 gcggatatgt cggttatcag acagttccag attcgacagg accactaact gcggcaagct
      421 gcggcgcagg aagcgagaca ataatggtcg cagcgcgtgg ttcaccaaca atactggcgg
      481 cgcacccagc atctcctgac gggatagagc ttcctgagtt tgcgccagta aacgatccgc
      541 cagccctggc tccagtcctc ccccgccctg cagcgcctgt agtaacaaac gttccagcgg
      601 tgtatcgagg ccaataacat ggacttcatc tttgccagga aaccactgct gggtaatcgc
      661 ccgtcccaac gccacgcgca cgacggcggt taattcatgt ggatcgcttt ggatgggcgc
      721 atgttccgcc agcgtttcga gaatggtgcg catatcgcga atcggtactt tttcatcgag
      781 gagattttgc agcactttat gcagtgtggt gagcgtgacg acgccaggaa cgagatcttc
      841 cgtcagcttt ggcatctcct gggcgacgcg atccaacagc tgttgcgcct cctgacgacc
      901 aaacagctct gcggcatgct ggctaatgag gtggttaaga tgcgttgcta ccaccgtgct
      961 ggcctcaacc actgtgtacc cctgaatctg cgcctgttct tttagcgcac tttcaatcca
     1021 gatagcattc aggccaaatg ccggatcgac ggtcgcctca ccaggtaacg tcccggcagc
     1081 ggttccaggg ttaatcgcca gccagcgccc cggataagca tcaccactgc caatctccac
     1141 gcctttcatc aaaatgcgat agcgggcagg ttgcagatcc atattgtcgc gaatgtgcac
     1201 cactggcggc agaaatccca tctcctgggc aaatttcttg cggatactgc gtatacggcc
     1261 caacaactca ccatcctgct ggaaatcgac catcgggatc agtcgataac ccacttccat
     1321 tcccagagaa tcttccagtt gtacatcgtt ccacgtcgct tcgacaacgg tattattctc
     1381 tgccattttt accggtttgg gttcggcagg cgctttttgt tcgcgtccgc gtatccacca
     1441 ggccagcccc agcaatccgg cagtgaacag caaaaatacc aggttcggca ttccaggcac
     1501 caggccgagt aaaccgagca cggcggcgct taacaacata acgcttgggt tactgaaaag
     1561 ctgattcacc atctgctcgc caacatcctg atcggtgctg acacgcgtaa cgatgccccc
     1621 cgcggcggta gaaatcacca gcgccggaat ttgtgccacc agaccgtcgc caatggtcaa
     1681 tagcgtataa ctttccgccg cgtgtcccat gctcatgcca gttgcagcac gccgaccagc
     1741 aacccgccga caacgttaat gaccatgatg aggatcccgg caatggcatc gccgcgaaca
     1801 aacttacttg ccccgtccat tgagccgtaa aaatcggctt cctgagtcac ttcggagcgg
     1861 cgttttttcg cctcatcttc accaatcaat ccagcgttaa ggtcggcgtc aatcgccatc
     1921 tgcttacccg gcataccatc gagaacaaag cgcgcaccca cttctgctat acgcccggca
     1981 cctttggtaa tgaccataaa gttgatgatc acgagaatga caaacaccac gataccgata
     2041 gcgaaattgc caccaacgag gaagtgaccg aacgcttcga ccaccttccc agccgccgcc
     2101 gcgccggtat gcccttccat caaaatgata cgggttgaag ccacgttaag tgccagacgc
     2161 aacaacgtag taaacaacag aatggtcgga aacgcagcaa actcaagcgt gcgctgggta
     2221 aacatcgcca ccagcaacac catgatcgac aaggcaatat tgaaggtaaa caacaggtcg
     2281 agtatgaatg cgggcagtgg cagcaccatc atcgacaaga tcaacaggat caaaatcggt
     2341 ccggcaagga tctgccatta tgtcgatttc aggtttgcgg gcaggcgcag catcgcggcc
     2401 agattactca tgggtcggtt tctcgttaat aaaatccagg gctgccggca ccggaagatg
     2461 agtaggtgta cagggcgctg tccaccagcc agacgccagc gtttcagttg ccagacccag
     2521 gccagcactt ccgccaccgc ggcgtacagt tgacccggga tttgttgacc aatctccgca
     2581 tgtcgataca gcgctcgcgc cagcggcggc gcttcaagcg tcgggacgtt attttcagcg
     2641 ccaatttcac gaatgcgcag cgcgaccagc cctgcacctt tagcgaccac tttcggtgcg
     2701 ctcattttgt tttcgtcata ctgcaacgct accgaatagt gggtcgggtt attgacaatg
     2761 acatccgctt tcggcacatc ggccatcatc cgacgccgtg cggcagctcg ctgcatctga
     2821 cagatccgtc ctttaacatg ggggtcgcct tcgccttgtt tgaactcatc acgaatatcc
     2881 tgccgcgaca ttcgcagctt tttcaggtgg ctgaagattt ggaaaaagac gtcaaatccc
     2941 accattggaa tgacaccaag caccaccagc agtgcgcata gccctaccaa atccatcgca
     3001 ttacccatgg cggtaatcgg tgactcggcc attaagcgca tcatctgcgg ccagtgatgc
     3061 cagagaaaaa accccgtcac gctgccaacc aggatggttt tcaaaattgc cttaagcaac
     3121 tccgcgctag tctgtgccga gaatatccgt ttaatgcccg gtagcgggtt gagtttggaa
     3181 aacttcggct gcaaggattt gccgctaaat accagccccc ccagcataac cggagaaatg
     3241 agcgccacca gcaccacgcc gctaatcagc ggtagcagcg ccagcatggc ttctctgatc
     3301 agcagaataa tttgcccgag gatcagattc gggtctttga taatactgtg atcaaaatgc
     3361 agcccagcgg agagcatgcc cgacaatcga cgggccagcg acacaccgcc aaaccagata
     3421 acactaacgc ccaccagcaa aatcagcagt gaggtcagtt cacgggaacg cgggatttgc
     3481 ccctcttccc gcgctttttc tagtcggtga ggtgtggggg cttctgtttt gtcgtcgctc
     3541 tcgtcagaca cgtcgccaat cctggaaaga gtattaagcg taaatgatgc cagagcgcaa
     3601 agcgttcaat ggtttaagta aggggcaaaa caggcgggat ttagggcgtt tatgcaaaaa
     3661 taggttcagt tgcagaacgt tttgcgaacc attttccagt tctgatattt agccatttag
     3721 cctatagaat cgcactccgc ctctctgtta gtttgctaat aattaaatta ttacaaaggt
     3781 gatcaaatgg aatggcaaca attgcttaac tgcaaccgtc ggaaagataa tctaaaaaaa
     3841 accaacatag catttttaca tccccttgct agcggaagac aagagatcga gcgggattac
     3901 gaccgattac tttttgctgc accaactcgt cggcttgcgg ataaaacaca agttttcccg
     3961 ttagatcaaa atgacagcat acggacgagg ctgactcatt ctcacgaagt agcaaacttt
     4021 gctcgcggga taggaatgag gctcgctttc gagatgaagg agcacatctt tggcgaaata
     4081 cccacgggga ttgtcgttga acgcgacgtt cctgctctgt tagcagcgat cggactggca
     4141 cacgatctcg gtaatccacc atttgggc
//
LOCUS       DAJTQY010000200         4128 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.202, whole genome shotgun sequence.
ACCESSION   DAJTQY010000200 DAJTQY010000000
VERSION     DAJTQY010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4128
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.202"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            1..440
                     /gene="tnpA"
                     /locus_tag="OO349_003981"
                     /pseudo
     CDS             1..440
                     /gene="tnpA"
                     /locus_tag="OO349_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459923.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(558..1502)
                     /gene="hypT"
                     /locus_tag="OO349_003982"
                     /gene_synonym="qseD"
     CDS             complement(558..1502)
                     /gene="hypT"
                     /locus_tag="OO349_003982"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001333359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HCR5868863.1"
                     /translation="MVMKNIETKWLYDFLTLEACRHFSQAAKERNLSQPAFSRRIKAL
                     EAAIGVVLFDRTTTPLQLTEEGKLFHSQTRSLLQQLECNLGELNGQSLLGVPNIKIAA
                     AHSLSLSVLPKLVHSLTAYGGEFVYHVEAIDVVQAVNTLREGKSDFIISFRDEDLMQS
                     PFCCLKLFESELYPVCAADAQGHPVFDITQPQVPLLNYTATSYMGRLVNRHLAEVGGI
                     TGRTIFISSMSELLKNMALNGYGIAWLPIWSIVDELQTKRLICLDAAKLTVPIQAYIY
                     RMNTRLNRTAENLWRILQEHMPDDLIQQISMEEPARRN"
     gene            1847..2566
                     /locus_tag="OO349_003983"
     CDS             1847..2566
                     /locus_tag="OO349_003983"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016156986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HCR5868864.1"
                     /translation="MKGLIGVLGGMGPAATVDLFNKFVTFTAAQCDQEHIPLIISSIP
                     DIPDRTDALMHHGRSPLPVMRDYMHKLEDAGAECIVIPCNTAHYWFKELKDACHIDIL
                     SIVETTINEVRACGKTRIGLLATNATLYMGLYQKGIESLGFTCVSPDADGQKKVMESI
                     YSLKAGNIAHAQKLMNEQAEILFSRGAEILVLGCTEIPVILAQAVKEQPSRYIDSTAS
                     LVRAGIKWYENRVGKHYLLTQ"
     gene            2632..3930
                     /locus_tag="OO349_003984"
     CDS             2632..3930
                     /locus_tag="OO349_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HCR5868865.1"
                     /translation="MVWLEIIVVLGAIFFGIRQGGIGIGLCGGLGLAILTLGFGLPMG
                     SPPVDVILIIMTVVVAASALQAAGGMDYLVRVAGNFMRRNPKYINIIAPIITWSMTIM
                     AGTGFIVFSTLPVIAEVAKESGIRPSRTLAGSVVASQVAISGSPISAAMAAMLTIMEV
                     NGVSFIQVMSVCLPTSFAAAMVAAFIASRQGCELQDDEVYLERLQKGLVQKYENNSSI
                     KPGATLSVGLFLLATVAIVILAAFPQLRPGFDIGKPMETRDIIIICMLSAACLMVVLC
                     KTATDAIILTSTFRAGMSSLAVILGIVTLGTTFIDAHLTEIKDIAGDILQAYPMLLAL
                     VLFGTCALLYSQGATTPLIIPLAVALDVPTWAILASYVAVTGVFVLPTYPTSLAAMEF
                     DTTGTTRVGKYVLNHPFMLPGLGGIIAGVALGFVIAPMII"
BASE COUNT         1043 a          895 c         1018 g         1172 t
ORIGIN      
        1 agcgcacacc cgatggaact gtaaatatca catagttttt tgcgccaaaa taccgaagac
       61 aggtgttcta cagagagaag cgtagagcaa caggcagtat tttgagaaag ctgtgtgagt
      121 ggaaaagcgt acggattctg gaagctgaat gctgtgcaga tcatatccat atgcttgtgg
      181 agatcccgcc caaaatgagc gtatccggct ttatgggata tctgaaaggg aaaagcagtc
      241 tgatgcttta cgagcagttt ggtgatttga aattcaaata caggaacagg gagttctggt
      301 gcagggggga ctacgtcgat acagtgggta agaacacggc gaagatacag gattacataa
      361 agcaccagct tgaagaggat aaaatgggag agcagttatc gattccctgt ccgggcagcc
      421 cgtttacgga ccgtaagtaa cgaagttgga tgcaaatgtc agatcgtgtg cgcctgttag
      481 ggcgcggctg gtaagagagc cttataggcg catttgaaaa acctccggct atgccggagg
      541 atatttattg cacgtcgtca attgcggcgt gctggctctt ccattgatat ctgctggatt
      601 aaatcgtctg gcatatgttc ctgtaaaata cgccagaggt tctcggcggt tctattcagt
      661 cgggtgttca ttcggtaaat ataagcctga atggggactg tcaatttagc agcatcaagg
      721 catatcagac gtttggtctg tagttcatcg acgatcgacc agatcggtaa ccaggcaatg
      781 ccgtatccgt tgagcgccat attctttaat aactcgctca tcgaagagat aaatatcgtg
      841 cgcccggtaa tacctccaac ctctgccaga tgacgattga ccaacctgcc catataagat
      901 gtggcggtat agttgagcaa aggaacctgg ggctgagtga tatcgaatac agggtgcccc
      961 tgtgcgtcgg cggcacatac cgggtacaac tcagattcaa acagtttcag gcagcaaaaa
     1021 ggcgattgca taagatcttc atcgcgaaac gaaataataa agtcactctt gccttcgcgt
     1081 aacgtattta cggcctggac aacatcaatg gcttcaacat gatagacaaa ttctccgcca
     1141 taggcggtaa gtgaatgcac cagtttgggt aatacgctca gggatagtga gtgcgcggct
     1201 gcaattttaa tgttgggaac gccgagtaaa ctctggccgt ttaactcccc caaattgcat
     1261 tcaagctgct gtaataagct gcgagtctgg gaatgaaaga gcttgccctc ttcggttaat
     1321 tgtaaagggg tggtcgtgcg atcgaataaa acaacgccga tcgctgcctc cagcgcctta
     1381 attcgtcggc tgaatgcggg ttgcgaaaga ttacgctctt ttgccgcctg agaaaaatgg
     1441 cgacaggctt ccagtgtcag gaaatcgtat aaccattttg tctcgatatt cttcattacc
     1501 atggcacctg tattttcaat ccaaagacat aataaaacat cccggcaata aaaataatgt
     1561 tctcaattag cacaacctgg cctttatcat tcgttttgtg cataacccct tcatttccgg
     1621 tattgacgtt gatatcacat ttcgccaaaa aaacgaaatt tttccgtcag ataaccagaa
     1681 tacatcatca ctatggtgaa taagcggaat gttccatagc tgcatcatgc gtgtcttgca
     1741 tcgccttgta caaccttgca tttctcatcg cccatttcga ctgttaaaaa agagagcagc
     1801 agttatttac cgcgtattgt cattaacgtt ttgagaggca ggtattgtga aaggtttaat
     1861 tggtgtactc ggtggaatgg ggccggcagc taccgtcgac ctgtttaata aatttgtaac
     1921 ttttaccgct gcacaatgtg atcaggaaca tattccttta attatttctt cgatcccgga
     1981 tattcctgac cgtaccgatg cattaatgca tcatgggcgt tcaccattac ccgttatgcg
     2041 tgattatatg cataagctgg aagatgccgg ggcagaatgt attgttattc cctgtaatac
     2101 cgcgcattac tggttcaaag aattaaaaga tgcttgtcat attgatatat taagtatcgt
     2161 agaaactacg atcaatgaag ttcgcgcctg tggtaaaacc cgaattggct tgttagccac
     2221 caatgccacg ctatatatgg gtttatacca aaaaggaatt gagtcgctgg gatttacctg
     2281 tgtcagcccc gatgccgacg gtcagaaaaa agtgatggaa agtatttatt ccttgaaagc
     2341 tggcaatata gcgcacgcac aaaagttgat gaatgagcag gctgaaatat tattttcccg
     2401 cggtgcagaa atccttgttc ttggttgtac agaaattccg gttattctgg cgcaagccgt
     2461 taaagagcag ccttcccgct atattgactc aacggcgtca ctcgttcgtg ccggaataaa
     2521 atggtacgaa aatcgtgtcg gtaaacatta tcttttaacc caataatcat ttaaatcgca
     2581 gccagaaagt tattcgcttt taactgaatt atatttataa cggagaacat tatggtttgg
     2641 ctggaaatta tcgtagtact tggtgcaata ttttttggta ttcgccaggg gggaatcggt
     2701 attggtttat gtggcgggct tgggcttgcc attctgactc tgggatttgg tctgcctatg
     2761 gggtcgccgc cagtcgacgt gatcctgatt attatgaccg ttgttgtggc ggcctcagcc
     2821 ttacaggccg caggcggaat ggattatctg gtacgcgtcg ctggtaattt tatgcggcgt
     2881 aacccgaaat atatcaacat aatcgcgccg attatcacct ggtctatgac cattatggcc
     2941 ggtactgggt ttattgtttt ttccacgctg ccggtgattg ctgaagtggc gaaagagtcc
     3001 ggtatccgtc cttcccgcac gcttgccggt tcggttgtcg cttcgcaggt tgcgatttcc
     3061 ggttcgccga tcagcgccgc aatggcggct atgctcacta ttatggaggt aaacggtgtc
     3121 agttttattc aagtgatgtc ggtatgtctt cccacctctt ttgctgccgc gatggtcgcc
     3181 gcttttattg cttcccgaca gggatgtgag ttgcaggatg acgaggtcta ccttgagcgc
     3241 ctgcaaaaag gactggtgca aaaatacgag aataatagca gcatcaagcc aggtgcgacg
     3301 ctctccgtcg gattgttcct gctggcgacg gtcgctattg ttattcttgc tgccttcccc
     3361 caactgcgtc cgggttttga tatcggtaaa ccgatggaaa cacgcgatat cattattatc
     3421 tgtatgctct ctgccgcctg cctgatggtg gtgctgtgta agacggcgac cgatgccatt
     3481 attctcacct ccacattccg tgcggggatg agttcgctgg cggtaattct cggcattgta
     3541 accttaggca ccacttttat tgatgcgcat ttaactgaga ttaaagatat tgctggtgat
     3601 attttgcagg cgtatccgat gttgttggcg ctggttcttt ttggcacttg cgcgctgctc
     3661 tactcgcaag gtgcgaccac gccgttaatt atcccgcttg ccgtcgcgct tgacgtgccc
     3721 acctgggcaa ttctggcgtc ctatgtggcc gtcaccggag tatttgtact gccaacgtat
     3781 ccaacatcac tggctgccat ggaatttgat actaccggca caactcgtgt aggcaaatat
     3841 gtgctgaatc atccatttat gctgcctggg ttggggggaa ttattgctgg agtggcatta
     3901 ggctttgtga ttgcgccaat gattatttag cactatcaaa catcagtatc gatggtttat
     3961 acacaataaa gacagatatg gcggaatata gtctgttgta agcgccaaca aagaataggg
     4021 gaattccgcc atgtctgaac atcttcacaa tatggtatta agtgttgtcg tttaatgatt
     4081 tatttcttgg ttatgttgtg tattagctgc atctatcgct ttttgata
//
LOCUS       DAJTQY010000201         4110 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.203, whole genome shotgun sequence.
ACCESSION   DAJTQY010000201 DAJTQY010000000
VERSION     DAJTQY010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4110
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.203"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(954..1145)
                     /locus_tag="OO349_003985"
     CDS             complement(954..1145)
                     /locus_tag="OO349_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HCR5868866.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESFHDH
                     ADELYRSCLARFGDDGENL"
     gene            1209..1532
                     /locus_tag="OO349_003986"
     CDS             1209..1532
                     /locus_tag="OO349_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HCR5868867.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPT
                     LAPPLLCQLAAIGNNLNQTARKVNSGQWSSGNRVQVVAALMAIGDELRRLRLAVREQG
                     TRDDS"
     gene            1522..1833
                     /locus_tag="OO349_003987"
     CDS             1522..1833
                     /locus_tag="OO349_003987"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5868868.1"
                     /translation="MIVKFHARGKGGGSGPVDYLLGRERNREGATVLQGNPEEVRELI
                     DATPFAKKYTSGVLSFAEKELPPGGREKVMASFERVLMPGLEKNQYSILWVDAPGQGT
                     A"
     gene            1808..2230
                     /locus_tag="OO349_003988"
                     /pseudo
     CDS             1808..2230
                     /locus_tag="OO349_003988"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2211..2741
                     /locus_tag="OO349_003989"
                     /pseudo
     CDS             2211..2741
                     /locus_tag="OO349_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
     gene            2748..2981
                     /locus_tag="OO349_003990"
     CDS             2748..2981
                     /locus_tag="OO349_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HCR5868869.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQVQHLSEQTERLSQLYSENRR"
     gene            complement(3527..3994)
                     /locus_tag="OO349_003991"
     CDS             complement(3527..3994)
                     /locus_tag="OO349_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014526575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5868870.1"
                     /translation="MRIDISHQTRHTPPNMLPREQNCVAMALSACFRQQLNPVVNSLL
                     KERIIHSPKELEHDNAVIRVLQKLQIQEVCNNTLWETAKQQLLQKSDGRYFAINSKHL
                     SFPGPGESHAFCCIKYKNAIGINGNNAETQSTHYQPYPYDKVSIWGPFPHNLT"
BASE COUNT         1001 a          938 c         1194 g          977 t
ORIGIN      
        1 aaaaaaacca ccgctaccaa cggtggtttg tttgccggaa tctagagcta ccaactcttt
       61 cttccgaagg taactggctt ggtggagcac agataccaaa tgctgtcctt ctagtgtagc
      121 cgcagttagg ccaccacttc aagaactctt aatatctcaa tccaccttgt ccagttacca
      181 atggctgctg ccagtggcgt taagtcgtgt cttaccgggt tggactcaag acgatagtta
      241 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
      301 cgaacgacct acaccgaact gagataccaa cagcgtgagc tatgagaaag cgccacgctt
      361 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
      421 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
      481 ctctgacttg agcgtcggtt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
      541 gccatcggcg cggccttctt cctgtccctg gtcttttgct cacatgttct ttccggcgtt
      601 atcccctgat tctgtggata acagcatttc gtttttgagt gagtagatac cgctcgccgc
      661 agtcgaacga ccgagcgtag cgagtcagtg agcgaggaag cggaaaagcg cctggacgtg
      721 cattttctcc ttacgcatct gtgcggtatt tcatacccgg tatggcgcac ttttcataca
      781 atctgcactg atgccgcatg gttaagccag tatacactcc gctatcgcta cgtgactggg
      841 tcatggctgc gccccgacac ccgctaaaac ctgctgatgc gccctgacgg gcttgtctgc
      901 tcccggcatc cgctcacaga caagctgtga gcggatgccg ggagcagcat atgtcagagg
      961 ttttcaccgt catccccgaa acgtgcgagg cagctgcggt aaagctcatc ggcgtggtcg
     1021 tgaaacgatt cacagatatc agcctgttca tccgcgtcca gttcgttgag cttctccagc
     1081 agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgttta
     1141 gtcactgatg cctccgtgta agggggattt ctgttcagga ggtaatgata ccgatgaaac
     1201 gcgagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgct
     1261 gtgagggtaa acagctggcg gtatggatgc ggcgggtctg cctgggggag ccggttgccc
     1321 gttccgggaa actgccgaca ctggcaccgc cgttactgtg tcagctggct gccatcggga
     1381 ataacctgaa tcagacagcg aggaaggtga acagcgggca gtggtcttcc ggtaaccggg
     1441 ttcaggtggt ggccgcactg atggccatcg gggacgagct gcgccggctg cgtctggccg
     1501 tcagggaaca ggggacgcgg gatgatagtt aagtttcatg cccggggaaa aggtggtggc
     1561 agtggtccgg ttgattacct gctgggaagg gagcgtaacc gggaaggggc aacggtgctt
     1621 cagggtaatc cggaagaagt ccgggaactc atcgatgcca cgccatttgc gaagaaatac
     1681 acgtccggtg ttctgtcgtt cgcggagaag gagctgccgc cgggaggacg tgaaaaagtg
     1741 atggcgagct ttgagcgtgt actgatgccc ggtctcgaaa aaaatcagta cagcatcctg
     1801 tgggtggatg caccaggaca agggacggct tgagctgaat tttgtcattc cgaacatgga
     1861 gctacagacc ggaaaacgcc tccagccgta ctacgaccgc gcagacaggc ctagaattga
     1921 tgcctggcag acgctggtta atcactatta cgggctgcat gacccgaacg ccccggagaa
     1981 ccgcaggacg ctgacactcc ctgataatct gccggaaacg aaacaggcga ttgctgaggg
     2041 cgttacacgc gggatagacg cgctttacca tgccggagag ataaaaggcc gtcaggatgt
     2101 gattcaggct ctcacagagg cagggattga cgtggttagg gtgacgcgaa gcagtatcag
     2161 cattgcagac ccgaacggcg ggaagaatat cagactgaaa ggagcgtttt atgagcaatc
     2221 ttttacagac gggtgcggag ttcgagaaaa agctgaaaga gagagccgaa tctacagaga
     2281 aaatgctgaa cgacgagttc aggaagctcg aagaatctgt aaacagggaa ctgacctcaa
     2341 acgaatcttt aatcaggaac gctatcaacg accatacatc agccctgaaa gaacttctgg
     2401 agaggtatca gaaaacgacc gtcgatacga tggacgcaca ctggaaaacc gtactgaaaa
     2461 tgagcgtgaa acgctggctg tggctgatta tcgtctcagt actgatgttt gccacgaccg
     2521 ggagcctgct gtggtaccag ggcatgaaga taaacgcgaa catgaatatt ctccgcgagt
     2581 agaaagagag tctggagaag ctgaacgcga agacatgggg cgtgcgttat cacgaggaca
     2641 gcaacggacg ttttctggtg ttgcccgagg ggatgaaagc cgaaacgaac tggacgatgg
     2701 aaaacgggaa acagaatgcc gtaaggctgg tacaggagta acagagcatg acggagctgg
     2761 aaagacattt gctgaacgca ttagaacaac tgcaacagga ctatatgcag cggctgaacg
     2821 aatgggagag cgccttcgtg gaattgcaga aaatgttttc gcttacgcaa cgggacaacg
     2881 cgatgctgaa cgagcgggtc atgcagttga gtcagcaggt gcagcacttg agcgagcaga
     2941 cagaacgctt gagccagtta tacagcgaga acaggagata agggacgaac ggctgataca
     3001 ggaatgcgaa catgcgttat ccctggagcg tgagcaccag ctggaaatac aggaacggac
     3061 actggatggc ccttcgctgg gatggtaatc agagacaggc taaaatgttg tcagcgcgtt
     3121 tccggtgcat tttttgggat aatggttggt tcgggggcac cgggaacgcg cttcagtcag
     3181 accttgctga aaacatagga acgcgttaaa acgcatacgg gcgcgattct ggtgtgtctt
     3241 ttgttgctgt gttttgcctc tattgccaga tcgcgctcat tcccctgaac ttcttttttc
     3301 ccgctcaggt ccccgtcttc ttcgcttccg aaagcatgaa aaaaatccgt ttcagctgtc
     3361 tctggctggc aattcctgcg tgattcacat ggctgcatag ctatgcatgt ggttaaaatt
     3421 taccgggtgc gatcgcggca gtttttcggg tggtttgttg ccatttttac ctgtctgctg
     3481 ccgtgatcgc gccgaacgcg tttcagcggt gcgtacaatt aagatattac gttaaattgt
     3541 gagggaatgg tccccatata ctgactttat catagggata gggttggtag tgggtcgatt
     3601 gggtttcggc attgtttccg ttaatgccaa ttgcattttt gtatttgatg caacagaagg
     3661 cgtgagattc tcctgggcct ggaaaggata agtgtttaga attgatggca aaatatcttc
     3721 catccgattt ttgaagtagt tgttgtttgg ctgtctccca aagggtgttg ttacaaactt
     3781 cttgaatttg tagtttttgc aggactctta ttactgcgtt atcatgttcc aattctttag
     3841 gggagtgtat tattctctct tttaataagc tgtttaccac agggtttagt tgttgtctaa
     3901 agcacgcaga taatgccatt gctacacaat tttgttcccg aggaagcatg tttggcgggg
     3961 tatgccgtgt ttgatgtgat atgtctattc tcatagaagg tctctggctt atagcatcaa
     4021 caaatacggc tataaaacaa aattttcata gagtgattca agatcttctt gagatccttt
     4081 tttgcggcgc gtaatctgct gcctgcaaac
//
LOCUS       DAJTQY010000202         4029 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.204, whole genome shotgun sequence.
ACCESSION   DAJTQY010000202 DAJTQY010000000
VERSION     DAJTQY010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4029
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.204"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            154..1791
                     /gene="pyrG"
                     /locus_tag="OO349_003992"
     CDS             154..1791
                     /gene="pyrG"
                     /locus_tag="OO349_003992"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HCR5868871.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            1879..3177
                     /gene="eno"
                     /locus_tag="OO349_003993"
     CDS             1879..3177
                     /gene="eno"
                     /locus_tag="OO349_003993"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HCR5868872.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(3237..>4029)
                     /locus_tag="OO349_003994"
     CDS             complement(3237..>4029)
                     /locus_tag="OO349_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HCR5868873.1"
                     /translation="VAAQPTIVPQLQQQVTGLTSSLNSQEKKELTHKLESIFNNTQVQ
                     IAVLIVSTTKDETIEQYATRVFDNWRLGDAKRNDGILIVVAWSDRTVRIQVGYGLEEK
                     VTDALAGDIIRSNMIPAFKQQKLAKGLELAIIALNNQLTSQHQYPANPSESESTSSTD
                     HYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSGVCISAIHLLDLFLFSDKTFSIAVFS
                     FFFTFTIFMVFTCLCVLQKRASGRSYHSDNSGSAGGSSGGASGRW"
BASE COUNT         1070 a          939 c         1013 g         1007 t
ORIGIN      
        1 ccccaaaggg gctattttct cactcctgat ttcaatagtg cgctggcgaa gaggagggat
       61 aatgaaagtt tgtggcacag gtcatgttcg ggtatactgc tttcccgtct tggttattcc
      121 atcgtctttt caacctaact tctcaggttc agcatgacaa cgaactatat ttttgtgacc
      181 ggcggggtcg tatcctctct gggtaaaggc attgccgcag cctccctcgc agccattctt
      241 gaagcccgtg gcctcaatgt gaccatcatg aaactggatc cttacatcaa cgtcgatcca
      301 ggtactatga gcccaatcca acacggggaa gtgttcgtta ctgaagacgg cgctgaaacc
      361 gacttggacc tggggcacta cgagcgtttc attcgtacca aaatgagccg ccgcaacaac
      421 ttcaccacgg gtcgtatcta ctctgacgtt ctgcgtaaag aacgccgcgg tgactacctc
      481 ggcgcaaccg tgcaggttat tccgcacatc actaacgcaa tcaaagagcg cgtgctggaa
      541 ggtggcgaag gtcatgacgt agtactggta gaaatcggcg gtacagtagg tgatatcgaa
      601 tccttgccgt tcctcgaagc tattcgccag atggctgttg aaattggccg tgagcacact
      661 ctgtttatgc acctgacgct ggtgccgtac atggcagcgt ctggtgaagt caaaaccaaa
      721 ccgactcagc actctgtaaa agagctgctc tccatcggta tccagcctga catcctgatt
      781 tgtcgttcag atcgcgctgt accggcgaac gaacgtgcga agattgcatt gttctgtaat
      841 gttccggaaa aagcggttat ttctctgaaa gacgtcgatt ccatctataa aattccgggc
      901 ctgttgaaat ctcaggggct ggacgattat atttgtaaac gattcagctt aaactgcccg
      961 gaagcaaatc tgtccgaatg ggaacaggtt atcttcgaag aagcgaatcc ggtaagtgaa
     1021 gtcaccatcg gtatggtcgg caagtacatt gaactgccgg atgcttataa atcagtgatc
     1081 gaagcactga aacacggtgg gctgaagaat cgtgtcagcg tcaacatcaa actgatcgat
     1141 tcacaagatg ttgaaacgcg cggcgttgaa atccttaaag gtctggacgc aatcctcgta
     1201 cctggcggtt tcggctatcg tggcgtagaa ggcatgatta cgaccgcgcg ttttgcgcgt
     1261 gagaacaata ttccttatct gggcatttgc ctgggtatgc aggtggcgtt aattgattac
     1321 gctcgccatg ttgccaacat ggagaacgcc aactctacag aatttgtgcc agactgtaag
     1381 tacccggttg tggcgctgat taccgagtgg cgcgatgaaa acggcaacgt tgaagttcgt
     1441 agcgagaaga gcgatctcgg cggtaccatg cgtctcggcg cacagcagtg ccagttggtt
     1501 gacgatagcc tggttcgcca gctatacaat gcgccgacaa ttgttgagcg tcatcgtcac
     1561 cgttacgaag tcaacaacat gctgttgaaa cagattgaag atgcaggtct gcgcgttgcg
     1621 ggccgttccg gggatgatca gttggtcgag atcatcgaag ttccgaatca cccgtggttc
     1681 gtggcttgcc agttccatcc ggagtttact tctactccac gtgatggtca cccgctgttt
     1741 gcaggctttg tgaaagccgc cagcgagttc cagaaacgtc aggcgaagta agtaaaaaag
     1801 ttagaacggc aacgcgtacc ttgggtacgc gttgtttgtc tggagtttca gtttaactag
     1861 tgacttgagg aaaacctaat gtccaaaatc gtaaaaatca tcggtcgtga aatcatcgac
     1921 tcccgtggta acccgactgt tgaagccgaa gtacatctgg agggtggttt cgtcggcatg
     1981 gcagctgctc cgtcaggtgc ttctactggt tcccgtgaag ctctggaact gcgcgatggc
     2041 gacaaatccc gtttcctggg taaaggcgta accaaagctg ttgctgcggt aaacggcccg
     2101 atcgctcagg cgctgattgg caaagatgct aaagatcagg ctggcattga caagatcatg
     2161 atcgacctgg acggcaccga aaacaaatcc aaattcggcg cgaacgcaat cctggctgta
     2221 tctctggcta acgccaaagc tgctgcagct gctaaaggta tgccgctgta cgagcacatc
     2281 gctgaactga acggcactcc gggcaaatac tccatgccgg ttccgatgat gaacatcatc
     2341 aacggtggtg agcacgctga caacaacgtt gatatccagg aattcatgat tcagccggtt
     2401 ggcgcgaaaa ctgtgaaaga agccatccgc atgggttctg aagttttcca tcacctggca
     2461 aaagttctga aagcgaaagg catgaacact gctgttggtg acgaaggtgg ctatgcgccg
     2521 aacctgggtt ccaacgctga agctctggct gttatcgctg aagctgttaa agctgctggt
     2581 tatgaactgg gcaaagacat cactctggcg atggactgcg cagcttctga attctacaaa
     2641 gatggtaaat acgttctggc tggcgaaggc aacaaagcgt tcacctctga agaattcact
     2701 cacttcctgg aagaactgac caaacagtac ccgatcgttt ctatcgaaga cggtctggac
     2761 gaatctgact gggacggttt cgcataccag accaaagttc tgggcgacaa aatccagctg
     2821 gttggtgacg acctgttcgt aaccaacacc aagatcctga aagaaggtat cgaaaaaggt
     2881 atcgctaact ccatcctgat caaattcaac cagatcggtt ctctgaccga aactctggct
     2941 gcaatcaaga tggcgaaaga tgctggctac actgcagtta tctctcaccg ttctggtgaa
     3001 actgaagatg ctaccatcgc tgatctggct gttggtactg ctgcaggcca gatcaaaact
     3061 ggttctatga gccgttctga ccgtgttgct aaatacaacc agctgattcg tatcgaagaa
     3121 gctctgggcg aaaaagcacc gtacaacggt cgtaaagaga tcaaaggcca ggcataagtc
     3181 tgactttatc tgatttgata atgccagcct tcgggctggc attttttatg gtggagttac
     3241 cagcggccgg atgcgccgcc acttgagcct ccagcggaac cgctgttgtc tgaatgataa
     3301 cttctaccag atgctctttt ctgaagtaca cataaacagg taaagaccat aaatatggtg
     3361 aaagtaaaaa agaaggaaaa tacagcaatg gaaaaagttt tatcggagaa caggaataaa
     3421 tctaaaagat ggattgctga aatacagact ccacttttac atgcgcgaca aaaattactg
     3481 ccttgatgaa aaaaccagaa agggaagaac atcactgcaa atacccaaaa aatagcaaag
     3541 taataatgat cagtggaaga cgttgattca ctttctgaag gattagccgg atattggtgt
     3601 tgtgaagtga gttggttatt caaagcgatt atagctaact ctaatccctt agctaatttt
     3661 tgttgtttaa atgcgggtat catgttgcta cggatgatat ctccagccag agcatcggtt
     3721 accttttctt ccagcccgta acctacctgg atgcggacag tgcgatccga ccaggcaaca
     3781 acgatcagta tcccatcatt acgtttggca tctcctaaac gccaattgtc aaaaactctt
     3841 gtagcatatt gttcaattgt ctcatctttg gttgtggaaa caattaatac agcaatttgc
     3901 acttgggtat tattgaaaat agattctaac ttgtgcgtca gttctttctt ttcttgtgag
     3961 tttaagctac tcgtaagacc cgtaacttgt tgctgtaatt gtgggacaat agttggttgg
     4021 gctgcaaca
//
LOCUS       DAJTQY010000203         3945 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.205, whole genome shotgun sequence.
ACCESSION   DAJTQY010000203 DAJTQY010000000
VERSION     DAJTQY010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3945
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.205"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            <1..357
                     /locus_tag="OO349_003995"
     CDS             <1..357
                     /locus_tag="OO349_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016042215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR5868874.1"
                     /translation="VFVHTKPAYRSDGIKTAAVRKMRTDSNKAWKGALKRAGISNFRF
                     HDLRHTWASWLVQSGVSLLALKEMGGWETLEMVQRYAHLSAGHLTEHASKIDAIISRN
                     GTNTAQEENVVYLNAR"
     gene            619..981
                     /locus_tag="OO349_003996"
     CDS             619..981
                     /locus_tag="OO349_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HCR5868875.1"
                     /translation="MLKLFVKYTSIGVLNTLIHWVVFGVCIYTAHTSQALANFTGFVV
                     AVSFSFFANARFTFKASTTAMRYMLYVGFMGILSVIVGWAADKCSLPPIVTLITFSAI
                     SLVCGFVYSKFIVFRDAK"
     gene            978..1898
                     /locus_tag="OO349_003997"
     CDS             978..1898
                     /locus_tag="OO349_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCR5868876.1"
                     /translation="MKISLVVPVFNEEEAIPVFYKTVREFQELKPYEVEIVFINDGSK
                     DATESIINALAVSDPLVVPLSFTRNFGKEPALFAGLDHASGDAVIPIDVDLQDPIEVI
                     PHLIEKWQAGAEMVLAKRSDRSTDGRLKRKTAEWFYKLHNKISTPKIEENVGDFRLMS
                     REVVENIKLLPERNLFMKGILSWVGGQTDVVEYVRAERVAGISKFNGWKLWNLALEGI
                     TSFSTFPLRVWTYIGLFVASISFLYGAWMIIDTLVFGNPVRGYPSLLVSILFLGGVQL
                     IGIGVLGEYIGRIYLETKSRPKYILKKGRK"
     gene            1901..2852
                     /locus_tag="OO349_003998"
                     /pseudo
     CDS             1901..2852
                     /locus_tag="OO349_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060427.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gtrI"
     gene            2879..3119
                     /locus_tag="OO349_003999"
                     /pseudo
     CDS             2879..3119
                     /locus_tag="OO349_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060423.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gtrI domain protein"
     gene            3116..3421
                     /locus_tag="OO349_004000"
     CDS             3116..3421
                     /locus_tag="OO349_004000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5868877.1"
                     /translation="MTVNWLSGGDIQITPVFFNADSGKINFTGVRQQTLASWHSKEAF
                     NSAPERQFIAVSIANEPERCKEMTSCLAGIQEQLGKPDEVLNFEGRVILVFNKKLNL"
     gene            complement(3655..>3945)
                     /locus_tag="OO349_004001"
     CDS             complement(3655..>3945)
                     /locus_tag="OO349_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR5868878.1"
                     /translation="SQSVTKMITCGENGHPVIVDRPSPTPEQLALINNEKKSALIAEA
                     TNVIAPLQDAVDLDMATDDETKLLLAWKKYRALLMRVDIKNTEWPKKPESNL"
BASE COUNT         1143 a          772 c          765 g         1265 t
ORIGIN      
        1 gtgtttgtcc acaccaaacc tgcctaccga agcgacggaa taaaaacagc agcggtaagg
       61 aagatgagaa ccgacagcaa caaggcatgg aagggagcgt taaagcgggc aggcattagc
      121 aacttccgct tccatgacct gaggcatacc tgggcaagct ggctggttca gtccggtgtc
      181 tctcttcttg cgcttaaaga gatgggagga tgggaaactc tcgaaatggt tcaaagatac
      241 gcccaccttt cagccgggca tctcaccgag cacgcgagca aaatcgatgc gattataagt
      301 cgcaatggca caaatacggc acaagaggag aacgtagttt acttaaatgc gaggtaactt
      361 attgatttaa atggtgccga taataggagt cgaacctacg accttcgcat tacgaattat
      421 aagaatccgc ttgtaattca aaggattatc ccatcaacac tgcgctcaca cgtcccacca
      481 catcaaaaca tgtaaagcct tgcaagccat tgtgaggcct tatgtgtctc agttttgtct
      541 catcagacat agcaagtatc gatcaattga gacttggatg atagacttca tgcctttcag
      601 agctcattga ttaaataaat gttaaagtta tttgtaaagt acacatctat aggtgtgctg
      661 aacacactta tacactgggt ggtttttggt gtatgtatct ataccgcaca taccagtcag
      721 gctctggcaa acttcacagg tttcgtagtg gctgtgagct ttagcttctt cgcgaatgca
      781 agattcacat tcaaagcatc gactacagcg atgcgctaca tgttatacgt gggatttatg
      841 ggaatattga gtgtgattgt tggttgggca gctgataaat gctcacttcc tccaatagtc
      901 actcttatca ccttctccgc catcagtctg gtgtgcggtt tcgtctattc aaagttcatt
      961 gtctttaggg atgcgaaatg aaaatatctc ttgtcgttcc tgtcttcaat gaagaagaag
     1021 cgatacctgt tttctataaa acggtacgtg aattccaaga gttgaagcca tatgaagtag
     1081 aaattgtatt cataaatgac ggaagtaaag atgccacaga gtcaattatt aacgcgctgg
     1141 ctgtttcaga cccgctagtt gttccgctgt catttacacg caactttggt aaagaaccag
     1201 ccttatttgc agggttagac catgcaagcg gcgatgctgt aattcctatt gatgtcgacc
     1261 tgcaagaccc aattgaggtt atccctcatc ttattgaaaa gtggcaggca ggtgctgaga
     1321 tggtgcttgc taaacgttca gaccgctcaa ctgatggacg actgaaacgt aagacagctg
     1381 agtggttcta taaattacac aacaaaataa gcaccccaaa gatcgaggaa aatgtcggag
     1441 attttcgact catgtctcgt gaggttgtgg agaacattaa actgttgcct gagcgcaatc
     1501 ttttcatgaa aggcatactg agctgggtgg gtggtcagac ggatgtcgtt gaatatgtac
     1561 gcgcagagcg tgttgctggc atctcaaaat ttaatggctg gaaattatgg aatctggcac
     1621 tggaaggtat cacaagtttt tcaacctttc ctcttcgcgt atggacttat ataggcttgt
     1681 ttgttgcaag catttcattt ttatatggtg catggatgat tatagacacc cttgtctttg
     1741 gtaacccagt acgcgggtat ccctccctgc ttgtatcaat acttttcttg ggtggagtgc
     1801 aactgatcgg gattggtgtt ctcggtgaat atataggtag aatttactta gaaacaaaat
     1861 caagacctaa gtatattttg aagaaaggta gaaaataacc atgagcatat gtattaaaca
     1921 atcagcatta aaaatattat tggctttatc agcactccta ataacttggt tgactaccag
     1981 atactttcca gttgaaccgg atgttgctaa cagcccaatt gtatggaggc atattttaga
     2041 gaatggtata tcatcaatcc acgactggaa accaaccgtg gataattggt atttcaccgt
     2101 ttacccaatt aactttttat tttatatgct gttaggtgat gatggcttag tagcattaag
     2161 attatcaact gcagttttct ctattgctat agtaatagca gcgatgttaa cgctacgtaa
     2221 ggcattcggg tttacccctg ctttattttc tattatactt ctatctctaa taccatattt
     2281 ttcatacact tatggttttg tatcacatcc tttttctcat aattcaacaa acgctttcgg
     2341 ctttttatgc ttgcttatat ctgttttcaa catacagtat aaaaatatat ttatcactct
     2401 cctcttaagt ttgacagcat tattttccag cgtatccgat ccgtggttta cagctgcatt
     2461 ttttattcca ctattaatat cttacttttt attctctgta tgggataaaa aattatttaa
     2521 gcatactgca ttaatactat ttgcatgttt aatctctctt tccaatgtat tgcaaaatct
     2581 acttaacata cctccccacc aatttgaaat tgtatcactc aatgatatga ttttaaatgc
     2641 aaaatggtgc attctgttaa taggaaaaag tcttaattta ttagttgtcg acaataacgc
     2701 aacatcttac gcctcatttg tcatttggtt cattgccatt attacatctg cgtggtttgt
     2761 tttatcggac aataaaaaaa acacataccg tatttatatt gtgttatttt cattattatc
     2821 tatcgcaggt attgtctcat cattcatact gagctataaa tcccctgatt atattagcat
     2881 gcgtttcttt atgaatgtta cctgtttcgc actgatattg tgctgtatcg gtacatcaac
     2941 aaaagcaaag atattatttt atctaatcgc atttttattt tcgatcagtt caattaaatc
     3001 ttacacaaat aatgcttcac cattacacga tcaggagaag attgttaaat catacattga
     3061 ttttttaaaa aaaaataatc tccattatgg gtacggctct ttttgggatt tgtcaatgac
     3121 tgtaaattgg ttatctggtg gggacataca gataactcct gttttcttta atgctgattc
     3181 ggggaaaatt aactttactg gcgtgagaca acagacactg gcttcttggc atagcaagga
     3241 ggcgttcaat tctgctcctg agcgacagtt tatagcagtt tccatagcta acgaacctga
     3301 acgatgtaaa gaaatgacat cctgccttgc aggtattcaa gaacaattag gtaagcctga
     3361 cgaagttttg aactttgaag gaagagtaat attagtattt aacaaaaaac ttaacctata
     3421 gtaaattgcc ccctaccata taaaccacac taacgaatga ataacccgtc tttttcgacg
     3481 ggttatatcc ctatcaggaa taaaaatgga gtggttgccg tatacaacaa accctcattt
     3541 agagctatat tctagagtat atgagatact ataaagttga aggagctatc attagaaaaa
     3601 ccatatgctg ttataattag atgtctcgct tttcatccaa taacgaattt tctcttatag
     3661 attgctctct ggttttttcg gccactctgt attttttata tcaacacgca tcaatagtgc
     3721 gcgatatttt ttccatgcca gtaacagttt cgtttcatca tctgttgcca tatctaaatc
     3781 aaccgcatcc tgaagcggcg ctattacatt cgttgcctct gctatcagtg cagatttctt
     3841 ttcattattt attaaggcta attgttctgg tgttggagaa ggacggtcaa caatgacagg
     3901 gtgaccattc tcaccacagg taatcatttt tgttacactc tgcga
//
LOCUS       DAJTQY010000204         3333 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.207, whole genome shotgun sequence.
ACCESSION   DAJTQY010000204 DAJTQY010000000
VERSION     DAJTQY010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3333
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.207"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            <1..465
                     /gene="pgrR"
                     /locus_tag="OO349_004002"
     CDS             <1..465
                     /gene="pgrR"
                     /locus_tag="OO349_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HCR5868879.1"
                     /translation="RLGGEMDKDMIAIRIGPDIPMAIVGSPDYFSRRSAPTSVSQLID
                     HQAINLYLPTSGTANRWRLIRGGREVRVRMEGQLLLNTIDLIIDAAIDGHGLAYLPYD
                     QVERAIKEKKLIRVLDKFTPDLPGYHLYYPHRRHAGSAFSLFIDRLKYKGAV"
     gene            802..2415
                     /gene="mppA"
                     /locus_tag="OO349_004003"
     CDS             802..2415
                     /gene="mppA"
                     /locus_tag="OO349_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HCR5868880.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFIPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSMHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(2466..>3333)
                     /gene="ynaI"
                     /locus_tag="OO349_004004"
     CDS             complement(2466..>3333)
                     /gene="ynaI"
                     /locus_tag="OO349_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HCR5868881.1"
                     /translation="AVGSISNYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLIN
                     QFEKQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVG
                     MAGKDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLY
                     VPNSLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQT
                     LLVYFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTL
                     YMDNITPPEQGR"
BASE COUNT          938 a          725 c          780 g          890 t
ORIGIN      
        1 cgtctgggtg gggagatgga taaagatatg atcgccattc gaatcgggcc agatatacca
       61 atggctattg ttggctcacc ggattatttt tctcgtcgaa gtgctccaac gtcagtgtca
      121 caattaatag atcatcaggc aattaatttg tatcttccca catcgggtac agcaaatcgc
      181 tggagattaa tacgcggtgg acgtgaagtt cgtgttcgca tggaaggtca gcttttactg
      241 aatacgatag acctgatcat tgatgctgca attgatgggc atggattggc gtatctacct
      301 tatgatcagg ttgagcgggc tattaaagaa aaaaaactga tacgtgtttt ggataaattc
      361 actccagatt tacccggtta tcacctgtac tatccacacc gtcgacatgc tggctcggca
      421 ttctcattat ttatagatag gctgaagtat aaaggtgctg tttagcacta cttgctgata
      481 cattaattta attcttctct taacgtattc tcagttcctt tcaacgtttt ggttattttt
      541 tattcttcgt acaatggcga cagatgctga ttatgatacc gaaaactggt ttgaacgtgc
      601 gaagcccgaa cgtagtgttc ggtgttctat gtgctttgcc acattttggc gtctattatt
      661 tacactaaat ctgatttgat atattgatac ttaaaacatt tgatgcttcc tttgtcactt
      721 ttttgatgga agttgtttgc atttctttaa ggcgaaacaa ataattacgc atcaatttta
      781 atgtcggtta gagggaaact tatgaagcac tctgtttcag ttacgtgttg tgcgctgttg
      841 gtcagcagca tttctctttc gtatgctgca gaagttccga gcggcacagt actggcagag
      901 aagcaggagc tggtgcgcca cattaaagat gagcctgcgt cgctggatcc cgctaaagcc
      961 gtgggcctgc cagagattca ggtcattcgc gatctgtttg aaggtctggt gaatcagaac
     1021 gaaaaagggg agattgtccc cggcgtcgcg actcaatgga aaagtaatga caaccgtatc
     1081 tggactttta ccctgcgcga taacgcaaaa tgggcggatg gcacaccggt aacggcgcaa
     1141 gattttgtct acagctggca acgtctggtg gacccaaaaa cattgtcgcc atttgcatgg
     1201 tttgccgcgc tggcgggaat caacaacgca caggcgatta ttgatggtaa agctacgcct
     1261 gatcagcttg gcgtcaccgc agttgatgcc catactttga aaattcagct tgataaaccg
     1321 ttgccgtggt ttgtgaattt aaccgctaac tttgccttct tcccggtgca aaaagccaac
     1381 gtagaaagcg gtaaagagtg gacgaaaccc ggaaatctga tcggcaatgg cgcttatgtt
     1441 cttaaagagc gcgtagtcaa tgaaaaactg gtcgtggtac cgaataccca ttattgggat
     1501 aacgccaaaa cggtgctgca aaaagtgacc ttcctgccaa ttaatcagga atccgcagcc
     1561 actaagcgtt acctcgcggg ggatattgat atcaccgaat ccttcccgaa aaatatgtat
     1621 cagaagctgt tgaaggatat tccggggcag gtttatacgc caccgcagct cgggacctat
     1681 tattatgcct ttaacacgca aaaagggccg acggcagatc agcgcgttcg cctggcatta
     1741 agtatgacga tagatcgccg cctgatgacc gaaaaagtgt tggggacggg cgaaaagcca
     1801 gcgtggcatt ttataccaga tgttaccgcc ggatttacgc cggaaccttc gccgtttgaa
     1861 caaatgagtc aggaagaact gaatgcgcag gcaaaaactt tgttgagcgc agctggttat
     1921 ggtccgcaaa aaccgctgaa gctgacgctt ttgtataaca cttcagaaaa ccatcaaaaa
     1981 attgcgattg ctgtagcatc gatgtggaaa aagaaccttg gcgtagatgt taaattgcaa
     2041 aatcaggaat ggaaaaccta tatcgatagc cgtaacaccg gcaattttga tgttatccgc
     2101 gcctcatggg tgggggatta taatgaaccc tccactttcc tgacattatt aacgtcaatg
     2161 cattcaggaa atatttcacg ctttaacaat ccggcatacg acaaagttct ggcccaggca
     2221 tcgacggaaa ataccgttaa agcgcgtaat gccgattaca acgcggcaga aaaaatcctc
     2281 atggagcaag caccgattgc accaatttat caatatacca atggacgatt aatcaaaccg
     2341 tggctgaaag gttatcccat taataaccct gaagatgtag cgtacagtcg gactatgtat
     2401 attgtgaagc attgatgtga tgggaactgg cgttaccctt gtggataacg ccagtgatgt
     2461 ctgatttagc gcccctgttc cggcggtgta atgttatcca tatacagcgt ctggctgggg
     2521 aaggcaaagt ccgcgccgtg tgactgtaca atatcgataa tcttcaaata aacgtcttgc
     2581 tgtgcagcaa gccactcagc ccataccgtg gttttggtaa agcaataaac cataatattc
     2641 aatgacgagt cagcaaactg gttgaaataa accagtaagg tttgtcgctg gtcgatggcc
     2701 gggtgatttt tcagcatctc acgtacggct tcgacaataa cgcccacttt tgccgcatcc
     2761 tcataacgta aaccaatggt cgtggtaatg cggcggttgg tcattcgtcc tgggttttct
     2821 acgctgatcg acgaaaacag cgagttcggt acgtacaatg gacgattatc aaaggtcgta
     2881 attttggtaa ttcgccagcc aatttccgct actgtacctt cgatatttct gtccggtgaa
     2941 cggatccagt cgccaatact gaaaggacgg tcgaaataga gcatgatccc ggaaaagaaa
     3001 ttactcagaa tatctttacc ggccataccg acagccagac caccaatacc accaaaggtc
     3061 agcaagccag aaaggctcat gccgaaatgt tcgccataaa gcagaacaag cactacaata
     3121 atggtgattt tgataatgcg cgacataatc cgcgcactgg tgatatcgcg acctttttta
     3181 atctgctgtt tttcaaactg attaatcagc agaaatagct taatcgtcag aataaccgca
     3241 atcagggacg tacagataaa atcgataacg cctggggtga taaatttgag tttatagttt
     3301 tctataacat aattactgat gctaccaaca gca
//
LOCUS       DAJTQY010000205         3279 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.208, whole genome shotgun sequence.
ACCESSION   DAJTQY010000205 DAJTQY010000000
VERSION     DAJTQY010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3279
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.208"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            <1..1513
                     /gene="btsS"
                     /locus_tag="OO349_004005"
     CDS             <1..1513
                     /gene="btsS"
                     /locus_tag="OO349_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HCR5868882.1"
                     /translation="WFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            1510..2229
                     /gene="yehT"
                     /locus_tag="OO349_004006"
     CDS             1510..2229
                     /gene="yehT"
                     /locus_tag="OO349_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HCR5868883.1"
                     /translation="MIKVLIVDDEPLARENLRIFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            2276..2746
                     /locus_tag="OO349_004007"
     CDS             2276..2746
                     /locus_tag="OO349_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HCR5868884.1"
                     /translation="MISNDILRSVRYILKANNNDLVRILALDNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(2787..3248)
                     /locus_tag="OO349_004008"
     CDS             complement(2787..3248)
                     /locus_tag="OO349_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HCR5868885.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKSTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
BASE COUNT          824 a          760 c          854 g          841 t
ORIGIN      
        1 ctggtttggg ttgcacattg acgattctat tgccaatacc cgtgcgatag gcgcggtaat
       61 gggcggctta ctcggcggtc cggtcgtcgg tgggctggtt ggtctgaccg gcggcttaca
      121 tcgatattcg atggggggca tgaccgcgct aagttgcatg atctcaacca tcgttgaagg
      181 attgctcggc ggcctggtac acagcatcct gatccgtcgc gggcgcactg ataaagtctt
      241 taaccccatt accgccggtg ccgtcacgtt cgtcgctgaa atggtgcaaa tgctgatcat
      301 ccttgcgatc gcccgacctt atgaagatgc ggtgcgtctg gtgagtaata ttgctgcgcc
      361 aatgatggtc accaataccg tcggcgcggc gctgtttatg cgtatattgc tcgataaacg
      421 cgcgatgttt gaaaaataca cttcggcttt ttctgccact gcgctgaaag tggcagcctc
      481 gacggaaggc attttgcgac aggggtttaa cgaagtgaac agcatgaaag tggctcaggt
      541 actgtatcag gagctggata ttggtgcagt cgcgattacc gatcgagaga aattgctggc
      601 ctttaccgga attggtgacg accaccattt acccggcaaa ccgatttctt cgacttacac
      661 cttaaaagcg attgaaaccg gtgaagtggt ctacgctgat ggcaacgaag taccttatcg
      721 ttgctctttg catccgcaat gcaaactggg gtcgacgctg gtaattccgt tgcgtggcga
      781 aaatcagcgc gtgatgggca ccatcaaatt gtatgaagcc aaaaaccggt tattcagttc
      841 aataaaccgc acgctgggcg aggggattgc gcaactgctt tcggcgcaga ttcttgccgg
      901 gcaatatgag cggcaaaaag cgatgctcac ccagtcagaa atcaaactgc ttcacgccca
      961 ggtgaatcct cattttttgt ttaatgcgct taacaccatt aaagcggtga tccgccgcga
     1021 cagcgaacag gccagccagc tggtgcagta tctttccact tttttccgca aaaacttaaa
     1081 gcggccttcg gagtttgtta ctctcgccga cgaaattgaa catgtgaacg cttatctgca
     1141 aattgaaaag gcgcgcttcc agtcgcggtt gcaggtcaac attgctattc cgcaagaatt
     1201 atcccagcag caattgcccg cgtttaccct gcaaccgata gtggaaaacg ccattaaaca
     1261 tgggacatca caactgctgg atacagggcg agtggcaatc agcgcccgac gtgaggggca
     1321 acatttgatg ctggagatcg aagacaatgc cggtttgtat caaccggtaa ccaatgccag
     1381 tgggctgggg atgaatctgg tggataagcg tttacgtgaa cggtttggcg atgactatgg
     1441 aataagcgtc gcctgtgagc ctgatagtta cacccgaata acgttacgac taccatggag
     1501 ggacgaggca tgattaaagt cttaattgtc gatgatgaac cgttagcacg ggagaacctg
     1561 cgtatatttt tgcaggagca gagcgatatt gaaatcgttg gagagtgttc aaacgccgtg
     1621 gaagggatcg gcgcggtgca taaactgcgc ccggatgtgc tgtttctcga tatccagatg
     1681 ccgcgcatca gtggtctgga aatggtgggg atgcttgacc cggaacatcg cccgtatatt
     1741 gtttttctca ctgcgtttga cgaatacgct attaaagcct ttgaagaaca tgcctttgat
     1801 tatctgctga agccaattga tgaagcgcga ctggagaaaa cgctggcgcg attgcgtcag
     1861 gagcgcagca agcaggatgt ttcgctgtta ccggaaaatc aacaggcgct gaaatttatc
     1921 ccttgtacgg ggcatagtcg gatttatttg ctgcaaatga aagatgtggc atttgtcagc
     1981 agtcggatga gcggtgtcta cgttaccagc cacgaaggga aagagggctt taccgaattg
     2041 acattacgta ccttggaaag tcgtacacca ctactgcgct gccatcgtca gtatctggtt
     2101 aacctcgcgc atttacagga gattcgtctg gaagataacg gccaggccga gttgattttg
     2161 cgtaatggct taaccgtgcc ggtcagccgc cgttatctga aaagcttaaa agaggcgatt
     2221 ggcctgtaaa agactgctaa aatggctttt tgcctcatca acacctgaag gcctcatgat
     2281 aagtaacgat attctgcgca gcgtgcgcta cattttgaaa gccaataata atgacctggt
     2341 gcgtattctg gcgctggata atgtcgaagc caccgcggaa cagatcgccg tctggctacg
     2401 taaagaagac gaagagggtt ttcagcgttg tccggacatt gttttgtcgt cattcctcaa
     2461 tggcctgatt tatgaaaaac gcggcaagga tgagtctgct ccggcactgg agccggaacg
     2521 tcgcattaat aacaacatcg tgctgaaaaa attacgcatc gcgttttcgc tgaaaaccga
     2581 tgacattctg gctatcctca ccgaacagca gttccgcgtt tcgatgccgg aaattacagc
     2641 gatgatgcgc gcaccggatc ataaaaactt ccgcgaatgc ggcgatcaat ttttacgtta
     2701 ttttctgcgt ggactggcag cgcgtcagca tgtgaagaaa agctaagacg ggtatggcgg
     2761 ccatgcgaaa catggccgcc gacagattat ttcacttctt taaaaccagc ggctttcatc
     2821 accagttcca tttgcgccat agtgatacct tttttggcat cttcagcaga aacgttgatt
     2881 cctgaaatac cctgcagggc tttaaaatcc actttttcca tatcgatagt cacgttttcc
     2941 tgcgcgtagg tatcggtata ggttgatttt tcttcaacac ccgcgatgtt tttgtatttg
     3001 gcgcttaacg gctcaagtgt cttggcagca tcttctttgg tggttgcacc aatggaggca
     3061 aattgaattt tggtttcaga agattgctta agcaccttgt cacctttgta gacataggta
     3121 atggcaattt cagtgccgtt cagattggcg ctgaatttct tcgattcttc tttgtcaccg
     3181 cagccagcaa gagagaaaac cagaacagat gcaacaacga gggaaaacag cttattgaaa
     3241 gccttcatgt aaaactccat tttatttaat caagaaact
//
LOCUS       DAJTQY010000206         3178 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.210, whole genome shotgun sequence.
ACCESSION   DAJTQY010000206 DAJTQY010000000
VERSION     DAJTQY010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.210"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            417..1526
                     /gene="fepE"
                     /locus_tag="OO349_004009"
     CDS             417..1526
                     /gene="fepE"
                     /locus_tag="OO349_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HCR5868886.1"
                     /translation="MKISKVSDEHFSNYNMLPNGNEEIDLFGLISVLWAAKIKIVGVI
                     LFFACIGFLVSFVLPQKWTSSAVVTPAESVQWSELNKELSKLHVLGVGFDINRDSAFA
                     LFIKKFQSVNSLNEYMRSSPYVMEIIKKNNISALELHRAIVELSKNMKSIDDNASKKN
                     DKSSLYVSWTLSFTAPTSKEAHDVLSGYINYVSSLVVRDLMEDIRNKLEVKTNVEKEI
                     LALDEIKIRNQLNADIRRLNYSLEVANAAGIKKPVYSNGQIMKDDPDFPVALGSDGIA
                     TKLNIKKSIKDVSELSGELRNRQYVVNQLVVAKVGDVDFMPFQYQLSPTLPVRKDGPS
                     NAIIVILSSVIGGMLACGYILLRHAMEARKMSVIE"
     gene            complement(2551..2745)
                     /locus_tag="OO349_004010"
                     /pseudo
     CDS             complement(2551..2745)
                     /locus_tag="OO349_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
BASE COUNT          800 a          658 c          775 g          945 t
ORIGIN      
        1 aaaaaaaaca tcatcttata ttaggggtgc tgctagcggc gcggtgtgtt tttttacagg
       61 acgccctggg ggcgctgcta ggggtgtctg ttcagatatg tagcaatgag tcagggattc
      121 acgaactgaa aatgtccgaa aatgtgtctc atgggcactg tatgcgccca aaccgatcgt
      181 cattcacttt catgcatagc tatgcagtga gctgaaagcg atcctgacgc atttttccgg
      241 tttaccccgg ggaaaacatc tctttttgcg gtgtttgcgg cagaatcgcg ttcagcgcgt
      301 tttagcggtg cgcgcaataa aatgttatgt taaatgtctg ctgaatggtt tttaggtttt
      361 ttcctaacat atgatagaat aaaaaaacca tatgttaata tattgtgttt ttttcaatga
      421 aaatctcaaa ggtctctgac gaacattttt ctaattacaa catgttgccg aatggtaatg
      481 aagaaattga cctttttggc ttgattagcg ttttatgggc agcaaaaata aaaattgttg
      541 gggtgattct attttttgct tgtattggct ttcttgtttc ttttgttttg ccacaaaaat
      601 ggactagttc tgctgtggtt actcctgctg agtctgttca atggagtgaa ttaaataagg
      661 agctttccaa gcttcatgtt ctgggagtgg gttttgatat caatcgtgat agcgcatttg
      721 ctctgtttat taagaaattt cagtccgtta attcgttgaa tgagtatatg agatcatctc
      781 cttatgttat ggagattatt aaaaagaata acattagcgc tctcgaactg catcgtgcaa
      841 ttgttgagtt gagtaaaaat atgaagtcga ttgatgataa tgccagtaag aaaaacgaca
      901 agtcatcatt gtatgtatca tggactctga gttttactgc tccaacaagt aaagaagctc
      961 acgatgtgtt gtctgggtat attaattatg tttcttccct tgttgtaagg gatttgatgg
     1021 aagatataag aaataaacta gaagttaaaa ctaatgttga aaaagaaatt cttgcactgg
     1081 atgagataaa aattagaaac cagctgaatg cagatattcg acgcctcaat tattcactgg
     1141 aggttgctaa tgcggctgga ataaaaaaac ctgtatacag caatggtcag attatgaagg
     1201 atgacccaga ttttcctgtg gctctcggtt ctgatggtat agcaactaaa ttgaacatca
     1261 aaaaatcaat caaggatgtt tcggaattga gtggggagtt gcgaaatcgt caatatgttg
     1321 tgaatcaatt ggttgtggcg aaagtggggg atgttgattt tatgccattt cagtatcaat
     1381 tgagcccaac gttaccggtc agaaaggatg ggccaagtaa tgccataatt gtaattcttt
     1441 cttctgtcat tggagggatg cttgcatgtg gctatatact tttacgtcat gccatggagg
     1501 ccagaaagat gagtgtgatt gagtaaaagt tgtgctgatg catcatttta taagcaactt
     1561 ttataaaaag ttcaaaggat cttcttgaga tcattccctt acagccgtag tcttctactc
     1621 tagaaaacga aaaaaccacc tggggaggtg gtttttcgaa ggttcagtaa gttggggaac
     1681 ttctgaaccg tggtaacagt cttgtgcgag acatgtcacc aaatctgtcc ttctagtgta
     1741 gcctcagtaa ggctgccact tcaagaactc tcaatacatc tctcgcacat cctgtttacc
     1801 agtggccgct gccaatggcg ttaagtcgtg tcttaccggg ttggactcaa gacgatagtt
     1861 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     1921 gcgaactgcc tacccggaac tgagtgtcag gcgtggaatg agacgaacgc ggccataaca
     1981 gcggaatgac accggtaaac cgaaaggcag gaacaggaga gcgcacgagg gagccaccag
     2041 ggggaaacgc ctggtatctt taagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     2101 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccaccg gcgcggcctt
     2161 ttttcggggc ctctcttttg ctcacatgtt ttttctggtg ttatcccctg attctgtgga
     2221 taaccgtata tccgtctttg cgtgagcaga caccgctcgc cgcagccgaa cgaccgagcg
     2281 cagcgagtca gtgagcgagg aagcggaaga gcgccggaag ctgcattttc tccttacgta
     2341 tctgtgcagc atttcacacc tcatatgtcg ctgttctctc accatctgct ctgccgcctc
     2401 ataattaagc cagtatacac tccgttatcg ctacgtgact gggtcatggc tgcgccccga
     2461 cacccgctaa aaccccctga cgcgccctga cgggcttgtc tgctgacgaa aacgcccacg
     2521 ggaaagccgt gaccgcctcc gtcagcttcg ttatgttcct gtttcacttt cggcctcaaa
     2581 gcgaatctgg atgctgttct ggagttcttc tgcgagttcg tgcagtcgtt tgcacatggc
     2641 cgcctgttcg tcggcatcca gagcatccag tttttcaagc agtgtcaggc tctggctttt
     2701 gatacatcgc gccatattca gcgcagtttg ttgttatttg ttcaccttcc gttgttctcc
     2761 gtcagttccg ggtgtcgggg gggggaccct gagcaggtgg taattgtaag acgttgcggg
     2821 cgatggtatt acaattgcac atcctgtcct gtttctttag gacgtatcat ggcacagcat
     2881 atcaactgat aaataattcc tgtgcagtgc agccgctgtt gcataaaatt tcgctctgcg
     2941 tcgagtgttt cttaatcaat aattccgctg atactccgga aaaacgcgag tctgttctca
     3001 tgcctgaaat gcccacaccc cacgcaaaaa caagtttttg ctgatttttc tttataaata
     3061 gagagttatg acaaattagt tcttcttgct ctctttgtga tatttaaaaa agcggtgtcg
     3121 gcgcggtgtt gtagctgcgc caacaccgct ttttaggggt ggtactgact attttcat
//
LOCUS       DAJTQY010000207         3099 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.211, whole genome shotgun sequence.
ACCESSION   DAJTQY010000207 DAJTQY010000000
VERSION     DAJTQY010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3099
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.211"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            8..2911
                     /locus_tag="OO349_004011"
     rRNA            8..2911
                     /locus_tag="OO349_004011"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            3007..>3099
                     /gene="rrf"
                     /locus_tag="OO349_004012"
     rRNA            3007..>3099
                     /gene="rrf"
                     /locus_tag="OO349_004012"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          815 a          682 c          969 g          633 t
ORIGIN      
        1 gttgtgaggt taagcgacta agcgtacacg gtggatgccc tggcagtcag aggcgatgaa
       61 ggacgtgcta atctgcgata agcgtcggta aggtgatatg aaccgttata accggcgatt
      121 tccgaatggg gaaacccagt gtgattcgtc acactatcat taactgaatc cataggttaa
      181 tgaggcgaac cgggggaact gaaacatcta agtaccccga ggaaaagaaa tcaaccgaga
      241 ttcccccagt agcggcgagc gaacggggag cagcccagag cctgaatcag tgtgtgtgtt
      301 agtggaagcg tctggaaagg cgtgcgatac agggtgacag ccccgtacac aaaaatgcac
      361 atactgtgag ctcgatgagt agggcgggac acgtggtatc ctgtctgaat atggggggac
      421 catcctccaa ggctaaatac tcctgactga ccgatagtga accagtaccg tgagggaaag
      481 gcgaaaagaa ccccggcgag gggagtgaaa aagaacctga aaccgtgtac gtacaagcag
      541 tgggagcacg cttaggcgtg tgactgcgta ccttttgtat aatgggtcag cgacttatat
      601 tctgtagcaa ggttaaccga ataggggagc cgaagggaaa ccgagtctta actgggcgtt
      661 aagttgcagg gtatagaccc gaaacccggt gatctagcca tgggcaggtt gaaggttggg
      721 taacactaac tggaggaccg aaccgactaa tgttgaaaaa ttagcggatg acttgtggct
      781 gggggtgaaa ggccaatcaa accgggagat agctggttct ccccgaaagc tatttaggta
      841 gcgcctcgtg aattcatctc cgggggtaga gcactgtttc ggcaaggggg tcatcccgac
      901 ttaccaaccc gatgcaaact gcgaataccg gagaatgtta ttacgggaga cacacggcgg
      961 gtgctaacgt ccgtcgtgaa gagggaaaca acccagaccg ccagctaagg tcccaaagtc
     1021 atggttaagt gggaaacgat gtgggaaggc ccagacagcc aggatgttgg cttagaagca
     1081 gccatcattt aaagaaagcg taatagctca ctggtcgagt cggcctgcgc ggaagatgta
     1141 acggggctaa accatgcacc gaagctgcgg cagcgacgct tatgcgttgt tgggtagggg
     1201 agcgttctgt aagcctgtga aggtgtgctg tgaggtatgc tggaggtatc agaagtgcga
     1261 atgctgacat aagtaacgat aaagcgggtg aaaagcccgc tcgccggaag accaagggtt
     1321 cctgtccaac gttaatcggg gcagggtgag tcgaccccta aggcgaggcc gaaaggcgta
     1381 gtcgatggga aacaggttaa tattcctgta cttggtgtta ctgcgaaggg gggacggaga
     1441 aggctatgtt ggccgggcga cggttgtccc ggtttaagcg tgtaggctgg ttttccaggc
     1501 aaatccggaa aatcaaggcc gaggcgtgat gacgaggcac tacggtgctg aagcaacaaa
     1561 tgccctgctt ccaggaaaag cctctaagca tcaggtaaca tcaaatcgta ccccaaaccg
     1621 acacaggtgg tcaggtagag aataccaagg cgcttgagag aactcgggtg aaggaactag
     1681 gcaaaatggt gccgtaactt cgggagaagg cacgctgata cgtaggtgaa gcgacttgct
     1741 cgtggagctg aaatcagtcg aagataccag ctggctgcaa ctgtttatta aaaacacagc
     1801 actgtgcaaa cacgaaagtg gacgtatacg gtgtgacgcc tgcccggtgc cggaaggtta
     1861 attgatgggg ttagcgcaag cgaagctctt gatcgaagcc ccggtaaacg gcggccgtaa
     1921 ctataacggt cctaaggtag cgaaattcct tgtcgggtaa gttccgacct gcacgaatgg
     1981 cgtaatgatg gccaggctgt ctccacccga gactcagtga aattgaactc gctgtgaaga
     2041 tgcagtgtac ccgcggcaag acggaaagac cccgtgaacc tttactatag cttgacactg
     2101 aacattgagc cttgatgtgt aggataggtg ggaggctttg aagtgtggac gccagtctgc
     2161 atggagccga ccttgaaata ccacccttta atgtttgatg ttctaacgtg gacccgtgat
     2221 ccgggttgcg gacagtgtct ggtgggtagt ttgactgggg cggtctcctc ctaaagagta
     2281 acggaggagc acgaaggttg gctaatcctg gtcggacatc aggaggttag tgcaatggca
     2341 taagccagct tgactgcgag cgtgacggcg cgagcaggtg cgaaagcagg tcatagtgat
     2401 ccggtggttc tgaatggaag ggccatcgct caacggataa aaggtactcc ggggataaca
     2461 ggctgatacc gcccaagagt tcatatcgac ggcggtgttt ggcacctcga tgtcggctca
     2521 tcacatcctg gggctgaagt aggtcccaag ggtatggctg ttcgccattt aaagtggtac
     2581 gcgagctggg tttagaacgt cgtgagacag ttcggtccct atctgccgtg ggcgctggag
     2641 aactgagggg ggctgctcct agtacgagag gaccggagtg gacgcatcac tggtgttcgg
     2701 gttgtcatgc caatggcact gcccggtagc taaatgcgga agagataagt gctgaaagca
     2761 tctaagcacg aaacttgccc cgagatgagt tctccctgac cctttaaggg tcctgaagga
     2821 acgttgaaga cgacgacgtt gataggccgg gtgtgtaagc gcagcgatgc gttgagctaa
     2881 ccggtactaa tgaaccgtga ggcttaacct tacaacgccg aagatgtttt ggcggattga
     2941 gagaagattt tcagcctgat acagattaaa tcagaacgca gaagcggtct gataaaacag
     3001 aattagcctg gcggccgtag cgcggtggtc ccacctgacc ccatgccgaa ctcagaagtg
     3061 aaacgccgta gcgccgatgg tagtgtgggg tctccccat
//
LOCUS       DAJTQY010000208         3054 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.212, whole genome shotgun sequence.
ACCESSION   DAJTQY010000208 DAJTQY010000000
VERSION     DAJTQY010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3054
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.212"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            532..1698
                     /gene="nhaA"
                     /locus_tag="OO349_004013"
     CDS             532..1698
                     /gene="nhaA"
                     /locus_tag="OO349_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HCR5868887.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLVLKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMVVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            1764..2663
                     /gene="nhaR"
                     /locus_tag="OO349_004014"
     CDS             1764..2663
                     /gene="nhaR"
                     /locus_tag="OO349_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HCR5868888.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAVR"
     gene            complement(2701..>3054)
                     /locus_tag="OO349_004015"
     CDS             complement(2701..>3054)
                     /locus_tag="OO349_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HCR5868889.1"
                     /translation="YQFHHIRFDCIQGEQGELVFEPSDVEYYFEPVTIQESGTSILKN
                     ELENSEDGAGQIGFQLSNDGTNEIQYGKSNYYSFQHPHEGSNQIPLFIRPRTYGNNVS
                     SGQIMSRVKIVVMYN"
BASE COUNT          718 a          677 c          750 g          909 t
ORIGIN      
        1 catataggag gcctcgggtt gatggtaaaa tatcactcgg ggcttttctc tatctgccgt
       61 tcagctaatg cctgagacag acagcctcaa gcacccgccg ctattatatc gctctcttta
      121 acccattttg ttttatcgat tctaatcctg aagacgcctc gcatttttgt ggcgtaattt
      181 tttaatgatt taattattta actttaattt atctcttcat cgcaattatt gacgacaagc
      241 tggattattt ttgaaatatt ggcctaacaa gcatcgccga ctgacaacaa attaattatt
      301 acttttccta attaatccct caggaatcct caccttaagc tatgattatc tgggcttagg
      361 gtcactcgtg agcgcttaca gccgtcaaaa acgcatctca ccgctgatgg cgcaaattct
      421 tcaatggctc gtaaaaaacg aattattcct acactataat ctgattttaa cgatgattcg
      481 tgcggggtaa aatagtaaaa acgatctatt cacctgaaag agaaataaaa agtgaaacat
      541 ctgcatcgat tctttagcag tgatgcctcg ggaggcatta ttcttatcat tgccgctatc
      601 ctggcgatga ttatggccaa cagcggcgca accagtggat ggtatcacga ctttctggag
      661 acgccggttc agctccgggt tggttcactc gaaatcaaca aaaacatgct gttatggata
      721 aatgacgcgc tgatggcggt atttttcctg ttagtcggtc tggaagttaa acgtgaactg
      781 atgcaaggat cgctagccag cttacgccag gccgcatttc cagttatcgc cgctattggt
      841 gggatgattg tgccggcatt actctatctg gcttttaact atgccgatcc gattacccgc
      901 gaagggtggg cgatcccggc ggctactgac attgcttttg cacttggtgt actggcgctg
      961 ttgggaagtc gtgttccgtt agtgctgaag atctttttga tggctctggc tattatcgac
     1021 gatcttgggg ccatcattat catcgcattg ttctacacta atgacttatc gatggcctct
     1081 cttggcgtcg cggctgtagc aattgcggta ctcgcggtat tgaatctgtg tggtgtacgc
     1141 cgcacgggcg tctatattct tgttggcgtg gtgttgtgga ctgcggtgtt gaaatcgggg
     1201 gttcacgcaa ctctggcggg ggtaattgtc ggcttcttta ttcctttgaa agagaagcat
     1261 gggcgttctc cagcgaagcg actggagcat gtgttgcacc cgtgggtggc gtatctgatt
     1321 ttgccgctgt ttgcatttgc taatgctggc gtttcactgc aaggcgtcac gctggatggc
     1381 ttgacctcca ttctgccatt ggggatcatc gctggcttgc tgattggcaa accgctgggg
     1441 attagtctgt tctgctggtt ggcgctgcgt ttgaaactgg cgcatctgcc tgagggaacg
     1501 acttatcagc aaattatggt ggtggggatc ctgtgcggta tcggttttac tatgtctatc
     1561 tttattgcca gcctggcctt tggtagcgta gatccagaac tgattaactg ggcgaaactc
     1621 ggtatcctgg tcggttctat ctcttcggcg gtaattggat acagctggtt acgcgttcgt
     1681 ttgcgtccat cagtttgaca ggacggttta ccggggagcc ataaacggct cccttttcat
     1741 tgttatcagg gagagaaatg agcatgtctc atatcaatta caaccacttg tattacttct
     1801 ggcatgtcta taaagaaggt tccgtggttg gcgcagcgga ggcgctttat ttaactccac
     1861 aaaccattac cggacagatt cgagcgctgg aagagcgcct gcaaggcaaa ttatttaaac
     1921 gcaagggacg tggtctcgaa cccagcgagc tgggagaact ggtctatcgc tatgccgata
     1981 aaatgttcac cttaagccag gaaatgctgg atattgtgaa ctatcgcaaa gaatccaatt
     2041 tattgtttga cgttggcgtg gctgatgcac tttccaaacg cctggtcagt agcgtactta
     2101 acgccgcagt ggtagaaggc gagcccattc atcttcgctg cttcgaatcc acccacgaaa
     2161 tgctgctgga gcaattaagt cagcataaac tggatatgat catttctgac tgtccgatag
     2221 actctacgca gcaggaaggc ctgttctccg tgagaattgg cgaatgtggc gtgagtttct
     2281 ggtgtacaaa tccaccacca gaaaaaccgt tcccggcttg tctggaagaa cggcgacttt
     2341 tgattcctgg gcgacgttca atgttagggc gcaaattgct taactggttt aactcccagg
     2401 gattaaacgt agaaatcctc ggcgagtttg atgatgccgc tttgatgaaa gcttttggtg
     2461 cgatgcacaa tgcaatcttc gttgccccaa cgctttatgc atatgacttt tatgccgata
     2521 aaactgtcgt agaaattggt cgcgtcgaga atgtgatgga agagtaccat gctatttttg
     2581 ctgagcggat gattcagcac ccggcggtac agcgaatctg caatacggat tattctgcgc
     2641 tttttagtcc agcggtgcgt taatcggcag ctcccccaaa gttaaggtgg gggagataga
     2701 ttagttgtac attaccacga ttttgactcg gctcattatt tgcccgcttg agacattgtt
     2761 tccatatgta cgcgggcgaa taaatagagg aatctgatta cttccttcat ggggatgctg
     2821 aaaagagtag taattgcttt taccatactg gatttcattc gtaccatcat tggagagttg
     2881 aaagcctatt tgacccgcac cgtcctcact gttttctaac tcatttttta atatgctggt
     2941 tcctgactcc tgtatcgtca ctggttcaaa gtaatattca acgtcactgg gttcaaaaac
     3001 taattcacct tgctcgccct ggatgcagtc aaatcttata tgatgaaact gata
//
LOCUS       DAJTQY010000209         2969 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.213, whole genome shotgun sequence.
ACCESSION   DAJTQY010000209 DAJTQY010000000
VERSION     DAJTQY010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2969
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.213"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            <1..570
                     /locus_tag="OO349_004016"
     CDS             <1..570
                     /locus_tag="OO349_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCR5868890.1"
                     /translation="AELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPG
                     EQTATSILKQRTKNLAPLPHAHQQQNPPQEETVVSIAVDPESPAQYLQRQKPQREEMP
                     VYTRWVKTQKCMTCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCHNELHAGVKD
                     FEEKHGSQLLLLIRFLMHARNSGVLKWKA"
     gene            571..936
                     /locus_tag="OO349_004017"
     CDS             571..936
                     /locus_tag="OO349_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR5868891.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVR
                     QQRLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVC
                     GQLVPGGRLGIKITELGCA"
     gene            933..1616
                     /locus_tag="OO349_004018"
     CDS             933..1616
                     /locus_tag="OO349_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HCR5868892.1"
                     /translation="MNNQYLQFVREQLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGTSIPLVSPVEFSTSSWRRAVLSLEEHYK
                     AWLLWCYSGSICWEYQIAITQWAWNEFNAQSGTRKIAGKTQERLKKLIWLAAQAVKAE
                     LFGGEGYEYQELALLAGVTTKNWSKTFTRHWVAMKHIFHRLDSEALLFVMRTRSKKRR
                     HFQSKVLQK"
     gene            1815..1890
                     /locus_tag="OO349_004019"
     tRNA            1815..1890
                     /locus_tag="OO349_004019"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1848..1850,aa:Ile,seq:cat)
     gene            1892..1968
                     /locus_tag="OO349_004020"
     tRNA            1892..1968
                     /locus_tag="OO349_004020"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1926..1928,aa:Lys,seq:ttt)
     gene            1971..2046
                     /locus_tag="OO349_004021"
     tRNA            1971..2046
                     /locus_tag="OO349_004021"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2004..2006,aa:Thr,seq:tgt)
     gene            2050..2124
                     /locus_tag="OO349_004022"
     tRNA            2050..2124
                     /locus_tag="OO349_004022"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2083..2085,aa:Gly,seq:tcc)
     gene            2417..2632
                     /locus_tag="OO349_004023"
     CDS             2417..2632
                     /locus_tag="OO349_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HCR5868893.1"
                     /translation="MKSMDKISTGIAYGTSAGSAGYWFLQWLDQVSPSQWAAIGVLGS
                     LVLGFLTYLTNLYFKIREDKRKAARGE"
     gene            2731..>2969
                     /locus_tag="OO349_004024"
     CDS             2731..>2969
                     /locus_tag="OO349_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5868894.1"
                     /translation="MNSQTTKDIPCFRSYLPDALRLRFEDKLTIRAIAQRLGLSHSTI
                     HTLFQRFLASGIAWPLPDSVSFAQLDAILYANRKK"
BASE COUNT          772 a          671 c          779 g          747 t
ORIGIN      
        1 gctgaattat cctggtgggc ggtccgcaat caggtggcgg acgcgctacc ggaagcggta
       61 ttacgtcgtt cgctggggtt gcgtgcggaa aaaatccgct caatgtaccg tgaaagcgac
      121 atcgtgccgg gagagcagac cgccaccagc atactgaagc agcgcacaaa aaatcttgcg
      181 ccgctgcctc acgcccacca gcaacagaat ccaccacagg aagagacggt ggtcagcatt
      241 gccgttgatc cggaatctcc ggctcagtat ctccagcgcc agaaaccaca acgggaagag
      301 atgcctgtat acacgcgctg ggtaaaaacg cagaaatgca tgacgtgtgg caatcaggca
      361 gatgatccgc accacatcat tggtcatgga ctgggaggga tgggaacaaa ggctgacgat
      421 ttgtttgtta ttccgctgtg tcgtaaatgc cataacgaac tacacgccgg ggtaaaagat
      481 tttgaagaaa aacacggtag tcagttgttg ttgctgattc gttttttaat gcacgcgaga
      541 aattcgggtg ttctgaagtg gaaagcataa atgaccgaac gcatagaatt tgttttgcct
      601 tacccgccaa cggtgaacac ctactggcga cgtcgtggca gcacatattt tgtatcaaaa
      661 gccggtgagc gttatcgccg tgatgtggcg ctaattgttc gccagcagcg actgaaatta
      721 aacctgtccg gaaggctggc gataaagatt attgcagagc caccggataa gcgccgtcgt
      781 gacctggaca atatcctgaa ggcaccgctg gatgcgctga cgcatgctgg actacttata
      841 gacgacgagc agtttgatga aatcaatatt gtgtgcggtc agctcgttcc tggtgggcgg
      901 ctggggataa aaatcacaga actggggtgc gcatgaataa ccagtattta cagtttgtgc
      961 gtgagcagct cattatcgcc accgctgatt tgagtggggc aacaaaaggt cagcttgaag
     1021 cctggcaaga gaatgccatg ttcgatacag ggcgttacag gcgtaaaaaa atccggtacc
     1081 gcgatgaagt gactggaaaa atgataacgc gggataatcc accaatcccg ggaaagcaat
     1141 cgctggcgaa ggggacgtca attcctctgg tcagtccggt tgagttttcg acatcatcgt
     1201 ggcggcgggc tgttctgtct cttgaagagc actataaagc ctggttgttg tggtgttaca
     1261 gcgggagtat ttgttgggaa tatcagatcg cgataacaca gtgggcgtgg aatgaattta
     1321 atgctcaatc cggtaccaga aaaattgcag ggaaaacgca ggaacgcctg aaaaaattaa
     1381 tctggctggc ggcgcaggca gtaaaagcag aactttttgg tggggaaggt tatgaatacc
     1441 aggagctggc attactggcg ggagtgacaa ctaaaaactg gtccaaaaca tttactcgtc
     1501 actgggttgc aatgaaacac atttttcacc gactggatag tgaggcttta ttgtttgtaa
     1561 tgagaacacg ttcaaaaaaa agacggcatt ttcaaagcaa agtgttgcaa aagtagattg
     1621 aaaggcatat attcatgcaa atctgatact ttgccgattt tgtacgtgat ggcaaaagca
     1681 aacaaaaccc gcccacaagc gggttttttg tgccacttat ctcggataga catggtgaat
     1741 gcgctggtgg aggagataag ggtgattttt gaatgcttgc aacattgatt tcgtaacgtt
     1801 attatcctgc gcccggccct ttagctcagt ggtgagagcg agcgactcat aatcgccagg
     1861 tcgctggttc aaatccagca agggccacca accgccacta gctcatcagg aaagagcgtc
     1921 aaccctttaa gttgagtgtg cgaggttcga gtccccggtg gcggtccagt gccgacttgg
     1981 ctcagtaggt agagcaactg acttgtaatc agtaggtcac cagttcgatt ccggtagtcg
     2041 gcaccatatg cgggcatcgt ataatggcta ttacctcagc cttccaagct gatgatgcgg
     2101 gttcgattcc cgctgcccgc tccagtcaga gtctttcagt ctgcgatgat gggaaatccc
     2161 ggagtgactg aaagacgttt aagttatgaa tgatcgcttt tttttgcaaa attgctgtgc
     2221 agaaatacta accttcgggc aggcgatcat tcataagcac tctgctttta ttccgattaa
     2281 ctgtgagtgg tttgttggat agagtgcttt ccttactgta tatatcgttt cgcccgcttt
     2341 tgcggttttt tcttttcaaa tccctttcat ttctcagtgt aaaactacgc catccgttat
     2401 ttgcggaggt gaggctatga aatccatgga caaaatttca acgggcattg cctacggcac
     2461 ctccgcaggc agtgctggct actggttttt acagtggctt gatcaggtca gtccgtcaca
     2521 gtgggctgcg attggtgtac tggggagtct ggttctgggc ttcctgactt atctgacaaa
     2581 tctgtacttc aaaatcagag aagacaagcg taaggctgcg agaggtgaat aatgtcgcca
     2641 tcattacggt aagcgcccca tcagcgacgt cttgtgaaaa ttgtcctgtc tggcaacaat
     2701 cgcgcccatc tatattgatg gacacgaacg atgaattccc agacaacaaa agatattccc
     2761 tgcttccgtt cttatttgcc tgatgccctg cgtttaagat ttgaagataa actgaccatc
     2821 cgggccatcg ctcagcgtct gggtctcagt cattccacaa tacatacgct ttttcagcga
     2881 tttcttgcat ccggtatcgc atggccattg cccgattcag tttcattcgc tcaacttgac
     2941 gccatccttt atgccaacag aaagaagga
//
LOCUS       DAJTQY010000210         2951 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.214, whole genome shotgun sequence.
ACCESSION   DAJTQY010000210 DAJTQY010000000
VERSION     DAJTQY010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.214"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            141..2009
                     /gene="kup"
                     /locus_tag="OO349_004025"
     CDS             141..2009
                     /gene="kup"
                     /locus_tag="OO349_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HCR5868895.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGGEVLYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            2176..2595
                     /gene="rbsD"
                     /locus_tag="OO349_004026"
     CDS             2176..2595
                     /gene="rbsD"
                     /locus_tag="OO349_004026"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HCR5868896.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2603..>2951
                     /locus_tag="OO349_004027"
     CDS             2603..>2951
                     /locus_tag="OO349_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR5868897.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWK"
BASE COUNT          658 a          702 c          779 g          812 t
ORIGIN      
        1 aagggggggc tttttctttg agccaggtta atgtggccgc atttaggagt acgattttgc
       61 cgttaatcgt gcatactgtg cgcttttttg tgggccaagg gactaagcac acatttcata
      121 tttcaacgaa agactagtct atgagcactg ataataagca atcattgccc gcgattaccc
      181 tcgcggcgat tggagttgtc tacggcgata ttggtaccag cccgttatat acacttcgtg
      241 aatgtttgtc cggccagttt ggttttggcg ttgaacgcga tgccgtgttt ggctttttat
      301 cgctgatctt ctggctgcta atctttgtgg tttccattaa atatctcacc ttcgtgatgc
      361 gggcagataa cgccggtgag ggggggatcc tgacgttgat gtcgcttgcc gggcgtaata
      421 cgtcggcgcg aaccacatca atgctggtga ttatggggct aatcggcggc agctttttct
      481 atggtgaagt cgtcataaca cccgctattt cggtgatgtc agccattgaa ggtctggaaa
      541 tcgtcgcccc gcagctggat acctggatag ttcccctctc aattatcgtt ctcactttat
      601 tatttatgat tcaaaagcat ggcacggcta tggtcggtaa gctgtttgcg ccgatcatgc
      661 tgacctggtt tttgattctg gcaggactgg ggttacgtag cattattgct aacccggaag
      721 tgctgcatgc gctgaatccg atgtgggcgg tgcatttctt ccttgaatac aaaacggttt
      781 cttttattgc attaggggca gtggtgctgt cgattacggg gggcgaggtg ctgtatgctg
      841 atatggggca ctttggtaag ttccctattc gcctggcgtg gttcaccgtc gtattacctt
      901 ccttgaccct taattacttc ggtcagggag cgttgttgtt aaagaacccg gaagcgatta
      961 agaacccgtt cttcctgttg gcaccggact gggcgctgat cccactgctg atcatcgccg
     1021 cactggcgac ggtaattgcc tcgcaggcgg ttatctctgg cgtcttctca ttgacgcgtc
     1081 aggcggtacg tctgggatat ttgtcgccga tgcgcattat tcacacctcc gaaatggagt
     1141 cagggcaaat ctatattccg tttgtgaact ggatgctcta tgtcgcggtc gtgattgtga
     1201 ttgtcagctt tgagcactcc agcaacctgg cggcggcgta cgggattgcg gtgaccggaa
     1261 ccatggtact gacgtctatt ctctcgacta ccgtggcacg tcagaactgg cactggaata
     1321 agtattttgt tgcgctgatc ctgattgctt tcctttgtgt cgatattcca ttgttcaccg
     1381 ctaacctcga taaactgctc tccggcggct ggttgccatt gagcctcggt actgtgatgt
     1441 ttatcgtgat gaccacctgg aagagcgagc gtttccgctt gctgcggcgg atgcatgaac
     1501 atggtaactc tctggaagcg atgattgctt cgctggagaa atcaccgcct gttcgcgtgc
     1561 ccgggaccgc ggtgtatatg tcgcgtgcaa tcaacgtcat tccctttgcg ctgatgcata
     1621 accttaaaca taacaaggta ttgcatgagc gggtgattct gttaactctg cgcaccgaag
     1681 acgcgccata tgtccataac gtccgtcggg tacagattga acaactgtcg cccactttct
     1741 ggcgcgtggt ggcaagttat ggttggcgag aaacgccaaa cgtggaagaa gttttccacc
     1801 gctgcggtct ggaaggatta agttgccgga tgatggaaac ctccttcttt atgtcgcatg
     1861 agtcgttgat cctcggcaaa cgcccgtggt atttgcgtct gcgcggcaag ctgtacttgc
     1921 tgctgcaacg taatgcgctg cgtgcaccag atcaatttga aatcccgcca aacagggtta
     1981 tcgaactggg tactcaggtc gaaatctaac gccagacgcc tcctttcttc ataagggggc
     2041 gtttttgttt tcatggttaa tcaccatgta aaacgtttcg aggttgatca catttccgta
     2101 acgtcacgat ggttttccca actcagtcag gattaagctg cggatcagcg aaacgtttcg
     2161 ctgatggaga aaaaaatgaa aaaaggcacc gttcttaatt ctgatatttc atcggtgatc
     2221 tcccgtctgg gacataccga tacgctggtg gtgtgtgatg ctggtttacc catccccaaa
     2281 agtacaacgc gtatcgatat ggcattaacc caaggtgtac cttcttttat gcaggtgctg
     2341 ggcgtcgtca caaatgaaat gcaggtcgag gctgccatta tcgcggaaga gatcaaacaa
     2401 cataatccgc aactccacga aacgttgctc actcaccttg agcagctgca aaaacaccag
     2461 ggaaatacca ttgaaattcg ttacaccacg catgaacaat tcaaacaaca aaccgcagaa
     2521 agtcaggcgg taattcgcag cggagaatgt tctccgtatg cgaatatcat tctctgtgct
     2581 ggcgtgacgt tctgaggccg tcatggaagc attacttcag cttaaaggca tcgataaagc
     2641 cttcccgggc gtaaaagccc tctcgggcgc agcgttaaat gtctatccgg gccgtgtgat
     2701 ggcgctggtg ggcgaaaacg gcgcgggtaa atccaccatg atgaaagtgc ttactggtat
     2761 ctatactcgc gatgccggca cgcttttatg gctggggaaa gaaacgacat ttaccgggcc
     2821 gaaatcttcc caggaagccg ggattgggat tatccatcag gaactgaacc tgatcccgca
     2881 gttgaccatt gccgaaaaca ttttcctcgg tcgtgagttt gttaatcgct ttggcaaaat
     2941 tgactggaaa a
//
LOCUS       DAJTQY010000211         2927 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.215, whole genome shotgun sequence.
ACCESSION   DAJTQY010000211 DAJTQY010000000
VERSION     DAJTQY010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2927
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.215"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            <1..782
                     /gene="lsrF"
                     /locus_tag="OO349_004028"
     CDS             <1..782
                     /gene="lsrF"
                     /locus_tag="OO349_004028"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HCR5868898.1"
                     /translation="DWGMQSRLSWIFNPKTGKTVMLAFDHGYFQGPTTGLERIDINIA
                     PLFEHADVLMCTRGILRSVVPPATNKPVVLRASGANSILAELSNEAVALSMDDAVRLN
                     SCAVAAQVYIGSEYEHQSIKNIIQLVDAGMKVGMPTMAVTGVGKDMVRDQRYFSLATR
                     IAAEMGAQIIKTYYVEKGFERIVAGCPVPIVIAGGKKLPERETLEMCWQAIDQGASGV
                     DMGRNIFQSDHPVAMMKAVQAVVHHNETADRAYELYLSEKQ"
     gene            806..1096
                     /gene="lsrG"
                     /locus_tag="OO349_004029"
     CDS             806..1096
                     /gene="lsrG"
                     /locus_tag="OO349_004029"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HCR5868899.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDTVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            1135..1893
                     /gene="tam"
                     /locus_tag="OO349_004030"
     CDS             1135..1893
                     /gene="tam"
                     /locus_tag="OO349_004030"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HCR5868900.1"
                     /translation="MSDWNPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     TRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(1897..2811)
                     /locus_tag="OO349_004031"
     CDS             complement(1897..2811)
                     /locus_tag="OO349_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HCR5868901.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTGDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEEPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
BASE COUNT          771 a          678 c          781 g          697 t
ORIGIN      
        1 tggattgggg aatgcagtca cgcttatcgt ggatatttaa tccgaaaacg ggtaaaactg
       61 tgatgctggc ctttgaccat ggttattttc aggggccgac taccggactt gaacgcattg
      121 atataaatat cgccccgctg tttgaacatg ccgatgtatt aatgtgtacg cgcggcattt
      181 tgcgcagcgt agttcccccg gcgaccaata agccggtggt actgcgggcg tcaggtgcga
      241 actctattct ggcggaatta agtaatgaag ccgtggcgtt atcgatggat gatgccgtgc
      301 gcctgaacag ttgcgcggtg gcggcgcagg tttatatcgg cagcgaatat gaacatcagt
      361 cgatcaaaaa tattattcag ctggtcgatg ccggaatgaa agtgggaatg ccgaccatgg
      421 ccgtgactgg cgtgggcaaa gatatggtgc gcgatcagcg ttatttctcg ctcgcgactc
      481 gaatcgccgc tgaaatgggg gcgcaaatta tcaaaaccta ttatgtcgaa aaaggttttg
      541 aacggattgt tgccggatgt ccggtaccca ttgttattgc tggcggtaaa aaattaccgg
      601 agcgcgagac gctggaaatg tgctggcagg ctatcgatca gggcgcttct ggtgtggata
      661 tggggcgtaa tattttccag tctgaccatc cggtggcgat gatgaaagcc gtacaggcgg
      721 tggttcacca taacgaaacg gctgatcggg catatgaact ctatctgagt gaaaaacagt
      781 aactgcggat ttaaggagaa gaattatgca cgtcacactg gttgaaatta acgttcatga
      841 agacaaggtt gacgagttta tcgaagtttt tcgccagaac cacctgggct ctgtacagga
      901 agaaggtaat ttgcgcttcg atgtcttaca ggacccggaa gtgaattcgc gcttttatat
      961 ttacgaagcc tataaagatg aagacacagt ggcattccat aaaaccacgc ctcactacaa
     1021 aacctgtgtc gcgaaactgg aatctttaat gaccgggccg cgtaaaaaac gtctgttcaa
     1081 tgggttgatg ccgtgaggct acaattgggg taacgcgctg acgggagtaa aaaaatgtct
     1141 gactggaacc cctctttata tctgcacttt tccgctgaac gatcgcgtcc ggcggtggag
     1201 ctgcttgcca gagtgccgct ggagaatgtc gaatatgttg ccgatcttgg ctgtggccca
     1261 ggtaacagca ccgccctgct gcatcaacgt tggcctgcgg ccaggataac aggcatcgac
     1321 tcgtctccgg cgatgattgc tgaagctcgc agtgctttgc cagactgcca gttcgtggaa
     1381 gcagacatcc gcaactggca accggaacag gcactcgatc tgatttttgc taatgcctca
     1441 ctgcaatggc tgcccgacca ctacgaattg tttcctcact tggtttcttt acttaatccg
     1501 cagggcgtgc tggcagtaca gatgccagat aactggctgg agccgaccca tgtgctcatg
     1561 cgcgaagttg cttgggaaca aaactacccg gatcgcgggc gtgagccgtt ggctggcgtt
     1621 catgcttact acgatatttt gagcgaagcc ggatgtgagg tcgatatctg gcgaaccact
     1681 tactatcacc agatgccgtc gcaccaggcg attatcgatt gggtgaccgc cactggatta
     1741 cgtccgtggt tacaggatct gaccgagagc gaacagcagc tttttcttac gcgctaccat
     1801 cagatgctgg aagagcagta tccactgcaa gagaacggac agatactgct ggcatttccg
     1861 cgtctgttta ttgttgcccg ccgtacggag taattatcac gtcagctggt aatgacgatc
     1921 gggaagaatt tttgctggaa tttctgcttc atcgttcatc tgtaacaggt caatttcaat
     1981 agcgttgcag atggcatcca gcggtaaatc attgttttca gtaccgaacg gttcttccag
     2041 ttcttccgcc agacaatcca gcgaaataaa agtgtaggaa atcagcacag agataaaagg
     2101 cgtcatgtaa tgcaggtcca cgaccagcgc gaacggcagc atgatacaaa acagataaac
     2161 ggtacgatgc aaaatcaggg tgtaggcaaa gggaattggc gtgtaggcaa tgcgctcaca
     2221 gcctgccaga accgctgaaa tatcattaag ccgatcgttg aggctaataa acaggatatc
     2281 tgaaagctgt ccattgcggc gctgaaccgc caaccattct cccattatta acaagatacg
     2341 gttagctgga gagttcgaag ccagtacacg ctgaagatct ccagtcttga gataatgagc
     2401 cagcacttcc gcctgtggct gtttgcgtaa tgtcatgcgt aaacagtggg cgaaggcgat
     2461 ttgcagcctg gcaaactccc ttacacttgc cgaatccggc aatgtcgttt ttacctctcg
     2521 cagtaacgac cgtgaggcaa tcatcaactg cccccaaagt tttcgcgctt caacgtaacg
     2581 ggcgtacccg gcattattac gaaaaccaag aaaaatggcg atggcgacac cgagaatgct
     2641 gaacggtgcg agggtgaatt tgatgcccag atgcgtgtac cagggcagca tgaatataac
     2701 agcaatagaa aagagaaaat tgagtagtaa gcgcgaggat atcttggata atactgagcc
     2761 gtgccagaca aaaatacggc gcagccagtg ttgttgtgga cgaacaatca tggttatctt
     2821 caggcgtgga aaagtcgccc tattaaacgt gattacgatc acattctcaa gacgctactt
     2881 acaaattacc tagaaccacg gcagcctgac ggctgcaaat tgaaaac
//
LOCUS       DAJTQY010000212         2917 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.216, whole genome shotgun sequence.
ACCESSION   DAJTQY010000212 DAJTQY010000000
VERSION     DAJTQY010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2917
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.216"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(<1..1255)
                     /locus_tag="OO349_004032"
     CDS             complement(<1..1255)
                     /locus_tag="OO349_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR5868902.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPGIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPEL
                     LETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDNMLTDIPYLP
                     ENLRNGYFDINQISHIPESILNLRNECSIDISDNPLSSHALQSLQRLTSSPDYHGPQI
                     YFSMSDGQQNTLHRPLADAVTAWFPENKQSDVSQIWHAFEHEEHANTFSAFLDRLSDT
                     VSARNTSGFREQVAAWLEKLSASAELRQQSFAVAADATESCEDRVALTWNNLRKTLLV
                     HQASEGLFDNDTGALLSLGREMFRLE"
     gene            complement(1856..2104)
                     /locus_tag="OO349_004033"
     CDS             complement(1856..2104)
                     /locus_tag="OO349_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HCR5868903.1"
                     /translation="MRFEICIAKEKMTKMPTGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(2459..2806)
                     /locus_tag="OO349_004034"
                     /pseudo
     CDS             complement(2459..2806)
                     /locus_tag="OO349_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
BASE COUNT          738 a          569 c          736 g          874 t
ORIGIN      
        1 tttcgaggcg gaacatttcc ctgcccaggg agagcagagc gccggtatca ttatcgaaaa
       61 ggccttctga tgcctgatgg accaggaggg ttttccggag attgttccat gtgagcgcga
      121 cacggtcctc acagctttca gtggcatcag cagcaacagc gaaagactgc tgtcgaagct
      181 ccgcagaggc actgagtttt tccagccatg cagcgacctg ttcacggaat ccggaggtat
      241 tgcgtgcaga gacggtatcg gaaaggcggt caaggaacgc ggaaaaggtg ttggcgtgct
      301 cttcatgttc aaaagcatgc catatctgtg atacatcaga ttgtttgttt tccgggaacc
      361 atgctgtcac ggcatcagcc agggggcgat ggagtgtatt ctgttgtccg tcactcatgg
      421 agaagtaaat ctgcgggccg tggtagtccg gcgaagatgt taatctttgc agggattgca
      481 gagcatggga tgacaatggg ttatcactaa tatctattga acattcattc ctcagattaa
      541 gaatgctttc cgggatatga cttatctgat ttatgtcaaa ataaccgttc cttaaatttt
      601 ccggcaggta tggaatatcg gttaacatat tgtcgattgc ccatatggag tgaatgttgt
      661 gaggtagagg ggggagttca ctaagaagat tccctattgc gctaatctct tgtaaagaaa
      721 atggtaaagg tggtaaaact gccagcccat tacatgatac atctaatgtt tccagtagtt
      781 ctggtaaaac aggaagagaa cataattgat tatttgagac atgaagctcc ttcaaagata
      841 tggggagtgt gggtagtgtg attagttgat tgtgggacgc attcaataat ttaagtcctt
      901 gaggcaaagc aggcagttca ataagtctgt tatagctgac attaagttgt gtaagggacg
      961 caggtaatgg ggagattaag cttaaattat ttttacttat attaattgat ttaattcccg
     1021 gggggatttc aggtaatgtt gtcaggccta attcagacaa gtccaggttc gtctcttggt
     1081 tttgtagaca tgatactagt cgctgaaaag cgatgtctcg ttgttcttct tgtatgcggt
     1141 tatttttcca ttcagtccaa tgggttaggt aattttcata tgcgcggcta gtgtcgattg
     1201 aataagtgga gaacgaattt gaaattaatc tgtgattgtt atttgtcggg agcataaata
     1261 tcagggcgta ttctttatgt tgagaggtgt tggattcttt tttatctgct ttgcaatgtt
     1321 attggttccc ttcagcagta gacaggaact tctggaggag gcggatatat agtttgtgta
     1381 agcatattaa ctccatatgt tatatattga agaacttctg ctttacacta tcctaaactg
     1441 gatcggttta atgtaaaaaa acgatacttt ttgggggggg gggcaatttt tccttaaggt
     1501 atgaaaaaag gagctgaagc tatataatag ctattaatgc cactggtatc agtgaatcat
     1561 gtatataaaa aactgtgtta aggaggctgt ccattagcgg ttttttattc ttctggaacc
     1621 gcggcatctt tgcgtatctt tttgtcttct caccgtctgg tcggtgtttt gctgagactg
     1681 ctaatttcct gtctttgcta gtgctgtact ggtactgccc aatcatgatt ggcgggggcg
     1741 ggcggagttg agaccgcaga cacgtcgtat gcaagaacgt gctgcggctg gctggtgaac
     1801 ttccgatagt gcgagtattg aatgatttcc agccgttact gattttacgt gctaattagt
     1861 gaacaaacca ctcgtcagca gactcccagg tatctttcag agtttcctga acaaaagttt
     1921 tagctgaatc tttatcggcg gtgcgcgtaa cagaaaggcc atcgttgctg gtggctttta
     1981 cgatcacctc tacatcgtca taacgtttac tgatgcgtcg ggttaattct tcctttaacg
     2041 catccacagc accggttggc attttagtca ttttctcttt ggctatgcag atttcaaaac
     2101 gcataaaaat ccctctgcac tgtgttttta tacagtatta tttttaactg tatggataaa
     2161 cagtgtcaag agtgagggaa agtgattttt tggggcatgc atggggcaaa aatatttgtt
     2221 gtggggcatt ttggggcatg attgggacac tttgttacat atgaactttg ccgattttca
     2281 tatgacatgg caatccaacg ctttgattat tctaaagaaa acacatgctc ttgggcgttc
     2341 tttagtgatt tttaaaaatc ccgtatcacg cagttaaagt ggcgggcata ctcttcaagg
     2401 ctggtgatcc ccaaccgcac ccatttagga tgtgaccatt gcggtagacc gatgtaaatc
     2461 ataacgcgtg aaggatctct tccacgctac gcacacgggc gatgcgcggg tagatatgat
     2521 taatgctgtt attgtgttgc tcggcgctgg cggcactaca ggcgtcttcg gcaatcacca
     2581 gattaaagcc aagttcccag gcattgcggg cggtggattc aacaccgata ttggtcgaga
     2641 tcccacataa cactattgta tcgatacctc ggcggcgcag ttgcaactcc agatccgtac
     2701 cgtaaaacgc accccactga cgtttgatga tttcgatatc actgtcagtt gcacctaatg
     2761 cagctggatg ttgccaccag ttttcgggca acactttagc gggggagtgc ccctattaag
     2821 ttgtcaagca tgttatgacc cctgcggggt ataaaaagtc ccgtcgcgca tcatggcgaa
     2881 cagaacgtcg cagcgtcgtc tcgccagggc gataagc
//
LOCUS       DAJTQY010000213         2807 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.217, whole genome shotgun sequence.
ACCESSION   DAJTQY010000213 DAJTQY010000000
VERSION     DAJTQY010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.217"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            6..81
                     /locus_tag="OO349_004035"
     tRNA            6..81
                     /locus_tag="OO349_004035"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Thr,seq:ggt)
     gene            121..236
                     /gene="rrf"
                     /locus_tag="OO349_004036"
     rRNA            121..236
                     /gene="rrf"
                     /locus_tag="OO349_004036"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(299..1057)
                     /locus_tag="OO349_004037"
     CDS             complement(299..1057)
                     /locus_tag="OO349_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCR5868904.1"
                     /translation="MSQILLQPANAMITLENVHKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQATPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(1065..2168)
                     /locus_tag="OO349_004038"
     CDS             complement(1065..2168)
                     /locus_tag="OO349_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCR5868905.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAAWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2178..>2807)
                     /locus_tag="OO349_004039"
     CDS             complement(2178..>2807)
                     /locus_tag="OO349_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCR5868906.1"
                     /translation="FILAVVMAIVLSVGLFRFNKTYQIKTGQLRRSWPIAAVLIIGLP
                     LLAQWLFGAALHWDVPALQGFNFRGGMVLIPELAALTLALSVYTSAFIAEIIRAGIQA
                     VPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSSLATAIGYPDM
                     VSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVER"
BASE COUNT          762 a          738 c          676 g          631 t
ORIGIN      
        1 agcgtgctga tatggctcag ttggtagagc gcacccttgg taagggtgag gtccccagtt
       61 cgactctggg tatcagcacc actttttagg ttaaagttcg gcagattaga aaagaatttg
      121 tctggcggca gttgcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc
      181 cgtagcgccg atggtagtgt ggggtctccc catgcgagag tagggaactg ccagacatca
      241 aatcaagcga aaggccatcc gaaaggatgg cctttttgct tttcgaacta acattcaatt
      301 aatggattac ctgcgataaa aatgccctcg tacgctctga tttaggatgc gcaaaaaatt
      361 catcaggtgt agcttgttcc actatctccc cacgatccat aaaaattacc cggtcagcga
      421 cggtacgtgc aaaccccatc tcatgtgtta cacacaacat tgtcataccc gactgcgcca
      481 gcccaatcat cgtatccagc acctctttca ccatctccgg atcgagcgcc gacgttggtt
      541 catcaaacaa cataattttc ggcttcatac acagcgaacg cgcaatggca acgcgttgct
      601 gctgaccacc tgaaatctgt ccgggaaact tatgcgcatg ttcggcaatt ctcacccgct
      661 caaggtaatg catcgccaga gcttcagcct ctttcttagg catcttgcgt acccaaatcg
      721 gtgccagggt acagttctgt aaaacggtca gatgagggaa gagattgaaa tgctgaaata
      781 ccattcccac ttcctgcctg acgcgctcaa tattgcggat atcttcatta agttcgatgc
      841 catctacgac aatccgtccc tgttgatgtt cttccagatg attaatacaa cgaatggttg
      901 tcgatttacc ggaaccagaa gggccacaca gaacgatccg ttctcccggt tgcacggtta
      961 aatttatatt tttcaaaaca tggaattgtc cataccattt atggacgttt tccagcgtaa
     1021 tcatcgcgtt agcaggttgc agtaaaattt ggctcatagt gtcctcaatg cggtgtacgc
     1081 ccggtgttaa aacgtttttc cagatgttgg ctataacgtg acatgctgaa acaaaagatc
     1141 cagtagatca gcgcggcgaa aacataccct tccgtcgaca tacccaacca ggcgggatca
     1201 acggttgcct gctgaacgct actgaaaaga tcgaacaagc cgatgatgat cactaggctg
     1261 gtatctttga agagtgcgat gatggtattt accagcccag gaattaccag cttcaacgcc
     1321 tgtggcagaa taaccagccc ctgagttttc cagtaaccca acgccagcga ctctgccgct
     1381 tcatattgcc ctttaggcag cgcctgtaat ccacctcgca cgacttccgc aacatatgct
     1441 gactgaaaca ggatcacgcc aaccagcgcg cggatcagtt tgtcgatact ggtgccttct
     1501 gccataaaca acggcagcat gaccgaagac ataaacaata cggtaatcag cggtacgccg
     1561 cgccagaact cgataaaaat gaccgataag atacgcacaa tcggcatatg ggagcggcga
     1621 cctaatgcca gtaagatccc ccacggtagc gcccccgcaa tcccaacgga tgcaataatt
     1681 aaagttagcg tcagcccgcc ccattgccgg gtttcaaccc gctcaagacc aaaaaaaccg
     1741 ccatacatca gccaccagac aatcagtggg taaatcaccg cccaggccgc aatatagcga
     1801 ccgcgatgcg ggagtatttt ccagaacatt ggtgcgatcg atacaagccc aatcagtaat
     1861 gcgaggttaa ttcgccagcg ttggtcgtgt gggtaaagtc catacataaa ctgaccaaat
     1921 cgttcgtgaa tgaagaccca acaggcaccg gcttttgtgc agtcagcacg cgttgagcca
     1981 acccaattag cctgcaaaaa tgcccagttc agcaacggtg ggatcagttc ccacatcaac
     2041 catatgcagc caatagtcag caggctattg ctccagctgg agaacagatt ttttcgcacc
     2101 cacaccatcg cgcggcttga gttatggctc gccgggcgcg agggctgaga cagcaatact
     2161 tttgtcatca tgactcctta gcgttcaacg atcgcgatgc ggcggttata gatattcatc
     2221 agcagcgaga tagtcaggct gataatcaga tagaccgaca tggtcatggc tatcgtctcg
     2281 atggcttgcc ccgtctggtt cagcacggtg ccagcaaaca gcgaaaccat atcgggatag
     2341 ccaatagcgg tggcaagaga ggagtttttg acgatgttga gatactggct ggttaacggc
     2401 ggaataatta ctcgcagtgc ctggggaata atgacctggc gtagcgtaac cgggttgggt
     2461 aaccccagtg accgagccgc ttcatgttga ccataaggca ctgcctggat cccagcgcgg
     2521 ataatctcgg cgataaatgc agaggtataa accgaaagtg ccagcgttaa ggctgccagt
     2581 tcaggaatta aaaccattcc gccgcggaag ttaaagcctt gtagggctgg gacatcccag
     2641 tgtagtgctg cgccaaaaag ccattgcgcc agtaaaggca aaccaatgat caacactgcg
     2701 gcgatcggcc aggagcggcg cagttgtccg gtctttatct ggtatgtttt attaaagcgg
     2761 aatagcccaa cagaaaggac tatagccata acaacagcca ggataaa
//
LOCUS       DAJTQY010000214         2690 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.218, whole genome shotgun sequence.
ACCESSION   DAJTQY010000214 DAJTQY010000000
VERSION     DAJTQY010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.218"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            230..769
                     /locus_tag="OO349_004040"
     CDS             230..769
                     /locus_tag="OO349_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015870862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HCR5868907.1"
                     /translation="MSHGGWKEISHPQTLFTRQKGDGWTVVPKNLRIDFYTKDNDFTK
                     GLSVLSEVNKRHTEAQKGLTPSLNISKDDLKVLANARNIPQSKLEEEMMSQAIYRKEY
                     ATSNEKIKNYALYYHEQAQNIIQRHQDGLGNKNIDIAFITDKNHKKHLSDIFKVINES
                     GVKYDVIHFGACRVNREEK"
     gene            complement(995..1318)
                     /locus_tag="OO349_004041"
     CDS             complement(995..1318)
                     /locus_tag="OO349_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nikA protein"
                     /protein_id="HCR5868908.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLR
                     AAALGKNVNSLTDDRVLKEVMRLGALQKKLFIDGKRIGDREYAEVLIAITEYHRALLS
                     RLMAD"
     gene            1604..1798
                     /locus_tag="OO349_004042"
     CDS             1604..1798
                     /locus_tag="OO349_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HCR5868909.1"
                     /translation="MNKQQQTVLNMAGFIKSQSLTLLEKLDALDADEQAAMCEKLHEI
                     AEELQNSIQTRFEAESGTGV"
BASE COUNT          857 a          665 c          579 g          589 t
ORIGIN      
        1 aaaaaaaaca ataaaaaaca ccgcaaaaat ggattgttaa caaatgaaaa tactcaattc
       61 gaatctcata acaaacatat catcaacaat aagaaatcac cttctaacat catccaaaaa
      121 tataaaaaca cacccatcac caaacatgga cgtaacaagt catcgtcgtt tattccagaa
      181 agacggaata ggattgagcc aaacaggaaa agcaacacaa cttgtcatta tgtcgcatgg
      241 tggatggaaa gagatctccc atccacaaac actattcacg cgtcaaaaag gcgatggatg
      301 gactgtagtc cccaaaaacc taagaattga tttttataca aaagacaacg acttcacaaa
      361 gggactaagc gttctttcag aggtaaacaa acgccatact gaagctcaaa aagggttaac
      421 accatcacta aacataagca aagatgacct aaaagttcta gcgaacgcgc gcaatatacc
      481 tcaatcaaaa ttagaagaag aaatgatgtc acaagccata tatagaaaag aatacgcaac
      541 aagtaatgag aagataaaaa actatgctct atattaccat gaacaagccc aaaatattat
      601 acaaagacat caagatggct taggaaacaa aaatattgat atagcattta taactgataa
      661 aaatcataag aagcaccttt ctgacatatt taaagtaata aatgaatcag gagtaaaata
      721 tgatgtaatt cactttggcg cttgccgagt aaatagagag gagaaataat catcatttac
      781 cataatccct taattgtacg caccactgaa acgcgctgaa cgcgattctg acgcagacac
      841 cacaaaaata gatgttttac cccggaaaaa caggaaggat gcgtcaggat cgttttcagc
      901 tcgctgcata gctatgcatg aaagcgaatg gagatcactt tgggagctta ccgtgttcat
      961 accgtctgtt ttcaacagtt ttctccccgg gaagctaatc tgccataagc ctggataaca
     1021 gggcacggtg atactccgta atagcgatca gcacctccgc atactccctg tccccgatac
     1081 gcttgccgtc gataaagagt tttttctgta acgcccccag tctcataact tctttcagca
     1141 cccgatcatc agtcagtgag ttaacgttct taccgagagc tgccgcccgt aaaaaaccag
     1201 aaacggtctt gccgcattca gcagcccttt ttctgatttc ctggtcttct tctgccgtca
     1261 gacgaacagg gcgactgata gcccgcctgc gcgtattact tccacttcgt tttgtcataa
     1321 aacatggctc cgtatctgac acggggtgtc ggggcgaagc cctgaccagg gtaattgtaa
     1381 tagcgtgcat gtatgcgcgg tataacaatt acacatcctg tcctgtcagc aagttcgaat
     1441 taccgacaaa ggcacggcag tttttttggc agtgacagta ttgtcaggca gaaatacccg
     1501 tcaatgcgtc agacggcaga ataccggcgg taaaacagtc gaaaagtggt acgcgatata
     1561 atcacacaac gcagatgaca gacagaacgg agaaaacaga aagatgaaca agcagcagca
     1621 aaccgtactc aacatggcgg gattcataaa aagccagagc ctgacgctgc tcgaaaagct
     1681 ggacgcactc gatgccgacg aacaggccgc catgtgtgag aagctgcacg aaatcgcgga
     1741 agaactccag aacagcatac agacacgctt tgaagcggaa agtggcaccg gggtataaca
     1801 gtgtatcctg ctccagacag gatgcagaac ggtataccgg tgcgtaagga gaaaatgtca
     1861 cataaggcgc tcttccgctt cctcgctcac tgactcgcta cgctcggtcg ttcgactgcg
     1921 gcgagcggtg tcagctcact caaaggcggt aatgcggtta tccacagaat caggggataa
     1981 cgccggaaag aacatgtgag caaaaagccg aaggcagggg caaaggccgc gcaggtggcg
     2041 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     2101 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     2161 cgctctcctg ttccgaccct gccgtttacc ggatacctgt ccgcctttct cccttcggga
     2221 agcgtggcgc tttctcatag ctcacgctgt tggtatctcg gcccggtgta ggtcgttcgc
     2281 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     2341 aactatcgtc ttgagtccaa cccggtaaga cacgaattat cgccactggc agcagccact
     2401 gataaccgaa ttagaggagt aagacttgaa gtcatgcgcc tgttaaggct aaactgaaag
     2461 aacaagtttt ggtgactgcg ctcctccaag ccagttacct cggctcaaag agttgatagc
     2521 tctgagaacc ttcgaaaaac cgccttgcag ggcggttttt tcgtttacag gcagcagatt
     2581 acgcgcagaa aaaaaggatc tcaagaagaa cctttaattt tttttcacac ttggtatcct
     2641 actttaaccc ataaaataaa cacttacacc gacacaccct taaacaaaag
//
LOCUS       DAJTQY010000215         2555 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.219, whole genome shotgun sequence.
ACCESSION   DAJTQY010000215 DAJTQY010000000
VERSION     DAJTQY010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2555
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.219"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            <1..488
                     /gene="capU"
                     /locus_tag="OO349_004043"
     CDS             <1..488
                     /gene="capU"
                     /locus_tag="OO349_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HCR5868910.1"
                     /translation="FMLNLLFHLKMNGRQFCWLIVGSGSPELREHLQYQIDSMGMHDD
                     VFIADNVFPAAPVYRVASLVVLPSENESFGMVLAEASAFSVPVVATQIGGIPEVIQNN
                     QTGTLLPASNKHAWMCALNDFFNDPGRFYQMARLAKQDIEERFDINKTALKILTLAKQ
                     K"
     gene            493..1443
                     /gene="virK"
                     /locus_tag="OO349_004044"
     CDS             493..1443
                     /gene="virK"
                     /locus_tag="OO349_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HCR5868911.1"
                     /translation="MFSISNLSFIGFLKRIIFSSDSLPGKWEHRKFRFMYILRCSINP
                     VVSIRYYYELRSLPCIEDILAIHPTLPARIHRPYLHKGGRAWTRGQYILEHYRFVQNL
                     PEKYSKFLFPQKSVSLVQFIGKDGENFDIQCSPSGFDREGELMLSLFYNKTVIARLTF
                     SVILTQNGHIAFIGGLQGAPKNTGPDVIRCATRACYGIFPKRIIFEAFCALMKACNIS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYRLPTQVIRKPLSD
                     IASKKRSEYRKRYALLDYIHETTIRSLDAYPVHSEHQNLN"
     gene            1508..2452
                     /gene="lpxM"
                     /locus_tag="OO349_004045"
                     /gene_synonym="msbB"
     CDS             1508..2452
                     /gene="lpxM"
                     /locus_tag="OO349_004045"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCR5868912.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWSTWFLLGMIAGISMFPPSFRDP
                     VLAKIGRWAGRLSKKARRRATINLSLCFPEKSDTEREIIVDKMFVTALQSIVMMAELA
                     IRGPEKFQKRVFWKGLEILEEIRHNNRNVIFLVPHGWSVDIPAMLLAAQGKKMAAMFH
                     QQRNPVIDYVWNSVRRKFGGRLHAREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFA
                     DFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKHLLTIEIRPPMDACIASADNKTI
                     ARQMNKTVEILVGPHPEQYVWVLKLLKTRKSNEADPYP"
BASE COUNT          738 a          487 c          577 g          753 t
ORIGIN      
        1 aatttatgtt gaatttactg ttccatttaa aaatgaatgg acgacagttc tgttggttga
       61 ttgtggggtc tggttcacct gaactgcggg aacatttaca gtatcagatt gacagtatgg
      121 gcatgcatga tgatgttttt attgctgaca atgtttttcc tgccgccccc gtatatcggg
      181 ttgccagtct ggtggttctg ccttcagaaa acgaatcttt tggtatggtg ctggcagaag
      241 catcggcatt ttctgtgcct gtagtggcca ctcagattgg tggaatccct gaggttattc
      301 agaacaacca gaccgggaca ttgttaccag caagtaataa gcacgcatgg atgtgcgccc
      361 tgaatgattt ttttaatgac cctgggcgtt tttatcagat ggctcgcctg gcaaaacagg
      421 atatagaaga gcggtttgat attaataaaa ctgcgttaaa aatactcaca ttagcgaagc
      481 aaaagtaaca atatgttttc tataagtaac ttatcattta tcggtttcct taaaaggatt
      541 attttttcct cagattcact accggggaag tgggaacaca gaaaatttcg gttcatgtac
      601 attttgcgat gttctataaa tccggttgtc agtattcgat attattatga actgcgttcc
      661 ttgccgtgca ttgaggatat tctggcaata caccccacgt tgccagcaag aattcatcgc
      721 ccttatttgc ataaaggggg gcgcgcctgg acccgtggac aatatattct tgagcattat
      781 cgctttgtgc aaaatttgcc agaaaaatat tctaaatttt tgtttccaca aaaatcggta
      841 tcattagtcc aattcatcgg gaaagatgga gagaattttg atatccagtg ttctcccagt
      901 ggttttgacc gggaaggaga gttgatgttg tcattgtttt acaataaaac agttattgcc
      961 cggttaacat tttcagtcat tctgactcaa aatggacaca ttgcttttat tggcggatta
     1021 cagggagccc ctaaaaatac cggacctgat gttattcggt gcgcaacccg ggcctgttat
     1081 gggattttcc caaaacggat tatttttgaa gcattttgtg cgttgatgaa agcttgcaat
     1141 atctctgagt gtctggcagt cagtgagcac agtcatgttt tccggcaatt gagatactgg
     1201 tatcagaaac gcaaaacctt tgttgcggtc tatagtgatt tctgggagtc tgtagcagga
     1261 aaaacctgtg gtgactggta cagattacca acccaggtga ttcgaaagcc gctaagcgat
     1321 atagccagta aaaaacgctc tgagtaccga aaacgatacg ctttgctgga ttatatccat
     1381 gaaactacta tccgctcttt ggatgcatat cctgtacact cagaacatca gaatttaaat
     1441 taaggtttga tgtattctct gaataaaaca gtaatgataa ttttggtaga ggcattcgca
     1501 ctaaataatg aaaaaatata aatctgagtt tattcctgaa tttaagaaaa attacctttc
     1561 ccctgtttac tggtctacat ggttcctttt gggaatgatt gcaggtattt caatgtttcc
     1621 cccttcattc agagatcctg tcttggcaaa aatagggcgt tgggcgggca gattgagcaa
     1681 aaaagctcgt cgcagggcga cgattaattt atcgctttgt ttcccagaaa agagtgatac
     1741 agaacgggaa ataattgtcg acaaaatgtt tgtcacagca ttgcaatcca tagtgatgat
     1801 ggcagaactg gcaattcgtg gtccggaaaa gttccagaaa cgagtgttct ggaaaggcct
     1861 cgaaattctt gaggaaattc gacacaataa tagaaatgtg atttttctgg ttccccatgg
     1921 ctggagcgtg gatattcctg caatgttgct ggcagctcag ggaaaaaaaa tggccgccat
     1981 gtttcatcag caacgaaatc cagtgattga ttatgtctgg aattcagtac ggcgtaaatt
     2041 cggggggcgc ttacatgccc gggaggatgg gataaaacca tttattcagt cagtacgcca
     2101 gggatactgg ggatattacc tcccagatca ggatcatggt cctgaataca gtgaatttgc
     2161 tgattttttt gcgacctata aagcgacatt accaatcatt ggacgtctga tgaacatcag
     2221 tcaggctatg attataccac ttttcccggt ttatgatgaa aaaaaacatc tactgactat
     2281 tgaaattcgg ccaccaatgg atgcttgcat tgccagcgcg gacaataaaa cgattgcccg
     2341 acaaatgaac aaaacagtgg aaattttggt ggggccacat ccggaacagt atgtctgggt
     2401 tttaaaattg ttaaaaacac gcaaatcaaa cgaagcggac ccgtaccctt gaaattataa
     2461 agtacaagta tttccactag ttgtttgtaa gcgtacagcc tgaaccgtct ggtcagaatc
     2521 tgacgaatta gacaaagtgg tgtccaccaa ataag
//
LOCUS       DAJTQY010000216         2391 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.222, whole genome shotgun sequence.
ACCESSION   DAJTQY010000216 DAJTQY010000000
VERSION     DAJTQY010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2391
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.222"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            <1..983
                     /gene="proB"
                     /locus_tag="OO349_004046"
     CDS             <1..983
                     /gene="proB"
                     /locus_tag="OO349_004046"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HCR5868913.1"
                     /translation="GHRIVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQ
                     LWEQLFSIYGIHVGQMLLTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAE
                     IKVGDNDNLSALAAILAGADKLLLLTDQKGLYTADPRRNPQAELIKDVYGIDDALRAI
                     AGDSVSGLGTGGMSTKLQAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQA
                     TPLENRKRWIFGAPPAGEITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICN
                     LEGRDIAHGVSRYNSDALRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            995..2248
                     /gene="proA"
                     /locus_tag="OO349_004047"
     CDS             995..2248
                     /gene="proA"
                     /locus_tag="OO349_004047"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HCR5868914.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALTQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
BASE COUNT          561 a          606 c          699 g          525 t
ORIGIN      
        1 ccgggcatcg gattgttatt gtgacgtcgg gcgcgatcgc cgccggacgt gagcacctgg
       61 gttacccgga actgccagcg actatcgcct cgaaacaact gctggcggcg gtagggcaga
      121 gtcgactgat tcaactgtgg gaacagctgt tttcgattta tggcattcac gtcgggcaaa
      181 tgctgctgac ccgtgctgat atggaagacc gtgaacgctt cctgaacgcc cgcgacaccc
      241 tgcgtgcgtt gctcgataac aatatcgttc cggtaatcaa tgagaacgat gctgtcgcta
      301 cggcagagat taaggtcggc gataacgata acctttctgc gctggcggcg attctggcgg
      361 gtgccgataa actgttgctg ctgaccgatc aaaaaggttt gtataccgct gatccgcgca
      421 gaaatccgca ggcagaactg attaaagatg tttacggcat tgatgacgca ctgcgcgcga
      481 ttgccggtga cagcgtttca ggcctcggaa ctggcggcat gagtactaaa ttgcaggccg
      541 ctgacgtggc ttgccgtgcg ggtatcgaca ccattattgc cgcgggcagc aagccgggcg
      601 ttattggtga tgtgatggaa ggcatttccg tcggtacgct gttccatgcc caggcgactc
      661 cgcttgaaaa ccgtaaacgc tggattttcg gtgcgccgcc ggcgggtgaa atcacggtag
      721 atgaaggggc aactgccgcc attctggaac gcggcagctc cctgttgccg aaaggcatta
      781 aaagcgtgac tggcaatttc tcgcgtggtg aagtcatccg catttgcaac ctcgaaggtc
      841 gcgatatcgc ccacggtgtc agtcgttaca acagcgatgc attacgccgt attgccggac
      901 accactcgca agaaattgat gcaatactgg gatatgaata cggcccggtt gccgttcacc
      961 gtgatgacat gattacccgt taaggagctg gccgatgctg gaacaaatgg gcattgccgc
     1021 gaagcaagcc tcgtataaat tagcgcaact ctccagccgc gaaaaaaatc gcgtgctgga
     1081 aaaaatcgcc gatgaactgg aagcacaaag cgaaatcatc ctcaacgcta acgcccagga
     1141 tgttgctgac gcgcgagcca atggccttag cgaagcgatg cttgaccgtc tggcactgac
     1201 gcccgcacgg ctgaaaggca ttgccgacga tgtacgtcag gtgtgcaacc tcgccgatcc
     1261 ggtggggcag gtaatcgatg gcggcgtact ggacagcggc ctgcgtcttg agcgtcgtcg
     1321 cgtaccgctg ggggttattg gcgtgattta tgaagcgcgc ccgaacgtga cggttgatgt
     1381 cgcttcgctg tgcctgaaaa ccggtaatgc ggtgatcctg cgtggtggta aagaaacctg
     1441 tcgcactaac gcggcaacgg tggcggtgat tcaggacgcc ctgaaatcct gtggcttacc
     1501 ggcgggtgcc gtgcaggcga ttgataatcc tgaccgtgcg ctggtcagtg aaatgctgcg
     1561 tatggataaa tacatcgaca tgctgatccc gcgtggtggc gctggtttgc ataaactgtg
     1621 ccgtgaacag tcgacaatcc cggtgatcac aggtggtata ggcgtatgcc atatttacgt
     1681 tgatgaaagt gcagagatcg ctgaagcatt aaaagtgatc gtcaacgcga aaactcagcg
     1741 tccgagcaca tgtaatacgg ttgaaacgtt gctggtgaat aaaaacatcg ccgatagctt
     1801 cctgcccgca ttaagcaaac aaatggcgga aagcggcgtg acattacacg cagatgcagc
     1861 tgcgctgaca cagttgcagg caggccctgc gaaggtggtg gcggttaaag ccgaagagta
     1921 tgacgatgag tttctgtcat tagatttgaa cgtcaaaatc gtcagcgatc ttgacgatgc
     1981 catcgcccat attcgtgaac acggcacaca acactccgat gcgatcctga cccgcgatat
     2041 gcgcaacgcc cagcgttttg ttaacgaagt ggattcgtcc gctgtttacg ttaacgcctc
     2101 tacgcgtttt accgacggcg gccagtttgg tctgggtgcg gaagtggcgg taagcacaca
     2161 aaaactccac gcgcgtggcc caatggggct ggaagcactg accacttaca agtggatcgg
     2221 cattggtgat tacaccattc gtgcgtaaat aaaaccgggt gatgcaaaag tagccatttg
     2281 attcacaagg ccattgacgc atcgcccggt tagttttaac cttgtccacc gtgattcacg
     2341 ttcgtgaaca tgtcctttca gggccgatat agctcagttg gtagagcagc g
//
LOCUS       DAJTQY010000217         2250 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.223, whole genome shotgun sequence.
ACCESSION   DAJTQY010000217 DAJTQY010000000
VERSION     DAJTQY010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.223"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            84..707
                     /locus_tag="OO349_004048"
     CDS             84..707
                     /locus_tag="OO349_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5868915.1"
                     /translation="MVLNNPYFEANAGGADLAIDNTGTRPVTVVINGGNFHRVSSARY
                     THTNIQVTSSGGGKVTVILNGTTFQSAGDYQPSAERPYWITGANCEVVDIGCVFTETT
                     SKVTSASALSVTRSGKINANGSIDVATGVSSVNVVSTGVYDVSFSHPLAAVASGYIVQ
                     ITPISAPDSVSCDVTYIGVDTFRVTLRNTLSGAGISSSFAFSITRLI"
     gene            complement(761..1933)
                     /locus_tag="OO349_004049"
     CDS             complement(761..1933)
                     /locus_tag="OO349_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCR5868916.1"
                     /translation="MHMIEINSLLLITSVILMSLLAVGLFDKISPINLVEHGRNNQID
                     GMRGFLAIFVLIHHAAIWNGYLSSGVWEAPSSNLLANLGQVGVSFFFMITGYLFFSKI
                     ISGDQDWTRLYVSRLLRLTPMFIVSLCLIFIIVGFKSGWRMQVSTEELFVSIMKWLPF
                     TALGMPNINDVKDSFTINAAVTWTLVYEWFFYFSLPVISALIKRKVSIYMVMISAISL
                     FVFILFFSKIHIASFLFGLLAFLLNKSKIVNGIAKAKVTPIIITAIMVFEMTYFKTTY
                     APLPLILCGITFIIIASGCDLYGILRLNITRKLGETTYSVYLLHGIFLYCLMTWIIPN
                     NYTENTFIILVSTTAFLITFTSCLTFKLIETPFIKLTKQTTTLVKELIPTLTNNNQ"
BASE COUNT          690 a          402 c          463 g          695 t
ORIGIN      
        1 cttgtgaagg aaatggtact cagggggatt ccagtacagg tggtgcgcaa ttagctataa
       61 atggccttaa cggttcgtgc gctttggtat taaacaaccc atactttgag gctaatgccg
      121 ggggggcaga ccttgctata gataatactg ggacgcgccc tgtaactgtg gttataaacg
      181 gtggaaattt tcatcgcgtg agcagtgcaa gatacacgca cacgaatatt caagtaacaa
      241 gctcaggtgg gggaaaagtt acagtcatac tcaacggcac aacgttccag agcgcagggg
      301 attatcagcc aagtgcagaa cgaccatact ggataacagg agctaattgc gaggtagttg
      361 acattgggtg tgtttttaca gaaacaacca gtaaggtaac atcggcttct gcattgtcag
      421 tcacacgctc gggaaaaatt aacgccaatg gtagtatcga cgttgctact ggtgtttcat
      481 cggttaacgt ggtttctaca ggtgtgtatg atgttagctt ttctcatccg ctcgcagcag
      541 ttgctagcgg ttatattgtg cagataacac ctatatctgc gcctgattca gttagttgtg
      601 atgttactta tattggcgta gatacattta gggtgaccct gcgcaatacg cttagcgggg
      661 caggaatctc cagttcgttt gcatttagca ttaccagact aatataaata cattcgccgc
      721 cccatttgga catatatgta gggcggctta tgtttattgt ttattgattg ttgtttgtta
      781 atgtgggtat taattctttt actaaagttg tggtttgttt tgttaatttg atgaatggcg
      841 tctctattaa tttaaaagtt aggcatgatg tgaacgtaat aagaaatgca gttgtgctta
      901 ccagtatgat aaatgtattt tcagtgtaat tattaggaat aatccacgtc attaggcaat
      961 aaaggaatat cccatgcaga aggtaaacgc tgtaggtagt ttctcctagc tttctggtta
     1021 tatttaatct taatattcca tacaaatcac aacctgatgc gataatgata aatgttattc
     1081 cacaaagaat cagtggtagc ggtgcgtaag ttgttttgaa atacgtcatt tcaaaaacca
     1141 ttatcgcagt tattataatt ggtgttactt ttgctttggc aattccattg actatctttg
     1201 atttatttag caaaaatgcc aggagcccaa aaagaaatga tgctatgtgt attttactga
     1261 aaaataaaat gaaaacaaat agcgatattg cgctaatcat aaccatgtaa atactgactt
     1321 ttctttttat gagcgcggaa attaccggaa gagaaaaata aaagaaccat tcatatacaa
     1381 gtgtccatgt tacagcggca ttgatagtaa atgaatcttt tacgtcatta atgttcggca
     1441 tacctagcgc agtgaatggc aaccacttca ttattgacac aaaaagctct tctgtggata
     1501 cctgcattct ccatccagac ttaaaaccta caatgataaa aatgagacat aggctaacta
     1561 tgaacattgg ggttaatcgt agtaatcttg atacataaag ccttgtccag tcctgatctc
     1621 cagagataat ctttgaaaag aacagataac cagtaatcat aaaaaagaat gacacaccaa
     1681 cttggcctaa atttgctaac agatttgatg aaggtgcttc ccatactcca gatgacaagt
     1741 atccattcca aattgctgcg tgatgaataa gcacgaaaat tgctaagaac ccccgcattc
     1801 catctatttg attattcctg ccgtgctcaa caagatttat tggggaaatt ttatcaaata
     1861 accccacagc taataacgac atcaatatca cggatgttat taataataaa ctgttaattt
     1921 caatcatatg catgctctat tacgcaatta aaatttctcc aagtatactg acttttagtt
     1981 aatgatcata tagttatgtg taactgatga aacaaaacgg agacacacca agctttgcac
     2041 tggattgcaa ggctttgtgc tcttctgtga atgtgtggct atatgtttga agatcgttgt
     2101 gccgtatttg tgacatatat atggcaacat catgcatcaa ctttctgttt gtgtcatcaa
     2161 ctatagctta gtgaatgcgg ttaatgcttg ctaaaacaga tagttatgat tggtgctaca
     2221 gattcgtaat gcgaaggtcg taggttcgac
//
LOCUS       DAJTQY010000218         2236 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.224, whole genome shotgun sequence.
ACCESSION   DAJTQY010000218 DAJTQY010000000
VERSION     DAJTQY010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2236
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.224"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            65..1543
                     /locus_tag="OO349_004050"
                     /pseudo
     CDS             65..1543
                     /locus_tag="OO349_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001659238.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            1540..>2236
                     /locus_tag="OO349_004051"
     CDS             1540..>2236
                     /locus_tag="OO349_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HCR5868917.1"
                     /translation="MKGQIITVALILLGHTFLTPAVQAIGFDYYNDHGVMSYGKGYWG
                     DKKIIAQFPEMSRAGLRLVKNISGKTGYIPSDEISIENEEYHWVTDGRVILWRGKIVS
                     NPPGTLTVDIASFQAMGRFAVDKYSLYFDGQRTESNSGASRVELATLKAIEGNSTTLV
                     DSKNLYLSGRRQGSSSDVTVLEKRWWGINPRLMSVNRNSYSNDLLIRSGQNIYLNGVH
                     LTANADSFEIIRWI"
BASE COUNT          580 a          531 c          553 g          572 t
ORIGIN      
        1 tactcgtgag gatttatgtt ctgctggcag tttcgtgatc aacacctcgc gccaggatgg
       61 ctttatgcac tatcgcaatg tggtttgttt gttgtcttgt tcattgtttt tatcgtcggc
      121 atggggatgt cggctttccg cacctgaaca taatatttac cagaaacagg gaaaaggcgt
      181 ggtgtacctg cgcccttata aagaaacaaa tctttctcta cctgacgtga attacaagag
      241 tttgcgtcgg ttacccaatt tattaataga cccgacaaca ctagacgaat gggataaaga
      301 accgcctctg actgatttga caaccgacta cctctatgag ggcgcacagg cctggtatcc
      361 tcattattca tggcatagcg atggtcgcaa tatcctctac gcgggtgaag ttgtgcaaaa
      421 ccctcccggc acacctcccg tcgatgtcgc ctcgttcaag gcatggggtg attttgccgc
      481 agataaacac agtctctatt ttgaaggcaa gcgcaccgat gacaatggtg gtggaaatag
      541 cttggatatc aaaacgctgc atcaggtgga attccggcct ccctgggatc ctgacttact
      601 ggggctgata ttacgtgacg ctaatttctt gtatatcaat gggcatcgcc tcgctgaccc
      661 cgagagtttc cgtgtgctgg cgcagaagtc ctgggatcag cgaggaaagt tttccaccac
      721 gtttaacccc tgcattgccg taccatttgg cccctgggat accctggctc gtacacggac
      781 caaaatcctg ctcaacggcg aacagcttga tgccgacccg gacacctttt ccgtcgtgcg
      841 ctggatgccc ggctcactct tgacctggcg tgataaaaac gggctgcagc gtaaagtcct
      901 cgacaaggaa aatctggcgt gggatgaaga tttaacaaag cactgtctgg atttttctct
      961 gctggaaaag aaagtgttct ggcgtaaagg acctgcttgt aaacaggaag aattacccgg
     1021 actcgatccg gagcagtttc aacccatcag tgatgctgtc gcccagcatc aggactcgct
     1081 ttataccatc atcgaaacag agtctggtga ccgtaagctg gagatcgtga aacttgatga
     1141 tcccaatctt attatcaaca aacgtttcaa cgccgggaaa cgccacggct atttacttac
     1201 gcgtgccgaa gggtagccat accattccgg tttacacgtg tttgaatctg acggaccgct
     1261 gatcttactg gataaccgtt ctccggatga acgcgaagcc catcttaatg accatccctt
     1321 tttgcgcaga tggtatgccc gcgataaccg ctacatttac agctttgatg gcgcgcagct
     1381 ctggcgatac cgcaccgctg atccgaaaca agttcgctta atctggaagg aacaacattc
     1441 gggatatgtc tatggcgtaa attacaaaac gggatatctg gacggaaaaa ttactgatga
     1501 cggcgaattt attcctgccc cgcgcaatga ggcgacaaaa tgaagggtca aataatcaca
     1561 gttgcactca tcctgttggg acacacattt ctgacgcctg ctgtacaggc catagggttc
     1621 gactattaca atgatcacgg tgttatgtct tacggtaaag gttattgggg ggataagaaa
     1681 atcattgccc agtttccgga gatgagcagg gccggtttgc gccttgtgaa aaatatctct
     1741 gggaaaacgg gttatatacc ttccgatgaa ataagcatag aaaatgagga ataccactgg
     1801 gtgacagacg gccgcgttat tctctggcgt ggcaaaatcg ttagcaatcc accgggaaca
     1861 ctcactgtcg atattgccag ctttcaggct atgggccgct tcgcggtcga taaatatagc
     1921 ctctattttg acggacagcg caccgaaagt aatagcggcg cgtcacgtgt tgagctggcg
     1981 acactaaaag ctatcgaagg taactctacc acgctggtgg atagtaaaaa tctctacttg
     2041 tccggtcgac gtcaggggag cagcagtgat gttactgtat tagaaaaaag atggtggggt
     2101 attaatccac gtcttatgag tgtaaacaga aattcatatt ccaatgatct gcttatccgc
     2161 agtgggcaga atatttattt aaatggcgtt caccttacgg cgaatgcaga ctcatttgag
     2221 ataattcgct ggatac
//
LOCUS       DAJTQY010000219         2231 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.225, whole genome shotgun sequence.
ACCESSION   DAJTQY010000219 DAJTQY010000000
VERSION     DAJTQY010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.225"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            51..2231
                     /locus_tag="OO349_004052"
                     /pseudo
     CDS             51..2231
                     /locus_tag="OO349_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
BASE COUNT          597 a          496 c          562 g          576 t
ORIGIN      
        1 gtgacccgat aaaaatgacg ctgtaagcaa gggataagga taggcgtatt atgatgacga
       61 ccagaatagt ggttggcctc acggcaggga cgtgtctgat tttctcgcaa aacctgatgg
      121 ccgaggtcag tgtattcaat ccggcgcttc tggaaatcaa ccatcaatcc ggagtcgata
      181 ttcgccagtt taatcgggca aacctgatgc ccccaggtgt ttatagcgtt gatattttta
      241 tcaacggtaa aatgtttgaa cgtcaggatg tgacatttgt tcaggataat ccagatgctg
      301 atctgcacgc ttgctttatt gccattaaaa aaacactgtc ctcctttggc ataaaagttg
      361 atgcgctcaa atcgttcaat gatgtggatg agacggtttg cctcgatcct gctccacgta
      421 ttgaaggctc atcctggcag tttgacagtg ataaattgca gctgaatata tccattcacc
      481 aaatctacat ggacgcgatg gcttatgatt acatcagccc cacgcgttgg gatgagggga
      541 ttaatgcgct caccatcaac tacgattttt ctggttcaca tacactacgt tcagattatg
      601 gttcacaaga gacagatacc agttatctca atctgcgcaa tggactgaat attggaccgt
      661 ggcggctacg taattacagt actttaaaca ccagcgatgg ccgtgcggaa tacaactcca
      721 ttagtacctg gatacagcgc gatattgccg cgttaagaag ccagattatg attggtgata
      781 cgtggacggc gagcgatatt ttcgacagta cgcaaattcg cggcgcgcgt ttgtatactg
      841 ataacgatat gctacccgcc agccagaatg gctttgctcc tgtggttcgt gggattgcaa
      901 agtccaacgc caccgtcatc attcggcaga atggctacgt gatttatcag tcagccgttc
      961 cacaaggtgc ttttgagatc accgatctca acaccgcaag tacaggtggc gatttggacg
     1021 taaccatcaa agaagaagac ggtagcgaac aacgattcac ccaaccttat gcttcattgg
     1081 cgattcttaa acgtgaaggt ctgacagatg ttgatgtcag cgtgggtgaa ttgcgcgatg
     1141 aagacggatt tacaccggac gtccttcagg cgcaaatact tcatggtttt tcccacggga
     1201 tcactttata tggaggtatg caggctgctg aaaattatgg ttctgcagct ctgggtgtcg
     1261 gtaaagatct tggcgctttg ggcgcaattt ctttcgatgt gacacatgct cgtgcgaatt
     1321 ttagccatga tgatacagaa acgggtcagt catatcgctt tctctattca aaactatttg
     1381 acgacacaga cactagcttg cgcctggttg gctatcgtta ctccaccgag ggctactata
     1441 ccactcaatg agtgggcatc gcggcgcaac agccctgaag acttttggga aacaggtaac
     1501 cgacgtagtc gcgtggaggg aacgctaacg cagtcgttgg ggagagatta tggcaattta
     1561 tacctgacat taagccggca acaatactgg cataccgatg atgtcgaacg attaatgcaa
     1621 tttggctaca gcagtagctg gaagcgtctc tcgtggaacg tctcctggag ttattccaat
     1681 actgccagac aggggacggg gaacaaccat gccagtgata acaccagtga gcagatctac
     1741 atgctctctt tatctgttcc tttatcgggc tggtggggta atagttacgc cacctattct
     1801 gtttcgcaaa acgataattc cggtagctca catcaactcg gactcagcgg tacggcgctg
     1861 gaaagaaata acctttcatg gaatttaatg cagtcctata acagtcatga tgatgaggtt
     1921 ggcggtaata tgtccctgac ctatgatggc tcttatggca cggtgaacgg cagctataac
     1981 tacagccaaa attcccagag gctgaattat ggtatcagag ggggaattct ggcacacagc
     2041 gaaggggtaa cgttaagtca ggagttaggt gaaactattg ctcttgttaa agcacctggg
     2101 gccgccgggt tagaaataga taatatgcgc ggtgctgcga cggactggcg cggctatacg
     2161 gtcaagacac agctaaaccc ttatgatgaa aatcgggtag caatcagcga taactatttc
     2221 tcgaagtcga a
//
LOCUS       DAJTQY010000220         2096 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.226, whole genome shotgun sequence.
ACCESSION   DAJTQY010000220 DAJTQY010000000
VERSION     DAJTQY010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.226"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            116..1153
                     /gene="curA"
                     /locus_tag="OO349_004053"
     CDS             116..1153
                     /gene="curA"
                     /locus_tag="OO349_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HCR5868918.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATE
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHATEVLGFDVCLDHHADDFAEQLAKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYREDITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            1351..2016
                     /gene="mcbR"
                     /locus_tag="OO349_004054"
     CDS             1351..2016
                     /gene="mcbR"
                     /locus_tag="OO349_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HCR5868919.1"
                     /translation="MPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVRQSQLDEINRIRYELELMAVALAVE
                     NLSSQDIAELQELLGKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMLILCEMIEQLWI
                     RMGPALHYLYEAINPAELRERIENYHLLLAALKAKDKEGCKHCLAEIMQQNIAILYQQ
                     YNR"
BASE COUNT          577 a          444 c          560 g          515 t
ORIGIN      
        1 gattcctcga tgaaattatt gcgtgttgca gaggggatgt gacggctgca aacaaaacca
       61 atcccgtgag cctgctacta ttgatggatt catgccaaaa gcacaggaga gcattatggg
      121 gcaacaaaag cagcgtaatc gacgttgggt tctggcctcg cgtccacatg gcgcaccggt
      181 tccggagaat ttccgtcttg aagaagatga tgtcgccaca ccgggtgaag gacaggtgtt
      241 actgcgcaca gtttatttgt ccctggaccc gtatatgcgt ggacgtatga gcgatgagcc
      301 atcttattca ccgccggttg atattggcgg cgtgatggtc ggcggtacgg tgagccgtgt
      361 cgtggagtcg aatcatcctg attatcagcc tggcgactgg gtgctgggct acagtggatg
      421 gcaggactat gacatatcca gtggtgatga cctggtgaaa cttggcgatc atccgcaaaa
      481 tccatcgtgg tcgctgggtg tgctggggat gccaggcttt accgcttata tggggctgct
      541 ggatatcggt cagcctaaag agggcgaaac gttggtggta gctgcggcga cagaaccagt
      601 gggggcgacg gtggggcaaa tcggcaaact taaaggttgc agagtggtgg gggtagccgg
      661 tggcgcggaa aaatgccgcc atgctaccga ggtgttaggc ttcgatgttt gccttgatca
      721 ccacgcggac gattttgccg aacaactggc gaaagcgtgc ccaaaaggta ttgatatcta
      781 ttatgaaaac gtgggcggta aggtatttga tgcagtgcta ccattactta atacatctgc
      841 gcgcattccc gtttgtggct tagtgagcag ctataacgct acagagctac cacccggtcc
      901 ggatcgttta cccctgttga tggcaacggt gctgaaaaaa cgtattcgct tgcaagggtt
      961 tattatcgct caggattatg gtcaccgcat ccatgagttt cagaaggaga tggggcaatg
     1021 ggtgaaagag gataaaatcc actaccgcga agatattact gatggtttag agaatgcgcc
     1081 acagacgttt atcggcctgc tgaagggtaa aaacttcggc aaagtggtga tccgcgtcgc
     1141 gggtgatgat taaagtttca acggcggcgt aagccgccat aaaaatccag gcaaaaagct
     1201 tattttcagc tttaattaac taaccacgtc attgttctgc tgctaataac aacaaaggtg
     1261 ggtggtttat gattattcac tttaatacac taggtgaact ccttctgcca tgcaggcagg
     1321 gttggacaga aaacacgcga aggaattaaa atgcctggaa cgggaaaaat gaaacatgtc
     1381 agtttgactc tgcaggttga gaacgacctg aaacatcagc ttagtattgg cgcattaaaa
     1441 cctggcgcac gcctgattac taaaaatctg gcggagcaat taggtatgag tattacacct
     1501 gtgcgtgaag cattattacg tctggtttcg gtgaatgcgc tttctgtcgc acctgcacaa
     1561 gcatttacag taccggaagt gaggcaaagt cagttagacg aaatcaatcg gatccgctac
     1621 gaactggaat taatggcagt tgccctggct gttgaaaacc tctcctcgca agacatagca
     1681 gaacttcagg aattgctcgg gaaattacag caggcgcaag aaaagggcga catggaacaa
     1741 atcattaatg tgaacaggct atttcgctta gcgatttatc atcgctcaaa tatgctcatc
     1801 ctgtgtgaga tgattgagca actgtggatc aggatgggac ctgctttaca ttatctttat
     1861 gaagcgatta acccagcgga attacgggag cgcatagaaa actatcatct attactcgct
     1921 gcgttaaaag caaaagacaa agagggatgc aaacattgtc ttgctgaaat tatgcaacaa
     1981 aatattgcta ttttatacca gcaatacaat cgttaaatta aatgcccggc aaataccggg
     2041 catcaagtaa ttactcaaat cgccacgcaa tattcatgcc gacaccataa tttcgt
//
LOCUS       DAJTQY010000221         2035 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.227, whole genome shotgun sequence.
ACCESSION   DAJTQY010000221 DAJTQY010000000
VERSION     DAJTQY010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.227"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            <1..>2035
                     /gene="sat"
                     /locus_tag="OO349_004055"
     CDS             <1..>2035
                     /gene="sat"
                     /locus_tag="OO349_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine protease autotransporter toxin Sat"
                     /protein_id="HCR5868920.1"
                     /translation="GMMIYKNDKTFRNLEIFGDSGSGAYLYDNKLEKWVLVGTTHGIA
                     SVNGDQLTWITKYNDKLVSELKDTYSHKINLNGNNVTIKNTDITLHQNNADTTGTQEK
                     ITKDKDIVFTNGGNVLFKDNLDFGSGGIIFDEGHEYNINGQGFTFKGAGIDIGKESIV
                     NWNALYSSDDVLHKIGPGTLNVQKKQGANIKIGEGNVILNEEGTFNNIYLASGNGKVI
                     LNKDNSLGNDQYAGIFFTKRGGTLDLNGHNQTFTRIAATDDGTTITNSDTTKEAVLAI
                     NNEDSYIYHGNINGNIKLTHNINSQDKKTNAKLILDGSVNTKNDVEVSNASLTMQGHA
                     TEHAIFRSTASHCSLVFLCGTDWVTVLKETESSYNKKFNSDYKSNNQQTSFDQPDWKT
                     GVFKFDTLHLNNADFSISRNANVEGNISANKSAITIGDKNAYIDNLAGKNITNNGFDF
                     KQTISTNLSIGETKFTGGITAHNSQIAIGDQAVVTLNGATFLNNTPISIDKGAKVIAQ
                     NSMFTTKGIDISGELTMMGIPEQNSKTVTPGLHYAADGFRLSGGNANFIARNMASVTG
                     NIYADDAATITLGQPETETPTISSAYQAWAETLLYGFDTAYRGAITAPKATVSMNNAI
                     WHLNSQSSINRLETKDSMVRFTGDNGKFTTLTVDNLTIDDSAFVLRANLAQAD"
BASE COUNT          747 a          359 c          401 g          528 t
ORIGIN      
        1 acggcatgat gatttataaa aatgataaaa catttcgtaa tcttgaaata ttcggagaca
       61 gcggctctgg agcatactta tatgataaca aactagaaaa atgggtatta gtcggaacaa
      121 cccatggtat tgccagcgtt aatggtgacc aactgacatg gataacaaaa tacaatgata
      181 aactggttag tgagttaaaa gatacctata gtcataaaat aaatctgaat ggcaataatg
      241 taaccattaa aaacacagat ataacattac accaaaacaa tgcagatacc actggtactc
      301 aagaaaaaat aactaaagac aaagatattg tgttcacaaa tgggggaaat gttctgttta
      361 aggataattt ggattttggt agcggtggta ttatctttga cgaaggccat gaatataaca
      421 taaacggtca gggatttaca tttaaaggag caggaattga tatcggaaaa gaaagcattg
      481 taaactggaa tgcattgtat tccagtgatg atgttttaca caaaataggc cccggtactc
      541 tgaatgttca aaaaaaacag ggggcaaata taaagatagg tgaaggaaat gttattctta
      601 atgaagaagg aacatttaac aatatatacc ttgcaagcgg aaatggtaag gtaatactaa
      661 ataaagataa ttcccttggc aatgatcaat atgcggggat attttttact aaacgtggtg
      721 gcacgctaga tttaaatgga cacaatcaga cttttactag aattgccgcc actgacgatg
      781 gaacaacaat aactaactca gatacaacga aagaagccgt tctggcaatc aataacgaag
      841 actcctacat atatcatggg aacataaatg gcaatataaa actaacgcac aatattaatt
      901 ctcaggataa gaaaactaat gcaaaattaa ttctggatgg tagtgtcaac acaaaaaatg
      961 atgttgaagt cagtaatgcc agtcttacca tgcaaggcca tgcaacagag catgcaatat
     1021 tcagaagcac agcgagtcat tgctccctgg tatttctttg tggaacggac tgggtcaccg
     1081 ttttgaaaga aacagagagt tcatataata aaaagttcaa ttctgattac aaaagtaata
     1141 atcagcagac ctcatttgat cagcctgact ggaaaaccgg ggtgtttaaa tttgatacat
     1201 tacacctgaa caatgctgac ttttcaatat cacgcaatgc caatgttgaa ggaaatatat
     1261 cagcaaataa atcagctatc acaatcggcg ataaaaatgc ttacattgat aatcttgcag
     1321 ggaaaaatat tactaataat ggttttgact tcaaacaaac tatcagtact aatctatcca
     1381 taggagaaac taaatttaca ggtggcatca ctgcacataa cagccaaata gccataggtg
     1441 atcaagctgt agttacactt aatggtgcaa cctttctgaa taatactcct ataagtatag
     1501 ataaaggagc aaaagttata gcacaaaatt ccatgttcac aacaaaaggt attgatatct
     1561 ccggtgaact gactatgatg ggaatccctg aacagaatag taaaactgta acgccgggtc
     1621 tccactacgc tgctgatgga ttcaggctga gtggtggaaa tgcaaatttc attgccagaa
     1681 atatggcatc tgtcaccgga aatatttatg ctgatgatgc agcaaccatt actctgggac
     1741 agcctgaaac tgaaacaccg actatatcgt ctgcttatca ggcatgggca gagactcttt
     1801 tgtatggctt tgataccgcc tatcgaggcg caataacagc ccccaaagct acagttagca
     1861 tgaataatgc gatctggcat ctaaatagcc agtcatcaat taatcgtcta gaaacaaaag
     1921 acagtatggt gcgttttact ggtgacaatg ggaagtttac aacccttaca gtggacaacc
     1981 ttactataga tgacagtgca tttgtgctgc gtgcaaatct ggcccaagca gatca
//
LOCUS       DAJTQY010000222         2033 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.228, whole genome shotgun sequence.
ACCESSION   DAJTQY010000222 DAJTQY010000000
VERSION     DAJTQY010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.228"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(<1..363)
                     /locus_tag="OO349_004056"
     CDS             complement(<1..363)
                     /locus_tag="OO349_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5868921.1"
                     /translation="MDVDVSTSVAGNKPQRIRRIQTVTLVLLFMAGIVNFLDRSSLSV
                     AGEAIRGELGLSATEFGVLLSAFSLSYGFSQLPSGILLDRFGPRIVLGAGLIFWSLMQ
                     ALTGMVNSFSHFILMRIGL"
     gene            complement(430..1644)
                     /locus_tag="OO349_004057"
     CDS             complement(430..1644)
                     /locus_tag="OO349_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate dehydratase family protein"
                     /protein_id="HCR5868922.1"
                     /translation="MKIIAADVFVTCPGRNFVTLKITTESGLCGLGDATLNGRELSVA
                     SYLKDHLCPQLIGRDASRIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYG
                     LAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIGYIRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPYSWRFEEGYMHPGDEPGLGISFDEKLAAKYPYDPEYLP
                     VARLEDGTLLNW"
BASE COUNT          634 a          493 c          412 g          494 t
ORIGIN      
        1 cagaccgata cgcattaaaa taaagtggct aaaagaatta accattcctg ttaatgcctg
       61 cattaatgac cagaatatta agcctgcgcc taaaactatt cgtgggccaa agcggtctag
      121 caaaataccg gaaggtagtt gtgaaaaacc ataagacaga gaaaatgcag aaagcaaaac
      181 accaaactct gtcgccgata atcctaactc gccacgaatc gcttcgcctg ccacgctcaa
      241 tgacgaacgg tcgagaaaat taacgatccc cgccataaat aataaaacca gggtcactgt
      301 ctgaatacga cgaatccgtt gcggtttatt acccgcaaca ctggttgaca catcaacgtc
      361 catttttagc tccttgagcg gaattaatcg atttgatgac tattcagaaa ataattaagc
      421 gaaccgttat taccagttca acaatgtgcc atcttccagt cgtgcgacgg ggagatactc
      481 cgggtcatac ggatatttcg ccgccagttt ctcatcgaaa gaaataccca gccccggttc
      541 atcgcctggg tgcatataac cttcctcgaa gcgccagctg tacgggaaaa cttcaagcat
      601 ttgttcggaa tagcccatgt actcctgaac gccaaaattt ggtacccata ggtcaaaatg
      661 cagcgccgcc gcatgacaaa caggcgataa atctgaaggg ccatgcgaac cggtacgtac
      721 ctgataaagc gatgcaaaat cggcaatccg ccgcatcccg gtaatgccgc ctgcatgggt
      781 gatcgtcgtg cgaatatagc caatcagctg ctcttcgatt aactgcttgc agtcccagat
      841 actgttaaac acttcaccga ccgcaatcgg cgtcacggta tgctggcgaa tcagacgaaa
      901 acattcctgg ttctcggcag gcgtcggatc ttccatccag aacaggcggt aatcctcaat
      961 gctcttccca aagcgcgccg cttcgatggg ggtcaggcga tggtgcatgt catgcaacag
     1021 atgttcgtta aaaccgaact tattacgcac cgcttcgaat aacttcggcg taaaatcgag
     1081 atacttttct gaagaccaca actgctcttc tggccacagg cctttggttg ccggttcata
     1141 cgccagtcct ttgcccttcg ccaggccata agtggtttgc atacccggta caccgcactg
     1201 cacacgaatc gccttaaagc ccatctgctg gtgctttgca tagtcttcaa ggacttcatc
     1261 aatcgtacgc ccggtggtgt ggcaataaac catcacgcct tcacgagaag caccaccaag
     1321 cagttgatac aatggcatat ttgccgcttt agctttgata tcccacaacg ccatatcaat
     1381 ggcagaaatg gctgacatcg tgacggggcc acgtcgccag taagcacctt tataaaagaa
     1441 ttgccagata tcttcgatac ggctggcatc gcggccaatt agttgtggac aaagatgatc
     1501 tttcagataa gaagcgaccg acaattcgcg accatttaac gtggcatctc ctaatccaca
     1561 aagtccgctt tcagtggtta ttttaagcgt aacgaaatta cgccctgggc aagtaacaaa
     1621 aacatctgca gcaataattt tcattattgc aatctcctgt gttgtaaata gacgtttcaa
     1681 acctaatttt acgcctacta ccatacaaga gagaaaaagc gattattcaa ccgtgatcac
     1741 attaaaaaca aaaatttcca ccgaggagaa aaaataccct caaaaaccac ctgaataatt
     1801 aaccattatt gttaaaacaa cacgttaaat aatcacataa caaactccat taatcattga
     1861 aaatgagcac agaaatagaa attaattata ttcaattaat taaaatgtgt attgcttgat
     1921 gtatttaaaa atattatttt catggtgaga gtggaggtaa agaagacaca ataataaaaa
     1981 ggaatgtgac aaataatggt tctggttggg cgttaaattt aaggttacca gaa
//
LOCUS       DAJTQY010000223         2025 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.229, whole genome shotgun sequence.
ACCESSION   DAJTQY010000223 DAJTQY010000000
VERSION     DAJTQY010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.229"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            6..224
                     /locus_tag="OO349_004058"
     CDS             6..224
                     /locus_tag="OO349_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HCR5868923.1"
                     /translation="MVTLFRKKYPRKSRTTEFLFLILFIVLMTPISPQIFVWIIGKII
                     EPVIELYNDVVWASFNTLHNKINPYKES"
     gene            226..591
                     /locus_tag="OO349_004059"
     CDS             226..591
                     /locus_tag="OO349_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR5868924.1"
                     /translation="MALTKKQRAELRMKFGGRCAYCGCELGEKWHADHVKPVIRFDGN
                     MLHQERDDISNMVPECHPCNLHKHCSSLEDYRRIISDGRREFLASGKGKALVRMGLVE
                     MKSDPVVFWFEKYQEGATA"
     gene            588..1253
                     /locus_tag="OO349_004060"
     CDS             588..1253
                     /locus_tag="OO349_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5868925.1"
                     /translation="MTTITKERLQWLANISGRDDIDDIDGGEIRELALIALASLEAEP
                     VAECIVEDGGMCIDGFGEYVGHSLPDGMHELYAAPRMRQPDGWKAVAVAWKVTFTQVD
                     QESNTFTAIYFYKAEVERWVRLHKACDFRADITPLYAAPAVPVAVNDDMAYAFHHALS
                     DSSLGSDEIEEIKTGLRAAFANVTIQPEPIVPDEIGPNDSNTFDYVDGWNACRAAMLK
                     GDK"
     gene            1253..1618
                     /locus_tag="OO349_004061"
     CDS             1253..1618
                     /locus_tag="OO349_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HCR5868926.1"
                     /translation="MINRTKLEHILEYARQQKCIGQLCKIPPGDMVEIVEMAMRKAGN
                     SPVTLAGWISCSDAVPAEYCDVILLDDLGNVFPGSWDKVFCPTRGGNKMAFVDKDGVE
                     VESSTHWMPLPEPPQEVNQ"
BASE COUNT          554 a          420 c          538 g          513 t
ORIGIN      
        1 aatgaatggt tacattattt agaaaaaaat atccgcgaaa gagtagaaca acagaattcc
       61 tgtttctcat tctgtttatc gtgttgatga caccgatatc cccgcaaatt tttgtctgga
      121 taatcggaaa aataattgag ccagttattg aattgtataa cgacgtggta tgggcgtcat
      181 tcaacacact gcacaataaa attaatccgt ataaggaaag ctgatatggc actgacgaaa
      241 aaacaacgtg cagaactgcg catgaagttc ggtggtcgct gcgcttattg cggctgcgaa
      301 cttggcgaaa agtggcatgc agaccatgta aaaccggtca ttcgttttga tggaaatatg
      361 cttcaccagg aacgtgacga tatatccaac atggtaccag aatgccaccc atgcaatctg
      421 cacaagcatt gcagtagcct ggaagattat cgacgaatta tcagtgatgg tcgtcgtgaa
      481 ttccttgcgt ccgggaaagg caaagcgctg gttcgtatgg gattggttga aatgaaatct
      541 gacccggttg tgttctggtt tgaaaaatat caagaagggg ctacggcatg actactatta
      601 ccaaagagcg actgcaatgg ctggctaaca tttctggccg cgatgatatt gacgatatag
      661 acggcggtga aattcgtgaa ctggcgctta tcgctctggc atcactggaa gcagagcctg
      721 tagcggaatg cattgttgaa gatgggggca tgtgtattga cgggttcggt gagtatgtgg
      781 gtcactcgct gcctgatgga atgcatgagc tttatgctgc cccgcgaatg cgacaacctg
      841 atggatggaa agccgtagct gtagcgtgga aggtgacgtt tactcaagtt gaccaggaat
      901 ctaatacgtt cactgctata tatttttaca aagcggaagt tgagcgttgg gtacgactgc
      961 ataaagcatg tgattttcgg gcagatataa caccgcttta cgcagcgccg gcagtgccgg
     1021 ttgcagtaaa cgacgacatg gcttacgcat tccatcacgc actgtcagat tcatcgcttg
     1081 gctctgatga aatcgaagaa attaaaaccg gtttgcgtgc tgcctttgcc aacgtcacta
     1141 tccaaccaga gccgatagtg ccggatgaaa tcgggccaaa cgatagtaat acgtttgatt
     1201 atgttgatgg ctggaacgcc tgccgcgctg ccatgcttaa gggagataaa taatgattaa
     1261 tcgaaccaaa ctggagcaca ttctcgaata tgccaggcag cagaaatgta ttgggcaact
     1321 ttgtaaaatt ccaccaggag atatggttga aatcgtggaa atggccatgc gtaaggctgg
     1381 caactctccg gtaactctgg ctggctggat aagctgtagt gatgcagttc ctgctgaata
     1441 ttgcgatgtg attcttctcg atgatctcgg gaatgtattc cccggttcct gggataaggt
     1501 tttttgcccc actcgtggcg ggaataagat ggcttttgtg gacaaagacg gcgttgaagt
     1561 agagagctca actcactgga tgccgctacc ggaaccaccg caggaggtga atcaatgacc
     1621 tggcctgagg cattcaccac ggtaggaatt gcgatggcgg tggcgctggt ggtgtattcg
     1681 atttgccgtt ggggttaaca aacaaaaacc ccggattgat ggtccggggt ttttgaagga
     1741 aacaaaacag aaacagcaat tgccgttacc tgttgttacc atggcaagta aacgtatctc
     1801 aggcgagcgc attgcgccgt tctgacgcag ataaattagc ctggatagaa ggtgctggca
     1861 ataaaaaata gcgttttctt atcggtatcg gtaagattgc tgcgggtgct tgaggctgtc
     1921 tgcctcgggc atgccactgt aaggcagaca gagaaaagcc ccagttaaca ttacgcgtcc
     1981 tgcaagacgc ttaacattaa tctgaggccc aatctatgtc tcaca
//
LOCUS       DAJTQY010000224         1953 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.230, whole genome shotgun sequence.
ACCESSION   DAJTQY010000224 DAJTQY010000000
VERSION     DAJTQY010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1953
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.230"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            41..1438
                     /gene="ccp"
                     /locus_tag="OO349_004062"
     CDS             41..1438
                     /gene="ccp"
                     /locus_tag="OO349_004062"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HCR5868927.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHTLNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            1649..>1953
                     /locus_tag="OO349_004063"
     CDS             1649..>1953
                     /locus_tag="OO349_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR5868928.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCV"
BASE COUNT          539 a          403 c          493 g          518 t
ORIGIN      
        1 tttctgggtc acaataactc accaccgtga gggaagaata atgaaaatgg tctcacgtat
       61 taccgcgatc ggcctggctg gcgtcgcgat ttgctattta gggttatctg gttatgtgtg
      121 gtaccacgat aataaacgca gtaaacaggc cgatgttcag gcatctgctg tcagtgaaaa
      181 taataaggtt ttaggctttc tccgcgaaaa aggatgcgac tattgccaca cgccttcggc
      241 agaattaccc gcctattatt atattcctgg cgcgaaacag ttgatggatt acgacattaa
      301 gcttggatat aaatctttta acctcgaggc cgtgcgtgcg gcactgctgg ctgataaacc
      361 cgtttcgcaa agcgatctga ataagattga atgggtgatg cagtatgaaa ctatgccacc
      421 aacgcgttat accgcgctac actgggcggg taaggtgagt gatgaagagc gggcggaaat
      481 actggcctgg attgcaaaac agcgcgcgga atattacgcc agcaatgata ctgctccgga
      541 gcatcgcaat gaaccggtgc agcccatccc gcaaaaactg cctaccgatg cgcaaaaagt
      601 ggcgttgggc tttgcgctgt atcacgatcc ccgtttatcg gctgatagca ccatttcatg
      661 cgctcattgc catacgttga atgcgggggg cgtcgatggc agaaaaacat cgattggtgt
      721 tggtggcgca gttgggccga ttaatgcgcc gacggtattt aactcagtat ttaacgttga
      781 gcagttctgg gatggtcgtg cggcaacatt gcaggatcag gcaggtggac cgccgttgaa
      841 cccgattgaa atggcgtcga aatcctggga cgaaattatt gctaagctgg aaaaagatcc
      901 gcagcttaaa gcgcagttcc tcgaagttta tccgcaaggt ttcagtggcg aaaatattac
      961 tgatgccatt gctgaatttg agaaaacatt aattacgccg gattccccat ttgataaatg
     1021 gttgcgtgga gatgaaaatg cgctgacggc gcaacagaaa aaaggctatc aattatttaa
     1081 agataataaa tgtgcaactt gtcatggtgg tattattctc ggcggacgtt cctttgaacc
     1141 gttggggctg aaaaaagact ttaactttgg ggaaattacg gcggcggata ttggtcgtat
     1201 gaatgtgact aaagaagagc gtgataaatt gcgtcagaaa gtacccggtt tacgtaacgt
     1261 tgctttaacg gcaccgtact tccatcgcgg tgacgtgccg acgctggacg gggcggtgaa
     1321 actgatgctg cgctatcagg taggcaaaga gctgccgcag gaggatgtgg atgatatcgt
     1381 agctttcctg cacagtctga acggggtgta cacgccgtat atgcaggata aacaataatt
     1441 aatttgatcg cccgaacagg aatgtttggg cgatttttat tacgataata aagtctgttt
     1501 ttaatattat catgttaaat gtttatatta taaaaagtcg tttttctgct taggattttg
     1561 ttatttaaat taagcctgta atgccttgct tccattgcgg ataaatccta cttttttatt
     1621 gccttcaaat aaatttaagg agttcgaaat ggaccagaag ctgttaacgg atttccgctc
     1681 agaactactc gattcacgtt ttggcgcaaa ggccatttct actatcgcgg agtcaaaacg
     1741 atttccgctg cacgaaatgc gcgatgatgt cgcattccag attatcaatg atgaattata
     1801 tcttgatggc aacgctcgtc agaacctagc cactttctgc cagacctggg acgacgaaaa
     1861 cgtccataaa ttgatggatt tgtcgatcaa taaaaactgg atcgacaaag aagaatatcc
     1921 gcaatccgca gccatcgacc tgcgttgcgt aaa
//
LOCUS       DAJTQY010000225         1913 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.231, whole genome shotgun sequence.
ACCESSION   DAJTQY010000225 DAJTQY010000000
VERSION     DAJTQY010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.231"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(53..1702)
                     /locus_tag="OO349_004064"
     CDS             complement(53..1702)
                     /locus_tag="OO349_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HCR5868929.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALTVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
BASE COUNT          451 a          506 c          492 g          464 t
ORIGIN      
        1 gaagaggata attttacagc cagcaggaag ttagcggctg gctgtaaaaa tattatggtt
       61 cgccgtacaa accaattaaa cggcgtacca caccgttagt ccagccaaac ccatcctgca
      121 acggatactc gccaccgccg ccttcgcggg gaacaccatc ggcaatatgg tatttctcga
      181 tcattttgtg ctgttccaga tagaactgat tcaccgtttt cagccagctg cgcgcgattt
      241 catcacccag aaggtcatcg ccatacattt taaatccctg aattgccatc cattgtaacg
      301 gtgcccagcc gttgggttta tcccactgtt caccggtttc gtactcgctt gccaaaatcc
      361 cgccaggtgt cagtaaccgg ctgcgtacgg cgtttgccag acgatcggcc tgttcatggt
      421 tcgccatgcc gacatagagc ggcacaatgg cggcagcgga aaacagcgcc agttgttcgc
      481 gtcgccagtc gtaatcgcgg tagatgccgt tttcatcatc ccagaggtaa cggtttaccg
      541 catcgcgacg ggcactggcc ttctggcgga acagcgcttc tgtctctttc tcgcctttca
      601 gcgccgagat gttggcgatg gcgctctcca gtttaaacag gaaggcattc agatcgatgg
      661 ggatgaactg ggtggtacga atgctcgcca gacgaccagt atcacgcagc caacgggaag
      721 agtaatccca accggaggcc gctcccgcgc gtaaatcgcg gtacacctcg ttgggcgggc
      781 gaccagaatg tttcgcggtt tcaacgtcct caagccagga ttcgtcacgc ggcgtgtcgc
      841 gatcgtccca gtaacggttg agcagcgatc cgtccggcat ccgcacaaca tggcgatagg
      901 cctgattagg gatcaacgat tctgcaccgt ccatccagaa ggcatattcc attttaaggt
      961 ggtcgagata gcggcgcgca ccgcgtacac catcttcttc aaacaactcc accgtcagcg
     1021 caaaaaccgg tggttgcgag cggctcaaat aataggtgcg gttgccgttg gggatgtgac
     1081 cgtagttttc gatcatccag gcgaagttat cggccatgca tttcagcaaa tcttcccgac
     1141 cactttccgc cagtcccagc atggtgaaat aggaatccca atagtaggtt tcgctaaaac
     1201 ggccgcccgg gacaatatat gactgcggta gcgccagcag agaagaccac ggaatgtgat
     1261 cctgtggttc gcgggttagc accggccaca gctggtcgat atgctctttc agggaatttt
     1321 gcgggtccga tacatactcg ctggagtaga cctccggcag ccagaagtga ttttcaacaa
     1381 acttgcgcaa gtcaaaatca cgatgacggc gcactttacg gtagcggatt aaaatatcca
     1441 gcgggtccat tttgggtgcg cagtcgggaa aggttttact gtcggggaaa attttcgccg
     1501 actgaacatg ctcgaacagt tcgagatagc gatcggcagg cgtcagcgca tcagaggcgg
     1561 gcagcccttc aatcatctcg ggttccggtt ctgcctcgat catctcatcc agttttaatt
     1621 catacgggtc cagttcatag cagagatcga cctcgatcat cagttcgtct ggattagggt
     1681 tttgaatttt ctgattgagc ataaggatcc aacctccgaa attcgtcgct aattatcggg
     1741 cttttcttta agggtagtca ttcgctccgc ctggtgggcg acaaagcgtg cggtggatca
     1801 cgtttattaa cttgtcaaaa aatataacgc actaatatca atggaattta taaaaatacg
     1861 gatttggtgt tgtttttcat tttcagaaca ttcaatcctg ctctcgccgc aat
//
LOCUS       DAJTQY010000226         1882 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.232, whole genome shotgun sequence.
ACCESSION   DAJTQY010000226 DAJTQY010000000
VERSION     DAJTQY010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1882
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.232"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            51..272
                     /gene="kilR"
                     /locus_tag="OO349_004065"
     CDS             51..272
                     /gene="kilR"
                     /locus_tag="OO349_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="killing protein KilR"
                     /protein_id="HCR5868930.1"
                     /translation="MIAHHFGTDEIPRQCVTPGDYVLHEGRTYIASANNIKKRKLYIR
                     SLTTKTCISDCMIKVFLGRDGLPVKAESW"
     gene            266..442
                     /locus_tag="OO349_004066"
     CDS             266..442
                     /locus_tag="OO349_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5868931.1"
                     /translation="MVMTKKIKCAYHLCNKEIEESKIITRPLHFMRGVIPTTEMKKYC
                     SEICAEKDQMAHEL"
     gene            517..792
                     /locus_tag="OO349_004067"
     CDS             517..792
                     /locus_tag="OO349_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5868932.1"
                     /translation="MITSYEATVVTTDDIVHEVNLEGKRIGYVIKTENKETPFTVVDI
                     DGPSGNVKTLNDGVKKMCLVHIGKNLPAEKKAEFLATLIAMKLKGEI"
     gene            892..>1882
                     /locus_tag="OO349_004068"
     CDS             892..>1882
                     /locus_tag="OO349_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HCR5868933.1"
                     /translation="MNTDKQVYPLYYEAKNDKVRKRLGIKGGFYWAEAKKLSIAISRG
                     AVAIDDAGYDEDDFKKPVRVNLPVVDDLPPEGVFDTEFCNRYEKGGEDGITMVFIASS
                     PSVQDKPASTDNTNVNGEDMTEIEENMLLPVSGQELPIRWLAQHGSEKPVTHVSRDEL
                     QALHIARDEELPAVTALAVSHKTSLLDPLEIRDLHKLVRDTDRVFPNPGNSSLGLMTA
                     FFEAYMDADYTDRGLLTKEWMKGNRVSRITRTASGANAGGGNLTDRGEGFVHDLTSLA
                     RDVATGVLARSMDVDIYNLHPAHAKRVEEIIAENKPPFSVFRDKFITMPGGMDY"
BASE COUNT          580 a          419 c          420 g          463 t
ORIGIN      
        1 ataactttcc tgagatttcc tttgtaagac tgtttattaa ggaggcttcc atgattgctc
       61 atcacttcgg aactgatgaa ataccacgtc agtgtgtgac tcctggcgat tatgttcttc
      121 atgaaggtcg gacatatatc gcctcggcaa acaatattaa aaagcgaaaa ctttatattc
      181 gtagcctgac tacaaaaaca tgcatttctg actgcatgat taaagtcttc ctcggtcgtg
      241 atggtttacc tgtaaaggcg gagtcatggt aatgactaag aaaataaaat gtgcttatca
      301 cctttgcaat aaagaaattg aagaaagcaa aatcattaca agaccacttc atttcatgcg
      361 tggagttata ccaacgacgg aaatgaaaaa atattgtagt gaaatctgtg ccgaaaaaga
      421 ccagatggca cacgaacttt aattaactga ctatccgaaa ctgaatttat gccagcaatg
      481 gcagggattc gctcaacctt aattaaggag aaaaacatga ttaccagtta tgaagccact
      541 gttgtaacta ctgatgacat tgttcacgag gttaatctgg aaggaaagcg tattggctac
      601 gtgattaaga cagaaaataa agaaacccca ttcactgtgg ttgatatcga cggtccatca
      661 ggcaacgtta aaacacttaa cgatggtgta aaaaaaatgt gtctggtgca cataggaaag
      721 aatctgcccg cagaaaaaaa agccgaattt ctggcaactc tgattgcaat gaaattaaaa
      781 ggtgaaatct gaaaaaaaga aagcctgcac actgtgcagg cctgaatgaa gaacctggga
      841 catttattca tcactcgcag taattttaat ctgagttgag gttaaaaaac aatgaacacc
      901 gataaacaag tttacccact gtattacgaa gcaaaaaatg acaaagtaag aaaacgtctc
      961 ggtattaaag gcggttttta ctgggctgaa gcgaaaaaat tatccattgc catctcccgt
     1021 ggtgctgttg cgattgacga tgcaggctac gatgaagatg actttaaaaa acctgttcgc
     1081 gtcaatttgc ccgttgttga tgacctcccg ccagaaggcg tatttgatac ggaattctgc
     1141 aaccgttacg aaaaaggcgg ggaagatggc atcacaatgg tatttatcgc atcttctccc
     1201 tctgttcagg acaaaccggc cagcactgac aataccaacg tcaacggcga agacatgaca
     1261 gaaattgagg agaacatgct cctgccagtt tccggtcagg aactgcccat tcgctggctt
     1321 gcgcaacacg gcagcgaaaa accagtaacg cacgtttcac gcgacgaact ccaggcatta
     1381 cacattgctc gggatgaaga actaccagct gttactgccc tggctgtttc ccacaaaacc
     1441 agcctgctcg atccgctgga aattcgcgat ctccacaaac tggtgcgtga tactgacaga
     1501 gttttcccta atccaggcaa ttcaagcctg gggctgatga ctgctttttt cgaagcatac
     1561 atggacgcag actacaccga tcgcggtctg ctgacaaaag agtggatgaa aggaaatcgt
     1621 gtttcacgca tcacgcgcac ggcttccggc gctaatgctg gcggcgggaa cctcaccgat
     1681 cgcggcgaag gtttcgttca cgatctgacg tcactggcgc gcgacgtagc cactggcgta
     1741 ctggctcgtt caatggacgt ggacatttat aaccttcatc cggcacacgc taaacgtgtc
     1801 gaggaaatta tcgctgaaaa taaaccgccc ttttctgttt tccgcgacaa attcatcacc
     1861 atgcctggcg ggatggatta tt
//
LOCUS       DAJTQY010000227         1827 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.233, whole genome shotgun sequence.
ACCESSION   DAJTQY010000227 DAJTQY010000000
VERSION     DAJTQY010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1827
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.233"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(1..337)
                     /locus_tag="OO349_004069"
                     /pseudo
     CDS             complement(1..337)
                     /locus_tag="OO349_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            complement(337..1257)
                     /locus_tag="OO349_004070"
     CDS             complement(337..1257)
                     /locus_tag="OO349_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR5868934.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYPAIVVSHPGG
                     GVKEQTAGTYAEKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDVSAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPHIEAGSNARTSDISSEEYAIMPLAPMKEADAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            1395..>1827
                     /gene="pgrR"
                     /locus_tag="OO349_004071"
     CDS             1395..>1827
                     /gene="pgrR"
                     /locus_tag="OO349_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HCR5868935.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMVQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPILHDIDSALTSLSDLQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAG"
BASE COUNT          456 a          442 c          424 g          505 t
ORIGIN      
        1 ccagcacgcc aatgcgttca gcatcaacat atggcagagt tactgcataa tcgataacac
       61 ggctaatatc ctctacgcgc tgtgtcggat cttctatcca acgaggctac ccaccagatt
      121 ctccctggaa actggcgtca taagcgagaa cgacatagcc tttttcagcc aatgccttgc
      181 catagatatt gccagacgtc tgctccttac aactgccaaa aggatggacg ctgataatcg
      241 ccggatactg ttttgactca tcaaaatttg gtggaaactg aatattggca gcaatatgcc
      301 aggccatatc actattgcgg atttgaatat gtttcattac agagtttcct caaagaacgg
      361 agctaatact gaaattgctt cagccacata ggctttgccg tcataaagat ccatatggtt
      421 tgcgccttca acaatgtgat agcgtttatc ctggcttgat gcccgatcgt acaggtcgtc
      481 actcatccat ttgctccctg cctggctgcc cgccacaatc tgcatcggct gagtcaggta
      541 cacttccgcc atatggtaag catcataggt aataatctgg ttaaggctgc gcaaagtagc
      601 gtaacccggt gctgttggat actgcgcacg aggtgtgtgg taatactccc aggcctgacg
      661 cagttcttcg ttcggcgcat cggcctcctt cattggagcc agtggcataa tggcgtattc
      721 ttcgctgcta atatcgctgg ttcgggcgtt tgagccagct tcaatgtgtg gcagagcatc
      781 aatagatttc acattgtttt cccaaccatt gcggaacatc gaaccaatat tgaccgcact
      841 aacggtaccg atggccttga tgcggcgatc ctgaattgca gcattggctg tatatcctgc
      901 accggcacaa attcccatcg caccaattcg ggtattgtcg acataagaaa gcgttgtcag
      961 gtaatcaatc acggcactga cgtcttcagt acgaatgtat gggttttcta actgacgcgg
     1021 ctcgccgcca ctttcacctt gataagatgc gtcataagca atagtgacaa atcccttttc
     1081 cgccagcttt tcggcatagg ttccggccgt ttgctcttta acgcccccac ctgggtgaga
     1141 taccacaatt gccggatact gacgggtttc atcaaatttt ggagggaaat agatcactgc
     1201 agacaaagag atggttggat tattgctgtt agtgaagctg actttattat tcatcattca
     1261 gttcctcatg gagttgtcgg ttcgttttga cggttggtga tatcactata gatattgatc
     1321 attaagttga ttagacccta aatcatgatt agacctatca tttaaatgat taatagattg
     1381 tatggagata ctgaatgaag cgtgaagaaa tcgctgatct gatggcgttt gtcgtcgttg
     1441 cagaggagcg tagcttcact cgcgcagcag cccgcctgag catggtgcag tcagctttaa
     1501 gccagatagt gcgccgtata gaagaacgat tgggattgcg gcttctgacg cgaaccacgc
     1561 gcagcgttgt acccactgaa gcgggcgagc atcttttgtc tgttcttggc cctatattgc
     1621 atgacataga ttctgccctg acatccctga gcgatctgca gaaccgccca tccgggacaa
     1681 tacgtattac tactgtagaa catgcagcaa aaacgatatt gttaccagcc atgcgcacat
     1741 tcctgaaatc gcatcctgaa attgatattc agcttaccat tgattatggt ttgaccgatg
     1801 tcgtttctga acgttttgat gcaggcg
//
LOCUS       DAJTQY010000228         1810 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.234, whole genome shotgun sequence.
ACCESSION   DAJTQY010000228 DAJTQY010000000
VERSION     DAJTQY010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1810
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.234"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            258..605
                     /gene="grxD"
                     /locus_tag="OO349_004072"
     CDS             258..605
                     /gene="grxD"
                     /locus_tag="OO349_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HCR5868936.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            complement(644..1144)
                     /gene="tnpA"
                     /locus_tag="OO349_004073"
     CDS             complement(644..1144)
                     /gene="tnpA"
                     /locus_tag="OO349_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HCR5868937.1"
                     /translation="MILWSVKKETDIRRGRHCVFLMHVHLVFVTKYRRQIFDHDTTEK
                     LRTYFSNVCADFAAELVEMDGEPDHVHLLINYPPKLAISSLVNSLKGISNRLLRRDRP
                     DIAVRYYYKGVLWSPGYFASSCEGAPISVIRQYIEQQQTPGQVENRALYPRPEGRGFT
                     THRIKD"
     gene            1152..>1810
                     /locus_tag="OO349_004074"
     CDS             1152..>1810
                     /locus_tag="OO349_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5868938.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFQKRAAFPR
                     FKKRGQNAAFRYPQDVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGTWY
                     ISIQTESEVSTPVHPSASMIGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQL
                     SR"
BASE COUNT          510 a          417 c          450 g          433 t
ORIGIN      
        1 aagagaaagt taatagcttt tattgttaaa aaatacgttt cgttggcgag cgcaaaaaaa
       61 tgcagcagtg aaagctaaaa atggttataa atacgccagt ttgccgcgca tcagagaaga
      121 aacgaatatt tgaaatgcaa caaatagagc aaagggataa aatagcaaaa gcgtgatgaa
      181 aatgttattt tccgatgccg cactgacccg ctacaatgtc agattactgc cgtaaagaag
      241 tttgaggaag caagacaatg agcaccacta tcgaaaaaat ccaacgccag attgctgaaa
      301 acccgatcct gctgtacatg aaaggttcac cgaaactgcc gagctgcggt ttctctgccc
      361 aggcagtcca ggcgcttgcc gcatgtggcg aacgttttgc ctatgttgat attctgcaga
      421 atccggacat tcgtgcggaa ctgccgaaat atgctaactg gccgaccttc ccgcaactgt
      481 gggttgacgg cgagctggtc ggcggttgtg atatcgtgat cgaaatgtat cagcgtggcg
      541 aactgcagca gctgatcaaa gaaactgccg ctaaatacaa gtctgaagag ccggacgcgg
      601 aataatccgt cctgcaatac tgacggatgc gaacgggcat ccgtcagtcc tttatccggt
      661 gcgtcgtaaa acctcgtcct tcagggcggg gatataaggc gcggttttcc acctgaccag
      721 gtgtttgctg ttgttcaatg tactggcgga tgacggatat tggcgcacct tcgcagctac
      781 tggcaaaata gccaggactc cacaaaacgc ctttgtagta atacctgact gcaatatctg
      841 gtcgatctcg tcgcagtaac ctattcgata tgcctttgag gctgtttacc agactggata
      901 tcgccagttt gggaggatag ttaattaaca aatggacgtg atctggttcg ccatccattt
      961 caaccagttc agctgcaaaa tcagcacata catttgaaaa gtaagtgcgt agtttttctg
     1021 tcgtgtcatg gtcaaaaatc tggcgtcggt atttggtgac aaagaccagg tgaacatgca
     1081 tcaggaaaac acaatgcctt ccacgccgga tatcggtttc ttttttcaca gaccaaagta
     1141 tcatttgacc tgtgaaacga ttacaggcat ttaaattcca gttaagacca ggtggtcaac
     1201 aggagcgtga aatgaggcgc ttcgcaggcg catgtcgttt cgttttcaat cgtgctctgg
     1261 cgcttcagaa tgagaatcat gaggccggta ataaatacat cccttacggg aaaatggctt
     1321 cctggctggt tgagtggaaa aacgccactg aaacgcaatg gcttaaagat tcgccctcac
     1381 agccattgca acagtcactg aaagaccttg agcgggctta caaaaacttc ttccagaagc
     1441 gggctgcttt tccccgattc aaaaagcggg gacagaatgc tgcattccgc tacccgcagg
     1501 acgttaagct cgatcaggaa aacagccgta tttttctgcc gaaacttggc tggatgcgct
     1561 accggaatag ccgtcaggtc acgggagttg tgaaaaatgt cactgtcagc cagtcctgcg
     1621 gtacgtggta catcagtatt cagacagaaa gtgaagtatc aactccggtt cacccttcag
     1681 catcaatgat cgggctggat gctggcgtgg ctaaactcgc cacgctgtca gatggcacag
     1741 tctttgagcc tgtaaacagt tttcaaaaaa accagaagac gctggcgaga cttcagcgac
     1801 agttaagccg
//
LOCUS       DAJTQY010000229         1805 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.235, whole genome shotgun sequence.
ACCESSION   DAJTQY010000229 DAJTQY010000000
VERSION     DAJTQY010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1805
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.235"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            173..1198
                     /locus_tag="OO349_004075"
     CDS             173..1198
                     /locus_tag="OO349_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5868939.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPRFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            1266..1805
                     /locus_tag="OO349_004076"
                     /pseudo
     CDS             1266..1805
                     /locus_tag="OO349_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc.;
                     frameshifted; incomplete; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
BASE COUNT          421 a          420 c          461 g          503 t
ORIGIN      
        1 caccattgcg gggcgctgtt tttattattt cctgtctaca ctcagcttat tgcgacgcga
       61 aatcgtgcgc aaaagctggc actacttttg cttataagag gatggccaca gacaggtaaa
      121 agacgtttcc caaacgtcct ataacgataa ttttcgcctc acaggaagca ttatgaaaaa
      181 gatgatgata gccacactgg ctgccgccag cgtgctgctt gccgttgcaa atcaggcgca
      241 tgctggcgcg acgcttgatg ccgttcagaa aaaaggtttt gtgcaatgcg ggatcagtga
      301 tggattacct aggttctctt atgccgatgc agacggtaag ttttcaggta ttgatgttga
      361 tgtttgtcgt ggtgttgccg ctgctgtatt tggtgacgac acgaaagtga aatatacccc
      421 actcacagca aaagaacgct tcaccgcttt acagtcaggg gaagtggatt tgctctcccg
      481 taatacgacc tggacttcat ctcgcgatgc cgggatggga atggcattta ccggcgtcac
      541 ttattacgac ggcattggct tcctgacgca cgataaagcg gggctaaaaa gcgcgaaaga
      601 actggatggc gctaccgtct gtattcaggc gggtactgat accgaactca acgtcgccga
      661 ctatttcaag gcaaacaata tgaagtacac accagtgact ttcgatcgct ctgacgaatc
      721 agcgaaggca ctggaatctg gtcgctgcga tacgctagct tcggatcagt cgcaactgta
      781 tgccttgcgc atcaaattaa gcaacccggc tgaatggatt gtcttaccgg aggttatctc
      841 taaagaaccg cttgggccgg tagttcgtcg tggcgatgat gaatggttct cgattgtacg
      901 ctggacgctt ttcgccatgc taaatgctga agagatgggc atcaattccc agaacgtcga
      961 tgaaaaagcg gctaatccag caacgcctga tatggcacat ctgttgggta aagaaggcga
     1021 ttacggcaag gatctgaagt tggataataa atgggcctat aacatcatca aacaggtggg
     1081 taactactcg gaaatttttg agcgtaacgt aggttcagaa agcccgctga aaattaaacg
     1141 tgggcaaaat aatctctgga ataacggcgg tattcagtac gcgccgcccg tgcgttaagt
     1201 cggtgtgagg taacgagcgt cgctacggcg gcgctctctt cagcgttatg ctttccgagg
     1261 tttctatgtc tcatcgccgc tcaaccgtta aaggctcact ctcctttgcc aaccctacgg
     1321 ttcgtgcctg gttattccag atccttgccg ttgttgctgt tgtcggcatt gttggttggt
     1381 tatttcaaaa cactgtaacg aatctcaata atcgtggcat tacttcaggt tttgcctttc
     1441 tggatcgcgg cgctggcttc ggtattgtcc agcatttgat cgattaccag cagggcgaca
     1501 cctacggacg cgtttttatt gtcggcttac tcaatacgct actggtttct gcattgtgta
     1561 ttgtgttcgc ttctgttttg ggcttcttta tcggtctggc gagactttcg gataactggc
     1621 tgctacgaaa gctttccaca atttatattg agatcttccg taatattgca aatcttcttc
     1681 tggtactttg ccgtgttgcg caatttgccc ggaccacgcc aagctgtgag cgcgtttgat
     1741 ctggcctttt tgagcaatcg tgggctttat attccgtcac cgcagctggg agacggattt
     1801 attgc
//
LOCUS       DAJTQY010000230         1771 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.236, whole genome shotgun sequence.
ACCESSION   DAJTQY010000230 DAJTQY010000000
VERSION     DAJTQY010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1771
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.236"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            42..221
                     /locus_tag="OO349_004077"
     CDS             42..221
                     /locus_tag="OO349_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309392.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5868940.1"
                     /translation="MDLSPEDAKFLRELQHRMLREAIIEQQTGMSAIDPLRYKRASSP
                     LVDDNSNADKTRPPH"
     gene            273..467
                     /locus_tag="OO349_004078"
     CDS             273..467
                     /locus_tag="OO349_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5868941.1"
                     /translation="MNENKIKRLEQLLQARQREFATKWGRAALPHERLTYFPLEDLIK
                     NNGKKIRSDKKHFLECNEDK"
     gene            complement(1266..1602)
                     /locus_tag="OO349_004079"
                     /pseudo
     CDS             complement(1266..1602)
                     /locus_tag="OO349_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          544 a          390 c          367 g          470 t
ORIGIN      
        1 agcgtaaggc actacagcgc attgctttac gagtaataac aatggattta tcaccagaag
       61 acgcaaagtt tttacgagaa ctgcaacacc gtatgctcag agaagccatt atcgaacagc
      121 aaacgggcat gagcgccata gacccattgc gatacaaacg ggcctcatct cctttagttg
      181 acgacaattc caatgcggat aaaacacgtc caccacactg attacatttt tcattagcac
      241 agtggtgtga tgcagcatac gaggaaaacc ggatgaatga gaacaaaata aaaaggctgg
      301 agcaactgtt acaggcacgc caacgggagt ttgccactaa atggggacgt gccgcgctgc
      361 cacatgaacg cctaacctat tttccacttg aagacctcat taaaaataat ggcaaaaaaa
      421 tcaggtctga taagaaacac ttcttagaat gcaatgaaga caaataacaa caatctgcat
      481 attgccacca accacagatg tggcatcgtt atcagcgcac ttgattttct ttatattcac
      541 tgacgagctt gccggggcac ctttgtttgc cggtcattct ggtatcatgc agatatttac
      601 ggcgtacaag cccactagca tgacattgta ataaggcctg agcctaacgc tttaatgtgt
      661 gtcatacact cagtggcgta atcgttgaca atatggtgca tgtaatcgta agcgtccagc
      721 gaaatccatc tggtcataat ctgaagcatc cgacaaagtg gtgtccacca aataagtgat
      781 ctgcgcccaa tatcctgagc cggaactttg acaaaggcca acgaaaaatg ggagcggatg
      841 ttacagagtt ctcggtacag gggaaaagtg gtacctgtcg ccagttctcc gatctgttta
      901 accgggaaat tatctcctac agcttgtcag aaaggtcggt gatggagcat cgttaatacg
      961 atgctatacg atgcattcac agtgcccggc cagaggatgc cccgtcgctg catatggatc
     1021 agggttggca atatcgaatc gcaggctata ggcaaagtta aagccccatg gagtagcaca
     1081 aaatatgccg cacaaaggaa acggtctgaa taacgcagtg atgaagaact tcttcagcac
     1141 actgctaaaa cgcatagtga tcgagcgctg attctggcga acaactgaac taatacatca
     1201 gaatatgcat tatgttaaat aaatatgaaa agatggttta aataccccgt tacttttgac
     1261 ttacactata cggtatcgca tcgtttaata ttcgcaccgg ccagattttt atttctatta
     1321 gttgtcacaa tactgaatgc gtacgaccac agtattctgg ctcctgtgtg gttatgcttt
     1381 aattctgcgt tccgggcaga taagcagttg cttgcaggaa tccttcttgt gttaatgtca
     1441 gttccccttt ttaccagtgc tgatttccac attccgtcca acagagctta tagcctttcc
     1501 ctggattata gcattgtccg gctgaagttc tttttgaata ataatagaag cactgctggc
     1561 agatccagtc cgtttttcat aacccactgt actgataacc ataatctaat cagtagaaat
     1621 tgagtcgaaa ataagcacta ctccatacag gataattaga ggtcagtttg attattcaca
     1681 attcatcatc agcattttct atttctgacg aaatcaacat gaaaataacc atatatgata
     1741 attattataa taacggcttt aattggaata c
//
LOCUS       DAJTQY010000231         1766 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.237, whole genome shotgun sequence.
ACCESSION   DAJTQY010000231 DAJTQY010000000
VERSION     DAJTQY010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1766
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.237"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            <1..107
                     /locus_tag="OO349_004080"
     CDS             <1..107
                     /locus_tag="OO349_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HCR5868942.1"
                     /translation="QTHLDTALETFWQALHRRDVQVPDLLSLRGNAHA"
     gene            100..1059
                     /gene="ssuA"
                     /locus_tag="OO349_004081"
     CDS             100..1059
                     /gene="ssuA"
                     /locus_tag="OO349_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HCR5868943.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            1056..>1766
                     /locus_tag="OO349_004082"
     CDS             1056..>1766
                     /locus_tag="OO349_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCR5868944.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAY
                     GRKIRFGIRLHVIVRETND"
BASE COUNT          405 a          481 c          471 g          409 t
ORIGIN      
        1 tgcaaaccca tcttgatacc gcgctagaaa ctttctggca ggcattgcac cgccgcgatg
       61 ttcaggttcc tgaccttctg tctctgcgag gtaatgccca tgcgtaacat cattaaactg
      121 gcgctggcgg gattgcttag cgtctctact tttgcggttg ctgcagaatc ctcgcctgaa
      181 gcgttacgta taggctatca gaaaggcagt attggtatgg tgctggcaaa aagccaccag
      241 ttactggaaa aacgctatcc gcaatcaaaa atctcctggg tggagttccc cgcgggtccg
      301 caaatgttgg aagcgttaaa cgttggcagt attgatctcg gcagtaccgg ggatattccg
      361 ccaatctttg cccaggctgc cggggctgat ttggtatatg tgggcgtcga gccaccgaag
      421 cccaaagccg aagtgattct ggtggcagaa aacagcccga tcaaaaccgt agccgatctt
      481 aaaggtcaca aagttgcctt tcagaaaggt tccagttcac acaacctttt actgcgtgca
      541 ctgcgtcagg ccggacttaa attcaccgat atccagccca cttacctgac gcctgccgat
      601 gcccgcgccg cgttccagca aggtaacgtt gacgcctggg ctatctggga tccctactac
      661 tccgctgcgt tattacaggg cggcgtgcgg gtgctgaaag acggcaccga tctcaatcaa
      721 actggatcgt tttatctggc agctcgtccc tatgcagaaa aaaacggcgc ttttattcag
      781 ggcgtactgg caacctttag tgaggccgat gcgttaaccc gcagccagcg cgaacaaagc
      841 atcgctttac tggcaaaaac gatgggctta ccggcaccgg tgattgcctc ttacttagat
      901 catcgccctc ctaccaccat caaaccggtt aacgccgagg ttgccgcctt acagcagcaa
      961 acggcagatc tgttttatga aaaccgtctg gtgccgaaaa aagtcgatat tcgccagcgc
     1021 atctggcagc ccactcaact ggaaggaaaa caattatgag tctgaatatg ttctggtttt
     1081 taccgaccca cggtgacggg cactatctgg gaacggaaga aggttcacgc ccggttgatc
     1141 acggttatct gcaacaaatt gcgcaagcgg cggatcgtct gggctatacc ggtgtgctga
     1201 tcccaacggg gcgatcgtgt gaagatgcgt ggctggttgc agcgtcgatg atcccggtga
     1261 cgcagcggct gaagtttctt gtcgccctgc gtcccagcgt aacctcacct actgttgccg
     1321 cccgccaggc cgccacgctt gaccgtctct caaatggtcg tgcgttgttt aatctggtca
     1381 caggcagcga tccacaagag ctggcaggcg acggcgtgtt ccttgatcat agcgagcgct
     1441 acgaagcctc ggcggaattt acccaggtct ggcggcgctt attgctgggg gaaaccgtca
     1501 atttcaacgg taaacatatt catgtgcgcg gagcaaaact gctcttcccg ccgattcaac
     1561 agccttatcc tccgctttac tttggcggat cgtcagatgt cgcccaggag ctggcggcag
     1621 aacaagttga tctctacctc acctggggcg aaccgccgga acttgttaaa gagaaaatcg
     1681 aacaagtgcg ggcgaaagct gccgcgtatg ggcgcaaaat tcgtttcggt attcgtctgc
     1741 atgtgattgt tcgtgaaact aacgac
//
LOCUS       DAJTQY010000232         1717 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.238, whole genome shotgun sequence.
ACCESSION   DAJTQY010000232 DAJTQY010000000
VERSION     DAJTQY010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.238"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(110..577)
                     /locus_tag="OO349_004083"
     CDS             complement(110..577)
                     /locus_tag="OO349_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HCR5868945.1"
                     /translation="MYGTWSLSMAAIWLLRVDLLFVALFLIFISWGDILLRIISHRYL
                     LVLTVLIFLALILQHQKTNLVAAGSVLLVGFFLFSAGIIGGGDVKLLTVLSLAIDEHE
                     LANFLVAMTFCGALVVLAGLLFFRKSIRENGVPYAVPISLAFLLTYPVFPLFC"
     gene            complement(643..882)
                     /locus_tag="OO349_004084"
     CDS             complement(643..882)
                     /locus_tag="OO349_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HCR5868946.1"
                     /translation="MNTFITKYYGKTKQCFARFAKDERGVTAIEYALIGVAMATLLAF
                     IFGDQNSGFLGAIKDAFDAIAAAIQQVTISGTSNP"
     gene            complement(1017..1622)
                     /locus_tag="OO349_004085"
     CDS             complement(1017..1622)
                     /locus_tag="OO349_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR5868947.1"
                     /translation="MSDDRFYFSLGQGVKFYPASRRITTSTGNVINLSENGYRFLLLL
                     LQGESDKQHIINEVWREQKGSVSDSSYYGQIYMLRKALDVAGLSSSLIKTIPRKGVRY
                     LGKVEKHVCENERDSDEDSSSYTNDSEAREVNTDANIPENDNSQEFSTEELLPIKHTD
                     KNIEWYRTKQWSVFISVLTVLAVCWLTTLVFLVLLFLNQDK"
BASE COUNT          549 a          379 c          303 g          486 t
ORIGIN      
        1 gcttaggcct gtgtccatat tacgtgggta ggatcacaag acctactgct atcacaatta
       61 ctgagagaaa aaaaagcatc cgatgattca tttatgctgt cctaaaaaat taacaaaaca
      121 atgggaaaac aggatatgta agcagaaaag ccaatgaaat tggtacagca taaggcacac
      181 cgttttcacg aattgatttt cgaaaaaaaa gaagacctgc cagaacaacc agagcgccgc
      241 aaaaggtcat tgcgacgaga aaattagcca gttcatgttc atcaatagct aacgaaagaa
      301 cagttaacag cttgacatca ccgccgccaa ttatccctgc ggaaaaaaga aagaagccca
      361 cgagcaaaac gctcccggct gcaaccagat tggttttttg atgctgtaaa atgagtgcca
      421 aaaaaataag cacggttaac accagtaaat aacgatggct aataattctg agtaatatat
      481 ccccccatga aataaaaata agaaagagcg caacaaataa aagatcgact cttagtaacc
      541 atattgctgc cattgaaaga ctccatgtac catacaaggt tgctgccaga catattcctt
      601 tacgtctgac agcctctaga gagttttttt attttttata agttacggat tactcgttcc
      661 ggaaatcgtt acctgttgaa ttgctgcagc gatagcgtcg aatgcatctt taattgcgcc
      721 caggaagcct gagttctgat cgccaaagat gaaagcaagc agagttgcca tagcaacacc
      781 aatcaatgca tattcgattg cagtaacacc acgttcatcc ttcgcgaaac gcgcaaagca
      841 ctgtttagtt ttaccatagt acttagtaat aaaagtattc atgttttcct cgtatattat
      901 tatttaaatt caaaccacca gcttcatctg cgtgtcaccg gtatcccata ttttttggat
      961 gtggctataa ttatccattc ccgctattta gtcttcaaaa aaaattacat ttaatatcat
     1021 ttatcttgat taagaaatag taagactaaa aaaacaagcg tcgtcagcca gcacactgct
     1081 aacacggtaa gaacgctaat aaaaacactc cactgcttcg ttctatacca ttcaatattt
     1141 ttgtcagtat gttttattgg gagcagctct tcagtagaga attcctggct gttatcattc
     1201 tcaggaatgt ttgcatcagt gtttacttcc ctggcctcgc tatcgttggt atagctacta
     1261 ctgtcttcat cactgtcacg ctcattctca cagacatgtt tctcgacctt tcccagatat
     1321 ctgacgcctt ttcgcggaat ggttttaatt agagaactcg acaaacctgc gacgtctaat
     1381 gcctttctaa gcatgtaaat ctgcccataa taactgctgt cgcttaccga acctttctgc
     1441 tcccgccaaa cctcattgat aatatgttgc ttatcggatt ctccctgtag taaaaggagt
     1501 aaaaatcgat agccgttttc agacagattg attacattgc ctgtactggt ggtgatacgt
     1561 cgagacgctg ggtaaaactt aactccctgc cctaaagaaa aataaaaacg gtcatcgctc
     1621 ataagtaccg acccctgttt tcttcttcac atgactactc attacaactt tgtatgaagt
     1681 aagatgcaaa atcatattgt ttacattaag ttaagca
//
LOCUS       DAJTQY010000233         1638 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.240, whole genome shotgun sequence.
ACCESSION   DAJTQY010000233 DAJTQY010000000
VERSION     DAJTQY010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.240"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            <1..285
                     /locus_tag="OO349_004086"
     CDS             <1..285
                     /locus_tag="OO349_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR5868948.1"
                     /translation="SQSVTKMITCGENGHPVIVDRPSPTPEQLALINDEKKSALIAEA
                     TNVIAPLQDAVDLGMATDDETKLLLAWKKYRVLLMRVNVVKPEWPMHPNK"
     gene            complement(576..1577)
                     /locus_tag="OO349_004087"
     CDS             complement(576..1577)
                     /locus_tag="OO349_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCR5868949.1"
                     /translation="MHKSNCFDTARLVAAMMVLISHHYALSGQLEPYIFGFESAGGIA
                     VIIFFSISGYLISKSAIRSDSFIDFMAKRARRIFPALVPCSVLTYFLFGWMLNNFSTE
                     YFSHDIIRKTISSILMSQAPDADITSHLIHPGINGSLWTLPLEFMCYIITGVAVALLK
                     SGKTFIVILLIFVSLSLVESVSENKDVIFSIPVWLYPLRGMAFFFGATMAMYEKSWNV
                     SNVKISVISLLAMYAYASYGKGIDYTMACYILVSFSTIAICTSFGDPLVKGRFDYSYG
                     VYIYAFPVQQVIINTLHIGFYPSMLLSAIVVLFLAHLSWNLIEKRFLTRSSPKLSLN"
BASE COUNT          549 a          324 c          319 g          446 t
ORIGIN      
        1 tcacagagtg taacaaaaat gattacctgt ggtgagaacg gtcaccctgt cattgttgac
       61 cgtccttctc caacaccaga acaattagcc ttaataaatg atgaaaagaa atctgcactg
      121 atagcagagg caacgaatgt aatagcgccg cttcaggatg cggttgattt aggtatggca
      181 acagatgatg aaacgaaact gttactggca tggaaaaaat atcgcgtact gcttatgcgt
      241 gttaatgtag taaaacccga gtggcctatg catccaaata aatagacttg ttaatctagg
      301 gtaattgttg gaataatacg catttttcaa caggtccaga taacctggat ataatgctca
      361 cttctacaat ggcctcaact tattttattt aattgatatt acggaagtta actcatacat
      421 aaacaatatg actcagtact gtactaaata gctgcgtgga aaagaactcc accttcgctc
      481 gtccttgcag gacgaggcat cagaacggtg tccactgcca ggcagtgtta tatctggctt
      541 tgatttattc aggggctcga tggccccttt attattcaat tcagggataa ttttggcgag
      601 cttcgtgtaa ggaatctttt ttcaattaga ttccaagaaa gatgagccaa gaaaagaacg
      661 acaatggcac ttaacaacat cgaagggtaa aagccaatat gcaatgtatt tataataacc
      721 tgctgaacgg ggaatgcata aatgtataca ccataagagt aatcaaacct acccttaacc
      781 agtggatctc caaaagatgt gcatatagct attgttgaga aagaaaccag aatatagcaa
      841 gccattgtat agtcaatgcc tttgccatag ctcgcgtatg cgtacatagc aagcaaagaa
      901 atcacggaaa ttttcacatt acttacattc caagatttct catacatcgc catagtggca
      961 ccaaaaaaga aagccatccc ccgcaaagga tacagccata ctggaatgga gaatattaca
     1021 tccttgttct cagatacact ttcaactaat gaaagtgaaa caaaaatcaa tagtataact
     1081 atgaacgttt ttccgctctt tagtagagca acagccactc cagttattat gtaacacata
     1141 aactccagtg gtagtgtcca caagctgccg ttaataccgg gatgaattaa atgtgatgta
     1201 atgtcggcat ctggagcctg actcattaaa atagagctta tggtcttccg gataatgtca
     1261 tgactaaaat attctgtact gaagttgtta agcatccacc caaataaaaa atacgtcagt
     1321 acagagcaag ggacaagagc agggaatatt cttcttgctc ttttagccat gaaatcaata
     1381 aaggaatcac ttctgattgc tgattttgaa atcaggtatc ctgatatgga aaagaatatg
     1441 ataacagcaa tgccgccagc actttcaaag ccgaatatat acggctcgag ttgtccagag
     1501 agcgcgtagt ggtgactgat tagaaccatc atagctgcca caagccttgc cgtatcaaag
     1561 cagttgctct tatgcattaa aataaaatcc ctaagttttt gtgaggaaaa tatcagatct
     1621 ttatgtcata gtccattt
//
LOCUS       DAJTQY010000234         1601 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.241, whole genome shotgun sequence.
ACCESSION   DAJTQY010000234 DAJTQY010000000
VERSION     DAJTQY010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.241"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(334..480)
                     /locus_tag="OO349_004088"
                     /pseudo
     CDS             complement(334..480)
                     /locus_tag="OO349_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            534..716
                     /locus_tag="OO349_004089"
     CDS             534..716
                     /locus_tag="OO349_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein"
                     /protein_id="HCR5868950.1"
                     /translation="MNHPVIGVVTKADLASMEQISLVKSWLREAGAHNVLVTSAVNNN
                     GVTELFALLHTEEGCC"
     gene            complement(817..1146)
                     /locus_tag="OO349_004090"
     CDS             complement(817..1146)
                     /locus_tag="OO349_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HCR5868951.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(1318..>1601)
                     /locus_tag="OO349_004091"
     CDS             complement(1318..>1601)
                     /locus_tag="OO349_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5868952.1"
                     /translation="FVLLNAQKLCDTQHMMQQILLSLVHALYEGNPQPVFANTEKLND
                     AVEELRQLLDNHHDLKVVETPIYGYVWLNMETAHQLELLSNLICRALRK"
BASE COUNT          436 a          368 c          375 g          422 t
ORIGIN      
        1 tagcaatgtc tgtcgcacaa caaccacaat gtcacaaata aaaaatgaga ataaccagtt
       61 ccctgaaggc gacgtagccc ggtacaagag taacccacgt tagcgcttgg cgacggcgga
      121 acaaagctga ttcgcgacgt aagaaagtgg caggctcata tgacggacct ctgtaatgcg
      181 gtatccaacc cgcgaatatc agcatgattc accgtcggat ttactgctac gtcgccctca
      241 gggaattaaa cctaaaaaga aaatcgtcaa aattagagat tccggtttga cacggaaagt
      301 catatcagcg tcaacggagc accgtattga cgcttattta ttggtgagaa ctacgttcca
      361 tggcaggagt tcgtcaacac ggttggaggg ccattcaggc agtatgctca gaatatggcg
      421 cagatacgct tccggatcga taccgttcag acggcaggtg cctgcttttc cggcaacaag
      481 caggaatctc agcgctgaag caggaattag aggtaaaaac gccctaccgg gccatgaatc
      541 acccggtcat tggagtagta accaaagcag atttagccag catggaacaa atatctttgg
      601 tgaaaagttg gttacgggaa gctggagcgc acaacgtgtt agtaaccagt gcagttaata
      661 acaatggagt tacagaactt tttgccttgc tgcatacaga agaaggctgt tgttaatttc
      721 gattggttct cagatgagaa tgatgacggt gatacacaga taagaatcaa tgagcaatga
      781 gggttgccgg gcaaccctca ttgaataaaa cgggaattac ttcgcttcgc cgtttttcat
      841 ttcgcccata gctttcagct ttttggagat atcgcggcgt tctttggaga gctcggcatt
      901 tttgatgatg taatcgtcaa cgcgatcttc atagtcacct ttcatgctgg cgatgatgcc
      961 ctggattgct tcaacgctca tccctggctt gatgtaatcg ctcaggttgt ccagcaacag
     1021 gacgcgcttc tggttgtcac ggatcttttt ctcaacgtcc tgaatttcac gttgcagttt
     1081 gttcttacga cggaacagac gaacaaattc cagtacgtcc tggaatgaag gcttggtagt
     1141 ttccattttt atacccctga taatgtgaga gtcggattcg tttaagcaac cgctattcgt
     1201 tagggccaac attactcata gctgccgcag atgacaatgc ttttatccct tttcactatc
     1261 atacccttta tccttgctga atcgaagcag cagcaagatg attctgaagt tcagaaatta
     1321 tttgcgcaag gcccggcaaa tcagattcga tagcaactca agctgatgtg ccgtttccat
     1381 gttcagccac acataaccat agattggtgt ttccacgact ttcaggtcat ggtggttatc
     1441 gagcaactga cgcagctctt ccacagcatc gttcaatttt tccgtattgg caaaaaccgg
     1501 ctgcggatta ccttcgtaca gcgcatgaac aaggctcagc agtatttgct gcatcatgtg
     1561 ctgggtatca caaagttttt gcgcgttcaa taacacgaaa t
//
LOCUS       DAJTQY010000235         1600 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.242, whole genome shotgun sequence.
ACCESSION   DAJTQY010000235 DAJTQY010000000
VERSION     DAJTQY010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1600
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.242"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            452..1300
                     /locus_tag="OO349_004092"
     CDS             452..1300
                     /locus_tag="OO349_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCR5868953.1"
                     /translation="MLNVAIENQNGWNYSAPAPHKTGAGIATPTMTTAHNRAQAVFLC
                     VKHSHIQIMVGRAGQPQGWPVSVVTGCSNPVRLTTHEIATSGGESFKLTIEAAIMATI
                     LTLSHPDATIENGRAVTTSVAVAEFFRKMHKNVIQKIETLECSPEFNRLNFKPVTYTD
                     AKGEKRPMYQITKNGFVFLVMGFTGKKAAAFKEAYIAEFDRMEAELRQNNTPPADKMI
                     PGDGRTLVVHFDKFGNVEFTETVPDGALVCTLETFRFYLEKQGWTLVNRGAIKNMTVE
                     QLLSLK"
     gene            1317..1598
                     /locus_tag="OO349_004093"
     CDS             1317..1598
                     /locus_tag="OO349_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5868954.1"
                     /translation="MNPMLDYCFTSAPQQTITHTGIKNLLWIIKKNLQQKGDPDLIPC
                     AYGLCDRQVSPDKAYTAELIYMHGTMMRKKKRKYCSEICAEKDQMAHEL"
BASE COUNT          444 a          370 c          381 g          405 t
ORIGIN      
        1 cattatatag ttctactctg gcgagatacg ttggcatatt catttcctta ctggttgtgt
       61 gagaacttca gtaaagatac caccaaagcc cggaagtggt gaaataaaac cgggcacaac
      121 acgaaggcgc atttccggta tccataaaga gtcggtcttg tctgtgaaat ttaaatggtg
      181 ggagtgcgct tccggttgta aataacgaca ttgctgtgtg aagtaccagt tggcggcatc
      241 ggtttaattg ctggctgatg tccgcccttt ttaaagtgaa ttttgtgatg cggtgaatgc
      301 ggctatgcgc acgcggcaca gttaaaagtc atgttagtcc ttattggttt gggtgggaaa
      361 gccaactgta attgttaact ggttaacgtc acctggaggc accaggcacc gcatcaacaa
      421 agttcacttc ggtgatgaaa ggtaagagaa aatgttgaat gtagctattg aaaaccagaa
      481 cgggtggaat tatagtgcac ctgcacctca taaaacgggt gccgggattg ctaccccgac
      541 gatgactact gcgcataacc gcgctcaggc ggttttttta tgcgtcaagc acagccacat
      601 tcagattatg gtggggcgtg cagggcagcc gcaaggctgg ccggtttcgg tagtcaccgg
      661 ttgtagcaac cctgtgcgtc tcaccaccca tgagattgct acctctggtg gtgagtcatt
      721 taaactgact atcgaggctg ccatcatggc tactatcctt accctttctc accctgacgc
      781 aaccatcgaa aatggtcgcg ctgtcactac gtctgttgcg gttgcggagt tcttccggaa
      841 aatgcacaaa aacgttattc agaagatcga aactctcgaa tgctcgccgg agtttaaccg
      901 gcttaatttt aagcccgtca cctataccga cgccaaaggc gaaaaacgcc caatgtacca
      961 aatcaccaaa aacggcttcg ttttcctggt gatgggcttc actggcaaaa aagccgccgc
     1021 attcaaggaa gcctacattg ctgagttcga ccgcatggag gccgaactgc gccagaataa
     1081 caccccacct gctgacaaga tgattccggg tgatggtcgc actctggttg ttcacttcga
     1141 caaattcggc aatgtcgaat tcaccgaaac cgttcctgat ggcgcactgg tctgtaccct
     1201 ggagactttc cggttttatc tggaaaaaca gggatggact cttgttaacc ggggcgcaat
     1261 taaaaatatg actgtggaac aattactttc tttaaaataa aaatggagct acggatatga
     1321 accctatgct tgattattgt tttacctctg cacctcagca gaccataaca cacaccggaa
     1381 tcaaaaattt gttatggata atcaaaaaaa acctccagca gaaaggtgat cccgatttaa
     1441 ttccctgtgc ttatggcctg tgtgacagac aagtaagccc tgataaggct tacactgctg
     1501 aacttattta catgcacgga accatgatga ggaaaaagaa acgcaaatat tgtagtgaaa
     1561 tctgcgccga aaaagaccag atggcacacg aactttaatt
//
LOCUS       DAJTQY010000236         1570 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.243, whole genome shotgun sequence.
ACCESSION   DAJTQY010000236 DAJTQY010000000
VERSION     DAJTQY010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.243"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            <1..189
                     /locus_tag="OO349_004094"
     CDS             <1..189
                     /locus_tag="OO349_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5868955.1"
                     /translation="MLFGASLIMVVGCSSKENALCHPQPKPPAPPAWAMMPPSNSLQL
                     LDETFSVSGTESSATRQH"
     gene            complement(322..834)
                     /locus_tag="OO349_004095"
     CDS             complement(322..834)
                     /locus_tag="OO349_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HCR5868956.1"
                     /translation="MMEWINALMNYFVLHPYRLWGLLFIIAFTKSTLLISAILPPASI
                     MMATVITVSKTTLSPWEAGITVMSGAWCGTIVNYHLGIIIGHIPQLACIISSRSNTIE
                     RVRLQLQNNSVSILFTSRFIAVLRYIAPLVAGMLQLHPVKVYTVSLISAASWSALYVG
                     SFSFVLPFFS"
     gene            1029..>1570
                     /locus_tag="OO349_004096"
     CDS             1029..>1570
                     /locus_tag="OO349_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5868957.1"
                     /translation="MAKPDWEAIESAYRAGVLSLRDIGEKYGVTEGAIRKRAKKLDWA
                     RSGGTQVCKNGTQKRKVRTSRKPAITGLTQKSTQLKTESTPDTKPIRGMRTDPPTNPF
                     QPGNQQALKHGGYARRLLLKDEVIEDAKALTLEDELFRLRANNLVAAENIGRWLVSLE
                     DANGDQERKMLIENISAAEKA"
BASE COUNT          468 a          352 c          394 g          356 t
ORIGIN      
        1 atgctcttcg gcgcaagctt gataatggtg gtcgggtgct cgtcaaagga aaatgccctg
       61 tgccatcctc agccgaaacc tccagcgcct ccggcatggg caatgatgcc accgtcgaac
      121 tctctccagt tgctggacga aacgttctcg gtatccggga cggaatcatc agcgaccaga
      181 cagcattgag aatgcttcag gagtacatca ggatccaatg ccttgggggg tagcggtaat
      241 tttactcatt atccttcaaa tcagattctg ttgtcagagg aatgggggaa gctggattcg
      301 caagagcttt ttatgggcag tttagctaaa aaaaggcaac acgaagctaa aactaccaac
      361 ataaagtgct gaccagctag cagctgaaat cagactcact gtatacactt tcactggatg
      421 aagttgcaac attcctgcca ccaaaggagc aatgtaacgc agcacggcga taaagcgcga
      481 agtgaaaagt attgacacag agttattttg caactgaagg cgaacccgtt ctattgtatt
      541 ggaacggctc gatattatac atgcaagttg agggatatgc ccaattatta taccaagatg
      601 gtaattaacg atagtaccgc accatgcgcc actcatgacc gtgattccag cttcccatgg
      661 tgaaagagtc gtcttgctga ctgtaattac agtggccatc ataattgatg ctggtggcaa
      721 tatcgccgaa atgagtaaag tggattttgt gaacgctatt ataaaaagaa gcccccatag
      781 gcgatatgga tgaagtacaa aatagttcat caaagcattt atccactcca tcaatgtctt
      841 tccttctgtg aatttttctt tgctatttaa ccgtgatacg acttaattta ggcttaaaca
      901 ccttacattt acaaggtact cctgtggggg gccctgccac ggagcgtcgc aggagtgagt
      961 gatgaggaag aattaaagca acctattgtg acaggcagcg ataacagtaa atcacaggaa
     1021 gcataatcat ggcaaaaccg gactgggagg ccatcgaatc ggcataccgg gccggagtcc
     1081 ttagcctccg tgatataggc gagaaatacg gcgttacaga aggggctatc aggaagaggg
     1141 ccaaaaagct tgattgggca cgcagtggcg gtacgcaggt ttgcaaaaat ggtacgcaaa
     1201 aaaggaaagt gcgtaccagc agaaagcctg ccattactgg ccttacacaa aaaagtacgc
     1261 aactaaaaac agaatctaca ccggatacga aaccgatacg cggaatgcgt accgatcccc
     1321 cgactaaccc attccaaccc ggtaaccagc aggcattaaa acacggtggt tatgcccgtc
     1381 gccttctgct caaagatgag gtgatagagg acgctaaagc gttgacgctc gaggacgaat
     1441 tatttcgcct tcgtgctaac aaccttgtcg ctgcagagaa tattggtcgg tggttggtgt
     1501 cgctggaaga tgctaatggg gaccaggaaa ggaagatgct gattgaaaat atcagcgccg
     1561 ccgagaaagc
//
LOCUS       DAJTQY010000237         1554 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.244, whole genome shotgun sequence.
ACCESSION   DAJTQY010000237 DAJTQY010000000
VERSION     DAJTQY010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.244"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(<1..545)
                     /locus_tag="OO349_004097"
     CDS             complement(<1..545)
                     /locus_tag="OO349_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001338841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5868958.1"
                     /translation="MDYFMKGKSALTLLLAGIFSCGTCQATEAEVTSESVFNILNSRG
                     AATDKSYLSLNPDKYPNYRLLIHSAKLKNEIKSHYTKDETQGLLTLTENTRKLTLTEK
                     PWGTFILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWGYM
                     STPDQIKRMQDIISNPKRLDG"
     gene            complement(1200..>1554)
                     /locus_tag="OO349_004098"
     CDS             complement(1200..>1554)
                     /locus_tag="OO349_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5868959.1"
                     /translation="LVPEKLEAILDRAAQRAGHEGISELLGCTVCGYAGDDTGVYLLP
                     KRFASISFRSTKDAKTVDVKVTRNSNTAGYDLELISVVDVLATGSVKVKAGEFDVEED
                     ASFPLVHVIRFTAAE"
BASE COUNT          448 a          342 c          325 g          439 t
ORIGIN      
        1 attccatcca gtcgttttgg gttgctaatg atgtcctgca tccgcttaat ctggtcgggc
       61 gtagacatat aaccccacag ggtgagcaga accttttttg cggctacact atttccttgg
      121 tctgatacta atgccatata gccccataaa ctatccctca gccagacagc atcatagtgg
      181 gtttctgccg ctgttttgtc atcttcgaaa gtagaagcaa gaataaacgt tccccagggt
      241 ttctccgtca acgttaactt tcgtgtgttt tctgttaatg ttagcaatcc ttgagtttcg
      301 tctttggtat agtgactctt tatttcattt tttaatttgg ctgagtgaat cagaagacga
      361 taattaggat acttatctgg atttaacgat aaataacttt tatctgttgc tgcccccctg
      421 gaattaagaa tgttaaatac agattcgctt gtcacttctg cctctgtcgc ctggcatgtg
      481 ccgcacgaaa atataccagc gagcagaaga gttaatgcag atttcccttt cataaaataa
      541 tccatcaatc gtttgttaaa agcgtagata accacttatt caacaacttg aaaataaggt
      601 attttacctg ggttgttaca aaaggattgc attgcgtaaa cgctttttat ttacaaaaaa
      661 atggggaagt gttacagcga ttgcgaaaag cctgaagtga ttgaattaag ataatataga
      721 aaatcagata cagcaaaaat ggcagattga tcaggtgaag ataaattgga gcgggcgaag
      781 ggaacctagg tatcgagggt atcgaagata atcagccgct aaccatttgt ttccttaaag
      841 gatgatgaat ccttgtggtt acagttatgg ttacacatta atttatctct ggtcaagtcc
      901 cgaagtccta actttaagac agtggcccca atcgtccaga cttgaaaggg gcctatgggg
      961 gaagtcgctg acctttatct attaaatacc tacagagatt tttgtgagat tttttaatcc
     1021 aaggtcataa cctactgccc ccacaggccg caaagtgaag acctgtaagc accagagcaa
     1081 tagcgagtat ctatcactcc ctgtagagta tctggtagac caagaggaca cagaggacga
     1141 ccatatacaa ctcctgtaag ctcatggatg tactccttat gttcactcag ggtttatagt
     1201 tactctgcgg cagtaaaacg aataacatgg accaacggga aagaggcgtc ttcctcaacg
     1261 tcaaactcac ctgctttcac cttaacggaa ccagtagcca acacatcgac cacactgatt
     1321 aactcaaggt catacccagc agtgttgcta tttcgggtca ctttgacatc taccgtctta
     1381 gcgtctttag ttgacctgaa agagatagaa gcgaaccgct taggcaggag atagacacct
     1441 gtatcatcgc ccgcatagcc gcatacagtg cagcccaata gctcactaat accttcatga
     1501 cctgcgcgtt gagcggctcg gtccaagatt gcctcaagtt tctcagggac caat
//
LOCUS       DAJTQY010000238         1537 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.245, whole genome shotgun sequence.
ACCESSION   DAJTQY010000238 DAJTQY010000000
VERSION     DAJTQY010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.245"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(909..1385)
                     /locus_tag="OO349_004099"
     CDS             complement(909..1385)
                     /locus_tag="OO349_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5868960.1"
                     /translation="MFIDSEKRLKQLSDEAKKNTEDLEEAKKNSRFTQVSPKGWERVR
                     ELLKDSQGISALKLYSFLAEHIDPTCGAVVADQQFLAEKLGVSRSTIIRWLNYLESKN
                     ALVRIPVAGKVCAYALDPHEVWKGYNTTKNHAAFVTKTLVNKDGDIQRRIMAMFSN"
BASE COUNT          348 a          410 c          385 g          394 t
ORIGIN      
        1 aaaaaaacaa caacttacaa atcggcgtaa aagatctatt ctaggcaagg gcgttgcgcg
       61 caacgcaacg ccctttgggg tgtcggggcg aagccctgac ccagtcacgt agcgatagcg
      121 gagtgtatac tggcttactc ctctgcaagc ctcgtcagcc gtgattttgc ccgatttcgc
      181 gctctccacg ctcgatcatg tttttgccgt ccgttcaccg ttaaaacgcc tcagaaatcg
      241 ggacaggatg tgggaatgtc ggaccgcgca cgcgcactca gacattcccc acttggtcag
      301 ggcttcaccc cgacaccccg catccgtgcg taagtgctca atgtaacttc cggttctctt
      361 ccgcttcctc gtcgattcgc tcgctacgct cggtcatcga cttgcggcga gcgggatcac
      421 ctctcgcatg ttgtcggtga tcccgttgtt gttctttcgg ggatgagtcg tcagatagcc
      481 cttcaaacac gctcagaagc tctcagacgc acgaaacggg tttaaatgcc cgtcagggca
      541 tggaaggcgc gaaaaacgct tacagaggct ctcaggaggt caggcgcgcc gataccccta
      601 accaggggct ttcaggtcgc caggaaccgc gtcagctccc tgcaaagcct atttgcacgg
      661 cacaggcagc tattcggctg gttggtggtc gtagaccgtc ggtgttacag cgatgggggt
      721 ctggggttct ccccagacgg tttccggttg cgctcaaggc gcagccggaa ccgtggcggc
      781 gcggtcgttc tggggatcgg ggagcaaaaa cacgcaaggg attttgaggc ggcaaaagag
      841 caatgcagga tagacaatca tggtcaaaac gttaacacgt agcccctgat tgtccgcctg
      901 ccgctggctc aatttgaaaa catggccatg attcggcgct gaatatcacc gtctttgttg
      961 accagtgttt tagtgacaaa cgctgcatgg tttttcgtag tgttgtatcc cttccagact
     1021 tcatgtggat cgagggcata cgcacaaacc ttaccagcaa cggggattct aactaatgca
     1081 ttttttgatt ctaagtaatt gagccaccga ataattgtgc ttctgctaac tccaagtttt
     1141 tcagctagga attgctgatc cgcaacgaca gcgccacacg taggatcgat atgctctgct
     1201 aaaaatgagt acagcttcag tgctgatatg ccttggctat ccttcagcag ctctcgaacg
     1261 cgttcccaac cttttgggga tacctgtgta aaccttgaat tcttctttgc ttcttcgaga
     1321 tcttcggtgt ttttctttgc ctcatctgaa agttgtttca gtcgtttttc tgaatcaata
     1381 aacatgattt tttccctctg aaaatcccat gcactaatgt tggttcaaat ctaagccatc
     1441 atgacacatc tgtgcaacac cttgttgcgt gttgtcgcat ccaggatgcg acaacgttgt
     1501 gtcaccctgg atgcgacagg taaacaaaaa acacact
//
LOCUS       DAJTQY010000239         1523 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.246, whole genome shotgun sequence.
ACCESSION   DAJTQY010000239 DAJTQY010000000
VERSION     DAJTQY010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1523
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.246"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(1..836)
                     /locus_tag="OO349_004100"
                     /pseudo
     CDS             complement(1..836)
                     /locus_tag="OO349_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935599.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
BASE COUNT          398 a          363 c          367 g          395 t
ORIGIN      
        1 gctgctctac ggtcatattt ttaattgcgc tccggtttac aagagcccac ccctgttttt
       61 ccagataaaa ctggaaagta tccagggtac agaccagtgc accgtcagga acagtttcgg
      121 tgaatctgat attgccgtgt tcgtcgaagt gcacaaccag agtgcgaccg ttacccggaa
      181 tcatcttgtc agcggacggg gtattattct ggtgcagttc tgcctccatg cggtcgaaca
      241 tcagcgatgt aggcctcttt gaatgcagcg gcttttttcc cagtgaagcc catcaccagg
      301 aaaacgaagc cgtttttggt gatttggtac attggacgtt tttcgccttt ggtgtcggtg
      361 taggtgacgt cctcaaaatt gagggcgtta aattcaggtg agcattcagt atttgcgatg
      421 gcacgtaaca cattgtcgtg tcgtttgtgg aagaactctg caaccgcaac agacgtagtg
      481 acagcgcgac cgttttcgat ggttacgtca gggtgagaaa gggcagggat agtagccatg
      541 atggcatcct cgagtgataa gttaattaac tcaccaccga ggttttccac gaccataagg
      601 gtggtgagac gtacaggggt ggaaataccg gtcactcgag aacccggcca gtcttgcgac
      661 tgccctgcac atcccaccat aatttgaatg gagctgtgca ttacgcataa aaaaaccgct
      721 tcagcgcggc tatgcgctcg agtaacgttc gggtttccac gcccggcccc cgttttatga
      781 ggtgcagatg cactataatt ccacccgttc tggttttcaa tagctacatt caacattttc
      841 tcttaccttt catcaccgaa gtgaactttg ttgatgcggt gcctggtgcc tccaggtggc
      901 gttaaccagt taacaattaa cgccggatac agggaaaccc ataaccaccc gatacgtggg
      961 aactaatcgc tttttaactg ttccgcgtgc gctcagccgc attcaccgca tcacaaaatt
     1021 cactttaaaa agggcggaca tcagccagca atgaaactga tgccgccaaa ggtaccaatc
     1081 aacatggagt gttgtggcgt ggttgtcact taagcgtatg gtcaacctga caacccggtg
     1141 tcctcaacgg ggaaggaata actccgccat acttaccgcc gcgtcatttc gcgttgtgtg
     1201 cctgctttta actacgtcag gcgaggtggt tcctgttatt ccccaacaac aagaaatttg
     1261 tataatctga ataccccaac aataagaaga gtgagcaagt gatgtattag cgatgtcagc
     1321 tctcaaagag actgcatcaa tgttaaaatt attcaatgaa gcaaaaactc aggctgaggt
     1381 tgatcgcgct gtgtctgaaa taaactctaa gttgtcaagc attcagcttg aatatttgtc
     1441 tctcattgat gagctgacaa tctctaaaag caatgaggct gcgctcaaag caaaactcac
     1501 ggagatagaa aagtttaagt tcc
//
LOCUS       DAJTQY010000240         1438 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.247, whole genome shotgun sequence.
ACCESSION   DAJTQY010000240 DAJTQY010000000
VERSION     DAJTQY010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1438
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.247"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(1..543)
                     /locus_tag="OO349_004101"
                     /pseudo
     CDS             complement(1..543)
                     /locus_tag="OO349_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001572122.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
     gene            complement(591..1163)
                     /locus_tag="OO349_004102"
     CDS             complement(591..1163)
                     /locus_tag="OO349_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5868961.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVNFVGKIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
BASE COUNT          428 a          303 c          277 g          430 t
ORIGIN      
        1 ctctgctatc agatttcgcc cttcccggcg ccatttcact ttcggcgcag catcttcggg
       61 aataaggcgg atatttgctg gtcgccagaa cagcttgata cgcgttttta ctgctatttc
      121 cagcgagttc gtcgctttgt tgctcgctga tggctgaata tttttaatat tcagccagaa
      181 gactgactcg cgatcatcag gcaatgacac acctttggtg tgaataatgc gtaatatttt
      241 ttcttgcgaa ggttctaatc gtgatactgg cggaataacg gtaaacggcg ctttgctttt
      301 atcctcttta ttaaatggat ctacccaagc ctggatgaga taaggtattt tttcttcttt
      361 gctaaataca gaaatagatt tatcagcatt attacttaag taaaccaccc gcgtactacc
      421 aatgacaacg cctgcataag ttaatgctgg ttggcctaaa aaaacgctga gtataatcgc
      481 ggtaaataac ttacattaaa ctgttgtttt aaagagcctg attaaacaac tttttctgga
      541 cataacgact ccctggccaa agccaactga ataaaaaggc ccttgataaa ttatttctta
      601 tattcgacag taaattgtga atcagcgttc gccgtaccag ccgttacggt cgcattggtt
      661 gccacatagc gtgcaataaa cttcatttca gccttgcctt cagcatcgat agcggtataa
      721 gcagagtcgt tataaagttt aacctgcgaa ttatcggact ggttaaaaat acgaatacca
      781 acgccggttg ccgcaatcgc ttgtcctgta ccggtaaaat caccagggtt gcctgctgta
      841 agcggcgtac ccactttcag gtcgccgtcg ccgccaggcg cttctgtacc atcaaaacga
      901 acggctgctt gagtgtacgt tgccgggcaa ttttcaagtt taatactgaa cttctgtggc
      961 gacgccagac tacctacacc tgaaaatgca tttttactga ctttacccag tgggacaatt
     1021 tgatcggctg tatcggaggt aacttcacaa gtagtgtcga caattttacc gacgaagtta
     1081 acaacaccat cttctgccag tgcatgcccg gccagcatta gagatgccag agtgaatgca
     1141 ccgcctataa tattacgctt cataaaaact ccattgatat aatatatcct ttaccatgaa
     1201 tcatcactta tattaataag tgtatttttg attcaaagga atagtacacc aaagagacgg
     1261 ctttatctaa aaataatatt aattacgcaa aaataaaata tagatttatt gtttaaatat
     1321 tatttaaaca gtaaaaaatc aagtttgtta tcagacatta atgataattc agaatatatt
     1381 tatatagaaa gatagatgaa tttattgatg tcagttataa aaaagggcca ccggtcgg
//
LOCUS       DAJTQY010000241         1392 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.248, whole genome shotgun sequence.
ACCESSION   DAJTQY010000241 DAJTQY010000000
VERSION     DAJTQY010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1392
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.248"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            63..251
                     /locus_tag="OO349_004103"
     CDS             63..251
                     /locus_tag="OO349_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5868962.1"
                     /translation="MLTRKSIDTVLLSVGAEKLSQREWDWMKMLKPMDPPPAMVAASI
                     LERRGDTAALTRLQDTGG"
     gene            261..>1392
                     /locus_tag="OO349_004104"
     CDS             join(261..1077,1077..>1392)
                     /locus_tag="OO349_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858395.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HCR5868963.1"
                     /translation="MLSAFTPRPLKRLFTANQCWTSFLDAGGLRDIEVEAVTKMLACG
                     TRILGVKEYICDKPECPHVRYVTNSCGSRACPSCGKKATDLWIATQLNRLPDCDWVHL
                     VFTLPDTQWPVFESNRWLLNDVCRLAVENLLYAARKRGQEPGIFCAIHTYGRRLNWHP
                     HVHVSVTCGGLNKHGQWKKLSFLKDAMRSRWMWNMRQRLLKAWSEGLAMPESLSHITT
                     ESQRRSLVLKAGGKYWHVYMSKKTAGGRNTARYLGRYLKKPPIAASRLAHYNGGGSLS
                     FRYLDHKTGETATETLTQRELVARLKQHIPEKFFKMVRYFGFLANRVCGEKLPQVYRA
                     LGMDKPEPVAKVCYAQMVKQFLSRDPFECVLCGGRMVYRRAIAG"
BASE COUNT          310 a          368 c          430 g          284 t
ORIGIN      
        1 gccgcacgct ccgcagaata tgtatgctct gaggacctgt tttattaagg actcatcctc
       61 ttatgttgac ccgcaaaagt atagataccg ttctgctctc tgttggtgct gagaagctct
      121 cacaacgtga atgggactgg atgaagatgc tcaaacccat ggacccacca cctgcgatgg
      181 tcgcggcctc catcctggag cgccgtggcg acacagcagc ccttacccgg ctacaggata
      241 ccgggggtta atccgtcctc atgttgtccg cttttactcc ccgcccgctg aaacgtctgt
      301 tcacggccaa ccagtgctgg acatccttcc tggatgcggg cggtctgcgc gatatcgagg
      361 ttgaggctgt cacaaaaatg ctggcctgcg gcacgcggat actgggcgtg aaggaataca
      421 tctgcgataa acctgagtgc ccccacgtca gatacgtcac taactcatgc ggcagccgtg
      481 cctgcccgtc ctgcggaaaa aaggccacag acctgtggat agcgacacag ctgaatcgtc
      541 ttcctgactg cgactgggta cacctggtct tcaccctgcc ggacacgcag tggccggtgt
      601 tcgaaagcaa ccggtggctg ctgaatgacg tgtgccgtct ggcggtggag aatctgctgt
      661 atgccgcccg gaaacggggg caggaacccg gtatcttctg cgccatccac acgtatggcc
      721 gtcgtctcaa ctggcatccg catgtacatg tgtctgtaac ctgtggaggt ctgaataagc
      781 atggtcagtg gaaaaagctg agcttcctga aagacgcgat gcgttcgcgg tggatgtgga
      841 atatgcggca acggcttctg aaagcgtggt cagaggggct ggcgatgccg gagtcgttgt
      901 cacatatcac gacggaatca cagaggagaa gcctggtgct gaaagccgga ggaaaatact
      961 ggcatgtgta catgtcgaaa aaaacggccg gcgggaggaa tacggcgcgc tacctgggtc
     1021 gttatctgaa gaagccgccg atagcggcct cccggctggc acattacaac ggagggggag
     1081 cctgagcttc cgttacctgg accacaaaac gggagaaacg gcgacggaaa cgctgacaca
     1141 gcgtgagctg gtcgcgaggc tgaaacagca catcccggag aagtttttta agatggtgag
     1201 gtacttcggg ttccttgcca accgggtgtg tggagagaag ctgccgcagg tgtaccgtgc
     1261 tctggggatg gataaaccgg aaccagtggc gaaagtgtgc tatgcacaaa tggtgaaaca
     1321 gttcctgagt cgtgacccgt tcgaatgcgt gctgtgtggc ggccggatgg tataccgccg
     1381 ggccatcgcg gg
//
LOCUS       DAJTQY010000242         1317 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.250, whole genome shotgun sequence.
ACCESSION   DAJTQY010000242 DAJTQY010000000
VERSION     DAJTQY010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1317
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.250"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            119..916
                     /locus_tag="OO349_004105"
                     /pseudo
     CDS             119..916
                     /locus_tag="OO349_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130996.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
BASE COUNT          399 a          238 c          304 g          376 t
ORIGIN      
        1 taatttaagt tcagcagcag ttaaaacagc aggtgtactt atcagctttc aggctgtact
       61 atccgcttat cagcgagtaa tggaagttgg ccttcaacgt gcaagtgctg aacgttcaat
      121 tgatttcgta ttttctaaag atgcaaatca agtaaaagat ttcacaaagt cattagctca
      181 aacaacgggt ttagatatcg ctgaactgca atcacagttt gctggtttcg gtgcttcagc
      241 tagagagtct ctaggcattc aaggttctga agaacttttt agaaatatga ttggctattc
      301 tcgtctgatg gggcgaagtg aagaagagat taagcgagca ctgacagcat tatcacagat
      361 ggctggtaag ggtcagatta tggctgaaga gcttaagggg caactagctg aagctgttcc
      421 tggtatggtt caggtattcg ctaaagcaac cggaaagact gaacaggaat tatttgatgc
      481 tatgaagaag ggggctttaa aatctgccga tactcttcag aaggtcactc aggaactgaa
      541 taaacaaatc accgctaaag gtggctggaa agctatttca gaaagtacac aggcacaact
      601 agggaacttg aaaaactctt ggaataccac gcttgatagt atcttcagag gttctgaaaa
      661 tggtctgcaa gattttacaa gaagcttaac caatctttta aacgctcttg gtggaagtgg
      721 taagagtctt ggtgaatctc ttggtagtct catgacttct atgtcacatg gtgtcgattc
      781 attgaccgat attagttaca gggtaagggc gttctttgat gaggtgacat tagcttaccg
      841 tccactaaat gacactcaga ggaaaattgt tgatggttta tctggtgggt taattgatgg
      901 tctgaagatg cttgctgtgg ctgttactgc tcaaaaggct gcaaatgtag caggaggctt
      961 gttaaacctt gcaaacgctg ttacttcatt cggtgacgct gttaataaag ctaaagctgg
     1021 caaggctggt tcttcaggtg gtgcgctaaa taaagtcggt agagttgtcc tttatgctga
     1081 gttggtaaat tttgctcttg ataatttaaa ccctgaagca gctaagaatc cgaacttaca
     1141 tcaatcaggt attggcacag aaacaaactt caaccctgaa tacaaaggtg attcatctaa
     1201 gccaatagtt ctatcgctga ctggtttaat ggtgcaacta ataaaattgc taacttccaa
     1261 gaaccgatga ataacttcgt aaataacaca caatcaactc catcagctat cacaggt
//
LOCUS       DAJTQY010000243         1298 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.251, whole genome shotgun sequence.
ACCESSION   DAJTQY010000243 DAJTQY010000000
VERSION     DAJTQY010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1298
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.251"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            <1..698
                     /locus_tag="OO349_004106"
     CDS             <1..698
                     /locus_tag="OO349_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5868964.1"
                     /translation="RAGVPGSDEETRADFENRRRNSVARNARNILEAIRGEILSTVEN
                     VVDVYVTHNPKKTEQKAGVSQYPLTPGSLYVGVYGGSPADIAAAIWRKAPPGIDMNGN
                     TTFTVADKEYDPPYPEYVISWQTLKPVSLHVSVTLKKSDYLPSDITRQVQQSVLDAFN
                     GTDGGLRARVASVVSAGRYYAGIYKTDPEHIDILGLTVSRDGSSWTTAVTFGIDEIPV
                     LDVSDISVKLQEA"
     gene            709..>1298
                     /locus_tag="OO349_004107"
     CDS             709..>1298
                     /locus_tag="OO349_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
                     /protein_id="HCR5868965.1"
                     /translation="MAATVLAQYAASPRLNALINSFNAALSPDSFISDFYGLIWNIDT
                     AEKYGLDVWGKIVGVSCWLTVKDDFNYLGFSESRMDTPVMDDPCPFNQAPFYNGKSDT
                     RTVDLSDAVYRRLILMKAMSNITDCSVPDINLMLRFMFGKKRRAYVLNNGGLRMSYVF
                     ESALSLAELAIIQSSGALPSPPGVYVSVVLKESRNEG"
BASE COUNT          309 a          308 c          352 g          329 t
ORIGIN      
        1 cccgtgccgg tgtgccgggc agcgatgagg aaacccgtgc ggactttgaa aaccgccgac
       61 gtaattcagt tgcccgtaat gcccggaata ttctggaagc catccggggt gaaatactct
      121 ccacggtaga aaacgtggtg gatgtttacg tcacccataa tccgaaaaaa acagaacaaa
      181 aagccggagt cagtcagtat ccattaacac ccggttcgct ttatgttggc gtgtacggcg
      241 gcagcccggc agatatcgcg gcggccatct ggcgtaaggc tccgccgggt attgatatga
      301 atggcaacac aacgttcact gttgcagata aggagtacga tccgccgtat cctgaatacg
      361 tgatcagctg gcagacactc aaacccgtca gtctgcatgt cagtgtgacg ctgaaaaaaa
      421 gtgactacct gccttcagat attacccgac aggtacagca atccgtgctg gacgcattta
      481 atggtacaga tggtggtctg cgggcaagag ttgcctctgt tgtctctgcc gggcgctact
      541 atgccggcat ttacaaaacc gatccggaac atattgatat tctgggcctt actgtgagcc
      601 gtgatggttc gtcatggaca acggctgtca ctttcgggat agatgagatt ccggttctgg
      661 atgtgtcgga tatcagtgtg aaactccagg aggcataacg tacagaatgt ggctgccact
      721 gtgcttgcgc agtatgctgc cagcccccga ctcaatgccc tcattaacag ctttaacgca
      781 gcgctttccc ccgacagttt tatcagtgat ttttatggtc ttatctggaa catcgatacc
      841 gcagaaaagt atggtcttga tgtctgggga aagattgtgg gtgtcagttg ctggctgacg
      901 gtaaaggacg attttaatta cctgggcttc agcgagtcca ggatggacac cccggtaatg
      961 gatgatccct gtccgtttaa tcaggcaccg ttttacaacg gaaaatcgga tacccggact
     1021 gttgacctgt ctgatgctgt ataccggcgg ctgatactga tgaaagccat gtcgaacatt
     1081 actgactgtt ccgttccgga tattaacctg atgctgagat ttatgttcgg aaaaaaacgc
     1141 cgggcttatg ttctgaataa tggcgggctg aggatgagtt acgtctttga gtccgcgctc
     1201 tcgttggcag aactggcgat tatccagtcg tcgggtgcac tgccatcccc gccgggtgtt
     1261 tatgtttcag tagttttaaa ggagtcccgt aatgaagg
//
LOCUS       DAJTQY010000244         1272 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.252, whole genome shotgun sequence.
ACCESSION   DAJTQY010000244 DAJTQY010000000
VERSION     DAJTQY010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1272
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.252"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            <1..182
                     /locus_tag="OO349_004108"
     CDS             <1..182
                     /locus_tag="OO349_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5868966.1"
                     /translation="STIKKGDPVYVVVSTDESIKVPLGGFMSTSVSGKNVVLTNAEFT
                     GAGDANGNAEISWKI"
     gene            194..1135
                     /locus_tag="OO349_004109"
     CDS             194..1135
                     /locus_tag="OO349_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2184 domain-containing protein"
                     /protein_id="HCR5868967.1"
                     /translation="MITFDQATVDSSGAFLIGELERLDQTLNLPLAGYTWTRDIQLRE
                     DVSIADDISSWTNTSFAAAGTGANPNGKNWVGKDSTAIAGVNVDIGKSGNPLNLWGME
                     LGWTVVELAAAQQVGRPIDTQKYDGMQLKWQMDNDEQVYVGDSALNLKGLVTLDGVPV
                     NNAAKTWAISTPDEIRASINQVLSDAWAASGYSVVPRDLLIPPEQFALLSSIIVSSAG
                     NQSLLTYLQTNTISYHQNGVPLNIRAVKWLKGRGVGNKDRMVAYTNDKKYVRYPLVPL
                     QSVPIQYRGLYQIATYYGKLGAVEPVYKETISYVDGI"
BASE COUNT          309 a          332 c          347 g          284 t
ORIGIN      
        1 ccagcaccat caaaaaaggc gacccggtat acgttgtcgt ctccactgat gaatccatca
       61 aagtgccgct gggtggattc atgtccacgt cagtcagtgg caaaaatgtg gtgctgacca
      121 acgctgaatt cacaggtgcc ggtgatgcta acggcaatgc agaaatttcc tggaagattt
      181 aaggaacaga cgaatgatta cttttgatca ggcaaccgtt gacagctctg gtgcctttct
      241 catcggagag ctggagcgac tcgaccagac tctgaacctg ccactggcgg ggtacacctg
      301 gacccgcgat attcagttgc gtgaagatgt ctctatcgca gatgacattt ccagctggac
      361 gaataccagc ttcgccgctg cgggtactgg tgcaaatccg aatggcaaaa actgggtagg
      421 caaagactca accgctattg ctggcgtaaa cgtggatatc ggcaaatccg gtaacccgct
      481 gaatctctgg ggcatggaac tgggctggac cgttgtagag ctggcagcag ctcagcaggt
      541 aggtcgcccg attgacaccc agaagtacga cgggatgcag ctcaaatggc agatggacaa
      601 cgacgagcag gtgtatgttg gcgattccgc attaaacctg aaaggccttg ttaccctgga
      661 cggcgtgcct gtcaacaacg ctgccaaaac gtgggcaatc tcaacaccgg acgaaatccg
      721 cgcaagcatt aaccaggtgc tgtctgatgc gtgggccgct tccggttact ctgtggttcc
      781 gcgtgatttg ctgatcccgc cagagcagtt tgctctgttg tccagcatca tcgtttcatc
      841 tgcgggtaac cagtccctgt tgacgtacct tcagaccaac accatcagct atcaccagaa
      901 cggtgttccg ctgaatatcc gcgcggttaa atggctgaaa ggccgtggtg tggggaataa
      961 ggatcgcatg gttgcgtaca ccaacgataa aaaatacgtc cgctatccgc tggtgccgtt
     1021 gcagagcgtt cctatccagt atcgcggtct gtatcagatt gcgacctact acggcaagct
     1081 gggtgcggtt gagccagtgt acaaagaaac catttcgtac gttgatggca tttaacagcc
     1141 acatggcccc cctggcgggg ccattaagga tgacccgatg gcaaaaaata atgcagtaat
     1201 acacgtacat accccgtttg tgctcacgct tcccgacggt tcgcggcgcg agtttgttaa
     1261 aggccgtcat gc
//
LOCUS       DAJTQY010000245         1270 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.253, whole genome shotgun sequence.
ACCESSION   DAJTQY010000245 DAJTQY010000000
VERSION     DAJTQY010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1270
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.253"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            206..421
                     /locus_tag="OO349_004110"
     CDS             206..421
                     /locus_tag="OO349_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5868968.1"
                     /translation="MMNIEELRKIFCEDGLYAVCVENGNIVSHYRIVCLQKNGAALIN
                     FVDARVTDGFILRDGEFVTSLQALKEI"
     gene            524..802
                     /locus_tag="OO349_004111"
                     /pseudo
     CDS             524..802
                     /locus_tag="OO349_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            804..>1270
                     /locus_tag="OO349_004112"
     CDS             804..>1270
                     /locus_tag="OO349_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5868969.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESMPVFHNTRVLVEPEPKSMRN
                     LPSGVVPAVRQPLVEDKTLLPFFSNARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGT"
BASE COUNT          313 a          318 c          322 g          317 t
ORIGIN      
        1 gtgagaaatc cctcgccacc tctgatgtgg caggcatcct caacgcaccc gcacttaacc
       61 cgcttcggcg ggtttttgtt tttattttca acgcatttga agttctggat ggtgccggaa
      121 tagaatcaaa aatacttaag tagcgcgcag ggataaaagg gatggacccc gaacagggga
      181 gtgctactta tctggaagga ttctgatgat gaacatcgaa gaactgcgta aaattttttg
      241 tgaagatggc ctttatgctg tgtgcgttga aaatggaaat attgttagtc attaccgcat
      301 tgtgtgtttg caaaaaaatg gggctgcgtt aattaatttt gtggatgccc gagtgacgga
      361 cggatttatc ttgcgcgacg gtgagtttgt cacttcatta caggcattga aagagatctg
      421 aataaaagct ggcttttctg ctttttcaga agaataaact catctacaat cttgcgcggg
      481 gctgaactcc cgctgagtaa caccgtgcca ccggagaaaa ccgatggcac gcaacgcaaa
      541 atattacaat catgataatt cgaccgttct tgcccacacg cacgagcggt attctcacgc
      601 atttaagtca gactggtacc agcatccctc atgcactgaa gaacaggccg aatggcttat
      661 tcagtgttac cgcaggcgcg gatgcgaggt taaaaaagcc cttagcctcg actaccgtca
      721 ctagataatc tccgtcaggc tcccttactc cgaacagcca ccgcgtccgt cccgcacatt
      781 ccagcaacgg atctggaggt aacgtgcggg tattacttcg acctgttctg gttccggaac
      841 tcgggctggt ggtccttaag cccggtcgtg aatccatgcc ggtattccac aatacccggg
      901 tactggtgga gccggaaccg aaaagcatgc gtaatctgcc gtccggggtc gttcctgccg
      961 ttcgccagcc gctagtggaa gacaaaacat tgctgccgtt tttcagtaac gcacgggtga
     1021 ttcgtgctgc tggtggtgct ggcgcattgt ctgactggct gttgcgccat attaaatcct
     1081 gccagtggcc acacggcgat tatcatcaca gcgaaaccgt cattcaccgt tatggtaccg
     1141 gcgcaatggt attgtgctgg cactgcgaca accagctgcg tgaccagaca tccgaatcac
     1201 tcgagcaact tgctcatcaa aacctgtcag catggatgat tgacgtcatc ggtcacgcaa
     1261 taagcggtac
//
LOCUS       DAJTQY010000246         1259 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.254, whole genome shotgun sequence.
ACCESSION   DAJTQY010000246 DAJTQY010000000
VERSION     DAJTQY010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1259
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.254"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            34..435
                     /locus_tag="OO349_004113"
     CDS             34..435
                     /locus_tag="OO349_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HCR5868970.1"
                     /translation="MAKKDDNLKRLRELAASLGREPDISGSAADIAQRVAELEEELAN
                     MDDTDIRDKSAHPENALTGHENEVISAQPETVIQNMDDLVTVVALVTLHTDALHATRD
                     EPLAFVPPGMAFRVFAGVAAGMTERGLARMQ"
     gene            447..818
                     /locus_tag="OO349_004114"
     CDS             447..818
                     /locus_tag="OO349_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5868971.1"
                     /translation="MADFDNLFDVALDLADKAIIRNMGIRAVITSGRLKGIMISGIFD
                     DPENISLVAGGVRIEDSLPSLFVKTADILRLCRNDSLMIGRESFCVDRITPDDGGCSY
                     IRLRREGLPGNVRAGRYYEGA"
     gene            805..>1259
                     /locus_tag="OO349_004115"
     CDS             805..>1259
                     /locus_tag="OO349_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5868972.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQASAWAINRVAQKAVSVATRQVAG
                     NTVAGDNQVKGIPLKLVRQRVRVFKASPSGKMTARIRVNRGNLPAIKLNTTRRRAGEG
                     LRVGKYFFRGAFVQQLANGRWHVLRCLPEARFATGHDHQGRLRKNRLPV"
BASE COUNT          299 a          281 c          390 g          289 t
ORIGIN      
        1 gggctcccgt tttttattcg gacaggaaca gatatggcaa agaaagacga taacctgaaa
       61 cgtctgagag aactggcagc atcgctggga cgtgagccgg atatctcggg aagcgcagca
      121 gatattgcgc agcgtgtggc tgagctggag gaggaacttg ccaatatgga tgacactgac
      181 atccgggata agtctgccca cccggaaaat gcgctgaccg gacatgaaaa tgaggtgata
      241 tcagcgcagc cggagaccgt gattcagaat atggatgatc tggttacagt cgtggcactg
      301 gtgacgctgc ataccgatgc acttcatgcc acgcgggatg aacctctggc atttgtgccg
      361 ccgggaatgg cgttccgtgt ctttgccggt gtggcagccg gaatgacaga acgtggcctg
      421 gccagaatgc aataacggga ggcagcgtgg ctgatttcga taatctgttt gatgttgcac
      481 ttgatcttgc agacaaggcc attattcgca atatggggat tagagcggtc attacgtcag
      541 gccggctaaa agggatcatg atttccggga tttttgatga tcctgaaaat atttctctgg
      601 tggccggcgg tgtgcgtatt gaagactctt taccatccct gtttgtgaaa acagcagata
      661 ttttacggct gtgtcgcaat gattcgctga tgattggtcg tgagtctttc tgtgtggatc
      721 gtatcacccc tgatgatggc ggatgtagtt atatccggtt gcggcgtgag gggctgccgg
      781 gaaacgtaag ggcaggacga tattatgaag gggcttgaga atgccatccg caatctgaac
      841 agccttgata cccgtatggt gccacaggcc agcgcatggg cgataaaccg tgtggcacag
      901 aaagcggtct cggttgccac ccggcaggtt gccgggaata ccgttgcggg cgataaccag
      961 gtgaaaggga tccccctgaa actggtacgt cagcgtgtcc gggtgtttaa agccagtccg
     1021 tcaggaaaaa tgacggccag gatccgcgtt aaccggggca atctgcccgc catcaaactg
     1081 aacacaacac ggcggcgtgc tggtgaagga ctgagagtgg gaaaatactt tttccggggg
     1141 gcatttgttc agcaactggc gaatggccgc tggcatgttc tgaggtgtct tcctgaagcg
     1201 cgttttgcaa cagggcatga ccatcagggc aggctaagaa aaaatcgtct tcctgtgga
//
LOCUS       DAJTQY010000247         1252 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.255, whole genome shotgun sequence.
ACCESSION   DAJTQY010000247 DAJTQY010000000
VERSION     DAJTQY010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1252
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.255"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            209..>1252
                     /locus_tag="OO349_004116"
     CDS             209..>1252
                     /locus_tag="OO349_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR5868973.1"
                     /translation="MTESSDYESVQVFIGVDVGKDTHHAVAINRSGKRLFDKALPNDE
                     NKLRSLISDLKQHGQILLVVDQPATIGALPVAVARSEGVLVGYLPGLAMRRIADLHAG
                     EAKTDARDAAIIAEAARTLPHALRTLKLADEQIAELSMLCGFDDDLAAQTTQASNRIR
                     GLLTQIHPAPERVLGPRLEHPAVLDLLQRYPSPEKLASLGEKKLAAQLCKLAPRLGKR
                     LAADIAQALAEQTVVVPGTNAAAVVLPRLALQLITLRKQRDEVALEVEQRVLAHPLYP
                     VLTSMPGVGVRTAARLLTEVACRAFASAAHLAAYAGLAPVTRRSGSSIRGEHPSRRGN
                     KALKRALFLSAFAA"
BASE COUNT          260 a          390 c          337 g          265 t
ORIGIN      
        1 ttataatggg gtgttagcca gccagacccg gcatgattac tgcccccagt cgtccatgat
       61 ccgggggggg atgtcaccgg gtctggtggg gcgctggtaa ccgctaatag gggtcaggtc
      121 aggcactttt gccgggaccg tctgtaacgt ggatgccggt acctgctccc cgtggttatc
      181 tggttaaccc atatacaagg gagacagaat gaccgaatcc agcgattacg aatccgtcca
      241 ggtctttatc ggcgttgatg tcggtaaaga tacgcatcac gctgttgcca ttaatcgttc
      301 aggtaaacgc ctgttcgata aagcattacc caacgacgaa aacaaactca ggtcgctaat
      361 atctgacctg aaacaacatg gtcagatact gctggttgtt gatcagccag ctaccatcgg
      421 tgcgttacct gtcgccgttg cccgctcaga aggagtcctt gtcggatacc tccctggact
      481 ggccatgcgc cgcatagccg acttacacgc cggtgaagct aaaactgatg ctcgtgacgc
      541 tgccatcatt gccgaagctg cccgtaccct gcctcacgcg ctacgcacgc tgaaactggc
      601 tgacgagcaa atcgccgaac tctccatgct ctgcggcttc gatgatgatc ttgccgcaca
      661 gacaacgcag gccagcaacc gtatccgcgg ccttctgacc cagatacatc cggcaccgga
      721 gcgcgttctc ggtccgagac ttgagcaccc ggcggtactc gatcttctcc agcgatatcc
      781 ctcaccagaa aaactcgctt cgctgggtga gaagaagctg gcagcccagc tctgcaaact
      841 tgcgcctcgt ctgggtaaac gccttgcagc agacatagct caggcactgg ccgaacaaac
      901 cgtcgtcgtt cccggcacga atgccgctgc cgtagtactg ccacgtctgg cactccagct
      961 catcacgctg cgtaagcaaa gagacgaggt ggcgcttgag gtagaacagc gagttcttgc
     1021 tcaccctctt tacccggtcc tgaccagtat gcccggagtc ggtgtcagga ccgcagccag
     1081 actcctcacc gaggtcgcct gccgcgcctt cgcctctgcc gcacatctcg ctgcttatgc
     1141 tggccttgcg ccggtaactc ggcgatccgg ctcgtcaata cgcggtgagc atccctcgcg
     1201 acggggtaat aaagctctca aacgggcgtt gttcctgtcg gccttcgccg cg
//
LOCUS       DAJTQY010000248         1200 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.256, whole genome shotgun sequence.
ACCESSION   DAJTQY010000248 DAJTQY010000000
VERSION     DAJTQY010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1200
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.256"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(183..620)
                     /locus_tag="OO349_004117"
                     /pseudo
     CDS             complement(183..620)
                     /locus_tag="OO349_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001382442.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1125..>1200)
                     /locus_tag="OO349_004118"
     CDS             complement(1125..>1200)
                     /locus_tag="OO349_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCR5868974.1"
                     /translation="LSFSKSVELHDKVIGHYLNIKHYQ"
BASE COUNT          239 a          318 c          290 g          353 t
ORIGIN      
        1 ttgctgactc cccgattgtt gatgagcgcc ggacctcgcg caacgctttc ccagaggcag
       61 ttttgccttc cccgagcggt cacgcaaaac gttgcaacga cctgatcatt tgcagttttt
      121 agcggaacgc agatataaaa aatccccggc tttttgtgcc agggacgttc atagctgaaa
      181 atttatttta cgttttcggc gacgggagtt gttgagatta agggtgaagt caatgccgcc
      241 agtgaaccgc ctgaaacgcc aatttcattg agcaaggagt caatcagcgg agcctgtgtg
      301 cggtaagaga gagcggctga taatgcttgt tccgctaggt ttccgccctc cacattaccc
      361 gtgtttgcat cacccgcagc gctgccacta ttcagaccat cgacctaaat aatcttgata
      421 ccgtcaatag atttcatcgg ctcaacggat ttttctatta ccgcaggtag cgcctgcaac
      481 aaggccagtt tgaatttaag gctggtttgt tcatcagaaa gtacgttgat agcatcgttc
      541 agcgcacgtt gtgcttctgc ttccgccaga ccctttttac gtgtagcttc ggctaactca
      601 acgatagccg ccgcctgcat ctctgcggct tctttttctg ctttcgcccg cacggtcagt
      661 tcaaccgctt tggtttctgc atcctgcgcg gcagcgatta gggcaacttg tttagcacga
      721 tcggcttctg cggtctggcg agtggtttct acgttttgtt gcgcacttac tgcttctgcg
      781 agtgcaaggt tagcacgcgc ttctgcctgg gactgttgtt ccgatttggc ggcaatagcg
      841 atcgatttcg tctggttggc gatttcggtg acctgctgct gttcgatctc tttaatacga
      901 acttcacgtt cagcctcaac ctttcttgag cggacggcct gctcgcgctc gatctccgtt
      961 tcctgaattt gtcgttcagc cagaattcgc gtctgctctg cttcacgacg acgttcagct
     1021 tcaaaagccg caatacgtgc attctgttcg gcagtacggg ttttaacctg ctgctcctgc
     1081 tcaagcgtca taaacgcttc ttgttgttcg gtaatgactc caacttactg atagtgtttt
     1141 atgttcagat aatgcccgat gaccttgtca tgcaactcca ccgattttga gaacgacagt
//
LOCUS       DAJTQY010000249         1131 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.257, whole genome shotgun sequence.
ACCESSION   DAJTQY010000249 DAJTQY010000000
VERSION     DAJTQY010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1131
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.257"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            <1..312
                     /locus_tag="OO349_004119"
     CDS             <1..312
                     /locus_tag="OO349_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5868975.1"
                     /translation="EPHRPFRVSCEEGEFVVYENGKPVRASHHHCLKIINLAIRCLKD
                     ENTRVMKRIGRCMGYLQVAAEIEALASGADMDAAVREALLRDFNTPPLRKSLMTGSSR
                     G"
     gene            378..911
                     /locus_tag="OO349_004120"
     CDS             378..911
                     /locus_tag="OO349_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706619.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HCR5868976.1"
                     /translation="MSAKIKYGLSAAVLALIAAGASAPQILDQFLNEKEGNHTTAYRD
                     GSGIWTICRGATMVDGKPVIPGMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDVGRDCRIRSNNCYGQ
                     VIRRDQESALACWGIDQ"
     gene            908..>1131
                     /locus_tag="OO349_004121"
     CDS             908..>1131
                     /locus_tag="OO349_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HCR5868977.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAITYKEQRDKNAGEL
                     KLANATITDMQQRQRDADALDAKYTKELADA"
BASE COUNT          291 a          258 c          310 g          272 t
ORIGIN      
        1 gaaccccatc ggcctttcag ggtttcctgt gaagagggtg agttcgtcgt ttatgagaac
       61 ggaaaacctg tccgggcatc gcatcatcac tgcctgaaaa ttattaatct cgctatcaga
      121 tgcctgaaag acgaaaacac cagagttatg aaacgcattg gccgttgcat ggggtatttg
      181 caggtggcgg cagagattga agcacttgcc agtggtgcag acatggatgc tgcggtgcgg
      241 gaggctcttc ttcgtgattt caatactccc cctttaagaa agagcctgat gactggatcc
      301 agccggggct gacttatctt aaaaggcgta tataagttgg ctcgttattt gttgccgata
      361 aatcctgata aatatccatg agcgcaaaaa tcaaatacgg cctgtcggct gctgttctgg
      421 cgctgattgc cgcaggcgcg tctgctcccc agatacttga ccagtttctg aacgaaaaag
      481 agggtaatca cacaacggca taccgtgatg gttctggcat atggaccatc tgtcgtggtg
      541 ccacaatggt ggatggtaag cctgtcatac cgggaatgaa gctgtcgaag gaaaaatgtg
      601 accaggttaa cgctattgaa cgtgataagg cgctggcatg ggtggagcgc aatattaaag
      661 tacctctgac cgaaccccag aaagcgggta tagcgtcatt ttgcccctat aacattggcc
      721 ccggtaagtg tttcccgtcg acgttttata agcggctgaa tgccggtgat cgtaagggcg
      781 catgcgaggc gattcgctgg tggataaaag atgttgggcg cgattgccgc atacgttcaa
      841 ataactgcta tggacaggtt attcgtcgtg accaggaaag cgcattagcc tgttggggga
      901 tagatcaatg agcagagtca ccgcgattat ctccgctctg gttatctgca tcatcgtctg
      961 cctgtcatgg gctgttaatc attaccgtga taacgccatt acctacaaag agcagcgcga
     1021 caaaaacgcc ggagaactga agctggcgaa cgcaaccatt actgacatgc agcagcgcca
     1081 gcgtgatgct gatgcactcg atgctaaata cacgaaggag ttagctgatg c
//
LOCUS       DAJTQY010000250         1111 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.258, whole genome shotgun sequence.
ACCESSION   DAJTQY010000250 DAJTQY010000000
VERSION     DAJTQY010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1111
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.258"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            1..1019
                     /locus_tag="OO349_004122"
                     /pseudo
     CDS             1..1019
                     /locus_tag="OO349_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
BASE COUNT          312 a          272 c          259 g          268 t
ORIGIN      
        1 aattctcttg caccattaac tatgttcagt ccctgcgcga gttgtacacc gatgtcatcg
       61 aagagtttta ctgggttgcg ctcccgctta cgacgcaaaa ttccctttca caataccaac
      121 ccgaatggca gtgctgggaa cctgatgtcg aatgggtgcg tcagcccccg caagatgcga
      181 taaccgctcc tgactttttc tgcttttacc agccaggcat gaccttcgaa caatttgtac
      241 gtgagtttgc cgaatggttt tcacaaaaac gtccggcggc gatgatgatc ggcatccgtg
      301 cggatgagtc ctacaaccgt tttgtcgcca tcgccagttt aaataaacaa cgttttgccg
      361 acgataaacc atggactacg gccgcaccag gcggtcatag ctggtacatt taccccattt
      421 acgactggaa agtggctgat atctggacct gatatgcaaa tcatcaaaat ctctgcaacc
      481 cactgtataa cctaatgtat caggcaggcg ttcctctgcg tcatatgcga atttgcgaac
      541 cttttggccc ggagcaacga caaggattat ggctctgtca cgttatcgaa ccggatcgct
      601 gggctgctat gtgcgcacga gtcagcgggg taaaaagtgg cggcatttac gccggacatg
      661 acaatcattt ctatgggcat cggaaaatcc tcaaaccaga acatttagac tggcaagaat
      721 atgcattatt gctgctcaat agcatgccgg aaaaaacagc tgagcattac cgcaataaaa
      781 tcgccattta tttgcactgg tatcagaaaa aaggcatcga ggttccacaa acccagcaag
      841 gggacattgg cgcgaaagat atcccctcct ggcggcggat atgcaaagtt ttactcaata
      901 acgattactg gtgtcgggca ttatcattta gtcctacgaa agcgaagaac tatcagcgtt
      961 ataacgaacg gataaaagga aaacgtcagg aatgggggat actatgcaac aacgattaac
     1021 gcaggattta acgcaatttc tcgccagctt gccggaagat gatcgcatca aagcgattaa
     1081 tgaaatccgc atggcgatcc atcaggttag c
//
LOCUS       DAJTQY010000251         1079 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.261, whole genome shotgun sequence.
ACCESSION   DAJTQY010000251 DAJTQY010000000
VERSION     DAJTQY010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1079
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.261"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(54..130)
                     /locus_tag="OO349_004123"
     tRNA            complement(54..130)
                     /locus_tag="OO349_004123"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(94..96),aa:Arg,seq:tct)
     gene            complement(132..207)
                     /locus_tag="OO349_004124"
     tRNA            complement(132..207)
                     /locus_tag="OO349_004124"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(172..174),aa:Ile,seq:cat)
     gene            complement(384..1073)
                     /locus_tag="OO349_004125"
     CDS             complement(384..1073)
                     /locus_tag="OO349_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HCR5868978.1"
                     /translation="MNNQYLQFVREQLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGASIPLVSQVAFSTSSWRRAVLSLEEHYK
                     AWLLWCYSGSICWEYQITITHWAWEEFKAHSGTRKIAEKTQERLKKLIWLAAQAVKAE
                     LFGGEGYEYQELALLAGVTTKNWSKTFTGHWVVMKHIFHRLDSEALLFVMRTRSEQKA
                     AFSKQSIAKVD"
BASE COUNT          260 a          295 c          225 g          299 t
ORIGIN      
        1 ggtgacctac tgattacaag tcagttgctc tgcctgctga gctaagtcgg cgctggccca
       61 ccaccgagga ctcgaacctc gcaccgtcaa cttagaaggt tgatgctcta tccggatgag
      121 ctagtggtgg ttggtggccc ttgctggact tgaaccagcg acctggcgat tatgagtcgc
      181 tcgctctcac cactgagcta aagggccgga ggcagaataa taaccatatg tcatcacatc
      241 tgcaaactca tctgaccacc agcacgttta acgtcctgtg ccatttttca ggcataaaaa
      301 aaccgctcgg cggcgggttt tgcttacttt accatcgcgt acaaaatcgg caaagtatca
      361 gatttgcatg agatatatgc cttttaatct acttttgcaa tactttgctt tgaaaatgcc
      421 gctttttgtt ctgaacgtgt tctcatcaca aacaataaag cctcactatc cagtcggtga
      481 aaaatgtgtt tcattacaac ccagtgacca gtaaatgttt tggaccagtt tttggttgtt
      541 actcccgcca gtaatgccag ctcctgatat tcataacctt ccccaccaaa aagttctgct
      601 tttaccgcct gcgccgccag ccagattaat tttttcaggc gttcctgtgt tttctctgca
      661 attttcctag tgccagaatg agccttaaac tcttcccacg cccagtgggt tatggtgatc
      721 tgatactccc agcaaatact tccgctgtaa caccacaaca accaggcttt atagtgctct
      781 tcaagagaca gaacagcccg ccgccacgat gatgtcgaaa acgcaacctg actgaccaga
      841 ggaattgacg cccccttcgc cagtgattgt tttcccggga ttggtggatt atcccgcgtt
      901 atcatttttc cggtcacctc atcgcggtac cggatttttt tacgcctgta acgccctgta
      961 tcgaacatgg cattctcctg ccaggcttca agctgacctt ttgtcgcccc actcaaatca
     1021 gcggtggcga taatgagctg ctcacgaaca aactgtaaat actggttatt catgcacac
//
LOCUS       DAJTQY010000252         1031 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.262, whole genome shotgun sequence.
ACCESSION   DAJTQY010000252 DAJTQY010000000
VERSION     DAJTQY010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1031
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.262"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            154..477
                     /locus_tag="OO349_004126"
     CDS             154..477
                     /locus_tag="OO349_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HCR5868979.1"
                     /translation="MLLKEEEIKSHLRLDDGLYSDGDFLKLLAQAVQKKTETYLNRKL
                     YAPEETIPEDDPDGMHLTDDVRLAMLMLVSHFYENRSTITDVEKLETPMSFRWLAGPY
                     RIVPL"
     gene            474..890
                     /locus_tag="OO349_004127"
     CDS             474..890
                     /locus_tag="OO349_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HCR5868980.1"
                     /translation="MKIRQSQTSATYLLPDPGELNRRIKIRLRVDEPTADFGTEPTYP
                     ESFDVWAKVAQPGAAAYQGSVQTENIVTHYFTIRFRHDITADHEVVYYGQEYRIRRIR
                     DLNGQRRFLLLECEELRTARRRGECHESDSIFTRRL"
     gene            856..>1031
                     /locus_tag="OO349_004128"
     CDS             856..>1031
                     /locus_tag="OO349_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5868981.1"
                     /translation="MNQTAFLHVDFKQPEELEFNRARLRRAFVQIGRVYMRDARRLVI
                     KRGRSGPGENPGYQT"
BASE COUNT          262 a          237 c          293 g          239 t
ORIGIN      
        1 gttcgtgggt ttttatacca ccaagcgaac cggcggtatg ctggtggatt ctcaggcgat
       61 taagctgatg aaaattgggg ctgcaacccg ccagaaagcc gctgcgtaat gcggtttttt
      121 gtgcccgcgt aatcgcgggc acaggaggaa aatatgctcc tgaaagaaga ggagattaaa
      181 tctcatctcc ggctcgatga tggtttgtac agtgacggcg attttctgaa actgctggca
      241 caggcggtac agaaaaaaac agagacatac ctgaacagga agttgtatgc accggaagag
      301 acgattccgg aagatgatcc tgacgggatg catctgactg atgacgttcg tctggcaatg
      361 ctgatgctgg tcagtcattt ttatgaaaat cgctcaacga ttaccgatgt ggagaaactg
      421 gaaacgccaa tgagtttcag atggcttgct ggcccttaca ggattgtccc gctatgaaaa
      481 tcaggcaaag tcagaccagt gccacttacc ttttaccgga tcccggagag ctgaatcgcc
      541 ggataaagat ccgccttcgt gtggatgaac ccaccgctga ttttggtacg gagccaacgt
      601 atccggagtc gtttgatgtc tgggcaaagg tggctcagcc aggcgctgcc gcttatcagg
      661 gctcagtgca gacagaaaat atcgttacgc attattttac gatccgtttc cggcacgaca
      721 tcacggcaga tcacgaagtg gtttattacg gccaagagta ccggatccgg cgaatacgtg
      781 acctgaacgg tcagcggcgt tttttattac tggaatgtga agaactgcgt acagcgcgac
      841 gacggggtga atgccatgaa tcagacagca tttttacacg tcgactttaa acaaccagag
      901 gagctggagt ttaatcgcgc ccgtctgcgt cgggcgtttg tgcaaatcgg gcgcgtttac
      961 atgcgtgatg ccagacgtct ggtgattaaa cgcggacgct ccggtccggg agagaacccg
     1021 ggatatcaga c
//
LOCUS       DAJTQY010000253          983 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.263, whole genome shotgun sequence.
ACCESSION   DAJTQY010000253 DAJTQY010000000
VERSION     DAJTQY010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..983
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.263"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(20..823)
                     /gene="dkgB"
                     /locus_tag="OO349_004129"
     CDS             complement(20..823)
                     /gene="dkgB"
                     /locus_tag="OO349_004129"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HCR5868982.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNAIPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
BASE COUNT          259 a          220 c          222 g          282 t
ORIGIN      
        1 gaggagctgt cagagaggct taatcccatt caggagccag accttccggg ctaaccaggc
       61 ggtcgttgca atccagtgcg gcgatcgctt ttttatcttc ggcatcaagc tgtaaatttt
      121 gtgccttaag attactttcc aggtttttac gtttagtaga agaaggaatt actgagtaac
      181 cttcccccat agcccacgcc agaatcactt gtgccggaat cgcattgtgt ttagctgcaa
      241 tacgagcaat aacctcatct ttcagtgcct taccataagc cagcgtcata taggaagtga
      301 tatgaatacc gtgctgttta gcccaggcaa ccactttacg gttttgcaga taaggagaga
      361 gttcaatctg gttggtagcg atgttttcag cgccaacagc agcaatcgct ttttccatca
      421 atgggatcgt gaagttggaa ataccgatct cacgcgtcag cccttgtttt ttggcttcca
      481 gcagcgcctg cataaactct tcaacagaga cttcatcgtt tggtgacggc cagtggatta
      541 gcgtcagatc aacataatcg gtacgcaatt tttgcaggct ctctttcaga ctcgggatca
      601 atttgtcttt gctgagattt tcaatccaga ttttagtggt gatgtagagt tcatgacgtg
      661 gcacgccact ttctgcaatc gcctgaccta ctgcggcttc gttatcatag atttgtgcgg
      721 tatcaattgc gcgataacca agttcaagcg ccgttttcac agatgaaata acaacgtcgt
      781 ctttcagacg gaaagtacct aaaccaaatg cagggatagc cataatatgc ctcttttaat
      841 gctattttcg ttaactgagg tgattatggg cttcagactg ttgcggaaaa agagcgtaaa
      901 acgcagagga tttttgcgat tctagcaata atagatatcc agacgcaaaa aaacatcgag
      961 cgttaactcg aggcttctta ata
//
LOCUS       DAJTQY010000254          968 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.264, whole genome shotgun sequence.
ACCESSION   DAJTQY010000254 DAJTQY010000000
VERSION     DAJTQY010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..968
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.264"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(<1..171)
                     /locus_tag="OO349_004130"
     CDS             complement(<1..171)
                     /locus_tag="OO349_004130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5868983.1"
                     /translation="MALPFAADACLVASSFEADADDADDAAEPADVLAARAEPAASVA
                     LVAASLADVPASL"
     gene            257..968
                     /locus_tag="OO349_004131"
                     /pseudo
     CDS             257..968
                     /locus_tag="OO349_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459840.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
BASE COUNT          251 a          257 c          288 g          172 t
ORIGIN      
        1 cagggatgcc ggcacatctg ccagtgaggc ggcaaccaat gcgactgatg ctgcaggctc
       61 agcacgcgca gccagcacgt cagccggttc ggcagcgtca tcggcatcat ctgcgtctgc
      121 ttcaaaagat gaggcgacta aacaggcgtc agcagcgaag ggcagtgcca cgacggcatc
      181 ctctaaggcg acagagtctg caggcagtgc gacggcggca gcgcagagca aaagcgcggc
      241 agaatccgcg gcaatgcgcg ctgagacagc agcaaaacgg gcagaggata ttgctgatgt
      301 cgtatctctg gaagatgcga gcacgacgaa aaaggggata gtgcagctca gcagtgcgac
      361 caacagtgca tccgagtcac tggccgcgac agcgaaagcg gttaaggtgg taatggatga
      421 aacaaacaag aaagcgcccc tgaacagtcc ggctctgacc ggaacgccaa ccacacccac
      481 tgcgccaaag gggactaata atactcagat cgccagtacg gcttatgtta tggccgcgat
      541 tgctgccctc gtggactcgt cgcctgatgc actgaacacg ctgaatgaac tggcggcggc
      601 gctgggcaac gacccgaatt ttgcgaccac catgactaac gcgcttgcgg gtaagcaacc
      661 gaaagatgcc actctgacgg cgctggcggg gcttgctact gcggcagaca ggtttccgta
      721 ttttacgggg aatgatgtcg ccagcctggc aaccctgaca aaagtcgggc gggatattct
      781 tgcgaaatcg accgttgccg ccgttatcga atacctcggt ttacaggaaa cggtaaacaa
      841 ggctgataac gctgtccaaa agacaggcga taccttgtcc ggtggactta cttttgaaaa
      901 cgactcaatc cttgcctgga ttcggaatac tgactgggcg aagattggat ttaaaaatga
      961 ttcggatg
//
LOCUS       DAJTQY010000255          948 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.265, whole genome shotgun sequence.
ACCESSION   DAJTQY010000255 DAJTQY010000000
VERSION     DAJTQY010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..948
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.265"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(<1..752)
                     /gene="ipaH3"
                     /locus_tag="OO349_004132"
     CDS             complement(<1..752)
                     /gene="ipaH3"
                     /locus_tag="OO349_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase IpaH3"
                     /protein_id="HCR5868984.1"
                     /translation="MSIMLPINNNFSLSQNSFYNTISGTYADYFSAWDKWEKQALPGE
                     NRNEAVSLLKECLINQFSELQLNRLNLSSLPDNLPPQITVLEITQNALISLPELPASL
                     EYLDACDNRLSTLPELPASLKHLDVDNNQLTMLPELPALLEYINADNNQLTMLPELPT
                     SLEVLSVRNNQLTFLPELPESLEALDVSTNLLESLPAVPVRNHHSEETEIFFRCRENR
                     ITHIPENILSLDPTCTIILEDNPLSSRIRESLS"
BASE COUNT          271 a          152 c          232 g          293 t
ORIGIN      
        1 gacagagact ccctgatccg tgaggacaga ggattgtctt cgaggatgat agtgcaggtc
       61 ggatcaaggc taagtatatt ttccggaatg tgtgtgatgc gattctcgcg gcaccggaaa
      121 aatatctcgg tttcctctga gtgatgattt cttacaggta cggctggtag gctttccaga
      181 agattagtac ttacatcgag cgcttccagt gattcaggta actcagggag aaatgtcagc
      241 tggttatttc ttactgagag cacttccagc gatgtaggta attcaggaag catggttagc
      301 tgattgttat ctgcattaat atattccagc aatgcaggca attcaggaag catggttagt
      361 tggttgttat ctacatcaag atgtttcaga gatgcgggta attcaggaag tgttgacagg
      421 cgattgtcac aggcgtcaag gtattccagc gatgctggca attctggtaa tgatattagg
      481 gcattctgag taatttccag aacagtgatt tgaggtggta agttgtcagg tagcgaggac
      541 agatttaaac gattcagttg aagctcactg aactgattga tgagacattc tttaagtagg
      601 gagaccgctt cattccgatt ttcaccgggg agcgcttgtt tttcccattt atcccatgct
      661 gaaaagtaat cagcatatgt accggaaata gtgttataaa aagaattttg ggacaatgaa
      721 aagttattat ttatcggtaa catgatggac attcagttaa agccgatgga caaatggctt
      781 tgttaaaaga aataagtagg agtttttatg aagttcattc catgaaatga acttcagcac
      841 ttttttatta taaaaggaca aacaaaattg gctgtaaaaa aacgatactg tttcagttaa
      901 ctgtgaatag cttgcttgca ccgtattgtt cttgtttttc ggttagtt
//
LOCUS       DAJTQY010000256          884 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.266, whole genome shotgun sequence.
ACCESSION   DAJTQY010000256 DAJTQY010000000
VERSION     DAJTQY010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..884
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.266"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            440..>884
                     /locus_tag="OO349_004133"
     CDS             440..>884
                     /locus_tag="OO349_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HCR5868985.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALAFQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDD"
BASE COUNT          243 a          162 c          214 g          265 t
ORIGIN      
        1 cgtgccacgg ctggctgatg gatgttcgat agcgcgagtt tgaatgaaaa tcagccggag
       61 atgattttac ataattgcta cggaattatt caatacagga attgcttgcg tctgcatgga
      121 ttgacctgaa atattcccga aaatttctct aaaaaactcg aaaaaaatgg taactggttg
      181 aatgtattaa tatgcaatgg tacgtgtcag ggattaaaag atgaacgtaa atttattcaa
      241 cgcattaatt ttaaagggtt ttattgtttg ttgacgaaaa caggaatcat gttcggtctc
      301 tttttatctg ttaaaagcca gaagcatttc cttcgctgac tttatagtca accataacac
      361 acactctact gtctgagtcc agcgtttttt aacattcttg ttaagattat gtgatcttta
      421 gcgcgggagg aaaatattga tgaaacagcc tgcgcccgtt tatcagagaa ttgcgggtca
      481 tcaatggcga catatctggc tttctggcga tatacacggt tgtcttgagc agttgcgccg
      541 caaattatgg cattgtcgtt ttgatccgtg gcgagattta cttatctcag tgggagacgt
      601 tatcgatcgt gggccgcaaa gtttacgttg tctgcagtta ctggaacaac attgggttcg
      661 tgcggtaaga ggcaatcatg aacagatggc gatggatgcg ctggcattcc agcagatgtc
      721 tttgtggttg atgaatggcg gcgactggtt tattgcgctg gcagataatc aacagaaaca
      781 agcgaaaacg gcgctggaaa aatgtcagca tttgcccttt attcttgaag tacacagtcg
      841 caccggcaag catgttattg ctcatgccga ttatccagat gatg
//
LOCUS       DAJTQY010000257          861 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.267, whole genome shotgun sequence.
ACCESSION   DAJTQY010000257 DAJTQY010000000
VERSION     DAJTQY010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..861
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.267"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(<1..254)
                     /locus_tag="OO349_004134"
     CDS             complement(<1..254)
                     /locus_tag="OO349_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5868986.1"
                     /translation="MSLQVSHYNMLRASHEGSQKVVVRTVITVRFVPGAAIAKSILYC
                     AGQLFFKESGHHLTGTDTIYFVTVRLHMHNSTYSSIFCFS"
     gene            279..518
                     /gene="relB"
                     /locus_tag="OO349_004135"
     CDS             279..518
                     /gene="relB"
                     /locus_tag="OO349_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin RelB"
                     /protein_id="HCR5868987.1"
                     /translation="MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERL
                     PFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL"
     gene            518..805
                     /gene="relE"
                     /locus_tag="OO349_004136"
     CDS             518..805
                     /gene="relE"
                     /locus_tag="OO349_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     RelE"
                     /protein_id="HCR5868988.1"
                     /translation="MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKL
                     RGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGKRERSEVYSEAVKRIL"
BASE COUNT          239 a          174 c          219 g          229 t
ORIGIN      
        1 atgctaaagc aaaaaataga tgaataagtt gagttgtgca tatgtagcct gaccgtcaca
       61 aagtatatgg tgtctgtacc agtaagatga tggccggact ctttaaaaaa gagctgacct
      121 gcacaataca ggatggactt agcaatggct gctcctggca caaagcggac agtgatcacc
      181 gttcttacga ctactttctg acttccttcg tgacttgccc taagcatgtt gtagtgcgat
      241 acttgtaatg acatttgtaa ttacaagagg tgtaagacat gggtagcatt aacctgcgta
      301 ttgacgatga acttaaagcg cgttcttacg ccgcgcttga aaaaatgggt gtaactcctt
      361 ctgaagcgct tcgtctcatg ctcgagtata tcgctgacaa tgaacgcttg ccgttcaaac
      421 agacactcct gagtgatgaa gatgctgaac ttgtggagat agtgaaagaa cggcttcgta
      481 atcctaagcc agtacgtgtg acgctggatg aactctgatg gcgtattttc tggattttga
      541 cgagcgggca ctaaaggaat ggcgaaagct gggctcgacg gtacgtgaac agttgaaaaa
      601 gaagctggtt gaagtacttg agtcaccccg gattgaagca aacaagctcc gtggtatgcc
      661 tgattgttac aagattaagc tccggtcttc aggctatcgc cttgtatacc aggttataga
      721 cgagaaagtt gtcgttttcg tgatttctgt tgggaaaaga gaacgctcgg aagtatatag
      781 cgaggcggtc aaacgcattc tctgaaccaa agcatgacat ctctgtttcg caccgaaggt
      841 gacacttctg ctttgcgttg a
//
LOCUS       DAJTQY010000258          855 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.268, whole genome shotgun sequence.
ACCESSION   DAJTQY010000258 DAJTQY010000000
VERSION     DAJTQY010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..855
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.268"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            <1..191
                     /locus_tag="OO349_004137"
     CDS             <1..191
                     /locus_tag="OO349_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR5868989.1"
                     /translation="ENEYPQRVADRLKASGLSGDADAEREAGAQVMRETEQQIYRQLT
                     DEVLALRLSENGSQLHHS"
     gene            complement(371..>855)
                     /locus_tag="OO349_004138"
     CDS             complement(371..>855)
                     /locus_tag="OO349_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HCR5868990.1"
                     /translation="QAVPYTATADDVAPTGQQIWQELQSGKWGEIAPFTVTPEMLEAA
                     REARRQEIEAWRTEQEAKPFTFEWNGRIWNAGPDSLGRLSPVVMLAKSVTAQTHMAWS
                     DADNQQVKLSMPELEELAAAMVQAQVDRNDEIYRRQREMKEELSGLDDLASIRAFDVE
                     "
BASE COUNT          173 a          258 c          223 g          201 t
ORIGIN      
        1 tggagaatga gtaccctcag agggtggctg accggctgaa agcatcaggt ctgagcggtg
       61 atgcggatgc ggagagggaa gccggtgcac aggtgatgcg tgagactgaa cagcagattt
      121 accgtcagct gactgacgag gtactggccc tgcgattgtc tgaaaacggc tcacaactgc
      181 accattcata atcacatcgc ataaaccaca gaccggactg actccggaaa aacggaggcc
      241 cgcccccggg cctcccctga ttcatccgtt tccctgttca gcctgacagc acgccccggc
      301 tgccggatga cagactccgc ttcggtaagc aaagcggtct tctgtgattc cgccagttgc
      361 ggcttattca ttactcaacg tcaaacgccc gaattgaagc caaatcatcc agaccgctca
      421 gctcctcttt catttcacgc tgacggcgat aaatctcatc gttgcgatcg acctgcgcct
      481 gcaccattgc tgccgccagt tcttccagtt ccggcatcga cagtttcacc tgctgattat
      541 cggcatcgct ccacgccata tgtgtttgtg ctgtgacaga ttttgccagc atgactaccg
      601 gggacaggcg gcccagtgag tcggggccag cattccagat acgaccgttc cattcaaacg
      661 tgaacggctt cgcctcctgt tctgtgcgcc atgcttcaat ttcctgacgt ctggcctctc
      721 tggccgcttc cagcatttct ggtgtcacag tgaatggggc tatctcaccc catttgccac
      781 tttgcagttc ctgccagatt tgctgacccg tcggtgcgac atcatcagcg gtggctgtgt
      841 aggggactgc ctggt
//
LOCUS       DAJTQY010000259          845 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.269, whole genome shotgun sequence.
ACCESSION   DAJTQY010000259 DAJTQY010000000
VERSION     DAJTQY010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..845
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.269"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            <1..336
                     /locus_tag="OO349_004139"
     CDS             <1..336
                     /locus_tag="OO349_004139"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5868991.1"
                     /translation="YIVGSTLLIPGGVNIRGVGKASQLRAKSGLTGSVLRLSYDSDTI
                     GRYLRNIRVTGNNTCNGIDTNITAEDSVIRQVYGWVFDNVMVNEVETALFNARALALK
                     IYSMSGWNL"
     gene            563..>845
                     /locus_tag="OO349_004140"
     CDS             563..>845
                     /locus_tag="OO349_004140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="endorhamnosidase"
                     /protein_id="HCR5868992.1"
                     /translation="MEQLDLDYCGSTGVVIENVNGGFSFSNSWIAADADGTEQFTGIY
                     FRTPASTQSHKIVSGVHINTANKNTAANNQSIAIEQSAIFVFVSGCTLTG"
BASE COUNT          245 a          155 c          203 g          242 t
ORIGIN      
        1 tatattgttg gatccacttt gcttattcct ggcggtgtta acatcagagg agttggtaag
       61 gcatcgcaac tccgagcaaa aagcggactt acaggatctg tgttaaggct gtcttatgat
      121 tcagacacta tcggccgtta tctgagaaat atacgagtaa ctggtaataa cacctgcaat
      181 ggtattgaca caaacattac agcagaagac tctgtcatca gacaggttta tggctgggta
      241 tttgataatg taatggtgaa tgaagttgaa accgcattat ttaatgcaag ggctctggca
      301 ctcaaaattt atagcatgtc aggctggaac ctgtagagtc ggtcttcact ttttaggcca
      361 gtgcgtaagt gttagtgtca gctcctgcca tttcagcaga ggaaattatt ctgctgatga
      421 aagctttggc atcaggattc agcctcaaac gtatgcgtgg tcgtcagagg cagttaggtc
      481 agaagcaata attttagaca gtgagaccat gtgcattggt tttaaaaatg ccgtctatgt
      541 tcatgattgc cttgatttgc atatggaaca actggattta gattattgcg gctcaacagg
      601 cgtggttata gagaatgtaa acggaggatt ttctttctca aactcatgga tagcagcaga
      661 tgccgatggc actgaacaat ttacggggat atattttaga acgccggcct caacgcagtc
      721 acataaaatt gtcagtggtg ttcatatcaa cactgcaaac aaaaacacgg ctgcaaacaa
      781 tcaaagtata gcgatagaac agtcggcgat cttcgtcttt gtaagtggtt gtacgttaac
      841 tggtg
//
LOCUS       DAJTQY010000260          840 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.270, whole genome shotgun sequence.
ACCESSION   DAJTQY010000260 DAJTQY010000000
VERSION     DAJTQY010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..840
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.270"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(70..714)
                     /locus_tag="OO349_004141"
     CDS             complement(70..714)
                     /locus_tag="OO349_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001446249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5868993.1"
                     /translation="MTPTGTLKINAARVAARIFLMPLLVGFTREYPDIKVELTTDDSL
                     IDIVQQGFDAGVRLSGIVEKDMISVAIGPPVKLCVAAMPEYFARYGKPRHPHDLLNHQ
                     CVVFRYPSGKPFHWQFAKELEIAVAGNIILDDVDAELEAVLMGAGIGYLLYEQIKEYL
                     DTGRLECVLEDWITERPGFQVYYPNRQYMSCGLRAFLDYVKTGQICQSQRHRPQ"
BASE COUNT          230 a          196 c          195 g          219 t
ORIGIN      
        1 acgccgtttg tgctggtagg attaggactg atattcccgc tgctggcgcg taaagcgaat
       61 agcaaataat tattgtggtc ggtgacgctg actctggcag atctgacccg tttttacata
      121 atcgagaaat gcgcgcaagc cgcaggacat atactggcgg tttgggtaat agacttggaa
      181 gcctggacgc tctgtgatcc agtcttccag tacacattct aaacgaccag tatcaaggta
      241 ctccttgatc tgttcataga gcagataccc gataccagca cccatcaaaa cggcctccag
      301 ttcagcatca acatcatcca gaatgatatt tcctgcgaca gcaatttcca gctcctttgc
      361 aaattgccag tggaaaggtt tgccgctggg ataacgaaaa acaacacatt gatgattaag
      421 cagatcatga ggatggcggg gtttgccata tcttgcaaag tactctggca ttgctgctac
      481 acataatttc actggtgggc cgatcgccac gctgatcata tctttttcga cgatgccact
      541 aagtcgaacg ccagcgtcaa agccctgttg gacgatatcg ataagagaat cgtcagttgt
      601 gagctctact ttaatatcag gatattcacg tgtgaaacca accagtaaag gcattaaaaa
      661 tatacgtgca gctacccggg cggcattgat tttgagcgtt cctgtcggtg tcagacgaaa
      721 atcattcatc tcatctaaca tgatctgaat ttcgtcgaaa gctgggcgca atcgttcata
      781 gagattagaa cccgcttccg ttaacgatac gcttcgggtt gtccgattga ataagcgaat
//
LOCUS       DAJTQY010000261          835 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.271, whole genome shotgun sequence.
ACCESSION   DAJTQY010000261 DAJTQY010000000
VERSION     DAJTQY010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..835
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.271"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            148..>835
                     /locus_tag="OO349_004142"
     CDS             148..>835
                     /locus_tag="OO349_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5868994.1"
                     /translation="MYDITAAIRFAQAGGLYTYNAGFAGAIGGYAKGAILAGVATTAV
                     WLNTTDDNLTDPEGSDSAGWVNLLEDPKRIFLRQKNNLSDLQNKGTARDNLQVYSKEQ
                     SDQRYVHREGDKITGELKIRGVNALRIFNEAFGLIFRRSEECLHLIPTSEGQGENGDI
                     GPLRPFTINLRTGEISMSHKVSVGGGSQVNGALGIGVQNALGGNSIVLGDNDTGFKQN
                     GDGILDVYANS"
BASE COUNT          214 a          181 c          243 g          197 t
ORIGIN      
        1 ggagataaaa accgtatccc ggacaaggcg acacagcaga ccagagagag cggtaatgct
       61 gcgtatgatt caggttttcc tccggtgacc atgacagcgg tctcagcggg aggtataccg
      121 ccacacggca aggattttaa cggtctgatg tacgatatta ccgcagcaat acggttcgcc
      181 caggctggcg gtttgtacac gtataatgcc ggttttgcgg gggccattgg tggatatgca
      241 aaaggagcca ttctcgccgg agtcgcaaca acagcggtct ggctgaatac cacggacgat
      301 aacctgaccg atcctgaagg ctccgacagt gcgggctggg taaatcttct tgaggatccg
      361 aaaaggatat tcctgcggca gaagaacaat ctgtcagacc ttcagaataa agggacggca
      421 cgggataatc ttcaggttta cagtaaagag cagtcagatc aacgctatgt tcatcgggaa
      481 ggcgataaaa taaccggaga gctgaaaatc cgtggtgtta atgcgctgag gattttcaac
      541 gaagcttttg gcctgatttt tcgtcgttcg gaagagtgcc tgcaccttat tcccaccagt
      601 gaaggtcagg gggaaaatgg cgatattggt cccctgcgcc cgttcaccat taatttgcgg
      661 acgggtgaaa tatccatgtc gcataaagtg tctgttggcg gtggttcgca ggtcaatggt
      721 gcgctgggta tcggcgttca gaacgccctg ggggggaatt caattgttct tggtgataat
      781 gacaccggat ttaaacagaa tggagacggt attctggatg tttatgctaa tagtc
//
LOCUS       DAJTQY010000262          823 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.272, whole genome shotgun sequence.
ACCESSION   DAJTQY010000262 DAJTQY010000000
VERSION     DAJTQY010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..823
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.272"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            <1..316
                     /locus_tag="OO349_004143"
     CDS             <1..316
                     /locus_tag="OO349_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HCR5868995.1"
                     /translation="FSVVMTKDPGAACTEQFNILGSFFTTDILSDYSHLDMGNGLLLK
                     IFHKDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANPAETVVEGPFSKDVILKIT
                     YN"
     gene            complement(359..559)
                     /gene="glgS"
                     /locus_tag="OO349_004144"
     CDS             complement(359..559)
                     /gene="glgS"
                     /locus_tag="OO349_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HCR5868996.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
BASE COUNT          249 a          182 c          153 g          239 t
ORIGIN      
        1 gtttagtgta gtcatgacaa aagatcctgg tgcggcctgt actgagcaat tcaatatttt
       61 agggagcttt ttcactacgg atattttgag tgattatagc catctggata tgggtaatgg
      121 actgctattg aagatatttc ataaagatgg aacagcaacg gaatttaacc gcttctcaca
      181 atttgcttct ttttcatcgt ctagtgcacc ttcggtcacc gcaccattca gggcagaact
      241 tagtgcgaac ccggcagaga ctgttgttga gggaccgttt agtaaagacg taatcctcaa
      301 aatcacctat aactagcatc taatacaaac actaaaacgg gccatcaggc ccgttatatc
      361 agtgctctaa ctccagctct cttgcctgca tcatctgttg gcaataccag gcataacgtg
      421 cgcaaaacca ggttctatgt tcttcatcca tgttaccagt aacagccaga atatcgaccg
      481 ggcgaccttc tgcgtgcatt ctggcaaaac tacgcgccag gaaatcgaaa ttattcaaag
      541 aattaagact atgatccata agcacatcct ccggttatcg catacctttt aaggccatat
      601 gctttttctg atttcagtat tccccaatca gtgtagtgcc tttaaacata acgtgcgtaa
      661 atatattgtc cccgatcaca ctttttagtg aaaaataaaa aaccatatta aaatactttc
      721 tcttccagaa cttctctaac atgtgttatt attaacaaag acaaatgtaa gcagggaaac
      781 tattccaagg taaatattgc gtcactaaat ggacattgga gtg
//
LOCUS       DAJTQY010000263          817 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.273, whole genome shotgun sequence.
ACCESSION   DAJTQY010000263 DAJTQY010000000
VERSION     DAJTQY010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..817
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.273"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            <1..448
                     /locus_tag="OO349_004145"
     CDS             <1..448
                     /locus_tag="OO349_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR5868997.1"
                     /translation="GKNWYEELKNFASDTIKIAYTGDGRVVWVGKDVTGIEPRNASVI
                     EVPDITTNRRITAPGYWFYRNDEFVFDYRLKAEDERDALLAQVSARTGEWEEDLLLGL
                     ISDEDKEKLKACRIYAKSLQAMDFSTITDKSSYNAIEWPASPEGSS"
     gene            472..>817
                     /locus_tag="OO349_004146"
     CDS             472..>817
                     /locus_tag="OO349_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCR5868998.1"
                     /translation="MSVVISGALTDGAGIPMSGYHIILKSRVNTPEVVMNTVADVMTG
                     NDGEYCFHARTGKYGVYLKQDWRNEYNVGDIAVYEDSKPGTLNDFLIAPDEGDLKPDV
                     VKRFEEMVAQAQQ"
BASE COUNT          225 a          179 c          228 g          185 t
ORIGIN      
        1 cggaaaaaac tggtatgaag agctgaagaa tttcgccagc gacacaataa aaatagctta
       61 caccggagat ggtcgcgtgg tgtgggtcgg taaggatgtg acaggcatcg agccacgcaa
      121 tgccagcgtt atcgaagttc cggatattac cactaaccga cggattaccg cgccgggtta
      181 ctggttttac cgcaatgatg aatttgtctt tgactacaga ctcaaagcgg aagatgagcg
      241 tgatgccctt ctggctcagg tcagtgcccg gacaggggaa tgggaagaag acctgctgct
      301 ggggttaatc agcgacgaag ataaagaaaa gctgaaagcc tgtcgtattt acgcgaaatc
      361 gctgcaggcg atggatttca gcaccatcac tgataaatcc tcatacaacg ccattgaatg
      421 gcccgcctct ccggaaggtt cttcctgatt taatttatcg cgagaaaaac aatgtctgta
      481 gtgatatcag gtgcgctgac tgatggcgca ggtatcccca tgtccggata ccatattatt
      541 ctgaaatccc gggtaaacac cccggaagtg gtgatgaaca ctgttgctga tgtgatgaca
      601 ggaaacgatg gtgaatactg tttccatgcg cggactggaa aatatggtgt gtatctgaaa
      661 caggactggc gcaacgagta caacgttggc gacattgctg tatatgagga ctcaaagccc
      721 ggcacgctga atgactttct gattgctcct gatgagggcg acctgaaacc ggatgtcgtc
      781 aaacgctttg aggaaatggt ggcgcaggcg cagcaga
//
LOCUS       DAJTQY010000264          812 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.274, whole genome shotgun sequence.
ACCESSION   DAJTQY010000264 DAJTQY010000000
VERSION     DAJTQY010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..812
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.274"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(<1..781)
                     /gene="uacT"
                     /locus_tag="OO349_004147"
     CDS             complement(<1..781)
                     /gene="uacT"
                     /locus_tag="OO349_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HCR5868999.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKDAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGLMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTM"
BASE COUNT          258 a          200 c          191 g          163 t
ORIGIN      
        1 tcatggtcag aatggaaacg ggatcgaaaa tcggcatacc aaacgacatc ggggtgacaa
       61 tcgcaaacca cgaggcatca tgtagcccgg ataaattgac ttcattcatc atccacgaaa
      121 gtaaaaagcc aaatacaatc cccagtaata cggcgacgtt ggacattaat cctttcgcat
      181 agcgagtaat gagcaagata aaaattaaga cggcaaagga gatacctaaa taaacgggat
      241 taccatattg cggattccct ttacctccag cggcccagtc aatacccacc tgaatgatgc
      301 taagcccaat agaagtaata accacaccgg taaccagtgg cgggaataaa ggcatcaagc
      361 gaccaataag tggcgctaat aatgtggtga taaaacccgc ggcgatagtg gcaccaaata
      421 tccccagcag gccgatatcc gggttcatcc caatggctat cattggtgtt acagcagcaa
      481 aggtcaccga cataatcacc ggcaggcgga tccccataaa gcggccgata ccgatacatt
      541 gcaataatgt gacgatcccg cagcaaaaga gatccgagct aatgagcatc gcaatagcat
      601 cttttgagag gcccagtcgg tcaccaatca taagaggaac agcgactgca cctgcgtaca
      661 tgacaaggac gtgttgcaga ccgagtatga tcagctttcc tggtgataat atgcggtcaa
      721 cctcatcagt tgggcggtct tgcccagaag atgagggaag ttgggaatct atggcgctca
      781 tcagtgtttc tcctttcgcc cgccagaata aa
//
LOCUS       DAJTQY010000265          811 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.275, whole genome shotgun sequence.
ACCESSION   DAJTQY010000265 DAJTQY010000000
VERSION     DAJTQY010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.275"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            <1..>811
                     /locus_tag="OO349_004148"
     CDS             <1..>811
                     /locus_tag="OO349_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HCR5869000.1"
                     /translation="KKLDVSCNKLTILTDLPENIKELIARDNFLTHISALPHYLITLD
                     VSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLKKLEVRNNELQTLPSLPSNLKI
                     LKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQENIIMRRFFYFENNQITTIPTNL
                     FRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQFRISLSDQNRLFLRQMLPQNL
                     HSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVSTQWTSFSTEENSRA"
BASE COUNT          259 a          187 c          122 g          243 t
ORIGIN      
        1 ttaaaaagct tgatgtgagc tgtaataaat taaccatcct tactgattta cctgaaaata
       61 ttaaagaact tattgcaaga gataatttct taacacatat atctgcatta ccacattatc
      121 taataacttt ggatgtgtcc gaaaatcaat tagagaatct gccgttatta ccagacacca
      181 tcaaatcact aagcgcagag tataataggt tatccacact gccttcatta cccttgaatt
      241 taaaaaaact tgaggttagg aacaacgaac tgcaaactct tccatctctg ccttctaatc
      301 ttaagatact taaggttgcg cacaaccatc ttactgaact gcccccttta cctaggagac
      361 tgcaacttct ttttgcatat agcaatagat taagcaactt accaaacatc caagaaaata
      421 ttatcatgag aagatttttt tattttgaaa acaaccaaat aactacaatc ccgacaaatc
      481 tttttcgttt agatcctcat ataactattg agattgcaaa taacccctta tcagatcaaa
      541 ctctgctatt cttaatacag caaacttcgg ttccaaattt taacgggcct cagtttcgta
      601 tttccctgtc agaccaaaac agactgtttt tacgccagat gttgccgcaa aatttacatt
      661 cgcgccatat cagagtcatc actgaagggg ggcagaactt tcagatcccc cctcttcccg
      721 aaactgtggc agcctggttt cctgaagcag atcgtcggga ggtttctaca caatggactt
      781 ctttttccac cgaggagaat tcccgggcat t
//
LOCUS       DAJTQY010000266          786 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.276, whole genome shotgun sequence.
ACCESSION   DAJTQY010000266 DAJTQY010000000
VERSION     DAJTQY010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..786
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.276"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            <1..>786
                     /locus_tag="OO349_004149"
     CDS             <1..>786
                     /locus_tag="OO349_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR5869001.1"
                     /translation="LEEKAHKKSLDDDKSRIGFWLQHFAGMQLRDITESKIYSAMQKM
                     TNRRHEENWKLRAEACRKKGKPVPEYTPKPASVATKATHLSFIKALLRAAEREWKMLD
                     KAPIIKVPQPKNKRIRWLEPHEAQRLIDECPEPLKSVVEFALATGLRRSNIINLEWQQ
                     IDMQRRVAWINPEESKSNRAIGVALNDTACRVLKKQIGNHHRWVFVYKESCTKPDGTK
                     APTVRKMRYDANTAWKAALRRAGIDDFRFHDLRHTWASWLVQA"
BASE COUNT          266 a          169 c          201 g          150 t
ORIGIN      
        1 ggctcgaaga gaaagcacat aagaaatcac tggacgatga caaaagccgg atcggattct
       61 ggcttcaaca tttcgcaggg atgcaactaa gagacatcac tgaatcaaaa atttattcag
      121 caatgcagaa aatgacgaac cggcgtcatg aggaaaactg gaaactcagg gcagaagcat
      181 gcagaaaaaa agggaaacct gttccagaat acacgccaaa accagcgtcc gttgcaacga
      241 aggctacgca tctttcattt ataaaggccc tactaagagc cgcagagcgt gaatggaaaa
      301 tgctggataa ggcaccaatt attaaagtgc ctcaaccaaa gaataaacgg atccgctggc
      361 tggagcccca tgaagcacaa aggctgattg atgaatgtcc ggagccatta aagtctgttg
      421 ttgaatttgc actggcaaca ggcttaagac gctcgaacat catcaacctt gaatggcaac
      481 aaatagatat gcagcgccgg gtggcatgga taaacccgga agagagtaaa tcaaaccgcg
      541 caattggcgt tgcgctgaat gatactgcat gtcgcgtatt gaaaaaacaa atcgggaatc
      601 atcaccgttg ggtatttgtg tacaaggaaa gctgtaccaa accagacgga acgaaagcgc
      661 caacagtaag gaagatgcgg tatgacgcaa acacagcctg gaaagcggcg ctgagacggg
      721 ctggtattga tgatttcaga tttcacgact tgagacacac ctgggcaagt tggctggttc
      781 aagccg
//
LOCUS       DAJTQY010000267          747 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.277, whole genome shotgun sequence.
ACCESSION   DAJTQY010000267 DAJTQY010000000
VERSION     DAJTQY010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..747
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.277"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            <1..>747
                     /locus_tag="OO349_004150"
     CDS             <1..>747
                     /locus_tag="OO349_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
                     /protein_id="HCR5869002.1"
                     /translation="DVLLRIHHVIGELPTYGYRRVWALLRRQAELDGMPAINAKRVYR
                     LMRQNALLLERKPAVPPSKRAHTGRVAVKESNQRWCSDGFEFCCDNGERLRVTFALDC
                     CDREALHWAVTTGGFNSETVQDVMLGAVERRFGNDLPSSPVEWLTDNGSCYRANETRQ
                     FARMLGLEPKNTAVRSPESNGIAESFVKTIKRDYISIMPKPDGLTAAKNLAEAFEHYN
                     EWHPHSALGYRSPREYLRQRACNGLSDNRCL"
BASE COUNT          180 a          177 c          232 g          158 t
ORIGIN      
        1 ggatgtgctt ctccgtatac accatgttat cggagagctg cccacgtatg gttatcgtcg
       61 ggtatgggcg ctgcttcgca gacaggcaga acttgatggt atgcctgcga tcaatgccaa
      121 acgtgtttac cggctcatgc gccagaatgc gctgttgctt gagcgaaaac ctgctgtacc
      181 gccatcgaaa cgggcacata caggcagagt ggccgtgaaa gaaagcaatc agcgatggtg
      241 ctctgacggg ttcgagttct gctgtgataa cggagagaga ctgcgtgtca cgttcgcgct
      301 ggactgctgt gatcgtgagg cactgcactg ggcggtgact accggcggct tcaacagtga
      361 aacagtacag gacgtcatgc tgggagcggt ggaacgccgc ttcggcaacg atcttccgtc
      421 gtctccagtg gagtggctga cggataatgg ttcatgctac cgggctaatg aaacacgcca
      481 gttcgcccgg atgttgggac ttgaaccgaa gaacacggcg gtgcggagtc cggagagtaa
      541 cggaatagca gagagcttcg tgaaaacgat aaagcgtgac tacatcagta tcatgcccaa
      601 accagacggg ttaacggcag caaagaacct tgcagaggcg ttcgagcatt ataacgaatg
      661 gcatccgcat agtgcgctgg gttatcgctc gccacgggaa tatctgcggc agcgggcttg
      721 taatgggtta agtgataaca gatgtct
//
LOCUS       DAJTQY010000268          734 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.278, whole genome shotgun sequence.
ACCESSION   DAJTQY010000268 DAJTQY010000000
VERSION     DAJTQY010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..734
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.278"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(<1..>734)
                     /locus_tag="OO349_004151"
     rRNA            complement(<1..>734)
                     /locus_tag="OO349_004151"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          140 a          246 c          161 g          187 t
ORIGIN      
        1 aatcctgttt gctccccacg ctttcgcacc tgagcgtcag tcttcgtcca gggggccgcc
       61 ttcgccaccg gtattcctcc agatctctac gcatttcacc gctacacctg gaattctacc
      121 cccctctacg agactcaagc ttgccagtat cagatgcagt tcccaggttg agcccgggga
      181 tttcacatct gacttaacaa accgcctgcg tgcgctttac gcccagtaat tccgattaac
      241 gcttgcaccc tccgtattac cgcggctgct ggcacggagt tagccggtgc ttcttctgcg
      301 ggtaacgtca atgagcaaag gtattaactt tactcccttc ctccccgctg aaagtacttt
      361 acaacccgaa ggccttcttc atacacgcgg catggctgca tcaggcttgc gcccattgtg
      421 caatattccc cactgctgcc tcccgtagga gtctggaccg tgtctcagtt ccagtgtggc
      481 tggtcatcct ctcagaccag ctagggatcg tcgcctaggt gagccgttac cccacctact
      541 agctaatccc atctgggcac atccgatggc aagaggcccg aaggtccccc tctttggtct
      601 tgcgacgtta tgcggtatta gctaccgttt ccagtagtta tccccctcca tcaggcagtt
      661 tcccagacat tactcacccg tccgccactc gtcagcgaaa cagcaagctg cttcctgtta
      721 ccgttcgact tgca
//
LOCUS       DAJTQY010000269          733 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.279, whole genome shotgun sequence.
ACCESSION   DAJTQY010000269 DAJTQY010000000
VERSION     DAJTQY010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..733
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.279"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            <1..>733
                     /locus_tag="OO349_004152"
     CDS             <1..>733
                     /locus_tag="OO349_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5869003.1"
                     /translation="SITTNRYASGYATLTEAVSAQDERNRKRDKNSHDGSTISLSQPL
                     GNIGNLNFNTTRYNSSRGTGNTRSTSLSYSTVWRGITFSINWAKNDLLTSHKWKVDRK
                     LSVGISVPLSLGDENQIYASSQMSRSGEQGNNYQVSLSGQNSGGVWWDVATNITNAHQ
                     SQPKSTMNIVQVGKNGSYGQFSSHYSSSENMKQLGANLSGGILITRDGLTFGQNVDGT
                     LALIEAPGATGVNVNGWPGLSTDFRG"
BASE COUNT          228 a          139 c          188 g          178 t
ORIGIN      
        1 tctataacca cgaaccgtta tgcatcaggt tatgccacac tgactgaagc agtaagtgca
       61 caggatgaac ggaacagaaa aagagataag aatagtcacg atggctctac gatatcctta
      121 tcccagcccc ttggtaatat cgggaatttg aacttcaata ccacgaggta taattcttcc
      181 cggggcacag gaaacacgcg gtctaccagc ctgagttata gtacggtatg gagaggcata
      241 acattctcca ttaactgggc aaaaaacgac ttgctgacat cgcataaatg gaaagtagat
      301 cggaaattaa gcgtgggtat ttcagtgcca ttatcgctgg gtgacgaaaa tcaaatttat
      361 gcgtcttcac agatgagtcg ttcgggagag cagggtaata actatcaggt ttcattatca
      421 ggtcagaata gtggtggtgt atggtgggat gtagcgacaa atataactaa cgctcatcag
      481 tcacaaccga agagcacgat gaacattgtg caagttggca aaaatggatc atatggtcag
      541 tttagtagcc attacagctc cagcgaaaac atgaaacagc taggtgcaaa tttaagcggt
      601 gggatactta ttacaaggga tggacttaca tttggtcaaa atgtggatgg gacattagcc
      661 cttatagagg cacccggagc aacgggggtt aacgttaatg gctggccggg actgagtact
      721 gattttcgtg ggt
//
LOCUS       DAJTQY010000270          727 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.280, whole genome shotgun sequence.
ACCESSION   DAJTQY010000270 DAJTQY010000000
VERSION     DAJTQY010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..727
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.280"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
BASE COUNT          205 a          137 c          160 g          225 t
ORIGIN      
        1 tggataatgc gattgcctgt agttcttcgt tagtcattgt ctgcacctgg aaagcaattt
       61 accggagtac ctcacactac ggcaaaaatg ttttattgtt gcaacttgcg taattattca
      121 ggtcagtttg gaacgcactg tataaacata aataacctga tactgttgtg tgttttcaaa
      181 gaaaacacag gtatttttca cataaacctt ccccttttca tattcgacca cttaatcaaa
      241 aaacggtttt atacttgttg cccccactgg ggttaaatgt gagacatgtc gcactttgtg
      301 ccatgtgtcc tcgctgattt taggcgcggt tcattcattc tttctgttgg cataaagtat
      361 ggtatcggtt gggcgaatga aagggactcg ggtcatggtc agacaatacc tgaggcgtta
      421 attcgttgaa agagattgtg aatgggggaa taactgagtc ttaactgctg tgcgatagcc
      481 cggacagata gttgctcgtc gaatctgaga cgtaatgatt ctggcaaata agtgcgaaag
      541 ccggtgatat ctagtgttgt ttgagagttc atcgttcgcg tccattaata cagatggacg
      601 cgattgttgc cagacaggat aattttcaca agacatcgca gatagagtac ttacgataat
      661 tttttatttt atacaacaga tgcccacgaa aagaattatt ttcaatagac acctgcatta
      721 cacaaag
//
LOCUS       DAJTQY010000271          721 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.281, whole genome shotgun sequence.
ACCESSION   DAJTQY010000271 DAJTQY010000000
VERSION     DAJTQY010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..721
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.281"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            87..>721
                     /locus_tag="OO349_004153"
     CDS             87..>721
                     /locus_tag="OO349_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR5869004.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRCE
                     EINRLNRQLRVEKILRSGIQPLHRKCSFANYRAQNDGQRHALSQAKSIADELMTGCTN
                     FVFSGKPGTGKNHLAAAIGNRLMAKGRSVIIVTVSDVMSVLHESYDNGKSGEKFLQEL
                     CGVDLLVLDEIGMQRDTKNEQVVLNQIVDRRTASLRGVGMLTNINHAAMNTL"
BASE COUNT          179 a          175 c          217 g          150 t
ORIGIN      
        1 ttcggcctga gacgttgctc cgacaggtta acaccgtctc gttgccggac agtgcgatcc
       61 cgctaggctt cagggggtaa cagaccatga aaaatattgc gacaggaggc gttctggagc
      121 gtatccgcag actgacccca ccacatgtaa ccgccccatt cagaacggtt gcggagtggc
      181 gcgagtggca acttgctgaa ggccagaaac gttgcgagga gatcaaccgc ctgaatcgtc
      241 agttgcgggt ggaaaaaatc ctccgctccg gcatccagcc gttgcaccgg aagtgttcgt
      301 ttgcgaatta ccgggcgcag aacgacggtc agcgacatgc actgagtcag gcgaaatcca
      361 tagctgacga actgatgaca ggctgtacga attttgtgtt cagcggtaag cccggcaccg
      421 gaaaaaatca tcttgcagcc gcgattggca accggctgat ggcgaagggg cgtagcgtga
      481 ttatcgtcac cgtgtccgat gtcatgagcg tgttgcatga gagctacgac aacggcaaat
      541 ccggtgaaaa atttttacag gagctttgtg gtgttgacct gctggtcctg gatgaaattg
      601 gcatgcagcg ggatacgaaa aacgagcagg tggtactgaa ccagattgtt gatcgccgga
      661 cggcatcgtt acgcggtgtg gggatgctga caaatattaa ccatgcagcg atgaatacac
      721 t
//
LOCUS       DAJTQY010000272          697 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.282, whole genome shotgun sequence.
ACCESSION   DAJTQY010000272 DAJTQY010000000
VERSION     DAJTQY010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..697
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.282"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            115..>697
                     /locus_tag="OO349_004154"
     CDS             115..>697
                     /locus_tag="OO349_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5869005.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSIL"
BASE COUNT          158 a          140 c          170 g          229 t
ORIGIN      
        1 tttaggatta atcctttttt cgtgagtaat cttatcgcca gtttggtctg gtcaggaaat
       61 agttatacat catgacccgg actccaaatt caaaaatgaa attaggagaa gagcatgagt
      121 tctgccaaga agatcgggct atttgcctgt accggtgttg ttgccggtaa tatgatgggg
      181 agcggtattg cattattacc tgcgaaccta gcaagtatcg gtggtattgc tatctggggt
      241 tggattatct ctattattgg tgcaatgtcg ctggcgtatg tatatgcccg actggcaaca
      301 aaaaacccgc aacaaggtgg cccaattgct tatgccggag aaatttcccc tgcatttggt
      361 tttcagacag gtgttcttta ttaccatgct aactggattg gtaacctggc gattggtatt
      421 accgctgtat cttatctttc caccttcttc ccagtattaa atgatcctgt tccggcgggt
      481 atcgcctgta ttgctatcgt ctgggtattt acctttgtaa atatgctcgg cggtacctgg
      541 gtaagccgtt taaccactat tggtctggtg ctggttctta ttcctgtggt gatgactgct
      601 attgttggct ggcattggtt tgatgcggca acttatgcag ctaactggaa tactgcggat
      661 accactgatg gtcatgcgat cattaaaagt attctgc
//
LOCUS       DAJTQY010000273          672 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.283, whole genome shotgun sequence.
ACCESSION   DAJTQY010000273 DAJTQY010000000
VERSION     DAJTQY010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..672
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.283"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
BASE COUNT          211 a          165 c          130 g          166 t
ORIGIN      
        1 ccccccgaca gacctgtgct atcggaaaga aaaaaggcca tcagcaaaac tctgatgacc
       61 tgaatcacct ttaccaatat tgtatgaaaa aacacgcacg actaattgac aataattttc
      121 atttccatta aaaaagatcg cgtagcattc ttaattcatg aggaacttta cccgccagca
      181 atctgagtag cgaaagctgt tcatccccaa tgtttttgct ggcgggtcct tttttctacg
      241 gtcctcctcc ccggaggggg gcatttcatt atttttctaa tcgttactgg tgagaaggcg
      301 gaagaaaaag ccagttcttc gcccctaaat aacagcatga ttccagtcca tgcaaaatct
      361 gatttccagc tcacacatac cgagcgctgt acaaaaaacc gccagccaag gcagttaaga
      421 gtgtgttccc ggggtttgct tagaatattt tgtaagttgt ccgaagagat atttacaaca
      481 ccagaatgat gaatcatcag tcccctgcca gaggttcact acgtaacctg accgacaatg
      541 caccattctg gtgctgaaaa aagagcactg gaactgcaac aaaacaacat cacagaacag
      601 gagatggtaa ggagtcagac attcccacac aatattgtgt cagtgcaaat aacaaccttc
      661 gtctcaaatc tc
//
LOCUS       DAJTQY010000274          647 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.284, whole genome shotgun sequence.
ACCESSION   DAJTQY010000274 DAJTQY010000000
VERSION     DAJTQY010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..647
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.284"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            60..>647
                     /locus_tag="OO349_004155"
     CDS             60..>647
                     /locus_tag="OO349_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
                     /protein_id="HCR5869006.1"
                     /translation="MLPRFADIFQQGNRWLNWLEKQPEGAVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCCHTKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPYCPWQHIVF
                     TLPCQYWSLVFHNRWLLAEMSRIAADVILEICHQADVEPGIFTVIHTWGRDQQWHPHI
                     HLSTTAGGVTSGHTWKNLHFYARKVMSMWRYRITRL"
BASE COUNT          137 a          173 c          191 g          146 t
ORIGIN      
        1 atgtactgcc cggagtgtgg cacggttcac tggccggacg gagtcgcccc gcccttctga
       61 tgctcccccg ttttgccgat atttttcagc agggaaaccg ctggcttaac tggctggaga
      121 aacaaccgga aggggcagtc cgtccggtgg taattgaatc agtgacaaaa atcatggcct
      181 gcgggaccac actgatgggc tacacgcagt ggtgttgttc atcaccggac tgttgccata
      241 caaaaaaggt ctgcttccgg tgtaaaagcc gctcctgccc gcactgcggg gtgaaggctg
      301 gcgcacagtg gatacagtat ctgctgagcc tggtccccta ctgcccgtgg cagcatattg
      361 tgttcacact tccctgccag tactggtccc tggtgttcca caaccggtgg ttactggcag
      421 agatgagccg cattgcagcg gatgtgatac tggaaatctg ccatcaggca gatgtggagc
      481 cggggatatt cacggtgatc cacacatggg ggcgtgacca gcagtggcat ccgcatatcc
      541 atttatcgac aactgccggt ggtgtgacgt cgggccacac ctggaaaaat cttcattttt
      601 acgcccgtaa ggtgatgagc atgtggcgct accggataac acggttg
//
LOCUS       DAJTQY010000275          637 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.285, whole genome shotgun sequence.
ACCESSION   DAJTQY010000275 DAJTQY010000000
VERSION     DAJTQY010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..637
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.285"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            10..637
                     /locus_tag="OO349_004156"
                     /pseudo
     CDS             10..637
                     /locus_tag="OO349_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          172 a          151 c          164 g          150 t
ORIGIN      
        1 atttgacctg tgaaacgatt acaggcattt aaattccagt taagaccagg tggtcaacag
       61 gagtgtgaaa tgaggcgctt cgcaggcgca tgtcgtttcg ttttcaatcg tgctctggca
      121 cgtcagaatg agaatcatga ggccggtaat aaatacatcc cttacgggaa aatggcttcc
      181 tggctggttg agtggaaaaa tgccactgaa acgcaatggc ttaaagattc tccctcacag
      241 ccattgcaac agtcactgaa agaccttgag cgggcttaca aaaacttctt ccggaagcgg
      301 gctgcttttc cccgattcaa aaagcggtga cagaatgatg cattccgcta cccgcagggc
      361 gttaagctcg atcaggaaaa cagccgtatt tttctgccga aactgggctg gatgcgctac
      421 cggaatagcc gtcaggtcac gggtgttgtg aaaaatgtca ctgtcagcca gtcctgcggt
      481 acgtggtaca tcagtattca gacagaaagt gaagtatcca ctccggctca cccttcagca
      541 tcaatggtcg ggctggatgc tggcgtggct aaactcgcca cgctgtcaga tggcacagtc
      601 tttgagcctg taaacagttt tcagaaaaac cagaaga
//
LOCUS       DAJTQY010000276          604 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.286, whole genome shotgun sequence.
ACCESSION   DAJTQY010000276 DAJTQY010000000
VERSION     DAJTQY010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..604
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.286"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            88..153
                     /locus_tag="OO349_004157"
                     /pseudo
     CDS             88..153
                     /locus_tag="OO349_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
     gene            381..>604
                     /locus_tag="OO349_004158"
     CDS             381..>604
                     /locus_tag="OO349_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HCR5869007.1"
                     /translation="MRVEICIAKEKITKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWES"
BASE COUNT          193 a          125 c          140 g          146 t
ORIGIN      
        1 atagatgcac aaaaaacggg caaaactacc tggttcgcaa aactgcgtct aaagttaaac
       61 tgggacctcg cgagcaaggg tgagacgatg gcgctttaca caattggtga agtggcgttg
      121 ctttgtgata ttaatcctgt cacgttacgc gcgtgacgca acatttttaa aaatcgacga
      181 agatcgtcca ggagcgccgt ttttcaaggg ttggatagat gattcgttta ccatgtcgac
      241 gaacttcgac gaatttcgcc gataaaaagt gtcccataca tgccccattg atgccccata
      301 aacaatatca ggaattgaca ctggttatcc atacagataa aaataacacc tgtacataac
      361 tacagttaca ggagggtgtt atgcgtgttg agatctgtat cgctaaagaa aaaatcacta
      421 aaatgccaaa cggtgctgtg gatgcgttaa aggaagaatt aacccgacgc atcagtaaac
      481 gttatgacga tgtagaggtg atcgtaaaag ccaccagcaa cgatggcctt tctgttacac
      541 gcaccgcaga taaggattct gcaaaaacat ttgttcagga gactctgaaa gatacctggg
      601 agtc
//
LOCUS       DAJTQY010000277          599 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.287, whole genome shotgun sequence.
ACCESSION   DAJTQY010000277 DAJTQY010000000
VERSION     DAJTQY010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..599
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.287"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            <1..>599
                     /locus_tag="OO349_004159"
     CDS             <1..>599
                     /locus_tag="OO349_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR5869008.1"
                     /translation="TDNGYTSGNPFNGLTPLKKSKIEPDPLTRDEFIRFIEACRHQQT
                     KNLWIIAVYTGIRHGELVSLAWEDIDLKARTITIRRNYTKLGEFTPPKTDAGTGRTIH
                     LVQPAIDALKSQAEMTMLGKQHSVEVKQREYGRSTVHKCTFVFSPQVIKQRQFSGPHY
                     KVDSIRESWTSILKRAGLRHRKSYQSRHTYACWSLAAGA"
BASE COUNT          194 a          131 c          132 g          142 t
ORIGIN      
        1 acagataatg gttatacatc aggaaatcca tttaacggtc tgacaccatt aaaaaagtcc
       61 aagatagaac cagatcccct cacccgtgac gaatttattc gttttattga ggcttgccgt
      121 catcaacaaa caaaaaacct gtggattatc gctgtataca cgggtattcg tcacggggag
      181 ctggtatcac tggcctggga agatatagac cttaaagcga ggactataac catccgccgg
      241 aattatacaa aactaggcga attcactcca ccaaaaactg atgctggtac cggaagaacg
      301 attcatctag ttcaaccagc tattgatgct cttaaaagcc aagcggaaat gaccatgctt
      361 ggaaaacagc attctgtaga ggtaaagcag agggaatatg ggagaagtac tgtgcataaa
      421 tgcacttttg tttttagtcc tcaggtaata aaacagcggc agttttccgg accgcactat
      481 aaggttgact ccatcaggga gtcatggaca agtatcttaa aacgcgcagg tctgagacac
      541 agaaaatcgt atcaatccag gcatacctat gcatgctggt cacttgccgc aggagctaa
//
LOCUS       DAJTQY010000278          591 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.288, whole genome shotgun sequence.
ACCESSION   DAJTQY010000278 DAJTQY010000000
VERSION     DAJTQY010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..591
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.288"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            1..591
                     /locus_tag="OO349_004160"
                     /pseudo
     CDS             1..591
                     /locus_tag="OO349_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          147 a          150 c          164 g          130 t
ORIGIN      
        1 gaaagagctg aaacgggtta ccgacgaacg ggacatatta aaaaaagccg cggcgtactt
       61 cgcaaagctg tccgactgag gtacgccttt atccgtgaca actcctgttg ctggcctgtt
      121 cgcctgctct gtcgggtgct ggatgttcat cccagtggtt tttacgcctg gcttcagcag
      181 ccgcattcac aacgccatca ggcagacctg agactgacag gacagattaa acagttctgg
      241 ctggaatcgg gatgcgtcta tggttatcgc aaaatccatc tggatctgcg tgacagcggg
      301 caacagtgcg gagtaaacag agtctggaga ctgatgaaac gtgtcggaat aaaggctcag
      361 gtcggatacc gaagcccgcg ggcacgtaaa ggcgaggcca gtatcgtgtc acccaacagg
      421 ctccagcgac agttcaatcc ggatgctcct gatgagcgtt gggtaacgga cataacctac
      481 atcaggaccc acgaaggctg gctgtatctt gccgttgttg ttgatctgtt ctcacgcaaa
      541 attatcggct ggtccatgca atcccggatg acaaaggaca ttgtcctgaa c
//
LOCUS       DAJTQY010000279          583 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.289, whole genome shotgun sequence.
ACCESSION   DAJTQY010000279 DAJTQY010000000
VERSION     DAJTQY010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..583
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.289"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(50..190)
                     /locus_tag="OO349_004161"
     CDS             complement(50..190)
                     /locus_tag="OO349_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5869009.1"
                     /translation="MSEENKENGFNHVKTFTKIIFIFSVLVFNDNESKITDAAVNLFI
                     QI"
     gene            329..>583
                     /locus_tag="OO349_004162"
     CDS             329..>583
                     /locus_tag="OO349_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HCR5869010.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYT"
BASE COUNT          175 a          106 c          123 g          179 t
ORIGIN      
        1 acatacgaaa gtgaacatta gtatgaaata gcgcattata aatttctcat taaatctgga
       61 taaataaatt gacggcggca tcggtaattt tagattcgtt atcattaaaa actaatacag
      121 aaaaaataaa tataattttg gtgaatgttt tgacatgatt aaatccattt tctttatttt
      181 cttctgacat aatacgttac tccctttaat tattaacgat atgtttcgta tttttcttta
      241 aatctgcatg taacagaatg ttaataagac atgattgttg cggcaggatc tgcaataatt
      301 aacaattatc cacttcacag agaatgctat gcagtacccg attaacgaga tgttccagac
      361 cctgcaaggt gagggttact ttaccggcgt ccccgccatt tttattcgtt tacagggatg
      421 cccggttggc tgtgcctggt gcgacaccaa acacacctgg gaaaagcttg aggatcggga
      481 agtctccctt ttcagcattc tggcgaagac caaagagagt gataagtggg gggcggcgag
      541 cagtgaagac ttgctggctg tcattggtcg tcagggatat acc
//
LOCUS       DAJTQY010000280          581 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.290, whole genome shotgun sequence.
ACCESSION   DAJTQY010000280 DAJTQY010000000
VERSION     DAJTQY010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..581
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.290"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(<1..181)
                     /locus_tag="OO349_004163"
     CDS             complement(<1..181)
                     /locus_tag="OO349_004163"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter"
                     /protein_id="HCR5869011.1"
                     /translation="MMATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPE
                     GVVTAFWTLSASGELW"
     gene            complement(190..>581)
                     /locus_tag="OO349_004164"
     CDS             complement(190..>581)
                     /locus_tag="OO349_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCR5869012.1"
                     /translation="ETIAKAQAAFARTDSVGQQRMAALHNGKRDNLEISPNLWAGVGL
                     VRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPHLEEAYRVGELLFPHLDVAIP
                     EIPQPQPLNPQGEAVANDFIPRKVAQS"
BASE COUNT          119 a          187 c          161 g          114 t
ORIGIN      
        1 gccacagttc gccgctggcg gagagcgtcc agaacgccgt caccactcct tccggtgaag
       61 gcaaaatacg cgtcgacagc cagccaaccg aggaggccag ttgccacacc gccacgatgc
      121 ccaccggtaa aaaccagggg gcaacgcgca ataaccactt cttcactggc gttgccatca
      181 tcgtgttcct tagctttgcg cgactttacg ggggataaaa tcatttgcca ccgcttcgcc
      241 ttgcggattc agcggctgcg gctggggaat ttccggaatg gcgacatcca gatgtgggaa
      301 cagcaactcg ccaacccgat acgcttcttc cagatgcggg tagcccgaaa gcacaaagct
      361 gtcgatgcca agcgcggcgt attcgttgat tcgcgcggcg accgtaggac catcgcccac
      421 cagcgccgtt ccggcaccgc cgcgcactaa gccaacgccc gcccataaat tggggctgat
      481 ctccagattg tcgcgcttgc cgttatgtag cgccgccatt cgctgttgtc ctacggaatc
      541 cgtccgggcg aatgcggctt gtgctttggc gatagtctca t
//
LOCUS       DAJTQY010000281          571 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.291, whole genome shotgun sequence.
ACCESSION   DAJTQY010000281 DAJTQY010000000
VERSION     DAJTQY010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..571
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.291"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            153..>571
                     /locus_tag="OO349_004165"
     CDS             153..>571
                     /locus_tag="OO349_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2184 domain-containing protein"
                     /protein_id="HCR5869013.1"
                     /translation="MITFDQATVDSSGAFLIGELERLDQTLNLPLVGYTWSRDIQLRE
                     DASIADDISSWTNTSFAAAGTGANPNGKNWVGKDSTAIAGVNVDIGKSGNPLNLWGME
                     LGWTVIELQAAQQVGRPIDTQKYDGMQLKWQMDNDEQV"
BASE COUNT          149 a          136 c          166 g          120 t
ORIGIN      
        1 tccactgatg aatccatcaa agtgcctctg ggtggattca tgtccacgtc agtcagtggc
       61 aaaaacgtgg tgctgaccaa cgctgaattc acaggtgccg gtgatgctga cggcaatgca
      121 gaaatttcct ggaagattta aggaacagac gaatgattac ttttgatcag gcaaccgttg
      181 acagctctgg tgcctttctc atcggggagc tggagagact cgaccagacg ctgaacctgc
      241 cactggtggg gtacacctgg agccgcgata ttcaactgcg tgaagacgcc tccatcgcag
      301 atgacatttc cagctggacg aataccagct tcgccgctgc gggtactggc gcaaatccga
      361 atggaaaaaa ctgggtaggc aaagactcaa ccgctattgc tggcgtgaac gtggatatcg
      421 gcaaatccgg taacccgctg aacctgtggg ggatggaact tggctggacg gtcatagaat
      481 tgcaggctgc tcagcaggtc ggccgcccga ttgatacgca gaagtatgac ggtatgcaac
      541 tgaaatggca gatggataac gatgaacagg t
//
LOCUS       DAJTQY010000282          567 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.292, whole genome shotgun sequence.
ACCESSION   DAJTQY010000282 DAJTQY010000000
VERSION     DAJTQY010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..567
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.292"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            <1..>567
                     /locus_tag="OO349_004166"
     CDS             <1..>567
                     /locus_tag="OO349_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCR5869014.1"
                     /translation="TAFKVIETIRPKLACSRCDVIVQAPLPPKPIERGYASAGLLARI
                     LVSKYMEHIPLYRQSEIYARQGVELSRNTMVRWVSEMADKLRPLYIALNDYVLEAGKV
                     HADDTPVKVLAPGNGKTKTGRLWVYVRDDRNAGSSLPAAVWFAYSADRKGEHPQLHLA
                     KYQGVLQADAYAGYNVLYETGRVKEAGC"
BASE COUNT          143 a          141 c          163 g          120 t
ORIGIN      
        1 ataccgcctt taaagttatc gaaaccatac gtcccaaact ggcctgtagc cggtgtgatg
       61 tcatcgttca ggcaccactt cctcctaaac cgatcgaacg cggttatgcc agtgcagggt
      121 tacttgcacg gatcctggtc agcaaatata tggaacatat ccctttatat cgccagtcag
      181 aaatatacgc gcgacagggc gtggagctga gccgtaatac catggtgcgc tgggtatcag
      241 aaatggcaga caaactccgt cctctgtata tagcgctgaa tgactatgtt ctggaggcag
      301 gaaaggtgca cgcagatgac actccggtga aagtactggc cccggggaac ggaaagacga
      361 aaacgggtcg tctgtgggta tacgtcaggg atgatcgtaa tgcgggttca tccctgccgg
      421 cagccgtctg gttcgcgtat tcggcagatc gcaaaggaga acatccgcag ctccacctgg
      481 caaagtatca gggcgtactg caggctgatg cctatgcagg ttataacgta ctgtacgaaa
      541 cgggccgggt gaaggaagcc gggtgcc
//
LOCUS       DAJTQY010000283          552 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.293, whole genome shotgun sequence.
ACCESSION   DAJTQY010000283 DAJTQY010000000
VERSION     DAJTQY010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..552
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.293"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(<1..175)
                     /locus_tag="OO349_004167"
     CDS             complement(<1..175)
                     /locus_tag="OO349_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5869015.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDP"
     gene            complement(240..488)
                     /locus_tag="OO349_004168"
     CDS             complement(240..488)
                     /locus_tag="OO349_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HCR5869016.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
BASE COUNT          174 a          127 c          139 g          112 t
ORIGIN      
        1 gtggatcgga cttcagattg ataaagggga cgccgcgtag ctccgacgac agcataaaaa
       61 tcttgccgcc aattttaaaa acatcgaact ccgggccaaa aggccagcaa agctcgacaa
      121 agggtaactc aagagctagg cgtttcgccg tttcgtgcag tgattgctta tccataaacg
      181 ttcctttagg cgaaggagaa taagcaaagt atgccgcgaa gttcggcgat aatcgacgtt
      241 taatccgcca gcgagaacca gcgtcgccag ataaagcgca gaacaaaata ctcaatagcg
      301 cccagcacta aaaaccacag acaaaacaat aaagtgtaaa gctggctaag atccatcaga
      361 tggaacatgg tcaccagttt ttgtgccagc gtcagcccca gtgcgggggc gggcagcagc
      421 aggcaagaga ggaaagccat cagcaaaatg cctgctgcgg tggtcaaggt ttctaaaggg
      481 tgtttcataa cattgttaaa tgtaagttaa aacaccattg tcagggatat tcttctgtaa
      541 ggcaattccc gg
//
LOCUS       DAJTQY010000284          543 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.294, whole genome shotgun sequence.
ACCESSION   DAJTQY010000284 DAJTQY010000000
VERSION     DAJTQY010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..543
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.294"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(<1..>543)
                     /locus_tag="OO349_004169"
     rRNA            complement(<1..>543)
                     /locus_tag="OO349_004169"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          112 a          165 c          130 g          136 t
ORIGIN      
        1 ccctcccgaa ggttaagcta cctacttctt ttgcaaccca ctcccatggt gtgacgggcg
       61 gtgtgtacaa ggcccgggaa cgtattcacc gtggcattct gatccacgat tactagcgat
      121 tccgacttca tggagtcgag ttgcagactc caatccggac tacgacgcac tttatgaggt
      181 ccgcttgctc tcgcgaggtc gcttctcttt gtatgcgcca ttgtagcacg tgtgtagccc
      241 tggtcgtaag ggccatgatg acttgacgtc atccccacct tcctccagtt tatcactggc
      301 agtctccttt gagttcccgg ccggaccgct ggcaacaaag gataagggtt gcgctcgttg
      361 cgggacttaa cccaacattt cacaacacga gctgacgaca gccatgcagc acctgtctca
      421 cggttcccga aggcacattc tcatctctga aaacttccgt ggatgtcaag accaggtaag
      481 gttcttcgcg ttgcatcgaa ttaaaccaca tgctccaccg cttgtgcggg cccccgtcaa
      541 ttc
//
LOCUS       DAJTQY010000285          528 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.295, whole genome shotgun sequence.
ACCESSION   DAJTQY010000285 DAJTQY010000000
VERSION     DAJTQY010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.295"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(<1..131)
                     /gene="csgC"
                     /locus_tag="OO349_004170"
     CDS             complement(<1..131)
                     /gene="csgC"
                     /locus_tag="OO349_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="involved in autoagglutination of curliated cells;
                     not involved in production of curli fibers; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HCR5869017.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDMYTIIPEVTLTQSCLCRVQI"
     gene            complement(190..>528)
                     /gene="csgA"
                     /locus_tag="OO349_004171"
     CDS             complement(190..>528)
                     /gene="csgA"
                     /locus_tag="OO349_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin major subunit CsgA"
                     /protein_id="HCR5869018.1"
                     /translation="GPNSELNIYQYGGGNSALALQTDARNSDLTITQHGGGNGADVGQ
                     GSDDSSIDLTQRGFGNSATLDQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVTQVG
                     FGNNATAHQY"
BASE COUNT          141 a          122 c          126 g          139 t
ORIGIN      
        1 aatatttgta ctctgcacag acaagattga gtaagagtaa cttcaggaat aatggtgtac
       61 atatcccctt gctgggtcgt attaaaggtt atctggctgg aaagtgccgc aaggagtaat
      121 aacgcattca tattcttctc ccgaaaaaaa acagggcttg cgccctgttt ctgtaataca
      181 aatgatgtat taatactgat gagcggtcgc gttgttacca aagccaacct gagtcacgtt
      241 gacggaggag ttcgatgcag tctggtcaac tgcagcaccg ttgccaccac cgaactgttt
      301 aacagtcatt tcagaatttt tgccgttcca ctgatcaaga gtggcgctgt taccgaagcc
      361 acgttgggtc agatcgattg agctgtcatc tgagccctga ccaacatctg caccattacc
      421 accgccatgc tgggtaatag tcaagtcaga gttacgggca tcagtttgca gagcaagtgc
      481 agagttaccg ccaccgtact ggtaaatgtt cagctctgaa tttgggcc
//
LOCUS       DAJTQY010000286          525 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.296, whole genome shotgun sequence.
ACCESSION   DAJTQY010000286 DAJTQY010000000
VERSION     DAJTQY010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..525
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.296"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            <1..>525
                     /locus_tag="OO349_004172"
     CDS             <1..>525
                     /locus_tag="OO349_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HCR5869019.1"
                     /translation="LAESGTVTLRKRRLPLEMMVWCIVGMALERKEPLHQIVNRLDIM
                     LPGNRPFVAPSAVIQARQRLGSEAVRRVFTKTAQLWHNATPHPHWCGLTLLAIDGVFW
                     RTPDTPENDAAFPRQTHAGNPALYPQVKMVCQMELTSHLLTAAAFGTMKNSENELAEQ
                     LIEQTGDNTLTLMD"
BASE COUNT          118 a          163 c          141 g          103 t
ORIGIN      
        1 ccttgccgaa tcaggtactg taacgctacg caagcgccgt cttcccctcg aaatgatggt
       61 ctggtgtatt gttggcatgg cgcttgagcg taaagaacct cttcaccaga ttgtgaatcg
      121 cctggacatc atgctgccgg gcaatcgccc cttcgttgcc cccagtgccg ttattcaggc
      181 ccgccagcgc ctgggaagtg aggctgtccg ccgcgtgttc acgaaaacag cgcagctctg
      241 gcataacgcc acgccgcatc cgcactggtg cggcctgacc ctgctggcca tcgatggtgt
      301 gttctggcgc acaccggata caccagagaa cgatgcagcc ttcccccgcc agacacatgc
      361 cgggaacccg gcgctctacc cgcaggtcaa aatggtctgc cagatggaac tgaccagcca
      421 tctgctgacg gctgcagcct tcggcacgat gaagaacagc gaaaatgagc ttgctgagca
      481 acttatagaa caaaccggcg ataacactct gacgttaatg gataa
//
LOCUS       DAJTQY010000287          524 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.297, whole genome shotgun sequence.
ACCESSION   DAJTQY010000287 DAJTQY010000000
VERSION     DAJTQY010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..524
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.297"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            <1..414
                     /locus_tag="OO349_004173"
     CDS             <1..414
                     /locus_tag="OO349_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5869020.1"
                     /translation="EGSWRKERRTSYSREFKVRLAKQALQPGAVVARIAREHDINNNL
                     LFKWKSQYEDGLLSDDDIQECMPVPVALTDTPEPTRPVTNPFWRNKHDERPEGAPGNV
                     PRCELHLKSGVVKLFDPLTPELLRALIREMKGGIR"
     gene            411..>524
                     /locus_tag="OO349_004174"
     CDS             411..>524
                     /locus_tag="OO349_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR5869021.1"
                     /translation="MITLPTGTKIWIIAGITDMRCGFNGLASKVQNTLKDDP"
BASE COUNT          139 a          132 c          147 g          106 t
ORIGIN      
        1 gaaggctcat ggcgaaaaga acggcgaacc agctatagcc gtgaatttaa ggtccgtctg
       61 gctaagcagg cattacagcc tggggctgtt gttgcccgga tcgccagaga acacgatatc
      121 aataataacc tgctgtttaa atggaaaagc cagtacgagg acggcttact gagcgatgat
      181 gacatacagg aatgcatgcc tgtcccggtg gcactgactg atacgccgga gccgaccaga
      241 ccagttacaa atcccttctg gcgtaacaag catgatgagc gccctgaggg ggctcccgga
      301 aacgtcccac ggtgcgagct gcatcttaaa tcaggtgtgg taaaactgtt tgaccctctc
      361 actccggaac tgttacgggc gctaatccgc gaaatgaaag ggggtatccg atgataacgc
      421 tgccgaccgg taccaaaatc tggatcatcg ctggcatcac agatatgcgt tgtggcttca
      481 atggcctggc ttcgaaggtg cagaacacgc tgaaagatga cccg
//
LOCUS       DAJTQY010000288          521 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.298, whole genome shotgun sequence.
ACCESSION   DAJTQY010000288 DAJTQY010000000
VERSION     DAJTQY010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..521
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.298"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            <1..142
                     /locus_tag="OO349_004175"
     CDS             <1..142
                     /locus_tag="OO349_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR5869022.1"
                     /translation="ISDEDREKLKAYRIYAKSLQAMDFSVITDKTSYNAIEWPASPEI
                     SS"
     gene            166..>521
                     /locus_tag="OO349_004176"
     CDS             166..>521
                     /locus_tag="OO349_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCR5869023.1"
                     /translation="MSVVISGALTDGAGISMSGYHIILKSRVNTPEVVMHTVADVMTG
                     NDGEYCFHARTGKYGVYLKQDWRNEYNVGDIAVYEDSKPGTLNDFLIAPDEGDLKPDV
                     VKRFEEMVAQAQQSAGA"
BASE COUNT          141 a          117 c          142 g          121 t
ORIGIN      
        1 aatcagcgac gaagaccggg aaaaactgaa agcgtaccgc atttacgcga aatcgctgca
       61 ggcgatggat ttcagtgtca tcactgataa aacctcatac aacgccattg aatggcccgc
      121 ttctccggaa atttcttcct gatttaattt atcgcgagaa aaactatgtc tgtagtgata
      181 tcaggtgcgc tgactgatgg cgcaggtatc tccatgtccg gataccatat tattctgaaa
      241 tcccgggtaa acaccccgga agtggtgatg cacactgttg ctgatgtgat gacaggaaac
      301 gatggtgaat actgtttcca tgcgcggact ggaaaatatg gtgtgtatct gaaacaggac
      361 tggcgcaacg agtacaacgt tggcgacatt gctgtatatg aggactcaaa gcccggcacg
      421 ctgaatgact ttctgattgc tcctgatgag ggcgacctga aaccggatgt cgtcaaacgc
      481 tttgaggaaa tggtggcgca ggcgcagcag agcgccgggg c
//
LOCUS       DAJTQY010000289          521 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.299, whole genome shotgun sequence.
ACCESSION   DAJTQY010000289 DAJTQY010000000
VERSION     DAJTQY010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..521
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.299"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            20..304
                     /locus_tag="OO349_004177"
     CDS             20..304
                     /locus_tag="OO349_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001436860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5869024.1"
                     /translation="MVKISPDQKNGQGNRRFPEGAPYYPAFLYYGVRHSAYGMDKKDK
                     RQKKHHSSTFRLAPRNNFMADVIERRRHWTQELLSRELQRSLRPVKRKHK"
     gene            301..>521
                     /locus_tag="OO349_004178"
     CDS             301..>521
                     /locus_tag="OO349_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5869025.1"
                     /translation="MKLTTIIAALRERCPRFEERVGGAAQFKAIPDAGKLRLPAAYVV
                     PSDDAPGEQKSQTDYWQDLTEGFSVIVVL"
BASE COUNT          117 a          127 c          153 g          124 t
ORIGIN      
        1 gacgcgtcgc cccggactga tggtgaaaat ttcccctgac cagaaaaacg ggcaggggaa
       61 tcgccgtttt cctgaaggtg ctccttatta tccggcgttt ctgtattacg gcgttcgcca
      121 ttcagcgtat ggaatggata aaaaggataa gcggcagaag aagcaccatt cttcgacttt
      181 ccggctggcc ccgcgtaata actttatggc tgacgttatt gagcgtcggc gtcactggac
      241 gcaggaactg ttgtcccgtg agctgcagcg ttcgttacgt ccggtaaaaa ggaagcataa
      301 atgaaactga caacgataat tgcggcactg cgtgagcgat gcccgcgttt tgaggagcgt
      361 gttggtggcg cggcgcagtt taaggcgatc cctgatgccg gaaaacttcg cctgcctgct
      421 gcctatgtgg ttccctctga cgatgcgccg ggggaacaaa aatcacagac cgattactgg
      481 caggatttga ctgaaggctt ctccgtgatt gtggtgctca g
//
LOCUS       DAJTQY010000290          520 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.300, whole genome shotgun sequence.
ACCESSION   DAJTQY010000290 DAJTQY010000000
VERSION     DAJTQY010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..520
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.300"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            <1..>520
                     /locus_tag="OO349_004179"
     CDS             <1..>520
                     /locus_tag="OO349_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5869026.1"
                     /translation="HDKDVRRPTEMTQEALRRIAELYDIEAEIRGSPAEERLAVRKAR
                     SVQLMQSLYDWIQLQRKTLSKHAEMAKAFDYILNHWNALNEFCRDGWVEIDNNIGENA
                     LRSVAVGRKNYLFFGSDKGGESAAIIYSLLVTCKQNEVEPEDWLREVIEKLNDWPSNQ
                     VHELLPWNFSSVK"
BASE COUNT          150 a          111 c          159 g          100 t
ORIGIN      
        1 ccatgacaag gatgtgcgcc gtccgacaga aatgactcag gaagcgctca gacggatagc
       61 agagttatac gacatagaag cggagatacg tggcagtccg gcagaggaac ggcttgcagt
      121 cagaaaagcc agaagcgtcc agttgatgca gtcgttgtac gactggatac agttgcagag
      181 gaaaacgctg tcgaaacatg cggagatggc gaaggcgttc gactatatcc tgaatcactg
      241 gaatgcgctg aacgagttct gtcgtgacgg ctgggtggaa atagacaaca acatcggtga
      301 aaacgcgtta cgatcggtgg cggttggaag aaaaaattat ctctttttcg gctcagacaa
      361 gggaggagaa agtgcggcga tcatctacag tctgctggtc acctgcaaac agaacgaagt
      421 ggagccggag gactggttgc gcgaagtgat cgagaagctc aatgactggc cgtcgaacca
      481 agtgcatgaa ctgctgccct ggaacttctc gtctgtaaaa
//
LOCUS       DAJTQY010000291          510 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.301, whole genome shotgun sequence.
ACCESSION   DAJTQY010000291 DAJTQY010000000
VERSION     DAJTQY010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..510
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.301"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            <1..276
                     /locus_tag="OO349_004180"
     CDS             <1..276
                     /locus_tag="OO349_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR5869027.1"
                     /translation="SQNPTMTRQKLLPLVLQWLMEGETVVSELEKPSKSKKVSAIKVV
                     KPSDWDSLPDTDLRYIYSQRQPEKTMHERLKGKGVIVDMASLFKQAG"
     gene            279..>510
                     /locus_tag="OO349_004181"
     CDS             279..>510
                     /locus_tag="OO349_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator"
                     /protein_id="HCR5869028.1"
                     /translation="MRNFPVPYSNELIYSTIARAGVYQGIVSPKQLLDEVYGNRKVVA
                     TLGLPSHLGVIARHLHQTGRYAVQQLIYEHTLF"
BASE COUNT          151 a          102 c          127 g          130 t
ORIGIN      
        1 agccagaatc caacgatgac aagacaaaag ttactgcctc ttgttttgca gtggttgatg
       61 gaaggcgaaa cggtagtgtc agaactagaa aagccctcca agagtaaaaa ggtttcggct
      121 ataaaggtag tcaagcccag cgactgggat agcttgcctg atacggattt acgttatatc
      181 tattcacaac gccaacctga aaaaaccatg catgaacggt taaaagggaa aggggtaata
      241 gtggatatgg cgagcttatt taaacaagca ggttagccat gagaaacttt cctgttccgt
      301 actcgaatga gctgatttat agcactattg cacgggcagg cgtttatcaa gggattgtta
      361 gtcctaagca gctgttggat gaggtgtatg gcaaccgcaa ggtggtcgct accttaggtc
      421 tgccctcgca tttaggtgtg atagcaagac atctacatca aacaggacgt tacgctgttc
      481 agcagcttat ttatgagcat accttattcc
//
LOCUS       DAJTQY010000292          506 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.302, whole genome shotgun sequence.
ACCESSION   DAJTQY010000292 DAJTQY010000000
VERSION     DAJTQY010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..506
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.302"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            <1..>506
                     /locus_tag="OO349_004182"
     CDS             <1..>506
                     /locus_tag="OO349_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS911 family transposase"
                     /protein_id="HCR5869029.1"
                     /translation="KELGLVSCQQPTHRYKRGGHEHVAIPNYLERQFAVTEPNQVWCG
                     DVTYIWTGKRWAYLAVVLDLFARKPVGWAMSFSPDSRLTMKALEMAWETRGKPGGVMF
                     HSDQGSHYTSRQFRQLLWRYQIRQSMSRRGNCWDNSPMERFFRSLKNEWMPVVGYVSF
                     SEAAHAIT"
BASE COUNT          120 a          123 c          161 g          102 t
ORIGIN      
        1 aaagagctgg ggctggtcag ctgtcagcag ccgactcacc ggtataaacg tggtggtcat
       61 gaacatgttg ctatccctaa ctaccttgaa aggcagttcg ccgtgaccga gccaaatcag
      121 gtgtggtgcg gtgatgtgac ctatatctgg acgggtaagc gctgggcgta cctcgccgtt
      181 gttctcgacc tgttcgcaag aaaaccagtg ggctgggcca tgtcgttctc gccggacagc
      241 aggctcacca tgaaagcgct ggaaatggca tgggaaaccc gtggtaagcc cggcggggtg
      301 atgttccaca gcgatcaggg cagtcattat acgagcaggc agttccggca gttattgtgg
      361 cgataccaga tcagacagag tatgagccgg cgcggaaact gctgggataa cagcccaatg
      421 gaacgcttct tcaggagtct gaagaacgaa tggatgccgg tggtgggtta cgtaagcttc
      481 agcgaggcag ctcacgccat aacgga
//
LOCUS       DAJTQY010000293          483 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.303, whole genome shotgun sequence.
ACCESSION   DAJTQY010000293 DAJTQY010000000
VERSION     DAJTQY010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..483
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.303"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            <1..>483
                     /locus_tag="OO349_004183"
     CDS             <1..>483
                     /locus_tag="OO349_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCR5869030.1"
                     /translation="IRHIIIGNEGALCYITEVTVKIFKYTPENNLFYGYILEDMKTGF
                     NILREIMVEGYRPSIARLYDAEDGTQHFTHFADGKCVLIFMAEGNPRIAKATGEGIAE
                     IVARYPQCQRVDSKLIETWFNNLNWGPDKVAAERVQILKTGNMGFTTEVSGCWSCIHE
                     I"
BASE COUNT          131 a          124 c          122 g          106 t
ORIGIN      
        1 attcgtcaca tcatcatcgg caacgaaggt gcattgtgct atatcactga agtaacagtg
       61 aaaatcttta aatacacccc ggaaaacaac ctcttctacg gctatatcct ggaagacatg
      121 aaaaccggct tcaacatcct gcgtgaaatc atggtggaag ggtatcgtcc gtcgatcgct
      181 cgtttgtatg acgctgaaga tggcacccaa cacttcaccc attttgccga cggaaaatgt
      241 gtgctgatct ttatggctga aggtaaccct cgcattgcga aggcgacggg cgaagggatt
      301 gcggaaatcg ttgcccgcta cccgcagtgc cagcgcgtgg acagcaaact gattgaaacc
      361 tggtttaaca acctgaactg gggaccggat aaagtggctg ccgaacgtgt gcagatcctc
      421 aaaaccggca acatgggctt taccaccgaa gtgtccggct gctggagctg catccacgaa
      481 atc
//
LOCUS       DAJTQY010000294          451 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.304, whole genome shotgun sequence.
ACCESSION   DAJTQY010000294 DAJTQY010000000
VERSION     DAJTQY010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.304"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            1..451
                     /locus_tag="OO349_004184"
                     /pseudo
     CDS             1..451
                     /locus_tag="OO349_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          138 a          115 c          111 g           87 t
ORIGIN      
        1 gttgttgacc agaaatacac ggtggcagat gccgccaaag ctatggatgt tggcctttcc
       61 acaatgacaa gatgggtcaa acaactgcgt gatgagcgtc agggcaaaac accaaaagcc
      121 tcccccatta ccccggaaca aattgaaatc cgtgagctca ggaaaaagct acaacgcatt
      181 gaaatggaga atgaaatatt aaaaaaggct accgcgctct tgatgtcaga ctccctgaac
      241 agttctcgat aatcgggaaa ctcagagcgc attatcctgt ggtcacactc tgccatgtgt
      301 tcggggttca tcgcagcagc tacagatact ggaaaaaccg tcctgaaaaa ccagacggca
      361 gacgggctgt attacgcagt caggtacttg agctacatgg catcagccac ggttcggccg
      421 gagcaagaag catcgccaca atggcaaccc g
//
LOCUS       DAJTQY010000295          435 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.305, whole genome shotgun sequence.
ACCESSION   DAJTQY010000295 DAJTQY010000000
VERSION     DAJTQY010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..435
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.305"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
BASE COUNT          123 a           86 c           82 g          144 t
ORIGIN      
        1 cccataatgt cacttaccct catcggtaaa aaaattgcct ttcgaccatt acgatacatt
       61 attgattttg gggtttcagg gaagtaatcc atttcgacta taactgacag gtcatcacga
      121 tgtatgactg cgtatttgct actaaatagt ttctttattt tttccacgat gcccccaggt
      181 ttataagtac aaacggttat atccacatag agacaaaaat attaatctga taaatattta
      241 tttcacgccg tatatttgat tgtttaatgt gcaagtacaa tgacttttat tttttgttgt
      301 gtatataatc aaatatatgg ttatttttca ccctgcgtat tcagcacgca acaaaaaacc
      361 cgccgaagcg ggtttagtgc gggtgcgttg aggatgcctg acacatgaga ggtggcgagg
      421 gatttctccc ccgcc
//
LOCUS       DAJTQY010000296          422 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.306, whole genome shotgun sequence.
ACCESSION   DAJTQY010000296 DAJTQY010000000
VERSION     DAJTQY010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..422
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.306"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            1..422
                     /locus_tag="OO349_004185"
                     /pseudo
     CDS             1..422
                     /locus_tag="OO349_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          102 a          117 c          124 g           79 t
ORIGIN      
        1 gaacgtgaac tggagatgtc taccgagatt gcacgtctca aacgccagct ggcagaacgg
       61 gatgaagagc tggctatcct ccaaaaggcc gcgacatact tcgcgaagcg cctgaaatga
      121 agtatgtctt tattgaaaaa catcaggctg agttcagcat caaagcaatg tgccgcgtgc
      181 tccgggtggc ccgcagcggc tggtatacgt ggtgtcagcg gcggacaagg ataagcacgc
      241 gtcagcagtt ccgccaacac tgcgacagcg ttgtcctcgc ggcttttacc cggtcaaaac
      301 agcgttacgg tgccccacgc ctgacggatg aactgcgtgc tcagggttac ccctttaacg
      361 taaaaaccgt ggcggcaagc ctgcgccgtc agggactgag ggcaaaggcc tcccggaagt
      421 tc
//
LOCUS       DAJTQY010000297          407 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.307, whole genome shotgun sequence.
ACCESSION   DAJTQY010000297 DAJTQY010000000
VERSION     DAJTQY010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..407
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.307"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            <1..92
                     /locus_tag="OO349_004186"
     CDS             <1..92
                     /locus_tag="OO349_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR5869031.1"
                     /translation="HATGGDRVATSQTDRTAWHPDITRDKTRE"
     gene            89..>407
                     /locus_tag="OO349_004187"
     CDS             89..>407
                     /locus_tag="OO349_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCR5869032.1"
                     /translation="MNNELPDDIELLKAMLRKQQSRLRQYACQVAGYEQEIERLKAQL
                     DRLRRMLFGQSSEKKRHKLENQIRQAEKRLSELENRLNTARNLLEDASSVTDSPDTSP
                     PSEN"
BASE COUNT          126 a          100 c          106 g           75 t
ORIGIN      
        1 tccatgctac tggaggggat cgcgtggcaa catcccaaac ggacagaacg gcctggcatc
       61 cggatataac ccgtgataaa acaagggaat gaacaatgaa ctccccgatg atattgagct
      121 gcttaaagcc atgttgcgta agcaacagag tcggcttcga cagtatgcct gtcaggtcgc
      181 gggctatgag caggaaattg aacggctgaa agcgcaactc gacaggttgc gtcgtatgtt
      241 gttcggccag agttcagaga aaaagcgtca taagcttgaa aatcagatcc gacaggcaga
      301 aaaacgactg tcggaactgg aaaaccggct gaacacagcc agaaatcttc tggaagatgc
      361 atcgtcagtc acagattcac ctgacaccag tcccccgtca gaaaacc
//
LOCUS       DAJTQY010000298          388 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.309, whole genome shotgun sequence.
ACCESSION   DAJTQY010000298 DAJTQY010000000
VERSION     DAJTQY010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..388
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.309"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(<1..>388)
                     /locus_tag="OO349_004188"
     CDS             complement(<1..>388)
                     /locus_tag="OO349_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YdaU family protein"
                     /protein_id="HCR5869033.1"
                     /translation="RVASYGAIMAALPYMQLYIADYLADTMHLSAEEHGAYLLLMFNY
                     WQTGKPIPKNRLAKIARLTNERWVDVEPSLREFFCDNGDEWMHLRIEEDLASVREKLT
                     KKSAAGKASVQARRSRKEADVQTKQER"
BASE COUNT           84 a           99 c           79 g          126 t
ORIGIN      
        1 tctctcttgt tttgtttgaa catctgcttc ctttctgctt cttctggcct gaacagatgc
       61 ttttcctgcg gctgattttt tggttaattt ttctctgaca gatgccagat cttcctcaat
      121 ccgaagatgc atccattcgt cgccgttatc acaaaaaaac tcccgcaagg atggttcaac
      181 atcaacccat cgctcgttag tcagacgggc aatttttgcc aacctgtttt tgggtattgg
      241 ctttcctgtt tgccagtaat tgaacatcag caacaaatac gcgccgtgct cctctgctga
      301 caaatgcatg gtgtcagcca ggtaatcagc tatgtacagt tgcatgtatg gtaatgcggc
      361 cataattgcc ccgtatgatg ctacccgg
//
LOCUS       DAJTQY010000299          364 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.310, whole genome shotgun sequence.
ACCESSION   DAJTQY010000299 DAJTQY010000000
VERSION     DAJTQY010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.310"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(<1..177)
                     /locus_tag="OO349_004189"
     CDS             complement(<1..177)
                     /locus_tag="OO349_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCR5869034.1"
                     /translation="MNKSLTVLPSGEYPTMSSLEMVDYINADRKSKAEAEGLAFPCKK
                     YRKLEHRSFMKKVPR"
BASE COUNT           83 a           90 c           90 g          101 t
ORIGIN      
        1 cctgggtact ttcttcatga aactgcggtg ttcgagtttg cgatatttct tacaggggaa
       61 cgcaagcccc tccgcctccg ctttggattt ccgatcagca ttaatgtaat ccaccatctc
      121 aagactgctc atggtcgggt attcgccaga aggaagaacg gttaatgatt tgttcatgtc
      181 ggtatttcct ttagaaagtt gagcctgttc gcacagaaaa gccgtccccg agaggtcctc
      241 acctatacgg cagttctcag gctcagcttt ctgaaagact cgggattgtt acgcgctgcg
      301 atgcgcggtt tactgcggac atataaaagc cccgcaagtg cgaggctcat taaatggact
      361 ttgt
//
LOCUS       DAJTQY010000300          354 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.311, whole genome shotgun sequence.
ACCESSION   DAJTQY010000300 DAJTQY010000000
VERSION     DAJTQY010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..354
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.311"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            1..336
                     /locus_tag="OO349_004190"
                     /pseudo
     CDS             1..336
                     /locus_tag="OO349_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173292.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          127 a           75 c           82 g           70 t
ORIGIN      
        1 tgaattattt gccaacgacg gtacaccgac atggctaaaa gaaaagcgtg caaaagaaat
       61 cgcacttgat gaaattaact tgatgaaatt atcgagttgc tcaacaagca ccatgggggc
      121 accagtaacg acgccaaacg cgcaaaagct gatttgctgg aaaaaacatt cacctcccgc
      181 tcatgggaaa gaatcaaagg tatggactgg ccgacaatca aagctggtcg taatgcacta
      241 tggattgaac tggaaggcgt tgaatatgca ttcccggatc cacaaacaca aaacgaaacg
      301 caacaagcag gatacgatga gaatattccg gtgtaaaacc aagtgcattt tggg
//
LOCUS       DAJTQY010000301          344 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.312, whole genome shotgun sequence.
ACCESSION   DAJTQY010000301 DAJTQY010000000
VERSION     DAJTQY010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.312"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            <1..>344
                     /gene="tuf"
                     /locus_tag="OO349_004191"
     CDS             <1..>344
                     /gene="tuf"
                     /locus_tag="OO349_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR5869035.1"
                     /translation="AHVDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHIL
                     LGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKAL
                     EGDAEWEAKILEL"
BASE COUNT           77 a           88 c          105 g           74 t
ORIGIN      
        1 gcacacgtag actgcccggg gcacgccgac tatgttaaaa acatgatcac cggtgctgct
       61 cagatggacg gcgcgatcct ggtagttgct gcgactgacg gcccgatgcc gcagactcgt
      121 gagcacatcc tgctgggtcg tcaggtaggc gttccgtaca tcatcgtgtt cctgaacaaa
      181 tgcgacatgg ttgatgacga agagctgctg gaactggttg aaatggaagt tcgtgaactt
      241 ctgtctcagt acgacttccc gggcgacgac actccgatcg ttcgtggttc tgctctgaaa
      301 gcgctggaag gcgacgcaga gtgggaagcg aaaatcctgg aact
//
LOCUS       DAJTQY010000302          343 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.313, whole genome shotgun sequence.
ACCESSION   DAJTQY010000302 DAJTQY010000000
VERSION     DAJTQY010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..343
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.313"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
BASE COUNT           84 a           70 c           89 g          100 t
ORIGIN      
        1 ttgttagtct ttgcatgagc aagcggactg acacatttct ctttgttctc attcagaaaa
       61 tctcatcagt cggcgttcag accggtgttg tgctttatgg atacgttacg ctcctgatga
      121 cgtcatttga cgttcaacag catcacgggg ccgcacgaca tttcacgtca gttagtgcta
      181 tagctcagga acaaattttc ccaaattggg atatgctcgc aaattgctgg tgatgtgggg
      241 agaatctggt tgagttcggt agaattgatt tggaggcaga acgcttaaat cgtggcgtcc
      301 tgaaacgaaa aacggacctc cgtggaggtc cgttgatatg aat
//
LOCUS       DAJTQY010000303          342 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.314, whole genome shotgun sequence.
ACCESSION   DAJTQY010000303 DAJTQY010000000
VERSION     DAJTQY010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..342
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.314"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            101..>342
                     /locus_tag="OO349_004192"
     CDS             101..>342
                     /locus_tag="OO349_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005062103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5869036.1"
                     /translation="MAGVLEGRLSGGTAAEFCNSAVVALQAAGLDVCREYPVPERGDG
                     CGGRIDIVVTDRNGVRCGIELDRNSPRQKSLLKIGAV"
BASE COUNT           81 a           72 c          105 g           84 t
ORIGIN      
        1 tgccaacaca aaggcaacta ataaagatac agataaagat ctaaaaacag atctaaccca
       61 tcccaaaccc ttcccttccg gaagggagtt tcgggatttt gtggctggag tgcttgaggg
      121 gagattatct ggcggtactg cagcggaatt ttgtaattct gcggtggttg cgttgcaggc
      181 tgctggcctg gatgtctgtc gtgagtatcc ggtgccagag cgtggtgacg gttgcggagg
      241 gcggattgat atcgtcgtga ctgacaggaa cggtgtccgg tgtgggatcg agcttgaccg
      301 aaattctccg cgacagaaat cactgctcaa aatcggtgct gt
//
LOCUS       DAJTQY010000304          338 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.315, whole genome shotgun sequence.
ACCESSION   DAJTQY010000304 DAJTQY010000000
VERSION     DAJTQY010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.315"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(<1..>338)
                     /locus_tag="OO349_004193"
     CDS             complement(<1..>338)
                     /locus_tag="OO349_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5869037.1"
                     /translation="SRDNLGLKSAATMEAQSDIYDRTKGRLAIPGAFGFGCAFLPEDV
                     IRFDTKSDFLAWVRNALPGEYSVAGPYGIIIPDTRFEGVLSIRWTDARPETTEPRYRA
                     KSLTFYGING"
BASE COUNT           76 a           96 c           88 g           78 t
ORIGIN      
        1 gccgttaatg ccgtaaaaag taagggattt ggctctgtac cgcggttctg ttgtctcagg
       61 gcgtgcatca gtccaccgga tgctgagcac cccttcaaac cgtgtgtcgg gtatgatgat
      121 gccgtagggg ccagcaacgg aatattcacc tggcagcgca ttccttaccc aggccaggaa
      181 atcactctta gtgtcaaaac ggataacatc ttcaggcaga aaagcacacc caaagccgaa
      241 tgcgccgggt atcgccagac ggccttttgt ccggtcgtaa atgtcgctct gtgcttccat
      301 cgtggccgca cttttcagcc ccagattatc ccgggact
//
LOCUS       DAJTQY010000305          335 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.316, whole genome shotgun sequence.
ACCESSION   DAJTQY010000305 DAJTQY010000000
VERSION     DAJTQY010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..335
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.316"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
BASE COUNT          102 a           53 c           59 g          121 t
ORIGIN      
        1 atgtcgtgtt tttaattgta ggtgaggtga tttttcatta aaaaatatgc gattatgatt
       61 attttgtaag aacacattca taatattcat aatgcttgtg aatagtctta tgaataattc
      121 aatcgggatg tgtttatctg cgttacatta atttttcgca atagttaatt attccgttaa
      181 ttatggtaat gatgaggcac aaagagaaaa acctgccatt ttcccctact ttcaatcctg
      241 tgataggatg tcactgatga tgttaatcac actgacctta cagaatgggc cttatgaacg
      301 catttgattc gcaaaccgaa gattcttcac ctgca
//
LOCUS       DAJTQY010000306          335 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.317, whole genome shotgun sequence.
ACCESSION   DAJTQY010000306 DAJTQY010000000
VERSION     DAJTQY010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..335
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.317"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            21..>335
                     /locus_tag="OO349_004194"
     CDS             21..>335
                     /locus_tag="OO349_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5869038.1"
                     /translation="MKHEEMNQRFNHLENEITELNKKLSALVSSEDENKRRDEHYAAI
                     YDYCHKVAHETFMKFLQEKFLPAALSEKEAAYLRPEYVITVNGAGEEEHKSDFIASAP
                     DKD"
BASE COUNT          108 a           61 c           87 g           79 t
ORIGIN      
        1 ctgcacgggg agagtaaata atgaagcatg aagaaatgaa tcagcgattc aatcacctgg
       61 aaaatgaaat cactgagctt aataaaaaat tgtcggcgtt ggtgagttct gaagatgaaa
      121 acaaacgccg cgatgagcat tatgcggcga tttacgatta ctgccacaaa gtcgctcacg
      181 agacttttat gaagtttttg caggaaaagt ttttaccggc cgcattgtca gaaaaagagg
      241 cggcttacct gcggcctgag tacgtcatta cggttaacgg tgccggagag gaagagcata
      301 aaagtgattt tattgcgtct gcaccggata aagac
//
LOCUS       DAJTQY010000307          332 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.319, whole genome shotgun sequence.
ACCESSION   DAJTQY010000307 DAJTQY010000000
VERSION     DAJTQY010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.319"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            <1..>332
                     /locus_tag="OO349_004195"
     CDS             <1..>332
                     /locus_tag="OO349_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HCR5869039.1"
                     /translation="IYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKTGNM
                     FNQLLASKKLTKTAVNEALRRMKKAGIEKPELEAFLREMINGKQKTWLAHCTDAEALC
                     IDRVISEV"
BASE COUNT          102 a           63 c           99 g           68 t
ORIGIN      
        1 gatttaccca acaaatacag gcaaaagcgg ggaacacctt cgtctcacca cgctggaaag
       61 tgtctggatt cagggaaaac tgcgcatgtg ggggcgctgg tcgtatattg gcggcggtaa
      121 gacgggaaat atgtttaacc agttgctggc atccaaaaaa ttgacgaaaa cagccgtcaa
      181 tgaagccctg cgcaggatga aaaaagcggg aatagagaaa cctgagctgg aagcgttttt
      241 gcgagagatg atcaatggca agcaaaagac ctggctggcg cattgtactg atgcagaggc
      301 gttatgtatt gatagagtca taagtgaggt gc
//
LOCUS       DAJTQY010000308          318 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.321, whole genome shotgun sequence.
ACCESSION   DAJTQY010000308 DAJTQY010000000
VERSION     DAJTQY010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..318
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.321"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            <1..>318
                     /locus_tag="OO349_004196"
     CDS             <1..>318
                     /locus_tag="OO349_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HCR5869040.1"
                     /translation="RERKLVSGFVDYLCESVGREHTPALSDAAEQYLNTVATCRTRDT
                     ALLKSFDAFREWVTIQAGFYTEHIYPDGSRGRRAKSIAFANMDETEFQQVYKSVLNVL
                     WNWI"
BASE COUNT           83 a           72 c           85 g           78 t
ORIGIN      
        1 cgcgaacgaa aactggtatc aggcttcgtt gattacctgt gcgaatcagt aggtcgggaa
       61 cacactccag ccctgagtga tgccgcagag caatacctta atacagttgc gacatgcaga
      121 acccgggata cggcattgct aaagtcgttt gacgctttcc gcgagtgggt aaccattcag
      181 gccggatttt acaccgagca tatttatcct gatggtagtc gtgggcgcag ggcaaaatct
      241 atcgcatttg cgaacatgga cgaaaccgag tttcagcagg tttataaatc tgtactgaat
      301 gtgctgtgga actggatc
//
LOCUS       DAJTQY010000309          310 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.323, whole genome shotgun sequence.
ACCESSION   DAJTQY010000309 DAJTQY010000000
VERSION     DAJTQY010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..310
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.323"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(71..277)
                     /locus_tag="OO349_004197"
     CDS             complement(71..277)
                     /locus_tag="OO349_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5869041.1"
                     /translation="MTFLIPIYKDDDFDSDTVGFTFAFKMPRGQFFVDVKENGNIRAG
                     VNVNGESGVTYENCKLNMKDINDD"
BASE COUNT          101 a           67 c           43 g           99 t
ORIGIN      
        1 agaaggtaaa gaaggtaagt ccatactgtc tccactataa aaaaaccgcc aatttctggc
       61 ggcttatata ttaatcatca ttaatatctt tcatattcaa tttgcaattt tcgtaggtca
      121 ctcccgactc gccattaaca ttaactccag ctctgatatt cccgttttct tttacatcta
      181 caaaaaactg gcctcttggc atcttgaacg caaatgtaaa accaacagta tctgaatcaa
      241 aatcatcatc tttatatatt gggatcaaaa atgtcatcgt cgctggtgat gcattgtttg
      301 caaaatcaaa
//
LOCUS       DAJTQY010000310          310 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.324, whole genome shotgun sequence.
ACCESSION   DAJTQY010000310 DAJTQY010000000
VERSION     DAJTQY010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..310
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.324"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
BASE COUNT          122 a           43 c           63 g           82 t
ORIGIN      
        1 tattctcaaa atatagtaga aacgaggtat gtttctggtg gaaaggacag tgggattaaa
       61 aagtaagaat tgataaaaaa aacgccagca acacatagtg ccggcggagg gaatacccca
      121 tggagaaaat gtgatgccta gagcatcatg aatatataat taaaaatagt tagcgttgtc
      181 aatactttca caaaaataat tttcgtagta tagaaagata tttttatgca tgacctacct
      241 gaatttgctc cgggtagagg ttataaataa aaattgaatc acgacaaaca caatattcat
      301 agtatggcga
//
LOCUS       DAJTQY010000311          297 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.denovo.325, whole genome shotgun sequence.
ACCESSION   DAJTQY010000311 DAJTQY010000000
VERSION     DAJTQY010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..297
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.denovo.325"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(52..297)
                     /locus_tag="OO349_004198"
                     /pseudo
     CDS             complement(52..297)
                     /locus_tag="OO349_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105095.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
BASE COUNT           76 a           70 c           78 g           73 t
ORIGIN      
        1 gctgcttaaa aaaagaacaa atggtagtgt ccgctattgc cagtacacct cagccttaat
       61 tgcatcagtc aggcggagca gggcggcatt atccagagtt gtcggttctg ccatgccgcg
      121 tatggccaac agcagattct ggacatagct gttttcctga tccatctcaa gagcagtaat
      181 gtgtttgcgt tgttcacggg tggcatgatg caggtatttc cgatccccgg cagcatacgt
      241 aaaaatgtgc agaagacgct gtgtgaaccg caaagtggct acagagactt cgcaatc
//
LOCUS       DAJTQY010000312         5250 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.guided.179, whole genome shotgun sequence.
ACCESSION   DAJTQY010000312 DAJTQY010000000
VERSION     DAJTQY010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5250
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.guided.179"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            720..1535
                     /gene="sul2"
                     /locus_tag="OO349_004199"
     CDS             720..1535
                     /gene="sul2"
                     /locus_tag="OO349_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCR5869042.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            1596..2399
                     /locus_tag="OO349_004200"
                     /note="aph(3'')-Ib"
     CDS             1596..2399
                     /locus_tag="OO349_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HCR5869043.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            2399..3235
                     /locus_tag="OO349_004201"
     CDS             2399..3235
                     /locus_tag="OO349_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HCR5869044.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(3342..3746)
                     /locus_tag="OO349_004202"
                     /pseudo
     CDS             complement(3342..3746)
                     /locus_tag="OO349_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(3790..4179)
                     /locus_tag="OO349_004203"
     CDS             complement(3790..4179)
                     /locus_tag="OO349_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5869045.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            4397..4837
                     /locus_tag="OO349_004204"
     CDS             4397..4837
                     /locus_tag="OO349_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HCR5869046.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
BASE COUNT         1240 a         1410 c         1384 g         1216 t
ORIGIN      
        1 cctttcggtt taccggtgtc attccgctgt tatggccgcg gtttctcatt ccacgcctga
       61 cactcagttc cgtgaaggca gttcgctcca agctggactg tatgcacgaa ccccccgttc
      121 agtccgaccg ctgcgccttg tccggtaact atcaacttga gtccaacccg gaaagacacg
      181 acaaaacgcc actggcagca gccattagta acaggagtgc acgagaagat agattaagag
      241 gtcttgaagt agaggcctga ctgcggctac actgaaagaa cagttttggt aatggcggtc
      301 tcgtgaaccc tgttaccggg ttaagcagtt ccccaactga cttaaccttc gaacaaaccg
      361 cctccccagg cggtttgttc gtttacagag caagagatta cgattcattg aaaaggatct
      421 caagaagatc ctttgtagat tactatcaat taatcttccg aacatgacac cagtcactgt
      481 tgattacggt taaaccgctc cgtaacaggt ttccctcgta ttggtgactt gcagcaccaa
      541 agaagcaaaa aagataaata tatctactta ttgagagatg atatatttat ttccaaaatg
      601 ctacagatac atcatcatga aacggatcac cgcaaacagg ttactcgcct ggggattccc
      661 tttcgacccg agcatccgta tgagactcat gctcgattat tattattata gaagccccca
      721 tgaataaatc gctcatcatt ttcggcatcg tcaacataac ctcggacagt ttctccgatg
      781 gaggccggta tctggcgcca gacgcagcca ttgcgcaggc gcgtaagctg atggccgagg
      841 gggcagatgt gatcgacctc ggtccggcat ccagcaatcc cgacgccgcg cctgtttcgt
      901 ccgacacaga aatcgcgcgt atcgcgccgg tgctggacgc gctcaaggca gatggcattc
      961 ccgtctcgct cgacagttat caacccgcga cgcaagccta tgccttgtcg cgtggtgtgg
     1021 cctatctcaa tgatattcgc ggttttccag acgctgcgtt ctatccgcaa ttggcgaaat
     1081 catctgccaa actcgtcgtt atgcattcgg tgcaagacgg gcaggcagat cggcgcgagg
     1141 cacccgctgg cgacatcatg gatcacattg cggcgttctt tgacgcgcgc atcgcggcgc
     1201 tgacgggtgc cggtatcaaa cgcaaccgcc ttgtccttga tcccggcatg gggttttttc
     1261 tgggggctgc tcccgaaacc tcgctctcgg tgctggcgcg gttcgatgaa ttgcggctgc
     1321 gcttcgattt gccggtgctt ctgtctgttt cgcgcaaatc ctttctgcgc gcgctcacag
     1381 gccgtggtcc gggggatgtc ggggccgcga cactcgctgc agagcttgcc gccgccgcag
     1441 gtggagctga cttcatccgc acacacgagc cgcgcccctt gcgcgacggg ctggcggtat
     1501 tggcggcgct gaaagaaacc gcaagaattc gttaactgca cattcgggat atttctctat
     1561 attcgcgctt catcagaaaa ctgaaggaac ctccattgaa tcgaactaat attttttttg
     1621 gtgaatcgca ttctgactgg ttgcctgtca gaggcggaga atctggtgat tttgtttttc
     1681 gacgtggtga cgggcatgcc ttcgcgaaaa tcgcacctgc ttcccgccgc ggtgagctcg
     1741 ctggagagcg tgaccgcctc atttggctca aaggtcgagg tgtggcttgc cccgaggtca
     1801 tcaactggca ggaggaacag gagggtgcat gcttggtgat aacggcaatt ccgggagtac
     1861 cggcggctga tctgtctgga gcggatttgc tcaaagcgtg gccgtcaatg gggcagcaac
     1921 ttggcgctgt tcacagccta tcggttgatc aatgtccgtt tgagcgcagg ctgtcgcgaa
     1981 tgttcggacg cgccgttgat gtggtgtccc gcaatgccgt caatcccgac ttcttaccgg
     2041 acgaggacaa gagtacgccg cagctcgatc ttttggctcg tgtcgaacga gagctaccgg
     2101 tgcggctcga ccaagagcgc accgatatgg ttgtttgcca tggtgatccc tgcatgccga
     2161 acttcatggt ggaccctaaa actcttcaat gcacgggtct gatcgacctt gggcggctcg
     2221 gaacagcaga tcgctatgcc gatttggcac tcatgattgc taacgccgaa gagaactggg
     2281 cagcgccaga tgaagcagag cgcgccttcg ctgtcctatt caatgtattg gggatcgaag
     2341 cccccgaccg cgaacgcctt gccttctatc tgcgattgga ccctctgact tggggttgat
     2401 gttcatgccg cctgtttttc ctgctcattg gcacgtttcg caacctgttc tcattgcgga
     2461 caccttttcc agcctcgttt ggaaagtttc attgccagac gggactcctg caatcgtcaa
     2521 gggattgaaa cctatagaag acattgctga tgaactgcgc ggggccgact atctggtatg
     2581 gcgcaatggg aggggagcag tccggttgct cggtcgtgag aacaatctga tgttgctcga
     2641 atatgccggg gagcgaatgc tctctcacat cgttgccgag cacggcgact accaggcgac
     2701 cgaaattgca gcggaactaa tggcgaagct gtatgccgca tctgaggaac ccctgccttc
     2761 tgcccttctc ccgatccggg atcgctttgc agctttgttt cagcgggcgc gcgatgatca
     2821 aaacgcaggt tgtcaaactg actacgtcca cgcggcgatt atagccgatc aaatgatgag
     2881 caatgcctcg gaactgcgtg ggctacatgg cgatctgcat catgaaaaca tcatgttctc
     2941 cagtcgcggc tggctggtga tagatcccgt cggtctggtc ggtgaagtgg gctttggcgc
     3001 cgccaatatg ttctacgatc cggctgacag agacgacctt tgtctcgatc ctagacgcat
     3061 tgcacagatg gcggacgcat tctctcgtgc gctggacgtc gatccgcgtc gcctgctcga
     3121 ccaggcgtac gcttatgggt gcctttccgc agcttggaac gcggatggag aagaggagca
     3181 acgcgatcta gctatcgcgg ccgcgatcaa gcaggtgcga cagacgtcat actagatatc
     3241 aagcgacttc tcctatcccc tgggaacaca tcaatctcac cggagaatat cgctggccaa
     3301 agccttagcg taggattccg ccccttcccg caaacgaccc caaacaggaa acgcagctga
     3361 aacgggaagc tcaacaccca ctgacgcatg ggttgttcag gcagtacttc atcaaccagc
     3421 aaggcggcac tttcggccat ccgccgcgcc ccacagctcg ggcagaaacc gcgacgctta
     3481 cagctgaaag cgaccaggtg ctcggcgtgg caagactcgc agcgaacccg tagaaagcca
     3541 tgctccagcc gcccgcattg gagaaattct tcaaattccc gttgcacata gcccggcaat
     3601 tcctttccct gctctgccat aagcgcagcg aatgccgggt aatactcgtc aacgatctga
     3661 tagagaaggg tttgctcggg tcggtggctc tggtaacgac cagtatcccg atcccggctg
     3721 gccgtcctgg ccgccacatg aggcatgttc cgcgtccttg caatactgtg tttacataca
     3781 gtctatcgct tagcggaaag ttcttttacc ctcagccgaa atgcctgccg ttgctagaca
     3841 ttgccagcca gtgcccgtca ctcccactct tacattgttc gcagtatcta ggtacatgtt
     3901 attgcgtgca ccttcatgat gtataatgtt attaatgaca gcagaaagat cgacagaatt
     3961 aatattcttc gtacctggga cagttatcgg gtgtcggcca gtgatgagac caaaggatgc
     4021 agtataagat gaaccactac tattatcatg gccaccatta ccaccaccgt taaaactacc
     4081 tcccgaactt ccagagccgc tatgaccatc attgtagggg tcatttggtc ccatgtgacc
     4141 aaaaccaccg acagtaccgc tatttaactc tgtactcata aaattatcct ctttgacttt
     4201 taaaaacagc aaaaactgta tttataacaa tgtgtttata tacagtattc gaaatactac
     4261 gcccgtcaaa accacaccgt caacaggttt tttgtgtatt tttcacgcct cacgggcagc
     4321 ctcagacctt ttgtgttaaa acagtaatat gttctgctgt tacaatacgg tacacacagg
     4381 agacactgtc acttttatgg catggcacac agttcaagag acactgaaat taacaggaaa
     4441 gtcgcgctcg caattatatc gtgacatggc taaaggcctt gtcagctatc gcacaggaaa
     4501 aaatgatcgc cgggagttcg agaccagtga actgattcgg gcctatggcg agctgcgcac
     4561 ttctgagaca ccaaaaaggc acaaggaggg acactccaaa tctgtcgtta gccaggcgga
     4621 aaatgctcag ctagacgtca ttcagcagaa actggacgct ctgcagcaga ctgttaccct
     4681 aatgcttgaa gacaagatgg cgcgggaagc agaaaaccgc ctgcgaaatg aggagaggga
     4741 acaactacag gccgaaatta gccgtctggc tgacgcgctg gcacaggaaa aaaagcgggg
     4801 attctggtcg cggctgtttc gctccggttc agagtaacag tgcaggtgac tctgatttac
     4861 cataacatct gttatcagca ccgcctgaga ggccctcagg gcgtttctga cgggaaaccg
     4921 gtaaaacagg caacaaacca cccgaaaacc cgccagaaac gcgcaggctc tgtttttacg
     4981 gcatgcatga gtatgcgcac aagtgcatag cgcggaaatt cacaccattc agccggggta
     5041 agtcctgttt ccggcgttct tcccatccga aaacggtgaa cggggacgca atttattgcg
     5101 tggccgtgaa gcgttgccgg cgcagccgga aaacccgctg tcagcacgtt caggaagggg
     5161 gtgatgtaca aatggcacag aggatgaaag tgacgtatga gctgaaggaa tgatgtaaaa
     5221 gtgatgtata agtgatgtaa aagtgatgta
//
LOCUS       DAJTQY010000313         4621 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.guided.191, whole genome shotgun sequence.
ACCESSION   DAJTQY010000313 DAJTQY010000000
VERSION     DAJTQY010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4621
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.guided.191"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            <1..107
                     /locus_tag="OO349_004205"
     CDS             <1..107
                     /locus_tag="OO349_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="in conjunction with FrdC acts to anchor the
                     catalytic components of the fumarate reductase to the
                     cytoplasmic membrane; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fumarate reductase subunit D"
                     /protein_id="HCR5869047.1"
                     /translation="AMHDLKIHVPAGKWVFYGLAAILTVVTLIGVVTI"
     gene            170..1303
                     /locus_tag="OO349_004206"
     CDS             170..1303
                     /locus_tag="OO349_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCR5869048.1"
                     /translation="MFKTTLCALLITASYSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            1392..1925
                     /gene="blc"
                     /locus_tag="OO349_004207"
     CDS             1392..1925
                     /gene="blc"
                     /locus_tag="OO349_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCR5869049.1"
                     /translation="MRLLPLVAAATAAFLVVACSFPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(1922..2239)
                     /gene="sugE"
                     /locus_tag="OO349_004208"
     CDS             complement(1922..2239)
                     /gene="sugE"
                     /locus_tag="OO349_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HCR5869050.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(2415..2561)
                     /gene="ecnB"
                     /locus_tag="OO349_004209"
     CDS             complement(2415..2561)
                     /gene="ecnB"
                     /locus_tag="OO349_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HCR5869051.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(2672..2797)
                     /gene="ecnA"
                     /locus_tag="OO349_004210"
     CDS             complement(2672..2797)
                     /gene="ecnA"
                     /locus_tag="OO349_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HCR5869052.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            complement(2849..3415)
                     /gene="efp"
                     /locus_tag="OO349_004211"
     CDS             complement(2849..3415)
                     /gene="efp"
                     /locus_tag="OO349_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCR5869053.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            3457..4485
                     /gene="epmB"
                     /locus_tag="OO349_004212"
     CDS             3457..4485
                     /gene="epmB"
                     /locus_tag="OO349_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HCR5869054.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKEHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         1173 a         1182 c         1166 g         1100 t
ORIGIN      
        1 acgcgatgca cgatctgaaa atccacgtgc ctgcgggcaa atgggttttc tacggtctgg
       61 ctgctatcct gacagttgtc acgctgattg gtgtcgttac aatctaacgc atcgccaatg
      121 taaatccggc ccgcctatgg cgggccgttt tgtatggaaa ccagacctta tgttcaaaac
      181 gacgctctgc gccttattaa ttaccgcctc ttactccaca tttgctgccc ctcaacaaat
      241 caacgatatt gtgcatcgca caattacccc gcttatagag caacaaaaga tccccggtat
      301 ggcggtggcg gtaatttatc agggtaaacc ttattacttt acctggggct atgcggacat
      361 cgccaaaaag cagcccgtca cacagcaaac gttgtttgag ttaggttcgg tcagcaaaac
      421 atttacgggc gtgcttggtg gcgacgctat tgctcgaggg gaaatcaagt taagcgatcc
      481 cgcaacaaaa tactggcctg aacttaccgc taaacagtgg aatgggatca cactattaca
      541 tcttgcgacc tacaccgctg gcggcctgcc attgcaggtg ccggatgaag tgaaatcctc
      601 aagcgacttg ctgcgcttct atcaaaactg gcagcctgca tgggctccag gaacacaacg
      661 tctgtatgcc aactccagta tcggtttgtt cggcgcactg gctgtgaagc cgtctggttt
      721 gagttttgag caggcgatgc aaactcgtgt cttccagcca ctcaaactca accatacgtg
      781 gattaatgta cctcccccag aagaaaagaa ttacgcctgg ggatatcgcg aaggtaaggc
      841 agtgcatgtt tcgccagggg cgttagatgc tgaagcttat ggtgtgaagt cgaccattga
      901 agatatggcc cgctgggtgc aaagcaattt accccccctt gatatcaatg agaaaacgct
      961 tcaacaaggg atacaactgg cacaatctcg ctactggcaa accggcgata tgtatcaggg
     1021 cctgggctgg gaaatgctgg actggccggt caatcctgac agcatcatta acggcagtga
     1081 caataaaatt gcactggcag cacgcccggt aaaagcgatt aagcccccaa ctcctgcagt
     1141 acgcgcatca tgggtacata aaacgggggc gaccggcgga tttggtagct atgtcgcgtt
     1201 tattccagaa aaagagctgg gtatcgtgat gctggctaac aaaaactatc ccaatccagc
     1261 gagagtcgcc gccgcctgtc agattctcaa cgctctacag taaaatttca tcgggtccga
     1321 attttcggac cttttctgcg cttttccttg ctgtcatcta cacttagaaa aaaaccagta
     1381 aggaaacatt tatgcgcctg ctccctctcg ttgccgcagc gacagctgca tttctggtcg
     1441 ttgcctgtag ttttcctacg ccgccgcgtg gcgtgaccgt agtgaataat ttcgacgcca
     1501 aacgttatct cggtacctgg tatgagattg cccgttttga tcaccgtttt gaacgtggac
     1561 tggaaaaagt caccgcaaca tacagcctgc gtgatgacgg cggcctgaat gtcattaata
     1621 aaggctataa ccctgacaga ggaatgtggc agcagagtga agggaaagcg tactttaccg
     1681 gcgcaccaac tcgcgctgcg ctgaaagtgt cattctttgg tcctttctat ggcggttata
     1741 acgttattgc actcgatcgg gaataccgcc atgcgctggt ttgcggcccg gaccgcgact
     1801 acctgtggat actctcccgc acgccaacca tttctgacga agtgaaacag gagatgctgg
     1861 cagtcgcgac ccgggaaggg tttgatgtca gtaagtttat ttgggtacag cagcccggta
     1921 gttagtgagt gctgagtttc agaccaataa tccccaatac gattaacgcc agactcgcca
     1981 gacgcatcgg gttagcggac tcaccgagca gcacaatgcc ggtgatggcc gcgccgactg
     2041 cgccaatacc cgtccacacg gcataagccg tccctaccgg taacgatttc atcgcccagg
     2101 caagtagcgc catactgact atcatcgccg tcacggtaat aacactcggc gtcaaacggc
     2161 taaagccgtg ggtatatttc aggccaacgg cccataccac ttccagcaga ccagcaataa
     2221 ctaagataat ccaggacata tcaggctccg gaaatttggg gccgtccccg gtgaaaagag
     2281 gcgtttgcag gtcgtcctgc aaagctgatg gttgaacgga tattttgcca ggtggcaggg
     2341 gaatttcaac tattaccttt atgacgatcg cagaaaaaac aaacggcacg acactgctgt
     2401 cgtaccgtta ttgcttattg ctgcgctttc gttgctgcgc cagaaatcgc gttaccgcca
     2461 tcagaaatgt cttcaccaac gccacgcgtg gtgttgcagg cagttaatac tgttgaaagc
     2521 accagaacag aaaagatcgc tgcaattgtc ttcttcacca taacgtcttc cttttaggta
     2581 atgttattta tgtttgccta tagcaaatta ataatagaca acatcgccaa aaatgatgga
     2641 aatctgatga tttttcggaa gagaagaaaa attagctggc agcgcgagag atggagttac
     2701 cgagatgttt gatgtcttcg ccgaaaccgc aagcggtgtt acagcccgtg agcagcgtgc
     2761 tggcaagcaa aacaagaacg ataaggcgtt tcatcatctc tgacctgaat aagtgatggt
     2821 gcagcctgca ggccgcacca caaccgcatt acttcacgcg agagacgtat tcaccagagc
     2881 gggtatccac tttgatgact tcgccgattt gtacgaacag cggaacttta accacagcgc
     2941 cagtagacag ggtagccggt ttgccgccag tacctgcggt atcacctttc aggcccggat
     3001 cggtatcaac gatttccagt tcaacgaagt tcggcggagt aacggagatc ggctgaccat
     3061 tccacagagt tacgatacac tctgcctgat ccagcagcca tttagcattg tcgccaattg
     3121 cttttgcatc agcagacagc tgctcgaaag tttcgttgtt catgaagtgc cagaactcac
     3181 cgtcgttgta caggtaagtc aggttcatat cgacaacatc agcgccttca gcggaatcag
     3241 tagatttgaa ggttttttct acgcgagtac cggtcagcag acgacgcagt ttaacgcgag
     3301 caaatgcctg gcctttaccc ggttttacga attcactcgc ttcaaccgcg taaggttcgc
     3361 cgtctaacat gattttaaga ccagcacgaa aatcgttgct atagtacgtt gccataaggc
     3421 cctctgaaat ttgttaattg gtagctaagc cacaaaatgg cgcatattgt aaccctaaat
     3481 accccatcca gagaagattg gttaacgcaa cttgccgatg ttgtgaccga tcctgatgaa
     3541 cttctgcgtc ttttgaatat agacgcggac gaaaaactgt tggccggacg cagcgccaaa
     3601 aagctgtttg ccctgcgtgt gccccgctca tttatcgatc gcatggagaa aggcaatccg
     3661 gacgatcctc ttttgcgtca ggtacttacc tcgcaagatg agtttgtcgt cgcgtccgga
     3721 ttctccaccg acccactgga agaacagcac agcgtagtgc ctggtttgtt gcataaatac
     3781 cacaaccggg cgcttttgct ggtcaaaggc ggctgcgcgg taaattgccg ctattgcttc
     3841 cgtcgccact tcccctatgc cgaaaatcag ggcaacaagc gtaactggca gactgcactt
     3901 gagtatgttg ctgcgcatcc ggaactggac gagatgattt tctccggcgg cgatccgctg
     3961 atggcgaaag agcacgagct ggactggttg ctcacacaac tggaagccat cccgcatatc
     4021 aaacgtctgc ggattcacag ccgtctgccg attgtgatcc cggcacgtat caccgaggcg
     4081 ctggttgaac gctttgcccg ttctacgctg caaatcttgc tggtgaatca catcaatcat
     4141 gccaatgagg tagatgaaac gttccgtcag gcgatggcta aattgcgccg tgtcggtgtc
     4201 accctgctta accagagcgt tctgttacgt ggtgtgaacg ataacgcaca aacgttggca
     4261 aacctgagta atgcgttgtt cgatgccggc gtgatgccgt attacctgca tgtgctagat
     4321 aaagtacagg gcgcggcgca ttttatggtg agtgatgacg aagcacggca gattatgcgt
     4381 gagttgctga cattggtgtc gggatatctg gtgccgaaac tggcgcgaga aattggcggc
     4441 gaacccagca aaacgccgct ggatctccag ctacgccagc agtaactgat taaacaggga
     4501 agttaaacat taaaattgcc tgatgcgcta cgcttatcag gcctacatcg tgcattgaaa
     4561 ttgaatggat ttgcaatgaa ctgtaggccg gataaggcgt ttacgccgca tccggcaaga
     4621 g
//
LOCUS       DAJTQY010000314         3434 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.guided.206, whole genome shotgun sequence.
ACCESSION   DAJTQY010000314 DAJTQY010000000
VERSION     DAJTQY010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3434
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.guided.206"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            1..243
                     /gene="prfH"
                     /locus_tag="OO349_004213"
                     /pseudo
     CDS             1..243
                     /gene="prfH"
                     /locus_tag="OO349_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="similar to release factor 1 and 2; internal stop;
                     incomplete; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(300..1757)
                     /gene="pepD"
                     /locus_tag="OO349_004214"
     CDS             complement(300..1757)
                     /gene="pepD"
                     /locus_tag="OO349_004214"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HCR5869055.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            2019..2477
                     /gene="gpt"
                     /locus_tag="OO349_004215"
     CDS             2019..2477
                     /gene="gpt"
                     /locus_tag="OO349_004215"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCR5869056.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            2569..>3434
                     /gene="frsA"
                     /locus_tag="OO349_004216"
     CDS             2569..>3434
                     /gene="frsA"
                     /locus_tag="OO349_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HCR5869057.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPKLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPR"
BASE COUNT          812 a          889 c          893 g          840 t
ORIGIN      
        1 aataaaaccg actcggcggt acgcgccacg catttggcat ccggtattag cgtgaaggtt
       61 cagtcagagc gcagccagca tgctaacaaa cggctggcgc gattgctgat tgcctggaag
      121 ctggatcagc agcaatagga aaatagcgcg gtgctgaaat cacagcggcg aatgttccat
      181 caccagattg aacgtggcaa cccgcgacga acgtttacag ggatggcttt tatcgaagga
      241 taatgaagga tgaaactgcc ggaaggcgat taaacgccat ccggcagcaa atcaaataat
      301 tacttcgccg gaatttcttt cagcagttca gtcagcagtg tccagtaatg acctacgctt
      361 tcgatgtgaa cttgctcatc cggagagtgt ggaccggtga tagttggccc gatagaaacc
      421 atgtccattt ccggatacgg ttttttgaac agaccacatt ccaggcccgc gtggataatc
      481 tggatgttcg gcgtcttgtt gaacaggcgc tgataggttt cacgtaccag atgcatcacc
      541 ggagaattag cgtccggctg ccagccagga tatgcgcctt tcgcttcggt tttcgcgcca
      601 gccagtttac ccagcgaatc cagcatgctc accacgtagt ctttaccgct gtcgatcagt
      661 gaacggatca ggcagtgaat ttctacgtta ttgtcagtca tggtcaccac accgacgttc
      721 agggaggttt cgaccacacc ttttgccacg tcggaattgc ggatcacacc gttcggggtg
      781 gcgttcagca gacgaataaa ggtatcgcga gatttcgcaa tcagggcagc tttatcgttc
      841 gctacagagt ccagcaacaa ggccagattt ttctctttct ctgccagctc gtttttcagg
      901 atctcctgat aggtattcac cagagatttc agtgcgtcga ctttatcagc tgcgacagca
      961 atggtcgcaa aggcttcacg cgggatggcg ttacgcagtg tgccgccgtt gaaatcgata
     1021 aggcgcaggt ccagttcttc tgcatgaccc gccaggaagc gcaccagcag tttgttggca
     1081 ttacccaggc caacgtggat ttcaccgccg gagtgaccgc ctttcagacc ttttaaggtt
     1141 aacttgaagg tttcaaaacc agctggaacc gcttcacgat ctaaatgcag gttggaggtg
     1201 aagtcgatac cccccgcaca acccatgtag atttcacctt cttcttcgga gtcggtgtta
     1261 atcagaatat cagcctgcaa ccagttgctc tgtaagccga acgcaccgtc cataccggct
     1321 tcttcggtca tggtcagcag cacttccagc gggccgtgaa ccacgttttc gtcagccaga
     1381 accgccagtg cagaggccat accaatgccg ttatccgcac ccagcgtggt gccgcgcgct
     1441 ttaacccatt cgccatcaat ataaggctgg ataggatctt tcgtgaagtc atgcacggtg
     1501 tcgttatttt tctgcggcac catatcgagg tgggcctgta agacgaccgg tttacgattt
     1561 tccatacctg cggtagcagg tttacgaatc aggatattac ctacctgatc gcgttcgaca
     1621 tggaaacctt tctcttttgc ccaaccaaca atgtattcag cgagttgctc ttcatgatag
     1681 gacgggtgag gaatagaaca gattttggca aaaatatccc acagcggctg tggagataat
     1741 tgagacagtt cagacacgtt aagtctcctt gtcgatcacc cgcaaaacag tattgcaggt
     1801 cacagggtta gcagaaaatg ttgtcaacac aagacaggct tgcgagatat gtattgagaa
     1861 taccacttta tcccgcgtca gggagaggca gtgcgtaaaa agacgcggac tcatgtgaaa
     1921 tactggtttt tagtgcgcca gatctctata atctcgcgca acctattttc ccctcgaaca
     1981 ctttttaagc cgtagataaa caggctggga cacttcacat gagcgaaaaa tacatcgtca
     2041 cctgggacat gttgcagatc catgcacgta aactcgcaag ccgactgatg ccttctgaac
     2101 aatggaaagg cattattgcc gtaagccgtg gcggtctggt accgggggcg ttactggcgc
     2161 gtgaactggg tattcgtcat gtcgataccg tttgtatttc cagctacgat cacgacaacc
     2221 agcgcgagct taaagtgctg aaacgcgcag aaggcgatgg cgaaggcttc atcgttattg
     2281 atgacctggt ggataccggt ggtactgctg ttgcgattcg tgaaatgtat ccaaaagcgc
     2341 actttgtcac catcttcgca aaaccggctg gtcgtccgct ggttgatgac tatgttgttg
     2401 atatcccgca agatacctgg atcgaacagc cgtgggatat gggcgtcgta ttcgtcccgc
     2461 caatctccgg tcgctaatct tttcaacgcc tggcattgcc gggcgttgtt ctttttaact
     2521 tcaggcaggt tacaatagtt tccagtaagt attctggagg ctgcatccat gacacaggca
     2581 aacctgagcg aaaccctgtt caaaccccgc tttaaacatc ctgaaacctc gacgctagtc
     2641 cgccgcttta atcacggcgc acaaccgcct gtgcagtcgg ctcttgatgg taaaaccatc
     2701 cctcactggt atcgcatgat taaccgtctg atgtggatct ggcgcggtat tgacccacgt
     2761 gaaatcctcg acgtccaggc acgtattgtg atgagcgatg ctgaacgtac cgacgatgat
     2821 ttatacgata ccgtgattgg ctaccgtggt ggcaactgga tttatgagtg ggccacccag
     2881 gcgatggtgt ggcaacaaaa agcctgtacg gaagaagatc cgaaactcag tggtcgacac
     2941 tggctgcatg cggctacgtt gtacaacatt gccgcctatc ctcatctgaa aggagatgac
     3001 ctggccgagc aagcgcaggc tttatcaaac cgcgcctatg aagaggccgc ccagcgtctg
     3061 ccgggcacga tgcggcagat ggagtttacc gtacccggcg gtgcgcccat caccggcttt
     3121 ttgcatatgc cgaaaggcga tggcccgttc ccgacagtat taatgtgtgg tggtctggat
     3181 gcgatgcaga cggactatta cagcctgtat gaacgttatt ttgcgccgcg cggcattgcg
     3241 atgctgacta ttgatatgcc gtcggtgggc ttttcttcaa aatggaagct cacccaggac
     3301 tccagcctgt tgcatcagca cgtcttaaag gcgctgccta acgtaccgtg ggtggatcac
     3361 actcgcgtcg cggcctttgg tttccgtttc ggcgctaacg ttgccgtgcg tctggcatac
     3421 cttgaatcgc cgcg
//
LOCUS       DAJTQY010000315         3198 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.guided.209, whole genome shotgun sequence.
ACCESSION   DAJTQY010000315 DAJTQY010000000
VERSION     DAJTQY010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3198
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.guided.209"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            77..481
                     /locus_tag="OO349_004217"
     CDS             77..481
                     /locus_tag="OO349_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000512977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HCR5869058.1"
                     /translation="MGISLNHGVHAKVSTPVHLRKARTCYDHLAGEVAVKIYDSLCQQ
                     QWITENGSMITLSGIQYFHEMGIDVPSKHSRKICCACLDWSERRFHLGGYVGAALFSL
                     YESKGWLTRHLGYREVTITEKGYAAFKTHFHI"
     gene            complement(459..1082)
                     /gene="tetR(B)"
                     /locus_tag="OO349_004218"
     CDS             complement(459..1082)
                     /gene="tetR(B)"
                     /locus_tag="OO349_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HCR5869059.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            1164..2369
                     /gene="tet(B)"
                     /locus_tag="OO349_004219"
     CDS             1164..2369
                     /gene="tet(B)"
                     /locus_tag="OO349_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HCR5869060.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(2482..3075)
                     /gene="tetC"
                     /locus_tag="OO349_004220"
     CDS             complement(2482..3075)
                     /gene="tetC"
                     /locus_tag="OO349_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HCR5869061.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
BASE COUNT          855 a          595 c          659 g         1089 t
ORIGIN      
        1 cacagtagta gctcaaggca agcatcgtta ttttcggcta gcaggaaaag atattgctga
       61 attgatggaa agtatgatgg ggatctcctt aaaccatggc gtacatgcca aagtttccac
      121 gccagtgcat ttacgaaaag cacgtacttg ctatgatcat ttagctggcg aagttgccgt
      181 taagatctat gattcccttt gtcaacagca atggatcact gaaaatggtt caatgatcac
      241 attaagtggt attcaatatt ttcatgaaat gggaattgac gttccttcca aacattcacg
      301 taaaatctgt tgtgcgtgtt tagattggag tgaacgccgt ttccatttag gtgggtacgt
      361 tggagccgca ttattttcgc tttatgaatc taaagggtgg ttaactcgac atcttggtta
      421 ccgtgaagtt accatcacgg aaaaaggtta tgctgctttt aagacccact ttcacattta
      481 agttgttttt ctaatccgca tatgatcaat tcaaggccga ataagaaggc tggctctgca
      541 ccttggtgat caaataattc gatagcttgt cgtaataatg gcggcatact atcagtagta
      601 ggtgtttccc tttcttcttt agcgacttga tgctcttgat cttccaatac gcaacctaaa
      661 gtaaaatgcc ccacagcgct gagtgcatat aatgcattct ctagtgaaaa accttgttgg
      721 cataaaaagg ctaattgatt ttcgagagtt tcatactgtt tttctgtagg ccgtgtacct
      781 aaatgtactt ttgctccatc gcgatgactt agtaaagcac atctaaaact tttagcgtta
      841 ttacgtaaaa aatcttgcca gctttcccct tctaaagggc aaaagtgagt atggtgccta
      901 tctaacatct caatggctaa ggcgtcgagc aaagcccgct tattttttac atgccaatac
      961 aatgtaggct gctctacacc tagcttctgg gcgagtttac gggttgttaa accttcgatt
     1021 ccgacctcat taagcagctc taatgcgctg ttaatcactt tacttttatc taatctagac
     1081 atcattaatt cctaattttt gttgacactc tatcattgat agagttattt taccactccc
     1141 tatcagtgat agagaaaagt gaaatgaata gttcgacaaa gatcgcattg gtaattacgt
     1201 tactcgatgc catggggatt ggccttatca tgccagtctt gccaacgtta ttacgtgaat
     1261 ttattgcttc ggaagatatc gctaaccact ttggcgtatt gcttgcactt tatgcgttaa
     1321 tgcaggttat ctttgctcct tggcttggaa aaatgtctga ccgatttggt cggcgcccag
     1381 tgctgttgtt gtcattaata ggcgcatcgc tggattactt attgctggct ttttcaagtg
     1441 cgctttggat gctgtattta ggccgtttgc tttcagggat cacaggagct actggggctg
     1501 tcgcggcatc ggtcattgcc gataccacct cagcttctca acgcgtgaag tggttcggtt
     1561 ggttaggggc aagttttggg cttggtttaa tagcggggcc tattattggt ggttttgcag
     1621 gagagatttc accgcatagt ccctttttta tcgctgcgtt gctaaatatt gtcgctttcc
     1681 ttgtggttat gttttggttc cgtgaaacca aaaatacacg tgataataca gataccgaag
     1741 taggggttga gacgcaatcg aattcggtat acatcacttt atttaaaacg atgcccattt
     1801 tgttgattat ttatttttca gcgcaattga taggccaaat tcccgcaacg gtgtgggtgc
     1861 tatttaccga aaatcgtttt ggatggaata gcatgatggt tggcttttca ttagcgggtc
     1921 ttggtctttt acactcagta ttccaagcct ttgtggcagg aagaatagcc actaaatggg
     1981 gcgaaaaaac ggcagtactg ctcggattta ttgcagatag tagtgcattt gcctttttag
     2041 cgtttatatc tgaaggttgg ttagttttcc ctgttttaat tttattggct ggtggtggga
     2101 tcgctttacc tgcattacag ggagtgatgt ctatccaaac aaagagtcat cagcaaggtg
     2161 ctttacaggg attattggtg agccttacca atgcaaccgg tgttattggc ccattactgt
     2221 ttgctgttat ttataatcat tcactaccaa tttgggatgg ctggatttgg attattggtt
     2281 tagcgtttta ctgtattatt atcctgctat cgatgacctt catgttaacc cctcaagctc
     2341 aggggagtaa acaggagaca agtgcttagt tatttcgtca ccaaatgatg ttattccgcg
     2401 aaatataatg accctcttga taacccaaga gggcattttt tacgataaag aagatttagc
     2461 ttcaaataaa acctatctat tttatttatc tttcaagctc aataaaaagc cgcggtaaat
     2521 agcaataaat tggccttttt tatcggcaag ctcttttagg tttttcgcat gtattgcgat
     2581 atgcataaac cagccattga gtaagttttt aagcacatca tcatcataag ctttaagttg
     2641 gttctcttgg atcaatttgc tgacaatggc gtttacctta ccagtaatgt attcaaggct
     2701 aattttttca agttcattcc aaccaatgat aggcatcact tcttggatag ggataaggtt
     2761 tttattatta tcaataatat aatcaagata atgttcaaat atactttcta aggcagacca
     2821 accatttgtt aaatcagttt ttgttgtgat gtaggcatca atcataatta attgctgctt
     2881 ataacaggca ctgagtaatt gttttttatt tttaaagtga tgataaaagg cacctttggt
     2941 caccaacgct tttcccgaga tctcatctat tgaaacagct tgatagcctt tttcaacaaa
     3001 caatattcgt gctgagttaa ccagtgattg ataggtactc ttaaaatttt cttgttgatg
     3061 atttttattt tccatgatag atttaaaata acataccgtc agtatgttta tggtatcatg
     3121 atgatgtggt cgtgacaatc ttaagaacat ttaggttatt ttatgtatat tgaacagcat
     3181 tctcgctatc aaaataaa
//
LOCUS       DAJTQY010000316         2493 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.guided.220, whole genome shotgun sequence.
ACCESSION   DAJTQY010000316 DAJTQY010000000
VERSION     DAJTQY010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2493
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.guided.220"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(<1..160)
                     /locus_tag="OO349_004221"
     CDS             complement(<1..160)
                     /locus_tag="OO349_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023994230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase N-terminal SAM-like
                     domain-containing protein"
                     /protein_id="HCR5869062.1"
                     /translation="MSNSPFLNSIRTDMRQKGYALKTEKTYLHWIKRFILFHKKRHPQ
                     TMGSEEVRL"
     gene            492..965
                     /locus_tag="OO349_004222"
     CDS             492..965
                     /locus_tag="OO349_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HCR5869063.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            1060..1584
                     /gene="sat2"
                     /locus_tag="OO349_004223"
     CDS             1060..1584
                     /gene="sat2"
                     /locus_tag="OO349_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HCR5869064.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            1642..2433
                     /gene="aadA1"
                     /locus_tag="OO349_004224"
     CDS             1642..2433
                     /gene="aadA1"
                     /locus_tag="OO349_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR5869065.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT          695 a          555 c          603 g          640 t
ORIGIN      
        1 acagcctgac ctcttcactg cccatggtct gaggatgacg ttttttgtga aacagaataa
       61 aacgcttaat ccagtgcagg taagtttttt cagttttcag cgcataacct ttttgtcgca
      121 tatccgtgcg tatagaattt aaaaatggac tgttagacat aaaacgctcc ttgtcttgca
      181 actgtctgcc tatacagcct attctagctg ggatttaaaa aagtgcctgt tttttacgcc
      241 tagagatgct tgtttaccgg tagagtttta atttaatgct aaataaatta aaatgttatg
      301 agttctttgg gtgagataat gtgcatcgtg caagcaggat agacggcatg cacgatttgt
      361 aataacagag tgtcttgtat ttttaaagaa agtctattta atacaagtga ttatattaat
      421 taacggtaag catcagcggg tgacaaaacg agcatgctta ctaataaaat gttaacctct
      481 gaggaagaat tgtgaaacta tcactaatgg tagctatatc gaagaatgga gttatcggga
      541 atggccctga tattccatgg agtgccaaag gtgaacagct cctgtttaaa gctattacct
      601 ataaccaatg gctgttggtt ggacgcaaga cttttgaatc aatgggagca ttacccaacc
      661 gaaagtatgc ggtcgtaaca cgttcaagtt ttacatctga caatgagaac gtattgatct
      721 ttccatcaat taaagatgct ttaaccaacc taaagaaaat aacggatcat gtcattgttt
      781 caggtggtgg ggagatatac aaaagcctga tcgatcaagt agatacacta catatatcta
      841 caatagacat cgagccggaa ggtgatgttt actttcctga aatccccagc aattttaggc
      901 cagtttttac ccaagacttc gcctctaaca taaattatag ttaccaaatc tggcaaaagg
      961 gttaacaagt ggcagcaacg gattcgcaaa cctgtcacgc cttttgtacc aaaagccgcg
     1021 ccaggtttgc gatccgctgt gccaggcgtt aggcgtcata tgaagatttc ggtgatccct
     1081 gagcaggtgg cggaaacatt ggatgctgag aaccatttca ttgttcgtga agtgttcgat
     1141 gtgcacctat ccgaccaagg ctttgaacta tctaccagaa gtgtgagccc ctaccggaag
     1201 gattacatct cggatgatga ctctgatgaa gactctgctt gctatggcgc attcatcgac
     1261 caagagcttg tcgggaagat tgaactcaac tcaacatgga acgatctagc ctctatcgaa
     1321 cacattgttg tgtcgcacac gcaccgaggc aaaggagtcg cgcacagtct catcgaattt
     1381 gcgaaaaagt gggcactaag cagacagctc cttggcatac gattagagac acaaacgaac
     1441 aatgtacctg cctgcaattt gtacgcaaaa tgtggcttta ctctcggcgg cattgacctg
     1501 ttcacgtata aaactagacc tcaagtctcg aacgaaacag cgatgtactg gtactggttc
     1561 tcgggagcac aggatgacgc ctaacaattc attcaagccg acaccgcttc gcggcgcggc
     1621 ttaattcagg agttaaacat catgagggaa gcggtgatcg ccgaagtatc gactcaacta
     1681 tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg
     1741 tacggctccg cagtggatgg cggcctgaag ccacacagtg atattgattt gctggttacg
     1801 gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact
     1861 tcggcttccc ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac
     1921 gacgacatca ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag
     1981 cgcaatgaca ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc
     2041 ttgctgacaa aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc
     2101 tttgatccgg ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg
     2161 aactcgccgc ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt
     2221 tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg
     2281 gagcgcctgc cggcccagta tcagcccgtc atacttgaag ctagacaggc ttatcttgga
     2341 caagaagaag atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt tcactacgtg
     2401 aaaggcgaga tcaccaaggt agtcggcaaa taatgtctaa caattcgttc aagccgacgc
     2461 cgcttcgcgg cgcggcttaa ctcaagcgtt aga
//
LOCUS       DAJTQY010000317         2490 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.guided.221, whole genome shotgun sequence.
ACCESSION   DAJTQY010000317 DAJTQY010000000
VERSION     DAJTQY010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2490
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.guided.221"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(<1..160)
                     /locus_tag="OO349_004225"
     CDS             complement(<1..160)
                     /locus_tag="OO349_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023994230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase N-terminal SAM-like
                     domain-containing protein"
                     /protein_id="HCR5869066.1"
                     /translation="MSNSPFLNSIRTDMRQKGYALKTEKTYLHWIKRFILFHKKRHPQ
                     TMGSEEVRL"
     gene            492..965
                     /locus_tag="OO349_004226"
     CDS             492..965
                     /locus_tag="OO349_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HCR5869067.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            1060..1584
                     /gene="sat2"
                     /locus_tag="OO349_004227"
     CDS             1060..1584
                     /gene="sat2"
                     /locus_tag="OO349_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HCR5869068.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            1642..2430
                     /gene="aadA1"
                     /locus_tag="OO349_004228"
     CDS             1642..2430
                     /gene="aadA1"
                     /locus_tag="OO349_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR5869069.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT          692 a          555 c          603 g          640 t
ORIGIN      
        1 acagcctgac ctcttcactg cccatggtct gaggatgacg ttttttgtga aacagaataa
       61 aacgcttaat ccagtgcagg taagtttttt cagttttcag cgcataacct ttttgtcgca
      121 tatccgtgcg tatagaattt aaaaatggac tgttagacat aaaacgctcc ttgtcttgca
      181 actgtctgcc tatacagcct attctagctg ggatttaaaa aagtgcctgt tttttacgcc
      241 tagagatgct tgtttaccgg tagagtttta atttaatgct aaataaatta aaatgttatg
      301 agttctttgg gtgagataat gtgcatcgtg caagcaggat agacggcatg cacgatttgt
      361 aataacagag tgtcttgtat ttttaaagaa agtctattta atacaagtga ttatattaat
      421 taacggtaag catcagcggg tgacaaaacg agcatgctta ctaataaaat gttaacctct
      481 gaggaagaat tgtgaaacta tcactaatgg tagctatatc gaagaatgga gttatcggga
      541 atggccctga tattccatgg agtgccaaag gtgaacagct cctgtttaaa gctattacct
      601 ataaccaatg gctgttggtt ggacgcaaga cttttgaatc aatgggagca ttacccaacc
      661 gaaagtatgc ggtcgtaaca cgttcaagtt ttacatctga caatgagaac gtattgatct
      721 ttccatcaat taaagatgct ttaaccaacc taaagaaaat aacggatcat gtcattgttt
      781 caggtggtgg ggagatatac aaaagcctga tcgatcaagt agatacacta catatatcta
      841 caatagacat cgagccggaa ggtgatgttt actttcctga aatccccagc aattttaggc
      901 cagtttttac ccaagacttc gcctctaaca taaattatag ttaccaaatc tggcaaaagg
      961 gttaacaagt ggcagcaacg gattcgcaaa cctgtcacgc cttttgtacc aaaagccgcg
     1021 ccaggtttgc gatccgctgt gccaggcgtt aggcgtcata tgaagatttc ggtgatccct
     1081 gagcaggtgg cggaaacatt ggatgctgag aaccatttca ttgttcgtga agtgttcgat
     1141 gtgcacctat ccgaccaagg ctttgaacta tctaccagaa gtgtgagccc ctaccggaag
     1201 gattacatct cggatgatga ctctgatgaa gactctgctt gctatggcgc attcatcgac
     1261 caagagcttg tcgggaagat tgaactcaac tcaacatgga acgatctagc ctctatcgaa
     1321 cacattgttg tgtcgcacac gcaccgaggc aaaggagtcg cgcacagtct catcgaattt
     1381 gcgaaaaagt gggcactaag cagacagctc cttggcatac gattagagac acaaacgaac
     1441 aatgtacctg cctgcaattt gtacgcaaaa tgtggcttta ctctcggcgg cattgacctg
     1501 ttcacgtata aaactagacc tcaagtctcg aacgaaacag cgatgtactg gtactggttc
     1561 tcgggagcac aggatgacgc ctaacaattc attcaagccg acaccgcttc gcggcgcggc
     1621 ttaattcagg agttaaacat catgagggaa gcggtgatcg ccgaagtatc gactcaacta
     1681 tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg
     1741 tacggctccg cagtggatgg cggcctgaag ccacacagtg atattgattt gctggttacg
     1801 gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact
     1861 tcggcttccc ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac
     1921 gacgacatca ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag
     1981 cgcaatgaca ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc
     2041 ttgctgacaa aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc
     2101 tttgatccgg ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg
     2161 aactcgccgc ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt
     2221 tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg
     2281 gagcgcctgc cggcccagta tcagcccgtc atacttgaag ctaggcaggc ttatcttgga
     2341 caagaagatc gcttggcctc gcgcgcagat cagttggaag aatttgttca ctacgtgaaa
     2401 ggcgagatca ccaaggtagt cggcaaataa tgtctaacaa ttcgttcaag ccgacgccgc
     2461 ttcgcggcgc ggcttaactc aagcgttaga
//
LOCUS       DAJTQY010000318         1653 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.guided.239, whole genome shotgun sequence.
ACCESSION   DAJTQY010000318 DAJTQY010000000
VERSION     DAJTQY010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.guided.239"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            complement(<1..450)
                     /gene="intI1"
                     /locus_tag="OO349_004229"
     CDS             complement(<1..450)
                     /gene="intI1"
                     /locus_tag="OO349_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HCR5869070.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEG"
     gene            660..1490
                     /locus_tag="OO349_004230"
     CDS             660..1490
                     /locus_tag="OO349_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-1"
                     /protein_id="HCR5869071.1"
                     /translation="MKNTIHINFAIFLIIANIIYSSASASTDISTVASPLFEGTEGCF
                     LLYDASTNAEIAQFNKAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGM
                     EIWNSNHTPKTWMQFSVVWVSQEITQKIGLNKIKNYLKDFDYGNQDFSGDKERNNGLT
                     EAWLESSLKISPEEQIQFLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGA
                     GFTANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL
                     "
BASE COUNT          532 a          379 c          356 g          386 t
ORIGIN      
        1 accctcactg atccgcatgc ccgttccata cagaagctgg gcgaacaaac gatgctcgcc
       61 ttccagaaaa ccgaggatgc gaaccacttc atccggggtc agcaccaccg gcaagcgccg
      121 cgacggccga ggtcttccga tctcctgaag ccagggcaga tccgtgcaca gcaccttgcc
      181 gtagaagaac agcaaggccg ccaatgcctg acgatgcgtg gagaccgaaa ccttgcgctc
      241 gttcgccagc caggacagaa atgcctcgac ttcgctgctg cccaaggttg ccgggtgacg
      301 cacaccgtgg aaacggatga aggcacgaac ccagtggaca taagcctgtt cggttcgtaa
      361 gctgtaatgc aagtagcgta tgcgctcacg caactggtcc agaaccttga ccgaacgcag
      421 cggtggtaac ggcgcagtgg cggttttcat ggcttgttat gactgttttt ttggggtaca
      481 gtctatgcct cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt
      541 atggagcagc aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt tgggcgaacc
      601 cggagcctca ttaattgtta gccgttaaaa ttaagccctt taccaaacca atacttatta
      661 tgaaaaacac aatacatatc aacttcgcta tttttttaat aattgcaaat attatctaca
      721 gcagcgccag tgcatcaaca gatatctcta ctgttgcatc tccattattt gaaggaactg
      781 aaggttgttt tttactttac gatgcatcca caaacgctga aattgctcaa ttcaataaag
      841 caaagtgtgc aacgcaaatg gcaccagatt caactttcaa gatcgcatta tcacttatgg
      901 catttgatgc ggaaataata gatcagaaaa ccatattcaa atgggataaa acccccaaag
      961 gaatggagat ctggaacagc aatcatacac caaagacgtg gatgcaattt tctgttgttt
     1021 gggtttcgca agaaataacc caaaaaattg gattaaataa aatcaagaat tatctcaaag
     1081 attttgatta tggaaatcaa gacttctctg gagataaaga aagaaacaac ggattaacag
     1141 aagcatggct cgaaagtagc ttaaaaattt caccagaaga acaaattcaa ttcctgcgta
     1201 aaattattaa tcacaatctc ccagttaaaa actcagccat agaaaacacc atagagaaca
     1261 tgtatctaca agatctggat aatagtacaa aactgtatgg gaaaactggt gcaggattca
     1321 cagcaaatag aaccttacaa aacggatggt ttgaagggtt tattataagc aaatcaggac
     1381 ataaatatgt ttttgtgtcc gcacttacag gaaacttggg gtcgaattta acatcaagca
     1441 taaaagccaa gaaaaatgcg atcaccattc taaacacact aaatttataa aaaatctaat
     1501 ggcaaaatcg cccaaccctt caatcaagtc gggacggcca aaagcaagct tttggctccc
     1561 ctcgctggcg ctcggcgccc cttatttcaa acgttaaaca tcatgaggga agtggtgatc
     1621 gccgaagtat cgactcaact atcagaggta gtt
//
LOCUS       DAJTQY010000319         1335 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.guided.249, whole genome shotgun sequence.
ACCESSION   DAJTQY010000319 DAJTQY010000000
VERSION     DAJTQY010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1335
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.guided.249"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            264..923
                     /locus_tag="OO349_004231"
     CDS             264..923
                     /locus_tag="OO349_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HCR5869072.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
     gene            complement(1124..>1335)
                     /locus_tag="OO349_004232"
     CDS             complement(1124..>1335)
                     /locus_tag="OO349_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5869073.1"
                     /translation="DRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQAL
                     GGRINDHIVNYYLHGESLSKLASGI"
BASE COUNT          352 a          297 c          314 g          372 t
ORIGIN      
        1 acaccatcat acactaaatc agtaagttgg cagcatcacc cgacgcactt tgcgccgaat
       61 aaatacctgt gacggaagat cacttcgcag aataaataaa tcctggtgtc cctgttgata
      121 ccgggaagcc ctgggccaac ttttggcgaa aatgagacgt tgatcggcac gtaagaggtt
      181 ccaactttca ccataatgaa ataagatcac taccgggcgt attttttgag ttatcgagat
      241 tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc accgttgata
      301 tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct caatgtacct
      361 ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag aaaaataagc
      421 acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct catccggaat
      481 tccgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac ccttgttaca
      541 ccgttttcca tgagcaaact gaaacgtttt catcgctctg gagtgaatac cacgacgatt
      601 tccggcagtt tctacacata tattcgcaag atgtggcgtg ttacggtgaa aacctggcct
      661 atttccctaa agggtttatt gagaatatgt ttttcgtctc agccaatccc tgggtgagtt
      721 tcaccagttt tgatttaaac gtggccaata tggacaactt cttcgccccc gttttcacca
      781 tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt caggttcatc
      841 atgccgtttg tgatggcttc catgtcggca gaatgcttaa tgaattacaa cagtactgcg
      901 atgagtggca gggcggggcg taattttttt aaggcagtta ttggtgccct taaacgcctg
      961 gttgctacgc ctgaataagt gataataagc ggatgaatgg cagaaattcg aaagcaaatt
     1021 cgacccggtc gtcggttcag ggcagggtcg ttaaatagcc gcttatgtct attgctggtt
     1081 taccggttta ttgactaccg gaagcagtgt gaccgtgtgc ttctcaaatg cctgaggcca
     1141 gtttgctcag gctctccccg tggaggtaat aattgacgat atgatcattt attctgcctc
     1201 ccagagcctg ataaaaacgg ttagcgccgg ggttggattt ttcagcgttc cagctaaggc
     1261 taaggcattc ctgttcaagc gcaagccggg ctataaagcg cattatcgct ttacccgtgc
     1321 ccttatttcg atcgc
//
LOCUS       DAJTQY010000320         1101 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.guided.259, whole genome shotgun sequence.
ACCESSION   DAJTQY010000320 DAJTQY010000000
VERSION     DAJTQY010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1101
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.guided.259"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            250..1041
                     /gene="aadA1"
                     /locus_tag="OO349_004233"
     CDS             250..1041
                     /gene="aadA1"
                     /locus_tag="OO349_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR5869074.1"
                     /translation="MREVVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT          294 a          270 c          283 g          254 t
ORIGIN      
        1 aagggtttat tataagcaaa tcaggacata aatatgtttt tgtgtccgca cttacaggaa
       61 acttggggtc gaatttaaca tcaagcataa aagccaagaa aaatgcgatc accattctaa
      121 acacactaaa tttataaaaa atctaatggc aaaatcgccc aacccttcaa tcaagtcggg
      181 acggccaaaa gcaagctttt ggctcccctc gctggcgctc ggcgcccctt atttcaaacg
      241 ttaaacatca tgagggaagt ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt
      301 ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg tacatttgta cggctccgca
      361 gtggatggcg gcctgaagcc acacagtgat attgatttgc tggttacggt gaccgtaagg
      421 cttgatgaaa caacgcggcg agctttgatc aacgaccttt tggaaacttc ggcttcccct
      481 ggagagagcg agattctccg cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt
      541 ccgtggcgtt atccagctaa gcgcgaactg caatttggag aatggcagcg caatgacatt
      601 cttgcaggta tcttcgagcc agccacgatc gacattgatc tggctatctt gctgacaaaa
      661 gcaagagaac atagcgttgc cttggtaggt ccagcggcgg aggaactctt tgatccggtt
      721 cctgaacagg atctatttga ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc
      781 gactgggctg gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg gtacagcgca
      841 gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga gcgcctgccg
      901 gcccagtatc agcccgtcat acttgaagct agacaggctt atcttggaca agaagaagat
      961 cgcttggcct cgcgcgcaga tcagttggaa gaatttgttc actacgtgaa aggcgagatc
     1021 accaaggtag tcggcaaata atgtctaaca attcgttcaa gccgacgccg cttcgcggcg
     1081 cggcttaact caagcgttag a
//
LOCUS       DAJTQY010000321         1098 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 401781
            SAMEA8900037-rid15818833.guided.260, whole genome shotgun sequence.
ACCESSION   DAJTQY010000321 DAJTQY010000000
VERSION     DAJTQY010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900037
            Sequence Read Archive: ERR6004918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477832.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:07:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,234
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 3,835
            CDSs (with protein)               :: 3,835
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 148 of 321
            Pseudo Genes (incomplete)         :: 61 of 321
            Pseudo Genes (internal stop)      :: 139 of 321
            Pseudo Genes (multiple problems)  :: 26 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1098
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900037-rid15818833.guided.260"
                     /serovar="S. flexneri 1b"
                     /isolate="401781"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Kenya"
                     /collection_date="2008-11-21"
     gene            250..1038
                     /gene="aadA1"
                     /locus_tag="OO349_004234"
     CDS             250..1038
                     /gene="aadA1"
                     /locus_tag="OO349_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR5869075.1"
                     /translation="MREVVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT          291 a          270 c          283 g          254 t
ORIGIN      
        1 aagggtttat tataagcaaa tcaggacata aatatgtttt tgtgtccgca cttacaggaa
       61 acttggggtc gaatttaaca tcaagcataa aagccaagaa aaatgcgatc accattctaa
      121 acacactaaa tttataaaaa atctaatggc aaaatcgccc aacccttcaa tcaagtcggg
      181 acggccaaaa gcaagctttt ggctcccctc gctggcgctc ggcgcccctt atttcaaacg
      241 ttaaacatca tgagggaagt ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt
      301 ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg tacatttgta cggctccgca
      361 gtggatggcg gcctgaagcc acacagtgat attgatttgc tggttacggt gaccgtaagg
      421 cttgatgaaa caacgcggcg agctttgatc aacgaccttt tggaaacttc ggcttcccct
      481 ggagagagcg agattctccg cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt
      541 ccgtggcgtt atccagctaa gcgcgaactg caatttggag aatggcagcg caatgacatt
      601 cttgcaggta tcttcgagcc agccacgatc gacattgatc tggctatctt gctgacaaaa
      661 gcaagagaac atagcgttgc cttggtaggt ccagcggcgg aggaactctt tgatccggtt
      721 cctgaacagg atctatttga ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc
      781 gactgggctg gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg gtacagcgca
      841 gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga gcgcctgccg
      901 gcccagtatc agcccgtcat acttgaagct aggcaggctt atcttggaca agaagatcgc
      961 ttggcctcgc gcgcagatca gttggaagaa tttgttcact acgtgaaagg cgagatcacc
     1021 aaggtagtcg gcaaataatg tctaacaatt cgttcaagcc gacgccgctt cgcggcgcgg
     1081 cttaactcaa gcgttaga
//
